Asparatic acid 221 is critical in the calcium-induced modulation of the enzymatic activity of human aminopeptidase A.

PubMed

Goto, Yoshikuni; Hattori, Akira; Mizutani, Shigehiko; Tsujimoto, Masafumi

2007-12-21

Aminopeptidase A (APA) plays an important role in the regulation of blood pressure by mediating angiotensin II degradation in the renin-angiotensin system. The Ca2+-induced modulation of enzymatic activity is the most characteristic feature of APA among the M1 family of aminopeptidases. In this study, we used site-directed mutagenesis for any residues responsible for the Ca2+ modulation of human APA. Alignment of sequences of the M1 family members led to the identification of Asp-221 as a significant residue of APA among the family members. Replacement of Asp-221 with Asn or Gln resulted in a loss of Ca2+ responsiveness toward synthetic substrates. These enzymes were also unresponsive to Ca2+ when peptide hormones, such as angiotensin II, cholecystokinin-8, neurokinin B, and kallidin, were employed as substrates. These results suggest that the negative charge of Asp-221 is essential for Ca2+ modulation of the enzymatic activity of APA and causes preferential cleavage of acidic amino acid at the N-terminal end of substrate peptides.

Device for use in a furnace exhaust stream for thermoelectric generation

DOEpatents

Polcyn, Adam D.

2013-06-11

A device for generating voltage or electrical current includes an inner elongated member mounted in an outer elongated member, and a plurality of thermoelectric modules mounted in the space between the inner and the outer members. The outer and/or inner elongated members each include a plurality of passages to move a temperature altering medium through the members so that the device can be used in high temperature environments, e.g. the exhaust system of an oxygen fired glass melting furnace. The modules are designed to include a biasing member and/or other arrangements to compensate for differences in thermal expansion between the first and the second members. In this manner, the modules remain in contact with the first and second members. The voltage generated by the modules can be used to power electrical loads.

The feasibility of e-learning as a quality improvement tool.

PubMed

Kobewka, Daniel; Backman, Chantal; Hendry, Paul; Hamstra, Stanley J; Suh, Kathryn N; Code, Catherine; Forster, Alan J

2014-10-01

Many quality problems exist in health care. We aim to investigate the feasibility and acceptability of using e-learning (defined as computer-based learning modules) to address gaps in quality of care. We performed a qualitative evaluation of participants in a pilot e-learning program. Physician members of six medical teaching units (MTUs) at a multi-site tertiary care teaching hospital were asked to complete two e-learning modules addressing hand hygiene practices and management of community-acquired pneumonia (CAP). An e-learning design team created online modules that were made available to members of the six MTUs for 4 weeks using a password secured website. Use of the modules was voluntary. Participants' perceptions of module content, mode of delivery, and suggestions for improvement were determined through focus groups. We then performed content analysis on the transcripts. We used system data to define patterns of module access. Out of 55 eligible users, 30 (55%) logged onto the system at least once. Residents (14/30, 47%) were less likely to use the system than medical students (9/14, 64%) and attending staff (7/11, 64%). Learners at all levels thought the modules were easy to use. Participants liked the knowledge-based material in the CAP module because it directly applied to their work. There were less favourable opinions of the hand hygiene module Generating e-learning modules targeted at gaps in quality of care is feasible and acceptable to learners. Future studies should assess whether these approaches lead to desired changes in behavior. © 2014 John Wiley & Sons, Ltd.

Evaluating students' perceptions of an interprofessional problem-based pilot learning project.

PubMed

Eccott, Lynda; Greig, Alison; Hall, Wendy; Lee, Michael; Newton, Christie; Wood, Victoria

2012-01-01

Interprofessional teams provide the promise of effective, comprehensive and reliable care. Interprofessional education (IPE) promotes students' knowledge and attitudes to support interprofessional teamwork, and problem-based learning formats enable students to gain valuable teamwork experience. To design, implement, and evaluate an interprofessional problem-based learning module in a large Canadian university focusing on the effects of this format on students' knowledge, attitudes, and perceptions. A pre-post mixed-methods research design was used, with a convenience sample of 24 students from medicine, pharmacy, nursing, physical therapy, and occupational therapy. Participants in the module were divided into 5 teams composed of one member from each discipline. Pre-tests were delivered just prior to module participation and post-tests directly followed. Students also participated in focus groups to provide feedback about module content, process, outcomes, and practical considerations. Students' attitudes toward interprofessional teamwork improved from baseline to post-intervention. Mean differences were significant using paired t-tests on confidence in professional role (p <0.001), communication (p = 0.02), understanding roles of others (p = 0.002), identification with the team (p = 0.002), comfort with members (p = 0.047), cooperation with team members (p = 0.004), team perceptions (p = 0.04), decision-making (p <0.001), team efficiency (p <0.001), minimal conflict (p = 0.04), and group contributions (p = 0.03). Focus group themes indicated students were satisfied with the module, perceived increased knowledge about roles and perspectives, greater confidence to collaborate, and increased motivation to engage in intra-curricular IPE. The timing of their exposure within their respective educational programs was identified as important.

Learning collaborative teamwork: an argument for incorporating the humanities.

PubMed

Hall, Pippa; Brajtman, Susan; Weaver, Lynda; Grassau, Pamela Anne; Varpio, Lara

2014-11-01

A holistic, collaborative interprofessional team approach, which includes patients and families as significant decision-making members, has been proposed to address the increasing burden being placed on the health-care system. This project hypothesized that learning activities related to the humanities during clinical placements could enhance interprofessional teamwork. Through an interprofessional team of faculty, clinical staff, students, and patient representatives, we developed and piloted the self-learning module, "interprofessional education for collaborative person-centred practice through the humanities". The module was designed to provide learners from different professions and educational levels with a clinical placement/residency experience that would enable them, through a lens of the humanities, to better understand interprofessional collaborative person-centred care without structured interprofessional placement activities. Learners reported the self-paced and self-directed module to be a satisfactory learning experience in all four areas of care at our institution, and certain attitudes and knowledge were significantly and positively affected. The module's evaluation resulted in a revised edition providing improved structure and instruction for students with no experience in self-directed learning. The module was recently adapted into an interactive bilingual (French and English) online e-learning module to facilitate its integration into the pre-licensure curriculum at colleges and universities.

The imitation game: Effects of social cues on 'imitation' are domain-general in nature.

PubMed

Marsh, Lauren E; Bird, Geoffrey; Catmur, Caroline

2016-10-01

Imitation has been hailed as 'social glue', facilitating rapport with others. Previous studies suggest that social cues modulate imitation but the mechanism of such modulation remains underspecified. Here we examine the locus, specificity, and neural basis of the social control of imitation. Social cues (group membership and eye gaze) were manipulated during an imitation task in which imitative and spatial compatibility could be measured independently. Participants were faster to perform compatible compared to incompatible movements in both spatial and imitative domains. However, only spatial compatibility was modulated by social cues: an interaction between group membership and eye gaze revealed more spatial compatibility for ingroup members with direct gaze and outgroup members with averted gaze. The fMRI data were consistent with this finding. Regions associated with the control of imitative responding (temporoparietal junction, inferior frontal gyrus) were more active during imitatively incompatible compared to imitatively compatible trials. However, this activity was not modulated by social cues. On the contrary, an interaction between group, gaze and spatial compatibility was found in the dorsolateral prefrontal cortex in a pattern consistent with reaction times. This region may be exerting control over the motor system to modulate response inhibition. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Analytical liquid test sample filtration apparatus

DOEpatents

Lohnes, B.C.; Turner, T.D.; Klingler, K.M.; Clark, M.L.

1996-01-09

A liquid sample filtration apparatus includes: (a) a module retaining filter elements; (b) a filter clamping and fluid injection apparatus positioned relative to the module to engage a filter element thereon, and includes a pair of first and second opposing engageable members to engage a filter element there between; (c) an inlet tube connected to an opposing engageable member; (d) an outlet tube connected to an opposing engageable member; (e) a motor to move the module relative to the filter clamping and injection apparatus to register filter elements on the module to the clamping and injection apparatus; and (f) a motor associated with the filter clamping and injection apparatus to move the opposing engageable members into substantial sealing fluid communication relative to a filter element on the module. An apparatus for engaging opposing ends of a filter element includes: (a) a member having a recess configured to engage one end of a filter element, including a first fluid passage communicating with the recess to pass fluid between the recess and externally of the member; and (b) a second member positioned in opposing juxtaposition relative to the other member, and having a projection sized and shaped to matingly fit within the other member recess, the second member projection including a second recess configured to engage the other end of the filter element, the second member including a second fluid passage communicating with the second recess to pass fluid between the second recess and externally of the second member. 8 figs.

Analytical liquid test sample filtration apparatus

DOEpatents

Lohnes, Brent C.; Turner, Terry D.; Klingler, Kerry M.; Clark, Michael L.

1996-01-01

A liquid sample filtration apparatus includes: a) a module retaining filter elements; b) a filter clamping and fluid injection apparatus positioned relative to the module to engage a filter element thereon, and includes a pair of first and second opposing engageable members to sealing engage a filter element therebetween; c) an inlet tube connected to an opposing engageable member; d) an outlet tube connected to an opposing engageable member; e) a motor to move the module relative to the filter clamping and injection apparatus to register filter elements on the module to the clamping and injection apparatus; and f) a motor associated with the filter clamping and injection apparatus to move the opposing engageable members into substantial sealing fluid communication relative to a filter element on the module. An apparatus for engaging opposing ends of a filter element includes: a) a member having a recess configured to engage one end of a filter element, including a first fluid passage communicating with the recess to pass fluid between the recess and externally of the member; and b) a second member positioned in opposing juxtaposition relative to the other member, and having a projection sized and shaped to matingly fit within the other member recess, the second member projection including a second recess configured to engage the other end of the filter element, the second member including a second fluid passage communicating with the second recess to pass fluid between the second recess and externally of the second member.

Modular heat exchanger

DOEpatents

Giardina, A.R.

1981-03-03

A shell and tube heat exchanger is described having a plurality of individually removable tube bundle modules. A lattice of structural steel forming rectangular openings therein is placed at each end of a cylindrical shell. Longitudinal structural members are placed in the shell between corners of the rectangular openings situated on opposite ends of the shell. Intermediate support members interconnect the longitudinal supports so as to increase the longitudinal supports rigidity. Rectangular parallelepiped tube bundle modules occupy the space defined by the longitudinal supports and end supports and each include a rectangular tube sheet situated on each end of a plurality of tubes extending there through, a plurality of rectangular tube supports located between the tube sheets, and a tube bundle module stiffening structure disposed about the bundle's periphery and being attached to the tube sheets and tube supports. The corners of each tube bundle module have longitudinal framework members which are mateable with and supported by the longitudinal support members. Intermediate support members constitute several lattices, each of which is situated in a plane between the end support members. The intermediate support members constituting the several lattices extend horizontally and vertically between longitudinal supports of adjacent tube module voids. An alternative embodiment for intermediate support members constitute a series of structural plates situated at the corners of the module voids and having recesses therein for receiving the respective longitudinal support members adjacent thereto, protrusions separating the recesses, and a plurality of struts situated between protrusions of adjacent structural plates. 12 figs.

Signaling threshold regulation by the Ras effector IMP.

PubMed

Matheny, Sharon A; White, Michael A

2009-04-24

The Ras effector and E3 ligase family member IMP (impedes mitogenic signal propagation) acts as a steady-state resistor within the Raf-MEK-ERK kinase module. IMP concentrations are directly regulated by Ras, through induction of autoubiquitination, to permit productive Raf-MEK complex assembly. Inhibition of Raf-MEK pathway activation by IMP occurs through the inactivation of KSR, a scaffold/adapter protein that couples activated Raf to its substrate MEK1. The capacity of IMP to inhibit signal propagation through Raf to MEK is, in part, a consequence of disrupting KSR1 homo-oligomerization and c-Raf-B-Raf hetero-oligomerization. These observations suggest that IMP functions as a threshold modulator, controlling sensitivity of the cascade to stimulus by directly limiting the assembly of functional KSR1-dependent Raf-MEK complexes.

Categorization Is Modulated by Transcranial Direct Current Stimulation over Left Prefrontal Cortex

ERIC Educational Resources Information Center

Lupyan, Gary; Mirman, Daniel; Hamilton, Roy; Thompson-Schill, Sharon L.

2012-01-01

Humans have an unparalleled ability to represent objects as members of multiple categories. A given object, such as a pillow may be--depending on current task demands--represented as an instance of something that is soft, as something that contains feathers, as something that is found in bedrooms, or something that is larger than a toaster. This…

Illuminance and luminance distributions of a prototype ambient illumination system for Space Station Freedom

NASA Technical Reports Server (NTRS)

Mullican, R. C.; Hayes, B. C.

1991-01-01

Preliminary results of research conducted in the late 1970's indicate that perceptual qualities of an enclosure can be influenced by the distribution of illumination within the enclosure. Subjective impressions such as spaciousness, perceptual clarity, and relaxation or tenseness, among others, appear to be related to different combinations of surface luminance. A prototype indirect ambient illumination system was developed which will allow crew members to alter surface luminance distributions within an enclosed module, thus modifying perceptual cues to match crew preferences. A traditional lensed direct lighting system was compared to the prototype utilizing the full-scale mockup of Space Station Freedom developed by Marshall Space Flight Center. The direct lensed system was installed in the habitation module with the indirect prototype deployed in the U.S. laboratory module. Analysis centered on the illuminance and luminance distributions resultant from these systems and the implications of various luminaire spacing options. All test configurations were evaluated for compliance with NASA Standard 3000, Man-System Integration Standards.

Structural basis for alcohol modulation of a pentameric ligand-gated ion channel

PubMed Central

Howard, Rebecca J.; Murail, Samuel; Ondricek, Kathryn E.; Corringer, Pierre-Jean; Lindahl, Erik; Trudell, James R.; Harris, R. Adron

2011-01-01

Despite its long history of use and abuse in human culture, the molecular basis for alcohol action in the brain is poorly understood. The recent determination of the atomic-scale structure of GLIC, a prokaryotic member of the pentameric ligand-gated ion channel (pLGIC) family, provides a unique opportunity to characterize the structural basis for modulation of these channels, many of which are alcohol targets in brain. We observed that GLIC recapitulates bimodal modulation by n-alcohols, similar to some eukaryotic pLGICs: methanol and ethanol weakly potentiated proton-activated currents in GLIC, whereas n-alcohols larger than ethanol inhibited them. Mapping of residues important to alcohol modulation of ionotropic receptors for glycine, γ-aminobutyric acid, and acetylcholine onto GLIC revealed their proximity to transmembrane cavities that may accommodate one or more alcohol molecules. Site-directed mutations in the pore-lining M2 helix allowed the identification of four residues that influence alcohol potentiation, with the direction of their effects reflecting α-helical structure. At one of the potentiation-enhancing residues, decreased side chain volume converted GLIC into a highly ethanol-sensitive channel, comparable to its eukaryotic relatives. Covalent labeling of M2 positions with an alcohol analog, a methanethiosulfonate reagent, further implicated residues at the extracellular end of the helix in alcohol binding. Molecular dynamics simulations elucidated the structural consequences of a potentiation-enhancing mutation and suggested a structural mechanism for alcohol potentiation via interaction with a transmembrane cavity previously termed the “linking tunnel.” These results provide a unique structural model for independent potentiating and inhibitory interactions of n-alcohols with a pLGIC family member. PMID:21730162

Modular heat exchanger

DOEpatents

Giardina, Angelo R. [Marple Township, Delaware County, PA

1981-03-03

A shell and tube heat exchanger having a plurality of individually removable tube bundle modules. A lattice of structural steel forming rectangular openings therein is placed at each end of a cylindrical shell. Longitudinal structural members are placed in the shell between corners of the rectangular openings situated on opposite ends of the shell. Intermediate support members interconnect the longitudinal supports so as to increase the longitudinal supports rigidity. Rectangular parallelpiped tube bundle moldules occupy the space defined by the longitudinal supports and end supports and each include a rectangular tube sheet situated on each end of a plurality of tubes extending therethrough, a plurality of rectangular tube supports located between the tube sheets, and a tube bundle module stiffening structure disposed about the bundle's periphery and being attached to the tube sheets and tube supports. The corners of each tube bundle module have longitudinal framework members which are mateable with and supported by the longitudinal support members. Intermediate support members constitute several lattice, each of which is situate d in a plane between the end support members. The intermediate support members constituting the several lattice extend horizontally and vertically between longitudinal supports of adjacent tube module voids. An alternative embodiment for intermediate support members constitute a series of structural plates situated at the corners of the module voids and having recesses therein for receiving the respective longitudinal support members adjacent thereto, protrusions separating the recesses, and a plurality of struts situated between protrusions of adjacent structural plates.

KSC-08pd1457

NASA Image and Video Library

2008-05-28

CAPE CANAVERAL, Fla. -- Replacement parts for the Zvezda service module toilet on the International Space Station are inspected following their arrival at Kennedy Space Center. The toilet malfunctioned last week and was initially repaired by replacing a microprocessor valve. After the station crew members experienced additional difficulties with the toilet, they were directed to use Soyuz toilet facilities at first and are using the main toilet again after rigging a urine bypass. The spare toilet parts have been added to space shuttle Discovery’s manifest for delivery to the station on the STS-124 mission. On the 14-day mission, Discovery and its crew will deliver the Japan Aerospace Exploration Agency's Japanese Experiment Module – Pressurized Module and the Japanese Remote Manipulator System. Launch is scheduled for 5:02 p.m. EDT May 31. Photo credit: NASA/Kim Shiflett

KSC-08pd1458

NASA Image and Video Library

2008-05-28

CAPE CANAVERAL, Fla. -- Replacement parts for the Zvezda service module toilet on the International Space Station are inspected following their arrival at Kennedy Space Center. The toilet malfunctioned last week and was initially repaired by replacing a microprocessor valve. After the station crew members experienced additional difficulties with the toilet, they were directed to use Soyuz toilet facilities at first and are using the main toilet again after rigging a urine bypass. The spare toilet parts have been added to space shuttle Discovery’s manifest for delivery to the station on the STS-124 mission. On the 14-day mission, Discovery and its crew will deliver the Japan Aerospace Exploration Agency's Japanese Experiment Module – Pressurized Module and the Japanese Remote Manipulator System. Launch is scheduled for 5:02 p.m. EDT May 31. Photo credit: NASA/Kim Shiflett

Pharmacological Modulation of Diacylglycerol-Sensitive TRPC3/6/7 Channels

PubMed Central

Harteneck, Christian; Gollasch, Maik

2011-01-01

Members of the classic type of transient receptor potential channels (TRPC) represent important molecules involved in hormonal signal transduction. TRPC3/6/7 channels are of particular interest as they are components of phospholipase C driven signalling pathways. Upon receptor-activation, G-protein-mediated stimulation of phospholipase C results in breakdown of phosphatidylinositides leading to increased intracellular diacylglycerol and inositol-trisphosphate levels. Diacylglycerol activates protein kinase C, but more interestingly diacylglycerol directly activates TRPC2/3/6/7 channels. Molecular cloning, expression and characterization of TRP channels enabled reassignment of traditional inhibitors of receptor-dependent calcium entry such as SKF-96365 and 2-APB as blockers of TRPC3/6/7 and several members of non-classic TRP channels. Furthermore, several enzyme inhibitors have also been identified as TRP channel blockers, such as ACA, a phospholipase A2 inhibitor, and W-7, a calmodulin antagonist. Finally, the naturally occurring secondary plant compound hyperforin has been identified as TRPC6-selective drug, providing an exciting proof of concept that it is possible to generate TRPC-selective channel modulators. The description of Pyr3 as the first TRPC3-selective inhibitor shows that not only nature but also man is able to generate TRP-selective modulators. The review sheds lights on the current knowledge and historical development of pharmacological modulators of TRPC3/6/7. Our analysis indicates that Pyr3 and hyperforin provide promising core structures for the development of new, selective and more potent modulators of TRPC3/6/7 activity. PMID:20932261

SPACEHAB: A giant step in the commercial development of space

NASA Astrophysics Data System (ADS)

Shepard, James E.

SPACEHAB is a privately developed and operated system offering customers a crew-tended microgravity environment for experimentation and product development. The first SPACEHAB flight module was delivered to the SPACEHAB Payload Processing Facility (SPPF) in Florida and 22 experiments are being integrated for an April 1993 mission. SPACEHAB modules are flown in the forward quarter-bay of the NASA Orbiter and are supported by two crew members. The paylaod accommodations include up to 61 experiment lockers, double and single racks and standard mounting plates for mounting unique payload containers directly to the module structure. Experiments designed for the Orbiter mid-deck, Spacelab or Space Station Freedom can be flown in SPACEHAB. The 24-month integration cycle is currently the shortest for any crew-tended carrier; a goal of 18 months is being actively pursued.

KSC-08pd1462

NASA Image and Video Library

2008-05-29

CAPE CANAVERAL, Fla. -- At Launch Pad 39A at Kennedy Space Center, replacement parts for the Zvezda service module toilet on the International Space Station are loaded aboard space shuttle Discovery. The toilet malfunctioned last week and was initially repaired by replacing a microprocessor valve. After the station crew members experienced additional difficulties with the toilet, they were directed to use Soyuz toilet facilities at first and are using the main toilet again after rigging a urine bypass. The spare toilet parts have been added to Discovery’s manifest for delivery to the station on the STS-124 mission. On the 14-day mission, Discovery and its crew will deliver the Japan Aerospace Exploration Agency's Japanese Experiment Module – Pressurized Module and the Japanese Remote Manipulator System. Launch is scheduled for 5:02 p.m. EDT May 31. Photo credit: NASA/Dimitri Gerondidakis

KSC-08pd1459

NASA Image and Video Library

2008-05-28

CAPE CANAVERAL, Fla. -- A replacement part for the Zvezda service module toilet on the International Space Station is inspected following its arrival at Kennedy Space Center. The toilet malfunctioned last week and was initially repaired by replacing a microprocessor valve. After the station crew members experienced additional difficulties with the toilet, they were directed to use Soyuz toilet facilities at first and are using the main toilet again after rigging a urine bypass. The spare toilet parts have been added to space shuttle Discovery’s manifest for delivery to the station on the STS-124 mission. On the 14-day mission, Discovery and its crew will deliver the Japan Aerospace Exploration Agency's Japanese Experiment Module – Pressurized Module and the Japanese Remote Manipulator System. Launch is scheduled for 5:02 p.m. EDT May 31. Photo credit: NASA/Kim Shiflett

KSC-08pd1464

NASA Image and Video Library

2008-05-29

CAPE CANAVERAL, Fla. -- At Launch Pad 39A at Kennedy Space Center, technicians load replacement parts for the Zvezda service module toilet on the International Space Station aboard space shuttle Discovery. The toilet malfunctioned last week and was initially repaired by replacing a microprocessor valve. After the station crew members experienced additional difficulties with the toilet, they were directed to use Soyuz toilet facilities at first and are using the main toilet again after rigging a urine bypass. The spare toilet parts have been added to Discovery’s manifest for delivery to the station on the STS-124 mission. On the 14-day mission, Discovery and its crew will deliver the Japan Aerospace Exploration Agency's Japanese Experiment Module – Pressurized Module and the Japanese Remote Manipulator System. Launch is scheduled for 5:02 p.m. EDT May 31. Photo credit: NASA/Dimitri Gerondidakis

KSC-08pd1460

NASA Image and Video Library

2008-05-28

CAPE CANAVERAL, Fla. -- A technician inspects a replacement part for the Zvezda service module toilet on the International Space Station following its arrival at Kennedy Space Center. The toilet malfunctioned last week and was initially repaired by replacing a microprocessor valve. After the station crew members experienced additional difficulties with the toilet, they were directed to use Soyuz toilet facilities at first and are using the main toilet again after rigging a urine bypass. The spare toilet parts have been added to space shuttle Discovery’s manifest for delivery to the station on the STS-124 mission. On the 14-day mission, Discovery and its crew will deliver the Japan Aerospace Exploration Agency's Japanese Experiment Module – Pressurized Module and the Japanese Remote Manipulator System. Launch is scheduled for 5:02 p.m. EDT May 31. Photo credit: NASA/Kim Shiflett

KSC-08pd1463

NASA Image and Video Library

2008-05-29

CAPE CANAVERAL, Fla. -- At Launch Pad 39A at Kennedy Space Center, technicians load replacement parts for the Zvezda service module toilet on the International Space Station aboard space shuttle Discovery. The toilet malfunctioned last week and was initially repaired by replacing a microprocessor valve. After the station crew members experienced additional difficulties with the toilet, they were directed to use Soyuz toilet facilities at first and are using the main toilet again after rigging a urine bypass. The spare toilet parts have been added to Discovery’s manifest for delivery to the station on the STS-124 mission. On the 14-day mission, Discovery and its crew will deliver the Japan Aerospace Exploration Agency's Japanese Experiment Module – Pressurized Module and the Japanese Remote Manipulator System. Launch is scheduled for 5:02 p.m. EDT May 31. Photo credit: NASA/Dimitri Gerondidakis

KSC-08pd1465

NASA Image and Video Library

2008-05-29

CAPE CANAVERAL, Fla. -- At Launch Pad 39A at Kennedy Space Center, a technician loads replacement parts for the Zvezda service module toilet on the International Space Station aboard space shuttle Discovery. The toilet malfunctioned last week and was initially repaired by replacing a microprocessor valve. After the station crew members experienced additional difficulties with the toilet, they were directed to use Soyuz toilet facilities at first and are using the main toilet again after rigging a urine bypass. The spare toilet parts have been added to Discovery’s manifest for delivery to the station on the STS-124 mission. On the 14-day mission, Discovery and its crew will deliver the Japan Aerospace Exploration Agency's Japanese Experiment Module – Pressurized Module and the Japanese Remote Manipulator System. Launch is scheduled for 5:02 p.m. EDT May 31. Photo credit: NASA/Dimitri Gerondidakis

KSC-08pd1461

NASA Image and Video Library

2008-05-28

CAPE CANAVERAL, Fla. -- A technician inspects a replacement part for the Zvezda service module toilet on the International Space Station following its arrival at Kennedy Space Center. The toilet malfunctioned last week and was initially repaired by replacing a microprocessor valve. After the station crew members experienced additional difficulties with the toilet, they were directed to use Soyuz toilet facilities at first and are using the main toilet again after rigging a urine bypass. The spare toilet parts have been added to space shuttle Discovery’s manifest for delivery to the station on the STS-124 mission. On the 14-day mission, Discovery and its crew will deliver the Japan Aerospace Exploration Agency's Japanese Experiment Module – Pressurized Module and the Japanese Remote Manipulator System. Launch is scheduled for 5:02 p.m. EDT May 31. Photo credit: NASA/Kim Shiflett

Supported PV module assembly

DOEpatents

Mascolo, Gianluigi; Taggart, David F.; Botkin, Jonathan D.; Edgett, Christopher S.

2013-10-15

A supported PV assembly may include a PV module comprising a PV panel and PV module supports including module supports having a support surface supporting the module, a module registration member engaging the PV module to properly position the PV module on the module support, and a mounting element. In some embodiments the PV module registration members engage only the external surfaces of the PV modules at the corners. In some embodiments the assembly includes a wind deflector with ballast secured to a least one of the PV module supports and the wind deflector. An array of the assemblies can be secured to one another at their corners to prevent horizontal separation of the adjacent corners while permitting the PV modules to flex relative to one another so to permit the array of PV modules to follow a contour of the support surface.

Photovoltaic module with removable wind deflector

DOEpatents

Botkin, Jonathan [El Cerrito, CA; Graves, Simon [Berkeley, CA; Danning, Matt [Oakland, CA; Culligan, Matthew [Berkeley, CA

2012-08-07

A photovoltaic (PV) module assembly including a PV module, a deflector, and a clip. The PV module includes a PV device and a frame. A PV laminate is assembled to the frame, and the frame includes a support arm forming a seat. The deflector defines a front face and a rear face, with the clip extending from either the trailing frame member or the rear face of the deflector. In a mounted state, the deflector is nested within the seat and is releasably mounted to the trailing frame member via the clip. In some embodiments, the support arm forms a second seat, with the PV module assembly providing a second mounted state in which the deflector is in a differing orientation/slope, nested within the second seat and releasably mounted to the trailing frame member via the clip.

Photovoltaic module with removable wind deflector

DOEpatents

Botkin, Jonathan; Graves, Simon; Danning, Matt; Culligan, Matthew

2014-02-18

A photovoltaic (PV) module assembly including a PV module, a deflector, and a clip. The PV module includes a PV device and a frame. A PV laminate is assembled to the frame, and the frame includes a support arm forming a seat. The deflector defines a front face and a rear face, with the clip extending from either the trailing frame member or the rear face of the deflector. In a mounted state, the deflector is nested within the seat and is releasably mounted to the trailing frame member via the clip. In some embodiments, the support arm forms a second seat, with the PV module assembly providing a second mounted state in which the deflector is in a differing orientation/slope, nested within the second seat and releasably mounted to the trailing frame member via the clip.

Photovoltaic module with removable wind deflector

DOEpatents

Botkin, Jonathan; Graves, Simon; Danning, Matt; Culligan, Matthew

2013-05-28

A photovoltaic (PV) module assembly including a PV module, a deflector, and a clip. The PV module includes a PV device and a frame. A PV laminate is assembled to the frame, and the frame includes a support arm forming a seat. The deflector defines a front face and a rear face, with the clip extending from either the trailing frame member or the rear face of the deflector. In a mounted state, the deflector is nested within the seat and is releasably mounted to the trailing frame member via the clip. In some embodiments, the support arm forms a second seat, with the PV module assembly providing a second mounted state in which the deflector is in a differing orientation/slope, nested within the second seat and releasably mounted to the trailing frame member via the clip.

Histone deacetylase 3 indirectly modulates tubulin acetylation.

PubMed

Bacon, Travis; Seiler, Caroline; Wolny, Marcin; Hughes, Ruth; Watson, Peter; Schwabe, John; Grigg, Ronald; Peckham, Michelle

2015-12-15

Histone deacetylase 3 (HDAC3), a member of the Class I subfamily of HDACs, is found in both the nucleus and the cytoplasm. Its roles in the nucleus have been well characterized, but its cytoplasmic roles are still not elucidated fully. We found that blocking HDAC3 activity using MI192, a compound specific for HDAC3, modulated tubulin acetylation in the human prostate cancer cell line PC3. A brief 1 h treatment of PC3 cells with MI192 significantly increased levels of tubulin acetylation and ablated the dynamic behaviour of microtubules in live cells. siRNA-mediated knockdown (KD) of HDAC3 in PC3 cells, significantly increased levels of tubulin acetylation, and overexpression reduced it. However, the active HDAC3-silencing mediator of retinoic and thyroid receptors (SMRT)-deacetylase-activating domain (DAD) complex did not directly deacetylate tubulin in vitro. These data suggest that HDAC3 indirectly modulates tubulin acetylation. © 2015 Authors.

Optimizing cholinergic tone through lynx modulators of nicotinic receptors: implications for plasticity and nicotine addiction.

PubMed

Miwa, Julie M; Lester, Henry A; Walz, Andreas

2012-08-01

The cholinergic system underlies both adaptive (learning and memory) and nonadaptive (addiction and dependency) behavioral changes through its ability to shape and regulate plasticity. Protein modulators such as lynx family members can fine tune the activity of the cholinergic system and contribute to the graded response of the cholinergic system, stabilizing neural circuitry through direct interaction with nicotinic receptors. Release of this molecular brake can unmask cholinergic-dependent mechanisms in the brain. Lynx proteins have the potential to provide top-down control over plasticity mechanisms, including addictive propensity. If this is indeed the case, then, what regulates the regulator? Transcriptional changes of lynx genes in response to pharmacological, physiological, and pathological alterations are explored in this review.

Racial Bias in Neural Response for Pain Is Modulated by Minimal Group

PubMed Central

Shen, Fengtao; Hu, Yang; Fan, Mingxia; Wang, Huimin; Wang, Zhaoxin

2018-01-01

Whether empathic racial bias could be modulated is a subject of intense interest. The present study was carried out to explore whether empathic racial bias for pain is modulated by minimal group. Chinese/Western faces with neutral expressions receiving painful (needle penetration) or non-painful (Q-tip touch) stimulation were presented. Participants were asked to rate the pain intensity felt by Chinese/Western models of ingroup/outgroup members. Their implicit racial bias were also measured. Two lines of evidence indicated that the anterior cingulate cortex (ACC) was modulated by racial bias: (1) Chinese models elicited stronger activity than Western did in the ACC, and (2) activity in the ACC was modulated by implicit racial bias. Whereas the right anterior insula (rAI) were modulated by ingroup bias, in which ingroup member elicited stronger activity than outgroup member did. Furthermore, activity in the ACC was modulated by activity of rAI (i.e., ingroup bias) in the pain condition, while activity in the rAI was modulated by activity of ACC (i.e., racial bias) in the nopain condition. Our results provide evidence that there are different neural correlates for racial bias and ingroup bias, and neural racial bias for pain can be modulated by minimal group. PMID:29379429

Racial Bias in Neural Response for Pain Is Modulated by Minimal Group.

PubMed

Shen, Fengtao; Hu, Yang; Fan, Mingxia; Wang, Huimin; Wang, Zhaoxin

2017-01-01

Whether empathic racial bias could be modulated is a subject of intense interest. The present study was carried out to explore whether empathic racial bias for pain is modulated by minimal group. Chinese/Western faces with neutral expressions receiving painful (needle penetration) or non-painful (Q-tip touch) stimulation were presented. Participants were asked to rate the pain intensity felt by Chinese/Western models of ingroup/outgroup members. Their implicit racial bias were also measured. Two lines of evidence indicated that the anterior cingulate cortex (ACC) was modulated by racial bias: (1) Chinese models elicited stronger activity than Western did in the ACC, and (2) activity in the ACC was modulated by implicit racial bias. Whereas the right anterior insula (rAI) were modulated by ingroup bias, in which ingroup member elicited stronger activity than outgroup member did. Furthermore, activity in the ACC was modulated by activity of rAI (i.e., ingroup bias) in the pain condition, while activity in the rAI was modulated by activity of ACC (i.e., racial bias) in the nopain condition. Our results provide evidence that there are different neural correlates for racial bias and ingroup bias, and neural racial bias for pain can be modulated by minimal group.

Two members of Apollo 8 crew suited up for centrifuge training

NASA Technical Reports Server (NTRS)

1968-01-01

Two members of the Apollo 8 prime crew stand beside the gondola in bldg 29 after suiting up for centrifuge training in the Manned Spacecraft Center's (MSC) Flight Acceleration Facility. They are Astronauts William A. Anders (left), lunar module pilot; and James A. Lovell Jr., command module pilot.

Menin regulates Inhbb expression through an Akt/Ezh2-mediated H3K27 histone modification.

PubMed

Gherardi, Samuele; Ripoche, Doriane; Mikaelian, Ivan; Chanal, Marie; Teinturier, Romain; Goehrig, Delphine; Cordier-Bussat, Martine; Zhang, Chang X; Hennino, Ana; Bertolino, Philippe

2017-04-01

Although Men1 is a well-known tumour suppressor gene, little is known about the functions of Menin, the protein it encodes for. Since few years, numerous publications support a major role of Menin in the control of epigenetics gene regulation. While Menin interaction with MLL complex favours transcriptional activation of target genes through H3K4me3 marks, Menin also represses gene expression via mechanisms involving the Polycomb repressing complex (PRC). Interestingly, Ezh2, the PRC-methyltransferase that catalyses H3K27me3 repressive marks and Menin have been shown to co-occupy a large number of promoters. However, lack of binding between Menin and Ezh2 suggests that another member of the PRC complex is mediating this indirect interaction. Having found that ActivinB - a TGFβ superfamily member encoded by the Inhbb gene - is upregulated in insulinoma tumours caused by Men1 invalidation, we hypothesize that Menin could directly participate in the epigenetic-repression of Inhbb gene expression. Using Animal model and cell lines, we report that loss of Menin is directly associated with ActivinB-induced expression both in vivo and in vitro. Our work further reveals that ActivinB expression is mediated through a direct modulation of H3K27me3 marks on the Inhbb locus in Menin-KO cell lines. More importantly, we show that Menin binds on the promoter of Inhbb gene where it favours the recruitment of Ezh2 via an indirect mechanism involving Akt-phosphorylation. Our data suggests therefore that Menin could take an important part to the Ezh2-epigenetic repressive landscape in many cells and tissues through its capacity to modulate Akt phosphorylation. Copyright © 2017 Elsevier B.V. All rights reserved.

STS-98 Onboard Photograph-U.S. Laboratory, Destiny

NASA Technical Reports Server (NTRS)

2001-01-01

With its new U.S. Laboratory, Destiny, contrasted over a blue and white Earth, the International Space Station (ISS) was photographed by one of the STS-98 crew members aboard the Space Shuttle Atlantis following separation of the Shuttle and Station. The Laboratory is shown at the lower right of the Station. The American-made Destiny module is the cornerstone for space-based research aboard the orbiting platform and the centerpiece of the ISS, where unprecedented science experiments will be performed in the near-zero gravity of space. Destiny will also serve as the command and control center for the ISS. The aluminum module is 8.5- meters (28-feet) long and 4.3-meters (14-feet) in diameter. The laboratory consists of three cylindrical sections and two endcones with hatches that will be mated to other station components. A 50.9-centimeter (20-inch-) diameter window is located on one side of the center module segment. This pressurized module is designed to accommodate pressurized payloads. It has a capacity of 24 rack locations. Payload racks will occupy 15 locations especially designed to support experiments. The Destiny module was built by the Boeing Company under the direction of the Marshall Space Flight Center.

International Space Station (ISS)

NASA Image and Video Library

2001-02-16

The International Space Station (ISS), with its newly attached U.S. Laboratory, Destiny, was photographed by a crew member aboard the Space Shuttle Orbiter Atlantis during a fly-around inspection after Atlantis separated from the Space Station. The Laboratory is shown in the foreground of this photograph. The American-made Destiny module is the cornerstone for space-based research aboard the orbiting platform and the centerpiece of the International Space Station (ISS), where unprecedented science experiments will be performed in the near-zero gravity of space. Destiny will also serve as the command and control center for the ISS. The aluminum module is 8.5-meters (28-feet) long and 4.3-meters (14-feet) in diameter. The laboratory consists of three cylindrical sections and two endcones with hatches that will be mated to other station components. A 50.9-centimeter (20-inch-) diameter window is located on one side of the center module segment. This pressurized module is designed to accommodate pressurized payloads. It has a capacity of 24 rack locations. Payload racks will occupy 15 locations especially designed to support experiments. The Destiny module was built by the Boeing Company under the direction of the Marshall Space Flight Center.

International Space Station (ISS)

NASA Image and Video Library

2001-02-16

With its new U.S. Laboratory, Destiny, contrasted over a blue and white Earth, the International Space Station (ISS) was photographed by one of the STS-98 crew members aboard the Space Shuttle Atlantis following separation of the Shuttle and Station. The Laboratory is shown at the lower right of the Station. The American-made Destiny module is the cornerstone for space-based research aboard the orbiting platform and the centerpiece of the ISS, where unprecedented science experiments will be performed in the near-zero gravity of space. Destiny will also serve as the command and control center for the ISS. The aluminum module is 8.5- meters (28-feet) long and 4.3-meters (14-feet) in diameter. The laboratory consists of three cylindrical sections and two endcones with hatches that will be mated to other station components. A 50.9-centimeter (20-inch-) diameter window is located on one side of the center module segment. This pressurized module is designed to accommodate pressurized payloads. It has a capacity of 24 rack locations. Payload racks will occupy 15 locations especially designed to support experiments. The Destiny module was built by the Boeing Company under the direction of the Marshall Space Flight Center.

Process of making solar cell module

DOEpatents

Packer, M.; Coyle, P.J.

1981-03-09

A process is presented for the manufacture of solar cell modules. A solution comprising a highly plasticized polyvinyl butyral is applied to a solar cell array. The coated array is dried and sandwiched between at last two sheets of polyvinyl butyral and at least two sheets of a rigid transparent member. The sandwich is laminated by the application of heat and pressure to cause fusion and bonding of the solar cell array with the rigid transparent members to produce a solar cell module.

View of the STS-88 crew in the Node 1/Unity module

NASA Image and Video Library

1998-12-10

STS088-322-021 (4-15 DECEMBER 1998) --- Astronaut Robert D. Cabana (left), mission commander, and cosmonaut Sergei K. Krikalev, mission specialist representing the Russian Space Agency (RSA), plan their approach to tasks in the U.S.-built Unity module. All six STS-88 crew members were involved in tasks to ready Unity and the now-connected Russian-built FGB module, also called Zarya, for their International Space Station (ISS) roles. Krikalev has been named as a member of the first ISS crew.

STS-112 Flight Day 3 Highlights

NASA Technical Reports Server (NTRS)

2002-01-01

During the third flight day of STS-112 (Commander Jeff Ashby, Pilot Pam Melroy and Mission Specialists Sandy Magnus, Piers Sellers, David Wolf and Fyodor Yurchikhin), the Space Shuttle Atlantis begins its final approach to the International Space Station (ISS) with which it will dock. The Chinese mainland is seen, at night, at a height of 242 statute miles. In one section of video from a camera onboard the ISS, Atlantis can be seen to be almost directly below the station, at a distance of several hundred feet. The orbiter's docking system is shown, as it is slowly guided by Ashby towards the forward docking port on the ISS's Destiny Laboratory Module and its forward docking port. Above the docking port, the S0 truss structure can be seen, to which the S1 truss structure in Atlantis' payload bay will be attached during this mission. Also seen are the Unity airlock and other modules. Following the completion of docking, in which an excellent shot of the docking system in hard dock is visible, the hatches between the two crafts are opened and the members of Atlantis are greeted by the very excited members of Expedition 5, who have been aboard the ISS for several months.

The prevalence and costs of obesity in the EU.

PubMed

Fry, James; Finley, Willa

2005-08-01

The present paper reports on results obtained as part of the economics module of the LIPGENE project. It reviews recent trends in obesity in the fifteen member states of the EU (the member states before enlargement in 2004) and concludes that in 2002 for both adult men and women at least half the fifteen member states of the EU had obesity levels of >20%. In the same year the total direct and indirect annual costs of obesity in the fifteen member states of the EU were 32,800 euro x 10(6). The provision of 'healthy' foods with an adapted lipid profile provides one means of mitigating the impact of obesity. However, there are extra costs associated with the supply of such foods, via additional costs of food ingredients, identity preservation costs for premium-value food products and the penalties caused by the inability to exploit economies of scale. The limited scale of the markets for healthy foods is caused by their high selling prices. Also presented is an analysis of the costs of subsidising, throughout the EU, ingredients that improve the fatty acid profile of beef, eggs, poultry meat and low-fat spreads, so that healthier versions of these products sell at the same price as traditional alternatives. The total costs of such subsidies for these four food products would have been 10 euro x 10(9)/year in 2002, which was 30% of the direct and indirect costs of obesity.

Histone deacetylase 3 indirectly modulates tubulin acetylation

PubMed Central

Bacon, Travis; Seiler, Caroline; Wolny, Marcin; Hughes, Ruth; Watson, Peter; Schwabe, John; Grigg, Ronald; Peckham, Michelle

2015-01-01

Histone deacetylase 3 (HDAC3), a member of the Class I subfamily of HDACs, is found in both the nucleus and the cytoplasm. Its roles in the nucleus have been well characterized, but its cytoplasmic roles are still not elucidated fully. We found that blocking HDAC3 activity using MI192, a compound specific for HDAC3, modulated tubulin acetylation in the human prostate cancer cell line PC3. A brief 1 h treatment of PC3 cells with MI192 significantly increased levels of tubulin acetylation and ablated the dynamic behaviour of microtubules in live cells. siRNA-mediated knockdown (KD) of HDAC3 in PC3 cells, significantly increased levels of tubulin acetylation, and overexpression reduced it. However, the active HDAC3–silencing mediator of retinoic and thyroid receptors (SMRT)–deacetylase-activating domain (DAD) complex did not directly deacetylate tubulin in vitro. These data suggest that HDAC3 indirectly modulates tubulin acetylation. PMID:26450925

Dysregulated fibroblast growth factor (FGF) signaling in neurological and psychiatric disorders.

PubMed

Turner, Cortney A; Eren-Koçak, Emine; Inui, Edny G; Watson, Stanley J; Akil, Huda

2016-05-01

The role of the fibroblast growth factor (FGF) system in brain-related disorders has received considerable attention in recent years. To understand the role of this system in neurological and psychiatric disorders, it is important to identify the specific members of the FGF family that are implicated, their location and the various mechanisms they can be modulated. Each disorder appears to impact specific molecular players in unique anatomical locations, and all of these could conceivably become targets for treatment. In the last several years, the issue of how to target this system directly has become an area of increasing interest. To date, the most promising therapeutics are small molecule inhibitors and antibodies that modulate FGF receptor (FGFR) function. Beyond attempting to modify the primary players affected by a given brain disorder, it may prove useful to target molecules, such as membrane-bound or extracellular proteins that interact with FGF ligands or FGFRs to modulate signaling. Published by Elsevier Ltd.

ATV Fly-Under

NASA Image and Video Library

2014-08-08

ISS040-E-089829 (8 Aug. 2014) --- The “Georges Lemaitre” Automated Transfer Vehicle (ATV-5), photographed by an Expedition 40 crew member, flies directly under the International Space Station at a distance of about 3.7 miles to test sensors and radar systems designed for future European spacecraft. After its “fly-under” of the station, the ATV will move in front of, above, and behind the outpost for the final days of its two-week rendezvous that will lead to an automated docking to the aft port of the Zvezda Service Module on Aug. 12.

ATV Fly-Under

NASA Image and Video Library

2014-08-08

ISS040-E-089793 (8 Aug. 2014) --- The “Georges Lemaitre” Automated Transfer Vehicle (ATV-5), photographed by an Expedition 40 crew member, flies directly under the International Space Station at a distance of about 3.7 miles to test sensors and radar systems designed for future European spacecraft. After its “fly-under” of the station, the ATV will move in front of, above, and behind the outpost for the final days of its two-week rendezvous that will lead to an automated docking to the aft port of the Zvezda Service Module on Aug. 12.

ATV Fly-Under

NASA Image and Video Library

2014-08-08

ISS040-E-089802 (8 Aug. 2014) --- The “Georges Lemaitre” Automated Transfer Vehicle (ATV-5), photographed by an Expedition 40 crew member, flies directly under the International Space Station at a distance of about 3.7 miles to test sensors and radar systems designed for future European spacecraft. After its “fly-under” of the station, the ATV will move in front of, above, and behind the outpost for the final days of its two-week rendezvous that will lead to an automated docking to the aft port of the Zvezda Service Module on Aug. 12.

ATV Fly-Under

NASA Image and Video Library

2014-08-08

ISS040-E-089782 (8 Aug. 2014) --- The “Georges Lemaitre” Automated Transfer Vehicle (ATV-5), photographed by an Expedition 40 crew member, flies directly under the International Space Station at a distance of about 3.7 miles to test sensors and radar systems designed for future European spacecraft. After its “fly-under” of the station, the ATV will move in front of, above, and behind the outpost for the final days of its two-week rendezvous that will lead to an automated docking to the aft port of the Zvezda Service Module on Aug. 12.

ATV Fly-Under

NASA Image and Video Library

2014-08-08

ISS040-E-089830 (8 Aug. 2014) --- The “Georges Lemaitre” Automated Transfer Vehicle (ATV-5), photographed by an Expedition 40 crew member, flies directly under the International Space Station at a distance of about 3.7 miles to test sensors and radar systems designed for future European spacecraft. After its “fly-under” of the station, the ATV will move in front of, above, and behind the outpost for the final days of its two-week rendezvous that will lead to an automated docking to the aft port of the Zvezda Service Module on Aug. 12.

ATV Fly-Under

NASA Image and Video Library

2014-08-08

ISS040-E-089820 (8 Aug. 2014) --- The “Georges Lemaitre” Automated Transfer Vehicle (ATV-5), photographed by an Expedition 40 crew member, flies directly under the International Space Station at a distance of about 3.7 miles to test sensors and radar systems designed for future European spacecraft. After its “fly-under” of the station, the ATV will move in front of, above, and behind the outpost for the final days of its two-week rendezvous that will lead to an automated docking to the aft port of the Zvezda Service Module on Aug. 12.

ATV Fly-Under

NASA Image and Video Library

2014-08-08

ISS040-E-089798 (8 Aug. 2014) --- The “Georges Lemaitre” Automated Transfer Vehicle (ATV-5), photographed by an Expedition 40 crew member, flies directly under the International Space Station at a distance of about 3.7 miles to test sensors and radar systems designed for future European spacecraft. After its “fly-under” of the station, the ATV will move in front of, above, and behind the outpost for the final days of its two-week rendezvous that will lead to an automated docking to the aft port of the Zvezda Service Module on Aug. 12.

Currie and Krikalev pull launch restraint bolts in the FGB/Zarya module

NASA Image and Video Library

2013-11-19

STS088-359-037 (4-15 Dec. 1998) --- Astronaut Nancy J. Currie and cosmonaut Sergei K. Krikalev, both mission specialists, use rechargeable power tools to manipulate nuts and bolts on the Russian-built Zarya module. Astronaut Robert D. Cabana, mission commander, translates along the rail network in the background. The six STS-88 crew members had earlier entered the module through the U.S.-built Unity connecting module. Rails, straps and tools indicate the crewmembers had been working awhile when this photo was taken. Krikalev, representing the Russian Space Agency (RSA), has been assigned as a member of the three-man initial International Space Station (ISS) crew.

Capacity building from the inside out: development and evaluation of a CITI ethics certification training module for American Indian and Alaska Native community researchers.

PubMed

Pearson, Cynthia R; Parker, Myra; Fisher, Celia B; Moreno, Claudia

2014-02-01

Current human subject research training modules fail to capture ethically relevant cultural aspects of research involving American Indian and Alaska Native (AI/AN) community members. Applying a Community Engaged Research (CEnR) approach, we adapted the Collaborative IRB Training Initiative training module "assessing risk and benefits." In a two-arm randomized controlled trial, followed by debriefing interviews, we evaluated module acceptability and understandability (test scores) among 40 reservation-based community members. Participants who took the adapted module, compared to those who took the standard module, reported higher scores on relevance of the material overall satisfaction, module quiz scores, and a trend toward higher self-efficacy. Implications of the efficacy of this approach for enhancing ethics training and community participation in research within AI/AN and other cultural populations within and outside the United States are discussed.

Interdisciplinary Integration of the CVS Module and Its Effect on Faculty and Student Satisfaction as Well as Student Performance

PubMed Central

2012-01-01

Background Beyond the adoption of the principles of horizontal and vertical integration, significant planning and implementation of curriculum reform is needed. This study aimed to assess the effect of the interdisciplinary integrated Cardiovascular System (CVS) module on both student satisfaction and performance and comparing them to those of the temporally coordinated CVS module that was implemented in the previous year at the faculty of Medicine of the King Abdulaziz University, Saudi Arabia. Methods This interventional study used mixed method research design to assess student and faculty satisfaction with the level of integration within the CVS module. A team from the medical education department was assembled in 2010/2011 to design a plan to improve the CVS module integration level. After delivering the developed module, both student and faculty satisfaction as well as students performance were assessed and compared to those of the previous year to provide an idea about module effectiveness. Results Many challenges faced the medical education team during design and implementation of the developed CVS module e.g. resistance of faculty members to change, increasing the percentage of students directed learning hours from the total contact hour allotted to the module and shifting to integrated item writing in students assessment, spite of that the module achieved a significant increase in both teaching faculty and student satisfaction as well as in the module scores. Conclusion The fully integrated CVS has yielded encouraging results that individual teachers or other medical schools who attempt to reformulate their curriculum may find valuable. PMID:22747781

Multiple melanocortin receptors are expressed in bone cells

NASA Technical Reports Server (NTRS)

Zhong, Qing; Sridhar, Supriya; Ruan, Ling; Ding, Ke-Hong; Xie, Ding; Insogna, Karl; Kang, Baolin; Xu, Jianrui; Bollag, Roni J.; Isales, Carlos M.

2005-01-01

Melanocortin receptors belong to the seven transmembrane domain, G-protein coupled family of receptors. There are five members of this receptor family labeled MC1R-MC5R. These receptors are activated by fragments derived from a larger molecule, proopiomelanocortin (POMC) and include ACTH, alpha beta and gamma-MSH and beta-endorphin. Because of in vitro and in vivo data suggesting direct effects of these POMC molecules on bone and bone turnover, we examined bone and bone derived cells for the presence of the various members of the melanocortin receptor family. We report that the five known melanocortin receptors are expressed to varying degrees in osteoblast-like and osteoclastic cells. POMC fragments increased proliferation and expression of a variety of genes in osteoblastic cells. Furthermore, POMC mRNA was detected in osteoclastic cells. These data demonstrate that POMC-derived peptide hormones acting through high affinity melanocortin receptors have specific effects on bone cells. Thus, in addition to the indirect effects of POMC-derived hormones on bone turnover through their modulation of steroid hormone secretion, POMC fragments may have direct and specific effects on bone cell subpopulations.

International Space Station (ISS)

NASA Image and Video Library

2001-01-01

This is the STS-102 mission crew insignia. The central image on the crew patch depicts the International Space Station (ISS) in the build configuration that it had at the time of the arrival and docking of Discovery during the STS-102 mission, the first crew exchange flight to the Space Station. The station is shown along the direction of the flight as was seen by the shuttle crew during their final approach and docking, the so-called V-bar approach. The names of the shuttle crew members are depicted in gold around the top of the patch, and surnames of the Expedition crew members being exchanged are shown in the lower barner. The three ribbons swirling up to and around the station signify the rotation of these ISS crew members. The number 2 is for the Expedition 2 crew who flew up to the station, and the number 1 is for the Expedition 1 crew who then returned down to Earth. In conjunction with the face of the Lab module of the Station, these Expedition numbers create the shuttle mission number 102. Shown mated below the ISS is the Italian-built Multipurpose Logistics Module, Leonardo, that flew for the first time on this flight. The flags of the countries that were the major contributors to this effort, the United States, Russia, and Italy are also shown in the lower part of the patch. The build-sequence number of this flight in the overall station assembly sequence, 5A.1, is captured by the constellations in the background.

Librarian-Faculty Collaboration on a Library Research Assignment and Module for College Experience Classes

ERIC Educational Resources Information Center

Keyes, Anne; Barbier, Pat

2013-01-01

A librarian and faculty member collaborated on creating a library research module for students in the faculty member's college success classes to help them learn the fundamentals of information literacy. Using the assignment "My Ideal Job," the students met four or more times with the librarian in a computer classroom to learn how to do…

miR-223/Hsp70/JNK/JUN/miR-223 feedback loop modulates the chemoresistance of osteosarcoma to cisplatin.

PubMed

Tang, Qi; Yuan, Qi; Li, Hui; Wang, Wanchun; Xie, Guangrong; Zhu, Kewei; Li, Ding

2018-03-11

Osteosarcoma (OS) is a primary bone malignancy with a five-year survival rate of 60%; the chemoresistance of OS still remains a huge challenge. Heat shock protein 70 (Hsp70), a member of HSP family, is overexpressed in OS cell lines and involved in the resistance of OS cell lines. In addition, miRNAs have been involved in the carcinogenesis and chemoresistance of OS; of them, miR-223 has been reported to be underexpressed and serve as a tumor suppressor in OS through targeting Hsp90B1, also a member of HSP family. Herein, online tools predicted that Hsp70 might be a direct target of miR-223. In the present study, miR-223 expression was down-regulated in OS tissues and cell lines; miR-223 overexpression enhanced the cellular effects of cisplatin (CDDP) on OS cell lines. Through binding to the HSPA1A 3'UTR, miR-223 could regulate Hsp70 protein levels and downstream JNK/JUN signaling pathway, thus modulating OS cell apoptosis through Hsp70 under CDDP stress. Finally, JUN, a downstream transcription factor of JNK signaling, could bind to the promoter region of miR-223 to promote its transcription. In summary, miR-223, Hsp70 and downstream JNK/JUN formed a feedback loop to modulate the chemoresistance of OS to CDDP. Copyright © 2018 Elsevier Inc. All rights reserved.

Thermoelectric generator with hinged assembly for fins

DOEpatents

Purdy, David L.; Shapiro, Zalman M.; Hursen, Thomas F.; Maurer, Gerould W.

1976-11-02

A cylindrical casing has a central shielded capsule of radioisotope fuel. A plurality of thermonuclear modules are axially arranged with their hot junctions resiliently pressed toward the shield and with their cold junctions adjacent a transition member having fins radiating heat to the environment. For each module, the assembly of transition member and fins is hinged to the casing for swinging to permit access to and removal of such module. A ceramic plate having gold layers on opposite faces prevents diffusion bonding of the hot junction to the shield.

Thermoelectric generator having a resiliently mounted removable thermoelectric module

DOEpatents

Purdy, David L.; Shapiro, Zalman M.; Hursen, Thomas F.; Maurer, Gerould W.

1976-11-02

An electrical generator having an Isotopic Heat Capsule including radioactive fuel rod 21 as a primary heat source and Thermoelectric Modules 41 and 43 as converters. The Biological Shield for the Capsule is suspended from Spiders at each end each consisting of pretensioned rods 237 and 239 defining planes at right angles to each other. The Modules are mounted in cups 171 of transition members 173 of a heat rejection Fin Assembly whose fins 195 and 197 extend from both sides of the transition member 173 for effective cooling.

Concentrating photovoltaic solar panel

DOEpatents

Cashion, Steven A; Bowser, Michael R; Farrelly, Mark B; Hines, Braden E; Holmes, Howard C; Johnson, Jr., Richard L; Russell, Richard J; Turk, Michael F

2014-04-15

The present invention relates to photovoltaic power systems, photovoltaic concentrator modules, and related methods. In particular, the present invention features concentrator modules having interior points of attachment for an articulating mechanism and/or an articulating mechanism that has a unique arrangement of chassis members so as to isolate bending, etc. from being transferred among the chassis members. The present invention also features adjustable solar panel mounting features and/or mounting features with two or more degrees of freedom. The present invention also features a mechanical fastener for secondary optics in a concentrator module.

Fisheye view of Atlantis from Mir space station

NASA Image and Video Library

1995-06-29

STS071-741-004 (27 June-7 July 1995) --- Docked already with Russia's Mir Space Station and backdropped against a half globe of Earth featuring the Crimean Peninsula, the space shuttle Atlantis is partially visible through a window on the Kvant 2 Module. A 70mm camera, carried into space by the STS-71 crew aboard the space shuttle Atlantis, was used to expose the image. The crew cabin and forward cargo bay of Atlantis are most prominent. Below center can be seen the Androgynous Peripheral Docking System (APDS) and the Kristall Module on Mir. The APDS is connected to a port in a tunnel leading to the Spacelab Science Module in Atlantis' cargo bay. The linkup enabled the seven STS-71 crew members to visit Mir and it allowed the three Mir-18 crew members, in space since March of this year, access to Spacelab. That module was quite busy with tests and data collection involving the three, Mir-18 crew, until Atlantis brought them home on July 7, 1995. The Black Sea lies directly beneath Atlantis, with Ukraine's diamond-shaped Crimean Peninsula immediately to the right of the cockpit. The wide lower course of the Dnepr River can be seen entering the Black Sea at far right. The coast of Romania and Bulgaria lies at a point where the cloud begins at top right. The peninsula of Asia Minor lies across the left of the view, mostly under cloud cover. The Mediterranean Sea is the cloud-free, blue mass beyond. Still further, at about 1,300 miles distance, the north edge of Africa is stretched out as a line across the horizon with its characteristic sandy color. The nose of Atlantis points southwest toward the only outlet of the Black Sea known as the Bosporus.

The alpha subunit of Go interacts with promyelocytic leukemia zinc finger protein and modulates its functions.

PubMed

Won, Jung Hee; Park, Jung Sik; Ju, Hyun Hee; Kim, Soyeon; Suh-Kim, Haeyoung; Ghil, Sung Ho

2008-05-01

Heterotrimeric GTP-binding proteins (G proteins) mediate signal transduction generated by neurotransmitters and hormones. Go, a member of the Go/Gi family, is the most abundant heterotrimeric G protein in the brain. Most mechanistic analyses on Go activation demonstrate that its action is mediated by the Gbetagamma dimer; downstream effectors for its alpha subunit (Goalpha) have not been clearly defined. Here, we employ the yeast two-hybrid system to screen for Goalpha-interacting partners in a cDNA library from human fetal brain. The transcription factor promyelocytic leukemia zinc finger protein (PLZF) specifically bound to Goalpha. Interactions between PLZF and Goalpha were confirmed using in vitro and in vivo affinity binding assays. Activated Goalpha interacted directly with PLZF, and enhanced its function as a transcriptional and cell growth suppressor. Notably, PLZF activity was additionally promoted by the Go/ialpha-coupled cannabinoid receptor (CB) in HL60 cells endogenously expressing CB and PLZF. These results collectively suggest that Goalpha modulates the function of PLZF via direct interactions. Our novel findings provide insights into the diverse cellular roles of Goalpha and its coupled receptor.

Traumatic Brain Injury: A Guide for Caregivers of Service Members and Veterans

DTIC Science & Technology

2010-01-01

Fracture - Module 1, pages 2, 15, 22, 26...pages 6, 9, 13-16, 20, 22, 25, 27 Skull Fracture - Companion, page 9; Module 1, pages 14-15, 22, 26 Simple Fracture - Module 1, page 15 Compound... Fracture - Module 1, page 15 Depressed Skull Fracture - Module 1, page 15 Basal Skull Fracture - Module 1, page 15 Sleep Changes - Module 2, pages

The DAN family: modulators of TGF-β signaling and beyond.

PubMed

Nolan, Kristof; Thompson, Thomas B

2014-08-01

Extracellular binding proteins or antagonists are important factors that modulate ligands in the transforming growth factor (TGF-β) family. While the interplay between antagonists and ligands are essential for developmental and normal cellular processes, their imbalance can lead to the pathology of several disease states. In particular, recent studies have implicated members of the differential screening-selected gene in neuroblastoma (DAN) family in disease such as renal fibrosis, pulmonary arterial hypertension, and reactivation of metastatic cancer stem cells. DAN family members are known to inhibit the bone morphogenetic proteins (BMP) of the TGF-β family. However, unlike other TGF-β antagonist families, DAN family members have roles beyond ligand inhibition and can modulate Wnt and vascular endothelial growth factor (VEGF) signaling pathways. This review describes recent structural and functional advances that have expanded our understanding of DAN family proteins with regards to BMP inhibition and also highlights their emerging roles in the modulation of Wnt and VEGF signaling pathways. © 2014 The Protein Society.

Future Directions for The Math You Need, When You Need It: Adaptation and Implementation of Online Student-Centered Tutorials that Remediate Introductory Geoscience-Related Mathematical Skills

NASA Astrophysics Data System (ADS)

Wenner, J. M.; Burn, H.; Baer, E. M.

2009-12-01

Requiring introductory geoscience students to apply mathematical concepts and solve quantitative problems can be an arduous task because these courses tend to attract students with diverse levels of mathematical preparedness. Perhaps more significantly, geoscience instructors grapple with quantitative content because of the difficulties students have transferring their prior mathematical learning to common geological problems. As a result, instructors can choose to eliminate the mathematics, spend valuable class time teaching basic mathematical skills or let students flounder in the hope that they will learn on their own. None of these choices are ideal. Instead, research suggests that introductory geoscience courses are opportune places to increase students’ quantitative abilities but that students need effective support at their own skill level. To provide such support, we developed The Math You Need, When You Need It (TMYN): a set of online geoscience context-rich tutorials that students complete just before they encounter a mathematical or numerical skill in their introductory course. The tutorials are modular; each mathematical topic has a set of pages that students work through toward a final assessment. The 11 modules currently available, including unit conversions, graphing, calculating density, and rearranging equations, touch on quantitative topics that cross a number of geologic contexts. TMYN modules are designed to be stand-alone and flexible - faculty members can choose modules appropriate for their courses and implement them at any time throughout the term. The flexible and adaptable nature of TMYN enables faculty to provide a supportive learning environment that remediates math for those who need it without taking significant classroom time. Since spring 2008, seven instructors at Highline Community College and University of Wisconsin Oshkosh successfully implemented TMYN in six geoscience courses with diverse student audiences. Evaluation of pilot implementations suggests that the flexibility of TYMN is one of its strengths. Specifically, faculty members responded positively to the ability to choose relevant topics and provide students with competence in pertinent mathematical concepts; students liked the supportive, contextual environment and the ability to work at their own pace. And, despite the fact that each implementation varied in the number and type of modules used, the timing of module use, grading stakes, and course size, pre/post test results consistently showed improvement in student skills associated with a given module, suggesting that all implementations were successful. Post-module surveys likewise revealed that both instructors and students found the experience valuable. We present the wide variety of successful implementations with an eye toward exploring future directions for the project, including soliciting new and diverse ways in which other institutions and instructors might adapt and apply TMYN to their own courses.

Development of shashlik electromagnetic calorimeter prototype for SoLID

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shen, C.; Wang, Y.; Xiao, D.

A shashlik electromagnetic calorimeter will be produced in Hall A of Jefferson Laboratory for Solenoidal large Intensity Device (SoLID) to measure the energy deposition of electrons and hadrons, and to provide particle identification after the energy of the accelerator was upgraded to 12 GeV. Tsinghua University is the member of Hall A collaboration in charge of development and production of the large shashlik electromagnetic calorimeter of SoLID. One module of that calorimeter is composed by 194 layers. Each layer consists of a 1.5 mm thick plastic scintillator put on top of a 0.5 mm thick lead plate. Scintillation light ismore » read out by wave-length shifter fibers penetrating through the calorimeter modules longitudinally along the direction of flight of the impact particle. This paper describes the design and construction of that module, as well as a few optimization studies meant to improve its performance. A detailed Geant4 simulation also shows that an energy resolution of 5%/√ E (GeV) and a good containment for electromagnetic showers can be achieved, as well as some basic electron identification. In conclusion, a prototype of that module will be tested soon with an electron beam at JLab.« less

Development of shashlik electromagnetic calorimeter prototype for SoLID

DOE PAGES

Shen, C.; Wang, Y.; Xiao, D.; ...

2017-03-07

A shashlik electromagnetic calorimeter will be produced in Hall A of Jefferson Laboratory for Solenoidal large Intensity Device (SoLID) to measure the energy deposition of electrons and hadrons, and to provide particle identification after the energy of the accelerator was upgraded to 12 GeV. Tsinghua University is the member of Hall A collaboration in charge of development and production of the large shashlik electromagnetic calorimeter of SoLID. One module of that calorimeter is composed by 194 layers. Each layer consists of a 1.5 mm thick plastic scintillator put on top of a 0.5 mm thick lead plate. Scintillation light ismore » read out by wave-length shifter fibers penetrating through the calorimeter modules longitudinally along the direction of flight of the impact particle. This paper describes the design and construction of that module, as well as a few optimization studies meant to improve its performance. A detailed Geant4 simulation also shows that an energy resolution of 5%/√ E (GeV) and a good containment for electromagnetic showers can be achieved, as well as some basic electron identification. In conclusion, a prototype of that module will be tested soon with an electron beam at JLab.« less

Apparatus for Generating Thrust Using a Two Dimensional, Asymmetrical Capacitor Module

NASA Technical Reports Server (NTRS)

Campbell, Jonathan W. (Inventor)

2002-01-01

An asymmetrical capacitor module for generating thrust includes two conductive elements of similar but different geometries separated by a dielectric member. Improved embodiments provided in the construction of conductive elements of smaller axial extent include those where the element is formed by an annular wire or a dielectric supported ring. Other embodiments concern the dielectric member and involve changes in the extent and shape thereof.

The oncogenic tyrosine kinase Lyn impairs the pro-apoptotic function of Bim.

PubMed

Aira, Lazaro E; Villa, Elodie; Colosetti, Pascal; Gamas, Parvati; Signetti, Laurie; Obba, Sandrine; Proics, Emma; Gautier, Fabien; Bailly-Maitre, Béatrice; Jacquel, Arnaud; Robert, Guillaume; Luciano, Frédéric; Juin, Philippe P; Ricci, Jean-Ehrland; Auberger, Patrick; Marchetti, Sandrine

2018-04-01

Phosphorylation of Ser/Thr residues is a well-established modulating mechanism of the pro-apoptotic function of the BH3-only protein Bim. However, nothing is known about the putative tyrosine phosphorylation of this Bcl-2 family member and its potential impact on Bim function and subsequent Bax/Bak-mediated cytochrome c release and apoptosis. As we have previously shown that the tyrosine kinase Lyn could behave as an anti-apoptotic molecule, we investigated whether this Src family member could directly regulate the pro-apoptotic function of Bim. In the present study, we show that Bim is phosphorylated onto tyrosine residues 92 and 161 by Lyn, which results in an inhibition of its pro-apoptotic function. Mechanistically, we show that Lyn-dependent tyrosine phosphorylation of Bim increases its interaction with anti-apoptotic members such as Bcl-xL, therefore limiting mitochondrial outer membrane permeabilization and subsequent apoptosis. Collectively, our data uncover one molecular mechanism through which the oncogenic tyrosine kinase Lyn negatively regulates the mitochondrial apoptotic pathway, which may contribute to the transformation and/or the chemotherapeutic resistance of cancer cells.

Interprofessional education in a primary care teaching clinic: findings from a study involving pharmacy and medical students.

PubMed

Sicat, Brigitte Luong; Huynh, Christine; Willett, Rita; Polich, Susan; Mayer, Sallie

2014-01-01

Interprofessional education (IPE) can be hindered by the lack of infrastructure required to support it. We developed a clinical IPE experience for medical and pharmacy students built upon an existing infrastructure. We created tools to orient students to IPE and had students participate in pharmacist-led and physician-led IPE clinics. Results from the surveys indicated that after participating in the IPE experience, there were no significant changes in attitudes toward interprofessional teamwork or attitudes toward different members of the healthcare team. Students found less value in tools outlining roles and responsibilities of team members, on-line modules about the other profession, and IPE group discussion. They placed more value on the actual clinical experience. Themes derived from analysis of open-ended survey questions reflected the value that students placed on interprofessional interaction in the setting of direct patient care.

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

PubMed Central

Gielen, Fabrice; Hours, Raphaelle; Emond, Stephane; Fischlechner, Martin; Schell, Ursula

2016-01-01

Ultrahigh-throughput screening, in which members of enzyme libraries compartmentalized in water-in-oil emulsion droplets are assayed, has emerged as a powerful format for directed evolution and functional metagenomics but is currently limited to fluorescence readouts. Here we describe a highly efficient microfluidic absorbance-activated droplet sorter (AADS) that extends the range of assays amenable to this approach. Using this module, microdroplets can be sorted based on absorbance readout at rates of up to 300 droplets per second (i.e., >1 million droplets per hour). To validate this device, we implemented a miniaturized coupled assay for NAD+-dependent amino acid dehydrogenases. The detection limit (10 μM in a coupled assay producing a formazan dye) enables accurate kinetic readouts sensitive enough to detect a minimum of 1,300 turnovers per enzyme molecule, expressed in a single cell, and released by lysis within a droplet. Sorting experiments showed that the AADS successfully enriched active variants up to 2,800-fold from an overwhelming majority of inactive ones at ∼100 Hz. To demonstrate the utility of this module for protein engineering, two rounds of directed evolution were performed to improve the activity of phenylalanine dehydrogenase toward its native substrate. Fourteen hits showed increased activity (improved >4.5-fold in lysate; kcat increased >2.7-fold), soluble protein expression levels (up 60%), and thermostability (Tm, 12 °C higher). The AADS module makes the most widely used optical detection format amenable to screens of unprecedented size, paving the way for the implementation of chromogenic assays in droplet microfluidics workflows. PMID:27821774

Long-range sclerotome induction by sonic hedgehog: direct role of the amino-terminal cleavage product and modulation by the cyclic AMP signaling pathway.

PubMed

Fan, C M; Porter, J A; Chiang, C; Chang, D T; Beachy, P A; Tessier-Lavigne, M

1995-05-05

A long-range signal encoded by the Sonic hedgehog (Shh) gene has been implicated as the ventral patterning influence from the notochord that induces sclerotome and represses dermomyotome in somite differentiation. Long-range effects of hedgehog (hh) signaling have been suggested to result either from local induction of a secondary diffusible signal or from the direct action of the highly diffusible carboxy-terminal product of HH autoproteolytic cleavage. Here we provide evidence that the long-range somite patterning effects of SHH are instead mediated by a direct action of the amino-terminal cleavage product. We also show that pharmacological manipulations to increase the activity of cyclic AMP-dependent protein kinase A can selectively antagonize the effects of the amino-terminal cleavage product. Our results support the operation of a single evolutionarily conserved signaling pathway for both local and direct long-range inductive actions of HH family members.

miR-30 Family Members Negatively Regulate Osteoblast Differentiation*

PubMed Central

Wu, Tingting; Zhou, Haibo; Hong, Yongfeng; Li, Jing; Jiang, Xinquan; Huang, Hui

2012-01-01

miRNAs are endogenously expressed 18- to 25-nucleotide RNAs that regulate gene expression through translational repression by binding to a target mRNA. Recently, it has been indicated that miRNAs are closely related to osteogenesis. Our previous data suggested that miR-30 family members might be important regulators during the biomineralization process. However, whether and how they modulate osteogenic differentiation have not been explored. In this study, we demonstrated that miR-30 family members negatively regulate BMP-2-induced osteoblast differentiation by targeting Smad1 and Runx2. Evidentially, overexpression of miR-30 family members led to a decrease of alkaline phosphatase activity, whereas knockdown of them increased the activity. Then bioinformatic analysis identified potential target sites of the miR-30 family located in the 3′ untranslated regions of Smad1 and Runx2. Western blot analysis and quantitative RT-PCR assays demonstrated that miR-30 family members inhibit Smad1 gene expression on the basis of repressing its translation. Furthermore, dual-luciferase reporter assays confirmed that Smad1 is a direct target of miR-30 family members. Rescue experiments that overexpress Smad1 and Runx2 significantly eliminated the inhibitory effect of miR-30 on osteogenic differentiation and provided strong evidence that miR-30 mediates the inhibition of osteogenesis by targeting Smad1 and Runx2. Also, the inhibitory effects of the miR-30 family were validated in mouse bone marrow mesenchymal stem cells. Therefore, our study uncovered that miR-30 family members are key negative regulators of BMP-2-mediated osteogenic differentiation. PMID:22253433

View of Apollo 14 crewmen in Command Module simulation training

NASA Image and Video Library

1970-07-15

S70-45580 (July 1970) --- The members of the prime crew of the Apollo 14 lunar landing mission participate in Command Module (CM) simulation training at the Kennedy Space Center (KSC). Left to right are astronauts Edgar D. Mitchell, lunar module pilot; Stuart A. Roosa, command module pilot; and Alan B. Shepard Jr., commander.

TEM study of the (SbS){sub 1+δ}(NbS{sub 2}){sub n}, (n=1, 2, 3; δ~1.14, 1.20) misfit layer phases

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gómez-Herrero, A., E-mail: adriangh@pdi.ucm.es; Landa-Cánovas, A.R.; Otero-Díaz, L.C.

In the Sb–Nb–S system four new misfit layer phases have been found and carefully investigated via Transmission Electron Microscopy (TEM). Their structures are of composite modulated structure type with stoichiometries that can be formulated as (SbS){sub 1+δ}(NbS{sub 2}){sub n}; for n=1, δ~1.14 and 1.19; for n=2, δ~1.18 and for n=3, δ~1.19. Selected Area Electron Diffraction (SAED) patterns show an almost commensurate fit between the pseudo-tetragonal (SbS) and the pseudo-orthohexagonal (NbS{sub 2}){sub n} subcells along the misfit direction a, with 3(SbS)≈5(NbS{sub 2}), being b the same for both sub-lattices and c the stacking direction. For n=1, a commensurate phase with 4a{submore » SbS}=7a{sub NbS2} has also been observed. In addition to the characteristic misfit and associated modulation of the two sub-structures, a second modulation is also present which appears to be primarily associated with the (SbS) sub-structure of both the n=1 and n=2 phases. High Resolution Transmission Electron Microscopy (HRTEM) images show ordered stacking sequences between the (SbS) and (NbS{sub 2}){sub n} lamellae for each of the four phases, however, disordered intergrowths were also occasionally found. Most of the crystals showed different kinds of twinning defects on quite a fine scale. Many crystals showed curled up edges. In some cases the lamellar crystals were entirely folded giving rise to similar diffraction patterns as found for cylindrical crystals. - Graphical abstract: Idealized structure models of the first three members of the homologous series (SbS){sub 1+δ}(NbS{sub 2}){sub n}. - Highlights: • Transmission Electron Microscopy study of misfit layer sulfides (SbS){sub 1+δ}(NbS{sub 2}){sub n}. • The structures consist of a (SbS) layer interleaved between n (NbS{sub 2}) layers. • Two different members n=1, one n=2 and one n=3 have been studied. • Twinning, intergrowths and different modulations in the (SbS) substructure.« less

A multitasking Argonaute: exploring the many facets of C. elegans CSR-1.

PubMed

Wedeles, Christopher J; Wu, Monica Z; Claycomb, Julie M

2013-12-01

While initial studies of small RNA-mediated gene regulatory pathways focused on the cytoplasmic functions of such pathways, identifying roles for Argonaute/small RNA pathways in modulating chromatin and organizing the genome has become a topic of intense research in recent years. Nuclear regulatory mechanisms for Argonaute/small RNA pathways appear to be widespread, in organisms ranging from plants to fission yeast, Caenorhabditis elegans to humans. As the effectors of small RNA-mediated gene regulatory pathways, Argonaute proteins guide the chromatin-directed activities of these pathways. Of particular interest is the C. elegans Argonaute, chromosome segregation and RNAi deficient (CSR-1), which has been implicated in such diverse functions as organizing the holocentromeres of worm chromosomes, modulating germline chromatin, protecting the genome from foreign nucleic acid, regulating histone levels, executing RNAi, and inhibiting translation in conjunction with Pumilio proteins. CSR-1 interacts with small RNAs known as 22G-RNAs, which have complementarity to 25 % of the protein coding genes. This peculiar Argonaute is the only essential C. elegans Argonaute out of 24 family members in total. Here, we summarize the current understanding of CSR-1 functions in the worm, with emphasis on the chromatin-directed activities of this ever-intriguing Argonaute.

Astronaut Fred Haise - Suiting Room - Prelaunch - KSC

NASA Image and Video Library

1970-04-11

S70-34851 (11 April 1970) --- A space suit technician talks with astronaut Fred W. Haise Jr., lunar module pilot for NASA's Apollo 13 mission, during suiting up procedures at Kennedy Space Center (KSC). Other members of the crew are astronauts James A. Lovell Jr., commander, and John L. Swigert Jr., command module pilot. Swigert replaced astronaut Thomas K. Mattingly II as a member of the crew when it was learned he had been exposed to measles.

A Standardized Certification Program for Case Managers Serving Frail Elderly Texans. Module II: Assessment and Care Plan Development.

ERIC Educational Resources Information Center

Lusky, Richard A.; And Others

This learning module is one of three training modules that were developed for members of the Texas Gerontological Consortium for Continuing Education to use in preparing case managers working in human service professions coordinating community-based programs for frail elderly Texans. Module II deals with the following topics: assessment (role of…

HYPE: a WFD tool for the identification of significant and sustained upward trends in groundwater time series

NASA Astrophysics Data System (ADS)

Lopez, Benjamin; Croiset, Nolwenn; Laurence, Gourcy

2014-05-01

The Water Framework Directive 2006/11/CE (WFD) on the protection of groundwater against pollution and deterioration asks Member States to identify significant and sustained upward trends in all bodies or groups of bodies of groundwater that are characterised as being at risk in accordance with Annex II to Directive 2000/60/EC. The Directive indicates that the procedure for the identification of significant and sustained upward trends must be based on a statistical method. Moreover, for significant increases of concentrations of pollutants, trend reversals are identified as being necessary. This means to be able to identify significant trend reversals. A specific tool, named HYPE, has been developed in order to help stakeholders working on groundwater trend assessment. The R encoded tool HYPE provides statistical analysis of groundwater time series. It follows several studies on the relevancy of the use of statistical tests on groundwater data series (Lopez et al., 2011) and other case studies on the thematic (Bourgine et al., 2012). It integrates the most powerful and robust statistical tests for hydrogeological applications. HYPE is linked to the French national database on groundwater data (ADES). So monitoring data gathered by the Water Agencies can be directly processed. HYPE has two main modules: - a characterisation module, which allows to visualize time series. HYPE calculates the main statistical characteristics and provides graphical representations; - a trend module, which identifies significant breaks, trends and trend reversals in time series, providing result table and graphical representation (cf figure). Additional modules are also implemented to identify regional and seasonal trends and to sample time series in a relevant way. HYPE has been used successfully in 2012 by the French Water Agencies to satisfy requirements of the WFD, concerning characterization of groundwater bodies' qualitative status and evaluation of the risk of non-achievement of good status. Bourgine B. et al. 2012, Ninth International Geostatistics Congress, Oslo, Norway June 11 - 15. Lopez B. et al. 2011, Final Report BRGM/RP-59515-FR. 166p.

Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae

PubMed Central

Hierlmeier, Thomas; Merl, Juliane; Sauert, Martina; Perez-Fernandez, Jorge; Schultz, Patrick; Bruckmann, Astrid; Hamperl, Stephan; Ohmayer, Uli; Rachel, Reinhard; Jacob, Anja; Hergert, Kristin; Deutzmann, Rainer; Griesenbeck, Joachim; Hurt, Ed; Milkereit, Philipp; Baßler, Jochen; Tschochner, Herbert

2013-01-01

Eukaryotic ribosome biogenesis requires more than 150 auxiliary proteins, which transiently interact with pre-ribosomal particles. Previous studies suggest that several of these biogenesis factors function together as modules. Using a heterologous expression system, we show that the large ribosomal subunit (LSU) biogenesis factor Noc1p of Saccharomyces cerevisiae can simultaneously interact with the LSU biogenesis factor Noc2p and Rrp5p, a factor required for biogenesis of the large and the small ribosomal subunit. Proteome analysis of RNA polymerase-I-associated chromatin and chromatin immunopurification experiments indicated that all members of this protein module and a specific set of LSU biogenesis factors are co-transcriptionally recruited to nascent ribosomal RNA (rRNA) precursors in yeast cells. Further ex vivo analyses showed that all module members predominantly interact with early pre-LSU particles after the initial pre-rRNA processing events have occurred. In yeast strains depleted of Noc1p, Noc2p or Rrp5p, levels of the major LSU pre-rRNAs decreased and the respective other module members were associated with accumulating aberrant rRNA fragments. Therefore, we conclude that the module exhibits several binding interfaces with pre-ribosomes. Taken together, our results suggest a co- and post-transcriptional role of the yeast Rrp5p–Noc1p–Noc2p module in the structural organization of early LSU precursors protecting them from non-productive RNase activity. PMID:23209026

KSC-98pc1799

NASA Image and Video Library

1998-12-04

STS-88 Mission Specialist Jerry L. Ross is assisted with his ascent and re-entry flight suit in the white room at Launch Pad 39A before entering Space Shuttle Endeavour for launch. During the nearly 12-day mission, the six-member crew will mate the first two elements of the International Space Station the already-orbiting Zarya control module with the Unity connecting module carried by Endeavour. He is making his sixth spaceflight and is one of two extravehicular activity crew members on this mission

Regulation of Bacteria-Induced Intercellular Adhesion Molecule-1 by CCAAT/Enhancer Binding Proteins

PubMed Central

Manzel, Lori J.; Chin, Cecilia L.; Behlke, Mark A.; Look, Dwight C.

2009-01-01

Direct interaction between bacteria and epithelial cells may initiate or amplify the airway response through induction of epithelial defense gene expression by nuclear factor-κB (NF-κB). However, multiple signaling pathways modify NF-κB effects to modulate gene expression. In this study, the effects of CCAAT/enhancer binding protein (C/EBP) family members on induction of the leukocyte adhesion glycoprotein intercellular adhesion molecule-1 (ICAM-1) was examined in primary cultures of human tracheobronchial epithelial cells incubated with nontypeable Haemophilus influenzae. Increased ICAM-1 gene transcription in response to H. influenzae required gene sequences located at −200 to −135 in the 5′-flanking region that contain a C/EBP-binding sequence immediately upstream of the NF-κB enhancer site. Constitutive C/EBPβ was found to have an important role in epithelial cell ICAM-1 regulation, while the adjacent NF-κB sequence binds the RelA/p65 and NF-κB1/p50 members of the NF-κB family to induce ICAM-1 expression in response to H. influenzae. The expression of C/EBP proteins is not regulated by p38 mitogen-activated protein kinase activation, but p38 affects gene transcription by increasing the binding of TATA-binding protein to TATA-box–containing gene sequences. Epithelial cell ICAM-1 expression in response to H. influenzae was decreased by expressing dominant-negative protein or RNA interference against C/EBPβ, confirming its role in ICAM-1 regulation. Although airway epithelial cells express multiple constitutive and inducible C/EBP family members that bind C/EBP sequences, the results indicate that C/EBPβ plays a central role in modulation of NF-κB–dependent defense gene expression in human airway epithelial cells after exposure to H. influenzae. PMID:18703796

A Database Training Module for Nassau Community College Staff and Faculty.

ERIC Educational Resources Information Center

Goodman, Harriett Ziskin

A training module developed following the Instructional System Design model was implemented at Nassau Community College (NCC) to teach its administration, faculty, and staff members computer skills that would enable them to use the available computer equipment more efficiently. Using this module, each trainee designed a file to be used for the…

Culturally Relevant Human Subjects Protection Training: A Case Study in Community-Engaged Research in the United States.

PubMed

Kue, Jennifer; Szalacha, Laura A; Happ, Mary Beth; Crisp, Abigail L; Menon, Usha

2018-02-01

Non-academic members of research teams, such as community members, can perceive traditional human subjects protection training as lacking in cultural relevance. We present a case exemplar of the development of a human subjects protection training for research staff with limited English proficiency and/or no or limited research experience. Seven modules were adapted for language, cultural examples, etc., from the standard Collaborative Institutional Training Initiative (CITI) human subjects protection training. Non-academic research staff completed a day-long training in human subjects protection (six modules) and our research protocol (one module). We assessed comprehension of content with PowerPoint slides and module quizzes. All participants successfully passed each module quiz with ≥ 80% correct. Questions answered incorrectly were discussed before proceeding to the next module. To meet the increasing demand for collaborative community-engaged research with underserved minority populations, human subjects protection training protocols can be adapted successfully to reflect real-world situations and provide culturally relevant materials to help non-academic research staff better understand the importance and necessity of research ethics.

Linear Look-Ahead in Conjunctive Cells: An Entorhinal Mechanism for Vector-Based Navigation

PubMed Central

Kubie, John L.; Fenton, André A.

2012-01-01

The crisp organization of the “firing bumps” of entorhinal grid cells and conjunctive cells leads to the notion that the entorhinal cortex may compute linear navigation routes. Specifically, we propose a process, termed “linear look-ahead,” by which a stationary animal could compute a series of locations in the direction it is facing. We speculate that this computation could be achieved through learned patterns of connection strengths among entorhinal neurons. This paper has three sections. First, we describe the minimal grid cell properties that will be built into our network. Specifically, the network relies on “rigid modules” of neurons, where all members have identical grid scale and orientation, but differ in spatial phase. Additionally, these neurons must be densely interconnected with synapses that are modifiable early in the animal’s life. Second, we investigate whether plasticity during short bouts of locomotion could induce patterns of connections amongst grid cells or conjunctive cells. Finally, we run a simulation to test whether the learned connection patterns can exhibit linear look-ahead. Our results are straightforward. A simulated 30-min walk produces weak strengthening of synapses between grid cells that do not support linear look-ahead. Similar training in a conjunctive cell module produces a small subset of very strong connections between cells. These strong pairs have three properties: the pre- and post-synaptic cells have similar heading direction. The cell pairs have neighboring grid bumps. Finally, the spatial offset of firing bumps of the cell pair is in the direction of the common heading preference. Such a module can produce strong and accurate linear look-ahead starting in any location and extending in any direction. We speculate that this process may: (1) compute linear paths to goals; (2) update grid cell firing during navigation; and (3) stabilize the rigid modules of grid cells and conjunctive cells. PMID:22557948

STS-98 crew members take part in CEIT

NASA Technical Reports Server (NTRS)

2000-01-01

Inside the U.S. Lab, Destiny, members of the STS-98 crew work with technicians (in the background) to learn more about the equipment in the module. They are taking part in Crew Equipment Interface Test activities. At left, back to camera, is Mission Specialist Marsha Ivins. Standing are Mission Specialists Thomas Jones (left) and Robert Curbeam (right). Other crew members not seen are Commander Ken Cockrell and Pilot Mark Polansky. The mission will be transporting the Lab to the International Space Station with five system racks already installed inside of the module. With delivery of electronics in the lab, electrically powered attitude control for Control Moment Gyroscopes will be activated. The STS-98 launch is scheduled for Jan. 18, 2001.

Modulation of Tight Junction Structure and Function by Kinases and Phosphatases Targeting Occludin

PubMed Central

Dörfel, Max Johannes; Huber, Otmar

2012-01-01

Tight junctions (TJs) typically represent the most apical contacts in epithelial and endothelial cell layers where they play an essential role in the separation of extracellular or luminal spaces from underlying tissues in the body. Depending on the protein composition, TJs define the barrier characteristics and in addition maintain cell polarity. Two major families of integral membrane proteins form the typical TJ strand network, the tight junction-associated MARVEL protein (TAMP) family members occludin, tricellulin, and MarvelD3 as well as a specific set of claudins. Occludin was the first identified member of these tetraspanins and is now widely accepted as a regulator of TJ assembly and function. Therefore, occludin itself has to be tightly regulated. Phosphorylation of occludin appears to be of central importance in this context. Here we want to summarize current knowledge on the kinases and phosphatases directly modifying occludin, and their role in the regulation of TJ structure, function, and dynamics. PMID:22315516

KENNEDY SPACE CENTER, FLA. - Members of the STS-114 crew take a look at the Japanese Experiment Module (JEM) pressure module in the Space Station Processing Facility. A research laboratory, the pressurized module is the first element of the JEM, named "Kibo" (Hope), to be delivered to KSC. The National Space Development Agency of Japan (NASDA) developed the laboratory at the Tsukuba Space Center near Tokyo and is Japan's primary contribution to the Station. The JEM also includes an exposed facility (platform) for space environment experiments, a robotic manipulator system, and two logistics modules. The various JEM components will be assembled in space over the course of three Shuttle missions.

NASA Image and Video Library

2003-06-09

KENNEDY SPACE CENTER, FLA. - Members of the STS-114 crew take a look at the Japanese Experiment Module (JEM) pressure module in the Space Station Processing Facility. A research laboratory, the pressurized module is the first element of the JEM, named "Kibo" (Hope), to be delivered to KSC. The National Space Development Agency of Japan (NASDA) developed the laboratory at the Tsukuba Space Center near Tokyo and is Japan's primary contribution to the Station. The JEM also includes an exposed facility (platform) for space environment experiments, a robotic manipulator system, and two logistics modules. The various JEM components will be assembled in space over the course of three Shuttle missions.

Striking Similarity in the Gene Expression Levels of Individual Myc Module Members among ESCs, EpiSCs, and Partial iPSCs

PubMed Central

Hirasaki, Masataka; Hiraki-Kamon, Keiko; Kamon, Masayoshi; Suzuki, Ayumu; Katano, Miyuki; Nishimoto, Masazumi; Okuda, Akihiko

2013-01-01

Predominant transcriptional subnetworks called Core, Myc, and PRC modules have been shown to participate in preservation of the pluripotency and self-renewality of embryonic stem cells (ESCs). Epiblast stem cells (EpiSCs) are another cell type that possesses pluripotency and self-renewality. However, the roles of these modules in EpiSCs have not been systematically examined to date. Here, we compared the average expression levels of Core, Myc, and PRC module genes between ESCs and EpiSCs. EpiSCs showed substantially higher and lower expression levels of PRC and Core module genes, respectively, compared with those in ESCs, while Myc module members showed almost equivalent levels of average gene expression. Subsequent analyses revealed that the similarity in gene expression levels of the Myc module between these two cell types was not just overall, but striking similarities were evident even when comparing the expression of individual genes. We also observed equivalent levels of similarity in the expression of individual Myc module genes between induced pluripotent stem cells (iPSCs) and partial iPSCs that are an unwanted byproduct generated during iPSC induction. Moreover, our data demonstrate that partial iPSCs depend on a high level of c-Myc expression for their self-renewal properties. PMID:24386274

Sexy DEG/ENaC channels involved in gustatory detection of fruit fly pheromones.

PubMed

Pikielny, Claudio W

2012-11-06

Hydrocarbon pheromones on the cuticle of Drosophila melanogaster modulate the complex courtship behavior of males. Recently, three members of the degenerin/epithelial Na+ channel (DEG/ENaC) family of sodium channel subunits, Ppk25, Ppk23, and Ppk29 (also known as Nope), have been shown to function in gustatory perception of courtship-modulating contact pheromones. All three proteins are required for the activation of male courtship by female pheromones. Specific interactions between two of them have been demonstrated in cultured cells, suggesting that, in a subset of cells where they are coexpressed, these three subunits function within a common heterotrimeric DEG/ENaC channel. Such a DEG/ENaC channel may be gated by pheromones, either directly or indirectly, or alternatively may control the excitability of pheromone-sensing cells. In addition, these studies identify taste neurons that respond specifically to courtship-modulating pheromones and mediate their effects on male behavior. Two types of pheromone-sensing taste neurons, F and M cells, have been defined on the basis of their specific response to either female or male pheromones. These reports set the stage for the dissection of the molecular and cellular mechanisms that mediate gustatory detection of contact pheromones.

Conformational analysis of the Streptococcus pneumoniae hyaluronate lyase and characterization of its hyaluronan-specific carbohydrate-binding module.

PubMed

Suits, Michael D L; Pluvinage, Benjamin; Law, Adrienne; Liu, Yan; Palma, Angelina S; Chai, Wengang; Feizi, Ten; Boraston, Alisdair B

2014-09-26

For a subset of pathogenic microorganisms, including Streptococcus pneumoniae, the recognition and degradation of host hyaluronan contributes to bacterial spreading through the extracellular matrix and enhancing access to host cell surfaces. The hyaluronate lyase (Hyl) presented on the surface of S. pneumoniae performs this role. Using glycan microarray screening, affinity electrophoresis, and isothermal titration calorimetry we show that the N-terminal module of Hyl is a hyaluronan-specific carbohydrate-binding module (CBM) and the founding member of CBM family 70. The 1.2 Å resolution x-ray crystal structure of CBM70 revealed it to have a β-sandwich fold, similar to other CBMs. The electrostatic properties of the binding site, which was identified by site-directed mutagenesis, are distinct from other CBMs and complementary to its acidic ligand, hyaluronan. Dynamic light scattering and solution small angle x-ray scattering revealed the full-length Hyl protein to exist as a monomer/dimer mixture in solution. Through a detailed analysis of the small angle x-ray scattering data, we report the pseudoatomic solution structures of the monomer and dimer forms of the full-length multimodular Hyl. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

75 FR 78794 - Self-Regulatory Organizations; EDGX Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-16

... (Direct) \\4\\ or through the ECN Translator.\\5\\ Members and non-members will receive the first ten (10... sessions will not apply to ECN Translator sessions to incent members and non-members to use Direct Sessions... the Exchange's secondary data center. \\5\\ The ECN translator allows a Member or non-member who...

75 FR 78791 - Self-Regulatory Organizations; EDGA Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-16

... (Direct) \\4\\ or through the ECN Translator.\\5\\ Members and non-members will receive the first ten (10... sessions will not apply to ECN Translator sessions to incent members and non-members to use Direct Sessions... the Exchange's secondary data center. \\5\\ The ECN translator allows a Member or non-member who...

Pilot study of traumatic brain injury and alcohol misuse among service members.

PubMed

Bogner, Jennifer; French, Louis M; Lange, Rael T; Corrigan, John D

2015-01-01

Explore relationships among traumatic brain injury (TBI), substance misuse and other mental health disorders in US service members and to identify risk factors for substance misuse. Service members (n = 93 in final sample) injured while deployed to Operation Enduring Freedom or Operation Iraqi Freedom. Longitudinal survey at 6 and 12 months post-intake. The following measures were used: problem substance use, Alcohol Expectancies Questionnaire-III, MINI International Neuropsychiatric Interview Substance Abuse Modules, Ohio State University TBI Identification Method, Neurobehavioural Symptom Inventory, Rivermead Post-Concussion Symptoms Questionnaire, Buss-Perry Aggression Questionnaire, Post-Traumatic Stress Disorder Checklist-Civilian Version, Beck Depression Inventory-II, Beck Anxiety Inventory. More severe TBI and post-traumatic stress disorder (PTSD) symptoms at 6 months post-enrolment were associated with decreased odds of substance misuse 12 months after study enrolment. Alcohol expectancies and incurring a TBI at a younger age increased the odds of substance misuse. While the ability to generalize the current findings to a larger population is limited, the results provide direction for future studies on the prevention and treatment of substance misuse following TBI. The unexpected protective effect of more severe TBI may result from prospective attention to the injury and its consequences. Greater preventive benefit may result from identifying more service members with elevated risk. Lifetime history of TBI and alcohol expectancies may be candidate indicators for greater attention.

Module Cluster: RTE-001.00 (GSC) Advanced Teaching of Reading.

ERIC Educational Resources Information Center

Brown, Estelle

Several module clusters were developed at Glassboro State College as the result of involvement in the Camden Teacher Corps project. The clusters are the primary mode of instruction in this competency-based teacher education program. Many of these modules are based on a list of teacher competencies developed by members of the Elementary Education…

Regulation of RB Transcription In Vivo by RB Family Members▿ ‡

PubMed Central

Burkhart, Deborah L.; Ngai, Lynn K.; Roake, Caitlin M.; Viatour, Patrick; Thangavel, Chellappagounder; Ho, Victoria M.; Knudsen, Erik S.; Sage, Julien

2010-01-01

In cancer cells, the retinoblastoma tumor suppressor RB is directly inactivated by mutation in the RB gene or functionally inhibited by abnormal activation of cyclin-dependent kinase activity. While variations in RB levels may also provide an important means of controlling RB function in both normal and cancer cells, little is known about the mechanisms regulating RB transcription. Here we show that members of the RB and E2F families bind directly to the RB promoter. To investigate how the RB/E2F pathway may regulate Rb transcription, we generated reporter mice carrying an eGFP transgene inserted into a bacterial artificial chromosome containing most of the Rb gene. Expression of eGFP largely parallels that of Rb in transgenic embryos and adult mice. Using these reporter mice and mutant alleles for Rb, p107, and p130, we found that RB family members modulate Rb transcription in specific cell populations in vivo and in culture. Interestingly, while Rb is a target of the RB/E2F pathway in mouse and human cells, Rb expression does not strictly correlate with the cell cycle status of these cells. These experiments identify novel regulatory feedback mechanisms within the RB pathway in mammalian cells. PMID:20100864

Structure and Functional Characterization of the RNA-Binding Element of the NLRX1 Innate Immune Modulator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hong, Minsun; Yoon, Sung-il; Wilson, Ian A.

2012-06-20

Mitochondrial NLRX1 is a member of the family of nucleotide-binding domain and leucine-rich-repeat-containing proteins (NLRs) that mediate host innate immunity as intracellular surveillance sensors against common molecular patterns of invading pathogens. NLRX1 functions in antiviral immunity, but the molecular mechanism of its ligand-induced activation is largely unknown. The crystal structure of the C-terminal fragment (residues 629975) of human NLRX1 (cNLRX1) at 2.65 {angstrom} resolution reveals that cNLRX1 consists of an N-terminal helical (LRRNT) domain, central leucine-rich repeat modules (LRRM), and a C-terminal three-helix bundle (LRRCT). cNLRX1 assembles into a compact hexameric architecture that is stabilized by intersubunit and interdomain interactionsmore » of LRRNT and LRRCT in the trimer and dimer components of the hexamer, respectively. Furthermore, we find that cNLRX1 interacts directly with RNA and supports a role for NLRX1 in recognition of intracellular viral RNA in antiviral immunity.« less

A multi-layered mechanistic modelling approach to understand how effector genes extend beyond phytoplasma to modulate plant hosts, insect vectors and the environment.

PubMed

Tomkins, Melissa; Kliot, Adi; Marée, Athanasius Fm; Hogenhout, Saskia A

2018-03-13

Members of the Candidatus genus Phytoplasma are small bacterial pathogens that hijack their plant hosts via the secretion of virulence proteins (effectors) leading to a fascinating array of plant phenotypes, such as witch's brooms (stem proliferations) and phyllody (retrograde development of flowers into vegetative tissues). Phytoplasma depend on insect vectors for transmission, and interestingly, these insect vectors were found to be (in)directly attracted to plants with these phenotypes. Therefore, phytoplasma effectors appear to reprogram plant development and defence to lure insect vectors, similarly to social engineering malware, which employs tricks to lure people to infected computers and webpages. A multi-layered mechanistic modelling approach will enable a better understanding of how phytoplasma effector-mediated modulations of plant host development and insect vector behaviour contribute to phytoplasma spread, and ultimately to predict the long reach of phytoplasma effector genes. Copyright © 2018. Published by Elsevier Ltd.

Cell intrinsic modulation of Wnt signaling controls neuroblast migration in C. elegans.

PubMed

Mentink, Remco A; Middelkoop, Teije C; Rella, Lorenzo; Ji, Ni; Tang, Chung Yin; Betist, Marco C; van Oudenaarden, Alexander; Korswagen, Hendrik C

2014-10-27

Members of the Wnt family of secreted signaling proteins are key regulators of cell migration and axon guidance. In the nematode C. elegans, the migration of the QR neuroblast descendants requires multiple Wnt ligands and receptors. We found that the migration of the QR descendants is divided into three sequential phases that are each mediated by a distinct Wnt signaling mechanism. Importantly, the transition from the first to the second phase, which is the main determinant of the final position of the QR descendants along the anteroposterior body axis, is mediated through a cell-autonomous process in which the time-dependent expression of a Wnt receptor turns on the canonical Wnt/β-catenin signaling response that is required to terminate long-range anterior migration. Our results show that, in addition to direct guidance of cell migration by Wnt morphogenic gradients, cell migration can also be controlled indirectly through cell-intrinsic modulation of Wnt signaling responses.

Chirped laser dispersion spectroscopy using a directly modulated quantum cascade laser

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hangauer, Andreas, E-mail: hangauer@princeton.edu; Nikodem, Michal; Wysocki, Gerard, E-mail: gwysocki@princeton.edu

2013-11-04

Chirped laser dispersion spectroscopy (CLaDS) utilizing direct modulation of a quantum cascade laser (QCL) is presented. By controlling the laser bias nearly single- and dual-sideband CLaDS operation can be realized in an extremely simplified optical setup with no external optical modulators. Capability of direct single-sideband modulation is a unique feature of QCLs that exhibit a low linewidth enhancement factor. The developed analytical model shows excellent agreement with the experimental, directly modulated CLaDS spectra. This method overcomes major technical limitations of mid-infrared CLaDS systems by allowing significantly higher modulation frequencies and eliminating optical fringes introduced by external modulators.

The MADS transcription factor XAL2/AGL14 modulates auxin transport during Arabidopsis root development by regulating PIN expression

PubMed Central

Garay-Arroyo, Adriana; Ortiz-Moreno, Enrique; de la Paz Sánchez, María; Murphy, Angus S; García-Ponce, Berenice; Marsch-Martínez, Nayelli; de Folter, Stefan; Corvera-Poiré, Adriana; Jaimes-Miranda, Fabiola; Pacheco-Escobedo, Mario A; Dubrovsky, Joseph G; Pelaz, Soraya; Álvarez-Buylla, Elena R

2013-01-01

Elucidating molecular links between cell-fate regulatory networks and dynamic patterning modules is a key for understanding development. Auxin is important for plant patterning, particularly in roots, where it establishes positional information for cell-fate decisions. PIN genes encode plasma membrane proteins that serve as auxin efflux transporters; mutations in members of this gene family exhibit smaller roots with altered root meristems and stem-cell patterning. Direct regulators of PIN transcription have remained elusive. Here, we establish that a MADS-box gene (XAANTAL2, XAL2/AGL14) controls auxin transport via PIN transcriptional regulation during Arabidopsis root development; mutations in this gene exhibit altered stem-cell patterning, root meristem size, and root growth. XAL2 is necessary for normal shootward and rootward auxin transport, as well as for maintaining normal auxin distribution within the root. Furthermore, this MADS-domain transcription factor upregulates PIN1 and PIN4 by direct binding to regulatory regions and it is required for PIN4-dependent auxin response. In turn, XAL2 expression is regulated by auxin levels thus establishing a positive feedback loop between auxin levels and PIN regulation that is likely to be important for robust root patterning. PMID:24121311

SH2 domains: modulators of nonreceptor tyrosine kinase activity.

PubMed

Filippakopoulos, Panagis; Müller, Susanne; Knapp, Stefan

2009-12-01

The Src homology 2 (SH2) domain is a sequence-specific phosphotyrosine-binding module present in many signaling molecules. In cytoplasmic tyrosine kinases, the SH2 domain is located N-terminally to the catalytic kinase domain (SH1) where it mediates cellular localization, substrate recruitment, and regulation of kinase activity. Initially, structural studies established a role of the SH2 domain stabilizing the inactive state of Src family members. However, biochemical characterization showed that the presence of the SH2 domain is frequently required for catalytic activity, suggesting a crucial function stabilizing the active state of many nonreceptor tyrosine kinases. Recently, the structure of the SH2-kinase domain of Fes revealed that the SH2 domain stabilizes the active kinase conformation by direct interactions with the regulatory helix alphaC. Stabilizing interactions between the SH2 and the kinase domains have also been observed in the structures of active Csk and Abl. Interestingly, mutations in the SH2 domain found in human disease can be explained by SH2 domain destabilization or incorrect positioning of the SH2. Here we summarize our understanding of mechanisms that lead to tyrosine kinase activation by direct interactions mediated by the SH2 domain and discuss how mutations in the SH2 domain trigger kinase inactivation.

Oxytocin modulates the racial bias in neural responses to others' suffering.

PubMed

Sheng, Feng; Liu, Yi; Zhou, Bin; Zhou, Wen; Han, Shihui

2013-02-01

The intergroup relationship between a perceiver and a target person influences empathic neural responses to others' suffering, which are increased for racial in-group members compared to out-group members. The current study investigated whether oxytocin (OT), a neuropeptide that has been linked to empathic concern and in-group favoritism, contributes to the racial bias in empathic neural responses. Event-related brain potentials were recorded in Chinese male adults during race judgments on Asian and Caucasian faces expressing pain or showing a neutral expression after intranasal self-administration of OT or placebo. A fronto-central positive activity at 128-188 ms (P2) was of larger amplitude in response to the pain expressions compared with the neutral expressions of racial in-group members but not of racial out-group members. OT treatment increased this racial in-group bias in neural responses and resulted in its correlation with a positive implicit attitude toward racial in-group members. Our findings suggest that OT interacts with the intergroup relationship to modulate empathic neural responses to others' suffering. Copyright © 2012 Elsevier B.V. All rights reserved.

Directed network modules

NASA Astrophysics Data System (ADS)

Palla, Gergely; Farkas, Illés J.; Pollner, Péter; Derényi, Imre; Vicsek, Tamás

2007-06-01

A search technique locating network modules, i.e. internally densely connected groups of nodes in directed networks is introduced by extending the clique percolation method originally proposed for undirected networks. After giving a suitable definition for directed modules we investigate their percolation transition in the Erdos-Rényi graph both analytically and numerically. We also analyse four real-world directed networks, including Google's own web-pages, an email network, a word association graph and the transcriptional regulatory network of the yeast Saccharomyces cerevisiae. The obtained directed modules are validated by additional information available for the nodes. We find that directed modules of real-world graphs inherently overlap and the investigated networks can be classified into two major groups in terms of the overlaps between the modules. Accordingly, in the word-association network and Google's web-pages, overlaps are likely to contain in-hubs, whereas the modules in the email and transcriptional regulatory network tend to overlap via out-hubs.

Cable tensioned membrane solar collector module with variable tension control

DOEpatents

Murphy, L.M.

1984-01-09

Disclosed is a solar collector comprising a membrane member for concentrating sunlight, a plurality of elongated structural members for suspending the membrane member thereon, and a plurality of control members for adjustably tensioning the membrane member, as well as for controlling a focus produced by the membrane members. Each control member is disposed at a different corresponding one of the plurality of structural members. The collector also comprises an elongated flexible tensioning member, which serves to stretch the membrane member and to thereafter hold it in tension, and a plurality of sleeve members which serve to provide the membrane member with a desired surface contour during tensioning of the membrane member. The tensioning member is coupled to the structural members such that the tensioning member is adjustably tensioned through the structural members. The tensioning member is also coupled to the membrane member through the sleeve members such that the sleeve members uniformly and symmetrically stretch the membrane member upon applying tension to the tensioning member with the control members.

Defining quality criteria for online continuing medical education modules using modified nominal group technique.

PubMed

Shortt, S E D; Guillemette, Jean-Marc; Duncan, Anne Marie; Kirby, Frances

2010-01-01

The rapid increase in the use of the Internet for continuing education by physicians suggests the need to define quality criteria for accredited online modules. Continuing medical education (CME) directors from Canadian medical schools and academic researchers participated in a consensus process, Modified Nominal Group Technique, to develop agreement on the most important quality criteria to guide module development. Rankings were compared to responses to a survey of a subset of Canadian Medical Association (CMA) members. A list of 17 items was developed, of which 10 were deemed by experts to be important and 7 were considered secondary. A quality module would: be needs-based; presented in a clinical format; utilize evidence-based information; permit interaction with content and experts; facilitate and attempt to document practice change; be accessible for later review; and include a robust course evaluation. There was less agreement among CMA members on criteria ranking, with consensus on ranking reached on only 12 of 17 items. In contrast to experts, members agreed that the need to assess performance change as a result of an educational experience was not important. This project identified 10 quality criteria for accredited online CME modules that representatives of Canadian organizations involved in continuing education believe should be taken into account when developing learning products. The lack of practitioner support for documentation of change in clinical behavior may suggest that they favor traditional attendance- or completion-based CME; this finding requires further research.

Cable tensioned membrane solar collector module with variable tension control

DOEpatents

Murphy, Lawrence M.

1985-01-01

Disclosed is a solar collector comprising a membrane for concentrating sunlight, a plurality of elongated structural members for suspending the membrane member thereon, and a plurality of control members for adjustably tensioning the membrane member, as well as for controlling a focus produced by the membrane members. Each control member is disposed at a different corresponding one of the plurality of structural members. The collector also comprises an elongated flexible tensioning member, which serves to stretch the membrane member and to thereafter hold it in tension, and a plurality of sleeve members, which serve to provide the membrane member with a desired surface contour during tensioning of the membrane member. The tensioning member is coupled to the structural members such that the tensioning member is adjustably tensioned through the structural members. The tensioning member is also coupled to the membrane member through the sleeve members such that the sleeve members uniformly and symmetrically stretch the membrane member upon applying tension to the tensioning member with the control members.

Molecular mechanics and dynamics characterization of an in silico mutated protein: a stand-alone lab module or support activity for in vivo and in vitro analyses of targeted proteins.

PubMed

Chiang, Harry; Robinson, Lucy C; Brame, Cynthia J; Messina, Troy C

2013-01-01

Over the past 20 years, the biological sciences have increasingly incorporated chemistry, physics, computer science, and mathematics to aid in the development and use of mathematical models. Such combined approaches have been used to address problems from protein structure-function relationships to the workings of complex biological systems. Computer simulations of molecular events can now be accomplished quickly and with standard computer technology. Also, simulation software is freely available for most computing platforms, and online support for the novice user is ample. We have therefore created a molecular dynamics laboratory module to enhance undergraduate student understanding of molecular events underlying organismal phenotype. This module builds on a previously described project in which students use site-directed mutagenesis to investigate functions of conserved sequence features in members of a eukaryotic protein kinase family. In this report, we detail the laboratory activities of a MD module that provide a complement to phenotypic outcomes by providing a hypothesis-driven and quantifiable measure of predicted structural changes caused by targeted mutations. We also present examples of analyses students may perform. These laboratory activities can be integrated with genetics or biochemistry experiments as described, but could also be used independently in any course that would benefit from a quantitative approach to protein structure-function relationships. Copyright © 2013 Wiley Periodicals, Inc.

Astronauts Working in Spacelab

NASA Technical Reports Server (NTRS)

1999-01-01

This Quick Time movie captures astronaut Jan Davis and her fellow crew members working in the Spacelab, a versatile laboratory carried in the Space Shuttle's cargo bay for special research flights. Its various elements can be combined to accommodate the many types of scientific research that can best be performed in space. Spacelab consisted of an enclosed, pressurized laboratory module and open U-shaped pallets located at the rear of the laboratory module. The laboratory module contained utilities, computers, work benches, and instrument racks to conduct scientific experiments in astronomy, physics, chemistry, biology, medicine, and engineering. Equipment, such as telescopes, antennas, and sensors, is mounted on pallets for direct exposure to space. A 1-meter (3.3-ft.) diameter aluminum tunnel, resembling a z-shaped tube, connected the crew compartment (mid deck) to the module. The reusable Spacelab allowed scientists to bring experiment samples back to Earth for post-flight analysis. Spacelab was a cooperative venture of the European Space Agency (ESA) and NASA. ESA was responsible for funding, developing, and building Spacelab, while NASA was responsible for the launch and operational use of Spacelab. Spacelab missions were cooperative efforts between scientists and engineers from around the world. Teams from NASA centers, universities, private industry, government agencies and international space organizations designed the experiments. The Marshall Space Flight Center was NASA's lead center for monitoring the development of Spacelab and managing the program.

Integrated manufacturing approach to attain benchmark team performance

NASA Astrophysics Data System (ADS)

Chen, Shau-Ron; Nguyen, Andrew; Naguib, Hussein

1994-09-01

A Self-Directed Work Team (SDWT) was developed to transfer a polyimide process module from the research laboratory to our wafer fab facility for applications in IC specialty devices. The SDWT implemented processes and tools based on the integration of five manufacturing strategies for continuous improvement. These were: Leadership Through Quality (LTQ), Total Productive Maintenance (TMP), Cycle Time Management (CTM), Activity-Based Costing (ABC), and Total Employee Involvement (TEI). Utilizing these management techniques simultaneously, the team achieved six sigma control of all critical parameters, increased Overall Equipment Effectiveness (OEE) from 20% to 90%, reduced cycle time by 95%, cut polyimide manufacturing cost by 70%, and improved its overall team member skill level by 33%.

KSC-07pd2843

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, members of the STS-123 crew learn more about the mission payload, the Kibo Experiment Logistics Module Pressurized Section. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

Inhibiting NANOG Enhances Efficacy of BH3 Mimetics | Center for Cancer Research

Cancer.gov

BCL-2 family proteins regulate cell fate. Some members promote cell survival while others induce programmed cell death. A third group, the BH3-only members, modulates the activities of the rest of the family. Some cancers, including those of the colon and rectum, express elevated levels of pro-survival BCL-2 members, which may protect cancer cells from chemotherapy. BH3

Traumatic Brain Injury: A Guide for Caregivers of Service Members and Veterans. Welcome

DTIC Science & Technology

2010-04-01

Reed Army Medical Center, 33 percent had traumatic brain injury (as of March 2009). Due to medical and technological advances, we have seen a...Right to Appeal PEB Decision - Module 4, pages 139-141 PEG Tube (Per Cutaneous Gastronomy Tube) - Module 1, page 23 PRC (VA Polytrauma Rehabilitation...Per Cutaneous Gastronomy Tube (PEG tube) - Module 1, page 23 Peripheral Fatigue - Module 2, page 17 IN D EX 29 Permanent Disability Retired List (PDRL

Engineering study of the module/array interface for large terrestrial photovoltaic arrays

NASA Technical Reports Server (NTRS)

1977-01-01

Three major areas--structural, electrical, and maintenance--were evaluated. Efforts in the structural area included establishing acceptance criteria for materials and members, determining loading criteria, and analyzing glass modules in various framing system configurations. Array support structure design was addressed briefly. Electrical considerations included evaluation of module characteristics, intermodule connectors, array wiring, converters and lightning protection. Plant maintenance features such as array cleaning, failure detection, and module installation and replacement were addressed.

View of damaged Apollo 13 Service Module from the Lunar/Command Modules

NASA Image and Video Library

1970-04-17

AS13-59-8562 (17 April 1970) --- This view of the Apollo 13 Lunar Module (LM) was photographed from the Command Module (CM) just after the LM had been jettisoned. The jettisoning occurred a few minutes before 11 a.m. (CST), April 17, 1970, just over an hour prior to splashdown of the CM in the south Pacific Ocean. The apparent explosion of oxygen tank number two in the Apollo 13 Service Module (SM) caused the Apollo 13 crew members to rely on the LM as a "lifeboat".

Acoustic imaging microscope

DOEpatents

Deason, Vance A.; Telschow, Kenneth L.

2006-10-17

An imaging system includes: an object wavefront source and an optical microscope objective all positioned to direct an object wavefront onto an area of a vibrating subject surface encompassed by a field of view of the microscope objective, and to direct a modulated object wavefront reflected from the encompassed surface area through a photorefractive material; and a reference wavefront source and at least one phase modulator all positioned to direct a reference wavefront through the phase modulator and to direct a modulated reference wavefront from the phase modulator through the photorefractive material to interfere with the modulated object wavefront. The photorefractive material has a composition and a position such that interference of the modulated object wavefront and modulated reference wavefront occurs within the photorefractive material, providing a full-field, real-time image signal of the encompassed surface area.

Calibration-free absolute frequency response measurement of directly modulated lasers based on additional modulation.

PubMed

Zhang, Shangjian; Zou, Xinhai; Wang, Heng; Zhang, Yali; Lu, Rongguo; Liu, Yong

2015-10-15

A calibration-free electrical method is proposed for measuring the absolute frequency response of directly modulated semiconductor lasers based on additional modulation. The method achieves the electrical domain measurement of the modulation index of directly modulated lasers without the need for correcting the responsivity fluctuation in the photodetection. Moreover, it doubles measuring frequency range by setting a specific frequency relationship between the direct and additional modulation. Both the absolute and relative frequency response of semiconductor lasers are experimentally measured from the electrical spectrum of the twice-modulated optical signal, and the measured results are compared to those obtained with conventional methods to check the consistency. The proposed method provides calibration-free and accurate measurement for high-speed semiconductor lasers with high-resolution electrical spectrum analysis.

Frequency response control of semiconductor laser by using hybrid modulation scheme.

PubMed

Mieda, Shigeru; Yokota, Nobuhide; Isshiki, Ryuto; Kobayashi, Wataru; Yasaka, Hiroshi

2016-10-31

A hybrid modulation scheme that simultaneously applies the direct current modulation and intra-cavity loss modulation to a semiconductor laser is proposed. Both numerical calculations using rate equations and experiments using a fabricated laser show that the hybrid modulation scheme can control the frequency response of the laser by changing a modulation ratio and time delay between the two modulations. The modulation ratio and time delay provide the degree of signal mixing of the two modulations and an optimum condition is found when a non-flat frequency response for the intra-cavity loss modulation is compensated by that for the direct current modulation. We experimentally confirm a 8.64-dB improvement of the modulation sensitivity at 20 GHz compared with the pure direct current modulation with a 0.7-dB relaxation oscillation peak.

Breaking Down Difficult Family Patterns. Fostering Families. A Specialized Training Program Designed for Foster Care Workers & Foster Care Parents.

ERIC Educational Resources Information Center

Schatz, Mona Struhsaker; And Others

This module is part of a training program for foster parents and foster care workers offered at Colorado State University. The module examines the functioning of families with addictive and dependent members. The module's learning objectives address: (1) indicators of addiction problems in families, and cycles of substance use and abuse; (2) roles…

Professional Teacher Education Module Series. Assist Student Vocational Organization Members in Developing and Financing a Yearly Program of Activities, Module H-4 of Category H--Student Vocational Organization.

ERIC Educational Resources Information Center

Ohio State Univ., Columbus. National Center for Research in Vocational Education.

This fourth in a series of six learning modules on student vocational organizations is designed to assist secondary and postsecondary vocational teachers in developing the competency to assist students in planning a student organization's (or club's) yearly program of activities, in properly managing organization finances, in selecting…

Mangroves Build Land. "Mangroves are a Valuable Resource." Grades 7 and 8. A Two Lesson Unit. Student Learning Activity Module.

ERIC Educational Resources Information Center

Frank, James

This module is an activity and film-oriented unit focusing on the importance of mangroves in the South Florida ecosystem. The module is part of a series designed to be used by teachers, students, and community members to help them utilize community resources in developing and teaching environmental concepts and responsibility, and in seeking ways…

Akt regulates drug-induced cell death through Bcl-w downregulation.

PubMed

Garofalo, Michela; Quintavalle, Cristina; Zanca, Ciro; De Rienzo, Assunta; Romano, Giulia; Acunzo, Mario; Puca, Loredana; Incoronato, Mariarosaria; Croce, Carlo M; Condorelli, Gerolama

2008-01-01

Akt is a serine threonine kinase with a major role in transducing survival signals and regulating proteins involved in apoptosis. To find new interactors of Akt involved in cell survival, we performed a two-hybrid screening in yeast using human full-length Akt c-DNA as bait and a murine c-DNA library as prey. Among the 80 clones obtained, two were identified as Bcl-w. Bcl-w is a member of the Bcl-2 family that is essential for the regulation of cellular survival, and that is up-regulated in different human tumors, such as gastric and colorectal carcinomas. Direct interaction of Bcl-w with Akt was confirmed by immunoprecipitation assays. Subsequently, we addressed the function of this interaction: by interfering with the activity or amount of Akt, we have demonstrated that Akt modulates the amount of Bcl-w protein. We have found that inhibition of Akt activity may promote apoptosis through the downregulation of Bcl-w protein and the consequential reduction in interaction of Bcl-w with pro-apoptotic members of the Bcl-2 family. Our data provide evidence that Bcl-w is a new member of the Akt pathway and that Akt may induce anti-apoptotic signals at least in part through the regulation of the amount and activity of Bcl-w.

12 CFR 211.8 - Investments and activities abroad.

Code of Federal Regulations, 2010 CFR

2010-01-01

... of a member bank is deemed to be an investor. (b) Direct investments by member banks. A member bank's direct investments under section 25 of the FRA (12 U.S.C. 601 et seq.) shall be limited to: (1) Foreign... investments in an organization, provided that: (i) Individual investment limits. The total direct and indirect...

The payload canister leaves the O&C with the Joint Airlock Module inside

NASA Technical Reports Server (NTRS)

2000-01-01

The payload canister, with the Joint Airlock Module inside, backs out of the Operations and Checkout Building for a short trip to the Space Station Processing Facility. There the module will undergo more preflight processing for the STS-104 mission scheduled for launch aboard Space Shuttle Atlantis May 17, 2001. The Joint Airlock Module is the gateway from which crew members aboard the International Space Station will enter and exit the 470-ton orbiting research facility.

Glucose and auxin signaling interaction in controlling Arabidopsis thaliana seedlings root growth and development.

PubMed

Mishra, Bhuwaneshwar S; Singh, Manjul; Aggrawal, Priyanka; Laxmi, Ashverya

2009-01-01

Plant root growth and development is highly plastic and can adapt to many environmental conditions. Sugar signaling has been shown to affect root growth and development by interacting with phytohormones such as gibberellins, cytokinin and abscisic acid. Auxin signaling and transport has been earlier shown to be controlling plant root length, number of lateral roots, root hair and root growth direction. Increasing concentration of glucose not only controls root length, root hair and number of lateral roots but can also modulate root growth direction. Since root growth and development is also controlled by auxin, whole genome transcript profiling was done to find out the extent of interaction between glucose and auxin response pathways. Glucose alone could transcriptionally regulate 376 (62%) genes out of 604 genes affected by IAA. Presence of glucose could also modulate the extent of regulation 2 fold or more of almost 63% genes induced or repressed by IAA. Interestingly, glucose could affect induction or repression of IAA affected genes (35%) even if glucose alone had no significant effect on the transcription of these genes itself. Glucose could affect auxin biosynthetic YUCCA genes family members, auxin transporter PIN proteins, receptor TIR1 and members of a number of gene families including AUX/IAA, GH3 and SAUR involved in auxin signaling. Arabidopsis auxin receptor tir1 and response mutants, axr2, axr3 and slr1 not only display a defect in glucose induced change in root length, root hair elongation and lateral root production but also accentuate glucose induced increase in root growth randomization from vertical suggesting glucose effects on plant root growth and development are mediated by auxin signaling components. Our findings implicate an important role of the glucose interacting with auxin signaling and transport machinery to control seedling root growth and development in changing nutrient conditions.

Ultra high frequency imaging acoustic microscope

DOEpatents

Deason, Vance A.; Telschow, Kenneth L.

2006-05-23

An imaging system includes: an object wavefront source and an optical microscope objective all positioned to direct an object wavefront onto an area of a vibrating subject surface encompassed by a field of view of the microscope objective, and to direct a modulated object wavefront reflected from the encompassed surface area through a photorefractive material; and a reference wavefront source and at least one phase modulator all positioned to direct a reference wavefront through the phase modulator and to direct a modulated reference wavefront from the phase modulator through the photorefractive material to interfere with the modulated object wavefront. The photorefractive material has a composition and a position such that interference of the modulated object wavefront and modulated reference wavefront occurs within the photorefractive material, providing a full-field, real-time image signal of the encompassed surface area.

A Scalable Web-Based Module for Improving Surgical and Medical Practitioner Knowledge and Attitudes about Palliative and End-of-Life Care.

PubMed

Bergman, Jonathan; Lorenz, Karl A; Ballon-Landa, Eric; Kwan, Lorna; Lerman, Steven E; Saigal, Christopher S; Bennett, Carol J; Litwin, Mark S

2015-05-01

We built a web-based, interactive, self-directed learning module about end-of-life care. The study objective was to develop an online module about end-of-life care targeted at surgeons, and to assess the effect of the module on attitudes towards and knowledge about end-of-life care. Informed by a panel of experts in supportive care and educational assessment, we developed an instrument that required approximately 15 minutes to complete. The module targets surgeons, but is applicable to other practitioners as well. We recruited general surgeons, surgical subspecialists, and medical practitioners and subspecialists from UCLA and the GLA-VA (N=114). We compared pre- and post-intervention scores for attitude and knowledge, then used ANOVA to compare the pre- and postmodule means for each level of the covariate. We performed bivariable analyses to assess the association of subject characteristic and change in score over time. We ran separate analyses to assess baseline and change scores based on the covariates we had selected a priori. Subjects improved meaningfully in all five domains of attitude and in each of the six knowledge items. Individuals younger than 30 years of age had the greatest change in attitudes about addressing pain, addressing end-of-life goals, and being actively involved as death approached; they also had the most marked improvement in total knowledge score. Having a family member die of cancer within the last five years or a personal experience with palliative care or hospice were associated with higher change scores. A web-based education module improved surgical and medical provider attitudes and knowledge about end-of-life care.

Multifunction audio digitizer. [producing direct delta and pulse code modulation

NASA Technical Reports Server (NTRS)

Monford, L. G., Jr. (Inventor)

1974-01-01

An illustrative embodiment of the invention includes apparatus which simultaneously produces both direct delta modulation and pulse code modulation. An input signal, after amplification, is supplied to a window comparator which supplies a polarity control signal to gate the output of a clock to the appropriate input of a binary up-down counter. The control signals provide direct delta modulation while the up-down counter output provides pulse code modulation.

Module isolation devices

DOEpatents

Carolan, Michael Francis; Cooke, John Albert; Buzinski, Michael David

2010-04-27

A gas flow isolation device includes a gas flow isolation valve movable from an opened condition to a closed condition. The module isolation valve in one embodiment includes a rupture disk in flow communication with a flow of gas when the module isolation valve is in an opened condition. The rupture disk ruptures when a predetermined pressure differential occurs across it causing the isolation valve to close. In one embodiment the valve is mechanically linked to the rupture disk to maintain the valve in an opened condition when the rupture disk is intact, and which permits the valve to move into a closed condition when the rupture disk ruptures. In another embodiment a crushable member maintains the valve in an open condition, and the flow of gas passed the valve upon rupturing of the rupture disk compresses the crushable member to close the isolation valve.

KSC-99pp1488

NASA Image and Video Library

1999-12-09

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT), members of the STS-101 crew learn about some of the cargo that will be on their mission from workers at SPACEHAB, in Cape Canaveral, Fla. At left are Commander James Donald Halsell Jr., and Mission Specialist Mary Ellen Weber, (Ph.D.). Other crew members are Pilot Scott Horowitz, and Mission Specialists Edward Lu, Jeffrey N. Williams, and Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1487

NASA Image and Video Library

1999-12-09

During a Crew Equipment Interface Test (CEIT), members of the STS-101 crew learn about some of the cargo that will be on their mission from workers at SPACEHAB, in Cape Canaveral, Fla. At left are Mission Specialists Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. Other crew members are Commander James Donald Halsell Jr., Pilot Scott Horowitz, and Mission Specialists Mary Ellen Weber (Ph.D.), Edward Lu, and Jeffrey N. Williams, The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1490

NASA Image and Video Library

1999-12-09

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., members of the STS-101 crew learn how to manipulate the Russian crane Strela. At left is Yuri Malenchenko, who is with the Russian Space Agency (RSA); in the center is Edward Tsang Lu (Ph.D.); at right is Mission Specialist Jeffrey N. Williams. Other crew members are Commander James Donald Halsell Jr., Pilot Scott Horowitz, and Mission Specialists Mary Ellen Weber, (Ph.D.) and Boris W. Morukov (RSA). The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1500

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- At SPACEHAB, in Titusville, Fla., STS-101 crew members take part in a Crew Equipment Interface Test (CEIT). Here checking out the SPACEHAB Logistics Double Module are (left) Mission Specialists Mary Ellen Weber (Ph.D.), and (right) Edward Tsang Lu (Ph.D.). Other members of the crew taking part in the CEIT are Commander James Donald Halsell Jr., Pilot Scott J. "Doc" Horowitz (Ph.D.), and Mission Specialists Jeffrey N. Williams, and Yuri Malenchenko and Boris W. Morukov, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1498

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Titusville, Fla., STS-101 crew members check out the SPACEHAB Logistics Double Module that will be part of the payload for their mission. From left are Pilot Scott J. "Doc" Horowitz (Ph.D.), and Mission Specialists Edward Tsang Lu (Ph.D.) and Mary Ellen Weber (Ph.D.). Other crew members taking part in the CEIT are Commander James Donald Halsell Jr., Jeffrey N. Williams, and Yuri Malenchenko and Boris W. Morukov, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1491

NASA Image and Video Library

1999-12-09

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., STS-101 crew members Edward Tsang Lu (Ph.D.) and Yuri Malenchenko, who is with the Russian Space Agency (RSA) check out part of the Russian crane Strela. Other crew members are Commander James Donald Halsell Jr., Pilot Scott Horowitz, and Mission Specialists Jeffrey N. Williams, Mary Ellen Weber, (Ph.D.) and Boris W. Morukov, also with RSA. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1493

NASA Image and Video Library

1999-12-09

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., STS-101 crew members check out some of the cargo that will be carried on their mission. From left are Mission Specialists Boris W. Morukov, who is with the Russian Space Agency (RSA), Jeffrey N. Williams, and Yuri Malenchenko, also with RSA. Other crew members are Commander James Donald Halsell Jr., Pilot Scott J. "Doc" Horowitz (Ph.D.) and Mission Specialists Mary Ellen Weber, (Ph.D.) and Edward Tsang Lu (Ph.D.). The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1492

NASA Image and Video Library

1999-12-09

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., STS-101 crew members check out some of the cargo that will be carried on their mission. From left are Pilot Scott J. "Doc" Horowitz (Ph.D.) and Mission Specialists Mary Ellen Weber, (Ph.D.), Jeffrey N. Williams, and Boris W. Morukov, who is with the Russian Space Agency (RSA). Other crew members are Commander James Donald Halsell Jr., Edward Tsang Lu (Ph.D.) and Yuri Malenchenko, also with RSA. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., STS-101 crew members check out some of the cargo that will be carried on their mission. From left are Pilot Scott J. 'Doc' Horowitz (Ph.D.) and Mission Specialists Mary Ellen Weber, (Ph.D.), Jeffrey N. Williams, and Boris W. Morukov, who is with the Russian Space Agency (RSA). Other crew members are Commander James Donald Halsell Jr., Edward Tsang Lu (Ph.D.) and Yuri Malenchenko, also with RSA. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., STS-101 crew members Edward Tsang Lu (Ph.D.) and Yuri Malenchenko, who is with the Russian Space Agency (RSA) check out part of the Russian crane Strela. Other crew members are Commander James Donald Halsell Jr., Pilot Scott Horowitz, and Mission Specialists Jeffrey N. Williams, Mary Ellen Weber, (Ph.D.) and Boris W. Morukov, also with RSA. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., members of the STS-101 crew learn how to manipulate the Russian crane Strela. At left is Yuri Malenchenko, who is with the Russian Space Agency (RSA); in the center is Edward Tsang Lu (Ph.D.); at right is Mission Specialist Jeffrey N. Williams. Other crew members are Commander James Donald Halsell Jr., Pilot Scott Horowitz, and Mission Specialists Mary Ellen Weber, (Ph.D.) and Boris W. Morukov (RSA). The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT), members of the STS-101 crew learn about some of the cargo that will be on their mission from workers at SPACEHAB, in Cape Canaveral, Fla. At left are Commander James Donald Halsell Jr., and Mission Specialist Mary Ellen Weber, (Ph.D.). Other crew members are Pilot Scott Horowitz, and Mission Specialists Edward Lu, Jeffrey N. Williams, and Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT), members of the STS-101 crew learn about some of the cargo that will be on their mission from workers at SPACEHAB, in Cape Canaveral, Fla. At left are Mission Specialists Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. Other crew members are Commander James Donald Halsell Jr., Pilot Scott Horowitz, and Mission Specialists Mary Ellen Weber (Ph.D.), Edward Lu, and Jeffrey N. Williams, The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

CSG test

NASA Image and Video Library

2011-09-15

E-2 Test Stand team members at Stennis Space Center conducted their first series of tests on a three-module chemical steam generator unit Sept. 15. All three modules successfully fired during the tests. The chemical steam generator is a critical component for the A-3 Test Stand under construction at Stennis.

Interactive Videodisc Design and Production Workshop Guide.

ERIC Educational Resources Information Center

Campbell, J. Olin; And Others

This "how to" workshop guide provides an overview of the entire videodisc authoring and production process through six individual modules. Focusing on project planning, the first module provides guidelines, procedures, and job aids to help each instructional development team member effectively use the videodisc medium. The second module…

Greeks in America; Staff Development Module.

ERIC Educational Resources Information Center

Lereah, Lucy; And Others

This module lists eight staff development objectives pertaining to various aspects of Greek-American culture. Topics dealt with include Greek emigration and immigration, Greek vocabulary, contributions made by prominent Greek-Americans, Greek family life and the changing role of family members, Greek values, and the growth of Atlanta's Greek…

Does Sex of Dyad Members Really Matter? A Review of Leader-Member Exchange

ERIC Educational Resources Information Center

Goertzen, Brent J.; Fritz, Susan M.

2004-01-01

Leader-member exchange (LMX) generally refers to the leadership process centered on the interactions between leaders and direct reports. The basic premise of high quality leader-member exchange relationships holds that direct reports gain tremendous benefits through these partnerships. LMX is perhaps the most commonly researched theory of…

Stick-slip Cycles and Tidal Modulation of Ice Stream Flow

NASA Astrophysics Data System (ADS)

Lipovsky, B.; Dunham, E. M.

2016-12-01

The reactivation of a single dormant Antarctic ice stream would double the continent's mass imbalance. Despite importance of understanding the likelihood of such an event, direct observation of the basal processes that lead to the activation and stagnation of streaming ice are minimal. As the only ice stream undergoing stagnation, the Whillans Ice Plain (WIP) occupies a central role in our understanding of these subglacial processes. Complicating matters is the observation, from GPS records, that the WIP experiences most of its motion during episodes of rapid sliding. These sliding events are tidally modulated and separated by 12 hour periods of quiescence. We conduct numerical simulations of ice stream stick-slip cycles. Our simulations include rate- and state-dependent frictional sliding, tidal forcing, inertia, upstream loading in a cross-stream, thickness-averaged formulation. Our principal finding is that ice stream motion may respond to ocean tidal forcing with one of two end member behaviors. In one limit, tidally modulated slip events have rupture velocities that approach the shear wave speed and slip events have a duration that scales with the ice stream width divided by the shear wave speed. In the other limit, tidal modulation results in ice stream sliding velocities with lower amplitude variation but at much longer timescales, i.e. semi-diurnal and longer. This latter behavior more closely mimics the behavior of several active ice streams (Bindschadler, Rutford). We find that WIP slip events exist between these two end member behaviors: rupture velocities are far below the inertial limit yet sliding occurs only episodically. The continuum of sliding behaviors is governed by a critical ice stream width over which slip event nucleate. When the critical width is much longer than the ice stream width, slip events are unable to nucleate. The critical width depends on the subglacial effective pressure, ice thickness, and frictional and elastic constitutive parameters. One implication of our work is that, because the transition from steady to episodic sliding may occur by changing subglacial effective pressure, changing effective pressure may be responsible for the stagnation of the WIP.

KSC-2009-6512

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Bernardo Patti, at left, head of International Space Station, Program Department, European Space Agency, congratulates Michael Suffredini, program manager, International Space Station, NASA, upon transfer of the ownership of node 3 for the International Space Station from the European Space Agency, or ESA, to NASA. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

KSC-2009-6511

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Bernardo Patti, at left, head of International Space Station, Program Department, European Space Agency, and Michael Suffredini, program manager, International Space Station, NASA, sign documents transferring the ownership of node 3 for the International Space Station from the European Space Agency, or ESA, to NASA. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

KSC-2009-6515

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Bernardo Patti, head of International Space Station, Program Department, European Space Agency, or ESA, is photographed with invited guests of ESA in front of node 3 for the International Space Station following a ceremony transferring the ownership of the node from ESA to NASA. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

KSC-2009-6516

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Bernardo Patti, center, head of International Space Station, Program Department, European Space Agency, or ESA, admires the node 3 for the International Space Station, which his agency provided, following a ceremony transferring the ownership of the node from ESA to NASA. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

Morphine and galectin-1 modulate HIV-1 infection of human monocytes-derived macrophages

PubMed Central

Reynolds, Jessica L.; Law, Wing Cheung; Mahajan, Supriya D.; Aalinkeel, Ravikumar; Nair, Bindukumar; Sykes, Donald E.; Mammen, Manoj J.; Yong, Ken-Tye; Hui, Rui; Prasad, Paras N.; Schwartz, Stanley A.

2012-01-01

Morphine is a widely abused, addictive drug that modulates immune function. Macrophages are a primary reservoir of HIV-1; therefore, they not only play a role in the development of this disease but also impact the overall course of disease progression. Galectin-1 is a member of a family of β-galactoside-binding lectins that are soluble adhesion molecules and that mediate direct cell-pathogen interactions during HIV-1 viral adhesion. Since the drug abuse epidemic and the HIV-1 epidemic are closely interrelated we propose that increased expression of galectin-1 induced by morphine may modulate HIV-1 infection of human monocytes-derived macrophages (MDM). Here, we show that galectin-1 gene and protein expression are potentiated by incubation with morphine. Confirming previous studies, morphine alone or galectin-1 alone enhance HIV-1 infection of MDM. Concomitant incubation with exogenous galectin-1 and morphine potentiated HIV-1 infection of MDM. We utilized a nanotechnology approach that uses gold nanorod-galectin-1 siRNA complexes (nanoplexes) to inhibit gene expression for galectin-1. We found that nanoplexes silenced gene expression for galectin-1 and the nanoplexes reversed the effects of morphine on galectin-1 expression. Furthermore, the effects of morphine on HIV-1 infection were reduced in the presence of the nanoplex. PMID:22430735

Commanding Constellations (Pipeline Architecture)

NASA Technical Reports Server (NTRS)

Ray, Tim; Condron, Jeff

2003-01-01

Providing ground command software for constellations of spacecraft is a challenging problem. Reliable command delivery requires a feedback loop; for a constellation there will likely be an independent feedback loop for each constellation member. Each command must be sent via the proper Ground Station, which may change from one contact to the next (and may be different for different members). Dynamic configuration of the ground command software is usually required (e.g. directives to configure each member's feedback loop and assign the appropriate Ground Station). For testing purposes, there must be a way to insert command data at any level in the protocol stack. The Pipeline architecture described in this paper can support all these capabilities with a sequence of software modules (the pipeline), and a single self-identifying message format (for all types of command data and configuration directives). The Pipeline architecture is quite simple, yet it can solve some complex problems. The resulting solutions are conceptually simple, and therefore, reliable. They are also modular, and therefore, easy to distribute and extend. We first used the Pipeline architecture to design a CCSDS (Consultative Committee for Space Data Systems) Ground Telecommand system (to command one spacecraft at a time with a fixed Ground Station interface). This pipeline was later extended to include gateways to any of several Ground Stations. The resulting pipeline was then extended to handle a small constellation of spacecraft. The use of the Pipeline architecture allowed us to easily handle the increasing complexity. This paper will describe the Pipeline architecture, show how it was used to solve each of the above commanding situations, and how it can easily be extended to handle larger constellations.

Crew Training- Apollo 9

NASA Image and Video Library

1969-02-24

S69-19858 (December 1968) --- Two members of the Apollo 9 prime crew participate in simulation training in the Apollo Lunar Module Mission Simulator (LMMS) at the Kennedy Space Center (KSC). On the left is astronaut James A. McDivitt, commander; and on the right is astronaut Russell L. Schweickart, lunar module pilot.

Internationalizing the Curriculum: One Instructor's Experience.

ERIC Educational Resources Information Center

McNamara, Lois L.

Using a Title VI Grant from the U.S. Department of Education, one faculty member at Valencia Community College (VCC) developed international or intercultural modules for existing courses to explore both Western and non-Western contributions to the humanities. During the first year of grant implementation, a module on Jewish and Islamic…

Health Instruction Packages: Consumer--Safety and Emergencies.

ERIC Educational Resources Information Center

Lown, Maris A.; And Others

Texts, illustrations, and exercises are provided in these six learning modules which were designed to teach the general public how to deal with several emergency situations. The first module, "Cardio-Pulmonary Resuscitation (CPR)," was designed by Maris A. Lown to teach members of the public, nurses, and paramedics the techniques of…

KSC-07pd0900

NASA Image and Video Library

2007-04-17

KENNEDY SPACE CENTER, FLA. -- After a welcoming ceremony for the Experiment Logistics Module Pressurized Section of the Japanese Experiment Module, astronaut Takao Doi (right) talks with Kumiko Tanabe, a public affairs representative of the Japanese Aerospace and Exploration Agency. The logistics module will be delivered to the space station on mission STS-123. Doi is a crew member on that mission.The module will serve as an on-orbit storage area for materials, tools and supplies. It can hold up to eight experiment racks and will attach to the top of another larger pressurized module. Photo credit: NASA/George Shelton

Behavioral modeling and digital compensation of nonlinearity in DFB lasers for multi-band directly modulated radio-over-fiber systems

NASA Astrophysics Data System (ADS)

Li, Jianqiang; Yin, Chunjing; Chen, Hao; Yin, Feifei; Dai, Yitang; Xu, Kun

2014-11-01

The envisioned C-RAN concept in wireless communication sector replies on distributed antenna systems (DAS) which consist of a central unit (CU), multiple remote antenna units (RAUs) and the fronthaul links between them. As the legacy and emerging wireless communication standards will coexist for a long time, the fronthaul links are preferred to carry multi-band multi-standard wireless signals. Directly-modulated radio-over-fiber (ROF) links can serve as a lowcost option to make fronthaul connections conveying multi-band wireless signals. However, directly-modulated radioover- fiber (ROF) systems often suffer from inherent nonlinearities from directly-modulated lasers. Unlike ROF systems working at the single-band mode, the modulation nonlinearities in multi-band ROF systems can result in both in-band and cross-band nonlinear distortions. In order to address this issue, we have recently investigated the multi-band nonlinear behavior of directly-modulated DFB lasers based on multi-dimensional memory polynomial model. Based on this model, an efficient multi-dimensional baseband digital predistortion technique was developed and experimentally demonstrated for linearization of multi-band directly-modulated ROF systems.

Systems and methods for reconfiguring input devices

NASA Technical Reports Server (NTRS)

Lancaster, Jeff (Inventor); De Mers, Robert E. (Inventor)

2012-01-01

A system includes an input device having first and second input members configured to be activated by a user. The input device is configured to generate activation signals associated with activation of the first and second input members, and each of the first and second input members are associated with an input function. A processor is coupled to the input device and configured to receive the activation signals. A memory coupled to the processor, and includes a reconfiguration module configured to store the input functions assigned to the first and second input members and, upon execution of the processor, to reconfigure the input functions assigned to the input members when the first input member is inoperable.

Modulation of eukaryotic cell apoptosis by members of the bacterial order Actinomycetales.

PubMed

Barry, Daniel P; Beaman, Blaine L

2006-10-01

Apoptosis, or programmed cell death, is normally responsible for the orderly elimination of aged or damaged cells, and is a necessary part of the homeostasis and development of multicellular organisms. Some pathogenic bacteria can disrupt this process by triggering excess apoptosis or by preventing it when appropriate. Either event can lead to disease. There has been extensive research into the modulation of host cell death by microorganisms, and several reviews have been published on the phenomenon. Rather than covering the entire field, this review focuses on the dysregulation of host cell apoptosis by members of the order Actinomycetales, containing the genera Corynebacterium, Mycobacterium, Rhodococcus, and Nocardia.

KSC-07pd2827

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, members of the STS-123 crew get hands-on experience with the mission payload, the Kibo Experiment Logistics Module Pressurized Section. They are at the center for a crew equipment interface test, which allows familiarization with equipment they will use during the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

KSC-07pd2828

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, members of the STS-123 crew get hands-on experience with the mission payload, the Kibo Experiment Logistics Module Pressurized Section. They are at the center for a crew equipment interface test, which allows familiarization with equipment they will use during the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

KSC-07pd2826

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, members of the STS-123 crew learn more about the mission payload, the Kibo Experiment Logistics Module Pressurized Section. They are at the center for a crew equipment interface test, which allows familiarization with equipment they will use during the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

Emerging roles and regulation of MiT/TFE transcriptional factors.

PubMed

Yang, Min; Liu, En; Tang, Li; Lei, Yuanyuan; Sun, Xuemei; Hu, Jiaxi; Dong, Hui; Yang, Shi-Ming; Gao, Mingfa; Tang, Bo

2018-06-15

The MiT/TFE transcription factors play a pivotal role in the regulation of autophagy and lysosomal biogenesis. The subcellular localization and activity of MiT/TFE proteins are primarily regulated through phosphorylation. And the phosphorylated protein is retained in the cytoplasm and subsequently translocates to the nucleus upon dephosphorylation, where it stimulates the expression of hundreds of genes, leading to lysosomal biogenesis and autophagy induction. The transcription factor-mediated lysosome-to-nucleus signaling can be directly controlled by several signaling molecules involved in the mTORC1, PKC, and AKT pathways. MiT/TFE family members have attracted much attention owing to their intracellular clearance of pathogenic factors in numerous diseases. Recently, multiple studies have also revealed the MiT/TFE proteins as master regulators of cellular metabolic reprogramming, converging on autophagic and lysosomal function and playing a critical role in cancer, suggesting that novel therapeutic strategies could be based on the modulation of MiT/TFE family member activity. Here, we present an overview of the latest research on MiT/TFE transcriptional factors and their potential mechanisms in cancer.

Modulation of beta-amyloid precursor protein trafficking and processing by the low density lipoprotein receptor family.

PubMed

Cam, Judy A; Bu, Guojun

2006-08-18

Amyloid-beta peptide (Abeta) accumulation in the brain is an early, toxic event in the pathogenesis of Alzheimer's disease (AD). Abeta is produced by proteolytic processing of a transmembrane protein, beta-amyloid precursor protein (APP), by beta- and gamma-secretases. Mounting evidence has demonstrated that alterations in APP cellular trafficking and localization directly impact its processing to Abeta. Recent studies have shown that members of the low-density lipoprotein receptor family, including LRP, LRP1B, SorLA/LR11, and apolipoprotein E (apoE) receptor 2, interact with APP and regulate its endocytic trafficking. Another common feature of these receptors is their ability to bind apoE, which exists in three isoforms in humans and the presence of the epsilon4 allele represents a genetic risk factor for AD. In this review, we summarize the current understanding of the function of these apoE receptors with a focus on their role in APP trafficking and processing. Knowledge of the interactions between these distinct low-density lipoprotein receptor family members and APP may ultimately influence future therapies for AD.

Targeting and transport: How microtubules control focal adhesion dynamics

PubMed Central

Stehbens, Samantha

2012-01-01

Directional cell migration requires force generation that relies on the coordinated remodeling of interactions with the extracellular matrix (ECM), which is mediated by integrin-based focal adhesions (FAs). Normal FA turnover requires dynamic microtubules, and three members of the diverse group of microtubule plus-end-tracking proteins are principally involved in mediating microtubule interactions with FAs. Microtubules also alter the assembly state of FAs by modulating Rho GTPase signaling, and recent evidence suggests that microtubule-mediated clathrin-dependent and -independent endocytosis regulates FA dynamics. In addition, FA-associated microtubules may provide a polarized microtubule track for localized secretion of matrix metalloproteases (MMPs). Thus, different aspects of the molecular mechanisms by which microtubules control FA turnover in migrating cells are beginning to emerge. PMID:22908306

Apparatus and method for interaction phenomena with world modules in data-flow-based simulation

DOEpatents

Xavier, Patrick G [Albuquerque, NM; Gottlieb, Eric J [Corrales, NM; McDonald, Michael J [Albuquerque, NM; Oppel, III, Fred J.

2006-08-01

A method and apparatus accommodate interaction phenomenon in a data-flow-based simulation of a system of elements, by establishing meta-modules to simulate system elements and by establishing world modules associated with interaction phenomena. World modules are associated with proxy modules from a group of meta-modules associated with one of the interaction phenomenon. The world modules include a communication world, a sensor world, a mobility world, and a contact world. World modules can be further associated with other world modules if necessary. Interaction phenomenon are simulated in corresponding world modules by accessing member functions in the associated group of proxy modules. Proxy modules can be dynamically allocated at a desired point in the simulation to accommodate the addition of elements in the system of elements such as a system of robots, a system of communication terminals, or a system of vehicles, being simulated.

The HTLV-I tax protein transcriptionally modulates OX40 antigen expression.

PubMed

Pankow, R; Dürkop, H; Latza, U; Krause, H; Kunzendorf, U; Pohl, T; Bulfone-Paus, S

2000-07-01

OX40 is a member of the TNF receptor family, expressed on activated T cells. It is the only costimulatory T cell molecule known to be specifically up-regulated in human T cell leukemia virus type-I (HTLV-I)-producing cells. In a T cell line, OX40 surface expression was shown to be induced by HTLV-I Tax alone. To understand molecular mechanisms of OX40 gene regulation and modulation by HTLV-I Tax, we have cloned the human OX40 gene and analyzed its 5'-flanking region. By reporter gene analysis with progressive 5' deletions from nucleotides -1259 to -64, we have defined a 157-bp DNA fragment as a minimal promoter for constitutive expression. In addition, we show that in the OX40+ cell line, Co, Tax is able to further increase OX40 surface expression. Up-regulation of OX40 promoter activity by Tax requires two upstream NF-kappaB sites, which are not active in the constitutive OX40 expression. Their deletion abrogates Tax responsiveness in reporter gene analysis. The site-directed mutagenesis of each NF-kappaB site demonstrates that cooperative NF-kappaB binding is a prerequisite for Tax-directed activity as neither site alone is sufficient for a full Tax responsiveness of the OX40 promoter. Upon Tax expression, both sites bind p65 and c-Rel. These data provide new insight into the direct regulation of OX40 by Tax and add to our understanding of the possible role of the OX40/OX40 ligand system in the proliferation of HTLV-I+ T cells.

Reconstruction of the genome-scale co-expression network for the Hippo signaling pathway in colorectal cancer.

PubMed

Dehghanian, Fariba; Hojati, Zohreh; Hosseinkhan, Nazanin; Mousavian, Zaynab; Masoudi-Nejad, Ali

2018-05-26

The Hippo signaling pathway (HSP) has been identified as an essential and complex signaling pathway for tumor suppression that coordinates proliferation, differentiation, cell death, cell growth and stemness. In the present study, we conducted a genome-scale co-expression analysis to reconstruct the HSP in colorectal cancer (CRC). Five key modules were detected through network clustering, and a detailed discussion of two modules containing respectively 18 and 13 over and down-regulated members of HSP was provided. Our results suggest new potential regulatory factors in the HSP. The detected modules also suggest novel genes contributing to CRC. Moreover, differential expression analysis confirmed the differential expression pattern of HSP members and new suggested regulatory factors between tumor and normal samples. These findings can further reveal the importance of HSP in CRC. Copyright © 2018 Elsevier Ltd. All rights reserved.

KSC-2010-1134

NASA Image and Video Library

2010-01-08

CAPE CANAVERAL, Fla. - In Orbiter Processing Facility 3 at NASA's Kennedy Space Center in Florida, members of space shuttle Discovery's STS-131 crew participate in training activities during the Crew Equipment Interface Test, or CEIT, for their mission. Here, Pilot James P. Dutton Jr. experiences the feel of the cockpit from inside the crew module. The CEIT provides the crew with hands-on training and observation of shuttle and flight hardware. The seven-member crew will deliver the multi-purpose logistics module Leonardo, filled with resupply stowage platforms and racks to be transferred to locations around the International Space Station. Three spacewalks will include work to attach a spare ammonia tank assembly to the station's exterior and return a European experiment from outside the station's Columbus module. Discovery's launch is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Kim Shiflett

High flow rate nozzle system with production of uniform size droplets

DOEpatents

Stockel, I.H.

1990-10-16

Method steps for production of substantially uniform size droplets from a flow of liquid include forming the flow of liquid, periodically modulating the momentum of the flow of liquid in the flow direction at controlled frequency, generating a cross flow direction component of momentum and modulation of the cross flow momentum of liquid at substantially the same frequency and phase as the modulation of flow direction momentum, and spraying the so formed modulated flow through a first nozzle outlet to form a desired spray configuration. A second modulated flow through a second nozzle outlet is formed according to the same steps, and the first and second modulated flows impinge upon each other generating a liquid sheet. Nozzle apparatus for modulating each flow includes rotating valving plates interposed in the annular flow of liquid. The plates are formed with radial slots. Rotation of the rotating plates is separably controlled at differential angular velocities for a selected modulating frequency to achieve the target droplet size and production rate for a given flow. The counter rotating plates are spaced to achieve a desired amplitude of modulation in the flow direction, and the angular velocity of the downstream rotating plate is controlled to achieve the desired amplitude of modulation of momentum in the cross flow direction. Amplitude of modulation is set according to liquid viscosity. 5 figs.

High flow rate nozzle system with production of uniform size droplets

DOEpatents

Stockel, Ivar H.

1990-01-01

Method steps for production of substantially uniform size droplets from a flow of liquid include forming the flow of liquid, periodically modulating the momentum of the flow of liquid in the flow direction at controlled frequency, generating a cross flow direction component of momentum and modulation of the cross flow momentum of liquid at substantially the same frequency and phase as the modulation of flow direction momentum, and spraying the so formed modulated flow through a first nozzle outlet to form a desired spray configuration. A second modulated flow through a second nozzle outlet is formed according to the same steps, and the first and second modulated flows impinge upon each other generating a liquid sheet. Nozzle apparatus for modulating each flow includes rotating valving plates interposed in the annular flow of liquid. The plates are formed with radial slots. Rotation of the rotating plates is separably controlled at differential angular velocities for a selected modulating frequency to achieve the target droplet size and production rate for a given flow. The counter rotating plates are spaced to achieve a desired amplitude of modulation in the flow direction, and the angular velocity of the downstream rotating plate is controlled to achieve the desired amplitude of modulation of momentum in the cross flow direction. Amplitude of modulation is set according to liquid viscosity.

Mangroves and Seawalls. "Increased Pressure for Land Fill Will Cause More and More Stress to Natural Areas." Grades 7 and 8. A Three Lesson Unit. Student Learning Activity Module.

ERIC Educational Resources Information Center

Frank, James

This module is an activity/discussion-centered unit focusing on the importance of shoreline surface area. The module is part of a series designed to be used by teachers, students, and community members to help them utilize community resources in developing and teaching environmental concepts and responsibility, and in seeking ways to solve…

Lipopeptides as main ingredients for inhibition of fungal phytopathogens by Bacillus subtilis/amyloliquefaciens

PubMed Central

Cawoy, Hélène; Debois, Delphine; Franzil, Laurent; De Pauw, Edwin; Thonart, Philippe; Ongena, Marc

2015-01-01

Some isolates of the Bacillus subtilis/amyloliquefaciens species are known for their plant protective activity against fungal phytopathogens. It is notably due to their genetic potential to form an impressive array of antibiotics including non-ribosomal lipopeptides (LPs). In the work presented here, we wanted to gain further insights into the relative role of these LPs in the global antifungal activity of B. subtilis/amyloliquefaciens. To that end, a comparative study was conducted involving multiple strains that were tested against four different phytopathogens. We combined various approaches to further exemplify that secretion of those LPs is a crucial trait in direct pathogen ward off and this can actually be generalized to all members of these species. Our data illustrate that for each LP family, the fungitoxic activity varies in function of the target species and that the production of iturins and fengycins is modulated by the presence of pathogens. Our data on the relative involvement of these LPs in the biocontrol activity and modulation of their production are discussed in the context of natural conditions in the rhizosphere. PMID:25529983

Vegetarian diets and gut microbiota: important shifts in markers of metabolism and cardiovascular disease.

PubMed

do Rosario, Vinicius A; Fernandes, Ricardo; Trindade, Erasmo B S de M

2016-07-01

Vegetarian diets have been associated with a lower incidence of several chronic diseases. The benefits of plant-based diets are related mainly to the improvement of metabolic parameters that can indicate risk for such diseases. Some metabolic factors, such as oxidative balance, lipid profile, and glucose homeostasis, can be improved directly by diet, but paradoxically, some characteristics of vegetarian diets may promote a negative scenario that increases the risk of certain chronic diseases. Additionally, many benefits of a vegetarian diet are mediated by the gut microbiota, members of which not only have taxonomic and functional differences but also produce diverse, specific metabolites that vary according to whether the host consumes an omnivorous or a vegetarian diet. This review examines the modulation of human metabolism and gut microbiota by vegetarian and omnivorous dietary patterns and explores how this modulation may affect the risk of cardiovascular disease. © The Author(s) 2016. Published by Oxford University Press on behalf of the International Life Sciences Institute. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

PIP₂ modulation of Slick and Slack K⁺ channels.

PubMed

de los Angeles Tejada, Maria; Jensen, Lars Jørn; Klaerke, Dan A

2012-07-27

Slick and Slack are members of the Slo family of high-conductance potassium channels. These channels are activated by Na(+) and Cl(-) and are highly expressed in the CNS, where they are believed to contribute to the resting membrane potential of neurons and the control of excitability. Herein, we provide evidence that Slick and Slack channels are regulated by the phosphoinositide PIP(2). Two stereoisomers of PIP(2) were able to exogenously activate Slick and Slack channels expressed in Xenopus oocytes, and in addition, it is shown that Slick and Slack channels are modulated by endogenous PIP(2). The activating effect of PIP(2) appears to occur by direct interaction with lysine 306 in Slick and lysine 339 in Slack, located at the proximal C-termini of both channels. Overall, our data suggest that PIP(2) is an important regulator of Slick and Slack channels, yet it is not involved in the recently described cell volume sensitivity of Slick channels, since mutated PIP(2)-insensitive Slick channels retained their sensitivity to cell volume. Copyright © 2012 Elsevier Inc. All rights reserved.

The Joint Airlock Module is moved to a payload canister in the O&C

NASA Technical Reports Server (NTRS)

2000-01-01

The Joint Airlock Module is suspended by an overhead crane in the Operations and Checkout Building before being moved and placed into the payload canister for transfer to the Space Station Processing Facility. There the module will undergo more preflight processing for the STS-104 mission scheduled for launch aboard Space Shuttle Atlantis May 17, 2001. The Joint Airlock Module is the gateway from which crew members aboard the International Space Station will enter and exit the 470-ton orbiting research facility.

Direct-Sequence Spread Spectrum System

DTIC Science & Technology

1990-06-01

by directly modulating a conventional narrowband frequency-modulated (FM) carrier by a high rate digital code. The direct modulation is binary phase ...specification of the DSSS system will not be developed. The results of the evaluation phase of this research will be compared against theoretical...spread spectrum is called binary phase -shift keying 19 (BPSK). BPSK is a modulation in which a binary Ŕ" represents a 0-degree relative phase

26 CFR 56.4911-5 - Communications with members.

Code of Federal Regulations, 2011 CFR

2011-04-01

... lobbying expenditures even though those expenditures would be grass roots expenditures if the communication... communication does not directly encourage the member to engage in grass roots lobbying (whether individually or... lobbying. (d) Communications (directed only to members) that are grass roots lobbying communications...

26 CFR 56.4911-5 - Communications with members.

Code of Federal Regulations, 2010 CFR

2010-04-01

... lobbying expenditures even though those expenditures would be grass roots expenditures if the communication... communication does not directly encourage the member to engage in grass roots lobbying (whether individually or... lobbying. (d) Communications (directed only to members) that are grass roots lobbying communications...

Direct access and patient/client self-referral to physiotherapy: a review of contemporary practice within the European Union.

PubMed

Bury, T J; Stokes, E K

2013-12-01

Direct access refers to service users being able to refer themselves to physiotherapy without a third-party referral. It represents a model of practice supported globally by the profession, growing research evidence and health policy in some health systems. To the authors' knowledge, no research has been reported to ascertain the extent to which direct access is available within the physiotherapy profession within the European Union (EU). To survey member organisations of the World Confederation for Physical Therapy (WCPT); establish the number of member states within the EU where it is possible for individuals seeking physiotherapy services to self-refer; describe the legislative/regulatory and reimbursement contexts in which physiotherapy services are delivered; examine if physiotherapy practice is different in member states where direct access is permitted compared with member states where direct access is not permitted; and to describe the barriers and facilitators to direct access perceived by member organisations of the WCPT. Cross-sectional, online survey using a purposive sample. Member organisations of the WCPT in the EU. Direct access is not available in all member states of the EU, despite the majority having legislation to regulate the profession, and entry-level education programmes that produce graduates with the requisite competencies. Key barriers perceived are those that can influence policy development, including the views of the medical profession and politicians. Support of service users and politicians, as well as professional autonomy, are seen as key facilitators. These results represent the first report of a comprehensive mapping of direct access to physiotherapy and contexts within the EU. In over half of member states, service users can self-refer to physiotherapists. These results provide insights to further individuals' understanding about the similarities and differences in working practices and service delivery factors, such as reimbursement across and within EU member states. The synergies between barriers and facilitators indicate the importance of targeted advocacy strategies in the introduction of direct access/self-referral to physiotherapy. Copyright © 2013 Chartered Society of Physiotherapy. Published by Elsevier Ltd. All rights reserved.

Basic Fishing. Aquatic Skills Series. Instructor Manual.

ERIC Educational Resources Information Center

Staton, Robert D., Jr.

Part of a series of self-contained instructional units to teach Missourians how to use outdoor resources wisely and skillfully, this module of the Aquatic Skills Series introduces the basics of sport fishing using spin-casting equipment. The instructor may modify the module to meet the individual needs of class members based on age, experience…

Health Instruction Packages: Consumer--Understanding Your Condition.

ERIC Educational Resources Information Center

Kidd, Audrey E.; And Others

Text, illustrations, and exercises are utilized in this set of three learning modules to inform members of the general public about the causes and treatments of the common medical ailments that they may have. The first module, "Understanding Ligation of Hemorrhoids as an Office Procedure" by Audrey E. Kidd, lists the symptoms of…

PORTRAIT - APOLLO 7

NASA Image and Video Library

1968-02-13

S68-21590 (September 1968) --- This is a portrait of the Apollo-Saturn 7 crew members. They are, left to right, astronauts Walter M. Schirra Jr., commander; Walter Cunningham, lunar module pilot; and Donn F. Eisele, command module pilot. EDITOR'S NOTE: Since this photograph was made astronaut Eisele died Dec. 2, 1987 in Tokyo, Japan, of a heart attack.

MS Kavandi with camera in Service Module

NASA Image and Video Library

2001-07-16

STS104-E-5125 (16 July 2001) --- Astronaut Janet L. Kavandi, STS-104 mission specialist, uses a camera as she floats through the Zvezda service module aboard the International Space Station (ISS). The five STS-104 crew members were visiting the orbital outpost to perform various tasks. The image was recorded with a digital still camera.

Solar module having reflector between cells

DOEpatents

Kardauskas, Michael J.

1999-01-01

A photovoltaic module comprising an array of electrically interconnected photovoltaic cells disposed in a planar and mutually spaced relationship between a light-transparent front cover member in sheet form and a back sheet structure is provided with a novel light-reflecting means disposed between adjacent cells for reflecting light falling in the areas between cells back toward said transparent cover member for further internal reflection onto the solar cells. The light-reflecting comprises a flexible plastic film that has been embossed so as to have a plurality of small V-shaped grooves in its front surface, and a thin light-reflecting coating on said front surface, the portions of said coating along the sides of said grooves forming light-reflecting facets, said grooves being formed so that said facets will reflect light impinging thereon back into said transparent cover sheet with an angle of incidence greater than the critical angle, whereby substantially all of the reflected light will be internally reflected from said cover sheet back to said solar modules, thereby increasing the current output of the module.

KSC-07pd2840

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, members of the STS-123 crew get hands-on experience with some of the equipment related to the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

KSC-07pd2842

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, members of the STS-123 crew get hands-on experience with some of the equipment related to the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

KSC-07pd2844

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, members of the STS-123 crew get hands-on experience with some of the equipment related to the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

KSC-07pd2845

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, members of the STS-123 crew get hands-on experience with some of the equipment related to the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

KSC-07pd2841

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, members of the STS-123 crew get hands-on experience with some of the equipment related to the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

KSC-99pp1489

NASA Image and Video Library

1999-12-09

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., members of the STS-101 crew learn about some of the cargo that will be on their mission. At left are Mission Specialists Jeffrey N. Williams and Edward Tsang Lu (Ph.D.); at right are Commander James Donald Halsell Jr., and Mission Specialist Boris W. Morukov, who is with the Russian Space Agency (RSA). Other crew members are Pilot Scott Horowitz, and Mission Specialists Mary Ellen Weber, (Ph.D.) and Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1494

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Titusville, Fla., STS-101 crew members check out the SPACEHAB Logistics Double Module that will be part of the payload for their mission. At left are Commander James Donald Halsell Jr. and Pilot Scott J. "Doc" Horowitz (Ph.D.); seated on the floor is Mission Specialist Edward Tsang Lu (Ph.D.). Other crew members who are taking part in the CEIT are Mission Specialists Mary Ellen Weber, (Ph.D.), Jeffrey N. Williams, and Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1497

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Titusville, Fla., STS-101 crew members check out the SPACEHAB Logistics Double Module that will be part of the payload for their mission. At right is Mission Specialist Mary Ellen Weber (Ph.D.), who is assisted by a SPACEHAB worker. Other crew members taking part in the CEIT are Commander James Donald Halsell Jr., Pilot Scott J. "Doc" Horowitz (Ph.D.), and Mission Specialists Edward Tsang Lu (Ph.D.), Jeffrey N. Williams, and Yuri Malenchenko and Boris W. Morukov, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1495

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Titusville, Fla., STS-101 crew members check out the SPACEHAB Logistics Double Module that will be part of the payload for their mission. From left are Commander James Donald Halsell Jr., Mission Specialist Mary Ellen Weber, (Ph.D.), Pilot Scott J. "Doc" Horowitz (Ph.D.), and Mission Specialist Edward Tsang Lu (Ph.D.). Other crew members who are taking part in the CEIT are Mission Specialists Jeffrey N. Williams, and Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., STS-101 crew members check out some of the cargo that will be carried on their mission. From left are Mission Specialists Boris W. Morukov, who is with the Russian Space Agency (RSA), Jeffrey N. Williams, and Yuri Malenchenko, also with RSA. Other crew members are Commander James Donald Halsell Jr., Pilot Scott J. 'Doc' Horowitz (Ph.D.) and Mission Specialists Mary Ellen Weber, (Ph.D.) and Edward Tsang Lu (Ph.D.). The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Titusville, Fla., STS-101 crew members check out the SPACEHAB Logistics Double Module that will be part of the payload for their mission. At right is Mission Specialist Mary Ellen Weber (Ph.D.), who is assisted by a SPACEHAB worker. Other crew members taking part in the CEIT are Commander James Donald Halsell Jr., Pilot Scott J. 'Doc' Horowitz (Ph.D.), and Mission Specialists Edward Tsang Lu (Ph.D.), Jeffrey N. Williams, and Yuri Malenchenko and Boris W. Morukov, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Titusville, Fla., STS-101 crew members check out the SPACEHAB Logistics Double Module that will be part of the payload for their mission. From left are Commander James Donald Halsell Jr., Mission Specialist Mary Ellen Weber, (Ph.D.), Pilot Scott J. 'Doc' Horowitz (Ph.D.), and Mission Specialist Edward Tsang Lu (Ph.D.). Other crew members who are taking part in the CEIT are Mission Specialists Jeffrey N. Williams, and Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Titusville, Fla., STS-101 crew members check out the SPACEHAB Logistics Double Module that will be part of the payload for their mission. At left are Commander James Donald Halsell Jr. and Pilot Scott J. 'Doc' Horowitz (Ph.D.); seated on the floor is Mission Specialist Edward Tsang Lu (Ph.D.). Other crew members who are taking part in the CEIT are Mission Specialists Mary Ellen Weber, (Ph.D.), Jeffrey N. Williams, and Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Cape Canaveral, Fla., members of the STS-101 crew learn about some of the cargo that will be on their mission. At left are Mission Specialists Jeffrey N. Williams and Edward Tsang Lu (Ph.D.); at right are Commander James Donald Halsell Jr., and Mission Specialist Boris W. Morukov, who is with the Russian Space Agency (RSA). Other crew members are Pilot Scott Horowitz, and Mission Specialists Mary Ellen Weber, (Ph.D.) and Boris W. Morukov and Yuri Malenchenko, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB's Logistics Double Module. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

[Change of host's behavior including man under the influence of parasites].

PubMed

Sergiev, V P

2010-01-01

Directed modulation of hosts' behavior favouring transmission of pathogen was noted in many parasites and, above all, in helminthes, which life cycle includes the consequent change of several hosts. It turned out that parasites use the same neuromediators for change of behavior of both mammals and hosts belonging to other animal classes. In fishes as well as in mammals, monoamines-neurotransmitters assist in brain functioning. Norepinephrine, dopamine and serotonin affect the alimentation, motion activity, aggression and social behaviour. Changes in concentration ratio of serotonin and its metabolites in invaded species were more pronounced, which pointed to directed effects of pathogens on serotonin activity. The same effect of some pathogens on human behaviour does not have selective significance because humans are not an essential link in life cycle of many parasites. Although the mentioned effect on behaviour could lead to negative consequences. For examples, persons with latent toxoplasmosis are significantly more frequent become members or victims of traffic accidents due to decreased ability for concentration of attention.

An AWG-based 10 Gbit/s colorless WDM-PON system using a chirp-managed directly modulated laser

NASA Astrophysics Data System (ADS)

Latif, Abdul; Yu, Chong-xiu; Xin, Xiang-jun; Husain, Aftab; Hussain, Ashiq; Munir, Abid; Khan, Yousaf

2012-09-01

We propose an arrayed waveguide grating (AWG)-based 10 Gbit/s per channel full duplex wavelength division multiplexing passive optical network (WDM-PON). A chirp managed directly modulated laser with return-to-zero (RZ) differential phase shift keying (DPSK) modulation technique is utilized for downlink (DL) direction, and then the downlink signal is re-modulated for the uplink (UL) direction using intensity modulation technique with the data rate of 10 Gbit/s per channel. A successful WDM-PON transmission operation with the data rate of 10 Gbit/s per channel over a distance of 25 km without any optical amplification or dispersion compensation is demonstrated with low power penalty.

ASTP crewmen in Apollo Command Module Trainer during training session at JSC

NASA Technical Reports Server (NTRS)

1975-01-01

The three members of the American ASTP prime crew are photographed inside the Apollo Command Module (CM) trainer in a water tank in bldg 260 during water egress training at JSC. They are, left to right, Astronauts Thomas P. Stafford, commander; Vance D. Brand, command module pilot; and Donald K. Slayton, docking module pilot (23430); Slayton attaches his life preserver as he egresses an Apollo Command Module trainer in a water tank in bldg 260 during water egresss training at JSC. Astronauts Brand (on left) and Stafford have already egressed the trainer and are seated in a three-man life raft.

Methods and apparatus for controlling rotary machines

DOEpatents

Bagepalli, Bharat Sampathkumaran [Niskayuna, NY; Jansen, Patrick Lee [Scotia, NY; Barnes, Gary R [Delanson, NY; Fric, Thomas Frank [Greer, SC; Lyons, James Patrick Francis [Niskayuna, NY; Pierce, Kirk Gee [Simpsonville, SC; Holley, William Edwin [Greer, SC; Barbu, Corneliu [Guilderland, NY

2009-09-01

A control system for a rotary machine is provided. The rotary machine has at least one rotating member and at least one substantially stationary member positioned such that a clearance gap is defined between a portion of the rotating member and a portion of the substantially stationary member. The control system includes at least one clearance gap dimension measurement apparatus and at least one clearance gap adjustment assembly. The adjustment assembly is coupled in electronic data communication with the measurement apparatus. The control system is configured to process a clearance gap dimension signal and modulate the clearance gap dimension.

Structural Assembly for Cold Plate Cooling

NASA Technical Reports Server (NTRS)

Zaffetti, Mark A. (Inventor); Taddey, Edmund P. (Inventor)

2014-01-01

A device including a structural member having a heat spreader and an electronic device mounted directly to a first surface of the heat spreader of the structural member. The device also includes a cold plate mounted directly to the first surface of the heat spreader of the structural member.

KSC-00pp1605

NASA Image and Video Library

2000-10-23

Inside the U.S. Lab, Destiny, members of the STS-98 crew work with technicians (in the background) to learn more about the equipment in the module. They are taking part in Crew Equipment Interface Test activities. At left, back to camera, is Mission Specialist Marsha Ivins. Standing are Mission Specialists Thomas Jones (left) and Robert Curbeam (right). Other crew members not seen are Commander Ken Cockrell and Pilot Mark Polansky. The mission will be transporting the Lab to the International Space Station with five system racks already installed inside of the module. With delivery of electronics in the lab, electrically powered attitude control for Control Moment Gyroscopes will be activated. The STS-98 launch is scheduled for Jan. 18, 2001

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Soichi Noguchi arrives at KSC aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Soichi Noguchi arrives at KSC aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Stephen Robinson arrives at KSC aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Stephen Robinson arrives at KSC aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Charles Camarda arrives at KSC aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment,to the Space Station, and the external stowage platform.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Charles Camarda arrives at KSC aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment,to the Space Station, and the external stowage platform.

Polarization-interleave-multiplexed discrete multi-tone modulation with direct detection utilizing MIMO equalization.

PubMed

Zhou, Xian; Zhong, Kangping; Gao, Yuliang; Sui, Qi; Dong, Zhenghua; Yuan, Jinhui; Wang, Liang; Long, Keping; Lau, Alan Pak Tao; Lu, Chao

2015-04-06

Discrete multi-tone (DMT) modulation is an attractive modulation format for short-reach applications to achieve the best use of available channel bandwidth and signal noise ratio (SNR). In order to realize polarization-multiplexed DMT modulation with direct detection, we derive an analytical transmission model for dual polarizations with intensity modulation and direct diction (IM-DD) in this paper. Based on the model, we propose a novel polarization-interleave-multiplexed DMT modulation with direct diction (PIM-DMT-DD) transmission system, where the polarization de-multiplexing can be achieved by using a simple multiple-input-multiple-output (MIMO) equalizer and the transmission performance is optimized over two distinct received polarization states to eliminate the singularity issue of MIMO demultiplexing algorithms. The feasibility and effectiveness of the proposed PIM-DMT-DD system are investigated via theoretical analyses and simulation studies.

Factors Affecting University Teaching Team Effectiveness in Detached Working Environments

ERIC Educational Resources Information Center

Bennett, Roger; Kane, Suzanne

2014-01-01

This paper presents the outcomes of a study of the factors that contribute to teaching team effectiveness in situations where team members rarely meet face to face. Academic faculty within a university Business School were asked to report the degrees to which they believed that the module teaching teams to which they belonged contained members who…

Aternating current photovoltaic building block

DOEpatents

Bower, Ward Issac; Thomas, Michael G.; Ruby, Douglas S.

2004-06-15

A modular apparatus for and method of alternating current photovoltaic power generation comprising via a photovoltaic module, generating power in the form of direct current; and converting direct current to alternating current and exporting power via one or more power conversion and transfer units attached to the module, each unit comprising a unitary housing extending a length or width of the module, which housing comprises: contact means for receiving direct current from the module; one or more direct current-to-alternating current inverters; an alternating current bus; and contact means for receiving alternating current from the one or more inverters.

12 CFR 211.8 - Investments and activities abroad.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 2 2011-01-01 2011-01-01 false Investments and activities abroad. 211.8... Investments and activities abroad. (a) General policy. Activities abroad, whether conducted directly or... of a member bank is deemed to be an investor. (b) Direct investments by member banks. A member bank's...

An assessment strategy for proposals of engineering projects in the Bachelor of Biomedical Engineering Curriculum at Universidad Autónoma Metropolitana-Iztapalapa.

PubMed

Castañeda-Villa, N; Jiménez-González, A; Ortiz-Posadas, M R

2015-08-01

Since 1974, the Bachelor of Biomedical Engineering Program (BBME) is offered at Universidad Autónoma Metropolitana-Iztapalapa, in Mexico City. By design, it must be completed in four years (12 trimesters) and, in the latter three, the senior students work on a BME project, which is done by completing three modules: Project Seminar (PS), Project on BME I and Project on BME II. In the PS module, the student must find a problem of interest in the BME field and suggest a solution through the development of an Engineering Project Proposal (EPP). Currently, the module is being taught by two faculty members of the BBME, who instruct students on how to develop their EPPs and evaluate their progress by reviewing a number of EPPs during the trimester. This generates a huge workload for the module instructors, which makes it necessary to involve more faculty members trimester-to-trimester (i.e. every 12 weeks) and, therefore, to create a set of systematic guidelines that ease the evaluation process for new instructors. Hence, the purpose of this paper is to present an assessment strategy (in the form of an assessment matrix) for the PS module as well as some preliminary results after two trimesters of its implementation.

KSC-99pp1375

NASA Image and Video Library

1999-12-02

KENNEDY SPACE CENTER, FLA. -- Looking over a Pressurized Mating Adapter (PMA-3) in the Space Station Processing Facility are Arne Aamodt, with Johnson Space Center, Yuriy Vladimirovich Usachev and Susan J. Helms. Usachev and Helms are two members of the STS-102 crew, who will be staying on the International Space Station (ISS). The third crew member is James S. Voss. They have been designated the Expedition II crew. Mission STS-102 also will be carrying the Leonardo Multi-Purpose Logistics Module (MPLM) to the ISS. The Leonardo will be filled with equipment and supplies to outfit the U.S. laboratory module, which will have been carried to the ISS on a preceding Shuttle flight. In order to function as an attached station module as well as a cargo transport, logistics modules (there are three) also include components that provide some life support, fire detection and suppression, electrical distribution and computer functions. Eventually, the modules also will carry refrigerator freezers for transporting experiment samples and food to and from the station. On the return of STS-102 to Earth, it will bring back the first crew on the station: Bill Shepherd, Sergei Krikalev and Yuri Gidzenko. STS-102 is scheduled to launch no earlier than Oct. 19, 2000, from Launch Pad 39A, Kennedy Space Center

KSC-99pp1378

NASA Image and Video Library

1999-12-02

KENNEDY SPACE CENTER, FLA. -- From a work stand in the Space Station Processing Facility, STS-102 crew members James S. Voss (left) and Yuriy Vladimirovich Usachev (right), of Russia, look over the Pressurized Mating Adapter (PMA-3). The PMA-3 is a component of the International Space Station (ISS). Voss and Usachev are two crew members who will be staying on the ISS as the Expedition II crew. The third is Susan J. Helms. Along with the crew, Mission STS-102 also will be carrying the Leonardo Multi-Purpose Logistics Module (MPLM) to the ISS. The Leonardo will be filled with equipment and supplies to outfit the U.S. laboratory module, which will have been carried to the ISS on a preceding Shuttle flight. In order to function as an attached station module as well as a cargo transport, logistics modules (there are three) also include components that provide some life support, fire detection and suppression, electrical distribution and computer functions. Eventually, the modules also will carry refrigerator freezers for transporting experiment samples and food to and from the station. On the return of STS-102 to Earth, it will bring back the first crew on the station: Bill Shepherd, Sergei Krikalev and Yuri Gidzenko. STS-102 is scheduled to launch no earlier than Oct. 19, 2000, from Launch Pad 39A, Kennedy Space Center

Continuities and Discontinuities in Accounting Systems: 1998 Survey of ASBO International Membership in North America.

ERIC Educational Resources Information Center

Henstock, Thomas F.

1999-01-01

Based on a survey of Association of School Business Officials members, this article focuses on 10 accounting system modules and how respondents felt about their system's operation and integration. Results showed solid continuity among general ledger, accounts payable, and payroll modules and discontinuities among fixed-asset and student accounting…

Apollo 12 crewmen participate in water egress training

NASA Technical Reports Server (NTRS)

1969-01-01

A member of the prime crew of the Apollo 12 lunar landing mission is hoisted up to a U.S. Coast Guard helicopter during water egress training in the Gulf of Mexico. Participating in the training were Astronauts Charles Conrad Jr., commander; Richard F. Gordon Jr., command module pilot; and Alan L. Bean, lunar module pilot.

Sturckow in the Node 1/Unity module

NASA Image and Video Library

1999-01-11

STS088-359-003 (4-15 Dec. 1998) --- Astronaut Frederick W. ?Rick? Sturckow, pilot, holds up a panel while putting final touches on the interior of Node 1 or Unity. Sturckow, who had been working with a battery-powered tool, was joined by other crew members in preparing the module for its International Space Station (ISS) duty.

Voss in Service Module

NASA Image and Video Library

2001-03-31

ISS002-E-5084 (31 March 2001) --- Astronaut James S. Voss, Expedition Two flight engineer, floats in the Zvezda Service Module onboard the International Space Station (ISS). Voss, along with astronaut Susan J. Helms and cosmonaut Yury V. Usachev of Rosaviakosmos, recently replaced the initial three-member crew onboard the orbital outpost. The image was taken with a digital still camera.

Mobile learning module improves knowledge of medical shock for forward surgical team members.

PubMed

Schulman, Carl I; Garcia, George D; Wyckoff, Mary M; Duncan, Robert C; Withum, Kelly F; Graygo, Jill

2012-11-01

Acute trauma care is characterized by dynamic situations that require adequate preparation to ensure success for military health professionals. The use of mobile learning in this environment can provide a solution that standardizes education and replaces traditional didactic lectures. A comparative evaluation with a pre-post test design regarding medical shock was delivered via either a didactic lecture or a mobile learning video module to U.S. Army Forward Surgical Team (FST) members. Participants completed a pretest, were randomly assigned to treatment group by FST, and then completed the post-test and scenario assessment. One-hundred and thirteen FST members participated with 53 in the mobile learning group and 60 in the lecture group (control). The percent mean score for the mobile learning group increased from 43.6 to 70 from pretest to post-test, with a scenario mean score of M = 56.2. The percent mean score for the control group increased from 41.5 to 72.5, with a scenario mean score of M = 59.7. The two-way analysis of variance mean score difference was 26.4 for the mobile learning group and 31.0 for the control, F = 2.18, (p = 0.14). Mobile learning modules, coupled with a structured assessment, have the potential to improve educational experiences in civilian and military settings.

Taxel-addressable matrix of vertical nanowire piezotronic transistors

DOEpatents

Wang, Zhong Lin; Wu, Wenzhuo; Wen, Xiaonan

2015-05-05

A tactile sensing matrix includes a substrate, a first plurality of elongated electrode structures, a plurality of vertically aligned piezoelectric members, an insulating layer infused into the piezoelectric members and a second plurality of elongated electrode structures. The first plurality of elongated electrode structures is disposed on the substrate along a first orientation. The vertically aligned piezoelectric members is disposed on the first plurality of elongated electrode structures and form a matrix having columns of piezoelectric members disposed along the first orientation and rows of piezoelectric members disposed along a second orientation that is transverse to the first orientation. The second plurality of elongated electrode structures is disposed on the insulating layer along the second orientation. The elongated electrode structures form a Schottky contact with the piezoelectric members. When pressure is applied to the piezoelectric members, current flow therethrough is modulated.

Examination of the Structural Response of the Orion European Service Module to Reverberant and Direct Field Acoustic Testing

NASA Technical Reports Server (NTRS)

McNelis, Mark E.; Hughes, William O.; Larko, Jeffrey M.; Bittinger, Samantha A.; Le-Plenier, Cyprien; Fogt, Vincent A.; Ngan, Ivan; Thirkettle, Anthony C.; Skinner, Mitch; Larkin, Paul

2017-01-01

The NASA Orion Multi-Purpose Crew Vehicle (MPCV), comprised of the Service Module, the Crew Module, and the Launch Abort System, is the next generation human spacecraft designed and built for deep space exploration. Orion will launch on NASAs new heavy-lift rocket, the Space Launch System. The European Space Agency (ESA) is responsible for providing the propulsion sub-assembly of the Service Module to NASA, called the European Service Module (ESM). The ESM is being designed and built by Airbus Safran Launchers for ESA. Traditionally, NASA has utilized reverberant acoustic testing for qualification of spaceflight hardware. The ESM Structural Test Article (E-STA) was tested at the NASA Plum Brook Stations (PBS) Reverberant Acoustic Test Facility in April-May 2016. However, Orion is evaluating an alternative acoustic test method, using direct field acoustic excitation, for the MPCVs Service Module and Crew Module. Lockheed Martin is responsible for the Orion proof-of-concept direct field acoustic test program. The E-STA was exposed to direct field acoustic testing at NASA PBS in February 2017. This paper compares the dynamic response of the E-STA structure and its components to both the reverberant and direct field acoustic test excitations. Advantages and disadvantages of direct field acoustic test excitation method are discussed.

Essentially semismall Quasi-Dedekind module relative to a module

NASA Astrophysics Data System (ADS)

Hussain, Mukdad Q.

2018-05-01

Let R be associative ring with identity and M be a unitary R-module. In this paper study the direct summand of essentially semismall quasi-Dedekind module and prove that the direct sum of essentially semismall quasi-Dedekind modules need not be essentially semismall quasi-Dedekind and give the definition of essentially semismall quasi-Dedekind relative to a module with some examples, also give some of their basic properties and some examples that illustrate these properties.

Climate Science across the Liberal Arts Curriculum at Gustavus Adolphus College

NASA Astrophysics Data System (ADS)

Bartley, J. K.; Triplett, L.; Dontje, J.; Huber, T.; Koomen, M.; Jeremiason, J.; La Frenierre, J.; Niederriter, C.; Versluis, A.

2014-12-01

The human and social dimensions of climate change are addressed in courses in humanities, social sciences, and arts disciplines. However, faculty members in these disciplines are not climate science experts and thus may feel uncomfortable discussing the science that underpins our understanding of climate change. In addition, many students are interested in the connections between climate change and their program of study, but not all students take courses that address climate science as a principal goal. At Gustavus Adolphus College, the Climate Science Project aims to help non-geoscience faculty introduce climate science content in their courses in order to increase climate science literacy among students and inform discussions of the implications of climate change. We assembled an interdisciplinary team of faculty with climate science expertise to develop climate science modules for use in non-geoscience courses. Faculty from the social sciences, humanities, arts, education, and natural sciences attended workshops in which they developed plans to include climate science in their courses. Based on these workshops, members of the development team created short modules for use by participating faculty that introduce climate science concepts to a non-specialist audience. Each module was tested and modified prior to classroom implementation by a team of faculty and geoscience students. Faculty and student learning are assessed throughout the process, and participating faculty members are interviewed to improve the module development process. The Climate Science Project at Gustavus Adolphus College aims to increase climate science literacy in both faculty members and students by creating accessible climate science content and supporting non-specialist faculty in learning key climate science concepts. In this way, climate science becomes embedded in current course offerings, including non-science courses, reaching many more students than new courses or enhanced content in the geosciences can reach. In addition, this model can be adopted by institutions with limited geoscience course offerings to increase geoscience literacy among a broad cross-section of students.

Expedition 30 crewmembers in the SM

NASA Image and Video Library

2012-03-24

ISS030-E-173969 (24 March 2012) --- Expedition 30 crew members are pictured in the Zvezda Service Module of the International Space Station as they prepare to move to the appropriate Soyuz vehicles, due to the possibility that space debris could pass close to the station. Burbank, Shkaplerov and Ivanishin sheltered in the Soyuz TMA-22 spacecraft attached to the Poisk Mini-Research Module 2 (MRM2) while Kononenko, Kuipers and Pettit took to the Soyuz TMA-03M docked to the Rassvet Mini-Research Module 1 (MRM-1).

Starboard-Zenith (+YA, -ZA) side of Node 1/Unity and FGB/Zarya

NASA Image and Video Library

1998-12-13

STS088-703-019 (4-15 Dec. 1998) --- The U.S.-built Unity connecting module (bottom) and the Russian-built Zarya module are backdropped against the blackness of space in this 70mm photograph taken from the Space Shuttle Endeavour. After devoting the major portion of its mission time to various tasks to ready the two docked modules for their International Space Station (ISS) roles, the six-member STS-88 crew released the tandem and performed a fly-around survey of the hardware.

FXR and liver carcinogenesis

PubMed Central

Huang, Xiong-fei; Zhao, Wei-yu; Huang, Wen-dong

2015-01-01

Farnesoid X receptor (FXR) is a member of the nuclear receptor family and a ligand-modulated transcription factor. In the liver, FXR has been considered a multi-functional cell protector and a tumor suppressor. FXR can suppress liver carcinogenesis via different mechanisms: 1) FXR maintains the normal liver metabolism of bile acids, glucose and lipids; 2) FXR promotes liver regeneration and repair after injury; 3) FXR protects liver cells from death and enhances cell survival; 4) FXR suppresses hepatic inflammation, thereby preventing inflammatory damage; and 5) FXR can directly increase the expression of some tumor-suppressor genes and repress the transcription of several oncogenes. However, inflammation and epigenetic silencing are known to decrease FXR expression during tumorigenesis. The reactivation of FXR function in the liver may be a potential therapeutic approach for patients with liver cancer. PMID:25500874

Allosteric Inhibition of the Neuropeptidase Neurolysin*

PubMed Central

Hines, Christina S.; Ray, Kallol; Schmidt, Jack J.; Xiong, Fei; Feenstra, Rolf W.; Pras-Raves, Mia; de Moes, Jan Peter; Lange, Jos H. M.; Melikishvili, Manana; Fried, Michael G.; Mortenson, Paul; Charlton, Michael; Patel, Yogendra; Courtney, Stephen M.; Kruse, Chris G.; Rodgers, David W.

2014-01-01

Neuropeptidases specialize in the hydrolysis of the small bioactive peptides that play a variety of signaling roles in the nervous and endocrine systems. One neuropeptidase, neurolysin, helps control the levels of the dopaminergic circuit modulator neurotensin and is a member of a fold group that includes the antihypertensive target angiotensin converting enzyme. We report the discovery of a potent inhibitor that, unexpectedly, binds away from the enzyme catalytic site. The location of the bound inhibitor suggests it disrupts activity by preventing a hinge-like motion associated with substrate binding and catalysis. In support of this model, the inhibition kinetics are mixed, with both noncompetitive and competitive components, and fluorescence polarization shows directly that the inhibitor reverses a substrate-associated conformational change. This new type of inhibition may have widespread utility in targeting neuropeptidases. PMID:25378390

KSC-2009-6517

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Bernardo Patti, right, head of International Space Station, Program Department, European Space Agency, or ESA, has a lot to smile about as he is photographed in front of the node 3 for the International Space Station following a ceremony transferring the ownership of the node from ESA to NASA. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

KSC-2009-6514

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, from left, Michael Suffredini, program manager, International Space Station, NASA; Secondino Brondolo, head of the Space Infrastructure, Thales Alenia Space Italy; and Bernardo Patti, head of International Space Station, Program Department, ESA, are photographed in front of node 3 for the International Space Station following a ceremony transferring the ownership of the node from the European Space Agency, or ESA, to NASA. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

Comic books carry health messages to rural children in Honduras.

PubMed

Vigano, O

1983-12-01

This article reports on the use of children as message carriers in a rural water and sanitation project in western Honduras. The Honduran Water and Sanitation Project represents the 1st such effort to have a specific health education component. It was decided to direct the education component toward children because of their important role in providing and handling drinking water and caring for younger members of the family. Rural primary schools surfaced as a potential channel of communication. The comic book format was selected because it is simple enough to be used in the schools without much training, economical to produce (US$0.30/copy), effective and attractive to children, and consistent with the Project's philosophy that dialogue and participation are essential components of health education. Each comic book contains a single-concept message, e.g., 1 cause of water contamination or a method of water purification. The 1st module was pretested in 3 rural schools. Following classroom study of the comic book, correct answers on 5 questions related to the comic book story increased from 59% to 80%. 95% of the children indicated that they liked the characters, and teachers expressed satisfaction with the materials. 1200 copies of the 1st module have been distributed to 30 rural schools, and production plans include 11 additional modules on topics such as prevention of water-related sickness and personal hygiene.

Intestinal Microbiota Modulates Gluten-Induced Immunopathology in Humanized Mice

PubMed Central

Galipeau, Heather J.; McCarville, Justin L.; Huebener, Sina; Litwin, Owen; Meisel, Marlies; Jabri, Bana; Sanz, Yolanda; Murray, Joseph A.; Jordana, Manel; Alaedini, Armin; Chirdo, Fernando G.; Verdu, Elena F.

2016-01-01

Celiac disease (CD) is an immune-mediated enteropathy triggered by gluten in genetically susceptible individuals. The recent increase in CD incidence suggests that additional environmental factors, such as intestinal microbiota alterations, are involved in its pathogenesis. However, there is no direct evidence of modulation of gluten-induced immunopathology by the microbiota. We investigated whether specific microbiota compositions influence immune responses to gluten in mice expressing the human DQ8 gene, which confers moderate CD genetic susceptibility. Germ-free mice, clean specific-pathogen-free (SPF) mice colonized with a microbiota devoid of opportunistic pathogens and Proteobacteria, and conventional SPF mice that harbor a complex microbiota that includes opportunistic pathogens were used. Clean SPF mice had attenuated responses to gluten compared to germ-free and conventional SPF mice. Germ-free mice developed increased intraepithelial lymphocytes, markers of intraepithelial lymphocyte cytotoxicity, gliadin-specific antibodies, and a proinflammatory gliadin-specific T-cell response. Antibiotic treatment, leading to Proteobacteria expansion, further enhanced gluten-induced immunopathology in conventional SPF mice. Protection against gluten-induced immunopathology in clean SPF mice was reversed after supplementation with a member of the Proteobacteria phylum, an enteroadherent Escherichia coli isolated from a CD patient. The intestinal microbiota can both positively and negatively modulate gluten-induced immunopathology in mice. In subjects with moderate genetic susceptibility, intestinal microbiota changes may be a factor that increases CD risk. PMID:26456581

Opportunities to Learn about Europe at School. A Comparative Analysis among European Adolescents in 21 European Member States

ERIC Educational Resources Information Center

Verhaegen, Soetkin; Hooghe, Marc; Meeusen, Cecil

2013-01-01

In this article, we investigate the relationship between different learning methods and the formation of European identity among adolescents. The analysis is based on the European module of the International Civic and Citizenship Education Study (2009), with 70,502 respondents in 21 European member states. The results show that offering…

Hadfield plays guitar in the Cupola Module

NASA Image and Video Library

2012-12-25

ISS034-E-009787 (25 Dec. 2012) --- Canadian Space Agency astronaut Chris Hadfield strums his guitar in the International Space Station's Cupola on Dec. 25, 2012. Hadfield, a long-time member of an astronaut band called Max Q, later joined with the other five Expedition 34 crew members in a more spacious location to provide an assortment of Christmas carols for the public.

Hadfield plays guitar in the Cupola Module

NASA Image and Video Library

2012-12-25

ISS034-E-009799 (25 Dec. 2012) --- Canadian Space Agency astronaut Chris Hadfield strums his guitar in the International Space Station's Cupola on Dec. 25, 2012. Hadfield, a long-time member of an astronaut band called Max Q, later joined with the other five Expedition 34 crew members in a more spacious location to provide an assortment of Christmas carols for the public.

Hadfield plays guitar in the Cupola Module

NASA Image and Video Library

2012-12-25

ISS034-E-010295 (25 Dec. 2012) --- Canadian Space Agency astronaut Chris Hadfield strums his guitar in the International Space Station's Cupola on Dec. 25, 2012. Hadfield, a long-time member of an astronaut band called Max Q, later joined with the other five Expedition 34 crew members in a more spacious location to provide an assortment of Christmas carols for the public.

MicroRNA cluster miR-17-92 regulates multiple functionally related voltage-gated potassium channels in chronic neuropathic pain

PubMed Central

Sakai, Atsushi; Saitow, Fumihito; Maruyama, Motoyo; Miyake, Noriko; Miyake, Koichi; Shimada, Takashi; Okada, Takashi; Suzuki, Hidenori

2017-01-01

miR-17-92 is a microRNA cluster with six distinct members. Here, we show that the miR-17-92 cluster and its individual members modulate chronic neuropathic pain. All cluster members are persistently upregulated in primary sensory neurons after nerve injury. Overexpression of miR-18a, miR-19a, miR-19b and miR-92a cluster members elicits mechanical allodynia in rats, while their blockade alleviates mechanical allodynia in a rat model of neuropathic pain. Plausible targets for the miR-17-92 cluster include genes encoding numerous voltage-gated potassium channels and their modulatory subunits. Single-cell analysis reveals extensive co-expression of miR-17-92 cluster and its predicted targets in primary sensory neurons. miR-17-92 downregulates the expression of potassium channels, and reduced outward potassium currents, in particular A-type currents. Combined application of potassium channel modulators synergistically alleviates mechanical allodynia induced by nerve injury or miR-17-92 overexpression. miR-17-92 cluster appears to cooperatively regulate the function of multiple voltage-gated potassium channel subunits, perpetuating mechanical allodynia. PMID:28677679

View of damaged Apollo 13 Service Module from the Lunar/Command Modules

NASA Image and Video Library

1970-04-17

AS13-58-8458 (17 April 1970) --- This view of the severely damaged Apollo 13 Service Module (SM) was photographed from the Lunar Module/Command Module (LM/CM) following SM jettisoning. An entire SM panel was blown away by the apparent explosion of oxygen tank number two. Two of the three fuel cells are visible at the forward portion of the opening. The hydrogen tanks are located in Sector 4 of the Apollo 13 SM. The apparent rupture of the oxygen tank caused the Apollo 13 crew members to use the LM as a "lifeboat." The LM was jettisoned just prior to Earth re-entry by the CM.

Overview of radar intra-pulse modulation recognition

NASA Astrophysics Data System (ADS)

Zang, Hanlin; Li, Yanling

2018-05-01

This paper introduces the current radar intra-pulse modulation method, describes the status quo and development direction of the intentional modulation and unintentional modulation in the pulse, and summarizes the existing problems and prospects for the future. Looking forward to the future, and providing a reference direction for the research on radar signal recognition in the next step.

Connections for solid oxide fuel cells

DOEpatents

Collie, Jeffrey C.

1999-01-01

A connection for fuel cell assemblies is disclosed. The connection includes compliant members connected to individual fuel cells and a rigid member connected to the compliant members. Adjacent bundles or modules of fuel cells are connected together by mechanically joining their rigid members. The compliant/rigid connection permits construction of generator fuel cell stacks from basic modular groups of cells of any desired size. The connections can be made prior to installation of the fuel cells in a generator, thereby eliminating the need for in-situ completion of the connections. In addition to allowing pre-fabrication, the compliant/rigid connections also simplify removal and replacement of sections of a generator fuel cell stack.

Directional and Non-directional Hypothesis Testing: A Survey of SIG Members, Journals, and Textbooks.

ERIC Educational Resources Information Center

McNeil, Keith

The use of directional and nondirectional hypothesis testing was examined from the perspectives of textbooks, journal articles, and members of editorial boards. Three widely used statistical texts were reviewed in terms of how directional and nondirectional tests of significance were presented. Texts reviewed were written by: (1) D. E. Hinkle, W.…

A Rab5 GTPase module is important for autophagosome closure

PubMed Central

Lipatova, Zhanna; Sun, Dan; Zhu, Xiaolong; Li, Rui; Wu, Zulin; You, Weiming; Cong, Xiaoxia; Zhou, Yiting; Gyurkovska, Valeriya; Liu, Yutao; Li, Qunli; Li, Wenjing; Cheng, Jie; Segev, Nava

2017-01-01

In the conserved autophagy pathway, the double-membrane autophagosome (AP) engulfs cellular components to be delivered for degradation in the lysosome. While only sealed AP can productively fuse with the lysosome, the molecular mechanism of AP closure is currently unknown. Rab GTPases, which regulate all intracellular trafficking pathways in eukaryotes, also regulate autophagy. Rabs function in GTPase modules together with their activators and downstream effectors. In yeast, an autophagy-specific Ypt1 GTPase module, together with a set of autophagy-related proteins (Atgs) and a phosphatidylinositol-3-phosphate (PI3P) kinase, regulates AP formation. Fusion of APs and endosomes with the vacuole (the yeast lysosome) requires the Ypt7 GTPase module. We have previously shown that the Rab5-related Vps21, within its endocytic GTPase module, regulates autophagy. However, it was not clear which autophagy step it regulates. Here, we show that this module, which includes the Vps9 activator, the Rab5-related Vps21, the CORVET tethering complex, and the Pep12 SNARE, functions after AP expansion and before AP closure. Whereas APs are not formed in mutant cells depleted for Atgs, sealed APs accumulate in cells depleted for the Ypt7 GTPase module members. Importantly, depletion of individual members of the Vps21 module results in a novel phenotype: accumulation of unsealed APs. In addition, we show that Vps21-regulated AP closure precedes another AP maturation step, the previously reported PI3P phosphatase-dependent Atg dissociation. Our results delineate three successive steps in the autophagy pathway regulated by Rabs, Ypt1, Vps21 and Ypt7, and provide the first insight into the upstream regulation of AP closure. PMID:28934205

Linearized electrooptic polymeric directional coupler modulator

NASA Astrophysics Data System (ADS)

Hung, Yu-Chueh

External linearized modulators are required in high-performance analog optical communication systems since the performance of conventional modulators, such as Mach-Zehnder modulators, are degraded by distortions by the nonlinearity of their transfer functions. Various linearization schemes have been proposed to increase the dynamic range of an analog optical link. Most of the optical schemes involve multiple Mach-Zehnder modulators, either in parallel or series configuration, incorporated with strict balance of RF and bias control. This is a significant challenge when it comes to practical implementation. In this dissertation, a linearized two-section directional coupler modulator made from electrooptic polymer is presented. The coupling coefficient of each section is tailored by properly tuning the refractive index contrast, which can be easily employed using the photobleaching technique in polymer technology. A two-tone test was performed to evaluate the linearity of the modulator and the spur-free dynamic range shows a 7.5 dB improvement compared to a conventional Mach-Zehnder modulator. This scheme avoids multiple modulators or complicated modulation synchronization and demonstrates a compact design in real implementation. Most of the linearization schemes up to date consider only the direct detection mode of operation. However, the RF output characteristics at the detection side are determined differently by various system parameters if a coherent link is implemented instead. Therefore, different considerations of linearization have to be examined for this kind of application. In the second part of this dissertation, the impact of various modulation scenarios on the system performance of an analog coherent optical link will be addressed. It will be shown that a directional coupler modulator is better suited at increasing the dynamic range in coherent optical links. Specific designs of a directional coupler modulator shows an SFDR improvement of 20 dB compared to a Mach-Zehnder modulator. This new type of device can be easily fabricated using photobleaching technique in eletrooptic polymer and can be utilized in various applications.

Lattice strain of osmium diboride under high pressure and nonhydrostatic stress

NASA Astrophysics Data System (ADS)

Kavner, Abby; Weinberger, Michelle B.; Shahar, Anat; Cumberland, Robert W.; Levine, Jonathan B.; Kaner, Richard B.; Tolbert, Sarah H.

2012-07-01

The lattice strain behavior of osmium diboride—a member of a group of third-row transition metal borides associated with hard/superhard behavior—has been studied using radial diffraction in a diamond anvil cell under high pressure and non-hydrostatic stress. We interpret the average values of the measured lattice strains as a lower-bound to the lattice-plane dependent yield strengths using existing estimates for the elastic constants of OsB2, with a yield strength of 11 GPa at 27.5 GPa of hydrostatic pressure. The measured differential lattice strains show significant plane-dependent anisotropy, with the (101) lattice plane showing the largest differential strain and the (001) lattice plane showing the least strain. At the highest pressure, the a-axis develops a larger compressive strain and supports a larger differential strain than either the b or c axes. This causes an increase in the c/a ratio and a decrease in the a/b ratio especially in the maximum stress direction. The large strength anisotropy of this material points to possible ways to modulate directional mechanical properties by taking advantage of the interplay between aggregate polycrystalline texture with directional mechanical properties.

DNA repair and aging: the impact of the p53 family.

PubMed

Nicolai, Sara; Rossi, Antonello; Di Daniele, Nicola; Melino, Gerry; Annicchiarico-Petruzzelli, Margherita; Raschellà, Giuseppe

2015-12-01

Cells are constantly exposed to endogenous and exogenous factors that threaten the integrity of their DNA. The maintenance of genome stability is of paramount importance in the prevention of both cancer and aging processes. To deal with DNA damage, cells put into operation a sophisticated and coordinated mechanism, collectively known as DNA damage response (DDR). The DDR orchestrates different cellular processes, such as DNA repair, senescence and apoptosis. Among the key factors of the DDR, the related proteins p53, p63 and p73, all belonging to the same family of transcription factors, play multiple relevant roles. Indeed, the members of this family are directly involved in the induction of cell cycle arrest that is necessary to allow the cells to repair. Alternatively, they can promote cell death in case of prolonged or irreparable DNA damage. They also take part in a more direct task by modulating the expression of core factors involved in the process of DNA repair or by directly interacting with them. In this review we will analyze the fundamental roles of the p53 family in the aging process through their multifaceted function in DDR.

DNA repair and aging: the impact of the p53 family

PubMed Central

Nicolai, Sara; Rossi, Antonello; Di Daniele, Nicola; Melino, Gerry; Annicchiarico-Petruzzelli, Margherita; Raschellà, Giuseppe

2015-01-01

Cells are constantly exposed to endogenous and exogenous factors that threaten the integrity of their DNA. The maintenance of genome stability is of paramount importance in the prevention of both cancer and aging processes. To deal with DNA damage, cells put into operation a sophisticated and coordinated mechanism, collectively known as DNA damage response (DDR). The DDR orchestrates different cellular processes, such as DNA repair, senescence and apoptosis. Among the key factors of the DDR, the related proteins p53, p63 and p73, all belonging to the same family of transcription factors, play multiple relevant roles. Indeed, the members of this family are directly involved in the induction of cell cycle arrest that is necessary to allow the cells to repair. Alternatively, they can promote cell death in case of prolonged or irreparable DNA damage. They also take part in a more direct task by modulating the expression of core factors involved in the process of DNA repair or by directly interacting with them. In this review we will analyze the fundamental roles of the p53 family in the aging process through their multifaceted function in DDR. PMID:26668111

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Soichi Noguchi is happy to be back at KSC after arriving aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Soichi Noguchi is happy to be back at KSC after arriving aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Commander Eileen Collins is pleased to be back at KSC after arriving aboard a T-38 jet aircraft. She and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver to the Space Station the external stowage platform and the Multi-Purpose Logistics Module with supplies and equipment.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Commander Eileen Collins is pleased to be back at KSC after arriving aboard a T-38 jet aircraft. She and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver to the Space Station the external stowage platform and the Multi-Purpose Logistics Module with supplies and equipment.

KENNEDY SPACE CENTER, FLA. - STS-114 Pilot Jim Kelly is pleased to be back at KSC after arriving aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - STS-114 Pilot Jim Kelly is pleased to be back at KSC after arriving aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Andrew Thomas is pleased to be back at KSC after arriving aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver to the Space Station the external stowage platform and the Multi-Purpose Logistics Module with supplies and equipment.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Andrew Thomas is pleased to be back at KSC after arriving aboard a T-38 jet aircraft. He and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver to the Space Station the external stowage platform and the Multi-Purpose Logistics Module with supplies and equipment.

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Wendy Lawrence is pleased to be back at KSC after arriving aboard a T-38 jet aircraft. She and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Wendy Lawrence is pleased to be back at KSC after arriving aboard a T-38 jet aircraft. She and other crew members are at the Center for familiarization activities with equipment. The mission is Logistics Flight 1, scheduled to deliver the Multi-Purpose Logistics Module carrying supplies and equipment to the Space Station and the external stowage platform.

Crew Training - Apollo X (Apollo Mission Simulator [AMS])

NASA Image and Video Library

1969-04-05

S69-32787 (3 April 1969) --- Two members of the Apollo 10 prime crew participate in simulation activity at the Kennedy Space Center during preparations for their scheduled lunar orbit mission. Astronaut Thomas P. Stafford, commander, is in the background; and in the foreground is astronaut Eugene A. Cernan, lunar module pilot. The two crewmen are in the Lunar Module Mission Simulator.

Food Lab - Technician - MSC

NASA Image and Video Library

1970-04-09

S70-34848 (11 April 1970) --- Astronaut James A. Lovell Jr., commander for NASA's Apollo 13 mission, undergoes space suit checks a few hours before launch. Other members of the crew are astronauts Fred W. Haise Jr., lunar module pilot, and John L. Swigert Jr., command module pilot. Swigert replaced astronaut Thomas K. Mattingly II when it was learned he had been exposed to measles.

The PLIN4 variant rs8887 modulates obesity related phenotypes in humans through creation of a novel miR-522 seed site

USDA-ARS?s Scientific Manuscript database

PLIN4 is a member of the PAT family of lipid storage droplet (LSD) proteins. Associations between seven single nucleotide polymorphisms (SNPs) at human PLIN4 with obesity related phenotypes were investigated using meta-analysis followed by a determination if these phenotypes are modulated by intera...

Vocational Training in Europe: Towards a Modular Form? Discussion Paper. CEDEFOP Panorama. First Edition.

ERIC Educational Resources Information Center

Sellin, Burkart

Discussion of whether and to what extent initial vocational training and adult education in European Community (EC) member countries can assume a modular form hinges on the issue of the module as an organizational principle. In such a context, modules are viewed not as closed teaching and learning units but rather as integral parts of a more…

"Do We Really Need to Know This?" The Challenge of Developing a Global Learning Module for Trainee Teachers

ERIC Educational Resources Information Center

Scoffham, Stephen

2013-01-01

This article explores some of the challenges of developing a module on global learning with primary initial teacher education (ITE) students studying at a UK university. The research, which employed a mixed methodology, involved around 550 students and eight members of staff over a three-year period. The findings indicate that many students…

KSC-07pd2833

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, STS-123 crew members get a close look at hardware related to the mission. They are at the center for a crew equipment interface test, which allows familiarization with equipment they will use during the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

KSC-07pd2832

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, STS-123 crew members get a close look at hardware related to the mission. They are at the center for a crew equipment interface test, which allows familiarization with equipment they will use during the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

KSC-07pd2831

NASA Image and Video Library

2007-10-11

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility at NASA's Kennedy Space Center, STS-123 crew members get a close look at hardware related to the mission. They are at the center for a crew equipment interface test, which allows familiarization with equipment they will use during the mission. Crew members are Commander Dominic Gorie, Pilot Gregory Johnson and Mission Specialists Richard Linnehan, Takao Doi, Robert Behnken, Gerrett Reisman and Michael Foreman. Doi represents the Japan Aerospace Exploration Agency. Reisman will remain on the space station after the mission as a flight engineer for Expedition 16. STS-123 will carry and install one of the components of the Japanese Experiment Module, or JEM. Known as Kibo, the JEM comprises six components: two research facilities -- the Pressurized Module and Exposed Facility; a Logistics Module attached to each of them; a Remote Manipulator System; and an Inter-Orbit Communication System unit. The various components of JEM will be assembled in space over the course of three space shuttle missions. The first of those three missions, STS-123, will carry the Experiment Logistics Module Pressurized Section aboard the space shuttle Endeavour, targeted for launch in February 2008. Photo credit: NASA/Dimitrios Gerondidakis

Self-Directed Digital Learning: When Do Dental Students Study?

PubMed

Jackson, Tate H; Zhong, James; Phillips, Ceib; Koroluk, Lorne D

2018-04-01

The Growth and Development (G&D) curriculum at the University of North Carolina at Chapel Hill School of Dentistry uses self-directed web-based learning modules in the place of lectures and includes scheduled self-study times during the 8 am-5 pm school hours. The aim of this study was to use direct observation to evaluate dental students' access patterns with the self-directed, web-based learning modules in relation to planned self-study time allocated across the curriculum, proximity to course examinations, and course performance. Module access for all 80 students in the DDS Class of 2014 was recorded for date and time across the four G&D courses. Module access data were used to determine likelihood of usage during scheduled time and frequency of usage in three timeframes: >7, 3 to 7, and 0 to 2 days before the final exam. The results showed a statistically significant difference in the likelihood of module access during scheduled time across the curriculum (p<0.0001). Among the students, 64% accessed modules at least once during scheduled time in G&D1, but only 10%, 19%, and 18% in G&D2, G&D3, and G&D4, respectively. For all courses, the proportion of module accesses was significantly higher 0-2 days before an exam compared to the other two timeframes. Module access also differed significantly within each timeframe across all four courses (p<0.001). There was no association between module access and course performance. In this non-traditional, non-lecture, self-directed curriculum, students rarely accessed learning modules during syllabus-budgeted self-study time and accessed modules more frequently as course exams approached.

Sensitivity of human auditory cortex to rapid frequency modulation revealed by multivariate representational similarity analysis.

PubMed

Joanisse, Marc F; DeSouza, Diedre D

2014-01-01

Functional Magnetic Resonance Imaging (fMRI) was used to investigate the extent, magnitude, and pattern of brain activity in response to rapid frequency-modulated sounds. We examined this by manipulating the direction (rise vs. fall) and the rate (fast vs. slow) of the apparent pitch of iterated rippled noise (IRN) bursts. Acoustic parameters were selected to capture features used in phoneme contrasts, however the stimuli themselves were not perceived as speech per se. Participants were scanned as they passively listened to sounds in an event-related paradigm. Univariate analyses revealed a greater level and extent of activation in bilateral auditory cortex in response to frequency-modulated sweeps compared to steady-state sounds. This effect was stronger in the left hemisphere. However, no regions showed selectivity for either rate or direction of frequency modulation. In contrast, multivoxel pattern analysis (MVPA) revealed feature-specific encoding for direction of modulation in auditory cortex bilaterally. Moreover, this effect was strongest when analyses were restricted to anatomical regions lying outside Heschl's gyrus. We found no support for feature-specific encoding of frequency modulation rate. Differential findings of modulation rate and direction of modulation are discussed with respect to their relevance to phonetic discrimination.

Coordination of NF-kappaB and NFAT antagonism by the forkhead transcription factor Foxd1.

PubMed

Lin, Ling; Peng, Stanford L

2006-04-15

Forkhead transcription factors play critical roles in the maintenance of immune homeostasis. In this study, we demonstrate that this regulation most likely involves intricate interactions between the forkhead family members and inflammatory transcription factors: the forkhead member Foxd1 coordinates the regulation of the activity of two key inflammatory transcription factors, NF-AT and NF-kappaB, with Foxd1 deficiency resulting in multiorgan, systemic inflammation, exaggerated Th cell-derived cytokine production, and T cell proliferation in autologous MLRs. Foxd1-deficient T cells possess increased activity of both NF-AT and NF-kappaB: the former correlates with the ability of Foxd1 to regulate casein kinase 1, an NF-AT inhibitory kinase; the latter with the ability of Foxd1 to regulate Foxj1, which regulates the NF-kappaB inhibitory subunit IkappaB beta. Thus, Foxd1 modulates inflammatory reactions and prevents autoimmunity by directly regulating anti-inflammatory regulators of the NF-AT pathway, and by coordinating the suppression of the NF-kappaB pathway via Foxj1. These findings indicate the presence of a general network of forkhead proteins that enforce T cell quiescence.

Officials welcome the arrival of the Japanese Experiment Module

NASA Image and Video Library

2007-04-17

In the Space Station Processing Facility, astronaut Takao Doi (left) and Commander Dominic Gorie pose in front of the Experiment Logistics Module Pressurized Section for the Japanese Experiment Module, or JEM, that recently arrived at Kennedy. Doi and Gorie are crew members for mission STS-123 that will deliver the logistics module to the International Space Station. Earlier, NASA and Japanese Aerospace and Exploration Agency (JAXA) officials welcomed the arrival of the module. The new International Space Station component arrived at Kennedy March 12 to begin preparations for its future launch on mission STS-123. It will serve as an on-orbit storage area for materials, tools and supplies. It can hold up to eight experiment racks and will attach to the top of another larger pressurized module.

Effect of Modulated Alternating and Direct Current Iontophoresis on Transdermal Delivery of Lidocaine Hydrochloride

PubMed Central

Banga, Ajay K.

2014-01-01

The objective of this study was to investigate the iontophoretic delivery of lidocaine hydrochloride through porcine skin and to compare the effects of modulated alternating and direct current iontophoresis. Continuous and modulated iontophoresis was applied for one hour and two hours (0-1 h and 4-5th h) using a 1% w/v solution of lidocaine hydrochloride. Tape stripping was done to quantify the amount of drug permeated into stratum corneum and skin extraction studies were performed to determine the amount of drug in stripped skin. Receptor was sampled and analyzed over predefined time periods. The amount of lidocaine delivered across porcine skin after modulated direct current iontophoresis for 2 h was 1069.87 ± 120.03 μg/sq·cm compared to 744.81 ± 125.41 μg/sq·cm after modulated alternating current iontophoresis for 2 h. Modulated direct current iontophoresis also enhanced lidocaine delivery by twelvefold compared to passive delivery as 91.27 ± 18.71 μg/sq·cm of lidocaine was delivered after passive delivery. Modulated iontophoresis enhanced the delivery of lidocaine hydrochloride across porcine skin compared to the passive delivery. Modulated alternating current iontophoresis for duration of 2 h at frequency of 1 kHz was found to be comparable to the continuous direct current iontophoresis for 1 h. PMID:24959580

Effect of modulated alternating and direct current iontophoresis on transdermal delivery of lidocaine hydrochloride.

PubMed

Bhatia, Gaurav; Banga, Ajay K

2014-01-01

The objective of this study was to investigate the iontophoretic delivery of lidocaine hydrochloride through porcine skin and to compare the effects of modulated alternating and direct current iontophoresis. Continuous and modulated iontophoresis was applied for one hour and two hours (0-1 h and 4-5th h) using a 1% w/v solution of lidocaine hydrochloride. Tape stripping was done to quantify the amount of drug permeated into stratum corneum and skin extraction studies were performed to determine the amount of drug in stripped skin. Receptor was sampled and analyzed over predefined time periods. The amount of lidocaine delivered across porcine skin after modulated direct current iontophoresis for 2 h was 1069.87 ± 120.03 μ g/sq · cm compared to 744.81 ± 125.41 μ g/sq · cm after modulated alternating current iontophoresis for 2 h. Modulated direct current iontophoresis also enhanced lidocaine delivery by twelvefold compared to passive delivery as 91.27 ± 18.71 μ g/sq · cm of lidocaine was delivered after passive delivery. Modulated iontophoresis enhanced the delivery of lidocaine hydrochloride across porcine skin compared to the passive delivery. Modulated alternating current iontophoresis for duration of 2 h at frequency of 1 kHz was found to be comparable to the continuous direct current iontophoresis for 1 h.

Soyuz Spacecraft

NASA Image and Video Library

2014-11-12

ISS038-E-000250 (12 Nov. 2013) --- The Russian Soyuz TMA-11M spacecraft dominates this image exposed by one of the Expedition 38 crew members aboard the International Space Station over Earth on Nov. 12. Now docked to the Rassvet or Mini-Research Module 1 (MRM-1), the spacecraft had delivered three crew members to the orbital outpost five days earlier, temporarily bringing the total population to nine aboard the station.

Studying p53 family proteins in yeast: Induction of autophagic cell death and modulation by interactors and small molecules

DOE Office of Scientific and Technical Information (OSTI.GOV)

Leão, Mariana; Gomes, Sara; Bessa, Cláudia

In this work, the yeast Saccharomyces cerevisiae was used to individually study human p53, p63 (full length and truncated forms) and p73. Using this cell system, the effect of these proteins on cell proliferation and death, and the influence of MDM2 and MDMX on their activities were analyzed. When expressed in yeast, wild-type p53, TAp63, ΔNp63 and TAp73 induced growth inhibition associated with S-phase cell cycle arrest. This growth inhibition was accompanied by reactive oxygen species production and autophagic cell death. Furthermore, they stimulated rapamycin-induced autophagy. On the contrary, none of the tested p53 family members induced apoptosis either permore » se or after apoptotic stimuli. As previously reported for p53, also TAp63, ΔNp63 and TAp73 increased actin expression levels and its depolarization, suggesting that ACT1 is also a p63 and p73 putative yeast target gene. Additionally, MDM2 and MDMX inhibited the activity of all tested p53 family members in yeast, although the effect was weaker on TAp63. Moreover, Nutlin-3a and SJ-172550 were identified as potential inhibitors of the p73 interaction with MDM2 and MDMX, respectively. Altogether, the yeast-based assays herein developed can be envisaged as a simplified cell system to study the involvement of p53 family members in autophagy, the modulation of their activities by specific interactors (MDM2 and MDMX), and the potential of new small molecules to modulate these interactions. - Highlights: • p53, p63 and p73 are individually studied in the yeast S. cerevisiae. • p53 family members induce ROS production, cell cycle arrest and autophagy in yeast. • p53 family members increase actin depolarization and expression levels in yeast. • MDM2 and MDMX inhibit the activity of p53 family members in yeast. • Yeast can be a useful tool to study the biology and drugability of p53, p63 and p73.« less

Directly Phase-Modulated Light Source

NASA Astrophysics Data System (ADS)

Yuan, Z. L.; Fröhlich, B.; Lucamarini, M.; Roberts, G. L.; Dynes, J. F.; Shields, A. J.

2016-07-01

The art of imparting information onto a light wave by optical signal modulation is fundamental to all forms of optical communication. Among many schemes, direct modulation of laser diodes stands out as a simple, robust, and cost-effective method. However, the simultaneous changes in intensity, frequency, and phase have prevented its application in the field of secure quantum communication. Here, we propose and experimentally demonstrate a directly phase-modulated light source which overcomes the main disadvantages associated with direct modulation and is suitable for diverse applications such as coherent communications and quantum cryptography. The source separates the tasks of phase preparation and pulse generation between a pair of semiconductor lasers leading to very pure phase states. Moreover, the cavity-enhanced electro-optic effect enables the first example of subvolt half-wave phase modulation at high signal rates. The source is compact, stable, and versatile, and we show its potential to become the standard transmitter for future quantum communication networks based on attenuated laser pulses.

Stacked vapor fed amtec modules

DOEpatents

Sievers, Robert K.

1989-01-01

The present invention pertains to a stacked AMTEC module. The invention includes a tubular member which has an interior. The member is comprised of a ion conductor that substantially conducts ions relative to electrons, preferably a beta"-alumina solid electrolyte, positioned about the interior. A porous electrode for conducting electrons and allowing sodium ions to pass therethrough, and wherein electrons and sodium ions recombine to form sodium is positioned about the beta"-alumina solid electrolyte. The electrode is operated at a temperature and a pressure that allows the recombined sodium to vaporize. Additionally, an outer current collector grid for distributing electrons throughout the porous electrode is positioned about and contacts the porous electrode. Also included in the invention is transporting means for transporting liquid sodium to the beta"-alumina solid electrolyte of the tubular member. A transition piece is positioned about the interior of the member and contacts the transporting means. The transition piece divides the member into a first cell and a second cell such that each first and second cell has a beta"-alumina solid electrolyte, a first and second porous electrode and a grid. The transition piece conducts electrons from the interior of the tubular member. There is supply means for supplying sodium to the transporting means. Preferably the supply means is a shell which surrounds the tubular member and is operated at a temperature such that the vaporized sodium condenses thereon. Returning means for returning the condensed sodium from the shell to the transporting means provides a continuous supply of liquid sodium to the transporting means. Also, there are first conducting means for conducting electric current from the transition piece which extends through the shell, and second conducting means for conducting electric current to the grid of the first cell which extends through the shell.

Tribbles pseudokinases: novel targets for chemical biology and drug discovery?

PubMed

Foulkes, Daniel M; Byrne, Dominic P; Bailey, Fiona P; Eyers, Patrick A

2015-10-01

Tribbles (TRIB) proteins are pseudokinase mediators of eukaryotic signalling that have evolved important roles in lipoprotein metabolism, immune function and cellular differentiation and proliferation. In addition, an evolutionary-conserved modulation of PI3K/AKT signalling pathways highlights them as novel and rather unusual pharmaceutical targets. The three human TRIB family members are uniquely defined by an acidic pseudokinase domain containing a 'broken' α C-helix and a MEK (MAPK/ERK)-binding site at the end of the putative C-lobe and a distinct C-terminal peptide motif that interacts directly with a small subset of cellular E3 ubiquitin ligases. This latter interaction drives proteasomal-dependent degradation of networks of transcription factors, whose rate of turnover determines the biological attributes of individual TRIB family members. Defining the function of individual Tribs has been made possible through evaluation of individual TRIB knockout mice, siRNA/overexpression approaches and genetic screening in flies, where the single TRIB gene was originally described 15 years ago. The rapidly maturing TRIB field is primed to exploit chemical biology approaches to evaluate endogenous TRIB signalling events in intact cells. This will help define how TRIB-driven protein-protein interactions and the atypical TRIB ATP-binding site, fit into cellular signalling modules in experimental scenarios where TRIB-signalling complexes remain unperturbed. In this mini-review, we discuss how small molecules can reveal rate-limiting signalling outputs and functions of Tribs in cells and intact organisms, perhaps serving as guides for the development of new drugs. We predict that appropriate small molecule TRIB ligands will further accelerate the transition of TRIB pseudokinase analysis into the mainstream of cell signalling. © 2015 Authors; published by Portland Press Limited.

A Novel Functional Role of Collagen Glycosylation

PubMed Central

Jürgensen, Henrik J.; Madsen, Daniel H.; Ingvarsen, Signe; Melander, Maria C.; Gårdsvoll, Henrik; Patthy, Laszlo; Engelholm, Lars H.; Behrendt, Niels

2011-01-01

Collagens make up the most abundant component of interstitial extracellular matrices and basement membranes. Collagen remodeling is a crucial process in many normal physiological events and in several pathological conditions. Some collagen subtypes contain specific carbohydrate side chains, the function of which is poorly known. The endocytic collagen receptor urokinase plasminogen activator receptor-associated protein (uPARAP)/Endo180 plays an important role in matrix remodeling through its ability to internalize collagen for lysosomal degradation. uPARAP/Endo180 is a member of the mannose receptor protein family. These proteins all include a fibronectin type II domain and a series of C-type lectin-like domains, of which only a minor part possess carbohydrate recognition activity. At least two of the family members, uPARAP/Endo180 and the mannose receptor, interact with collagens. The molecular basis for this interaction is known to involve the fibronectin type II domain but nothing is known about the function of the lectin domains in this respect. In this study, we have investigated a possible role of the single active lectin domain of uPARAP/Endo180 in the interaction with collagens. By expressing truncated recombinant uPARAP/Endo180 proteins and analyzing their interaction with collagens with high and low levels of glycosylation we demonstrated that this lectin domain interacts directly with glycosylated collagens. This interaction is functionally important because it was found to modulate the endocytic efficiency of the receptor toward highly glycosylated collagens such as basement membrane collagen IV. Surprisingly, this property was not shared by the mannose receptor, which internalized glycosylated collagens independently of its lectin function. This role of modulating its uptake efficiency by a specific receptor is a previously unrecognized function of collagen glycosylation. PMID:21768090

KSC-99pp1503

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- At SPACEHAB, in Titusville, Fla., STS-101 Mission Specialists Edward Tsang Lu (Ph.D.), at right, talks with workers about the SPACEHAB Logistics Double Module at left. The module is part of the payload for the mission. Lu and other crew members Commander James Donald Halsell Jr., Pilot Scott J. "Doc" Horowitz (Ph.D.), and Mission Specialists Mary Ellen Weber (Ph.D), Jeffrey N. Williams, and Boris W. Morukov and Yuri Malenchenko , who are with the Russian Space Agency , are taking part in a Crew Equipment Interface Test. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

Prebiotics and gut microbiota in chickens.

PubMed

Pourabedin, Mohsen; Zhao, Xin

2015-08-01

Prebiotics are non-digestible feed ingredients that are metabolized by specific members of intestinal microbiota and provide health benefits for the host. Fermentable oligosaccharides are best known prebiotics that have received increasing attention in poultry production. They act through diverse mechanisms, such as providing nutrients, preventing pathogen adhesion to host cells, interacting with host immune systems and affecting gut morphological structure, all presumably through modulation of intestinal microbiota. Currently, fructooligosaccharides, inulin and mannanoligosaccharides have shown promising results while other prebiotic candidates such as xylooligosaccharides are still at an early development stage. Despite a growing body of evidence reporting health benefits of prebiotics in chickens, very limited studies have been conducted to directly link health improvements to prebiotic-dependent changes in the gut microbiota. This article visits the current knowledge of the chicken gastrointestinal microbiota and reviews most recent publications related to the roles played by prebiotics in modulation of the gut microbiota and immune functions. Progress in this field will help us better understand how the gut microbiota contributes to poultry health and productivity, and support the development of new prebiotic products as an alternative to in-feed antibiotics. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Officials welcome the arrival of the Japanese Experiment Module

NASA Image and Video Library

2007-04-17

In the Space Station Processing Facility, NASA and Japanese Aerospace and Exploration Agency (JAXA) officials welcome the arrival of the Experiment Logistics Module Pressurized Section for the Japanese Experiment Module, or JEM, to the Kennedy Space Center. Seen here at right are JAXA representatives, including Japanese astronaut Takao Doi (center of front row), who is a crew member for mission STS-123 that will deliver the module to the space station. The new International Space Station component arrived at Kennedy March 12 to begin preparations for its future launch on mission STS-123. It will serve as an on-orbit storage area for materials, tools and supplies. It can hold up to eight experiment racks and will attach to the top of another larger pressurized module.

Kononenko reviews crew procedures

NASA Image and Video Library

2012-03-24

ISS030-E-171108 (24 March 2012) --- Russian cosmonaut Oleg Kononenko, Expedition 30 flight engineer, wearing a communication headset, is pictured in the Zvezda Service Module of the International Space Station as crew members prepare for their move to the appropriate Soyuz vehicles, due to the possibility that space debris could pass close to the station. Burbank, Shkaplerov and Ivanishin sheltered in the Soyuz TMA-22 spacecraft attached to the Poisk Mini-Research Module 2 (MRM2) while Kononenko, Kuipers and Pettit took to the Soyuz TMA-03M docked to the Rassvet Mini-Research Module 1 (MRM-1).

External Survey from Windows in Mini-Research Modules and Pirs Docking Compartment

NASA Image and Video Library

2013-04-03

ISS035-E-013901 (3 April 2013) --- This close-up picture of a Zvezda Service Module array, reflecting bright rays of the sun, thus creating an artistic scene, was photographed on April 3 by one of the Expedition 35 crew members as part of an External Survey from International Space Station windows that was recently added to the crew's task list.

A Comparison of Course Completion, Satisfaction, Achievement, and Performance among Non-Profit Professionals Who Complete Andragogical or Pedagogical Online Learning Modules on Grant Writing

ERIC Educational Resources Information Center

Bradley, Joe Bernard, Jr.

2010-01-01

The purpose of this study was to compare the outcomes among staff members of nonprofit social service agencies who participated in or completed an andragogically-facilitated or a pedagogically-conducted online learning module on foundation grant writing. The efficacy of andragogical methods is unknown and often debated due to scarce empirical…

Turbine seal assembly

DOEpatents

Little, David A.

2013-04-16

A seal assembly that limits gas leakage from a hot gas path to one or more disc cavities in a turbine engine. The seal assembly includes a seal apparatus that limits gas leakage from the hot gas path to a respective one of the disc cavities. The seal apparatus comprises a plurality of blade members rotatable with a blade structure. The blade members are associated with the blade structure and extend toward adjacent stationary components. Each blade member includes a leading edge and a trailing edge, the leading edge of each blade member being located circumferentially in front of the blade member's corresponding trailing edge in a direction of rotation of the turbine rotor. The blade members are arranged such that a space having a component in a circumferential direction is defined between adjacent circumferentially spaced blade members.

Wedge assembly for electrical transformer component spacing

DOEpatents

Baggett, Franklin E.; Cage, W. Franklin

1991-01-01

A wedge assembly that is easily inserted between two surfaces to be supported thereby, and thereafter expanded to produce a selected spacing between those surfaces. This wedge assembly has two outer members that are substantially identical except that they are mirror images of each other. Oppositely directed faces of these of these outer members are substantially parallel for the purpose of contacting the surfaces to be separated. The outer faces of these outer members that are directed toward each other are tapered so as to contact a center member having complementary tapers on both faces. A washer member is provided to contact a common end of the outer members, and a bolt member penetrates this washer and is threadably received in a receptor of the center member. As the bolt member is threaded into the center member, the center member is drawn further into the gap between the outer members and thereby separates these outer members to contact the surfaces to be separated. In the preferred embodiment, the contacting surfaces of the outer member and the center member are provided with guide elements. The wedge assembly is described for use in separating the secondary windings from the laminations of an electrical power transformer.

Networked Airborne Communications Using Adaptive Multi Beam Directional Links

DTIC Science & Technology

2016-03-05

Networked Airborne Communications Using Adaptive Multi-Beam Directional Links R. Bruce MacLeod Member, IEEE, and Adam Margetts Member, IEEE MIT...provide new techniques for increasing throughput in airborne adaptive directional net- works. By adaptive directional linking, we mean systems that can...techniques can dramatically increase the capacity in airborne networks. Advances in digital array technology are beginning to put these gains within reach

10-Gb/s direct modulation of polymer-based tunable external cavity lasers.

PubMed

Choi, Byung-Seok; Oh, Su Hwan; Kim, Ki Soo; Yoon, Ki-Hong; Kim, Hyun Soo; Park, Mi-Ran; Jeong, Jong Sool; Kwon, O-Kyun; Seo, Jun-Kyu; Lee, Hak-Kyu; Chung, Yun C

2012-08-27

We demonstrate a directly-modulated 10-Gb/s tunable external cavity laser (ECL) fabricated by using a polymer Bragg reflector and a high-speed superluminescent diode (SLD). The tuning range and output power of this ECL are measured to be >11 nm and 2.6 mW (@ 100 mA), respectively. We directly modulate this laser at 10 Gb/s and transmit the modulated signal over 20 km of standard single-mode fiber. The power penalty is measured to be <2.8 dB at the bit-error rate (BER) of 10(-10).

Timing of Survey Administration After Hospice Patient Death: Stability of Bereaved Respondents

PubMed Central

DiBiasio, Eleanor L.; Clark, Melissa A.; Gozalo, Pedro L.; Spence, Carol; Casarett, David J.; Teno, Joan M.

2017-01-01

Context The Centers for Medicare & Medicaid Services have elected to include a bereaved family member survey in public reporting of hospice quality data as mandated in the Affordable Care Act. However, it is not known what timepoint after death offers the most reliable responses. Objectives To examine the stability of bereaved family members’ survey responses when administered three, six and nine months after hospice patient death. Methods Bereaved family members from six geographically diverse hospices were interviewed three, six, and nine months after patient death. All respondents completed a core survey. Those whose family member died at home, in a free-standing inpatient unit, or in a nursing home also completed a site-specific module. Stability was based on top-box scoring of each item with kappa statistics, and multivariable regression models were used to assess directionality and predictors of change. To analyze the effects of grief, we assessed response stability among respondents at least one standard deviation from the mean change in grief between three and six months. Results We had 1532 surveys (536 three-month surveys, 529 six-month surveys, and 467 nine-month surveys) returned by 643 respondents (average age 61.7 years, 17.4% Black, 50.5% a child respondent) about hospice decedents (55.3% female, average age 78.6 years, 57.0% non-cancer, 40.0% at home.) The average kappa for core items between three and nine months was 0.54 (range: 0.42-0.74), 0.58 (0.41-0.69) for home-specific items, and 0.54 (0.39-0.63) for nursing home. Even among individuals demonstrating large grief changes, core items demonstrated moderate to high stability over time. Conclusion Bereaved family member responses are stable between three and nine months after the death of the patient. PMID:25647420

Direct Current Series Circuits: An Educational Module.

ERIC Educational Resources Information Center

Sturgess, Keith

This module was developed as remedial material for physics students who have difficulty understanding concepts of circuits and calculating resistances, and voltage drops and currents. Lists of prerequisite skills and instructional objectives are followed by a pretest (with answers). Students are directed to the subject matter in the module based…

Leaf expansion in Arabidopsis is controlled by a TCP-NGA regulatory module likely conserved in distantly related species.

PubMed

Ballester, Patricia; Navarrete-Gómez, Marisa; Carbonero, Pilar; Oñate-Sánchez, Luis; Ferrándiz, Cristina

2015-09-01

The NGATHA (NGA) clade of transcription factors (TFs) forms a small subfamily of four members in Arabidopsis thaliana. NGA genes act redundantly to direct the development of apical tissues in the gynoecium, where they have been shown to be essential for style and stigma specification. In addition, NGA genes have a more general role in controlling lateral organ growth. The four NGA genes in Arabidopsis are expressed in very similar domains, although little is known about the nature of their putative regulators. Here, we have identified a conserved region within the four NGA promoters that we have used as a bait to screen a yeast library, aiming to identify such NGA regulators. Three members of the TCP family of TFs, named after the founding factors TEOSINTE BRANCHED 1, CYCLOIDEA and PROLIFERATING CELL FACTOR 1 AND 2), were recovered from this screening, of which two [TCP2 and TCP3, members of the CINCINNATA (CIN) family of TCP genes (CIN-TCP) subclade] were shown to activate the NGA3 promoter in planta. We provide evidence that support that CIN-TCP genes are true regulators of NGA gene expression, and that part of the CIN-TCP role in leaf development is mediated by NGA upregulation. Moreover, we have found that this TCP-NGA regulatory interaction is likely conserved in angiosperms, including important crop species, for which the regulation of leaf development is a target for biotechnological improvement. © 2015 Scandinavian Plant Physiology Society.

Entering a New ERA: Education Resources and AGU

NASA Astrophysics Data System (ADS)

Karsten, J. L.; Johnson, R. M.

2001-12-01

Professional societies play a unique role in the on-going battle to improve public education in the Earth and space sciences. With guidance from its Committee on Education and Human Resources (CEHR), AGU has traditionally sponsored strong programs that provide mechanisms for linking its research membership with the formal/informal science education communities. Among the most successful of these are tutorials for K-12 teachers taught by AGU members during national meetings (e.g., GIFT - Geophysical Information For Teachers) and internships that allow teachers to experience geophysical science research first-hand (e.g., STaRS - Science Teacher and Research Scientist). AGU also co-sponsors major symposia to discuss and develop strategies for Earth science education reform (e.g., the NSF-sponsored Shaping the Future workshop) and provides an annual forum for the Heads and Chairs of undergraduate geoscience departments to discuss common problems and share solutions. In the fall of 2001, AGU expects to unveil a major new education and outreach website that will provide enhanced opportunities for communicating to students, teachers and the public about AGU members' research and new directions in geophysical science education. The most important contribution that AGU makes, however, is to validate and prominently endorse the education and outreach efforts of its members, both by sponsoring well-attended, education-related special sessions at AGU national meetings and by annually honoring individuals or groups with the Excellence in Geoscience Education award. Recent staff changes at AGU headquarters have brought new opportunities to expand upon these successful existing programs and move in other directions that capitalize on the strengths of the organization. Among new initiatives being considered are programs that partner education efforts with those being developed as part of several large research programs, curriculum modules that will promote teaching earth sciences-related materials within core physics, chemistry, and math curricula, and more sophisticated informal science education programs. Efforts to better coordinate AGU's education programs with those being developed by other professional geoscience organizations are also underway.

Developmental disabilities: improving competence in care using virtual patients.

PubMed

Sanders, Carla L; Kleinert, Harold L; Free, Teresa; King, Pam; Slusher, Ida; Boyd, Sara

2008-02-01

Nurse practitioners (NPs) have an increasingly important role in health care provision in the United States. However, most nurses report that they receive little or no clinical training in the area of developmental disabilities. A core development team consisting of NP faculty members from three universities, one physician assistant faculty member, the parents of children with developmental disabilities, and educational specialists developed two multimedia interactive pediatric instructional modules in CD-ROM format: one involving a child with Down syndrome and the other, an infant born at 26 weeks gestation. Participants were required to make decisions about proper clinical interaction throughout the cases. The modules on CD were piloted with NP students at three universities. Effectiveness study results demonstrated significant gains in both knowledge and comfort level regarding the care of patients with developmental disabilities.

Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells.

PubMed

Jarajapu, Yagna P R

2017-01-01

In recent years, previously unknown functions have been conferred to the RAAS and have been explored in mechanistic studies and disease models. Implication of bone marrow stem/progenitor cells in the cardiovascular protective or detrimental effects of RAAS is a prominent advancement because of the translational significance. Selected members of RAAS are now known to modulate migration, proliferation, and mobilization of bone marrow cells in response to ischemic insult, which are sensitive indicators of vascular repair-relevant functions. In this Chapter, protocols for most frequently used, in vitro, ex vivo, and in vivo assays to explore the potential of RAAS members to stimulate vascular repair-relevant functions of bone marrow stem/progenitor cells of human and murine origin.

WASP (Write a Scientific Paper): The right to privacy.

PubMed

Mallia, Pierre

2018-05-09

The data protection directive of the EU was set up to allow the freedom of movement of people between member states. This has the consequence of allowing the freedom of transfer of data between member states in research. The paper discusses three cases discussed during the project to see whether RECs can refuse research based on concern for data protection in some member states. Since the directive is not, as commonly thought, intended to protect the rights and freedoms of individuals - something which is the function of the Data Protection Acts of individual states, Member States do not have a right to prohibit research based on data laws. One must express concerns directly to Brussels. Conversely it is debated whether some research considered unlawful in one particular member state can be refused by this same state. Copyright © 2018. Published by Elsevier B.V.

Enhancement of the static extinction ratio by using a dual-section distributed feedback laser integrated with an electro-absorption modulator

NASA Astrophysics Data System (ADS)

Cho, Chun-Hyung; Kim, Jongseong; Sung, Hyuk-Kee

2016-09-01

We report on the enhancement of the static extinction ratio by using a dual-section distributed feedback laser diode integrated with an electro-absorption modulator. A directly- modulated dual-section laser can provide improved modulation performance under a low bias level ( i.e., below the threshold level) compared with a standard directly-modulated laser. By combining the extinction ratio from a dual-section laser with that from an electro-absorption modulator section, a total extinction ratio of 49.6. dB are successfully achieved.

Are self-directed work teams successful and effective tools for today`s organization?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arnwine, A.D.

1995-03-01

The purpose of this research is to (1) show the effectiveness and success of self-directed work teams within the organization, (2) emphasize the importance of team building in the success of the team, and (3) assist organizations in building self-directed work teams. The researcher used a direct survey and studied the following team building techniques: (1) Is the team`s mission clearly defined to each team member? (2) Are the goals clearly defined and achievable by all team members? (3) Will empowerment (decision-making power) be given equally to all team members? (4) Will open and honest communication be allowed among teammore » members? (5) Will each team member be respected and valued for his/her position on the team? (6) Are self-directed work teams effectively rewarded for accomplishments? (7) Have team members received adequate training to effectively complete their job tasks? Upon completion of the literature review and statistical data, and after analyzing the seven areas of team building techniques, it was determined three of the four teams were successful and effective. The only area of concern to the organization is that the participants felt they did not have true ownership of their teams; that is, team members were not given full empowerment. According to this study and the review of literature, full empowerment must be given to achieve successful and effective teams. If true empowerment is not given, the team will suffer in other areas of team building, and the organization will lose a valuable tool.« less

Direct fitness benefits and kinship of social foraging groups in an Old World tropical babbler

USGS Publications Warehouse

Kaiser, Sara A.; Martin, Thomas E.; Oteyza, Juan C.; Armstad, Connor E.; Fleischer, Robert C.

2018-01-01

Molecular studies have revealed that social groups composed mainly of nonrelatives may be widespread in group-living vertebrates, but the benefits favoring such sociality are not well understood. In the Old World, birds often form conspecific foraging groups that are maintained year-round and offspring usually disperse to other social groups. We tested the hypothesis that nonbreeding group members are largely unrelated and gain direct fitness benefits through breeding opportunities (males) and brood parasitism (females) in the tropical gray-throated babbler, Stachyris nigriceps, in Malaysian Borneo. Babblers foraged in social groups containing one or more breeding pairs (median = 8 group members of equal sex ratio), but group members rarely assisted with breeding (9% of 67 breeding pairs had a third helper; exhibiting facultative cooperative breeding). Although 20% of 266 group member dyads were first-order relatives of one or both members of the breeding pairs, 80% were unrelated. Male group members gained direct fitness benefits through extrapair and extra-group paternity (25% of 73 offspring), which was independent of their relatedness to the breeding pair and increased with decreasing group size. In contrast, females did not gain direct fitness benefits through brood parasitism. The low levels of relatedness and helping in social groups suggest that most group members do not gain indirect fitness benefits by helping to raise unrelated offspring. These findings highlight the importance of examining benefits of sociality for unrelated individuals that largely do not help and broaden the direct fitness benefits of group foraging beyond assumed survival benefits.

Inspiring Climate Education Excellence (ICEE): Developing self-directed professional development modules for secondary science teachers

NASA Astrophysics Data System (ADS)

Buhr, S. M.; Lynds, S. E.; McCaffrey, M. S.; Morton, E.

2010-12-01

Inspiring Climate Education Excellence (ICEE) is a NASA-funded project to develop online course modules and self-directed learning resources aligned with the Essential Principles of Climate Science. Following a national needs assessment survey and a face to face workshop to pilot test topics, a suite of online modules is being developed suitable for self-directed learning by secondary science teachers. Modules are designed around concepts and topics in which teachers express the most interest and need for instruction. Module design also includes attention to effective teaching strategies, such as awareness of student misconceptions, strategies for forestalling controversy and advice from master teachers on implementation and curriculum development. The resources are being developed in partnership with GLOBE, and the National Science Digital Library (NSDL) and is informed by the work of the Climate Literacy and Energy Awareness Network (CLEAN) project. ICEE will help to meet the professional development needs of teachers, including those participating in the GLOBE Student Climate Research Campaign. Modules and self-directed learning resources will be developed and disseminated in partnership with the National Science Digital Library (NSDL). This presentation introduces the needs assessment and pilot workshop data upon which the modules are based, and describes the modules that are available and in development.

Constant field gradient planar coupled cavity structure

DOEpatents

Kang, Y.W.; Kustom, R.L.

1999-07-27

A cavity structure is disclosed having at least two opposing planar housing members spaced apart to accommodate the passage of a particle beam through the structure between the members. Each of the housing members have a plurality of serially aligned hollows defined therein, and also passages, formed in the members, which interconnect serially adjacent hollows to provide communication between the hollows. The opposing planar housing members are spaced and aligned such that the hollows in one member cooperate with corresponding hollows in the other member to form a plurality of resonant cavities aligned along the particle beam within the cavity structure. To facilitate the obtaining of a constant field gradient within the cavity structure, the passages are configured so as to be incrementally narrower in the direction of travel of the particle beam. In addition, the spacing distance between the opposing housing members is configured to be incrementally smaller in the direction of travel of the beam. 16 figs.

Constant field gradient planar coupled cavity structure

DOEpatents

Kang, Yoon W.; Kustom, Robert L.

1999-01-01

A cavity structure having at least two opposing planar housing members spaced apart to accommodate the passage of a particle beam through the structure between the members. Each of the housing members have a plurality of serially aligned hollows defined therein, and also passages, formed in the members, which interconnect serially adjacent hollows to provide communication between the hollows. The opposing planar housing members are spaced and aligned such that the hollows in one member cooperate with corresponding hollows in the other member to form a plurality of resonant cavities aligned along the particle beam within the cavity structure. To facilitate the obtaining of a constant field gradient within the cavity structure, the passages are configured so as to be incrementally narrower in the direction of travel of the particle beam. In addition, the spacing distance between the opposing housing members is configured to be incrementally smaller in the direction of travel of the beam.

The rice F-box protein KISS ME DEADLY2 functions as a negative regulator of cytokinin signalling.

PubMed

Kim, Hyo Jung; Kieber, Joseph J; Schaller, G Eric

2013-01-01

Cytokinins are plant hormones that play critical roles in growth and development. We recently determined that the transcriptional response to cytokinin of Arabidopsis is modulated by the KISS ME DEADLY (KMD) family of F-box proteins. Here we demonstrate a conserved function for a member of the rice KMD family. Ectopic overexpression of OsKMD2 in Arabidopsis results in decreased cytokinin sensitivity based on a hypocotyl growth response assay, the decrease in sensitivity correlating with a decrease in the levels of the transcriptional regulator AtARR12. Furthermore, OsKMD2 directly interacts with AtARR12 based on yeast two-hybrid and co-immunoprecipitation assays. These results indicate that both monocots and dicots employ a similar KMD-dependent mechanism to regulate the transcriptional response to cytokinin.

Direct cooled power electronics substrate

DOEpatents

Wiles, Randy H [Powell, TN; Wereszczak, Andrew A [Oak Ridge, TN; Ayers, Curtis W [Kingston, TN; Lowe, Kirk T [Knoxville, TN

2010-09-14

The disclosure describes directly cooling a three-dimensional, direct metallization (DM) layer in a power electronics device. To enable sufficient cooling, coolant flow channels are formed within the ceramic substrate. The direct metallization layer (typically copper) may be bonded to the ceramic substrate, and semiconductor chips (such as IGBT and diodes) may be soldered or sintered onto the direct metallization layer to form a power electronics module. Multiple modules may be attached to cooling headers that provide in-flow and out-flow of coolant through the channels in the ceramic substrate. The modules and cooling header assembly are preferably sized to fit inside the core of a toroidal shaped capacitor.

Wakata with Fresh Fruit in the Cupola

NASA Image and Video Library

2014-04-01

ISS039-E-008095 (1 April 2014) --- Expedition 39 Commander Koichi Wakata, representing the Japan Aerospace Exploration Agency (JAXA), seems very elated that three new members of the crew have brought up food and supplies, especially fresh fruit, as depicted in this photo -- among the first sets of imagery documented with all six Expedition 39 crew members onboard the International Space Station. Wakata is in the orbiting outpost's Cupola module.

Telephone Support During Overseas Deployment for Military Spouses

DTIC Science & Technology

2017-12-01

other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a...negotiating roles and relationships; changes during deployment; strategies to support the spouse and the service member; and cues to alert spouses when to...14 o Table 4. Decision Making When Service Member (SM) Home and Deployed ............. 15 • Spouse Deployed Contents – Elearning modules

Final Report: High Power Semiconductor Laser Sources,

DTIC Science & Technology

1989-01-01

Mittelstein, Yasuhiko Arakawa, ) Anders Larssonb) and Amnon Yariv California Institute of Technology, Pasadena, California 91 125~412 (Received 7 July...Electronics and Commu- nication Engineers of Japan. He is a member of the Institute of Electronics Yasuhiko Arakawa S󈨑-M󈨔) was born in Ai- and...Gain, Modulation Response, and Spectral Linewidth in AlGaAs Quantum Well Lasers YASUHIKO ARAKAWA. MEMBER, IEEE. AND AMNON YARIV. FELLOW. IEEE Abstract

STS-102 Crew Patch

NASA Image and Video Library

2001-04-24

STS102-S-001 (January 2001) --- The central image on the STS-102 crew patch depicts the International Space Station (ISS) in the build configuration that it will have at the time of the arrival and docking of Discovery during the STS-102 mission, the first crew exchange flight to the space station. The station is shown along the direction of the flight as will be seen by the shuttle crew during their final approach and docking, the so-called V-bar approach. The names of the shuttle crew members are depicted in gold around the top of the patch, and surnames of the Expedition crew members being exchanged are shown in the lower banner. The three ribbons swirling up to and around the station signify the rotation of these ISS crew members. The number two is for the Expedition Two crew who fly up to the station, and the number one is for the Expedition One crew who then return down to Earth. In conjunction with the face of the Lab module of the station, these Expedition numbers create the shuttle mission number 102. Shown mated below the ISS is the Italian-built Multi-Purpose Logistics Module, Leonardo, that will fly for the first time on this flight, and which will be attached to the station by the shuttle crew during the docked phase of the mission. The flags of the countries that are the major contributors to this effort, the United States, Russia, and Italy are also shown in the lower part of the patch. The build-sequence number of this flight in the overall station assembly sequence, 5A.1, is captured by the constellations in the background. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

The adaptor protein SAP directly associates with PECAM-1 and regulates PECAM-1-mediated-cell adhesion in T-like cell lines.

PubMed

Proust, Richard; Crouin, Catherine; Gandji, Leslie Yewakon; Bertoglio, Jacques; Gesbert, Franck

2014-04-01

SAP is a small cytosolic adaptor protein expressed in hematopoietic lineages whose main function is to regulate intracellular signaling pathways induced by the triggering of members of the SLAM receptor family. In this paper, we have identified the adhesion molecule PECAM-1 as a new partner for SAP in a conditional yeast two-hybrid screen. PECAM-1 is an immunoglobulin-like molecule expressed by endothelial cells and leukocytes, which possesses both pro- and anti-inflammatory properties. However, little is known about PECAM-1 functions in T cells. We show that SAP directly and specifically interacts with the cytosolic tyrosine 686 of PECAM-1. We generated different T-like cell lines in which SAP or PECAM-1 are expressed or down modulated and we demonstrate that a diminished SAP expression correlates with a diminished PECAM-1-mediated adhesion. Although SAP has mainly been shown to associate with SLAM receptors, we evidence here that SAP is a new actor downstream of PECAM-1. Copyright © 2013 Elsevier Ltd. All rights reserved.

Direct electrical-to-optical conversion and light modulation in micro whispering-gallery-mode resonators

NASA Technical Reports Server (NTRS)

Maleki, Lute (Inventor); Levi, Anthony F. J. (Inventor)

2005-01-01

Techniques for directly converting an electrical signal into an optical signal by using a whispering gallery mode optical resonator formed of a dielectric material that allows for direct modulation of optical absorption by the electrical signal.

Locus of control, sources of motivation, and mental boundaries as antecedents of leader-member exchange quality.

PubMed

Barbuto, John E; Finch, Weltmer Dayna; Pennisi, Lisa A

2010-02-01

In this study were examined leaders' and members' scores on locus of control, sources of motivation, and mental boundaries to predict the quality of leader-member exchanges. 80 elected officials and their 388 direct reports were sampled in a field study. Analysis indicated followers' scores on locus of control, leaders' scores of self-concept internal motivation, leaders' scores on locus of control, and followers' rated goal-internalization motivation were positively related to leader-member exchanges. Implications and directions for research are discussed.

Timing of Survey Administration After Hospice Patient Death: Stability of Bereaved Respondents.

PubMed

DiBiasio, Eleanor L; Clark, Melissa A; Gozalo, Pedro L; Spence, Carol; Casarett, David J; Teno, Joan M

2015-07-01

The Centers for Medicare & Medicaid Services have elected to include a bereaved family member survey in public reporting of hospice quality data as mandated in the Affordable Care Act. However, it is not known what time point after death offers the most reliable responses. To examine the stability of bereaved family members' survey responses when administered three, six, and nine months after hospice patient death. Bereaved family members from six geographically diverse hospices were interviewed three, six, and nine months after patient death. All respondents completed a core survey. Those whose family member died at home, in a freestanding inpatient unit, or in a nursing home also completed a site-specific module. Stability was based on top-box scoring of each item with kappa statistics, and multivariable regression models were used to assess directionality and predictors of change. To analyze the effects of grief, we assessed response stability among respondents at least one SD from the mean change in grief between three and six months. We had 1532 surveys (536 three-month surveys, 529 six-month surveys, and 467 nine-month surveys) returned by 643 respondents (average age 61.7 years, 17.4% black, and 50.5% a child respondent) about hospice decedents (55.3% females, average age 78.6 years, 57.0% noncancer, and 40.0% at home). The average kappa for core items between three and nine months was 0.54 (range 0.42-0.74), 0.58 (0.41-0.69) for home-specific items, and 0.54 (0.39-0.63) for nursing home. Even among individuals demonstrating large grief changes, core items demonstrated moderate to high stability over time. Bereaved family member responses are stable between three and nine months after the death of the patient. Copyright © 2015 American Academy of Hospice and Palliative Medicine. Published by Elsevier Inc. All rights reserved.

Vegetable production facility as a part of a closed life support system in a Russian Martian space flight scenario

NASA Astrophysics Data System (ADS)

Berkovich, Yu. A.; Smolyanina, S. O.; Krivobok, N. M.; Erokhin, A. N.; Agureev, A. N.; Shanturin, N. A.

2009-07-01

A Manned Mars Mission scenario had been developed in frame of the Project 1172 supported International Science & Technology Center in Moscow. The Mars transit vehicle (MTV) supposed to have a crew of 4-6 with Pilot Laboratory compartment volume of 185 m 3 and with inner diameter of 4.1 m. A vegetable production facility with power consumption up to 10 kW is being considered as a component of the life support system to supply crew members by fresh vegetables during the mission. Proposed design of conveyor-type plant growth facility (PGF) comprised of 4-modules. Each module has a cylindrical planting surface and spiral cylindrical LED assembly to provide a high specific productivity relative to utilized onboard resources. Each module has a growth chamber that will be from 0.7 m to 1.5 m in length, and a crop illuminated area from 1.7 m 2 to 4.0 m 2. Leafy crops (cabbage, lettuce, spinach, chard, etc.) have been selected for module 1, primarily because of the highest specific productivity per consumed resources. Dietitians have recommended also carrot crop for module 2, pepper for module 3 and tomato for module 4. The maximal total PGF light energy estimated as 1.16 kW and total power consumption as about 7 kW. The module 1 characteristics have been calculated using own experimental data, information from the best on ground plant growth experiments with artificial light were used to predict crop productivity and biomass composition in the another modules. 4-module PGF could produce nearly 0.32 kg per crew member per day of fresh edible biomass, which would be about 50% of recommended daily vegetable supplement. An average crop harvest index is estimated as 0.75. The MTV food system could be entirely closed in terms of vitamins C and A with help of the PGF. In addition the system could provide 10-25% of essential minerals and vitamins of group B, and about 20% of food fibers. The present state of plant growth technology allows formulating of requirements specification for the flight-qualified modules.

77 FR 53950 - Self-Regulatory Organizations; EDGX Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-04

... data (collectively, ``DIRECT Logical Ports'').\\4\\ The Exchange proposes to reduce the quantity of free DIRECT Logical Ports from ten (10) sessions to five (5) sessions. Therefore, the Exchange will assess a monthly fee per logical port for Members and non-Members that maintain six or more DIRECT Logical Ports...

An Allosteric Coagonist Model for Propofol Effects on α1β2γ2L γ-Aminobutyric Acid Type A Receptors

PubMed Central

Ruesch, Dirk; Neumann, Elena; Wulf, Hinnerk; Forman, Stuart A.

2011-01-01

Background Propofol produces its major actions via γ-aminobutyric acid type A (GABAA) receptors. At low concentrations, propofol enhances agonist-stimulated GABAA receptor activity, and high propofol concentrations directly activate receptors. Etomidate produces similar effects, and there is convincing evidence that a single class of etomidate sites mediate both agonist modulation and direct GABAA receptor activation. It is unknown if the propofol binding site(s) on GABAA receptors that modulate agonist-induced activity also mediate direct activation. Methods GABAA α1β2γ2L receptors were heterologously expressed in Xenopus oocytes and activity was quantified using voltage clamp electrophysiology. We tested whether propofol and etomidate display the same linkage between agonist modulation and direct activation of GABAA receptors by identifying equi-efficacious drug solutions for direct activation. We then determined whether these drug solutions produce equal modulation of GABA-induced receptor activity. We also measured propofol-dependent direct activation and modulation of low GABA responses. Allosteric coagonist models similar to that established for etomidate, but with variable numbers of propofol sites, were fitted to combined data. Results Solutions of 19 μM propofol and 10 μM etomidate were found to equally activate GABAA receptors. These two drug solutions also produced indistinguishable modulation of GABA-induced receptor activity. Combined electrophysiological data behaved in a manner consistent with allosteric co-agonist models with more than one propofol site. The best fit was observed when the model assumed three equivalent propofol sites. Conclusions Our results support the hypothesis that propofol, like etomidate, acts at GABAA receptor sites mediating both GABA modulation and direct activation. PMID:22104494

Debris Impact Detection Instrument for Crewed Modules

NASA Technical Reports Server (NTRS)

Opiela, J.; Corsaro, R.; Giovanes, F.; Lio, J.-C.

2012-01-01

When micrometeoroid or debris impacts occur on a space habitat, crew members need to be quickly informed of the likely extent of damage, and be directed to the impact location for possible repairs. This is especially important because the outer walls of pressurized volumes are often not easily accessible, blocked by racks or cabinets. The goal of the Habitat Particle Impact Monitoring System (HIMS) is to develop a fully automated, end-to-end particle impact detection system for crewed space exploration modules. The HIMS uses multiple passive, thin film piezo-polymer vibration sensors to detect impacts on a surface, and computer processing of the acoustical signals to characterize the impacts. Development and demonstration of the HIMS is proceeding in concert with NASA's Habitat Demonstration Unit (HDU) Project. The HDU Project is designed to develop and test various technologies, configurations, and operational concepts for exploration habitats. This paper describes the HIMS development, initial testing, and HDU integration efforts. Initial tests of the system on the HDU were conducted at NASA s 2010 and 2011 Desert Research and Technologies Studies (Desert-RATS or D-RATS). The HDU lab module, as seen from above, has an open circular floorplan divided into eight wedge-shaped Segments. The side wall of the module -- the surface used for this technology demonstration -- is a hard fiberglass composite covered with a layer of sprayed-on foam insulation. Four sensor locations were assigned near the corners of a rectangular pattern on the wall of one segment of the HDU lab module. The flat, self-adhesive sensors were applied to the module during its initial outfitting. To study the influence of the wall s construction (thickness and materials), three sets of four sensors were installed at different layer depths: on the interior of the module s wall, on the exterior of the same wall, and on the exterior of the foam insulation. The signal produced when a vibration passes through a sensor is first sent through a pre-amplifier. The amplified signal then is sent to the data acquisition and data processing systems. The vibration data from the sensors are then processed and reduced to a form suitable for presentation to the crew.

The Roles of Orbital Frontal Cortex in the Modulation of Antisocial Behavior

ERIC Educational Resources Information Center

Blair, R. J. R.

2004-01-01

This article considers potential roles of orbital frontal cortex in the modulation of antisocial behavior. Two forms of aggression are distinguished: reactive aggression elicited in response to frustration/threat and goal directed, instrumental aggression. It is suggested that orbital frontal cortex is directly involved in the modulation of…

Modular bioreactor for the remediation of liquid streams and methods for using the same

DOEpatents

Noah, Karl S.; Sayer, Raymond L.; Thompson, David N.

1998-01-01

The present invention is directed to a bioreactor system for the remediation of contaminated liquid streams. The bioreactor system is composed of at least one and often a series of sub-units referred to as bioreactor modules. The modular nature of the system allows bioreactor systems be subdivided into smaller units and transported to waste sites where they are combined to form bioreactor systems of any size. The bioreactor modules further comprises reactor fill materials in the bioreactor module that remove the contaminants from the contaminated stream. To ensure that the stream thoroughly contacts the reactor fill materials, each bioreactor module comprises means for directing the flow of the stream in a vertical direction and means for directing the flow of the stream in a horizontal direction. In a preferred embodiment, the reactor fill comprises a sulfate reducing bacteria which is particularly useful for precipitating metals from acid mine streams.

Modular bioreactor for the remediation of liquid streams and methods for using the same

DOEpatents

Noah, K.S.; Sayer, R.L.; Thompson, D.N.

1998-06-30

The present invention is directed to a bioreactor system for the remediation of contaminated liquid streams. The bioreactor system is composed of at least one and often a series of sub-units referred to as bioreactor modules. The modular nature of the system allows bioreactor systems be subdivided into smaller units and transported to waste sites where they are combined to form bioreactor systems of any size. The bioreactor modules further comprises reactor fill materials in the bioreactor module that remove the contaminants from the contaminated stream. To ensure that the stream thoroughly contacts the reactor fill materials, each bioreactor module comprises means for directing the flow of the stream in a vertical direction and means for directing the flow of the stream in a horizontal direction. In a preferred embodiment, the reactor fill comprises a sulfate reducing bacteria which is particularly useful for precipitating metals from acid mine streams. 6 figs.

Maintenance of certification: current attitudes of members of the American Society of Neuroradiology.

PubMed

Yousem, D M

2008-02-01

The maintenance of certification (MOC) process has begun in radiology, and many physicians will be affected by it. We sought to assess the compliance with and knowledge of the MOC steps by American Society of Neuroradiology (ASNR) members. An on-line survey was distributed via e-mail to the members of the ASNR for whom e-mail addresses were available. The survey addressed 3 components of the MOC currently implemented: 1) MOC cognitive examinations, 2) self-assessment modules (SAMs), and 3) continuing medical education (CME) credits. The response rate was 1020/2662 (38.3%). Of those responding, 11% stated that they either do not support the MOC examination (8%) or were unaware of it (3%), 21.4% of respondents have completed an SAM module, but >30% were unaware that they had to complete SAMs or did not know where they were offered. Many members will receive most of their 25 required CME hours for 2007 from sources besides the ASNR annual meeting. Of the small proportion of respondents who made comments (247/1020, 24.2%), those about the MOC process were favorable in 6.1% (15/247), unfavorable in 46.6% (115/247), and neutral in 47.4% (117/247). The MOC process remains poorly understood, with limited compliance. The radiology societies should spend additional effort publicizing the requirements and offering support and products aimed at allowing their members to achieve compliance in a reasonable cost-effective fashion.

KSC-98pc1797

NASA Image and Video Library

1998-12-04

STS-88 Pilot Frederick W. "Rick" Sturckow is assisted with his ascent and re-entry flight suit in the white room at Launch Pad 39A before entering Space Shuttle Endeavour for launch. During the nearly 12-day mission, the six-member crew will mate the first two elements of the International Space Station the already-orbiting Zarya control module with the Unity connecting module carried by Endeavour. He is making his first spaceflight

View of the STS-88 crew in the Node 1/Unity module

NASA Image and Video Library

1998-12-10

STS088-322-035 (4 - 15 DECEMBER 1998) --- Three STS-88 crew members are pictured in one of two Pressurized Mating Adapters (PMA) connected to the Unity and Zarya modules. Taking pictures in the foreground is astronaut Jerry L. Ross, mission specialist. Others are astronaut Robert D. Cabana (left), mission commander, and cosmonaut Sergei K. Krikalev, mission specialist representing the Russian Space Agency (RSA).

Perceptions of D.M.D. student readiness for basic science courses in the United States: can online review modules help?

PubMed

Miller, C J; Aiken, S A; Metz, M J

2015-02-01

There can be a disconnect between the level of content covered in undergraduate coursework and the expectations of professional-level faculty of their incoming students. Some basic science faculty members may assume that students have a good knowledge base in the material and neglect to appropriately review, whilst others may spend too much class time reviewing basic material. It was hypothesised that the replacement of introductory didactic physiology lectures with interactive online modules could improve student preparedness prior to lectures. These modules would also allow faculty members to analyse incoming student abilities and save valuable face-to-face class time for alternative teaching strategies. Results indicated that the performance levels of incoming U.S. students were poor (57% average on a pre-test), and students often under-predicted their abilities (by 13% on average). Faculty expectations varied greatly between the different content areas and did not appear to correlate with the actual student performance. Three review modules were created which produced a statistically significant increase in post-test scores (46% increase, P < 0.0001, n = 114-115). The positive results of this study suggest a need to incorporate online review units in the basic science dental school courses and revise introductory material tailored to students' strengths and needs.

V-band integrated quadriphase modulator

NASA Technical Reports Server (NTRS)

Grote, A.; Chang, K.

1983-01-01

A V-band integrated circuit quadriphase shift keyed modulator/exciter for space communications systems was developed. Intersatellite communications systems require direct modulation at 60 GHz to enhance signal processing capability. For most systems, particularly space applications, small and lightweight components are essential to alleviate severe system design constraints. Thus to achieve wideband, high data rate systems, direct modulation techniques at millimeter waves using solid state integrated circuit technology are an integral part of the overall technology developments.

Web-based training course for evaluating radiological dose assessment in NRC's license termination process.

PubMed

Lepoire, D; Richmond, P; Cheng, J-J; Kamboj, S; Arnish, J; Chen, S Y; Barr, C; McKenney, C

2008-08-01

As part of the requirement for terminating the licenses of nuclear power plants or other nuclear facilities, license termination plans or decommissioning plans are submitted by the licensee to the U.S. Nuclear Regulatory Commission (NRC) for review and approval. Decommissioning plans generally refer to the decommissioning of nonreactor facilities, while license termination plans specifically refer to the decommissioning of nuclear reactor facilities. To provide a uniform and consistent review of dose modeling aspects of these plans and to address NRC-wide knowledge management issues, the NRC, in 2006, commissioned Argonne National Laboratory to develop a Web-based training course on reviewing radiological dose assessments for license termination. The course, which had first been developed in 2005 to target specific aspects of the review processes for license termination plans and decommissioning plans, evolved from a live classroom course into a Web-based training course in 2006. The objective of the Web-based training course is to train NRC staff members (who have various relevant job functions and are located at headquarters, regional offices, and site locations) to conduct an effective review of dose modeling in accordance with the latest NRC guidance, including NUREG-1757, Volumes 1 and 2. The exact size of the staff population who will receive the training has not yet been accurately determined but will depend on various factors such as the decommissioning activities at the NRC. This Web-based training course is designed to give NRC staff members modern, flexible access to training. To this end, the course is divided into 16 modules: 9 core modules that deal with basic topics, and 7 advanced modules that deal with complex issues or job-specific topics. The core and advanced modules are tailored to various NRC staff members with different job functions. The Web-based system uses the commercially available software Articulate, which incorporates audio, video, and animation in slide presentations and has glossary, document search, and Internet connectivity features. The training course has been implemented on an NRC system that allows staff members to register, select courses, track records, and self-administer quizzes.

Direct Gaze Modulates Face Recognition in Young Infants

ERIC Educational Resources Information Center

Farroni, Teresa; Massaccesi, Stefano; Menon, Enrica; Johnson, Mark H.

2007-01-01

From birth, infants prefer to look at faces that engage them in direct eye contact. In adults, direct gaze is known to modulate the processing of faces, including the recognition of individuals. In the present study, we investigate whether direction of gaze has any effect on face recognition in four-month-old infants. Four-month infants were shown…

KSC-07pd0893

NASA Image and Video Library

2007-04-17

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility, astronaut Takao Doi (left) and Commander Dominic Gorie pose in front of the Experiment Logistics Module Pressurized Section for the Japanese Experiment Module, or JEM, that recently arrived at Kennedy. Doi and Gorie are crew members for mission STS-123 that will deliver the logistics module to the International Space Station. Earlier, NASA and Japanese Aerospace and Exploration Agency (JAXA) officials welcomed the arrival of the module. The new International Space Station component arrived at Kennedy March 12 to begin preparations for its future launch on mission STS-123. It will serve as an on-orbit storage area for materials, tools and supplies. It can hold up to eight experiment racks and will attach to the top of another larger pressurized module. Photo credit: NASA/George Shelton

KSC-07pd0894

NASA Image and Video Library

2007-04-17

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility, astronaut Takao Doi (left) and Commander Dominic Gorie pose in front of the Experiment Logistics Module Pressurized Section for the Japanese Experiment Module, or JEM, that recently arrived at Kennedy. Doi and Gorie are crew members for mission STS-123 that will deliver the logistics module to the International Space Station. Earlier, NASA and Japanese Aerospace and Exploration Agency (JAXA) officials welcomed the arrival of the module. The new International Space Station component arrived at Kennedy March 12 to begin preparations for its future launch on mission STS-123. It will serve as an on-orbit storage area for materials, tools and supplies. It can hold up to eight experiment racks and will attach to the top of another larger pressurized module. Photo credit: NASA/George Shelton

KSC-2009-6508

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Michael Suffredini, program manager, International Space Station, NASA, addresses the invited guests at a ceremony transferring the ownership of node 3 for the International Space Station, looming in the background, from the European Space Agency, or ESA, to NASA. Seated, from left, are Bob Cabana, Kennedy Space Center director; Bernardo Patti, head of International Space Station, Program Department, ESA; and Secondino Brondolo, head of the Space Infrastructure, Thales Alenia Space Italy. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

KSC-2009-6507

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Michael Suffredini, program manager, International Space Station, NASA, addresses the invited guests at a ceremony transferring the ownership of node 3 for the International Space Station, looming in the background, from the European Space Agency, or ESA, to NASA. Seated, from left, are Michael Suffredini, program manager, International Space Station, NASA; William Dowdell, deputy for Operations, International Space Station and Spacecraft Processing, Kennedy; and Bernardo Patti, head of International Space Station, Program Department, ESA. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

KSC-2009-6505

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Kennedy Director Bob Cabana addresses the invited guests at a ceremony transferring the ownership of node 3 for the International Space Station, looming in the background, from the European Space Agency, or ESA, to NASA. Seated, from left, are William Dowdell, deputy for Operations, International Space Station and Spacecraft Processing, Kennedy; Bernardo Patti, head of International Space Station, Program Department, ESA; and Secondino Brondolo, head of the Space Infrastructure, Thales Alenia Space Italy. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

KSC-2009-6509

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Bernardo Patti, head of International Space Station, Program Department, ESA, addresses the invited guests at a ceremony transferring the ownership of node 3 for the International Space Station, looming in the background, from the European Space Agency, or ESA, to NASA. Seated, from left, are Bob Cabana, Kennedy Space Center director, and Secondino Brondolo, head of the Space Infrastructure, Thales Alenia Space Italy. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

Intestinal microbiota modulates gluten-induced immunopathology in humanized mice.

PubMed

Galipeau, Heather J; McCarville, Justin L; Huebener, Sina; Litwin, Owen; Meisel, Marlies; Jabri, Bana; Sanz, Yolanda; Murray, Joseph A; Jordana, Manel; Alaedini, Armin; Chirdo, Fernando G; Verdu, Elena F

2015-11-01

Celiac disease (CD) is an immune-mediated enteropathy triggered by gluten in genetically susceptible individuals. The recent increase in CD incidence suggests that additional environmental factors, such as intestinal microbiota alterations, are involved in its pathogenesis. However, there is no direct evidence of modulation of gluten-induced immunopathology by the microbiota. We investigated whether specific microbiota compositions influence immune responses to gluten in mice expressing the human DQ8 gene, which confers moderate CD genetic susceptibility. Germ-free mice, clean specific-pathogen-free (SPF) mice colonized with a microbiota devoid of opportunistic pathogens and Proteobacteria, and conventional SPF mice that harbor a complex microbiota that includes opportunistic pathogens were used. Clean SPF mice had attenuated responses to gluten compared to germ-free and conventional SPF mice. Germ-free mice developed increased intraepithelial lymphocytes, markers of intraepithelial lymphocyte cytotoxicity, gliadin-specific antibodies, and a proinflammatory gliadin-specific T-cell response. Antibiotic treatment, leading to Proteobacteria expansion, further enhanced gluten-induced immunopathology in conventional SPF mice. Protection against gluten-induced immunopathology in clean SPF mice was reversed after supplementation with a member of the Proteobacteria phylum, an enteroadherent Escherichia coli isolated from a CD patient. The intestinal microbiota can both positively and negatively modulate gluten-induced immunopathology in mice. In subjects with moderate genetic susceptibility, intestinal microbiota changes may be a factor that increases CD risk. Copyright © 2015 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

Intergroup relationships do not reduce racial bias in empathic neural responses to pain.

PubMed

Contreras-Huerta, Luis Sebastian; Hielscher, Emily; Sherwell, Chase S; Rens, Natalie; Cunnington, Ross

2014-11-01

Perceiving the pain of others activates similar neural structures to those involved in the direct experience of pain, including sensory and affective-motivational areas. Empathic responses can be modulated by race, such that stronger neural activation is elicited by the perception of pain in people of the same race compared with another race. In the present study, we aimed to identify when racial bias occurs in the time course of neural empathic responses to pain. We also investigated whether group affiliation could modulate the race effect. Using the minimal group paradigm, we assigned participants to one of two mixed-race teams. We examined event-related potentials from participants when viewing members of their own and the other team receiving painful or non-painful touch. We identified a significant racial bias in early ERP components at N1 over frontal electrodes, where Painful stimuli elicited a greater negative shift relative to Non-Painful stimuli in response to own race faces only. A long latency empathic response was also found at P3, where there was significant differentiation between Painful and Non-Painful stimuli regardless of Race or Group. There was no evidence that empathy-related brain activity was modulated by minimal group manipulation. These results support a model of empathy for pain that consists of early, automatic bias towards own-race empathic responses and a later top-down cognitive evaluation that does not differentiate between races and may ultimately lead to unbiased behaviour. Copyright © 2014 Elsevier Ltd. All rights reserved.

In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome

PubMed Central

Hernandez-Sanabria, Emma; Slomka, Vera; Herrero, Esteban R.; Kerckhof, Frederiek-Maarten; Zaidel, Lynette; Teughels, Wim; Boon, Nico

2017-01-01

Understanding the driving forces behind the shifts in the ecological balance of the oral microbiota will become essential for the future management and treatment of periodontitis. As the use of competitive approaches for modulating bacterial outgrowth is unexplored in the oral ecosystem, our study aimed to investigate both the associations among groups of functional compounds and the impact of individual substrates on selected members of the oral microbiome. We employed the Phenotype Microarray high-throughput technology to analyse the microbial cellular phenotypes of 15 oral bacteria. Multivariate statistical analysis was used to detect respiratory activity triggers and to assess similar metabolic activities. Carbon and nitrogen were relevant for the respiration of health-associated bacteria, explaining competitive interactions when grown in biofilms. Carbon, nitrogen, and peptides tended to decrease the respiratory activity of all pathobionts, but not significantly. None of the evaluated compounds significantly increased activity of pathobionts at both 24 and 48 h. Additionally, metabolite requirements of pathobionts were dissimilar, suggesting that collective modulation of their respiratory activity may be challenging. Flow cytometry indicated that the metabolic activity detected in the Biolog plates may not be a direct result of the number of bacterial cells. In addition, damage to the cell membrane may not influence overall respiratory activity. Our methodology confirmed previously reported competitive and collaborative interactions among bacterial groups, which could be used either as marker of health status or as targets for modulation of the oral environment. PMID:28638806

High-speed optical transmission system using 1.55-μm directly modulated lasers

NASA Astrophysics Data System (ADS)

Kim, Hoon

2018-01-01

We present the small-signal frequency responses of single-mode fiber used in directly modulated laser/direct detection (DML/DD) and externally modulated transmitter/direct detection (EXT/DD) systems, and compare the dispersion tolerance of these two systems. We find out that DML/DD system could be more tolerant to fiber chromatic dispersion than EXT/DD system when an electrical equalizer is employed at the receiver. We also present the transmission of 56- Gb/s 4-level pulse amplitude modulation signals generated from a 1.55-μm DML over 20-km standard single-mode fiber with the aid of a linear electrical equalizer. The performance behavior of this system with respect to the transmission distance is explained by using the frequency response.

Magnetoelastoelectric coupling in core-shell nanoparticles enabling directional and mode-selective magnetic control of THz beam propagation

DOE PAGES

Dutta, Moumita; Prasankumar, Rohit Prativadi; Natarajan, Kamaraju; ...

2017-08-07

Magnetoelastoelectric coupling in an engineered biphasic multiferroic nanocomposite enables a novel magnetic field direction-defined propagation control of terahertz (THz) waves. These core–shell nanoparticles are comprised of a ferromagnetic cobalt ferrite core and a ferroelectric barium titanate shell. Furthermore, an assembly of these nanoparticles, when operated in external magnetic fields, exhibits a controllable amplitude modulation when the magnetic field is applied antiparallel to the THz wave propagation direction; yet the same assembly displays an additional phase modulation when the magnetic field is applied along the propagation direction. And while field-induced magnetostriction of the core leads to amplitude modulation, phase modulation ismore » a result of stress-mediated piezoelectricity of the outer ferroelectric shell.« less

Sirtuins, Cell Senescence, and Vascular Aging.

PubMed

Kida, Yujiro; Goligorsky, Michael S

2016-05-01

The sirtuins (SIRTs) constitute a class of proteins with nicotinamide adenine dinucleotide-dependent deacetylase or adenosine diphosphate-ribosyltransferase activity. Seven SIRT family members have been identified in mammals, from SIRT1, the best studied for its role in vascular aging, to SIRT7. SIRT1 and SIRT2 are localized in the nucleus and cytoplasm. SIRT3, SIRT4, and SIRT5 are mitochondrial, and SIRT6 and SIRT7 are nuclear. Extensive studies have clearly revealed that SIRT proteins regulate diverse cell functions and responses to stressors. Vascular aging involves the aging process (senescence) of endothelial and vascular smooth muscle cells. Two types of cell senescence have been identified: (1) replicative senescence with telomere attrition; and (2) stress-induced premature senescence without telomere involvement. Both types of senescence induce vascular cell growth arrest and loss of vascular homeostasis, and contribute to the initiation and progression of cardiovascular diseases. Previous mechanistic studies have revealed in detail that SIRT1, SIRT3, and SIRT6 show protective functions against vascular aging, and definite vascular function of other SIRTs is under investigation. Thus, direct SIRT modulation and nicotinamide adenine dinucleotide stimulation of SIRT are promising candidates for cardiovascular disease therapy. A small number of pilot studies have been conducted to assess SIRT modulation in humans. These clinical studies have not yet provided convincing evidence that SIRT proteins alleviate morbidity and mortality in patients with cardiovascular diseases. The outcomes of multiple ongoing clinical trials are awaited to define the efficacy of SIRT modulators and SIRT activators in cardiovascular diseases, along with the potential adverse effects of chronic SIRT modulation. Copyright © 2016 Canadian Cardiovascular Society. Published by Elsevier Inc. All rights reserved.

Human Factors in Space Flight

NASA Technical Reports Server (NTRS)

Woolford, Barbara J.; Mount, Frances

2005-01-01

After forty years of experience with human space flight (Table 1), the current emphasis is on the design of space vehicles, habitats, and missions to ensure mission success. What lessons have we learned that will affect the design of spacecraft for future space exploration, leading up to exploring Mars? This chapter addresses this issue in four sections: Anthropometry and Biomechanics; Environmental Factors; Habitability and Architecture; and Crew Personal Sustenance. This introductory section introduces factors unique to space flight. A unique consideration for design of a habitable volume in a space vehicle is the lack of gravity during a space flight, referred to as microgravity. This affects all aspects of life, and drives special features in the habitat, equipment, tools, and procedures. The difference in gravity during a space mission requires designing for posture and motion differences. In Earth s gravity, or even with partial gravity, orientation is not a variable because the direction in which gravity acts defines up and down. In a microgravity environment the working position is arbitrary; there is no gravity cue. Orientation is defined primarily through visual cues. The orientation within a particular crew station or work area is referred to as local vertical, and should be consistent within a module to increase crew productivity. Equipment was intentionally arranged in various orientations in one module on Skylab to assess the efficiency in use of space versus the effects of inconsistent layout. The effects of that arrangement were confusion on entering the module, time spent in re-orientation, and conflicts in crew space requirements when multiple crew members were in the module. Design of a space vehicle is constrained by the three major mission drivers: mass, volume and power. Each of these factors drives the cost of a mission. Mass and volume determine the size of the launch vehicle directly; they can limit consumables such as air, water, and propellant; and they impact crew size and the types of activities the crew performs. Power is a limiting factor for a space vehicle. All environmental features (e.g., atmosphere, temperature, lighting) require power to maintain them. Power can be generated from batteries, from fuel cells, or from solar panels. Each of these sources requires lifting mass and volume from Earth, driving mission cost. All engineering decisions directly impact the design for habitation design and usage. For instance, if fuel cells are used they produce water, which is used for drinking and food preparation. If a different power source is used water has to be carried and stored on the vehicle which then directly impacts the food system choice as well as the launch weight of the vehicle.

Mechanisms of action of ligands of potential-dependent sodium channels.

PubMed

Tikhonov, D B

2008-06-01

Potential-dependent sodium channels play a leading role in generating action potentials in excitable cells. Sodium channels are the site of action of a variety of modulator ligands. Despite numerous studies, the mechanisms of action of many modulators remain incompletely understood. The main reason that many important questions cannot be resolved is that there is a lack of precise data on the structures of the channels themselves. Structurally, potential-dependent sodium channels are members of the P-loop channel superfamily, which also include potassium and calcium channels and glutamate receptor channels. Crystallization of a series of potassium channels showed that it was possible to analyze the structures of different members of the superfamily using the "homologous modeling" method. The present study addresses model investigations of the actions of ligands of sodium channels, including tetrodotoxin and batrachotoxin, as well as local anesthetics. Comparison of experimental data on sodium channel ligands with x-ray analysis data allowed us to reach a new level of understanding of the mechanisms of channel modulation and to propose a series of experimentally verifiable hypotheses.

Implementation of thermoelectric module for cooling process of microscale experimental room

NASA Astrophysics Data System (ADS)

Gołebiowska, Justyna; Żelazna, Agnieszka; Zioło, Paweł

2017-08-01

Thermoelectric modules, also known as Peltier modules, are used for cooling small devices and also, according to literature, in refrigeration. They can be an alternative to conventional refrigeration systems based on the use of compressors chillers powered by AC power. Peltier modules are powered by direct current (DC), which allows to power them directly supply by photovoltaic modules. In this paper operation of thermoelectric module used for cooling experimental room of cubature 0.125 m3 is presented. The study involves investigation of temperatures achieved on the cold and hot sides of module and inside the experimental room depending on the values of module supplying current. These studies provide an introduction to the assessment of the influence of different methods of heat removal on the hot side of thermoelectric module on cooling efficiency of whole system.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

At SPACEHAB, in Titusville, Fla., STS-101 Mission Specialists Edward Tsang Lu (Ph.D.), at right, talks with workers about the SPACEHAB Logistics Double Module at left. The module is part of the payload for the mission. Lu and other crew members Commander James Donald Halsell Jr., Pilot Scott J. 'Doc' Horowitz (Ph.D.), and Mission Specialists Mary Ellen Weber (Ph.D), Jeffrey N. Williams, and Boris W. Morukov and Yuri Malenchenko , who are with the Russian Space Agency , are taking part in a Crew Equipment Interface Test. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

MicroRNA-424/503 cluster members regulate bovine granulosa cell proliferation and cell cycle progression by targeting SMAD7 gene through activin signalling pathway.

PubMed

Pande, Hari Om; Tesfaye, Dawit; Hoelker, Michael; Gebremedhn, Samuel; Held, Eva; Neuhoff, Christiane; Tholen, Ernst; Schellander, Karl; Wondim, Dessie Salilew

2018-05-01

The granulosa cells are indispensable for follicular development and its function is orchestrated by several genes, which in turn posttranscriptionally regulated by microRNAs (miRNA). In our previous study, the miRRNA-424/503 cluster was found to be highly abundant in bovine granulosa cells (bGCs) of preovulatory dominant follicle compared to subordinate counterpart at day 19 of the bovine estrous cycle. Other study also indicated the involvement of miR-424/503 cluster in tumour cell resistance to apoptosis suggesting this miRNA cluster may involve in cell survival. However, the role of miR-424/503 cluster in granulosa cell function remains elusive Therefore, this study aimed to investigate the role of miRNA-424/503 cluster in bGCs function using microRNA gain- and loss-of-function approaches. The role of miR-424/503 cluster members in granulosa cell function was investigated by overexpressing or inhibiting its activity in vitro cultured granulosa cells using miR-424/503 mimic or inhibitor, respectively. Luciferase reporter assay showed that SMAD7 and ACVR2A are the direct targets of the miRNA-424/503 cluster members. In line with this, overexpression of miRNA-424/503 cluster members using its mimic and inhibition of its activity by its inhibitor reduced and increased, respectively the expression of SMAD7 and ACVR2A. Furthermore, flow cytometric analysis indicated that overexpression of miRNA-424/503 cluster members enhanced bGCs proliferation by promoting G1- to S- phase cell cycle transition. Modulation of miRNA-424/503 cluster members tended to increase phosphorylation of SMAD2/3 in the Activin signalling pathway. Moreover, sequence specific knockdown of SMAD7, the target gene of miRNA-424/503 cluster members, using small interfering RNA also revealed similar phenotypic and molecular alterations observed when miRNA-424/503 cluster members were overexpressed. Similarly, to get more insight about the role of miRNA-424/503 cluster members in activin signalling pathway, granulosa cells were treated with activin A. Activin A treatment increased cell proliferation and downregulation of both miRNA-424/503 members and its target gene, indicated the presence of negative feedback loop between activin A and the expression of miRNA-424/503. This study suggests that the miRNA-424/503 cluster members are involved in regulating bovine granulosa cell proliferation and cell cycle progression. Further, miRNA-424/503 cluster members target the SMAD7 and ACVR2A genes which are involved in the activin signalling pathway.

A 4-channel coil array interconnection by analog direct modulation optical link for 1.5-T MRI.

PubMed

Yuan, Jing; Wei, Juan; Shen, Gary X

2008-10-01

Optical glass fiber shows great advantages over coaxial cables in terms of electromagnetic interference, thus, it should be considered a potential alternative for magnetic resonance imaging (MRI) receive coil interconnection, especially for a large number coil array at high field. In this paper, we propose a 4-channel analog direct modulation optical link for a 1.5-T MRI coil array interconnection. First, a general direct modulated optical link is compared to an external modulated optical link. And then the link performances of the proposed direct modulated optical link, including power gain, frequency response, and dynamic range, are analyzed and measured. Phantom and in vivo head images obtained using this optical link are demonstrated for comparison with those obtained by cable connections. The signal-to-noise (SNR) analysis shows that the optical link achieves 6%-8% SNR a improvement over coaxial cables by elimination of electrical interference between cables during MR signal transmission.

Continuous equal channel angular pressing

DOEpatents

Zhu, Yuntian T.; Lowe, Terry C.; Valiev, Ruslan Z.; Raab, Georgy J.

2006-12-26

An apparatus that continuously processes a metal workpiece without substantially altering its cross section includes a wheel member having an endless circumferential groove, and a stationary constraint die that surrounds the wheel member, covers most of the length of the groove, and forms a passageway with the groove. The passageway has a rectangular shaped cross section. An abutment member projects from the die into the groove and blocks one end of the passageway. The wheel member rotates relative to the die in the direction toward the abutment member. An output channel in the die adjacent the abutment member has substantially the same cross section as the passageway. A metal workpiece is fed through an input channel into the passageway and carried in the groove by frictional drag in the direction towards the abutment member, and is extruded through the output channel without any substantial change in cross section.

Direct digital RF synthesis and modulation for MSAT mobile applications

NASA Technical Reports Server (NTRS)

Crozier, Stewart; Datta, Ravi; Sydor, John

1993-01-01

A practical method of performing direct digital RF synthesis using the Hilbert transform single sideband (SSB) technique is described. It is also shown that amplitude and phase modulation can be achieved directly at L-band with frequency stability and spurii performance exceeding stringent MSAT system requirements.

STS-102 crew members check out Discovery's payload bay

NASA Technical Reports Server (NTRS)

2001-01-01

Members of the STS-102 crew check out Discovery's payload bay in the Orbiter Processing Facility bay 1. Dressed in green, they are Mission Specialist Paul W. Richards (left) and Pilot James W. Kelly. The crew is at KSC for Crew Equipment Interface Test activities. Above their heads on the left side are two of the experiments being carried on the flight. STS-102 is the 8th construction flight to the International Space Station and will carry the Multi-Purpose Logistics Module Leonardo. STS-102 is scheduled for launch March 1, 2001. On that flight, Leonardo will be filled with equipment and supplies to outfit the U.S. laboratory module Destiny. The mission will also be carrying the Expedition Two crew to the Space Station, replacing the Expedition One crew who will return on Shuttle Discovery.

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Soichi Noguchi, who is with the Japanese Aerospace and Exploration Agency, looks at the inside of the Japanese Experiment Module (JEM) in the Space Station Processing Facility. He and other crew members are at KSC becoming familiar with Shuttle and mission equipment. The mission is Logistics Flight 1, which is scheduled to deliver supplies and equipment plus the external stowage platform to the International Space Station.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - STS-114 Mission Specialist Soichi Noguchi, who is with the Japanese Aerospace and Exploration Agency, looks at the inside of the Japanese Experiment Module (JEM) in the Space Station Processing Facility. He and other crew members are at KSC becoming familiar with Shuttle and mission equipment. The mission is Logistics Flight 1, which is scheduled to deliver supplies and equipment plus the external stowage platform to the International Space Station.

KSC-01pp1118

NASA Image and Video Library

2001-06-11

KENNEDY SPACE CENTER, Fla. -- STS-107 Payload Specialist Ilan Ramon, of Israel, manipulates a piece of equipment in the Spacehab module. He and other crew members are taking part in Crew Equipment Interface Test (CEIT) activities at SPACEHAB, Cape Canaveral, Fla. As a research mission, STS-107 will carry the Spacehab Double Module in its first research flight into space and a broad collection of experiments ranging from material science to life science. The CEIT activities enable the crew to perform certain flight operations, operate experiments in a flight-like environment, evaluate stowage locations and obtain additional exposure to specific experiment operations. Other STS-107 crew members are Commander Rick Douglas Husband, Pilot William C. McCool; Payload Commander Michael P. Anderson; and Mission Specialists Kalpana Chawla, Laurel Blair Salton Clark and David M. Brown. STS-107 is scheduled for launch May 23, 2002

Younger and older adults' collaborative recall of shared and unshared emotional pictures.

PubMed

Barber, Sarah J; Castrellon, Jaime J; Opitz, Philipp; Mather, Mara

2017-07-01

Although a group of people working together recalls more items than any one individual, they recall fewer unique items than the same number of people working apart whose responses are combined. This is known as collaborative inhibition, and it is a robust effect that occurs for both younger and older adults. However, almost all previous studies documenting collaborative inhibition have used stimuli that were neutral in emotional valence, low in arousal, and studied by all group members. In the current experiments, we tested the impact of picture-stimuli valence, picture-stimuli arousal, and information distribution in modulating the magnitude of collaborative inhibition. We included both younger and older adults because there are age differences in how people remember emotional pictures that could modulate any effects of emotion on collaborative inhibition. Results revealed that when information was shared (i.e., studied by all group members), there were robust collaborative inhibition effects for both neutral and emotional stimuli for both younger and older adults. However, when information was unshared (i.e., studied by only a single group member), these effects were attenuated. Together, these results provide mixed support for the retrieval strategy disruption account of collaborative inhibition. Supporting the retrieval strategy disruption account, unshared study information was less susceptible to collaborative inhibition than shared study information. Contradicting the retrieval strategy disruption account, emotional valence and arousal did not modulate the magnitude of collaborative inhibition despite the fact that participants clustered the emotional, but not neutral, information together in memory.

Elimination of residual amplitude modulation in tunable diode laser wavelength modulation spectroscopy using an optical fiber delay line.

PubMed

Chakraborty, Arup Lal; Ruxton, Keith; Johnstone, Walter; Lengden, Michael; Duffin, Kevin

2009-06-08

A new fiber-optic technique to eliminate residual amplitude modulation in tunable diode laser wavelength modulation spectroscopy is presented. The modulated laser output is split to pass in parallel through the gas measurement cell and an optical fiber delay line, with the modulation frequency / delay chosen to introduce a relative phase shift of pi between them. The two signals are balanced using a variable attenuator and recombined through a fiber coupler. In the absence of gas, the direct laser intensity modulation cancels, thereby eliminating the high background. The presence of gas induces a concentration-dependent imbalance at the coupler's output from which the absolute absorption profile is directly recovered with high accuracy using 1f detection.

Combined analysis of the radar cross-section modulation due to the long ocean waves around 14° and 34° incidence: Implication for the hydrodynamic modulation

NASA Astrophysics Data System (ADS)

Hauser, DanièLe; Caudal, GéRard

1996-11-01

The analysis of synthetic aperture radar observations over the ocean to derive the directional spectra of the waves is based upon a complex transfer function which is the sum of three terms: tilt modulation, hydrodynamic modulation, and velocity bunching effect. Both the hydrodynamic and the velocity bunching terms are still poorly known. Here we focus on the hydrodynamic part of the transfer function, from an experimental point of view. In this paper a new method is proposed to estimate the hydrodynamic modulation. The approach consists in analyzing observations obtained with an airborne real-aperture radar (called RESSAC). This radar (C band, HH polarized, broad beam of 14° × 3°) was used during the SEMAPHORE experiment, in two different modes. From the first mode (incidence angles from 7° to 21°) the directional spectra of the long waves are deduced under the assumption that the hydrodynamic modulation can be neglected (small incidence angles) and validated against in situ measurements. From the second mode (incidence angle from 27° to 41°) the amplitude and phase of the hydrodynamic modulation are deduced by combining the measured signal modulation spectrum at a mean incidence angle of 34° and the directional wave spectrum obtained from the first mode. The results, obtained in four different wind-wave cases of the SEMAPHORE experiment, show that the modulus of the hydrodynamic modulation is larger than that of the tilt modulation. Furthermore, we find that the modulus of the hydrodynamic transfer function is several times larger (by a factor 2-12) than the theoretical value proposed in previous works and 1.5-2.5 larger than experimental values reported in recent papers. The phase of the hydrodynamic modulation is found to be close to zero for waves propagating at an angle from the wind direction and between -20° and -40° for waves propagating along the wind direction. This indicates a significant influence of the wind-wave angle on the phase of the hydrodynamic modulation, in agreement with experimental results reported in recent papers.

Retro-modulators and fast beam steering for free-space optical communications

NASA Astrophysics Data System (ADS)

Chan, Trevor Keith

Free-space optical (FSO) communications is a means of secure, high bandwidth communication through the use of a modulated laser beam in free-space as the information medium. The chaotic nature of the atmosphere and the motion of the communication nodes make laser alignment a crucial concern. The employment of retro-reflecting modulators makes the bidirectional quality of a communication link into a one sided alignment problem. While there are existing retro-reflecting modulators, their trade-offs create a lack of abilities (such as aperture size, angular range, high modulation speeds, economic viability) which do not fulfill the requirements for certain applications. Also, the beam must be directed towards the intended receiver. Form mobile or scintillated communication links, beam direction must be adaptable in real time. Once again, this area suffers from trade-offs where beamsteering speed is often limited. Research used to mitigate the trade-offs and adapt the devices into viable options for a wider range of applications is explored in this dissertation. Two forms of retro-modulators were explored; a MEMS deformable mirror retro-modulator and a solid silicon retro-modulator that modulated the light by frustrated total internal reflection (FTIR). The MEMS version offered a high speed, scalable, wavelength/angle insensitive retro-modulator which can be massed produced at low cost, while the solid retro-modulator offered a large field of view with low cost as well. Both modulator's design, simulated performances, fabrication and experimental characterization are described in this dissertation. An ultra-fast beamscanner was also designed using 2-dimensional dispersion. By using wavelength switching for directional control, a beamscanner was developed that could switch light faster than pre-existing beamscanners while the beams characteristics (most importantly its aperture) could be freely adjusted by the independent optics. This beamscanner was preceded by our work on a large channel wavelength demultiplexer which combined two orthogonally oriented wavelength demultiplexers. This created a 2-dimensional array of spots in free-space. The light was directed be a collimating lens into a specific direction based on its wavelength. The performance of this beamscanner was simulated by modeling the dispersive properties of the components.

Feature-Based Attention in Early Vision for the Modulation of Figure–Ground Segregation

PubMed Central

Wagatsuma, Nobuhiko; Oki, Megumi; Sakai, Ko

2013-01-01

We investigated psychophysically whether feature-based attention modulates the perception of figure–ground (F–G) segregation and, based on the results, we investigated computationally the neural mechanisms underlying attention modulation. In the psychophysical experiments, the attention of participants was drawn to a specific motion direction and they were then asked to judge the side of figure in an ambiguous figure with surfaces consisting of distinct motion directions. The results of these experiments showed that the surface consisting of the attended direction of motion was more frequently observed as figure, with a degree comparable to that of spatial attention (Wagatsuma et al., 2008). These experiments also showed that perception was dependent on the distribution of feature contrast, specifically the motion direction differences. These results led us to hypothesize that feature-based attention functions in a framework similar to that of spatial attention. We proposed a V1–V2 model in which feature-based attention modulates the contrast of low-level feature in V1, and this modulation of contrast changes directly the surround modulation of border-ownership-selective cells in V2; thus, perception of F–G is biased. The model exhibited good agreement with human perception in the magnitude of attention modulation and its invariance among stimuli. These results indicate that early-level features that are modified by feature-based attention alter subsequent processing along afferent pathway, and that such modification could even change the perception of object. PMID:23515841

Feature-based attention in early vision for the modulation of figure-ground segregation.

PubMed

Wagatsuma, Nobuhiko; Oki, Megumi; Sakai, Ko

2013-01-01

We investigated psychophysically whether feature-based attention modulates the perception of figure-ground (F-G) segregation and, based on the results, we investigated computationally the neural mechanisms underlying attention modulation. In the psychophysical experiments, the attention of participants was drawn to a specific motion direction and they were then asked to judge the side of figure in an ambiguous figure with surfaces consisting of distinct motion directions. The results of these experiments showed that the surface consisting of the attended direction of motion was more frequently observed as figure, with a degree comparable to that of spatial attention (Wagatsuma et al., 2008). These experiments also showed that perception was dependent on the distribution of feature contrast, specifically the motion direction differences. These results led us to hypothesize that feature-based attention functions in a framework similar to that of spatial attention. We proposed a V1-V2 model in which feature-based attention modulates the contrast of low-level feature in V1, and this modulation of contrast changes directly the surround modulation of border-ownership-selective cells in V2; thus, perception of F-G is biased. The model exhibited good agreement with human perception in the magnitude of attention modulation and its invariance among stimuli. These results indicate that early-level features that are modified by feature-based attention alter subsequent processing along afferent pathway, and that such modification could even change the perception of object.

Opposing effects of allogrooming on disease transmission in ant societies

PubMed Central

Theis, Fabian J.; Ugelvig, Line V.; Marr, Carsten; Cremer, Sylvia

2015-01-01

To prevent epidemics, insect societies have evolved collective disease defences that are highly effective at curing exposed individuals and limiting disease transmission to healthy group members. Grooming is an important sanitary behaviour—either performed towards oneself (self-grooming) or towards others (allogrooming)—to remove infectious agents from the body surface of exposed individuals, but at the risk of disease contraction by the groomer. We use garden ants (Lasius neglectus) and the fungal pathogen Metarhizium as a model system to study how pathogen presence affects self-grooming and allogrooming between exposed and healthy individuals. We develop an epidemiological SIS model to explore how experimentally observed grooming patterns affect disease spread within the colony, thereby providing a direct link between the expression and direction of sanitary behaviours, and their effects on colony-level epidemiology. We find that fungus-exposed ants increase self-grooming, while simultaneously decreasing allogrooming. This behavioural modulation seems universally adaptive and is predicted to contain disease spread in a great variety of host–pathogen systems. In contrast, allogrooming directed towards pathogen-exposed individuals might both increase and decrease disease risk. Our model reveals that the effect of allogrooming depends on the balance between pathogen infectiousness and efficiency of social host defences, which are likely to vary across host–pathogen systems. PMID:25870394

Food Lab - Technician - MSC

NASA Image and Video Library

1970-04-14

S70-34847 (11 April 1970) --- Astronaut John L. Swigert Jr., command module pilot for NASA?s third lunar landing mission, appears to be relaxing in the suiting room at Kennedy Space Center prior to launch. Other members of the Apollo 13 crew include astronauts James A. Lovell Jr., commander, and Fred W. Haise Jr., lunar module pilot. Swigert replaced astronaut Thomas K. Mattingly II when it was discovered that Mattingly had been exposed to the measles.

Lactobacilli Activate Human Dendritic Cells that Skew T Cells Toward T Helper 1 Polarization

DTIC Science & Technology

2005-01-06

Species Modulate the Phenotype and Function of MDCs. Previous studies have shown that Lactobacillus plantarum and Lactobacillus rhamnosus can induce...cell immune responses at both systemic and mucosal sites. Many Lactobacillus species are normal members of the human gut microflora and most are regarded...several well defined strains, representing three species of Lactobacillus on human myeloid DCs (MDCs) and found that they modulated the phenotype and

KSC-98pc1807

NASA Image and Video Library

1998-12-04

In a firing room of the Launch Control Center, U.S. Secretary of State Madeleine Albright speaks to the launch team after the successful launch of Space Shuttle Endeavour at 3:35:34 a.m. EST. During the nearly 12-day mission of STS-88, the six-member crew will mate in space the first two elements of the International Space Station the already-orbiting Zarya control module and the Unity connecting module carried by Endeavour

KSC-98pc1808

NASA Image and Video Library

1998-12-04

In a firing room of the Launch Control Center,U.S. Secretary of State Madeleine Albright waves to the personnel after her speech about the successful launch of Space Shuttle Endeavour. At her right is NASA Administrator Daniel Goldin. During the nearly 12-day mission of STS-88, the six-member crew will mate in space the first two elements of the International Space Station the already-orbiting Zarya control module and the Unity connecting module carried by Endeavour

Array of planar membrane modules for producing hydrogen

DOEpatents

Vencill, Thomas R [Albuquerque, NM; Chellappa, Anand S [Albuquerque, NM; Rathod, Shailendra B [Hillsboro, OR

2012-05-08

A shared or common environment membrane reactor containing a plurality of planar membrane modules with top and bottom thin foil membranes supported by both an intermediary porous support plate and a central base which has both solid extended members and hollow regions or a hollow region whereby the two sides of the base are in fluid communication. The membrane reactor operates at elevate temperatures for generating hydrogen from hydrogen rich feed fuels.

STS-135 crew during AEM (Animal Enclosure Module) training

NASA Image and Video Library

2011-03-25

JSC2011-E-029133 (25 March 2011) --- STS-135 crew members participate in an Animal Enclosure Module (AEM) training session in the Jake Garn Simulation and Training Facility at NASA's Johnson Space Center. Pictured from the right are NASA astronauts Chris Ferguson, commander; Sandy Magnus and Rex Walheim, both mission specialists. STS-135 is planned to be the final mission of the space shuttle program. Photo credit: NASA or National Aeronautics and Space Administration

Demonstration, Developmental and Research Project for Programs, Materials, Facilities and Educational Technology for Undereducated Adults: Ohio State Module. ABE Life-Centered Curriculum Development and Teaching Technique. Final Report.

ERIC Educational Resources Information Center

Morehead State Univ., KY.

This workshop was held for the purpose of training selected staff members of the Ohio Module Field Unit of the Appalachian Adult Basic Education Demonstration Center (AABEDC). Twelve persons, six teachers and six paraprofessionals, were selected to participate in the workshop. While their specific jobs vary, all will be concerned with utilization…

Convective stability in the Rayleigh-Benard and directional solidification problems - High-frequency gravity modulation

NASA Technical Reports Server (NTRS)

Wheeler, A. A.; Mcfadden, G. B.; Murray, B. T.; Coriell, S. R.

1991-01-01

The effect of vertical, sinusoidal, time-dependent gravitational acceleration on the onset of solutal convection during directional solidification is analyzed in the limit of large modulation frequency. When the unmodulated state is unstable, the modulation amplitude required to stabilize the system is determined by the method of averaging. When the unmodulated state is stable, resonant modes of instability occur at large modulation amplitude. These are analyzed using matched asymptotic expansions to elucidate the boundary-layer structure for both the Rayleigh-Benard and directional solidification configurations. Based on these analyses, a thorough examination of the dependence of the stability criteria on the unmodulated Rayleigh number, Schmidt number, and distribution coefficient, is carried out.

Reconfigurable WDM-PON empowered by a low-cost 8-channel directly modulated laser module

NASA Astrophysics Data System (ADS)

Zhang, Yi-ming; Liu, Yu; Zhang, Zhi-ke; Zhao, Ze-ping; Tian, Ye; Zhu, Ning-hua

2017-11-01

A 10 Gbit/s 16-km-long reconfigurable wavelength-division-multiplexing passive optical network (WDM-PON) is presented empowered by a low-cost multi-channel directly modulated laser (DML) module. Compared with the case using discrete devices in conventional scheme, the proposed DML module provides a cost-effective solution with reduced complexity. The clear eye diagram and the bit error rate ( BER) of less than 2×10-7 with a sensitivity of -7 dBm are obtained. Due to the special packaging design, the crosstalk between channels under condition of simultaneous operation can be negligible.

Three-axis force actuator for a magnetic bearing

NASA Technical Reports Server (NTRS)

Gondhalekar, Vijay (Inventor)

1998-01-01

This invention features a three-axis force actuator that axially, radially and rotatably supports a bearing member for frictionless rotation about an axis of rotation generally coincident with a Z-axis. Also featured is a magnetic bearing having such an actuator. The actuator includes an inner member, a magnetic member and a pole assembly having a ring member and four pole extending therefrom. The poles are equi-angular spaced from each other and radially spaced about the Z-axis. The inner member extends along the Z-axis and is a highly magnetic permeable material. The magnetic member is formed about the inner member outer surface, extends along the Z-axis and is configured so one magnetic pole polarity is located at its outer surface and the other polarity pole is located at its inner surface. Preferably, the magnetic member is a radially magnetized permanent magnet. The inner surface of the ring member is magnetically coupled to the magnetic member and a face of each pole is coupled to the bearing member. The magnetic member, the pole assembly, the inner member and the bearing member cooperate to generate a magnetic field that radially and rotatably supports a rotating member secured to the bearing member. The actuator further includes a plurality of electromagnetic coils. Preferably, a coil is formed about each pole and at least 2 coils are formed about the inner member. When energized, the electromagnetic coils generate a modulated magnetic field that stabilizes the rotating member in the desired operational position.

A new linear structured light module based on the MEMS micromirror

NASA Astrophysics Data System (ADS)

Zhou, Peng; Shen, Wenjiang; Yu, Huijun

2017-10-01

A new linear structured light module based on the Micro-Electro-Mechanical System (MEMS) two-dimensional scanning micromirror was designed and created. This module consists of a laser diode, a convex lens, and the MEMS micromirror. The laser diode generates the light and the convex lens control the laser beam to converge on a single point with large depth of focus. The fast scan in horizontal direction of the micromirror will turn the laser spot into a homogenous laser line. Meanwhile, the slow scan in vertical direction of the micromirror will move the laser line in the vertical direction. The width of the line generated by this module is 300μm and the length is 120mm and the moving distance is 100mm at 30cm away from the module. It will promote the development of industrial detection.

Post-fire assessment of structural wood members

Treesearch

Robert J. Ross; Brian K. Brashaw; Xiping Wang; Robert H. White; Roy F. Pellerin

2005-01-01

Since the interior of a charred wood member normally retains its structural integrity, large structural wood members often do not need to be replaced after a fire. Engineering judgement is required to determine which members can remain and which members need to be replaced or repaired. Due to the lack of established methods to directly determine the residual capacity...

Dynamic Network-Based Relevance Score Reveals Essential Proteins and Functional Modules in Directed Differentiation

PubMed Central

Wu, Chia-Chou; Lin, Che

2015-01-01

The induction of stem cells toward a desired differentiation direction is required for the advancement of stem cell-based therapies. Despite successful demonstrations of the control of differentiation direction, the effective use of stem cell-based therapies suffers from a lack of systematic knowledge regarding the mechanisms underlying directed differentiation. Using dynamic modeling and the temporal microarray data of three differentiation stages, three dynamic protein-protein interaction networks were constructed. The interaction difference networks derived from the constructed networks systematically delineated the evolution of interaction variations and the underlying mechanisms. A proposed relevance score identified the essential components in the directed differentiation. Inspection of well-known proteins and functional modules in the directed differentiation showed the plausibility of the proposed relevance score, with the higher scores of several proteins and function modules indicating their essential roles in the directed differentiation. During the differentiation process, the proteins and functional modules with higher relevance scores also became more specific to the neuronal identity. Ultimately, the essential components revealed by the relevance scores may play a role in controlling the direction of differentiation. In addition, these components may serve as a starting point for understanding the systematic mechanisms of directed differentiation and for increasing the efficiency of stem cell-based therapies. PMID:25977693

Microinverters for employment in connection with photovoltaic modules

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lentine, Anthony L.; Nielson, Gregory N.; Okandan, Murat

2015-09-22

Microinverters useable in association with photovoltaic modules are described. A three phase-microinverter receives direct current output generated by a microsystems-enabled photovoltaic cell and converts such direct current output into three-phase alternating current out. The three-phase microinverter is interleaved with other three-phase-microinverters, wherein such microinverters are integrated in a photovoltaic module with the microsystems-enabled photovoltaic cell.

Asymmetry of perceived key movement in chorale sequences: converging evidence from a probe-tone analysis.

PubMed

Cuddy, L L; Thompson, W F

1992-01-01

In a probe-tone experiment, two groups of listeners--one trained, the other untrained, in traditional music theory--rated the goodness of fit of each of the 12 notes of the chromatic scale to four-voice harmonic sequences. Sequences were 12 simplified excerpts from Bach chorales, 4 nonmodulating, and 8 modulating. Modulations occurred either one or two steps in either the clockwise or the counterclockwise direction on the cycle of fifths. A consistent pattern of probe-tone ratings was obtained for each sequence, with no significant differences between listener groups. Two methods of analysis (Fourier analysis and regression analysis) revealed a directional asymmetry in the perceived key movement conveyed by modulating sequences. For a given modulation distance, modulations in the counterclockwise direction effected a clearer shift in tonal organization toward the final key than did clockwise modulations. The nature of the directional asymmetry was consistent with results reported for identification and rating of key change in the sequences (Thompson & Cuddy, 1989a). Further, according to the multiple-regression analysis, probe-tone ratings did not merely reflect the distribution of tones in the sequence. Rather, ratings were sensitive to the temporal structure of the tonal organization in the sequence.

Structural support bracket for gas flow path

DOEpatents

None

2016-08-02

A structural support system is provided in a can annular gas turbine engine having an arrangement including a plurality of integrated exit pieces (IEPs) forming an annular chamber for delivering gases from a plurality of combustors to a first row of turbine blades. A bracket structure is connected between an IEP and an inner support structure on the engine. The bracket structure includes an axial bracket member attached to an IEP and extending axially in a forward direction. A transverse bracket member has an end attached to the inner support structure and extends circumferentially to a connection with a forward end of the axial bracket member. The transverse bracket member provides a fixed radial position for the forward end of the axial bracket member and is flexible in the axial direction to permit axial movement of the axial bracket member.

Design of selective nuclear receptor modulators: RAR and RXR as a case study.

PubMed

de Lera, Angel R; Bourguet, William; Altucci, Lucia; Gronemeyer, Hinrich

2007-10-01

Retinoic acid receptors (RARs) and retinoid X receptors (RXRs) are members of the nuclear receptor superfamily whose effects on cell growth and survival can be modulated therapeutically by small-molecule ligands. Although compounds that target these receptors are powerful anticancer drugs, their use is limited by toxicity. An improved understanding of the structural biology of RXRs and RARs and recent advances in the chemical synthesis of modified retinoid and rexinoid ligands should enable the rational design of more selective agents that might overcome such problems. Here, we review structural data for RXRs and RARs, discuss strategies in the design of selective RXR and RAR modulators, and consider lessons that can be learned for the design of selective nuclear-receptor modulators in general.

Ubiquitous Adoption of Innovative and Supportive Information and Communications Technology Across Health and Social Care Needs Education for Clinicians.

PubMed

Procter, Paula M

2017-01-01

The paper presents the development, use and evaluation of an on-line undergraduate module delivering an academic-led programme of eHealth learning within nursing, midwifery, allied health professional and social work courses. The health information technology competency frameworks are explored along with an overview of the resulting module. The need for an academically led module will be made along with a description of the management required to maintain validity of content materials. A review of student evaluations will be presented. In conclusion the positive change in attitude and understanding of academic staff members towards health information technology through the inclusion of the module across all of the undergraduate courses will be explored.

Apollo 10 view of the Earth

NASA Image and Video Library

1969-05-18

AS10-34-5026 (18-26 May 1969) --- An Apollo 10 photograph of Earth taken from 100,000 miles away. Visible are many areas of Europe and Africa. Among the features and countries identifiable are Portugal, Spain, Italy, the Mediterranean Sea, Greece, Turkey, Bulgaria, the Black Sea, Libya, Egypt, Saudi Arabia, the Sinai Peninsula, the Nile Delta, Lake Chad, and South Africa. The crew members for Apollo 10 are astronauts Thomas P. Stafford, commander; John W. Young, command module pilot; and Eugene E. Cernan, lunar module pilot. Astronaut Young remained in lunar orbit, in the Command and Service Modules (CSM) "Charlie Brown", while astronauts Stafford and Cernan descended to within nine miles of the lunar surface, in the Lunar Module (LM) "Snoopy".

Monthly modulation in dark matter direct-detection experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Britto, Vivian; Meyers, Joel, E-mail: vivian.britto@mail.utoronto.ca, E-mail: jmeyers@cita.utoronto.ca

2015-11-01

The signals in dark matter direct-detection experiments should exhibit modulation signatures due to the Earth's motion with respect to the Galactic dark matter halo. The annual and daily modulations, due to the Earth's revolution about the Sun and rotation about its own axis, have been explored previously. Monthly modulation is another such feature present in direct detection signals, and provides a nearly model-independent method of distinguishing dark matter signal events from background. We study here monthly modulations in detail for both WIMP and WISP dark matter searches, examining both the effect of the motion of the Earth about the Earth-Moonmore » barycenter and the gravitational focusing due to the Moon. For WIMP searches, we calculate the monthly modulation of the count rate and show the effects are too small to be observed in the foreseeable future. For WISP dark matter experiments, we show that the photons generated by WISP to photon conversion have frequencies which undergo a monthly modulating shift which is detectable with current technology and which cannot in general be neglected in high resolution WISP searches.« less

Expedition 32 Crew Members work in the SM

NASA Image and Video Library

2012-07-22

ISS032-E-010076 (22 July 2012) --- Russian cosmonaut Sergei Revin (left) and NASA astronaut Joe Acaba, both Expedition 32 flight engineers, use a computer in the Zvezda Service Module of the International Space Station.

N2pc is modulated by stimulus-stimulus, but not by stimulus-response incompatibilities.

PubMed

Cespón, J; Galdo-Álvarez, S; Díaz, F

2013-04-01

Studies of the N2pc in Simon-type tasks have revealed inconsistent results. That is, N2pc was only modulated when a stimulus-stimulus (S-S) overlap covaries with the stimulus-response (S-R) overlap. The present study aimed to establish whether N2pc is modulated by the S-R or by the S-S overlap. Therefore, we designed a Simon task requiring response to a colour stimulus (an arrow) with two irrelevant dimensions (position and direction). The following conditions were thus generated: compatible direction-compatible position (CDCP); incompatible direction-compatible position (IDCP); compatible direction-incompatible position (CDIP); and incompatible direction-incompatible position (IDIP). In IDCP and CDIP, both irrelevant dimensions conveyed contradictory spatial information (S-S incompatibility), while compatibility between both irrelevant dimensions occurred in CDCP and IDIP (the direction indicated was compatible with stimulus position). The N2pc amplitude was smaller in IDCP and CDIP than in CDCP and IDIP, what suggests that N2pc was modulated by S-S incompatibility and not by S-R incompatibilities. Copyright © 2013 Elsevier B.V. All rights reserved.

Metal Binding Properties of Escherichia coli YjiA, a Member of the Metal Homeostasis-Associated COG0523 Family of GTPases

PubMed Central

2013-01-01

GTPases are critical molecular switches involved in a wide range of biological functions. Recent phylogenetic and genomic analyses of the large, mostly uncharacterized COG0523 subfamily of GTPases revealed a link between some COG0523 proteins and metal homeostasis pathways. In this report, we detail the bioinorganic characterization of YjiA, a representative member of COG0523 subgroup 9 and the only COG0523 protein to date with high-resolution structural information. We find that YjiA is capable of binding several types of transition metals with dissociation constants in the low micromolar range and that metal binding affects both the oligomeric structure and GTPase activity of the enzyme. Using a combination of X-ray crystallography and site-directed mutagenesis, we identify, among others, a metal-binding site adjacent to the nucleotide-binding site in the GTPase domain that involves a conserved cysteine and several glutamate residues. Mutations of the coordinating residues decrease the impact of metal, suggesting that metal binding to this site is responsible for modulating the GTPase activity of the protein. These findings point toward a regulatory function for these COG0523 GTPases that is responsive to their metal-bound state. PMID:24449932

Novel members of quinoline compound family enhance insulin secretion in RIN-5AH beta cells and in rat pancreatic islet microtissue

PubMed Central

Orfi, Z.; Waczek, F.; Baska, F.; Szabadkai, I.; Torka, R.; Hartmann, J.; Orfi, L.; Ullrich, A.

2017-01-01

According to clinical data, some tyrosine kinase inhibitors (TKIs) possess antidiabetic effects. Several proposed mechanisms were assigned to them, however their mode of action is not clear. Our hypothesis was that they directly stimulate insulin release in beta cells. In our screening approach we demonstrated that some commercially available TKIs and many novel synthesized analogues were able to induce insulin secretion in RIN-5AH beta cells. Our aim was to find efficient, more selective and less toxic compounds. Out of several hits, we chose members from a compound family with quinoline core structure for further investigation. Here we present the studies done with these novel compounds and reveal structure activity relationships and mechanism of action. One of the most potent compounds (compound 9) lost its affinity to kinases, but efficiently increased calcium influx. In the presence of calcium channel inhibitors, the insulinotropic effect was attenuated or completely abrogated. While the quinoline TKI, bosutinib substantially inhibited tyrosine phosphorylation, compound 9 had no such effect. Molecular docking studies further supported our data. We confirmed that some TKIs possess antidiabetic effects, moreover, we present a novel compound family developed from the TKI, bosutinib and optimized for the modulation of insulin secretion. PMID:28272433

Imaging Erg and Jun transcription factor interaction in living cells using fluorescence resonance energy transfer analyses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Camuzeaux, Barbara; Spriet, Corentin; Heliot, Laurent

2005-07-15

Physical interactions between transcription factors play important roles in modulating gene expression. Previous in vitro studies have shown a transcriptional synergy between Erg protein, an Ets family member, and Jun/Fos heterodimer, members of the bZip family, which requires direct Erg-Jun protein interactions. Visualization of protein interactions in living cells is a new challenge in biology. For this purpose, we generated fusion proteins of Erg, Fos, and Jun with yellow and cyan fluorescent proteins, YFP and CFP, respectively. After transient expression in HeLa cells, interactions of the resulting fusion proteins were explored by fluorescence resonance energy transfer microscopy (FRET) in fixedmore » and living cells. FRET between YFP-Erg and CFP-Jun was monitored by using photobleaching FRET and fluorescence lifetime imaging microscopy. Both techniques revealed the occurrence of intermolecular FRET between YFP-Erg and CFP-Jun. This is stressed by loss of FRET with an YFP-Erg version carrying a point mutation in its ETS domain. These results provide evidence for the interaction of Erg and Jun proteins in living cells as a critical prerequisite of their transcriptional synergy, but also for the essential role of the Y371 residue, conserved in most Ets proteins, in this interaction.« less

SDDL- SOFTWARE DESIGN AND DOCUMENTATION LANGUAGE

NASA Technical Reports Server (NTRS)

Kleine, H.

1994-01-01

Effective, efficient communication is an essential element of the software development process. The Software Design and Documentation Language (SDDL) provides an effective communication medium to support the design and documentation of complex software applications. SDDL supports communication between all the members of a software design team and provides for the production of informative documentation on the design effort. Even when an entire development task is performed by a single individual, it is important to explicitly express and document communication between the various aspects of the design effort including concept development, program specification, program development, and program maintenance. SDDL ensures that accurate documentation will be available throughout the entire software life cycle. SDDL offers an extremely valuable capability for the design and documentation of complex programming efforts ranging from scientific and engineering applications to data management and business sytems. Throughout the development of a software design, the SDDL generated Software Design Document always represents the definitive word on the current status of the ongoing, dynamic design development process. The document is easily updated and readily accessible in a familiar, informative form to all members of the development team. This makes the Software Design Document an effective instrument for reconciling misunderstandings and disagreements in the development of design specifications, engineering support concepts, and the software design itself. Using the SDDL generated document to analyze the design makes it possible to eliminate many errors that might not be detected until coding and testing is attempted. As a project management aid, the Software Design Document is useful for monitoring progress and for recording task responsibilities. SDDL is a combination of language, processor, and methodology. The SDDL syntax consists of keywords to invoke design structures and a collection of directives which control processor actions. The designer has complete control over the choice of keywords, commanding the capabilities of the processor in a way which is best suited to communicating the intent of the design. The SDDL processor translates the designer's creative thinking into an effective document for communication. The processor performs as many automatic functions as possible, thereby freeing the designer's energy for the creative effort. Document formatting includes graphical highlighting of structure logic, accentuation of structure escapes and module invocations, logic error detection, and special handling of title pages and text segments. The SDDL generated document contains software design summary information including module invocation hierarchy, module cross reference, and cross reference tables of user selected words or phrases appearing in the document. The basic forms of the methodology are module and block structures and the module invocation statement. A design is stated in terms of modules that represent problem abstractions which are complete and independent enough to be treated as separate problem entities. Blocks are lower-level structures used to build the modules. Both kinds of structures may have an initiator part, a terminator part, an escape segment, or a substructure. The SDDL processor is written in PASCAL for batch execution on a DEC VAX series computer under VMS. SDDL was developed in 1981 and last updated in 1984.

Kinase inhibitors for CNS diseases: an analysis of the recent patent literature.

PubMed

Amigoni, Federica; Legnaghi, Elena; Pevarello, Paolo

2012-05-01

Protein kinases (PKs), as members of an important target class in current pharmaceutical research, have been mostly exploited so far in therapeutic areas such as oncology and inflammation. However, basic research on some PKs as key components of molecular mechanisms underlying neurodegeneration and neuroprotection may translate into new medicines for CNS diseases in the next few years. This review is an account of recent patents dealing with kinase inhibitors primarily designed for CNS indications. CNS-directed patents on kinase modulators published after 2008 were surveyed using SciFinder(®) and public patent search engines. Some PK targets, such as GSK-3β, CDK5, ROCK and p38α MAPK, continue to attract interest even though a clinical proof-of-concept is yet to be attained in a CNS setting. Less established PKs such as LRRK2, MLK, PAK and DAPK-1 hold promise as valuable targets of the future.

Interorganellar Regulation of Lysosome Positioning by the Golgi Apparatus through Rab34 Interaction with Rab-interacting Lysosomal Protein

PubMed Central

Wang, Tuanlao; Hong, Wanjin

2002-01-01

We present evidence to suggest the existence of a regulatory pathway for the Golgi apparatus to modulate the spatial positioning of otherwise distantly located lysosomes. Rab34, a new member of the Rab GTPase family, is associated primarily with the Golgi apparatus. Expression of wild-type or GTP-restricted but not GDP-restricted versions of Rab34 causes spatial redistribution of lysosomes from the periphery to the peri-Golgi region. The regulation of lysosomal positioning by Rab34 depends on its association with the membrane mediated by prenylation and its direct interaction with Rab-interacting lysosomal protein (RILP). This biological activity, mediated by Rab34-RILP interaction, is dependent on Lys82 in the switch I region. Our results have uncovered a novel mechanism for the Golgi apparatus to regulate the spatial distribution of another organelle. PMID:12475955

User Working Group Members

Atmospheric Science Data Center

2014-04-29

... the entire group may be directed to:  larc-asdc-uwg@lists.nasa.gov   Member Status Affiliation E-mail ... NASA Langley Research Center (NASA LaRC) takmeng.wong@nasa.gov Amy Braverman Member Jet Propulsion Laboratory (JPL) ...

Public perspectives on biospecimen procurement: what biorepositories should consider.

PubMed

L'Heureux, Jamie; Murray, Jeffrey C; Newbury, Elizabeth; Shinkunas, Laura; Simon, Christian M

2013-06-01

Human biospecimens are central to biobanking efforts, yet how members of the public think about biobank procurement strategies is not well understood. This study aimed to explore public perspectives toward the procurement of residual clinical material versus "direct" procurement strategies such as the drawing of blood. Members of the public residing in and beyond the biobank catchment area of the University of Iowa Hospitals and Clinics were randomly selected to participate in focus groups and a telephone survey. The majority of survey participants (75%, n=559) found both residual and direct procurement strategies equally workable. Small proportions preferred either residual (15%; n=117) or direct (5%; n=40) procurement. Focus group participants (n=48) could identify benefits to both procurement strategies, but raised concerns about possible donor inconvenience/discomfort and reduced biospecimen accrual in the case of direct procurement. Residual procurement raised concerns about lower-quality samples being procured without full donor awareness. Biobanks should consider that members of the public in their research programs may be willing to make specimen donations regardless of whether a residual or direct procurement strategy is employed. Limiting patient discomfort and inconvenience may make direct procurement strategies more acceptable to some members of the public. Ensuring donor awareness through effective informed consent may allay public concerns about the indirectness of donating clinical biospecimens.

Compact magnetic energy storage module

DOEpatents

Prueitt, M.L.

1994-12-20

A superconducting compact magnetic energy storage module in which a plurality of superconducting toroids, each having a toroidally wound superconducting winding inside a poloidally wound superconducting winding, are stacked so that the flow of electricity in each toroidally wound superconducting winding is in a direction opposite from the direction of electrical flow in other contiguous superconducting toroids. This allows for minimal magnetic pollution outside of the module. 4 figures.

Compact magnetic energy storage module

DOEpatents

Prueitt, Melvin L.

1994-01-01

A superconducting compact magnetic energy storage module in which a plurality of superconducting toroids, each having a toroidally wound superconducting winding inside a poloidally wound superconducting winding, are stacked so that the flow of electricity in each toroidally wound superconducting winding is in a direction opposite from the direction of electrical flow in other contiguous superconducting toroids. This allows for minimal magnetic pollution outside of the module.

Functional and transcriptome analysis reveals an acclimatization strategy for abiotic stress tolerance mediated by Arabidopsis NF-YA family members.

PubMed

Leyva-González, Marco Antonio; Ibarra-Laclette, Enrique; Cruz-Ramírez, Alfredo; Herrera-Estrella, Luis

2012-01-01

Nuclear Factor Y (NF-Y) is a heterotrimeric complex formed by NF-YA/NF-YB/NF-YC subunits that binds to the CCAAT-box in eukaryotic promoters. In contrast to other organisms, in which a single gene encodes each subunit, in plants gene families of over 10 members encode each of the subunits. Here we report that five members of the Arabidopsis thaliana NF-YA family are strongly induced by several stress conditions via transcriptional and miR169-related post-transcriptional mechanisms. Overexpression of NF-YA2, 7 and 10 resulted in dwarf late-senescent plants with enhanced tolerance to several types of abiotic stress. These phenotypes are related to alterations in sucrose/starch balance and cell elongation observed in NF-YA overexpressing plants. The use of transcriptomic analysis of transgenic plants that express miR169-resistant versions of NF-YA2, 3, 7, and 10 under an estradiol inducible system, as well as a dominant-repressor version of NF-YA2 revealed a set of genes, whose promoters are enriched in NF-Y binding sites (CCAAT-box) and that may be directly regulated by the NF-Y complex. This analysis also suggests that NF-YAs could participate in modulating gene regulation through positive and negative mechanisms. We propose a model in which the increase in NF-YA transcript levels in response to abiotic stress is part of an adaptive response to adverse environmental conditions in which a reduction in plant growth rate plays a key role.

Functional and Transcriptome Analysis Reveals an Acclimatization Strategy for Abiotic Stress Tolerance Mediated by Arabidopsis NF-YA Family Members

PubMed Central

Leyva-González, Marco Antonio; Ibarra-Laclette, Enrique; Cruz-Ramírez, Alfredo; Herrera-Estrella, Luis

2012-01-01

Nuclear Factor Y (NF-Y) is a heterotrimeric complex formed by NF-YA/NF-YB/NF-YC subunits that binds to the CCAAT-box in eukaryotic promoters. In contrast to other organisms, in which a single gene encodes each subunit, in plants gene families of over 10 members encode each of the subunits. Here we report that five members of the Arabidopsis thaliana NF-YA family are strongly induced by several stress conditions via transcriptional and miR169-related post-transcriptional mechanisms. Overexpression of NF-YA2, 7 and 10 resulted in dwarf late-senescent plants with enhanced tolerance to several types of abiotic stress. These phenotypes are related to alterations in sucrose/starch balance and cell elongation observed in NF-YA overexpressing plants. The use of transcriptomic analysis of transgenic plants that express miR169-resistant versions of NF-YA2, 3, 7, and 10 under an estradiol inducible system, as well as a dominant-repressor version of NF-YA2 revealed a set of genes, whose promoters are enriched in NF-Y binding sites (CCAAT-box) and that may be directly regulated by the NF-Y complex. This analysis also suggests that NF-YAs could participate in modulating gene regulation through positive and negative mechanisms. We propose a model in which the increase in NF-YA transcript levels in response to abiotic stress is part of an adaptive response to adverse environmental conditions in which a reduction in plant growth rate plays a key role. PMID:23118940

Concentrator photovoltaic module architectures with capabilities for capture and conversion of full global solar radiation

PubMed Central

Lee, Kyu-Tae; Yao, Yuan; He, Junwen; Fisher, Brent; Sheng, Xing; Lumb, Matthew; Xu, Lu; Anderson, Mikayla A.; Scheiman, David; Han, Seungyong; Kang, Yongseon; Gumus, Abdurrahman; Bahabry, Rabab R.; Lee, Jung Woo; Paik, Ungyu; Bronstein, Noah D.; Alivisatos, A. Paul; Meitl, Matthew; Burroughs, Scott; Hussain, Muhammad Mustafa; Lee, Jeong Chul; Nuzzo, Ralph G.; Rogers, John A.

2016-01-01

Emerging classes of concentrator photovoltaic (CPV) modules reach efficiencies that are far greater than those of even the highest performance flat-plate PV technologies, with architectures that have the potential to provide the lowest cost of energy in locations with high direct normal irradiance (DNI). A disadvantage is their inability to effectively use diffuse sunlight, thereby constraining widespread geographic deployment and limiting performance even under the most favorable DNI conditions. This study introduces a module design that integrates capabilities in flat-plate PV directly with the most sophisticated CPV technologies, for capture of both direct and diffuse sunlight, thereby achieving efficiency in PV conversion of the global solar radiation. Specific examples of this scheme exploit commodity silicon (Si) cells integrated with two different CPV module designs, where they capture light that is not efficiently directed by the concentrator optics onto large-scale arrays of miniature multijunction (MJ) solar cells that use advanced III–V semiconductor technologies. In this CPV+ scheme (“+” denotes the addition of diffuse collector), the Si and MJ cells operate independently on indirect and direct solar radiation, respectively. On-sun experimental studies of CPV+ modules at latitudes of 35.9886° N (Durham, NC), 40.1125° N (Bondville, IL), and 38.9072° N (Washington, DC) show improvements in absolute module efficiencies of between 1.02% and 8.45% over values obtained using otherwise similar CPV modules, depending on weather conditions. These concepts have the potential to expand the geographic reach and improve the cost-effectiveness of the highest efficiency forms of PV power generation. PMID:27930331

Concentrator photovoltaic module architectures with capabilities for capture and conversion of full global solar radiation

DOE PAGES

Lee, Kyu-Tae; Yao, Yuan; He, Junwen; ...

2016-12-05

Emerging classes ofconcentrator photovoltaic (CPV) modules reach efficiencies that are far greater than those of even the highest performance flat-plate PV technologies, with architectures that have the potential to provide the lowest cost of energy in locations with high direct normal irradiance (DNI). A disadvantage is their inability to effectively use diffuse sunlight, thereby constraining widespread geographic deployment and limiting performance even under the most favorable DNI conditions. This study introduces a module design that integrates capabilities in flat-plate PV directly with the most sophisticated CPV technologies, for capture of both direct and diffuse sunlight, thereby achieving efficiency in PVmore » conversion of the global solar radiation. Specific examples of this scheme exploit commodity silicon (Si) cells integrated with two different CPV module designs, where they capture light that is not efficiently directed by the concentrator optics onto large-scale arrays of miniature multijunction (MJ) solar cells that use advanced III-V semiconductor technologies. In this CPV + scheme ("+" denotes the addition of diffuse collector), the Si and MJ cells operate independently on indirect and direct solar radiation, respectively. On-sun experimental studies of CPV + modules at latitudes of 35.9886° N (Durham, NC), 40.1125° N (Bondville, IL), and 38.9072° N (Washington, DC) show improvements in absolute module efficiencies of between 1.02% and 8.45% over values obtained using otherwise similar CPV modules, depending on weather conditions. These concepts have the potential to expand the geographic reach and improve the cost-effectiveness of the highest efficiency forms of PV power generation.« less

Concentrator photovoltaic module architectures with capabilities for capture and conversion of full global solar radiation

NASA Astrophysics Data System (ADS)

Lee, Kyu-Tae; Yao, Yuan; He, Junwen; Fisher, Brent; Sheng, Xing; Lumb, Matthew; Xu, Lu; Anderson, Mikayla A.; Scheiman, David; Han, Seungyong; Kang, Yongseon; Gumus, Abdurrahman; Bahabry, Rabab R.; Lee, Jung Woo; Paik, Ungyu; Bronstein, Noah D.; Alivisatos, A. Paul; Meitl, Matthew; Burroughs, Scott; Mustafa Hussain, Muhammad; Lee, Jeong Chul; Nuzzo, Ralph G.; Rogers, John A.

2016-12-01

Emerging classes of concentrator photovoltaic (CPV) modules reach efficiencies that are far greater than those of even the highest performance flat-plate PV technologies, with architectures that have the potential to provide the lowest cost of energy in locations with high direct normal irradiance (DNI). A disadvantage is their inability to effectively use diffuse sunlight, thereby constraining widespread geographic deployment and limiting performance even under the most favorable DNI conditions. This study introduces a module design that integrates capabilities in flat-plate PV directly with the most sophisticated CPV technologies, for capture of both direct and diffuse sunlight, thereby achieving efficiency in PV conversion of the global solar radiation. Specific examples of this scheme exploit commodity silicon (Si) cells integrated with two different CPV module designs, where they capture light that is not efficiently directed by the concentrator optics onto large-scale arrays of miniature multijunction (MJ) solar cells that use advanced III-V semiconductor technologies. In this CPV+ scheme (“+” denotes the addition of diffuse collector), the Si and MJ cells operate independently on indirect and direct solar radiation, respectively. On-sun experimental studies of CPV+ modules at latitudes of 35.9886° N (Durham, NC), 40.1125° N (Bondville, IL), and 38.9072° N (Washington, DC) show improvements in absolute module efficiencies of between 1.02% and 8.45% over values obtained using otherwise similar CPV modules, depending on weather conditions. These concepts have the potential to expand the geographic reach and improve the cost-effectiveness of the highest efficiency forms of PV power generation.

Concentrator photovoltaic module architectures with capabilities for capture and conversion of full global solar radiation.

PubMed

Lee, Kyu-Tae; Yao, Yuan; He, Junwen; Fisher, Brent; Sheng, Xing; Lumb, Matthew; Xu, Lu; Anderson, Mikayla A; Scheiman, David; Han, Seungyong; Kang, Yongseon; Gumus, Abdurrahman; Bahabry, Rabab R; Lee, Jung Woo; Paik, Ungyu; Bronstein, Noah D; Alivisatos, A Paul; Meitl, Matthew; Burroughs, Scott; Hussain, Muhammad Mustafa; Lee, Jeong Chul; Nuzzo, Ralph G; Rogers, John A

2016-12-20

Emerging classes of concentrator photovoltaic (CPV) modules reach efficiencies that are far greater than those of even the highest performance flat-plate PV technologies, with architectures that have the potential to provide the lowest cost of energy in locations with high direct normal irradiance (DNI). A disadvantage is their inability to effectively use diffuse sunlight, thereby constraining widespread geographic deployment and limiting performance even under the most favorable DNI conditions. This study introduces a module design that integrates capabilities in flat-plate PV directly with the most sophisticated CPV technologies, for capture of both direct and diffuse sunlight, thereby achieving efficiency in PV conversion of the global solar radiation. Specific examples of this scheme exploit commodity silicon (Si) cells integrated with two different CPV module designs, where they capture light that is not efficiently directed by the concentrator optics onto large-scale arrays of miniature multijunction (MJ) solar cells that use advanced III-V semiconductor technologies. In this CPV + scheme ("+" denotes the addition of diffuse collector), the Si and MJ cells operate independently on indirect and direct solar radiation, respectively. On-sun experimental studies of CPV + modules at latitudes of 35.9886° N (Durham, NC), 40.1125° N (Bondville, IL), and 38.9072° N (Washington, DC) show improvements in absolute module efficiencies of between 1.02% and 8.45% over values obtained using otherwise similar CPV modules, depending on weather conditions. These concepts have the potential to expand the geographic reach and improve the cost-effectiveness of the highest efficiency forms of PV power generation.

Raffaello Multi-Purpose Logistics Module (MPLM) in the Endeavour payload bay prior to docking

NASA Image and Video Library

2001-04-21

ISS002-E-5815 (21 April 2001) --- The Raffaello Multi-Purpose Logistics Module (MPLM), built by the Italian Space Agency (ASI), sits in its berthed position in the cargo bay of the Space Shuttle Endeavour as the STS-100 crew eases the vehicle close to the International Space Station (ISS) for docking. The image was recorded with a digital still camera by one of the Expedition Two crew members aboard the Station.

U.S. Secretary of State addresses launch team

NASA Technical Reports Server (NTRS)

1998-01-01

In a firing room of the Launch Control Center, U.S. Secretary of State Madeleine Albright speaks to the launch team after the successful launch of Space Shuttle Endeavour at 3:35:34 a.m. EST. During the nearly 12-day mission of STS-88, the six-member crew will mate in space the first two elements of the International Space Station -- the already-orbiting Zarya control module and the Unity connecting module carried by Endeavour.

U.S. Secretary of State addresses launch team

NASA Technical Reports Server (NTRS)

1998-01-01

In a firing room of the Launch Control Center,U.S. Secretary of State Madeleine Albright waves to the personnel after her speech about the successful launch of Space Shuttle Endeavour. At her right is NASA Administrator Daniel Goldin. During the nearly 12- day mission of STS-88, the six-member crew will mate in space the first two elements of the International Space Station -- the already-orbiting Zarya control module and the Unity connecting module carried by Endeavour.

The Water Framework Directive: The Challenges of Testing and Validation of Guidance Documents

NASA Astrophysics Data System (ADS)

Barth, F.; Bidoglio, G.; Murray, C. N.; Zaldivar, J.; Bouraoui, F.

On the 23rd October 2000 the European Parliament and Council passed a Directive establishing a framework of community action in the field of water policy (Water Framework Directive- FWD). The Water Framework Directive (FWD) raises major challenges, these include an extremely demanding timetable, in particular in the nine preparatory years; the complexity of the text and the diversity of possible solutions to scientific, technical and practical questions. A further problem is that a common understanding and methodologies for the application of the different areas of the FWD do not necessarily exist. Member States have, historically, developed approaches to monitoring, impact assessment, economic analysis etc. that will need to be compared in order to be certain that they provide comparable level of results over the range of ecosystems covered in the European Union. Accession Countries will also have to start to adjust their environmental legislation to be compatible with EU Directives and standards. The Framework Water Directive imposes a series of deadlines for the reporting by Member States to the European Commission. In order to respond to this problem a Common Strategy on the Implementation of the Water Framework Directive is being developed by the European Commission and Member States. The aim of the development of this Common Strategy is to allow, as far as possible, a coherent and harmonious implementation of the Directive. Focus is on methodological questions related to a common understanding of the technical and scientific implications of the Directive. The aim is to clarify and develop, where appropriate, supporting technical and scientific information to assist in the practical implementation of the Directive. Guidance documents, advice for operational methods and other supporting documents will be developed for this purpose. A modular structure has been chosen for the overall strategy. The main modules are the key activities for the implementation process.. · Activity 1: Information sharing · Activity 2: Develop guidance on technical issues · Activity 3: Information and data management · Activity 4: Application, testing and validation The first three priorities have a more horizontal character. They are the key activities for developing a common understanding of the implementation of the Water Framework Directive. All these horizontal activities need to be integrated and made operational in the River Basin Management Plans. Activity 4 (Application, Testing and Validation) significantly contributes to this integration role by making these activities operational in the River Basin Management Plans. The integration step is crucial for the effective implementation of the WFD. The objective of Activity 4 is to ensure coherence amongst the different guidance documents and their cross applicability by testing the guidance documents in selected pilot river basins. To achieve these objectives a Network of pilot river basins and associated coastal zones (where applicable) will be identified, in close co- operation with WGs in Key Action 2, that are considered to represent a range of problems and conditions characteristic of those to be found in the application of the different guidelines. The Network of identified sites will used for testing and cross- validation of proposed WG guidelines. The Joint Research Centre is acting as the technical secretariat for the Scientific Coordination Committee who is responsible for Activity 4. The purpose of the present paper is to describe approach, methodology and timetable for integrated testing of guidance documents.

KSC-99pp1499

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- At SPACEHAB, in Titusville, Fla., STS-101 crew members take part in a Crew Equipment Interface Test (CEIT). Here they are checking out the SPACEHAB Logistics Double Module. The crew is composed of Commander James Donald Halsell Jr., Pilot Scott J. "Doc" Horowitz (Ph.D.), and Mission Specialists Mary Ellen Weber (Ph.D.), Edward Tsang Lu (Ph.D.), Jeffrey N. Williams, and Yuri Malenchenko and Boris W. Morukov, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1502

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- At SPACEHAB, in Titusville, Fla., STS-101 Mission Specialists Edward Tsang Lu (Ph.D.), Mary Ellen Weber (Ph.D.) and Boris W. Morukov, who is with the Russian Space Agency (RSA), stand inside the SPACEHAB Logistics Double Module, part of the payload for their mission. They and other crew members Commander James Donald Halsell Jr., Pilot Scott J. "Doc" Horowitz (Ph.D.), and Mission Specialists Jeffrey N. Williams, and Yuri Malenchenko (also with RSA), are taking part in a Crew Equipment Interface Test. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1501

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- At SPACEHAB, in Titusville, Fla., STS-101 crew members take part in a Crew Equipment Interface Test (CEIT). Here they are checking out the SPACEHAB Logistics Double Module. The crew is composed of Commander James Donald Halsell Jr., Pilot Scott J. "Doc" Horowitz (Ph.D.), and Mission Specialists Mary Ellen Weber (Ph.D.), Edward Tsang Lu (Ph.D.), Jeffrey N. Williams, and Yuri Malenchenko and Boris W. Morukov, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

KSC-99pp1496

NASA Image and Video Library

1999-12-10

KENNEDY SPACE CENTER, FLA. -- During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Titusville, Fla., STS-101 crew members check out the SPACEHAB Logistics Double Module that will be part of the payload for their mission. The crew is composed of Commander James Donald Halsell Jr., Pilot Scott J. "Doc" Horowitz (Ph.D.), and Mission Specialists Mary Ellen Weber (Ph.D.), Edward Tsang Lu (Ph.D.), Jeffrey N. Williams, and Yuri Malenchenko and Boris W. Morukov, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

During a Crew Equipment Interface Test (CEIT) at SPACEHAB, in Titusville, Fla., STS-101 crew members check out the SPACEHAB Logistics Double Module that will be part of the payload for their mission. The crew is composed of Commander James Donald Halsell Jr., Pilot Scott J. 'Doc' Horowitz (Ph.D.), and Mission Specialists Mary Ellen Weber (Ph.D.), Edward Tsang Lu (Ph.D.), Jeffrey N. Williams, and Yuri Malenchenko and Boris W. Morukov, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

At SPACEHAB, in Titusville, Fla., STS-101 crew members take part in a Crew Equipment Interface Test (CEIT). Here they are checking out the SPACEHAB Logistics Double Module. The crew is composed of Commander James Donald Halsell Jr., Pilot Scott J. 'Doc' Horowitz (Ph.D.), and Mission Specialists Mary Ellen Weber (Ph.D.), Edward Tsang Lu (Ph.D.), Jeffrey N. Williams, and Yuri Malenchenko and Boris W. Morukov, who are with the Russian Space Agency. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

STS-101 crew take part in CEIT at SPACEHAB

NASA Technical Reports Server (NTRS)

1999-01-01

At SPACEHAB, in Titusville, Fla., STS-101 Mission Specialists Edward Tsang Lu (Ph.D.), Mary Ellen Weber (Ph.D.) and Boris W. Morukov, who is with the Russian Space Agency (RSA), stand inside the SPACEHAB Logistics Double Module, part of the payload for their mission. They and other crew members Commander James Donald Halsell Jr., Pilot Scott J. 'Doc' Horowitz (Ph.D.), and Mission Specialists Jeffrey N. Williams, and Yuri Malenchenko (also with RSA), are taking part in a Crew Equipment Interface Test. The primary objective of the STS-101 mission is to complete the initial outfitting of the International Space Station, making it fully ready for the first long-term crew. The seven-member crew will transfer almost two tons of equipment and supplies from SPACEHAB. Additionally, they will unpack a shipment of supplies delivered earlier by a Russian Progress space tug and begin outfitting the newly arrived Zvezda Service Module. Three astronauts will perform two space walks to transfer and install parts of the Russian Strela cargo boom that are attached to SPACEHAB's Integrated Cargo Container, connect utility cables between Zarya and Zvezda, and install a magnetometer/pole assembly on the Service Module. Additional activities for the STS-101 astronauts include working with the Space Experiment Module (SEM-06) and the Mission to America's Remarkable Schools (MARS), two educational initiatives. STS-101 is scheduled for launch no earlier than March 16, 2000.

Presence of claudins mRNA in the brain. Selective modulation of expression by kindling epilepsy.

PubMed

Lamas, Mónica; González-Mariscal, Lorenza; Gutiérrez, Rafael

2002-08-15

In the central nervous system, the junctional types that establish and maintain tissue architecture include gap junctions, for cytoplasmic connectivity, and tight junctions, for paracellular and/or cell polarity barriers. Connexins are the integral membrane proteins of gap junctions, whereas occludin and members of the multigene family of claudins form tight junctions. In the brain, there are no transendothelial pathways, as continuous tight junctions are present between the endothelial cells. Thus, they provide a continuous cellular barrier between the blood and the insterstitial fluid. However, several brain pathologies, including epilepsy, are known to alter the permeability of the blood-brain barrier and to cause edema. Therefore, since claudins, as constitutive proteins of tight junctions are likely candidates for modulation under pathological states, we explored their normal pattern of expression in the brain and its modulation by seizures. We found that several members of this family are normally expressed in the hippocampus and cortex. Interestingly, claudin-7 is expressed in the hippocampus but not in the cortex. On the other hand, the expression of claudin-8 is selectively down-regulated in the hippocampus as kindling evolves. These results link for the first time the modulation of expression of a tight junction protein to abnormal neuronal synchronization that could probably be reflected in permeability changes of the blood-brain barrier or edema.

The topoisomerase II-Hsp90 complex: a new chemotherapeutic target?

PubMed

Barker, Catherine R; Hamlett, Jane; Pennington, Stephen R; Burrows, Francis; Lundgren, Karen; Lough, Rachel; Watson, Alastair J M; Jenkins, John R

2006-06-01

The modulation of DNA topology by topoisomerase II plays a crucial role during chromosome condensation and segregation in mitosis and has thus become a highly attractive target for chemotherapeutic drugs. However, these drugs are highly toxic, and so new approaches are required. One such strategy is to target topoisomerase II-interacting proteins. Here we report the identification of potential topoisomerase II-associated proteins using immunoprecipitation, followed by 1-D and 2-D gel electrophoresis and MALDI-TOF mass spectrometry. A total of 23 proteins were identified and, of these, 17 were further validated as topoisomerase IIalpha-associated proteins by coimmunoprecipitation and Western blot. Six of the interacting proteins were cellular chaperones, including 3 members of the heat shock protein-90 (Hsp90) family, and so the effect of Hsp90 modulation on the antitumor activity of topoisomerase II drugs was tested using the sulforhodamine B assay, clonogenic assays and a xenograft model. The Hsp90 inhibitors geldanamycin, 17-AAG (17-allylamino-17-demethoxygeldanamycin) and radicicol significantly enhanced the activity of the topoisomerase II poisons etoposide and mitoxantrone in vitro and in vivo. Thus, our method of identifying topoisomerase II-interacting proteins appears to be effective, and at least 1 novel topoisomerase IIalpha-associated protein, Hsp90, may represent a valid drug target in the context of topoisomerase II-directed chemotherapy.

Induction of therapeutic hypothermia by pharmacological modulation of temperature-sensitive TRP channels: theoretical framework and practical considerations.

PubMed

Feketa, Viktor V; Marrelli, Sean P

2015-01-01

Therapeutic hypothermia has emerged as a remarkably effective method of neuroprotection from ischemia and is being increasingly used in clinics. Accordingly, it is also a subject of considerable attention from a basic scientific research perspective. One of the fundamental problems, with which current studies are concerned, is the optimal method of inducing hypothermia. This review seeks to provide a broad theoretical framework for approaching this problem, and to discuss how a novel promising strategy of pharmacological modulation of the thermosensitive ion channels fits into this framework. Various physical, anatomical, physiological and molecular aspects of thermoregulation, which provide the foundation for this text, have been comprehensively reviewed and will not be discussed exhaustively here. Instead, the first part of the current review, which may be helpful for a broader readership outside of thermoregulation research, will build on this existing knowledge to outline possible opportunities and research directions aimed at controlling body temperature. The second part, aimed at a more specialist audience, will highlight the conceptual advantages and practical limitations of novel molecular agents targeting thermosensitive Transient Receptor Potential (TRP) channels in achieving this goal. Two particularly promising members of this channel family, namely TRP melastatin 8 (TRPM8) and TRP vanilloid 1 (TRPV1), will be discussed in greater detail.

Construction of the Zeus forward/rear calorimeter modules at NIKHEF

NASA Astrophysics Data System (ADS)

Blankers, R.; Engelen, J.; Geerinck, H.; Homma, J.; Hunck, P.; Dekoning, N.; Kooijman, P.; Korporaal, A.; Loos, R.; Straver, J.

1990-07-01

The design and assembly procedure of the FCAL/RCAL (Forward (in proton direction) Calorimeter/Rear (in electron direction) Calorimeter) of the Zeus detector to study electron proton interactions at Desy, Hamburg (Germany, F.R.) are detailed. The main components of the modules are described: steel C-frame which provides the overall mechanical module structure; a stack of depleted uranium plates and scintillator plates; wavelength shifter material, mounted in cassettes for the readout of the scintillator light; stainless steel straps which compress the stack and fix it to the C-frame. Finite element techniques for module force calculations are outlined. The module assembly and transport and calibration tools are described.

Improved Portable Ultrasonic Leak Detectors

NASA Technical Reports Server (NTRS)

Youngquist, Robert C.; Moerk, John S.; Haskell, William D.; Cox, Robert B.; Polk, Jimmy D.; Strobel, James P.; Luaces, Frank

1995-01-01

Improved portable ultrasonic leak detector features three interchangeable ultrasonic-transducer modules, each suited for operation in unique noncontact or contact mode. One module equipped with ultrasound-collecting horn for use in scanning to detect leaks from distance; horn provides directional sensitivity pattern with sensitivity multiplied by factor of about 6 in forward direction. Another module similar, does not include horn; this module used for scanning close to suspected leak, where proximity of leak more than offsets loss of sensitivity occasioned by lack of horn. Third module designed to be pressed against leaking vessel; includes rugged stainless-steel shell. Improved detectors perform significantly better, smaller, more rugged, and greater sensitivity.

MORAL ENHANCEMENT VIA DIRECT EMOTION MODULATION: A REPLY TO JOHN HARRIS

PubMed Central

Douglas, Thomas

2013-01-01

Some argue that humans should enhance their moral capacities by adopting institutions that facilitate morally good motives and behaviour. I have defended a parallel claim: that we could permissibly use biomedical technologies to enhance our moral capacities, for example by attenuating certain counter-moral emotions. John Harris has recently responded to my argument by raising three concerns about the direct modulation of emotions as a means to moral enhancement. He argues (1) that such means will be relatively ineffective in bringing about moral improvements, (2) that direct modulation of emotions would invariably come at an unacceptable cost to our freedom, and (3) that we might end up modulating emotions in ways that actually lead to moral decline. In this article I outline some counter-intuitive potential implications of Harris' claims. I then respond individually to his three concerns, arguing that they license only the very weak conclusion that moral enhancement via direct emotion modulation is sometimes impermissible. However I acknowledge that his third concern might, with further argument, be developed into a more troubling objection to such enhancements. PMID:22092503

Moral enhancement via direct emotion modulation: a reply to John Harris.

PubMed

Douglas, Thomas

2013-03-01

Some argue that humans should enhance their moral capacities by adopting institutions that facilitate morally good motives and behaviour. I have defended a parallel claim: that we could permissibly use biomedical technologies to enhance our moral capacities, for example by attenuating certain counter-moral emotions. John Harris has recently responded to my argument by raising three concerns about the direct modulation of emotions as a means to moral enhancement. He argues (1) that such means will be relatively ineffective in bringing about moral improvements, (2) that direct modulation of emotions would invariably come at an unacceptable cost to our freedom, and (3) that we might end up modulating emotions in ways that actually lead to moral decline. In this article I outline some counter-intuitive potential implications of Harris' claims. I then respond individually to his three concerns, arguing that they license only the very weak conclusion that moral enhancement via direct emotion modulation is sometimes impermissible. However I acknowledge that his third concern might, with further argument, be developed into a more troubling objection to such enhancements. © 2011 Blackwell Publishing Ltd.

Structural basis for the ATP-independent proteolytic activity of LonB proteases and reclassification of their AAA+ modules.

PubMed

An, Young Jun; Na, Jung-Hyun; Kim, Myung-Il; Cha, Sun-Shin

2015-10-01

Lon proteases degrade defective or denature proteins as well as some folded proteins for the control of cellular protein quality. There are two types of Lon proteases, LonA and LonB. Each consists of two functional components: a protease component and an ATPase associated with various cellular activities (AAA+ module). Here, we report the 2.03 -resolution crystal structure of the isolated AAA+ module (iAAA+ module) of LonB from Thermococcus onnurineus NA1 (TonLonB). The iAAA+ module, having no bound nucleotide, adopts a conformation virtually identical to the ADP-bound conformation of AAA+ modules in the hexameric structure of TonLonB; this provides insights into the ATP-independent proteolytic activity observed in a LonB protease. Structural comparison of AAA+ modules between LonA and LonB revealed that the AAA+ modules of Lon proteases are separated into two distinct clades depending on their structural features. The AAA+ module of LonB belongs to the -H2 & Ins1 insert clade (HINS clade)- defined for the first time in this study, while the AAA+ module of LonA is a member of the HCLR clade.

Expedition One crew in Russian with Service Module

NASA Image and Video Library

2000-07-14

Photographic documentation of Expedition One crew in Russia with Service Module. Views include: The three crew members for ISS Expedition One train with computers on the trainer / mockup for the Zvezda Service Module. From the left are cosmonauts Yuri Gidzenko, Soyuz commander; and Sergei Krikalev, flight engineer; and astronaut William Shepherd, mission commander. The session took place at the Gagarin Cosmonaut Training Center in Russia (18628). View looking toward the hatch inside the Zvezda Service Module trainer / mockup at the Gagarin Cosmonaut Training Center in Russia (18629). A wide shot of the Zvezda Service Module trainer / mockup, with the transfer compartment in the foreground (18630). Side view of the Zvezda Service Module (18631). An interior shot of the Zarya / Functional Cargo Bay (FGB) trainer / mockup (18632). Astronaut Scott Kelly, director of operations - Russia, walks through a full scale trainer / mockup for the Zvezda Service Module at the Gagarin Cosmonaut Training Center in Russia (18633). Astronaut William Shepherd (right) mission commander for ISS Expedition One, and Sergei Krikalev, flight engineer, participate in a training session in a trainer / mockup of the Zvezda Service Module (18634).

STS-107 Crew Equipment Interface Test (CEIT)activities at SPACEHAB

NASA Technical Reports Server (NTRS)

2001-01-01

KENNEDY SPACE CENTER, Fla. -- At SPACEHAB, Cape Canaveral, Fla., members of the STS-107 crew discuss the experiments in the Spacehab module. Seated, in the foreground, is Mission Specialist Laurel Blair Salton Clark; standing behind her are Commander Rick Douglas Husband and Mission Specialist Kalpana Chawla. They and other crew members Pilot William C. McCool; Payload Commander Michael P. Anderson; and Mission Specialists David M. Brown and Ilan Ramon, of Israel, are at SPACEHAB for Crew Equipment Interface Test (CEIT) activities. The CEIT enables the crew to perform certain flight operations, operate experiments in a flight-like environment, evaluate stowage locations and obtain additional exposure to specific experiment operations. As a research mission, STS-107 will carry the Spacehab Double Module in its first research flight into space and a broad collection of experiments ranging from material science to life science. STS-107 is scheduled for launch May 23, 2002

STS-107 Crew Equipment Interface Test (CEIT)activities at SPACEHAB

NASA Technical Reports Server (NTRS)

2001-01-01

KENNEDY SPACE CENTER, Fla. -- At SPACEHAB, Cape Canaveral, Fla., STS-107 Payload Specialist Ilan Ramon (foreground), of Israel, and Mission Specialist Kalpana Chawla (background) check out experiments inside the Spacehab module. They and other crew members are taking part in Crew Equipment Interface Test (CEIT) activities that enable the crew to perform certain flight operations, operate experiments in a flight-like environment, evaluate stowage locations and obtain additional exposure to specific experiment operations. As a research mission, STS-107 will carry the Spacehab Double Module in its first research flight into space and a broad collection of experiments ranging from material science to life science. . Other STS-107 crew members are Commander Rick Douglas Husband, Pilot William C. McCool; Payload Commander Michael P. Anderson; and Mission Specialists Laurel Blair Salton Clark and David M. Brown. STS-107 is scheduled for launch May 23, 2002

STS-107 Crew Equipment Interface Test (CEIT)activities at SPACEHAB

NASA Technical Reports Server (NTRS)

2001-01-01

KENNEDY SPACE CENTER, Fla. -- STS-107 Payload Specialist Ilan Ramon, of Israel, manipulates a piece of equipment in the Spacehab module. He and other crew members are taking part in Crew Equipment Interface Test (CEIT) activities at SPACEHAB, Cape Canaveral, Fla. As a research mission, STS-107 will carry the Spacehab Double Module in its first research flight into space and a broad collection of experiments ranging from material science to life science. The CEIT activities enable the crew to perform certain flight operations, operate experiments in a flight-like environment, evaluate stowage locations and obtain additional exposure to specific experiment operations. Other STS-107 crew members are Commander Rick Douglas Husband, Pilot William C. McCool; Payload Commander Michael P. Anderson; and Mission Specialists Kalpana Chawla, Laurel Blair Salton Clark and David M. Brown. STS-107 is scheduled for launch May 23, 2002

KENNEDY SPACE CENTER, FLA. - - In the Space Station Processing Facility, STS-114 Mission Specialist Stephen Robinson (left) learns about the Japanese Experiment Module (JEM) from Jennifer Goldsmith (center), with United Space Alliance at Johnson Space Center, and Louise Kleba (right), with USA at KSC. Crew members are at KSC to become familiar with Shuttle and mission equipment. The mission is Logistics Flight 1, which is scheduled to deliver supplies and equipment plus the external stowage platform to the International Space Station.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - - In the Space Station Processing Facility, STS-114 Mission Specialist Stephen Robinson (left) learns about the Japanese Experiment Module (JEM) from Jennifer Goldsmith (center), with United Space Alliance at Johnson Space Center, and Louise Kleba (right), with USA at KSC. Crew members are at KSC to become familiar with Shuttle and mission equipment. The mission is Logistics Flight 1, which is scheduled to deliver supplies and equipment plus the external stowage platform to the International Space Station.

KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, STS-114 Mission Specialist Stephen Robinson (right) learns about the Japanese Experiment Module (JEM) from Louise Kleba (left), with United Space Alliance at KSC, and Jennifer Goldsmith (center), with USA at Johnson Space Center. Crew members are at KSC becoming familiar with Shuttle and mission equipment. The mission is Logistics Flight 1, which is scheduled to deliver supplies and equipment plus the external stowage platform to the International Space Station.

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, STS-114 Mission Specialist Stephen Robinson (right) learns about the Japanese Experiment Module (JEM) from Louise Kleba (left), with United Space Alliance at KSC, and Jennifer Goldsmith (center), with USA at Johnson Space Center. Crew members are at KSC becoming familiar with Shuttle and mission equipment. The mission is Logistics Flight 1, which is scheduled to deliver supplies and equipment plus the external stowage platform to the International Space Station.

From Modules to a Generator: An Integrated Heat Exchanger Concept for Car Applications of a Thermoelectric Generator

NASA Astrophysics Data System (ADS)

Bosch, Henry

2016-03-01

A heat exchanger concept for a thermoelectric generator with integrated planar modules for passenger car applications is introduced. The module housings, made of deep drawn stainless steel sheet metal, are brazed onto the exhaust gas channel to achieve an optimal heat transfer on the hot side of the modules. The cooling side consists of winding fluid channels, which are mounted directly onto the cold side of the modules. Only a thin foil separates the cooling media from the modules for an almost direct heat contact on the cooling side. Thermoelectric generators with up to 20 modules made of PbTe and Bi2Te3, respectively, are manufactured and tested on a hot gas generator to investigate electrical power output and performance of the thermoelectric generator. The proof of concept of the light weight heat exchanger design made of sheet metal with integrated modules is positively accomplished.

Amorphous-silicon module intercell corrosion

NASA Astrophysics Data System (ADS)

Mon, G. R.; Ross, R. G.

1987-06-01

Three non-electrochemical, moisture-induced a-Si module degradation modes have been observed and their mechanisms studied: (1) the formation and growth of pinholes in the thin-film layers; (2) the directional interfusion of pinholes along process scribe lines to form metallization-free regions that tend to open-circuit the module; and (3) worm-like filiform corrosion in the aluminum layer. The dependency on time-of-exposure to moist environments of the amount of material erosion in the module intercell zone has been quantified by two methods—directly by EDS analysis, and indirectly by sheet resistivity measurements on fully aluminized back surface modules. In addition, changes in maximum power output, series resistance, and open circuit voltage have been documented. Consequences for fielded modules are discussed.

Acceleration Recorder and Playback Module

NASA Technical Reports Server (NTRS)

Bozeman, Richard J., Jr. (Inventor)

1996-01-01

The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

Acceleration recorder and playback module

NASA Astrophysics Data System (ADS)

Bozeman, Richard J., Jr.

1994-11-01

The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

Acceleration recorder and playback module

NASA Astrophysics Data System (ADS)

Bozeman, Richard J., Jr.

1992-09-01

The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

Acceleration recorder and playback module

NASA Technical Reports Server (NTRS)

Bozeman, Richard J., Jr. (Inventor)

1994-01-01

The present invention is directed to methods and apparatus relating to an accelerometer electrical signal recorder and playback module. The recorder module may be manufactured in lightweight configuration and includes analog memory components to store data. Signal conditioning circuitry is incorporated into the module so that signals may be connected directly from the accelerometer to the recorder module. A battery pack may be included for powering both the module and the accelerometer. Timing circuitry is included to control the time duration within which data is recorded or played back so as to avoid overloading the analog memory components. Multiple accelerometer signal recordings may be taken simultaneously without analog to digital circuits, multiplexing circuitry or software to compensate for the effects of multiplexing the signals.

Vicarious retribution: the role of collective blame in intergroup aggression.

PubMed

Lickel, Brian; Miller, Norman; Stenstrom, Douglas M; Denson, Thomas F; Schmader, Toni

2006-01-01

We provide a new framework for understanding 1 aspect of aggressive conflict between groups, which we refer to as vicarious retribution. Vicarious retribution occurs when a member of a group commits an act of aggression toward the members of an outgroup for an assault or provocation that had no personal consequences for him or her but which did harm a fellow ingroup member. Furthermore, retribution is often directed at outgroup members who, themselves, were not the direct causal agents in the original attack against the person's ingroup. Thus, retribution is vicarious in that neither the agent of retaliation nor the target of retribution were directly involved in the original event that precipitated the intergroup conflict. We describe how ingroup identification, outgroup entitativity, and other variables, such as group power, influence vicarious retribution. We conclude by considering a variety of conflict reduction strategies in light of this new theoretical framework.

Athermal design for the potassium titanyl phosphate electro-optical modulator

NASA Astrophysics Data System (ADS)

Zheng, Guoliang; Xu, Jie; Chen, Lixiang; Wang, Hongcheng; She, Weilong

2007-09-01

An athermal design for the KTP electro-optical modulator is presented. By using the wave coupling theory of linear electro-optic effect and taking account of thermal expansion, the more accurate athermal static phase retardation (ASPR) directions in potassium titanyl phosphate (KTP) are found, and the optimized design for a transverse amplitude modulator at ASPR orientation is obtained. The numerical results show that the modulator with an athermal Soleil-Babinet compensator is of excellent thermal stability, and the acceptable error of the ASPR direction is less than 0.1°.

Characteristics of spectro-temporal modulation frequency selectivity in humans.

PubMed

Oetjen, Arne; Verhey, Jesko L

2017-03-01

There is increasing evidence that the auditory system shows frequency selectivity for spectro-temporal modulations. A recent study of the authors has shown spectro-temporal modulation masking patterns that were in agreement with the hypothesis of spectro-temporal modulation filters in the human auditory system [Oetjen and Verhey (2015). J. Acoust. Soc. Am. 137(2), 714-723]. In the present study, that experimental data and additional data were used to model this spectro-temporal frequency selectivity. The additional data were collected to investigate to what extent the spectro-temporal modulation-frequency selectivity results from a combination of a purely temporal amplitude-modulation filter and a purely spectral amplitude-modulation filter. In contrast to the previous study, thresholds were measured for masker and target modulations with opposite directions, i.e., an upward pointing target modulation and a downward pointing masker modulation. The comparison of this data set with previous corresponding data with the same direction from target and masker modulations indicate that a specific spectro-temporal modulation filter is required to simulate all aspects of spectro-temporal modulation frequency selectivity. A model using a modified Gabor filter with a purely temporal and a purely spectral filter predicts the spectro-temporal modulation masking data.

Sample holder with optical features

DOEpatents

Milas, Mirko; Zhu, Yimei; Rameau, Jonathan David

2013-07-30

A sample holder for holding a sample to be observed for research purposes, particularly in a transmission electron microscope (TEM), generally includes an external alignment part for directing a light beam in a predetermined beam direction, a sample holder body in optical communication with the external alignment part and a sample support member disposed at a distal end of the sample holder body opposite the external alignment part for holding a sample to be analyzed. The sample holder body defines an internal conduit for the light beam and the sample support member includes a light beam positioner for directing the light beam between the sample holder body and the sample held by the sample support member.

Transmission electron microscope sample holder with optical features

DOEpatents

Milas, Mirko [Port Jefferson, NY; Zhu, Yimei [Stony Brook, NY; Rameau, Jonathan David [Coram, NY

2012-03-27

A sample holder for holding a sample to be observed for research purposes, particularly in a transmission electron microscope (TEM), generally includes an external alignment part for directing a light beam in a predetermined beam direction, a sample holder body in optical communication with the external alignment part and a sample support member disposed at a distal end of the sample holder body opposite the external alignment part for holding a sample to be analyzed. The sample holder body defines an internal conduit for the light beam and the sample support member includes a light beam positioner for directing the light beam between the sample holder body and the sample held by the sample support member.

Member Perceptions of the One Health Initiative at a Zoological Institution

PubMed Central

Padda, Hannah; Niedbalski, Amy; Tate, Erin; Deem, Sharon L.

2018-01-01

Zoological institutions play an important role in promoting the goals of the One Health movement. We launched the Institute for Conservation Medicine (ICM) at the Saint Louis Zoo in 2011 to advance the goals of One Health. In 2016, we distributed a survey to Zoo members to evaluate member awareness and understanding of One Health and to provide direction for future communication and actions from the ICM. We hypothesized that Zoo members would be aware of One Health and care about infectious disease issues. Survey results showed Zoo members primarily cared about chronic, non-infectious diseases and their associated economic costs, with participants ranking their top three health issues of concern for humans as nutrition/obesity/diet (49%), costs of health care (48%), and cancer (37%). Zoo members were interested in the roles of zoos in One Health and found them important, but were less aware of the Saint Louis Zoo’s actions that did not directly relate to animal welfare. Only 6% of members had awareness of the term “One Health” and 16% were aware of the term “Conservation Medicine.” These results suggest that zoos may do better to tailor One Health messaging to align with member interests. Messaging and programming from the Saint Louis Zoo will now include the direct benefits to human health that zoos offer, in addition to the ICM’s more ecologically focused activities. This study offered valuable insight into how Zoo members view One Health and may serve as a template to help zoological institutions develop and promote One Health. PMID:29536015

Tumor microenvironment: Modulation by decorin and related molecules harboring leucine-rich tandem motifs.

PubMed

Goldoni, Silvia; Iozzo, Renato V

2008-12-01

Decorin, the prototype member of the small leucine-rich proteoglycans, resides in the tumor microenvironment and affects the biology of various types of cancer by downregulating the activity of several receptors involved in cell growth and survival. Decorin binds to and modulates the signaling of the epidermal growth factor receptor and other members of the ErbB family of receptor tyrosine kinases. It exerts its antitumor activity by a dual mechanism: via inhibition of these key receptors through their physical downregulation coupled with attenuation of their signaling, and by binding to and sequestering TGFbeta. Decorin also modulates the insulin-like growth factor receptor and the low-density lipoprotein receptor-related protein 1, which indirectly affects the TGFbeta receptor pathway. When expressed in tumor xenograft-bearing mice or injected systemically, decorin inhibits both primary tumor growth and metastatic spreading. In this review, we summarize the latest reports on decorin and related molecules that are relevant to cancer and bring forward the idea of decorin as an anticancer therapeutic and possible prognostic marker for patients affected by various types of tumors. We also discuss the role of lumican and LRIG1, a novel cell growth inhibitor homologous to decorin. (c) 2008 Wiley-Liss, Inc.

Strain-induced dimensionality crossover of precursor modulations in Ni2MnGa

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nie, Zhihua; Wang, Yandong; Shang, Shunli

2015-01-01

Precursor modulations often occur in functional materials like magnetic shape memory alloys, ferroelectrics, and superconductors. In this letter, we have revealed the underlying mechanism of the precursor modulations in ferromagnetic shape memory alloys Ni2MnGa by combining synchrotron-based x-ray diffraction experiments and first-principles phonon calculations. We discovered the precursor modulations along [011] direction can be eliminated with [001] uniaxial loading, while the precursor modulations or premartensite can be totally suppressed by hydrostatic pressure condition. The TA2 phonon anomaly is sensitive to stress induced lattice strain, and the entire TA2 branch is stabilized along the directions where precursor modulations are eliminated bymore » external stress. Our discovery bridges precursor modulations and phonon anomalies, and sheds light on the microscopic mechanism of the two-step superelasticity in precursor martensite.« less

Engineering Translational Activators with CRISPR-Cas System.

PubMed

Du, Pei; Miao, Chensi; Lou, Qiuli; Wang, Zefeng; Lou, Chunbo

2016-01-15

RNA parts often serve as critical components in genetic engineering. Here we report a design of translational activators which is composed of an RNA endoribonuclease (Csy4) and two exchangeable RNA modules. Csy4, a member of Cas endoribonuclease, cleaves at a specific recognition site; this cleavage releases a cis-repressive RNA module (crRNA) from the masked ribosome binding site (RBS), which subsequently allows the downstream translation initiation. Unlike small RNA as a translational activator, the endoribonuclease-based activator is able to efficiently unfold the perfect RBS-crRNA pairing. As an exchangeable module, the crRNA-RBS duplex was forwardly and reversely engineered to modulate the dynamic range of translational activity. We further showed that Csy4 and its recognition site, together as a module, can also be replaced by orthogonal endoribonuclease-recognition site homologues. These modularly structured, high-performance translational activators would endow the programming of gene expression in the translation level with higher feasibility.

Staff's attitudes and reactions towards aggressive behaviour of clients with intellectual disabilities: a multi-level study.

PubMed

Knotter, Maartje H; Wissink, Inge B; Moonen, Xavier M H; Stams, Geert-Jan J M; Jansen, Gerard J

2013-05-01

Data were collected from 121 staff members (20 direct support staff teams) on background characteristics of the individual staff members and their teams (gender, age, years of work experience, position and education), the frequency and form of aggression of clients with an intellectual disability (verbal or physical), staff members' attitudes towards aggression, and the types of behavioural interventions they executed (providing personal space and behavioural boundary-setting, restricting freedom and the use of coercive measures). Additionally, client group characteristics (age of clients, type of care and client's level of intellectual disability) were assessed. Multilevel analyses (individual and contextual level) were performed to examine the relations between all studied variables and the behavioural interventions. The results showed that for providing personal space and behavioural boundary-setting as well as for restricting freedom, the proportion of variance explained by the context (staff team and client group characteristics) was three times larger than the proportion of variance explained by individual staff member characteristics. For using coercive measures, the context even accounted for 66% of the variance, whereas only 8% was explained by individual staff member characteristics. A negative attitude towards aggression of the direct support team as a whole proved to be an especially strong predictor of using coercive measures. To diminish the use of coercive measures, interventions should therefore be directed towards influencing the attitude of direct support teams instead of individual staff members. Copyright © 2013 Elsevier Ltd. All rights reserved.

Apparatus for remotely handling components

DOEpatents

Szkrybalo, Gregory A.; Griffin, Donald L.

1994-01-01

The inventive apparatus for remotely handling bar-like components which define a longitudinal direction includes a gripper mechanism for gripping the component including first and second gripper members longitudinally fixedly spaced from each other and oriented parallel to each other in planes transverse to the longitudinal direction. Each gripper member includes a jaw having at least one V-groove with opposing surfaces intersecting at a base and extending radially relative to the longitudinal direction for receiving the component in an open end between the opposing surfaces. The V-grooves on the jaw plate of the first and second gripper members are aligned in the longitudinal direction to support the component in the first and second gripper members. A jaw is rotatably mounted on and a part of each of the first and second gripper members for selectively assuming a retracted mode in which the open end of the V-groove is unobstructed and active mode in which the jaw spans the open end of the V-groove in the first and second gripper members. The jaw has a locking surface for contacting the component in the active mode to secure the component between the locking surface of the jaw and the opposing surfaces of the V-groove. The locking surface has a plurality of stepped portions, each defining a progressively decreasing radial distance between the base of the V-groove and the stepped portion opposing the base to accommodate varying sizes of components.

77 FR 37793 - Airworthiness Directives; the Boeing Company Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-25

... proposed to require installing new structural members in and new tie rod(s) and attach fittings on the left.... ACTION: Final rule. SUMMARY: We are adopting a new airworthiness directive (AD) for certain the Boeing... forward load. This AD requires installing new structural members, tie rod(s), and attach fittings on the...

Effect of supervised, periodized exercise training vs. self-directed training on lean body mass and other fitness variables in health club members.

PubMed

Storer, Thomas W; Dolezal, Brett A; Berenc, Matthew N; Timmins, John E; Cooper, Christopher B

2014-07-01

Conventional wisdom suggests that exercise training with a personal trainer (PTr) is more beneficial for improving health-related fitness than training alone. However, there are no published data that confirm whether fitness club members who exercise with a PTr in the fitness club setting obtain superior results compared with self-directed training. We hypothesized that club members randomized to receive an evidence-based training program would accrue greater improvements in lean body mass (LBM) and other fitness measures than members randomized to self-training. Men, aged 30-44 years, who were members of a single Southern California fitness club were randomized to exercise with a PTr administering a nonlinear periodized training program (TRAINED, N = 17) or to self-directed training (SELF, N = 17); both groups trained 3 days per week for 12 weeks. Lean body mass was determined by dual-energy x-ray absorptiometry. Secondary outcomes included muscle strength 1 repetition maximum (1RM), leg power (vertical jump), and aerobic capacity (V[Combining Dot Above]O2max). TRAINED individuals increased LBM by 1.3 (0.4) kg, mean (SEM) vs. no change in SELF, p = 0.029. Similarly, significantly greater improvements were seen for TRAINED vs. SELF in chest press strength (42 vs. 19%; p = 0.003), peak leg power (6 vs. 0.6%; p < 0.0001), and V[Combining Dot Above]O2max (7 vs. -0.3%; p = 0.01). Leg press strength improved 38 and 25% in TRAINED and SELF, respectively (p = 0.14). We have demonstrated for the first time in a fitness club setting that members whose training is directed by well-qualified PTrs administering evidence-based training regimens achieve significantly greater improvements in LBM and other dimensions of fitness than members who direct their own training.

Receiver bandwidth effects on complex modulation and detection using directly modulated lasers.

PubMed

Yuan, Feng; Che, Di; Shieh, William

2016-05-01

Directly modulated lasers (DMLs) have long been employed for short- and medium-reach optical communications due to their low cost. Recently, a new modulation scheme called complex modulated DMLs has been demonstrated showing a significant optical signal to noise ratio sensitivity enhancement compared with the traditional intensity-only detection scheme. However, chirp-induced optical spectrum broadening is inevitable in complex modulated systems, which may imply a need for high-bandwidth receivers. In this Letter, we study the impact of receiver bandwidth effects on the performance of complex modulation and coherent detection systems based on DMLs. We experimentally demonstrate that such systems exhibit a reasonable tolerance for the reduced receiver bandwidth. For 10 Gbaud 4-level pulse amplitude modulation signals, the required electrical bandwidth is as low as 8.5 and 7.5 GHz for 7% and 20% forward error correction, respectively. Therefore, it is feasible to realize DML-based complex modulated systems using cost-effective receivers with narrow bandwidth.

KSC-07pd0891

NASA Image and Video Library

2007-04-17

KENNEDY SPACE CENTER, FLA. -- In the Space Station Processing Facility, NASA and Japanese Aerospace and Exploration Agency (JAXA) officials welcome the arrival of the Experiment Logistics Module Pressurized Section for the Japanese Experiment Module, or JEM, to the Kennedy Space Center. Seen here at right are JAXA representatives, including Japanese astronaut Takao Doi (center of front row), who is a crew member for mission STS-123 that will deliver the module to the space station. The new International Space Station component arrived at Kennedy March 12 to begin preparations for its future launch on mission STS-123. It will serve as an on-orbit storage area for materials, tools and supplies. It can hold up to eight experiment racks and will attach to the top of another larger pressurized module. Photo credit: NASA/George Shelton

KSC-2009-6510

NASA Image and Video Library

2009-11-20

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, Secondino Brondolo, head of the Space Infrastructure, Thales Alenia Space Italy, addresses the invited guests at a ceremony transferring the ownership of node 3 for the International Space Station from the European Space Agency, or ESA, to NASA. Seated, from left, are Bob Cabana, Kennedy Space Center director; Michael Suffredini, program manager, International Space Station, NASA; William Dowdell, deputy for Operations, International Space Station and Spacecraft Processing, Kennedy; and Bernardo Patti, head of International Space Station, Program Department, ESA. Node 3 is named "Tranquility" after the Sea of Tranquility, the lunar landing site of Apollo 11. The payload for the STS-130 mission, Tranquility is a pressurized module that will provide room for many of the International Space Station's life support systems. The module was built for ESA by Thales Alenia Space in Turin, Italy. Attached to one end of Tranquility is a cupola, a unique work station with six windows on its sides and one on top. The cupola resembles a circular bay window and will provide a vastly improved view of the station's exterior. Just under 10 feet in diameter, the module will accommodate two crew members and portable workstations that can control station and robotic activities. The multi-directional view will allow the crew to monitor spacewalks and docking operations, as well as provide a spectacular view of Earth and other celestial objects. Space shuttle Endeavour's STS-130 mission is targeted to launch Feb. 4, 2010. Photo credit: NASA/Kim Shiflett

Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro

PubMed Central

Smith, Ida M.; Baker, Adam; Christensen, Jeffrey E.; Boekhout, Teun; Frøkiær, Hanne; Arneborg, Nils; Jespersen, Lene

2016-01-01

Interactions between members of the intestinal microbiota and the mucosal immune system can significantly impact human health, and in this context, fungi and food-related yeasts are known to influence intestinal inflammation through direct interactions with specialized immune cells in vivo. The aim of the present study was to characterize the immune modulating properties of the food-related yeast Kluyveromyces marxianus in terms of adaptive immune responses indicating inflammation versus tolerance and to explore the mechanisms behind the observed responses. Benchmarking against a Saccharomyces boulardii strain with probiotic effects documented in clinical trials, we evaluated the ability of K. marxianus to modulate human dendritic cell (DC) function in vitro. Further, we assessed yeast induced DC modulation of naive T cells toward effector responses dominated by secretion of IFNγ and IL-17 versus induction of a Treg response characterized by robust IL-10 secretion. In addition, we blocked relevant DC surface receptors and investigated the stimulating properties of β-glucan containing yeast cell wall extracts. K. marxianus and S. boulardii induced distinct levels of DC cytokine secretion, primarily driven by Dectin-1 recognition of β-glucan components in their cell walls. Upon co-incubation of yeast exposed DCs and naive T cells, S. boulardii induced a potent IFNγ response indicating TH1 mobilization. In contrast, K. marxianus induced a response dominated by Foxp3+ Treg cells, a characteristic that may benefit human health in conditions characterized by excessive inflammation and positions K. marxianus as a strong candidate for further development as a novel yeast probiotic. PMID:27898740

Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro.

PubMed

Smith, Ida M; Baker, Adam; Christensen, Jeffrey E; Boekhout, Teun; Frøkiær, Hanne; Arneborg, Nils; Jespersen, Lene

2016-01-01

Interactions between members of the intestinal microbiota and the mucosal immune system can significantly impact human health, and in this context, fungi and food-related yeasts are known to influence intestinal inflammation through direct interactions with specialized immune cells in vivo. The aim of the present study was to characterize the immune modulating properties of the food-related yeast Kluyveromyces marxianus in terms of adaptive immune responses indicating inflammation versus tolerance and to explore the mechanisms behind the observed responses. Benchmarking against a Saccharomyces boulardii strain with probiotic effects documented in clinical trials, we evaluated the ability of K. marxianus to modulate human dendritic cell (DC) function in vitro. Further, we assessed yeast induced DC modulation of naive T cells toward effector responses dominated by secretion of IFNγ and IL-17 versus induction of a Treg response characterized by robust IL-10 secretion. In addition, we blocked relevant DC surface receptors and investigated the stimulating properties of β-glucan containing yeast cell wall extracts. K. marxianus and S. boulardii induced distinct levels of DC cytokine secretion, primarily driven by Dectin-1 recognition of β-glucan components in their cell walls. Upon co-incubation of yeast exposed DCs and naive T cells, S. boulardii induced a potent IFNγ response indicating TH1 mobilization. In contrast, K. marxianus induced a response dominated by Foxp3+ Treg cells, a characteristic that may benefit human health in conditions characterized by excessive inflammation and positions K. marxianus as a strong candidate for further development as a novel yeast probiotic.

Phosphoproteomic Analysis of Protein Kinase C Signaling in Saccharomyces cerevisiae Reveals Slt2 Mitogen-activated Protein Kinase (MAPK)-dependent Phosphorylation of Eisosome Core Components*

PubMed Central

Mascaraque, Victoria; Hernáez, María Luisa; Jiménez-Sánchez, María; Hansen, Rasmus; Gil, Concha; Martín, Humberto; Cid, Víctor J.; Molina, María

2013-01-01

The cell wall integrity (CWI) pathway of the model organism Saccharomyces cerevisiae has been thoroughly studied as a paradigm of the mitogen-activated protein kinase (MAPK) pathway. It consists of a classic MAPK module comprising the Bck1 MAPK kinase kinase, two redundant MAPK kinases (Mkk1 and Mkk2), and the Slt2 MAPK. This module is activated under a variety of stimuli related to cell wall homeostasis by Pkc1, the only member of the protein kinase C family in budding yeast. Quantitative phosphoproteomics based on stable isotope labeling of amino acids in cell culture is a powerful tool for globally studying protein phosphorylation. Here we report an analysis of the yeast phosphoproteome upon overexpression of a PKC1 hyperactive allele that specifically activates CWI MAPK signaling in the absence of external stimuli. We found 82 phosphopeptides originating from 43 proteins that showed enhanced phosphorylation in these conditions. The MAPK S/T-P target motif was significantly overrepresented in these phosphopeptides. Hyperphosphorylated proteins provide putative novel targets of the Pkc1–cell wall integrity pathway involved in diverse functions such as the control of gene expression, protein synthesis, cytoskeleton maintenance, DNA repair, and metabolism. Remarkably, five components of the plasma-membrane-associated protein complex known as eisosomes were found among the up-regulated proteins. We show here that Pkc1-induced phosphorylation of the eisosome core components Pil1 and Lsp1 was not exerted directly by Pkc1, but involved signaling through the Slt2 MAPK module. PMID:23221999

The secretogranin-II derived peptide secretoneurin modulates electric behavior in the weakly pulse type electric fish, Brachyhypopomus gauderio.

PubMed

Pouso, Paula; Quintana, Laura; López, Gabriela C; Somoza, Gustavo M; Silva, Ana C; Trudeau, Vance L

2015-10-01

Secretoneurin (SN) in the preoptic area and pituitary of mammals and fish has a conserved close association with the vasopressin and oxytocin systems, members of a peptide family that are key in the modulation of sexual and social behaviors. Here we show the presence of SN-immunoreactive cells and projections in the brain of the electric fish, Brachyhypopomus gauderio. Secretoneurin colocalized with vasotocin (AVT) and isotocin in cells and fibers of the preoptic area. In the rostral pars distalis of the pituitary, many cells were both SN and prolactin-positive. In the hindbrain, at the level of the command nucleus of the electric behavior (pacemaker nucleus; PN), some of SN-positive fibers colocalized with AVT. We also explored the potential neuromodulatory role of SN on electric behavior, specifically on the rate of the electric organ discharge (EOD) that signals arousal, dominance and subordinate status. Each EOD is triggered by the command discharge of the PN, ultimately responsible for the basal EOD rate. SN modulated diurnal basal EOD rate in freely swimming fish in a context-dependent manner; determined by the initial value of EOD rate. In brainstem slices, SN partially mimicked the in vivo behavioral effects acting on PN firing rate. Taken together, our results suggest that SN may regulate electric behavior, and that its effect on EOD rate may be explained by direct action of SN at the PN level through either neuroendocrine and/or endocrine mechanisms. Copyright © 2015 Elsevier Inc. All rights reserved.

Medical student knowledge regarding radiology before and after a radiological anatomy module: implications for vertical integration and self-directed learning.

PubMed

Murphy, Kevin P; Crush, Lee; O'Malley, Eoin; Daly, Fergus E; O'Tuathaigh, Colm M P; O'Connor, Owen J; Cryan, John F; Maher, Michael M

2014-10-01

To examine the impact that anatomy-focused radiology teaching has on non-examined knowledge regarding radiation safety and radiology as a specialty. First-year undergraduate medical students completed surveys prior to and after undertaking the first-year anatomy programme that incorporates radiological anatomy. Students were asked opinions on preferred learning methodology and tested on understanding of radiology as a specialty and radiation safety. Pre-module and post-module response rates were 93 % (157/168) and 85 % (136/160), respectively. Pre-module and post-module, self-directed learning (SDL) ranked eighth (of 11) for preferred gross-anatomy teaching formats. Correct responses regarding radiologist/radiographer roles varied from 28-94 % on 16 questions with 4/16 significantly improving post-module. Identification of modalities that utilise radiation significantly improved for five of eight modalities post-module but knowledge regarding relative amount of modality-specific radiation use was variable pre-module and post-module. SDL is not favoured as an anatomy teaching method. Exposure of students to a radiological anatomy module delivered by senior clinical radiologists improved basic knowledge regarding ionising radiation use, but there was no improvement in knowledge regarding radiation exposure relative per modality. A possible explanation is that students recall knowledge imparted in didactic lectures but do little reading around the subject when the content is not examined. • Self-directed learning is not favoured as a gross anatomy teaching format amongst medical students. • An imaging anatomy-focused module improved basic knowledge regarding ionising radiation use. • Detailed knowledge of modality-specific radiation exposure remained suboptimal post-module. • Knowledge of roles within a clinical radiology department showed little change post-module.

Kinase cascades and ligand-directed signaling at the kappa opioid receptor.

PubMed

Bruchas, Michael R; Chavkin, Charles

2010-06-01

The dynorphin/kappa opioid receptor (KOR) system has been implicated as a critical component of the stress response. Stress-induced activation of dynorphin-KOR is well known to produce analgesia, and more recently, it has been implicated as a mediator of stress-induced responses including anxiety, depression, and reinstatement of drug seeking. Drugs selectively targeting specific KOR signaling pathways may prove potentially useful as therapeutic treatments for mood and addiction disorders. KOR is a member of the seven transmembrane spanning (7TM) G-protein coupled receptor (GPCR) superfamily. KOR activation of pertussis toxin-sensitive G proteins leads to Galphai/o inhibition of adenylyl cyclase production of cAMP and releases Gbetagamma, which modulates the conductances of Ca(+2) and K(+) channels. In addition, KOR agonists activate kinase cascades including G-protein coupled Receptor Kinases (GRK) and members of the mitogen-activated protein kinase (MAPK) family: ERK1/2, p38 and JNK. Recent pharmacological data suggests that GPCRs exist as dynamic, multi-conformational protein complexes that can be directed by specific ligands towards distinct signaling pathways. Ligand-induced conformations of KOR that evoke beta-arrestin-dependent p38 MAPK activation result in aversion; whereas ligand-induced conformations that activate JNK without activating arrestin produce long-lasting inactivation of KOR signaling. In this review, we discuss the current status of KOR signal transduction research and the data that support two novel hypotheses: (1) KOR selective partial agonists that do not efficiently activate p38 MAPK may be useful analgesics without producing the dysphoric or hallucinogenic effects of selective, highly efficacious KOR agonists and (2) KOR antagonists that do not activate JNK may be effective short-acting drugs that may promote stress-resilience.

A novel heat shock protein alpha 8 (Hspa8) molecular network mediating responses to stress- and ethanol-related behaviors.

PubMed

Urquhart, Kyle R; Zhao, Yinghong; Baker, Jessica A; Lu, Ye; Yan, Lei; Cook, Melloni N; Jones, Byron C; Hamre, Kristin M; Lu, Lu

2016-04-01

Genetic differences mediate individual differences in susceptibility and responses to stress and ethanol, although, the specific molecular pathways that control these responses are not fully understood. Heat shock protein alpha 8 (Hspa8) is a molecular chaperone and member of the heat shock protein family that plays an integral role in the stress response and that has been implicated as an ethanol-responsive gene. Therefore, we assessed its role in mediating responses to stress and ethanol across varying genetic backgrounds. The hippocampus is an important mediator of these responses, and thus, was examined in the BXD family of mice in this study. We conducted bioinformatic analyses to dissect genetic factors modulating Hspa8 expression, identify downstream targets of Hspa8, and examined its role. Hspa8 is trans-regulated by a gene or genes on chromosome 14 and is part of a molecular network that regulates stress- and ethanol-related behaviors. To determine additional components of this network, we identified direct or indirect targets of Hspa8 and show that these genes, as predicted, participate in processes such as protein folding and organic substance metabolic processes. Two phenotypes that map to the Hspa8 locus are anxiety-related and numerous other anxiety- and/or ethanol-related behaviors significantly correlate with Hspa8 expression. To more directly assay this relationship, we examined differences in gene expression following exposure to stress or alcohol and showed treatment-related differential expression of Hspa8 and a subset of the members of its network. Our findings suggest that Hspa8 plays a vital role in genetic differences in responses to stress and ethanol and their interactions.

A note on elongations of summable QTAG-modules.

PubMed

Mehdi, Alveera; Sikander, Fahad; Naji, Sabah A R K

2013-01-01

A right module M over an associative ring with unity is a QTAG-module if every finitely generated submodule of any homomorphic image of M is a direct sum of uniserial modules. In this paper we find a suitable condition under which a special ω-elongation of a summable QTAG-module by a ( ω +k)-projective QTAG-module is also a summable QTAG-module.

A Note on Elongations of Summable QTAG-Modules

PubMed Central

Mehdi, Alveera; Naji, Sabah A. R. K.

2013-01-01

A right module M over an associative ring with unity is a QTAG-module if every finitely generated submodule of any homomorphic image of M is a direct sum of uniserial modules. In this paper we find a suitable condition under which a special ω-elongation of a summable QTAG-module by a (ω+k)-projective QTAG-module is also a summable QTAG-module. PMID:24459429

Jet-controlled freeze valve for use in a glass melter

DOEpatents

Routt, Kenneth R.

1986-09-02

A drain valve for use in a furnace for the melting of thermoplastic material. The furnace includes a drain cavity formed in its bottom for withdrawing a flow of thermoplastic material. The drain valve includes a flow member which include a flow tube having an inlet and outlet for the material, and coaxially disposed concentric tubular members defining annuli surrounding the flow tube. The tubular members include heating and cooling means for the flow tube. The flow member is adapted to fit in mating relationship in the drain cavity. A freeze valve member is disposed adjacent the outlet of the flow member. The freeze valve member includes heating means and has a plurality of air jets adapted to direct streams of pressurized air at the outlet to control the flow of thermoplastic material through the flow members. The drain valve can also be used in a furnace of glass melting that includes a drain cavity for withdrawing molten glass from the furnace. The drain valve includes a flow tube member having an inlet and outlet, and having heating and cooling means. The tube member is adapted to fit in mating relationship with the drain cavity. A freeze valve member is disposed at the outlet of the flow tube member. The freeze valve member includes heating means and has a plurality of air jets adapted to direct a stream of pressurized air at the outlet to control the flow of glass through the flow tube member.

Jet-controlled freeze valve for use in a glass melter

DOEpatents

Routt, Kenneth R.

1986-01-01

A drain valve for use in a furnace for the melting of thermoplastic material. The furnace includes a drain cavity formed in its bottom for withdrawing a flow of thermoplastic material. The drain valve includes a flow member which include a flow tube having an inlet and outlet for the material, and coaxially disposed concentric tubular members defining annuli surrounding the flow tube. The tubular members include heating and cooling means for the flow tube. The flow member is adapted to fit in mating relationship in the drain cavity. A freeze valve member is disposed adjacent the outlet of the flow member. The freeze valve member includes heating means and has a plurality of air jets adapted to direct streams of pressurized air at the outlet to control the flow of thermoplastic material through the flow members. The drain valve can also be used in a furnace of glass melting that includes a drain cavity for withdrawing molten glass from the furnace. The drain valve includes a flow tube member having an inlet and outlet, and having heating and cooling means. The tube member is adapted to fit in mating relationship with the drain cavity. A freeze valve member is disposed at the outlet of the flow tube member. The freeze valve member includes heating means and has a plurality of air jets adapted to direct a stream of pressurized air at the outlet to control the flow of glass through the flow tube member.

KSC-01pp0872

NASA Image and Video Library

2001-03-19

KENNEDY SPACE CENTER, FLA. -- Members of the STS-104 crew look over equipment inside the equipment lock component of the Joint Airlock Module. At left is Mission Specialist Janet L. Kavandi, and at right Pilot Charles O. Hobaugh. The crew is at KSC to take part in Crew Equipment Interface Test activities. The mission will carry the Joint Airlock Module to the International Space Station. The U.S.-made module will allow astronauts and cosmonauts in residence on the Station to perform future spacewalks without the presence of a Space Shuttle. The module, which also comprises a crew lock, will be connected to the starboard (right) side of Node 1 Unity. Atlantis will also carry oxygen and nitrogen storage tanks, vital to operation of the Joint Airlock, on a Spacelab Logistics Double Pallet in the payload bay. The tanks, to be installed on the perimeter of the Joint Module during the mission’s spacewalks, will support future spacewalk operations and experiments plus augment the resupply system for the Station’s Service Module

KSC-01pp0871

NASA Image and Video Library

2001-03-19

KENNEDY SPACE CENTER, FLA. -- Members of the STS-104 crew look over equipment inside the equipment lock component of the Joint Airlock Module. At left is Mission Specialist Janet L. Kavandi, and at right Pilot Charles O. Hobaugh. The crew is at KSC to take part in Crew Equipment Interface Test activities. The mission will carry the Joint Airlock Module to the International Space Station. The U.S.-made module will allow astronauts and cosmonauts in residence on the Station to perform future spacewalks without the presence of a Space Shuttle. The module, which also comprises a crew lock, will be connected to the starboard (right) side of Node 1 Unity. Atlantis will also carry oxygen and nitrogen storage tanks, vital to operation of the Joint Airlock, on a Spacelab Logistics Double Pallet in the payload bay. The tanks, to be installed on the perimeter of the Joint Module during the mission’s spacewalks, will support future spacewalk operations and experiments plus augment the resupply system for the Station’s Service Module

MS Ivins floats through U.S. Laboratory / Destiny module

NASA Image and Video Library

2001-02-11

STS98-E-5161 (11 February 2001) --- Astronaut Marsha S. Ivins, STS-98 mission specialist, floats into the newly attached Destiny laboratory onboard the International Space Station (ISS). After the Destiny hatch was opened early in the day, members of both crews went to work quickly inside the new module, activating air systems, fire extinguishers, alarm systems, computers and internal communications. The crews also took some photos and continued equipment transfers from the shuttle to the station. The scene was taken with a digital still camera.

Kuipers closes hatch

NASA Image and Video Library

2012-03-24

ISS030-E-173931 (24 March 2012) --- European Space Agency astronaut Andre Kuipers, Expedition 30 flight engineer, closes a hatch in the International Space Station as crew members prepare to move to the appropriate Soyuz vehicles, due to the possibility that space debris could pass close to the station. Burbank, Shkaplerov and Ivanishin sheltered in the Soyuz TMA-22 spacecraft attached to the Poisk Mini-Research Module 2 (MRM2) while Kononenko, Kuipers and Pettit took to the Soyuz TMA-03M docked to the Rassvet Mini-Research Module 1 (MRM-1).

KSC-08pd1842

NASA Image and Video Library

2008-06-26

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center, STS-126 crew members check out the interior of the multi-purpose logistics module that will fly on the mission. Shuttle crews frequently visit Kennedy to get hands-on experience, called a crew equipment interface test, with hardware and equipment for their missions. On STS-126, Endeavour will deliver a multi-purpose logistics module to the International Space Station. Launch is targeted for Nov. 10. Photo credit: NASA/Kim Shiflett

Candid view of Astronaut Lucid in the Spektr module

NASA Image and Video Library

1996-09-16

NM22-427-012 (16-26 Sept. 1996) --- During off-duty time on the Spektr Module aboard the Earth-orbiting Mir Space Station, astronaut Shannon W. Lucid, cosmonaut guest researcher, retrieves a book from her personal library. Lucid, dropped off in March by the STS-76 crew members, was nearing the end of 188 consecutive days in space before returning to Earth with the STS-79 crew. She worked with a total of five cosmonauts at various times during that stay.

Analyzing Commodity Council Development and Implementation: The Air Force Furnishings Commodity Council

DTIC Science & Technology

2011-12-01

all production carried on by one big firm? … First, as a firm gets larger, there may be decreasing returns to the entrepreneur function, that is, the...the entrepreneur fails to place the factors of production in the uses where their value is greatest, that is, fails to make the best use of the...three one-week classroom modules. UT coordinated with AFFCC to ensure team members received the module training at a specific point during the strategy

STS-135 crew during AEM (Animal Enclosure Module) training

NASA Image and Video Library

2011-03-25

JSC2011-E-029131 (25 March 2011) --- STS-135 crew members participate in an Animal Enclosure Module (AEM) training session in the Jake Garn Simulation and Training Facility at NASA's Johnson Space Center. Pictured on the right (front to back) are NASA astronauts Chris Ferguson, commander; Sandy Magnus and Rex Walheim, both mission specialists; along with Doug Hurley (left foreground), pilot. STS-135 is planned to be the final mission of the space shuttle program. Photo credit: NASA or National Aeronautics and Space Administration

STS-135 crew during AEM (Animal Enclosure Module) training

NASA Image and Video Library

2011-03-25

JSC2011-E-029132 (25 March 2011) --- STS-135 crew members participate in an Animal Enclosure Module (AEM) training session in the Jake Garn Simulation and Training Facility at NASA's Johnson Space Center. Pictured from the left (facing camera) are NASA astronauts Rex Walheim and Sandy Magnus, both mission specialists; and Chris Ferguson, commander; along with Doug Hurley (right foreground), pilot. STS-135 is planned to be the final mission of the space shuttle program. Photo credit: NASA or National Aeronautics and Space Administration

KSC-00pp1842

NASA Image and Video Library

2000-12-05

KENNEDY SPACE CENTER, Fla. -- STS-107 crew members take part in In-Flight Maintenance training for their mission. Under the watchful eyes of SPACEHAB trainer David Butler (left), Payload Specialist Ilan Ramon of Israel (center) and Mission Specialist David M. Brown (right) check equipment and paperwork inside the SPACEHAB Double Module. Research mission STS-107, scheduled to launch July 19, 2001, will carry the SPACEHAB Double Module in its first flight into space and a broad collection of experiments ranging from material science to life science

KSC00pp1842

NASA Image and Video Library

2000-12-05

KENNEDY SPACE CENTER, Fla. -- STS-107 crew members take part in In-Flight Maintenance training for their mission. Under the watchful eyes of SPACEHAB trainer David Butler (left), Payload Specialist Ilan Ramon of Israel (center) and Mission Specialist David M. Brown (right) check equipment and paperwork inside the SPACEHAB Double Module. Research mission STS-107, scheduled to launch July 19, 2001, will carry the SPACEHAB Double Module in its first flight into space and a broad collection of experiments ranging from material science to life science

KSC00pp1841

NASA Image and Video Library

2000-12-05

KENNEDY SPACE CENTER, Fla. -- Members of the STS-107 crew take part in In-Flight Maintenance training for their mission. One of the payload elements on the mission is the SPACEHAB Double Module in its first research flight into space. Working with equipment inside the SPACEHAB Double Module are (left to right) Commander Rick D. Husband, Payload Specialist Ilan Ramon of Israel and Pilot William C. "Willie" McCool , while SPACEHAB trainer David Butler (standing) looks on. Research mission STS-107 is scheduled to launch July 19, 2001

KSC-00pp1834

NASA Image and Video Library

2000-12-06

KENNEDY SPACE CENTER, Fla. -- Members of the STS-107 crew take part in In-Flight Maintenance training for their mission. One of the payload elements on the mission is the SPACEHAB Double Module in its first research flight into space. Working with equipment inside the SPACEHAB Double Module are (left to right) SPACEHAB trainer David Butler, Pilot William C. “Willie” McCool, Commander Rick D. Husband, and Payload Specialist Ilan Ramon, from Israel. Research mission STS-107 is scheduled to launch July 19, 2001

KSC-00pp1835

NASA Image and Video Library

2000-12-06

KENNEDY SPACE CENTER, Fla. -- Members of the STS-107 crew take part in In-Flight Maintenance training for their mission. One of the payload elements on the mission is the SPACEHAB Double Module in its first research flight into space. Working with equipment inside the SPACEHAB Double Module are (front) Payload Specialist Ilan Ramon (left), astronaut from Israel, and Commander Rick D. Husband; (back) Mission Specialist David Brown, SPACEHAB trainer David Butler, and Pilot William C. “Willie” McCool. Research mission STS-107 is scheduled to launch July 19, 2001

KSC00pp1835

NASA Image and Video Library

2000-12-06

KENNEDY SPACE CENTER, Fla. -- Members of the STS-107 crew take part in In-Flight Maintenance training for their mission. One of the payload elements on the mission is the SPACEHAB Double Module in its first research flight into space. Working with equipment inside the SPACEHAB Double Module are (front) Payload Specialist Ilan Ramon (left), astronaut from Israel, and Commander Rick D. Husband; (back) Mission Specialist David Brown, SPACEHAB trainer David Butler, and Pilot William C. “Willie” McCool. Research mission STS-107 is scheduled to launch July 19, 2001

KSC-00pp1841

NASA Image and Video Library

2000-12-05

KENNEDY SPACE CENTER, Fla. -- Members of the STS-107 crew take part in In-Flight Maintenance training for their mission. One of the payload elements on the mission is the SPACEHAB Double Module in its first research flight into space. Working with equipment inside the SPACEHAB Double Module are (left to right) Commander Rick D. Husband, Payload Specialist Ilan Ramon of Israel and Pilot William C. "Willie" McCool , while SPACEHAB trainer David Butler (standing) looks on. Research mission STS-107 is scheduled to launch July 19, 2001

KSC00pp1834

NASA Image and Video Library

2000-12-06

KENNEDY SPACE CENTER, Fla. -- Members of the STS-107 crew take part in In-Flight Maintenance training for their mission. One of the payload elements on the mission is the SPACEHAB Double Module in its first research flight into space. Working with equipment inside the SPACEHAB Double Module are (left to right) SPACEHAB trainer David Butler, Pilot William C. “Willie” McCool, Commander Rick D. Husband, and Payload Specialist Ilan Ramon, from Israel. Research mission STS-107 is scheduled to launch July 19, 2001

STS-47 crew poses for official onboard (in space) portrait in SLJ module

NASA Image and Video Library

1992-09-20

STS047-12-002 (12 - 20 Sept 1992) --- The crew members assemble for their traditional in-flight portrait in this 35mm frame photographed in the Science Module aboard the Earth-orbiting Space Shuttle Endeavour. Left to right (front) are N. Jan Davis, Mark C. Lee and Mamoru Mohri; and (rear) Curtis L. Brown, Jr., Jerome (Jay) Apt, Robert L. Gibson and Mae C. Jemison. The seven spent eight days in space in support of the Spacelab-J mission.

Lexan Linear Shaped Charge Holder with Magnets and Backing Plate

NASA Technical Reports Server (NTRS)

Maples, Matthew W.; Dutton, Maureen L.; Hacker, Scott C.; Dean, Richard J.; Kidd, Nicholas; Long, Chris; Hicks, Robert C.

2013-01-01

A method was developed for cutting a fabric structural member in an inflatable module, without damaging the internal structure of the module, using linear shaped charge. Lexan and magnets are used in a charge holder to precisely position the linear shaped charge over the desired cut area. Two types of charge holders have been designed, each with its own backing plate. One holder cuts fabric straps in the vertical configuration, and the other charge holder cuts fabric straps in the horizontal configuration.

Solar Cell Modules With Improved Backskin

DOEpatents

Chevrefils, Andre; Grigore, Daniel Gheorghe

2001-01-23

The present invention relates to gas turbines and more particularly to a device for controlling the flow of cooling air through a flowpath in a turbine blade. The device can be inserted in the inlet opening of the blade flowpath and be retained therein. The device comprises a plug member for adjusting the flow of cooling air through the flowpath. The plug member comprises a retaining portion for retaining the plug member at the inlet opening of the flowpath and a blocking portion inserted within the flowpath for reducing the cross-sectional area of the inlet opening. Such a device is inexpensive and can be easily inserted in the inlet opening of a blade flowpath and retained therein.

Spatio-temporal regulation of connexin43 phosphorylation and gap junction dynamics.

PubMed

Solan, Joell L; Lampe, Paul D

2018-01-01

Gap junctions are specialized membrane domains containing tens to thousands of intercellular channels. These channels permit exchange of small molecules (<1000Da) including ions, amino acids, nucleotides, metabolites and secondary messengers (e.g., calcium, glucose, cAMP, cGMP, IP 3 ) between cells. The common reductionist view of these structures is that they are composed entirely of integral membrane proteins encoded by the 21 member connexin human gene family. However, it is clear that the normal physiological function of this structure requires interaction and regulation by a variety of proteins, especially kinases. Phosphorylation is capable of directly modulating connexin channel function but the most dramatic effects on gap junction activity occur via the organization of the gap junction structures themselves. This is a direct result of the short half-life of the primary gap junction protein, connexin, which requires them to be constantly assembled, remodeled and turned over. The biological consequences of this remodeling are well illustrated during cardiac ischemia, a process wherein gap junctions are disassembled and remodeled resulting in arrhythmia and ultimately heart failure. This article is part of a Special Issue entitled: Gap Junction Proteins edited by Jean Claude Herve. Copyright © 2017 Elsevier B.V. All rights reserved.

Under a neighbour's influence: public information affects stress hormones and behaviour of a songbird

PubMed Central

Cornelius, Jamie M.; Breuner, Creagh W.; Hahn, Thomas P.

2010-01-01

Socially acquired information improves the accuracy and efficiency of environmental assessments and can increase fitness. Public information may be especially useful during unpredictable food conditions, or for species that depend on resources made less predictable by human disturbance. However, the physiological mechanisms by which direct foraging assessments and public information are integrated to affect behaviour remain largely unknown. We tested for potential effects of public information on the behavioural and hormonal response to food reduction by manipulating the social environment of captive red crossbills (Loxia curvirostra). Red crossbills are irruptive migrants that are considered sensitive to changes in food availability and use public information in decision making. Here, we show that public information can attenuate or intensify the release of glucocorticoids (i.e. stress hormones) during food shortage in red crossbills. The observed modulation of corticosterone may therefore be a physiological mechanism linking public information, direct environmental assessments and behavioural change. This mechanism would not only allow for public information to affect individual behaviour, but might also facilitate group decision making by bringing group members into more similar physiological states. The results further suggest that stressors affecting entire populations may be magnified in individual physiology through social interactions. PMID:20356895

Maximum likelihood sequence estimation for optical complex direct modulation.

PubMed

Che, Di; Yuan, Feng; Shieh, William

2017-04-17

Semiconductor lasers are versatile optical transmitters in nature. Through the direct modulation (DM), the intensity modulation is realized by the linear mapping between the injection current and the light power, while various angle modulations are enabled by the frequency chirp. Limited by the direct detection, DM lasers used to be exploited only as 1-D (intensity or angle) transmitters by suppressing or simply ignoring the other modulation. Nevertheless, through the digital coherent detection, simultaneous intensity and angle modulations (namely, 2-D complex DM, CDM) can be realized by a single laser diode. The crucial technique of CDM is the joint demodulation of intensity and differential phase with the maximum likelihood sequence estimation (MLSE), supported by a closed-form discrete signal approximation of frequency chirp to characterize the MLSE transition probability. This paper proposes a statistical method for the transition probability to significantly enhance the accuracy of the chirp model. Using the statistical estimation, we demonstrate the first single-channel 100-Gb/s PAM-4 transmission over 1600-km fiber with only 10G-class DM lasers.

Dynamic tailoring of surface plasmon polaritons through incident angle modulation.

PubMed

Qiu, Peizhen; Zhang, Dawei; Jing, Ming; Lu, Taiguo; Yu, Binbin; Zhan, Qiwen; Zhuang, Songlin

2018-04-16

Dynamic tailoring of the propagating surface plasmon polaritons (SPPs) through incident angle modulation is proposed and numerically demonstrated. The generation and tailoring mechanism of the SPPs are discussed. The relationship formula between the incident angle and the generated SPP wave vector direction is theoretically derived. The correctness of the formula is verified with three different approaches using finite difference time domain method. Using this formula, the generated SPP wave vector direction can be precisely modulated by changing the incident angle. The precise modulation results of two dimensional Bessel-like SPP beam and SPP bottle beam array are given. The results can deepen the understanding of the generation and modulation mechanism of the SPPs.

The Community Surface Dynamics Modeling System: Experiences on Building a Collaborative Modeling Platform

NASA Astrophysics Data System (ADS)

Overeem, I.; Hutton, E.; Kettner, A.; Peckham, S. D.; Syvitski, J. P.

2012-12-01

The Community Surface Dynamics Modeling System - CSDMS- develops a software platform with shared and coupled modules for modeling earth surface processes as a community resource. The framework allows prediction of water, sediment and nutrient transport through the landscape and seacape. The underlying paradigm is that the Earth surface we live on is a dynamic system; topography changes with seasons, with landslides and earthquakes, with erosion and deposition. The Earth Surface changes due to storms and floods, and important boundaries, like the coast, are ever-moving features. CSDMS sets out to make better predictions of these changes. Earth surface process modeling bridges the terrestrial, coastal and marine domains and requires understanding of the system over a range of time scales, which inherently needs interdisciplinarity. Members of CSDMS (~830 in July 2012) are largely from academic institutions (˜75%), followed by federal agencies (˜17%), and oil and gas companies (˜5%). Members and governmental bodies meet once annually and rely additionally on web-based information for communication. As an organization that relies on volunteer participation, CSDMS faces challenges to scientific collaboration. Encouraging volunteerism among its members to provide and adapt metadata and model code to be sufficiently standardized for coupling is crucial to building an integrated community modeling system. We here present CSDMS strategies aimed at providing the appropriate technical tools and cyberinfrastructure to support a variety of user types, ranging from advanced to novice modelers. Application of these advances in science is key, both into the educational realm and for managers and decision-makers. We discuss some of the implemented ideas to further organizational transparency and user engagement in small-scale governance, such as advanced trackers and voting systems for model development prioritization through the CSDMS wiki. We analyzed data on community contributions and novice user engagement and evaluate the effectiveness of CSDMS' strategies toward these two challenges over the first 5 years based on member and user data, surveys, computing logs and web log analysis. Analysis shows that sponsored member participation in annual meetings (˜30%) is relatively high. Direct CSDMS governance relies on ˜4% of members. About 15% of members contributed code and metadata, and 18% use the common supercomputing resources. Technological development and documentation lie predominantly in hands of funded members, and a small number of others (˜3% together). Potential new users are trained in clinics and courses, and on a one-to-one basis with quantified positive effects on self-efficacy and recruitment of new advanced developers.

A side-by-side comparison of CPV module and system performance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Muller, Matthew; Marion, Bill; Kurtz, Sarah

A side-by-side comparison is made between concentrator photovoltaic module and system direct current aperture efficiency data with a focus on quantifying system performance losses. The individual losses measured/calculated, when combined, are in good agreement with the total loss seen between the module and the system. Results indicate that for the given test period, the largest individual loss of 3.7% relative is due to the baseline performance difference between the individual module and the average for the 200 modules in the system. A basic empirical model is derived based on module spectral performance data and the tabulated losses between the modulemore » and the system. The model predicts instantaneous system direct current aperture efficiency with a root mean square error of 2.3% relative.« less

The AC photovoltaic module is here!

NASA Astrophysics Data System (ADS)

Strong, Steven J.; Wohlgemuth, John H.; Wills, Robert H.

1997-02-01

This paper describes the design, development, and performance results of a large-area photovoltaic module whose electrical output is ac power suitable for direct connection to the utility grid. The large-area ac PV module features a dedicated, integrally mounted, high-efficiency dc-to-ac power inverter with a nominal output of 250 watts (STC) at 120 Vac, 60 H, that is fully compatible with utility power. The module's output is connected directly to the building's conventional ac distribution system without need for any dc wiring, string combiners, dc ground-fault protection or additional power-conditioning equipment. With its advantages, the ac photovoltaic module promises to become a universal building block for use in all utility-interactive PV systems. This paper discusses AC Module design aspects and utility interface issues (including islanding).

Direction-Finding Measurements of Heliospheric 2-3 kHz Radio Emissions

NASA Technical Reports Server (NTRS)

Gurnett, Donald A.

1998-01-01

Using data from the Voyager 1 plasma wave instrument, a series of direction-finding measurements is presented for the intense 1992-93 heliospheric 2- to 3-kHz radio emission event, and several weaker events extending into 1994. Direction-finding measurements can only be obtained during roll maneuvers, which are performed about once every three months. Two parameters can be determined from the roll-induced intensity modulation, the azimuthal direction of arrival (measured around the roll axis), and the modulation index (the peak-to-peak amplitude divided by the peak amplitude). Measurements were made at two frequencies, 1.78 and 3.11 kHz. No roll modulation was observed at 1.78 kHz, which is consistent with an isotropic source at this frequency. In most cases an easily measurable roll modulation was detectable at 3.11 kHz. Although the azimuth angles have considerable scatter, the directions of arrival at 3.11 kHz can be organized into three groups, each of which appears to be associated with a separate upward drifting feature in the radio emission spectrum. The first group, which is associated with the main 1992-93 event, is consistent with a source located near the nose of the heliosphere. The remaining two groups, which occur after the main 1992-93 event, have azimuth angles well away from the nose of the heliosphere. The modulation indexes vary over a large range, from 0.06 to 0.61, with no obvious trend. Although the variations in the directions of arrival and modulation indicies appear to reflect changes in the position and angular size of the source, it is also possible that they could be caused by refraction or scattering due to density structures in the solar wind.

Tensiometer, drive probe for use with environmental testing equipment, and methods of inserting environmental testing equipment into a sample

DOEpatents

Hubbell, Joel M.; Sisson, James B.

2005-07-26

A method of inserting a tensiometer into a sample, comprises providing a drive probe configured to be engaged by direct push equipment; supporting a porous member from the drive probe; and driving the drive probe into the sample using a cone penetrometer. A tensiometer comprises a drive probe configured to be engaged by direct push equipment or a cone penetrometer; a porous member supported by the drive probe; and a pressure sensor in pressure sensing relation to the porous member.

On the benefit of DMT modulation in nonlinear VLC systems.

PubMed

Qian, Hua; Cai, Sunzeng; Yao, Saijie; Zhou, Ting; Yang, Yang; Wang, Xudong

2015-02-09

In a visible light communication (VLC) system, the nonlinear characteristic of the light emitting diode (LED) in transmitter is a limiting factor of system performance. Modern modulation signals with large peak-to-power-ratio (PAPR) suffers uneven distortion. The nonlinear response directly impacts the intensity modulation and direct detection VLC system with pulse-amplitude modulation (PAM). The amplitude of the PAM signal is distorted unevenly and large signal is vulnerable to noise. Orthogonal linear transformations, such as discrete multi-tone (DMT) modulation, can spread the nonlinear effects evenly to each data symbol, thus perform better than PAM signals. In this paper, we provide theoretical analysis on the benefit of DMT modulation in nonlinear VLC system. We show that the DMT modulation is a better choice than the PAM modulation for the VLC system as the DMT modulation is more robust against nonlinearity. We also show that the post-distortion nonlinear elimination method, which is applied at the receiver, can be a reliable solution to the nonlinear VLC system. Simulation results show that the post-distortion greatly improves the system performance for the DMT modulation.

Rational Tuning of Visual Cycle Modulator Pharmacodynamics

PubMed Central

Kiser, Philip D.; Zhang, Jianye; Badiee, Mohsen; Kinoshita, Junzo; Peachey, Neal S.; Tochtrop, Gregory P.

2017-01-01

Modulators of the visual cycle have been developed for treatment of various retinal disorders. These agents were designed to inhibit retinoid isomerase [retinal pigment epithelium-specific 65 kDa protein (RPE65)], the rate-limiting enzyme of the visual cycle, based on the idea that attenuation of visual pigment regeneration could reduce formation of toxic retinal conjugates. Of these agents, certain ones that contain primary amine groups can also reversibly form retinaldehyde Schiff base adducts, which contributes to their retinal protective activity. Direct inhibition of RPE65 as a therapeutic strategy is complicated by adverse effects resulting from slowed chromophore regeneration, whereas effective retinal sequestration can require high drug doses with potential off-target effects. We hypothesized that the RPE65-emixustat crystal structure could help guide the design of retinaldehyde-sequestering agents with varying degrees of RPE65 inhibitory activity. We found that addition of an isopropyl group to the central phenyl ring of emixustat and related compounds resulted in agents effectively lacking in vitro retinoid isomerase inhibitory activity, whereas substitution of the terminal 6-membered ring with branched moieties capable of stronger RPE65 interaction potentiated inhibition. The isopropyl derivative series produced discernible visual cycle suppression in vivo, albeit much less potently than compounds with a high affinity for the RPE65 active site. These agents were distributed into the retina and formed Schiff base adducts with retinaldehyde. Except for one compound [3-amino-1-(3-isopropyl-5-((2,6,6-trimethylcyclohex-1-en-1-yl)methoxy)phenyl)propan-1-ol (MB-007)], these agents conferred protection against retinal phototoxicity, suggesting that both direct RPE65 inhibition and retinal sequestration are mechanisms of potential therapeutic relevance. PMID:28476927

Measuring coalescing massive binary black holes with gravitational waves: The impact of spin-induced precession

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lang, Ryan N.; Hughes, Scott A.

The coalescence of massive black holes generates gravitational waves (GWs) that will be measurable by space-based detectors such as LISA to large redshifts. The spins of a binary's black holes have an important impact on its waveform. Specifically, geodetic and gravitomagnetic effects cause the spins to precess; this precession then modulates the waveform, adding periodic structure which encodes useful information about the binary's members. Following pioneering work by Vecchio, we examine the impact upon GW measurements of including these precession-induced modulations in the waveform model. We find that the additional periodicity due to spin precession breaks degeneracies among certain parameters,more » greatly improving the accuracy with which they may be measured. In particular, mass measurements are improved tremendously, by one to several orders of magnitude. Localization of the source on the sky is also improved, though not as much--low redshift systems can be localized to an ellipse which is roughly 10-a fewx10 arcminutes in the long direction and a factor of 2 smaller in the short direction. Though not a drastic improvement relative to analyses which neglect spin precession, even modest gains in source localization will greatly facilitate searches for electromagnetic counterparts to GW events. Determination of distance to the source is likewise improved: We find that relative error in measured luminosity distance is commonly {approx}0.1%-0.4% at z{approx}1. Finally, with the inclusion of precession, we find that the magnitude of the spins themselves can typically be determined for low redshift systems with an accuracy of about 0.1%-10%, depending on the spin value, allowing accurate surveys of mass and spin evolution over cosmic time.« less

Activated RhoA Is a Positive Feedback Regulator of the Lbc Family of Rho Guanine Nucleotide Exchange Factor Proteins*

PubMed Central

Medina, Frank; Carter, Angela M.; Dada, Olugbenga; Gutowski, Stephen; Hadas, Jana; Chen, Zhe; Sternweis, Paul C.

2013-01-01

The monomeric Rho GTPases are essential for cellular regulation including cell architecture and movement. A direct mechanism for hormonal regulation of the RhoA-type GTPases is their modulation by the G12 and G13 proteins via RH (RGS homology) containing RhoGEFs. In addition to the interaction of the G protein α subunits with the RH domain, activated RhoA also binds to the pleckstrin homology (PH) domain of PDZRhoGEF. The latter interaction is now extended to all seven members of the homologous Lbc family of RhoGEFs which includes the RH-RhoGEFs. This is evinced by direct measurements of binding or through effects on selected signaling pathways in cells. Overexpression of these PH domains alone can block RhoA-dependent signaling in cells to various extents. Whereas activated RhoA does not modulate the intrinsic activity of the RhoGEFs, activated RhoA associated with phospholipid vesicles can facilitate increased activity of soluble RhoGEFs on vesicle-delimited substrate (RhoA-GDP). This demonstrates feasibility of the hypothesis that binding of activated RhoA to the PH domains acts as a positive feedback mechanism. This is supported by cellular studies in which mutation of this binding site on PH strongly attenuates the stimulation of RhoA observed by overexpression of five of the RhoGEF DH-PH domains. This mutation is even more dramatic in the context of full-length p115RhoGEF. The utilization of this mechanism by multiple RhoGEFs suggests that this regulatory paradigm may be a common feature in the broader family of RhoGEFs. PMID:23493395

Discrete and structurally unique proteins (tāpirins) mediate attachment of extremely thermophilic Caldicellulosiruptor species to cellulose.

PubMed

Blumer-Schuette, Sara E; Alahuhta, Markus; Conway, Jonathan M; Lee, Laura L; Zurawski, Jeffrey V; Giannone, Richard J; Hettich, Robert L; Lunin, Vladimir V; Himmel, Michael E; Kelly, Robert M

2015-04-24

A variety of catalytic and noncatalytic protein domains are deployed by select microorganisms to deconstruct lignocellulose. These extracellular proteins are used to attach to, modify, and hydrolyze the complex polysaccharides present in plant cell walls. Cellulolytic enzymes, often containing carbohydrate-binding modules, are key to this process; however, these enzymes are not solely responsible for attachment. Few mechanisms of attachment have been discovered among bacteria that do not form large polypeptide structures, called cellulosomes, to deconstruct biomass. In this study, bioinformatics and proteomics analyses identified unique, discrete, hypothetical proteins ("tāpirins," origin from Māori: to join), not directly associated with cellulases, that mediate attachment to cellulose by species in the noncellulosomal, extremely thermophilic bacterial genus Caldicellulosiruptor. Two tāpirin genes are located directly downstream of a type IV pilus operon in strongly cellulolytic members of the genus, whereas homologs are absent from the weakly cellulolytic Caldicellulosiruptor species. Based on their amino acid sequence, tāpirins are specific to these extreme thermophiles. Tāpirins are also unusual in that they share no detectable protein domain signatures with known polysaccharide-binding proteins. Adsorption isotherm and trans vivo analyses demonstrated the carbohydrate-binding module-like affinity of the tāpirins for cellulose. Crystallization of a cellulose-binding truncation from one tāpirin indicated that these proteins form a long β-helix core with a shielded hydrophobic face. Furthermore, they are structurally unique and define a new class of polysaccharide adhesins. Strongly cellulolytic Caldicellulosiruptor species employ tāpirins to complement substrate-binding proteins from the ATP-binding cassette transporters and multidomain extracellular and S-layer-associated glycoside hydrolases to process the carbohydrate content of lignocellulose. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.