Digital servo control of random sound test excitation. [in reverberant acoustic chamber

NASA Technical Reports Server (NTRS)

Nakich, R. B. (Inventor)

1974-01-01

A digital servocontrol system for random noise excitation of a test object in a reverberant acoustic chamber employs a plurality of sensors spaced in the sound field to produce signals in separate channels which are decorrelated and averaged. The average signal is divided into a plurality of adjacent frequency bands cyclically sampled by a time division multiplex system, converted into digital form, and compared to a predetermined spectrum value stored in digital form. The results of the comparisons are used to control a time-shared up-down counter to develop gain control signals for the respective frequency bands in the spectrum of random sound energy picked up by the microphones.

Digital output compensation for precise frequency transfer over commercial fiber link

NASA Astrophysics Data System (ADS)

Ci, Cheng; Wu, Hong; Tang, Ran; Liu, Bo; Chen, Xing; Zhang, Xue-song; Zhang, Yu; Zhao, Ying-xin

2018-03-01

An ultra-highly precise and long-term stable frequency transmission system over 120 km commercial fiber link has been proposed and experimentally demonstrated. This system is based on digital output compensation technique to suppress phase fluctuations during the frequency transmission process. A mode-locked erbium-doped fiber laser driven by a hydrogen maser serves as an optical transmitter. Moreover, a dense wavelength division multiplexing system is able to separate forward and backward signals with reflection effect excluded. The ultimate fractional frequency instabilities for the long-distance frequency distributed system are up to 3.14×10-15 at 1 s and 2.96×10-19 at 10 000 s, respectively.

On-board multicarrier demodulator for mobile applications using DSP implementation

NASA Astrophysics Data System (ADS)

Yim, W. H.; Kwan, C. C. D.; Coakley, F. P.; Evans, B. G.

1990-11-01

This paper describes the design and implementation of an on-board multicarrier demodulator using commercial digital signal processors. This is for use in a mobile satellite communication system employing an up-link SCPC/FDMA scheme. Channels are separated by a flexible multistage digital filter bank followed by a channel multiplexed digital demodulator array. The cross/dot product design approach of error detector leads to a new QPSK frequency control algorithm that allows fast acquisition without special preamble pattern. Timing correction is performed digitally using an extended stack of polyphase sub-filters.

Fiber optic multiplexed optical transmission systems for space vehicle launch facilities

NASA Technical Reports Server (NTRS)

Bell, C. H.

1975-01-01

Low loss Fiber Optic Cable is being evaluated as a potential future replacement for Kennedy Space Center's 13,000 mile Wideband cable system. In order to make economical use of the wide bandwidth characteristic of glass fibers, a Frequency Division Multiplexing (FDM) scheme has been devised to stack many analog and digital data channels on a single fiber. The Multiplexed Optical Transmission System (MOTS) will offer a unique flexibility of plug-in modularity to meet changing data and bandwidth requirements in addition to the standard 'goodies' of immunity to lightning and other EMI, RFI type interferences, and of smaller size and lighter weight.

Photonic Doppler Velocimetry Multiplexing Techniques: Evaluation of Photonic Techniques

DOE Office of Scientific and Technical Information (OSTI.GOV)

Edward Daykin

This poster reports progress related to photonic technologies. Specifically, the authors developed diagnostic system architecture for a Multiplexed Photonic Doppler Velocimetry (MPDV) that incorporates frequency and time-division multiplexing into existing PDV methodology to provide increased channel count. Current MPDV design increases number of data records per digitizer channel 8x, and also operates as a laser-safe (Class 3a) system. Further, they applied heterodyne interferometry to allow for direction-of-travel determination and enable high-velocity measurements (>10 km/s) via optical downshifting. They also leveraged commercially available, inexpensive and robust components originally developed for telecom applications. Proposed MPDV architectures employ only commercially available, fiber-coupled hardware.

Frequency multiplexed superconducting quantum interference device readout of large bolometer arrays for cosmic microwave background measurements.

PubMed

Dobbs, M A; Lueker, M; Aird, K A; Bender, A N; Benson, B A; Bleem, L E; Carlstrom, J E; Chang, C L; Cho, H-M; Clarke, J; Crawford, T M; Crites, A T; Flanigan, D I; de Haan, T; George, E M; Halverson, N W; Holzapfel, W L; Hrubes, J D; Johnson, B R; Joseph, J; Keisler, R; Kennedy, J; Kermish, Z; Lanting, T M; Lee, A T; Leitch, E M; Luong-Van, D; McMahon, J J; Mehl, J; Meyer, S S; Montroy, T E; Padin, S; Plagge, T; Pryke, C; Richards, P L; Ruhl, J E; Schaffer, K K; Schwan, D; Shirokoff, E; Spieler, H G; Staniszewski, Z; Stark, A A; Vanderlinde, K; Vieira, J D; Vu, C; Westbrook, B; Williamson, R

2012-07-01

A technological milestone for experiments employing transition edge sensor bolometers operating at sub-Kelvin temperature is the deployment of detector arrays with 100s-1000s of bolometers. One key technology for such arrays is readout multiplexing: the ability to read out many sensors simultaneously on the same set of wires. This paper describes a frequency-domain multiplexed readout system which has been developed for and deployed on the APEX-SZ and South Pole Telescope millimeter wavelength receivers. In this system, the detector array is divided into modules of seven detectors, and each bolometer within the module is biased with a unique ∼MHz sinusoidal carrier such that the individual bolometer signals are well separated in frequency space. The currents from all bolometers in a module are summed together and pre-amplified with superconducting quantum interference devices operating at 4 K. Room temperature electronics demodulate the carriers to recover the bolometer signals, which are digitized separately and stored to disk. This readout system contributes little noise relative to the detectors themselves, is remarkably insensitive to unwanted microphonic excitations, and provides a technology pathway to multiplexing larger numbers of sensors.

Single-exposure super-resolved interferometric microscopy by RGB multiplexing in lensless configuration

NASA Astrophysics Data System (ADS)

Granero, Luis; Ferreira, Carlos; Zalevsky, Zeev; García, Javier; Micó, Vicente

2016-07-01

Single-Exposure Super-Resolved Interferometric Microscopy (SESRIM) reports on a way to achieve one-dimensional (1-D) superresolved imaging in digital holographic microscopy (DHM) by a single illumination shot and digital recording. SESRIM provides color-coded angular multiplexing of the accessible sample's range of spatial frequencies and it allows their recording in a single CCD (color or monochrome) snapshot by adding 3 RGB coherent reference beams at the output plane. In this manuscript, we extend the applicability of SESRIM to the field of digital in-line holographic microscopy (DIHM), that is, working without lenses. As consequence of the in-line configuration, an additional restriction concerning the object field of view (FOV) must be imposed to the technique. Experimental results are reported for both a synthetic object (USAF resolution test target) and a biological sample (swine sperm sample) validating this new kind of superresolution imaging method named as lensless SESRIM (L-SESRIM).

Time domain multiplexed spatial division multiplexing receiver.

PubMed

van Uden, Roy G H; Okonkwo, Chigo M; Chen, Haoshuo; de Waardt, Hugo; Koonen, Antonius M J

2014-05-19

A novel time domain multiplexed (TDM) spatial division multiplexing (SDM) receiver which allows for the reception of >1 dual polarization mode with a single coherent receiver, and corresponding 4-port oscilloscope, is experimentally demonstrated. Received by two coherent receivers and respective 4-port oscilloscopes, a 3 mode transmission of 28GBaud QPSK, 8, 16, and 32QAM over 41.7km of few-mode fiber demonstrates the performance of the TDM-SDM receiver with respect to back-to-back. In addition, by using carrier phase estimation employing one digital phase locked loop per output, the frequency offset between the transmitter laser and local oscillator is shown to perform similar to previous work which employs 3 coherent receivers and 4-port oscilloscopes which are dedicated to the reception of each the three modes.

A Versatile Multichannel Digital Signal Processing Module for Microcalorimeter Arrays

NASA Astrophysics Data System (ADS)

Tan, H.; Collins, J. W.; Walby, M.; Hennig, W.; Warburton, W. K.; Grudberg, P.

2012-06-01

Different techniques have been developed for reading out microcalorimeter sensor arrays: individual outputs for small arrays, and time-division or frequency-division or code-division multiplexing for large arrays. Typically, raw waveform data are first read out from the arrays using one of these techniques and then stored on computer hard drives for offline optimum filtering, leading not only to requirements for large storage space but also limitations on achievable count rate. Thus, a read-out module that is capable of processing microcalorimeter signals in real time will be highly desirable. We have developed multichannel digital signal processing electronics that are capable of on-board, real time processing of microcalorimeter sensor signals from multiplexed or individual pixel arrays. It is a 3U PXI module consisting of a standardized core processor board and a set of daughter boards. Each daughter board is designed to interface a specific type of microcalorimeter array to the core processor. The combination of the standardized core plus this set of easily designed and modified daughter boards results in a versatile data acquisition module that not only can easily expand to future detector systems, but is also low cost. In this paper, we first present the core processor/daughter board architecture, and then report the performance of an 8-channel daughter board, which digitizes individual pixel outputs at 1 MSPS with 16-bit precision. We will also introduce a time-division multiplexing type daughter board, which takes in time-division multiplexing signals through fiber-optic cables and then processes the digital signals to generate energy spectra in real time.

Optical linear algebra processors: noise and error-source modeling.

PubMed

Casasent, D; Ghosh, A

1985-06-01

The modeling of system and component noise and error sources in optical linear algebra processors (OLAP's) are considered, with attention to the frequency-multiplexed OLAP. General expressions are obtained for the output produced as a function of various component errors and noise. A digital simulator for this model is discussed.

Optical linear algebra processors - Noise and error-source modeling

NASA Technical Reports Server (NTRS)

Casasent, D.; Ghosh, A.

1985-01-01

The modeling of system and component noise and error sources in optical linear algebra processors (OLAPs) are considered, with attention to the frequency-multiplexed OLAP. General expressions are obtained for the output produced as a function of various component errors and noise. A digital simulator for this model is discussed.

Design and test of a regenerative satellite transmultiplexer

NASA Astrophysics Data System (ADS)

Hung, Kenny King-Ming

1993-05-01

In a multiple access scheme for regenerative satellite communications, the bulk frequency division multiple access (FDMA) uplink signal is demodulated on board the satellite and then remodulated for time division multiplexing (TDM) downlink transmission. Conversion from frequency to time division multiplex format requires that the uplink signal be frequency demultiplexed and each individual carrier be subsequently demodulated. For thin-route application which consists of a large number of channels with fixed data rate, multicarrier demodulation can be accomplished efficiently by a digital transmultiplexer (TMUX) using a fast Fourier transform processor followed by a bank of per-channel processors. A time domain description of the TMUX algorithm is derived which elucidates how the TMUX functions. The per-channel processor performs timing and carrier recovery for optimum and coherent data detection. Timing recovery is necessarily achieved asynchronously by a filter coefficient interpolation. Carrier recovery is performed using an all-digital phase-locked loop. The combination of both timing and carrier loops is investigated for a multi-user system. The performance of the overall system is assessed over a multi-user, additive white Gaussian noise channel for a bit energy to noise power spectral density ratio down to zero dB.

An Accurate Transmitting Power Control Method in Wireless Communication Transceivers

NASA Astrophysics Data System (ADS)

Zhang, Naikang; Wen, Zhiping; Hou, Xunping; Bi, Bo

2018-01-01

Power control circuits are widely used in transceivers aiming at stabilizing the transmitted signal power to a specified value, thereby reducing power consumption and interference to other frequency bands. In order to overcome the shortcomings of traditional modes of power control, this paper proposes an accurate signal power detection method by multiplexing the receiver and realizes transmitting power control in the digital domain. The simulation results show that this novel digital power control approach has advantages of small delay, high precision and simplified design procedure. The proposed method is applicable to transceivers working at large frequency dynamic range, and has good engineering practicability.

Digital services using quadrature amplitude modulation (QAM) over CATV analog DWDM system

NASA Astrophysics Data System (ADS)

Yeh, JengRong; Selker, Mark D.; Trail, J.; Piehler, David; Levi, Israel

2000-04-01

Dense Wavelength Division Multiplexing (DWDM) has recently gained great popularity as it provides a cost effective way to increase the transmission capacity of the existing fiber cable plant. For a long time, Dense WDM was exclusively used for baseband digital applications, predominantly in terrestrial long haul networks and in some cases in metropolitan and enterprise networks. Recently, the performance of DWDM components and frequency-stabilized lasers has substantially improved while the costs have down significantly. This makes a variety of new optical network architectures economically viable. The first commercial 8- wavelength DWDM system designed for Hybrid Fiber Coax networks was reported in 1998. This type of DWDM system utilizes Sub-Carrier Multiplexing (SCM) of Quadrature Amplitude Modulated (QAM) signals to transport IP data digital video broadcast and Video on Demand on ITU grid lightwave carriers. The ability of DWDM to provide scalable transmission capacity in the optical layer with SCM granularity is now considered by many to be the most promising technology for future transport and distribution of broadband multimedia services.

Wavelength-Division Multiplexing Of Bipolar Digital Signals

NASA Technical Reports Server (NTRS)

Gibbons, Ronnie D.; Ubele, John L., II

1994-01-01

In system, bipolar digital data transmitted by use of wavelength-division multiplexing on single optical fiber. Two different wavelengths used to transmit pulses signifying "positive" or "negative" bipolar digital data. Simultaneous absence of pulses at both wavelengths signifies digital "zero."

Seamless integration of 57.2-Gb/s signal wireline transmission and 100-GHz wireless delivery.

PubMed

Li, Xinying; Yu, Jianjun; Dong, Ze; Cao, Zizheng; Chi, Nan; Zhang, Junwen; Shao, Yufeng; Tao, Li

2012-10-22

We experimentally demonstrated the seamless integration of 57.2-Gb/s signal wireline transmission and 100-GHz wireless delivery adopting polarization-division-multiplexing quadrature-phase-shift-keying (PDM-QPSK) modulation with 400-km single-mode fiber-28 (SMF-28) transmission and 1-m wireless delivery. The X- and Y-polarization components of optical PDM-QPSK baseband signal are simultaneously up-converted to 100 GHz by optical polarization-diversity heterodyne beating, and then independently transmitted and received by two pairs of transmitter and receiver antennas, which make up a 2x2 multiple-input multiple-output (MIMO) wireless link based on microwave polarization multiplexing. At the wireless receiver, a two-stage down conversion is firstly done in analog domain based on balanced mixer and sinusoidal radio frequency (RF) signal, and then in digital domain based on digital signal processing (DSP). Polarization de-multiplexing is realized by constant modulus algorithm (CMA) based on DSP in heterodyne coherent detection. Our experimental results show that more taps are required for CMA when the X- and Y-polarization antennas have different wireless distance.

Detection of clinically relevant copy number alterations in oral cancer progression using multiplexed droplet digital PCR.

PubMed

Hughesman, Curtis B; Lu, X J David; Liu, Kelly Y P; Zhu, Yuqi; Towle, Rebecca M; Haynes, Charles; Poh, Catherine F

2017-09-19

Copy number alterations (CNAs), a common genomic event during carcinogenesis, are known to affect a large fraction of the genome. Common recurrent gains or losses of specific chromosomal regions occur at frequencies that they may be considered distinctive features of tumoral cells. Here we introduce a novel multiplexed droplet digital PCR (ddPCR) assay capable of detecting recurrent CNAs that drive tumorigenesis of oral squamous cell carcinoma. Applied to DNA extracted from oral cell lines and clinical samples of various disease stages, we found good agreement between CNAs detected by our ddPCR assay with those previously reported using comparative genomic hybridization or single nucleotide polymorphism arrays. Furthermore, we demonstrate that the ability to target specific locations of the genome permits detection of clinically relevant oncogenic events such as small, submicroscopic homozygous deletions. Additional capabilities of the multiplexed ddPCR assay include the ability to infer ploidy level, quantify the change in copy number of target loci with high-level gains, and simultaneously assess the status and viral load for high-risk human papillomavirus types 16 and 18. This novel multiplexed ddPCR assay therefore may have clinical value in differentiating between benign oral lesions from those that are at risk of progressing to oral cancer.

All-optical VPN utilizing DSP-based digital orthogonal filters access for PONs

NASA Astrophysics Data System (ADS)

Zhang, Xiaoling; Zhang, Chongfu; Chen, Chen; Jin, Wei; Qiu, Kun

2018-04-01

Utilizing digital filtering-enabled signal multiplexing and de-multiplexing, a cost-effective all-optical virtual private network (VPN) system is proposed, for the first time to our best knowledge, in digital filter multiple access passive optical networks (DFMA-PONs). Based on the DFMA technology, the proposed system can be easily designed to meet the requirements of next generation network's flexibility, elasticity, adaptability and compatibility. Through dynamic digital filter allocation and recycling, the proposed all-optical VPN system can provide dynamic establishments and cancellations of multiple VPN communications with arbitrary traffic volumes. More importantly, due to the employment of DFMA technology, the system is not limited to a fixed signal format and different signal formats such as pulse amplitude modulation (PAM), quadrature amplitude modulation (QAM) and orthogonal frequency division multiplexing (OFDM) can be used. Moreover, one transceiver is sufficient to simultaneously transmit upstream (US)/VPN data to optical line terminal (OLT) or other VPN optical network units (ONUs), thus leading to great reduction in network constructions and operation expenditures. The proposed all-optical VPN system is demonstrated with the transceiver incorporating the formats of QAM and OFDM, which can be made transparent to downstream (DS), US and VPN communications. The bit error rates (BERs) of DS, US and VPN for OFDM signals are below the forward-error-correction (FEC) limit of 3 . 8 × 10-3 when the received optical powers are about -16.8 dBm, -14.5 dBm and -15.7 dBm, respectively.

Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection

NASA Astrophysics Data System (ADS)

Dobnik, David; Štebih, Dejan; Blejec, Andrej; Morisset, Dany; Žel, Jana

2016-10-01

The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. Droplet digital PCR platforms, which include two fluorescence filters, support at least duplex reactions and with some developments and optimization higher multiplexing is possible. The present study not only shows a development of multiplex assays in droplet digital PCR, but also presents a first thorough evaluation of several parameters in such multiplex digital PCR. Two 4-plex assays were developed for quantification of 8 different DNA targets (7 genetically modified maize events and maize endogene). Per assay, two of the targets were labelled with one fluorophore and two with another. As current analysis software does not support analysis of more than duplex, a new R- and Shiny-based web application analysis tool (http://bit.ly/ddPCRmulti) was developed that automates the analysis of 4-plex results. In conclusion, the two developed multiplex assays are suitable for quantification of GMO maize events and the same approach can be used in any other field with a need for accurate and reliable quantification of multiple DNA targets.

Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection.

PubMed

Dobnik, David; Štebih, Dejan; Blejec, Andrej; Morisset, Dany; Žel, Jana

2016-10-14

The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. Droplet digital PCR platforms, which include two fluorescence filters, support at least duplex reactions and with some developments and optimization higher multiplexing is possible. The present study not only shows a development of multiplex assays in droplet digital PCR, but also presents a first thorough evaluation of several parameters in such multiplex digital PCR. Two 4-plex assays were developed for quantification of 8 different DNA targets (7 genetically modified maize events and maize endogene). Per assay, two of the targets were labelled with one fluorophore and two with another. As current analysis software does not support analysis of more than duplex, a new R- and Shiny-based web application analysis tool (http://bit.ly/ddPCRmulti) was developed that automates the analysis of 4-plex results. In conclusion, the two developed multiplex assays are suitable for quantification of GMO maize events and the same approach can be used in any other field with a need for accurate and reliable quantification of multiple DNA targets.

Superconducting Digital Multiplexers for Sensor Arrays

NASA Technical Reports Server (NTRS)

Kadin, Alan M.; Brock, Darren K.; Gupta, Deepnarayan

2004-01-01

Arrays of cryogenic microbolometers and other cryogenic detectors are being developed for infrared imaging. If the signal from each sensor is amplified, multiplexed, and digitized using superconducting electronics, then this data can be efficiently read out to ambient temperature with a minimum of noise and thermal load. HYPRES is developing an integrated system based on SQUID amplifiers, a high-resolution analog-to-digital converter (ADC) based on RSFQ (rapid single flux quantum) logic, and a clocked RSFQ multiplexer. The ADC and SQUIDs have already been demonstrated for other projects, so this paper will focus on new results of a digital multiplexer. Several test circuits have been fabricated using Nb Josephson technology and are about to be tested at T = 4.2 K, with a more complete prototype in preparation.

Fast reconstruction of off-axis digital holograms based on digital spatial multiplexing.

PubMed

Sha, Bei; Liu, Xuan; Ge, Xiao-Lu; Guo, Cheng-Shan

2014-09-22

A method for fast reconstruction of off-axis digital holograms based on digital multiplexing algorithm is proposed. Instead of the existed angular multiplexing (AM), the new method utilizes a spatial multiplexing (SM) algorithm, in which four off-axis holograms recorded in sequence are synthesized into one SM function through multiplying each hologram with a tilted plane wave and then adding them up. In comparison with the conventional methods, the SM algorithm simplifies two-dimensional (2-D) Fourier transforms (FTs) of four N*N arrays into a 1.25-D FTs of one N*N arrays. Experimental results demonstrate that, using the SM algorithm, the computational efficiency can be improved and the reconstructed wavefronts keep the same quality as those retrieved based on the existed AM method. This algorithm may be useful in design of a fast preview system of dynamic wavefront imaging in digital holography.

Estimation of vibration frequency of loudspeaker diaphragm by parallel phase-shifting digital holography

NASA Astrophysics Data System (ADS)

Kakue, T.; Endo, Y.; Shimobaba, T.; Ito, T.

2014-11-01

We report frequency estimation of loudspeaker diaphragm vibrating at high speed by parallel phase-shifting digital holography which is a technique of single-shot phase-shifting interferometry. This technique records multiple phaseshifted holograms required for phase-shifting interferometry by using space-division multiplexing. We constructed a parallel phase-shifting digital holography system consisting of a high-speed polarization-imaging camera. This camera has a micro-polarizer array which selects four linear polarization axes for 2 × 2 pixels. We set a loudspeaker as an object, and recorded vibration of diaphragm of the loudspeaker by the constructed system. By the constructed system, we demonstrated observation of vibration displacement of loudspeaker diaphragm. In this paper, we aim to estimate vibration frequency of the loudspeaker diaphragm by applying the experimental results to frequency analysis. Holograms consisting of 128 × 128 pixels were recorded at a frame rate of 262,500 frames per second by the camera. A sinusoidal wave was input to the loudspeaker via a phone connector. We observed displacement of the loudspeaker diaphragm vibrating by the system. We also succeeded in estimating vibration frequency of the loudspeaker diaphragm by applying frequency analysis to the experimental results.

Multiplexed Oversampling Digitizer in 65 nm CMOS for Column-Parallel CCD Readout

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grace, Carl; Walder, Jean-Pierre; von der Lippe, Henrik

2012-04-10

A digitizer designed to read out column-parallel charge-coupled devices (CCDs) used for high-speed X-ray imaging is presented. The digitizer is included as part of the High-Speed Image Preprocessor with Oversampling (HIPPO) integrated circuit. The digitizer module comprises a multiplexed, oversampling, 12-bit, 80 MS/s pipelined Analog-to-Digital Converter (ADC) and a bank of four fast-settling sample-and-hold amplifiers to instrument four analog channels. The ADC multiplexes and oversamples to reduce its area to allow integration that is pitch-matched to the columns of the CCD. Novel design techniques are used to enable oversampling and multiplexing with a reduced power penalty. The ADC exhibits 188more » ?V-rms noise which is less than 1 LSB at a 12-bit level. The prototype is implemented in a commercially available 65 nm CMOS process. The digitizer will lead to a proof-of-principle 2D 10 Gigapixel/s X-ray detector.« less

Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection

PubMed Central

Dobnik, David; Štebih, Dejan; Blejec, Andrej; Morisset, Dany; Žel, Jana

2016-01-01

The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. Droplet digital PCR platforms, which include two fluorescence filters, support at least duplex reactions and with some developments and optimization higher multiplexing is possible. The present study not only shows a development of multiplex assays in droplet digital PCR, but also presents a first thorough evaluation of several parameters in such multiplex digital PCR. Two 4-plex assays were developed for quantification of 8 different DNA targets (7 genetically modified maize events and maize endogene). Per assay, two of the targets were labelled with one fluorophore and two with another. As current analysis software does not support analysis of more than duplex, a new R- and Shiny-based web application analysis tool (http://bit.ly/ddPCRmulti) was developed that automates the analysis of 4-plex results. In conclusion, the two developed multiplex assays are suitable for quantification of GMO maize events and the same approach can be used in any other field with a need for accurate and reliable quantification of multiple DNA targets. PMID:27739510

Development of a wireless system for auditory neuroscience.

PubMed

Lukes, A J; Lear, A T; Snider, R K

2001-01-01

In order to study how the auditory cortex extracts communication sounds in a realistic acoustic environment, a wireless system is being developed that will transmit acoustic as well as neural signals. The miniature transmitter will be capable of transmitting two acoustic signals with 37.5 KHz bandwidths (75 KHz sample rate) and 56 neural signals with bandwidths of 9.375 KHz (18.75 KHz sample rate). These signals will be time-division multiplexed into one high bandwidth signal with a 1.2 MHz sample rate. This high bandwidth signal will then be frequency modulated onto a 2.4 GHz carrier, which resides in the industrial, scientic, and medical (ISM) band that is designed for low-power short-range wireless applications. On the receiver side, the signal will be demodulated from the 2.4 GHz carrier and then digitized by an analog-to-digital (A/D) converter. The acoustic and neural signals will be digitally demultiplexed from the multiplexed signal into their respective channels. Oversampling (20 MHz) will allow the reconstruction of the multiplexing clock by a digital signal processor (DSP) that will perform frame and bit synchronization. A frame is a subset of the signal that contains all the channels and several channels tied high and low will signal the start of a frame. This technological development will bring two benefits to auditory neuroscience. It will allow simultaneous recording of many neurons that will permit studies of population codes. It will also allow neural functions to be determined in higher auditory areas by correlating neural and acoustic signals without apriori knowledge of the necessary stimuli.

The Design of the Digital Multiplexer based on Power Carrier Communication on Sports Venues

NASA Astrophysics Data System (ADS)

Lu, Ming-jing; Liang, Li; Yu, Xiao-yan

In this paper, one kind of double CPU, the low power loss, the low cost digital multiplexer has been designed in conducted the full research to this communicated way, which is satisfied the need of the electric power correspondence transmission system, especially in sports venues. This article is elaborated the digital multiplexer's hardware and the software principle of design in detail, carries on the simulation using the monolithic integrated circuit simulator, has achieved the satisfactory effect through the debug.

Multipurpose panel, phase 1, study report. [display utilizing multiplexing and digital techniques

NASA Technical Reports Server (NTRS)

Parkin, W.

1975-01-01

The feasibility of a multipurpose panel which provides a programmable electronic display for changeable panel nomenclature, multiplexes similar indicator display signals to the signal display, and demultiplexes command signals is examined. Topics discussed include: electronic display technology, miniaturized electronic and memory devices, and data management systems which employ digital address and multiplexing.

Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging

NASA Astrophysics Data System (ADS)

Micó, Vicente; Zalevsky, Zeev

2010-07-01

Digital in-line holographic microscopy (DIHM) is a modern approach capable of achieving micron-range lateral and depth resolutions in three-dimensional imaging. DIHM in combination with numerical imaging reconstruction uses an extremely simplified setup while retaining the advantages provided by holography with enhanced capabilities derived from algorithmic digital processing. We introduce superresolved DIHM incoming from time and angular multiplexing of the sample spatial frequency information and yielding in the generation of a synthetic aperture (SA). The SA expands the cutoff frequency of the imaging system, allowing submicron resolutions in both transversal and axial directions. The proposed approach can be applied when imaging essentially transparent (low-concentration dilutions) and static (slow dynamics) samples. Validation of the method for both a synthetic object (U.S. Air Force resolution test) to quantify the resolution improvement and a biological specimen (sperm cells biosample) are reported showing the generation of high synthetic numerical aperture values working without lenses.

Optically-synchronized encoder and multiplexer scheme for interleaved photonics analog-to-digital conversion

NASA Astrophysics Data System (ADS)

Villa, Carlos; Kumavor, Patrick; Donkor, Eric

2008-04-01

Photonics Analog-to-Digital Converters (ADCs) utilize a train of optical pulses to sample an electrical input waveform applied to an electrooptic modulator or a reverse biased photodiode. In the former, the resulting train of amplitude-modulated optical pulses is detected (converter to electrical) and quantized using a conversional electronics ADC- as at present there are no practical, cost-effective optical quantizers available with performance that rival electronic quantizers. In the latter, the electrical samples are directly quantized by the electronics ADC. In both cases however, the sampling rate is limited by the speed with which the electronics ADC can quantize the electrical samples. One way to increase the sampling rate by a factor N is by using the time-interleaved technique which consists of a parallel array of N electrical ADC converters, which have the same sampling rate but different sampling phase. Each operating at a quantization rate of fs/N where fs is the aggregated sampling rate. In a system with no real-time operation, the N channels digital outputs are stored in memory, and then aggregated (multiplexed) to obtain the digital representation of the analog input waveform. Alternatively, for real-time operation systems the reduction of storing time in the multiplexing process is desired to improve the time response of the ADC. The complete elimination of memories come expenses of concurrent timing and synchronization in the aggregation of the digital signal that became critical for a good digital representation of the analog signal waveform. In this paper we propose and demonstrate a novel optically synchronized encoder and multiplexer scheme for interleaved photonics ADCs that utilize the N optical signals used to sample different phases of an analog input signal to synchronize the multiplexing of the resulting N digital output channels in a single digital output port. As a proof of concept, four 320 Megasamples/sec 12-bit of resolution digital signals were multiplexed to form an aggregated 1.28 Gigasamples/sec single digital output signal.

Ultrasensitive Detection of Multiplexed Somatic Mutations Using MALDI-TOF Mass Spectrometry.

PubMed

Mosko, Michael J; Nakorchevsky, Aleksey A; Flores, Eunice; Metzler, Heath; Ehrich, Mathias; van den Boom, Dirk J; Sherwood, James L; Nygren, Anders O H

2016-01-01

Multiplex detection of low-frequency mutations is becoming a necessary diagnostic tool for clinical laboratories interested in noninvasive prognosis and prediction. Challenges include the detection of minor alleles among abundant wild-type alleles, the heterogeneous nature of tumors, and the limited amount of available tissue. A method that can reliably detect minor variants <1% in a multiplexed reaction using a platform amenable to a variety of throughputs would meet these requirements. We developed a novel approach, UltraSEEK, for high-throughput, multiplexed, ultrasensitive mutation detection and used it for detection of mutant sequence mixtures as low as 0.1% minor allele frequency. The process consisted of multiplex PCR, followed by mutation-specific, single-base extension using chain terminators labeled with a moiety for solid phase capture. The captured and enriched products were then identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. For verification, we successfully analyzed ultralow fractions of mutations in a set of characterized cell lines, and included a direct comparison to droplet digital PCR. Finally, we verified the specificity in a set of 122 paired tumor and circulating cell-free DNA samples from melanoma patients. Our results show that the UltraSEEK chemistry is a particularly powerful approach for the detection of somatic variants, with the potential to be an invaluable resource to investigators in saving time and material without compromising analytical sensitivity and accuracy. Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

High-Speed Microscale Optical Tracking Using Digital Frequency-Domain Multiplexing.

PubMed

Maclachlan, Robert A; Riviere, Cameron N

2009-06-01

Position-sensitive detectors (PSDs), or lateral-effect photodiodes, are commonly used for high-speed, high-resolution optical position measurement. This paper describes the instrument design for multidimensional position and orientation measurement based on the simultaneous position measurement of multiple modulated sources using frequency-domain-multiplexed (FDM) PSDs. The important advantages of this optical configuration in comparison with laser/mirror combinations are that it has a large angular measurement range and allows the use of a probe that is small in comparison with the measurement volume. We review PSD characteristics and quantitative resolution limits, consider the lock-in amplifier measurement system as a communication link, discuss the application of FDM to PSDs, and make comparisons with time-domain techniques. We consider the phase-sensitive detector as a multirate DSP problem, explore parallels with Fourier spectral estimation and filter banks, discuss how to choose the modulation frequencies and sample rates that maximize channel isolation under design constraints, and describe efficient digital implementation. We also discuss hardware design considerations, sensor calibration, probe construction and calibration, and 3-D measurement by triangulation using two sensors. As an example, we characterize the resolution, speed, and accuracy of an instrument that measures the position and orientation of a 10 mm × 5 mm probe in 5 degrees of freedom (DOF) over a 30-mm cube with 4-μm peak-to-peak resolution at 1-kHz sampling.

All-optical, ultra-wideband microwave I/Q mixer and image-reject frequency down-converter.

PubMed

Gao, Yongsheng; Wen, Aijun; Chen, Wei; Li, Xiaoyan

2017-03-15

An all-optical and ultra-wideband microwave in-phase/quadrature (I/Q) mixer, based on a dual-parallel Mach-Zehnder modulator and a wavelength division multiplexer, is proposed. Due to the simultaneous frequency down-conversion and 360-deg tunable phase shifting in the optical domain, the proposed I/Q mixer has the advantages of high conversion gain and excellent quadrature phase balance (<±1.3 deg⁡) with a wide operating frequency from 10 to 40 GHz. Assisted by an analog or digital intermediate-frequency quadrature coupler, an image-reject frequency down-converter is then implemented, with an image rejection exceeding 50 dB over the working band.

Digital image compression for a 2f multiplexing optical setup

NASA Astrophysics Data System (ADS)

Vargas, J.; Amaya, D.; Rueda, E.

2016-07-01

In this work a virtual 2f multiplexing system was implemented in combination with digital image compression techniques and redundant information elimination. Depending on the image type to be multiplexed, a memory-usage saving of as much as 99% was obtained. The feasibility of the system was tested using three types of images, binary characters, QR codes, and grey level images. A multiplexing step was implemented digitally, while a demultiplexing step was implemented in a virtual 2f optical setup following real experimental parameters. To avoid cross-talk noise, each image was codified with a specially designed phase diffraction carrier that would allow the separation and relocation of the multiplexed images on the observation plane by simple light propagation. A description of the system is presented together with simulations that corroborate the method. The present work may allow future experimental implementations that will make use of all the parallel processing capabilities of optical systems.

Coherent ultra dense wavelength division multiplexing passive optical networks

NASA Astrophysics Data System (ADS)

Shahpari, Ali; Ferreira, Ricardo; Ribeiro, Vitor; Sousa, Artur; Ziaie, Somayeh; Tavares, Ana; Vujicic, Zoran; Guiomar, Fernando P.; Reis, Jacklyn D.; Pinto, Armando N.; Teixeira, António

2015-12-01

In this paper, we firstly review the progress in ultra-dense wavelength division multiplexing passive optical network (UDWDM-PON), by making use of the key attributes of this technology in the context of optical access and metro networks. Besides the inherit properties of coherent technology, we explore different modulation formats and pulse shaping. The performance is experimentally demonstrated through a 12 × 10 Gb/s bidirectional UDWDM-PON over hybrid 80 km standard single mode fiber (SSMF) and optical wireless link. High density, 6.25 GHz grid, Nyquist shaped 16-ary quadrature amplitude modulation (16QAM) and digital frequency shifting are some of the properties exploited together in the tests. Also, bidirectional transmission in fiber, relevant in the context, is analyzed in terms of nonlinear and back-reflection effects on receiver sensitivity. In addition, as a basis for the discussion on market readiness, we experimentally demonstrate real-time detection of a Nyquist-shaped quaternary phase-shift keying (QPSK) signal using simple 8-bit digital signal processing (DSP) on a field-programmable gate array (FPGA).

Developing daisy chain receivers for light-emitting diode illumination adopting the digital multiplex-512 protocol.

PubMed

Um, Keehong; Yoo, Sooyeup

2013-10-01

Protocol for digital multiplex with 512 pieces of information is increasingly adopted in the design of illumination systems. In conventional light-emitting diode systems, the receivers are connected in parallel and each of the receiving units receives all the data from the master dimmer console, but each receiving unit operates by recognizing as its own data that which corresponds to the assigned number of the receiver. Because the serial numbers of illumination devices are transmitted in binary code, synchronization is too complicated to be used properly. In order to improve the protocol of illumination control systems, we propose an algorithm of protocol reception to install and manage the system in a simpler and more convenient way. We propose the systems for controlling the light-emitting diode illumination of simplified receiver slaves adopting the digital multiplex-512 protocol where master console and multiple receiver slaves are connected in a daisy chain fashion. The digital multiplex-512 data packet is received according to the sequence order of their locations from the console, without assigning the sequence number of each channel at the receiving device. The purpose of this paper is to design a simple and small-sized controller for the control systems of lamps and lighting adopting the digital multiplex-512 network.

The DCU: the detector control unit for SPICA-SAFARI

NASA Astrophysics Data System (ADS)

Clénet, Antoine; Ravera, Laurent; Bertrand, Bernard; den Hartog, Roland H.; Jackson, Brian D.; van Leeuven, Bert-Joost; van Loon, Dennis; Parot, Yann; Pointecouteau, Etienne; Sournac, Anthony

2014-08-01

IRAP is developing the warm electronic, so called Detector Control Unit" (DCU), in charge of the readout of the SPICA-SAFARI's TES type detectors. The architecture of the electronics used to readout the 3 500 sensors of the 3 focal plane arrays is based on the frequency domain multiplexing technique (FDM). In each of the 24 detection channels the data of up to 160 pixels are multiplexed in frequency domain between 1 and 3:3 MHz. The DCU provides the AC signals to voltage-bias the detectors; it demodulates the detectors data which are readout in the cold by a SQUID; and it computes a feedback signal for the SQUID to linearize the detection chain in order to optimize its dynamic range. The feedback is computed with a specific technique, so called baseband feedback (BBFB) which ensures that the loop is stable even with long propagation and processing delays (i.e. several µs) and with fast signals (i.e. frequency carriers at 3:3 MHz). This digital signal processing is complex and has to be done at the same time for the 3 500 pixels. It thus requires an optimisation of the power consumption. We took the advantage of the relatively reduced science signal bandwidth (i.e. 20 - 40 Hz) to decouple the signal sampling frequency (10 MHz) and the data processing rate. Thanks to this method we managed to reduce the total number of operations per second and thus the power consumption of the digital processing circuit by a factor of 10. Moreover we used time multiplexing techniques to share the resources of the circuit (e.g. a single BBFB module processes 32 pixels). The current version of the firmware is under validation in a Xilinx Virtex 5 FPGA, the final version will be developed in a space qualified digital ASIC. Beyond the firmware architecture the optimization of the instrument concerns the characterization routines and the definition of the optimal parameters. Indeed the operation of the detection and readout chains requires to properly define more than 17 500 parameters (about 5 parameters per pixel). Thus it is mandatory to work out an automatic procedure to set up these optimal values. We defined a fast algorithm which characterizes the phase correction to be applied by the BBFB firmware and the pixel resonance frequencies. We also defined a technique to define the AC-carrier initial phases in such a way that the amplitude of their sum is minimized (for a better use of the DAC dynamic range).

Ultrafast FADC multiplexer

NASA Astrophysics Data System (ADS)

Mirzoyan, R.; Cortina, J.; Lorenz, E.; Martinez, M.; Ostankov, A.; Paneque, D.

2002-10-01

Ultrafast Flash amplitude-to-digital converters (FADCs) are still very expensive. Here we propose a multiplexing scheme allowing one in common trigger mode to read out multiple signal sources by using a single FADC channel. Usual coaxial cables can be used in the multiplexer as analog signal delay elements. The limited bandwidth of the coaxial cable, depending on its type and length will set an upper limit to the number of multiplexed channels. Better bandwidth and the correspondingly higher number of multiplexed channels one can obtain when using the technique of transmission of analog signals via optical fibers. Low-cost vertical cavity surface emitting laser (VCSEL) diodes can be used as converters of fast electrical signals into near infrared light. Multiplexing can be an economically priced solution when one needs ultrafast digitization of hundreds of fast signal channels.

Multiplexing 200 spatial modes with a single hologram

NASA Astrophysics Data System (ADS)

Rosales-Guzmán, Carmelo; Bhebhe, Nkosiphile; Mahonisi, Nyiku; Forbes, Andrew

2017-11-01

The on-demand tailoring of light's spatial shape is of great relevance in a wide variety of research areas. Computer-controlled devices, such as spatial light modulators (SLMs) or digital micromirror devices, offer a very accurate, flexible and fast holographic means to this end. Remarkably, digital holography affords the simultaneous generation of multiple beams (multiplexing), a tool with numerous applications in many fields. Here, we provide a self-contained tutorial on light beam multiplexing. Through the use of several examples, the readers will be guided step by step in the process of light beam shaping and multiplexing. Additionally, we provide a quantitative analysis on the multiplexing capabilities of SLMs to assess the maximum number of beams that can be multiplexed on a single SLM, showing approximately 200 modes on a single hologram.

A wideband-PCM recorder for the Space Shuttle orbiter

NASA Technical Reports Server (NTRS)

Petit, R. D.

1976-01-01

The Shuttle wideband-PCM recorder accomplishes recording on up to 14 data tracks with analog or digital data inputs. FM multiplexed analog frequencies of up to 2 MHz and digital rates of 1 Mb/s are accommodated at a tape speed of 120 in/s. Recording time in analog mode varies between 4 min for 2 MHz data to 80 min for 100 kHz data. The total digital data storage is 3.44 x 10 to the 9th bits with recording times from 1 hour for 1 Mb/s to 19 hours for 50 Kb/s data in the serial track switching mode. A versatile command decoder and control interface are used for eight primary modes of operation.

Simultaneous two-wavelength tri-window common-path digital holography

NASA Astrophysics Data System (ADS)

Liu, Lei; Shan, Mingguang; Zhong, Zhi

2018-06-01

Two-wavelength common-path off-axis digital holography is proposed with a tri-window in a single shot. It is established using a standard 4f optical image system with a 2D Ronchi grating placed outside the Fourier plane. The input plane consists of three windows: one for the object and the other two for reference. Aided by a spatial filter together with two orthogonal linear polarizers in the Fourier plane, the two-wavelength information is encoded into a multiplexed hologram with two orthogonal spatial frequencies that enable full separation of spectral information in the digital Fourier space without resolution loss. Theoretical analysis and experimental results illustrate that our approach can simultaneously perform quantitative phase imaging at two wavelengths.

Multiplexed chirp waveform synthesizer

DOEpatents

Dudley, Peter A.; Tise, Bert L.

2003-09-02

A synthesizer for generating a desired chirp signal has M parallel channels, where M is an integer greater than 1, each channel including a chirp waveform synthesizer generating at an output a portion of a digital representation of the desired chirp signal; and a multiplexer for multiplexing the M outputs to create a digital representation of the desired chirp signal. Preferably, each channel receives input information that is a function of information representing the desired chirp signal.

Real-time multiplexed digital cavity-enhanced spectroscopy

DOE PAGES

Boyson, Toby K.; Dagdigian, Paul J.; Pavey, Karl D.; ...

2015-10-01

Cavity-enhanced spectroscopy is a sensitive optical absorption technique but one where the practical applications have been limited to studying small wavelength ranges. In addition, this Letter shows that wideband operation can be achieved by combining techniques usually reserved for the communications community with that of cavity-enhanced spectroscopy, producing a multiplexed real-time cavity-enhanced spectrometer. We use multiple collinear laser sources operating asynchronously and simultaneously while being detected on a single photodetector. This is synonymous with radio frequency (RF) cellular systems in which signals are detected on a single antenna but decoded uniquely. Here, we demonstrate results with spectra of methyl salicylatemore » and show parts-per-billion per root hertz sensitivity measured in real-time.« less

A Chaos MIMO-OFDM Scheme for Mobile Communication with Physical-Layer Security

NASA Astrophysics Data System (ADS)

Okamoto, Eiji

Chaos communications enable a physical-layer security, which can enhance the transmission security in combining with upper-layer encryption techniques, or can omit the upper-layer secure protocol and enlarges the transmission efficiency. However, the chaos communication usually degrades the error rate performance compared to unencrypted digital modulations. To achieve both physical-layer security and channel coding gain, we have proposed a chaos multiple-input multiple-output (MIMO) scheme in which a rate-one chaos convolution is applied to MIMO multiplexing. However, in the conventional study only flat fading is considered. To apply this scheme to practical mobile environments, i.e., multipath fading channels, we propose a chaos MIMO-orthogonal frequency division multi-plexing (OFDM) scheme and show its effectiveness through computer simulations.

Multiplexed image storage by electromagnetically induced transparency in a solid

NASA Astrophysics Data System (ADS)

Heinze, G.; Rentzsch, N.; Halfmann, T.

2012-11-01

We report on frequency- and angle-multiplexed image storage by electromagnetically induced transparency (EIT) in a Pr3+:Y2SiO5 crystal. Frequency multiplexing by EIT relies on simultaneous storage of light pulses in atomic coherences, driven in different frequency ensembles of the inhomogeneously broadened solid medium. Angular multiplexing by EIT relies on phase matching of the driving laser beams, which permits simultaneous storage of light pulses propagating under different angles into the crystal. We apply the multiplexing techniques to increase the storage capacity of the EIT-driven optical memory, in particular to implement multiplexed storage of larger two-dimensional amounts of data (images). We demonstrate selective storage and readout of images by frequency-multiplexed EIT and angular-multiplexed EIT, as well as the potential to combine both multiplexing approaches towards further enhanced storage capacities.

Time and frequency for digital telecommunications

NASA Technical Reports Server (NTRS)

Folts, H. C.

1972-01-01

Time and frequency (T and F) are fundamental and pervasive parameters of telecommunication technology. Advancing development of digital communications using data modulation rates above 2400 baud and time-division multiplex in complex network configurations is now requiring more accurate and precise T and F reference information for efficient operation of telecommunication systems. A schematic diagram of a general communication system is shown. This diagram is very general and can depict any type of communication. The information source selects a specific message which is encoded and sent through a communication channel. Enroute, the signal is subjected to perturbations from environmental noise. The received signal is then decoded and delivered to its destination. Through the process, the message may undergo many spurious changes, resulting in a loss of information content in the delivered message as compared to the original selected message. In digital telecommunication systems, loss of information content of the signals can be attributed to noise, distortion of waveshape, and loss of synchronization.

Wired and wireless convergent extended-reach optical access network using direct-detection of all-optical OFDM super-channel signal.

PubMed

Chow, C W; Yeh, C H; Sung, J Y; Hsu, C W

2014-12-15

We propose and demonstrate the feasibility of using all-optical orthogonal frequency division multiplexing (AO-OFDM) for the convergent optical wired and wireless access networks. AO-OFDM relies on all-optically generated orthogonal subcarriers; hence, high data rate (> 100 Gb/s) can be easily achieved without hitting the speed limit of electronic digital-to-analog and analog-to-digital converters (DAC/ADC). A proof-of-concept convergent access network using AO-OFDM super-channel (SC) is demonstrated supporting 40 - 100 Gb/s wired and gigabit/s 100 GHz millimeter-wave (MMW) ROF transmissions.

Doppler compensation by shifting transmitted object frequency within limits

NASA Technical Reports Server (NTRS)

Laughlin, C. R., Jr.; Hollenbaugh, R. C.; Allen, W. K. (Inventor)

1973-01-01

A system and method are disclosed for position locating, deriving centralized air traffic control data, and communicating via voice and digital signals between a multiplicity of remote aircraft, including supersonic transports, and a central station. Such communication takes place through a synchronous satellite relay station. Side tone ranging patterns, as well as the digital and voice signals, are modulated on a carrier transmitted from the central station and received on all of the supersonic transports. Each aircraft communicates with the ground stations via a different frequency multiplexed spectrum. Supersonic transport position is derived from a computer at the central station and supplied to a local air traffic controller. Position is determined in response to variable phase information imposed on the side tones at the aircrafts. Common to all of the side tone techniques is Doppler compensation for the supersonic transport velocity.

Independent component analysis based digital signal processing in coherent optical fiber communication systems

NASA Astrophysics Data System (ADS)

Li, Xiang; Luo, Ming; Qiu, Ying; Alphones, Arokiaswami; Zhong, Wen-De; Yu, Changyuan; Yang, Qi

2018-02-01

In this paper, channel equalization techniques for coherent optical fiber transmission systems based on independent component analysis (ICA) are reviewed. The principle of ICA for blind source separation is introduced. The ICA based channel equalization after both single-mode fiber and few-mode fiber transmission for single-carrier and orthogonal frequency division multiplexing (OFDM) modulation formats are investigated, respectively. The performance comparisons with conventional channel equalization techniques are discussed.

A TDM link with channel coding and digital voice.

NASA Technical Reports Server (NTRS)

Jones, M. W.; Tu, K.; Harton, P. L.

1972-01-01

The features of a TDM (time-division multiplexed) link model are described. A PCM telemetry sequence was coded for error correction and multiplexed with a digitized voice channel. An all-digital implementation of a variable-slope delta modulation algorithm was used to digitize the voice channel. The results of extensive testing are reported. The measured coding gain and the system performance over a Gaussian channel are compared with theoretical predictions and computer simulations. Word intelligibility scores are reported as a measure of voice channel performance.

Simple Multiplexing Hand-Held Control Unit

NASA Technical Reports Server (NTRS)

Hannaford, Blake

1989-01-01

Multiplexer consists of series of resistors, each shunted by single-pole, single-throw switch. User operates switches by pressing buttons or squeezing triggers. Prototype includes three switches operated successfully in over 200 hours of system operations. Number of switches accommodated determined by signal-to-noise ratio of current source, noise induced in control unit and cable, and number of bits in output of analog-to-digital converter. Because many computer-contolled robots have extra analog-to-digital channels, such multiplexer added at little extra cost.

A Spread-Spectrum SQUID Multiplexer

NASA Astrophysics Data System (ADS)

Irwin, K. D.; Chaudhuri, S.; Cho, H.-M.; Dawson, C.; Kuenstner, S.; Li, D.; Titus, C. J.; Young, B. A.

2018-06-01

The transition-edge sensor (TES) is a mature, high-resolution x-ray spectrometer technology that provides a much higher efficiency than dispersive spectrometers such as gratings and crystal spectrometers. As larger arrays are developed, time-division multiplexing schemes operating at MHz frequencies are being replaced by microwave SQUID multiplexers using frequency-division multiplexing at GHz frequencies. However, the multiplexing factor achievable with microwave SQUIDs is limited by the high slew rate on the leading edge of x-ray pulses. In this paper, we propose a new multiplexing scheme for high-slew-rate TES x-ray calorimeters: the spread-spectrum SQUID multiplexer, which has the potential to enable higher multiplexing factors, especially in applications with lower photon-arrival rates.

Quantification of MDL-induced signal degradation in MIMO-OFDM mode-division multiplexing systems.

PubMed

Tian, Yu; Li, Juhao; Zhu, Paikun; Wu, Zhongying; Chen, Yuanxiang; He, Yongqi; Chen, Zhangyuan

2016-08-22

Mode-division multiplexing (MDM) transmission over few-mode optical fiber has emerged as a promising technology to enhance transmission capacity, in which multiple-input-multiple-output (MIMO) digital signal processing (DSP) after coherent detection is used to demultiplex the signals. Compared with conventional single-mode systems, MIMO-MDM systems suffer non-recoverable signal degradation induced by mode-dependent loss (MDL). In this paper, the MDL-induced signal degradation in orthogonal-frequency-division-multiplexing (OFDM) MDM systems is theoretically quantified in terms of mode-average error vector magnitude (EVM) through frequency domain norm analysis. A novel scalar MDL metric is proposed considering the probability distribution of the practical MDM input signals, and a closed-form expression for EVM measured after zero-force (ZF) MIMO equalization is derived. Simulation results show that the EVM estimations utilizing the novel MDL metric remain unbiased for unrepeated links. For a 6 × 100 km 20-mode MDM transmission system, the estimation accuracy is improved by more than 90% compared with that utilizing traditional condition number (CN) based MDL metric. The proposed MDL metric can be used to predict the MDL-induced SNR penalty in a theoretical manner, which will be beneficial for the design of practical MIMO-MDM systems.

Enhanced intercarrier interference mitigation based on encoded bit-sequence distribution inside optical superchannels

NASA Astrophysics Data System (ADS)

Torres, Jhon James Granada; Soto, Ana María Cárdenas; González, Neil Guerrero

2016-10-01

In the context of gridless optical multicarrier systems, we propose a method for intercarrier interference (ICI) mitigation which allows bit error correction in scenarios of nonspectral flatness between the subcarriers composing the multicarrier system and sub-Nyquist carrier spacing. We propose a hybrid ICI mitigation technique which exploits the advantages of signal equalization at both levels: the physical level for any digital and analog pulse shaping, and the bit-data level and its ability to incorporate advanced correcting codes. The concatenation of these two complementary techniques consists of a nondata-aided equalizer applied to each optical subcarrier, and a hard-decision forward error correction applied to the sequence of bits distributed along the optical subcarriers regardless of prior subchannel quality assessment as performed in orthogonal frequency-division multiplexing modulations for the implementation of the bit-loading technique. The impact of the ICI is systematically evaluated in terms of bit-error-rate as a function of the carrier frequency spacing and the roll-off factor of the digital pulse-shaping filter for a simulated 3×32-Gbaud single-polarization quadrature phase shift keying Nyquist-wavelength division multiplexing system. After the ICI mitigation, a back-to-back error-free decoding was obtained for sub-Nyquist carrier spacings of 28.5 and 30 GHz and roll-off values of 0.1 and 0.4, respectively.

Multiplexing of Hot-Electron Nanobolometers Using Microwave SQUIDs

NASA Technical Reports Server (NTRS)

Karasik, Boris S.; Day, Peter K.; Kawamura, Jonathan H.; Bumble, Bruce; LeDuc, Henry G.

2009-01-01

We have obtained the first data on the multiplexed operation of titanium hot-electron bolometers (HEB). Because of their low thermal conductance and small electron heat capacity nanobolometers are particularly interesting as sensors for far-infrared spectroscopy and mid- and near-IR calorimetry. However, the short time constant of these devices (approximately microseconds at 300-400 mK) makes time domain or audio-frequency domain multiplexing impractical. The Microwave SQUID (MSQUID) approach pursued in this work uses dc SQUIDs coupled to X-band microresonators which are, in turn, coupled to a transmission line. We used a 4-element array of Ti HEBs operated at 415 mK in a He3 dewar with an optical fiber access. The microwave signal exhibited 10-MHz wide resonances at individual MSQUD frequencies between 9 GHz and 10 GHz. The resonance depth is modulated by the current through the bolometer via a change of the SQUID flux state. The transmitted signal was amplified by a cryogenic amplifier and downconverted to baseband using an IQ mixer. A 1-dB per ??/2 responsivity was sufficient for keeping the system noise at the level of 2 pA/Hz1/2. This is more than an order of magnitude smaller than phonon noise in the HEB. The devices were able to detect single near- IR photons (1550 nm) with a time constant of 3.5 ?s. Follow-on work will scale the array to larger size and will address the microwave frequency signal generation and processing using a digital transceiver.

User's manual SIG: a general-purpose signal processing program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lager, D.; Azevedo, S.

1983-10-25

SIG is a general-purpose signal processing, analysis, and display program. Its main purpose is to perform manipulations on time- and frequency-domain signals. However, it has been designed to ultimately accommodate other representations for data such as multiplexed signals and complex matrices. Many of the basic operations one would perform on digitized data are contained in the core SIG package. Out of these core commands, more powerful signal processing algorithms may be built. Many different operations on time- and frequency-domain signals can be performed by SIG. They include operations on the samples of a signal, such as adding a scalar tomore » each sample, operations on the entire signal such as digital filtering, and operations on two or more signals such as adding two signals. Signals may be simulated, such as a pulse train or a random waveform. Graphics operations display signals and spectra.« less

A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples.

PubMed

Sedlak, Ruth Hall; Kuypers, Jane; Jerome, Keith R

2014-12-01

We demonstrate the development of a multiplex droplet digital PCR assay for human cytomegalovirus (CMV), human adenovirus species F, and an internal plasmid control that may be useful for PCR inhibition-prone clinical samples. This assay performs better on inhibition-prone stool samples than a quantitative PCR assay for CMV and is the first published clinical virology droplet digital PCR assay to incorporate an internal control. Copyright © 2014 Elsevier Inc. All rights reserved.

Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads.

PubMed

Moore, J A; Nemat-Gorgani, M; Madison, A C; Sandahl, M A; Punnamaraju, S; Eckhardt, A E; Pollack, M G; Vigneault, F; Church, G M; Fair, R B; Horowitz, M A; Griffin, P B

2017-01-01

This paper reports on the use of a digital microfluidic platform to perform multiplex automated genetic engineering (MAGE) cycles on droplets containing Escherichia coli cells. Bioactivated magnetic beads were employed for cell binding, washing, and media exchange in the preparation of electrocompetent cells in the electrowetting-on-dieletric (EWoD) platform. On-cartridge electroporation was used to deliver oligonucleotides into the cells. In addition to the optimization of a magnetic bead-based benchtop protocol for generating and transforming electrocompetent E. coli cells, we report on the implementation of this protocol in a fully automated digital microfluidic platform. Bead-based media exchange and electroporation pulse conditions were optimized on benchtop for transformation frequency to provide initial parameters for microfluidic device trials. Benchtop experiments comparing electrotransformation of free and bead-bound cells are presented. Our results suggest that dielectric shielding intrinsic to bead-bound cells significantly reduces electroporation field exposure efficiency. However, high transformation frequency can be maintained in the presence of magnetic beads through the application of more intense electroporation pulses. As a proof of concept, MAGE cycles were successfully performed on a commercial EWoD cartridge using variations of the optimal magnetic bead-based preparation procedure and pulse conditions determined by the benchtop results. Transformation frequencies up to 22% were achieved on benchtop; this frequency was matched within 1% (21%) by MAGE cycles on the microfluidic device. However, typical frequencies on the device remain lower, averaging 9% with a standard deviation of 9%. The presented results demonstrate the potential of digital microfluidics to perform complex and automated genetic engineering protocols.

Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads

PubMed Central

Moore, J. A.; Nemat-Gorgani, M.; Madison, A. C.; Punnamaraju, S.; Eckhardt, A. E.; Pollack, M. G.; Church, G. M.; Fair, R. B.; Horowitz, M. A.; Griffin, P. B.

2017-01-01

This paper reports on the use of a digital microfluidic platform to perform multiplex automated genetic engineering (MAGE) cycles on droplets containing Escherichia coli cells. Bioactivated magnetic beads were employed for cell binding, washing, and media exchange in the preparation of electrocompetent cells in the electrowetting-on-dieletric (EWoD) platform. On-cartridge electroporation was used to deliver oligonucleotides into the cells. In addition to the optimization of a magnetic bead-based benchtop protocol for generating and transforming electrocompetent E. coli cells, we report on the implementation of this protocol in a fully automated digital microfluidic platform. Bead-based media exchange and electroporation pulse conditions were optimized on benchtop for transformation frequency to provide initial parameters for microfluidic device trials. Benchtop experiments comparing electrotransformation of free and bead-bound cells are presented. Our results suggest that dielectric shielding intrinsic to bead-bound cells significantly reduces electroporation field exposure efficiency. However, high transformation frequency can be maintained in the presence of magnetic beads through the application of more intense electroporation pulses. As a proof of concept, MAGE cycles were successfully performed on a commercial EWoD cartridge using variations of the optimal magnetic bead-based preparation procedure and pulse conditions determined by the benchtop results. Transformation frequencies up to 22% were achieved on benchtop; this frequency was matched within 1% (21%) by MAGE cycles on the microfluidic device. However, typical frequencies on the device remain lower, averaging 9% with a standard deviation of 9%. The presented results demonstrate the potential of digital microfluidics to perform complex and automated genetic engineering protocols. PMID:28191268

Pico-strain multiplexed fiber optic sensor array operating down to infra-sonic frequencies.

PubMed

Littler, Ian C M; Gray, Malcolm B; Chow, Jong H; Shaddock, Daniel A; McClelland, David E

2009-06-22

An integrated sensor system is presented which displays passive long range operation to 100 km at pico-strain (pepsilon) sensitivity to low frequencies (4 Hz) in wavelength division multiplexed operation with negligible cross-talk (better than -75 dB). This has been achieved by pre-stabilizing and multiplexing all interrogation lasers for the sensor array to a single optical frequency reference. This single frequency reference allows each laser to be locked to an arbitrary wavelength and independently tuned, while maintaining suppression of laser frequency noise. With appropriate packaging, such a multiplexed strain sensing system can form the core of a low frequency accelerometer or hydrophone array.

Development of a Crosstalk Suppression Algorithm for KID Readout

NASA Astrophysics Data System (ADS)

Lee, Kyungmin; Ishitsuka, H.; Oguri, S.; Suzuki, J.; Tajima, O.; Tomita, N.; Won, Eunil; Yoshida, M.

2018-06-01

The GroundBIRD telescope aims to detect B-mode polarization of the cosmic microwave background radiation using the kinetic inductance detector array as a polarimeter. For the readout of the signal from detector array, we have developed a frequency division multiplexing readout system based on a digital down converter method. These techniques in general have the leakage problems caused by the crosstalks. The window function was applied in the field programmable gate arrays to mitigate the effect of these problems and tested it in algorithm level.

Multichannel demultiplexer/demodulator technologies for future satellite communication systems

NASA Technical Reports Server (NTRS)

Ivancic, William D.; Budinger, James M.; Staples, Edward J.; Abramovitz, Irwin; Courtois, Hector A.

1992-01-01

NASA-Lewis' Space Electronics Div. supports ongoing research in advanced satellite communication architectures, onboard processing, and technology development. Recent studies indicate that meshed VSAT (very small aperture terminal) satellite communication networks using FDMA (frequency division multiple access) uplinks and TDMA (time division multiplexed) downlinks are required to meet future communication needs. One of the critical advancements in such a satellite communication network is the multichannel demultiplexer/demodulator (MCDD). The progress is described which was made in MCDD development using either acousto-optical, optical, or digital technologies.

ICE: A Scalable, Low-Cost FPGA-Based Telescope Signal Processing and Networking System

NASA Astrophysics Data System (ADS)

Bandura, K.; Bender, A. N.; Cliche, J. F.; de Haan, T.; Dobbs, M. A.; Gilbert, A. J.; Griffin, S.; Hsyu, G.; Ittah, D.; Parra, J. Mena; Montgomery, J.; Pinsonneault-Marotte, T.; Siegel, S.; Smecher, G.; Tang, Q. Y.; Vanderlinde, K.; Whitehorn, N.

2016-03-01

We present an overview of the ‘ICE’ hardware and software framework that implements large arrays of interconnected field-programmable gate array (FPGA)-based data acquisition, signal processing and networking nodes economically. The system was conceived for application to radio, millimeter and sub-millimeter telescope readout systems that have requirements beyond typical off-the-shelf processing systems, such as careful control of interference signals produced by the digital electronics, and clocking of all elements in the system from a single precise observatory-derived oscillator. A new generation of telescopes operating at these frequency bands and designed with a vastly increased emphasis on digital signal processing to support their detector multiplexing technology or high-bandwidth correlators — data rates exceeding a terabyte per second — are becoming common. The ICE system is built around a custom FPGA motherboard that makes use of an Xilinx Kintex-7 FPGA and ARM-based co-processor. The system is specialized for specific applications through software, firmware and custom mezzanine daughter boards that interface to the FPGA through the industry-standard FPGA mezzanine card (FMC) specifications. For high density applications, the motherboards are packaged in 16-slot crates with ICE backplanes that implement a low-cost passive full-mesh network between the motherboards in a crate, allow high bandwidth interconnection between crates and enable data offload to a computer cluster. A Python-based control software library automatically detects and operates the hardware in the array. Examples of specific telescope applications of the ICE framework are presented, namely the frequency-multiplexed bolometer readout systems used for the South Pole Telescope (SPT) and Simons Array and the digitizer, F-engine, and networking engine for the Canadian Hydrogen Intensity Mapping Experiment (CHIME) and Hydrogen Intensity and Real-time Analysis eXperiment (HIRAX) radio interferometers.

Multi-channel photon counting DOT system based on digital lock-in detection technique

NASA Astrophysics Data System (ADS)

Wang, Tingting; Zhao, Huijuan; Wang, Zhichao; Hou, Shaohua; Gao, Feng

2011-02-01

Relying on deeper penetration of light in the tissue, Diffuse Optical Tomography (DOT) achieves organ-level tomography diagnosis, which can provide information on anatomical and physiological features. DOT has been widely used in imaging of breast, neonatal cerebral oxygen status and blood oxygen kinetics observed by its non-invasive, security and other advantages. Continuous wave DOT image reconstruction algorithms need the measurement of the surface distribution of the output photon flow inspired by more than one driving source, which means that source coding is necessary. The most currently used source coding in DOT is time-division multiplexing (TDM) technology, which utilizes the optical switch to switch light into optical fiber of different locations. However, in case of large amounts of the source locations or using the multi-wavelength, the measurement time with TDM and the measurement interval between different locations within the same measurement period will therefore become too long to capture the dynamic changes in real-time. In this paper, a frequency division multiplexing source coding technology is developed, which uses light sources modulated by sine waves with different frequencies incident to the imaging chamber simultaneously. Signal corresponding to an individual source is obtained from the mixed output light using digital phase-locked detection technology at the detection end. A digital lock-in detection circuit for photon counting measurement system is implemented on a FPGA development platform. A dual-channel DOT photon counting experimental system is preliminary established, including the two continuous lasers, photon counting detectors, digital lock-in detection control circuit, and codes to control the hardware and display the results. A series of experimental measurements are taken to validate the feasibility of the system. This method developed in this paper greatly accelerates the DOT system measurement, and can also obtain the multiple measurements in different source-detector locations.

Digitally enhanced homodyne interferometry.

PubMed

Sutton, Andrew J; Gerberding, Oliver; Heinzel, Gerhard; Shaddock, Daniel A

2012-09-24

We present two variations of a novel interferometry technique capable of simultaneously measuring multiple targets with high sensitivity. The technique performs a homodyne phase measurement by application of a four point phase shifting algorithm, with pseudo-random switching between points to allow multiplexed measurement based upon propagation delay alone. By multiplexing measurements and shifting complexity into signal processing, both variants realise significant complexity reductions over comparable methods. The first variant performs a typical coherent detection with a dedicated reference field and achieves a displacement noise floor 0.8 pm/√Hz above 50 Hz. The second allows for removal of the dedicated reference, resulting in further simplifications and improved low frequency performance with a 1 pm/√Hz noise floor measured down to 20 Hz. These results represent the most sensitive measurement performed using this style of interferometry whilst simultaneously reducing the electro-optic footprint.

Low-latency optical parallel adder based on a binary decision diagram with wavelength division multiplexing scheme

NASA Astrophysics Data System (ADS)

Shinya, A.; Ishihara, T.; Inoue, K.; Nozaki, K.; Kita, S.; Notomi, M.

2018-02-01

We propose an optical parallel adder based on a binary decision diagram that can calculate simply by propagating light through electrically controlled optical pass gates. The CARRY and CARRY operations are multiplexed in one circuit by a wavelength division multiplexing scheme to reduce the number of optical elements, and only a single gate constitutes the critical path for one digit calculation. The processing time reaches picoseconds per digit when we use a 100-μm-long optical path gates, which is ten times faster than a CMOS circuit.

Crosstalk in automultiscopic 3-D displays: blessing in disguise?

NASA Astrophysics Data System (ADS)

Jain, Ashish; Konrad, Janusz

2007-02-01

Most of 3-D displays suffer from interocular crosstalk, i.e., the perception of an unintended view in addition to intended one. The resulting "ghosting" at high-contrast object boundaries is objectionable and interferes with depth perception. In automultiscopic (no glasses, multiview) displays using microlenses or parallax barrier, the effect is compounded since several unintended views may be perceived at once. However, we recently discovered that crosstalk in automultiscopic displays can be also beneficial. Since spatial multiplexing of views in order to prepare a composite image for automultiscopic viewing involves sub-sampling, prior anti-alias filtering is required. To date, anti-alias filter design has ignored the presence of crosstalk in automultiscopic displays. In this paper, we propose a simple multiplexing model that takes crosstalk into account. Using this model we derive a mathematical expression for the spectrum of single view with crosstalk, and we show that it leads to reduced spectral aliasing compared to crosstalk-free case. We then propose a new criterion for the characterization of ideal anti-alias pre-filter. In the experimental part, we describe a simple method to measure optical crosstalk between views using digital camera. We use the measured crosstalk parameters to find the ideal frequency response of anti-alias filter and we design practical digital filters approximating this response. Having applied the designed filters to a number of multiview images prior to multiplexing, we conclude that, due to their increased bandwidth, the filters lead to visibly sharper 3-D images without increasing aliasing artifacts.

High-performance single cell genetic analysis using microfluidic emulsion generator arrays.

PubMed

Zeng, Yong; Novak, Richard; Shuga, Joe; Smith, Martyn T; Mathies, Richard A

2010-04-15

High-throughput genetic and phenotypic analysis at the single cell level is critical to advance our understanding of the molecular mechanisms underlying cellular function and dysfunction. Here we describe a high-performance single cell genetic analysis (SCGA) technique that combines high-throughput microfluidic emulsion generation with single cell multiplex polymerase chain reaction (PCR). Microfabricated emulsion generator array (MEGA) devices containing 4, 32, and 96 channels are developed to confer a flexible capability of generating up to 3.4 x 10(6) nanoliter-volume droplets per hour. Hybrid glass-polydimethylsiloxane diaphragm micropumps integrated into the MEGA chips afford uniform droplet formation, controlled generation frequency, and effective transportation and encapsulation of primer functionalized microbeads and cells. A multiplex single cell PCR method is developed to detect and quantify both wild type and mutant/pathogenic cells. In this method, microbeads functionalized with multiple forward primers targeting specific genes from different cell types are used for solid-phase PCR in droplets. Following PCR, the droplets are lysed and the beads are pooled and rapidly analyzed by multicolor flow cytometry. Using Escherichia coli bacterial cells as a model, we show that this technique enables digital detection of pathogenic E. coli O157 cells in a high background of normal K12 cells, with a detection limit on the order of 1/10(5). This result demonstrates that multiplex SCGA is a promising tool for high-throughput quantitative digital analysis of genetic variation in complex populations.

High-Performance Single Cell Genetic Analysis Using Microfluidic Emulsion Generator Arrays

PubMed Central

Zeng, Yong; Novak, Richard; Shuga, Joe; Smith, Martyn T.; Mathies, Richard A.

2010-01-01

High-throughput genetic and phenotypic analysis at the single cell level is critical to advance our understanding of the molecular mechanisms underlying cellular function and dysfunction. Here we describe a high-performance single cell genetic analysis (SCGA) technique that combines high-throughput microfluidic emulsion generation with single cell multiplex PCR. Microfabricated emulsion generator array (MEGA) devices containing 4, 32 and 96 channels are developed to confer a flexible capability of generating up to 3.4 × 106 nanoliter-volume droplets per hour. Hybrid glass-polydimethylsiloxane diaphragm micropumps integrated into the MEGA chips afford uniform droplet formation, controlled generation frequency, and effective transportation and encapsulation of primer functionalized microbeads and cells. A multiplex single cell PCR method is developed to detect and quantify both wild type and mutant/pathogenic cells. In this method, microbeads functionalized with multiple forward primers targeting specific genes from different cell types are used for solid-phase PCR in droplets. Following PCR, the droplets are lysed, the beads are pooled and rapidly analyzed by multi-color flow cytometry. Using E. coli bacterial cells as a model, we show that this technique enables digital detection of pathogenic E. coli O157 cells in a high background of normal K12 cells, with a detection limit on the order of 1:105. This result demonstrates that multiplex SCGA is a promising tool for high-throughput quantitative digital analysis of genetic variation in complex populations. PMID:20192178

Electro-optic architecture (EOA) for sensors and actuators in aircraft propulsion systems

NASA Technical Reports Server (NTRS)

Glomb, W. L., Jr.

1989-01-01

Results of a study to design an optimal architecture for electro-optical sensing and control in advanced aircraft and space systems are described. The propulsion full authority digital Electronic Engine Control (EEC) was the focus for the study. The recommended architecture is an on-engine EEC which contains electro-optic interface circuits for fiber-optic sensors on the engine. Size and weight are reduced by multiplexing arrays of functionally similar sensors on a pair of optical fibers to common electro-optical interfaces. The architecture contains common, multiplex interfaces to seven sensor groups: (1) self luminous sensors; (2) high temperatures; (3) low temperatures; (4) speeds and flows; (5) vibration; (6) pressures; and (7) mechanical positions. Nine distinct fiber-optic sensor types were found to provide these sensing functions: (1) continuous wave (CW) intensity modulators; (2) time division multiplexing (TDM) digital optic codeplates; (3) time division multiplexing (TDM) analog self-referenced sensors; (4) wavelength division multiplexing (WDM) digital optic code plates; (5) wavelength division multiplexing (WDM) analog self-referenced intensity modulators; (6) analog optical spectral shifters; (7) self-luminous bodies; (8) coherent optical interferometers; and (9) remote electrical sensors. The report includes the results of a trade study including engine sensor requirements, environment, the basic sensor types, and relevant evaluation criteria. These figures of merit for the candidate interface types were calculated from the data supplied by leading manufacturers of fiber-optic sensors.

Broadband quantitative phase microscopy with extended field of view using off-axis interferometric multiplexing.

PubMed

Girshovitz, Pinhas; Frenklach, Irena; Shaked, Natan T

2015-11-01

We propose a new portable imaging configuration that can double the field of view (FOV) of existing off-axis interferometric imaging setups, including broadband off-axis interferometers. This configuration is attached at the output port of the off-axis interferometer and optically creates a multiplexed interferogram on the digital camera, which is composed of two off-axis interferograms with straight fringes at orthogonal directions. Each of these interferograms contains a different FOV of the imaged sample. Due to the separation of these two FOVs in the spatial-frequency domain, they can be fully reconstructed separately, while obtaining two complex wavefronts from the sample at once. Since the optically multiplexed off-axis interferogram is recorded by the camera in a single exposure, fast dynamics can be recorded with a doubled imaging area. We used this technique for quantitative phase microscopy of biological samples with extended FOV. We demonstrate attaching the proposed module to a diffractive phase microscopy interferometer, illuminated by a broadband light source. The biological samples used for the experimental demonstrations include microscopic diatom shells, cancer cells, and flowing blood cells.

Ultra-high-speed optical transmission using digital-preprocessed analog-multiplexed DAC

NASA Astrophysics Data System (ADS)

Yamazaki, Hiroshi; Nagatani, Munehiko; Hamaoka, Fukutaro; Horikoshi, Kengo; Nakamura, Masanori; Matsushita, Asuka; Kanazawa, Shigeru; Hashimoto, Toshikazu; Nosaka, Hideyuki; Miyamoto, Yutaka

2018-02-01

In advanced fiber transmission systems with digital signal processors (DSPs), analog bandwidths of digital-to-analog converters (DACs), which interface the DSPs and optics, are the major factors limiting the data rates. We have developed a technology to extend the DACs' bandwidth using a digital preprocessor, two sub-DACs, and an analog multiplexer. This technology enables us to generate baseband signals with bandwidths of up to around 60 GHz, which is almost twice that of signals generated by typical CMOS DACs. In this paper, we describe the principle of the bandwidth extension and review high-speed transmission experiments enabled by this technology.

An Improved Model Predictive Current Controller of Switched Reluctance Machines Using Time-Multiplexed Current Sensor

PubMed Central

Li, Bingchu; Ling, Xiao; Huang, Yixiang; Gong, Liang; Liu, Chengliang

2017-01-01

This paper presents a fixed-switching-frequency model predictive current controller using multiplexed current sensor for switched reluctance machine (SRM) drives. The converter was modified to distinguish currents from simultaneously excited phases during the sampling period. The only current sensor installed in the converter was time division multiplexing for phase current sampling. During the commutation stage, the control steps of adjacent phases were shifted so that sampling time was staggered. The maximum and minimum duty ratio of pulse width modulation (PWM) was limited to keep enough sampling time for analog-to-digital (A/D) conversion. Current sensor multiplexing was realized without complex adjustment of either driver circuit nor control algorithms, while it helps to reduce the cost and errors introduced in current sampling due to inconsistency between sensors. The proposed controller is validated by both simulation and experimental results with a 1.5 kW three-phase 12/8 SRM. Satisfied current sampling is received with little difference compared with independent phase current sensors for each phase. The proposed controller tracks the reference current profile as accurately as the model predictive current controller with independent phase current sensors, while having minor tracking errors compared with a hysteresis current controller. PMID:28513554

Variant Profiling of Candidate Genes in Pancreatic Ductal Adenocarcinoma.

PubMed

Huang, Jiaqi; Löhr, Johannes-Matthias; Nilsson, Magnus; Segersvärd, Ralf; Matsson, Hans; Verbeke, Caroline; Heuchel, Rainer; Kere, Juha; Iafrate, A John; Zheng, Zongli; Ye, Weimin

2015-11-01

Pancreatic ductal adenocarcinoma (PDAC) has a poor prognosis. Variant profiling is crucial for developing personalized treatment and elucidating the etiology of this disease. Patients with PDAC undergoing surgery from 2007 to 2012 (n = 73) were followed from diagnosis until death or the end of the study. We applied an anchored multiplex PCR (AMP)-based next-generation sequencing (NGS) method to a panel of 65 selected genes and assessed analytical performance by sequencing a quantitative multiplex DNA reference standard. In clinical PDAC samples, detection of low-level KRAS (Kirsten rat sarcoma viral oncogene homolog) mutations was validated by allele-specific PCR and digital PCR. We compared overall survival of patients according to KRAS mutation status by log-rank test and applied logistic regression to evaluate the association between smoking and tumor variant types. The AMP-based NGS method could detect variants with allele frequencies as low as 1% given sufficient sequencing depth (>1500×). Low-frequency KRAS G12 mutations (allele frequency 1%-5%) were all confirmed by allele-specific PCR and digital PCR. The most prevalent genetic alterations were in KRAS (78% of patients), TP53 (tumor protein p53) (25%), and SMAD4 (SMAD family member 4) (8%). Overall survival in T3-stage PDAC patients differed among KRAS mutation subtypes (P = 0.019). Transversion variants were more common in ever-smokers than in never-smokers (odds ratio 5.7; 95% CI 1.2-27.8). The AMP-based NGS method is applicable for profiling tumor variants. Using this approach, we demonstrated that in PDAC patients, KRAS mutant subtype G12V is associated with poorer survival, and that transversion variants are more common among smokers. © 2015 American Association for Clinical Chemistry.

Digital transceiver implementation for wavelet packet modulation

NASA Astrophysics Data System (ADS)

Lindsey, Alan R.; Dill, Jeffrey C.

1998-03-01

Current transceiver designs for wavelet-based communication systems are typically reliant on analog waveform synthesis, however, digital processing is an important part of the eventual success of these techniques. In this paper, a transceiver implementation is introduced for the recently introduced wavelet packet modulation scheme which moves the analog processing as far as possible toward the antenna. The transceiver is based on the discrete wavelet packet transform which incorporates level and node parameters for generalized computation of wavelet packets. In this transform no particular structure is imposed on the filter bank save dyadic branching, and a maximum level which is specified a priori and dependent mainly on speed and/or cost considerations. The transmitter/receiver structure takes a binary sequence as input and, based on the desired time- frequency partitioning, processes the signal through demultiplexing, synthesis, analysis, multiplexing and data determination completely in the digital domain - with exception of conversion in and out of the analog domain for transmission.

Active Microelectronic Neurosensor Arrays for Implantable Brain Communication Interfaces

PubMed Central

Song, Y.-K.; Borton, D. A.; Park, S.; Patterson, W. R.; Bull, C. W.; Laiwalla, F.; Mislow, J.; Simeral, J. D.; Donoghue, J. P.; Nurmikko, A. V.

2010-01-01

We have built a wireless implantable microelectronic device for transmitting cortical signals transcutaneously. The device is aimed at interfacing a microelectrode array cortical to an external computer for neural control applications. Our implantable microsystem enables presently 16-channel broadband neural recording in a non-human primate brain by converting these signals to a digital stream of infrared light pulses for transmission through the skin. The implantable unit employs a flexible polymer substrate onto which we have integrated ultra-low power amplification with analog multiplexing, an analog-to-digital converter, a low power digital controller chip, and infrared telemetry. The scalable 16-channel microsystem can employ any of several modalities of power supply, including via radio frequency by induction, or infrared light via a photovoltaic converter. As of today, the implant has been tested as a sub-chronic unit in non-human primates (~ 1 month), yielding robust spike and broadband neural data on all available channels. PMID:19502132

Optical Signal Processing: Poisson Image Restoration and Shearing Interferometry

NASA Technical Reports Server (NTRS)

Hong, Yie-Ming

1973-01-01

Optical signal processing can be performed in either digital or analog systems. Digital computers and coherent optical systems are discussed as they are used in optical signal processing. Topics include: image restoration; phase-object visualization; image contrast reversal; optical computation; image multiplexing; and fabrication of spatial filters. Digital optical data processing deals with restoration of images degraded by signal-dependent noise. When the input data of an image restoration system are the numbers of photoelectrons received from various areas of a photosensitive surface, the data are Poisson distributed with mean values proportional to the illuminance of the incoherently radiating object and background light. Optical signal processing using coherent optical systems is also discussed. Following a brief review of the pertinent details of Ronchi's diffraction grating interferometer, moire effect, carrier-frequency photography, and achromatic holography, two new shearing interferometers based on them are presented. Both interferometers can produce variable shear.

Frequency transfer via a two-way optical phase comparison on a multiplexed fiber network.

PubMed

Calosso, C E; Bertacco, E; Calonico, D; Clivati, C; Costanzo, G A; Frittelli, M; Levi, F; Mura, A; Godone, A

2014-03-01

We performed a two-way remote optical phase comparison on optical fiber. Two optical frequency signals were launched in opposite directions in an optical fiber and their phases were simultaneously measured at the other end. In this technique, the fiber noise is passively canceled, and we compared two optical frequencies at the ultimate 10(-21) stability level. The experiment was performed on a 47 km fiber that is part of the metropolitan network for Internet traffic. The technique relies on the synchronous measurement of the optical phases at the two ends of the link, which is here performed by digital electronics. This scheme offers some advantages with respect to active noise cancellation schemes, as the light travels only once in the fiber.

Novel MDM-PON scheme utilizing self-homodyne detection for high-speed/capacity access networks.

PubMed

Chen, Yuanxiang; Li, Juhao; Zhu, Paikun; Wu, Zhongying; Zhou, Peng; Tian, Yu; Ren, Fang; Yu, Jinyi; Ge, Dawei; Chen, Jingbiao; He, Yongqi; Chen, Zhangyuan

2015-12-14

In this paper, we propose a cost-effective, energy-saving mode-division-multiplexing passive optical network (MDM-PON) scheme utilizing self-homodyne detection for high-speed/capacity access network based on low modal-crosstalk few-mode fiber (FMF) and all-fiber mode multiplexer/demultiplexer (MUX/DEMUX). In the proposed scheme, one of the spatial modes is used to transmit a portion of signal carrier (namely pilot-tone) as the local oscillator (LO), while the others are used for signal-bearing channels. At the receiver, the pilot-tone and the signal can be separated without strong crosstalk and sent to the receiver for coherent detection. The spectral efficiency (SE) is significantly enhanced when multiple spatial channels are used. Meanwhile, the self-homodyne detection scheme can effectively suppress laser phase noise, which relaxes the requirement for the lasers line-width at the optical line terminal or optical network units (OLT/ONUs). The digital signal processing (DSP) at the receiver is also simplified since it removes the need for frequency offset compensation and complex phase correction, which reduces the computational complexity and energy consumption. Polarization division multiplexing (PDM) that offers doubled SE is also supported by the scheme. The proposed scheme is scalable to multi-wavelength application when wavelength MUX/DEMUX is utilized. Utilizing the proposed scheme, we demonstrate a proof of concept 4 × 40-Gb/s orthogonal frequency division multiplexing (OFDM) transmission over 55-km FMF using low modal-crosstalk two-mode FMF and MUX/DEMUX with error free operation. Compared with back to back case, less than 1-dB Q-factor penalty is observed after 55-km FMF of the four channels. Signal power and pilot-tone power are also optimized to achieve the optimal transmission performance.

Front-end multiplexing—applied to SQUID multiplexing: Athena X-IFU and QUBIC experiments

NASA Astrophysics Data System (ADS)

Prele, D.

2015-08-01

As we have seen for digital camera market and a sensor resolution increasing to "megapixels", all the scientific and high-tech imagers (whatever the wave length - from radio to X-ray range) tends also to always increases the pixels number. So the constraints on front-end signals transmission increase too. An almost unavoidable solution to simplify integration of large arrays of pixels is front-end multiplexing. Moreover, "simple" and "efficient" techniques allow integration of read-out multiplexers in the focal plane itself. For instance, CCD (Charge Coupled Device) technology has boost number of pixels in digital camera. Indeed, this is exactly a planar technology which integrates both the sensors and a front-end multiplexed readout. In this context, front-end multiplexing techniques will be discussed for a better understanding of their advantages and their limits. Finally, the cases of astronomical instruments in the millimeter and in the X-ray ranges using SQUID (Superconducting QUantum Interference Device) will be described.

Digital barcodes of suspension array using laser induced breakdown spectroscopy

PubMed Central

He, Qinghua; Liu, Yixi; He, Yonghong; Zhu, Liang; Zhang, Yilong; Shen, Zhiyuan

2016-01-01

We show a coding method of suspension array based on the laser induced breakdown spectroscopy (LIBS), which promotes the barcodes from analog to digital. As the foundation of digital optical barcodes, nanocrystals encoded microspheres are prepared with self-assembly encapsulation method. We confirm that digital multiplexing of LIBS-based coding method becomes feasible since the microsphere can be coded with direct read-out data of wavelengths, and the method can avoid fluorescence signal crosstalk between barcodes and analyte tags, which lead to overall advantages in accuracy and stability to current fluorescent multicolor coding method. This demonstration increases the capability of multiplexed detection and accurate filtrating, expanding more extensive applications of suspension array in life science. PMID:27808270

Generation of optical OFDM signals using 21.4 GS/s real time digital signal processing.

PubMed

Benlachtar, Yannis; Watts, Philip M; Bouziane, Rachid; Milder, Peter; Rangaraj, Deepak; Cartolano, Anthony; Koutsoyannis, Robert; Hoe, James C; Püschel, Markus; Glick, Madeleine; Killey, Robert I

2009-09-28

We demonstrate a field programmable gate array (FPGA) based optical orthogonal frequency division multiplexing (OFDM) transmitter implementing real time digital signal processing at a sample rate of 21.4 GS/s. The QPSK-OFDM signal is generated using an 8 bit, 128 point inverse fast Fourier transform (IFFT) core, performing one transform per clock cycle at a clock speed of 167.2 MHz and can be deployed with either a direct-detection or a coherent receiver. The hardware design and the main digital signal processing functions are described, and we show that the main performance limitation is due to the low (4-bit) resolution of the digital-to-analog converter (DAC) and the 8-bit resolution of the IFFT core used. We analyze the back-to-back performance of the transmitter generating an 8.36 Gb/s optical single sideband (SSB) OFDM signal using digital up-conversion, suitable for direct-detection. Additionally, we use the device to transmit 8.36 Gb/s SSB OFDM signals over 200 km of uncompensated standard single mode fiber achieving an overall BER<10(-3).

Interferometric Reflectance Imaging Sensor (IRIS)—A Platform Technology for Multiplexed Diagnostics and Digital Detection

PubMed Central

Avci, Oguzhan; Lortlar Ünlü, Nese; Yalçın Özkumur, Ayça; Ünlü, M. Selim

2015-01-01

Over the last decade, the growing need in disease diagnostics has stimulated rapid development of new technologies with unprecedented capabilities. Recent emerging infectious diseases and epidemics have revealed the shortcomings of existing diagnostics tools, and the necessity for further improvements. Optical biosensors can lay the foundations for future generation diagnostics by providing means to detect biomarkers in a highly sensitive, specific, quantitative and multiplexed fashion. Here, we review an optical sensing technology, Interferometric Reflectance Imaging Sensor (IRIS), and the relevant features of this multifunctional platform for quantitative, label-free and dynamic detection. We discuss two distinct modalities for IRIS: (i) low-magnification (ensemble biomolecular mass measurements) and (ii) high-magnification (digital detection of individual nanoparticles) along with their applications, including label-free detection of multiplexed protein chips, measurement of single nucleotide polymorphism, quantification of transcription factor DNA binding, and high sensitivity digital sensing and characterization of nanoparticles and viruses. PMID:26205273

Multicarrier orthogonal spread-spectrum (MOSS) data communications

DOEpatents

Smith, Stephen F [London, TN; Dress, William B [Camas, WA

2008-01-01

Systems and methods are described for multicarrier orthogonal spread-spectrum (MOSS) data communication. A method includes individually spread-spectrum modulating at least two of a set of orthogonal frequency division multiplexed carriers, wherein the resulting individually spread-spectrum modulated at least two of a set of orthogonal frequency division multiplexed carriers are substantially mutually orthogonal with respect to both frequency division multiplexing and spread-spectrum modulation.

Unified tensor model for space-frequency spreading-multiplexing (SFSM) MIMO communication systems

NASA Astrophysics Data System (ADS)

de Almeida, André LF; Favier, Gérard

2013-12-01

This paper presents a unified tensor model for space-frequency spreading-multiplexing (SFSM) multiple-input multiple-output (MIMO) wireless communication systems that combine space- and frequency-domain spreadings, followed by a space-frequency multiplexing. Spreading across space (transmit antennas) and frequency (subcarriers) adds resilience against deep channel fades and provides space and frequency diversities, while orthogonal space-frequency multiplexing enables multi-stream transmission. We adopt a tensor-based formulation for the proposed SFSM MIMO system that incorporates space, frequency, time, and code dimensions by means of the parallel factor model. The developed SFSM tensor model unifies the tensorial formulation of some existing multiple-access/multicarrier MIMO signaling schemes as special cases, while revealing interesting tradeoffs due to combined space, frequency, and time diversities which are of practical relevance for joint symbol-channel-code estimation. The performance of the proposed SFSM MIMO system using either a zero forcing receiver or a semi-blind tensor-based receiver is illustrated by means of computer simulation results under realistic channel and system parameters.

Code and codeless ionospheric measurements with NASA's Rogue GPS Receiver

NASA Technical Reports Server (NTRS)

Srinivasan, Jeff M.; Meehan, Tom K.; Young, Lawrence E.

1989-01-01

The NASA/JPL Rogue Receiver is an 8-satellite, non-multiplexed, highly digital global positioning system (GPS) receiver that can obtain dual frequency data either with or without knowledge of the P-code. In addition to its applications for high accuracy geodesy and orbit determination, the Rogue uses GPS satellite signals to measure the total electron content (TEC) of the ionosphere along the lines of sight from the receiver to the satellites. These measurements are used by JPL's Deep Space Network (DSN) for calibrating radiometric data. This paper will discuss Rogue TEC measurements, emphasizing the advantages of a receiver that can use the P-code, when available, but can also obtain reliable dual frequency data when the code is encrypted.

All-digital multicarrier demodulators for on-board processing satellites in mobile communication systems

NASA Astrophysics Data System (ADS)

Yim, Wan Hung

Economical operation of future satellite systems for mobile communications can only be fulfilled by using dedicated on-board processing satellites, which would allow both cheap earth terminals and lower space segment costs. With on-board modems and codecs, the up-link and down-link can be optimized separately. An attractive scheme is to use frequency-division multiple access/single chanel per carrier (FDMA/SCPC) on the up-link and time division multiplexing (TDM) on the down-link. This scheme allows mobile terminals to transmit a narrow band, low power signal, resulting in smaller dishes and high power amplifiers (HPA's) with lower output power. On the up-link, there are hundreds to thousands of FDM channels to be demodulated on-board. The most promising approach is the use of all-digital multicarrier demodulators (MCD's), where analog and digital hardware are efficiently shared among channels, and digital signal processing (DSP) is used at an early stage to take advantage of very large scale integration (VLSI) implementation. A MCD consists of a channellizer for separation of frequency division multiplexing (FDM) channels, followed by individual modulators for each channel. Major research areas in MCD's are in multirate DSP, and the optimal estimation for synchronization, which form the basis of the thesis. Complex signal theories are central to the development of structured approaches for the sampling and processing of bandpass signals, which are the foundations in both channellizer and demodulator design. In multirate DSP, polyphase theories replace many ad-hoc, tedious and error-prone design procedures. For example, a polyphase-matrix deep space network frequency and timing system (DFT) channellizer includes all efficient filter bank techniques as special cases. Also, a polyphase-lattice filter is derived, not only for sampling rate conversion, but also capable of sampling phase variation, which is required for symbol timing adjustment in all-digital demodulators. In modulation schemes, a systematic survey is reported, based on two expressions that includes all formats in linear and constant envelope modulation. In synchronization techniques, classifications according to the criterion of statistical optimization, the data dependecy, and the method of parameter extraction, reflect the inherent complexity and performance of numerous existing algorithms. The designs of two new algorithms are presented: a differential decision frequency error detector that is simple and fast; a dual-comb-filter frequency/timing error detector that is targeted at VLSI implementation. The real-time implementation of a complete 4 x 16 kb/s MCD for the T-SAT project is described in detail, which proved many of the structured design concepts developed in this thesis. The requirements of software tools for various levels of simulation in multirate DSP and communications are analyzed. This led to the implementation of a data-flow oriented simulation system, which was used in all research work in the thesis.

Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events.

PubMed

Dobnik, David; Spilsberg, Bjørn; Bogožalec Košir, Alexandra; Štebih, Dejan; Morisset, Dany; Holst-Jensen, Arne; Žel, Jana

2018-01-01

The standard-curve based simplex quantitative polymerase chain reaction (qPCR) has been the gold standard for DNA target quantification for more than a decade. The large and growing number of individual analyses needed to test for genetically modified organisms (GMOs) is reducing the cost-effectiveness of qPCR. Droplet digital PCR (ddPCR) enables absolute quantification without standard curves, avoids the amplification efficiency bias observed with qPCR, allows more accurate estimations at low target copy numbers and, in combination with multiplexing, significantly improves cost efficiency. Here we describe two protocols for multiplex quantification of GM maize events: (1) nondiscriminating, with multiplex quantification of targets as a group (12 GM maize lines) and (2) discriminating, with multiplex quantification of individual targets (events). The first enables the quantification of twelve European Union authorized GM maize events as a group with only two assays, but does not permit determination of the individual events present. The second protocol enables the quantification of four individual targets (three GM events and one endogene) in a single reaction. Both protocols can be modified for quantification of any other DNA target.

Methods for Multiplex Template Sampling in Digital PCR Assays

PubMed Central

Petriv, Oleh I.; Heyries, Kevin A.; VanInsberghe, Michael; Walker, David; Hansen, Carl L.

2014-01-01

The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Three alternative assay approaches are presented for implementing MTS analysis of chromosome 21, providing a 10-fold concentration enhancement while preserving assay precision. PMID:24854517

Methods for multiplex template sampling in digital PCR assays.

PubMed

Petriv, Oleh I; Heyries, Kevin A; VanInsberghe, Michael; Walker, David; Hansen, Carl L

2014-01-01

The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Three alternative assay approaches are presented for implementing MTS analysis of chromosome 21, providing a 10-fold concentration enhancement while preserving assay precision.

Multiwavelength digital holography with wavelength-multiplexed holograms and arbitrary symmetric phase shifts.

PubMed

Tahara, Tatsuki; Otani, Reo; Omae, Kaito; Gotohda, Takuya; Arai, Yasuhiko; Takaki, Yasuhiro

2017-05-15

We propose multiwavelength in-line digital holography with wavelength-multiplexed phase-shifted holograms and arbitrary symmetric phase shifts. We use phase-shifting interferometry selectively extracting wavelength information to reconstruct multiwavelength object waves separately from wavelength-multiplexed monochromatic images. The proposed technique obtains systems of equations for real and imaginary parts of multiwavelength object waves from the holograms by introducing arbitrary symmetric phase shifts. Then, the technique derives each complex amplitude distribution of each object wave selectively and analytically by solving the two systems of equations. We formulate the algorithm in the case of an arbitrary number of wavelengths and confirm its validity numerically and experimentally in the cases where the number of wavelengths is two and three.

A time domain based method for the accurate measurement of Q-factor and resonance frequency of microwave resonators

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gyüre, B.; Márkus, B. G.; Bernáth, B.

2015-09-15

We present a novel method to determine the resonant frequency and quality factor of microwave resonators which is faster, more stable, and conceptually simpler than the yet existing techniques. The microwave resonator is pumped with the microwave radiation at a frequency away from its resonance. It then emits an exponentially decaying radiation at its eigen-frequency when the excitation is rapidly switched off. The emitted microwave signal is down-converted with a microwave mixer, digitized, and its Fourier transformation (FT) directly yields the resonance curve in a single shot. Being a FT based method, this technique possesses the Fellgett (multiplex) and Connesmore » (accuracy) advantages and it conceptually mimics that of pulsed nuclear magnetic resonance. We also establish a novel benchmark to compare accuracy of the different approaches of microwave resonator measurements. This shows that the present method has similar accuracy to the existing ones, which are based on sweeping or modulating the frequency of the microwave radiation.« less

Simultaneous single-shot readout of multi-qubit circuits using a traveling-wave parametric amplifier

NASA Astrophysics Data System (ADS)

O'Brien, Kevin

Observing and controlling the state of ever larger quantum systems is critical for advancing quantum computation. Utilizing a Josephson traveling wave parametric amplifier (JTWPA), we demonstrate simultaneous multiplexed single shot readout of 10 transmon qubits in a planar architecture. We employ digital image sideband rejection to eliminate noise at the image frequencies. We quantify crosstalk and infidelity due to simultaneous readout and control of multiple qubits. Based on current amplifier technology, this approach can scale to simultaneous readout of at least 20 qubits. This work was supported by the Army Research Office.

Multiplexing of Radio-Frequency Single Electron Transistors

NASA Technical Reports Server (NTRS)

Stevenson, Thomas R.; Pellerano, F. A.; Stahle, C. M.; Aidala, K.; Schoelkopf, R. J.; Krebs, Carolyn (Technical Monitor)

2001-01-01

We present results on wavelength division multiplexing of radio-frequency single electron transistors. We use a network of resonant impedance matching circuits to direct applied rf carrier waves to different transistors depending on carrier frequency. A two-channel demonstration of this concept using discrete components successfully reconstructed input signals with small levels of cross coupling. A lithographic version of the rf circuits had measured parameters in agreement with electromagnetic modeling, with reduced cross capacitance and inductance, and should allow 20 to 50 channels to be multiplexed.

Multi-channel detector readout method and integrated circuit

DOEpatents

Moses, William W.; Beuville, Eric; Pedrali-Noy, Marzio

2006-12-12

An integrated circuit which provides multi-channel detector readout from a detector array. The circuit receives multiple signals from the elements of a detector array and compares the sampled amplitudes of these signals against a noise-floor threshold and against one another. A digital signal is generated which corresponds to the location of the highest of these signal amplitudes which exceeds the noise floor threshold. The digital signal is received by a multiplexing circuit which outputs an analog signal corresponding the highest of the input signal amplitudes. In addition a digital control section provides for programmatic control of the multiplexer circuit, amplifier gain, amplifier reset, masking selection, and test circuit functionality on each input thereof.

Multi-channel detector readout method and integrated circuit

DOEpatents

Moses, William W.; Beuville, Eric; Pedrali-Noy, Marzio

2004-05-18

An integrated circuit which provides multi-channel detector readout from a detector array. The circuit receives multiple signals from the elements of a detector array and compares the sampled amplitudes of these signals against a noise-floor threshold and against one another. A digital signal is generated which corresponds to the location of the highest of these signal amplitudes which exceeds the noise floor threshold. The digital signal is received by a multiplexing circuit which outputs an analog signal corresponding the highest of the input signal amplitudes. In addition a digital control section provides for programmatic control of the multiplexer circuit, amplifier gain, amplifier reset, masking selection, and test circuit functionality on each input thereof.

The LiteBIRD Satellite Mission: Sub-Kelvin Instrument

NASA Astrophysics Data System (ADS)

Suzuki, A.; Ade, P. A. R.; Akiba, Y.; Alonso, D.; Arnold, K.; Aumont, J.; Baccigalupi, C.; Barron, D.; Basak, S.; Beckman, S.; Borrill, J.; Boulanger, F.; Bucher, M.; Calabrese, E.; Chinone, Y.; Cho, S.; Crill, B.; Cukierman, A.; Curtis, D. W.; de Haan, T.; Dobbs, M.; Dominjon, A.; Dotani, T.; Duband, L.; Ducout, A.; Dunkley, J.; Duval, J. M.; Elleflot, T.; Eriksen, H. K.; Errard, J.; Fischer, J.; Fujino, T.; Funaki, T.; Fuskeland, U.; Ganga, K.; Goeckner-Wald, N.; Grain, J.; Halverson, N. W.; Hamada, T.; Hasebe, T.; Hasegawa, M.; Hattori, K.; Hattori, M.; Hayes, L.; Hazumi, M.; Hidehira, N.; Hill, C. A.; Hilton, G.; Hubmayr, J.; Ichiki, K.; Iida, T.; Imada, H.; Inoue, M.; Inoue, Y.; Irwin, K. D.; Ishino, H.; Jeong, O.; Kanai, H.; Kaneko, D.; Kashima, S.; Katayama, N.; Kawasaki, T.; Kernasovskiy, S. A.; Keskitalo, R.; Kibayashi, A.; Kida, Y.; Kimura, K.; Kisner, T.; Kohri, K.; Komatsu, E.; Komatsu, K.; Kuo, C. L.; Kurinsky, N. A.; Kusaka, A.; Lazarian, A.; Lee, A. T.; Li, D.; Linder, E.; Maffei, B.; Mangilli, A.; Maki, M.; Matsumura, T.; Matsuura, S.; Meilhan, D.; Mima, S.; Minami, Y.; Mitsuda, K.; Montier, L.; Nagai, M.; Nagasaki, T.; Nagata, R.; Nakajima, M.; Nakamura, S.; Namikawa, T.; Naruse, M.; Nishino, H.; Nitta, T.; Noguchi, T.; Ogawa, H.; Oguri, S.; Okada, N.; Okamoto, A.; Okamura, T.; Otani, C.; Patanchon, G.; Pisano, G.; Rebeiz, G.; Remazeilles, M.; Richards, P. L.; Sakai, S.; Sakurai, Y.; Sato, Y.; Sato, N.; Sawada, M.; Segawa, Y.; Sekimoto, Y.; Seljak, U.; Sherwin, B. D.; Shimizu, T.; Shinozaki, K.; Stompor, R.; Sugai, H.; Sugita, H.; Suzuki, J.; Tajima, O.; Takada, S.; Takaku, R.; Takakura, S.; Takatori, S.; Tanabe, D.; Taylor, E.; Thompson, K. L.; Thorne, B.; Tomaru, T.; Tomida, T.; Tomita, N.; Tristram, M.; Tucker, C.; Turin, P.; Tsujimoto, M.; Uozumi, S.; Utsunomiya, S.; Uzawa, Y.; Vansyngel, F.; Wehus, I. K.; Westbrook, B.; Willer, M.; Whitehorn, N.; Yamada, Y.; Yamamoto, R.; Yamasaki, N.; Yamashita, T.; Yoshida, M.

2018-05-01

Inflation is the leading theory of the first instant of the universe. Inflation, which postulates that the universe underwent a period of rapid expansion an instant after its birth, provides convincing explanation for cosmological observations. Recent advancements in detector technology have opened opportunities to explore primordial gravitational waves generated by the inflation through "B-mode" (divergent-free) polarization pattern embedded in the cosmic microwave background anisotropies. If detected, these signals would provide strong evidence for inflation, point to the correct model for inflation, and open a window to physics at ultra-high energies. LiteBIRD is a satellite mission with a goal of detecting degree-and-larger-angular-scale B-mode polarization. LiteBIRD will observe at the second Lagrange point with a 400 mm diameter telescope and 2622 detectors. It will survey the entire sky with 15 frequency bands from 40 to 400 GHz to measure and subtract foregrounds. The US LiteBIRD team is proposing to deliver sub-Kelvin instruments that include detectors and readout electronics. A lenslet-coupled sinuous antenna array will cover low-frequency bands (40-235 GHz) with four frequency arrangements of trichroic pixels. An orthomode-transducer-coupled corrugated horn array will cover high-frequency bands (280-402 GHz) with three types of single frequency detectors. The detectors will be made with transition edge sensor (TES) bolometers cooled to a 100 milli-Kelvin base temperature by an adiabatic demagnetization refrigerator. The TES bolometers will be read out using digital frequency multiplexing with Superconducting QUantum Interference Device (SQUID) amplifiers. Up to 78 bolometers will be multiplexed with a single SQUID amplifier. We report on the sub-Kelvin instrument design and ongoing developments for the LiteBIRD mission.

Large object investigation by digital holography with effective spectrum multiplexing under single-exposure approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Ning, E-mail: coolboy006@sohu.com; Zhang, Yingying; Xie, Jun

2014-10-13

We present a method to investigate large object by digital holography with effective spectrum multiplexing under single-exposure approach. This method splits the original reference beam and redirects one of its branches as a second object beam. Through the modified Mach-Zehnder interferometer, the two object beams can illuminate different parts of the large object and create a spectrum multiplexed hologram onto the focal plane array of the charge-coupled device/complementary metal oxide semiconductor camera. After correct spectrum extraction and image reconstruction, the large object can be fully observed within only one single snap-shot. The flexibility and great performance make our method amore » very attractive and promising technique for large object investigation under common 632.8 nm illumination.« less

On-field measurement trial of 4×128 Gbps PDM-QPSK signals by linear optical sampling

NASA Astrophysics Data System (ADS)

Bin Liu; Wu, Zhichao; Fu, Songnian; Feng, Yonghua; Liu, Deming

2017-02-01

Linear optical sampling is a promising characterization technique for advanced modulation formats, together with digital signal processing (DSP) and software-synchronized algorithm. We theoretically investigate the acquisition of optical sampling, when the high-speed signal under test is either periodic or random. Especially, when the profile of optical sampling pulse is asymmetrical, the repetition frequency of sampling pulse needs careful adjustment in order to obtain correct waveform. Then, we demonstrate on-field measurement trial of commercial four-channel 128 Gbps polarization division multiplexing quadrature phase shift keying (PDM-QPSK) signals with truly random characteristics by self-developed equipment. A passively mode-locked fiber laser (PMFL) with a repetition frequency of 95.984 MHz is used as optical sampling source, meanwhile four balanced photo detectors (BPDs) with 400 MHz bandwidth and four-channel analog-to-digital convertor (ADC) with 1.25 GS/s sampling rate are used for data acquisition. The performance comparison with conventional optical modulation analyzer (OMA) verifies that the self-developed equipment has the advantages of low cost, easy implementation, and fast response.

Shuttle S-band communications technical concepts

NASA Technical Reports Server (NTRS)

Seyl, J. W.; Seibert, W. W.; Porter, J. A.; Eggers, D. S.; Novosad, S. W.; Vang, H. A.; Lenett, S. D.; Lewton, W. A.; Pawlowski, J. F.

1985-01-01

Using the S-band communications system, shuttle orbiter can communicate directly with the Earth via the Ground Spaceflight Tracking and Data Network (GSTDN) or via the Tracking and Data Relay Satellite System (TDRSS). The S-band frequencies provide the primary links for direct Earth and TDRSS communications during all launch and entry/landing phases of shuttle missions. On orbit, S-band links are used when TDRSS Ku-band is not available, when conditions require orbiter attitudes unfavorable to Ku-band communications, or when the payload bay doors are closed. the S-band communications functional requirements, the orbiter hardware configuration, and the NASA S-band communications network are described. The requirements and implementation concepts which resulted in techniques for shuttle S-band hardware development discussed include: (1) digital voice delta modulation; (2) convolutional coding/Viterbi decoding; (3) critical modulation index for phase modulation using a Costas loop (phase-shift keying) receiver; (4) optimum digital data modulation parameters for continuous-wave frequency modulation; (5) intermodulation effects of subcarrier ranging and time-division multiplexing data channels; (6) radiofrequency coverage; and (7) despreading techniques under poor signal-to-noise conditions. Channel performance is reviewed.

Fault tolerance in space-based digital signal processing and switching systems: Protecting up-link processing resources, demultiplexer, demodulator, and decoder

NASA Technical Reports Server (NTRS)

Redinbo, Robert

1994-01-01

Fault tolerance features in the first three major subsystems appearing in the next generation of communications satellites are described. These satellites will contain extensive but efficient high-speed processing and switching capabilities to support the low signal strengths associated with very small aperture terminals. The terminals' numerous data channels are combined through frequency division multiplexing (FDM) on the up-links and are protected individually by forward error-correcting (FEC) binary convolutional codes. The front-end processing resources, demultiplexer, demodulators, and FEC decoders extract all data channels which are then switched individually, multiplexed, and remodulated before retransmission to earth terminals through narrow beam spot antennas. Algorithm based fault tolerance (ABFT) techniques, which relate real number parity values with data flows and operations, are used to protect the data processing operations. The additional checking features utilize resources that can be substituted for normal processing elements when resource reconfiguration is required to replace a failed unit.

Optical-wireless-optical full link for polarization multiplexing quadrature amplitude/phase modulation signal transmission.

PubMed

Li, Xinying; Yu, Jianjun; Chi, Nan; Zhang, Junwen

2013-11-15

We propose and experimentally demonstrate an optical wireless integration system at the Q-band, in which up to 40 Gb/s polarization multiplexing multilevel quadrature amplitude/phase modulation (PM-QAM) signal can be first transmitted over 20 km single-mode fiber-28 (SMF-28), then delivered over a 2 m 2 × 2 multiple-input multiple-output wireless link, and finally transmitted over another 20 km SMF-28. The PM-QAM modulated wireless millimeter-wave (mm-wave) signal at 40 GHz is generated based on the remote heterodyning technique, and demodulated by the radio-frequency transparent photonic technique based on homodyne coherent detection and baseband digital signal processing. The classic constant modulus algorithm equalization is used at the receiver to realize polarization demultiplexing of the PM-QAM signal. For the first time, to the best of our knowledge, we realize the conversion of the PM-QAM modulated wireless mm-wave signal to the optical signal as well as 20 km fiber transmission of the converted optical signal.

Clinical Utility of Circulating Tumor DNA for Molecular Assessment and Precision Medicine in Pancreatic Cancer.

PubMed

Takai, Erina; Totoki, Yasushi; Nakamura, Hiromi; Kato, Mamoru; Shibata, Tatsuhiro; Yachida, Shinichi

2016-01-01

Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. The genomic landscape of the PDAC genome features four frequently mutated genes (KRAS, CDKN2A, TP53, and SMAD4) and dozens of candidate driver genes altered at low frequency, including potential clinical targets. Circulating cell-free DNA (cfDNA) is a promising resource to detect molecular characteristics of tumors, supporting the concept of "liquid biopsy".We determined the mutational status of KRAS in plasma cfDNA using multiplex droplet digital PCR in 259 patients with PDAC, retrospectively. Furthermore, we constructed a novel modified SureSelect-KAPA-Illumina platform and an original panel of 60 genes. We then performed targeted deep sequencing of cfDNA in 48 patients who had ≥1 % mutant allele frequencies of KRAS in plasma cfDNA.Droplet digital PCR detected KRAS mutations in plasma cfDNA in 63 of 107 (58.9 %) patients with inoperable tumors. Importantly, potentially targetable somatic mutations were identified in 14 of 48 patients (29.2 %) examined by cfDNA sequencing.Our two-step approach with plasma cfDNA, combining droplet digital PCR and targeted deep sequencing, is a feasible clinical approach. Assessment of mutations in plasma cfDNA may provide a new diagnostic tool, assisting decisions for optimal therapeutic strategies for PDAC patients.

Opportunistic Access in Frequency Hopping Cognitive Radio Networks

DTIC Science & Technology

2014-03-27

thresholding MA multiple access MFSK M-ary frequency shift keying MIMO multiple-input/multiple-output OFDM orthogonal frequency-division multiplexing x...adaptive BER performance as a function of ISR with orthogonal frequency-division multiplexing ( OFDM ) interference present. . . . . . . . . . 41 4.15 Non...adaptive BER performance as a function of EB/N0 with OFDM interfer- ence present

Multiplex quantification of 12 European Union authorized genetically modified maize lines with droplet digital polymerase chain reaction.

PubMed

Dobnik, David; Spilsberg, Bjørn; Bogožalec Košir, Alexandra; Holst-Jensen, Arne; Žel, Jana

2015-08-18

Presence of genetically modified organisms (GMO) in food and feed products is regulated in many countries. The European Union (EU) has implemented a threshold for labeling of products containing more than 0.9% of authorized GMOs per ingredient. As the number of GMOs has increased over time, standard-curve based simplex quantitative polymerase chain reaction (qPCR) analyses are no longer sufficiently cost-effective, despite widespread use of initial PCR based screenings. Newly developed GMO detection methods, also multiplex methods, are mostly focused on screening and detection but not quantification. On the basis of droplet digital PCR (ddPCR) technology, multiplex assays for quantification of all 12 EU authorized GM maize lines (per April first 2015) were developed. Because of high sequence similarity of some of the 12 GM targets, two separate multiplex assays were needed. In both assays (4-plex and 10-plex), the transgenes were labeled with one fluorescence reporter and the endogene with another (GMO concentration = transgene/endogene ratio). It was shown that both multiplex assays produce specific results and that performance parameters such as limit of quantification, repeatability, and trueness comply with international recommendations for GMO quantification methods. Moreover, for samples containing GMOs, the throughput and cost-effectiveness is significantly improved compared to qPCR. Thus, it was concluded that the multiplex ddPCR assays could be applied for routine quantification of 12 EU authorized GM maize lines. In case of new authorizations, the events can easily be added to the existing multiplex assays. The presented principle of quantitative multiplexing can be applied to any other domain.

Results of a remote multiplexer/digitizer unit accuracy and environmental study

NASA Technical Reports Server (NTRS)

Wilner, D. O.

1977-01-01

A remote multiplexer/digitizer unit (RMDU), a part of the airborne integrated flight test data system, was subjected to an accuracy study. The study was designed to show the effects of temperature, altitude, and vibration on the RMDU. The RMDU was subjected to tests at temperatures from -54 C (-65 F) to 71 C (160 F), and the resulting data are presented here, along with a complete analysis of the effects. The methods and means used for obtaining correctable data and correcting the data are also discussed.

Dual-wavelength phase-shifting digital holography selectively extracting wavelength information from wavelength-multiplexed holograms.

PubMed

Tahara, Tatsuki; Mori, Ryota; Kikunaga, Shuhei; Arai, Yasuhiko; Takaki, Yasuhiro

2015-06-15

Dual-wavelength phase-shifting digital holography that selectively extracts wavelength information from five wavelength-multiplexed holograms is presented. Specific phase shifts for respective wavelengths are introduced to remove the crosstalk components and extract only the object wave at the desired wavelength from the holograms. Object waves in multiple wavelengths are selectively extracted by utilizing 2π ambiguity and the subtraction procedures based on phase-shifting interferometry. Numerical results show the validity of the proposed technique. The proposed technique is also experimentally demonstrated.

Centralized light-source optical access network based on polarization multiplexing.

PubMed

Grassi, Fulvio; Mora, José; Ortega, Beatriz; Capmany, José

2010-03-01

This paper presents and demonstrates a centralized light source optical access network based on optical polarization multiplexing technique. By using two optical sources emitting light orthogonally polarized in the Central Node for downstream and upstream operations, the Remote Node is kept source-free. EVM values below telecommunication standard requirements have been measured experimentally when bidirectional digital signals have been transmitted over 10 km of SMF employing subcarrier multiplexing technique in the electrical domain.

Dual-polarization multi-band optical OFDM transmission and transceiver limitations for up to 500 Gb/s uncompensated long-haul links.

PubMed

Giacoumidis, E; Jarajreh, M A; Sygletos, S; Le, S T; Farjady, F; Tsokanos, A; Hamié, A; Pincemin, E; Jaouën, Y; Ellis, A D; Doran, N J

2014-05-05

A number of critical issues for dual-polarization single- and multi-band optical orthogonal-frequency division multiplexing (DP-SB/MB-OFDM) signals are analyzed in dispersion compensation fiber (DCF)-free long-haul links. For the first time, different DP crosstalk removal techniques are compared, the maximum transmission-reach is investigated, and the impact of subcarrier number and high-level modulation formats are explored thoroughly. It is shown, for a bit-error-rate (BER) of 10(-3), 2000 km of quaternary phase-shift keying (QPSK) DP-MB-OFDM transmission is feasible. At high launched optical powers (LOP), maximum-likelihood decoding can extend the LOP of 40 Gb/s QPSK DP-SB-OFDM at 2000 km by 1.5 dB compared to zero-forcing. For a 100 Gb/s DP-MB-OFDM system, a high number of subcarriers contribute to improved BER but at the cost of digital signal processing computational complexity, whilst by adapting the cyclic prefix length the BER can be improved for a low number of subcarriers. In addition, when 16-quadrature amplitude modulation (16QAM) is employed the digital-to-analogue/analogue-to-digital converter (DAC/ADC) bandwidth is relaxed with a degraded BER; while the 'circular' 8QAM is slightly superior to its 'rectangular' form. Finally, the transmission of wavelength-division multiplexing DP-MB-OFDM and single-carrier DP-QPSK is experimentally compared for up to 500 Gb/s showing great potential and similar performance at 1000 km DCF-free G.652 line.

Frequency-Domain Streak Camera and Tomography for Ultrafast Imaging of Evolving and Channeled Plasma Accelerator Structures

NASA Astrophysics Data System (ADS)

Li, Zhengyan; Zgadzaj, Rafal; Wang, Xiaoming; Reed, Stephen; Dong, Peng; Downer, Michael C.

2010-11-01

We demonstrate a prototype Frequency Domain Streak Camera (FDSC) that can capture the picosecond time evolution of the plasma accelerator structure in a single shot. In our prototype Frequency-Domain Streak Camera, a probe pulse propagates obliquely to a sub-picosecond pump pulse that creates an evolving nonlinear index "bubble" in fused silica glass, supplementing a conventional Frequency Domain Holographic (FDH) probe-reference pair that co-propagates with the "bubble". Frequency Domain Tomography (FDT) generalizes Frequency-Domain Streak Camera by probing the "bubble" from multiple angles and reconstructing its morphology and evolution using algorithms similar to those used in medical CAT scans. Multiplexing methods (Temporal Multiplexing and Angular Multiplexing) improve data storage and processing capability, demonstrating a compact Frequency Domain Tomography system with a single spectrometer.

Time Division Multiplexing of Semiconductor Qubits

NASA Astrophysics Data System (ADS)

Jarratt, Marie Claire; Hornibrook, John; Croot, Xanthe; Watson, John; Gardner, Geoff; Fallahi, Saeed; Manfra, Michael; Reilly, David

Readout chains, comprising resonators, amplifiers, and demodulators, are likely to be precious resources in quantum computing architectures. The potential to share readout resources is contingent on realising efficient means of time-division multiplexing (TDM) schemes that are compatible with quantum computing. Here, we demonstrate TDM using a GaAs quantum dot device with multiple charge sensors. Our device incorporates chip-level switches that do not load the impedance matching network. When used in conjunction with frequency multiplexing, each frequency tone addresses multiple time-multiplexed qubits, vastly increasing the capacity of a single readout line.

Analysis of the Effects of Phase Noise and Frequency Offset in Orthogonal Frequency Division Multiplexing (OFDM) Systems

DTIC Science & Technology

2004-03-01

Data Communication , http://www.iec.org/, last accessed December 2003. 13. Klaus Witrisal, “Orthogonal Frequency Division Multiplexing (OFDM) for...http://ieeexplore.ieee.org, last accessed 26 February 2003. 12. The International Engineering Consortium, Web Forum Tutorials, OFDM for Mobile

Wavelength Division Multiplexing Scheme for Radio-Frequency Single Electron Transistors

NASA Technical Reports Server (NTRS)

Stevenson, Thomas R.; Pellerano, F. A.; Stahle, C. M.; Aidala, K.; Schoelkopf, R. J.; Krebs, Carolyn (Technical Monitor)

2001-01-01

We describe work on a wavelength division multiplexing scheme for radio-frequency single electron transistors. We use a network of resonant impedance matching circuits to direct applied rf carrier waves to different transistors depending on carrier frequency. Using discrete components, we made a two-channel demonstration of this concept and successfully reconstructed input signals with small levels of cross coupling. A lithographic version of the rf circuits had measured parameters in agreement with electromagnetic modeling, with reduced cross capacitance and inductance, and should allow 20 to 50 channels to be multiplexed.

Preface to the special issue on ;Optical Communications Exploiting the Space Domain;

NASA Astrophysics Data System (ADS)

Wang, Jian; Yu, Siyuan; Li, Guifang

2018-02-01

The demand for high capacity optical communications will continue to be driven by the exponential growth of global internet traffic. Optical communications are about the exploitation of different physical dimensions of light waves, including complex amplitude, frequency (or wavelength), time, polarization, etc. Conventional techniques such as wavelength-division multiplexing (WDM), time-division multiplexing (TDM) and polarization-division multiplexing (PDM) have almost reached their scalability limits. Space domain is the only known physical dimension left and space-division multiplexing (SDM) seems the only option to further scale the transmission capacity and spectral efficiency of optical communications. In recent years, few-mode fiber (FMF), multi-mode fiber (MMF), multi-core fiber (MCF) and few-mode multi-core fiber (FM-MCF) have been widely explored as promising candidates for fiber-based SDM. The challenges for SDM include efficient (de)multiplexer, amplifiers, and multiple-input multiple-output (MIMO) digital signal processing (DSP) techniques. Photonic integration will also be a key technology to SDM. Meanwhile, free-space and underwater optical communications have also exploited the space domain to increase the transmission capacity and spectral efficiency. The challenges include long-distance transmission limited by propagation loss, divergence, scattering and turbulence. Very recently, helically phased light beams carrying orbital angular momentum (OAM) have also seen potential applications both in free-space, underwater and fiber-based optical communications. Actually, different mode bases such as linearly polarized (LP) modes and OAM modes can be employed for SDM. Additionally, SDM could be used in chip-scale photonic interconnects and data center optical interconnects. Quantum processing exploiting the space domain is of great interest. The information capacity limit and physical layer security in SDM optical communications systems are important issues to be addressed.

Optimized and secure technique for multiplexing QR code images of single characters: application to noiseless messages retrieval

NASA Astrophysics Data System (ADS)

Trejos, Sorayda; Fredy Barrera, John; Torroba, Roberto

2015-08-01

We present for the first time an optical encrypting-decrypting protocol for recovering messages without speckle noise. This is a digital holographic technique using a 2f scheme to process QR codes entries. In the procedure, letters used to compose eventual messages are individually converted into a QR code, and then each QR code is divided into portions. Through a holographic technique, we store each processed portion. After filtering and repositioning, we add all processed data to create a single pack, thus simplifying the handling and recovery of multiple QR code images, representing the first multiplexing procedure applied to processed QR codes. All QR codes are recovered in a single step and in the same plane, showing neither cross-talk nor noise problems as in other methods. Experiments have been conducted using an interferometric configuration and comparisons between unprocessed and recovered QR codes have been performed, showing differences between them due to the involved processing. Recovered QR codes can be successfully scanned, thanks to their noise tolerance. Finally, the appropriate sequence in the scanning of the recovered QR codes brings a noiseless retrieved message. Additionally, to procure maximum security, the multiplexed pack could be multiplied by a digital diffuser as to encrypt it. The encrypted pack is easily decoded by multiplying the multiplexing with the complex conjugate of the diffuser. As it is a digital operation, no noise is added. Therefore, this technique is threefold robust, involving multiplexing, encryption, and the need of a sequence to retrieve the outcome.

Method and apparatus for high speed data acquisition and processing

DOEpatents

Ferron, J.R.

1997-02-11

A method and apparatus are disclosed for high speed digital data acquisition. The apparatus includes one or more multiplexers for receiving multiple channels of digital data at a low data rate and asserting a multiplexed data stream at a high data rate, and one or more FIFO memories for receiving data from the multiplexers and asserting the data to a real time processor. Preferably, the invention includes two multiplexers, two FIFO memories, and a 64-bit bus connecting the FIFO memories with the processor. Each multiplexer receives four channels of 14-bit digital data at a rate of up to 5 MHz per channel, and outputs a data stream to one of the FIFO memories at a rate of 20 MHz. The FIFO memories assert output data in parallel to the 64-bit bus, thus transferring 14-bit data values to the processor at a combined rate of 40 MHz. The real time processor is preferably a floating-point processor which processes 32-bit floating-point words. A set of mask bits is prestored in each 32-bit storage location of the processor memory into which a 14-bit data value is to be written. After data transfer from the FIFO memories, mask bits are concatenated with each stored 14-bit data value to define a valid 32-bit floating-point word. Preferably, a user can select any of several modes for starting and stopping direct memory transfers of data from the FIFO memories to memory within the real time processor, by setting the content of a control and status register. 15 figs.

Method and apparatus for high speed data acquisition and processing

DOEpatents

Ferron, John R.

1997-01-01

A method and apparatus for high speed digital data acquisition. The apparatus includes one or more multiplexers for receiving multiple channels of digital data at a low data rate and asserting a multiplexed data stream at a high data rate, and one or more FIFO memories for receiving data from the multiplexers and asserting the data to a real time processor. Preferably, the invention includes two multiplexers, two FIFO memories, and a 64-bit bus connecting the FIFO memories with the processor. Each multiplexer receives four channels of 14-bit digital data at a rate of up to 5 MHz per channel, and outputs a data stream to one of the FIFO memories at a rate of 20 MHz. The FIFO memories assert output data in parallel to the 64-bit bus, thus transferring 14-bit data values to the processor at a combined rate of 40 MHz. The real time processor is preferably a floating-point processor which processes 32-bit floating-point words. A set of mask bits is prestored in each 32-bit storage location of the processor memory into which a 14-bit data value is to be written. After data transfer from the FIFO memories, mask bits are concatenated with each stored 14-bit data value to define a valid 32-bit floating-point word. Preferably, a user can select any of several modes for starting and stopping direct memory transfers of data from the FIFO memories to memory within the real time processor, by setting the content of a control and status register.

Frequency-Domain Streak Camera and Tomography for Ultrafast Imaging of Evolving and Channeled Plasma Accelerator Structures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li Zhengyan; Zgadzaj, Rafal; Wang Xiaoming

2010-11-04

We demonstrate a prototype Frequency Domain Streak Camera (FDSC) that can capture the picosecond time evolution of the plasma accelerator structure in a single shot. In our prototype Frequency-Domain Streak Camera, a probe pulse propagates obliquely to a sub-picosecond pump pulse that creates an evolving nonlinear index 'bubble' in fused silica glass, supplementing a conventional Frequency Domain Holographic (FDH) probe-reference pair that co-propagates with the 'bubble'. Frequency Domain Tomography (FDT) generalizes Frequency-Domain Streak Camera by probing the 'bubble' from multiple angles and reconstructing its morphology and evolution using algorithms similar to those used in medical CAT scans. Multiplexing methods (Temporalmore » Multiplexing and Angular Multiplexing) improve data storage and processing capability, demonstrating a compact Frequency Domain Tomography system with a single spectrometer.« less

Fundamentals of multiplexing with digital PCR.

PubMed

Whale, Alexandra S; Huggett, Jim F; Tzonev, Svilen

2016-12-01

Over the past decade numerous publications have demonstrated how digital PCR (dPCR) enables precise and sensitive quantification of nucleic acids in a wide range of applications in both healthcare and environmental analysis. This has occurred in parallel with the advances in partitioning fluidics that enable a reaction to be subdivided into an increasing number of partitions. As the majority of dPCR systems are based on detection in two discrete optical channels, most research to date has focused on quantification of one or two targets within a single reaction. Here we describe 'higher order multiplexing' that is the unique ability of dPCR to precisely measure more than two targets in the same reaction. Using examples, we describe the different types of duplex and multiplex reactions that can be achieved. We also describe essential experimental considerations to ensure accurate quantification of multiple targets.

Atmospheric free-space coherent optical communications with adaptive optics

NASA Astrophysics Data System (ADS)

Ting, Chueh; Zhang, Chengyu; Yang, Zikai

2017-02-01

Free-space coherent optical communications have a potential application to offer last mile bottleneck solution in future local area networks (LAN) because of their information carrier, information security and license-free status. Coherent optical communication systems using orthogonal frequency division multiplexing (OFDM) digital modulation are successfully demonstrated in a long-haul tens Giga bits via optical fiber, but they are not yet available in free space due to atmospheric turbulence-induced channel fading. Adaptive optics is recognized as a promising technology to mitigate the effects of atmospheric turbulence in free-space optics. In this paper, a free-space coherent optical communication system using an OFDM digital modulation scheme and adaptive optics (FSO OFDM AO) is proposed, a Gamma-Gamma distribution statistical channel fading model for the FSO OFDM AO system is examined, and FSO OFDM AO system performance is evaluated in terms of bit error rate (BER) versus various propagation distances.

Digital chaos-masked optical encryption scheme enhanced by two-dimensional key space

NASA Astrophysics Data System (ADS)

Liu, Ling; Xiao, Shilin; Zhang, Lu; Bi, Meihua; Zhang, Yunhao; Fang, Jiafei; Hu, Weisheng

2017-09-01

A digital chaos-masked optical encryption scheme is proposed and demonstrated. The transmitted signal is completely masked by interference chaotic noise in both bandwidth and amplitude with analog method via dual-drive Mach-Zehnder modulator (DDMZM), making the encrypted signal analog, noise-like and unrecoverable by post-processing techniques. The decryption process requires precise matches of both the amplitude and phase between the cancellation and interference chaotic noises, which provide a large two-dimensional key space with the help of optical interference cancellation technology. For 10-Gb/s 16-quadrature amplitude modulation (QAM) orthogonal frequency division multiplexing (OFDM) signal over the maximum transmission distance of 80 km without dispersion compensation or inline amplifier, the tolerable mismatch ranges of amplitude and phase/delay at the forward error correction (FEC) threshold of 3.8×10-3 are 0.44 dB and 0.08 ns respectively.

Multiplex newborn screening for Pompe, Fabry, Hunter, Gaucher, and Hurler diseases using a digital microfluidic platform.

PubMed

Sista, Ramakrishna S; Wang, Tong; Wu, Ning; Graham, Carrie; Eckhardt, Allen; Winger, Theodore; Srinivasan, Vijay; Bali, Deeksha; Millington, David S; Pamula, Vamsee K

2013-09-23

New therapies for lysosomal storage diseases (LSDs) have generated interest in screening newborns for these conditions. We present performance validation data on a digital microfluidic platform that performs multiplex enzymatic assays for Pompe, Fabry, Hunter, Gaucher, and Hurler diseases. We developed an investigational disposable digital microfluidic cartridge that uses a single dried blood spot (DBS) punch for performing a 5-plex fluorometric enzymatic assay on up to 44 DBS samples. Precision and linearity of the assays were determined by analyzing quality control DBS samples; clinical performance was determined by analyzing 600 presumed normal and known affected samples (12 for Pompe, 7 for Fabry and 10 each for Hunter, Gaucher and Hurler). Overall coefficient of variation (CV) values between cartridges, days, instruments, and operators ranged from 2 to 21%; linearity correlation coefficients were ≥0.98 for all assays. The multiplex enzymatic assay performed from a single DBS punch was able to discriminate presumed normal from known affected samples for 5 LSDs. Digital microfluidic technology shows potential for rapid, high-throughput screening for 5 LSDs in a newborn screening laboratory environment. Sample preparation to enzymatic activity on each cartridge is less than 3h. Copyright © 2013 Elsevier B.V. All rights reserved.

Multiplexed microimmunoassays on a digital versatile disk.

PubMed

Morais, Sergi; Tortajada-Genaro, Luis A; Arnandis-Chover, Tania; Puchades, Rosa; Maquieira, Angel

2009-07-15

Multiplexed microimmunoassays for five critical compounds were developed using a digital versatile disk (DVD) as an analytical support and detecting technology. To this end, coating conjugates were adsorbed on the polycarbonate face of the disk; a pool of specific antibodies, gold labeled secondary antibodies, and silver amplification were addressed for developing the assays. The detection principle is based on the capture of attenuated analog signals with the disk drive that were proportional to optical density of the immunoreaction product. The multiplexed assay achieved detection limits (IC10) of 0.06, 0.25, 0.37, 0.16, and 0.10 microg/L, sensitivities of (IC50) 0.54, 1.54, 2.62, 2.02, and 5.9 microg/L, and dynamic ranges of 2 orders of magnitude for atrazine, chlorpyrifos, metolachlor, sulfathiazole, and tetracycline, respectively. The features of the methodology were verified by analyzing natural waters and compared with reference chromatographic methods, showing its potential for high-throughput multiplexed screening applications. Analytes of different chemical nature (pesticides and antibiotics) were directly quantified without sample treatment or preconcentration in a total time of 30 min with similar sensitivity and selectivity to the ELISA plate format using the same immunoreagents. The multianalyte capabilities of immunoassaying methods developed with digital disk and drive demonstrated the competitiveness to quantify targets that require different sample treatment and instrumentation by chromatographic methods.

Comparisons between Common and Dedicated Reference Signals for MIMO Multiplexing Using Precoding in Evolved UTRA Downlink

NASA Astrophysics Data System (ADS)

Taoka, Hidekazu; Kishiyama, Yoshihisa; Higuchi, Kenichi; Sawahashi, Mamoru

This paper presents comparisons between common and dedicated reference signals (RSs) for channel estimation in MIMO multiplexing using codebook-based precoding for orthogonal frequency division multiplexing (OFDM) radio access in the Evolved UTRA downlink with frequency division duplexing (FDD). We clarify the best RS structure for precoding-based MIMO multiplexing based on comparisons of the structures in terms of the achievable throughput taking into account the overhead of the common and dedicated RSs and the precoding matrix indication (PMI) signal. Based on extensive simulations on the throughput in 2-by-2 and 4-by-4 MIMO multiplexing with precoding, we clarify that channel estimation based on common RSs multiplied with the precoding matrix indicated by the PMI signal achieves higher throughput compared to that using dedicated RSs irrespective of the number of spatial multiplexing streams when the number of available precoding matrices, i.e., the codebook size, is less than approximately 16 and 32 for 2-by-2 and 4-by-4 MIMO multiplexing, respectively.

Selective impairment of hippocampus and posterior hub areas in Alzheimer's disease: an MEG-based multiplex network study.

PubMed

Yu, Meichen; Engels, Marjolein M A; Hillebrand, Arjan; van Straaten, Elisabeth C W; Gouw, Alida A; Teunissen, Charlotte; van der Flier, Wiesje M; Scheltens, Philip; Stam, Cornelis J

2017-05-01

Although frequency-specific network analyses have shown that functional brain networks are altered in patients with Alzheimer's disease, the relationships between these frequency-specific network alterations remain largely unknown. Multiplex network analysis is a novel network approach to study complex systems consisting of subsystems with different types of connectivity patterns. In this study, we used magnetoencephalography to integrate five frequency-band specific brain networks in a multiplex framework. Previous structural and functional brain network studies have consistently shown that hub brain areas are selectively disrupted in Alzheimer's disease. Accordingly, we hypothesized that hub regions in the multiplex brain networks are selectively targeted in patients with Alzheimer's disease in comparison to healthy control subjects. Eyes-closed resting-state magnetoencephalography recordings from 27 patients with Alzheimer's disease (60.6 ± 5.4 years, 12 females) and 26 controls (61.8 ± 5.5 years, 14 females) were projected onto atlas-based regions of interest using beamforming. Subsequently, source-space time series for both 78 cortical and 12 subcortical regions were reconstructed in five frequency bands (delta, theta, alpha 1, alpha 2 and beta band). Multiplex brain networks were constructed by integrating frequency-specific magnetoencephalography networks. Functional connections between all pairs of regions of interests were quantified using a phase-based coupling metric, the phase lag index. Several multiplex hub and heterogeneity metrics were computed to capture both overall importance of each brain area and heterogeneity of the connectivity patterns across frequency-specific layers. Different nodal centrality metrics showed consistently that several hub regions, particularly left hippocampus, posterior parts of the default mode network and occipital regions, were vulnerable in patients with Alzheimer's disease compared to control subjects. Of note, these detected vulnerable hubs in Alzheimer's disease were absent in each individual frequency-specific network, thus showing the value of integrating the networks. The connectivity patterns of these vulnerable hub regions in the patients were heterogeneously distributed across layers. Perturbed cognitive function and abnormal cerebrospinal fluid amyloid-β42 levels correlated positively with the vulnerability of the hub regions in patients with Alzheimer's disease. Our analysis therefore demonstrates that the magnetoencephalography-based multiplex brain networks contain important information that cannot be revealed by frequency-specific brain networks. Furthermore, this indicates that functional networks obtained in different frequency bands do not act as independent entities. Overall, our multiplex network study provides an effective framework to integrate the frequency-specific networks with different frequency patterns and reveal neuropathological mechanism of hub disruption in Alzheimer's disease. © The Author (2017). Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Telemetry Standards, RCC Standard 106-17. Chapter 3. Frequency Division Multiplexing Telemetry Standards

DTIC Science & Technology

2017-07-01

any of the listed reference frequencies may be used provided the requirements for compensation rate of change are satisfied. If the reference...for in present discriminator systems when the nominal response rating of the channels is employed and a reference frequency is recorded with the...Telemetry Standards, RCC Standard 106-17 Chapter 3, July 2017 3-i CHAPTER 3 Frequency Division Multiplexing Telemetry Standards Acronyms

Precision control of multiple quantum cascade lasers for calibration systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Taubman, Matthew S., E-mail: Matthew.Taubman@pnnl.gov; Myers, Tanya L.; Pratt, Richard M.

We present a precision, 1-A, digitally interfaced current controller for quantum cascade lasers, with demonstrated temperature coefficients for continuous and 40-kHz full-depth square-wave modulated operation, of 1–2 ppm/ °C and 15 ppm/ °C, respectively. High precision digital to analog converters (DACs) together with an ultra-precision voltage reference produce highly stable, precision voltages, which are selected by a multiplexer (MUX) chip to set output currents via a linear current regulator. The controller is operated in conjunction with a power multiplexing unit, allowing one of three lasers to be driven by the controller, while ensuring protection of controller and all lasers during operation, standby,more » and switching. Simple ASCII commands sent over a USB connection to a microprocessor located in the current controller operate both the controller (via the DACs and MUX chip) and the power multiplexer.« less

Precision Control of Multiple Quantum Cascade Lasers for Calibration Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Taubman, Matthew S.; Myers, Tanya L.; Pratt, Richard M.

We present a precision, digitally interfaced current controller for quantum cascade lasers, with demonstrated DC and modulated temperature coefficients of 1- 2 ppm/ºC and 15 ppm/ºC respectively. High linearity digital to analog converters (DACs) together with an ultra-precision voltage reference, produce highly stable, precision voltages. These are in turn selected by a low charge-injection multiplexer (MUX) chip, which are then used to set output currents via a linear current regulator. The controller is operated in conjunction with a power multiplexing unit, allowing one of three lasers to be driven by the controller while ensuring protection of controller and all lasersmore » during operation, standby and switching. Simple ASCII commands sent over a USB connection to a microprocessor located in the current controller operate both the controller (via the DACs and MUX chip) and the power multiplexer.« less

Novel Quaternary Quantum Decoder, Multiplexer and Demultiplexer Circuits

NASA Astrophysics Data System (ADS)

Haghparast, Majid; Monfared, Asma Taheri

2017-05-01

Multiple valued logic is a promising approach to reduce the width of the reversible or quantum circuits, moreover, quaternary logic is considered as being a good choice for future quantum computing technology hence it is very suitable for the encoded realization of binary logic functions through its grouping of 2-bits together into quaternary values. The Quaternary decoder, multiplexer, and demultiplexer are essential units of quaternary digital systems. In this paper, we have initially designed a quantum realization of the quaternary decoder circuit using quaternary 1-qudit gates and quaternary Muthukrishnan-Stroud gates. Then we have presented quantum realization of quaternary multiplexer and demultiplexer circuits using the constructed quaternary decoder circuit and quaternary controlled Feynman gates. The suggested circuits in this paper have a lower quantum cost and hardware complexity than the existing designs that are currently used in quaternary digital systems. All the scales applied in this paper are based on Nanometric area.

Spatial Frequency Multiplexing of Fiber-Optic Interferometric Refractive Index Sensors Based on Graded-Index Multimode Fibers

PubMed Central

Liu, Li; Gong, Yuan; Wu, Yu; Zhao, Tian; Wu, Hui-Juan; Rao, Yun-Jiang

2012-01-01

Fiber-optic interferometric sensors based on graded-index multimode fibers have very high refractive-index sensitivity, as we previously demonstrated. In this paper, spatial-frequency multiplexing of this type of fiber-optic refractive index sensors is investigated. It is estimated that multiplexing of more than 10 such sensors is possible. In the multiplexing scheme, one of the sensors is used to investigate the refractive index and temperature responses. The fast Fourier transform (FFT) of the combined reflective spectra is analyzed. The intensity of the FFT spectra is linearly related with the refractive index and is not sensitive to the temperature.

Multiplex Ultrasensitive Genotyping of Patients with Non-Small Cell Lung Cancer for Epidermal Growth Factor Receptor (EGFR) Mutations by Means of Picodroplet Digital PCR.

PubMed

Watanabe, Masaru; Kawaguchi, Tomoya; Isa, Shun-Ichi; Ando, Masahiko; Tamiya, Akihiro; Kubo, Akihito; Saka, Hideo; Takeo, Sadanori; Adachi, Hirofumi; Tagawa, Tsutomu; Kawashima, Osamu; Yamashita, Motohiro; Kataoka, Kazuhiko; Ichinose, Yukito; Takeuchi, Yukiyasu; Watanabe, Katsuya; Matsumura, Akihide; Koh, Yasuhiro

2017-07-01

Epidermal growth factor receptor (EGFR) mutations have been used as the strongest predictor of effectiveness of treatment with EGFR tyrosine kinase inhibitors (TKIs). Three most common EGFR mutations (L858R, exon 19 deletion, and T790M) are known to be major selection markers for EGFR-TKIs therapy. Here, we developed a multiplex picodroplet digital PCR (ddPCR) assay to detect 3 common EGFR mutations in 1 reaction. Serial-dilution experiments with genomic DNA harboring EGFR mutations revealed linear performance, with analytical sensitivity ~0.01% for each mutation. All 33 EGFR-activating mutations detected in formalin-fixed paraffin-embedded (FFPE) tissue samples by the conventional method were also detected by this multiplex assay. Owing to the higher sensitivity, an additional mutation (T790M; including an ultra-low-level mutation, <0.1%) was detected in the same reaction. Regression analysis of the duplex assay and multiplex assay showed a correlation coefficient (R 2 ) of 0.9986 for L858R, 0.9844 for an exon 19 deletion, and 0.9959 for T790M. Using ddPCR, we designed a multiplex ultrasensitive genotyping platform for 3 common EGFR mutations. Results of this proof-of-principle study on clinical samples indicate clinical utility of multiplex ddPCR for screening for multiple EGFR mutations concurrently with an ultra-rare pretreatment mutation (T790M). Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Mapping Multiplex Hubs in Human Functional Brain Networks

PubMed Central

De Domenico, Manlio; Sasai, Shuntaro; Arenas, Alex

2016-01-01

Typical brain networks consist of many peripheral regions and a few highly central ones, i.e., hubs, playing key functional roles in cerebral inter-regional interactions. Studies have shown that networks, obtained from the analysis of specific frequency components of brain activity, present peculiar architectures with unique profiles of region centrality. However, the identification of hubs in networks built from different frequency bands simultaneously is still a challenging problem, remaining largely unexplored. Here we identify each frequency component with one layer of a multiplex network and face this challenge by exploiting the recent advances in the analysis of multiplex topologies. First, we show that each frequency band carries unique topological information, fundamental to accurately model brain functional networks. We then demonstrate that hubs in the multiplex network, in general different from those ones obtained after discarding or aggregating the measured signals as usual, provide a more accurate map of brain's most important functional regions, allowing to distinguish between healthy and schizophrenic populations better than conventional network approaches. PMID:27471443

CFO compensation method using optical feedback path for coherent optical OFDM system

NASA Astrophysics Data System (ADS)

Moon, Sang-Rok; Hwang, In-Ki; Kang, Hun-Sik; Chang, Sun Hyok; Lee, Seung-Woo; Lee, Joon Ki

2017-07-01

We investigate feasibility of carrier frequency offset (CFO) compensation method using optical feedback path for coherent optical orthogonal frequency division multiplexing (CO-OFDM) system. Recently proposed CFO compensation algorithms provide wide CFO estimation range in electrical domain. However, their practical compensation range is limited by sampling rate of an analog-to-digital converter (ADC). This limitation has not drawn attention, since the ADC sampling rate was high enough comparing to the data bandwidth and CFO in the wireless OFDM system. For CO-OFDM, the limitation is becoming visible because of increased data bandwidth, laser instability (i.e. large CFO) and insufficient ADC sampling rate owing to high cost. To solve the problem and extend practical CFO compensation range, we propose a CFO compensation method having optical feedback path. By adding simple wavelength control for local oscillator, the practical CFO compensation range can be extended to the sampling frequency range. The feasibility of the proposed method is experimentally investigated.

Multiplexed enrichment of rare DNA variants via sequence-selective and temperature-robust amplification

PubMed Central

Wu, Lucia R.; Chen, Sherry X.; Wu, Yalei; Patel, Abhijit A.; Zhang, David Yu

2018-01-01

Rare DNA-sequence variants hold important clinical and biological information, but existing detection techniques are expensive, complex, allele-specific, or don’t allow for significant multiplexing. Here, we report a temperature-robust polymerase-chain-reaction method, which we term blocker displacement amplification (BDA), that selectively amplifies all sequence variants, including single-nucleotide variants (SNVs), within a roughly 20-nucleotide window by 1,000-fold over wild-type sequences. This allows for easy detection and quantitation of hundreds of potential variants originally at ≤0.1% in allele frequency. BDA is compatible with inexpensive thermocycler instrumentation and employs a rationally designed competitive hybridization reaction to achieve comparable enrichment performance across annealing temperatures ranging from 56 °C to 64 °C. To show the sequence generality of BDA, we demonstrate enrichment of 156 SNVs and the reliable detection of single-digit copies. We also show that the BDA detection of rare driver mutations in cell-free DNA samples extracted from the blood plasma of lung-cancer patients is highly consistent with deep sequencing using molecular lineage tags, with a receiver operator characteristic accuracy of 95%. PMID:29805844

Space and frequency-multiplexed optical linear algebra processor - Fabrication and initial tests

NASA Technical Reports Server (NTRS)

Casasent, D.; Jackson, J.

1986-01-01

A new optical linear algebra processor architecture is described. Space and frequency-multiplexing are used to accommodate bipolar and complex-valued data. A fabricated laboratory version of this processor is described, the electronic support system used is discussed, and initial test data obtained on it are presented.

High-volume optical vortex multiplexing and de-multiplexing for free-space optical communication.

PubMed

Wang, Zhongxi; Zhang, N; Yuan, X-C

2011-01-17

We report an approach to the increase of signal channels in free-space optical communication based on composed optical vortices (OVs). In the encoding process, conventional algorithm employed for the generation of collinearly superimposed OVs is combined with a genetic algorithm to achieve high-volume OV multiplexing. At the receiver end, a novel Dammann vortex grating is used to analyze the multihelix beams with a large number of OVs. We experimentally demonstrate a digitized system which is capable of transmitting and receiving 16 OV channels simultaneously. This system is expected to be compatible with a high-speed OV multiplexing technique, with potentials to extremely high-volume information density in OV communication.

A hybrid single-end-access MZI and Φ-OTDR vibration sensing system with high frequency response

NASA Astrophysics Data System (ADS)

Zhang, Yixin; Xia, Lan; Cao, Chunqi; Sun, Zhenhong; Li, Yanting; Zhang, Xuping

2017-01-01

A hybrid single-end-access Mach-Zehnder interferometer (MZI) and phase sensitive OTDR (Φ-OTDR) vibration sensing system is proposed and demonstrated experimentally. In our system, the narrow optical pulses and the continuous wave are injected into the fiber through the front end of the fiber at the same time. And at the rear end of the fiber, a frequency-shift-mirror (FSM) is designed to back propagate the continuous wave modulated by the external vibration. Thus the Rayleigh backscattering signals (RBS) and the back propagated continuous wave interfere with the reference light at the same end of the sensing fiber and a single-end-access configuration is achieved. The RBS can be successfully separated from the interference signal (IS) through digital signal process due to their different intermediate frequency based on frequency division multiplexing technique. There is no influence between these two schemes. The experimental results show 10 m spatial resolution and up to 1.2 MHz frequency response along a 6.35 km long fiber. This newly designed single-end-access setup can achieve vibration events locating and high frequency events response, which can be widely used in health monitoring for civil infrastructures and transportation.

Holographic memory system based on projection recording of computer-generated 1D Fourier holograms.

PubMed

Betin, A Yu; Bobrinev, V I; Donchenko, S S; Odinokov, S B; Evtikhiev, N N; Starikov, R S; Starikov, S N; Zlokazov, E Yu

2014-10-01

Utilization of computer generation of holographic structures significantly simplifies the optical scheme that is used to record the microholograms in a holographic memory record system. Also digital holographic synthesis allows to account the nonlinear errors of the record system to improve the microholograms quality. The multiplexed record of holograms is a widespread technique to increase the data record density. In this article we represent the holographic memory system based on digital synthesis of amplitude one-dimensional (1D) Fourier transform holograms and the multiplexed record of these holograms onto the holographic carrier using optical projection scheme. 1D Fourier transform holograms are very sensitive to orientation of the anamorphic optical element (cylindrical lens) that is required for encoded data object reconstruction. The multiplex record of several holograms with different orientation in an optical projection scheme allowed reconstruction of the data object from each hologram by rotating the cylindrical lens on the corresponding angle. Also, we discuss two optical schemes for the recorded holograms readout: a full-page readout system and line-by-line readout system. We consider the benefits of both systems and present the results of experimental modeling of 1D Fourier holograms nonmultiplex and multiplex record and reconstruction.

Multifrequency OFDM SAR in Presence of Deception Jamming

NASA Astrophysics Data System (ADS)

Schuerger, Jonathan; Garmatyuk, Dmitriy

2010-12-01

Orthogonal frequency division multiplexing (OFDM) is considered in this paper from the perspective of usage in imaging radar scenarios with deception jamming. OFDM radar signals are inherently multifrequency waveforms, composed of a number of subbands which are orthogonal to each other. While being employed extensively in communications, OFDM has not found comparatively wide use in radar, and, particularly, in synthetic aperture radar (SAR) applications. In this paper, we aim to show the advantages of OFDM-coded radar signals with random subband composition when used in deception jamming scenarios. Two approaches to create a radar signal by the jammer are considered: instantaneous frequency (IF) estimator and digital-RF-memory- (DRFM-) based reproducer. In both cases, the jammer aims to create a copy of a valid target image via resending the radar signal at prescribed time intervals. Jammer signals are derived and used in SAR simulations with three types of signal models: OFDM, linear frequency modulated (LFM), and frequency-hopped (FH). Presented results include simulated peak side lobe (PSL) and peak cross-correlation values for random OFDM signals, as well as simulated SAR imagery with IF and DRFM jammers'-induced false targets.

Applications of surface acoustic and shallow bulk acoustic wave devices

NASA Astrophysics Data System (ADS)

Campbell, Colin K.

1989-10-01

Surface acoustic wave (SAW) device coverage includes delay lines and filters operating at selected frequencies in the range from about 10 MHz to 11 GHz; modeling with single-crystal piezoelectrics and layered structures; resonators and low-loss filters; comb filters and multiplexers; antenna duplexers; harmonic devices; chirp filters for pulse compression; coding with fixed and programmable transversal filters; Barker and quadraphase coding; adaptive filters; acoustic and acoustoelectric convolvers and correlators for radar, spread spectrum, and packet radio; acoustooptic processors for Bragg modulation and spectrum analysis; real-time Fourier-transform and cepstrum processors for radar and sonar; compressive receivers; Nyquist filters for microwave digital radio; clock-recovery filters for fiber communications; fixed-, tunable-, and multimode oscillators and frequency synthesizers; acoustic charge transport; and other SAW devices for signal processing on gallium arsenide. Shallow bulk acoustic wave device applications include gigahertz delay lines, surface-transverse-wave resonators employing energy-trapping gratings, and oscillators with enhanced performance and capability.

Dual-drive Mach-Zehnder modulator-based reconfigurable and transparent spectral conversion for dense wavelength division multiplexing transmissions

NASA Astrophysics Data System (ADS)

Mao, Mingzhi; Qian, Chen; Cao, Bingyao; Zhang, Qianwu; Song, Yingxiong; Wang, Min

2017-09-01

A digital signal process enabled dual-drive Mach-Zehnder modulator (DD-MZM)-based spectral converter is proposed and extensively investigated to realize dynamically reconfigurable and high transparent spectral conversion. As another important innovation point of the paper, to optimize the converter performance, the optimum operation conditions of the proposed converter are deduced, statistically simulated, and experimentally verified. The optimum conditions supported-converter performances are verified by detail numerical simulations and experiments in intensity-modulation and direct-detection-based network in terms of frequency detuning range-dependent conversion efficiency, strict operation transparency for user signal characteristics, impact of parasitic components on the conversion performance, as well as the converted component waveform are almost nondistortion. It is also found that the converter has the high robustness to the input signal power, optical signal-to-noise ratio variations, extinction ratio, and driving signal frequency.

Wired/wireless access integrated RoF-PON with scalable generation of multi-frequency MMWs enabled by polarization multiplexed FWM in SOA.

PubMed

Xiang, Yu; Chen, Chen; Zhang, Chongfu; Qiu, Kun

2013-01-14

In this paper, we propose and demonstrate a novel integrated radio-over-fiber passive optical network (RoF-PON) system for both wired and wireless access. By utilizing the polarization multiplexed four-wave mixing (FWM) effect in a semiconductor optical amplifier (SOA), scalable generation of multi-frequency millimeter-waves (MMWs) can be provided so as to assist the configuration of multi-frequency wireless access for the wire/wireless access integrated ROF-PON system. In order to obtain a better performance, the polarization multiplexed FWM effect is investigated in detail. Simulation results successfully verify the feasibility of our proposed scheme.

Quality Control of Next-generation Sequencing-based In vitro Diagnostic Test for Onco-relevant Mutations Using Multiplex Reference Materials in Plasma.

PubMed

Liu, Donglai; Zhou, Haiwei; Shi, Dawei; Shen, Shu; Tian, Yabin; Wang, Lin; Lou, Jiatao; Cong, Rong; Lu, Juan; Zhang, Henghui; Zhao, Meiru; Zhu, Shida; Cao, Zhisheng; Jin, Ruilin; Wang, Yin; Zhang, Xiaoni; Yang, Guohua; Wang, Youchun; Zhang, Chuntao

2018-01-01

Background: Widespread clinical implementation of next-generation sequencing (NGS)-based cancer in vitro diagnostic tests (IVDs) highlighted the urgency to establish reference materials which could provide full control of the process from nucleic acid extraction to test report generation. The formalin-fixed, paraffin-embedded (FFPE) tissue and blood plasma containing circulating tumor deoxyribonucleic acid (ctDNA) were mostly used for clinically detecting onco-relevant mutations. Methods: We respectively developed multiplex FFPE and plasma reference materials covering three clinically onco-relevant mutations within the epidermal growth factor receptor ( EGFR ) gene at serial allelic frequencies. All reference materials were quantified and validated via droplet digital polymerase chain reaction (ddPCR), and then were distributed to eight domestic manufacturers for the collaborative evaluation of the performance of several domestic NGS-based cancer IVDs covering four major NGS platforms (NextSeq, HiSeq, Ion Proton and BGISEQ). Results: All expected mutations except one at extremely low allelic frequencies were detected, despite some differences in coefficient of variation (CV) which increased with the decrease of allelic frequency (CVs ranging from 18% to 106%). It was worth noting that the CV value seemed to correlate with a particular mutation as well. The repeatability of determination of different mutations was L858R>T790M>19del. Conclusions: The results indicated our reference materials would be pivotal for quality control of NGS-based cancer IVDs and would guide the further development of reference materials covering more onco-relevant mutations.

Convergent optical wired and wireless long-reach access network using high spectral-efficient modulation.

PubMed

Chow, C W; Lin, Y H

2012-04-09

To provide broadband services in a single and low cost perform, the convergent optical wired and wireless access network is promising. Here, we propose and demonstrate a convergent optical wired and wireless long-reach access networks based on orthogonal wavelength division multiplexing (WDM). Both the baseband signal and the radio-over-fiber (ROF) signal are multiplexed and de-multiplexed in optical domain, hence it is simple and the operation speed is not limited by the electronic bottleneck caused by the digital signal processing (DSP). Error-free de-multiplexing and down-conversion can be achieved for all the signals after 60 km (long-reach) fiber transmission. The scalability of the system for higher bit-rate (60 GHz) is also simulated and discussed.

Underwater optical communications using orbital angular momentum-based spatial division multiplexing

NASA Astrophysics Data System (ADS)

Willner, Alan E.; Zhao, Zhe; Ren, Yongxiong; Li, Long; Xie, Guodong; Song, Haoqian; Liu, Cong; Zhang, Runzhou; Bao, Changjing; Pang, Kai

2018-02-01

In this paper, we review high-capacity underwater optical communications using orbital angular momentum (OAM)-based spatial division multiplexing. We discuss methods to generate and detect blue-green optical data-carrying OAM beams as well as various underwater effects, including attenuation, scattering, current, and thermal gradients on OAM beams. Attention is also given to the system performance of high-capacity underwater optical communication links using OAM-based space division multiplexing. The paper closes with a discussion of a digital signal processing (DSP) algorithm to mitigate the inter-mode crosstalk caused by thermal gradients.

Cross talk and diffraction efficiency in angular multiplexed memories using improved polypeptide

NASA Astrophysics Data System (ADS)

Ramenah, Harry K.; Bertrand, Paul; Soubari, E. H.; Meyrueis, Patrick

1996-12-01

We studied energy coupling between gratings and angularly multiplexed 20 gratings with a uniform diffraction efficiency within 25 micrometer layer thickness of dichromated gelatin. The dependence of diffraction efficiency on beam ratio is given. We recorded a matrix form memory of nxmxp elements, where n and m are the rows and columns and p the number of multiplexes. For indication only, n equals m equals 10, p equals 20, the surface area of the matrix is 1 cm2. Color diffractive images and digital data are illustrated as well as video, cartography and medical applications.

Effects of HPA-nonlinearity on a 4-DPSK/OFDM-signal for a digital sound broadcasting signal

NASA Astrophysics Data System (ADS)

Rapp, Christoph

1991-10-01

Orthogonal Frequency Division Multiplexing (OFDM) in conjunction with a 4 Differential Phase Shift Keying (DPSK) modulation format has been proposed for the future Digital Audio Broadcasting system (DAB), that should provide compact disk sound quality in portable, vehicular and fixed receivers. With properly chosen parameters, this system should be appropriate for both terrestrial and satellite transmission. The influence of the nonlinear distorsions introduced by the High Power Amplifier (HPA) of the transmitter is examined. In particular, the degradations in power efficiency due to intermodulation effects and backoff operating, as well as spectral degradations are investigated. It is shown for three different kinds of limiting amplifier models, that even with an output backoff in the region of 5 to 6 dB, the degradation of, for example a 512 carrier 4 DPSK/OFDM system relative to the linear case is below 1.7 dB (Pb = 0.0001), while the regenerated sidelobes of the transmitted spectrum are kept below -20 dB.

Fast convergent frequency-domain MIMO equalizer for few-mode fiber communication systems

NASA Astrophysics Data System (ADS)

He, Xuan; Weng, Yi; Wang, Junyi; Pan, Z.

2018-02-01

Space division multiplexing using few-mode fibers has been extensively explored to sustain the continuous traffic growth. In few-mode fiber optical systems, both spatial and polarization modes are exploited to transmit parallel channels, thus increasing the overall capacity. However, signals on spatial channels inevitably suffer from the intrinsic inter-modal coupling and large accumulated differential mode group delay (DMGD), which causes spatial modes de-multiplex even harder. Many research articles have demonstrated that frequency domain adaptive multi-input multi-output (MIMO) equalizer can effectively compensate the DMGD and demultiplex the spatial channels with digital signal processing (DSP). However, the large accumulated DMGD usually requires a large number of training blocks for the initial convergence of adaptive MIMO equalizers, which will decrease the overall system efficiency and even degrade the equalizer performance in fast-changing optical channels. Least mean square (LMS) algorithm is always used in MIMO equalization to dynamically demultiplex the spatial signals. We have proposed to use signal power spectral density (PSD) dependent method and noise PSD directed method to improve the convergence speed of adaptive frequency domain LMS algorithm. We also proposed frequency domain recursive least square (RLS) algorithm to further increase the convergence speed of MIMO equalizer at cost of greater hardware complexity. In this paper, we will compare the hardware complexity and convergence speed of signal PSD dependent and noise power directed algorithms against the conventional frequency domain LMS algorithm. In our numerical study of a three-mode 112 Gbit/s PDM-QPSK optical system with 3000 km transmission, the noise PSD directed and signal PSD dependent methods could improve the convergence speed by 48.3% and 36.1% respectively, at cost of 17.2% and 10.7% higher hardware complexity. We will also compare the frequency domain RLS algorithm against conventional frequency domain LMS algorithm. Our numerical study shows that, in a three-mode 224 Gbit/s PDM-16-QAM system with 3000 km transmission, the RLS algorithm could improve the convergence speed by 53.7% over conventional frequency domain LMS algorithm.

The DCU: the detector control unit of the SAFARI instrument onboard SPICA

NASA Astrophysics Data System (ADS)

Clénet, A.; Ravera, L.; Bertrand, B.; Cros, A.; Hou, R.; Jackson, B. D.; van Leeuwen, B. J.; Van Loon, D.; Parot, Y.; Pointecouteau, E.; Sournac, A.; Ta, N.

2012-09-01

The SpicA FAR infrared Instrument (SAFARI) is a European instrument for the infrared domain telescope SPICA, a JAXA space mission. The SAFARI detectors are Transistor Edge Sensors (TES) arranged in 3 matrixes. The TES front end electronic is based on Superconducting Quantum Interference Devices (SQUIDs) and it does the readout of the 3500 detectors with Frequency Division Multiplexing (FDM) type architecture. The Detector Control Unit (DCU), contributed by IRAP, manages the readout of the TES by computing and providing the AC-bias signals (1 - 3 MHz) to the TES and by computing the demodulation of the returning signals. The SQUID being highly non-linear, the DCU has also to provide a feedback signal to increase the SQUID dynamic. Because of the propagation delay in the cables and the processing time, a classic feedback will not be stable for AC-bias frequencies up to 3 MHz. The DCU uses a specific technique to compensate for those delays: the BaseBand FeedBack (BBFB). This digital data processing is done for the 3500 pixels in parallel. Thus, to keep the DCU power budget within its allocation we have to specifically optimize the architecture of the digital circuit with respect to the power consumption. In this paper we will mainly present the DCU architecture. We will particularly focus on the BBFB technique used to linearize the SQUID and on the optimization done to reduce the power consumption of the digital processing circuit.

On-Board Fiber-Optic Network Architectures for Radar and Avionics Signal Distribution

NASA Technical Reports Server (NTRS)

Alam, Mohammad F.; Atiquzzaman, Mohammed; Duncan, Bradley B.; Nguyen, Hung; Kunath, Richard

2000-01-01

Continued progress in both civil and military avionics applications is overstressing the capabilities of existing radio-frequency (RF) communication networks based on coaxial cables on board modem aircrafts. Future avionics systems will require high-bandwidth on- board communication links that are lightweight, immune to electromagnetic interference, and highly reliable. Fiber optic communication technology can meet all these challenges in a cost-effective manner. Recently, digital fiber-optic communication systems, where a fiber-optic network acts like a local area network (LAN) for digital data communications, have become a topic of extensive research and development. Although a fiber-optic system can be designed to transport radio-frequency (RF) signals, the digital fiber-optic systems under development today are not capable of transporting microwave and millimeter-wave RF signals used in radar and avionics systems on board an aircraft. Recent advances in fiber optic technology, especially wavelength division multiplexing (WDM), has opened a number of possibilities for designing on-board fiber optic networks, including all-optical networks for radar and avionics RF signal distribution. In this paper, we investigate a number of different novel approaches for fiber-optic transmission of on-board VHF and UHF RF signals using commercial off-the-shelf (COTS) components. The relative merits and demerits of each architecture are discussed, and the suitability of each architecture for particular applications is pointed out. All-optical approaches show better performance than other traditional approaches in terms of signal-to-noise ratio, power consumption, and weight requirements.

Rapid updating of optical arbitrary waveforms via time-domain multiplexing.

PubMed

Scott, R P; Fontaine, N K; Yang, C; Geisler, D J; Okamoto, K; Heritage, J P; Yoo, S J B

2008-05-15

We demonstrate high-fidelity optical arbitrary waveform generation with 5 GHz waveform switching via time-domain multiplexing. Compact, integrated waveform shapers based on silica arrayed-waveguide grating pairs with 10 GHz channel spacing are used to shape (line-by-line) two different waveforms from the output of a 10-mode x 10 GHz optical frequency comb generator. Characterization of the time multiplexer's complex transfer function (amplitude and phase) by frequency-resolved optical gating permits compensation of its impact on the switched waveforms and matching of the measured and target waveforms to better than G'=5%.

Investigation into constant envelope orthogonal frequency division multiplexing for polarization-division multiplexing coherent optical communication

NASA Astrophysics Data System (ADS)

Li, Yupeng; Ding, Ding

2017-09-01

Benefiting from the high spectral efficiency and low peak-to-average power ratio, constant envelope orthogonal frequency division multiplexing (OFDM) is a promising technique in coherent optical communication. Polarization-division multiplexing (PDM) has been employed as an effective way to double the transmission capacity in the commercial 100 Gb/s PDM-QPSK system. We investigated constant envelope OFDM together with PDM. Simulation results show that the acceptable maximum launch power into the fiber improves 10 and 6 dB for 80- and 320-km transmission, respectively (compared with the conventional PDM OFDM system). The maximum reachable distance of the constant envelope OFDM system is able to reach 800 km, and even 1200 km is reachable if an ideal erbium doped fiber amplifier is employed.

Multiplex picodroplet digital PCR to detect KRAS mutations in circulating DNA from the plasma of colorectal cancer patients.

PubMed

Taly, Valerie; Pekin, Deniz; Benhaim, Leonor; Kotsopoulos, Steve K; Le Corre, Delphine; Li, Xinyu; Atochin, Ivan; Link, Darren R; Griffiths, Andrew D; Pallier, Karine; Blons, Hélène; Bouché, Olivier; Landi, Bruno; Hutchison, J Brian; Laurent-Puig, Pierre

2013-12-01

Multiplex digital PCR (dPCR) enables noninvasive and sensitive detection of circulating tumor DNA with performance unachievable by current molecular-detection approaches. Furthermore, picodroplet dPCR facilitates simultaneous screening for multiple mutations from the same sample. We investigated the utility of multiplex dPCR to screen for the 7 most common mutations in codons 12 and 13 of the KRAS (Kirsten rat sarcoma viral oncogene homolog) oncogene from plasma samples of patients with metastatic colorectal cancer. Fifty plasma samples were tested from patients for whom the primary tumor biopsy tissue DNA had been characterized by quantitative PCR. Tumor characterization revealed that 19 patient tumors had KRAS mutations. Multiplex dPCR analysis of the plasma DNA prepared from these samples identified 14 samples that matched the mutation identified in the tumor, 1 sample contained a different KRAS mutation, and 4 samples had no detectable mutation. Among the tumor samples that were wild type for KRAS, 2 KRAS mutations were identified in the corresponding plasma samples. Duplex dPCR (i.e., wild-type and single-mutation assay) was also used to analyze plasma samples from patients with KRAS-mutated tumors and 5 samples expected to contain the BRAF (v-raf murine sarcoma viral oncogene homolog B) V600E mutation. The results for the duplex analysis matched those for the multiplex analysis for KRAS-mutated samples and, owing to its higher sensitivity, enabled detection of 2 additional samples with low levels of KRAS-mutated DNA. All 5 samples with BRAF mutations were detected. This work demonstrates the clinical utility of multiplex dPCR to screen for multiple mutations simultaneously with a sensitivity sufficient to detect mutations in circulating DNA obtained by noninvasive blood collection.

Novel application of digital microfluidics for the detection of biotinidase deficiency in newborns.

PubMed

Graham, Carrie; Sista, Ramakrishna S; Kleinert, Jairus; Wu, Ning; Eckhardt, Allen; Bali, Deeksha; Millington, David S; Pamula, Vamsee K

2013-12-01

Newborn screening for biotinidase deficiency can be performed using a fluorometric enzyme assay on dried blood spot specimens. As a pre-requisite to the consolidation of different enzymatic assays onto a single platform, we describe here a novel analytical method for detecting biotinidase deficiency using the same digital microfluidic cartridge that has already been demonstrated to screen for five lysosomal storage diseases (Pompe, Fabry, Gaucher, Hurler and Hunter) in a multiplex format. A novel assay to quantify biotinidase concentration in dried blood spots (DBS) was developed and optimized on the digital microfluidic platform using proficiency testing samples from the Centers for Disease Control and Prevention. The enzymatic assay uses 4-methylumbelliferyl biotin as the fluorogenic substrate. Biotinidase deficiency assays were performed on normal (n=200) and deficient (n=7) newborn DBS specimens. Enzymatic activity analysis of biotinidase deficiency revealed distinct separation between normal and affected DBS specimens using digital microfluidics and these results matched the expected activity. This study has demonstrated performance of biotinidase deficiency assays by measurement of 4-methylumbelliferyl product on a digital microfluidic platform. Due to the inherent ease in multiplexing on such a platform, consolidation of other fluorometric assays onto a single cartridge may be realized. © 2013.

Experiment on Synchronous Timing Signal Detection from ISDB-T Terrestrial Digital TV Signal with Application to Autonomous Distributed ITS-IVC Network

NASA Astrophysics Data System (ADS)

Karasawa, Yoshio; Kumagai, Taichi; Takemoto, Atsushi; Fujii, Takeo; Ito, Kenji; Suzuki, Noriyoshi

A novel timing synchronizing scheme is proposed for use in inter-vehicle communication (IVC) with an autonomous distributed intelligent transport system (ITS). The scheme determines the timing of packet signal transmission in the IVC network and employs the guard interval (GI) timing in the orthogonal frequency divisional multiplexing (OFDM) signal currently used for terrestrial broadcasts in the Japanese digital television system (ISDB-T). This signal is used because it is expected that the automotive market will demand the capability for cars to receive terrestrial digital TV broadcasts in the near future. The use of broadcasts by automobiles presupposes that the on-board receivers are capable of accurately detecting the GI timing data in an extremely low carrier-to-noise ratio (CNR) condition regardless of a severe multipath environment which will introduce broad scatter in signal arrival times. Therefore, we analyzed actual broadcast signals received in a moving vehicle in a field experiment and showed that the GI timing signal is detected with the desired accuracy even in the case of extremely low-CNR environments. Some considerations were also given about how to use these findings.

Screening for circulating RAS/RAF mutations by multiplex digital PCR.

PubMed

Andersen, Rikke Fredslund; Jakobsen, Anders

2016-07-01

Recent years have shown a large interest in the application of liquid biopsies in cancer management. Circulating tumor DNA (ctDNA) has been investigated for potential use in treatment selection, monitoring of treatment response, and early detection of recurrence. Advances have been hampered by technical challenges primarily due to the low levels of ctDNA in patients with localized disease and in patients responding to therapy. The approach presented here is a multiplex digital PCR method of screening for 31 mutations in the KRAS, NRAS, BRAF, and PIK3CA genes in the plasma. The upper level of the limit of blank, which defines the specificity of the multiplexes, was 0.006%-0.06%. Mutations found by multiplex analyses were identified and quantified by duplex analyses. The method was tested on samples from cholangiocarcinoma patients with known tumor mutational status. Mutations found in the tumor were also found in plasma samples in all cases with analyses for all other mutations being negative. There was a perfect agreement as to wild type status in tumor and plasma. The method combines a high sensitivity with the ability to analyze for several mutations at a time and could be a step towards routine clinical application of liquid biopsies. Copyright © 2016 Elsevier B.V. All rights reserved.

Light-Addressed Electrodeposition of Enzyme-Entrapped Chitosan Membranes for Multiplexed Enzyme-Based Bioassays Using a Digital Micromirror Device

PubMed Central

Huang, Shih-Hao; Wei, Lu-Shiuan; Chu, Hsiao-Tzu; Jiang, Yeu-Long

2013-01-01

This paper describes a light-addressed electrolytic system used to perform an electrodeposition of enzyme-entrapped chitosan membranes for multiplexed enzyme-based bioassays using a digital micromirror device (DMD). In this system, a patterned light illumination is projected onto a photoconductive substrate serving as a photo-cathode to electrolytically produce hydroxide ions, which leads to an increased pH gradient. The high pH generated at the cathode can cause a local gelation of chitosan through sol-gel transition. By controlling the illumination pattern on the DMD, a light-addressed electrodeposition of chitosan membranes with different shapes and sizes, as well as multiplexed micropatterning, was performed. The effect of the illumination time of the light pattern on the dimensional resolution of chitosan membrane formation was examined experimentally. Moreover, multiplexed enzyme-based bioassay of enzyme-entrapped chitosan membranes was also successfully demonstrated through the electrodeposition of the chitosan membranes with various shapes/sizes and entrapping different enzymes. As a model experiment, glucose and ethanol were simultaneously detected in a single detection chamber without cross-talk using shape-coded chitosan membranes entrapped with glucose oxidase (GOX), peroxidase (POD), and Amplex Red (AmR) or alcohol oxidase (AOX), POD, and AmR by using same fluorescence indicator (AmR). PMID:23959236

Light-addressed electrodeposition of enzyme-entrapped chitosan membranes for multiplexed enzyme-based bioassays using a digital micromirror device.

PubMed

Huang, Shih-Hao; Wei, Lu-Shiuan; Chu, Hsiao-Tzu; Jiang, Yeu-Long

2013-08-16

This paper describes a light-addressed electrolytic system used to perform an electrodeposition of enzyme-entrapped chitosan membranes for multiplexed enzyme-based bioassays using a digital micromirror device (DMD). In this system, a patterned light illumination is projected onto a photoconductive substrate serving as a photo-cathode to electrolytically produce hydroxide ions, which leads to an increased pH gradient. The high pH generated at the cathode can cause a local gelation of chitosan through sol-gel transition. By controlling the illumination pattern on the DMD, a light-addressed electrodeposition of chitosan membranes with different shapes and sizes, as well as multiplexed micropatterning, was performed. The effect of the illumination time of the light pattern on the dimensional resolution of chitosan membrane formation was examined experimentally. Moreover, multiplexed enzyme-based bioassay of enzyme-entrapped chitosan membranes was also successfully demonstrated through the electrodeposition of the chitosan membranes with various shapes/sizes and entrapping different enzymes. As a model experiment, glucose and ethanol were simultaneously detected in a single detection chamber without cross-talk using shape-coded chitosan membranes entrapped with glucose oxidase (GOX), peroxidase (POD), and Amplex Red (AmR) or alcohol oxidase (AOX), POD, and AmR by using same fluorescence indicator (AmR).

Frequency Division Multiplexing of Interferometric Sensor Arrays

DTIC Science & Technology

1989-05-03

exception to this is the approach which employs Fabry - Perot sensorsg 10,12 in which higher order reflections will result inmoderately severe crosstalk...The Fabry - Perot technique appears to have limited array applications because of this problem. Although frequency division multiplexing has received...interferometers (- 4 cm path difference) and phase generated carrier demultiplexing demodulation . This approach leads to a simple all-passive sensor

UGV Interoperability Profile (IOP) Communications Profile, Version 0

DTIC Science & Technology

2011-12-21

some UGV systems employ Orthogonal Frequency Division Multiplexing ( OFDM ) or Coded Orthogonal Frequency Division Multiplexing (COFDM) waveforms which...other portions of the IOP. Attribute Paragraph Title Values Waveform 3.3 Air Interface/ Waveform OFDM , COFDM, DDL, CDL, None OCU to Platform...Sight MANET Mobile Ad-hoc Network Mbps Megabits per second MC/PM Master Controller/ Payload Manager MHz Megahertz MIMO Multiple Input Multiple

3D motion picture of transparent gas flow by parallel phase-shifting digital holography

NASA Astrophysics Data System (ADS)

Awatsuji, Yasuhiro; Fukuda, Takahito; Wang, Yexin; Xia, Peng; Kakue, Takashi; Nishio, Kenzo; Matoba, Osamu

2018-03-01

Parallel phase-shifting digital holography is a technique capable of recording three-dimensional (3D) motion picture of dynamic object, quantitatively. This technique can record single hologram of an object with an image sensor having a phase-shift array device and reconstructs the instantaneous 3D image of the object with a computer. In this technique, a single hologram in which the multiple holograms required for phase-shifting digital holography are multiplexed by using space-division multiplexing technique pixel by pixel. We demonstrate 3D motion picture of dynamic and transparent gas flow recorded and reconstructed by the technique. A compressed air duster was used to generate the gas flow. A motion picture of the hologram of the gas flow was recorded at 180,000 frames/s by parallel phase-shifting digital holography. The phase motion picture of the gas flow was reconstructed from the motion picture of the hologram. The Abel inversion was applied to the phase motion picture and then the 3D motion picture of the gas flow was obtained.

Single-sideband modulator for frequency domain multiplexing of superconducting qubit readout

NASA Astrophysics Data System (ADS)

Chapman, Benjamin J.; Rosenthal, Eric I.; Kerckhoff, Joseph; Vale, Leila R.; Hilton, Gene C.; Lehnert, K. W.

2017-04-01

We introduce and experimentally characterize a superconducting single-sideband modulator compatible with cryogenic microwave circuits and propose its use for frequency domain multiplexing of superconducting qubit readout. The monolithic double-balanced modulators that comprise the device are formed with purely reactive elements (capacitors and Josephson junction inductors) and require no microwave-frequency control tones. Microwave signals in the 4 to 8 GHz band, with power up to -85 dBm, are converted up or down in frequency by as much as 120 MHz. Spurious harmonics in the device can be suppressed by up to 25 dB for select probe and modulation frequencies.

Multiplex pyrosequencing method to determine CYP2C9*3, VKORC1*2, and CYP4F2*3 polymorphisms simultaneously: its application to a Korean population and comparisons with other ethnic groups.

PubMed

Kim, Kyoung-Ah; Song, Wan-Geun; Lee, Hae-Mi; Joo, Hyun-Jin; Park, Ji-Young

2014-11-01

Warfarin is an anticoagulant that is difficult to administer because of the wide variation in dose requirements to achieve a therapeutic effect. CYP2C9, VKROC1, and CYP4F2 play important roles in warfarin metabolism, and their genetic polymorphisms are related to the variability in dose determination. In this study we describe a new multiplex pyrosequencing method to identify CYP2C9*3 (rs1057910), VKORC1*2 (rs9923231), and CYP4F2*3 (rs2108661) simultaneously. A multiplex pyrosequencing method to simultaneously detect CYP2C9*3, VKORC1*2, and CYP4F2*3 alleles was designed. We assessed the allele frequencies of the polymorphisms in 250 Korean subjects using the multiplex pyrosequencing method. The results showed 100 % concordance between single and multiplex pyrosequencing methods, and the polymorphisms identified by pyrosequencing were also validated with the direct sequencing method. The allele frequencies of these polymorphisms in this population were as follows: 0.040 for CYP2C9*3, 0.918 for VKORC1*2, and 0.416 for CYP4F2*3. Although the allele frequencies of the CYP2C9*3 and VKROC1*2 were comparable to those in Japanese and Chinese populations, their frequencies in this Korean population differed from those in other ethnic groups; the CYP4F2*3 frequency was the highest among other ethnic populations including Chinese and Japanese populations. The pyrosequencing methods developed were rapid and reliable for detecting CYP2C9*3, VKORC1*2, and CYP4F2*3. Large ethnic differences in the frequency of these genetic polymorphisms were noted among ethnic groups. CYP4F2*3 exhibited its highest allele frequency among other ethnic populations compared to that in a Korean population.

TES X-ray microcalorimeters for X-ray astronomy and material analysis

NASA Astrophysics Data System (ADS)

Mitsuda, Kazuhisa

2016-11-01

TES X-ray microcalorimeter arrays provide not only high-energy resolution (FWHM < 10eV) in X-ray spectroscopy but also imaging and high-counting-rate capabilities. They are very promising spectrometer for X-ray astronomy and material analysis. In this paper, we report our recent progress. For material analysis, we have fabricated 8 × 8 format array with a fast signal response ( 40 μs) and proved the energy resolution of 5.8 eV FWHM at 5.9 keV. We developed common biasing scheme to reduce number of wirings from room temperature to the cryogenic stage. From measurements using the newly-designed common-bias SQUID array amplifier chips, and from numerical simulations, we demonstrated that signal cross talks due to the common bias is enough small. For space applications, we are developing frequency-division signal multiplexing system. We have fabricated a baseband feedback system and demonstrated that the noise added by the feedback system is about 4 eV FWHM equivalent for 16 ch multiplexing system. The digital to analog converter (DAC) dominates the noise, and needs be reduced by a factor of four for future astronomy missions.

Frequency-Modulated Microwave Photonic Links with Direct Detection: Review and Theory

DTIC Science & Technology

2010-12-15

create large amounts of signal distortion. Alternatives to MZIs have been pro- posed, including Fabry - Perot interferometers, ber Bragg gratings (FBGs...multiplexed, analog signals for applications in cable television distribution. Experimental results for a Fabry - Perot discriminated, FM subcarrier...multiplexed system were presented by [17]. An array of optical frequency modulated DFB lasers and a Fabry - Perot discriminator were used to transmit and

Asynchronous updates can promote the evolution of cooperation on multiplex networks

NASA Astrophysics Data System (ADS)

Allen, James M.; Hoyle, Rebecca B.

2017-04-01

We study the importance to the frequency of cooperation of the choice of updating strategies in a game played asynchronously or synchronously across layers in a multiplex network. Updating asynchronously in the public goods game leads to higher frequencies of cooperation compared to synchronous updates. How large this effect is depends on the sensitivity of the game dynamics to changes in the number of cooperators surrounding a player, with the largest effect observed when players payoffs are small. The discovery of this effect enhances understanding of cooperation on multiplex networks, and demonstrates a new way to maintain cooperation in these systems.

Frequency-multiplexed and pipelined iterative optical systolic array processors

NASA Technical Reports Server (NTRS)

Casasent, D.; Jackson, J.; Neuman, C.

1983-01-01

Optical matrix processors using acoustooptic transducers are described, with emphasis on new systolic array architectures using frequency multiplexing in addition to space and time multiplexing. A Kalman filtering application is considered in a case study from which the operations required on such a system can be defined. This also serves as a new and powerful application for iterative optical processors. The importance of pipelining the data flow and the ordering of the operations performed in a specific application of such a system are also noted. Several examples of how to effectively achieve this are included. A new technique for handling bipolar data on such architectures is also described.

Frequency division multiplexed readout of TES detectors with baseband feedback

NASA Astrophysics Data System (ADS)

den Hartog, R.; Audley, M. D.; Beyer, J.; Bruijn, M. P.; de Korte, P.; Gottardi, L.; Hijmering, R.; Jackson, B.; Nieuwenhuizen, A.; van der Kuur, J.; van Leeuwen, B.-J.; Van Loon, D.

2012-09-01

SRON is developing an electronic system for the multiplexed read-out of an array of transition edge sensors (TES) by combining the techniques of frequency domain multiplexing (FDM) with base-band feedback (BBFB). The astronomical applications are the read-out of soft X-ray microcalorimeters and the far-infrared bolometers for the SAFARI instrument on the Japanese mission SPICA. In this paper we derive the requirements for the read-out system regarding noise and dynamic range in the context of the SAFARI instrument, and demonstrate that the current experimental prototype is capable of simultaneously locking 57 channels and complies with these requirements.

Economical Implementation of a Filter Engine in an FPGA

NASA Technical Reports Server (NTRS)

Kowalski, James E.

2009-01-01

A logic design has been conceived for a field-programmable gate array (FPGA) that would implement a complex system of multiple digital state-space filters. The main innovative aspect of this design lies in providing for reuse of parts of the FPGA hardware to perform different parts of the filter computations at different times, in such a manner as to enable the timely performance of all required computations in the face of limitations on available FPGA hardware resources. The implementation of the digital state-space filter involves matrix vector multiplications, which, in the absence of the present innovation, would ordinarily necessitate some multiplexing of vector elements and/or routing of data flows along multiple paths. The design concept calls for implementing vector registers as shift registers to simplify operand access to multipliers and accumulators, obviating both multiplexing and routing of data along multiple paths. Each vector register would be reused for different parts of a calculation. Outputs would always be drawn from the same register, and inputs would always be loaded into the same register. A simple state machine would control each filter. The output of a given filter would be passed to the next filter, accompanied by a "valid" signal, which would start the state machine of the next filter. Multiple filter modules would share a multiplication/accumulation arithmetic unit. The filter computations would be timed by use of a clock having a frequency high enough, relative to the input and output data rate, to provide enough cycles for matrix and vector arithmetic operations. This design concept could prove beneficial in numerous applications in which digital filters are used and/or vectors are multiplied by coefficient matrices. Examples of such applications include general signal processing, filtering of signals in control systems, processing of geophysical measurements, and medical imaging. For these and other applications, it could be advantageous to combine compact FPGA digital filter implementations with other application-specific logic implementations on single integrated-circuit chips. An FPGA could readily be tailored to implement a variety of filters because the filter coefficients would be loaded into memory at startup.

Full duplex dense-wavelength-division-multiplexing radio-over-fiber system transmission of 75-GHz W-band frequency multiple-input multiple-output orthogonal-frequency-division-multiplexing signals with 3×12 Gbps downstream and 6 Gbps upstream

NASA Astrophysics Data System (ADS)

Fang, Wei Jin; Huang, Xu Guang; Yang, Kai; Zhang, Xiao Min

2012-09-01

We propose and demonstrate a full duplex dense-wavelength-division-multiplexing radio-over-fiber (DWDM-ROF) system for transmitting 75-GHz W-band frequency multiple-input multiple-output orthogonal-frequency-division-multiplexing (MIMO-OFDM) signals with 12 Gbps downstream and 6 Gbps upstream. The downstream transmitting terminal is based on a three-channels sextupling-frequency scheme using an external modulation of a distributed feedback laser diode (DFB-LD) and dual drive Mach-Zehnder modulator (DD-MZM) for carrying downstream signals. MIMO-OFDM algorithms effectively compensate for impairments in the wireless link. Without using costly W-band components in the transmitter, a 12 Gbps downstream transmission system operation at 75 GHz is experimentally validated. For the downstream transmission, a power penalty of less than 3 dB was observed after a 50 km single mode fiber (SMF) and 4 m wireless transmission at a bit error rate (BER) of 3.8×10-3. For the upstream transmission, we use a commercially available 1.5 GHz bandwidth reflective semiconductor optical amplifier (RSOA) to achieve 6 Gbps upstream traffic for 16 QAM-OFDM signals. A power penalty of 3 dB was observed after a 50 km SMF transmission at a BER of 3.8×10-3. The frequency of the local oscillator is reduced due to the frequency sextupling scheme. The cost of the proposed system is largely reduced.

Digital data, composite video multiplexer and demultiplexer boards for an IBM PC/AT compatible computer

NASA Technical Reports Server (NTRS)

Smith, Dean Lance

1993-01-01

Work continued on the design of two IBM PC/AT compatible computer interface boards. The boards will permit digital data to be transmitted over a composite video channel from the Orbiter. One board combines data with a composite video signal. The other board strips the data from the video signal.

A Web-Based Visualization and Animation Platform for Digital Logic Design

ERIC Educational Resources Information Center

Shoufan, Abdulhadi; Lu, Zheng; Huss, Sorin A.

2015-01-01

This paper presents a web-based education platform for the visualization and animation of the digital logic design process. This includes the design of combinatorial circuits using logic gates, multiplexers, decoders, and look-up-tables as well as the design of finite state machines. Various configurations of finite state machines can be selected…

Direct Digital Demultiplexing of Analog TDM Signals for Cable Reduction in Ultrasound Imaging Catheters

PubMed Central

Carpenter, Thomas M.; Rashid, M. Wasequr; Ghovanloo, Maysam; Cowell, David M. J.; Freear, Steven; Degertekin, F. Levent

2016-01-01

In real-time catheter based 3D ultrasound imaging applications, gathering data from the transducer arrays is difficult as there is a restriction on cable count due to the diameter of the catheter. Although area and power hungry multiplexing circuits integrated at the catheter tip are used in some applications, these are unsuitable for use in small sized catheters for applications like intracardiac imaging. Furthermore, the length requirement for catheters and limited power available to on-chip cable drivers leads to limited signal strength at the receiver end. In this paper an alternative approach using Analog Time Division Multiplexing (TDM) is presented which addresses the cable restrictions of ultrasound catheters. A novel digital demultiplexing technique is also described which allows for a reduction in the number of analog signal processing stages required. The TDM and digital demultiplexing schemes are demonstrated for an intracardiac imaging system that would operate in the 4 MHz to 11 MHz range. A TDM integrated circuit (IC) with 8:1 multiplexer is interfaced with a fast ADC through a micro-coaxial catheter cable bundle, and processed with an FPGA RTL simulation. Input signals to the TDM IC are recovered with −40 dB crosstalk between channels on the same micro-coax, showing the feasibility of this system for ultrasound imaging applications. PMID:27116738

Multiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations.

PubMed

Zonta, Eleonora; Garlan, Fanny; Pécuchet, Nicolas; Perez-Toralla, Karla; Caen, Ouriel; Milbury, Coren; Didelot, Audrey; Fabre, Elizabeth; Blons, Hélène; Laurent-Puig, Pierre; Taly, Valérie

2016-01-01

In cancer research, the accuracy of the technology used for biomarkers detection is remarkably important. In this context, digital PCR represents a highly sensitive and reproducible method that could serve as an appropriate tool for tumor mutational status analysis. In particular, droplet-based digital PCR approaches have been developed for detection of tumor-specific mutated alleles within plasmatic circulating DNA. Such an approach calls for the development and validation of a very significant quantity of assays, which can be extremely costly and time consuming. Herein, we evaluated assays for the detection and quantification of various mutations occurring in three genes often misregulated in cancers: the epidermal growth factor receptor (EGFR), the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) and the Tumoral Protein p53 (TP53) genes. In particular, commercial competitive allele-specific TaqMan® PCR (castPCR™) technology, as well as TaqMan® and ZEN™ assays, have been evaluated for EGFR p.L858R, p.T790M, p.L861Q point mutations and in-frame deletions Del19. Specificity and sensitivity have been determined on cell lines DNA, plasmatic circulating DNA of lung cancer patients or Horizon Diagnostics Reference Standards. To show the multiplexing capabilities of this technology, several multiplex panels for EGFR (several three- and four-plexes) have been developed, offering new "ready-to-use" tests for lung cancer patients.

Optical Injection Locking of Vertical Cavity Surface-Emitting Lasers: Digital and Analog Applications

NASA Astrophysics Data System (ADS)

Parekh, Devang

With the rise of mobile (cellphones, tablets, notebooks, etc.) and broadband wireline communications (Fiber to the Home), there are increasing demands being placed on transmitters for moving data from device to device and around the world. Digital and analog fiber-optic communications have been the key technology to meet this challenge, ushering in ubiquitous Internet and cable TV over the past 20 years. At the physical layer, high-volume low-cost manufacturing of semiconductor optoelectronic devices has played an integral role in allowing for deployment of high-speed communication links. In particular, vertical cavity surface emitting lasers (VCSEL) have revolutionized short reach communications and are poised to enter more markets due to their low cost, small size, and performance. However, VCSELs have disadvantages such as limited modulation performance and large frequency chirp which limits fiber transmission speed and distance, key parameters for many fiber-optic communication systems. Optical injection locking is one method to overcome these limitations without re-engineering the VCSEL at the device level. By locking the frequency and phase of the VCSEL by the direct injection of light from another laser oscillator, improved device performance is achieved in a post-fabrication method. In this dissertation, optical injection locking of VCSELs is investigated from an applications perspective. Optical injection locking of VCSELs can be used as a pathway to reduce complexity, cost, and size of both digital and analog fiber-optic communications. On the digital front, reduction of frequency chirp via bit pattern inversion for large-signal modulation is experimentally demonstrated showing up to 10 times reduction in frequency chirp and over 90 times increase in fiber transmission distance. Based on these results, a new reflection-based interferometric model for optical injection locking was established to explain this phenomenon. On the analog side, the resonance frequency enhancement was exploited for millimeter-wave radio over fiber communications. Experimental demonstration of 4 Gb/s data transmission over 20 km of fiber and 3 m of wireless transmission at a 60 GHz carrier frequency was achieved. Additionally, optical injection of multi-transverse mode (MM) VCSELs was investigated showing record resonance frequency enhancement of > 54 GHz and 3-dB bandwidth of 38 GHz. Besides these applications, a number of other intriguing applications are also discussed, including an optoelectronic oscillator (OEO) and wavelength-division multiplexed passive optical networks (WDM-PON). Finally, the future of optical injection locking and its direction going forward will be discussed.

Cascaded multiplexed optical link on a telecommunication network for frequency dissemination.

PubMed

Lopez, Olivier; Haboucha, Adil; Kéfélian, Fabien; Jiang, Haifeng; Chanteau, Bruno; Roncin, Vincent; Chardonnet, Christian; Amy-Klein, Anne; Santarelli, Giorgio

2010-08-02

We demonstrate a cascaded optical link for ultrastable frequency dissemination comprised of two compensated links of 150 km and a repeater station. Each link includes 114 km of Internet fiber simultaneously carrying data traffic through a dense wavelength division multiplexing technology, and passes through two routing centers of the telecommunication network. The optical reference signal is inserted in and extracted from the communication network using bidirectional optical add-drop multiplexers. The repeater station operates autonomously ensuring noise compensation on the two links and the ultra-stable signal optical regeneration. The compensated link shows a fractional frequency instability of 3 x 10(-15) at one second measurement time and 5 x 10(-20) at 20 hours. This work paves the way to a wide dissemination of ultra-stable optical clock signals between distant laboratories via the Internet network.

A 32-channel fully implantable wireless neurosensor for simultaneous recording from two cortical regions.

PubMed

Aceros, Juan; Yin, Ming; Borton, David A; Patterson, William R; Nurmikko, Arto V

2011-01-01

We present a fully implantable, wireless, neurosensor for multiple-location neural interface applications. The device integrates two independent 16-channel intracortical microelectrode arrays and can simultaneously acquire 32 channels of broadband neural data from two separate cortical areas. The system-on-chip implantable sensor is built on a flexible Kapton polymer substrate and incorporates three very low power subunits: two cortical subunits connected to a common subcutaneous subunit. Each cortical subunit has an ultra-low power 16-channel preamplifier and multiplexer integrated onto a cortical microelectrode array. The subcutaneous epicranial unit has an inductively coupled power supply, two analog-to-digital converters, a low power digital controller chip, and microlaser-based infrared telemetry. The entire system is soft encapsulated with biocompatible flexible materials for in vivo applications. Broadband neural data is conditioned, amplified, and analog multiplexed by each of the cortical subunits and passed to the subcutaneous component, where it is digitized and combined with synchronization data and wirelessly transmitted transcutaneously using high speed infrared telemetry.

A multi-channel low-power system-on-chip for single-unit recording and narrowband wireless transmission of neural signal.

PubMed

Bonfanti, A; Ceravolo, M; Zambra, G; Gusmeroli, R; Spinelli, A S; Lacaita, A L; Angotzi, G N; Baranauskas, G; Fadiga, L

2010-01-01

This paper reports a multi-channel neural recording system-on-chip (SoC) with digital data compression and wireless telemetry. The circuit consists of a 16 amplifiers, an analog time division multiplexer, an 8-bit SAR AD converter, a digital signal processor (DSP) and a wireless narrowband 400-MHz binary FSK transmitter. Even though only 16 amplifiers are present in our current die version, the whole system is designed to work with 64 channels demonstrating the feasibility of a digital processing and narrowband wireless transmission of 64 neural recording channels. A digital data compression, based on the detection of action potentials and storage of correspondent waveforms, allows the use of a 1.25-Mbit/s binary FSK wireless transmission. This moderate bit-rate and a low frequency deviation, Manchester-coded modulation are crucial for exploiting a narrowband wireless link and an efficient embeddable antenna. The chip is realized in a 0.35- εm CMOS process with a power consumption of 105 εW per channel (269 εW per channel with an extended transmission range of 4 m) and an area of 3.1 × 2.7 mm(2). The transmitted signal is captured by a digital TV tuner and demodulated by a wideband phase-locked loop (PLL), and then sent to a PC via an FPGA module. The system has been tested for electrical specifications and its functionality verified in in-vivo neural recording experiments.

Frequency-Modulated Continuous Flow Analysis Electrospray Ionization Mass Spectrometry (FM-CFA-ESI-MS) for Sample Multiplexing.

PubMed

Filla, Robert T; Schrell, Adrian M; Coulton, John B; Edwards, James L; Roper, Michael G

2018-02-20

A method for multiplexed sample analysis by mass spectrometry without the need for chemical tagging is presented. In this new method, each sample is pulsed at unique frequencies, mixed, and delivered to the mass spectrometer while maintaining a constant total flow rate. Reconstructed ion currents are then a time-dependent signal consisting of the sum of the ion currents from the various samples. Spectral deconvolution of each reconstructed ion current reveals the identity of each sample, encoded by its unique frequency, and its concentration encoded by the peak height in the frequency domain. This technique is different from other approaches that have been described, which have used modulation techniques to increase the signal-to-noise ratio of a single sample. As proof of concept of this new method, two samples containing up to 9 analytes were multiplexed. The linear dynamic range of the calibration curve was increased with extended acquisition times of the experiment and longer oscillation periods of the samples. Because of the combination of the samples, salt had little effect on the ability of this method to achieve relative quantitation. Continued development of this method is expected to allow for increased numbers of samples that can be multiplexed.

Accurate Time/Frequency Transfer Method Using Bi-Directional WDM Transmission

NASA Technical Reports Server (NTRS)

Imaoka, Atsushi; Kihara, Masami

1996-01-01

An accurate time transfer method is proposed using b-directional wavelength division multiplexing (WDM) signal transmission along a single optical fiber. This method will be used in digital telecommunication networks and yield a time synchronization accuracy of better than 1 ns for long transmission lines over several tens of kilometers. The method can accurately measure the difference in delay between two wavelength signals caused by the chromatic dispersion of the fiber in conventional simple bi-directional dual-wavelength frequency transfer methods. We describe the characteristics of this difference in delay and then show that the accuracy of the delay measurements can be obtained below 0.1 ns by transmitting 156 Mb/s times reference signals of 1.31 micrometer and 1.55 micrometers along a 50 km fiber using the proposed method. The sub-nanosecond delay measurement using the simple bi-directional dual-wavelength transmission along a 100 km fiber with a wavelength spacing of 1 nm in the 1.55 micrometer range is also shown.

Lithographed Superconducting Resonator Development for Next-Generation Frequency Multiplexing Readout of Transition-Edge Sensors

NASA Astrophysics Data System (ADS)

Faramarzi, F.; De Haan, T.; Kusaka, A.; Lee, A.; Neuhauser, B.; Plambeck, R.; Raum, C.; Suzuki, A.; Westbrook, B.

2018-03-01

Ground-based cosmic microwave background (CMB) experiments are undergoing a period of exponential growth. Current experiments are observing with 1000-10,000 detectors, and the next-generation experiment (CMB stage 4) is proposing to deploy approximately 500,000 detectors. This order of magnitude increase in detector count will require a new approach for readout electronics. We have developed superconducting resonators for next-generation frequency-domain multiplexing (fMUX) readout architecture. Our goal is to reduce the physical size of resonators, such that resonators and detectors can eventually be integrated on a single wafer. To reduce the size of these resonators, we have designed spiral inductors and interdigitated capacitors that resonate around 10-100 MHz, an order of magnitude higher frequency compared to current fMUX readout systems. The higher frequency leads to a wider bandwidth and would enable higher multiplexing factor than the current ˜ 50 detectors per readout channel. We will report on the simulation, fabrication method, characterization technique, and measurement of quality factor of these resonators.

Developments of Highly Multiplexed, Multi-chroic Pixels for Balloon-Borne Platforms

NASA Astrophysics Data System (ADS)

Aubin, F.; Hanany, S.; Johnson, B. R.; Lee, A.; Suzuki, A.; Westbrook, B.; Young, K.

2018-02-01

We present our work to develop and characterize low thermal conductance bolometers that are part of sinuous antenna multi-chroic pixels (SAMP). We use longer, thinner and meandered bolometer legs to achieve 9 pW/K thermal conductance bolometers. We also discuss the development of inductor-capacitor chips operated at 4 K to extend the multiplexing factor of the frequency domain multiplexing to 105, an increase of 60% compared to the factor currently demonstrated for this readout system. This technology development is motivated by EBEX-IDS, a balloon-borne polarimeter designed to characterize the polarization of foregrounds and to detect the primordial gravity waves through their B-mode signature on the polarization of the cosmic microwave background. EBEX-IDS will operate 20,562 transition edge sensor bolometers spread over 7 frequency bands between 150 and 360 GHz. Balloon and satellite platforms enable observations at frequencies inaccessible from the ground and with higher instantaneous sensitivity. This development improves the readiness of the SAMP and frequency domain readout technologies for future satellite applications.

A highly addressable static droplet array enabling digital control of a single droplet at pico-volume resolution.

PubMed

Jeong, Heon-Ho; Lee, Byungjin; Jin, Si Hyung; Jeong, Seong-Geun; Lee, Chang-Soo

2016-04-26

Droplet-based microfluidics enabling exquisite liquid-handling has been developed for diagnosis, drug discovery and quantitative biology. Compartmentalization of samples into a large number of tiny droplets is a great approach to perform multiplex assays and to improve reliability and accuracy using a limited volume of samples. Despite significant advances in microfluidic technology, individual droplet handling in pico-volume resolution is still a challenge in obtaining more efficient and varying multiplex assays. We present a highly addressable static droplet array (SDA) enabling individual digital manipulation of a single droplet using a microvalve system. In a conventional single-layer microvalve system, the number of microvalves required is dictated by the number of operation objects; thus, individual trap-and-release on a large-scale 2D array format is highly challenging. By integrating double-layer microvalves, we achieve a "balloon" valve that preserves the pressure-on state under released pressure; this valve can allow the selective releasing and trapping of 7200 multiplexed pico-droplets using only 1 μL of sample without volume loss. This selectivity and addressability completely arranged only single-cell encapsulated droplets from a mixture of droplet compositions via repetitive selective trapping and releasing. Thus, it will be useful for efficient handling of miniscule volumes of rare or clinical samples in multiplex or combinatory assays, and the selective collection of samples.

Microwave SQUID Multiplexer for the Readout of Metallic Magnetic Calorimeters

NASA Astrophysics Data System (ADS)

Kempf, S.; Gastaldo, L.; Fleischmann, A.; Enss, C.

2014-06-01

We have realized a frequency-domain multiplexing technique for the readout of large metallic magnetic calorimeter detector arrays. It is based on non-hysteretic single-junction SQUIDs and allows for a simultaneous readout of hundreds or thousands of detectors by using a single cryogenic high electron mobility transistor amplifier and two coaxial cables that are routed from room-temperature to the detector array. We discuss the working principle of the multiplexer and present details about our prototype multiplexer design. We show that fabricated devices are fully operational and that characteristic SQUID parameters such as the input sensitivity of the SQUID or the resonance frequency of the readout circuit can be predicted with confidence. Our best device so far has shown a magnetic flux white noise level of 1.4 m which can in future be reduced by an optimization of the fabrication processes as well as an improved microwave readout system.

Security analysis of orthogonal-frequency-division-multiplexing-based continuous-variable quantum key distribution with imperfect modulation

NASA Astrophysics Data System (ADS)

Zhang, Hang; Mao, Yu; Huang, Duan; Li, Jiawei; Zhang, Ling; Guo, Ying

2018-05-01

We introduce a reliable scheme for continuous-variable quantum key distribution (CV-QKD) by using orthogonal frequency division multiplexing (OFDM). As a spectrally efficient multiplexing technique, OFDM allows a large number of closely spaced orthogonal subcarrier signals used to carry data on several parallel data streams or channels. We place emphasis on modulator impairments which would inevitably arise in the OFDM system and analyze how these impairments affect the OFDM-based CV-QKD system. Moreover, we also evaluate the security in the asymptotic limit and the Pirandola-Laurenza-Ottaviani-Banchi upper bound. Results indicate that although the emergence of imperfect modulation would bring about a slight decrease in the secret key bit rate of each subcarrier, the multiplexing technique combined with CV-QKD results in a desirable improvement on the total secret key bit rate which can raise the numerical value about an order of magnitude.

Time-domain multiplexed high resolution fiber optics strain sensor system based on temporal response of fiber Fabry-Perot interferometers.

PubMed

Chen, Jiageng; Liu, Qingwen; He, Zuyuan

2017-09-04

We developed a multiplexed strain sensor system with high resolution using fiber Fabry-Perot interferometers (FFPI) as sensing elements. The temporal responses of the FFPIs excited by rectangular laser pulses are used to obtain the strain applied on each FFPI. The FFPIs are connected by cascaded couplers and delay fiber rolls for the time-domain multiplexing. A compact optoelectronic system performing closed-loop cyclic interrogation is employed to improve the sensing resolution and the frequency response. In the demonstration experiment, 3-channel strain sensing with resolutions better than 0.1 nε and frequency response higher than 100 Hz is realized.

Ultra-high throughput real-time instruments for capturing fast signals and rare events

NASA Astrophysics Data System (ADS)

Buckley, Brandon Walter

Wide-band signals play important roles in the most exciting areas of science, engineering, and medicine. To keep up with the demands of exploding internet traffic, modern data centers and communication networks are employing increasingly faster data rates. Wide-band techniques such as pulsed radar jamming and spread spectrum frequency hopping are used on the battlefield to wrestle control of the electromagnetic spectrum. Neurons communicate with each other using transient action potentials that last for only milliseconds at a time. And in the search for rare cells, biologists flow large populations of cells single file down microfluidic channels, interrogating them one-by-one, tens of thousands of times per second. Studying and enabling such high-speed phenomena pose enormous technical challenges. For one, parasitic capacitance inherent in analog electrical components limits their response time. Additionally, converting these fast analog signals to the digital domain requires enormous sampling speeds, which can lead to significant jitter and distortion. State-of-the-art imaging technologies, essential for studying biological dynamics and cells in flow, are limited in speed and sensitivity by finite charge transfer and read rates, and by the small numbers of photo-electrons accumulated in short integration times. And finally, ultra-high throughput real-time digital processing is required at the backend to analyze the streaming data. In this thesis, I discuss my work in developing real-time instruments, employing ultrafast optical techniques, which overcome some of these obstacles. In particular, I use broadband dispersive optics to slow down fast signals to speeds accessible to high-bit depth digitizers and signal processors. I also apply telecommunication multiplexing techniques to boost the speeds of confocal fluorescence microscopy. The photonic time stretcher (TiSER) uses dispersive Fourier transformation to slow down analog signals before digitization and processing. The act of time-stretching effectively boosts the performance of the back-end electronics and digital signal processors. The slowed down signals reach the back-end electronics with reduced bandwidth, and are therefore less affected by high-frequency roll-off and distortion. Time-stretching also increases the effective sampling rate of analog-to-digital converters and reduces aperture jitter, thereby improving resolution. Finally, the instantaneous throughputs of digital signal processors are enhanced by the stretch factor to otherwise unattainable speeds. Leveraging these unique capabilities, TiSER becomes the ideal tool for capturing high-speed signals and characterizing rare phenomena. For this thesis, I have developed techniques to improve the spectral efficiency, bandwidth, and resolution of TiSER using polarization multiplexing, all-optical modulation, and coherent dispersive Fourier transformation. To reduce the latency and improve the data handling capacity, I have also designed and implemented a real-time digital signal processing electronic backend, achieving 1.5 tera-bit per second instantaneous processing throughput. Finally, I will present results from experiments highlighting TiSER's impact in real-world applications. Confocal fluorescence microscopy is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is problematic for acquiring blur-free images of fast phenomena and cells flowing at high speed. Here I introduce a new fluorescence imaging modality, which leverages techniques from wireless communication to reach record pixel and frame rates. Termed Fluorescence Imaging using Radio-frequency tagged Emission (FIRE), this new imaging modality is capable of resolving never before seen dynamics in living cells - such as action potentials in neurons and metabolic waves in astrocytes - as well as performing high-content image assays of cells and particles in high-speed flow.

[A review of mixed gas detection system based on infrared spectroscopic technique].

PubMed

Dang, Jing-Min; Fu, Li; Yan, Zi-Hui; Zheng, Chuan-Tao; Chang, Yu-Chun; Chen, Chen; Wang, Yi-Din

2014-10-01

In order to provide the experiences and references to the researchers who are working on infrared (IR) mixed gas detection field. The proposed manuscript reviews two sections of the aforementioned field, including optical multiplexing structure and detection method. At present, the coherent light sources whose representative are quantum cascade laser (QCL) and inter-band cascade laser(ICL) become the mainstream light source in IR mixed gas detection, which replace the traditional non-coherent light source, such as IR radiation source and IR light emitting diode. In addition, the photon detector which has a super high detectivity and very short response time is gradually beyond thermal infrared detector, dominant in the field of infrared detector. The optical multiplexing structure is the key factor of IR mixed gas detection system, which consists of single light source multi-plexing detection structure and multi light source multiplexing detection structure. Particularly, single light source multiplexing detection structure is advantages of small volume and high integration, which make it a plausible candidate for the portable mixed gas detection system; Meanwhile, multi light source multiplexing detection structure is embodiment of time division multiplex, frequency division multiplexing and wavelength division multiplexing, and become the leading structure of the mixed gas detection system because of its wider spectral range, higher spectral resolution, etc. The detection method applied to IR mixed gas detection includes non-dispersive infrared (NDIR) spectroscopy, wavelength and frequency-modulation spectroscopy, cavity-enhanced spectroscopy and photoacoustic spectroscopy, etc. The IR mixed gas detection system designed by researchers after recognizing the whole sections of the proposed system, which play a significant role in industrial and agricultural production, environmental monitoring, and life science, etc.

Doubling transmission capacity in optical wireless system by antenna horizontal- and vertical-polarization multiplexing.

PubMed

Li, Xinying; Yu, Jianjun; Zhang, Junwen; Dong, Ze; Chi, Nan

2013-06-15

We experimentally demonstrate 2×56 Gb/s two-channel polarization-division-multiplexing quadrature-phase-shift-keying signal delivery over 80 km single-mode fiber-28 and 2 m Q-band (33-50 GHz) wireless link, adopting antenna horizontal- (H-) and vertical-polarization (V-polarization) multiplexing. At the wireless receiver, classic constant-modulus-algorithm equalization based on digital signal processing can realize polarization demultiplexing and remove the crosstalk at the same antenna polarization. By adopting antenna polarization multiplexing, the signal baud rate and performance requirements for optical and wireless devices can be reduced but at the cost of double antennas and devices, while wireless transmission capacity can also be increased but at the cost of stricter requirements for V-polarization. The isolation is only about 19 dB when V-polarization deviation approaches 10°, which will affect high-speed (>50 Gb/s) wireless delivery.

Thirty-Four Megabit Four-Channel Multiplexer

DTIC Science & Technology

1985-10-01

person or conveying any rights or permis- sion to manufacture , use, or sell any patented invention thai ma> in any way be related thereto. WARNING...System (DCS) in Europe. The system is evolving from frequency division multiplex ( FDM ) to time division multiplex (TDM) equipment. The bulk of the...channel multi- plexer permits expansion by providing the spare MBS and 2.048~Mb/s data channels, and will do so with no additional cost

Wavelength-multiplexed fiber optic position encoder for aircraft control systems

NASA Astrophysics Data System (ADS)

Beheim, Glenn; Krasowski, Michael J.; Sotomayor, Jorge L.; Fritsch, Klaus; Flatico, Joseph M.; Bathurst, Richard L.; Eustace, John G.; Anthan, Donald J.

1991-02-01

NASA Lewis together with John Carroll University has worked for the last several years to develop wavelength-multiplexed digital position transducers for use in aircraft control systems. A prototype rotary encoder is being built for a demonstration program involving the control of a commercial transport''s turbofan engine. This encoder has eight bits of resolution a 90 degree range and is powered by a single LED. A compact electro-optics module is being developed to withstand the extremely hostile gas turbine environment.

Multiplex picoliter-droplet digital PCR for quantitative assessment of EGFR mutations in circulating cell-free DNA derived from advanced non-small cell lung cancer patients.

PubMed

Yu, Qian; Huang, Fei; Zhang, Meilin; Ji, Haiying; Wu, Shenchao; Zhao, Ying; Zhang, Chunyan; Wu, Jiong; Wang, Beili; Pan, Baisheng; Zhang, Xin; Guo, Wei

2017-08-01

To explore the possible diagnostic value of liquid biopsy, two multiplex panels using picoliter-droplet digital polymerase chain reaction (ddPCR) were established to quantitatively assess the epidermal growth factor receptor (EGFR) mutations in cell‑free DNA (cfDNA) extracted from the plasma of advanced non‑small cell lung cancer (NSCLC) patients. Plasma samples derived from 22 patients with stage IIIB/IV NSCLC harboring EGFR mutations in matched tumor tissues confirmed by amplification refractory mutation system (ARMS) analysis were subjected to two multiplex ddPCR panels to assess the abundance of tyrosine kinase inhibitor (TKI) ‑sensitive (19DEL, L858R) and TKI‑resistant (T790 M) mutations. Fluctuations in EGFR mutant abundance were monitored by either of the multiplex ddPCR panels for three patients undergoing EGFR‑TKI treatment, with serial plasma sample collections over 2 months. The multiplex ddPCR panels applied to plasma cfDNA from advanced NSCLC patients achieved a total concordance rate of 80% with the EGFR mutation profiles obtained by ARMS from matched biopsy tumor specimens (90% for 19DEL, 95% for L858R, 95% for T790M, respectively) and revealed additional mutant alleles in two subjects. The respective sensitivity and specificity were 90.9 and 88.9% for 19DEL, 87.5 and 100% for L858R, 100 and 93.8% for T790M. The fluctuations of EGFR mutant abundance in serial plasma cfDNA were in accordance with the changes in tumor size as assessed by imaging scans. The authors demonstrated the utility of multiplex ddPCR panels with ultra‑sensitivity for quantitative analysis of EGFR mutations in plasma cfDNA and obtained promising usefulness in EGFR‑TKI decision‑making for advanced NSCLC patients.

a Study of Multiplexing Schemes for Voice and Data.

NASA Astrophysics Data System (ADS)

Sriram, Kotikalapudi

Voice traffic variations are characterized by on/off transitions of voice calls, and talkspurt/silence transitions of speakers in conversations. A speaker is known to be in silence for more than half the time during a telephone conversation. In this dissertation, we study some schemes which exploit speaker silences for an efficient utilization of the transmission capacity in integrated voice/data multiplexing and in digital speech interpolation. We study two voice/data multiplexing schemes. In each scheme, any time slots momentarily unutilized by the voice traffic are made available to data. In the first scheme, the multiplexer does not use speech activity detectors (SAD), and hence the voice traffic variations are due to call on/off only. In the second scheme, the multiplexer detects speaker silences using SAD and transmits voice only during talkspurts. The multiplexer with SAD performs digital speech interpolation (DSI) as well as dynamic channel allocation to voice and data. The performance of the two schemes is evaluated using discrete-time modeling and analysis. The data delay performance for the case of English speech is compared with that for the case of Japanese speech. A closed form expression for the mean data message delay is derived for the single-channel single-talker case. In a DSI system, occasional speech losses occur whenever the number of speakers in simultaneous talkspurt exceeds the number of TDM voice channels. In a buffered DSI system, speech loss is further reduced at the cost of delay. We propose a novel fixed-delay buffered DSI scheme. In this scheme, speech fill-in/hangover is not required because there are no variable delays. Hence, all silences that naturally occur in speech are fully utilized. Consequently, a substantial improvement in the DSI performance is made possible. The scheme is modeled and analyzed in discrete -time. Its performance is evaluated in terms of the probability of speech clipping, packet rejection ratio, DSI advantage, and the delay.

Multiplex picoliter-droplet digital PCR for quantitative assessment of EGFR mutations in circulating cell-free DNA derived from advanced non-small cell lung cancer patients

PubMed Central

Yu, Qian; Huang, Fei; Zhang, Meilin; Ji, Haiying; Wu, Shenchao; Zhao, Ying; Zhang, Chunyan; Wu, Jiong; Wang, Beili; Pan, Baisheng; Zhang, Xin; Guo, Wei

2017-01-01

To explore the possible diagnostic value of liquid biopsy, two multiplex panels using picoliter-droplet digital polymerase chain reaction (ddPCR) were established to quantitatively assess the epidermal growth factor receptor (EGFR) mutations in cell-free DNA (cfDNA) extracted from the plasma of advanced non-small cell lung cancer (NSCLC) patients. Plasma samples derived from 22 patients with stage IIIB/IV NSCLC harboring EGFR mutations in matched tumor tissues confirmed by amplification refractory mutation system (ARMS) analysis were subjected to two multiplex ddPCR panels to assess the abundance of tyrosine kinase inhibitor (TKI) -sensitive (19DEL, L858R) and TKI-resistant (T790 M) mutations. Fluctuations in EGFR mutant abundance were monitored by either of the multiplex ddPCR panels for three patients undergoing EGFR-TKI treatment, with serial plasma sample collections over 2 months. The multiplex ddPCR panels applied to plasma cfDNA from advanced NSCLC patients achieved a total concordance rate of 80% with the EGFR mutation profiles obtained by ARMS from matched biopsy tumor specimens (90% for 19DEL, 95% for L858R, 95% for T790M, respectively) and revealed additional mutant alleles in two subjects. The respective sensitivity and specificity were 90.9 and 88.9% for 19DEL, 87.5 and 100% for L858R, 100 and 93.8% for T790M. The fluctuations of EGFR mutant abundance in serial plasma cfDNA were in accordance with the changes in tumor size as assessed by imaging scans. The authors demonstrated the utility of multiplex ddPCR panels with ultra-sensitivity for quantitative analysis of EGFR mutations in plasma cfDNA and obtained promising usefulness in EGFR-TKI decision-making for advanced NSCLC patients. PMID:29067441

47 CFR 76.602 - Incorporation by reference.

Code of Federal Regulations, 2013 CFR

2013-10-01

... MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Technical Standards § 76.602 Incorporation by reference. (a....640. (5) ANSI/SCTE 54 2003 (formerly DVS 241), “Digital Video Service Multiplex and Transport System...

Kramers-Kronig receiver operable without digital upsampling.

PubMed

Bo, Tianwai; Kim, Hoon

2018-05-28

The Kramers-Kronig (KK) receiver is capable of retrieving the phase information of optical single-sideband (SSB) signal from the optical intensity when the optical signal satisfies the minimum phase condition. Thus, it is possible to direct-detect the optical SSB signal without suffering from the signal-signal beat interference and linear transmission impairments. However, due to the spectral broadening induced by nonlinear operations in the conventional KK algorithm, it is necessary to employ the digital upsampling at the beginning of the digital signal processing (DSP). The increased number of samples at the DSP would hinder the real-time implementation of this attractive receiver. Hence, we propose a new DSP algorithm for KK receiver operable at 2 samples per symbol. We adopt a couple of mathematical approximations to avoid the use of nonlinear operations such as logarithm and exponential functions. By using the proposed algorithm, we demonstrate the transmission of 112-Gb/s SSB orthogonal frequency-division-multiplexed signal over an 80-km fiber link. The results show that the proposed algorithm operating at 2 samples per symbol exhibits similar performance to the conventional KK one operating at 6 samples per symbol. We also present the error analysis of the proposed algorithm for KK receiver in comparison with the conventional one.

A rocket-borne pulse-height analyzer for energetic particle measurements

NASA Technical Reports Server (NTRS)

Leung, W.; Smith, L. G.; Voss, H. D.

1979-01-01

The pulse-height analyzer basically resembles a time-sharing multiplexing data-acquisition system which acquires analog data (from energetic particle spectrometers) and converts them into digital code. The PHA simultaneously acquires pulse-height information from the analog signals of the four input channels and sequentially multiplexes the digitized data to a microprocessor. The PHA together with the microprocessor form an on-board real-time data-manipulation system. The system processes data obtained during the rocket flight and reduces the amount of data to be sent back to the ground station. Consequently the data-reduction process for the rocket experiments is speeded up. By using a time-sharing technique, the throughput rate of the microprocessor is increased. Moreover, data from several particle spectrometers are manipulated to share one information channel; consequently, the TM capacity is increased.

Multiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations

PubMed Central

Zonta, Eleonora; Garlan, Fanny; Pécuchet, Nicolas; Perez-Toralla, Karla; Caen, Ouriel; Milbury, Coren; Didelot, Audrey; Fabre, Elizabeth; Blons, Hélène; Laurent-Puig, Pierre; Taly, Valérie

2016-01-01

In cancer research, the accuracy of the technology used for biomarkers detection is remarkably important. In this context, digital PCR represents a highly sensitive and reproducible method that could serve as an appropriate tool for tumor mutational status analysis. In particular, droplet-based digital PCR approaches have been developed for detection of tumor-specific mutated alleles within plasmatic circulating DNA. Such an approach calls for the development and validation of a very significant quantity of assays, which can be extremely costly and time consuming. Herein, we evaluated assays for the detection and quantification of various mutations occurring in three genes often misregulated in cancers: the epidermal growth factor receptor (EGFR), the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) and the Tumoral Protein p53 (TP53) genes. In particular, commercial competitive allele-specific TaqMan® PCR (castPCR™) technology, as well as TaqMan® and ZEN™ assays, have been evaluated for EGFR p.L858R, p.T790M, p.L861Q point mutations and in-frame deletions Del19. Specificity and sensitivity have been determined on cell lines DNA, plasmatic circulating DNA of lung cancer patients or Horizon Diagnostics Reference Standards. To show the multiplexing capabilities of this technology, several multiplex panels for EGFR (several three- and four-plexes) have been developed, offering new "ready-to-use" tests for lung cancer patients. PMID:27416070

Multiplexing Readout of TES Microcalorimeters Based on Analog Baseband Feedback

DOE Office of Scientific and Technical Information (OSTI.GOV)

Takei, Y.; Yamasaki, N.Y; Mitsuda, K.

2009-12-16

A TES microcalorimeter array is a promising spectrometer with excellent energy resolution and a moderate imaging capability. To realize a large format array in space, multiplexing the TES signals at the low tempersture stage is mandatory. We are developing frequency division multiplexing (FDM) based on baseband feedback technique. In FDM, each TES is AC-biased with a different carrier frequency. Signals from several pixels are summed and then read out by one SQUID. The maximum number of multiplexed pixels are limited by the frequency band in which the SQUID can be operated in a flux-locked loop, which is {approx}1 MHz withmore » standard flux-locked loop circuit. In the baseband feedback, the signal ({approx}10 kHz band) from the TES is once demodulated. Then a reconstructed copy of the modulated signal with an appropriate phase is fed back to the SQUID input coil to maintain an approximately constant magnetic flux. This can be implemented even for large cable delays and automatically suppresses the carrier. We developed a prototype electronics for the baseband feedback based on an analog phase sensitive detector (PSD) and a multiplier. Combined with Seiko 80-SSA SQUID amp, open-loop gain of 8 has been obtained for 10 kHz baseband signal at 5 MHz carrier frequency, with a moderate noise contribution of 27pA/{radical}(Hz) at input.« less

ddPCRclust - An R package and Shiny app for automated analysis of multiplexed ddPCR data.

PubMed

Brink, Benedikt G; Meskas, Justin; Brinkman, Ryan R

2018-03-09

Droplet digital PCR (ddPCR) is an emerging technology for quantifying DNA. By partitioning the target DNA into ∼20000 droplets, each serving as its own PCR reaction compartment, a very high sensitivity of DNA quantification can be achieved. However, manual analysis of the data is time consuming and algorithms for automated analysis of non-orthogonal, multiplexed ddPCR data are unavailable, presenting a major bottleneck for the advancement of ddPCR transitioning from low-throughput to high- throughput. ddPCRclust is an R package for automated analysis of data from Bio-Rad's droplet digital PCR systems (QX100 and QX200). It can automatically analyse and visualise multiplexed ddPCR experiments with up to four targets per reaction. Results are on par with manual analysis, but only take minutes to compute instead of hours. The accompanying Shiny app ddPCRvis provides easy access to the functionalities of ddPCRclust through a web-browser based GUI. R package: https://github.com/bgbrink/ddPCRclust; Interface: https://github.com/bgbrink/ddPCRvis/; Web: https://bibiserv.cebitec.uni-bielefeld.de/ddPCRvis/. bbrink@cebitec.uni-bielefeld.de.

Demosaiced pixel super-resolution for multiplexed holographic color imaging

PubMed Central

Wu, Yichen; Zhang, Yibo; Luo, Wei; Ozcan, Aydogan

2016-01-01

To synthesize a holographic color image, one can sequentially take three holograms at different wavelengths, e.g., at red (R), green (G) and blue (B) parts of the spectrum, and digitally merge them. To speed up the imaging process by a factor of three, a Bayer color sensor-chip can also be used to demultiplex three wavelengths that simultaneously illuminate the sample and digitally retrieve individual set of holograms using the known transmission spectra of the Bayer color filters. However, because the pixels of different channels (R, G, B) on a Bayer color sensor are not at the same physical location, conventional demosaicing techniques generate color artifacts in holographic imaging using simultaneous multi-wavelength illumination. Here we demonstrate that pixel super-resolution can be merged into the color de-multiplexing process to significantly suppress the artifacts in wavelength-multiplexed holographic color imaging. This new approach, termed Demosaiced Pixel Super-Resolution (D-PSR), generates color images that are similar in performance to sequential illumination at three wavelengths, and therefore improves the speed of holographic color imaging by 3-fold. D-PSR method is broadly applicable to holographic microscopy applications, where high-resolution imaging and multi-wavelength illumination are desired. PMID:27353242

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR

PubMed Central

Schrell, Adrian M.; Roper, Michael G.

2014-01-01

A frequency-modulated fluorescence encoding method was used as a means to increase the number of fluorophores monitored during infrared-mediated polymerase chain reaction. Laser lines at 488-nm and 561-nm were modulated at 73- and 137-Hz, respectively, exciting fluorescence from the dsDNA intercalating dye, EvaGreen, and the temperature insensitive dye, ROX. Emission was collected in a color-blind manner using a single photomultiplier tube for detection and demodulated by frequency analysis. The resulting frequency domain signal resolved the contribution from the two fluorophores as well as the background from the IR lamp. The detection method was successfully used to measure amplification of DNA samples containing 104 – 107 starting copies of template producing an amplification efficiency of 96%. The utility of this methodology was further demonstrated by simultaneous amplification of two genes from human genomic DNA using different color TaqMan probes. This method of multiplexing fluorescence detection with IR-qPCR is ideally suited as it allowed isolation of the signals of interest from the background in the frequency domain and is expected to further reduce the complexity of multiplexed microfluidic IR-qPCR instrumentation. PMID:24448431

Pilot-multiplexed continuous-variable quantum key distribution with a real local oscillator

NASA Astrophysics Data System (ADS)

Wang, Tao; Huang, Peng; Zhou, Yingming; Liu, Weiqi; Zeng, Guihua

2018-01-01

We propose a pilot-multiplexed continuous-variable quantum key distribution (CVQKD) scheme based on a local local oscillator (LLO). Our scheme utilizes time-multiplexing and polarization-multiplexing techniques to dramatically isolate the quantum signal from the pilot, employs two heterodyne detectors to separately detect the signal and the pilot, and adopts a phase compensation method to almost eliminate the multifrequency phase jitter. In order to analyze the performance of our scheme, a general LLO noise model is constructed. Besides the phase noise and the modulation noise, the photon-leakage noise from the reference path and the quantization noise due to the analog-to-digital converter (ADC) are also considered, which are first analyzed in the LLO regime. Under such general noise model, our scheme has a higher key rate and longer secure distance compared with the preexisting LLO schemes. Moreover, we also conduct an experiment to verify our pilot-multiplexed scheme. Results show that it maintains a low level of the phase noise and is expected to obtain a 554-Kbps secure key rate within a 15-km distance under the finite-size effect.

Prototype data terminal-multiplexer/demultiplexer

NASA Technical Reports Server (NTRS)

Leck, D. E.; Goodwin, J. E.

1972-01-01

The design and operation of a quad redundant data terminal and a multiplexer/demultiplexer (MDU) is described. The most unique feature is the design of the quad redundant data terminal. This is one of the few designs where the unit is fail/op, fail/op, fail/safe. Laboratory tests confirm that the unit will operate satisfactorily with the failure of three out of four channels. Although the design utilizes state-of-the-art technology, the waveform error checks, the voting techniques, and the parity bit checks are believed to be used in unique configurations. Correct word selection routines are also novel. The MDU design, while not redundant, utilizes, the latest state-of-the-art advantages of light coupler and interested amplifiers. Much of the technology employed was an evolution of prior NASA contracts related to the Addressable Time Division Data System. A good example of the earlier technology development was the development of a low level analog multiplexer, a high level analog multiplexer, and a digital multiplexer. A list of all drawings is included for reference and all schematic, block and timing diagrams are incorporated.

Infrared Avionics Signal Distribution using Wavelength Division Multiplexing

NASA Technical Reports Server (NTRS)

Atiquzzaman, Mohammed; Sluss, Jim; Nguyen, Hung; Ngo, Duc

2003-01-01

Pilots in the cockpits of aircrafts currently communicate with ground stations using Radio Frequency (RF) signals. Antennas mounted outside the aircraft receive and transmit RF signals from and to the ground stations. The RF signals received at the antennas are sent to the cockpit using coaxial cables. As the number of antennas needed to provide more than one frequency band in aircrafts increases, RF distribution media (such as coaxial cable) adds to the complexity and weight of the cockpit wiring. Concomitantly, the safety and signal to noise ratio also decreases due to the use of RF signals. The University of Oklahoma is collaborating with the National Aeronautics and Space Administration to develop optical fiber based schemes to replace the coaxial cable used for RF signal distribution within an aircraft. The project aims at exploiting emerging Wavelength Division Multiplexing (WDM) techniques to reduce the weight of cabling, and increase the signal to noise ratio and reliability. This will be achieved by wavelength division multiplexing the signals from the various antennas and then demultiplexing the signals to recover the original signals at the cockpit. This paper will show that (i) RF signals can not only be wavelength multiplexed at the end of a fiber, but additional signals can be inserted into the middle of the fiber using WDM technology, and (ii) the signals can also be successfully extracted by tapping into the middle of the fiber. We are currently extending our previous laboratory prototype (which could multiplex signals only at the end of the fiber) to include additional multiplexing and demultiplexing of RF signals from the middle of the optical backbone with a view to validating the proof of concept, and carrying out measurements to determine the effectiveness of Wavelength Division Multiplexing for avionics applications. A test bed to perform measurements of several relevant parameters for various modulation schemes and frequencies (such as VHF, UHF, and L-Band) has been implemented. In particular, results of transmitter and receiver noise, bit-error-rate (BER), effect of cross talk on the quality of the multiplexed signals, and Signal to Noise ratio and Carrier to Noise ratio, obtained using the aforementioned test bed, will be presented.

Multi-functional angiographic OFDI using frequency-multiplexed dual-beam illumination

PubMed Central

Kim, SunHee; Park, Taejin; Jang, Sun-Joo; Nam, Ahhyun S.; Vakoc, Benjamin J.; Oh, Wang-Yuhl

2015-01-01

Detection of blood flow inside the tissue sample can be achieved by measuring the local change of complex signal over time in angiographic optical coherence tomography (OCT). In conventional angiographic OCT, the transverse displacement of the imaging beam during the time interval between a pair of OCT signal measurements must be significantly reduced to minimize the noise due to the beam scanning-induced phase decorrelation at the expense of the imaging speed. Recent introduction of dual-beam scan method either using polarization encoding or two identical imaging systems in spectral-domain (SD) OCT scheme shows potential for high-sensitivity vasculature imaging without suffering from spurious phase noise caused by the beam scanning-induced spatial decorrelation. In this paper, we present multi-functional angiographic optical frequency domain imaging (OFDI) using frequency-multiplexed dual-beam illumination. This frequency multiplexing scheme, utilizing unique features of OFDI, provides spatially separated dual imaging beams occupying distinct electrical frequency bands that can be demultiplexed in the frequency domain processing. We demonstrate the 3D multi-functional imaging of the normal mouse skin in the dorsal skin fold chamber visualizing distinct layer structures from the intensity imaging, information about mechanical integrity from the polarization-sensitive imaging, and depth-resolved microvasculature from the angiographic imaging that are simultaneously acquired and automatically co-registered. PMID:25968731

Flexible wavelength de-multiplexer for elastic optical networking.

PubMed

Zhou, Rui; Gutierrez Pascual, M Deseada; Anandarajah, Prince M; Shao, Tong; Smyth, Frank; Barry, Liam P

2016-05-15

We report an injection locked flexible wavelength de-multiplexer (de-mux) that shows 24-h frequency stability of 1 kHz for optical comb-based elastic optical networking applications. We demonstrate 50 GHz, 87.5 GHz equal spacing and 6.25G-25G-50 GHz, 75G-50G-100 GHz unequal spacing for the de-multiplexer outputs. We also implement an unequally spaced (75G-50G-100 GHz), mixed symbol rate (12.5 GBaud and 40 GBaud) and modulation format (polarization division multiplexed quadrature phase shift keying and on-off keying) wavelength division multiplexed transmission system using the de-multiplexer outputs. The results show 0.6 dB receiver sensitivity penalty, at 7% hard decision forward error correction coding limit, of the 100 km transmitted de-mux outputs when compared to comb source seeding laser back-to-back.

Planar Lithographed Superconducting LC Resonators for Frequency-Domain Multiplexed Readout Systems

NASA Astrophysics Data System (ADS)

Rotermund, K.; Barch, B.; Chapman, S.; Hattori, K.; Lee, A.; Palaio, N.; Shirley, I.; Suzuki, A.; Tran, C.

2016-07-01

Cosmic microwave background (CMB) polarization experiments are increasing the number of transition edge sensor (TES) bolometers to increase sensitivity. In order to maintain low thermal loading of the sub-Kelvin stage, the frequency-domain multiplexing (FDM) factor has to increase accordingly. FDM is achieved by placing TES bolometers in series with inductor-capacitor (LC) resonators, which select the readout frequency. The multiplexing factor can be raised with a large total readout bandwidth and small frequency spacing between channels. The inductance is kept constant to maintain a uniform readout bandwidth across detectors, while the maximum acceptable value is determined by bolometer stability. Current technology relies on commercially available ceramic chip capacitors. These have high scatter in their capacitance thereby requiring large frequency spacing. Furthermore, they have high equivalent series resistance (ESR) at higher frequencies and are time consuming and tedious to hand assemble via soldering. A solution lies in lithographed, planar spiral inductors (currently in use by some experiments) combined with interdigitated capacitors on a silicon (Si) substrate. To maintain reasonable device dimensions, we have reduced trace and gap widths of the LCs to 4 \\upmu m. We increased the inductance from 16 to 60 \\upmu H to achieve a higher packing density, a requirement for FDM systems with large multiplexing factors. Additionally, the Si substrate yields low ESR values across the entire frequency range and lithography makes mass production of LC pairs possible. We reduced mutual inductance between inductors by placing them in a checkerboard pattern with the capacitors, thereby increasing physical distances between adjacent inductors. We also reduce magnetic coupling of inductors with external sources by evaporating a superconducting ground plane onto the backside of the substrate. We report on the development of lithographed LCs in the 1-5 MHz range for use with FDM systems. These resonators will be used by CMB polarization experiments such as Polarbear-2, Simons Array, and SPT-3G. Existing FDM systems have multiplexing factors up to 16× . We report the extension to 40× , i.e., Polarbear-2, and 68× , i.e., SPT-3G. We present the design criteria of Polarbear-2's LC circuits, the fabrication techniques, and the testing. Concerns such as yield, accuracy in frequency, loss, and mutual inductance between spatially neighboring channels will be discussed.

Generation of picosecond optical pulse based on chirp compensation

NASA Astrophysics Data System (ADS)

Sun, Xiaofeng; Yang, Jiaqian; Li, Shangyuan; Xue, Xiaoxiao; Zheng, Xiaoping; Zhou, Bingkun

2017-10-01

Picosecond optical pulses are widely used in optical communication systems, such as the optical time division multiplexing (OTDM) and photonic analog-to-digital converter (ADC). We have proposed and demonstrated a simple method to generate picosecond optical pulse using the mach-zehnder modulator (MZM), phase modulator (PM) and single model fiber (SMF). The phase modulator is used to generate a frequency chirp which varies periodically with time. The MZM is used to suppress the pedestal of the pulse and improve the performance of the pulse. The SMF is used to compensate the frequency chirp. We have carried out theoretical analysis and numerical simulation for the generation process of the picosecond optical pulse. The influence of phase shift between the modulation signals loaded on the MZM and PM is analyzed by numerical simulation and the conditions for the generation of picosecond optical pulse are given. The formula for calculating the optimum length of SMF which is used to compensate the linear chirp is given. The optical pulses with a repetition frequency of 10 GHz and a pulse width of 8.5 ps were obtained. The time-bandwidth product was as small as 1.09 and the timing jitter is as low as 83 fs.

A digital pixel cell for address event representation image convolution processing

NASA Astrophysics Data System (ADS)

Camunas-Mesa, Luis; Acosta-Jimenez, Antonio; Serrano-Gotarredona, Teresa; Linares-Barranco, Bernabe

2005-06-01

Address Event Representation (AER) is an emergent neuromorphic interchip communication protocol that allows for real-time virtual massive connectivity between huge number of neurons located on different chips. By exploiting high speed digital communication circuits (with nano-seconds timings), synaptic neural connections can be time multiplexed, while neural activity signals (with mili-seconds timings) are sampled at low frequencies. Also, neurons generate events according to their information levels. Neurons with more information (activity, derivative of activities, contrast, motion, edges,...) generate more events per unit time, and access the interchip communication channel more frequently, while neurons with low activity consume less communication bandwidth. AER technology has been used and reported for the implementation of various type of image sensors or retinae: luminance with local agc, contrast retinae, motion retinae,... Also, there has been a proposal for realizing programmable kernel image convolution chips. Such convolution chips would contain an array of pixels that perform weighted addition of events. Once a pixel has added sufficient event contributions to reach a fixed threshold, the pixel fires an event, which is then routed out of the chip for further processing. Such convolution chips have been proposed to be implemented using pulsed current mode mixed analog and digital circuit techniques. In this paper we present a fully digital pixel implementation to perform the weighted additions and fire the events. This way, for a given technology, there is a fully digital implementation reference against which compare the mixed signal implementations. We have designed, implemented and tested a fully digital AER convolution pixel. This pixel will be used to implement a full AER convolution chip for programmable kernel image convolution processing.

Demosaiced pixel super-resolution in digital holography for multiplexed computational color imaging on-a-chip (Conference Presentation)

NASA Astrophysics Data System (ADS)

Wu, Yichen; Zhang, Yibo; Luo, Wei; Ozcan, Aydogan

2017-03-01

Digital holographic on-chip microscopy achieves large space-bandwidth-products (e.g., >1 billion) by making use of pixel super-resolution techniques. To synthesize a digital holographic color image, one can take three sets of holograms representing the red (R), green (G) and blue (B) parts of the spectrum and digitally combine them to synthesize a color image. The data acquisition efficiency of this sequential illumination process can be improved by 3-fold using wavelength-multiplexed R, G and B illumination that simultaneously illuminates the sample, and using a Bayer color image sensor with known or calibrated transmission spectra to digitally demultiplex these three wavelength channels. This demultiplexing step is conventionally used with interpolation-based Bayer demosaicing methods. However, because the pixels of different color channels on a Bayer image sensor chip are not at the same physical location, conventional interpolation-based demosaicing process generates strong color artifacts, especially at rapidly oscillating hologram fringes, which become even more pronounced through digital wave propagation and phase retrieval processes. Here, we demonstrate that by merging the pixel super-resolution framework into the demultiplexing process, such color artifacts can be greatly suppressed. This novel technique, termed demosaiced pixel super-resolution (D-PSR) for digital holographic imaging, achieves very similar color imaging performance compared to conventional sequential R,G,B illumination, with 3-fold improvement in image acquisition time and data-efficiency. We successfully demonstrated the color imaging performance of this approach by imaging stained Pap smears. The D-PSR technique is broadly applicable to high-throughput, high-resolution digital holographic color microscopy techniques that can be used in resource-limited-settings and point-of-care offices.

Delay grid multiplexing: simple time-based multiplexing and readout method for silicon photomultipliers

NASA Astrophysics Data System (ADS)

Won, Jun Yeon; Ko, Guen Bae; Lee, Jae Sung

2016-10-01

In this paper, we propose a fully time-based multiplexing and readout method that uses the principle of the global positioning system. Time-based multiplexing allows simplifying the multiplexing circuits where the only innate traces that connect the signal pins of the silicon photomultiplier (SiPM) channels to the readout channels are used as the multiplexing circuit. Every SiPM channel is connected to the delay grid that consists of the traces on a printed circuit board, and the inherent transit times from each SiPM channel to the readout channels encode the position information uniquely. Thus, the position of each SiPM can be identified using the time difference of arrival (TDOA) measurements. The proposed multiplexing can also allow simplification of the readout circuit using the time-to-digital converter (TDC) implemented in a field-programmable gate array (FPGA), where the time-over-threshold (ToT) is used to extract the energy information after multiplexing. In order to verify the proposed multiplexing method, we built a positron emission tomography (PET) detector that consisted of an array of 4  ×  4 LGSO crystals, each with a dimension of 3  ×  3  ×  20 mm3, and one- to-one coupled SiPM channels. We first employed the waveform sampler as an initial study, and then replaced the waveform sampler with an FPGA-TDC to further simplify the readout circuits. The 16 crystals were clearly resolved using only the time information obtained from the four readout channels. The coincidence resolving times (CRTs) were 382 and 406 ps FWHM when using the waveform sampler and the FPGA-TDC, respectively. The proposed simple multiplexing and readout methods can be useful for time-of-flight (TOF) PET scanners.

Effect of soil temperature on optical frequency transfer through unidirectional dense-wavelength-division-multiplexing fiber-optic links.

PubMed

Pinkert, T J; Böll, O; Willmann, L; Jansen, G S M; Dijck, E A; Groeneveld, B G H M; Smets, R; Bosveld, F C; Ubachs, W; Jungmann, K; Eikema, K S E; Koelemeij, J C J

2015-02-01

Results of optical frequency transfer over a carrier-grade dense-wavelength-division-multiplexing (DWDM) optical fiber network are presented. The relation between soil temperature changes on a buried optical fiber and frequency changes of an optical carrier through the fiber is modeled. Soil temperatures, measured at various depths by the Royal Netherlands Meteorology Institute (KNMI) are compared with observed frequency variations through this model. A comparison of a nine-day record of optical frequency measurements through the 2×298  km fiber link with soil temperature data shows qualitative agreement. A soil temperature model is used to predict the link stability over longer periods (days-months-years). We show that optical frequency dissemination is sufficiently stable to distribute and compare, e.g., rubidium frequency standards over standard DWDM optical fiber networks using unidirectional fibers.

Fiber-Optic Propagation Effects in Long-Haul HF/VHF/UHF Analog Photonic Links

DTIC Science & Technology

2014-04-17

theoretical analysis of crosstalk in fiber optic wavelength division multiplexed systems is presented for the HF/VHF/UHF (1 MHz to 3 GHz) frequency...Street, Suite 1425 Arlington, VA 22203-1995 EW-271-003 6582 ONR Wavelength division multiplexing Crosstalk 05-03-2013 – 20-08-2014 TABLE OF CONTENTS...in optical fiber that can alter the phase relationship between signals in separate fibers or between signals that are multiplexed onto the same

Spectral multiplexing for scalable quantum photonics using an atomic frequency comb quantum memory and feed-forward control.

PubMed

Sinclair, Neil; Saglamyurek, Erhan; Mallahzadeh, Hassan; Slater, Joshua A; George, Mathew; Ricken, Raimund; Hedges, Morgan P; Oblak, Daniel; Simon, Christoph; Sohler, Wolfgang; Tittel, Wolfgang

2014-08-01

Future multiphoton applications of quantum optics and quantum information science require quantum memories that simultaneously store many photon states, each encoded into a different optical mode, and enable one to select the mapping between any input and a specific retrieved mode during storage. Here we show, with the example of a quantum repeater, how to employ spectrally multiplexed states and memories with fixed storage times that allow such mapping between spectral modes. Furthermore, using a Ti:Tm:LiNbO_{3} waveguide cooled to 3 K, a phase modulator, and a spectral filter, we demonstrate storage followed by the required feed-forward-controlled frequency manipulation with time-bin qubits encoded into up to 26 multiplexed spectral modes and 97% fidelity.

Evaluation of selective control information detection scheme in orthogonal frequency division multiplexing-based radio-over-fiber and visible light communication links

NASA Astrophysics Data System (ADS)

Dalarmelina, Carlos A.; Adegbite, Saheed A.; Pereira, Esequiel da V.; Nunes, Reginaldo B.; Rocha, Helder R. O.; Segatto, Marcelo E. V.; Silva, Jair A. L.

2017-05-01

Block-level detection is required to decode what may be classified as selective control information (SCI) such as control format indicator in 4G-long-term evolution systems. Using optical orthogonal frequency division multiplexing over radio-over-fiber (RoF) links, we report the experimental evaluation of an SCI detection scheme based on a time-domain correlation (TDC) technique in comparison with the conventional maximum likelihood (ML) approach. When compared with the ML method, it is shown that the TDC method improves detection performance over both 20 and 40 km of standard single mode fiber (SSMF) links. We also report a performance analysis of the TDC scheme in noisy visible light communication channel models after propagation through 40 km of SSMF. Experimental and simulation results confirm that the TDC method is attractive for practical orthogonal frequency division multiplexing-based RoF and fiber-wireless systems. Unlike the ML method, another key benefit of the TDC is that it requires no channel estimation.

Performance Evaluation of High Speed Multicarrier System for Optical Wireless Communication

NASA Astrophysics Data System (ADS)

Mathur, Harshita; Deepa, T.; Bartalwar, Sophiya

2018-04-01

Optical wireless communication (OWC) in the infrared and visible range is quite impressive solution, especially where radio communication face challenges. Visible light communication (VLC) uses visible light over a range of 400 and 800 THz and is a subdivision of OWC technologies. With an increasing demand for use of wireless communications, wireless access via Wi-Fi is facing many challenges especially in terms of capacity, availability, security and efficiency. VLC uses intensity modulation and direct detection (IM/DD) techniques and hence they require the signals to certainly be real valued positive sequences. These constraints pose limitation on digital modulation techniques. These limitations result in spectrum-efficiency or power-efficiency losses. In this paper, we investigate an amplitude shift keying (ASK) based orthogonal frequency division multiplexing (OFDM) signal transmission scheme using LabVIEW for VLC technology.

The 10 Hottest Technologies in Telecom.

ERIC Educational Resources Information Center

Flanagan, Patrick

1997-01-01

Presents the fourth annual listing of the 10 "hottest" telecommunications technologies. Describes Web broadcasting, remote-access servers, extranets, Internet telephony, enterprise network directory services, Web site management tools, IP (Internet Protocols) switching, wavelength division multiplexing, digital subscriber lines, and…

Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells.

PubMed

Ullal, Adeeti V; Weissleder, Ralph

2015-01-01

We describe a DNA-barcoded antibody sensing technique for single cell protein analysis in which the barcodes are photocleaved and digitally detected without amplification steps (Ullal et al., Sci Transl Med 6:219, 2014). After photocleaving the unique ~70 mer DNA barcodes we use a fluorescent hybridization technology for detection, similar to what is commonly done for nucleic acid readouts. This protocol offers a simple method for multiplexed protein detection using 100+ antibodies and can be performed on clinical samples as well as single cells.

Self-balanced modulation and magnetic rebalancing method for parallel multilevel inverters

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Hui; Shi, Yanjun

A self-balanced modulation method and a closed-loop magnetic flux rebalancing control method for parallel multilevel inverters. The combination of the two methods provides for balancing of the magnetic flux of the inter-cell transformers (ICTs) of the parallel multilevel inverters without deteriorating the quality of the output voltage. In various embodiments a parallel multi-level inverter modulator is provide including a multi-channel comparator to generate a multiplexed digitized ideal waveform for a parallel multi-level inverter and a finite state machine (FSM) module coupled to the parallel multi-channel comparator, the FSM module to receive the multiplexed digitized ideal waveform and to generate amore » pulse width modulated gate-drive signal for each switching device of the parallel multi-level inverter. The system and method provides for optimization of the output voltage spectrum without influence the magnetic balancing.« less

Memory-based frame synchronizer. [for digital communication systems

NASA Technical Reports Server (NTRS)

Stattel, R. J.; Niswander, J. K. (Inventor)

1981-01-01

A frame synchronizer for use in digital communications systems wherein data formats can be easily and dynamically changed is described. The use of memory array elements provide increased flexibility in format selection and sync word selection in addition to real time reconfiguration ability. The frame synchronizer comprises a serial-to-parallel converter which converts a serial input data stream to a constantly changing parallel data output. This parallel data output is supplied to programmable sync word recognizers each consisting of a multiplexer and a random access memory (RAM). The multiplexer is connected to both the parallel data output and an address bus which may be connected to a microprocessor or computer for purposes of programming the sync word recognizer. The RAM is used as an associative memory or decorder and is programmed to identify a specific sync word. Additional programmable RAMs are used as counter decoders to define word bit length, frame word length, and paragraph frame length.

System for Processing Coded OFDM Under Doppler and Fading

NASA Technical Reports Server (NTRS)

Tsou, Haiping; Darden, Scott; Lee, Dennis; Yan, Tsun-Yee

2005-01-01

An advanced communication system has been proposed for transmitting and receiving coded digital data conveyed as a form of quadrature amplitude modulation (QAM) on orthogonal frequency-division multiplexing (OFDM) signals in the presence of such adverse propagation-channel effects as large dynamic Doppler shifts and frequency-selective multipath fading. Such adverse channel effects are typical of data communications between mobile units or between mobile and stationary units (e.g., telemetric transmissions from aircraft to ground stations). The proposed system incorporates novel signal processing techniques intended to reduce the losses associated with adverse channel effects while maintaining compatibility with the high-speed physical layer specifications defined for wireless local area networks (LANs) as the standard 802.11a of the Institute of Electrical and Electronics Engineers (IEEE 802.11a). OFDM is a multi-carrier modulation technique that is widely used for wireless transmission of data in LANs and in metropolitan area networks (MANs). OFDM has been adopted in IEEE 802.11a and some other industry standards because it affords robust performance under frequency-selective fading. However, its intrinsic frequency-diversity feature is highly sensitive to synchronization errors; this sensitivity poses a challenge to preserve coherence between the component subcarriers of an OFDM system in order to avoid intercarrier interference in the presence of large dynamic Doppler shifts as well as frequency-selective fading. As a result, heretofore, the use of OFDM has been limited primarily to applications involving small or zero Doppler shifts. The proposed system includes a digital coherent OFDM communication system that would utilize enhanced 802.1la-compatible signal-processing algorithms to overcome effects of frequency-selective fading and large dynamic Doppler shifts. The overall transceiver design would implement a two-frequency-channel architecture (see figure) that would afford frequency diversity for reducing the adverse effects of multipath fading. By using parallel concatenated convolutional codes (also known as Turbo codes) across the dual-channel and advanced OFDM signal processing within each channel, the proposed system is intended to achieve at least an order of magnitude improvement in received signal-to-noise ratio under adverse channel effects while preserving spectral efficiency.

Design of elliptical-core mode-selective photonic lanterns with six modes for MIMO-free mode division multiplexing systems.

PubMed

Sai, Xiaowei; Li, Yan; Yang, Chen; Li, Wei; Qiu, Jifang; Hong, Xiaobin; Zuo, Yong; Guo, Hongxiang; Tong, Weijun; Wu, Jian

2017-11-01

Elliptical-core few mode fiber (EC-FMF) is used in a mode division multiplexing (MDM) transmission system to release multiple-input-multiple-output (MIMO) digital-signal-processing, which reduces the cost and the complexity of the receiver. However, EC-FMF does not match with conventional multiplexers/de-multiplexers (MUXs/DeMUXs) such as a photonic lantern, leading to extra mode coupling loss and crosstalk. We design elliptical-core mode-selective photonic lanterns (EC-MSPLs) with six modes, which can match well with EC-FMF in MIMO-free MDM systems. Simulation of the EC-MSPL using the beam propagation method was demonstrated employing a combination of either step-index or graded-index fibers with six different sizes of cores, and the taper transition length of 8 cm or 4 cm. Through numerical simulations and optimizations, both types of photonic lanterns can realize low loss transmission and low crosstalk of below -20.0  dB for all modes.

Scalable modulation technology and the tradeoff of reach, spectral efficiency, and complexity

NASA Astrophysics Data System (ADS)

Bosco, Gabriella; Pilori, Dario; Poggiolini, Pierluigi; Carena, Andrea; Guiomar, Fernando

2017-01-01

Bandwidth and capacity demand in metro, regional, and long-haul networks is increasing at several tens of percent per year, driven by video streaming, cloud computing, social media and mobile applications. To sustain this traffic growth, an upgrade of the widely deployed 100-Gbit/s long-haul optical systems, based on polarization multiplexed quadrature phase-shift keying (PM-QPSK) modulation format associated with coherent detection and digital signal processing (DSP), is mandatory. In fact, optical transport techniques enabling a per-channel bit rate beyond 100 Gbit/s have recently been the object of intensive R and D activities, aimed at both improving the spectral efficiency and lowering the cost per bit in fiber transmission systems. In this invited contribution, we review the different available options to scale the per-channel bit-rate to 400 Gbit/s and beyond, i.e. symbol-rate increase, use of higher-order quadrature amplitude modulation (QAM) modulation formats and use of super-channels with DSP-enabled spectral shaping and advanced multiplexing technologies. In this analysis, trade-offs of system reach, spectral efficiency and transceiver complexity are addressed. Besides scalability, next generation optical networks will require a high degree of flexibility in the transponders, which should be able to dynamically adapt the transmission rate and bandwidth occupancy to the light path characteristics. In order to increase the flexibility of these transponders (often referred to as "flexponders"), several advanced modulation techniques have recently been proposed, among which sub-carrier multiplexing, hybrid formats (over time, frequency and polarization), and constellation shaping. We review these techniques, highlighting their limits and potential in terms of performance, complexity and flexibility.

Low band gap frequencies and multiplexing properties in 1D and 2D mass spring structures

NASA Astrophysics Data System (ADS)

Aly, Arafa H.; Mehaney, Ahmed

2016-11-01

This study reports on the propagation of elastic waves in 1D and 2D mass spring structures. An analytical and computation model is presented for the 1D and 2D mass spring systems with different examples. An enhancement in the band gap values was obtained by modeling the structures to obtain low frequency band gaps at small dimensions. Additionally, the evolution of the band gap as a function of mass value is discussed. Special attention is devoted to the local resonance property in frequency ranges within the gaps in the band structure for the corresponding infinite periodic lattice in the 1D and 2D mass spring system. A linear defect formed of a row of specific masses produces an elastic waveguide that transmits at the narrow pass band frequency. The frequency of the waveguides can be selected by adjusting the mass and stiffness coefficients of the materials constituting the waveguide. Moreover, we pay more attention to analyze the wave multiplexer and DE-multiplexer in the 2D mass spring system. We show that two of these tunable waveguides with alternating materials can be employed to filter and separate specific frequencies from a broad band input signal. The presented simulation data is validated through comparison with the published research, and can be extended in the development of resonators and MEMS verification.

Derivation of GFDM Based on OFDM Principles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hussein Moradi; Behrouz Farhang-Boroujeny

2015-06-01

This paper starts with discussing the principle based on which the celebrated orthogonal frequency division multiplexing (OFDM) signals are constructed. It then extends the same principle to construct the newly introduced generalized frequency division multiplexing (GFDM) signals. This novel derivation sheds light on some interesting properties of GFDM. In particular, our derivation seamlessly leads to an implementation of GFDM transmitter which has significantly lower complexity than what has been reported so far. Our derivation also facilitates a trivial understanding of how GFDM (similar to OFDM) can be applied in MIMO channels.

Development and inter-laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines.

PubMed

Košir, Alexandra Bogožalec; Spilsberg, Bjørn; Holst-Jensen, Arne; Žel, Jana; Dobnik, David

2017-08-17

Quantification of genetically modified organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds. Standard-curve-based simplex quantitative polymerase chain reaction (qPCR) is the prevailing technology, which is often combined with screening analysis. With the rapidly growing number of GMOs on the world market, qPCR analysis becomes laborious and expensive. Innovative cost-effective approaches are therefore urgently needed. Here, we report the development and inter-laboratory assessment of multiplex assays to quantify GMO soybean using droplet digital PCR (ddPCR). The assays were developed to facilitate testing of foods and feed for compliance with current GMO regulations in the European Union (EU). Within the EU, the threshold for labelling is 0.9% for authorised GMOs per ingredient. Furthermore, the EU has set a technical zero tolerance limit of 0.1% for certain unauthorised GMOs. The novel multiplex ddPCR assays developed target 11 GMO soybean lines that are currently authorised, and four that are tolerated, pending authorisation in the EU. Potential significant improvements in cost efficiency are demonstrated. Performance was assessed for the critical parameters, including limits of detection and quantification, and trueness, repeatability, and robustness. Inter-laboratory performance was also determined on a number of proficiency programme and real-life samples.

Digital Transplantation Pathology: Combining Whole Slide Imaging, Multiplex Staining, and Automated Image Analysis

PubMed Central

Isse, Kumiko; Lesniak, Andrew; Grama, Kedar; Roysam, Badrinath; Minervini, Martha I.; Demetris, Anthony J

2013-01-01

Conventional histopathology is the gold standard for allograft monitoring, but its value proposition is increasingly questioned. “-Omics” analysis of tissues, peripheral blood and fluids and targeted serologic studies provide mechanistic insights into allograft injury not currently provided by conventional histology. Microscopic biopsy analysis, however, provides valuable and unique information: a) spatial-temporal relationships; b) rare events/cells; c) complex structural context; and d) integration into a “systems” model. Nevertheless, except for immunostaining, no transformative advancements have “modernized” routine microscopy in over 100 years. Pathologists now team with hardware and software engineers to exploit remarkable developments in digital imaging, nanoparticle multiplex staining, and computational image analysis software to bridge the traditional histology - global “–omic” analyses gap. Included are side-by-side comparisons, objective biopsy finding quantification, multiplexing, automated image analysis, and electronic data and resource sharing. Current utilization for teaching, quality assurance, conferencing, consultations, research and clinical trials is evolving toward implementation for low-volume, high-complexity clinical services like transplantation pathology. Cost, complexities of implementation, fluid/evolving standards, and unsettled medical/legal and regulatory issues remain as challenges. Regardless, challenges will be overcome and these technologies will enable transplant pathologists to increase information extraction from tissue specimens and contribute to cross-platform biomarker discovery for improved outcomes. PMID:22053785

Time-varying multiplex network: Intralayer and interlayer synchronization

NASA Astrophysics Data System (ADS)

Rakshit, Sarbendu; Majhi, Soumen; Bera, Bidesh K.; Sinha, Sudeshna; Ghosh, Dibakar

2017-12-01

A large class of engineered and natural systems, ranging from transportation networks to neuronal networks, are best represented by multiplex network architectures, namely a network composed of two or more different layers where the mutual interaction in each layer may differ from other layers. Here we consider a multiplex network where the intralayer coupling interactions are switched stochastically with a characteristic frequency. We explore the intralayer and interlayer synchronization of such a time-varying multiplex network. We find that the analytically derived necessary condition for intralayer and interlayer synchronization, obtained by the master stability function approach, is in excellent agreement with our numerical results. Interestingly, we clearly find that the higher frequency of switching links in the layers enhances both intralayer and interlayer synchrony, yielding larger windows of synchronization. Further, we quantify the resilience of synchronous states against random perturbations, using a global stability measure based on the concept of basin stability, and this reveals that intralayer coupling strength is most crucial for determining both intralayer and interlayer synchrony. Lastly, we investigate the robustness of interlayer synchronization against a progressive demultiplexing of the multiplex structure, and we find that for rapid switching of intralayer links, the interlayer synchronization persists even when a large number of interlayer nodes are disconnected.

Time-varying multiplex network: Intralayer and interlayer synchronization.

PubMed

Rakshit, Sarbendu; Majhi, Soumen; Bera, Bidesh K; Sinha, Sudeshna; Ghosh, Dibakar

2017-12-01

A large class of engineered and natural systems, ranging from transportation networks to neuronal networks, are best represented by multiplex network architectures, namely a network composed of two or more different layers where the mutual interaction in each layer may differ from other layers. Here we consider a multiplex network where the intralayer coupling interactions are switched stochastically with a characteristic frequency. We explore the intralayer and interlayer synchronization of such a time-varying multiplex network. We find that the analytically derived necessary condition for intralayer and interlayer synchronization, obtained by the master stability function approach, is in excellent agreement with our numerical results. Interestingly, we clearly find that the higher frequency of switching links in the layers enhances both intralayer and interlayer synchrony, yielding larger windows of synchronization. Further, we quantify the resilience of synchronous states against random perturbations, using a global stability measure based on the concept of basin stability, and this reveals that intralayer coupling strength is most crucial for determining both intralayer and interlayer synchrony. Lastly, we investigate the robustness of interlayer synchronization against a progressive demultiplexing of the multiplex structure, and we find that for rapid switching of intralayer links, the interlayer synchronization persists even when a large number of interlayer nodes are disconnected.

Shared protection based virtual network mapping in space division multiplexing optical networks

NASA Astrophysics Data System (ADS)

Zhang, Huibin; Wang, Wei; Zhao, Yongli; Zhang, Jie

2018-05-01

Space Division Multiplexing (SDM) has been introduced to improve the capacity of optical networks. In SDM optical networks, there are multiple cores/modes in each fiber link, and spectrum resources are multiplexed in both frequency and core/modes dimensions. Enabled by network virtualization technology, one SDM optical network substrate can be shared by several virtual networks operators. Similar with point-to-point connection services, virtual networks (VN) also need certain survivability to guard against network failures. Based on customers' heterogeneous requirements on the survivability of their virtual networks, this paper studies the shared protection based VN mapping problem and proposes a Minimum Free Frequency Slots (MFFS) mapping algorithm to improve spectrum efficiency. Simulation results show that the proposed algorithm can optimize SDM optical networks significantly in terms of blocking probability and spectrum utilization.

Simple, Sensitive and Accurate Multiplex Detection of Clinically Important Melanoma DNA Mutations in Circulating Tumour DNA with SERS Nanotags

PubMed Central

Wee, Eugene J.H.; Wang, Yuling; Tsao, Simon Chang-Hao; Trau, Matt

2016-01-01

Sensitive and accurate identification of specific DNA mutations can influence clinical decisions. However accurate diagnosis from limiting samples such as circulating tumour DNA (ctDNA) is challenging. Current approaches based on fluorescence such as quantitative PCR (qPCR) and more recently, droplet digital PCR (ddPCR) have limitations in multiplex detection, sensitivity and the need for expensive specialized equipment. Herein we describe an assay capitalizing on the multiplexing and sensitivity benefits of surface-enhanced Raman spectroscopy (SERS) with the simplicity of standard PCR to address the limitations of current approaches. This proof-of-concept method could reproducibly detect as few as 0.1% (10 copies, CV < 9%) of target sequences thus demonstrating the high sensitivity of the method. The method was then applied to specifically detect three important melanoma mutations in multiplex. Finally, the PCR/SERS assay was used to genotype cell lines and ctDNA from serum samples where results subsequently validated with ddPCR. With ddPCR-like sensitivity and accuracy yet at the convenience of standard PCR, we believe this multiplex PCR/SERS method could find wide applications in both diagnostics and research. PMID:27446486

Simple, Sensitive and Accurate Multiplex Detection of Clinically Important Melanoma DNA Mutations in Circulating Tumour DNA with SERS Nanotags.

PubMed

Wee, Eugene J H; Wang, Yuling; Tsao, Simon Chang-Hao; Trau, Matt

2016-01-01

Sensitive and accurate identification of specific DNA mutations can influence clinical decisions. However accurate diagnosis from limiting samples such as circulating tumour DNA (ctDNA) is challenging. Current approaches based on fluorescence such as quantitative PCR (qPCR) and more recently, droplet digital PCR (ddPCR) have limitations in multiplex detection, sensitivity and the need for expensive specialized equipment. Herein we describe an assay capitalizing on the multiplexing and sensitivity benefits of surface-enhanced Raman spectroscopy (SERS) with the simplicity of standard PCR to address the limitations of current approaches. This proof-of-concept method could reproducibly detect as few as 0.1% (10 copies, CV < 9%) of target sequences thus demonstrating the high sensitivity of the method. The method was then applied to specifically detect three important melanoma mutations in multiplex. Finally, the PCR/SERS assay was used to genotype cell lines and ctDNA from serum samples where results subsequently validated with ddPCR. With ddPCR-like sensitivity and accuracy yet at the convenience of standard PCR, we believe this multiplex PCR/SERS method could find wide applications in both diagnostics and research.

Simultaneous frequency stabilization and high-power dense wavelength division multiplexing (HP-DWDM) using an external cavity based on volume Bragg gratings (VBGs)

NASA Astrophysics Data System (ADS)

Hengesbach, Stefan; Klein, Sarah; Holly, Carlo; Witte, Ulrich; Traub, Martin; Hoffmann, Dieter

2016-03-01

Multiplexing technologies enable the development of high-brightness diode lasers for direct industrial applications. We present a High-Power Dense Wavelength Division Multiplexer (HP-DWDM) with an average channel spacing of 1.7 (1.5) nm and a subsequent external cavity mirror to provide feedback for frequency stabilization and multiplexing in one step. The "self-optimizing" multiplexing unit consists of four reflective Volume Bragg Gratings (VBGs) with 99% diffraction efficiency and seven dielectric mirrors to overlay the radiation of five input channels with an adjustable channel spacing of 1-2 nm. In detail, we focus on the analysis of the overall optical efficiency, the change of the beam parameter product and the spectral width. The performance is demonstrated using five 90 μm multimode 9xx single emitters with M2<=17. Because of the feedback the lateral (multimodal) spatial and angular intensity distribution changes strongly and the beam parameter product decreases by a factor of 1.2 to 1.9. Thereby the angular intensity distribution is more affected than the width of the beam waist. The spectral width per emitter decreases to 3-200 pm (FWHM) depending on the injection current and the reflectance of the feedback mirror (0.75%, 1.5%, 4%, 6% or 8%). The overall optical multiplexing efficiency ranges between 77% and 86%. With some modifications (e.g. enhanced AR-coatings) we expect 90-95%.

Simultaneous measurements of multiple parameters at elevated temperature using a frequency-division multiplexing scheme with tunable diode lasers.

PubMed

Cai, Tingdong; Gao, Guangzhen; Liu, Ying

2012-10-01

A multiplexed diode-laser sensor system based on second harmonic detection of wavelength modulation spectroscopy (WMS) is developed for application at elevated temperatures with two near-infrared diode lasers multiplexed using a frequency-division multiplexing scheme. One laser is tuned over a H(2)O line pair near 7079.176 and 7079.855 cm(-1), and another laser is tuned over a pair of CO(2) and CO lines near 6361.250 and 6361.344 cm(-1). Temperature and concentrations of H(2)O, CO(2), and CO could be measured simultaneously by this system. In order to remove the need for calibration and correct for transmission variation due to beam steering, mechanical misalignments, soot, and windows fouling, the WMS-1f normalized 2f method is used. Demonstration experiments are conducted in a heated static cell. The precision of temperature and the concentrations for H(2)O, CO(2), and CO are found to be 1.57%, 3.87%, 3.01%, and 3.58%, respectively. These results illustrate the potential of this sensor for applications at high temperatures.

Hybrid time-frequency domain equalization based on sign-sign joint decision multimodulus algorithm for 6 × 6 mode division multiplexing system

NASA Astrophysics Data System (ADS)

Li, Jiao; Hu, Guijun; Gong, Caili; Li, Li

2018-02-01

In this paper, we propose a hybrid time-frequency domain sign-sign joint decision multimodulus algorithm (Hybrid-SJDMMA) for mode-demultiplexing in a 6 × 6 mode division multiplexing (MDM) system with high-order QAM modulation. The equalization performance of Hybrid-SJDMMA was evaluated and compared with the frequency domain multimodulus algorithm (FD-MMA) and the hybrid time-frequency domain sign-sign multimodulus algorithm (Hybrid-SMMA). Simulation results revealed that Hybrid-SJDMMA exhibits a significantly lower computational complexity than FD-MMA, and its convergence speed is similar to that of FD-MMA. Additionally, the bit-error-rate performance of Hybrid-SJDMMA was obviously better than FD-MMA and Hybrid-SMMA for 16 QAM and 64 QAM.

Fiber-wireless transmission system of 108  Gb/sdata over 80 km fiber and 2×2multiple-input multiple-output wireless links at 100 GHz W-band frequency.

PubMed

Li, Xinying; Dong, Ze; Yu, Jianjun; Chi, Nan; Shao, Yufeng; Chang, G K

2012-12-15

We experimentally demonstrate a seamlessly integrated fiber-wireless system that delivers a 108  Gb/s signal through 80 km fiber and 1 m wireless transport over free space at 100 GHz adopting polarization-division-multiplexing quadrature-phase-shift-keying (PDM-QPSK) modulation and heterodyning coherent detection. The X- and Y-polarization components of the optical PDM-QPSK baseband signal are simultaneously upconverted to 100 GHz wireless carrier by optical polarization-diversity heterodyne beating, and then independently transmitted and received by two pairs of transmitter and receiver antennas, which form a 2×2 multiple-input multiple-output wireless link. At the wireless receiver, two-stage downconversion is performed firstly in the analog domain based on balanced mixer and sinusoidal radio frequency signal, and then in the digital domain based on digital signal processing (DSP). Polarization demultiplexing is realized by the constant modulus algorithm in the DSP part at the receiver. The bit-error ratio for the 108  Gb/s PDM-QPSK signal is less than the pre-forward-error-correction threshold of 3.8×10(-3) after both 1 m wireless delivery at 100 GHz and 80 km single-mode fiber-28 transmission. To our knowledge, this is the first demonstration to realize 100  Gb/s signal delivery through both fiber and wireless links at 100 GHz.

Networks: A Review of Their Technology, Architecture, and Implementation.

ERIC Educational Resources Information Center

Learn, Larry L.

1988-01-01

This overview of network-related technologies covers network elements, analog and digital signals, transmission media and their characteristics, equipment certification, multiplexing, network types, access technologies, network architectures local-area network technologies and attributes, protocols, internetworking, fiber optics versus satellites,…

Design and application of pulse information acquisition and analysis system with dynamic recognition in traditional Chinese medicine.

PubMed

Zhang, Jian; Niu, Xin; Yang, Xue-zhi; Zhu, Qing-wen; Li, Hai-yan; Wang, Xuan; Zhang, Zhi-guo; Sha, Hong

2014-09-01

To design the pulse information which includes the parameter of pulse-position, pulse-number, pulse-shape and pulse-force acquisition and analysis system with function of dynamic recognition, and research the digitalization and visualization of some common cardiovascular mechanism of single pulse. To use some flexible sensors to catch the radial artery pressure pulse wave and utilize the high frequency B mode ultrasound scanning technology to synchronously obtain the information of radial extension and axial movement, by the way of dynamic images, then the gathered information was analyzed and processed together with ECG. Finally, the pulse information acquisition and analysis system was established which has the features of visualization and dynamic recognition, and it was applied to serve for ten healthy adults. The new system overcome the disadvantage of one-dimensional pulse information acquisition and process method which was common used in current research area of pulse diagnosis in traditional Chinese Medicine, initiated a new way of pulse diagnosis which has the new features of dynamic recognition, two-dimensional information acquisition, multiplex signals combination and deep data mining. The newly developed system could translate the pulse signals into digital, visual and measurable motion information of vessel.

Spectrally efficient digitized radio-over-fiber system with k-means clustering-based multidimensional quantization.

PubMed

Zhang, Lu; Pang, Xiaodan; Ozolins, Oskars; Udalcovs, Aleksejs; Popov, Sergei; Xiao, Shilin; Hu, Weisheng; Chen, Jiajia

2018-04-01

We propose a spectrally efficient digitized radio-over-fiber (D-RoF) system by grouping highly correlated neighboring samples of the analog signals into multidimensional vectors, where the k-means clustering algorithm is adopted for adaptive quantization. A 30  Gbit/s D-RoF system is experimentally demonstrated to validate the proposed scheme, reporting a carrier aggregation of up to 40 100 MHz orthogonal frequency division multiplexing (OFDM) channels with quadrate amplitude modulation (QAM) order of 4 and an aggregation of 10 100 MHz OFDM channels with a QAM order of 16384. The equivalent common public radio interface rates from 37 to 150  Gbit/s are supported. Besides, the error vector magnitude (EVM) of 8% is achieved with the number of quantization bits of 4, and the EVM can be further reduced to 1% by increasing the number of quantization bits to 7. Compared with conventional pulse coding modulation-based D-RoF systems, the proposed D-RoF system improves the signal-to-noise-ratio up to ∼9  dB and greatly reduces the EVM, given the same number of quantization bits.

Fading-free transmission of 124-Gb/s PDM-DMT signal over 100-km SSMF using digital carrier regeneration.

PubMed

Li, Cai; Hu, Rong; Yang, Qi; Luo, Ming; Li, Wei; Yu, Shaohua

2016-01-25

The coherent reception of intensity modulated signal has been recently widely investigated, in which the signal is recovered by the envelop detection. High linewidth tolerance is achieved with such scheme. However, strong optical carrier exists during the transmission, which degrades the optical power efficiency. In this paper, an efficient modulation scheme for discrete multi-tone (DMT) signal is proposed based on the Mach-Zehnder modulator (MZM). Different from the traditional intensity modulation, the proposed method employs both intensity and phase domain. Thus, the optical carrier power can be greatly reduced by adjusting the bias of MZM around the null point. By employing coherent detection and digital carrier regeneration (DCR), the carrier suppressed DMT signal can be recovered using envelop detection. No carrier frequency or phase estimation is required. Numerical investigations are made to demonstrate the feasibility, in which significant improvements are found for the proposed DCR method, showing great tolerance against laser linewidth and carrier power reduction. Finally, a 124-Gb/s transmission of polarization-division multiplexed DMT (PDM-DMT) signal is demonstrated over 100-km SSMF, with only -8 dB optical carrier to signal power ratio (CSPR).

An Efficient, Highly Flexible Multi-Channel Digital Downconverter Architecture

NASA Technical Reports Server (NTRS)

Goodhart, Charles E.; Soriano, Melissa A.; Navarro, Robert; Trinh, Joseph T.; Sigman, Elliott H.

2013-01-01

In this innovation, a digital downconverter has been created that produces a large (16 or greater) number of output channels of smaller bandwidths. Additionally, this design has the flexibility to tune each channel independently to anywhere in the input bandwidth to cover a wide range of output bandwidths (from 32 MHz down to 1 kHz). Both the flexibility in channel frequency selection and the more than four orders of magnitude range in output bandwidths (decimation rates from 32 to 640,000) presented significant challenges to be solved. The solution involved breaking the digital downconversion process into a two-stage process. The first stage is a 2 oversampled filter bank that divides the whole input bandwidth as a real input signal into seven overlapping, contiguous channels represented with complex samples. Using the symmetry of the sine and cosine functions in a similar way to that of an FFT (fast Fourier transform), this downconversion is very efficient and gives seven channels fixed in frequency. An arbitrary number of smaller bandwidth channels can be formed from second-stage downconverters placed after the first stage of downconversion. Because of the overlapping of the first stage, there is no gap in coverage of the entire input bandwidth. The input to any of the second-stage downconverting channels has a multiplexer that chooses one of the seven wideband channels from the first stage. These second-stage downconverters take up fewer resources because they operate at lower bandwidths than doing the entire downconversion process from the input bandwidth for each independent channel. These second-stage downconverters are each independent with fine frequency control tuning, providing extreme flexibility in positioning the center frequency of a downconverted channel. Finally, these second-stage downconverters have flexible decimation factors over four orders of magnitude The algorithm was developed to run in an FPGA (field programmable gate array) at input data sampling rates of up to 1,280 MHz. The current implementation takes a 1,280-MHz real input, and first breaks it up into seven 160-MHz complex channels, each spaced 80 MHz apart. The eighth channel at baseband was not required for this implementation, and led to more optimization. Afterwards, 16 second stage narrow band channels with independently tunable center frequencies and bandwidth settings are implemented A future implementation in a larger Xilinx FPGA will hold up to 32 independent second-stage channels.

Molecular Analysis-Based Genetic Characterization of a Cohort of Patients with Duchenne and Becker Muscular Dystrophy in Eastern China.

PubMed

Zhao, Hui-Hui; Sun, Xue-Ping; Shi, Ming-Chao; Yi, Yong-Xiang; Cheng, Hong; Wang, Xing-Xia; Xu, Qing-Cheng; Ma, Hong-Ming; Wu, Hao-Quan; Jin, Qing-Wen; Niu, Qi

2018-04-05

Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are common X-linked recessive neuromuscular disorders caused by mutations in dystrophin gene. Multiplex polymerase chain reaction (multiplex PCR) and multiplex ligation-dependent probe amplification (MLPA) are the most common methods for detecting dystrophin gene mutations. This study aimed to contrast the two methods and discern the genetic characterization of patients with DMD/BMD in Eastern China. We collected 121 probands, 64 mothers of probands, and 15 fetuses in our study. The dystrophin gene was detected by multiplex PCR primarily in 28 probands, and MLPA was used in multiplex PCR-negative cases subsequently. The dystrophin gene of the remaining 93 probands and 62 female potential carriers was tested by MLPA directly. In fetuses, multiplex PCR and MLPA were performed on 4 fetuses and 10 fetuses, respectively. In addition, sequencing was also performed in 4 probands with negative MLPA. We found that 61.98% of the subjects had genetic mutations including deletions (50.41%) and duplications (11.57%). There were 43.75% of mothers as carriers of the mutation. In 15 fetuses, 2 out of 7 male fetuses were found to be unhealthy and 2 out of 8 female fetuses were found to be carriers. Exons 3-26 and 45-52 have the maximum frequency in mutation regions. In the frequency of exons individually, exon 47 and exon 50 were the most common in deleted regions and exons 5, 6, and 7 were found most frequently in duplicated regions. MLPA has better productivity and sensitivity than multiplex PCR. Prenatal diagnosis should be applied in DMD high-risk fetuses to reduce the disease incidence. Furthermore, it is the responsibility of physicians to inform female carriers the importance of prenatal diagnosis.

A time and frequency synchronization method for CO-OFDM based on CMA equalizers

NASA Astrophysics Data System (ADS)

Ren, Kaixuan; Li, Xiang; Huang, Tianye; Cheng, Zhuo; Chen, Bingwei; Wu, Xu; Fu, Songnian; Ping, Perry Shum

2018-06-01

In this paper, an efficient time and frequency synchronization method based on a new training symbol structure is proposed for polarization division multiplexing (PDM) coherent optical orthogonal frequency division multiplexing (CO-OFDM) systems. The coarse timing synchronization is achieved by exploiting the correlation property of the first training symbol, and the fine timing synchronization is accomplished by using the time-domain symmetric conjugate of the second training symbol. Furthermore, based on these training symbols, a constant modulus algorithm (CMA) is proposed for carrier frequency offset (CFO) estimation. Theoretical analysis and simulation results indicate that the algorithm has the advantages of robustness to poor optical signal-to-noise ratio (OSNR) and chromatic dispersion (CD). The frequency offset estimation range can achieve [ -Nsc/2 ΔfN , + Nsc/2 ΔfN ] GHz with the mean normalized estimation error below 12 × 10-3 even under the condition of OSNR as low as 10 dB.

Microresonator-based solitons for massively parallel coherent optical communications

NASA Astrophysics Data System (ADS)

Marin-Palomo, Pablo; Kemal, Juned N.; Karpov, Maxim; Kordts, Arne; Pfeifle, Joerg; Pfeiffer, Martin H. P.; Trocha, Philipp; Wolf, Stefan; Brasch, Victor; Anderson, Miles H.; Rosenberger, Ralf; Vijayan, Kovendhan; Freude, Wolfgang; Kippenberg, Tobias J.; Koos, Christian

2017-06-01

Solitons are waveforms that preserve their shape while propagating, as a result of a balance of dispersion and nonlinearity. Soliton-based data transmission schemes were investigated in the 1980s and showed promise as a way of overcoming the limitations imposed by dispersion of optical fibres. However, these approaches were later abandoned in favour of wavelength-division multiplexing schemes, which are easier to implement and offer improved scalability to higher data rates. Here we show that solitons could make a comeback in optical communications, not as a competitor but as a key element of massively parallel wavelength-division multiplexing. Instead of encoding data on the soliton pulse train itself, we use continuous-wave tones of the associated frequency comb as carriers for communication. Dissipative Kerr solitons (DKSs) (solitons that rely on a double balance of parametric gain and cavity loss, as well as dispersion and nonlinearity) are generated as continuously circulating pulses in an integrated silicon nitride microresonator via four-photon interactions mediated by the Kerr nonlinearity, leading to low-noise, spectrally smooth, broadband optical frequency combs. We use two interleaved DKS frequency combs to transmit a data stream of more than 50 terabits per second on 179 individual optical carriers that span the entire telecommunication C and L bands (centred around infrared telecommunication wavelengths of 1.55 micrometres). We also demonstrate coherent detection of a wavelength-division multiplexing data stream by using a pair of DKS frequency combs—one as a multi-wavelength light source at the transmitter and the other as the corresponding local oscillator at the receiver. This approach exploits the scalability of microresonator-based DKS frequency comb sources for massively parallel optical communications at both the transmitter and the receiver. Our results demonstrate the potential of these sources to replace the arrays of continuous-wave lasers that are currently used in high-speed communications. In combination with advanced spatial multiplexing schemes and highly integrated silicon photonic circuits, DKS frequency combs could bring chip-scale petabit-per-second transceivers into reach.

Method and Apparatus of Multiplexing and Acquiring Data from Multiple Optical Fibers Using a Single Data Channel of an Optical Frequency-Domain Reflectometry (OFDR) System

NASA Technical Reports Server (NTRS)

Parker, Jr., Allen R (Inventor); Chan, Hon Man (Inventor); Piazza, Anthony (Nino) (Inventor); Richards, William Lance (Inventor)

2014-01-01

A method and system for multiplexing a network of parallel fiber Bragg grating (FBG) sensor-fibers to a single acquisition channel of a closed Michelson interferometer system via a fiber splitter by distinguishing each branch of fiber sensors in the spatial domain. On each branch of the splitter, the fibers have a specific pre-determined length, effectively separating each branch of fiber sensors spatially. In the spatial domain the fiber branches are seen as part of one acquisition channel on the interrogation system. However, the FBG-reference arm beat frequency information for each fiber is retained. Since the beat frequency is generated between the reference arm, the effective fiber length of each successive branch includes the entire length of the preceding branch. The multiple branches are seen as one fiber having three segments where the segments can be resolved. This greatly simplifies optical, electronic and computational complexity, and is especially suited for use in multiplexed or branched OFS networks for SHM of large and/or distributed structures which need a lot of measurement points.

Mode division multiplexing using an orbital angular momentum mode sorter and MIMO-DSP over a graded-index few-mode optical fibre

PubMed Central

Huang, Hao; Milione, Giovanni; Lavery, Martin P. J.; Xie, Guodong; Ren, Yongxiong; Cao, Yinwen; Ahmed, Nisar; An Nguyen, Thien; Nolan, Daniel A.; Li, Ming-Jun; Tur, Moshe; Alfano, Robert R.; Willner, Alan E.

2015-01-01

Mode division multiplexing (MDM)– using a multimode optical fiber’s N spatial modes as data channels to transmit N independent data streams – has received interest as it can potentially increase optical fiber data transmission capacity N-times with respect to single mode optical fibers. Two challenges of MDM are (1) designing mode (de)multiplexers with high mode selectivity (2) designing mode (de)multiplexers without cascaded beam splitting’s 1/N insertion loss. One spatial mode basis that has received interest is that of orbital angular momentum (OAM) modes. In this paper, using a device referred to as an OAM mode sorter, we show that OAM modes can be (de)multiplexed over a multimode optical fiber with higher than −15 dB mode selectivity and without cascaded beam splitting’s 1/N insertion loss. As a proof of concept, the OAM modes of the LP11 mode group (OAM−1,0 and OAM+1,0), each carrying 20-Gbit/s polarization division multiplexed and quadrature phase shift keyed data streams, are transmitted 5km over a graded-index, few-mode optical fibre. Channel crosstalk is mitigated using 4 × 4 multiple-input-multiple-output digital-signal-processing with <1.5 dB power penalties at a bit-error-rate of 2 × 10−3. PMID:26450398

Mode division multiplexing using an orbital angular momentum mode sorter and MIMO-DSP over a graded-index few-mode optical fibre.

PubMed

Huang, Hao; Milione, Giovanni; Lavery, Martin P J; Xie, Guodong; Ren, Yongxiong; Cao, Yinwen; Ahmed, Nisar; An Nguyen, Thien; Nolan, Daniel A; Li, Ming-Jun; Tur, Moshe; Alfano, Robert R; Willner, Alan E

2015-10-09

Mode division multiplexing (MDM)- using a multimode optical fiber's N spatial modes as data channels to transmit N independent data streams - has received interest as it can potentially increase optical fiber data transmission capacity N-times with respect to single mode optical fibers. Two challenges of MDM are (1) designing mode (de)multiplexers with high mode selectivity (2) designing mode (de)multiplexers without cascaded beam splitting's 1/N insertion loss. One spatial mode basis that has received interest is that of orbital angular momentum (OAM) modes. In this paper, using a device referred to as an OAM mode sorter, we show that OAM modes can be (de)multiplexed over a multimode optical fiber with higher than -15 dB mode selectivity and without cascaded beam splitting's 1/N insertion loss. As a proof of concept, the OAM modes of the LP11 mode group (OAM-1,0 and OAM+1,0), each carrying 20-Gbit/s polarization division multiplexed and quadrature phase shift keyed data streams, are transmitted 5km over a graded-index, few-mode optical fibre. Channel crosstalk is mitigated using 4 × 4 multiple-input-multiple-output digital-signal-processing with <1.5 dB power penalties at a bit-error-rate of 2 × 10(-3).

Mode division multiplexing using an orbital angular momentum mode sorter and MIMO-DSP over a graded-index few-mode optical fibre

NASA Astrophysics Data System (ADS)

Huang, Hao; Milione, Giovanni; Lavery, Martin P. J.; Xie, Guodong; Ren, Yongxiong; Cao, Yinwen; Ahmed, Nisar; An Nguyen, Thien; Nolan, Daniel A.; Li, Ming-Jun; Tur, Moshe; Alfano, Robert R.; Willner, Alan E.

2015-10-01

Mode division multiplexing (MDM)- using a multimode optical fiber’s N spatial modes as data channels to transmit N independent data streams - has received interest as it can potentially increase optical fiber data transmission capacity N-times with respect to single mode optical fibers. Two challenges of MDM are (1) designing mode (de)multiplexers with high mode selectivity (2) designing mode (de)multiplexers without cascaded beam splitting’s 1/N insertion loss. One spatial mode basis that has received interest is that of orbital angular momentum (OAM) modes. In this paper, using a device referred to as an OAM mode sorter, we show that OAM modes can be (de)multiplexed over a multimode optical fiber with higher than -15 dB mode selectivity and without cascaded beam splitting’s 1/N insertion loss. As a proof of concept, the OAM modes of the LP11 mode group (OAM-1,0 and OAM+1,0), each carrying 20-Gbit/s polarization division multiplexed and quadrature phase shift keyed data streams, are transmitted 5km over a graded-index, few-mode optical fibre. Channel crosstalk is mitigated using 4 × 4 multiple-input-multiple-output digital-signal-processing with <1.5 dB power penalties at a bit-error-rate of 2 × 10-3.

Helms in Destiny laboratory with rack

NASA Image and Video Library

2001-05-07

ISS002-E-5859 (7 May 2001) --- Susan J. Helms, Expedition Two flight engineer, works on an Enhanced Space Station Multiplexer / Demultiplexer (ESSMDM) at the Maintenance Work Assembly (MWA) work surface in the Destiny module. The image was taken with a digital still camera.

Reconfigurable nanoscale spin-wave directional coupler

PubMed Central

Wang, Qi; Pirro, Philipp; Verba, Roman; Slavin, Andrei; Hillebrands, Burkard; Chumak, Andrii V.

2018-01-01

Spin waves, and their quanta magnons, are prospective data carriers in future signal processing systems because Gilbert damping associated with the spin-wave propagation can be made substantially lower than the Joule heat losses in electronic devices. Although individual spin-wave signal processing devices have been successfully developed, the challenging contemporary problem is the formation of two-dimensional planar integrated spin-wave circuits. Using both micromagnetic modeling and analytical theory, we present an effective solution of this problem based on the dipolar interaction between two laterally adjacent nanoscale spin-wave waveguides. The developed device based on this principle can work as a multifunctional and dynamically reconfigurable signal directional coupler performing the functions of a waveguide crossing element, tunable power splitter, frequency separator, or multiplexer. The proposed design of a spin-wave directional coupler can be used both in digital logic circuits intended for spin-wave computing and in analog microwave signal processing devices. PMID:29376117

Multidimensional signaling via wavelet packets

NASA Astrophysics Data System (ADS)

Lindsey, Alan R.

1995-04-01

This work presents a generalized signaling strategy for orthogonally multiplexed communication. Wavelet packet modulation (WPM) employs the basis functions from an arbitrary pruning of a full dyadic tree structured filter bank as orthogonal pulse shapes for conventional QAM symbols. The multi-scale modulation (MSM) and M-band wavelet modulation (MWM) schemes which have been recently introduced are handled as special cases, with the added benefit of an entire library of potentially superior sets of basis functions. The figures of merit are derived and it is shown that the power spectral density is equivalent to that for QAM (in fact, QAM is another special case) and hence directly applicable in existing systems employing this standard modulation. Two key advantages of this method are increased flexibility in time-frequency partitioning and an efficient all-digital filter bank implementation, making the WPM scheme more robust to a larger set of interferences (both temporal and sinusoidal) and computationally attractive as well.

Chaotic reconfigurable ZCMT precoder for OFDM data encryption and PAPR reduction

NASA Astrophysics Data System (ADS)

Chen, Han; Yang, Xuelin; Hu, Weisheng

2017-12-01

A secure orthogonal frequency division multiplexing (OFDM) transmission scheme precoded by chaotic Zadoff-Chu matrix transform (ZCMT) is proposed and demonstrated. It is proved that the reconfigurable ZCMT matrices after row/column permutations can be applied as an alternative precoder for peak-to-average power ratio (PAPR) reduction. The permutations and the reconfigurable parameters in ZCMT matrix are generated by a hyper digital chaos, in which a huge key space of ∼ 10800 is created for physical-layer OFDM data encryption. An encrypted data transmission of 8.9 Gb/s optical OFDM signals is successfully demonstrated over 20 km standard single-mode fiber (SSMF) for 16-QAM. The BER performance of the encrypted signals is improved by ∼ 2 dB (BER@ 10-3), which is mainly attributed to the effective reduction of PAPR via chaotic ZCMT precoding. Moreover, the chaotic ZCMT precoding scheme requires no sideband information, thus the spectrum efficiency is enhanced during transmission.

Reconfigurable nanoscale spin-wave directional coupler.

PubMed

Wang, Qi; Pirro, Philipp; Verba, Roman; Slavin, Andrei; Hillebrands, Burkard; Chumak, Andrii V

2018-01-01

Spin waves, and their quanta magnons, are prospective data carriers in future signal processing systems because Gilbert damping associated with the spin-wave propagation can be made substantially lower than the Joule heat losses in electronic devices. Although individual spin-wave signal processing devices have been successfully developed, the challenging contemporary problem is the formation of two-dimensional planar integrated spin-wave circuits. Using both micromagnetic modeling and analytical theory, we present an effective solution of this problem based on the dipolar interaction between two laterally adjacent nanoscale spin-wave waveguides. The developed device based on this principle can work as a multifunctional and dynamically reconfigurable signal directional coupler performing the functions of a waveguide crossing element, tunable power splitter, frequency separator, or multiplexer. The proposed design of a spin-wave directional coupler can be used both in digital logic circuits intended for spin-wave computing and in analog microwave signal processing devices.

Simple, multiplexed, PCR-based barcoding of DNA enables sensitive mutation detection in liquid biopsies using sequencing.

PubMed

Ståhlberg, Anders; Krzyzanowski, Paul M; Jackson, Jennifer B; Egyud, Matthew; Stein, Lincoln; Godfrey, Tony E

2016-06-20

Detection of cell-free DNA in liquid biopsies offers great potential for use in non-invasive prenatal testing and as a cancer biomarker. Fetal and tumor DNA fractions however can be extremely low in these samples and ultra-sensitive methods are required for their detection. Here, we report an extremely simple and fast method for introduction of barcodes into DNA libraries made from 5 ng of DNA. Barcoded adapter primers are designed with an oligonucleotide hairpin structure to protect the molecular barcodes during the first rounds of polymerase chain reaction (PCR) and prevent them from participating in mis-priming events. Our approach enables high-level multiplexing and next-generation sequencing library construction with flexible library content. We show that uniform libraries of 1-, 5-, 13- and 31-plex can be generated. Utilizing the barcodes to generate consensus reads for each original DNA molecule reduces background sequencing noise and allows detection of variant alleles below 0.1% frequency in clonal cell line DNA and in cell-free plasma DNA. Thus, our approach bridges the gap between the highly sensitive but specific capabilities of digital PCR, which only allows a limited number of variants to be analyzed, with the broad target capability of next-generation sequencing which traditionally lacks the sensitivity to detect rare variants. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Thermal Design and Characterization of Transition-Edge Sensor (TES) Bolometers for Frequency-Domain Multiplexing

NASA Astrophysics Data System (ADS)

Lueker, Martin; Benson, Bradford A.; Chang, Clarence L.; Cho, Hsiao-Mei; Dobbs, Matt; Holzapfel, William L.; Lanting, Trevor; Lee, Adrian T.; Mehl, Jared; Plagge, Thomas; Shirokoff, Erik; Spieler, Helmuth G.; Vieira, Joaquin D.

2009-06-01

In contemporary cosmic microwave background experiments, bolometric detectors are often background limited, and in this case the sensitivity of instruments can only be improved by increasing the number of background-limited detectors, and so contemporary TES receivers contain as many pixels as possible. Frequency-domain multiplexing (fMUX) is one strategy for reading out many detectors with one SQUID. For any readout system, it is important to carefully evaluate the thermal design of detector, in conjunction with the readout bandwidth, in order to ensure stable electro-thermal feedback (ETF). We demonstrate a novel technique for characterizing the thermal circuit of our detectors, using am AC-bias and the fMUX electronics. This technique is used to study the internal thermal coupling of a TES bolometer. We illustrate how the insights gathered by this technique have been instrumental in improving the stability of our multiplexed detectors for the south pole telescope (SPT).

Frequency-domain-independent vector analysis for mode-division multiplexed transmission

NASA Astrophysics Data System (ADS)

Liu, Yunhe; Hu, Guijun; Li, Jiao

2018-04-01

In this paper, we propose a demultiplexing method based on frequency-domain independent vector analysis (FD-IVA) algorithm for mode-division multiplexing (MDM) system. FD-IVA extends frequency-domain independent component analysis (FD-ICA) from unitary variable to multivariate variables, and provides an efficient method to eliminate the permutation ambiguity. In order to verify the performance of FD-IVA algorithm, a 6 ×6 MDM system is simulated. The simulation results show that the FD-IVA algorithm has basically the same bit-error-rate(BER) performance with the FD-ICA algorithm and frequency-domain least mean squares (FD-LMS) algorithm. Meanwhile, the convergence speed of FD-IVA algorithm is the same as that of FD-ICA. However, compared with the FD-ICA and the FD-LMS, the FD-IVA has an obviously lower computational complexity.

Frequency multiplexed flux locked loop architecture providing an array of DC SQUIDS having both shared and unshared components

DOEpatents

Ganther, Jr., Kenneth R.; Snapp, Lowell D.

2002-01-01

Architecture for frequency multiplexing multiple flux locked loops in a system comprising an array of DC SQUID sensors. The architecture involves dividing the traditional flux locked loop into multiple unshared components and a single shared component which, in operation, form a complete flux locked loop relative to each DC SQUID sensor. Each unshared flux locked loop component operates on a different flux modulation frequency. The architecture of the present invention allows a reduction from 2N to N+1 in the number of connections between the cryogenic DC SQUID sensors and their associated room temperature flux locked loops. Furthermore, the 1.times.N architecture of the present invention can be paralleled to form an M.times.N array architecture without increasing the required number of flux modulation frequencies.

Analysis of secured Optical Orthogonal Frequency Division Multiplexed System

NASA Astrophysics Data System (ADS)

Gill, Harsimranjit Singh; Bhatia, Kamaljit Singh; Gill, Sandeep Singh

2017-05-01

In this paper, security issues for optical orthogonal frequency division multiplexed (OFDM) systems are emphasized. The encryption has been done on the data of coded OFDM symbols using data encryption standard (DES) algorithm before transmitting through the fiber. The results obtained justify that the DES provides better security to the input data without further bandwidth requirement. The data is transmitted to a distance of 1,000 km in a single-mode fiber with 16-quadrature amplitude modulation. The peak-to-average power ratio and optical signal-to-noise ratio of secure coded OFDM signal is fairly better than the conventional OFDM signal.

Frequency-multiplexed bias and readout of a 16-pixel superconducting nanowire single-photon detector array

NASA Astrophysics Data System (ADS)

Doerner, S.; Kuzmin, A.; Wuensch, S.; Charaev, I.; Boes, F.; Zwick, T.; Siegel, M.

2017-07-01

We demonstrate a 16-pixel array of microwave-current driven superconducting nanowire single-photon detectors with an integrated and scalable frequency-division multiplexing architecture, which reduces the required number of bias and readout lines to a single microwave feed line. The electrical behavior of the photon-sensitive nanowires, embedded in a resonant circuit, as well as the optical performance and timing jitter of the single detectors is discussed. Besides the single pixel measurements, we also demonstrate the operation of a 16-pixel array with a temporal, spatial, and photon-number resolution.

Fast ultra-wideband microwave spectral scanning utilizing photonic wavelength- and time-division multiplexing.

PubMed

Li, Yihan; Kuse, Naoya; Fermann, Martin

2017-08-07

A high-speed ultra-wideband microwave spectral scanning system is proposed and experimentally demonstrated. Utilizing coherent dual electro-optical frequency combs and a recirculating optical frequency shifter, the proposed system realizes wavelength- and time-division multiplexing at the same time, offering flexibility between scan speed and size, weight and power requirements (SWaP). High-speed spectral scanning spanning from ~1 to 8 GHz with ~1.2 MHz spectral resolution is achieved experimentally within 14 µs. The system can be easily scaled to higher bandwidth coverage, faster scanning speed or finer spectral resolution with suitable hardware.

Optimising the multiplex factor of the frequency domain multiplexed readout of the TES-based microcalorimeter imaging array for the X-IFU instrument on the Athena x-ray observatory

NASA Astrophysics Data System (ADS)

van der Kuur, J.; Gottardi, L. G.; Akamatsu, H.; van Leeuwen, B. J.; den Hartog, R.; Haas, D.; Kiviranta, M.; Jackson, B. J.

2016-07-01

Athena is a space-based X-ray observatory intended for exploration of the hot and energetic universe. One of the science instruments on Athena will be the X-ray Integrated Field Unit (X-IFU), which is a cryogenic X-ray spectrometer, based on a large cryogenic imaging array of Transition Edge Sensors (TES) based microcalorimeters operating at a temperature of 100mK. The imaging array consists of 3800 pixels providing 2.5 eV spectral resolution, and covers a field of view with a diameter of of 5 arc minutes. Multiplexed readout of the cryogenic microcalorimeter array is essential to comply with the cooling power and complexity constraints on a space craft. Frequency domain multiplexing has been under development for the readout of TES-based detectors for this purpose, not only for the X-IFU detector arrays but also for TES-based bolometer arrays for the Safari instrument of the Japanese SPICA observatory. This paper discusses the design considerations which are applicable to optimise the multiplex factor within the boundary conditions as set by the space craft. More specifically, the interplay between the science requirements such as pixel dynamic range, pixel speed, and cross talk, and the space craft requirements such as the power dissipation budget, available bandwidth, and electromagnetic compatibility will be discussed.

Frequency and bandwidth conversion of single photons in a room-temperature diamond quantum memory

PubMed Central

Fisher, Kent A. G.; England, Duncan G.; MacLean, Jean-Philippe W.; Bustard, Philip J.; Resch, Kevin J.; Sussman, Benjamin J.

2016-01-01

The spectral manipulation of photons is essential for linking components in a quantum network. Large frequency shifts are needed for conversion between optical and telecommunication frequencies, while smaller shifts are useful for frequency-multiplexing quantum systems, in the same way that wavelength division multiplexing is used in classical communications. Here we demonstrate frequency and bandwidth conversion of single photons in a room-temperature diamond quantum memory. Heralded 723.5 nm photons, with 4.1 nm bandwidth, are stored as optical phonons in the diamond via a Raman transition. Upon retrieval from the diamond memory, the spectral shape of the photons is determined by a tunable read pulse through the reverse Raman transition. We report central frequency tunability over 4.2 times the input bandwidth, and bandwidth modulation between 0.5 and 1.9 times the input bandwidth. Our results demonstrate the potential for diamond, and Raman memories in general, as an integrated platform for photon storage and spectral conversion. PMID:27045988

Frequency and bandwidth conversion of single photons in a room-temperature diamond quantum memory.

PubMed

Fisher, Kent A G; England, Duncan G; MacLean, Jean-Philippe W; Bustard, Philip J; Resch, Kevin J; Sussman, Benjamin J

2016-04-05

The spectral manipulation of photons is essential for linking components in a quantum network. Large frequency shifts are needed for conversion between optical and telecommunication frequencies, while smaller shifts are useful for frequency-multiplexing quantum systems, in the same way that wavelength division multiplexing is used in classical communications. Here we demonstrate frequency and bandwidth conversion of single photons in a room-temperature diamond quantum memory. Heralded 723.5 nm photons, with 4.1 nm bandwidth, are stored as optical phonons in the diamond via a Raman transition. Upon retrieval from the diamond memory, the spectral shape of the photons is determined by a tunable read pulse through the reverse Raman transition. We report central frequency tunability over 4.2 times the input bandwidth, and bandwidth modulation between 0.5 and 1.9 times the input bandwidth. Our results demonstrate the potential for diamond, and Raman memories in general, as an integrated platform for photon storage and spectral conversion.

Ultrasensitive Single Fluorescence-Labeled Probe-Mediated Single Universal Primer-Multiplex-Droplet Digital Polymerase Chain Reaction for High-Throughput Genetically Modified Organism Screening.

PubMed

Niu, Chenqi; Xu, Yuancong; Zhang, Chao; Zhu, Pengyu; Huang, Kunlun; Luo, Yunbo; Xu, Wentao

2018-05-01

As genetically modified (GM) technology develops and genetically modified organisms (GMOs) become more available, GMOs face increasing regulations and pressure to adhere to strict labeling guidelines. A singleplex detection method cannot perform the high-throughput analysis necessary for optimal GMO detection. Combining the advantages of multiplex detection and droplet digital polymerase chain reaction (ddPCR), a single universal primer-multiplex-ddPCR (SUP-M-ddPCR) strategy was proposed for accurate broad-spectrum screening and quantification. The SUP increases efficiency of the primers in PCR and plays an important role in establishing a high-throughput, multiplex detection method. Emerging ddPCR technology has been used for accurate quantification of nucleic acid molecules without a standard curve. Using maize as a reference point, four heterologous sequences ( 35S, NOS, NPTII, and PAT) were selected to evaluate the feasibility and applicability of this strategy. Surprisingly, these four genes cover more than 93% of the transgenic maize lines and serve as preliminary screening sequences. All screening probes were labeled with FAM fluorescence, which allows the signals from the samples with GMO content and those without to be easily differentiated. This fiveplex screening method is a new development in GMO screening. Utilizing an optimal amplification assay, the specificity, limit of detection (LOD), and limit of quantitation (LOQ) were validated. The LOD and LOQ of this GMO screening method were 0.1% and 0.01%, respectively, with a relative standard deviation (RSD) < 25%. This method could serve as an important tool for the detection of GM maize from different processed, commercially available products. Further, this screening method could be applied to other fields that require reliable and sensitive detection of DNA targets.

Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR.

PubMed

Zhong, Qun; Bhattacharya, Smiti; Kotsopoulos, Steven; Olson, Jeff; Taly, Valérie; Griffiths, Andrew D; Link, Darren R; Larson, Jonathan W

2011-07-07

Quantitative polymerase chain reactions (qPCR) based on real-time PCR constitute a powerful and sensitive method for the analysis of nucleic acids. However, in qPCR, the ability to multiplex targets using differently colored fluorescent probes is typically limited to 4-fold by the spectral overlap of the fluorophores. Furthermore, multiplexing qPCR assays requires expensive instrumentation and most often lengthy assay development cycles. Digital PCR (dPCR), which is based on the amplification of single target DNA molecules in many separate reactions, is an attractive alternative to qPCR. Here we report a novel and easy method for multiplexing dPCR in picolitre droplets within emulsions-generated and read out in microfluidic devices-that takes advantage of both the very high numbers of reactions possible within emulsions (>10(6)) as well as the high likelihood that the amplification of only a single target DNA molecule will initiate within each droplet. By varying the concentration of different fluorogenic probes of the same color, it is possible to identify the different probes on the basis of fluorescence intensity. Adding multiple colors increases the number of possible reactions geometrically, rather than linearly as with qPCR. Accurate and precise copy numbers of up to sixteen per cell were measured using a model system. A 5-plex assay for spinal muscular atrophy was demonstrated with just two fluorophores to simultaneously measure the copy number of two genes (SMN1 and SMN2) and to genotype a single nucleotide polymorphism (c.815A>G, SMN1). Results of a pilot study with SMA patients are presented. This journal is © The Royal Society of Chemistry 2011

Screening newborn blood spots for 22q11.2 deletion syndrome using multiplex droplet digital PCR.

PubMed

Pretto, Dalyir; Maar, Dianna; Yrigollen, Carolyn M; Regan, Jack; Tassone, Flora

2015-01-01

The diagnosis of 22q11 deletion syndrome (22q11DS) is often delayed or missed due to the wide spectrum of clinical involvement ranging from mild to severe, often life-threatening conditions. A delayed diagnosis can lead to life-long health issues that could be ameliorated with early intervention and treatment. Owing to the high impact of 22q11DS on public health, propositions have been made to include 22q11DS in newborn screening panels; however, the method of choice for detecting 22q11DS, fluorescent in situ hybridization, requires specialized equipment and is cumbersome for most laboratories to implement as part of their routine screening. We sought to develop a new genetic screen for 22q11DS that is rapid, cost-effective, and easily used by laboratories currently performing newborn screening. We evaluated the accuracy of multiplex droplet digital PCR (ddPCR) in the detection of copy number of 22q11DS by screening samples from 26 patients with 22q11DS blindly intermixed with 1096 blood spot cards from the general population (total n = 1122). Multiplex ddPCR correctly identified all 22q11DS samples and distinguished between 1.5- and 3-Mb deletions, suggesting the approach is sensitive and specific for the detection of 22q11DS. These data demonstrate the utility of multiplex ddPCR for large-scale population-based studies that screen for 22q11DS. The use of samples from blood spot cards suggests that this approach has promise for newborn screening of 22q11DS, and potentially for other microdeletion syndromes, for which early detection can positively impact clinical outcome for those affected. © 2014 American Association for Clinical Chemistry.

High content screening of ToxCast compounds using Vala Sciences’ complex cell culturing systems (SOT)

EPA Science Inventory

US EPA’s ToxCast research program evaluates bioactivity for thousands of chemicals utilizing high-throughput screening assays to inform chemical testing decisions. Vala Sciences provides high content, multiplexed assays that utilize quantitative cell-based digital image analysis....

Hierarchical patch-based co-registration of differently stained histopathology slides

NASA Astrophysics Data System (ADS)

Yigitsoy, Mehmet; Schmidt, Günter

2017-03-01

Over the past decades, digital pathology has emerged as an alternative way of looking at the tissue at subcellular level. It enables multiplexed analysis of different cell types at micron level. Information about cell types can be extracted by staining sections of a tissue block using different markers. However, robust fusion of structural and functional information from different stains is necessary for reproducible multiplexed analysis. Such a fusion can be obtained via image co-registration by establishing spatial correspondences between tissue sections. Spatial correspondences can then be used to transfer various statistics about cell types between sections. However, the multi-modal nature of images and sparse distribution of interesting cell types pose several challenges for the registration of differently stained tissue sections. In this work, we propose a co-registration framework that efficiently addresses such challenges. We present a hierarchical patch-based registration of intensity normalized tissue sections. Preliminary experiments demonstrate the potential of the proposed technique for the fusion of multi-modal information from differently stained digital histopathology sections.

Multiplex DNA detection of food allergens on a digital versatile disk.

PubMed

Tortajada-Genaro, Luis A; Santiago-Felipe, Sara; Morais, Sergi; Gabaldón, José Antonio; Puchades, Rosa; Maquieira, Ángel

2012-01-11

The development of a DNA microarray method on a digital versatile disk (DVD) is described for the simultaneous detection of traces of hazelnut ( Corylus avellana L.), peanut ( Arachis hypogaea ), and soybean ( Glycine max ) in foods. After DNA extraction, multiplex PCR was set up using 5'-labeled specific primers for Cor a 1, Ar h 2, and Le genes, respectively. Digoxin-labeled PCR products were detected by hybridization with 5'-biotinylated probes immobilized on a streptavidin-modified DVD surface. The reaction product attenuates the signal intensity of the laser that reached the DVD drive used as detector, correlating well with the amount of amplified sequence. Analytical performances showed a detection limit of 1 μg/g and good assay reproducibility (RSD 8%), suitable for the simultaneous detection of the three targeted allergens. The developed methodology was tested with several commercially available foodstuffs, demonstrating its applicability. The results were in good agreement, in terms of sensitivity and reproducibility, with those obtained with ELISA, PCR-gel agarose electrophoresis, and RT-PCR.

The readout chain for the bar PANDA MVD strip detector

NASA Astrophysics Data System (ADS)

Schnell, R.; Brinkmann, K.-Th.; Di Pietro, V.; Kleines, H.; Goerres, A.; Riccardi, A.; Rivetti, A.; Rolo, M. D.; Sohlbach, H.; Zaunick, H.-G.

2015-02-01

The bar PANDA (antiProton ANnihilation at DArmstadt) experiment will study the strong interaction in annihilation reactions between an antiproton beam and a stationary gas jet target. The detector will comprise different sub-detectors for tracking, particle identification and calorimetry. The Micro-Vertex Detector (MVD) as the innermost part of the tracking system will allow precise tracking and detection of secondary vertices. For the readout of the double-sided silicon strip sensors a custom-made ASIC is being developed, employing the Time-over-Threshold (ToT) technique for digitization and utilize time-to-digital converters (TDC) to provide a high-precision time stamp of the hit. A custom-made Module Data Concentrator ASIC (MDC) will multiplex the data of all front-ends of one sensor towards the CERN-developed GBT chip set (GigaBit Transceiver). The MicroTCA-based MVD Multiplexer Board (MMB) at the off-detector site will receive and concentrate the data from the GBT links and transfer it to FPGA-based compute nodes for global event building.

A new IPQAM modulator with high integrated degree for digital TV

NASA Astrophysics Data System (ADS)

He, Yejun; Liu, Deming; Zhu, Guangxi; Jiang, Tao; Sun, Gongxian

2008-12-01

As video on demand (VOD) services are deployed, cable operators will experience a fundamental shift in their business, moving from broadcast to unicast content delivery. Another significant change is the introduction of Gigabit Ethernet into their network, which is providing an unprecedented opportunity to turn the cable operator's infrastructure into a sustainable competitive advantage. However, Gigabit Ethernet is more than just transport; it's the foundation of the Next-Generation Digital Video Network. IPQAM modulator, which is a main equipment, aren't made in China so far. It is the first time that we did design IPQAM modulator and will apply it to interactive TV based on DWDM (dense wavelength-division multiplexing). This paper introduces the principle of IPQAM modulator and transmission approach. The differences between IPQAM and conventional QAM are analysed. Some key techniques such as scrambling, statistical multiplexing, Data over Cable Service Interface Specification (DOCSIS) 3.0, software defined radio as well as DVB simulcrypt are also studied.

Data multiplexing in radio interferometric calibration

NASA Astrophysics Data System (ADS)

Yatawatta, Sarod; Diblen, Faruk; Spreeuw, Hanno; Koopmans, L. V. E.

2018-03-01

New and upcoming radio interferometers will produce unprecedented amount of data that demand extremely powerful computers for processing. This is a limiting factor due to the large computational power and energy costs involved. Such limitations restrict several key data processing steps in radio interferometry. One such step is calibration where systematic errors in the data are determined and corrected. Accurate calibration is an essential component in reaching many scientific goals in radio astronomy and the use of consensus optimization that exploits the continuity of systematic errors across frequency significantly improves calibration accuracy. In order to reach full consensus, data at all frequencies need to be calibrated simultaneously. In the SKA regime, this can become intractable if the available compute agents do not have the resources to process data from all frequency channels simultaneously. In this paper, we propose a multiplexing scheme that is based on the alternating direction method of multipliers with cyclic updates. With this scheme, it is possible to simultaneously calibrate the full data set using far fewer compute agents than the number of frequencies at which data are available. We give simulation results to show the feasibility of the proposed multiplexing scheme in simultaneously calibrating a full data set when a limited number of compute agents are available.

Digital microfluidic platform for multiplexing enzyme assays: implications for lysosomal storage disease screening in newborns.

PubMed

Sista, Ramakrishna S; Eckhardt, Allen E; Wang, Tong; Graham, Carrie; Rouse, Jeremy L; Norton, Scott M; Srinivasan, Vijay; Pollack, Michael G; Tolun, Adviye A; Bali, Deeksha; Millington, David S; Pamula, Vamsee K

2011-10-01

Newborn screening for lysosomal storage diseases (LSDs) has been gaining considerable interest owing to the availability of enzyme replacement therapies. We present a digital microfluidic platform to perform rapid, multiplexed enzymatic analysis of acid α-glucosidase (GAA) and acid α-galactosidase to screen for Pompe and Fabry disorders. The results were compared with those obtained using standard fluorometric methods. We performed bench-based, fluorometric enzymatic analysis on 60 deidentified newborn dried blood spots (DBSs), plus 10 Pompe-affected and 11 Fabry-affected samples, at Duke Biochemical Genetics Laboratory using a 3-mm punch for each assay and an incubation time of 20 h. We used a digital microfluidic platform to automate fluorometric enzymatic assays at Advanced Liquid Logic Inc. using extract from a single punch for both assays, with an incubation time of 6 h. Assays were also performed with an incubation time of 1 h. Assay results were generally comparable, although mean enzymatic activity for GAA using microfluidics was approximately 3 times higher than that obtained using bench-based methods, which could be attributed to higher substrate concentration. Clear separation was observed between the normal and affected samples at both 6- and 1-h incubation times using digital microfluidics. A digital microfluidic platform compared favorably with a clinical reference laboratory to perform enzymatic analysis in DBSs for Pompe and Fabry disorders. This platform presents a new technology for a newborn screening laboratory to screen LSDs by fully automating all the liquid-handling operations in an inexpensive system, providing rapid results.

High-Speed Digital Interferometry

NASA Technical Reports Server (NTRS)

De Vine, Glenn; Shaddock, Daniel A.; Ware, Brent; Spero, Robert E.; Wuchenich, Danielle M.; Klipstein, William M.; McKenzie, Kirk

2012-01-01

Digitally enhanced heterodyne interferometry (DI) is a laser metrology technique employing pseudo-random noise (PRN) codes phase-modulated onto an optical carrier. Combined with heterodyne interferometry, the PRN code is used to select individual signals, returning the inherent interferometric sensitivity determined by the optical wavelength. The signal isolation arises from the autocorrelation properties of the PRN code, enabling both rejection of spurious signals (e.g., from scattered light) and multiplexing capability using a single metrology system. The minimum separation of optical components is determined by the wavelength of the PRN code.

Optical Security System Based on the Biometrics Using Holographic Storage Technique with a Simple Data Format

NASA Astrophysics Data System (ADS)

Jun, An Won

2006-01-01

We implement a first practical holographic security system using electrical biometrics that combines optical encryption and digital holographic memory technologies. Optical information for identification includes a picture of face, a name, and a fingerprint, which has been spatially multiplexed by random phase mask used for a decryption key. For decryption in our biometric security system, a bit-error-detection method that compares the digital bit of live fingerprint with of fingerprint information extracted from hologram is used.

Digital transplantation pathology: combining whole slide imaging, multiplex staining and automated image analysis.

PubMed

Isse, K; Lesniak, A; Grama, K; Roysam, B; Minervini, M I; Demetris, A J

2012-01-01

Conventional histopathology is the gold standard for allograft monitoring, but its value proposition is increasingly questioned. "-Omics" analysis of tissues, peripheral blood and fluids and targeted serologic studies provide mechanistic insights into allograft injury not currently provided by conventional histology. Microscopic biopsy analysis, however, provides valuable and unique information: (a) spatial-temporal relationships; (b) rare events/cells; (c) complex structural context; and (d) integration into a "systems" model. Nevertheless, except for immunostaining, no transformative advancements have "modernized" routine microscopy in over 100 years. Pathologists now team with hardware and software engineers to exploit remarkable developments in digital imaging, nanoparticle multiplex staining, and computational image analysis software to bridge the traditional histology-global "-omic" analyses gap. Included are side-by-side comparisons, objective biopsy finding quantification, multiplexing, automated image analysis, and electronic data and resource sharing. Current utilization for teaching, quality assurance, conferencing, consultations, research and clinical trials is evolving toward implementation for low-volume, high-complexity clinical services like transplantation pathology. Cost, complexities of implementation, fluid/evolving standards, and unsettled medical/legal and regulatory issues remain as challenges. Regardless, challenges will be overcome and these technologies will enable transplant pathologists to increase information extraction from tissue specimens and contribute to cross-platform biomarker discovery for improved outcomes. ©Copyright 2011 The American Society of Transplantation and the American Society of Transplant Surgeons.

Real-time, multiplexed electrochemical DNA detection using an active complementary metal-oxide-semiconductor biosensor array with integrated sensor electronics.

PubMed

Levine, Peter M; Gong, Ping; Levicky, Rastislav; Shepard, Kenneth L

2009-03-15

Optical biosensing based on fluorescence detection has arguably become the standard technique for quantifying extents of hybridization between surface-immobilized probes and fluorophore-labeled analyte targets in DNA microarrays. However, electrochemical detection techniques are emerging which could eliminate the need for physically bulky optical instrumentation, enabling the design of portable devices for point-of-care applications. Unlike fluorescence detection, which can function well using a passive substrate (one without integrated electronics), multiplexed electrochemical detection requires an electronically active substrate to analyze each array site and benefits from the addition of integrated electronic instrumentation to further reduce platform size and eliminate the electromagnetic interference that can result from bringing non-amplified signals off chip. We report on an active electrochemical biosensor array, constructed with a standard complementary metal-oxide-semiconductor (CMOS) technology, to perform quantitative DNA hybridization detection on chip using targets conjugated with ferrocene redox labels. A 4 x 4 array of gold working electrodes and integrated potentiostat electronics, consisting of control amplifiers and current-input analog-to-digital converters, on a custom-designed 5 mm x 3 mm CMOS chip drive redox reactions using cyclic voltammetry, sense DNA binding, and transmit digital data off chip for analysis. We demonstrate multiplexed and specific detection of DNA targets as well as real-time monitoring of hybridization, a task that is difficult, if not impossible, with traditional fluorescence-based microarrays.

Development and evaluation of a Hadamard transform imaging spectrometer and a Hadamard transform thermal imager

NASA Technical Reports Server (NTRS)

Harwit, M.; Swift, R.; Wattson, R.; Decker, J.; Paganetti, R.

1976-01-01

A spectrometric imager and a thermal imager, which achieve multiplexing by the use of binary optical encoding masks, were developed. The masks are based on orthogonal, pseudorandom digital codes derived from Hadamard matrices. Spatial and/or spectral data is obtained in the form of a Hadamard transform of the spatial and/or spectral scene; computer algorithms are then used to decode the data and reconstruct images of the original scene. The hardware, algorithms and processing/display facility are described. A number of spatial and spatial/spectral images are presented. The achievement of a signal-to-noise improvement due to the signal multiplexing was also demonstrated. An analysis of the results indicates both the situations for which the multiplex advantage may be gained, and the limitations of the technique. A number of potential applications of the spectrometric imager are discussed.

High-Definition Optical Velocimetry: A New Diagnostic Paradigm for Nuclear Security

DOE Office of Scientific and Technical Information (OSTI.GOV)

Daykin, E; Diaz, A; Gallegos, C

This slide-show describes work done to address the challenge of high-definition optical velocimetry with hundred(s) of high-fidelity velocity vs. time measurements. After a review of the historical context and a general technical description of how optical velocimetry, particularly photonic Doppler velocimetry, works, the innovation of multiplexed photonic Doppler velocimetry (MPDV) is described as implemented with commercially available telecom products and dense wavelength division multiplexing (DWDM). High amplification of small signals allows for laser-safe operations. The authors have evaluated and leveraged telecom components– optical amplifiers, wavelength multiplexers, and seed lasers–to provide an economical, compact and rugged approach to system architecture. Fouriermore » transform data analysis is seen to be robust and capable of discriminating simultaneous data traces recorded onto a single digitizer channel. The authors successfully fielded demonstration MPDV system on shock driven experiments.« less

Automated attendance accounting system

NASA Technical Reports Server (NTRS)

Chapman, C. P. (Inventor)

1973-01-01

An automated accounting system useful for applying data to a computer from any or all of a multiplicity of data terminals is disclosed. The system essentially includes a preselected number of data terminals which are each adapted to convert data words of decimal form to another form, i.e., binary, usable with the computer. Each data terminal may take the form of a keyboard unit having a number of depressable buttons or switches corresponding to selected data digits and/or function digits. A bank of data buffers, one of which is associated with each data terminal, is provided as a temporary storage. Data from the terminals is applied to the data buffers on a digit by digit basis for transfer via a multiplexer to the computer.

Free-space optical polarization demultiplexing and multiplexing by means of conical refraction.

PubMed

Turpin, Alex; Loiko, Yurii; Kalkandjiev, Todor K; Mompart, Jordi

2012-10-15

Polarization demultiplexing and multiplexing by means of conical refraction is proposed to increase the channel capacity for free-space optical communication applications. The proposed technique is based on the forward-backward optical transform occurring when a light beam propagates consecutively along the optic axes of two identical biaxial crystals with opposite orientations of their conical refraction characteristic vectors. We present an experimental proof of usefulness of the conical refraction demultiplexing and multiplexing technique by increasing in one order of magnitude the channel capacity at optical frequencies in a propagation distance of 4 m.

Time multiplexing based extended depth of focus imaging.

PubMed

Ilovitsh, Asaf; Zalevsky, Zeev

2016-01-01

We propose to utilize the time multiplexing super resolution method to extend the depth of focus of an imaging system. In standard time multiplexing, the super resolution is achieved by generating duplication of the optical transfer function in the spectrum domain, by the use of moving gratings. While this improves the spatial resolution, it does not increase the depth of focus. By changing the gratings frequency and, by that changing the duplication positions, it is possible to obtain an extended depth of focus. The proposed method is presented analytically, demonstrated via numerical simulations and validated by a laboratory experiment.

Multi-wavelengths digital holography: reconstruction, synthesis and display of holograms using adaptive transformation.

PubMed

Memmolo, P; Finizio, A; Paturzo, M; Ferraro, P; Javidi, B

2012-05-01

A method based on spatial transformations of multiwavelength digital holograms and the correlation matching of their numerical reconstructions is proposed, with the aim to improve superimposition of different color reconstructed images. This method is based on an adaptive affine transform of the hologram that permits management of the physical parameters of numerical reconstruction. In addition, we present a procedure to synthesize a single digital hologram in which three different colors are multiplexed. The optical reconstruction of the synthetic hologram by a spatial light modulator at one wavelength allows us to display all color features of the object, avoiding loss of details.

Data system for multiplexed water-current meters

NASA Technical Reports Server (NTRS)

Ramsey, C. R.

1977-01-01

Flow rates at 32 flood plain locations are measured simultaneously by single digital logic unit with high noise immunity. Water flowing through pygmy current meters rotates element that closes electrical contact once every resolution, so flow rate is measured by counting number of closures in time interval.

Noise-free recovery of optodigital encrypted and multiplexed images.

PubMed

Henao, Rodrigo; Rueda, Edgar; Barrera, John F; Torroba, Roberto

2010-02-01

We present a method that allows storing multiple encrypted data using digital holography and a joint transform correlator architecture with a controllable angle reference wave. In this method, the information is multiplexed by using a key and a different reference wave angle for each object. In the recovering process, the use of different reference wave angles prevents noise produced by the nonrecovered objects from being superimposed on the recovered object; moreover, the position of the recovered object in the exit plane can be fully controlled. We present the theoretical analysis and the experimental results that show the potential and applicability of the method.

Coherent UDWDM PON with joint subcarrier reception at OLT.

PubMed

Kottke, Christoph; Fischer, Johannes Karl; Elschner, Robert; Frey, Felix; Hilt, Jonas; Schubert, Colja; Schmidt, Daniel; Wu, Zifeng; Lankl, Berthold

2014-07-14

In this contribution, we report on the experimental investigation of an ultra-dense wavelength-division multiplexing (UDWDM) upstream link with up to 700 × 2.488 Gb/s polarization-division multiplexing differential quadrature phase-shift keying parallel upstream user channels transmitted over 80 km of standard single-mode fiber. We discuss challenges of the digital signal processing in the optical line terminal arising from the joint reception of several upstream user channels. We present solutions for resource and cost-efficient realization of the required channel separation, matched filtering, down-conversion and decimation as well as realization of the clock recovery and polarization demultiplexing for each individual channel.

Analog nonlinear MIMO receiver for optical mode division multiplexing transmission.

PubMed

Spalvieri, Arnaldo; Boffi, Pierpaolo; Pecorino, Simone; Barletta, Luca; Magarini, Maurizio; Gatto, Alberto; Martelli, Paolo; Martinelli, Mario

2013-10-21

The complexity and the power consumption of digital signal processing are crucial issues in optical transmission systems based on mode division multiplexing and coherent multiple-input multiple-output (MIMO) processing at the receiver. In this paper the inherent characteristic of spatial separation between fiber modes is exploited, getting a MIMO system where joint demultiplexing and detection is based on spatially separated photodetectors. After photodetection, one has a MIMO system with nonlinear crosstalk between modes. The paper shows that the nonlinear crosstalk can be dealt with by a low-complexity and non-adaptive detection scheme, at least in the cases presented in the paper.

Optical delay encoding for fast timing and detector signal multiplexing in PET

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grant, Alexander M.; Levin, Craig S., E-mail: cslevin@stanford.edu; Molecular Imaging Program at Stanford

2015-08-15

Purpose: The large number of detector channels in modern positron emission tomography (PET) scanners poses a challenge in terms of readout electronics complexity. Multiplexing schemes are typically implemented to reduce the number of physical readout channels, but often result in performance degradation. Novel methods of multiplexing in PET must be developed to avoid this data degradation. The preservation of fast timing information is especially important for time-of-flight PET. Methods: A new multiplexing scheme based on encoding detector interaction events with a series of extremely fast overlapping optical pulses with precise delays is demonstrated in this work. Encoding events in thismore » way potentially allows many detector channels to be simultaneously encoded onto a single optical fiber that is then read out by a single digitizer. A two channel silicon photomultiplier-based prototype utilizing this optical delay encoding technique along with dual threshold time-over-threshold is demonstrated. Results: The optical encoding and multiplexing prototype achieves a coincidence time resolution of 160 ps full width at half maximum (FWHM) and an energy resolution of 13.1% FWHM at 511 keV with 3 × 3 × 5 mm{sup 3} LYSO crystals. All interaction information for both detectors, including timing, energy, and channel identification, is encoded onto a single optical fiber with little degradation. Conclusions: Optical delay encoding and multiplexing technology could lead to time-of-flight PET scanners with fewer readout channels and simplified data acquisition systems.« less

A Novel Cost-effective OFDM WDM-PON Radio Over Fiber System Employing FBG to Generate Optical mm-wave

NASA Astrophysics Data System (ADS)

Nguyen, HoangViet

2015-03-01

We have investigated and demonstrated a novel scheme to generate 2.5 Gbit/s 64 QAM orthogonal frequency division multiplexing (OFDM) signals for Radio Over Fiber (ROF) systems. We employ Fiber Bragg Grating (FBG) because the repetitive frequency of the RF source and the bandwidth of the optical modulator are largely reduced and the architecture of the ROF system is simpler. Wavelength-Division-Multiplexed Passive Optical Network (WDM-PON) has been considered as a promising solution for future broadband access networks. Principle of WDM-PON access network compatible with OFDM-ROF systems is investigated. This novel scheme which has multiple double-frequency technique to generate mm-wave signal to carry OFDM signals is a practical scheme to be applied for future broadband access networks.

A highly accurate wireless digital sun sensor based on profile detecting and detector multiplexing technologies

NASA Astrophysics Data System (ADS)

Wei, Minsong; Xing, Fei; You, Zheng

2017-01-01

The advancing growth of micro- and nano-satellites requires miniaturized sun sensors which could be conveniently applied in the attitude determination subsystem. In this work, a profile detecting technology based high accurate wireless digital sun sensor was proposed, which could transform a two-dimensional image into two-linear profile output so that it can realize a high update rate under a very low power consumption. A multiple spots recovery approach with an asymmetric mask pattern design principle was introduced to fit the multiplexing image detector method for accuracy improvement of the sun sensor within a large Field of View (FOV). A FOV determination principle based on the concept of FOV region was also proposed to facilitate both sub-FOV analysis and the whole FOV determination. A RF MCU, together with solar cells, was utilized to achieve the wireless and self-powered functionality. The prototype of the sun sensor is approximately 10 times lower in size and weight compared with the conventional digital sun sensor (DSS). Test results indicated that the accuracy of the prototype was 0.01° within a cone FOV of 100°. Such an autonomous DSS could be equipped flexibly on a micro- or nano-satellite, especially for highly accurate remote sensing applications.

Digital Signal Processing For Low Bit Rate TV Image Codecs

NASA Astrophysics Data System (ADS)

Rao, K. R.

1987-06-01

In view of the 56 KBPS digital switched network services and the ISDN, low bit rate codecs for providing real time full motion color video are under various stages of development. Some companies have already brought the codecs into the market. They are being used by industry and some Federal Agencies for video teleconferencing. In general, these codecs have various features such as multiplexing audio and data, high resolution graphics, encryption, error detection and correction, self diagnostics, freezeframe, split video, text overlay etc. To transmit the original color video on a 56 KBPS network requires bit rate reduction of the order of 1400:1. Such a large scale bandwidth compression can be realized only by implementing a number of sophisticated,digital signal processing techniques. This paper provides an overview of such techniques and outlines the newer concepts that are being investigated. Before resorting to the data compression techniques, various preprocessing operations such as noise filtering, composite-component transformation and horizontal and vertical blanking interval removal are to be implemented. Invariably spatio-temporal subsampling is achieved by appropriate filtering. Transform and/or prediction coupled with motion estimation and strengthened by adaptive features are some of the tools in the arsenal of the data reduction methods. Other essential blocks in the system are quantizer, bit allocation, buffer, multiplexer, channel coding etc.

A gallium-arsenide digital phase shifter for clock and control signal distribution in high-speed digital systems

NASA Technical Reports Server (NTRS)

Fouts, Douglas J.

1992-01-01

The design, implementation, testing, and applications of a gallium-arsenide digital phase shifter and fan-out buffer are described. The integrated circuit provides a method for adjusting the phase of high-speed clock and control signals in digital systems, without the need for pruning cables, multiplexing between cables of different lengths, delay lines, or similar techniques. The phase of signals distributed with the described chip can be dynamically adjusted in eight different steps of approximately 60 ps per step. The IC also serves as a fan-out buffer and provides 12 in-phase outputs. The chip is useful for distributing high-speed clock and control signals in synchronous digital systems, especially if components are distributed over a large physical area or if there is a large number of components.

Power-efficient method for IM-DD optical transmission of multiple OFDM signals.

PubMed

Effenberger, Frank; Liu, Xiang

2015-05-18

We propose a power-efficient method for transmitting multiple frequency-division multiplexed (FDM) orthogonal frequency-division multiplexing (OFDM) signals in intensity-modulation direct-detection (IM-DD) optical systems. This method is based on quadratic soft clipping in combination with odd-only channel mapping. We show, both analytically and experimentally, that the proposed approach is capable of improving the power efficiency by about 3 dB as compared to conventional FDM OFDM signals under practical bias conditions, making it a viable solution in applications such as optical fiber-wireless integrated systems where both IM-DD optical transmission and OFDM signaling are important.

Decision-feedback detection strategy for nonlinear frequency-division multiplexing

NASA Astrophysics Data System (ADS)

Civelli, Stella; Forestieri, Enrico; Secondini, Marco

2018-04-01

By exploiting a causality property of the nonlinear Fourier transform, a novel decision-feedback detection strategy for nonlinear frequency-division multiplexing (NFDM) systems is introduced. The performance of the proposed strategy is investigated both by simulations and by theoretical bounds and approximations, showing that it achieves a considerable performance improvement compared to previously adopted techniques in terms of Q-factor. The obtained improvement demonstrates that, by tailoring the detection strategy to the peculiar properties of the nonlinear Fourier transform, it is possible to boost the performance of NFDM systems and overcome current limitations imposed by the use of more conventional detection techniques suitable for the linear regime.

Self-homodyne optical OFDM for broadband WDM-PONs with crosstalk-free remodulation and enhanced tolerance to Rayleigh noise

NASA Astrophysics Data System (ADS)

Lyu, WeiChao; Wang, Andong; Xie, Dequan; Zhu, Long; Guan, Xun; Wang, Jian; Xu, Jing

2018-05-01

We propose a novel architecture for wavelength-division-multiplexed passive optical network (WDM-PON) that can simultaneously circumvent both remodulation crosstalk and Rayleigh noise, based on self-homodyne detection and optical orthogonal frequency-division multiplexing (OFDM) remodulation. The proposed self-homodyne detection at optical network unit (ONU) requires neither frequency offset compensation nor phase noise compensation, and thus can significantly reduce system complexity and power consumption. Bidirectional transmission of 12.5 Gb/s down- and up-stream signals, via single 25 km single-mode fiber without dispersion compensation, is demonstrated in a proof-of-concept experiment.

Design, Fabrication, and Testing of Lumped Element Kinetic inductance Detectors for 3 mm CMB Observations

NASA Technical Reports Server (NTRS)

Lowitz, Amy E.; Brown, Ari David; Stevenson, Thomas R.; Timbie, Peter T.; Wollack, Edward J.

2014-01-01

Kinetic inductance detectors (KIDs) are a promising technology for low-noise, highly-multiplexible mm- and submm-wave detection. KIDs have a number of advantages over other detector technologies, which make them an appealing option in the cosmic microwave background B-mode anisotropy search, including passive frequency domain multiplexing and relatively simple fabrication, but have suffered from challenges associated with noise control. Here we describe design and fabrication of a 20-pixel prototype array of lumped element molybdenum KIDs. We show Q, frequency and temperature measurements from the array under dark conditions. We also present evidence for a double superconducting gap in molybdenum.

High-speed bioimaging with frequency-division-multiplexed fluorescence confocal microscopy

NASA Astrophysics Data System (ADS)

Mikami, Hideharu; Harmon, Jeffrey; Ozeki, Yasuyuki; Goda, Keisuke

2017-04-01

We present methods of fluorescence confocal microscopy that enable unprecedentedly high frame rate of > 10,000 fps. The methods are based on a frequency-division multiplexing technique, which was originally developed in the field of communication engineering. Specifically, we achieved a broad bandwidth ( 400 MHz) of detection signals using a dual- AOD method and overcame limitations in frame rate, due to a scanning device, by using a multi-line focusing method, resulting in a significant increase in frame rate. The methods have potential biomedical applications such as observation of sub-millisecond dynamics in biological tissues, in-vivo three-dimensional imaging, and fluorescence imaging flow cytometry.

An IEEE802.15.4-Based System for Locating Children on Their School Commutes

NASA Astrophysics Data System (ADS)

Sugiura, Akihiko; Baba, Ryoichi; Kobayashi, Hideyuki

With the increasing number of crimes and accidents in which children are becoming involved, there is a growing demand for devices to safeguard children's security by detecting their locations on their way to and from school. This paper proposes a system that uses an IEEE802.15.4-standard network to detect children's locations. To overcome the susceptibility of radio interference from nearby wireless LANs, frequency division multiplexing is applied to this IEEE802.15.4-based network, toward improving data acquisition from terminal units. The effectiveness of the system was field-tested with elementary school students who used about 400 IEEE 802.15.4-compliant terminal units. An experiment verified that the use of frequency division multiplexing in an environment where radio interference by wireless LANs is strong allowed the network to double the success rate of information communication from terminal units relative to that without frequency division multiplexing. In the experiment for detecting elementary schoolers' arrival at and departure from school, the terminal detection rate was 99% and the terminal detection rate on the designated school routes was 90%. These results prove the effectiveness of the system in detecting locations.

Frequency-division multiplexer and demultiplexer for terahertz wireless links.

PubMed

Ma, Jianjun; Karl, Nicholas J; Bretin, Sara; Ducournau, Guillaume; Mittleman, Daniel M

2017-09-28

The development of components for terahertz wireless communications networks has become an active and growing research field. However, in most cases these components have been studied using a continuous or broadband-pulsed terahertz source, not using a modulated data stream. This limitation may mask important aspects of the performance of the device in a realistic system configuration. We report the characterization of one such device, a frequency multiplexer, using modulated data at rates up to 10 gigabits per second. We also demonstrate simultaneous error-free transmission of two signals at different carrier frequencies, with an aggregate data rate of 50 gigabits per second. We observe that the far-field spatial variation of the bit error rate is different from that of the emitted power, due to a small nonuniformity in the angular detection sensitivity. This is likely to be a common feature of any terahertz communication system in which signals propagate as diffracting beams not omnidirectional broadcasts.There is growing interest in the development of components to facilitate wireless communications in the terahertz but the characterization of these systems involve an unmodulated input. Here the authors demonstrate multiplexing and demultiplexing of data streams in the terahertz range using a real data link.

Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.

PubMed

Qi, Zongtai; Ma, Yinjiao; Deng, Lili; Wu, Haiping; Zhou, Guohua; Kajiyama, Tomoharu; Kambara, Hideki

2011-06-07

To digitally analyze expression levels of multiple genes in one reaction, we proposed a method termed as 'MDHB' (Multiplexed Digital-PCR coupled with Hydrogel Bead-array). The template for bead-based emulsion PCR (emPCR) was prepared by reverse transcription using sequence-tagged primers. The beads recovered from emPCR were immobilized with hydrogel to form a single-bead layer on a chip, and then decoded by gene-specific probe hybridization and Cy3-dUTP based primer extension reaction. The specificity of probe hybridization was improved by using electrophoresis to remove mismatched probes on the bead's surface. The number of positive beads reflects the abundance of expressed genes; the expression levels of target genes were normalized to a housekeeping gene and expressed as the number ratio of green beads to red beads. The discrimination limit of MDHB is 0.1% (i.e., one target molecule from 1000 background molecules), and the sensitivity of the method is below 100 cells when using the β-actin gene as the detection target. We have successfully employed MDHB to detect the relative expression levels of four colorectal cancer (CRC)-related genes (c-myc, COX-2, MMP7, and DPEP1) in 8 tissue samples and 9 stool samples from CRC patients, giving the detection rates of 100% and 77%, respectively. The results suggest that MDHB could be a potential tool for early non-invasive diagnosis of CRC.

Terabit optical OFDM superchannel transmission via coherent carriers of a hybrid chip-scale soliton frequency comb

NASA Astrophysics Data System (ADS)

Geng, Yong; Huang, Xiatao; Cui, Wenwen; Ling, Yun; Xu, Bo; Zhang, Jin; Yi, Xingwen; Wu, Baojian; Huang, Shu-Wei; Qiu, Kun; Wong, Chee Wei; Zhou, Heng

2018-05-01

We demonstrate seamless channel multiplexing and high bitrate superchannel transmission of coherent optical orthogonal-frequency-division-multiplexing (CO-OFDM) data signals utilizing a dissipative Kerr soliton (DKS) frequency comb generated in an on-chip microcavity. Aided by comb line multiplication through Nyquist pulse modulation, the high stability and mutual coherence among mode-locked Kerr comb lines are exploited for the first time to eliminate the guard intervals between communication channels and achieve full spectral density bandwidth utilization. Spectral efficiency as high as 2.625 bit/Hz/s is obtained for 180 CO-OFDM bands encoded with 12.75 Gbaud 8-QAM data, adding up to total bitrate of 6.885 Tb/s within 2.295 THz frequency comb bandwidth. Our study confirms that high coherence is the key superiority of Kerr soliton frequency combs over independent laser diodes, as a multi-spectral coherent laser source for high-bandwidth high-spectral-density transmission networks.

Destination directed packet switch architecture for a 30/20 GHz FDMA/TDM geostationary communication satellite network

NASA Technical Reports Server (NTRS)

Ivancic, William D.; Shalkhauser, Mary JO

1991-01-01

Emphasis is on a destination directed packet switching architecture for a 30/20 GHz frequency division multiplex access/time division multiplex (FDMA/TDM) geostationary satellite communication network. Critical subsystems and problem areas are identified and addressed. Efforts have concentrated heavily on the space segment; however, the ground segment was considered concurrently to ensure cost efficiency and realistic operational constraints.

High-resolution photon spectroscopy with a microwave-multiplexed 4-pixel transition edge sensor array

NASA Astrophysics Data System (ADS)

Guss, Paul; Rabin, Michael; Croce, Mark; Hoteling, Nathan; Schwellenbach, David; Kruschwitz, Craig; Mocko, Veronika; Mukhopadhyay, Sanjoy

2017-09-01

We demonstrate very high-resolution photon spectroscopy with a microwave-multiplexed 4-pixel transition edge sensor (TES) array. The readout circuit consists of superconducting microwave resonators coupled to radio frequency superconducting-quantum-interference devices (RF-SQUIDs) and transduces changes in input current to changes in phase of a microwave signal. We used a flux-ramp modulation to linearize the response and avoid low-frequency noise. The result is a very high-resolution photon spectroscopy with a microwave-multiplexed 4-pixel transition edge sensor array. We performed and validated a small-scale demonstration and test of all the components of our concept system, which encompassed microcalorimetry, microwave multiplexing, RF-SQUIDs, and software-defined radio (SDR). We shall display data we acquired in the first simultaneous combination of all key innovations in a 4-pixel demonstration, including microcalorimetry, microwave multiplexing, RF-SQUIDs, and SDR. We present the energy spectrum of a gadolinium-153 (153Gd) source we measured using our 4-pixel TES array and the RF-SQUID multiplexer. For each pixel, one can observe the two 97.4 and 103.2 keV photopeaks. We measured the 153Gd photon source with an achieved energy resolution of 70 eV, full width half maximum (FWHM) at 100 keV, and an equivalent readout system noise of 90 pA/pHz at the TES. This demonstration establishes a path for the readout of cryogenic x-ray and gamma ray sensor arrays with more elements and spectral resolving powers. We believe this project has improved capabilities and substantively advanced the science useful for missions such as nuclear forensics, emergency response, and treaty verification through the explored TES developments.

Three-Level De-Multiplexed Dual-Branch Complex Delta-Sigma Transmitter.

PubMed

Arfi, Anis Ben; Elsayed, Fahmi; Aflaki, Pouya M; Morris, Brad; Ghannouchi, Fadhel M

2018-02-20

In this paper, a dual-branch topology driven by a Delta-Sigma Modulator (DSM) with a complex quantizer, also known as the Complex Delta Sigma Modulator (CxDSM), with a 3-level quantized output signal is proposed. By de-multiplexing the 3-level Delta-Sigma-quantized signal into two bi-level streams, an efficiency enhancement over the operational frequency range is achieved. The de-multiplexed signals drive a dual-branch amplification block composed of two switch-mode back-to-back power amplifiers working at peak power. A signal processing technique known as quantization noise reduction with In-band Filtering (QNRIF) is applied to each of the de-multiplexed streams to boost the overall performances; particularly the Adjacent Channel Leakage Ratio (ACLR). After amplification, the two branches are combined using a non-isolated combiner, preserving the efficiency of the transmitter. A comprehensive study on the operation of this topology and signal characteristics used to drive the dual-branch Switch-Mode Power Amplifiers (SMPAs) was established. Moreover, this work proposes a highly efficient design of the amplification block based on a back-to-back power topology performing a dynamic load modulation exploiting the non-overlapping properties of the de-multiplexed Complex DSM signal. For experimental validation, the proposed de-multiplexed 3-level Delta-Sigma topology was implemented on the BEEcube™ platform followed by the back-to-back Class-E switch-mode power amplification block. The full transceiver is assessed using a 4th-Generation mobile communications standard LTE (Long Term Evolution) standard 1.4 MHz signal with a peak to average power ratio (PAPR) of 8 dB. The dual-branch topology exhibited a good linearity and a coding efficiency of the transmitter chain higher than 72% across the band of frequency from 1.8 GHz to 2.7 GHz.

Evaluation of Two Highly-Multiplexed Custom Panels for Massively Parallel Semiconductor Sequencing on Paraffin DNA

PubMed Central

Kotoula, Vassiliki; Lyberopoulou, Aggeliki; Papadopoulou, Kyriaki; Charalambous, Elpida; Alexopoulou, Zoi; Gakou, Chryssa; Lakis, Sotiris; Tsolaki, Eleftheria; Lilakos, Konstantinos; Fountzilas, George

2015-01-01

Background—Aim Massively parallel sequencing (MPS) holds promise for expanding cancer translational research and diagnostics. As yet, it has been applied on paraffin DNA (FFPE) with commercially available highly multiplexed gene panels (100s of DNA targets), while custom panels of low multiplexing are used for re-sequencing. Here, we evaluated the performance of two highly multiplexed custom panels on FFPE DNA. Methods Two custom multiplex amplification panels (B, 373 amplicons; T, 286 amplicons) were coupled with semiconductor sequencing on DNA samples from FFPE breast tumors and matched peripheral blood samples (n samples: 316; n libraries: 332). The two panels shared 37% DNA targets (common or shifted amplicons). Panel performance was evaluated in paired sample groups and quartets of libraries, where possible. Results Amplicon read ratios yielded similar patterns per gene with the same panel in FFPE and blood samples; however, performance of common amplicons differed between panels (p<0.001). FFPE genotypes were compared for 1267 coding and non-coding variant replicates, 999 out of which (78.8%) were concordant in different paired sample combinations. Variant frequency was highly reproducible (Spearman’s rho 0.959). Repeatedly discordant variants were of high coverage / low frequency (p<0.001). Genotype concordance was (a) high, for intra-run duplicates with the same panel (mean±SD: 97.2±4.7, 95%CI: 94.8–99.7, p<0.001); (b) modest, when the same DNA was analyzed with different panels (mean±SD: 81.1±20.3, 95%CI: 66.1–95.1, p = 0.004); and (c) low, when different DNA samples from the same tumor were compared with the same panel (mean±SD: 59.9±24.0; 95%CI: 43.3–76.5; p = 0.282). Low coverage / low frequency variants were validated with Sanger sequencing even in samples with unfavourable DNA quality. Conclusions Custom MPS may yield novel information on genomic alterations, provided that data evaluation is adjusted to tumor tissue FFPE DNA. To this scope, eligibility of all amplicons along with variant coverage and frequency need to be assessed. PMID:26039550

Forensic and population genetic analyses of eighteen non-CODIS miniSTR loci in the Korean population.

PubMed

Jin, Han Jun; Kim, Ki Cheol; Yoon, Cha Eun; Kim, Wook

2013-11-01

We analyzed the variation of eighteen miniSTR loci in 411 randomly chosen individuals from Korea to increase the probability that a degraded sample can be typed, as well as to provide an expanded and reliable population database. Six multiplex PCR systems were developed (multiplex I: D1S1677, D2S441 and D4S2364; multiplex II: D10S1248, D14S1434 and D22S1045; multiplex III: D12S391, D16S3253 and D20S161; multiplex IV: D3S4529, D8S1115 and D18S853; multiplex V: D6S1017, D11S4463 and D17S1301; multiplex VI: D5S2500, D9S1122 and D21S1437). Allele frequencies and forensic parameters were calculated to evaluate the suitability and robustness of these non-CODIS miniSTR systems. No significant deviation from Hardy-Weinberg equilibrium expectations were observed, except for D4S2364, D5S2500 and D20S161 loci. A multidimensional scaling plot based on allele frequencies of the six miniSTR loci (D1S1677, D2S441, D4S2364, D10S1248, D14S1434 and D22S1045) showed that Koreans appeared to have most genetic affinity with Chinese and Japanese than to other Eurasian populations compared here. The combined probability of match calculated from the 18 miniSTR loci was 2.902 × 10(-17), indicating a high degree of polymorphism. Thus, the 18 miniSTR loci can be suitable for recovering useful information for analyzing degraded forensic casework samples and for adding supplementary genetic information for a variety of analyses involving closely related individuals where there is a need for additional genetic information. Copyright © 2013 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

Automatic Modulation Classification of Common Communication and Pulse Compression Radar Waveforms using Cyclic Features

DTIC Science & Technology

2013-03-01

intermediate frequency LFM linear frequency modulation MAP maximum a posteriori MATLAB® matrix laboratory ML maximun likelihood OFDM orthogonal frequency...spectrum, frequency hopping, and orthogonal frequency division multiplexing ( OFDM ) modulations. Feature analysis would be a good research thrust to...determine feature relevance and decide if removing any features improves performance. Also, extending the system for simulations using a MIMO receiver or

Compact pulse width modulation circuitry for silicon photomultiplier readout.

PubMed

Bieniosek, M F; Olcott, P D; Levin, C S

2013-08-07

The adoption of solid-state photodetectors for positron emission tomography (PET) system design and the interest in 3D interaction information from PET detectors has lead to an increasing number of readout channels in PET systems. To handle these additional readout channels, PET readout electronics should be simplified to reduce the power consumption, cost, and size of the electronics for a single channel. Pulse-width modulation (PWM), where detector pulses are converted to digital pulses with width proportional to the detected photon energy, promises to simplify PET readout by converting the signals to digital form at the beginning of the processing chain, and allowing a single time-to-digital converter to perform the data acquisition for many channels rather than routing many analogue channels and digitizing in the back end. Integrator based PWM systems, also known as charge-to-time converters (QTCs), are especially compact, reducing the front-end electronics to an op-amp integrator with a resistor discharge, and a comparator. QTCs, however, have a long dead-time during which dark count noise is integrated, reducing the output signal-to-noise ratio. This work presents a QTC based PWM circuit with a gated integrator that shows performance improvements over existing QTC based PWM. By opening and closing an analogue switch on the input of the integrator, the circuit can be controlled to integrate only the portions of the signal with a high signal-to-noise ratio. It also allows for multiplexing different detectors into the same PWM circuit while avoiding uncorrelated noise propagation between photodetector channels. Four gated integrator PWM circuits were built to readout the spatial channels of two position sensitive solid-state photomultiplier (PS-SSPM). Results show a 4 × 4 array 0.9 mm × 0.9 mm × 15 mm of LYSO crystals being identified on the 5 mm × 5 mm PS-SSPM at room temperature with no degradation for twofold multiplexing. In principle, much larger multiplexing ratios are possible, limited only by count rate issues.

Effect of Shot Noise on Simultaneous Sensing in Frequency Division Multiplexed Diffuse Optical Tomographic Imaging Process.

PubMed

Jang, Hansol; Lim, Gukbin; Hong, Keum-Shik; Cho, Jaedu; Gulsen, Gultekin; Kim, Chang-Seok

2017-11-28

Diffuse optical tomography (DOT) has been studied for use in the detection of breast cancer, cerebral oxygenation, and cognitive brain signals. As optical imaging studies have increased significantly, acquiring imaging data in real time has become increasingly important. We have developed frequency-division multiplexing (FDM) DOT systems to analyze their performance with respect to acquisition time and imaging quality, in comparison with the conventional time-division multiplexing (TDM) DOT. A large tomographic area of a cylindrical phantom 60 mm in diameter could be successfully reconstructed using both TDM DOT and FDM DOT systems. In our experiment with 6 source-detector (S-D) pairs, the TDM DOT and FDM DOT systems required 6.18 and 1 s, respectively, to obtain a single tomographic data set. While the absorption coefficient of the reconstruction image was underestimated in the case of the FDM DOT, we experimentally confirmed that the abnormal region can be clearly distinguished from the background phantom using both methods.

Low-peak-to-average power ratio and low-complexity asymmetrically clipped optical orthogonal frequency-division multiplexing uplink transmission scheme for long-reach passive optical network.

PubMed

Zhou, Ji; Qiao, Yaojun

2015-09-01

In this Letter, we propose a discrete Hartley transform (DHT)-spread asymmetrically clipped optical orthogonal frequency-division multiplexing (DHT-S-ACO-OFDM) uplink transmission scheme in which the multiplexing/demultiplexing process also uses the DHT algorithm. By designing a simple encoding structure, the computational complexity of the transmitter can be reduced from O(Nlog(2)(N)) to O(N). At the probability of 10(-3), the peak-to-average power ratio (PAPR) of 2-ary pulse amplitude modulation (2-PAM)-modulated DHT-S-ACO-OFDM is approximately 9.7 dB lower than that of 2-PAM-modulated conventional ACO-OFDM. To verify the feasibility of the proposed scheme, a 4-Gbit/s DHT-S-ACO-OFDM uplink transmission scheme with a 1∶64 way split has been experimentally implemented using 100-km standard single-mode fiber (SSMF) for a long-reach passive optical network (LR-PON).

A universal array-based multiplexed test for cystic fibrosis carrier screening.

PubMed

Amos, Jean A; Bridge-Cook, Philippa; Ponek, Victor; Jarvis, Michael R

2006-01-01

Cystic fibrosis is a multisystem autosomal recessive disorder with high carrier frequencies in caucasians and significant, but lower, carrier frequencies in other ethnicities. Based on technology that allows high detection of mutations in caucasians and significant detection in other ethnic groups, the American College of Medical Genetics (ACMG) and American College of Obstetricians and Gynecologists (ACOG) have recommended pan-ethnic cystic fibrosis carrier screening for all reproductive couples. This paper discusses carrier screening using the Tag-It multiplex mutation platform and the Cystic Fibrosis Mutation Detection Kit. The Tag-It cystic fibrosis assay is a multiplexed genotyping assay that detects a panel of 40 cystic fibrosis transmembrane conductance regulator mutations including the 23 mutations recommended by the ACMG and ACOG for population screening. A total of 16 additional mutations detected by the Tag-It cystic fibrosis assay may also be common. The assay method is described in detail, and its performance in a genetics reference laboratory performing high-volume cystic fibrosis carrier screening is assessed.

Optical frequency comb generation with high tone-to-noise ratio for large-capacity wavelength division multiplexed passive optical network

NASA Astrophysics Data System (ADS)

Ullah, Rahat; Liu, Bo; Zhang, Qi; Tian, Qinghua; Tian, Feng; Qu, Zhaowei; Yan, Cheng; Khan, Muhammad Saad; Ahmad, Ibrar; Xin, Xiangjun

2015-11-01

We propose a technique for the generation of optical frequency comb from a single source, which reduces the costs of optical access networks. Two Mach-Zehnder modulators are cascaded with one phase modulator driven by radiofrequency signals. With 10-GHz frequency spacing, the generated 40 optical multicarriers have good tone-to-noise ratio with least excursions in their comb lines. The laser array at the optical line terminal of the conventional wavelength division multiplexed passive optical network (WDM-PON) system has been replaced with optical frequency comb generator (OFCG), which may result in cost-effective optical line terminal (OLT) supporting a large-capacity WDM-PON system. Of 40 carriers generated, each carrier carries 10 Gbps data based on differential phase-shift keying. Four hundred Gbps multiplexed data from all channels are successfully transmitted through a fiber span of 25 km with negligible power penalties. Part of the downlink signal is used in uplink transmission at optical network unit where intensity-modulated on-off keying is deployed for remodulation. Theoretical analysis of the proposed WDM-PON system based on OFCG are in good agreement with simulation results. The metrics considered for the analysis of the proposed OFCG in a WDM-PON system are power penalties of the full-duplex transmission, eye diagrams, and bit error rate.

Custom instruction set NIOS-based OFDM processor for FPGAs

NASA Astrophysics Data System (ADS)

Meyer-Bäse, Uwe; Sunkara, Divya; Castillo, Encarnacion; Garcia, Antonio

2006-05-01

Orthogonal Frequency division multiplexing (OFDM) spread spectrum technique, sometimes also called multi-carrier or discrete multi-tone modulation, are used in bandwidth-efficient communication systems in the presence of channel distortion. The benefits of OFDM are high spectral efficiency, resiliency to RF interference, and lower multi-path distortion. OFDM is the basis for the European digital audio broadcasting (DAB) standard, the global asymmetric digital subscriber line (ADSL) standard, in the IEEE 802.11 5.8 GHz band standard, and ongoing development in wireless local area networks. The modulator and demodulator in an OFDM system can be implemented by use of a parallel bank of filters based on the discrete Fourier transform (DFT), in case the number of subchannels is large (e.g. K > 25), the OFDM system are efficiently implemented by use of the fast Fourier transform (FFT) to compute the DFT. We have developed a custom FPGA-based Altera NIOS system to increase the performance, programmability, and low power in mobil wireless systems. The overall gain observed for a 1024-point FFT ranges depending on the multiplier used by the NIOS processor between a factor of 3 and 16. A careful optimization described in the appendix yield a performance gain of up to 77% when compared with our preliminary results.

Ultra-dense WDM-PON delivering carrier-centralized Nyquist-WDM uplink with digital coherent detection.

PubMed

Dong, Ze; Yu, Jianjun; Chien, Hung-Chang; Chi, Nan; Chen, Lin; Chang, Gee-Kung

2011-06-06

We introduce an "ultra-dense" concept into next-generation WDM-PON systems, which transmits a Nyquist-WDM uplink with centralized uplink optical carriers and digital coherent detection for the future access network requiring both high capacity and high spectral efficiency. 80-km standard single mode fiber (SSMF) transmission of Nyquist-WDM signal with 13 coherent 25-GHz spaced wavelength shaped optical carriers individually carrying 100-Gbit/s polarization-multiplexing quadrature phase-shift keying (PM-QPSK) upstream data has been experimentally demonstrated with negligible transmission penalty. The 13 frequency-locked wavelengths with a uniform optical power level of -10 dBm and OSNR of more than 50 dB are generated from a single lightwave via a multi-carrier generator consists of an optical phase modulator (PM), a Mach-Zehnder modulator (MZM), and a WSS. Following spacing the carriers at the baud rate, sub-carriers are individually spectral shaped to form Nyquist-WDM. The Nyquist-WDM channels have less than 1-dB crosstalk penalty of optical signal-to-noise ratio (OSNR) at 2 × 10(-3) bit-error rate (BER). Performance of a traditional coherent optical OFDM scheme and its restrictions on symbol synchronization and power difference are also experimentally compared and studied.

Multiplexed operation of a micromachined ultrasonic droplet ejector array.

PubMed

Forbes, Thomas P; Degertekin, F Levent; Fedorov, Andrei G

2007-10-01

A dual-sample ultrasonic droplet ejector array is developed for use as a soft-ionization ion source for multiplexed mass spectrometry (MS). Such a multiplexed ion source aims to reduce MS analysis time for multiple analyte streams, as well as allow for the synchronized ejection of the sample(s) and an internal standard for quantitative results and mass calibration. Multiplexing is achieved at the device level by division of the fluid reservoir and separating the active electrodes of the piezoelectric transducer for isolated application of ultrasonic wave energy to each domain. The transducer is mechanically shaped to further reduce the acoustical crosstalk between the domains. Device design is performed using finite-element analysis simulations and supported by experimental characterization. Isolated ejection of approximately 5 microm diameter water droplets from individual domains in the micromachined droplet ejector array at around 1 MHz frequency is demonstrated by experiments. The proof-of-concept demonstration using a dual-sample device also shows potential for multiplexing with larger numbers of analytes.

Multiplexed operation of a micromachined ultrasonic droplet ejector array

DOE Office of Scientific and Technical Information (OSTI.GOV)

Forbes, Thomas P.; Degertekin, F. Levent; Fedorov, Andrei G.

2007-10-15

A dual-sample ultrasonic droplet ejector array is developed for use as a soft-ionization ion source for multiplexed mass spectrometry (MS). Such a multiplexed ion source aims to reduce MS analysis time for multiple analyte streams, as well as allow for the synchronized ejection of the sample(s) and an internal standard for quantitative results and mass calibration. Multiplexing is achieved at the device level by division of the fluid reservoir and separating the active electrodes of the piezoelectric transducer for isolated application of ultrasonic wave energy to each domain. The transducer is mechanically shaped to further reduce the acoustical crosstalk betweenmore » the domains. Device design is performed using finite-element analysis simulations and supported by experimental characterization. Isolated ejection of {approx}5 {mu}m diameter water droplets from individual domains in the micromachined droplet ejector array at around 1 MHz frequency is demonstrated by experiments. The proof-of-concept demonstration using a dual-sample device also shows potential for multiplexing with larger numbers of analytes.« less

Development of a Digital Microarray with Interferometric Reflectance Imaging

NASA Astrophysics Data System (ADS)

Sevenler, Derin

This dissertation describes a new type of molecular assay for nucleic acids and proteins. We call this technique a digital microarray since it is conceptually similar to conventional fluorescence microarrays, yet it performs enumerative ('digital') counting of the number captured molecules. Digital microarrays are approximately 10,000-fold more sensitive than fluorescence microarrays, yet maintain all of the strengths of the platform including low cost and high multiplexing (i.e., many different tests on the same sample simultaneously). Digital microarrays use gold nanorods to label the captured target molecules. Each gold nanorod on the array is individually detected based on its light scattering, with an interferometric microscopy technique called SP-IRIS. Our optimized high-throughput version of SP-IRIS is able to scan a typical array of 500 spots in less than 10 minutes. Digital DNA microarrays may have utility in applications where sequencing is prohibitively expensive or slow. As an example, we describe a digital microarray assay for gene expression markers of bacterial drug resistance.

Reproducibility of Digital PCR Assays for Circulating Tumor DNA Analysis in Advanced Breast Cancer

PubMed Central

Hrebien, Sarah; O’Leary, Ben; Beaney, Matthew; Schiavon, Gaia; Fribbens, Charlotte; Bhambra, Amarjit; Johnson, Richard; Turner, Nicholas

2016-01-01

Circulating tumor DNA (ctDNA) analysis has the potential to allow non-invasive analysis of tumor mutations in advanced cancer. In this study we assessed the reproducibility of digital PCR (dPCR) assays of circulating tumor DNA in a cohort of patients with advanced breast cancer and assessed delayed plasma processing using cell free DNA preservative tubes. We recruited a cohort of 96 paired samples from 71 women with advanced breast cancer who had paired blood samples processed either immediately or delayed in preservative tubes with processing 48–72 hours after collection. Plasma DNA was analysed with multiplex digital PCR (mdPCR) assays for hotspot mutations in PIK3CA, ESR1 and ERBB2, and for AKT1 E17K. There was 94.8% (91/96) agreement in mutation calling between immediate and delayed processed tubes, kappa 0.88 95% CI 0.77–0.98). Discordance in mutation calling resulted from low allele frequency and likely stochastic effects. In concordant samples there was high correlation in mutant copies per ml plasma (r2 = 0.98; p<0.0001). There was elevation of total cell free plasma DNA concentrations in 10.3% of delayed processed tubes, although overall quantification of total cell free plasma DNA had similar prognostic effects in immediate (HR 3.6) and delayed (HR 3.0) tubes. There was moderate agreement in changes in allele fraction between sequential samples in quantitative mutation tracking (r = 0.84, p = 0.0002). Delayed processing of samples using preservative tubes allows for centralized ctDNA digital PCR mutation screening in advanced breast cancer. The potential of preservative tubes in quantitative mutation tracking requires further research. PMID:27760227

Reproducibility of Digital PCR Assays for Circulating Tumor DNA Analysis in Advanced Breast Cancer.

PubMed

Hrebien, Sarah; O'Leary, Ben; Beaney, Matthew; Schiavon, Gaia; Fribbens, Charlotte; Bhambra, Amarjit; Johnson, Richard; Garcia-Murillas, Isaac; Turner, Nicholas

2016-01-01

Circulating tumor DNA (ctDNA) analysis has the potential to allow non-invasive analysis of tumor mutations in advanced cancer. In this study we assessed the reproducibility of digital PCR (dPCR) assays of circulating tumor DNA in a cohort of patients with advanced breast cancer and assessed delayed plasma processing using cell free DNA preservative tubes. We recruited a cohort of 96 paired samples from 71 women with advanced breast cancer who had paired blood samples processed either immediately or delayed in preservative tubes with processing 48-72 hours after collection. Plasma DNA was analysed with multiplex digital PCR (mdPCR) assays for hotspot mutations in PIK3CA, ESR1 and ERBB2, and for AKT1 E17K. There was 94.8% (91/96) agreement in mutation calling between immediate and delayed processed tubes, kappa 0.88 95% CI 0.77-0.98). Discordance in mutation calling resulted from low allele frequency and likely stochastic effects. In concordant samples there was high correlation in mutant copies per ml plasma (r2 = 0.98; p<0.0001). There was elevation of total cell free plasma DNA concentrations in 10.3% of delayed processed tubes, although overall quantification of total cell free plasma DNA had similar prognostic effects in immediate (HR 3.6) and delayed (HR 3.0) tubes. There was moderate agreement in changes in allele fraction between sequential samples in quantitative mutation tracking (r = 0.84, p = 0.0002). Delayed processing of samples using preservative tubes allows for centralized ctDNA digital PCR mutation screening in advanced breast cancer. The potential of preservative tubes in quantitative mutation tracking requires further research.

A polarization-division multiplexing SSB-OFDM system with beat interference cancellation receivers

NASA Astrophysics Data System (ADS)

Yang, Peiling; Ma, Jianxin; Zhang, Junyi

2018-06-01

In this paper, we have proposed a polarization-division multiplexing (PDM) single-sideband optical orthogonal frequency division multiplexing (SSB-OOFDM) scheme with signal-signal beat interference cancellation receivers with balanced detection (ICRBD). This system can double channel capacity and improve spectrum efficiency (SE) with the reduced guard band (GB) due to the PDM. Multiple input multiple output (MIMO) technique is used to solve polarization mode dispersion (PMD) associated with channel estimation and equalization. By simulation, we demonstrate the efficacy of the proposed technique for a 2 ×40 Gbit/s 16-QAM SSB-PDM-OOFDM system according to the error vector magnitude (EVM) and the constellation diagrams.

Apollo experience report: Real-time display system

NASA Technical Reports Server (NTRS)

Sullivan, C. J.; Burbank, L. W.

1976-01-01

The real time display system used in the Apollo Program is described; the systematic organization of the system, which resulted from hardware/software trade-offs and the establishment of system criteria, is emphasized. Each basic requirement of the real time display system was met by a separate subsystem. The computer input multiplexer subsystem, the plotting display subsystem, the digital display subsystem, and the digital television subsystem are described. Also described are the automated display design and the generation of precision photographic reference slides required for the three display subsystems.

Digital system bus integrity

NASA Technical Reports Server (NTRS)

Eldredge, Donald; Hitt, Ellis F.

1987-01-01

This report summarizes and describes the results of a study of current or emerging multiplex data buses as applicable to digital flight systems, particularly with regard to civil aircraft. Technology for pre-1995 and post-1995 timeframes has been delineated and critiqued relative to the requirements envisioned for those periods. The primary emphasis has been an assured airworthiness of the more prevalent type buses, with attention to attributes such as fault tolerance, environmental susceptibility, and problems under continuing investigation. Additionally, the capacity to certify systems relying on such buses has been addressed.

Optical information processing at NASA Ames Research Center

NASA Technical Reports Server (NTRS)

Reid, Max B.; Bualat, Maria G.; Cho, Young C.; Downie, John D.; Gary, Charles K.; Ma, Paul W.; Ozcan, Meric; Pryor, Anna H.; Spirkovska, Lilly

1993-01-01

The combination of analog optical processors with digital electronic systems offers the potential of tera-OPS computational performance, while often requiring less power and weight relative to all-digital systems. NASA is working to develop and demonstrate optical processing techniques for on-board, real time science and mission applications. Current research areas and applications under investigation include optical matrix processing for space structure vibration control and the analysis of Space Shuttle Main Engine plume spectra, optical correlation-based autonomous vision for robotic vehicles, analog computation for robotic path planning, free-space optical interconnections for information transfer within digital electronic computers, and multiplexed arrays of fiber optic interferometric sensors for acoustic and vibration measurements.

Independent Orbiter Assessment (IOA): Analysis of the instrumentation subsystem

NASA Technical Reports Server (NTRS)

Howard, B. S.

1986-01-01

The results of the Independent Orbiter Assessment (IOA) of the Failure Modes and Effects Analysis (FMEA) and Critical Items List (CIL) are presented. The IOA approach features a top-down analysis of the hardware to determine failure modes, criticality, and potential critical items. To preserve independence, this analysis was accomplished without reliance upon the results contained within the NASA FMEA/CIL documentation. The independent analysis results for the Instrumentation Subsystem are documented. The Instrumentation Subsystem (SS) consists of transducers, signal conditioning equipment, pulse code modulation (PCM) encoding equipment, tape recorders, frequency division multiplexers, and timing equipment. For this analysis, the SS is broken into two major groupings: Operational Instrumentation (OI) equipment and Modular Auxiliary Data System (MADS) equipment. The OI equipment is required to acquire, condition, scale, digitize, interleave/multiplex, format, and distribute operational Orbiter and payload data and voice for display, recording, telemetry, and checkout. It also must provide accurate timing for time critical functions for crew and payload specialist use. The MADS provides additional instrumentation to measure and record selected pressure, temperature, strain, vibration, and event data for post-flight playback and analysis. MADS data is used to assess vehicle responses to the flight environment and to permit correlation of such data from flight to flight. The IOA analysis utilized available SS hardware drawings and schematics for identifying hardware assemblies and components and their interfaces. Criticality for each item was assigned on the basis of the worst-case effect of the failure modes identified.

Low-to-Medium Power Single Chip Digital Controlled DC-DC Regulator for Point-of-Load Applications

NASA Technical Reports Server (NTRS)

Adell, Philippe C. (Inventor); Bakkaloglu, Bertan (Inventor); Vermeire, Bert (Inventor); Liu, Tao (Inventor)

2015-01-01

A DC-DC converter for generating a DC output voltage includes: a digitally controlled pulse width modulator (DPWM) for controlling a switching power stage to supply a varying voltage to an inductor; and a digital voltage feedback circuit for controlling the DPWM in accordance with a feedback voltage corresponding to the DC output voltage, the digital voltage feedback circuit including: a first voltage controlled oscillator for converting the feedback voltage into a first frequency signal and to supply the first frequency signal to a first frequency discriminator; a second voltage controlled oscillator for converting a reference voltage into a second frequency signal and to supply the second frequency signal to a second frequency discriminator; a digital comparator for comparing digital outputs of the first and second frequency discriminators and for outputting a digital feedback signal; and a controller for controlling the DPWM in accordance with the digital feedback signal.

Distributed measurement of acoustic vibration location with frequency multiplexed phase-OTDR

NASA Astrophysics Data System (ADS)

Iida, Daisuke; Toge, Kunihiro; Manabe, Tetsuya

2017-07-01

All-fiber distributed vibration sensing is attracting attention in relation to structural health monitoring because it is cost effective, offers high coverage of the monitored area and can detect various structural problems. And in particular the demand for high-speed vibration sensing operating at more than 10 kHz has increased because high frequency vibration indicates high energy and severe trouble in the monitored object. Optical fiber vibration sensing with phase-sensitive optical time domain reflectometry (phase-OTDR) has long been studied because it can be used for distributed vibration sensing in optical fiber. However, pulse reflectometry such as OTDR cannot measure high-frequency vibration whose cycle is shorter than the repetition time of the OTDR. That is, the maximum detectable frequency depends on fiber length. In this paper, we describe a vibration sensing technique with frequency-multiplexed OTDR that can detect the entire distribution of a high-frequency vibration thus allowing us to locate a high-speed vibration point. We can measure the position, frequency and dynamic change of a high-frequency vibration whose cycle is shorter than the repetition time. Both frequency and position are visualized simultaneously for a 5-km fiber with an 80-kHz frequency response and a 20-m spatial resolution.

A digital audio/video interleaving system. [for Shuttle Orbiter

NASA Technical Reports Server (NTRS)

Richards, R. W.

1978-01-01

A method of interleaving an audio signal with its associated video signal for simultaneous transmission or recording, and the subsequent separation of the two signals, is described. Comparisons are made between the new audio signal interleaving system and the Skylab Pam audio/video interleaving system, pointing out improvements gained by using the digital audio/video interleaving system. It was found that the digital technique is the simplest, most effective and most reliable method for interleaving audio and/or other types of data into the video signal for the Shuttle Orbiter application. Details of the design of a multiplexer capable of accommodating two basic data channels, each consisting of a single 31.5-kb/s digital bit stream are given. An adaptive slope delta modulation system is introduced to digitize audio signals, producing a high immunity of work intelligibility to channel errors, primarily due to the robust nature of the delta-modulation algorithm.

Optical Communication: Its History and Recent Progress

NASA Astrophysics Data System (ADS)

Agrawal, Govind P.

This chapter begins with a brief history of optical communication before describing the main components of a modern optical communication system. Specific attention is paid to the development of low-loss optical fibers as they played an essential role after 1975. The evolution of fiber-optic communication systems is described through its six generations over a 40-year time period ranging from 1975 to 2015. The adoption of wavelength-division multiplexing (WDM) during the 1990s to meet the demand fueled by the advent of the Internet is discussed together with the bursting of the telecom bubble in 2000. Recent advances brought by digital coherent technology and space-division multiplexing are also described briefly.

Holographic Compact Disk Read-Only Memories

NASA Technical Reports Server (NTRS)

Liu, Tsuen-Hsi

1996-01-01

Compact disk read-only memories (CD-ROMs) of proposed type store digital data in volume holograms instead of in surface differentially reflective elements. Holographic CD-ROM consist largely of parts similar to those used in conventional CD-ROMs. However, achieves 10 or more times data-storage capacity and throughput by use of wavelength-multiplexing/volume-hologram scheme.

47 CFR 76.602 - Incorporation by reference.

Code of Federal Regulations, 2014 CFR

2014-10-01

... MULTICHANNEL VIDEO AND CABLE TELEVISION SERVICE Technical Standards § 76.602 Incorporation by reference. (a...),” March 18, 2003, IBR approved for § 76.640. (2) ATSC A/85:2011 “ATSC Recommended Practice: Techniques for....640. (5) ANSI/SCTE 54 2003 (formerly DVS 241), “Digital Video Service Multiplex and Transport System...

Comparison of DFT-S-orthogonal frequency division multiplexing and single-carrier in a radio-over-fiber system

NASA Astrophysics Data System (ADS)

Wang, Kaihui; Li, Xinying; Yu, Jianjun

2017-09-01

DFT-S-orthogonal frequency division multiplexing (OFDM) and single-carrier (SC) modulation are two typical modulation formats in radio-over-fiber (RoF) systems. They may have respective advantages and disadvantages in different scenarios. Therefore, bit error ratio comparison results of these two modulation formats will be useful for designing and optimizing the practical RoF system. We experimentally compare these two modulation formats in a long wireless distance RoF system at W-band. It can be concluded that DFT-S-OFDM and SC modulation have similar performances in a RoF system with transmission distance over 80-km fiber and 224-m wireless link.

Development of high sensitivity eight-element multiplexed fiber laser acoustic pressure hydrophone array and interrogation system

NASA Astrophysics Data System (ADS)

Li, Ming; Sun, Zhihui; Zhang, Xiaolei; Li, Shujuan; Song, Zhiqiang; Wang, Meng; Guo, Jian; Ni, Jiasheng; Wang, Chang; Peng, Gangding; Xu, Xiangang

2017-09-01

Fiber laser hydrophones have got widespread concerns due to the unique advantages and broad application prospects. In this paper, the research results of the eight-element multiplexed fiber laser acoustic pressure array and the interrogation system are introduced, containing low-noise distributed feedback fiber laser (DFB-FL) fabrication, sensitivity enhancement packaging, and interferometric signal demodulation. The frequency response range of the system is 10Hz-10kHz, the laser frequency acoustic pressure sensitivity reaches 115 dB re Hz/Pa, and the equivalent noise acoustic pressure is less than 60μPa/Hz1/2. The dynamic range of the system is greater than 120 dB.

Engineering integrated digital circuits with allosteric ribozymes for scaling up molecular computation and diagnostics.

PubMed

Penchovsky, Robert

2012-10-19

Here we describe molecular implementations of integrated digital circuits, including a three-input AND logic gate, a two-input multiplexer, and 1-to-2 decoder using allosteric ribozymes. Furthermore, we demonstrate a multiplexer-decoder circuit. The ribozymes are designed to seek-and-destroy specific RNAs with a certain length by a fully computerized procedure. The algorithm can accurately predict one base substitution that alters the ribozyme's logic function. The ability to sense the length of RNA molecules enables single ribozymes to be used as platforms for multiple interactions. These ribozymes can work as integrated circuits with the functionality of up to five logic gates. The ribozyme design is universal since the allosteric and substrate domains can be altered to sense different RNAs. In addition, the ribozymes can specifically cleave RNA molecules with triplet-repeat expansions observed in genetic disorders such as oculopharyngeal muscular dystrophy. Therefore, the designer ribozymes can be employed for scaling up computing and diagnostic networks in the fields of molecular computing and diagnostics and RNA synthetic biology.

Programming time-multiplexed reconfigurable hardware using a scalable neuromorphic compiler.

PubMed

Minkovich, Kirill; Srinivasa, Narayan; Cruz-Albrecht, Jose M; Cho, Youngkwan; Nogin, Aleksey

2012-06-01

Scalability and connectivity are two key challenges in designing neuromorphic hardware that can match biological levels. In this paper, we describe a neuromorphic system architecture design that addresses an approach to meet these challenges using traditional complementary metal-oxide-semiconductor (CMOS) hardware. A key requirement in realizing such neural architectures in hardware is the ability to automatically configure the hardware to emulate any neural architecture or model. The focus for this paper is to describe the details of such a programmable front-end. This programmable front-end is composed of a neuromorphic compiler and a digital memory, and is designed based on the concept of synaptic time-multiplexing (STM). The neuromorphic compiler automatically translates any given neural architecture to hardware switch states and these states are stored in digital memory to enable desired neural architectures. STM enables our proposed architecture to address scalability and connectivity using traditional CMOS hardware. We describe the details of the proposed design and the programmable front-end, and provide examples to illustrate its capabilities. We also provide perspectives for future extensions and potential applications.

Digitally encoded DNA nanostructures for multiplexed, single-molecule protein sensing with nanopores

NASA Astrophysics Data System (ADS)

Bell, Nicholas A. W.; Keyser, Ulrich F.

2016-07-01

The simultaneous detection of a large number of different analytes is important in bionanotechnology research and in diagnostic applications. Nanopore sensing is an attractive method in this regard as the approach can be integrated into small, portable device architectures, and there is significant potential for detecting multiple sub-populations in a sample. Here, we show that highly multiplexed sensing of single molecules can be achieved with solid-state nanopores by using digitally encoded DNA nanostructures. Based on the principles of DNA origami, we designed a library of DNA nanostructures in which each member contains a unique barcode; each bit in the barcode is signalled by the presence or absence of multiple DNA dumbbell hairpins. We show that a 3-bit barcode can be assigned with 94% accuracy by electrophoretically driving the DNA structures through a solid-state nanopore. Select members of the library were then functionalized to detect a single, specific antibody through antigen presentation at designed positions on the DNA. This allows us to simultaneously detect four different antibodies of the same isotype at nanomolar concentration levels.

Digitally encoded DNA nanostructures for multiplexed, single-molecule protein sensing with nanopores.

PubMed

Bell, Nicholas A W; Keyser, Ulrich F

2016-07-01

The simultaneous detection of a large number of different analytes is important in bionanotechnology research and in diagnostic applications. Nanopore sensing is an attractive method in this regard as the approach can be integrated into small, portable device architectures, and there is significant potential for detecting multiple sub-populations in a sample. Here, we show that highly multiplexed sensing of single molecules can be achieved with solid-state nanopores by using digitally encoded DNA nanostructures. Based on the principles of DNA origami, we designed a library of DNA nanostructures in which each member contains a unique barcode; each bit in the barcode is signalled by the presence or absence of multiple DNA dumbbell hairpins. We show that a 3-bit barcode can be assigned with 94% accuracy by electrophoretically driving the DNA structures through a solid-state nanopore. Select members of the library were then functionalized to detect a single, specific antibody through antigen presentation at designed positions on the DNA. This allows us to simultaneously detect four different antibodies of the same isotype at nanomolar concentration levels.

Wavelength-multiplexing surface plasmon holographic microscopy.

PubMed

Zhang, Jiwei; Dai, Siqing; Zhong, Jinzhan; Xi, Teli; Ma, Chaojie; Li, Ying; Di, Jianglei; Zhao, Jianlin

2018-05-14

Surface plasmon holographic microscopy (SPHM), which combines surface plasmon microscopy with digital holographic microscopy, can be applied for amplitude- and phase-contrast surface plasmon resonance (SPR) imaging. In this paper, we propose an improved SPHM with the wavelength multiplexing technique based on two laser sources and a common-path hologram recording configuration. Through recording and reconstructing the SPR images at two wavelengths simultaneously employing the improved SPHM, tiny variation of dielectric refractive index in near field is quantitatively monitored with an extended measurement range while maintaining the high sensitivity. Moreover, imaging onion tissues is performed to demonstrate that the detection sensitivities of two wavelengths can compensate for each other in SPR imaging. The proposed wavelength-multiplexing SPHM presents simple structure, high temporal stability and inherent capability of phase curvature compensation, as well as shows great potentials for further applications in monitoring diverse dynamic processes related with refractive index variations and imaging biological tissues with low-contrast refractive index distributions in the near field.

Enhancements to a Superconducting Quantum Interference Device (SQUID) Multiplexer Readout and Control System

NASA Technical Reports Server (NTRS)

Forgione, J.; Benford, D. J.; Buchanan, E. D.; Moseley, S. H.; Rebar, J.; Shafer, R. A.

2004-01-01

Far-infrared detector arrays such as the 16x32 superconducting bolometer array for the SAFIRE instrument (flying on the SOFIA airborne observatory) require systems of readout and control electronics to provide translation between a user-driven, digital PC and the cold, analog world of the cryogenic detector. In 2001, the National Institute of Standards and Technology (NIST) developed their Mark III electronics for purposes of control and readout of their 1x32 SQUID Multiplexer chips. We at NASA s Goddard Space Flight Center acquired a Mark 111 system and subsequently designed upgrades to suit our and our collaborators purposes. We developed an arbitrary, programmable multiplexing system that allows the user to cycle through rows in a SQUID array in an infinite number of combinations. We provided hooks in the Mark III system to allow readout of signals from outside the Mark 111 system, such as telescope status information. Finally, we augmented the heart of the system with a new feedback algorithm implementation, flexible diagnostic tools, and informative telemetry.

80-Channel Multiplexer-Demultiplexer Module for DWDM Communications using Hybrid AWG -- Interleaver Technology

NASA Astrophysics Data System (ADS)

Rablau, Corneliu; Bredthauer, Lance

2007-10-01

Aside from the more traditional data, voice and e-mail communications, new bandwidth intensive applications in the larger consumer markets, such as music, digital pictures and movies, have led to an explosive increase in the demand for transmission capacity for optical communications networks. This has resulted in a widespread deployment of Dense Wavelength Division Multiplexing (DWDM) as a means of increasing the communications capacity by multiplexing and transmitting signals of different wavelengths (establishing multiple communication channels) through a single strand of fiber. We report on the design, assembly and characterization of a 50-GHz, 80-channel Mux-Demux module for DWDM systems. The module has been assembled from two commercially available 100 GHz, 40-channel Array Waveguide Grating (AWG) modules and a 50-GHz to 100-GHz interleaver. Relevant performance parameters such as insertion loss, channel uniformity, next-channel isolation (crosstalk) and integrated cross-talk are presented and discussed in contrast with the performance of other competing technologies such as Thin-Film-Filter-based Mux-Demux devices.

A 90GHz Bolometer Camera Detector System for the Green Bank Telescope

NASA Technical Reports Server (NTRS)

Benford, Dominic J.; Allen, Christine A.; Buchanan, Ernest D.; Chen, Tina C.; Chervenak, James A.; Devlin, Mark J.; Dicker, Simon R.; Forgione, Joshua B.

2004-01-01

We describe a close-packed, two-dimensional imaging detector system for operation at 90GHz (3.3mm) for the 100 m Green Bank Telescope (GBT) This system will provide high sensitivity (<1mjy in 1s rapid imaging (15'x15' to 250 microJy in 1 hr) at the world's largest steerable aperture. The heart of this camera is an 8x8 close packed, Nyquist-sampled array of superconducting transition edge sensor bolometers. We have designed and are producing a functional superconducting bolometer array system using a monolithic planar architecture and high-speed multiplexed readout electronics. With an NEP of approx. 2.10(exp 17) W/square root Hz, the TES bolometers will provide fast linear sensitive response for high performance imaging. The detectors are read out by and 8x8 time domain SQUID multiplexer. A digital/analog electronics system has been designed to enable read out by SQUID multiplexers. First light for this instrument on the GBT is expected within a year.

A 90GHz Bolometer Camera Detector System for the Green

NASA Technical Reports Server (NTRS)

Benford, Dominic J.; Allen, Christine A.; Buchanan, Ernest; Chen, Tina C.; Chervenak, James A.; Devlin, Mark J.; Dicker, Simon R.; Forgione, Joshua B.

2004-01-01

We describe a close-packed, two-dimensional imaging detector system for operation at 90GHz (3.3 mm) for the 100m Green Bank Telescope (GBT). This system will provide high sensitivity (less than 1mJy in 1s) rapid imaging (15'x15' to 150 micron Jy in 1 hr) at the world's largest steerable aperture. The heart of this camera is an 8x8 close-packed, Nyquist-sampled array of superconducting transition edge sensor (TES) bolometers. We have designed and are producing a functional superconducting bolometer array system using a monolithic planar architecture and high-speed multiplexed readout electronics. With an NEP of approximately 2 x 10(exp -17) W/square root of Hz, the TES bolometers will provide fast, linear, sensitive response for high performance imaging. The detectors are read out by an 8x8 time domain SQUID multiplexer. A digital/analog electronics system has been designed to enable read out by SQUID multiplexers. First light for this instrument on the GBT is expected within a year.

Quantitative multiplex immunohistochemistry reveals myeloid-inflamed tumor-immune complexity associated with poor prognosis

PubMed Central

Tsujikawa, Takahiro; Kumar, Sushil; Borkar, Rohan N.; Azimi, Vahid; Thibault, Guillaume; Chang, Young Hwan; Balter, Ariel; Kawashima, Rie; Choe, Gina; Sauer, David; El Rassi, Edward; Clayburgh, Daniel R.; Kulesz-Martin, Molly F.; Lutz, Eric R.; Zheng, Lei; Jaffee, Elizabeth M.; Leyshock, Patrick; Margolin, Adam A.; Mori, Motomi; Gray, Joe W.; Flint, Paul W.; Coussens, Lisa M.

2017-01-01

SUMMARY Here we describe a multiplexed immunohistochemical platform, with computational image processing workflows including image cytometry, enabling simultaneous evaluation of 12 biomarkers in one formalin-fixed paraffin-embedded tissue section. To validate this platform, we used tissue microarrays containing 38 archival head and neck squamous cell carcinomas, and revealed differential immune profiles based on lymphoid and myeloid cell densities, correlating with human papilloma virus status and prognosis. Based on these results, we investigated 24 pancreatic ductal adenocarcinomas from patients who received neoadjuvant GVAX vaccination, and revealed that response to therapy correlated with degree of mono-myelocytic cell density, and percentages of CD8+ T cells expressing T cell exhaustion markers. These data highlight the utility of in situ immune monitoring for patient stratification, and provide digital image processing pipelines (https://github.com/multiplexIHC/cppipe) to the community for examining immune complexity in precious tissue sections, where phenotype and tissue architecture are preserved to thus improve biomarker discovery and assessment. PMID:28380359

Enhancements to a superconducting quantum interference device (SQUID) multiplexer readout and control system

NASA Astrophysics Data System (ADS)

Forgione, Joshua B.; Benford, Dominic J.; Buchanan, Ernest D.; Moseley, S. H., Jr.; Rebar, Joyce; Shafer, Richard A.

2004-10-01

Far-infrared detector arrays such as the 16x32 superconducting bolometer array for the SAFIRE instrument (flying on the SOFIA airborne observatory) require systems of readout and control electronics to provide translation between a user-driven, digital PC and the cold, analog world of the cryogenic detector. In 2001, the National Institute of Standards and Technology (NIST) developed their Mark III electronics for purposes of control and readout of their 1x32 SQUID Multiplexer chips. We at NASA's Goddard Space Flight Center acquired a Mark III system and subsequently designed upgrades to suit our and our collaborators' purposes. We developed an arbitrary, programmable multiplexing system that allows the user to cycle through rows in a SQUID array in an infinite number of combinations. We provided 'hooks' in the Mark III system to allow readout of signals from outside the Mark III system, such as telescope status information. Finally, we augmented the heart of the system with a new feedback algorithm implementation, flexible diagnostic tools, and informative telemetry.

High-speed acoustic communication by multiplexing orbital angular momentum

PubMed Central

Shi, Chengzhi; Dubois, Marc; Wang, Yuan

2017-01-01

Long-range acoustic communication is crucial to underwater applications such as collection of scientific data from benthic stations, ocean geology, and remote control of off-shore industrial activities. However, the transmission rate of acoustic communication is always limited by the narrow-frequency bandwidth of the acoustic waves because of the large attenuation for high-frequency sound in water. Here, we demonstrate a high-throughput communication approach using the orbital angular momentum (OAM) of acoustic vortex beams with one order enhancement of the data transmission rate at a single frequency. The topological charges of OAM provide intrinsically orthogonal channels, offering a unique ability to multiplex data transmission within a single acoustic beam generated by a transducer array, drastically increasing the information channels and capacity of acoustic communication. A high spectral efficiency of 8.0 ± 0.4 (bit/s)/Hz in acoustic communication has been achieved using topological charges between −4 and +4 without applying other communication modulation techniques. Such OAM is a completely independent degree of freedom which can be readily integrated with other state-of-the-art communication modulation techniques like quadrature amplitude modulation (QAM) and phase-shift keying (PSK). Information multiplexing through OAM opens a dimension for acoustic communication, providing a data transmission rate that is critical for underwater applications. PMID:28652341

Physically secured orthogonal frequency division multiplexing-passive optical network employing noise-based encryption and signal recovery process

NASA Astrophysics Data System (ADS)

Jin, Wei; Zhang, Chongfu; Yuan, Weicheng

2016-02-01

We propose a physically enhanced secure scheme for direct detection-orthogonal frequency division multiplexing-passive optical network (DD-OFDM-PON) and long reach coherent detection-orthogonal frequency division multiplexing-passive optical network (LRCO-OFDM-PON), by employing noise-based encryption and channel/phase estimation. The noise data generated by chaos mapping are used to substitute training sequences in preamble to realize channel estimation and frame synchronization, and also to be embedded on variable number of key-selected randomly spaced pilot subcarriers to implement phase estimation. Consequently, the information used for signal recovery is totally hidden as unpredictable noise information in OFDM frames to mask useful information and to prevent illegal users from correctly realizing OFDM demodulation, and thereby enhancing resistance to attackers. The levels of illegal-decryption complexity and implementation complexity are theoretically discussed. Through extensive simulations, the performances of the proposed channel/phase estimation and the security introduced by encrypted pilot carriers have been investigated in both DD-OFDM and LRCO-OFDM systems. In addition, in the proposed secure DD-OFDM/LRCO-OFDM PON models, both legal and illegal receiving scenarios have been considered. These results show that, by utilizing the proposed scheme, the resistance to attackers can be significantly enhanced in DD-OFDM-PON and LRCO-OFDM-PON systems without performance degradations.

Rapid diagnosis of sepsis with TaqMan-Based multiplex real-time PCR.

PubMed

Liu, Chang-Feng; Shi, Xin-Ping; Chen, Yun; Jin, Ye; Zhang, Bing

2018-02-01

The survival rate of septic patients mainly depends on a rapid and reliable diagnosis. A rapid, broad range, specific and sensitive quantitative diagnostic test is the urgent need. Thus, we developed a TaqMan-Based Multiplex real-time PCR assays to identify bloodstream pathogens within a few hours. Primers and TaqMan probes were designed to be complementary to conserved regions in the 16S rDNA gene of different kinds of bacteria. To evaluate accurately, sensitively, and specifically, the known bacteria samples (Standard strains, whole blood samples) are determined by TaqMan-Based Multiplex real-time PCR. In addition, 30 blood samples taken from patients with clinical symptoms of sepsis were tested by TaqMan-Based Multiplex real-time PCR and blood culture. The mean frequency of positive for Multiplex real-time PCR was 96% at a concentration of 100 CFU/mL, and it was 100% at a concentration greater than 1000 CFU/mL. All the known blood samples and Standard strains were detected positively by TaqMan-Based Multiplex PCR, no PCR products were detected when DNAs from other bacterium were used in the multiplex assay. Among the 30 patients with clinical symptoms of sepsis, 18 patients were confirmed positive by Multiplex real-time PCR and seven patients were confirmed positive by blood culture. TaqMan-Based Multiplex real-time PCR assay with highly sensitivity, specificity and broad detection range, is a rapid and accurate method in the detection of bacterial pathogens of sepsis and should have a promising usage in the diagnosis of sepsis. © 2017 Wiley Periodicals, Inc.

A filter circuit board for the Earthworm Seismic Data Acquisition System

USGS Publications Warehouse

Jensen, Edward Gray

2000-01-01

The Earthworm system is a seismic network data acquisition and processing system used by the Northern California Seismic Network as well as many other seismic networks. The input to the system is comprised of many realtime electronic waveforms fed to a multi-channel digitizer on a PC platform. The digitizer consists of one or more National Instruments Corp. AMUX–64T multiplexer boards attached to an A/D converter board located in the computer. Originally, passive filters were installed on the multiplexers to eliminate electronic noise picked up in cabling. It was later discovered that a small amount of crosstalk occurred between successive channels in the digitizing sequence. Though small, this crosstalk will cause what appear to be small earthquake arrivals at the wrong time on some channels. This can result in erroneous calculation of earthquake arrival times, particularly by automated algorithms. To deal with this problem, an Earthworm filter board was developed to provide the needed filtering while eliminating crosstalk. This report describes the tests performed to find a suitable solution, and the design of the circuit board. Also included are all the details needed to build and install this board in an Earthworm system or any other system using the AMUX–64T board. Available below is the report in PDF format as well as an archive file containing the circuit board manufacturing information.

Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.

PubMed

Deng, Lili; Qi, Zongtai; Zou, Binjie; Wu, Haiping; Huang, Huan; Kajiyama, Tomoharu; Kambara, Hideki; Zhou, Guohua

2012-07-03

Somatic mutations in stool DNA are quite specific to colorectal cancer (CRC), but a method being able to detect the extraordinarily low amounts of mutants is challengeable in sensitivity. We proposed a hydrogel bead-array to digitally count CRC-specific mutants in stool at a low cost. At first, multiplex amplification of targets containing multiple mutation loci of interest is carried out by a target enriched multiplex PCR (Tem-PCR), yielding the templates qualified for emulsion PCR (emPCR). Then, after immobilizing the beads from emPCR on a glass surface, the incorporation of Cy3-dUTP into the mutant-specific probes, which are specifically hybridized with the amplified beads from emPCR, is used to color the beads coated with mutants. As all amplified beads are hybridized with the Cy5-labeled universal probe, a mutation rate is readily obtained by digitally counting the beads with different colors (yellow and red). A high specificity of the method is achieved by removing the mismatched probes in a bead-array with electrophoresis. The approach has been used to simultaneously detect 8 mutation loci within the APC, TP53, and KRAS genes in stools from eight CRC patients, and 50% of CRC patients were positively diagnosed; therefore, our method can be a potential tool for the noninvasive diagnosis of CRC.

Helicity multiplexed broadband metasurface holograms.

PubMed

Wen, Dandan; Yue, Fuyong; Li, Guixin; Zheng, Guoxing; Chan, Kinlong; Chen, Shumei; Chen, Ming; Li, King Fai; Wong, Polis Wing Han; Cheah, Kok Wai; Pun, Edwin Yue Bun; Zhang, Shuang; Chen, Xianzhong

2015-09-10

Metasurfaces are engineered interfaces that contain a thin layer of plasmonic or dielectric nanostructures capable of manipulating light in a desirable manner. Advances in metasurfaces have led to various practical applications ranging from lensing to holography. Metasurface holograms that can be switched by the polarization state of incident light have been demonstrated for achieving polarization multiplexed functionalities. However, practical application of these devices has been limited by their capability for achieving high efficiency and high image quality. Here we experimentally demonstrate a helicity multiplexed metasurface hologram with high efficiency and good image fidelity over a broad range of frequencies. The metasurface hologram features the combination of two sets of hologram patterns operating with opposite incident helicities. Two symmetrically distributed off-axis images are interchangeable by controlling the helicity of the input light. The demonstrated helicity multiplexed metasurface hologram with its high performance opens avenues for future applications with functionality switchable optical devices.

Helicity multiplexed broadband metasurface holograms

PubMed Central

Wen, Dandan; Yue, Fuyong; Li, Guixin; Zheng, Guoxing; Chan, Kinlong; Chen, Shumei; Chen, Ming; Li, King Fai; Wong, Polis Wing Han; Cheah, Kok Wai; Yue Bun Pun, Edwin; Zhang, Shuang; Chen, Xianzhong

2015-01-01

Metasurfaces are engineered interfaces that contain a thin layer of plasmonic or dielectric nanostructures capable of manipulating light in a desirable manner. Advances in metasurfaces have led to various practical applications ranging from lensing to holography. Metasurface holograms that can be switched by the polarization state of incident light have been demonstrated for achieving polarization multiplexed functionalities. However, practical application of these devices has been limited by their capability for achieving high efficiency and high image quality. Here we experimentally demonstrate a helicity multiplexed metasurface hologram with high efficiency and good image fidelity over a broad range of frequencies. The metasurface hologram features the combination of two sets of hologram patterns operating with opposite incident helicities. Two symmetrically distributed off-axis images are interchangeable by controlling the helicity of the input light. The demonstrated helicity multiplexed metasurface hologram with its high performance opens avenues for future applications with functionality switchable optical devices. PMID:26354497

Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay.

PubMed

Zar, Mian Sahib; Shahid, Ahmad Ali; Shahzad, Muhammad Saqib; Shin, Kyoung-Jin; Lee, Hwan Young; Lee, Sang-Seob; Israr, Muhammad; Wiegand, Peter; Kulstein, Galina

2018-04-10

This study introduces a newly developed in-house SNaPshot single-base extension (SBE) multiplex assay for forensic single nucleotide polymorphism (SNP) genotyping of fresh and degraded samples. The assay was validated with fresh blood samples from four different populations. In addition, altogether 24 samples from skeletal remains were analyzed with the multiplex. Full SNP profiles could be obtained from 14 specimens, while ten remains showed partial SNP profiles. Minor allele frequencies (MAF) of bone samples and different populations were compared and used for association of skeletal remains with a certain population. The results reveal that the SNPs of the bone samples are genetically close to the Pathan population. The findings show that the new multiplex system can be utilized for SNP genotyping of degraded and forensic relevant skeletal material, enabling to provide additional investigative leads in criminal cases. © 2018 American Academy of Forensic Sciences.

Polarization-multiplexed 2×2 phosphor-LED wireless light communication without using analog equalization and optical blue filter

NASA Astrophysics Data System (ADS)

Yeh, C. H.; Chen, H. Y.; Liu, Y. L.; Chow, C. W.

2015-01-01

We propose and experimentally demonstrate a 380 (2×190) Mbps phosphor-light-emitting-diode (LED) based visible light communication (VLC) system by using 2×2 polarization-multiplexing design for in-building access applications. To the best of our knowledge, this is the first time of employing polarization-multiplexing to achieve a high VLC transmission capacity by using phosphor-based white-LED without optical blue filter. Besides, utilizing the optimum resistor-inductor-capacity (RLC) bias-tee design, it can not only perform the function of combining the direct-current (DC) and the electrical data signal, but also act as a simple LED-Tx circuit. No optical blue filter and complicated post-equalization are required at the Rx. Here, the orthogonal-frequency-division-multiplexing (OFDM) quadrature-amplitude-modulation (QAM) with bit-loading is employed to enhance the transmission data rate.

DOE Office of Scientific and Technical Information (OSTI.GOV)

De Geronimo, G.; Li, S.; D'Andragora, A.

We present a front-end application-specific integrated circuit (ASIC) for a wire based time-projection-chamber (TPC) operating in liquid Argon (LAr). The LAr TPC will be used for long baseline neutrino oscillation experiments. The ASIC must provide a low-noise readout of the signals induced on the TPC wires, digitization of those signals at 2 MSamples/s, compression, buffering and multiplexing. A resolution of better than 1000 rms electrons at 200 pF input capacitance for an input range of 300 fC is required, along with low power and operation in LAr (at 87 K). We include the characterization of a commercial technology for operationmore » in the cryogenic environment and the first experimental results on the analog front end. The results demonstrate that complementary metal-oxide semiconductor transistors have lower noise and much improved dc characteristics at LAr temperature. Finally, we introduce the concept of '1/f equivalent' to model the low-frequency component of the noise spectral density, for use in the input metal-oxide semiconductor field-effect transistor optimization.« less

The E and B EXperiment: EBEX

NASA Astrophysics Data System (ADS)

Helson, Kyle

2014-03-01

We report on the status of the E and B Experiment (EBEX) a balloon-borne polarimeter designed to measure the polarization of the cosmic microwave background radiation. The instrument employs a 1.5 meter Gregorian Mizuguchi-Dragone telescope providing 8 arc-minute resolution at three bands centered on 150, 250, and 410 GHz. A continuously rotating achromatic half wave plate, mounted on a superconducting magnetic bearing, and a polarizing grid give EBEX polarimetric capabilities. Radiation is detected with a kilo-pixel array of transition edge sensor (TES) bolometers that are cooled to 0.25 K. The detectors are readout using SQUID current amplifiers and a digital frequency-domain multiplexing system in which 16 detectors are readout simultaneously with two wires. EBEX is the first instrument to implement TESs and such readout system on board a balloon-borne platform. EBEX was launched from the Antarctic in December 2012 on an 11-day long-duration balloon flight. This presentation will provide an overview of the instrument and discuss the flight and status of the data analysis.

A Millimeter-Wave Digital Link for Wireless MRI

PubMed Central

Aggarwal, Kamal; Joshi, Kiran R.; Rajavi, Yashar; Taghivand, Mazhareddin; Pauly, John M.; Poon, Ada S. Y.; Scott, Greig

2017-01-01

A millimeter (mm) wave radio is presented in this work to support wireless MRI data transmission. High path loss and availability of wide bandwidth make mm-waves an ideal candidate for short range, high data rata communication required for wireless MRI. The proposed system uses a custom designed integrated chip (IC) mm-wave radio with 60 GHz as radio frequency carrier. In this work, we assess performance in a 1.5 T MRI field, with the addition of optical links between the console room and magnet. The system uses ON-OFF keying (OOK) modulation for data transmission and supports data rates from 200 Mb/s to 2.5 Gb/s for distances up-to 65 cm. The presence of highly directional, linearly polarized, on-chip dipole antennas on the mm-wave radio along with the time division multiplexing (TDM) circuitry allows multiple wireless links to be created simultaneously with minimal inter-channel interference. This leads to a highly scalable solution for wireless MRI. PMID:27810803

A two layer chaotic encryption scheme of secure image transmission for DCT precoded OFDM-VLC transmission

NASA Astrophysics Data System (ADS)

Wang, Zhongpeng; Chen, Fangni; Qiu, Weiwei; Chen, Shoufa; Ren, Dongxiao

2018-03-01

In this paper, a two-layer image encryption scheme for a discrete cosine transform (DCT) precoded orthogonal frequency division multiplexing (OFDM) visible light communication (VLC) system is proposed. Firstly, in the proposed scheme the transmitted image is first encrypted by a chaos scrambling sequence,which is generated from the hybrid 4-D hyper- and Arnold map in the upper-layer. After that, the encrypted image is converted into digital QAM modulation signal, which is re-encrypted by chaos scrambling sequence based on Arnold map in physical layer to further enhance the security of the transmitted image. Moreover, DCT precoding is employed to improve BER performance of the proposed system and reduce the PAPR of OFDM signal. The BER and PAPR performances of the proposed system are evaluated by simulation experiments. The experiment results show that the proposed two-layer chaos scrambling schemes achieve image secure transmission for image-based OFDM VLC. Furthermore, DCT precoding can reduce the PAPR and improve the BER performance of OFDM-based VLC.

A Millimeter-Wave Digital Link for Wireless MRI.

PubMed

Aggarwal, Kamal; Joshi, Kiran R; Rajavi, Yashar; Taghivand, Mazhareddin; Pauly, John M; Poon, Ada S Y; Scott, Greig

2017-02-01

A millimeter (mm) wave radio is presented in this work to support wireless MRI data transmission. High path loss and availability of wide bandwidth make mm-waves an ideal candidate for short range, high data rata communication required for wireless MRI. The proposed system uses a custom designed integrated chip (IC) mm-wave radio with 60 GHz as radio frequency carrier. In this work, we assess performance in a 1.5 T MRI field, with the addition of optical links between the console room and magnet. The system uses ON-OFF keying (OOK) modulation for data transmission and supports data rates from 200 Mb/s to 2.5 Gb/s for distances up-to 65 cm. The presence of highly directional, linearly polarized, on-chip dipole antennas on the mm-wave radio along with the time division multiplexing (TDM) circuitry allows multiple wireless links to be created simultaneously with minimal inter-channel interference. This leads to a highly scalable solution for wireless MRI.

Development and experimental validation of downlink multiuser MIMO-OFDM in gigabit wireless LAN systems

NASA Astrophysics Data System (ADS)

Ishihara, Koichi; Asai, Yusuke; Kudo, Riichi; Ichikawa, Takeo; Takatori, Yasushi; Mizoguchi, Masato

2013-12-01

Multiuser multiple-input multiple-output (MU-MIMO) has been proposed as a means to improve spectrum efficiency for various future wireless communication systems. This paper reports indoor experimental results obtained for a newly developed and implemented downlink (DL) MU-MIMO orthogonal frequency division multiplexing (OFDM) transceiver for gigabit wireless local area network systems in the microwave band. In the transceiver, the channel state information (CSI) is estimated at each user and fed back to an access point (AP) on a real-time basis. At the AP, the estimated CSI is used to calculate the transmit beamforming weight for DL MU-MIMO transmission. This paper also proposes a recursive inverse matrix computation scheme for computing the transmit weight in real time. Experiments with the developed transceiver demonstrate its feasibility in a number of indoor scenarios. The experimental results clarify that DL MU-MIMO-OFDM transmission can achieve a 972-Mbit/s transmission data rate with simple digital signal processing of single-antenna users in an indoor environment.

Frequency division multiplexed radio-over-fiber transmission using an optically injected laser diode

NASA Astrophysics Data System (ADS)

Chan, Sze-Chun

2008-04-01

Nonlinear dynamics of semiconductor lasers have recently attracted much attention in the area of microwave photonics. By invoking the nonlinear dynamics of an optically injected laser diode, high-speed microwave oscillation can be generated using the period-one oscillation state. The oscillation is harnessed for application as a photonic microwave source in radio-over-fiber (RoF) systems. It is advantageous over conventional direct current modulation because it alleviates the modulation bandwidth limitation and naturally generates single sideband signals. The method is thus applicable to wireless communication systems even when the subcarrier frequency increases to 60 GHz. Because RoF is usually incorporated with standard wireless schemes that involve frequency division multiplexing (FDM), we investigate the performance of the optical injection system under simultaneous current injection of multiple data streams. Frequency mixings and competition for locking among subcarriers result in intermodulation distortion (IMD). The relative weightings of different channels should be optimized to ensure acceptable signal qualities. The results illustrate the feasibility of applying the optical injection system for FDM RoF transmission at high subcarrier frequencies.

Wavelength-stepped, actively mode-locked fiber laser based on wavelength-division-multiplexed optical delay lines

NASA Astrophysics Data System (ADS)

Lee, Eunjoo; Kim, Byoung Yoon

2017-12-01

We propose a new scheme for an actively mode-locked wavelength-swept fiber laser that produces a train of discretely wavelength-stepped pulses from a short fiber cavity. Pulses with different wavelengths are split and combined by standard wavelength division multiplexers with fiber delay lines. As a proof of concept, we demonstrate a laser using an erbium doped fiber amplifier and commercially available wavelength-division multiplexers with wavelength spacing of 0.8 nm. The results show simultaneous mode-locking at three different wavelengths. Laser output parameters in time domain, optical and radio frequency spectral domain, and the noise characteristics are presented. Suggestions for the improved design are discussed.

Recent advances in high-capacity free-space optical and radio-frequency communications using orbital angular momentum multiplexing.

PubMed

Willner, Alan E; Ren, Yongxiong; Xie, Guodong; Yan, Yan; Li, Long; Zhao, Zhe; Wang, Jian; Tur, Moshe; Molisch, Andreas F; Ashrafi, Solyman

2017-02-28

There is a continuing growth in the demand for data bandwidth, and the multiplexing of multiple independent data streams has the potential to provide the needed data capacity. One technique uses the spatial domain of an electromagnetic (EM) wave, and space division multiplexing (SDM) has become increasingly important for increased transmission capacity and spectral efficiency of a communication system. A subset of SDM is mode division multiplexing (MDM), in which multiple orthogonal beams each on a different mode can be multiplexed. A potential modal basis set to achieve MDM is to use orbital angular momentum (OAM) of EM waves. In such a system, multiple OAM beams each carrying an independent data stream are multiplexed at the transmitter, propagate through a common medium and are demultiplexed at the receiver. As a result, the total capacity and spectral efficiency of the communication system can be multiplied by a factor equal to the number of transmitted OAM modes. Over the past few years, progress has been made in understanding the advantages and limitations of using multiplexed OAM beams for communication systems. In this review paper, we highlight recent advances in the use of OAM multiplexing for high-capacity free-space optical and millimetre-wave communications. We discuss different technical challenges (e.g. atmospheric turbulence and crosstalk) as well as potential techniques to mitigate such degrading effects.This article is part of the themed issue 'Optical orbital angular momentum'. © 2017 The Author(s).

Recent advances in high-capacity free-space optical and radio-frequency communications using orbital angular momentum multiplexing

PubMed Central

Ren, Yongxiong; Xie, Guodong; Yan, Yan; Li, Long; Zhao, Zhe; Wang, Jian; Tur, Moshe; Molisch, Andreas F.; Ashrafi, Solyman

2017-01-01

There is a continuing growth in the demand for data bandwidth, and the multiplexing of multiple independent data streams has the potential to provide the needed data capacity. One technique uses the spatial domain of an electromagnetic (EM) wave, and space division multiplexing (SDM) has become increasingly important for increased transmission capacity and spectral efficiency of a communication system. A subset of SDM is mode division multiplexing (MDM), in which multiple orthogonal beams each on a different mode can be multiplexed. A potential modal basis set to achieve MDM is to use orbital angular momentum (OAM) of EM waves. In such a system, multiple OAM beams each carrying an independent data stream are multiplexed at the transmitter, propagate through a common medium and are demultiplexed at the receiver. As a result, the total capacity and spectral efficiency of the communication system can be multiplied by a factor equal to the number of transmitted OAM modes. Over the past few years, progress has been made in understanding the advantages and limitations of using multiplexed OAM beams for communication systems. In this review paper, we highlight recent advances in the use of OAM multiplexing for high-capacity free-space optical and millimetre-wave communications. We discuss different technical challenges (e.g. atmospheric turbulence and crosstalk) as well as potential techniques to mitigate such degrading effects. This article is part of the themed issue ‘Optical orbital angular momentum’. PMID:28069770

Coinfection of human herpesviruses 6A (HHV-6A) and HHV-6B as demonstrated by novel digital droplet PCR assay.

PubMed

Leibovitch, Emily C; Brunetto, Giovanna S; Caruso, Breanna; Fenton, Kaylan; Ohayon, Joan; Reich, Daniel S; Jacobson, Steven

2014-01-01

The human herpesviruses HHV-6A and HHV-6B have been associated with various neurologic disorders partly due to the detection of elevated viral DNA levels in patients compared to controls. However the reported frequency of these viruses varies widely, likely reflecting differences in PCR methodologies used for detection. Digital droplet PCR (ddPCR) is a third generation PCR technology that enables the absolute quantification of target DNA molecules. Mounting evidence of the biological differences between HHV-6A and HHV-6B has led to their recent reclassification as separate species. As it is now especially relevant to investigate each virus, our objectives were to first design a multiplex HHV-6A and HHV-6B ddPCR assay and then to investigate the incidence of HHV-6A and HHV-6B coinfection in samples from healthy donors and patients with MS, a disease in which HHV-6 is thought to play a role. In our assessment of healthy donors, we observed a heretofore-underappreciated high frequency of coinfection in PBMC and serum, and found that our assay precisely detects both HHV-6A and HHV-6B chromosomally integrated virus, which has important implications in clinical settings. Interestingly, upon comparing the saliva from MS patients and healthy donors, we detected a significantly elevated frequency of coinfection in MS saliva; increased detection of HHV-6A in MS patients is consistent with other studies suggesting that this viral species (thought to be more neurotropic than HHV-6B) is more prevalent among MS patients compared to healthy donors. As the biology and disease associations between these two viral species differ, identifying and quantifying both species of HHV-6 may provide clinically relevant information, as well as enhance our understanding of the roles of each in health and disease.

FPGA-based multi-channel fluorescence lifetime analysis of Fourier multiplexed frequency-sweeping lifetime imaging

PubMed Central

Zhao, Ming; Li, Yu; Peng, Leilei

2014-01-01

We report a fast non-iterative lifetime data analysis method for the Fourier multiplexed frequency-sweeping confocal FLIM (Fm-FLIM) system [ Opt. Express22, 10221 ( 2014)24921725]. The new method, named R-method, allows fast multi-channel lifetime image analysis in the system’s FPGA data processing board. Experimental tests proved that the performance of the R-method is equivalent to that of single-exponential iterative fitting, and its sensitivity is well suited for time-lapse FLIM-FRET imaging of live cells, for example cyclic adenosine monophosphate (cAMP) level imaging with GFP-Epac-mCherry sensors. With the R-method and its FPGA implementation, multi-channel lifetime images can now be generated in real time on the multi-channel frequency-sweeping FLIM system, and live readout of FRET sensors can be performed during time-lapse imaging. PMID:25321778

Multi-pixel high-resolution three-dimensional imaging radar

NASA Technical Reports Server (NTRS)

Cooper, Ken B. (Inventor); Dengler, Robert J. (Inventor); Siegel, Peter H. (Inventor); Chattopadhyay, Goutam (Inventor); Ward, John S. (Inventor); Juan, Nuria Llombart (Inventor); Bryllert, Tomas E. (Inventor); Mehdi, Imran (Inventor); Tarsala, Jan A. (Inventor)

2012-01-01

A three-dimensional imaging radar operating at high frequency e.g., 670 GHz radar using low phase-noise synthesizers and a fast chirper to generate a frequency-modulated continuous-wave (FMCW) waveform, is disclosed that operates with a multiplexed beam to obtain range information simultaneously on multiple pixels of a target. A source transmit beam may be divided by a hybrid coupler into multiple transmit beams multiplexed together and directed to be reflected off a target and return as a single receive beam which is demultiplexed and processed to reveal range information of separate pixels of the target associated with each transmit beam simultaneously. The multiple transmit beams may be developed with appropriate optics to be temporally and spatially differentiated before being directed to the target. Temporal differentiation corresponds to a different intermediate frequencies separating the range information of the multiple pixels. Collinear transmit beams having differentiated polarizations may also be implemented.

Development of a four-frequency selective surface prototype spacecraft antenna

NASA Astrophysics Data System (ADS)

Hickey, Gregory S.; Wu, Te-Kao

NASA-JPL's four-frequency telecommunication system design entails the creation and integration of a frequency-selective surface (FSS) subreflector into the high-gain antenna subsystem. The FSS design, which incorporates a periodic array of conducting elements on a kevlar/polymer composite structure, will be able to multiplex S, X, Ku, and Ka frequency-band wavelengths. Accounts are presented of the FSS's development, mechanical testing, and electrical testing.

High Resolution Imaging with MUSTANG-2 on the GBT

NASA Astrophysics Data System (ADS)

Stanchfield, Sara; Ade, Peter; Aguirre, James; Brevik, Justus A.; Cho, Hsiao-Mei; Datta, Rahul; Devlin, Mark; Dicker, Simon R.; Dober, Bradley; Duff, Shannon M.; Egan, Dennis; Ford, Pam; Hilton, Gene; Hubmayr, Johannes; Irwin, Kent; Knowles, Kenda; Marganian, Paul; Mason, Brian Scott; Mates, John A. B.; McMahon, Jeff; Mello, Melinda; Mroczkowski, Tony; Romero, Charles; Sievers, Jonathon; Tucker, Carole; Vale, Leila R.; Vissers, Michael; White, Steven; Whitehead, Mark; Ullom, Joel; Young, Alexander

2018-01-01

We present early science results from MUSTANG-2, a 90 GHz feedhorn-coupled, microwave SQUID-multiplexed TES bolometer array operating on the Robert C. Byrd Green Bank Telescope (GBT). The feedhorn and waveguide-probe-coupled detector technology is a mature technology, which has been used on instruments such as the South Pole Telescope, the Atacama Cosmology Telescope, and the Atacama B-mode Search telescope. The microwave SQUID multiplexer-based readout system developed for MUSTANG-2 currently reads out 66 detectors with a single coaxial cable and will eventually allow thousands of detectors to be multiplexed. This microwave SQUID multiplexer combines the proven abilities of millimeter wave TES detectors with the multiplexing capabilities of KIDs with no degradation in noise performance of the detectors. Each multiplexing device is read out using warm electronics consisting of a commercially available ROACH board, a DAC/ADC card, and an Intermediate Frequency mixer circuit. The hardware was originally developed by the Collaboration for Astronomy Signal Processing and Electronic Research (CASPER) group, whose primary goal is to develop scalable FPGA-based hardware with the flexibility to be used in a wide range of radio signal processing applications. MUSTANG-2 is the first on-sky instrument to use microwave SQUID multiplexing and is available as a shared-risk/PI instrument on the GBT. In MUSTANG-2’s first season 7 separate proposals were awarded a total of 230 hours of telescope time.

Phase division multiplexed EIT for enhanced temporal resolution.

PubMed

Dowrick, T; Holder, D

2018-03-29

The most commonly used EIT paradigm (time division multiplexing) limits the temporal resolution of impedance images due to the need to switch between injection electrodes. Advances have previously been made using frequency division multiplexing (FDM) to increase temporal resolution, but in cases where a fixed range of frequencies is available, such as imaging fast neural activity, an upper limit is placed on the total number of simultaneous injections. The use of phase division multiplexing (PDM) where multiple out of phase signals can be injected at each frequency is investigated to increase temporal resolution. TDM, FDM and PDM were compared in head tank experiments, to compare transfer impedance measurements and spatial resolution between the three techniques. A resistor phantom paradigm was established to investigate the imaging of one-off impedance changes, of magnitude 1% and with durations as low as 500 µs (similar to those seen in nerve bundles), using both PDM and TDM approaches. In head tank experiments, a strong correlation (r  >  0.85 and p  <  0.001) was present between the three sets of measured transfer impedances, and no statistically significant difference was found in reconstructed image quality. PDM was able to image impedance changes down to 500 µs in the phantom experiments, while the minimum duration imaged using TDM was 5 ms. PDM offers a possible solution to the imaging of fast moving impedance changes (such as in nerves), where the use of triggering or coherent averaging is not possible. The temporal resolution presents an order of magnitude improvement of the TDM approach, and the approach addresses the limited spatial resolution of FDM by increasing the number of simultaneous EIT injections.

High Speed and High Spatial Density Parameter Measurement Using Fiber Optic Sensing Technology

NASA Technical Reports Server (NTRS)

Richards, William Lance (Inventor); Piazza, Anthony (Inventor); Parker, Allen R. Jr. (Inventor); Hamory, Philip J (Inventor); Chan, Hon Man (Inventor)

2017-01-01

The present invention is an improved fiber optic sensing system (FOSS) having the ability to provide both high spatial resolution and high frequency strain measurements. The inventive hybrid FOSS fiber combines sensors from high acquisition speed and low spatial resolution Wavelength-Division Multiplexing (WDM) systems and from low acquisition speed and high spatial resolution Optical Frequency Domain Reflection (OFDR) systems. Two unique light sources utilizing different wavelengths are coupled with the hybrid FOSS fiber to generate reflected data from both the WDM sensors and OFDR sensors operating on a single fiber optic cable without incurring interference from one another. The two data sets are then de-multiplexed for analysis, optionally with conventionally-available WDM and OFDR system analyzers.

Orthogonal frequency-division multiplexing access (OFDMA) based wireless visible light communication (VLC) system

NASA Astrophysics Data System (ADS)

Sung, Jiun-Yu; Yeh, Chien-Hung; Chow, Chi-Wai; Lin, Wan-Feng; Liu, Yang

2015-11-01

An orthogonal frequency-division multiplexing access (OFDMA) based visible light communication (VLC) system is proposed in this paper. The architecture of the proposed system is divided into several VLC cells, which is defined in this paper. The deployment and upgrade of the system involve only simple combination of the VLC cells. Hence it is economically advantageous. To guarantee smooth communication, nearly equal data rate is provided at every location within the system with no concern on the system scale. The user location monitor strategy is also discussed to solve the region division issues. The characteristics of the proposed system are analyzed in detail in this paper. A one-dimensional experiment was demonstrated with 13.6 Mb/s data rate.

FIBER AND INTEGRATED OPTICS: Multiplexed optical-fiber sensors with autodyne detection

NASA Astrophysics Data System (ADS)

Potapov, V. T.; Mamedov, A. M.; Shatalin, S. V.; Yushkaĭtis, R. V.

1993-09-01

A method is proposed for multiplexing optical-fiber interference sensors. The method involves autodyne reception of frequency-modulated radiation reflected back to the laser. The response of a He-Ne laser with a linearly varying generation frequency to radiation reflected back from a single-mode fiber is studied. The spectrum of beats caused in the laser radiation by the reflection is shown to be governed by the distribution of reflectors along the fiber. The phases of the spectral components contain information about the phase shift of the reflected optical signal. A hydrophone array with a sensitivity of 30 μrad/Hz1/2 is described. A distributed temperature sensor with a spatial resolution of 1 m is also described.

Designing Two-Layer Optical Networks with Statistical Multiplexing

NASA Astrophysics Data System (ADS)

Addis, B.; Capone, A.; Carello, G.; Malucelli, F.; Fumagalli, M.; Pedrin Elli, E.

The possibility of adding multi-protocol label switching (MPLS) support to transport networks is considered an important opportunity by telecom carriers that want to add packet services and applications to their networks. However, the question that arises is whether it is suitable to have MPLS nodes just at the edge of the network to collect packet traffic from users, or also to introduce MPLS facilities on a subset of the core nodes in order to exploit packet switching flexibility and multiplexing, thus providing induction of a better bandwidth allocation. In this article, we address this complex decisional problem with the support of a mathematical programming approach. We consider two-layer networks where MPLS is overlaid on top of transport networks-synchronous digital hierarchy (SDH) or wavelength division multiplexing (WDM)-depending on the required link speed. The discussions' decisions take into account the trade-off between the cost of adding MPLS support in the core nodes and the savings in the link bandwidth allocation due to the statistical multiplexing and the traffic grooming effects induced by MPLS nodes. The traffic matrix specifies for each point-to-point request a pair of values: a mean traffic value and an additional one. Using this traffic model, the effect of statistical multiplexing on a link allows the allocation of a capacity equal to the sum of all the mean values of the traffic demands routed on the link and only the highest additional one. The proposed approach is suitable to solve real instances in reasonable time.

Tests of the standard (30 hz) NCER FM multiplex telemetry system, augmented by two timing channels and a compensation reference signal, used to record multiplexed seismic network data on magnetic tape

USGS Publications Warehouse

Eaton, Jerry P.

1976-01-01

The application of subtractive compensation to USGS seismic magnetic tape recording and playback systems was examined in a recent USGS Open-file report (1). It was found, for the standard (30 Hz) NCER multiplex system, that subtractive compensation utilizing a 4688 Hz reference signal multiplexed onto each data track was more effective than that utilizing a 3125 Hz reference signal recorded separately on a different track. Moreover, it was found that the portion of the spectrum between the uppermost data channel (3060 Hz + or - 125 Hz) and the compensation reference signal (4688 Hz) could be used to record an additional timing signal, with a center frequency of 3700 Hz and a broader playback bandwidth (ca 0 to 100 Hz) than that of the standard data channels. Accordingly, for the tests described in that report, the standard 8-datachannel multiplex system was augmented by one additional timing channel with a center frequency of 3700 Hz. The 3700 Hz discriminator used in those tests was not successfully set up to utilize subtractive compensation; so its output from a tape playback was quite noisy. Subsequently, further tests have been carried out on the application of subtractive compensation to a 4-channel broad-band multiplex system and to the standard multiplex system, both recorded on field tape recorders with relatively poor tape speed control (2), (3). In the course of these experiments, it was discovered that two separate timing channe1s, not just one, can be inserted between the uppermost data channel and the compensation reference signal, Furthermore, it was possible to adjust the discriminators used to playback these timing channels so that they profited significantly from subtractive compensation even though the playback bandwidth was 0 to 100 Hz (for short rise times of square wave timing signals). The advantages of recording two timing signals on each data track include: 1) one standard time signal to be used for critical timing, e.g. IRIG E, can be recorded with the data on each track, eliminating any problem that might arise from tape head misalignment if the timing base were recorded on a separate track from the data signals being timed, 2) other essential timing signals e.g. WWVB and IRIG C, can each be recorded on several tracks, to insure more reliable recording through redundancy, without displacing data from standard data channels, 3) the broader playback bandwidth of the special timing channels reproduces the sharp-edged timing codes with much less distortion than is obtained from the standard data channels. In order to implement subtractive compensation with the 4688 Hz multiplexed signal and to record timing signals on the proposed special timing channels, it was necessary to design and build a signal generator/multiplexer unit. The functions of this unit are: 1) generate a stable (x-tal controlled) compensation reference frequency (4688 Hz), 2) generate two timing channel subcarriers (3500 Hz and 3950 Hz) and provide for their modulation by appropriate timing signals, 3) separately, for each of th~14 tape tracks, adjust the relative levels of the timing and compensation subcarriers and multiplex them, at the appropriate level, with the incoming multiplexed data signals for introduction to the tape system direct record amplifiers. These units will be described in detail by Gray Jensen, who designed and built them, as well as being shown diagrammatically in this report. This report continues the work described in reference (1), and it should be read as a supplement to that report rather than as an independent effort. It introduces changes in the multiplex system and test circuits employed in the tests and then repeats the tests from the earlier report that are required to illustrate the characteristics of the modified system.

Parallel phase-shifting self-interference digital holography with faithful reconstruction using compressive sensing

NASA Astrophysics Data System (ADS)

Wan, Yuhong; Man, Tianlong; Wu, Fan; Kim, Myung K.; Wang, Dayong

2016-11-01

We present a new self-interference digital holographic approach that allows single-shot capturing three-dimensional intensity distribution of the spatially incoherent objects. The Fresnel incoherent correlation holographic microscopy is combined with parallel phase-shifting technique to instantaneously obtain spatially multiplexed phase-shifting holograms. The compressive-sensing-based reconstruction algorithm is implemented to reconstruct the original object from the under sampled demultiplexed holograms. The scheme is verified with simulations. The validity of the proposed method is experimentally demonstrated in an indirectly way by simulating the use of specific parallel phase-shifting recording device.

A frequency standard via spectrum analysis and direct digital synthesis

NASA Astrophysics Data System (ADS)

Li, Dawei; Shi, Daiting; Hu, Ermeng; Wang, Yigen; Tian, Lu; Zhao, Jianye; Wang, Zhong

2014-11-01

We demonstrated a frequency standard based on a detuned coherent population beating phenomenon. In this phenomenon, the beat frequency of the radio frequency for laser modulation and the hyperfine splitting can be obtained by digital signal processing technology. After analyzing the spectrum of the beat frequency, the fluctuation information is obtained and applied to compensate for the frequency shift to generate the standard frequency by the digital synthesis method. Frequency instability of 2.6 × 1012 at 1000 s is observed in our preliminary experiment. By eliminating the phase-locking loop, the method will enable us to achieve a full-digital frequency standard with remarkable stability.

Design and performance of a custom ASIC digitizer for wire chamber readout in 65 nm CMOS technology

NASA Astrophysics Data System (ADS)

Lee, M. J.; Brown, D. N.; Chang, J. K.; Ding, D.; Gnani, D.; Grace, C. R.; Jones, J. A.; Kolomensky, Y. G.; von der Lippe, H.; Mcvittie, P. J.; Stettler, M. W.; Walder, J.-P.

2015-06-01

We present the design and performance of a prototype ASIC digitizer for integrated wire chamber readout, implemented in 65 nm commercial CMOS technology. Each channel of the 4-channel prototype is composed of two 16-bit Time-to-Digital Converters (TDCs), one 8-bit Analog-to-Digital Converter (ADC), a front-end preamplifier and shaper, plus digital and analog buffers that support a variety of digitization chains. The prototype has a multiplexed digital backend that executes a state machine, distributes control and timing signals, and buffers data for serial output. Laboratory bench tests measure the absolute TDC resolution between 74 ps and 480 ps, growing with the absolute delay, and a relative time resolution of 19 ps. Resolution outliers due to cross-talk between clock signals and supply or reference voltages are seen. After calibration, the ADC displays good linearity and noise performance, with an effective number of bits of 6.9. Under normal operating conditions the circuit consumes 32 mW per channel. Potential design improvements to address the resolution drift and tails are discussed.

Acceleration Recorder and Playback Module

NASA Technical Reports Server (NTRS)

Bozeman, Richard J., Jr. (Inventor)

1996-01-01

The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

Acceleration recorder and playback module

NASA Astrophysics Data System (ADS)

Bozeman, Richard J., Jr.

1994-11-01

The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

Acceleration recorder and playback module

NASA Astrophysics Data System (ADS)

Bozeman, Richard J., Jr.

1992-09-01

The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

Acceleration recorder and playback module

NASA Technical Reports Server (NTRS)

Bozeman, Richard J., Jr. (Inventor)

1994-01-01

The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

Mode coupling at connectors in mode-division multiplexed transmission over few-mode fiber.

PubMed

Vuong, Jordi; Ramantanis, Petros; Frignac, Yann; Salsi, Massimiliano; Genevaux, Philippe; Bendimerad, Djalal F; Charlet, Gabriel

2015-01-26

In mode-division multiplexed (MDM) transmission systems, mode coupling is responsible for inter-modal crosstalk. We consider the transmission of modulated signals over a few-mode fiber (FMF) having low mode coupling and large differential mode group delay in the presence of a non-ideal fiber connection responsible for extra mode coupling. In this context, we first analytically derive the coupling matrix of the multimode connector and we numerically study the dependence of the matrix coefficients as a function of the butt-joint connection characteristics. The numerical results are then validated through an experiment with a five-mode setup. Finally, through numerical simulations, we assess the impact of the connector on the signal quality investigating different receiver digital signal processing (DSP) schemes.

Superwide-angle coverage code-multiplexed optical scanner.

PubMed

Riza, Nabeel A; Arain, Muzammil A

2004-05-01

A superwide-angle coverage code-multiplexed optical scanner is presented that has the potential to provide 4 pi-sr coverage. As a proof-of-concept experiment, an angular scan range of 288 degrees for six randomly distributed beams is demonstrated. The proposed scanner achieves its superwide coverage by exploiting a combination of phase-encoded transmission and reflection holography within an in-line hologram recording-retrieval geometry. The basic scanner unit consists of one phase-only digital mode spatial light modulator for code entry (i.e., beam scan control) and a holographic material from which we obtained what we believe is the first-of-a-kind extremely wide coverage, low component count, high speed (e.g., microsecond domain), and large aperture (e.g., > 1-cm diameter) scanner.

Finite-dimensional modeling of network-induced delays for real-time control systems

NASA Technical Reports Server (NTRS)

Ray, Asok; Halevi, Yoram

1988-01-01

In integrated control systems (ICS), a feedback loop is closed by the common communication channel, which multiplexes digital data from the sensor to the controller and from the controller to the actuator along with the data traffic from other control loops and management functions. Due to asynchronous time-division multiplexing in the network access protocols, time-varying delays are introduced in the control loop, which degrade the system dynamic performance and are a potential source of instability. The delayed control system is represented by a finite-dimensional, time-varying, discrete-time model which is less complex than the existing continuous-time models for time-varying delays; this approach allows for simpler schemes for analysis and simulation of the ICS.

Systems Issues Pertaining to Holographic Optical Data Storage in Thick Bacteriorhodopsin Films

NASA Technical Reports Server (NTRS)

Downie, John D.; Timucin, Dogan A.; Gary, Charles K.; Oezcan, Meric; Smithey, Daniel T.; Crew, Marshall; Lau, Sonie (Technical Monitor)

1998-01-01

The optical data storage capacity and raw bit-error-rate achievable with thick photochromic bacteriorhodopsin (BR) films are investigated for sequential recording and read- out of angularly- and shift-multiplexed digital holograms inside a thick blue-membrane D85N BR film. We address the determination of an exposure schedule that produces equal diffraction efficiencies among each of the multiplexed holograms. This exposure schedule is determined by numerical simulations of the holographic recording process within the BR material, and maximizes the total grating strength. We also experimentally measure the shift selectivity and compare the results to theoretical predictions. Finally, we evaluate the bit-error-rate of a single hologram, and of multiple holograms stored within the film.

A Novel Multiplex HRM Assay to Detect Clopidogrel Resistance.

PubMed

Zhang, Lichen; Ma, Xiaowei; You, Guoling; Zhang, Xiaoqing; Fu, Qihua

2017-11-22

Clopidogrel is an antiplatelet medicine used to prevent blood clots in patients who have had a heart attack, stroke, or other symptoms. Variability in the clinical response to clopidogrel treatment has been attributed to genetic factors. In particular, five SNPs of rs4244285, rs4986893, rs12248560, rs662 and rs1045642 have been associated with resistance to clopidogrel therapy in Chinese population. This work involves the development of a multiplex high-resolution melting (HRM) assay to genotype all five of these loci in 2 tubes. Amplicons corresponding to distinct SNPs in a common tube were designed with the aid of uMelt prediction software to have different melting temperatures Tm by addition of a GC-rich tail to the 5' end of the certain primers. Two kinds of commercial methods, Digital Fluorescence Molecular Hybridization (DFMH) and Sanger sequencing, were used as a control. Three hundred sixteen DFMH pretested samples from consecutive acute coronary syndrome patients were used for a blinded study of multiplex HRM. The sensitivity of HRM was 100% and the specificity was 99.93% reflecting detection of variants other than the known resistance SNPs. Multiplex HRM is an effective closed-tube, highly accurate, fast, and inexpensive method for genotyping the 5 clopidogrel resistance associated SNPs.

Angular multiplexing holograms of four images recorded on photopolymer films with recording-film-thickness-dependent holographic characteristics

NASA Astrophysics Data System (ADS)

Osabe, Keiichi; Kawai, Kotaro

2017-03-01

In this study, angular multiplexing hologram recording photopolymer films were studied experimentally. The films contained acrylamide as a monomer, eosin Y as a sensitizer, and triethanolamine as a promoter in a polyvinyl alcohol matrix. In order to determine the appropriate thickness of the photopolymer films for angular multiplexing, photopolymer films with thicknesses of 29-503 μm were exposed to two intersecting beams of a YVO laser at a wavelength of 532 nm to form a holographic grating with a spatial frequency of 653 line/mm. The diffraction efficiencies as a function of the incident angle of reconstruction were measured. A narrow angular bandwidth and high diffraction efficiency are required for angular multiplexing; hence, we define the Q value, which is the diffraction efficiency divided by half the bandwidth. The Q value of the films depended on the thickness of the films, and was calculated based on the measured diffraction efficiencies. The Q value of a 297-μm-thick film was the highest of the all films. Therefore, the angular multiplexing experiments were conducted using 300-μm-thick films. In the angular multiplexing experiments, the object beam transmitted by a square aperture was focused by a Fourier transform lens and interfered with a reference beam. The maximum order of angular multiplexing was four. The signal intensity that corresponds to the squared-aperture transmission and the noise intensity that corresponds to transmission without the square aperture were measured. The signal intensities decreased as the order of angular multiplexing increased, and the noise intensities were not dependent on the order of angular multiplexing.

Clinical utility of circulating tumor DNA for molecular assessment in pancreatic cancer.

PubMed

Takai, Erina; Totoki, Yasushi; Nakamura, Hiromi; Morizane, Chigusa; Nara, Satoshi; Hama, Natsuko; Suzuki, Masami; Furukawa, Eisaku; Kato, Mamoru; Hayashi, Hideyuki; Kohno, Takashi; Ueno, Hideki; Shimada, Kazuaki; Okusaka, Takuji; Nakagama, Hitoshi; Shibata, Tatsuhiro; Yachida, Shinichi

2015-12-16

Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. The genomic landscape of the PDAC genome features four frequently mutated genes (KRAS, CDKN2A, TP53, and SMAD4) and dozens of candidate driver genes altered at low frequency, including potential clinical targets. Circulating cell-free DNA (cfDNA) is a promising resource to detect and monitor molecular characteristics of tumors. In the present study, we determined the mutational status of KRAS in plasma cfDNA using multiplex picoliter-droplet digital PCR in 259 patients with PDAC. We constructed a novel modified SureSelect-KAPA-Illumina platform and an original panel of 60 genes. We then performed targeted deep sequencing of cfDNA and matched germline DNA samples in 48 patients who had ≥1% mutant allele frequencies of KRAS in plasma cfDNA. Importantly, potentially targetable somatic mutations were identified in 14 of 48 patients (29.2%) examined by targeted deep sequencing of cfDNA. We also analyzed somatic copy number alterations based on the targeted sequencing data using our in-house algorithm, and potentially targetable amplifications were detected. Assessment of mutations and copy number alterations in plasma cfDNA may provide a prognostic and diagnostic tool to assist decisions regarding optimal therapeutic strategies for PDAC patients.

Parallel multiplex laser feedback interferometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Song; Tan, Yidong; Zhang, Shulian, E-mail: zsl-dpi@mail.tsinghua.edu.cn

2013-12-15

We present a parallel multiplex laser feedback interferometer based on spatial multiplexing which avoids the signal crosstalk in the former feedback interferometer. The interferometer outputs two close parallel laser beams, whose frequencies are shifted by two acousto-optic modulators by 2Ω simultaneously. A static reference mirror is inserted into one of the optical paths as the reference optical path. The other beam impinges on the target as the measurement optical path. Phase variations of the two feedback laser beams are simultaneously measured through heterodyne demodulation with two different detectors. Their subtraction accurately reflects the target displacement. Under typical room conditions, experimentalmore » results show a resolution of 1.6 nm and accuracy of 7.8 nm within the range of 100 μm.« less

Experimental study on the statistic characteristics of a 3x3 RF MIMO channel over a single conventional multimode fiber.

PubMed

Lei, Yi; Li, Jianqiang; Wu, Rui; Fan, Yuting; Fu, Songnian; Yin, Feifei; Dai, Yitang; Xu, Kun

2017-06-01

Based on the observed random fluctuation phenomenon of speckle pattern across multimode fiber (MMF) facet and received optical power distribution across three output ports, we experimentally investigate the statistic characteristics of a 3×3 radio frequency multiple-input multiple-output (MIMO) channel enabled by mode division multiplexing in a conventional 50 µm MMF using non-mode-selective three-dimensional waveguide photonic lanterns as mode multiplexer and demultiplexer. The impacts of mode coupling on the MIMO channel coefficients, channel matrix, and channel capacity have been analyzed over different fiber lengths. The results indicate that spatial multiplexing benefits from the greater fiber length with stronger mode coupling, despite a higher optical loss.

Invited Article: Generation of one-million-mode continuous-variable cluster state by unlimited time-domain multiplexing

NASA Astrophysics Data System (ADS)

Yoshikawa, Jun-ichi; Yokoyama, Shota; Kaji, Toshiyuki; Sornphiphatphong, Chanond; Shiozawa, Yu; Makino, Kenzo; Furusawa, Akira

2016-09-01

In recent quantum optical continuous-variable experiments, the number of fully inseparable light modes has drastically increased by introducing a multiplexing scheme either in the time domain or in the frequency domain. Here, modifying the time-domain multiplexing experiment reported in the work of Yokoyama et al. [Nat. Photonics 7, 982 (2013)], we demonstrate the successive generation of fully inseparable light modes for more than one million modes. The resulting multi-mode state is useful as a dual-rail continuous variable cluster state. We circumvent the previous problem of optical phase drifts, which has limited the number of fully inseparable light modes to around ten thousands, by continuous feedback control of the optical system.

Parallel implementation of all-digital timing recovery for high-speed and real-time optical coherent receivers.

PubMed

Zhou, Xian; Chen, Xue

2011-05-09

The digital coherent receivers combine coherent detection with digital signal processing (DSP) to compensate for transmission impairments, and therefore are a promising candidate for future high-speed optical transmission system. However, the maximum symbol rate supported by such real-time receivers is limited by the processing rate of hardware. In order to cope with this difficulty, the parallel processing algorithms is imperative. In this paper, we propose a novel parallel digital timing recovery loop (PDTRL) based on our previous work. Furthermore, for increasing the dynamic dispersion tolerance range of receivers, we embed a parallel adaptive equalizer in the PDTRL. This parallel joint scheme (PJS) can be used to complete synchronization, equalization and polarization de-multiplexing simultaneously. Finally, we demonstrate that PDTRL and PJS allow the hardware to process 112 Gbit/s POLMUX-DQPSK signal at the hundreds MHz range. © 2011 Optical Society of America

Three-color crystal digital PCR.

PubMed

Madic, J; Zocevic, A; Senlis, V; Fradet, E; Andre, B; Muller, S; Dangla, R; Droniou, M E

2016-12-01

Digital PCR is an exciting new field for molecular analysis, allowing unprecedented precision in the quantification of nucleic acids, as well as the fine discrimination of rare molecular events in complex samples. We here present a novel technology for digital PCR, Crystal Digital PCR™, which relies on the use of a single chip to partition samples into 2D droplet arrays, which are then subjected to thermal cycling and finally read using a three-color fluorescence scanning device. This novel technology thus allows three-color multiplexing, which entails a different approach to data analysis. In the present publication, we present this innovative workflow, which is both fast and user-friendly, and discuss associated data analysis issue, such as fluorescence spillover compensation and data representation. Lastly, we also present proof-of-concept of this three-color detection system, using a quadriplex assay for the detection of EGFR mutations L858R, L861Q and T790M.

Allele frequency distribution for 21 autosomal STR loci in Bhutan.

PubMed

Kraaijenbrink, Thirsa; van Driem, George L; Tshering of Gaselô, Karma; de Knijff, Peter

2007-07-20

We studied the allele frequency distribution of 21 autosomal STR loci contained in the AmpFlSTR Identifiler (Applied Biosystems), the Powerplex 16 (Promega) and the FFFL (Promega) multiplex PCR kits among 936 individuals from the Royal Kingdom of Bhutan. As such these are the first published autosomal DNA results from this country.

High-speed polarization sensitive optical frequency domain imaging with frequency multiplexing

PubMed Central

Yun, S.H.; Vakoc, B.J.; Shishkov, M.; Desjardins, A.E.; Park, B.H.; de Boer, J.F.; Tearney, G.J.; Bouma, B.E.

2009-01-01

Polarization sensitive optical coherence tomography (PS-OCT) provides a cross-sectional image of birefringence in biological samples that is complementary in many applications to the standard reflectance-based image. Recent ex vivo studies have demonstrated that birefringence mapping enables the characterization of collagen and smooth muscle concentration and distribution in vascular tissues. Instruments capable of applying these measurements percutaneously in vivo may provide new insights into coronary atherosclerosis and acute myocardial infarction. We have developed a polarization sensitive optical frequency domain imaging (PS-OFDI) system that enables high-speed intravascular birefringence imaging through a fiber-optic catheter. The novel design of this system utilizes frequency multiplexing to simultaneously measure reflectance of two incident polarization states, overcoming concerns regarding temporal variations of the catheter fiber birefringence and spatial variations in the birefringence of the sample. We demonstrate circular cross-sectional birefringence imaging of a human coronary artery ex vivo through a flexible fiber-optic catheter with an A-line rate of 62 kHz and a ranging depth of 6.2 mm. PMID:18542183

Architecture and settings optimization procedure of a TES frequency domain multiplexed readout firmware

NASA Astrophysics Data System (ADS)

Clenet, A.; Ravera, L.; Bertrand, B.; den Hartog, R.; Jackson, B.; van Leeuwen, B.-J.; van Loon, D.; Parot, Y.; Pointecouteau, E.; Sournac, A.

2014-11-01

IRAP is developing the readout electronics of the SPICA-SAFARI's TES bolometer arrays. Based on the frequency domain multiplexing technique the readout electronics provides the AC-signals to voltage-bias the detectors; it demodulates the data; and it computes a feedback to linearize the detection chain. The feedback is computed with a specific technique, so called baseband feedback (BBFB) which ensures that the loop is stable even with long propagation and processing delays (i.e. several μ s) and with fast signals (i.e. frequency carriers of the order of 5 MHz). To optimize the power consumption we took advantage of the reduced science signal bandwidth to decouple the signal sampling frequency and the data processing rate. This technique allowed a reduction of the power consumption of the circuit by a factor of 10. Beyond the firmware architecture the optimization of the instrument concerns the characterization routines and the definition of the optimal parameters. Indeed, to operate an array TES one has to properly define about 21000 parameters. We defined a set of procedures to automatically characterize these parameters and find out the optimal settings.

A code-aided carrier synchronization algorithm based on improved nonbinary low-density parity-check codes

NASA Astrophysics Data System (ADS)

Bai, Cheng-lin; Cheng, Zhi-hui

2016-09-01

In order to further improve the carrier synchronization estimation range and accuracy at low signal-to-noise ratio ( SNR), this paper proposes a code-aided carrier synchronization algorithm based on improved nonbinary low-density parity-check (NB-LDPC) codes to study the polarization-division-multiplexing coherent optical orthogonal frequency division multiplexing (PDM-CO-OFDM) system performance in the cases of quadrature phase shift keying (QPSK) and 16 quadrature amplitude modulation (16-QAM) modes. The simulation results indicate that this algorithm can enlarge frequency and phase offset estimation ranges and enhance accuracy of the system greatly, and the bit error rate ( BER) performance of the system is improved effectively compared with that of the system employing traditional NB-LDPC code-aided carrier synchronization algorithm.

A channel estimation scheme for MIMO-OFDM systems

NASA Astrophysics Data System (ADS)

He, Chunlong; Tian, Chu; Li, Xingquan; Zhang, Ce; Zhang, Shiqi; Liu, Chaowen

2017-08-01

In view of the contradiction of the time-domain least squares (LS) channel estimation performance and the practical realization complexity, a reduced complexity channel estimation method for multiple input multiple output-orthogonal frequency division multiplexing (MIMO-OFDM) based on pilot is obtained. This approach can transform the complexity of MIMO-OFDM channel estimation problem into a simple single input single output-orthogonal frequency division multiplexing (SISO-OFDM) channel estimation problem and therefore there is no need for large matrix pseudo-inverse, which greatly reduces the complexity of algorithms. Simulation results show that the bit error rate (BER) performance of the obtained method with time orthogonal training sequences and linear minimum mean square error (LMMSE) criteria is better than that of time-domain LS estimator and nearly optimal performance.

Receiver IQ mismatch estimation in PDM CO-OFDM system using training symbol

NASA Astrophysics Data System (ADS)

Peng, Dandan; Ma, Xiurong; Yao, Xin; Zhang, Haoyuan

2017-07-01

Receiver in-phase/quadrature (IQ) mismatch is hard to mitigate at the receiver via using conventional method in polarization division multiplexed (PDM) coherent optical orthogonal frequency division multiplexing (CO-OFDM) system. In this paper, a novel training symbol structure is proposed to estimate IQ mismatch and channel distortion. Combined this structure with Gram Schmidt orthogonalization procedure (GSOP) algorithm, we can get lower bit error rate (BER). Meanwhile, based on this structure one estimation method is deduced in frequency domain which can achieve the estimation of IQ mismatch and channel distortion independently and improve the system performance obviously. Numerical simulation shows that the proposed two methods have better performance than compared method at 100 Gb/s after 480 km fiber transmission. Besides, the calculation complexity is also analyzed.

Detection of Neisseria meningitidis in cerebrospinal fluid using a multiplex PCR and the Luminex detection technology.

PubMed

Møller, Jens Kjølseth

2012-01-01

Rapid clinical and laboratory diagnoses are the foundation for a successful management of serious infections with Neisseria meningitidis. A species-specific multiplex polymerase chain reaction (PCR) coupled with fluidic microarrays using microbeads (the Luminex xMAP™ Technology) can detect pathogens most frequently found in the cerebrospinal fluid of patients. The Luminex suspension array system uniquely combines flow cytometry, microspheres, laser technology, digital signal processing, and traditional chemistry. In this method, the reaction is carried out in one vessel, in which distinctly color-coded bead sets, each conjugated with a different specific nucleic acid reactant, are hybridized with the PCR products, and a reporter molecule is used to quantify the interaction. The flow-based Luminex array reader identifies each reaction (bead set) after excitation by a red classification laser. Reporter signals from each reaction are simultaneously quantified by fluorescence generated by a green reporter laser. This nonculture, multiplex assay may prove to be an important tool for optimal laboratory diagnosis, not only of meningococcal meningitis, but also of meningitis caused by other bacterial or viral pathogens.

Multiplexed phase-space imaging for 3D fluorescence microscopy.

PubMed

Liu, Hsiou-Yuan; Zhong, Jingshan; Waller, Laura

2017-06-26

Optical phase-space functions describe spatial and angular information simultaneously; examples of optical phase-space functions include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration removal and 3D reconstruction. High-resolution capture of 4D phase-space datasets is, however, challenging. Previous scanning approaches are slow, light inefficient and do not achieve diffraction-limited resolution. Here, we propose a multiplexed method that solves these problems. We use a spatial light modulator (SLM) in the pupil plane of a microscope in order to sequentially pattern multiplexed coded apertures while capturing images in real space. Then, we reconstruct the 3D fluorescence distribution of our sample by solving an inverse problem via regularized least squares with a proximal accelerated gradient descent solver. We experimentally reconstruct a 101 Megavoxel 3D volume (1010×510×500µm with NA 0.4), demonstrating improved acquisition time, light throughput and resolution compared to scanning aperture methods. Our flexible patterning scheme further allows sparsity in the sample to be exploited for reduced data capture.

Dual-channel-coded microbeads for multiplexed detection of biomolecules using assembling of quantum dots and element coding nanoparticles.

PubMed

Lu, Bangrong; He, Qinghua; He, Yonghong; Chen, Xuejing; Feng, Guangxia; Liu, Siyu; Ji, Yanhong

2018-09-18

To achieve the dual-channel (analog and digital) encoding, microbeads assembled with quantum dots (QDs) and element coding nanoparticles (ECNPs) have been prepared. Dual-spectra, including fluorescence generated from quantum dots (QDs) and laser induced breakdown spectrum obtained from the plasma of ECNPs, including AgO, MgO and ZnO nanoparticles, has been adopted to provide more encoding amounts and more accurate dual recognition for encoded microbeads in multiplexed utilization. The experimental results demonstrate that the single microbead can be decoded in two optical channels. Multiplexed analysis and contrast adsorption experiment of anti-IgG verified the availability and specificity of dual-channel-coded microbeads in bioanalysis. In gradient detection of anti-IgG, we obtained the linear concentration response to target biomolecules from 3.125 × 10 -10  M to 1 × 10 -8  M, and the limit of detection was calculated to be 2.91 × 10 -11  M. Copyright © 2018 Elsevier B.V. All rights reserved.

Multiplexed Cassegrain Reflector Antenna for Simultaneous Generation of Three Orbital Angular Momentum (OAM) Modes.

PubMed

Byun, Woo Jin; Kim, Kwang Seon; Kim, Bong Su; Lee, Young Seung; Song, Myung Sun; Choi, Hyung Do; Cho, Yong Heui

2016-06-02

A multiplexed Cassegrain reflector antenna with a 2 × 2 open-ended rectangular waveguide (OERW) matrix feed and an orbital angular momentum (OAM) mode mux is proposed for the simultaneous generation of three OAM modes (l = 0, ±1). The OAM mode mux (OMM) was designed using sequential combinations of quadrature hybrids, crossovers, and phase shifters to multiplex and demultiplex three OAM modes at the same time. The 2 × 2 OERW matrix feed and the OMM were separately measured and their performances were verified according to proposed theories. A near-field antenna measurement for a multiplexed Cassegrain reflector antenna was conducted to obtain the far-field magnitude and phase patterns around polar elevation angle θ and azimuthal angle ϕ, thus confirming that our antenna can produce three OAM modes simultaneously. We also measured the communication link characteristics of two identical multiplexed antennas. The measurement results show that the channel isolation of three OAM modes is more than 12.7 [dB] and 17 [dB] for fixed and compensated receiver positions, respectively, indicating that the proposed antenna system can be used for independent communication links with the same frequency and polarisation.

Thirtyfold multiplex genotyping of the p53 gene using solid phase capturable dideoxynucleotides and mass spectrometry.

PubMed

Kim, Sobin; Ulz, Michael E; Nguyen, Tuan; Li, Chi-Ming; Sato, Takaaki; Tycko, Benjamin; Ju, Jingyue

2004-05-01

A mass spectrometry (MS) based multiplex genotyping method using solid phase capturable (SPC) dideoxynucleotides and single base extension (SBE), named the SPC-SBE, has been developed for mutation detection. We report here the simultaneous genotyping of 30 potential point mutation sites in exons 5, 7, and 8 of the human p53 gene in one tube using the SPC-SBE method. The 30 mutation sites, including the most frequently mutated p53 codons, were chosen to explore the high multiplexing scope of the SPC-SBE method. Thirty primers specific to each potential mutation site were designed to yield SBE products with sufficient mass differences. This was achieved by tuning the mass of some primers using modified nucleotides. Genomic DNA was amplified by multiplex PCR to produce amplicons of the three p53 exons. The 30 primers were combined with the PCR products and biotinylated dideoxynucleotides for SBE to generate 3'-biotinylated extension DNA products. These products were then captured by streptavidin-coated magnetic beads, while the unextended primers and other components in the reaction were washed away. The pure extension DNA products were subsequently released from the solid phase and analyzed with MS. We simultaneously genotyped 30 potential mutation sites in the p53 gene from Wilms' tumor, head and neck tumor, and colorectal tumor. Both homozygous and heterozygous genotypes were accurately determined with digital resolution. This is the highest level of multiplex genotyping reported thus far using MS, indicating that the approach might be applicable to screening a repertoire of genotypes in candidate genes as potential disease markers.

An all-digital receiver for satellite audio broadcasting signals using trellis coded quasi-orthogonal code-division multiplexing

NASA Astrophysics Data System (ADS)

Braun, Walter; Eglin, Peter; Abello, Ricard

1993-02-01

Spread Spectrum Code Division Multiplex is an attractive scheme for the transmission of multiple signals over a satellite transponder. By using orthogonal or quasi-orthogonal spreading codes the interference between the users can be virtually eliminated. However, the acquisition and tracking of the spreading code phase can not take advantage of the code orthogonality since sequential acquisition and Delay-Locked loop tracking depend on correlation with code phases other than the optimal despreading phase. Hence, synchronization is a critical issue in such a system. A demonstration hardware for the verification of the orthogonal CDM synchronization and data transmission concept is being designed and implemented. The system concept, the synchronization scheme, and the implementation are described. The performance of the system is discussed based on computer simulations.

Wavelength division multiplexing of chaotic secure and fiber-optic communications.

PubMed

Zhang, Jian-Zhong; Wang, An-Bang; Wang, Juan-Fen; Wang, Yun-Cai

2009-04-13

Wavelength division multiplexing (WDM) transmission of chaotic optical communication (COC) and conventional fiber-optic communication (CFOC) is numerically confirmed and analyzed. For an 80-km-long two-channel communication system, a 1-Gb/s secure message in COC channel and 10-Gb/s digital signal in CFOC channel are simultaneously achieved with 100 GHz channel spacing. Our numerical simulations demonstrate that the COC and CFOC can realize no-crosstalk transmission of 80 km when the peak power of CFOC channel is less than 8dBm. We also find that the crosstalk between COC and CFOC does not depend on channel spacing when the channel spacing exceeds 100GHz. Moreover, the crosstalk does not limit channel number by comparing the synchronization performance of COC in four- and six-channel WDM systems.

Photonic engineering for biological study

NASA Astrophysics Data System (ADS)

Wu, Fei

My dissertation focuses on designing and developing prototypes of optical tools in the laboratory that can facilitate practical medical therapies. More specifically, this dissertation examines two novel biophotonic techniques: (1) a frequency multiplexed confocal microscope with the potential to provide rational therapy of congestive heart failure (CHF), and (2) the "optical comb" with the potential to improve results of retina reattachment surgery and accelerate post surgical recovery. Next, I will discuss the background, design and initial experimental results of each study individually. Part I: The Frequency Multiplexed Confocal Microscope. To overcome the limitations of existing confocal microscope technology, this dissertation proposes a non-scanning, real-time, high resolution technique (a multi-point frequency multiplexed confocal microscope) to measure 3-D intracellular calcium ion concentration in a living cardiac myocyte. This method can be also applied to measure the intracellular sodium ion concentration, or other ions in which high quantum-yield fluorescent probes are available. The novelty of the proposed research lies in the introduction of carrier frequency multiplexing techniques which can differentiate fluorescence emitted at different spatial locations in cardiac myocyte by their modulated frequency. It therefore opens the possibility to visualize the transient dynamics of intracellular dynamics at multiple locations in cells simultaneously, which will shine a new light on our understanding of CHF. The procedure for frequency multiplexing proposed is described below. Multiple incident laser beams are focused onto different locations in an isolated rat cardiac myocyte with each beam modulated at a different carrier frequency. The fluorescence emission at each location therefore bears the same modulated frequency as the stimulation laser beam. Each fluorescence signal is sent to the photo multiplier tube (PMT) after being spatially filtered by a single mode fiber (functioning as a pinhole). Since each signal has a different carrier frequency, only one signal detector is required to collect multiple signal streams which eliminates the errors introduced by difference of multiple detectors. After taking the Fourier Transform of the collected data, multiple peaks can be found in the frequency domain. Each peak refers to a corresponding location in the sample. The temporal information of the fluorescence signal variation at each location can be obtained by demodulating the low frequency information from the carrier frequency, followed by an inverse Fourier transform. Part II: The "Optical Comb". Retinal detachment refers to separation of the inner layers of the retina from the underlying retinal pigment epithelium. It can cause degeneration of the retina and may lead to permanent vision loss if not promptly treated and hence is considered an ocular emergency. Currently, the only treatment available for retinal detachment is surgical reattachment. The idea of an "optical comb" is developed from the general working principle of the well known "optical tweezers" in the optical literature, which can pull micro-objects through the trapping force produced by a focused laser beam. If we can manage to incident the focused laser beam onto the misaligned photoreceptors and further scan it back and forth, trapping forces that produced may be able to "comb" the photoreceptors to be aligned, and thereby help with post surgery recovery. A series of experiments have been carried out to demonstrate the plausibility of this idea. First, several micro glass rods with size similar to human's photoreceptors (6 microns in diameter and 30 microns in length) were used. We observed that when the laser beam is focused close to one end of the micro rod originally laid on a glass coverslip, the rod is pulled to stand upright successfully, and we can manipulate the direction it faces by controlling its relative position to the laser beam. We are now experimenting with this combing technique with detached bovine retina samples to further verify its feasibility over live animal cells. (Abstract shortened by UMI.)

A multiplexed light-matter interface for fibre-based quantum networks

PubMed Central

Saglamyurek, Erhan; Grimau Puigibert, Marcelli; Zhou, Qiang; Giner, Lambert; Marsili, Francesco; Verma, Varun B.; Woo Nam, Sae; Oesterling, Lee; Nippa, David; Oblak, Daniel; Tittel, Wolfgang

2016-01-01

Processing and distributing quantum information using photons through fibre-optic or free-space links are essential for building future quantum networks. The scalability needed for such networks can be achieved by employing photonic quantum states that are multiplexed into time and/or frequency, and light-matter interfaces that are able to store and process such states with large time-bandwidth product and multimode capacities. Despite important progress in developing such devices, the demonstration of these capabilities using non-classical light remains challenging. Here, employing the atomic frequency comb quantum memory protocol in a cryogenically cooled erbium-doped optical fibre, we report the quantum storage of heralded single photons at a telecom-wavelength (1.53 μm) with a time-bandwidth product approaching 800. Furthermore, we demonstrate frequency-multimode storage and memory-based spectral-temporal photon manipulation. Notably, our demonstrations rely on fully integrated quantum technologies operating at telecommunication wavelengths. With improved storage efficiency, our light-matter interface may become a useful tool in future quantum networks. PMID:27046076

A multiplexed light-matter interface for fibre-based quantum networks.

PubMed

Saglamyurek, Erhan; Grimau Puigibert, Marcelli; Zhou, Qiang; Giner, Lambert; Marsili, Francesco; Verma, Varun B; Woo Nam, Sae; Oesterling, Lee; Nippa, David; Oblak, Daniel; Tittel, Wolfgang

2016-04-05

Processing and distributing quantum information using photons through fibre-optic or free-space links are essential for building future quantum networks. The scalability needed for such networks can be achieved by employing photonic quantum states that are multiplexed into time and/or frequency, and light-matter interfaces that are able to store and process such states with large time-bandwidth product and multimode capacities. Despite important progress in developing such devices, the demonstration of these capabilities using non-classical light remains challenging. Here, employing the atomic frequency comb quantum memory protocol in a cryogenically cooled erbium-doped optical fibre, we report the quantum storage of heralded single photons at a telecom-wavelength (1.53 μm) with a time-bandwidth product approaching 800. Furthermore, we demonstrate frequency-multimode storage and memory-based spectral-temporal photon manipulation. Notably, our demonstrations rely on fully integrated quantum technologies operating at telecommunication wavelengths. With improved storage efficiency, our light-matter interface may become a useful tool in future quantum networks.

Multi-wavelength lenses for terahertz surface wave.

PubMed

Wei, Minggui; Yang, Quanlong; Xu, Quan; Zhang, Xueqian; Li, Yanfeng; Gu, Jianqiang; Han, Jiaguang; Zhang, Weili

2017-10-16

Metasurface-based surface wave (SW) devices working at multi-wavelength has been continuously arousing enormous curiosity recently, especially in the terahertz community. In this work, we propose a multi-layer metasurface structure composed of metallic slit pairs to build terahertz SW devices. The slit pair has a narrow bandwidth and its response frequency can be altered by its geometric parameter, thereby suppressing the frequency crosstalk and reducing the difficulty of design. By elaborately tailoring the distribution of the slit pairs, a series of achromatic SW lenses (SWLs) working at 0.6, 0.75 and 1 THz are experimentally demonstrated by the near field scanning terahertz microscope (NSTM) system. In addition, a wavelength-division-multiplexer (WDM) is further designed and implemented, which is promising in building multiplexed devices for plasmonic circuits. The structure proposed here cannot only couple the terahertz wave from free space to SWs, but also control its propagation. Moreover, our findings demonstrate the great potential to design multi-wavelength plasmonic metasurface devices, which can be extended to microwave and visible frequencies as well.

Compressive self-interference Fresnel digital holography with faithful reconstruction

NASA Astrophysics Data System (ADS)

Wan, Yuhong; Man, Tianlong; Han, Ying; Zhou, Hongqiang; Wang, Dayong

2017-05-01

We developed compressive self-interference digital holographic approach that allows retrieving three-dimensional information of the spatially incoherent objects from single-shot captured hologram. The Fresnel incoherent correlation holography is combined with parallel phase-shifting technique to instantaneously obtain spatial-multiplexed phase-shifting holograms. The recording scheme is regarded as compressive forward sensing model, thus the compressive-sensing-based reconstruction algorithm is implemented to reconstruct the original object from the under sampled demultiplexed sub-holograms. The concept was verified by simulations and experiments with simulating use of the polarizer array. The proposed technique has great potential to be applied in 3D tracking of spatially incoherent samples.

Crosstalk mitigation using pilot assisted least square algorithm in OFDM-carrying orbital angular momentum multiplexed free-space-optical communication links.

PubMed

Sun, Tengfen; Liu, Minwen; Li, Yingchun; Wang, Min

2017-10-16

In this paper, we experimentally investigate the performance of crosstalk mitigation for 16-ary quadrature amplitude modulation orthogonal frequency division multiplexing (16QAM-OFDM) signals carrying orbital angular momentum (OAM) multiplexed free-space-optical communication (FSO) links using the pilot assisted Least Square (LS) algorithm. At the demodulating spatial light modulators (SLMs), we launch the distorted phase holograms which have the information of atmospheric turbulence obeying the modified Hill spectrum. And crosstalk can be introduced by these holograms with the experimental verification. The pilot assisted LS algorithm can efficiently improve the quality of system performance, the points of constellations get closer to the reference points and around two orders of magnitude improvement of bit-error rate (BER) is obtained.

60-GHz optical/wireless MIMO system integrated with optical subcarrier multiplexing and 2x2 wireless communication.

PubMed

Lin, Chi-Hsiang; Lin, Chun-Ting; Huang, Hou-Tzu; Zeng, Wei-Siang; Chiang, Shou-Chih; Chang, Hsi-Yu

2015-05-04

This paper proposes a 2x2 MIMO OFDM Radio-over-Fiber scheme based on optical subcarrier multiplexing and 60-GHz MIMO wireless transmission. We also schematically investigated the principle of optical subcarrier multiplexing, which is based on a dual-parallel Mach-Zehnder modulator (DP-MZM). In our simulation result, combining two MIMO OFDM signals to drive DP-MZM gives rise to the PAPR augmentation of less than 0.4 dB, which mitigates nonlinear distortion. Moreover, we applied a Levin-Campello bit-loading algorithm to compensate for the uneven frequency responses in the V-band. The resulting system achieves OFDM signal rates of 61.5-Gbits/s with BER of 10(-3) over 25-km SMF transmission followed by 3-m wireless transmission.

An all digital low data rate communication system

NASA Technical Reports Server (NTRS)

Chen, C.-H.; Fan, M.

1973-01-01

The advent of digital hardwares has made it feasible to implement many communication system components digitally. With the exception of frequency down conversion, the proposed low data rate communication system uses digital hardware completely. Although the system is designed primarily for deep space communications with large frequency uncertainty and low signal-to-noise ratio, it is also suitable for other low data rate applications with time-shared operation among a number of channels. Emphasis is placed on the fast Fourier transform receiver and the automatic frequency control via digital filtering. The speed available from the digital system allows sophisticated signal processing to reduce frequency uncertainty and to increase the signal-to-noise ratio.

Experimental demonstration of a frequency-domain Volterra series nonlinear equalizer in polarization-multiplexed transmission.

PubMed

Guiomar, Fernando P; Reis, Jacklyn D; Carena, Andrea; Bosco, Gabriella; Teixeira, António L; Pinto, Armando N

2013-01-14

Employing 100G polarization-multiplexed quaternary phase-shift keying (PM-QPSK) signals, we experimentally demonstrate a dual-polarization Volterra series nonlinear equalizer (VSNE) applied in frequency-domain, to mitigate intra-channel nonlinearities. The performance of the dual-polarization VSNE is assessed in both single-channel and in wavelength-division multiplexing (WDM) scenarios, providing direct comparisons with its single-polarization version and with the widely studied back-propagation split-step Fourier (SSF) approach. In single-channel transmission, the optimum power has been increased by about 1 dB, relatively to the single-polarization equalizers, and up to 3 dB over linear equalization, with a corresponding bit error rate (BER) reduction of up to 63% and 85%, respectively. Despite of the impact of inter-channel nonlinearities, we show that intra-channel nonlinear equalization is still able to provide approximately 1 dB improvement in the optimum power and a BER reduction of ~33%, considering a 66 GHz WDM grid. By means of simulation, we demonstrate that the performance of nonlinear equalization can be substantially enhanced if both optical and electrical filtering are optimized, enabling the VSNE technique to outperform its SSF counterpart at high input powers.

Multiplexing Technology for Acoustic Emission Monitoring of Aerospace Vehicles

NASA Technical Reports Server (NTRS)

Prosser, William; Percy, Daniel

2003-01-01

The initiation and propagation of damage mechanisms such as cracks and delaminations generate acoustic waves, which propagate through a structure. These waves can be detected and analyzed to provide the location and severity of damage as part of a structural health monitoring (SHM) system. This methodology of damage detection is commonly known as acoustic emission (AE) monitoring, and is widely used on a variety of applications on civil structures. AE has been widely considered for SHM of aerospace vehicles. Numerous successful ground and flight test demonstrations have been performed, which show the viability of the technology for damage monitoring in aerospace structures. However, one significant current limitation for application of AE techniques on aerospace vehicles is the large size, mass, and power requirements for the necessary monitoring instrumentation. To address this issue, a prototype multiplexing approach has been developed and demonstrated in this study, which reduces the amount of AE monitoring instrumentation required. Typical time division multiplexing techniques that are commonly used to monitor strain, pressure and temperature sensors are not applicable to AE monitoring because of the asynchronous and widely varying rates of AE signal occurrence. Thus, an event based multiplexing technique was developed. In the initial prototype circuit, inputs from eight sensors in a linear array were multiplexed into two data acquisition channels. The multiplexer rapidly switches, in less than one microsecond, allowing the signals from two sensors to be acquired by a digitizer. The two acquired signals are from the sensors on either side of the trigger sensor. This enables the capture of the first arrival of the waves, which cannot be accomplished with the signal from the trigger sensor. The propagation delay to the slightly more distant neighboring sensors makes this possible. The arrival time from this first arrival provides a more accurate source location determination. The multiplexer also identifies which channels are acquired by encoding TTL logic pulses onto the latter portion of the signals. This prototype system was demonstrated using pencil lead break (Hsu-Neilsen) sources on an aluminum plate. It performed as designed providing rapid low noise trigger based switching with encoded channel identification. this multiplexing approach is not limited to linear arrays, but can be easily extended to monitor sensors in planar ot three dimensional arrays. A 32 channel multiplexing system is under development that will allow arbitrary sensor placement. Another benefit of this multiplexing system is the reduction in the expense of data acquisition hardware. In addition, the reduced weight and power requirements are of extreme importance for proposed AE systems on aerospace vehicles.

Time- and frequency-resolved measurements of frequency modulation and switching of a tunable semiconductor laser

NASA Astrophysics Data System (ADS)

Kuznetsov, M.; Stone, J.; Stulz, L. W.

1991-11-01

We report measurements of intensity as a function of both time and frequency for frequency modulation and switching of a tunable semiconductor laser. Because of the uncertainty principle limitations, the measured time-frequency signal can have a complex structure and does not show the simple-minded picture of a laser spectrum whose center frequency varies in time. The observations are explained by a theory of the time-dependent spectral measurements, well known in the field of speech analysis. We discuss implications for channel switching speed and channel interference in switched, frequency-multiplexed optical networks.

Multiplexed homogeneous assays of proteolytic activity using a smartphone and quantum dots.

PubMed

Petryayeva, Eleonora; Algar, W Russ

2014-03-18

Semiconductor quantum dot (QD) bioconjugates, with their unique and highly advantageous physicochemical and optical properties, have been extensively utilized as probes for bioanalysis and continue to generate widespread interest for these applications. An important consideration for expanding the utility of QDs and making their use routine is to make assays with QDs more accessible for laboratories that do not specialize in nanomaterials. Here, we show that digital color imaging of QD photoluminescence (PL) with a smartphone camera is a viable, easily accessible readout platform for quantitative, multiplexed, and real-time bioanalyses. Red-, green-, and blue-emitting CdSeS/ZnS QDs were conjugated with peptides that were labeled with a deep-red fluorescent dye, Alexa Fluor 647, and the dark quenchers, QSY9 and QSY35, respectively, to generate Förster resonance energy transfer (FRET) pairs sensitive to proteolytic activity. Changes in QD PL caused by the activity of picomolar to nanomolar concentrations of protease were detected as changes in the red-green-blue (RGB) channel intensities in digital color images. Importantly, measurements of replicate samples made with smartphone imaging and a sophisticated fluorescence plate reader yielded the same quantitative results, including initial proteolytic rates and specificity constants. Homogeneous two-plex and three-plex assays for the activity of trypsin, chymotrypsin, and enterokinase were demonstrated with RGB imaging. Given the ubiquity of smartphones, this work largely removes any instrumental impediments to the adoption of QDs as routine tools for bioanalysis in research laboratories and is a critical step toward the use of QDs for point-of-care diagnostics. This work also adds to the growing utility of smartphones in analytical methods by enabling multiplexed fluorimetric assays within a single sample volume and across multiple samples in parallel.

Multiplex Droplet Digital PCR Quantification of Recurrent Somatic Mutations in Diffuse Large B-Cell and Follicular Lymphoma.

PubMed

Alcaide, Miguel; Yu, Stephen; Bushell, Kevin; Fornika, Daniel; Nielsen, Julie S; Nelson, Brad H; Mann, Koren K; Assouline, Sarit; Johnson, Nathalie A; Morin, Ryan D

2016-09-01

A plethora of options to detect mutations in tumor-derived DNA currently exist but each suffers limitations in analytical sensitivity, cost, or scalability. Droplet digital PCR (ddPCR) is an appealing technology for detecting the presence of specific mutations based on a priori knowledge and can be applied to tumor biopsies, including formalin-fixed paraffin embedded (FFPE) tissues. More recently, ddPCR has gained popularity in its utility in quantifying circulating tumor DNA. We have developed a suite of novel ddPCR assays for detecting recurrent mutations that are prevalent in common B-cell non-Hodgkin lymphomas (NHLs), including diffuse large B-cell lymphoma, follicular lymphoma, and lymphoplasmacytic lymphoma. These assays allowed the differentiation and counting of mutant and wild-type molecules using one single hydrolysis probe. We also implemented multiplexing that allowed the simultaneous detection of distinct mutations and an "inverted" ddPCR assay design, based on employing probes matching wild-type alleles, capable of detecting the presence of multiple single nucleotide polymorphisms. The assays successfully detected and quantified somatic mutations commonly affecting enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) (Y641) and signal transducer and activator of transcription 6 (STAT6) (D419) hotspots in fresh tumor, FFPE, and liquid biopsies. The "inverted" ddPCR approach effectively reported any single nucleotide variant affecting either of these 2 hotspots as well. Finally, we could effectively multiplex hydrolysis probes targeting 2 additional lymphoma-related hotspots: myeloid differentiation primary response 88 (MYD88; L265P) and cyclin D3 (CCND3; I290R). Our suite of ddPCR assays provides sufficient analytical sensitivity and specificity for either the invasive or noninvasive detection of multiple recurrent somatic mutations in B-cell NHLs. © 2016 American Association for Clinical Chemistry.

A parallel-pipelined architecture for a multi carrier demodulator

NASA Astrophysics Data System (ADS)

Kwatra, S. C.; Jamali, M. M.; Eugene, Linus P.

1991-03-01

Analog devices have been used for processing the information on board the satellites. Presently, digital devices are being used because they are economical and flexible as compared to their analog counterparts. Several schemes of digital transmission can be used depending on the data rate requirement of the user. An economical scheme of transmission for small earth stations uses single channel per carrier/frequency division multiple access (SCPC/FDMA) on the uplink and time division multiplexing (TDM) on the downlink. This is a typical communication service offered to low data rate users in commercial mass market. These channels usually pertain to either voice or data transmission. An efficient digital demodulator architecture is provided for a large number of law data rate users. A demodulator primarily consists of carrier, clock, and data recovery modules. This design uses principles of parallel processing, pipelining, and time sharing schemes to process large numbers of voice or data channels. It maintains the optimum throughput which is derived from the designed architecture and from the use of high speed components. The design is optimized for reduced power and area requirements. This is essential for satellite applications. The design is also flexible in processing a group of a varying number of channels. The algorithms that are used are verified by the use of a computer aided software engineering (CASE) tool called the Block Oriented System Simulator. The data flow, control circuitry, and interface of the hardware design is simulated in C language. Also, a multiprocessor approach is provided to map, model, and simulate the demodulation algorithms mainly from a speed view point. A hypercude based architecture implementation is provided for such a scheme of operation. The hypercube structure and the demodulation models on hypercubes are simulated in Ada.

A parallel-pipelined architecture for a multi carrier demodulator. M.S. Thesis Final Technical Report, Jan. 1989 - Aug. 1990

NASA Technical Reports Server (NTRS)

Kwatra, S. C.; Jamali, M. M.; Eugene, Linus P.

1991-01-01

Analog devices have been used for processing the information on board the satellites. Presently, digital devices are being used because they are economical and flexible as compared to their analog counterparts. Several schemes of digital transmission can be used depending on the data rate requirement of the user. An economical scheme of transmission for small earth stations uses single channel per carrier/frequency division multiple access (SCPC/FDMA) on the uplink and time division multiplexing (TDM) on the downlink. This is a typical communication service offered to low data rate users in commercial mass market. These channels usually pertain to either voice or data transmission. An efficient digital demodulator architecture is provided for a large number of law data rate users. A demodulator primarily consists of carrier, clock, and data recovery modules. This design uses principles of parallel processing, pipelining, and time sharing schemes to process large numbers of voice or data channels. It maintains the optimum throughput which is derived from the designed architecture and from the use of high speed components. The design is optimized for reduced power and area requirements. This is essential for satellite applications. The design is also flexible in processing a group of a varying number of channels. The algorithms that are used are verified by the use of a computer aided software engineering (CASE) tool called the Block Oriented System Simulator. The data flow, control circuitry, and interface of the hardware design is simulated in C language. Also, a multiprocessor approach is provided to map, model, and simulate the demodulation algorithms mainly from a speed view point. A hypercude based architecture implementation is provided for such a scheme of operation. The hypercube structure and the demodulation models on hypercubes are simulated in Ada.

The GOES-R Rebroadcast (GRB) Data Stream Simulator

NASA Astrophysics Data System (ADS)

Dittberner, G. J.; Gibbons, K.; Czopkiewicz, E.; Miller, C.; Brown-Bergtold, B.; Haman, B.; Marley, S.

2013-12-01

GOES Rebroadcast (GRB) signals in the GOES-R era will replace the current legacy GOES Variable (GVAR) signal and will have substantially different characteristics, including a change in data rate from a single 2.1 Mbps stream to two digital streams of 15.5 Mbps each. Five GRB Simulators were developed as portable systems that output a high-fidelity stream of Consultative Committee for Space Data Systems (CCSDS) formatted GRB packet data equivalent to live GRB data. The data are used for on-site testing of user ingest and data handling systems known as field terminal sites. The GRB Simulator is a fully self-contained system which includes all software and hardware units needed for operation. The operator manages configurations to edit preferences, define individual test scenarios, and manage event logs and reports. Simulations are controlled by test scenarios, which are scripts that specify the test data and provide a series of actions for the GRB Simulator to perform when generating GRB output. Scenarios allow for the insertion of errors or modification of GRB packet headers for testing purposes. The GRB Simulator provides a built-in editor for managing scenarios. The GRB Simulator provides GRB data as either baseband (digital) or Intermediate Frequency (IF) output to the test system. GRB packet data are sent in the same two output streams used in the operational system: one for Left Hand Circular Polarization (LHCP) and one for Right Hand Circular Polarization (RHCP). Use of circular polarization in the operational system allows the transmitting antenna to multiplex the two digital streams into the same signal, thereby doubling the available bandwidth. The GRB Simulator is designed to be used at sites that receive the GRB downlink.

Radiographic applications of spatial frequency multiplexing

NASA Technical Reports Server (NTRS)

Macovski, A.

1981-01-01

The application of spacial frequency encoding techniques which allow different regions of the X-ray spectrum to be encoded on conventional radiographs was studied. Clinical considerations were reviewed, as were experimental studies involving the encoding and decoding of X-ray images at different energies and the subsequent processing of the data to produce images of specific materials in the body.

Automated hybridization/imaging device for fluorescent multiplex DNA sequencing

DOEpatents

Weiss, R.B.; Kimball, A.W.; Gesteland, R.F.; Ferguson, F.M.; Dunn, D.M.; Di Sera, L.J.; Cherry, J.L.

1995-11-28

A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, the enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber device with a beam of light from the imaging apparatus, excites fluorescence of the fluorescent product to produce a pattern of hybridization. The pattern of hybridization is imaged by a CCD camera component of the imaging apparatus to obtain a series of digital signals. These signals are converted by the controller apparatus into a string of nucleotides corresponding to the nucleotide sequence an automated sequence reader. The method and apparatus are also applicable to other membrane-based applications such as colony and plaque hybridization and Southern, Northern, and Western blots. 9 figs.

Automated hybridization/imaging device for fluorescent multiplex DNA sequencing

DOEpatents

Weiss, Robert B.; Kimball, Alvin W.; Gesteland, Raymond F.; Ferguson, F. Mark; Dunn, Diane M.; Di Sera, Leonard J.; Cherry, Joshua L.

1995-01-01

A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber device with a beam of light from the imaging apparatus, excites fluorescence of the fluorescent product to produce a pattern of hybridization. The pattern of hybridization is imaged by a CCD camera component of the imaging apparatus to obtain a series of digital signals. These signals are converted by the controller apparatus into a string of nucleotides corresponding to the nucleotide sequence an automated sequence reader. The method and apparatus are also applicable to other membrane-based applications such as colony and plaque hybridization and Southern, Northern, and Western blots.

Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping.

PubMed

Wood-Bouwens, Christina M; Ji, Hanlee P

2018-01-01

Droplet digital PCR (ddPCR) allows for accurate quantification of genetic events such as copy number variation and single nucleotide variants. Probe-based assays represent the current "gold-standard" for detection and quantification of these genetic events. Here, we introduce a cost-effective single color ddPCR assay that allows for single genome resolution quantification of copy number and single nucleotide variation.

High-Speed Optical Wide-Area Data-Communication Network

NASA Technical Reports Server (NTRS)

Monacos, Steve P.

1994-01-01

Proposed fiber-optic wide-area network (WAN) for digital communication balances input and output flows of data with its internal capacity by routing traffic via dynamically interconnected routing planes. Data transmitted optically through network by wavelength-division multiplexing in synchronous or asynchronous packets. WAN implemented with currently available technology. Network is multiple-ring cyclic shuffle exchange network ensuring traffic reaches its destination with minimum number of hops.

Digital-Difference Processing For Collision Avoidance.

NASA Technical Reports Server (NTRS)

Shores, Paul; Lichtenberg, Chris; Kobayashi, Herbert S.; Cunningham, Allen R.

1988-01-01

Digital system for automotive crash avoidance measures and displays difference in frequency between two sinusoidal input signals of slightly different frequencies. Designed for use with Doppler radars. Characterized as digital mixer coupled to frequency counter measuring difference frequency in mixer output. Technique determines target path mathematically. Used for tracking cars, missiles, bullets, baseballs, and other fast-moving objects.

Digital carrier demodulator employing components working beyond normal limits

NASA Technical Reports Server (NTRS)

Hurd, William J. (Inventor); Sadr, Ramin (Inventor)

1990-01-01

In a digital device, having an input comprised of a digital sample stream at a frequency F, a method is disclosed for employing a component designed to work at a frequency less than F. The method, in general, is comprised of the following steps: dividing the digital sample stream into odd and even digital samples streams each at a frequency of F/2; passing one of the digital sample streams through the component designed to work at a frequency less than F where the component responds only to the odd or even digital samples in one of the digital sample streams; delaying the other digital sample streams for the time it takes the digital sample stream to pass through the component; and adding the one digital sample stream after passing through the component with the other delayed digital sample streams. In the specific example, the component is a finite impulse response filter of the order ((N + 1)/2) and the delaying step comprised passing the other digital sample streams through a shift register for a time (in sampling periods) of ((N + 1)/2) + r, where r is a pipline delay through the finite impulse response filter.

Study of the true performance limits of the Astrometric Multiplexing Area Scanner (AMAS)

NASA Technical Reports Server (NTRS)

Frederick, L. W.; Mcalister, H. A.

1975-01-01

The Astrometric Multiplexing Area Scanner (AMAS) is an instrument designed to perform photoelectric long focus astrometry of small fields. Modulation of a telescope focal plane with a rotating Ronchi ruling produces a frequency modulated signal from which relative positions and magnitudes can be extracted. Evaluation instrumental precision, accuracy and resolution characteristics with respect to a variety of instrumental and cosmical parameters indicates 1.5 micron precision and accuracy for single stars under specific conditions. This value decreases for increased number of field stars, particularly for fainter stars.

Design and Performance of the Multiplexed SQUID/TES Array at Ninety Gigahertz

NASA Astrophysics Data System (ADS)

Stanchfield, Sara; Ade, Peter; Aguirre, James; Brevik, Justus A.; Cho, Hsiao-Mei; Datta, Rahul; Devlin, Mark; Dicker, Simon R.; Dober, Bradley; Duff, Shannon M.; Egan, Dennis; Ford, Pam; Hilton, Gene; Hubmayr, Johannes; Irwin, Kent; Knowles, Kenda; Marganian, Paul; Mason, Brian Scott; Mates, John A. B.; McMahon, Jeff; Mello, Melinda; Mroczkowski, Tony; Romero, Charles; Sievers, Jonathon; Tucker, Carole; Vale, Leila R.; Vissers, Michael; White, Steven; Whitehead, Mark; Ullom, Joel; Young, Alexander

2018-01-01

We present the array performance and astronomical images from early science results from MUSTANG-2, a 90 GHz feedhorn-coupled, microwave SQUID-multiplexed TES bolometer array operating on the Robert C. Byrd Green Bank Telescope (GBT). MUSTANG-2 was installed on the GBT on December 2, 2016 and immediately began commissioning efforts, followed by science observations, which are expected to conclude June 2017. The feedhorn and waveguide-probe-coupled detector technology is a mature technology, which has been used on instrument including the South Pole Telescope, the Atacama Cosmology Telescope, and the Atacama B-mode Search telescope. The microwave SQUID readout system developed for MUSTANG-2 currently reads out 66 detectors with a single coaxial cable and will eventually allow thousands of detectors to be multiplexed. This microwave SQUID multiplexer combines the proven abilities of millimeterwave TES detectors with the multiplexing capabilities of KIDs with no degradation in noise performance of the detectors. Each multiplexing device is read out using warm electronics consisting of a commercially available ROACH board, a DAC/ADC card, and an Intermediate Frequency mixer circuit. The hardware was originally developed by the UC Berkeley Collaboration for Astronomy Signal Processing and Electronic Research (CASPER) group, whose primary goal is to develop scalable FPGA-based hardware with the flexibility to be used in a wide range of radio signal processing applications. MUSTANG-2 is the first on-sky instrument to use microwave SQUID multiplexing and is available as a shared-risk/PI instrument on the GBT. In MUSTANG-2's first season 7 separate proposals were awarded a total of 230 hours of telescope time.

White noise of Nb-based microwave superconducting quantum interference device multiplexers with NbN coplanar resonators for readout of transition edge sensors

NASA Astrophysics Data System (ADS)

Kohjiro, Satoshi; Hirayama, Fuminori; Yamamori, Hirotake; Nagasawa, Shuichi; Fukuda, Daiji; Hidaka, Mutsuo

2014-06-01

White noise of dissipationless microwave radio frequency superconducting quantum interference device (RF-SQUID) multiplexers has been experimentally studied to evaluate their readout performance for transition edge sensor (TES) photon counters ranging from near infrared to gamma ray. The characterization has been carried out at 4 K, first to avoid the low-frequency fluctuations present at around 0.1 K, and second, for a feasibility study of readout operation at 4 K for extended applications. To increase the resonant Q at 4 K and maintain low noise SQUID operation, multiplexer chips consisting of niobium nitride (NbN)-based coplanar-waveguide resonators and niobium (Nb)-based RF-SQUIDs have been developed. This hybrid multiplexer exhibited 1 × 104 ≤ Q ≤ 2 × 104 and the square root of spectral density of current noise referred to the SQUID input √SI = 31 pA/√Hz. The former and the latter are factor-of-five and seven improvements from our previous results on Nb-based resonators, respectively. Two-directional readout on the complex plane of the transmission component of scattering matrix S21 enables us to distinguish the flux noise from noise originating from other sources, such as the cryogenic high electron mobility transistor (HEMT) amplifier. Systematic noise measurements with various microwave readout powers PMR make it possible to distinguish the contribution of noise sources within the system as follows: (1) The achieved √SI is dominated by the Nyquist noise from a resistor at 4 K in parallel to the SQUID input coil which is present to prevent microwave leakage to the TES. (2) The next dominant source is either the HEMT-amplifier noise (for small values of PMR) or the quantization noise due to the resolution of 300-K electronics (for large values of PMR). By a decrease of these noise levels to a degree that is achievable by current technology, we predict that the microwave RF-SQUID multiplexer can exhibit √SI ≤ 5 pA/√Hz, i.e., close to √SI of state-of-the-art DC-SQUID-based multiplexers.

Multiplex KRASG12/G13 mutation testing of unamplified cell-free DNA from the plasma of patients with advanced cancers using droplet digital polymerase chain reaction.

PubMed

Janku, F; Huang, H J; Fujii, T; Shelton, D N; Madwani, K; Fu, S; Tsimberidou, A M; Piha-Paul, S A; Wheler, J J; Zinner, R G; Naing, A; Hong, D S; Karp, D D; Cabrilo, G; Kopetz, E S; Subbiah, V; Luthra, R; Kee, B K; Eng, C; Morris, V K; Karlin-Neumann, G A; Meric-Bernstam, F

2017-03-01

Cell-free DNA (cfDNA) from plasma offers easily obtainable material for KRAS mutation analysis. Novel, multiplex, and accurate diagnostic systems using small amounts of DNA are needed to further the use of plasma cfDNA testing in personalized therapy. Samples of 16 ng of unamplified plasma cfDNA from 121 patients with diverse progressing advanced cancers were tested with a KRASG12/G13 multiplex assay to detect the seven most common mutations in the hotspot of exon 2 using droplet digital polymerase chain reaction (ddPCR). The results were retrospectively compared to mutation analysis of archival primary or metastatic tumor tissue obtained at different points of clinical care. Eighty-eight patients (73%) had KRASG12/G13 mutations in archival tumor specimens collected on average 18.5 months before plasma analysis, and 78 patients (64%) had KRASG12/G13 mutations in plasma cfDNA samples. The two methods had initial overall agreement in 103 (85%) patients (kappa, 0.66; ddPCR sensitivity, 84%; ddPCR specificity, 88%). Of the 18 discordant cases, 12 (67%) were resolved by increasing the amount of cfDNA, using mutation-specific probes, or re-testing the tumor tissue, yielding overall agreement in 115 patients (95%; kappa 0.87; ddPCR sensitivity, 96%; ddPCR specificity, 94%). The presence of ≥ 6.2% of KRASG12/G13 cfDNA in the wild-type background was associated with shorter survival (P = 0.001). Multiplex detection of KRASG12/G13 mutations in a small amount of unamplified plasma cfDNA using ddPCR has good sensitivity and specificity and good concordance with conventional clinical mutation testing of archival specimens. A higher percentage of mutant KRASG12/G13 in cfDNA corresponded with shorter survival. © The Author 2016. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

All-optical wavelength conversion for mode division multiplexed superchannels.

PubMed

Gong, Jiaxin; Xu, Jing; Luo, Ming; Li, Xiang; Qiu, Ying; Yang, Qi; Zhang, Xinliang; Yu, Shaohua

2016-04-18

We report in this work the first all-optical wavelength conversion (AOWC) of a mode division multiplexed (MDM) superchannel consisting of 2N modes by dividing the superchannel into N single-mode (SM) tributaries, wavelength converting N SM signals using well developed SM-AOWC techniques, and finally combining the N SM tributaries back to an MDM superchannel at the converted wavelength, inspired by the idea of using SM filtering techniques to filter multimode signals in astronomy. The conversions between multimode and SM are realized by 3D laser-writing photonic lanterns and SM-AOWCs are realized based on polarization insensitive four wave mixing (FWM) configuration in N semiconductor optical amplifiers (SOAs). As a proof of concept demonstration, the conversion of a 6-mode MDM superchannel with each mode modulated with orthogonal frequency division multiplexed (OFDM) quadrature phase-shift keying (QPSK)/16 quadrature amplitude modulation (QAM) signals is demonstrated in this work, indicating that the scheme is transparent to data format, polarization and compatible with multi-carrier signals. Data integrity of the converted superchannel has been verified by using coherent detection and digital signal processing (DSP). Bit error rates (BERs) below the forward error correction (FEC) hard limit (3.8 × 10^-3) have been obtained for QPSK modulation at a net bitrate of 104.2 Gbit/s and BERs below the soft decision FEC threshold (1.98 × 10^-2) have been achieved for 16-QAM format, giving a total aggregate bit rate of 185.8 Gbit/s when taking 20% coding overhead into account. Add and drop functionalities that usually come along with wavelength conversion in flexible network nodes have also been demonstrated. The working conditions of the SOAs, especially the pump and signal power levels, are critical for the quality of the converted signal and have been thoroughly discussed. The impact of imbalanced FWM conversion efficiency among different SM tributaries has also been analyzed. This work illustrates a promising way to perform all-optical signal processing for MDM superchannels.

The 30/20 Gigahertz transponder study. [wideband multichannel transponders for a communications satellite

NASA Technical Reports Server (NTRS)

1980-01-01

Design features and performance parameters are described for three types of wideband multiple channel satellite transponders for use in a 30/20 GHz communications satellite, which provides high data rate trunking service to ten ground station terminals. The three types of transponder are frequency division multiplex (FDM), time division multiplex (TDM), and a hybrid transponder using a combination of FDM and TDM techniques. The wideband multiple beam trunking concept, the traffic distribution between the trunking terminals, and system design constraints are discussed. The receiver front end design, the frequency conversion scheme, and the local oscillator design are described including the thermal interface between the transponders and the satellite. The three designs are compared with regard to performance, weight, power, cost and initial technology. Simplified block diagrams of the baseline transponder designs are included.

Optical demodulation system for digitally encoded suspension array in fluoroimmunoassay

NASA Astrophysics Data System (ADS)

He, Qinghua; Li, Dongmei; He, Yonghong; Guan, Tian; Zhang, Yilong; Shen, Zhiyuan; Chen, Xuejing; Liu, Siyu; Lu, Bangrong; Ji, Yanhong

2017-09-01

A laser-induced breakdown spectroscopy and fluorescence spectroscopy-coupled optical system is reported to demodulate digitally encoded suspension array in fluoroimmunoassay. It takes advantage of the plasma emissions of assembled elemental materials to digitally decode the suspension array, providing a more stable and accurate recognition to target biomolecules. By separating the decoding procedure of suspension array and adsorption quantity calculation of biomolecules into two independent channels, the cross talk between decoding and label signals in traditional methods had been successfully avoided, which promoted the accuracy of both processes and realized more sensitive quantitative detection of target biomolecules. We carried a multiplexed detection of several types of anti-IgG to verify the quantitative analysis performance of the system. A limit of detection of 1.48×10-10 M was achieved, demonstrating the detection sensitivity of the optical demodulation system.

The E and B EXperiment: EBEX

NASA Astrophysics Data System (ADS)

Helson, Kyle R.

2015-08-01

We report on the status of the E and B Experiment (EBEX) a balloon-borne polarimeter designed to measure the polarization of the cosmic microwave background radiation. The instrument employs a 1.5 meter Gregorian Mizuguchi-Dragone telescope providing 8 arc-minute resolution at three bands centered on 150, 250, and 410 GHz. A continuously rotating achromatic half wave plate, mounted on a superconducting magnetic bearing, and a polarizing grid give EBEX polarimetric capabilities. Radiation is detected with a kilo-pixel array of transition edge sensor (TES) bolometers that are cooled to 0.25 K. The detectors are readout using SQUID current amplifiers and a digital frequency-domain multiplexing system in which 16 detectors are readout simultaneously with two wires. EBEX is the first instrument to implement TESs and such readout system on board a balloon-borne platform. EBEX was launched from the Antarctic in December 2012 on an 11-day long-duration balloon flight. This presentation will provide an overview of the instrument and discuss the flight and status of the data analysis. We also discuss the next generation of EBEX called EBEX10k, currently in development.

DSP-based CSO cancellation technique for RoF transmission system implemented by using directly modulated laser.

PubMed

Kim, Byung Gon; Bae, Sung Hyun; Kim, Hoon; Chung, Yun C

2017-05-29

We propose and demonstrate a simple composite second-order (CSO) cancellation technique based on the digital signal processing (DSP) for the radio-over-fiber (RoF) transmission system implemented by using directly modulated lasers (DMLs). When the RoF transmission system is implemented by using DMLs, its performance could be limited by the CSO distortions caused by the interplay between the DML's chirp and fiber's chromatic dispersion. We present the theoretical analysis of these nonlinear distortions and show that they can be suppressed at the receiver by using a simple DSP. To verify the effectiveness of the proposed technique, we demonstrate the transmission of twenty-four 100-MHz filtered orthogonal frequency-division multiplexing (f-OFDM) signals in 64 quadrature amplitude modulation (QAM) format over 20 km of the standard single-mode fiber (SSMF). The results show that, by using the proposed technique, we can suppress the CSO distortion components by >10 dB and achieve the error-vector magnitude performance better than 6% even after the 20-km long SSMF transmission.

Multiplexed Elimination of Wild-Type DNA and High-Resolution Melting Prior to Targeted Resequencing of Liquid Biopsies.

PubMed

Ladas, Ioannis; Fitarelli-Kiehl, Mariana; Song, Chen; Adalsteinsson, Viktor A; Parsons, Heather A; Lin, Nancy U; Wagle, Nikhil; Makrigiorgos, G Mike

2017-10-01

The use of clinical samples and circulating cell-free DNA (cfDNA) collected from liquid biopsies for diagnostic and prognostic applications in cancer is burgeoning, and improved methods that reduce the influence of excess wild-type (WT) portion of the sample are desirable. Here we present enrichment of mutation-containing sequences using enzymatic degradation of WT DNA. Mutation enrichment is combined with high-resolution melting (HRM) performed in multiplexed closed-tube reactions as a rapid, cost-effective screening tool before targeted resequencing. We developed a homogeneous, closed-tube approach to use a double-stranded DNA-specific nuclease for degradation of WT DNA at multiple targets simultaneously. The No Denaturation Nuclease-assisted Minor Allele Enrichment with Probe Overlap (ND-NaME-PrO) uses WT oligonucleotides overlapping both strands on putative DNA targets. Under conditions of partial denaturation (DNA breathing), the oligonucleotide probes enhance double-stranded DNA-specific nuclease digestion at the selected targets, with high preference toward WT over mutant DNA. To validate ND-NaME-PrO, we used multiplexed HRM, digital PCR, and MiSeq targeted resequencing of mutated genomic DNA and cfDNA. Serial dilution of KRAS mutation-containing DNA shows mutation enrichment by 10- to 120-fold and detection of allelic fractions down to 0.01%. Multiplexed ND-NaME-PrO combined with multiplexed PCR-HRM showed mutation scanning of 10-20 DNA amplicons simultaneously. ND-NaME-PrO applied on cfDNA from clinical samples enables mutation enrichment and HRM scanning over 10 DNA targets. cfDNA mutations were enriched up to approximately 100-fold (average approximately 25-fold) and identified via targeted resequencing. Closed-tube homogeneous ND-NaME-PrO combined with multiplexed HRM is a convenient approach to efficiently enrich for mutations on multiple DNA targets and to enable prescreening before targeted resequencing. © 2017 American Association for Clinical Chemistry.

47 CFR 80.359 - Frequencies for digital selective calling (DSC).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Frequencies for digital selective calling (DSC... for digital selective calling (DSC). (a) General purpose calling. The following table describes the calling frequencies for use by authorized ship and coast stations for general purpose DSC. There are three...

Notes on a broad-band variant of the NCER seismic data multiplex system for use with field tape recorders

USGS Publications Warehouse

Eaton, Jerry P.

1976-01-01

Tests of the standard NCER multiplex system recorded and played back on both the Bell and Howell 3700B (about 0.1% tape speed variation) and on the Sony TC-126 cassette recorder (about 1% tape speed variation) showed that subtractive compensation employing a reference frequency multiplexed on the data track was remarkably effective in reducing tape-speed-variation-induced noise 1 and, hence, in increasing the dynamic range of the record/playback system. Further tests suggested that the 0 to 30 Hz bandwidth of the standard system (8 data channels) might be increased substantially, at the 'price' of reducing the number of data channels to 3 or 4, without serious loss of dynamic range if subtractive compensation could be implemented effectively with the broader-band system.

A MGy radiation-hardened sensor instrumentation link for nuclear reactor monitoring and remote handling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Verbeeck, Jens; Cao, Ying; Van Uffelen, Marco

Decommissioning, dismantling and remote handling applications in nuclear facilities all require robotic solutions that are able to survive in radiation environments. Recently raised safety, radiation hardness and cost efficiency demands from both the nuclear regulatory and the society impose severe challenges in traditional methods. For example, in case of the dismantling of the Fukushima sites, solutions that survive accumulated doses higher than 1 MGy are mandatory. To allow remote operation of these tools in nuclear environments, electronics were used to be shielded with several centimeters of lead or even completely banned in these solutions. However, shielding electronics always leads tomore » bulky and heavy solutions, which reduces the flexibility of robotic tools. It also requires longer repair time and produces extra waste further in a dismantling or decommissioning cycle. In addition, often in current reactor designs, due to size restrictions and the need to inspect very tight areas there are limitations to the use of shielding. A MGy radiation-hardened sensor instrumentation link developed by MAGyICS provides a solution to build a flexible, easy removable and small I and C module with MGy radiation tolerance without any shielding. Hereby it removes all these pains to implement electronics in robotic tools. The demonstrated solution in this poster is developed for ITER Remote Handling equipments operating in high radiation environments (>1 MGy) in and around the Tokamak. In order to obtain adequately accurate instrumentation and control information, as well as to ease the umbilical management, there is a need of front-end electronics that will have to be located close to those actuators and sensors on the remote handling tool. In particular, for diverter remote handling, it is estimated that these components will face gamma radiation up to 300 Gy/h (in-vessel) and a total dose of 1 MGy. The radiation-hardened sensor instrumentation link presented here, consists of multiple in-house developed, state-of-the-art building blocks. They have all been assessed and characterized up to a dose higher than 1 MGy. The first block in the signal chain is a smaller than 1 uV offset, low noise instrumentation amplifier that has a programmable gain between 8 and 256. This amplifier is followed by a high precision 16 bit ADC. Its main function is to digitize the amplified signal coming from the amplifier. The output of the ADC is a serial digital data stream. The next block is a 8 channel digital multiplexer. It converts the 8 digital data streams into 1 digital data stream. Hereby the instrumentation solution reduces the number of cables from 16 to 1 (8* 2 analogue differential signals). In addition, the multiplexer modules can be combined with other modules to reduce even further the number of cables. Every instrumentation solution requires a stable high precision voltage reference. Therefore also a bandgap reference has been developed and assessed under gamma irradiation. A low jitter, 10 MHz clock generator has been developed and qualified to clock the ADC and the multiplexer with high accuracy. Finally, an on-chip radiation-hard temperature sensor is also included. A complete remote, real-time test setup was prepared by MAGyICS in cooperation with Fusion for Energy to qualify the sensor instrumentation link at SCK-CEN. It is qualified by closely following the ESCC22900 space standard for electronics used in a radiation environment. The main benefit of the sensor instrumentation solution discussed here is that it can be directly employed in a MGy-level accumulated dose radiation environment, therefore it can digitize and multiplex sensor readout values early in the signal chain. Hereby the sensor values are not distorted by external interferences on the long transmission cable. Moreover it allows readout and digitize multiple low-bandwidth sensors ( pressure and temperature sensors, thermocouples, angular resolvers and LVDTs). Hence these instrumentation modules can be used to follow the trend of the digitalization in the nuclear industry. (authors)« less

Demonstration of a scalable frequency-domain readout of metallic magnetic calorimeters by means of a microwave SQUID multiplexer

NASA Astrophysics Data System (ADS)

Kempf, Sebastian; Wegner, Mathias; Fleischmann, Andreas; Gastaldo, Loredana; Herrmann, Felix; Papst, Maximilian; Richter, Daniel; Enss, Christian

2017-01-01

We report on the first demonstration of a scalable GHz frequency-domain readout of metallic magnetic calorimeters (MMCs) using a 64 pixel detector array that is read out by an integrated, on-chip microwave SQUID multiplexer. The detector array is optimized for detecting soft X-ray photons and the multiplexer is designed to provide a signal rise time τrise<400 ns and an intrinsic energy sensitivity ɛ <30 h . This results in an expected energy resolution Δ EFWHM <10 eV . We measured a signal rise time τrise as low as 90 ns and an energy resolution Δ EFWHM as low as 50 eV for 5.9 keV photons. The rise time is about an order of magnitude faster compared to other multiplexed low-temperature microcalorimeters and close to the intrinsic value set by the coupling between electron and spins. The energy resolution is degraded with respect to our design value due to a rather low intrinsic quality factor of the microwave resonators that is caused by the quality of the Josephson junction of the associated rf-SQUID as well as an elevated chip temperature as compared to the heat bath. Though the achieved energy resolution is not yet compatible with state-of-the-art single-channel MMCs, this demonstration of a scalable readout approach for MMCs in combination with the full understanding of the device performance showing ways how to improve represents an important milestone for the development of future large-scale MMC detector arrays.

Multiplex PCR reveals that viruses are more frequent than bacteria in children with cystic fibrosis.

PubMed

Miró-Cañís, Sílvia; Capilla-Rubio, Sílvia; Marzo-Checa, Laura; Fontanals-Aymerich, Dionisia; Sanfeliu-Sala, Isabel; Espasa-Soley, Mateu; Asensio-de-la-Cruz, Oscar

2017-01-01

Cystic fibrosis is a degenerative disease characterized by progressive epithelial secretory gland dysfunction associated with repeated respiratory infections. Bacterial infections are very frequent in children with cystic fibrosis, but because rapid METHODS: for screening for the wide variety of potentially involved viruses were unavailable until recently, the frequency of viral presence is unknown. Multiplex PCR enables screening for many viruses involved in respiratory infections. This study aimed to evaluate the frequency of viruses and bacteria in respiratory specimens from children with cystic fibrosis and to clarify the incidence and characteristics (seasonality and age of patients) of different viruses detected in children with cystic fibrosis. In this 2-year prospective study, we obtained paired nasopharyngeal-swab and sputum specimens from children with cystic fibrosis during clinical respiratory examinations separated by at least 14days. We analyzed viruses in nasopharyngeal-swab specimens with multiplex PCR and bacteria in sputum with standard methods. We analyzed 368 paired specimens from 33 children. We detected viruses in 154 (41.8%) and bacteria in 132 (35.9%). Bacteria were commoner in spring and summer; viruses were commoner in autumn and winter. In every season, Staphylococcus aureus was the commonest bacteria and rhinovirus was the commonest virus. Nearly all infections with Haemophilus influenzae occurred in autumn and winter. Viruses were more prevalent in children <5 years old, and bacteria were more prevalent in children ≥12 years old. Multiplex PCR screening for respiratory viruses is feasible in children with cystic fibrosis; the clinical implications of screening warrant further study. Copyright © 2016 Elsevier B.V. All rights reserved.

Combining spatial domain multiplexing and orbital angular momentum of photon-based multiplexing to increase the bandwidth of optical fiber communication systems

NASA Astrophysics Data System (ADS)

Murshid, Syed; Alanzi, Saud; Hridoy, Arnob; Lovell, Gregory L.; Parhar, Gurinder; Chakravarty, Abhijit; Chowdhury, Bilas

2016-06-01

Spatial domain multiplexing/space division multiplexing (SDM) can increase the bandwidth of existing and futuristic optical fibers by an order of magnitude or more. In the SDM technique, we launch multiple single-mode pigtail laser sources of the same wavelength into a carrier multimode fiber at different angles. The launching angles decide the output of the carrier fiber by allocating separate spatial locations for each channel. Each channel follows a helical trajectory while traversing the length of the carrier fiber, thereby allowing spatial reuse of optical frequencies. We launch light from five different single-mode pigtail laser sources (of same wavelength) at different angles (with respect to the axis of the carrier fiber) into the carrier fiber. Owing to helical propagation, five distinct concentric donut-shaped rings with negligible crosstalk at the output end of the fiber were obtained. These SDM channels also exhibit orbital angular momentum (OAM), thereby adding an extradegree of photon freedom. We present the experimental data of five spatially multiplexed channels and compare them with simulated results to show that this technique can potentially improve the data capacity of optical fibers by an order of magnitude: A factor of five using SDM and another factor of two using OAM.

An order of magnitude improvement in optical fiber bandwidth using spatial domain multiplexing/space division multiplexing (SDM) in conjunction with orbital angular momentum (OAM)

NASA Astrophysics Data System (ADS)

Murshid, Syed; Alanzi, Saud; Hridoy, Arnob; Lovell, Greg; Parhar, Gurinder; Chakravarty, Abhijit; Chowdhury, Bilas

2014-09-01

Spatial Domain Multiplexing/Space Division Multiplexing (SDM) can increase the bandwidth of existing and futuristic optical fibers by an order of magnitude or more. In the SDM technique, we launch multiple single mode pigtail laser sources of same wavelength into a carrier fiber at different angles. The launching angles decide the output of the carrier fiber by allocating separate spatial locations for each channel. Each channel follows a helical trajectory while traversing the length of the carrier fiber, thereby allowing spatial reuse of optical frequencies. In this endeavor we launch light from five different single mode pigtail laser sources at different angles (with respect to the axis of the carrier fiber) into the carrier fiber. Owing to helical propagation we get five distinct concentric donut shaped rings with negligible crosstalk at the output end of the fiber. These SDM channels also exhibit Orbital Angular Momentum (OAM), thereby adding an extra degree of photon freedom. We present the experimental data of five spatially multiplexed channels and compare them with simulated results to show that this technique can potentially improve the data capacity of optical fibers by an order of magnitude: A factor of five using SDM and another factor of two using OAM.

Multiplexed Cassegrain Reflector Antenna for Simultaneous Generation of Three Orbital Angular Momentum (OAM) Modes

PubMed Central

Byun, Woo Jin; Kim, Kwang Seon; Kim, Bong Su; Lee, Young Seung; Song, Myung Sun; Choi, Hyung Do; Cho, Yong Heui

2016-01-01

A multiplexed Cassegrain reflector antenna with a 2 × 2 open-ended rectangular waveguide (OERW) matrix feed and an orbital angular momentum (OAM) mode mux is proposed for the simultaneous generation of three OAM modes (l = 0, ±1). The OAM mode mux (OMM) was designed using sequential combinations of quadrature hybrids, crossovers, and phase shifters to multiplex and demultiplex three OAM modes at the same time. The 2 × 2 OERW matrix feed and the OMM were separately measured and their performances were verified according to proposed theories. A near-field antenna measurement for a multiplexed Cassegrain reflector antenna was conducted to obtain the far-field magnitude and phase patterns around polar elevation angle θ and azimuthal angle ϕ, thus confirming that our antenna can produce three OAM modes simultaneously. We also measured the communication link characteristics of two identical multiplexed antennas. The measurement results show that the channel isolation of three OAM modes is more than 12.7 [dB] and 17 [dB] for fixed and compensated receiver positions, respectively, indicating that the proposed antenna system can be used for independent communication links with the same frequency and polarisation. PMID:27252079

The Digital Data Acquisition System for the Russian VLBI Network of New Generation

NASA Technical Reports Server (NTRS)

Fedotov, Leonid; Nosov, Eugeny; Grenkov, Sergey; Marshalov, Dmitry

2010-01-01

The system consists of several identical channels of 1024 MHz bandwidth each. In each channel, the RF band is frequency-translated to the intermediate frequency range 1 - 2 GHz. Each channel consists of two parts: the digitizer and Mark 5C recorder. The digitizer is placed on the antenna close to the corresponding Low-Noise Amplifier output and consists of the analog frequency converter, ADC, and a device for digital processing of the signals using FPGA. In the digitizer the subdigitization on frequency of 2048 MHz is used. For producing narrow-band channels and to interface with existing data acquisition systems, the polyphase filtering with FPGA can be used. Digital signals are re-quantized to 2-bits in the FPGA and are transferred to an input of Mark 5C through a fiber line. The breadboard model of the digitizer is being tested, and the data acquisition system is being designed.

An all digital low data rate communication system

NASA Technical Reports Server (NTRS)

Chen, C.; Fan, M.

1973-01-01

The advent of digital hardwares has made it feasible to implement many communication system components digitally. With the exception of frequency down conversion, the proposed low data rate communication system uses digital hardwares completely. Although the system is designed primarily for deep space communications with large frequency uncertainty and low signal-to-noise ratio, it is also suitable for other low data rate applications with time-shared operation among a number of channels. Emphasis is placed on the fast Fourier transform receiver and the automatic frequency control via digital filtering. The speed available from the digital system allows sophisticated signal processing to reduce frequency uncertainty and to increase the signal-to-noise ratio. The practical limitations of the system such as the finite register length are examined. It is concluded that the proposed all-digital system is not only technically feasible but also has potential cost reduction over the existing receiving systems.

High-speed light field camera and frequency division multiplexing for fast multi-plane velocity measurements.

PubMed

Fischer, Andreas; Kupsch, Christian; Gürtler, Johannes; Czarske, Jürgen

2015-09-21

Non-intrusive fast 3d measurements of volumetric velocity fields are necessary for understanding complex flows. Using high-speed cameras and spectroscopic measurement principles, where the Doppler frequency of scattered light is evaluated within the illuminated plane, each pixel allows one measurement and, thus, planar measurements with high data rates are possible. While scanning is one standard technique to add the third dimension, the volumetric data is not acquired simultaneously. In order to overcome this drawback, a high-speed light field camera is proposed for obtaining volumetric data with each single frame. The high-speed light field camera approach is applied to a Doppler global velocimeter with sinusoidal laser frequency modulation. As a result, a frequency multiplexing technique is required in addition to the plenoptic refocusing for eliminating the crosstalk between the measurement planes. However, the plenoptic refocusing is still necessary in order to achieve a large refocusing range for a high numerical aperture that minimizes the measurement uncertainty. Finally, two spatially separated measurement planes with 25×25 pixels each are simultaneously acquired with a measurement rate of 0.5 kHz with a single high-speed camera.

A rotor-mounted digital instrumentation system for helicopter blade flight research measurements

NASA Technical Reports Server (NTRS)

Knight, V. H., Jr.; Haywood, W. S., Jr.; Williams, M. L.

1978-01-01

A rotor mounted flight instrumentation system developed for helicopter rotor blade research is described. The system utilizes high speed digital techniques to acquire research data from miniature pressure transducers on advanced rotor airfoils which are flight tested on an AH-1G helicopter. The system employs microelectronic pulse code modulation (PCM) multiplexer digitizer stations located remotely on the blade and in a hub mounted metal canister. As many as 25 sensors can be remotely digitized by a 2.5 mm thick electronics package mounted on the blade near the tip to reduce blade wiring. The electronics contained in the canister digitizes up to 16 sensors, formats these data with serial PCM data from the remote stations, and transmits the data from the canister which is above the plane of the rotor. Data are transmitted over an RF link to the ground for real time monitoring and to the helicopter fuselage for tape recording. The complete system is powered by batteries located in the canister and requires no slip rings on the rotor shaft.

Minimal Residual Disease Monitoring of Acute Myeloid Leukemia by Massively Multiplex Digital PCR in Patients with NPM1 Mutations.

PubMed

Mencia-Trinchant, Nuria; Hu, Yang; Alas, Maria Antonina; Ali, Fatima; Wouters, Bas J; Lee, Sangmin; Ritchie, Ellen K; Desai, Pinkal; Guzman, Monica L; Roboz, Gail J; Hassane, Duane C

2017-07-01

The presence of minimal residual disease (MRD) is widely recognized as a powerful predictor of therapeutic outcome in acute myeloid leukemia (AML), but methods of measurement and quantification of MRD in AML are not yet standardized in clinical practice. There is an urgent, unmet need for robust and sensitive assays that can be readily adopted as real-time tools for disease monitoring. NPM1 frameshift mutations are an established MRD marker present in half of patients with cytogenetically normal AML. However, detection is complicated by the existence of hundreds of potential frameshift insertions, clonal heterogeneity, and absence of sequence information when the NPM1 mutation is identified using capillary electrophoresis. Thus, some patients are ineligible for NPM1 MRD monitoring. Furthermore, a subset of patients with NPM1-mutated AML will have false-negative MRD results because of clonal evolution. To simplify and improve MRD testing for NPM1, we present a novel digital PCR technique composed of massively multiplex pools of insertion-specific primers that selectively detect mutated but not wild-type NPM1. By measuring reaction end points using digital PCR technology, the resulting single assay enables sensitive and specific quantification of most NPM1 exon 12 mutations in a manner that is robust to clonal heterogeneity, does not require NPM1 sequence information, and obviates the need for maintenance of hundreds of type-specific assays and associated plasmid standards. Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

Development of a multiplexed readout with high position resolution for positron emission tomography

NASA Astrophysics Data System (ADS)

Lee, Sangwon; Choi, Yong; Kang, Jihoon; Jung, Jin Ho

2017-04-01

Detector signals for positron emission tomography (PET) are commonly multiplexed to reduce the number of digital processing channels so that the system can remain cost effective while also maintaining imaging performance. In this work, a multiplexed readout combining Anger position estimation algorithm and position decoder circuit (PDC) was developed to reduce the number of readout channels by a factor of 24, 96-to-4. The data acquisition module consisted of a TDC (50 ps resolution), 4-channel ADCs (12 bit, 105 MHz sampling rate), 2 GB SDRAM and USB3.0. The performance of the multiplexed readout was assessed with a high-resolution PET detector block composed of 2×3 detector modules, each consisting of an 8×8 array of 1.52×1.52×6 mm3 LYSO, a 4×4 array of 3×3 mm2 silicon photomultiplier (SiPM) and 13.4×13.4 mm2 light guide with 0.7 mm thickness. The acquired flood histogram showed that all 384 crystals could be resolved. The average energy resolution at 511 keV was 13.7±1.6% full-width-at-half-maximum (FWHM) and the peak-to-valley ratios of the flood histogram on the horizontal and vertical lines were 18.8±0.8 and 22.8±1.3, respectively. The coincidence resolving time of a pair of detector blocks was 6.2 ns FWHM. The reconstructed phantom image showed that rods down to a diameter of 1.6 mm could be resolved. The results of this study indicate that the multiplexed readout would be useful in developing a PET with a spatial resolution less than the pixel size of the photosensor, such as a SiPM array.

Linkage analysis with multiplexed short tandem repeat polymorphisms using infrared fluorescence and M13 tailed primers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oetting, W.S.; Lee, H.K.; Flanders, D.J.

The use of short tandem repeat polymorphisms (STRPs) as marker loci for linkage analysis is becoming increasingly important due to their large numbers in the human genome and their high degree of polymorphism. Fluorescence-based detection of the STRP pattern with an automated DNA sequencer has improved the efficiency of this technique by eliminating the need for radioactivity and producing a digitized autoradiogram-like image that can be used for computer analysis. In an effort to simplify the procedure and to reduce the cost of fluorescence STRP analysis, we have developed a technique known as multiplexing STRPs with tailed primers (MSTP) usingmore » primers that have a 19-bp extension, identical to the sequence of an M13 sequencing primer, on the 5{prime} end of the forward primer in conjunction with multiplexing several primer pairs in a single polymerase chain reaction (PCR) amplification. The banding pattern is detected with the addition of the M13 primer-dye conjugate as the sole primer conjugated to the fluorescent dye, eliminating the need for direct conjugation of the infrared fluorescent dye to the STRP primers. The use of MSTP for linkage analysis greatly reduces the number of PCR reactions. Up to five primer pairs can be multiplexed together in the same reaction. At present, a set of 148 STRP markers spaced at an average genetic distance of 28 cM throughout the autosomal genome can be analyzed in 37 sets of multiplexed amplification reactions. We have automated the analysis of these patterns for linkage using software that both detects the STRP banding pattern and determines their sizes. This information can then be exported in a user-defined format from a database manager for linkage analysis. 15 refs., 2 figs., 4 tabs.« less

Digital frequency control of satellite frequency standards. [Defense Navigation Satellites

NASA Technical Reports Server (NTRS)

Nichols, S. A.

1973-01-01

In the Frequency and Time Standard Development Program of the TIMATION System, a new miniaturized rubidium vapor frequency standard has been tested and analyzed for possible use on the TIMATION 3A launch, as part of the Defense Navigation Satellite Development Program. The design and construction of a digital frequency control was required to remotely control this rubidium vapor frequency standard as well as the quartz oscillator in current use. This control must be capable of accepting commands from a satellite telemetry system, verify that the correct commands have been sent and control the frequency to the requirements of the system. Several modifications must be performed to the rubidium vapor frequency standard to allow it to be compatible with the digital frequency control. These include the addition of a varactor to voltage tune the coarse range of the flywheel oscillator, and a modification to supply the C field current externally. The digital frequency control for the rubidium vapor frequency standard has been successfully tested in prototype form.

Effect of Loss on Multiplexed Single-Photon Sources (Open Access Publisher’s Version)

DTIC Science & Technology

2015-04-28

lossy components on near- and long-term experimental goals, we simulate themultiplexed sources when used formany- photon state generation under various...efficient integer factorization and digital quantum simulation [7, 8], which relies critically on the development of a high-performance, on-demand photon ...SPDC) or spontaneous four-wave mixing: parametric processes which use a pump laser in a nonlinearmaterial to spontaneously generate photon pairs

Development Test II of Time Division Digital Multiplexer TD-1069( )/G

DTIC Science & Technology

1976-11-01

fungi: (1) Aspergillus flavus (2) Aspergillus niger (3) Aspergillus versicolor (4) Penicillium funicolosum (5) Chaetomium globosum c The fungi... Aspergillus flavus and a negligible amount of Aspergillus niger were observed on the exterior surface of the test item. 2-80 ■ ■■--’ — (2) Top...interior. The wire ties maintained a moderate amount of Aspergillus veraicolor and spotted colonies of Penicillium funiculosum. The voltage select

640 X 480 MOS PtSi IR sensor

NASA Astrophysics Data System (ADS)

Sauer, Donald J.; Shallcross, Frank V.; Hseuh, Fu-Lung; Meray, Grazyna M.; Levine, Peter A.; Gilmartin, Harvey R.; Villani, Thomas S.; Esposito, Benjamin J.; Tower, John R.

1991-12-01

The design of a 1st and 2nd generation 640(H) X 480(V) element PtSi Schottky-barrier infrared image sensor employing a low-noise MOS X-Y addressable readout multiplexer and on-chip low-noise output amplifier is described. Measured performance characteristics for Gen 1 devices are presented along with calculated performance for the Gen 2 design. A multiplexed horizontal/vertical input address port and on-chip decoding is used to load scan data into CMOS horizontal and vertical scanning registers. This allows random access to any sub-frame in the 640 X 480 element focal plane array. By changing the digital pattern applied to the vertical scan register, the FPA can be operated in either an interlaced or non- interlaced format, and the integration time may be varied over a wide range (60 microsecond(s) to > 30 ms, for RS170 operation) resulting in a form of 'electronic shutter,' or variable exposure control. The pixel size of 24-micrometers X 24-micrometers results in a fill factor of 38% for 1.5-micrometers process design rules. The overall die size for the IR imager is 13.7 mm X 17.2 mm. All digital inputs to the chip are TTL compatible and include ESD protection.

A Robust Protocol for Using Multiplexed Droplet Digital PCR to Quantify Somatic Copy Number Alterations in Clinical Tissue Specimens.

PubMed

Hughesman, Curtis B; Lu, X J David; Liu, Kelly Y P; Zhu, Yuqi; Poh, Catherine F; Haynes, Charles

2016-01-01

The ability of droplet digital PCR (ddPCR) to accurately determine the concentrations of amplifiable targets makes it a promising platform for measuring copy number alterations (CNAs) in genomic biomarkers. However, its application to clinical samples, particularly formalin-fixed paraffin-embedded specimens, will require strategies to reliably determine CNAs in DNA of limited quantity and quality. When applied to cancerous tissue, those methods must also account for global genetic instability and the associated probability that the abundance(s) of one or more chosen reference loci do not represent the average ploidy of cells comprising the specimen. Here we present an experimental design strategy and associated data analysis tool that enables accurate determination of CNAs in a panel of biomarkers using multiplexed ddPCR. The method includes strategies to optimize primer and probes design to cleanly segregate droplets in the data output from reaction wells amplifying multiple independent templates, and to correct for bias from artifacts such as DNA fragmentation. We demonstrate how a panel of reference loci can be used to determine a stable CNA-neutral benchmark. These innovations, when taken together, provide a comprehensive strategy that can be used to reliably detect biomarker CNAs in DNA extracted from either frozen or FFPE tissue biopsies.

107 Gb/s coherent optical OFDM transmission over 1000-km SSMF fiber using orthogonal band multiplexing.

PubMed

Shieh, W; Yang, Q; Ma, Y

2008-04-28

Coherent optical OFDM (CO-OFDM) has emerged as an attractive modulation format for the forthcoming 100 Gb/s Ethernet. However, even the spectral-efficient implementation of CO-OFDM requires digital-to-analog converters (DAC) and analog-to-digital converters (ADC) to operate at the bandwidth which may not be available today or may not be cost-effective. In order to resolve the electronic bandwidth bottleneck associated with DAC/ADC devices, we propose and elucidate the principle of orthogonal-band-multiplexed OFDM (OBM-OFDM) to subdivide the entire OFDM spectrum into multiple orthogonal bands. With this scheme, the DAC/ADCs do not need to operate at extremely high sampling rate. The corresponding mapping to the mixed-signal integrated circuit (IC) design is also revealed. Additionally, we show the proof-of-concept transmission experiment through optical realization of OBM-OFDM. To the best of our knowledge, we present the first experimental demonstration of 107 Gb/s QPSK-encoded CO-OFDM signal transmission over 1000 km standard-single- mode-fiber (SSMF) without optical dispersion compensation and without Raman amplification. The demonstrated system employs 2x2 MIMO-OFDM signal processing and achieves high electrical spectral efficiency with direct-conversion at both transmitter and receiver.

A scheme for recording a fast process at nanosecond scale by using digital holographic interferometry with continuous wave laser

NASA Astrophysics Data System (ADS)

Wang, Jun; Zhao, Jianlin; Di, Jianglei; Jiang, Biqiang

2015-04-01

A scheme for recording fast process at nanosecond scale by using digital holographic interferometry with continuous wave (CW) laser is described and demonstrated experimentally, which employs delayed-time fibers and angular multiplexing technique and can realize the variable temporal resolution at nanosecond scale and different measured depths of object field at certain temporal resolution. The actual delay-time is controlled by two delayed-time fibers with different lengths. The object field information in two different states can be simultaneously recorded in a composite hologram. This scheme is also suitable for recording fast process at picosecond scale, by using an electro-optic modulator.

Development of high precision digital driver of acoustic-optical frequency shifter for ROG

NASA Astrophysics Data System (ADS)

Zhang, Rong; Kong, Mei; Xu, Yameng

2016-10-01

We develop a high precision digital driver of the acoustic-optical frequency shifter (AOFS) based on the parallel direct digital synthesizer (DDS) technology. We use an atomic clock as the phase-locked loop (PLL) reference clock, and the PLL is realized by a dual digital phase-locked loop. A DDS sampling clock up to 320 MHz with a frequency stability as low as 10-12 Hz is obtained. By constructing the RF signal measurement system, it is measured that the frequency output range of the AOFS-driver is 52-58 MHz, the center frequency of the band-pass filter is 55 MHz, the ripple in the band is less than 1 dB@3MHz, the single channel output power is up to 0.3 W, the frequency stability is 1 ppb (1 hour duration), and the frequency-shift precision is 0.1 Hz. The obtained frequency stability has two orders of improvement compared to that of the analog AOFS-drivers. For the designed binary frequency shift keying (2-FSK) and binary phase shift keying (2-PSK) modulation system, the demodulating frequency of the input TTL synchronous level signal is up to 10 kHz. The designed digital-bus coding/decoding system is compatible with many conventional digital bus protocols. It can interface with the ROG signal detecting software through the integrated drive electronics (IDE) and exchange data with the two DDS frequency-shift channels through the signal detecting software.

All-digital phase-lock loops for noise-free signals

NASA Technical Reports Server (NTRS)

Anderson, T. O.

1973-01-01

Bit-synchronizers utilize all-digital phase-lock loops that are referenced to a high frequency digital clock. Phase-lock loop of first design acquires frequency within nominal range and tracks phase; second design is modified for random binary data by addition of simple transition detector; and third design acquires frequency over wide dynamic range.

Magnetics and Power System Upgrades for the Pegasus-U Experiment

NASA Astrophysics Data System (ADS)

Preston, R. C.; Bongard, M. W.; Fonck, R. J.; Lewicki, B. T.

2014-10-01

To support the missions of developing local helicity injection startup and exploiting advanced tokamak physics studies at near unity aspect ratio, the proposed Pegasus-U will include expanded magnetic systems and associated power supplies. A new centerstack increases the toroidal field seven times to 1 T and the volt-seconds by a factor of six while maintaining operation at an aspect ratio of 1.2. The poloidal field magnet system is expanded to support improved shape control and robust double or single null divertor operation at the full plasma current of 0.3 MA. An integrated digital control system based on Field Programmable Gate Arrays (FPGAs) provides active feedback control of all magnet currents. Implementation of the FPGAs is achieved with modular noise reducing electronics. The digital feedback controllers replace the existing analog systems and switch multiplexing technology. This will reduce noise sensitivity and allow the operational Ohmic power supply voltage to increase from 2100 V to its maximum capacity of 2400 V. The feedback controller replacement also allows frequency control for ``freewheeling''--stopping the switching for a short interval and allowing the current to coast. The FPGAs assist in optimizing pulse length by having programmable switching events to minimize energy losses. They also allow for more efficient switching topologies that provide improved stored energy utilization, and support increasing the pulse length from 25 ms to 50-100 ms. Work supported by US DOE Grant DE-FG02-96ER54375.

Adaptation of the Electra Radio to Support Multiple Receive Channels

NASA Technical Reports Server (NTRS)

Satorius, Edgar H.; Shah, Biren N.; Bruvold, Kristoffer N.; Bell, David J.

2011-01-01

Proposed future Mars missions plan communication between multiple assets (rovers). This paper presents the results of a study carried out to assess the potential adaptation of the Electra radio to a multi-channel transceiver. The basic concept is a Frequency Division multiplexing (FDM) communications scheme wherein different receiver architectures are examined. Options considered include: (1) multiple IF slices, A/D and FPGAs each programmed with an Electra baseband modem; (2) common IF but multiple A/Ds and FPGAs and (3) common IF, single A/D and single or multiple FPGAs programmed to accommodate the FDM signals. These options represent the usual tradeoff between analog and digital complexity. Given the space application, a common IF is preferable; however, multiple users present dynamic range challenges (e.g., near-far constraints) that would favor multiple IF slices (Option 1). Vice versa, with a common IF and multiple A/Ds (Option 2), individual AGC control of the A/Ds would be an important consideration. Option 3 would require a common AGC control strategy and would entail multiple digital down conversion paths within the FPGA. In this paper, both FDM parameters as well as the different Electra design options will be examined. In particular, signal channel spacing as a function of user data rates and transmit powers will be evaluated. In addition, tradeoffs between the different Electra design options will be presented with the ultimate goal of defining an augmented Electra radio architecture for potential future missions.

Monolithically Integrated Reconfigurable Filters for Microwave Photonic Links

NASA Astrophysics Data System (ADS)

Norberg, Erik J.

For the purposes of commercial communication and military electronic warfare and radar alike, there is an increasing interest in RF systems that can handle very wide instantaneous bandwidths at high center frequencies. Optical signal processing has the capability to reduce latency, improve size, weight and power (SwAP) performance, and overcome the inherent bandwidth limitations of electronic counterparts. By rapidly pre-filtering wide bandwidth microwave signals in the optical domain, the analog-to-digital conversion (ADC) and subsequent digital signal processing (DSP) can be significantly relieved. Compared to channelizing and add/drop filters for wavelength division multiplexing (WDM) applications, the microwave filter application is much more challenging as it requires a more versatile filter, ideally with tunability in both frequency and bandwidth. In this work such a filter was developed using integrated photonics. By integrating the filter on a single InP chip, the stability required for coherent filtering is met, while the active integration platform offers a flexible filter design and higher tolerance in the coupler and fabrication specifications. Using an entirely deep etched fabrication with a single blanket regrowth, a simple fabrication with high yield is achieved. The reconfigurable filter is designed as an array of uncoupled filter stages with each filter stage reconfigurable as a filter pole or zero with arbitrary magnitude and phase. This gives rise to a flexible ffilter synthesis, much like an optical version of DSP filters. Flat-topped bandpass filters are demonstrated with frequency tunability over 30 GHz, bandwidth adjustable between 1.9 and 5.4 GHz, and stopband rejection >32 dB. In order to meet the stringent spurious-free dynamic range (SFDR) requirements of the microwave application, a novel epitaxial layer integration platform is developed. Optimized for high optical saturation power and low propagation loss, it produces semiconductor optical amplifiers (SOAs) with low distortion and noise. Utilizing a novel characterization method of RF signal distortion for photonic devices, SOAs with state-of-the art SFDR in the range of 115 dB--Hz2/3 and a noise figure of 3.8 dB for 6 dB gain, is demonstrated. It is projected that this platform could ultimately provide integration for photonic microwave filter applications.

Elucidating the Burden of HIV in Tissues Using Multiplexed Immunofluorescence and In Situ Hybridization: Methods for the Single-Cell Phenotypic Characterization of Cells Harboring HIV In Situ.

PubMed

Vasquez, Joshua J; Hussien, Rajaa; Aguilar-Rodriguez, Brandon; Junger, Henrik; Dobi, Dejan; Henrich, Timothy J; Thanh, Cassandra; Gibson, Erica; Hogan, Louise E; McCune, Joseph; Hunt, Peter W; Stoddart, Cheryl A; Laszik, Zoltan G

2018-06-01

Persistent tissue reservoirs of HIV present a major barrier to cure. Defining subsets of infected cells in tissues is a major focus of HIV cure research. Herein, we describe a novel multiplexed in situ hybridization (ISH) (RNAscope) protocol to detect HIV-DNA (vDNA) and HIV-RNA (vRNA) in formalin-fixed paraffin-embedded (FFPE) human tissues in combination with immunofluorescence (IF) phenotyping of the infected cells. We show that multiplexed IF and ISH (mIFISH) is suitable for quantitative assessment of HIV vRNA and vDNA and that multiparameter IF phenotyping allows precise identification of the cellular source of the ISH signal. We also provide semi-quantitative data on the impact of various tissue fixatives on the detectability of vDNA and vRNA with RNAscope technology. Finally, we describe methods to quantitate the ISH signal on whole-slide digital images and validation of the quantitative ISH data with quantitative real-time PCR for vRNA. It is our hope that this approach will provide insight into the biology of HIV tissue reservoirs and to inform strategies aimed at curing HIV.

Simultaneous multiplexing and encoding of multiple images based on a double random phase encryption system

NASA Astrophysics Data System (ADS)

Alfalou, Ayman; Mansour, Ali

2009-09-01

Nowadays, protecting information is a major issue in any transmission system, as showed by an increasing number of research papers related to this topic. Optical encoding methods, such as a Double Random Phase encryption system i.e. DRP, are widely used and cited in the literature. DRP systems have very simple principle and they are easily applicable to most images (B&W, gray levels or color). Moreover, some applications require an enhanced encoding level based on multiencryption scheme and including biometric keys (as digital fingerprints). The enhancement should be done without increasing transmitted or stored information. In order to achieve that goal, a new approach for simultaneous multiplexing & encoding of several target images is developed in this manuscript. By introducing two additional security levels, our approach enhances the security level of a classic "DRP" system. Our first security level consists in using several independent image-keys (randomly and structurally) along with a new multiplexing algorithm. At this level, several target images (multiencryption) are used. This part can reduce needed information (encoding information). At the second level a standard DRP system is included. Finally, our approach can detect if any vandalism attempt has been done on transmitted encrypted images.

Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections.

PubMed

Bolognesi, Maddalena Maria; Manzoni, Marco; Scalia, Carla Rossana; Zannella, Stefano; Bosisio, Francesca Maria; Faretta, Mario; Cattoretti, Giorgio

2017-08-01

Multiplexing, labeling for multiple immunostains in the very same cell or tissue section in situ, has raised considerable interest. The methods proposed include the use of labeled primary antibodies, spectral separation of fluorochromes, bleaching of the fluorophores or chromogens, blocking of previous antibody layers, all in various combinations. The major obstacles to the diffusion of this technique are high costs in custom antibodies and instruments, low throughput, and scarcity of specialized skills or facilities. We have validated a method based on common primary and secondary antibodies and diffusely available fluorescent image scanners. It entails rounds of four-color indirect immunofluorescence, image acquisition, and removal (stripping) of the antibodies, before another stain is applied. The images are digitally registered and the autofluorescence is subtracted. Removal of antibodies is accomplished by disulfide cleavage and a detergent or by a chaotropic salt treatment, this latter followed by antigen refolding. More than 30 different antibody stains can be applied to one single section from routinely fixed and embedded tissue. This method requires a modest investment in hardware and materials and uses freeware image analysis software. Multiplexing on routine tissue sections is a high throughput tool for in situ characterization of neoplastic, reactive, inflammatory, and normal cells.

Investigation of SIS Up-Converters for Use in Multi-pixel Receivers

NASA Astrophysics Data System (ADS)

Uzawa, Yoshinori; Kojima, Takafumi; Shan, Wenlei; Gonzalez, Alvaro; Kroug, Matthias

2018-02-01

We propose the use of SIS junctions as a frequency up-converter based on quasiparticle mixing in frequency division multiplexing circuits for multi-pixel heterodyne receivers. Our theoretical calculation showed that SIS junctions have the potential to achieve positive gain and low-noise characteristics in the frequency up-conversion process at local oscillator (LO) frequencies larger than the voltage scale of the dc nonlinearity of the SIS junction. We experimentally observed up-conversion gain in a mixer with four-series Nb-based SIS junctions at the LO frequency of 105 GHz for the first time.

Impact of MAC Delay on AUV Localization: Underwater Localization Based on Hyperbolic Frequency Modulation Signal

PubMed Central

2018-01-01

Medium Access Control (MAC) delay which occurs between the anchor node’s transmissions is one of the error sources in underwater localization. In particular, in AUV localization, the MAC delay significantly degrades the ranging accuracy. The Cramer-Rao Low Bound (CRLB) definition theoretically proves that the MAC delay significantly degrades the localization performance. This paper proposes underwater localization combined with multiple access technology to decouple the localization performance from the MAC delay. Towards this goal, we adopt hyperbolic frequency modulation (HFM) signal that provides multiplexing based on its good property, high-temporal correlation. Owing to the multiplexing ability of the HFM signal, the anchor nodes can transmit packets without MAC delay, i.e., simultaneous transmission is possible. In addition, the simulation results show that the simultaneous transmission is not an optional communication scheme, but essential for the localization of mobile object in underwater. PMID:29373518

Impact of MAC Delay on AUV Localization: Underwater Localization Based on Hyperbolic Frequency Modulation Signal.

PubMed

Kim, Sungryul; Yoo, Younghwan

2018-01-26

Medium Access Control (MAC) delay which occurs between the anchor node's transmissions is one of the error sources in underwater localization. In particular, in AUV localization, the MAC delay significantly degrades the ranging accuracy. The Cramer-Rao Low Bound (CRLB) definition theoretically proves that the MAC delay significantly degrades the localization performance. This paper proposes underwater localization combined with multiple access technology to decouple the localization performance from the MAC delay. Towards this goal, we adopt hyperbolic frequency modulation (HFM) signal that provides multiplexing based on its good property, high-temporal correlation. Owing to the multiplexing ability of the HFM signal, the anchor nodes can transmit packets without MAC delay, i.e., simultaneous transmission is possible. In addition, the simulation results show that the simultaneous transmission is not an optional communication scheme, but essential for the localization of mobile object in underwater.

The effect of structural design parameters on FPGA-based feed-forward space-time trellis coding-orthogonal frequency division multiplexing channel encoders

NASA Astrophysics Data System (ADS)

Passas, Georgios; Freear, Steven; Fawcett, Darren

2010-08-01

Orthogonal frequency division multiplexing (OFDM)-based feed-forward space-time trellis code (FFSTTC) encoders can be synthesised as very high speed integrated circuit hardware description language (VHDL) designs. Evaluation of their FPGA implementation can lead to conclusions that help a designer to decide the optimum implementation, given the encoder structural parameters. VLSI architectures based on 1-bit multipliers and look-up tables (LUTs) are compared in terms of FPGA slices and block RAMs (area), as well as in terms of minimum clock period (speed). Area and speed graphs versus encoder memory order are provided for quadrature phase shift keying (QPSK) and 8 phase shift keying (8-PSK) modulation and two transmit antennas, revealing best implementation under these conditions. The effect of number of modulation bits and transmit antennas on the encoder implementation complexity is also investigated.

Energy-efficient orthogonal frequency division multiplexing-based passive optical network based on adaptive sleep-mode control and dynamic bandwidth allocation

NASA Astrophysics Data System (ADS)

Zhang, Chongfu; Xiao, Nengwu; Chen, Chen; Yuan, Weicheng; Qiu, Kun

2016-02-01

We propose an energy-efficient orthogonal frequency division multiplexing-based passive optical network (OFDM-PON) using adaptive sleep-mode control and dynamic bandwidth allocation. In this scheme, a bidirectional-centralized algorithm named the receiver and transmitter accurate sleep control and dynamic bandwidth allocation (RTASC-DBA), which has an overall bandwidth scheduling policy, is employed to enhance the energy efficiency of the OFDM-PON. The RTASC-DBA algorithm is used in an optical line terminal (OLT) to control the sleep mode of an optical network unit (ONU) sleep and guarantee the quality of service of different services of the OFDM-PON. The obtained results show that, by using the proposed scheme, the average power consumption of the ONU is reduced by ˜40% when the normalized ONU load is less than 80%, compared with the average power consumption without using the proposed scheme.

Image secure transmission for optical orthogonal frequency-division multiplexing visible light communication systems using chaotic discrete cosine transform

NASA Astrophysics Data System (ADS)

Wang, Zhongpeng; Zhang, Shaozhong; Chen, Fangni; Wu, Ming-Wei; Qiu, Weiwei

2017-11-01

A physical encryption scheme for orthogonal frequency-division multiplexing (OFDM) visible light communication (VLC) systems using chaotic discrete cosine transform (DCT) is proposed. In the scheme, the row of the DCT matrix is permutated by a scrambling sequence generated by a three-dimensional (3-D) Arnold chaos map. Furthermore, two scrambling sequences, which are also generated from a 3-D Arnold map, are employed to encrypt the real and imaginary parts of the transmitted OFDM signal before the chaotic DCT operation. The proposed scheme enhances the physical layer security and improves the bit error rate (BER) performance for OFDM-based VLC. The simulation results prove the efficiency of the proposed encryption method. The experimental results show that the proposed security scheme not only protects image data from eavesdroppers but also keeps the good BER and peak-to-average power ratio performances for image-based OFDM-VLC systems.

Optical wireless communication using positive real-valued orthogonal frequency-division multiplexing and optical beamforming

NASA Astrophysics Data System (ADS)

Kim, Sung-Man; Kwon, Ki-Keun

2017-07-01

The relatively unsatisfactory performance of optical wireless communication (OWC) with respect to WiFi and millimeter-wave communications has formed a key issue preventing its commercialization. We experimentally demonstrate an OWC technology using a combination of positive real-valued orthogonal frequency-division multiplexing (OFDM) and optical beamforming (OB). Due to the intensity-modulation and direct-detection aspects of OWC systems, a positive real-valued OFDM signal can be suitably utilized to maximize the OWC data rate. Further, the OB technique, which can focus laser light on a desired target, can be utilized to increase the OWC data rate and transmission distance. Our experimental results show that the received optical signal power and electrical signal increase by up to 42 and 25 dB, respectively. Further, the data rate increases by a factor of 200 with OB over the conventional approach.

Performance Analysis of Diversity-Controlled Multi-User Superposition Transmission for 5G Wireless Networks

PubMed Central

Yeom, Jeong Seon; Jung, Bang Chul; Jin, Hu

2018-01-01

In this paper, we propose a novel low-complexity multi-user superposition transmission (MUST) technique for 5G downlink networks, which allows multiple cell-edge users to be multiplexed with a single cell-center user. We call the proposed technique diversity-controlled MUST technique since the cell-center user enjoys the frequency diversity effect via signal repetition over multiple orthogonal frequency division multiplexing (OFDM) sub-carriers. We assume that a base station is equipped with a single antenna but users are equipped with multiple antennas. In addition, we assume that the quadrature phase shift keying (QPSK) modulation is used for users. We mathematically analyze the bit error rate (BER) of both cell-edge users and cell-center users, which is the first theoretical result in the literature to the best of our knowledge. The mathematical analysis is validated through extensive link-level simulations. PMID:29439413

TES-Based X-Ray Microcalorimeter Performances Under AC Bias and FDM for Athena

NASA Technical Reports Server (NTRS)

Akamatsu, H.; Gottardi, L.; de Vries, C. P.; Adams, J. S.; Bandler, S. R.; Bruijn, M. P.; Chervenak, J. A.; Eckart, M. E.; Finkbeiner, F. M.; Gao, J. R.;

Performance Analysis of Diversity-Controlled Multi-User Superposition Transmission for 5G Wireless Networks.

PubMed

Yeom, Jeong Seon; Chu, Eunmi; Jung, Bang Chul; Jin, Hu

2018-02-10

In this paper, we propose a novel low-complexity multi-user superposition transmission (MUST) technique for 5G downlink networks, which allows multiple cell-edge users to be multiplexed with a single cell-center user. We call the proposed technique diversity-controlled MUST technique since the cell-center user enjoys the frequency diversity effect via signal repetition over multiple orthogonal frequency division multiplexing (OFDM) sub-carriers. We assume that a base station is equipped with a single antenna but users are equipped with multiple antennas. In addition, we assume that the quadrature phase shift keying (QPSK) modulation is used for users. We mathematically analyze the bit error rate (BER) of both cell-edge users and cell-center users, which is the first theoretical result in the literature to the best of our knowledge. The mathematical analysis is validated through extensive link-level simulations.

Increasing throughput of multiplexed electrical bus in pipe-lined architecture

DOEpatents

Asaad, Sameh; Brezzo, Bernard V; Kapur, Mohit

2014-05-27

Techniques are disclosed for increasing the throughput of a multiplexed electrical bus by exploiting available pipeline stages of a computer or other system. For example, a method for increasing a throughput of an electrical bus that connects at least two devices in a system comprises introducing at least one signal hold stage in a signal-receiving one of the two devices, such that a maximum frequency at which the two devices are operated is not limited by a number of cycles of an operating frequency of the electrical bus needed for a signal to propagate from a signal-transmitting one of the two devices to the signal-receiving one of the two devices. Preferably, the signal hold stage introduced in the signal-receiving one of the two devices is a pipeline stage re-allocated from the signal-transmitting one of the two devices.