In and Out 101 Activities to Enrich the Learning Experience.

ERIC Educational Resources Information Center

Johnson, Patricia; And Others

Activities developed and used with children and adults participating in the program offerings of the Edwin Gould Outdoor Education Centers are presented. Information describing most activities includes name, description of the activity, objectives, supervision or help required, procedures, time involved, size of area required, materials,…

Developing design-based STEM education learning activities to enhance students' creative thinking

NASA Astrophysics Data System (ADS)

Pinasa, Siwa; Siripun, Kulpatsorn; Yuenyong, Chokchai

2018-01-01

Creative thinking on applying science and mathematics knowledge is required by the future STEM career. The STEM education should be provided for the required skills of future STEM career. This paper aimed to clarify the developing STEM education learning activities to enhance students' creative thinking. The learning activities were developed for Grade 10 students who will study in the subject of independent study (IS) of Khon Kaen Wittayayon School, Khon Kaen, Thailand. The developing STEM education learning activities for enhancing students' creative thinking was developed regarding on 6 steps including (1) providing of understanding of fundamental STEM education concept, (2) generating creative thinking from prototype, (4) revised ideas, (5) engineering ability, and (6) presentation and discussion. The paper will clarify the 18 weeks activities that will be provided based these 6 steps of developing learning activities. Then, these STEM learning activities will be discussed to provide the chance of enhancing students' creative thinking. The paper may have implication for STEM education in school setting.

24 CFR 1000.542 - When may HUD require replacement of a recipient?

Code of Federal Regulations, 2013 CFR

2013-04-01

..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring... practice of activities that constitute substantial or willful noncompliance with the requirements of...

24 CFR 1000.542 - When may HUD require replacement of a recipient?

Code of Federal Regulations, 2012 CFR

2012-04-01

..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring... practice of activities that constitute substantial or willful noncompliance with the requirements of...

24 CFR 1000.542 - When may HUD require replacement of a recipient?

Code of Federal Regulations, 2014 CFR

2014-04-01

..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring... practice of activities that constitute substantial or willful noncompliance with the requirements of...

24 CFR 1000.542 - When may HUD require replacement of a recipient?

Code of Federal Regulations, 2010 CFR

2010-04-01

..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring... practice of activities that constitute substantial or willful noncompliance with the requirements of...

24 CFR 1000.542 - When may HUD require replacement of a recipient?

Code of Federal Regulations, 2011 CFR

2011-04-01

..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring... practice of activities that constitute substantial or willful noncompliance with the requirements of...

Using model based systems engineering for the development of the Large Synoptic Survey Telescope's operational plan

NASA Astrophysics Data System (ADS)

Selvy, Brian M.; Claver, Charles; Willman, Beth; Petravick, Don; Johnson, Margaret; Reil, Kevin; Marshall, Stuart; Thomas, Sandrine; Lotz, Paul; Schumacher, German; Lim, Kian-Tat; Jenness, Tim; Jacoby, Suzanne; Emmons, Ben; Axelrod, Tim

2016-08-01

We† provide an overview of the Model Based Systems Engineering (MBSE) language, tool, and methodology being used in our development of the Operational Plan for Large Synoptic Survey Telescope (LSST) operations. LSST's Systems Engineering (SE) team is using a model-based approach to operational plan development to: 1) capture the topdown stakeholders' needs and functional allocations defining the scope, required tasks, and personnel needed for operations, and 2) capture the bottom-up operations and maintenance activities required to conduct the LSST survey across its distributed operations sites for the full ten year survey duration. To accomplish these complimentary goals and ensure that they result in self-consistent results, we have developed a holistic approach using the Sparx Enterprise Architect modeling tool and Systems Modeling Language (SysML). This approach utilizes SysML Use Cases, Actors, associated relationships, and Activity Diagrams to document and refine all of the major operations and maintenance activities that will be required to successfully operate the observatory and meet stakeholder expectations. We have developed several customized extensions of the SysML language including the creation of a custom stereotyped Use Case element with unique tagged values, as well as unique association connectors and Actor stereotypes. We demonstrate this customized MBSE methodology enables us to define: 1) the rolls each human Actor must take on to successfully carry out the activities associated with the Use Cases; 2) the skills each Actor must possess; 3) the functional allocation of all required stakeholder activities and Use Cases to organizational entities tasked with carrying them out; and 4) the organization structure required to successfully execute the operational survey. Our approach allows for continual refinement utilizing the systems engineering spiral method to expose finer levels of detail as necessary. For example, the bottom-up, Use Case-driven approach will be deployed in the future to develop the detailed work procedures required to successfully execute each operational activity.

Validation Methods Research for Fault-Tolerant Avionics and Control Systems: Working Group Meeting, 2

NASA Technical Reports Server (NTRS)

Gault, J. W. (Editor); Trivedi, K. S. (Editor); Clary, J. B. (Editor)

1980-01-01

The validation process comprises the activities required to insure the agreement of system realization with system specification. A preliminary validation methodology for fault tolerant systems documented. A general framework for a validation methodology is presented along with a set of specific tasks intended for the validation of two specimen system, SIFT and FTMP. Two major areas of research are identified. First, are those activities required to support the ongoing development of the validation process itself, and second, are those activities required to support the design, development, and understanding of fault tolerant systems.

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development

PubMed Central

Ciarlo, Christie; Kaufman, Charles K; Kinikoglu, Beste; Michael, Jonathan; Yang, Song; D′Amato, Christopher; Blokzijl-Franke, Sasja; den Hertog, Jeroen; Schlaeger, Thorsten M; Zhou, Yi; Liao, Eric

2017-01-01

The neural crest is a dynamic progenitor cell population that arises at the border of neural and non-neural ectoderm. The inductive roles of FGF, Wnt, and BMP at the neural plate border are well established, but the signals required for subsequent neural crest development remain poorly characterized. Here, we conducted a screen in primary zebrafish embryo cultures for chemicals that disrupt neural crest development, as read out by crestin:EGFP expression. We found that the natural product caffeic acid phenethyl ester (CAPE) disrupts neural crest gene expression, migration, and melanocytic differentiation by reducing Sox10 activity. CAPE inhibits FGF-stimulated PI3K/Akt signaling, and neural crest defects in CAPE-treated embryos are suppressed by constitutively active Akt1. Inhibition of Akt activity by constitutively active PTEN similarly decreases crestin expression and Sox10 activity. Our study has identified Akt as a novel intracellular pathway required for neural crest differentiation. PMID:28832322

Space and Missile Systems Center Standard: Software Development

DTIC Science & Technology

2015-01-16

maintenance , or any other activity or combination of activities resulting in products . Within this standard, requirements to “develop,” “define...integration, reuse, reengineering, maintenance , or any other activity that results in products ). The term “developer” encompasses all software team...activities that results in software products . Software development includes new development, modification, reuse, reengineering, maintenance , and any other

24 CFR 92.358 - Consultant activities.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Consultant activities. 92.358 Section 92.358 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development HOME INVESTMENT PARTNERSHIPS PROGRAM Other Federal Requirements § 92.358 Consultant activities. No...

24 CFR 92.358 - Consultant activities.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Consultant activities. 92.358 Section 92.358 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development HOME INVESTMENT PARTNERSHIPS PROGRAM Other Federal Requirements § 92.358 Consultant activities. No...

24 CFR 92.358 - Consultant activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Consultant activities. 92.358 Section 92.358 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development HOME INVESTMENT PARTNERSHIPS PROGRAM Other Federal Requirements § 92.358 Consultant activities. No...

24 CFR 92.358 - Consultant activities.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Consultant activities. 92.358 Section 92.358 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development HOME INVESTMENT PARTNERSHIPS PROGRAM Other Federal Requirements § 92.358 Consultant activities. No...

24 CFR 92.358 - Consultant activities.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Consultant activities. 92.358 Section 92.358 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development HOME INVESTMENT PARTNERSHIPS PROGRAM Other Federal Requirements § 92.358 Consultant activities. No...

Proximity operations concept design study, task 6

NASA Technical Reports Server (NTRS)

Williams, A. N.

1990-01-01

The feasibility of using optical technology to perform the mission of the proximity operations communications subsystem on Space Station Freedom was determined. Proximity operations mission requirements are determined and the relationship to the overall operational environment of the space station is defined. From this information, the design requirements of the communication subsystem are derived. Based on these requirements, a preliminary design is developed and the feasibility of implementation determined. To support the Orbital Maneuvering Vehicle and National Space Transportation System, the optical system development is straightforward. The requirements on extra-vehicular activity are such as to allow large fields of uncertainty, thus exacerbating the acquisition problem; however, an approach is given that could mitigate this problem. In general, it is found that such a system could indeed perform the proximity operations mission requirement, with some development required to support extra-vehicular activity.

Development of flight experiment task requirements. Volume 1: Summary

NASA Technical Reports Server (NTRS)

Hatterick, G. R.

1972-01-01

A study was conducted to develop the means to identify skills required of scientist passengers on advanced missions related to the space shuttle and RAM programs. The scope of the study was defined to include only the activities of on-orbit personnel which are directly related to, or required by, on-orbit experimentation and scientific investigations conducted on or supported by the shuttle orbiter. A program summary is presented which provides a description of the methodology developed, an overview of the activities performed during the study, and the results obtained through application of the methodology.

24 CFR 1000.42 - Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable? 1000.42 Section 1000.42 Housing and... ACTIVITIES General § 1000.42 Are the requirements of section 3 of the Housing and Urban Development Act of...

32 CFR 37.625 - What cost principles or standards do I require for for-profit participants?

Code of Federal Regulations, 2012 CFR

2012-07-01

... research and development activities under the Generally Accepted Accounting Principles (see Statement of Financial Accounting Standards Number 2, “Accounting for Research and Development Costs,” October 1974 1... the participants' other research and development activities (e.g., activities supported by the...

Advanced extravehicular activity systems requirements definition study. Phase 2: Extravehicular activity at a lunar base

NASA Technical Reports Server (NTRS)

Neal, Valerie; Shields, Nicholas, Jr.; Carr, Gerald P.; Pogue, William; Schmitt, Harrison H.; Schulze, Arthur E.

1988-01-01

The focus is on Extravehicular Activity (EVA) systems requirements definition for an advanced space mission: remote-from-main base EVA on the Moon. The lunar environment, biomedical considerations, appropriate hardware design criteria, hardware and interface requirements, and key technical issues for advanced lunar EVA were examined. Six remote EVA scenarios (three nominal operations and three contingency situations) were developed in considerable detail.

An Integrated Approach to Exploration Launch Office Requirements Development

NASA Technical Reports Server (NTRS)

Holladay, Jon B.; Langford, Gary

2006-01-01

The proposed paper will focus on the Project Management and Systems Engineering approach utilized to develop a set of both integrated and cohesive requirements for the Exploration Launch Office, within the Constellation Program. A summary of the programmatic drivers which influenced the approach along with details of the resulting implementation will be discussed as well as metrics evaluating the efficiency and accuracy of the various requirements development activities. Requirements development activities will focus on the procedures utilized to ensure that technical content was valid and mature in preparation for the Crew Launch Vehicle and Constellation System s Requirements Reviews. This discussion will begin at initial requirements development during the Exploration Systems Architecture Study and progress through formal development of the program structure. Specific emphasis will be given to development and validation of the requirements. This discussion will focus on approaches to garner the appropriate requirement owners (or customers), project infrastructure utilized to emphasize proper integration, and finally the procedure to technically mature, verify and validate the requirements. Examples of requirements being implemented on the Launch Vehicle (systems, interfaces, test & verification) will be utilized to demonstrate the various processes and also provide a top level understanding of the launch vehicle(s) performance goals. Details may also be provided on the approaches for verification, which range from typical aerospace hardware development (qualification/acceptance) through flight certification (flight test, etc.). The primary intent of this paper is to provide a demonstrated procedure for the development of a mature, effective, integrated set of requirements on a complex system, which also has the added intricacies of both heritage and new hardware development integration. Ancillary focus of the paper will include discussion of Test and Verification approaches along with top level systems/elements performance capabilities.

Differential requirement of PKC-θ in the development and function of Natural Regulatory T cells

PubMed Central

Gupta, Sonal; Manicassamy, Santhakumar; Vasu, Chenthamarakshan; Kumar, Anvita; Shang, Weirong; Sun, Zuoming

2008-01-01

CD4+CD25+ natural Treg cells, which are developed in the thymus, migrate to the periphery to actively maintain self-tolerance. Similar to conventional T cells, TCR signals are critical for the development and activation of Treg cell inhibitory function. While PKC-θ-mediated TCR signals are required for the activation of peripheral naïve T cells, they are dispensable for their thymic development. Here, we show that mice deficient in PKC-θ had a greatly reduced number of CD4+Foxp3+ Treg cells, which was independent of PKC-θ-regulated survival, as transgenic Bcl-xL could not restore the Treg cell population in PKC-θ−/− mice. Active and WT PKC-θ markedly stimulated, whereas inactive PKC-θ and dominant negative NFAT inhibited Foxp3 promoter activity. In addition, mice-deficient in calcineurin Aβ had a decreased Treg cell population, similar to that observed in PKC-θ deficient mice. It is likely that PKC-θ promoted the development of Treg cells by enhancing Foxp3 expression via activation of the calcineurin/NFAT pathway. Finally, Treg cells deficient in PKC-θ were as potent as WT Treg cells in inhibiting T cell activation, indicating that PKC-θ was not required for Treg cell-mediated inhibitory function. Our data highlight the contrasting roles PKC-θ plays in conventional T cell and natural Treg cell function. PMID:18842300

24 CFR 1000.136 - What insurance requirements apply to housing units assisted with NAHASDA grants?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false What insurance requirements apply... ACTIVITIES Affordable Housing Activities § 1000.136 What insurance requirements apply to housing units assisted with NAHASDA grants? (a) The recipient shall provide adequate insurance either by purchasing...

Managing System of Systems Requirements with a Requirements Screening Group

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ronald R. Barden

2012-07-01

Figuring out an effective and efficient way to manage not only your Requirement’s Baseline, but also the development of all your individual requirements during a Program’s/Project’s Conceptual and Development Life Cycle Stages can be both daunting and difficult. This is especially so when you are dealing with a complex and large System of Systems (SoS) Program with potentially thousands and thousands of Top Level Requirements as well as an equal number of lower level System, Subsystem and Configuration Item requirements that need to be managed. This task is made even more overwhelming when you have to add in integration withmore » multiple requirements’ development teams (e.g., Integrated Product Development Teams (IPTs)) and/or numerous System/Subsystem Design Teams. One solution for tackling this difficult activity on a recent large System of Systems Program was to develop and make use of a Requirements Screening Group (RSG). This group is essentially a Team made up of co-chairs from the various Stakeholders with an interest in the Program of record that are enabled and accountable for Requirements Development on the Program/Project. The RSG co-chairs, often with the help of individual support team, work together as a Program Board to monitor, make decisions on, and provide guidance on all Requirements Development activities during the Conceptual and Development Life Cycle Stages of a Program/Project. In addition, the RSG can establish and maintain the Requirements Baseline, monitor and enforce requirements traceability across the entire Program, and work with other elements of the Program/Project to ensure integration and coordination.« less

Progress in Materials and Component Development for Advanced Lithium-ion Cells for NASA's Exploration Missions

NASA Technical Reports Server (NTRS)

Reid, Concha, M.; Reid, Concha M.

2011-01-01

Vehicles and stand-alone power systems that enable the next generation of human missions to the Moon will require energy storage systems that are safer, lighter, and more compact than current state-of-the- art (SOA) aerospace quality lithium-ion (Li-ion) batteries. NASA is developing advanced Li-ion cells to enable or enhance the power systems for the Altair Lunar Lander, Extravehicular Activities spacesuit, and rovers and portable utility pallets for Lunar Surface Systems. Advanced, high-performing materials are required to provide component-level performance that can offer the required gains at the integrated cell level. Although there is still a significant amount of work yet to be done, the present state of development activities has resulted in the synthesis of promising materials that approach the ultimate performance goals. This report on interim progress of the development efforts will elaborate on the challenges of the development activities, proposed strategies to overcome technical issues, and present performance of materials and cell components.

Atmospheric density models

NASA Technical Reports Server (NTRS)

Mueller, A. C.

1977-01-01

An atmospheric model developed by Jacchia, quite accurate but requiring a large amount of computer storage and execution time, was found to be ill-suited for the space shuttle onboard program. The development of a simple atmospheric density model to simulate the Jacchia model was studied. Required characteristics including variation with solar activity, diurnal variation, variation with geomagnetic activity, semiannual variation, and variation with height were met by the new atmospheric density model.

24 CFR 1000.12 - What nondiscrimination requirements are applicable?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false What nondiscrimination requirements are applicable? 1000.12 Section 1000.12 Housing and Urban Development Regulations Relating to Housing..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES General § 1000.12 What...

24 CFR 1000.18 - What environmental review requirements apply?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false What environmental review requirements apply? 1000.18 Section 1000.18 Housing and Urban Development Regulations Relating to Housing and... HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES General § 1000.18 What environmental...

Project Quality Assurance Plan for research and development services provided by Oak Ridge National Laboratory in support of the Westinghouse Materials Company of Ohio Operable Unit 1 Stabilization Development and Treatability Studies Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gilliam, T.M.

1991-05-01

This Project Quality Assurance Plan (PQAP) sets forth the quality assurance (QA) requirements that are applied to those elements of the Westinghouse Materials Company of Ohio (WMCO) Operable Unit 1 support at Oak Ridge National Laboratory (ORNL) project that involve research and development (R D) performed at ORNL. This is in compliance with the applicable criteria of 10 CFR Part 50, Appendix B, ANSI/ASME NQA-1, as specified by Department of Energy (DOE) Oak Ridge Operations (ORO) Order 5700.6B. For this application, NQA-1 is the core QA Program requirements document. QA policy, normally found in the requirements document, is contained herein.more » The requirements of this PQAP apply to project activities that affect the quality and reliability/credibility of research, development, and investigative data and documentation. These activities include the functions of attaining quality objectives and assuring that an appropriate QA program scope is established. The scope of activities affecting quality includes organization; personnel training and qualifications; design control; procurement; material handling and storage; operating procedures; testing, surveillance, and auditing; R D investigative activities and documentation; deficiencies; corrective actions; and QA record keeping. 12 figs.« less

Earth orbital experiment program and requirements study, volume 1, sections 1 - 6

NASA Technical Reports Server (NTRS)

1971-01-01

A reference manual for planners of manned earth-orbital research activity is presented. The manual serves as a systems approach to experiment and mission planning based on an integrated consideration of candidate research programs and the appropriate vehicle, mission, and technology development requirements. Long range goals and objectives for NASA activities during the 1970 to 1980 time period are analyzed. The useful and proper roles of manned and automated spacecraft for implementing NASA experiments are described. An integrated consideration of NASA long range goals and objectives, the system and mission requirements, and the alternative implementation plans are developed. Specific areas of investigation are: (1) manned space flight requirements, (2) space biology, (3) spaceborne astronomy, (4) space communications and navigation, (5) earth observation, (6) supporting technology development requirements, (7) data management system matrices, (8) instrumentation matrices, and (9) biotechnology laboratory experiments.

24 CFR 1000.38 - What flood insurance requirements are applicable?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false What flood insurance requirements..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES General § 1000.38 What flood insurance requirements are applicable? Under the Flood Disaster Protection Act of 1973, as amended (42 U.S.C...

Using Common Graphics Paradigms Implemented in a Java Applet to Represent Complex Scheduling Requirements

NASA Technical Reports Server (NTRS)

Jaap, John; Meyer, Patrick; Davis, Elizabeth

1997-01-01

The experiments planned for the International Space Station promise to be complex, lengthy and diverse. The scarcity of the space station resources will cause significant competition for resources between experiments. The scheduling job facing the Space Station mission planning software requires a concise and comprehensive description of the experiments' requirements (to ensure a valid schedule) and a good description of the experiments' flexibility (to effectively utilize available resources). In addition, the continuous operation of the station, the wide geographic dispersion of station users, and the budgetary pressure to reduce operations manpower make a low-cost solution mandatory. A graphical representation of the scheduling requirements for station payloads implemented via an Internet-based application promises to be an elegant solution that addresses all of these issues. The graphical representation of experiment requirements permits a station user to describe his experiment by defining "activities" and "sequences of activities". Activities define the resource requirements (with alternatives) and other quantitative constraints of tasks to be performed. Activities definitions use an "outline" graphics paradigm. Sequences define the time relationships between activities. Sequences may also define time relationships with activities of other payloads or space station systems. Sequences of activities are described by a "network" graphics paradigm. The bulk of this paper will describe the graphical approach to representing requirements and provide examples that show the ease and clarity with which complex requirements can be represented. A Java applet, to run in a web browser, is being developed to support the graphical representation of payload scheduling requirements. Implementing the entry and editing of requirements via the web solves the problems introduced by the geographic dispersion of users. Reducing manpower is accomplished by developing a concise representation which eliminates the misunderstanding possible with verbose representations and which captures the complete requirements and flexibility of the experiments.

Advanced extravehicular activity systems requirements definition study

NASA Technical Reports Server (NTRS)

1988-01-01

A study to define the requirements for advanced extravehicular activities (AEVA) was conducted. The purpose of the study was to develop an understanding of the EVA technology requirements and to map a pathway from existing or developing technologies to an AEVA system capable of supporting long-duration missions on the lunar surface. The parameters of an AEVA system which must sustain the crewmembers and permit productive work for long periods in the lunar environment were examined. A design reference mission (DRM) was formulated and used as a tool to develop and analyze the EVA systems technology aspects. Many operational and infrastructure design issues which have a significant influence on the EVA system are identified.

Microgravity Fluid Management Symposium

NASA Technical Reports Server (NTRS)

1987-01-01

The NASA Microgravity Fluid Management Symposium, held at the NASA Lewis Research Center, September 9 to 10, 1986, focused on future research in the microgravity fluid management field. The symposium allowed researchers and managers to review space applications that require fluid management technology, to present the current status of technology development, and to identify the technology developments required for future missions. The 19 papers covered three major categories: (1) fluid storage, acquisition, and transfer; (2) fluid management applications, i.e., space power and thermal management systems, and environmental control and life support systems; (3) project activities and insights including two descriptions of previous flight experiments and a summary of typical activities required during development of a shuttle flight experiment.

24 CFR 1000.46 - Do drug-free workplace requirements apply?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Do drug-free workplace requirements apply? 1000.46 Section 1000.46 Housing and Urban Development Regulations Relating to Housing and Urban... URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES General § 1000.46 Do drug-free workplace...

Spaceflight Radiation Health program at the Lyndon B. Johnson Space Center

NASA Technical Reports Server (NTRS)

Johnson, A. Steve; Badhwar, Gautam D.; Golightly, Michael J.; Hardy, Alva C.; Konradi, Andrei; Yang, Tracy Chui-Hsu

1993-01-01

The Johnson Space Center leads the research and development activities that address the health effects of space radiation exposure to astronaut crews. Increased knowledge of the composition of the environment and of the biological effects of space radiation is required to assess health risks to astronaut crews. The activities at the Johnson Space Center range from quantification of astronaut exposures to fundamental research into the biological effects resulting from exposure to high energy particle radiation. The Spaceflight Radiation Health Program seeks to balance the requirements for operational flexibility with the requirement to minimize crew radiation exposures. The components of the space radiation environment are characterized. Current and future radiation monitoring instrumentation is described. Radiation health risk activities are described for current Shuttle operations and for research development program activities to shape future analysis of health risk.

Tried and True: The Romance of the Atoms--Animated Atomic Attractions

ERIC Educational Resources Information Center

Hibbitt, Catherine

2010-01-01

Since the formation of atomic bonds is active, the authors sought a way of learning through drama or kinetic activity. To achieve this goal, they developed an activity called Romance of the Atoms. The activity requires students to use computer-animation technology to develop short cartoons that explain atomic classification and bonds. This…

NASA safety program activities in support of the Space Exploration Initiatives Nuclear Propulsion program

NASA Technical Reports Server (NTRS)

Sawyer, J. C., Jr.

1993-01-01

The activities of the joint NASA/DOE/DOD Nuclear Propulsion Program Technical Panels have been used as the basis for the current development of safety policies and requirements for the Space Exploration Initiatives (SEI) Nuclear Propulsion Technology development program. The Safety Division of the NASA Office of Safety and Mission Quality has initiated efforts to develop policies for the safe use of nuclear propulsion in space through involvement in the joint agency Nuclear Safety Policy Working Group (NSPWG), encouraged expansion of the initial policy development into proposed programmatic requirements, and suggested further expansion into the overall risk assessment and risk management process for the NASA Exploration Program. Similar efforts are underway within the Department of Energy to ensure the safe development and testing of nuclear propulsion systems on Earth. This paper describes the NASA safety policy related to requirements for the design of systems that may operate where Earth re-entry is a possibility. The expected plan of action is to support and oversee activities related to the technology development of nuclear propulsion in space, and support the overall safety and risk management program being developed for the NASA Exploration Program.

24 CFR 598.400 - HUD grants for planning activities.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 3 2012-04-01 2012-04-01 false HUD grants for planning activities. 598.400 Section 598.400 Housing and Urban Development Regulations Relating to Housing and Urban... DESIGNATIONS Post-Designation Requirements § 598.400 HUD grants for planning activities. (a) HUD will award...

24 CFR 598.400 - HUD grants for planning activities.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 3 2014-04-01 2013-04-01 true HUD grants for planning activities. 598.400 Section 598.400 Housing and Urban Development Regulations Relating to Housing and Urban... DESIGNATIONS Post-Designation Requirements § 598.400 HUD grants for planning activities. (a) HUD will award...

24 CFR 598.400 - HUD grants for planning activities.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false HUD grants for planning activities. 598.400 Section 598.400 Housing and Urban Development Regulations Relating to Housing and Urban... DESIGNATIONS Post-Designation Requirements § 598.400 HUD grants for planning activities. (a) HUD will award...

77 FR 9237 - Agency Information Collection Activities; Proposed Collection; Comment Request; Risk Management...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-16

... Activities; Proposed Collection; Comment Request; Risk Management Program Requirements and Petitions To..., non-chemical manufacturers, etc. Title: Risk Management Program Requirements and Petitions to Modify... regulated substance in a process develop and implement a risk management program and submit a risk...

Team-Based Professional Development Interventions in Higher Education: A Systematic Review

ERIC Educational Resources Information Center

Gast, Inken; Schildkamp, Kim; van der Veen, Jan T.

2017-01-01

Most professional development activities focus on individual teachers, such as mentoring or the use of portfolios. However, new developments in higher education require teachers to work together in teams more often. Due to these changes, there is a growing need for professional development activities focusing on teams. Therefore, this review study…

24 CFR 598.400 - HUD grants for planning activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... process describes the procedures for award of these planning grants, post-award reporting requirements... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false HUD grants for planning activities... Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF...

30 CFR 550.250 - What oil and hazardous substance spills information must accompany the DPP or DOCD?

Code of Federal Regulations, 2014 CFR

2014-07-01

... SHELF Plans and Information Contents of Development and Production Plans (dpp) and Development... accompany your DPP or DOCD: (a) Oil spill response planning. The material required under paragraph (a)(1) or... conduct your proposed development and production activities prepared according to the requirements of 30...

30 CFR 550.250 - What oil and hazardous substance spills information must accompany the DPP or DOCD?

Code of Federal Regulations, 2013 CFR

2013-07-01

... SHELF Plans and Information Contents of Development and Production Plans (dpp) and Development... accompany your DPP or DOCD: (a) Oil spill response planning. The material required under paragraph (a)(1) or... conduct your proposed development and production activities prepared according to the requirements of 30...

30 CFR 550.250 - What oil and hazardous substance spills information must accompany the DPP or DOCD?

Code of Federal Regulations, 2012 CFR

2012-07-01

... SHELF Plans and Information Contents of Development and Production Plans (dpp) and Development... accompany your DPP or DOCD: (a) Oil spill response planning. The material required under paragraph (a)(1) or... conduct your proposed development and production activities prepared according to the requirements of 30...

E2f1–3 Are Critical for Myeloid Development*

PubMed Central

Trikha, Prashant; Sharma, Nidhi; Opavsky, Rene; Reyes, Andres; Pena, Clarissa; Ostrowski, Michael C.; Roussel, Martine F.; Leone, Gustavo

2011-01-01

Hematopoietic development involves the coordinated activity of differentiation and cell cycle regulators. In current models of mammalian cell cycle control, E2f activators (E2f1, E2f2, and E2f3) are portrayed as the ultimate transcriptional effectors that commit cells to enter and progress through S phase. Using conditional gene knock-out strategies, we show that E2f1–3 are not required for the proliferation of early myeloid progenitors. Rather, these E2fs are critical for cell survival and proliferation at two distinct steps of myeloid development. First, E2f1–3 are required as transcriptional repressors for the survival of CD11b+ myeloid progenitors, and then they are required as activators for the proliferation of CD11b+ macrophages. In bone marrow macrophages, we show that E2f1–3 respond to CSF1-Myc mitogenic signals and serve to activate E2f target genes and promote their proliferation. Together, these findings expose dual functions for E2f1–3 at distinct stages of myeloid development in vivo, first as repressors in cell survival and then as activators in cell proliferation. In summary, this work places E2f1–3 in a specific signaling cascade that is critical for myeloid development in vivo. PMID:21115501

A rho-binding protein kinase C-like activity is required for the function of protein kinase N in Drosophila development.

PubMed

Betson, Martha; Settleman, Jeffrey

2007-08-01

The Rho GTPases interact with multiple downstream effectors to exert their biological functions, which include important roles in tissue morphogenesis during the development of multicellular organisms. Among the Rho effectors are the protein kinase N (PKN) proteins, which are protein kinase C (PKC)-like kinases that bind activated Rho GTPases. The PKN proteins are well conserved evolutionarily, but their biological role in any organism is poorly understood. We previously determined that the single Drosophila ortholog of mammalian PKN proteins, Pkn, is a Rho/Rac-binding kinase essential for Drosophila development. By performing "rescue" studies with various Pkn mutant constructs, we have defined the domains of Pkn required for its role during Drosophila development. These studies suggested that Rho, but not Rac binding is important for Pkn function in development. In addition, we determined that the kinase domain of PKC53E, a PKC family kinase, can functionally substitute for the kinase domain of Pkn during development, thereby exemplifying the evolutionary strategy of "combining" functional domains to produce proteins with distinct biological activities. Interestingly, we also identified a requirement for Pkn in wing morphogenesis, thereby revealing the first postembryonic function for Pkn.

77 FR 51528 - Notice of Public Hearings for the Draft Environmental Impact Statement for Outdoor Research...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-24

... Impact Statement for Outdoor Research, Development, Test and Evaluation Activities, Naval Surface Warfare..., development, test and evaluation (RDT&E) activities within the Potomac River Test Range (PRTR) complex... current, baseline RDT&E mission requirements for activities that have the potential to affect human health...

Top 10 Research Questions Related to Children Physical Activity Motivation

ERIC Educational Resources Information Center

Chen, Ang

2013-01-01

Physical activity is critical to healthy development of children. It is well documented that helping children develop and sustain a physically active lifestyle requires children to become motivated. Many studies have been conducted in the past 2.5 decades on determinants and correlates for children and adolescents' physical activity…

Activity Theory in Information Systems Research and Practice: Theoretical Underpinnings for an Information Systems Development Model

ERIC Educational Resources Information Center

Mursu, Anja; Luukkonen, Irmeli; Toivanen, Marika; Korpela, Mikko

2007-01-01

Introduction: The purpose of information systems is to facilitate work activities: here we consider how Activity Theory can be applied in information systems development. Method. The requirements for an analytical model for emancipatory, work-oriented information systems research and practice are specified. Previous research work in Activity…

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation

PubMed Central

Yang, Meixiang; Chen, Shasha; Du, Juan; He, Junming; Wang, Yuande; Li, Zehua; Liu, Guangao; Peng, Wanwen; Zeng, Xiaokang; Li, Dan; Xu, Panglian; Guo, Wei; Chang, Zai; Wang, Song; Tian, Zhigang; Dong, Zhongjun

2016-01-01

Activation of metabolic signalling by IL-15 is required for natural killer (NK) cell development. Here we show that Tsc1, a repressor of mTOR, is dispensable for the terminal maturation, survival and function of NK cells but is critical to restrict exhaustive proliferation of immature NK cells and activation downstream of IL-15 during NK cell development. Tsc1 is expressed in immature NK cells and is upregulated by IL-15. Haematopoietic-specific deletion of Tsc1 causes a marked decrease in the number of NK cells and compromises rejection of ‘missing-self' haematopoietic tumours and allogeneic bone marrow. The residual Tsc1-null NK cells display activated, pro-apoptotic phenotype and elevated mTORC1 activity. Deletion of Raptor, a component of mTORC1, largely reverses these defects. Tsc1-deficient NK cells express increased levels of T-bet and downregulate Eomes and CD122, a subunit of IL-15 receptor. These results reveal a role for Tsc1-dependent inhibition of mTORC1 activation during immature NK cell development. PMID:27601261

On-orbit Metrology and Calibration Requirements for Space Station Activities Definition Study

NASA Technical Reports Server (NTRS)

Cotty, G. M.; Ranganathan, B. N.; Sorrell, A. L.

1989-01-01

The Space Station is the focal point for the commercial development of space. The long term routine operation of the Space Station and the conduct of future commercial activities suggests the need for in-space metrology capabilities analogous when possible to those on-Earth. The ability to perform periodic calibrations and measurements with proper traceability is imperative for the routine operation of the Space Station. An initial review, however, indicated a paucity of data related to metrology and calibration requirements for in-space operations. This condition probably exists because of the highly developmental aspect of space activities to date, their short duration, and nonroutine nature. The on-orbit metrology and calibration needs of the Space Station were examined and assessed. In order to achieve this goal, the following tasks were performed: an up-to-date literature review; identification of on-orbit calibration techniques; identification of sensor calibration requirements; identification of calibration equipment requirements; definition of traceability requirements; preparation of technology development plans; and preparation of the final report. Significant information and major highlights pertaining to each task is presented. In addition, some general (generic) conclusions/observations and recommendations that are pertinent to the overall in-space metrology and calibration activities are presented.

NASA Procurement Career Development Program

NASA Technical Reports Server (NTRS)

1987-01-01

The NASA Procurement Career Development Program establishes an agency-wide framework for the management of career development activity in the procurement field. Within this framework, installations are encouraged to modify the various components to meet installation-specific mission and organization requirements. This program provides a systematic process for the assessment of and planning for the development, training, and education required to increase the employees' competence in the procurement work functions. It includes the agency-wide basic knowledge and skills by career field and level upon which individual and organizational development plans are developed. Also, it provides a system that is compatible with other human resource management and development systems, processes, and activities. The compatibility and linkage are important in fostering the dual responsibility of the individual and the organization in the career development process.

78 FR 37819 - Proposed Information Collection Activity; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-24

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Administration for Children and Families Proposed Information Collection Activity; Comment Request Proposed Projects Title: Child Care and Development Block Grant Reporting Requirements--ACF-700. OMB No.: 0970-0430. Description: Thee Child Care and Development...

75 FR 30031 - Proposed Information Collection Activity; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-28

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Administration for Children and Families Proposed Information Collection Activity; Comment Request Proposed Projects Title: Child Care and Development Block Grant Reporting Requirements--ACF-700. OMB No.: 0980-0241. Description: The Child Care and Development...

Space Station Human Factors Research Review. Volume 1: EVA Research and Development

NASA Technical Reports Server (NTRS)

Cohen, Marc M. (Editor); Vykukal, H. C. (Editor)

1988-01-01

An overview is presented of extravehicular activity (EVA) research and development activities at Ames. The majority of the program was devoted to presentations by the three contractors working in parallel on the EVA System Phase A Study, focusing on Implications for Man-Systems Design. Overhead visuals are included for a mission results summary, space station EVA requirements and interface accommodations summary, human productivity study cross-task coordination, and advanced EVAS Phase A study implications for man-systems design. Articles are also included on subsea approach to work systems development and advanced EVA system design requirements.

Configuration management plan. System definition and project development. Repository Based Software Engineering (RBSE) program

NASA Technical Reports Server (NTRS)

Mckay, Charles

1991-01-01

This is the configuration management Plan for the AdaNet Repository Based Software Engineering (RBSE) contract. This document establishes the requirements and activities needed to ensure that the products developed for the AdaNet RBSE contract are accurately identified, that proposed changes to the product are systematically evaluated and controlled, that the status of all change activity is known at all times, and that the product achieves its functional performance requirements and is accurately documented.

[The present status and development of thermal control system of spacesuits for extravehicular activity].

PubMed

Zhao, C Y; Sun, J B; Yuan, X G

1999-04-01

With the extension of extravehicular activity (EVA) duration, the need for more effective thermal control of EVA spacesuits is required. The specific schemes investigated in heat sink system for EVA are discussed, including radiator, ice storage, metal hydride heat pump, phase-change storage/radiator and sublimator. The importance and requirements of automatic thermal control for EVA are also discussed. Existed automatic thermal control for EVA are reviewed. Prospects of further developments of thermal control of spacesuits for EVA are proposed.

Formulation of detailed consumables management models for the development (preoperational) period of advanced space transportation system. Volume 2: Consumables data base workbook

NASA Technical Reports Server (NTRS)

Zamora, M. A.

1976-01-01

The consumables characteristic data associated with the performance of the mission activities required by the mission planning processor are defined to calculate the consumables requirements. The activity data is defined in terms of discrete time periods having a distinct rate for each consumable required to support the performance of a given operation. The data is structured in a series of consumable data worksheets for each activity that includes a profile of its operations and the rate of each consumable required to support the given activity. The data worksheets provide for the uniform specification of consumables data, allows for the ready identification of the consumables affected by a given activity, and facilitates the updating process. An activity and the data that must be included in the data worksheets are defined and an example of its use and application the consumables data requirements for the performance of the EVA are presented.

77 FR 31864 - Prospective Grant of Exclusive License: Development of PANVACTM

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-30

... costimulatory molecule when used individually to optimally activate both CD4+ and CD8+ T cells. When a TRICOM... of an effective T-cell immune response to antigens requires two signals. The first one is antigen... required for cytokine production, proliferation, and other aspects of T-cell activation. The patents and...

Brain Stretchers, Book 1.

ERIC Educational Resources Information Center

Anderson, Carolyn; Haller, Jackie

This collection of activities is designed to help students develop critical thinking skills. The activities are non-graded and can be used from upper elementary to high school. Reading levels vary from no reading required to very little reading required and can be used effectively with students who are poor readers, bilingual, or at a special…

Brain Stretchers, Book 2.

ERIC Educational Resources Information Center

Anderson, Carolyn; Haller, Jackie

This collection of activities is designed to help students develop critical thinking skills. The activities are non-graded and can be used from upper elementary to high school. The reading levels vary from no reading required to very little reading required and can be used effectively with students who are poor readers, bilingual, or at a special…

LANL surveillance requirements management and surveillance requirements from NA-12 tasking memo

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hills, Charles R

2011-01-25

Surveillance briefing to NNSA to support a tasking memo from NA-12 on Surveillance requirements. This talk presents the process for developing surveillance requirements, discusses the LANL requirements that were issued as part of that tasking memo, and presents recommendations on Component Evaluation and Planning Committee activities for FY11.

An active role for machine learning in drug development

PubMed Central

Murphy, Robert F.

2014-01-01

Due to the complexity of biological systems, cutting-edge machine-learning methods will be critical for future drug development. In particular, machine-vision methods to extract detailed information from imaging assays and active-learning methods to guide experimentation will be required to overcome the dimensionality problem in drug development. PMID:21587249

Enhancing International Research and Development-Project Activity on University Campuses: Insights from U.S. Senior International Officers

ERIC Educational Resources Information Center

Koehn, Peter H.; Deardorff, Darla K.; Bolognese, Kerry D.

2011-01-01

In the interconnected world of the UN Decade of Education for Sustainable Development, the ability of higher-education institutions to contribute to and benefit from international research undertakings, sustainable-development-project activity, and capacity-building endeavors requires transnational involvement. While the potential benefits are…

Development of a teen-focused exergame

USDA-ARS?s Scientific Manuscript database

Exergames require body movement to play and may be an effective method for enhancing teen physical activity. However, results have been mixed. Innovative methods are needed to develop exergames that increase and maintain physical activity. Self-representational avatars, or avatars created from a dig...

Data quality objectives for TWRS privatization phase 1: confirm tank T is an appropriate feed source for low-activity waste feed batch X

DOE Office of Scientific and Technical Information (OSTI.GOV)

NGUYEN, D.M.

1999-06-01

The US. Department of Energy, Richland Operations Office (DOE-RL) has initiated Phase 1 of a two-phase privatization strategy for treatment and immobilization of low-activity waste (LAW) currently being managed by the Hanford Tank Waste Remediation System (TWRS) Project. In this strategy, DOE will purchase services from a contractor-owned and operated facility under a fixed price. The Phase 1 TWRS privatization contract requires that the Project Hanford Management Contract (PHMC) contractors, on behalf of DOE, deliver LAW feed in specified quantities and composition to the Privatization Contractor in a timely manner (DOE-RL 1996). Additional requirements are imposed by the interface controlmore » document (ICD-19) for LAW feed (PHMC 1997). In response to these requirements, the Tank Waste Remediation System Operation and Utilization Plan (TWRSO and UP) (Kirkbride et al. 1997) was prepared by the PHMC. The TWRSO and UP, as updated by the Readiness-To-Proceed deliverable (Payne et al. 1998), establishes the baseline operating scenario for the delivery of LAW feed to the Privatization Contractor. The scenario specifies tanks from which LAW will be provided for each feed batch, the operational activities needed to prepare and deliver each batch, and the timing of these activities. The operating scenario was developed based on current knowledge of waste composition and chemistry, waste transfer methods, and operating constraints, such as tank farm logistics and availability of tank space. A project master baseline schedule (PMBS) has been developed to implement the operating scenario. The PMBS also includes activities aimed at reducing programmatic risks. One of the activities, ''Confirm Plans and Requirements,'' was identified to verify the basis used to develop the scenario. Additional data on waste quantity, physical and chemical characteristics, and transfer properties will be needed to support this activity. This document describes the data quality objective (DQO) process undertaken to assme appropriate data will be collected to support the activity, ''Confirm Tank Plans and Requirements.'' The DQO process was implemented in accordance with the TWRS DQO process (Banning 1997) with some modifications to accommodate project or tank-specific requirements and constraints.« less

In-space propellant logistics. Volume 4: Project planning data

NASA Technical Reports Server (NTRS)

1972-01-01

The prephase A conceptual project planning data as it pertains to the development of the selected logistics module configuration transported into earth orbit by the space shuttle orbiter. The data represents the test, implementation, and supporting research and technology requirements for attaining the propellant transfer operational capability for early 1985. The plan is based on a propellant module designed to support the space-based tug with cryogenic oxygen-hydrogen propellants. A logical sequence of activities that is required to define, design, develop, fabricate, test, launch, and flight test the propellant logistics module is described. Included are the facility and ground support equipment requirements. The schedule of activities are based on the evolution and relationship between the R and T, the development issues, and the resultant test program.

Senseless, a Zn finger transcription factor, is necessary and sufficient for sensory organ development in Drosophila

NASA Technical Reports Server (NTRS)

Nolo, R.; Abbott, L. A.; Bellen, H. J.

2000-01-01

The senseless (sens) gene is required for proper development of most cell types of the embryonic and adult peripheral nervous system (PNS) of Drosophila. Sens is a nuclear protein with four Zn fingers that is expressed and required in the sensory organ precursors (SOP) for proper proneural gene expression. Ectopic expression of Sens in many ectodermal cells causes induction of PNS external sensory organ formation and is able to recreate an ectopic proneural field. Hence, sens is both necessary and sufficient for PNS development. Our data indicate that proneural genes activate sens expression. Sens is then in turn required to further activate and maintain proneural gene expression. This feedback mechanism is essential for selective enhancement and maintenance of proneural gene expression in the SOPs.

Comparative Metabolomic Profiling Reveals That Dysregulated Glycolysis Stemming from Lack of Salvage NAD+ Biosynthesis Impairs Reproductive Development in Caenorhabditis elegans.

PubMed

Wang, Wenqing; McReynolds, Melanie R; Goncalves, Jimmy F; Shu, Muya; Dhondt, Ineke; Braeckman, Bart P; Lange, Stephanie E; Kho, Kelvin; Detwiler, Ariana C; Pacella, Marisa J; Hanna-Rose, Wendy

2015-10-23

Temporal developmental progression is highly coordinated in Caenorhabditis elegans. However, loss of nicotinamidase PNC-1 activity slows reproductive development, uncoupling it from its typical progression relative to the soma. Using LC/MS we demonstrate that pnc-1 mutants do not salvage the nicotinamide released by NAD(+) consumers to resynthesize NAD(+), resulting in a reduction in global NAD(+) bioavailability. We manipulate NAD(+) levels to demonstrate that a minor deficit in NAD(+) availability is incompatible with a normal pace of gonad development. The NAD(+) deficit compromises NAD(+) consumer activity, but we surprisingly found no functional link between consumer activity and reproductive development. As a result we turned to a comparative metabolomics approach to identify the cause of the developmental phenotype. We reveal widespread metabolic perturbations, and using complementary pharmacological and genetic approaches, we demonstrate that a glycolytic block accounts for the slow pace of reproductive development. Interestingly, mitochondria are protected from both the deficiency in NAD(+) biosynthesis and the effects of reduced glycolytic output. We suggest that compensatory metabolic processes that maintain mitochondrial activity in the absence of efficient glycolysis are incompatible with the requirements for reproductive development, which requires high levels of cell division. In addition to demonstrating metabolic requirements for reproductive development, this work also has implications for understanding the mechanisms behind therapeutic interventions that target NAD(+) salvage biosynthesis for the purposes of inhibiting tumor growth. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Comparative Metabolomic Profiling Reveals That Dysregulated Glycolysis Stemming from Lack of Salvage NAD+ Biosynthesis Impairs Reproductive Development in Caenorhabditis elegans*

PubMed Central

Wang, Wenqing; McReynolds, Melanie R.; Goncalves, Jimmy F.; Shu, Muya; Dhondt, Ineke; Braeckman, Bart P.; Lange, Stephanie E.; Kho, Kelvin; Detwiler, Ariana C.; Pacella, Marisa J.; Hanna-Rose, Wendy

2015-01-01

Temporal developmental progression is highly coordinated in Caenorhabditis elegans. However, loss of nicotinamidase PNC-1 activity slows reproductive development, uncoupling it from its typical progression relative to the soma. Using LC/MS we demonstrate that pnc-1 mutants do not salvage the nicotinamide released by NAD+ consumers to resynthesize NAD+, resulting in a reduction in global NAD+ bioavailability. We manipulate NAD+ levels to demonstrate that a minor deficit in NAD+ availability is incompatible with a normal pace of gonad development. The NAD+ deficit compromises NAD+ consumer activity, but we surprisingly found no functional link between consumer activity and reproductive development. As a result we turned to a comparative metabolomics approach to identify the cause of the developmental phenotype. We reveal widespread metabolic perturbations, and using complementary pharmacological and genetic approaches, we demonstrate that a glycolytic block accounts for the slow pace of reproductive development. Interestingly, mitochondria are protected from both the deficiency in NAD+ biosynthesis and the effects of reduced glycolytic output. We suggest that compensatory metabolic processes that maintain mitochondrial activity in the absence of efficient glycolysis are incompatible with the requirements for reproductive development, which requires high levels of cell division. In addition to demonstrating metabolic requirements for reproductive development, this work also has implications for understanding the mechanisms behind therapeutic interventions that target NAD+ salvage biosynthesis for the purposes of inhibiting tumor growth. PMID:26350462

Using the Internet to Educate the World: The "Earthlearningidea" Website

ERIC Educational Resources Information Center

Kennett, Peter

2012-01-01

The website "Earthlearningidea" was developed for the International Year of Planet Earth in 2007 and continues with one new activity per fortnight. Activities are devised to catch the attention of students and to enable them to enjoy the science of the Earth. Many activities require minimal equipment, for schools in developing countries, but there…

How to … be an active appraiser.

PubMed

Austin, Trevor

2013-07-01

Being an active appraiser is very challenging in terms of knowing a doctor's work well enough to appraise them effectively. It also requires a set of skills that need to be developed and applied well in a complex system and in the context of an individual doctor's career development. Being 'active' means bringing these skills and principles together.

Parabolic Dish Solar Thermal Power Annual Program Review Proceedings

NASA Technical Reports Server (NTRS)

Lucas, J. W.

1982-01-01

The results of activities of the parabolic dish technology and applications development element of DOE's Solar Thermal Energy System Program are presented. Topics include the development and testing of concentrators, receivers, and power conversion units; system design and development for engineering experiments; economic analysis and marketing assessment; and advanced development activities. A panel discussion concerning industrial support sector requirements is also documented.

ATTDES: An Expert System for Satellite Attitude Determination and Control. 2

NASA Technical Reports Server (NTRS)

Mackison, Donald L.; Gifford, Kevin

1996-01-01

The design, analysis, and flight operations of satellite attitude determintion and attitude control systems require extensive mathematical formulations, optimization studies, and computer simulation. This is best done by an analyst with extensive education and experience. The development of programs such as ATTDES permit the use of advanced techniques by those with less experience. Typical tasks include the mission analysis to select stabilization and damping schemes, attitude determination sensors and algorithms, and control system designs to meet program requirements. ATTDES is a system that includes all of these activities, including high fidelity orbit environment models that can be used for preliminary analysis, parameter selection, stabilization schemes, the development of estimators covariance analyses, and optimization, and can support ongoing orbit activities. The modification of existing simulations to model new configurations for these purposes can be an expensive, time consuming activity that becomes a pacing item in the development and operation of such new systems. The use of an integrated tool such as ATTDES significantly reduces the effort and time required for these tasks.

Access 5 - Step 1: Human Systems Integration Program Plan (HSIPP)

NASA Technical Reports Server (NTRS)

2005-01-01

This report describes the Human System Interface (HSI) analysis, design and test activities that will be performed to support the development of requirements and design guidelines to facilitate the incorporation of High Altitude Long Endurance (HALE) Remotely Operated Aircraft (ROA) at or above FL400 in the National Airspace System (NAS). These activities are required to support the design and development of safe, effective and reliable ROA operator and ATC interfaces. This plan focuses on the activities to be completed for Step 1 of the ACCESS 5 program. Updates to this document will be made for each of the four ACCESS 5 program steps.

Strategic service-line planning. Building competitive advantage.

PubMed

Greenspan, Elizabeth; Krentz, Susanna E; O'Neill, Molly K

2003-12-01

Service-line planning requires a healthcare organization to develop a business plan for each of its service lines. Successful service-line planning requires top leadership support, a willingness to allocate resources, the development of support mechanisms, the active support and involvement of physicians, and management commitment and accountability during implementation.

Endodermal Hedgehog signals modulate Notch pathway activity in the developing digestive tract mesenchyme

PubMed Central

Kim, Tae-Hee; Kim, Byeong-Moo; Mao, Junhao; Rowan, Sheldon; Shivdasani, Ramesh A.

2011-01-01

The digestive tract epithelium and its adjoining mesenchyme undergo coordinated patterning and growth during development. The signals they exchange in the process are not fully characterized but include ligands of the Hedgehog (Hh) family, which originate in the epithelium and are necessary for mesenchymal cells to expand in number and drive elongation of the developing gut tube. The Notch signaling pathway has known requirements in fetal and adult intestinal epithelial progenitors. We detected Notch pathway activity in the embryonic gut mesenchyme and used conditional knockout mice to study its function. Selective disruption of the Notch effector gene RBP-Jκ (Rbpj) in the mesenchyme caused progressive loss of subepithelial fibroblasts and abbreviated gut length, revealing an unexpected requirement in this compartment. Surprisingly, constitutive Notch activity also induced rapid mesenchymal cell loss and impaired organogenesis, probably resulting from increased cell death and suggesting the need for a delicate balance in Notch signaling. Because digestive tract anomalies in mouse embryos with excess Notch activity phenocopy the absence of Hh signaling, we postulated that endodermal Hh restrains mesenchymal Notch pathway activity. Indeed, Hh-deficient embryos showed Notch overactivity in their defective gut mesenchyme and exposure to recombinant sonic hedgehog could override Notch-induced death of cultured fetal gut mesenchymal cells. These results reveal unexpected interactions between prominent signals in gastrointestinal development and provide a coherent explanation for Hh requirements in mesenchymal cell survival and organ growth. PMID:21750033

Environmental restoration and waste management: Robotics technology development program: Robotics 5-year program plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

In FY 1990 Robotics Technology Development Program (RTDP) planning teams visited five DOE sites. These sites were selected by the Office of Technology Development to provide a needs basis for developing a 5-Year Plan. Visits to five DOE sites provided identification of needs for robotics technology development to support Environmental Restoration and Waste Management (ER WM) projects at those sites. Additional site visits will be conducted in the future to expand the planning basis. This volume summarizes both the results of the site visits and the needs and requirements of the priority ER WM activities at the sites, including potentialmore » needs for robotics and remote systems technology. It also discusses hazards associated with the site activities and any problems or technical uncertainties associated with dealing with the hazards in the performance of the ER WM work. Robotic or remote systems currently under development for remediation projects or waste operations are also discussed. The information in this document is organized principally by site, activity, and priority. Section 2.0, Site Needs, is based on information from the site visit reports and provides a summary which focuses on the site needs and requirements for each priority activity. Section 2.0 also records evaluations and discussions by the RTDP team following the site visit. Section 3.0, Commonality Assessment, documents similar site needs where common, or cross-cutting, robotics technology might be applied to several activities. Section 4.0 contains a summary of the site needs and requirements in tabular form. 1 tab.« less

Decision Support System Requirements Definition for Human Extravehicular Activity Based on Cognitive Work Analysis

PubMed Central

Miller, Matthew James; McGuire, Kerry M.; Feigh, Karen M.

2016-01-01

The design and adoption of decision support systems within complex work domains is a challenge for cognitive systems engineering (CSE) practitioners, particularly at the onset of project development. This article presents an example of applying CSE techniques to derive design requirements compatible with traditional systems engineering to guide decision support system development. Specifically, it demonstrates the requirements derivation process based on cognitive work analysis for a subset of human spaceflight operations known as extravehicular activity. The results are presented in two phases. First, a work domain analysis revealed a comprehensive set of work functions and constraints that exist in the extravehicular activity work domain. Second, a control task analysis was performed on a subset of the work functions identified by the work domain analysis to articulate the translation of subject matter states of knowledge to high-level decision support system requirements. This work emphasizes an incremental requirements specification process as a critical component of CSE analyses to better situate CSE perspectives within the early phases of traditional systems engineering design. PMID:28491008

Decision Support System Requirements Definition for Human Extravehicular Activity Based on Cognitive Work Analysis.

PubMed

Miller, Matthew James; McGuire, Kerry M; Feigh, Karen M

2017-06-01

The design and adoption of decision support systems within complex work domains is a challenge for cognitive systems engineering (CSE) practitioners, particularly at the onset of project development. This article presents an example of applying CSE techniques to derive design requirements compatible with traditional systems engineering to guide decision support system development. Specifically, it demonstrates the requirements derivation process based on cognitive work analysis for a subset of human spaceflight operations known as extravehicular activity . The results are presented in two phases. First, a work domain analysis revealed a comprehensive set of work functions and constraints that exist in the extravehicular activity work domain. Second, a control task analysis was performed on a subset of the work functions identified by the work domain analysis to articulate the translation of subject matter states of knowledge to high-level decision support system requirements. This work emphasizes an incremental requirements specification process as a critical component of CSE analyses to better situate CSE perspectives within the early phases of traditional systems engineering design.

Light-Activated Content Release from Liposomes

PubMed Central

Leung, Sarah J.; Romanowski, Marek

2012-01-01

Successful integration of diagnostic and therapeutic actions at the level of individual cells requires new materials that combine biological compatibility with functional versatility. This review focuses on the development of liposome-based functional materials, where payload release is activated by light. Methods of sensitizing liposomes to light have progressed from the use of organic molecular moieties to the use of metallic plasmon resonant structures. This development has facilitated application of near infrared light for activation, which is preferred for its deep penetration and low phototoxicity in biological tissues. Presented mechanisms of light-activated liposomal content release enable precise in vitro manipulation of minute amounts of reagents, but their use in clinical diagnostic and therapeutic applications will require demonstration of safety and efficacy. PMID:23139729

Mutant Kras- and p16-regulated NOX4 activation overcomes metabolic checkpoints in development of pancreatic ductal adenocarcinoma

PubMed Central

Ju, Huai-Qiang; Ying, Haoqiang; Tian, Tian; Ling, Jianhua; Fu, Jie; Lu, Yu; Wu, Min; Yang, Lifeng; Achreja, Abhinav; Chen, Gang; Zhuang, Zhuonan; Wang, Huamin; Nagrath, Deepak; Yao, Jun; Hung, Mien-Chie; DePinho, Ronald A.; Huang, Peng; Xu, Rui-Hua; Chiao, Paul J.

2017-01-01

Kras activation and p16 inactivation are required to develop pancreatic ductal adenocarcinoma (PDAC). However, the biochemical mechanisms underlying these double alterations remain unclear. Here we discover that NAD(P)H oxidase 4 (NOX4), an enzyme known to catalyse the oxidation of NAD(P)H, is upregulated when p16 is inactivated by looking at gene expression profiling studies. Activation of NOX4 requires catalytic subunit p22phox, which is upregulated following Kras activation. Both alterations are also detectable in PDAC cell lines and patient specimens. Furthermore, we show that elevated NOX4 activity accelerates oxidation of NADH and supports increased glycolysis by generating NAD+, a substrate for GAPDH-mediated glycolytic reaction, promoting PDAC cell growth. Mechanistically, NOX4 was induced through p16-Rb-regulated E2F and p22phox was induced by KrasG12V-activated NF-κB. In conclusion, we provide a biochemical explanation for the cooperation between p16 inactivation and Kras activation in PDAC development and suggest that NOX4 is a potential therapeutic target for PDAC. PMID:28232723

30 CFR 282.28 - Environmental protection measures.

Code of Federal Regulations, 2012 CFR

2012-07-01

...)(1) The lessee shall monitor activities in a manner that develops the data and information necessary... activities on the environment on and off the lease; develop and evaluate methods for mitigating adverse... with lease and other requirements for the protection of the environment. (2) Monitoring of...

30 CFR 282.28 - Environmental protection measures.

Code of Federal Regulations, 2013 CFR

2013-07-01

...)(1) The lessee shall monitor activities in a manner that develops the data and information necessary... activities on the environment on and off the lease; develop and evaluate methods for mitigating adverse... with lease and other requirements for the protection of the environment. (2) Monitoring of...

30 CFR 282.28 - Environmental protection measures.

Code of Federal Regulations, 2014 CFR

2014-07-01

...)(1) The lessee shall monitor activities in a manner that develops the data and information necessary... activities on the environment on and off the lease; develop and evaluate methods for mitigating adverse... with lease and other requirements for the protection of the environment. (2) Monitoring of...

48 CFR 927.408 - Cosponsored research and development activities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Cosponsored research and... GENERAL CONTRACTING REQUIREMENTS PATENTS, DATA, AND COPYRIGHTS Technical Data and Copyrights 927.408 Cosponsored research and development activities. Because of the Department of Energy's statutory duties to...

A Study of Emotions in Requirements Engineering

NASA Astrophysics Data System (ADS)

Colomo-Palacios, Ricardo; Hernández-López, Adrián; García-Crespo, Ángel; Soto-Acosta, Pedro

Requirements engineering (RE) is a crucial activity in software development projects. This phase in the software development cycle is knowledge intensive, and thus, human capital intensive. From the human point of view, emotions play an important role in behavior and can even act as behavioral motivators. Thus, if we consider that RE represents a set of knowledge-intensive tasks, which include acceptance and negotiation activities, then the emotional factor represents a key element in these issues. However, the emotional factor in RE has not received the attention it deserves. This paper aims to integrate the stakeholder's emotions into the requirement process, proposing to catalogue them like any other factor in the process such as clarity or stability. Results show that high arousal and low pleasure levels are predictors of high versioning requirements.

FGF-Dependent, Context-Driven Role for FRS Adapters in the Early Telencephalon

PubMed Central

Gutin, Grigoriy; Blackwood, Christopher A.; Kamatkar, Nachiket G.; Lee, Kyung W.; Fishell, Gordon; Wang, Fen

2017-01-01

FGF signaling, an important component of intercellular communication, is required in many tissues throughout development to promote diverse cellular processes. Whether FGF receptors (FGFRs) accomplish such varied tasks in part by activating different intracellular transducers in different contexts remains unclear. Here, we used the developing mouse telencephalon as an example to study the role of the FRS adapters FRS2 and FRS3 in mediating the functions of FGFRs. Using tissue-specific and germline mutants, we examined the requirement of Frs genes in two FGFR-dependent processes. We found that Frs2 and Frs3 are together required for the differentiation of a subset of medial ganglionic eminence (MGE)-derived neurons, but are dispensable for the survival of early telencephalic precursor cells, in which any one of three FGFRs (FGFR1, FGFR2, or FGFR3) is sufficient for survival. Although FRS adapters are dispensable for ERK-1/2 activation, they are required for AKT activation within the subventricular zone of the developing MGE. Using an FRS2,3-binding site mutant of Fgfr1, we established that FRS adapters are necessary for mediating most or all FGFR1 signaling, not only in MGE differentiation, but also in cell survival, implying that other adapters mediate at least in part the signaling from FGFR2 and FGFR3. Our study provides an example of a contextual role for an intracellular transducer and contributes to our understanding of how FGF signaling plays diverse developmental roles. SIGNIFICANCE STATEMENT FGFs promote a range of developmental processes in many developing tissues and at multiple developmental stages. The mechanisms underlying this multifunctionality remain poorly defined in vivo. Using telencephalon development as an example, we show here that FRS adapters exhibit some selectivity in their requirement for mediating FGF receptor (FGFR) signaling and activating downstream mediators that depend on the developmental process, with a requirement in neuronal differentiation but not cell survival. Differential engagement of FRS and non-FRS intracellular adapters downstream of FGFRs could therefore in principle explain how FGFs play several distinct roles in other developing tissues and developmental stages. PMID:28483978

FGF-Dependent, Context-Driven Role for FRS Adapters in the Early Telencephalon.

PubMed

Nandi, Sayan; Gutin, Grigoriy; Blackwood, Christopher A; Kamatkar, Nachiket G; Lee, Kyung W; Fishell, Gordon; Wang, Fen; Goldfarb, Mitchell; Hébert, Jean M

2017-06-07

FGF signaling, an important component of intercellular communication, is required in many tissues throughout development to promote diverse cellular processes. Whether FGF receptors (FGFRs) accomplish such varied tasks in part by activating different intracellular transducers in different contexts remains unclear. Here, we used the developing mouse telencephalon as an example to study the role of the FRS adapters FRS2 and FRS3 in mediating the functions of FGFRs. Using tissue-specific and germline mutants, we examined the requirement of Frs genes in two FGFR-dependent processes. We found that Frs2 and Frs3 are together required for the differentiation of a subset of medial ganglionic eminence (MGE)-derived neurons, but are dispensable for the survival of early telencephalic precursor cells, in which any one of three FGFRs (FGFR1, FGFR2, or FGFR3) is sufficient for survival. Although FRS adapters are dispensable for ERK-1/2 activation, they are required for AKT activation within the subventricular zone of the developing MGE. Using an FRS2,3-binding site mutant of Fgfr1 , we established that FRS adapters are necessary for mediating most or all FGFR1 signaling, not only in MGE differentiation, but also in cell survival, implying that other adapters mediate at least in part the signaling from FGFR2 and FGFR3. Our study provides an example of a contextual role for an intracellular transducer and contributes to our understanding of how FGF signaling plays diverse developmental roles. SIGNIFICANCE STATEMENT FGFs promote a range of developmental processes in many developing tissues and at multiple developmental stages. The mechanisms underlying this multifunctionality remain poorly defined in vivo Using telencephalon development as an example, we show here that FRS adapters exhibit some selectivity in their requirement for mediating FGF receptor (FGFR) signaling and activating downstream mediators that depend on the developmental process, with a requirement in neuronal differentiation but not cell survival. Differential engagement of FRS and non-FRS intracellular adapters downstream of FGFRs could therefore in principle explain how FGFs play several distinct roles in other developing tissues and developmental stages. Copyright © 2017 the authors 0270-6474/17/375690-09$15.00/0.

Moments of movement: active learning and practice development.

PubMed

Dewing, Jan

2010-01-01

As our understanding of practice development becomes more sophisticated, we enhance our understanding of how the facilitation of learning in and from practice, can be more effectively achieved. This paper outlines an approach for enabling and maximizing learning within practice development known as 'Active Learning'. It considers how, given establishing a learning culture is a prerequisite for the sustainability of PD within organisations, practice developers can do more to maximize learning for practitioners and other stakeholders. Active Learning requires that more attention be given by organisations committed to PD, at a corporate and strategic level for how learning strategies are developed in the workplace. Specifically, a move away from a heavy reliance on training may be required. Practice development facilitators also need to review: how they organise and offer learning, so that learning strategies are consistent with the vision, aims and processes of PD; have skills in the planning, delivery and evaluation of learning as part of their role and influence others who provide more traditional methods of training and education.

The Effect of Outdoor Learning Activities on the Development of Preschool Children

ERIC Educational Resources Information Center

Yildirim, Günseli; Özyilmaz Akamca, Güzin

2017-01-01

Learning ought to be supported by both in class activities and outdoor activities contributing to structuring knowledge. Outdoor activities allow children to actively participate and to learn by doing. Learning requires a lot of work and activities. These activities, which provide primary experiences, help children to change theoretical knowledge…

International perspectives on the physical inactivity crisis--structural solutions over evidence generation?

PubMed

Bauman, Adrian; Finegood, Diane T; Matsudo, Victor

2009-10-01

Many programs to increase physical activity have been evaluated in developed countries, where 'leisure time physical activity' is the most frequent domain for interventions. In developing countries, and also with reference to global obesity prevention, different kinds of interventions targeting 'total physical activity' are needed. This requires efforts across agencies and sectors, and in the domains of work, active transport, reduced sitting time, as well as leisure time physical activity promotion. In considering possible solutions, this commentary examined the use of complex systems, where integrated efforts across sectors and agencies might, in combination, contribute to increasing total physical activity. The key sets of actions required globally to increase physical activity were, in our opinion, [i] efforts to disseminate individual-level behavior change programs to reach much larger populations rather than volunteers, [ii] social marketing and mass communication campaigns to change social norms in the community and among professionals and policymakers, [iii] efforts to influence the social and physical environment to make them more conducive to physical activity, and [iv] the development and implementation of national physical activity plans and strategies, with sufficient timelines and resources to achieve measurable change.

An Extensible, User- Modifiable Framework for Planning Activities

NASA Technical Reports Server (NTRS)

Joshing, Joseph C.; Abramyan, Lucy; Mickelson, Megan C.; Wallick, Michael N.; Kurien, James A.; Crockett, Thomasa M.; Powell, Mark W.; Pyrzak, Guy; Aghevli, Arash

2013-01-01

This software provides a development framework that allows planning activities for the Mars Science Laboratory rover to be altered at any time, based on changes of the Activity Dictionary. The Activity Dictionary contains the definition of all activities that can be carried out by a particular asset (robotic or human). These definitions (and combinations of these definitions) are used by mission planners to give a daily plan of what a mission should do. During the development and course of the mission, the Activity Dictionary and actions that are going to be carried out will often be changed. Previously, such changes would require a change to the software and redeployment. Now, the Activity Dictionary authors are able to customize activity definitions, parameters, and resource usage without requiring redeployment. This software provides developers and end users the ability to modify the behavior of automatically generated activities using a script. This allows changes to the software behavior without incurring the burden of redeployment. This software is currently being used for the Mars Science Laboratory, and is in the process of being integrated into the LADEE (Lunar Atmosphere and Dust Environment Explorer) mission, as well as the International Space Station.

The CSR-1 endogenous RNAi pathway ensures accurate transcriptional reprogramming during the oocyte-to-embryo transition in Caenorhabditis elegans.

PubMed

Fassnacht, Christina; Tocchini, Cristina; Kumari, Pooja; Gaidatzis, Dimos; Stadler, Michael B; Ciosk, Rafal

2018-03-01

Endogenous RNAi (endoRNAi) is a conserved mechanism for fine-tuning gene expression. In the nematode Caenorhabditis elegans, several endoRNAi pathways are required for the successful development of reproductive cells. The CSR-1 endoRNAi pathway promotes germ cell development, primarily by facilitating the expression of germline genes. In this study, we report a novel function for the CSR-1 pathway in preventing premature activation of embryonic transcription in the developing oocytes, which is accompanied by a general Pol II activation. This CSR-1 function requires its RNase activity, suggesting that, by controlling the levels of maternal mRNAs, CSR-1-dependent endoRNAi contributes to an orderly reprogramming of transcription during the oocyte-to-embryo transition.

The CSR-1 endogenous RNAi pathway ensures accurate transcriptional reprogramming during the oocyte-to-embryo transition in Caenorhabditis elegans

PubMed Central

Tocchini, Cristina; Kumari, Pooja; Gaidatzis, Dimos

2018-01-01

Endogenous RNAi (endoRNAi) is a conserved mechanism for fine-tuning gene expression. In the nematode Caenorhabditis elegans, several endoRNAi pathways are required for the successful development of reproductive cells. The CSR-1 endoRNAi pathway promotes germ cell development, primarily by facilitating the expression of germline genes. In this study, we report a novel function for the CSR-1 pathway in preventing premature activation of embryonic transcription in the developing oocytes, which is accompanied by a general Pol II activation. This CSR-1 function requires its RNase activity, suggesting that, by controlling the levels of maternal mRNAs, CSR-1-dependent endoRNAi contributes to an orderly reprogramming of transcription during the oocyte-to-embryo transition. PMID:29579041

Mechanical activation of mammalian target of rapamycin pathway is required for cartilage development

PubMed Central

Guan, Yingjie; Yang, Xu; Yang, Wentian; Charbonneau, Cherie; Chen, Qian

2014-01-01

Mechanical stress regulates development by modulating cell signaling and gene expression. However, the cytoplasmic components mediating mechanotransduction remain unclear. In this study, elimination of muscle contraction during chicken embryonic development resulted in a reduction in the activity of mammalian target of rapamycin (mTOR) in the cartilaginous growth plate. Inhibition of mTOR activity led to significant inhibition of chondrocyte proliferation, cartilage tissue growth, and expression of chondrogenic genes, including Indian hedgehog (Ihh), a critical mediator of mechanotransduction. Conversely, cyclic loading (1 Hz, 5% matrix deformation) of embryonic chicken growth plate chondrocytes in 3-dimensional (3D) collagen scaffolding induced sustained activation of mTOR. Mechanical activation of mTOR occurred in serum-free medium, indicating that it is independent of growth factor or nutrients. Treatment of chondrocytes with Rapa abolished mechanical activation of cell proliferation and Ihh gene expression. Cyclic loading of chondroprogenitor cells deficient in SH2-containing protein tyrosine phosphatase 2 (Shp2) further enhanced mechanical activation of mTOR, cell proliferation, and chondrogenic gene expression. This result suggests that Shp2 is an antagonist of mechanotransduction through inhibition of mTOR activity. Our data demonstrate that mechanical activation of mTOR is necessary for cell proliferation, chondrogenesis, and cartilage growth during bone development, and that mTOR is an essential mechanotransduction component modulated by Shp2 in the cytoplasm.—Guan, Y., Yang, X., Yang, W., Charbonneau, C., Chen, Q. Mechanical activation of mammalian target of rapamycin pathway is required for cartilage development. PMID:25002119

Transcriptome Profiling Identifies Multiplexin as a Target of SAGA Deubiquitinase Activity in Glia Required for Precise Axon Guidance During Drosophila Visual Development.

PubMed

Ma, Jingqun; Brennan, Kaelan J; D'Aloia, Mitch R; Pascuzzi, Pete E; Weake, Vikki M

2016-08-09

The Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex is a transcriptional coactivator with histone acetylase and deubiquitinase activities that plays an important role in visual development and function. In Drosophila melanogaster, four SAGA subunits are required for the deubiquitination of monoubiquitinated histone H2B (ubH2B): Nonstop, Sgf11, E(y)2, and Ataxin 7. Mutations that disrupt SAGA deubiquitinase activity cause defects in neuronal connectivity in the developing Drosophila visual system. In addition, mutations in SAGA result in the human progressive visual disorder spinocerebellar ataxia type 7 (SCA7). Glial cells play a crucial role in both the neuronal connectivity defect in nonstop and sgf11 flies, and in the retinal degeneration observed in SCA7 patients. Thus, we sought to identify the gene targets of SAGA deubiquitinase activity in glia in the Drosophila larval central nervous system. To do this, we enriched glia from wild-type, nonstop, and sgf11 larval optic lobes using affinity-purification of KASH-GFP tagged nuclei, and then examined each transcriptome using RNA-seq. Our analysis showed that SAGA deubiquitinase activity is required for proper expression of 16% of actively transcribed genes in glia, especially genes involved in proteasome function, protein folding and axon guidance. We further show that the SAGA deubiquitinase-activated gene Multiplexin (Mp) is required in glia for proper photoreceptor axon targeting. Mutations in the human ortholog of Mp, COL18A1, have been identified in a family with a SCA7-like progressive visual disorder, suggesting that defects in the expression of this gene in SCA7 patients could play a role in the retinal degeneration that is unique to this ataxia. Copyright © 2016 Ma et al.

Wnt signalling controls the response to mechanical loading during zebrafish joint development

PubMed Central

Brunt, Lucy H.; Begg, Katie; Kague, Erika; Cross, Stephen

2017-01-01

Joint morphogenesis requires mechanical activity during development. Loss of mechanical strain causes abnormal joint development, which can impact long-term joint health. Although cell orientation and proliferation are known to shape the joint, dynamic imaging of developing joints in vivo has not been possible in other species. Using genetic labelling techniques in zebrafish we were able, for the first time, to dynamically track cell behaviours in intact moving joints. We identify that proliferation and migration, which contribute to joint morphogenesis, are mechanically controlled and are significantly reduced in immobilised larvae. By comparison with strain maps of the developing skeleton, we identify canonical Wnt signalling as a candidate for transducing mechanical forces into joint cell behaviours. We show that, in the jaw, Wnt signalling is reduced specifically in regions of high strain in response to loss of muscle activity. By pharmacological manipulation of canonical Wnt signalling, we demonstrate that Wnt acts downstream of mechanical activity and is required for joint patterning and chondrocyte maturation. Wnt16, which is also downstream of muscle activity, controls proliferation and migration, but plays no role in chondrocyte intercalation. PMID:28684625

Electro-optical rendezvous and docking sensors

NASA Technical Reports Server (NTRS)

Tubbs, David J.; Kesler, Lynn O.; Sirko, Robert J.

1991-01-01

Electro-optical sensors provide unique and critical functionality for space missions requiring rendezvous, docking, and berthing. McDonnell Douglas is developing a complete rendezvous and docking system for both manned and unmanned missions. This paper examines our sensor development and the systems and missions which benefit from rendezvous and docking sensors. Simulation results quantifying system performance improvements in key areas are given, with associated sensor performance requirements. A brief review of NASA-funded development activities and the current performance of electro-optical sensors for space applications is given. We will also describe current activities at McDonnell Douglas for a fully functional demonstration to address specific NASA mission needs.

A Summary on Progress in Materials Development for Advanced Lithium-ion Cells for NASA's Exploration Missions

NASA Technical Reports Server (NTRS)

Reid, Concha M.

2011-01-01

Vehicles and stand-alone power systems that enable the next generation of human missions to the moon will require energy storage systems that are safer, lighter, and more compact than current state-of-the-art (SOA) aerospace quality lithium-ion (Li-ion) batteries. NASA is developing advanced Li-ion cells to enable or enhance future human missions to Near Earth Objects, such as asteroids, planets, moons, libration points, and orbiting structures. Advanced, high-performing materials are required to provide component-level performance that can offer the required gains at the integrated cell level. Although there is still a significant amount of work yet to be done, the present state of development activities has resulted in the synthesis of promising materials that approach the ultimate performance goals. This paper on interim progress of the development efforts will present performance of materials and cell components and will elaborate on the challenges of the development activities and proposed strategies to overcome technical issues.

Human-Centered Development of an Online Social Network for Metabolic Syndrome Management.

PubMed

Núñez-Nava, Jefersson; Orozco-Sánchez, Paola A; López, Diego M; Ceron, Jesus D; Alvarez-Rosero, Rosa E

2016-01-01

According to the International Diabetes Federation (IDF), a quarter of the world's population has Metabolic Syndrome (MS). To develop (and assess the users' degree of satisfaction of) an online social network for patients who suffer from Metabolic Syndrome, based on the recommendations and requirements of the Human-Centered Design. Following the recommendations of the ISO 9241-210 for Human-Centered Design (HCD), an online social network was designed to promote physical activity and healthy nutrition. In order to guarantee the active participation of the users during the development of the social network, a survey, an in-depth interview, a focal group, and usability tests were carried out with people suffering from MS. The study demonstrated how the different activities, recommendations, and requirements of the ISO 9241-210 are integrated into a traditional software development process. Early usability tests demonstrated that the user's acceptance and the effectiveness and efficiency of the social network are satisfactory.

Skilled movements require non-apoptotic Bax/Bak pathway-mediated corticospinal circuit reorganization

PubMed Central

Gu, Zirong; Serradj, Najet; Ueno, Masaki; Liang, Mishi; Li, Jie; Baccei, Mark L.; Martin, John H.; Yoshida, Yutaka

2017-01-01

Early postnatal mammals, including human babies, can perform only basic motor tasks. The acquisition of skilled behaviors occurs later, requiring anatomical changes in neural circuitry to support the development of coordinated activation or suppression of functionally related muscle groups. How this circuit reorganization occurs during postnatal development remains poorly understood. Here we explore the connectivity between corticospinal (CS) neurons in the motor cortex and muscles in mice. Using trans-synaptic viral and electrophysiological assays, we identify the early postnatal reorganization of CS circuitry for antagonistic muscle pairs. We further show that this synaptic rearrangement requires the activity-dependent, non-apoptotic Bax/Bak-caspase signaling cascade. Adult Bax/Bak mutant mice exhibit aberrant co-activation of antagonistic muscle pairs and skilled grasping deficits but normal reaching and retrieval behaviors. Our findings reveal key cellular and molecular mechanisms driving postnatal motor circuit reorganization and the resulting impacts on muscle activation patterns and the execution of skilled movements. PMID:28472660

The challenge of developing structural materials for fusion power systems

NASA Astrophysics Data System (ADS)

Bloom, Everett E.

1998-10-01

Nuclear fusion can be one of the most attractive sources of energy from the viewpoint of safety and minimal environmental impact. Central in the goal of designing a safe, environmentally benign, and economically competitive fusion power system is the requirement for high performance, low activation materials. The general performance requirements for such materials have been defined and it is clear that materials developed for other applications (e.g. aerospace, nuclear fission, fossil energy systems) will not fully meet the needs of fusion. Advanced materials, with composition and microstructure tailored to yield properties that will satisfy the specific requirements of fusion must be developed. The international fusion programs have made significant progress towards this goal. Compositional requirements for low activation lead to a focus of development efforts on silicon carbide composites, vanadium alloys, and advanced martensitic steels as candidate structural material systems. Control of impurities will be critically important in actually achieving low activation but this appears possible. Neutron irradiation produces significant changes in the mechanical and physical properties of each of these material systems raising feasibility questions and design limitations. A focus of the research and development effort is to understand these effects, and through the development of specific compositions and microstructures, produce materials with improved and adequate performance. Other areas of research that are synergistic with the development of radiation resistant materials include fabrication, joining technology, chemical compatibility with coolants and tritium breeders and specific questions relating to the unique characteristics of a given material (e.g. coatings to reduce gas permeation in SiC composites) or design concept (e.g. electrical insulator coatings for liquid metal concepts).

The (Possibly Negative) Effects of Physical Activity on Executive Functions: Implications of the Changing Metabolic Costs of Brain Development.

PubMed

Howard, Steven J; Cook, Caylee J; Said-Mohamed, Rihlat; Norris, Shane A; Draper, Catherine E

2016-09-01

An area of growth in physical activity research has involved investigating effects of physical activity on children's executive functions. Many of these efforts seek to increase the energy expenditure of young children as a healthy and low-cost way to affect physical, health, and cognitive outcomes. We review theory and research from neuroscience and evolutionary biology, which suggest that interventions seeking to increase the energy expenditure of young children must also consider the energetic trade-offs that occur to accommodate changing metabolic costs of brain development. According to Life History Theory, and supported by recent evidence, the high relative energy-cost of early brain development requires that other energy-demanding functions of development (ie, physical growth, activity) be curtailed. This is important for interventions seeking to dramatically increase the energy expenditure of young children who have little excess energy available, with potentially negative cognitive consequences. Less energy-demanding physical activities, in contrast, may yield psychosocial and cognitive benefits while not overburdening an underweight child's already scarce energy supply. While further research is required to establish the extent to which increases in energy-demanding physical activities may compromise or displace energy available for brain development, we argue that action cannot await these findings.

LDRD 2014 Annual Report: Laboratory Directed Research and Development Program Activities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hatton, Diane

Each year, Brookhaven National Laboratory (BNL) is required to provide a program description and overview of its Laboratory Directed Research and Development Program (LDRD) to the Department of Energy (DOE) in accordance with DOE Order 413.2B dated April 19, 2006. This report provides a detailed look at the scientific and technical activities for each of the LDRD projects funded by BNL in FY 2014, as required. In FY 2014, the BNL LDRD Program funded 40 projects, 8 of which were new starts, at a total cost of $9.6M.

LDRD 2012 Annual Report: Laboratory Directed Research and Development Program Activities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bookless, William

Each year, Brookhaven National Laboratory (BNL) is required to provide a program description and overview of its Laboratory Directed Research and Development Program (LDRD) to the Department of Energy in accordance with DOE Order 413.2B dated April 19, 2006. This report provides a detailed look at the scientific and technical activities for each of the LDRD projects funded by BNL in FY2012, as required. In FY2012, the BNL LDRD Program funded 52 projects, 14 of which were new starts, at a total cost of $10,061,292.

LDRD 2015 Annual Report: Laboratory Directed Research and Development Program Activities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hatton, D.

Each year, Brookhaven National Laboratory (BNL) is required to provide a program description and overview of its Laboratory Directed Research and Development Program (LDRD) to the Department of Energy (DOE) in accordance with DOE Order 413.2B dated April 19, 2006. This report provides a detailed look at the scientific and technical activities for each of the LDRD projects funded by BNL in FY 2015, as required. In FY 2015, the BNL LDRD Program funded 43 projects, 12 of which were new starts, at a total cost of $9.5M.

Neuron class-specific requirements for Fragile X Mental Retardation Protein in critical period development of calcium signaling in learning and memory circuitry.

PubMed

Doll, Caleb A; Broadie, Kendal

2016-05-01

Neural circuit optimization occurs through sensory activity-dependent mechanisms that refine synaptic connectivity and information processing during early-use developmental critical periods. Fragile X Mental Retardation Protein (FMRP), the gene product lost in Fragile X syndrome (FXS), acts as an activity sensor during critical period development, both as an RNA-binding translation regulator and channel-binding excitability regulator. Here, we employ a Drosophila FXS disease model to assay calcium signaling dynamics with a targeted transgenic GCaMP reporter during critical period development of the mushroom body (MB) learning/memory circuit. We find FMRP regulates depolarization-induced calcium signaling in a neuron-specific manner within this circuit, suppressing activity-dependent calcium transients in excitatory cholinergic MB input projection neurons and enhancing calcium signals in inhibitory GABAergic MB output neurons. Both changes are restricted to the developmental critical period and rectified at maturity. Importantly, conditional genetic (dfmr1) rescue of null mutants during the critical period corrects calcium signaling defects in both neuron classes, indicating a temporally restricted FMRP requirement. Likewise, conditional dfmr1 knockdown (RNAi) during the critical period replicates constitutive null mutant defects in both neuron classes, confirming cell-autonomous requirements for FMRP in developmental regulation of calcium signaling dynamics. Optogenetic stimulation during the critical period enhances depolarization-induced calcium signaling in both neuron classes, but this developmental change is eliminated in dfmr1 null mutants, indicating the activity-dependent regulation requires FMRP. These results show FMRP shapes neuron class-specific calcium signaling in excitatory vs. inhibitory neurons in developing learning/memory circuitry, and that FMRP mediates activity-dependent regulation of calcium signaling specifically during the early-use critical period. Copyright © 2016 Elsevier Inc. All rights reserved.

A Framework for Assessing Continuing Professional Development Activities for Satisfying Pharmacy Revalidation Requirements

ERIC Educational Resources Information Center

Donyai, Parastou; Alexander, Angela M.; Denicolo, Pam M.

2013-01-01

Introduction: The United Kingdom's pharmacy regulator contemplated using continuing professional development (CPD) in pharmacy revalidation in 2009, simultaneously asking pharmacy professionals to demonstrate the value of their CPD by showing its relevance and impact. The idea of linking new CPD requirements with revalidation was yet to be…

Exploring the Development of Competence in Lean Management through Action Learning Groups: A Study of the Introduction of Lean to a Facilities Management Function

ERIC Educational Resources Information Center

Wyton, Paul; Payne, Robin

2014-01-01

The introduction of a Lean approach to management requires the development of understanding and capability. This in turn requires a structured training intervention and other supporting activities. This paper explores, through a case study, the way in which action learning groups (ALGs) supported the development of Lean capabilities in the…

The Relationship between Physical Activity and Productivity

DTIC Science & Technology

1984-04-01

suggestions as our advisor. Captain Jeffrey S. Austin, USAF, of the Leadership and Management Development Center (LMDC) served unofficially yet willingly as...youthful and healthy population. It is recommended that the USAF: (1) develop fitness standards based on wartime requirements for skills requiring high...encouraged its military personnel to develop and maintain physical fitness. In the USAF’s view, regular physical conditioning and a balanced diet

Crew interface specifications preparation for in-flight maintenance and stowage functions

NASA Technical Reports Server (NTRS)

Parker, F. W.; Carlton, B. E.

1972-01-01

The findings and data products developed during the Phase 2 crew interface specification study are presented. Five new NASA general specifications were prepared: operations location coding system for crew interfaces; loose equipment and stowage management requirements; loose equipment and stowage data base information requirements; spacecraft loose equipment stowage drawing requirements; and inflight stowage management data requirements. Additional data was developed defining inflight maintenance processes and related data concepts for inflight troubleshooting, remove/repair/replace and scheduled maintenance activities. The process of maintenance task and equipment definition during spacecraft design and development was also defined and related data concepts were identified for futher development into formal NASA specifications during future follow-on study phases of the contract.

A novel positive transcriptional feedback loop in midbrain-hindbrain boundary development is revealed through analysis of the zebrafish pax2.1 promoter in transgenic lines.

PubMed

Picker, Alexander; Scholpp, Steffen; Böhli, Heike; Takeda, Hiroyuki; Brand, Michael

2002-07-01

The pax2.1 gene encodes a paired-box transcription factor that is one of the earliest genes to be specifically activated in development of the midbrain and midbrain-hindbrain boundary (MHB), and is required for the development and organizer activity of this territory. To understand how this spatially restricted transcriptional activity of pax2.1 is achieved, we have isolated and characterized the pax2.1-promoter using a lacZ and a GFP reporter gene in transient injection assays and transgenic lines. Stable transgenic expression of this reporter gene shows that a 5.3-kb fragment of the 5' region contains most, but not all, elements required for driving pax2.1 expression. The expressing tissues include the MHB, hindbrain, spinal cord, ear and pronephros. Transgene activation in the pronephros and developing ear suggests that these pax2.1-expressing tissues are composed of independently regulated subdomains. In addition, ectopic but spatially restricted activation of the reporter genes in rhombomeres 3 and 5 and in the forebrain, which do not normally express endogenous pax2.1, demonstrates the importance of negative regulation of pax2.1. Comparison of transgene expression in wild-type and homozygous pax2.1 mutant no isthmus (noi) embryos reveals that the transgene contains control element(s) for a novel, positive transcriptional feedback loop in MHB development. Transcription of endogenous pax2.1 at the MHB is known to be initially Pax2.1 independent, during activation in late gastrulation. In contrast, transgene expression requires the endogenous Pax2.1 function. Transplantations, mRNA injections and morpholino knock-down experiments show that this feedback regulation of pax2.1 transcription occurs cell-autonomously, and that it requires eng2 and eng3 as known targets for Pax2.1 regulation. We suggest that this novel feedback loop may allow continuation of pax2.1 expression, and hence development of the MHB organizer, to become independent of the patterning machinery of the gastrula embryo.

Integrating Safety and Mission Assurance into Systems Engineering Modeling Practices

NASA Technical Reports Server (NTRS)

Beckman, Sean; Darpel, Scott

2015-01-01

During the early development of products, flight, or experimental hardware, emphasis is often given to the identification of technical requirements, utilizing such tools as use case and activity diagrams. Designers and project teams focus on understanding physical and performance demands and challenges. It is typically only later, during the evaluation of preliminary designs that a first pass, if performed, is made to determine the process, safety, and mission quality assurance requirements. Evaluation early in the life cycle, though, can yield requirements that force a fundamental change in design. This paper discusses an alternate paradigm for using the concepts of use case or activity diagrams to identify safety hazard and mission quality assurance risks and concerns using the same systems engineering modeling tools being used to identify technical requirements. It contains two examples of how this process might be used in the development of a space flight experiment, and the design of a Human Powered Pizza Delivery Vehicle, along with the potential benefits to decrease development time, and provide stronger budget estimates.

Joint Optics Structures Experiment (JOSE)

NASA Technical Reports Server (NTRS)

Founds, David

1987-01-01

The objectives of the JOSE program is to develop, demonstrate, and evaluate active vibration suppression techniques for Directed Energy Weapons (DEW). DEW system performance is highly influenced by the line-of-sight (LOS) stability and in some cases by the wave front quality. The missions envisioned for DEW systems by the Strategic Defense Initiative require LOS stability and wave front quality to be significantly improved over any current demonstrated capability. The Active Control of Space Structures (ACOSS) program led to the development of a number of promising structural control techniques. DEW structures are vastly more complex than any structures controlled to date. They will be subject to disturbances with significantly higher magnitudes and wider bandwidths, while holding higher tolerances on allowable motions and deformations. Meeting the performance requirements of the JOSE program requires upgrading the ACOSS techniques to meet new more stringent requirements, the development of requisite sensors and acturators, improved control processors, highly accurate system identification methods, and the integration of hardware and methodologies into a successful demonstration.

Requirement Specifications for a Design and Verification Unit.

ERIC Educational Resources Information Center

Pelton, Warren G.; And Others

A research and development activity to introduce new and improved education and training technology into Bureau of Medicine and Surgery training is recommended. The activity, called a design and verification unit, would be administered by the Education and Training Sciences Department. Initial research and development are centered on the…

10 CFR 70.23 - Requirements for the approval of applications.

Code of Federal Regulations, 2010 CFR

2010-01-01

... be used for the conduct of research or development activities of a type specified in section 31 of... types of research and development activities specified in section 31 are those relating to: (1) Nuclear processes; (2) The theory and production of atomic energy, including processes, materials, and devices...

Active Control of Cryogenic Propellants in Space

NASA Technical Reports Server (NTRS)

Notardonato, William

2011-01-01

A new era of space exploration is being planned. Exploration architectures under consideration require the long term storage of cryogenic propellants in space. This requires development of active control systems to mitigate the effect of heat leak. This work summarizes current state of the art, proposes operational design strategies and presents options for future architectures. Scaling and integration of active systems will be estimated. Ideal long range spacecraft systems will be proposed with Exploration architecture benefits considered.

24 CFR 1000.40 - Do lead-based paint poisoning prevention requirements apply to affordable housing activities...

Code of Federal Regulations, 2013 CFR

2013-04-01

... AMERICAN HOUSING ACTIVITIES General § 1000.40 Do lead-based paint poisoning prevention requirements apply..., subparts A, B, H, J, K, M and R of this title, which implement the Lead-Based Paint Poisoning Prevention... 24 Housing and Urban Development 4 2013-04-01 2013-04-01 false Do lead-based paint poisoning...

24 CFR 1000.40 - Do lead-based paint poisoning prevention requirements apply to affordable housing activities...

Code of Federal Regulations, 2014 CFR

2014-04-01

... AMERICAN HOUSING ACTIVITIES General § 1000.40 Do lead-based paint poisoning prevention requirements apply..., subparts A, B, H, J, K, M and R of this title, which implement the Lead-Based Paint Poisoning Prevention... 24 Housing and Urban Development 4 2014-04-01 2014-04-01 false Do lead-based paint poisoning...

24 CFR 1000.40 - Do lead-based paint poisoning prevention requirements apply to affordable housing activities...

Code of Federal Regulations, 2010 CFR

2010-04-01

... AMERICAN HOUSING ACTIVITIES General § 1000.40 Do lead-based paint poisoning prevention requirements apply..., subparts A, B, H, J, K, M and R of this title, which implement the Lead-Based Paint Poisoning Prevention... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Do lead-based paint poisoning...

24 CFR 1000.40 - Do lead-based paint poisoning prevention requirements apply to affordable housing activities...

Code of Federal Regulations, 2011 CFR

2011-04-01

... AMERICAN HOUSING ACTIVITIES General § 1000.40 Do lead-based paint poisoning prevention requirements apply..., subparts A, B, H, J, K, M and R of this title, which implement the Lead-Based Paint Poisoning Prevention... 24 Housing and Urban Development 4 2011-04-01 2011-04-01 false Do lead-based paint poisoning...

24 CFR 1000.40 - Do lead-based paint poisoning prevention requirements apply to affordable housing activities...

Code of Federal Regulations, 2012 CFR

2012-04-01

... AMERICAN HOUSING ACTIVITIES General § 1000.40 Do lead-based paint poisoning prevention requirements apply..., subparts A, B, H, J, K, M and R of this title, which implement the Lead-Based Paint Poisoning Prevention... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Do lead-based paint poisoning...

Advanced Biotelemetry Systems for Space Life Sciences: PH Telemetry

NASA Technical Reports Server (NTRS)

Hines, John W.; Somps, Chris; Ricks, Robert; Kim, Lynn; Connolly, John P. (Technical Monitor)

1995-01-01

The SENSORS 2000! (S2K!) program at NASA's Ames Research Center is currently developing a biotelemetry system for monitoring pH and temperature in unrestrained subjects. This activity is part of a broader scope effort to provide an Advanced Biotelemetry System (ABTS) for use in future space life sciences research. Many anticipated research endeavors will require biomedical and biochemical sensors and related instrumentation to make continuous inflight measurements in a variable-gravity environment. Since crew time is limited, automated data acquisition, data processing, data storage, and subject health monitoring are required. An automated biochemical and physiological data acquisition system based on non invasive or implantable biotelemetry technology will meet these requirements. The ABTS will ultimately acquire a variety of physiological measurands including temperature, biopotentials (e.g. ECG, EEG, EMG, EOG), blood pressure, flow and dimensions, as well as chemical and biological parameters including pH. Development activities are planned in evolutionary, leveraged steps. Near-term activities include 1) development of a dual channel pH/temperature telemetry system, and 2) development of a low bandwidth, 4-channel telemetry system, that measures temperature, heart rate, pressure, and pH. This abstract describes the pH/temperature telemeter.

Directory of Energy Information Administration model abstracts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1987-08-11

This report contains brief statements from the model managers about each model's title, acronym, purpose, and status, followed by more detailed information on characteristics, uses, and requirements. Sources for additional information are identified. All models ''active'' through March 1987 are included. The main body of this directory is an alphabetical list of all active EIA models. Appendix A identifies major EIA modeling systems and the models within these systems, and Appendix B identifies active EIA models by type (basic, auxiliary, and developing). A basic model is one designated by the EIA Administrator as being sufficiently important to require sustained supportmore » and public scrutiny. An auxiliary model is one designated by the EIA Administrator as being used only occasionally in analyses, and therefore requires minimal levels of documentation. A developing model is one designated by the EIA Administrator as being under development and yet of sufficient interest to require a basic level of documentation at a future date. EIA also leases models developed by proprietary software vendors. Documentation for these ''proprietary'' models is the responsibility of the companies from which they are leased. EIA has recently leased models from Chase Econometrics, Inc., Data Resources, Inc. (DRI), the Oak Ridge National Laboratory (ORNL), and Wharton Econometric Forecasting Associates (WEFA). Leased models are not abstracted here. The directory is intended for the use of energy and energy-policy analysts in the public and private sectors.« less

Electric Propulsion Requirements and Mission Analysis Under NASA's In-Space Propulsion Technology Project

NASA Technical Reports Server (NTRS)

Dudzinski, Leonard a.; Pencil, Eric J.; Dankanich, John W.

2007-01-01

The In-Space Propulsion Technology Project (ISPT) is currently NASA's sole investment in electric propulsion technologies. This project is managed at NASA Glenn Research Center (GRC) for the NASA Headquarters Science Mission Directorate (SMD). The objective of the electric propulsion project area is to develop near-term and midterm electric propulsion technologies to enhance or enable future NASA science missions while minimizing risk and cost to the end user. Systems analysis activities sponsored by ISPT seek to identify future mission applications in order to quantify mission requirements, as well as develop analytical capability in order to facilitate greater understanding and application of electric propulsion and other propulsion technologies in the ISPT portfolio. These analyses guide technology investments by informing decisions and defining metrics for technology development to meet identified mission requirements. This paper discusses the missions currently being studied for electric propulsion by the ISPT project, and presents the results of recent electric propulsion (EP) mission trades. Recent ISPT systems analysis activities include: an initiative to standardize life qualification methods for various electric propulsion systems in order to retire perceived risk to proposed EP missions; mission analysis to identify EP requirements from Discovery, New Frontiers, and Flagship classes of missions; and an evaluation of system requirements for radioisotope-powered electric propulsion. Progress and early results of these activities is discussed where available.

Integration of safety engineering into a cost optimized development program.

NASA Technical Reports Server (NTRS)

Ball, L. W.

1972-01-01

A six-segment management model is presented, each segment of which represents a major area in a new product development program. The first segment of the model covers integration of specialist engineers into 'systems requirement definition' or the system engineering documentation process. The second covers preparation of five basic types of 'development program plans.' The third segment covers integration of system requirements, scheduling, and funding of specialist engineering activities into 'work breakdown structures,' 'cost accounts,' and 'work packages.' The fourth covers 'requirement communication' by line organizations. The fifth covers 'performance measurement' based on work package data. The sixth covers 'baseline requirements achievement tracking.'

24 CFR 582.325 - Outreach activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMMUNITY FACILITIES SHELTER PLUS CARE Program Requirements § 582.325 Outreach...

24 CFR 1000.512 - Are performance reports required?

Code of Federal Regulations, 2014 CFR

2014-04-01

... URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring, Oversight and Accountability... the following: (1) A comparison of actual accomplishments to the planned activities established for the period; (2) The reasons for slippage if established planned activities were not met; and (3...

24 CFR 1000.512 - Are performance reports required?

Code of Federal Regulations, 2013 CFR

2013-04-01

... URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring, Oversight and Accountability... the following: (1) A comparison of actual accomplishments to the planned activities established for the period; (2) The reasons for slippage if established planned activities were not met; and (3...

Developing a Crew Time Model for Human Exploration Missions to Mars

NASA Technical Reports Server (NTRS)

Battfeld, Bryan; Stromgren, Chel; Shyface, Hilary; Cirillo, William; Goodliff, Kandyce

2015-01-01

Candidate human missions to Mars require mission lengths that could extend beyond those that have previously been demonstrated during crewed Lunar (Apollo) and International Space Station (ISS) missions. The nature of the architectures required for deep space human exploration will likely necessitate major changes in how crews operate and maintain the spacecraft. The uncertainties associated with these shifts in mission constructs - including changes to habitation systems, transit durations, and system operations - raise concerns as to the ability of the crew to complete required overhead activities while still having time to conduct a set of robust exploration activities. This paper will present an initial assessment of crew operational requirements for human missions to the Mars surface. The presented results integrate assessments of crew habitation, system maintenance, and utilization to present a comprehensive analysis of potential crew time usage. Destination operations were assessed for a short (approx. 50 day) and long duration (approx. 500 day) surface habitation case. Crew time allocations are broken out by mission segment, and the availability of utilization opportunities was evaluated throughout the entire mission progression. To support this assessment, the integrated crew operations model (ICOM) was developed. ICOM was used to parse overhead, maintenance and system repair, and destination operations requirements within each mission segment - outbound transit, Mars surface duration, and return transit - to develop a comprehensive estimation of exploration crew time allocations. Overhead operational requirements included daily crew operations, health maintenance activities, and down time. Maintenance and repair operational allocations are derived using the Exploration Maintainability and Analysis Tool (EMAT) to develop a probabilistic estimation of crew repair time necessary to maintain systems functionality throughout the mission.

OSTA commonality analysis, volume 1

NASA Technical Reports Server (NTRS)

1981-01-01

The NASA End-to-End Data System (NEEDS) concept to achieve the required improvement in data systems capability where this activity may be subdivided into a logical set of development activities is reviewed.

Using assistive robots to promote inclusive education.

PubMed

Encarnação, P; Leite, T; Nunes, C; Nunes da Ponte, M; Adams, K; Cook, A; Caiado, A; Pereira, J; Piedade, G; Ribeiro, M

2017-05-01

This paper describes the development and test of physical and virtual integrated augmentative manipulation and communication assistive technologies (IAMCATs) that enable children with motor and speech impairments to manipulate educational items by controlling a robot with a gripper, while communicating through a speech generating device. Nine children with disabilities, nine regular and nine special education teachers participated in the study. Teachers adapted academic activities so they could also be performed by the children with disabilities using the IAMCAT. An inductive content analysis of the teachers' interviews before and after the intervention was performed. Teachers considered the IAMCAT to be a useful resource that can be integrated into the regular class dynamics respecting their curricular planning. It had a positive impact on children with disabilities and on the educational community. However, teachers pointed out the difficulties in managing the class, even with another adult present, due to the extra time required by children with disabilities to complete the activities. The developed assistive technologies enable children with disabilities to participate in academic activities but full inclusion would require another adult in class and strategies to deal with the additional time required by children to complete the activities. Implications for Rehabilitation Integrated augmentative manipulation and communication assistive technologies are useful resources to promote the participation of children with motor and speech impairments in classroom activities. Virtual tools, running on a computer screen, may be easier to use but further research is needed in order to evaluate its effectiveness when compared to physical tools. Full participation of children with motor and speech impairments in academic activities using these technologies requires another adult in class and adequate strategies to manage the extra time the child with disabilities may require to complete the activities.

The kinematic architecture of the Active Headframe: A new head support for awake brain surgery.

PubMed

Malosio, Matteo; Negri, Simone Pio; Pedrocchi, Nicola; Vicentini, Federico; Cardinale, Francesco; Tosatti, Lorenzo Molinari

2012-01-01

This paper presents the novel hybrid kinematic structure of the Active Headframe, a robotic head support to be employed in brain surgery operations for an active and dynamic control of the patient's head position and orientation, particularly addressing awake surgery requirements. The topology has been conceived in order to satisfy all the installation, functional and dynamic requirements. A kinetostatic optimization has been performed to obtain the actual geometric dimensions of the prototype currently being developed.

75 FR 63514 - Agency Information Collection Activities: Submission to OMB for Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-15

... be required to develop and submit a retained earnings accumulation plan. The rule generally requires... hours = 1,000 hours. Retained earnings accumulation Plans: 3 corporates x 50 hours = 150 hours. Notice... imposes new requirements concerning the use of nationally recognized statistical rating agencies, which...

20 CFR 666.110 - May a Governor require additional indicators of performance?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false May a Governor require additional indicators... of Performance § 666.110 May a Governor require additional indicators of performance? Yes, Governors may develop additional indicators of performance for adults, youth and dislocated worker activities...

20 CFR 666.110 - May a Governor require additional indicators of performance?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false May a Governor require additional indicators... of Performance § 666.110 May a Governor require additional indicators of performance? Yes, Governors may develop additional indicators of performance for adults, youth and dislocated worker activities...

Emerging Definition of Next-Generation of Aeronautical Communications

NASA Technical Reports Server (NTRS)

Kerczewski, Robert J.

2006-01-01

Aviation continues to experience rapid growth. In regions such as the United States and Europe air traffic congestion is constraining operations, leading to major new efforts to develop methodologies and infrastructures to enable continued aviation growth through transformational air traffic management systems. Such a transformation requires better communications linking airborne and ground-based elements. Technologies for next-generation communications, the required capacities, frequency spectrum of operation, network interconnectivity, and global interoperability are now receiving increased attention. A number of major planning and development efforts have taken place or are in process now to define the transformed airspace of the future. These activities include government and industry led efforts in the United States and Europe, and by international organizations. This paper will review the features, approaches, and activities of several representative planning and development efforts, and identify the emerging global consensus on requirements of next generation aeronautical communications systems for air traffic control.

Analysis, Modeling, and Simulation (AMS) Testbed Development and Evaluation to Support Dynamic Mobility Applications (DMA) and Active Transportation and Demand Management (ATDM) Programs - AMS Testbed Detailed Requirements

DOT National Transportation Integrated Search

2016-04-20

The primary objective of this project is to develop multiple simulation Testbeds/transportation models to evaluate the impacts of DMA connected vehicle applications and the active and dynamic transportation management (ATDM) strategies. The outputs (...

Emerging feed-forward inhibition allows the robust formation of direction selectivity in the developing ferret visual cortex

PubMed Central

Escobar, Gina M.; Maffei, Arianna; Miller, Paul

2014-01-01

The computation of direction selectivity requires that a cell respond to joint spatial and temporal characteristics of the stimulus that cannot be separated into independent components. Direction selectivity in ferret visual cortex is not present at the time of eye opening but instead develops in the days and weeks following eye opening in a process that requires visual experience with moving stimuli. Classic Hebbian or spike timing-dependent modification of excitatory feed-forward synaptic inputs is unable to produce direction-selective cells from unselective or weakly directionally biased initial conditions because inputs eventually grow so strong that they can independently drive cortical neurons, violating the joint spatial-temporal activation requirement. Furthermore, without some form of synaptic competition, cells cannot develop direction selectivity in response to training with bidirectional stimulation, as cells in ferret visual cortex do. We show that imposing a maximum lateral geniculate nucleus (LGN)-to-cortex synaptic weight allows neurons to develop direction-selective responses that maintain the requirement for joint spatial and temporal activation. We demonstrate that a novel form of inhibitory plasticity, postsynaptic activity-dependent long-term potentiation of inhibition (POSD-LTPi), which operates in the developing cortex at the time of eye opening, can provide synaptic competition and enables robust development of direction-selective receptive fields with unidirectional or bidirectional stimulation. We propose a general model of the development of spatiotemporal receptive fields that consists of two phases: an experience-independent establishment of initial biases, followed by an experience-dependent amplification or modification of these biases via correlation-based plasticity of excitatory inputs that compete against gradually increasing feed-forward inhibition. PMID:24598528

High Speed Research Program Structural Acoustics Multi-Year Summary Report

NASA Technical Reports Server (NTRS)

Beier, Theodor H.; Bhat, Waman V.; Rizzi, Stephen A.; Silcox, Richard J.; Simpson, Myles A.

2005-01-01

This report summarizes the work conducted by the Structural Acoustics Integrated Technology Development (ITD) Team under NASA's High Speed Research (HSR) Phase II program from 1993 to 1999. It is intended to serve as a reference for future researchers by documenting the results of the interior noise and sonic fatigue technology development activities conducted during this period. For interior noise, these activities included excitation modeling, structural acoustic response modeling, development of passive treatments and active controls, and prediction of interior noise. For sonic fatigue, these activities included loads prediction, materials characterization, sonic fatigue code development, development of response reduction techniques, and generation of sonic fatigue design requirements. Also included are lessons learned and recommendations for future work.

Development and Validation of Career Development Guidelines by Task/Activity Analysis of Occupational Safety and Health Professions: Industrial Hygiene and Safety Professional. Final Report. Technical Report XII.

ERIC Educational Resources Information Center

Vernon, Ralph J.; And Others

This report summarizes research findings which resulted in development of curricula for occupational safety and health professions based on task/activity analyses and related performance objectives. The first seven chapters focus on the seven objectives. Chapter 1, Literature Review and Selection of Employers, concerns tasks required for…

Ca2+ Requirement for Aerobic Nitrogen Fixation by Heterocystous Blue-Green Algae 1

PubMed Central

Rodríguez, Herminia; Rivas, Joaquín; Guerrero, Miguel G.; Losada, Manuel

1990-01-01

The requirement of Ca2+ for growth and nitrogen fixation has been investigated in two strains of heterocystous blue-green algae (Anabaena sp. and Anabaena ATCC 33047). With combined nitrogen (nitrate or ammonium) or with N2 under microaerobic conditions, Ca2+ was not required for growth, at least in concentrations greater than traces. In contrast, Ca2+ was required as a macronutrient for growth and nitrogen fixation with air as the nitrogen source. Addition of Ca2+ to an aerobic culture without Ca2+ promoted, after a lag of several hours, development of nitrogenase activity and cell growth. Provision of air to a microaerobic culture in the absence of Ca2+ promoted a drastic drop in nitrogenase activity, which rapidly recovered its initial level upon restoration of microaerobic conditions. Development of nitrogenase activity in response to either Ca2+ or low oxygen tension was dependent on de novo protein synthesis. The role of Ca2+ seems to be related to protection of nitrogenase from inactivation, by conferring heterocysts resistance to oxygen. PMID:16667401

Targeting Palmitoyl Acyltransferases in Mutant NRAS-Driven Melanoma

DTIC Science & Technology

2015-10-01

activation in melanoma cells using chemical biology and functional genomic approaches. In the first year of the study, we have developed more potent...post-translational modification by adding a 16-carbon palmitate) is required for N-RAS proper membrane localization and its oncogenic activities ...RAS regulation could be a novel strategy to treat N-RAS mutant melanoma. We have developed chemical probes that covalently label the active sites of

Space Tourism: Orbital Debris Considerations

NASA Astrophysics Data System (ADS)

Mahmoudian, N.; Shajiee, S.; Moghani, T.; Bahrami, M.

2002-01-01

Space activities after a phase of research and development, political competition and national prestige have entered an era of real commercialization. Remote sensing, earth observation, and communication are among the areas in which this growing industry is facing competition and declining government money. A project like International Space Station, which draws from public money, has not only opened a window of real multinational cooperation, but also changed space travel from a mere fantasy into a real world activity. Besides research activities for sending man to moon and Mars and other outer planets, space travel has attracted a considerable attention in recent years in the form of space tourism. Four countries from space fairing nations are actively involved in the development of space tourism. Even, nations which are either in early stages of space technology development or just beginning their space activities, have high ambitions in this area. This is worth noting considering their limited resources. At present, trips to space are available, but limited and expensive. To move beyond this point to generally available trips to orbit and week long stays in LEO, in orbital hotels, some of the required basic transportations, living requirements, and technological developments required for long stay in orbit are already underway. For tourism to develop to a real everyday business, not only the price has to come down to meaningful levels, but also safety considerations should be fully developed to attract travelers' trust. A serious hazard to space activities in general and space tourism in particular is space debris in earth orbit. Orbiting debris are man-made objects left over by space operations, hazardous to space missions. Since the higher density of debris population occurs in low earth orbit, which is also the same orbit of interest to space tourism, a careful attention should be paid to the effect of debris on tourism activities. In this study, after a review of the current work on space tourism and debris situation in low earth orbit suitable orbits for space tourism activities with regard to the presence of orbital debris are discussed.

Technology transfer program: Perspective

NASA Technical Reports Server (NTRS)

Toyshov, A. J.

1981-01-01

Most of NASA's technology transfer activities are in the area of land use (development, suitability, and planning); forestry (including wildlife and range and vegetation inventories) agriculture related activities; and water resources. The technology dissemination function is exercised through three regional applications centers which are involved in 91 applications projects within 22 states. In addition there are approximately eight application system verification transfer (ASVT) projects, 21 university applications branches, institutionalized liason activities with public interest groups, and user requirements activities. As the result of budget cuts, the ASVT and user requirements and awareness programs are to be phased out at the end of FY81. The university applications programs are to be phased down and terminated by 1985. NASA will continue to work with the user more in an R & D and an applications development capacity, and not in a national scale or administrative way.

Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases

PubMed Central

González-Vera, Juan A.; Morris, May C.

2015-01-01

Probing the dynamic activities of protein kinases in real-time in living cells constitutes a major challenge that requires specific and sensitive tools tailored to meet the particular demands associated with cellular imaging. The development of genetically-encoded and synthetic fluorescent biosensors has provided means of monitoring protein kinase activities in a non-invasive fashion in their native cellular environment with high spatial and temporal resolution. Here, we review existing technologies to probe different dynamic features of protein kinases and discuss limitations where new developments are required to implement more performant tools, in particular with respect to infrared and near-infrared fluorescent probes and strategies which enable improved signal-to-noise ratio and controlled activation of probes. PMID:28248276

TOPEX Project Radar Altimeter Development Requirements and Specifications, Version 6.0

NASA Technical Reports Server (NTRS)

Rossi, Laurence C.

2003-01-01

This document provides the guidelines by which the TOPEX Radar Altimeter hardware development effort for the TOPEX flight project shall be implemented and conducted. The conduct of this activity shall take maximum advantage of the efforts expended during the TOPEX Radar Altimeter Advanced Technology Model development program and other related Radar Altimeter development efforts. This document complies with the TOPEX Project Office document 633-420 (D-2218), entitled, "TOPEX Project Requirements and Constraints for the NASA Radar Altimeter" dated December 1987.

Study of onboard expert systems to augment space shuttle and space station autonomy

NASA Technical Reports Server (NTRS)

Kurtzman, C. R.; Akin, D. L.; Kranzler, D.; Erlanson, E.

1986-01-01

The feasibility of onboard crew activity planning was examined. The use of expert systems technology to aid crewmembers in locating stowed equipment was also investigated. The crew activity planning problem, along with a summary of past and current research efforts, was discussed in detail. The requirements and specifications used to develop the crew activity planning system was also defined. The guidelines used to create, develop, and operate the MFIVE Crew Scheduler and Logistics Clerk were discussed. Also discussed is the mathematical algorithm, used by the MFIVE Scheduler, which was developed to aid in optimal crew activity planning.

ASPECT: A Survey to Assess Student Perspective of Engagement in an Active-Learning Classroom

ERIC Educational Resources Information Center

Wiggins, Benjamin L.; Eddy, Sarah L.; Wener-Fligner, Leah; Freisem, Karen; Grunspan, Daniel Z.; Theobald, Elli J.; Timbrook, Jerry; Crowe, Alison J.

2017-01-01

The primary measure used to determine relative effectiveness of in-class activities has been student performance on pre/posttests. However, in today's active-learning classrooms, learning is a social activity, requiring students to interact and learn from their peers. To develop effective active-learning exercises that engage students, it is…

The SH2-containing tyrosine phosphatase corkscrew is required during signaling by sevenless, Ras1 and Raf.

PubMed

Allard, J D; Chang, H C; Herbst, R; McNeill, H; Simon, M A

1996-04-01

The sevenless gene encodes a receptor tyrosine kinase which is required for the development of the R7 photoreceptor cell in each ommatidium of the Drosophila eye. We have previously used a sensitized genetic screen to identify mutations, designated Enhancers of sevenless (E(sev)), which affect genes that encode components of the sevenless signaling pathway. Here, we report that one of these mutations, E(sev)1Ae0P is a dominantly inhibiting allele of corkscrew, which encodes an SH2 domain-containing protein tyrosine phosphatase (Perkins et al., 1992). We show that corkscrew function is essential for sevenless signaling and that expression of a membrane-targeted form of corkscrew can drive R7 photoreceptor development in the absence of sevenless function. Furthermore, we have used the dominantly inhibiting corkscrew allele to examine the role of corkscrew during signaling by activated forms of Ras1 and Raf. Our analysis indicates that corkscrew function is still required during signaling by activated forms Ras1 and Raf proteins. These results define a function for corkscrew that is either downstream of Ras1 activation or in a parallel pathway that acts with activated Ras1/Raf to specify R7 photoreceptor development.

Activation of the Arabidopsis B class homeotic genes by APETALA1.

PubMed

Ng, M; Yanofsky, M F

2001-04-01

Proper development of petals and stamens in Arabidopsis flowers requires the activities of APETALA3 (AP3) and PISTILLATA (PI), whose transcripts can be detected in the petal and stamen primordia. Localized expression of AP3 and PI requires the activities of at least three genes: APETALA1 (AP1), LEAFY (LFY), and UNUSUAL FLORAL ORGANS (UFO). It has been proposed that UFO provides spatial cues and that LFY specifies competence for AP3 and PI expression in the developing flower. To understand the epistatic relationship among AP1, LFY, and UFO in regulating AP3 and PI expression, we generated two versions of AP1 that have strong transcriptional activation potential. Genetic and molecular analyses of transgenic plants expressing these activated AP1 proteins show that the endogenous AP1 protein acts largely as a transcriptional activator in vivo and that AP1 specifies petals by regulating the spatial domains of AP3 and PI expression through UFO.

Visual Circuit Development Requires Patterned Activity Mediated by Retinal Acetylcholine Receptors

PubMed Central

Burbridge, Timothy J.; Xu, Hong-Ping; Ackman, James B.; Ge, Xinxin; Zhang, Yueyi; Ye, Mei-Jun; Zhou, Z. Jimmy; Xu, Jian; Contractor, Anis; Crair, Michael C.

2014-01-01

SUMMARY The elaboration of nascent synaptic connections into highly ordered neural circuits is an integral feature of the developing vertebrate nervous system. In sensory systems, patterned spontaneous activity before the onset of sensation is thought to influence this process, but this conclusion remains controversial largely due to the inherent difficulty recording neural activity in early development. Here, we describe novel genetic and pharmacological manipulations of spontaneous retinal activity, assayed in vivo, that demonstrate a causal link between retinal waves and visual circuit refinement. We also report a de-coupling of downstream activity in retinorecipient regions of the developing brain after retinal wave disruption. Significantly, we show that the spatiotemporal characteristics of retinal waves affect the development of specific visual circuits. These results conclusively establish retinal waves as necessary and instructive for circuit refinement in the developing nervous system and reveal how neural circuits adjust to altered patterns of activity prior to experience. PMID:25466916

Verification and accreditation schemes for climate change activities: A review of requirements for verification of greenhouse gas reductions and accreditation of verifiers—Implications for long-term carbon sequestration

NASA Astrophysics Data System (ADS)

Roed-Larsen, Trygve; Flach, Todd

The purpose of this chapter is to provide a review of existing national and international requirements for verification of greenhouse gas reductions and associated accreditation of independent verifiers. The credibility of results claimed to reduce or remove anthropogenic emissions of greenhouse gases (GHG) is of utmost importance for the success of emerging schemes to reduce such emissions. Requirements include transparency, accuracy, consistency, and completeness of the GHG data. The many independent verification processes that have developed recently now make up a quite elaborate tool kit for best practices. The UN Framework Convention for Climate Change and the Kyoto Protocol specifications for project mechanisms initiated this work, but other national and international actors also work intensely with these issues. One initiative gaining wide application is that taken by the World Business Council for Sustainable Development with the World Resources Institute to develop a "GHG Protocol" to assist companies in arranging for auditable monitoring and reporting processes of their GHG activities. A set of new international standards developed by the International Organization for Standardization (ISO) provides specifications for the quantification, monitoring, and reporting of company entity and project-based activities. The ISO is also developing specifications for recognizing independent GHG verifiers. This chapter covers this background with intent of providing a common understanding of all efforts undertaken in different parts of the world to secure the reliability of GHG emission reduction and removal activities. These verification schemes may provide valuable input to current efforts of securing a comprehensive, trustworthy, and robust framework for verification activities of CO2 capture, transport, and storage.

Development of an expert system prototype for determining software functional requirements for command management activities at NASA Goddard

NASA Technical Reports Server (NTRS)

Liebowitz, J.

1985-01-01

The development of an expert system prototype for determining software functional requirements for NASA Goddard's Command Management System (CMS) is described. The role of the CMS is to transform general requests into specific spacecraft commands with command execution conditions. The CMS is part of the NASA Data System which entails the downlink of science and engineering data from NASA near-earth satellites to the user, and the uplink of command and control data to the spacecraft. Subjects covered include: the problem environment of determining CMS software functional requirements; the expert system approach for handling CMS requirements development; validation and evaluation procedures for the expert system.

Pathfinder autonomous rendezvous and docking project

NASA Technical Reports Server (NTRS)

Lamkin, Stephen (Editor); Mccandless, Wayne (Editor)

1990-01-01

Capabilities are being developed and demonstrated to support manned and unmanned vehicle operations in lunar and planetary orbits. In this initial phase, primary emphasis is placed on definition of the system requirements for candidate Pathfinder mission applications and correlation of these system-level requirements with specific requirements. The FY-89 activities detailed are best characterized as foundation building. The majority of the efforts were dedicated to assessing the current state of the art, identifying desired elaborations and expansions to this level of development and charting a course that will realize the desired objectives in the future. Efforts are detailed across all work packages in developing those requirements and tools needed to test, refine, and validate basic autonomous rendezvous and docking elements.

Analysts guide to FEEMA for financial analysis of ecosystem management activities.

Treesearch

Roger D. Fight; John T. Chmelik

1998-01-01

This report describes strategies for using the Financial Evaluation of Ecosystem Management Activities (FEEMA) software. This program was developed as a tool for assessing the financial viability of management activities for removing small trees for manufacture into wood products. Combinations of tree stands, management activities, and contractual requirements can be...

Cardiovascular Endurance Activities for Children in Grades Four Through Six.

ERIC Educational Resources Information Center

Thompson, Mary Ann

A program of cardiovascular endurance activities for children in grades four through six was developed to emphasize success and improvement and establish lifelong patterns of concern for and enjoyment of activities that contribute to physical fitness and optimum health. The activities in the program require more teacher preparation than the…

Framework Requirements for MDO Application Development

NASA Technical Reports Server (NTRS)

Salas, A. O.; Townsend, J. C.

1999-01-01

Frameworks or problem solving environments that support application development form an active area of research. The Multidisciplinary Optimization Branch at NASA Langley Research Center is investigating frameworks for supporting multidisciplinary analysis and optimization research. The Branch has generated a list of framework requirements, based on the experience gained from the Framework for Interdisciplinary Design Optimization project and the information acquired during a framework evaluation process. In this study, four existing frameworks are examined against these requirements. The results of this examination suggest several topics for further framework research.

Spacelab user implementation assessment study. (Software requirements analysis). Volume 2: Technical report

NASA Technical Reports Server (NTRS)

1976-01-01

The engineering analyses and evaluation studies conducted for the Software Requirements Analysis are discussed. Included are the development of the study data base, synthesis of implementation approaches for software required by both mandatory onboard computer services and command/control functions, and identification and implementation of software for ground processing activities.

76 FR 71119 - Agency Information Collection Activity Under OMB Review

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-16

.... Abstract: 49 U.S.C. 5330 requires States to designate a State Safety Oversight (SSO) agency to oversee the... 5330, SSO agencies must develop program standards which meet FTA's minimum requirements. In the Program Standard, which must be approved by FTA, each SSO agency must require each rail transit agency in the State...

Curriculum Mapping in Higher Education: A Case Study and Proposed Content Scope and Sequence Mapping Tool

ERIC Educational Resources Information Center

Arafeh, Sousan

2016-01-01

Best practice in curriculum development and implementation requires that discipline-based standards or requirements embody both curricular and programme scopes and sequences. Ensuring these are present and aligned in course/programme content, activities and assessments to support student success requires formalised and systematised review and…

40 CFR 35.160 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-07-01

... also be eligible for funding under sections 104(b)(3) (Water Quality Cooperative Agreements and Wetlands Development Grants), 205(j)(2) (Water Quality Management Planning), and section 205(g) (State...) Associated program requirements. Program requirements for water quality planning and management activities...

The Systems Engineering Process for Human Support Technology Development

NASA Technical Reports Server (NTRS)

Jones, Harry

2005-01-01

Systems engineering is designing and optimizing systems. This paper reviews the systems engineering process and indicates how it can be applied in the development of advanced human support systems. Systems engineering develops the performance requirements, subsystem specifications, and detailed designs needed to construct a desired system. Systems design is difficult, requiring both art and science and balancing human and technical considerations. The essential systems engineering activity is trading off and compromising between competing objectives such as performance and cost, schedule and risk. Systems engineering is not a complete independent process. It usually supports a system development project. This review emphasizes the NASA project management process as described in NASA Procedural Requirement (NPR) 7120.5B. The process is a top down phased approach that includes the most fundamental activities of systems engineering - requirements definition, systems analysis, and design. NPR 7120.5B also requires projects to perform the engineering analyses needed to ensure that the system will operate correctly with regard to reliability, safety, risk, cost, and human factors. We review the system development project process, the standard systems engineering design methodology, and some of the specialized systems analysis techniques. We will discuss how they could apply to advanced human support systems development. The purpose of advanced systems development is not directly to supply human space flight hardware, but rather to provide superior candidate systems that will be selected for implementation by future missions. The most direct application of systems engineering is in guiding the development of prototype and flight experiment hardware. However, anticipatory systems engineering of possible future flight systems would be useful in identifying the most promising development projects.

The Development of Strategic Thinking: Learning to Impact Human Systems in a Youth Activism Program

ERIC Educational Resources Information Center

Larson, Reed; Hansen, David

2005-01-01

Human systems, including institutional systems and informal social networks, are a major arena of modern life. We argue that distinct forms of pragmatic reasoning or "strategic thinking" are required to exercise agency within such systems. This article explores the development of strategic thinking in a youth activism program in which young people…

Information Competence of a Library Specialist as a Condition for Their Professional Development

ERIC Educational Resources Information Center

Kaviev, Airat F.; Mamontova, Marina S.

2016-01-01

The relevance of the study is due to the intensive introduction of information technology to library activities. Active use of information technology has a significant impact on the professional development of a librarian. It requires a high level of information competence. The purpose of the paper is to present and describe an information…

Structurally well-defined macrophage activating factor derived from vitamin D3-binding protein has a potent adjuvant activity for immunization.

PubMed

Yamamoto, N; Naraparaju, V R

1998-06-01

Freund's adjuvant produced severe inflammation that augments development of antibodies. Thus, mixed administration of antigens with adjuvant was not required as long as inflammation was induced in the hosts. Since macrophage activation for phagocytosis and antigen processing is the first step of antibody development, inflammation-primed macrophage activation plays a major role in immune development. Therefore, macrophage activating factor should act as an adjuvant for immunization. The inflammation-primed macrophage activation process is the major macrophage activating cascade that requires participation of serum vitamin D3-binding protein (DBP; human DBP is known as Gc protein) and glycosidases of B and T lymphocytes. Stepwise incubation of Gc protein with immobilized beta-galactosidase and sialidase efficiently generated the most potent macrophage activating factor (designated GcMAF) we have ever encountered. Administration of GcMAF (20 or 100 pg/mouse) resulted in stimulation of the progenitor cells for extensive mitogenesis and activation of macrophages. Administration of GcMAF (100 pg/mouse) along with immunization of mice with sheep red blood cells (SRBC) produced a large number of anti-SRBC antibody secreting splenic cells in 2-4 days. Thus, GcMAF has a potent adjuvant activity for immunization. Although malignant tumours are poorly immunogenic, 4 days after GcMAF-primed immunization of mice with heat-killed Ehrlich ascites tumour cells, the ascites tumour was no longer transplantable in these mice.

Technology development for lunar base water recycling

NASA Technical Reports Server (NTRS)

Schultz, John R.; Sauer, Richard L.

1992-01-01

This paper will review previous and ongoing work in aerospace water recycling and identify research activities required to support development of a lunar base. The development of a water recycle system for use in the life support systems envisioned for a lunar base will require considerable research work. A review of previous work on aerospace water recycle systems indicates that more efficient physical and chemical processes are needed to reduce expendable and power requirements. Development work on biological processes that can be applied to microgravity and lunar environments also needs to be initiated. Biological processes are inherently more efficient than physical and chemical processes and may be used to minimize resupply and waste disposal requirements. Processes for recovering and recycling nutrients such as nitrogen, phosphorus, and sulfur also need to be developed to support plant growth units. The development of efficient water quality monitors to be used for process control and environmental monitoring also needs to be initiated.

SHARP's systems engineering challenge: rectifying integrated product team requirements with performance issues in an evolutionary spiral development acquisition

NASA Astrophysics Data System (ADS)

Kuehl, C. Stephen

2003-08-01

Completing its final development and early deployment on the Navy's multi-role aircraft, the F/A-18 E/F Super Hornet, the SHAred Reconnaissance Pod (SHARP) provides the war fighter with the latest digital tactical reconnaissance (TAC Recce) Electro-Optical/Infrared (EO/IR) sensor system. The SHARP program is an evolutionary acquisition that used a spiral development process across a prototype development phase tightly coupled into overlapping Engineering and Manufacturing Development (EMD) and Low Rate Initial Production (LRIP) phases. Under a tight budget environment with a highly compressed schedule, SHARP challenged traditional acquisition strategies and systems engineering (SE) processes. Adopting tailored state-of-the-art systems engineering process models allowd the SHARP program to overcome the technical knowledge transition challenges imposed by a compressed program schedule. The program's original goal was the deployment of digital TAC Recce mission capabilities to the fleet customer by summer of 2003. Hardware and software integration technical challenges resulted from requirements definition and analysis activities performed across a government-industry led Integrated Product Team (IPT) involving Navy engineering and test sites, Boeing, and RTSC-EPS (with its subcontracted hardware and government furnished equipment vendors). Requirements development from a bottoms-up approach was adopted using an electronic requirements capture environment to clarify and establish the SHARP EMD product baseline specifications as relevant technical data became available. Applying Earned-Value Management (EVM) against an Integrated Master Schedule (IMS) resulted in efficiently managing SE task assignments and product deliveries in a dynamically evolving customer requirements environment. Application of Six Sigma improvement methodologies resulted in the uncovering of root causes of errors in wiring interconnectivity drawings, pod manufacturing processes, and avionics requirements specifications. Utilizing the draft NAVAIR SE guideline handbook and the ANSI/EIA-632 standard: Processes for Engineering a System, a systems engineering tailored process approach was adopted for the accelerated SHARP EMD prgram. Tailoring SE processes in this accelerated product delivery environment provided unique opportunities to be technically creative in the establishment of a product performance baseline. This paper provides an historical overview of the systems engineering activities spanning the prototype phase through the EMD SHARP program phase, the performance requirement capture activities and refinement process challenges, and what SE process improvements can be applied to future SHARP-like programs adopting a compressed, evolutionary spiral development acquisition paradigm.

Effect of activated charcoal on callus growth and shoot organogenesis in tobacco. [Nicotiana tabacum

DOE Office of Scientific and Technical Information (OSTI.GOV)

Constantin, M.J.; Henke, R.R.; Mansur, M.A.

1977-01-01

Incorporating activated charcoal (AC) in culture media has been shown to affect growth and development of various organisms. Since AC stimulates the development of tobacco haploid plantlets from cultured anthers, research was conducted to determine the effect of activated charcoal on pith-derived callus growth and shoot development in Nicotiana tabacum cv. Wisconsin 38. Our results indicate that the hormones required for callus growth and shoot development in Wisconsin-38 tobacco are adsorbed by AC, thereby inhibiting callus growth and prohibiting shoot development. This effect was observed even when AC was removed from the medium by filtration prior to culturing the callus.

24 CFR 1000.520 - What are the purposes of HUD review?

Code of Federal Regulations, 2010 CFR

2010-04-01

... URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring, Oversight and Accountability... performance to determine whether the recipient: (a) Has carried out its eligible activities in a timely manner, has carried out its eligible activities and certifications in accordance with the requirements and the...

24 CFR 1000.520 - What are the purposes of HUD review?

Code of Federal Regulations, 2012 CFR

2012-04-01

... URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring, Oversight and Accountability... performance to determine whether the recipient: (a) Has carried out its eligible activities in a timely manner, has carried out its eligible activities and certifications in accordance with the requirements and the...

24 CFR 1000.520 - What are the purposes of HUD review?

Code of Federal Regulations, 2011 CFR

2011-04-01

... URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring, Oversight and Accountability... performance to determine whether the recipient: (a) Has carried out its eligible activities in a timely manner, has carried out its eligible activities and certifications in accordance with the requirements and the...

24 CFR 3.415 - Access to course offerings.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 3... any of its education program or activity separately on the basis of sex, or require or refuse...

24 CFR 3.415 - Access to course offerings.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 3... any of its education program or activity separately on the basis of sex, or require or refuse...

24 CFR 3.415 - Access to course offerings.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 3... any of its education program or activity separately on the basis of sex, or require or refuse...

24 CFR 3.415 - Access to course offerings.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 3... any of its education program or activity separately on the basis of sex, or require or refuse...

Software Program: Software Management Guidebook

NASA Technical Reports Server (NTRS)

1996-01-01

The purpose of this NASA Software Management Guidebook is twofold. First, this document defines the core products and activities required of NASA software projects. It defines life-cycle models and activity-related methods but acknowledges that no single life-cycle model is appropriate for all NASA software projects. It also acknowledges that the appropriate method for accomplishing a required activity depends on characteristics of the software project. Second, this guidebook provides specific guidance to software project managers and team leaders in selecting appropriate life cycles and methods to develop a tailored plan for a software engineering project.

48 CFR 732.402 - General.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 732.402 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Advance Payments 732.402 General. (a)-(d) [Reserved] (e)(1) U.S... organizations require the approval of the Head of the Contracting Activity, after consultation with the Mission...

48 CFR 732.402 - General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 732.402 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Advance Payments 732.402 General. (a)-(d) [Reserved] (e)(1) U.S... organizations require the approval of the Head of the Contracting Activity, after consultation with the Mission...

University Transformation: New Demands and Coping Strategies

ERIC Educational Resources Information Center

Chidindi, Joseph

2012-01-01

University education system is undergoing transformation requirements particularly in the developing world. The demands require adaptation to the prevailing circumstances hence the need for relevant strategies for universities. Issues such as institutional profiles, organizational structure and work integration, research activities, and funding…

A pharmacy course on leadership and leading change.

PubMed

Sorensen, Todd D; Traynor, Andrew P; Janke, Kristin K

2009-04-07

To develop and implement a course that develops pharmacy students' leadership skills and encourages them to become leaders within the profession. A leadership course series was offered to pharmacy students on 2 campuses. The series incorporated didactic, experiential, and self-directed learning activities, and focused on developing core leadership skills, self-awareness, and awareness of the process for leading change. Students reported increased knowledge and confidence in their ability to initiate and lead efforts for change. The learning activities students' valued most were the StrengthsFinder assessment (67% of students rated "very useful") and a Leadership Networking Partners (LNP) program (83% of students rated "very useful"). Teaching leadership skills poses a significant challenge in curriculum development and requires multifaceted course design elements that resonate with students and engage the practice community. Addressing these requirements results in a high level of student engagement and a desire to continue the development of leadership skills.

Molybdenum-base cermet fuel development

NASA Astrophysics Data System (ADS)

Pilger, James P.; Gurwell, William E.; Moss, Ronald W.; White, George D.; Seifert, David A.

Development of a multimegawatt (MMW) space nuclear power system requires identification and resolution of several technical feasibility issues before selecting one or more promising system concepts. Demonstration of reactor fuel fabrication technology is required for cermet-fueled reactor concepts. The MMW reactor fuel development activity at Pacific Northwest Laboratory (PNL) is focused on producing a molybdenum-matrix uranium-nitride (UN) fueled cermte. This cermet is to have a high matrix density (greater than or equal to 95 percent) for high strength and high thermal conductance coupled with a high particle (UN) porosity (approximately 25 percent) for retention of released fission gas at high burnup. Fabrication process development involves the use of porous TiN microspheres as surrogate fuel material until porous Un microspheres become available. Process development was conducted in the areas of microsphere synthesis, particle sealing/coating, and high-energy-rate forming (HERF) and the vacuum hot press consolidation techniques. This paper summarizes the status of these activities.

NASA's Microgravity Technology Report: Summary of Activities 1997

NASA Technical Reports Server (NTRS)

Woodard, Dan

1998-01-01

The purpose of the 1997 NASA Microgravity Technology Report is to update the Microgravity Research Program's technology development policy and to present and assess current technology related activities and requirements identified within its research and technology disciplines.

24 CFR 1000.530 - What corrective and remedial actions will HUD request or recommend to address performance...

Code of Federal Regulations, 2012 CFR

2012-04-01

..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring... for completing activities or complying with the requirements of this part; (3) Recommend that the recipient suspend, discontinue, or not incur costs for the affected activity; (4) Recommend that the...

24 CFR 1000.530 - What corrective and remedial actions will HUD request or recommend to address performance...

Code of Federal Regulations, 2010 CFR

2010-04-01

..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring... for completing activities or complying with the requirements of this part; (3) Recommend that the recipient suspend, discontinue, or not incur costs for the affected activity; (4) Recommend that the...

24 CFR 1000.530 - What corrective and remedial actions will HUD request or recommend to address performance...

Code of Federal Regulations, 2011 CFR

2011-04-01

..., DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT NATIVE AMERICAN HOUSING ACTIVITIES Recipient Monitoring... for completing activities or complying with the requirements of this part; (3) Recommend that the recipient suspend, discontinue, or not incur costs for the affected activity; (4) Recommend that the...

77 FR 38338 - Dairyland Power Cooperative; La Crosse Boiling Water Reactor Exemption From Certain Security...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-27

... used.'' In 10 CFR 73.55, entitled ``Requirements for physical protection of licensed activities in...-shutdown decommissioning activities report (PSDAR). The DPC has been conducting dismantlement and decommissioning activities. The DPC is developing an onsite independent spent fuel storage installation (ISFSI...

76 FR 73644 - Announcement of Requirements and Registration for the United States Surgeon General's Healthy...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-29

... enjoy health promoting behaviors related to fitness and physical activity, nutrition and healthy eating... develop software applications (apps) in the following categories: Fitness/physical activity: This category... promoting behaviors related to fitness and physical activity; nutrition/healthy eating; or physical and...

Therapeutic targeting of NOTCH1 signaling in T-ALL

PubMed Central

Palomero, Teresa; Ferrando, Adolfo

2010-01-01

The recent identification of activating mutations in NOTCH1 in the majority of T-cell acute lymphoblastic leukemias (T-ALL) has brought major interest towards targeting the NOTCH signaling pathway in this disease. Small molecule γ-secretase inhibitors (GSIs) which block a critical proteolytic step required for NOTCH1 activation can effectively block the activity of NOTCH1 mutant alleles. However, the clinical development of GSIs has been hampered by their low cytotoxicity against human T-ALL and the development of significant gastrointestinal toxicity derived from inhibition of NOTCH signaling in the gut. Improved understanding of the oncogenic mechanisms of NOTCH1 and the effects of NOTCH inhibition in leukemic cells and the intestinal epithelium are required for the design of effective anti-NOTCH1 therapies in T-ALL. PMID:19778842

Design, development, and fabrication of extravehicular activity tools for support of the transfer orbit stage

NASA Technical Reports Server (NTRS)

Albritton, L. M.; Redmon, J. W.; Tyler, T. R.

1993-01-01

Seven extravehicular activity (EVA) tools and a tool carrier have been designed and developed by MSFC in order to provide a two fault tolerant system for the transfer orbit stage (TOS) shuttle mission. The TOS is an upper stage booster for delivering payloads to orbits higher than the shuttle can achieve. Payloads are required not to endanger the shuttle even after two failures have occurred. The Airborne Support Equipment (ASE), used in restraining and deploying TOS, does not meet this criteria. The seven EVA tools designed will provide the required redundancy with no impact to the TOS hardware.

24 CFR 960.204 - Denial of admission for criminal activity or drug abuse by household members.

Code of Federal Regulations, 2012 CFR

2012-04-01

... activity or drug abuse by household members. 960.204 Section 960.204 Housing and Urban Development... HOUSING Admission § 960.204 Denial of admission for criminal activity or drug abuse by household members. (a) Required denial of admission—(1) Persons evicted for drug-related criminal activity. The PHA...

24 CFR 960.204 - Denial of admission for criminal activity or drug abuse by household members.

Code of Federal Regulations, 2014 CFR

2014-04-01

... activity or drug abuse by household members. 960.204 Section 960.204 Housing and Urban Development... HOUSING Admission § 960.204 Denial of admission for criminal activity or drug abuse by household members. (a) Required denial of admission—(1) Persons evicted for drug-related criminal activity. The PHA...

24 CFR 960.204 - Denial of admission for criminal activity or drug abuse by household members.

Code of Federal Regulations, 2011 CFR

2011-04-01

... activity or drug abuse by household members. 960.204 Section 960.204 Housing and Urban Development... HOUSING Admission § 960.204 Denial of admission for criminal activity or drug abuse by household members. (a) Required denial of admission—(1) Persons evicted for drug-related criminal activity. The PHA...

24 CFR 960.204 - Denial of admission for criminal activity or drug abuse by household members.

Code of Federal Regulations, 2010 CFR

2010-04-01

... activity or drug abuse by household members. 960.204 Section 960.204 Housing and Urban Development... HOUSING Admission § 960.204 Denial of admission for criminal activity or drug abuse by household members. (a) Required denial of admission—(1) Persons evicted for drug-related criminal activity. The PHA...

24 CFR 960.204 - Denial of admission for criminal activity or drug abuse by household members.

Code of Federal Regulations, 2013 CFR

2013-04-01

... activity or drug abuse by household members. 960.204 Section 960.204 Housing and Urban Development... HOUSING Admission § 960.204 Denial of admission for criminal activity or drug abuse by household members. (a) Required denial of admission—(1) Persons evicted for drug-related criminal activity. The PHA...

History of Mathematics: Three Activities to Use with Undergraduate Students and In-Service Teachers

ERIC Educational Resources Information Center

Loats, Jim; White, Diana; Rubino, Carmen

2014-01-01

We provide in-depth information and analysis on three activities for use in History of Mathematics courses taught either in a traditional semester format for undergraduates or in a summer professional development course for middle school teachers. These activities require students to be active participants in their own learning. They also…

34 CFR 200.60 - Expenditures for professional development.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 1 2011-07-01 2011-07-01 false Expenditures for professional development. 200.60... Paraprofessionals § 200.60 Expenditures for professional development. (a)(1) Except as provided in paragraph (a)(2... professional development activities to ensure that teachers and paraprofessionals meet the requirements of...

34 CFR 200.60 - Expenditures for professional development.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 1 2010-07-01 2010-07-01 false Expenditures for professional development. 200.60... Paraprofessionals § 200.60 Expenditures for professional development. (a)(1) Except as provided in paragraph (a)(2... professional development activities to ensure that teachers and paraprofessionals meet the requirements of...

75 FR 68305 - Proposed Rule Staying Numeric Limitation for the Construction and Development Point Source Category

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-05

... coverage and performing the following activities: Construction of buildings, including building, developing... Staying Numeric Limitation for the Construction and Development Point Source Category AGENCY... effluent limitation of 280 NTU and associated monitoring requirements for the Construction and Development...

A "Kanes's Dynamics" Model for the Active Rack Isolation System

NASA Technical Reports Server (NTRS)

Hampton, R. David; Beech, Geoffrey

1999-01-01

Many microgravity space-science experiments require vibratory acceleration levels unachievable without active isolation. The Boeing Corporation's Active Rack Isolation System (ARIS) employs a novel combination of magnetic actuation and mechanical linkages, to address these isolation requirements on the International Space Station (ISS). ARIS provides isolation at the rack (international Standard Payload Rack, or ISPR) level. Effective model-based vibration isolation requires (1) an appropriate isolation device, (2) an adequate dynamic (i.e., mathematical) model of that isolator, and (3) a suitable, corresponding controller. ARIS provides the ISS response to the first requirement. This paper presents one response to the second, in a state-space framework intended to facilitate an optimal-controls approach to the third. The authors use "Kane's Dynamics" to develop an state-space, analytical (algebraic) set of linearized equations of motion for ARIS.

A "Kane's Dynamics" Model for the Active Rack Isolation System

NASA Technical Reports Server (NTRS)

Hampton, R. D.; Beech, G. S.; Rao, N. N. S.; Rupert, J. K.; Kim, Y. K.

2001-01-01

Many microgravity space science experiments require vibratory acceleration levels unachievable without active isolation. The Boeing Corporation's Active Rack Isolation System (ARIS) employs a novel combination of magnetic actuation and mechanical linkages to address these isolation requirements on the International Space Station (ISS). ARIS provides isolation at the rack (International Standard Payload Rack (ISPR)) level. Effective model-based vibration isolation requires: (1) an appropriate isolation device, (2) an adequate dynamic (i.e., mathematical) model of that isolator, and (3) a suitable, corresponding controller. ARIS provides the ISS response to the first requirement. This paper presents one response to the second, in a state space framework intended to facilitate an optimal-controls approach to the third. The authors use "Kane's Dynamics" to develop a state-space, analytical (algebraic) set of linearized equations of motion for ARIS.

Orbital construction support equipment

NASA Technical Reports Server (NTRS)

1977-01-01

Approximately 200 separate construction steps were defined for the three solar power satellite (SPS) concepts. Detailed construction scenarios were developed which describe the specific tasks to be accomplished, and identify general equipment requirements. The scenarios were used to perform a functional analysis, which resulted in the definition of 100 distinct SPS elements. These elements are the components, parts, subsystems, or assemblies upon which construction activities take place. The major SPS elements for each configuration are shown. For those elements, 300 functional requirements were identified in seven generic processes. Cumulatively, these processes encompass all functions required during SPS construction/assembly. Individually each process is defined such that it includes a specific type of activity. Each SPS element may involve activities relating to any or all of the generic processes. The processes are listed, and examples of the requirements defined for a typical element are given.

Data Requirement (DR) MA-03: Payload missions integration. [Spacelab payloads

NASA Technical Reports Server (NTRS)

1985-01-01

Project management and payload integration requirements definition activities are reported. Mission peculiar equipment; systems integration; ground operations analysis and requirement definition; safety and quality assurance; and support systems development are examined for payloads planned for the following missions: EOM-1; SL-2; Sl-3 Astro-1; MSL-2; EASE/ACCESS; MPESS; and the middeck ADSF flight.

Active cell mechanics: Measurement and theory.

PubMed

Ahmed, Wylie W; Fodor, Étienne; Betz, Timo

2015-11-01

Living cells are active mechanical systems that are able to generate forces. Their structure and shape are primarily determined by biopolymer filaments and molecular motors that form the cytoskeleton. Active force generation requires constant consumption of energy to maintain the nonequilibrium activity to drive organization and transport processes necessary for their function. To understand this activity it is necessary to develop new approaches to probe the underlying physical processes. Active cell mechanics incorporates active molecular-scale force generation into the traditional framework of mechanics of materials. This review highlights recent experimental and theoretical developments towards understanding active cell mechanics. We focus primarily on intracellular mechanical measurements and theoretical advances utilizing the Langevin framework. These developing approaches allow a quantitative understanding of nonequilibrium mechanical activity in living cells. This article is part of a Special Issue entitled: Mechanobiology. Copyright © 2015. Published by Elsevier B.V.

Pollen-tube tip growth requires a balance of lateral propagation and global inhibition of Rho-family GTPase activity

PubMed Central

Hwang, Jae-Ung; Wu, Guang; Yan, An; Lee, Yong-Jik; Grierson, Claire S.; Yang, Zhenbiao

2010-01-01

Rapid tip growth allows for efficient development of highly elongated cells (e.g. neuronal axons, fungal hyphae and pollen tubes) and requires an elaborate spatiotemporal regulation of the growing region. Here, we use the pollen tube as a model to investigate the mechanism regulating the growing region. ROPs (Rho-related GTPases from plants) are essential for pollen tip growth and display oscillatory activity changes in the apical plasma membrane (PM). By manipulating the ROP activity level, we showed that the PM distribution of ROP activity as an apical cap determines the tip growth region and that efficient tip growth requires an optimum level of the apical ROP1 activity. Excessive ROP activation induced the enlargement of the tip growth region, causing growth depolarization and reduced tube elongation. Time-lapse analysis suggests that the apical ROP1 cap is generated by lateral propagation of a localized ROP activity. Subcellular localization and gain- and loss-of-function analyses suggest that RhoGDI- and RhoGAP-mediated global inhibition limits the lateral propagation of apical ROP1 activity. We propose that the balance between the lateral propagation and the global inhibition maintains an optimal apical ROP1 cap and generates the apical ROP1 activity oscillation required for efficient pollen-tube elongation. PMID:20053639

Learning challenges and sustainable development: A methodological perspective.

PubMed

Seppänen, Laura

2017-01-01

Sustainable development requires learning, but the contents of learning are often complex and ambiguous. This requires new integrated approaches from research. It is argued that investigation of people's learning challenges in every-day work is beneficial for research on sustainable development. The aim of the paper is to describe a research method for examining learning challenges in promoting sustainable development. This method is illustrated with a case example from organic vegetable farming in Finland. The method, based on Activity Theory, combines historical analysis with qualitative analysis of need expressions in discourse data. The method linking local and subjective need expressions with general historical analysis is a promising way to overcome the gap between the individual and society, so much needed in research for sustainable development. Dialectically informed historical frameworks have practical value as tools in collaborative negotiations and participatory designs for sustainable development. The simultaneous use of systemic and subjective perspectives allows researchers to manage the complexity of practical work activities and to avoid too simplistic presumptions about sustainable development.

76 FR 21744 - Agency Information Collection Activities: Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-18

... outcomes and (1) is assembled or developed by a provider for reporting to a PSO and is reported to a PSO or (2) is developed by a PSO for the conduct of patient safety activities. Civil money penalties may be... be listed as a PSO by the Secretary must certify that it meets certain requirements and, upon listing...

Differential effects of Rho GTPases on axonal and dendritic development in hippocampal neurones.

PubMed

Ahnert-Hilger, G; Höltje, M; Grosse, G; Pickert, G; Mucke, C; Nixdorf-Bergweiler, B; Boquet, P; Hofmann, F; Just, I

2004-07-01

Formation of neurites and their differentiation into axons and dendrites requires precisely controlled changes in the cytoskeleton. While small GTPases of the Rho family appear to be involved in this regulation, it is still unclear how Rho function affects axonal and dendritic growth during development. Using hippocampal neurones at defined states of differentiation, we have dissected the function of RhoA in axonal and dendritic growth. Expression of a dominant negative RhoA variant inhibited axonal growth, whereas dendritic growth was promoted. The opposite phenotype was observed when a constitutively active RhoA variant was expressed. Inactivation of Rho by C3-catalysed ADP-ribosylation using C3 isoforms (Clostridium limosum, C3(lim) or Staphylococcus aureus, C3(stau2)), diminished axonal branching. By contrast, extracellularly applied nanomolar concentrations of C3 from C. botulinum (C3(bot)) or enzymatically dead C3(bot) significantly increased axon growth and axon branching. Taken together, axonal development requires activation of RhoA, whereas dendritic development benefits from its inactivation. However, extracellular application of enzymatically active or dead C3(bot) exclusively promotes axonal growth and branching suggesting a novel neurotrophic function of C3 that is independent from its enzymatic activity.

4(1H)-Pyridone and 4(1H)-Quinolone Derivatives as Antimalarials with Erythrocytic, Exoerythrocytic, and Transmission Blocking Activities

PubMed Central

Monastyrskyi, Andrii; Kyle, Dennis E.; Manetsch, Roman

2015-01-01

Infectious diseases are the second leading cause of deaths in the world with malaria being responsible for approximately the same amount of deaths as cancer in 2012. Despite the success in malaria prevention and control measures decreasing the disease mortality rate by 45% since 2000, the development of single-dose therapeutics with radical cure potential is required to completely eradicate this deadly condition. Targeting multiple stages of the malaria parasite is becoming a primary requirement for new candidates in antimalarial drug discovery and development. Recently, 4(1H)-pyridone, 4(1H)-quinolone, 1,2,3,4-tetrahydroacridone, and phenoxyethoxy-4(1H)-quinolone chemotypes have been shown to be antimalarials with blood stage activity, liver stage activity, and transmission blocking activity. Advancements in structure-activity relationship and structure-property relationship studies, biological evaluation in vitro and in vivo, as well as pharmacokinetics of the 4(1H)-pyridone and 4(1H)-quinolone chemotypes will be discussed. PMID:25116582

Operations Assessment of Launch Vehicle Architectures using Activity Based Cost Models

NASA Technical Reports Server (NTRS)

Ruiz-Torres, Alex J.; McCleskey, Carey

2000-01-01

The growing emphasis on affordability for space transportation systems requires the assessment of new space vehicles for all life cycle activities, from design and development, through manufacturing and operations. This paper addresses the operational assessment of launch vehicles, focusing on modeling the ground support requirements of a vehicle architecture, and estimating the resulting costs and flight rate. This paper proposes the use of Activity Based Costing (ABC) modeling for this assessment. The model uses expert knowledge to determine the activities, the activity times and the activity costs based on vehicle design characteristics. The approach provides several advantages to current approaches to vehicle architecture assessment including easier validation and allowing vehicle designers to understand the cost and cycle time drivers.

Expert system for on-board satellite scheduling and control

NASA Technical Reports Server (NTRS)

Barry, John M.; Sary, Charisse

1988-01-01

An Expert System is described which Rockwell Satellite and Space Electronics Division (S&SED) is developing to dynamically schedule the allocation of on-board satellite resources and activities. This expert system is the Satellite Controller. The resources to be scheduled include power, propellant and recording tape. The activities controlled include scheduling satellite functions such as sensor checkout and operation. The scheduling of these resources and activities is presently a labor intensive and time consuming ground operations task. Developing a schedule requires extensive knowledge of the system and subsystems operations, operational constraints, and satellite design and configuration. This scheduling process requires highly trained experts anywhere from several hours to several weeks to accomplish. The process is done through brute force, that is examining cryptic mnemonic data off line to interpret the health and status of the satellite. Then schedules are formulated either as the result of practical operator experience or heuristics - that is rules of thumb. Orbital operations must become more productive in the future to reduce life cycle costs and decrease dependence on ground control. This reduction is required to increase autonomy and survivability of future systems. The design of future satellites require that the scheduling function be transferred from ground to on board systems.

30 CFR 285.436 - Can MMS require lease or grant contraction?

Code of Federal Regulations, 2010 CFR

2010-07-01

... lease or grant area is larger than needed to develop the project and manage activities in a manner that... information demonstrating that you need the area in question to manage lease or grant activities consistent...

Enterprise Professional Development--Evaluating Learning

ERIC Educational Resources Information Center

Murphy, Gerald A.; Calway, Bruce A.

2010-01-01

Whilst professional development (PD) is an activity required by many regulatory authorities, the value that enterprises obtain from PD is often unknown, particularly when it involves development of knowledge. This paper discusses measurement techniques and processes and provides a review of established evaluation techniques, highlighting…

Possible Brain Mechanisms of Creativity.

PubMed

Heilman, Kenneth M

2016-06-01

Creativity is the new discovery, understanding, development and expression of orderly and meaningful relationships. Creativity has three major stages: preparation, the development (nature and nurture) of critical knowledge and skills; innovation, the development of a creative solution; and creative production. Successful preparation requires a basic level of general intelligence and domain specific knowledge and skills and highly creative people may have anatomic alterations of specific neocortical regions. Innovation requires disengagement and divergent thinking primarily mediated by frontal networks. Creative people are often risk-takers and novelty seekers, behaviors that activate their ventral striatal reward system. Innovation also requires associative and convergent thinking, activities that are dependent on the integration of highly distributed networks. People are often most creative when they are in mental states associated with reduced levels of brain norepinephrine, which may enhance the communication between distributed networks. We, however, need to learn more about the brain mechanisms of creativity. Published by Oxford University Press 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.

The Him gene reveals a balance of inputs controlling muscle differentiation in Drosophila.

PubMed

Liotta, David; Han, Jun; Elgar, Stuart; Garvey, Clare; Han, Zhe; Taylor, Michael V

2007-08-21

Tissue development requires the controlled regulation of cell-differentiation programs. In muscle, the Mef2 transcription factor binds to and activates the expression of many genes and has a major positive role in the orchestration of differentiation. However, little is known about how Mef2 activity is regulated in vivo during development. Here, we characterize a gene, Holes in muscle (Him), which our results indicate is part of this control in Drosophila. Him expression rapidly declines as embryonic muscle differentiates, and consistent with this, Him overexpression inhibits muscle differentiation. This inhibitory effect is suppressed by mef2, implicating Him in the mef2 pathway. We then found that Him downregulates the transcriptional activity of Mef2 in both cell culture and in vivo. Furthermore, Him protein binds Groucho, a conserved, transcriptional corepressor, through a WRPW motif and requires this motif and groucho function to inhibit both muscle differentiation and Mef2 activity during development. Together, our results identify a mechanism that can inhibit muscle differentiation in vivo. We conclude that a balance of positive and negative inputs, including Mef2, Him, and Groucho, controls muscle differentiation during Drosophila development and suggest that one outcome is to hold developing muscle cells in a state with differentiation genes poised to be expressed.

The Him Gene Reveals a Balance of Inputs Controlling Muscle Differentiation in Drosophila

PubMed Central

Liotta, David; Han, Jun; Elgar, Stuart; Garvey, Clare; Han, Zhe; Taylor, Michael V.

2007-01-01

Summary Tissue development requires the controlled regulation of cell-differentiation programs. In muscle, the Mef2 transcription factor binds to and activates the expression of many genes and has a major positive role in the orchestration of differentiation [1–4]. However, little is known about how Mef2 activity is regulated in vivo during development. Here, we characterize a gene, Holes in muscle (Him), which our results indicate is part of this control in Drosophila. Him expression rapidly declines as embryonic muscle differentiates, and consistent with this, Him overexpression inhibits muscle differentiation. This inhibitory effect is suppressed by mef2, implicating Him in the mef2 pathway. We then found that Him downregulates the transcriptional activity of Mef2 in both cell culture and in vivo. Furthermore, Him protein binds Groucho, a conserved, transcriptional corepressor, through a WRPW motif and requires this motif and groucho function to inhibit both muscle differentiation and Mef2 activity during development. Together, our results identify a mechanism that can inhibit muscle differentiation in vivo. We conclude that a balance of positive and negative inputs, including Mef2, Him, and Groucho, controls muscle differentiation during Drosophila development and suggest that one outcome is to hold developing muscle cells in a state with differentiation genes poised to be expressed. PMID:17702578

The conception, birth, and growth of a missile umbilical system

NASA Technical Reports Server (NTRS)

Nordman, G. W.

1977-01-01

The design development was traced for the sprint 2 and improved sprint 2 missile system (ISMS) umbilical system. The unique system requirements, umbilical designs considered to meet the requirements, and the problems encountered and solutions derived during the design, and development testing of the selected systems are described. The sprint 2 development effort consisted of design, analysis, and testing activities. The ISMS effort involved the performance of an extensive trade study to determine the optimum design to meet the ISMS conditions.

Ternary complex factor SAP-1 is required for Erk-mediated thymocyte positive selection.

PubMed

Costello, Patrick S; Nicolas, Robert H; Watanabe, Yasuyuki; Rosewell, Ian; Treisman, Richard

2004-03-01

Thymocyte selection and differentiation requires extracellular signal-regulated kinase (Erk) signaling, but transcription factor substrates of Erk in thymocytes are unknown. We have characterized the function of SAP-1 (Elk4), an Erk-regulated transcription factor, in thymocyte development. Early thymocyte development was normal, but single-positive thymocyte and peripheral T cell numbers were reduced, reflecting a T cell-autonomous defect. T cell receptor-induced activation of SAP-1 target genes such as Egr1 was substantially impaired in double-positive thymocytes, although Erk activation was normal. Analysis of T cell receptor transgenes showed that positive selection was reduced by 80-90% in SAP-1-deficient mice; heterozygous mice showed a moderate defect. Negative selection was unimpaired. SAP-1 thus directly links Erk signaling to the transcriptional events required for thymocyte positive selection.

Leadership roles, competencies, and education: how prepared are our nurse managers?

PubMed

Kleinman, Carol S

2003-09-01

Although they are responsible for the operation of business units, nurse managers are often less well prepared to manage the business activities than the clinical activities. Perceptions of nurse managers and nurse executives regarding competencies required for nursing management roles and the educational preparation required to attain them were examined. Results indicate the groups are in basic agreement about required competencies, though nurse managers appear less clear about nurse executive role responsibilities. Nurse executives value the acquisition of a master's degree as essential for nurse manager performance, while fewer nurse managers agree. Strategies nurse executives may employ to develop nurse manager business knowledge include traditional undergraduate and graduate degree programs, online programs, certificate programs, continuing education, inservice education offerings, seminars, and mentoring activities.

Visit from JAXA to NASA MSFC: The Engines Element & Ideas for Collaboration

NASA Technical Reports Server (NTRS)

Greene, William D.

2013-01-01

System Design, Development, and Fabrication: Design, develop, and fabricate or procure MB-60 component hardware compliant with the imposed technical requirements and in sufficient quantities to fulfill the overall MB-60 development effort. System Development, Assembly, and Test: Manage the scope of the development, assembly, and test-related activities for MB-60 development. This scope includes engine-level development planning, engine assembly and disassembly, test planning, engine testing, inspection, anomaly resolution, and development of necessary ground support equipment and special test equipment. System Integration: Provide coordinated integration in the realms of engineering, safety, quality, and manufacturing disciplines across the scope of the MB-60 design and associated products development Safety and Mission Assurance, structural design, fracture control, materials and processes, thermal analysis. Systems Engineering and Analysis: Manage and perform Systems Engineering and Analysis to provide rigor and structure to the overall design and development effort for the MB-60. Milestone reviews, requirements management, system analysis, program management support Program Management: Manage, plan, and coordinate the activities across all portions of the MB-60 work scope by providing direction for program administration, business management, and supplier management.

Localised JAK/STAT Pathway Activation Is Required for Drosophila Wing Hinge Development

PubMed Central

Johnstone, Kirsty; Wells, Richard E.; Strutt, David; Zeidler, Martin P.

2013-01-01

Extensive morphogenetic remodelling takes place during metamorphosis from a larval to an adult insect body plan. These changes are particularly intricate in the generation of the dipteran wing hinge, a complex structure that is derived from an apparently simple region of the wing imaginal disc. Using the characterisation of original outstretched alleles of the unpaired locus as a starting point, we demonstrate the role of JAK/STAT pathway signalling in the process of wing hinge development. We show that differences in JAK/STAT signalling within the proximal most of three lateral folds present in the wing imaginal disc is required for fold morphology and the subsequent differentiation of the first and second auxiliary sclerites as well as the posterior notal wing process. Changes in these domains are consistent with the established fate map of the wing disc. We show that outstretched wing posture phenotypes arise from the loss of a region of Unpaired expression in the proximal wing fold and demonstrate that this results in a decrease in JAK/STAT pathway activity. Finally we show that reduction of JAK/STAT pathway activity within the proximal wing fold is sufficient to phenocopy the outstretched phenotype. Taken together, we suggest that localised Unpaired expression and hence JAK/STAT pathway activity, is required for the morphogenesis of the adult wing hinge, providing new insights into the link between signal transduction pathways, patterning and development. PMID:23741461

Solar Sail Models and Test Measurements Correspondence for Validation Requirements Definition

NASA Technical Reports Server (NTRS)

Ewing, Anthony; Adams, Charles

2004-01-01

Solar sails are being developed as a mission-enabling technology in support of future NASA science missions. Current efforts have advanced solar sail technology sufficient to justify a flight validation program. A primary objective of this activity is to test and validate solar sail models that are currently under development so that they may be used with confidence in future science mission development (e.g., scalable to larger sails). Both system and model validation requirements must be defined early in the program to guide design cycles and to ensure that relevant and sufficient test data will be obtained to conduct model validation to the level required. A process of model identification, model input/output documentation, model sensitivity analyses, and test measurement correspondence is required so that decisions can be made to satisfy validation requirements within program constraints.

Regulatory requirements for genotoxicity assessment of plant protection product active ingredients, impurities, and metabolites.

PubMed

Booth, Ewan D; Rawlinson, Paul J; Maria Fagundes, Priscila; Leiner, Kevin A

2017-06-01

Active ingredients in plant protection products are subject to rigorous safety assessment during their development, including assessment of genotoxicity. Plant protection products are used for agriculture in multiple regions and for the registration of active ingredients it is necessary to satisfy the data requirements of these different regions. There are no overarching global agreements on which genotoxicity studies need to be conducted to satisfy the majority of regulatory authorities. The implementation of new OECD guidelines for the in vitro micronucleus, transgenic rodent somatic and germ cell gene mutation and in vivo comet assays, as well as the revision of a number of other OECD test guidelines has resulted in some changes to data requirements. This review describes the genotoxicity data requirements for chemical active ingredients as well as biologicals, microbials, ground water metabolites, metabolites, and impurities in a number of regions. Similarities and differences are highlighted. Environ. Mol. Mutagen. 58:325-344, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Development of a Self Aligned CMOS Process for Flash Lamp Annealed Polycrystalline Silicon TFTs

NASA Astrophysics Data System (ADS)

Bischoff, Paul

The emerging active matrix liquid crystal (AMLCD) display market requires a high performing semiconductor material to meet rising standards of operation. Currently amorphous silicon (a-Si) dominates the market but it does not have the required mobility for it to be used in AMLCD manufacturing. Other materials have been developed including crystallizing a-Si into poly-silicon. A new approach to crystallization through the use of flash lamp annealing (FLA) decreases manufacturing time and greatly improves carrier mobility. Previous work on FLA silicon for the use in CMOS transistors revealed significant lateral dopant diffusion into the channel greatly increasing the minimum channel length required for a working device. This was further confounded by the gate overlap due to misalignment during lithography patterning steps. Through the use of furnace dopant activation instead of FLA dopant activation and a self aligned gate the minimum size transistor can be greatly reduced. A new lithography mask and process flow were developed for the furnace annealing and self aligned gate. Fabrication of the self aligned devices resulted in oxidation of the Molybdenum self aligned gate. Further development is needed to successfully manufacture these devices. Non-self aligned transistors were made simultaneously with self aligned devices and used the furnace activation. These devices showed an increase in sheet resistance from 250 O to 800 O and lower mobility from 380 to 40.2 V/cm2s. The lower mobility can be contributed to an increase in implanted trap density indicating furnace annealing is an inferior activation method over FLA. The minimum transistor size however was reduced from 20 to 5 mum. With improvements in the self aligned process high performing small devices can be manufactured.

Food systems for space travel.

PubMed

Bourland, C T

1999-01-01

Space food systems have evolved from tubes and cubes to Earth-like food being planned for the International Space Station. The weight, volume, and oxygen-enriched atmosphere constraints of earlier spacecraft severely limited the type of food that could be used. Food systems improved as spacecraft conditions became more habitable. Space food systems have traditionally been based upon the water supply. This presentation summarizes the food development activities from Mercury through Shuttle, Shuttle/Mir, and plans for the International Space Station. Food development lessons learned from the long-duration missions with astronauts on the Mir station are also discussed. Nutritional requirements for long-duration missions in microgravity and problems associated with meeting these requirements for Mir will be elucidated. The psychological importance of food and the implications for food development activities are summarized.

Research and Development, Technology Requirements, and Use of Existing Space Assets

NASA Technical Reports Server (NTRS)

Grey, Jerry; Rowell, Larry

1999-01-01

Technology requirements are addressed in three categories: (1) Passenger-carrying STS(s); (2) orbital facilities (including on-orbit operations such as servicing) and human factors (crew and passenger training and recreation, on-orbit environmental control, etc.); and (3) ground infrastructure. An example tourism trip scenario is used in discussing possible targets for technology development activities. Some general comments are made as are considerations for other working groups.

Laser-based firing systems for prompt initiation of secondary explosives

NASA Technical Reports Server (NTRS)

Meeks, Kent D.; Setchell, Robert E.

1993-01-01

Motivated by issues of weapon safety and security, laser based firing systems for promptly initiating secondary explosives have been under active development at Sandia National Laboratories for more than four years. Such a firing system consists of miniaturized, Q-switched, solid-state laser, optical detonators, optical safety switches, and elements for splitting, coupling, and transmitting the laser output. Potential system applications pose significant challenges in terms of server mechanical and thermal environments and packaging constraints, while requiring clear demonstration of safety enhancements. The Direct Optical Initiation (DOI) Program at Sandia is addressing these challenges through progress development phases during which the design, fabrication, and testing of prototype hardware is aimed at more difficult application requirements. A brief history of the development program, and a summary of current and planned activities, will be presented.

Foundational Report Series: Advanced Distribution Management Systems for Grid Modernization, High-Level Use Cases for DMS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Jianhui; Lu, Xiaonan; Martino, Sal

Many distribution management systems (DMS) projects have achieved limited success because the electric utility did not sufficiently plan for actual use of the DMS functions in the control room environment. As a result, end users were not clear on how to use the new application software in actual production environments with existing, well-established business processes. An important first step in the DMS implementation process is development and refinement of the “to be” business processes. Development of use cases for the required DMS application functions is a key activity that leads to the formulation of the “to be” requirements. It ismore » also an important activity that is needed to develop specifications that are used to procure a new DMS.« less

Requirements management for Gemini Observatory: a small organization with big development projects

NASA Astrophysics Data System (ADS)

Close, Madeline; Serio, Andrew; Cordova, Martin; Hardie, Kayla

2016-08-01

Gemini Observatory is an astronomical observatory operating two premier 8m-class telescopes, one in each hemisphere. As an operational facility, a majority of Gemini's resources are spent on operations however the observatory undertakes major development projects as well. Current projects include new facility science instruments, an operational paradigm shift to full remote operations, and new operations tools for planning, configuration and change control. Three years ago, Gemini determined that a specialized requirements management tool was needed. Over the next year, the Gemini Systems Engineering Group investigated several tools, selected one for a trial period and configured it for use. Configuration activities including definition of systems engineering processes, development of a requirements framework, and assignment of project roles to tool roles. Test projects were implemented in the tool. At the conclusion of the trial, the group determined that the Gemini could meet its requirements management needs without use of a specialized requirements management tool, and the group identified a number of lessons learned which are described in the last major section of this paper. These lessons learned include how to conduct an organizational needs analysis prior to pursuing a tool; caveats concerning tool criteria and the selection process; the prerequisites and sequence of activities necessary to achieve an optimum configuration of the tool; the need for adequate staff resources and staff training; and a special note regarding organizations in transition and archiving of requirements.

Development of molecular electrocatalysts for CO2 reduction and H2 production/oxidation.

PubMed

Rakowski DuBois, M; DuBois, Daniel L

2009-12-21

The conversion of solar energy to fuels in both natural and artificial photosynthesis requires components for both light-harvesting and catalysis. The light-harvesting component generates the electrochemical potentials required to drive fuel-generating reactions that would otherwise be thermodynamically uphill. This Account focuses on work from our laboratories on developing molecular electrocatalysts for CO(2) reduction and for hydrogen production. A true analog of natural photosynthesis will require the ability to capture CO(2) from the atmosphere and reduce it to a useful fuel. Work in our laboratories has focused on both aspects of this problem. Organic compounds such as quinones and inorganic metal complexes can serve as redox-active CO(2) carriers for concentrating CO(2). We have developed catalysts for CO(2) reduction to form CO based on a [Pd(triphosphine)(solvent)](2+) platform. Catalytic activity requires the presence of a weakly coordinating solvent molecule that can dissociate during the catalytic cycle and provide a vacant coordination site for binding water and assisting C-O bond cleavage. Structures of [NiFe] CO dehydrogenase enzymes and the results of studies on complexes containing two [Pd(triphosphine)(solvent)](2+) units suggest that participation of a second metal in CO(2) binding may also be required for achieving very active catalysts. We also describe molecular electrocatalysts for H(2) production and oxidation based on [Ni(diphosphine)(2)](2+) complexes. Similar to palladium CO(2) reduction catalysts, these species require the optimization of both first and second coordination spheres. In this case, we use structural features of the first coordination sphere to optimize the hydride acceptor ability of nickel needed to achieve heterolytic cleavage of H(2). We use the second coordination sphere to incorporate pendant bases that assist in a number of important functions including H(2) binding, H(2) cleavage, and the transfer of protons between nickel and solution. These pendant bases, or proton relays, are likely to be important in the design of catalysts for a wide range of fuel production and fuel utilization reactions involving multiple electron and proton transfer steps. The generation of fuels from abundant substrates such as CO(2) and water remains a daunting research challenge, requiring significant advances in new inexpensive materials for light harvesting and the development of fast, stable, and efficient electrocatalysts. Although we describe progress in the development of redox-active carriers capable of concentrating CO(2) and molecular electrocatalysts for CO(2) reduction, hydrogen production, and hydrogen oxidation, much more remains to be done.

Advanced Extra-Vehicular Activity Pressure Garment Requirements Development

NASA Technical Reports Server (NTRS)

Ross, Amy; Aitchison, Lindsay; Rhodes, Richard

2015-01-01

The NASA Johnson Space Center advanced pressure garment technology development team is addressing requirements development for exploration missions. Lessons learned from the Z-2 high fidelity prototype development have reiterated that clear low-level requirements and verification methods reduce risk to the government, improve efficiency in pressure garment design efforts, and enable the government to be a smart buyer. The expectation is to provide requirements at the specification level that are validated so that their impact on pressure garment design is understood. Additionally, the team will provide defined verification protocols for the requirements. However, in reviewing exploration space suit high level requirements there are several gaps in the team's ability to define and verify related lower level requirements. This paper addresses the efforts in requirement areas such as mobility/fit/comfort and environmental protection (dust, radiation, plasma, secondary impacts) to determine the method by which the requirements can be defined and use of those methods for verification. Gaps exist at various stages. In some cases component level work is underway, but no system level effort has begun; in other cases no effort has been initiated to close the gap. Status of on-going efforts and potential approaches to open gaps are discussed.

A model for the electronic support of practice-based research networks.

PubMed

Peterson, Kevin A; Delaney, Brendan C; Arvanitis, Theodoros N; Taweel, Adel; Sandberg, Elisabeth A; Speedie, Stuart; Richard Hobbs, F D

2012-01-01

The principal goal of the electronic Primary Care Research Network (ePCRN) is to enable the development of an electronic infrastructure to support clinical research activities in primary care practice-based research networks (PBRNs). We describe the model that the ePCRN developed to enhance the growth and to expand the reach of PBRN research. Use cases and activity diagrams were developed from interviews with key informants from 11 PBRNs from the United States and United Kingdom. Discrete functions were identified and aggregated into logical components. Interaction diagrams were created, and an overall composite diagram was constructed describing the proposed software behavior. Software for each component was written and aggregated, and the resulting prototype application was pilot tested for feasibility. A practical model was then created by separating application activities into distinct software packages based on existing PBRN business rules, hardware requirements, network requirements, and security concerns. We present an information architecture that provides for essential interactions, activities, data flows, and structural elements necessary for providing support for PBRN translational research activities. The model describes research information exchange between investigators and clusters of independent data sites supported by a contracted research director. The model was designed to support recruitment for clinical trials, collection of aggregated anonymous data, and retrieval of identifiable data from previously consented patients across hundreds of practices. The proposed model advances our understanding of the fundamental roles and activities of PBRNs and defines the information exchange commonly used by PBRNs to successfully engage community health care clinicians in translational research activities. By describing the network architecture in a language familiar to that used by software developers, the model provides an important foundation for the development of electronic support for essential PBRN research activities.

Observations of the Performance of the U.S. Laboratory Architecture

NASA Technical Reports Server (NTRS)

Jones, Rod

2002-01-01

The United States Laboratory Module "Destiny" was the product of many architectural, technology, manufacturing, schedule and cost constraints which spanned 15 years. Requirements for the Space Station pressurized elements were developed and baselined in the mid to late '80's. Although the station program went through several design changes the fundamental requirements that drove the architecture did not change. Manufacturing of the U.S. Laboratory began in the early 90's. Final assembly and checkout testing completed in December of 2000. Destiny was launched, mated to the International Space Station and successfully activated on the STS-98 mission in February of 2001. The purpose of this paper is to identify key requirements, which directly or indirectly established the architecture of the U.S. Laboratory. Provide an overview of how that architecture affected the manufacture, assembly, test, and activation of the module on-orbit. And finally, through observations made during the last year of operation, provide considerations in the development of future requirements and mission integration controls for space habitats.

System modeling of the Thirty Meter Telescope alignment and phasing system

NASA Astrophysics Data System (ADS)

Dekens, Frank G.; Seo, Byoung-Joon; Troy, Mitchell

2014-08-01

We have developed a system model using the System Modeling Language (SysML) for the Alignment and Phasing System (APS) on the Thirty Meter Telescope (TMT). APS is a Shack-Hartmann wave-front sensor that will be used to measure the alignment and phasing of the primary mirror segments, and the alignment of the secondary and tertiary mirrors. The APS system model contains the ow-down of the Level 1 TMT requirements to APS (Level 2) requirements, and from there to the APS sub-systems (Level 3) requirements. The model also contains the operating modes and scenarios for various activities, such as maintenance alignment, post-segment exchange alignment, and calibration activities. The requirements ow-down is captured in SysML requirements diagrams, and we describe the process of maintaining the DOORS database as the single-source-of-truth for requirements, while using the SysML model to capture the logic and notes associated with the ow-down. We also use the system model to capture any needed communications from APS to other TMT systems, and between the APS sub-systems. The operations are modeled using SysML activity diagrams, and will be used to specify the APS interface documents. The modeling tool can simulate the top level activities to produce sequence diagrams, which contain all the communications between the system and subsystem needed for that activity. By adding time estimates for the lowest level APS activities, a robust estimate for the total time on-sky that APS requires to align and phase the telescope can be obtained. This estimate will be used to verify that the time APS requires on-sky meets the Level 1 TMT requirements.

30 CFR 701.5 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., animal stocking requirements, scheduling of activities and design of sedimentation ponds in accordance... practice giving rise to the peril, would avoid exposure to the danger during the time necessary for... establishments. (g) Recreation. Land used for public or private leisure-time activities, including developed...

30 CFR 701.5 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., animal stocking requirements, scheduling of activities and design of sedimentation ponds in accordance... practice giving rise to the peril, would avoid exposure to the danger during the time necessary for... establishments. (g) Recreation. Land used for public or private leisure-time activities, including developed...

30 CFR 701.5 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., animal stocking requirements, scheduling of activities and design of sedimentation ponds in accordance... practice giving rise to the peril, would avoid exposure to the danger during the time necessary for... establishments. (g) Recreation. Land used for public or private leisure-time activities, including developed...

30 CFR 701.5 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., animal stocking requirements, scheduling of activities and design of sedimentation ponds in accordance... practice giving rise to the peril, would avoid exposure to the danger during the time necessary for... establishments. (g) Recreation. Land used for public or private leisure-time activities, including developed...

30 CFR 701.5 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., animal stocking requirements, scheduling of activities and design of sedimentation ponds in accordance... practice giving rise to the peril, would avoid exposure to the danger during the time necessary for... establishments. (g) Recreation. Land used for public or private leisure-time activities, including developed...

Meeting the Challenges of Exploration Systems: Health Management Technologies for Aerospace Systems With Emphasis on Propulsion

NASA Technical Reports Server (NTRS)

Melcher, Kevin J.; Sowers, T. Shane; Maul, William A.

2005-01-01

The constraints of future Exploration Missions will require unique Integrated System Health Management (ISHM) capabilities throughout the mission. An ambitious launch schedule, human-rating requirements, long quiescent periods, limited human access for repair or replacement, and long communication delays all require an ISHM system that can span distinct yet interdependent vehicle subsystems, anticipate failure states, provide autonomous remediation, and support the Exploration Mission from beginning to end. NASA Glenn Research Center has developed and applied health management system technologies to aerospace propulsion systems for almost two decades. Lessons learned from past activities help define the approach to proper ISHM development: sensor selection- identifies sensor sets required for accurate health assessment; data qualification and validation-ensures the integrity of measurement data from sensor to data system; fault detection and isolation-uses measurements in a component/subsystem context to detect faults and identify their point of origin; information fusion and diagnostic decision criteria-aligns data from similar and disparate sources in time and use that data to perform higher-level system diagnosis; and verification and validation-uses data, real or simulated, to provide variable exposure to the diagnostic system for faults that may only manifest themselves in actual implementation, as well as faults that are detectable via hardware testing. This presentation describes a framework for developing health management systems and highlights the health management research activities performed by the Controls and Dynamics Branch at the NASA Glenn Research Center. It illustrates how those activities contribute to the development of solutions for Integrated System Health Management.

The Ability To Activate and Inhibit Speeded Responses: Separate Developmental Trends.

ERIC Educational Resources Information Center

Band, Guido P. H.; van der Molen, Maurits W.; Overtoom, Carin C. E.; Verbaten, Marinus N.

2000-01-01

Compared 5-, 8-, and 11-year-olds and young adults on 6 speeded performance tasks, 4 requiring an inhibition of response activation. Analyzed reaction and inhibition times; found support for hypothesis of generalized developmental changes in response activation, but revealed less pronounced development of inhibition. Concluded that a nonselective…

14 CFR 1216.305 - Criteria for actions requiring environmental assessments.

Code of Federal Regulations, 2012 CFR

2012-01-01

... assessment are: (1) Specific spacecraft development and flight projects in space science. (2) Specific spacecraft development and flight projects in space and terrestrial applications. (3) Specific experimental... spacecraft development and flight projects. (2) R&D activities in space and terrestrial applications (e.g...

Active-learning assignments to integrate basic science and clinical course material.

PubMed

Marshall, Leisa L; Nykamp, Diane

2010-09-10

To develop, implement, and evaluate active-learning exercises requiring the integration and application of pathophysiology, medicinal chemistry, pharmacology, and therapeutics knowledge of osteoarthritis and rheumatoid arthritis to formulate therapeutic recommendations for patients with musculoskeletal disorders. Two team-based case study exercises, one evaluating a patient with osteoarthritis and the second, a patient with rheumatoid arthritis, were developed, incorporating material and questions from pathophysiology, medicinal chemistry, pharmacology, and therapeutics. The learning assignments were implemented in a required pharmacotherapy module. Student learning was evaluated using performance on the team-based case study exercises and on 2 examinations. A standard student course evaluation was used to assess students' impressions of the learning activity. The mean student grades for the osteoarthritis and rheumatoid arthritis activities were 9.1 and 8.9, respectively, on a 10-point scale. The majority of students indicated that the learning exercises were more than adequate to excellent in helping students learn. The addition of active-learning activities was successful in teaching pharmacy students the knowledge needed to formulate therapeutic recommendations for patients with musculoskeletal disorders.

Parental Influence on Psychological Value Perception of Co-Curricular Activities: Its Links with Improving Personality Traits of Higher Secondary Students

ERIC Educational Resources Information Center

Kumar, G. N. Sunith; Arockiasamy, S.

2012-01-01

Co-curricular activities provide prospects for better youth development and growth experiences. These activities are particularly good at providing opportunities for students to work in teams, to exercise leadership, and to take the initiative themselves. The active participation of the students is required to reap out maximum benefits out of such…

24 CFR 1000.106 - What families receiving assistance under title II of NAHASDA require HUD approval?

Code of Federal Regulations, 2010 CFR

2010-04-01

... under title II of NAHASDA require HUD approval? 1000.106 Section 1000.106 Housing and Urban Development... Housing Activities § 1000.106 What families receiving assistance under title II of NAHASDA require HUD... in §§ 1000.108 and 1000.110. (b) Assistance under section 201(b)(3) of NAHASDA for non-Indian...

Eye TVR: Eye Trauma and Visual Restoration Team

DTIC Science & Technology

2012-03-01

overall goal of this project is to develop a technology for non-invasive neuromodulation of retinal activity. Our approach is to measure the neuronal...technologies, including the millimeter wave source and the flexible multielectrode array, have been developed for non-invasive neuromodulation of retinal...activity. Further work is required to validate the feasibility of the proposed neuromodulation approach. (3) The strategy of joining a multisite

The Activities of the European Consortium on Nuclear Data Development and Analysis for Fusion

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fischer, U., E-mail: ulrich.fischer@kit.edu; Avrigeanu, M.; Avrigeanu, V.

This paper presents an overview of the activities of the European Consortium on Nuclear Data Development and Analysis for Fusion. The Consortium combines available European expertise to provide services for the generation, maintenance, and validation of nuclear data evaluations and data files relevant for ITER, IFMIF and DEMO, as well as codes and software tools required for related nuclear calculations.

22 CFR 1508.995 - Principal.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Relations AFRICAN DEVELOPMENT FOUNDATION GOVERNMENTWIDE DEBARMENT AND SUSPENSION (NONPROCUREMENT... influence or control the use of those funds; or, (3) Occupies a technical or professional position capable of substantially influencing the development or outcome of an activity required to perform the...

DigiSeis—A software component for digitizing seismic signals using the PC sound card

NASA Astrophysics Data System (ADS)

Amin Khan, Khalid; Akhter, Gulraiz; Ahmad, Zulfiqar

2012-06-01

An innovative software-based approach to develop an inexpensive experimental seismic recorder is presented. This approach requires no hardware as the built-in PC sound card is used for digitization of seismic signals. DigiSeis, an ActiveX component is developed to capture the digitized seismic signals from the sound card and deliver them to applications for processing and display. A seismic recorder application software SeisWave is developed over this component, which provides real-time monitoring and display of seismic events picked by a pair of external geophones. This recorder can be used as an educational aid for conducting seismic experiments. It can also be connected with suitable seismic sensors to record earthquakes. The software application and the ActiveX component are available for download. This component can be used to develop seismic recording applications according to user specific requirements.

Smart hands for the EVA retriever

NASA Technical Reports Server (NTRS)

Hess, Clifford W.; Li, Larry C.

1990-01-01

Dexterous, robotic hands are required for the extravehicular activity retriever (EVAR) system being developed by the NASA Johnson Space Center (JSC). These hands, as part of the EVAR system, must be able to grasp objects autonomously and securely which inadvertently separate from the Space Station. Development of the required hands was initiated in 1987. Outlined here are the hand development activities, including design considerations, progress to date, and future plans. Several types of dexterous hands that were evaluated, along with a proximity-sensing capability that was developed to initiate a reflexive, adaptive grasp, are described. The evaluations resulted in the design and fabrication of a 6-degree-of-freedom (DOF) hand that has two fingers and a thumb arranged in an anthropomorphic configuration. Finger joint force and position sensors are included in the design, as well as infrared proximity sensors which allow initiation of the grasp sequence when an object is detected within the grasp envelope.

Public Health and Climate Change Adaptation at the Federal Level: One Agency’s Response to Executive Order 13514

PubMed Central

Schramm, Paul J.; Luber, George

2014-01-01

Climate change will likely have adverse human health effects that require federal agency involvement in adaptation activities. In 2009, President Obama issued Executive Order 13514, Federal Leadership in Environmental, Energy, and Economic Performance. The order required federal agencies to develop and implement climate change adaptation plans. The Centers for Disease Control and Prevention (CDC), as part of a larger Department of Health and Human Services response to climate change, is developing such plans. We provide background on Executive Orders, outline tenets of climate change adaptation, discuss public health adaptation planning at both the Department of Health and Human Services and the CDC, and outline possible future CDC efforts. We also consider how these activities may be better integrated with other adaptation activities that manage emerging health threats posed by climate change. PMID:24432931

Public health and climate change adaptation at the federal level: one agency's response to Executive Order 13514.

PubMed

Hess, Jeremy J; Schramm, Paul J; Luber, George

2014-03-01

Climate change will likely have adverse human health effects that require federal agency involvement in adaptation activities. In 2009, President Obama issued Executive Order 13514, Federal Leadership in Environmental, Energy, and Economic Performance. The order required federal agencies to develop and implement climate change adaptation plans. The Centers for Disease Control and Prevention (CDC), as part of a larger Department of Health and Human Services response to climate change, is developing such plans. We provide background on Executive Orders, outline tenets of climate change adaptation, discuss public health adaptation planning at both the Department of Health and Human Services and the CDC, and outline possible future CDC efforts. We also consider how these activities may be better integrated with other adaptation activities that manage emerging health threats posed by climate change.

Development and analysis of SCR requirements tables for system scenarios

NASA Technical Reports Server (NTRS)

Callahan, John R.; Morrison, Jeffery L.

1995-01-01

We describe the use of scenarios to develop and refine requirement tables for parts of the Earth Observing System Data and Information System (EOSDIS). The National Aeronautics and Space Administration (NASA) is developing EOSDIS as part of its Mission-To-Planet-Earth (MTPE) project to accept instrument/platform observation requests from end-user scientists, schedule and perform requested observations of the Earth from space, collect and process the observed data, and distribute data to scientists and archives. Current requirements for the system are managed with tools that allow developers to trace the relationships between requirements and other development artifacts, including other requirements. In addition, the user community (e.g., earth and atmospheric scientists), in conjunction with NASA, has generated scenarios describing the actions of EOSDIS subsystems in response to user requests and other system activities. As part of a research effort in verification and validation techniques, this paper describes our efforts to develop requirements tables from these scenarios for the EOSDIS Core System (ECS). The tables specify event-driven mode transitions based on techniques developed by the Naval Research Lab's (NRL) Software Cost Reduction (SCR) project. The SCR approach has proven effective in specifying requirements for large systems in an unambiguous, terse format that enhance identification of incomplete and inconsistent requirements. We describe development of SCR tables from user scenarios and identify the strengths and weaknesses of our approach in contrast to the requirements tracing approach. We also evaluate the capabilities of both approach to respond to the volatility of requirements in large, complex systems.

Glutamyl-gamma-boronate inhibitors of bacterial Glu-tRNA(Gln) amidotransferase.

PubMed

Decicco, C P; Nelson, D J; Luo, Y; Shen, L; Horiuchi, K Y; Amsler, K M; Foster, L A; Spitz, S M; Merrill, J J; Sizemore, C F; Rogers, K C; Copeland, R A; Harpel, M R

2001-09-17

Analogues of glutamyl-gamma-boronate (1) were synthesized as mechanism-based inhibitors of bacterial Glu-tRNA(Gln) amidotransferase (Glu-AdT) and were designed to engage a putative catalytic serine nucleophile required for the glutaminase activity of the enzyme. Although 1 provides potent enzyme inhibition, structure-activity studies revealed a narrow range of tolerated chemical changes that maintained activity. Nonetheless, growth inhibition of organisms that require Glu-AdT by the most potent enzyme inhibitors appears to validate mechanism-based inhibitor design of Glu-AdT as an approach to antimicrobial development.

32 CFR 700.405 - Delegated authority and responsibility.

Code of Federal Regulations, 2010 CFR

2010-07-01

... education training and equal opportunities for personal advancement, and maintaining the morale and motivation of Navy personnel and the prestige of a Navy career; (4) To plan and provide health care for... determine the requirements of naval forces and activities, to include requirements for research, development...

78 FR 78968 - Agency Information Collection Activities: Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-27

... of Strategic Operations and Regulatory Affairs, Division of Regulations Development, Attention... Disclosure and Review Reporting Requirements CMS-724 Medicare/Medicaid Psychiatric Hospital Survey Data Under... Reporting Requirements; Use: Section 1003 of the Affordable Care Act adds a new section 2794 of the PHS Act...

14 CFR 1216.305 - Criteria for actions requiring environmental assessments.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Criteria for actions requiring environmental... spacecraft development and flight projects in space and terrestrial applications. (3) Specific experimental... experimental projects. (4) R&D (or SFCDC) activities in space transportation systems engineering and scientific...

75 FR 68215 - Direct Final Rule Staying Numeric Limitation for the Construction and Development Point Source...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-05

... required to obtain NPDES permit coverage and performing the following activities: Construction of buildings, including building, developing and general contracting. Heavy and civil 237 engineering construction... Rule Staying Numeric Limitation for the Construction and Development Point Source Category AGENCY...

Multi-tiered Approach to Development of Increased Throughput Assay Models to Assess Endocrine-Disrupting Activity of Chemicals

EPA Science Inventory

Screening for endocrine-disrupting chemicals (EDCs) requires sensitive, scalable assays. Current high-throughput screening (HTPS) approaches for estrogenic and androgenic activity yield rapid results, but many are not sensitive to physiological hormone concentrations, suggesting ...

OBESITY-INDUCED HYPERTENSION: INTERACTION OF NEUROHUMORAL AND RENAL MECHANISMS

PubMed Central

Hall, John E.; do Carmo, Jussara M.; da Silva, Alexandre A.; Wang, Zhen; Hall, Michael E.

2015-01-01

Excess weight gain, especially when associated with increased visceral adiposity, is a major cause of hypertension, accounting for 65–75% of the risk for human primary (essential) hypertension. Increased renal tubular sodium reabsorption impairs pressure natriuresis and plays an important role in initiating obesity hypertension. The mediators of abnormal kidney function and increased blood pressure during development of obesity hypertension include 1) physical compression of the kidneys by fat in and around the kidneys, 2) activation of the renin-angiotensin-aldosterone system (RAAS), and 3) increased sympathetic nervous system (SNS) activity. Activation of the RAAS system is likely due, in part, to renal compression as well as SNS activation. However, obesity also causes mineralocorticoid receptor activation independent of aldosterone or angiotensin II. The mechanisms for SNS activation in obesity have not been fully elucidated but appear to require leptin and activation of the brain melanocortin system. With prolonged obesity and development of target organ injury, especially renal injury, obesity-associated hypertension becomes more difficult to control, often requiring multiple antihypertensive drugs and treatment of other risk factors, including dyslipidemia, insulin resistance and diabetes, and inflammation. Unless effective anti-obesity drugs are developed, the impact of obesity on hypertension and related cardiovascular, renal and metabolic disorders is likely to become even more important in the future as the prevalence of obesity continues to increase. PMID:25767285

Necrotrophic pathogens use the salicylic acid signaling pathway to promote disease development in tomato.

PubMed

Rahman, Taha Abd El; Oirdi, Mohamed El; Gonzalez-Lamothe, Rocio; Bouarab, Kamal

2012-12-01

Plants use different immune pathways to combat pathogens. The activation of the jasmonic acid (JA)-signaling pathway is required for resistance against necrotrophic pathogens; however, to combat biotrophic pathogens, the plants activate mainly the salicylic acid (SA)-signaling pathway. SA can antagonize JA signaling and vice versa. NPR1 (noninducible pathogenesis-related 1) is considered a master regulator of SA signaling. NPR1 interacts with TGA transcription factors, ultimately leading to the activation of SA-dependent responses. SA has been shown to promote disease development caused by the necrotrophic pathogen Botrytis cinerea through NPR1, by suppressing the expression of two JA-dependent defense genes, proteinase inhibitors I and II. We show here that the transcription factor TGA1.a contributes to disease development caused by B. cinerea in tomato by suppressing the expression of proteinase inhibitors I and II. Finally, we present evidence that the SA-signaling pathway contributes to disease development caused by another necrotrophic pathogen, Alternaria solani, in tomato. Disease development promoted by SA through NPR1 requires the TGA1.a transcription factor. These data highlight how necrotrophs manipulate the SAsignaling pathway to promote their disease in tomato.

Information transfer satellite concept study. Volume 2: Technical

NASA Technical Reports Server (NTRS)

Bergin, P.; Kincade, C.; Kurpiewski, D.; Leinhaupel, F.; Millican, F.; Onstad, R.

1971-01-01

The ITS concept study is preceded by two requirements studies whose primary objectives are to identify viable demands and to develop the functional requirements associated with these demands. In addition to continuing this basic activity the ITS concept study objectives are to: (1) develop tools and techniques for planning advanced information transfer satellite communications systems, and to (2) select viable systems for further analysis both in their near-term and in the far-term aspects.

Photosynthetic activity during olive (Olea europaea) leaf development correlates with plastid biogenesis and Rubisco levels.

PubMed

Maayan, Inbar; Shaya, Felix; Ratner, Kira; Mani, Yair; Lavee, Shimon; Avidan, Benjamin; Shahak, Yosepha; Ostersetzer-Biran, Oren

2008-11-01

Olive leaves are known to mature slowly, reaching their maximum photosynthetic activity only after full leaf expansion. Poor assimilation rates, typical to young olive leaves, were previously associated with low stomata conductance. Yet, very little is known about chloroplast biogenesis throughout olive leaf development. Here, the photosynthetic activity and plastids development throughout leaf maturation is characterized by biochemical and ultrastructural analyses. Although demonstrated only low photosynthetic activity, the plastids found in young leaves accumulated both photosynthetic pigments and proteins required for photophosphorylation and carbon fixation. However, Rubisco (ribulose-1,5-bisphosphate carboxylase-oxygenase), which catalyzes the first major step of carbon fixation and one of the most abundant proteins in plants, could not be detected in the young leaves and only slowly accumulated throughout development. In fact, Rubisco levels seemed tightly correlated with the observed photosynthetic activities. Unlike Rubisco, numerous proteins accumulated in the young olive leaves. These included the early light induced proteins, which may be required to reduce the risk of photodamage, because of light absorption by photosynthetic pigments. Also, high levels of ribosomal L11 subunit, transcription factor elF-5A, Histones H2B and H4 were observed in the apical leaves, and in particular a plastidic-like aldolase, which accounted for approximately 30% of the total proteins. These proteins may upregulate in their levels to accommodate the high demand for metabolic energy in the young developing plant tissue, further demonstrating the complex sink-to-source relationship between young and photosynthetically active mature leaves.

Design and integrated operation of an innovative thermodynamic vent system concept

NASA Astrophysics Data System (ADS)

Fazah, Michel M.; Lak, Tibor; Nguyen, Han; Wood, Charles C.

1993-06-01

A unique zero-g thermodynamic vent system (TVS) is being developed by NASA's Marshall Space Flight Center (MSFC) and Rockwell International to meet cryogenic propellant management requirements for future space missions. The design is highly innovative in that it integrates the functions of a spray-bar tank mixer and a TVS. This concept not only satisfies the requirement for efficient tank mixing and zero-g venting but also accommodates thermal conditioning requirements for other components (e.g., engine feed lines, turbopumps, and liquid acquisition devices). In addition, operations can be extended to accomplish tank chill-down, no-vent fill, and emergency venting during zero-g propellant transfer. This paper describes the system performance characterization and future test activities that are part of MSFC's Multipurpose Hydrogen Test Bed (MHTB) program. The testing will demonstrate the feasibility and merit of the design, and serve as a proof-of-concept development activity.

A time-driven, activity-based costing methodology for determining the costs of red blood cell transfusion in patients with beta thalassaemia major.

PubMed

Burns, K E; Haysom, H E; Higgins, A M; Waters, N; Tahiri, R; Rushford, K; Dunstan, T; Saxby, K; Kaplan, Z; Chunilal, S; McQuilten, Z K; Wood, E M

2018-04-10

To describe the methodology to estimate the total cost of administration of a single unit of red blood cells (RBC) in adults with beta thalassaemia major in an Australian specialist haemoglobinopathy centre. Beta thalassaemia major is a genetic disorder of haemoglobin associated with multiple end-organ complications and typically requiring lifelong RBC transfusion therapy. New therapeutic agents are becoming available based on advances in understanding of the disorder and its consequences. Assessment of the true total cost of transfusion, incorporating both product and activity costs, is required in order to evaluate the benefits and costs of these new therapies. We describe the bottom-up, time-driven, activity-based costing methodology used to develop process maps to provide a step-by-step outline of the entire transfusion pathway. Detailed flowcharts for each process are described. Direct observations and timing of the process maps document all activities, resources, staff, equipment and consumables in detail. The analysis will include costs associated with performing these processes, including resources and consumables. Sensitivity analyses will be performed to determine the impact of different staffing levels, timings and probabilities associated with performing different tasks. Thirty-one process maps have been developed, with over 600 individual activities requiring multiple timings. These will be used for future detailed cost analyses. Detailed process maps using bottom-up, time-driven, activity-based costing for determining the cost of RBC transfusion in thalassaemia major have been developed. These could be adapted for wider use to understand and compare the costs and complexities of transfusion in other settings. © 2018 British Blood Transfusion Society.

Health-care district management information system plan: Review of operations analysis activities during calendar year 1975 and plan for continued research and analysis activities

NASA Technical Reports Server (NTRS)

Nielson, G. J.; Stevenson, W. G.

1976-01-01

Operations research activities developed to identify the information required to manage both the efficiency and effectiveness of the Veterans Administration (VA) health services as these services relate to individual patient care are reported. The clinical concerns and management functions that determine this information requirement are discussed conceptually. Investigations of existing VA data for useful management information are recorded, and a diagnostic index is provided. The age-specific characteristics of diseases and lengths of stay are explored, and recommendations for future analysis activities are articulated. The effect of the introduction of new technology to health care is also discussed.

Overview of Fluid Dynamics Activities at the Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

Garcia, Roberto; Griffin, Lisa W.; Wang, Ten-See

1999-01-01

Since its inception 40 years ago, Marshall Space Flight Center (MSFC) has had the need to maintain and advance state-of-the-art flow analysis and cold-flow testing capability to support its roles and missions. This overview discusses the recent organizational changes that have occurred at MSFC with emphasis on the resulting three groups that form the core of fluid dynamics expertise at MSFC: the Fluid Physics and Dynamics Group, the Applied Fluid Dynamics Analysis Group, and the Experimental Fluid Dynamics Group. Recently completed activities discussed include the analysis and flow testing in support of the Fastrac engine design, the X-33 vehicle design, and the X34 propulsion system design. Ongoing activities include support of the RLV vehicle design, Liquid Fly Back Booster aerodynamic configuration definition, and RLV focused technologies development. Other ongoing activities discussed are efforts sponsored by the Center Director's Discretionary Fund (CDDF) to develop an advanced incompressible flow code and to develop optimization techniques. Recently initiated programs and their anticipated required fluid dynamics support are discussed. Based on recent experiences and on the anticipated program needs, required analytical and experimental technique improvements are presented. Due to anticipated budgetary constraints, there is a strong need to leverage activities and to pursue teaming arrangements in order to advance the state-of-the-art and to adequately support concept development. Throughout this overview there is discussion of the lessons learned and of the capabilities demonstrated and established in support of the hardware development programs.

42 CFR 68c.2 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Secretary, wherein the applicant agrees to participate in research on infertility or contraceptive... as required. Contraceptive development means research whose ultimate goal is to provide new or... determined by the Secretary. Research on infertility or contraceptive development means activities which...

42 CFR 68c.2 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Secretary, wherein the applicant agrees to participate in research on infertility or contraceptive... as required. Contraceptive development means research whose ultimate goal is to provide new or... determined by the Secretary. Research on infertility or contraceptive development means activities which...

23 CFR 420.201 - What is the purpose of this subpart?

Code of Federal Regulations, 2010 CFR

2010-04-01

....201 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PLANNING AND RESEARCH PLANNING AND RESEARCH PROGRAM ADMINISTRATION Research, Development and Technology Transfer Program... requirements for research, development, and technology transfer (RD&T) activities, programs, and studies...

Implementation of a configurable laboratory information management system for use in cellular process development and manufacturing.

PubMed

Russom, Diana; Ahmed, Amira; Gonzalez, Nancy; Alvarnas, Joseph; DiGiusto, David

2012-01-01

Regulatory requirements for the manufacturing of cell products for clinical investigation require a significant level of record-keeping, starting early in process development and continuing through to the execution and requisite follow-up of patients on clinical trials. Central to record-keeping is the management of documentation related to patients, raw materials, processes, assays and facilities. To support these requirements, we evaluated several laboratory information management systems (LIMS), including their cost, flexibility, regulatory compliance, ongoing programming requirements and ability to integrate with laboratory equipment. After selecting a system, we performed a pilot study to develop a user-configurable LIMS for our laboratory in support of our pre-clinical and clinical cell-production activities. We report here on the design and utilization of this system to manage accrual with a healthy blood-donor protocol, as well as manufacturing operations for the production of a master cell bank and several patient-specific stem cell products. The system was used successfully to manage blood donor eligibility, recruiting, appointments, billing and serology, and to provide annual accrual reports. Quality management reporting features of the system were used to capture, report and investigate process and equipment deviations that occurred during the production of a master cell bank and patient products. Overall the system has served to support the compliance requirements of process development and phase I/II clinical trial activities for our laboratory and can be easily modified to meet the needs of similar laboratories.

Lobbying for international health: the link between good ideas and funded programs: Bread for the World and the Agency for International Development.

PubMed

Quinley, J C; Baker, T D

1986-07-01

Historically, the Agency for International Development (AIDS) health budget has been closely tied to overall development spending. A large increase in the international health appropriations in 1984 broke this pattern. Investigation shows that active grass roots organizing and congressional lobbying are the most likely responsible factors in the increase. Maintenance and expansion of this success will require increased recognition of and participation in these activities by individuals and organizations involved in international health.

Development of a portable teleoperated robot for the manipulation of a backhoe shovel for the restoration of disaster-stricken sites

NASA Astrophysics Data System (ADS)

Tanaka, Toshihiko; Sakai, Yosiharu; Konomi, Yosiyuki; Chayama, Kazuhiro; Minamoto, Masahiko; Matsunaga, Katsuya

1999-11-01

Restoration activities after disasters such as landslides or rock avalanches require rapid action, but in fact, in most cases these activities are very inefficient because of the danger of secondary disasters. A system which can operate reconstruction machinery by remote control was therefore developed, and it was installed on general-purpose construction machines (backhoe shovels). Control performance experiments and field experiments on this developed system were carried out, and its effectiveness was confirmed.

Leveraging the Continuum: A Novel Approach to Meeting Quality Improvement and Patient Safety Competency Requirements Across a Large Department of Medicine.

PubMed

Myers, Jennifer S; Bellini, Lisa M

2018-05-22

Quality improvement (QI) and patient safety (PS) are now core competencies across the medical education continuum. A major challenge to developing and implementing these new curricular requirements is the lack of faculty expertise. In 2015, the authors developed a centralized, vertically integrated, competency-based approach to meet the educational requirements in QI/PS across the continuum of graduate medical education in the Department of Medicine, Perelman School of Medicine, University of Pennsylvania. By leveraging the QI/PS expertise of one individual, the authors identified and trained core QI/PS faculty members and sequentially deployed QI/PS activities that were tailored to the learner level and specialty. The curriculum includes PS event reporting, systems thinking and root cause analysis skills, adverse event disclosure, and a QI workshop series and project. PS event reporting, an indication of engagement in PS culture, increased by 186% among interns, 384% among PGY 2 and 3 residents, and 613% among fellows between AYs 2013-2014 and 2016-2017. In AY 2017-2018, 9 faculty members and 40 fellows from 9 fellowships participated in the QI workshop series, and 53 fellows from 7 fellowships participated in the adverse event disclosure simulation activity. All educational activities were rated highly. The authors are expanding the adverse event disclosure activity to include residents and the remaining fellowship programs, identifying fellowships to pilot curricular efforts related to clinical quality metrics, developing introductory activities in basic QI/PS concepts for medical students, and evaluating the impact of efforts on the participating faculty members.

IPG Job Manager v2.0 Design Documentation

NASA Technical Reports Server (NTRS)

Hu, Chaumin

2003-01-01

This viewgraph presentation provides a high-level design of the IPG Job Manager, and satisfies its Master Requirement Specification v2.0 Revision 1.0, 01/29/2003. The presentation includes a Software Architecture/Functional Overview with the following: Job Model; Job Manager Client/Server Architecture; Job Manager Client (Job Manager Client Class Diagram and Job Manager Client Activity Diagram); Job Manager Server (Job Manager Client Class Diagram and Job Manager Client Activity Diagram); Development Environment; Project Plan; Requirement Traceability.

Incorporating active learning in psychiatry education.

PubMed

Kumar, Sonia; McLean, Loyola; Nash, Louise; Trigwell, Keith

2017-06-01

We aim to summarise the active learning literature in higher education and consider its relevance for postgraduate psychiatry trainees, to inform the development of a new Formal Education Course (FEC): the Master of Medicine (Psychiatry) at the University of Sydney. We undertook a literature search on 'active learning', 'flipped classroom', 'problem-based learning' and 'psychiatry education'. The effectiveness of active learning pedagogy in higher education is well supported by evidence; however, there have been few psychiatry-specific studies. A new 'flipped classroom' format was developed for the Master of Medicine (Psychiatry). Postgraduate psychiatry training is an active learning environment; the pedagogical approach to FECs requires further evaluation.

Feedback repression is required for mammalian circadian clock function.

PubMed

Sato, Trey K; Yamada, Rikuhiro G; Ukai, Hideki; Baggs, Julie E; Miraglia, Loren J; Kobayashi, Tetsuya J; Welsh, David K; Kay, Steve A; Ueda, Hiroki R; Hogenesch, John B

2006-03-01

Direct evidence for the requirement of transcriptional feedback repression in circadian clock function has been elusive. Here, we developed a molecular genetic screen in mammalian cells to identify mutants of the circadian transcriptional activators CLOCK and BMAL1, which were uncoupled from CRYPTOCHROME (CRY)-mediated transcriptional repression. Notably, mutations in the PER-ARNT-SIM domain of CLOCK and the C terminus of BMAL1 resulted in synergistic insensitivity through reduced physical interactions with CRY. Coexpression of these mutant proteins in cultured fibroblasts caused arrhythmic phenotypes in population and single-cell assays. These data demonstrate that CRY-mediated repression of the CLOCK/BMAL1 complex activity is required for maintenance of circadian rhythmicity and provide formal proof that transcriptional feedback is required for mammalian clock function.

45 CFR 98.53 - Matching fund requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION CHILD CARE AND DEVELOPMENT... funds for child care activities in the State that is at least equal to the State's share of expenditures...-effort requirement only if the State has not reduced its expenditures for full-day/full-year child care...

45 CFR 98.53 - Matching fund requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION CHILD CARE AND DEVELOPMENT... funds for child care activities in the State that is at least equal to the State's share of expenditures...-effort requirement only if the State has not reduced its expenditures for full-day/full-year child care...

45 CFR 98.53 - Matching fund requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Public Welfare Department of Health and Human Services GENERAL ADMINISTRATION CHILD CARE AND DEVELOPMENT... funds for child care activities in the State that is at least equal to the State's share of expenditures...-effort requirement only if the State has not reduced its expenditures for full-day/full-year child care...

45 CFR 98.53 - Matching fund requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION CHILD CARE AND DEVELOPMENT... funds for child care activities in the State that is at least equal to the State's share of expenditures...-effort requirement only if the State has not reduced its expenditures for full-day/full-year child care...

45 CFR 98.53 - Matching fund requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION CHILD CARE AND DEVELOPMENT... funds for child care activities in the State that is at least equal to the State's share of expenditures...-effort requirement only if the State has not reduced its expenditures for full-day/full-year child care...

40 CFR 123.63 - Criteria for withdrawal of State programs.

Code of Federal Regulations, 2014 CFR

2014-07-01

... programs. 123.63 Section 123.63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER... requirements of this part, including: (i) Failure to exercise control over activities required to be regulated... regulatory program for developing water quality-based effluent limits in NPDES permits. (6) Where a Great...

40 CFR 123.63 - Criteria for withdrawal of State programs.

Code of Federal Regulations, 2012 CFR

2012-07-01

... programs. 123.63 Section 123.63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER... requirements of this part, including: (i) Failure to exercise control over activities required to be regulated... regulatory program for developing water quality-based effluent limits in NPDES permits. (6) Where a Great...

Inquiry-Based Instruction of Compound Microscopy Using Simulated Paleobiogeography

ERIC Educational Resources Information Center

Hodgson, Jay Y. S.; Mateer, Scott C.

2015-01-01

The compound microscope is an important tool in biology, and mastering it requires repetition. Unfortunately, introductory activities for students can be formulaic, and consequently, students are often unengaged and fail to develop the required experience to become proficient in microscopy. To engage students, increase repetition, and develop…

75 FR 51027 - Commission Information Collection Activities (FERC-917 and FERC-918) 1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-18

... public utilities, working through the North American Electric Reliability Corporation (NERC), to develop... required a NERC/NAESB \\3\\ team to draft and review Order No. 890 reliability standards and business... revise reliability standards to require the exchange of data and coordination among transmission...

Development of Students' Critical-Reasoning Skills through Content-Focused Activities in a General Education Course

ERIC Educational Resources Information Center

Fencl, Heidi S.

2010-01-01

Students in a general education science course made significant gains in scientific reasoning skills when they were taught using carefully designed hands-on activities and writing assignments. The activities required students to make use of scientific skills such as graphing, predicting outcomes under changing conditions, or designing experiments,…

Development of flight experiment task requirements. Volume 2: Technical Report. Part 1: Program report and Appendices A-G

NASA Technical Reports Server (NTRS)

Hatterick, G. R.

1972-01-01

Activities are documented of the study to determine skills required of on-orbit crew personnel of the space shuttle. The material is presented in four sections that include: (1) methodology for identifying flight experiment task-skill requirements, (2) task-skill analysis of selected flight experiments, (3) study results and conclusions, and (4) new technology.

77 FR 37374 - Nominations for Members of the National Organic Standards Board

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-21

... is required to establish an NOSB. The purpose of the NOSB is to assist in the development of a... practical experience in the organic community; demonstrated experience in the development of public policy... organizations; participation in standards development or involvement in educational outreach activities; a...

Computer Needs and Computer Problems in Developing Countries.

ERIC Educational Resources Information Center

Huskey, Harry D.

A survey of the computer environment in a developing country is provided. Levels of development are considered and the educational requirements of countries at various levels are discussed. Computer activities in India, Burma, Pakistan, Brazil and a United Nations sponsored educational center in Hungary are all described. (SK/Author)

Executive Development: Meeting the Needs of Top Teams and Boards.

ERIC Educational Resources Information Center

Jackson, Sheila; Farndale, Elaine; Kakabadse, Andrew

2003-01-01

A literature review and six case studies examined the roles and responsibilities of the chief executive officers and board chairs, the capabilities required for success, and related executive development activity. Findings include the importance of focusing executive development on capability enhancement to ensure that it supports organizational…

Expanding the Scope of Faculty Educator Development for Health Care Professionals

ERIC Educational Resources Information Center

Lewis, Kadriye O.; Baker, Raymond C.

2009-01-01

Although many medical institutions offer faculty development in education, this does not provide the in-depth knowledge of the science of teaching required for medical education research and careers in medical education. This paper describes our expanding faculty development activities at Cincinnati Children's Hospital Medical Center (CCHMC) that…

A Pharmacy Course on Leadership and Leading Change

PubMed Central

Traynor, Andrew P.; Janke, Kristin K.

2009-01-01

Objective To develop and implement a course that develops pharmacy students' leadership skills and encourages them to become leaders within the profession. Design A leadership course series was offered to pharmacy students on 2 campuses. The series incorporated didactic, experiential, and self-directed learning activities, and focused on developing core leadership skills, self-awareness, and awareness of the process for leading change. Assessment Students reported increased knowledge and confidence in their ability to initiate and lead efforts for change. The learning activities students' valued most were the StrengthsFinder assessment (67% of students rated “very useful”) and a Leadership Networking Partners (LNP) program (83% of students rated “very useful”). Conclusion Teaching leadership skills poses a significant challenge in curriculum development and requires multifaceted course design elements that resonate with students and engage the practice community. Addressing these requirements results in a high level of student engagement and a desire to continue the development of leadership skills. PMID:19513161

Aquatics for the Handicapped--A Review of Literature.

ERIC Educational Resources Information Center

Christie, Irene

1985-01-01

The author reviews the literature on aquatic activity for the disabled, discussing the physical, physiological, psychological, and sociological benefits of swimming and water safety activities. Unique properties of water and legal requirements regarding physical education of the handicapped, specifically citing the development of skills in…

AN INTEGRATIVE WATERSHED MODELING FRAMEWORK (IWMF) FOR ECOSYSTEM SUSTAINABILITY ASSESSMENT AT THE WATERSHED SCALE

EPA Science Inventory

The public's right to continued access and use of ecosystem services requires an evaluation of the environmental risks that are associated with activities such as urban development, agriculture, forestry, mining, water withdrawal, and dam construction. Human activities can cause ...

Physical Education: Should It Be in the Core Curriculum?

ERIC Educational Resources Information Center

Gabbard, Carl

2000-01-01

Recent research suggests that today's children are less physically active and more overweight/obese than those of previous generations. A superior physical education program hires college-educated specialists, requires daily physical activities, stresses improvement-oriented fitness education and skill development, includes all children, and…

Teaching Strategies: The Family Fun Fair Connection.

ERIC Educational Resources Information Center

Harris, Paulette; Grubb, James

1997-01-01

Notes Augusta State University's "Family Fun Fair," developed in 1981 by early childhood teacher candidates, and now a major requirement of one of Augusta's early childhood courses. Describes several fun fair activities and how activities devised by the students are evaluated by the instructor. (HTH)

Development of Fundamental Technologies for Micro Bioreactors

NASA Astrophysics Data System (ADS)

Sato, Kiichi; Kitamori, Takehiko

This chapter reviews the development of fundamental technologies required for microchip-based bioreactors utilizing living mammalian cells and pressure driven flow. The most important factor in the bioreactor is the cell culture. For proper cell culturing, continuous medium supply from a microfluidic channel and appropriate modification of the channel surface to accommodate cell attachment is required. Moreover, the medium flow rate should be chosen carefully, because shear stress affects cell activity. The techniques presented here could be applied to the development of micro bioreactors such as microlivers, pigment production by plant cells, and artificial insemination.

HOMER® Micropower Optimization Model

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lilienthal, P.

2005-01-01

NREL has developed the HOMER micropower optimization model. The model can analyze all of the available small power technologies individually and in hybrid configurations to identify least-cost solutions to energy requirements. This capability is valuable to a diverse set of energy professionals and applications. NREL has actively supported its growing user base and developed training programs around the model. These activities are helping to grow the global market for solar technologies.

Atmospheric Data Requirements for Battlefield Obscuration Applications.

DTIC Science & Technology

1980-06-01

34 ECOM-5572, August 1975. 9. Miers, B., G. Blackman, D. Langer, and N. Lorimier, " Analysis of SMS/GOES Film Data," ECOM-5573, September 1975. 10...ATEC-PL-M Combined Arms Combat Developments Activity; ATZLCA-RS Concepts Analysis Agency; CSCA-SMC Dugway Proving Ground; STEDP-PP Electronic Warfare...CS-SA Materiel Systems Analysis Activity, Ground Warfare Division; DRXSY-GS Missile Research and Development Command, Research Directorate; DRDMI-TRA

SAP is required for the development of innate phenotype in H2-M3-restricted CD8+ T cells1

PubMed Central

Bediako, Yaw; Bian, Yao; Zhang, Hong; Cho, Hoonsik; Stein, Paul L.; Wang, Chyung-Ru

2012-01-01

H2-M3-restricted T cells have a pre-activated surface phenotype, rapidly expand and produce cytokines upon stimulation and as such, are classified as innate T cells. Unlike most innate T cells, M3-restricted T cells also express CD8αβ co-receptors and a diverse TCR repertoire: hallmarks of conventional MHC Ia-restricted CD8+ T cells. Although iNKT cells are also innate lymphocytes, they are selected exclusively on hematopoietic cells (HC), while M3-restricted T cells can be selected on either hematopoietic or thymic epithelial cells (TEC). Moreover, their phenotypes differ depending on what cells mediate their selection. Though there is a clear correlation between selection on HC and development of innate phenotype, the underlying mechanism remains unclear. SAP is required for the development of iNKT cells and mediates signals from SLAM receptors that are exclusively expressed on HC. Based on their dual selection pathway, M3-restricted T cells present a unique model for studying the development of innate T cell phenotype. Using both polyclonal and transgenic mouse models we demonstrate that while M3-restricted T cells are capable of developing in the absence of SAP, SAP is required for HC-mediated selection, development of pre-activated phenotype and heightened effector functions of M3-restricted T cells. These findings are significant because they directly demonstrate the need for SAP in HC-mediated acquisition of innate T cell phenotype and suggest that due to their SAP-dependent HC-mediated selection, M3-restricted T cells develop a pre-activated phenotype and an intrinsic ability to proliferate faster upon stimulation, allowing for an important role in the early response to infection. PMID:23041566

Active-learning strategies to develop health literacy knowledge and skills.

PubMed

Devraj, Radhika; Butler, Lakesha M; Gupchup, Gireesh V; Poirier, Therese I

2010-10-11

To implement active-learning exercises in a required pharmacy course and assess their impact on students' knowledge and confidence in identifying and communicating with patients with low health literacy, as part of a required course in cultural competency, health literacy, and health beliefs. Active-learning activities including administering health literacy assessments, identifying informal signs of low health literacy, conducting mock patient counseling sessions, rating the readability of drug information, analyzing information in drug advertisements, and writing patient education materials were incorporated into the 6-sesssion health literacy portion of the course. A pretest and posttest showed that students' knowledge of health literacy increased, and a retrospective pretest found improvement in students' confidence in their ability to care for patients with low health literacy. In-class discussions provided informal evidence that students gained new knowledge from the active-learning activities. The addition of active-learning activities was effective in teaching health literacy concepts to pharmacy students.

Transfer Learning for Activity Recognition: A Survey

PubMed Central

Cook, Diane; Feuz, Kyle D.; Krishnan, Narayanan C.

2013-01-01

Many intelligent systems that focus on the needs of a human require information about the activities being performed by the human. At the core of this capability is activity recognition, which is a challenging and well-researched problem. Activity recognition algorithms require substantial amounts of labeled training data yet need to perform well under very diverse circumstances. As a result, researchers have been designing methods to identify and utilize subtle connections between activity recognition datasets, or to perform transfer-based activity recognition. In this paper we survey the literature to highlight recent advances in transfer learning for activity recognition. We characterize existing approaches to transfer-based activity recognition by sensor modality, by differences between source and target environments, by data availability, and by type of information that is transferred. Finally, we present some grand challenges for the community to consider as this field is further developed. PMID:24039326

The integration of the risk management process with the lifecycle of medical device software.

PubMed

Pecoraro, F; Luzi, D

2014-01-01

The application of software in the Medical Device (MD) domain has become central to the improvement of diagnoses and treatments. The new European regulations that specifically address software as an important component of MD, require complex procedures to make software compliant with safety requirements, introducing thereby new challenges in the qualification and classification of MD software as well as in the performance of risk management activities. Under this perspective, the aim of this paper is to propose an integrated framework that combines the activities to be carried out by the manufacturer to develop safe software within the development lifecycle based on the regulatory requirements reported in US and European regulations as well as in the relevant standards and guidelines. A comparative analysis was carried out to identify the main issues related to the application of the current new regulations. In addition, standards and guidelines recently released to harmonise procedures for the validation of MD software have been used to define the risk management activities to be carried out by the manufacturer during the software development process. This paper highlights the main issues related to the qualification and classification of MD software, providing an analysis of the different regulations applied in Europe and the US. A model that integrates the risk management process within the software development lifecycle has been proposed too. It is based on regulatory requirements and considers software risk analysis as a central input to be managed by the manufacturer already at the initial stages of the software design, in order to prevent MD failures. Relevant changes in the process of MD development have been introduced with the recognition of software being an important component of MDs as stated in regulations and standards. This implies the performance of highly iterative processes that have to integrate the risk management in the framework of software development. It also makes it necessary to involve both medical and software engineering competences to safeguard patient and user safety.

Space Station Furnace Facility Preliminary Project Implementation Plan (PIP). Volume 2, Appendix 2

NASA Technical Reports Server (NTRS)

Perkey, John K.

1992-01-01

The Space Station Furnace Facility (SSFF) is an advanced facility for materials research in the microgravity environment of the Space Station Freedom and will consist of Core equipment and various sets of Furnace Module (FM) equipment in a three-rack configuration. This Project Implementation Plan (PIP) document was developed to satisfy the requirements of Data Requirement Number 4 for the SSFF study (Phase B). This PIP shall address the planning of the activities required to perform the detailed design and development of the SSFF for the Phase C/D portion of this contract.

EthA, a Common Activator of Thiocarbamide-Containing Drugs Acting on Different Mycobacterial Targets▿

PubMed Central

Dover, Lynn G.; Alahari, Anuradha; Gratraud, Paul; Gomes, Jessica M.; Bhowruth, Veemal; Reynolds, Robert C.; Besra, Gurdyal S.; Kremer, Laurent

2007-01-01

Many of the current antimycobacterial agents require some form of cellular activation unmasking reactive groups, which in turn will bind to their specific targets. Therefore, understanding the mechanisms of activation of current antimycobacterials not only helps to decipher mechanisms of drug resistance but may also facilitate the development of alternative activation strategies or of analogues that do not require such processes. Herein, through the use of genetically defined strains of Mycobacterium bovis BCG we provide evidence that EthA, previously shown to activate ethionamide, also converts isoxyl (ISO) and thiacetazone (TAC) into reactive species. These results were further supported by the development of an in vitro assay using purified recombinant EthA, which allowed direct assessment of the metabolism of ISO. Interestingly, biochemical analysis of [14C]acetate-labeled cultures suggested that all of these EthA-activated drugs inhibit mycolic acid biosynthesis via different mechanisms through binding to specific targets. This report is also the first description of the molecular mechanism of action of TAC, a thiosemicarbazone antimicrobial agent that is still used in the treatment of tuberculosis as a second-line drug in many developing countries. Altogether, the results suggest that EthA is a common activator of thiocarbamide-containing drugs. The broad specificity of EthA can now be used to improve the activation process of these drugs, which may help overcome the toxicity problems associated with clinical thiocarbamide use. PMID:17220416

Virtual environment and computer-aided technologies used for system prototyping and requirements development

NASA Technical Reports Server (NTRS)

Logan, Cory; Maida, James; Goldsby, Michael; Clark, Jim; Wu, Liew; Prenger, Henk

1993-01-01

The Space Station Freedom (SSF) Data Management System (DMS) consists of distributed hardware and software which monitor and control the many onboard systems. Virtual environment and off-the-shelf computer technologies can be used at critical points in project development to aid in objectives and requirements development. Geometric models (images) coupled with off-the-shelf hardware and software technologies were used in The Space Station Mockup and Trainer Facility (SSMTF) Crew Operational Assessment Project. Rapid prototyping is shown to be a valuable tool for operational procedure and system hardware and software requirements development. The project objectives, hardware and software technologies used, data gained, current activities, future development and training objectives shall be discussed. The importance of defining prototyping objectives and staying focused while maintaining schedules are discussed along with project pitfalls.

Clinical leadership development requires system-wide interventions, not just courses.

PubMed

Swanwick, Tim; McKimm, Judy

2012-04-01

This is the third article in a series on clinical leadership and medical education. In the first two articles in this series we looked at the nature of leadership and examined professional outcomes, standards and competency frameworks from around the world that describe what it is we are trying to instil in medical students and doctors in postgraduate training. In this article we explore current trends in leadership development and describe broad approaches to clinical leadership development, highlighting those strategies that are likely to be more (or less) successful. Narrative review and discussion. Key trends and principles for best practice in leadership development are identified. Recommendations for the design of leadership development programmes are made alongside suggestions for system-wide interventions. Leadership development should be both drawn from and embedded in work-based activities, and as far as possible linked to the development of the organisation as a whole. Intervening at the level of the individual may not be enough. System-wide interventions are required that actively engage students and trainees in the practices of management and leadership, and involve them early. © Blackwell Publishing Ltd 2012.

AO WFS detector developments at ESO to prepare for the E-ELT

NASA Astrophysics Data System (ADS)

Downing, Mark; Casali, Mark; Finger, Gert; Lewis, Steffan; Marchetti, Enrico; Mehrgan, Leander; Ramsay, Suzanne; Reyes, Javier

2016-07-01

ESO has a very active on-going AO WFS detector development program to not only meet the needs of the current crop of instruments for the VLT, but also has been actively involved in gathering requirements, planning, and developing detectors and controllers/cameras for the instruments in design and being proposed for the E-ELT. This paper provides an overall summary of the AO WFS Detector requirements of the E-ELT instruments currently in design and telescope focal units. This is followed by a description of the many interesting detector, controller, and camera developments underway at ESO to meet these needs; a) the rationale behind and plan to upgrade the 240x240 pixels, 2000fps, "zero noise", L3Vision CCD220 sensor based AONGC camera; b) status of the LGSD/NGSD High QE, 3e- RoN, fast 700fps, 1760x1680 pixels, Visible CMOS Imager and camera development; c) status of and development plans for the Selex SAPHIRA NIR eAPD and controller. Most of the instruments and detector/camera developments are described in more detail in other papers at this conference.

Protein/energy ratios of current diets in developed and developing countries compared with a safe protein/energy ratio: implications for recommended protein and amino acid intakes.

PubMed

Millward, D Joe; Jackson, Alan A

2004-05-01

Revised estimates of protein and amino acid requirements are under discussion by the Food and Agriculture Organization (FAO)/World Health Organizaion (WHO), and have been proposed in a recent report on Dietary Reference Intakes (DRIs) from the USA. The nature and magnitude of these requirements are not entirely resolved, and no consideration has been given to the potential influence of metabolic adaptation on dietary requirements. We have examined the implications of these new values, and of the conceptual metabolic framework in which they are used, for defining the nutritional adequacy of protein intakes in developed and developing countries. We have expressed proposed values for protein requirements in relation to energy requirements, predicted for physical activity levels of 1.5, 1.75 and 2.0 times basal metabolic rate, in order to generate reference ratios for protein energy/total energy (reference P/E ratio) as a function of age, body weight, gender and physical activity level. Proposed values for amino acid requirements have been used to adjust the available digestible P/E ratio of foods and diets for protein quality. Focusing on the diets of UK omnivores and vegetarians and on diets in India, the risk of protein deficiency is evaluated from a comparison of P/E ratios of metabolic requirements with protein-quality-adjusted P/E ratios of intakes. A qualitative and conservative estimate of risk of deficiency is made by comparing the adjusted P/E ratio of the intake with a reference P/E ratio calculated for age, body weight, gender and physical activity according to FAO/WHO/United Nations University. A semi-quantitative estimate of risk of deficiency has also been made by the cut point approach, calculated as the proportion of the intake distribution below the mean P/E ratio of the requirement. Values for the quality-adjusted P/E ratio of the diet range from 0.126 for the UK omnivore diet to 0.054 for a rice-based diet of adults in West Bengal, which is lysine-limited, falling to 0.050 for 1-year-old children. The reference P/E ratio for men and women increases with age, is higher for females than males, is higher for small compared with large adults at any age and decreases with physical activity. Thus if a particular diet is potentially limiting in protein, protein deficiency is most likely in large, elderly sedentary women followed by the adolescent female and least likely in moderately active young children, the opposite of what has usually been assumed. Within the currently accepted framework, the diets do not meet the protein needs of the entire population of the UK, have a significant risk of deficiency throughout India for all except extremely active small adults, and are grossly inadequate for all population groups, apart from physically active young children in West Bengal, regardless of body weight or level of food intake. The lysine limitation of the cereal-based Indian diets is dependent on the choice of lysine requirement values from the published range. We consider that the value selected is too high, because of uncertainties and inconsistencies in the approaches used. A more appropriate choice from the lower end of the range would remove the lysine limitation of cereal-based diets, and reduce some of the perceived risk of deficiency. However, diets remain limited by the amount of digestible protein for many population groups, especially in West Bengal. In the context of risk management, one option would be to accept the current values and the conceptual metabolic framework within which they have been derived. This would have major implications for the supplies of high-quality protein to the developing countries. An alternative option would be to re-evaluate the currently proposed values for the requirements for protein and amino acids. We conclude that the choice of values for the adult lysine requirement should be re-evaluated and that serious consideration should be given to the extent to which adaptive mechanisms might enable the metabolic requirement for protein to be met from current intakes. This will entail a better understanding of the relationships between dietary protein and health.

Active prospective control is required for effective sensorimotor learning.

PubMed

Snapp-Childs, Winona; Casserly, Elizabeth; Mon-Williams, Mark; Bingham, Geoffrey P

2013-01-01

Passive modeling of movements is often used in movement therapy to overcome disabilities caused by stroke or other disorders (e.g. Developmental Coordination Disorder or Cerebral Palsy). Either a therapist or, recently, a specially designed robot moves or guides the limb passively through the movement to be trained. In contrast, action theory has long suggested that effective skill acquisition requires movements to be actively generated. Is this true? In view of the former, we explicitly tested the latter. Previously, a method was developed that allows children with Developmental Coordination Disorder to produce effective movements actively, so as to improve manual performance to match that of typically developing children. In the current study, we tested practice using such active movements as compared to practice using passive movement. The passive movement employed, namely haptic tracking, provided a strong test of the comparison, one that showed that the mere inaction of the muscles is not the problem. Instead, lack of prospective control was. The result was no effective learning with passive movement while active practice with prospective control yielded significant improvements in performance.

Developing and Sustaining Recovery-Orientation in Mental Health Practice: Experiences of Occupational Therapists.

PubMed

Nugent, Alexandra; Hancock, Nicola; Honey, Anne

2017-01-01

Internationally, mental health policy requires clinicians to shift from a medical to a recovery-oriented approach. However, there is a significant lag in the translation of policy into practice. Occupational therapists have been identified as ideally situated to be recovery-oriented yet limited research exploring how they do this exists. This study aimed to explore Australian occupational therapists' experiences of developing and sustaining recovery-orientation in mental health practice. Semistructured, in-depth interviews were conducted with twelve occupational therapists working across different mental health service types. Participants identified themselves as being recovery-oriented. Data were analysed using constant comparative analysis. Occupational therapists described recovery-oriented practice as an active, ongoing, and intentional process of seeking out knowledge, finding fit between understandings of recovery-oriented practice and their professional identity, holding hope, and developing confidence through clinical reasoning. Human and systemic aspects of therapists' workplace environment influenced this process. Being a recovery-oriented occupational therapist requires more than merely accepting a specific framework. It requires commitment and ongoing work to develop and sustain recovery-orientation. Occupational therapists are called to extend current leadership activity beyond their workplace and to advocate for broader systemic change.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

The Office of Civilian Radioactive Waste Management Systems Engineering Management Plan (OCRWM SEMP) specifies the technical management approach for the development of the waste management system, and specifies the approach for the development of each of the system elements -- the waste acceptance system, the transportation system, the Monitored Retrievable Storage (MRS) facility, and the mined geologic disposal system, which includes site characterization activity. The SEMP also delineates how systems engineering will be used by OCRWM to describe the system development process; it identifies responsibilities for its implementation, and specifies the minimum requirements for systems engineering. It also identifies themore » close interrelationship of system engineering and licensing processes. This SEMP, which is a combined OCRWM and M&O SEMP, is part of the top-level program documentation and is prepared in accordance with the direction provided in the Program Management System Manual (PMSM). The relationship of this document to other top level documents in the CRWMS document hierarchy is defined in the PMSM. A systems engineering management plan for each project, which specifies the actions to be taken in implementing systems engineering at the project level, shall be prepared by the respective project managers. [``Program`` refers to the CRWMS-wide activity and ``project`` refers to that level responsible for accomplishing the specific activities of that segment of the program.] The requirements for the project level SEMPs are addressed in Section 4.2.2.2. They represent the minimum set of requirements, and do not preclude the broadening of systems engineering activities to meet the specific needs of each project.« less

Open Source Patient-Controlled Analgesic Pump Requirements Documentation

PubMed Central

Larson, Brian R.; Hatcliff, John; Chalin, Patrice

2014-01-01

The dynamic nature of the medical domain is driving a need for continuous innovation and improvement in techniques for developing and assuring medical devices. Unfortunately, research in academia and communication between academics, industrial engineers, and regulatory authorities is hampered by the lack of realistic non-proprietary development artifacts for medical devices. In this paper, we give an overview of a detailed requirements document for a Patient-Controlled Analgesic (PCA) pump developed under the US NSF’s Food and Drug Administration (FDA) Scholar-in-Residence (SIR) program. This 60+ page document follows the methodology outlined in the US Federal Aviation Administrations (FAA) Requirements Engineering Management Handbook (REMH) and includes a domain overview, use cases, statements of safety & security requirements, and formal top-level system architectural description. Based on previous experience with release of a requirements document for a cardiac pacemaker that spawned a number of research and pedagogical activities, we believe that the described PCA requirements document can be an important research enabler within the formal methods and software engineering communities. PMID:24931440

English Skills for Engineers Required by the English Technical Writing Test

NASA Astrophysics Data System (ADS)

Kyouno, Noboru

Japanese English education has focused mainly on teaching passive skills such as reading and listening, whereas actual business activities in society require active skills such as writing and speaking in addition to the passive skills. This educational situation is estimated to be a reason Japanese engineers are less confident in writing and speaking than in reading and listening. This paper focuses on details of the English Technical Writing Test provided by the Japan Society of Technical Communication and emphasizes the importance of the active skills, mainly focusing on what skills should be taught in the future and how to develop these skills. This paper also stresses the necessity of learning rhetoric-related skills, concept of information words, as well as paragraph reading and writing skills based on the concept of the 3Cs (Correct, Clear, and Concise) as a means to develop technical writing skills for engineers.

AdaNET research plan

NASA Technical Reports Server (NTRS)

Mcbride, John G.

1990-01-01

The mission of the AdaNET research effort is to determine how to increase the availability of reusable Ada components and associated software engineering technology to both private and Federal sectors. The effort is structured to define the requirements for transfer of Federally developed software technology, study feasible approaches to meeting the requirements, and to gain experience in applying various technologies and practices. The overall approach to the development of the AdaNET System Specification is presented. A work breakdown structure is presented with each research activity described in detail. The deliverables for each work area are summarized. The overall organization and responsibilities for each research area are described. The schedule and necessary resources are presented for each research activity. The estimated cost is summarized for each activity. The project plan is fully described in the Super Project Expert data file contained on the floppy disk attached to the back cover of this plan.

Differentiation of δ, μ, and κ opioid receptor agonists based on pharmacophore development and computed physicochemical properties

NASA Astrophysics Data System (ADS)

Filizola, Marta; Villar, Hugo O.; Loew, Gilda H.

2001-04-01

Compounds that bind with significant affinity to the opioid receptor types, δ, μ, and κ, with different combinations of activation and inhibition at these three receptors could be promising behaviorally selective agents. Working on this hypothesis, the chemical moieties common to three different sets of opioid receptor agonists with significant affinity for each of the three receptor types δ, μ, or κ were identified. Using a distance analysis approach, common geometric arrangements of these chemical moieties were found for selected δ, μ, or κ opioid agonists. The chemical and geometric commonalities among agonists at each opioid receptor type were then compared with a non-specific opioid recognition pharmacophore recently developed. The comparison provided identification of the additional requirements for activation of δ, μ, and κ opioid receptors. The distance analysis approach was able to clearly discriminate κ-agonists, while global molecular properties for all compounds were calculated to identify additional requirements for activation of δ and μ receptors. Comparisons of the combined geometric and physicochemical properties calculated for each of the three sets of agonists allowed the determination of unique requirements for activation of each of the three opioid receptors. These results can be used to improve the activation selectivity of known opioid agonists and as a guide for the identification of novel selective opioid ligands with potential therapeutic usefulness.

Understanding continuous professional development participation and choice of mid-career general dental practitioners.

PubMed

Brown, T; Wassif, H S

2017-02-01

Participating in continuing professional development (CPD) activities is a requirement for dental practitioners to keep their skills and knowledge up to date. Understanding the ways dental practitioners engage with professional development and the impact on practice is not fully known (Eaton et al. 2011, http://www.gdc-uk.org/Aboutus/policy/Documents/Impact%20Of%20CPD%20In%20Dentistry.pdf). The aim of this study was to gain insights into the ways that dentists reflect on their professional development and what may be influencing their choices. Empirical qualitative data were collected by semi-structured interviewing of five mid-career dentists. Using grounded theory, the data were analysed for themes about CPD choice and participation. Three themes were identified as influences to dentists' choices of CPD with pragmatic considerations of how new learning could benefit their patients and their practices. Dental practitioners were influenced by the requirements of external regulatory bodies which they did not consider to necessarily improve practice. Dentists working in primary care in the UK are undertaking CPD which is influenced by the pragmatic requirements of running a small business and to meet regulatory requirements. In this sample, dentists are not critically reflecting on their education needs when choosing their CPD activity. Protected learning time and organisational feedback and support are recommended as a way to promote more meaningful reflection on learning and to improve professional development. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Information requirements and methodology for development of an EVA crewmember's heads up display

NASA Astrophysics Data System (ADS)

Petrek, J. S.

This paper presents a systematic approach for developing a Heads Up Display (HUD) to be used within the helmet of the Extra Vehicular Activity (EVA) crewmember. The information displayed on the EVA HUD will be analogous to EVA Flight Data File (FDF) information, which is an integral part of NASA's current Space Transportation System. Another objective is to determine information requirements and media techniques ultimately leading to the helmet-mounted HUD presentation technique.

Distinct regions of the interleukin-7 receptor regulate different Bcl2 family members.

PubMed

Jiang, Qiong; Li, Wen Qing; Hofmeister, Robert R; Young, Howard A; Hodge, David R; Keller, Jonathan R; Khaled, Annette R; Durum, Scott K

2004-07-01

The antiapoptotic function of the interleukin-7 (IL-7) receptor is related to regulation of three members of the Bcl2 family: synthesis of Bcl2, phosphorylation of Bad, and cytosolic retention of Bax. Here we show that, in an IL-7-dependent murine T-cell line, different regions of the IL-7 receptor initiate the signal transduction pathways that regulate these proteins. Both Box1 and Y449 are required to signal Bcl2 synthesis and Bax cytosolic retention. This suggests a sequential model in which Jak1, which binds to Box1, is first activated and then phosphorylates Y449, leading to Bcl2 and Bax regulation, accounting for approximately 90% of the survival function. Phosphorylation of Bad required Box1 but not Y449, suggesting that Jak1 also initiates an additional signaling cascade that accounts for approximately 10% of the survival function. Stat5 was activated from the Y449 site but only partially accounted for the survival signal. Proliferation required both Y449 and Box1. Thymocyte development in vivo showed that deletion of Y449 eliminated 90% of alphabeta T-cell development and completely eliminated gammadelta T-cell development, whereas deleting Box 1 completely eliminated both alphabeta and gammadelta T-cell development. Thus the IL-7 receptor controls at least two distinct pathways, in addition to Stat5, that are required for cell survival.

SH-2F LAMPS Instructional Systems Development: Phase II. Final Report.

ERIC Educational Resources Information Center

Gibbons, Andrew S.; Hymes, Jonah P.

This project was one of four aircrew training development projects in a continuing study of the methodology, effectiveness, and resource requirements of the Instructional Systems Development (ISD) process. This report covers the Phase II activities of a two-phase project for the development of aircrew training for SH-2F anti-submarine warfare…

Computer system evolution requirements for autonomous checkout of exploration vehicles

NASA Technical Reports Server (NTRS)

Davis, Tom; Sklar, Mike

1991-01-01

This study, now in its third year, has had the overall objective and challenge of determining the needed hooks and scars in the initial Space Station Freedom (SSF) system to assure that on-orbit assembly and refurbishment of lunar and Mars spacecraft can be accomplished with the maximum use of automation. In this study automation is all encompassing and includes physical tasks such as parts mating, tool operation, and human visual inspection, as well as non-physical tasks such as monitoring and diagnosis, planning and scheduling, and autonomous visual inspection. Potential tasks for automation include both extravehicular activity (EVA) and intravehicular activity (IVA) events. A number of specific techniques and tools have been developed to determine the ideal tasks to be automated, and the resulting timelines, changes in labor requirements and resources required. The Mars/Phobos exploratory mission developed in FY89, and the Lunar Assembly/Refurbishment mission developed in FY90 and depicted in the 90 Day Study as Option 5, have been analyzed in detailed in recent years. The complete methodology and results are presented in FY89 and FY90 final reports.

Activity Theory and the Transformation of Pedagogic Practice

ERIC Educational Resources Information Center

Yamazumi, Katsuhiro

2006-01-01

Today, work and other societal practices are experiencing accelerating paradigm shifts from mass-production-based systems toward new systems based on networking between organizations, collaboration, and partnerships. This shift requires new paradigms in the fields of education, learning, and development. As human activity quickly changes to…

Table-Top Robotics for Engineering Design

ERIC Educational Resources Information Center

Wilczynski, Vincent; Dixon, Gregg; Ford, Eric

2005-01-01

The Mechanical Engineering Section at the U.S. Coast Guard Academy has developed a comprehensive activity based course to introduce second year students to mechanical engineering design. The culminating design activity for the course requires students to design, construct and test robotic devices that complete engineering challenges. Teams of…

Assessing Academic Challenges for Their Contribution to Cognitive Development.

ERIC Educational Resources Information Center

McCormick, David F.; Whittington, M. Susie

2000-01-01

Challenges (assignments, activities, tests) incorporated into agriculture classes were evaluated using Bloom's Taxonomy. The cognitive level varied by course and type of activity. Effectiveness depended on selecting challenges appropriate for the material, requiring higher-order thinking, and rewarding students for work at higher cognitive levels.…

Requirements for Workflow-Based EHR Systems - Results of a Qualitative Study.

PubMed

Schweitzer, Marco; Lasierra, Nelia; Hoerbst, Alexander

2016-01-01

Today's high quality healthcare delivery strongly relies on efficient electronic health records (EHR). These EHR systems or in general healthcare IT-systems are usually developed in a static manner according to a given workflow. Hence, they are not flexible enough to enable access to EHR data and to execute individual actions within a consultation. This paper reports on requirements pointed by experts in the domain of diabetes mellitus to design a system for supporting dynamic workflows to serve personalization within a medical activity. Requirements were collected by means of expert interviews. These interviews completed a conducted triangulation approach, aimed to gather requirements for workflow-based EHR interactions. The data from the interviews was analyzed through a qualitative approach resulting in a set of requirements enhancing EHR functionality from the user's perspective. Requirements were classified according to four different categorizations: (1) process-related requirements, (2) information needs, (3) required functions, (4) non-functional requirements. Workflow related requirements were identified which should be considered when developing and deploying EHR systems.

Autophagy in C. elegans development.

PubMed

Palmisano, Nicholas J; Meléndez, Alicia

2018-04-27

Autophagy involves the sequestration of cytoplasmic contents in a double-membrane structure referred to as the autophagosome and the degradation of its contents upon delivery to lysosomes. Autophagy activity has a role in multiple biological processes during the development of the nematode Caenorhabditis elegans. Basal levels of autophagy are required to remove aggregate prone proteins, paternal mitochondria, and spermatid-specific membranous organelles. During larval development, autophagy is required for the remodeling that occurs during dauer development, and autophagy can selectively degrade components of the miRNA-induced silencing complex, and modulate miRNA-mediated silencing. Basal levels of autophagy are important in synapse formation and in the germ line, to promote the proliferation of proliferating stem cells. Autophagy activity is also required for the efficient removal of apoptotic cell corpses by promoting phagosome maturation. Finally, autophagy is also involved in lipid homeostasis and in the aging process. In this review, we first describe the molecular complexes involved in the process of autophagy, its regulation, and mechanisms for cargo recognition. In the second section, we discuss the developmental contexts where autophagy has been shown to be important. Studies in C. elegans provide valuable insights into the physiological relevance of this process during metazoan development. Copyright © 2018 Elsevier Inc. All rights reserved.

Synthesis of aircraft structures using integrated design and analysis methods

NASA Technical Reports Server (NTRS)

Sobieszczanski-Sobieski, J.; Goetz, R. C.

1978-01-01

A systematic research is reported to develop and validate methods for structural sizing of an airframe designed with the use of composite materials and active controls. This research program includes procedures for computing aeroelastic loads, static and dynamic aeroelasticity, analysis and synthesis of active controls, and optimization techniques. Development of the methods is concerned with the most effective ways of integrating and sequencing the procedures in order to generate structural sizing and the associated active control system, which is optimal with respect to a given merit function constrained by strength and aeroelasticity requirements.

Study of activities of postmen motorcyclists: a look at motorcycle accidents.

PubMed

Nascimento, Lícia Maria Barreto do; Bortolotto, Gracielle Aparecida Orlando

2012-01-01

This article presents the steps of transforming the material and organizational aspects in the work environment, beyond the social order for the proper development of activities in the Household Distribution Center, the Postmen Motorcyclists. The demand was made by managers from the accident records, establishing the need to identify the working conditions of postmen motorcyclists, regarding motorcycle accidents occurring on public roads. Based on the characteristics of the steps required by the methodology, was necessary to realize internal and external observations, with the aim of identifying the dynamics of the accident and the collective dimensions of activity which requires a strategy for monitoring the work on the street.

Using Geo-Data Corporately on the Response Phase of Emergency Management

NASA Astrophysics Data System (ADS)

Demir Ozbek, E.; Ates, S.; Aydinoglu, A. C.

2015-08-01

Response phase of emergency management is the most complex phase in the entire cycle because it requires cooperation between various actors relating to emergency sectors. A variety of geo-data is needed at the emergency response such as; existing data provided by different institutions and dynamic data collected by different sectors at the time of the disaster. Disaster event is managed according to elaborately defined activity-actor-task-geodata cycle. In this concept, every activity of emergency response is determined with Standard Operation Procedure that enables users to understand their tasks and required data in any activity. In this study, a general conceptual approach for disaster and emergency management system is developed based on the regulations to serve applications in Istanbul Governorship Provincial Disaster and Emergency Directorate. The approach is implemented to industrial facility explosion example. In preparation phase, optimum ambulance locations are determined according to general response time of the ambulance to all injury cases in addition to areas that have industrial fire risk. Management of the industrial fire case is organized according to defined actors, activities, and working cycle that describe required geo-data. A response scenario was prepared and performed for an industrial facility explosion event to exercise effective working cycle of actors. This scenario provides using geo-data corporately between different actors while required data for each task is defined to manage the industrial facility explosion event. Following developing web technologies, this scenario based approach can be effective to use geo-data on the web corporately.

Comparison of chemical-use between hydraulic fracturing, acidizing, and routine oil and gas development

DOE PAGES

Stringfellow, William T.; Camarillo, Mary Kay; Domen, Jeremy K.; ...

2017-04-19

The potential hazards and risks associated with well-stimulation in unconventional oil and gas development (hydraulic fracturing, acid fracturing, and matrix acidizing) have been investigated and evaluated and federal and state regulations requiring chemical disclosure for well-stimulation have been implemented as part of an overall risk management strategy for unconventional oil and gas development. Similar evaluations for chemicals used in other routine oil and gas development activities, such as maintenance acidizing, gravel packing, and well drilling, have not been previously conducted, in part due to a lack of reliable information concerning on-field chemical-use. In this study, we compare chemical-use between routinemore » activities and the more closely regulated well-stimulation activities using data collected by the South Coast Air Quality Monitoring District (SCAQMD), which mandates the reporting of both unconventional and routine on-field chemical-use for parts of Southern California. Analysis of this data shows that there is significant overlap in chemical-use between so-called unconventional activities and routine activities conducted for well maintenance, well-completion, or rework. A comparison within the SCAQMD shows a significant overlap between both types and amounts of chemicals used for well-stimulation treatments included under State mandatory-disclosure regulations and routine treatments that are not included under State regulations. A comparison between SCAQMD chemical-use for routine treatments and state-wide chemical-use for hydraulic fracturing also showed close similarity in chemical-use between activities covered under chemical disclosure requirements (e.g. hydraulic fracturing) and many other oil and gas field activities. The results of this study indicate regulations and risk assessments focused exclusively on chemicals used in well-stimulation activities may underestimate potential hazard or risk from overall oil field chemical-use.« less

Comparison of chemical-use between hydraulic fracturing, acidizing, and routine oil and gas development

PubMed Central

Camarillo, Mary Kay; Domen, Jeremy K.; Shonkoff, Seth B. C.

2017-01-01

The potential hazards and risks associated with well-stimulation in unconventional oil and gas development (hydraulic fracturing, acid fracturing, and matrix acidizing) have been investigated and evaluated and federal and state regulations requiring chemical disclosure for well-stimulation have been implemented as part of an overall risk management strategy for unconventional oil and gas development. Similar evaluations for chemicals used in other routine oil and gas development activities, such as maintenance acidizing, gravel packing, and well drilling, have not been previously conducted, in part due to a lack of reliable information concerning on-field chemical-use. In this study, we compare chemical-use between routine activities and the more closely regulated well-stimulation activities using data collected by the South Coast Air Quality Monitoring District (SCAQMD), which mandates the reporting of both unconventional and routine on-field chemical-use for parts of Southern California. Analysis of this data shows that there is significant overlap in chemical-use between so-called unconventional activities and routine activities conducted for well maintenance, well-completion, or rework. A comparison within the SCAQMD shows a significant overlap between both types and amounts of chemicals used for well-stimulation treatments included under State mandatory-disclosure regulations and routine treatments that are not included under State regulations. A comparison between SCAQMD chemical-use for routine treatments and state-wide chemical-use for hydraulic fracturing also showed close similarity in chemical-use between activities covered under chemical disclosure requirements (e.g. hydraulic fracturing) and many other oil and gas field activities. The results of this study indicate regulations and risk assessments focused exclusively on chemicals used in well-stimulation activities may underestimate potential hazard or risk from overall oil field chemical-use. PMID:28422971

Comparison of chemical-use between hydraulic fracturing, acidizing, and routine oil and gas development.

PubMed

Stringfellow, William T; Camarillo, Mary Kay; Domen, Jeremy K; Shonkoff, Seth B C

2017-01-01

The potential hazards and risks associated with well-stimulation in unconventional oil and gas development (hydraulic fracturing, acid fracturing, and matrix acidizing) have been investigated and evaluated and federal and state regulations requiring chemical disclosure for well-stimulation have been implemented as part of an overall risk management strategy for unconventional oil and gas development. Similar evaluations for chemicals used in other routine oil and gas development activities, such as maintenance acidizing, gravel packing, and well drilling, have not been previously conducted, in part due to a lack of reliable information concerning on-field chemical-use. In this study, we compare chemical-use between routine activities and the more closely regulated well-stimulation activities using data collected by the South Coast Air Quality Monitoring District (SCAQMD), which mandates the reporting of both unconventional and routine on-field chemical-use for parts of Southern California. Analysis of this data shows that there is significant overlap in chemical-use between so-called unconventional activities and routine activities conducted for well maintenance, well-completion, or rework. A comparison within the SCAQMD shows a significant overlap between both types and amounts of chemicals used for well-stimulation treatments included under State mandatory-disclosure regulations and routine treatments that are not included under State regulations. A comparison between SCAQMD chemical-use for routine treatments and state-wide chemical-use for hydraulic fracturing also showed close similarity in chemical-use between activities covered under chemical disclosure requirements (e.g. hydraulic fracturing) and many other oil and gas field activities. The results of this study indicate regulations and risk assessments focused exclusively on chemicals used in well-stimulation activities may underestimate potential hazard or risk from overall oil field chemical-use.

Comparison of chemical-use between hydraulic fracturing, acidizing, and routine oil and gas development

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stringfellow, William T.; Camarillo, Mary Kay; Domen, Jeremy K.

The potential hazards and risks associated with well-stimulation in unconventional oil and gas development (hydraulic fracturing, acid fracturing, and matrix acidizing) have been investigated and evaluated and federal and state regulations requiring chemical disclosure for well-stimulation have been implemented as part of an overall risk management strategy for unconventional oil and gas development. Similar evaluations for chemicals used in other routine oil and gas development activities, such as maintenance acidizing, gravel packing, and well drilling, have not been previously conducted, in part due to a lack of reliable information concerning on-field chemical-use. In this study, we compare chemical-use between routinemore » activities and the more closely regulated well-stimulation activities using data collected by the South Coast Air Quality Monitoring District (SCAQMD), which mandates the reporting of both unconventional and routine on-field chemical-use for parts of Southern California. Analysis of this data shows that there is significant overlap in chemical-use between so-called unconventional activities and routine activities conducted for well maintenance, well-completion, or rework. A comparison within the SCAQMD shows a significant overlap between both types and amounts of chemicals used for well-stimulation treatments included under State mandatory-disclosure regulations and routine treatments that are not included under State regulations. A comparison between SCAQMD chemical-use for routine treatments and state-wide chemical-use for hydraulic fracturing also showed close similarity in chemical-use between activities covered under chemical disclosure requirements (e.g. hydraulic fracturing) and many other oil and gas field activities. The results of this study indicate regulations and risk assessments focused exclusively on chemicals used in well-stimulation activities may underestimate potential hazard or risk from overall oil field chemical-use.« less

Hepatic Mitochondrial Pyruvate Carrier 1 Is Required for Efficient Regulation of Gluconeogenesis and Whole-Body Glucose Homeostasis.

PubMed

Gray, Lawrence R; Sultana, Mst Rasheda; Rauckhorst, Adam J; Oonthonpan, Lalita; Tompkins, Sean C; Sharma, Arpit; Fu, Xiaorong; Miao, Ren; Pewa, Alvin D; Brown, Kathryn S; Lane, Erin E; Dohlman, Ashley; Zepeda-Orozco, Diana; Xie, Jianxin; Rutter, Jared; Norris, Andrew W; Cox, James E; Burgess, Shawn C; Potthoff, Matthew J; Taylor, Eric B

2015-10-06

Gluconeogenesis is critical for maintenance of euglycemia during fasting. Elevated gluconeogenesis during type 2 diabetes (T2D) contributes to chronic hyperglycemia. Pyruvate is a major gluconeogenic substrate and requires import into the mitochondrial matrix for channeling into gluconeogenesis. Here, we demonstrate that the mitochondrial pyruvate carrier (MPC) comprising the Mpc1 and Mpc2 proteins is required for efficient regulation of hepatic gluconeogenesis. Liver-specific deletion of Mpc1 abolished hepatic MPC activity and markedly decreased pyruvate-driven gluconeogenesis and TCA cycle flux. Loss of MPC activity induced adaptive utilization of glutamine and increased urea cycle activity. Diet-induced obesity increased hepatic MPC expression and activity. Constitutive Mpc1 deletion attenuated the development of hyperglycemia induced by a high-fat diet. Acute, virally mediated Mpc1 deletion after diet-induced obesity decreased hyperglycemia and improved glucose tolerance. We conclude that the MPC is required for efficient regulation of gluconeogenesis and that the MPC contributes to the elevated gluconeogenesis and hyperglycemia in T2D. Copyright © 2015 Elsevier Inc. All rights reserved.

Survey of RFID and Its Application to International Ocean/Air Container Tracking

NASA Astrophysics Data System (ADS)

Katayama, Minoru; Nakada, Hiroshi; Hayashi, Hitoshi; Shimizu, Masashi

“Internet of Things” (IoT) requires information to be collected from “anything”, “anytime”, and “anywhere”. In order to achieve this, wireless devices are required that have (1) automatic data acquisition capability, (2) small size, (3) long life, and (4) long range communication capability. One way to meet these requirements is to adopt active Radio Frequency Identification (RFID) systems. Active RFID is more advantageous than passive RFID and enables higher data reading performance over longer distances. This paper surveys active RFID systems, the services they currently promise to provide, technical problems common to these services, and the direction in which research should head in the future. It also reports the results of EPCglobal (EPC: Electronic Product Code) pilot tests conducted on global logistics for tracking ocean/air container transportation using active RFID systems for which we developed several new types of active RFID tags. The test results confirm that our active RFID tags have sufficient capability and low power consumption to well support ocean/air transportation and logistics service.

A format for the interchange of scheduling models

NASA Technical Reports Server (NTRS)

Jaap, John P.; Davis, Elizabeth K.

1994-01-01

In recent years a variety of space-activity schedulers have been developed within the aerospace community. Space-activity schedulers are characterized by their need to handle large numbers of activities which are time-window constrained and make high demands on many scarce resources, but are minimally constrained by predecessor/successor requirements or critical paths. Two needs to exchange data between these schedulers have materialized. First, there is significant interest in comparing and evaluating the different scheduling engines to ensure that the best technology is applied to each scheduling endeavor. Second, there is a developing requirement to divide a single scheduling task among different sites, each using a different scheduler. In fact, the scheduling task for International Space Station Alpha (ISSA) will be distributed among NASA centers and among the international partners. The format used to interchange scheduling data for ISSA will likely use a growth version of the format discussed in this paper. The model interchange format (or MIF, pronounced as one syllable) discussed in this paper is a robust solution to the need to interchange scheduling requirements for space activities. It is highly extensible, human-readable, and can be generated or edited with common text editors. It also serves well the need to support a 'benchmark' data case which can be delivered on any computer platform.

22 CFR 122.1 - Registration requirements.

Code of Federal Regulations, 2010 CFR

2010-04-01

... official capacity. (2) Persons whose pertinent business activity is confined to the production of... articles for experimental or scientific purpose, including research and development. (c) Purpose...

Human Factors Operability Timeline Analysis to Improve the Processing Flow of the Orion Spacecraft

NASA Technical Reports Server (NTRS)

Stambolian, Damon B.; Schlierf, Roland; Miller, Darcy; Posada, Juan; Haddock, Mike; Haddad, Mike; Tran, Donald; Henderon, Gena; Barth, Tim

2011-01-01

This slide presentation reviews the use of Human factors and timeline analysis to have a more efficient and effective processing flow. The solution involved developing a written timeline of events that included each activity within each functional flow block. Each activity had computer animation videos and pictures of the people involved and the hardware. The Human Factors Engineering Analysis Tool (HFEAT) was improved by modifying it to include the timeline of events. The HFEAT was used to define the human factors requirements and design solutions were developed for these requirements. An example of a functional flow block diagram is shown, and a view from one of the animations (i.e., short stack pallet) is shown and explained.

Virtual prototyping of a semi-active transfemoral prosthetic leg.

PubMed

Lui, Zhen Wei; Awad, Mohammed I; Abouhossein, Alireza; Dehghani-Sanij, Abbas A; Messenger, Neil

2015-05-01

This article presents a virtual prototyping study of a semi-active lower limb prosthesis to improve the functionality of an amputee during prosthesis-environment interaction for level ground walking. Articulated ankle-foot prosthesis and a single-axis semi-active prosthetic knee with active and passive operating modes were considered. Data for level ground walking were collected using a photogrammetric method in order to develop a base-line simulation model and with the hip kinematics input to verify the proposed design. The simulated results show that the semi-active lower limb prosthesis is able to move efficiently in passive mode, and the activation time of the knee actuator can be reduced by approximately 50%. Therefore, this semi-active system has the potential to reduce the energy consumption of the actuators required during level ground walking and requires less compensation from the amputee due to lower deviation of the vertical excursion of body centre of mass. © IMechE 2015.

Definition of technology development missions for early space stations orbit transfer vehicle serving. Phase 2, task 1: Space station support of operational OTV servicing

NASA Technical Reports Server (NTRS)

1983-01-01

Representative space based orbital transfer vehicles (OTV), ground based vehicle turnaround assessment, functional operational requirements and facilities, mission turnaround operations, a comparison of ground based versus space based tasks, activation of servicing facilities prior to IOC, fleet operations requirements, maintenance facilities, OTV servicing facilities, space station support requirements, and packaging for delivery are discussed.

A Handful of Bacteria: A Simple Activity That Engages Students to Think and Write Like a Scientist

ERIC Educational Resources Information Center

Song, Pengfei

2014-01-01

Non-major students taking science courses to fulfill degree requirements may show low interest in traditional lecture-based classrooms. While professors may try to incorporate hands-on activities to the classroom to foster enthusiasm, the development of a low-cost, low-hazard scienitfic activity that engages students can be challenging. Moreover,…

10 CFR 473.10 - Required information from applicant.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION AUTOMOTIVE PROPULSION RESEARCH AND DEVELOPMENT Review and... automobile propulsion system shall— (a) State whether the activities will initiate or continue research and development of an advanced automobile propulsion system; (b) State, insofar as the applicant has information...

10 CFR 473.10 - Required information from applicant.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION AUTOMOTIVE PROPULSION RESEARCH AND DEVELOPMENT Review and... automobile propulsion system shall— (a) State whether the activities will initiate or continue research and development of an advanced automobile propulsion system; (b) State, insofar as the applicant has information...

Annual report of Iowa Highway Research Board research and development activities FY 2010.

DOT National Transportation Integrated Search

2010-12-01

The Highway Division of the Iowa Department of Transportation (Iowa DOT) engages in research and : development for two reasons: first, to find workable solutions to the many problems that require more than : ordinary, routine investigation; and secon...

10 CFR 473.10 - Required information from applicant.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION AUTOMOTIVE PROPULSION RESEARCH AND DEVELOPMENT Review and... automobile propulsion system shall— (a) State whether the activities will initiate or continue research and development of an advanced automobile propulsion system; (b) State, insofar as the applicant has information...

Loss of Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) Reveals a Requirement for MAPK Signalling in Mouse Sex Determination

PubMed Central

Bogani, Debora; Siggers, Pam; Brixey, Rachel; Warr, Nick; Beddow, Sarah; Edwards, Jessica; Williams, Debbie; Wilhelm, Dagmar; Koopman, Peter; Flavell, Richard A.; Chi, Hongbo; Ostrer, Harry; Wells, Sara; Cheeseman, Michael; Greenfield, Andy

2009-01-01

Sex determination in mammals is controlled by the presence or absence of the Y-linked gene SRY. In the developing male (XY) gonad, sex-determining region of the Y (SRY) protein acts to up-regulate expression of the related gene, SOX9, a transcriptional regulator that in turn initiates a downstream pathway of testis development, whilst also suppressing ovary development. Despite the requirement for a number of transcription factors and secreted signalling molecules in sex determination, intracellular signalling components functioning in this process have not been defined. Here we report a role for the phylogenetically ancient mitogen-activated protein kinase (MAPK) signalling pathway in mouse sex determination. Using a forward genetic screen, we identified the recessive boygirl (byg) mutation. On the C57BL/6J background, embryos homozygous for byg exhibit consistent XY gonadal sex reversal. The byg mutation is an A to T transversion causing a premature stop codon in the gene encoding MAP3K4 (also known as MEKK4), a mitogen-activated protein kinase kinase kinase. Analysis of XY byg/byg gonads at 11.5 d post coitum reveals a growth deficit and a failure to support mesonephric cell migration, both early cellular processes normally associated with testis development. Expression analysis of mutant XY gonads at the same stage also reveals a dramatic reduction in Sox9 and, crucially, Sry at the transcript and protein levels. Moreover, we describe experiments showing the presence of activated MKK4, a direct target of MAP3K4, and activated p38 in the coelomic region of the XY gonad at 11.5 d post coitum, establishing a link between MAPK signalling in proliferating gonadal somatic cells and regulation of Sry expression. Finally, we provide evidence that haploinsufficiency for Map3k4 accounts for T-associated sex reversal (Tas). These data demonstrate that MAP3K4-dependent signalling events are required for normal expression of Sry during testis development, and create a novel entry point into the molecular and cellular mechanisms underlying sex determination in mice and disorders of sexual development in humans. PMID:19753101

Model Wellness Policy

ERIC Educational Resources Information Center

South Dakota Department of Education, 2005

2005-01-01

In the Child Nutrition and WIC Reauthorization Act of 2004, PL 105-268, the U.S. Congress established a new requirement for all local agencies with a federally funded National School Lunch program. The local agencies are required to develop and implement wellness policies that address nutrition and physical activity by the start of the 2006-07…

78 FR 42957 - Agency Information Collection Activities: Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-18

... Drug Coverage; Use: Section 1860D-4(g)(1) of the Social Security Act, requires that Part D plan... and D Universal Audit Guide; Use: Under the Medicare Prescription Drug, Improvement, and Modernization... requirements. In 2010 the explosive growth of these sponsoring organizations forced us to develop an audit...

Creating a mission-based reporting system at an academic health center.

PubMed

Howell, Lydia Pleotis; Hogarth, Michael; Anders, Thomas F

2002-02-01

The authors developed a Web-based mission-based reporting (MBR) system for their university's (UC Davis's) health system to report faculty members' activities in research and creative work, clinical service, education, and community/university service. They developed the system over several years (1998-2001) in response to a perceived need to better define faculty members' productivity for faculty development, financial management, and program assessment. The goal was to create a measurement tool that could be used by department chairs to counsel faculty on their performances. The MBR system provides measures of effort for each of the university's four missions. Departments or the school can use the output to better define expenditures and allocations of resources. The system provides both a quantitative metric of times spent on various activities within each mission, and a qualitative metric for the effort expended. The authors report the process of developing the MBR system and making it applicable for both clinical and basic science departments, and the mixed success experienced in its implementation. The system appears to depict the activities of most faculty fairly accurately, and chairs of test departments have been generally enthusiastic. However, resistance to general implementation remains, chiefly due to concerns about reliability, validity, and time required for completing the report. The authors conclude that MBR can be useful but will require some streamlining and the elimination of other redundant reporting instruments. A well-defined purpose is required to motivate its use.

Direct and indirect requirements of Shh/Gli signaling in early pituitary development.

PubMed

Wang, Yiwei; Martin, James F; Bai, C Brian

2010-12-15

Induction of early pituitary progenitors is achieved through combined activities of signals from adjacent embryonic tissues. Previous studies have identified a requirement for oral ectoderm derived Sonic Hedgehog (Shh) in specification and/or proliferation of early pituitary progenitors, however how different Gli genes mediate Shh signaling to control pituitary progenitor development has not yet been determined. Here we show that Gli2, which encodes a major Gli activator, is required for proliferation of specific groups of pituitary progenitors but not for initial dorsoventral patterning. We further show that the action of Gli2 occurs prior to the closure of Rathke' pouch. Lastly, we show that Shh/Gli2 signaling controls the diencephalic expression of Bone morphogenetic protein 4 (Bmp4) and Fibroblast growth factor 8 (Fgf8), two genes that are known to play critical roles in patterning and growth of Rathke's pouch. Our results therefore suggest both cell-autonomous and non-cell-autonomous requirements for Gli2 in regulation of pituitary progenitor specification, proliferation and differentiation. Copyright © 2010 Elsevier Inc. All rights reserved.

Policies for promotion of physical activity and prevention of obesity in adolescence.

PubMed

Pate, Russell R; Flynn, Jennifer I; Dowda, Marsha

2016-12-01

Obesity rates among children and adolescents in developed countries have increased dramatically since the 1970s. During that same period, numerous secular changes have combined to reduce the demand for physical activity in day-to-day life, and many barriers to physical activity are now evident. As a consequence, most children and adolescents do not meet the accepted public health guidelines for physical activity. Accordingly, public health interventions are needed to increase physical activity in adolescence. Such interventions, if successfully implemented, can be expected to improve fitness and health as well as reduce the prevalence of overweight and obesity in young people. Promotion of physical activity in populations of children and adolescents will require comprehensive strategic planning and adoption of new policies in multiple societal sectors. This paper highlights nine initiatives that can address the problem of physical activity in children. The initiatives are to: establish comprehensive school physical activity programming; demand high quality physical education; require physical activity in early child care and education; require physical activity in afterschool programs; create equity in community resources; activate youth sports programs; re-normalize active transport to school; institutionalize clinic-based physical activity assessment and counseling; and build activity-friendly homes. A case will be made for comprehensive national and international strategic planning aimed at effective and large-scale implementation of these initiatives and tactics.

Tight regulation of p53 activity by Mdm2 is required for ureteric bud growth and branching

PubMed Central

Hilliard, Sylvia; Aboudehen, Karam; Yao, Xiao; El-Dahr, Samir S.

2011-01-01

Mdm2 (Murine Double Minute-2) is required to control cellular p53 activity and protein levels. Mdm2 null embryos die of p53-mediated growth arrest and apoptosis at the peri-implantation stage. Thus, the absolute requirement for Mdm2 in organogenesis is unknown. This study examined the role of Mdm2 in kidney development, an organ which develops via epithelial-mesenchymal interactions and branching morphogenesis. Mdm2 mRNA and protein are expressed in the ureteric bud (UB) epithelium and metanephric mesenchyme (MM) lineages. We report here the results of conditional deletion of Mdm2 from the UB epithelium. UBmdm2−/− mice die soon after birth and uniformly display severe renal hypodysplasia due to defective UB branching and underdeveloped nephrogenic zone. Ex vivo cultured UBmdm2−/− explants exhibit arrested development of the UB and its branches and consequently develop few nephron progenitors. UBmdm2−/− cells have reduced proliferation rate and enhanced apoptosis. Although markedly reduced in number, the UB tips of UBmdm2−/− metanephroi continue to express c-ret and Wnt11; however, there was a notable reduction in Wnt9b, Lhx-1 and Pax-2 expression levels. We further show that the UBmdm2−/− mutant phenotype is mediated by aberrant p53 activity because it is rescued by UB-specific deletion of the p53 gene. These results demonstrate a critical and cell autonomous role for Mdm2 in the UB lineage. Mdm2-mediated inhibition of p53 activity is a prerequisite for renal organogenesis. PMID:21420949

Development of Entry-Level Competence Tests: A Strategy for Evaluation of Vocational Education Training Systems

ERIC Educational Resources Information Center

Schutte, Marc; Spottl, Georg

2011-01-01

Developing countries such as Malaysia and Oman have recently established occupational standards based on core work processes (functional clusters of work objects, activities and performance requirements), to which competencies (performance determinants) can be linked. While the development of work-process-based occupational standards is supposed…

Evaluating a Professional Development Framework to Empower Chemistry Teachers to Design Context-Based Education

ERIC Educational Resources Information Center

Stolk, Machiel Johan; Bulte, Astrid; De Jong, Onno; Pilot, Albert

2012-01-01

Even experienced chemistry teachers require professional development when they are encouraged to become actively engaged in the design of new context-based education. This study briefly describes the development of a framework consisting of goals, learning phases, strategies and instructional functions, and how the framework was translated into a…

EVA Communications Avionics and Informatics

NASA Technical Reports Server (NTRS)

Carek, David Andrew

2005-01-01

The Glenn Research Center is investigating and developing technologies for communications, avionics, and information systems that will significantly enhance extra vehicular activity capabilities to support the Vision for Space Exploration. Several of the ongoing research and development efforts are described within this presentation including system requirements formulation, technology development efforts, trade studies, and operational concept demonstrations.

Aerospace Engineering Systems

NASA Technical Reports Server (NTRS)

VanDalsem, William R.; Livingston, Mary E.; Melton, John E.; Torres, Francisco J.; Stremel, Paul M.

1999-01-01

Continuous improvement of aerospace product development processes is a driving requirement across much of the aerospace community. As up to 90% of the cost of an aerospace product is committed during the first 10% of the development cycle, there is a strong emphasis on capturing, creating, and communicating better information (both requirements and performance) early in the product development process. The community has responded by pursuing the development of computer-based systems designed to enhance the decision-making capabilities of product development individuals and teams. Recently, the historical foci on sharing the geometrical representation and on configuration management are being augmented: Physics-based analysis tools for filling the design space database; Distributed computational resources to reduce response time and cost; Web-based technologies to relieve machine-dependence; and Artificial intelligence technologies to accelerate processes and reduce process variability. Activities such as the Advanced Design Technologies Testbed (ADTT) project at NASA Ames Research Center study the strengths and weaknesses of the technologies supporting each of these trends, as well as the overall impact of the combination of these trends on a product development event. Lessons learned and recommendations for future activities will be reported.

Osteoblast differentiation and skeletal development are regulated by Mdm2–p53 signaling

PubMed Central

Lengner, Christopher J.; Steinman, Heather A.; Gagnon, James; Smith, Thomas W.; Henderson, Janet E.; Kream, Barbara E.; Stein, Gary S.; Lian, Jane B.; Jones, Stephen N.

2006-01-01

Mdm2 is required to negatively regulate p53 activity at the peri-implantation stage of early mouse development. However, the absolute requirement for Mdm2 throughout embryogenesis and in organogenesis is unknown. To explore Mdm2–p53 signaling in osteogenesis, Mdm2-conditional mice were bred with Col3.6-Cre–transgenic mice that express Cre recombinase in osteoblast lineage cells. Mdm2-conditional Col3.6-Cre mice die at birth and display multiple skeletal defects. Osteoblast progenitor cells deleted for Mdm2 have elevated p53 activity, reduced proliferation, reduced levels of the master osteoblast transcriptional regulator Runx2, and reduced differentiation. In contrast, p53-null osteoprogenitor cells have increased proliferation, increased expression of Runx2, increased osteoblast maturation, and increased tumorigenic potential, as mice specifically deleted for p53 in osteoblasts develop osteosarcomas. These results demonstrate that p53 plays a critical role in bone organogenesis and homeostasis by negatively regulating bone development and growth and by suppressing bone neoplasia and that Mdm2-mediated inhibition of p53 function is a prerequisite for Runx2 activation, osteoblast differentiation, and proper skeletal formation. PMID:16533949

Manned geosynchronous mission requirements and systems analysis study extension. Volume 1: Executive summary

NASA Technical Reports Server (NTRS)

1981-01-01

A study was performed to determine the types of manned missions that will likely be performed in the late 1980's or early 1990's timeframe, to define MOTV configurations which satisfy these missions requirements, and to develop a program plan for its development. Twenty generic missions were originally defined for MOTV but, to simplify the selection process, five of these missions were selected as typical and used as Design Reference Missions. Systems and subsystems requirements were re-examined and sensitivity analyses performed to determine optimum point designs. Turnaround modes were considered to determine the most effective combination of ground based and spaced based activities. A preferred concept for the crew capsule and for the mission mode was developed.

Symbol Sense Behavior in Digital Activities

ERIC Educational Resources Information Center

Bokhove, Christian; Drijvers, Paul

2010-01-01

The algebraic expertise that mathematics education is aiming for includes both procedural skills and conceptual understanding. To capture the latter, notions such as symbol sense, gestalt view and visual salience have been developed. We wonder if digital activities can be designed that not only require procedural algebraic skills, but also invite…

Investigating the Relationship among Extracurricular Activities, Learning Approach and Academic Outcomes: A Case Study

ERIC Educational Resources Information Center

Chan, Yiu-Kong

2016-01-01

Learning effectiveness requires an understanding of the relationship among extracurricular activities, learning approach and academic performance and, it is argued, this helps educators develop techniques designed to enrich learning effectiveness. Biggs' Presage-Process-Product model on student learning has identified the relationship among…

BONES, TEACHER'S GUIDE.

ERIC Educational Resources Information Center

Elementary Science Study, Newton, MA.

THIS GUIDE WAS DEVELOPED FOR USE WITH THE ELEMENTARY SCIENCE STUDY UNIT ON "BONES.""BONES" HAS BEEN TAUGHT IN THE FOURTH GRADE AND REQUIRES FROM 10 TO 25 LESSONS, DEPENDING ON THE NUMBER OF ACTIVITIES USED. THE GUIDE DOES NOT PROVIDE DETAILED INSTRUCTION FOR CONDUCTING CLASSES, BUT RATHER SOME POSSIBLE ACTIVITIES, AND LEAVES…

Psychological Benefits of Regular Physical Activity: Evidence from Emerging Adults

ERIC Educational Resources Information Center

Cekin, Resul

2015-01-01

Emerging adulthood is a transitional stage between late adolescence and young adulthood in life-span development that requires significant changes in people's lives. Therefore, identifying protective factors for this population is crucial. This study investigated the effects of regular physical activity on self-esteem, optimism, and happiness in…

Personal Values and Mission Statement: A Reflective Activity to Aid Moral Development

ERIC Educational Resources Information Center

Laird-Magee, Tyler; Gayle, Barbra Mae; Preiss, Raymond

2015-01-01

Personal values guide ethical decision-making behaviors. Business professors have traditionally addressed undergraduate ethics-based learning through a learn ethics approach using case studies, simulations, presentations, and other activities. Few offer a live ethics orientation requiring completion of a personal values self-assessment and…

14 CFR § 1232.107 - Sanctions.

Code of Federal Regulations, 2014 CFR

2014-01-01

....nasa.gov/; and (b) NASA Procedural Requirements (NPR) 8910.1, Care and Use of Animals, delineates the... hardware development activities. NPR 8910.1 is accessible is access at http://nodis3.gsfc.nasa.gov/. ... NASA ACTIVITIES (Eff. until 2-14-14) § 1232.107 Sanctions. (a) Non-NASA Institutions. Principal...

50 CFR 218.174 - Requirements for monitoring and reporting.

Code of Federal Regulations, 2013 CFR

2013-10-01

...-based surveys shall be designed to maximize detections of marine mammals near mission activity event. (2... IMPORTING OF MARINE MAMMALS Taking Marine Mammals Incidental to U.S. Navy Research, Development, Test, and... Navy to implement, at a minimum, the monitoring activities summarized below: (1) Visual Surveys: (i...

50 CFR 218.174 - Requirements for monitoring and reporting.

Code of Federal Regulations, 2014 CFR

2014-10-01

...-based surveys shall be designed to maximize detections of marine mammals near mission activity event. (2... IMPORTING OF MARINE MAMMALS Taking Marine Mammals Incidental to U.S. Navy Research, Development, Test, and... Navy to implement, at a minimum, the monitoring activities summarized below: (1) Visual Surveys: (i...

50 CFR 218.174 - Requirements for monitoring and reporting.

Code of Federal Regulations, 2012 CFR

2012-10-01

...-based surveys shall be designed to maximize detections of marine mammals near mission activity event. (2... IMPORTING OF MARINE MAMMALS Taking Marine Mammals Incidental to U.S. Navy Research, Development, Test, and... Navy to implement, at a minimum, the monitoring activities summarized below: (1) Visual Surveys: (i...

Development of brain networks involved in spoken word processing of Mandarin Chinese.

PubMed

Cao, Fan; Khalid, Kainat; Lee, Rebecca; Brennan, Christine; Yang, Yanhui; Li, Kuncheng; Bolger, Donald J; Booth, James R

2011-08-01

Developmental differences in phonological and orthographic processing of Chinese spoken words were examined in 9-year-olds, 11-year-olds and adults using functional magnetic resonance imaging (fMRI). Rhyming and spelling judgments were made to two-character words presented sequentially in the auditory modality. Developmental comparisons between adults and both groups of children combined showed that age-related changes in activation in visuo-orthographic regions depended on a task. There were developmental increases in the left inferior temporal gyrus and the right inferior occipital gyrus in the spelling task, suggesting more extensive visuo-orthographic processing in a task that required access to these representations. Conversely, there were developmental decreases in activation in the left fusiform gyrus and left middle occipital gyrus in the rhyming task, suggesting that the development of reading is marked by reduced involvement of orthography in a spoken language task that does not require access to these orthographic representations. Developmental decreases may arise from the existence of extensive homophony (auditory words that have multiple spellings) in Chinese. In addition, we found that 11-year-olds and adults showed similar activation in the left superior temporal gyrus across tasks, with both groups showing greater activation than 9-year-olds. This pattern suggests early development of perceptual representations of phonology. In contrast, 11-year-olds and 9-year-olds showed similar activation in the left inferior frontal gyrus across tasks, with both groups showing weaker activation than adults. This pattern suggests late development of controlled retrieval and selection of lexical representations. Altogether, this study suggests differential effects of character acquisition on development of components of the language network in Chinese as compared to previous reports on alphabetic languages. Published by Elsevier Inc.

The ITER bolometer diagnostic: Status and plansa)

NASA Astrophysics Data System (ADS)

Meister, H.; Giannone, L.; Horton, L. D.; Raupp, G.; Zeidner, W.; Grunda, G.; Kalvin, S.; Fischer, U.; Serikov, A.; Stickel, S.; Reichle, R.

2008-10-01

A consortium consisting of four EURATOM Associations has been set up to develop the project plan for the full development of the ITER bolometer diagnostic and to continue urgent R&D activities. An overview of the current status is given, including detector development, line-of-sight optimization, performance analysis as well as the design of the diagnostic components and their integration in ITER. This is complemented by the presentation of plans for future activities required to successfully implement the bolometer diagnostic, ranging from the detector development over diagnostic design and prototype testing to RH tools for calibration.

Towards a Methodology for Identifying Program Constraints During Requirements Analysis

NASA Technical Reports Server (NTRS)

Romo, Lilly; Gates, Ann Q.; Della-Piana, Connie Kubo

1997-01-01

Requirements analysis is the activity that involves determining the needs of the customer, identifying the services that the software system should provide and understanding the constraints on the solution. The result of this activity is a natural language document, typically referred to as the requirements definition document. Some of the problems that exist in defining requirements in large scale software projects includes synthesizing knowledge from various domain experts and communicating this information across multiple levels of personnel. One approach that addresses part of this problem is called context monitoring and involves identifying the properties of and relationships between objects that the system will manipulate. This paper examines several software development methodologies, discusses the support that each provide for eliciting such information from experts and specifying the information, and suggests refinements to these methodologies.

Impact of low gravity on water electrolysis operation

NASA Technical Reports Server (NTRS)

Powell, F. T.; Schubert, F. H.; Lee, M. G.

1989-01-01

Advanced space missions will require oxygen and hydrogen utilities for several important operations including the following: (1) propulsion; (2) electrical power generation and storage; (3) environmental control and life support; (4) extravehicular activity; (5) in-space manufacturing and (6) in-space science activities. An experiment suited to a Space Shuttle standard middeck payload has been designed for the Static Feed Water Electrolysis technology which has been viewed as being capable of efficient, reliable oxygen and hydrogen generation with few subsystem components. The program included: end use design requirements, phenomena to be studied, Space Shuttle Orbiter experiment constraints, experiment design and data requirements, and test hardware requirements. The objectives are to obtain scientific and engineering data for future research and development and to focus on demonstrating and monitoring for safety of a standard middeck payload.

GSFC Information Systems Technology Developments Supporting the Vision for Space Exploration

NASA Technical Reports Server (NTRS)

Hughes, Peter; Dennehy, Cornelius; Mosier, Gary; Smith, Dan; Rykowski, Lisa

2004-01-01

The Vision for Space Exploration will guide NASA's future human and robotic space activities. The broad range of human and robotic missions now being planned will require the development of new system-level capabilities enabled by emerging new technologies. Goddard Space Flight Center is actively supporting the Vision for Space Exploration in a number of program management, engineering and technology areas. This paper provides a brief background on the Vision for Space Exploration and a general overview of potential key Goddard contributions. In particular, this paper focuses on describing relevant GSFC information systems capabilities in architecture development; interoperable command, control and communications; and other applied information systems technology/research activities that are applicable to support the Vision for Space Exploration goals. Current GSFC development efforts and task activities are presented together with future plans.

Engaging students as partners in developing online learning and feedback activities for first-year fluid mechanics

NASA Astrophysics Data System (ADS)

Brown, Alan

2018-01-01

Much learning takes place outside of formal class settings, yet students starting in higher education are not always well equipped with independent learning skills, appropriate self-knowledge or the required levels of intrinsic motivation This project used students as partners to develop resources that could be used by first-year undergraduates in fluid mechanics, using activities and receiving feedback through the virtual learning environment (VLE), in order to build these three attributes of independent learners. While there were significant benefits to the students who developed the resources, the target students saw much lower benefits as a result of poorer than expected engagement. The challenge this research presents is to develop activities that maximise engagement in large classes, as well as develop appropriate independent learning skills.

HIS priorities in developing countries.

PubMed

Amado Espinosa, L

1995-04-01

Looking for a solution to fulfill the requirements that the new global economical system demands, developing countries face a reality of poor communications infrastructure, a delay in applying information technology to the organizations, and a semi-closed political system avoiding the necessary reforms. HIS technology has been developed more for transactional purposes on mini and mainframe platforms. Administrative modules are the most frequently observed and physicians are now requiring more support for their activities. The second information systems generation will take advantage of PC technology, client-server models and telecommunications to achieve integration. International organizations, academic and industrial, public and private, will play a major role to transfer technology and to develop this area.

Requirements for Medical Modeling Languages

PubMed Central

van der Maas, Arnoud A.F.; Ter Hofstede, Arthur H.M.; Ten Hoopen, A. Johannes

2001-01-01

Objective: The development of tailor-made domain-specific modeling languages is sometimes desirable in medical informatics. Naturally, the development of such languages should be guided. The purpose of this article is to introduce a set of requirements for such languages and show their application in analyzing and comparing existing modeling languages. Design: The requirements arise from the practical experience of the authors and others in the development of modeling languages in both general informatics and medical informatics. The requirements initially emerged from the analysis of information modeling techniques. The requirements are designed to be orthogonal, i.e., one requirement can be violated without violation of the others. Results: The proposed requirements for any modeling language are that it be “formal” with regard to syntax and semantics, “conceptual,” “expressive,” “comprehensible,” “suitable,” and “executable.” The requirements are illustrated using both the medical logic modules of the Arden Syntax as a running example and selected examples from other modeling languages. Conclusion: Activity diagrams of the Unified Modeling Language, task structures for work flows, and Petri nets are discussed with regard to the list of requirements, and various tradeoffs are thus made explicit. It is concluded that this set of requirements has the potential to play a vital role in both the evaluation of existing domain-specific languages and the development of new ones. PMID:11230383

Automated segmentation of blood-flow regions in large thoracic arteries using 3D-cine PC-MRI measurements.

PubMed

van Pelt, Roy; Nguyen, Huy; ter Haar Romeny, Bart; Vilanova, Anna

2012-03-01

Quantitative analysis of vascular blood flow, acquired by phase-contrast MRI, requires accurate segmentation of the vessel lumen. In clinical practice, 2D-cine velocity-encoded slices are inspected, and the lumen is segmented manually. However, segmentation of time-resolved volumetric blood-flow measurements is a tedious and time-consuming task requiring automation. Automated segmentation of large thoracic arteries, based solely on the 3D-cine phase-contrast MRI (PC-MRI) blood-flow data, was done. An active surface model, which is fast and topologically stable, was used. The active surface model requires an initial surface, approximating the desired segmentation. A method to generate this surface was developed based on a voxel-wise temporal maximum of blood-flow velocities. The active surface model balances forces, based on the surface structure and image features derived from the blood-flow data. The segmentation results were validated using volunteer studies, including time-resolved 3D and 2D blood-flow data. The segmented surface was intersected with a velocity-encoded PC-MRI slice, resulting in a cross-sectional contour of the lumen. These cross-sections were compared to reference contours that were manually delineated on high-resolution 2D-cine slices. The automated approach closely approximates the manual blood-flow segmentations, with error distances on the order of the voxel size. The initial surface provides a close approximation of the desired luminal geometry. This improves the convergence time of the active surface and facilitates parametrization. An active surface approach for vessel lumen segmentation was developed, suitable for quantitative analysis of 3D-cine PC-MRI blood-flow data. As opposed to prior thresholding and level-set approaches, the active surface model is topologically stable. A method to generate an initial approximate surface was developed, and various features that influence the segmentation model were evaluated. The active surface segmentation results were shown to closely approximate manual segmentations.

Uncertainty Quantification Techniques for Population Density Estimates Derived from Sparse Open Source Data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stewart, Robert N; White, Devin A; Urban, Marie L

2013-01-01

The Population Density Tables (PDT) project at the Oak Ridge National Laboratory (www.ornl.gov) is developing population density estimates for specific human activities under normal patterns of life based largely on information available in open source. Currently, activity based density estimates are based on simple summary data statistics such as range and mean. Researchers are interested in improving activity estimation and uncertainty quantification by adopting a Bayesian framework that considers both data and sociocultural knowledge. Under a Bayesian approach knowledge about population density may be encoded through the process of expert elicitation. Due to the scale of the PDT effort whichmore » considers over 250 countries, spans 40 human activity categories, and includes numerous contributors, an elicitation tool is required that can be operationalized within an enterprise data collection and reporting system. Such a method would ideally require that the contributor have minimal statistical knowledge, require minimal input by a statistician or facilitator, consider human difficulties in expressing qualitative knowledge in a quantitative setting, and provide methods by which the contributor can appraise whether their understanding and associated uncertainty was well captured. This paper introduces an algorithm that transforms answers to simple, non-statistical questions into a bivariate Gaussian distribution as the prior for the Beta distribution. Based on geometric properties of the Beta distribution parameter feasibility space and the bivariate Gaussian distribution, an automated method for encoding is developed that responds to these challenging enterprise requirements. Though created within the context of population density, this approach may be applicable to a wide array of problem domains requiring informative priors for the Beta distribution.« less

Sensation in a single neuron pair represses male behavior in hermaphrodites

PubMed Central

White, Jamie Q.; Jorgensen, Erik M.

2012-01-01

Summary Pheromones elicit innate sex-specific mating behaviors in many species. We demonstrate that in C. elegans, male-specific sexual attraction behavior is programmed in both sexes but repressed in hermaphrodites. Repression requires a single sensory neuron pair, the ASIs. To represses attraction in adults, the ASIs must be present, active, and capable of sensing the environment during development. The ASIs release TGF-β, and ASI function can be bypassed by experimental activation of TGF-β signaling. Sexual attraction in de-repressed hermaphrodites requires the same sensory neurons as in males. The sexual identity of both these sensory neurons and a distinct subset of interneurons must be male to relieve repression and release attraction. TGF-β may therefore act to change connections between sensory- and interneurons during development to engage repression. Thus, sensation in a single sensory neuron pair during development reprograms a common neural circuit from male to female behavior. PMID:22920252

Constitutive activation of NOTCH1 signaling in Sertoli cells causes gonocyte exit from quiescence

PubMed Central

Garcia, Thomas Xavier; DeFalco, Tony; Capel, Blanche; Hofmann, Marie-Claude

2013-01-01

Notch signaling components have long been detected in Sertoli and germ cells in the developing and mature testis. However, the role of this pathway in testis development and spermatogenesis remains unknown. Using reporter mice expressing green fluorescent protein following Notch receptor activation, we found that Notch signaling was active in Sertoli cells at various fetal, neonatal, and adult stages. Since Notch signaling specifies stem cell fate in many developing and mature organ systems, we hypothesized that maintenance and differentiation of gonocytes and/or spermatogonial stem cells would be modulated through this pathway in Sertoli cells. To this end, we generated mutant mice constitutively expressing the active, intracellular domain of NOTCH1 (NICD1) in Sertoli cells. We found that mutant Sertoli cells were morphologically normal before and after birth, but presented a number of functional changes that drastically affected gonocyte numbers and physiology. We observed aberrant exit of gonocytes from mitotic arrest, migration toward cord periphery, and premature differentiation before birth. These events, presumably unsupported by the cellular microenvironment, were followed by gonocyte apoptosis and near complete disappearance of the gonocytes by day 2 after birth. Molecular analysis demonstrated that these effects are correlated with a dysregulation of Sertoli-expressed genes that are required for germ cell maintenance, such as Cyp26b1 and Gdnf. Taken together, our results demonstrate that Notch signaling is active in Sertoli cells throughout development and that proper regulation of Notch signaling in Sertoli cells is required for the maintenance of gonocytes in an undifferentiated state during fetal development. PMID:23391689

Designing eHealth that Matters via a Multidisciplinary Requirements Development Approach.

PubMed

Van Velsen, Lex; Wentzel, Jobke; Van Gemert-Pijnen, Julia Ewc

2013-06-24

Requirements development is a crucial part of eHealth design. It entails all the activities devoted to requirements identification, the communication of requirements to other developers, and their evaluation. Currently, a requirements development approach geared towards the specifics of the eHealth domain is lacking. This is likely to result in a mismatch between the developed technology and end user characteristics, physical surroundings, and the organizational context of use. It also makes it hard to judge the quality of eHealth design, since it makes it difficult to gear evaluations of eHealth to the main goals it is supposed to serve. In order to facilitate the creation of eHealth that matters, we present a practical, multidisciplinary requirements development approach which is embedded in a holistic design approach for eHealth (the Center for eHealth Research roadmap) that incorporates both human-centered design and business modeling. Our requirements development approach consists of five phases. In the first, preparatory, phase the project team is composed and the overall goal(s) of the eHealth intervention are decided upon. Second, primary end users and other stakeholders are identified by means of audience segmentation techniques and our stakeholder identification method. Third, the designated context of use is mapped and end users are profiled by means of requirements elicitation methods (eg, interviews, focus groups, or observations). Fourth, stakeholder values and eHealth intervention requirements are distilled from data transcripts, which leads to phase five, in which requirements are communicated to other developers using a requirements notation template we developed specifically for the context of eHealth technologies. The end result of our requirements development approach for eHealth interventions is a design document which includes functional and non-functional requirements, a list of stakeholder values, and end user profiles in the form of personas (fictitious end users, representative of a primary end user group). The requirements development approach presented in this article enables eHealth developers to apply a systematic and multi-disciplinary approach towards the creation of requirements. The cooperation between health, engineering, and social sciences creates a situation in which a mismatch between design, end users, and the organizational context can be avoided. Furthermore, we suggest to evaluate eHealth on a feature-specific level in order to learn exactly why such a technology does or does not live up to its expectations.

The hetC Gene Is a Direct Target of the NtcA Transcriptional Regulator in Cyanobacterial Heterocyst Development

PubMed Central

Muro-Pastor, Alicia M.; Valladares, Ana; Flores, Enrique; Herrero, Antonia

1999-01-01

The heterocyst is the site of nitrogen fixation in aerobically grown cultures of some filamentous cyanobacteria. Heterocyst development in Anabaena sp. strain PCC 7120 is dependent on the global nitrogen regulator NtcA and requires, among others, the products of the hetR and hetC genes. Expression of hetC, tested by RNA- DNA hybridization, was impaired in an ntcA mutant. A nitrogen-regulated, NtcA-dependent putative transcription start point was localized at nucleotide −571 with respect to the hetC translational start. Sequences upstream from this transcription start point exhibit the structure of the canonical cyanobacterial promoter activated by NtcA, and purified NtcA protein specifically bound to a DNA fragment containing this promoter. Activation of expression of hetC during heterocyst development appears thus to be directly operated by NtcA. NtcA-mediated activation of hetR expression was not impaired in a hetC mutant, indicating that HetC is not an NtcA-dependent element required for hetR induction. PMID:10542167

Space Station Mission Planning Study (MPS) development study. Volume 3: Software development plan

NASA Technical Reports Server (NTRS)

Klus, W. L.

1987-01-01

A software development plan is presented for the definition, design, and implementation of the Space Station (SS) Payload Mission Planning System (MPS). This plan is an evolving document and must be updated periodically as the SS design and operations concepts as well as the SS MPS concept evolve. The major segments of this plan are as follows: an overview of the SS MPS and a description of its required capabilities including the computer programs identified as configurable items with an explanation of the place and function of each within the system; an overview of the project plan and a detailed description of each development project activity breaking each into lower level tasks where applicable; identification of the resources required and recommendations for the manner in which they should be utilized including recommended schedules and estimated manpower requirements; and a description of the practices, standards, and techniques recommended for the SS MPS Software (SW) development.

Quantitative analysis of relationships between irradiation parameters and the reproducibility of cyclotron-produced (99m)Tc yields.

PubMed

Tanguay, J; Hou, X; Buckley, K; Schaffer, P; Bénard, F; Ruth, T J; Celler, A

2015-05-21

Cyclotron production of (99m)Tc through the (100)Mo(p,2n) (99m)Tc reaction channel is actively being investigated as an alternative to reactor-based (99)Mo generation by nuclear fission of (235)U. An exciting aspect of this approach is that it can be implemented using currently-existing cyclotron infrastructure to supplement, or potentially replace, conventional (99m)Tc production methods that are based on aging and increasingly unreliable nuclear reactors. Successful implementation will require consistent production of large quantities of high-radionuclidic-purity (99m)Tc. However, variations in proton beam currents and the thickness and isotopic composition of enriched (100)Mo targets, in addition to other irradiation parameters, may degrade reproducibility of both radionuclidic purity and absolute (99m)Tc yields. The purpose of this article is to present a method for quantifying relationships between random variations in production parameters, including (100)Mo target thicknesses and proton beam currents, and reproducibility of absolute (99m)Tc yields (defined as the end of bombardment (EOB) (99m)Tc activity). Using the concepts of linear error propagation and the theory of stochastic point processes, we derive a mathematical expression that quantifies the influence of variations in various irradiation parameters on yield reproducibility, quantified in terms of the coefficient of variation of the EOB (99m)Tc activity. The utility of the developed formalism is demonstrated with an example. We show that achieving less than 20% variability in (99m)Tc yields will require highly-reproducible target thicknesses and proton currents. These results are related to the service rate which is defined as the percentage of (99m)Tc production runs that meet the minimum daily requirement of one (or many) nuclear medicine departments. For example, we show that achieving service rates of 84.0%, 97.5% and 99.9% with 20% variations in target thicknesses requires producing on average 1.2, 1.5 and 1.9 times the minimum daily activity requirement. The irradiation parameters that would be required to achieve these service rates are described. We believe the developed formalism will aid in the development of quality-control criteria required to ensure consistent supply of large quantities of high-radionuclidic-purity cyclotron-produced (99m)Tc.

Quantitative analysis of relationships between irradiation parameters and the reproducibility of cyclotron-produced 99mTc yields

NASA Astrophysics Data System (ADS)

Tanguay, J.; Hou, X.; Buckley, K.; Schaffer, P.; Bénard, F.; Ruth, T. J.; Celler, A.

2015-05-01

Cyclotron production of 99mTc through the 100Mo(p,2n) 99mTc reaction channel is actively being investigated as an alternative to reactor-based 99Mo generation by nuclear fission of 235U. An exciting aspect of this approach is that it can be implemented using currently-existing cyclotron infrastructure to supplement, or potentially replace, conventional 99mTc production methods that are based on aging and increasingly unreliable nuclear reactors. Successful implementation will require consistent production of large quantities of high-radionuclidic-purity 99mTc. However, variations in proton beam currents and the thickness and isotopic composition of enriched 100Mo targets, in addition to other irradiation parameters, may degrade reproducibility of both radionuclidic purity and absolute 99mTc yields. The purpose of this article is to present a method for quantifying relationships between random variations in production parameters, including 100Mo target thicknesses and proton beam currents, and reproducibility of absolute 99mTc yields (defined as the end of bombardment (EOB) 99mTc activity). Using the concepts of linear error propagation and the theory of stochastic point processes, we derive a mathematical expression that quantifies the influence of variations in various irradiation parameters on yield reproducibility, quantified in terms of the coefficient of variation of the EOB 99mTc activity. The utility of the developed formalism is demonstrated with an example. We show that achieving less than 20% variability in 99mTc yields will require highly-reproducible target thicknesses and proton currents. These results are related to the service rate which is defined as the percentage of 99mTc production runs that meet the minimum daily requirement of one (or many) nuclear medicine departments. For example, we show that achieving service rates of 84.0%, 97.5% and 99.9% with 20% variations in target thicknesses requires producing on average 1.2, 1.5 and 1.9 times the minimum daily activity requirement. The irradiation parameters that would be required to achieve these service rates are described. We believe the developed formalism will aid in the development of quality-control criteria required to ensure consistent supply of large quantities of high-radionuclidic-purity cyclotron-produced 99mTc.

Facilitating Behavior Change With Low-literacy Patient Education Materials

PubMed Central

Seligman, Hilary K.; Wallace, Andrea S.; DeWalt, Darren A.; Schillinger, Dean; Arnold, Connie L.; Shilliday, Betsy Bryant; Delgadillo, Adriana; Bengal, Nikki; Davis, Terry C.

2014-01-01

Objective To describe a process for developing low-literacy health education materials that increase knowledge and activate patients toward healthier behaviors. Methods We developed a theoretically informed process for developing educational materials. This process included convening a multidisciplinary creative team, soliciting stakeholder input, identifying key concepts to be communicated, mapping concepts to a behavioral theory, creating a supporting behavioral intervention, designing and refining materials, and assessing efficacy. Results We describe the use of this process to develop a diabetes self-management guide. Conclusions Developing low-literacy health education materials that will activate patients toward healthier behaviors requires attention to factors beyond reading level. PMID:17931139

Lobbying for international health: the link between good ideas and funded programs: Bread for the World and the Agency for International Development.

PubMed Central

Quinley, J C; Baker, T D

1986-01-01

Historically, the Agency for International Development (AIDS) health budget has been closely tied to overall development spending. A large increase in the international health appropriations in 1984 broke this pattern. Investigation shows that active grass roots organizing and congressional lobbying are the most likely responsible factors in the increase. Maintenance and expansion of this success will require increased recognition of and participation in these activities by individuals and organizations involved in international health. PMID:3717466

Software Development Cost Estimation Executive Summary

NASA Technical Reports Server (NTRS)

Hihn, Jairus M.; Menzies, Tim

2006-01-01

Identify simple fully validated cost models that provide estimation uncertainty with cost estimate. Based on COCOMO variable set. Use machine learning techniques to determine: a) Minimum number of cost drivers required for NASA domain based cost models; b) Minimum number of data records required and c) Estimation Uncertainty. Build a repository of software cost estimation information. Coordinating tool development and data collection with: a) Tasks funded by PA&E Cost Analysis; b) IV&V Effort Estimation Task and c) NASA SEPG activities.

Advanced composite elevator for Boeing 727 aircraft. Volume 1: Technical summary

NASA Technical Reports Server (NTRS)

Chovil, D. V.; Harvey, S. T.; Mccarty, J. E.; Desper, O. E.; Jamison, E. S.; Syder, H.

1981-01-01

The design, development, analysis, and testing activities and results that were required to produce five and one-half shipsets of advanced composite elevators for Boeing 727 aircraft are summarized. During the preliminary design period, alternative concepts were developed. After selection of the best design, detail design and basic configuration improvements were evaluated. Five and one-half shipsets were manufactured. All program goals (except competitive cost demonstration) were accomplished when our design met or exceeded all requirements, criteria, and objectives.

Combinatorial development of antibacterial Zr-Cu-Al-Ag thin film metallic glasses.

PubMed

Liu, Yanhui; Padmanabhan, Jagannath; Cheung, Bettina; Liu, Jingbei; Chen, Zheng; Scanley, B Ellen; Wesolowski, Donna; Pressley, Mariyah; Broadbridge, Christine C; Altman, Sidney; Schwarz, Udo D; Kyriakides, Themis R; Schroers, Jan

2016-05-27

Metallic alloys are normally composed of multiple constituent elements in order to achieve integration of a plurality of properties required in technological applications. However, conventional alloy development paradigm, by sequential trial-and-error approach, requires completely unrelated strategies to optimize compositions out of a vast phase space, making alloy development time consuming and labor intensive. Here, we challenge the conventional paradigm by proposing a combinatorial strategy that enables parallel screening of a multitude of alloys. Utilizing a typical metallic glass forming alloy system Zr-Cu-Al-Ag as an example, we demonstrate how glass formation and antibacterial activity, two unrelated properties, can be simultaneously characterized and the optimal composition can be efficiently identified. We found that in the Zr-Cu-Al-Ag alloy system fully glassy phase can be obtained in a wide compositional range by co-sputtering, and antibacterial activity is strongly dependent on alloy compositions. Our results indicate that antibacterial activity is sensitive to Cu and Ag while essentially remains unchanged within a wide range of Zr and Al. The proposed strategy not only facilitates development of high-performing alloys, but also provides a tool to unveil the composition dependence of properties in a highly parallel fashion, which helps the development of new materials by design.

Combinatorial development of antibacterial Zr-Cu-Al-Ag thin film metallic glasses

NASA Astrophysics Data System (ADS)

Liu, Yanhui; Padmanabhan, Jagannath; Cheung, Bettina; Liu, Jingbei; Chen, Zheng; Scanley, B. Ellen; Wesolowski, Donna; Pressley, Mariyah; Broadbridge, Christine C.; Altman, Sidney; Schwarz, Udo D.; Kyriakides, Themis R.; Schroers, Jan

2016-05-01

Metallic alloys are normally composed of multiple constituent elements in order to achieve integration of a plurality of properties required in technological applications. However, conventional alloy development paradigm, by sequential trial-and-error approach, requires completely unrelated strategies to optimize compositions out of a vast phase space, making alloy development time consuming and labor intensive. Here, we challenge the conventional paradigm by proposing a combinatorial strategy that enables parallel screening of a multitude of alloys. Utilizing a typical metallic glass forming alloy system Zr-Cu-Al-Ag as an example, we demonstrate how glass formation and antibacterial activity, two unrelated properties, can be simultaneously characterized and the optimal composition can be efficiently identified. We found that in the Zr-Cu-Al-Ag alloy system fully glassy phase can be obtained in a wide compositional range by co-sputtering, and antibacterial activity is strongly dependent on alloy compositions. Our results indicate that antibacterial activity is sensitive to Cu and Ag while essentially remains unchanged within a wide range of Zr and Al. The proposed strategy not only facilitates development of high-performing alloys, but also provides a tool to unveil the composition dependence of properties in a highly parallel fashion, which helps the development of new materials by design.

Combinatorial development of antibacterial Zr-Cu-Al-Ag thin film metallic glasses

PubMed Central

Liu, Yanhui; Padmanabhan, Jagannath; Cheung, Bettina; Liu, Jingbei; Chen, Zheng; Scanley, B. Ellen; Wesolowski, Donna; Pressley, Mariyah; Broadbridge, Christine C.; Altman, Sidney; Schwarz, Udo D.; Kyriakides, Themis R.; Schroers, Jan

2016-01-01

Metallic alloys are normally composed of multiple constituent elements in order to achieve integration of a plurality of properties required in technological applications. However, conventional alloy development paradigm, by sequential trial-and-error approach, requires completely unrelated strategies to optimize compositions out of a vast phase space, making alloy development time consuming and labor intensive. Here, we challenge the conventional paradigm by proposing a combinatorial strategy that enables parallel screening of a multitude of alloys. Utilizing a typical metallic glass forming alloy system Zr-Cu-Al-Ag as an example, we demonstrate how glass formation and antibacterial activity, two unrelated properties, can be simultaneously characterized and the optimal composition can be efficiently identified. We found that in the Zr-Cu-Al-Ag alloy system fully glassy phase can be obtained in a wide compositional range by co-sputtering, and antibacterial activity is strongly dependent on alloy compositions. Our results indicate that antibacterial activity is sensitive to Cu and Ag while essentially remains unchanged within a wide range of Zr and Al. The proposed strategy not only facilitates development of high-performing alloys, but also provides a tool to unveil the composition dependence of properties in a highly parallel fashion, which helps the development of new materials by design. PMID:27230692

Retinoid X receptor and peroxisome proliferator-activated receptor activate an estrogen responsive gene independent of the estrogen receptor.

PubMed

Nuñez, S B; Medin, J A; Braissant, O; Kemp, L; Wahli, W; Ozato, K; Segars, J H

1997-03-14

Estrogen receptors regulate transcription of genes essential for sexual development and reproductive function. Since the retinoid X receptor (RXR) is able to modulate estrogen responsive genes and both 9-cis RA and fatty acids influenced development of estrogen responsive tumors, we hypothesized that estrogen responsive genes might be modulated by RXR and the fatty acid receptor (peroxisome proliferator-activated receptor, PPAR). To test this hypothesis, transfection assays in CV-1 cells were performed with an estrogen response element (ERE) coupled to a luciferase reporter construct. Addition of expression vectors for RXR and PPAR resulted in an 11-fold increase in luciferase activity in the presence of 9-cis RA. Furthermore, mobility shift assays demonstrated binding of RXR and PPAR to the vitellogenin A2-ERE and an ERE in the oxytocin promoter. Methylation interference assays demonstrated that specific guanine residues required for RXR/PPAR binding to the ERE were similar to residues required for ER binding. Moreover, RXR domain-deleted constructs in transfection assays showed that activation required RXR since an RXR delta AF-2 mutant completely abrogated reporter activity. Oligoprecipitation binding studies with biotinylated ERE and (35)S-labeled in vitro translated RXR constructs confirmed binding of delta AF-2 RXR mutant to the ERE in the presence of baculovirus-expressed PPAR. Finally, in situ hybridization confirmed RXR and PPAR mRNA expression in estrogen responsive tissues. Collectively, these data suggest that RXR and PPAR are present in reproductive tissues, are capable of activating estrogen responsive genes and suggest that the mechanism of activation may involve direct binding of the receptors to estrogen response elements.

34 CFR 350.62 - What are the matching requirements?

Code of Federal Regulations, 2010 CFR

2010-07-01

... of vocational and other rehabilitation services, and research training and career development... Engineering Research Centers; and (ii) Specialized research or demonstration activities described in section...

24 CFR 92.352 - Environmental review.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Environmental review. 92.352... Development HOME INVESTMENT PARTNERSHIPS PROGRAM Other Federal Requirements § 92.352 Environmental review. (a) General. The environmental effects of each activity carried out with HOME funds must be assessed in...

24 CFR 92.352 - Environmental review.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Environmental review. 92.352... Development HOME INVESTMENT PARTNERSHIPS PROGRAM Other Federal Requirements § 92.352 Environmental review. (a) General. The environmental effects of each activity carried out with HOME funds must be assessed in...

44 CFR 208.23 - Allowable costs under Preparedness Cooperative Agreements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... as personnel compensation and benefits relating to System maintenance and development, record keeping, inventory of equipment, and correspondence; (2) Travel to and from System activities, meetings, conferences... DHS requires to meet its standards. (b) Training: (1) Development and delivery of, and participation...

Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing.

PubMed

Cao, Zhenbo; Bulleid, Neil J

The activity of typical 2-cys peroxiredoxin (Prxs) can be regulated by hyperoxidation with a consequent loss of redox activity. Here we developed a simple assay to monitor the level of hyperoxidation of different typical 2-cys prxs simultaneously. This assay only requires standard equipment and can compare different samples on the same gel. It requires much less time than conventional 2D gels and gives more information than Western blotting with an antibody specific for hyperoxidized peroxiredoxin. This method could also be used to monitor protein modification with a charge difference such as phosphorylation.

WFIRST Project Science Activities

NASA Technical Reports Server (NTRS)

Gehrels, Neil

2012-01-01

The WFIRST Project is a joint effort between GSFC and JPL. The project scientists and engineers are working with the community Science Definition Team to define the requirements and initial design of the mission. The objective is to design an observatory that meets the WFIRST science goals of the Astr02010 Decadal Survey for minimum cost. This talk will be a report of recent project activities including requirements flowdown, detector array development, science simulations, mission costing and science outreach. Details of the interim mission design relevant to scientific capabilities will be presented.

Botrytis cinerea Manipulates the Antagonistic Effects between Immune Pathways to Promote Disease Development in Tomato[C][W][OA

PubMed Central

El Oirdi, Mohamed; El Rahman, Taha Abd; Rigano, Luciano; El Hadrami, Abdelbasset; Rodriguez, María Cecilia; Daayf, Fouad; Vojnov, Adrian; Bouarab, Kamal

2011-01-01

Plants have evolved sophisticated mechanisms to sense and respond to pathogen attacks. Resistance against necrotrophic pathogens generally requires the activation of the jasmonic acid (JA) signaling pathway, whereas the salicylic acid (SA) signaling pathway is mainly activated against biotrophic pathogens. SA can antagonize JA signaling and vice versa. Here, we report that the necrotrophic pathogen Botrytis cinerea exploits this antagonism as a strategy to cause disease development. We show that B. cinerea produces an exopolysaccharide, which acts as an elicitor of the SA pathway. In turn, the SA pathway antagonizes the JA signaling pathway, thereby allowing the fungus to develop its disease in tomato (Solanum lycopersicum). SA-promoted disease development occurs through Nonexpressed Pathogen Related1. We also show that the JA signaling pathway required for tomato resistance against B. cinerea is mediated by the systemin elicitor. These data highlight a new strategy used by B. cinerea to overcome the plant’s defense system and to spread within the host. PMID:21665999

The Changing Workplace: Implications of Quality of Work Life Developments for Vocational Education. Research and Development Series No. 249.

ERIC Educational Resources Information Center

Pratzner, Frank C.; Russell, Jill Frymier

Based upon a review of literature and on-site interviews and observations at nine firms that are recognized leaders in the development and implementation of quality of work life (QWL) activities, this report examines implications of QWL developments for future skill requirements and their potential consequences for public vocational education…

EVA Training and Development Facilities

NASA Technical Reports Server (NTRS)

Cupples, Scott

2016-01-01

Overview: Vast majority of US EVA (ExtraVehicular Activity) training and EVA hardware development occurs at JSC; EVA training facilities used to develop and refine procedures and improve skills; EVA hardware development facilities test hardware to evaluate performance and certify requirement compliance; Environmental chambers enable testing of hardware from as large as suits to as small as individual components in thermal vacuum conditions.

Communication Problems in Requirements Engineering: A Field Study

NASA Technical Reports Server (NTRS)

Al-Rawas, Amer; Easterbrook, Steve

1996-01-01

The requirements engineering phase of software development projects is characterized by the intensity and importance of communication activities. During this phase, the various stakeholders must be able to communicate their requirements to the analysts, and the analysts need to be able to communicate the specifications they generate back to the stakeholders for validation. This paper describes a field investigation into the problems of communication between disparate communities involved in the requirements specification activities. The results of this study are discussed in terms of their relation to three major communication barriers: (1) ineffectiveness of the current communication channels; (2) restrictions on expressiveness imposed by notations; and (3) social and organizational barriers. The results confirm that organizational and social issues have great influence on the effectiveness of communication. They also show that in general, end-users find the notations used by software practitioners to model their requirements difficult to understand and validate.

IL-32γ promotes integrin αvβ6 expression through the activation of NF-κB in HSCs

PubMed Central

Liu, Hongcan; Pan, Xingfei; Cao, Hong; Shu, Xin; Sun, Haixia; Lu, Jianxi; Liang, Jiayin; Zhang, Ka; Zhu, Fengqin; Li, Gang; Zhang, Qi

2017-01-01

Hepatic stellate cell (HSC) activation is important in the pathogenesis of liver fibrosis. However, the molecular mechanism of HSC activation is not completely understood. In the present study, it was demonstrated that interleukin-32γ (IL-32γ) is capable of enhancing intefgrin αvβ6 expression by inducing integrin αvβ6 promoter activity in a dose-dependent manner in HSCs. Furthermore, it was determined that nuclear factor κB (NF-κB) activation is required for IL-32γ-induced integrin αvβ6 expression. Increased integrin αvβ6 expression is then able to activate HSCs. These results indicate that NF-κB activation is required for IL-32γ to induce integrin αvβ6 expression and consequently promote HSC activation. Therefore, IL-32γ activates HSCs and therefore may be associated with hepatic fibrogenesis. These results may enable the development of novel effective strategies to treat hepatic fibrosis. PMID:29042996

TRPV3 channels mediate strontium-induced mouse egg activation

PubMed Central

Carvacho, Ingrid; Lee, Hoi Chang; Fissore, Rafael A.; Clapham, David E.

2014-01-01

SUMMARY In mammals, calcium influx is required for oocyte maturation and egg activation. The molecular identities of the calcium-permeant channels that underlie the initiation of embryonic development are not established. Here, we describe a Transient Receptor Potential (TRP) ion channel current activated by TRP agonists that is absent in TrpV3−/− eggs. TRPV3 current is differentially expressed during oocyte maturation, reaching a peak of maximum density and activity at metaphase of meiosis II (MII), the stage of fertilization. Selective activation of TRPV3 channels provokes egg activation by mediating massive calcium entry. Widely used to activate eggs, strontium application is known to yield normal offspring in combination with somatic cell nuclear transfer. We show that TRPV3 is required for strontium influx, as TrpV3−/− eggs failed to permeate Sr2+ or undergo strontium-induced activation. We propose that TRPV3 is the major mediator of calcium influx in mouse eggs and is a putative target for artificial egg activation. PMID:24316078

Engineering aspects of geothermal development with emphasis on the Imperial Valley of California

NASA Technical Reports Server (NTRS)

Goldsmith, M.

1978-01-01

This review was prepared in support of a geothermal planning activity of the County of Imperial. Engineering features of potential geothermal development are outlined. Acreage requirements for drilling and powerplants are estimated, as are the costs for wells, fluid transmission pipes, and generating stations. Rough scaling relationships are developed for cost factors as a function of reservoir temperature. Estimates are made for cooling water requirements, and possible sources of cooling water are discussed. Availability and suitability of agricultural wastewater for cooling are emphasized. The utility of geothermal resources for fresh water production in the Imperial Valley is considered.

Study of the application of advanced technologies to long-range transport aircraft. Volume 2: Research and development requirements

NASA Technical Reports Server (NTRS)

Lange, R. H.; Sturgeon, R. F.; Adams, W. E.; Bradley, E. S.; Cahill, J. F.; Eudaily, R. R.; Hancock, J. P.; Moore, J. W.

1972-01-01

Investigations were conducted to evaluate the relative benefits attainable through the exploitation of advanced technologies and to identify future research and development efforts required to permit the application of selected technologies to transport aircraft entering commercial operation in 1985. Results show that technology advances, particularly in the areas of composite materials, supercritical aerodynamics, and active control systems, will permit the development of long-range, high-payload commercial transports operating at high-subsonic speeds with direct operating costs lower than those of current aircraft. These advanced transports also achieve lower noise levels and lower engine pollutant emissions than current transports. Research and development efforts, including analytical investigations, laboratory test programs, and flight test programs, are required in essentially all technology areas to achieve the potential technology benefits.

Development of an expert system prototype for determining software functional requirements for command management activities at NASA Goddard

NASA Technical Reports Server (NTRS)

Liebowitz, J.

1986-01-01

The development of an expert system prototype for software functional requirement determination for NASA Goddard's Command Management System, as part of its process of transforming general requests into specific near-earth satellite commands, is described. The present knowledge base was formulated through interactions with domain experts, and was then linked to the existing Knowledge Engineering Systems (KES) expert system application generator. Steps in the knowledge-base development include problem-oriented attribute hierarchy development, knowledge management approach determination, and knowledge base encoding. The KES Parser and Inspector, in addition to backcasting and analogical mapping, were used to validate the expert system-derived requirements for one of the major functions of a spacecraft, the solar Maximum Mission. Knowledge refinement, evaluation, and implementation procedures of the expert system were then accomplished.

Segment-specific expression of alkaline phosphatase in the Tubifex embryo requires DNA replication and mRNA synthesis.

PubMed

Kitamura, Kaoru; Shimizu, Takashi

2002-04-15

During embryogenesis of the oligochaete annelid Tubifex, segments VII and VIII specifically express mesodermal alkaline phosphatase (ALP) activity in the ventrolateral region. In this study, using specific inhibitors, we examined whether this segment-specific expression of ALP activity depends on DNA replication and RNA transcription. BrdU-incorporation experiments showed that presumptive ALP-expressing cells undergo the last round of DNA replication at 12-24 hr prior to emergence of ALP activity. When this DNA replication was inhibited by aphidicolin, ALP development was completely abrogated in the ventrolateral mesoderm. Similar inhibition of ALP development was also observed in alpha-amanitin-injected embryos. While injection of alpha-amanitin at 24 hr prior to the emergence of ALP activity exerted inhibitory effects on ALP development, injection at 14 hr was no longer effective. In contrast, ALP activity developed normally in cytochalasin-D-treated embryos in which cytokinesis was prevented from occurring for 36 hs prior to appearance of ALP activity. These results suggest that the segment-specific development of ALP activity in the Tubifex embryo depends on DNA replication and mRNA transcription, both of which occur long before the emergence of ALP activity. Copyright 2002 Wiley-Liss, Inc.

Towards a rapid and comprehensive microbial detection and identification system for life support and planetary protection applications

NASA Astrophysics Data System (ADS)

Lasseur, Christophe

Long term manned missions of our Russian colleagues have demonstrated the risks associated with microbial contamination. These risks concern both crew health via the metabolic consumables contamination (water, air,.) but and also the hardware degradation. In parallel to these life support issues, planetary protection experts have agreed to place clear specifications of the microbial quality of future hardware landing on extraterrestrial planets as well as elaborate the requirements of contamination for manned missions on surface. For these activities, it is necessary to have a better understanding of microbial activity, to create culture collections and to develop on-line detection tools. . In this respect, over the last 6 years , ESA has supported active scientific research on the choice of critical genes and functions, including those linked to horizontal gene pool of bacteria and its dissemination. In parallel, ESA and European industries have been developing an automated instrument for rapid microbial detection on air and surface samples. Within this paper, we first present the life support and planetary protection requirements, and the state of the art of the instrument development. Preliminary results at breadboard level, including a mock-up view of the final instrument are also presented. Finally, the remaining steps required to reach a functional instrument for planetary hardware integration and life support flight hardware are also presented.

Advanced automation for in-space vehicle processing

NASA Technical Reports Server (NTRS)

Sklar, Michael; Wegerif, D.

1990-01-01

The primary objective of this 3-year planned study is to assure that the fully evolved Space Station Freedom (SSF) can support automated processing of exploratory mission vehicles. Current study assessments show that required extravehicular activity (EVA) and to some extent intravehicular activity (IVA) manpower requirements for required processing tasks far exceeds the available manpower. Furthermore, many processing tasks are either hazardous operations or they exceed EVA capability. Thus, automation is essential for SSF transportation node functionality. Here, advanced automation represents the replacement of human performed tasks beyond the planned baseline automated tasks. Both physical tasks such as manipulation, assembly and actuation, and cognitive tasks such as visual inspection, monitoring and diagnosis, and task planning are considered. During this first year of activity both the Phobos/Gateway Mars Expedition and Lunar Evolution missions proposed by the Office of Exploration have been evaluated. A methodology for choosing optimal tasks to be automated has been developed. Processing tasks for both missions have been ranked on the basis of automation potential. The underlying concept in evaluating and describing processing tasks has been the use of a common set of 'Primitive' task descriptions. Primitive or standard tasks have been developed both for manual or crew processing and automated machine processing.

Solar Cell and Array Technology Development for NASA Solar Electric Propulsion Missions

NASA Technical Reports Server (NTRS)

Piszczor, Michael; McNatt, Jeremiah; Mercer, Carolyn; Kerslake, Tom; Pappa, Richard

2012-01-01

NASA is currently developing advanced solar cell and solar array technologies to support future exploration activities. These advanced photovoltaic technology development efforts are needed to enable very large (multi-hundred kilowatt) power systems that must be compatible with solar electric propulsion (SEP) missions. The technology being developed must address a wide variety of requirements and cover the necessary advances in solar cell, blanket integration, and large solar array structures that are needed for this class of missions. Th is paper will summarize NASA's plans for high power SEP missions, initi al mission studies and power system requirements, plans for advanced photovoltaic technology development, and the status of specific cell and array technology development and testing that have already been conducted.

Proceedings of the Introducing Requirements Management into Organizations Workshop: Requirements Management Transition Packages November 11-13, 1996.

DTIC Science & Technology

1997-04-25

the community, as with the Systems Engineering Capability Maturity Model ? • Should it be built by a small working group ? • What role should... collaborating on the development of the transition package concept for requirements management with two organizations. When participants in the workshop...34 learning " and approaches it as a qualitative activity among qualified peers [Spendolini 92]. In the spirit of this style of

24 CFR 585.305 - Eligible activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... payments and other assistance required to comply with § 585.308, legal fees, and construction management...) Wages, benefits and need-based stipends provided to participants; (j) Leadership development, counseling...

Tritium technology development in EEC laboratories contributions to design goals for NET

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dinner, P.; Chazalon, M.; Leger, D.

1988-09-01

An overview is given of the tritium technology activities carried out in the European national laboratories associated with the European Fusion Programme and in the European Joint Research Center. The relationship of these activities to the Next European Torus (NET) design priorities is discussed, and the current status of the research is summarised. Future developments, required for NET, which will be addressed in the definition of the next 5-year programme are also presented.

Towards Requirements in Systems Engineering for Aerospace IVHM Design

NASA Technical Reports Server (NTRS)

Saxena, Abhinav; Roychoudhury, Indranil; Lin, Wei; Goebel, Kai

2013-01-01

Health management (HM) technologies have been employed for safety critical system for decades, but a coherent systematic process to integrate HM into the system design is not yet clear. Consequently, in most cases, health management resorts to be an after-thought or 'band-aid' solution. Moreover, limited guidance exists for carrying out systems engineering (SE) on the subject of writing requirements for designs with integrated vehicle health management (IVHM). It is well accepted that requirements are key to developing a successful IVHM system right from the concept stage to development, verification, utilization, and support. However, writing requirements for systems with IVHM capability have unique challenges that require the designers to look beyond their own domains and consider the constraints and specifications of other interlinked systems. In this paper we look at various stages in the SE process and identify activities specific to IVHM design and development. More importantly, several relevant questions are posed that system engineers must address at various design and development stages. Addressing these questions should provide some guidance to systems engineers towards writing IVHM related requirements to ensure that appropriate IVHM functions are built into the system design.

ROCK and RHO Playlist for Preimplantation Development: Streaming to HIPPO Pathway and Apicobasal Polarity in the First Cell Differentiation.

PubMed

Alarcon, Vernadeth B; Marikawa, Yusuke

2018-01-01

In placental mammalian development, the first cell differentiation produces two distinct lineages that emerge according to their position within the embryo: the trophectoderm (TE, placenta precursor) differentiates in the surface, while the inner cell mass (ICM, fetal body precursor) forms inside. Here, we discuss how such position-dependent lineage specifications are regulated by the RHOA subfamily of small GTPases and RHO-associated coiled-coil kinases (ROCK). Recent studies in mouse show that activities of RHO/ROCK are required to promote TE differentiation and to concomitantly suppress ICM formation. RHO/ROCK operate through the HIPPO signaling pathway, whose cell position-specific modulation is central to establishing unique gene expression profiles that confer cell fate. In particular, activities of RHO/ROCK are essential in outside cells to promote nuclear localization of transcriptional co-activators YAP/TAZ, the downstream effectors of HIPPO signaling. Nuclear localization of YAP/TAZ depends on the formation of apicobasal polarity in outside cells, which requires activities of RHO/ROCK. We propose models of how RHO/ROCK regulate lineage specification and lay out challenges for future investigations to deepen our understanding of the roles of RHO/ROCK in preimplantation development. Finally, as RHO/ROCK may be inhibited by certain pharmacological agents, we discuss their potential impact on human preimplantation development in relation to fertility preservation in women.

Intra-Extra Vehicular Activity Apollo Spacesuits

NASA Technical Reports Server (NTRS)

Thomas, Kenneth S.

2016-01-01

Kenneth Thomas will discuss the Apollo Intra-Extra Vehicular Activity (IEVA) spacesuits, which supported launch and reentry and extra-vehicular activity. This program was NASA's first attempt to develop a new suit design from requirements and concepts. Mr. Thomas will chronicle the challenges, developments, struggles, and solutions that culminated in the system that allowed the first human exploration of the Moon and deep space (outside low-Earth orbit). Apollo pressure suit designs allowed the heroic repair of the Skylab space station and supported the first U.S. and Russian spacecraft docking during the Apollo Soyuz Test Project. Mr. Thomas will also discuss the IEVA suits' successes and challenges associated with the IEVA developments of the 1960s.

Transforming Functional Requirements from UML into BPEL to Efficiently Develop SOA-Based Systems

NASA Astrophysics Data System (ADS)

Vemulapalli, Anisha; Subramanian, Nary

The intended behavior of any system such as services, tasks or functions can be captured by functional requirements of the system. As our dependence on online services has grown steadily, the web applications are being developed employing the SOA. BPEL4WS provides a means for expressing functional requirements of an SOA-based system by providing constructs to capture business goals and objectives for the system. In this paper we propose an approach for transforming user-centered requirements captured using UML into a corresponding BPEL specification, where the business processes are captured by means of use-cases from which UML sequence diagrams and activity diagrams are extracted. Subsequently these UML models are mapped to BPEL specifications that capture the essence of the initial business requirements to develop the SOA-based system by employing CASE tools. A student housing system is used as a case study to illustrate this approach and the system is validated using NetBeans.

How to improve the promotion of Korean beef barbecue, bulgogi, for international customers. An application of quality function deployment.

PubMed

Park, So-Hyun; Ham, Sunny; Lee, Min-A

2012-10-01

Quality function deployment (QFD) is a product development technique that translates customer requirements into activities for the development of products and services. This study utilizes QFD to identify American customer's requirements for bulgogi, a popular Korean dish among international customers, and how to fulfill those requirements. A customer survey and an expert opinion survey were conducted for US customers. The top five customer requirements for bulgogi were identified as taste, freshness, flavor, tenderness, and juiciness; ease of purchase was included in the place of tenderness after calculating the weight requirements. Eighteen engineering characteristics were developed, and a 'localization of bulgogi menu' is strongly related to the other characteristics as well. The results from the calculation of relative importance of engineering characteristics identified that the 'control of marinating time', 'localization of bulgogi menu', 'improvement of cooking and serving process', 'development of recipe by parts of beef', and 'use of various seasonings' were the highest contributors to the overall improvement of bulgogi. The relative importance of engineering characteristics, correlation, and technical difficulties are ranked and integrated to develop the most effective strategy. The findings are discussed relative to industry implications. Copyright © 2012 Elsevier Ltd. All rights reserved.

From Amorphous to Defined: Balancing the Risks of Spiral Development

DTIC Science & Technology

2007-04-30

630 675 720 765 810 855 900 Time (Week) Work started and active PhIt [Requirements,Iter1] : JavelinCalibration work packages1 1 1 Work started and...active PhIt [Technology,Iter1] : JavelinCalibration work packages2 2 2 Work started and active PhIt [Design,Iter1] : JavelinCalibration work packages3 3 3 3...Work started and active PhIt [Manufacturing,Iter1] : JavelinCalibration work packages4 4 Work started and active PhIt [Use,Iter1] : JavelinCalibration

From the greenhouse to the field: Cultivation requirements of Arizona cliffrose (Purshia subintegra)

Treesearch

Joanne E. Baggs; Joyce Maschinski

2001-01-01

Central to the conservation of the federally endangered Purshia subintegra (Arizona cliffrose) is development of an understanding of its cultivation requirements. This knowledge will enable us to augment declining or threatened populations as well as preserve genotypes impacted by human activities. We studied seed and stem cutting propagation of P. subintegra. The...

MANUAL OF PROCEDURES AND CRITERIA FOR CAMPUS DEVELOPMENT AND CAPITAL OUTLAY PLANNING.

ERIC Educational Resources Information Center

HELDMAN, HERBERT; MASON, THOMAS R.

THE PURPOSE OF THIS MANUAL IS TO PROVIDE A BASIS FOR SYSTEMATICALLY PROGRAMMING THE REQUIREMENTS FOR PHYSICAL PLANT AND LAND TO ACCOMMODATE THE NEEDS OF AN INSTITUTION UNDER A SPECIFIED SET OF CIRCUMSTANCES. THE MANUAL IS ORGANIZED INTO SIX BROAD ELEMENTS--(1) ACTIVITY LEVELS OF ENROLLMENT, INSTRUCTIONAL WORKLOADS AND STAFF REQUIREMENTS, (2)…

13 CFR 120.828 - Minimum level of 504 loan activity and restrictions on portfolio concentrations.

Code of Federal Regulations, 2010 CFR

2010-01-01

... SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Requirements for Cdc... concentrations. (a) A CDC is required to receive SBA approval of at least four 504 loan approvals during two consecutive fiscal years. (b) A CDC's 504 loan portfolio must be diversified by business sector. [68 FR 57981...

13 CFR 120.828 - Minimum level of 504 loan activity and restrictions on portfolio concentrations.

Code of Federal Regulations, 2014 CFR

2014-01-01

... SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Requirements for Cdc... concentrations. (a) A CDC is required to receive SBA approval of at least four 504 loan approvals during two consecutive fiscal years. (b) A CDC's 504 loan portfolio must be diversified by business sector. [68 FR 57981...

13 CFR 120.828 - Minimum level of 504 loan activity and restrictions on portfolio concentrations.

Code of Federal Regulations, 2011 CFR

2011-01-01

... SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Requirements for Cdc... concentrations. (a) A CDC is required to receive SBA approval of at least four 504 loan approvals during two consecutive fiscal years. (b) A CDC's 504 loan portfolio must be diversified by business sector. [68 FR 57981...

13 CFR 120.828 - Minimum level of 504 loan activity and restrictions on portfolio concentrations.

Code of Federal Regulations, 2013 CFR

2013-01-01

... SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Requirements for Cdc... concentrations. (a) A CDC is required to receive SBA approval of at least four 504 loan approvals during two consecutive fiscal years. (b) A CDC's 504 loan portfolio must be diversified by business sector. [68 FR 57981...

13 CFR 120.828 - Minimum level of 504 loan activity and restrictions on portfolio concentrations.

Code of Federal Regulations, 2012 CFR

2012-01-01

... SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Requirements for Cdc... concentrations. (a) A CDC is required to receive SBA approval of at least four 504 loan approvals during two consecutive fiscal years. (b) A CDC's 504 loan portfolio must be diversified by business sector. [68 FR 57981...

Tafenoquine and NPC-1161B require CYP 2D metabolism for anti-malarial activity: implications for the 8-aminoquinoline class of anti-malarial compounds

PubMed Central

2014-01-01

Background Tafenoquine (TQ) is an 8-aminoquinoline (8AQ) that has been tested in several Phase II and Phase III clinical studies and is currently in late stage development as an anti-malarial prophylactic agent. NPC-1161B is a promising 8AQ in late preclinical development. It has recently been reported that the 8AQ drug primaquine requires metabolic activation by CYP 2D6 for efficacy in humans and in mice, highlighting the importance of pharmacogenomics in the target population when administering primaquine. A logical follow-up study was to determine whether CYP 2D activation is required for other compounds in the 8AQ structural class. Methods In the present study, the anti-malarial activities of NPC-1161B and TQ were assessed against luciferase expressing Plasmodium berghei in CYP 2D knock-out mice in comparison with normal C57BL/6 mice (WT) and with humanized/CYP 2D6 knock-in mice by monitoring luminescence with an in vivo imaging system. These experiments were designed to determine the direct effects of CYP 2D metabolic activation on the anti-malarial efficacy of NPC-1161B and TQ. Results NPC-1161B and TQ exhibited no anti-malarial activity in CYP 2D knock-out mice when dosed at their ED100 values (1 mg/kg and 3 mg/kg, respectively) established in WT mice. TQ anti-malarial activity was partially restored in humanized/CYP 2D6 knock-in mice when tested at two times its ED100. Conclusions The results reported here strongly suggest that metabolism of NPC-1161B and TQ by the CYP 2D enzyme class is essential for their anti-malarial activity. Furthermore, these results may provide a possible explanation for therapeutic failures for patients who do not respond to 8AQ treatment for relapsing malaria. Because CYP 2D6 is highly polymorphic, variable expression of this enzyme in humans represents a significant pharmacogenomic liability for 8AQs which require CYP 2D metabolic activation for efficacy, particularly for large-scale prophylaxis and eradication campaigns. PMID:24386891

Tafenoquine and NPC-1161B require CYP 2D metabolism for anti-malarial activity: implications for the 8-aminoquinoline class of anti-malarial compounds.

PubMed

Marcsisin, Sean R; Sousa, Jason C; Reichard, Gregory A; Caridha, Diana; Zeng, Qiang; Roncal, Norma; McNulty, Ronan; Careagabarja, Julio; Sciotti, Richard J; Bennett, Jason W; Zottig, Victor E; Deye, Gregory; Li, Qigui; Read, Lisa; Hickman, Mark; Dhammika Nanayakkara, N P; Walker, Larry A; Smith, Bryan; Melendez, Victor; Pybus, Brandon S

2014-01-03

Tafenoquine (TQ) is an 8-aminoquinoline (8AQ) that has been tested in several Phase II and Phase III clinical studies and is currently in late stage development as an anti-malarial prophylactic agent. NPC-1161B is a promising 8AQ in late preclinical development. It has recently been reported that the 8AQ drug primaquine requires metabolic activation by CYP 2D6 for efficacy in humans and in mice, highlighting the importance of pharmacogenomics in the target population when administering primaquine. A logical follow-up study was to determine whether CYP 2D activation is required for other compounds in the 8AQ structural class. In the present study, the anti-malarial activities of NPC-1161B and TQ were assessed against luciferase expressing Plasmodium berghei in CYP 2D knock-out mice in comparison with normal C57BL/6 mice (WT) and with humanized/CYP 2D6 knock-in mice by monitoring luminescence with an in vivo imaging system. These experiments were designed to determine the direct effects of CYP 2D metabolic activation on the anti-malarial efficacy of NPC-1161B and TQ. NPC-1161B and TQ exhibited no anti-malarial activity in CYP 2D knock-out mice when dosed at their ED100 values (1 mg/kg and 3 mg/kg, respectively) established in WT mice. TQ anti-malarial activity was partially restored in humanized/CYP 2D6 knock-in mice when tested at two times its ED100. The results reported here strongly suggest that metabolism of NPC-1161B and TQ by the CYP 2D enzyme class is essential for their anti-malarial activity. Furthermore, these results may provide a possible explanation for therapeutic failures for patients who do not respond to 8AQ treatment for relapsing malaria. Because CYP 2D6 is highly polymorphic, variable expression of this enzyme in humans represents a significant pharmacogenomic liability for 8AQs which require CYP 2D metabolic activation for efficacy, particularly for large-scale prophylaxis and eradication campaigns.

Reverse Induced Fit-Driven MAS-Downstream Transduction: Looking for Metabotropic Agonists.

PubMed

Pernomian, Larissa; Gomes, Mayara S; de Paula da Silva, Carlos H Tomich; Rosa, Joaquin M C

2017-01-01

Protective effects of MAS activation have spurred clinical interests in developing MAS agonists. However, current bases that drive this process preclude that physiological concentrations of peptide MAS agonists induce an atypical signaling that does not reach the metabotropic efficacy of constitutive activation. Canonical activation of MAS-coupled G proteins is only achieved by supraphysiological concentrations of peptide MAS agonists or physiological concentrations of chemically modified analogues. These pleiotropic differences are because of two overlapped binding domains: one non-metabotropic site that recognizes peptide agonists and one metabotropic domain that recognizes modified analogues. It is feasible that supraphysiological concentrations of peptide MAS agonists undergo to chemical modifications required for binding to metabotropic domain. Receptor oligomerization enhances pharmacological parameters coupled to metabotropic signaling. The formation of receptor-signalosome complex makes the transduction of agonists more adaptive. Considering the recent identification of MAS-signalosome, we aimed to postulate the reverse induced fit hypothesis in which MAS-signalosome would trigger chemical modifications required for agonists bind to MAS metabotropic domain. Here we cover rational perspectives for developing novel metabotropic MAS agonists in the view of the reverse induced-fit hypothesis. Predicting a 3D model of MAS metabotropic domain may guide the screening of chemical modifications required for metabotropic efficacy. Pharmacophore-based virtual screening would select potential metabotropic MAS agonists from virtual libraries from human proteome. Rational perspectives that consider reverse induced fit hypothesis during MAS activation for developing metabotropic MAS agonists represents the best approach in providing MAS ligands with constitutive efficacy at physiological concentrations. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Generic health/safety/environment cases

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kelland, A.N.; Primrose, M.; Pickles, J.C.

1996-12-31

A desire to implement HSE Management Systems including HSE Cases in all Shell companies operations prompted the development of a relational data base software package (THESIS) to provide a structured way of preparing an HSE Case. The software includes features which facilitate the management of {open_quotes}Keeping the Case Alive{close_quotes}, enabling the dissemination of tasks and hazard information to the workplace. During the software development it was recognized that a significant reduction could be made in the resources which would be required to prepare an HSE Case for each and every operation by the building of {open_quotes}Generic HSE Cases{close_quotes} addressing specificmore » activities which were repeated across the Company`s operations. This was recognized to be particularly valid for the smaller Single String Venture type of operations. The activities selected for the initial Generic HSE Case development include Land Drilling Operations, Land Seismic Acquisition, and Land Transport. To establish the Generic HSE Case, the THESIS data base is populated with data for a generic operation, identifying all the hazards and activities associated with that operation including all the associated controls, with established formats for the textual sections. In effect, the Generic Case defines the standards required for that type of operation. To generate an operation specific HSE Case, the Generic Case thereafter requires to be modified/adapted so that it represents the actual situation in the operation which it defines. This process includes itemization of all the operation specific details, and may involve the inclusion/deletion of any additional/existing activities or hazards together with their associated controls.« less

78 FR 25760 - Proposed Information Collection; Urban Park and Recreation Recovery Program Grants

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-02

...--renovate or redesign existing close-to- home recreation facilities. Innovation--specific activities that... existing programs. Planning--development of a Recovery Action Program plan. The information collection... recreation system goals. Citizen involvement in the development of the Action Plan is required and may...

48 CFR 27.408 - Cosponsored research and development activities.

Code of Federal Regulations, 2011 CFR

2011-10-01

....e., Government, contractor, and/or third party paid costs), and the respective contributions are not... and development that require the contractor to make substantial contributions of funds or resources (e.g., by cost-sharing or by repayment of nonrecurring costs), and the contractor's and the Government...

75 FR 76750 - BOEMRE Information Collection Activity: 1010-0183, Information Requirements for Exploration Plans...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-09

... DEPARTMENT OF THE INTERIOR Bureau of Ocean Energy Management, Regulation and Enforcement [Docket... for Exploration Plans, Development and Production Plans, and Development Operations Coordination Documents on the OCS NTL, Renewal of a Collection; Submitted for Office of Management and Budget (OMB...

45 CFR 1304.53 - Facilities, materials, and equipment.

Code of Federal Regulations, 2012 CFR

2012-10-01

... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION FOR CHILDREN, YOUTH AND... development of each child. (2) Grantee and delegate agencies must provide appropriate space for the conduct of all program activities (see 45 CFR 1308.4 for specific access requirements for children with...

45 CFR 1304.53 - Facilities, materials, and equipment.

Code of Federal Regulations, 2014 CFR

2014-10-01

... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION FOR CHILDREN, YOUTH AND... development of each child. (2) Grantee and delegate agencies must provide appropriate space for the conduct of all program activities (see 45 CFR 1308.4 for specific access requirements for children with...

45 CFR 1304.53 - Facilities, materials, and equipment.

Code of Federal Regulations, 2013 CFR

2013-10-01

... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION FOR CHILDREN, YOUTH AND... development of each child. (2) Grantee and delegate agencies must provide appropriate space for the conduct of all program activities (see 45 CFR 1308.4 for specific access requirements for children with...

45 CFR 1304.53 - Facilities, materials, and equipment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION FOR CHILDREN, YOUTH AND... development of each child. (2) Grantee and delegate agencies must provide appropriate space for the conduct of all program activities (see 45 CFR 1308.4 for specific access requirements for children with...

24 CFR 92.210 - Troubled HOME-assisted rental housing projects.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Troubled HOME-assisted rental... of Housing and Urban Development HOME INVESTMENT PARTNERSHIPS PROGRAM Program Requirements Eligible and Prohibited Activities § 92.210 Troubled HOME-assisted rental housing projects. (a) The provisions...

Requirement of Xmsx-1 in the BMP-triggered ventralization of Xenopus embryos.

PubMed

Yamamoto, T S; Takagi, C; Ueno, N

2000-03-01

Signaling triggered by polypeptide growth factors leads to the activation of their target genes. Several homeobox genes are known to be induced in response to polypeptide growth factors in early Xenopus development. In particular, Xmsx-1, an amphibian homologue of vertebrate Msx-1, is well characterized as a target gene of bone morphogenetic protein (BMP). Here, using a dominant-negative form of Xmsx-1 (VP-Xmsx-1), which is a fusion protein made with the virus-derived VP16 activation domain, we have examined whether Xmsx-1 activity is required in the endogenous ventralizing pathway. VP-Xmsx-1 induced a secondary body axis, complete with muscle and neural tissues, when overexpressed in ventral blastomeres, suggesting that Xmsx-1 activity is necessary for both mesoderm and ectoderm to be ventralized. We have also examined the epistatic relationship between Xmsx-1 and another ventralizing homeobox protein, Xvent-1, and show that Xmsx-1 is likely to be acting upstream of Xvent-1. We propose that Xmsx-1 is required in the BMP-stimulated ventralization pathway that involves the downstream activation of Xvent-1.

Combinatorial activation and concentration-dependent repression of the Drosophila even skipped stripe 3+7 enhancer

PubMed Central

Struffi, Paolo; Corado, Maria; Kaplan, Leah; Yu, Danyang; Rushlow, Christine; Small, Stephen

2011-01-01

Despite years of study, the precise mechanisms that control position-specific gene expression during development are not understood. Here, we analyze an enhancer element from the even skipped (eve) gene, which activates and positions two stripes of expression (stripes 3 and 7) in blastoderm stage Drosophila embryos. Previous genetic studies showed that the JAK-STAT pathway is required for full activation of the enhancer, whereas the gap genes hunchback (hb) and knirps (kni) are required for placement of the boundaries of both stripes. We show that the maternal zinc-finger protein Zelda (Zld) is absolutely required for activation, and present evidence that Zld binds to multiple non-canonical sites. We also use a combination of in vitro binding experiments and bioinformatics analysis to redefine the Kni-binding motif, and mutational analysis and in vivo tests to show that Kni and Hb are dedicated repressors that function by direct DNA binding. These experiments significantly extend our understanding of how the eve enhancer integrates positive and negative transcriptional activities to generate sharp boundaries in the early embryo. PMID:21865322

A patient and public involvement (PPI) toolkit for meaningful and flexible involvement in clinical trials - a work in progress.

PubMed

Bagley, Heather J; Short, Hannah; Harman, Nicola L; Hickey, Helen R; Gamble, Carrol L; Woolfall, Kerry; Young, Bridget; Williamson, Paula R

2016-01-01

Funders of research are increasingly requiring researchers to involve patients and the public in their research. Patient and public involvement (PPI) in research can potentially help researchers make sure that the design of their research is relevant, that it is participant friendly and ethically sound. Using and sharing PPI resources can benefit those involved in undertaking PPI, but existing PPI resources are not used consistently and this can lead to duplication of effort. This paper describes how we are developing a toolkit to support clinical trials teams in a clinical trials unit. The toolkit will provide a key 'off the shelf' resource to support trial teams with limited resources, in undertaking PPI. Key activities in further developing and maintaining the toolkit are to: ● listen to the views and experience of both research teams and patient and public contributors who use the tools; ● modify the tools based on our experience of using them; ● identify the need for future tools; ● update the toolkit based on any newly identified resources that come to light; ● raise awareness of the toolkit and ● work in collaboration with others to either develop or test out PPI resources in order to reduce duplication of work in PPI. Background Patient and public involvement (PPI) in research is increasingly a funder requirement due to the potential benefits in the design of relevant, participant friendly, ethically sound research. The use and sharing of resources can benefit PPI, but available resources are not consistently used leading to duplication of effort. This paper describes a developing toolkit to support clinical trials teams to undertake effective and meaningful PPI. Methods The first phase in developing the toolkit was to describe which PPI activities should be considered in the pathway of a clinical trial and at what stage these activities should take place. This pathway was informed through review of the type and timing of PPI activities within trials coordinated by the Clinical Trials Research Centre and previously described areas of potential PPI impact in trials. In the second phase, key websites around PPI and identification of resources opportunistically, e.g. in conversation with other trialists or social media, were used to identify resources. Tools were developed where gaps existed. Results A flowchart was developed describing PPI activities that should be considered in the clinical trial pathway and the point at which these activities should happen. Three toolkit domains were identified: planning PPI; supporting PPI; recording and evaluating PPI. Four main activities and corresponding tools were identified under the planning for PPI: developing a plan; identifying patient and public contributors; allocating appropriate costs; and managing expectations. In supporting PPI, tools were developed to review participant information sheets. These tools, which require a summary of potential trial participant characteristics and circumstances help to clarify requirements and expectations of PPI review. For recording and evaluating PPI, the planned PPI interventions should be monitored in terms of impact, and a tool to monitor public contributor experience is in development. Conclusions This toolkit provides a developing 'off the shelf' resource to support trial teams with limited resources in undertaking PPI. Key activities in further developing and maintaining the toolkit are to: listen to the views and experience of both research teams and public contributors using the tools, to identify the need for future tools, to modify tools based on experience of their use; to update the toolkit based on any newly identified resources that come to light; to raise awareness of the toolkit and to work in collaboration with others to both develop and test out PPI resources in order to reduce duplication of work in PPI.

The seductive lure of creative compensation.

PubMed

Harrison, B J

2001-04-01

In today's booming economy, the experienced development professional is being asked to take a more active role in the management of the organization. Not only is the development professional required to raise money in the traditional sense, but she is also being asked to create programs that will raise money in cyberspace, develop e-commerce ventures and product lines.

GeoEnvironmental Education Through Multidisciplinary Research

NASA Astrophysics Data System (ADS)

Padilla, I. Y.; Hwang, S.

2007-12-01

The growing need to understand environmental and geological processes, their impacts, and solutions in a dynamic world requires a diverse, multidiscipline, and multicultural approach in science and engineering. In the last few years, faculty at the University of Puerto Rico, Mayagüez have engaged in education and training activities aimed at developing a critical mass of students that can address a wide range of geoenvironmental problems through multidisciplinary research. Students of diverse age, gender, culture, and academic disciplines addressing different research questions work together in a common space. Hierarchy assignments use senior students as primary mentors, but foster work at parallel levels that require sharing and developing knowledge and research resources. The activities have resulted in a significant increase in the number of diverse students in science and engineering areas related to the environment.

Standard for Models and Simulations

NASA Technical Reports Server (NTRS)

Steele, Martin J.

2016-01-01

This NASA Technical Standard establishes uniform practices in modeling and simulation to ensure essential requirements are applied to the design, development, and use of models and simulations (MS), while ensuring acceptance criteria are defined by the program project and approved by the responsible Technical Authority. It also provides an approved set of requirements, recommendations, and criteria with which MS may be developed, accepted, and used in support of NASA activities. As the MS disciplines employed and application areas involved are broad, the common aspects of MS across all NASA activities are addressed. The discipline-specific details of a given MS should be obtained from relevant recommended practices. The primary purpose is to reduce the risks associated with MS-influenced decisions by ensuring the complete communication of the credibility of MS results.

Residential development alters behavior, movement, and energetics in an apex predator, the puma

PubMed Central

Wang, Yiwei; Wilmers, Christopher C.

2017-01-01

Human development strongly influences large carnivore survival and persistence globally. Behavior changes are often the first measureable responses to human disturbances, and can have ramifications on animal populations and ecological communities. We investigated how a large carnivore responds to anthropogenic disturbances by measuring activity, movement behavior, and energetics in pumas along a housing density gradient. We used log-linear analyses to examine how habitat, time of day, and proximity to housing influenced the activity patterns of both male and female pumas in the Santa Cruz Mountains. We used spatial GPS location data in combination with Overall Dynamic Body Acceleration measurements recorded by onboard accelerometers to quantify how development density affected the average distances traveled and energy expended by pumas. Pumas responded to development differently depending on the time of day; at night, they were generally more active and moved further when they were in developed areas, but these relationships were not consistent during the day. Higher nighttime activity in developed areas increased daily caloric expenditure by 10.1% for females and 11.6% for males, resulting in increases of 3.4 and 4.0 deer prey required annually by females and males respectively. Our results support that pumas have higher energetic costs and resource requirements in human-dominated habitats due to human-induced behavioral change. Increased energetic costs for pumas are likely to have ramifications on prey species and exacerbate human-wildlife conflict, especially as exurban growth continues. Future conservation work should consider the consequences of behavioral shifts on animal energetics, individual fitness, and population viability. PMID:29020087

The California Wildlife/Fish Habitat Relationship System

Treesearch

William E. Grenfell; Hal Salwasser; William F. Laudenslayer

1982-01-01

The California Wildlife/Fish Habitat Relationships (WFHR) System is an ongoing effort to apply our knowledge of wildlife habitat requirements to identify and explain the consequences of proposed land use activities, particularly those activities that affect vegetation. The U.S. Forest Service initiated the WFHR program in California in 1976 and has developed it for all...

Increasing Children's Physical Activity: Individual, Social, and Environmental Factors Associated with Walking to and from School

ERIC Educational Resources Information Center

Trapp, Georgina S. A.; Giles-Corti, Billie; Christian, Hayley E.; Bulsara, Max; Timperio, Anna F.; McCormack, Gavin R.; Villaneuva, Karen P.

2012-01-01

Background. Efforts to increase the prevalence of children's active school transport require evidence to inform the development of comprehensive interventions. This study used a multilevel ecological framework to investigate individual, social, and environmental factors associated with walking to and from school among elementary school-aged…

Effects of gestational exposure to PFOA on PPAR protein and mRNA expression in vital organs of fetal and postnatal mice

EPA Science Inventory

Perfluorooctanoic acid (PFOA) is developmentally toxic, causing in utero and neonatal mortality, and altering development and growth in mice. PFOA activates peroxisome proliferator-activated receptor (PPAR)a and PPARa signaling is required for toxicity. This study examines the ex...

Promoting Active Learning by Practicing the "Self-Assembly" of Model Analytical Instruments

ERIC Educational Resources Information Center

Algar, W. Russ; Krull, Ulrich J.

2010-01-01

In our upper-year instrumental analytical chemistry course, we have developed "cut-and-paste" exercises where students "build" models of analytical instruments from individual schematic images of components. These exercises encourage active learning by students. Instead of trying to memorize diagrams, students are required to think deeply about…

Critical Inquiry across the Disciplines: Strategies for Student-Generated Problem Posing

ERIC Educational Resources Information Center

Nardone, Carroll Ferguson; Lee, Renee Gravois

2011-01-01

Problem posing is a higher-order, active-learning task that is important for students to develop. This article describes a series of interdisciplinary learning activities designed to help students strengthen their problem-posing skills, which requires that students become more responsible for their learning and that faculty move to a facilitator…

LOOP: Linking Outcomes to Organizational Planning, First Quarterly Report: 25 School Board Priorities.

ERIC Educational Resources Information Center

Ligon, Glynn; Wicker, Maria L. R.

The Austin Independent School District Superintendent of Schools required a process for monitoring district activities. The Office of Research and Evaluation developed LOOP (Linking Outcomes to Organizational Planning). LOOP is a management information system through which progress in a number of activities within the school system may be…

Developing a Psychology Undergraduate Research Community in a New University

ERIC Educational Resources Information Center

Roberts, Patricia; Ertubey, Candan; McMurray, Isabella; Robertson, Ian

2012-01-01

Psychology is a science-based discipline in which research is inextricably embedded in teaching and learning activities. Educators use different methods to help students in their learning of the nature of research and the practical skills required to conduct research, with students playing either a passive or more active role in the learning…

Developing Charismatic Delivery through Transformational Presentations: Modeling the Persona of Steve Jobs

ERIC Educational Resources Information Center

Ivic, Rebecca K.; Green, Robert J.

2012-01-01

How can public speaking instructors teach students how to be charismatic and confident speakers? The activity presented in this article suggests that instructors foster competent and charismatic presentational skills by having students channel the stylistic capabilities of an exceptional speaker. The activity requires students to take on the…

LDRD 2017 Annual Report: Laboratory Directed Research and Development Program Activities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anderson, Jack; Flynn, Liz

This report provides a detailed look at the scientific and technical activities for each of the LDRD projects funded by BNL in FY 2017, as required. In FY 2017, the BNL LDRD Program funded 46 projects, 13 of which were new starts, at a total cost of $10.4M.

Promoting Readiness to Practice: Which Learning Activities Promote Competence and Professional Identity for Student Social Workers during Practice Learning?

ERIC Educational Resources Information Center

Roulston, Audrey; Cleak, Helen; Vreugdenhil, Anthea

2018-01-01

Practice learning is integral to the curriculum for qualifying social work students. Accreditation standards require regular student supervision and exposure to specific learning activities. Most agencies offer high-quality placements, but organizational cutbacks may affect supervision and restrict the development of competence and professional…

Feedback on fat: p62-mTORC1-autophagy connections

PubMed Central

Moscat, Jorge; Diaz-Meco, Maria T.

2011-01-01

Metabolic homeostasis requires integration of multiple signals and cellular activities. Without this integration, conditions of obesity and diabetes often develop. Recent in vivo studies explore the molecular basis for metabolic homestasis, showing that p62 links autophagy and mTORC1 activation to regulate adipogenesis and energy control. PMID:22078874

77 FR 37903 - Agency Information Collection Activities; Submission to OMB for Review and Approval; Comment...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-25

... Activities; Submission to OMB for Review and Approval; Comment Request; Risk Management Program Requirements... information about the electronic docket, go to www.regulations.gov . Title: Risk Management Program... process develop and implement a risk management program and submit a risk management plan to EPA. The...

The Lysine Acetyltransferase GCN5 Is Required for iNKT Cell Development through EGR2 Acetylation.

PubMed

Wang, Yajun; Yun, Chawon; Gao, Beixue; Xu, Yuanming; Zhang, Yana; Wang, Yiming; Kong, Qingfei; Zhao, Fang; Wang, Chyung-Ru; Dent, Sharon Y R; Wang, Jian; Xu, Xiangping; Li, Hua-Bin; Fang, Deyu

2017-07-18

The development of CD1d-restricted invariant natural killer T (iNKT) cells, a population that is critical for both innate and adaptive immunity, is regulated by multiple transcription factors, but the molecular mechanisms underlying how the transcriptional activation of these factors are regulated during iNKT development remain largely unknown. We found that the histone acetyltransferase general control non-derepressible 5 (GCN5) is essential for iNKT cell development during the maturation stage. GCN5 deficiency blocked iNKT cell development in a cell-intrinsic manner. At the molecular level, GCN5 is a specific lysine acetyltransferase of early growth responsive gene 2 (EGR2), a transcription factor required for iNKT cell development. GCN5-mediated acetylation positively regulated EGR2 transcriptional activity, and both genetic and pharmacological GCN5 suppression specifically inhibited the transcription of EGR2 target genes in iNKT cells, including Runx1, promyelocytic leukemia zinc finger protein (PLZF), interleukin (IL)-2Rb, and T-bet. Therefore, our study revealed GCN5-mediated EGR2 acetylation as a molecular mechanism that regulates iNKT development. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

SET1A/COMPASS and shadow enhancers in the regulation of homeotic gene expression

PubMed Central

Cao, Kaixiang; Collings, Clayton K.; Marshall, Stacy A.; Morgan, Marc A.; Rendleman, Emily J.; Wang, Lu; Sze, Christie C.; Sun, Tianjiao; Bartom, Elizabeth T.; Shilatifard, Ali

2017-01-01

The homeotic (Hox) genes are highly conserved in metazoans, where they are required for various processes in development, and misregulation of their expression is associated with human cancer. In the developing embryo, Hox genes are activated sequentially in time and space according to their genomic position within Hox gene clusters. Accumulating evidence implicates both enhancer elements and noncoding RNAs in controlling this spatiotemporal expression of Hox genes, but disentangling their relative contributions is challenging. Here, we identify two cis-regulatory elements (E1 and E2) functioning as shadow enhancers to regulate the early expression of the HoxA genes. Simultaneous deletion of these shadow enhancers in embryonic stem cells leads to impaired activation of HoxA genes upon differentiation, while knockdown of a long noncoding RNA overlapping E1 has no detectable effect on their expression. Although MLL/COMPASS (complex of proteins associated with Set1) family of histone methyltransferases is known to activate transcription of Hox genes in other contexts, we found that individual inactivation of the MLL1-4/COMPASS family members has little effect on early Hox gene activation. Instead, we demonstrate that SET1A/COMPASS is required for full transcriptional activation of multiple Hox genes but functions independently of the E1 and E2 cis-regulatory elements. Our results reveal multiple regulatory layers for Hox genes to fine-tune transcriptional programs essential for development. PMID:28487406

What Is Lean Management in Health Care? Development of an Operational Definition for a Cochrane Systematic Review.

PubMed

Rotter, Thomas; Plishka, Christopher; Lawal, Adegboyega; Harrison, Liz; Sari, Nazmi; Goodridge, Donna; Flynn, Rachel; Chan, James; Fiander, Michelle; Poksinska, Bonnie; Willoughby, Keith; Kinsman, Leigh

2018-01-01

Industrial improvement approaches such as Lean management are increasingly being adopted in health care. Synthesis is necessary to ensure these approaches are evidence based and requires operationalization of concepts to ensure all relevant studies are included. This article outlines the process utilized to develop an operational definition of Lean in health care. The literature search, screening, data extraction, and data synthesis processes followed the recommendations outlined by the Cochrane Collaboration. Development of the operational definition utilized the methods prescribed by Kinsman et al. and Wieland et al. This involved extracting characteristics of Lean, synthesizing similar components to establish an operational definition, applying this definition, and updating the definition to address shortcomings. We identified two defining characteristics of Lean health-care management: (1) Lean philosophy, consisting of Lean principles and continuous improvement, and (2) Lean activities, which include Lean assessment activities and Lean improvement activities. The resulting operational definition requires that an organization or subunit of an organization had integrated Lean philosophy into the organization's mandate, guidelines, or policies and utilized at least one Lean assessment activity or Lean improvement activity. This operational definition of Lean management in health care will act as an objective screening criterion for our systematic review. To our knowledge, this is the first evidence-based operational definition of Lean management in health care.

[Physiotherapy for juvenile idiopathic arthritis].

PubMed

Spamer, M; Georgi, M; Häfner, R; Händel, H; König, M; Haas, J-P

2012-07-01

Control of disease activity and recovery of function are major issues in the treatment of children and adolescents suffering from juvenile idiopathic arthritis (JIA). Functional therapies including physiotherapy are important components in the multidisciplinary teamwork and each phase of the disease requires different strategies. While in the active phase of the disease pain alleviation is the main focus, the inactive phase requires strategies for improving motility and function. During remission the aim is to regain general fitness by sports activities. These phase adapted strategies must be individually designed and usually require a combination of different measures including physiotherapy, occupational therapy, massage as well as other physical procedures and sport therapy. There are only few controlled studies investigating the effectiveness of physical therapies in JIA and many strategies are derived from long-standing experience. New results from physiology and sport sciences have contributed to the development in recent years. This report summarizes the basics and main strategies of physical therapy in JIA.

The General Mission Analysis Tool (GMAT) System Test Plan

NASA Technical Reports Server (NTRS)

Conway, Darrel J.; Hughes, Steven P.

2007-01-01

This document serves as the System Test Approach for the GMAT Project. Preparation for system testing consists of three major stages: 1) The Test Approach sets the scope of system testing, the overall strategy to be adopted, the activities to be completed, the general resources required and the methods and processes to be used to test the release. It also details the activities, dependencies and effort required to conduct the System Test. 2) Test Planning details the activities, dependencies and effort required to conduct the System Test. 3) Test Cases documents the tests to be applied, the data to be processed, the automated testing coverage and the expected results. This document covers the first two of these items, and established the framework used for the GMAT test case development. The test cases themselves exist as separate components, and are managed outside of and concurrently with this System Test Plan.

Motives and activities for continuing professional development: An exploration of their relationships by integrating literature and interview data.

PubMed

Pool, Inge A; Poell, Rob F; Berings, Marjolein G M C; Ten Cate, Olle

2016-03-01

To effectively enhance professional development, it is important to understand the motivational factors behind nurses' engagement in particular types of learning activities. Nurses have various motives for professional development and utilise different learning activities. Not much is known about how these relate. The aim of this study was to explore the relationship between nurses' motives and activities for continuing professional development, by examining in which types of learning activities nurses engage, with which motives, and whether certain motives are associated with certain learning activities. A qualitative study using semi-structured interviews. Twenty-one nurses in academic and general Dutch hospitals participated. Interview data on nurses' learning biographies were analysed using a literature-based framework on motives and learning activities for continuing professional development. As recent classifications of nurses' motives for professional development were absent, the literature was reviewed for motives, using three databases. The interview transcripts were analysed for motives, learning activities and their relationships. Nine motives and four categories of learning activities for continuing professional development were delineated. Increasing competence was the primary motive that stimulated nurses to engage in self-directed learning during work, and in formal learning activities. To comply with requirements, they engaged in mandatory courses. To deepen knowledge, they registered for conferences. To develop their careers, they enrolled in postgraduate education. Five other motives were not mentioned as frequently. Specific motives were found to be related to engagement in particular learning activities. Nurses could use these findings to increase their awareness of why and how they develop professionally, and managers and human resource development professionals could develop approaches that would better suit nurses' needs. Copyright © 2016 Elsevier Ltd. All rights reserved.

Emergency vehicle alert system (EVAS)

NASA Technical Reports Server (NTRS)

Reed, Bill; Crump, Roger; Harper, Warren; Myneni, Krishna

1995-01-01

The Emergency Vehicle Alert System (EVAS) program is sponsored by the NASA/MSFC Technology Utilization (TU) office. The program was conceived to support the needs of hearing impaired drivers. The objective of the program is to develop a low-cost, small device which can be located in a personal vehicle and warn the driver, via a visual means, of the approach of an emergency vehicle. Many different technologies might be developed for this purpose and each has its own advantages and drawbacks. The requirements for an acoustic detection system, appear to be pretty stringent and may not allow the development of a reliable, low-cost device in the near future. The problems include variations in the sirens between various types of emergency vehicles, distortions due to wind and surrounding objects, competing background noise, sophisticated signal processing requirements, and omni-directional coverage requirements. Another approach is to use a Radio Frequency (RF) signal between the Emergency Vehicle (EV) and the Personal Vehicle (PV). This approach requires a transmitter on each EV and a receiver in each PV, however it is virtually assured that a system can be developed which works. With this approach, the real technology issue is how to make a system work as inexpensively as possible. This report gives a brief summary of the EVAS program from its inception and concentrates on describing the activities that occurred during Phase 4. References 1-3 describe activities under Phases 1-3. In the fourth phase of the program, the major effort to be expended was in development of the microcontroller system for the PV, refinement of some system elements and packaging for demonstration purposes. An EVAS system was developed and demonstrated which used standard spread spectrum modems with minor modifications.

Electrolytes with Improved Safety Characteristics for High Voltage, High Specific Energy Li-ion Cells

NASA Technical Reports Server (NTRS)

Smart, M. C.; Krause, F. C.; Hwang, C.; West, W. C.; Soler, J.; Whitcanack, L. W.; Prakash, G. K. S.; Ratnakumar, B. V.

2012-01-01

(1) NASA is actively pursuing the development of advanced electrochemical energy storage and conversion devices for future lunar and Mars missions; (2) The Exploration Technology Development Program, Energy Storage Project is sponsoring the development of advanced Li-ion batteries and PEM fuel cell and regenerative fuel cell systems for the Altair Lunar Lander, Extravehicular Activities (EVA), and rovers and as the primary energy storage system for Lunar Surface Systems; (3) At JPL, in collaboration with NASA-GRC, NASA-JSC and industry, we are actively developing advanced Li-ion batteries with improved specific energy, energy density and safety. One effort is focused upon developing Li-ion battery electrolyte with enhanced safety characteristics (i.e., low flammability); and (4) A number of commercial applications also require Li-ion batteries with enhanced safety, especially for automotive applications.

The Evaluation of Triphenyl Phosphate as a Flame Retardant Additive to Improve the Safety of Lithium-Ion Battery Electrolytes

NASA Technical Reports Server (NTRS)

Smart, M. C.; Krause, F. C.; Hwang, C.; Westa, W. C.; Soler, J.; Prakash, G. K. S.; Ratnakumar, B. V.

2011-01-01

NASA is actively pursuing the development of advanced electrochemical energy storage and conversion devices for future lunar and Mars missions. The Exploration Technology Development Program, Energy Storage Project is sponsoring the development of advanced Li-ion batteries and PEM fuel cell and regenerative fuel cell systems for the Altair Lunar Lander, Extravehicular Activities (EVA), and rovers and as the primary energy storage system for Lunar Surface Systems. At JPL, in collaboration with NASA-GRC, NASA-JSC and industry, we are actively developing advanced Li-ion batteries with improved specific energy, energy density and safety. One effort is focused upon developing Li-ion battery electrolyte with enhanced safety characteristics (i.e., low flammability). A number of commercial applications also require Li-ion batteries with enhanced safety, especially for automotive applications.

Venus Aerobot Surface Science Imaging System (VASSIS)

NASA Technical Reports Server (NTRS)

Greeley, Ronald

1999-01-01

The VASSIS task was to design and develop an imaging system and container for operation above the surface of Venus in preparation for a Discovery-class mission involving a Venus aerobot balloon. The technical goals of the effort were to: a) evaluate the possible nadir-viewed surface image quality as a function of wavelength and altitude in the Venus lower atmosphere, b) design a pressure vessel to contain the imager and supporting electronics that will meet the environmental requirements of the VASSIS mission, c) design and build a prototype imaging system including an Active-Pixel Sensor camera head and VASSIS-like optics that will meet the science requirements. The VASSIS science team developed a set of science requirements for the imaging system upon which the development work of this task was based.

Maturing Pump Technology for EVA Applications in a Collaborative Environment

NASA Technical Reports Server (NTRS)

Hodgson, Edward; Dionne, Steven; Gervais, Edward; Anchondo, Ian

2012-01-01

The transition from low earth orbit Extravehicular Activity (EVA) for construction and maintenance activities to planetary surface EVA on asteroids, moons, and, ultimately, Mars demands a new spacesuit system. NASA's development of that system has resulted in dramatically different pumping requirements from those in the current spacesuit system. Hamilton Sundstrand, Cascon, and NASA are collaborating to develop and mature a pump that will reliably meet those new requirements in space environments and within the design constraints imposed by spacesuit system integration. That collaboration, which began in the NASA purchase of a pump prototype for test evaluation, is now entering a new phase of development. A second generation pump reflecting the lessons learned in NASA's testing of the original prototype will be developed under Hamilton Sundstrand internal research funding and ultimately tested in an integrated Advanced Portable Life Support System (APLSS) in NASA laboratories at the Johnson Space Center. This partnership is providing benefit to both industry and NASA by supplying a custom component for EVA integrated testing at no cost to the government while providing test data for industry that would otherwise be difficult or impossible to duplicate in industry laboratories. This paper discusses the evolving collaborative process, component requirements and design development based on early NASA test experience, component stand alone test results, and near term plans for integrated testing at JSCs.

Modeling and control of flow during impregnation of heterogeneous porous media, with application to composite mold-filling processes

NASA Astrophysics Data System (ADS)

Bickerton, Simon

Liquid Composite Molding (LCM) encompasses a growing list of composite material manufacturing techniques. These processes have provided the promise for complex fiber reinforced plastics parts, manufactured from a single molding step. In recent years a significant research effort has been invested in development of process simulations, providing tools that have advanced current LCM technology and broadened the range of applications. The requirement for manufacture of larger, more complex parts has motivated investigation of active control of LCM processes. Due to the unlimited variety of part geometries that can be produced, finite element based process simulations will be used to some extent in design of actively controlled processes. Ongoing efforts are being made to improve material parameter specification for process simulations, increasing their value as design tools. Several phenomena occurring during mold filling have been addressed through flow visualization experimentation and analysis of manufactured composite parts. The influence of well defined air channels within a mold cavity is investigated, incorporating their effects within existing filling simulations. Three different flow configurations have been addressed, testing the application of 'equivalent permeabilities', effectively approximating air channels as representative porous media. LCM parts having doubly curved regions require preform fabrics to undergo significant, and varying deformation throughout a mold cavity. Existing methods for predicting preform deformation, and the resulting permeability distribution have been applied to a conical mold geometry. Comparisons between experiment and simulation are promising, while the geometry studied has required large deformation over much of the part, shearing the preform fabric beyond the scope of the models applied. An investigational study was performed to determine the magnitude of effect, if any, on mold filling caused by corners within LCM mold cavities. The molds applied in this study have required careful consideration of cavity thickness variations. Any effects on mold filling due to corner radii have been overshadowed by those due to preform compression. While numerical tools are available to study actively controlled mold filling in a virtual environment, some development is required for the physical equipment to implement this in practice. A versatile, multiple line fluid injection system is developed here. The equipment and control algorithms employed have provided servo control of flow rate, or injection pressure, and have been tested under very challenging conditions. The single injection line developed is expanded to a multiple line system, and shows great potential for application to actual resin systems. A case study is presented, demonstrating design and implementation of a simple actively controlled injection scheme. The experimental facility developed provides an excellent testbed for application of actively controlled mold filling concepts, an area that is providing great promise for the advancement of LCM processes.

Functional and phylogenetic analysis shows that Fgf8 is a marker of genital induction in mammals but is not required for external genital development

PubMed Central

Seifert, Ashley W.; Yamaguchi, Terry; Cohn, Martin J.

2009-01-01

Summary In mammalian embryos, male and female external genitalia develop from the genital tubercle. Outgrowth of the genital tubercle is maintained by the urethral epithelium, and it has been reported that Fgf8 mediates this activity. To test directly whether Fgf8 is required for external genital development, we conditionally removed Fgf8 from the cloacal/urethral epithelium. Surprisingly, Fgf8 is not necessary for initiation, outgrowth or normal patterning of the external genitalia. In early genital tubercles, we found no redundant Fgf expression in the urethral epithelium, which contrasts with the situation in the apical ectodermal ridge (AER) of the limb. Analysis of Fgf8 pathway activity showed that four putative targets are either absent from early genital tubercles or are not regulated by Fgf8. We therefore examined the distribution of Fgf8 protein and report that, although it is present in the AER, Fgf8 is undetectable in the genital tubercle. Thus, Fgf8 is transcribed, but the signaling pathway is not activated during normal genital development. A phylogenetic survey of amniotes revealed Fgf8 expression in genital tubercles of eutherian and metatherian mammals, but not turtles or alligators, indicating that Fgf8 expression is neither a required nor a conserved feature of amniote external genital development. The results indicate that Fgf8 expression is an early readout of the genital initiation signal rather than the signal itself. We propose that induction of external genitalia involves an epithelial-epithelial interaction at the cloacal membrane, and suggest that the cloacal ectoderm may be the source of the genital initiation signal. PMID:19592577

A Smart Wearable Sensor System for Counter-Fighting Overweight in Teenagers.

PubMed

Standoli, Carlo Emilio; Guarneri, Maria Renata; Perego, Paolo; Mazzola, Marco; Mazzola, Alessandra; Andreoni, Giuseppe

2016-08-10

PEGASO is a FP7-funded project whose goal is to develop an ICT and mobile-based platform together with an appropriate strategy to tackle the diffusion of obesity and other lifestyle-related illnesses among teenagers. Indeed, the design of an engaging strategy, leveraging a complementary set of technologies, is the approach proposed by the project to promote the adoption of healthy habits such as active lifestyle and balanced nutrition and to effectively counter-fight the emergence of overweight and obesity in the younger population. A technological key element of such a strategy sees the adoption of wearable sensors to monitor teenagers' activities, which is at the basis of developing awareness about the current lifestyle. This paper describes the experience carried out in the framework of the PEGASO project in developing and evaluating wearable monitoring systems addressed to adolescents. The paper describes the methodological approach based on the co-designing of such a wearable system and the main results that, in the first phase, involved a total of 407 adolescents across Europe in a series of focus groups conducted in three countries for the requirements definition phase. Moreover, it describes an evaluation process of signal reliability during the usage of the wearable system. The main results described here are: (a) a prototype of the standardized experimental protocol that has been developed and applied to test signal reliability in smart garments; (b) the requirements definition methodology through a co-design activity and approach to address user requirements and preferences and not only technological specifications. Such co-design approach is able to support a higher system acceptance and usability together with a sustained adoption of the solution with respect to the traditional technology push system development strategy.

A Smart Wearable Sensor System for Counter-Fighting Overweight in Teenagers

PubMed Central

Standoli, Carlo Emilio; Guarneri, Maria Renata; Perego, Paolo; Mazzola, Marco; Mazzola, Alessandra; Andreoni, Giuseppe

2016-01-01

PEGASO is a FP7-funded project whose goal is to develop an ICT and mobile-based platform together with an appropriate strategy to tackle the diffusion of obesity and other lifestyle-related illnesses among teenagers. Indeed, the design of an engaging strategy, leveraging a complementary set of technologies, is the approach proposed by the project to promote the adoption of healthy habits such as active lifestyle and balanced nutrition and to effectively counter-fight the emergence of overweight and obesity in the younger population. A technological key element of such a strategy sees the adoption of wearable sensors to monitor teenagers’ activities, which is at the basis of developing awareness about the current lifestyle. This paper describes the experience carried out in the framework of the PEGASO project in developing and evaluating wearable monitoring systems addressed to adolescents. The paper describes the methodological approach based on the co-designing of such a wearable system and the main results that, in the first phase, involved a total of 407 adolescents across Europe in a series of focus groups conducted in three countries for the requirements definition phase. Moreover, it describes an evaluation process of signal reliability during the usage of the wearable system. The main results described here are: (a) a prototype of the standardized experimental protocol that has been developed and applied to test signal reliability in smart garments; (b) the requirements definition methodology through a co-design activity and approach to address user requirements and preferences and not only technological specifications. Such co-design approach is able to support a higher system acceptance and usability together with a sustained adoption of the solution with respect to the traditional technology push system development strategy. PMID:27517929

Inductive specification and axonal orientation of spinal neurons mediated by divergent bone morphogenetic protein signaling pathways

PubMed Central

2011-01-01

Background Bone morphogenetic protein (BMP)7 evokes both inductive and axon orienting responses in dorsal interneurons (dI neurons) in the developing spinal cord. These events occur sequentially during the development of spinal neurons but in these and other cell types such inductive and acute chemotactic responses occur concurrently, highlighting the requirement for divergent intracellular signaling. Both type I and type II BMP receptor subtypes have been implicated selectively in orienting responses but it remains unclear how, in a given cell, divergence occurs. We have examined the mechanisms by which disparate BMP7 activities are generated in dorsal spinal neurons. Results We show that widely different threshold concentrations of BMP7 are required to elicit the divergent inductive and axon orienting responses. Type I BMP receptor kinase activity is required for activation of pSmad signaling and induction of dI character by BMP7, a high threshold response. In contrast, neither type I BMP receptor kinase activity nor Smad1/5/8 phosphorylation is involved in the low threshold orienting responses of dI axons to BMP7. Instead, BMP7-evoked axonal repulsion and growth cone collapse are dependent on phosphoinositide-3-kinase (PI3K) activation, plausibly through type II receptor signaling. BMP7 stimulates PI3K-dependent signaling in dI neurons. BMP6, which evokes neural induction but does not have orienting activity, activates Smad signaling but does not stimulate PI3K. Conclusions Divergent signaling through pSmad-dependent and PI3K-dependent (Smad-independent) mechanisms mediates the inductive and orienting responses of dI neurons to BMP7. A model is proposed whereby selective engagement of BMP receptor subunits underlies choice of signaling pathway. PMID:22085733