Altered vision destabilizes gait in older persons.

PubMed

Helbostad, Jorunn L; Vereijken, Beatrix; Hesseberg, Karin; Sletvold, Olav

2009-08-01

This study assessed the effects of dim light and four experimentally induced changes in vision on gait speed and footfall and trunk parameters in older persons walking on level ground. Using a quasi-experimental design, gait characteristics were assessed in full light, dim light, and in dim light combined with manipulations resulting in reduced depth vision, double vision, blurred vision, and tunnel vision, respectively. A convenience sample of 24 home-dwelling older women and men (mean age 78.5 years, SD 3.4) with normal vision for their age and able to walk at least 10 m without assistance participated. Outcome measures were gait speed and spatial and temporal parameters of footfall and trunk acceleration, derived from an electronic gait mat and accelerometers. Dim light alone had no effect. Vision manipulations combined with dim light had effect on most footfall parameters but few trunk parameters. The largest effects were found regarding double and tunnel vision. Men increased and women decreased gait speed following manipulations (p=0.017), with gender differences also in stride velocity variability (p=0.017) and inter-stride medio-lateral trunk acceleration variability (p=0.014). Gender effects were related to differences in body height and physical functioning. Results indicate that visual problems lead to a more cautious and unstable gait pattern even under relatively simple conditions. This points to the importance of assessing vision in older persons and correcting visual impairments where possible.

Dim light at night disrupts molecular circadian rhythms and increases body weight.

PubMed

Fonken, Laura K; Aubrecht, Taryn G; Meléndez-Fernández, O Hecmarie; Weil, Zachary M; Nelson, Randy J

2013-08-01

With the exception of high latitudes, life has evolved under bright days and dark nights. Most organisms have developed endogenously driven circadian rhythms that are synchronized to this daily light/dark cycle. In recent years, humans have shifted away from the naturally occurring solar light cycle in favor of artificial and sometimes irregular light schedules produced by electric lighting. Exposure to unnatural light cycles is increasingly associated with obesity and metabolic syndrome; however, the means by which environmental lighting alters metabolism are poorly understood. Thus, we exposed mice to dim light at night and investigated changes in the circadian system and metabolism. Here we report that exposure to ecologically relevant levels of dim (5 lux) light at night altered core circadian clock rhythms in the hypothalamus at both the gene and protein level. Circadian rhythms in clock expression persisted during light at night; however, the amplitude of Per1 and Per2 rhythms was attenuated in the hypothalamus. Circadian oscillations were also altered in peripheral tissues critical for metabolic regulation. Exposure to dimly illuminated, as compared to dark, nights decreased the rhythmic expression in all but one of the core circadian clock genes assessed in the liver. Additionally, mice exposed to dim light at night attenuated Rev-Erb expression in the liver and adipose tissue. Changes in the circadian clock were associated with temporal alterations in feeding behavior and increased weight gain. These results are significant because they provide evidence that mild changes in environmental lighting can alter circadian and metabolic function. Detailed analysis of temporal changes induced by nighttime light exposure may provide insight into the onset and progression of obesity and metabolic syndrome, as well as other disorders involving sleep and circadian rhythm disruption.

Signal coding in cockroach photoreceptors is tuned to dim environments.

PubMed

Heimonen, K; Immonen, E-V; Frolov, R V; Salmela, I; Juusola, M; Vähäsöyrinki, M; Weckström, M

2012-11-01

In dim light, scarcity of photons typically leads to poor vision. Nonetheless, many animals show visually guided behavior with dim environments. We investigated the signaling properties of photoreceptors of the dark active cockroach (Periplaneta americana) using intracellular and whole-cell patch-clamp recordings to determine whether they show selective functional adaptations to dark. Expectedly, dark-adapted photoreceptors generated large and slow responses to single photons. However, when light adapted, responses of both phototransduction and the nontransductive membrane to white noise (WN)-modulated stimuli remained slow with corner frequencies ~20 Hz. This promotes temporal integration of light inputs and maintains high sensitivity of vision. Adaptive changes in dynamics were limited to dim conditions. Characteristically, both step and frequency responses stayed effectively unchanged for intensities >1,000 photons/s/photoreceptor. A signal-to-noise ratio (SNR) of the light responses was transiently higher at frequencies <5 Hz for ~5 s after light onset but deteriorated to a lower value upon longer stimulation. Naturalistic light stimuli, as opposed to WN, evoked markedly larger responses with higher SNRs at low frequencies. This allowed realistic estimates of information transfer rates, which saturated at ~100 bits/s at low-light intensities. We found, therefore, selective adaptations beneficial for vision in dim environments in cockroach photoreceptors: large amplitude of single-photon responses, constant high level of temporal integration of light inputs, saturation of response properties at low intensities, and only transiently efficient encoding of light contrasts. The results also suggest that the sources of the large functional variability among different photoreceptors reside mostly in phototransduction processes and not in the properties of the nontransductive membrane.

Dim Light at Night Increases Body Mass of Female Mice

PubMed Central

Aubrecht, Taryn G.; Jenkins, Richelle; Nelson, Randy J.

2016-01-01

During the past century the prevalence of light at night has increased in parallel with obesity rates. Dim light at night (dLAN) increases body mass in male mice. However, the effects of light at night on female body mass remain unspecified. Thus, female mice were exposed to a standard light/dark (LD; 16h light at ~150 lux/8h dark at ~0 lux) cycle or to light/dim light at night (dLAN; 16h light at ~150 lux/8h dim light at ~5 lux) cycles for six weeks. Females exposed to dLAN increased the rate of change in body mass compared to LD mice despite reduced total food intake during weeks five and six, suggesting that dLAN disrupted circadian rhythms resulting in deranged metabolism. PMID:25431079

Dim light at night increases body mass of female mice.

PubMed

Aubrecht, Taryn G; Jenkins, Richelle; Nelson, Randy J

2015-05-01

During the past century, the prevalence of light at night has increased in parallel with obesity rates. Dim light at night (dLAN) increases body mass in male mice. However, the effects of light at night on female body mass remain unspecified. Thus, female mice were exposed to a standard light/dark (LD; 16 h light at ∼150 lux/8 h dark at ∼0 lux) cycle or to light/dim light at night (dLAN; 16 h light at ∼150 lux/8 h dim light at ∼5 lux) cycles for six weeks. Females exposed to dLAN increased the rate of change in body mass compared to LD mice despite reduced total food intake during weeks five and six, suggesting that dLAN disrupted circadian rhythms resulting in deranged metabolism.

Reproducibility of an objective four-choice canine vision testing technique that assesses vision at differing light intensities.

PubMed

Annear, Matthew J; Gornik, Kara R; Venturi, Francesca L; Hauptman, Joe G; Bartoe, Joshua T; Petersen-Jones, Simon M

2013-09-01

The increasing importance of canine retinal dystrophy models means accurate vision testing is needed. This study was performed to evaluate a four-choice vision testing technique for any difference in outcome measures with repeated evaluations of the same dogs.   Four 11-month-old RPE65-deficient dogs. Vision was evaluated using a previously described four-choice vision testing device. Four evaluations were performed at 2-week intervals. Vision was assessed at six different white light intensities (bright through dim), and each eye was evaluated separately. The ability to select the one of the four exit tunnels that was open at the far end was assessed ('choice of exit') and recorded as correct or incorrect first tunnel choice. 'Time to exit' the device was also recorded. Both outcomes were analyzed for significance using anova. We hypothesized that performance would improve with repeated testing (more correct choices and more rapid time to exit). 'Choice of exit' did not vary significantly between each evaluation (P = 0.12), in contrast 'time to exit' increased significantly (P = 0.012), and showed greater variability in dim light conditions. We found no evidence to support the hypothesis that either measure of outcome worsened with repeated testing; in fact, the 'time to exit' outcome worsened rather than improved. The 'choice of exit' gave consistent results between trials. These outcome data indicate the importance of including a choice-based assessment of vision in addition to measurement of device transit time. © 2012 American College of Veterinary Ophthalmologists.

Behavioural environments and niche construction: the evolution of dim-light foraging in bees.

PubMed

Wcislo, William T; Tierney, Simon M

2009-02-01

Most bees forage for floral resources during the day, but temporal patterns of foraging activity vary extensively, and foraging in dim-light environments has evolved repeatedly. Facultative dim-light foraging behaviour is known in five of nine families of bees, while obligate behaviour is known in four families and evolved independently at least 19 times. The light intensity under which bees forage varies by a factor of 10(8), and therefore the evolution of dim-light foraging represents the invasion of a new, extreme niche. The repeated evolution of dim-light foraging behaviour in bees allows tests of the hypothesis that behaviour acts as an evolutionary pacemaker. With the exception of one species of Apis, facultative dim-light foragers show no external structural traits that are thought to enable visually mediated flight behaviour in low-light environments. By contrast, most obligate dim-light foragers show a suite of convergent optical traits such as enlarged ocelli and compound eyes. In one intensively studied species (Megalopta genalis) these optical changes are associated with neurobiological changes to enhance photon capture. The available ecological evidence suggests that an escape from competition for pollen and nectar resources and avoidance of natural enemies are driving factors in the evolution of obligate dim-light foraging.

Photic niche invasions: phylogenetic history of the dim-light foraging augochlorine bees (Halictidae)

PubMed Central

Tierney, Simon M.; Sanjur, Oris; Grajales, Grethel G.; Santos, Leandro M.; Bermingham, Eldredge; Wcislo, William T.

2012-01-01

Most bees rely on flowering plants and hence are diurnal foragers. From this ancestral state, dim-light foraging in bees requires significant adaptations to a new photic environment. We used DNA sequences to evaluate the phylogenetic history of the most diverse clade of Apoidea that is adapted to dim-light environments (Augochlorini: Megalopta, Megaloptidia and Megommation). The most speciose lineage, Megalopta, is distal to the remaining dim-light genera, and its closest diurnal relative (Xenochlora) is recovered as a lineage that has secondarily reverted to diurnal foraging. Tests for adaptive protein evolution indicate that long-wavelength opsin shows strong evidence of stabilizing selection, with no more than five codons (2%) under positive selection, depending on analytical procedure. In the branch leading to Megalopta, the amino acid of the single positively selected codon is conserved among ancestral Halictidae examined, and is homologous to codons known to influence molecular structure at the chromophore-binding pocket. Theoretically, such mutations can shift photopigment λmax sensitivity and enable visual transduction in alternate photic environments. Results are discussed in light of the available evidence on photopigment structure, morphological specialization and biogeographic distributions over geological time. PMID:21795273

Photic niche invasions: phylogenetic history of the dim-light foraging augochlorine bees (Halictidae).

PubMed

Tierney, Simon M; Sanjur, Oris; Grajales, Grethel G; Santos, Leandro M; Bermingham, Eldredge; Wcislo, William T

2012-02-22

Most bees rely on flowering plants and hence are diurnal foragers. From this ancestral state, dim-light foraging in bees requires significant adaptations to a new photic environment. We used DNA sequences to evaluate the phylogenetic history of the most diverse clade of Apoidea that is adapted to dim-light environments (Augochlorini: Megalopta, Megaloptidia and Megommation). The most speciose lineage, Megalopta, is distal to the remaining dim-light genera, and its closest diurnal relative (Xenochlora) is recovered as a lineage that has secondarily reverted to diurnal foraging. Tests for adaptive protein evolution indicate that long-wavelength opsin shows strong evidence of stabilizing selection, with no more than five codons (2%) under positive selection, depending on analytical procedure. In the branch leading to Megalopta, the amino acid of the single positively selected codon is conserved among ancestral Halictidae examined, and is homologous to codons known to influence molecular structure at the chromophore-binding pocket. Theoretically, such mutations can shift photopigment λ(max) sensitivity and enable visual transduction in alternate photic environments. Results are discussed in light of the available evidence on photopigment structure, morphological specialization and biogeographic distributions over geological time.

Light-dependent magnetoreception: orientation behaviour of migratory birds under dim red light.

PubMed

Wiltschko, Roswitha; Munro, Ursula; Ford, Hugh; Stapput, Katrin; Wiltschko, Wolfgang

2008-10-01

Magnetic compass orientation in migratory birds has been shown to be based on radical pair processes and to require light from the short wavelength part of the spectrum up to 565 nm Green. Under dim red light of 645 nm wavelength and 1 mW m(-2) intensity, Australian silvereyes and European robins showed a westerly tendency that did not change between spring and autumn, identifying it as a 'fixed direction' response. A thorough analysis revealed that this orientation did not involve the inclination compass, but was a response based on the polarity of the magnetic field. Furthermore, in contrast to the orientation under short-wavelength light, it could be disrupted by local anaesthesia of the upper beak where iron-containing receptors are located, indicating that it is controlled by these receptors. The similarity of the response under dim red light to the response in total darkness suggests that the two responses may be identical. These findings indicate that the observed 'fixed direction' response under dim red light is fundamentally different from the normal compass orientation, which is based on radical pair processes.

Dim ultraviolet light as a means of deterring activity by the Hawaiian hoary bat Lasiurus cinereus semotus

USGS Publications Warehouse

Gorresen, P. Marcos; Cryan, Paul M.; Dalton, David C.; Wolf, Sandy; Johnson, Jessica A.; Todd, Christopher M.; Bonaccorso, Frank J.

2015-01-01

Widespread bat fatalities at industrial wind turbines are a conservation issue with the potential to inhibit efficient use of an abundant source of energy. Bat fatalities can be reduced by altering turbine operations, but such curtailment decreases turbine efficiency. If additional ways of reducing bat fatalities at wind turbines were available such tradeoffs might not be needed. Based on the facts that bats perceive distant objects primarily through vision and can see in very dim lighting conditions, and the possibility that bats might interact with turbines after approaching them as they would trees, we propose a novel method of reducing bat activity at wind turbines: illumination of the structure with dim light. As a first step toward assessing this approach, we illuminated trees with dim flickering ultraviolet (UV) light in areas frequented by Hawaiian hoary bats Lasiurus cinereus semotus, an endangered subspecies affected by wind turbines. We used a repeated-measures design to quantify bat activity near trees with acoustic detectors and thermal video cameras in the presence and absence of UV illumination, while concurrently monitoring insect numbers. Results indicate that dim UV reduces bat activity despite an increase in insect numbers. Experimental treatment did not completely inhibit bat activity near trees, nor did all measures of bat activity show statistically significant differences due to high variance in bat activity among sites. However, the observed decreases in bat activity with dim UV illumination justify further testing of this method as a means to reduce bat fatalities at wind turbines.

Effects of different light intensities in the morning on dim light melatonin onset.

PubMed

Kozaki, Tomoaki; Toda, Naohiro; Noguchi, Hiroki; Yasukouchi, Akira

2011-01-01

The present study evaluated the effects of exposure to light intensity in the morning on dim light melatonin onset (DLMO). The tested light intensities were 750 lux, 150 lux, 3000 lux, 6000 lux and 12,000 lux (horizontal illuminance at cornea), using commercial 5000 K fluorescent lamps. Eleven healthy males aged 21-31 participated in 2-day experiments for each light condition. On the first experimental day (day 1), subjects were exposed to dim light (<30 lux) for 3 h in the morning (09:00-12:00). On the same day, saliva samples were taken in dim light (<30 lux) every 30 min from 21:00 to 01:00 to determine the DLMO phase. The subjects were allowed to sleep from 01:00 to 08:00. On the second experimental day (day 2), the subjects were exposed to experimental light conditions for 3 h in the morning. The experimental schedule after light exposure was the same as on day 1. On comparing day 2 with day 1, significant phase advances of DLMO were obtained at 3000 lux, 6000 lux and 12,000 lux. These findings indicate that exposure to a necessary intensity from an ordinary light source, such as a fluorescent lamp, in the morning within one day affects melatonin secretion.

Body size limits dim-light foraging activity in stingless bees (Apidae: Meliponini).

PubMed

Streinzer, Martin; Huber, Werner; Spaethe, Johannes

2016-10-01

Stingless bees constitute a species-rich tribe of tropical and subtropical eusocial Apidae that act as important pollinators for flowering plants. Many foraging tasks rely on vision, e.g. spatial orientation and detection of food sources and nest entrances. Meliponini workers are usually small, which sets limits on eye morphology and thus quality of vision. Limitations are expected both on acuity, and thus on the ability to detect objects from a distance, as well as on sensitivity, and thus on the foraging time window at dusk and dawn. In this study, we determined light intensity thresholds for flight under dim light conditions in eight stingless bee species in relation to body size in a Neotropical lowland rainforest. Species varied in body size (0.8-1.7 mm thorax-width), and we found a strong negative correlation with light intensity thresholds (0.1-79 lx). Further, we measured eye size, ocelli diameter, ommatidia number, and facet diameter. All parameters significantly correlated with body size. A disproportionately low light intensity threshold in the minute Trigonisca pipioli, together with a large eye parameter P eye suggests specific adaptations to circumvent the optical constraints imposed by the small body size. We discuss the implications of body size in bees on foraging behavior.

Living in the dark does not mean a blind life: bird and mammal visual communication in dim light.

PubMed

Penteriani, Vincenzo; Delgado, María Del Mar

2017-04-05

For many years, it was believed that bird and mammal communication 'in the dark of the night' relied exclusively on vocal and chemical signalling. However, in recent decades, several case studies have conveyed the idea that the nocturnal world is rich in visual information. Clearly, a visual signal needs a source of light to work, but diurnal light (twilight included, i.e. any light directly dependent on the sun) is not the only source of luminosity on this planet. Actually, moonlight represents a powerful source of illumination that cannot be neglected from the perspective of visual communication. White patches of feathers and fur on a dark background have the potential to be used to communicate with conspecifics and heterospecifics in dim light across different contexts and for a variety of reasons. Here: (i) we review current knowledge on visual signalling in crepuscular and nocturnal birds and mammals; and (ii) we also present some possible cases of birds and mammals that, due to the characteristics of their feather and fur coloration pattern, might use visual signals in dim light. Visual signalling in nocturnal animals is still an emerging field and, to date, it has received less attention than many other means of communication, including visual communication under daylight. For this reason, many questions remain unanswered and, sometimes, even unasked.This article is part of the themed issue 'Vision in dim light'. © 2017 The Author(s).

Supplementary dim light differentially influences sexual maturity, oviposition time, and melatonin rhythms in pullets.

PubMed

Lewis, P D; Perry, G C; Morris, T R; English, J

2001-12-01

The addition of two 3-h periods of very dim light, one before and one after a normal 8-h photoperiod, advances sexual maturity in pullets by about a week. This trial tested the hypothesis that dim light given before a short day of normal intensity is linked to form a more stimulatory day length and that dim light given after it is photosexually ignored. Pullets were reared from 2 d of age on 8-h photoperiods. From 10 wk, they were continued on 8-h photoperiods, transferred to 16 h, or given an 8-h period of dim light (0.09 lx) immediately before or after the main 8-h photoperiod. The bright/dim and dim/ bright groups matured at the same age, thus disproving the hypothesis tested. Both groups matured 1 wk earlier than the 8-h controls but 5 wk later than birds transferred to 16-h photoperiod. Oviposition time was similar for 8-h controls and bright/dim hens and delayed by 3 h for 16-h birds, but phase advanced by 2.4 h for dim/bright hens. Plasma melatonin rhythm was phase-advanced by about 5 h in the dim/bright hens and retarded by about 5 h in the bright/dim hens, suggesting a 13-h subjective day. However, these treatments were not regarded as fully stimulatory, as a transfer to a normal 13-h photoperiod at this age advances maturity by 5 to 6 wk. These findings show that the addition of a period of dim light to a normal nonstimulatory photoperiod differentially affects the clocks that control sexual maturation, plasma melatonin concentration, and oviposition time.

Field Commissioning of a Daylight-Dimming Lighting System.

ERIC Educational Resources Information Center

Floyd, David B.; Parker, Danny S.

A Florida elementary school cafeteria, retrofitted with a fluorescent lighting system that dims in response to available daylight, was evaluated through real time measurement of lighting and air conditioning power, work plane illumination, and interior/exterior site conditions. The new system produced a 27 percent reduction in lighting power due…

Entrainment of oviposition in the fowl using bright and dim light cycles.

PubMed

Morris, T R; Bhatti, B M

1978-05-01

1. Nine short trial, involving 96 different treatments, were used to investigate the critical intensities and duration of bright and dim periods of lighting needed to entrain oviposition in cycles ranging from 21 to 30 h. 2. Entrainment was shown to depend upon the contrast between bright and dim lighting, and to be independent of the absolute light intensity. 3. A bright: dim ratio of 13:1 fully entrained oviposition in cycles of 25 h and 27 h. For 23-h and 28-h cycles a 30:1 ratio was required. Twenty-one-hour cycles required a ratio of 300:1 and with 30-h cycles a ratio of 1000:1 was needed to achieve full entrainment of oviposition. 4. In 24-h cycles, 1 h of bright lighting at 02.00 h was sufficient to override other environmental signals and cause eggs to be laid in the late evening, but a minimum bright period of 6 h was needed to cause full phase setting with 21-h cycles. 5. Circadian periodicity can easily be imposed on hens by providing a short exposure to bright light with a background of continuous dim light; but the signal must be increased (by providing a greater contrast between bright and dim lights and/or a longer period of bright lighting) to entrain oviposition when the cycle deviates markedly from the natural period of 24 h.

Living in the dark does not mean a blind life: bird and mammal visual communication in dim light

PubMed Central

2017-01-01

For many years, it was believed that bird and mammal communication ‘in the dark of the night’ relied exclusively on vocal and chemical signalling. However, in recent decades, several case studies have conveyed the idea that the nocturnal world is rich in visual information. Clearly, a visual signal needs a source of light to work, but diurnal light (twilight included, i.e. any light directly dependent on the sun) is not the only source of luminosity on this planet. Actually, moonlight represents a powerful source of illumination that cannot be neglected from the perspective of visual communication. White patches of feathers and fur on a dark background have the potential to be used to communicate with conspecifics and heterospecifics in dim light across different contexts and for a variety of reasons. Here: (i) we review current knowledge on visual signalling in crepuscular and nocturnal birds and mammals; and (ii) we also present some possible cases of birds and mammals that, due to the characteristics of their feather and fur coloration pattern, might use visual signals in dim light. Visual signalling in nocturnal animals is still an emerging field and, to date, it has received less attention than many other means of communication, including visual communication under daylight. For this reason, many questions remain unanswered and, sometimes, even unasked. This article is part of the themed issue ‘Vision in dim light’. PMID:28193809

Daytime exposure to bright light, as compared to dim light, decreases sleepiness and improves psychomotor vigilance performance.

PubMed

Phipps-Nelson, Jo; Redman, Jennifer R; Dijk, Derk-Jan; Rajaratnam, Shantha M W

2003-09-01

This study examined the effects of bright light exposure, as compared to dim light, on daytime subjective sleepiness, incidences of slow eye movements (SEMs), and psychomotor vigilance task (PVT) performance following 2 nights of sleep restriction. The study had a mixed factorial design with 2 independent variables: light condition (bright light, 1,000 lux; dim light, < 5 lux) and time of day. The dependent variables were subjective sleepiness, PVT performance, incidences of SEMs, and salivary melatonin levels. Sleep research laboratory at Monash University. Sixteen healthy adults (10 women and 6 men) aged 18 to 35 years (mean age 25 years, 3 months). Following 2 nights of sleep restriction (5 hours each night), participants were exposed to modified constant routine conditions. Eight participants were exposed to bright light from noon until 5:00 pm. Outside the bright light exposure period (9:00 am to noon, 5:00 pm to 9:00 pm) light levels were maintained at less than 5 lux. A second group of 8 participants served as controls for the bright light exposure and were exposed to dim light throughout the entire protocol. Bright light exposure reduced subjective sleepiness, decreased SEMs, and improved PVT performance compared to dim light. Bright lights had no effect on salivary melatonin. A significant positive correlation between PVT reaction times and subjective sleepiness was observed for both groups. Changes in SEMs did not correlate significantly with either subjective sleepiness or PVT performance. Daytime bright light exposure can reduce the impact of sleep loss on sleepiness levels and performance, as compared to dim light. These effects appear to be mediated by mechanisms that are separate from melatonin suppression. The results may assist in the development of treatments for daytime sleepiness.

Short-term exposure to dim light at night disrupts rhythmic behaviors and causes neurodegeneration in fly models of tauopathy and Alzheimer's disease.

PubMed

Kim, Mari; Subramanian, Manivannan; Cho, Yun-Ho; Kim, Gye-Hyeong; Lee, Eunil; Park, Joong-Jean

2018-01-08

The accumulation and aggregation of phosphorylated tau proteins in the brain are the hallmarks for the onset of Alzheimer's disease (AD). In addition, disruptions in circadian rhythms (CRs) with altered sleep-wake cycles, dysregulation of locomotion, and increased memory defects have been reported in patients with AD. Drosophila flies that have an overexpression of human tau protein in neurons exhibit most of the symptoms of human patients with AD, including locomotion defects and neurodegeneration. Using the fly model for tauopathy/AD, we investigated the effects of an exposure to dim light at night on AD symptoms. We used a light intensity of 10 lux, which is considered the lower limit of light pollution in many countries. After the tauopathy flies were exposed to the dim light at night for 3 days, the flies showed disrupted CRs, altered sleep-wake cycles due to increased pTau proteins and neurodegeneration, in the brains of the AD flies. The results indicate that the nighttime exposure of tauopathy/AD model Drosophila flies to dim light disrupted CR and sleep-wake behavior and promoted neurodegeneration. Copyright © 2017 Elsevier Inc. All rights reserved.

Effect of housing rats in dim light or long nights on heart rate.

PubMed

Azar, Toni A; Sharp, Jody L; Lawson, David M

2008-07-01

Housing laboratory animals under lighting conditions that more closely mimic the natural environment may improve their wellbeing. This study examined the effects of dim light or a long-night photocycle on resting heart rate (HR) of rats and their HR responses to acute procedures. Male and female Sprague-Dawley (SD) and spontaneously hypertensive (SHR) rats, instrumented with radiotelemetry transmitters and housed individually under a 12:12-h light:dark photocycle with 10 lx illumination (dim light) or under an 8:16-h light:dark photocycle with 200 lx illumination (long nights), were compared with control rats individually housed under a 12:12-h light:dark photocycle with 200 lx illumination. Dim light and long nights significantly reduced the HR of undisturbed SD and SHR male and SHR female rats during the day and at night; however, the HR of undisturbed SD females was not affected. When rats were subjected acutely to husbandry, experimental, or stressful procedures, dim light or long nights (or both) reduced HR responses to some procedures, did not alter responses to others, and increased responses to yet other procedures. The pattern of effects varied between strains and between male and female rats. Because basal HR was reduced when rats were housed under 10 lx illumination or an 8:16-h light:dark photocycle, we concluded that housing rats under 12:12-h light:dark, 200 lx ambient light conditions was potentially stressful, We also concluded that dim light or long nights did not uniformly reduce the increased HR responses induced by acute procedures.

Effects of dim or bright-light exposure during the daytime on human gastrointestinal activity.

PubMed

Sone, Yoshiaki; Hyun, Ki-Ja; Nishimura, Shinya; Lee, Young-Ah; Tokura, Hiromi

2003-01-01

On the basis of our previous findings that bright-light exposure during the daytime has profound influence on physiological parameters such as melatonin secretion and tympanic temperature in humans, we proposed the hypothesis that bright vs. dim light-exposure during the daytime has a different influence on the activity of the digestive system via the endocrine and/or autonomic nervous system. To examine this hypothesis, we conducted a series of counterbalanced experiments in which subjects stayed the daytime (7:00 to 15:00h) under either a dim (80 lux) or bright (5,000 lux) light condition. We measured gastrointestinal activity using a breath hydrogen (indicative of carbohydrate malabsorption) and an electrogastrography (EGG, indicative of gastric myoelectric activity) test. The results showed the postprandial breath hydrogen excretion during the following nighttime period after daytime exposure to the dim-light condition was significantly higher than under the bright-light condition (p < 0.05). In addition, the spectrum total power of the EGG recorded after taking the evening meal was significantly lower for the dim than bright-light condition (p < 0.05). These results support our hypothesis and indicate that dim-light exposure during the daytime suppresses the digestion of the evening meal, resulting in malabsorption of dietary carbohydrates in it.

Invasion of Ancestral Mammals into Dim-light Environments Inferred from Adaptive Evolution of the Phototransduction Genes.

PubMed

Wu, Yonghua; Wang, Haifeng; Hadly, Elizabeth A

2017-04-20

Nocturnality is a key evolutionary innovation of mammals that enables mammals to occupy relatively empty nocturnal niches. Invasion of ancestral mammals into nocturnality has long been inferred from the phylogenetic relationships of crown Mammalia, which is primarily nocturnal, and crown Reptilia, which is primarily diurnal, although molecular evidence for this is lacking. Here we used phylogenetic analyses of the vision genes involved in the phototransduction pathway to predict the diel activity patterns of ancestral mammals and reptiles. Our results demonstrated that the common ancestor of the extant Mammalia was dominated by positive selection for dim-light vision, supporting the predominate nocturnality of the ancestral mammals. Further analyses showed that the nocturnality of the ancestral mammals was probably derived from the predominate diurnality of the ancestral amniotes, which featured strong positive selection for bright-light vision. Like the ancestral amniotes, the common ancestor of the extant reptiles and various taxa in Squamata, one of the main competitors of the temporal niches of the ancestral mammals, were found to be predominate diurnality as well. Despite this relatively apparent temporal niche partitioning between ancestral mammals and the relevant reptiles, our results suggested partial overlap of their temporal niches during crepuscular periods.

A late wake time phase delays the human dim light melatonin rhythm.

PubMed

Burgess, Helen J; Eastman, Charmane I

2006-03-13

Short sleep/dark durations, due to late bedtimes or early wake times or both, are common in modern society. We have previously shown that a series of days with a late bedtime phase delays the human dim light melatonin rhythm, as compared to a series of days with an early bedtime, despite a fixed wake time. Here we compared the effect of an early versus late wake time with a fixed bedtime on the human dim light melatonin rhythm. Fourteen healthy subjects experienced 2 weeks of short 6h nights with an early wake time fixed at their habitual weekday wake time and 2 weeks of long 9 h nights with a wake time that occurred 3h later than the early wake time, in counterbalanced order. We found that after 2 weeks with the late wake time, the dim light melatonin onset delayed by 2.4 h and the dim light melatonin offset delayed by 2.6 h (both p < 0.001), as compared to after 2 weeks with the early wake time. These results highlight the substantial influence that wake time, likely via the associated morning light exposure, has on the timing of the human circadian clock. Furthermore, the results suggest that when people truncate their sleep by waking early their circadian clocks phase advance and when people wake late their circadian clocks phase delay.

Accelerated Evolution and Functional Divergence of the Dim Light Visual Pigment Accompanies Cichlid Colonization of Central America.

PubMed

Hauser, Frances E; Ilves, Katriina L; Schott, Ryan K; Castiglione, Gianni M; López-Fernández, Hernán; Chang, Belinda S W

2017-10-01

Cichlids encompass one of the most diverse groups of fishes in South and Central America, and show extensive variation in life history, morphology, and colouration. While studies of visual system evolution in cichlids have focussed largely on the African rift lake species flocks, Neotropical cichlids offer a unique opportunity to investigate visual system evolution at broader temporal and geographic scales. South American cichlid colonization of Central America has likely promoted accelerated rates of morphological evolution in Central American lineages as they encountered reduced competition, renewed ecological opportunity, and novel aquatic habitats. To investigate whether such transitions have influenced molecular evolution of vision in Central American cichlids, we sequenced the dim-light rhodopsin gene in 101 Neotropical cichlid species, spanning the diversity of the clade. We find strong evidence for increased rates of evolution in Central American cichlid rhodopsin relative to South American lineages, and identify several sites under positive selection in rhodopsin that likely contribute to adaptation to different photic environments. We expressed a Neotropical cichlid rhodopsin protein invitro for the first time, and found that while its spectral tuning properties were characteristic of typical vertebrate rhodopsin pigments, the rate of decay of its active signalling form was much slower, consistent with dim light adaptation in other vertebrate rhodopsins. Using site-directed mutagenesis combined with spectroscopic assays, we found that a key amino acid substitution present in some Central American cichlids accelerates the rate of decay of active rhodopsin, which may mediate adaptation to clear water habitats. © The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Ultraviolet vision may be widespread in bats

USGS Publications Warehouse

Gorresen, P. Marcos; Cryan, Paul; Dalton, David C.; Wolf, Sandy; Bonaccorso, Frank

2015-01-01

Insectivorous bats are well known for their abilities to find and pursue flying insect prey at close range using echolocation, but they also rely heavily on vision. For example, at night bats use vision to orient across landscapes, avoid large obstacles, and locate roosts. Although lacking sharp visual acuity, the eyes of bats evolved to function at very low levels of illumination. Recent evidence based on genetics, immunohistochemistry, and laboratory behavioral trials indicated that many bats can see ultraviolet light (UV), at least at illumination levels similar to or brighter than those before twilight. Despite this growing evidence for potentially widespread UV vision in bats, the prevalence of UV vision among bats remains unknown and has not been studied outside of the laboratory. We used a Y-maze to test whether wild-caught bats could see reflected UV light and whether such UV vision functions at the dim lighting conditions typically experienced by night-flying bats. Seven insectivorous species of bats, representing five genera and three families, showed a statistically significant ‘escape-toward-the-light’ behavior when placed in the Y-maze. Our results provide compelling evidence of widespread dim-light UV vision in bats.

[Study on Hexagonal Super-Lattice Pattern with Light Spot and Dim Spot in Dielectric Barrier Discharge by Optical Emission Spectra].

PubMed

Liu, Ying; Dong, Li-fang; Niu, Xue-jiao; Zhang, Chao

2016-02-01

The hexagonal super-lattice pattern composed of the light spot and the dim spot is firstly observed and investigated in the discharge of gas mixture of air and argon by using the dielectric barrier discharge device with double water electrodes. It is found that the dim spot is located at the center of its surrounding three light spots by observing the discharge image. Obviously, the brightness of the light spot and the dim spot are different, which indicates that the plasma states of the light spot and the dim spot may be different. The optical emission spectrum method is used to further study the several plasma parameters of the light spot and the dim spot in different argon content. The emission spectra of the N₂ second positive band (C³IIu --> B³IIg) are measured, from which the molecule vibration temperatures of the light spot and the dim spot are calculated. Based on the relative intensity ratio of the line at 391.4 nm and the N₂ line at 394.1 nm, the average electron energies of the light spot and the dim spot are investigated. The broadening of spectral line 696.57 nm (2P₂-1S₅) is used to study the electron densities of the light spot and the dim spot. The experiment shows that the molecule vibration temperature, average electron energy and the electron density of the dim spot are higher than those of the light spot in the same argon content. The molecule vibration temperature and electron density of the light spot and dim spot increase with the argon content increasing from 70% to 95%, while average electron energies of the light spot and dim spot decrease gradually. The short-exposure image recorded by a high speed video camera shows that the dim spot results from the surface discharges (SDs). The surface discharge induced by the volume discharge (VD) has the decisive effect on the formation of the dim spot. The experiment above plays an important role in studying the formation mechanism of the hexagonal super-lattice pattern with light spot and

Phototropin 1 and dim-blue light modulate the red light de-etiolation response.

PubMed

Wang, Yihai; M Folta, Kevin

2014-01-01

Light signals regulate seedling morphological changes during de-etiolation through the coordinated actions of multiple light-sensing pathways. Previously we have shown that red-light-induced hypocotyl growth inhibition can be reversed by addition of dim blue light through the action of phototropin 1 (phot1). Here we further examine the fluence-rate relationships of this blue light effect in short-term (hours) and long-term (days) hypocotyl growth assays. The red stem-growth inhibition and blue promotion is a low-fluence rate response, and blue light delays or attenuates both the red light and far-red light responses. These de-etiolation responses include blue light reversal of red or far-red induced apical hook opening. This response also requires phot1. Cryptochromes (cry1 and cry2) are activated by higher blue light fluence-rates and override phot1's influence on hypocotyl growth promotion. Exogenous application of auxin transport inhibitor naphthylphthalamic acid abolished the blue light stem growth promotion in both hypocotyl growth and hook opening. Results from the genetic tests of this blue light effect in auxin transporter mutants, as well as phytochrome kinase substrate mutants indicated that aux1 may play a role in blue light reversal of red light response. Together, the phot1-mediated adjustment of phytochrome-regulated photomorphogenic events is most robust in dim blue light conditions and is likely modulated by auxin transport through its transporters.

Phototropin 1 and dim-blue light modulate the red light de-etiolation response

PubMed Central

Wang, Yihai; M Folta, Kevin

2014-01-01

Light signals regulate seedling morphological changes during de-etiolation through the coordinated actions of multiple light-sensing pathways. Previously we have shown that red-light-induced hypocotyl growth inhibition can be reversed by addition of dim blue light through the action of phototropin 1 (phot1). Here we further examine the fluence-rate relationships of this blue light effect in short-term (hours) and long-term (days) hypocotyl growth assays. The red stem-growth inhibition and blue promotion is a low-fluence rate response, and blue light delays or attenuates both the red light and far-red light responses. These de-etiolation responses include blue light reversal of red or far-red induced apical hook opening. This response also requires phot1. Cryptochromes (cry1 and cry2) are activated by higher blue light fluence-rates and override phot1's influence on hypocotyl growth promotion. Exogenous application of auxin transport inhibitor naphthylphthalamic acid abolished the blue light stem growth promotion in both hypocotyl growth and hook opening. Results from the genetic tests of this blue light effect in auxin transporter mutants, as well as phytochrome kinase substrate mutants indicated that aux1 may play a role in blue light reversal of red light response. Together, the phot1-mediated adjustment of phytochrome-regulated photomorphogenic events is most robust in dim blue light conditions and is likely modulated by auxin transport through its transporters. PMID:25482790

Invasion of Ancestral Mammals into Dim-light Environments Inferred from Adaptive Evolution of the Phototransduction Genes

PubMed Central

Wu, Yonghua; Wang, Haifeng; Hadly, Elizabeth A.

2017-01-01

Nocturnality is a key evolutionary innovation of mammals that enables mammals to occupy relatively empty nocturnal niches. Invasion of ancestral mammals into nocturnality has long been inferred from the phylogenetic relationships of crown Mammalia, which is primarily nocturnal, and crown Reptilia, which is primarily diurnal, although molecular evidence for this is lacking. Here we used phylogenetic analyses of the vision genes involved in the phototransduction pathway to predict the diel activity patterns of ancestral mammals and reptiles. Our results demonstrated that the common ancestor of the extant Mammalia was dominated by positive selection for dim-light vision, supporting the predominate nocturnality of the ancestral mammals. Further analyses showed that the nocturnality of the ancestral mammals was probably derived from the predominate diurnality of the ancestral amniotes, which featured strong positive selection for bright-light vision. Like the ancestral amniotes, the common ancestor of the extant reptiles and various taxa in Squamata, one of the main competitors of the temporal niches of the ancestral mammals, were found to be predominate diurnality as well. Despite this relatively apparent temporal niche partitioning between ancestral mammals and the relevant reptiles, our results suggested partial overlap of their temporal niches during crepuscular periods. PMID:28425474

A neural network to improve dim-light vision? Dendritic fields of first-order interneurons in the nocturnal bee Megalopta genalis.

PubMed

Greiner, Birgit; Ribi, Willi A; Warrant, Eric J

2005-11-01

Using the combined Golgi-electron microscopy technique, we have determined the three-dimensional dendritic fields of the short visual fibres (svf 1-3) and first-order interneurons or L-fibres (L1-4) within the first optic ganglion (lamina) of the nocturnal bee Megalopta genalis. Serial cross sections have revealed that the svf type 2 branches into one adjacent neural unit (cartridge) in layer A, the most distal of the three lamina layers A, B and C. All L-fibres, except L1-a, exhibit wide lateral branching into several neighbouring cartridges. L1-b shows a dendritic field of seven cartridges in layers A and C, dendrites of L2 target 13 cartridges in layer A, L3 branches over a total of 12 cartridges in layer A and three in layer C and L4 has the largest dendritic field size of 18 cartridges in layer C. The number of cartridges reached by the respective L-fibres is distinctly greater in the nocturnal bee than in the worker honeybee and is larger than could be estimated from our previous Golgi-light microscopy study. The extreme dorso-ventrally oriented dendritic field of L4 in M. genalis may, in addition to its potential role in spatial summation, be involved in edge detection. Thus, we have shown that the amount of lateral spreading present in the lamina provides the anatomical basis for the required spatial summation. Theoretical and future physiological work should further elucidate the roles that this lateral spreading plays to improve dim-light vision in nocturnal insects.

Dim Light at Night Disrupts Molecular Circadian Rhythms and Affects Metabolism

PubMed Central

Fonken, Laura K.; Aubrecht, Taryn G.; Meléndez-Fernández, O. Hecmarie; Weil, Zachary M.; Nelson, Randy J.

2014-01-01

With the exception of high latitudes, life has evolved under bright days and dark nights. Most organisms have developed endogenously driven circadian rhythms which are synchronized to this daily light/dark cycle. In recent years, humans have shifted away from the naturally occurring solar light cycle in favor of artificial and sometimes irregular light schedules produced by electrical lighting. Exposure to unnatural light cycles is increasingly associated with obesity and metabolic syndrome; however the means by which environmental lighting alters metabolism are poorly understood. Thus, we exposed mice to nighttime light and investigated changes in the circadian system and body weight. Here we report that exposure to ecologically relevant levels of dim (5 lux) light at night attenuate core circadian clock rhythms in the SCN at both the gene and protein level. Moreover, circadian clock rhythms were perturbed in the liver by nighttime light exposure. Changes in the circadian clock were associated with temporal alterations in feeding behavior and increased weight gain. These results are significant because they provide mechanistic evidence for how mild changes in environmental lighting can alter circadian and metabolic function. PMID:23929553

Alternative Line Coding Scheme with Fixed Dimming for Visible Light Communication

NASA Astrophysics Data System (ADS)

Niaz, M. T.; Imdad, F.; Kim, H. S.

2017-01-01

An alternative line coding scheme called fixed-dimming on/off keying (FD-OOK) is proposed for visible-light communication (VLC). FD-OOK reduces the flickering caused by a VLC transmitter and can maintain a 50% dimming level. Simple encoder and decoder are proposed which generates codes where the number of bits representing one is same as the number of bits representing zero. By keeping the number of ones and zeros equal the change in the brightness of lighting may be minimized and kept constant at 50%, thereby reducing the flickering in VLC. The performance of FD-OOK is analysed with two parameters: the spectral efficiency and power requirement.

Mice exposed to dim light at night exaggerate inflammatory responses to lipopolysaccharide.

PubMed

Fonken, Laura K; Weil, Zachary M; Nelson, Randy J

2013-11-01

The mammalian circadian system regulates many physiological functions including inflammatory responses. Appropriately timed light information is essential for maintaining circadian organization. Over the past ∼120 years, urbanization and the widespread adoption of electric lights have dramatically altered lighting environments. Exposure to light at night (LAN) is pervasive in modern society and disrupts core circadian clock mechanisms. Because microglia are the resident macrophages in the brain and macrophages contain intrinsic circadian clocks, we hypothesized that chronic exposure to LAN would alter microglia cytokine expression and sickness behavior following LPS administration. Exposure to 4 weeks of dim LAN elevated inflammatory responses in mice. Mice exposed to dimly lit, as compared to dark, nights exaggerated changes in body temperature and elevated microglia pro-inflammatory cytokine expression following LPS administration. Furthermore, dLAN mice had a prolonged sickness response following the LPS challenge. Mice exposed to dark or dimly lit nights had comparable sickness behavior directly following the LPS injection; however, dLAN mice showed greater reductions in locomotor activity, increased anorectic behavior, and increased weight loss than mice maintained in dark nights 24h post-LPS injection. Overall, these data suggest that chronic exposure to even very low levels of light pollution may alter inflammatory responses. These results may have important implications for humans and other urban dwelling species that commonly experience nighttime light exposure. Copyright © 2013 Elsevier Inc. All rights reserved.

Transient anhedonia phenotype and altered circadian timing of behaviour during night-time dim light exposure in Per3-/- mice, but not wildtype mice.

PubMed

Martynhak, Bruno Jacson; Hogben, Alexandra L; Zanos, Panos; Georgiou, Polymnia; Andreatini, Roberto; Kitchen, Ian; Archer, Simon N; von Schantz, Malcolm; Bailey, Alexis; van der Veen, Daan R

2017-01-10

Industrialisation greatly increased human night-time exposure to artificial light, which in animal models is a known cause of depressive phenotypes. Whilst many of these phenotypes are 'direct' effects of light on affect, an 'indirect' pathway via altered sleep-wake timing has been suggested. We have previously shown that the Period3 gene, which forms part of the biological clock, is associated with altered sleep-wake patterns in response to light. Here, we show that both wild-type and Per3 -/- mice showed elevated levels of circulating corticosterone and increased hippocampal Bdnf expression after 3 weeks of exposure to dim light at night, but only mice deficient for the PERIOD3 protein (Per3 -/- ) exhibited a transient anhedonia-like phenotype, observed as reduced sucrose preference, in weeks 2-3 of dim light at night, whereas WT mice did not. Per3 -/- mice also exhibited a significantly smaller delay in behavioural timing than WT mice during weeks 1, 2 and 4 of dim light at night exposure. When treated with imipramine, neither Per3 -/- nor WT mice exhibited an anhedonia-like phenotype, and neither genotypes exhibited a delay in behavioural timing in responses to dLAN. While the association between both Per3 -/- phenotypes remains unclear, both are alleviated by imipramine treatment during dim night-time light.

Subjective quality of video sequences rendered on LCD with local backlight dimming at different lighting conditions

NASA Astrophysics Data System (ADS)

Mantel, Claire; Korhonen, Jari; Pedersen, Jesper M.; Bech, Søren; Andersen, Jakob Dahl; Forchhammer, Søren

2015-01-01

This paper focuses on the influence of ambient light on the perceived quality of videos displayed on Liquid Crystal Display (LCD) with local backlight dimming. A subjective test assessing the quality of videos with two backlight dimming methods and three lighting conditions, i.e. no light, low light level (5 lux) and higher light level (60 lux) was organized to collect subjective data. Results show that participants prefer the method exploiting local dimming possibilities to the conventional full backlight but that this preference varies depending on the ambient light level. The clear preference for one method at the low light conditions decreases at the high ambient light, confirming that the ambient light significantly attenuates the perception of the leakage defect (light leaking through dark pixels). Results are also highly dependent on the content of the sequence, which can modulate the effect of the ambient light from having an important influence on the quality grades to no influence at all.

Dim nighttime illumination interacts with parametric effects of bright light to increase the stability of circadian rhythm bifurcation in hamsters.

PubMed

Evans, Jennifer A; Elliott, Jeffrey A; Gorman, Michael R

2011-07-01

The endogenous circadian pacemaker of mammals is synchronized to the environmental day by the ambient cycle of relative light and dark. The present studies assessed the actions of light in a novel circadian entrainment paradigm where activity rhythms are bifurcated following exposure to a 24-h light:dark:light:dark (LDLD) cycle. Bifurcated entrainment under LDLD reflects the temporal dissociation of component oscillators that comprise the circadian system and is facilitated when daily scotophases are dimly lit rather than completely dark. Although bifurcation can be stably maintained in LDLD, it is quickly reversed under constant conditions. Here the authors examine whether dim scotophase illumination acts to maintain bifurcated entrainment under LDLD through potential interactions with the parametric actions of bright light during the two daily photophases. In three experiments, wheel-running rhythms of Syrian hamsters were bifurcated under LDLD with dimly lit scotophases, and after several weeks, dim scotophase illumination was either retained or extinguished. Additionally, "full" and "skeleton" photophases were employed under LDLD cycles with dimly lit or completely dark scotophases to distinguish parametric from nonparametric effects of bright light. Rhythm bifurcation was more stable in full versus skeleton LDLD cycles. Dim light facilitated the maintenance of bifurcated entrainment under full LDLD cycles but did not prevent the loss of rhythm bifurcation in skeleton LDLD cycles. These studies indicate that parametric actions of bright light maintain the bifurcated entrainment state; that dim scotophase illumination increases the stability of the bifurcated state; and that dim light interacts with the parametric effects of bright light to increase the stability of rhythm bifurcation under full LDLD cycles. A further understanding of the novel actions of dim light may lead to new strategies for understanding, preventing, and treating chronobiological

Nighttime dim light exposure alters the responses of the circadian system.

PubMed

Shuboni, D; Yan, L

2010-11-10

The daily light dark cycle is the most salient entraining factor for the circadian system. However, in modern society, darkness at night is vanishing as light pollution steadily increases. The impact of brighter nights on wild life ecology and human physiology is just now being recognized. In the present study, we tested the possible detrimental effects of dim light exposure on the regulation of circadian rhythms, using CD1 mice housed in light/dim light (LdimL, 300 lux:20 lux) or light/dark (LD, 300 lux:1 lux) conditions. We first examined the expression of clock genes in the suprachiasmatic nucleus (SCN), the locus of the principal brain clock, in the animals of the LD and LdimL groups. Under the entrained condition, there was no difference in PER1 peak expression between the two groups, but at the trough of the PER 1 rhythm, there was an increase in PER1 in the LdimL group, indicating a decrease in the amplitude of the PER1 rhythm. After a brief light exposure (30 min, 300 lux) at night, the light-induced expression of mPer1 and mPer2 genes was attenuated in the SCN of LdimL group. Next, we examined the behavioral rhythms by monitoring wheel-running activity to determine whether the altered responses in the SCN of LdimL group have behavioral consequence. Compared to the LD controls, the LdimL group showed increased daytime activity. After being released into constant darkness, the LdimL group displayed shorter free-running periods. Furthermore, following the light exposure, the phase shifting responses were smaller in the LdimL group. The results indicate that nighttime dim light exposure can cause functional changes of the circadian system, and suggest that altered circadian function could be one of the mechanisms underlying the adverse effects of light pollution on wild life ecology and human physiology. Copyright © 2010 IBRO. Published by Elsevier Ltd. All rights reserved.

Time-dependent effects of dim light at night on re-entrainment and masking of hamster activity rhythms.

PubMed

Frank, David W; Evans, Jennifer A; Gorman, Michael R

2010-04-01

Bright light has been established as the most ubiquitous environmental cue that entrains circadian timing systems under natural conditions. Light equivalent in intensity to moonlight (<1 lux), however, also strongly modulates circadian function in a number of entrainment paradigms. For example, compared to completely dark nights, dim nighttime illumination accelerated re-entrainment of hamster activity rhythms to 4-hour phase advances and delays of an otherwise standard laboratory photocycle. The purpose of this study was to determine if a sensitive period existed in the night during which dim illumination had a robust influence on speed of re-entrainment. Male Siberian hamsters were either exposed to dim light throughout the night, for half of the night, or not at all. Compared to dark nights, dim illumination throughout the entire night decreased by 29% the time for the midpoint of the active phase to re-entrain to a 4-hour phase advance and by 26% for a 4-hour delay. Acceleration of advances and delays were also achieved with 5 hours of dim light per night, but effects depended on whether dim light was present in the first half, second half, or first and last quarters of the night. Both during phase shifting and steady-state entrainment, partially lit nights also produced strong positive and negative masking effects, as well as entrainment aftereffects in constant darkness. Thus, even in the presence of a strong zeitgeber, light that might be encountered under a natural nighttime sky potently modulates the circadian timing system of hamsters.

Effect of evening exposure to bright or dim light after daytime bright light on absorption of dietary carbohydrates the following morning.

PubMed

Hirota, Naoko; Sone, Yoshiaki; Tokura, Hiromi

2010-01-01

We had previously reported on the effect of exposure to light on the human digestive system: daytime bright light exposure has a positive effect, whereas, evening bright light exposure has a negative effect on the efficiency of dietary carbohydrate absorption from the evening meal. These results prompted us to examine whether the light intensity to which subjects are exposed in the evening affects the efficiency of dietary carbohydrate absorption the following morning. In this study, subjects were exposed to either 50 lux (dim light conditions) or 2,000 lux (bright light conditions) in the evening for 9 h (from 15:00 to 24:00) after staying under bright light in the daytime (under 2,000 lux from 07:00 to 15:00). We measured unabsorbed dietary carbohydrates using the breath-hydrogen test the morning after exposure to either bright light or dim light the previous evening. Results showed that there was no significant difference between the two conditions in the amount of breath hydrogen. This indicates that evening exposure to bright or dim light after bright light exposure in the daytime has no varying effect on digestion or absorption of dietary carbohydrates in the following morning's breakfast.

Variation in rhodopsin kinase expression alters the dim flash response shut off and the light adaptation in rod photoreceptors.

PubMed

Sakurai, Keisuke; Young, Joyce E; Kefalov, Vladimir J; Khani, Shahrokh C

2011-08-29

Rod photoreceptors are exquisitely sensitive light detectors that function in dim light. The timely inactivation of their light responses is critical for the ability of rods to reliably detect and count photons. A key step in the inactivation of the rod transduction is the phosphorylation of the rod visual pigment, rhodopsin, catalyzed by G-protein-dependent receptor kinase 1 (GRK1). Absence of GRK1 greatly prolongs the photoreceptors' light response and enhances their susceptibility to degeneration. This study examined the light responses from mouse rods expressing various levels of GRK1 to evaluate how their function is modulated by rhodopsin inactivation. Transretinal and single-cell rod electrophysiological recordings were obtained from several strains of mice expressing GRK1 at 0.3- to 3-fold the wild-type levels. The effect of GRK1 expression level on the function of mouse rods was examined in darkness and during background adaptation. Altering the expression of GRK1 from 0.3- to 3-fold that in wild-type rods had little effect on the single photon response amplitude. Notably, increasing the expression level of GRK1 accelerated the dim flash response shut off but had no effect on the saturated response shut off. Additionally, GRK1 excess abolished the acceleration of saturated responses shut off during light adaptation. These results demonstrate that rhodopsin inactivation can modulate the kinetics of recovery from dim light stimulation. More importantly, the ratio of rhodopsin kinase to its modulator recoverin appears critical for the proper adaptation of rods and the acceleration of their response shut off in background light.

Dimming-discrete-multi-tone (DMT) for simultaneous color control and high speed visible light communication.

PubMed

Sung, Jiun-Yu; Chow, Chi-Wai; Yeh, Chien-Hung

2014-04-07

Visible light communication (VLC) using LEDs has attracted significant attention recently for the future secure, license-free and electromagnetic-interference (EMI)-free optical wireless communication. Dimming technique in LED lamp is advantageous for energy efficiency. Color control can be performed in the red-green-blue (RGB) LEDs by using dimming technique. It is highly desirable to employ dimming technique to provide simultaneous color and dimming control and high speed VLC. Here, we proposed and demonstrated a LED dimming control using dimming-discrete-multi-tone (DMT) modulation. High speed DMT-based VLC with simultaneous color and dimming control is demonstrated for the first time to the best of our knowledge. Demonstration and analyses for several modulation conditions and transmission distances are performed, for instance, demonstrating the data rate of 103.5 Mb/s (using RGB LED) with fast Fourier transform (FFT) size of 512.

Adaptations for vision in dim light: impulse responses and bumps in nocturnal spider photoreceptor cells (Cupiennius salei Keys).

PubMed

Pirhofer-Walzl, Karin; Warrant, Eric; Barth, Friedrich G

2007-10-01

The photoreceptor cells of the nocturnal spider Cupiennius salei were investigated by intracellular electrophysiology. (1) The responses of photoreceptor cells of posterior median (PM) and anterior median (AM) eyes to short (2 ms) light pulses showed long integration times in the dark-adapted and shorter integration times in the light-adapted state. (2) At very low light intensities, the photoreceptors responded to single photons with discrete potentials, called bumps, of high amplitude (2-20 mV). When measured in profoundly dark-adapted photoreceptor cells of the PM eyes these bumps showed an integration time of 128 +/- 35 ms (n = 7) whereas in dark-adapted photoreceptor cells of AM eyes the integration time was 84 +/- 13 ms (n = 8), indicating that the AM eyes are intrinsically faster than the PM eyes. (3) Long integration times, which improve visual reliability in dim light, and large responses to single photons in the dark-adapted state, contribute to a high visual sensitivity in Cupiennius at night. This conclusion is underlined by a calculation of sensitivity that accounts for both anatomical and physiological characteristics of the eye.

Experimental investigation of analog and digital dimming techniques on photometric performance of an indoor Visible Light Communication (VLC) system

NASA Astrophysics Data System (ADS)

Zafar, Fahad; Kalavally, Vineetha; Bakaul, Masuduzzaman; Parthiban, R.

2015-09-01

For making commercial implementation of light emitting diode (LED) based visible light communication (VLC) systems feasible, it is necessary to incorporate it with dimming schemes which will provide energy savings, moods and increase the aesthetic value of the places using this technology. There are two general methods which are used to dim LEDs commonly categorized as analog and digital dimming. Incorporating fast data transmission with these techniques is a key challenge in VLC. In this paper, digital and analog dimming for a 10 Mb/s non return to zero on-off keying (NRZ-OOK) based VLC system is experimentally investigated considering both photometric and communicative parameters. A spectrophotometer was used for photometric analysis and a line of sight (LOS) configuration in the presence of ambient light was used for analyzing communication parameters. Based on the experimental results, it was determined that digital dimming scheme is preferable for use in indoor VLC systems requiring high dimming precision and data transmission at lower brightness levels. On the other hand, analog dimming scheme is a cost effective solution for high speed systems where dimming precision is insignificant.

Transient anhedonia phenotype and altered circadian timing of behaviour during night-time dim light exposure in Per3−/− mice, but not wildtype mice

PubMed Central

Martynhak, Bruno Jacson; Hogben, Alexandra L.; Zanos, Panos; Georgiou, Polymnia; Andreatini, Roberto; Kitchen, Ian; Archer, Simon N.; von Schantz, Malcolm; Bailey, Alexis; van der Veen, Daan R.

2017-01-01

Industrialisation greatly increased human night-time exposure to artificial light, which in animal models is a known cause of depressive phenotypes. Whilst many of these phenotypes are ‘direct’ effects of light on affect, an ‘indirect’ pathway via altered sleep-wake timing has been suggested. We have previously shown that the Period3 gene, which forms part of the biological clock, is associated with altered sleep-wake patterns in response to light. Here, we show that both wild-type and Per3−/− mice showed elevated levels of circulating corticosterone and increased hippocampal Bdnf expression after 3 weeks of exposure to dim light at night, but only mice deficient for the PERIOD3 protein (Per3−/−) exhibited a transient anhedonia-like phenotype, observed as reduced sucrose preference, in weeks 2–3 of dim light at night, whereas WT mice did not. Per3−/− mice also exhibited a significantly smaller delay in behavioural timing than WT mice during weeks 1, 2 and 4 of dim light at night exposure. When treated with imipramine, neither Per3−/− nor WT mice exhibited an anhedonia-like phenotype, and neither genotypes exhibited a delay in behavioural timing in responses to dLAN. While the association between both Per3−/− phenotypes remains unclear, both are alleviated by imipramine treatment during dim night-time light. PMID:28071711

Effects of forward and backward transitions in light intensities in tau-illuminance curves of the rat motor activity rhythm under constant dim light.

PubMed

Cambras, Trinitat; Díez-Noguera, Antoni

2012-07-01

Circadian rhythms are strongly influenced by light intensity, the effects of which may persist beyond the duration of light exposure (aftereffects). Here, the authors constructed period-illuminance curves for the motor activity circadian rhythm of male and female rats by recording the effects of a series of small upward and downward steps in light intensity (illuminance ranging between .01 lux of dim red light and 1 lux of white light) on their activity. In all cases, stepwise changes were made in five logarithmic steps (irradiance: dim red light: .692 µW/cm(2) and white light: .006, .016, .044, .12, and .315 µW/cm(2), corresponding, respectively, to .02, .05, .14, .13, and 1 lux measured at cage level), with changes in intensity every 2 wks. One group of rats (DLD) started in dim red light, moved up to 1 lux white light, and then back down to the original light intensity. Another group (LDL) started at 1 lux, moved down to .01 lux, and then back up to the original intensity. Motor activity data were recorded throughout the experiment and tau values, the percentage of variance explained by the rhythm, and the mean motor activity for each stage and group were calculated. The results show differences in the dynamics of tau values between the DLD and LDL groups and between males and females. In the LDL group, the tau values of both males and females were dependent on light intensity, and were similar for the forward and backward transitions. In other words, no aftereffects were found, and no differences were detected between males and females. In the DLD group, however, differences were found between males and females. Males had a tau value of 24 h 20 min under dim red light, 25 h 40 min under 1 lux, and 24 h 50 min on return to dim red light. It is noticeable that the tau values of the backward branch of the illuminance curve contradicted classical predictions, since at .38 and .14 lux the tau values were shorter than those found under the same intensities after

Influences of diurnal bright or dim light exposure on urine volume in humans.

PubMed

Hyun, Ki-Ja; Nishimura, Shinya; Tokura, Hiromi

2006-03-01

We investigated with eight healthy females if 8 hr diurnal (0700 to 1500 h) bright rather than dim light (5,000 vs. 80 lx) influenced urine volume. Environmental illuminance was made identical at all other times besides 07:00 to 15:00 h. The participants spent time at strictly regulated schedules in a bioclimatic chamber (26 degrees C, relative humidity 60%) for 57 h. Blood was drawn (2 ml) just before lunch in order to calculate Creatinine clearance (Ccr). Urine volume was significantly higher during wakefulness and the 8-h sleep period with bright rather than dim light. Ccr was significantly higher after bright light. The results were discussed in terms of suppression of the sympathetic nerve system under the influence of diurnal bright light exposure. We also discussed these in terms of physiological polymorphisms.

A CDMA system implementation with dimming control for visible light communication

NASA Astrophysics Data System (ADS)

Chen, Danyang; Wang, Jianping; Jin, Jianli; Lu, Huimin; Feng, Lifang

2018-04-01

Visible light communication (VLC), using solid-state lightings to transmit information, has become a complement technology to wireless radio communication. As a realistic multiple access scheme for VLC system, code division multiple access (CDMA) has attracted more and more attentions in recent years. In this paper, we address and implement an improved CDMA scheme for VLC system. The simulation results reveal that the improved CDMA scheme not only supports multi-users' transmission but also maintains dimming value at about 50% and enhances the system efficiency. It can also realize the flexible dimming control by adjusting some parameters of system structure, which rarely affects the system BER performance. A real-time experimental VLC system with improved CDMA scheme is performed based on field programmable gate array (FPGA), reaching a good BER performance.

Variation in Rhodopsin Kinase Expression Alters the Dim Flash Response Shut Off and the Light Adaptation in Rod Photoreceptors

PubMed Central

Sakurai, Keisuke; Young, Joyce E.; Kefalov, Vladimir J.; Khani, Shahrokh C.

2011-01-01

Purpose. Rod photoreceptors are exquisitely sensitive light detectors that function in dim light. The timely inactivation of their light responses is critical for the ability of rods to reliably detect and count photons. A key step in the inactivation of the rod transduction is the phosphorylation of the rod visual pigment, rhodopsin, catalyzed by G-protein-dependent receptor kinase 1 (GRK1). Absence of GRK1 greatly prolongs the photoreceptors' light response and enhances their susceptibility to degeneration. This study examined the light responses from mouse rods expressing various levels of GRK1 to evaluate how their function is modulated by rhodopsin inactivation. Methods. Transretinal and single-cell rod electrophysiological recordings were obtained from several strains of mice expressing GRK1 at 0.3- to 3-fold the wild-type levels. The effect of GRK1 expression level on the function of mouse rods was examined in darkness and during background adaptation. Results. Altering the expression of GRK1 from 0.3- to 3-fold that in wild-type rods had little effect on the single photon response amplitude. Notably, increasing the expression level of GRK1 accelerated the dim flash response shut off but had no effect on the saturated response shut off. Additionally, GRK1 excess abolished the acceleration of saturated responses shut off during light adaptation. Conclusions. These results demonstrate that rhodopsin inactivation can modulate the kinetics of recovery from dim light stimulation. More importantly, the ratio of rhodopsin kinase to its modulator recoverin appears critical for the proper adaptation of rods and the acceleration of their response shut off in background light. PMID:21474765

Effect of evening exposure to dim or bright light on the digestion of carbohydrate in the supper meal.

PubMed

Hirota, Naoko; Sone, Yoshiaki; Tokura, Hiromi

2003-09-01

In a previous study we found that daytime exposure to bright as compared to dim light exerted a beneficial effect on the digestion of the evening meal. This finding prompted us to examine whether the digestion of the evening meal is also affected by evening light intensity. Subjects lived in light of 200 lux during the daytime (08:00-17:00 h) and took their evening meal at 17:00 h under 20 lux (evening dim-light condition: 17:00-02:00 h) or 2000 lux (evening bright-light condition: 17:00-02:00 h) until retiring at 02:00 h. Assessment of carbohydrate digestion of the evening meal was accomplished by a breath hydrogen test that is indicative of the malabsorption of dietary carbohydrate. Hydrogen excretion in the breath in the evening under the dim-light condition was significantly less than under the bright-light condition (p < 0.05). This finding is the opposite to that obtained in previous experiments in which subjects were exposed to the different intensities of light during the daytime, and indicates that the exposure to dim light in the evening exerts a better effect on carbohydrate digestion in the evening meal than does the exposure to bright light.

Home Circadian Phase Assessments with Measures of Compliance Yield Accurate Dim Light Melatonin Onsets

PubMed Central

Burgess, Helen J.; Wyatt, James K.; Park, Margaret; Fogg, Louis F.

2015-01-01

Study Objectives: There is a need for the accurate assessment of circadian phase outside of the clinic/laboratory, particularly with the gold standard dim light melatonin onset (DLMO). We tested a novel kit designed to assist in saliva sampling at home for later determination of the DLMO. The home kit includes objective measures of compliance to the requirements for dim light and half-hourly saliva sampling. Design: Participants were randomized to one of two 10-day protocols. Each protocol consisted of two back-to-back home and laboratory phase assessments in counterbalanced order, separated by a 5-day break. Setting: Laboratory or participants' homes. Participants: Thirty-five healthy adults, age 21–62 y. Interventions: N/A. Measurements and Results: Most participants received at least one 30-sec epoch of light > 50 lux during the home phase assessments (average light intensity 4.5 lux), but on average for < 9 min of the required 8.5 h. Most participants collected every saliva sample within 5 min of the scheduled time. Ninety-two percent of home DLMOs were not affected by light > 50 lux or sampling errors. There was no significant difference between the home and laboratory DLMOs (P > 0.05); on average the home DLMOs occurred 9.6 min before the laboratory DLMOs. The home DLMOs were highly correlated with the laboratory DLMOs (r = 0.91, P < 0.001). Conclusions: Participants were reasonably compliant to the home phase assessment procedures. The good agreement between the home and laboratory dim light melatonin onsets (DLMOs) demonstrates that including objective measures of light exposure and sample timing during home saliva sampling can lead to accurate home DLMOs. Clinical Trial Registration: Circadian Phase Assessments at Home, http://clinicaltrials.gov/show/NCT01487252, NCT01487252. Citation: Burgess HJ, Wyatt JK, Park M, Fogg LF. Home circadian phase assessments with measures of compliance yield accurate dim light melatonin onsets. SLEEP 2015;38(6):889–897

Bright to Dim Oscillatory Response of the Neurospora Circadian Oscillator

PubMed Central

Gooch, Van D.; Johnson, Alicia E.; Larrondo, Luis F.; Loros, Jennifer J.; Dunlap, Jay C.

2014-01-01

The fungus Neurospora crassa constitutes an important model system extensively used in chronobiology. Several studies have addressed how environmental cues, such as light, can reset or synchronize a circadian system. By means of an optimized firefly luciferase reporter gene and a controllable lighting system, we show that Neurospora can display molecular circadian rhythms in dim light when cultures receive bright light prior to entering dim light conditions. We refer to this behavior as the “bright to dim oscillatory response” (BDOR). The bright light treatment can be applied up to 76 h prior to dim exposure, and it can be as short as 15 min in duration. We have characterized this response in respect to the duration of the light pulse, the time of the light pulse before dim, the intensity of dim light, and the oscillation dynamics in dim light. Although the molecular mechanism that drives the BDOR remains obscure, these findings suggest that a long-term memory of bright light exists as part of the circadian molecular components. It is important to consider the ecological significance of such dim light responses in respect to how organisms naturally maintain their timing mechanism in moonlight. PMID:24492882

Bright to dim oscillatory response of the Neurospora circadian oscillator.

PubMed

Gooch, Van D; Johnson, Alicia E; Larrondo, Luis F; Loros, Jennifer J; Dunlap, Jay C

2014-02-01

The fungus Neurospora crassa constitutes an important model system extensively used in chronobiology. Several studies have addressed how environmental cues, such as light, can reset or synchronize a circadian system. By means of an optimized firefly luciferase reporter gene and a controllable lighting system, we show that Neurospora can display molecular circadian rhythms in dim light when cultures receive bright light prior to entering dim light conditions. We refer to this behavior as the "bright to dim oscillatory response" (BDOR). The bright light treatment can be applied up to 76 h prior to dim exposure, and it can be as short as 15 min in duration. We have characterized this response in respect to the duration of the light pulse, the time of the light pulse before dim, the intensity of dim light, and the oscillation dynamics in dim light. Although the molecular mechanism that drives the BDOR remains obscure, these findings suggest that a long-term memory of bright light exists as part of the circadian molecular components. It is important to consider the ecological significance of such dim light responses in respect to how organisms naturally maintain their timing mechanism in moonlight.

RdgB2 is required for dim-light input into intrinsically photosensitive retinal ganglion cells

PubMed Central

Walker, Marquis T.; Rupp, Alan; Elsaesser, Rebecca; Güler, Ali D.; Sheng, Wenlong; Weng, Shijun; Berson, David M.; Hattar, Samer; Montell, Craig

2015-01-01

A subset of retinal ganglion cells is intrinsically photosensitive (ipRGCs) and contributes directly to the pupillary light reflex and circadian photoentrainment under bright-light conditions. ipRGCs are also indirectly activated by light through cellular circuits initiated in rods and cones. A mammalian homologue (RdgB2) of a phosphoinositide transfer/exchange protein that functions in Drosophila phototransduction is expressed in the retinal ganglion cell layer. This raised the possibility that RdgB2 might function in the intrinsic light response in ipRGCs, which depends on a cascade reminiscent of Drosophila phototransduction. Here we found that under high light intensities, RdgB2−/− mutant mice showed normal pupillary light responses and circadian photoentrainment. Consistent with this behavioral phenotype, the intrinsic light responses of ipRGCs in RdgB2−/− were indistinguishable from wild-type. In contrast, under low-light conditions, RdgB2−/− mutants displayed defects in both circadian photoentrainment and the pupillary light response. The RdgB2 protein was not expressed in ipRGCs but was in GABAergic amacrine cells, which provided inhibitory feedback onto bipolar cells. We propose that RdgB2 is required in a cellular circuit that transduces light input from rods to bipolar cells that are coupled to GABAergic amacrine cells and ultimately to ipRGCs, thereby enabling ipRGCs to respond to dim light. PMID:26269578

Subjective time runs faster under the influence of bright rather than dim light conditions during the forenoon.

PubMed

Morita, Takeshi; Fukui, Tomoe; Morofushi, Masayo; Tokura, Hiromi

2007-05-16

The study investigated if 6 h morning bright light exposure, compared with dim light exposure, could influence time sense (range: 5-15 s). Eight women served as participants. The participant entered a bioclimatic chamber at 10:00 h on the day before the test day, where an ambient temperature and relative humidity were controlled at 25 degrees C and 60%RH. She sat quietly in a sofa in 50 lx until 22:00 h, retired at 22:00 h and then slept in total darkness. She rose at 07:00 h the following morning and again sat quietly in a sofa till 13:00 h, either in bright (2500 lx) or dim light (50 lx), the order of light intensities between the two occasions being randomized. The time-estimation test was performed from 13:00 to 13:10 h in 200 lx. The participant estimated the time that had elapsed between two buzzers, ranging over 5-15 s, and inputting the estimate into a computer. The test was carried out separately upon each individual. Results showed that the participants estimated higher durations of the given time intervals after previous exposure to 6 h of bright rather than dim light. The finding is discussed in terms of different load errors (difference between the actual core temperature and its thermoregulatory set-point) following 6-h exposure to bright or dim light in the morning.

Effect of dim and bright light exposure on some immunological parameters measured under thermal neutral conditions.

PubMed

Hyun, Ki-Ja; Kondo, Masayuki; Koh, Taichin; Tokura, Hiromi; Tamotsu, Satoshi; Oishi, Tadashi

2005-01-01

This study assesses the effects of ambient light conditions, under a thermoneutral environment, on selected immunological parameters of 7 healthy young women (aged 19 to 22 yrs). Subjects entered the bioclimatic chamber at 11: 00 h, controlled at 26 degrees C and 60% relative humidity, a "neutral climate". They lead a well-regulated life in the climatic chamber (pre-condition) while exposed to dim (200 lux) or, on the next day, bright (5000 lux) light between 06 : 00 to 12 : 00 h. Just before the end of each period of light exposure, a blood sample was taken for later immunological assay of white blood cell count (WBC), phagocytosis, interferon-gamma (IFN-gamma), interleukin-4 (IL-4), CD69 T cells (CD69), CD4+CD25+ T cells (CD4+CD25+), and transforming growth factor-beta 1 (TGF-beta1). The results, when compared with the pre-condition, were as follows: 1) CD69 and IFN-gamma increased during normal conditions without thermal stress under dim light; 2) WBC increased and IL-4 decreased under bright light; 3) as shown by the highly significant decrease of TGF-beta1, the immune system was activated under bright light; 4) phagocytosis tended to increase under bright light exposure; 5) CD69 and IFN-gamma were significantly higher, and CD4+CD25+ tended to decrease under bright light; 6) phagocytosis tended to be lower and TGF-beta1 significantly higher under dim light, indicating a decline of immune system function. Taken together, this preliminary single time-point sampling study infers that some parameters are activated (CD69) while others are attenuated (phagocytosis, TGF-beta1) according to the environmental light intensity, dim vs. bright, in women adhering to a standardized routine in the absence of thermal stress. These findings are discussed in terms of inhibition of the sympathetic and excitation of the parasympathetic nervous system under the influence of life-style regularity and daytime bright light exposure.

Bumblebees Perform Well-Controlled Landings in Dim Light.

PubMed

Reber, Therese; Dacke, Marie; Warrant, Eric; Baird, Emily

2016-01-01

To make a smooth touchdown when landing, an insect must be able to reliably control its approach speed as well as its body and leg position-behaviors that are thought to be regulated primarily by visual information. Bumblebees forage and land under a broad range of light intensities and while their behavior during the final moments of landing has been described in detail in bright light, little is known about how this is affected by decreasing light intensity. Here, we investigate this by characterizing the performance of bumblebees, B. terrestris, landing on a flat platform at two different orientations (horizontal and vertical) and at four different light intensities (ranging from 600 lx down to 19 lx). As light intensity decreased, the bees modified their body position and the distance at which they extended their legs, suggesting that the control of landing in these insects is visually mediated. Nevertheless, the effect of light intensity was small and the landings were still well controlled, even in the dimmest light. We suggest that the changes in landing behavior that occurred in dim light might represent adaptations that allow the bees to perform smooth landings across the broad range of light intensities at which they are active.

The Histone H3 Lysine 9 Methyltransferase DIM-5 Modifies Chromatin at frequency and Represses Light-Activated Gene Expression

PubMed Central

Ruesch, Catherine E.; Ramakrishnan, Mukund; Park, Jinhee; Li, Na; Chong, Hin S.; Zaman, Riasat; Joska, Tammy M.; Belden, William J.

2014-01-01

The transcriptional program controlling the circadian rhythm requires coordinated regulation of chromatin. Characterization of the chromodomain helicase DNA-binding enzyme CHD1 revealed DNA methylation in the promoter of the central clock gene frequency (frq) in Neurospora crassa. In this report, we show that the DNA methylation at frq is not only dependent on the DNA methyltransferase DIM-2 but also on the H3K9 methyltransferase DIM-5 and HP1. Histone H3 lysine 9 trimethylation (H3K9me3) occurs at frq and is most prominent 30 min after light-activated expression. Strains lacking dim-5 have an increase in light-induced transcription, and more White Collar-2 is found associated with the frq promoter. Consistent with the notion that DNA methylation assists in establishing the proper circadian phase, loss of H3K9 methylation results in a phase advance suggesting it delays the onset of frq expression. The dim-5 deletion strain displays an increase in circadian-regulated conidia formation on race tubes and there is a synthetic genetic interaction between dim-5 and ras-1bd. These results indicate DIM-5 has a regulatory role in muting circadian output. Overall, the data support a model where facultative heterochromatic at frq serves to establish the appropriate phase, mute the light response, and repress circadian output. PMID:25429045

The histone H3 lysine 9 methyltransferase DIM-5 modifies chromatin at frequency and represses light-activated gene expression.

PubMed

Ruesch, Catherine E; Ramakrishnan, Mukund; Park, Jinhee; Li, Na; Chong, Hin S; Zaman, Riasat; Joska, Tammy M; Belden, William J

2014-11-25

The transcriptional program controlling the circadian rhythm requires coordinated regulation of chromatin. Characterization of the chromodomain helicase DNA-binding enzyme CHD1 revealed DNA methylation in the promoter of the central clock gene frequency (frq) in Neurospora crassa. In this report, we show that the DNA methylation at frq is not only dependent on the DNA methyltransferase DIM-2 but also on the H3K9 methyltransferase DIM-5 and HP1. Histone H3 lysine 9 trimethylation (H3K9me3) occurs at frq and is most prominent 30 min after light-activated expression. Strains lacking dim-5 have an increase in light-induced transcription, and more White Collar-2 is found associated with the frq promoter. Consistent with the notion that DNA methylation assists in establishing the proper circadian phase, loss of H3K9 methylation results in a phase advance suggesting it delays the onset of frq expression. The dim-5 deletion strain displays an increase in circadian-regulated conidia formation on race tubes and there is a synthetic genetic interaction between dim-5 and ras-1(bd). These results indicate DIM-5 has a regulatory role in muting circadian output. Overall, the data support a model where facultative heterochromatic at frq serves to establish the appropriate phase, mute the light response, and repress circadian output. Copyright © 2015 Ruesch et al.

Modelling the KIC8462852 light curves: compatibility of the dips and secular dimming with an exocomet interpretation

NASA Astrophysics Data System (ADS)

Wyatt, M. C.; van Lieshout, R.; Kennedy, G. M.; Boyajian, T. S.

2018-02-01

This paper shows how the dips and secular dimming in the KIC8462852 light curve can originate in circumstellar material distributed around a single elliptical orbit (e.g. exocomets). The expected thermal emission and wavelength dependent dimming is derived for different orbital parameters and geometries, including dust that is optically thick to stellar radiation, and for a size distribution of dust with realistic optical properties. We first consider dust distributed evenly around the orbit, then show how to derive its uneven distribution from the optical light curve and to predict light curves at different wavelengths. The fractional luminosity of an even distribution is approximately the level of dimming times stellar radius divided by distance from the star at transit. Non-detection of dust thermal emission for KIC8462852 thus provides a lower limit on the transit distance to complement the 0.6 au upper limit imposed by 0.4 d dips. Unless the dust distribution is optically thick, the putative 16 per cent century-long secular dimming must have disappeared before the WISE 12 μm measurement in 2010, and subsequent 4.5 μm observations require transits at >0.05 au. However, self-absorption of thermal emission removes these constraints for opaque dust distributions. The passage of dust clumps through pericentre is predicted to cause infrared brightening lasting tens of days and dimming during transit, such that total flux received decreases at wavelengths <5 μm, but increases to potentially detectable levels at longer wavelengths. We suggest that lower dimming levels than seen for KIC8462852 are more common in the Galactic population and may be detected in future transit surveys.

Home Circadian Phase Assessments with Measures of Compliance Yield Accurate Dim Light Melatonin Onsets.

PubMed

Burgess, Helen J; Wyatt, James K; Park, Margaret; Fogg, Louis F

2015-06-01

There is a need for the accurate assessment of circadian phase outside of the clinic/laboratory, particularly with the gold standard dim light melatonin onset (DLMO). We tested a novel kit designed to assist in saliva sampling at home for later determination of the DLMO. The home kit includes objective measures of compliance to the requirements for dim light and half-hourly saliva sampling. Participants were randomized to one of two 10-day protocols. Each protocol consisted of two back-to-back home and laboratory phase assessments in counterbalanced order, separated by a 5-day break. Laboratory or participants' homes. Thirty-five healthy adults, age 21-62 y. N/A. Most participants received at least one 30-sec epoch of light > 50 lux during the home phase assessments (average light intensity 4.5 lux), but on average for < 9 min of the required 8.5 h. Most participants collected every saliva sample within 5 min of the scheduled time. Ninety-two percent of home DLMOs were not affected by light > 50 lux or sampling errors. There was no significant difference between the home and laboratory DLMOs (P > 0.05); on average the home DLMOs occurred 9.6 min before the laboratory DLMOs. The home DLMOs were highly correlated with the laboratory DLMOs (r = 0.91, P < 0.001). Participants were reasonably compliant to the home phase assessment procedures. The good agreement between the home and laboratory dim light melatonin onsets (DLMOs) demonstrates that including objective measures of light exposure and sample timing during home saliva sampling can lead to accurate home DLMOs. Circadian Phase Assessments at Home, http://clinicaltrials.gov/show/NCT01487252, NCT01487252. © 2015 Associated Professional Sleep Societies, LLC.

Potent circadian effects of dim illumination at night in hamsters.

PubMed

Gorman, Michael R; Evans, Jennifer A; Elliott, Jeffrey A

2006-01-01

Conventional wisdom holds that the circadian pacemaker of rodents and humans is minimally responsive to light of the intensity provided by dim moonlight and starlight. However, dim illumination (<0.005 lux) provided during the daily dark periods markedly alters entrainment in hamsters. Under dimly lit scotophases, compared to completely dark ones phases, the upper range of entrainment is increased by approximately 4 h, and re-entrainment is accelerated following transfer from long to short day lengths. Moreover, the incidence of bimodal entrainment to 24 h light:dark:light:dark cycles is increased fourfold. Notably, the nocturnal illumination inducing these pronounced effects is equivalent in photic energy to that of a 2 sec, 100 lux light pulse. These effects may be parsimoniously interpreted as an action of dim light on the phase relations between multiple oscillators comprising the circadian pacemaker. An action of dim light distinct from that underlying bright-light phase-resetting may promote more effective entrainment. Together, the present results refute the view that scotopic illumination is environmental "noise" and indicate that clock function is conspicuously altered by nighttime illumination like that experienced under dim moonlight and starlight. We interpret our results as evidence for a novel action of dim light on the coupling of multiple circadian oscillators.

Exercise attenuates the metabolic effects of dim light at night.

PubMed

Fonken, Laura K; Meléndez-Fernández, O Hecmarie; Weil, Zachary M; Nelson, Randy J

2014-01-30

Most organisms display circadian rhythms that coordinate complex physiological and behavioral processes to optimize energy acquisition, storage, and expenditure. Disruptions to the circadian system with environmental manipulations such as nighttime light exposure alter metabolic energy homeostasis. Exercise is known to strengthen circadian rhythms and to prevent weight gain. Therefore, we hypothesized providing mice a running wheel for voluntary exercise would buffer against the effects of light at night (LAN) on weight gain. Mice were maintained in either dark (LD) or dim (dLAN) nights and provided either a running wheel or a locked wheel. Mice exposed to dim, rather than dark, nights increased weight gain. Access to a functional running wheel prevented body mass gain in mice exposed to dLAN. Voluntary exercise appeared to limit weight gain independently of rescuing changes to the circadian system caused by dLAN; increases in daytime food intake induced by dLAN were not diminished by increased voluntary exercise. Furthermore, although all of the LD mice displayed a 24h rhythm in wheel running, nearly half (4 out of 9) of the dLAN mice did not display a dominant 24h rhythm in wheel running. These results indicate that voluntary exercise can prevent weight gain induced by dLAN without rescuing circadian rhythm disruptions. © 2013.

Bright versus dim ambient light affects subjective well-being but not serotonin-related biological factors.

PubMed

Stemer, Bettina; Melmer, Andreas; Fuchs, Dietmar; Ebenbichler, Christoph; Kemmler, Georg; Deisenhammer, Eberhard A

2015-10-30

Light falling on the retina is converted into an electrical signal which stimulates serotonin synthesis. Previous studies described an increase of plasma and CNS serotonin levels after bright light exposure. Ghrelin and leptin are peptide hormones which are involved in the regulation of hunger/satiety and are related to serotonin. Neopterin and kynurenine are immunological markers which are also linked to serotonin biosynthesis. In this study, 29 healthy male volunteers were exposed to bright (5000lx) and dim (50lx) light conditions for 120min in a cross-over manner. Subjective well-being and hunger as well as various serotonin associated plasma factors were assessed before and after light exposure. Subjective well-being showed a small increase under bright light and a small decrease under dim light, resulting in a significant interaction between light condition and time. Ghrelin concentrations increased significantly under both light conditions, but there was no interaction between light and time. Correspondingly, leptin decreased significantly under both light conditions. Hunger increased significantly with no light-time interaction. We also found a significant decrease of neopterin, tryptophan and tyrosine levels, but no interaction between light and time. In conclusion, ambient light was affecting subjective well-being rather than serotonin associated biological factors. Copyright © 2015. Published by Elsevier Ireland Ltd.

Exposure to dim light at night during early development increases adult anxiety-like responses.

PubMed

Borniger, Jeremy C; McHenry, Zachary D; Abi Salloum, Bachir A; Nelson, Randy J

2014-06-22

Early experiences produce effects that may persist throughout life. Therefore, to understand adult phenotype, it is important to investigate the role of early environmental stimuli in adult behavior and health. Artificial light at night (LAN) is an increasingly common phenomenon throughout the world. However, animals, including humans, evolved under dark night conditions. Many studies have revealed affective, immune, and metabolic alterations provoked by aberrant light exposure and subsequent circadian disruption. Pups are receptive to entraining cues from the mother and then light early during development, raising the possibility that the early life light environment may influence subsequent behavior. Thus, to investigate potential influences of early life exposure to LAN on adult phenotype, we exposed mice to dim (~5 lux; full spectrum white light) or dark (~0 lux) nights pre- and/or postnatally. After weaning at 3 weeks of age, all mice were maintained in dark nights until adulthood (9 weeks of age) when behavior was assessed. Mice exposed to dim light in early life increased anxiety-like behavior and fearful responses on the elevated plus maze and passive avoidance tests. These mice also displayed reduced growth rates, which ultimately normalized during adolescence. mRNA expression of brain derived neurotrophic factor (BDNF), a neurotrophin previously linked to early life environment and adult phenotype, was not altered in the prefrontal cortex or hippocampus by early life LAN exposure. Serum corticosterone concentrations were similar between groups at weaning, suggesting that early life LAN does not elicit a long-term physiologic stress response. Dim light exposure did not influence behavior on the open field, novel object, sucrose anhedonia, or forced swim tests. Our data highlight the potential deleterious consequences of low levels of light during early life to development and subsequent behavior. Whether these changes are due to altered maternal behavior

The molecular origin and evolution of dim-light vision in mammals.

PubMed

Bickelmann, Constanze; Morrow, James M; Du, Jing; Schott, Ryan K; van Hazel, Ilke; Lim, Steve; Müller, Johannes; Chang, Belinda S W

2015-11-01

The nocturnal origin of mammals is a longstanding hypothesis that is considered instrumental for the evolution of endothermy, a potential key innovation in this successful clade. This hypothesis is primarily based on indirect anatomical inference from fossils. Here, we reconstruct the evolutionary history of rhodopsin--the vertebrate visual pigment mediating the first step in phototransduction at low-light levels--via codon-based model tests for selection, combined with gene resurrection methods that allow for the study of ancient proteins. Rhodopsin coding sequences were reconstructed for three key nodes: Amniota, Mammalia, and Theria. When expressed in vitro, all sequences generated stable visual pigments with λMAX values similar to the well-studied bovine rhodopsin. Retinal release rates of mammalian and therian ancestral rhodopsins, measured via fluorescence spectroscopy, were significantly slower than those of the amniote ancestor, indicating altered molecular function possibly related to nocturnality. Positive selection along the therian branch suggests adaptive evolution in rhodopsin concurrent with therian ecological diversification events during the Mesozoic that allowed for an exploration of the environment at varying light levels. © 2015 The Author(s). Evolution © 2015 The Society for the Study of Evolution.

Light Vision Color

NASA Astrophysics Data System (ADS)

Valberg, Arne

2005-04-01

Light Vision Color takes a well-balanced, interdisciplinary approach to our most important sensory system. The book successfully combines basics in vision sciences with recent developments from different areas such as neuroscience, biophysics, sensory psychology and philosophy. Originally published in 1998 this edition has been extensively revised and updated to include new chapters on clinical problems and eye diseases, low vision rehabilitation and the basic molecular biology and genetics of colour vision. Takes a broad interdisciplinary approach combining basics in vision sciences with the most recent developments in the area Includes an extensive list of technical terms and explanations to encourage student understanding Successfully brings together the most important areas of the subject in to one volume

Insect photoreceptor adaptations to night vision.

PubMed

Honkanen, Anna; Immonen, Esa-Ville; Salmela, Iikka; Heimonen, Kyösti; Weckström, Matti

2017-04-05

Night vision is ultimately about extracting information from a noisy visual input. Several species of nocturnal insects exhibit complex visually guided behaviour in conditions where most animals are practically blind. The compound eyes of nocturnal insects produce strong responses to single photons and process them into meaningful neural signals, which are amplified by specialized neuroanatomical structures. While a lot is known about the light responses and the anatomical structures that promote pooling of responses to increase sensitivity, there is still a dearth of knowledge on the physiology of night vision. Retinal photoreceptors form the first bottleneck for the transfer of visual information. In this review, we cover the basics of what is known about physiological adaptations of insect photoreceptors for low-light vision. We will also discuss major enigmas of some of the functional properties of nocturnal photoreceptors, and describe recent advances in methodologies that may help to solve them and broaden the field of insect vision research to new model animals.This article is part of the themed issue 'Vision in dim light'. © 2017 The Author(s).

RdgB2 is required for dim-light input into intrinsically photosensitive retinal ganglion cells.

PubMed

Walker, Marquis T; Rupp, Alan; Elsaesser, Rebecca; Güler, Ali D; Sheng, Wenlong; Weng, Shijun; Berson, David M; Hattar, Samer; Montell, Craig

2015-10-15

A subset of retinal ganglion cells is intrinsically photosensitive (ipRGCs) and contributes directly to the pupillary light reflex and circadian photoentrainment under bright-light conditions. ipRGCs are also indirectly activated by light through cellular circuits initiated in rods and cones. A mammalian homologue (RdgB2) of a phosphoinositide transfer/exchange protein that functions in Drosophila phototransduction is expressed in the retinal ganglion cell layer. This raised the possibility that RdgB2 might function in the intrinsic light response in ipRGCs, which depends on a cascade reminiscent of Drosophila phototransduction. Here we found that under high light intensities, RdgB2(-/-) mutant mice showed normal pupillary light responses and circadian photoentrainment. Consistent with this behavioral phenotype, the intrinsic light responses of ipRGCs in RdgB2(-/-) were indistinguishable from wild-type. In contrast, under low-light conditions, RdgB2(-/-) mutants displayed defects in both circadian photoentrainment and the pupillary light response. The RdgB2 protein was not expressed in ipRGCs but was in GABAergic amacrine cells, which provided inhibitory feedback onto bipolar cells. We propose that RdgB2 is required in a cellular circuit that transduces light input from rods to bipolar cells that are coupled to GABAergic amacrine cells and ultimately to ipRGCs, thereby enabling ipRGCs to respond to dim light. © 2015 Walker et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

Neural mechanism for sensing fast motion in dim light.

PubMed

Li, Ran; Wang, Yi

2013-11-07

Luminance is a fundamental property of visual scenes. A population of neurons in primary visual cortex (V1) is sensitive to uniform luminance. In natural vision, however, the retinal image often changes rapidly. Consequently the luminance signals visual cells receive are transiently varying. How V1 neurons respond to such luminance changes is unknown. By applying large static uniform stimuli or grating stimuli altering at 25 Hz that resemble the rapid luminance changes in the environment, we show that approximately 40% V1 cells responded to rapid luminance changes of uniform stimuli. Most of them strongly preferred luminance decrements. Importantly, when tested with drifting gratings, the preferred speeds of these cells were significantly higher than cells responsive to static grating stimuli but not to uniform stimuli. This responsiveness can be accounted for by the preferences for low spatial frequencies and high temporal frequencies. These luminance-sensitive cells subserve the detection of fast motion under the conditions of dim illumination.

Fixation light hue bias revisited: implications for using adaptive optics to study color vision.

PubMed

Hofer, H J; Blaschke, J; Patolia, J; Koenig, D E

2012-03-01

Current vision science adaptive optics systems use near infrared wavefront sensor 'beacons' that appear as red spots in the visual field. Colored fixation targets are known to influence the perceived color of macroscopic visual stimuli (Jameson, D., & Hurvich, L. M. (1967). Fixation-light bias: An unwanted by-product of fixation control. Vision Research, 7, 805-809.), suggesting that the wavefront sensor beacon may also influence perceived color for stimuli displayed with adaptive optics. Despite its importance for proper interpretation of adaptive optics experiments on the fine scale interaction of the retinal mosaic and spatial and color vision, this potential bias has not yet been quantified or addressed. Here we measure the impact of the wavefront sensor beacon on color appearance for dim, monochromatic point sources in five subjects. The presence of the beacon altered color reports both when used as a fixation target as well as when displaced in the visual field with a chromatically neutral fixation target. This influence must be taken into account when interpreting previous experiments and new methods of adaptive correction should be used in future experiments using adaptive optics to study color. Copyright © 2012 Elsevier Ltd. All rights reserved.

A neuronal circuit for colour vision based on rod-cone opponency.

PubMed

Joesch, Maximilian; Meister, Markus

2016-04-14

In bright light, cone-photoreceptors are active and colour vision derives from a comparison of signals in cones with different visual pigments. This comparison begins in the retina, where certain retinal ganglion cells have 'colour-opponent' visual responses-excited by light of one colour and suppressed by another colour. In dim light, rod-photoreceptors are active, but colour vision is impossible because they all use the same visual pigment. Instead, the rod signals are thought to splice into retinal circuits at various points, in synergy with the cone signals. Here we report a new circuit for colour vision that challenges these expectations. A genetically identified type of mouse retinal ganglion cell called JAMB (J-RGC), was found to have colour-opponent responses, OFF to ultraviolet (UV) light and ON to green light. Although the mouse retina contains a green-sensitive cone, the ON response instead originates in rods. Rods and cones both contribute to the response over several decades of light intensity. Remarkably, the rod signal in this circuit is antagonistic to that from cones. For rodents, this UV-green channel may play a role in social communication, as suggested by spectral measurements from the environment. In the human retina, all of the components for this circuit exist as well, and its function can explain certain experiences of colour in dim lights, such as a 'blue shift' in twilight. The discovery of this genetically defined pathway will enable new targeted studies of colour processing in the brain.

The dim light melatonin onset following fixed and free sleep schedules.

PubMed

Burgess, Helen J; Eastman, Charmane I

2005-09-01

The time at which the dim light melatonin onset (DLMO) occurs can be used to ensure the correct timing of light and/or melatonin administration in order to produce desired circadian phase shifts. Sometimes however, measuring the DLMO is not feasible. Here we determined if the DLMO was best estimated from fixed sleep times (based on habitual sleep times) or free (ad libitum) sleep times. Young healthy sleepers on fixed (n=60) or free (n=60) sleep schedules slept at home for 6 days. Sleep times were recorded with sleep logs verified with wrist actigraphy. Half-hourly saliva samples were then collected during a dim light phase assessment and were later assayed to determine the DLMO. We found that the DLMO was more highly correlated with sleep times in the free sleepers than in the fixed sleepers (DLMO versus wake time, r=0.70 and r=0.44, both P<0.05). The regression equation between wake time and the DLMO in the free sleepers predicted the DLMO in an independent sample of free sleepers (n=23) to within 1.5 h of the actual DLMO in 96% of cases. These results indicate that the DLMO can be readily estimated in people whose sleep times are minimally affected by work, class and family commitments. Further work is necessary to determine if the DLMO can be accurately estimated in people with greater work and family responsibilities that affect their sleep times, perhaps by using weekend wake times, and if this method will apply to the elderly and patients with circadian rhythm disorders.

Dim light at night increases immune function in Nile grass rats, a diurnal rodent.

PubMed

Fonken, Laura K; Haim, Achikam; Nelson, Randy J

2012-02-01

With the widespread adoption of electrical lighting during the 20th century, human and nonhuman animals became exposed to high levels of light at night for the first time in evolutionary history. This divergence from the natural environment may have significant implications for certain ecological niches because of the important influence light exerts on the circadian system. For example, circadian disruption and nighttime light exposure are linked to changes in immune function. The majority of studies investigating the effects of light exposure and circadian disruption on the immune system use nocturnal rodents. In diurnal species, many hormones and immune parameters vary with secretion patterns 180° out of phase to those of nocturnal rodents. Thus, the authors investigated the effects of nighttime light exposure on immunocompetence in diurnal Nile grass rats (Arvicanthis niloticus). Rats were housed in either standard 14-h light (L):10-h dark (D) cycles with L ∼150 lux and D 0 lux or dim light at night (dLAN) cycles of LD 14:10 with L ∼150 lux and D 5 lux for 3 wks, then tested for plasma bactericidal capacity, as well as humoral and cell-mediated immune responses. Rats exposed to dLAN showed increased delayed-type hypersensitivity pinna swelling, which is consistent with enhanced cell-mediated immune function. dLAN rats similarly showed increased antibody production following inoculation with keyhole lymphocyte hemocyanin (KLH) and increased bactericidal capacity. Daytime corticosterone concentrations were elevated in grass rats exposed to nighttime dim light, which may have influenced immunological measures. Overall, these results indicate nighttime light affects immune parameters in a diurnal rodent.

Dim nighttime light impairs cognition and provokes depressive-like responses in a diurnal rodent.

PubMed

Fonken, Laura K; Kitsmiller, Emily; Smale, Laura; Nelson, Randy J

2012-08-01

Circadian disruption is a common by-product of modern life. Although jet lag and shift work are well-documented challenges to circadian organization, many more subtle environmental changes cause circadian disruption. For example, frequent fluctuations in the timing of the sleep/wake schedule, as well as exposure to nighttime lighting, likely affect the circadian system. Most studies of these effects have focused on nocturnal rodents, which are very different from diurnal species with respect to their patterns of light exposure and the effects that light can have on their activity. Thus, the authors investigated the effect of nighttime light on behavior and the brain of a diurnal rodent, the Nile grass rat. Following 3 weeks of exposure to standard light/dark (LD; 14:10 light [~150 lux] /dark [0 lux]) or dim light at night (dLAN; 14:10 light [~150 lux] /dim [5 lux]), rats underwent behavioral testing, and hippocampal neurons within CA1, CA3, and the dentate gyrus (DG) were examined. Three behavioral effects of dLAN were observed: (1) decreased preference for a sucrose solution, (2) increased latency to float in a forced swim test, and (3) impaired learning and memory in the Barnes maze. Light at night also reduced dendritic length in DG and basilar CA1 dendrites. Dendritic length in the DG positively correlated with sucrose consumption in the sucrose anhedonia task. Nighttime light exposure did not disrupt the pattern of circadian locomotor activity, and all grass rats maintained a diurnal activity pattern. Together, these data suggest that exposure to dLAN can alter affective responses and impair cognition in a diurnal animal.

An arousing, musically enhanced bird song stimulus mediates circadian rhythm phase advances in dim light.

PubMed

Goel, Namni

2006-09-01

A musically enhanced bird song stimulus presented in the early subjective night phase delays human circadian rhythms. This study determined the phase-shifting effects of the same stimulus in the early subjective day. Eleven subjects (ages 18-63 yr; mean +/- SD: 28.0 +/- 16.6 yr) completed two 4-day laboratory sessions in constant dim light (<20 lux). They received two consecutive presentations of either a 2-h musically enhanced bird song or control stimulus from 0600 to 0800 on the second and third mornings while awake. The 4-day sessions employing either the stimulus or control were counterbalanced. Core body temperature (CBT) was collected throughout the study, and salivary melatonin was obtained every 30 min from 1900 to 2330 on the baseline and poststimulus/postcontrol nights. Dim light melatonin onset and CBT minimum circadian phase before and after stimulus or control presentation was assessed. The musically enhanced bird song stimulus produced significantly larger phase advances of the circadian melatonin (mean +/- SD: 0.87 +/- 0.36 vs. 0.24 +/- 0.22 h) and CBT (1.08 +/- 0.50 vs. 0.43 +/- 0.37 h) rhythms than the control. The stimulus also decreased fatigue and total mood disturbance, suggesting arousing effects. This study shows that a musically enhanced bird song stimulus presented during the early subjective day phase advances circadian rhythms. However, it remains unclear whether the phase shifts are due directly to effects of the stimulus on the clock or are arousal- or dim light-mediated effects. This nonphotic stimulus mediates circadian resynchronization in either the phase advance or delay direction.

A design of LED adaptive dimming lighting system based on incremental PID controller

NASA Astrophysics Data System (ADS)

He, Xiangyan; Xiao, Zexin; He, Shaojia

2010-11-01

As a new generation energy-saving lighting source, LED is applied widely in various technology and industry fields. The requirement of its adaptive lighting technology is more and more rigorous, especially in the automatic on-line detecting system. In this paper, a closed loop feedback LED adaptive dimming lighting system based on incremental PID controller is designed, which consists of MEGA16 chip as a Micro-controller Unit (MCU), the ambient light sensor BH1750 chip with Inter-Integrated Circuit (I2C), and constant-current driving circuit. A given value of light intensity required for the on-line detecting environment need to be saved to the register of MCU. The optical intensity, detected by BH1750 chip in real time, is converted to digital signal by AD converter of the BH1750 chip, and then transmitted to MEGA16 chip through I2C serial bus. Since the variation law of light intensity in the on-line detecting environment is usually not easy to be established, incremental Proportional-Integral-Differential (PID) algorithm is applied in this system. Control variable obtained by the incremental PID determines duty cycle of Pulse-Width Modulation (PWM). Consequently, LED's forward current is adjusted by PWM, and the luminous intensity of the detection environment is stabilized by self-adaptation. The coefficients of incremental PID are obtained respectively after experiments. Compared with the traditional LED dimming system, it has advantages of anti-interference, simple construction, fast response, and high stability by the use of incremental PID algorithm and BH1750 chip with I2C serial bus. Therefore, it is suitable for the adaptive on-line detecting applications.

Contrast sensitivity to spatial gratings in moderate and dim light conditions in patients with diabetes in the absence of diabetic retinopathy.

PubMed

Safi, Sare; Rahimi, Anoushiravan; Raeesi, Afsaneh; Safi, Hamid; Aghazadeh Amiri, Mohammad; Malek, Mojtaba; Yaseri, Mehdi; Haeri, Mohammad; Middleton, Frank A; Solessio, Eduardo; Ahmadieh, Hamid

2017-01-01

To evaluate the ability of contrast sensitivity (CS) to discriminate loss of visual function in diabetic subjects with no clinical signs of retinopathy relative to that of normal subjects. In this prospective cross-sectional study, we measured CS in 46 diabetic subjects with a mean age of 48±6 years, a best-corrected visual acuity of 20/20 and no signs of diabetic retinopathy. The CS in these subjects was compared with CS measurements in 46 normal control subjects at four spatial frequencies (3, 6, 12, 18 cycles per degree) under moderate (500 lux) and dim (less than 2 lux) background light conditions. CS was approximately 0.16 log units lower in patients with diabetes relative to controls both in moderate and in dim background light conditions. Logistic regression classification and receiver operating characteristic curve analysis indicated that CS analysis using two light conditions was more accurate (0.78) overall compared with CS analysis using only a single illumination condition (accuracy values were 0.67 and 0.70 in moderate and dim light conditions, respectively). Our results showed that patients with diabetes without clinical signs of retinopathy exhibit a uniform loss in CS at all spatial frequencies tested. Measuring the loss in CS at two spatial frequencies (3 and 6 cycles per degree) and two light conditions (moderate and dim) is sufficiently robust to classify diabetic subjects with no retinopathy versus control subjects.

Contrast sensitivity to spatial gratings in moderate and dim light conditions in patients with diabetes in the absence of diabetic retinopathy

PubMed Central

Safi, Sare; Rahimi, Anoushiravan; Raeesi, Afsaneh; Safi, Hamid; Aghazadeh Amiri, Mohammad; Malek, Mojtaba; Yaseri, Mehdi; Haeri, Mohammad; Middleton, Frank A; Solessio, Eduardo; Ahmadieh, Hamid

2017-01-01

Objective To evaluate the ability of contrast sensitivity (CS) to discriminate loss of visual function in diabetic subjects with no clinical signs of retinopathy relative to that of normal subjects. Research design and methods In this prospective cross-sectional study, we measured CS in 46 diabetic subjects with a mean age of 48±6 years, a best-corrected visual acuity of 20/20 and no signs of diabetic retinopathy. The CS in these subjects was compared with CS measurements in 46 normal control subjects at four spatial frequencies (3, 6, 12, 18 cycles per degree) under moderate (500 lux) and dim (less than 2 lux) background light conditions. Results CS was approximately 0.16 log units lower in patients with diabetes relative to controls both in moderate and in dim background light conditions. Logistic regression classification and receiver operating characteristic curve analysis indicated that CS analysis using two light conditions was more accurate (0.78) overall compared with CS analysis using only a single illumination condition (accuracy values were 0.67 and 0.70 in moderate and dim light conditions, respectively). Conclusions Our results showed that patients with diabetes without clinical signs of retinopathy exhibit a uniform loss in CS at all spatial frequencies tested. Measuring the loss in CS at two spatial frequencies (3 and 6 cycles per degree) and two light conditions (moderate and dim) is sufficiently robust to classify diabetic subjects with no retinopathy versus control subjects. PMID:28878937

Insect photoreceptor adaptations to night vision

PubMed Central

Honkanen, Anna; Salmela, Iikka; Weckström, Matti

2017-01-01

Night vision is ultimately about extracting information from a noisy visual input. Several species of nocturnal insects exhibit complex visually guided behaviour in conditions where most animals are practically blind. The compound eyes of nocturnal insects produce strong responses to single photons and process them into meaningful neural signals, which are amplified by specialized neuroanatomical structures. While a lot is known about the light responses and the anatomical structures that promote pooling of responses to increase sensitivity, there is still a dearth of knowledge on the physiology of night vision. Retinal photoreceptors form the first bottleneck for the transfer of visual information. In this review, we cover the basics of what is known about physiological adaptations of insect photoreceptors for low-light vision. We will also discuss major enigmas of some of the functional properties of nocturnal photoreceptors, and describe recent advances in methodologies that may help to solve them and broaden the field of insect vision research to new model animals. This article is part of the themed issue ‘Vision in dim light’. PMID:28193821

The dim light melatonin onset following fixed and free sleep schedules

PubMed Central

Burgess, Helen J.; Eastman, Charmane I.

2013-01-01

Summary The time at which the dim light melatonin onset (DLMO) occurs can be used to ensure the correct timing of light and/or melatonin administration in order to produce desired circadian phase shifts. Sometimes however, measuring the DLMO is not feasible. Here we determined if the DLMO was best estimated from fixed sleep times (based on habitual sleep times) or free (ad libitum) sleep times. Young healthy sleepers on fixed (n = 60) or free (n = 60) sleep schedules slept at home for 6 days. Sleep times were recorded with sleep logs verified with wrist actigraphy. Half-hourly saliva samples were then collected during a dim light phase assessment and were later assayed to determine the DLMO. We found that the DLMO was more highly correlated with sleep times in the free sleepers than in the fixed sleepers (DLMO versus wake time, r = 0.70 and r = 0.44, both P < 0.05). The regression equation between wake time and the DLMO in the free sleepers predicted the DLMO in an independent sample of free sleepers (n = 23) to within 1.5 h of the actual DLMO in 96% of cases. These results indicate that the DLMO can be readily estimated in people whose sleep times are minimally affected by work, class and family commitments. Further work is necessary to determine if the DLMO can be accurately estimated in people with greater work and family responsibilities that affect their sleep times, perhaps by using weekend wake times, and if this method will apply to the elderly and patients with circadian rhythm disorders. PMID:16120097

Dim light at night prior to adolescence increases adult anxiety-like behaviors.

PubMed

Cissé, Yasmine M; Peng, Juan; Nelson, Randy J

2016-01-01

Dim light at night (dLAN) disrupts circadian organization and influences adult behavior. We examined early dLAN exposure on adult affective responses. Beginning 3 (juvenile) or 5 weeks (adolescent) of age, mice were maintained in standard light-dark cycles or exposed to nightly dLAN (5 lx) for 5 weeks, then anxiety-like and fear responses were assessed. Hypothalami were collected around the clock to assess core clock genes. Exposure to dLAN at either age increased anxiety-like responses in adults. Clock and Rev-ERB expression were altered by exposure to dLAN. In contrast to adults, dLAN exposure during early life increases anxiety and fear behavior.

Dim light at night provokes depression-like behaviors and reduces CA1 dendritic spine density in female hamsters.

PubMed

Bedrosian, Tracy A; Fonken, Laura K; Walton, James C; Haim, Abraham; Nelson, Randy J

2011-08-01

The prevalence of major depression has increased in recent decades; however, the underlying causes of this phenomenon remain unspecified. One environmental change that has coincided with elevated rates of depression is increased exposure to artificial light at night. Shift workers and others chronically exposed to light at night are at increased risk of mood disorders, suggesting that nighttime illumination may influence brain mechanisms mediating affect. We tested the hypothesis that exposure to dim light at night may impact affective responses and alter morphology of hippocampal neurons. Ovariectomized adult female Siberian hamsters (Phodopus sungorus) were housed for 8 weeks in either a light/dark cycle (LD) or a light/dim light cycle (DM), and then behavior was assayed. DM-hamsters displayed more depression-like responses in the forced swim and the sucrose anhedonia tests compared with LD-hamsters. Conversely, in the elevated plus maze DM-hamsters reduced anxiety-like behaviors. Brains from the same animals were processed using the Golgi-Cox method and hippocampal neurons within CA1, CA3, and the dentate gyrus were analyzed for morphological characteristics. In CA1, DM-hamsters significantly reduced dendritic spine density on both apical and basilar dendrites, an effect which was not mediated by baseline cortisol, as concentrations were equivalent between groups. These results demonstrate dim light at night is sufficient to reduce synaptic spine connections to CA1. Importantly, the present results suggest that night-time low level illumination, comparable to levels that are pervasive in North America and Europe, may contribute to the increasing prevalence of mood disorders. Copyright © 2011 Elsevier Ltd. All rights reserved.

Night Vision Manual for the Flight Surgeon

DTIC Science & Technology

1992-08-01

may cause night blindness are glaucoma, progressive cone/rod dystrophies (e.g., retinitis pigmentosa , Stargardt’s disease), drug toxicity (e.g...Alabama, July 1989. 38. Berson EL, Rabin AR, Mehaffey L. Advances in night vision twchnology: A pocketscope for patients with retinitis pigmentosa ... retinal sensitivity to dim light. Regeneration of the photopigments occurs during dark adaptation. The fully dark-adapted eye, in which photopigment

Vision in the dimmest habitats on earth.

PubMed

Warrant, Eric

2004-10-01

A very large proportion of the world's animal species are active in dim light, either under the cover of night or in the depths of the sea. The worlds they see can be dim and extended, with light reaching the eyes from all directions at once, or they can be composed of bright point sources, like the multitudes of stars seen in a clear night sky or the rare sparks of bioluminescence that are visible in the deep sea. The eye designs of nocturnal and deep-sea animals have evolved in response to these two very different types of habitats, being optimised for maximum sensitivity to extended scenes, or to point sources, or to both. After describing the many visual adaptations that have evolved across the animal kingdom for maximising sensitivity to extended and point-source scenes, I then use case studies from the recent literature to show how these adaptations have endowed nocturnal animals with excellent vision. Nocturnal animals can see colour and negotiate dimly illuminated obstacles during flight. They can also navigate using learned terrestrial landmarks, the constellations of stars or the dim pattern of polarised light formed around the moon. The conclusion from these studies is clear: nocturnal habitats are just as rich in visual details as diurnal habitats are, and nocturnal animals have evolved visual systems capable of exploiting them. The same is certainly true of deep-sea animals, as future research will no doubt reveal.

Dim-light sensitivity of cells in the awake cat's lateral geniculate and medial interlaminar nuclei: a correlation with behavior.

PubMed

Kang, Incheol; Malpeli, Joseph G

2009-08-01

Contrast thresholds of cells in the dorsal lateral geniculate (LGNd) and medial interlaminar (MIN) nuclei of awake cats were measured for scotopic and mesopic vision with drifting sine gratings (1/8, 2, and 4 cycles/deg [cpd]; 4-Hz temporal frequency). Thresholds for mean firing rate (F0) and temporally modulated responses (F1) were derived with receiver-operating-characteristic analyses and compared with behavioral measures recently reported by Kang and colleagues. Behavioral sensitivity was predicted by the neural responses of the most sensitive combinations of cell class and response mode: Y-cell F1 responses for 1/8 cpd, X-cell F1 responses for 2 cpd, and Y-cell F0 responses for 4 cpd. All previous estimates of neural scotopic increment thresholds in animal models fell between Weber's law (proportional to retinal illuminance) and the deVries-Rose law (proportional to the square root of illuminance). However, psychophysical experiments suggest that under appropriate conditions human scotopic vision follows the deVries-Rose law. If behavioral sensitivity is assumed to be determined by the most sensitive class of cells, this discrepancy is resolved. Under scotopic conditions, off-center Y cells were the most sensitive and these followed the deVries-Rose law fairly closely. MIN Y cells were, on average, 0.25 log units more sensitive than LGNd Y cells under scotopic conditions, supporting a previous proposal that the MIN is a specialization of the carnivore for dim-light vision. We conclude that both physiologically and behaviorally, cat and human scotopic vision are fundamentally similar, including adherence to the deVries-Rose law for detection of Gabor functions.

Dim light at night interferes with the development of the short-day phenotype and impairs cell-mediated immunity in Siberian hamsters (Phodopus sungorus).

PubMed

Aubrecht, Taryn G; Weil, Zachary M; Nelson, Randy J

2014-10-01

Winter is a challenging time to survive and breed outside of the tropics. Animals use day length (photoperiod) to regulate seasonally appropriate adaptations in anticipation of challenging winter conditions. The net result of these photoperiod-mediated adjustments is enhanced immune function and increased survival. Thus, the ability to discriminate day length information is critical for survival and reproduction in small animals. However, during the past century, urban and suburban development has rapidly expanded and filled the night sky with light from various sources, obscuring crucial light-dark signals, which alters physiological interpretation of day lengths. Furthermore, reduced space, increased proximity to people, and the presence of light at night may act as stressors for small animals. Whereas acute stressors typically enhance immune responses, chronic exposure to stressors often impairs immune responses. Therefore, we hypothesized that the combination of dim light at night and chronic stress interferes with enhanced cell-mediated immunity observed during short days. Siberian hamsters (Phodopus sungorus) were assigned to short or long days with dark nights (0 lux) or dim (5 lux) light at night for 10 weeks. Following 2 weeks of chronic restraint (6 hr/day), a model of chronic stress, delayed type hypersensitivity (DTH) responses were assessed. Both dim light at night and restraint reduced the DTH response. Dim light at night during long nights produced an intermediate short day phenotype. These results suggest the constant presence of light at night could negatively affect survival of photoperiodic rodents by disrupting the timing of breeding and immune responses. © 2014 Wiley Periodicals, Inc.

Dim light at night: physiological effects and ecological consequences for infectious disease.

PubMed

Kernbach, Meredith E; Hall, Richard J; Burkett-Cadena, Nathan; Unnasch, Thomas R; Martin, Lynn B

2018-06-23

Light pollution has emerged as a pervasive component of land development over the past century. Several detrimental impacts of this anthropogenic influence have been identified in night shift workers, laboratory rodents, and a plethora of wildlife species. Circadian, or daily, patterns are interrupted by the presence of light at night and have the capacity to alter rhythmic physiological or behavioral characteristics. Indeed, biorhythm disruption can lead to metabolic, reproductive, and immunological dysfunction depending on the intensity, timing, duration and wavelength of light exposure. Light pollution, in many forms and by many pathways, is thus apt to affect the nature of host-pathogen interactions. However, no research has yet investigated this possibility. The goal of this manuscript is to outline how dim light at night (dLAN), a relevant and common form of light pollution, may affect disease dynamics by interrupting circadian rhythms and regulation of immune responses as well as opportunities for host-parasite interactions and subsequent transmission risk including spillover into humans. We close by proposing some promising interventions including alternative lighting methods or vector control efforts.

Dim Light at Night Prior to Adolescence Increases Adult Anxiety-like Behaviors

PubMed Central

Cissé, Yasmine M.; Peng, Juan; Nelson, Randy J.

2017-01-01

Dim light at night (dLAN) disrupts circadian organization and influences adult behavior. We examined early dLAN exposure on adult affective responses. Beginning 3 (juvenile) or 5 weeks (adolescent) of age, mice were maintained in standard light-dark cycles or exposed to nightly dLAN (5 lux) for 5 weeks, then anxiety-like and fear responses were assessed. Hypothalami were collected around the clock to assess core clock genes. Exposure to dLAN at either age increased anxiety-like responses in adults. Clock and Rev-ERB expression were altered by exposure to dLAN. In contrast to adults, dLAN exposure during early life increases anxiety and fear behavior. PMID:27592634

Pre-flare coronal dimmings

NASA Astrophysics Data System (ADS)

Zhang, Q. M.; Su, Y. N.; Ji, H. S.

2017-02-01

Context. Coronal dimmings are regions of decreased extreme-ultravoilet (EUV) and/or X-ray (originally Skylab, then Yohkoh/SXT) intensities, which are often associated with flares and coronal mass ejections (CMEs). The large-scale impulsive dimmings have been thoroughly observed and investigated. The pre-flare dimmings before the flare impulsive phase, however, have rarely been studied in detail. Aims: We focus on the pre-flare coronal dimmings. We report our multiwavelength observations of the GOES X1.6 solar flare and the accompanying halo CME that was produced by the eruption of a sigmoidal magnetic flux rope (MFR) in NOAA active region (AR) 12158 on 2014 September 10. Methods: The eruption was observed by the Atmospheric Imaging Assembly (AIA) on board the Solar Dynamic Observatory (SDO). The photospheric line-of-sight magnetograms were observed by the Helioseismic and Magnetic Imager (HMI) on board SDO. The soft X-ray (SXR) fluxes were recorded by the GOES spacecraft. The halo CME was observed by the white-light coronagraphs of the Large Angle Spectroscopic Coronagraph (LASCO) on board SOHO. Results: About 96 min before the onset of the flare/CME, narrow pre-flare coronal dimmings appeared at the two ends of the twisted MFR. They extended very slowly, with their intensities decreasing with time, while their apparent widths (8-9 Mm) continued to be nearly constant. During the impulsive and decay phases of flare, typical fan-like twin dimmings appeared and expanded, with a much larger extent and lower intensities than the pre-flare dimmings. The percentage of the 171 Å intensity decrease reaches 40%. The pre-flare dimmings are most striking in 171, 193, and 211 Å with formation temperatures of 0.6-2.5 MK. The northern part of the pre-flare dimmings could also be recognized in 131 and 335 Å. Conclusions: To our knowledge, this is the first detailed study of pre-flare coronal dimmings; they can be explained by density depletion as a result of the gradual

Laboratory validation of an in-home method for assessing circadian phase using dim light melatonin onset (DLMO).

PubMed

Pullman, Rebecca E; Roepke, Stephanie E; Duffy, Jeanne F

2012-06-01

To determine whether an accurate circadian phase assessment could be obtained from saliva samples collected by patients in their home. Twenty-four individuals with a complaint of sleep initiation or sleep maintenance difficulty were studied for two evenings. Each participant received instructions for collecting eight hourly saliva samples in dim light at home. On the following evening they spent 9h in a laboratory room with controlled dim (<20 lux) light where hourly saliva samples were collected. Circadian phase of dim light melatonin onset (DLMO) was determined using both an absolute threshold (3 pg ml(-1)) and a relative threshold (two standard deviations above the mean of three baseline values). Neither threshold method worked well for one participant who was a "low-secretor". In four cases the participants' in-lab melatonin levels rose much earlier or were much higher than their at-home levels, and one participant appeared to take the at home samples out of order. Overall, the at-home and in-lab DLMO values were significantly correlated using both methods, and differed on average by 37 (± 19)min using the absolute threshold and by 54 (± 36)min using the relative threshold. The at-home assessment procedure was able to determine an accurate DLMO using an absolute threshold in 62.5% of the participants. Thus, an at-home procedure for assessing circadian phase could be practical for evaluating patients for circadian rhythm sleep disorders. Copyright © 2012 Elsevier B.V. All rights reserved.

Dim artificial light at night affects mating, reproductive output, and reactive oxygen species in Drosophila melanogaster.

PubMed

McLay, Lucy Katherine; Nagarajan-Radha, Venkatesh; Green, Mark Philip; Jones, Therésa Melanie

2018-05-07

Humans are lighting the night-time environment with ever increasing extent and intensity, resulting in a variety of negative ecological effects in individuals and populations. Effects of light at night on reproductive fitness traits are demonstrated across taxa however, the mechanisms underlying these effects are largely untested. One possible mechanism is that light at night may result in perturbed reactive oxygen species (ROS) and oxidative stress levels. Here, we reared Drosophila melanogaster under either dim (10 lx) light or no light (0 lx) at night for three generations and then compared mating and lifetime oviposition patterns. In a second experiment, we explored whether exposure to light at night treatments resulted in variation in ROS levels in the heads and ovaries of six, 23- and 36-day-old females. We demonstrate that dim light at night affects mating and reproductive output: 10 lx flies courted for longer prior to mating, and female oviposition patterns differed to 0 lx females. ROS levels were lower in the ovaries but not heads, of 10 lx compared with 0 lx females. We suggest that reduced ROS levels may reflect changes in ovarian physiology and cell signaling, which may be related to the differences observed in oviposition patterns. Taken together, our results indicate negative consequences for invertebrates under more stressful, urban, lit conditions and further investigation into the mechanisms driving these changes is warranted to manage invertebrate communities in a brighter future. © 2018 Wiley Periodicals, Inc.

Home Lighting Assessment for Clients With Low Vision

PubMed Central

Bhorade, Anjali; Gordon, Mae; Hollingsworth, Holly; Engsberg, Jack E.; Baum, M. Carolyn

2013-01-01

OBJECTIVE. The goal was to develop an objective, comprehensive, near-task home lighting assessment for older adults with low vision. METHOD. A home lighting assessment was developed and tested with older adults with low vision. Interrater and test–retest reliability studies were conducted. Clinical utility was assessed by occupational therapists with expertise in low vision rehabilitation. RESULTS. Interrater reliability was high (intraclass correlation coefficient [ICC] = .83–1.0). Test–retest reliability was moderate (ICC = .67). Responses to a Clinical Utility Feedback Form developed for this study indicated that the Home Environment Lighting Assessment (HELA) has strong clinical utility. CONCLUSION. The HELA provides a structured tool to describe the quantitative and qualitative aspects of home lighting environments where near tasks are performed and can be used to plan lighting interventions. The HELA has the potential to affect assessment and intervention practices of rehabilitation professionals in the area of low vision and improve near-task performance of people with low vision. PMID:24195901

The remarkable visual capacities of nocturnal insects: vision at the limits with small eyes and tiny brains

PubMed Central

2017-01-01

Nocturnal insects have evolved remarkable visual capacities, despite small eyes and tiny brains. They can see colour, control flight and land, react to faint movements in their environment, navigate using dim celestial cues and find their way home after a long and tortuous foraging trip using learned visual landmarks. These impressive visual abilities occur at light levels when only a trickle of photons are being absorbed by each photoreceptor, begging the question of how the visual system nonetheless generates the reliable signals needed to steer behaviour. In this review, I attempt to provide an answer to this question. Part of the answer lies in their compound eyes, which maximize light capture. Part lies in the slow responses and high gains of their photoreceptors, which improve the reliability of visual signals. And a very large part lies in the spatial and temporal summation of these signals in the optic lobe, a strategy that substantially enhances contrast sensitivity in dim light and allows nocturnal insects to see a brighter world, albeit a slower and coarser one. What is abundantly clear, however, is that during their evolution insects have overcome several serious potential visual limitations, endowing them with truly extraordinary night vision. This article is part of the themed issue ‘Vision in dim light’. PMID:28193808

The remarkable visual capacities of nocturnal insects: vision at the limits with small eyes and tiny brains.

PubMed

Warrant, Eric J

2017-04-05

Nocturnal insects have evolved remarkable visual capacities, despite small eyes and tiny brains. They can see colour, control flight and land, react to faint movements in their environment, navigate using dim celestial cues and find their way home after a long and tortuous foraging trip using learned visual landmarks. These impressive visual abilities occur at light levels when only a trickle of photons are being absorbed by each photoreceptor, begging the question of how the visual system nonetheless generates the reliable signals needed to steer behaviour. In this review, I attempt to provide an answer to this question. Part of the answer lies in their compound eyes, which maximize light capture. Part lies in the slow responses and high gains of their photoreceptors, which improve the reliability of visual signals. And a very large part lies in the spatial and temporal summation of these signals in the optic lobe, a strategy that substantially enhances contrast sensitivity in dim light and allows nocturnal insects to see a brighter world, albeit a slower and coarser one. What is abundantly clear, however, is that during their evolution insects have overcome several serious potential visual limitations, endowing them with truly extraordinary night vision.This article is part of the themed issue 'Vision in dim light'. © 2017 The Author(s).

Visual performance of four simultaneous-image multifocal contact lenses under dim and glare conditions.

PubMed

García-Lázaro, Santiago; Ferrer-Blasco, Teresa; Madrid-Costa, David; Albarrán-Diego, César; Montés-Micó, Robert

2015-01-01

To assess and compare the effects of four simultaneous-image multifocal contact lenses (SIMCLs), and those with distant-vision-only contact lenses on visual performance in early presbyopes, under dim conditions, including the effects of induced glare. In this double-masked crossover study design, 28 presbyopic subjects aged 40 to 46 years were included. All participants were fitted with the four different SIMCLs (Air Optix Aqua Multifocal [AOAM; Alcon], PureVision Multifocal [PM; Bausch & Lomb], Acuvue Oasys for Presbyopia [AOP; Johnson & Johnson Vision], and Biofinity Multifocal [BM; CooperVision]) and with monofocal contact lenses (Air Optix Aqua, Alcon). After 1 month of daily contact lens wearing, each subject's binocular distance visual acuity (BDVA) and binocular distance contrast sensitivity (BDCS) were measured using the Functional Visual Analyzer (Stereo Optical Co., Inc.) under mesopic conditions (3 candela [cd]/m) both with no glare and under the 2 levels of induced glare: 1.0 lux (glare 1) and 28 lux (glare 2). Among the SIMCLs, in terms of BDVA, AOAM and PM outperformed BM and AOP. All contact lenses performed better at level without glare, followed by Glare 1, and with the worst results obtained under glare 2. Binocular distance contrast sensitivity revealed statistically significant differences for 12 cycles per degree (cpd). Among the SIMCLs, post hoc multiple comparison testing revealed that AOAM and PM provided the best BDCS at the three luminance levels. In both cases, BDVA and BDCS at 12 cpd, monofocal contact lenses outperformed all SIMCL ones at all lighting conditions. Air Optix Aqua Multifocal and PM provided better visual performance than BM and AOP for distance vision with low addition and under dim conditions, but they all provide worse performance than monofocal contact lenses.

Moving in Dim Light: Behavioral and Visual Adaptations in Nocturnal Ants.

PubMed

Narendra, Ajay; Kamhi, J Frances; Ogawa, Yuri

2017-11-01

Visual navigation is a benchmark information processing task that can be used to identify the consequence of being active in dim-light environments. Visual navigational information that animals use during the day includes celestial cues such as the sun or the pattern of polarized skylight and terrestrial cues such as the entire panorama, canopy pattern, or significant salient features in the landscape. At night, some of these navigational cues are either unavailable or are significantly dimmer or less conspicuous than during the day. Even under these circumstances, animals navigate between locations of importance. Ants are a tractable system for studying navigation during day and night because the fine scale movement of individual animals can be recorded in high spatial and temporal detail. Ant species range from being strictly diurnal, crepuscular, and nocturnal. In addition, a number of species have the ability to change from a day- to a night-active lifestyle owing to environmental demands. Ants also offer an opportunity to identify the evolution of sensory structures for discrete temporal niches not only between species but also within a single species. Their unique caste system with an exclusive pedestrian mode of locomotion in workers and an exclusive life on the wing in males allows us to disentangle sensory adaptations that cater for different lifestyles. In this article, we review the visual navigational abilities of nocturnal ants and identify the optical and physiological adaptations they have evolved for being efficient visual navigators in dim-light. © The Author 2017. Published by Oxford University Press on behalf of the Society for Integrative and Comparative Biology. All rights reserved. For permissions please email: journals.permissions@oup.com.

Objective measures of sleep and dim light melatonin onset in adolescents and young adults with delayed sleep phase disorder compared to healthy controls.

PubMed

Saxvig, Ingvild W; Wilhelmsen-Langeland, Ane; Pallesen, Ståle; Vedaa, Oystein; Nordhus, Inger H; Sørensen, Eli; Bjorvatn, Bjørn

2013-08-01

Delayed sleep phase disorder is characterized by a delay in the timing of the major sleep period relative to conventional norms. The sleep period itself has traditionally been described as normal. Nevertheless, it is possible that sleep regulatory mechanism disturbances associated with the disorder may affect sleep duration and/or architecture. Polysomnographic data that may shed light on the issue are scarce. Hence, the aim of this study was to examine polysomnographic measures of sleep in adolescents and young adults with delayed sleep phase disorder, and to compare findings to that of healthy controls. A second aim was to estimate dim light melatonin onset as a marker of circadian rhythm and to investigate the phase angle relationship (time interval) between dim light melatonin onset and the sleep period. Data from 54 adolescents and young adults were analysed, 35 diagnosed with delayed sleep phase disorder and 19 healthy controls. Results show delayed timing of sleep in participants with delayed sleep phase disorder, but once sleep was initiated no group differences in sleep parameters were observed. Dim light melatonin onset was delayed in participants with delayed sleep phase disorder, but no difference in phase angle was observed between the groups. In conclusion, both sleep and dim light melatonin onset were delayed in participants with delayed sleep phase disorder. The sleep period appeared to occur at the same circadian phase in both groups, and once sleep was initiated no differences in sleep parameters were observed. © 2013 European Sleep Research Society.

Acute dim light at night increases body mass, alters metabolism, and shifts core body temperature circadian rhythms.

PubMed

Borniger, Jeremy C; Maurya, Santosh K; Periasamy, Muthu; Nelson, Randy J

2014-10-01

The circadian system is primarily entrained by the ambient light environment and is fundamentally linked to metabolism. Mounting evidence suggests a causal relationship among aberrant light exposure, shift work, and metabolic disease. Previous research has demonstrated deleterious metabolic phenotypes elicited by chronic (>4 weeks) exposure to dim light at night (DLAN) (∼ 5 lux). However, the metabolic effects of short-term (<2 weeks) exposure to DLAN are unspecified. We hypothesized that metabolic alterations would arise in response to just 2 weeks of DLAN. Specifically, we predicted that mice exposed to dim light would gain more body mass, alter whole body metabolism, and display altered body temperature (Tb) and activity rhythms compared to mice maintained in dark nights. Our data largely support these predictions; DLAN mice gained significantly more mass, reduced whole body energy expenditure, increased carbohydrate over fat oxidation, and altered temperature circadian rhythms. Importantly, these alterations occurred despite similar activity locomotor levels (and rhythms) and total food intake between groups. Peripheral clocks are potently entrained by body temperature rhythms, and the deregulation of body temperature we observed may contribute to metabolic problems due to "internal desynchrony" between the central circadian oscillator and temperature sensitive peripheral clocks. We conclude that even relatively short-term exposure to low levels of nighttime light can influence metabolism to increase mass gain.

A novel method for objective vision testing in canine models of inherited retinal disease.

PubMed

Gearhart, Patricia M; Gearhart, Chris C; Petersen-Jones, Simon M

2008-08-01

The use of canine models of retinal disease in the development of therapeutic strategies for inherited retinal disorders is a growing area of research. To evaluate accurately the success of potential vision-enhancing treatments, reliable methods for objectively assessing visual function in canine models is necessary. A simple vision-testing device was constructed that consisted of a junction box with four exit tunnels. Dogs were placed in the junction box and given one vision-based choice for exit. The first-choice tunnel and time to exit were recorded and analyzed. Two canine models of retinal disease with distinct molecular defects, a null mutation in the gene encoding the alpha subunit of rod cyclic GMP phosphodiesterase (PDE6A), and a null mutation in the gene encoding a retinal pigment epithelium-specific protein (RPE65) were tested and compared to those in unaffected dogs. With the use of bright light versus dim red light, the test differentiated between unaffected dogs and dogs affected with either mutation with a high degree of certainty. The white-light intensity series showed a significantly different performance between the unaffected and affected dogs. A significant difference in performance was detected between the dogs with each mutation. The results indicate that this novel canine vision-testing method is an accurate and sensitive means of distinguishing between unaffected dogs and dogs affected with two different forms of inherited retinal disease and should be useful as a means of assessing response to therapy in future studies.

Flicker Vision of Selected Light Sources

NASA Astrophysics Data System (ADS)

Otomański, Przemysław; Wiczyński, Grzegorz; Zając, Bartosz

2017-10-01

The results of the laboratory research concerning a dependence of flicker vision on voltage fluctuations are presented in the paper. The research was realized on a designed measuring stand, which included an examined light source, a voltage generator with amplitude modulation supplying the light source and a positioning system of the observer with respect to the observed surface. In this research, the following light sources were used: one incandescent lamp and four LED luminaires by different producers. The research results formulate a conclusion concerning the description of the influence of voltage fluctuations on flicker viewing for selected light sources. The research results indicate that LED luminaires are less susceptible to voltage fluctuations than incandescent bulbs and that flicker vision strongly depends on the type of LED source.

Assessing the Dim Light Melatonin Onset in Adults with Autism Spectrum Disorder and No Comorbid Intellectual Disability

ERIC Educational Resources Information Center

Baker, Emma K.; Richdale, Amanda L.; Hazi, Agnes; Prendergast, Luke A.

2017-01-01

This study assessed melatonin levels and the dim light melatonin onset (DLMO) in adults with Autism Spectrum Disorder (ASD) and also investigated the relationships between melatonin and objectively measured sleep parameters. Sixteen adults with ASD (ASD-Only), 12 adults with ASD medicated for comorbid diagnoses of anxiety and/or depression…

Inferred L/M cone opsin polymorphism of ancestral tarsiers sheds dim light on the origin of anthropoid primates.

PubMed

Melin, Amanda D; Matsushita, Yuka; Moritz, Gillian L; Dominy, Nathaniel J; Kawamura, Shoji

2013-05-22

Tarsiers are small nocturnal primates with a long history of fuelling debate on the origin and evolution of anthropoid primates. Recently, the discovery of M and L opsin genes in two sister species, Tarsius bancanus (Bornean tarsier) and Tarsius syrichta (Philippine tarsier), respectively, was interpreted as evidence of an ancestral long-to-middle (L/M) opsin polymorphism, which, in turn, suggested a diurnal or cathemeral (arrhythmic) activity pattern. This view is compatible with the hypothesis that stem tarsiers were diurnal; however, a reversion to nocturnality during the Middle Eocene, as evidenced by hyper-enlarged orbits, predates the divergence of T. bancanus and T. syrichta in the Late Miocene. Taken together, these findings suggest that some nocturnal tarsiers possessed high-acuity trichromatic vision, a concept that challenges prevailing views on the adaptive origins of the anthropoid visual system. It is, therefore, important to explore the plausibility and antiquity of trichromatic vision in the genus Tarsius. Here, we show that Sulawesi tarsiers (Tarsius tarsier), a phylogenetic out-group of Philippine and Bornean tarsiers, have an L opsin gene that is more similar to the L opsin gene of T. syrichta than to the M opsin gene of T. bancanus in non-synonymous nucleotide sequence. This result suggests that an L/M opsin polymorphism is the ancestral character state of crown tarsiers and raises the possibility that many hallmarks of the anthropoid visual system evolved under dim (mesopic) light conditions. This interpretation challenges the persistent nocturnal-diurnal dichotomy that has long informed debate on the origin of anthropoid primates.

Inferred L/M cone opsin polymorphism of ancestral tarsiers sheds dim light on the origin of anthropoid primates

PubMed Central

Melin, Amanda D.; Matsushita, Yuka; Moritz, Gillian L.; Dominy, Nathaniel J.; Kawamura, Shoji

2013-01-01

Tarsiers are small nocturnal primates with a long history of fuelling debate on the origin and evolution of anthropoid primates. Recently, the discovery of M and L opsin genes in two sister species, Tarsius bancanus (Bornean tarsier) and Tarsius syrichta (Philippine tarsier), respectively, was interpreted as evidence of an ancestral long-to-middle (L/M) opsin polymorphism, which, in turn, suggested a diurnal or cathemeral (arrhythmic) activity pattern. This view is compatible with the hypothesis that stem tarsiers were diurnal; however, a reversion to nocturnality during the Middle Eocene, as evidenced by hyper-enlarged orbits, predates the divergence of T. bancanus and T. syrichta in the Late Miocene. Taken together, these findings suggest that some nocturnal tarsiers possessed high-acuity trichromatic vision, a concept that challenges prevailing views on the adaptive origins of the anthropoid visual system. It is, therefore, important to explore the plausibility and antiquity of trichromatic vision in the genus Tarsius. Here, we show that Sulawesi tarsiers (Tarsius tarsier), a phylogenetic out-group of Philippine and Bornean tarsiers, have an L opsin gene that is more similar to the L opsin gene of T. syrichta than to the M opsin gene of T. bancanus in non-synonymous nucleotide sequence. This result suggests that an L/M opsin polymorphism is the ancestral character state of crown tarsiers and raises the possibility that many hallmarks of the anthropoid visual system evolved under dim (mesopic) light conditions. This interpretation challenges the persistent nocturnal–diurnal dichotomy that has long informed debate on the origin of anthropoid primates. PMID:23536597

Dim light at night does not disrupt timing or quality of sleep in mice.

PubMed

Borniger, Jeremy C; Weil, Zachary M; Zhang, Ning; Nelson, Randy J

2013-10-01

Artificial nighttime illumination has recently become commonplace throughout the world; however, in common with other animals, humans have not evolved in the ecological context of chronic light at night. With prevailing evidence linking the circadian, endocrine, immune, and metabolic systems, understanding these relationships is important to understanding the etiology and progression of several diseases. To eliminate the covariate of sleep disruption in light at night studies, researchers often use nocturnal animals. However, the assumption that light at night does not affect sleep in nocturnal animals remains unspecified. To test the effects of light at night on sleep, we maintained Swiss-Webster mice in standard light/dark (LD) or dim light at night (DLAN) conditions for 8-10 wks and then measured electroencephalogram (EEG) and electromyogram (EMG) biopotentials via wireless telemetry over the course of two consecutive days to determine differences in sleep timing and homeostasis. Results show no statistical differences in total percent time, number of episodes, maximum or average episode durations in wake, slow-wave sleep (SWS), or rapid eye movement (REM) sleep. No differences were evident in SWS delta power, an index of sleep drive, between groups. Mice kept in DLAN conditions showed a relative increase in REM sleep during the first few hours after the dark/light transition. Both groups displayed normal 24-h circadian rhythms as measured by voluntary running wheel activity. Groups did not differ in body mass, but a marked negative correlation of body mass with percent time spent awake and a positive correlation of body mass with time spent in SWS was evident. Elevated body mass was also associated with shorter maximum wake episode durations, indicating heavier animals had more trouble remaining in the wake vigilance state for extended periods of time. Body mass did not correlate with activity levels, nor did activity levels correlate with time spent in

EUV Dimmings as a Diagnostic of CMEs and Related Phenomena

NASA Technical Reports Server (NTRS)

Thompson, Barbara J.; Mays, M. Leila; Webb, David F.; West, Matthew J.

2012-01-01

Large-scale coronal EUV dimmings, developing on timescaJes of minutes to hours in association with a flare or filament eruption, are known to exhibit a high correlation with coronal mass ejections. While most observations indicate that the decrease in emission in a dimming is due, at least in part, to a density decrease, a complete understanding requires us to examine at least four mechanisms that have been observed to cause darkened regions in the corona: 1) mass loss, 2) cooling, 3) heating, and 4) absorption/obscuration. Recent advances in automatic detection, observations with improved cadence and resolution, multi-viewpoint imaging, and spectroscopic studies have continued to shed light on dimming formation, evolution, and recovery. However, there are still some outstanding questions, including 1) Why do some CMEs show dimming and some do not? 2) What determines the location of a dimming? 3) What determines the temporal evolution of a dimming? 4) How does the post-eruption dimming connect to the ICME? 5) What is the relationship between dimmings and other CME-associated phenomena? The talk will emphasize the different formation mechanisms of dimmings and their relationship to CMEs and CME-associated phenomena.

Chronic exposure to dim light at night suppresses immune responses in Siberian hamsters.

PubMed

Bedrosian, Tracy A; Fonken, Laura K; Walton, James C; Nelson, Randy J

2011-06-23

Species have been adapted to specific niches optimizing survival and reproduction; however, urbanization by humans has dramatically altered natural habitats. Artificial light at night (LAN), termed 'light pollution', is an often overlooked, yet increasing disruptor of habitats, which perturbs physiological processes that rely on precise light information. For example, LAN alters the timing of reproduction and activity in some species, which decreases the odds of successful breeding and increases the threat of predation for these individuals, leading to reduced fitness. LAN also suppresses immune function, an important proxy for survival. To investigate the impact of LAN in a species naive to light pollution in its native habitat, immune function was examined in Siberian hamsters derived from wild-caught stock. After four weeks exposure to dim LAN, immune responses to three different challenges were assessed: (i) delayed-type hypersensitivity (DTH), (ii) lipopolysaccharide-induced fever, and (iii) bactericide activity of blood. LAN suppressed DTH response and reduced bactericide activity of blood after lipopolysaccharide treatment, in addition to altering daily patterns of locomotor activity, suggesting that human encroachment on habitats via night-time lighting may inadvertently compromise immune function and ultimately fitness.

A sensor-less LED dimming system based on daylight harvesting with BIPV systems.

PubMed

Yoo, Seunghwan; Kim, Jonghun; Jang, Cheol-Yong; Jeong, Hakgeun

2014-01-13

Artificial lighting in office buildings typically requires 30% of the total energy consumption of the building, providing a substantial opportunity for energy savings. To reduce the energy consumed by indoor lighting, we propose a sensor-less light-emitting diode (LED) dimming system using daylight harvesting. In this study, we used light simulation software to quantify and visualize daylight, and analyzed the correlation between photovoltaic (PV) power generation and indoor illumination in an office with an integrated PV system. In addition, we calculated the distribution of daylight illumination into the office and dimming ratios for the individual control of LED lights. Also, we were able directly to use the electric power generated by PV system. As a result, power consumption for electric lighting was reduced by 40 - 70% depending on the season and the weather conditions. Thus, the dimming system proposed in this study can be used to control electric lighting to reduce energy use cost-effectively and simply.

Activation of AMP-Activated Protein Kinase by 3,3′-Diindolylmethane (DIM) Is Associated with Human Prostate Cancer Cell Death In Vitro and In Vivo

PubMed Central

Chen, Di; Banerjee, Sanjeev; Cui, Qiuzhi C.; Kong, Dejuan; Sarkar, Fazlul H.; Dou, Q. Ping

2012-01-01

There is a large body of scientific evidence suggesting that 3,3′-Diindolylmethane (DIM), a compound derived from the digestion of indole-3-carbinol, which is abundant in cruciferous vegetables, harbors anti-tumor activity in vitro and in vivo. Accumulating evidence suggests that AMP-activated protein kinase (AMPK) plays an essential role in cellular energy homeostasis and tumor development and that targeting AMPK may be a promising therapeutic option for cancer treatment in the clinic. We previously reported that a formulated DIM (BR-DIM; hereafter referred as B-DIM) with higher bioavailability was able to induce apoptosis and inhibit cell growth, angiogenesis, and invasion of prostate cancer cells. However, the precise molecular mechanism(s) for the anti-cancer effects of B-DIM have not been fully elucidated. In the present study, we investigated whether AMP-activated protein kinase (AMPK) is a molecular target of B-DIM in human prostate cancer cells. Our results showed, for the first time, that B-DIM could activate the AMPK signaling pathway, associated with suppression of the mammalian target of rapamycin (mTOR), down-regulation of androgen receptor (AR) expression, and induction of apoptosis in both androgen-sensitive LNCaP and androgen-insensitive C4-2B prostate cancer cells. B-DIM also activates AMPK and down-regulates AR in androgen-independent C4-2B prostate tumor xenografts in SCID mice. These results suggest that B-DIM could be used as a potential anti-cancer agent in the clinic for prevention and/or treatment of prostate cancer regardless of androgen responsiveness, although functional AR may be required. PMID:23056607

Lack of short-wavelength light during the school day delays dim light melatonin onset (DLMO) in middle school students.

PubMed

Figueiro, Mariana G; Rea, Mark S

2010-01-01

Circadian timing affects sleep onset. Delayed sleep onset can reduce sleep duration in adolescents required to awake early for a fixed school schedule. The absence of short-wavelength ("blue") morning light, which helps entrain the circadian system, can hypothetically delay sleep onset and decrease sleep duration in adolescents. The goal of this study was to investigate whether removal of short-wavelength light during the morning hours delayed the onset of melatonin in young adults. Dim light melatonin onset (DLMO) was measured in eleven 8th-grade students before and after wearing orange glasses, which removed short-wavelength light, for a five-day school week. DLMO was significantly delayed (30 minutes) after the five-day intervention, demonstrating that short-wavelength light exposure during the day can be important for advancing circadian rhythms in students. Lack of short-wavelength light in the morning has been shown to delay the circadian clock in controlled laboratory conditions. The results presented here are the first to show, outside laboratory conditions, that removal of short-wavelength light in the morning hours can delay DLMO in 8th-grade students. These field data, consistent with results from controlled laboratory studies, are directly relevant to lighting practice in schools.

Preliminary evidence that light through the eyelids can suppress melatonin and phase shift dim light melatonin onset.

PubMed

Figueiro, Mariana G; Rea, Mark S

2012-05-07

A previous study reported a method for measuring the spectral transmittance of individual human eyelids. A prototype light mask using narrow-band "green" light (λmax = 527 nm) was used to deliver light through closed eyelids in two within-subjects studies. The first study investigated whether an individual-specific light dose could suppress melatonin by 40% through the closed eyelid without disrupting sleep. The light doses were delivered at three times during the night: 1) beginning (while subjects were awake), 2) middle (during rapid eye movement (REM) sleep), and 3) end (during non-REM sleep). The second study investigated whether two individual-specific light doses expected to suppress melatonin by 30% and 60% and delivered through subjects' closed eyelids before the time of their predicted minimum core body temperature would phase delay the timing of their dim light melatonin onset (DLMO). Compared to a dark control night, light delivered through eyelids suppressed melatonin by 36% (p = 0.01) after 60-minute light exposure at the beginning, 45% (p = 0.01) at the middle, and 56% (p < 0.0001) at the end of the night. In the second study, compared to a dark control night, melatonin was suppressed by 25% (p = 0.03) and by 45% (p = 0.009) and circadian phase, as measured by DLMO, was delayed by 17 minutes (p = 0.03) and 71 minutes (ns) after 60-minute exposures to light levels 1 and 2, respectively. These studies demonstrate that individual-specific doses of light delivered through closed eyelids can suppress melatonin and phase shift DLMO and may be used to treat circadian sleep disorders.

Preliminary evidence that light through the eyelids can suppress melatonin and phase shift dim light melatonin onset

PubMed Central

2012-01-01

Background A previous study reported a method for measuring the spectral transmittance of individual human eyelids. A prototype light mask using narrow-band “green” light (λmax = 527 nm) was used to deliver light through closed eyelids in two within-subjects studies. The first study investigated whether an individual-specific light dose could suppress melatonin by 40% through the closed eyelid without disrupting sleep. The light doses were delivered at three times during the night: 1) beginning (while subjects were awake), 2) middle (during rapid eye movement (REM) sleep), and 3) end (during non-REM sleep). The second study investigated whether two individual-specific light doses expected to suppress melatonin by 30% and 60% and delivered through subjects’ closed eyelids before the time of their predicted minimum core body temperature would phase delay the timing of their dim light melatonin onset (DLMO). Findings Compared to a dark control night, light delivered through eyelids suppressed melatonin by 36% (p = 0.01) after 60-minute light exposure at the beginning, 45% (p = 0.01) at the middle, and 56% (p < 0.0001) at the end of the night. In the second study, compared to a dark control night, melatonin was suppressed by 25% (p = 0.03) and by 45% (p = 0.009) and circadian phase, as measured by DLMO, was delayed by 17 minutes (p = 0.03) and 71 minutes (ns) after 60-minute exposures to light levels 1 and 2, respectively. Conclusions These studies demonstrate that individual-specific doses of light delivered through closed eyelids can suppress melatonin and phase shift DLMO and may be used to treat circadian sleep disorders. PMID:22564396

Understanding CMEs using plasma diagnostics of the related dimmings

NASA Astrophysics Data System (ADS)

Vanninathan, Kamalam; Veronig, Astrid; Gomory, Peter; Dissauer, Karin; Temmer, Manuela; Hannah, Iain; Kontar, Eduard

2017-04-01

has weak upflows of 10 km/s. We conclude that the core dimming region corresponds to footpoints of the erupting flux rope from where there is continuous strong upflowing plasma for at least 10 hrs after the flare, while the secondary dimming region begins to refill within 1-2 hrs. These measurements can be used to deduce information about the mass of on-disk CMEs where white light measurements can fail. We also confirm that the dimmings are mainly caused by density decrease and not temperature changes. DEM analysis is a strong tool to decipher CME properties from dimming regions.

Calculating the dim light melatonin onset: the impact of threshold and sampling rate.

PubMed

Molina, Thomas A; Burgess, Helen J

2011-10-01

The dim light melatonin onset (DLMO) is the most reliable circadian phase marker in humans, but the cost of assaying samples is relatively high. Therefore, the authors examined differences between DLMOs calculated from hourly versus half-hourly sampling and differences between DLMOs calculated with two recommended thresholds (a fixed threshold of 3 pg/mL and a variable "3k" threshold equal to the mean plus two standard deviations of the first three low daytime points). The authors calculated these DLMOs from salivary dim light melatonin profiles collected from 122 individuals (64 women) at baseline. DLMOs derived from hourly sampling occurred on average only 6-8 min earlier than the DLMOs derived from half-hourly saliva sampling, and they were highly correlated with each other (r ≥ 0.89, p < .001). However, in up to 19% of cases the DLMO derived from hourly sampling was >30 min from the DLMO derived from half-hourly sampling. The 3 pg/mL threshold produced significantly less variable DLMOs than the 3k threshold. However, the 3k threshold was significantly lower than the 3 pg/mL threshold (p < .001). The DLMOs calculated with the 3k method were significantly earlier (by 22-24 min) than the DLMOs calculated with the 3 pg/mL threshold, regardless of sampling rate. These results suggest that in large research studies and clinical settings, the more affordable and practical option of hourly sampling is adequate for a reasonable estimate of circadian phase. Although the 3 pg/mL fixed threshold is less variable than the 3k threshold, it produces estimates of the DLMO that are further from the initial rise of melatonin.

Light at night alters daily patterns of cortisol and clock proteins in female Siberian hamsters.

PubMed

Bedrosian, T A; Galan, A; Vaughn, C A; Weil, Z M; Nelson, R J

2013-06-01

Humans and other organisms have adapted to a 24-h solar cycle in response to life on Earth. The rotation of the planet on its axis and its revolution around the sun cause predictable daily and seasonal patterns in day length. To successfully anticipate and adapt to these patterns in the environment, a variety of biological processes oscillate with a daily rhythm of approximately 24 h in length. These rhythms arise from hierarchally-coupled cellular clocks generated by positive and negative transcription factors of core circadian clock gene expression. From these endogenous cellular clocks, overt rhythms in activity and patterns in hormone secretion and other homeostatic processes emerge. These circadian rhythms in physiology and behaviour can be organised by a variety of cues, although they are most potently entrained by light. In recent history, there has been a major change from naturally-occurring light cycles set by the sun, to artificial and sometimes erratic light cycles determined by the use of electric lighting. Virtually every individual living in an industrialised country experiences light at night (LAN) but, despite its prevalence, the biological effects of such unnatural lighting have not been fully considered. Using female Siberian hamsters (Phodopus sungorus), we investigated the effects of chronic nightly exposure to dim light on daily rhythms in locomotor activity, serum cortisol concentrations and brain expression of circadian clock proteins (i.e. PER1, PER2, BMAL1). Although locomotor activity remained entrained to the light cycle, the diurnal fluctuation of cortisol concentrations was blunted and the expression patterns of clock proteins in the suprachiasmatic nucleus and hippocampus were altered. These results demonstrate that chronic exposure to dim LAN can dramatically affect fundamental cellular function and emergent physiology. © 2013 British Society for Neuroendocrinology.

Home dim light melatonin onsets with measures of compliance in delayed sleep phase disorder.

PubMed

Burgess, Helen J; Park, Margaret; Wyatt, James K; Fogg, Louis F

2016-06-01

The dim light melatonin onset (DLMO) assists with the diagnosis and treatment of circadian rhythm sleep disorders. Home DLMOs are attractive for cost savings and convenience, but can be confounded by home lighting and sample timing errors. We developed a home saliva collection kit with objective measures of light exposure and sample timing. We report on our first test of the kit in a clinical population. Thirty-two participants with delayed sleep phase disorder (DSPD; 17 women, aged 18-52 years) participated in two back-to-back home and laboratory phase assessments. Most participants (66%) received at least one 30-s epoch of light >50 lux during the home phase assessments, but for only 1.5% of the time. Most participants (56%) collected every saliva sample within 5 min of the scheduled time. Eighty-three per cent of home DLMOs were not affected by light or sampling errors. The home DLMOs occurred, on average, 10.2 min before the laboratory DLMOs, and were correlated highly with the laboratory DLMOs (r = 0.93, P < 0.001). These results indicate that home saliva sampling with objective measures of light exposure and sample timing, can assist in identifying accurate home DLMOs. © 2016 European Sleep Research Society.

The role of 11-cis-retinyl esters in vertebrate cone vision.

PubMed

Babino, Darwin; Perkins, Brian D; Kindermann, Aljoscha; Oberhauser, Vitus; von Lintig, Johannes

2015-01-01

A cycle of cis-to-trans isomerization of the chromophore is intrinsic to vertebrate vision where rod and cone photoreceptors mediate dim- and bright-light vision, respectively. Daylight illumination can greatly exceed the rate at which the photoproduct can be recycled back to the chromophore by the canonical visual cycle. Thus, an additional supply pathway(s) must exist to sustain cone-dependent vision. Two-photon microscopy revealed that the eyes of the zebrafish (Danio rerio) contain high levels of 11-cis-retinyl esters (11-REs) within the retinal pigment epithelium. HPLC analyses demonstrate that 11-REs are bleached by bright light and regenerated in the dark. Pharmacologic treatment with all-trans-retinylamine (Ret-NH2), a potent and specific inhibitor of the trans-to-cis reisomerization reaction of the canonical visual cycle, impeded the regeneration of 11-REs. Intervention with 11-cis-retinol restored the regeneration of 11-REs in the presence of all-trans-Ret-NH2. We used the XOPS:mCFP transgenic zebrafish line with a functional cone-only retina to directly demonstrate that this 11-RE cycle is critical to maintain vision under bright-light conditions. Thus, our analyses reveal that a dark-generated pool of 11-REs helps to supply photoreceptors with the chromophore under the varying light conditions present in natural environments. © FASEB.

Dim light at night disturbs the daily sleep-wake cycle in the rat.

PubMed

Stenvers, Dirk Jan; van Dorp, Rick; Foppen, Ewout; Mendoza, Jorge; Opperhuizen, Anne-Loes; Fliers, Eric; Bisschop, Peter H; Meijer, Johanna H; Kalsbeek, Andries; Deboer, Tom

2016-10-20

Exposure to light at night (LAN) is associated with insomnia in humans. Light provides the main input to the master clock in the hypothalamic suprachiasmatic nucleus (SCN) that coordinates the sleep-wake cycle. We aimed to develop a rodent model for the effects of LAN on sleep. Therefore, we exposed male Wistar rats to either a 12 h light (150-200lux):12 h dark (LD) schedule or a 12 h light (150-200 lux):12 h dim white light (5 lux) (LDim) schedule. LDim acutely decreased the amplitude of daily rhythms of REM and NREM sleep, with a further decrease over the following days. LDim diminished the rhythms of 1) the circadian 16-19 Hz frequency domain within the NREM sleep EEG, and 2) SCN clock gene expression. LDim also induced internal desynchronization in locomotor activity by introducing a free running rhythm with a period of ~25 h next to the entrained 24 h rhythm. LDim did not affect body weight or glucose tolerance. In conclusion, we introduce the first rodent model for disturbed circadian control of sleep due to LAN. We show that internal desynchronization is possible in a 24 h L:D cycle which suggests that a similar desynchronization may explain the association between LAN and human insomnia.

Dim light at night disturbs the daily sleep-wake cycle in the rat

PubMed Central

Jan Stenvers, Dirk; van Dorp, Rick; Foppen, Ewout; Mendoza, Jorge; Opperhuizen, Anne-Loes; Fliers, Eric; Bisschop, Peter H.; Meijer, Johanna H.; Kalsbeek, Andries; Deboer, Tom

2016-01-01

Exposure to light at night (LAN) is associated with insomnia in humans. Light provides the main input to the master clock in the hypothalamic suprachiasmatic nucleus (SCN) that coordinates the sleep-wake cycle. We aimed to develop a rodent model for the effects of LAN on sleep. Therefore, we exposed male Wistar rats to either a 12 h light (150–200lux):12 h dark (LD) schedule or a 12 h light (150–200 lux):12 h dim white light (5 lux) (LDim) schedule. LDim acutely decreased the amplitude of daily rhythms of REM and NREM sleep, with a further decrease over the following days. LDim diminished the rhythms of 1) the circadian 16–19 Hz frequency domain within the NREM sleep EEG, and 2) SCN clock gene expression. LDim also induced internal desynchronization in locomotor activity by introducing a free running rhythm with a period of ~25 h next to the entrained 24 h rhythm. LDim did not affect body weight or glucose tolerance. In conclusion, we introduce the first rodent model for disturbed circadian control of sleep due to LAN. We show that internal desynchronization is possible in a 24 h L:D cycle which suggests that a similar desynchronization may explain the association between LAN and human insomnia. PMID:27762290

Limited evidence for affective and diurnal rhythm responses to dim light-at-night in male and female C57Bl/6 mice.

PubMed

Cleary-Gaffney, Michael; Coogan, Andrew N

2018-05-15

Circadian rhythms are recurring patterns in a range of behavioural, physiological and molecular parameters that display periods of near 24 h, and are underpinned by an endogenous biological timekeeping system. Circadian clocks are increasingly recognised as being key for health. Environmental light is the key stimulus that synchronises the internal circadian system with the external time cues. There are emergent health concerns regarding increasing worldwide prevalence of electric lighting, especially man-made light-at-night, and light's impact on the circadian system may be central to these effects. A number of previous studies have demonstrated increased depression-like behaviour in various rodent experimental models exposed to dim light-at-night. In this study we set out to study the impact of dim light-at-night on circadian and affective behaviours in C57Bl/6 mice. We set out specifically to examine the impact of sex on light at night's effects, as well as the impact of housing conditions. We report minimal impact of light-at-night on circadian and affective behaviours, as measured by the tail suspension test, the forced swim test, the sucrose preference test and the elevated plus maze. Light-at-night was also not associated with an increase in body weight, but was associated with a decrease in the cell proliferation marker Ki-67 in the dentate gyrus. In summary, we conclude that experimental contextual factors, such as model species or strain, may be considerable importance in the investigation of the impact of light at night on mood-related parameters. Copyright © 2018 Elsevier Inc. All rights reserved.

The hockey-stick method to estimate evening dim light melatonin onset (DLMO) in humans.

PubMed

Danilenko, Konstantin V; Verevkin, Evgeniy G; Antyufeev, Viktor S; Wirz-Justice, Anna; Cajochen, Christian

2014-04-01

The onset of melatonin secretion in the evening is the most reliable and most widely used index of circadian timing in humans. Saliva (or plasma) is usually sampled every 0.5-1 hours under dim-light conditions in the evening 5-6 hours before usual bedtime to assess the dim-light melatonin onset (DLMO). For many years, attempts have been made to find a reliable objective determination of melatonin onset time either by fixed or dynamic threshold approaches. The here-developed hockey-stick algorithm, used as an interactive computer-based approach, fits the evening melatonin profile by a piecewise linear-parabolic function represented as a straight line switching to the branch of a parabola. The switch point is considered to reliably estimate melatonin rise time. We applied the hockey-stick method to 109 half-hourly melatonin profiles to assess the DLMOs and compared these estimates to visual ratings from three experts in the field. The DLMOs of 103 profiles were considered to be clearly quantifiable. The hockey-stick DLMO estimates were on average 4 minutes earlier than the experts' estimates, with a range of -27 to +13 minutes; in 47% of the cases the difference fell within ±5 minutes, in 98% within -20 to +13 minutes. The raters' and hockey-stick estimates showed poor accordance with DLMOs defined by threshold methods. Thus, the hockey-stick algorithm is a reliable objective method to estimate melatonin rise time, which does not depend on a threshold value and is free from errors arising from differences in subjective circadian phase estimates. The method is available as a computerized program that can be easily used in research settings and clinical practice either for salivary or plasma melatonin values.

Retinal and optical adaptations for nocturnal vision in the halictid bee Megalopta genalis.

PubMed

Greiner, Birgit; Ribi, Willi A; Warrant, Eric J

2004-06-01

The apposition compound eye of a nocturnal bee, the halictid Megalopta genalis, is described for the first time. Compared to the compound eye of the worker honeybee Apis mellifera and the diurnal halictid bee Lasioglossum leucozonium, the eye of M. genalis shows specific retinal and optical adaptations for vision in dim light. The major anatomical adaptations within the eye of the nocturnal bee are (1) nearly twofold larger ommatidial facets and (2) a 4-5 times wider rhabdom diameter than found in the diurnal bees studied. Optically, the apposition eye of M. genalis is 27 times more sensitive to light than the eyes of the diurnal bees. This increased optical sensitivity represents a clear optical adaptation to low light intensities. Although this unique nocturnal apposition eye has a greatly improved ability to catch light, a 27-fold increase in sensitivity alone cannot account for nocturnal vision at light intensities that are 8 log units dimmer than during daytime. New evidence suggests that additional neuronal spatial summation within the first optic ganglion, the lamina, is involved.

The intensity threshold of colour vision in a passerine bird, the blue tit (Cyanistes caeruleus).

PubMed

Gomez, Doris; Grégoire, Arnaud; Del Rey Granado, Maria; Bassoul, Marine; Degueldre, David; Perret, Philippe; Doutrelant, Claire

2014-11-01

Many vertebrates use colour vision for vital behaviour but their visual performance in dim light is largely unknown. The light intensity threshold of colour vision is known only for humans, horses and two parrot species. Here, we first explore this threshold in a passerine bird, the blue tit (Cyanistes caeruleus). Using classic conditioning of colour cues to food rewards in three individuals, we find a threshold ranging from 0.05 to 0.2 cd m(-2). Results are comparable to the two previously tested bird species. For tits, nest light conditions probably exceed that threshold, at least after sunrise. These results shed new light on the lively debate questioning the visual performance of cavity nesters and the evolutionary significance of egg and chick coloration. Although this needs further investigation, it is possible that blue tits exploit both colour and brightness cues when viewing their eggs, chicks or conspecifics in their nests. © 2014. Published by The Company of Biologists Ltd.

Neuropeptide Mapping of Dimmed Cells of Adult Drosophila Brain

NASA Astrophysics Data System (ADS)

Diesner, Max; Predel, Reinhard; Neupert, Susanne

2018-05-01

Neuropeptides are structurally highly diverse messenger molecules that act as regulators of many physiological processes such as development, metabolism, reproduction or behavior in general. Differentiation of neuropeptidergic cells often corresponds with the presence of the transcription factor DIMMED. In the central nervous system of the fruit fly Drosophila melanogaster, DIMMED commonly occurs in neuroendocrine neurons that release peptides as neurohormones but also in interneurons with complex branching patterns. Fly strains with green fluorescence protein (GFP)-expressing dimmed cells make it possible to systematically analyze the processed neuropeptides in these cells. In this study, we mapped individual GFP-expressing neurons of adult D. melanogaster from the dimmed ( c929)>GFP line. Using single cell mass spectrometry, we analyzed 10 types of dimmed neurons from the brain/gnathal ganglion. These cells included neuroendocrine cells with projection into the retrocerebral complex but also a number of large interneurons. Resulting mass spectra not only provided comprehensive data regarding mature products from 13 neuropeptide precursors but also evidence for the cellular co-localization of neuropeptides from different neuropeptide genes. The results can be implemented in a neuroanatomical map of the D. melanogaster brain. [Figure not available: see fulltext.

Neuropeptide Mapping of Dimmed Cells of Adult Drosophila Brain

NASA Astrophysics Data System (ADS)

Diesner, Max; Predel, Reinhard; Neupert, Susanne

2018-01-01

Neuropeptides are structurally highly diverse messenger molecules that act as regulators of many physiological processes such as development, metabolism, reproduction or behavior in general. Differentiation of neuropeptidergic cells often corresponds with the presence of the transcription factor DIMMED. In the central nervous system of the fruit fly Drosophila melanogaster, DIMMED commonly occurs in neuroendocrine neurons that release peptides as neurohormones but also in interneurons with complex branching patterns. Fly strains with green fluorescence protein (GFP)-expressing dimmed cells make it possible to systematically analyze the processed neuropeptides in these cells. In this study, we mapped individual GFP-expressing neurons of adult D. melanogaster from the dimmed (c929)>GFP line. Using single cell mass spectrometry, we analyzed 10 types of dimmed neurons from the brain/gnathal ganglion. These cells included neuroendocrine cells with projection into the retrocerebral complex but also a number of large interneurons. Resulting mass spectra not only provided comprehensive data regarding mature products from 13 neuropeptide precursors but also evidence for the cellular co-localization of neuropeptides from different neuropeptide genes. The results can be implemented in a neuroanatomical map of the D. melanogaster brain. [Figure not available: see fulltext.

Neuropeptide Mapping of Dimmed Cells of Adult Drosophila Brain.

PubMed

Diesner, Max; Predel, Reinhard; Neupert, Susanne

2018-05-01

Neuropeptides are structurally highly diverse messenger molecules that act as regulators of many physiological processes such as development, metabolism, reproduction or behavior in general. Differentiation of neuropeptidergic cells often corresponds with the presence of the transcription factor DIMMED. In the central nervous system of the fruit fly Drosophila melanogaster, DIMMED commonly occurs in neuroendocrine neurons that release peptides as neurohormones but also in interneurons with complex branching patterns. Fly strains with green fluorescence protein (GFP)-expressing dimmed cells make it possible to systematically analyze the processed neuropeptides in these cells. In this study, we mapped individual GFP-expressing neurons of adult D. melanogaster from the dimmed (c929)>GFP line. Using single cell mass spectrometry, we analyzed 10 types of dimmed neurons from the brain/gnathal ganglion. These cells included neuroendocrine cells with projection into the retrocerebral complex but also a number of large interneurons. Resulting mass spectra not only provided comprehensive data regarding mature products from 13 neuropeptide precursors but also evidence for the cellular co-localization of neuropeptides from different neuropeptide genes. The results can be implemented in a neuroanatomical map of the D. melanogaster brain. Graphical Abstract ᅟ.

Vision in the deep sea.

PubMed

Warrant, Eric J; Locket, N Adam

2004-08-01

The deep sea is the largest habitat on earth. Its three great faunal environments--the twilight mesopelagic zone, the dark bathypelagic zone and the vast flat expanses of the benthic habitat--are home to a rich fauna of vertebrates and invertebrates. In the mesopelagic zone (150-1000 m), the down-welling daylight creates an extended scene that becomes increasingly dimmer and bluer with depth. The available daylight also originates increasingly from vertically above, and bioluminescent point-source flashes, well contrasted against the dim background daylight, become increasingly visible. In the bathypelagic zone below 1000 m no daylight remains, and the scene becomes entirely dominated by point-like bioluminescence. This changing nature of visual scenes with depth--from extended source to point source--has had a profound effect on the designs of deep-sea eyes, both optically and neurally, a fact that until recently was not fully appreciated. Recent measurements of the sensitivity and spatial resolution of deep-sea eyes--particularly from the camera eyes of fishes and cephalopods and the compound eyes of crustaceans--reveal that ocular designs are well matched to the nature of the visual scene at any given depth. This match between eye design and visual scene is the subject of this review. The greatest variation in eye design is found in the mesopelagic zone, where dim down-welling daylight and bio-luminescent point sources may be visible simultaneously. Some mesopelagic eyes rely on spatial and temporal summation to increase sensitivity to a dim extended scene, while others sacrifice this sensitivity to localise pinpoints of bright bioluminescence. Yet other eyes have retinal regions separately specialised for each type of light. In the bathypelagic zone, eyes generally get smaller and therefore less sensitive to point sources with increasing depth. In fishes, this insensitivity, combined with surprisingly high spatial resolution, is very well adapted to the

Nocturnality constrains morphological and functional diversity in the eyes of reef fishes

PubMed Central

2011-01-01

Background Ambient light levels are often considered to drive the evolution of eye form and function. Diel activity pattern is the main mechanism controlling the visual environment of teleost reef fish, with day-active (diurnal) fish active in well-illuminated conditions, whereas night-active (nocturnal) fish cope with dim light. Physiological optics predicts several specific evolutionary responses to dim-light vision that should be reflected in visual performance features of the eye. Results We analyzed a large comparative dataset on morphological traits of the eyes in 265 species of teleost reef fish in 43 different families. The eye morphology of nocturnal reef teleosts is characterized by a syndrome that indicates better light sensitivity, including large relative eye size, high optical ratio and large, rounded pupils. Improved dim-light image formation comes at the cost of reduced depth of focus and reduction of potential accommodative lens movement. Diurnal teleost reef fish, released from the stringent functional requirements of dim-light vision have much higher morphological and optical diversity than nocturnal species, with large ranges of optical ratio, depth of focus, and lens accommodation. Conclusions Physical characteristics of the environment are an important factor in the evolution and diversification of the vertebrate eye. Both teleost reef fish and terrestrial amniotes meet the functional requirements of dim-light vision with a similar evolutionary response of morphological and optical modifications. The trade-off between improved dim-light vision and reduced optical diversity may be a key factor in explaining the lower trophic diversity of nocturnal reef teleosts. PMID:22098687

Nocturnality constrains morphological and functional diversity in the eyes of reef fishes.

PubMed

Schmitz, Lars; Wainwright, Peter C

2011-11-19

Ambient light levels are often considered to drive the evolution of eye form and function. Diel activity pattern is the main mechanism controlling the visual environment of teleost reef fish, with day-active (diurnal) fish active in well-illuminated conditions, whereas night-active (nocturnal) fish cope with dim light. Physiological optics predicts several specific evolutionary responses to dim-light vision that should be reflected in visual performance features of the eye. We analyzed a large comparative dataset on morphological traits of the eyes in 265 species of teleost reef fish in 43 different families. The eye morphology of nocturnal reef teleosts is characterized by a syndrome that indicates better light sensitivity, including large relative eye size, high optical ratio and large, rounded pupils. Improved dim-light image formation comes at the cost of reduced depth of focus and reduction of potential accommodative lens movement. Diurnal teleost reef fish, released from the stringent functional requirements of dim-light vision have much higher morphological and optical diversity than nocturnal species, with large ranges of optical ratio, depth of focus, and lens accommodation. Physical characteristics of the environment are an important factor in the evolution and diversification of the vertebrate eye. Both teleost reef fish and terrestrial amniotes meet the functional requirements of dim-light vision with a similar evolutionary response of morphological and optical modifications. The trade-off between improved dim-light vision and reduced optical diversity may be a key factor in explaining the lower trophic diversity of nocturnal reef teleosts.

Dark nights reverse metabolic disruption caused by dim light at night.

PubMed

Fonken, L K; Weil, Z M; Nelson, R J

2013-06-01

The increasing prevalence of obesity and related metabolic disorders coincides with increasing exposure to light at night. Previous studies report that mice exposed to dim light at night (dLAN) develop symptoms of metabolic syndrome. This study investigated whether mice returned to dark nights after dLAN exposure recover metabolic function. Male Swiss-Webster mice were assigned to either: standard light-dark (LD) conditions for 8 weeks (LD/LD), dLAN for 8 weeks (dLAN/dLAN), LD for 4 weeks followed by 4 weeks of dLAN (LD/dLAN), and dLAN for 4 weeks followed by 4 weeks of LD (dLAN/LD). After 4 weeks in their respective lighting conditions both groups initially placed in dLAN increased body mass gain compared to LD mice. Half of the dLAN mice (dLAN/LD) were then transferred to LD and vice versa (LD/dLAN). Following the transfer dLAN/dLAN and LD/dLAN mice gained more weight than LD/LD and dLAN/LD mice. At the conclusion of the study dLAN/LD mice did not differ from LD/LD mice with respect to weight gain and had lower fat pad mass compared to dLAN/dLAN mice. Compared to all other groups dLAN/dLAN mice decreased glucose tolerance as indicated by an intraperitoneal glucose tolerance test at week 7, indicating that dLAN/LD mice recovered glucose metabolism. dLAN/dLAN mice also increased MAC1 mRNA expression in peripheral fat as compared to both LD/LD and dLAN/LD mice, suggesting peripheral inflammation is induced by dLAN, but not sustained after return to LD. These results suggest that re-exposure to dark nights ameliorates metabolic disruption caused by dLAN exposure. Copyright © 2013 The Obesity Society.

Relationship of EUV Irradiance Coronal Dimming Slope and Depth to Coronal Mass Ejection Speed and Mass

NASA Technical Reports Server (NTRS)

Mason, James Paul; Woods, Thomas N.; Webb, David F.; Thompson, Barbara J.; Colaninno, Robin C.; Vourlidas, Angelos

2016-01-01

Extreme ultraviolet (EUV) coronal dimmings are often observed in response to solar eruptive events. These phenomena can be generated via several different physical processes. For space weather, the most important of these is the temporary void left behind by a coronal mass ejection (CME). Massive, fast CMEs tend to leave behind a darker void that also usually corresponds to minimum irradiance for the cooler coronal emissions. If the dimming is associated with a solar are, as is often the case, the are component of the irradiance light curve in the cooler coronal emission can be isolated and removed using simultaneous measurements of warmer coronal lines. We apply this technique to 37dimming events identified during two separate two-week periods in 2011, plus an event on 2010 August 7 analyzed in a previous paper, to parameterize dimming in terms of depth and slope. We provide statistics on which combination of wavelengths worked best for the flare-removal method, describe the fitting methods applied to the dimming light curves, and compare the dimming parameters with corresponding CME parameters of mass and speed. The best linear relationships found are nu(sub CME) [km/s] approx. equals 2.36 x 10 6 [km/%] x s(sub dim) [%/s] m(sub CME) [g] approx. equals 2.59 x 10(exp.15 [g/%] x the square root of d(sub dim) [%].These relationships could be used for space weather operations of estimating CME mass and speed using near-real-time irradiance dimming measurements.

Retail Lamps Study 3.1: Dimming, Flicker, and Power Quality Characteristics of LED A Lamps

DOE Office of Scientific and Technical Information (OSTI.GOV)

Royer, Michael P.; Poplawski, Michael E.; Brown, Charles C.

2014-12-01

To date, all three reports in the retail lamps series have focused on basic performance parameters, such as lumen output, efficacy, and color quality. This report goes a step further, examining the photoelectric characteristics (i.e., dimming and flicker) of a subset of lamps from CALiPER Retails Lamps Study 3. Specifically, this report focuses on the dimming, power quality, and flicker characteristics of 14 LED A lamps, as controlled by four different retail-available dimmers. The results demonstrate notable variation across the various lamps, but little variation between the four dimmers. Overall, the LED lamps: ~tended to have higher relative light outputmore » compared to the incandescent and halogen benchmark at the same dimmer output signal (RMS voltage). The lamps’ dimming curves (i.e., the relationship between control signal and relative light output) ranged from linear to very similar to the square-law curve typical of an incandescent lamp. ~generally exhibited symmetrical behavior—the same dimming curve—when measured proceeding from maximum to minimum or minimum to maximum control signal. ~mostly dimmed below 10% of full light output, with some exceptions for specific lamp and dimmer combinations ~exhibited a range of flicker characteristics, with many comparing favorably to the level typical of a magnetically-ballasted fluorescent lamp through at least a majority of the dimming range. ~ always exceeded the relative (normalized) efficacy over the dimming range of the benchmark lamps, which rapidly decline in efficacy when they are dimmed. This report generally does not attempt to rank the performance of one product compared to another, but instead focuses on the collective performance of the group versus conventional incandescent or halogen lamps, the performance of which is likely to be the baseline for a majority of consumers. Undoubtedly, some LED lamps perform better—or more similar to conventional lamps—than others. Some perform desirably

Mini-review: Far peripheral vision.

PubMed

Simpson, Michael J

2017-11-01

The region of far peripheral vision, beyond 60 degrees of visual angle, is important to the evaluation of peripheral dark shadows (negative dysphotopsia) seen by some intraocular lens (IOL) patients. Theoretical calculations show that the limited diameter of an IOL affects ray paths at large angles, leading to a dimming of the main image for small pupils, and to peripheral illumination by light bypassing the IOL for larger pupils. These effects are rarely bothersome, and cataract surgery is highly successful, but there is a need to improve the characterization of far peripheral vision, for both pseudophakic and phakic eyes. Perimetry is the main quantitative test, but the purpose is to evaluate pathologies rather than characterize vision (and object and image regions are no longer uniquely related in the pseudophakic eye). The maximum visual angle is approximately 105 0 , but there is limited information about variations with age, race, or refractive error (in case there is an unexpected link with the development of myopia), or about how clear cornea, iris location, and the limiting retina are related. Also, the detection of peripheral motion is widely recognized to be important, yet rarely evaluated. Overall, people rarely complain specifically about this visual region, but with "normal" vision including an IOL for >5% of people, and increasing interest in virtual reality and augmented reality, there are new reasons to characterize peripheral vision more completely. Copyright © 2017 Elsevier Ltd. All rights reserved.

Experiment S030: Dim sky photography/orthicon

NASA Technical Reports Server (NTRS)

Dunkelman, L.; Mercer, R. D.; Ney, E. P.; Hemenway, C. L.

1971-01-01

During Gemini missions, the image orthicon system was used to obtain photographic data on faint and diffuse astronomical phenomena. Results show that the photographs may be used to determine the airglow geometry. Although it was sensitive, the original photographic system was unsuitable for use in the study of dim and diffuse astronomical light sources.

Estimating dim light melatonin onset (DLMO) phase in adolescents using summer or school-year sleep/wake schedules.

PubMed

Crowley, Stephanie J; Acebo, Christine; Fallone, Gahan; Carskadon, Mary A

2006-12-01

This analysis examined associations between the salivary dim light melatonin onset (DLMO) phase and self-selected sleep/ wake schedules in groups of children and adolescents during summer vacation and during the school year to determine the degree to which sleep/wake patterns can estimate salivary DLMO phase. Participants slept at home on self-selected schedules for 5 consecutive nights and reported their bedtime and wake-up time via daily telephone messages. Salivary melatonin was sampled in the laboratory on one evening every 30 minutes in dim light (< 50 lux) to determine DLMO phase. Within group bivariate regressions between sleep pattern measures (bedtime, wake-up time, and midsleep time) and DLMO phase were computed. One group, ages 9 to 17 years (mean age = 12.5, SD = 2.3 years, 74 males, 75 females) contributed 149 DLMO phase and sleep/wake pattern measures while on a school year schedule ("school group"). A separate group, ages 9 to 16 years (mean age = 13.1, SD = 1.3 years, 30 males, 29 females) contributed 59 DLMO phase and sleep/wake pattern measures while on a summer schedule ("summer group"). Bedtime, midsleep time, and wake-up time were positively correlated with DLMO phase in both groups. Although all correlation coefficients for the summer group were statistically greater compared to the school group, the regression equations predicted DLMO phase within +/- 1 hour of the measured DLMO phase in approximately 80% for both groups. DLMO phase can be estimated using self-selected sleep/wake patterns during the school year or summer vacation in healthy children and adolescents.

Dim light at night disrupts the short-day response in Siberian hamsters.

PubMed

Ikeno, Tomoko; Weil, Zachary M; Nelson, Randy J

2014-02-01

Photoperiodic regulation of physiology, morphology, and behavior is crucial for many animals to survive seasonally variable conditions unfavorable for reproduction and survival. The photoperiodic response in mammals is mediated by nocturnal secretion of melatonin under the control of a circadian clock. However, artificial light at night caused by recent urbanization may disrupt the circadian clock, as well as the photoperiodic response by blunting melatonin secretion. Here we examined the effect of dim light at night (dLAN) (5lux of light during the dark phase) on locomotor activity rhythms and short-day regulation of reproduction, body mass, pelage properties, and immune responses of male Siberian hamsters. Short-day animals reduced gonadal and body mass, decreased spermatid nuclei and sperm numbers, molted to a whiter pelage, and increased pelage density compared to long-day animals. However, animals that experienced short days with dLAN did not show these short-day responses. Moreover, short-day specific immune responses were altered in dLAN conditions. The nocturnal activity pattern was blunted in dLAN hamsters, consistent with the observation that dLAN changed expression of the circadian clock gene, Period1. In addition, we demonstrated that expression levels of genes implicated in the photoperiodic response, Mel-1a melatonin receptor, Eyes absent 3, thyroid stimulating hormone receptor, gonadotropin-releasing hormone, and gonadotropin-inhibitory hormone, were higher in dLAN animals than those in short-day animals. These results suggest that dLAN disturbs the circadian clock function and affects the molecular mechanisms of the photoperiodic response. Copyright © 2013 Elsevier Inc. All rights reserved.

Restoration of vision after transplantation of photoreceptors.

PubMed

Pearson, R A; Barber, A C; Rizzi, M; Hippert, C; Xue, T; West, E L; Duran, Y; Smith, A J; Chuang, J Z; Azam, S A; Luhmann, U F O; Benucci, A; Sung, C H; Bainbridge, J W; Carandini, M; Yau, K-W; Sowden, J C; Ali, R R

2012-05-03

Cell transplantation is a potential strategy for treating blindness caused by the loss of photoreceptors. Although transplanted rod-precursor cells are able to migrate into the adult retina and differentiate to acquire the specialized morphological features of mature photoreceptor cells, the fundamental question remains whether transplantation of photoreceptor cells can actually improve vision. Here we provide evidence of functional rod-mediated vision after photoreceptor transplantation in adult Gnat1−/− mice, which lack rod function and are a model of congenital stationary night blindness. We show that transplanted rod precursors form classic triad synaptic connections with second-order bipolar and horizontal cells in the recipient retina. The newly integrated photoreceptor cells are light-responsive with dim-flash kinetics similar to adult wild-type photoreceptors. By using intrinsic imaging under scotopic conditions we demonstrate that visual signals generated by transplanted rods are projected to higher visual areas, including V1. Moreover, these cells are capable of driving optokinetic head tracking and visually guided behaviour in the Gnat1−/− mouse under scotopic conditions. Together, these results demonstrate the feasibility of photoreceptor transplantation as a therapeutic strategy for restoring vision after retinal degeneration.

Behavioural and physiological limits to vision in mammals

PubMed Central

Field, Greg D.

2017-01-01

Human vision is exquisitely sensitive—a dark-adapted observer is capable of reliably detecting the absorption of a few quanta of light. Such sensitivity requires that the sensory receptors of the retina, rod photoreceptors, generate a reliable signal when single photons are absorbed. In addition, the retina must be able to extract this information and relay it to higher visual centres under conditions where very few rods signal single-photon responses while the majority generate only noise. Critical to signal transmission are mechanistic optimizations within rods and their dedicated retinal circuits that enhance the discriminability of single-photon responses by mitigating photoreceptor and synaptic noise. We describe behavioural experiments over the past century that have led to the appreciation of high sensitivity near absolute visual threshold. We further consider mechanisms within rod photoreceptors and dedicated rod circuits that act to extract single-photon responses from cellular noise. We highlight how these studies have shaped our understanding of brain function and point out several unresolved questions in the processing of light near the visual threshold. This article is part of the themed issue ‘Vision in dim light’. PMID:28193817

Effects of Solar Dimming and Brightening on the Terrestrial Carbon Sink

NASA Astrophysics Data System (ADS)

Mercado, L. M.; Bellouin, N.; Sitch, S.; Boucher, O.; Huntingford, C.; Cox, P. M.

2008-12-01

A decrease in total solar radiation (Liepert, 2002, Stanhill and Cohen, 2001, Wild et al., 2005) has been observed at the earth surface over the 1950-1990 period, called solar dimming. Such dimming gradually started to transform into brightening in some regions of the world since the late 1980s (Wild et al. 2005). Both dimming and brightening are likely to be linked to an increase and decrease in cloud cover and scattering and absorption of light by tropospheric and stratospheric aerosols respectively (Kvalevag and Myhre, 2007). Theoretical and observational studies have shown that plant photosynthesis of forest and crop ecosystems is more efficient under diffuse light conditions (Gu et al., 2003, Niyogi et al., 2004, Oliveira et al., 2007, Roderick et al., 2001). However, this effect has not yet been accounted for in global carbon cycle simulations because such models lack the mechanism that includes the diffuse irradiance effects on photosynthesis. The aim of this study is to estimate the impact of changes in radiation during the 1900-2100 period on land productivity and carbon storage. We use an offline version of the land surface scheme of the Hadley centre model (Mercado et al., 2007) which has been modified to account for variations of direct and diffuse radiation on sunlit and shaded canopy photosynthesis. Additionally, we use short wave and photosynthetic active radiation fields reconstructed from the Hadley centre climate model which takes into account the scattering and absorption of light by tropospheric and stratospheric aerosols. We describe the simulation of the land carbon cycle through the dimming-brightening periods, and diagnose the impact that changes in diffuse radiation had on the land carbon sink. We also discuss the implications of these results for the future land carbon-sink, under likely changes in the atmospheric aerosol loading.

Light, Imaging, Vision: An interdisciplinary undergraduate course

NASA Astrophysics Data System (ADS)

Nelson, Philip

Students in physical and life science, and in engineering, need to know about the physics and biology of light. In the 21st century, it has become increasingly clear that the quantum nature of light is essential both for the latest imaging modalities and even to advance our knowledge of fundamental processes, such as photosynthesis and human vision. But many optics courses remain rooted in classical physics, with photons as an afterthought. I'll describe a new undergraduate course, for students in several science and engineering majors, that takes students from the rudiments of probability theory to modern methods like fluorescence imaging and Förster resonance energy transfer. After a digression into color vision, students then see how the Feynman principle explains the apparently wavelike phenomena associated to light, including applications like diffraction limit, subdiffraction imaging, total internal reflection and TIRF microscopy. Then we see how scientists documented the single-quantum sensitivity of the eye seven decades earlier than `ought' to have been possible, and finally close with the remarkable signaling cascade that delivers such outstanding performance. A new textbook embodying this course will be published by Princeton University Press in Spring 2017. Partially supported by the United States National Science Foundation under Grant PHY-1601894.

Dim light at night interacts with intermittent hypoxia to alter cognitive and affective responses.

PubMed

Aubrecht, Taryn G; Weil, Zachary M; Magalang, Ulysses J; Nelson, Randy J

2013-07-01

Obstructive sleep apnea (OSA) and dim light at night (dLAN) have both been independently associated with alterations in mood and cognition. We aimed to determine whether dLAN would interact with intermittent hypoxia (IH), a condition characteristic of OSA, to alter the behavioral, cognitive, and affective responses. Adult male mice were housed in either standard lighting conditions (14:10-h light-dark cycle; 150 lux:0 lux) or dLAN (150 lux:5 lux). Mice were then exposed to IH (15 cycles/h, 8 h/day, FiO2 nadir of 5%) for 3 wk, then tested in assays of affective and cognitive responses; brains were collected for dendritic morphology and PCR analysis. Exposure to dLAN and IH increased anxiety-like behaviors, as assessed in the open field, elevated plus maze, and the light/dark box. dLAN and IH increased depressive-like behaviors in the forced swim test. IH impaired learning and memory performance in the passive avoidance task; however, no differences were observed in spatial working memory, as assessed by y-maze or object recognition. IH combined with dLAN decreased cell body area in the CA1 and CA3 regions of the hippocampus. Overall, IH decreased apical spine density in the CA3, whereas dLAN decreased spine density in the CA1 of the hippocampus. TNF-α gene expression was not altered by IH or lighting condition, whereas VEGF expression was increased by dLAN. The combination of IH and dLAN provokes negative effects on hippocampal dendritic morphology, affect, and cognition, suggesting that limiting nighttime exposure to light in combination with other established treatments may be of benefit to patients with OSA.

Dim light at night interacts with intermittent hypoxia to alter cognitive and affective responses

PubMed Central

Weil, Zachary M.; Magalang, Ulysses J.; Nelson, Randy J.

2013-01-01

Obstructive sleep apnea (OSA) and dim light at night (dLAN) have both been independently associated with alterations in mood and cognition. We aimed to determine whether dLAN would interact with intermittent hypoxia (IH), a condition characteristic of OSA, to alter the behavioral, cognitive, and affective responses. Adult male mice were housed in either standard lighting conditions (14:10-h light-dark cycle; 150 lux:0 lux) or dLAN (150 lux:5 lux). Mice were then exposed to IH (15 cycles/h, 8 h/day, FiO2 nadir of 5%) for 3 wk, then tested in assays of affective and cognitive responses; brains were collected for dendritic morphology and PCR analysis. Exposure to dLAN and IH increased anxiety-like behaviors, as assessed in the open field, elevated plus maze, and the light/dark box. dLAN and IH increased depressive-like behaviors in the forced swim test. IH impaired learning and memory performance in the passive avoidance task; however, no differences were observed in spatial working memory, as assessed by y-maze or object recognition. IH combined with dLAN decreased cell body area in the CA1 and CA3 regions of the hippocampus. Overall, IH decreased apical spine density in the CA3, whereas dLAN decreased spine density in the CA1 of the hippocampus. TNF-α gene expression was not altered by IH or lighting condition, whereas VEGF expression was increased by dLAN. The combination of IH and dLAN provokes negative effects on hippocampal dendritic morphology, affect, and cognition, suggesting that limiting nighttime exposure to light in combination with other established treatments may be of benefit to patients with OSA. PMID:23657638

Infrared dim and small target detecting and tracking method inspired by Human Visual System

NASA Astrophysics Data System (ADS)

Dong, Xiabin; Huang, Xinsheng; Zheng, Yongbin; Shen, Lurong; Bai, Shengjian

2014-01-01

Detecting and tracking dim and small target in infrared images and videos is one of the most important techniques in many computer vision applications, such as video surveillance and infrared imaging precise guidance. Recently, more and more algorithms based on Human Visual System (HVS) have been proposed to detect and track the infrared dim and small target. In general, HVS concerns at least three mechanisms including contrast mechanism, visual attention and eye movement. However, most of the existing algorithms simulate only a single one of the HVS mechanisms, resulting in many drawbacks of these algorithms. A novel method which combines the three mechanisms of HVS is proposed in this paper. First, a group of Difference of Gaussians (DOG) filters which simulate the contrast mechanism are used to filter the input image. Second, a visual attention, which is simulated by a Gaussian window, is added at a point near the target in order to further enhance the dim small target. This point is named as the attention point. Eventually, the Proportional-Integral-Derivative (PID) algorithm is first introduced to predict the attention point of the next frame of an image which simulates the eye movement of human being. Experimental results of infrared images with different types of backgrounds demonstrate the high efficiency and accuracy of the proposed method to detect and track the dim and small targets.

A comparative study of rhodopsin function in the great bowerbird (Ptilonorhynchus nuchalis): Spectral tuning and light-activated kinetics.

PubMed

van Hazel, Ilke; Dungan, Sarah Z; Hauser, Frances E; Morrow, James M; Endler, John A; Chang, Belinda S W

2016-07-01

Rhodopsin is the visual pigment responsible for initiating the phototransduction cascade in vertebrate rod photoreceptors. Although well-characterized in a few model systems, comparative studies of rhodopsin function, particularly for nonmammalian vertebrates are comparatively lacking. Bowerbirds are rare among passerines in possessing a key substitution, D83N, at a site that is otherwise highly conserved among G protein-coupled receptors. While this substitution is present in some dim-light adapted vertebrates, often accompanying another unusual substitution, A292S, its functional relevance in birds is uncertain. To investigate functional effects associated with these two substitutions, we use the rhodopsin gene from the great bowerbird (Ptilonorhynchus nuchalis) as a background for site-directed mutagenesis, in vitro expression and functional characterization. We also mutated these sites in two additional rhodopsins that do not naturally possess N83, chicken and bovine, for comparison. Both sites were found to contribute to spectral blue-shifts, but had opposing effects on kinetic rates. Substitutions at site 83 were found to primarily affect the kinetics of light-activated rhodopsin, while substitutions at site 292 had a larger impact on spectral tuning. The contribution of substitutions at site 83 to spectral tuning in particular depended on genetic background, but overall, the effects of substitutions were otherwise surprisingly additive, and the magnitudes of functional shifts were roughly similar across all three genetic backgrounds. By employing a comparative approach with multiple species, our study provides new insight into the joint impact of sites 83 and 292 on rhodopsin structure-function as well as their evolutionary significance for dim-light vision across vertebrates. © 2016 The Protein Society.

Mystery of a Dimming White Dwarf

NASA Astrophysics Data System (ADS)

Kohler, Susanna

2015-12-01

In the wake of the recent media attention over an enigmatic, dimming star, another intriguing object has been discovered: J1529+2928, a white dwarf that periodically dims. This mystery, however, may have a simple solution with interesting consequences for future surveys of white dwarfs.Unexpected VariabilityJ1529+2928 is an isolated white dwarf that appears to have a mass of slightly more than the Sun. But rather than radiating steadily, J1529+2928 dims once every 38 minutes almost as though it were being eclipsed.The team that discovered these variations, led by Mukremin Kilic (University of Oklahoma), used telescopes at the Apache Point Observatory and the McDonald Observatory to obtain follow-up photometric data of J1529+2928 spread across 66 days. The team also took spectra of the white dwarf with the Gemini North telescope.Kilic and collaborators then began, one by one, to rule out possible causes of this objects variability.Eliminating OptionsThe period of the variability is too long for J1529+2928 to be a pulsating white dwarf with luminosity variation caused by gravity-wave pulsations.The variability cant be due to an eclipse by a stellar or brown-dwarf companion, because there isnt any variation in J1529+2928s radial velocity.Its not due to the orbit of a solid-body planetary object; such a transit would be too short to explain observations.It cant be due to the orbit of a disintegrated planet; this wouldnt explain the light curves observed in different filters plus the light curve doesnt change over the 66-day span.Spotty SurfaceTop and middle two panels: light curves from three different nights observing J1529+2928s periodic dimming. Bottom panel: The Fourier transform shows a peak at 37.7 cycles/day (and another, smaller peak at its first harmonic). [Kilic et al. 2015]So what explanation is left? The authors suggest that J1529+2928s variability is likely caused by a starspot on the white dwarfs surface that rotates into and out of our view. Estimates

Effect of light intensity on flight control and temporal properties of photoreceptors in bumblebees.

PubMed

Reber, Therese; Vähäkainu, Antti; Baird, Emily; Weckström, Matti; Warrant, Eric; Dacke, Marie

2015-05-01

To control flight, insects rely on the pattern of visual motion generated on the retina as they move through the environment. When light levels fall, vision becomes less reliable and flight control thus becomes more challenging. Here, we investigated the effect of light intensity on flight control by filming the trajectories of free-flying bumblebees (Bombus terrestris, Linnaeus 1758) in an experimental tunnel at different light levels. As light levels fell, flight speed decreased and the flight trajectories became more tortuous but the bees were still remarkably good at centring their flight about the tunnel's midline. To investigate whether this robust flight performance can be explained by visual adaptations in the bumblebee retina, we also examined the response speed of the green-sensitive photoreceptors at the same light intensities. We found that the response speed of the photoreceptors significantly decreased as light levels fell. This indicates that bumblebees have both behavioural (reduction in flight speed) and retinal (reduction in response speed of the photoreceptors) adaptations to allow them to fly in dim light. However, the more tortuous flight paths recorded in dim light suggest that these adaptations do not support flight with the same precision during the twilight hours of the day. © 2015. Published by The Company of Biologists Ltd.

Aural-Nondetectability Model Predictions for Night-Vision Goggles across Ambient Lighting Conditions

DTIC Science & Technology

2015-12-01

ARL-TR-7564 ● DEC 2015 US Army Research Laboratory Aural-Nondetectability Model Predictions for Night -Vision Goggles across...ARL-TR-7564 ● DEC 2015 US Army Research Laboratory Aural-Nondetectability Model Predictions for Night -Vision Goggles across Ambient...May 2015–30 Sep 2015 4. TITLE AND SUBTITLE Aural-Nondetectability Model Predictions for Night -Vision Goggles across Ambient Lighting Conditions 5a

Effect of Sitting Pause Times on Balance After Supine to Standing Transfer in Dim Light.

PubMed

Johnson, Eric G; Albalwi, Abdulaziz A; Al-Dabbak, Fuad M; Daher, Noha S

2017-06-01

The risk of falling for older adults increases in dimly lit environments. Longer sitting pause times, before getting out of bed and standing during the night, may improve postural stability. The purpose of this study was to measure the effect of sitting pause times on postural sway velocity immediately after a supine to standing transfer in a dimly lit room in older adult women. Eighteen healthy women aged 65 to 75 years who were able to independently perform supine to standing transfers participated in the study. On each of 2 consecutive days, participants assumed the supine position on a mat table and closed their eyes for 45 minutes. Then, participants were instructed to open their eyes and transfer from supine to sitting, with either 2- or 30-second pause in the sitting position followed by standing. The sitting pause time order was randomized. A significant difference was observed in postural sway velocity between the 2- and 30-second sitting pause times. The results revealed that there was less postural sway velocity after 30-second than 2-second sitting pause time (0.61 ± 0.19 vs 1.22 ± 0.68, P < .001). Falls related to bathroom usage at night are the most common reported falls among older adults. In the present study, the investigators studied the effect of sitting pause times on postural sway velocity after changing position from supine to standing in a dimly lit environment. The findings showed that the mean postural sway velocity was significantly less after 30-second sitting pause time compared with 2-second sitting pause time. Postural sway velocity decreased when participants performed a sitting pause of 30 seconds before standing in a dimly lit environment. These results suggest that longer sitting pause times may improve adaptability to dimly lit environments, contributing to improved postural stability and reduced risk of fall in older adult women when getting out of bed at night.

Parental Exposure to Dim Light at Night Prior to Mating Alters Offspring Adaptive Immunity.

PubMed

Cissé, Yasmine M; Russart, Kathryn L G; Nelson, Randy J

2017-03-31

Exposure to dim light at night (dLAN) disrupts natural light/dark cycles and impairs endogenous circadian rhythms necessary to maintain optimal biological function, including the endocrine and immune systems. We have previously demonstrated that white dLAN compromises innate and cell mediated immune responses in adult Siberian hamsters (Phodopus sungorus). We hypothesized that dLAN has transgenerational influences on immune function. Adult male and female Siberian hamsters were exposed to either dark nights (DARK) or dLAN (~5 lux) for 9 weeks, then paired in full factorial design, mated, and thereafter housed under dark nights. Offspring were gestated and reared in dark nights, then tested as adults for cell-mediated and humoral immunity. Maternal exposure to dLAN dampened delayed type hypersensitivity (DTH) responses in male offspring. Maternal and paternal exposure to dLAN reduced DTH responses in female offspring. IgG antibodies to a novel antigen were elevated in offspring of dams exposed to dLAN. Paternal exposure to dLAN decreased splenic endocrine receptor expression and global methylation in a parental sex-specific manner. Together, these data suggest that exposure to dLAN has transgenerational effects on endocrine-immune function that may be mediated by global alterations in the epigenetic landscape of immune tissues.

Parental Exposure to Dim Light at Night Prior to Mating Alters Offspring Adaptive Immunity

PubMed Central

Cissé, Yasmine M.; Russart, Kathryn L.G.; Nelson, Randy J.

2017-01-01

Exposure to dim light at night (dLAN) disrupts natural light/dark cycles and impairs endogenous circadian rhythms necessary to maintain optimal biological function, including the endocrine and immune systems. We have previously demonstrated that white dLAN compromises innate and cell mediated immune responses in adult Siberian hamsters (Phodopus sungorus). We hypothesized that dLAN has transgenerational influences on immune function. Adult male and female Siberian hamsters were exposed to either dark nights (DARK) or dLAN (~5 lux) for 9 weeks, then paired in full factorial design, mated, and thereafter housed under dark nights. Offspring were gestated and reared in dark nights, then tested as adults for cell-mediated and humoral immunity. Maternal exposure to dLAN dampened delayed type hypersensitivity (DTH) responses in male offspring. Maternal and paternal exposure to dLAN reduced DTH responses in female offspring. IgG antibodies to a novel antigen were elevated in offspring of dams exposed to dLAN. Paternal exposure to dLAN decreased splenic endocrine receptor expression and global methylation in a parental sex-specific manner. Together, these data suggest that exposure to dLAN has transgenerational effects on endocrine-immune function that may be mediated by global alterations in the epigenetic landscape of immune tissues. PMID:28361901

Characteristics of EIT Dimmings in Solar Eruptions

NASA Technical Reports Server (NTRS)

Adams, Mitzi; Sterling, A. C.

2006-01-01

Intensity "dimmings" in coronal images are a key feature of solar eruptions. Such dimmings are likely the source locations for much of the material expelled in coronal mass ejections (CMEs). Characteristics such as the timing of the dimmings with respect to the onset of other eruption signatures, and the location of the dimmings in the context of the magnetic field environment of the erupting region, are indicative of the mechanism leading to the eruption. We examine dimmings of six eruptions in images from the EUV Imaging Telescope (EIT) on SOHO, along with supplementary soft X-ray (SXR) data from GOES and the SXR Telescope (SXT) on Yohkoh. We examine the timing of the dimming onset and compare with the time of EUV and SXR brightening and determine the timescale for the recovery from dimming for each event. With line-of-sight photospheric magnetograms from the MDI instrument on SOHO, we determine the magnetic structure of the erupting regions and the locations of the dimmings in those regions. From our analysis we consider which mechanism likely triggered each eruption: internal tether cutting, external tether cutting ("breakout"), loss of equilibrium, or some other mechanism.

The Light Plane Calibration Method of the Laser Welding Vision Monitoring System

NASA Astrophysics Data System (ADS)

Wang, B. G.; Wu, M. H.; Jia, W. P.

2018-03-01

According to the aerospace and automobile industry, the sheet steels are the very important parts. In the recent years, laser welding technique had been used to weld the sheet steel part. The seam width between the two parts is usually less than 0.1mm. Because the error of the fixture fixed can’t be eliminated, the welding parts quality can be greatly affected. In order to improve the welding quality, the line structured light is employed in the vision monitoring system to plan the welding path before welding. In order to improve the weld precision, the vision system is located on Z axis of the computer numerical control (CNC) tool. The planar pattern is placed on the X-Y plane of the CNC tool, and the structured light is projected on the planar pattern. The vision system stay at three different positions along the Z axis of the CNC tool, and the camera shoot the image of the planar pattern at every position. Using the calculated the sub-pixel center line of the structure light, the world coordinate of the center light line can be calculated. Thus, the structured light plane can be calculated by fitting the structured light line. Experiment result shows the effective of the proposed method.

Shifts in Selective Pressures on Snake Phototransduction Genes Associated with Photoreceptor Transmutation and Dim-Light Ancestry.

PubMed

Schott, Ryan K; Van Nynatten, Alexander; Card, Daren C; Castoe, Todd A; S W Chang, Belinda

2018-06-01

The visual systems of snakes are heavily modified relative to other squamates, a condition often thought to reflect their fossorial origins. Further modifications are seen in caenophidian snakes, where evolutionary transitions between rod and cone photoreceptors, termed photoreceptor transmutations, have occurred in many lineages. Little previous work, however, has focused on the molecular evolutionary underpinnings of these morphological changes. To address this, we sequenced seven snake eye transcriptomes and utilized new whole-genome and targeted capture sequencing data. We used these data to analyze gene loss and shifts in selection pressures in phototransduction genes that may be associated with snake evolutionary origins and photoreceptor transmutation. We identified the surprising loss of rhodopsin kinase (GRK1), despite a low degree of gene loss overall and a lack of relaxed selection early during snake evolution. These results provide some of the first evolutionary genomic corroboration for a dim-light ancestor that lacks strong fossorial adaptations. Our results also indicate that snakes with photoreceptor transmutation experienced significantly different selection pressures from other reptiles. Significant positive selection was found primarily in cone-specific genes, but not rod-specific genes, contrary to our expectations. These results reveal potential molecular adaptations associated with photoreceptor transmutation and also highlight unappreciated functional differences between rod- and cone-specific phototransduction proteins. This intriguing example of snake visual system evolution illustrates how the underlying molecular components of a complex system can be reshaped in response to changing selection pressures.

Solar Demon: near real-time Flare, Dimming and EUV wave monitoring

NASA Astrophysics Data System (ADS)

Kraaikamp, Emil; Verbeeck, Cis

Dimmings and EUV waves have been observed routinely in EUV images since 1996. They are closely associated with coronal mass ejections (CMEs), and therefore provide useful information for early space weather alerts. On the one hand, automatic detection and characterization of dimmings and EUV waves can be used to gain better understanding of the underlying physical mechanisms. On the other hand, every dimming and EUV wave provides extra information on the associated front side CME, and can improve estimates of the geo-effectiveness and arrival time of the CME. Solar Demon has been designed to detect and characterize dimmings, EUV waves, as well as solar flares in near real-time on Solar Dynamics Observatory/Atmospheric Imaging Assembly (SDO/AIA) data. The detection modules are running continuously at the Royal Observatory of Belgium on both quick-look data, as well as synoptic science data. The output of Solar Demon can be accessed in near real-time on the Solar Demon website, and includes images, movies, light curves, and the numerical evolution of several parameters. Solar Demon is the result of collaboration between the FP7 projects AFFECTS and COMESEP. Flare detections of Solar Demon are integrated into the COMESEP alert system. Here we present the Solar Demon detection algorithms and their output. We will show several interesting flare, dimming and EUV wave events, and present general statistics of the detections made so far during solar cycle 24.

Exposure to dim artificial light at night increases REM sleep and awakenings in humans.

PubMed

Cho, Chul-Hyun; Lee, Heon-Jeong; Yoon, Ho-Kyoung; Kang, Seung-Gul; Bok, Ki-Nam; Jung, Ki-Young; Kim, Leen; Lee, Eun-Il

2016-01-01

Exposure to artificial light at night (ALAN) has become increasing common, especially in developed countries. We investigated the effect of dALAN exposure during sleep in healthy young male subjects. A total of 30 healthy young male volunteers from 21 to 29 years old were recruited for the study. They were randomly divided into two groups depending on light intensity (Group A: 5 lux and Group B: 10 lux). After a quality control process, 23 healthy subjects were included in the study (Group A: 11 subjects, Group B: 12 subjects). Subjects underwent an NPSG session with no light (Night 1) followed by an NPSG session randomly assigned to two different dim light conditions (5 or 10 lux, dom λ: 501.4 nm) for a whole night (Night 2). We found significant sleep structural differences between Nights 1 and 2, but no difference between Groups A and B. Exposure to dALAN during sleep was significantly associated with increased wake time after sleep onset (WASO; F = 7.273, p = 0.014), increased Stage N1 (F = 4.524, p = 0.045), decreased Stage N2 (F = 9.49, p = 0.006), increased Stage R (F = 6.698, p = 0.017) and non-significantly decreased REM density (F = 4.102, p = 0.056). We found that dALAN during sleep affects sleep structure. Exposure to dALAN during sleep increases the frequency of arousals, amount of shallow sleep and amount of REM sleep. This suggests adverse effects of dALAN during sleep on sleep quality and suggests the need to avoid exposure to dALAN during sleep.

Dim light at night increases depressive-like responses in male C3H/HeNHsd mice.

PubMed

Fonken, Laura K; Nelson, Randy J

2013-04-15

Daily patterns of light exposure have become increasingly variable since the widespread adoption of electrical lighting during the 20th century. Seasonal fluctuations in light exposure, shift-work, and transmeridian travel are all associated with alterations in mood. These studies implicate fluctuations in environmental lighting in the development of depressive disorders. Here we argue that exposure to light at night (LAN) may be causally linked to depression. Male C3H/HeNHsd mice, which produce nocturnal melatonin, were housed in either a standard light/dark (LD) cycle or exposed to nightly dim (5 lux) LAN (dLAN). After four weeks in lighting conditions mice underwent behavioral testing and hippocampal tissue was collected at the termination of the study for qPCR. Here were report that mice exposed to dLAN increase depressive-like responses in both a sucrose anhedonia and forced swim test. In contrast to findings in diurnal grass rats, dLAN mice perform comparably to mice housed under dark nights in a hippocampus-dependent learning and memory task. TNFα and IL1β gene expression do not differ between groups, demonstrating that changes in these pro-inflammatory cytokines do not mediate dLAN induced depressive-like responses in mice. BDNF expression is reduced in the hippocampus of mice exposed to dLAN. These results indicate that low levels of LAN can alter mood in mice. This study along with previous work implicates LAN as a potential factor contributing to depression. Further understanding of the mechanisms through which LAN contributes to changes in mood is important for characterizing and treating depressive disorders. Copyright © 2013 Elsevier B.V. All rights reserved.

Assessing the Dim Light Melatonin Onset in Adults with Autism Spectrum Disorder and No Comorbid Intellectual Disability.

PubMed

Baker, Emma K; Richdale, Amanda L; Hazi, Agnes; Prendergast, Luke A

2017-07-01

This study assessed melatonin levels and the dim light melatonin onset (DLMO) in adults with Autism Spectrum Disorder (ASD) and also investigated the relationships between melatonin and objectively measured sleep parameters. Sixteen adults with ASD (ASD-Only), 12 adults with ASD medicated for comorbid diagnoses of anxiety and/or depression (ASD-Med) and 32 controls participated in the study. Although, the timing of the DLMO did not differ between the two groups, advances and delays of the melatonin rhythm were observed in individual profiles. Overall mean melatonin levels were lower in the ASD-Med group compared to the two other groups. Lastly, greater increases in melatonin in the hour prior to sleep were associated with greater sleep efficiency in the ASD groups.

Effects of Dim Light at Night on Food Intake and Body Mass in Developing Mice.

PubMed

Cissé, Yasmine M; Peng, Juan; Nelson, Randy J

2017-01-01

Appropriately timed light is critical for circadian organization; exposure to dim light at night (dLAN) disrupts temporal organization of endogenous biological timing. Exposure to dLAN in adult mice is associated with elevated body mass and changes in metabolism putatively driven by voluntary changes in the time of food intake. We predicted that exposure of young mice to LAN could affect adult metabolic function. At 3 weeks (Experiment 1) or 5 weeks (Experiment 2) of age, mice were either maintained in standard light-dark (DARK) cycles or exposed to nightly dLAN (5 lux). In the first two experiments, food intake and locomotor activity were assessed after 4 weeks and a glucose tolerance test was administered after 6 weeks in experimental lighting conditions. In Experiment 3, tissues were collected around the clock at 6 h intervals to investigate rhythmic hepatic clock gene expression in mice exposed to dLAN from 3 or 5 weeks of age. Male and female mice exposed to dLAN beginning at 3 weeks of age displayed similar growth rates and body mass to DARK-reared offspring, despite increasing day-time food intake. Exposure to dLAN beginning at 5 weeks of age increased body mass and daytime food intake in male, but not female, mice. Consistent with the body mass phenotype, clock gene expression was unaltered in the liver. In contrast to adults, dLAN exposure during the development of the peripheral circadian system has sex- and development-dependent effects on body mass gain.

Increased visual sensitivity following periods of dim illumination.

PubMed

McKeown, Alex S; Kraft, Timothy W; Loop, Michael S

2015-02-19

We measured changes in the sensitivity of the human rod pathway by testing visual reaction times before and after light adaptation. We targeted a specific range of conditioning light intensities to see if a physiological adaptation recently discovered in mouse rods is observable at the perceptual level in humans. We also measured the noise spectrum of single mouse rods due to the importance of the signal-to-noise ratio in rod to rod bipolar cell signal transfer. Using the well-defined relationship between stimulus intensity and reaction time (Piéron's law), we measured the reaction times of eight human subjects (ages 24-66) to scotopic test flashes of a single intensity before and after the presentation of a 3-minute background. We also made recordings from single mouse rods and processed the cellular noise spectrum before and after similar conditioning exposures. Subject reaction times to a fixed-strength stimulus were fastest 5 seconds after conditioning background exposure (79% ± 1% of the preconditioning mean, in darkness) and were significantly faster for the first 12 seconds after background exposure (P < 0.01). During the period of increased rod sensitivity, the continuous noise spectrum of individual mouse rods was not significantly increased. A decrease in human reaction times to a dim flash after conditioning background exposure may originate in rod photoreceptors through a transient increase in the sensitivity of the phototransduction cascade. There is no accompanying increase in rod cellular noise, allowing for reliable transmission of larger rod signals after conditioning exposures and the observed increase in perceptual sensitivity. Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.

Flight control and landing precision in the nocturnal bee Megalopta is robust to large changes in light intensity.

PubMed

Baird, Emily; Fernandez, Diana C; Wcislo, William T; Warrant, Eric J

2015-01-01

Like their diurnal relatives, Megalopta genalis use visual information to control flight. Unlike their diurnal relatives, however, they do this at extremely low light intensities. Although Megalopta has developed optical specializations to increase visual sensitivity, theoretical studies suggest that this enhanced sensitivity does not enable them to capture enough light to use visual information to reliably control flight in the rainforest at night. It has been proposed that Megalopta gain extra sensitivity by summing visual information over time. While enhancing the reliability of vision, this strategy would decrease the accuracy with which they can detect image motion-a crucial cue for flight control. Here, we test this temporal summation hypothesis by investigating how Megalopta's flight control and landing precision is affected by light intensity and compare our findings with the results of similar experiments performed on the diurnal bumblebee Bombus terrestris, to explore the extent to which Megalopta's adaptations to dim light affect their precision. We find that, unlike Bombus, light intensity does not affect flight and landing precision in Megalopta. Overall, we find little evidence that Megalopta uses a temporal summation strategy in dim light, while we find strong support for the use of this strategy in Bombus.

Effects of Background Lighting Color and Movement Distance on Reaching Times Among Participants With Low Vision, Myopia, and Normal Vision.

PubMed

Chen, Chun-Fu; Huang, Kuo-Chen

2016-04-01

This study investigated the effects of target distance (30, 35, and 40 cm) and the color of background lighting (red, green, blue, and yellow) on the duration of movements made by participants with low vision, myopia, and normal vision while performing a reaching task; 48 students (21 women, 27 men; M age = 21.8 year, SD = 2.4) participated in the study. Participants reached for a target (a white LED light) whose vertical position varied randomly across trials, ranging in distance from 30 to 40 cm. Movement time was analyzed using a 3 (participant group) × [4 (color of background lighting) × 3 (movement distance)] mixed-design ANOVA model. Results indicated longer times for completing a reaching movement when: participants belonged to the low vision group; the target distance between the starting position and the target position was longer (40 cm); and the reaching movement occurred in the red-background lighting condition. These results are particularly relevant for situations in which a user is required to respond to a signal by reaching toward a button or an icon. © The Author(s) 2016.

Comparative studies on dimming capabilities of retrofit LED lamps

NASA Astrophysics Data System (ADS)

Ionescu, Ciprian; Vasile, Alexandru; Codreanu, Norocel; Negroiu, Rodica

2016-12-01

These days many variants for lighting systems are available on the market, and new solutions are about to emerge. Most of the new lamps are offered in form to be retrofitted to existing sockets and luminaires. In this paper, are presented some systematically investigations on different lamps as LEDs, Compact Fluorescent Lamps (CFLs), tungsten, and new available Cold Cathode Fluorescent Lamps (CCFLs), regarding the light level, dimming performances and also the resulting flicker and power distortion performances. The light level was expressed by the illuminance level, measured for all lamps in the same conditions, at the same distance and on the same surface represented by the photometer probe.

Is vision function related to physical functional ability in older adults?

PubMed

West, Catherine G; Gildengorin, Ginny; Haegerstrom-Portnoy, Gunilla; Schneck, Marilyn E; Lott, Lori; Brabyn, John A

2002-01-01

To assess the relationship between a broad range of vision functions and measures of physical performance in older adults. Cross-sectional study. Population-based cohort of community-dwelling older adults, subset of an on-going longitudinal study. Seven hundred eighty-two adults aged 55 and older (65% of living eligible subjects) had subjective health measures and objective physical performance evaluated in 1989/91 and again in 1993/95 and a battery of vision functions tested in 1993/95. Comprehensive battery of vision tests (visual acuity, contrast sensitivity, effects of illumination level, contrast and glare on acuity, visual fields with and without attentional load, color vision, temporal sensitivity, and the impact of dimming light on walking ability) and physical function measures (self-reported mobility limitations and observed measures of walking, rising from a chair and tandem balance). The failure rate for all vision functions and physical performance measures increased exponentially with age. Standard high-contrast visual acuity and standard visual fields showed the lowest failure rates. Nonstandard vision tests showed much higher failure rates. Poor performance on many individual vision functions was significantly associated with particular individual measures of physical performance. Using constructed combination vision variables, significant associations were found between spatial vision, field integrity, binocularity and/or adaptation, and each of the functional outcomes. Vision functions other than standard visual acuity may affect day-to-day functioning of older adults. Additional studies of these other aspects of vision and how they can be treated or rehabilitated are needed to determine whether these aspects play a role in strategies for reducing disability in older adults.

Nocturnal light environments and species ecology: implications for nocturnal color vision in forests.

PubMed

Veilleux, Carrie C; Cummings, Molly E

2012-12-01

Although variation in the color of light in terrestrial diurnal and twilight environments has been well documented, relatively little work has examined the color of light in nocturnal habitats. Understanding the range and sources of variation in nocturnal light environments has important implications for nocturnal vision, particularly following recent discoveries of nocturnal color vision. In this study, we measured nocturnal irradiance in a dry forest/woodland and a rainforest in Madagascar over 34 nights. We found that a simple linear model including the additive effects of lunar altitude, lunar phase and canopy openness successfully predicted total irradiance flux measurements across 242 clear sky measurements (r=0.85, P<0.0001). However, the relationship between these variables and spectral irradiance was more complex, as interactions between lunar altitude, lunar phase and canopy openness were also important predictors of spectral variation. Further, in contrast to diurnal conditions, nocturnal forests and woodlands share a yellow-green-dominant light environment with peak flux at 560 nm. To explore how nocturnal light environments influence nocturnal vision, we compared photoreceptor spectral tuning, habitat preference and diet in 32 nocturnal mammals. In many species, long-wavelength-sensitive cone spectral sensitivity matched the peak flux present in nocturnal forests and woodlands, suggesting a possible adaptation to maximize photon absorption at night. Further, controlling for phylogeny, we found that fruit/flower consumption significantly predicted short-wavelength-sensitive cone spectral tuning in nocturnal mammals (P=0.002). These results suggest that variation in nocturnal light environments and species ecology together influence cone spectral tuning and color vision in nocturnal mammals.

Enriching lighting design.

PubMed

Brawley, Elizabeth C

2009-01-01

Good lighting is perhaps the most important and least understood element in designing healthcare environments. Both physically and mentally challenged individuals become more vulnerable and dependent on their environment to compensate for sensory impairments, including dimming eyesight, which interferes to some degree with daily activities as well as social and leisure activities - the things that provide emotional and social well-being. Too few building designs today result in lighting that meets the needs of these individuals, regardless of age. Typical lighting in most care environments is inadequate to meet lighting needs affecting both vision and the photobiological (non-visual) needs of synchronization of circadian rhythm, which impacts sleep and depression. Well-designed lighting is one of the most important design elements that will support an individual's ability to perform normal daily activities and decrease the level of disability associated with these impairments. Daylight contains the spectrum to which the circadian clock is most sensitive and provides higher light levels during the day. Easily accessible outdoor gardens encourage individuals outside, providing the necessary regular exposure to direct bright light that sunlight provides. The combination good interior lighting and regular daylight exposure contributes to regaining and maintaining an active and fulfilling lifestyle - greatly improving quality of life.

Statistical Study of Solar Dimmings Using CoDiT

DOE Office of Scientific and Technical Information (OSTI.GOV)

Krista, Larisza D.; Reinard, Alysha A., E-mail: larisza.krista@noaa.gov

2017-04-10

We present the results from analyzing the physical and morphological properties of 154 dimmings (transient coronal holes) and the associated flares and coronal mass ejections (CMEs). Each dimming in our 2013 catalog was processed with the semi-automated Coronal Dimming Tracker using Solar Dynamics Observatory AIA 193 Å observations and HMI magnetograms. Instead of the typically used difference images, we used our coronal hole detection algorithm to detect transient dark regions “directly” in extreme ultraviolet (EUV) images. This allowed us to study dimmings as the footpoints of CMEs—in contrast with the larger, diffuse dimmings seen in difference images that represent themore » projected view of the rising, expanding plasma. Studying the footpoint-dimming morphology allowed us to better understand the CME structure in the low corona. While comparing the physical properties of dimmings, flares, and CMEs we were also able to identify relationships between the different parts of this complex eruptive phenomenon. We found that larger dimmings are longer-lived, suggesting that it takes longer to “close down” large open magnetic regions. Also, during their growth phase, smaller dimmings acquire a higher magnetic flux imbalance (i. e., become more unipolar) than larger dimmings. Furthermore, we found that the EUV intensity of dimmings (indicative of local electron density) correlates with how much plasma was removed and how energetic the eruption was. Studying the morphology of dimmings (single, double, fragmented) also helped us identify different configurations of the quasi-open magnetic field.« less

Effects of four dim vs high intensity red color light regimens on growth performance and welfare of broilers.

PubMed

Senaratna, D; Samarakone, T S; Gunawardena, W W D A

2018-01-01

Broilers show clear preference towards red color light (RL). However setting of an optimum light intensity is difficult since dim intensities that favor growth reduce welfare. This experiment was conducted to test the most effective RL intensity regimen (Dim [5 lux; DI] vs high [320 lux; HI]) in combination applied at different growth stages that favors for both performance and welfare. Complete randomize design was adopted with 6 replicates. Treatments were; T1 = early DI (8-21 d)+latter HI (22-35 d); T2 = early DI (8-28 d)+latter HI (29-35 d), T3 = early HI (8-21 d)+latter DI (22-35 d), T4 = early HI (8-28 d)+latter DI (29-35 d) and T5 = control (white light; WT) (8-35 d) at medium intensity (20 lux). Body weight (BW), weight gain (WG), water/feed intake and ratio, feed conversion ratios (FCR) were assessed. Common behaviours (15) were recorded by scan sampling method. Lameness, foot pad dermatitis, breast blisters, hock burning damage were assessed as welfare parameters. Fear reactions were tested using Tonic Immobility Test. Ocular and carcass evaluations were done. Meat and tibiae were analyzed for fat and bone ash respectively. On 35 d, the highest BW (2,155.72±176 g), WG (1,967.78±174 g) were recorded by T2 compared to WT (BW WT = 1,878.22±155, WG WT = 1,691.83±160). But, application of RL, either DI, or HI during early/latter stage had no significant effect on FCR. Under HI, birds showed much higher active behaviours. DI encourages eating. Though LI changed from DI to HI, same trend could be seen even under HI. The highest leg strength (218.5±120 s) was recorded by T2. The lowest leg strength (64.58±33 s) and the highest ocular weight (2.48±1 g) were recorded by T1. Significantly (p<0.05) the highest skin weight (162.17±6 g) but the lowest fat% in meat (13.03%±5%) was recorded by T2. Early exposure to DI-RL up to 28 days followed by exposure to HI-RL is the most favorable lighting regimen for optimizing production, better welfare of broilers and

Effects of four dim vs high intensity red color light regimens on growth performance and welfare of broilers

PubMed Central

Samarakone, T. S.; Gunawardena, W. W. D. A.

2018-01-01

Objective Broilers show clear preference towards red color light (RL). However setting of an optimum light intensity is difficult since dim intensities that favor growth reduce welfare. This experiment was conducted to test the most effective RL intensity regimen (Dim [5 lux; DI] vs high [320 lux; HI]) in combination applied at different growth stages that favors for both performance and welfare. Methods Complete randomize design was adopted with 6 replicates. Treatments were; T1 = early DI (8–21 d)+latter HI (22–35 d); T2 = early DI (8–28 d)+latter HI (29–35 d), T3 = early HI (8–21 d)+latter DI (22–35 d), T4 = early HI (8–28 d)+latter DI (29–35 d) and T5 = control (white light; WT) (8–35 d) at medium intensity (20 lux). Body weight (BW), weight gain (WG), water/feed intake and ratio, feed conversion ratios (FCR) were assessed. Common behaviours (15) were recorded by scan sampling method. Lameness, foot pad dermatitis, breast blisters, hock burning damage were assessed as welfare parameters. Fear reactions were tested using Tonic Immobility Test. Ocular and carcass evaluations were done. Meat and tibiae were analyzed for fat and bone ash respectively. Results On 35 d, the highest BW (2,155.72±176 g), WG (1,967.78±174 g) were recorded by T2 compared to WT (BWWT = 1,878.22±155, WGWT = 1,691.83±160). But, application of RL, either DI, or HI during early/latter stage had no significant effect on FCR. Under HI, birds showed much higher active behaviours. DI encourages eating. Though LI changed from DI to HI, same trend could be seen even under HI. The highest leg strength (218.5±120 s) was recorded by T2. The lowest leg strength (64.58±33 s) and the highest ocular weight (2.48±1 g) were recorded by T1. Significantly (p<0.05) the highest skin weight (162.17±6 g) but the lowest fat% in meat (13.03%±5%) was recorded by T2. Conclusion Early exposure to DI-RL up to 28 days followed by exposure to HI-RL is the most favorable lighting regimen for

The effect of dim light at night on cerebral hemodynamic oscillations during sleep: A near-infrared spectroscopy study.

PubMed

Kim, Tae-Joon; Lee, Byeong Uk; Sunwoo, Jun-Sang; Byun, Jung-Ick; Moon, Jangsup; Lee, Soon-Tae; Jung, Keun-Hwa; Chu, Kon; Kim, Manho; Lim, Jong-Min; Lee, Eunil; Lee, Sang Kun; Jung, Ki-Young

2017-01-01

Recent studies have reported that dim light at night (dLAN) is associated with risks of cardiovascular complications, such as hypertension and carotid atherosclerosis; however, little is known about the underlying mechanism. Here, we evaluated the effect of dLAN on the cerebrovascular system by analyzing cerebral hemodynamic oscillations using near-infrared spectroscopy (NIRS). Fourteen healthy male subjects underwent polysomnography coupled with cerebral NIRS. The data collected during sleep with dim light (10 lux) were compared with those collected during sleep under the control dark conditions for the sleep structure, cerebral hemodynamic oscillations, heart rate variability (HRV), and their electroencephalographic (EEG) power spectrum. Power spectral analysis was applied to oxy-hemoglobin concentrations calculated from the NIRS signal. Spectral densities over endothelial very-low-frequency oscillations (VLFOs) (0.003-0.02 Hz), neurogenic VLFOs (0.02-0.04 Hz), myogenic low-frequency oscillations (LFOs) (0.04-0.15 Hz), and total LFOs (0.003-0.15 Hz) were obtained for each sleep stage. The polysomnographic data revealed an increase in the N2 stage under the dLAN conditions. The spectral analysis of cerebral hemodynamics showed that the total LFOs increased significantly during slow-wave sleep (SWS) and decreased during rapid eye movement (REM) sleep. Specifically, endothelial (median of normalized value, 0.46 vs. 0.72, p = 0.019) and neurogenic (median, 0.58 vs. 0.84, p = 0.019) VLFOs were enhanced during SWS, whereas endothelial VLFOs (median, 1.93 vs. 1.47, p = 0.030) were attenuated during REM sleep. HRV analysis exhibited altered spectral densities during SWS induced by dLAN, including an increase in very-low-frequency and decreases in low-frequency and high-frequency ranges. In the EEG power spectral analysis, no significant difference was detected between the control and dLAN conditions. In conclusion, dLAN can disturb cerebral hemodynamics via the

Circadian phase-shifting effects of a laboratory environment: a clinical trial with bright and dim light.

PubMed

Youngstedt, Shawn D; Kripke, Daniel F; Elliott, Jeffrey A; Rex, Katharine M

2005-09-09

Our aims were to examine the influence of different bright light schedules on mood, sleep, and circadian organization in older adults (n = 60, ages 60-79 years) with insomnia and/or depression, contrasting with responses of young, healthy controls (n = 30, ages 20-40 years). Volunteers were assessed for one week in their home environments. Urine was collected over two 24-hour periods to establish baseline acrophase of 6-sulphatoxymelatonin (aMT6s) excretion. Immediately following home recording, volunteers spent five nights and four days in the laboratory. Sleep periods were fixed at eight hours in darkness, consistent with the volunteers' usual sleep periods. Volunteers were randomly assigned to one of three light treatments (four hours per day) within the wake period: (A) two hours of 3,000 lux at 1-3 hours and 13-15 hours after arising; (B) four hours of 3,000 lux at 6-10 hours after arising; (C) four hours of dim placebo light at 6-10 hours after arising. Lighting was 50 lux during the remainder of wakefulness. The resulting aMT6s acrophase was determined during the final 30 hours in the laboratory. Neither mood nor total melatonin excretion differed significantly by treatment. For the three light treatments, significant and similar phase-response plots were found, indicating that the shift in aMT6s acrophase was dependent upon the circadian time of treatment. The changes in circadian timing were not significantly correlated to changes in sleep or mood. The trial failed to demonstrate photoperiodic effects. The results suggest that even low levels of illumination and/or fixed timing of behavior had significant phase-shifting effects.

Dimming of metal halide lamps

NASA Astrophysics Data System (ADS)

Schurer, Kees

1994-03-01

We ran some tests on the effect of dimming of metal halide (MH) lamps upon the stability and the spectral quality of the light output. Lamps used were a new Philips lamp HPI-T 250W, a similar Philips lamp with a few thousand burning hours and a new Osram lamp HQI-T 250W/D. The ballast was a BBC type DJ 250/2KS, the starter a BAS TORGI type MZN 250 SE and the dimmer an Elstrom Control System type ERHQ-T 250. Power was derived from a Philips stabilizer, type PE 1602. Lamp output was monitored with a PAR meter. Spectra were taken at 100% and at 50% output as measured with the PAR meter. Lamps were allowed to stabilize at any setting for 30 minutes before measurements were made. Lamp manufacturers advise against dimming for fear of poor stability and intolerable changes of the spectrum. However, none of the lamps showed a decrease in stability, no flicker or wandering of the discharge, and the changes of the spectrum were not negligible, but certainly not dramatic. Lamps of either manufacture retain their white color, relative peak heights of spectral lines did shift, but no gaps in the spectrum occurred. Spectra taken at 50% with 30 minutes intervals coincided. Differences between the new and the older Philips lamp were noticeable, but not really significant.

Dimming of metal halide lamps

NASA Technical Reports Server (NTRS)

Schurer, Kees

1994-01-01

We ran some tests on the effect of dimming of metal halide (MH) lamps upon the stability and the spectral quality of the light output. Lamps used were a new Philips lamp HPI-T 250W, a similar Philips lamp with a few thousand burning hours and a new Osram lamp HQI-T 250W/D. The ballast was a BBC type DJ 250/2KS, the starter a BAS TORGI type MZN 250 SE and the dimmer an Elstrom Control System type ERHQ-T 250. Power was derived from a Philips stabilizer, type PE 1602. Lamp output was monitored with a PAR meter. Spectra were taken at 100% and at 50% output as measured with the PAR meter. Lamps were allowed to stabilize at any setting for 30 minutes before measurements were made. Lamp manufacturers advise against dimming for fear of poor stability and intolerable changes of the spectrum. However, none of the lamps showed a decrease in stability, no flicker or wandering of the discharge, and the changes of the spectrum were not negligible, but certainly not dramatic. Lamps of either manufacture retain their white color, relative peak heights of spectral lines did shift, but no gaps in the spectrum occurred. Spectra taken at 50% with 30 minutes intervals coincided. Differences between the new and the older Philips lamp were noticeable, but not really significant.

LED light design method for high contrast and uniform illumination imaging in machine vision.

PubMed

Wu, Xiaojun; Gao, Guangming

2018-03-01

In machine vision, illumination is very critical to determine the complexity of the inspection algorithms. Proper lights can obtain clear and sharp images with the highest contrast and low noise between the interested object and the background, which is conducive to the target being located, measured, or inspected. Contrary to the empirically based trial-and-error convention to select the off-the-shelf LED light in machine vision, an optimization algorithm for LED light design is proposed in this paper. It is composed of the contrast optimization modeling and the uniform illumination technology for non-normal incidence (UINI). The contrast optimization model is built based on the surface reflection characteristics, e.g., the roughness, the reflective index, and light direction, etc., to maximize the contrast between the features of interest and the background. The UINI can keep the uniformity of the optimized lighting by the contrast optimization model. The simulation and experimental results demonstrate that the optimization algorithm is effective and suitable to produce images with the highest contrast and uniformity, which is very inspirational to the design of LED illumination systems in machine vision.

Flight control and landing precision in the nocturnal bee Megalopta is robust to large changes in light intensity

PubMed Central

Baird, Emily; Fernandez, Diana C.; Wcislo, William T.; Warrant, Eric J.

2015-01-01

Like their diurnal relatives, Megalopta genalis use visual information to control flight. Unlike their diurnal relatives, however, they do this at extremely low light intensities. Although Megalopta has developed optical specializations to increase visual sensitivity, theoretical studies suggest that this enhanced sensitivity does not enable them to capture enough light to use visual information to reliably control flight in the rainforest at night. It has been proposed that Megalopta gain extra sensitivity by summing visual information over time. While enhancing the reliability of vision, this strategy would decrease the accuracy with which they can detect image motion—a crucial cue for flight control. Here, we test this temporal summation hypothesis by investigating how Megalopta's flight control and landing precision is affected by light intensity and compare our findings with the results of similar experiments performed on the diurnal bumblebee Bombus terrestris, to explore the extent to which Megalopta's adaptations to dim light affect their precision. We find that, unlike Bombus, light intensity does not affect flight and landing precision in Megalopta. Overall, we find little evidence that Megalopta uses a temporal summation strategy in dim light, while we find strong support for the use of this strategy in Bombus. PMID:26578977

Differences in vision between clinic and home and the effect of lighting in older adults with and without glaucoma

PubMed Central

Bhorade, Anjali M.; Perlmutter, Monica S.; Wilson, Brad; Kambarian, Jamie; Chang, Sidney; Pekmezci, Melike; Gordon, Mae

2015-01-01

Importance Patients often report greater visual difficulties at home than expected from vision testing in the clinic. Such discordance may be due to worse vision in the home than measured in clinic. Objective To compare vision measured between clinic and home and evaluate factors, including lighting, associated with these differences. Design Cross-sectional, between years 2005–2009. Setting Participants recruited from the Glaucoma and Comprehensive Eye Clinics at Washington University, St. Louis, MO underwent a clinic and home visit. Participants 126 glaucoma and 49 non-glaucoma patients, ages 55–90 years, consecutively recruited and met inclusion criteria for this report; 166 eligible patients refused participation. Exposure Participants underwent a clinic and home visit randomized to order of completion. At each visit, masked and certified examiners measured binocular distance visual acuity (DVA) with a non-backlit chart, near visual acuity (NVA), contrast sensitivity (CS), CS with glare, and lighting. Main Outcome Measure Differences in vision between clinic and home. Results Mean scores for all vision tests were significantly better in the clinic than home for glaucoma and non-glaucoma patients (p<0.05, matched pair t-tests). For DVA, 29% of glaucoma participants read ≥2 lines better in clinic than home and 39% with advanced glaucoma read ≥3 lines better. For the entire sample, 21% of participants read ≥2 lines better in clinic than home for NVA and 49% read ≥2 triplets better in clinic for CS with glare. Lighting was the most significant factor associated with differences in vision between clinic and home for DVA, NVA, and CS with glare testing (p<0.05 multiple regression model). Median home lighting was 4.3 times and 2.8 times lower than clinic lighting in areas tested for DVA and NVA, respectively. Home lighting was below that recommended in ≥ 85% of participants. Conclusions and Relevance Vision measured in the clinic is generally better than

Circadian phase assessment by ambulatory monitoring in humans: correlation with dim light melatonin onset.

PubMed

Bonmati-Carrion, M A; Middleton, B; Revell, V; Skene, D J; Rol, M A; Madrid, J A

2014-02-01

The increased prevalence of circadian disruptions due to abnormal coupling between internal and external time makes the detection of circadian phase in humans by ambulatory recordings a compelling need. Here, we propose an accurate practical procedure to estimate circadian phase with the least possible burden for the subject, that is, without the restraints of a constant routine protocol or laboratory techniques such as melatonin quantification, both of which are standard procedures. In this validation study, subjects (N = 13) wore ambulatory monitoring devices, kept daily sleep diaries and went about their daily routine for 10 days. The devices measured skin temperature at wrist level (WT), motor activity and body position on the arm, and light exposure by means of a sensor placed on the chest. Dim light melatonin onset (DLMO) was used to compare and evaluate the accuracy of the ambulatory variables in assessing circadian phase. An evening increase in WT: WTOnset (WTOn) and "WT increase onset" (WTiO) was found to anticipate the evening increase in melatonin, while decreases in motor activity (Activity Offset or AcOff), body position (Position Offset (POff)), integrative TAP (a combination of WT, activity and body position) (TAPOffset or TAPOff) and an increase in declared sleep propensity were phase delayed with respect to DLMO. The phase markers obtained from subjective sleep (R = 0.811), WT (R = 0.756) and the composite variable TAP (R = 0.720) were highly and significantly correlated with DLMO. The findings strongly support a new method to calculate circadian phase based on WT (WTiO) that accurately predicts and shows a temporal association with DLMO. WTiO is especially recommended due to its simplicity and applicability to clinical use under conditions where knowing endogenous circadian phase is important, such as in cancer chronotherapy and light therapy.

Termination of short term melatonin treatment in children with delayed Dim Light Melatonin Onset: effects on sleep, health, behavior problems, and parenting stress.

PubMed

van Maanen, Annette; Meijer, Anne Marie; Smits, Marcel G; Oort, Frans J

2011-10-01

To investigate the effects of termination of short term melatonin treatment on sleep, health, behavior, and parenting stress in children with delayed Dim Light Melatonin Onset. Forty-one children (24 boys, 17 girls; mean age=9.43 years) entered melatonin treatment for 3 weeks and then discontinued treatment by first taking a half dose for 1 week and then stopping completely for another week. Sleep was measured with sleep diaries filled in by parents and with actometers worn by children. Analyses were conducted with linear mixed models. Sleep latency was longer during the stop week compared to the treatment weeks. Sleep start was later and actual sleep time was shorter during the half dose and stop weeks compared to the treatment weeks. Sleep efficiency deteriorated in the stop week. Dim Light Melatonin Onset was earlier after treatment, but this effect disappeared after the stop week. In addition to the effects on sleep, results from questionnaires completed by parents showed that melatonin treatment also had positive effects on children's health and behavior problems and parenting stress. While health deteriorated after treatment discontinuation, the effects on behavior problems and parenting stress remained. Behavior problems at baseline did not influence the effect of melatonin treatment. This study showed that complete termination of treatment after 4 weeks of melatonin use was too early. However, clinicians may advise a lower dose after a successful treatment trial of several weeks. Copyright © 2011 Elsevier B.V. All rights reserved.

Characterization and effect of light on the plasma membrane H(+) -ATPase of bean leaves

NASA Technical Reports Server (NTRS)

Linnemeyer, P. A.; Van Volkenburgh, E.; Cleland, R. E.

1990-01-01

Proton excretion from bean (Phaseolus vulgaris L.) leaf cells is increased by bright white light. To test whether this could be due, at least in part, to an increase in plasma membrane (PM) ATPase activity, PM vesicles were isolated from primary leaves by phase partitioning and used to characterize PM ATPase activity and changes in response to light. ATPase activity was characterized as magnesium ion dependent, vanadate sensitive, and slightly stimulated by potassium chloride. The pH optimum was 6.5, the Km was approximately 0.30 millimolar ATP, and the activity was about 60% latent. PM vesicles were prepared from leaves of plants grown for 11 days in dim red light (growing slowly) or grown for 10 days in dim red light and then transferred to bright white-light for 1 day (growing rapidly). For both light treatments, ATPase specific activity was approximately 600 to 700 nanomoles per milligram protein per minute, and the latency, Km, and sensitivity to potassium chloride were also similar. PM vesicles from plants grown in complete darkness, however, exhibited a twofold greater specific activity. We conclude that the promotion of leaf growth and proton excretion by bright white light is not due to an increase in ATPase specific activity. Light does influence ATPase activity, however; both dim red light and bright white light decreased the ATPase specific activity by nearly 50% as compared with dark-grown leaves.

Human vision is attuned to the diffuseness of natural light

PubMed Central

Morgenstern, Yaniv; Geisler, Wilson S.; Murray, Richard F.

2014-01-01

All images are highly ambiguous, and to perceive 3-D scenes, the human visual system relies on assumptions about what lighting conditions are most probable. Here we show that human observers' assumptions about lighting diffuseness are well matched to the diffuseness of lighting in real-world scenes. We use a novel multidirectional photometer to measure lighting in hundreds of environments, and we find that the diffuseness of natural lighting falls in the same range as previous psychophysical estimates of the visual system's assumptions about diffuseness. We also find that natural lighting is typically directional enough to override human observers' assumption that light comes from above. Furthermore, we find that, although human performance on some tasks is worse in diffuse light, this can be largely accounted for by intrinsic task difficulty. These findings suggest that human vision is attuned to the diffuseness levels of natural lighting conditions. PMID:25139864

Differential Light-induced Responses in Sectorial Inherited Retinal Degeneration*

PubMed Central

Ramon, Eva; Cordomí, Arnau; Aguilà, Mònica; Srinivasan, Sundaramoorthy; Dong, Xiaoyun; Moore, Anthony T.; Webster, Andrew R.; Cheetham, Michael E.; Garriga, Pere

2014-01-01

Retinitis pigmentosa (RP) is a group of genetically and clinically heterogeneous inherited degenerative retinopathies caused by abnormalities of photoreceptors or retinal pigment epithelium in the retina leading to progressive sight loss. Rhodopsin is the prototypical G-protein-coupled receptor located in the vertebrate retina and is responsible for dim light vision. Here, novel M39R and N55K variants were identified as causing an intriguing sector phenotype of RP in affected patients, with selective degeneration in the inferior retina. To gain insights into the molecular aspects associated with this sector RP phenotype, whose molecular mechanism remains elusive, the mutations were constructed by site-directed mutagenesis, expressed in heterologous systems, and studied by biochemical, spectroscopic, and functional assays. M39R and N55K opsins had variable degrees of chromophore regeneration when compared with WT opsin but showed no gross structural misfolding or altered trafficking. M39R showed a faster rate for transducin activation than WT rhodopsin with a faster metarhodopsinII decay, whereas N55K presented a reduced activation rate and an altered photobleaching pattern. N55K also showed an altered retinal release from the opsin binding pocket upon light exposure, affecting its optimal functional response. Our data suggest that these sector RP mutations cause different protein phenotypes that may be related to their different clinical progression. Overall, these findings illuminate the molecular mechanisms of sector RP associated with rhodopsin mutations. PMID:25359768

Image-classification-based global dimming algorithm for LED backlights in LCDs

NASA Astrophysics Data System (ADS)

Qibin, Feng; Huijie, He; Dong, Han; Lei, Zhang; Guoqiang, Lv

2015-07-01

Backlight dimming can help LCDs reduce power consumption and improve CR. With fixed parameters, dimming algorithm cannot achieve satisfied effects for all kinds of images. The paper introduces an image-classification-based global dimming algorithm. The proposed classification method especially for backlight dimming is based on luminance and CR of input images. The parameters for backlight dimming level and pixel compensation are adaptive with image classifications. The simulation results show that the classification based dimming algorithm presents 86.13% power reduction improvement compared with dimming without classification, with almost same display quality. The prototype is developed. There are no perceived distortions when playing videos. The practical average power reduction of the prototype TV is 18.72%, compared with common TV without dimming.

The effects of prior light history on the suppression of melatonin by light in humans

PubMed Central

Hébert, Marc; Martin, Stacia K.; Lee, Clara; Eastman, Charmane I.

2014-01-01

We investigated the impact of light exposure history on light sensitivity in humans, as assessed by the magnitude of the suppression of melatonin secretion by nocturnal light. The hypothesis was that following a week of increased daytime bright-light exposure, subjects would become less sensitive to light, and that after a week of restriction to dimmer light they would become more sensitive. During the bright week, subjects (n = 12) obtained 4.3 ± 0.4 hr of bright light per day (by going outside and using light boxes indoors). During the dim week, they wore dark goggles (about 2% light transmission) when outside during daylight and spent 1.4 ± 0.9 hr per day outside. Saliva samples were obtained every 30 min for 7 hr in dim light (<15 lux) on two consecutive nights (baseline and test night) at the end of each week. On the test night, 500 lux was presented for 3 hr in the middle of the collection period to suppress melatonin. There was significantly more suppression after the dim week compared with after the bright week (to 53 versus 41% of the baseline night values, P < 0.05). However, there were large individual differences, and the difference between the bright and dim weeks was most pronounced in seven of the 12 subjects. Possible reasons for these individual differences are discussed, including the possibility that 1 wk was not long enough to change light sensitivity in some subjects. In conclusion, this study suggests that the circadian system’s sensitivity to light can be affected by a recent change in light history. PMID:12390501

Lipid-based oral delivery systems for skin deposition of a potential chemopreventive DIM derivative: characterization and evaluation.

PubMed

Boakye, Cedar H A; Patel, Ketan; Patel, Apurva R; Faria, Henrique A M; Zucolotto, Valtencir; Safe, Stephen; Singh, Mandip

2016-10-01

The objective of this study was to explore the oral route as a viable potential for the skin deposition of a novel diindolylmethane derivative (DIM-D) for chemoprevention activity. Various lipid-based oral delivery systems were optimized and compared for enhancing DIM-D's oral bioavailability and skin deposition. Preformulation studies were performed to evaluate the log P and solubility of DIM-D. Microsomal metabolism, P-glycoprotein efflux, and caco-2 monolayer permeability of DIM-D were determined. Comparative evaluation of the oral absorption and skin deposition of DIM-D-loaded various lipid-based formulations was performed in rats. DIM-D showed pH-dependent solubility and a high log P value. It was not a strong substrate of microsomal degradation and P-glycoprotein. SMEDDs comprised of medium chain triglycerides, monoglycerides, and kolliphor-HS15 (36.70 ± 0.42 nm). SNEDDs comprised of long chain triglycerides, cremophor RH40, labrasol, and TPGS (84.00 ± 14.14 nm). Nanostructured lipid carriers (NLC) consisted of compritol, miglyol, and surfactants (116.50 ± 2.12 nm). The blank formulations all showed >70 % cell viability in caco-2 cells. Differential Scanning Calorimetry confirmed the amorphization of DIM-D within the lipid matrices while Atomic Force Microscopy showed particle size distribution similar to the dynamic light scattering data. DIM-D also showed reduced permeation across caco-2 monolayer that was enhanced (p < 0.05) by SNEDDs in comparison to SMEDDs and NLC. Fabsolute for DIM-D SNEDDs, SMEDDs, and NLC was 0.14, 0.04, and 0.007, respectively. SNEDDs caused 53.90, 11.32, and 15.08-fold more skin deposition of DIM-D than the free drug, SMEDDs, and NLC, respectively, at 2 h following oral administration and shows a viable potential for use in skin cancer chemoprevention. Graphical Abstract ᅟ.

Bright-light mask treatment of delayed sleep phase syndrome.

PubMed

Cole, Roger J; Smith, Julian S; Alcalá, Yvonne C; Elliott, Jeffrey A; Kripke, Daniel F

2002-02-01

We treated delayed sleep phase syndrome (DSPS) with an illuminated mask that provides light through closed eyelids during sleep. Volunteers received either bright white light (2,700 lux, n = 28) or dim red light placebo (0.1 lux, n = 26) for 26 days at home. Mask lights were turned on (< 0.01 lux) 4 h before arising, ramped up for 1 h, and remained on at full brightness until arising. Volunteers also attempted to systematically advance sleep time, avoid naps, and avoid evening bright light. The light mask was well tolerated and produced little sleep disturbance. The acrophase of urinary 6-sulphatoxymelatonin (6-SMT) excretion advanced significantly from baseline in the bright group (p < 0.0006) and not in the dim group, but final phases were not significantly earlier in the bright group (ANCOVA ns). Bright treatment did produce significantly earlier phases, however, among volunteers whose baseline 6-SMT acrophase was later than the median of 0602 h (bright shift: 0732-0554 h, p < 0.0009; dim shift: 0746-0717 h, ns; ANCOVA p = 0.03). In this subgroup, sleep onset advanced significantly only with bright but not dim treatment (sleep onset shift: bright 0306-0145 h, p < 0.0002; dim 0229-0211 h, ns; ANCOVA p < .05). Despite equal expectations at baseline, participants rated bright treatment as more effective than dim treatment (p < 0.04). We conclude that bright-light mask treatment advances circadian phase and provides clinical benefit in DSPS individuals whose initial circadian delay is relatively severe.

Vision in laboratory rodents-Tools to measure it and implications for behavioral research.

PubMed

Leinonen, Henri; Tanila, Heikki

2017-07-29

Mice and rats are nocturnal mammals and their vision is specialized for detection of motion and contrast in dim light conditions. These species possess a large proportion of UV-sensitive cones in their retinas and the majority of their optic nerve axons target superior colliculus rather than visual cortex. Therefore, it was a widely held belief that laboratory rodents hardly utilize vision during day-time behavior. This dogma is being questioned as accumulating evidence suggests that laboratory rodents are able to perform complex visual functions, such as perceiving subjective contours, and that declined vision may affect their performance in many behavioral tasks. For instance, genetic engineering may have unexpected consequences on vision as mouse models of Alzheimer's and Huntington's diseases have declined visual function. Rodent vision can be tested in numerous ways using operant training or reflex-based behavioral tasks, or alternatively using electrophysiological recordings. In this article, we will first provide a summary of visual system and explain its characteristics unique to rodents. Then, we present well-established techniques to test rodent vision, with an emphasis on pattern vision: visual water test, optomotor reflex test, pattern electroretinography and pattern visual evoked potentials. Finally, we highlight the importance of visual phenotyping in rodents. As the number of genetically engineered rodent models and volume of behavioral testing increase simultaneously, the possibility of visual dysfunctions needs to be addressed. Neglect in this matter potentially leads to crude biases in the field of neuroscience and beyond. Copyright © 2017 Elsevier B.V. All rights reserved.

Human phase response curve to a 1 h pulse of bright white light.

PubMed

St Hilaire, Melissa A; Gooley, Joshua J; Khalsa, Sat Bir S; Kronauer, Richard E; Czeisler, Charles A; Lockley, Steven W

2012-07-01

The phase resetting response of the human circadian pacemaker to light depends on the timing of exposure and is described by a phase response curve (PRC). The current study aimed to construct a PRC for a 1 h exposure to bright white light (∼8000 lux) and to compare this PRC to a <3 lux dim background light PRC. These data were also compared to a previously completed 6.7 h bright white light PRC and a <15 lux dim background light PRC constructed under similar conditions. Participants were randomized for exposure to 1 h of either bright white light (n=18) or <3 lux dim background light (n=18) scheduled at 1 of 18 circadian phases. Participants completed constant routine (CR) procedures in dim light (<3 lux) before and after the light exposure to assess circadian phase. Phase shifts were calculated as the difference in timing of dim light melatonin onset (DLMO) during pre- and post-stimulus CRs. Exposure to 1 h of bright white light induced a Type 1 PRC with a fitted peak-to-trough amplitude of 2.20 h. No discernible PRC was observed in the <3 lux dim background light PRC. The fitted peak-to-trough amplitude of the 1 h bright light PRC was ∼40% of that for the 6.7 h PRC despite representing only 15% of the light exposure duration, consistent with previous studies showing a non-linear duration–response function for the effects of light on circadian resetting.

Human phase response curve to a 1 h pulse of bright white light

PubMed Central

St Hilaire, Melissa A; Gooley, Joshua J; Khalsa, Sat Bir S; Kronauer, Richard E; Czeisler, Charles A; Lockley, Steven W

2012-01-01

The phase resetting response of the human circadian pacemaker to light depends on the timing of exposure and is described by a phase response curve (PRC). The current study aimed to construct a PRC for a 1 h exposure to bright white light (∼8000 lux) and to compare this PRC to a <3 lux dim background light PRC. These data were also compared to a previously completed 6.7 h bright white light PRC and a <15 lux dim background light PRC constructed under similar conditions. Participants were randomized for exposure to 1 h of either bright white light (n= 18) or <3 lux dim background light (n= 18) scheduled at 1 of 18 circadian phases. Participants completed constant routine (CR) procedures in dim light (<3 lux) before and after the light exposure to assess circadian phase. Phase shifts were calculated as the difference in timing of dim light melatonin onset (DLMO) during pre- and post-stimulus CRs. Exposure to 1 h of bright white light induced a Type 1 PRC with a fitted peak-to-trough amplitude of 2.20 h. No discernible PRC was observed in the <3 lux dim background light PRC. The fitted peak-to-trough amplitude of the 1 h bright light PRC was ∼40% of that for the 6.7 h PRC despite representing only 15% of the light exposure duration, consistent with previous studies showing a non-linear duration–response function for the effects of light on circadian resetting. PMID:22547633

Pretreatment with Pyridoxamine Mitigates Isolevuglandin-associated Retinal Effects in Mice Exposed to Bright Light*

PubMed Central

Charvet, Casey D.; Saadane, Aicha; Wang, Meiyao; Salomon, Robert G.; Brunengraber, Henri; Turko, Illarion V.; Pikuleva, Irina A.

2013-01-01

The benefits of antioxidant therapy for treating age-related macular degeneration, a devastating retinal disease, are limited. Perhaps species other than reactive oxygen intermediates should be considered as therapeutic targets. These could be lipid peroxidation products, including isolevuglandins (isoLGs), prototypical and extraordinarily reactive γ-ketoaldehydes that avidly bind to proteins, phospholipids, and DNA and modulate the properties of these biomolecules. We found isoLG adducts in aged human retina but not in the retina of mice kept under dim lighting. Hence, to test whether scavenging of isoLGs could complement or supplant antioxidant therapy, we exposed mice to bright light and found that this insult leads to retinal isoLG-adduct formation. We then pretreated mice with pyridoxamine, a B6 vitamer and efficient scavenger of γ-ketoaldehydes, and found that the levels of retinal isoLG adducts are decreased, and morphological changes in photoreceptor mitochondria are not as pronounced as in untreated animals. Our study demonstrates that preventing the damage to biomolecules by lipid peroxidation products, a novel concept in vision research, is a viable strategy to combat oxidative stress in the retina. PMID:23970548

Why the dim light melatonin onset (DLMO) should be measured before treatment of patients with circadian rhythm sleep disorders.

PubMed

Keijzer, Henry; Smits, Marcel G; Duffy, Jeanne F; Curfs, Leopold M G

2014-08-01

Treatment of circadian rhythm sleep disorders (CRSD) may include light therapy, chronotherapy and melatonin. Exogenous melatonin is increasingly being used in patients with insomnia or CRSD. Although pharmacopoeias and the European food safety authority (EFSA) recommend administering melatonin 1-2 h before desired bedtime, several studies have shown that melatonin is not always effective if administered according to that recommendation. Crucial for optimal treatment of CRSD, melatonin and other treatments should be administered at a time related to individual circadian timing (typically assessed using the dim light melatonin onset (DLMO)). If not administered according to the individual patient's circadian timing, melatonin and other treatments may not only be ineffective, they may even result in contrary effects. Endogenous melatonin levels can be measured reliably in saliva collected at the patient's home. A clinically reliably DLMO can be calculated using a fixed threshold. Diary and polysomnographic sleep-onset time do not reliably predict DLMO or circadian timing in patients with CRSD. Knowing the patient's individual circadian timing by assessing DLMO can improve diagnosis and treatment of CRSD with melatonin as well as other therapies such as light or chronotherapy, and optimizing treatment timing will shorten the time required to achieve results. Copyright © 2013 Elsevier Ltd. All rights reserved.

Multiple night-time light-emitting diode lighting strategies impact grassland invertebrate assemblages.

PubMed

Davies, Thomas W; Bennie, Jonathan; Cruse, Dave; Blumgart, Dan; Inger, Richard; Gaston, Kevin J

2017-07-01

White light-emitting diodes (LEDs) are rapidly replacing conventional outdoor lighting technologies around the world. Despite rising concerns over their impact on the environment and human health, the flexibility of LEDs has been advocated as a means of mitigating the ecological impacts of globally widespread outdoor night-time lighting through spectral manipulation, dimming and switching lights off during periods of low demand. We conducted a three-year field experiment in which each of these lighting strategies was simulated in a previously artificial light naïve grassland ecosystem. White LEDs both increased the total abundance and changed the assemblage composition of adult spiders and beetles. Dimming LEDs by 50% or manipulating their spectra to reduce ecologically damaging wavelengths partially reduced the number of commoner species affected from seven to four. A combination of dimming by 50% and switching lights off between midnight and 04:00 am showed the most promise for reducing the ecological costs of LEDs, but the abundances of two otherwise common species were still affected. The environmental consequences of using alternative lighting technologies are increasingly well established. These results suggest that while management strategies using LEDs can be an effective means of reducing the number of taxa affected, averting the ecological impacts of night-time lighting may ultimately require avoiding its use altogether. © 2017 John Wiley & Sons Ltd.

Oxygenation state and twilight vision at 2438 m.

PubMed

Connolly, Desmond M

2011-01-01

Under twilight viewing conditions, hypoxia, equivalent to breathing air at 3048 m (10,000 ft), compromises low contrast acuity, dynamic contrast sensitivity, and chromatic sensitivity. Selected past experiments have been repeated under milder hypoxia, equivalent to altitude exposure below 2438 m (8000 ft), to further define the influence of oxygenation state on mesopic vision. To assess photopic and mesopic visual function, 12 subjects each undertook three experiments using the Contrast Acuity Assessment test, the Frequency Doubling Perimeter, and the Color Assessment and Diagnosis (CAD) test. Experiments were conducted near sea level breathing 15.2% oxygen (balance nitrogen) and 100% oxygen, representing mild hypobaric hypoxia at 2438 m (8000 ft) and the benefit of supplementary oxygen, respectively. Oxygenation state was a statistically significant determinant of visual performance on all three visual parameters at mesopic, but not photopic, luminance. Mesopic sensitivity was greater with supplementary oxygen, but the magnitude of each hypoxic decrement was slight. Hypoxia elevated mesopic contrast acuity thresholds by approximately 4%; decreased mesopic dynamic contrast sensitivity by approximately 2 dB; and extended mean color ellipse axis length by approximately one CAD unit at mesopic luminance (that is, hypoxia decreased chromatic sensitivity). The results indicate that twilight vision may be susceptible to conditions of altered oxygenation at upper-to-mid mesopic luminance with relevance to contemporary night flying, including using night vision devices. Supplementary oxygen should be considered when optimal visual performance is mission-critical during flight above 2438 m (8000 ft) in dim light.

Light-Induced Translocation of RGS9-1 and Gβ5L in Mouse Rod Photoreceptors

PubMed Central

Tian, Mei; Zallocchi, Marisa; Wang, Weimin; Chen, Ching-Kang; Palczewski, Krzysztof; Delimont, Duane; Cosgrove, Dominic; Peng, You-Wei

2013-01-01

The transducin GTPase-accelerating protein complex, which determines the photoresponse duration of photoreceptors, is composed of RGS9-1, Gβ5L and R9AP. Here we report that RGS9-1 and Gβ5L change their distribution in rods during light/dark adaptation. Upon prolonged dark adaptation, RGS9-1 and Gβ5L are primarily located in rod inner segments. But very dim-light exposure quickly translocates them to the outer segments. In contrast, their anchor protein R9AP remains in the outer segment at all times. In the dark, Gβ5L's interaction with R9AP decreases significantly and RGS9-1 is phosphorylated at S475 to a significant degree. Dim light exposure leads to quick de-phosphorylation of RGS9-1. Furthermore, after prolonged dark adaptation, RGS9-1 and transducin Gα are located in different cellular compartments. These results suggest a previously unappreciated mechanism by which prolonged dark adaptation leads to increased light sensitivity in rods by dissociating RGS9-1 from R9AP and redistributing it to rod inner segments. PMID:23555598

On the Detection of Coronal Dimmings and the Extraction of Their Characteristic Properties

NASA Astrophysics Data System (ADS)

Dissauer, K.; Veronig, A. M.; Temmer, M.; Podladchikova, T.; Vanninathan, K.

2018-03-01

Coronal dimmings are distinct phenomena associated with coronal mass ejections (CMEs). The study of coronal dimmings and the extraction of their characteristic parameters help us to obtain additional information regarding CMEs, especially on the initiation and early evolution of Earth-directed CMEs. We present a new approach to detect coronal dimming regions based on a thresholding technique applied on logarithmic base-ratio images. Characteristic dimming parameters describing the dynamics, morphology, magnetic properties, and the brightness of coronal dimming regions are extracted by cumulatively summing newly dimmed pixels over time. It is also demonstrated how core dimming regions are identified as a subset of the overall identified dimming region. We successfully apply our method to two well-observed coronal dimming events. For both events, the core dimming regions are identified and the spatial evolution of the dimming area reveals the expansion of the dimming region around these footpoints. We also show that in the early impulsive phase of the dimming expansion the total unsigned magnetic flux involved in the dimming regions is balanced and that up to 30% of this flux results from the localized core dimming regions. Furthermore, the onset in the profile of the area growth rate is cotemporal with the start of the associated flares and in one case also with the fast rise of the CME, indicating a strong relationship of coronal dimmings with both flares and CMEs.

Adaptive Optics For Imaging Bright Objects Next To Dim Ones

NASA Technical Reports Server (NTRS)

Shao, Michael; Yu, Jeffrey W.; Malbet, Fabien

1996-01-01

Adaptive optics used in imaging optical systems, according to proposal, to enhance high-dynamic-range images (images of bright objects next to dim objects). Designed to alter wavefronts to correct for effects of scattering of light from small bumps on imaging optics. Original intended application of concept in advanced camera installed on Hubble Space Telescope for imaging of such phenomena as large planets near stars other than Sun. Also applicable to other high-quality telescopes and cameras.

Effects of artificial dawn on subjective ratings of sleep inertia and dim light melatonin onset.

PubMed

Giménez, Marina C; Hessels, Martijn; van de Werken, Maan; de Vries, Bonnie; Beersma, Domien G M; Gordijn, Marijke C M

2010-07-01

The timing of work and social requirements has a negative impact on performance and well-being of a significant proportion of the population in our modern society due to a phenomenon known as social jetlag. During workdays, in the early morning, late chronotypes, in particular, suffer from a combination of a nonoptimal circadian phase and sleep deprivation. Sleep inertia, a transient period of lowered arousal after awakening, therefore, becomes more severe. In the present home study, the authors tested whether the use of an alarm clock with artificial dawn could reduce complaints of sleep inertia in people having difficulties in waking up early. The authors also examined whether these improvements were accompanied by a shift in the melatonin rhythm. Two studies were performed: Study 1: three conditions (0, 50, and 250 lux) and Study 2: two conditions (0 lux and self-selected dawn-light intensity). Each condition lasted 2 weeks. In both studies, the use of the artificial dawn resulted in a significant reduction of sleep inertia complaints. However, no significant shift in the onset of melatonin was observed after 2 weeks of using the artificial dawn of 250 lux or 50 lux compared to the control condition. A multilevel analysis revealed that only the presence of the artificial dawn, rather than shift in the dim light melatonin onset or timing of sleep offset, is related to the observed reduction of sleep inertia complaints. Mechanisms other than shift of circadian rhythms are needed to explain the positive results on sleep inertia of waking up with a dawn signal.

ChR2 mutants at L132 and T159 with improved operational light sensitivity for vision restoration.

PubMed

Pan, Zhuo-Hua; Ganjawala, Tushar H; Lu, Qi; Ivanova, Elena; Zhang, Zhifei

2014-01-01

The ectopic expression of microbial opsin-based optogenetic sensors, such as channelrhodopsin-2 (ChR2) in surviving inner retinal neurons, is a promising approach to restoring vision after retinal degeneration. However, a major limitation in using native ChR2 as a light sensor for vision restoration is the low light sensitivity of its expressing cells. Recently, two ChR2 mutations, T159C and L132C, were reported to produce higher photocurrents or have ultra light sensitivity. In this study, we created additional ChR2 mutants at these two sites to search for more light responsive ChR2 forms and evaluate their suitability for vision restoration by examining their light responsive properties in HEK cells and mouse retinal ganglion cells. We found additional ChR2 mutants at these two sites that showed a further increase in current amplitude at low light levels in the cells expressing these mutants, or operational light sensitivity. However, the increase in the operational light sensitivity was correlated with a decrease in temporal kinetics. Therefore, there is a trade-off between operational light sensitivity and temporal resolution for these more light responsive ChR2 mutants. Our results showed that for the two most light responsive mutants, L132C/T159C and L132C/T159S, the required light intensities for generating the threshold spiking activity in retinal ganglion cells were 1.5 and nearly 2 log units lower than wild-type ChR2 (wt-ChR2), respectively. Additionally, their ChR2-mediated spiking activities could follow flicker frequencies up to 20 and 10 Hz, respectively, at light intensities up to 1.5 log units above their threshold levels. Thus, the use of these more light responsive ChR2 mutants could make the optogenetic approach to restoring vision more feasible.

Seeing in the dark: vision and visual behaviour in nocturnal bees and wasps.

PubMed

Warrant, Eric J

2008-06-01

In response to the pressures of predation, parasitism and competition for limited resources, several groups of (mainly) tropical bees and wasps have independently evolved a nocturnal lifestyle. Like their day-active (diurnal) relatives, these insects possess apposition compound eyes, a relatively light-insensitive eye design that is best suited to vision in bright light. Despite this, nocturnal bees and wasps are able to forage at night, with many species capable of flying through a dark and complex forest between the nest and a foraging site, a behaviour that relies heavily on vision and is limited by light intensity. In the two best-studied species - the Central American sweat bee Megalopta genalis (Halictidae) and the Indian carpenter bee Xylocopa tranquebarica (Apidae) - learned visual landmarks are used to guide foraging and homing. Their apposition eyes, however, have only around 30 times greater optical sensitivity than the eyes of their closest diurnal relatives, a fact that is apparently inconsistent with their remarkable nocturnal visual abilities. Moreover, signals generated in the photoreceptors, even though amplified by a high transduction gain, are too noisy and slow to transmit significant amounts of information in dim light. How have nocturnal bees and wasps resolved these paradoxes? Even though this question remains to be answered conclusively, a mounting body of theoretical and experimental evidence suggests that the slow and noisy visual signals generated by the photoreceptors are spatially summed by second-order monopolar cells in the lamina, a process that could dramatically improve visual reliability for the coarser and slower features of the visual world at night.

Visual summation in night-flying sweat bees: a theoretical study.

PubMed

Theobald, Jamie Carroll; Greiner, Birgit; Wcislo, William T; Warrant, Eric J

2006-07-01

Bees are predominantly diurnal; only a few groups fly at night. An evolutionary limitation that bees must overcome to inhabit dim environments is their eye type: bees possess apposition compound eyes, which are poorly suited to vision in dim light. Here, we theoretically examine how nocturnal bees Megalopta genalis fly at light levels usually reserved for insects bearing more sensitive superposition eyes. We find that neural summation should greatly increase M. genalis's visual reliability. Predicted spatial summation closely matches the morphology of laminal neurons believed to mediate such summation. Improved reliability costs acuity, but dark adapted bees already suffer optical blurring, and summation further degrades vision only slightly.

Estimating the dim light melatonin onset of adolescents within a 6-h sampling window: the impact of sampling rate and threshold method.

PubMed

Crowley, Stephanie J; Suh, Christina; Molina, Thomas A; Fogg, Louis F; Sharkey, Katherine M; Carskadon, Mary A

2016-04-01

Circadian rhythm sleep-wake disorders (CRSWDs) often manifest during the adolescent years. Measurement of circadian phase such as the dim light melatonin onset (DLMO) improves diagnosis and treatment of these disorders, but financial and time costs limit the use of DLMO phase assessments in clinic. The current analysis aims to inform a cost-effective and efficient protocol to measure the DLMO in older adolescents by reducing the number of samples and total sampling duration. A total of 66 healthy adolescents (26 males) aged 14.8-17.8 years participated in a study; they were required to sleep on a fixed baseline schedule for a week before which they visited the laboratory for saliva collection in dim light (<20 lux). Two partial 6-h salivary melatonin profiles were derived for each participant. Both profiles began 5 h before bedtime and ended 1 h after bedtime, but one profile was derived from samples taken every 30 min (13 samples) and the other from samples taken every 60 min (seven samples). Three standard thresholds (first three melatonin values mean + 2 SDs, 3 pg/mL, and 4 pg/mL) were used to compute the DLMO. An agreement between DLMOs derived from 30-min and 60-min sampling rates was determined using Bland-Altman analysis; agreement between the sampling rate DLMOs was defined as ± 1 h. Within a 6-h sampling window, 60-min sampling provided DLMO estimates within ± 1 h of DLMO from 30-min sampling, but only when an absolute threshold (3 or 4 pg/mL) was used to compute the DLMO. Future analyses should be extended to include adolescents with CRSWDs. Copyright © 2016 Elsevier B.V. All rights reserved.

Estimating the dim light melatonin onset of adolescents within a 6-h sampling window: the impact of sampling rate and threshold method

PubMed Central

Crowley, Stephanie J.; Suh, Christina; Molina, Thomas A.; Fogg, Louis F.; Sharkey, Katherine M.; Carskadon, Mary A.

2016-01-01

Objective/Background Circadian rhythm sleep-wake disorders often manifest during the adolescent years. Measurement of circadian phase such as the Dim Light Melatonin Onset (DLMO) improves diagnosis and treatment of these disorders, but financial and time costs limit the use of DLMO phase assessments in clinic. The current analysis aims to inform a cost-effective and efficient protocol to measure the DLMO in older adolescents by reducing the number of samples and total sampling duration. Patients/Methods A total of 66 healthy adolescents (26 males) aged 14.8 to 17.8 years participated in a study in which sleep was fixed for one week before they came to the laboratory for saliva collection in dim light (<20 lux). Two partial 6-h salivary melatonin profiles were derived for each participant. Both profiles began 5 h before bedtime and ended 1 h after bedtime, but one profile was derived from samples taken every 30 mins (13 samples) and the other from samples taken every 60 mins (7 samples). Three standard thresholds (first 3 melatonin values mean + 2 SDs, 3 pg/mL, and 4 pg/mL) were used to compute the DLMO. Agreement between DLMOs derived from 30-min and 60-min sampling rates was determined using a Bland-Altman analysis; agreement between sampling rate DLMOs was defined as ± 1 h. Results and Conclusions Within a 6-h sampling window, 60-min sampling provided DLMO estimates that were within ± 1 h of DLMO from 30-min sampling, but only when an absolute threshold (3 pg/mL or 4 pg/mL) was used to compute the DLMO. Future analyses should be extended to include adolescents with circadian rhythm sleep-wake disorders. PMID:27318227

Which lamp will be optimum to eye? Incandescent, fluorescent or LED etc

PubMed Central

Chen, Liang; Zhang, Xiao-Wei

2014-01-01

Low frequency flicker, high frequency flicker, strong light, strong blue light, infrared, ultraviolet, electromagnetic radiation, ripple flicker and dimming flicker produced by different lamps have negative impact on vision, eyes and health. Negative impact on eyes resulting in myopia or cataract etc: the solution is to remove all the negative factors by applying upright lighting technology and that is optimum to vision, eyes and health. PMID:24634884

Which lamp will be optimum to eye? Incandescent, fluorescent or LED etc.

PubMed

Chen, Liang; Zhang, Xiao-Wei

2014-01-01

Low frequency flicker, high frequency flicker, strong light, strong blue light, infrared, ultraviolet, electromagnetic radiation, ripple flicker and dimming flicker produced by different lamps have negative impact on vision, eyes and health. Negative impact on eyes resulting in myopia or cataract etc: the solution is to remove all the negative factors by applying upright lighting technology and that is optimum to vision, eyes and health.

Depressive-like behavior is elevated among offspring of parents exposed to dim light at night prior to mating.

PubMed

Cissé, Yasmine M; Russart, Kathryn L G; Nelson, Randy J

2017-09-01

Rates of major depressive disorder (MDD) have steadily increased over the past 50 years. Many factors have been implicated in the etiology of depressive disorders and environmental influences are being increasingly recognized. The increase in depression rates has coincided with increased artificial nighttime lighting. Exposure to light at night (LAN) has been associated with increased depressive-like behavior in rodents and decreased mood in humans. However, relatively little is known on the multigenerational effects of dLAN on affect. In this study, we exposed adult male and female Siberian hamsters (Phodopus sungorus) to either DARK (0lx) or dim LAN (5lx) for 9 weeks, then paired animals in a full factorial design; all animals were thereafter housed in dark nights. Offspring were gestated and reared in dark nights, then tested in adulthood for depressive-like behaviors and hippocampal expression of glucocorticoid (GR) and melatonin (MT1) receptor expression. Maternal exposure to dLAN decreased sucrose preference, time to first float bout in the Porsolt swim test, and GR expression in the hippocampus. Paternal exposure to dLAN increased time spent floating, and increased hippocampal GR expression. Overall, our results suggest that chronic exposure of parents to light at night has multigenerational effects on offspring depressive-like behavior. If these results pertain to humans, then our data suggest that LAN may contribute to the rapidly rising rates of major depressive disorder in industrialized and developing countries. Copyright © 2017 Elsevier Ltd. All rights reserved.

Dim Light Melatonin Onset and Affect in Adolescents With an Evening Circadian Preference.

PubMed

Dolsen, Michael R; Harvey, Allison G

2018-01-01

A shift toward an evening circadian preference and the onset of mood problems often occur during adolescence. Although these changes are linked to poorer outcomes, few studies have considered how positive and negative affect are related to the circadian rhythm during adolescence. This study examined the relationship between evening and morning affect ratings and dim light melatonin onset (DLMO), a measure of endogenous circadian rhythm. Age and sex were tested as moderators. This study is based on a subset of 163 (94 female, age = 14.7) adolescents with an evening circadian preference from a National Institute of Child Health and Human Development-funded study. Participants provided saliva for melatonin analysis and rated evening and morning affect. Higher evening negative affect was related to a later DLMO. Evening positive affect was not significantly related to DLMO timing. Age but not sex was a significant moderator such that higher negative and lower positive affect were related to a later DLMO for 10- to 13-year-olds, whereas higher positive affect was related to a later DLMO for 17- to 18-year-olds. DLMO was not significantly related to morning affect ratings. There is evidence that higher negative and lower positive affect may be related to the shift toward an evening circadian preference observed in adolescents, particularly for younger adolescents. Copyright © 2017 The Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.

Tracking G-protein-coupled receptor activation using genetically encoded infrared probes.

PubMed

Ye, Shixin; Zaitseva, Ekaterina; Caltabiano, Gianluigi; Schertler, Gebhard F X; Sakmar, Thomas P; Deupi, Xavier; Vogel, Reiner

2010-04-29

Rhodopsin is a prototypical heptahelical family A G-protein-coupled receptor (GPCR) responsible for dim-light vision. Light isomerizes rhodopsin's retinal chromophore and triggers concerted movements of transmembrane helices, including an outward tilting of helix 6 (H6) and a smaller movement of H5, to create a site for G-protein binding and activation. However, the precise temporal sequence and mechanism underlying these helix rearrangements is unclear. We used site-directed non-natural amino acid mutagenesis to engineer rhodopsin with p-azido-l-phenylalanine residues incorporated at selected sites, and monitored the azido vibrational signatures using infrared spectroscopy as rhodopsin proceeded along its activation pathway. Here we report significant changes in electrostatic environments of the azido probes even in the inactive photoproduct Meta I, well before the active receptor state was formed. These early changes suggest a significant rotation of H6 and movement of the cytoplasmic part of H5 away from H3. Subsequently, a large outward tilt of H6 leads to opening of the cytoplasmic surface to form the active receptor photoproduct Meta II. Thus, our results reveal early conformational changes that precede larger rigid-body helix movements, and provide a basis to interpret recent GPCR crystal structures and to understand conformational sub-states observed during the activation of other GPCRs.

Exposure to bright light for several hours during the daytime lowers tympanic temperature

NASA Astrophysics Data System (ADS)

Aizawa, Seika; Tokura, H.

The present study investigates the effect on thympanic temperature of exposure to different light intensities for several hours during the daytime. Nine healthy young adult volunteers (two male, seven female) were exposed to bright light of 4000 lx or dim light of 100 lx during the daytime from 0930 to 1800 hours; the light condition was then kept at 100 lx for a further hour. Tympanic temperature was measured continuously at a neutral condition (28° C, 60% relative humidity) from 1000 to 1800 hours. Urinary samples were collected from 1100 to 1900 hours every 2 h, and melatonin excretion rate was measured by enzyme immunoassay. Of nine subjects, six showed clearly lower tympanic temperatures in the bright compared with the dim condition from 1400 to 1800 hours. Average tympanic temperatures were significantly lower in the bright than in the dim condition from 1645 to 1800 hours. Melatonin excretion rate tended to be higher in the bright than in the dim condition. It was concluded that exposure to bright light of 4000 lx during the daytime for several hours could reduce tympanic temperature, compared with that measured in dim light of 100 lx.

Exposure to bright light for several hours during the daytime lowers tympanic temperature.

PubMed

Aizawa, S; Tokura, H

1997-11-01

The present study investigates the effect on thympanic temperature of exposure to different light intensities for several hours during the daytime. Nine healthy young adult volunteers (two male, seven female) were exposed to bright light of 4000 lx or dim light of 100 lx during the daytime from 0930 to 1800 hours; the light condition was then kept at 100 lx for a further hour. Tympanic temperature was measured continuously at a neutral condition (28 degrees C, 60% relative humidity) from 1000 to 1800 hours. Urinary samples were collected from 1100 to 1900 hours every 2 h, and melatonin excretion rate was measured by enzyme immunoassay. Of nine subjects, six showed clearly lower tympanic temperatures in the bright compared with the dim condition from 1400 to 1800 hours. Average tympanic temperatures were significantly lower in the bright than in the dim condition from 1645 to 1800 hours. Melatonin excretion rate tended to be higher in the bright than in the dim condition. It was concluded that exposure to bright light of 4000 lx during the daytime for several hours could reduce tympanic temperature, compared with that measured in dim light of 100 lx.

SYMPATHETIC FILAMENT ERUPTIONS CONNECTED BY CORONAL DIMMINGS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jiang Yunchun; Yang Jiayan; Hong Junchao

2011-09-10

We present for the first time detailed observations of three successive, interdependent filament eruptions that occurred one by one within 5 hr from different locations beyond the range of a single active region. The first eruption was observed from an active region and was associated with a coronal mass ejection (CME), during which diffuse and complex coronal dimmings formed, largely extending to the two other filaments located in quiet-Sun regions. Then, both quiescent filaments consecutively underwent the second and third eruptions, while the nearby dimmings were persistent. Comparing the result of a derived coronal magnetic configuration, the magnetic connectivity betweenmore » the dimmings suggested that they were caused by the joint effect of simple expansion of overlying loop systems forced by the first eruption, as well as by its erupting field interacting or reconnecting with the surrounding magnetic structures. Note that the dimming process in the first eruption indicated a weakening and partial removal of an overlying magnetic field constraint on the two other filaments, and thus one can physically connect these eruptions as sympathetic. It appears that the peculiar magnetic field configuration in our event was largely favorable to the occurrence of sympathetic filament eruptions. Because coronal dimmings are frequent and common phenomena in solar eruptions, especially in CME events, it is very likely that they represent a universal agent that can link consecutive eruptions nearby with sympathetic eruptions.« less

Evolution of Vision

NASA Astrophysics Data System (ADS)

Ostrovsky, Mikhail

The evolution of photoreception, giving rise to eye, offers a kaleidoscopic view on selection acting at both the organ and molecular levels. The molecular level is mainly considered in the lecture. The greatest progress to date has been made in relation to the opsin visual pigments. Opsins appeared before eyes did. Two- and three-dimensional organization for rhodopsin in the rod outer segment disk membrane, as well as molecular mechanisms of visual pigments spectral tuning, photoisomerization and also opsin as a G-protein coupled receptor are considered. Molecular mechanisms of visual pigments spectral tuning, namely switching of chromophore (physiological time scale) and amino acid changes in the chromophore site of opsin (evolutionary time scale) is considered in the lecture. Photoisomerization of rhodopsin chromophore, 11-cis retinal is the only photochemical reaction in vision. The reaction is extemely fast (less that 200 fs) and high efficient (. is 0.65). The rhodopsin photolysis and kinetics of the earlier products appearance, photo- and bathorhodopsin, is considered. It is known that light is not only a carrier of information, but also a risk factor of damage to the eye. This photobiological paradox of vision is mainly due to the nature of rhodopsin chromophore. Photooxidation is the base of the paradox. All factors present in the phototrceptor cells to initiate free-radical photooxidation: photosensitizers, oxygen and substrates of oxidation: lipids and proteins (opsin). That is why photoprotective system of the eye structures appeared in the course of evolution. Three lines of protective system to prevent light damage to the retina and retina pigment epithelium is known: permanent renewal of rod and cone outer segment, powerful antioxidant system and optical media as cut-off filters where the lens is a key component. The molecular mechanisms of light damage to the eye and photoprotective system of the eye is considered in the lecture. The molecular

Exposure to bright light biases effort-based decisions.

PubMed

Bijleveld, Erik; Knufinke, Melanie

2018-06-01

Secreted in the evening and the night, melatonin suppresses activity of the mesolimbic dopamine pathway, a brain pathway involved in reward processing. However, exposure to bright light diminishes-or even prevents-melatonin secretion. Thus, we hypothesized that reward processing, in the evening, is more pronounced in bright light (vs. dim light). Healthy human participants carried out three tasks that tapped into various aspects of reward processing (effort expenditure for rewards task [EEfRT]; two-armed bandit task [2ABT]; balloon analogue risk task [BART). Brightness was manipulated within-subjects (bright vs. dim light), in separate evening sessions. During the EEfRT, participants used reward-value information more strongly when they were exposed to bright light (vs. dim light). This finding supported our hypothesis. However, exposure to bright light did not significantly affect task behavior on the 2ABT and the BART. While future research is necessary (e.g., to zoom in on working mechanisms), these findings have potential implications for the design of physical work environments. (PsycINFO Database Record (c) 2018 APA, all rights reserved).

Infrared dim target detection based on visual attention

NASA Astrophysics Data System (ADS)

Wang, Xin; Lv, Guofang; Xu, Lizhong

2012-11-01

Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. Based on human visual attention mechanisms, an automatic detection algorithm for infrared dim target is presented. After analyzing the characteristics of infrared dim target images, the method firstly designs Difference of Gaussians (DoG) filters to compute the saliency map. Then the salient regions where the potential targets exist in are extracted by searching through the saliency map with a control mechanism of winner-take-all (WTA) competition and inhibition-of-return (IOR). At last, these regions are identified by the characteristics of the dim IR targets, so the true targets are detected, and the spurious objects are rejected. The experiments are performed for some real-life IR images, and the results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

Isolation of a circulating CD45−, CD34dim cell population and validation of their endothelial phenotype

PubMed Central

Tropea, Margaret M.; Harper, Bonnie J. A.; Graninger, Grace M.; Phillips, Terry M.; Ferreyra, Gabriela; Mostowski, Howard S.; Danner, Robert L.; Suffredini, Anthony F.; Solomon, Michael A.

2016-01-01

Summary Accurately detecting circulating endothelial cells (CECs) is important since their enumeration has been proposed as a biomarker to measure injury to the vascular endothelium. However, there is no single methodology for determining CECs in blood, making comparison across studies difficult. Many methods for detecting CECs rely on characteristic cell surface markers and cell viability indicators, but lack secondary validation. Here, a CEC population in healthy adult human subjects was identified by flow cytometry as CD45−, CD34dim that is comparable to a previously described CD45−, CD31bright population. In addition, nuclear staining with 7-aminoactinomycin D (7-AAD) was employed as a standard technique to exclude dead cells. Unexpectedly, the CD45−, CD34dim, 7-AAD− CECs lacked surface detectable CD146, a commonly used marker of CECs. Furthermore, light microscopy revealed this cell population to be composed primarily of large cells without a clearly defined nucleus. Nevertheless, immunostains still demonstrated the presence of the lectin Ulex europaeus and van Willebrand factor. Ultramicro analytical immunochemistry assays for the endothelial cell proteins CD31, CD34, CD62E, CD105, CD141, CD144 and vWF indicated these cells possess an endothelial phenotype. However, only a small amount of RNA, which was mostly degraded, could be isolated from these cells. Thus the majority of CECs in healthy individuals as defined by CD45−, CD34dim, and 7-AAD− have shed their CD146 surface marker and are senescent cells without an identifiable nucleus and lacking RNA of sufficient quantity and quality for transcriptomal analysis. This study highlights the importance of secondary validation of CEC identification. PMID:25057108

Effects of tryptophan-rich breakfast and light exposure during the daytime on melatonin secretion at night.

PubMed

Fukushige, Haruna; Fukuda, Yumi; Tanaka, Mizuho; Inami, Kaoru; Wada, Kai; Tsumura, Yuki; Kondo, Masayuki; Harada, Tetsuo; Wakamura, Tomoko; Morita, Takeshi

2014-11-19

The purpose of the present study is to investigate effects of tryptophan intake and light exposure on melatonin secretion and sleep by modifying tryptophan ingestion at breakfast and light exposure during the daytime, and measuring sleep quality (by using actigraphy and the OSA sleep inventory) and melatonin secretion at night. Thirty three male University students (mean ± SD age: 22 ± 3.1 years) completed the experiments lasting 5 days and 4 nights. The subjects were randomly divided into four groups: Poor*Dim (n = 10), meaning a tryptophan-poor breakfast (55 mg/meal) in the morning and dim light environment (<50 lx) during the daytime; Rich*Dim (n = 7), tryptophan-rich breakfast (476 mg/meal) and dim light environment; Poor*Bright (n = 9), tryptophan-poor breakfast and bright light environment (>5,000 lx); and Rich*Bright (n = 7), tryptophan-rich breakfast and bright light. Saliva melatonin concentrations on the fourth day were significantly lower than on the first day in the Poor*Dim group, whereas they were higher on the fourth day in the Rich*Bright group. Creatinine-adjusted melatonin in urine showed the same direction as saliva melatonin concentrations. These results indicate that the combination of a tryptophan-rich breakfast and bright light exposure during the daytime could promote melatonin secretion at night; further, the observations that the Rich*Bright group had higher melatonin concentrations than the Rich*Dim group, despite no significant differences being observed between the Poor*Dim and Rich*Dim groups nor the Poor*Bright and Rich*Bright groups, suggest that bright light exposure in the daytime is an important contributor to raised melatonin levels in the evening. This study is the first to report the quantitative effects of changed tryptophan intake at breakfast combined with daytime light exposure on melatonin secretion and sleep quality. Evening saliva melatonin secretion changed significantly and indicated that a tryptophan

Dim target detection method based on salient graph fusion

NASA Astrophysics Data System (ADS)

Hu, Ruo-lan; Shen, Yi-yan; Jiang, Jun

2018-02-01

Dim target detection is one key problem in digital image processing field. With development of multi-spectrum imaging sensor, it becomes a trend to improve the performance of dim target detection by fusing the information from different spectral images. In this paper, one dim target detection method based on salient graph fusion was proposed. In the method, Gabor filter with multi-direction and contrast filter with multi-scale were combined to construct salient graph from digital image. And then, the maximum salience fusion strategy was designed to fuse the salient graph from different spectral images. Top-hat filter was used to detect dim target from the fusion salient graph. Experimental results show that proposal method improved the probability of target detection and reduced the probability of false alarm on clutter background images.

Traffic light detection and intersection crossing using mobile computer vision

NASA Astrophysics Data System (ADS)

Grewei, Lynne; Lagali, Christopher

2017-05-01

The solution for Intersection Detection and Crossing to support the development of blindBike an assisted biking system for the visually impaired is discussed. Traffic light detection and intersection crossing are key needs in the task of biking. These problems are tackled through the use of mobile computer vision, in the form of a mobile application on an Android phone. This research builds on previous Traffic Light detection algorithms with a focus on efficiency and compatibility on a resource-limited platform. Light detection is achieved through blob detection algorithms utilizing training data to detect patterns of Red, Green and Yellow in complex real world scenarios where multiple lights may be present. Also, issues of obscurity and scale are addressed. Safe Intersection crossing in blindBike is also discussed. This module takes a conservative "assistive" technology approach. To achieve this blindBike use's not only the Android device but, an external bike cadence Bluetooth/Ant enabled sensor. Real world testing results are given and future work is discussed.

Tracheal intubation by novice staff: the direct vision laryngoscope or the lighted stylet (Trachlight)?

PubMed Central

Soh, C; Kong, C; Kong, C; Ip-Yam, P; Chin, E; Goh, M

2002-01-01

Objective: To compare the ease of use of the direct vision laryngoscope and the lighted stylet (Trachlight) by novice staff. Methods: Ten novice medical officers (MOs) performed orotracheal intubations using either the conventional direct vision laryngoscope (DL) or a lighted stylet device (Trachlight). They performed their DL intubations during the first phase of the study, followed by the Trachlight intubations in the subsequent phase. Results: 51 of 54 (94%) of the DL intubation attempts were successful compared with 36 of 54 (67%) of the Trachlight intubations (p<0.001). The mean (SEM) time for intubation was 44 (7) seconds in the DL group and 66 (13) seconds in the Trachlight group (p=0.004). In addition 45 of 54 (83%) of the DL intubations were successful at the first attempt versus 15 of 54 (28%) in the Trachlight group (p<0.001). Conclusion: The results show that the use of the conventional direct vision laryngoscope in novices is associated with significantly shorter mean intubation times and higher success rates on the first attempt compared with the Trachlight. PMID:12101133

Clinical efficacy of dim light melatonin onset testing in diagnosing delayed sleep phase syndrome.

PubMed

Rahman, Shadab A; Kayumov, Leonid; Tchmoutina, Ekaterina A; Shapiro, Colin M

2009-05-01

Delayed Sleep Phase Syndrome (DSPS) arises from biological clock desynchrony and accounts for 10% of chronic insomnia patients. Currently DSPS is diagnosed based on sleep/wake cycle disruptions rather than examining the underlying biological clock alterations. The objective of the study was to determine the sensitivity and specificity of the Dim Light Melatonin Onset (DLMO) Test in diagnosing DSPS in a clinical setting. Fifty-six patients (mean age 28 years) symptomatic of DSPS participated in the study. Following an initial assessment of DSPS using sleep diaries, participants underwent two consecutive nights of polysomnography (PSG), with an imposed sleep period on the second night to demonstrate the delay in the timing of habitual sleep period and to thereby confirm DSPS. Circadian phase delays were also measured using melatonin secretion profiles, and the efficacy of diagnosing DSPS using DLMO was compared to using sleep diaries and PSG. Melatonin secretion was assayed for each individual by ELISA using saliva samples. Main outcome measures included the time of melatonin secretion onset, clinical sensitivity and specificity of the DLMO test. The time of melatonin secretion onset was significantly delayed in DSPS patients. Clinical sensitivity and specificity of the DLMO test in diagnosing DSPS were 90.3% and 84.0%, respectively. The DLMO test is an accurate tool for differentiating between sleep disorder patients with or without underlying circadian rhythm disruption. It is effective for phase typing DSPS patients in a clinical setting.

Impact of Exposure to Dim Light at Night on Sleep in Female and Comparison with Male Subjects.

PubMed

Cho, Chul-Hyun; Yoon, Ho-Kyoung; Kang, Seung-Gul; Kim, Leen; Lee, Eun-Il; Lee, Heon-Jeong

2018-03-19

Light pollution has become a social and health issue. We performed an experimental study to investigate impact of dim light at night (dLAN) on sleep in female subjects, with measurement of salivary melatonin. The 25 female subjects (Group A: 12; Group B: 13 subjects) underwent a nocturnal polysomnography (NPSG) session with no light (Night 1) followed by an NPSG session randomly assigned to two conditions (Group A: 5; Group B: 10 lux) during a whole night of sleep (Night 2). Salivary melatonin was measured before and after sleep on each night. For further investigation, the female and male subjects of our previous study were collected (48 subjects), and differences according to gender were compared. dLAN during sleep was significantly associated with decreased total sleep time (TST; F=4.818, p=0.039), sleep efficiency (SE; F=5.072, p=0.034), and Stage R latency (F=4.664, p=0.041) for female subjects, and decreased TST (F=14.971, p<0.001) and SE (F=7.687, p=0.008), and increased wake time after sleep onset (F=6.322, p=0.015) and Stage R (F=5.031, p=0.03), with a night-group interaction (F=4.579, p=0.038) for total sample. However, no significant melatonin changes. There was no significant gender difference of the impact of dLAN on sleep, showing the negative changes in the amount and quality of sleep and the increase in REM sleep in the both gender group under 10 lux condition. We found a negative impact of exposure to dLAN on sleep in female as well as in merged subjects. REM sleep showed a pronounced increase under 10 lux than under 5 lux in merged subjects, suggesting the possibility of subtle influences of dLAN on REM sleep.

Light, Imaging, Vision: An interdisciplinary undergraduate course

NASA Astrophysics Data System (ADS)

Nelson, Philip

2015-03-01

The vertebrate eye is fantastically sensitive instrument, capable of registering the absorption of a single photon, and yet generating very low noise. Using eyes as a common thread helps motivate undergraduates to learn a lot of physics, both fundamental and applied to scientific imaging and neuroscience. I'll describe an undergraduate course, for students in several science and engineering majors, that takes students from the rudiments of probability theory to the quantum character of light, including modern experimental methods like fluorescence imaging and Förster resonance energy transfer. After a digression into color vision, we then see how the Feynman principle explains the apparently wavelike phenomena associated to light, including applications like diffraction, subdiffraction imaging, total internal reflection and TIRF microscopy. Then we see how scientists documented the single-quantum sensitivity of the eye seven decades earlier than ``ought'' to have been possible, and finally close with the remarkable signaling cascade that delivers such outstanding performance. Parts of this story are now embodied in a new textbook (WH Freeman and Co, 1/2015); additional course materials are available upon request. Work supported by NSF Grants EF-0928048 and DMR-0832802.

Dim Networks: The Utility of Social Network Analysis for Illuminating Partner Security Force Networks

DTIC Science & Technology

2015-12-01

use of social network analysis (SNA) has allowed the military to map dark networks of terrorist organizations and selectively target key elements...data to improve SC. 14. SUBJECT TERMS social network analysis, dark networks, light networks, dim networks, security cooperation, Southeast Asia...task may already exist. Recently, the use of social network analysis (SNA) has allowed the military to map dark networks of terrorist organizations

Circadian rhythm adaptation to simulated night shift work: effect of nocturnal bright-light duration.

PubMed

Eastman, C I; Liu, L; Fogg, L F

1995-07-01

We compared bright-light durations of 6, 3 and 0 hours (i.e. dim light) during simulated night shifts for phase shifting the circadian rectal temperature rhythm to align with a 12-hour shift of the sleep schedule. After 10 baseline days there were 8 consecutive night-work, day-sleep days, with 8-hour sleep (dark) periods. The bright light (about 5,000 lux, around the baseline temperature minimum) was used during all 8 night shifts, and dim light was < 500 lux. This was a field study in which subjects (n = 46) went outside after the night shifts and slept at home. Substantial circadian adaptation (i.e. a large cumulative temperature rhythm phase shift) was produced in many subjects in the bright light groups, but not in the dim light group. Six and 3 hours of bright light were each significantly better than dim light for phase shifting the temperature rhythm, but there was no significant difference between 6 and 3 hours. Thus, durations > 3 hours are probably not necessary in similar shift-work situations. Larger temperature rhythm phase shifts were associated with better subjective daytime sleep, less subjective fatigue and better overall mood.

Two- and 4-hour bright-light exposures differentially effect sleepiness and performance the subsequent night.

PubMed

Thessing, V C; Anch, A M; Muehlbach, M J; Schweitzer, P K; Walsh, J K

1994-03-01

The effect of two durations of bright light upon sleepiness and performance during typical night shift hours was assessed. Thirty normal, healthy young adults participated in a 2-night protocol. On the 1st night subjects were exposed to bright or dim light beginning at 2400 hours, under one of the following three conditions: bright light for 4 hours, dim light for 2 hours followed by bright light for 2 hours or dim light for 4 hours. Following light exposure, subjects remained awake until 0800 hours in a dimly lit room and slept in the laboratory between 0800 and 1600 hours, during which time sleep was estimated with actigraphy. Throughout the 2nd night, the multiple sleep latency test (MSLT), simulated assembly line task (SALT) performance, and subjective sleepiness were recorded. The single, 4-hour exposure to bright light was found to significantly increase MSLT scores and improve SALT performance during the early morning hours on the night following bright-light exposure. No significant effects were noted with a 2-hour exposure. The most likely explanation for these findings is a phase delay in the circadian rhythm of sleepiness-alertness.

Coronal Mass Ejections and Dimmings: A Comparative Study using MHD Simulations and SDO Observations

NASA Astrophysics Data System (ADS)

Jin, M.; Cheung, C. M. M.; DeRosa, M. L.; Nitta, N.; Schrijver, K.

2017-12-01

Solar coronal dimmings have been observed extensively in the past two decades. Due to their close association with coronal mass ejections (CMEs), there is a critical need to improve our understanding of the physical processes that cause dimmings and determine their relationship with CMEs. In this study, we investigate coronal dimmings by combining simulation and observational efforts. By utilizing a data-driven global magnetohydrodynamics model (AWSoM: Alfven-wave Solar Model), we simulate coronal dimmings resulting from different CME energetics and flux rope configurations. We synthesize the emissions of different EUV spectral bands/lines and compare with SDO/AIA and EVE observations. A detailed analysis of simulation and observation data suggests that although the transient dimming / brightening patterns could relate to plasma heating processes (either by adiabatic compression or reconnection), the long-lasting "core" and "remote" (also known as "secondary") dimmings both originate from regions with open/quasi-open fields and are caused by mass loss process. The mass loss in the remote dimming region is induced by CME-driven shock. Using metrics such as dimming depth, dimming slope, and recovery time, we investigate the relationship between dimmings and CME properties (e.g., CME mass, CME speed) in the simulation. Our result suggests that coronal dimmings encode important information about CME energetics, CME-driven shock properties, and magnetic configuration of erupting flux ropes. We also discuss how our knowledge about solar coronal dimmings could be extended to the study of stellar CMEs, which may prove important for exoplanet atmospheres and habitability but which are currently not observable.

Usability of light-emitting diodes in precision approach path indicator systems by individuals with marginal color vision.

DOT National Transportation Integrated Search

2014-05-01

To save energy, the FAA is planning to convert from incandescent lights to light-emitting diodes (LEDs) in : precision approach path indicator (PAPI) systems. Preliminary work on the usability of LEDs by color vision-waivered pilots (Bullough, Skinne...

Structurally Simple and Easily Accessible Perylenes for Dye-Sensitized Solar Cells Applicable to Both 1 Sun and Dim-Light Environments.

PubMed

Chou, Hsien-Hsin; Liu, Yu-Chieh; Fang, Guanjie; Cao, Qiao-Kai; Wei, Tzu-Chien; Yeh, Chen-Yu

2017-11-01

The need for low-cost and highly efficient dyes for dye-sensitized solar cells under both the sunlight and dim light environments is growing. We have devised GJ-series push-pull organic dyes which require only four synthesis steps. These dyes feature a linear molecular structure of donor-perylene-ethynylene-arylcarboxylic acid, where donor represents N,N-diarylamino group and arylcarboxylic groups represent benzoic, thienocarboxylic, 2-cyano-3-phenylacrylic, 2-cyano-3-thienoacrylic, and 4-benzo[c][1,2,5]thiadiazol-4-yl-benzoic groups. In this study, we demonstrated that a dye without tedious and time-consuming synthesis efforts can perform efficiently. Under the illumination of AM1.5G simulated sunlight, the benzothiadiazole-benzoic-containing GJ-BP dye shows the best power conversion efficiency (PCE) of 6.16% with V OC of 0.70 V and J SC of 11.88 mA cm -2 using liquid iodide-based electrolyte. It also shows high performance in converting light of 6000 lx light intensity, that is, incident power of ca. 1.75 mW cm -2 , to power output of 0.28 mW cm -2 which equals a PCE of 15.79%. Interestingly, the benzoic-containing dye GJ-P with a simple molecular structure has comparable performance in generating power output of 0.26 mW cm -2 (PCE of 15.01%) under the same condition and is potentially viable toward future application.

Plasma melatonin and insulin-like growth factor-1 responses to dim light at night in dairy heifers.

PubMed

Muthuramalingam, P; Kennedy, A D; Berry, R J

2006-04-01

The effect of dim (5, 10 and 50 1x) light at night on night plasma melatonin level (NML) and night plasma insulin-like growth factor-1 (IGF-1) level was determined in 12 prepubertal Holstein heifers (245 +/- 16 days age) using a 4 x 4 Latin Square design with 14-day treatment and 14-day recovery periods. Blood samples were collected at 23:00 hr (prior to the 8 hr night treatment which commenced at mid-night) on days 0, 3 and 13, and throughout the night at 01:00, 02:00, 03:00, 04:00, 06:00 and 08:00 hr on days 1, 4 and 14 of treatment. Plasma was analysed by radioimmunoassay for melatonin (all samples) and IGF-1 (samples for day 14, 04:00 hr only). Treatment (P = 0.03) and treatment x time (P = 0.02) were significant for NML. Exposure to 50 lx suppressed NML by 50% during the initial 2 hr of the night, but not thereafter. Exposure to 5 and 10 lx had no effect on NML. The NML response to 50 lx was found on all treatment days studied (treatment x time x day; P = 0.99). There was no treatment effect on plasma IGF-1 level (P = 0.89), but plasma IGF-1 level was higher (P = 0.001) during period 4. Plasma IGF-1 level and NML tended (P = 0.10) to be negatively correlated. Light intensities of 10 lx or less appear safe for use at night in dairy barns where darkness is recommended.

Flight performance in night-flying sweat bees suffers at low light levels.

PubMed

Theobald, Jamie Carroll; Coates, Melissa M; Wcislo, William T; Warrant, Eric J

2007-11-01

The sweat bee Megalopta (Hymenoptera: Halictidae), unlike most bees, flies in extremely dim light. And although nocturnal insects are often equipped with superposition eyes, which greatly enhance light capture, Megalopta performs visually guided flight with apposition eyes. We examined how light limits Megalopta's flight behavior by measuring flight times and corresponding light levels and comparing them with flight trajectories upon return to the nest. We found the average time to land increased in dim light, an effect due not to slow approaches, but to circuitous approaches. Some landings, however, were quite fast even in the dark. To explain this, we examined the flight trajectories and found that in dim light, landings became increasingly error prone and erratic, consistent with repeated landing attempts. These data agree well with the premise that Megalopta uses visual summation, sacrificing acuity in order to see and fly at the very dimmest light intensities that its visual system allows.

Morning and Evening Blue-Enriched Light Exposure Alters Metabolic Function in Normal Weight Adults.

PubMed

Cheung, Ivy N; Zee, Phyllis C; Shalman, Dov; Malkani, Roneil G; Kang, Joseph; Reid, Kathryn J

2016-01-01

Increasing evidence points to associations between light-dark exposure patterns, feeding behavior, and metabolism. This study aimed to determine the acute effects of 3 hours of morning versus evening blue-enriched light exposure compared to dim light on hunger, metabolic function, and physiological arousal. Nineteen healthy adults completed this 4-day inpatient protocol under dim light conditions (<20lux). Participants were randomized to 3 hours of blue-enriched light exposure on Day 3 starting either 0.5 hours after wake (n = 9; morning group) or 10.5 hours after wake (n = 10; evening group). All participants remained in dim light on Day 2 to serve as their baseline. Subjective hunger and sleepiness scales were collected hourly. Blood was sampled at 30-minute intervals for 4 hours in association with the light exposure period for glucose, insulin, cortisol, leptin, and ghrelin. Homeostatic model assessment of insulin resistance (HOMA-IR) and area under the curve (AUC) for insulin, glucose, HOMA-IR and cortisol were calculated. Comparisons relative to baseline were done using t-tests and repeated measures ANOVAs. In both the morning and evening groups, insulin total area, HOMA-IR, and HOMA-IR AUC were increased and subjective sleepiness was reduced with blue-enriched light compared to dim light. The evening group, but not the morning group, had significantly higher glucose peak value during blue-enriched light exposure compared to dim light. There were no other significant differences between the morning or the evening groups in response to blue-enriched light exposure. Blue-enriched light exposure acutely alters glucose metabolism and sleepiness, however the mechanisms behind this relationship and its impacts on hunger and appetite regulation remain unclear. These results provide further support for a role of environmental light exposure in the regulation of metabolism.

Morning and Evening Blue-Enriched Light Exposure Alters Metabolic Function in Normal Weight Adults

PubMed Central

Cheung, Ivy N.; Zee, Phyllis C.; Shalman, Dov; Malkani, Roneil G.; Kang, Joseph; Reid, Kathryn J.

2016-01-01

Increasing evidence points to associations between light-dark exposure patterns, feeding behavior, and metabolism. This study aimed to determine the acute effects of 3 hours of morning versus evening blue-enriched light exposure compared to dim light on hunger, metabolic function, and physiological arousal. Nineteen healthy adults completed this 4-day inpatient protocol under dim light conditions (<20lux). Participants were randomized to 3 hours of blue-enriched light exposure on Day 3 starting either 0.5 hours after wake (n = 9; morning group) or 10.5 hours after wake (n = 10; evening group). All participants remained in dim light on Day 2 to serve as their baseline. Subjective hunger and sleepiness scales were collected hourly. Blood was sampled at 30-minute intervals for 4 hours in association with the light exposure period for glucose, insulin, cortisol, leptin, and ghrelin. Homeostatic model assessment of insulin resistance (HOMA-IR) and area under the curve (AUC) for insulin, glucose, HOMA-IR and cortisol were calculated. Comparisons relative to baseline were done using t-tests and repeated measures ANOVAs. In both the morning and evening groups, insulin total area, HOMA-IR, and HOMA-IR AUC were increased and subjective sleepiness was reduced with blue-enriched light compared to dim light. The evening group, but not the morning group, had significantly higher glucose peak value during blue-enriched light exposure compared to dim light. There were no other significant differences between the morning or the evening groups in response to blue-enriched light exposure. Blue-enriched light exposure acutely alters glucose metabolism and sleepiness, however the mechanisms behind this relationship and its impacts on hunger and appetite regulation remain unclear. These results provide further support for a role of environmental light exposure in the regulation of metabolism. PMID:27191727

Protein aggregation studied by forward light scattering and light transmission analysis

NASA Astrophysics Data System (ADS)

Penzkofer, A.; Shirdel, J.; Zirak, P.; Breitkreuz, H.; Wolf, E.

2007-12-01

The aggregation of the circadian blue-light photo-receptor cryptochrome from Drosophila melanogaster (dCry) is studied by transmission and forward light scattering measurement in the protein transparent wavelength region. The light scattering in forward direction is caused by Rayleigh scattering which is proportional to the degree of aggregation. The light transmission through the samples in the transparent region is reduced by Mie light scattering in all directions. It depends on the degree of aggregation and the monomer volume fill factor of the aggregates (less total scattering with decreasing monomer volume fill factor of protein globule) allowing a distinction between tightly packed protein aggregation (monomer volume fill factor 1) and loosely packed protein aggregation (monomer volume fill factor less than 1). An increase in aggregation with temperature, concentration, and blue-light exposure is observed. At a temperature of 4 °C and a protein concentration of less than 0.135 mM no dCry aggregation was observed, while at 24 °C and 0.327 mM gelation occurred (loosely packed aggregates occupying the whole solution volume).

Understanding Coronal Dimming and its Relation to Coronal Mass Ejections

NASA Astrophysics Data System (ADS)

Mason, J. P.; Woods, T. N.; Caspi, A.; Hock, R. A.

2013-12-01

When extreme ultraviolet (EUV) emitting material in the corona is lost during a coronal mass ejection (CME), the solar spectral irradiance is impacted and these effects are observed in data from the Solar Dynamics Observatory (SDO) EUV Variability Experiment (EVE) and Atmospheric Imaging Assembly (AIA). This process is one of the physical mechanisms that can lead to the observation of 'coronal dimming,' a phenomenon lasting eight hours on average and rarely persisting longer than one day. Other mechanisms that can cause observed dimming include obscuration of bright material (e.g., flare arcade) by dark material (e.g., filament), temperature evolution (e.g., cool plasma being heated causing transient decreases in characteristic emission lines), and propagation of global waves. Each of these processes has a unique spectral signature, which will be explained and exemplified. In particular, the 7 August 2010 M1.0 flare with associated ~870 km/s CME will be analyzed in detail using both AIA and EVE to demonstrate new techniques for isolating dimming due to the CME ('core dimming'). Further analysis will estimate CME mass and velocity using only parameterization of core dimming and compare these estimates to traditionally calculated CME kinetics.

The Acute Effects of Intermittent Light Exposure in the Evening on Alertness and Subsequent Sleep Architecture.

PubMed

Yang, Minqi; Ma, Ning; Zhu, Yingying; Su, Ying-Chu; Chen, Qingwei; Hsiao, Fan-Chi; Ji, Yanran; Yang, Chien-Ming; Zhou, Guofu

2018-03-15

Exposure to bright light is typically intermittent in our daily life. However, the acute effects of intermittent light on alertness and sleep have seldom been explored. To investigate this issue, we employed within-subject design and compared the effects of three light conditions: intermittent bright light (30-min pulse of blue-enriched bright light (~1000 lux, ~6000 K) alternating with 30-min dim normal light (~5 lux, ~3600 K) three times); continuous bright light; and continuous dim light on subjective and objective alertness and subsequent sleep structure. Each light exposure was conducted during the three hours before bedtime. Fifteen healthy volunteers (20 ± 3.4 years; seven males) were scheduled to stay in the sleep laboratory for four separated nights (one for adaptation and the others for the light exposures) with a period of at least one week between nights. The results showed that when compared with dim light, both intermittent light and continuous bright light significantly increased subjective alertness and decreased sleep efficiency (SE) and total sleep time (TST). Intermittent light significantly increased objective alertness than dim light did during the second half of the light-exposure period. Our results suggested that intermittent light was as effective as continuous bright light in their acute effects in enhancing subjective and objective alertness and in negatively impacting subsequent sleep.

Light transmission through intraocular lenses with or without yellow chromophore (blue light filter) and its potential influence on functional vision in everyday environmental conditions.

PubMed

Owczarek, Grzegorz; Gralewicz, Grzegorz; Skuza, Natalia; Jurowski, Piotr

2016-01-01

In this research the factors used to evaluate the light transmission through two types of acrylic hydrophobic intraocular lenses, one that contained yellow chromophore that blocks blue light transmission and the other which did not contain that filter, were defined according to various light condition, e.g., daylight and at night. The potential influence of light transmission trough intraocular lenses with or without yellow chromophore on functional vision in everyday environmental conditions was analysed.

Analysis of light emitting diode array lighting system based on human vision: normal and abnormal uniformity condition.

PubMed

Qin, Zong; Ji, Chuangang; Wang, Kai; Liu, Sheng

2012-10-08

In this paper, condition for uniform lighting generated by light emitting diode (LED) array was systematically studied. To take human vision effect into consideration, contrast sensitivity function (CSF) was novelly adopted as critical criterion for uniform lighting instead of conventionally used Sparrow's Criterion (SC). Through CSF method, design parameters including system thickness, LED pitch, LED's spatial radiation distribution and viewing condition can be analytically combined. In a specific LED array lighting system (LALS) with foursquare LED arrangement, different types of LEDs (Lambertian and Batwing type) and given viewing condition, optimum system thicknesses and LED pitches were calculated and compared with those got through SC method. Results show that CSF method can achieve more appropriate optimum parameters than SC method. Additionally, an abnormal phenomenon that uniformity varies with structural parameters non-monotonically in LALS with non-Lambertian LEDs was found and analyzed. Based on the analysis, a design method of LALS that can bring about better practicability, lower cost and more attractive appearance was summarized.

Reading performance of monofocal pseudophakic patients with and without glasses under normal and dim light conditions.

PubMed

Radner, Wolfgang; Radner, Stephan; Raunig, Valerian; Diendorfer, Gabriela

2014-03-01

To evaluate reading performance of patients with monofocal intraocular lenses (IOLs) (Acrysof SN60WF) with or without reading glasses under bright and dim light conditions. Austrian Academy of Ophthalmology, Vienna, Austria. Evaluation of a diagnostic test or technology. In pseudophakic patients, the spherical refractive error was limited to between +0.50 diopter (D) and -0.75 D with astigmatism of 0.75 D (mean spherical equivalent: right eye, -0.08 ± 0.43 [SD]; left eye, -0.15 ± 0.35). Near addition was +2.75 D. Reading performance was assessed binocularly with or without reading glasses at an illumination of 100 candelas (cd)/m(2) and 4 cd/m(2) using the Radner Reading Charts. In the 25 patients evaluated, binocularly, the mean corrected distance visual acuity was -0.07 ± 0.06 logMAR and the mean uncorrected distance visual acuity was 0.01 ± 0.11 logMAR. The mean reading acuity with reading glasses was 0.02 ± 0.10 logRAD at 100 cd/m(2) and 0.12 ± 0.14 logRAD at 4 cd/m(2). Without reading glasses, it was 0.44 ± 0.13 logRAD and 0.56 ± 0.16 logRAD, respectively (P < .05). Without reading glasses and at 100 cd/m(2), 40% of patients read 0.4 logRAD at more than 80 words per minute (wpm), 68% exceeded this limit at 0.5 logRAD, and 92% exceeded it at 0.6 logRAD. The mean reading speed at 0.5 logRAD was 134.76 ± 48.22 wpm; with reading glasses it was 167.65 ± 32.77 wpm (P < .05). A considerable percentage of patients with monofocal IOLs read newspaper print size without glasses under good light conditions. Copyright © 2014 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.

Dim-light photoreceptor of chub mackerel Scomber japonicus and the photoresponse upon illumination with LEDs of different wavelengths.

PubMed

Jang, Jun-Chul; Choi, Mi-Jin; Yang, Yong-Soo; Lee, Hyung-Been; Yu, Young-Moon; Kim, Jong-Myoung

2016-06-01

To study the absorption characteristics of rhodopsin, a dim-light photoreceptor, in chub mackerel (Scomber japonicus) and the relationship between light wavelengths on the photoresponse, the rod opsin gene was cloned into an expression vector, pMT4. Recombinant opsin was transiently expressed in COS-1 cells and reconstituted with 11-cis-retinal. Cells containing the regenerated rhodopsin were solubilized and subjected to UV/Vis spectroscopic analysis in the dark and upon illumination. Difference spectra from the lysates indicated an absorption maximum of mackerel rhodopsin around 500 nm. Four types of light-emitting diode (LED) modules with different wavelengths (red, peak 627 nm; cyan, 505 nm; blue, 442 nm; white, 447 + 560 nm) were constructed to examine their effects on the photoresponse in chub mackerel. Behavioral responses of the mackerels, including speed and frequencies acclimated in the dark and upon LED illumination, were analyzed using an underwater acoustic camera. Compared to an average speed of 22.25 ± 1.57 cm/s of mackerel movement in the dark, speed increased to 22.97 ± 0.29, 24.66 ± 1.06, 26.28 ± 2.28, and 25.19 ± 1.91 cm/s upon exposure to red, blue, cyan, and white LEDs, respectively. There were increases of 103.48 ± 1.58, 109.37 ± 5.29, 118.48 ± 10.82, and 109.43 ± 3.92 %, respectively, in the relative speed of the fishes upon illumination with red, blue, cyan, and white LEDs compared with that in the dark (set at 100 %). Similar rate of wavelength-dependent responses was observed in a frequency analysis. These results indicate that an LED emitting a peak wavelength close to an absorption maximum of rhodopsin is more effective at eliciting a response to light.

Two eyes for two purposes: in situ evidence for asymmetric vision in the cockeyed squids Histioteuthis heteropsis and Stigmatoteuthis dofleini

PubMed Central

Robison, Bruce H.

2017-01-01

The light environment of the mesopelagic realm of the ocean changes with both depth and viewer orientation, and this has probably driven the high diversity of visual adaptations found among its inhabitants. The mesopelagic ‘cockeyed’ squids of family Histioteuthidae have unusual eyes, as the left and right eyes are dimorphic in size, shape and sometimes lens pigmentation. This dimorphism may be an adaptation to the two different sources of light in the mesopelagic realm, with the large eye oriented upward to view objects silhouetted against the dim, downwelling sunlight and the small eye oriented slightly downward to view bioluminescent point sources. We used in situ video footage from remotely operated vehicles in the Monterey Submarine Canyon to observe the orientation behaviour of 152 Histioteuthis heteropsis and nine Stigmatoteuthis dofleini. We found evidence for upward orientation in the large eye and slightly downward orientation in the small eye, which was facilitated by a tail-up oblique body orientation. We also found that 65% of adult H. heteropsis (n = 69) had yellow pigmentation in the lens of the larger left eye, which may be used to break the counterillumination camouflage of their prey. Finally, we used visual modelling to show that the visual returns provided by increasing eye size are much higher for an upward-oriented eye than for a downward-oriented eye, which may explain the development of this unique visual strategy. This article is part of the themed issue ‘Vision in dim light’. PMID:28193814

Rearview Mirror Dimming Function

ERIC Educational Resources Information Center

Layton, William

2011-01-01

Students are often unaware of the little tab on a rear-view mirror that is used to dim headlights from the rear. Those who know about this tab are usually interested in knowing how it works. Explanations of the optics involved can be found in Serway and Jewett and Jones and Edge. An alternate explanation is given.

Bright-light exposure during daytime sleeping affects nocturnal melatonin secretion after simulated night work.

PubMed

Nagashima, Shunsuke; Osawa, Madoka; Matsuyama, Hiroto; Ohoka, Wataru; Ahn, Aemi; Wakamura, Tomoko

2018-02-01

The guidelines for night and shift workers recommend that after night work, they should sleep in a dark environment during the daytime. However, staying in a dark environment during the daytime reduces nocturnal melatonin secretion and delays its onset. Daytime bright-light exposure after night work is important for melatonin synthesis the subsequent night and for maintaining the circadian rhythms. However, it is not clear whether daytime sleeping after night work should be in a dim- or a bright-light environment for maintaining melatonin secretion. The aim of this study, therefore, was to evaluate the effect of bright-light exposure during daytime sleeping on nocturnal melatonin secretion after simulated night work. Twelve healthy male subjects, aged 24.8 ± 4.6 (mean ± SD), participated in 3-day sessions under two experimental conditions, bright light or dim light, in a random order. On the first day, the subjects entered the experimental room at 16:00 and saliva samples were collected every hour between 18:00 and 00:00 under dim-light conditions. Between 00:00 and 08:00, they participated in tasks that simulated night work. At 10:00 the next morning, they slept for 6 hours under either a bright-light condition (>3000 lx) or a dim-light condition (<50 lx). In the evening, saliva samples were collected as on the first day. The saliva samples were analyzed for melatonin concentration. Activity and sleep times were recorded by a wrist device worn throughout the experiment. In the statistical analysis, the time courses of melatonin concentration were compared between the two conditions by three-way repeated measurements ANOVA (light condition, day and time of day). The change in dim light melatonin onset (ΔDLMO) between the first and second days, and daytime and nocturnal sleep parameters after the simulated night work were compared between the light conditions using paired t-tests. The ANOVA results indicated a significant interaction (light condition and3

Chronic dim light at night provokes reversible depression-like phenotype: possible role for TNF.

PubMed

Bedrosian, T A; Weil, Z M; Nelson, R J

2013-08-01

The prevalence of major depression has increased in recent decades and women are twice as likely as men to develop the disorder. Recent environmental changes almost certainly have a role in this phenomenon, but a complete set of contributors remains unspecified. Exposure to artificial light at night (LAN) has surged in prevalence during the past 50 years, coinciding with rising rates of depression. Chronic exposure to LAN is linked to increased risk of breast cancer, obesity and mood disorders, although the relationship to mood is not well characterized. In this study, we investigated the effects of chronic exposure to 5 lux LAN on depression-like behaviors in female hamsters. Using this model, we also characterized hippocampal brain-derived neurotrophic factor expression and hippocampal dendritic morphology, and investigated the reversibility of these changes 1, 2 or 4 weeks following elimination of LAN. Furthermore, we explored the mechanism of action, focusing on hippocampal proinflammatory cytokines given their dual role in synaptic plasticity and the pathogenesis of depression. Using reverse transcription-quantitative PCR, we identified a reversible increase in hippocampal tumor necrosis factor (TNF), but not interleukin-1β, mRNA expression in hamsters exposed to LAN. Direct intracerebroventricular infusion of a dominant-negative inhibitor of soluble TNF, XPro1595, prevented the development of depression-like behavior under LAN, but had no effect on dendritic spine density in the hippocampus. These results indicate a partial role for TNF in the reversible depression-like phenotype observed under chronic dim LAN. Recent environmental changes, such as LAN exposure, may warrant more attention as possible contributors to rising rates of mood disorders.

A Survey of Coronal Dimmings and EIT Wave Transients

NASA Technical Reports Server (NTRS)

Thompson, Barbara J.

2003-01-01

We present the results of a comprehensive catalog of EIT wave transients and coronal dimmings. We will compiled a set of more than 170 events, and we present strong evidence for the association of the co-development of coronal dimmings and EIT waves. Both limb and disk events are included in this study. We also include the speeds, locations, and associated flare timing in this study.

Tools for controlling protein interactions with light

PubMed Central

Tucker, Chandra L.; Vrana, Justin D.; Kennedy, Matthew J.

2014-01-01

Genetically-encoded actuators that allow control of protein-protein interactions with light, termed ‘optical dimerizers’, are emerging as new tools for experimental biology. In recent years, numerous new and versatile dimerizer systems have been developed. Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and coordinate use of imaging reporters. We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use controlling transcription, protein localization, and protein secretion with light. Additionally, we provide instructions and software for constructing a pulse-controlled LED light device for use in experiments requiring extended light treatments. PMID:25181301

The Sensitivity of Clinical Outcomes to Centration on the Light-Constricted Pupil for a Shape-Changing Corneal Inlay.

PubMed

Barragán-Garza, Enrique; Koch, Douglas D; Vargas, Luis G; Lang, Alan; Roy, Adam

2018-03-01

To assess the clinically acceptable range of inlay decentration with respect to the light-constricted pupil center and the coaxially sighted corneal light reflex (CSCLR) for an inlay (Raindrop Near Vision Inlay; ReVision Optics, Inc., Lake Forest, CA) that reshapes the anterior corneal surface. In this retrospective, observational cohort study of 115 patients with emmetropic or low hyperopic presbyopia who were implanted with a shape-changing corneal inlay, visual acuity, task performance (in good and dim light), reports of halos and glare, and satisfaction data were collected from the preoperative and 3-month postoperative examinations. Inlay centration with respect to the pupil center and CSCLR was determined from the center of the inlay effect derived from iTrace (Tracey Technologies, Houston, TX) wavefront measurements. Multivariate regression models assessed the influence of inlay position on visual outcomes. On average, monocular uncorrected near visual acuity (UNVA) improved 4.9 ± 1.7 lines in the treated eye, with no loss in binocular distance vision. Eighty-three percent of implants were centered radially within 0.5 mm of the pupil center. Multivariate analysis of decentration with respect to both the pupil center and CSCLR revealed no significant interaction with the above clinical outcomes, with the exception of UNVA in the treated eye (all P > .05, α = 0.05). For decentration of less than 0.75 mm, the change in UNVA was less than 1 line. Distance and near visual acuity, task performance, severity of halos and glare, and satisfaction were independent of radial decentration of the Raindrop Near Vision Inlay of less than 0.75 mm from the light-constricted pupil. [J Refract Surg. 2018;34(3):164-170.]. Copyright 2018, SLACK Incorporated.

Light-Induced Changes of the Circadian Clock of Humans: Increasing Duration is More Effective than Increasing Light Intensity

PubMed Central

Dewan, Karuna; Benloucif, Susan; Reid, Kathryn; Wolfe, Lisa F.; Zee, Phyllis C.

2011-01-01

Study Objectives: To evaluate the effect of increasing the intensity and/or duration of exposure on light-induced changes in the timing of the circadian clock of humans. Design: Multifactorial randomized controlled trial, between and within subject design Setting: General Clinical Research Center (GCRC) of an academic medical center Participants: 56 healthy young subjects (20-40 years of age) Interventions: Research subjects were admitted for 2 independent stays of 4 nights/3 days for treatment with bright or dim-light (randomized order) at a time known to induce phase delays in circadian timing. The intensity and duration of the bright light were determined by random assignment to one of 9 treatment conditions (duration of 1, 2, or 3 hours at 2000, 4000, or 8000 lux). Measurements and Results: Treatment-induced changes in the dim light melatonin onset (DLMO) and dim light melatonin offset (DLMOff) were measured from blood samples collected every 20-30 min throughout baseline and post-treatment nights. Comparison by multi-factor analysis of variance (ANOVA) of light-induced changes in the time of the circadian melatonin rhythm for the 9 conditions revealed that changing the duration of the light exposure from 1 to 3 h increased the magnitude of light-induced delays. In contrast, increasing from moderate (2,000 lux) to high (8,000 lux) intensity light did not alter the magnitude of phase delays of the circadian melatonin rhythm. Conclusions: Results from the present study suggest that for phototherapy of circadian rhythm sleep disorders in humans, a longer period of moderate intensity light may be more effective than a shorter exposure period of high intensity light. Citation: Dewan K; Benloucif S; Reid K; Wolfe LF; Zee PC. Light-induced changes of the circadian clock of humans: increasing duration is more effective than increasing light intensity. SLEEP 2011;34(5):593-599. PMID:21532952

The bright-nights and dim-days of the urban photoperiod: implications for circadian rhythmicity, metabolism and obesity.

PubMed

Wyse, Cathy A; Biello, Stephany M; Gill, Jason M R

2014-08-01

Artificial light decreases the amplitude of daily rhythms in human lifestyle principally by permitting activity and food intake to occur during hours of darkness, and allowing day-time activity to occur in dim light, indoors. Endogenous circadian timing mechanisms that oscillate with a period of 24 h have evolved to ensure physiology is synchronized with the daily variations in light, food, and social cues of the environment. Artificial light affects the synchronization between these oscillators, and metabolic disruption may be one consequence of this. By dampening the amplitude of environmental timing cues and disrupting circadian rhythmicity, artificial lighting might initiate metabolic disruption and contribute to the association between global urbanization and obesity. The aim of this review is to explore the historical, physiological, and epidemiological relationships between artificial light and circadian and metabolic dysfunction.

Light-induced protein degradation in human-derived cells.

PubMed

Sun, Wansheng; Zhang, Wenyao; Zhang, Chao; Mao, Miaowei; Zhao, Yuzheng; Chen, Xianjun; Yang, Yi

2017-05-27

Controlling protein degradation can be a valuable tool for posttranslational regulation of protein abundance to study complex biological systems. In the present study, we designed a light-switchable degron consisting of a light oxygen voltage (LOV) domain of Avena sativa phototropin 1 (AsLOV2) and a C-terminal degron. Our results showed that the light-switchable degron could be used for rapid and specific induction of protein degradation in HEK293 cells by light in a proteasome-dependent manner. Further studies showed that the light-switchable degron could also be utilized to mediate the degradation of secreted Gaussia princeps luciferase (GLuc), demonstrating the adaptability of the light-switchable degron in different types of protein. We suggest that the light-switchable degron offers a robust tool to control protein levels and may serves as a new and significant method for gene- and cell-based therapies. Copyright © 2017 Elsevier Inc. All rights reserved.

Penguin colony attendance under artificial lights for ecotourism.

PubMed

Rodríguez, Airam; Holmberg, Ross; Dann, Peter; Chiaradia, André

2018-03-30

Wildlife watching is an emerging ecotourism activity around the world. In Australia and New Zealand, night viewing of little penguins attracts hundreds of thousands of visitors per year. As penguins start coming ashore after sunset, artificial lighting is essential to allow visitors to view them in the dark. This alteration of the nightscape warrants investigation for any potential effects of artificial lighting on penguin behavior. We experimentally tested how penguins respond to different light wavelengths (colors) and intensities to examine effects on the colony attendance behavior at two sites on Phillip Island, Australia. At one site, nocturnal artificial illumination has been used for penguin viewing for decades, whereas at the other site, the only light is from the natural night sky. Light intensity did not affect colony attendance behaviors of penguins at the artificially lit site, probably due to penguin habituation to lights. At the not previously lit site, penguins preferred lit paths over dark paths to reach their nests. Thus, artificial light might enhance penguin vision at night and consequently it might reduce predation risk and energetic costs of locomotion through obstacle and path detection. Although penguins are faithful to their path, they can be drawn to artificial lights at small spatial scale, so light pollution could attract penguins to undesirable lit areas. When artificial lighting is required, we recommend keeping lighting as dim and time-restricted as possible to mitigate any negative effects on the behavior of penguins and their natural habitat. © 2018 Wiley Periodicals, Inc.

Superior visual performance in nocturnal insects: neural principles and bio-inspired technologies

NASA Astrophysics Data System (ADS)

Warrant, Eric J.

2016-04-01

At night, our visual capacities are severely reduced, with a complete loss in our ability to see colour and a dramatic loss in our ability to see fine spatial and temporal details. This is not the case for many nocturnal animals, notably insects. Our recent work, particularly on fast-flying moths and bees and on ball-rolling dung beetles, has shown that nocturnal animals are able to distinguish colours, to detect faint movements, to learn visual landmarks, to orient to the faint pattern of polarised light produced by the moon and to navigate using the stars. These impressive visual abilities are the result of exquisitely adapted eyes and visual systems, the product of millions of years of evolution. Nocturnal animals typically have highly sensitive eye designs and visual neural circuitry that is optimised for extracting reliable information from dim and noisy visual images. Even though we are only at the threshold of understanding the neural mechanisms responsible for reliable nocturnal vision, growing evidence suggests that the neural summation of photons in space and time is critically important: even though vision in dim light becomes necessarily coarser and slower, it also becomes significantly more reliable. We explored the benefits of spatiotemporal summation by creating a computer algorithm that mimicked nocturnal visual processing strategies. This algorithm dramatically increased the reliability of video collected in dim light, including the preservation of colour, strengthening evidence that summation strategies are essential for nocturnal vision.

Extinction and the Dimming of KIC 8462852

NASA Astrophysics Data System (ADS)

Meng, Huan Y. A.; Rieke, George; Dubois, Franky; Kennedy, Grant; Marengo, Massimo; Siegel, Michael; Su, Kate; Trueba, Nicolas; Wyatt, Mark; Boyajian, Tabetha; Lisse, C. M.; Logie, Ludwig; Rau, Steve; Vanaverbeke, Sigfried

2017-10-01

To test alternative hypotheses for the behavior of KIC 8462852, we obtained measurements of the star over a wide wavelength range from the UV to the mid-infrared from 2015 October through 2016 December, using Swift, Spitzer and AstroLAB IRIS. The star faded in a manner similar to the long-term fading seen in Kepler data about 1400 days previously. The dimming rate for the entire period reported is 22.1 ± 9.7 mmag yr-1 in the Swift wavebands, with amounts of 21.0 ± 4.5 mmag in the ground-based B measurements, 14.0 ± 4.5 mmag in V, and 13.0 ± 4.5 in R, and a rate of 5.0 ± 1.2 mmag yr-1 averaged over the two warm Spitzer bands. Although the dimming is small, it is seen at ≳3σ by three different observatories operating from the UV to the IR. The presence of long-term secular dimming means that previous spectral energy distribution models of the star based on photometric measurements taken years apart may not be accurate. We find that stellar models with {T}{eff}=7000{--}7100 K and {A}V˜ 0.73 best fit the Swift data from UV to optical. These models also show no excess in the near-simultaneous Spitzer photometry at 3.6 and 4.5 μm, although a longer wavelength excess from a substantial debris disk is still possible (e.g., as around Fomalhaut). The wavelength dependence of the fading favors a relatively neutral color (I.e., {R}V≳ 5, but not flat across all the bands) compared with the extinction law for the general interstellar medium ({R}V=3.1), suggesting that the dimming arises from circumstellar material.

New lighting and controls to save 67% at mall

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kennedy, K.

1985-06-17

A metal halide lighting system and a Staco control system that dims lighting in response to sunlight should save a Dallas hotel and office mall 67% in electricity costs. The new system replaces quartz lamps in the 160-foot-high atrium. The savings are a combination of state tax credits and lower air conditioning costs, which will pay for the $25,000 project in about 18 months. The metal halide system was chosen over sodium lighting in order to have more attractive color rendition and because it provides about twice as many lumens per watt as the quartz lamps. The Staco system willmore » dim lamps in response to outdoor light and turn lights above a skating rink on and off at prescribed times.« less

Rod electrical coupling is controlled by a circadian clock and dopamine in mouse retina

PubMed Central

Jin, Nan Ge; Chuang, Alice Z; Masson, Philippe J; Ribelayga, Christophe P

2015-01-01

Key points Rod photoreceptors play a key role in vision in dim light; in the mammalian retina, although rods are anatomically connected or coupled by gap junctions, a type of electrical synapse, the functional importance and regulation of rod coupling has remained elusive. We have developed a new technique in the mouse: perforated patch-clamp recording of rod inner segments in isolated intact retinae maintained by superfusion. We find that rod electrical coupling is controlled by a circadian clock and dopamine, and is weak during the day and stronger at night. The results also indicate that the signal-to-noise ratio for a dim light response is increased at night because of coupling. Our observations will provide a framework for understanding the daily variations in human vision as well as the basis of specific retinal malfunctions. Abstract Rod single-photon responses are critical for vision in dim light. Electrical coupling via gap junction channels shapes the light response properties of vertebrate photoreceptors, but the regulation of rod coupling and its impact on the single-photon response have remained unclear. To directly address these questions, we developed a perforated patch-clamp recording technique and recorded from single rod inner segments in isolated intact neural mouse retinae, maintained by superfusion. Experiments were conducted at different times of the day or under constant environmental conditions, at different times across the circadian cycle. We show that rod electrical coupling is regulated by a circadian clock and dopamine, so that coupling is weak during the day and strong at night. Altogether, patch-clamp recordings of single-photon responses in mouse rods, tracer coupling, receptive field measurements and pharmacological manipulations of gap junction and dopamine receptor activity provide compelling evidence that rod coupling is modulated in a circadian manner. These data are consistent with computer modelling. At night, single

Comparing the Morningness-Eveningness Questionnaire and Munich ChronoType Questionnaire to the Dim Light Melatonin Onset.

PubMed

Kantermann, Thomas; Sung, Haein; Burgess, Helen J

2015-10-01

The dim light melatonin onset (DLMO) is the most reliable measure of central circadian timing in humans. However, it is not always possible to measure the DLMO because sample collection has to occur in the hours before usual sleep onset, it requires staff support and considerable participant effort, and it is relatively expensive. Questionnaires that ask people about the timing of their behavior, such as their sleep, may provide an easier and less expensive estimate of circadian timing. The objective of this analysis was to compare the MEQ score derived from the Morningness-Eveningness Questionnaire (MEQ) and the MSFsc derived from the Munich ChronoType Questionnaire (MCTQ) to the DLMO in the largest sample to date (N = 60). Our hypothesis was that MSFsc would correlate more highly with the DLMO than MEQ score. Our sample of 36 healthy controls and 24 patients with delayed sleep phase disorder ranged in age from 18 to 62 years. All participants slept at times of their own choosing for a week before the assessment of their DLMO. The DLMO correlated significantly with both the MEQ score (r = -0.70, p < 0.001) and MSFsc (r = 0.68, p < 0.001). A linear regression using MEQ, MSFsc, and age to predict the DLMO explained 60% of the DLMO variance. The strongest predictor of the DLMO was MSFsc (beta = 0.51, p = 0.001), followed by MEQ (beta = -0.41, p = 0.004), and age (beta = 0.26, p = 0.013). The beta values for MSFsc and MEQ score were not statistically different from each other. Nonetheless, around a 4-h range in the DLMO was observed at a given MEQ score and a given MSFsc, indicating that neither questionnaire should be exclusively used to time light or exogenous melatonin treatment, as this could result in the mistiming of these treatments relative to the DLMO, thereby potentially worsening circadian misalignment. © 2015 The Author(s).

Non-grey dimming events of KIC 8462852 from GTC spectrophotometry

NASA Astrophysics Data System (ADS)

Deeg, Hans J.; Alonso, Roi; Nespral, David; Boyajian, Tabetha S.

2018-02-01

We report ground-based spectrophotometry of KIC 8462852 obtained during its first dimming events since the end of the Kepler mission. The dimmings show a clear colour signature and are deeper in visual blue than in red wavelengths. The wavelength dependency of the flux loss can be described with an absorption Ångström coefficient of 2.19 ± 0.45, which is compatible with absorption by optically thin dust with particle sizes of the order of 0.0015 to 0.15 μm. These particles would be smaller than is required to be resistant against blow-out by radiation pressure when close to the star. During occultation events, these particles must be replenished from a comoving body on time-scales of days. If dust is indeed the source of the dimming events of KIC 8462852, deeper dimming events should show more neutral colours, as is expected from optically thick absorbers. The individual reduced spectra of the target and reference stars are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/610/L12

Human Adolescent Phase Response Curves to Bright White Light.

PubMed

Crowley, Stephanie J; Eastman, Charmane I

2017-08-01

Older adolescents are particularly vulnerable to circadian misalignment and sleep restriction, primarily due to early school start times. Light can shift the circadian system and could help attenuate circadian misalignment; however, a phase response curve (PRC) to determine the optimal time for receiving light and avoiding light is not available for adolescents. We constructed light PRCs for late pubertal to postpubertal adolescents aged 14 to 17 years. Participants completed 2 counterbalanced 5-day laboratory sessions after 8 or 9 days of scheduled sleep at home. Each session included phase assessments to measure the dim light melatonin onset (DLMO) before and after 3 days of free-running through an ultradian light-dark (wake-sleep) cycle (2 h dim [~20 lux] light, 2 h dark). In one session, intermittent bright white light (~5000 lux; four 20-min exposures) was alternated with 10 min of dim room light once per day for 3 consecutive days. The time of light varied among participants to cover the 24-h day. For each individual, the phase shift to bright light was corrected for the free-run derived from the other laboratory session with no bright light. One PRC showed phase shifts in response to light start time relative to the DLMO and another relative to home sleep. Phase delay shifts occurred around the hours corresponding to home bedtime. Phase advances occurred during the hours surrounding wake time and later in the afternoon. The transition from delays to advances occurred at the midpoint of home sleep. The adolescent PRCs presented here provide a valuable tool to time bright light in adolescents.

Mechanisms and Observations of Coronal Dimming for the 2010 August 7 Event

NASA Technical Reports Server (NTRS)

Mason, James P.; Woods, Thomas N.; Caspi, Amir; Thompson, Barbara J.; Hock, Rachel A.

2014-01-01

Coronal dimming of extreme ultraviolet (EUV) emission has the potential to be a useful forecaster of coronal mass ejections (CMEs). As emitting material leaves the corona, a temporary void is left behind which can be observed in spectral images and irradiance measurements. The velocity and mass of the CMEs should impact the character of those observations. However, other physical processes can confuse the observations. We describe these processes and the expected observational signature, with special emphasis placed on the differences. We then apply this understanding to a coronal dimming event with an associated CME that occurred on 2010 August 7. Data from the Solar Dynamics Observatory's (SDO) Atmospheric Imaging Assembly (AIA) and EUV Variability Experiment (EVE) are used for observations of the dimming, while the Solar and Heliospheric Observatory's (SoHO) Large Angle and Spectrometric Coronagraph (LASCO) and the Solar Terrestrial Relations Observatory's (STEREO) COR1 and COR2 are used to obtain velocity and mass estimates for the associated CME. We develop a technique for mitigating temperature effects in coronal dimming from full-disk irradiance measurements taken by EVE. We find that for this event, nearly 100% of the dimming is due to mass loss in the corona.

Mechanisms and observations of coronal dimming for the 201 August 7 event

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mason, James Paul; Woods, T. N.; Caspi, A.

2014-07-01

Coronal dimming of extreme ultraviolet (EUV) emission has the potential to be a useful forecaster of coronal mass ejections (CMEs). As emitting material leaves the corona, a temporary void is left behind which can be observed in spectral images and irradiance measurements. The velocity and mass of the CMEs should impact the character of those observations. However, other physical processes can confuse the observations. We describe these processes and the expected observational signature, with special emphasis placed on the differences. We then apply this understanding to a coronal dimming event with an associated CME that occurred on 2010 August 7.more » Data from the Solar Dynamics Observatory's Atmospheric Imaging Assembly and EUV Variability Experiment (EVE) are used for observations of the dimming, while the Solar and Heliospheric Observatory's Large Angle and Spectrometric Coronagraph and the Solar Terrestrial Relations Observatory's COR1 and COR2 are used to obtain velocity and mass estimates for the associated CME. We develop a technique for mitigating temperature effects in coronal dimming from full-disk irradiance measurements taken by EVE. We find that for this event, nearly 100% of the dimming is due to mass loss in the corona.« less

What aspects of vision facilitate haptic processing?

PubMed

Millar, Susanna; Al-Attar, Zainab

2005-12-01

We investigate how vision affects haptic performance when task-relevant visual cues are reduced or excluded. The task was to remember the spatial location of six landmarks that were explored by touch in a tactile map. Here, we use specially designed spectacles that simulate residual peripheral vision, tunnel vision, diffuse light perception, and total blindness. Results for target locations differed, suggesting additional effects from adjacent touch cues. These are discussed. Touch with full vision was most accurate, as expected. Peripheral and tunnel vision, which reduce visuo-spatial cues, differed in error pattern. Both were less accurate than full vision, and significantly more accurate than touch with diffuse light perception, and touch alone. The important finding was that touch with diffuse light perception, which excludes spatial cues, did not differ from touch without vision in performance accuracy, nor in location error pattern. The contrast between spatially relevant versus spatially irrelevant vision provides new, rather decisive, evidence against the hypothesis that vision affects haptic processing even if it does not add task-relevant information. The results support optimal integration theories, and suggest that spatial and non-spatial aspects of vision need explicit distinction in bimodal studies and theories of spatial integration.

Short nights reduce light-induced circadian phase delays in humans.

PubMed

Burgess, Helen J; Eastman, Charmane I

2006-01-01

Short sleep episodes are common in modern society. We recently demonstrated that short nights reduce phase advances to light. Here we show that short nights also reduce phase delays to light. Two weeks of 6-hour sleep episodes in the dark (short nights) and 2 weeks of long 9-hour sleep episodes (long nights) in counterbalanced order, separated by 7 days. Following each series of nights, there was a dim-light phase assessment to assess baseline phase. Three days later, subjects were exposed to a phase-delaying light stimulus for 2 days, followed by a final phase assessment. Subjects slept at home in dark bedrooms but came to the laboratory for the phase assessments and light stimulus. Seven young healthy subjects. The 3.5-hour light stimulus was four 30-minute pulses of bright light (-5000 lux) separated by 30-minute intervals of room light. The stimulus began 2.5 hours after each subject's dim-light melatonin onset, followed by a 6- or 9-hour sleep episode. On the second night, the bright light and sleep episode began 1 hour later. The dim-light melatonin onset and dimlight melatonin offset phase delayed 1.4 and 0.7 hours less in the short nights, respectively (both p < or = .015). These results indicate for the first time that short nights can reduce circadian phase delays, that long nights can increase phase delays to light, or both. People who curtail their sleep may inadvertently reduce their circadian responsiveness to evening light.

Dimming LEDs with Phase-Cut Dimmers. The Specifier's Process for Maximizing Success

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, N. J.; Poplawski, M. E.

2013-10-01

DOE GATEWAY program report reviewing how phase-cut dimmers work, how LEDs differ from the incandescent lamps these dimmers were originally designed to control, and how those differences can lead to complications when attempting to dim LEDs. Providing both general guidance and step-by-step procedures for designing phase-controlled LED dimming on both new and existing projects—as well as real-world examples of how to use those procedures—the report aims to reduce the chance of experiencing compatibility-related problems and, if possible, ensure good dimming performance.

Functional Accumulation of Antenna Proteins in Chlorophyll b-Less Mutants of Chlamydomonas reinhardtii.

PubMed

Bujaldon, Sandrine; Kodama, Natsumi; Rappaport, Fabrice; Subramanyam, Rajagopal; de Vitry, Catherine; Takahashi, Yuichiro; Wollman, Francis-André

2017-01-09

The green alga Chlamydomonas reinhardtii contains several light-harvesting chlorophyll a/b complexes (LHC): four major LHCIIs, two minor LHCIIs, and nine LHCIs. We characterized three chlorophyll b-less mutants to assess the effect of chlorophyll b deficiency on the function, assembly, and stability of these chlorophyll a/b binding proteins. We identified point mutations in two mutants that inactivate the CAO gene responsible for chlorophyll a to chlorophyll b conversion. All LHCIIs accumulated to wild-type levels in a CAO mutant but their light-harvesting function for photosystem II was impaired. In contrast, most LHCIs accumulated to wild-type levels in the mutant and their light-harvesting capability for photosystem I remained unaltered. Unexpectedly, LHCI accumulation and the photosystem I functional antenna size increased in the mutant compared with in the wild type when grown in dim light. When the CAO mutation was placed in a yellow-in-the-dark background (yid-BF3), in which chlorophyll a synthesis remains limited in dim light, accumulation of the major LHCIIs and of most LHCIs was markedly reduced, indicating that sustained synthesis of chlorophyll a is required to preserve the proteolytic resistance of antenna proteins. Indeed, after crossing yid-BF3 with a mutant defective for the thylakoid FtsH protease activity, yid-BF3-ftsh1 restored wild-type levels of LHCI, which defines LHCI as a new substrate for the FtsH protease. Copyright © 2017 The Author. Published by Elsevier Inc. All rights reserved.

Sex and vision II: color appearance of monochromatic lights.

PubMed

Abramov, Israel; Gordon, James; Feldman, Olga; Chavarga, Alla

2012-09-04

Because cerebral cortex has a very large number of testosterone receptors, we examined the possible sex differences in color appearance of monochromatic lights across the visible spectrum. There is a history of men and women perceiving color differently. However, all of these studies deal with higher cognitive functions which may be culture-biased. We study basic visual functions, such as color appearance, without reference to any objects. We present here a detailed analysis of sex differences in primary chromatic sensations. We tested large groups of young adults with normal vision, including spatial and temporal resolution, and stereopsis. Based on standard color-screening and anomaloscope data, we excluded all color-deficient observers. Stimuli were equi-luminant monochromatic lights across the spectrum. They were foveally-viewed flashes presented against a dark background. The elicited sensations were measured using magnitude estimation of hue and saturation. When the only permitted hue terms are red (R) yellow (Y), green (G), blue (B), alone or in combination, such hue descriptions are language-independent and the hue and saturation values can be used to derive a wide range of color-discrimination functions. There were relatively small but clear and significant, differences between males and females in the hue sensations elicited by almost the entire spectrum. Generally, males required a slightly longer wavelength to experience the same hue as did females. The spectral loci of the unique hues are not correlated with anomaloscope matches; these matches are directly determined by the spectral sensitivities of L- and M-cones (genes for these cones are on the X-chromosomes). Nor are there correlations between loci of pairs of unique hues (R, Y, G, B). Wavelength-discrimination functions derived from the scaling data show that males have a broader range of poorer discrimination in the middle of the spectrum. The precise values for all the data depend on whether

Role of recoverin in rod photoreceptor light adaptation.

PubMed

Morshedian, Ala; Woodruff, Michael L; Fain, Gordon L

2018-04-15

Recoverin is a small molecular-weight, calcium-binding protein in rod outer segments that can modulate the rate of rhodopsin phosphorylation. We describe two additional and perhaps more important functions during photoreceptor light adaptation. Recoverin influences the rate of change of adaptation. In wild-type rods, sensitivity and response integration time adapt with similar time constants of 150-200 ms. In Rv-/- rods lacking recoverin, sensitivity declines faster and integration time is already shorter and not significantly altered. During steady light exposure, rod circulating current slowly increases during a time course of tens of seconds, gradually extending the operating range of the rod. In Rv-/- rods, this mechanism is deleted, steady-state currents are already larger and rods saturate at brighter intensities. We propose that recoverin modulates spontaneous and light-activated phophodiesterase-6, the phototransduction effector enzyme, to increase sensitivity in dim light but improve responsiveness to change in brighter illumination. Recoverin is a small molecular-weight, calcium-binding protein in rod outer segments that binds to G-protein receptor kinase 1 and can alter the rate of rhodopsin phosphorylation. A change in phosphorylation should change the lifetime of light-activated rhodopsin and the gain of phototransduction, but deletion of recoverin has little effect on the sensitivity of rods either in the dark or in dim-to-moderate background light. We describe two additional functions perhaps of greater physiological significance. (i) When the ambient intensity increases, sensitivity and integration time decrease in wild-type (WT) rods with similar time constants of 150-200 ms. Recoverin is part of the mechanism controlling this process because, in Rv-/- rods lacking recoverin, sensitivity declines more rapidly and integration time is already shorter and not further altered. (ii) During steady light exposure, WT rod circulating current slowly

Improving automated 3D reconstruction methods via vision metrology

NASA Astrophysics Data System (ADS)

Toschi, Isabella; Nocerino, Erica; Hess, Mona; Menna, Fabio; Sargeant, Ben; MacDonald, Lindsay; Remondino, Fabio; Robson, Stuart

2015-05-01

This paper aims to provide a procedure for improving automated 3D reconstruction methods via vision metrology. The 3D reconstruction problem is generally addressed using two different approaches. On the one hand, vision metrology (VM) systems try to accurately derive 3D coordinates of few sparse object points for industrial measurement and inspection applications; on the other, recent dense image matching (DIM) algorithms are designed to produce dense point clouds for surface representations and analyses. This paper strives to demonstrate a step towards narrowing the gap between traditional VM and DIM approaches. Efforts are therefore intended to (i) test the metric performance of the automated photogrammetric 3D reconstruction procedure, (ii) enhance the accuracy of the final results and (iii) obtain statistical indicators of the quality achieved in the orientation step. VM tools are exploited to integrate their main functionalities (centroid measurement, photogrammetric network adjustment, precision assessment, etc.) into the pipeline of 3D dense reconstruction. Finally, geometric analyses and accuracy evaluations are performed on the raw output of the matching (i.e. the point clouds) by adopting a metrological approach. The latter is based on the use of known geometric shapes and quality parameters derived from VDI/VDE guidelines. Tests are carried out by imaging the calibrated Portable Metric Test Object, designed and built at University College London (UCL), UK. It allows assessment of the performance of the image orientation and matching procedures within a typical industrial scenario, characterised by poor texture and known 3D/2D shapes.

Challenges in Physical Characterization of Dim Space Objects: What Can We Learn from NEOs

NASA Astrophysics Data System (ADS)

Reddy, V.; Sanchez, J.; Thirouin, A.; Rivera-Valentin, E.; Ryan, W.; Ryan, E.; Mokovitz, N.; Tegler, S.

2016-09-01

Physical characterization of dim space objects in cis-lunar space can be a challenging task. Of particular interest to both natural and artificial space object behavior scientists are the properties beyond orbital parameters that can uniquely identify them. These properties include rotational state, size, shape, density and composition. A wide range of observational and non-observational factors affect our ability to characterize dim objects in cis-lunar space. For example, phase angle (angle between Sun-Target-Observer), temperature, rotational variations, temperature, and particle size (for natural dim objects). Over the last two decades, space object behavior scientists studying natural dim objects have attempted to quantify and correct for a majority of these factors to enhance our situational awareness. These efforts have been primarily focused on developing laboratory spectral calibrations in a space-like environment. Calibrations developed correcting spectral observations of natural dim objects could be applied to characterizing artificial objects, as the underlying physics is the same. The paper will summarize our current understanding of these observational and non-observational factors and present a case study showcasing the state of the art in characterization of natural dim objects.

Effect of Light Intensity and Photoperiod on Growth and Biochemical Composition of a Local Isolate of Nostoc calcicola.

PubMed

Khajepour, Fateme; Hosseini, Seyed Abbas; Ghorbani Nasrabadi, Rasoul; Markou, Giorgos

2015-08-01

A study was conducted to investigate the effect of light intensity (21, 42, and 63 μmol photons m(-2) s(-1)) and photoperiod (8:16, 12:12, and 16:8 h light/dark) on the biomass production and its biochemical composition (total carotenoids, chlorophyll a, phycoerythrin (PE), phycocyanin (PC) and allophycocyanin (APC), total protein, and carbohydrates) of a local isolate of Nostoc calcicola. The results revealed that N. calcicola prefers dim light; however, the most of the levels of light intensity and photoperiod investigated did not have a significant impact on biomass production. Increasing light intensity biomass content of chlorophyll a, PE, PC, APC, and total protein decreased, while total carotenoids and carbohydrate increased. The same behavior was observed also when light duration (photoperiod) increased. The interaction effect of increasing light intensity and photoperiod resulted in an increase of carbohydrate and total carotenoids, and to the decrease of chlorophyll a, PE, PC, APC, and total protein content. The results indicate that varying the light regime, it is capable to manipulate the biochemical composition of the local isolate of N. calcicola, producing either valuable phycobiliproteins or proteins under low light intensity and shorter photoperiods, or producing carbohydrates and carotenoids under higher light intensities and longer photoperiods.

Light Up Their Lives: A Review of Research on the Effects of Lighting on Children's Achievement and Behavior.

ERIC Educational Resources Information Center

Dunn, Rita; And Others

1985-01-01

Cites research showing individual reactions to bright and dim light in the classroom. Shows individual susceptibility to extreme negativism in inappropriate lighting conditions and suggests that students' predispositions for illumination be identified. Notes that restless, fidgety youngsters should be placed into softly lit sections, with the…

Visual function of police officers who have undergone refractive surgery.

PubMed

Hovis, Jeffery K; Ramaswamy, Shankaran

2006-11-01

The visual acuity and contrast sensitivity of police recruits and officers was evaluated in both normal and dim illumination conditions to determine whether officers who have had refractive surgery have compromised night vision. The control group consisted of 76 officers and recruits who have not had refractive surgery and the refractive surgery group consisted of 22 officers and recruits who had refractive surgery. Visual acuity and contrast sensitivity were measured under both room illumination and dim illumination. The room illumination test series included high contrast acuity, low contrast acuity and Pelli-Robson contrast sensitivity. The dim illumination test series included high contrast acuity, low contrast acuity, Pelli-Robson contrast sensitivity, license plate number acuity (with and without glare) and the Mesotest. The general findings were that the refractive surgery group had lower acuity scores on low contrast targets in both room and dim light levels along with a reduction in the Mesotest scores with a glare source compared to the control group. Although refractive surgery police recruits and officers had reduced performance on some vision tests, these reductions were small and it is unlikely that their performance on vision related tasks would be compromised, on average. The major concern is the small number of refractive surgery candidates whose results were well outside the range of the non-surgical candidates. Their vision may be unacceptable for policing. Copyright (c) 2006 Wiley-Liss, Inc.

49 CFR 229.125 - Headlights and auxiliary lights.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Headlights and auxiliary lights. 229.125 Section... Cab Equipment § 229.125 Headlights and auxiliary lights. (a) Each lead locomotive used in road service.... (c) Headlights shall be provided with a device to dim the light. (d) Effective December 31, 1997...

49 CFR 229.125 - Headlights and auxiliary lights.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Headlights and auxiliary lights. 229.125 Section... Cab Equipment § 229.125 Headlights and auxiliary lights. (a) Each lead locomotive used in road service.... (c) Headlights shall be provided with a device to dim the light. (d) Effective December 31, 1997...

Evaluation of salivary melatonin measurements for Dim Light Melatonin Onset calculations in patients with possible sleep-wake rhythm disorders.

PubMed

Keijzer, Henry; Smits, Marcel G; Peeters, Twan; Looman, Caspar W N; Endenburg, Silvia C; Gunnewiek, Jacqueline M T Klein

2011-08-17

Dim Light Melatonin Onset (DLMO) can be calculated within a 5-point partial melatonin curve in saliva collected at home. We retrospectively analyzed the patient melatonin measurements sample size of the year 2008 to evaluate these DLMO calculations and studied the correlation between diary or polysomnography (PSG) sleep onset and DLMO. Patients completed an online questionnaire. If this questionnaire pointed to a possible Delayed Sleep Phase Disorder (DSPD), saliva collection devices were sent to the patient. Collection occurred at 5 consecutive hours. Melatonin concentration was measured with a radioimmunoassay and DLMO was defined as the time at which the melatonin concentration in saliva reaches 4 pg/mL. Sleep onset time was retrieved from an online one-week sleep diary and/or one-night PSG. A total of 1848 diagnostic 5-point curves were obtained. DLMO could be determined in 76.2% (n=1408). DLMO significantly differed between different age groups and increased with age. Pearson correlations (r) between DLMO and sleep onset measured with PSG or with a diary were 0.514 (p=<0.001, n=54) and 0.653 (p=0.002, n=20) respectively. DLMO can be reliably measured in saliva that is conveniently collected at home. DLMO correlates moderately with sleep onset. Copyright © 2011 Elsevier B.V. All rights reserved.

Influence of light at night on melatonin suppression in children.

PubMed

Higuchi, Shigekazu; Nagafuchi, Yuki; Lee, Sang-il; Harada, Tetsuo

2014-09-01

The sensitivity of melatonin to light suppression is expected to be higher in children because children have large pupils and pure crystal lenses. However, melatonin suppression by light in children remains unclear. We investigated whether light-induced melatonin suppression in children is larger than that in adults. Thirty-three healthy primary school children (mean age, 9.2 ± 1.5 y) and 29 healthy adults (mean age, 41.6 ± 4.7 y) participated in two experiments. In the first experiment, salivary melatonin concentrations in 13 children and 13 adults were measured at night under a dim light (<30 lux) and a moderately bright light (580 lux) in an experimental facility. Pupil diameters were also measured under dim light and bright light. In the second experiment, melatonin concentrations in 20 children and 16 adults were measured under dim light in the experimental facility and under room light at home (illuminance, 140.0 ± 82.7 lux). In experiment 1, the melatonin concentration was significantly decreased by exposure to moderately bright light in both adults and children. Melatonin suppression was significantly larger in children (88.2%; n = 5) than in adults (46.3%; n = 6; P < .01), although the data for some participants were excluded because melatonin concentrations had not yet risen. In experiment 2, melatonin secretion was significantly suppressed by room light at home in children (n = 15; P < .05) but not in adults (n = 11). We found that the percentage of melatonin suppression by light in children was almost twice that in adults, suggesting that melatonin is more sensitive to light in children than in adults at night.

Night Vision Camera

NASA Technical Reports Server (NTRS)

1996-01-01

PixelVision, Inc. developed the Night Video NV652 Back-illuminated CCD Camera, based on the expertise of a former Jet Propulsion Laboratory employee and a former employee of Scientific Imaging Technologies, Inc. The camera operates without an image intensifier, using back-illuminated and thinned CCD technology to achieve extremely low light level imaging performance. The advantages of PixelVision's system over conventional cameras include greater resolution and better target identification under low light conditions, lower cost and a longer lifetime. It is used commercially for research and aviation.

A High Precision Approach to Calibrate a Structured Light Vision Sensor in a Robot-Based Three-Dimensional Measurement System.

PubMed

Wu, Defeng; Chen, Tianfei; Li, Aiguo

2016-08-30

A robot-based three-dimensional (3D) measurement system is presented. In the presented system, a structured light vision sensor is mounted on the arm of an industrial robot. Measurement accuracy is one of the most important aspects of any 3D measurement system. To improve the measuring accuracy of the structured light vision sensor, a novel sensor calibration approach is proposed to improve the calibration accuracy. The approach is based on a number of fixed concentric circles manufactured in a calibration target. The concentric circle is employed to determine the real projected centres of the circles. Then, a calibration point generation procedure is used with the help of the calibrated robot. When enough calibration points are ready, the radial alignment constraint (RAC) method is adopted to calibrate the camera model. A multilayer perceptron neural network (MLPNN) is then employed to identify the calibration residuals after the application of the RAC method. Therefore, the hybrid pinhole model and the MLPNN are used to represent the real camera model. Using a standard ball to validate the effectiveness of the presented technique, the experimental results demonstrate that the proposed novel calibration approach can achieve a highly accurate model of the structured light vision sensor.

Erratum: Correction to: Polarisation vision: overcoming challenges of working with a property of light we barely see

NASA Astrophysics Data System (ADS)

Foster, James J.; Temple, Shelby E.; How, Martin J.; Daly, Ilse M.; Sharkey, Camilla R.; Wilby, David; Roberts, Nicholas W.

2018-06-01

In "Polarisation vision: overcoming challenges of working with a property of light we barely see" (Foster et al. 2018) we provide a basic description of how Stokes parameters can be estimated and used to calculate the angle of polarisation (AoP).

Dark goggles and bright light improve circadian rhythm adaptation to night-shift work.

PubMed

Eastman, C I; Stewart, K T; Mahoney, M P; Liu, L; Fogg, L F

1994-09-01

We compared the contributions of bright light during the night shift and dark goggles during daylight for phase shifting the circadian rhythm of temperature to realign with a 12-hour shift of sleep. After 10 baseline days there were 8 night-work/day-sleep days. Temperature was continuously recorded from 50 subjects. There were four groups in a 2 x 2 design: light (bright, dim), goggles (yes, no). Subjects were exposed to bright light (about 5,000 lux) for 6 hours on the first 2 night shifts. Dim light was < 500 lux. Both bright light and goggles were significant factors for producing circadian rhythm phase shifts. The combination of bright light plus goggles was the most effective, whereas the combination of dim light and no goggles was the least effective. The temperature rhythm either phase advanced or phase delayed when it aligned with daytime sleep. However, when subjects did not have goggles only phase advances occurred. Goggles were necessary for producing phase delays. The most likely explanation is that daylight during the travel-home window after a night shift inhibits phase-delay shifts, and goggles can prevent this inhibition. Larger temperature-rhythm phase shifts were associated with better subjective daytime sleep, less subjective fatigue and better mood.

Restoration of vision in the pde6β-deficient dog, a large animal model of rod-cone dystrophy.

PubMed

Petit, Lolita; Lhériteau, Elsa; Weber, Michel; Le Meur, Guylène; Deschamps, Jack-Yves; Provost, Nathalie; Mendes-Madeira, Alexandra; Libeau, Lyse; Guihal, Caroline; Colle, Marie-Anne; Moullier, Philippe; Rolling, Fabienne

2012-11-01

Defects in the β subunit of rod cGMP phosphodiesterase 6 (PDE6β) are associated with autosomal recessive retinitis pigmentosa (RP), a childhood blinding disease with early retinal degeneration and vision loss. To date, there is no treatment for this pathology. The aim of this preclinical study was to test recombinant adeno-associated virus (AAV)-mediated gene addition therapy in the rod-cone dysplasia type 1 (rcd1) dog, a large animal model of naturally occurring PDE6β deficiency that strongly resembles the human pathology. A total of eight rcd1 dogs were injected subretinally with AAV2/5RK.cpde6β (n = 4) or AAV2/8RK.cpde6β (n = 4). In vivo and post-mortem morphological analysis showed a significant preservation of the retinal structure in transduced areas of both AAV2/5RK.cpde6β- and AAV2/8RK.cpde6β-treated retinas. Moreover, substantial rod-derived electroretinography (ERG) signals were recorded as soon as 1 month postinjection (35% of normal eyes) and remained stable for at least 18 months (the duration of the study) in treated eyes. Rod-responses were undetectable in untreated contralateral eyes. Most importantly, dim-light vision was restored in all treated rcd1 dogs. These results demonstrate for the first time that gene therapy effectively restores long-term retinal function and vision in a large animal model of autosomal recessive rod-cone dystrophy, and provide great promise for human treatment.

Growth and tuberization of potato (Solanum tuberosum L.) under continuous light

NASA Technical Reports Server (NTRS)

Wheeler, R. M.; Tibbitts, T. W.

1986-01-01

The growth and tuberization of potatoes (Solanum tuberosum L.) maintained for 6 weeks under four different regimes of continuous irradiance were compared to plants given 12 hours light and 12 hours dark. Treatments included: (a) continuous photosynthetic photon flux of 200 micromoles per square meter per second cool-white fluorescent (CWF); (b) continuous 400 micromoles per square meter per second CWF; (c) 12 hours 400 micromoles per square meter per second CWF plus 12 hours dim CWF at 5 micromoles per square meter per second; (d) 12 hours [400] micromoles per square meter per second CWF plus 12 hours dim incandescent (INC) at 5 micromoles per square meter per second and a control treatment of 12 hours light at 400 micromoles per square meter per second CWF and 12 hours dark. The study included five cultivars ranging from early- to late-season types: 'Norland,' 'Superior,''Norchip,' 'Russet Burbank,' and 'Kennebec,' Tuber development progressed well under continuous irradiation at 400 micromoles per square meter per second and under 12 hours irradiance and 12 hours dark, while tuber development was suppressed in all other light treatments. Continuous irradiation at 200 or 400 micromoles per square meter per second resulted in severe stunting and leaf malformation on 'Superior' and 'Kennebec' plants, but little or no injury and vigorous shoot growth in the other cultivars. No injury or stunting were apparent under 12-dim light or 12-dark treatments. Plants given 12 hours dim INC showed significantly greater stem elongation but less total biomass than plants in other treatments. The continuous light encouraged shoot growth over tuber growth but this trend was overridden by providing a high irradiance level. The variation among cultivars for tolerance to continuous lighting indicates that potato may be a useful species for photoinhibition studies.

Multi-camera and structured-light vision system (MSVS) for dynamic high-accuracy 3D measurements of railway tunnels.

PubMed

Zhan, Dong; Yu, Long; Xiao, Jian; Chen, Tanglong

2015-04-14

Railway tunnel 3D clearance inspection is critical to guaranteeing railway operation safety. However, it is a challenge to inspect railway tunnel 3D clearance using a vision system, because both the spatial range and field of view (FOV) of such measurements are quite large. This paper summarizes our work on dynamic railway tunnel 3D clearance inspection based on a multi-camera and structured-light vision system (MSVS). First, the configuration of the MSVS is described. Then, the global calibration for the MSVS is discussed in detail. The onboard vision system is mounted on a dedicated vehicle and is expected to suffer from multiple degrees of freedom vibrations brought about by the running vehicle. Any small vibration can result in substantial measurement errors. In order to overcome this problem, a vehicle motion deviation rectifying method is investigated. Experiments using the vision inspection system are conducted with satisfactory online measurement results.

Effects of an advanced sleep schedule and morning short wavelength light exposure on circadian phase in young adults with late sleep schedules.

PubMed

Sharkey, Katherine M; Carskadon, Mary A; Figueiro, Mariana G; Zhu, Yong; Rea, Mark S

2011-08-01

We examined the effects of an advanced sleep/wake schedule and morning short wavelength (blue) light in 25 adults (mean age±SD=21.8±3 years; 13 women) with late sleep schedules and subclinical features of delayed sleep phase disorder (DSPD). After a baseline week, participants kept individualized, fixed, advanced 7.5-h sleep schedules for 6days. Participants were randomly assigned to groups to receive "blue" (470nm, ∼225lux, n=12) or "dim" (<1lux, n=13) light for 1h after waking each day. Head-worn "Daysimeters" measured light exposure; actigraphs and sleep diaries confirmed schedule compliance. Salivary dim light melatonin onset (DLMO), self-reported sleep, and mood were examined with 2×2 ANOVA. After 6days, both groups showed significant circadian phase advances, but morning blue light was not associated with larger phase shifts than dim-light exposure. The average DLMO advances (mean±SD) were 1.5±1.1h in the dim light group and 1.4±0.7h in the blue light group. Adherence to a fixed advanced sleep/wake schedule resulted in significant circadian phase shifts in young adults with subclinical DSPD with or without morning blue light exposure. Light/dark exposures associated with fixed early sleep schedules are sufficient to advance circadian phase in young adults. Copyright © 2011 Elsevier B.V. All rights reserved.

Recent Global Dimming and Brightening and its causes from a satellite perspective

NASA Astrophysics Data System (ADS)

Ioannidis, Eleftherios; Papadimas, Christos D.; Benas, Nikolaos; Fotiadi, Aggeliki; Matsoukas, Christos; Hatzianastassiou, Nikolaos; Wild, Martin; Vardavas, Ilias M.

2017-04-01

, GDB is determined for the sub-period 2000-2009 using the same data as with the first case, whereas in the third case GDB is computed for 2000-2009 but using temporally varying aerosol properties from MODIS. The inter-comparison of GDB from the three case studies aims to shed light on the identification of the possible causes of the phenomenon, attempting to conclude whether or not clouds or aerosols are responsible and to what spatial and temporal extent. In all cases the model fluxes are evaluated through comparisons against reference BSRN (Baseline Surface Radiation Network) data. Preliminary results for the third case study, i.e. 2001-2009 using MODIS aerosol data, indicate apatchy global picture of GDB, yet with an overall dimming in the Northern Hemisphere (NH) equal to -2.3 W/m^2, and a stronger dimming of -4.15 W/m2 in the Southern Hemisphere (SH), thus suggesting an inter-hemispherical GDB difference. According to our analysis, clouds seem to be the most important cause of dimming in both hemispheres. Specifically, mid-level cloud cover, which increased by 7.4% in NH and 9.5% in SH, and cloud optical thickness for low, middle and high clouds, which increased by 5% in NH and 10-15% in SH depending on cloud type, appear to explain the post-2000 GDB and its inter-hemispherical differences.

Low Vision Aids in Glaucoma

PubMed Central

Khanna, Anjani

2012-01-01

A large number of glaucoma patients suffer from vision impairments that qualify as low vision. Additional difficulties associated with low vision include problems with glare, lighting, and contrast, which can make daily activities extremely challenging. This article elaborates on how low vision aids can help with various tasks that visually impaired glaucoma patients need to do each day, to take care of themselves and to lead an independent life. PMID:27990068

A Study of Dim Object Detection for the Space Surveillance Telescope

DTIC Science & Technology

2013-03-21

ENG-13-M-32 Abstract Current methods of dim object detection for space surveillance make use of a Gaussian log-likelihood-ratio-test-based...quantitatively comparing the efficacy of two methods for dim object detection , termed in this paper the point detector and the correlator, both of which rely... applications . It is used in national defense for detecting satellites. It is used to detecting space debris, which threatens both civilian and

The Effect on Vision of Light Scatter From HMD Visors and Aircraft Windscreens

DTIC Science & Technology

1999-01-01

This paper was cleared by ASC99-0447 on 5 Mar 1999 The Effect on Vision of Light Scatter from HMD Visors and Aircraft Windscreens Peter L. Marasco ...Medical Research Laboratory, Wright-Patterson AFB, Ohio. BIOGRAPHIES This paper was cleared by ASC99-0447 on 5 Mar 1999 Peter Marasco came to the U.S. Air...and improving optical test methods. Mr. Marasco received a BS degree from the University of Rochester in 1991 and an MS degree from the University of

ATTENUATION OF MULTI-TARGETED PROLIFERATION-LINKED SIGNALING BY 3,3′-DIINDOLYLMETHANE (DIM): FROM BENCH TO CLINIC

PubMed Central

Banerjee, Sanjeev; Kong, Dejuan; Wang, Zhiwei; Bao, Bin; Hillman, Gilda G; Sarkar, Fazlul H

2013-01-01

Emerging evidence provide credible support in favor of the potential role of bioactive products derived from ingesting cruciferous vegetables such as broccoli, brussel sprouts, cauliflower and cabbage. Among many compounds, 3,3′-Diindolylmethane (DIM) is generated in the acidic environment of the stomach following dimerization of Indole-3-Carbinol (I3C) monomers present in these classes of vegetables. Both I3C and DIM have been investigated for their use in preventing, inhibiting, and reversing the progression of cancer- as a chemopreventive agent. In this review, we summarize an updated, wide-ranging pleiotropic anti-tumor and biological effects elicited by DIM against tumor cells. It is unfeasible to point one single target as basis of cellular target of action of DIM. We emphasize key cellular and molecular events that are effectively modulated in the direction of inducing apoptosis and suppressing cell proliferation. Collectively, DIM orchestrates signaling through Ah receptor, NF-κB/Wnt/Akt/mTOR pathways impinging on cell cycle arrest, modulation of key cytochrome P450 enzymes, altering angiogenesis, invasion, metastasis and epigenetic behaviors of cancer cells. The ability of DIM to selectively induce tumor cells to undergo apoptosis has been observed in preclinical models, and thus it has been speculated in improving the therapeutic efficacy of other anticancer agents that have diverse molecular targets. Consequently, DIM has moved through preclinical development into phase-I clinical trials, thereby suggesting that DIM could be a promising and novel agent either alone or as an adjunct to conventional therapeutics such as chemo-radio therapy, and targeted therapies. An important development has been the availability of DIM formulation with superior bioavailability for humans. Therefore, DIM appears to be a promising chemopreventive agent or chemo-radio-sensitizer for the prevention of tumor recurrence and/or for the treatment of human malignancies. PMID

Single bright light exposure decreases sweet taste threshold in healthy volunteers.

PubMed

Srivastava, Shrikant; Donaldson, Lucy F; Rai, Dheeraj; Melichar, Jan K; Potokar, John

2013-10-01

Bright light exposure can alter circulating serotonin levels, and alteration of available serotonin by acute selective serotonin reuptake inhibition significantly lowers sweet but not salt taste recognition thresholds. We tested the hypothesis that bright light exposure would increase sweet but not salt taste sensitivity in healthy adults. Fourteen healthy volunteers were exposed to bright (10,000 lux) and dim (<20 lux) light for 30 min each, in counterbalanced order. Measures of taste perception (salt and sweet) and mood were determined at baseline, and before and after each light exposure period. Recognition thresholds for sucrose were significantly lower after bright but not dim light exposure. Thresholds for salt were unaffected by either condition. There were no significant changes in taste acuity, intensity or pleasantness for both the taste modalities and on visual analogue scales (VASs) for mood, anxiety, sleepiness and alertness, under either light condition. Brief bright light exposure reduces sweet but not salt taste recognition thresholds in healthy humans.

Biogenesis of light harvesting proteins.

PubMed

Dall'Osto, Luca; Bressan, Mauro; Bassi, Roberto

2015-09-01

The LHC family includes nuclear-encoded, integral thylakoid membrane proteins, most of which coordinate chlorophyll and xanthophyll chromophores. By assembling with the core complexes of both photosystems, LHCs form a flexible peripheral moiety for enhancing light-harvesting cross-section, regulating its efficiency and providing protection against photo-oxidative stress. Upon its first appearance, LHC proteins underwent evolutionary diversification into a large protein family with a complex genetic redundancy. Such differentiation appears as a crucial event in the adaptation of photosynthetic organisms to changing environmental conditions and land colonization. The structure of photosystems, including nuclear- and chloroplast-encoded subunits, presented the cell with a number of challenges for the control of the light harvesting function. Indeed, LHC-encoding messages are translated in the cytosol, and pre-proteins imported into the chloroplast, processed to their mature size and targeted to the thylakoids where are assembled with chromophores. Thus, a tight coordination between nuclear and plastid gene expression, in response to environmental stimuli, is required to adjust LHC composition during photoacclimation. In recent years, remarkable progress has been achieved in elucidating structure, function and regulatory pathways involving LHCs; however, a number of molecular details still await elucidation. In this review, we will provide an overview on the current knowledge on LHC biogenesis, ranging from organization of pigment-protein complexes to the modulation of gene expression, import and targeting to the photosynthetic membranes, and regulation of LHC assembly and turnover. Genes controlling these events are potential candidate for biotechnological applications aimed at optimizing light use efficiency of photosynthetic organisms. This article is part of a Special Issue entitled: Chloroplast biogenesis. Copyright © 2015 Elsevier B.V. All rights reserved.

Scotopic vision deficits in young monkeys exposed to lead.

PubMed

Bushnell, P J; Bowman, R E; Allen, J R; Marlar, R J

1977-04-15

Rhesus monkeys were reared on diets designed to produce blood lead concentrations of 14 (untreated), 55, or 85 micrograms per 100 milliliters for the first year of life. Eighteen months later, blood lead levels were normal in all animals. At this time, however, visual discrimination performance in the 85-microgram group was impaired under dim light relative both to their own performance under bright light and to the performance of the other groups under all light levels used. We interpret these results to reflect a deleterious, enduring impairment of scotopic visual function (night blindness) as a result of early lead intoxication.

Computer Vision-Based Structural Displacement Measurement Robust to Light-Induced Image Degradation for In-Service Bridges

PubMed Central

Lee, Junhwa; Lee, Kyoung-Chan; Cho, Soojin

2017-01-01

The displacement responses of a civil engineering structure can provide important information regarding structural behaviors that help in assessing safety and serviceability. A displacement measurement using conventional devices, such as the linear variable differential transformer (LVDT), is challenging owing to issues related to inconvenient sensor installation that often requires additional temporary structures. A promising alternative is offered by computer vision, which typically provides a low-cost and non-contact displacement measurement that converts the movement of an object, mostly an attached marker, in the captured images into structural displacement. However, there is limited research on addressing light-induced measurement error caused by the inevitable sunlight in field-testing conditions. This study presents a computer vision-based displacement measurement approach tailored to a field-testing environment with enhanced robustness to strong sunlight. An image-processing algorithm with an adaptive region-of-interest (ROI) is proposed to reliably determine a marker’s location even when the marker is indistinct due to unfavorable light. The performance of the proposed system is experimentally validated in both laboratory-scale and field experiments. PMID:29019950

Growth and Tuberization of Potato (Solanum tuberosum L.) under Continuous Light 1

PubMed Central

Wheeler, Raymond M.; Tibbitts, Theodore W.

1986-01-01

The growth and tuberization of potatoes (Solanum tuberosum L.) maintained for 6 weeks under four different regimes of continuous irradiance were compared to plants given 12 hours light and 12 hours dark. Treatments included: (a) continuous photosynthetic photon flux of 200 micromoles per square meter per second cool-white fluorescent (CWF); (b) continuous 400 micromoles per square meter per second CWF; (c) 12 hours 400 micromoles per square meter per second CWF plus 12 hours dim CWF at 5 micromoles per square meter per second; (d) 12 hours micromoles per square meter per second CWF plus 12 hours dim incandescent (INC) at 5 micromoles per square meter per second and a control treatment of 12 hours light at 400 micromoles per square meter per second CWF and 12 hours dark. The study included five cultivars ranging from early- to late-season types: `Norland,' `Superior,' `Norchip,' `Russet Burbank,' and `Kennebec.' Tuber development progressed well under continuous irradiation at 400 micromoles per square meter per second and under 12 hours irradiance and 12 hours dark, while tuber development was suppressed in all other light treatments. Continuous irradiation at 200 or 400 micromoles per square meter per second resulted in severe stunting and leaf malformation on `Superior' and `Kennebec' plants, but little or no injury and vigorous shoot growth in the other cultivars. No injury or stunting were apparent under 12-dim light or 12-dark treatments. Plants given 12 hours dim INC showed significantly greater stem elongation but less total biomass than plants in other treatments. The continuous light encouraged shoot growth over tuber growth but this trend was overridden by providing a high irradiance level. The variation among cultivars for tolerance to continuous lighting indicates that potato may be a useful species for photoinhibition studies. PMID:11539039

Mechanisms of the dimming and brightening aftereffects

PubMed Central

Bosten, Jenny M.; MacLeod, Donald I. A.

2013-01-01

Abstract Dimming and brightening aftereffects occur after exposure to a temporal luminance sawtooth stimulus: A subsequently presented steady test field appears to become progressively dimmer or brighter, depending on the polarity of the adapting sawtooth. Although described as “dimming” and “brightening,” it is plausible that a component of the aftereffects is based on contrast changes rather than on luminance changes. We conducted two experiments to reveal any contrast component. In the first we investigated whether the aftereffects result from the same mechanism that causes a polarity-selective loss in contrast sensitivity following luminance sawtooth adaptation. We manipulated test contrast: If a component of the aftereffect results from a polarity selective loss of contrast sensitivity we would expect that the aftereffects would differ in magnitude depending on the contrast polarity of the test fields. We found no effect of test-field polarity. In the second experiment we used an adapting sawtooth with a polarity consistent in contrast but alternating in luminance in order to induce a potential equivalent aftereffect of contrast. Again, we found no evidence that the aftereffects result from contrast adaptation. In a third experiment, we used S-cone isolating stimuli to discover whether there are S-cone dimming and brightening aftereffects. We found no aftereffects. However, in a fourth experiment we replicated Krauskopf and Zaidi's (1986) finding that adaptation to S-cone sawtooth stimuli affects thresholds for increment and decrement detection. The mechanism underlying the dimming and brightening aftereffects thus seems to be independent of the mechanism underlying the concurrent polarity selective reductions in contrast sensitivity. PMID:23695534

Regulation of Egr-1, VIP, and Shh mRNA and Egr-1 protein in the mouse retina by light and image quality.

PubMed

Brand, Christine; Burkhardt, Eva; Schaeffel, Frank; Choi, Jeong Won; Feldkaemper, Marita Pauline

2005-04-28

To analyze mRNA expression changes of Egr-1, VIP, and Shh under different light and treatment conditions in mice. The mRNA expression levels of the three genes and additionally the Egr-1 protein expression were compared in form deprived eyes and eyes with normal vision. Moreover, the influence of dark to light and light to dark transitions and of changes in retinal illumination on mRNA levels was investigated. Form deprivation of mice was induced by fitting frosted diffusers over one eye and an attentuation matched neutral density (ND) filter over the other eye. To measure the effects of retinal illumination changes on mRNA expression, animals were bilaterally fitted with different ND filters. Semiquantitative real-time RT-PCR was used to measure the mRNA levels and immunohistochemistry was applied to localize and detect Egr-1 protein. The expression levels of both Egr-1 mRNA and protein were reduced in form deprived eyes compared to their fellow eyes after 30 min and 1 h, respectively. Egr-1 mRNA was strikingly upregulated both after dark to light and light to dark transitions, whereas minor changes in retinal illumination by covering the eyes with neutral density filters did not alter Egr-1 mRNA expression. In mice, the mRNA levels of VIP and Shh were not affected by form deprivation, but they were found to be regulated depending on the time of day. Both Egr-1 mRNA and protein expression levels were strongly regulated by light, especially by transitions between light and darkness. Image contrast may exert an additional influence on mRNA and protein expression of Egr-1, particularly in the cells in the ganglion cell layer and in bipolar cells.

Comparison of vision through surface modulated and spatial light modulated multifocal optics.

PubMed

Vinas, Maria; Dorronsoro, Carlos; Radhakrishnan, Aiswaryah; Benedi-Garcia, Clara; LaVilla, Edward Anthony; Schwiegerling, Jim; Marcos, Susana

2017-04-01

Spatial-light-modulators (SLM) are increasingly used as active elements in adaptive optics (AO) systems to simulate optical corrections, in particular multifocal presbyopic corrections. In this study, we compared vision with lathe-manufactured multi-zone (2-4) multifocal, angularly and radially, segmented surfaces and through the same corrections simulated with a SLM in a custom-developed two-active-element AO visual simulator. We found that perceived visual quality measured through real manufactured surfaces and SLM-simulated phase maps corresponded highly. Optical simulations predicted differences in perceived visual quality across different designs at Far distance, but showed some discrepancies at intermediate and near.

Coping with copepods: do right whales (Eubalaena glacialis) forage visually in dark waters?

PubMed

Cronin, Thomas W; Fasick, Jeffry I; Schweikert, Lorian E; Johnsen, Sönke; Kezmoh, Lorren J; Baumgartner, Mark F

2017-04-05

North Atlantic right whales ( Eubalaena glacialis ) feed during the spring and early summer in marine waters off the northeast coast of North America. Their food primarily consists of planktonic copepods, Calanus finmarchicus , which they consume in large numbers by ram filter feeding. The coastal waters where these whales forage are turbid, but they successfully locate copepod swarms during the day at depths exceeding 100 m, where light is very dim and copepod patches may be difficult to see. Using models of E. glacialis visual sensitivity together with measurements of light in waters near Cape Cod where they feed and of light attenuation by living copepods in seawater, we evaluated the potential for visual foraging by these whales. Our results suggest that vision may be useful for finding copepod patches, particularly if E. glacialis searches overhead for silhouetted masses or layers of copepods. This should permit the whales to locate C. finmarchicus visually throughout most daylight hours at depths throughout their foraging range. Looking laterally, the whales might also be able to see copepod patches at short range near the surface.This article is part of the themed issue 'Vision in dim light'. © 2017 The Author(s).

Explicit spatiotemporal simulation of receptor-G protein coupling in rod cell disk membranes.

PubMed

Schöneberg, Johannes; Heck, Martin; Hofmann, Klaus Peter; Noé, Frank

2014-09-02

Dim-light vision is mediated by retinal rod cells. Rhodopsin (R), a G-protein-coupled receptor, switches to its active form (R(∗)) in response to absorbing a single photon and activates multiple copies of the G-protein transducin (G) that trigger further downstream reactions of the phototransduction cascade. The classical assumption is that R and G are uniformly distributed and freely diffusing on disk membranes. Recent experimental findings have challenged this view by showing specific R architectures, including RG precomplexes, nonuniform R density, specific R arrangements, and immobile fractions of R. Here, we derive a physical model that describes the first steps of the photoactivation cascade in spatiotemporal detail and single-molecule resolution. The model was implemented in the ReaDDy software for particle-based reaction-diffusion simulations. Detailed kinetic in vitro experiments are used to parametrize the reaction rates and diffusion constants of R and G. Particle diffusion and G activation are then studied under different conditions of R-R interaction. It is found that the classical free-diffusion model is consistent with the available kinetic data. The existence of precomplexes between inactive R and G is only consistent with the data if these precomplexes are weak, with much larger dissociation rates than suggested elsewhere. Microarchitectures of R, such as dimer racks, would effectively immobilize R but have little impact on the diffusivity of G and on the overall amplification of the cascade at the level of the G protein. Copyright © 2014 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Microscopic vision modeling method by direct mapping analysis for micro-gripping system with stereo light microscope.

PubMed

Wang, Yuezong; Zhao, Zhizhong; Wang, Junshuai

2016-04-01

We present a novel and high-precision microscopic vision modeling method, which can be used for 3D data reconstruction in micro-gripping system with stereo light microscope. This method consists of four parts: image distortion correction, disparity distortion correction, initial vision model and residual compensation model. First, the method of image distortion correction is proposed. Image data required by image distortion correction comes from stereo images of calibration sample. The geometric features of image distortions can be predicted though the shape deformation of lines constructed by grid points in stereo images. Linear and polynomial fitting methods are applied to correct image distortions. Second, shape deformation features of disparity distribution are discussed. The method of disparity distortion correction is proposed. Polynomial fitting method is applied to correct disparity distortion. Third, a microscopic vision model is derived, which consists of two models, i.e., initial vision model and residual compensation model. We derive initial vision model by the analysis of direct mapping relationship between object and image points. Residual compensation model is derived based on the residual analysis of initial vision model. The results show that with maximum reconstruction distance of 4.1mm in X direction, 2.9mm in Y direction and 2.25mm in Z direction, our model achieves a precision of 0.01mm in X and Y directions and 0.015mm in Z direction. Comparison of our model with traditional pinhole camera model shows that two kinds of models have a similar reconstruction precision of X coordinates. However, traditional pinhole camera model has a lower precision of Y and Z coordinates than our model. The method proposed in this paper is very helpful for the micro-gripping system based on SLM microscopic vision. Copyright © 2016 Elsevier Ltd. All rights reserved.

The evolution of vision.

PubMed

Gehring, Walter J

2014-01-01

In this review, the evolution of vision is retraced from its putative origins in cyanobacteria to humans. Circadian oscillatory clocks, phototropism, and phototaxis require the capability to detect light. Photosensory proteins allow us to reconstruct molecular phylogenetic trees. The evolution of animal eyes leading from an ancestral prototype to highly complex image forming eyes can be deciphered on the basis of evolutionary developmental genetic experiments and comparative genomics. As all bilaterian animals share the same master control gene, Pax6, and the same retinal and pigment cell determination genes, we conclude that the different eye-types originated monophyletically and subsequently diversified by divergent, parallel, or convergent evolution. © 2012 Wiley Periodicals, Inc.

Effects of lighting illuminance levels on stair negotiation performance in individuals with visual impairment.

PubMed

Shaheen, Aliah F; Sourlas, Alexandros; Horton, Khim; McLean, Christopher; Ewins, David; Gould, David; Ghoussayni, Salim

2018-04-01

Stair-related falls of older people cause a substantial financial and social burden. Deterioration of the visual system amongst other factors put older people at a high risk of falling. Improved lighting is often recommended. The aim of this study was to investigate the effect of lighting illuminance on stair negotiation performance in older individuals with visual impairment. Eleven participants aged 60 or over with a vision of 6/18 or worse ascended and descended a staircase under: 50 lx, 100 lx, 200 lx, 300 lx and distributed 200 lx lighting. A motion capture system was used to measure movements of the lower limb. Clearance, clearance variability, temporal and spatial parameters and joint/segment kinematics were computed. There was no effect on clearance or clearance variability. Participants had lower speed, cadence, increased cycle time and stance time in the 50 lx compared to 300 lx and distributed 200 lx lighting in descent. The minimum hip angle in ascent was increased in the 200 lx lighting. Clearance was found to be moderately correlated with balance scores. Individuals with visual impairment adopt precautionary gait in dim lighting conditions. This does not always result in improvements in the parameters associated with risk of falling (e.g. clearance). Copyright © 2018 Elsevier Ltd. All rights reserved.

The impact on vision of aspheric to spherical monofocal intraocular lenses in cataract surgery: a systematic review with meta-analysis.

PubMed

Schuster, Alexander K; Tesarz, Jonas; Vossmerbaeumer, Urs

2013-11-01

To provide a summary of the impact on vision of an aspheric intraocular lens (IOL) compared with a spherical IOL in cataract surgery. Systematic review with meta-analysis. Patients from published randomized controlled trials (RCTs) of cataract surgery with aspheric compared with spherical monofocal IOL implantation. We systematically searched the peer-reviewed literature in MEDLINE, EMBASE, Web of Science, BIOSIS, and the Cochrane Library according to the Cochrane Collaboration method to identify relevant RCTs. The inclusion criteria were RCTs on cataract surgery comparing the use of aspheric versus spherical IOL implantation that assessed visual acuity, contrast sensitivity, or quality of vision. The effects were calculated as mean differences or standardized mean differences (Hedges' g) and pooled using random-effect models. Best-corrected visual acuity (BCVA), contrast sensitivity, and subjective perception of the quality of vision. Forty-three studies provided data and were included, comprising 2076 eyes implanted with aspheric IOLs and 2034 eyes implanted with spherical IOLs. The BCVA showed a significant difference for aspheric IOLs (-0.01 logarithm of the minimum angle of resolution; 95% confidence interval [CI], -0.02 to -0.00). For contrast sensitivity, a significant advantage for aspheric IOLs was found under photopic and mesopic light conditions (photopic: Hedges' g 0.42, 95% CI 0.24-0.61 (3 cycles per degree [cpd]) to 0.53, 95% CI 0.33-0.73 (12 cpd); mesopic: Hedges' g 0.49, 95% CI 0.23-0.75 (1.5 cpd) to 0.76, 95% CI 0.52-1.00 (18 cpd)). Questionnaires targeting the subjective perception of quality of vision yielded less conclusive results. Overall, a patient may achieve better contrast sensitivity with an aspheric IOL than with a spherical IOL, especially under dim light. There was no clinically relevant difference in BCVA between aspheric and spherical IOL implantation. The findings on the subjective perception of visual quality were heterogeneous

Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins

PubMed Central

Polstein, Lauren R.; Gersbach, Charles A.

2014-01-01

The coupling of light-inducible protein-protein interactions with gene regulation systems has enabled the control of gene expression with light. In particular, heterodimer protein pairs from plants can be used to engineer a gene regulation system in mammalian cells that is reversible, repeatable, tunable, controllable in a spatiotemporal manner, and targetable to any DNA sequence. This system, Light-Inducible Transcription using Engineered Zinc finger proteins (LITEZ), is based on the blue light-induced interaction of GIGANTEA and the LOV domain of FKF1 that drives the localization of a transcriptional activator to the DNA-binding site of a highly customizable engineered zinc finger protein. This chapter provides methods for modifying LITEZ to target new DNA sequences, engineering a programmable LED array to illuminate cell cultures, and using the modified LITEZ system to achieve spatiotemporal control of transgene expression in mammalian cells. PMID:24718797

Computer vision syndrome: a review.

PubMed

Blehm, Clayton; Vishnu, Seema; Khattak, Ashbala; Mitra, Shrabanee; Yee, Richard W

2005-01-01

As computers become part of our everyday life, more and more people are experiencing a variety of ocular symptoms related to computer use. These include eyestrain, tired eyes, irritation, redness, blurred vision, and double vision, collectively referred to as computer vision syndrome. This article describes both the characteristics and treatment modalities that are available at this time. Computer vision syndrome symptoms may be the cause of ocular (ocular-surface abnormalities or accommodative spasms) and/or extraocular (ergonomic) etiologies. However, the major contributor to computer vision syndrome symptoms by far appears to be dry eye. The visual effects of various display characteristics such as lighting, glare, display quality, refresh rates, and radiation are also discussed. Treatment requires a multidirectional approach combining ocular therapy with adjustment of the workstation. Proper lighting, anti-glare filters, ergonomic positioning of computer monitor and regular work breaks may help improve visual comfort. Lubricating eye drops and special computer glasses help relieve ocular surface-related symptoms. More work needs to be done to specifically define the processes that cause computer vision syndrome and to develop and improve effective treatments that successfully address these causes.

Advanced helmet vision system (AHVS) integrated night vision helmet mounted display (HMD)

NASA Astrophysics Data System (ADS)

Ashcraft, Todd W.; Atac, Robert

2012-06-01

Gentex Corporation, under contract to Naval Air Systems Command (AIR 4.0T), designed the Advanced Helmet Vision System to provide aircrew with 24-hour, visor-projected binocular night vision and HMD capability. AHVS integrates numerous key technologies, including high brightness Light Emitting Diode (LED)-based digital light engines, advanced lightweight optical materials and manufacturing processes, and innovations in graphics processing software. This paper reviews the current status of miniaturization and integration with the latest two-part Gentex modular helmet, highlights the lessons learned from previous AHVS phases, and discusses plans for qualification and flight testing.

Selections from 2016: A Connection Between Solar Explosions and Dimming on the Sun

NASA Astrophysics Data System (ADS)

Kohler, Susanna

2016-12-01

Editors note:In these last two weeks of 2016, well be looking at a few selections that we havent yet discussed on AAS Nova from among the most-downloaded paperspublished in AAS journals this year. The usual posting schedule will resume after the AAS winter meeting.The Nature of CME-Flare-Associated Coronal DimmingPublished June2016Main takeaway:The Solar Dynamics Observatory (SDO) observed a large solar eruption at the end of December 2011. Scientists Jianxia Cheng (Shanghai Astronomical Observatory and the Chinese Academy of Sciences) and Jiong Qiu (Montana State University) studied this coronal mass ejection and the associated flaring on the Suns surface. They found that this activity was accompanied by dimming in the Suns corona near the ends of the flare ribbons.Why its interesting:The process of coronal dimming isnt fully understood, but Cheng and Qius observations provide a clear link between coronal dimming and eruptions of plasma and energy from the Sun. The locations of the dimming the footpoints of the two flare ribbons and the timing relative to the eruption suggests that coronal dimming is caused by the ejection of hot plasma from the Suns surface.How this process was studied:There are a number of satellites dedicated to observing the Sun, and several of them were used to study this explosion. Data from SDOs Atmospheric Imaging Assembly (which images in extreme ultraviolet) and its Helioseismic and Magnetic Imager (which measures magnetic fields) were used as well as observations from STEREO, the pair of satellites orbiting the Sun at 90 from SDO.CitationJ. X. Cheng and J. Qiu 2016 ApJ 825 37. doi:10.3847/0004-637X/825/1/37

Degas: Vision and Perception.

ERIC Educational Resources Information Center

Kendall, Richard

1988-01-01

The art of Edgar Degas is discussed in relation to his impaired vision, including amblyopia, later blindness in one eye, corneal scarring, and photophobia. Examined are ways in which Degas compensated for vision problems, and dominant themes of his art such as the process of perception and spots of brilliant light. (Author/JDD)

B-DIM impairs radiation-induced survival pathways independently of androgen receptor expression and augments radiation efficacy in prostate cancer.

PubMed

Singh-Gupta, Vinita; Banerjee, Sanjeev; Yunker, Christopher K; Rakowski, Joseph T; Joiner, Michael C; Konski, Andre A; Sarkar, Fazlul H; Hillman, Gilda G

2012-05-01

Increased consumption of cruciferous vegetables is associated with decreased risk in prostate cancer (PCa). The active compound in cruciferous vegetables appears to be the self dimerized product [3,3'-diindolylmethane (DIM)] of indole-3-carbinol (I3C). Nutritional grade B-DIM (absorption-enhanced) has proven safe in a Phase I trial in PCa. We investigated the anti-cancer activity of B-DIM as a new biological approach to improve the effects of radiotherapy for hormone refractory prostate cancer cells, which were either positive or negative for androgen receptor (AR) expression. B-DIM inhibited cell growth in a dose-dependent manner in both PC-3 (AR-) and C4-2B (AR+) cell lines. B-DIM was effective at increasing radiation-induced cell killing in both cell lines, independently of AR expression. B-DIM inhibited NF-κB and HIF-1α DNA activities and blocked radiation-induced activation of these transcription factors in both PC-3 and C4-2B cells. In C4-2B (AR+) cells, AR expression and nuclear localization were significantly increased by radiation. However, B-DIM abrogated the radiation-induced AR increased expression and trafficking to the nucleus, which was consistent with decreased PSA secretion. In vivo, treatment of PC-3 prostate tumors in nude mice with B-DIM and radiation resulted in significant primary tumor growth inhibition and control of metastasis to para-aortic lymph nodes. These studies demonstrate that B-DIM augments radiation-induced cell killing and tumor growth inhibition. B-DIM impairs critical survival signaling pathways activated by radiation, leading to enhanced cell killing. These novel observations suggest that B-DIM could be used as a safe compound to enhance the efficacy of radiotherapy for castrate-resistant PCa. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Stem/progenitor cell-like properties of desmoglein 3dim cells in primary and immortalized keratinocyte lines.

PubMed

Wan, Hong; Yuan, Ming; Simpson, Cathy; Allen, Kirsty; Gavins, Felicity N E; Ikram, Mohammed S; Basu, Subham; Baksh, Nuzhat; O'Toole, Edel A; Hart, Ian R

2007-05-01

We showed previously that primary keratinocytes selected for low desmoglein 3 (Dsg3) expression levels exhibited increased colony-forming efficiency and heightened proliferative potential relative to cells with higher Dsg3 expression levels, characteristics consistent with a more "stem/progenitor cell-like" phenotype. Here, we have confirmed that Dsg3(dim) cells derived from cultured primary human adult keratinocytes have comparability with alpha(6)(bri)/CD71(dim) stem cells in terms of colony-forming efficiency. Moreover, these Dsg3(dim) cells exhibit increased reconstituting ability in in vitro organotypic culture on de-epidermalized dermis (DED); they are small, actively cycling cells, and they express elevated levels of various p63 isoforms. In parallel, using the two immortalized keratinocyte cell lines HaCaT and NTERT, we obtained essentially similar though occasionally different findings. Thus, reduced colony-forming efficiency by Dsg3(bri) cells consistently was observed in both cell lines even though the cell cycle profile and levels of p63 isoforms in the bri and dim populations differed between these two cell lines. Dsg3(dim) cells from both immortalized lines produced thicker and better ordered hierarchical structural organization of reconstituted epidermis relative to Dsg3(bri) and sorted control cells. Dsg3(dim) HaCaT cells also show sebocyte-like differentiation in the basal compartment of skin reconstituted after a 4-week organotypic culture. No differences in percentages of side population cells (also a putative marker of stem cells) were detected between Dsg3(dim) and Dsg3(bri) populations. Taken together our data indicate that Dsg3(dim) populations from primary human adult keratinocytes and long-term established keratinocyte lines possess certain stem/progenitor cell-like properties, although the side population characteristic is not one of these features. Disclosure of potential conflicts of interest is found at the end of this article.

Neuronal mechanisms underlying differences in spatial resolution between darks and lights in human vision.

PubMed

Pons, Carmen; Mazade, Reece; Jin, Jianzhong; Dul, Mitchell W; Zaidi, Qasim; Alonso, Jose-Manuel

2017-12-01

Artists and astronomers noticed centuries ago that humans perceive dark features in an image differently from light ones; however, the neuronal mechanisms underlying these dark/light asymmetries remained unknown. Based on computational modeling of neuronal responses, we have previously proposed that such perceptual dark/light asymmetries originate from a luminance/response saturation within the ON retinal pathway. Consistent with this prediction, here we show that stimulus conditions that increase ON luminance/response saturation (e.g., dark backgrounds) or its effect on light stimuli (e.g., optical blur) impair the perceptual discrimination and salience of light targets more than dark targets in human vision. We also show that, in cat visual cortex, the magnitude of the ON luminance/response saturation remains relatively constant under a wide range of luminance conditions that are common indoors, and only shifts away from the lowest luminance contrasts under low mesopic light. Finally, we show that the ON luminance/response saturation affects visual salience mostly when the high spatial frequencies of the image are reduced by poor illumination or optical blur. Because both low luminance and optical blur are risk factors in myopia, our results suggest a possible neuronal mechanism linking myopia progression with the function of the ON visual pathway.

Neuronal mechanisms underlying differences in spatial resolution between darks and lights in human vision

PubMed Central

Pons, Carmen; Mazade, Reece; Jin, Jianzhong; Dul, Mitchell W.; Zaidi, Qasim; Alonso, Jose-Manuel

2017-01-01

Artists and astronomers noticed centuries ago that humans perceive dark features in an image differently from light ones; however, the neuronal mechanisms underlying these dark/light asymmetries remained unknown. Based on computational modeling of neuronal responses, we have previously proposed that such perceptual dark/light asymmetries originate from a luminance/response saturation within the ON retinal pathway. Consistent with this prediction, here we show that stimulus conditions that increase ON luminance/response saturation (e.g., dark backgrounds) or its effect on light stimuli (e.g., optical blur) impair the perceptual discrimination and salience of light targets more than dark targets in human vision. We also show that, in cat visual cortex, the magnitude of the ON luminance/response saturation remains relatively constant under a wide range of luminance conditions that are common indoors, and only shifts away from the lowest luminance contrasts under low mesopic light. Finally, we show that the ON luminance/response saturation affects visual salience mostly when the high spatial frequencies of the image are reduced by poor illumination or optical blur. Because both low luminance and optical blur are risk factors in myopia, our results suggest a possible neuronal mechanism linking myopia progression with the function of the ON visual pathway. PMID:29196762

Comparison of vision through surface modulated and spatial light modulated multifocal optics

PubMed Central

Vinas, Maria; Dorronsoro, Carlos; Radhakrishnan, Aiswaryah; Benedi-Garcia, Clara; LaVilla, Edward Anthony; Schwiegerling, Jim; Marcos, Susana

2017-01-01

Spatial-light-modulators (SLM) are increasingly used as active elements in adaptive optics (AO) systems to simulate optical corrections, in particular multifocal presbyopic corrections. In this study, we compared vision with lathe-manufactured multi-zone (2-4) multifocal, angularly and radially, segmented surfaces and through the same corrections simulated with a SLM in a custom-developed two-active-element AO visual simulator. We found that perceived visual quality measured through real manufactured surfaces and SLM-simulated phase maps corresponded highly. Optical simulations predicted differences in perceived visual quality across different designs at Far distance, but showed some discrepancies at intermediate and near. PMID:28736655

Light Battalion Task Force Reconnaissance and Surveillance: Clear Vision or Groping in the Dark

DTIC Science & Technology

1990-12-23

1 gP.Oi. t@PO n b fl lot this colita n of information s etimated to a.’vae I hout car reions. including " tme for arviewing r r ct ,O matc ng eaiting...SUBTITLE S . FUNDING NUMBERS LIGHT BATTALION TASK FORCE RECONNAISSANCE AND SURVEILLANCE: CLEAR VISION OR GROPING IN TIHE DARK (U) 6. AUTHOR( S ) XAJ...DAVID B. LACQUEMENT 7. PERFORMING ORGANIZATION NAME( S ) AND ADDRESS(ES) B. PERFORMING ORGANIZATION SCHOOL OF ADVANCED MILITARY STUDIES REPORT NUMBER ATTN

Restoration of Vision in the pde6β-deficient Dog, a Large Animal Model of Rod-cone Dystrophy

PubMed Central

Petit, Lolita; Lhériteau, Elsa; Weber, Michel; Le Meur, Guylène; Deschamps, Jack-Yves; Provost, Nathalie; Mendes-Madeira, Alexandra; Libeau, Lyse; Guihal, Caroline; Colle, Marie-Anne; Moullier, Philippe; Rolling, Fabienne

2012-01-01

Defects in the β subunit of rod cGMP phosphodiesterase 6 (PDE6β) are associated with autosomal recessive retinitis pigmentosa (RP), a childhood blinding disease with early retinal degeneration and vision loss. To date, there is no treatment for this pathology. The aim of this preclinical study was to test recombinant adeno-associated virus (AAV)-mediated gene addition therapy in the rod-cone dysplasia type 1 (rcd1) dog, a large animal model of naturally occurring PDE6β deficiency that strongly resembles the human pathology. A total of eight rcd1 dogs were injected subretinally with AAV2/5RK.cpde6β (n = 4) or AAV2/8RK.cpde6β (n = 4). In vivo and post-mortem morphological analysis showed a significant preservation of the retinal structure in transduced areas of both AAV2/5RK.cpde6β- and AAV2/8RK.cpde6β-treated retinas. Moreover, substantial rod-derived electroretinography (ERG) signals were recorded as soon as 1 month postinjection (35% of normal eyes) and remained stable for at least 18 months (the duration of the study) in treated eyes. Rod-responses were undetectable in untreated contralateral eyes. Most importantly, dim-light vision was restored in all treated rcd1 dogs. These results demonstrate for the first time that gene therapy effectively restores long-term retinal function and vision in a large animal model of autosomal recessive rod-cone dystrophy, and provide great promise for human treatment. PMID:22828504

Effects of solar dimming and brightening on the terrestrial carbon sink

NASA Astrophysics Data System (ADS)

Mercado, L.; Bellouin, N.; Sitch, S.; Boucher, O.; Huntingford, C.; Wild, M.; Cox, P. M.

2009-04-01

Plant photosynthesis increases with solar radiation. Recent studies have shown that photosynthesis is more efficient under diffuse light conditions (Gu et al., 2003, Niyogi et al., 2004, Oliveira et al., 2007, Roderick et al., 2001). Changes in cloud cover and atmospheric aerosol loadings from either volcanic and anthropogenic sources, modify the total radiation reaching the surface and the fraction of this radiation which is diffuse, with uncertain overall effects on plant productivity and the global land carbon sink. A decrease in total solar radiation (Liepert, 2002, Stanhill and Cohen, 2001, Wild et al., 2005) has been observed at the Earth surface over the 1950-1990 period, called solar dimming. Such dimming gradually started to transform into brightening in some regions of the world since the late 1980s (Wild et al. 2005). The effect of these changes in total solar radiation and associated changes in diffuse radiation and diffuse fraction on the land biosphere has not yet been accounted for in global carbon cycle simulations because such models lack the mechanism that includes the diffuse irradiance effects on photosynthesis In this study we estimate the total impact of variations in clouds and atmospheric aerosols on the land carbon sink using a global land carbon cycle model modified to account for the effects of variations in both direct and diffuse radiation on canopy photosynthesis (Mercado et al., 2007) during the global dimming and brightening period. References Gu L.H., Baldocchi D.D., Wofsy S.C., Munger J.W., Michalsky J.J., Urbanski S.P. & Boden T.A. (2003) Response of a deciduous forest to the Mount Pinatubo eruption: Enhanced photosynthesis. Science, 299, 2035-2038. Liepert B.G. (2002) Observed reductions of surface solar radiation at sites in the United States and worldwide from 1961 to 1990. 29, 1421. Mercado L.M., Huntingford C., Gash J.H.C., Cox P.M. & Jogireddy V. (2007) Improving the representation of radiation interception and

Combined effects of exposure to dim light at night and fine particulate matter on C3H/HeNHsd mice.

PubMed

Hogan, Matthew K; Kovalycsik, Taylor; Sun, Qinghua; Rajagopalan, Sanjay; Nelson, Randy J

2015-11-01

Air and light pollution contribute to fetal abnormalities, increase prevalence of cancer, metabolic and cardiorespiratory diseases, and central nervous system (CNS) disorders. A component of air pollution, particulate matter, and the phenomenon of dim light at night (dLAN) both result in neuroinflammation, which has been implicated in several CNS disorders. The combinatorial role of these pollutants on health outcomes has not been assessed. Male C3H/HeNHsd mice, with intact melatonin production, were used to model humans exposed to circadian disruption by dLAN and contaminated environmental air. We hypothesized exposure to 2.5 μm of particulate matter (PM2.5) and dLAN (5lx) combines to upregulate neuroinflammatory cytokine expression and alter hippocampal morphology compared to mice exposed to filtered air (FA) and housed under dark nights (LD). We also hypothesized that exposure to PM2.5 and dLAN provokes anxiety-like and depressive-like responses. For four weeks, four groups of mice were simultaneously exposed to ambient concentrated PM2.5 or FA and/or dLAN or LD. Following exposure, mice underwent several behavioral assays and hippocampi were collected for qPCR and morphological analyses. Our results are generally comparable to previous PM2.5 and dLAN reports conducted on mice and implicate PM2.5 and dLAN as potential factors contributing to depression and anxiety. Short-term exposure to PM2.5 and dLAN upregulated neuroinflammatory cytokines and altered CA1 hippocampal structural changes, as well as provoked depressive-like responses (anhedonia). However, combined, PM2.5 and dLAN exposure did not have additive effects, as hypothesized, suggesting a ceiling effect of neuroinflammation may exist in response to multiple pollutants. Copyright © 2015 Elsevier B.V. All rights reserved.

Dim light melatonin onset in alcohol-dependent men and women compared with healthy controls.

PubMed

Conroy, Deirdre A; Hairston, Ilana S; Arnedt, J Todd; Hoffmann, Robert F; Armitage, Roseanne; Brower, Kirk J

2012-02-01

Sleep disturbances in alcohol-dependent (AD) individuals may persist despite abstinence from alcohol and can influence the course of the disorder. Although the mechanisms of sleep disturbances of AD are not well understood and some evidence suggests dysregulation of circadian rhythms, dim light melatonin onset (DLMO) has not previously been assessed in AD versus healthy control (HC) individuals in a sample that varied by sex and race. The authors assessed 52 AD participants (mean ± SD age: 36.0 ± 11.0 yrs of age, 10 women) who were 3-12 wks since their last drink (abstinence: 57.9 ± 19.3 d) and 19 age- and sex-matched HCs (34.4 ± 10.6 yrs, 5 women). Following a 23:00-06:00 h at-home sleep schedule for at least 5 d and screening/baseline nights in the sleep laboratory, participants underwent a 3-h extension of wakefulness (02:00 h bedtime) during which salivary melatonin samples were collected every 30 min beginning at 19:30 h. The time of DLMO was the primary measure of circadian physiology and was assessed with two commonly used methodologies. There was a slower rate of rise and lower maximal amplitude of the melatonin rhythm in the AD group. DLMO varied by the method used to derive it. Using 3 pg/mL as threshold, no significant differences were found between the AD and HC groups. Using 2 standard deviations above the mean of the first three samples, the DLMO in AD occurred significantly later, 21:02 ± 00:41 h, than in HC, 20:44 ± 00:21 h (t = -2.4, p = .02). Although melatonin in the AD group appears to have a slower rate of rise, using well-established criteria to assess the salivary DLMO did not reveal differences between AD and HC participants. Only when capturing melatonin when it is already rising was DLMO found to be significantly delayed by a mean 18 min in AD participants. Future circadian analyses on alcoholics should account for these methodological caveats.

Hybrid daylight/light-emitting diode illumination system for indoor lighting.

PubMed

Ge, Aiming; Qiu, Peng; Cai, Jinlin; Wang, Wei; Wang, Junwei

2014-03-20

A hybrid illumination method using both daylight and light-emitting diodes (LEDs) for indoor lighting is presented in this study. The daylight can be introduced into the indoor space by a panel-integration system. The daylight part and LEDs are combined within a specific luminaire that can provide uniform illumination. The LEDs can be turned on and dimmed through closed-loop control when the daylight illuminance is inadequate. We simulated the illumination and calculated the indoor lighting efficiency of our hybrid daylight and LED lighting system, and compared this with that of LED and fluorescent lighting systems. Simulation results show that the efficiency of the hybrid daylight/LED illumination method is better than that of LED and traditional lighting systems, under the same lighting conditions and lighting time; the method has hybrid lighting average energy savings of T5 66.28%, and that of the LEDs is 41.62%.

Performance Characterization of Dye-Sensitized Photovoltaics under Indoor Lighting.

PubMed

Chen, Chia-Yuan; Jian, Zih-Hong; Huang, Shih-Han; Lee, Kun-Mu; Kao, Ming-Hsuan; Shen, Chang-Hong; Shieh, Jia-Min; Wang, Chin-Li; Chang, Chiung-Wen; Lin, Bo-Zhi; Lin, Ching-Yao; Chang, Ting-Kuang; Chi, Yun; Chi, Cheng-Yu; Wang, Wei-Ting; Tai, Yian; Lu, Ming-De; Tung, Yung-Liang; Chou, Po-Ting; Wu, Wen-Ti; Chow, Tahsin J; Chen, Peter; Luo, Xiang-Hao; Lee, Yuh-Lang; Wu, Chih-Chung; Chen, Chih-Ming; Yeh, Chen-Yu; Fan, Miao-Syuan; Peng, Jia-De; Ho, Kuo-Chuan; Liu, Yu-Nan; Lee, Hsiao-Yi; Chen, Chien-Yu; Lin, Hao-Wu; Yen, Chia-Te; Huang, Yu-Ching; Tsao, Cheng-Si; Ting, Yu-Chien; Wei, Tzu-Chien; Wu, Chun-Guey

2017-04-20

Indoor utilization of emerging photovoltaics is promising; however, efficiency characterization under room lighting is challenging. We report the first round-robin interlaboratory study of performance measurement for dye-sensitized photovoltaics (cells and mini-modules) and one silicon solar cell under a fluorescent dim light. Among 15 research groups, the relative deviation in power conversion efficiency (PCE) of the samples reaches an unprecedented 152%. On the basis of the comprehensive results, the gap between photometry and radiometry measurements and the response of devices to the dim illumination are identified as critical obstacles to the correct PCE. Therefore, we use an illuminometer as a prime standard with a spectroradiometer to quantify the intensity of indoor lighting and adopt the reverse-biased current-voltage (I-V) characteristics as an indicator to qualify the I-V sampling time for dye-sensitized photovoltaics. The recommendations can brighten the prospects of emerging photovoltaics for indoor applications.

Pulsed-light imaging for fluorescence guided surgery under normal room lighting.

PubMed

Sexton, Kristian; Davis, Scott C; McClatchy, David; Valdes, Pablo A; Kanick, Stephen C; Paulsen, Keith D; Roberts, David W; Pogue, Brian W

2013-09-01

Fluorescence guided surgery (FGS) is an emerging technology that has demonstrated improved surgical outcomes. However, dim lighting conditions required by current FGS systems are disruptive to standard surgical workflow. We present a novel FGS system capable of imaging fluorescence under normal room light by using pulsed excitation and gated acquisition. Images from tissue-simulating phantoms confirm visual detection down to 0.25 μM of protoporphyrin IX under 125 μW/cm2 of ambient light, more than an order of magnitude lower than that measured with the Zeiss Pentero in the dark. Resection of orthotopic brain tumors in mice also suggests that the pulsed-light system provides superior sensitivity in vivo.

Design of Light-Controlled Protein Conformations and Functions.

PubMed

Ritterson, Ryan S; Hoersch, Daniel; Barlow, Kyle A; Kortemme, Tanja

2016-01-01

In recent years, interest in controlling protein function with light has increased. Light offers a number of unique advantages over other methods, including spatial and temporal control and high selectivity. Here, we describe a general protocol for engineering a protein to be controllable with light via reaction with an exogenously introduced photoisomerizable small molecule and illustrate our protocol with two examples from the literature: the engineering of the calcium affinity of the cell-cell adhesion protein cadherin, which is an example of a protein that switches from a native to a disrupted state (Ritterson et al. J Am Chem Soc (2013) 135:12516-12519), and the engineering of the opening and closing of the chaperonin Mm-cpn, an example of a switch between two functional states (Hoersch et al.: Nat Nanotechn (2013) 8:928-932). This protocol guides the user from considering which proteins may be most amenable to this type of engineering, to considerations of how and where to make the desired changes, to the assays required to test for functionality.

Low vision in east African blind school students: need for optical low vision services.

PubMed

Silver, J; Gilbert, C E; Spoerer, P; Foster, A

1995-09-01

There is increasing awareness of the needs of children with low vision, particularly in developing countries where programmes of integrated education are being developed. However, appropriate low vision services are usually not available or affordable. The aims of this study were, firstly, to assess the need for spectacles and optical low vision devices in students with low vision in schools for the blind in Kenya and Uganda; secondly, to evaluate inexpensive locally produced low vision devices; and, finally, to evaluate simple methods of identifying those low vision students who could read N5 to N8 print after low vision assessment. A total of 230 students were examined (51 school and 16 university students in Uganda and 163 students in Kenya, aged 5-22 years), 147 of whom had a visual acuity of less than 6/18 to perception of light in the better eye at presentation. After refraction seven of the 147 achieved 6/18 or better. Eighty two (58.6%) of the 140 students with low vision (corrected visual acuity in the better eye of less than 6/18 to light perception) had refractive errors of more than 2 dioptres in the better eye, and 38 (27.1%) had more than 2 dioptres of astigmatism. Forty six per cent of students with low vision (n = 64) could read N5-N8 print unaided or with spectacles, as could a further 33% (n = 46) with low vision devices. Low vision devices were indicated in a total of 50 students (35.7%). The locally manufactured devices could meet two thirds of the need. A corrected distance acuity of 1/60 or better had a sensitivity of 99.1% and a specificity of 56.7% in predicting the ability to discern N8 print or better. The ability to perform at least two of the three simple tests of functional vision had a sensitivity of 95.5% and a specificity of 63.3% in identifying the students able to discern N8 or better.

Delay of constant light-induced persistent vaginal estrus by 24-hour time cues in rats.

PubMed

Weber, A L; Adler, N T

1979-04-20

The normal ovarian cycle of female rats is typically replaced by persistent estrus when these animals are housed under constant light. Evidence presented here shows that the maintenance of periodicity in the environment can at least delay (if not prevent) the photic induction of persistent vaginal estrus. Female rats in constant light were exposed to vaginal smearing at random times or at the same time every day. In another experiment, female rats were exposed to either constant bright light, constant dim light, or a 24-hour photic cycle of bright and dim light. The onset of persistent vaginal estrus was delayed in rats exposed to 24-hour time cues even though the light intensities were the same as or greater than those for the aperiodic control groups. The results suggest that the absence of 24-hour time cues in constant light contributes to the induction of persistent estrus.

Effect of meal portion size choice on plate waste generation among patients with different nutritional status. An investigation using Dietary Intake Monitoring System (DIMS).

PubMed

Ofei, K T; Holst, M; Rasmussen, H H; Mikkelsen, B E

2015-08-01

The trolley meal system allows hospital patients to select food items and portion sizes directly from the food trolley. The nutritional status of the patient may be compromised if portions selected do not meet recommended intakes for energy, protein and micronutrients. The aim of this study was to investigate: (1) the portion size served, consumed and plate waste generated, (2) the extent to which the size of meal portions served contributes to daily recommended intakes for energy and protein, (3) the predictive effect of the served portion sizes on plate waste in patients screened for nutritional risk by NRS-2002, and (4) to establish the applicability of the dietary intake monitoring system (DIMS) as a technique to monitor plate waste. A prospective observational cohort study was conducted in two hospital wards over five weekdays. The DIMS was used to collect paired before- and after-meal consumption photos and measure the weight of plate content. The proportion of energy and protein consumed by both groups at each meal session could contribute up to 15% of the total daily recommended intake. Linear mixed model identified a positive relationship between meal portion size and plate waste (P = 0.002) and increased food waste in patients at nutritional risk during supper (P = 0.001). Meal portion size was associated with the level of plate waste produced. Being at nutritional risk further increased the extent of waste, regardless of the portion size served at supper. The use of DIMS as an innovative technique might be a promising way to monitor plate waste for optimizing meal portion size servings and minimizing food waste. Copyright © 2015 Elsevier Ltd. All rights reserved.

Clinical colour vision tests.

PubMed

Dain, Stephen J

2004-07-01

The structure and function of the available and significant clinical colour vision tests are reviewed in the light of the needs in the clinical examination of congenital and acquired colour vision deficiencies. The tests are grouped and described as pseudo-isochromatic plates, arrangement tests, matching tests and vocational tests. The colorimetric constructions of the test types are described and the efficiency of their performance and usefulness discussed. Recommendations are made for basic and extended test batteries, when examining of congenital and acquired colour vision deficiencies in the consulting room.

Coping with copepods: do right whales (Eubalaena glacialis) forage visually in dark waters?

PubMed Central

Fasick, Jeffry I.; Kezmoh, Lorren J.; Baumgartner, Mark F.

2017-01-01

North Atlantic right whales (Eubalaena glacialis) feed during the spring and early summer in marine waters off the northeast coast of North America. Their food primarily consists of planktonic copepods, Calanus finmarchicus, which they consume in large numbers by ram filter feeding. The coastal waters where these whales forage are turbid, but they successfully locate copepod swarms during the day at depths exceeding 100 m, where light is very dim and copepod patches may be difficult to see. Using models of E. glacialis visual sensitivity together with measurements of light in waters near Cape Cod where they feed and of light attenuation by living copepods in seawater, we evaluated the potential for visual foraging by these whales. Our results suggest that vision may be useful for finding copepod patches, particularly if E. glacialis searches overhead for silhouetted masses or layers of copepods. This should permit the whales to locate C. finmarchicus visually throughout most daylight hours at depths throughout their foraging range. Looking laterally, the whales might also be able to see copepod patches at short range near the surface. This article is part of the themed issue ‘Vision in dim light’. PMID:28193812

The evolution of rod photoreceptors.

PubMed

Morshedian, Ala; Fain, Gordon L

2017-04-05

Photoreceptors in animals are generally of two kinds: the ciliary or c-type and the rhabdomeric or r-type. Although ciliary photoreceptors are found in many phyla, vertebrates seem to be unique in having two distinct kinds which together span the entire range of vision, from single photons to bright light. We ask why the principal photoreceptors of vertebrates are ciliary and not rhabdomeric, and how rods evolved from less sensitive cone-like photoreceptors to produce our duplex retina. We suggest that the principal advantage of vertebrate ciliary receptors is that they use less ATP than rhabdomeric photoreceptors. This difference may have provided sufficient selection pressure for the development of a completely ciliary eye. Although many of the details of rod evolution are still uncertain, present evidence indicates that (i) rods evolved very early before the split between the jawed and jawless vertebrates, (ii) outer-segment discs make no contribution to rod sensitivity but may have evolved to increase the efficiency of protein renewal, and (iii) evolution of the rod was incremental and multifaceted, produced by the formation of several novel protein isoforms and by changes in protein expression, with no one alteration having more than a few-fold effect on transduction activation or inactivation.This article is part of the themed issue 'Vision in dim light'. © 2017 The Author(s).

Processing of single-photon responses in the mammalian On and Off retinal pathways at the sensitivity limit of vision.

PubMed

Takeshita, Daisuke; Smeds, Lina; Ala-Laurila, Petri

2017-04-05

Visually guided behaviour at its sensitivity limit relies on single-photon responses originating in a small number of rod photoreceptors. For decades, researchers have debated the neural mechanisms and noise sources that underlie this striking sensitivity. To address this question, we need to understand the constraints arising from the retinal output signals provided by distinct retinal ganglion cell types. It has recently been shown in the primate retina that On and Off parasol ganglion cells, the cell types likely to underlie light detection at the absolute visual threshold, differ fundamentally not only in response polarity, but also in the way they handle single-photon responses originating in rods. The On pathway provides the brain with a thresholded, low-noise readout and the Off pathway with a noisy, linear readout. We outline the mechanistic basis of these different coding strategies and analyse their implications for detecting the weakest light signals. We show that high-fidelity, nonlinear signal processing in the On pathway comes with costs: more single-photon responses are lost and their propagation is delayed compared with the Off pathway. On the other hand, the responses of On ganglion cells allow better intensity discrimination compared with the Off ganglion cell responses near visual threshold.This article is part of the themed issue 'Vision in dim light'. © 2017 The Authors.

Suppression of Melatonin Secretion in Totally Visually Blind People by Ocular Exposure to White Light: Clinical Characteristics.

PubMed

Hull, Joseph T; Czeisler, Charles A; Lockley, Steven W

2018-04-03

Although most totally visually blind individuals exhibit nonentrained circadian rhythms due to an inability of light to entrain the circadian pacemaker, a small proportion retain photic circadian entrainment, melatonin suppression, and other nonimage-forming responses to light. It is thought that these responses to light persist because of the survival of melanospin-containing intrinsically photosensitive retinal ganglion cells (ipRGCs), which project primarily to the circadian pacemaker and are functionally distinct from the rod and cone photoreceptors that mediate vision. We aimed to assess the integrity of nonimage-forming photoreception in totally visually blind patients with a range of ocular disorders. Within-subject, dark-controlled design. A total of 18 totally visually blind individuals (7 females; mean age ± standard deviation = 49.8±11.0 years) with various causes of blindness, including 3 bilaterally enucleated controls. Melatonin concentrations were compared during exposure to a 6.5-hour bright white light (∼7000 lux) with melatonin concentrations measured 24 hours earlier at the corresponding clock times under dim-light (4 lux) conditions. Area under the curve (AUC) for melatonin concentration. Melatonin concentrations were significantly suppressed (defined as ≥33% suppression) during the bright-light condition compared with the dim-light condition in 5 of 15 participants with eyes (retinitis pigmentosa, n = 2; retinopathy of prematurity [ROP], n = 2; bilateral retinal detachments, n = 1). Melatonin concentrations remained unchanged in response to light in the remaining 10 participants with eyes (ROP, n = 3; optic neuritis/neuropathy, n = 2; retinopathy unknown, n = 2; congenital glaucoma, n = 1; congenital rubella syndrome, n = 1; measles retinopathy, n = 1) and in all 3 bilaterally enucleated participants. These data confirm that light-induced suppression of melatonin remains functionally intact in a minority of totally visually

Histone H3 lysine 9 methyltransferase FvDim5 regulates fungal development, pathogenicity and osmotic stress responses in Fusarium verticillioides.

PubMed

Gu, Qin; Ji, Tiantian; Sun, Xiao; Huang, Hai; Zhang, Hao; Lu, Xi; Wu, Liming; Huo, Rong; Wu, Huijun; Gao, Xuewen

2017-10-16

Histone methylation plays important biological roles in eukaryotic cells. Methylation of lysine 9 at histone H3 (H3K9me) is critical for regulating chromatin structure and gene transcription. Dim5 is a lysine histone methyltransferase (KHMTase) enzyme, which is responsible for the methylation of H3K9 in eukaryotes. In the current study, we identified a single ortholog of Neurospora crassa Dim5 in Fusarium verticillioides. In this study, we report that FvDim5 regulates the trimethylation of H3K9 (H3K9me3). The FvDIM5 deletion mutant (ΔFvDim5) showed significant defects in conidiation, perithecium production and fungal virulence. Unexpectedly, we found that deletion of FvDIM5 resulted in increased tolerance to osmotic stresses and upregulated FvHog1 phosphorylation. These results indicate the importance of FvDim5 for the regulation of fungal development, pathogenicity and osmotic stress responses in F. verticillioides. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets

PubMed Central

Mao, Hsiaoyin C.; Wei, Min; Hughes, Tiffany; Zhang, Jianying; Park, Il-kyoo; Liu, Shujun; McClory, Susan; Marcucci, Guido; Trotta, Rossana

2010-01-01

Human CD56bright natural killer (NK) cells possess little or no killer immunoglobulin-like receptors (KIRs), high interferon-γ (IFN-γ) production, but little cytotoxicity. CD56dim NK cells have high KIR expression, produce little IFN-γ, yet display high cytotoxicity. We hypothesized that, if human NK maturation progresses from a CD56bright to a CD56dim phenotype, an intermediary NK cell must exist, which demonstrates more functional overlap than these 2 subsets, and we used CD94 expression to test our hypothesis. CD94highCD56dim NK cells express CD62L, CD2, and KIR at levels between CD56bright and CD94lowCD56dim NK cells. CD94highCD56dim NK cells produce less monokine-induced IFN-γ than CD56bright NK cells but much more than CD94lowCD56dim NK cells because of differential interleukin-12–mediated STAT4 phosphorylation. CD94highCD56dim NK cells possess a higher level of granzyme B and perforin expression and CD94-mediated redirected killing than CD56bright NK cells but lower than CD94lowCD56dim NK cells. Collectively, our data suggest that the density of CD94 surface expression on CD56dim NK cells identifies a functional and likely developmental intermediary between CD56bright and CD94lowCD56dim NK cells. This supports the notion that, in vivo, human CD56bright NK cells progress through a continuum of differentiation that ends with a CD94lowCD56dim phenotype. PMID:19897577

CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets.

PubMed

Yu, Jianhua; Mao, Hsiaoyin C; Wei, Min; Hughes, Tiffany; Zhang, Jianying; Park, Il-kyoo; Liu, Shujun; McClory, Susan; Marcucci, Guido; Trotta, Rossana; Caligiuri, Michael A

2010-01-14

Human CD56(bright) natural killer (NK) cells possess little or no killer immunoglobulin-like receptors (KIRs), high interferon-gamma (IFN-gamma) production, but little cytotoxicity. CD56(dim) NK cells have high KIR expression, produce little IFN-gamma, yet display high cytotoxicity. We hypothesized that, if human NK maturation progresses from a CD56(bright) to a CD56(dim) phenotype, an intermediary NK cell must exist, which demonstrates more functional overlap than these 2 subsets, and we used CD94 expression to test our hypothesis. CD94(high)CD56(dim) NK cells express CD62L, CD2, and KIR at levels between CD56(bright) and CD94(low)CD56(dim) NK cells. CD94(high)CD56(dim) NK cells produce less monokine-induced IFN-gamma than CD56(bright) NK cells but much more than CD94(low)CD56(dim) NK cells because of differential interleukin-12-mediated STAT4 phosphorylation. CD94(high)CD56(dim) NK cells possess a higher level of granzyme B and perforin expression and CD94-mediated redirected killing than CD56(bright) NK cells but lower than CD94(low)CD56(dim) NK cells. Collectively, our data suggest that the density of CD94 surface expression on CD56(dim) NK cells identifies a functional and likely developmental intermediary between CD56(bright) and CD94(low)CD56(dim) NK cells. This supports the notion that, in vivo, human CD56(bright) NK cells progress through a continuum of differentiation that ends with a CD94(low)CD56(dim) phenotype.

What Aspects of Vision Facilitate Haptic Processing?

ERIC Educational Resources Information Center

Millar, Susanna; Al-Attar, Zainab

2005-01-01

We investigate how vision affects haptic performance when task-relevant visual cues are reduced or excluded. The task was to remember the spatial location of six landmarks that were explored by touch in a tactile map. Here, we use specially designed spectacles that simulate residual peripheral vision, tunnel vision, diffuse light perception, and…

Conformational changes and metastable states induced in proteins by green light

NASA Astrophysics Data System (ADS)

Comorosan, Sorin; Popescu, Irinel; Polosan, Silviu; Pirvu, Cristian; Ionescu, Elena; Paslaru, Liliana; Apostol, Marian

2015-01-01

In this paper we report conformational changes recorded on a protein molecule (α-amylase) under green light irradiation. In order to explain the experimental results we advanced the hypothesis that green light induces electric dipoles in the protein, which interact with each other, generating conformational modifications toward a more compact design, with different physical properties. The experiments were carried out with un-polarized light (λ = 520 nm) from a light-emitting-diode (1000 lm, 20 W, 105 mW on the target). In view of the character of our hypothesis, and corroborated with all our experimental results, we suggest that this phenomenon may be more extended and general, specific for a larger class of proteins, occurring on the protein macromolecules under the green light. The effects of α-amylase protein irradiation were revealed by circular dichroism, fluorescence, Raman and FTIR-spectroscopies, zeta potential, cyclic voltammetry, electric impedance spectroscopy and atomic force microscopy. Tentatively, we term the novel conformations as P∗ (polarized) proteins.

Vision-Related Quality of Life Associated with Unilateral and Bilateral Ocular Conditions.

PubMed

Brown, Gary C; Brown, Melissa M; Stein, Joshua D; Smiddy, William E

2018-02-21

To present ophthalmic patient time-tradeoff vision utilities for quantifying vision-related quality-of-life when the fellow eye still has good vision. These utilities are important for performing reliable cost-utility analyses. Consecutive time-tradeoff vision utilities were obtained from ophthalmic patients with good vision (20/20-20/25) in one eye and vision ranging from 20/20 to no light perception in the fellow eye over a 15-year period from 2000 through 2014. Five hundred eighty-six ophthalmic participant interviews from Wills Eye Hospital, New York Eye and Ear Hospital, and ophthalmology office practices in Pennsylvania and New Jersey. Participants underwent a full ophthalmic examination, after which time-tradeoff vision utilities were obtained by personal interview by the authors using a standardized, validated instrument. Time-tradeoff vision utilities. Mean time-tradeoff vision utilities were as follows in participants with good vision (20/20-20/25) in at least one eye and the following visions in the fellow eyes: no light perception, 0.79; counting fingers to light perception, 0.87; 20/200 to 20/400, 0.88; 20/60 to 20/100, 0.88; 20/30 to 20/50, 0.87; and 20/20 to 20/25, 0.94. In people with good vision (20/20-20/25) in one eye, the associated mean time-tradeoff vision utility is a remarkably consistent 0.87 to 0.88 when vision in the fellow eye ranges from 20/30 to light perception. Vision of 20/20 to 20/25 in the fellow eye results in a significantly higher associated utility of 0.94 (P < 0.01), whereas vision of no light perception in the fellow eye results in a significantly lower utility of 0.079 (P < 0.01). These utilities are important for calculating reliable patient value (quality-adjusted life-year) gains in ophthalmic cost-utility analysis populations in which there is unilateral and bilateral disease involvement. Copyright © 2018 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

The photochemical determinants of color vision: revealing how opsins tune their chromophore's absorption wavelength.

PubMed

Wang, Wenjing; Geiger, James H; Borhan, Babak

2014-01-01

The evolution of a variety of important chromophore-dependent biological processes, including microbial light sensing and mammalian color vision, relies on protein modifications that alter the spectral characteristics of a bound chromophore. Three different color opsins share the same chromophore, but have three distinct absorptions that together cover the entire visible spectrum, giving rise to trichromatic vision. The influence of opsins on the absorbance of the chromophore has been studied through methods such as model compounds, opsin mutagenesis, and computational modeling. The recent development of rhodopsin mimic that uses small soluble proteins to recapitulate the binding and wavelength tuning of the native opsins provides a new platform for studying protein-regulated spectral tuning. The ability to achieve far-red shifted absorption in the rhodopsin mimic system was attributed to a combination of the lack of a counteranion proximal to the iminium, and a uniformly neutral electrostatic environment surrounding the chromophore. © 2014 WILEY Periodicals, Inc.

The visual ecology of a deep-sea fish, the escolar Lepidocybium flavobrunneum (Smith, 1843)†

PubMed Central

Landgren, Eva; Fritsches, Kerstin; Brill, Richard; Warrant, Eric

2014-01-01

Escolar (Lepidocybium flavobrunneum, family Gempylidae) are large and darkly coloured deep-sea predatory fish found in the cold depths (more than 200 m) during the day and in warm surface waters at night. They have large eyes and an overall low density of retinal ganglion cells that endow them with a very high optical sensitivity. Escolar have banked retinae comprising six to eight layers of rods to increase the optical path length for maximal absorption of the incoming light. Their retinae possess two main areae of higher ganglion cell density, one in the ventral retina viewing the dorsal world above (with a moderate acuity of 4.6 cycles deg−1), and the second in the temporal retina viewing the frontal world ahead. Electrophysiological recordings of the flicker fusion frequency (FFF) in isolated retinas indicate that escolar have slow vision, with maximal FFF at the highest light levels and temperatures (around 9 Hz at 23°C) which fall to 1–2 Hz in dim light or cooler temperatures. Our results suggest that escolar are slowly moving sit-and-wait predators. In dim, warm surface waters at night, their slow vision, moderate dorsal resolution and highly sensitive eyes may allow them to surprise prey from below that are silhouetted in the downwelling light. PMID:24395966

The Impact of Vision in Spatial Coding

ERIC Educational Resources Information Center

Papadopoulos, Konstantinos; Koustriava, Eleni

2011-01-01

The aim of this study is to examine the performance in coding and representing of near-space in relation to vision status (blindness vs. normal vision) and sensory modality (touch vs. vision). Forty-eight children and teenagers participated. Sixteen of the participants were totally blind or had only light perception, 16 were blindfolded sighted…

Protection of Hawaii's Observatories from Light Pollution

NASA Astrophysics Data System (ADS)

Wainscoat, Richard J.

2018-01-01

Maunakea Observatory, located on the island of Hawaii, is among the world darkest sites for astronomy. Strong efforts to preserve the dark night sky over the last forty years have proven successful. Artificial light presently adds only approximately 2% to the natural night sky brightness. The techniques being used to protect Maunakea from light pollution will be described, along with the challenges that are now being faced.Haleakala Observatory, located on the island of Maui, is also an excellent observing site, and is among the best sites in the United States. Lighting restrictions in Maui County are much weaker, and consequently, the night sky above Haleakala is less well protected. Haleakala is closer to Honolulu and the island of Oahu (population approximately 1 million), and the glow from Oahu makes the northwestern sky brighter.Much of the lighting across most of the United States, including Hawaii, is presently being converted to LED lighting. This provides an opportunity to replace existing poorly shielded lights with properly shielded LED fixtures, but careful spectral management is essential. It is critically important to only use LED lighting that is deficient in blue and green light. LED lighting also is easy to dim. Dimming of lights later at night, when there is no need for brighter lighting, is an important tool for reducing light pollution.Techniques used to protect astronomical observatories from light pollution are similar to the techniques that must be used to protect animals that are affected by light at night, such as endangered birds and turtles. These same techniques are compatible with recent human health related lighting recommendations from the American Medical Association.

Advances in understanding the molecular basis of the first steps in color vision

PubMed Central

Hofmann, Lukas; Palczewski, Krzysztof

2015-01-01

Serving as one of our primary environmental inputs, vision is the most sophisticated sensory system in humans. Here, we present recent findings derived from energetics, genetics and physiology that provide a more advanced understanding of color perception in mammals. Energetics of cis–trans isomerization of 11-cis-retinal accounts for color perception in the narrow region of the electromagnetic spectrum and how human eyes can absorb light in the near infrared (IR) range. Structural homology models of visual pigments reveal complex interactions of the protein moieties with the light sensitive chromophore 11-cis-retinal and that certain color blinding mutations impair secondary structural elements of these G protein-coupled receptors (GPCRs). Finally, we identify unsolved critical aspects of color tuning that require future investigation. PMID:26187035

Combination of light and melatonin time cues for phase advancing the human circadian clock.

PubMed

Burke, Tina M; Markwald, Rachel R; Chinoy, Evan D; Snider, Jesse A; Bessman, Sara C; Jung, Christopher M; Wright, Kenneth P

2013-11-01

Photic and non-photic stimuli have been shown to shift the phase of the human circadian clock. We examined how photic and non-photic time cues may be combined by the human circadian system by assessing the phase advancing effects of one evening dose of exogenous melatonin, alone and in combination with one session of morning bright light exposure. Randomized placebo-controlled double-blind circadian protocol. The effects of four conditions, dim light (∼1.9 lux, ∼0.6 Watts/m(2))-placebo, dim light-melatonin (5 mg), bright light (∼3000 lux, ∼7 Watts/m(2))-placebo, and bright light-melatonin on circadian phase was assessed by the change in the salivary dim light melatonin onset (DLMO) prior to and following treatment under constant routine conditions. Melatonin or placebo was administered 5.75 h prior to habitual bedtime and 3 h of bright light exposure started 1 h prior to habitual wake time. Sleep and chronobiology laboratory environment free of time cues. Thirty-six healthy participants (18 females) aged 22 ± 4 y (mean ± SD). Morning bright light combined with early evening exogenous melatonin induced a greater phase advance of the DLMO than either treatment alone. Bright light alone and melatonin alone induced similar phase advances. Information from light and melatonin appear to be combined by the human circadian clock. The ability to combine circadian time cues has important implications for understanding fundamental physiological principles of the human circadian timing system. Knowledge of such principles is important for designing effective countermeasures for phase-shifting the human circadian clock to adapt to jet lag, shift work, and for designing effective treatments for circadian sleep-wakefulness disorders.

Advanced Lighting Controls for Reducing Energy Use and Cost in DoD Installations

DTIC Science & Technology

2013-03-01

of advanced lighting control strategies including occupancy sensing, light tuning, daylight harvesting and proper lighting design is an effective way...details of this project and the results obtained. 15. SUBJECT TERMS Integrated lighting controls, occupancy sensing, daylight harvesting , personalized...provide energy savings through occupancy sensing, dimming and daylight harvesting . II. Dynalite is a distributed control-based, wired networked building

Novel sensor for color control in solid state lighting applications

NASA Astrophysics Data System (ADS)

Gourevitch, Alex; Thurston, Thomas; Singh, Rajiv; Banachowicz, Bartosz; Korobov, Vladimir; Drowley, Cliff

2010-02-01

LED wavelength and luminosity shifts due to temperature, dimming, aging, and binning uncertainty can cause large color errors in open-loop light-mixing illuminators. Multispectral color light sensors combined with feedback circuits can compensate for these LED shifts. Typical color light sensor design variables include the choice of light-sensing material, filter configuration, and read-out circuitry. Cypress Semiconductor has designed and prototyped a color sensor chip that consists of photodiode arrays connected to a I/F (Current to Frequency) converter. This architecture has been chosen to achieve high dynamic range (~100dB) and provide flexibility for tailoring sensor response. Several different optical filter configurations were evaluated in this prototype. The color-sensor chip was incorporated into an RGB light color mixing system with closed-loop optical feedback. Color mixing accuracy was determined by calculating the difference between (u',v') set point values and CIE coordinates measured with a reference colorimeter. A typical color precision ▵u'v' less than 0.0055 has been demonstrated over a wide range of colors, a temperature range of 50C, and light dimming up to 80%.

Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats

PubMed Central

Chen, Hui; Tran, Julie-Thu A.; Anderson, Robert E.

2012-01-01

Purpose Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H2O2-mediated cell death and in albino rats (in vivo) against various light conditions. Methods The 661W cells were pretreated with CAPE and then stressed with H2O2. Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding. Results Pretreatment of 661W cells with CAPE reduced H2O2-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light. Conclusions CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats’ retinas. PMID:22690111

Regulation of macroautophagy in ovarian cancer cells in vitro and in vivo by controlling Glucose regulatory protein 78 and AMPK

PubMed Central

Kandala, Prabodh K.; Srivastava, Sanjay K.

2012-01-01

In this study we show that diindolylmethane (DIM) induces autophagy in ovarian cancer cells by regulating endoplasmic reticulum (ER) stress and AMPK. Treatment of SKOV-3, OVCAR-3 and TOV-21G ovarian cancer cells with varying concentrations of DIM for 24 hours resulted in a concentration dependent induction of autophagy as measured by flowcytometry. Electron microscopy confirmed the presence of autophagosomes in DIM treated cells. Western blot analysis showed that DIM treatment increased the expression of LC3B, a hall mark of autophagy as well as p62 and Atg 12 proteins that are accumulated during autophagy. Autophagy inhibitors bafilomycin or chloroquine inhibited DIM induced autophagy. Furthermore, DIM treatment significantly increased the expression of ER stress regulators such as Grp78, IRE1 and GADD153. Cycloheximide or ER stress inhibitor mithramycin not only blocked ER stress proteins that were activated by DIM but also autophagy. Silencing Grp78 or GADD 153 significantly blocked the expression of LC3B and p62 indicating that autophagy in our model is mediated by ER stress. Knocking out LC3B inhibited DIM induced autophagy. DIM treatment increased the cytosolic calcium levels which lead to the activation of AMPK in our model. Chelating cytosolic calcium with BAPT-AM abrogated not only the phosphorylation of AMPK but also prevented DIM induced autophagy. Inhibiting AMPK by a chemical inhibitor or siRNA blocked the induction of LC3B or p62, indicating that DIM mediated autophagy requires activation of AMPK. Oral administration of DIM significantly suppressed SKOV-3 tumor xenografts in nude mice. Activation of ER stress and autophagy were observed in the tumors of DIM treated mice. Taken together, these results suggest that induction of autophagy by DIM in ovarian cancer cells was associated with ER stress and AMPK activation. PMID:22564965

Robotic vision. [process control applications

NASA Technical Reports Server (NTRS)

Williams, D. S.; Wilf, J. M.; Cunningham, R. T.; Eskenazi, R.

1979-01-01

Robotic vision, involving the use of a vision system to control a process, is discussed. Design and selection of active sensors employing radiation of radio waves, sound waves, and laser light, respectively, to light up unobservable features in the scene are considered, as are design and selection of passive sensors, which rely on external sources of illumination. The segmentation technique by which an image is separated into different collections of contiguous picture elements having such common characteristics as color, brightness, or texture is examined, with emphasis on the edge detection technique. The IMFEX (image feature extractor) system performing edge detection and thresholding at 30 frames/sec television frame rates is described. The template matching and discrimination approach to recognize objects are noted. Applications of robotic vision in industry for tasks too monotonous or too dangerous for the workers are mentioned.

Unequal error control scheme for dimmable visible light communication systems

NASA Astrophysics Data System (ADS)

Deng, Keyan; Yuan, Lei; Wan, Yi; Li, Huaan

2017-01-01

Visible light communication (VLC), which has the advantages of a very large bandwidth, high security, and freedom from license-related restrictions and electromagnetic-interference, has attracted much interest. Because a VLC system simultaneously performs illumination and communication functions, dimming control, efficiency, and reliable transmission are significant and challenging issues of such systems. In this paper, we propose a novel unequal error control (UEC) scheme in which expanding window fountain (EWF) codes in an on-off keying (OOK)-based VLC system are used to support different dimming target values. To evaluate the performance of the scheme for various dimming target values, we apply it to H.264 scalable video coding bitstreams in a VLC system. The results of the simulations that are performed using additive white Gaussian noises (AWGNs) with different signal-to-noise ratios (SNRs) are used to compare the performance of the proposed scheme for various dimming target values. It is found that the proposed UEC scheme enables earlier base layer recovery compared to the use of the equal error control (EEC) scheme for different dimming target values and therefore afford robust transmission for scalable video multicast over optical wireless channels. This is because of the unequal error protection (UEP) and unequal recovery time (URT) of the EWF code in the proposed scheme.

Clinical Tests of Ultra-Low Vision Used to Evaluate Rudimentary Visual Perceptions Enabled by the BrainPort Vision Device.

PubMed

Nau, Amy; Bach, Michael; Fisher, Christopher

2013-01-01

We evaluated whether existing ultra-low vision tests are suitable for measuring outcomes using sensory substitution. The BrainPort is a vision assist device coupling a live video feed with an electrotactile tongue display, allowing a user to gain information about their surroundings. We enrolled 30 adult subjects (age range 22-74) divided into two groups. Our blind group included 24 subjects ( n = 16 males and n = 8 females, average age 50) with light perception or worse vision. Our control group consisted of six subjects ( n = 3 males, n = 3 females, average age 43) with healthy ocular status. All subjects performed 11 computer-based psychophysical tests from three programs: Basic Assessment of Light Motion, Basic Assessment of Grating Acuity, and the Freiburg Vision Test as well as a modified Tangent Screen. Assessments were performed at baseline and again using the BrainPort after 15 hours of training. Most tests could be used with the BrainPort. Mean success scores increased for all of our tests except contrast sensitivity. Increases were statistically significant for tests of light perception (8.27 ± 3.95 SE), time resolution (61.4% ± 3.14 SE), light localization (44.57% ± 3.58 SE), grating orientation (70.27% ± 4.64 SE), and white Tumbling E on a black background (2.49 logMAR ± 0.39 SE). Motion tests were limited by BrainPort resolution. Tactile-based sensory substitution devices are amenable to psychophysical assessments of vision, even though traditional visual pathways are circumvented. This study is one of many that will need to be undertaken to achieve a common outcomes infrastructure for the field of artificial vision.

Indoor visible light communication with smart lighting technology

NASA Astrophysics Data System (ADS)

Das Barman, Abhirup; Halder, Alak

2017-02-01

An indoor visible-light communication performance is investigated utilizing energy efficient white light by 2D LED arrays. Enabled by recent advances in LED technology, IEEE 802.15.7 standardizes high-data-rate visible light communication and advocates for colour shift keying (CSK) modulation to overcome flicker and to support dimming. Voronoi segmentation is employed for decoding N-CSK constellation which has superior performance compared to other existing decoding methods. The two chief performance degrading effects of inter-symbol interference and LED nonlinearity is jointly mitigated using LMS post equalization at the receiver which improves the symbol error rate performance and increases field of view of the receiver. It is found that LMS post equalization symbol at 250MHz offers 7dB SNR improvement at SER10-6

Your Child's Vision

MedlinePlus

... 3½, kids should have eye health screenings and visual acuity tests (tests that measure sharpness of vision) ... eye rubbing extreme light sensitivity poor focusing poor visual tracking (following an object) abnormal alignment or movement ...

Secular dimming of KIC 8462852 following its consumption of a planet

NASA Astrophysics Data System (ADS)

Metzger, Brian D.; Shen, Ken J.; Stone, Nicholas

2017-07-01

The Kepler-field star KIC 8462852, an otherwise apparently ordinary F3 main-sequence star, showed several highly unusual dimming events of variable depth and duration. Adding to the mystery was the discovery that KIC 8462852 faded by 14 per cent from 1890 to 1989, as well as by another 3 per cent over the 4 yr Kepler mission. Following an initial suggestion by Wright & Sigurdsson, we propose that the secular dimming behaviour is the result of the inspiral of a planetary body or bodies into KIC 8462852, which took place ˜10-104 yr ago (depending on the planet mass). Gravitational energy released as the body inspirals into the outer layers of the star caused a temporary and unobserved brightening, from which the stellar flux is now returning to the quiescent state. The transient dimming events could then be due to obscuration by planetary debris from an earlier partial disruption of the same inspiralling bodies, or due to evaporation and outgassing from a tidally detached moon system. Alternatively, the dimming events could arise from a large number of comet- or planetesimal-mass bodies placed on to high-eccentricity orbits by the same mechanism (e.g. Lidov-Kozai oscillations due to the outer M-dwarf companion) responsible for driving the more massive planets into KIC 8462852. The required high occurrence rate of KIC 8462852-like systems that have undergone recent major planet inspiral event(s) is the greatest challenge to the model, placing large lower limits on the mass of planetary systems surrounding F stars and/or requiring an unlikely probability to catch KIC 8462852 in its current state.

The family of light-harvesting-related proteins (LHCs, ELIPs, HLIPs): was the harvesting of light their primary function?

PubMed

Montané, M H; Kloppstech, K

2000-11-27

Light-harvesting complex proteins (LHCs) and early light-induced proteins (ELIPs) are essential pigment-binding components of the thylakoid membrane and are encoded by one of the largest and most complex higher plant gene families. The functional diversification of these proteins corresponded to the transition from extrinsic (phycobilisome-based) to intrinsic (LHC-based) light-harvesting antenna systems during the evolution of chloroplasts from cyanobacteria, yet the functional basis of this diversification has been elusive. Here, we propose that the original function of LHCs and ELIPs was not to collect light and to transfer its energy content to the reaction centers but to disperse the absorbed energy of light in the form of heat or fluorescence. These energy-dispersing proteins are believed to have originated in cyanobacteria as one-helix, highly light-inducible proteins (HLIPs) that later acquired four helices through two successive gene duplication steps. We suggest that the ELIPs arose first in this succession, with a primary function in energy dispersion for protection of photosynthetic pigments from photo-oxidation. We consider the LHC I and II families as more recent and very successful evolutionary additions to this family that ultimately attained a new function, thereby replacing the ancestral extrinsic light-harvesting system. Our model accounts for the non-photochemical quenching role recently shown for higher plant psbS proteins.

Dark Matters: Challenges of Nocturnal Communication Between Plants and Animals in Delivery of Pollination Services

PubMed Central

Borges, Renee M.

2018-01-01

The night is a special niche characterized by dim light, lower temperatures, and higher humidity compared to the day. Several animals have made the transition from the day into the night and have acquired unique adaptations to cope with the challenges of performing nocturnal activities. Several plant species have opted to bloom at night, possibly as a response to aridity to prevent excessive water loss through evapotranspiration since flowering is often a water-demanding process, or to protect pollen from heat stress. Nocturnal pollinators have visual adaptations to function under dim light conditions but may also trade off vision against olfaction when they are dependent on nectar-rewarding and scented flowers. Nocturnal pollinators may use CO2 and humidity cues emanating from freshly-opened flowers as indicators of nectar-rich resources. Some endothermic nocturnal insect pollinators are attracted to thermogenic flowers within which they remain to obtain heat as a reward to increase their energy budget. This review focuses on mechanisms that pollinators use to find flowers at night, and the signals that nocturnally blooming flowers may employ to attract pollinators under dim light conditions. It also indicates gaps in our knowledge. While millions of years of evolutionary time have given pollinators and plants solutions to the delivery of pollination services and to the offering of appropriate rewards, this history of successful evolution is being threatened by artificial light at night. Excessive and inappropriate illumination associated with anthropogenic activities has resulted in significant light pollution which serves to undermine life processes governed by dim light. PMID:29599655

Dark Matters: Challenges of Nocturnal Communication Between Plants and Animals in Delivery of Pollination Services.

PubMed

Borges, Renee M

2018-03-01

The night is a special niche characterized by dim light, lower temperatures, and higher humidity compared to the day. Several animals have made the transition from the day into the night and have acquired unique adaptations to cope with the challenges of performing nocturnal activities. Several plant species have opted to bloom at night, possibly as a response to aridity to prevent excessive water loss through evapotranspiration since flowering is often a water-demanding process, or to protect pollen from heat stress. Nocturnal pollinators have visual adaptations to function under dim light conditions but may also trade off vision against olfaction when they are dependent on nectar-rewarding and scented flowers. Nocturnal pollinators may use CO 2 and humidity cues emanating from freshly-opened flowers as indicators of nectar-rich resources. Some endothermic nocturnal insect pollinators are attracted to thermogenic flowers within which they remain to obtain heat as a reward to increase their energy budget. This review focuses on mechanisms that pollinators use to find flowers at night, and the signals that nocturnally blooming flowers may employ to attract pollinators under dim light conditions. It also indicates gaps in our knowledge. While millions of years of evolutionary time have given pollinators and plants solutions to the delivery of pollination services and to the offering of appropriate rewards, this history of successful evolution is being threatened by artificial light at night. Excessive and inappropriate illumination associated with anthropogenic activities has resulted in significant light pollution which serves to undermine life processes governed by dim light.

Pulsed-light imaging for fluorescence guided surgery under normal room lighting

PubMed Central

Sexton, Kristian; Davis, Scott C.; McClatchy, David; Valdes, Pablo A.; Kanick, Stephen C.; Paulsen, Keith D.; Roberts, David W.; Pogue, Brian W.

2013-01-01

Fluorescence guided surgery (FGS) is an emerging technology that has demonstrated improved surgical outcomes. However, dim lighting conditions required bycurrent FGS systems are disruptive to standard surgical workflow. We present a novel FGS system capable of imaging fluorescence under normal room lightby using pulsed excitation and gated acquisition. Images from tissue-simulating phantoms confirm visual detection down to 0.25 μM of protopor-phyrin IX under 125 μW/cm2 of ambient light, more than an order of magnitude lower than that measured with the Zeiss Pentero in the dark. Resection of orthotopic brain tumors in mice also suggests that the pulsed-light system provides superior sensitivity in vivo. PMID:23988926

Subjective Perception of Visual Distortions or Scotomas in Individuals with Retinitis Pigmentosa

ERIC Educational Resources Information Center

Wittich, Walter; Watanabe, Donald H.; Kapusta, Michael A.; Overbury, Olga

2011-01-01

It is often assumed that persons who develop ocular disease have some form of visual experience that makes them aware of their deficits. However, in the case of peripheral field loss or decreasing vision in dim lighting, as in retinitis pigmentosa, for example, symptoms are more obscure and may not be as easily identified by the persons who have…

Plants Actively Avoid State Transitions upon Changes in Light Intensity: Role of Light-Harvesting Complex II Protein Dephosphorylation in High Light1[OPEN

PubMed Central

Suorsa, Marjaana; Rantala, Marjaana; Aro, Eva-Mari

2015-01-01

Photosystem II (PSII) core and light-harvesting complex II (LHCII) proteins in plant chloroplasts undergo reversible phosphorylation upon changes in light intensity (being under control of redox-regulated STN7 and STN8 kinases and TAP38/PPH1 and PSII core phosphatases). Shift of plants from growth light to high light results in an increase of PSII core phosphorylation, whereas LHCII phosphorylation concomitantly decreases. Exactly the opposite takes place when plants are shifted to lower light intensity. Despite distinct changes occurring in thylakoid protein phosphorylation upon light intensity changes, the excitation balance between PSII and photosystem I remains unchanged. This differs drastically from the canonical-state transition model induced by artificial states 1 and 2 lights that concomitantly either dephosphorylate or phosphorylate, respectively, both the PSII core and LHCII phosphoproteins. Analysis of the kinase and phosphatase mutants revealed that TAP38/PPH1 phosphatase is crucial in preventing state transition upon increase in light intensity. Indeed, tap38/pph1 mutant revealed strong concomitant phosphorylation of both the PSII core and LHCII proteins upon transfer to high light, thus resembling the wild type under state 2 light. Coordinated function of thylakoid protein kinases and phosphatases is shown to secure balanced excitation energy for both photosystems by preventing state transitions upon changes in light intensity. Moreover, PROTON GRADIENT REGULATION5 (PGR5) is required for proper regulation of thylakoid protein kinases and phosphatases, and the pgr5 mutant mimics phenotypes of tap38/pph1. This shows that there is a close cooperation between the redox- and proton gradient-dependent regulatory mechanisms for proper function of the photosynthetic machinery. PMID:25902812

A period (per)-like protein exhibits daily rhythmicity in the suprachiasmatic nuclei of the rat.

PubMed

Rosewell, K L; Siwicki, K K; Wise, P M

1994-10-03

The period (per) gene of Drosophila melanogaster is considered an important biological clock gene, since it regulates multiple behavioral rhythms. Per mRNA and protein exhibit circadian rhythms in the fruitfly brain and these rhythms appear to influence each other through a feedback loop. More recently, using the same antibody as was used in the Drosophila studies, PER-like proteins were detected in the suprachiasmatic nuclei (SCN) of male rats. This region of the brain is considered to be a major neural circadian pacemaker in mammals. The purpose of this study was to confirm that PER-like proteins are detectable in the SCN of female rats and to determine whether PER-like proteins exhibit a circadian rhythm. Female rats were killed at several times of day under both light/dark and constant conditions. Using the same anti-PER antibody in Western blots with Enhanced Chemiluminescence (Western-ECL) detection, the levels of the PER-like proteins were quantified in the SCN and cerebral cortex. The antibody identified a doublet band of approximately 170-160 kDa and a single band at 115 kDa. Of the three PER-like proteins only the largest exhibited a daily rhythm in the SCN, which peaked in the middle of the dark and attained its nadir around lights off; levels during the light were intermediate with a tendency towards a second drop around lights on. This rhythm did not persist under constant dim red light.(ABSTRACT TRUNCATED AT 250 WORDS)

Design and engineering of water-soluble light-harvesting protein maquettes

DOE PAGES

Kodali, Goutham; Mancini, Joshua A.; Solomon, Lee A.; ...

2017-01-01

Natural selection in photosynthesis has engineered tetrapyrrole based, nanometer scale, light harvesting and energy capture in light-induced charge separation. By designing and creating nanometer scale artificial light harvesting and charge separating proteins, we have the opportunity to reengineer and overcome the limitations of natural selection to extend energy capture to new wavelengths and to tailor efficient systems that better meet human as opposed to cellular energetic needs. While tetrapyrrole cofactor incorporation in natural proteins is complex and often assisted by accessory proteins for cofactor transport and insertion, artificial protein functionalization relies on a practical understanding of the basic physical chemistrymore » of protein and cofactors that drive nanometer scale self-assembly. Patterning and balancing of hydrophobic and hydrophilic tetrapyrrole substituents is critical to avoid natural or synthetic porphyrin and chlorin aggregation in aqueous media and speed cofactor partitioning into the non-polar core of a man-made water soluble protein designed according to elementary first principles of protein folding. In conclusion, this partitioning is followed by site-specific anchoring of tetrapyrroles to histidine ligands strategically placed for design control of rates and efficiencies of light energy and electron transfer while orienting at least one polar group towards the aqueous phase.« less

Design and engineering of water-soluble light-harvesting protein maquettes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kodali, Goutham; Mancini, Joshua A.; Solomon, Lee A.

Natural selection in photosynthesis has engineered tetrapyrrole based, nanometer scale, light harvesting and energy capture in light-induced charge separation. By designing and creating nanometer scale artificial light harvesting and charge separating proteins, we have the opportunity to reengineer and overcome the limitations of natural selection to extend energy capture to new wavelengths and to tailor efficient systems that better meet human as opposed to cellular energetic needs. While tetrapyrrole cofactor incorporation in natural proteins is complex and often assisted by accessory proteins for cofactor transport and insertion, artificial protein functionalization relies on a practical understanding of the basic physical chemistrymore » of protein and cofactors that drive nanometer scale self-assembly. Patterning and balancing of hydrophobic and hydrophilic tetrapyrrole substituents is critical to avoid natural or synthetic porphyrin and chlorin aggregation in aqueous media and speed cofactor partitioning into the non-polar core of a man-made water soluble protein designed according to elementary first principles of protein folding. In conclusion, this partitioning is followed by site-specific anchoring of tetrapyrroles to histidine ligands strategically placed for design control of rates and efficiencies of light energy and electron transfer while orienting at least one polar group towards the aqueous phase.« less

Color vision test for dichromatic and trichromatic macaque monkeys.

PubMed

Koida, Kowa; Yokoi, Isao; Okazawa, Gouki; Mikami, Akichika; Widayati, Kanthi Arum; Miyachi, Shigehiro; Komatsu, Hidehiko

2013-11-01

Dichromacy is a color vision defect in which one of the three cone photoreceptors is absent. Individuals with dichromacy are called dichromats (or sometimes "color-blind"), and their color discrimination performance has contributed significantly to our understanding of color vision. Macaque monkeys, which normally have trichromatic color vision that is nearly identical to humans, have been used extensively in neurophysiological studies of color vision. In the present study we employed two tests, a pseudoisochromatic color discrimination test and a monochromatic light detection test, to compare the color vision of genetically identified dichromatic macaques (Macaca fascicularis) with that of normal trichromatic macaques. In the color discrimination test, dichromats could not discriminate colors along the protanopic confusion line, though trichromats could. In the light detection test, the relative thresholds for longer wavelength light were higher in the dichromats than the trichromats, indicating dichromats to be less sensitive to longer wavelength light. Because the dichromatic macaque is very rare, the present study provides valuable new information on the color vision behavior of dichromatic macaques, which may be a useful animal model of human dichromacy. The behavioral tests used in the present study have been previously used to characterize the color behaviors of trichromatic as well as dichromatic new world monkeys. The present results show that comparative studies of color vision employing similar tests may be feasible to examine the difference in color behaviors between trichromatic and dichromatic individuals, although the genetic mechanisms of trichromacy/dichromacy is quite different between new world monkeys and macaques.

Chemosensitization of Breast Cancer Cells to Chemotherapeutic Agents by 3,3’-Diindolylmethane (DIM)

DTIC Science & Technology

2007-08-01

activity (Fig. 4A). More importantly, our animal studies showed that dietary B-DIM in combination with Taxotere also inhibited NF-κB DNA binding...κB DNA-binding activity (Fig. 4C). Importantly, our animal studies showed that dietary B-DIM in combination with Taxotere inhibited NF-κB DNA...Rahman et al 15 DISCUSSION Current reports have shown that plant-derived dietary compounds provide protection against the

Evolutionary replacement of UV vision by violet vision in fish.

PubMed

Tada, Takashi; Altun, Ahmet; Yokoyama, Shozo

2009-10-13

The vertebrate ancestor possessed ultraviolet (UV) vision and many species have retained it during evolution. Many other species switched to violet vision and, then again, some avian species switched back to UV vision. These UV and violet vision are mediated by short wavelength-sensitive (SWS1) pigments that absorb light maximally (lambda(max)) at approximately 360 and 390-440 nm, respectively. It is not well understood why and how these functional changes have occurred. Here, we cloned the pigment of scabbardfish (Lepidopus fitchi) with a lambda(max) of 423 nm, an example of violet-sensitive SWS1 pigment in fish. Mutagenesis experiments and quantum mechanical/molecular mechanical (QM/MM) computations show that the violet-sensitivity was achieved by the deletion of Phe-86 that converted the unprotonated Schiff base-linked 11-cis-retinal to a protonated form. The finding of a violet-sensitive SWS1 pigment in scabbardfish suggests that many other fish also have orthologous violet pigments. The isolation and comparison of such violet and UV pigments in fish living in different ecological habitats will open an unprecedented opportunity to elucidate not only the molecular basis of phenotypic adaptations, but also the genetics of UV and violet vision.

In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light.

PubMed

Hoppmann, Christian; Maslennikov, Innokentiy; Choe, Senyon; Wang, Lei

2015-09-09

Optical modulation of proteins provides superior spatiotemporal resolution for understanding biological processes, and photoswitches built on light-sensitive proteins have been significantly advancing neuronal and cellular studies. Small molecule photoswitches could complement protein-based switches by mitigating potential interference and affording high specificity for modulation sites. However, genetic encodability and responsiveness to nonultraviolet light, two desired properties possessed by protein photoswitches, are challenging to be engineered into small molecule photoswitches. Here we developed a small molecule photoswitch that can be genetically installed onto proteins in situ and controlled by visible light. A pentafluoro azobenzene-based photoswitchable click amino acid (F-PSCaa) was designed to isomerize in response to visible light. After genetic incorporation into proteins via the expansion of the genetic code, F-PSCaa reacts with a nearby cysteine within the protein generating an azo bridge in situ. The resultant bridge is switchable by visible light and allows conformation and binding of CaM to be regulated by such light. This photoswitch should prove valuable in optobiology for its minimal interference, site flexibility, genetic encodability, and response to the more biocompatible visible light.

Effects of an Advanced Sleep Schedule and Morning Short Wavelength Light Exposure on Circadian Phase in Young Adults with Late Sleep Schedules

PubMed Central

Sharkey, Katherine M.; Carskadon, Mary A.; Figueiro, Mariana G.; Zhu, Yong; Rea, Mark S.

2011-01-01

Objective We examined the effects of an advanced sleep/wake schedule and morning short wavelength (blue) light in 25 adults (mean age±SD = 21.8±3 years; 13 women) with late sleep schedules and subclinical features of delayed sleep phase syndrome (DSPD). Methods After a baseline week, participants kept individualized, fixed, advanced 7.5-hour sleep schedules for 6 days. Participants were randomly assigned to groups to receive “blue” (470 nm, ~225 lux, n=12) or “dim” (< 1 lux, n=13) light for one hour after waking each day. Head-worn “Daysimeters” measured light exposure; actigraphs and sleep diaries confirmed schedule compliance. Salivary dim light melatonin onset (DLMO), self-reported sleep, and mood were examined with 2×2 ANOVA. Results After 6 days, both groups showed significant circadian phase advances, but morning blue-light was not associated with larger phase shifts than dim-light exposure. The average DLMO advances (mean±SD) were 1.5±1.1 hours in the dim light group and 1.4±0.7 hours in the blue light group. Conclusions Adherence to a fixed advanced sleep/wake schedule resulted in significant circadian phase shifts in young adults with subclinical DSPD with or without morning blue light exposure. Light/dark exposures associated with fixed early sleep schedules are sufficient to advance circadian phase in young adults. PMID:21704557

Advances in understanding the molecular basis of the first steps in color vision.

PubMed

Hofmann, Lukas; Palczewski, Krzysztof

2015-11-01

Serving as one of our primary environmental inputs, vision is the most sophisticated sensory system in humans. Here, we present recent findings derived from energetics, genetics and physiology that provide a more advanced understanding of color perception in mammals. Energetics of cis-trans isomerization of 11-cis-retinal accounts for color perception in the narrow region of the electromagnetic spectrum and how human eyes can absorb light in the near infrared (IR) range. Structural homology models of visual pigments reveal complex interactions of the protein moieties with the light sensitive chromophore 11-cis-retinal and that certain color blinding mutations impair secondary structural elements of these G protein-coupled receptors (GPCRs). Finally, we identify unsolved critical aspects of color tuning that require future investigation. Copyright © 2015. Published by Elsevier Ltd.

Bright light and thermoregulatory responses to exercise.

PubMed

Atkinson, G; Barr, D; Chester, N; Drust, B; Gregson, W; Reilly, T; Waterhouse, J

2008-03-01

The thermoregulatory responses to morning exercise after exposure to different schedules of bright light were examined. At 07:00 h, six males ran on two occasions in an environmental chamber (temperature = 31.4 +/- 1.0 degrees C, humidity = 66 +/- 6 %) for 40 min at 60 % of maximal oxygen uptake. Participants were exposed to bright light (10,000 lux) either between 22:00 - 23:00 h (BT (low)) or 06:00 - 07:00 h (BT (high)). Otherwise, participants remained in dim light (< 50 lux). It was hypothesized that BT (low) attenuates core temperature during morning exercise via the phase-delaying properties of evening bright light and by avoiding bright light in the morning. Evening bright light in BT (low) suppressed (p = 0.037) the increase in melatonin compared to dim light (1.1 +/- 11.4 vs. 15.2 +/- 19.7 pg x ml (-1)) and delayed (p = 0.034) the core temperature minimum by 1.46 +/- 1.24 h. Core temperature was 0.20 +/- 0.17 degrees C lower in BT (low) compared to BT (high) during the hour before exercise (p = 0.036), with evidence (p = 0.075) that this difference was maintained during exercise. Conversely, mean skin temperature was 1.0 +/- 1.7 degrees C higher during the first 10 min of exercise in BT (low) than in BT (high) (p = 0.030). There was evidence that the increase in perceived exertion was attenuated in BT (low) (p = 0.056). A chronobiologically-based light schedule can lower core temperature before and during morning exercise in hot conditions.

Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness.

PubMed

Corvalán, Claudia; Choe, Sunghwa

2017-01-01

Environmental conditions can affect stability of proteins at transcriptional or posttranscriptional levels to modulate their functions. Here we describe a method to observe changes in protein stability under different light conditions. In brief, Arabidopsis thaliana seedlings were maintained under various light regimes from continuous light to total darkness or transitions from light to dark, whereafter total protein was extracted from plants. Proteins were measured and resolved on sodium dodecyl sulfate-polyacrylamide gels and transferred to polyvinylidene difluoride membranes. Blots were incubated with the corresponding antibodies for the visualization of protein bands. The protocol described has been successfully applied in wild-type, different transgenic, and mutant background plants to study how light alone or in combination with other factors influences protein stability.

Not just signal shutoff: the protective role of arrestin-1 in rod cells.

PubMed

Sommer, Martha E; Hofmann, Klaus Peter; Heck, Martin

2014-01-01

The retinal rod cell is an exquisitely sensitive single-photon detector that primarily functions in dim light (e.g., moonlight). However, rod cells must routinely survive light intensities more than a billion times greater (e.g., bright daylight). One serious challenge to rod cell survival in daylight is the massive amount of all-trans-retinal that is released by Meta II, the light-activated form of the photoreceptor rhodopsin. All-trans-retinal is toxic, and its condensation products have been implicated in disease. Our recent work has developed the concept that rod arrestin (arrestin-1), which terminates Meta II signaling, has an additional role in protecting rod cells from the consequences of bright light by limiting free all-trans-retinal. In this chapter we will elaborate upon the molecular mechanisms by which arrestin-1 serves as both a single-photon response quencher as well as an instrument of rod cell survival in bright light. This discussion will take place within the framework of three distinct functional modules of vision: signal transduction, the retinoid cycle, and protein translocation.

Correlation of spatial intensity distribution of light reaching the retina and restoration of vision by optogenetic stimulation

NASA Astrophysics Data System (ADS)

Shivalingaiah, Shivaranjani; Gu, Ling; Mohanty, Samarendra K.

2011-03-01

Stimulation of retinal neuronal cells using optogenetics via use of chanelrhodopsin-2 (ChR2) and blue light has opened up a new direction for restoration of vision with respect to treatment of Retinitis pigmentosa (RP). In addition to delivery of ChR2 to specific retinal layer using genetic engineering, threshold level of blue light needs to be delivered onto the retina for generating action potential and successful behavioral outcome. We report measurement of intensity distribution of light reaching the retina of Retinitis pigmentosa (RP) mouse models and compared those results with theoretical simulations of light propagation in eye. The parameters for the stimulating source positioning in front of eye was determined for optimal light delivery to the retina. In contrast to earlier viral method based delivery of ChR2 onto retinal ganglion cells, in-vivo electroporation method was employed for retina-transfection of RP mice. The behavioral improvement in mice with Thy1-ChR2-YFP transfected retina, expressing ChR2 in retinal ganglion cells, was found to correlate with stimulation intensity.