Digital Speckle Photography of Subpixel Displacements of Speckle Structures Based on Analysis of Their Spatial Spectra

NASA Astrophysics Data System (ADS)

Maksimova, L. A.; Ryabukho, P. V.; Mysina, N. Yu.; Lyakin, D. V.; Ryabukho, V. P.

2018-04-01

We have investigated the capabilities of the method of digital speckle interferometry for determining subpixel displacements of a speckle structure formed by a displaceable or deformable object with a scattering surface. An analysis of spatial spectra of speckle structures makes it possible to perform measurements with a subpixel accuracy and to extend the lower boundary of the range of measurements of displacements of speckle structures to the range of subpixel values. The method is realized on the basis of digital recording of the images of undisplaced and displaced speckle structures, their spatial frequency analysis using numerically specified constant phase shifts, and correlation analysis of spatial spectra of speckle structures. Transformation into the frequency range makes it possible to obtain quantities to be measured with a subpixel accuracy from the shift of the interference-pattern minimum in the diffraction halo by introducing an additional phase shift into the complex spatial spectrum of the speckle structure or from the slope of the linear plot of the function of accumulated phase difference in the field of the complex spatial spectrum of the displaced speckle structure. The capabilities of the method have been investigated in natural experiment.

Differential high-speed digital micromirror device based fluorescence speckle confocal microscopy.

PubMed

Jiang, Shihong; Walker, John

2010-01-20

We report a differential fluorescence speckle confocal microscope that acquires an image in a fraction of a second by exploiting the very high frame rate of modern digital micromirror devices (DMDs). The DMD projects a sequence of predefined binary speckle patterns to the sample and modulates the intensity of the returning fluorescent light simultaneously. The fluorescent light reflecting from the DMD's "on" and "off" pixels is modulated by correlated speckle and anticorrelated speckle, respectively, to form two images on two CCD cameras in parallel. The sum of the two images recovers a widefield image, but their difference gives a near-confocal image in real time. Experimental results for both low and high numerical apertures are shown.

Retrieval of phase-derivative discontinuities in digital speckle pattern interferometry fringes using the Wigner-Ville distribution

NASA Astrophysics Data System (ADS)

Federico, Alejandro; Kaufmann, Guillermo H.

2004-08-01

We evaluate the application of the Wigner-Ville distribution (WVD) to measure phase gradient maps in digital speckle pattern interferometry (DSPI), when the generated correlation fringes present phase discontinuities. The performance of the WVD method is evaluated using computer-simulated fringes. The influence of the filtering process to smooth DSPI fringes and additional drawbacks that emerge when this method is applied are discussed. A comparison with the conventional method based on the continuous wavelet transform in the stationary phase approximation is also presented.

Simulation of speckle patterns with pre-defined correlation distributions.

PubMed

Song, Lipei; Zhou, Zhen; Wang, Xueyan; Zhao, Xing; Elson, Daniel S

2016-03-01

We put forward a method to easily generate a single or a sequence of fully developed speckle patterns with pre-defined correlation distribution by utilizing the principle of coherent imaging. The few-to-one mapping between the input correlation matrix and the correlation distribution between simulated speckle patterns is realized and there is a simple square relationship between the values of these two correlation coefficient sets. This method is demonstrated both theoretically and experimentally. The square relationship enables easy conversion from any desired correlation distribution. Since the input correlation distribution can be defined by a digital matrix or a gray-scale image acquired experimentally, this method provides a convenient way to simulate real speckle-related experiments and to evaluate data processing techniques.

Simulation of speckle patterns with pre-defined correlation distributions

PubMed Central

Song, Lipei; Zhou, Zhen; Wang, Xueyan; Zhao, Xing; Elson, Daniel S.

2016-01-01

We put forward a method to easily generate a single or a sequence of fully developed speckle patterns with pre-defined correlation distribution by utilizing the principle of coherent imaging. The few-to-one mapping between the input correlation matrix and the correlation distribution between simulated speckle patterns is realized and there is a simple square relationship between the values of these two correlation coefficient sets. This method is demonstrated both theoretically and experimentally. The square relationship enables easy conversion from any desired correlation distribution. Since the input correlation distribution can be defined by a digital matrix or a gray-scale image acquired experimentally, this method provides a convenient way to simulate real speckle-related experiments and to evaluate data processing techniques. PMID:27231589

Simultaneous acquisition of 3D shape and deformation by combination of interferometric and correlation-based laser speckle metrology.

PubMed

Dekiff, Markus; Berssenbrügge, Philipp; Kemper, Björn; Denz, Cornelia; Dirksen, Dieter

2015-12-01

A metrology system combining three laser speckle measurement techniques for simultaneous determination of 3D shape and micro- and macroscopic deformations is presented. While microscopic deformations are determined by a combination of Digital Holographic Interferometry (DHI) and Digital Speckle Photography (DSP), macroscopic 3D shape, position and deformation are retrieved by photogrammetry based on digital image correlation of a projected laser speckle pattern. The photogrammetrically obtained data extend the measurement range of the DHI-DSP system and also increase the accuracy of the calculation of the sensitivity vector. Furthermore, a precise assignment of microscopic displacements to the object's macroscopic shape for enhanced visualization is achieved. The approach allows for fast measurements with a simple setup. Key parameters of the system are optimized, and its precision and measurement range are demonstrated. As application examples, the deformation of a mandible model and the shrinkage of dental impression material are measured.

Color quality improvement of reconstructed images in color digital holography using speckle method and spectral estimation

NASA Astrophysics Data System (ADS)

Funamizu, Hideki; Onodera, Yusei; Aizu, Yoshihisa

2018-05-01

In this study, we report color quality improvement of reconstructed images in color digital holography using the speckle method and the spectral estimation. In this technique, an object is illuminated by a speckle field and then an object wave is produced, while a plane wave is used as a reference wave. For three wavelengths, the interference patterns of two coherent waves are recorded as digital holograms on an image sensor. Speckle fields are changed by moving a ground glass plate in an in-plane direction, and a number of holograms are acquired to average the reconstructed images. After the averaging process of images reconstructed from multiple holograms, we use the Wiener estimation method for obtaining spectral transmittance curves in reconstructed images. The color reproducibility in this method is demonstrated and evaluated using a Macbeth color chart film and staining cells of onion.

Sound recovery via intensity variations of speckle pattern pixels selected with variance-based method

NASA Astrophysics Data System (ADS)

Zhu, Ge; Yao, Xu-Ri; Qiu, Peng; Mahmood, Waqas; Yu, Wen-Kai; Sun, Zhi-Bin; Zhai, Guang-Jie; Zhao, Qing

2018-02-01

In general, the sound waves can cause the vibration of the objects that are encountered in the traveling path. If we make a laser beam illuminate the rough surface of an object, it will be scattered into a speckle pattern that vibrates with these sound waves. Here, an efficient variance-based method is proposed to recover the sound information from speckle patterns captured by a high-speed camera. This method allows us to select the proper pixels that have large variances of the gray-value variations over time, from a small region of the speckle patterns. The gray-value variations of these pixels are summed together according to a simple model to recover the sound with a high signal-to-noise ratio. Meanwhile, our method will significantly simplify the computation compared with the traditional digital-image-correlation technique. The effectiveness of the proposed method has been verified by applying a variety of objects. The experimental results illustrate that the proposed method is robust to the quality of the speckle patterns and costs more than one-order less time to perform the same number of the speckle patterns. In our experiment, a sound signal of time duration 1.876 s is recovered from various objects with time consumption of 5.38 s only.

A polarized digital shearing speckle pattern interferometry system based on temporal wavelet transformation.

PubMed

Feng, Ziang; Gao, Zhan; Zhang, Xiaoqiong; Wang, Shengjia; Yang, Dong; Yuan, Hao; Qin, Jie

2015-09-01

Digital shearing speckle pattern interferometry (DSSPI) has been recognized as a practical tool in testing strain. The DSSPI system which is based on temporal analysis is attractive because of its ability to measure strain dynamically. In this paper, such a DSSPI system with Wollaston prism has been built. The principles and system arrangement are described and the preliminary experimental result of the displacement-derivative test of an aluminum plate is shown with the wavelet transformation method and the Fourier transformation method. The simulations have been conducted with the finite element method. The comparison of the results shows that quantitative measurement of displacement-derivative has been realized.

Speckle-field digital holographic microscopy.

PubMed

Park, YongKeun; Choi, Wonshik; Yaqoob, Zahid; Dasari, Ramachandra; Badizadegan, Kamran; Feld, Michael S

2009-07-20

The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. Here, we report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging. A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

Direct Measurement of Large, Diffuse, Optical Structures

NASA Technical Reports Server (NTRS)

Saif, Babak N.; Keski-Kuha, Ritva; Feinberg, Lee; Wyant, J. C.; Atkinson, C.

2004-01-01

Digital Speckle Pattern Interferometry (DSPI) is a well-established method for the measurement of diffuse objects in experimental mechanics. DSPIs are phase shifting interferometers. Three or four bucket temporal phase shifting algorithms are commonly used to provide phase shifting. These algorithms are sensitive to vibrations and can not be used to measure large optical structures far away from the interferometer. In this research a simultaneous phase shifted interferometer, PhaseCam product of 4D Technology Corporation in Tucson Arizona, is modified to be a Simultaneous phase shifted Digital Speckle Pattern Interferometer (SDSPI). Repeatability, dynamic range, and accuracy of the SDSPI are characterized by measuring a 5 cm x 5 cm carbon fiber coupon.

Measurement of in-plane displacements using the phase singularities generated by directional wavelet transforms of speckle pattern images.

PubMed

Vadnjal, Ana Laura; Etchepareborda, Pablo; Federico, Alejandro; Kaufmann, Guillermo H

2013-03-20

We present a method to determine micro and nano in-plane displacements based on the phase singularities generated by application of directional wavelet transforms to speckle pattern images. The spatial distribution of the obtained phase singularities by the wavelet transform configures a network, which is characterized by two quasi-orthogonal directions. The displacement value is determined by identifying the intersection points of the network before and after the displacement produced by the tested object. The performance of this method is evaluated using simulated speckle patterns and experimental data. The proposed approach is compared with the optical vortex metrology and digital image correlation methods in terms of performance and noise robustness, and the advantages and limitations associated to each method are also discussed.

Quantitative assessment of soft tissue deformation using digital speckle pattern interferometry: studies on phantom breast models.

PubMed

Karuppanan, Udayakumar; Unni, Sujatha Narayanan; Angarai, Ganesan R

2017-01-01

Assessment of mechanical properties of soft matter is a challenging task in a purely noninvasive and noncontact environment. As tissue mechanical properties play a vital role in determining tissue health status, such noninvasive methods offer great potential in framing large-scale medical screening strategies. The digital speckle pattern interferometry (DSPI)-based image capture and analysis system described here is capable of extracting the deformation information from a single acquired fringe pattern. Such a method of analysis would be required in the case of the highly dynamic nature of speckle patterns derived from soft tissues while applying mechanical compression. Soft phantoms mimicking breast tissue optical and mechanical properties were fabricated and tested in the DSPI out of plane configuration set up. Hilbert transform (HT)-based image analysis algorithm was developed to extract the phase and corresponding deformation of the sample from a single acquired fringe pattern. The experimental fringe contours were found to correlate with numerically simulated deformation patterns of the sample using Abaqus finite element analysis software. The extracted deformation from the experimental fringe pattern using the HT-based algorithm is compared with the deformation value obtained using numerical simulation under similar conditions of loading and the results are found to correlate with an average %error of 10. The proposed method is applied on breast phantoms fabricated with included subsurface anomaly mimicking cancerous tissue and the results are analyzed.

Measurement potential of laser speckle velocimetry

NASA Technical Reports Server (NTRS)

Adrian, R. J.

1982-01-01

Laser speckle velocimetry, the measurement of fluid velocity by measuring the translation of speckle pattern or individual particles that are moving with the fluid, is described. The measurement is accomplished by illuminating the fluid with consecutive pulses of Laser Light and recording the images of the particles or the speckles on a double exposed photographic plate. The plate contains flow information throughout the image plane so that a single double exposure may provide data at hundreds or thousands of points in the illuminated region of the fluid. Conventional interrogation of the specklegram involves illuminating the plate to form Young's fringes, whose spacing is inversely proportional to the speckle separation. Subsequently the fringes are digitized and analyzed in a computer to determine their frequency and orientation, yielding the velocity magnitude and orientation. The Young's fringe technique is equivalent to performing a 2-D spatial correlation of the double exposed specklegram intensity pattern, and this observation suggests that correlation should be considered as an alternative processing method. The principle of the correlation technique is examined.

Holographic interferometric and correlation-based laser speckle metrology for 3D deformations in dentistry

NASA Astrophysics Data System (ADS)

Dekiff, Markus; Kemper, Björn; Kröger, Elke; Denz, Cornelia; Dirksen, Dieter

2017-03-01

The mechanical loading of dental restorations and hard tissue is often investigated numerically. For validation and optimization of such simulations, comparisons with measured deformations are essential. We combine digital holographic interferometry and digital speckle photography for the determination of microscopic deformations with a photogrammetric method that is based on digital image correlation of a projected laser speckle pattern. This multimodal workstation allows the simultaneous acquisition of the specimen's macroscopic 3D shape and thus a quantitative comparison of measured deformations with simulation data. In order to demonstrate the feasibility of our system, two applications are presented: the quantitative determination of (1) the deformation of a mandible model due to mechanical loading of an inserted dental implant and of (2) the deformation of a (dental) bridge model under mechanical loading. The results were compared with data from finite element analyses of the investigated applications. The experimental results showed close agreement with those of the simulations.

Using the auxiliary camera for system calibration of 3D measurement by digital speckle

NASA Astrophysics Data System (ADS)

Xue, Junpeng; Su, Xianyu; Zhang, Qican

2014-06-01

The study of 3D shape measurement by digital speckle temporal sequence correlation have drawn a lot of attention by its own advantages, however, the measurement mainly for depth z-coordinate, horizontal physical coordinate (x, y) are usually marked as image pixel coordinate. In this paper, a new approach for the system calibration is proposed. With an auxiliary camera, we made up the temporary binocular vision system, which are used for the calibration of horizontal coordinates (mm) while the temporal sequence reference-speckle-sets are calibrated. First, the binocular vision system has been calibrated using the traditional method. Then, the digital speckles are projected on the reference plane, which is moved by equal distance in the direction of depth, temporal sequence speckle images are acquired with camera as reference sets. When the reference plane is in the first position and final position, crossed fringe pattern are projected to the plane respectively. The control points of pixel coordinates are extracted by Fourier analysis from the images, and the physical coordinates are calculated by the binocular vision. The physical coordinates corresponding to each pixel of the images are calculated by interpolation algorithm. Finally, the x and y corresponding to arbitrary depth value z are obtained by the geometric formula. Experiments prove that our method can fast and flexibly measure the 3D shape of an object as point cloud.

Quantitative assessment of soft tissue deformation using digital speckle pattern interferometry: studies on phantom breast models

PubMed Central

Karuppanan, Udayakumar; Unni, Sujatha Narayanan; Angarai, Ganesan R.

2017-01-01

Abstract. Assessment of mechanical properties of soft matter is a challenging task in a purely noninvasive and noncontact environment. As tissue mechanical properties play a vital role in determining tissue health status, such noninvasive methods offer great potential in framing large-scale medical screening strategies. The digital speckle pattern interferometry (DSPI)–based image capture and analysis system described here is capable of extracting the deformation information from a single acquired fringe pattern. Such a method of analysis would be required in the case of the highly dynamic nature of speckle patterns derived from soft tissues while applying mechanical compression. Soft phantoms mimicking breast tissue optical and mechanical properties were fabricated and tested in the DSPI out of plane configuration set up. Hilbert transform (HT)-based image analysis algorithm was developed to extract the phase and corresponding deformation of the sample from a single acquired fringe pattern. The experimental fringe contours were found to correlate with numerically simulated deformation patterns of the sample using Abaqus finite element analysis software. The extracted deformation from the experimental fringe pattern using the HT-based algorithm is compared with the deformation value obtained using numerical simulation under similar conditions of loading and the results are found to correlate with an average %error of 10. The proposed method is applied on breast phantoms fabricated with included subsurface anomaly mimicking cancerous tissue and the results are analyzed. PMID:28180134

Full-field 3D deformation measurement: comparison between speckle phase and displacement evaluation.

PubMed

Khodadad, Davood; Singh, Alok Kumar; Pedrini, Giancarlo; Sjödahl, Mikael

2016-09-20

The objective of this paper is to describe a full-field deformation measurement method based on 3D speckle displacements. The deformation is evaluated from the slope of the speckle displacement function that connects the different reconstruction planes. For our experiment, a symmetrical arrangement with four illuminations parallel to the planes (x,z) and (y,z) was used. Four sets of speckle patterns were sequentially recorded by illuminating an object from the four directions, respectively. A single camera is used to record the holograms before and after deformations. Digital speckle photography is then used to calculate relative speckle displacements in each direction between two numerically propagated planes. The 3D speckle displacements vector is calculated as a combination of the speckle displacements from the holograms recorded in each illumination direction. Using the speckle displacements, problems associated with rigid body movements and phase wrapping are avoided. In our experiment, the procedure is shown to give the theoretical accuracy of 0.17 pixels yielding the accuracy of 2×10^-3 in the measurement of deformation gradients.

Fast image processing with a microcomputer applied to speckle photography

NASA Astrophysics Data System (ADS)

Erbeck, R.

1985-11-01

An automated image recognition system is described for speckle photography investigations in fluid dynamics. The system is employed for characterizing the pattern of interference fringes obtained using speckle interferometry. A rotating ground glass serves as a screen on which laser light passing through a specklegraph plate, the flow and a compensation plate (CP) is shone to produce a compensated Young's pattern. The image produced on the ground glass is photographed by a video camera whose signal is digitized and processed through a microcomputer using a 6502 CPU chip. The normalized correlation function of the intensity is calculated in two directions of the recorded pattern to obtain the wavelength and the light deflection angle. The system has a capability of one picture every two seconds. Sample data are provided for a free jet of CO2 issuing into air in both laminar and turbulent form.

Egg Speckling Patterns Do Not Advertise Offspring Quality or Influence Male Provisioning in Great Tits

PubMed Central

Stoddard, Mary Caswell; Fayet, Annette L.; Kilner, Rebecca M.; Hinde, Camilla A.

2012-01-01

Many passerine birds lay white eggs with reddish brown speckles produced by protoporphyrin pigment. However, the function of these spots is contested. Recently, the sexually selected eggshell coloration (SSEC) hypothesis proposed that eggshell color is a sexually selected signal through which a female advertises her quality (and hence the potential quality of her future young) to her male partner, thereby encouraging him to contribute more to breeding attempts. We performed a test of the SSEC hypothesis in a common passerine, the great tit Parus major. We used a double cross-fostering design to determine whether males change their provisioning behavior based on eggshell patterns they observe at the nest. We also tested the assumption that egg patterning reflects female and/or offspring quality. Because birds differ from humans in their color and pattern perception, we used digital photography and models of bird vision to quantify egg patterns objectively. Neither male provisioning nor chick growth was related to the pattern of eggs males observed during incubation. Although heavy females laid paler, less speckled eggs, these eggs did not produce chicks that grew faster. Therefore, we conclude that the SSEC hypothesis is an unlikely explanation for the evolution of egg speckling in great tits. PMID:22815730

Study of nanometer-level precise phase-shift system used in electronic speckle shearography and phase-shift pattern interferometry

NASA Astrophysics Data System (ADS)

Jing, Chao; Liu, Zhongling; Zhou, Ge; Zhang, Yimo

2011-11-01

The nanometer-level precise phase-shift system is designed to realize the phase-shift interferometry in electronic speckle shearography pattern interferometry. The PZT is used as driving component of phase-shift system and translation component of flexure hinge is developed to realize micro displacement of non-friction and non-clearance. Closed-loop control system is designed for high-precision micro displacement, in which embedded digital control system is developed for completing control algorithm and capacitive sensor is used as feedback part for measuring micro displacement in real time. Dynamic model and control model of the nanometer-level precise phase-shift system is analyzed, and high-precision micro displacement is realized with digital PID control algorithm on this basis. It is proved with experiments that the location precision of the precise phase-shift system to step signal of displacement is less than 2nm and the location precision to continuous signal of displacement is less than 5nm, which is satisfied with the request of the electronic speckle shearography and phase-shift pattern interferometry. The stripe images of four-step phase-shift interferometry and the final phase distributed image correlated with distortion of objects are listed in this paper to prove the validity of nanometer-level precise phase-shift system.

A decade of innovation with laser speckle metrology

NASA Astrophysics Data System (ADS)

Ettemeyer, Andreas

2003-05-01

Speckle Pattern Interferometry has emerged from the experimental substitution of holographic interferometry to become a powerful problem solving tool in research and industry. The rapid development of computer and digital imaging techniques in combination with minaturization of the optical equipment led to new applications which had not been anticipated before. While classical holographic interferometry had always required careful consideration of the environmental conditions such as vibration, noise, light, etc. and could generally only be performed in the optical laboratory, it is now state of the art, to handle portable speckle measuring equipment at almost any place. During the last decade, the change in design and technique has dramatically influenced the range of applications of speckle metrology and opened new markets. The integration of recent research results into speckle measuring equipment has led to handy equipment, simplified the operation and created high quality data output.

Phase retrieval in digital speckle pattern interferometry by application of two-dimensional active contours called snakes.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2006-03-20

We propose a novel approach to retrieving the phase map coded by a single closed-fringe pattern in digital speckle pattern interferometry, which is based on the estimation of the local sign of the quadrature component. We obtain the estimate by calculating the local orientation of the fringes that have previously been denoised by a weighted smoothing spline method. We carry out the procedure of sign estimation by determining the local abrupt jumps of size pi in the orientation field of the fringes and by segmenting the regions defined by these jumps. The segmentation method is based on the application of two-dimensional active contours (snakes), with which one can also estimate absent jumps, i.e., those that cannot be detected from the local orientation of the fringes. The performance of the proposed phase-retrieval technique is evaluated for synthetic and experimental fringes and compared with the results obtained with the spiral-phase- and Fourier-transform methods.

Phase retrieval in digital speckle pattern interferometry by use of a smoothed space-frequency distribution.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2003-12-10

We evaluate the use of a smoothed space-frequency distribution (SSFD) to retrieve optical phase maps in digital speckle pattern interferometry (DSPI). The performance of this method is tested by use of computer-simulated DSPI fringes. Phase gradients are found along a pixel path from a single DSPI image, and the phase map is finally determined by integration. This technique does not need the application of a phase unwrapping algorithm or the introduction of carrier fringes in the interferometer. It is shown that a Wigner-Ville distribution with a smoothing Gaussian kernel gives more-accurate results than methods based on the continuous wavelet transform. We also discuss the influence of filtering on smoothing of the DSPI fringes and some additional limitations that emerge when this technique is applied. The performance of the SSFD method for processing experimental data is then illustrated.

Digital imaging information technology for biospeckle activity assessment relative to bacteria and parasites.

PubMed

Ramírez-Miquet, Evelio E; Cabrera, Humberto; Grassi, Hilda C; de J Andrades, Efrén; Otero, Isabel; Rodríguez, Dania; Darias, Juan G

2017-08-01

This paper reports on the biospeckle processing of biological activity using a visualization scheme based upon the digital imaging information technology. Activity relative to bacterial growth in agar plates and to parasites affected by a drug is monitored via the speckle patterns generated by a coherent source incident on the microorganisms. We present experimental results to demonstrate the potential application of this methodology for following the activity in time. The digital imaging information technology is an alternative visualization enabling the study of speckle dynamics, which is correlated to the activity of bacteria and parasites. In this method, the changes in Red-Green-Blue (RGB) color component density are considered as markers of the growth of bacteria and parasites motility in presence of a drug. The RGB data was used to generate a two-dimensional surface plot allowing an analysis of color distribution on the speckle images. The proposed visualization is compared to the outcomes of the generalized differences and the temporal difference. A quantification of the activity is performed using a parameterization of the temporal difference method. The adopted digital image processing technique has been found suitable to monitor motility and morphological changes in the bacterial population over time and to detect and distinguish a short term drug action on parasites.

An in vivo analysis of facial muscle change treated with botulinum toxin type A using digital image speckle correlation

NASA Astrophysics Data System (ADS)

Xu, Yan; Palmaccio, Samantha Palmaccio; Bui, Duc; Dagum, Alexander; Rafailovich, Miriam

Been famous for clinical use from early 1980s, the neuromuscular blocking agent Botulinum toxin type A (BTX-A), has been used to reduce wrinkles for a long time. Only little research has been done to quantify the change of muscle contraction before and after injection and most research paper depend on subjective evaluation from both patients and surgeons. In our research, Digital Image Speckle Correlation (DISC) was employed to study the mechanical properties of skin, contraction mode of muscles (injected) and reaction of neighbor muscle group (un-injected).At the same time, displacement patterns (vector maps)generated by DISC can predict injection locus for surgeons who normally handle it depending only on visual observation.

Digital Holographic Interferometry and Speckle Correlation

NASA Astrophysics Data System (ADS)

Yamaguchi, Ichirou

2010-04-01

Relations and combinations between holographic interferometry and speckle correlation in contouring by phase-shifting digital holography are discussed. Three-dimensional distributions of correlations of the complex amplitudes and intensities before and after the laser wavelength shift are calculated in numerical simulations where a rough surface is modeled with random numbers. Fringe localization related to speckle displacement as well as speckle suppression in phase analysis are demonstrated for general surface shape and recording conditions.

Digital speckle correlation for nondestructive testing of corrosion

NASA Astrophysics Data System (ADS)

Paiva, Raul D., Jr.; Soga, Diogo; Muramatsu, Mikiya; Hogert, Elsa N.; Landau, Monica R.; Ruiz Gale, Maria F.; Gaggioli, Nestor G.

1999-07-01

This paper describes the use of optical correlation speckle patterns to detect and analyze the metallic corrosion phenomena, and shows the experimental set-up used. We present some new results in the characterization of the corrosion process using a model based in electroerosion phenomena. We also provide valuable information about surface microrelief changes, which is also useful in numerous engineering applications. The results obtained are good enough for showing that our technique is very useful for giving new possibilities to the analysis of the corrosion and oxidation process, particularly in real time.

Experimental evaluation of neural probe’s insertion induced injury based on digital image correlation method

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Wenguang, E-mail: zhwg@sjtu.edu.cn; Ma, Yakun; Li, Zhengwei

Purpose: The application of neural probes in clinic has been challenged by probes’ short lifetime when implanted into brain tissue. The primary goal is to develop an evaluation system for testing brain tissue injury induced by neural probe’s insertion using microscope based digital image correlation method. Methods: A brain tissue phantom made of silicone rubber with speckle pattern on its surface was fabricated. To obtain the optimal speckle pattern, mean intensity gradient parameter was used for quality assessment. The designed testing system consists of three modules: (a) load module for simulating neural electrode implantation process; (b) data acquisition module tomore » capture micrographs of speckle pattern and to obtain reactive forces during the insertion of the probe; (c) postprocessing module for extracting tissue deformation information from the captured speckle patterns. On the basis of the evaluation system, the effects of probe wedge angle, insertion speed, and probe streamline on insertion induced tissue injury were investigated. Results: The optimal quality speckle pattern can be attained by the following fabrication parameters: spin coating rate—1000 r/min, silicone rubber component A: silicone rubber component B: softener: graphite = 5 ml: 5 ml: 2 ml: 0.6 g. The probe wedge angle has a significant effect on tissue injury. Compared to wedge angle 40° and 20°, maximum principal strain of 60° wedge angle was increased by 40.3% and 87.5%, respectively; compared with a relatively higher speed (500 μm/s), the maximum principle strain within the tissue induced by slow insertion speed (100 μm/s) was increased by 14.3%; insertion force required by probe with convex streamline was smaller than the force of traditional probe. Based on the experimental results, a novel neural probe that has a rounded tip covered by a biodegradable silk protein coating with convex streamline was proposed, which has both lower insertion and micromotion induced tissue injury. Conclusions: The established evaluation system has provided a simulation environment for testing brain tissue injury produced by various insertion conditions. At the same time, it eliminates the adverse effect of biological factors on tissue deformation during the experiment, improving the repeatability of measurement results. As a result, the evaluation system will provide support on novel neural probe design that can reduce the acute tissue injury during the implantation of the probe.« less

Single-shot speckle reduction in numerical reconstruction of digitally recorded holograms: comment.

PubMed

Maycock, Jonathan; Hennelly, Bryan; McDonald, John

2015-09-01

We comment on a recent Letter by Hincapie et al. [Opt. Lett.40, 1623 (2015)], in which the authors proposed a method to reduce the speckle noise in digital holograms. This method was previously published by us in Maycock ["Improving reconstructions of digital holograms," Ph.D. thesis (National University of Ireland, 2012)] and Maycock and Hennelly [Improving Reconstructions of Digital Holograms: Speckle Reduction and Occlusions in Digital Holography (Lambert Academic, 2014)]. We also wish to highlight an important limitation of the method resulting from the superposition of different perspectives of the object/scene, which was not addressed in their Letter.

Single shot laser speckle based 3D acquisition system for medical applications

NASA Astrophysics Data System (ADS)

Khan, Danish; Shirazi, Muhammad Ayaz; Kim, Min Young

2018-06-01

The state of the art techniques used by medical practitioners to extract the three-dimensional (3D) geometry of different body parts requires a series of images/frames such as laser line profiling or structured light scanning. Movement of the patients during scanning process often leads to inaccurate measurements due to sequential image acquisition. Single shot structured techniques are robust to motion but the prevalent challenges in single shot structured light methods are the low density and algorithm complexity. In this research, a single shot 3D measurement system is presented that extracts the 3D point cloud of human skin by projecting a laser speckle pattern using a single pair of images captured by two synchronized cameras. In contrast to conventional laser speckle 3D measurement systems that realize stereo correspondence by digital correlation of projected speckle patterns, the proposed system employs KLT tracking method to locate the corresponding points. The 3D point cloud contains no outliers and sufficient quality of 3D reconstruction is achieved. The 3D shape acquisition of human body parts validates the potential application of the proposed system in the medical industry.

Motion detection using extended fractional Fourier transform and digital speckle photography.

PubMed

Bhaduri, Basanta; Tay, C J; Quan, C; Sheppard, Colin J R

2010-05-24

Digital speckle photography is a useful tool for measuring the motion of optically rough surfaces from the speckle shift that takes place at the recording plane. A simple correlation based digital speckle photographic system has been proposed that implements two simultaneous optical extended fractional Fourier transforms (EFRTs) of different orders using only a single lens and detector to simultaneously detect both the magnitude and direction of translation and tilt by capturing only two frames: one before and another after the object motion. The dynamic range and sensitivity of the measurement can be varied readily by altering the position of the mirror/s used in the optical setup. Theoretical analysis and experiment results are presented.

Digital Correlation In Laser-Speckle Velocimetry

NASA Technical Reports Server (NTRS)

Gilbert, John A.; Mathys, Donald R.

1992-01-01

Periodic recording helps to eliminate spurious results. Improved digital-correlation process extracts velocity field of two-dimensional flow from laser-speckle images of seed particles distributed sparsely in flow. Method which involves digital correlation of images recorded at unequal intervals, completely automated and has potential to be fastest yet.

Tandem-pulsed acousto-optics: an analytical framework of modulated high-contrast speckle patterns.

PubMed

Resink, S G; Steenbergen, W

2015-06-07

Recently we presented acousto-optic (AO) probing of scattering media using addition or subtraction of speckle patterns due to tandem nanosecond pulses. Here we present a theoretical framework for ideal (polarized, noise-free) speckle patterns with unity contrast that links ultrasound-induced optical phase modulation, the fraction of light that is tagged by ultrasound, speckle contrast, mean square difference of speckle patterns and the contrast of the summation of speckle patterns acquired at different ultrasound phases. We derive the important relations from basic assumptions and definitions, and then validate them with simulations. For ultrasound-generated phase modulation angles below 0.7 rad (assuming uniform modulation), we are now able to relate speckle pattern statistics to the acousto-optic phase modulation. Hence our theory allows quantifying speckle observations in terms of ultrasonically tagged fractions of light for near-unity-contrast speckle patterns.

Visually lossless compression of digital hologram sequences

NASA Astrophysics Data System (ADS)

Darakis, Emmanouil; Kowiel, Marcin; Näsänen, Risto; Naughton, Thomas J.

2010-01-01

Digital hologram sequences have great potential for the recording of 3D scenes of moving macroscopic objects as their numerical reconstruction can yield a range of perspective views of the scene. Digital holograms inherently have large information content and lossless coding of holographic data is rather inefficient due to the speckled nature of the interference fringes they contain. Lossy coding of still holograms and hologram sequences has shown promising results. By definition, lossy compression introduces errors in the reconstruction. In all of the previous studies, numerical metrics were used to measure the compression error and through it, the coding quality. Digital hologram reconstructions are highly speckled and the speckle pattern is very sensitive to data changes. Hence, numerical quality metrics can be misleading. For example, for low compression ratios, a numerically significant coding error can have visually negligible effects. Yet, in several cases, it is of high interest to know how much lossy compression can be achieved, while maintaining the reconstruction quality at visually lossless levels. Using an experimental threshold estimation method, the staircase algorithm, we determined the highest compression ratio that was not perceptible to human observers for objects compressed with Dirac and MPEG-4 compression methods. This level of compression can be regarded as the point below which compression is perceptually lossless although physically the compression is lossy. It was found that up to 4 to 7.5 fold compression can be obtained with the above methods without any perceptible change in the appearance of video sequences.

Measurement of Strain Distributions in Mouse Femora with 3D-Digital Speckle Pattern Interferometry

PubMed Central

Yang, Lianxiang; Zhang, Ping; Liu, Sheng; Samala, Praveen R; Su, Min; Yokota, Hiroki

2007-01-01

Bone is a mechanosensitive tissue that adapts its mass, architecture and mechanical properties to external loading. Appropriate mechanical loads offer an effective means to stimulate bone remodeling and prevent bone loss. A role of in situ strain in bone is considered essential in enhancement of bone formation, and establishing a quantitative relationship between 3D strain distributions and a rate of local bone formation is important. Digital speckle pattern interferometry (DSPI) can achieve whole-field, non-contacting measurements of microscopic deformation for high-resolution determination of 3D strain distributions. However, the current system does not allow us to derive accurate strain distributions because of complex surface contours inherent to biological samples. Through development of a custom-made piezoelectric loading device as well as a new DSPI-based force calibration system, we built an advanced DSPI system and integrated local contour information to deformation data. Using a mouse femur in response to a knee loading modality as a model system, we determined 3D strain distributions and discussed effectiveness and limitations of the described system. PMID:18670581

Effect of signal intensity and camera quantization on laser speckle contrast analysis

PubMed Central

Song, Lipei; Elson, Daniel S.

2012-01-01

Laser speckle contrast analysis (LASCA) is limited to being a qualitative method for the measurement of blood flow and tissue perfusion as it is sensitive to the measurement configuration. The signal intensity is one of the parameters that can affect the contrast values due to the quantization of the signals by the camera and analog-to-digital converter (ADC). In this paper we deduce the theoretical relationship between signal intensity and contrast values based on the probability density function (PDF) of the speckle pattern and simplify it to a rational function. A simple method to correct this contrast error is suggested. The experimental results demonstrate that this relationship can effectively compensate the bias in contrast values induced by the quantized signal intensity and correct for bias induced by signal intensity variations across the field of view. PMID:23304650

Measurement of concentration of sugar in solutions with laser speckle decorrelation

NASA Astrophysics Data System (ADS)

Mahajan, Swapnil; Trivedi, Vismay; Chhaniwal, Vani; Prajapati, Mahendra; Zalevsky, Zeev; Javidi, Bahram; Anand, Arun

2015-05-01

Measurement of rotation of plane of polarization of linearly polarized light can provide information about the concentration of the optically active system with which it interacts. For substances containing sugar, accurate measurement of rotation of linearly polarized light can provide quantitative information about concentration of sugar in the material. Measurement of sugar concentration is important in areas ranging from blood sugar level measurement in body fluids to measurement of sugar concentrations in juices and other beverages. But in many of these cases, the changes introduced to the state of polarization considering a sample of practical proportion is low and the measurement of low optical rotations becomes necessary. So methods with higher sensitivity, accuracy and resolution need to be developed for the measurement of low optical rotations. Here we describe the development of a compact, low cost, field portable, device for rotation sensing leading to sugar concentration measurements, using speckle de-correlation technique. The developed device measures rotations by determining the changes occurring to a speckle pattern generated by a laser beam passing through the medium under investigation. The device consists of a sample chamber, a diode laser module, a ground glass diffuser and a digital sensor for recording of laser speckle patterns. The device was found to have high resolution and sensitivity.

Fluorescent speckle microscopy of microtubules: how low can you go?

PubMed

Waterman-Storer, C M; Salmon, E D

1999-12-01

Fluorescent speckle microscopy (FSM) is a new technique for visualizing the movement, assembly, and turnover of macromolecular assemblies like the cytoskeleton in living cells. In this method, contrast is created by coassembly of a small fraction of fluorescent subunits in a pool of unlabeled subunits. Random variation in association creates a nonuniform "fluorescent speckle" pattern. Fluorescent speckle movements in time-lapse recordings stand out to the eye and can be measured. Because fluorescent speckles represent fiduciary marks on the polymer lattice, FSM provides the opportunity for the first time to see the 2- and 3-dimensional trajectories of lattice movements within large arrays of polymers as well as identifying sites of assembly and disassembly of individual polymers. The technique works with either microinjection of fluorescently labeled subunits or expression of subunits ligated to green fluorescent protein (GFP). We have found for microtubules assembled in vitro that speckles containing one fluorophore can be detected and recorded using a conventional wide-field epi-fluorescence light microscope and digital imaging with a low noise cooled CCD camera. In living cells, optimal speckle contrast occurs at fractions of labeled tubulin of approximately 0.1-0.5% where the fluorescence of each speckle corresponds to one to seven fluorophores per resolvable unit (approximately 0.27 microm) in the microscope. This small fraction of labeled subunits significantly reduces out-of-focus fluorescence and greatly improves visibility of fluorescently labeled structures and their dynamics in thick regions of living cells.

Speckle noise reduction in digital holography by slightly rotating the object

NASA Astrophysics Data System (ADS)

Herrera-Ramirez, Jorge; Hincapie-Zuluaga, Diego Andrés; Garcia-Sucerquia, Jorge

2016-12-01

This work shows the realization of speckle reduction in the numerical reconstruction of digitally recorded holograms by the superposition of multiple slightly rotated digital holographic images of the object. The superposition of T uncorrelated holographic images reduces the contrast of the speckle noise of the image following the expected 1/√{T} law. The effect of the method on the borders of the resulting image is evaluated by quantifying the utilization of the dynamic range or the contrast between the white and black areas of a regular die. Experimental results validate the feasibility of the proposed method.

Detection of white spot lesions by segmenting laser speckle images using computer vision methods.

PubMed

Gavinho, Luciano G; Araujo, Sidnei A; Bussadori, Sandra K; Silva, João V P; Deana, Alessandro M

2018-05-05

This paper aims to develop a method for laser speckle image segmentation of tooth surfaces for diagnosis of early stages caries. The method, applied directly to a raw image obtained by digital photography, is based on the difference between the speckle pattern of a carious lesion tooth surface area and that of a sound area. Each image is divided into blocks which are identified in a working matrix by their χ 2 distance between block histograms of the analyzed image and the reference histograms previously obtained by K-means from healthy (h_Sound) and lesioned (h_Decay) areas, separately. If the χ 2 distance between a block histogram and h_Sound is greater than the distance to h_Decay, this block is marked as decayed. The experiments showed that the method can provide effective segmentation for initial lesions. We used 64 images to test the algorithm and we achieved 100% accuracy in segmentation. Differences between the speckle pattern of a sound tooth surface region and a carious region, even in the early stage, can be evidenced by the χ 2 distance between histograms. This method proves to be more effective for segmenting the laser speckle image, which enhances the contrast between sound and lesioned tissues. The results were obtained with low computational cost. The method has the potential for early diagnosis in a clinical environment, through the development of low-cost portable equipment.

Optimisation and Validation of the ARAMIS Digital Image Correlation System for Use in Large-scale High-strain-rate Events

DTIC Science & Technology

2013-08-01

enamel paint. Under extreme plastic deformation, the relative deformation of the coating could cause the coating to separate resulting in loss of...point for one to be found. If a discontinuity, such as a crack , occurs through the object separating speckle pattern, then the strain data will only

Advanced image based methods for structural integrity monitoring: Review and prospects

NASA Astrophysics Data System (ADS)

Farahani, Behzad V.; Sousa, Pedro José; Barros, Francisco; Tavares, Paulo J.; Moreira, Pedro M. G. P.

2018-02-01

There is a growing trend in engineering to develop methods for structural integrity monitoring and characterization of in-service mechanical behaviour of components. The fast growth in recent years of image processing techniques and image-based sensing for experimental mechanics, brought about a paradigm change in phenomena sensing. Hence, several widely applicable optical approaches are playing a significant role in support of experiment. The current review manuscript describes advanced image based methods for structural integrity monitoring, and focuses on methods such as Digital Image Correlation (DIC), Thermoelastic Stress Analysis (TSA), Electronic Speckle Pattern Interferometry (ESPI) and Speckle Pattern Shearing Interferometry (Shearography). These non-contact full-field techniques rely on intensive image processing methods to measure mechanical behaviour, and evolve even as reviews such as this are being written, which justifies a special effort to keep abreast of this progress.

Laser Speckle Imaging of Cerebral Blood Flow

NASA Astrophysics Data System (ADS)

Luo, Qingming; Jiang, Chao; Li, Pengcheng; Cheng, Haiying; Wang, Zhen; Wang, Zheng; Tuchin, Valery V.

Monitoring the spatio-temporal characteristics of cerebral blood flow (CBF) is crucial for studying the normal and pathophysiologic conditions of brain metabolism. By illuminating the cortex with laser light and imaging the resulting speckle pattern, relative CBF images with tens of microns spatial and millisecond temporal resolution can be obtained. In this chapter, a laser speckle imaging (LSI) method for monitoring dynamic, high-resolution CBF is introduced. To improve the spatial resolution of current LSI, a modified LSI method is proposed. To accelerate the speed of data processing, three LSI data processing frameworks based on graphics processing unit (GPU), digital signal processor (DSP), and field-programmable gate array (FPGA) are also presented. Applications for detecting the changes in local CBF induced by sensory stimulation and thermal stimulation, the influence of a chemical agent on CBF, and the influence of acute hyperglycemia following cortical spreading depression on CBF are given.

Speckle reduction in digital holography with resampling ring masks

NASA Astrophysics Data System (ADS)

Zhang, Wenhui; Cao, Liangcai; Jin, Guofan

2018-01-01

One-shot digital holographic imaging has the advantages of high stability and low temporal cost. However, the reconstruction is affected by the speckle noise. Resampling ring-mask method in spectrum domain is proposed for speckle reduction. The useful spectrum of one hologram is divided into several sub-spectra by ring masks. In the reconstruction, angular spectrum transform is applied to guarantee the calculation accuracy which has no approximation. N reconstructed amplitude images are calculated from the corresponding sub-spectra. Thanks to speckle's random distribution, superimposing these N uncorrelated amplitude images would lead to a final reconstructed image with lower speckle noise. Normalized relative standard deviation values of the reconstructed image are used to evaluate the reduction of speckle. Effect of the method on the spatial resolution of the reconstructed image is also quantitatively evaluated. Experimental and simulation results prove the feasibility and effectiveness of the proposed method.

Lensless digital holography with diffuse illumination through a pseudo-random phase mask.

PubMed

Bernet, Stefan; Harm, Walter; Jesacher, Alexander; Ritsch-Marte, Monika

2011-12-05

Microscopic imaging with a setup consisting of a pseudo-random phase mask, and an open CMOS camera, without an imaging objective, is demonstrated. The pseudo random phase mask acts as a diffuser for an incoming laser beam, scattering a speckle pattern to a CMOS chip, which is recorded once as a reference. A sample which is afterwards inserted somewhere in the optical beam path changes the speckle pattern. A single (non-iterative) image processing step, comparing the modified speckle pattern with the previously recorded one, generates a sharp image of the sample. After a first calibration the method works in real-time and allows quantitative imaging of complex (amplitude and phase) samples in an extended three-dimensional volume. Since no lenses are used, the method is free from lens abberations. Compared to standard inline holography the diffuse sample illumination improves the axial sectioning capability by increasing the effective numerical aperture in the illumination path, and it suppresses the undesired so-called twin images. For demonstration, a high resolution spatial light modulator (SLM) is programmed to act as the pseudo-random phase mask. We show experimental results, imaging microscopic biological samples, e.g. insects, within an extended volume at a distance of 15 cm with a transverse and longitudinal resolution of about 60 μm and 400 μm, respectively.

Simple Educational Tool for Digital Speckle Shearography

ERIC Educational Resources Information Center

Spagnolo, Giuseppe Schirripa; Martocchia, Andrea; Papalillo, Donato; Cozzella, Lorenzo

2012-01-01

In this study, an educational tool has been prepared for obtaining short-term and more economic training on digital speckle shearography (DSS). Shearography non-destructive testing (NDT) has gained wide acceptance over the last decade, providing a number of important and exciting inspection solutions in aerospace, electronics and medical device…

Digital Processing Of Young's Fringes In Speckle Photography

NASA Astrophysics Data System (ADS)

Chen, D. J.; Chiang, F. P.

1989-01-01

A new technique for fully automatic diffraction fringe measurement in point-wise speckle photograph analysis is presented in this paper. The fringe orientation and spacing are initially estimated with the help of 1-D FFT. A 2-D convolution filter is then applied to enhance the estimated image . High signal-to-noise rate (SNR) fringe pattern is achieved which makes it feasible for precise determination of the displacement components. The halo-effect is also optimally eliminated in a new way. With the computation time compared favorably with those of 2-D autocorrelation method and the iterative 2-D FFT method. High reliability and accurate determination of displacement components are achieved over a wide range of fringe density.

Application of Optical Measurement Techniques During Stages of Pregnancy: Use of Phantom High Speed Cameras for Digital Image Correlation (D.I.C.) During Baby Kicking and Abdomen Movements

NASA Technical Reports Server (NTRS)

Gradl, Paul

2016-01-01

Paired images were collected using a projected pattern instead of standard painting of the speckle pattern on her abdomen. High Speed cameras were post triggered after movements felt. Data was collected at 120 fps -limited due to 60hz frequency of projector. To ensure that kicks and movement data was real a background test was conducted with no baby movement (to correct for breathing and body motion).

Laser speckle based digital optical methods in structural mechanics: A review

NASA Astrophysics Data System (ADS)

De la Torre, I. Manuel; Hernández Montes, María del Socorro; Flores-Moreno, J. Mauricio; Santoyo, Fernando Mendoza

2016-12-01

Laser Speckle Correlation, Electronic Speckle Pattern Interferometry and Digital Holographic interferometry have evolved for decades to become relevant techniques in many fields of today's wide spectrum of knowledge and disciplines. Indeed, with today's advances in optics, photonics, electronics and computing there are many important applications for them and strictly speaking there are an almost infinite number of applications that one can think of, as they are non-contact optical techniques that can be used to measure mechanical parameters ranging from a few microns to hundreds of nanometers. In this review we will explore and discuss some relevant applications in structural mechanics in the fields of materials in engineering, biomedical and art preservation and restoration. This work will take the reader from a succinct historical account on the development of these techniques, followed by a brief theoretical description for each one that will then facilitate the introduction of the results chosen as the key applications, ending the review with the conclusions. From the myriad of papers now available in the web, we will only present those that we believe are the most illustrative applications within three lustrum, 2000 to 2015, all set to give a frame that place these optical techniques as mature technologies with an absolute relevance to conduct metrology in many fields.

Progress in high temperature speckle-shift strain measurement system

NASA Technical Reports Server (NTRS)

Lant, Christian T.; Barranger, John P.

1990-01-01

A fast, easy to use speckle tracking system is under development for the speckle-shift strain measurement technique. Preliminary correlation tests on wire specimens show strong correlations of well-developed speckle patterns. Stable cross-correlations were obtained from a tungsten filament at 2480 C. An analysis of the optical system determines the minimum required sampling frequency of the speckle pattern to be 2.55 pixels per speckle.

Real-time optical fiber digital speckle pattern interferometry for industrial applications

NASA Astrophysics Data System (ADS)

Chan, Robert K.; Cheung, Y. M.; Lo, C. H.; Tam, T. K.

1997-03-01

There is current interest, especially in the industrial sector, to use the digital speckle pattern interferometry (DSPI) technique to measure surface stress. Indeed, many publications in the subject are evident of the growing interests in the field. However, to bring the technology to industrial use requires the integration of several emerging technologies, viz. optics, feedback control, electronics, imaging processing and digital signal processing. Due to the highly interdisciplinary nature of the technique, successful implementation and development require expertise in all of the fields. At Baptist University, under the funding of a major industrial grant, we are developing the technology for the industrial sector. Our system fully exploits optical fibers and diode lasers in the design to enable practical and rugged systems suited for industrial applications. Besides the development in optics, we have broken away from the reliance of a microcomputer PC platform for both image capture and processing, and have developed a digital signal processing array system that can handle simultaneous and independent image capture/processing with feedback control. The system, named CASPA for 'cascadable architecture signal processing array,' is a third generation development system that utilizes up to 7 digital signal processors has proved to be a very powerful system. With our CASPA we are now in a better position to developing novel optical measurement systems for industrial application that may require different measurement systems to operate concurrently and requiring information exchange between the systems. Applications in mind such as simultaneous in-plane and out-of-plane DSPI image capture/process, vibrational analysis with interactive DSPI and phase shifting control of optical systems are a few good examples of the potentials.

Automated analysis of art object surfaces using time-averaged digital speckle pattern interferometry

NASA Astrophysics Data System (ADS)

Lukomski, Michal; Krzemien, Leszek

2013-05-01

Technical development and practical evaluation of a laboratory built, out-of-plane digital speckle pattern interferometer (DSPI) are reported. The instrument was used for non-invasive, non-contact detection and characterization of early-stage damage, like fracturing and layer separation, of painted objects of art. A fully automated algorithm was developed for recording and analysis of vibrating objects utilizing continuous-wave laser light. The algorithm uses direct, numerical fitting or Hilbert transformation for an independent, quantitative evaluation of the Bessel function at every point of the investigated surface. The procedure does not require phase modulation and thus can be implemented within any, even the simplest, DSPI apparatus. The proposed deformation analysis is fast and computationally inexpensive. Diagnosis of physical state of the surface of a panel painting attributed to Nicolaus Haberschrack (a late-mediaeval painter active in Krakow) from the collection of the National Museum in Krakow is presented as an example of an in situ application of the developed methodology. It has allowed the effectiveness of the deformation analysis to be evaluated for the surface of a real painting (heterogeneous colour and texture) in a conservation studio where vibration level was considerably higher than in the laboratory. It has been established that the methodology, which offers automatic analysis of the interferometric fringe patterns, has a considerable potential to facilitate and render more precise the condition surveys of works of art.

High-contrast multilayer imaging of biological organisms through dark-field digital refocusing.

PubMed

Faridian, Ahmad; Pedrini, Giancarlo; Osten, Wolfgang

2013-08-01

We have developed an imaging system to extract high contrast images from different layers of biological organisms. Utilizing a digital holographic approach, the system works without scanning through layers of the specimen. In dark-field illumination, scattered light has the main contribution in image formation, but in the case of coherent illumination, this creates a strong speckle noise that reduces the image quality. To remove this restriction, the specimen has been illuminated with various speckle-fields and a hologram has been recorded for each speckle-field. Each hologram has been analyzed separately and the corresponding intensity image has been reconstructed. The final image has been derived by averaging over the reconstructed images. A correlation approach has been utilized to determine the number of speckle-fields required to achieve a desired contrast and image quality. The reconstructed intensity images in different object layers are shown for different sea urchin larvae. Two multimedia files are attached to illustrate the process of digital focusing.

Speckle-interferometric measurement system of 3D deformation to obtain thickness changes of thin specimen under tensile loads

NASA Astrophysics Data System (ADS)

Kowarsch, Robert; Zhang, Jiajun; Sguazzo, Carmen; Hartmann, Stefan; Rembe, Christian

2017-06-01

The analysis of materials and geometries in tensile tests and the extraction of mechanic parameters is an important field in solid mechanics. Especially the measurement of thickness changes is important to obtain accurate strain information of specimens under tensile loads. Current optical measurement methods comprising 3D digital image correlation enable thickness-change measurement only with nm-resolution. We present a phase-shifting electronic speckle-pattern interferometer in combination with speckle-correlation technique to measure the 3D deformation. The phase-shift for the interferometer is introduced by fast wavelength tuning of a visible diode laser by injection current. In a post-processing step, both measurements can be combined to reconstruct the 3D deformation. In this contribution, results of a 3Ddeformation measurement for a polymer membrane are presented. These measurements show sufficient resolution for the detection of 3D deformations of thin specimen in tensile test. In future work we address the thickness changes of thin specimen under tensile loads.

Optimization of algorithm of coding of genetic information of Chlamydia

NASA Astrophysics Data System (ADS)

Feodorova, Valentina A.; Ulyanov, Sergey S.; Zaytsev, Sergey S.; Saltykov, Yury V.; Ulianova, Onega V.

2018-04-01

New method of coding of genetic information using coherent optical fields is developed. Universal technique of transformation of nucleotide sequences of bacterial gene into laser speckle pattern is suggested. Reference speckle patterns of the nucleotide sequences of omp1 gene of typical wild strains of Chlamydia trachomatis of genovars D, E, F, G, J and K and Chlamydia psittaci serovar I as well are generated. Algorithm of coding of gene information into speckle pattern is optimized. Fully developed speckles with Gaussian statistics for gene-based speckles have been used as criterion of optimization.

Suppression of Speckles at High Adaptive Correction Using Speckle Symmetry

NASA Technical Reports Server (NTRS)

Bloemhof, Eric E.

2006-01-01

Focal-plane speckles set important sensitivity limits on ground- or space-based imagers and coronagraphs that may be used to search for faint companions, perhaps ultimately including exoplanets, around stars. As speckles vary with atmospheric fluctuations or with drifting beamtrain aberrations, they contribute speckle noise proportional to their full amplitude. Schemes to suppress speckles are thus of great interest. At high adaptive correction, speckles organize into species, represented by algebraic terms in the expansion of the phase exponential, that have distinct spatial symmetry, even or odd, under spatial inversion. Filtering speckle patterns by symmetry may eliminate a disproportionate fraction of the speckle noise while blocking (only) half of the image signal from the off-axis companion being sought. The fraction of speckle power and hence of speckle noise in each term will vary with degree of correction, and so also will the net symmetry in the speckle pattern.

Digital micromirror device as programmable rough particle in interferometric particle imaging.

PubMed

Fromager, M; Aït Ameur, K; Brunel, M

2017-04-20

The 2D autocorrelation of the projection of an irregular rough particle can be estimated using the analysis of its interferometric out-of-focus image. We report the development of an experimental setup that creates speckle-like patterns generated by "programmable" rough particles of desired-shape. It should become an important tool for the development of new setups, configurations, and algorithms in interferometric particle imaging.

Correlated tuning of the speckle pattern in an interferometer based on a multimode fiber-optic waveguide

NASA Astrophysics Data System (ADS)

Bykovskii, Iu. A.; Kul'Chin, Iu. N.; Obukh, V. F.; Smirnov, V. L.

1990-08-01

The correlated tuning of the speckle pattern in the radiation field of a single-fiber multimode interferometer is investigated experimentally and analytically in the presence of external action. It is found that correlated changes in the speckle pattern are observed in both the near and the far emission fields of the waveguide. An expression is obtained which provides a way to determine the maximum size of the speckle correlation region. The use of spatial filtering for isolating the effect of correlated speckle pattern tuning is suggested. It is shown that the use of a spatial filter makes it possible to increase the efficiency of fiber-optic transducers.

Atherosclerotic plaque characterization by spatial and temporal speckle pattern analysis

NASA Astrophysics Data System (ADS)

Tearney, Guillermo J.; Bouma, Brett E.

2002-04-01

Improved methods are needed to identify the vulnerable coronary plaques responsible for acute myocardial infraction or sudden cardiac death. We describe a method for characterizing the structure and biomechanical properties of atherosclerotic plaques based on speckle pattern fluctuations. Near-field speckle images were acquired from five human aortic specimens ex vivo. The speckle decorrelation time constant varied significantly for vulnerable aortic plaques (τ = 40 ms) versus stable plaques (τ = 400 ms) and normal aorta (τ = 500 ms). These initial results indicate that different atherosclerotic plaque types may be distinguished by analysis of temporal and spatial speckle pattern fluctuations.

In vivo burn diagnosis by camera-phone diffuse reflectance laser speckle detection.

PubMed

Ragol, S; Remer, I; Shoham, Y; Hazan, S; Willenz, U; Sinelnikov, I; Dronov, V; Rosenberg, L; Bilenca, A

2016-01-01

Burn diagnosis using laser speckle light typically employs widefield illumination of the burn region to produce two-dimensional speckle patterns from light backscattered from the entire irradiated tissue volume. Analysis of speckle contrast in these time-integrated patterns can then provide information on burn severity. Here, by contrast, we use point illumination to generate diffuse reflectance laser speckle patterns of the burn. By examining spatiotemporal fluctuations in these time-integrated patterns along the radial direction from the incident point beam, we show the ability to distinguish partial-thickness burns in a porcine model in vivo within the first 24 hours post-burn. Furthermore, our findings suggest that time-integrated diffuse reflectance laser speckle can be useful for monitoring burn healing over time post-burn. Unlike conventional diffuse reflectance laser speckle detection systems that utilize scientific or industrial-grade cameras, our system is designed with a camera-phone, demonstrating the potential for burn diagnosis with a simple imager.

In vivo burn diagnosis by camera-phone diffuse reflectance laser speckle detection

PubMed Central

Ragol, S.; Remer, I.; Shoham, Y.; Hazan, S.; Willenz, U.; Sinelnikov, I.; Dronov, V.; Rosenberg, L.; Bilenca, A.

2015-01-01

Burn diagnosis using laser speckle light typically employs widefield illumination of the burn region to produce two-dimensional speckle patterns from light backscattered from the entire irradiated tissue volume. Analysis of speckle contrast in these time-integrated patterns can then provide information on burn severity. Here, by contrast, we use point illumination to generate diffuse reflectance laser speckle patterns of the burn. By examining spatiotemporal fluctuations in these time-integrated patterns along the radial direction from the incident point beam, we show the ability to distinguish partial-thickness burns in a porcine model in vivo within the first 24 hours post-burn. Furthermore, our findings suggest that time-integrated diffuse reflectance laser speckle can be useful for monitoring burn healing over time post-burn. Unlike conventional diffuse reflectance laser speckle detection systems that utilize scientific or industrial-grade cameras, our system is designed with a camera-phone, demonstrating the potential for burn diagnosis with a simple imager. PMID:26819831

Noninvasive, three-dimensional full-field body sensor for surface deformation monitoring of human body in vivo

NASA Astrophysics Data System (ADS)

Chen, Zhenning; Shao, Xinxing; He, Xiaoyuan; Wu, Jialin; Xu, Xiangyang; Zhang, Jinlin

2017-09-01

Noninvasive, three-dimensional (3-D), full-field surface deformation measurements of the human body are important for biomedical investigations. We proposed a 3-D noninvasive, full-field body sensor based on stereo digital image correlation (stereo-DIC) for surface deformation monitoring of the human body in vivo. First, by applying an improved water-transfer printing (WTP) technique to transfer optimized speckle patterns onto the skin, the body sensor was conveniently and harmlessly fabricated directly onto the human body. Then, stereo-DIC was used to achieve 3-D noncontact and noninvasive surface deformation measurements. The accuracy and efficiency of the proposed body sensor were verified and discussed by considering different complexions. Moreover, the fabrication of speckle patterns on human skin, which has always been considered a challenging problem, was shown to be feasible, effective, and harmless as a result of the improved WTP technique. An application of the proposed stereo-DIC-based body sensor was demonstrated by measuring the pulse wave velocity of human carotid artery.

Adaptive correction to the speckle correlation fringes by using a twisted-nematic liquid-crystal display.

PubMed

Hack, Erwin; Gundu, Phanindra Narayan; Rastogi, Pramod

2005-05-10

An innovative technique for reducing speckle noise and improving the intensity profile of the speckle correlation fringes is presented. The method is based on reducing the range of the modulation intensity values of the speckle interference pattern. After the fringe pattern is corrected adaptively at each pixel, a simple morphological filtering of the fringes is sufficient to obtain smoothed fringes. The concept is presented both analytically and by simulation by using computer-generated speckle patterns. The experimental verification is performed by using an amplitude-only spatial light modulator (SLM) in a conventional electronic speckle pattern interferometry setup. The optical arrangement for tuning a commercially available LCD array for amplitude-only behavior is described. The method of feedback to the LCD SLM to modulate the intensity of the reference beam in order to reduce the modulation intensity values is explained, and the resulting fringe pattern and increase in the signal-to-noise ratio are discussed.

Measurement of strain distribution in cortical bone around miniscrew implants used for orthodontic anchorage using digital speckle pattern interferometry

NASA Astrophysics Data System (ADS)

Kumar, Manoj; Agarwal, Rupali; Bhutani, Ravi; Shakher, Chandra

2016-05-01

An application of digital speckle pattern interferometry (DSPI) for the measurement of deformations and strain-field distributions developed in cortical bone around orthodontic miniscrew implants inserted into the human maxilla is presented. The purpose of this study is to measure and compare the strain distribution in cortical bone/miniscrew interface of human maxilla around miniscrew implants of different diameters, different implant lengths, and implants of different commercially available companies. The technique is also used to measure tilt/rotation of canine caused due to the application of retraction springs. The proposed technique has high sensitivity and enables the observation of deformation/strain distribution. In DSPI, two specklegrams are recorded corresponding to pre- and postloading of the retraction spring. The DSPI fringe pattern is observed by subtracting these two specklegrams. Optical phase was extracted using Riesz transform and the monogenic signal from a single DSPI fringe pattern. The obtained phase is used to calculate the parameters of interest such as displacement/deformation and strain/stress. The experiment was conducted on a dry human skull fulfilling the criteria of intact dental arches and all teeth present. Eight different miniscrew implants were loaded with an insertion angulation of 45 deg in the inter-radicular region of the maxillary second premolar and molar region. The loading of miniscrew implants was done with force level (150 gf) by nickel-titanium closed-coil springs (9 mm). The obtained results from DSPI reveal that implant diameter and implant length affect the displacement and strain distribution in cortical bone layer surrounding the miniscrew implant.

Speckle reduction in laser projection using a dynamic deformable mirror.

PubMed

Tran, Thi-Kim-Trinh; Chen, Xuyuan; Svensen, Øyvind; Akram, Muhammad Nadeem

2014-05-05

Despite of much effort and significant progress in recent years, speckle removal is still a challenge for laser projection technology. In this paper, speckle reduction by dynamic deformable mirror was investigated. Time varying independent speckle patterns were generated due to the angle diversity introduced by the dynamic mirror, and these speckle patterns were averaged out by the camera or human eyes, thus reducing speckle contrast in the final image. The speckle reduction by the wavelength diversity of the lasers was also studied. Both broadband lasers and narrowband laser were used for experiment. It is experimentally shown that speckle suppression can be attained by the widening of the spectrum of the lasers. Lower speckle contrast reduction was attained by the wavelength diversity for narrowband laser compared to the broadband lasers. This method of speckle reduction is suitable in laser projectors for wide screen applications where high power laser illumination is needed.

Interference in astronomical speckle patterns

NASA Technical Reports Server (NTRS)

Breckinridge, J. B.

1976-01-01

Astronomical speckle patterns are examined in an atmospheric-optics context in order to determine what kind of image quality is to be expected from several different imaging techniques. The model used to describe the instantaneous complex field distribution across the pupil of a large telescope regards the pupil as a deep phase grating with a periodicity given by the size of the cell of uniform phase or the refractive index structure function. This model is used along with an empirical formula derived purely from the physical appearance of the speckle patterns to discuss the orders of interference in astronomical speckle patterns.

Accurate reconstruction in digital holographic microscopy using antialiasing shift-invariant contourlet transform

NASA Astrophysics Data System (ADS)

Zhang, Xiaolei; Zhang, Xiangchao; Xu, Min; Zhang, Hao; Jiang, Xiangqian

2018-03-01

The measurement of microstructured components is a challenging task in optical engineering. Digital holographic microscopy has attracted intensive attention due to its remarkable capability of measuring complex surfaces. However, speckles arise in the recorded interferometric holograms, and they will degrade the reconstructed wavefronts. Existing speckle removal methods suffer from the problems of frequency aliasing and phase distortions. A reconstruction method based on the antialiasing shift-invariant contourlet transform (ASCT) is developed. Salient edges and corners have sparse representations in the transform domain of ASCT, and speckles can be recognized and removed effectively. As subsampling in the scale and directional filtering schemes is avoided, the problems of frequency aliasing and phase distortions occurring in the conventional multiscale transforms can be effectively overcome, thereby improving the accuracy of wavefront reconstruction. As a result, the proposed method is promising for the digital holographic measurement of complex structures.

Shear wave arrival time estimates correlate with local speckle pattern.

PubMed

Mcaleavey, Stephen A; Osapoetra, Laurentius O; Langdon, Jonathan

2015-12-01

We present simulation and phantom studies demonstrating a strong correlation between errors in shear wave arrival time estimates and the lateral position of the local speckle pattern in targets with fully developed speckle. We hypothesize that the observed arrival time variations are largely due to the underlying speckle pattern, and call the effect speckle bias. Arrival time estimation is a key step in quantitative shear wave elastography, performed by tracking tissue motion via cross-correlation of RF ultrasound echoes or similar methods. Variations in scatterer strength and interference of echoes from scatterers within the tracking beam result in an echo that does not necessarily describe the average motion within the beam, but one favoring areas of constructive interference and strong scattering. A swept-receive image, formed by fixing the transmit beam and sweeping the receive aperture over the region of interest, is used to estimate the local speckle pattern. Metrics for the lateral position of the speckle are found to correlate strongly (r > 0.7) with the estimated shear wave arrival times both in simulations and in phantoms. Lateral weighting of the swept-receive pattern improved the correlation between arrival time estimates and speckle position. The simulations indicate that high RF echo correlation does not equate to an accurate shear wave arrival time estimate-a high correlation coefficient indicates that motion is being tracked with high precision, but the location tracked is uncertain within the tracking beam width. The presence of a strong on-axis speckle is seen to imply high RF correlation and low bias. The converse does not appear to be true-highly correlated RF echoes can still produce biased arrival time estimates. The shear wave arrival time bias is relatively stable with variations in shear wave amplitude and sign (-20 μm to 20 μm simulated) compared with the variation with different speckle realizations obtained along a given tracking vector. We show that the arrival time bias is weakly dependent on shear wave amplitude compared with the variation with axial position/ local speckle pattern. Apertures of f/3 to f/8 on transmit and f/2 and f/4 on receive were simulated. Arrival time error and correlation with speckle pattern are most strongly determined by the receive aperture.

Shear Wave Arrival Time Estimates Correlate with Local Speckle Pattern

PubMed Central

McAleavey, Stephen A.; Osapoetra, Laurentius O.; Langdon, Jonathan

2016-01-01

We present simulation and phantom studies demonstrating a strong correlation between errors in shear wave arrival time estimates and the lateral position of the local speckle pattern in targets with fully developed speckle. We hypothesize that the observed arrival time variations are largely due to the underlying speckle pattern, and call the effect speckle bias. Arrival time estimation is a key step in quantitative shear wave elastography, performed by tracking tissue motion via cross correlation of RF ultrasound echoes or similar methods. Variations in scatterer strength and interference of echoes from scatterers within the tracking beam result in an echo that does not necessarily describe the average motion within the beam, but one favoring areas of constructive interference and strong scattering. A swept-receive image, formed by fixing the transmit beam and sweeping the receive aperture over the region of interest, is used to estimate the local speckle pattern. Metrics for the lateral position of the speckle are found to correlate strongly (r>0.7) with the estimated shear wave arrival times both in simulations and in phantoms. Lateral weighting of the swept-receive pattern improved the correlation between arrival time estimates and speckle position. The simulations indicate that high RF echo correlation does not equate to an accurate shear wave arrival time estimate – a high correlation coefficient indicates that motion is being tracked with high precision, but the location tracked is uncertain within the tracking beam width. The presence of a strong on-axis speckle is seen to imply high RF correlation and low bias. The converse does not appear to be true – highly correlated RF echoes can still produce biased arrival time estimates. The shear wave arrival time bias is relatively stable with variations in shear wave amplitude and sign (−20 μm to 20 μm simulated) compared to the variation with different speckle realizations obtained along a given tracking vector. We show that the arrival time bias is weakly dependent on shear wave amplitude compared to the variation with axial position/local speckle pattern. Apertures of f/3 to f/8 on transmit and f/2 and f/4 on receive were simulated. Arrival time error and correlation with speckle pattern are most strongly determined by the receive aperture. PMID:26670847

Visual based laser speckle pattern recognition method for structural health monitoring

NASA Astrophysics Data System (ADS)

Park, Kyeongtaek; Torbol, Marco

2017-04-01

This study performed the system identification of a target structure by analyzing the laser speckle pattern taken by a camera. The laser speckle pattern is generated by the diffuse reflection of the laser beam on a rough surface of the target structure. The camera, equipped with a red filter, records the scattered speckle particles of the laser light in real time and the raw speckle image of the pixel data is fed to the graphic processing unit (GPU) in the system. The algorithm for laser speckle contrast analysis (LASCA) computes: the laser speckle contrast images and the laser speckle flow images. The k-mean clustering algorithm is used to classify the pixels in each frame and the clusters' centroids, which function as virtual sensors, track the displacement between different frames in time domain. The fast Fourier transform (FFT) and the frequency domain decomposition (FDD) compute the modal properties of the structure: natural frequencies and damping ratios. This study takes advantage of the large scale computational capability of GPU. The algorithm is written in Compute Unifies Device Architecture (CUDA C) that allows the processing of speckle images in real time.

Numerical study on statistical properties of speckle pattern in laser projection display based on human eye model

NASA Astrophysics Data System (ADS)

Cui, Zhe; Wang, Anting; Ma, Qianli; Ming, Hai

2013-12-01

In this paper, the laser speckle pattern on human retina for a laser projection display is simulated. By introducing a specific eye model `Indiana Eye', the statistical properties of the laser speckle are numerical investigated. The results show that the aberrations of human eye (mostly spherical and chromatic) will decrease the speckle contrast felt by people. When the wavelength of the laser source is 550 nm (green), people will feel the strongest speck pattern and the weakest when the wavelength is 450 nm (blue). Myopia and hyperopia will decrease the speckle contrast by introducing large spherical aberrations. Although aberration is good for speckle reduction, but it will degrade the imaging capability of the eye. The results show that laser source (650 nm) will have the best image quality on the retina. At last, we compare the human eye with an aberration-free imaging system. Both the speckle contrast and the image quality appear different behavior in these two imaging systems. The results are useful when a standardized measurement procedure for speckle contrast needs to be built.

Speckle POF sensor for detecting vital signs of patients

NASA Astrophysics Data System (ADS)

Lomer, M.; Rodriguez-Cobo, L.; Revilla, P.; Herrero, G.; Madruga, F.; Lopez-Higuera, J. M.

2014-05-01

In this work, both arterial pulse and respiratory rate have been successfully measured based on changes in speckle patterns of multimode fibers. Using two fiber-based transducers, one located on the wrist and another in the chest, both disturbances were transmitted to the fiber, varying the speckle pattern. These variations of the speckle pattern were captured using a commercial webcam and further processed using different methods. The achieved results have been presented and the simultaneous monitoring of both vital signs has been also discussed. The feasibility to use the proposed sensor system for this application is demonstrated.

A Computer Based Moire Technique To Measure Very Small Displacements

NASA Astrophysics Data System (ADS)

Sciammarella, Cesar A.; Amadshahi, Mansour A.; Subbaraman, B.

1987-02-01

The accuracy that can be achieved in the measurement of very small displacements in techniques such as moire, holography and speckle is limited by the noise inherent to the utilized optical devices. To reduce the noise to signal ratio, the moire method can be utilized. Two system of carrier fringes are introduced, an initial system before the load is applied and a final system when the load is applied. The moire pattern of these two systems contains the sought displacement information and the noise common to the two patterns is eliminated. The whole process is performed by a computer on digitized versions of the patterns. Examples of application are given.

Vibration measurement of the tympanic membrane of guinea pig temporal bones using time-averaged speckle pattern interferometry

NASA Astrophysics Data System (ADS)

Wada, Hiroshi; Ando, Masayoshi; Takeuchi, Masataka; Sugawara, Hironori; Koike, Takuji; Kobayashi, Toshimitsu; Hozawa, Koji; Gemma, Takashi; Nara, Makoto

2002-05-01

``Time-averaged holography'' and ``holographic interferometry'' enable recording of the complete vibration pattern of a surface within several seconds. The results appear in the form of fringes. Vibration amplitudes smaller than 100 nm are not readily measurable by these techniques, because such small amplitudes produce variations in gray level, but not fringes. In practice, to obtain clear fringes in these measurements, stimulus sound pressures higher than 100 dB SPL must be used. The phase of motion is also not obtainable from such fringe techniques. In this study, a sinusoidal phase modulation technique is described, which allows detection of both small amplitudes of motion and their phase from time-averaged speckle pattern interferometry. In this technique, the laser injection current is modulated and digital image processing is used to analyze the measured patterns. When the sound-pressure level of stimuli is between 70 and 85 dB SPL, this system is applied to measure the vibratory response of the tympanic membrane (TM) of guinea pig temporal bones at frequencies up to 4 kHz where complicated vibration modes are observed. The effect of the bulla on TM displacements is also quantified. Results indicate that this system is capable of measuring the nanometer displacements of the TM, produced by stimuli of 70 dB SPL.

Changes in speckle patterns induced by load application onto an optical fiber and its possible application for sensing purpose

NASA Astrophysics Data System (ADS)

Hasegawa, Makoto; Okumura, Jyun-ya; Hyuga, Akio

2015-08-01

Speckle patterns to be observed in an output light spot from an optical fiber are known to be changed due to external disturbances applied onto the optical fiber. In order to investigate possibilities of utilizing such changes in speckle patterns for sensing application, a certain load was applied onto a jacket-covered communication-grade multi-mode glass optical fiber through which laser beams emitted from a laser diode were propagating, and observed changes in speckle patterns in the output light spot from the optical fiber were investigated both as image data via a CCD camera and as an output voltage from a photovoltaic panel irradiated with the output light spot. The load was applied via a load application mechanism in which several ridges were provided onto opposite flat plates and a certain number of weights were placed there so that corrugated bending of the optical fiber was intentionally induced via load application due to the ridges. The obtained results showed that the number of speckles in the observed pattern in the output light spot as well as the output voltage from the photovoltaic panel irradiated with the output light spot showed decreases upon load application with relatively satisfactory repeatability. When the load was reduced, i.e., the weights were removed, the number of speckles then showed recovery. These results indicate there is a certain possibility of utilizing changes in speckle patterns for sensing of load application onto the optical fiber.

Multiple speckle illumination for optical-resolution photoacoustic imaging

NASA Astrophysics Data System (ADS)

Poisson, Florian; Stasio, Nicolino; Moser, Christophe; Psaltis, Demetri; Bossy, Emmanuel

2017-03-01

Optical-resolution photoacoustic microscopy offers exquisite and specific contrast to optical absorption. Conventional approaches generally involves raster scanning a focused spot over the sample. Here, we demonstrate that a full-field illumination approach with multiple speckle illumination can also provide diffraction-limited optical-resolution photoacoustic images. Two different proof-of-concepts are demonstrated with micro-structured test samples. The first approach follows the principle of correlation/ghost imaging,1, 2 and is based on cross-correlating photoacoustic signals under multiple speckle illumination with known speckle patterns measured during a calibration step. The second approach is a speckle scanning microscopy technique, which adapts the technique proposed in fluorescence microscopy by Bertolotti and al.:3 in our work, spatially unresolved photoacoustic measurements are performed for various translations of unknown speckle patterns. A phase-retrieval algorithm is used to reconstruct the object from the knowledge of the modulus of its Fourier Transform yielded by the measurements. Because speckle patterns naturally appear in many various situations, including propagation through biological tissue or multi-mode fibers (for which focusing light is either very demanding if not impossible), speckle-illumination-based photoacoustic microscopy provides a powerful framework for the development of novel reconstruction approaches, well-suited to compressed sensing approaches.2

Super-resolution photoacoustic microscopy using joint sparsity

NASA Astrophysics Data System (ADS)

Burgholzer, P.; Haltmeier, M.; Berer, T.; Leiss-Holzinger, E.; Murray, T. W.

2017-07-01

We present an imaging method that uses the random optical speckle patterns that naturally emerge as light propagates through strongly scattering media as a structured illumination source for photoacoustic imaging. Our approach, termed blind structured illumination photoacoustic microscopy (BSIPAM), was inspired by recent work in fluorescence microscopy where super-resolution imaging was demonstrated using multiple unknown speckle illumination patterns. We extend this concept to the multiple scattering domain using photoacoustics (PA), with the speckle pattern serving to generate ultrasound. The optical speckle pattern that emerges as light propagates through diffuse media provides structured illumination to an object placed behind a scattering wall. The photoacoustic signal produced by such illumination is detected using a focused ultrasound transducer. We demonstrate through both simulation and experiment, that by acquiring multiple photoacoustic images, each produced by a different random and unknown speckle pattern, an image of an absorbing object can be reconstructed with a spatial resolution far exceeding that of the ultrasound transducer. We experimentally and numerically demonstrate a gain in resolution of more than a factor of two by using multiple speckle illuminations. The variations in the photoacoustic signals generated with random speckle patterns are utilized in BSIPAM using a novel reconstruction algorithm. Exploiting joint sparsity, this algorithm is capable of reconstructing the absorbing structure from measured PA signals with a resolution close to the speckle size. Another way to excite random excitation for photoacoustic imaging are small absorbing particles, including contrast agents, which flow through small vessels. For such a set-up, the joint-sparsity is generated by the fact that all the particles move in the same vessels. Structured illumination in that case is not necessary.

Analysis of strawberry ripening by dynamic speckle measurements

NASA Astrophysics Data System (ADS)

Mulone, C.; Budini, N.; Vincitorio, F. M.; Freyre, C.; López Díaz, A. J.; Ramil Rego, A.

2013-11-01

This work seeks to determine the age of a fruit from observation of its dynamic speckle pattern. A mobile speckle pattern originates on the fruit's surface due to the interference of the wavefronts reflected from moving scatterers. For this work we analyzed two series of photographs of a strawberry speckle pattern, at different stages of ripening, acquired with a CMOS camera. The first day, we took ten photographs at an interval of one second. The same procedure was repeated the next day. From each series of images we extracted several statistical descriptors of pixel-to-pixel gray level variation during the observation time. By comparing these values from the first to the second day we noticed a diminution of the speckle activity. This decay demonstrated that after only one day the ripening process of the strawberry can be detected by dynamic speckle pattern analysis. For this study we employed a simple new algorithm to process the data obtained from the photographs. This algorithm allows defining a global mobility index that indicates the evolution of the fruit's ripening.

Single-shot speckle reduction in numerical reconstruction of digitally recorded holograms.

PubMed

Hincapie, Diego; Herrera-Ramírez, Jorge; Garcia-Sucerquia, Jorge

2015-04-15

A single-shot method to reduce the speckle noise in the numerical reconstructions of electronically recorded holograms is presented. A recorded hologram with the dimensions N×M is split into S=T×T sub-holograms. The uncorrelated superposition of the individually reconstructed sub-holograms leads to an image with the speckle noise reduced proportionally to the 1/S law. The experimental results are presented to support the proposed methodology.

Speckle-based three-dimensional velocity measurement using spatial filtering velocimetry.

PubMed

Iversen, Theis F Q; Jakobsen, Michael L; Hanson, Steen G

2011-04-10

We present an optical method for measuring the real-time three-dimensional (3D) translational velocity of a diffusely scattering rigid object observed through an imaging system. The method is based on a combination of the motion of random speckle patterns and regular fringe patterns. The speckle pattern is formed in the observation plane of the imaging system due to reflection from an area of the object illuminated by a coherent light source. The speckle pattern translates in response to in-plane translation of the object, and the presence of an angular offset reference wave coinciding with the speckle pattern in the observation plane gives rise to interference, resulting in a fringe pattern that translates in response to the out-of-plane translation of the object. Numerical calculations are performed to evaluate the dynamic properties of the intensity distribution and the response of realistic spatial filters designed to measure the three components of the object's translational velocity. Furthermore, experimental data are presented that demonstrate full 3D velocity measurement. © 2011 Optical Society of America

A Modal Analysis of Submerged Composite Plates Using Digital Speckle Pattern Interferometry

DTIC Science & Technology

1991-05-01

the drive point. The underwater mode shapes were slightly deformed compared to the in- air modes which is probably due to modal coupling by the dense...modes according to Leissa. The mode shapes in water are very similar to those in air with a small amount of distortion due to modal coupling by the fluid...and cantilever boundarv conditions is described in this thesis. The vibrations of the plates are studies in air and when Submerged in a water tank to

Noninvasive, three-dimensional full-field body sensor for surface deformation monitoring of human body in vivo.

PubMed

Chen, Zhenning; Shao, Xinxing; He, Xiaoyuan; Wu, Jialin; Xu, Xiangyang; Zhang, Jinlin

2017-09-01

Noninvasive, three-dimensional (3-D), full-field surface deformation measurements of the human body are important for biomedical investigations. We proposed a 3-D noninvasive, full-field body sensor based on stereo digital image correlation (stereo-DIC) for surface deformation monitoring of the human body in vivo. First, by applying an improved water-transfer printing (WTP) technique to transfer optimized speckle patterns onto the skin, the body sensor was conveniently and harmlessly fabricated directly onto the human body. Then, stereo-DIC was used to achieve 3-D noncontact and noninvasive surface deformation measurements. The accuracy and efficiency of the proposed body sensor were verified and discussed by considering different complexions. Moreover, the fabrication of speckle patterns on human skin, which has always been considered a challenging problem, was shown to be feasible, effective, and harmless as a result of the improved WTP technique. An application of the proposed stereo-DIC-based body sensor was demonstrated by measuring the pulse wave velocity of human carotid artery. (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal.

PubMed

Li, Chenxi; Wang, Ruikang

2017-04-01

We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes.

Complementary Speckle Patterns: Deterministic Interchange of Intrinsic Vortices and Maxima through Scattering Media.

PubMed

Gateau, Jérôme; Rigneault, Hervé; Guillon, Marc

2017-01-27

Intensity maxima and zeros of speckle patterns obtained behind a diffuser are experimentally interchanged by applying a spiral phase delay of charge ±1 to the impinging coherent beam. This transform arises from the expectation that tightly focused beams, which have a planar wave front around the focus, are so changed into vortex beams and vice versa. The statistics of extrema locations and the intensity distribution of the so-generated "complementary" patterns are characterized by numerical simulations. It is demonstrated experimentally that the incoherent superposition of the three "complementary speckle patterns" yield a synthetic speckle grain size enlarged by a factor of sqrt[3]. A cyclic permutation of optical vortices and intensity maxima is unexpectedly observed and discussed.

Cellular organization of pre-mRNA splicing factors in several tissues. Changes in the uterus by hormone action.

PubMed

George-Téllez, R; Segura-Valdez, M L; González-Santos, L; Jiménez-García, L F

2002-05-01

In the mammalian cell nucleus, splicing factors are distributed in nuclear domains known as speckles or splicing factor compartments (SFCs). In cultured cells, these domains are dynamic and reflect transcriptional and splicing activities. We used immunofluorescence and confocal microscopy to monitor whether splicing factors in differentiated cells display similar features. Speckled patterns are observed in rat hepatocytes, beta-cells, bronchial and intestine epithelia and also in three cell types of the uterus. Moreover, the number, distribution and sizes of the speckles vary among them. In addition, we studied variations in the circular form (shape) of speckles in uterine cells that are transcriptionally modified by a hormone action. During proestrus of the estral cycle, speckles are irregular in shape while in diestrus I they are circular. Experimentally, in castrated rats luminal epithelial cells show a pattern where speckles are dramatically rounded, but they recover their irregular shape rapidly after an injection of estradiol. The same results were observed in muscle and gland epithelial cells of the uterus. We concluded that different speckled patterns are present in various cells types in differentiated tissues and that these patterns change in the uterus depending upon the presence or absence of hormones such as estradiol.

Development of Michelson interferometer based spatial phase-shift digital shearography

NASA Astrophysics Data System (ADS)

Xie, Xin

Digital shearography is a non-contact, full field, optical measurement method, which has the capability of directly measuring the gradient of deformation. For high measurement sensitivity, phase evaluation method has to be introduced into digital shearography by phase-shift technique. Catalog by phase-shift method, digital phase-shift shearography can be divided into Temporal Phase-Shift Digital Shearography (TPS-DS) and Spatial Phase-Shift Digital Shearography (SPS-DS). TPS-DS is the most widely used phase-shift shearography system, due to its simple algorithm, easy operation and good phase-map quality. However, the application of TPS-DS is only limited in static/step-by-step loading measurement situation, due to its multi-step shifting process. In order to measure the strain under dynamic/continuous loading situation, a SPS-DS system has to be developed. This dissertation aims to develop a series of Michelson Interferometer based SPS-DS measurement methods to achieve the strain measurement by using only a single pair of speckle pattern images. The Michelson Interferometer based SPS-DS systems utilize special designed optical setup to introduce extra carrier frequency into the laser wavefront. The phase information corresponds to the strain field can be separated on the Fourier domain using a Fourier Transform and can further be evaluated with a Windowed Inverse Fourier Transform. With different optical setups and carrier frequency arrangements, the Michelson Interferometer based SPS-DS method is capable to achieve a variety of measurement tasks using only single pair of speckle pattern images. Catalog by the aimed measurand, these capable measurement tasks can be divided into five categories: 1) measurement of out-of-plane strain field with small shearing amount; 2) measurement of relative out-of-plane deformation field with big shearing amount; 3) simultaneous measurement of relative out-of-plane deformation field and deformation gradient field by using multiple carrier frequencies; 4) simultaneous measurement of two directional strain field using dual measurement channels 5) measurement of pure in-plane strain and pure out-of-plane strain with multiple carrier frequencies. The basic theory, optical path analysis, preliminary studies, results analysis and research plan are shown in detail in this dissertation.

Accurate reconstruction in digital holographic microscopy using Fresnel dual-tree complex wavelet transform

NASA Astrophysics Data System (ADS)

Zhang, Xiaolei; Zhang, Xiangchao; Yuan, He; Zhang, Hao; Xu, Min

2018-02-01

Digital holography is a promising measurement method in the fields of bio-medicine and micro-electronics. But the captured images of digital holography are severely polluted by the speckle noise because of optical scattering and diffraction. Via analyzing the properties of Fresnel diffraction and the topographies of micro-structures, a novel reconstruction method based on the dual-tree complex wavelet transform (DT-CWT) is proposed. This algorithm is shiftinvariant and capable of obtaining sparse representations for the diffracted signals of salient features, thus it is well suited for multiresolution processing of the interferometric holograms of directional morphologies. An explicit representation of orthogonal Fresnel DT-CWT bases and a specific filtering method are developed. This method can effectively remove the speckle noise without destroying the salient features. Finally, the proposed reconstruction method is compared with the conventional Fresnel diffraction integration and Fresnel wavelet transform with compressive sensing methods to validate its remarkable superiority on the aspects of topography reconstruction and speckle removal.

Measurement of impinging butane flame using combined optical system with digital speckle tomography

NASA Astrophysics Data System (ADS)

Ko, Han Seo; Ahn, Seong Soo; Kim, Hyun Jung

2011-11-01

Three-dimensional density distributions of an impinging and eccentric flame were measured experimentally using a combined optical system with digital speckle tomography. In addition, a three-dimensional temperature distribution of the flame was reconstructed from an ideal gas equation based on the reconstructed density data. The flame was formed by the ignition of premixed butane/air from air holes and impinged upward against a plate located 24 mm distance from the burner nozzle. In order to verify the reconstruction process for the experimental measurements, numerically synthesized phantoms of impinging and eccentric flames were derived and reconstructed using a developed three-dimensional multiplicative algebraic reconstruction technique (MART). A new scanning technique was developed for the accurate analysis of speckle displacements necessary for investigating the wall jet regions of the impinging flame at which a sharp variation of the flow direction and pressure gradient occur. The reconstructed temperatures by the digital speckle tomography were applied to the boundary condition for numerical analysis of a flame impinged plate. Then, the numerically calculated temperature distribution of the upper side of the flame impinged plate was compared to temperature data taken by an infrared camera. The absolute average uncertainty between the numerical and infrared camera data was 3.7%.

Speckle suppression by doubly scattering systems.

PubMed

Li, Dayan; Kelly, Damien P; Sheridan, John T

2013-12-10

Speckle suppression in a two-diffuser system is examined. An analytical expression for the speckle space-time correlation function is derived, so that the speckle suppression mechanism can be investigated statistically. The grain size of the speckle field illuminating the second diffuser has a major impact on the speckle contrast after temporal averaging. It is shown that, when both the diffusers are rotating, the one with the lower rotating speed determines the period of the speckle correlation function. The coherent length of the averaged speckle intensity is shown to equal the mean speckle size of the individual speckle pattern before averaging. Numerical and experimental results are presented to verify our analysis in the context of speckle reduction.

Applications of polarization speckle in skin cancer detection and monitoring

NASA Astrophysics Data System (ADS)

Lee, Tim K.; Tchvialeva, Lioudmila; Phillips, Jamie; Louie, Daniel C.; Zhao, Jianhua; Wang, Wei; Lui, Harvey; Kalia, Sunil

2018-01-01

Polarization speckle is a rapidly developed field. Unlike laser speckle, polarization speckle consists of stochastic interference patterns with spatially random polarizations, amplitudes and phases. We have been working in this exciting research field, developing techniques to generate polarization patterns from skin. We hypothesize that polarization speckle patterns could be used in biomedical applications, especially, for detecting and monitoring skin cancers, the most common neoplasmas for white populations around the world. This paper describes our effort in developing two polarization speckle devices. One of them captures the Stokes parameters So and S1 simultaneously, and another one captures all four Stokes parameters So, S1, S2, and S3 in one-shot, within milliseconds. Hence these two devices could be used in medical clinics and assessed skin conditions in-vivo. In order to validate our hypothesis, we conducted a series of three clinical studies. These are early pilot studies, and the results suggest that the devices have potential to detect and monitor skin cancers.

Portable laser speckle perfusion imaging system based on digital signal processor.

PubMed

Tang, Xuejun; Feng, Nengyun; Sun, Xiaoli; Li, Pengcheng; Luo, Qingming

2010-12-01

The ability to monitor blood flow in vivo is of major importance in clinical diagnosis and in basic researches of life science. As a noninvasive full-field technique without the need of scanning, laser speckle contrast imaging (LSCI) is widely used to study blood flow with high spatial and temporal resolution. Current LSCI systems are based on personal computers for image processing with large size, which potentially limit the widespread clinical utility. The need for portable laser speckle contrast imaging system that does not compromise processing efficiency is crucial in clinical diagnosis. However, the processing of laser speckle contrast images is time-consuming due to the heavy calculation for enormous high-resolution image data. To address this problem, a portable laser speckle perfusion imaging system based on digital signal processor (DSP) and the algorithm which is suitable for DSP is described. With highly integrated DSP and the algorithm, we have markedly reduced the size and weight of the system as well as its energy consumption while preserving the high processing speed. In vivo experiments demonstrate that our portable laser speckle perfusion imaging system can obtain blood flow images at 25 frames per second with the resolution of 640 × 480 pixels. The portable and lightweight features make it capable of being adapted to a wide variety of application areas such as research laboratory, operating room, ambulance, and even disaster site.

Fundamental uncertainty limit for speckle displacement measurements.

PubMed

Fischer, Andreas

2017-09-01

The basic metrological task in speckle photography is to quantify displacements of speckle patterns, allowing for instance the investigation of the mechanical load and modification of objects with rough surfaces. However, the fundamental limit of the measurement uncertainty due to photon shot noise is unknown. For this reason, the Cramér-Rao bound (CRB) is derived for speckle displacement measurements, representing the squared minimal achievable measurement uncertainty. As result, the CRB for speckle patterns is only two times the CRB for an ideal point light source. Hence, speckle photography is an optimal measurement approach for contactless displacement measurements on rough surfaces. In agreement with a derivation from Heisenberg's uncertainty principle, the CRB depends on the number of detected photons and the diffraction limit of the imaging system described by the speckle size. The theoretical results are verified and validated, demonstrating the capability for displacement measurements with nanometer resolution.

Speckle lithography for fabricating Gaussian, quasi-random 2D structures and black silicon structures.

PubMed

Bingi, Jayachandra; Murukeshan, Vadakke Matham

2015-12-18

Laser speckle pattern is a granular structure formed due to random coherent wavelet interference and generally considered as noise in optical systems including photolithography. Contrary to this, in this paper, we use the speckle pattern to generate predictable and controlled Gaussian random structures and quasi-random structures photo-lithographically. The random structures made using this proposed speckle lithography technique are quantified based on speckle statistics, radial distribution function (RDF) and fast Fourier transform (FFT). The control over the speckle size, density and speckle clustering facilitates the successful fabrication of black silicon with different surface structures. The controllability and tunability of randomness makes this technique a robust method for fabricating predictable 2D Gaussian random structures and black silicon structures. These structures can enhance the light trapping significantly in solar cells and hence enable improved energy harvesting. Further, this technique can enable efficient fabrication of disordered photonic structures and random media based devices.

Statistical spatial properties of speckle patterns generated by multiple laser beams

DOE Office of Scientific and Technical Information (OSTI.GOV)

Le Cain, A.; Sajer, J. M.; Riazuelo, G.

2011-08-15

This paper investigates hot spot characteristics generated by the superposition of multiple laser beams. First, properties of speckle statistics are studied in the context of only one laser beam by computing the autocorrelation function. The case of multiple laser beams is then considered. In certain conditions, it is shown that speckles have an ellipsoidal shape. Analytical expressions of hot spot radii generated by multiple laser beams are derived and compared to numerical estimates made from the autocorrelation function. They are also compared to numerical simulations performed within the paraxial approximation. Excellent agreement is found for the speckle width as wellmore » as for the speckle length. Application to the speckle patterns generated in the Laser MegaJoule configuration in the zone where all the beams overlap is presented. Influence of polarization on the size of the speckles as well as on their abundance is studied.« less

Vibrating Optical Fibers to Make Laser Speckle Disappear

NASA Technical Reports Server (NTRS)

McGill, Matthew; Scott, V. Stanley

2005-01-01

In optical systems in which laser illumination is delivered via multimode optical fibers, laser speckle can be rendered incoherent by a simple but highly effective technique. The need to eliminate speckle arises because speckle can make it difficult to observe edges and other sharp features, thereby making it difficult to perform precision alignment of optical components. The basic ideas of the technique is to vibrate the optical fiber(s) to cause shifting of electromagnetic modes within the fiber(s) and consequent shifting of the speckle pattern in the light emerging from the fiber(s). If the frequency of vibration is high enough, a human eye cannot follow the shifting speckle pattern, so that instead of speckle, a human observer sees a smoothed pattern of light corresponding to a mixture of many electromagnetic modes. If necessary, the optical fiber(s) could be vibrated manually. However, in a typical laboratory situation, it would be more practical to attach a vibrating mechanism to the fiber(s) for routine use as part of the fiber-optic illuminator. In experiments, a commercially available small, gentle, quiet, variable- speed vibratory device was used in this way, with the result that the appearance of speckle was eliminated, as expected. Figures 1 and 2 illustrate the difference.

Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal

PubMed Central

Li, Chenxi; Wang, Ruikang

2017-01-01

Abstract. We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes. PMID:28384709

High-speed single-shot optical focusing through dynamic scattering media with full-phase wavefront shaping.

PubMed

Hemphill, Ashton S; Shen, Yuecheng; Liu, Yan; Wang, Lihong V

2017-11-27

In biological applications, optical focusing is limited by the diffusion of light, which prevents focusing at depths greater than ∼1 mm in soft tissue. Wavefront shaping extends the depth by compensating for phase distortions induced by scattering and thus allows for focusing light through biological tissue beyond the optical diffusion limit by using constructive interference. However, due to physiological motion, light scattering in tissue is deterministic only within a brief speckle correlation time. In in vivo tissue, this speckle correlation time is on the order of milliseconds, and so the wavefront must be optimized within this brief period. The speed of digital wavefront shaping has typically been limited by the relatively long time required to measure and display the optimal phase pattern. This limitation stems from the low speeds of cameras, data transfer and processing, and spatial light modulators. While binary-phase modulation requiring only two images for the phase measurement has recently been reported, most techniques require at least three frames for the full-phase measurement. Here, we present a full-phase digital optical phase conjugation method based on off-axis holography for single-shot optical focusing through scattering media. By using off-axis holography in conjunction with graphics processing unit based processing, we take advantage of the single-shot full-phase measurement while using parallel computation to quickly reconstruct the phase map. With this system, we can focus light through scattering media with a system latency of approximately 9 ms, on the order of the in vivo speckle correlation time.

High-speed single-shot optical focusing through dynamic scattering media with full-phase wavefront shaping

NASA Astrophysics Data System (ADS)

Hemphill, Ashton S.; Shen, Yuecheng; Liu, Yan; Wang, Lihong V.

2017-11-01

In biological applications, optical focusing is limited by the diffusion of light, which prevents focusing at depths greater than ˜1 mm in soft tissue. Wavefront shaping extends the depth by compensating for phase distortions induced by scattering and thus allows for focusing light through biological tissue beyond the optical diffusion limit by using constructive interference. However, due to physiological motion, light scattering in tissue is deterministic only within a brief speckle correlation time. In in vivo tissue, this speckle correlation time is on the order of milliseconds, and so the wavefront must be optimized within this brief period. The speed of digital wavefront shaping has typically been limited by the relatively long time required to measure and display the optimal phase pattern. This limitation stems from the low speeds of cameras, data transfer and processing, and spatial light modulators. While binary-phase modulation requiring only two images for the phase measurement has recently been reported, most techniques require at least three frames for the full-phase measurement. Here, we present a full-phase digital optical phase conjugation method based on off-axis holography for single-shot optical focusing through scattering media. By using off-axis holography in conjunction with graphics processing unit based processing, we take advantage of the single-shot full-phase measurement while using parallel computation to quickly reconstruct the phase map. With this system, we can focus light through scattering media with a system latency of approximately 9 ms, on the order of the in vivo speckle correlation time.

Kitt Peak speckle camera

NASA Technical Reports Server (NTRS)

Breckinridge, J. B.; Mcalister, H. A.; Robinson, W. G.

1979-01-01

The speckle camera in regular use at Kitt Peak National Observatory since 1974 is described in detail. The design of the atmospheric dispersion compensation prisms, the use of film as a recording medium, the accuracy of double star measurements, and the next generation speckle camera are discussed. Photographs of double star speckle patterns with separations from 1.4 sec of arc to 4.7 sec of arc are shown to illustrate the quality of image formation with this camera, the effects of seeing on the patterns, and to illustrate the isoplanatic patch of the atmosphere.

Wholefield displacement measurements using speckle image processing techniques for crash tests

NASA Astrophysics Data System (ADS)

Sriram, P.; Hanagud, S.; Ranson, W. F.

The digital correlation scheme of Peters et al. (1983) was extended to measure out-of-plane deformations, using a white light projection speckle technique. A simple ray optic theory and the digital correlation scheme are outlined. The technique was applied successfully to measure out-of-plane displacements of initially flat rotorcraft structures (an acrylic circular plate and a steel cantilever beam), using a low cost video camera and a desktop computer. The technique can be extended to measurements of three-dimensional deformations and dynamic deformations.

Color speckle in laser displays

NASA Astrophysics Data System (ADS)

Kuroda, Kazuo

2015-07-01

At the beginning of this century, lighting technology has been shifted from discharge lamps, fluorescent lamps and electric bulbs to solid-state lighting. Current solid-state lighting is based on the light emitting diodes (LED) technology, but the laser lighting technology is developing rapidly, such as, laser cinema projectors, laser TVs, laser head-up displays, laser head mounted displays, and laser headlamps for motor vehicles. One of the main issues of laser displays is the reduction of speckle noise1). For the monochromatic laser light, speckle is random interference pattern on the image plane (retina for human observer). For laser displays, RGB (red-green-blue) lasers form speckle patterns independently, which results in random distribution of chromaticity, called color speckle2).

Laser-induced speckle scatter patterns in Bacillus colonies

PubMed Central

Kim, Huisung; Singh, Atul K.; Bhunia, Arun K.; Bae, Euiwon

2014-01-01

Label-free bacterial colony phenotyping technology called BARDOT (Bacterial Rapid Detection using Optical scattering Technology) provided successful classification of several different bacteria at the genus, species, and serovar level. Recent experiments with colonies of Bacillus species provided strikingly different characteristics of elastic light scatter (ELS) patterns, which were comprised of random speckles compared to other bacteria, which are dominated by concentric rings and spokes. Since this laser-based optical sensor interrogates the whole volume of the colony, 3-D information of micro- and macro-structures are all encoded in the far-field scatter patterns. Here, we present a theoretical model explaining the underlying mechanism of the speckle formation by the colonies from Bacillus species. Except for Bacillus polymyxa, all Bacillus spp. produced random bright spots on the imaging plane, which presumably dependent on the cellular and molecular organization and content within the colony. Our scatter model-based analysis revealed that colony spread resulting in variable surface roughness can modify the wavefront of the scatter field. As the center diameter of the Bacillus spp. colony grew from 500 to 900 μm, average speckles area decreased two-fold and the number of small speckles increased seven-fold. In conclusion, as Bacillus colony grows, the average speckle size in the scatter pattern decreases and the number of smaller speckle increases due to the swarming growth characteristics of bacteria within the colony. PMID:25352840

Scattering of Gaussian Beams by Disordered Particulate Media

NASA Technical Reports Server (NTRS)

Mishchenko, Michael I.; Dlugach, Janna M.

2016-01-01

A frequently observed characteristic of electromagnetic scattering by a disordered particulate medium is the absence of pronounced speckles in angular patterns of the scattered light. It is known that such diffuse speckle-free scattering patterns can be caused by averaging over randomly changing particle positions and/or over a finite spectral range. To get further insight into the possible physical causes of the absence of speckles, we use the numerically exact superposition T-matrix solver of the Maxwell equations and analyze the scattering of plane-wave and Gaussian beams by representative multi-sphere groups. We show that phase and amplitude variations across an incident Gaussian beam do not serve to extinguish the pronounced speckle pattern typical of plane-wave illumination of a fixed multi-particle group. Averaging over random particle positions and/or over a finite spectral range is still required to generate the classical diffuse speckle-free regime.

Using of methods of speckle optics for Chlamydia trachomatis typing

NASA Astrophysics Data System (ADS)

Ulyanov, Sergey S.; Zaytsev, Sergey S.; Ulianova, Onega V.; Saltykov, Yury V.; Feodorova, Valentina A.

2017-03-01

Specific method of transformation of nucleotide of gene into speckle pattern is suggested. Reference speckle pattern of omp1 gene of typical wild strains of Chlamydia trachomatis of genovars D, E, F, G, J and K and Chlamydia psittaci as well is generated. Perspectives of proposed technique in the gene identification and detection of natural genetic mutations as single nucleotide polymorphism (SNP) are demonstrated.

Disorderly Light.

ERIC Educational Resources Information Center

Peterson, Ivars

1991-01-01

The relationship between theories about electrical conductivity in microscopic wires and laser speckle patterns is described. Practical applications of laser speckle patterns are included. Wave ideas are being used to describe and predict novel phenomena in disordered solids. (KR)

Speckle lithography for fabricating Gaussian, quasi-random 2D structures and black silicon structures

PubMed Central

Bingi, Jayachandra; Murukeshan, Vadakke Matham

2015-01-01

Laser speckle pattern is a granular structure formed due to random coherent wavelet interference and generally considered as noise in optical systems including photolithography. Contrary to this, in this paper, we use the speckle pattern to generate predictable and controlled Gaussian random structures and quasi-random structures photo-lithographically. The random structures made using this proposed speckle lithography technique are quantified based on speckle statistics, radial distribution function (RDF) and fast Fourier transform (FFT). The control over the speckle size, density and speckle clustering facilitates the successful fabrication of black silicon with different surface structures. The controllability and tunability of randomness makes this technique a robust method for fabricating predictable 2D Gaussian random structures and black silicon structures. These structures can enhance the light trapping significantly in solar cells and hence enable improved energy harvesting. Further, this technique can enable efficient fabrication of disordered photonic structures and random media based devices. PMID:26679513

Multiscale analysis of the intensity fluctuation in a time series of dynamic speckle patterns.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2007-04-10

We propose the application of a method based on the discrete wavelet transform to detect, identify, and measure scaling behavior in dynamic speckle. The multiscale phenomena presented by a sample and displayed by its speckle activity are analyzed by processing the time series of dynamic speckle patterns. The scaling analysis is applied to the temporal fluctuation of the speckle intensity and also to the two derived data sets generated by its magnitude and sign. The application of the method is illustrated by analyzing paint-drying processes and bruising in apples. The results are discussed taking into account the different time organizations obtained for the scaling behavior of the magnitude and the sign of the intensity fluctuation.

Strain and mechanical properties of the VCM multilayer sheet and their composites using the digital speckle correlation method.

PubMed

Zhang, Dehai; Xie, Guizhong; Li, Yanqin; Liu, Jianxiu

2015-09-01

The digital speckle correlation method (DSCM) is introduced to solve the challenging problems in the related geometric measurement. Theoretical calculations of strain are deduced using the DSCM. Corresponding strains along x and y directions are obtained from uniaxial tension experiments and digital speckle measurements, using the VCM nondeep drawing multilayer sheet, the VCM deep-drawing multilayer sheet, clad films, nondeep drawing substrate, and deep-drawing substrate sheet as the targeted experimental objects. The results show that the maximum strains along the x direction of the VCM nondeep drawing multilayer sheet, the VCM deep-drawing multilayer sheet, clad film, nondeep drawing substrate, and deep-drawing substrate sheet are 68.473%, 48.632%, 91.632%, 50.784% and 40.068%, respectively, while the maximum strains along the y direction are -2.657%, -15.381%, 2.826%, -9.780% and -7.783%, respectively. The mechanical properties of the VCM multilayer sheet are between those of the substrate and clad film, while mechanical properties of the VCM deep-drawing multilayer sheet are superior to those of the VCM nondeep drawing multi-layer sheet.

Optical measurement of sound using time-varying laser speckle patterns

NASA Astrophysics Data System (ADS)

Leung, Terence S.; Jiang, Shihong; Hebden, Jeremy

2011-02-01

In this work, we introduce an optical technique to measure sound. The technique involves pointing a coherent pulsed laser beam on the surface of the measurement site and capturing the time-varying speckle patterns using a CCD camera. Sound manifests itself as vibrations on the surface which induce a periodic translation of the speckle pattern over time. Using a parallel speckle detection scheme, the dynamics of the time-varying speckle patterns can be captured and processed to produce spectral information of the sound. One potential clinical application is to measure pathological sounds from the brain as a screening test. We performed experiments to demonstrate the principle of the detection scheme using head phantoms. The results show that the detection scheme can measure the spectra of single frequency sounds between 100 and 2000 Hz. The detection scheme worked equally well in both a flat geometry and an anatomical head geometry. However, the current detection scheme is too slow for use in living biological tissues which has a decorrelation time of a few milliseconds. Further improvements have been suggested.

Digital Image Correlation of Concrete Slab at University of Tennessee, Knoxville

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mahadevan, Sankaran; Agarwal, Vivek; Pham, Binh T.

Assessment and management of aging concrete structures in nuclear power plants require a more systematic approach than simple reliance on existing code margins of safety. Some degradation mechanisms of concrete manifest themselves via swelling or by other shape deformation of the concrete. Specifically, degradation of concrete structure damaged by ASR is viewed as one of the dominant factors impacting the structural integrity of aging nuclear power plants. Structural health monitoring of concrete structures aims to understand the current health condition of a structure based on heterogeneous measurements to produce high-confidence actionable information regarding structural integrity that supports operational and maintenancemore » decisions. Number of nondestructive examination techniques (i.e., thermography, digital image correlation, mechanical deformation measurements, nonlinear impact resonance (DIC) acoustic spectroscopy, and vibro-acoustic modulation) is used to detect the damage caused by ASR. DIC techniques have been increasing in popularity, especially in micro- and nano-scale mechanical testing applications due to its relative ease of implementation and use. Advances in computer technology and digital cameras help this method moving forward. To ensure the best outcome of the DIC system, important factors in the experiment are identified. They include standoff distance, speckle size, speckle pattern, and durable paint. These optimal experimental options are selected basing on a thorough investigation. The resulting DIC deformation map indicates that this technique can be used to generate data related to degradation assessment of concrete structure damaged by the impact of ASR.« less

Surface modeling method for aircraft engine blades by using speckle patterns based on the virtual stereo vision system

NASA Astrophysics Data System (ADS)

Yu, Zhijing; Ma, Kai; Wang, Zhijun; Wu, Jun; Wang, Tao; Zhuge, Jingchang

2018-03-01

A blade is one of the most important components of an aircraft engine. Due to its high manufacturing costs, it is indispensable to come up with methods for repairing damaged blades. In order to obtain a surface model of the blades, this paper proposes a modeling method by using speckle patterns based on the virtual stereo vision system. Firstly, blades are sprayed evenly creating random speckle patterns and point clouds from blade surfaces can be calculated by using speckle patterns based on the virtual stereo vision system. Secondly, boundary points are obtained in the way of varied step lengths according to curvature and are fitted to get a blade surface envelope with a cubic B-spline curve. Finally, the surface model of blades is established with the envelope curves and the point clouds. Experimental results show that the surface model of aircraft engine blades is fair and accurate.

Simultaneous measurement of translation and tilt using digital speckle photography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bhaduri, Basanta; Quan, Chenggen; Tay, Cho Jui

2010-06-20

A Michelson-type digital speckle photographic system has been proposed in which one light beam produces a Fourier transform and another beam produces an image at a recording plane, without interfering between themselves. Because the optical Fourier transform is insensitive to translation and the imaging technique is insensitive to tilt, the proposed system is able to simultaneously and independently determine both surface tilt and translation by two separate recordings, one before and another after the surface motion, without the need to obtain solutions for simultaneous equations. Experimental results are presented to verify the theoretical analysis.

Digital processing of orbital radar data to enhance geologic structure - Examples from the Canadian Shield

NASA Technical Reports Server (NTRS)

Masuoka, Penny M.; Harris, Jeff; Lowman, Paul D., Jr.; Blodget, Herbert W.

1988-01-01

Various digital enhancement techniques for SAR are compared using SIR-B and Seasat images of the Canadian Shield. The three best methods for enhancing geological structure were found to be: (1) a simple linear contrast stretch; (2) a mean or median low-pass filter to reduce speckle prior to edge enhancement or a K nearest-neighbor average to cosmetically reduce speckle; and (3) a modification of the Moore-Waltz (1983) technique. Three look directions were coregistered and several means of data display were investigated as means of compensating for radar azimuth biasing.

Reducing Speckle In One-Look SAR Images

NASA Technical Reports Server (NTRS)

Nathan, K. S.; Curlander, J. C.

1990-01-01

Local-adaptive-filter algorithm incorporated into digital processing of synthetic-aperture-radar (SAR) echo data to reduce speckle in resulting imagery. Involves use of image statistics in vicinity of each picture element, in conjunction with original intensity of element, to estimate brightness more nearly proportional to true radar reflectance of corresponding target. Increases ratio of signal to speckle noise without substantial degradation of resolution common to multilook SAR images. Adapts to local variations of statistics within scene, preserving subtle details. Computationally simple. Lends itself to parallel processing of different segments of image, making possible increased throughput.

Archeological Echocardiography: Digitization and Speckle Tracking Analysis of Archival Echocardiograms in the HyperGEN Study.

PubMed

Aguilar, Frank G; Selvaraj, Senthil; Martinez, Eva E; Katz, Daniel H; Beussink, Lauren; Kim, Kwang-Youn A; Ping, Jie; Rasmussen-Torvik, Laura; Goyal, Amita; Sha, Jin; Irvin, Marguerite R; Arnett, Donna K; Shah, Sanjiv J

2016-03-01

Several large epidemiologic studies and clinical trials have included echocardiography, but images were stored in analog format and these studies predated tissue Doppler imaging (TDI) and speckle tracking echocardiography (STE). We hypothesized that digitization of analog echocardiograms, with subsequent quantification of cardiac mechanics using STE, is feasible, reproducible, accurate, and produces clinically valid results. In the NHLBI HyperGEN study (N = 2234), archived analog echocardiograms were digitized and subsequently analyzed using STE to obtain tissue velocities/strain. Echocardiograms were assigned quality scores and inter-/intra-observer agreement was calculated. Accuracy was evaluated in: (1) a separate second study (N = 50) comparing prospective digital strain versus post hoc analog-to-digital strain, and (2) in a third study (N = 95) comparing prospectively obtained TDI e' velocities with post hoc STE e' velocities. Finally, we replicated previously known associations between tissue velocities/strain, conventional echocardiographic measurements, and clinical data. Of the 2234 HyperGEN echocardiograms, 2150 (96.2%) underwent successful digitization and STE analysis. Inter/intra-observer agreement was high for all STE parameters, especially longitudinal strain (LS). In accuracy studies, LS performed best when comparing post hoc STE to prospective digital STE for strain analysis. STE-derived e' velocities correlated with, but systematically underestimated, TDI e' velocity. Several known associations between clinical variables and cardiac mechanics were replicated in HyperGEN. We also found a novel independent inverse association between fasting glucose and LS (adjusted β = -2.4 [95% CI -3.6, -1.2]% per 1-SD increase in fasting glucose; P < 0.001). Archeological echocardiography, the digitization and speckle tracking analysis of archival echocardiograms, is feasible and generates indices of cardiac mechanics similar to contemporary studies. © 2015, Wiley Periodicals, Inc.

Archeological Echocardiography: Digitization and Speckle-Tracking Analysis of Archival Echocardiograms in the HyperGEN Study

PubMed Central

Aguilar, Frank G.; Selvaraj, Senthil; Martinez, Eva E.; Katz, Daniel H.; Beussink, Lauren; Kim, Kwang-Youn A.; Ping, Jie; Rasmussen-Torvik, Laura; Goyal, Amita; Sha, Jin; Irvin, Marguerite R.; Arnett, Donna K.; Shah, Sanjiv J.

2015-01-01

Background Several large epidemiologic studies and clinical trials have included echocardiography, but images were stored in analog format and these studies predated tissue Doppler imaging (TDI) and speckle-tracking echocardiography (STE). We hypothesized that digitization of analog echocardiograms, with subsequent quantification of cardiac mechanics using STE, is feasible, reproducible, accurate, and produces clinically valid results. Methods In the NHLBI HyperGEN study (N=2234), archived analog echocardiograms were digitized and subsequently analyzed using STE to obtain tissue velocities/strain. Echocardiograms were assigned quality scores and inter/intraobserver agreement was calculated. Accuracy was evaluated in (1) a separate second study (N=50) comparing prospective digital strain vs. post-hoc analog-to-digital strain; and (2) in a third study (N=95) comparing prospectively-obtained TDI e′ velocities with post-hoc STE e′ velocities. Finally, we replicated previously known associations between tissue velocities/strain, conventional echocardiographic measurements, and clinical data. Results Of the 2234 HyperGEN echocardiograms, 2150 (96.2%) underwent successful digitization and STE analysis. Inter/intraobserver agreement was high for all STE parameters, especially longitudinal strain (LS). In accuracy studies, LS performed best when comparing post-hoc STE to prospective digital STE for strain analysis. STE-derived e′ velocities correlated with, but systematically underestimated, TDI e′ velocity. Several known associations between clinical variables and cardiac mechanics were replicated in HyperGEN. We also found a novel independent inverse association between fasting glucose and LS (adjusted β =−2.4 [95% CI −3.6,−1.2]% per 1-SD increase in fasting glucose; P<0.001). Conclusions Archeological echocardiography, the digitization and speckle-tracking analysis of archival echocardiograms, is feasible and generates parameters of cardiac mechanics similar to contemporary studies. PMID:26525308

High-speed imaging using compressed sensing and wavelength-dependent scattering (Conference Presentation)

NASA Astrophysics Data System (ADS)

Shin, Jaewook; Bosworth, Bryan T.; Foster, Mark A.

2017-02-01

The process of multiple scattering has inherent characteristics that are attractive for high-speed imaging with high spatial resolution and a wide field-of-view. A coherent source passing through a multiple-scattering medium naturally generates speckle patterns with diffraction-limited features over an arbitrarily large field-of-view. In addition, the process of multiple scattering is deterministic allowing a given speckle pattern to be reliably reproduced with identical illumination conditions. Here, by exploiting wavelength dependent multiple scattering and compressed sensing, we develop a high-speed 2D time-stretch microscope. Highly chirped pulses from a 90-MHz mode-locked laser are sent through a 2D grating and a ground-glass diffuser to produce 2D speckle patterns that rapidly evolve with the instantaneous frequency of the chirped pulse. To image a scene, we first characterize the high-speed evolution of the generated speckle patterns. Subsequently we project the patterns onto the microscopic region of interest and collect the total light from the scene using a single high-speed photodetector. Thus the wavelength dependent speckle patterns serve as high-speed pseudorandom structured illumination of the scene. An image sequence is then recovered using the time-dependent signal received by the photodetector, the known speckle pattern evolution, and compressed sensing algorithms. Notably, the use of compressed sensing allows for reconstruction of a time-dependent scene using a highly sub-Nyquist number of measurements, which both increases the speed of the imager and reduces the amount of data that must be collected and stored. We will discuss our experimental demonstration of this approach and the theoretical limits on imaging speed.

Optically phase-locked electronic speckle pattern interferometer

NASA Astrophysics Data System (ADS)

Moran, Steven E.; Law, Robert; Craig, Peter N.; Goldberg, Warren M.

1987-02-01

The design, theory, operation, and characteristics of an optically phase-locked electronic speckle pattern interferometer (OPL-ESPI) are described. The OPL-ESPI system couples an optical phase-locked loop with an ESPI system to generate real-time equal Doppler speckle contours of moving objects from unstable sensor platforms. In addition, the optical phase-locked loop provides the basis for a new ESPI video signal processing technique which incorporates local oscillator phase shifting coupled with video sequential frame subtraction.

A projector calibration method for monocular structured light system based on digital image correlation

NASA Astrophysics Data System (ADS)

Feng, Zhixin

2018-02-01

Projector calibration is crucial for a camera-projector three-dimensional (3-D) structured light measurement system, which has one camera and one projector. In this paper, a novel projector calibration method is proposed based on digital image correlation. In the method, the projector is viewed as an inverse camera, and a plane calibration board with feature points is used to calibrate the projector. During the calibration processing, a random speckle pattern is projected onto the calibration board with different orientations to establish the correspondences between projector images and camera images. Thereby, dataset for projector calibration are generated. Then the projector can be calibrated using a well-established camera calibration algorithm. The experiment results confirm that the proposed method is accurate and reliable for projector calibration.

Speckles in interstellar radio-wave scattering

NASA Technical Reports Server (NTRS)

Desai, K. M.; Gwinn, C. R.; Reynolds, J.; King, E. A.; Jauncey, D.; Nicholson, G.; Flanagan, C.; Preston, R. A.; Jones, D. L.

1991-01-01

Observations of speckles in the scattering disk of the Vela pulsar are presented and speckle techniques for studying and circumventing scattering of radio waves by the turbulent interstellar plasma are discussed. The speckle pattern contains, in a hologrammatic fashion, complete information on the structure of the radio source as well as the distribution of the scattering material. Speckle observations of interstellar scattering of radio waves are difficult because of their characteristically short timescales and narrow bandwidths. Here, first observations are presented, taken at 13 cm wavelength with elements of the SHEVE VLBI network, of speckles in interstellar scattering.

Statistics on gene-based laser speckles with a small number of scatterers: implications for the detection of polymorphism in the Chlamydia trachomatis omp1 gene

NASA Astrophysics Data System (ADS)

Ulyanov, Sergey S.; Ulianova, Onega V.; Zaytsev, Sergey S.; Saltykov, Yury V.; Feodorova, Valentina A.

2018-04-01

The transformation mechanism for a nucleotide sequence of the Chlamydia trachomatis gene into a speckle pattern has been considered. The first and second-order statistics of gene-based speckles have been analyzed. It has been demonstrated that gene-based speckles do not obey Gaussian statistics and belong to the class of speckles with a small number of scatterers. It has been shown that gene polymorphism can be easily detected through analysis of the statistical characteristics of gene-based speckles.

Speckle pattern sequential extraction metric for estimating the focus spot size on a remote diffuse target.

PubMed

Yu, Zhan; Li, Yuanyang; Liu, Lisheng; Guo, Jin; Wang, Tingfeng; Yang, Guoqing

2017-11-10

The speckle pattern (line by line) sequential extraction (SPSE) metric is proposed by the one-dimensional speckle intensity level crossing theory. Through the sequential extraction of received speckle information, the speckle metrics for estimating the variation of focusing spot size on a remote diffuse target are obtained. Based on the simulation, we will give some discussions about the SPSE metric range of application under the theoretical conditions, and the aperture size will affect the metric performance of the observation system. The results of the analyses are verified by the experiment. This method is applied to the detection of relative static target (speckled jitter frequency is less than the CCD sampling frequency). The SPSE metric can determine the variation of the focusing spot size over a long distance, moreover, the metric will estimate the spot size under some conditions. Therefore, the monitoring and the feedback of far-field spot will be implemented laser focusing system applications and help the system to optimize the focusing performance.

Effects of speckle/pixel size ratio on temporal and spatial speckle-contrast analysis of dynamic scattering systems: Implications for measurements of blood-flow dynamics.

PubMed

Ramirez-San-Juan, J C; Mendez-Aguilar, E; Salazar-Hermenegildo, N; Fuentes-Garcia, A; Ramos-Garcia, R; Choi, B

2013-01-01

Laser Speckle Contrast Imaging (LSCI) is an optical technique used to generate blood flow maps with high spatial and temporal resolution. It is well known that in LSCI, the speckle size must exceed the Nyquist criterion to maximize the speckle's pattern contrast. In this work, we study experimentally the effect of speckle-pixel size ratio not only in dynamic speckle contrast, but also on the calculation of the relative flow speed for temporal and spatial analysis. Our data suggest that the temporal LSCI algorithm is more accurate at assessing the relative changes in flow speed than the spatial algorithm.

Effects of red blood cell aggregates dissociation on the estimation of ultrasound speckle image velocimetry.

PubMed

Yeom, Eunseop; Nam, Kweon-Ho; Paeng, Dong-Guk; Lee, Sang-Joon

2014-08-01

Ultrasound speckle image of blood is mainly attributed by red blood cells (RBCs) which tend to form RBC aggregates. RBC aggregates are separated into individual cells when the shear force is over a certain value. The dissociation of RBC aggregates has an influence on the performance of ultrasound speckle image velocimetry (SIV) technique in which a cross-correlation algorithm is applied to the speckle images to get the velocity field information. The present study aims to investigate the effect of the dissociation of RBC aggregates on the estimation quality of SIV technique. Ultrasound B-mode images were captured from the porcine blood circulating in a mock-up flow loop with varying flow rate. To verify the measurement performance of SIV technique, the centerline velocity measured by the SIV technique was compared with that measured by Doppler spectrograms. The dissociation of RBC aggregates was estimated by using decorrelation of speckle patterns in which the subsequent window was shifted as much as the speckle displacement to compensate decorrelation caused by in-plane loss of speckle patterns. The decorrelation of speckles is considerably increased according to shear rate. Its variations are different along the radial direction. Because the dissociation of RBC aggregates changes ultrasound speckles, the estimation quality of SIV technique is significantly correlated with the decorrelation of speckles. This degradation of measurement quality may be improved by increasing the data acquisition rate. This study would be useful for simultaneous measurement of hemodynamic and hemorheological information of blood flows using only speckle images. Copyright © 2014 Elsevier B.V. All rights reserved.

Reflectance Speckle of Retinal Nerve Fiber Layer Reveals Axonal Activity

PubMed Central

Huang, Xiang-Run; Knighton, Robert W.; Zhou, Ye; Zhao, Xiao-Peng

2013-01-01

Purpose. This study investigated the retinal nerve fiber layer (RNFL) reflectance speckle and tested the hypothesis that temporal change of RNFL speckle reveals axonal dynamic activity. Methods. RNFL reflectance speckle of isolated rat retinas was studied with monochromatic illumination. A series of reflectance images was collected every 5 seconds for approximately 15 minutes. Correlation coefficients (CC) of selected areas between a reference and subsequent images were calculated and plotted as a function of the time intervals between images. An exponential function fit to the time course was used to evaluate temporal change of speckle pattern. To relate temporal change of speckle to axonal activity, in vitro living retina perfused at a normal (34°C) and a lower (24°C) temperature, paraformaldehyde-fixed retina, and retina treated with microtubule depolymerization were used. Results. RNFL reflectance was not uniform; rather nerve fiber bundles had a speckled texture that changed with time. In normally perfused retina, the time constant of the CC change was 0.56 ± 0.26 minutes. In retinas treated with lower temperature and microtubule depolymerization, the time constants increased by two to four times, indicating that the speckle pattern changed more slowly. The speckled texture in fixed retina was stationary. Conclusions. Fixation stops axonal activity; treatments with either lower temperature or microtubule depolymerization are known to decrease axonal transport. The results obtained in this study suggest that temporal change of RNFL speckle reveals structural change due to axonal activity. Assessment of RNFL reflectance speckle may offer a new means of evaluating axonal function. PMID:23532525

Speckle-modulation for speckle reduction in optical coherence tomography

NASA Astrophysics Data System (ADS)

Liba, Orly; Lew, Matthew D.; SoRelle, Elliott D.; Dutta, Rebecca; Sen, Debasish; Moshfeghi, Darius M.; Chu, Steven; de la Zerda, Adam

2018-02-01

Optical coherence tomography (OCT) is a powerful biomedical imaging technology that relies on the coherent detection of backscattered light to image tissue morphology in vivo. As a consequence, OCT is susceptible to coherent noise, known as speckle noise, which imposes significant limitations on its diagnostic capabilities. Here we show Speckle- Modulating OCT (SM-OCT), a method based purely on light manipulation, which can remove speckle noise, including noise originating from sample multiple back-scattering. SM-OCT accomplishes this by creating and averaging an unlimited number of scans with uncorrelated speckle patterns, without compromising spatial resolution. The uncorrelated speckle patterns are created by scrambling the phase of the light with sub-resolution features using a moving ground-glass diffuser in the optical path of the sample arm. This method can be implemented in existing OCTs as a relatively low-cost add-on. SM-OCT speckle statistics follow the expected decrease in speckle contrast as the number of averaged scans increases. Within a scattering phantom, SM-OCT provides a 2.5-fold increase in effective resolution compared to conventional OCT. Using SM-OCT, we reveal small structures in the tissues of living animals, such as the inner stromal structure of a live mouse cornea, the fine structures inside the mouse pinna, and sweat ducts and Meissner's corpuscle in the human fingertip skin - features that are otherwise obscured by speckle noise when using conventional OCT or OCT with current state of the art speckle reduction methods. Our results indicate that SM-OCT has the potential to improve the current diagnostic and intra-operative capabilities of OCT.

In vivo visualization method by absolute blood flow velocity based on speckle and fringe pattern using two-beam multipoint laser Doppler velocimetry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kyoden, Tomoaki, E-mail: kyouden@nc-toyama.ac.jp; Naruki, Shoji; Akiguchi, Shunsuke

Two-beam multipoint laser Doppler velocimetry (two-beam MLDV) is a non-invasive imaging technique able to provide an image of two-dimensional blood flow and has potential for observing cancer as previously demonstrated in a mouse model. In two-beam MLDV, the blood flow velocity can be estimated from red blood cells passing through a fringe pattern generated in the skin. The fringe pattern is created at the intersection of two beams in conventional LDV and two-beam MLDV. Being able to choose the depth position is an advantage of two-beam MLDV, and the position of a blood vessel can be identified in a three-dimensionalmore » space using this technique. Initially, we observed the fringe pattern in the skin, and the undeveloped or developed speckle pattern generated in a deeper position of the skin. The validity of the absolute velocity value detected by two-beam MLDV was verified while changing the number of layers of skin around a transparent flow channel. The absolute velocity value independent of direction was detected using the developed speckle pattern, which is created by the skin construct and two beams in the flow channel. Finally, we showed the relationship between the signal intensity and the fringe pattern, undeveloped speckle, or developed speckle pattern based on the skin depth. The Doppler signals were not detected at deeper positions in the skin, which qualitatively indicates the depth limit for two-beam MLDV.« less

Vibration analysis based on electronic stroboscopic speckle-shearing pattern interferometry

NASA Astrophysics Data System (ADS)

Jia, Dagong; Yu, Changsong; Xu, Tianhua; Jin, Chao; Zhang, Hongxia; Jing, Wencai; Zhang, Yimo

2008-12-01

In this paper, an electronic speckle-shearing pattern interferometer with pulsed laser and pulse frequency controller is fabricated. The principle of measuring the vibration in the object using electronic stroboscopic speckle--shearing pattern interferometer is analyzed. Using a metal plate, the edge of which is clamped, as an experimental specimen, the shear interferogram are obtained under two experimental frequencies, 100 Hz and 200 Hz. At the same time, the vibration of this metal plate under the same experimental conditions is measured using the time-average method in order to test the performance of this electronic stroboscopic speckle-shearing pattern interferometer. The result indicated that the fringe of shear interferogram become dense with the experimental frequency increasing. Compared the fringe pattern obtained by the stroboscopic method with the fringe obtained by the time-average method, the shearing interferogram of stroboscopic method is clearer than the time-average method. In addition, both the time-average method and stroboscopic method are suited for qualitative analysis for the vibration of the object. More over, the stroboscopic method is well adapted to quantitative vibration analysis.

Active dynamics of colloidal particles in time-varying laser speckle patterns

PubMed Central

Bianchi, Silvio; Pruner, Riccardo; Vizsnyiczai, Gaszton; Maggi, Claudio; Di Leonardo, Roberto

2016-01-01

Colloidal particles immersed in a dynamic speckle pattern experience an optical force that fluctuates both in space and time. The resulting dynamics presents many interesting analogies with a broad class of non-equilibrium systems like: active colloids, self propelled microorganisms, transport in dynamical intracellular environments. Here we show that the use of a spatial light modulator allows to generate light fields that fluctuate with controllable space and time correlations and a prescribed average intensity profile. In particular we generate ring-shaped random patterns that can confine a colloidal particle over a quasi one-dimensional random energy landscape. We find a mean square displacement that is diffusive at both short and long times, while a superdiffusive or subdiffusive behavior is observed at intermediate times depending on the value of the speckles correlation time. We propose two alternative models for the mean square displacement in the two limiting cases of a short or long speckles correlation time. A simple interpolation formula is shown to account for the full phenomenology observed in the mean square displacement across the entire range from fast to slow fluctuating speckles. PMID:27279540

Optical vortices as potential indicators of biophysical dynamics

NASA Astrophysics Data System (ADS)

Majumdar, Anindya; Kirkpatrick, Sean J.

2017-03-01

Laser speckle patterns are granular patterns produced as a result of random interference of light waves. Optical vortices (OVs) are phase singularities in such speckle fields, characterized by zero intensity and an undefined phase. Decorrelation of the speckle fields causes these OVs to move in both time and space. In this work, a variety of parameters of these OVs have been studied. The speckle fields were simulated to undergo three distinct decorrelation behaviors- Gaussian, Lorentzian and constant decorrelations. Different decorrelation behaviors represent different dynamics. For example, Lorentzian and Gaussian decorrelations represent Brownian and ordered motions, respectively. Typical dynamical systems in biophysics are generally argued to be a combination of these. For each of the decorrelation behaviors under study, the vortex trails were tracked while varying the rate of decorrelation. Parameters such as the decorrelation length, average trail length and the deviation of the vortices as they traversed in the speckle field, were studied. Empirical studies were also performed to define the distinction between trails arising from different speckle decorrelation behaviors. The initial studies under stationary speckle fields were followed up by similar studies on shifting fields. A new idea to employ Poincaŕe plots in speckle analysis has also been introduced. Our studies indicate that tracking OVs can be a potential method to study cell and tissue dynamics.

Phase-recovery improvement using analytic wavelet transform analysis of a noisy interferogram cepstrum.

PubMed

Etchepareborda, Pablo; Vadnjal, Ana Laura; Federico, Alejandro; Kaufmann, Guillermo H

2012-09-15

We evaluate the extension of the exact nonlinear reconstruction technique developed for digital holography to the phase-recovery problems presented by other optical interferometric methods, which use carrier modulation. It is shown that the introduction of an analytic wavelet analysis in the ridge of the cepstrum transformation corresponding to the analyzed interferogram can be closely related to the well-known wavelet analysis of the interferometric intensity. Subsequently, the phase-recovery process is improved. The advantages and limitations of this framework are analyzed and discussed using numerical simulations in singular scalar light fields and in temporal speckle pattern interferometry.

Improvement of Speckle Contrast Image Processing by an Efficient Algorithm.

PubMed

Steimers, A; Farnung, W; Kohl-Bareis, M

2016-01-01

We demonstrate an efficient algorithm for the temporal and spatial based calculation of speckle contrast for the imaging of blood flow by laser speckle contrast analysis (LASCA). It reduces the numerical complexity of necessary calculations, facilitates a multi-core and many-core implementation of the speckle analysis and enables an independence of temporal or spatial resolution and SNR. The new algorithm was evaluated for both spatial and temporal based analysis of speckle patterns with different image sizes and amounts of recruited pixels as sequential, multi-core and many-core code.

Bio-speckle assessment of bruising in fruits

NASA Astrophysics Data System (ADS)

Pajuelo, M.; Baldwin, G.; Rabal, H.; Cap, N.; Arizaga, R.; Trivi, M.

2003-07-01

The dynamic speckle patterns or bio-speckle is a phenomenon produced by laser illumination of active materials, such as a biological tissue. Fruits, even hard peel ones, show a speckle activity that can be related to maturity, turgor, damage, aging, and mechanical properties. In this case, we suggest a bio-speckle technique as a potential methodology for the study of impact on apples and the analysis of bruises produced by them. The aim is to correlate physical properties of apples with quality factors using a non-contact and non-invasive technique.

Robust sensor for turbidity measurement from light scattering and absorbing liquids.

PubMed

Kontturi, Ville; Turunen, Petri; Uozumi, Jun; Peiponen, Kai-Erik

2009-12-01

Internationally standardized turbidity measurements for probing solid particles in liquid are problematic in the case of simultaneous light scattering and absorption. A method and a sensor to determine the turbidity in the presence of light absorption are presented. The developed sensor makes use of the total internal reflection of a laser beam at the liquid-prism interface, and the turbidity is assessed using the concept of laser speckle pattern. Using average filtering in speckle data analyzing the observed dynamic speckle pattern, which is due to light scattering from particles and the static speckle due to stray light of the sensor, can be separated from each other. Good correlation between the standard deviation of dynamic speckle and turbidity value for nonabsorbing and for absorbing liquids was observed. The sensor is suggested, for instance, for the measurement of ill-behaved as well as small-volume turbid liquids in both medicine and process industry.

Digital reconstruction of Young's fringes using Fresnel transformation

NASA Astrophysics Data System (ADS)

Kulenovic, Rudi; Song, Yaozu; Renninger, P.; Groll, Manfred

1997-11-01

This paper deals with the digital numerical reconstruction of Young's fringes from laser speckle photography by means of the Fresnel-transformation. The physical model of the optical reconstruction of a specklegram is a near-field Fresnel-diffraction phenomenon which can be mathematically described by the Fresnel-transformation. Therefore, the interference phenomena can be directly calculated by a microcomputer.If additional a CCD-camera is used for specklegram recording the measurement procedure and evaluation process can be completely carried out in a digital way. Compared with conventional laser speckle photography no holographic plates, no wet development process and no optical specklegram reconstruction are needed. These advantages reveal a wide future in scientific and engineering applications. The basic principle of the numerical reconstruction is described, the effects of experimental parameters of Young's fringes are analyzed and representative results are presented.

Monitoring of bread cooling by statistical analysis of laser speckle patterns

NASA Astrophysics Data System (ADS)

Lyubenova, Tanya; Stoykova, Elena; Nacheva, Elena; Ivanov, Branimir; Panchev, Ivan; Sainov, Ventseslav

2013-03-01

The phenomenon of laser speckle can be used for detection and visualization of physical or biological activity in various objects (e.g. fruits, seeds, coatings) through statistical description of speckle dynamics. The paper presents the results of non-destructive monitoring of bread cooling by co-occurrence matrix and temporal structure function analysis of speckle patterns which have been recorded continuously within a few days. In total, 72960 and 39680 images were recorded and processed for two similar bread samples respectively. The experiments proved the expected steep decrease of activity related to the processes in the bread samples during the first several hours and revealed its oscillating character within the next few days. Characterization of activity over the bread sample surface was also obtained.

Robust phase recovery in temporal speckle pattern interferometry using a 3D directional wavelet transform.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2009-08-01

We propose an approach based on a 3D directional wavelet transform to retrieve optical phase distributions in temporal speckle pattern interferometry. We show that this approach can effectively recover phase distributions in time series of speckle interferograms that are affected by sets of adjacent nonmodulated pixels. The performance of this phase retrieval approach is analyzed by introducing a temporal carrier in the out-of-plane interferometer setup and assuming modulation loss and noise effects. The advantages and limitations of this approach are finally discussed.

Optically phase-locked electronic speckle pattern interferometer system performance for vibration measurement in random displacement fields

NASA Astrophysics Data System (ADS)

Moran, Steve E.; Lugannani, Robert; Craig, Peter N.; Law, Robert L.

1989-02-01

An analysis is made of the performance of an optically phase-locked electronic speckle pattern interferometer in the presence of random noise displacements. Expressions for the phase-locked speckle contrast for single-frame imagery and the composite rms exposure for two sequentially subtracted frames are obtained in terms of the phase-locked composite and single-frame fringe functions. The noise fringe functions are evaluated for stationary, coherence-separable noise displacements obeying Gauss-Markov temporal statistics. The theoretical findings presented here are qualitatively supported by experimental results.

Photon statistics and speckle visibility spectroscopy with partially coherent X-rays.

PubMed

Li, Luxi; Kwaśniewski, Paweł; Orsi, Davide; Wiegart, Lutz; Cristofolini, Luigi; Caronna, Chiara; Fluerasu, Andrei

2014-11-01

A new approach is proposed for measuring structural dynamics in materials from multi-speckle scattering patterns obtained with partially coherent X-rays. Coherent X-ray scattering is already widely used at high-brightness synchrotron lightsources to measure dynamics using X-ray photon correlation spectroscopy, but in many situations this experimental approach based on recording long series of images (i.e. movies) is either not adequate or not practical. Following the development of visible-light speckle visibility spectroscopy, the dynamic information is obtained instead by analyzing the photon statistics and calculating the speckle contrast in single scattering patterns. This quantity, also referred to as the speckle visibility, is determined by the properties of the partially coherent beam and other experimental parameters, as well as the internal motions in the sample (dynamics). As a case study, Brownian dynamics in a low-density colloidal suspension is measured and an excellent agreement is found between correlation functions measured by X-ray photon correlation spectroscopy and the decay in speckle visibility with integration time obtained from the analysis presented here.

A Simulation Analysis of an Extension of One-Dimensional Speckle Correlation Method for Detection of General In-Plane Translation

PubMed Central

Hrabovský, Miroslav

2014-01-01

The purpose of the study is to show a proposal of an extension of a one-dimensional speckle correlation method, which is primarily intended for determination of one-dimensional object's translation, for detection of general in-plane object's translation. In that view, a numerical simulation of a displacement of the speckle field as a consequence of general in-plane object's translation is presented. The translation components a x and a y representing the projections of a vector a of the object's displacement onto both x- and y-axes in the object plane (x, y) are evaluated separately by means of the extended one-dimensional speckle correlation method. Moreover, one can perform a distinct optimization of the method by reduction of intensity values representing detected speckle patterns. The theoretical relations between the translation components a x and a y of the object and the displacement of the speckle pattern for selected geometrical arrangement are mentioned and used for the testifying of the proposed method's rightness. PMID:24592180

Tracking moving targets behind a scattering medium via speckle correlation.

PubMed

Guo, Chengfei; Liu, Jietao; Wu, Tengfei; Zhu, Lei; Shao, Xiaopeng

2018-02-01

Tracking moving targets behind a scattering medium is a challenge, and it has many important applications in various fields. Owing to the multiple scattering, instead of the object image, only a random speckle pattern can be received on the camera when light is passing through highly scattering layers. Significantly, an important feature of a speckle pattern has been found, and it showed the target information can be derived from the speckle correlation. In this work, inspired by the notions used in computer vision and deformation detection, by specific simulations and experiments, we demonstrate a simple object tracking method, in which by using the speckle correlation, the movement of a hidden object can be tracked in the lateral direction and axial direction. In addition, the rotation state of the moving target can also be recognized by utilizing the autocorrelation of a speckle. This work will be beneficial for biomedical applications in the fields of quantitative analysis of the working mechanisms of a micro-object and the acquisition of dynamical information of the micro-object motion.

Tinea nigra Presenting Speckled or “Salt and Pepper” Pattern

PubMed Central

Rossetto, André Luiz; Cruz, Rosana Cé Bella; Junior, Vidal Haddad

2014-01-01

A 7-year-old Caucasian female resident of the southern coast of Brazil presented dark spots on the left palm that converged to a unique macule with speckled pattern at about 1 month. The mycological exam and the fungi culture were typical of Hortaea werneckii, the agent of the superficial mycosis Tinea nigra. The patient received butenafine hydrochloride 1% for 30 days, resulting in a complete remission of the lesion. At a follow-up visit 12 months after treatment, there was no lesion recurrence. We describe a form of rare geographical Tinea nigra with a speckled pattern. The “salt and pepper” aspect should be taken into consideration when the mycosis was suspected. PMID:24898980

Second-order oriented partial-differential equations for denoising in electronic-speckle-pattern interferometry fringes.

PubMed

Tang, Chen; Han, Lin; Ren, Hongwei; Zhou, Dongjian; Chang, Yiming; Wang, Xiaohang; Cui, Xiaolong

2008-10-01

We derive the second-order oriented partial-differential equations (PDEs) for denoising in electronic-speckle-pattern interferometry fringe patterns from two points of view. The first is based on variational methods, and the second is based on controlling diffusion direction. Our oriented PDE models make the diffusion along only the fringe orientation. The main advantage of our filtering method, based on oriented PDE models, is that it is very easy to implement compared with the published filtering methods along the fringe orientation. We demonstrate the performance of our oriented PDE models via application to two computer-simulated and experimentally obtained speckle fringes and compare with related PDE models.

Using grey intensity adjustment strategy to enhance the measurement accuracy of digital image correlation considering the effect of intensity saturation

NASA Astrophysics Data System (ADS)

Li, Bang-Jian; Wang, Quan-Bao; Duan, Deng-Ping; Chen, Ji-An

2018-05-01

Intensity saturation can cause decorrelation phenomenon and decrease the measurement accuracy in digital image correlation (DIC). In the paper, the grey intensity adjustment strategy is proposed to improve the measurement accuracy of DIC considering the effect of intensity saturation. First, the grey intensity adjustment strategy is described in detail, which can recover the truncated grey intensities of the saturated pixels and reduce the decorrelation phenomenon. The simulated speckle patterns are then employed to demonstrate the efficacy of the proposed strategy, which indicates that the displacement accuracy can be improved by about 40% by the proposed strategy. Finally, the true experimental image is used to show the feasibility of the proposed strategy, which indicates that the displacement accuracy can be increased by about 10% by the proposed strategy.

Development of CASI (Computer Aided Speckle Interferometry) and LSS (Laser Speckle Sensor) with Application to Material Response under High Strain Rate, High Temperature, Vibration and Fatigue

DTIC Science & Technology

1991-02-10

25. 1"S. revised mcp recerved Apnil 10, 19W, thnnsmte into a VAX- 11� computer. Fringf_ spacing * acceped for puablI Al 126. 1990 This paPetis a...W.Il.Peters, W.F.llanson mtid S.WI.-MN1il, "Detcrintation of dis. placements using an improved (digit al rorrela Ii’’i ii’’ h. . 111 aad Vision Y*omput ing...experiimental mcchaniics", Experiment al Mechianics, Vol.25, No.3, pp 232-.1-1 (1985). 5.~ A.Rosenfeld and A.C.Kak, Digital Pict nre processing, Vol 1

Dynamic Speckle Imaging with Low-Cost Devices

ERIC Educational Resources Information Center

Vannoni, Maurizio; Trivi, Marcelo; Arizaga, Ricardo; Rabal, Hector; Molesini, Giuseppe

2008-01-01

Light from a rough sample surface illuminated with a laser consists of a speckle pattern. If the surface evolves with time, the pattern becomes dynamic, following the activity of the sample. This phenomenon is used both in research and in industry to monitor processes and systems that change with time. The measuring equipment generally includes…

Assessing White Wine Viscosity Variation Using Polarized Laser Speckle: A Promising Alternative to Wine Sensory Analysis

PubMed Central

Abou Nader, Christelle; Loutfi, Hadi; Pellen, Fabrice; Le Jeune, Bernard; Le Brun, Guy; Lteif, Roger; Abboud, Marie

2017-01-01

In this paper, we report measurements of wine viscosity, correlated to polarized laser speckle results. Experiments were performed on white wine samples produced with a single grape variety. Effects of the wine making cellar, the grape variety, and the vintage on wine Brix degree, alcohol content, viscosity, and speckle parameters are considered. We show that speckle parameters, namely, spatial contrast and speckle decorrelation time, as well as the inertia moment extracted from the temporal history speckle pattern, are mainly affected by the alcohol and sugar content and hence the wine viscosity. Principal component analysis revealed a high correlation between laser speckle results on the one hand and viscosity and Brix degree values on the other. As speckle analysis proved to be an efficient method of measuring the variation of the viscosity of white mono-variety wine, one can therefore consider it as an alternative method to wine sensory analysis. PMID:29027936

Assessing White Wine Viscosity Variation Using Polarized Laser Speckle: A Promising Alternative to Wine Sensory Analysis.

PubMed

Nader, Christelle Abou; Loutfi, Hadi; Pellen, Fabrice; Jeune, Bernard Le; Le Brun, Guy; Lteif, Roger; Abboud, Marie

2017-10-13

In this paper, we report measurements of wine viscosity, correlated to polarized laser speckle results. Experiments were performed on white wine samples produced with a single grape variety. Effects of the wine making cellar, the grape variety, and the vintage on wine Brix degree, alcohol content, viscosity, and speckle parameters are considered. We show that speckle parameters, namely, spatial contrast and speckle decorrelation time, as well as the inertia moment extracted from the temporal history speckle pattern, are mainly affected by the alcohol and sugar content and hence the wine viscosity. Principal component analysis revealed a high correlation between laser speckle results on the one hand and viscosity and Brix degree values on the other. As speckle analysis proved to be an efficient method of measuring the variation of the viscosity of white mono-variety wine, one can therefore consider it as an alternative method to wine sensory analysis.

Accelerated numerical processing of electronically recorded holograms with reduced speckle noise.

PubMed

Trujillo, Carlos; Garcia-Sucerquia, Jorge

2013-09-01

The numerical reconstruction of digitally recorded holograms suffers from speckle noise. An accelerated method that uses general-purpose computing in graphics processing units to reduce that noise is shown. The proposed methodology utilizes parallelized algorithms to record, reconstruct, and superimpose multiple uncorrelated holograms of a static scene. For the best tradeoff between reduction of the speckle noise and processing time, the method records, reconstructs, and superimposes six holograms of 1024 × 1024 pixels in 68 ms; for this case, the methodology reduces the speckle noise by 58% compared with that exhibited by a single hologram. The fully parallelized method running on a commodity graphics processing unit is one order of magnitude faster than the same technique implemented on a regular CPU using its multithreading capabilities. Experimental results are shown to validate the proposal.

Numerical considerations on control of motion of nanoparticles using scattering field of laser light

NASA Astrophysics Data System (ADS)

Yokoi, Naomichi; Aizu, Yoshihisa

2017-05-01

Most of optical manipulation techniques proposed so far depend on carefully fabricated setups and samples. Similar conditions can be fixed in laboratories; however, it is still challenging to manipulate nanoparticles when the environment is not well controlled and is unknown in advance. Nonetheless, coherent light scattered by rough object generates a speckle pattern which consists of random interference speckle grains with well-defined statistical properties. In the present study, we numerically investigate the motion of a Brownian particle suspended in water under the illumination of a speckle pattern. Particle-captured time and size of particle-captured area are quantitatively estimated in relation to an optical force and a speckle diameter to confirm the feasibility of the present method for performing optical manipulation tasks such as trapping and guiding.

Denoising by coupled partial differential equations and extracting phase by backpropagation neural networks for electronic speckle pattern interferometry.

PubMed

Tang, Chen; Lu, Wenjing; Chen, Song; Zhang, Zhen; Li, Botao; Wang, Wenping; Han, Lin

2007-10-20

We extend and refine previous work [Appl. Opt. 46, 2907 (2007)]. Combining the coupled nonlinear partial differential equations (PDEs) denoising model with the ordinary differential equations enhancement method, we propose the new denoising and enhancing model for electronic speckle pattern interferometry (ESPI) fringe patterns. Meanwhile, we propose the backpropagation neural networks (BPNN) method to obtain unwrapped phase values based on a skeleton map instead of traditional interpolations. We test the introduced methods on the computer-simulated speckle ESPI fringe patterns and experimentally obtained fringe pattern, respectively. The experimental results show that the coupled nonlinear PDEs denoising model is capable of effectively removing noise, and the unwrapped phase values obtained by the BPNN method are much more accurate than those obtained by the well-known traditional interpolation. In addition, the accuracy of the BPNN method is adjustable by changing the parameters of networks such as the number of neurons.

Lensless Photoluminescence Hyperspectral Camera Employing Random Speckle Patterns.

PubMed

Žídek, Karel; Denk, Ondřej; Hlubuček, Jiří

2017-11-10

We propose and demonstrate a spectrally-resolved photoluminescence imaging setup based on the so-called single pixel camera - a technique of compressive sensing, which enables imaging by using a single-pixel photodetector. The method relies on encoding an image by a series of random patterns. In our approach, the image encoding was maintained via laser speckle patterns generated by an excitation laser beam scattered on a diffusor. By using a spectrometer as the single-pixel detector we attained a realization of a spectrally-resolved photoluminescence camera with unmatched simplicity. We present reconstructed hyperspectral images of several model scenes. We also discuss parameters affecting the imaging quality, such as the correlation degree of speckle patterns, pattern fineness, and number of datapoints. Finally, we compare the presented technique to hyperspectral imaging using sample scanning. The presented method enables photoluminescence imaging for a broad range of coherent excitation sources and detection spectral areas.

Deviations from Rayleigh statistics in ultrasonic speckle.

PubMed

Tuthill, T A; Sperry, R H; Parker, K J

1988-04-01

The statistics of speckle patterns in ultrasound images have potential for tissue characterization. In "fully developed speckle" from many random scatterers, the amplitude is widely recognized as possessing a Rayleigh distribution. This study examines how scattering populations and signal processing can produce non-Rayleigh distributions. The first order speckle statistics are shown to depend on random scatterer density and the amplitude and spacing of added periodic scatterers. Envelope detection, amplifier compression, and signal bandwidth are also shown to cause distinct changes in the signal distribution.

Coherent noise reduction in digital holographic microscopy by averaging multiple holograms recorded with a multimode laser.

PubMed

Pan, Feng; Yang, Lizhi; Xiao, Wen

2017-09-04

In digital holographic microscopy (DHM), it is undesirable to observe coherent noise in the reconstructed images. The sources of the noise are mainly the parasitic interference fringes caused by multiple reflections and the speckle pattern caused by the optical scattering on the object surface. Here we propose a noise reduction approach in DHM by averaging multiple holograms recorded with a multimode laser. Based on the periodicity of the temporal coherence of a multimode semiconductor laser, we acquire a series of holograms by changing the optical path length difference between the reference beam and object beam. Because of the use of low coherence light, we can remove the parasitic interference fringes caused by multiple reflections in the holograms. In addition, the coherent noise patterns change in this process due to the different optical paths. Therefore, the coherent noise can be reduced by averaging the multiple reconstructions with uncorrelated noise patterns. Several experiments have been carried out to validate the effectiveness of the proposed approach for coherent noise reduction in DHM. It is shown a remarkable improvement both in amplitude imaging quality and phase measurement accuracy.

Polarization speckle imaging as a potential technique for in vivo skin cancer detection.

PubMed

Tchvialeva, Lioudmila; Dhadwal, Gurbir; Lui, Harvey; Kalia, Sunil; Zeng, Haishan; McLean, David I; Lee, Tim K

2013-06-01

Skin cancer is the most common cancer in the Western world. In order to accurately detect the disease, especially malignant melanoma-the most fatal form of skin cancer-at an early stage when the prognosis is excellent, there is an urgent need to develop noninvasive early detection methods. We believe that polarization speckle patterns, defined as a spatial distribution of depolarization ratio of traditional speckle patterns, can be an important tool for skin cancer detection. To demonstrate our technique, we conduct a large in vivo clinical study of 214 skin lesions, and show that statistical moments of the polarization speckle pattern could differentiate different types of skin lesions, including three common types of skin cancers, malignant melanoma, squamous cell carcinoma, basal cell carcinoma, and two benign lesions, melanocytic nevus and seborrheic keratoses. In particular, the fourth order moment achieves better or similar sensitivity and specificity than many well-known and accepted optical techniques used to differentiate melanoma and seborrheic keratosis.

Harnessing speckle for a sub-femtometre resolved broadband wavemeter and laser stabilization

PubMed Central

Metzger, Nikolaus Klaus; Spesyvtsev, Roman; Bruce, Graham D.; Miller, Bill; Maker, Gareth T.; Malcolm, Graeme; Mazilu, Michael; Dholakia, Kishan

2017-01-01

The accurate determination and control of the wavelength of light is fundamental to many fields of science. Speckle patterns resulting from the interference of multiple reflections in disordered media are well-known to scramble the information content of light by complex but linear processes. However, these patterns are, in fact, exceptionally rich in information about the illuminating source. We use a fibre-coupled integrating sphere to generate wavelength-dependent speckle patterns, in combination with algorithms based on the transmission matrix method and principal component analysis, to realize a broadband and sensitive wavemeter. We demonstrate sub-femtometre wavelength resolution at a centre wavelength of 780 nm, and a broad calibrated measurement range from 488 to 1,064 nm. This compares favourably to the performance of conventional wavemeters. Using this speckle wavemeter as part of a feedback loop, we stabilize a 780 nm diode laser to achieve a linewidth better than 1 MHz. PMID:28580938

Polarization speckle imaging as a potential technique for in vivo skin cancer detection

NASA Astrophysics Data System (ADS)

Tchvialeva, Lioudmila; Dhadwal, Gurbir; Lui, Harvey; Kalia, Sunil; Zeng, Haishan; McLean, David I.; Lee, Tim K.

2013-06-01

Skin cancer is the most common cancer in the Western world. In order to accurately detect the disease, especially malignant melanoma-the most fatal form of skin cancer-at an early stage when the prognosis is excellent, there is an urgent need to develop noninvasive early detection methods. We believe that polarization speckle patterns, defined as a spatial distribution of depolarization ratio of traditional speckle patterns, can be an important tool for skin cancer detection. To demonstrate our technique, we conduct a large in vivo clinical study of 214 skin lesions, and show that statistical moments of the polarization speckle pattern could differentiate different types of skin lesions, including three common types of skin cancers, malignant melanoma, squamous cell carcinoma, basal cell carcinoma, and two benign lesions, melanocytic nevus and seborrheic keratoses. In particular, the fourth order moment achieves better or similar sensitivity and specificity than many well-known and accepted optical techniques used to differentiate melanoma and seborrheic keratosis.

Software phantom with realistic speckle modeling for validation of image analysis methods in echocardiography

NASA Astrophysics Data System (ADS)

Law, Yuen C.; Tenbrinck, Daniel; Jiang, Xiaoyi; Kuhlen, Torsten

2014-03-01

Computer-assisted processing and interpretation of medical ultrasound images is one of the most challenging tasks within image analysis. Physical phenomena in ultrasonographic images, e.g., the characteristic speckle noise and shadowing effects, make the majority of standard methods from image analysis non optimal. Furthermore, validation of adapted computer vision methods proves to be difficult due to missing ground truth information. There is no widely accepted software phantom in the community and existing software phantoms are not exible enough to support the use of specific speckle models for different tissue types, e.g., muscle and fat tissue. In this work we propose an anatomical software phantom with a realistic speckle pattern simulation to _ll this gap and provide a exible tool for validation purposes in medical ultrasound image analysis. We discuss the generation of speckle patterns and perform statistical analysis of the simulated textures to obtain quantitative measures of the realism and accuracy regarding the resulting textures.

Airplane wing deformation and flight flutter detection method by using three-dimensional speckle image correlation technology.

PubMed

Wu, Jun; Yu, Zhijing; Wang, Tao; Zhuge, Jingchang; Ji, Yue; Xue, Bin

2017-06-01

Airplane wing deformation is an important element of aerodynamic characteristics, structure design, and fatigue analysis for aircraft manufacturing, as well as a main test content of certification regarding flutter for airplanes. This paper presents a novel real-time detection method for wing deformation and flight flutter detection by using three-dimensional speckle image correlation technology. Speckle patterns whose positions are determined through the vibration characteristic of the aircraft are coated on the wing; then the speckle patterns are imaged by CCD cameras which are mounted inside the aircraft cabin. In order to reduce the computation, a matching technique based on Geodetic Systems Incorporated coded points combined with the classical epipolar constraint is proposed, and a displacement vector map for the aircraft wing can be obtained through comparing the coordinates of speckle points before and after deformation. Finally, verification experiments containing static and dynamic tests by using an aircraft wing model demonstrate the accuracy and effectiveness of the proposed method.

Tinea nigra presenting speckled or "salt and pepper" pattern.

PubMed

Rossetto, André Luiz; Cruz, Rosana Cé Bella; Haddad, Vidal Junior

2014-06-01

A 7-year-old Caucasian female resident of the southern coast of Brazil presented dark spots on the left palm that converged to a unique macule with speckled pattern at about 1 month. The mycological exam and the fungi culture were typical of Hortaea werneckii, the agent of the superficial mycosis Tinea nigra. The patient received butenafine hydrochloride 1% for 30 days, resulting in a complete remission of the lesion. At a follow-up visit 12 months after treatment, there was no lesion recurrence. We describe a form of rare geographical Tinea nigra with a speckled pattern. The "salt and pepper" aspect should be taken into consideration when the mycosis was suspected. © The American Society of Tropical Medicine and Hygiene.

Spatial phase-shift dual-beam speckle interferometry.

PubMed

Gao, Xinya; Yang, Lianxiang; Wang, Yonghong; Zhang, Boyang; Dan, Xizuo; Li, Junrui; Wu, Sijin

2018-01-20

The spatial phase-shift technique has been successfully applied to an out-of-plane speckle interferometry system. Its application to a pure in-plane sensitive system has not been reported yet. This paper presents a novel optical configuration that enables the application of the spatial phase-shift technique to pure in-plane sensitive dual-beam speckle interferometry. The new spatial phase-shift dual-beam speckle interferometry (SPS-DBSP) uses a dual-beam in-plane electronic speckle pattern interferometry configuration with individual aperture shears, avoiding the interference in the object plane by the use of a low-coherence source, and different optical paths. The measured object is illuminated by two incoherent beams that are generated by a delay line, which is larger than the coherence length of the laser. The two beams reflected from the object surface interfere with each other at the CCD plane because of different optical paths. A spatial phase shift is introduced by the angle between the two apertures when they are mapped to the same optical axis. The phase of the in-plane deformation can directly be extracted from the speckle patterns by the Fourier transform method. The capability of SPS-DBSI is demonstrated by theoretical discussion as well as experiments.

Method for measuring residual stresses in materials by plastically deforming the material and interference pattern comparison

DOEpatents

Pechersky, Martin J.

1995-01-01

A method for measuring residual stress in a material comprising the steps of establishing a speckle pattern on the surface with a first laser then heating a portion of that pattern with an infrared laser until the surface plastically deforms. Comparing the speckle patterns before and after deformation by subtracting one pattern from the other will produce a fringe pattern that serves as a visual and quantitative indication of the degree to which the plasticized surface responded to the stress dung heating and enables calculation of the stress.

Camera system resolution and its influence on digital image correlation

DOE PAGES

Reu, Phillip L.; Sweatt, William; Miller, Timothy; ...

2014-09-21

Digital image correlation (DIC) uses images from a camera and lens system to make quantitative measurements of the shape, displacement, and strain of test objects. This increasingly popular method has had little research on the influence of the imaging system resolution on the DIC results. This paper investigates the entire imaging system and studies how both the camera and lens resolution influence the DIC results as a function of the system Modulation Transfer Function (MTF). It will show that when making spatial resolution decisions (including speckle size) the resolution limiting component should be considered. A consequence of the loss ofmore » spatial resolution is that the DIC uncertainties will be increased. This is demonstrated using both synthetic and experimental images with varying resolution. The loss of image resolution and DIC accuracy can be compensated for by increasing the subset size, or better, by increasing the speckle size. The speckle-size and spatial resolution are now a function of the lens resolution rather than the more typical assumption of the pixel size. The study will demonstrate the tradeoffs associated with limited lens resolution.« less

Speckle-modulating optical coherence tomography in living mice and humans.

PubMed

Liba, Orly; Lew, Matthew D; SoRelle, Elliott D; Dutta, Rebecca; Sen, Debasish; Moshfeghi, Darius M; Chu, Steven; de la Zerda, Adam

2017-06-20

Optical coherence tomography (OCT) is a powerful biomedical imaging technology that relies on the coherent detection of backscattered light to image tissue morphology in vivo. As a consequence, OCT is susceptible to coherent noise (speckle noise), which imposes significant limitations on its diagnostic capabilities. Here we show speckle-modulating OCT (SM-OCT), a method based purely on light manipulation that virtually eliminates speckle noise originating from a sample. SM-OCT accomplishes this by creating and averaging an unlimited number of scans with uncorrelated speckle patterns without compromising spatial resolution. Using SM-OCT, we reveal small structures in the tissues of living animals, such as the inner stromal structure of a live mouse cornea, the fine structures inside the mouse pinna, and sweat ducts and Meissner's corpuscle in the human fingertip skin-features that are otherwise obscured by speckle noise when using conventional OCT or OCT with current state of the art speckle reduction methods.

Speckle-modulating optical coherence tomography in living mice and humans

PubMed Central

Liba, Orly; Lew, Matthew D.; SoRelle, Elliott D.; Dutta, Rebecca; Sen, Debasish; Moshfeghi, Darius M.; Chu, Steven; de la Zerda, Adam

2017-01-01

Optical coherence tomography (OCT) is a powerful biomedical imaging technology that relies on the coherent detection of backscattered light to image tissue morphology in vivo. As a consequence, OCT is susceptible to coherent noise (speckle noise), which imposes significant limitations on its diagnostic capabilities. Here we show speckle-modulating OCT (SM-OCT), a method based purely on light manipulation that virtually eliminates speckle noise originating from a sample. SM-OCT accomplishes this by creating and averaging an unlimited number of scans with uncorrelated speckle patterns without compromising spatial resolution. Using SM-OCT, we reveal small structures in the tissues of living animals, such as the inner stromal structure of a live mouse cornea, the fine structures inside the mouse pinna, and sweat ducts and Meissner’s corpuscle in the human fingertip skin—features that are otherwise obscured by speckle noise when using conventional OCT or OCT with current state of the art speckle reduction methods. PMID:28632205

Speckle-modulating optical coherence tomography in living mice and humans

NASA Astrophysics Data System (ADS)

Liba, Orly; Lew, Matthew D.; Sorelle, Elliott D.; Dutta, Rebecca; Sen, Debasish; Moshfeghi, Darius M.; Chu, Steven; de La Zerda, Adam

2017-06-01

Optical coherence tomography (OCT) is a powerful biomedical imaging technology that relies on the coherent detection of backscattered light to image tissue morphology in vivo. As a consequence, OCT is susceptible to coherent noise (speckle noise), which imposes significant limitations on its diagnostic capabilities. Here we show speckle-modulating OCT (SM-OCT), a method based purely on light manipulation that virtually eliminates speckle noise originating from a sample. SM-OCT accomplishes this by creating and averaging an unlimited number of scans with uncorrelated speckle patterns without compromising spatial resolution. Using SM-OCT, we reveal small structures in the tissues of living animals, such as the inner stromal structure of a live mouse cornea, the fine structures inside the mouse pinna, and sweat ducts and Meissner's corpuscle in the human fingertip skin--features that are otherwise obscured by speckle noise when using conventional OCT or OCT with current state of the art speckle reduction methods.

A review of state-of-the-art speckle reduction techniques for optical coherence tomography fingertip scans

NASA Astrophysics Data System (ADS)

Darlow, Luke N.; Akhoury, Sharat S.; Connan, James

2015-02-01

Standard surface fingerprint scanners are vulnerable to counterfeiting attacks and also failure due to skin damage and distortion. Thus a high security and damage resistant means of fingerprint acquisition is needed, providing scope for new approaches and technologies. Optical Coherence Tomography (OCT) is a high resolution imaging technology that can be used to image the human fingertip and allow for the extraction of a subsurface fingerprint. Being robust toward spoofing and damage, the subsurface fingerprint is an attractive solution. However, the nature of the OCT scanning process induces speckle: a correlative and multiplicative noise. Six speckle reducing filters for the digital enhancement of OCT fingertip scans have been evaluated. The optimized Bayesian non-local means algorithm improved the structural similarity between processed and reference images by 34%, increased the signal-to-noise ratio, and yielded the most promising visual results. An adaptive wavelet approach, originally designed for ultrasound imaging, and a speckle reducing anisotropic diffusion approach also yielded promising results. A reformulation of these in future work, with an OCT-speckle specific model, may improve their performance.

Digital off-axis holographic interferometry with simulated wavefront.

PubMed

Belashov, A V; Petrov, N V; Semenova, I V

2014-11-17

The paper presents a novel algorithm based on digital holographic interferometry and being promising for evaluation of phase variations from highly noisy or modulated by speckle-structures digital holograms. The suggested algorithm simulates an interferogram in finite width fringes, by analogy with classical double exposure holographic interferometry. Thus obtained interferogram is then processed as a digital hologram. The advantages of the suggested approach are demonstrated in numerical experiments on calculations of differences in phase distributions of wave fronts modulated by speckle structure, as well as in a physical experiment on the analysis of laser-induced heating dynamics of an aqueous solution of a photosensitizer. It is shown that owing to the inherent capability of the approach to perform adjustable smoothing of compared wave fronts, the resulting difference undergoes noise filtering. This capability of adjustable smoothing may be used to minimize losses in spatial resolution. Since the method allows to vary an observation angle of compared wave fields, an opportunity to compensate misalignment of optical axes of these wave fronts arises. This feature can be required, for example, when using two different setups in comparative digital holography or for compensation of recording system displacements during a set of exposures in studies of dynamic processes.

Calibration of a speckle-based compressive sensing receiver

NASA Astrophysics Data System (ADS)

Sefler, George A.; Shaw, T. Justin; Stapleton, Andrew D.; Valley, George C.

2017-02-01

Optical speckle in a multimode waveguide has been proposed to perform the function of a compressive sensing (CS) measurement matrix (MM) in a receiver for GHz-band radio frequency (RF) signals. Unlike other devices used for the CS MM, e.g. the digital micromirror device (DMD) used in the single pixel camera, the elements of the speckle MM are not known before use and must be measured and calibrated. In our system, the RF signal is modulated on a repetitively pulsed chirped wavelength laser source, generated from mode-locked laser pulses that have been dispersed in time or from an electrically addressed distributed Bragg reflector laser. Next, the optical beam with RF propagates through a multimode fiber or waveguide, which applies different weights in wavelength (or equivalently time) and space and performs the function of the CS MM. The output of the guide is directed to or imaged on a bank of photodiodes with integration time set to the pulse length of the chirp waveform. The output of each photodiode is digitized by an analog-to-digital converter (ADC), and the data from these ADCs are used to form the CS measurement vector. Accurate recovery of the RF signal from CS measurements depends critically on knowledge of the weights in the MM. Here we present results using a stable wavelength laser source to probe the guide.

Pre-cataract surgery test using speckle pattern

NASA Astrophysics Data System (ADS)

Jutamulia, Suganda; Wihardjo, Erning; Widjaja, Joewono

2016-11-01

A laser diode device for pre cataract surgery test is proposed. The operation is based on the speckle generated on the retina by the cataract lens, when the cataract lens is illuminated with a coherent laser light.

Brownian Motion in a Speckle Light Field: Tunable Anomalous Diffusion and Selective Optical Manipulation

PubMed Central

Volpe, Giorgio; Volpe, Giovanni; Gigan, Sylvain

2014-01-01

The motion of particles in random potentials occurs in several natural phenomena ranging from the mobility of organelles within a biological cell to the diffusion of stars within a galaxy. A Brownian particle moving in the random optical potential associated to a speckle pattern, i.e., a complex interference pattern generated by the scattering of coherent light by a random medium, provides an ideal model system to study such phenomena. Here, we derive a theory for the motion of a Brownian particle in a speckle field and, in particular, we identify its universal characteristic timescale. Based on this theoretical insight, we show how speckle light fields can be used to control the anomalous diffusion of a Brownian particle and to perform some basic optical manipulation tasks such as guiding and sorting. Our results might broaden the perspectives of optical manipulation for real-life applications. PMID:24496461

Optical Processing of Speckle Images with Bacteriorhodopsin for Pattern Recognition

NASA Technical Reports Server (NTRS)

Downie, John D.; Tucker, Deanne (Technical Monitor)

1994-01-01

Logarithmic processing of images with multiplicative noise characteristics can be utilized to transform the image into one with an additive noise distribution. This simplifies subsequent image processing steps for applications such as image restoration or correlation for pattern recognition. One particularly common form of multiplicative noise is speckle, for which the logarithmic operation not only produces additive noise, but also makes it of constant variance (signal-independent). We examine the optical transmission properties of some bacteriorhodopsin films here and find them well suited to implement such a pointwise logarithmic transformation optically in a parallel fashion. We present experimental results of the optical conversion of speckle images into transformed images with additive, signal-independent noise statistics using the real-time photochromic properties of bacteriorhodopsin. We provide an example of improved correlation performance in terms of correlation peak signal-to-noise for such a transformed speckle image.

Synchronous Stroboscopic Electronic Speckle Pattern Interferometry

NASA Astrophysics Data System (ADS)

Soares, Oliverio D. D.

1986-10-01

Electronic Speckle Pattern Interferometry (E.S.P.I) oftenly called Electronic Holography is a practical powerful technique in non-destructive testing. Practical capabilities of the technique have been improved by fringe betterment and the control of analysis in the time domain, in particular, the scanning of the vibration cycle, with introduction of: synchronized amplitude and phase modulated pulse illumination, microcomputer control, fibre optics design, and moire evaluation techniques.

Two-photon speckle illumination for super-resolution microscopy.

PubMed

Negash, Awoke; Labouesse, Simon; Chaumet, Patrick C; Belkebir, Kamal; Giovannini, Hugues; Allain, Marc; Idier, Jérôme; Sentenac, Anne

2018-06-01

We present a numerical study of a microscopy setup in which the sample is illuminated with uncontrolled speckle patterns and the two-photon excitation fluorescence is collected on a camera. We show that, using a simple deconvolution algorithm for processing the speckle low-resolution images, this wide-field imaging technique exhibits resolution significantly better than that of two-photon excitation scanning microscopy or one-photon excitation bright-field microscopy.

Two-Dimensional Laser-Speckle Surface-Strain Gauge

NASA Technical Reports Server (NTRS)

Barranger, John P.; Lant, Christian

1992-01-01

Extension of Yamaguchi's laser-speckle surface-strain-gauge method yields data on two-dimensional surface strains in times as short as fractions of second. Laser beams probe rough spot on surface of specimen before and after processing. Changes in speckle pattern of laser light reflected from spot indicative of changes in surface strains during processing. Used to monitor strains and changes in strains induced by hot-forming and subsequent cooling of steel.

Fluorescence endoscopy using fiber speckle illumination

NASA Astrophysics Data System (ADS)

Nakano, Shuhei; Katagiri, Takashi; Matsuura, Yuji

2018-02-01

An endoscopic fluorescence imaging system based on fiber speckle illumination is proposed. In this system, a multimode fiber for transmission of excitation laser light and collection of fluorescence is inserted into a conventional flexible endoscope. Since the excitation laser light has random speckle structure, one can detect fluorescence signal corresponding to the irradiation pattern if the sample contains fluorophores. The irradiation pattern can be captured by the endoscope camera when the excitation wavelength is within the sensitivity range of the camera. By performing multiple measurements while changing the irradiation pattern, a fluorescence image is reconstructed by solving a norm minimization problem. The principle of our method was experimentally demonstrated. A 2048 pixels image of quantum dots coated on a frosted glass was successfully reconstructed by 32 measurements. We also confirmed that our method can be applied on biological tissues.

A Review of Digital Image Correlation Applied to Structura Dynamics

NASA Astrophysics Data System (ADS)

Niezrecki, Christopher; Avitabile, Peter; Warren, Christopher; Pingle, Pawan; Helfrick, Mark

2010-05-01

A significant amount of interest exists in performing non-contacting, full-field surface velocity measurement. For many years traditional non-contacting surface velocity measurements have been made by using scanning Doppler laser vibrometry, shearography, pulsed laser interferometry, pulsed holography, or an electronic speckle pattern interferometer (ESPI). Three dimensional (3D) digital image correlation (DIC) methods utilize the alignment of a stereo pair of images to obtain full-field geometry data, in three dimensions. Information about the change in geometry of an object over time can be found by comparing a sequence of images and virtual strain gages (or position sensors) can be created over the entire visible surface of the object of interest. Digital imaging techniques were first developed in the 1980s but the technology has only recently been exploited in industry and research due to the advances of digital cameras and personal computers. The use of DIC for structural dynamic measurement has only very recently been investigated. Within this paper, the advantages and limits of using DIC for dynamic measurement are reviewed. Several examples of using DIC for dynamic measurement are presented on several vibrating and rotating structures.

Tunable X-ray speckle-based phase-contrast and dark-field imaging using the unified modulated pattern analysis approach

NASA Astrophysics Data System (ADS)

Zdora, M.-C.; Thibault, P.; Deyhle, H.; Vila-Comamala, J.; Rau, C.; Zanette, I.

2018-05-01

X-ray phase-contrast and dark-field imaging provides valuable, complementary information about the specimen under study. Among the multimodal X-ray imaging methods, X-ray grating interferometry and speckle-based imaging have drawn particular attention, which, however, in their common implementations incur certain limitations that can restrict their range of applications. Recently, the unified modulated pattern analysis (UMPA) approach was proposed to overcome these limitations and combine grating- and speckle-based imaging in a single approach. Here, we demonstrate the multimodal imaging capabilities of UMPA and highlight its tunable character regarding spatial resolution, signal sensitivity and scan time by using different reconstruction parameters.

Combination of oriented partial differential equation and shearlet transform for denoising in electronic speckle pattern interferometry fringe patterns.

PubMed

Xu, Wenjun; Tang, Chen; Gu, Fan; Cheng, Jiajia

2017-04-01

It is a key step to remove the massive speckle noise in electronic speckle pattern interferometry (ESPI) fringe patterns. In the spatial-domain filtering methods, oriented partial differential equations have been demonstrated to be a powerful tool. In the transform-domain filtering methods, the shearlet transform is a state-of-the-art method. In this paper, we propose a filtering method for ESPI fringe patterns denoising, which is a combination of second-order oriented partial differential equation (SOOPDE) and the shearlet transform, named SOOPDE-Shearlet. Here, the shearlet transform is introduced into the ESPI fringe patterns denoising for the first time. This combination takes advantage of the fact that the spatial-domain filtering method SOOPDE and the transform-domain filtering method shearlet transform benefit from each other. We test the proposed SOOPDE-Shearlet on five experimentally obtained ESPI fringe patterns with poor quality and compare our method with SOOPDE, shearlet transform, windowed Fourier filtering (WFF), and coherence-enhancing diffusion (CEDPDE). Among them, WFF and CEDPDE are the state-of-the-art methods for ESPI fringe patterns denoising in transform domain and spatial domain, respectively. The experimental results have demonstrated the good performance of the proposed SOOPDE-Shearlet.

3D displacement field measurement with correlation based on the micro-geometrical surface texture

NASA Astrophysics Data System (ADS)

Bubaker-Isheil, Halima; Serri, Jérôme; Fontaine, Jean-François

2011-07-01

Image correlation methods are widely used in experimental mechanics to obtain displacement field measurements. Currently, these methods are applied using digital images of the initial and deformed surfaces sprayed with black or white paint. Speckle patterns are then captured and the correlation is performed with a high degree of accuracy to an order of 0.01 pixels. In 3D, however, stereo-correlation leads to a lower degree of accuracy. Correlation techniques are based on the search for a sub-image (or pattern) displacement field. The work presented in this paper introduces a new correlation-based approach for 3D displacement field measurement that uses an additional 3D laser scanner and a CMM (Coordinate Measurement Machine). Unlike most existing methods that require the presence of markers on the observed object (such as black speckle, grids or random patterns), this approach relies solely on micro-geometrical surface textures such as waviness, roughness and aperiodic random defects. The latter are assumed to remain sufficiently small thus providing an adequate estimate of the particle displacement. The proposed approach can be used in a wide range of applications such as sheet metal forming with large strains. The method proceeds by first obtaining cloud points using the 3D laser scanner mounted on a CMM. These points are used to create 2D maps that are then correlated. In this respect, various criteria have been investigated for creating maps consisting of patterns, which facilitate the correlation procedure. Once the maps are created, the correlation between both configurations (initial and moved) is carried out using traditional methods developed for field measurements. Measurement validation was conducted using experiments in 2D and 3D with good results for rigid displacements in 2D, 3D and 2D rotations.

Comparison of Filters Dedicated to Speckle Suppression in SAR Images

NASA Astrophysics Data System (ADS)

Kupidura, P.

2016-06-01

This paper presents the results of research on the effectiveness of different filtering methods dedicated to speckle suppression in SAR images. The tests were performed on RadarSat-2 images and on an artificial image treated with simulated speckle noise. The research analysed the performance of particular filters related to the effectiveness of speckle suppression and to the ability to preserve image details and edges. Speckle is a phenomenon inherent to radar images - a deterministic noise connected with land cover type, but also causing significant changes in digital numbers of pixels. As a result, it may affect interpretation, classification and other processes concerning radar images. Speckle, resembling "salt and pepper" noise, has the form of a set of relatively small groups of pixels of values markedly different from values of other pixels representing the same type of land cover. Suppression of this noise may also cause suppression of small image details, therefore the ability to preserve the important parts of an image, was analysed as well. In the present study, selected filters were tested, and methods dedicated particularly to speckle noise suppression: Frost, Gamma-MAP, Lee, Lee-Sigma, Local Region, general filtering methods which might be effective in this respect: Mean, Median, in addition to morphological filters (alternate sequential filters with multiple structuring element and by reconstruction). The analysis presented in this paper compared the effectiveness of different filtering methods. It proved that some of the dedicated radar filters are efficient tools for speckle suppression, but also demonstrated a significant efficiency of the morphological approach, especially its ability to preserve image details.

Principal Component Analysis in the Spectral Analysis of the Dynamic Laser Speckle Patterns

NASA Astrophysics Data System (ADS)

Ribeiro, K. M.; Braga, R. A., Jr.; Horgan, G. W.; Ferreira, D. D.; Safadi, T.

2014-02-01

Dynamic laser speckle is a phenomenon that interprets an optical patterns formed by illuminating a surface under changes with coherent light. Therefore, the dynamic change of the speckle patterns caused by biological material is known as biospeckle. Usually, these patterns of optical interference evolving in time are analyzed by graphical or numerical methods, and the analysis in frequency domain has also been an option, however involving large computational requirements which demands new approaches to filter the images in time. Principal component analysis (PCA) works with the statistical decorrelation of data and it can be used as a data filtering. In this context, the present work evaluated the PCA technique to filter in time the data from the biospeckle images aiming the reduction of time computer consuming and improving the robustness of the filtering. It was used 64 images of biospeckle in time observed in a maize seed. The images were arranged in a data matrix and statistically uncorrelated by PCA technique, and the reconstructed signals were analyzed using the routine graphical and numerical methods to analyze the biospeckle. Results showed the potential of the PCA tool in filtering the dynamic laser speckle data, with the definition of markers of principal components related to the biological phenomena and with the advantage of fast computational processing.

Simultaneous displacement and slope measurement in electronic speckle pattern interferometry using adjustable aperture multiplexing.

PubMed

Lu, Min; Wang, Shengjia; Aulbach, Laura; Koch, Alexander W

2016-08-01

This paper suggests the use of adjustable aperture multiplexing (AAM), a method which is able to introduce multiple tunable carrier frequencies into a three-beam electronic speckle pattern interferometer to measure the out-of-plane displacement and its first-order derivative simultaneously. In the optical arrangement, two single apertures are located in the object and reference light paths, respectively. In cooperation with two adjustable mirrors, virtual images of the single apertures construct three pairs of virtual double apertures with variable aperture opening sizes and aperture distances. By setting the aperture parameter properly, three tunable spatial carrier frequencies are produced within the speckle pattern and completely separate the information of three interferograms in the frequency domain. By applying the inverse Fourier transform to a selected spectrum, its corresponding phase difference distribution can thus be evaluated. Therefore, we can obtain the phase map due to the deformation as well as its slope of the test surface from two speckle patterns which are recorded at different loading events. By this means, simultaneous and dynamic measurements are realized. AAM has greatly simplified the measurement system, which contributes to improving the system stability and increasing the system flexibility and adaptability to various measurement requirements. This paper presents the AAM working principle, the phase retrieval using spatial carrier frequency, and preliminary experimental results.

The Correlation of Blood Glucose Concentration and the Movement of Laser Secondary Speckle Pattern of the Artery

NASA Astrophysics Data System (ADS)

Saputra, M. A.; Prajitno, P.

2018-04-01

Blood glucose is the molecule needed for human life, it usually measured invasively (by taking blood). but that measurement is still very vulnerable. The alternative method namely the non-invasive method is very interesting. In addition, the article [1] explains the relationship between the movement of the arterial pulse with glucose concentration, therefore the research study to investigate the correlation between the blood glucose and the movement of laser speckle pattern resulted from the arterial movement will be promising as the non-invasive method for measuring the blood glucose concentration. In this study, the laser speckle pattern imaging method, where the microscopically movement of the object is illuminated by a laser beam and recorded by the high-speed camera in a certain interval time, are used to identify the movement patterns of the artery. From the image processing, the graphs such as electrocardiograph (ECG) can be extracted. The average of the maximum peaks of the graph can be correlated with the blood glucose concentration in the blood, as the same as shown in the article [2]. From the data that has been obtained in this research, the movement of the speckle tends to increase in accordance with the rise of blood glucose concentration.

Simple and versatile heterodyne whole-field interferometer for phase optics characterization.

PubMed

Silva, D M; Barbosa, E A; Wetter, N U

2012-10-01

A wavefront sensor for thermally induced lens and passive lens characterization based on low-coherence digital speckle interferometry was developed and studied. By illuminating the optical setup with two slightly detuned red diode lasers, whole-field contour interference fringes were generated according to the resulting synthetic wavelength. For fringe pattern visualization the optical setup used the light transmitted through a ground glass plate as object beam. The performance of the sensor was investigated and its versatility was demonstrated by measuring the thermal lens induced in an Er-doped glass sample pumped by a 1.76-W diode laser emitting at 976 nm and by evaluating the wavefront distortion introduced by an ophthalmic progressive lens.

Operation Program for the Spatially Phase-Shifted Digital Speckle Pattern Interferometer - SPS-DSPI

NASA Technical Reports Server (NTRS)

Blake, Peter N.; Jones, Joycelyn T.; Hostetter, Carl F.; Greenfield, Perry; Miller, Todd

2010-01-01

SPS-DSPI software has been revised so that Goddard optical engineers can operate the instrument, instead of data programmers. The user interface has been improved to view the data collected by the SPS-DSPI, with a real-time mode and a play-back mode. The SPS-DSPI has been developed by NASA/GSFC to measure the temperature distortions of the primary-mirror backplane structure for the James Webb Space Telescope. It requires a team of computer specialists to run successfully, because, at the time of this reporting, it just finished the prototype stage. This software improvement will transition the instrument to become available for use by many programs that measure distortion

Speckle: tool for diagnosis assistance

NASA Astrophysics Data System (ADS)

Carvalho, O.; Guyot, S.; Roy, L.; Benderitter, M.; Clairac, B.

2006-09-01

In this paper, we present a new approach of the speckle phenomenon. This method is based on the fractal Brownian motion theory and allows the extraction of three stochastic parameters to characterize the speckle pattern. For the first time, we present the results of this method applied to the discrimination of the healthy vs. pathologic skin. We also demonstrate, in case of the scleroderma, than this method is more accurate than the classical frequential approach.

Speckle reduction process based on digital filtering and wavelet compounding in optical coherence tomography for dermatology

NASA Astrophysics Data System (ADS)

Gómez Valverde, Juan J.; Ortuño, Juan E.; Guerra, Pedro; Hermann, Boris; Zabihian, Behrooz; Rubio-Guivernau, José L.; Santos, Andrés.; Drexler, Wolfgang; Ledesma-Carbayo, Maria J.

2015-07-01

Optical Coherence Tomography (OCT) has shown a great potential as a complementary imaging tool in the diagnosis of skin diseases. Speckle noise is the most prominent artifact present in OCT images and could limit the interpretation and detection capabilities. In this work we propose a new speckle reduction process and compare it with various denoising filters with high edge-preserving potential, using several sets of dermatological OCT B-scans. To validate the performance we used a custom-designed spectral domain OCT and two different data set groups. The first group consisted in five datasets of a single B-scan captured N times (with N<20), the second were five 3D volumes of 25 Bscans. As quality metrics we used signal to noise (SNR), contrast to noise (CNR) and equivalent number of looks (ENL) ratios. Our results show that a process based on a combination of a 2D enhanced sigma digital filter and a wavelet compounding method achieves the best results in terms of the improvement of the quality metrics. In the first group of individual B-scans we achieved improvements in SNR, CNR and ENL of 16.87 dB, 2.19 and 328 respectively; for the 3D volume datasets the improvements were 15.65 dB, 3.44 and 1148. Our results suggest that the proposed enhancement process may significantly reduce speckle, increasing SNR, CNR and ENL and reducing the number of extra acquisitions of the same frame.

Residual stress measurement of PMMA by combining drilling-hole with digital speckle correlation method

NASA Astrophysics Data System (ADS)

Yao, X. F.; Xiong, T. C.; Xu, H. M.; Wan, J. P.; Long, G. R.

2008-11-01

The residual stresses of the PMMA (polymethyl methacrylate) specimens after being drilled, reamed and polished respectively are investigated using the digital speckle correlation experimental method,. According to the displacement fields around the correlated calculated region, the polynomial curve fitting method is used to obtain the continuous displacement fields, and the strain fields can be obtained from the derivative of the displacement fields. Considering the constitutive equation of the material, the expression of the residual stress can be presented. During the data processing, according to the fitting effect of the data, the calculation region of the correlated speckles and the degree of the polynomial fitting curve is decided. These results show that the maximum stress is at the hole-wall of the drilling hole specimen and with the increasing of the diameter of the drilled hole, the residual stress resulting from the hole drilling increases, whereas the process of reaming and polishing hole can reduce the residual stress. The relative large discrete degree of the residual stress is due to the chip removal ability of the drill bit, the cutting feed of the drill and other various reasons.

Non-Gaussian Correlations between Reflected and Transmitted Intensity Patterns Emerging from Opaque Disordered Media

NASA Astrophysics Data System (ADS)

Starshynov, I.; Paniagua-Diaz, A. M.; Fayard, N.; Goetschy, A.; Pierrat, R.; Carminati, R.; Bertolotti, J.

2018-04-01

The propagation of monochromatic light through a scattering medium produces speckle patterns in reflection and transmission, and the apparent randomness of these patterns prevents direct imaging through thick turbid media. Yet, since elastic multiple scattering is fundamentally a linear and deterministic process, information is not lost but distributed among many degrees of freedom that can be resolved and manipulated. Here, we demonstrate experimentally that the reflected and transmitted speckle patterns are robustly correlated, and we unravel all the complex and unexpected features of this fundamentally non-Gaussian and long-range correlation. In particular, we show that it is preserved even for opaque media with thickness much larger than the scattering mean free path, proving that information survives the multiple scattering process and can be recovered. The existence of correlations between the two sides of a scattering medium opens up new possibilities for the control of transmitted light without any feedback from the target side, but using only information gathered from the reflected speckle.

Multimode waveguide speckle patterns for compressive sensing.

PubMed

Valley, George C; Sefler, George A; Justin Shaw, T

2016-06-01

Compressive sensing (CS) of sparse gigahertz-band RF signals using microwave photonics may achieve better performances with smaller size, weight, and power than electronic CS or conventional Nyquist rate sampling. The critical element in a CS system is the device that produces the CS measurement matrix (MM). We show that passive speckle patterns in multimode waveguides potentially provide excellent MMs for CS. We measure and calculate the MM for a multimode fiber and perform simulations using this MM in a CS system. We show that the speckle MM exhibits the sharp phase transition and coherence properties needed for CS and that these properties are similar to those of a sub-Gaussian MM with the same mean and standard deviation. We calculate the MM for a multimode planar waveguide and find dimensions of the planar guide that give a speckle MM with a performance similar to that of the multimode fiber. The CS simulations show that all measured and calculated speckle MMs exhibit a robust performance with equal amplitude signals that are sparse in time, in frequency, and in wavelets (Haar wavelet transform). The planar waveguide results indicate a path to a microwave photonic integrated circuit for measuring sparse gigahertz-band RF signals using CS.

Influence of condition of growth of bacterial colonies on fractal dimension of bacterial speckle patterns

NASA Astrophysics Data System (ADS)

Ulyanov, Alexander S.; Lyapina, Anna M.; Ulianova, Onega V.; Feodorova, Valentina A.

2010-10-01

New field of application of fractal dimensions is proposed. A technique, based on the calculation of fractal dimension, was used for express-diagnostics and identification of bacteria of the vaccine strain Yersinia pestis EV line NIIEG. Purpose of this study was the experimental investigation of properties of speckle patterns, formed under laser illumination of a single colony of the strain that was grown on different agars.

Influence of condition of growth of bacterial colonies on fractal dimension of bacterial speckle patterns

NASA Astrophysics Data System (ADS)

Ulyanov, Alexander S.; Lyapina, Anna M.; Ulianova, Onega V.; Feodorova, Valentina A.

2011-03-01

New field of application of fractal dimensions is proposed. A technique, based on the calculation of fractal dimension, was used for express-diagnostics and identification of bacteria of the vaccine strain Yersinia pestis EV line NIIEG. Purpose of this study was the experimental investigation of properties of speckle patterns, formed under laser illumination of a single colony of the strain that was grown on different agars.

Essential features of residual stress determination in thin-walled plane structures in a base of whole field interferometric measurements

NASA Astrophysics Data System (ADS)

Pisarev, Vladimir S.; Odintsev, I.; Balalov, V.; Apalkov, A.

2003-05-01

Sophisticated technique for reliable quantitative deriving residual stress values from initial experimental data, which are inherent in combined implementing the hole drilling method with both holographic and speckle interferometry, is described in detail. The approach developed includes both possible ways of obtaining initial experimental information. The first of them consists of recording a set of required interference fringe patterns, which are resulted from residual stress energy release after through hole drilling, in two orthogonal directions that coincide with principal strain directions. The second way is obtaining a series of interrelated fringe patterns when a direction of either observation in reflection hologram interferometry or dual-beam illumination in speckle interferometry lies arbitrary with respect to definite principal strain direction. A set of the most typical both actual and analogous reference fringe patterns, which are related to both reflection hologram and dual-beam speckle interferometry, are presented.

Numerical study on trapping and guiding of nanoparticles in a flow using scattering field of laser light

NASA Astrophysics Data System (ADS)

Yokoi, Naomichi; Aizu, Yoshihisa

2018-01-01

Optical trapping and guiding using laser have been proven to be useful for non-contact and non-invasive manipulation of small objects such as biological cells, organelles within cells, and dielectric particles. We have numerically investigated so far the motion of a Brownian particle suspended in still water under the illumination of a speckle pattern generated by the interference of coherent light scattered by a rough object. In the present study, we investigate numerically the motion of a particle in a water flow under the illumination of a speckle pattern that is at rest or in motion. Trajectory of the particle is simulated in relation with its size, flow velocity, maximum irradiance, and moving velocity of the speckle pattern to confirm the feasibility of the present method for performing optical trapping and guiding of the particle in the flow.

Numerical study on trapping and guiding of nanoparticles in a flow using scattering field of laser light

NASA Astrophysics Data System (ADS)

Yokoi, Naomichi; Aizu, Yoshihisa

2018-06-01

Optical trapping and guiding using laser have been proven to be useful for non-contact and non-invasive manipulation of small objects such as biological cells, organelles within cells, and dielectric particles. We have numerically investigated so far the motion of a Brownian particle suspended in still water under the illumination of a speckle pattern generated by the interference of coherent light scattered by a rough object. In the present study, we investigate numerically the motion of a particle in a water flow under the illumination of a speckle pattern that is at rest or in motion. Trajectory of the particle is simulated in relation with its size, flow velocity, maximum irradiance, and moving velocity of the speckle pattern to confirm the feasibility of the present method for performing optical trapping and guiding of the particle in the flow.

Self-marking phase-stepping electronic speckle pattern interfometry (ESPI) for 3D displacement measurement on cathode ray tube (CRT)-panels

NASA Astrophysics Data System (ADS)

Huang, M. J.; Liu, Zhao-Cheng; Jhang, Jhen-Huei

2002-11-01

This study demonstrates the feasibility of applying phase-shifting electronic speckle pattern interfometry to measure the deformation field of the front panel of a cathode ray tube, to support analysis to enhance the implosion-resistance capacity under violent collapse. Two effects, the air exhaustion and shrink band constraint effects, are comprehensively investigated. The angle of an adjustable mirror is switched, to provide three sensitivity vectors that are required in 3D-displacement measurement. A Fourier filtration is employed to remove speckle noise and establish a noise-free phase map. Inconsistent points are identified and masked to prevent any possible divergence during phase unwrapping. The results show that the accuracy of this method is satisfactory.

Laser speckle visibility acoustic spectroscopy in soft turbid media

NASA Astrophysics Data System (ADS)

Wintzenrieth, Frédéric; Cohen-Addad, Sylvie; Le Merrer, Marie; Höhler, Reinhard

2014-03-01

We image the evolution in space and time of an acoustic wave propagating along the surface of turbid soft matter by shining coherent light on the sample. The wave locally modulates the speckle interference pattern of the backscattered light and the speckle visibility[2] is recorded using a camera. We show both experimentally and theoretically how the temporal and spatial correlations in this pattern can be analyzed to obtain the acoustic wavelength and attenuation length. The technique is validated using shear waves propagating in aqueous foam.[3] It may be applied to other kinds of acoustic wave in different forms of turbid soft matter, such as biological tissues, pastes or concentrated emulsions. Now at Université Lyon 1 (ILM).

Aberration-free superresolution imaging via binary speckle pattern encoding and processing

NASA Astrophysics Data System (ADS)

Ben-Eliezer, Eyal; Marom, Emanuel

2007-04-01

We present an approach that provides superresolution beyond the classical limit as well as image restoration in the presence of aberrations; in particular, the ability to obtain superresolution while extending the depth of field (DOF) simultaneously is tested experimentally. It is based on an approach, recently proposed, shown to increase the resolution significantly for in-focus images by speckle encoding and decoding. In our approach, an object multiplied by a fine binary speckle pattern may be located anywhere along an extended DOF region. Since the exact magnification is not known in the presence of defocus aberration, the acquired low-resolution image is electronically processed via a parallel-branch decoding scheme, where in each branch the image is multiplied by the same high-resolution synchronized time-varying binary speckle but with different magnification. Finally, a hard-decision algorithm chooses the branch that provides the highest-resolution output image, thus achieving insensitivity to aberrations as well as DOF variations. Simulation as well as experimental results are presented, exhibiting significant resolution improvement factors.

Universal sensitivity of speckle intensity correlations to wavefront change in light diffusers

PubMed Central

Kim, KyungDuk; Yu, Hyeonseung; Lee, KyeoReh; Park, YongKeun

2017-01-01

Here, we present a concept based on the realization that a complex medium can be used as a simple interferometer. Changes in the wavefront of an incident coherent beam can be retrieved by analyzing changes in speckle patterns when the beam passes through a light diffuser. We demonstrate that the spatial intensity correlations of the speckle patterns are independent of the light diffusers, and are solely determined by the phase changes of an incident beam. With numerical simulations using the random matrix theory, and an experimental pressure-driven wavefront-deforming setup using a microfluidic channel, we theoretically and experimentally confirm the universal sensitivity of speckle intensity correlations, which is attributed to the conservation of optical field correlation despite multiple light scattering. This work demonstrates that a light diffuser works as a simple interferometer, and presents opportunities to retrieve phase information of optical fields with a compact scattering layer in various applications in metrology, analytical chemistry, and biomedicine. PMID:28322268

Speckle correlation resolution enhancement of wide-field fluorescence imaging (Conference Presentation)

NASA Astrophysics Data System (ADS)

Yilmaz, Hasan

2016-03-01

Structured illumination enables high-resolution fluorescence imaging of nanostructures [1]. We demonstrate a new high-resolution fluorescence imaging method that uses a scattering layer with a high-index substrate as a solid immersion lens [2]. Random scattering of coherent light enables a speckle pattern with a very fine structure that illuminates the fluorescent nanospheres on the back surface of the high-index substrate. The speckle pattern is raster-scanned over the fluorescent nanospheres using a speckle correlation effect known as the optical memory effect. A series of standard-resolution fluorescence images per each speckle pattern displacement are recorded by an electron-multiplying CCD camera using a commercial microscope objective. We have developed a new phase-retrieval algorithm to reconstruct a high-resolution, wide-field image from several standard-resolution wide-field images. We have introduced phase information of Fourier components of standard-resolution images as a new constraint in our algorithm which discards ambiguities therefore ensures convergence to a unique solution. We demonstrate two-dimensional fluorescence images of a collection of nanospheres with a deconvolved Abbe resolution of 116 nm and a field of view of 10 µm × 10 µm. Our method is robust against optical aberrations and stage drifts, therefore excellent for imaging nanostructures under ambient conditions. [1] M. G. L. Gustafsson, J. Microsc. 198, 82-87 (2000). [2] H. Yilmaz, E. G. van Putten, J. Bertolotti, A. Lagendijk, W. L. Vos, and A. P. Mosk, Optica 2, 424-429 (2015).

Evaluation of Defects inside Beryllium Foils using X-ray Computed Tomography and Shearing Interferometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sakurai, Tatsuyuki; Kohmura, Yoshiki; Takeuchi, Akihisa

2007-01-19

When beryllium is used in transmission X-ray optical elements for spatially coherent beams, speckles are usually observed in the transmission images. These speckles seem to be caused by defects either inside or on the surface of beryllium foil. We measured highly polished beryllium foil using two methods, X-ray computed tomography and X-ray shearing interferometry. The results indicate that observed speckle pattern is caused by many voids inside beryllium or inner low-density regions.

Phase recovery in temporal speckle pattern interferometry using the generalized S-transform.

PubMed

Federico, Alejandro; Kaufmann, Guillermo H

2008-04-15

We propose a novel approach based on the generalized S-transform to retrieve optical phase distributions in temporal speckle pattern interferometry. The performance of the proposed approach is compared with those given by well-known techniques based on the continuous wavelet, the Hilbert transforms, and a smoothed time-frequency distribution by analyzing interferometric data degraded by noise, nonmodulating pixels, and modulation loss. The advantages and limitations of the proposed phase retrieval approach are discussed.

Speckle in the diffraction patterns of Hendricks-Teller and icosahedral glass models

NASA Technical Reports Server (NTRS)

Garg, Anupam; Levine, Dov

1988-01-01

It is shown that the X-ray diffraction patterns from the Hendricks-Teller model for layered systems and the icosahedral glass models for the icosahedral phases show large fluctuations between nearby scattering wave vectors and from sample to sample, that are quite analogous to laser speckle. The statistics of these fluctuations are studied analytically for the first model and via computer simulations for the second. The observability of these effects is discussed briefly.

Speckle interferometry with temporal phase evaluation for measuring large-object deformation.

PubMed

Joenathan, C; Franze, B; Haible, P; Tiziani, H J

1998-05-01

We propose a new method for measuring large-object deformations byusing temporal evolution of the speckles in speckleinterferometry. The principle of the method is that by deformingthe object continuously, one obtains fluctuations in the intensity ofthe speckle. A large number of frames of the object motion arecollected to be analyzed later. The phase data for whole-objectdeformation are then retrieved by inverse Fourier transformation of afiltered spectrum obtained by Fourier transformation of thesignal. With this method one is capable of measuring deformationsof more than 100 mum, which is not possible using conventionalelectronic speckle pattern interferometry. We discuss theunderlying principle of the method and the results of theexperiments. Some nondestructive testing results are alsopresented.

Synchronized diffusive-wave spectroscopy: Principle and application to sound propagation in aqueous foams.

PubMed

Crassous, Jérôme; Chasle, Patrick; Pierre, Juliette; Saint-Jalmes, Arnaud; Dollet, Benjamin

2016-03-01

We present an experimental method to measure oscillatory strains in turbid material. The material is illuminated with a laser, and the speckle patterns are recorded. The analysis of the deformations of the optical path length shows that the speckle patterns are modulated at the strain frequency. By recording those patterns synchronously with the strain source, we are able to measure the amplitude and the phase of the strain. This method is tested in the specific case of an aqueous foam where an acoustic wave propagates. The effects of material internal dynamics and heterogeneous deformations are also discussed.

Synchronized diffusive-wave spectroscopy: Principle and application to sound propagation in aqueous foams

NASA Astrophysics Data System (ADS)

Crassous, Jérôme; Chasle, Patrick; Pierre, Juliette; Saint-Jalmes, Arnaud; Dollet, Benjamin

2016-03-01

We present an experimental method to measure oscillatory strains in turbid material. The material is illuminated with a laser, and the speckle patterns are recorded. The analysis of the deformations of the optical path length shows that the speckle patterns are modulated at the strain frequency. By recording those patterns synchronously with the strain source, we are able to measure the amplitude and the phase of the strain. This method is tested in the specific case of an aqueous foam where an acoustic wave propagates. The effects of material internal dynamics and heterogeneous deformations are also discussed.

Two-Color Laser Speckle Shift Strain Measurement System

NASA Technical Reports Server (NTRS)

Tuma, Margaret L.; Krasowski, Michael J.; Oberle, Lawrence G.; Greer, Lawrence C., III; Spina, Daniel; Barranger, John

1996-01-01

A two color laser speckle shift strain measurement system based on the technique of Yamaguchi was designed. The dual wavelength light output from an Argon Ion laser was coupled into two separate single-mode optical fibers (patchcords). The output of the patchcords is incident on the test specimen (here a structural fiber). Strain on the fiber, in one direction, is produced using an Instron 4502. Shifting interference patterns or speckle patterns will be detected at real-time rates using 2 CCD cameras with image processing performed by a hardware correlator. Strain detected in fibers with diameters from 21 microns to 143 microns is expected to be resolved to 15 mu epsilon. This system was designed to be compact and robust and does not require surface preparation of the structural fibers.

Numerical consideration on trapping and guiding of nanoparticles in a flow using scattering field of laser light

NASA Astrophysics Data System (ADS)

Yokoi, Naomichi; Aizu, Yoshihisa

2017-04-01

Optical manipulation techniques proposed so far almost depend on carefully fabricated setups and samples. Similar conditions can be fixed in laboratories, however, it is still a challenging work to manipulate nanoparticles when the environment is not well controlled and is unknown in advance. Nonetheless, coherent light scattered by rough object generates speckles which are random interference patterns with well-defined statistical properties. In the present study, we numerically investigate the motion of a particle in a flow under the illumination of a speckle pattern that is at rest or in motion. Trajectory of the particle is simulated in relation to a flow velocity and a speckle contrast to confirm the feasibility of the present method for performing optical manipulation tasks such as trapping and guiding.

Design and Validation of an Infrared Badal Optometer for Laser Speckle (IBOLS)

PubMed Central

Teel, Danielle F. W.; Copland, R. James; Jacobs, Robert J.; Wells, Thad; Neal, Daniel R.; Thibos, Larry N.

2009-01-01

Purpose To validate the design of an infrared wavefront aberrometer with a Badal optometer employing the principle of laser speckle generated by a spinning disk and infrared light. The instrument was designed for subjective meridional refraction in infrared light by human patients. Methods Validation employed a model eye with known refractive error determined with an objective infrared wavefront aberrometer. The model eye was used to produce a speckle pattern on an artificial retina with controlled amounts of ametropia introduced with auxiliary ophthalmic lenses. A human observer performed the psychophysical task of observing the speckle pattern (with the aid of a video camera sensitive to infrared radiation) formed on the artificial retina. Refraction was performed by adjusting the vergence of incident light with the Badal optometer to nullify the motion of laser speckle. Validation of the method was performed for different levels of spherical ametropia and for various configurations of an astigmatic model eye. Results Subjective measurements of meridional refractive error over the range −4D to + 4D agreed with astigmatic refractive errors predicted by the power of the model eye in the meridian of motion of the spinning disk. Conclusions Use of a Badal optometer to control laser speckle is a valid method for determining subjective refractive error at infrared wavelengths. Such an instrument will be useful for comparing objective measures of refractive error obtained for the human eye with autorefractors and wavefront aberrometers that employ infrared radiation. PMID:18772719

Dynamic laser speckle for non-destructive quality evaluation of bread

NASA Astrophysics Data System (ADS)

Stoykova, E.; Ivanov, B.; Shopova, M.; Lyubenova, T.; Panchev, I.; Sainov, V.

2010-10-01

Coherent illumination of a diffuse object yields a randomly varying interference pattern, which changes over time at any modification of the object. This phenomenon can be used for detection and visualization of physical or biological activity in various objects (e.g. fruits, seeds, coatings) through statistical description of laser speckle dynamics. The present report aims at non-destructive full-field evaluation of bread by spatial-temporal characterization of laser speckle. The main purpose of the conducted experiments was to prove the ability of the dynamic speckle method to indicate activity within the studied bread samples. In the set-up for acquisition and storage of dynamic speckle patterns an expanded beam from a DPSS laser (532 nm and 100mW) illuminated the sample through a ground glass diffuser. A CCD camera, adjusted to focus the sample, recorded regularly a sequence of images (8 bits and 780 x 582 squared pixels, sized 8.1 × 8.1 μm) at sampling frequency 0.25 Hz. A temporal structure function was calculated to evaluate activity of the bread samples in time using the full images in the sequence. In total, 7 samples of two types of bread were monitored during a chemical and physical process of bread's staling. Segmentation of images into matrixes of isometric fragments was also utilized. The results proved the potential of dynamic speckle as effective means for monitoring the process of bread staling and ability of this approach to differentiate between different types of bread.

Electronic speckle pattern interferometry using vortex beams.

PubMed

Restrepo, René; Uribe-Patarroyo, Néstor; Belenguer, Tomás

2011-12-01

We show that it is possible to perform electronic speckle pattern interferometry (ESPI) using, for the first time to our knowledge, vortex beams as the reference beam. The technique we propose is easy to implement, and the advantages obtained are, among others, environmental stability, lower processing time, and the possibility to switch between traditional ESPI and spiral ESPI. The experimental results clearly show the advantages of using the proposed technique for deformation studies of complex structures. © 2011 Optical Society of America

Laser speckle imaging in the spatial frequency domain

PubMed Central

Mazhar, Amaan; Cuccia, David J.; Rice, Tyler B.; Carp, Stefan A.; Durkin, Anthony J.; Boas, David A.; Choi, Bernard; Tromberg, Bruce J.

2011-01-01

Laser Speckle Imaging (LSI) images interference patterns produced by coherent addition of scattered laser light to map subsurface tissue perfusion. However, the effect of longer path length photons is typically unknown and poses a limitation towards absolute quantification. In this work, LSI is integrated with spatial frequency domain imaging (SFDI) to suppress multiple scattering and absorption effects. First, depth sensitive speckle contrast is shown in phantoms by separating a deep source (4 mm) from a shallow source (2 mm) of speckle contrast by using a high spatial frequency of illumination (0.24 mm−1). We develop an SFD adapted correlation diffusion model and show that with high frequency (0.24 mm−1) illumination, doubling of absorption contrast results in only a 1% change in speckle contrast versus 25% change using a planar unmodulated (0 mm−1) illumination. Similar absorption change is mimicked in vivo imaging a finger occlusion and the relative speckle contrast change from baseline is 10% at 0.26 mm−1 versus 60% at 0 mm−1 during a finger occlusion. These results underscore the importance of path length and optical properties in determining speckle contrast. They provide an integrated approach for simultaneous mapping of blood flow (speckle contrast) and oxygenation (optical properties) which can be used to inform tissue metabolism. PMID:21698018

Lagrangian speckle model and tissue-motion estimation--theory.

PubMed

Maurice, R L; Bertrand, M

1999-07-01

It is known that when a tissue is subjected to movements such as rotation, shearing, scaling, etc., changes in speckle patterns that result act as a noise source, often responsible for most of the displacement-estimate variance. From a modeling point of view, these changes can be thought of as resulting from two mechanisms: one is the motion of the speckles and the other, the alterations of their morphology. In this paper, we propose a new tissue-motion estimator to counteract these speckle decorrelation effects. The estimator is based on a Lagrangian description of the speckle motion. This description allows us to follow local characteristics of the speckle field as if they were a material property. This method leads to an analytical description of the decorrelation in a way which enables the derivation of an appropriate inverse filter for speckle restoration. The filter is appropriate for linear geometrical transformation of the scattering function (LT), i.e., a constant-strain region of interest (ROI). As the LT itself is a parameter of the filter, a tissue-motion estimator can be formulated as a nonlinear minimization problem, seeking the best match between the pre-tissue-motion image and a restored-speckle post-motion image. The method is tested, using simulated radio-frequency (RF) images of tissue undergoing axial shear.

Thermal input control and enhancement for laser based residual stress measurements using liquid temperature indicating coatings

DOEpatents

Pechersky, M.J.

1999-07-06

An improved method for measuring residual stress in a material is disclosed comprising the steps of applying a spot of temperature indicating coating to the surface to be studied, establishing a speckle pattern surrounds the spot of coating with a first laser then heating the spot of coating with a far infrared laser until the surface plastically deforms. Comparing the speckle patterns before and after deformation by subtracting one pattern from the other will produce a fringe pattern that serves as a visual and quantitative indication of the degree to which the plasticized surface responded to the stress during heating and enables calculation of the stress. 3 figs.

Thermal input control and enhancement for laser based residual stress measurements using liquid temperature indicating coatings

DOEpatents

Pechersky, Martin J.

1999-01-01

An improved method for measuring residual stress in a material comprising the steps of applying a spot of temperature indicating coating to the surface to be studied, establishing a speckle pattern surrounds the spot of coating with a first laser then heating the spot of coating with a far infrared laser until the surface plastically deforms. Comparing the speckle patterns before and after deformation by subtracting one pattern from the other will produce a fringe pattern that serves as a visual and quantitative indication of the degree to which the plasticized surface responded to the stress during heating and enables calculation of the stress.

Comment on Sub-15 nm Hard X-Ray Focusing with a New Total-Reflection Zone Plate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Specht, Eliot D

2011-01-01

Takano et al. report the focusing of 10-keV X-rays to a size of 14.4 nm using a total-reflection zone plate (TRZP). This focal size is at the diffraction limit for the optic's aperture. This would be a noteworthy result, since the TRZP was fabricated using conventional lithography techniques. Alternative nanofocusing optics require more demanding fabrication methods. However, as I will discuss in this Comment, the intensity distribution presented by Takano et al. (Fig. 4 of ref. 1) is more consistent with the random speckle pattern produced by the scattering of a coherent incident beam by a distorted optic than withmore » a diffraction-limited focus. When interpreted in this manner, the true focal spot size is {approx}70 nm: 5 times the diffraction limit. When a coherent photon beam illuminates an optic containing randomly distributed regions which introduce different phase shifts, the scattered diffraction pattern consists of a speckle pattern. Each speckle will be diffraction-limited: the peak width of a single speckle depends entirely on the source coherence and gives no information about the optic. The envelope of the speckle distribution corresponds to the focal spot which would be observed using incoherent illumination. The width of this envelope is due to the finite size of the coherently-diffracting domains produced by slope and position errors in the optic. The focal intensity distribution in Fig. 4 of ref. 1 indeed contains a diffraction-limited peak, but this peak contains only a fraction of the power in the focused, and forms part of a distribution of sharp peaks with an envelope {approx}70 nm in width, just as expected for a speckle pattern. At the 4mm focal distance, the 70 nm width corresponds to a slope error of 18 {micro}rad. To reach the 14 nm diffraction limit, the slope error must be reduced to 3 {micro}rad. Takano et al. have identified a likely source of this error: warping due to stress as a result of zone deposition. It will be interesting to see whether the use of a more rigid substrate gives improved results.« less

Measuring Flow With Laser-Speckle Velocimetry

NASA Technical Reports Server (NTRS)

Smith, C. A.; Lourenco, L. M. M.; Krothapalli, A.

1988-01-01

Spatial resolution sufficient for calculation of vorticity.In laser-speckle velocimetry, pulsed or chopped laser beam expanded in one dimension by cylindrical lens to illuminate thin, fan-shaped region of flow measured. Flow seeded by small particles. Lens with optical axis perpendicular to illuminating beam forms image of illuminated particles on photographic plate. Speckle pattern of laser-illuminiated, seeded flow recorded in multiple-exposure photographs and processed to extract data on velocity field. Technique suited for study of vortical flows like those about helicopter rotor blades or airplane wings at high angles of attack.

Elastic modulus measurements of LDEF glasses and glass-ceramics using a speckle technique

NASA Technical Reports Server (NTRS)

Wiedlocher, D. E.; Kinser, D. L.

1992-01-01

Elastic moduli of five glass types and the glass-ceramic Zerodur, exposed to a near-earth orbit environment on the Long Duration Exposure Facility (LDEF), were compared to that of unexposed samples. A double exposure speckle photography technique utilizing 633 nm laser light was used in the production of the speckle pattern. Subsequent illumination of a double exposed negative using the same wavelength radiation produces Young's fringes from which the in-plane displacements are measured. Stresses imposed by compressive loading produced measurable strains in the glasses and glass-ceramic.

Speckle contrast techniques in the study of tissue thermal modification and denaturation

NASA Astrophysics Data System (ADS)

Agafonov, Dmitry N.; Kuznetsova, Liana V.; Zimnyakov, Dmitry A.; Sviridov, Alexander P.; Omelchenko, Alexander I.

2002-05-01

Results of the contrast analysis of time-averaged dynamic speckle patterns in application to monitoring of the structure modification of the thermally treated collagenous tissue such as cartilage are presented. The modification presumably induced by the bound to free water phase transition in the matrix of the treated tissue cause the specific feature of evolution of the time-averaged speckle contrast with the change of the current temperature of modified collagen tissue. This evolution appears as hysteresis associated with irreversible changes in tissue structure.

Modeling of the Light Speckle Field Structure Inside a Multilayer Human Skin Tissue

NASA Astrophysics Data System (ADS)

Barun, V. V.; Dik, S. K.; Ivanov, A. P.; Abramovich, N. D.

2013-11-01

We present an analytic method and the results of investigating the characteristics of the interference pattern formed by multiply scattered light in a multilayer biological tissue of the type of human skin at the wavelengths of the visible and neat IR spectral regions under laser irradiation. Calculations were performed with the use of the known solutions of the equations of radiation transfer in the biotissue and the relation between the theory of propagation of light in a scattering medium and the coherence theory. The radial structure of the light field in the depth of the human skin formed by coherent and incoherent radiation depending on its biophysical parameters has been investigated. The characteristic sizes of speckles in each layer of the skin have been estimated. The biophysical factors connected with the volume concentration of blood in the dermis and the degree of its oxygenation influencing the contrast of the speckle pattern in the dermis have been discussed. The possibility of formulating and solving inverse problems of biomedical optics on the restoration of blood parameters from measurements of speckle characteristics has been shown.

Determination of the stacking fault density in highly defective single GaAs nanowires by means of coherent diffraction imaging

NASA Astrophysics Data System (ADS)

Davtyan, Arman; Biermanns, Andreas; Loffeld, Otmar; Pietsch, Ullrich

2016-06-01

Coherent x-ray diffraction imaging is used to measure diffraction patterns from individual highly defective nanowires, showing a complex speckle pattern instead of well-defined Bragg peaks. The approach is tested for nanowires of 500 nm diameter and 500 nm height predominately composed by zinc-blende (ZB) and twinned zinc-blende (TZB) phase domains. Phase retrieval is used to reconstruct the measured 2-dimensional intensity patterns recorded from single nanowires with 3.48 nm and 0.98 nm spatial resolution. Whereas the speckle amplitudes and distribution are perfectly reconstructed, no unique solution could be obtained for the phase structure. The number of phase switches is found to be proportional to the number of measured speckles and follows a narrow number distribution. Using data with 0.98 nm spatial resolution the mean number of phase switches is in reasonable agreement with estimates taken from TEM. However, since the resolved phase domain still is 3-4 times larger than a single GaAs bilayer we explain the non-ambiguous phase reconstruction by the fact that depending on starting phase and sequence of subroutines used during the phase retrieval the retrieved phase domain host a different sequence of randomly stacked bilayers. Modelling possible arrangements of bilayer sequences within a phase domain demonstrate that the complex speckle patterns measured can indeed be explained by the random arrangement of the ZB and TZB phase domains.

Analysis of speckle patterns in phase-contrast images of lung tissue

NASA Astrophysics Data System (ADS)

Kitchen, M. J.; Paganin, D.; Lewis, R. A.; Yagi, N.; Uesugi, K.

2005-08-01

Propagation-based phase-contrast images of mice lungs have been obtained at the SPring-8 synchrotron research facility. Such images exhibit a speckled intensity pattern that bears a superficial resemblance to alveolar structures. This speckle results from focussing effects as projected air-filled alveoli form aberrated compound refractive lenses. An appropriate phase-retrieval algorithm has been utilized to reconstruct the approximate projected lung tissue thickness from single-phase-contrast mice chest radiographs. The results show projected density variations across the lung, highlighting regions of low density corresponding to air-filled regions. Potentially, this offers a better method than conventional radiography for detecting lung diseases such as fibrosis, emphysema and cancer, though this has yet to be demonstrated. As such, the approach can assist in continuing studies of lung function utilizing propagation-based phase-contrast imaging.

Bas-relief map using texture analysis with application to live enhancement of ultrasound images.

PubMed

Du, Huarui; Ma, Rui; Wang, Xiaoying; Zhang, Jue; Fang, Jing

2015-05-01

For ultrasound imaging, speckle is one of the most important factors in the degradation of contrast resolution because it masks meaningful texture and has the potential to interfere with diagnosis. It is expected that researchers would explore appropriate ways to reduce the speckle noise, to find the edges of structures and enhance weak borders between different organs in ultrasound imaging. Inspired by the principle of differential interference contrast microscopy, a "bas-relief map" is proposed that depicts the texture structure of ultrasound images. Based on a bas-relief map, an adaptive bas-relief filter was developed for ultrafast despeckling. Subsequently, an edge map was introduced to enhance the edges of images in real time. The holistic bas-relief map approach has been used experimentally with synthetic phantoms and digital ultrasound B-scan images of liver, kidney and gallbladder. Based on the visual inspection and the performance metrics of the despeckled images, it was found that the bas-relief map approach is capable of effectively reducing the speckle while significantly enhancing contrast and tissue boundaries for ultrasonic images, and its speckle reduction ability is comparable to that of Kuan, Lee and Frost filters. Meanwhile, the proposed technique could preserve more intra-region details compared with the popular speckle reducing anisotropic diffusion technique and more effectively enhance edges. In addition, the adaptive bas-relief filter was much less time consuming than the Kuan, Lee and Frost filter and speckle reducing anisotropic diffusion techniques. The bas-relief map strategy is effective for speckle reduction and live enhancement of ultrasound images, and can provide a valuable tool for clinical diagnosis. Copyright © 2015 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Novel phase-locked electronic speckle pattern interferometry

NASA Astrophysics Data System (ADS)

Yue, Kaiduan; Zhang, Feng; Wang, Chuangshe; Tan, Yushan

1997-03-01

The theory, design, and characteristics of a Phase-locked Electronic Speckle Pattern Interferometry (ESPI) are described. The main principle of the Phase-lock system is to use the characteristics of spatial frequency of the object light to get the information of the phase of the objects' vibration and the disturbance of air. By using the information, we eliminate not only the influence of the objects' vibration, but also the influence of the disturbance of the air. So we can get more stable image of ESPI, and more reliable measurement result.

Imaging in laser spectroscopy by a single-pixel camera based on speckle patterns

NASA Astrophysics Data System (ADS)

Žídek, K.; Václavík, J.

2016-11-01

Compressed sensing (CS) is a branch of computational optics able to reconstruct an image (or any other information) from a reduced number of measurements - thus significantly saving measurement time. It relies on encoding the detected information by a random pattern and consequent mathematical reconstruction. CS can be the enabling step to carry out imaging in many time-consuming measurements. The critical step in CS experiments is the method to invoke encoding by a random mask. Complex devices and relay optics are commonly used for the purpose. We present a new approach of creating the random mask by using laser speckles from coherent laser light passing through a diffusor. This concept is especially powerful in laser spectroscopy, where it does not require any complicated modification of the current techniques. The main advantage consist in the unmatched simplicity of the random pattern generation and a versatility of the pattern resolution. Unlike in the case of commonly used random masks, here the pattern fineness can be adjusted by changing the laser spot size being diffused. We demonstrate the pattern tuning together with the connected changes in the pattern statistics. In particular, the issue of patterns orthogonality, which is important for the CS applications, is discussed. Finally, we demonstrate on a set of 200 acquired speckle patterns that the concept can be successfully employed for single-pixel camera imaging. We discuss requirements on detector noise for the image reconstruction.

13-fold resolution gain through turbid layer via translated unknown speckle illumination

PubMed Central

Guo, Kaikai; Zhang, Zibang; Jiang, Shaowei; Liao, Jun; Zhong, Jingang; Eldar, Yonina C.; Zheng, Guoan

2017-01-01

Fluorescence imaging through a turbid layer holds great promise for various biophotonics applications. Conventional wavefront shaping techniques aim to create and scan a focus spot through the turbid layer. Finding the correct input wavefront without direct access to the target plane remains a critical challenge. In this paper, we explore a new strategy for imaging through turbid layer with a large field of view. In our setup, a fluorescence sample is sandwiched between two turbid layers. Instead of generating one focus spot via wavefront shaping, we use an unshaped beam to illuminate the turbid layer and generate an unknown speckle pattern at the target plane over a wide field of view. By tilting the input wavefront, we raster scan the unknown speckle pattern via the memory effect and capture the corresponding low-resolution fluorescence images through the turbid layer. Different from the wavefront-shaping-based single-spot scanning, the proposed approach employs many spots (i.e., speckles) in parallel for extending the field of view. Based on all captured images, we jointly recover the fluorescence object, the unknown optical transfer function of the turbid layer, the translated step size, and the unknown speckle pattern. Without direct access to the object plane or knowledge of the turbid layer, we demonstrate a 13-fold resolution gain through the turbid layer using the reported strategy. We also demonstrate the use of this technique to improve the resolution of a low numerical aperture objective lens allowing to obtain both large field of view and high resolution at the same time. The reported method provides insight for developing new fluorescence imaging platforms and may find applications in deep-tissue imaging. PMID:29359102

Unobtrusive monitoring of heart rate using a cost-effective speckle-based SI-POF remote sensor

NASA Astrophysics Data System (ADS)

Pinzón, P. J.; Montero, D. S.; Tapetado, A.; Vázquez, C.

2017-03-01

A novel speckle-based sensing technique for cost-effective heart-rate monitoring is demonstrated. This technique detects periodical changes in the spatial distribution of energy on the speckle pattern at the output of a Step-Index Polymer Optical Fiber (SI-POF) lead by using a low-cost webcam. The scheme operates in reflective configuration thus performing a centralized interrogation unit scheme. The prototype has been integrated into a mattress and its functionality has been tested with 5 different patients lying on the mattress in different positions without direct contact with the fiber sensing lead.

Optically Phase-Locked Electronic Speckle Pattern Interferometer (OPL-ESPI)

NASA Astrophysics Data System (ADS)

Moran, Steven E.; Law, Robert L.; Craig, Peter N.; Goldberg, Warren M.

1986-10-01

This report describes the design, theory, operation, and characteristics of the OPL-ESPI, which generates real time equal Doppler speckle contours of vibrating objects from unstable sensor platforms with a Doppler resolution of 30 Hz and a maximum tracking range of + or - 5 HMz. The optical phase locked loop compensates for the deleterious effects of ambient background vibration and provides the bases for a new ESPI video signal processing technique, which produces high contrast speckle contours. The OPL-ESPI system has local oscillator phase modulation capability, offering the potential for detection of vibrations with the amplitudes less than lambda/100.

Angular-domain scattering interferometry.

PubMed

Shipp, Dustin W; Qian, Ruobing; Berger, Andrew J

2013-11-15

We present an angular-scattering optical method that is capable of measuring the mean size of scatterers in static ensembles within a field of view less than 20 μm in diameter. Using interferometry, the method overcomes the inability of intensity-based models to tolerate the large speckle grains associated with such small illumination areas. By first estimating each scatterer's location, the method can model between-scatterer interference as well as traditional single-particle Mie scattering. Direct angular-domain measurements provide finer angular resolution than digitally transformed image-plane recordings. This increases sensitivity to size-dependent scattering features, enabling more robust size estimates. The sensitivity of these angular-scattering measurements to various sizes of polystyrene beads is demonstrated. Interferometry also allows recovery of the full complex scattered field, including a size-dependent phase profile in the angular-scattering pattern.

Estimation of Articular Cartilage Surface Roughness Using Gray-Level Co-Occurrence Matrix of Laser Speckle Image.

PubMed

Youssef, Doaa; El-Ghandoor, Hatem; Kandel, Hamed; El-Azab, Jala; Hassab-Elnaby, Salah

2017-06-28

The application of He-Ne laser technologies for description of articular cartilage degeneration, one of the most common diseases worldwide, is an innovative usage of these technologies used primarily in material engineering. Plain radiography and magnetic resonance imaging are insufficient to allow the early assessment of the disease. As surface roughness of articular cartilage is an important indicator of articular cartilage degeneration progress, a safe and noncontact technique based on laser speckle image to estimate the surface roughness is provided. This speckle image from the articular cartilage surface, when illuminated by laser beam, gives very important information about the physical properties of the surface. An experimental setup using a low power He-Ne laser and a high-resolution digital camera was implemented to obtain speckle images of ten bovine articular cartilage specimens prepared for different average roughness values. Texture analysis method based on gray-level co-occurrence matrix (GLCM) analyzed on the captured speckle images is used to characterize the surface roughness of the specimens depending on the computation of Haralick's texture features. In conclusion, this promising method can accurately estimate the surface roughness of articular cartilage even for early signs of degeneration. The method is effective for estimation of average surface roughness values ranging from 0.09 µm to 2.51 µm with an accuracy of 0.03 µm.

Estimation of Articular Cartilage Surface Roughness Using Gray-Level Co-Occurrence Matrix of Laser Speckle Image

PubMed Central

El-Ghandoor, Hatem; Kandel, Hamed; El-Azab, Jala; Hassab-Elnaby, Salah

2017-01-01

The application of He-Ne laser technologies for description of articular cartilage degeneration, one of the most common diseases worldwide, is an innovative usage of these technologies used primarily in material engineering. Plain radiography and magnetic resonance imaging are insufficient to allow the early assessment of the disease. As surface roughness of articular cartilage is an important indicator of articular cartilage degeneration progress, a safe and noncontact technique based on laser speckle image to estimate the surface roughness is provided. This speckle image from the articular cartilage surface, when illuminated by laser beam, gives very important information about the physical properties of the surface. An experimental setup using a low power He-Ne laser and a high-resolution digital camera was implemented to obtain speckle images of ten bovine articular cartilage specimens prepared for different average roughness values. Texture analysis method based on gray-level co-occurrence matrix (GLCM) analyzed on the captured speckle images is used to characterize the surface roughness of the specimens depending on the computation of Haralick’s texture features. In conclusion, this promising method can accurately estimate the surface roughness of articular cartilage even for early signs of degeneration. The method is effective for estimation of average surface roughness values ranging from 0.09 µm to 2.51 µm with an accuracy of 0.03 µm. PMID:28773080

Advanced one-dimensional optical strain measurement system, phase 4

NASA Technical Reports Server (NTRS)

Lant, Christian T.

1992-01-01

An improved version of the speckle-shift strain measurement system was developed. The system uses a two-dimensional sensor array to maintain speckle correlation in the presence of large off-axis rigid body motions. A digital signal processor (DSP) is used to calculate strains at a rate near the RS-170 camera frame rate. Strain measurements were demonstrated on small diameter wires and fibers used in composite materials research. Accurate values of Young's modulus were measured on tungsten wires, and silicon carbide and sapphire fibers. This optical technique has measured surface strains at specimen temperatures above 750 C and has shown the potential for measurements at much higher temperatures.

Speckle temporal stability in XAO coronagraphic images. II. Refine model for quasi-static speckle temporal evolution for VLT/SPHERE

NASA Astrophysics Data System (ADS)

Martinez, P.; Kasper, M.; Costille, A.; Sauvage, J. F.; Dohlen, K.; Puget, P.; Beuzit, J. L.

2013-06-01

Context. Observing sequences have shown that the major noise source limitation in high-contrast imaging is the presence of quasi-static speckles. The timescale on which quasi-static speckles evolve is determined by various factors, mechanical or thermal deformations, among others. Aims: Understanding these time-variable instrumental speckles and, especially, their interaction with other aberrations, referred to as the pinning effect, is paramount for the search for faint stellar companions. The temporal evolution of quasi-static speckles is, for instance, required for quantifying the gain expected when using angular differential imaging (ADI) and to determining the interval on which speckle nulling techniques must be carried out. Methods: Following an early analysis of a time series of adaptively corrected, coronagraphic images obtained in a laboratory condition with the high-order test bench (HOT) at ESO Headquarters, we confirm our results with new measurements carried out with the SPHERE instrument during its final test phase in Europe. The analysis of the residual speckle pattern in both direct and differential coronagraphic images enables the characterization of the temporal stability of quasi-static speckles. Data were obtained in a thermally actively controlled environment reproducing realistic conditions encountered at the telescope. Results: The temporal evolution of the quasi-static wavefront error exhibits a linear power law, which can be used to model quasi-static speckle evolution in the context of forthcoming high-contrast imaging instruments, with implications for instrumentation (design, observing strategies, data reduction). Such a model can be used for instance to derive the timescale on which non-common path aberrations must be sensed and corrected. We found in our data that quasi-static wavefront error increases with ~0.7 Å per minute.

Ghost Particle Velocimetry: Accurate 3D Flow Visualization Using Standard Lab Equipment

NASA Astrophysics Data System (ADS)

Buzzaccaro, Stefano; Secchi, Eleonora; Piazza, Roberto

2013-07-01

We describe and test a new approach to particle velocimetry, based on imaging and cross correlating the scattering speckle pattern generated on a near-field plane by flowing tracers with a size far below the diffraction limit, which allows reconstructing the velocity pattern in microfluidic channels without perturbing the flow. As a matter of fact, adding tracers is not even strictly required, provided that the sample displays sufficiently refractive-index fluctuations. For instance, phase separation in liquid mixtures in the presence of shear is suitable to be directly investigated by this “ghost particle velocimetry” technique, which just requires a microscope with standard lamp illumination equipped with a low-cost digital camera. As a further bonus, the peculiar spatial coherence properties of the illuminating source, which displays a finite longitudinal coherence length, allows for a 3D reconstruction of the profile with a resolution of few tenths of microns and makes the technique suitable to investigate turbid samples with negligible multiple scattering effects.

Incorrect support and missing center tolerances of phasing algorithms

DOE PAGES

Huang, Xiaojing; Nelson, Johanna; Steinbrener, Jan; ...

2010-01-01

In x-ray diffraction microscopy, iterative algorithms retrieve reciprocal space phase information, and a real space image, from an object's coherent diffraction intensities through the use of a priori information such as a finite support constraint. In many experiments, the object's shape or support is not well known, and the diffraction pattern is incompletely measured. We describe here computer simulations to look at the effects of both of these possible errors when using several common reconstruction algorithms. Overly tight object supports prevent successful convergence; however, we show that this can often be recognized through pathological behavior of the phase retrieval transfermore » function. Dynamic range limitations often make it difficult to record the central speckles of the diffraction pattern. We show that this leads to increasing artifacts in the image when the number of missing central speckles exceeds about 10, and that the removal of unconstrained modes from the reconstructed image is helpful only when the number of missing central speckles is less than about 50. In conclusion, this simulation study helps in judging the reconstructability of experimentally recorded coherent diffraction patterns.« less

Learning of speckle statistics for in vivo and noninvasive characterization of cutaneous wound regions using laser speckle contrast imaging.

PubMed

Basak, Kausik; Dey, Goutam; Mahadevappa, Manjunatha; Mandal, Mahitosh; Sheet, Debdoot; Dutta, Pranab Kumar

2016-09-01

Laser speckle contrast imaging (LSCI) provides a noninvasive and cost effective solution for in vivo monitoring of blood flow. So far, most of the researches consider changes in speckle pattern (i.e. correlation time of speckle intensity fluctuation), account for relative change in blood flow during abnormal conditions. This paper introduces an application of LSCI for monitoring wound progression and characterization of cutaneous wound regions on mice model. Speckle images are captured on a tumor wound region at mice leg in periodic interval. Initially, raw speckle images are converted to their corresponding contrast images. Functional characterization begins with first segmenting the affected area using k-means clustering, taking wavelet energies in a local region as feature set. In the next stage, different regions in wound bed are clustered based on progressive and non-progressive nature of tissue properties. Changes in contrast due to heterogeneity in tissue structure and functionality are modeled using LSCI speckle statistics. Final characterization is achieved through supervised learning of these speckle statistics using support vector machine. On cross evaluation with mice model experiment, the proposed approach classifies the progressive and non-progressive wound regions with an average sensitivity of 96.18%, 97.62% and average specificity of 97.24%, 96.42% respectively. The clinical information yield with this approach is validated with the conventional immunohistochemistry result of wound to justify the ability of LSCI for in vivo, noninvasive and periodic assessment of wounds. Copyright © 2016 Elsevier Inc. All rights reserved.

Exploiting physical constraints for multi-spectral exo-planet detection

NASA Astrophysics Data System (ADS)

Thiébaut, Éric; Devaney, Nicholas; Langlois, Maud; Hanley, Kenneth

2016-07-01

We derive a physical model of the on-axis PSF for a high contrast imaging system such as GPI or SPHERE. This model is based on a multi-spectral Taylor series expansion of the diffraction pattern and predicts that the speckles should be a combination of spatial modes with deterministic chromatic magnification and weighting. We propose to remove most of the residuals by fitting this model on a set of images at multiple wavelengths and times. On simulated data, we demonstrate that our approach achieves very good speckle suppression without additional heuristic parameters. The residual speckles1, 2 set the most serious limitation in the detection of exo-planets in high contrast coronographic images provided by instruments such as SPHERE3 at the VLT, GPI4, 5 at Gemini, or SCExAO6 at Subaru. A number of post-processing methods have been proposed to remove as much as possible of the residual speckles while preserving the signal from the planets. These methods exploit the fact that the speckles and the planetary signal have different temporal and spectral behaviors. Some methods like LOCI7 are based on angular differential imaging8 (ADI), spectral differential imaging9, 10 (SDI), or on a combination of ADI and SDI.11 Instead of working on image differences, we propose to tackle the exo-planet detection as an inverse problem where a model of the residual speckles is fit on the set of multi-spectral images and, possibly, multiple exposures. In order to reduce the number of degrees of freedom, we impose specific constraints on the spatio-spectral distribution of stellar speckles. These constraints are deduced from a multi-spectral Taylor series expansion of the diffraction pattern for an on-axis source which implies that the speckles are a combination of spatial modes with deterministic chromatic magnification and weighting. Using simulated data, the efficiency of speckle removal by fitting the proposed multi-spectral model is compared to the result of using an approximation based on the singular value decomposition of the rescaled images. We show how the difficult problem to fitting a bilinear model on the can be solved in practise. The results are promising for further developments including application to real data and joint planet detection in multi-variate data (multi-spectral and multiple exposures images).

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

PubMed Central

Malyavantham, Kishore S.; Suresh, Lakshmanan

2018-01-01

Systemic autoimmune connective tissue disorders are characterized by circulating antinuclear antibodies (ANA). Although there are several technologies available for ANA screening, indirect immunofluorescence (IIF) using Human epithelial cells-2 (HEp-2) substrate remains the primary and recommended method because of its superior sensitivity. HEp-2 substrates can detect a multitude of patterns resulting from autoantibody binding to various protein and nucleic acid autoantigens distributed throughout the nucleus and cytoplasm of the cells. The great diversity of monospecific and mixed patterns resulting from positive reactions on HEp-2 substrate also complicate the interpretation and accuracy of reporting. One specific example which received utmost attention recently is the dense fine speckled 70 (DFS70) pattern resulting from autoantibodies that specifically bind to a protein called lens epithelium derived growth factor (LEDGF). Lack of clear association with a specific systemic autoimmune disease and high prevalence in healthy populations have made accurate interpretation of DFS70 pattern important. Accurate distinction of DFS70 pattern from disease-associated patterns using conventional HEp-2 substrate is challenging. Moreover, frequent co-occurrence of DFS70 pattern along with disease-associated patterns such as homogeneous, speckled, and mixed homogeneous-speckled patterns complicate the IIF interpretation. The goal of this paper is to demonstrate the utility of a novel engineered HEp-2 IIF substrate that retains all advantages of conventional HEp-2 substrate while simultaneously providing the ability to distinguish DFS70 pattern with high confidence in both monospecific and mixed ANA positive examples. The new substrate is further able to unmask disease-associated ANA patterns previously concealed by DFS70 pattern. PMID:29364249

The agreement between 3D, standard 2D and triplane 2D speckle tracking: effects of image quality and 3D volume rate.

PubMed

Trache, Tudor; Stöbe, Stephan; Tarr, Adrienn; Pfeiffer, Dietrich; Hagendorff, Andreas

2014-12-01

Comparison of 3D and 2D speckle tracking performed on standard 2D and triplane 2D datasets of normal and pathological left ventricular (LV) wall-motion patterns with a focus on the effect that 3D volume rate (3DVR), image quality and tracking artifacts have on the agreement between 2D and 3D speckle tracking. 37 patients with normal LV function and 18 patients with ischaemic wall-motion abnormalities underwent 2D and 3D echocardiography, followed by offline speckle tracking measurements. The values of 3D global, regional and segmental strain were compared with the standard 2D and triplane 2D strain values. Correlation analysis with the LV ejection fraction (LVEF) was also performed. The 3D and 2D global strain values correlated good in both normally and abnormally contracting hearts, though systematic differences between the two methods were observed. Of the 3D strain parameters, the area strain showed the best correlation with the LVEF. The numerical agreement of 3D and 2D analyses varied significantly with the volume rate and image quality of the 3D datasets. The highest correlation between 2D and 3D peak systolic strain values was found between 3D area and standard 2D longitudinal strain. Regional wall-motion abnormalities were similarly detected by 2D and 3D speckle tracking. 2DST of triplane datasets showed similar results to those of conventional 2D datasets. 2D and 3D speckle tracking similarly detect normal and pathological wall-motion patterns. Limited image quality has a significant impact on the agreement between 3D and 2D numerical strain values.

Investigation of the Iterative Phase Retrieval Algorithm for Interferometric Applications

NASA Astrophysics Data System (ADS)

Gombkötő, Balázs; Kornis, János

2010-04-01

Sequentially recorded intensity patterns reflected from a coherently illuminated diffuse object can be used to reconstruct the complex amplitude of the scattered beam. Several iterative phase retrieval algorithms are known in the literature to obtain the initially unknown phase from these longitudinally displaced intensity patterns. When two sequences are recorded in two different states of a centimeter sized object in optical setups that are similar to digital holographic interferometry-but omitting the reference wave-, displacement, deformation, or shape measurement is theoretically possible. To do this, the retrieved phase pattern should contain information not only about the intensities and locations of the point sources of the object surface, but their relative phase as well. Not only experiments require strict mechanical precision to record useful data, but even in simulations several parameters influence the capabilities of iterative phase retrieval, such as object to camera distance range, uniform or varying camera step sequence, speckle field characteristics, and sampling. Experiments were done to demonstrate this principle with an as large as 5×5 cm sized deformable object as well. Good initial results were obtained in an imaging setup, where the intensity pattern sequences were recorded near the image plane.

Speckle photography during dynamic impact of an energetic material using laser-induced fluorescence

DOE Office of Scientific and Technical Information (OSTI.GOV)

Asay, B.W.; Laabs, G.W.; Henson, B.F.

1997-08-01

Laser and white light speckle photography have been used to observe surface displacement in a number of materials and over a varied range of strain rates. However, each suffers from limitations. We have developed a novel application of speckle photography in very difficult environments by using laser-induced fluorescence to generate the speckle pattern. This permits confinement of the free surface without undue degradation of the correlation upon which speckle methods are based. We have applied this method to measure the surface displacement of a reactive material during dynamic deformation at moderate strain rates. Conventional methods were tried but were unsuccessful,more » necessitating a novel approach. To the best of our knowledge, neither high-speed laser nor white light speckle photography has been performed using energetic materials. These measurements are very difficult because of the low material strength (yield strength {approximately}8{endash}80 MPa), and because significant out-of-plane motion and surface disruption occur during fracture, and early during the deformation process. We report results from experiments in which these major problems have been overcome. {copyright} {ital 1997 American Institute of Physics.}« less

Enhanced diagnostic of skin conditions by polarized laser speckles: phantom studies and computer modeling

NASA Astrophysics Data System (ADS)

Tchvialeva, Lioudmila; Lee, Tim K.; Markhvida, Igor; Zeng, Haishan; Doronin, Alexander; Meglinski, Igor

2014-03-01

The incidence of the skin melanoma, the most commonly fatal form of skin cancer, is increasing faster than any other potentially preventable cancer. Clinical practice is currently hampered by the lack of the ability to rapidly screen the functional and morphological properties of tissues. In our previous study we show that the quantification of scattered laser light polarization provides a useful metrics for diagnostics of the malignant melanoma. In this study we exploit whether the image speckle could improve skin cancer diagnostic in comparison with the previously used free-space speckle. The study includes skin phantom measurements and computer modeling. To characterize the depolarization of light we measure the spatial distribution of speckle patterns and analyse their depolarization ratio taken into account radial symmetry. We examine the dependences of depolarization ratio vs. roughness for phantoms which optical properties are of the order of skin lesions. We demonstrate that the variation in bulk optical properties initiates the assessable changes in the depolarization ratio. We show that image speckle differentiates phantoms significantly better than free-space speckle. The results of experimental measurements are compared with the results of Monte Carlo simulation.

Wavelet transform analysis of dynamic speckle patterns texture

NASA Astrophysics Data System (ADS)

Limia, Margarita Fernandez; Nunez, Adriana Mavilio; Rabal, Hector; Trivi, Marcelo

2002-11-01

We propose the use of the wavelet transform to characterize the time evolution of dynamic speckle patterns. We describe it by using as an example a method used for the assessment of the drying of paint. Optimal texture features are determined and the time evolution is described in terms of the Mahalanobis distance to the final (dry) state. From the behavior of this distance function, two parameters are defined that characterize the evolution. Because detailed knowledge of the involved dynamics is not required, the methodology could be implemented for other complex or poorly understood dynamic phenomena.

Expanding applications, accuracy, and interpretation of laser speckle contrast imaging of cerebral blood flow

PubMed Central

Kazmi, S M Shams; Richards, Lisa M; Schrandt, Christian J; Davis, Mitchell A; Dunn, Andrew K

2015-01-01

Laser speckle contrast imaging (LSCI) provides a rapid characterization of cortical flow dynamics for functional monitoring of the microcirculation. The technique stems from interactions of laser light with moving particles. These interactions encode the encountered Doppler phenomena within a random interference pattern imaged in widefield, known as laser speckle. Studies of neurovascular function and coupling with LSCI have benefited from the real-time characterization of functional dynamics in the laboratory setting through quantification of perfusion dynamics. While the technique has largely been relegated to acute small animal imaging, its scalability is being assessed and characterized for both chronic and clinical neurovascular imaging. PMID:25944593

Speckle correlation method used to measure object's in-plane velocity.

PubMed

Smíd, Petr; Horváth, Pavel; Hrabovský, Miroslav

2007-06-20

We present a measurement of an object's in-plane velocity in one direction by the use of the speckle correlation method. Numerical correlations of speckle patterns recorded periodically during motion of the object under investigation give information used to evaluate the object's in-plane velocity. The proposed optical setup uses a detection plane in the image field and enables one to detect the object's velocity within the interval (10-150) microm x s(-1). Simulation analysis shows a way of controlling the measuring range. The presented theory, simulation analysis, and setup are verified through an experiment of measurement of the velocity profile of an object.

Spectrally resolved digital holography using a white light LED

NASA Astrophysics Data System (ADS)

Claus, D.; Pedrini, G.; Buchta, D.; Osten, W.

2017-06-01

This paper introduces the concept of spectrally resolved digital holography. The measurement principle and the analysis of the data will be discussed in detail. The usefulness of spectrally resolved digital holography is demonstrated for colour imaging and optical metrology with regards to the recovery of modulus information and phase information, respectively. The phase information will be used to measure the shape of an object via the application of the dual wavelength method. Based on the large degree of data available, multiple speckle de-correlated dual wavelength phase maps can be obtained, which when averaged result in a signal to noise ratio improvement.

Porosity and Variations in Microgravity Aerogel Nano-Structures. 2; New Laser Speckle Characterization Methods

NASA Technical Reports Server (NTRS)

Hunt, A. J.; Ayers, M. R.; Sibille, L.; Smith, D. D.

2001-01-01

The transition from sol to gel is a process that is critical to the properties of engineered nanomaterials, but one with few available techniques for observing the dynamic processes occurring during the evolution of the gel network. Specifically, the observation of various cluster aggregation models, such as diffusion-limited and reaction-limited cluster growth can be quite difficult. This can be rather important as the actual aggregation model can dramatically influence the mechanical properties of gels, and is significantly affected by the presence of convective flows, or their absence in microgravity. We have developed two new non-intrusive optical methods for observing the aggregation processes within gels in real time. These make use of the dynamic behavior of laser speckle patterns produced when an intense laser source is passed through a gelling sol. The first method is a simplified time-correlation measurement, where the speckle pattern is observed using a CCD camera and information on the movement of the scattering objects is readily apparent. This approach is extremely sensitive to minute variations in the flow field as the observed speckle pattern is a diffraction-based image, and is therefore sensitive to motions within the sol on the order of the wavelength of the probing light. Additionally, this method has proven useful in determining a precise time for the gel-point, an event often difficult to measure. Monitoring the evolution of contrast within the speckle field is another method that has proven useful for studying aeration. In this case, speckle contrast is dependent upon the size (correlation length) and number of scattering centers, increasing with increasing size, and decreasing with increasing numbers. The dynamic behavior of cluster growth in gels causes both of these to change simultaneously with time, the exact rate of which is determined by the specific aggregation model involved. Actual growth processes can now be observed, and the effects of varying gravity fields on the growth processes qualitatively described. Results on preliminary ground-based measurements have been obtained.

Synthesis method from low-coherence digital holograms for improvement of image quality in holographic display.

PubMed

Mori, Yutaka; Nomura, Takanori

2013-06-01

In holographic displays, it is undesirable to observe the speckle noises with the reconstructed images. A method for improvement of reconstructed image quality by synthesizing low-coherence digital holograms is proposed. It is possible to obtain speckleless reconstruction of holograms due to low-coherence digital holography. An image sensor records low-coherence digital holograms, and the holograms are synthesized by computational calculation. Two approaches, the threshold-processing and the picking-a-peak methods, are proposed in order to reduce random noise of low-coherence digital holograms. The reconstructed image quality by the proposed methods is compared with the case of high-coherence digital holography. Quantitative evaluation is given to confirm the proposed methods. In addition, the visual evaluation by 15 people is also shown.

Beam uniformity analysis of infrared laser illuminators

NASA Astrophysics Data System (ADS)

Allik, Toomas H.; Dixon, Roberta E.; Proffitt, R. Patrick; Fung, Susan; Ramboyong, Len; Soyka, Thomas J.

2015-02-01

Uniform near-infrared (NIR) and short-wave infrared (SWIR) illuminators are desired in low ambient light detection, recognition, and identification of military applications. Factors that contribute to laser illumination image degradation are high frequency, coherent laser speckle and low frequency nonuniformities created by the laser or external laser cavity optics. Laser speckle analysis and beam uniformity improvements have been independently studied by numerous authors, but analysis to separate these two effects from a single measurement technique has not been published. In this study, profiles of compact, diode laser NIR and SWIR illuminators were measured and evaluated. Digital 12-bit images were recorded with a flat-field calibrated InGaAs camera with measurements at F/1.4 and F/16. Separating beam uniformity components from laser speckle was approximated by filtering the original image. The goal of this paper is to identify and quantify the beam quality variation of illumination prototypes, draw awareness to its impact on range performance modeling, and develop measurement techniques and methodologies for military, industry, and vendors of active sources.

Experimental research of digital image correlation system in high temperature test

NASA Astrophysics Data System (ADS)

Chen, Li; Wang, Yonghong; Dan, Xizuo; Xiao, Ying; Yang, Lianxiang

2016-01-01

Digital Image Correlation (DIC) is a full-field technique based on white-light illumination for displacement and strain measurement. But radiation on the specimen surface at high temperature affects the quality of acquired speckle pattern images for traditional DIC measurement. In order to minimize the radiation effect in high temperature measurement, this paper proposes a two-dimensional ultraviolet digital image correlation system (2D UV-DIC) containing UV LED and UV band-pass filter. It is confirmed by experiments that images acquired by this system saturate at higher temperature in comparison with DIC using filtered blue light imaging system. And the UV-DIC remains minimally affected by radiation at the temperature which is nearing the specimen's maximum working temperature (about 1250°C). In addition, considering the heat disturbance that can't be ignored in actual high temperature measurement, this paper also proposes a method using an air controller in combination with image average algorithm, and the method was then used to obtain the thermal expansion coefficient of the Austenitic chromium-nickel stainless steel specimen at different temperatures. By comparing the coefficients with the results calculated by other method, it shows that this comprehensive method has the advantages of strong anti-interference ability and high precision.

Theoretical investigations on dual-beam illumination electronic speckle pattern interferometry

NASA Astrophysics Data System (ADS)

Goudemand, Nicolas

2006-07-01

Contrary to what is found in most of the existing scientific literature, where a specific frame is developed, the theory of speckle interferometry is (conveniently) presented here as a particular case of the more general theory of holographic interferometry. In addition to the intellectual benefit of dealing with a single unified theory, this brings about many advantages when it comes to discuss fundamental topics such as the three-dimensional evolution of the complex amplitude of the diffuse optical wavefronts, the degree of approximation of the leading formulas, the loss of fringe contrast, the decorrelation effects, the real influence of the terms generally neglected in out-of-focus regions. In the same way, the statistical properties of the speckle fields, usually treated as a separate subject matter, are also integrated in the theory, thus providing a comprehensive knowledge of the qualitative features of speckle interferometry methods, otherwise difficult to understand.

Dynamic speckle study of microbial growth

NASA Astrophysics Data System (ADS)

Vincitorio, F. M.; Mulone, C.; Marcuzzi, P. A.; Budini, N.; Freyre, C.; Lopez, A. J.; Ramil, A.

2015-08-01

In this work we present a characterization of yeast dynamic speckle activity during growth in an isolated agar culture medium. We found that it is possible to detect the growth of the microorganisms even before they turn out to be visible. By observing the time evolution of the speckle activity at different regions of the culture medium we could extract a map of the growth process, which served to analyze how the yeast develops and spreads over the agar's medium. An interesting point of this study concerns with the influence of the laser light on the yeast growth rate. We have found that yeast finds hard to develop at regions with higher laser light illumination, although we used a synchronous system to capture the speckle pattern. The results obtained in this work would serve us as a starting point to fabricate a detector of growing microorganism colonies, with obvious interesting applications in diverse areas.

Variance based joint sparsity reconstruction of synthetic aperture radar data for speckle reduction

NASA Astrophysics Data System (ADS)

Scarnati, Theresa; Gelb, Anne

2018-04-01

In observing multiple synthetic aperture radar (SAR) images of the same scene, it is apparent that the brightness distributions of the images are not smooth, but rather composed of complicated granular patterns of bright and dark spots. Further, these brightness distributions vary from image to image. This salt and pepper like feature of SAR images, called speckle, reduces the contrast in the images and negatively affects texture based image analysis. This investigation uses the variance based joint sparsity reconstruction method for forming SAR images from the multiple SAR images. In addition to reducing speckle, the method has the advantage of being non-parametric, and can therefore be used in a variety of autonomous applications. Numerical examples include reconstructions of both simulated phase history data that result in speckled images as well as the images from the MSTAR T-72 database.

Demonstration of a Speckle Based Sensing with Pulse-Doppler Radar for Vibration Detection.

PubMed

Ozana, Nisan; Bauer, Reuven; Ashkenazy, Koby; Sasson, Nissim; Schwarz, Ariel; Shemer, Amir; Zalevsky, Zeev

2018-05-03

In previous works, an optical technique for extraction and separation of remote static vibrations has been demonstrated. In this paper, we will describe an approach in which RF speckle movement is used to extract remote vibrations of a static target. The use of conventional radar Doppler methods is not suitable for detecting vibrations of static targets. In addition, the speckle method has an important advantage, in that it is able to detect vibrations at far greater distances than what is normally detected in classical optical methods. The experiment described in this paper was done using a motorized vehicle, which engine was turned on and off. The results showed that the system was able to distinguish between the different engine states, and in addition, was able to determine the vibration frequency of the engine. The first step towards real time detection of human vital signs using RF speckle patterns is presented.

Speckle reduction in optical coherence tomography images based on wave atoms

PubMed Central

Du, Yongzhao; Liu, Gangjun; Feng, Guoying; Chen, Zhongping

2014-01-01

Abstract. Optical coherence tomography (OCT) is an emerging noninvasive imaging technique, which is based on low-coherence interferometry. OCT images suffer from speckle noise, which reduces image contrast. A shrinkage filter based on wave atoms transform is proposed for speckle reduction in OCT images. Wave atoms transform is a new multiscale geometric analysis tool that offers sparser expansion and better representation for images containing oscillatory patterns and textures than other traditional transforms, such as wavelet and curvelet transforms. Cycle spinning-based technology is introduced to avoid visual artifacts, such as Gibbs-like phenomenon, and to develop a translation invariant wave atoms denoising scheme. The speckle suppression degree in the denoised images is controlled by an adjustable parameter that determines the threshold in the wave atoms domain. The experimental results show that the proposed method can effectively remove the speckle noise and improve the OCT image quality. The signal-to-noise ratio, contrast-to-noise ratio, average equivalent number of looks, and cross-correlation (XCOR) values are obtained, and the results are also compared with the wavelet and curvelet thresholding techniques. PMID:24825507

Real-time blood flow visualization using the graphics processing unit

NASA Astrophysics Data System (ADS)

Yang, Owen; Cuccia, David; Choi, Bernard

2011-01-01

Laser speckle imaging (LSI) is a technique in which coherent light incident on a surface produces a reflected speckle pattern that is related to the underlying movement of optical scatterers, such as red blood cells, indicating blood flow. Image-processing algorithms can be applied to produce speckle flow index (SFI) maps of relative blood flow. We present a novel algorithm that employs the NVIDIA Compute Unified Device Architecture (CUDA) platform to perform laser speckle image processing on the graphics processing unit. Software written in C was integrated with CUDA and integrated into a LabVIEW Virtual Instrument (VI) that is interfaced with a monochrome CCD camera able to acquire high-resolution raw speckle images at nearly 10 fps. With the CUDA code integrated into the LabVIEW VI, the processing and display of SFI images were performed also at ~10 fps. We present three video examples depicting real-time flow imaging during a reactive hyperemia maneuver, with fluid flow through an in vitro phantom, and a demonstration of real-time LSI during laser surgery of a port wine stain birthmark.

Real-time blood flow visualization using the graphics processing unit

PubMed Central

Yang, Owen; Cuccia, David; Choi, Bernard

2011-01-01

Laser speckle imaging (LSI) is a technique in which coherent light incident on a surface produces a reflected speckle pattern that is related to the underlying movement of optical scatterers, such as red blood cells, indicating blood flow. Image-processing algorithms can be applied to produce speckle flow index (SFI) maps of relative blood flow. We present a novel algorithm that employs the NVIDIA Compute Unified Device Architecture (CUDA) platform to perform laser speckle image processing on the graphics processing unit. Software written in C was integrated with CUDA and integrated into a LabVIEW Virtual Instrument (VI) that is interfaced with a monochrome CCD camera able to acquire high-resolution raw speckle images at nearly 10 fps. With the CUDA code integrated into the LabVIEW VI, the processing and display of SFI images were performed also at ∼10 fps. We present three video examples depicting real-time flow imaging during a reactive hyperemia maneuver, with fluid flow through an in vitro phantom, and a demonstration of real-time LSI during laser surgery of a port wine stain birthmark. PMID:21280915

Full-wave and ray-based modeling of cross-beam energy transfer between laser beams with distributed phase plates and polarization smoothing

DOE PAGES

Follett, R. K.; Edgell, D. H.; Froula, D. H.; ...

2017-10-20

Radiation-hydrodynamic simulations of inertial confinement fusion (ICF) experiments rely on ray-based cross-beam energy transfer (CBET) models to calculate laser energy deposition. The ray-based models assume locally plane-wave laser beams and polarization averaged incoherence between laser speckles for beams with polarization smoothing. The impact of beam speckle and polarization smoothing on crossbeam energy transfer (CBET) are studied using the 3-D wave-based laser-plasma-interaction code LPSE. The results indicate that ray-based models under predict CBET when the assumption of spatially averaged longitudinal incoherence across the CBET interaction region is violated. A model for CBET between linearly-polarized speckled beams is presented that uses raymore » tracing to solve for the real speckle pattern of the unperturbed laser beams within the eikonal approximation and gives excellent agreement with the wavebased calculations. Lastly, OMEGA-scale 2-D LPSE calculations using ICF relevant plasma conditions suggest that the impact of beam speckle on laser absorption calculations in ICF implosions is small (< 1%).« less

Speckle reduction of OCT images using an adaptive cluster-based filtering

NASA Astrophysics Data System (ADS)

Adabi, Saba; Rashedi, Elaheh; Conforto, Silvia; Mehregan, Darius; Xu, Qiuyun; Nasiriavanaki, Mohammadreza

2017-02-01

Optical coherence tomography (OCT) has become a favorable device in the dermatology discipline due to its moderate resolution and penetration depth. OCT images however contain grainy pattern, called speckle, due to the broadband source that has been used in the configuration of OCT. So far, a variety of filtering techniques is introduced to reduce speckle in OCT images. Most of these methods are generic and can be applied to OCT images of different tissues. In this paper, we present a method for speckle reduction of OCT skin images. Considering the architectural structure of skin layers, it seems that a skin image can benefit from being segmented in to differentiable clusters, and being filtered separately in each cluster by using a clustering method and filtering methods such as Wiener. The proposed algorithm was tested on an optical solid phantom with predetermined optical properties. The algorithm was also tested on healthy skin images. The results show that the cluster-based filtering method can reduce the speckle and increase the signal-to-noise ratio and contrast while preserving the edges in the image.

Ultrasound speckle reduction based on fractional order differentiation.

PubMed

Shao, Dangguo; Zhou, Ting; Liu, Fan; Yi, Sanli; Xiang, Yan; Ma, Lei; Xiong, Xin; He, Jianfeng

2017-07-01

Ultrasound images show a granular pattern of noise known as speckle that diminishes their quality and results in difficulties in diagnosis. To preserve edges and features, this paper proposes a fractional differentiation-based image operator to reduce speckle in ultrasound. An image de-noising model based on fractional partial differential equations with balance relation between k (gradient modulus threshold that controls the conduction) and v (the order of fractional differentiation) was constructed by the effective combination of fractional calculus theory and a partial differential equation, and the numerical algorithm of it was achieved using a fractional differential mask operator. The proposed algorithm has better speckle reduction and structure preservation than the three existing methods [P-M model, the speckle reducing anisotropic diffusion (SRAD) technique, and the detail preserving anisotropic diffusion (DPAD) technique]. And it is significantly faster than bilateral filtering (BF) in producing virtually the same experimental results. Ultrasound phantom testing and in vivo imaging show that the proposed method can improve the quality of an ultrasound image in terms of tissue SNR, CNR, and FOM values.

Manipulation of long-term dynamics in a colloidal active matter system using speckle light fields

NASA Astrophysics Data System (ADS)

Pince, Ercag; Velu, Sabareesh K. P.; Callegari, Agnese; Elahi, Parviz; Gigan, Sylvain; Volpe, Giovanni; Volpe, Giorgio

Particles undergoing a stochastic motion within a disordered medium is a ubiquitous physical and biological phenomena. Examples can be given from organelles performing tasks in the cytoplasm to large animals moving in patchy environment. Here, we use speckle light fields to study the anomalous diffusion in an active matter system consisting of micron-sized silica particles(diameter 5 μm) and motile bacterial cells (E. coli). The speckle light fields are generated by mode mixing inside a multimode optical fiber where a small amount of incident laser power is needed to obtain an effective disordered optical landscape for the purpose of optical manipulation. We experimentally show how complex potentials contribute to the long-term dynamics of the active matter system and observed an enhanced diffusion of particles interacting with the active bacterial bath in the speckle light fields. We showed that this effect can be tuned and controlled by varying the intensity and the statistical properties of the speckle pattern. Potentially, these results could be of interest for many technological applications, such as the manipulation of microparticles inside optically disordered media of biological interest.

Full-wave and ray-based modeling of cross-beam energy transfer between laser beams with distributed phase plates and polarization smoothing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Follett, R. K.; Edgell, D. H.; Froula, D. H.

Radiation-hydrodynamic simulations of inertial confinement fusion (ICF) experiments rely on ray-based cross-beam energy transfer (CBET) models to calculate laser energy deposition. The ray-based models assume locally plane-wave laser beams and polarization averaged incoherence between laser speckles for beams with polarization smoothing. The impact of beam speckle and polarization smoothing on crossbeam energy transfer (CBET) are studied using the 3-D wave-based laser-plasma-interaction code LPSE. The results indicate that ray-based models under predict CBET when the assumption of spatially averaged longitudinal incoherence across the CBET interaction region is violated. A model for CBET between linearly-polarized speckled beams is presented that uses raymore » tracing to solve for the real speckle pattern of the unperturbed laser beams within the eikonal approximation and gives excellent agreement with the wavebased calculations. Lastly, OMEGA-scale 2-D LPSE calculations using ICF relevant plasma conditions suggest that the impact of beam speckle on laser absorption calculations in ICF implosions is small (< 1%).« less

New techniques for fluorescence background rejection in microscopy and endoscopy

NASA Astrophysics Data System (ADS)

Ventalon, Cathie

2009-03-01

Confocal microscopy is a popular technique in the bioimaging community, mainly because it provides optical sectioning. However, its standard implementation requires 3-dimensional scanning of focused illumination throughout the sample. Efficient non-scanning alternatives have been implemented, among which the simple and well-established incoherent structured illumination microscopy (SIM) [1]. We recently proposed a similar technique, called Dynamic Speckle Illumination (DSI) microscopy, wherein the incoherent grid illumination pattern is replaced with a coherent speckle illumination pattern from a laser, taking advantage of the fact that speckle contrast is highly maintained in a scattering media, making the technique well adapted to tissue imaging [2]. DSI microscopy relies on the illumination of a sample with a sequence of dynamic speckle patterns and an image processing algorithm based only on an a priori knowledge of speckle statistics. The choice of this post-processing algorithm is crucial to obtain a good sectioning strength: in particular, we developed a novel post-processing algorithm based one wavelet pre-filtering of the raw images and obtained near-confocal fluorescence sectioning in a mouse brain labeled with GFP, with a good image quality maintained throughout a depth of ˜100 μm [3]. In the purpose of imaging fluorescent tissue at higher depth, we recently applied structured illumination to endoscopy. We used a similar set-up wherein the illumination pattern (a one-dimensional grid) is transported to the sample with an imaging fiber bundle with miniaturized objective and the fluorescence image is collected through the same bundle. Using a post-processing algorithm similar to the one previously described [3], we obtained high-quality images of a fluorescein-labeled rat colonic mucosa [4], establishing the potential of our endomicroscope for bioimaging applications. [4pt] Ref: [0pt] [1] M. A. A. Neil et al, Opt. Lett. 22, 1905 (1997) [0pt] [2] C. Ventalon et al, Opt. Lett. 30, 3350 (2005) [0pt] [3] C. Ventalon et al, Opt. Lett. 32, 1417 (2007) [0pt] [4] N. Bozinovic et al, Opt. Express 16, 8016 (2008)

Application of biospeckle phenomenon on monitoring of leavening process in breadmaking

NASA Astrophysics Data System (ADS)

da Silva, Emerson Rodrigo; da Silva Junior, Elieste; Júnior, Mauro Favoretto; da Silva Lannes, Suzana Caetano; Muramatsu, Mikiya

2008-04-01

Since the sixties, dynamic speckles have found a wide number of applications, covering fields from engineering to biomedicine. However, despite of this potential, its utilisation in food manufacture control is still incipient. In this work, our objective was to use the biospeckle phenomenon to monitoring the expansion of the dough during the leavening process. Our experiments consisted in the temporal evolution observation of speckle patterns scattered by samples prepared from two formulations: by yoghurt addition and without it. Every two minutes, it was constructed a 2-D image, recording the Time History Speckle Pattern (THSP). The full monitoring time was 50 minutes. A new estimator of activity index, proposed by some of the authors, was used in data analysis. This estimator is based on contrast of successive correlations of intensities recorded in THSP. It was found a strong correlation between the activities on the samples and the type of fermentative agents. These results reveal an interesting potential of the biospeckle to control in bread manufacture industries.

Constellation of phase singularities in a speckle-like pattern for optical vortex metrology applied to biological kinematic analysis.

PubMed

Wang, Wei; Qiao, Yu; Ishijima, Reika; Yokozeki, Tomoaki; Honda, Daigo; Matsuda, Akihiro; Hanson, Steen G; Takeda, Mitsuo

2008-09-01

A novel technique for biological kinematic analysis is proposed that makes use of the pseudophase singularities in a complex signal generated from a speckle-like pattern. In addition to the information about the locations and the anisotropic core structures of the pseudophase singularities, we also detect the spatial structures of a cluster of phase singularities, which serves as a unique constellation characterizing the mutual position relation between the individual pseudophase singularities. Experimental results of in vivo measurements for a swimming fish along with its kinematic analysis are presented, which demonstrate the validity of the proposed technique.

Laser-speckle-visibility acoustic spectroscopy in soft turbid media.

PubMed

Wintzenrieth, Frédéric; Cohen-Addad, Sylvie; Le Merrer, Marie; Höhler, Reinhard

2014-01-01

We image the evolution in space and time of an acoustic wave propagating along the surface of turbid soft matter by shining coherent light on the sample. The wave locally modulates the speckle interference pattern of the backscattered light, which is recorded using a camera. We show both experimentally and theoretically how the temporal and spatial correlations in this pattern can be analyzed to obtain the acoustic wavelength and attenuation length. The technique is validated using shear waves propagating in aqueous foam. It may be applied to other kinds of acoustic waves in different forms of turbid soft matter such as biological tissues, pastes, or concentrated emulsions.

Laser-speckle-visibility acoustic spectroscopy in soft turbid media

NASA Astrophysics Data System (ADS)

Wintzenrieth, Frédéric; Cohen-Addad, Sylvie; Le Merrer, Marie; Höhler, Reinhard

2014-01-01

We image the evolution in space and time of an acoustic wave propagating along the surface of turbid soft matter by shining coherent light on the sample. The wave locally modulates the speckle interference pattern of the backscattered light, which is recorded using a camera. We show both experimentally and theoretically how the temporal and spatial correlations in this pattern can be analyzed to obtain the acoustic wavelength and attenuation length. The technique is validated using shear waves propagating in aqueous foam. It may be applied to other kinds of acoustic waves in different forms of turbid soft matter such as biological tissues, pastes, or concentrated emulsions.

The use of Electronic Speckle Pattern Interferometry (ESPI) in the crack propagation analysis of epoxy resins

NASA Astrophysics Data System (ADS)

Herbert, D. P.; Al-Hassani, A. H. M.; Richardson, M. O. W.

The ESPI (electronic speckle pattern interferometry) technique at high magnification levels is demonstrated to be of considerable value in interpreting the fracture behaviour of epoxy resins. The fracture toughness of powder coating system at different thicknesses has been measured using a TDCB (tapered double cantilever beam) technique and the deformation zone at the tip of the moving crack monitored. Initial indications are that a mechanistic changeover occurs at a critical bond (coating) thickness and that this is synonymous with the occurence of a fracture toughness maximum, which in turn is associated with a deformation zone of specific diameter.

Detection of fungi colony growth on bones by dynamic speckle

NASA Astrophysics Data System (ADS)

Vincitorio, F. M.; Budini, N.; Mulone, C.; Spector, M.; Freyre, C.; López Díaz, A. J.; Ramil, A.

2013-11-01

In this work we have studied the dynamic speckle patterns of mucor fungi colonies, which were inoculated on different samples. We were interested in analyzing the development of fungi colonies in bones, since during the last two years, a series of infections by mucor fungi have been reported on patients from different hospitals in Argentina. Coincidentally, all of these infections appeared on patients that were subjected to a surgical intervention for implantation of a titanium prosthesis. Apparently, the reason of the infection was a deficient sterilization process in conjunction with an accidental contamination. We observed that fungi growth, activity and death can be distinguished by means of the dynamic speckle technique.

Measurement of dynamic patterns of an elastic membrane at bi-modal vibration using high speed electronic speckle pattern interferometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Preciado, Jorge Sanchez; Lopez, Carlos Perez; Santoyo, Fernando Mendoza

2014-05-27

Implementing a hybrid arrangement of Laser Doppler Vibrometry (LDV) and high speed Electronic Speckle Pattern Interferometry (ESPI) we were able to measure the dynamic patterns of a flat rectangular elastic membrane clamped at its edges stimulated with the sum of two resonance frequencies. ESPI is a versatile technique to analyze in real-time the deformation of a membrane since its low computational cost and easy implementation of the optical setup. Elastic membranes present nonlinear behaviors when stimulated with low amplitude signals. The elastic membrane under test, with several non rational related vibrating modals below the 200 Hz, was stimulated with twomore » consecutives resonant frequencies. The ESPI patterns, acquired at high speed rates, shown a similar behavior for the dual frequency stimulation as in the case of patterns formed with the entrainment frequency. We think this may be related to the effects observed in the application of dual frequency stimulation in ultrasound.« less

Flaw Detection and Evaluation of Composite Cylinders Using Laser Speckle Interferometry and Holography

DTIC Science & Technology

1979-11-23

Entered) ACKNOWLEDGMENTS The author hereby expresses his appreciation to Mr. J. A. Schaeffel Jr. for his guidance on interferometry and the computer...were collected by an automated laser speckle interferometry displacement contour analyzer developed by John A. Schaeffel , Jr. [3]. The new method of 10...Fringe Patterns, US Army Missile Command, Redstone Arsenal, Alabama, Technical Report RL-76-18, 20 April 1976. 3. Schaeffel , J. A., Automated Laser

Digital Image Speckle Correlation for the Quantification of the Cosmetic Treatment with Botulinum Toxin Type A (BTX-A)

NASA Astrophysics Data System (ADS)

Bhatnagar, Divya; Conkling, Nicole; Rafailovich, Miriam; Dagum, Alexander

2012-02-01

The skin on the face is directly attached to the underlying muscles. Here, we successfully introduce a non-invasive, non-contact technique, Digital Image Speckle Correlation (DISC), to measure the precise magnitude and duration of facial muscle paralysis inflicted by BTX-A. Subjective evaluation by clinicians and patients fail to objectively quantify the direct effect and duration of BTX-A on the facial musculature. By using DISC, we can (a) Directly measure deformation field of the facial skin and determine the locus of facial muscular tension(b)Quantify and monitor muscular paralysis and subsequent re-innervation following injection; (c) Continuously correlate the appearance of wrinkles and muscular tension. Two sequential photographs of slight facial motion (frowning, raising eyebrows) are taken. DISC processes the images to produce a vector map of muscular displacement from which spatially resolved information is obtained regarding facial tension. DISC can track the ability of different muscle groups to contract and can be used to predict the site of injection, quantify muscle paralysis and the rate of recovery following BOTOX injection.

Vibrational Analysis of Engine Components Using Neural-Net Processing and Electronic Holography

NASA Technical Reports Server (NTRS)

Decker, Arthur J.; Fite, E. Brian; Mehmed, Oral; Thorp, Scott A.

1997-01-01

The use of computational-model trained artificial neural networks to acquire damage specific information from electronic holograms is discussed. A neural network is trained to transform two time-average holograms into a pattern related to the bending-induced-strain distribution of the vibrating component. The bending distribution is very sensitive to component damage unlike the characteristic fringe pattern or the displacement amplitude distribution. The neural network processor is fast for real-time visualization of damage. The two-hologram limit makes the processor more robust to speckle pattern decorrelation. Undamaged and cracked cantilever plates serve as effective objects for testing the combination of electronic holography and neural-net processing. The requirements are discussed for using finite-element-model trained neural networks for field inspections of engine components. The paper specifically discusses neural-network fringe pattern analysis in the presence of the laser speckle effect and the performances of two limiting cases of the neural-net architecture.

Vibrational Analysis of Engine Components Using Neural-Net Processing and Electronic Holography

NASA Technical Reports Server (NTRS)

Decker, Arthur J.; Fite, E. Brian; Mehmed, Oral; Thorp, Scott A.

1998-01-01

The use of computational-model trained artificial neural networks to acquire damage specific information from electronic holograms is discussed. A neural network is trained to transform two time-average holograms into a pattern related to the bending-induced-strain distribution of the vibrating component. The bending distribution is very sensitive to component damage unlike the characteristic fringe pattern or the displacement amplitude distribution. The neural network processor is fast for real-time visualization of damage. The two-hologram limit makes the processor more robust to speckle pattern decorrelation. Undamaged and cracked cantilever plates serve as effective objects for testing the combination of electronic holography and neural-net processing. The requirements are discussed for using finite-element-model trained neural networks for field inspections of engine components. The paper specifically discusses neural-network fringe pattern analysis in the presence of the laser speckle effect and the performances of two limiting cases of the neural-net architecture.

Fast computation algorithms for speckle pattern simulation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nascov, Victor; Samoilă, Cornel; Ursuţiu, Doru

2013-11-13

We present our development of a series of efficient computation algorithms, generally usable to calculate light diffraction and particularly for speckle pattern simulation. We use mainly the scalar diffraction theory in the form of Rayleigh-Sommerfeld diffraction formula and its Fresnel approximation. Our algorithms are based on a special form of the convolution theorem and the Fast Fourier Transform. They are able to evaluate the diffraction formula much faster than by direct computation and we have circumvented the restrictions regarding the relative sizes of the input and output domains, met on commonly used procedures. Moreover, the input and output planes canmore » be tilted each to other and the output domain can be off-axis shifted.« less

Laser Speckle Contrast Imaging: theory, instrumentation and applications.

PubMed

Senarathna, Janaka; Rege, Abhishek; Li, Nan; Thakor, Nitish V

2013-01-01

Laser Speckle Contrast Imaging (LSCI) is a wide field of view, non scanning optical technique for observing blood flow. Speckles are produced when coherent light scattered back from biological tissue is diffracted through the limiting aperture of focusing optics. Mobile scatterers cause the speckle pattern to blur; a model can be constructed by inversely relating the degree of blur, termed speckle contrast to the scatterer speed. In tissue, red blood cells are the main source of moving scatterers. Therefore, blood flow acts as a virtual contrast agent, outlining blood vessels. The spatial resolution (~10 μm) and temporal resolution (10 ms to 10 s) of LSCI can be tailored to the application. Restricted by the penetration depth of light, LSCI can only visualize superficial blood flow. Additionally, due to its non scanning nature, LSCI is unable to provide depth resolved images. The simple setup and non-dependence on exogenous contrast agents have made LSCI a popular tool for studying vascular structure and blood flow dynamics. We discuss the theory and practice of LSCI and critically analyze its merit in major areas of application such as retinal imaging, imaging of skin perfusion as well as imaging of neurophysiology.

Quantification and Reconstruction in Photoacoustic Tomography

NASA Astrophysics Data System (ADS)

Guo, Zijian

Optical absorption is closely associated with many physiological important parameters, such as the concentration and oxygen saturation of hemoglobin. Conventionally, accurate quantification in PAT requires knowledge of the optical fluence attenuation, acoustic pressure attenuation, and detection bandwidth. We circumvent this requirement by quantifying the optical absorption coefficients from the acoustic spectra of PA signals acquired at multiple optical wavelengths. We demonstrate the method using the optical-resolution photoacoustic microscopy (OR-PAM) and the acoustical-resolution photoacoustic microscopy (AR-PAM) in the optical ballistic regime and in the optical diffusive regime, respectively. The data acquisition speed in photoacoustic computed tomography (PACT) is limited by the laser repetition rate and the number of parallel ultrasound detecting channels. Reconstructing an image with fewer measurements can effectively accelerate the data acquisition and reduce the system cost. We adapted Compressed Sensing (CS) for the reconstruction in PACT. CS-based PACT was implemented as a non-linear conjugate gradient descent algorithm and tested with both phantom and in vivo experiments. Speckles have been considered ubiquitous in all scattering-based coherent imaging technologies. As a coherent imaging modality based on optical absorption, photoacoustic (PA) tomography (PAT) is generally devoid of speckles. PAT suppresses speckles by building up prominent boundary signals, via a mechanism similar to that of specular reflection. When imaging smooth boundary absorbing targets, the speckle visibility in PAT, which is defined as the ratio of the square root of the average power of speckles to that of boundaries, is inversely proportional to the square root of the absorber density. If the surfaces of the absorbing targets have uncorrelated height fluctuations, however, the boundary features may become fully developed speckles. The findings were validated by simulations and experiments. The first- and second-order statistics of PAT speckles were also studied experimentally. While the amplitude of the speckles follows a Gaussian distribution, the autocorrelation of the speckle patterns tracks that of the system point spread function.

Application of speckle image correlation for real-time assessment of metabolic activity in herpes virus-infected cells

NASA Astrophysics Data System (ADS)

Vladimirov, A. P.; Malygin, A. S.; Mikhailova, J. A.; Borodin, E. M.; Bakharev, A. A.; Poryvayeva, A. P.

2014-09-01

Earlier we reported developing a speckle interferometry technique and a device designed to assess the metabolic activity of a cell monolayer cultivated on a glass substrate. This paper aimed at upgrading the technique and studying its potential for real-time assessment of herpes virus development process. Speckle dynamics was recorded in the image plane of intact and virus-infected cell monolayer. HLE-3, L-41 and Vero cells were chosen as research targets. Herpes simplex virus-1-(HSV-1)- infected cell cultures were studied. For 24 h we recorded the digital value of optical signal I in one pixel and parameter η characterizing change in the distribution of the optical signal on 10 × 10-pixel areas. The coefficient of multiple determination calculated by η time dependences for three intact cell cultures equals 0.94. It was demonstrated that the activity parameters are significantly different for intact and virus-infected cells. The difference of η value for intact and HSV-1-infected cells is detectable 10 minutes from the experiment start.

MATLAB for laser speckle contrast analysis (LASCA): a practice-based approach

NASA Astrophysics Data System (ADS)

Postnikov, Eugene B.; Tsoy, Maria O.; Postnov, Dmitry E.

2018-04-01

Laser Speckle Contrast Analysis (LASCA) is one of the most powerful modern methods for revealing blood dynamics. The experimental design and theory for this method are well established, and the computational recipie is often regarded to be trivial. However, the achieved performance and spatial resolution may considerable differ for different implementations. We comprise a minireview of known approaches to the spatial laser speckle contrast data processing and their realization in MATLAB code providing an explicit correspondence to the mathematical representation, a discussion of available implementations. We also present the algorithm based on the 2D Haar wavelet transform, also supplied with the program code. This new method provides an opportunity to introduce horizontal, vertical and diagonal speckle contrasts; it may be used for processing highly anisotropic images of vascular trees. We provide the comparative analysis of the accuracy of vascular pattern detection and the processing times with a special attention to details of the used MATLAB procedures.

Cost-efficient speckle interferometry with plastic optical fiber for unobtrusive monitoring of human vital signs.

PubMed

Podbreznik, Peter; Đonlagić, Denis; Lešnik, Dejan; Cigale, Boris; Zazula, Damjan

2013-10-01

A cost-efficient plastic optical fiber (POF) system for unobtrusive monitoring of human vital signs is presented. The system is based on speckle interferometry. A laser diode is butt-coupled to the POF whose exit face projects speckle patterns onto a linear optical sensor array. Sequences of acquired speckle images are transformed into one-dimensional signals by using the phase-shifting method. The signals are analyzed by band-pass filtering and a Morlet-wavelet-based multiresolutional approach for the detection of cardiac and respiratory activities, respectively. The system is tested with 10 healthy nonhospitalized persons, lying supine on a mattress with the embedded POF. Experimental results are assessed statistically: precisions of 98.8% ± 1.5% and 97.9% ± 2.3%, sensitivities of 99.4% ± 0.6% and 95.3% ± 3%, and mean delays between interferometric detections and corresponding referential signals of 116.6 ± 55.5 and 1299.2 ± 437.3 ms for the heartbeat and respiration are obtained, respectively.

Self-mixing instrument for simultaneous distance and speed measurement

NASA Astrophysics Data System (ADS)

Norgia, Michele; Melchionni, Dario; Pesatori, Alessandro

2017-12-01

A novel instrument based on Self-mixing interferometry is proposed to simultaneously measure absolute distance and velocity. The measurement method is designed for working directly on each kind of surface, in industrial environment, overcoming also problems due to speckle pattern effect. The laser pump current is modulated at quite high frequency (40 kHz) and the estimation of the induced fringes frequency allows an almost instantaneous measurement (measurement time equal to 25 μs). A real time digital elaboration processes the measurement data and discards unreliable measurements. The simultaneous measurement reaches a relative standard deviation of about 4·10-4 in absolute distance, and 5·10-3 in velocity measurement. Three different laser sources are tested and compared. The instrument shows good performances also in harsh environment, for example measuring the movement of an opaque iron tube rotating under a running water flow.

Assessment of Fatigue Resistance of Aluminide Layers on MAR 247 Nickel Super Alloy with Full-Field Optical Strain Measurements

NASA Astrophysics Data System (ADS)

Kukla, D.; Brynk, T.; Pakieła, Z.

2017-08-01

This work presents the results of fatigue tests of MAR 247 alloy flat specimens with aluminides layers of 20 or 40 µm thickness obtained in CVD process. Fatigue test was conducted at amplitude equal to half of maximum load and ranging between 300 and 650 MPa (stress asymmetry ratio R = 0, frequency f = 20 Hz). Additionally, 4 of the tests, characterized by the highest amplitude, were accompanied with non-contact strain field measurements by means of electronic speckle pattern interferometry and digital image correlation. Results of these measurements allowed to localize the areas of deformation concentration identified as the damage points of the surface layer or advanced crack presence in core material. Identification and observation of the development of deformation in localization areas allowed to assess fatigue-related phenomena in both layer and substrate materials.

Adaptive DSPI phase denoising using mutual information and 2D variational mode decomposition

NASA Astrophysics Data System (ADS)

Xiao, Qiyang; Li, Jian; Wu, Sijin; Li, Weixian; Yang, Lianxiang; Dong, Mingli; Zeng, Zhoumo

2018-04-01

In digital speckle pattern interferometry (DSPI), noise interference leads to a low peak signal-to-noise ratio (PSNR) and measurement errors in the phase map. This paper proposes an adaptive DSPI phase denoising method based on two-dimensional variational mode decomposition (2D-VMD) and mutual information. Firstly, the DSPI phase map is subjected to 2D-VMD in order to obtain a series of band-limited intrinsic mode functions (BLIMFs). Then, on the basis of characteristics of the BLIMFs and in combination with mutual information, a self-adaptive denoising method is proposed to obtain noise-free components containing the primary phase information. The noise-free components are reconstructed to obtain the denoising DSPI phase map. Simulation and experimental results show that the proposed method can effectively reduce noise interference, giving a PSNR that is higher than that of two-dimensional empirical mode decomposition methods.

Effect of ambiguities on SAR picture quality

NASA Technical Reports Server (NTRS)

Korwar, V. N.; Lipes, R. G.

1978-01-01

The degradation of picture quality is studied for a high-resolution, large-swath SAR mapping system subjected to speckle, additive white Gaussian noise, and range and azimuthal ambiguities occurring because of the non-finite antenna pattern produced by a square aperture antenna. The effect of the azimuth antenna pattern was accounted for by calculating the aximuth ambiguity function. Range ambiguities were accounted for by adding appropriate pixels at a range separation corresponding to one pulse repetition period, but attenuated by the antenna pattern. A method of estimating the range defocussing effect which arises from the azimuth matched filter being a function of range is shown. The resulting simulated picture was compared with one degraded by speckle and noise but no ambiguities. It is concluded that azimuth ambiguities don't cause any noticeable degradation but range ambiguities might.

Enhanced tagging of light utilizing acoustic radiation force with speckle pattern analysis

NASA Astrophysics Data System (ADS)

Vakili, Ali; Hollmann, Joseph L.; Holt, R. Glynn; DiMarzio, Charles A.

2017-10-01

In optical imaging, the depth and resolution are limited due to scattering. Unlike light, scattering of ultrasound (US) waves in tissue is negligible. Hybrid imaging methods such as US-modulated optical tomography (UOT) use the advantages of both modalities. UOT tags light by inducing phase change caused by modulating the local index of refraction of the medium. The challenge in UOT is detecting the small signal. The displacement induced by the acoustic radiation force (ARF) is another US effect that can be utilized to tag the light. It induces greater phase change, resulting in a stronger signal. Moreover, the absorbed acoustic energy generates heat, resulting in change in the index of refraction and a strong phase change. The speckle pattern is governed by the phase of the interfering scattered waves; hence, speckle pattern analysis can obtain information about displacement and temperature changes. We have presented a model to simulate the insonation processes. Simulation results based on fixed-particle Monte Carlo and experimental results show that the signal acquired by utilizing ARF is stronger compared to UOT. The introduced mean irradiance change (MIC) signal reveals both thermal and mechanical effects of the focused US beam in different timescales. Simulation results suggest that variation in the MIC signal can be used to generate a displacement image of the medium.

Soft tissue strain measurement using an optical method

NASA Astrophysics Data System (ADS)

Toh, Siew Lok; Tay, Cho Jui; Goh, Cho Hong James

2008-11-01

Digital image correlation (DIC) is a non-contact optical technique that allows the full-field estimation of strains on a surface under an applied deformation. In this project, the application of an optimized DIC technique is applied, which can achieve efficiency and accuracy in the measurement of two-dimensional deformation fields in soft tissue. This technique relies on matching the random patterns recorded in images to directly obtain surface displacements and to get displacement gradients from which the strain field can be determined. Digital image correlation is a well developed technique that has numerous and varied engineering applications, including the application in soft and hard tissue biomechanics. Chicken drumstick ligaments were harvested and used during the experiments. The surface of the ligament was speckled with black paint to allow for correlation to be done. Results show that the stress-strain curve exhibits a bi-linear behavior i.e. a "toe region" and a "linear elastic region". The Young's modulus obtained for the toe region is about 92 MPa and the modulus for the linear elastic region is about 230 MPa. The results are within the values for mammalian anterior cruciate ligaments of 150-300 MPa.

[Glossary of terms used by radiologists in image processing].

PubMed

Rolland, Y; Collorec, R; Bruno, A; Ramée, A; Morcet, N; Haigron, P

1995-01-01

We give the definition of 166 words used in image processing. Adaptivity, aliazing, analog-digital converter, analysis, approximation, arc, artifact, artificial intelligence, attribute, autocorrelation, bandwidth, boundary, brightness, calibration, class, classification, classify, centre, cluster, coding, color, compression, contrast, connectivity, convolution, correlation, data base, decision, decomposition, deconvolution, deduction, descriptor, detection, digitization, dilation, discontinuity, discretization, discrimination, disparity, display, distance, distorsion, distribution dynamic, edge, energy, enhancement, entropy, erosion, estimation, event, extrapolation, feature, file, filter, filter floaters, fitting, Fourier transform, frequency, fusion, fuzzy, Gaussian, gradient, graph, gray level, group, growing, histogram, Hough transform, Houndsfield, image, impulse response, inertia, intensity, interpolation, interpretation, invariance, isotropy, iterative, JPEG, knowledge base, label, laplacian, learning, least squares, likelihood, matching, Markov field, mask, matching, mathematical morphology, merge (to), MIP, median, minimization, model, moiré, moment, MPEG, neural network, neuron, node, noise, norm, normal, operator, optical system, optimization, orthogonal, parametric, pattern recognition, periodicity, photometry, pixel, polygon, polynomial, prediction, pulsation, pyramidal, quantization, raster, reconstruction, recursive, region, rendering, representation space, resolution, restoration, robustness, ROC, thinning, transform, sampling, saturation, scene analysis, segmentation, separable function, sequential, smoothing, spline, split (to), shape, threshold, tree, signal, speckle, spectrum, spline, stationarity, statistical, stochastic, structuring element, support, syntaxic, synthesis, texture, truncation, variance, vision, voxel, windowing.

In vivo lateral blood flow velocity measurement using speckle size estimation.

PubMed

Xu, Tiantian; Hozan, Mohsen; Bashford, Gregory R

2014-05-01

In previous studies, we proposed blood measurement using speckle size estimation, which estimates the lateral component of blood flow within a single image frame based on the observation that the speckle pattern corresponding to blood reflectors (typically red blood cells) stretches (i.e., is "smeared") if blood flow is in the same direction as the electronically controlled transducer line selection in a 2-D image. In this observational study, the clinical viability of ultrasound blood flow velocity measurement using speckle size estimation was investigated and compared with that of conventional spectral Doppler of carotid artery blood flow data collected from human patients in vivo. Ten patients (six male, four female) were recruited. Right carotid artery blood flow data were collected in an interleaved fashion (alternating Doppler and B-mode A-lines) with an Antares Ultrasound Imaging System and transferred to a PC via the Axius Ultrasound Research Interface. The scanning velocity was 77 cm/s, and a 4-s interval of flow data were collected from each subject to cover three to five complete cardiac cycles. Conventional spectral Doppler data were collected simultaneously to compare with estimates made by speckle size estimation. The results indicate that the peak systolic velocities measured with the two methods are comparable (within ±10%) if the scan velocity is greater than or equal to the flow velocity. When scan velocity is slower than peak systolic velocity, the speckle stretch method asymptotes to the scan velocity. Thus, the speckle stretch method is able to accurately measure pure lateral flow, which conventional Doppler cannot do. In addition, an initial comparison of the speckle size estimation and color Doppler methods with respect to computational complexity and data acquisition time indicated potential time savings in blood flow velocity estimation using speckle size estimation. Further studies are needed for calculation of the speckle stretch method across a field of view and combination with an appropriate axial flow estimator. Copyright © 2014 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Multi-wavelength speckle reduction for laser pico-projectors using diffractive optics

NASA Astrophysics Data System (ADS)

Thomas, Weston H.

Personal electronic devices, such as cell phones and tablets, continue to decrease in size while the number of features and add-ons keep increasing. One particular feature of great interest is an integrated projector system. Laser pico-projectors have been considered, but the technology has not been developed enough to warrant integration. With new advancements in diode technology and MEMS devices, laser-based projection is currently being advanced for pico-projectors. A primary problem encountered when using a pico-projector is coherent interference known as speckle. Laser speckle can lead to eye irritation and headaches after prolonged viewing. Diffractive optical elements known as diffusers have been examined as a means to lower speckle contrast. Diffusers are often rotated to achieve temporal averaging of the spatial phase pattern provided by diffuser surface. While diffusers are unable to completely eliminate speckle, they can be utilized to decrease the resultant contrast to provide a more visually acceptable image. This dissertation measures the reduction in speckle contrast achievable through the use of diffractive diffusers. A theoretical Fourier optics model is used to provide the diffuser's stationary and in-motion performance in terms of the resultant contrast level. Contrast measurements of two diffractive diffusers are calculated theoretically and compared with experimental results. In addition, a novel binary diffuser design based on Hadamard matrices will be presented. Using two static in-line Hadamard diffusers eliminates the need for rotation or vibration of the diffuser for temporal averaging. Two Hadamard diffusers were fabricated and contrast values were subsequently measured, showing good agreement with theory and simulated values. Monochromatic speckle contrast values of 0.40 were achieved using the Hadamard diffusers. Finally, color laser projection devices require the use of red, green, and blue laser sources; therefore, using a monochromatic diffractive diffuser may not optimal for color speckle contrast reduction. A simulation of the Hadamard diffusers is conducted to determine the optimum spacing between the two diffusers for polychromatic speckle reduction. Experimental measured results are presented using the optimal spacing of Hadamard diffusers for RGB color speckle reduction, showing 60% reduction in contrast.

Techniques of noninvasive optical tomographic imaging

NASA Astrophysics Data System (ADS)

Rosen, Joseph; Abookasis, David; Gokhler, Mark

2006-01-01

Recently invented methods of optical tomographic imaging through scattering and absorbing media are presented. In one method, the three-dimensional structure of an object hidden between two biological tissues is recovered from many noisy speckle pictures obtained on the output of a multi-channeled optical imaging system. Objects are recovered from many speckled images observed by a digital camera through two stereoscopic microlens arrays. Each microlens in each array generates a speckle image of the object buried between the layers. In the computer each image is Fourier transformed jointly with an image of the speckled point-like source captured under the same conditions. A set of the squared magnitudes of the Fourier-transformed pictures is accumulated to form a single average picture. This final picture is again Fourier transformed, resulting in the three-dimensional reconstruction of the hidden object. In the other method, the effect of spatial longitudinal coherence is used for imaging through an absorbing layer with different thickness, or different index of refraction, along the layer. The technique is based on synthesis of multiple peak spatial degree of coherence. This degree of coherence enables us to scan simultaneously different sample points on different altitudes, and thus decreases the acquisition time. The same multi peak degree of coherence is also used for imaging through the absorbing layer. Our entire experiments are performed with a quasi-monochromatic light source. Therefore problems of dispersion and inhomogeneous absorption are avoided.

Developments In Electronic Speckle Pattern Interferometry For Automotive Vibration Analysis.

NASA Astrophysics Data System (ADS)

Davies, Jeremy C.; Buckberry, Clive H.; Jones, Julian D. C.; Pannell, Chris N.

1989-01-01

The incorporation of monomode fibre optics into an argon ion powered Electronic Speckle Pattern Interferometer (ESPI) is reported. The system, consisting of an optics assembly linked to the laser and a CCD camera transceiver, flexibly connected by 40m of monomode fibre optic cable to the optics, has been used to analyse the modal behaviour of structures up to 5m X 3m X 2m in size. Phase modulation of the reference beam in order to operate in a heterodyne mode has been implemented using a piezo-electric crystal operating on the monomode fibre. A new mode of operation - sequential time-average subtraction - and the results of a new processing algorithm are also reported. Their implementation enables speckle free, time-average vibration maps to be generated in real-time on large, unstable structures. Example results for a four cylinder power unit, a vehicle body shell component and an engine oil pan are included. In all cases the analysis was conducted in a general workshop environment without the need for vibration isolation facilities.

FBI-1 can stimulate HIV-1 Tat activity and is targeted to a novel subnuclear domain that includes the Tat-P-TEFb-containing nuclear speckles.

PubMed

Pendergrast, P Shannon; Wang, Chen; Hernandez, Nouria; Huang, Sui

2002-03-01

FBI-1 is a cellular POZ-domain-containing protein that binds to the HIV-1 LTR and associates with the HIV-1 transactivator protein Tat. Here we show that elevated levels of FBI-1 specifically stimulate Tat activity and that this effect is dependent on the same domain of FBI-1 that mediates Tat-FBI-1 association in vivo. FBI-1 also partially colocalizes with Tat and Tat's cellular cofactor, P-TEFb (Cdk9 and cyclin T1), at the splicing-factor-rich nuclear speckle domain. Further, a less-soluble population of FBI-1 distributes in a novel peripheral-speckle pattern of localization as well as in other nuclear regions. This distribution pattern is dependent on the FBI-1 DNA binding domain, on the presence of cellular DNA, and on active transcription. Taken together, these results suggest that FBI-1 is a cellular factor that preferentially associates with active chromatin and that can specifically stimulate Tat-activated HIV-1 transcription.

FBI-1 Can Stimulate HIV-1 Tat Activity and Is Targeted to a Novel Subnuclear Domain that Includes the Tat-P-TEFb—containing Nuclear Speckles

PubMed Central

Pendergrast, P. Shannon; Wang, Chen; Hernandez, Nouria; Huang, Sui

2002-01-01

FBI-1 is a cellular POZ-domain–containing protein that binds to the HIV-1 LTR and associates with the HIV-1 transactivator protein Tat. Here we show that elevated levels of FBI-1 specifically stimulate Tat activity and that this effect is dependent on the same domain of FBI-1 that mediates Tat-FBI-1 association in vivo. FBI-1 also partially colocalizes with Tat and Tat's cellular cofactor, P-TEFb (Cdk9 and cyclin T1), at the splicing-factor–rich nuclear speckle domain. Further, a less-soluble population of FBI-1 distributes in a novel peripheral-speckle pattern of localization as well as in other nuclear regions. This distribution pattern is dependent on the FBI-1 DNA binding domain, on the presence of cellular DNA, and on active transcription. Taken together, these results suggest that FBI-1 is a cellular factor that preferentially associates with active chromatin and that can specifically stimulate Tat-activated HIV-1 transcription. PMID:11907272

Optical encryption of digital data in form of quick response code using spatially incoherent illumination

NASA Astrophysics Data System (ADS)

Cheremkhin, Pavel A.; Krasnov, Vitaly V.; Rodin, Vladislav G.; Starikov, Rostislav S.

2016-11-01

Applications of optical methods for encryption purposes have been attracting interest of researchers for decades. The most popular are coherent techniques such as double random phase encoding. Its main advantage is high security due to transformation of spectrum of image to be encrypted into white spectrum via use of first phase random mask which allows for encrypted images with white spectra. Downsides are necessity of using holographic registration scheme and speckle noise occurring due to coherent illumination. Elimination of these disadvantages is possible via usage of incoherent illumination. In this case, phase registration no longer matters, which means that there is no need for holographic setup, and speckle noise is gone. Recently, encryption of digital information in form of binary images has become quite popular. Advantages of using quick response (QR) code in capacity of data container for optical encryption include: 1) any data represented as QR code will have close to white (excluding zero spatial frequency) Fourier spectrum which have good overlapping with encryption key spectrum; 2) built-in algorithm for image scale and orientation correction which simplifies decoding of decrypted QR codes; 3) embedded error correction code allows for successful decryption of information even in case of partial corruption of decrypted image. Optical encryption of digital data in form QR codes using spatially incoherent illumination was experimentally implemented. Two liquid crystal spatial light modulators were used in experimental setup for QR code and encrypting kinoform imaging respectively. Decryption was conducted digitally. Successful decryption of encrypted QR codes is demonstrated.

2D biological representations with reduced speckle obtained from two perpendicular ultrasonic arrays.

PubMed

Rodriguez-Hernandez, Miguel A; Gomez-Sacristan, Angel; Sempere-Payá, Víctor M

2016-04-29

Ultrasound diagnosis is a widely used medical tool. Among the various ultrasound techniques, ultrasonic imaging is particularly relevant. This paper presents an improvement to a two-dimensional (2D) ultrasonic system using measurements taken from perpendicular planes, where digital signal processing techniques are used to combine one-dimensional (1D) A-scans were acquired by individual transducers in arrays located in perpendicular planes. An algorithm used to combine measurements is improved based on the wavelet transform, which includes a denoising step during the 2D representation generation process. The inclusion of this new denoising stage generates higher quality 2D representations with a reduced level of speckling. The paper includes different 2D representations obtained from noisy A-scans and compares the improvements obtained by including the denoising stage.

Sensitivity evaluation of dynamic speckle activity measurements using clustering methods.

PubMed

Etchepareborda, Pablo; Federico, Alejandro; Kaufmann, Guillermo H

2010-07-01

We evaluate and compare the use of competitive neural networks, self-organizing maps, the expectation-maximization algorithm, K-means, and fuzzy C-means techniques as partitional clustering methods, when the sensitivity of the activity measurement of dynamic speckle images needs to be improved. The temporal history of the acquired intensity generated by each pixel is analyzed in a wavelet decomposition framework, and it is shown that the mean energy of its corresponding wavelet coefficients provides a suited feature space for clustering purposes. The sensitivity obtained by using the evaluated clustering techniques is also compared with the well-known methods of Konishi-Fujii, weighted generalized differences, and wavelet entropy. The performance of the partitional clustering approach is evaluated using simulated dynamic speckle patterns and also experimental data.

Noncontact speckle-based optical sensor for detection of glucose concentration using magneto-optic effect

NASA Astrophysics Data System (ADS)

Ozana, Nisan; Beiderman, Yevgeny; Anand, Arun; Javidi, Baharam; Polani, Sagi; Schwarz, Ariel; Shemer, Amir; Garcia, Javier; Zalevsky, Zeev

2016-06-01

We experimentally verify a speckle-based technique for noncontact measurement of glucose concentration in the bloodstream. The final device is intended to be a single wristwatch-style device containing a laser, a camera, and an alternating current (ac) electromagnet generated by a solenoid. The experiments presented are performed in vitro as proof of the concept. When a glucose substance is inserted into a solenoid generating an ac magnetic field, it exhibits Faraday rotation, which affects the temporal changes of the secondary speckle pattern distributions. The temporal frequency resulting from the ac magnetic field was found to have a lock-in amplification role, which increased the observability of the relatively small magneto-optic effect. Experimental results to support the proposed concept are presented.

Simultaneously extracting multiple parameters via multi-distance and multi-exposure diffuse speckle contrast analysis

PubMed Central

Liu, Jialin; Zhang, Hongchao; Lu, Jian; Ni, Xiaowu; Shen, Zhonghua

2017-01-01

Recent advancements in diffuse speckle contrast analysis (DSCA) have opened the path for noninvasive acquisition of deep tissue microvasculature blood flow. In fact, in addition to blood flow index αDB, the variations of tissue optical absorption μa, reduced scattering coefficients μs′, as well as coherence factor β can modulate temporal fluctuations of speckle patterns. In this study, we use multi-distance and multi-exposure DSCA (MDME-DSCA) to simultaneously extract multiple parameters such as μa, μs′, αDB, and β. The validity of MDME-DSCA has been validated by the simulated data and phantoms experiments. Moreover, as a comparison, the results also show that it is impractical to simultaneously obtain multiple parameters by multi-exposure DSCA (ME-DSCA). PMID:29082083

Experimental Observation of Near-Field Deterioration Induced by Stimulated Rotational Raman Scattering in Long Air Paths

NASA Astrophysics Data System (ADS)

Wang, Jing; Zhang, Xiao-Min; Han, Wei; Li, Fu-Quan; Zhou, Li-Dan; Feng, Bin; Xiang, Yong

2011-08-01

We report the experimental investigation of a stimulated rotational Raman scattering effect in long air paths on SG-III TIL, with a 1053 nm, 20-cm-diameter, linearly polarized, 3 ns flat-topped laser pulse. An intense speckle pattern of near field with thickly dotted hot spots is observed at the end of propagation with an intensity-length product above 17TW/cm. The Stokes developing from the scattering of the laser beam by quantum fluctuations is characterized by a combination of high spatial frequency components. The observed speckle pattern with small-diameter hot spots results from the combination of the nonlinear Raman amplification and the linear diffraction propagation effect of the Stokes with a noise pattern arising from the spontaneous Raman scattering. A new promising suppression concept based on the special characteristic of the Stokes, called active and selective filtering of Stokes, is proposed.

Coherent and incoherent imaging through scattering media (Conference Presentation)

NASA Astrophysics Data System (ADS)

Edrei, Eitan

2017-02-01

The shower-curtain effect is a familiar phenomenon, routinely observed in our everyday life: an object placed behind a scattering layer appears blurred but if the object is attached to the scattering layer it can be clearly resolved. The optical system we developed takes advantage of the shower-curtain effect properties and generalizes them to achieve high-resolution imaging of objects placed at a nearly arbitrary distance behind the scattering medium. The imaging procedure is based on retrieving the object Fourier transform from the turbid medium (used as the shower-curtain) through a correlography technique based on speckle illumination. Illuminating the object with a speckle pattern rather than a coherent beam, we show that the correlography principles can be effectively applied in the near field. While the far-field condition is usually known as z<(2D^2)⁄λ (D, size of the object; λ wavelength); by tuning the spatial coherence of the illumination beam, as one can do with speckle illumination, the "far-field" condition can be written as z<(2DRc)⁄λ where Rc is the correlation radius of the speckle pattern. Using our method we present high-resolution imaging of objects hidden behind millimeter-thick tissue or dense lens cataracts, and demonstrate our imaging technique to be insensitive to rapid medium movements (<5 m/s) beyond any biologically relevant motion. Furthermore, we show this method can be extended to several contrast mechanisms and imaging configurations.

Differentiation of the drying time of adhesives on plywoods through the dynamic speckle technique

NASA Astrophysics Data System (ADS)

Kumari, S.; Nirala, A. K.

2018-02-01

The drying time of adhesives such as Fevicol SH, Fevicol MR, Dendrite white and Bulbond after coating separately on the three plywoods, namely Archidply, Centuryply and Greenply, has been studied non-destructively using the dynamic speckle technique. The time history of the speckle pattern, the co-occurrence matrix, 3D graphs and line profiles of images from the time history of the speckle pattern along with 3D trajectory plots have been used for qualitative analysis whereas the inertia moment, absolute value difference, SM index and autocovariance have been used for quantitative analysis. The gray-level co-occurrence matrix has been used for the first time to study the textual parameters of adhesive coated on plywoods during drying. The average drying time of adhesive is a maximum for Bulbond on Archidply (357.25  ±  1.49 min) and a minimum for Dendrite white on Greenply (90.75  ±  2.36 min). Comparative studies among the results obtained for all the four adhesives on the three plywoods reveal that Dendrite white adhesive is the best among the adhesives because it takes the shortest time to dry on all the plywoods, and Greenply is the best among all the plywoods because drying is fastest on it for all the adhesives. Furthermore, it is also concluded that the best plywood and the best adhesive may be decided by knowing the remnant activity.

A novel speckle pattern—Adaptive digital image correlation approach with robust strain calculation

NASA Astrophysics Data System (ADS)

Cofaru, Corneliu; Philips, Wilfried; Van Paepegem, Wim

2012-02-01

Digital image correlation (DIC) has seen widespread acceptance and usage as a non-contact method for the determination of full-field displacements and strains in experimental mechanics. The advances of imaging hardware in the last decades led to high resolution and speed cameras being more affordable than in the past making large amounts of data image available for typical DIC experimental scenarios. The work presented in this paper is aimed at maximizing both the accuracy and speed of DIC methods when employed with such images. A low-level framework for speckle image partitioning which replaces regularly shaped blocks with image-adaptive cells in the displacement calculation is introduced. The Newton-Raphson DIC method is modified to use the image pixels of the cells and to perform adaptive regularization to increase the spatial consistency of the displacements. Furthermore, a novel robust framework for strain calculation based also on the Newton-Raphson algorithm is introduced. The proposed methods are evaluated in five experimental scenarios, out of which four use numerically deformed images and one uses real experimental data. Results indicate that, as the desired strain density increases, significant computational gains can be obtained while maintaining or improving accuracy and rigid-body rotation sensitivity.

Digital Mirror Device Application in Reduction of Wave-front Phase Errors

PubMed Central

Zhang, Yaping; Liu, Yan; Wang, Shuxue

2009-01-01

In order to correct the image distortion created by the mixing/shear layer, creative and effectual correction methods are necessary. First, a method combining adaptive optics (AO) correction with a digital micro-mirror device (DMD) is presented. Second, performance of an AO system using the Phase Diverse Speckle (PDS) principle is characterized in detail. Through combining the DMD method with PDS, a significant reduction in wavefront phase error is achieved in simulations and experiments. This kind of complex correction principle can be used to recovery the degraded images caused by unforeseen error sources. PMID:22574016

Learnable despeckling framework for optical coherence tomography images

NASA Astrophysics Data System (ADS)

Adabi, Saba; Rashedi, Elaheh; Clayton, Anne; Mohebbi-Kalkhoran, Hamed; Chen, Xue-wen; Conforto, Silvia; Nasiriavanaki, Mohammadreza

2018-01-01

Optical coherence tomography (OCT) is a prevalent, interferometric, high-resolution imaging method with broad biomedical applications. Nonetheless, OCT images suffer from an artifact called speckle, which degrades the image quality. Digital filters offer an opportunity for image improvement in clinical OCT devices, where hardware modification to enhance images is expensive. To reduce speckle, a wide variety of digital filters have been proposed; selecting the most appropriate filter for an OCT image/image set is a challenging decision, especially in dermatology applications of OCT where a different variety of tissues are imaged. To tackle this challenge, we propose an expandable learnable despeckling framework, we call LDF. LDF decides which speckle reduction algorithm is most effective on a given image by learning a figure of merit (FOM) as a single quantitative image assessment measure. LDF is learnable, which means when implemented on an OCT machine, each given image/image set is retrained and its performance is improved. Also, LDF is expandable, meaning that any despeckling algorithm can easily be added to it. The architecture of LDF includes two main parts: (i) an autoencoder neural network and (ii) filter classifier. The autoencoder learns the FOM based on several quality assessment measures obtained from the OCT image including signal-to-noise ratio, contrast-to-noise ratio, equivalent number of looks, edge preservation index, and mean structural similarity index. Subsequently, the filter classifier identifies the most efficient filter from the following categories: (a) sliding window filters including median, mean, and symmetric nearest neighborhood, (b) adaptive statistical-based filters including Wiener, homomorphic Lee, and Kuwahara, and (c) edge preserved patch or pixel correlation-based filters including nonlocal mean, total variation, and block matching three-dimensional filtering.

Multiscale deformation behavior for multilayered steel by in-situ FE-SEM

NASA Astrophysics Data System (ADS)

Tanaka, Y.; Kishimoto, S.; Yin, F.; Kobayashi, M.; Tomimatsu, T.; Kagawa, K.

2010-03-01

The multi-scale deformation behavior of multi-layered steel during tensile loading was investigated by in-situ FE-SEM observation coupled with multi-scale pattern. The material used was multi-layered steel sheet consisting of martensitic and austenitic stainless steel layers. Prior to in-situ tensile testing, the multi-scale pattern combined with a grid and random dots were fabricated by electron beam lithography on the polished surface in the area of 1 mm2 to facilitate direct observation of multi-scale deformation. Both of the grids with pitches of 10 μm and a random speckle pattern ranging from 200 nm to a few μm sizes were drawn onto the specimen surface at same location. The electron moiré method was applied to measure the strain distribution in the deformed specimens at a millimeter scale and digital images correlation method was applied to measure the in-plane deformation and strain distribution at a micron meter scale acquired before and after at various increments of straining. The results showed that the plastic deformation in the austenitic stainless steel layer was larger than the martensitic steel layer at millimeter scale. However, heterogeneous intrinsic grain-scale plastic deformation was clearly observed and it increased with increasing the plastic deformation.

Local scattering property scales flow speed estimation in laser speckle contrast imaging

NASA Astrophysics Data System (ADS)

Miao, Peng; Chao, Zhen; Feng, Shihan; Yu, Hang; Ji, Yuanyuan; Li, Nan; Thakor, Nitish V.

2015-07-01

Laser speckle contrast imaging (LSCI) has been widely used in in vivo blood flow imaging. However, the effect of local scattering property (scattering coefficient µ s ) on blood flow speed estimation has not been well investigated. In this study, such an effect was quantified and involved in relation between speckle autocorrelation time τ c and flow speed v based on simulation flow experiments. For in vivo blood flow imaging, an improved estimation strategy was developed to eliminate the estimation bias due to the inhomogeneous distribution of the scattering property. Compared to traditional LSCI, a new estimation method significantly suppressed the imaging noise and improves the imaging contrast of vasculatures. Furthermore, the new method successfully captured the blood flow changes and vascular constriction patterns in rats’ cerebral cortex from normothermia to mild and moderate hypothermia.

Optics in engineering measurement; Proceedings of the Meeting, Cannes, France, December 3-6, 1985

NASA Technical Reports Server (NTRS)

Fagan, William F. (Editor)

1986-01-01

The present conference on optical measurement systems considers topics in the fields of holographic interferometry, speckle techniques, moire fringe and grating methods, optical surface gaging, laser- and fiber-optics-based measurement systems, and optics for engineering data evaluation. Specific attention is given to holographic NDE for aerospace composites, holographic interferometry of rotating components, new developments in computer-aided holography, electronic speckle pattern interferometry, mass transfer measurements using projected fringes, nuclear reactor photogrammetric inspection, a laser Doppler vibrometer, and optoelectronic measurements of the yaw angle of projectiles.

X-ray phase contrast tomography by tracking near field speckle

PubMed Central

Wang, Hongchang; Berujon, Sebastien; Herzen, Julia; Atwood, Robert; Laundy, David; Hipp, Alexander; Sawhney, Kawal

2015-01-01

X-ray imaging techniques that capture variations in the x-ray phase can yield higher contrast images with lower x-ray dose than is possible with conventional absorption radiography. However, the extraction of phase information is often more difficult than the extraction of absorption information and requires a more sophisticated experimental arrangement. We here report a method for three-dimensional (3D) X-ray phase contrast computed tomography (CT) which gives quantitative volumetric information on the real part of the refractive index. The method is based on the recently developed X-ray speckle tracking technique in which the displacement of near field speckle is tracked using a digital image correlation algorithm. In addition to differential phase contrast projection images, the method allows the dark-field images to be simultaneously extracted. After reconstruction, compared to conventional absorption CT images, the 3D phase CT images show greatly enhanced contrast. This new imaging method has advantages compared to other X-ray imaging methods in simplicity of experimental arrangement, speed of measurement and relative insensitivity to beam movements. These features make the technique an attractive candidate for material imaging such as in-vivo imaging of biological systems containing soft tissue. PMID:25735237

Optical characterization of display screens by speckle patterns

NASA Astrophysics Data System (ADS)

Pozo, Antonio M.; Castro, José J.; Rubiño, Manuel

2013-10-01

In recent years, flat-panel display (FPD) technology has undergone great development, and now FPDs appear in many devices. A significant element in FPD manufacturing is the display front surface. Manufacturers sell FPDs with different types of front surfaces, which can be matte (also called anti-glare) or glossy screens. Users who prefer glossy screens consider these displays to show more vivid colors compared with matte-screen displays. However, on the glossy screens, external light sources may cause unpleasant reflections that can be reduced by a matte treatment in the front surface. In this work, we present a method to characterize FPD screens using laser-speckle patterns. We characterize three FPDs: a Samsung XL2370 LCD monitor of 23 in. with matte screen, a Toshiba Satellite A100 LCD laptop of 15.4 in. with glossy screen, and a Grammata Papyre 6.1 electronic book reader of 6 in. with ePaper screen (E-ink technology). The results show great differences in speckle-contrast values for the three screens characterized and, therefore, this work shows the feasibility of this method for characterizing and comparing FPDs that have different types of front surfaces.

Image analysis of speckle patterns as a probe of melting transitions in laser-heated diamond anvil cell experiments.

PubMed

Salem, Ran; Matityahu, Shlomi; Melchior, Aviva; Nikolaevsky, Mark; Noked, Ori; Sterer, Eran

2015-09-01

The precision of melting curve measurements using laser-heated diamond anvil cell (LHDAC) is largely limited by the correct and reliable determination of the onset of melting. We present a novel image analysis of speckle interference patterns in the LHDAC as a way to define quantitative measures which enable an objective determination of the melting transition. Combined with our low-temperature customized IR pyrometer, designed for measurements down to 500 K, our setup allows studying the melting curve of materials with low melting temperatures, with relatively high precision. As an application, the melting curve of Te was measured up to 35 GPa. The results are found to be in good agreement with previous data obtained at pressures up to 10 GPa.

Breast cancer early detection via tracking of skin back-scattered secondary speckle patterns

NASA Astrophysics Data System (ADS)

Bennett, Aviya; Sirkis, Talia; Beiderman, Yevgeny; Agdarov, Sergey; Beiderman, Yafim; Zalevsky, Zeev

2018-02-01

Breast cancer has become a major cause of death among women. The lifetime risk of a woman developing this disease has been established as one in eight. The most useful way to reduce breast cancer death is to treat the disease as early as possible. The existing methods of early diagnostics of breast cancer are mainly based on screening mammography or Magnetic Resonance Imaging (MRI) periodically conducted at medical facilities. In this paper the authors proposing a new approach for simple breast cancer detection. It is based on skin stimulation by sound waves, illuminating it by laser beam and tracking the reflected secondary speckle patterns. As first approach, plastic balls of different sizes were placed under the skin of chicken breast and detected by the proposed method.

Dictionary learning-based spatiotemporal regularization for 3D dense speckle tracking

NASA Astrophysics Data System (ADS)

Lu, Allen; Zontak, Maria; Parajuli, Nripesh; Stendahl, John C.; Boutagy, Nabil; Eberle, Melissa; O'Donnell, Matthew; Sinusas, Albert J.; Duncan, James S.

2017-03-01

Speckle tracking is a common method for non-rigid tissue motion analysis in 3D echocardiography, where unique texture patterns are tracked through the cardiac cycle. However, poor tracking often occurs due to inherent ultrasound issues, such as image artifacts and speckle decorrelation; thus regularization is required. Various methods, such as optical flow, elastic registration, and block matching techniques have been proposed to track speckle motion. Such methods typically apply spatial and temporal regularization in a separate manner. In this paper, we propose a joint spatiotemporal regularization method based on an adaptive dictionary representation of the dense 3D+time Lagrangian motion field. Sparse dictionaries have good signal adaptive and noise-reduction properties; however, they are prone to quantization errors. Our method takes advantage of the desirable noise suppression, while avoiding the undesirable quantization error. The idea is to enforce regularization only on the poorly tracked trajectories. Specifically, our method 1.) builds data-driven 4-dimensional dictionary of Lagrangian displacements using sparse learning, 2.) automatically identifies poorly tracked trajectories (outliers) based on sparse reconstruction errors, and 3.) performs sparse reconstruction of the outliers only. Our approach can be applied on dense Lagrangian motion fields calculated by any method. We demonstrate the effectiveness of our approach on a baseline block matching speckle tracking and evaluate performance of the proposed algorithm using tracking and strain accuracy analysis.

Yeast Formins Bni1 and Bnr1 Utilize Different Modes of Cortical Interaction during the Assembly of Actin Cables

PubMed Central

Buttery, Shawnna M.; Yoshida, Satoshi

2007-01-01

The budding yeast formins Bni1 and Bnr1 control the assembly of actin cables. These formins exhibit distinct patterns of localization and polymerize two different populations of cables: Bni1 in the bud and Bnr1 in the mother cell. We generated a functional Bni1-3GFP that improved the visualization of Bni1 in vivo at endogenous levels. Bni1 exists as speckles in the cytoplasm, some of which colocalize on actin cables. These Bni1 speckles display linear, retrograde-directed movements. Loss of polymerized actin or specifically actin cables abolished retrograde movement, and resulted in depletion of Bni1 speckles from the cytoplasm, with enhanced targeting of Bni1 to the bud tip. Mutations that impair the actin assembly activity of Bni1 abolished the movement of Bni1 speckles, even when actin cables were present. In contrast, Bnr1-GFP or 3GFP-Bnr1 did not detectably associate with actin cables and was not observed as cytoplasmic speckles. Finally, fluorescence recovery after photobleaching demonstrated that Bni1 was very dynamic, exchanging between polarized sites and the cytoplasm, whereas Bnr1 was confined to the bud neck and did not exchange with a cytoplasmic pool. In summary, our results indicate that formins can have distinct modes of cortical interaction during actin cable assembly. PMID:17344480

Yeast formins Bni1 and Bnr1 utilize different modes of cortical interaction during the assembly of actin cables.

PubMed

Buttery, Shawnna M; Yoshida, Satoshi; Pellman, David

2007-05-01

The budding yeast formins Bni1 and Bnr1 control the assembly of actin cables. These formins exhibit distinct patterns of localization and polymerize two different populations of cables: Bni1 in the bud and Bnr1 in the mother cell. We generated a functional Bni1-3GFP that improved the visualization of Bni1 in vivo at endogenous levels. Bni1 exists as speckles in the cytoplasm, some of which colocalize on actin cables. These Bni1 speckles display linear, retrograde-directed movements. Loss of polymerized actin or specifically actin cables abolished retrograde movement, and resulted in depletion of Bni1 speckles from the cytoplasm, with enhanced targeting of Bni1 to the bud tip. Mutations that impair the actin assembly activity of Bni1 abolished the movement of Bni1 speckles, even when actin cables were present. In contrast, Bnr1-GFP or 3GFP-Bnr1 did not detectably associate with actin cables and was not observed as cytoplasmic speckles. Finally, fluorescence recovery after photobleaching demonstrated that Bni1 was very dynamic, exchanging between polarized sites and the cytoplasm, whereas Bnr1 was confined to the bud neck and did not exchange with a cytoplasmic pool. In summary, our results indicate that formins can have distinct modes of cortical interaction during actin cable assembly.

Mechanical characterization of natural building stones: observation of the fracture process zone by ESPI

NASA Astrophysics Data System (ADS)

Calvetti, Francesco; Cardani, Giuliana; Meda, Alberto

1999-09-01

The cultural heritage of many nations consist of a great variety of structures of high intrinsic value, which are often composed of natural building stones (NBS), as granite, limestone, marble and sandstone. The use of accurate inspection devices, such as laser interferometry, allows us to acquire information regarding the mechanical properties and damage (tensile cracks) of NBS, which represents the first step in the restoration process. In this paper, the potential application of an electronic speckle pattern interferometry (ESPI) is shown, with particular attention to the observed displacement field and the crack penetration during laboratory testing. In ESPI, by superimposing a reflected light to a reference digitized image, an interference phenomenon is produced. By comparing two recorded interference patterns (before and after loading), the corresponding deformation can be evaluated. The application of ESPI in several laboratory tests on NBS is presented in this paper. In particular, during bending tests performed on geometrically similar NBS specimens, it was observed that the size and shape of the localized damage zone do not depend on the specimen size. These results allow for an interpretation of the 'size- effect,' which consists of a reduction of nominal strength as the specimen size increases.

Food quality inspection by speckle decorrelation properties of bacteria colonies

NASA Astrophysics Data System (ADS)

Bianco, V.; Mandracchia, B.; Nazzaro, F.; Marchesano, V.; Gennari, O.; Paturzo, M.; Grilli, S.; Ferraro, P.

2017-06-01

The development of tools for rapid food quality inspection is a highly pursued goal. These could be valuable devices to be used by food producers in factories or the customers themselves in specific installations at the marketplace. Here we show how speckle patterns in coherent imaging systems can be can be employed as indicators of the presence of bacteria colonies contaminating food or water. Speckle decorrelation is induced by the self-propelling movement of these organisms when they interact with coherent light. Hence, their presence can be detected using a simple setup in a condition in which the single element cannot be imaged, but the properties of the ensemble can be exploited. Thanks to the small magnification factor we set, our system can inspect a large Field-of-View (FoV). We show the possibility to discriminate between fresh and contaminated food, thus paving the way to the rapid food quality testing by consumers at the marketplace.

Localized analysis of paint-coat drying using dynamic speckle interferometry

NASA Astrophysics Data System (ADS)

Sierra-Sosa, Daniel; Tebaldi, Myrian; Grumel, Eduardo; Rabal, Hector; Elmaghraby, Adel

2018-07-01

The paint-coating is part of several industrial processes, including the automotive industry, architectural coatings, machinery and appliances. These paint-coatings must comply with high quality standards, for this reason evaluation techniques from paint-coatings are in constant development. One important factor from the paint-coating process is the drying, as it has influence on the quality of final results. In this work we present an assessment technique based on the optical dynamic speckle interferometry, this technique allows for the temporal activity evaluation of the paint-coating drying process, providing localized information from drying. This localized information is relevant in order to address the drying homogeneity, optimal drying, and quality control. The technique relies in the definition of a new temporal history of the speckle patterns to obtain the local activity; this information is then clustered to provide a convenient indicative of different drying process stages. The experimental results presented were validated using the gravimetric drying curves

Measurement of absolute regional lung air volumes from near-field x-ray speckles.

PubMed

Leong, Andrew F T; Paganin, David M; Hooper, Stuart B; Siew, Melissa L; Kitchen, Marcus J

2013-11-18

Propagation-based phase contrast x-ray (PBX) imaging yields high contrast images of the lung where airways that overlap in projection coherently scatter the x-rays, giving rise to a speckled intensity due to interference effects. Our previous works have shown that total and regional changes in lung air volumes can be accurately measured from two-dimensional (2D) absorption or phase contrast images when the subject is immersed in a water-filled container. In this paper we demonstrate how the phase contrast speckle patterns can be used to directly measure absolute regional lung air volumes from 2D PBX images without the need for a water-filled container. We justify this technique analytically and via simulation using the transport-of-intensity equation and calibrate the technique using our existing methods for measuring lung air volume. Finally, we show the full capabilities of this technique for measuring regional differences in lung aeration.

Demonstration of Numerical Equivalence of Ensemble and Spectral Averaging in Electromagnetic Scattering by Random Particulate Media

NASA Technical Reports Server (NTRS)

Mishchenko, Michael I.; Dlugach, Janna M.; Zakharova, Nadezhda T.

2016-01-01

The numerically exact superposition T-matrix method is used to model far-field electromagnetic scattering by two types of particulate object. Object 1 is a fixed configuration which consists of N identical spherical particles (with N 200 or 400) quasi-randomly populating a spherical volume V having a median size parameter of 50. Object 2 is a true discrete random medium (DRM) comprising the same number N of particles randomly moving throughout V. The median particle size parameter is fixed at 4. We show that if Object 1 is illuminated by a quasi-monochromatic parallel beam then it generates a typical speckle pattern having no resemblance to the scattering pattern generated by Object 2. However, if Object 1 is illuminated by a parallel polychromatic beam with a 10 bandwidth then it generates a scattering pattern that is largely devoid of speckles and closely reproduces the quasi-monochromatic pattern generated by Object 2. This result serves to illustrate the capacity of the concept of electromagnetic scattering by a DRM to encompass fixed quasi-random particulate samples provided that they are illuminated by polychromatic light.

Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging of biological cells

NASA Astrophysics Data System (ADS)

Di, Jianglei; Song, Yu; Xi, Teli; Zhang, Jiwei; Li, Ying; Ma, Chaojie; Wang, Kaiqiang; Zhao, Jianlin

2017-11-01

Biological cells are usually transparent with a small refractive index gradient. Digital holographic interferometry can be used in the measurement of biological cells. We propose a dual-wavelength common-path digital holographic microscopy for the quantitative phase imaging of biological cells. In the proposed configuration, a parallel glass plate is inserted in the light path to create the lateral shearing, and two lasers with different wavelengths are used as the light source to form the dual-wavelength composite digital hologram. The information of biological cells for different wavelengths is separated and extracted in the Fourier domain of the hologram, and then combined to a shorter wavelength in the measurement process. This method could improve the system's temporal stability and reduce speckle noises simultaneously. Mouse osteoblastic cells and peony pollens are measured to show the feasibility of this method.

Shaping nanoscale magnetic domain memory in exchange-coupled ferromagnets by field cooling.

PubMed

Chesnel, Karine; Safsten, Alex; Rytting, Matthew; Fullerton, Eric E

2016-06-01

The advance of magnetic nanotechnologies relies on detailed understanding of nanoscale magnetic mechanisms in materials. Magnetic domain memory (MDM), that is, the tendency for magnetic domains to repeat the same pattern during field cycling, is important for magnetic recording technologies. Here we demonstrate MDM in [Co/Pd]/IrMn films, using coherent X-ray scattering. Under illumination, the magnetic domains in [Co/Pd] produce a speckle pattern, a unique fingerprint of their nanoscale configuration. We measure MDM by cross-correlating speckle patterns throughout magnetization processes. When cooled below its blocking temperature, the film exhibits up to 100% MDM, induced by exchange-coupling with the underlying IrMn layer. The degree of MDM drastically depends on cooling conditions. If the film is cooled under moderate fields, MDM is high throughout the entire magnetization loop. If the film is cooled under nearly saturating field, MDM vanishes, except at nucleation and saturation. Our findings show how to fully control the occurrence of MDM by field cooling.

Shaping nanoscale magnetic domain memory in exchange-coupled ferromagnets by field cooling

DOE PAGES

Chesnel, Karine; Safsten, Alex; Rytting, Matthew; ...

2016-06-01

The advance of magnetic nanotechnologies relies on detailed understanding of nanoscale magnetic mechanisms in materials. Magnetic domain memory (MDM), that is, the tendency for magnetic domains to repeat the same pattern during field cycling, is important for magnetic recording technologies. Here we demonstrate MDM in [Co/Pd]/IrMn films, using coherent X-ray scattering. Under illumination, the magnetic domains in [Co/Pd] produce a speckle pattern, a unique fingerprint of their nanoscale configuration. We measure MDM by cross-correlating speckle patterns throughout magnetization processes. When cooled below its blocking temperature, the film exhibits up to 100% MDM, induced by exchange-coupling with the underlying IrMn layer.more » The degree of MDM drastically depends on cooling conditions. If the film is cooled under moderate fields, MDM is high throughout the entire magnetization loop. Lastly, if the film is cooled under nearly saturating field, MDM vanishes, except at nucleation and saturation. Our findings show how to fully control the occurrence of MDM by field cooling.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chesnel, Karine; Safsten, Alex; Rytting, Matthew

The advance of magnetic nanotechnologies relies on detailed understanding of nanoscale magnetic mechanisms in materials. Magnetic domain memory (MDM), that is, the tendency for magnetic domains to repeat the same pattern during field cycling, is important for magnetic recording technologies. Here we demonstrate MDM in [Co/Pd]/IrMn films, using coherent X-ray scattering. Under illumination, the magnetic domains in [Co/Pd] produce a speckle pattern, a unique fingerprint of their nanoscale configuration. We measure MDM by cross-correlating speckle patterns throughout magnetization processes. When cooled below its blocking temperature, the film exhibits up to 100% MDM, induced by exchange-coupling with the underlying IrMn layer.more » The degree of MDM drastically depends on cooling conditions. If the film is cooled under moderate fields, MDM is high throughout the entire magnetization loop. Lastly, if the film is cooled under nearly saturating field, MDM vanishes, except at nucleation and saturation. Our findings show how to fully control the occurrence of MDM by field cooling.« less

Shaping nanoscale magnetic domain memory in exchange-coupled ferromagnets by field cooling

PubMed Central

Chesnel, Karine; Safsten, Alex; Rytting, Matthew; Fullerton, Eric E.

2016-01-01

The advance of magnetic nanotechnologies relies on detailed understanding of nanoscale magnetic mechanisms in materials. Magnetic domain memory (MDM), that is, the tendency for magnetic domains to repeat the same pattern during field cycling, is important for magnetic recording technologies. Here we demonstrate MDM in [Co/Pd]/IrMn films, using coherent X-ray scattering. Under illumination, the magnetic domains in [Co/Pd] produce a speckle pattern, a unique fingerprint of their nanoscale configuration. We measure MDM by cross-correlating speckle patterns throughout magnetization processes. When cooled below its blocking temperature, the film exhibits up to 100% MDM, induced by exchange-coupling with the underlying IrMn layer. The degree of MDM drastically depends on cooling conditions. If the film is cooled under moderate fields, MDM is high throughout the entire magnetization loop. If the film is cooled under nearly saturating field, MDM vanishes, except at nucleation and saturation. Our findings show how to fully control the occurrence of MDM by field cooling. PMID:27248368

Integration of wings and their eyespots in the speckled wood butterfly Pararge aegeria.

PubMed

Breuker, Casper J; Gibbs, Melanie; Van Dyck, Hans; Brakefield, Paul M; Klingenberg, Christian Peter; Van Dongen, Stefan

2007-07-15

We investigated both the phenotypic and developmental integration of eyespots on the fore- and hindwings of speckled wood butterflies Pararge aegeria. Eyespots develop within a framework of wing veins, which may not only separate eyespots developmentally, but may at the same time also integrate them by virtue of being both signalling sources and barriers during eyespot development. We therefore specifically investigated the interaction between wing venation patterns and eyespot integration. Phenotypic covariation among eyespots was very high, but only eyespots in neighbouring wing cells and in homologous wing cells on different wing surfaces were developmentally integrated. This can be explained by the fact that the wing cells of these eyespots share one or more wing veins. The wing venation patterns of fore- and hindwings were highly integrated, both phenotypically and developmentally. This did not affect overall developmental integration of the eyespots. The adaptive significance of integration patterns is discussed and more specifically we stress the need to conduct studies on phenotypic plasticity of integration.

Stimulated Raman and Brillouin scattering of polarization-smoothed and temporally smoothed laser beams

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berger, R.L.; Lefebvre, E.; Langdon, A.B.

1999-04-01

Control of filamentation and stimulated Raman and Brillouin scattering is shown to be possible by use of both spatial and temporal smoothing schemes. The spatial smoothing is accomplished by the use of phase plates [Y. Kato and K. Mima, Appl. Phys. {bold 329}, 186 (1982)] and polarization smoothing [Lefebvre {ital et al.}, Phys. Plasmas {bold 5}, 2701 (1998)] in which the plasma is irradiated with two orthogonally polarized, uncorrelated speckle patterns. The temporal smoothing considered here is smoothing by spectral dispersion [Skupsky {ital et al.}, J. Appl. Phys. {bold 66}, 3456 (1989)] in which the speckle pattern changes on themore » laser coherence time scale. At the high instability gains relevant to laser fusion experiments, the effect of smoothing must include the competition among all three instabilities. {copyright} {ital 1999 American Institute of Physics.}« less

Experimental study on nonlinear vibrating of aluminum foam using electronic speckle pattern interferometry

NASA Astrophysics Data System (ADS)

Yang, Fujun; Ma, Yinhang; Tao, Nan; He, Xiaoyuan

2017-06-01

Due to its multi properties, including excellent stiffness-to-weight and strength-to-weight ratios, closed-cell aluminum and its alloy foams become candidate materials for use in many high-technology industries, such as the automotive and aerospace industries. For the efficient use of closed-cell foams in structural applications, it is necessary and important to detailly understand their mechanical characteristics. In this paper, the nonlinear vibration responses of the cantilever beams of closed-cell aluminum foams were investigated by use of electronic speckle pattern interferometry (ESPI). The nonlinear resonant mode shapes of testing specimens under harmonic excitation were measured. It is first time to obtain from the experimental results that there exist super-harmonic responses when the cantilever beams of closed-cell aluminum foam were forced to vibrate, which was caused by its specific cellular structures.

Optical image hiding based on computational ghost imaging

NASA Astrophysics Data System (ADS)

Wang, Le; Zhao, Shengmei; Cheng, Weiwen; Gong, Longyan; Chen, Hanwu

2016-05-01

Imaging hiding schemes play important roles in now big data times. They provide copyright protections of digital images. In the paper, we propose a novel image hiding scheme based on computational ghost imaging to have strong robustness and high security. The watermark is encrypted with the configuration of a computational ghost imaging system, and the random speckle patterns compose a secret key. Least significant bit algorithm is adopted to embed the watermark and both the second-order correlation algorithm and the compressed sensing (CS) algorithm are used to extract the watermark. The experimental and simulation results show that the authorized users can get the watermark with the secret key. The watermark image could not be retrieved when the eavesdropping ratio is less than 45% with the second-order correlation algorithm, whereas it is less than 20% with the TVAL3 CS reconstructed algorithm. In addition, the proposed scheme is robust against the 'salt and pepper' noise and image cropping degradations.

Image Science Research for Speckle-based LADAR (Speckle Research for 3D Imaging LADAR)

DTIC Science & Technology

2008-04-03

INVARIANT + FERGUS, TORRALBA, AND FREEMAN. MIT-CSAIL-TR-2006-058 MAP DETECTOR PATTERN FOR EACH POINT IN OBJECT SPACE DEBLURRING PROBLEM IMPULSE RESPONSE...GENERALIZED THEORY FOR THE LOGARITHMIC ASPHERE ( )( ) it e φ ρρ −= IMPULSE RESPONSE (PSF) 2 2 2 2 0 0 22 2( ) 2 2 0 2 2 2 2 2 2 0 0 2 ( ; ) 2 ( ) i s i i t R...ascent; γ=1, Burch, Skilling, Gull; Loops needed Noise deviation Area of PSF New parameter L σ A γ COMPARISON OF MAXIMUM ENTROPY METHODS † † W. CHI

Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture

PubMed Central

Sun, Hao; Merrill, Daniel; An, Ran; Turek, John; Matei, Daniela; Nolte, David D.

2017-01-01

Abstract. Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a high-content 3-D optical imaging technology based on low-coherence interferometry and digital holography that uses dynamic speckle as high-content image contrast to probe deep inside 3-D tissue. Speckle contrast is shown to be a scaling function of the acquisition time relative to the persistence time of intracellular transport and hence provides a measure of cellular activity. Cellular responses of 3-D multicellular spheroids to paclitaxel are compared among three different growth techniques: rotating bioreactor (BR), hanging-drop (HD), and nonadherent (U-bottom, UB) plate spheroids, compared with ex vivo living tissues. HD spheroids have the most homogeneous tissue, whereas BR spheroids display large sample-to-sample variability as well as spatial heterogeneity. The responses of BR-grown tumor spheroids to paclitaxel are more similar to those of ex vivo biopsies than the responses of spheroids grown using HD or plate methods. The rate of mitosis inhibition by application of taxol is measured through tissue dynamics spectroscopic imaging, demonstrating the ability to monitor antimitotic chemotherapy. These results illustrate the potential use of low-coherence digital holography for 3-D pharmaceutical screening applications. PMID:28301634

Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture

NASA Astrophysics Data System (ADS)

Sun, Hao; Merrill, Daniel; An, Ran; Turek, John; Matei, Daniela; Nolte, David D.

2017-01-01

Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a high-content 3-D optical imaging technology based on low-coherence interferometry and digital holography that uses dynamic speckle as high-content image contrast to probe deep inside 3-D tissue. Speckle contrast is shown to be a scaling function of the acquisition time relative to the persistence time of intracellular transport and hence provides a measure of cellular activity. Cellular responses of 3-D multicellular spheroids to paclitaxel are compared among three different growth techniques: rotating bioreactor (BR), hanging-drop (HD), and nonadherent (U-bottom, UB) plate spheroids, compared with ex vivo living tissues. HD spheroids have the most homogeneous tissue, whereas BR spheroids display large sample-to-sample variability as well as spatial heterogeneity. The responses of BR-grown tumor spheroids to paclitaxel are more similar to those of ex vivo biopsies than the responses of spheroids grown using HD or plate methods. The rate of mitosis inhibition by application of taxol is measured through tissue dynamics spectroscopic imaging, demonstrating the ability to monitor antimitotic chemotherapy. These results illustrate the potential use of low-coherence digital holography for 3-D pharmaceutical screening applications.

Three-Dimensional Dynamic Deformation Measurements Using Stereoscopic Imaging and Digital Speckle Photography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prentice, H. J.; Proud, W. G.

2006-07-28

A technique has been developed to determine experimentally the three-dimensional displacement field on the rear surface of a dynamically deforming plate. The technique combines speckle analysis with stereoscopy, using a modified angular-lens method: this incorporates split-frame photography and a simple method by which the effective lens separation can be adjusted and calibrated in situ. Whilst several analytical models exist to predict deformation in extended or semi-infinite targets, the non-trivial nature of the wave interactions complicates the generation and development of analytical models for targets of finite depth. By interrogating specimens experimentally to acquire three-dimensional strain data points, both analytical andmore » numerical model predictions can be verified more rigorously. The technique is applied to the quasi-static deformation of a rubber sheet and dynamically to Mild Steel sheets of various thicknesses.« less

The role of photographic parameters in laser speckle or particle image displacement velocimetry

NASA Technical Reports Server (NTRS)

Lourenco, L.; Krothapalli, A.

1987-01-01

The parameters involved in obtaining the multiple exposure photographs in the laser speckle velocimetry method (to record the light scattering by the seeding particles) were optimized. The effects of the type, concentration, and dimensions of the tracer, the exposure conditions (time between exposures, exposure time, and number of exposures), and the sensitivity and resolution of the film on the quality of the final results were investigated, photographing an experimental flow behind an impulsively started circular cylinder. The velocity data were acquired by digital processing of Young's fringes, produced by point-by-point scanning of a photographic negative. Using the optimal photographing conditions, the errors involved in the estimation of the fringe angle and spacing were of the order of 1 percent for the spacing and +/1 deg for the fringe orientation. The resulting accuracy in the velocity was of the order of 2-3 percent of the maximum velocity in the field.

Shunt flow evaluation in congenital heart disease based on two-dimensional speckle tracking.

PubMed

Fadnes, Solveig; Nyrnes, Siri Ann; Torp, Hans; Lovstakken, Lasse

2014-10-01

High-frame-rate ultrasound speckle tracking was used for quantification of peak velocity in shunt flows resulting from septal defects in congenital heart disease. In a duplex acquisition scheme implemented on a research scanner, unfocused transmit beams and full parallel receive beamforming were used to achieve a frame rate of 107 frames/s for full field-of-view flow images with high accuracy, while also ensuring high-quality focused B-mode tissue imaging. The setup was evaluated in vivo for neonates with atrial and ventricular septal defects. The shunt position was automatically tracked in B-mode images and further used in blood speckle tracking to obtain calibrated shunt flow velocities throughout the cardiac cycle. Validation toward color flow imaging and pulsed wave Doppler with manual angle correction indicated that blood speckle tracking could provide accurate estimates of shunt flow velocities. The approach was less biased by clutter filtering compared with color flow imaging and was able to provide velocity estimates beyond the Nyquist range. Possible placements of sample volumes (and angle corrections) for conventional Doppler resulted in a peak shunt velocity variations of 0.49-0.56 m/s for the ventricular septal defect of patient 1 and 0.38-0.58 m/s for the atrial septal defect of patient 2. In comparison, the peak velocities found from speckle tracking were 0.77 and 0.33 m/s for patients 1 and 2, respectively. Results indicated that complex intraventricular flow velocity patterns could be quantified using high-frame-rate speckle tracking of both blood and tissue movement. This could potentially help increase diagnostic accuracy and decrease inter-observer variability when measuring peak velocity in shunt flows. Copyright © 2014 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Correcting speckle contrast at small speckle size to enhance signal to noise ratio for laser speckle contrast imaging.

PubMed

Qiu, Jianjun; Li, Yangyang; Huang, Qin; Wang, Yang; Li, Pengcheng

2013-11-18

In laser speckle contrast imaging, it was usually suggested that speckle size should exceed two camera pixels to eliminate the spatial averaging effect. In this work, we show the benefit of enhancing signal to noise ratio by correcting the speckle contrast at small speckle size. Through simulations and experiments, we demonstrated that local speckle contrast, even at speckle size much smaller than one pixel size, can be corrected through dividing the original speckle contrast by the static speckle contrast. Moreover, we show a 50% higher signal to noise ratio of the speckle contrast image at speckle size below 0.5 pixel size than that at speckle size of two pixels. These results indicate the possibility of selecting a relatively large aperture to simultaneously ensure sufficient light intensity and high accuracy and signal to noise ratio, making the laser speckle contrast imaging more flexible.

Time-resolved x-ray imaging of a laser-induced nanoplasma and its neutral residuals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fluckiger, L.; Rupp, D.; Adolph, M.

The evolution of individual, large gas-phase xenon clusters, turned into a nanoplasma by a high power infrared laser pulse, is tracked from femtoseconds up to nanoseconds after laser excitation via coherent diffractive imaging, using ultra-short soft x-ray free electron laser pulses. A decline of scattering signal at high detection angles with increasing time delay indicates a softening of the cluster surface. Here we demonstrate, for the first time a representative speckle pattern of a new stage of cluster expansion for xenon clusters after a nanosecond irradiation. The analysis of the measured average speckle size and the envelope of the intensitymore » distribution reveals a mean cluster size and length scale of internal density fluctuations. Furthermore, the measured diffraction patterns were reproduced by scattering simulations which assumed that the cluster expands with pronounced internal density fluctuations hundreds of picoseconds after excitation.« less

Analysis of mechanical behavior of implant-supported prostheses in the anterior maxilla: analysis by speckle pattern interferometry

NASA Astrophysics Data System (ADS)

Corrêa, Cássia B.; Ramos, Nuno V.; Monteiro, Jaime; Vaz, Luis G.; Vaz, Mario A. P.

2012-10-01

The use of implants to rehabilitation of total edentulous, partial edentulous or single tooth is increasing, it is due to the high rate of success that this type of treatment present. The objective of this study was to analyze the mechanical behavior of different positions of two dental implants in a rehabilitation of 4 teeth in the region of maxilla anterior. The groups studied were divided according the positioning of the implants. The Group 1: Internal Hexagonal implant in position of lateral incisors and pontic in region of central incisors; Group 2: Internal Hexagonal implant in position of central incisors and cantilever of the lateral incisors and Group3 - : Internal Hexagonal implants alternate with suspended elements. The Electronic Speckle Pattern Interferometry (ESPI) technique was selected for the mechanical evaluation of the 3 groups performance. The results are shown in interferometric phase maps representing the displacement field of the prosthetic structure.

Time-resolved x-ray imaging of a laser-induced nanoplasma and its neutral residuals

DOE PAGES

Fluckiger, L.; Rupp, D.; Adolph, M.; ...

2016-04-13

The evolution of individual, large gas-phase xenon clusters, turned into a nanoplasma by a high power infrared laser pulse, is tracked from femtoseconds up to nanoseconds after laser excitation via coherent diffractive imaging, using ultra-short soft x-ray free electron laser pulses. A decline of scattering signal at high detection angles with increasing time delay indicates a softening of the cluster surface. Here we demonstrate, for the first time a representative speckle pattern of a new stage of cluster expansion for xenon clusters after a nanosecond irradiation. The analysis of the measured average speckle size and the envelope of the intensitymore » distribution reveals a mean cluster size and length scale of internal density fluctuations. Furthermore, the measured diffraction patterns were reproduced by scattering simulations which assumed that the cluster expands with pronounced internal density fluctuations hundreds of picoseconds after excitation.« less

The use of holographic interferometry and electron speckle pattern interferometry for diffusion measurement in biochemical and pharmaceutical engineering applications

NASA Astrophysics Data System (ADS)

Axelsson, Anders; Marucci, Mariagrazia

2008-12-01

In this review holographic interferometry and electron speckle pattern interferometry are discussed as efficient techniques for diffusion measurements in biochemical and pharmaceutical applications. Transport phenomena can be studied, quantitatively and qualitatively, in gels, liquids and membranes. Detailed information on these phenomena is required to design effective chromatography bioseparation processes using gel beads or ultrafiltration membranes, and in the design of controlled-release pharmaceuticals using membrane-coated pellets or tablets. The influence of gel concentration, ion strength in the liquid and the size of diffusing protein molecules can easily be studied with good accuracy. When studying membranes, the resistance can be quantified, and it is also possible to discriminate between permeable and semi-permeable membranes. In this review the influence of temperature, natural convection and light deflection on the accuracy of the diffusion measurements is also discussed.

Experimental study on deformation field evolution in rock sample with en echelon faults using digital speckle correlation method

NASA Astrophysics Data System (ADS)

Ma, S.; Ma, J.; Liu, L.; Liu, P.

2007-12-01

Digital speckle correlation method (DSCM) is one kind of photomechanical deformation measurement method. DSCM could obtain continuous deformation field contactlessly by just capturing speckle images from specimen surface. Therefore, it is suitable to observe high spatial resolution deformation field in tectonophysical experiment. However, in the general DSCM experiment, the inspected surface of specimen needs to be painted to bear speckle grains in order to obtain the high quality speckle image. This also affects the realization of other measurement techniques. In this study, an improved DSCM system is developed and utilized to measure deformation field of rock specimen without surface painting. The granodiorite with high contrast nature grains is chosen to manufacture the specimen, and a specially designed DSCM algorithm is developed to analyze this kind of nature speckle images. Verification and calibration experiments show that the system could inspect a continuous (about 15Hz) high resolution displacement field (with resolution of 5μm) and strain field (with resolution of 50μɛ), dispensing with any preparation on rock specimen. Therefore, it could be conveniently utilized to study the failure of rock structure. Samples with compressive en echelon faults and extensional en echelon faults are studied on a two-direction servo-control test machine. The failure process of the samples is discussed based on the DSCM results. Experiment results show that: 1) The contours of displacement field could clearly indicate the activities of faults and new cracks. The displacement gradient adjacent to active faults and cracks is much greater than other areas. 2) Before failure of the samples, the mean strain of the jog area is largest for the compressive en echelon fault, while that is smallest for the extensional en echelon fault. This consists with the understanding that the jog area of compressive fault subjects to compression and that of extensional fault subjects to tension. 3) For the extensional en echelon sample, the dislocation across fault on load-driving end is greater than that cross fault on fixed end. Within the same fault, the dislocation across branch far from the jog area is greater than that across branch near the jog area. This indicates the restriction effect of jog area on the activity of fault. Moreover, the average dislocation across faults is much greater than that across the cracks. 4) For the compressive en echelon fault, the wing cracks initialized firstly and propagate outwards the jog area. Subsequently, a wedge strain concentration area is initialized and developed in the jog area because of the interaction of the two faults. Finally, the jog area failed when one crack propagates rapidly and connects the two ends of faults. The DSCM system used in this study could clearly show the deformation and failure process of the en echelon fault sample. The experiment using DSCM could be performed dispensing with any preparation on specimen and not affecting other inspection. Therefore, DSCM is expected to be a suitable tool for experimental study of fault samples in laboratory.

Automatic focusing in digital holography and its application to stretched holograms.

PubMed

Memmolo, P; Distante, C; Paturzo, M; Finizio, A; Ferraro, P; Javidi, B

2011-05-15

The searching and recovering of the correct reconstruction distance in digital holography (DH) can be a cumbersome and subjective procedure. Here we report on an algorithm for automatically estimating the in-focus image and recovering the correct reconstruction distance for speckle holograms. We have tested the approach in determining the reconstruction distances of stretched digital holograms. Stretching a hologram with a variable elongation parameter makes it possible to change the in-focus distance of the reconstructed image. In this way, the proposed algorithm can be verified at different distances by dispensing the recording of different holograms. Experimental results are shown with the aim of demonstrating the usefulness of the proposed method, and a comparative analysis has been performed with respect to other existing algorithms developed for DH. © 2011 Optical Society of America

Processing techniques for digital sonar images from GLORIA.

USGS Publications Warehouse

Chavez, P.S.

1986-01-01

Image processing techniques have been developed to handle data from one of the newest members of the remote sensing family of digital imaging systems. This paper discusses software to process data collected by the GLORIA (Geological Long Range Inclined Asdic) sonar imaging system, designed and built by the Institute of Oceanographic Sciences (IOS) in England, to correct for both geometric and radiometric distortions that exist in the original 'raw' data. Preprocessing algorithms that are GLORIA-specific include corrections for slant-range geometry, water column offset, aspect ratio distortion, changes in the ship's velocity, speckle noise, and shading problems caused by the power drop-off which occurs as a function of range.-from Author

Body Size Evolution in Insular Speckled Rattlesnakes (Viperidae: Crotalus mitchellii)

PubMed Central

Meik, Jesse M.; Lawing, A. Michelle; Pires-daSilva, André

2010-01-01

Background Speckled rattlesnakes (Crotalus mitchellii) inhabit multiple islands off the coast of Baja California, Mexico. Two of the 14 known insular populations have been recognized as subspecies based primarily on body size divergence from putative mainland ancestral populations; however, a survey of body size variation from other islands occupied by these snakes has not been previously reported. We examined body size variation between island and mainland speckled rattlesnakes, and the relationship between body size and various island physical variables among 12 island populations. We also examined relative head size among giant, dwarfed, and mainland speckled rattlesnakes to determine whether allometric differences conformed to predictions of gape size (and indirectly body size) evolving in response to shifts in prey size. Methodology/Principal Findings Insular speckled rattlesnakes show considerable variation in body size when compared to mainland source subspecies. In addition to previously known instances of gigantism on Ángel de la Guarda and dwarfism on El Muerto, various degrees of body size decrease have occurred frequently in this taxon, with dwarfed rattlesnakes occurring mostly on small, recently isolated, land-bridge islands. Regression models using the Akaike information criterion (AIC) showed that mean SVL of insular populations was most strongly correlated with island area, suggesting the influence of selection for different body size optima for islands of different size. Allometric differences in head size of giant and dwarf rattlesnakes revealed patterns consistent with shifts to larger and smaller prey, respectively. Conclusions/Significance Our data provide the first example of a clear relationship between body size and island area in a squamate reptile species; among vertebrates this pattern has been previously documented in few insular mammals. This finding suggests that selection for body size is influenced by changes in community dynamics that are related to graded differences in area over what are otherwise similar bioclimatic conditions. We hypothesize that in this system shifts to larger prey, episodic saturation and depression of primary prey density, and predator release may have led to insular gigantism, and that shifts to smaller prey and increased reproductive efficiency in the presence of intense intraspecific competition may have led to insular dwarfism. PMID:20209105

Modal noise in multimode optical fibers

NASA Astrophysics Data System (ADS)

Rawson, E. G.; Goodman, J. W.

1983-03-01

A changing speckle pattern exists at the output of a multimode optical fiber if the optical source is sufficiently coherent. When spatial filtration (for example, at a misaligned connector) or polarization filtration (for example, in certain access couplers) occurs in the presence of such speckle, the optical signal power fluctuates; such fluctuations are called 'modal noise'. This paper reviews modal noise theory and experiment, including the prediction and measurement of the modal noise signal-to-noise ratio in the presence of spatial filtration and constrained total guided power. It also presents new results relating to modal noise effects in fiber branching devices such as star couplers, access couplers, and power dividers.

Efficient image projection by Fourier electroholography.

PubMed

Makowski, Michał; Ducin, Izabela; Kakarenko, Karol; Kolodziejczyk, Andrzej; Siemion, Agnieszka; Siemion, Andrzej; Suszek, Jaroslaw; Sypek, Maciej; Wojnowski, Dariusz

2011-08-15

An improved efficient projection of color images is presented. It uses a phase spatial light modulator with three iteratively optimized Fourier holograms displayed simultaneously--each for one primary color. This spatial division instead of time division provides stable images. A pixelated structure of the modulator and fluctuations of liquid crystal molecules cause a zeroth-order peak, eliminated by additional wavelength-dependent phase factors shifting it before the image plane, where it is blocked with a matched filter. Speckles are suppressed by time integration of variable speckle patterns generated by additional randomizations of an initial phase and minor changes of the signal. © 2011 Optical Society of America

Photorefractive detection of tagged photons in ultrasound modulated optical tomography of thick biological tissues.

PubMed

Ramaz, F; Forget, B; Atlan, M; Boccara, A C; Gross, M; Delaye, P; Roosen, G

2004-11-01

We present a new and simple method to obtain ultrasound modulated optical tomography images in thick biological tissues with the use of a photorefractive crystal. The technique offers the advantage of spatially adapting the output speckle wavefront by analysing the signal diffracted by the interference pattern between this output field and a reference beam, recorded inside the photorefractive crystal. Averaging out due to random phases of the speckle grains vanishes, and we can use a fast single photodetector to measure the ultrasound modulated optical contrast. This technique offers a promising way to make direct measurements within the decorrelation time scale of living tissues.

“Beating speckles” via electrically-induced vibrations of Au nanorods embedded in sol-gel

PubMed Central

Ritenberg, Margarita; Beilis, Edith; Ilovitsh, Asaf; Barkai, Zehava; Shahmoon, Asaf; Richter, Shachar; Zalevsky, Zeev; Jelinek, Raz

2014-01-01

Generation of macroscopic phenomena through manipulating nano-scale properties of materials is among the most fundamental goals of nanotechnology research. We demonstrate cooperative “speckle beats” induced through electric-field modulation of gold (Au) nanorods embedded in a transparent sol-gel host. Specifically, we show that placing the Au nanorod/sol-gel matrix in an alternating current (AC) field gives rise to dramatic modulation of incident light scattered from the material. The speckle light patterns take form of “beats”, for which the amplitude and frequency are directly correlated with the voltage and frequency, respectively, of the applied AC field. The data indicate that the speckle beats arise from localized vibrations of the gel-embedded Au nanorods, induced through the interactions between the AC field and the electrostatically-charged nanorods. This phenomenon opens the way for new means of investigating nanoparticles in constrained environments. Applications in electro-optical devices, such as optical modulators, movable lenses, and others are also envisaged. PMID:24413086

Single shot speckle and coherence analysis of the hard X-ray free electron laser LCLS

DOE PAGES

Lee, Sooheyong; Roseker, W.; Gutt, C.; ...

2013-10-08

The single shot based coherence properties of hard x-ray pulses from the Linac Coherent Light Source (LCLS) were measured by analyzing coherent diffraction patterns from nano-particles and gold nanopowder. The intensity histogram of the small angle x-ray scattering ring from nano-particles reveals the fully transversely coherent nature of the LCLS beam with a number of transverse modemore » $$\\langle$$M s$$\\rangle$$ = 1.1. On the other hand, the speckle contrasts measured at a large wavevector yields information about the longitudinal coherence of the LCLS radiation after a silicon (111) monochromator. The quantitative agreement between our data and the simulation confirms a mean coherence time of 2.2 fs and a x-ray pulse duration of 29 fs. Lastly the observed reduction of the speckle contrast generated by x-rays with pulse duration longer than 30 fs indicates ultrafast dynamics taking place at an atomic length scale prior to the permanent sample damage.« less

Exploiting the speckle-correlation scattering matrix for a compact reference-free holographic image sensor

PubMed Central

Lee, KyeoReh; Park, YongKeun

2016-01-01

The word ‘holography' means a drawing that contains all of the information for light—both amplitude and wavefront. However, because of the insufficient bandwidth of current electronics, the direct measurement of the wavefront of light has not yet been achieved. Though reference-field-assisted interferometric methods have been utilized in numerous applications, introducing a reference field raises several fundamental and practical issues. Here we demonstrate a reference-free holographic image sensor. To achieve this, we propose a speckle-correlation scattering matrix approach; light-field information passing through a thin disordered layer is recorded and retrieved from a single-shot recording of speckle intensity patterns. Self-interference via diffusive scattering enables access to impinging light-field information, when light transport in the diffusive layer is precisely calibrated. As a proof-of-concept, we demonstrate direct holographic measurements of three-dimensional optical fields using a compact device consisting of a regular image sensor and a diffusor. PMID:27796290

Exploiting the speckle-correlation scattering matrix for a compact reference-free holographic image sensor.

PubMed

Lee, KyeoReh; Park, YongKeun

2016-10-31

The word 'holography' means a drawing that contains all of the information for light-both amplitude and wavefront. However, because of the insufficient bandwidth of current electronics, the direct measurement of the wavefront of light has not yet been achieved. Though reference-field-assisted interferometric methods have been utilized in numerous applications, introducing a reference field raises several fundamental and practical issues. Here we demonstrate a reference-free holographic image sensor. To achieve this, we propose a speckle-correlation scattering matrix approach; light-field information passing through a thin disordered layer is recorded and retrieved from a single-shot recording of speckle intensity patterns. Self-interference via diffusive scattering enables access to impinging light-field information, when light transport in the diffusive layer is precisely calibrated. As a proof-of-concept, we demonstrate direct holographic measurements of three-dimensional optical fields using a compact device consisting of a regular image sensor and a diffusor.

Laser speckle spatiotemporal variance analysis for noninvasive widefield measurements of blood pulsation and pulse rate on a camera-phone.

PubMed

Remer, Itay; Bilenca, Alberto

2015-11-01

Photoplethysmography is a well-established technique for the noninvasive measurement of blood pulsation. However, photoplethysmographic devices typically need to be in contact with the surface of the tissue and provide data from a single contact point. Extensions of conventional photoplethysmography to measurements over a wide field-of-view exist, but require advanced signal processing due to the low signal-to-noise-ratio of the photoplethysmograms. Here, we present a noncontact method based on temporal sampling of time-integrated speckle using a camera-phone for noninvasive, widefield measurements of physiological parameters across the human fingertip including blood pulsation and resting heart-rate frequency. The results show that precise estimation of these parameters with high spatial resolution is enabled by measuring the local temporal variation of speckle patterns of backscattered light from subcutaneous skin, thereby opening up the possibility for accurate high resolution blood pulsation imaging on a camera-phone. Camera-phone laser speckle imager along with measured relative blood perfusion maps of a fingertip showing skin perfusion response to a pulse pressure applied to the upper arm. The figure is for illustration only; the imager was stabilized on a stand throughout the experiments. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Real-time acquisition and display of flow contrast using speckle variance optical coherence tomography in a graphics processing unit.

PubMed

Xu, Jing; Wong, Kevin; Jian, Yifan; Sarunic, Marinko V

2014-02-01

In this report, we describe a graphics processing unit (GPU)-accelerated processing platform for real-time acquisition and display of flow contrast images with Fourier domain optical coherence tomography (FDOCT) in mouse and human eyes in vivo. Motion contrast from blood flow is processed using the speckle variance OCT (svOCT) technique, which relies on the acquisition of multiple B-scan frames at the same location and tracking the change of the speckle pattern. Real-time mouse and human retinal imaging using two different custom-built OCT systems with processing and display performed on GPU are presented with an in-depth analysis of performance metrics. The display output included structural OCT data, en face projections of the intensity data, and the svOCT en face projections of retinal microvasculature; these results compare projections with and without speckle variance in the different retinal layers to reveal significant contrast improvements. As a demonstration, videos of real-time svOCT for in vivo human and mouse retinal imaging are included in our results. The capability of performing real-time svOCT imaging of the retinal vasculature may be a useful tool in a clinical environment for monitoring disease-related pathological changes in the microcirculation such as diabetic retinopathy.

Change detection for synthetic aperture radar images based on pattern and intensity distinctiveness analysis

NASA Astrophysics Data System (ADS)

Wang, Xiao; Gao, Feng; Dong, Junyu; Qi, Qiang

2018-04-01

Synthetic aperture radar (SAR) image is independent on atmospheric conditions, and it is the ideal image source for change detection. Existing methods directly analysis all the regions in the speckle noise contaminated difference image. The performance of these methods is easily affected by small noisy regions. In this paper, we proposed a novel change detection framework for saliency-guided change detection based on pattern and intensity distinctiveness analysis. The saliency analysis step can remove small noisy regions, and therefore makes the proposed method more robust to the speckle noise. In the proposed method, the log-ratio operator is first utilized to obtain a difference image (DI). Then, the saliency detection method based on pattern and intensity distinctiveness analysis is utilized to obtain the changed region candidates. Finally, principal component analysis and k-means clustering are employed to analysis pixels in the changed region candidates. Thus, the final change map can be obtained by classifying these pixels into changed or unchanged class. The experiment results on two real SAR images datasets have demonstrated the effectiveness of the proposed method.

Phase and amplitude modification of a laser beam by two deformable mirrors using conventional 4f image encryption techniques

NASA Astrophysics Data System (ADS)

Wu, Chensheng; Ko, Jonathan; Rzasa, John Robertson; Davis, Christopher C.

2017-08-01

The image encryption and decryption technique using lens components and random phase screens has attracted a great deal of research interest in the past few years. In general, the optical encryption technique can translate a positive image into an image with nearly a white speckle pattern that is impossible to decrypt. However, with the right keys as conjugated random phase screens, the white noise speckle pattern can be decoded into the original image. We find that the fundamental ideas in image encryption can be borrowed and applied to carry out beam corrections through turbulent channels. Based on our detailed analysis, we show that by using two deformable mirrors arranged in similar fashions as in the image encryption technique, a large number of controllable phase and amplitude distribution patterns can be generated from a collimated Gaussian beam. Such a result can be further coupled with wavefront sensing techniques to achieve laser beam correction against turbulence distortions. In application, our approach leads to a new type of phase conjugation mirror that could be beneficial for directed energy systems.

Phase-coherent elastic scattering of electromagnetic waves from a random array of resonant dielectric ridges on a dielectric substrate: Weak roughness limit

NASA Astrophysics Data System (ADS)

Danila, B.; McGurn, A. R.

2005-03-01

A theoretical discussion is given of the diffuse scattering of p -polarized electromagnetic waves from a vacuum-dielectric interface characterized by a one-dimensional disorder in the form of parallel, Gaussian shaped, dielectric ridges positioned at random on a planar semi-infinite dielectric substrate. The parameters of the surface roughness are chosen so that the surface is characterized as weakly rough with a low ridge concentration. The emphasis is on phase coherent features in the speckle pattern of light scattered from the surface. These features are determined from the intensity-intensity correlation function of the speckle pattern and are studied as functions of the frequency of light for frequencies near the dielectric frequency resonances of the ridge material. In the first part of the study, the ridges on the substrate are taken to be identical, made from either GaAs, NaF, or ZnS. The substrate for all cases is CdS. In a second set of studies, the heights and widths of the ridges are statistically distributed. The effects of these different types of randomness on the scattering from the random array of dielectric ridges is determined near the dielectric resonance frequency of the ridge material. The work presented is an extension of studies [A. B. McGurn and R. M. Fitzgerald, Phys. Rev. B 65, 155414 (2002)] that originally treated only the differential reflection coefficient of the diffuse scattering of light (not speckle correlation functions) from a system of identical ridges. The object of the present work is to demonstrate the effects of the dielectric frequency resonances of the ridge materials on the phase coherent features found in the speckle patterns of the diffusely scattered light. The dielectric frequency resonances are shown to enhance the observation of the weak localization of electromagnetic surface waves at the random interface. The frequencies treated in this work are in the infrared. Previous weak localization studies have concentrated mainly on the visible and ultraviolet.

Stimulated backward Raman scattering driven collectively by two picosecond laser pulses in a bi- or multi-speckle configuration

NASA Astrophysics Data System (ADS)

Glize, K.; Rousseaux, C.; Bénisti, D.; Dervieux, V.; Gremillet, L.; Baton, S. D.; Lancia, L.

2017-03-01

In this paper, we investigate, both experimentally and numerically, the backward stimulated Raman scattering (SRS) excited collectively by two laser pulses. The experiments have been carried out at the LULI facility using two co-propagating 1- μ m wavelength, 1.5- ps duration laser pulses focused in a preformed underdense plasma. A particular emphasis is laid on the configuration where the pulses are focused side-by-side, with a lateral distance of 80 - 90 μ m , but not simultaneously. It is experimentally demonstrated that a weak-intensity speckle, ineffective when fired alone in a preformed plasma, yields a significant SRS-induced reflectivity if launched a few picoseconds after a strong one. The data have been obtained by using both highly space-time resolved Thomson diagnostics and space-resolved SRS reflectivity measurements. By choosing either parallel or orthogonal polarizations for the two laser pulses, our experiments shed light on the role of either electrostatic or electromagnetic seeding in enhancing SRS from weak-intensity speckles. A major finding is that seeding operates over unexpectedly long times ( 15 - 20 ps under our experimental conditions). Similar results are obtained in lower-density plasmas, or when the weak pulse is smoothed by a random phase plate, thus leading to multiple speckle interaction, while the strong pulse is focused within the speckle pattern. The data are discussed with the help of particle-in-cell numerical simulations, which confirm the destabilizing effect of the strong pulse over the weak one after a short transient time.

Determining the mechanical properties of rat skin with digital image speckle correlation.

PubMed

Guan, E; Smilow, Sarah; Rafailovich, Miriam; Sokolov, Jonathan

2004-01-01

Accurate measurement of the mechanical properties of skin has numerous implications in surgical repair, dermal disorders and the diagnosis and treatment of trauma to the skin. Investigation of facial wrinkle formation, as well as research in the areas of skin aging and cosmetic product assessment can also benefit from alternative methodologies for the measurement of mechanical properties. A noncontact, noninvasive technique, digital image speckle correlation (DISC), has been successfully introduced to measure the deformation field of a skin sample loaded by a material test machine. With the force information obtained from the loading device, the mechanical properties of the skin, such as Young's modulus, linear limitation and material strength, can be calculated using elastic or viscoelastic theory. The DISC method was used to measure the deformation of neonatal rat skin, with and without a glycerin-fruit-oil-based cream under uniaxial tension. Deformation to failure procedure of newborn rat skin was recorded and analyzed. Single skin layer failures were observed and located by finding the strain concentration. Young's moduli of freshly excised rat skin, cream-processed rat skin and unprocessed rat skin, 24 h after excision, were found with tensile tests to be 1.6, 1.4 and 0.7 MPa, respectively. Our results have shown that DISC provides a novel technique for numerous applications in dermatology and reconstructive surgeries. Copyright 2004 S. Karger AG, Basel

Demodulator electronics for laser vibrometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dudzik, G.; Waz, A. T.; Kaczmarek, P. R.

2012-06-13

One of the most important parts of a fiber-laser vibrometer is demodulation electronic section. The distortion, nonlinearity, offset and added noise of measured signal come from electronic circuits and they have direct influence on finale measuring results. Two main parameters of an investigated vibrating object: velocity V(t) and displacement s(t), influence of detected beat signals. They are: the Doppler frequency deviation f(t) and phase shift {phi}(t), respectively. Because of wide range of deviations it is difficult to use just one demodulator. That is the reason why we use three different types of demodulators. The first one is the IQ demodulator,more » which is the most sensitive one and its output is proportional to the displacement. Each IQ channel is sampled simultaneously by an analog to digital converter (ADC) integrated in a digital signal processor (DSP). The output signals from the two FM demodulators are proportional to the frequency deviation of heterodyne signals. They are sensitive directly to the velocity of the object. The main disadvantage of scattered light interferometry system is a ''speckle effect'', appearing in relatively large amplitude fluctuation of a heterodyne signal. To minimize ''speckle effect'' influence on quality of beat signals we applied the automatic gain control (AGC) system. Data acquisition, further signal processing (e.g. vibration frequency spectra) and presentation of results is realized by PC via USB interface.« less

Effects of the source, surface, and sensor couplings and colorimetric of laser speckle pattern on the performance of optical imaging system

NASA Astrophysics Data System (ADS)

Darwiesh, M.; El-Sherif, Ashraf F.; El-Ghandour, Hatem; Aly, Hussein A.; Mokhtar, A. M.

2011-03-01

Optical imaging systems are widely used in different applications include tracking for portable scanners; input pointing devices for laptop computers, cell phones, and cameras, fingerprint-identification scanners, optical navigation for target tracking, and in optical computer mouse. We presented an experimental work to measure and analyze the laser speckle pattern (LSP) produced from different optical sources (i.e. various color LEDs, 3 mW diode laser, and 10mW He-Ne laser) with different produced operating surfaces (Gabor hologram diffusers), and how they affects the performance of the optical imaging systems; speckle size and signal-to-noise ratio (signal is represented by the patches of the speckles that contain or carry information, and noise is represented by the whole remaining part of the selected image). The theoretical and experimental studies of the colorimetry (color correction is done in the color images captured by the optical imaging system to produce realistic color images which contains most of the information in the image by selecting suitable gray scale which contains most of the informative data in the image, this is done by calculating the accurate Red-Green-Blue (RGB) color components making use of the measured spectrum for light sources, and color matching functions of International Telecommunication Organization (ITU-R709) for CRT phosphorus, Tirinton-SONY Model ) for the used optical sources are investigated and introduced to present the relations between the signal-to-noise ratios with different diffusers for each light source. The source surface coupling has been discussed and concludes that the performance of the optical imaging system for certain source varies from worst to best based on the operating surface. The sensor /surface coupling has been studied and discussed for the case of He-Ne laser and concludes the speckle size is ranged from 4.59 to 4.62 μm, which are slightly different or approximately the same for all produced diffusers (which satisfies the fact that the speckle size is independent on the illuminating surface). But, the calculated value of signal-tonoise ratio takes different values ranged from 0.71 to 0.92 for different diffuser. This means that the surface texture affects the performance of the optical sensor because, all images captured for all diffusers under the same conditions [same source (He-Ne laser), same distances of the experimental set-up, and the same sensor (CCD camera)].

Holographic analysis as an inspection method for welded thin-wall tubing

NASA Technical Reports Server (NTRS)

Brooks, Lawrence; Mulholland, John; Genin, Joseph; Matthews, Larryl

1990-01-01

The feasibility of using holographic interferometry for locating flaws in welded tubing is explored. Two holographic techniques are considered: traditional holographic interferometry and electronic speckle pattern interferometry. Several flaws including cold laps, discontinuities, and tube misalignments are detected.

Use of Laser Speckle Contrast Imaging to Assess Digital Microvascular Function in Primary Raynaud Phenomenon and Systemic Sclerosis: A Comparison Using the Raynaud Condition Score Diary.

PubMed

Pauling, John D; Shipley, Jacqueline A; Hart, Darren J; McGrogan, Anita; McHugh, Neil J

2015-07-01

Evaluate objective assessment of digital microvascular function using laser speckle contrast imaging (LSCI) in a cross-sectional study of patients with primary Raynaud phenomenon (RP) and systemic sclerosis (SSc), comparing LSCI with both infrared thermography (IRT) and subjective assessment using the Raynaud Condition Score (RCS) diary. Patients with SSc (n = 25) and primary RP (n = 18) underwent simultaneous assessment of digital perfusion using LSCI and IRT with a cold challenge on 2 occasions, 2 weeks apart. The RCS diary was completed between assessments. The relationship between objective and subjective assessments of RP was evaluated. Reproducibility of LSCI/IRT was assessed, along with differences between primary RP and SSc, and the effect of sex. There was moderate-to-good correlation between LSCI and IRT (Spearman rho 0.58-0.84, p < 0.01), but poor correlation between objective assessments and the RCS diary (p > 0.05 for all analyses). Reproducibility of IRT and LSCI was moderate at baseline (ICC 0.51-0.63) and immediately following cold challenge (ICC 0.56-0.86), but lower during reperfusion (ICC 0.3-0.7). Neither subjective nor objective assessments differentiated between primary RP and SSc. Men reported lower median daily frequency of RP attacks (0.82 vs 1.93, p = 0.03). Perfusion using LSCI/IRT was higher in men for the majority of assessments. Objective and subjective methods provide differing information on microvascular function in RP. There is good convergent validity of LSCI with IRT and acceptable reproducibility of both modalities. Neither subjective nor objective assessments could differentiate between primary RP and SSc. Influence of sex on subjective and objective assessment of RP warrants further evaluation.

URSI and Nachrichtentechnische Gesellschaft, General Session, Kleinheubach, West Germany, Oct. 6-10, 1986, Reports

NASA Astrophysics Data System (ADS)

Factors affecting the atmospheric propagation of EM waves, research on the ionosphere, and advances in radar and communications technology are examined in reviews and reports. Topics discussed include refraction corrections for radio astronomy and geodesy, speckle masking, radar studies of atmospheric motion, EISCAT measurements in the polar electrojet, active experiments in the polar ionosphere, and dispersion relations for drift-Alfven and drift-acoustic waves. Consideration is given to a microcomputer prediction system for HF communications over Europe, frequency determination of a hyperfine line of CH4 at 88 THz, multipath propagation in digital mobile communication, a robust digital voice transmission technique for land mobile radio, CMOS LSI for digital signal processing in mobile radio equipment, the representation of EM fields by dyadic Green functions, scalarization of Maxwell's equations for anisotropic media, and satellite antennas for land vehicles and aircraft.

Intraventricular Flow Velocity Vector Visualization Based on the Continuity Equation and Measurements of Vorticity and Wall Shear Stress

NASA Astrophysics Data System (ADS)

Itatani, Keiichi; Okada, Takashi; Uejima, Tokuhisa; Tanaka, Tomohiko; Ono, Minoru; Miyaji, Kagami; Takenaka, Katsu

2013-07-01

We have developed a system to estimate velocity vector fields inside the cardiac ventricle by echocardiography and to evaluate several flow dynamical parameters to assess the pathophysiology of cardiovascular diseases. A two-dimensional continuity equation was applied to color Doppler data using speckle tracking data as boundary conditions, and the velocity component perpendicular to the echo beam line was obtained. We determined the optimal smoothing method of the color Doppler data, and the 8-pixel standard deviation of the Gaussian filter provided vorticity without nonphysiological stripe shape noise. We also determined the weight function at the bilateral boundaries given by the speckle tracking data of the ventricle or vascular wall motion, and the weight function linear to the distance from the boundary provided accurate flow velocities not only inside the vortex flow but also around near-wall regions on the basis of the results of the validation of a digital phantom of a pipe flow model.

VizieR Online Data Catalog: Accurate astrometry & RVs of 4 multiple systems (Tokovinin+, 2017)

NASA Astrophysics Data System (ADS)

Tokovinin, A.; Latham, D. W.

2017-10-01

The outer subsystems are classical visual binaries. Historic micrometric measurements and modern speckle interferometric data have been obtained from the WDS database on our request. Additionally, we secured new speckle astrometry and relative photometry of two systems at the 4.1m SOAR telescope. Published radial velocities (RVs) are used here together with the new data. The RVs were measured with the CfA Digital Speedometers, initially using the 1.5m Wyeth Reflector at the Oak Ridge Observatory in the town of Harvard, Massachusetts, and subsequently with the 1.5m Tillinghast Reflector at the Whipple Observatory on Mount Hopkins, Arizona. Starting in 2009, the new fiber-fed Tillinghast Reflector Echelle Spectrograph (TRES) was used. The spectral resolution was 44000 for all three spectrographs. Two objects, HIP 101955 and 103987, were observed in 2015 with the CHIRON echelle spectrograph at the 1.5m telescope at CTIO with a spectral resolution of 80000. (4 data files).

Speckle field as a multiple particle trap

NASA Astrophysics Data System (ADS)

Shvedov, V. G.; Rode, A. V.; Izdebskaya, Ya. V.; Desyatnikov, A. S.; Krolikowski, W.; Kivshar, Yu. S.

2010-04-01

We demonstrate that a speckle pattern in the spatially coherent laser field transmitted by a diffuser forms a multitude of three-dimensional bottle-shaped micro-traps. These multiple traps serve as a means for an effective trapping of large number of air-born absorbing particles. Confinement of up to a few thousand particles in air with a single beam has been achieved. The ability to capture light-absorbing particles suspended in gases by optical means opens up rich and diverse practical opportunities, including development of photonic shielding/fencing for environmental protection in nanotechnology industry and new methods of touch-free air transport of particles and small containers, which may hold dangerous substances, or viruses and living cells.

Wavefront sensing with a thin diffuser

NASA Astrophysics Data System (ADS)

Berto, Pascal; Rigneault, Hervé; Guillon, Marc

2017-12-01

We propose and implement a broadband, compact, and low-cost wavefront sensing scheme by simply placing a thin diffuser in the close vicinity of a camera. The local wavefront gradient is determined from the local translation of the speckle pattern. The translation vector map is computed thanks to a fast diffeomorphic image registration algorithm and integrated to reconstruct the wavefront profile. The simple translation of speckle grains under local wavefront tip/tilt is ensured by the so-called "memory effect" of the diffuser. Quantitative wavefront measurements are experimentally demonstrated both for the few first Zernike polynomials and for phase-imaging applications requiring high resolution. We finally provided a theoretical description of the resolution limit that is supported experimentally.

Digital adaptive optics line-scanning confocal imaging system.

PubMed

Liu, Changgeng; Kim, Myung K

2015-01-01

A digital adaptive optics line-scanning confocal imaging (DAOLCI) system is proposed by applying digital holographic adaptive optics to a digital form of line-scanning confocal imaging system. In DAOLCI, each line scan is recorded by a digital hologram, which allows access to the complex optical field from one slice of the sample through digital holography. This complex optical field contains both the information of one slice of the sample and the optical aberration of the system, thus allowing us to compensate for the effect of the optical aberration, which can be sensed by a complex guide star hologram. After numerical aberration compensation, the corrected optical fields of a sequence of line scans are stitched into the final corrected confocal image. In DAOLCI, a numerical slit is applied to realize the confocality at the sensor end. The width of this slit can be adjusted to control the image contrast and speckle noise for scattering samples. DAOLCI dispenses with the hardware pieces, such as Shack–Hartmann wavefront sensor and deformable mirror, and the closed-loop feedbacks adopted in the conventional adaptive optics confocal imaging system, thus reducing the optomechanical complexity and cost. Numerical simulations and proof-of-principle experiments are presented that demonstrate the feasibility of this idea.

Speckle perception and disturbance limit in laser based projectors

NASA Astrophysics Data System (ADS)

Verschaffelt, Guy; Roelandt, Stijn; Meuret, Youri; Van den Broeck, Wendy; Kilpi, Katriina; Lievens, Bram; Jacobs, An; Janssens, Peter; Thienpont, Hugo

2016-04-01

We investigate the level of speckle that can be tolerated in a laser cinema projector. For this purpose, we equipped a movie theatre room with a prototype laser projector. A group of 186 participants was gathered to evaluate the speckle perception of several, short movie trailers in a subjective `Quality of Experience' experiment. This study is important as the introduction of lasers in projection systems has been hampered by the presence of speckle in projected images. We identify a speckle disturbance threshold by statistically analyzing the observers' responses for different values of the amount of speckle, which was monitored using a well-defined speckle measurement method. The analysis shows that the speckle perception of a human observer is not only dependent on the objectively measured amount of speckle, but it is also strongly influenced by the image content. As is also discussed in [Verschaffelt et al., Scientific Reports 5, art. nr. 14105, 2015] we find that, for moving images, the speckle becomes disturbing if the speckle contrast becomes larger than 6.9% for the red, 6.0% for the green, and 4.8% for the blue primary colors of the projector, whereas for still images the speckle detection threshold is about 3%. As we could not independently tune the speckle contrast of each of the primary colors, this speckle disturbance limit seems to be determined by the 6.9% speckle contrast of the red color as this primary color contains the largest amount of speckle. The speckle disturbance limit for movies thus turns out to be substantially larger than that for still images, and hence is easier to attain.

Human speckle perception threshold for still images from a laser projection system.

PubMed

Roelandt, Stijn; Meuret, Youri; Jacobs, An; Willaert, Koen; Janssens, Peter; Thienpont, Hugo; Verschaffelt, Guy

2014-10-06

We study the perception of speckle by human observers in a laser projector based on a 40 persons survey. The speckle contrast is first objectively measured making use of a well-defined speckle measurement method. We statistically analyse the results of the user quality scores, revealing that the speckle perception is not only influenced by the speckle contrast settings of the projector, but it is also strongly influenced by the type of image shown. Based on the survey, we derive a speckle contrast threshold for which speckle can be seen, and separately we investigate a speckle disturbance limit that is tolerated by the majority of test persons.

Development and testing of 2-dimensional photon counter

NASA Technical Reports Server (NTRS)

1981-01-01

The development of a commercially available two dimensional photon counter into an operational system for speckle imaging of astronomical objects is described. The system includes digital recording for field observations. The counter has a bialkali photocathode with a field size of 18 by 18 mm over which it resolves about 100 by 100 pixels. The system records photon positions as 16 bit words at rates up to 14,400 per second. Field tests at observatories verifying the operation of the system are described.

Adaptive box filters for removal of random noise from digital images

USGS Publications Warehouse

Eliason, E.M.; McEwen, A.S.

1990-01-01

We have developed adaptive box-filtering algorithms to (1) remove random bit errors (pixel values with no relation to the image scene) and (2) smooth noisy data (pixels related to the image scene but with an additive or multiplicative component of noise). For both procedures, we use the standard deviation (??) of those pixels within a local box surrounding each pixel, hence they are adaptive filters. This technique effectively reduces speckle in radar images without eliminating fine details. -from Authors

A new product for photon-limited imaging

NASA Astrophysics Data System (ADS)

Gonsiorowski, Thomas

1986-01-01

A new commercial low-light imaging detector, the Photon Digitizing Camera (PDC), is based on the PAPA detector developed at Harvard University. The PDC generates (x, y, t)-coordinate data of each detected photoevent. Because the positional address computation is performed optically, very high counting rates are achieved even at full spatial resolution. Careful optomechanical and electronic design results in a compact, rugged detector with superb performance. The PDC will be used for speckle imaging of astronomical sources and other astronomical and low-light applications.

Analysis of statistical properties of laser speckles, forming in skin and mucous of colon: potential application in laser surgery

NASA Astrophysics Data System (ADS)

Rubtsov, Vladimir; Kapralov, Sergey; Chalyk, Iuri; Ulianova, Onega; Ulyanov, Sergey

2013-02-01

Statistical properties of laser speckles, formed in skin and mucous of colon have been analyzed and compared. It has been demonstrated that first and second order statistics of "skin" speckles and "mucous" speckles are quite different. It is shown that speckles, formed in mucous, are not Gaussian one. Layered structure of colon mucous causes formation of speckled biospeckles. First- and second- order statistics of speckled speckles have been reviewed in this paper. Statistical properties of Fresnel and Fraunhofer doubly scattered and cascade speckles are described. Non-gaussian statistics of biospeckles may lead to high localization of intensity of coherent light in human tissue during the laser surgery. Way of suppression of highly localized non-gaussian speckles is suggested.

Anti-dsDNA antibodies in systemic lupus erythematosus: A combination of two quantitative methods and the ANA pattern is the most efficient strategy of detection.

PubMed

Almeida González, Delia; Roces Varela, Alfredo; Marcelino Rodríguez, Itahisa; González Vera, Alexander; Delgado Sánchez, Mónica; Aznar Esquivel, Antonio; Casañas Rodríguez, Carlos; Cabrera de León, Antonio

2015-12-01

Several methods have been used to measure anti-double-stranded DNA auto-antibody (anti-dsDNA). Our aim was to determine the most efficient strategy to test anti-dsDNA in systemic lupus erythematosus (SLE). In this study, anti-dsDNA and anti-nuclear antibody (ANA) tests were requested for 644 patients. Anti-dsDNA was tested by RIA, ELISA and CLIA in all patients. The results indicated that 78 patients had a positive anti-dsDNA test according to at least one of the methods. After a 3-year follow-up period only 26 patients were diagnosed with SLE. We evaluated each method and combination of methods. Specificity and positive predictive value (PPV) increased with the number of assay methods used (p=0.002 for trend), and PPV was 100% in patients whose results were positive by all three anti-dsDNA assay methods. The proportion of anti-dsDNA-positive patients who had SLE was highest (82%; p b 0.001) among those with a homogeneous pattern of ANA staining, followed by those with a speckled pattern. In ANA positive patients, when only RIA was considered, 59% of anti-dsDNA-positive patients had SLE, but when RIA and CLIA were both considered, all patients with positive results on both tests had SLE. The combination of RIA+CLIA in patients with homogeneous and speckled ANA staining showed a similar cost and higher sensitivity than RIA alone in ANA positive patients (p b 0.001). We conclude that the most efficient strategy was to combine simultaneously two quantitative and sensitive methods but only in patients with a homogeneous or speckled pattern of ANA staining. This approach maximized specificity and PPV, and reduced costs. Copyright © 2015 Elsevier B.V. All rights reserved.

Multi-layered bird beaks: a finite-element approach towards the role of keratin in stress dissipation

PubMed Central

Soons, Joris; Herrel, Anthony; Genbrugge, Annelies; Adriaens, Dominique; Aerts, Peter; Dirckx, Joris

2012-01-01

Bird beaks are layered structures, which contain a bony core and an outer keratin layer. The elastic moduli of this bone and keratin were obtained in a previous study. However, the mechanical role and interaction of both materials in stress dissipation during seed crushing remain unknown. In this paper, a multi-layered finite-element (FE) model of the Java finch's upper beak (Padda oryzivora) is established. Validation measurements are conducted using in vivo bite forces and by comparing the displacements with those obtained by digital speckle pattern interferometry. Next, the Young modulus of bone and keratin in this FE model was optimized in order to obtain the smallest peak von Mises stress in the upper beak. To do so, we created a surrogate model, which also allows us to study the impact of changing material properties of both tissues on the peak stresses. The theoretically best values for both moduli in the Java finch are retrieved and correspond well with previous experimentally obtained values, suggesting that material properties are tuned to the mechanical demands imposed during seed crushing. PMID:22337628

In situ strain and temperature measurement and modelling during arc welding

DOE PAGES

Chen, Jian; Yu, Xinghua; Miller, Roger G.; ...

2014-12-26

In this study, experiments and numerical models were applied to investigate the thermal and mechanical behaviours of materials adjacent to the weld pool during arc welding. In the experiment, a new high temperature strain measurement technique based on digital image correlation (DIC) was developed and applied to measure the in situ strain evolution. In contrast to the conventional DIC method that is vulnerable to the high temperature and intense arc light involved in fusion welding processes, the new technique utilised a special surface preparation method to produce high temperature sustaining speckle patterns required by the DIC algorithm as well asmore » a unique optical illumination and filtering system to suppress the influence of the intense arc light. These efforts made it possible for the first time to measure in situ the strain field 1 mm away from the fusion line. The temperature evolution in the weld and the adjacent regions was simultaneously monitored by an infrared camera. Finally and additionally, a thermal–mechanical finite element model was applied to substantiate the experimental measurement.« less

The effect of hydrogen embrittlement on the localized plastic deformation of aluminum alloy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bochkareva, Anna, E-mail: avb@ispms.tsc.ru; Lunev, Aleksey, E-mail: agl@ispms.tsc.ru; National Research Tomsk Polytechnic University, Tomsk, 634050

2015-10-27

The effect of hydrogen embrittlement on the localized plastic deformation of aluminum alloy D1 was investigated. The studies were performed for the test samples of aluminum alloy subjected to electrolytic hydrogenation. It is found that the mechanical properties and localized plastic deformation parameters of aluminum alloy are affected adversely by hydrogen embrittlement. The hydrogenated counterpart of alloy has a lower degree of ductility relative to the original alloy; however, the plastic flow behavior of material remains virtually unaffected. Using scanning electron and atomic force microscopy methods, the changes in the fracture surface were investigated. The deformation diagrams were examined formore » the deformed samples of aluminum alloy. These are found to show all the plastic flow stages: the linear, parabolic and pre-failure stages would occur for the respective values of the exponent n from the Ludwik-Holomon equation. Using digital speckle image technique, the local strain patterns were being registered for the original alloy D1 and the counterpart subjected to electrolytic hydrogenation for 100 h.« less

Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds

PubMed Central

Hajjarian, Zeinab; Nia, Hadi Tavakoli; Ahn, Shawn; Grodzinsky, Alan J.; Jain, Rakesh K.; Nadkarni, Seemantini K.

2016-01-01

Natural and synthetic hydrogel scaffolds exhibit distinct viscoelastic properties at various length scales and deformation rates. Laser Speckle Rheology (LSR) offers a novel, non-contact optical approach for evaluating the frequency-dependent viscoelastic properties of hydrogels. In LSR, a coherent laser beam illuminates the specimen and a high-speed camera acquires the time-varying speckle images. Cross-correlation analysis of frames returns the speckle intensity autocorrelation function, g2(t), from which the frequency-dependent viscoelastic modulus, G*(ω), is deduced. Here, we establish the capability of LSR for evaluating the viscoelastic properties of hydrogels over a large range of moduli, using conventional mechanical rheometry and atomic force microscopy (AFM)-based indentation as reference-standards. Results demonstrate a strong correlation between |G*(ω)| values measured by LSR and mechanical rheometry (r = 0.95, p < 10−9), and z-test analysis reports that moduli values measured by the two methods are identical (p > 0.08) over a large range (47 Pa – 36 kPa). In addition, |G*(ω)| values measured by LSR correlate well with indentation moduli, E, reported by AFM (r = 0.92, p < 10−7). Further, spatially-resolved moduli measurements in micro-patterned substrates demonstrate that LSR combines the strengths of conventional rheology and micro-indentation in assessing hydrogel viscoelastic properties at multiple frequencies and small length-scales. PMID:27905494

Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds.

PubMed

Hajjarian, Zeinab; Nia, Hadi Tavakoli; Ahn, Shawn; Grodzinsky, Alan J; Jain, Rakesh K; Nadkarni, Seemantini K

2016-12-01

Natural and synthetic hydrogel scaffolds exhibit distinct viscoelastic properties at various length scales and deformation rates. Laser Speckle Rheology (LSR) offers a novel, non-contact optical approach for evaluating the frequency-dependent viscoelastic properties of hydrogels. In LSR, a coherent laser beam illuminates the specimen and a high-speed camera acquires the time-varying speckle images. Cross-correlation analysis of frames returns the speckle intensity autocorrelation function, g 2 (t), from which the frequency-dependent viscoelastic modulus, G*(ω), is deduced. Here, we establish the capability of LSR for evaluating the viscoelastic properties of hydrogels over a large range of moduli, using conventional mechanical rheometry and atomic force microscopy (AFM)-based indentation as reference-standards. Results demonstrate a strong correlation between |G*(ω)| values measured by LSR and mechanical rheometry (r = 0.95, p < 10 -9 ), and z-test analysis reports that moduli values measured by the two methods are identical (p > 0.08) over a large range (47 Pa - 36 kPa). In addition, |G*(ω)| values measured by LSR correlate well with indentation moduli, E, reported by AFM (r = 0.92, p < 10 -7 ). Further, spatially-resolved moduli measurements in micro-patterned substrates demonstrate that LSR combines the strengths of conventional rheology and micro-indentation in assessing hydrogel viscoelastic properties at multiple frequencies and small length-scales.

Verification of micro-scale photogrammetry for smooth three-dimensional object measurement

NASA Astrophysics Data System (ADS)

Sims-Waterhouse, Danny; Piano, Samanta; Leach, Richard

2017-05-01

By using sub-millimetre laser speckle pattern projection we show that photogrammetry systems are able to measure smooth three-dimensional objects with surface height deviations less than 1 μm. The projection of laser speckle patterns allows correspondences on the surface of smooth spheres to be found, and as a result, verification artefacts with low surface height deviations were measured. A combination of VDI/VDE and ISO standards were also utilised to provide a complete verification method, and determine the quality parameters for the system under test. Using the proposed method applied to a photogrammetry system, a 5 mm radius sphere was measured with an expanded uncertainty of 8.5 μm for sizing errors, and 16.6 μm for form errors with a 95 % confidence interval. Sphere spacing lengths between 6 mm and 10 mm were also measured by the photogrammetry system, and were found to have expanded uncertainties of around 20 μm with a 95 % confidence interval.

Analysis of microvascular perfusion with multi-dimensional complete ensemble empirical mode decomposition with adaptive noise algorithm: Processing of laser speckle contrast images recorded in healthy subjects, at rest and during acetylcholine stimulation.

PubMed

Humeau-Heurtier, Anne; Marche, Pauline; Dubois, Severine; Mahe, Guillaume

2015-01-01

Laser speckle contrast imaging (LSCI) is a full-field imaging modality to monitor microvascular blood flow. It is able to give images with high temporal and spatial resolutions. However, when the skin is studied, the interpretation of the bidimensional data may be difficult. This is why an averaging of the perfusion values in regions of interest is often performed and the result is followed in time, reducing the data to monodimensional time series. In order to avoid such a procedure (that leads to a loss of the spatial resolution), we propose to extract patterns from LSCI data and to compare these patterns for two physiological states in healthy subjects: at rest and at the peak of acetylcholine-induced perfusion peak. For this purpose, the recent multi-dimensional complete ensemble empirical mode decomposition with adaptive noise (MCEEMDAN) algorithm is applied to LSCI data. The results show that the intrinsic mode functions and residue given by MCEEMDAN show different patterns for the two physiological states. The images, as bidimensional data, can therefore be processed to reveal microvascular perfusion patterns, hidden in the images themselves. This work is therefore a feasibility study before analyzing data in patients with microvascular dysfunctions.

Analysis of a multi-wavelength multi-camera phase-shifting profilometric system for real-time operation

NASA Astrophysics Data System (ADS)

Stoykova, Elena; Gotchev, Atanas; Sainov, Ventseslav

2011-01-01

Real-time accomplishment of a phase-shifting profilometry through simultaneous projection and recording of fringe patterns requires a reliable phase retrieval procedure. In the present work we consider a four-wavelength multi-camera system with four sinusoidal phase gratings for pattern projection that implements a four-step algorithm. Successful operation of the system depends on overcoming two challenges which stem out from the inherent limitations of the phase-shifting algorithm, namely the demand for a sinusoidal fringe profile and the necessity to ensure equal background and contrast of fringes in the recorded fringe patterns. As a first task, we analyze the systematic errors due to the combined influence of the higher harmonics and multi-wavelength illumination in the Fresnel diffraction zone considering the case when the modulation parameters of the four gratings are different. As a second task we simulate the system performance to evaluate the degrading effect of the speckle noise and the spatially varying fringe modulation at non-uniform illumination on the overall accuracy of the profilometric measurement. We consider the case of non-correlated speckle realizations in the recorded fringe patterns due to four-wavelength illumination. Finally, we apply a phase retrieval procedure which includes normalization, background removal and denoising of the recorded fringe patterns to both simulated and measured data obtained for a dome surface.

Remote sensing of atmospheric winds using a coherent, CW lidar and speckle-turbulence interaction

NASA Technical Reports Server (NTRS)

Holmes, J. F.; Amzajerdian, F.; Gudimetla, V. S. R.; Hunt, J. M.

1986-01-01

Speckle turbulence interaction has the potential for allowing single ended remote sensing of the path averaged vector crosswind in a plane perpendicular to the line of sight to a target. If a laser transmitter is used to illuminate a target, the resultant speckle field generated by the target is randomly perturbed by the atmospheric turbulence as it propagates back to the location of the transmitter-receiver. When a cross wind is present, this scintillation pattern will move with time across the receiver. A continuous wave (cw) laser transmitter of modest power level in conjunction with optical heterodyne detection was used to exploit the speckel turbulence interaction and measure the crosswind. The use of a cw transmitter at 10.6 microns and optical heterodyne detection has many advantages over direct detection and a double pulsed source in the visible or near infrared. These advantages include the availability of compact, reliable and inexpensive transmitters, better penetration of smoke, dust and fog; stable output power; low beam pointing jitter; and considerably reduced complexity in the receiver electronics.

Adaptive mesh optimization and nonrigid motion recovery based image registration for wide-field-of-view ultrasound imaging.

PubMed

Tan, Chaowei; Wang, Bo; Liu, Paul; Liu, Dong

2008-01-01

Wide field of view (WFOV) imaging mode obtains an ultrasound image over an area much larger than the real time window normally available. As the probe is moved over the region of interest, new image frames are combined with prior frames to form a panorama image. Image registration techniques are used to recover the probe motion, eliminating the need for a position sensor. Speckle patterns, which are inherent in ultrasound imaging, change, or become decorrelated, as the scan plane moves, so we pre-smooth the image to reduce the effects of speckle in registration, as well as reducing effects from thermal noise. Because we wish to track the movement of features such as structural boundaries, we use an adaptive mesh over the entire smoothed image to home in on areas with feature. Motion estimation using blocks centered at the individual mesh nodes generates a field of motion vectors. After angular correction of motion vectors, we model the overall movement between frames as a nonrigid deformation. The polygon filling algorithm for precise, persistence-based spatial compounding constructs the final speckle reduced WFOV image.

Low Dose X-Ray Speckle Visibility Spectroscopy Reveals Nanoscale Dynamics in Radiation Sensitive Ionic Liquids

NASA Astrophysics Data System (ADS)

Verwohlt, Jan; Reiser, Mario; Randolph, Lisa; Matic, Aleksandar; Medina, Luis Aguilera; Madsen, Anders; Sprung, Michael; Zozulya, Alexey; Gutt, Christian

2018-04-01

X-ray radiation damage provides a serious bottleneck for investigating microsecond to second dynamics on nanometer length scales employing x-ray photon correlation spectroscopy. This limitation hinders the investigation of real time dynamics in most soft matter and biological materials which can tolerate only x-ray doses of kGy and below. Here, we show that this bottleneck can be overcome by low dose x-ray speckle visibility spectroscopy. Employing x-ray doses of 22-438 kGy and analyzing the sparse speckle pattern of count rates as low as 6.7 ×10-3 per pixel, we follow the slow nanoscale dynamics of an ionic liquid (IL) at the glass transition. At the prepeak of nanoscale order in the IL, we observe complex dynamics upon approaching the glass transition temperature TG with a freezing in of the alpha relaxation and a multitude of millisecond local relaxations existing well below TG . We identify this fast relaxation as being responsible for the increasing development of nanoscale order observed in ILs at temperatures below TG .

Optical characterization of display screens by speckle-contrast measurements

NASA Astrophysics Data System (ADS)

Pozo, Antonio M.; Castro, José J.; Rubiño, Manuel

2012-10-01

In recent years, the flat-panel display (FPD) technology has undergone great development. Currently, FPDs are present in many devices. A significant element in FPD manufacturing is the display front surface. Manufacturers sell FPDs with different types of front surface which can be matte (also called anti-glare) or glossy screens. Users who prefer glossy screens consider images shown in these types of displays to have more vivid colours compared with matte-screen displays. However, external light sources may cause unpleasant reflections on the glossy screens. These reflections can be reduced by a matte treatment in the front surface of FPDs. In this work, we present a method to characterize the front surface of FPDs using laser speckle patterns. We characterized three FPDs: a Samsung XL2370 LCD monitor of 23" with matte screen, a Toshiba Satellite A100 laptop of 15.4" with glossy screen, and a Papyre electronic book reader. The results show great differences in speckle contrast values for the three screens characterized and, therefore, this work shows the feasibility of this method for characterizing and comparing FPDs which have different types of front surfaces.

Speckle reduction using deformable mirrors with diffusers in a laser pico-projector.

PubMed

Chen, Hsuan-An; Pan, Jui-Wen; Yang, Zu-Po

2017-07-24

We propose a design for speckle reduction in a laser pico-projector adopting diffusers and deformable mirrors. This research focuses on speckle noise suppression by changing the angle of divergence of the diffuser. Moreover, the speckle contrast value can be further reduced by the addition of a deformable mirror. The speckle reduction ability obtained using diffusers with different divergence angles is compared. Three types of diffuser designs are compared in the experiments. For Type 1 which uses a circular symmetric diffuser the speckle contrast value can be decreased to 0.0264. For Type 2, the speckle contrast value can be reduced to 0.0267 because of the inclusion of an elliptical distribution diffuser. With Type 3 which includes a combination of the circular distribution diffuser and elliptical distribution diffuser, the speckle contrast value can be reduced to 0.0236. For all three types, the speckle contrast value is lower than 0.05. Under this speckle value, the speckle phenomenon is invisible to the human eye.

Speckle measuring instrument based on biological characteristics of the human eyes and speckle reduction with advanced electromagnetic micro-scanning mirror

NASA Astrophysics Data System (ADS)

Yuan, Yuan; Fang, Tao; Sun, Min Yuan; Gao, Wei Nan; Zhang, Shuo; Bi, Yong

2018-07-01

Laser speckle is a major issue for laser projection displays. In various techniques of speckle reduction, speckle is quantified with a speckle contrast value. However, the measured speckle contrast is poorly suited for the subjective speckle perception of a human observer. Here, we investigate the characteristics of human eyes and propose a simplified optical transfer function of human eyes. Accordingly, two human-eye-modeled speckle measuring sets are configured. Based on the experimental set, an advanced electromagnetic micro-scanning mirror (EM-MSM) is exploited; which is of 6.5 mm in diameter and its half angle is 7.8° for a horizontal scan and 6.53° for a vertical scan. Finally, we quantitatively show that images generated with an EM-MSM exhibit superior quality. By providing human-eye-modeled speckle measuring instruments and an EM-MSM for speckle reduction, it has a promising promotion to laser projector development.

A shape-preserving oriented partial differential equation based on a new fidelity term for electronic speckle pattern interferometry fringe patterns denoising

NASA Astrophysics Data System (ADS)

Xu, Wenjun; Tang, Chen; Zheng, Tingyue; Qiu, Yue

2018-07-01

Oriented partial differential equations (OPDEs) have been demonstrated to be a powerful tool for preserving the integrity of fringes while filtering electronic speckle pattern interferometry (ESPI) fringe patterns. However, the main drawback of OPDEs-based methods is that many iterations are often needed, which causes the change in the shape of fringes. Change in the shape of fringes will affect the accuracy of subsequent fringe analysis. In this paper, we focus on preserving the shape of fringes while filtering, suggested here for the first time. We propose a shape-preserving OPDE for ESPI fringe patterns denoising by introducing a new fidelity term to the previous second-order single oriented PDE (SOOPDE). In our proposed fidelity term, the evolution image is subtracted from the shrinkage result of original noisy image by shearlet transform. Our proposed shape-preserving OPDE is capable of eliminating noise effectively, keeping the integrity of fringes, and more importantly, preserving the shape of fringes. We test the proposed shape-preserving OPDE on three computer-simulated and three experimentally obtained ESPI fringe patterns with poor quality. Furthermore, we compare our model with three representative filtering methods, including the widely used SOOPDE, shearlet transform and coherence-enhancing diffusion (CED). We also compare our proposed fidelity term with the traditional fidelity term. Experimental results show that the proposed shape-preserving OPDE not only yields filtered images with visual quality on par with those by CED which is the state-of-the-art method for ESPI fringe patterns denoising, but also keeps the shape of ESPI fringe patterns.

Caustics and Rogue Waves in an Optical Sea.

PubMed

Mathis, Amaury; Froehly, Luc; Toenger, Shanti; Dias, Frédéric; Genty, Goëry; Dudley, John M

2015-08-06

There are many examples in physics of systems showing rogue wave behaviour, the generation of high amplitude events at low probability. Although initially studied in oceanography, rogue waves have now been seen in many other domains, with particular recent interest in optics. Although most studies in optics have focussed on how nonlinearity can drive rogue wave emergence, purely linear effects have also been shown to induce extreme wave amplitudes. In this paper, we report a detailed experimental study of linear rogue waves in an optical system, using a spatial light modulator to impose random phase structure on a coherent optical field. After free space propagation, different random intensity patterns are generated, including partially-developed speckle, a broadband caustic network, and an intermediate pattern with characteristics of both speckle and caustic structures. Intensity peaks satisfying statistical criteria for rogue waves are seen especially in the case of the caustic network, and are associated with broader spatial spectra. In addition, the electric field statistics of the intermediate pattern shows properties of an "optical sea" with near-Gaussian statistics in elevation amplitude, and trough-to-crest statistics that are near-Rayleigh distributed but with an extended tail where a number of rogue wave events are observed.

Caustics and Rogue Waves in an Optical Sea

PubMed Central

Mathis, Amaury; Froehly, Luc; Toenger, Shanti; Dias, Frédéric; Genty, Goëry; Dudley, John M.

2015-01-01

There are many examples in physics of systems showing rogue wave behaviour, the generation of high amplitude events at low probability. Although initially studied in oceanography, rogue waves have now been seen in many other domains, with particular recent interest in optics. Although most studies in optics have focussed on how nonlinearity can drive rogue wave emergence, purely linear effects have also been shown to induce extreme wave amplitudes. In this paper, we report a detailed experimental study of linear rogue waves in an optical system, using a spatial light modulator to impose random phase structure on a coherent optical field. After free space propagation, different random intensity patterns are generated, including partially-developed speckle, a broadband caustic network, and an intermediate pattern with characteristics of both speckle and caustic structures. Intensity peaks satisfying statistical criteria for rogue waves are seen especially in the case of the caustic network, and are associated with broader spatial spectra. In addition, the electric field statistics of the intermediate pattern shows properties of an “optical sea” with near-Gaussian statistics in elevation amplitude, and trough-to-crest statistics that are near-Rayleigh distributed but with an extended tail where a number of rogue wave events are observed. PMID:26245864

Using of dynamic speckled speckles with a small number of scatterers for study of suspension of Chlamydia

NASA Astrophysics Data System (ADS)

Ulyanov, Sergey; Filonova, Nadezhda; Ulianova, Onega; Utz, Sergey; Moiseeva, Yulia; Subbotina, Irina; Kalduzova, Irina; Larionova, Olga; Feodorova, Valentina

2018-04-01

Theory of formation of speckled speckles at diffraction of focused Gaussian beam in the suspension, containing of Chlamydia trachomatis (CT) is presented. Optical model of scattering of light in suspension of Chlamydia is suggested. Formula for bandwidth of spectrum of intensity fluctuations in speckled speckles is derived. It has been demonstrated, that speckle-microscopy can be used for detection of CT bacteria for any concentration of the relevant cells in suspension.

Ultrasound Imaging of Muscle Contraction of the Tibialis Anterior in Patients with Facioscapulohumeral Dystrophy.

PubMed

Gijsbertse, Kaj; Goselink, Rianne; Lassche, Saskia; Nillesen, Maartje; Sprengers, André; Verdonschot, Nico; van Alfen, Nens; de Korte, Chris

2017-11-01

A need exists for biomarkers to diagnose, quantify and longitudinally follow facioscapulohumeral muscular dystrophy (FSHD) and many other neuromuscular disorders. Furthermore, the pathophysiological mechanisms leading to muscle weakness in most neuromuscular disorders are not completely understood. Dynamic ultrasound imaging (B-mode image sequences) in combination with speckle tracking is an easy, applicable and patient-friendly imaging tool to visualize and quantify muscle deformation. This dynamic information provides insight in the pathophysiological mechanisms and may help to distinguish the various stages of diseased muscle in FSHD. In this proof-of-principle study, we applied a speckle tracking technique to 2-D ultrasound image sequences to quantify the deformation of the tibialis anterior muscle in patients with FSHD and in healthy controls. The resulting deformation patterns were compared with muscle ultrasound echo intensity analysis (a measure of fat infiltration and dystrophy) and clinical outcome measures. Of the four FSHD patients, two patients had severe peroneal weakness and two patients had mild peroneal weakness on clinical examination. We found a markedly varied muscle deformation pattern between these groups: patients with severe peroneal weakness showed a different motion pattern of the tibialis anterior, with overall less displacement of the central tendon region, while healthy patients showed a non-uniform displacement pattern, with the central aponeurosis showing the largest displacement. Hence, dynamic muscle ultrasound of the tibialis anterior muscle in patients with FSHD revealed a distinctively different tissue deformation pattern among persons with and without tibialis anterior weakness. These findings could clarify the understanding of the pathophysiology of muscle weakness in FSHD patients. In addition, the change in muscle deformation shows good correlation with clinical measures and quantitative muscle ultrasound measurements. In conclusion, dynamic ultrasound in combination with speckle tracking allows the study of the effects of muscle pathology in relation to strength, force transmission and movement generation. Although further research is required, this technique can develop into a biomarker to quantify muscle disease severity. Copyright © 2017 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Serum antinuclear antibody in adult Thais.

PubMed

Prapinjumrune, Chanwit; Prucktrakul, Chalakorn; Sooktonglarng, Trakarn; Thongprasom, Kobkan

2017-03-01

This study investigated the presence of antinuclear antibody (ANA) in older Thais compared with middle-age and younger participants. Antinuclear antibody represents the first step in the diagnostic testing for lupus erythematosus (LE) and other autoimmune diseases. Due to the lack of reference ANA levels in older, middle-age and younger Thais healthy participants, this study will be useful for determining the proper diagnostic and treatment criteria. There were 28 older (60-76 years), 17 middle-age (41-59 years) and 13 younger (24-40 years) participants in this study. Immunofluorescence was performed to analyse the ANA staining pattern and titre levels in the participants' blood samples. The presence of serum ANA was found in 18 of 28 cases (64.3%), four of 17 (23.5%) and one of 13 cases (7.7%) of the older, middle-age and younger participants, respectively. The difference in the number of serum ANA-positive participants between the older, middle-age and younger groups was statistically significant (p < 0.05). Interestingly, the ANA positive in older participants presented more than one staining pattern. The speckled pattern was the most commonly detected ANA staining pattern in the older group, being found in 12 cases followed by cytoplasmic pattern (10 cases), homogeneous pattern (nine cases) and nucleolar pattern (five cases). In the middle-age group, the speckled pattern was found in four cases, whereas one younger participant presented a nucleolar pattern. Serum ANA positive was significantly higher in the older group compared with the middle-age and younger groups. There were variations of the serum ANA staining patterns in the older group. © 2016 John Wiley & Sons A/S and The Gerodontology Association. Published by John Wiley & Sons Ltd.

Demonstration of laser speckle system on burner liner cyclic rig

NASA Technical Reports Server (NTRS)

Stetson, K. A.

1986-01-01

A demonstration test was conducted to apply speckle photogrammetry to the measurement of strains on a sample of combustor liner material in a cyclic fatigue rig. A system for recording specklegrams was assembled and shipped to the NASA Lewis Research Center, where it was set up and operated during rig tests. Data in the form of recorded specklegrams were sent back to United Technologies Research Center for processing to extract strains. Difficulties were found in the form of warping and bowing of the sample during the tests which degraded the data. Steps were taken by NASA personnel to correct this problem and further tests were run. Final data processing indicated erratic patterns of strain on the burner liner sample.

Understanding the exposure-time effect on speckle contrast measurements for laser displays

NASA Astrophysics Data System (ADS)

Suzuki, Koji; Kubota, Shigeo

2018-02-01

To evaluate the influence of exposure time on speckle noise for laser displays, speckle contrast measurement method was developed observable at a human eye response time using a high-sensitivity camera which has a signal multiplying function. The nonlinearity of camera light sensitivity was calibrated to measure accurate speckle contrasts, and the measuring lower limit noise of speckle contrast was improved by applying spatial-frequency low pass filter to the captured images. Three commercially available laser displays were measured over a wide range of exposure times from tens of milliseconds to several seconds without adjusting the brightness of laser displays. The speckle contrast of raster-scanned mobile projector without any speckle-reduction device was nearly constant over various exposure times. On the contrary to this, in full-frame projection type laser displays equipped with a temporally-averaging speckle-reduction device, some of their speckle contrasts close to the lower limits noise were slightly increased at the shorter exposure time due to the noise. As a result, the exposure-time effect of speckle contrast could not be observed in our measurements, although it is more reasonable to think that the speckle contrasts of laser displays, which are equipped with the temporally-averaging speckle-reduction device, are dependent on the exposure time. This discrepancy may be attributed to the underestimation of temporal averaging factor. We expected that this method is useful for evaluating various laser displays and clarify the relationship between the speckle noise and the exposure time for a further verification of speckle reduction.

Correlation effects in focused transmission through disordered media (Conference Presentation)

NASA Astrophysics Data System (ADS)

Hsu, Chia Wei; Liew, Seng Fatt; Goetschy, Arthur; Cao, Hui; Stone, A. Douglas

2017-02-01

By controlling the many degrees of freedom in the incident wavefront, one can manipulate wave propagation in complex structures. Such wavefront-shaping methods have been used extensively for controlling light transmitted into wavelength-scale regions (speckles), a property that is insensitive to correlations in the speckle pattern. Extending coherent control to larger regions is of great interest both scientifically and for applications such as optical communications, photothermal therapy, and the imaging of large objects within or behind a diffusive medium. However, waves diffusing through a disordered medium are known to exhibit non-local intensity correlations, and their effect on coherent control has not been fully understood. Here, we demonstrate the effects of correlations with wavefront-shaping experiments on a scattering sample of zinc oxide microparticles. Long-range correlations substantially increase the dynamic range of coherent control over light transmitted onto larger target regions, far beyond what would be achievable if correlations were negligible. This and other effects of correlations emerge when the number of speckles targeted, M2, exceeds the dimensionless conductance g. Using a filtered random matrix ensemble appropriate for describing coherent diffusion and the lateral spreading in an open geometry, we show analytically that M2/g appears as the controlling parameter in universal scaling laws for several statistical properties of interest--predictions that we quantitatively confirm with experimental data. Our work elucidates the roles of speckle correlations and provides a general theoretical framework for modeling open systems in wavefront-shaping experiments.

Stochastic speckle noise compensation in optical coherence tomography using non-stationary spline-based speckle noise modelling.

PubMed

Cameron, Andrew; Lui, Dorothy; Boroomand, Ameneh; Glaister, Jeffrey; Wong, Alexander; Bizheva, Kostadinka

2013-01-01

Optical coherence tomography (OCT) allows for non-invasive 3D visualization of biological tissue at cellular level resolution. Often hindered by speckle noise, the visualization of important biological tissue details in OCT that can aid disease diagnosis can be improved by speckle noise compensation. A challenge with handling speckle noise is its inherent non-stationary nature, where the underlying noise characteristics vary with the spatial location. In this study, an innovative speckle noise compensation method is presented for handling the non-stationary traits of speckle noise in OCT imagery. The proposed approach centers on a non-stationary spline-based speckle noise modeling strategy to characterize the speckle noise. The novel method was applied to ultra high-resolution OCT (UHROCT) images of the human retina and corneo-scleral limbus acquired in-vivo that vary in tissue structure and optical properties. Test results showed improved performance of the proposed novel algorithm compared to a number of previously published speckle noise compensation approaches in terms of higher signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR) and better overall visual assessment.

Stochastic speckle noise compensation in optical coherence tomography using non-stationary spline-based speckle noise modelling

PubMed Central

Cameron, Andrew; Lui, Dorothy; Boroomand, Ameneh; Glaister, Jeffrey; Wong, Alexander; Bizheva, Kostadinka

2013-01-01

Optical coherence tomography (OCT) allows for non-invasive 3D visualization of biological tissue at cellular level resolution. Often hindered by speckle noise, the visualization of important biological tissue details in OCT that can aid disease diagnosis can be improved by speckle noise compensation. A challenge with handling speckle noise is its inherent non-stationary nature, where the underlying noise characteristics vary with the spatial location. In this study, an innovative speckle noise compensation method is presented for handling the non-stationary traits of speckle noise in OCT imagery. The proposed approach centers on a non-stationary spline-based speckle noise modeling strategy to characterize the speckle noise. The novel method was applied to ultra high-resolution OCT (UHROCT) images of the human retina and corneo-scleral limbus acquired in-vivo that vary in tissue structure and optical properties. Test results showed improved performance of the proposed novel algorithm compared to a number of previously published speckle noise compensation approaches in terms of higher signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR) and better overall visual assessment. PMID:24049697

Speckle disturbance limit in laser-based cinema projection systems

PubMed Central

Verschaffelt, Guy; Roelandt, Stijn; Meuret, Youri; Van den Broeck, Wendy; Kilpi, Katriina; Lievens, Bram; Jacobs, An; Janssens, Peter; Thienpont, Hugo

2015-01-01

In a multi-disciplinary effort, we investigate the level of speckle that can be tolerated in a laser cinema projector based on a quality of experience experiment with movie clips shown to a test audience in a real-life movie theatre setting. We identify a speckle disturbance threshold by statistically analyzing the observers’ responses for different values of the amount of speckle, which was monitored using a well-defined speckle measurement method. The analysis shows that the speckle perception of a human observer is not only dependent on the objectively measured amount of speckle, but it is also strongly influenced by the image content. The speckle disturbance limit for movies turns out to be substantially larger than that for still images, and hence is easier to attain. PMID:26370531

Speckle disturbance limit in laser-based cinema projection systems.

PubMed

Verschaffelt, Guy; Roelandt, Stijn; Meuret, Youri; Van den Broeck, Wendy; Kilpi, Katriina; Lievens, Bram; Jacobs, An; Janssens, Peter; Thienpont, Hugo

2015-09-15

In a multi-disciplinary effort, we investigate the level of speckle that can be tolerated in a laser cinema projector based on a quality of experience experiment with movie clips shown to a test audience in a real-life movie theatre setting. We identify a speckle disturbance threshold by statistically analyzing the observers' responses for different values of the amount of speckle, which was monitored using a well-defined speckle measurement method. The analysis shows that the speckle perception of a human observer is not only dependent on the objectively measured amount of speckle, but it is also strongly influenced by the image content. The speckle disturbance limit for movies turns out to be substantially larger than that for still images, and hence is easier to attain.

Speckle disturbance limit in laser-based cinema projection systems

NASA Astrophysics Data System (ADS)

Verschaffelt, Guy; Roelandt, Stijn; Meuret, Youri; van den Broeck, Wendy; Kilpi, Katriina; Lievens, Bram; Jacobs, An; Janssens, Peter; Thienpont, Hugo

2015-09-01

In a multi-disciplinary effort, we investigate the level of speckle that can be tolerated in a laser cinema projector based on a quality of experience experiment with movie clips shown to a test audience in a real-life movie theatre setting. We identify a speckle disturbance threshold by statistically analyzing the observers’ responses for different values of the amount of speckle, which was monitored using a well-defined speckle measurement method. The analysis shows that the speckle perception of a human observer is not only dependent on the objectively measured amount of speckle, but it is also strongly influenced by the image content. The speckle disturbance limit for movies turns out to be substantially larger than that for still images, and hence is easier to attain.

Speckle phase near random surfaces

NASA Astrophysics Data System (ADS)

Chen, Xiaoyi; Cheng, Chuanfu; An, Guoqiang; Han, Yujing; Rong, Zhenyu; Zhang, Li; Zhang, Meina

2018-03-01

Based on Kirchhoff approximation theory, the speckle phase near random surfaces with different roughness is numerically simulated. As expected, the properties of the speckle phase near the random surfaces are different from that in far field. In addition, as scattering distances and roughness increase, the average fluctuations of the speckle phase become larger. Unusually, the speckle phase is somewhat similar to the corresponding surface topography. We have performed experiments to verify the theoretical simulation results. Studies in this paper contribute to understanding the evolution of speckle phase near a random surface and provide a possible way to identify a random surface structure based on its speckle phase.

Improving image-quality of interference fringes of out-of-plane vibration using temporal speckle pattern interferometry and standard deviation for piezoelectric plates.

PubMed

Chien-Ching Ma; Ching-Yuan Chang

2013-07-01

Interferometry provides a high degree of accuracy in the measurement of sub-micrometer deformations; however, the noise associated with experimental measurement undermines the integrity of interference fringes. This study proposes the use of standard deviation in the temporal domain to improve the image quality of patterns obtained from temporal speckle pattern interferometry. The proposed method combines the advantages of both mean and subtractive methods to remove background noise and ambient disturbance simultaneously, resulting in high-resolution images of excellent quality. The out-of-plane vibration of a thin piezoelectric plate is the main focus of this study, providing information useful to the development of energy harvesters. First, ten resonant states were measured using the proposed method, and both mode shape and resonant frequency were investigated. We then rebuilt the phase distribution of the first resonant mode based on the clear interference patterns obtained using the proposed method. This revealed instantaneous deformations in the dynamic characteristics of the resonant state. The proposed method also provides a frequency-sweeping function, facilitating its practical application in the precise measurement of resonant frequency. In addition, the mode shapes and resonant frequencies obtained using the proposed method were recorded and compared with results obtained using finite element method and laser Doppler vibrometery, which demonstrated close agreement.

High-speed time-reversed ultrasonically encoded (TRUE) optical focusing inside dynamic scattering media at 793 nm

NASA Astrophysics Data System (ADS)

Liu, Yan; Lai, Puxiang; Ma, Cheng; Xu, Xiao; Suzuki, Yuta; Grabar, Alexander A.; Wang, Lihong V.

2014-03-01

Time-reversed ultrasonically encoded (TRUE) optical focusing is an emerging technique that focuses light deep into scattering media by phase-conjugating ultrasonically encoded diffuse light. In previous work, the speed of TRUE focusing was limited to no faster than 1 Hz by the response time of the photorefractive phase conjugate mirror, or the data acquisition and streaming speed of the digital camera; photorefractive-crystal-based TRUE focusing was also limited to the visible spectral range. These time-consuming schemes prevent this technique from being applied in vivo, since living biological tissue has a speckle decorrelation time on the order of a millisecond. In this work, using a Tedoped Sn2P2S6 photorefractive crystal at a near-infrared wavelength of 793 nm, we achieved TRUE focusing inside dynamic scattering media having a speckle decorrelation time as short as 7.7 ms. As the achieved speed approaches the tissue decorrelation rate, this work is an important step forward toward in vivo applications of TRUE focusing in deep tissue imaging, photodynamic therapy, and optical manipulation.

Speckled lentiginous nevus: A rare presentation associated with motor neuropathy and muscular atrophy in a child.

PubMed

Greywal, Tanya; Matiz, Catalina

2018-05-01

Speckled lentiginous nevus syndrome has been described in individuals with a speckled lentiginous nevus with rare associated neurologic deficits. Because speckled lentiginous nevus syndrome almost always affects adults, it is not typically considered when evaluating children. We present the first reported case of speckled lentiginous nevus syndrome presenting in a young child with muscle atrophy and motor deficits affecting muscles along the same distribution as the speckled lentiginous nevus. © 2018 Wiley Periodicals, Inc.

Integrating single-point vibrometer and full-field electronic speckle pattern interferometer to evaluate a micro-speaker

NASA Astrophysics Data System (ADS)

Chang, Wen-Chi; Chen, Yu-Chi; Chien, Chih-Jen; Wang, An-Bang; Lee, Chih-Kung

2011-04-01

A testing system contains an advanced vibrometer/interferometer device (AVID) and a high-speed electronic speckle pattern interferometer (ESPI) was developed. AVID is a laser Doppler vibrometer that can be used to detect single-point linear and angular velocity with DC to 20 MHz bandwidth and with nanometer resolution. In swept frequency mode, frequency response from mHz to MHz of the structure of interest can be measured. The ESPI experimental setup can be used to measure full-field out-of-plane displacement. A 5-1 phase shifting method and a correlation algorithm were used to analyze the phase difference between the reference signal and the speckle signal scattered from the sample surface. In order to show the efficiency and effectiveness of AVID and ESPI, we designed a micro-speaker composed of a plate with fixed boundaries and two piezo-actuators attached to the sides of the plate. The AVID was used to measure the vibration of one of the piezo-actuators and the ESPI was adopted to measure the two-dimensional out-of-plane displacement of the plate. A microphone was used to measure the acoustic response created by the micro-speaker. Driving signal includes random signal, sinusoidal signal, amplitude modulated high-frequency carrier signal, etc. Angular response induced by amplitude modulated high-frequency carrier signal was found to be significantly narrower than the frequency responses created by other types of driving signals. The validity of our newly developed NDE system are detailed by comparing the relationship between the vibration signal of the micro-speaker and the acoustic field generated.

Inducible fluorescent speckle microscopy

PubMed Central

Aguiar, Paulo; Belsley, Michael; Maiato, Helder

2016-01-01

The understanding of cytoskeleton dynamics has benefited from the capacity to generate fluorescent fiducial marks on cytoskeleton components. Here we show that light-induced imprinting of three-dimensional (3D) fluorescent speckles significantly improves speckle signal and contrast relative to classic (random) fluorescent speckle microscopy. We predict theoretically that speckle imprinting using photobleaching is optimal when the laser energy and fluorophore responsivity are related by the golden ratio. This relation, which we confirm experimentally, translates into a 40% remaining signal after speckle imprinting and provides a rule of thumb in selecting the laser power required to optimally prepare the sample for imaging. This inducible speckle imaging (ISI) technique allows 3D speckle microscopy to be performed in readily available libraries of cell lines or primary tissues expressing fluorescent proteins and does not preclude conventional imaging before speckle imaging. As a proof of concept, we use ISI to measure metaphase spindle microtubule poleward flux in primary cells and explore a scaling relation connecting microtubule flux to metaphase duration. PMID:26783303

Inducible fluorescent speckle microscopy.

PubMed

Pereira, António J; Aguiar, Paulo; Belsley, Michael; Maiato, Helder

2016-01-18

The understanding of cytoskeleton dynamics has benefited from the capacity to generate fluorescent fiducial marks on cytoskeleton components. Here we show that light-induced imprinting of three-dimensional (3D) fluorescent speckles significantly improves speckle signal and contrast relative to classic (random) fluorescent speckle microscopy. We predict theoretically that speckle imprinting using photobleaching is optimal when the laser energy and fluorophore responsivity are related by the golden ratio. This relation, which we confirm experimentally, translates into a 40% remaining signal after speckle imprinting and provides a rule of thumb in selecting the laser power required to optimally prepare the sample for imaging. This inducible speckle imaging (ISI) technique allows 3D speckle microscopy to be performed in readily available libraries of cell lines or primary tissues expressing fluorescent proteins and does not preclude conventional imaging before speckle imaging. As a proof of concept, we use ISI to measure metaphase spindle microtubule poleward flux in primary cells and explore a scaling relation connecting microtubule flux to metaphase duration. © 2016 Pereira et al.

Dispersion of speckle suppression efficiency for binary DOE structures: spectral domain and coherent matrix approaches.

PubMed

Lapchuk, Anatoliy; Prygun, Olexandr; Fu, Minglei; Le, Zichun; Xiong, Qiyuan; Kryuchyn, Andriy

2017-06-26

We present the first general theoretical description of speckle suppression efficiency based on an active diffractive optical element (DOE). The approach is based on spectral analysis of diffracted beams and a coherent matrix. Analytical formulae are obtained for the dispersion of speckle suppression efficiency using different DOE structures and different DOE activation methods. We show that a one-sided 2D DOE structure has smaller speckle suppression range than a two-sided 1D DOE structure. Both DOE structures have sufficient speckle suppression range to suppress low-order speckles in the entire visible range, but only the two-sided 1D DOE can suppress higher-order speckles. We also show that a linear shift 2D DOE in a laser projector with a large numerical aperture has higher effective speckle suppression efficiency than the method using switching or step-wise shift DOE structures. The generalized theoretical models elucidate the mechanism and practical realization of speckle suppression.

Dual beam optical interferometer

NASA Technical Reports Server (NTRS)

Gutierrez, Roman C. (Inventor)

2003-01-01

A dual beam interferometer device is disclosed that enables moving an optics module in a direction, which changes the path lengths of two beams of light. The two beams reflect off a surface of an object and generate different speckle patterns detected by an element, such as a camera. The camera detects a characteristic of the surface.

Electronic speckle-pattern interferometry (ESPI) applied to the study of mechanical behavior of human jaws

NASA Astrophysics Data System (ADS)

Roman, Juan F.; Moreno de las Cuevas, Vincente; Salgueiro, Jose R.; Suarez, David; Fernandez, Paula; Gallas, Mercedes; Blanchard, Alain

1996-01-01

The study of the mechanical behavior of the human jaw during chewing is helpful in several specific medical fields that cover the maxillo-facial area. In this work, electronic speckle pattern interferometry has been applied to study dead jaw bones under external stress which simulates the deformations induced during chewing. Fringes obtained after subtraction of two images of the jaw, the image of the relaxed jaw and that of the jaw under stress, give us information about the most stressed zones. The interferometric analysis proposed here is attractive as it can be done in real time with the jaw under progressive stress. Image processing can be applied for improving the quality of fringes. This research can be of help in orthognathic surgery, for example in diagnosis and treatment of fractured jaws, in oral surgery, and in orthodontics because it would help us to know the stress dispersion when we insert an osseointegrated implant or place an orthodontic appliance, respectively. Studying fragments of human jaw some results about its elasticity and flexibility were obtained.

Fiber-optic security monitoring sensor

NASA Astrophysics Data System (ADS)

Englund, Marja; Ipatti, Ari; Karioja, Pentti

1997-09-01

In security monitoring, fiber-optic sensors are advantageous because strong and rugged optical fibers are thin, light, flexible and immune to electromagnetic interference. Optical fibers packaged into cables, such as, building and underground cables, can be used to detect even slightest disturbances, movements, vibrations, pressure changes and impacts along their entire length. When running an optical cable around a structure, and when using speckle pattern recognition technique for alarm monitoring, the distributed monitoring of the structure is possible. The sensing cable can be strung along fences, buried underground, embedded into concrete, mounted on walls, floors and ceilings, or wrapped around the specific components. In this paper, a fiber-optic security monitoring sensor based on speckle pattern monitoring is described. The description of the measuring method and the results of the experimental fiber installations are given. The applicability of embedded and surface mounted fibers to monitor the pressure and impact induced vibrations of fences and concrete structures as well as the loosening of critical parts in a power plant machinery were demonstrated in field and laboratory conditions. The experiences related to the applications and optical cable types are also discussed.

Fiber optic security monitoring sensor

NASA Astrophysics Data System (ADS)

Englund, Marja; Ipatti, Ari; Karioja, Pentti

1997-09-01

In security monitoring, fiber-optic sensors are advantageous because strong and rugged optical fibers are thin, light, flexible and immune to electromagnetic interference. Optical fibers packaged into cables, such as, building and underground cables, can be used to detect even slightest disturbances, movements, vibrations, pressure changes and impacts along their entire length. When running an optical cable around a structure, and when using speckle pattern recognition technique for alarm monitoring, the distributed monitoring of the structure is possible. The sensing cable can be strung along fences, buried underground, embedded into concrete, mounted on walls, floors and ceilings, or wrapped around the specific components. In this paper, a fiber-optic security monitoring sensor based on speckle pattern monitoring is described. The description of the measuring method and the results of the experimental fiber installations are given. The applicability of embedded and surface mounted fibers to monitor the pressure and impact induced vibrations of fences and concrete structures as well as the loosening of critical parts in a power plant machinery were demonstrated in field and laboratory conditions. The experiences related to the applications and optical cable types are also discussed.

Identification of a chemical inhibitor for nuclear speckle formation: Implications for the function of nuclear speckles in regulation of alternative pre-mRNA splicing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kurogi, Yutaro; Matsuo, Yota; Mihara, Yuki

2014-03-28

Highlights: • We identified tubercidin as a compound inducing aberrant formation of the speckles. • Tubercidin causes delocalization of poly (A){sup +}RNAs from nuclear speckles. • Tubercidin induces dispersion of splicing factors from nuclear speckles. • Tubercidin affects alternative pre-mRNA splicing. • Nuclear speckles play a role in regulation of alternative pre-mRNA splicing. - Abstract: Nuclear speckles are subnuclear structures enriched with RNA processing factors and poly (A){sup +} RNAs comprising mRNAs and poly (A){sup +} non-coding RNAs (ncRNAs). Nuclear speckles are thought to be involved in post-transcriptional regulation of gene expression, such as pre-mRNA splicing. By screening 3585 culturemore » extracts of actinomycetes with in situ hybridization using an oligo dT probe, we identified tubercidin, an analogue of adenosine, as an inhibitor of speckle formation, which induces the delocalization of poly (A){sup +} RNA and dispersion of splicing factor SRSF1/SF2 from nuclear speckles in HeLa cells. Treatment with tubercidin also decreased steady-state MALAT1 long ncRNA, thought to be involved in the retention of SRSF1/SF2 in nuclear speckles. In addition, we found that tubercidin treatment promoted exon skipping in the alternative splicing of Clk1 pre-mRNA. These results suggest that nuclear speckles play a role in modulating the concentration of splicing factors in the nucleoplasm to regulate alternative pre-mRNA splicing.« less

[Laser speckle suppression due to dynamic multiple scattering scheme introduced by oblique incidence].

PubMed

Xu, Mei-fang; Gao, Wen-hong; Shi, Yun-bo; Wang, Hao-quan; Du, Bin-bin

2014-06-01

Speckle suppression has been the research focus in laser display technology. In the present paper, the relation between multiple scattering and the size of speckle grains is established by analyzing the properties of speckle generated by the laser beam through SiO2 suspension. Combined with dynamic light scattering theory, laser speckle suppression due to dynamic multiple scattering scheme introduced by oblique incidence is proposed. A speckle suppression element consists of a static diffuser and a light pipe containing the water suspension of SiO2 microspheres with a diameter of 300 nm and a molar concentration of 3.0 x 10(-4) μm3, which is integrated with the laser display system. The laser beam with different incident angles into the SiO2 suspension affecting the contrast of the speckle images is analyzed by the experiments. The results demonstrate that the contrast of the speckle image can be reduced to 0.067 from 0.43 when the beam with the incident angle of approximately 8 degrees illuminates into the SiO2 suspension. The spatial average of speckle granules and the temporal average of speckle images were achieved by the proposed method, which improved the effect of speckle suppression. The proposed element for speckle suppression improved the reliability and reduced the cost of laser projection system, since no mechanical vibration is needed and it is convenient to integrate the element with the existing projection system.

Statistical spatio-temporal properties of the Laser MegaJoule speckle

DOE Office of Scientific and Technical Information (OSTI.GOV)

Le Cain, A.; Sajer, J. M.; Riazuelo, G.

2012-10-15

This paper investigates a statistical model to describe the spatial and temporal properties of hot spots generated by the superimposition of multiple laser beams. In the context of the Laser MegaJoule design, we introduce the formula for contrasts, trajectories and velocities of the speckle pattern. Single bundle of four beams, two-cones and three-cones configurations are considered. Statistical properties of the speckle in the zone where all the beams overlap are studied with different configurations of polarizations. These properties are shown to be very different from the case of one single bundle of four beams. The configuration of polarization has onlymore » a slight effect in the two-cones or three cones configuration. Indeed, the impact of the double polarization smoothing is reduced in the area in which all the beams overlap, while it is much more significant when they split. Moreover, the size of the hot-spots decreases as the number of laser beams increases, but we show that their velocity decreases. As a matter of fact, the maximal velocity of hot spots is found to be only about 10{sup -5} of the velocity of light and the integrated contrast is about 15% when the beams overlap.« less

Dynamic laser speckle applied to the analysis of maturation process of irradiated fresh fruits

NASA Astrophysics Data System (ADS)

Vincitorio, F. M.; Budini, N.; Freyre, C.; Mulone, C.; Fiorucci, M. P.; López, A. J.; Ramil, A.

2012-10-01

The treatment of fresh fruits with different doses of ionizing radiation has been found effective for delaying ripening and, in this way, to extend shelf life. This preservation method is likely to produce some functional or constitutive changes in the cellular structure of the fruit. In this work, a test of the effectiveness of fruit irradiation with relatively low doses was performed by using dynamic speckle imaging. Bananas from a same lot were chosen, being a first series of them irradiated with different doses of 0.2, 0.4 and 0.6 kGy (Gy = J/kg) and a second series with doses of 0.2, 0.4, 0.6 and 1 kGy. Non irradiated bananas (0 kGy) were considered as the lot reference for contrast. Irradiation was carried out at the Semi-Industrial Cobalt 60 facility of the Ezeiza Atomic Center, with an activity of 6 × 105 Curie and a dose rate of 28.5 Gy/min. The objective of this work is to analyze differences in the maturation process between irradiated and nonirradiated fruits by means of dynamic speckle pattern evaluation.

Dynamic laser speckle analyzed considering inhomogeneities in the biological sample

NASA Astrophysics Data System (ADS)

Braga, Roberto A.; González-Peña, Rolando J.; Viana, Dimitri Campos; Rivera, Fernando Pujaico

2017-04-01

Dynamic laser speckle phenomenon allows a contactless and nondestructive way to monitor biological changes that are quantified by second-order statistics applied in the images in time using a secondary matrix known as time history of the speckle pattern (THSP). To avoid being time consuming, the traditional way to build the THSP restricts the data to a line or column. Our hypothesis is that the spatial restriction of the information could compromise the results, particularly when undesirable and unexpected optical inhomogeneities occur, such as in cell culture media. It tested a spatial random approach to collect the points to form a THSP. Cells in a culture medium and in drying paint, representing homogeneous samples in different levels, were tested, and a comparison with the traditional method was carried out. An alternative random selection based on a Gaussian distribution around a desired position was also presented. The results showed that the traditional protocol presented higher variation than the outcomes using the random method. The higher the inhomogeneity of the activity map, the higher the efficiency of the proposed method using random points. The Gaussian distribution proved to be useful when there was a well-defined area to monitor.

Earth observation taken by the Expedition 30 crewmember

NASA Image and Video Library

2012-02-22

ISS030-E-099324 (22 Feb. 2012) --- City lights of Dubai, United Arab Emirates are featured in this image photographed by an Expedition 30 crew member on the International Space Station. The City of Dubai?the largest metropolitan area within the emirate of Dubai?is a favorite subject of astronaut photography largely due to the unique island developments situated directly offshore in the Persian Gulf. These artificial archipelagos have been built such that their full design is only visible from the vantage point of an airplane ? or an orbiting spacecraft such as the International Space Station. The city presents an eye-catching appearance at night that vividly displays the urban development pattern. In this detailed nighttime image?taken with a long focal length lens and digital camera optimized for fast response and high light sensitivity?several interesting patterns can be observed. The highways and major streets are sharply defined by yellow-orange lighting, while the commercial and residential areas are resolved into a speckle pattern of individual white, blue, and yellow-orange lights. Several large and brilliantly lit areas are large hotel and mall complexes, including the Burj Khalifa Tower; at 828 meters (2,717 feet) height it is the world?s tallest building. The brilliant lighting of the city contrasts sharply with both the dark Persian Gulf to the northwest, and largely undeveloped and unlit areas to the southeast. Likewise, the clusters of lighting in the Palm Jumeira complex at bottom right correspond to the relatively small part of the archipelago that has been developed. Isolated areas of blurred city lights are due to patchy clouds.

Artificial submicron or nanometer speckle fabricating technique and electron microscope speckle photography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu Zhanwei; Xie Huimin; Fang Daining

2007-03-15

In this article, a novel artificial submicro- or nanometer speckle fabricating technique is proposed by taking advantage of submicro or nanometer particles. In the technique, submicron or nanometer particles were adhered to an object surface by using ultrasonic dispersing technique. The particles on the object surface can be regarded as submicro or nanometer speckle by using a scanning electronic microscope at a special magnification. In addition, an electron microscope speckle photography (EMSP) method is developed to measure in-plane submicron or nanometer deformation of the object coated with the artificial submicro or nanometer speckles. The principle of artificial submicro or nanometermore » speckle fabricating technique and the EMSP method are discussed in detail in this article. Some typical applications of this method are offered. The experimental results verified that the artificial submicro or nanometer speckle fabricating technique and EMSP method is feasible.« less

Multimodal ophthalmic imaging using swept source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography

NASA Astrophysics Data System (ADS)

Malone, Joseph D.; El-Haddad, Mohamed T.; Tye, Logan A.; Majeau, Lucas; Godbout, Nicolas; Rollins, Andrew M.; Boudoux, Caroline; Tao, Yuankai K.

2016-03-01

Scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) benefit clinical diagnostic imaging in ophthalmology by enabling in vivo noninvasive en face and volumetric visualization of retinal structures, respectively. Spectrally encoding methods enable confocal imaging through fiber optics and reduces system complexity. Previous applications in ophthalmic imaging include spectrally encoded confocal scanning laser ophthalmoscopy (SECSLO) and a combined SECSLO-OCT system for image guidance, tracking, and registration. However, spectrally encoded imaging suffers from speckle noise because each spectrally encoded channel is effectively monochromatic. Here, we demonstrate in vivo human retinal imaging using a swept source spectrally encoded scanning laser ophthalmoscope and OCT (SSSESLO- OCT) at 1060 nm. SS-SESLO-OCT uses a shared 100 kHz Axsun swept source, shared scanner and imaging optics, and are detected simultaneously on a shared, dual channel high-speed digitizer. SESLO illumination and detection was performed using the single mode core and multimode inner cladding of a double clad fiber coupler, respectively, to preserve lateral resolution while improving collection efficiency and reducing speckle contrast at the expense of confocality. Concurrent en face SESLO and cross-sectional OCT images were acquired with 1376 x 500 pixels at 200 frames-per-second. Our system design is compact and uses a shared light source, imaging optics, and digitizer, which reduces overall system complexity and ensures inherent co-registration between SESLO and OCT FOVs. En face SESLO images acquired concurrent with OCT cross-sections enables lateral motion tracking and three-dimensional volume registration with broad applications in multivolume OCT averaging, image mosaicking, and intraoperative instrument tracking.

Pixel-based speckle adjustment for noise reduction in Fourier-domain OCT images.

PubMed

Zhang, Anqi; Xi, Jiefeng; Sun, Jitao; Li, Xingde

2017-03-01

Speckle resides in OCT signals and inevitably effects OCT image quality. In this work, we present a novel method for speckle noise reduction in Fourier-domain OCT images, which utilizes the phase information of complex OCT data. In this method, speckle area is pre-delineated pixelwise based on a phase-domain processing method and then adjusted by the results of wavelet shrinkage of the original image. Coefficient shrinkage method such as wavelet or contourlet is applied afterwards for further suppressing the speckle noise. Compared with conventional methods without speckle adjustment, the proposed method demonstrates significant improvement of image quality.

DH and ESPI laser interferometry applied to the restoration shrinkage assessment

NASA Astrophysics Data System (ADS)

Campos, L. M. P.; Parra, D. F.; Vasconcelos, M. R.; Vaz, M.; Monteiro, J.

2014-01-01

In dental restoration postoperative marginal leakage is commonly associated to polymerization shrinkage effects. In consequence the longevity and quality of restorative treatment depends on the shrinkage mechanisms of the composite filling during the polymerization. In this work the development of new techniques for evaluation of those effects under light-induced polymerization of dental nano composite fillings is reported. The composite resins activated by visible light, initiate the polymerization process by absorbing light in wavelengths at about 470 nm. The techniques employed in the contraction assessment were digital holography (DH) and Electronic Speckle Pattern Interferometry (ESPI) based on laser interferometry. A satisfactory resolution was achieved in the non-contact displacement field measurements on small objects concerning the experimental dental samples. According to a specific clinical protocol, natural teeth were used (human mandibular premolars). A class I cavity was drilled and restored with nano composite material, according to Black principles. The polymerization was monitored by DH and ESPI in real time during the cure reaction of the restoration. The total displacement reported for the material in relation of the tooth wall was 3.7 μm (natural tooth). The technique showed the entire tooth surface (wall) deforming during polymerization shrinkage.

Diffractive optical element in materials testing

NASA Astrophysics Data System (ADS)

Silvennoinen, Raimo V. J.; Peiponen, Kai-Erik

1998-09-01

The object of this paper is to present a sensor based on diffractive optics that can be applied for the materials testing. The present sensor, which is based on the use of a computer-generated hologram (CGH) exploits the holographic imagery. The CGH-sensor was introduced for inspection of surface roughness and flatness of metal surfaces. The results drawn out by the present sensor are observed to be in accordance with the experimental data. Together with the double exposure holographic interferometry (DEHI) and digital electronic speckle pattern interferometry (DSPI) in elasticity inspection, the sensor was applied for the investigations of surface quality of opaque fragile materials, which are pharmaceutical compacts. The optical surface quality was observed to be related to the porosity of the pharmaceutical tablets. The CGH-sensor was also applied for investigations of optical quality of thin films as PLZT ceramics and coating of pharmaceutical compacts. The surfaces of PLZT samples showed fluctuations in optical curvature, and wedgeness for all the cases studied. For pharmaceutical compacts, the optical signals were observed to depend to a great extent on the optical constants of the coatings and the substrates, and in addition to the surface porosity under the coating.

Effect of incidence/observation angles and angular diversity on speckle reduction by wavelength diversity in laser projection systems.

PubMed

Yamada, Hirotaka; Moriyasu, Kengo; Sato, Hiroto; Hatanaka, Hidekazu

2017-12-11

The speckle reduction for laser projectors has been vigorously studied because speckle causes a serious deterioration in image quality. Most speckle reduction methods can be categorized into wavelength diversity, angular diversity and polarization diversity, which are usually treated independently. In this paper, it is shown that the effect of wavelength diversity and angular diversity on speckle reduction is not independent, and that the effect of wavelength also depends on incidence and observation angles on screen. The speckle reduction effect by wavelength diversity is smaller when the angular diversity is larger. Also, the speckle reduction effect is investigated on various screens including matte and silver screens, and it is shown that the effect of wavelength diversity is larger on matte screen than on silver screen.

Rayleigh-maximum-likelihood bilateral filter for ultrasound image enhancement.

PubMed

Li, Haiyan; Wu, Jun; Miao, Aimin; Yu, Pengfei; Chen, Jianhua; Zhang, Yufeng

2017-04-17

Ultrasound imaging plays an important role in computer diagnosis since it is non-invasive and cost-effective. However, ultrasound images are inevitably contaminated by noise and speckle during acquisition. Noise and speckle directly impact the physician to interpret the images and decrease the accuracy in clinical diagnosis. Denoising method is an important component to enhance the quality of ultrasound images; however, several limitations discourage the results because current denoising methods can remove noise while ignoring the statistical characteristics of speckle and thus undermining the effectiveness of despeckling, or vice versa. In addition, most existing algorithms do not identify noise, speckle or edge before removing noise or speckle, and thus they reduce noise and speckle while blurring edge details. Therefore, it is a challenging issue for the traditional methods to effectively remove noise and speckle in ultrasound images while preserving edge details. To overcome the above-mentioned limitations, a novel method, called Rayleigh-maximum-likelihood switching bilateral filter (RSBF) is proposed to enhance ultrasound images by two steps: noise, speckle and edge detection followed by filtering. Firstly, a sorted quadrant median vector scheme is utilized to calculate the reference median in a filtering window in comparison with the central pixel to classify the target pixel as noise, speckle or noise-free. Subsequently, the noise is removed by a bilateral filter and the speckle is suppressed by a Rayleigh-maximum-likelihood filter while the noise-free pixels are kept unchanged. To quantitatively evaluate the performance of the proposed method, synthetic ultrasound images contaminated by speckle are simulated by using the speckle model that is subjected to Rayleigh distribution. Thereafter, the corrupted synthetic images are generated by the original image multiplied with the Rayleigh distributed speckle of various signal to noise ratio (SNR) levels and added with Gaussian distributed noise. Meanwhile clinical breast ultrasound images are used to visually evaluate the effectiveness of the method. To examine the performance, comparison tests between the proposed RSBF and six state-of-the-art methods for ultrasound speckle removal are performed on simulated ultrasound images with various noise and speckle levels. The results of the proposed RSBF are satisfying since the Gaussian noise and the Rayleigh speckle are greatly suppressed. The proposed method can improve the SNRs of the enhanced images to nearly 15 and 13 dB compared with images corrupted by speckle as well as images contaminated by speckle and noise under various SNR levels, respectively. The RSBF is effective in enhancing edge while smoothing the speckle and noise in clinical ultrasound images. In the comparison experiments, the proposed method demonstrates its superiority in accuracy and robustness for denoising and edge preserving under various levels of noise and speckle in terms of visual quality as well as numeric metrics, such as peak signal to noise ratio, SNR and root mean squared error. The experimental results show that the proposed method is effective for removing the speckle and the background noise in ultrasound images. The main reason is that it performs a "detect and replace" two-step mechanism. The advantages of the proposed RBSF lie in two aspects. Firstly, each central pixel is classified as noise, speckle or noise-free texture according to the absolute difference between the target pixel and the reference median. Subsequently, the Rayleigh-maximum-likelihood filter and the bilateral filter are switched to eliminate speckle and noise, respectively, while the noise-free pixels are unaltered. Therefore, it is implemented with better accuracy and robustness than the traditional methods. Generally, these traits declare that the proposed RSBF would have significant clinical application.

Imaging of optically diffusive media by use of opto-elastography

NASA Astrophysics Data System (ADS)

Bossy, Emmanuel; Funke, Arik R.; Daoudi, Khalid; Tanter, Mickael; Fink, Mathias; Boccara, Claude

2007-02-01

We present a camera-based optical detection scheme designed to detect the transient motion created by the acoustic radiation force in elastic media. An optically diffusive tissue mimicking phantom was illuminated with coherent laser light, and a high speed camera (2 kHz frame rate) was used to acquire and cross-correlate consecutive speckle patterns. Time-resolved transient decorrelations of the optical speckle were measured as the results of localised motion induced in the medium by the radiation force and subsequent propagating shear waves. As opposed to classical acousto-optic techniques which are sensitive to vibrations induced by compressional waves at ultrasonic frequencies, the proposed technique is sensitive only to the low frequency transient motion induced in the medium by the radiation force. It therefore provides a way to assess both optical and shear mechanical properties.

Pixel-based speckle adjustment for noise reduction in Fourier-domain OCT images

PubMed Central

Zhang, Anqi; Xi, Jiefeng; Sun, Jitao; Li, Xingde

2017-01-01

Speckle resides in OCT signals and inevitably effects OCT image quality. In this work, we present a novel method for speckle noise reduction in Fourier-domain OCT images, which utilizes the phase information of complex OCT data. In this method, speckle area is pre-delineated pixelwise based on a phase-domain processing method and then adjusted by the results of wavelet shrinkage of the original image. Coefficient shrinkage method such as wavelet or contourlet is applied afterwards for further suppressing the speckle noise. Compared with conventional methods without speckle adjustment, the proposed method demonstrates significant improvement of image quality. PMID:28663860

Correlation of Spatially Filtered Dynamic Speckles in Distance Measurement Application

DOE Office of Scientific and Technical Information (OSTI.GOV)

Semenov, Dmitry V.; Nippolainen, Ervin; Kamshilin, Alexei A.

2008-04-15

In this paper statistical properties of spatially filtered dynamic speckles are considered. This phenomenon was not sufficiently studied yet while spatial filtering is an important instrument for speckles velocity measurements. In case of spatial filtering speckle velocity information is derived from the modulation frequency of filtered light power which is measured by photodetector. Typical photodetector output is represented by a narrow-band random noise signal which includes non-informative intervals. Therefore more or less precious frequency measurement requires averaging. In its turn averaging implies uncorrelated samples. However, conducting research we found that correlation is typical property not only of dynamic speckle patternsmore » but also of spatially filtered speckles. Using spatial filtering the correlation is observed as a response of measurements provided to the same part of the object surface or in case of simultaneously using several adjacent photodetectors. Found correlations can not be explained using just properties of unfiltered dynamic speckles. As we demonstrate the subject of this paper is important not only from pure theoretical point but also from the point of applied speckle metrology. E.g. using single spatial filter and an array of photodetector can greatly improve accuracy of speckle velocity measurements.« less

Speckle-learning-based object recognition through scattering media.

PubMed

Ando, Takamasa; Horisaki, Ryoichi; Tanida, Jun

2015-12-28

We experimentally demonstrated object recognition through scattering media based on direct machine learning of a number of speckle intensity images. In the experiments, speckle intensity images of amplitude or phase objects on a spatial light modulator between scattering plates were captured by a camera. We used the support vector machine for binary classification of the captured speckle intensity images of face and non-face data. The experimental results showed that speckles are sufficient for machine learning.

Speckle reduction in laser projection displays through angle and wavelength diversity.

PubMed

Tran, Trinh-Thi-Kim; Svensen, Øyvind; Chen, Xuyuan; Akram, Muhammad Nadeem

2016-02-20

Speckle is the main obstacle for the use of laser light sources in projection technology. This paper focuses on speckle suppression by the reduction of temporal coherence which is provided by the broadband laser light. The investigation of the effect of laser spectrum width and multiple lasers on speckle contrast is discussed. A broader spectrum width of the laser light is attained by the use of multiple semiconductor laser diodes of the broad area type. Measurements of speckle contrast with and without angle diversity are performed for two and four laser diodes. The measurement of speckle contrast for a single laser diode is also presented for comparison. The experimental results show that multiple laser diodes provide lower speckle contrast as compared to a single laser diode. In addition, it is also shown in this paper that the wavelength distribution of independent laser diodes has an effect on speckle contrast. Two different types of blue laser diodes, Nichia NUB802T and Nichia NUB801E, which have slightly different central wavelengths, were used for the measurements. Four laser diodes with a combination of two types of laser diodes offer better speckle contrast reduction than four laser diodes of the same type due to an effective broader spectrum. Additional speckle contrast reduction is achieved through the angle diversity by using a dynamic deformable mirror.

Speckle Noise in Highly Corrected Coronagraphs

NASA Technical Reports Server (NTRS)

Bloemhof, Eric E.

2004-01-01

Speckles in a highly corrected adaptive optic imaging system have been studied through numerical simulations and through analytic and algebraic investigations of the Fourier-optical expressions connecting pupil plane and focal plane, which simplify at high Strehl ratio. Significant insights into the behavior of speckles, and the speckle noise caused when they vary over time, have thus been gained. Such speckle noise is expected to set key limits on the sensitivity of searches for companions around other stars, including extrasolar planets. In most cases, it is advantageous to use a coronagraph of some kind to suppress the bright primary star and so enhance the dynamic range of companion searches. In the current paper, I investigate speckle behavior and its impact on speckle noise in some common coronagraphic architectures, including the classical Lyot coronagraph and the new four quadrant phase mask (FQPM) concept.

Real-time speckle reduction in optical coherence tomography using the dual window method.

PubMed

Zhao, Yang; Chu, Kengyeh K; Eldridge, Will J; Jelly, Evan T; Crose, Michael; Wax, Adam

2018-02-01

Speckle is an intrinsic noise of interferometric signals which reduces contrast and degrades the quality of optical coherence tomography (OCT) images. Here, we present a frequency compounding speckle reduction technique using the dual window (DW) method. Using the DW method, speckle noise is reduced without the need to acquire multiple frames. A ~25% improvement in the contrast-to-noise ratio (CNR) was achieved using the DW speckle reduction method with only minimal loss (~17%) in axial resolution. We also demonstrate that real-time speckle reduction can be achieved at a B-scan rate of ~21 frames per second using a graphic processing unit (GPU). The DW speckle reduction technique can work on any existing OCT instrument without further system modification or extra components. This makes it applicable both in real-time imaging systems and during post-processing.

Spatial filtering velocimetry of objective speckles for measuring out-of-plane motion.

PubMed

Jakobsen, M L; Yura, H T; Hanson, S G

2012-03-20

This paper analyzes the dynamics of objective laser speckles as the distance between the object and the observation plane continuously changes. With the purpose of applying optical spatial filtering velocimetry to the speckle dynamics, in order to measure out-of-plane motion in real time, a rotational symmetric spatial filter is designed. The spatial filter converts the speckle dynamics into a photocurrent with a quasi-sinusoidal response to the out-of-plane motion. The spatial filter is here emulated with a CCD camera, and is tested on speckles arising from a real application. The analysis discusses the selectivity of the spatial filter, the nonlinear response between speckle motion and observation distance, and the influence of the distance-dependent speckle size. Experiments with the emulated filters illustrate performance and potential applications of the technology. © 2012 Optical Society of America

Coherent X-ray diffraction from collagenous soft tissues.

PubMed

Berenguer de la Cuesta, Felisa; Wenger, Marco P E; Bean, Richard J; Bozec, Laurent; Horton, Michael A; Robinson, Ian K

2009-09-08

Coherent X-ray diffraction has been applied in the imaging of inorganic materials with great success. However, its application to biological specimens has been limited to some notable exceptions, due to the induced radiation damage and the extended nature of biological samples, the last limiting the application of most part of the phasing algorithms. X-ray ptychography, still under development, is a good candidate to overcome such difficulties and become a powerful imaging method for biology. We describe herein the feasibility of applying ptychography to the imaging of biological specimens, in particular collagen rich samples. We report here speckles in diffraction patterns from soft animal tissue, obtained with an optimized small angle X-ray setup that exploits the natural coherence of the beam. By phasing these patterns, dark field images of collagen within tendon, skin, bone, or cornea will eventually be obtained with a resolution of 60-70 nm. We present simulations of the contrast mechanism in collagen based on atomic force microscope images of the samples. Simulations confirmed the 'speckled' nature of the obtained diffraction patterns. Once inverted, the patterns will show the disposition and orientation of the fibers within the tissue, by enhancing the phase contrast between protein and no protein regions of the sample. Our work affords the application of the most innovative coherent X-ray diffraction tools to the study of biological specimens, and this approach will have a significant impact in biology and medicine because it overcomes many of the limits of current microscopy techniques.

Soft x-ray speckle from rough surfaces

NASA Astrophysics Data System (ADS)

Porter, Matthew Stanton

Dynamic light scattering has been of great use in determining diffusion times for polymer solutions. At the same time, polymer thin films are becoming of increasing importance, especially in the semiconductor industry where they are used as photoresists and interlevel dielectrics. As the dimensions of these devices decrease we will reach a point where lasers will no longer be able to probe the length scales of interest. Current laser wavelengths limit the size of observable diffusion lengths to 180-700 nm. This dissertation will discuss attempts at pushing dynamic fight scattering experiments into the soft x-ray region so that we can examine fluctuations in polymer thin films on the molecular length scale. The dissertation explores the possibility of carrying out a dynamic light scattering experiment in the soft x-ray regime. A detailed account of how to meet the basic requirements for a coherent scattering experiment in the soft x-ray regime win be given. In addition, a complete description of the chamber design will be discussed. We used our custom designed scattering chamber to collect reproducible coherent soft x-ray scattering data from etched silicon wafers and from polystyrene coated silicon wafers. The data from the silicon wafers followed the statistics for a well-developed speckle pattern while the data from the polystyrene films exhibited Poisson statistics. We used the data from both the etched wafers and the polystyrene coated wafers to place a lower limit of ~20 Å on the RMS surface roughness of samples which will produce well defined speckle patterns for the current detector setup. Future experiments which use the criteria set forth in this dissertation have the opportunity to be even more successful than this dissertation project.

Method and system for enabling real-time speckle processing using hardware platforms

NASA Technical Reports Server (NTRS)

Ortiz, Fernando E. (Inventor); Kelmelis, Eric (Inventor); Durbano, James P. (Inventor); Curt, Peterson F. (Inventor)

2012-01-01

An accelerator for the speckle atmospheric compensation algorithm may enable real-time speckle processing of video feeds that may enable the speckle algorithm to be applied in numerous real-time applications. The accelerator may be implemented in various forms, including hardware, software, and/or machine-readable media.

Crop identification of SAR data using digital textural analysis

NASA Technical Reports Server (NTRS)

Nuesch, D. R.

1983-01-01

After preprocessing SEASAT SAR data which included slant to ground range transformation, registration to LANDSAT MSS data and appropriate filtering of the raw SAR data to minimize coherent speckle, textural features were developed based upon the spatial gray level dependence method (SGLDM) to compute entropy and inertia as textural measures. It is indicated that the consideration of texture features are very important in SAR data analysis. The SEASAT SAR data are useful for the improvement of field boundary definitions and for an earlier season estimate of corn and soybean area location than is supported by LANDSAT alone.

Real-time speckle reduction in optical coherence tomography using the dual window method

PubMed Central

Zhao, Yang; Chu, Kengyeh K.; Eldridge, Will J.; Jelly, Evan T.; Crose, Michael; Wax, Adam

2018-01-01

Speckle is an intrinsic noise of interferometric signals which reduces contrast and degrades the quality of optical coherence tomography (OCT) images. Here, we present a frequency compounding speckle reduction technique using the dual window (DW) method. Using the DW method, speckle noise is reduced without the need to acquire multiple frames. A ~25% improvement in the contrast-to-noise ratio (CNR) was achieved using the DW speckle reduction method with only minimal loss (~17%) in axial resolution. We also demonstrate that real-time speckle reduction can be achieved at a B-scan rate of ~21 frames per second using a graphic processing unit (GPU). The DW speckle reduction technique can work on any existing OCT instrument without further system modification or extra components. This makes it applicable both in real-time imaging systems and during post-processing. PMID:29552398

Speckle reduction in optical coherence tomography by adaptive total variation method

NASA Astrophysics Data System (ADS)

Wu, Tong; Shi, Yaoyao; Liu, Youwen; He, Chongjun

2015-12-01

An adaptive total variation method based on the combination of speckle statistics and total variation restoration is proposed and developed for reducing speckle noise in optical coherence tomography (OCT) images. The statistical distribution of the speckle noise in OCT image is investigated and measured. With the measured parameters such as the mean value and variance of the speckle noise, the OCT image is restored by the adaptive total variation restoration method. The adaptive total variation restoration algorithm was applied to the OCT images of a volunteer's hand skin, which showed effective speckle noise reduction and image quality improvement. For image quality comparison, the commonly used median filtering method was also applied to the same images to reduce the speckle noise. The measured results demonstrate the superior performance of the adaptive total variation restoration method in terms of image signal-to-noise ratio, equivalent number of looks, contrast-to-noise ratio, and mean square error.

Experimental validation of 2D uncertainty quantification for digital image correlation.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reu, Phillip L.

Because digital image correlation (DIC) has become such an important and standard tool in the toolbox of experimental mechanicists, a complete uncertainty quantification of the method is needed. It should be remembered that each DIC setup and series of images will have a unique uncertainty based on the calibration quality and the image and speckle quality of the analyzed images. Any pretest work done with a calibrated DIC stereo-rig to quantify the errors using known shapes and translations, while useful, do not necessarily reveal the uncertainty of a later test. This is particularly true with high-speed applications where actual testmore » images are often less than ideal. Work has previously been completed on the mathematical underpinnings of DIC uncertainty quantification and is already published, this paper will present corresponding experimental work used to check the validity of the uncertainty equations.« less

Measuring optical phase digitally in coherent metrology systems

NASA Astrophysics Data System (ADS)

Kelly, Damien P.; Ryle, James; Zhao, Liang; Sheridan, John T.

2017-05-01

The accurate measurement of optical phase has many applications in metrology. For biological samples, which appear transparent, the phase data provides information about the refractive index of the sample. In speckle metrology, the phase can be used to estimate stress and strains of a rough surface with high sensitivity. In this theoretical manuscript we compare and contrast the properties of two techniques for estimating the phase distribution of a wave field under the paraxial approximation: (I) A digital holographic system, and (II) An idealized phase retrieval system. Both systems use a CCD or CMOS array to measure the intensities of the wave fields that are reflected from or transmitted through the sample of interest. This introduces a numerical aspect to the problem. For the two systems above we examine how numerical calculations can limit the performance of these systems leading to a near-infinite number of possible solutions.

Measurement of installation deformation of the acetabulum during prosthetic replacement of a hip joint using digital image correlation

NASA Astrophysics Data System (ADS)

Lei, Dong; Bai, Pengxiang; Zhu, Feipeng

2018-01-01

Nowadays, acetabulum prosthesis replacement is widely used in clinical medicine. However, there is no efficient way to evaluate the implantation effect of the prosthesis. Based on a modern photomechanics technique called digital image correlation (DIC), the evaluation method of the installation effect of the acetabulum was established during a prosthetic replacement of a hip joint. The DIC method determines strain field by comparing the speckle images between the undeformed sample and the deformed counterpart. Three groups of experiments were carried out to verify the feasibility of the DIC method on the acetabulum installation deformation test. Experimental results indicate that the installation deformation of acetabulum generally includes elastic deformation (corresponding to the principal strain of about 1.2%) and plastic deformation. When the installation angle is ideal, the plastic deformation can be effectively reduced, which could prolong the service life of acetabulum prostheses.

Laser Speckle Imaging to Monitor Microvascular Blood Flow: A Review.

PubMed

Vaz, Pedro G; Humeau-Heurtier, Anne; Figueiras, Edite; Correia, Carlos; Cardoso, Joao

2016-01-01

Laser speckle is a complex interference phenomenon that can easily be understood, in concept, but is difficult to predict mathematically, because it is a stochastic process. The use of laser speckle to produce images, which can carry many types of information, is called laser speckle imaging (LSI). The biomedical applications of LSI started in 1981 and, since then, many scientists have improved the laser speckle theory and developed different imaging techniques. During this process, some inconsistencies have been propagated up to now. These inconsistencies should be clarified in order to avoid errors in future works. This review presents a review of the laser speckle theory used in biomedical applications. Moreover, we also make a review of the practical concepts that are useful in the construction of laser speckle imagers. This study is not only an exposition of the concepts that can be found in the literature but also a critical analysis of the investigations presented so far. Concepts like scatterers velocity distribution, effect of static scatterers, optimal speckle size, light penetration angle, and contrast computation algorithms are discussed in detail.

Morphological and wavelet features towards sonographic thyroid nodules evaluation.

PubMed

Tsantis, Stavros; Dimitropoulos, Nikos; Cavouras, Dionisis; Nikiforidis, George

2009-03-01

This paper presents a computer-based classification scheme that utilized various morphological and novel wavelet-based features towards malignancy risk evaluation of thyroid nodules in ultrasonography. The study comprised 85 ultrasound images-patients that were cytological confirmed (54 low-risk and 31 high-risk). A set of 20 features (12 based on nodules boundary shape and 8 based on wavelet local maxima located within each nodule) has been generated. Two powerful pattern recognition algorithms (support vector machines and probabilistic neural networks) have been designed and developed in order to quantify the power of differentiation of the introduced features. A comparative study has also been held, in order to estimate the impact speckle had onto the classification procedure. The diagnostic sensitivity and specificity of both classifiers was made by means of receiver operating characteristics (ROC) analysis. In the speckle-free feature set, the area under the ROC curve was 0.96 for the support vector machines classifier whereas for the probabilistic neural networks was 0.91. In the feature set with speckle, the corresponding areas under the ROC curves were 0.88 and 0.86 respectively for the two classifiers. The proposed features can increase the classification accuracy and decrease the rate of missing and misdiagnosis in thyroid cancer control.

Digital micromirror device-based laser-illumination Fourier ptychographic microscopy

PubMed Central

Kuang, Cuifang; Ma, Ye; Zhou, Renjie; Lee, Justin; Barbastathis, George; Dasari, Ramachandra R.; Yaqoob, Zahid; So, Peter T. C.

2015-01-01

We report a novel approach to Fourier ptychographic microscopy (FPM) by using a digital micromirror device (DMD) and a coherent laser source (532 nm) for generating spatially modulated sample illumination. Previously demonstrated FPM systems are all based on partially-coherent illumination, which offers limited throughput due to insufficient brightness. Our FPM employs a high power coherent laser source to enable shot-noise limited high-speed imaging. For the first time, a digital micromirror device (DMD), imaged onto the back focal plane of the illumination objective, is used to generate spatially modulated sample illumination field for ptychography. By coding the on/off states of the micromirrors, the illumination plane wave angle can be varied at speeds more than 4 kHz. A set of intensity images, resulting from different oblique illuminations, are used to numerically reconstruct one high-resolution image without obvious laser speckle. Experiments were conducted using a USAF resolution target and a fiber sample, demonstrating high-resolution imaging capability of our system. We envision that our approach, if combined with a coded-aperture compressive-sensing algorithm, will further improve the imaging speed in DMD-based FPM systems. PMID:26480361

Digital micromirror device-based laser-illumination Fourier ptychographic microscopy.

PubMed

Kuang, Cuifang; Ma, Ye; Zhou, Renjie; Lee, Justin; Barbastathis, George; Dasari, Ramachandra R; Yaqoob, Zahid; So, Peter T C

2015-10-19

We report a novel approach to Fourier ptychographic microscopy (FPM) by using a digital micromirror device (DMD) and a coherent laser source (532 nm) for generating spatially modulated sample illumination. Previously demonstrated FPM systems are all based on partially-coherent illumination, which offers limited throughput due to insufficient brightness. Our FPM employs a high power coherent laser source to enable shot-noise limited high-speed imaging. For the first time, a digital micromirror device (DMD), imaged onto the back focal plane of the illumination objective, is used to generate spatially modulated sample illumination field for ptychography. By coding the on/off states of the micromirrors, the illumination plane wave angle can be varied at speeds more than 4 kHz. A set of intensity images, resulting from different oblique illuminations, are used to numerically reconstruct one high-resolution image without obvious laser speckle. Experiments were conducted using a USAF resolution target and a fiber sample, demonstrating high-resolution imaging capability of our system. We envision that our approach, if combined with a coded-aperture compressive-sensing algorithm, will further improve the imaging speed in DMD-based FPM systems.

Real-time embedded atmospheric compensation for long-range imaging using the average bispectrum speckle method

NASA Astrophysics Data System (ADS)

Curt, Petersen F.; Bodnar, Michael R.; Ortiz, Fernando E.; Carrano, Carmen J.; Kelmelis, Eric J.

2009-02-01

While imaging over long distances is critical to a number of security and defense applications, such as homeland security and launch tracking, current optical systems are limited in resolving power. This is largely a result of the turbulent atmosphere in the path between the region under observation and the imaging system, which can severely degrade captured imagery. There are a variety of post-processing techniques capable of recovering this obscured image information; however, the computational complexity of such approaches has prohibited real-time deployment and hampers the usability of these technologies in many scenarios. To overcome this limitation, we have designed and manufactured an embedded image processing system based on commodity hardware which can compensate for these atmospheric disturbances in real-time. Our system consists of a reformulation of the average bispectrum speckle method coupled with a high-end FPGA processing board, and employs modular I/O capable of interfacing with most common digital and analog video transport methods (composite, component, VGA, DVI, SDI, HD-SDI, etc.). By leveraging the custom, reconfigurable nature of the FPGA, we have achieved performance twenty times faster than a modern desktop PC, in a form-factor that is compact, low-power, and field-deployable.

Preliminary results of real-time in-vitro electronic speckle pattern interferometry (ESPI) measurements in otolaryngology

NASA Astrophysics Data System (ADS)

Conerty, Michelle D.; Castracane, James; Cacace, Anthony T.; Parnes, Steven M.; Gardner, Glendon M.; Miller, Mitchell B.

1995-05-01

Electronic Speckle Pattern Interferometry (ESPI) is a nondestructive optical evaluation technique that is capable of determining surface and subsurface integrity through the quantitative evaluation of static or vibratory motion. By utilizing state of the art developments in the areas of lasers, fiber optics and solid state detector technology, this technique has become applicable in medical research and diagnostics. Based on initial support from NIDCD and continued support from InterScience, Inc., we have been developing a range of instruments for improved diagnostic evaluation in otolaryngological applications based on the technique of ESPI. These compact fiber optic instruments are capable of making real time interferometric measurements of the target tissue. Ongoing development of image post- processing software is currently capable of extracting the desired quantitative results from the acquired interferometric images. The goal of the research is to develop a fully automated system in which the image processing and quantification will be performed in hardware in near real-time. Subsurface details of both the tympanic membrane and vocal cord dynamics could speed the diagnosis of otosclerosis, laryngeal tumors, and aid in the evaluation of surgical procedures.

Wave Propagation and Localization via Quasi-Normal Modes and Transmission Eigenchannels

NASA Astrophysics Data System (ADS)

Wang, Jing; Shi, Zhou; Davy, Matthieu; Genack, Azriel Z.

2013-10-01

Field transmission coefficients for microwave radiation between arrays of points on the incident and output surfaces of random samples are analyzed to yield the underlying quasi-normal modes and transmission eigenchannels of each realization of the sample. The linewidths, central frequencies, and transmitted speckle patterns associated with each of the modes of the medium are found. Modal speckle patterns are found to be strongly correlated leading to destructive interference between modes. This explains distinctive features of transmission spectra and pulsed transmission. An alternate description of wave transport is obtained from the eigenchannels and eigenvalues of the transmission matrix. The maximum transmission eigenvalue, τ1 is near unity for diffusive waves even in turbid samples. For localized waves, τ1 is nearly equal to the dimensionless conductance, which is the sum of all transmission eigenvalues, g = Στn. The spacings between the ensemble averages of successive values of lnτn are constant and equal to the inverse of the bare conductance in accord with predictions by Dorokhov. The effective number of transmission eigenvalues Neff determines the contrast between the peak and background of radiation focused for maximum peak intensity. The connection between the mode and channel approaches is discussed.

Wave Propagation and Localization via Quasi-Normal Modes and Transmission Eigenchannels

NASA Astrophysics Data System (ADS)

Wang, Jing; Shi, Zhou; Davy, Matthieu; Genack, Azriel Z.

Field transmission coefficients for microwave radiation between arrays of points on the incident and output surfaces of random samples are analyzed to yield the underlying quasi-normal modes and transmission eigenchannels of each realization of the sample. The linewidths, central frequencies, and transmitted speckle patterns associated with each of the modes of the medium are found. Modal speckle patterns are found to be strongly correlated leading to destructive interference between modes. This explains distinctive features of transmission spectra and pulsed transmission. An alternate description of wave transport is obtained from the eigenchannels and eigenvalues of the transmission matrix. The maximum transmission eigenvalue, τ1 is near unity for diffusive waves even in turbid samples. For localized waves, τ1 is nearly equal to the dimensionless conductance, which is the sum of all transmission eigenvalues, g = Στn. The spacings between the ensemble averages of successive values of lnτn are constant and equal to the inverse of the bare conductance in accord with predictions by Dorokhov. The effective number of transmission eigenvalues Neff determines the contrast between the peak and background of radiation focused for maximum peak intensity. The connection between the mode and channel approaches is discussed.

SAR Speckle Noise Reduction Using Wiener Filter

NASA Technical Reports Server (NTRS)

Joo, T. H.; Held, D. N.

1983-01-01

Synthetic aperture radar (SAR) images are degraded by speckle. A multiplicative speckle noise model for SAR images is presented. Using this model, a Wiener filter is derived by minimizing the mean-squared error using the known speckle statistics. Implementation of the Wiener filter is discussed and experimental results are presented. Finally, possible improvements to this method are explored.

Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

PubMed Central

Nadort, Annemarie; Woolthuis, Rutger G.; van Leeuwen, Ton G.; Faber, Dirk J.

2013-01-01

We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources. PMID:24298399

Unique Abnormalities in Right Ventricular Longitudinal Strain in Systemic Sclerosis Patients.

PubMed

Mukherjee, Monica; Chung, Shang-En; Ton, Von Khue; Tedford, Ryan J; Hummers, Laura K; Wigley, Fredrick M; Abraham, Theodore P; Shah, Ami A

2016-06-01

Cardiac involvement in systemic sclerosis (scleroderma [SSc]) adversely affects long-term prognosis, often remaining undetectable despite close clinical examination and 2-dimensional echocardiographic monitoring. Speckle-derived strain of the right ventricle (RV) was utilized to detect occult abnormalities in regional and global contractility in SSc patients. A total of 138 SSc patients with technically adequate echocardiograms was studied and compared with 40 age- and sex-matched healthy non-SSc controls. Standard assessment of RV chamber function included tricuspid annular plane systolic excursion and fractional area change. RV longitudinal systolic speckle-derived strain was assessed in the basal, mid, and apical free wall. Tricuspid annular plane systolic excursion was not different between groups (P=0.307). Although fractional area change was lower in SSc patients than in controls (mean, 48.9 versus 55; P=0.002), the mean fractional area change was still within the normal range (>35). In contrast, RV longitudinal systolic speckle-derived strain measures were significantly different between groups, both globally (-20.4% versus -17.7%; P=0.005) and regionally: they were decreased in the apex (-8.5% versus -17.1%; P<0.0001) and mid segments (-12.4% versus -20.9%; P<0.0001), and increased in the base (-32.2% versus -23.3%; P=0.0001) for the SSc group. The regional difference in the base compared with the apex was significantly greater for SSc than for controls (P<0.0001 for interaction). The differences observed in regional strain between SSc and control were unchanged after adjusting for RV systolic pressure. Speckle-derived strain reveals a heterogenous pattern of regional heart strain in SSc that is not detected by conventional measures of function, suggestive of occult RV myocardial disease. © 2016 American Heart Association, Inc.

Calibration of echocardiographic tissue doppler velocity, using simple universally applicable methods

NASA Astrophysics Data System (ADS)

Dhutia, Niti M.; Zolgharni, Massoud; Willson, Keith; Cole, Graham; Nowbar, Alexandra N.; Manisty, Charlotte H.; Francis, Darrel P.

2014-03-01

Some of the challenges with tissue Doppler measurement include: apparent inconsistency between manufacturers, uncertainty over which part of the trace to make measurements and a lack of calibration of measurements. We develop and test tools to solve these problems in echocardiography laboratories. We designed and constructed an actuator and phantom setup to produce automatic reproducible motion, and used it to compare velocities measured using 3 echocardiographic modalities: M-mode, speckle tracking, and tissue Doppler, against a non-ultrasound, optical gold standard. In the clinical phase, 25 patients underwent M-mode, speckle tracking and tissue Doppler measurements of tissue velocities. In-vitro, the M-mode and speckle tracking velocities were concordant with optical assessment. Of the three possible tissue Doppler measurement conventions (outer, middle and inner line) only the middle line agreed with the optical assessment (discrepancy -0.20 (95% confidence interval -0.44 to 0.03)cm/s, p=0.11, outer +5.19(4.65 to 5.73)cm/s, p<0.0001, inner -6.26(-6.87 to -5.65)cm/s, p<0.0001). All 4 studied manufacturers showed a similar pattern. M-mode was therefore chosen as the in-vivo gold standard. Clinical measurements of tissue velocities by speckle tracking and the middle line of the tissue Doppler were concordant with M-mode, while the outer line significantly overestimated (+1.27(0.96 to 1.59)cm/s, p<0.0001) and the inner line underestimated (-1.81(-2.11 to -1.52)cm/s, p<0.0001). Echocardiographic velocity measurements can be calibrated by simple, inexpensive tools. We found that the middle of the tissue Doppler trace represents velocity correctly. Echocardiographers requiring velocities to match between different equipment, settings or modalities should use the middle line as the "guideline".

Tracking quasi-stationary flow of weak fluorescent signals by adaptive multi-frame correlation.

PubMed

Ji, L; Danuser, G

2005-12-01

We have developed a novel cross-correlation technique to probe quasi-stationary flow of fluorescent signals in live cells at a spatial resolution that is close to single particle tracking. By correlating image blocks between pairs of consecutive frames and integrating their correlation scores over multiple frame pairs, uncertainty in identifying a globally significant maximum in the correlation score function has been greatly reduced as compared with conventional correlation-based tracking using the signal of only two consecutive frames. This approach proves robust and very effective in analysing images with a weak, noise-perturbed signal contrast where texture characteristics cannot be matched between only a pair of frames. It can also be applied to images that lack prominent features that could be utilized for particle tracking or feature-based template matching. Furthermore, owing to the integration of correlation scores over multiple frames, the method can handle signals with substantial frame-to-frame intensity variation where conventional correlation-based tracking fails. We tested the performance of the method by tracking polymer flow in actin and microtubule cytoskeleton structures labelled at various fluorophore densities providing imagery with a broad range of signal modulation and noise. In applications to fluorescent speckle microscopy (FSM), where the fluorophore density is sufficiently low to reveal patterns of discrete fluorescent marks referred to as speckles, we combined the multi-frame correlation approach proposed above with particle tracking. This hybrid approach allowed us to follow single speckles robustly in areas of high speckle density and fast flow, where previously published FSM analysis methods were unsuccessful. Thus, we can now probe cytoskeleton polymer dynamics in living cells at an entirely new level of complexity and with unprecedented detail.

Focusing light into desired patterns through turbid media by feedback-based wavefront shaping

NASA Astrophysics Data System (ADS)

Wan, Lipeng; Chen, Ziyang; Huang, Huiling; Pu, Jixiong

2016-07-01

We demonstrate that the focusing of light into desired patterns through turbid media can be realized using feedback-based wavefront shaping. Three desired focused patterns—a triangle, a circle, and a rectangle—are used as examples to study this ability. During the process of modulating scattered light, the Pearson's correlation coefficient is introduced as a feedback signal. It is found that the speckle field formed by the turbid media gradually transforms into the desired pattern through a process of modulation of the input beam wave front. The proposed approach has potential applications in biomedical treatment and laser material processing.

Non-contact optical sensor for detection of glucose concentration using a magneto-optic effect

NASA Astrophysics Data System (ADS)

Ozana, Nisan; Beiderman, Yevgeny; Anand, Arun; Javidi, Baharam; Polani, Sagi; Schwarz, Ariel; Shemer, Amir; García, Javier; Zalevsky, Zeev

2016-03-01

In this paper we aim to experimentally verify a speckle based technique for non-contact measurement of glucose concentration in blood stream while the vision for the final device aims to contain a single wristwatch-style device containing an AC (alternating) electro-magnet generated by a solenoid, a laser and a camera. The experiments presented in work are performed in-vitro in order to verify the effects that are responsible for the operation principle. When a glucose substance is inserted into a solenoid generating an alternating magnetic field it exhibits Faraday rotation which affects the temporal changes of the secondary speckle patterns distribution. The temporal frequency resulting from the AC magnetic field was found to have a lock-in amplification role which increased the observability of the relatively small magneto-optic effect. Experimental results to support the proposed concept are presented.

The development of laser speckle or particle image displacement velocimetry. Part 1: The role of photographic parameters

NASA Technical Reports Server (NTRS)

Lourenco, L. M. M.; Krothapalli, A.

1987-01-01

One of the difficult problems in experimental fluid dynamics remains the determination of the vorticity field in fluid flows. Recently, a novel velocity measurement technique, commonly known as Laser Speckle or Particle Image Displacement Velocimetry became available. This technique permits the simultaneous visualization of the 2 dimensional streamline pattern in unsteady flows and the quantification of the velocity field. The main advantage of this new technique is that the whole 2 dimensional velocity field can be recorded with great accuracy and spatial resolution, from which the instantaneous vorticity field can be easily obtained. A apparatus used for taking particle displacement images is described. Local coherent illumination by the probe laser beam yielded Young's fringes of good quality at almost every location of the flow field. These fringes were analyzed and the velocity and vorticity fields were derived. Several conclusions drawn are discussed.

Experimental demonstration of Klyshko's advanced-wave picture using a coincidence-count based, camera-enabled imaging system

NASA Astrophysics Data System (ADS)

Aspden, Reuben S.; Tasca, Daniel S.; Forbes, Andrew; Boyd, Robert W.; Padgett, Miles J.

2014-04-01

The Klyshko advanced-wave picture is a well-known tool useful in the conceptualisation of parametric down-conversion (SPDC) experiments. Despite being well-known and understood, there have been few experimental demonstrations illustrating its validity. Here, we present an experimental demonstration of this picture using a time-gated camera in an image-based coincidence measurement. We show an excellent agreement between the spatial distributions as predicted by the Klyshko picture and those obtained using the SPDC photon pairs. An interesting speckle feature is present in the Klyshko predictive images due to the spatial coherence of the back-propagated beam in the multi-mode fibre. This effect can be removed by mechanically twisting the fibre, thus degrading the spatial coherence of the beam and time-averaging the speckle pattern, giving an accurate correspondence between the predictive and SPDC images.

Transient severe left ventricular dysfunction following percutaneous patent ductus arteriosus closure in an adult with bicuspid aortic valve: A case report

PubMed Central

HWANG, HUI-JEONG; YOON, KYUNG LIM; SOHN, IL SUK

2016-01-01

The present study reported the case of a 60-year-old female with patent ductus arteriosus (PDA) and a bicuspid aortic valve, who presented with transient severe left ventricular (LV) dysfunction following percutaneous closure of PDA, as identified by speckle tracking analysis. Transient LV dysfunction following PDA closure has previously been reported; however, severe LV dysfunction is rare. In the present case, the combination of a large PDA size, large amount of shunting, LV remodeling and bicuspid aortic valve may have induced serious deterioration of LV function following PDA closure. Furthermore, speckle-tracking echocardiography may be useful in the estimation of functional alterations in the myocardium of the LV following PDA closure. The observations detailed in the present study may improve the understanding of the pathophysiology and myocardial patterns of transient left ventricular dysfunction following PDA closure in adult humans. PMID:26998021

Transient severe left ventricular dysfunction following percutaneous patent ductus arteriosus closure in an adult with bicuspid aortic valve: A case report.

PubMed

Hwang, Hui-Jeong; Yoon, Kyung Lim; Sohn, Il Suk

2016-03-01

The present study reported the case of a 60-year-old female with patent ductus arteriosus (PDA) and a bicuspid aortic valve, who presented with transient severe left ventricular (LV) dysfunction following percutaneous closure of PDA, as identified by speckle tracking analysis. Transient LV dysfunction following PDA closure has previously been reported; however, severe LV dysfunction is rare. In the present case, the combination of a large PDA size, large amount of shunting, LV remodeling and bicuspid aortic valve may have induced serious deterioration of LV function following PDA closure. Furthermore, speckle-tracking echocardiography may be useful in the estimation of functional alterations in the myocardium of the LV following PDA closure. The observations detailed in the present study may improve the understanding of the pathophysiology and myocardial patterns of transient left ventricular dysfunction following PDA closure in adult humans.

The repeatability and characteristics of right ventricular longitudinal strain imaging by speckle-tracking echocardiography in healthy dogs.

PubMed

Morita, T; Nakamura, K; Osuga, T; Yokoyama, N; Khoirun, N; Morishita, K; Sasaki, N; Ohta, H; Takiguchi, M

2017-08-01

To assess the repeatability and characteristics of echocardiographic indices of the right ventricular (RV) function derived from speckle-tracking echocardiography. Fourteen laboratory Beagles and 103 privately owned dogs without cardiac disease were involved in this study. Right ventricular longitudinal strain, strain rate, and a strain-related index for assessing RV dyssynchrony derived from speckle-tracking echocardiography were obtained by two different observers using five Beagles. Within-day, between-day, and interobserver coefficients of variation and the intraclass correlation coefficient of speckle-tracking echocardiography indices were determined. Both speckle-tracking echocardiography and conventional indices of RV function, including the peak velocity of systolic tricuspid annular motion, tricuspid annulus plane systolic excursion, fractional area change, and the Tei index, were obtained from 14 Beagles and 103 privately owned dogs. Relationships between echocardiographic indices and the body weight, heart rate, age, and sex were estimated by regression analysis. Speckle-tracking echocardiographic indices showed good within-day repeatability, between-day and interobserver repeatability were moderate to good. In large dogs, RV longitudinal strain, strain rate, and fractional area change were significantly decreased, while the index of RV dyssynchrony, systolic tricuspid annular motion, tricuspid annulus plane systolic excursion, and the Tei index were increased. All speckle-tracking and conventional echocardiographic indices were correlated with the body weight. The speckle-tracking echocardiography indices were highly repeatable and body weight affected speckle-tracking echocardiography indices in dogs. Further studies are needed to apply speckle-tracking echocardiography indices in dogs with cardiac disease. Copyright © 2017 Elsevier B.V. All rights reserved.

Speckle averaging system for laser raster-scan image projection

DOEpatents

Tiszauer, D.H.; Hackel, L.A.

1998-03-17

The viewers` perception of laser speckle in a laser-scanned image projection system is modified or eliminated by the addition of an optical deflection system that effectively presents a new speckle realization at each point on the viewing screen to each viewer for every scan across the field. The speckle averaging is accomplished without introduction of spurious imaging artifacts. 5 figs.

Speckle averaging system for laser raster-scan image projection

DOEpatents

Tiszauer, Detlev H.; Hackel, Lloyd A.

1998-03-17

The viewers' perception of laser speckle in a laser-scanned image projection system is modified or eliminated by the addition of an optical deflection system that effectively presents a new speckle realization at each point on the viewing screen to each viewer for every scan across the field. The speckle averaging is accomplished without introduction of spurious imaging artifacts.

Flux or speed? Examining speckle contrast imaging of vascular flows

PubMed Central

Kazmi, S. M. Shams; Faraji, Ehssan; Davis, Mitchell A.; Huang, Yu-Yen; Zhang, Xiaojing J.; Dunn, Andrew K.

2015-01-01

Speckle contrast imaging enables rapid mapping of relative blood flow distributions using camera detection of back-scattered laser light. However, speckle derived flow measures deviate from direct measurements of erythrocyte speeds by 47 ± 15% (n = 13 mice) in vessels of various calibers. Alternatively, deviations with estimates of volumetric flux are on average 91 ± 43%. We highlight and attempt to alleviate this discrepancy by accounting for the effects of multiple dynamic scattering with speckle imaging of microfluidic channels of varying sizes and then with red blood cell (RBC) tracking correlated speckle imaging of vascular flows in the cerebral cortex. By revisiting the governing dynamic light scattering models, we test the ability to predict the degree of multiple dynamic scattering across vessels in order to correct for the observed discrepancies between relative RBC speeds and multi-exposure speckle imaging estimates of inverse correlation times. The analysis reveals that traditional speckle contrast imagery of vascular flows is neither a measure of volumetric flux nor particle speed, but rather the product of speed and vessel diameter. The corrected speckle estimates of the relative RBC speeds have an average 10 ± 3% deviation in vivo with those obtained from RBC tracking. PMID:26203384

Flux or speed? Examining speckle contrast imaging of vascular flows.

PubMed

Kazmi, S M Shams; Faraji, Ehssan; Davis, Mitchell A; Huang, Yu-Yen; Zhang, Xiaojing J; Dunn, Andrew K

2015-07-01

Speckle contrast imaging enables rapid mapping of relative blood flow distributions using camera detection of back-scattered laser light. However, speckle derived flow measures deviate from direct measurements of erythrocyte speeds by 47 ± 15% (n = 13 mice) in vessels of various calibers. Alternatively, deviations with estimates of volumetric flux are on average 91 ± 43%. We highlight and attempt to alleviate this discrepancy by accounting for the effects of multiple dynamic scattering with speckle imaging of microfluidic channels of varying sizes and then with red blood cell (RBC) tracking correlated speckle imaging of vascular flows in the cerebral cortex. By revisiting the governing dynamic light scattering models, we test the ability to predict the degree of multiple dynamic scattering across vessels in order to correct for the observed discrepancies between relative RBC speeds and multi-exposure speckle imaging estimates of inverse correlation times. The analysis reveals that traditional speckle contrast imagery of vascular flows is neither a measure of volumetric flux nor particle speed, but rather the product of speed and vessel diameter. The corrected speckle estimates of the relative RBC speeds have an average 10 ± 3% deviation in vivo with those obtained from RBC tracking.

Compensation for the signal processing characteristics of ultrasound B-mode scanners in adaptive speckle reduction.

PubMed

Crawford, D C; Bell, D S; Bamber, J C

1993-01-01

A systematic method to compensate for nonlinear amplification of individual ultrasound B-scanners has been investigated in order to optimise performance of an adaptive speckle reduction (ASR) filter for a wide range of clinical ultrasonic imaging equipment. Three potential methods have been investigated: (1) a method involving an appropriate selection of the speckle recognition feature was successful when the scanner signal processing executes simple logarithmic compressions; (2) an inverse transform (decompression) of the B-mode image was effective in correcting for the measured characteristics of image data compression when the algorithm was implemented in full floating point arithmetic; (3) characterising the behaviour of the statistical speckle recognition feature under conditions of speckle noise was found to be the method of choice for implementation of the adaptive speckle reduction algorithm in limited precision integer arithmetic. In this example, the statistical features of variance and mean were investigated. The third method may be implemented on commercially available fast image processing hardware and is also better suited for transfer into dedicated hardware to facilitate real-time adaptive speckle reduction. A systematic method is described for obtaining ASR calibration data from B-mode images of a speckle producing phantom.

Speckle interferometry of asteroids

NASA Technical Reports Server (NTRS)

Drummond, Jack

1988-01-01

This final report for NASA Contract NAGw-867 consists of abstracts of the first three papers in a series of four appearing in Icarus that were funded by the preceding contract NAGw-224: (1) Speckle Interferometry of Asteroids I. 433 Eros; (2) Speckle Interferometry of Asteroids II. 532 Herculina; (3) Speckle Interferometry of Asteroids III. 511 Davida and its Photometry; and the fourth abstract attributed to NAGw-867, (4) Speckle Interferometry of Asteroids IV. Reconstructed images of 4 Vesta; and a review of the results from the asteroid interferometry program at Steward Observatory prepared for the Asteroids II book, (5) Speckle Interferometry of Asteroids. Two papers on asteroids, indirectly related to speckle interferometry, were written in part under NAGw-867. One is in press and its abstract is included here: Photometric Geodesy of Main-Belt Asteroids. II. Analysis of Lightcurves for Poles, Periods and Shapes; and the other paper, Triaxial Ellipsoid Dimensions and Rotational Pole of 2 Pallas from Two Stellar Occultations, is included in full.

Speckle tracking as a method to measure hemidiaphragm excursion.

PubMed

Goutman, Stephen A; Hamilton, James D; Swihart, Blake; Foerster, Bradley; Feldman, Eva L; Rubin, Jonathan M

2017-01-01

Diaphragm excursion measured via ultrasound may be an important imaging outcome measure of respiratory function. We developed a new method for measuring diaphragm movement and compared it to the more traditional M-mode method. Ultrasound images of the right and left hemidiaphragms were collected to compare speckle tracking and M-mode measurements of diaphragm excursion. Speckle tracking was performed using EchoInsight (Epsilon Imaging, Ann Arbor, Michigan). Six healthy subjects without a history of pulmonary diseases were included in this proof-of-concept study. Speckle tracking of the diaphragm is technically possible. Unlike M-mode, speckle tracking carries the advantage of reliable visualization and measurement of the left hemidiaphragm. Speckle tracking accounted for diaphragm movement simultaneously in the cephalocaudad and mediolateral directions, unlike M-mode, which is 1-dimensional. Diaphragm speckle tracking may represent a novel, more robust method for measuring diaphragm excursion, especially for the left hemidiaphragm. Muscle Nerve 55: 125-127, 2017. © 2016 Wiley Periodicals, Inc.

Nonlocal Total-Variation-Based Speckle Filtering for Ultrasound Images.

PubMed

Wen, Tiexiang; Gu, Jia; Li, Ling; Qin, Wenjian; Wang, Lei; Xie, Yaoqin

2016-07-01

Ultrasound is one of the most important medical imaging modalities for its real-time and portable imaging advantages. However, the contrast resolution and important details are degraded by the speckle in ultrasound images. Many speckle filtering methods have been developed, but they are suffered from several limitations, difficult to reach a balance between speckle reduction and edge preservation. In this paper, an adaptation of the nonlocal total variation (NLTV) filter is proposed for speckle reduction in ultrasound images. The speckle is modeled via a signal-dependent noise distribution for the log-compressed ultrasound images. Instead of the Euclidian distance, the statistical Pearson distance is introduced in this study for the similarity calculation between image patches via the Bayesian framework. And the Split-Bregman fast algorithm is used to solve the adapted NLTV despeckling functional. Experimental results on synthetic and clinical ultrasound images and comparisons with some classical and recent algorithms are used to demonstrate its improvements in both speckle noise reduction and tissue boundary preservation for ultrasound images. © The Author(s) 2015.

MuLoG, or How to Apply Gaussian Denoisers to Multi-Channel SAR Speckle Reduction?

PubMed

Deledalle, Charles-Alban; Denis, Loic; Tabti, Sonia; Tupin, Florence

2017-09-01

Speckle reduction is a longstanding topic in synthetic aperture radar (SAR) imaging. Since most current and planned SAR imaging satellites operate in polarimetric, interferometric, or tomographic modes, SAR images are multi-channel and speckle reduction techniques must jointly process all channels to recover polarimetric and interferometric information. The distinctive nature of SAR signal (complex-valued, corrupted by multiplicative fluctuations) calls for the development of specialized methods for speckle reduction. Image denoising is a very active topic in image processing with a wide variety of approaches and many denoising algorithms available, almost always designed for additive Gaussian noise suppression. This paper proposes a general scheme, called MuLoG (MUlti-channel LOgarithm with Gaussian denoising), to include such Gaussian denoisers within a multi-channel SAR speckle reduction technique. A new family of speckle reduction algorithms can thus be obtained, benefiting from the ongoing progress in Gaussian denoising, and offering several speckle reduction results often displaying method-specific artifacts that can be dismissed by comparison between results.

Effect of laser speckle on light from laser diode-pumped phosphor-converted light sources.

PubMed

Aquino, Felipe; Jadwisienczak, Wojciech M; Rahman, Faiz

2017-01-10

Laser diode (LD) pumped white light sources are being developed as an alternative to light-emitting diode-pumped sources for high efficiency and/or high brightness applications. While several performance metrics of laser-pumped phosphor-converted light sources have been investigated, the effect of laser speckle has not been sufficiently explored. This paper describes our experimental studies on how laser speckle affects the behavior of light from laser-excited phosphor lamps. A single LD pumping a phosphor plate was the geometry explored in this work. Overall, our findings are that the down-converted light did not exhibit any speckle, whereas speckle was present in the residual pump light but much reduced from that in direct laser light. Furthermore, a thicker coating of small-grained phosphors served to effectively reduce speckle through static pump light diffusion in the phosphor coating. Our investigations showed that speckle is not of concern in illumination from LD-pumped phosphor-converted light sources.

Speckle-reducing scale-invariant feature transform match for synthetic aperture radar image registration

NASA Astrophysics Data System (ADS)

Wang, Xianmin; Li, Bo; Xu, Qizhi

2016-07-01

The anisotropic scale space (ASS) is often used to enhance the performance of a scale-invariant feature transform (SIFT) algorithm in the registration of synthetic aperture radar (SAR) images. The existing ASS-based methods usually suffer from unstable keypoints and false matches, since the anisotropic diffusion filtering has limitations in reducing the speckle noise from SAR images while building the ASS image representation. We proposed a speckle reducing SIFT match method to obtain stable keypoints and acquire precise matches for the SAR image registration. First, the keypoints are detected in a speckle reducing anisotropic scale space constructed by the speckle reducing anisotropic diffusion, so that speckle noise is greatly reduced and prominent structures of the images are preserved, consequently the stable keypoints can be derived. Next, the probabilistic relaxation labeling approach is employed to establish the matches of the keypoints then the correct match rate of the keypoints is significantly increased. Experiments conducted on simulated speckled images and real SAR images demonstrate the effectiveness of the proposed method.

Speckle size in optical Fourier domain imaging

NASA Astrophysics Data System (ADS)

Lamouche, G.; Vergnole, S.; Bisaillon, C.-E.; Dufour, M.; Maciejko, R.; Monchalin, J.-P.

2007-06-01

As in conventional time-domain optical coherence tomography (OCT), speckle is inherent to any Optical Fourier Domain Imaging (OFDI) of biological tissue. OFDI is also known as swept-source OCT (SS-OCT). The axial speckle size is mainly determined by the OCT resolution length and the transverse speckle size by the focusing optics illuminating the sample. There is also a contribution from the sample related to the number of scatterers contained within the probed volume. In the OFDI data processing, there is some liberty in selecting the range of wavelengths used and this allows variation in the OCT resolution length. Consequently the probed volume can be varied. By performing measurements on an optical phantom with a controlled density of discrete scatterers and by changing the probed volume with different range of wavelengths in the OFDI data processing, there is an obvious change in the axial speckle size, but we show that there is also a less obvious variation in the transverse speckle size. This work contributes to a better understanding of speckle in OCT.

Correction for spatial averaging in laser speckle contrast analysis

PubMed Central

Thompson, Oliver; Andrews, Michael; Hirst, Evan

2011-01-01

Practical laser speckle contrast analysis systems face a problem of spatial averaging of speckles, due to the pixel size in the cameras used. Existing practice is to use a system factor in speckle contrast analysis to account for spatial averaging. The linearity of the system factor correction has not previously been confirmed. The problem of spatial averaging is illustrated using computer simulation of time-integrated dynamic speckle, and the linearity of the correction confirmed using both computer simulation and experimental results. The valid linear correction allows various useful compromises in the system design. PMID:21483623

Laser speckle technology in stomatology. diagnostics of stresses and strains of hard biotissues and orthodontic and orthopedic structures

NASA Astrophysics Data System (ADS)

Denisova, Yu. L.; Bazylev, N. B.; Rubnikovich, S. P.; Fomin, N. A.

2013-07-01

We have investigated the formation and dynamics of speckle biofi elds formed by hard biotissues of the oral cavity irradiated with low-intensity radiation. We present experimental methods for diagnosing the stressed-strained state of the maxillodental system and orthodontic and orthopedic structures based on speckle technologies and crosscorrelation analysis of speckle biofi elds.