Science.gov

Sample records for discrete membrane-trafficking steps

  1. Membrane trafficking in neuronal maintenance and degeneration.

    PubMed

    Wang, Dong; Chan, Chih-Chiang; Cherry, Smita; Hiesinger, P Robin

    2013-08-01

    Defects in membrane trafficking and degradation are hallmarks of most, and maybe all, neurodegenerative disorders. Such defects typically result in the accumulation of undegraded proteins due to aberrant endosomal sorting, lysosomal degradation, or autophagy. The genetic or environmental cause of a specific disease may directly affect these membrane trafficking processes. Alternatively, changes in intracellular sorting and degradation can occur as cellular responses of degenerating neurons to unrelated primary defects such as insoluble protein aggregates or other neurotoxic insults. Importantly, altered membrane trafficking may contribute to the pathogenesis or indeed protect the neuron. The observation of dramatic changes to membrane trafficking thus comes with the challenging need to distinguish pathological from protective alterations. Here, we will review our current knowledge about the protective and destructive roles of membrane trafficking in neuronal maintenance and degeneration. In particular, we will first focus on the question of what type of membrane trafficking keeps healthy neurons alive in the first place. Next, we will discuss what alterations of membrane trafficking are known to occur in Alzheimer's disease and other tauopathies, Parkinson's disease, polyQ diseases, peripheral neuropathies, and lysosomal storage disorders. Combining the maintenance and degeneration viewpoints may yield insight into how to distinguish when membrane trafficking functions protectively or contributes to degeneration.

  2. Diacylglycerol kinases in membrane trafficking

    PubMed Central

    Xie, Shuwei; Naslavsky, Naava; Caplan, Steve

    2015-01-01

    Diacylglycerol kinases (DGKs) belong to a family of cytosolic kinases that regulate the phosphorylation of diacylglycerol (DAG), converting it into phosphatidic acid (PA). There are 10 known mammalian DGK isoforms, each with a different tissue distribution and substrate specificity. These differences allow regulation of cellular responses by fine-tuning the delicate balance of cellular DAG and PA. DGK isoforms are best characterized as mediators of signal transduction and immune function. However, since recent studies reveal that DAG and PA are also involved in the regulation of endocytic trafficking, it is therefore anticipated that DGKs also plays an important role in membrane trafficking. In this review, we summarize the literature discussing the role of DGK isoforms at different stages of endocytic trafficking, including endocytosis, exocytosis, endocytic recycling, and transport from/to the Golgi apparatus. Overall, these studies contribute to our understanding of the involvement of PA and DAG in endocytic trafficking, an area of research that is drawing increasing attention in recent years. PMID:27057419

  3. Chemical Genetic Dissection of Membrane Trafficking.

    PubMed

    Norambuena, Lorena; Tejos, Ricardo

    2017-04-28

    The plant endomembrane system is an extensively connected functional unit for exchanging material between compartments. Secretory and endocytic pathways allow dynamic trafficking of proteins, lipids, and other molecules, regulating a myriad of biological processes. Chemical genetics-the use of compounds to perturb biological processes in a fast, tunable, and transient manner-provides elegant tools for investigating this system. Here, we review how chemical genetics has helped to elucidate different aspects of membrane trafficking. We discuss different strategies for uncovering the modes of action of such compounds and their use in unraveling membrane trafficking regulators. We also discuss how the bioactive chemicals that are currently used as probes to interrogate endomembrane trafficking were discovered and analyze the results regarding membrane trafficking and pathway crosstalk. The integration of different expertises and the rational implementation of chemical genetic strategies will improve the identification of molecular mechanisms that drive intracellular trafficking and our understanding of how trafficking interfaces with plant physiology and development.

  4. Cellular membrane trafficking of mesoporous silica nanoparticles

    SciTech Connect

    Fang, I-Ju

    2012-01-01

    This dissertation mainly focuses on the investigation of the cellular membrane trafficking of mesoporous silica nanoparticles. We are interested in the study of endocytosis and exocytosis behaviors of mesoporous silica nanoparticles with desired surface functionality. The relationship between mesoporous silica nanoparticles and membrane trafficking of cells, either cancerous cells or normal cells was examined. Since mesoporous silica nanoparticles were applied in many drug delivery cases, the endocytotic efficiency of mesoporous silica nanoparticles needs to be investigated in more details in order to design the cellular drug delivery system in the controlled way. It is well known that cells can engulf some molecules outside of the cells through a receptor-ligand associated endocytosis. We are interested to determine if those biomolecules binding to cell surface receptors can be utilized on mesoporous silica nanoparticle materials to improve the uptake efficiency or govern the mechanism of endocytosis of mesoporous silica nanoparticles. Arginine-glycine-aspartate (RGD) is a small peptide recognized by cell integrin receptors and it was reported that avidin internalization was highly promoted by tumor lectin. Both RGD and avidin were linked to the surface of mesoporous silica nanoparticle materials to investigate the effect of receptor-associated biomolecule on cellular endocytosis efficiency. The effect of ligand types, ligand conformation and ligand density were discussed in Chapter 2 and 3. Furthermore, the exocytosis of mesoporous silica nanoparticles is very attractive for biological applications. The cellular protein sequestration study of mesoporous silica nanoparticles was examined for further information of the intracellular pathway of endocytosed mesoporous silica nanoparticle materials. The surface functionality of mesoporous silica nanoparticle materials demonstrated selectivity among the materials and cancer and normal cell lines. We aimed to determine

  5. Inositol lipid phosphatases in membrane trafficking and human disease.

    PubMed

    Billcliff, Peter G; Lowe, Martin

    2014-07-15

    The specific interaction of phosphoinositides with proteins is critical for a plethora of cellular processes, including cytoskeleton remodelling, mitogenic signalling, ion channel regulation and membrane traffic. The spatiotemporal restriction of different phosphoinositide species helps to define compartments within the cell, and this is particularly important for membrane trafficking within both the secretory and endocytic pathways. Phosphoinositide homoeostasis is tightly regulated by a large number of inositol kinases and phosphatases, which respectively phosphorylate and dephosphorylate distinct phosphoinositide species. Many of these enzymes have been implicated in regulating membrane trafficking and, accordingly, their dysregulation has been linked to a number of human diseases. In the present review, we focus on the inositol phosphatases, concentrating on their roles in membrane trafficking and the human diseases with which they have been associated.

  6. Cell biology symposium: Membrane trafficking and signal transduction

    USDA-ARS?s Scientific Manuscript database

    In general, membrane trafficking is a broad group of processes where proteins and other large molecules are distributed throughout the cell as well as adjacent extracellular spaces. Whereas signal transduction is a process where signals are transmitted through a series of chemical or molecular event...

  7. Roles of membrane trafficking in plant cell wall dynamics

    PubMed Central

    Ebine, Kazuo; Ueda, Takashi

    2015-01-01

    The cell wall is one of the characteristic components of plant cells. The cell wall composition differs among cell types and is modified in response to various environmental conditions. To properly generate and modify the cell wall, many proteins are transported to the plasma membrane or extracellular space through membrane trafficking, which is one of the key protein transport mechanisms in eukaryotic cells. Given the diverse composition and functions of the cell wall in plants, the transport of the cell wall components and proteins that are involved in cell wall-related events could be specialized for each cell type, i.e., the machinery for cell wall biogenesis, modification, and maintenance could be transported via different trafficking pathways. In this review, we summarize the recent progress in the current understanding of the roles and mechanisms of membrane trafficking in plant cells and focus on the biogenesis and regulation of the cell wall. PMID:26539200

  8. Cadherin tales: Regulation of cadherin function by endocytic membrane trafficking.

    PubMed

    Cadwell, Chantel M; Su, Wenji; Kowalczyk, Andrew P

    2016-12-01

    Cadherins are the primary adhesion molecules in adherens junctions and desmosomes and play essential roles in embryonic development. Although significant progress has been made in understanding cadherin structure and function, we lack a clear vision of how cells confer plasticity upon adhesive junctions to allow for cellular rearrangements during development, wound healing and metastasis. Endocytic membrane trafficking has emerged as a fundamental mechanism by which cells confer a dynamic state to adhesive junctions. Recent studies indicate that the juxtamembrane domain of classical cadherins contains multiple endocytic motifs, or "switches," that can be used by cellular membrane trafficking machinery to regulate adhesion. The cadherin-binding protein p120-catenin (p120) appears to be the master regulator of access to these switches, thereby controlling cadherin endocytosis and turnover. This review focuses on p120 and other cadherin-binding proteins, ubiquitin ligases, and growth factors as key modulators of cadherin membrane trafficking. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  9. Sphingosine and Sphingosine Kinase 1 Involvement in Endocytic Membrane Trafficking.

    PubMed

    Lima, Santiago; Milstien, Sheldon; Spiegel, Sarah

    2017-02-24

    The balance between cholesterol and sphingolipids within the plasma membrane has long been implicated in endocytic membrane trafficking. However, in contrast to cholesterol functions, little is still known about the roles of sphingolipids and their metabolites. Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Here we show that sphingosine and sphingosine-like SphK1 inhibitors induced rapid and massive formation of vesicles in diverse cell types that accumulated as dilated late endosomes. However, much smaller vesicles were formed in SphK1-deficient cells. Moreover, inhibition or deletion of SphK1 prolonged the lifetime of sphingosine-induced vesicles. Perturbing the plasma membrane cholesterol/sphingomyelin balance abrogated vesicle formation. This massive endosomal influx was accompanied by dramatic recruitment of the intracellular SphK1 and Bin/Amphiphysin/Rvs domain-containing proteins endophilin-A2 and endophilin-B1 to enlarged endosomes and formation of highly dynamic filamentous networks containing endophilin-B1 and SphK1. Together, our results highlight the importance of sphingosine and its conversion to sphingosine-1-phosphate by SphK1 in endocytic membrane trafficking. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  10. An evolutionary balance: conservation vs innovation in ciliate membrane trafficking.

    PubMed

    Guerrier, Sabrice; Plattner, Helmut; Richardson, Elisabeth; Dacks, Joel B; Turkewitz, Aaron P

    2017-01-01

    As most of eukaryotic diversity lies in single-celled protists, they represent unique opportunities to ask questions about the balance of conservation and innovation in cell biological features. Among free-living protists the ciliates offer ease of culturing, a rich array of experimental approaches, and versatile molecular tools, particularly in Tetrahymena thermophila and Paramecium tetraurelia. These attributes have been exploited by researchers to analyze a wealth of cellular structures in these large and complex cells. This mini-review focuses on 3 aspects of ciliate membrane dynamics, all linked with endolysosomal trafficking. First is nutrition based on phagocytosis and maturation of food vacuoles. Secondly, we discuss regulated exocytosis from vesicles that have features of both dense core secretory granules but also lysosome-related organelles. The third topic is the targeting, breakdown and resorption of parental nuclei in mating partners. For all 3 phenomena, it is clear that elements of the canonical membrane-trafficking system have been retained and in some cases repurposed. In addition, there is evidence that recently evolved, lineage-specific proteins provide determinants in these pathways.

  11. Purinergic receptor stimulation increases membrane trafficking in brown adipocytes

    PubMed Central

    1996-01-01

    Stimulation of brown adipocytes by their sympathetic innervation plays a major role in body energy homeostasis by regulating the energy- wasting activity of the tissue. The norepinephrine released by sympathetic activity acts on adrenergic receptors to activate a variety of metabolic and membrane responses. Since sympathetic stimulation may also release vesicular ATP, we tested brown fat cells for ATP responses. We find that micromolar concentrations of extracellular ATP initiates profound changes in the membrane trafficking of brown adipocytes. ATP elicited substantial increases in total cell membrane capacitance, averaging approximately 30% over basal levels and occurring on a time scale of seconds to minutes. The membrane capacitance increase showed an agonist sensitivity of 2-methylthio-ATP > or = ATP > ADP > > adenosine, consistent with mediation by a P2r type purinergic receptor. Membrane capacitance increases were not seen when cytosolic calcium was increased by adrenergic stimulation, and capacitance responses to ATP were similar in the presence and absence of extracellular calcium. These results indicate that increases in cytosolic calcium alone do not mediate the membrane response to ATP. Photometric assessment of surface-accessible membrane using the dye FM1- 43 showed that ATP caused an approximate doubling of the amount of membrane actively trafficking with the cell surface. The discrepancy in the magnitudes of the capacitance and fluorescence changes suggests that ATP both activates exocytosis and alters other aspects of membrane handling. These findings suggest that secretion, mobilization of membrane transporters, and/or surface membrane expression of receptors may be regulated in brown adipocytes by P2r purinergic receptor activity. PMID:8923265

  12. Missing Pieces of an Ancient Puzzle: Evolution of the Eukaryotic Membrane-Trafficking System

    PubMed Central

    Schlacht, Alexander; Herman, Emily K.; Klute, Mary J.; Field, Mark C.; Dacks, Joel B.

    2014-01-01

    The membrane-trafficking system underpins cellular trafficking of material in eukaryotes and its evolution would have been a watershed in eukaryogenesis. Evolutionary cell biological studies have been unraveling the history of proteins responsible for vesicle transport and organelle identity revealing both highly conserved components and lineage-specific innovations. Recently, endomembrane components with a broad, but patchy, distribution have been observed as well, pieces that are missing from our cell biological and evolutionary models of membrane trafficking. These data together allow for new insights into the history and forces that shape the evolution of this critical cell biological system. PMID:25274701

  13. Missing pieces of an ancient puzzle: evolution of the eukaryotic membrane-trafficking system.

    PubMed

    Schlacht, Alexander; Herman, Emily K; Klute, Mary J; Field, Mark C; Dacks, Joel B

    2014-10-01

    The membrane-trafficking system underpins cellular trafficking of material in eukaryotes and its evolution would have been a watershed in eukaryogenesis. Evolutionary cell biological studies have been unraveling the history of proteins responsible for vesicle transport and organelle identity revealing both highly conserved components and lineage-specific innovations. Recently, endomembrane components with a broad, but patchy, distribution have been observed as well, pieces that are missing from our cell biological and evolutionary models of membrane trafficking. These data together allow for new insights into the history and forces that shape the evolution of this critical cell biological system. Copyright © 2014 Cold Spring Harbor Laboratory Press; all rights reserved.

  14. Recent Advances in Chlamydia Subversion of Host Cytoskeletal and Membrane Trafficking Pathways

    PubMed Central

    Scidmore, Marci A.

    2011-01-01

    Chlamydia species are obligate intracellular bacteria that cause sexually transmitted disease, ocular infections and atypical pneumonia. This review highlights recent advances describing the mechanisms by which Chlamydia subvert host cytoskeleton and membrane trafficking pathways to create a replication competent niche. PMID:21334451

  15. Endogenously Tagged Rab Proteins: A Resource to Study Membrane Trafficking in Drosophila

    PubMed Central

    Dunst, Sebastian; Kazimiers, Tom; von Zadow, Felix; Jambor, Helena; Sagner, Andreas; Brankatschk, Beate; Mahmoud, Ali; Spannl, Stephanie; Tomancak, Pavel; Eaton, Suzanne; Brankatschk, Marko

    2015-01-01

    Summary Membrane trafficking is key to the cell biological mechanisms underlying development. Rab GTPases control specific membrane compartments, from core secretory and endocytic machinery to less-well-understood compartments. We tagged all 27 Drosophila Rabs with YFPMYC at their endogenous chromosomal loci, determined their expression and subcellular localization in six tissues comprising 23 cell types, and provide this data in an annotated, searchable image database. We demonstrate the utility of these lines for controlled knockdown and show that similar subcellular localization can predict redundant functions. We exploit this comprehensive resource to ask whether a common Rab compartment architecture underlies epithelial polarity. Strikingly, no single arrangement of Rabs characterizes the five epithelia we examine. Rather, epithelia flexibly polarize Rab distribution, producing membrane trafficking architectures that are tissue- and stage-specific. Thus, the core machinery responsible for epithelial polarization is unlikely to rely on polarized positioning of specific Rab compartments. PMID:25942626

  16. Lgl1 activation of rab10 promotes axonal membrane trafficking underlying neuronal polarization.

    PubMed

    Wang, Tong; Liu, Yang; Xu, Xiao-Hui; Deng, Cai-Yun; Wu, Kong-Yan; Zhu, Ji; Fu, Xiu-Qing; He, Miao; Luo, Zhen-Ge

    2011-09-13

    Directed membrane trafficking is believed to be crucial for axon development during neuronal morphogenesis. However, the underlying mechanisms are poorly understood. Here, we report a role of Lgl1, the mammalian homolog of Drosophila tumor suppressor Lethal giant larvae, in controlling membrane trafficking underlying axonal growth. We find that Lgl1 is associated with plasmalemmal precursor vesicles and enriched in developing axons. Lgl1 upregulation promoted axonal growth, whereas downregulation attenuated it as well as directional membrane insertion. Interestingly, Lgl1 interacted with and activated Rab10, a small GTPase that mediates membrane protein trafficking, by releasing GDP dissociation inhibitor (GDI) from Rab10. Furthermore, Rab10 lies downstream of Lgl1 in axon development and directional membrane insertion. Finally, both Lgl1 and Rab10 are required for neocortical neuronal polarization in vivo. Thus, the Lgl1 regulation of Rab10 stimulates the trafficking of membrane precursor vesicles, whose fusion with the plasmalemma is crucial for axonal growth.

  17. Regulation of cytokinesis by membrane trafficking involving small GTPases and the ESCRT machinery.

    PubMed

    Nakayama, Kazuhisa

    2016-01-01

    During cell division, cells undergo membrane remodeling to achieve changes in their size and shape. In addition, cell division entails local delivery and retrieval of membranes and specific proteins as well as remodeling of cytoskeletons, in particular, upon cytokinetic abscission. Accumulating lines of evidence highlight that endocytic membrane removal from and subsequent membrane delivery to the plasma membrane are crucial for the changes in cell size and shape, and that trafficking of vesicles carrying specific proteins to the abscission site participate in local remodeling of membranes and cytoskeletons. Furthermore, the endosomal sorting complex required for transport (ESCRT) machinery has been shown to play crucial roles in cytokinetic abscission. Here, the author briefly overviews membrane-trafficking events early in cell division, and subsequently focus on regulation and functional significance of membrane trafficking involving Rab11 and Arf6 small GTPases in late cytokinesis phases and assembly of the ESCRT machinery in cytokinetic abscission.

  18. Neuron membrane trafficking and protein kinases involved in autism and ADHD.

    PubMed

    Kitagishi, Yasuko; Minami, Akari; Nakanishi, Atsuko; Ogura, Yasunori; Matsuda, Satoru

    2015-01-30

    A brain-enriched multi-domain scaffolding protein, neurobeachin has been identified as a candidate gene for autism patients. Mutations in the synaptic adhesion protein cell adhesion molecule 1 (CADM1) are also associated with autism spectrum disorder, a neurodevelopmental disorder of uncertain molecular origin. Potential roles of neurobeachin and CADM1 have been suggested to a function of vesicle transport in endosomal trafficking. It seems that protein kinase B (AKT) and cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) have key roles in the neuron membrane trafficking involved in the pathogenesis of autism. Attention deficit hyperactivity disorder (ADHD) is documented to dopaminergic insufficiencies, which is attributed to synaptic dysfunction of dopamine transporter (DAT). AKT is also essential for the DAT cell-surface redistribution. In the present paper, we summarize and discuss the importance of several protein kinases that regulate the membrane trafficking involved in autism and ADHD, suggesting new targets for therapeutic intervention.

  19. Endogenously tagged rab proteins: a resource to study membrane trafficking in Drosophila.

    PubMed

    Dunst, Sebastian; Kazimiers, Tom; von Zadow, Felix; Jambor, Helena; Sagner, Andreas; Brankatschk, Beate; Mahmoud, Ali; Spannl, Stephanie; Tomancak, Pavel; Eaton, Suzanne; Brankatschk, Marko

    2015-05-04

    Membrane trafficking is key to the cell biological mechanisms underlying development. Rab GTPases control specific membrane compartments, from core secretory and endocytic machinery to less-well-understood compartments. We tagged all 27 Drosophila Rabs with YFP(MYC) at their endogenous chromosomal loci, determined their expression and subcellular localization in six tissues comprising 23 cell types, and provide this data in an annotated, searchable image database. We demonstrate the utility of these lines for controlled knockdown and show that similar subcellular localization can predict redundant functions. We exploit this comprehensive resource to ask whether a common Rab compartment architecture underlies epithelial polarity. Strikingly, no single arrangement of Rabs characterizes the five epithelia we examine. Rather, epithelia flexibly polarize Rab distribution, producing membrane trafficking architectures that are tissue- and stage-specific. Thus, the core machinery responsible for epithelial polarization is unlikely to rely on polarized positioning of specific Rab compartments.

  20. Harnessing membrane trafficking to promote cancer spreading and invasion: The case of RAB2A.

    PubMed

    Kajiho, Hiroaki; Kajiho, Yuko; Scita, Giorgio

    2017-01-06

    How cancer disseminates and metastasizes remains an outstanding open question. Emerging evidence indicates that membrane trafficking is frequently harnessed by tumors of epithelial origin to acquire a mesenchymal program of invasiveness. However, the critical molecular hubs used by cancer cells this context have only began to be elucidated. Here, we discussed the results of a recent phenotypic screening that led to the identification of the small GTPase RAB2A, not previously involved in cancer dissemination, as pivotal for the acquisition of pericellular proteolysis, cell dissemination and distant metastatic spreading of human breast cancer. At the cellular levels, RAB2A controls both canonical polarized Golgi-to-Plasma membrane trafficking of the junctional protein E-cadherin, and post-endocytic trafficking of the membrane-bound metalloprotease, MT1-MMP. This finding reveals an unexpected plasticity in the control of diverse trafficking routes exerted by RAB2A through canonical (Golgi stacking) and non-canonical (late endosome recycling) functional interactions, contributing to break established membrane trafficking dogma on the rigorous molecular distinction between polarized Golgi and post endocytic routes. Finally, they suggest that epithelial cancers may specifically select for those molecules that enable them to control multiple trafficking routes, in turn essential for the regulation of activities necessary for acquisition of mesenchymal traits.

  1. Distinct Human and Mouse Membrane Trafficking Systems for Sweet Taste Receptors T1r2 and T1r3

    PubMed Central

    Shimizu, Madoka; Goto, Masao; Kawai, Takayuki; Yamashita, Atsuko; Kusakabe, Yuko

    2014-01-01

    The sweet taste receptors T1r2 and T1r3 are included in the T1r taste receptor family that belongs to class C of the G protein-coupled receptors. Heterodimerization of T1r2 and T1r3 is required for the perception of sweet substances, but little is known about the mechanisms underlying this heterodimerization, including membrane trafficking. We developed tagged mouse T1r2 and T1r3, and human T1R2 and T1R3 and evaluated membrane trafficking in human embryonic kidney 293 (HEK293) cells. We found that human T1R3 surface expression was only observed when human T1R3 was coexpressed with human T1R2, whereas mouse T1r3 was expressed without mouse T1r2 expression. A domain-swapped chimera and truncated human T1R3 mutant showed that the Venus flytrap module and cysteine-rich domain (CRD) of human T1R3 contain a region related to the inhibition of human T1R3 membrane trafficking and coordinated regulation of human T1R3 membrane trafficking. We also found that the Venus flytrap module of both human T1R2 and T1R3 are needed for membrane trafficking, suggesting that the coexpression of human T1R2 and T1R3 is required for this event. These results suggest that the Venus flytrap module and CRD receive taste substances and play roles in membrane trafficking of human T1R2 and T1R3. These features are different from those of mouse receptors, indicating that human T1R2 and T1R3 are likely to have a novel membrane trafficking system. PMID:25029362

  2. Distinct human and mouse membrane trafficking systems for sweet taste receptors T1r2 and T1r3.

    PubMed

    Shimizu, Madoka; Goto, Masao; Kawai, Takayuki; Yamashita, Atsuko; Kusakabe, Yuko

    2014-01-01

    The sweet taste receptors T1r2 and T1r3 are included in the T1r taste receptor family that belongs to class C of the G protein-coupled receptors. Heterodimerization of T1r2 and T1r3 is required for the perception of sweet substances, but little is known about the mechanisms underlying this heterodimerization, including membrane trafficking. We developed tagged mouse T1r2 and T1r3, and human T1R2 and T1R3 and evaluated membrane trafficking in human embryonic kidney 293 (HEK293) cells. We found that human T1R3 surface expression was only observed when human T1R3 was coexpressed with human T1R2, whereas mouse T1r3 was expressed without mouse T1r2 expression. A domain-swapped chimera and truncated human T1R3 mutant showed that the Venus flytrap module and cysteine-rich domain (CRD) of human T1R3 contain a region related to the inhibition of human T1R3 membrane trafficking and coordinated regulation of human T1R3 membrane trafficking. We also found that the Venus flytrap module of both human T1R2 and T1R3 are needed for membrane trafficking, suggesting that the coexpression of human T1R2 and T1R3 is required for this event. These results suggest that the Venus flytrap module and CRD receive taste substances and play roles in membrane trafficking of human T1R2 and T1R3. These features are different from those of mouse receptors, indicating that human T1R2 and T1R3 are likely to have a novel membrane trafficking system.

  3. Stability with large step sizes for multistep discretizations of stiff ordinary differential equations

    NASA Technical Reports Server (NTRS)

    Majda, George

    1986-01-01

    One-leg and multistep discretizations of variable-coefficient linear systems of ODEs having both slow and fast time scales are investigated analytically. The stability properties of these discretizations are obtained independent of ODE stiffness and compared. The results of numerical computations are presented in tables, and it is shown that for large step sizes the stability of one-leg methods is better than that of the corresponding linear multistep methods.

  4. Stability with large step sizes for multistep discretizations of stiff ordinary differential equations

    NASA Technical Reports Server (NTRS)

    Majda, George

    1986-01-01

    One-leg and multistep discretizations of variable-coefficient linear systems of ODEs having both slow and fast time scales are investigated analytically. The stability properties of these discretizations are obtained independent of ODE stiffness and compared. The results of numerical computations are presented in tables, and it is shown that for large step sizes the stability of one-leg methods is better than that of the corresponding linear multistep methods.

  5. Organelle Size Scaling of the Budding Yeast Vacuole Is Tuned by Membrane Trafficking Rates

    PubMed Central

    Chan, Yee-Hung Mark; Marshall, Wallace F.

    2014-01-01

    Organelles serve as biochemical reactors in the cell, and often display characteristic scaling trends with cell size, suggesting mechanisms that coordinate their sizes. In this study, we measure the vacuole-cell size scaling trends in budding yeast using optical microscopy and a novel, to our knowledge, image analysis algorithm. Vacuole volume and surface area both show characteristic scaling trends with respect to cell size that are consistent among different strains. Rapamycin treatment was found to increase vacuole-cell size scaling trends for both volume and surface area. Unexpectedly, these increases did not depend on macroautophagy, as similar increases in vacuole size were observed in the autophagy deficient mutants atg1Δ and atg5Δ. Rather, rapamycin appears to act on vacuole size by inhibiting retrograde membrane trafficking, as the atg18Δ mutant, which is defective in retrograde trafficking, shows similar vacuole size scaling to rapamycin-treated cells and is itself insensitive to rapamycin treatment. Disruption of anterograde membrane trafficking in the apl5Δ mutant leads to complementary changes in vacuole size scaling. These quantitative results lead to a simple model for vacuole size scaling based on proportionality between cell growth rates and vacuole growth rates. PMID:24806931

  6. The large discretization step method for time-dependent partial differential equations

    NASA Technical Reports Server (NTRS)

    Haras, Zigo; Taasan, Shlomo

    1995-01-01

    A new method for the acceleration of linear and nonlinear time dependent calculations is presented. It is based on the Large Discretization Step (LDS) approximation, defined in this work, which employs an extended system of low accuracy schemes to approximate a high accuracy discrete approximation to a time dependent differential operator. Error bounds on such approximations are derived. These approximations are efficiently implemented in the LDS methods for linear and nonlinear hyperbolic equations, presented here. In these algorithms the high and low accuracy schemes are interpreted as the same discretization of a time dependent operator on fine and coarse grids, respectively. Thus, a system of correction terms and corresponding equations are derived and solved on the coarse grid to yield the fine grid accuracy. These terms are initialized by visiting the fine grid once in many coarse grid time steps. The resulting methods are very general, simple to implement and may be used to accelerate many existing time marching schemes.

  7. A Step-by-Step Framework on Discrete Events Simulation in Emergency Department; A Systematic Review

    PubMed Central

    Dehghani, Mahsa; Moftian, Nazila; Rezaei-Hachesu, Peyman; Samad-Soltani, Taha

    2017-01-01

    Objective: To systematically review the current literature of simulation in healthcare including the structured steps in the emergency healthcare sector by proposing a framework for simulation in the emergency department. Methods: For the purpose of collecting the data, PubMed and ACM databases were used between the years 2003 and 2013. The inclusion criteria were to select English-written articles available in full text with the closest objectives from among a total of 54 articles retrieved from the databases. Subsequently, 11 articles were selected for further analysis. Results: The studies focused on the reduction of waiting time and patient stay, optimization of resources allocation, creation of crisis and maximum demand scenarios, identification of overcrowding bottlenecks, investigation of the impact of other systems on the existing system, and improvement of the system operations and functions. Subsequently, 10 simulation steps were derived from the relevant studies after an expert’s evaluation. Conclusion: The 10-steps approach proposed on the basis of the selected studies provides simulation and planning specialists with a structured method for both analyzing problems and choosing best-case scenarios. Moreover, following this framework systematically enables the development of design processes as well as software implementation of simulation problems. PMID:28507994

  8. Evolutionary analysis of the ENTH/ANTH/VHS protein superfamily reveals a coevolution between membrane trafficking and metabolism.

    PubMed

    De Craene, Johan-Owen; Ripp, Raymond; Lecompte, Odile; Thompson, Julie D; Poch, Olivier; Friant, Sylvie

    2012-07-02

    Membrane trafficking involves the complex regulation of proteins and lipids intracellular localization and is required for metabolic uptake, cell growth and development. Different trafficking pathways passing through the endosomes are coordinated by the ENTH/ANTH/VHS adaptor protein superfamily. The endosomes are crucial for eukaryotes since the acquisition of the endomembrane system was a central process in eukaryogenesis. Our in silico analysis of this ENTH/ANTH/VHS superfamily, consisting of proteins gathered from 84 complete genomes representative of the different eukaryotic taxa, revealed that genomic distribution of this superfamily allows to discriminate Fungi and Metazoa from Plantae and Protists. Next, in a four way genome wide comparison, we showed that this discriminative feature is observed not only for other membrane trafficking effectors, but also for proteins involved in metabolism and in cytokinesis, suggesting that metabolism, cytokinesis and intracellular trafficking pathways co-evolved. Moreover, some of the proteins identified were implicated in multiple functions, in either trafficking and metabolism or trafficking and cytokinesis, suggesting that membrane trafficking is central to this co-evolution process. Our study suggests that membrane trafficking and compartmentalization were not only key features for the emergence of eukaryotic cells but also drove the separation of the eukaryotes in the different taxa.

  9. Src regulates membrane trafficking of the Kv3.1b channel.

    PubMed

    Bae, Seong Han; Kim, Dong Hyun; Shin, Seok Kyo; Choi, Jin Sung; Park, Kang-Sik

    2014-01-03

    The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expression of the Kv3.1b channel with active Src induced a remarkable decrease in the pool of Kv3.1b at the cell surface. Moreover, the co-expression of active Src results in a significant decrease in the peak current densities of the Kv3.1b channel, and a substantial alteration in the voltage dependence of its steady-state inactivation. Taken together, these results indicate that Src kinase may play an important role in regulating membrane trafficking of Kv3.1b channels.

  10. A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival*

    PubMed Central

    Beckley, Janel R.; Chen, Jun-Song; Yang, Yanling; Peng, Junmin; Gould, Kathleen L.

    2015-01-01

    Deubiquitinating enzymes (DUBs), cysteine or metallo- proteases that cleave ubiquitin chains or protein conjugates, are present in nearly every cellular compartment, with overlapping protein domain structure, localization, and functions. We discovered a cohort of DUBs that are involved in membrane trafficking (ubp4, ubp5, ubp9, ubp15, and sst2) and found that loss of all five of these DUBs but not loss of any combination of four, significantly impacted cell viability in the fission yeast Schizosaccharomyces pombe (1). Here, we delineate the collective and individual functions and activities of these five conserved DUBs using comparative proteomics, biochemistry, and microscopy. We find these five DUBs are degenerate rather than redundant at the levels of cell morphology, substrate selectivity, ubiquitin chain specificity, and cell viability under stress. These studies reveal the complexity of interplay among these enzymes, providing a foundation for understanding DUB biology and providing another example of how cells utilize degeneracy to improve survival. PMID:26412298

  11. Alternative paradigms for ion channelopathies: disorders of ion channel membrane trafficking and posttranslational modification.

    PubMed

    Curran, Jerry; Mohler, Peter J

    2015-01-01

    Channelopathies are a diverse set of disorders associated with defects in ion channel (and transporter) function. Although the vast majority of channelopathies are linked with inherited mutations that alter ion channel biophysical properties, another group of similar disorders has emerged that alter ion channel synthesis, membrane trafficking, and/or posttranslational modifications. In fact, some electrical and episodic disorders have now been identified that are not defects in the ion channel but instead reflect dysfunction in an ion channel (or transporter) regulatory protein. This review focuses on alternative paradigms for physiological disorders associated with protein biosynthesis, folding, trafficking, and membrane retention. Furthermore, the review highlights the role of aberrant posttranslational modifications in acquired channelopathies.

  12. Glut4 palmitoylation at Cys223 plays a critical role in Glut4 membrane trafficking.

    PubMed

    Ren, Wenying; Sun, Yingmin; Du, Keyong

    2015-05-08

    Recently, we identified Glut4 as a palmitoylated protein in adipocytes. To understand the role of Glut4 palmitoylation in Glut4 membrane trafficking, a process that is essential for maintenance of whole body glucose homeostasis, we have characterized Glut4 palmitoylation. We found that Glut4 is palmitoylated at Cys223 and Glut4 palmitoylation at Cys223 is essential for insulin dependent Glut4 membrane translocation as substitution of Cys223 with a serine residue in Glut4 (C223S Glut4) diminished Glut4 responsiveness to insulin in membrane translocation in both adipocytes and CHO-IR cells. We have examined C223S Glut4 subcellular localization and observed that it was absence from tubular-vesicle structure, where insulin responsive Glut4 vesicles were presented. Together, our studies uncover a novel mechanism under which Glut4 palmitoylation regulates Glut4 sorting to insulin responsive vesicles, thereby insulin-dependent Glut4 membrane translocation.

  13. Tracing the Archaeal Origins of Eukaryotic Membrane-Trafficking System Building Blocks.

    PubMed

    Klinger, Christen M; Spang, Anja; Dacks, Joel B; Ettema, Thijs J G

    2016-06-01

    In contrast to prokaryotes, eukaryotic cells are characterized by a complex set of internal membrane-bound compartments. A subset of these, and the protein machineries that move material between them, define the membrane-trafficking system (MTS), the emergence of which represents a landmark in eukaryotic evolution. Unlike mitochondria and plastids, MTS organelles have autogenous origins. Much of the MTS machinery is composed of building blocks, including small GTPase, coiled-coil, beta-propeller + alpha-solenoid, and longin domains. Despite the identification of prokaryotic proteins containing these domains, only few represent direct orthologues, leaving the origins and early evolution of the MTS poorly understood. Here, we present an in-depth analysis of MTS building block homologues in the composite genome of Lokiarchaeum, the recently discovered archaeal sister clade of eukaryotes, yielding several key insights. We identify two previously unreported Eukaryotic Signature Proteins; orthologues of the Gtr/Rag family GTPases, involved in target of rapamycin complex signaling, and of the RLC7 dynein component. We could not identify golgin or SNARE (coiled-coil) or beta-propeller + alpha-solenoid orthologues, nor typical MTS domain fusions, suggesting that these either were lost from Lokiarchaeum or emerged later in eukaryotic evolution. Furthermore, our phylogenetic analyses of lokiarchaeal GTPases support a split into Ras-like and Arf-like superfamilies, with different prokaryotic antecedents, before the advent of eukaryotes. While no GTPase activating proteins or exchange factors were identified, we show that Lokiarchaeum encodes numerous roadblock domain proteins and putative longin domain proteins, confirming the latter's origin from Archaea. Altogether, our study provides new insights into the emergence and early evolution of the eukaryotic membrane-trafficking system. © The Author 2016. Published by Oxford University Press on behalf of the Society for

  14. On the use of discrete steps in robot-aided flexible needle insertion.

    PubMed

    Geraldes, André A; Marinho, Murilo M; Bernardes, Mariana C; Bó, Antonio P L; Borges, Geovany A

    2013-01-01

    Needle steering devices present great potential for improving the safety and accuracy of medical interventions with percutaneous access. Despite significant advances in the field, needle steerability remains an issue to be solved by the scientific community. In this paper, we propose the use of discrete steps in flexible needle insertion, inspired by the manual procedure performed by physicians. Conceptually, the method relies in alternating between two motions: grasp-push and release-retreat. For experimental evaluation, a modified gripper is used along with a 6DOF robotic manipulator to control needle insertion velocity, rotation and grasping. Preliminary results indicate that the use of discrete steps minimizes some negative effects, such as slippage and needle buckling, observed on alternative methods, while preserving their functional advantages.

  15. EMBO workshop al fin del mundo: a meeting on membrane trafficking and its implication for polarity and diseases.

    PubMed

    Marzolo, María-Paz; Faundez, Victor; Galli, Thierry

    2015-07-01

    The EMBO worskhop at the "end of the world'" (al fin del mundo), a meeting on membrane trafficking and its implication for polarity and diseases, took place in the Chilean Patagonia surrounded by the landscapes once witnessed by Charles Darwin. The meeting showcased some of the best membrane trafficking science with an emphasis in neuroscience and disease models. Speakers from Europe, USA, South America and the graduate students behind it; embarked on an enthusiastic and eclectic dialog where a wide range of cell types, model genetic systems, and diseases where discussed. This meeting demonstrated the power of trafficking concepts to integrate diverse biology and to formulate mechanisms of normal and disease cells. © 2015 Société Française des Microscopies and Société de Biologie Cellulaire de France. Published by John Wiley & Sons Ltd.

  16. Common α2A and α2C adrenergic receptor polymorphisms do not affect plasma membrane trafficking.

    PubMed

    Hurt, Carl M; Sorensen, Matt W; Angelotti, Timothy

    2014-06-01

    Various naturally occurring polymorphic forms of human G protein-coupled receptors (GPCRs) have been identified and linked to diverse pathological diseases, including receptors for vasopressin type 2 (nephrogenic diabetes insipidus) and gonadotropin releasing hormone (hypogonadotropic hypogonadism). In most cases, polymorphic amino acid mutations disrupt protein folding, altering receptor function as well as plasma membrane expression. Other pathological GPCR variants have been found that do not alter receptor function, but instead affect only plasma membrane trafficking (e.g., delta opiate and histamine type 1 receptors). Thus, altered membrane trafficking with retained receptor function may be another mechanism causing polymorphic GPCR dysfunction. Two common human α2A and α2C adrenergic receptor (AR) variants have been identified (α2A N251K and α2C Δ322-325 ARs), but pharmacological analysis of ligand binding and second messenger signaling has not consistently demonstrated altered receptor function. However, possible alterations in plasma membrane trafficking have not been investigated. We utilized a systematic approach previously developed for the study of GPCR trafficking motifs and accessory proteins to assess whether these α2 AR variants affected intracellular trafficking or plasma membrane expression. By combining immunofluorescent microscopy, glycosidic processing analysis, and quantitative fluorescent-activated cell sorting (FACS), we demonstrate that neither variant receptor had altered intracellular localization, glycosylation, nor plasma membrane expression compared to wild-type α2 ARs. Therefore, pathopharmacological properties of α2A N251K and α2C Δ322-325 ARs do not appear to be due to altered receptor pharmacology or plasma membrane trafficking, but may involve interactions with other intracellular signaling cascades or proteins.

  17. Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor–mediated inflammatory responses

    PubMed Central

    Krautkrämer, Martina

    2017-01-01

    Subcellular compartmentalization of receptor signaling is an emerging principle in innate immunity. However, the functional integration of receptor signaling pathways into membrane trafficking routes and its physiological relevance for immune responses is still largely unclear. In this study, using Lyst-mutant beige mice, we show that lysosomal trafficking regulator Lyst links endolysosomal organization to the selective control of toll-like receptor 3 (TLR3)– and TLR4-mediated proinflammatory responses. Consequently, Lyst-mutant mice showed increased susceptibility to bacterial infection and were largely resistant to endotoxin-induced septic shock. Mechanistic analysis revealed that Lyst specifically controls TLR3- and TLR4-induced endosomal TRIF (TIR domain–containing adapter-inducing interferon β) signaling pathways. Loss of functional Lyst leads to dysregulated phagosomal maturation, resulting in a failure to form an activation-induced Rab7+ endosomal/phagosomal compartment. This specific Rab7+ compartment was further demonstrated to serve as a major site for active TRIF signaling events, thus linking phagosomal maturation to specific TLR signaling pathways. The immunoregulatory role of Lyst on TLR signaling pathways was confirmed in human cells by CRISPR/Cas9-mediated gene inactivation. As mutations in LYST cause human Chédiak-Higashi syndrome, a severe immunodeficiency, our findings also contribute to a better understanding of human disease mechanisms. PMID:27881733

  18. Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor-mediated inflammatory responses.

    PubMed

    Westphal, Andreas; Cheng, Weijia; Yu, Jinbo; Grassl, Guntram; Krautkrämer, Martina; Holst, Otto; Föger, Niko; Lee, Kyeong-Hee

    2017-01-01

    Subcellular compartmentalization of receptor signaling is an emerging principle in innate immunity. However, the functional integration of receptor signaling pathways into membrane trafficking routes and its physiological relevance for immune responses is still largely unclear. In this study, using Lyst-mutant beige mice, we show that lysosomal trafficking regulator Lyst links endolysosomal organization to the selective control of toll-like receptor 3 (TLR3)- and TLR4-mediated proinflammatory responses. Consequently, Lyst-mutant mice showed increased susceptibility to bacterial infection and were largely resistant to endotoxin-induced septic shock. Mechanistic analysis revealed that Lyst specifically controls TLR3- and TLR4-induced endosomal TRIF (TIR domain-containing adapter-inducing interferon β) signaling pathways. Loss of functional Lyst leads to dysregulated phagosomal maturation, resulting in a failure to form an activation-induced Rab7(+) endosomal/phagosomal compartment. This specific Rab7(+) compartment was further demonstrated to serve as a major site for active TRIF signaling events, thus linking phagosomal maturation to specific TLR signaling pathways. The immunoregulatory role of Lyst on TLR signaling pathways was confirmed in human cells by CRISPR/Cas9-mediated gene inactivation. As mutations in LYST cause human Chédiak-Higashi syndrome, a severe immunodeficiency, our findings also contribute to a better understanding of human disease mechanisms. © 2016 Westphal et al.

  19. KIF13B regulates angiogenesis through Golgi to plasma membrane trafficking of VEGFR2.

    PubMed

    Yamada, Kaori H; Nakajima, Yuki; Geyer, Melissa; Wary, Kishore K; Ushio-Fukai, Masuko; Komarova, Yulia; Malik, Asrar B

    2014-10-15

    Although the trafficking of newly synthesized VEGFR2 to the plasma membrane is a key determinant of angiogenesis, the molecular mechanisms of Golgi to plasma membrane trafficking are unknown. Here, we have identified a key role of the kinesin family plus-end molecular motor KIF13B in delivering VEGFR2 cargo from the Golgi to the endothelial cell surface. KIF13B is shown to interact directly with VEGFR2 on microtubules. We also observed that overexpression of truncated versions of KIF13B containing the binding domains that interact with VEGFR2 inhibited VEGF-induced capillary tube formation. KIF13B depletion prevented VEGF-mediated endothelial migration, capillary tube formation and neo-vascularization in mice. Impairment in trafficking induced by knockdown of KIF13B shunted VEGFR2 towards the lysosomal degradation pathway. Thus, KIF13B is an essential molecular motor required for the trafficking of VEGFR2 from the Golgi, and its delivery to the endothelial cell surface mediates angiogenesis.

  20. Evolution of the eukaryotic membrane-trafficking system: origin, tempo and mode.

    PubMed

    Dacks, Joel B; Field, Mark C

    2007-09-01

    The emergence of an endomembrane system was a crucial stage in the prokaryote-to-eukaryote evolutionary transition. Recent genomic and molecular evolutionary analyses have provided insight into how this critical system arrived at its modern configuration. The apparent relative absence of prokaryotic antecedents for the endomembrane machinery contrasts with the situation for mitochondria, plastids and the nucleus. Overall, the evidence suggests an autogenous origin for the eukaryotic membrane-trafficking machinery. The emerging picture is that early eukaryotic ancestors had a complex endomembrane system, which implies that this cellular system evolved relatively rapidly after the proto-eukaryote diverged away from the other prokaryotic lines. Many of the components of the trafficking system are the result of gene duplications that have produced proteins that have similar functions but differ in their subcellular location. A proto-eukaryote possessing a very simple trafficking system could thus have evolved to near modern complexity in the last common eukaryotic ancestor (LCEA) via paralogous gene family expansion of the proteins encoding organelle identity. The descendents of this common ancestor have undergone further modification of the trafficking machinery; unicellular simplicity and multicellular complexity are the prevailing trend, but there are some remarkable counter-examples.

  1. Alterations in membrane trafficking and pathophysiological implications in lysosomal storage disorders.

    PubMed

    Kuech, Eva-Maria; Brogden, Graham; Naim, Hassan Y

    2016-11-01

    Lysosomal storage disorders are a heterogeneous group of more than 50 distinct inborn metabolic diseases affecting about 1 in 5000 to 7000 live births. The diseases often result from mutations followed by functional deficiencies of enzymes or transporters within the acidic environment of the lysosome, which mediate the degradation of a wide subset of substrates, including glycosphingolipids, glycosaminoglycans, cholesterol, glycogen, oligosaccharides, peptides and glycoproteins, or the export of the respective degradation products from the lysosomes. The progressive accumulation of uncleaved substrates occurs in multiple organs and finally causes a broad spectrum of different pathologies including visceral, neurological, skeletal and hematologic manifestations. Besides deficient lysosomal enzymes and transporters other defects may lead to lysosomal storage disorders, including activator defects, membrane defects or defects in modifier proteins. In this review we concentrate on four different lysosomal storage disorders: Niemann-Pick type C, Fabry disease, Gaucher disease and Pompe disease. While the last three are caused by defective lysosomal hydrolases, Niemann-Pick type C is caused by the inability to export LDL-derived cholesterol out of the lysosome. We want to emphasise potential implications of membrane trafficking defects on the pathology of these diseases, as many mutations interfere with correct lysosomal protein trafficking and alter cellular lipid homeostasis. Current therapeutic strategies are summarised, including substrate reduction therapy as well as pharmacological chaperone therapy which directly aim to improve folding and lysosomal transport of misfolded mutant proteins.

  2. Alternative routes to the cell surface underpin insulin-regulated membrane trafficking of GLUT4.

    PubMed

    Kioumourtzoglou, Dimitrios; Pryor, Paul R; Gould, Gwyn W; Bryant, Nia J

    2015-07-15

    Insulin-stimulated delivery of glucose transporters (GLUT4, also known as SLC2A4) from specialized intracellular GLUT4 storage vesicles (GSVs) to the surface of fat and muscle cells is central to whole-body glucose regulation. This translocation and subsequent internalization of GLUT4 back into intracellular stores transits through numerous small membrane-bound compartments (internal GLUT4-containing vesicles; IGVs) including GSVs, but the function of these different compartments is not clear. Cellugyrin (also known as synaptogyrin-2) and sortilin define distinct populations of IGV; sortilin-positive IGVs represent GSVs, but the function of cellugyrin-containing IGVs is unknown. Here, we demonstrate a role for cellugyrin in intracellular sequestration of GLUT4 in HeLa cells and have used a proximity ligation assay to follow changes in pairwise associations between cellugyrin, sortilin, GLUT4 and membrane trafficking machinery following insulin-stimulation of 3T3-L1 adipoctyes. Our data suggest that insulin stimulates traffic from cellugyrin-containing to sortilin-containing membranes, and that cellugyrin-containing IGVs provide an insulin-sensitive reservoir to replenish GSVs following insulin-stimulated exocytosis of GLUT4. Furthermore, our data support the existence of a pathway from cellugyrin-containing membranes to the surface of 3T3-L1 adipocytes that bypasses GSVs under basal conditions, and that insulin diverts traffic away from this into GSVs.

  3. Alternative routes to the cell surface underpin insulin-regulated membrane trafficking of GLUT4

    PubMed Central

    Kioumourtzoglou, Dimitrios; Pryor, Paul R.; Gould, Gwyn W.; Bryant, Nia J.

    2015-01-01

    ABSTRACT Insulin-stimulated delivery of glucose transporters (GLUT4, also known as SLC2A4) from specialized intracellular GLUT4 storage vesicles (GSVs) to the surface of fat and muscle cells is central to whole-body glucose regulation. This translocation and subsequent internalization of GLUT4 back into intracellular stores transits through numerous small membrane-bound compartments (internal GLUT4-containing vesicles; IGVs) including GSVs, but the function of these different compartments is not clear. Cellugyrin (also known as synaptogyrin-2) and sortilin define distinct populations of IGV; sortilin-positive IGVs represent GSVs, but the function of cellugyrin-containing IGVs is unknown. Here, we demonstrate a role for cellugyrin in intracellular sequestration of GLUT4 in HeLa cells and have used a proximity ligation assay to follow changes in pairwise associations between cellugyrin, sortilin, GLUT4 and membrane trafficking machinery following insulin-stimulation of 3T3-L1 adipoctyes. Our data suggest that insulin stimulates traffic from cellugyrin-containing to sortilin-containing membranes, and that cellugyrin-containing IGVs provide an insulin-sensitive reservoir to replenish GSVs following insulin-stimulated exocytosis of GLUT4. Furthermore, our data support the existence of a pathway from cellugyrin-containing membranes to the surface of 3T3-L1 adipocytes that bypasses GSVs under basal conditions, and that insulin diverts traffic away from this into GSVs. PMID:26071524

  4. Calculation of Fresnel diffraction from 1D phase step by discrete Fourier transform

    NASA Astrophysics Data System (ADS)

    Aalipour, Rasoul

    2017-01-01

    When a part of an optical wave-front experiences a sharp change in its phase, Fresnel diffraction becomes appreciable. Sharp change in phase occurs as a wave-front strikes with a phase step. The intensity distributions of diffraction patterns of the phase step is formulated by applying Fresnel-Kirchhoff integral. For while the incident light on the step is coherent, the Fresnel-Kirchhoff integral can be solved by using familiar Fresnel integrals. But, when the incident light is partially coherent, one can not express the diffraction integral as the Fresnel integrals and the problem is summarized in solving some unusual integrals. In this report, we propose Fourier transform method for solving the Fresnel-Kirchhoff integral. In this regard we use discrete Fourier transform method and calculate Fresnel diffraction from the 1D phase step by FFT-based algorithms. This method does not have any restriction on the coherence and profile shape of the incident light. We show that the method have appropriate solutions for coherent and partially coherent lights. For the case of the coherent light illumination of the step, the obtained results are in good agreement with the calculated results by using the Fresnel integrals in reported literatures.

  5. Single-trial Spike Trains in Parietal Cortex Reveal Discrete Steps During Decision-making

    PubMed Central

    Latimer, Kenneth W.; Yates, Jacob L.; Meister, Miriam L. R.; Huk, Alexander C.; Pillow, Jonathan W.

    2016-01-01

    Neurons in the macaque lateral intraparietal (LIP) area exhibit firing rates that appear to ramp upwards or downwards during decision-making. These ramps are commonly assumed to reflect the gradual accumulation of evidence towards a decision threshold. However, the ramping in trial-averaged responses could instead arise from instantaneous jumps at different times on different trials. We examined single-trial responses in LIP using statistical methods for fitting and comparing latent dynamical spike train models. We compared models with latent spike rates governed by either continuous diffusion-to-bound dynamics or discrete “stepping” dynamics. Roughly three-quarters of the choice-selective neurons we recorded were better described by the stepping model. Moreover, the inferred steps carried more information about the animal’s choice than spike counts. PMID:26160947

  6. Continuous sweep versus discrete step protocols for studying effects of wearable robot assistance magnitude.

    PubMed

    Malcolm, Philippe; Rossi, Denise Martineli; Siviy, Christopher; Lee, Sangjun; Quinlivan, Brendan Thomas; Grimmer, Martin; Walsh, Conor J

    2017-07-12

    Different groups developed wearable robots for walking assistance, but there is still a need for methods to quickly tune actuation parameters for each robot and population or sometimes even for individual users. Protocols where parameters are held constant for multiple minutes have traditionally been used for evaluating responses to parameter changes such as metabolic rate or walking symmetry. However, these discrete protocols are time-consuming. Recently, protocols have been proposed where a parameter is changed in a continuous way. The aim of the present study was to compare effects of continuously varying assistance magnitude with a soft exosuit against discrete step conditions. Seven participants walked on a treadmill wearing a soft exosuit that assists plantarflexion and hip flexion. In Continuous-up, peak exosuit ankle moment linearly increased from approximately 0 to 38% of biological moment over 10 min. Continuous-down was the opposite. In Discrete, participants underwent five periods of 5 min with steady peak moment levels distributed over the same range as Continuous-up and Continuous-down. We calculated metabolic rate for the entire Continuous-up and Continuous-down conditions and the last 2 min of each Discrete force level. We compared kinematics, kinetics and metabolic rate between conditions by curve fitting versus peak moment. Reduction in metabolic rate compared to Powered-off was smaller in Continuous-up than in Continuous-down at most peak moment levels, due to physiological dynamics causing metabolic measurements in Continuous-up and Continuous-down to lag behind the values expected during steady-state testing. When evaluating the average slope of metabolic reduction over the entire peak moment range there was no significant difference between Continuous-down and Discrete. Attempting to correct the lag in metabolics by taking the average of Continuous-up and Continuous-down removed all significant differences versus Discrete. For kinematic and

  7. Discrete Step Sizes of Molecular Motors Lead to Bimodal Non-Gaussian Velocity Distributions under Force.

    PubMed

    Vu, Huong T; Chakrabarti, Shaon; Hinczewski, Michael; Thirumalai, D

    2016-08-12

    Fluctuations in the physical properties of biological machines are inextricably linked to their functions. Distributions of run lengths and velocities of processive molecular motors, like kinesin-1, are accessible through single-molecule techniques, but rigorous theoretical models for these probabilities are lacking. Here, we derive exact analytic results for a kinetic model to predict the resistive force (F)-dependent velocity [P(v)] and run length [P(n)] distribution functions of generic finitely processive molecular motors. Our theory quantitatively explains the zero force kinesin-1 data for both P(n) and P(v) using the detachment rate as the only parameter. In addition, we predict the F dependence of these quantities. At nonzero F, P(v) is non-Gaussian and is bimodal with peaks at positive and negative values of v, which is due to the discrete step size of kinesin-1. Although the predictions are based on analyses of kinesin-1 data, our results are general and should hold for any processive motor, which walks on a track by taking discrete steps.

  8. Discrete Step Sizes of Molecular Motors Lead to Bimodal Non-Gaussian Velocity Distributions under Force

    NASA Astrophysics Data System (ADS)

    Vu, Huong T.; Chakrabarti, Shaon; Hinczewski, Michael; Thirumalai, D.

    2016-08-01

    Fluctuations in the physical properties of biological machines are inextricably linked to their functions. Distributions of run lengths and velocities of processive molecular motors, like kinesin-1, are accessible through single-molecule techniques, but rigorous theoretical models for these probabilities are lacking. Here, we derive exact analytic results for a kinetic model to predict the resistive force (F )-dependent velocity [P (v )] and run length [P (n )] distribution functions of generic finitely processive molecular motors. Our theory quantitatively explains the zero force kinesin-1 data for both P (n ) and P (v ) using the detachment rate as the only parameter. In addition, we predict the F dependence of these quantities. At nonzero F , P (v ) is non-Gaussian and is bimodal with peaks at positive and negative values of v , which is due to the discrete step size of kinesin-1. Although the predictions are based on analyses of kinesin-1 data, our results are general and should hold for any processive motor, which walks on a track by taking discrete steps.

  9. A new theory for multistep discretizations of stiff ordinary differential equations: Stability with large step sizes

    NASA Technical Reports Server (NTRS)

    Majda, G.

    1985-01-01

    A large set of variable coefficient linear systems of ordinary differential equations which possess two different time scales, a slow one and a fast one is considered. A small parameter epsilon characterizes the stiffness of these systems. A system of o.d.e.s. in this set is approximated by a general class of multistep discretizations which includes both one-leg and linear multistep methods. Sufficient conditions are determined under which each solution of a multistep method is uniformly bounded, with a bound which is independent of the stiffness of the system of o.d.e.s., when the step size resolves the slow time scale, but not the fast one. This property is called stability with large step sizes. The theory presented lets one compare properties of one-leg methods and linear multistep methods when they approximate variable coefficient systems of stiff o.d.e.s. In particular, it is shown that one-leg methods have better stability properties with large step sizes than their linear multistep counter parts. The theory also allows one to relate the concept of D-stability to the usual notions of stability and stability domains and to the propagation of errors for multistep methods which use large step sizes.

  10. COPI-mediated membrane trafficking is required for cytokinesis in Drosophila male meiotic divisions.

    PubMed

    Kitazawa, Daishi; Yamaguchi, Masamitsu; Mori, Hajime; Inoue, Yoshihiro H

    2012-08-01

    The coatomer protein complex, COPI, mediates retrograde vesicle transport from the Golgi apparatus to the ER. Here, we investigated the meiotic phenotype of Drosophila melanogaster spermatocytes expressing dsRNA of 52 genes encoding membrane-trafficking-related factors. We identified COPI as an essential factor for male meiosis. In Drosophila male meiotic divisions, COPI is localized in the ER-Golgi intermediate compartment of tER-Golgi units scattered throughout the spermatocyte cytoplasm. Prior to chromosome segregation, the vesicles assemble at the spindle pole periphery through a poleward movement, mediated by minus-end motor dynein along astral microtubules. At the end of each meiotic division, COPI-containing vesicles are equally partitioned between two daughter cells. Our present data strongly suggest that spermatocytes possess a regulatory mechanism for equal inheritance of several types of membrane vesicles. Using testis-specific knockdown of COPI subunits or the small GTPase Arf or mutations of the γCOP gene, we examined the role of COPI in male meiosis. COPI depletion resulted in the failure of cytokinesis, through disrupted accumulation of essential proteins and lipid components at the cleavage furrow region. Furthermore, it caused a reduction in the number of overlapping central spindle microtubules, which are essential for cytokinesis. Drosophila spermatocytes construct ER-based intracellular structures associated with astral and spindle microtubules. COPI depletion resulted in severe disruption of these ER-based structures. Thus, we propose that COPI plays an important role in Drosophila male meiosis, not only through vesicle transport to the cleavage furrow region, but also through the formation of ER-based structures.

  11. Estradiol regulates human QT-interval: acceleration of cardiac repolarization by enhanced KCNH2 membrane trafficking.

    PubMed

    Anneken, Lars; Baumann, Stefan; Vigneault, Patrick; Biliczki, Peter; Friedrich, Corinna; Xiao, Ling; Girmatsion, Zenawit; Takac, Ina; Brandes, Ralf P; Kissler, Stefan; Wiegratz, Inka; Zumhagen, Sven; Stallmeyer, Birgit; Hohnloser, Stefan H; Klingenheben, Thomas; Schulze-Bahr, Eric; Nattel, Stanley; Ehrlich, Joachim R

    2016-02-14

    Modulation of cardiac repolarization by sexual hormones is controversial and hormonal effects on ion channels remain largely unknown. In the present translational study, we therefore assessed the relationship between QTc duration and gonadal hormones and studied underlying mechanisms. We measured hormone levels and QTc intervals in women during clomiphene stimulation for infertility and women before, during, and after pregnancy. Three heterozygous LQT-2 patients (KCNH2-p.Arg752Pro missense mutation) and two unaffected family members additionally were studied during their menstrual cycles. A comprehensive cellular and molecular analysis was done to identify the mechanisms of hormonal QT-interval regulation. High estradiol levels, but neither progesterone nor estradiol/progesterone ratio, inversely correlated with QTc. Consistent with clinical data, in vitro estradiol stimulation (60 pmol/L, 48 h) enhanced IKCNH2. This increase was mediated by estradiol receptor-α-dependent promotion of KCNH2-channel trafficking to the cell membrane. To study the underlying mechanism, we focused on heat-shock proteins. The heat-shock protein-90 (Hsp90) inhibitor geldanamycin abolished estradiol-induced increase in IKCNH2. Geldanamycin had no effect on KCNH2 transcription or translation; nor did it affect expression of estradiol receptors and chaperones. Estradiol enhanced the physical interaction of KCNH2-channel subunits with heat-shock proteins and augmented ion-channel trafficking to the membrane. Elevated estradiol levels were associated with shorter QTc intervals in healthy women and female LQT-2 patients. Estradiol acts on KCNH2 channels via enhanced estradiol-receptor-α-mediated Hsp90 interaction, augments membrane trafficking and thereby increases repolarizing current. These results provide mechanistic insights into hormonal control of human ventricular repolarization and open novel therapeutic avenues. Published on behalf of the European Society of Cardiology. All rights

  12. Estradiol regulates human QT-interval: acceleration of cardiac repolarization by enhanced KCNH2 membrane trafficking

    PubMed Central

    Anneken, Lars; Baumann, Stefan; Vigneault, Patrick; Biliczki, Peter; Friedrich, Corinna; Xiao, Ling; Girmatsion, Zenawit; Takac, Ina; Brandes, Ralf P.; Kissler, Stefan; Wiegratz, Inka; Zumhagen, Sven; Stallmeyer, Birgit; Hohnloser, Stefan H.; Klingenheben, Thomas; Schulze-Bahr, Eric; Nattel, Stanley; Ehrlich, Joachim R.

    2016-01-01

    Background Modulation of cardiac repolarization by sexual hormones is controversial and hormonal effects on ion channels remain largely unknown. In the present translational study, we therefore assessed the relationship between QTc duration and gonadal hormones and studied underlying mechanisms. Methods and results We measured hormone levels and QTc intervals in women during clomiphene stimulation for infertility and women before, during, and after pregnancy. Three heterozygous LQT-2 patients (KCNH2-p.Arg752Pro missense mutation) and two unaffected family members additionally were studied during their menstrual cycles. A comprehensive cellular and molecular analysis was done to identify the mechanisms of hormonal QT-interval regulation. High estradiol levels, but neither progesterone nor estradiol/progesterone ratio, inversely correlated with QTc. Consistent with clinical data, in vitro estradiol stimulation (60 pmol/L, 48 h) enhanced IKCNH2. This increase was mediated by estradiol receptor-α-dependent promotion of KCNH2-channel trafficking to the cell membrane. To study the underlying mechanism, we focused on heat-shock proteins. The heat-shock protein-90 (Hsp90) inhibitor geldanamycin abolished estradiol-induced increase in IKCNH2. Geldanamycin had no effect on KCNH2 transcription or translation; nor did it affect expression of estradiol receptors and chaperones. Estradiol enhanced the physical interaction of KCNH2-channel subunits with heat-shock proteins and augmented ion-channel trafficking to the membrane. Conclusion Elevated estradiol levels were associated with shorter QTc intervals in healthy women and female LQT-2 patients. Estradiol acts on KCNH2 channels via enhanced estradiol-receptor-α-mediated Hsp90 interaction, augments membrane trafficking and thereby increases repolarizing current. These results provide mechanistic insights into hormonal control of human ventricular repolarization and open novel therapeutic avenues. PMID:26271031

  13. Comment on "Single-trial spike trains in parietal cortex reveal discrete steps during decision-making".

    PubMed

    Shadlen, Michael N; Kiani, Roozbeh; Newsome, William T; Gold, Joshua I; Wolpert, Daniel M; Zylberberg, Ariel; Ditterich, Jochen; de Lafuente, Victor; Yang, Tianming; Roitman, Jamie

    2016-03-25

    Latimeret al (Reports, 10 July 2015, p. 184) claim that during perceptual decision formation, parietal neurons undergo one-time, discrete steps in firing rate instead of gradual changes that represent the accumulation of evidence. However, that conclusion rests on unsubstantiated assumptions about the time window of evidence accumulation, and their stepping model cannot explain existing data as effectively as evidence-accumulation models.

  14. Permeability and kinetic coefficients for mesoscale BCF surface step dynamics: Discrete two-dimensional deposition-diffusion equation analysis

    SciTech Connect

    Zhao, Renjie; Evans, James W.; Oliveira, Tiago J.

    2016-04-08

    Here, a discrete version of deposition-diffusion equations appropriate for description of step flow on a vicinal surface is analyzed for a two-dimensional grid of adsorption sites representing the stepped surface and explicitly incorporating kinks along the step edges. Model energetics and kinetics appropriately account for binding of adatoms at steps and kinks, distinct terrace and edge diffusion rates, and possible additional barriers for attachment to steps. Analysis of adatom attachment fluxes as well as limiting values of adatom densities at step edges for nonuniform deposition scenarios allows determination of both permeability and kinetic coefficients. Behavior of these quantities is assessed as a function of key system parameters including kink density, step attachment barriers, and the step edge diffusion rate.

  15. Permeability and kinetic coefficients for mesoscale BCF surface step dynamics: Discrete two-dimensional deposition-diffusion equation analysis

    DOE PAGES

    Zhao, Renjie; Evans, James W.; Oliveira, Tiago J.

    2016-04-08

    Here, a discrete version of deposition-diffusion equations appropriate for description of step flow on a vicinal surface is analyzed for a two-dimensional grid of adsorption sites representing the stepped surface and explicitly incorporating kinks along the step edges. Model energetics and kinetics appropriately account for binding of adatoms at steps and kinks, distinct terrace and edge diffusion rates, and possible additional barriers for attachment to steps. Analysis of adatom attachment fluxes as well as limiting values of adatom densities at step edges for nonuniform deposition scenarios allows determination of both permeability and kinetic coefficients. Behavior of these quantities is assessedmore » as a function of key system parameters including kink density, step attachment barriers, and the step edge diffusion rate.« less

  16. Discrete Topics in the Undergraduate Mathematics Curriculum: How Big a Step Should We Take?

    ERIC Educational Resources Information Center

    Gordon, Sheldon P.

    The question of the advisability of incorporating discrete mathematics into the mathematics curriculum is addressed by examining the different types of courses typically offered in the first two years of college and the appropriateness of including discrete mathematics topics in these courses. The introductory section explains how the advent of…

  17. Discrete Topics in the Undergraduate Mathematics Curriculum: How Big a Step Should We Take?

    ERIC Educational Resources Information Center

    Gordon, Sheldon P.

    The question of the advisability of incorporating discrete mathematics into the mathematics curriculum is addressed by examining the different types of courses typically offered in the first two years of college and the appropriateness of including discrete mathematics topics in these courses. The introductory section explains how the advent of…

  18. Seeing the endomembrane system for the trees: Evolutionary analysis highlights the importance of plants as models for eukaryotic membrane-trafficking.

    PubMed

    Barlow, L D; Dacks, J B

    2017-09-19

    Plant cells show many signs of a unique evolutionary history. This is seen in the system of intracellular organelles and vesicle transport pathways plants use to traffic molecular cargo. Bioinformatic and cell biological work in this area is beginning to tackle the question of how plant cells have evolved, and what this tells us about the evolution of other eukaryotes. Key protein families with membrane trafficking function, including Rabs, SNAREs, vesicle coat proteins, and ArfGAPs, show patterns of evolution that indicate both specialization and conservation in plants. These changes are accompanied by changes at the level of organelles and trafficking pathways between them. Major specializations include losses of several ancient Rabs, novel functions of many proteins, and apparent modification of trafficking in endocytosis and cytokinesis. Nevertheless, plants show extensive conservation of ancestral membrane trafficking genes, and conservation of their ancestral function in most duplicates. Moreover, plants have retained several ancient membrane trafficking genes lost in the evolution of animals and fungi. Considering this, plants such as Arabidopsis are highly valuable for investigating not only plant-specific aspects of membrane trafficking, but also general eukaryotic mechanisms. Copyright © 2017. Published by Elsevier Ltd.

  19. Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells.

    PubMed

    Sato, Sei-ichi; Inoue, Hiroki; Kogure, Takeshi; Tagaya, Mitsuo; Tani, Katsuko

    2010-11-05

    Mammals have three members of the intracellular phospholipase A(1) protein family (phosphatidic acid preferring-phospholipase A(1), p125, and KIAA0725p). In this study, we showed that KIAA0725p is localized in the Golgi, and is rapidly cycled between the Golgi and cytosol. Catalytic activity is important for targeting of KIAA0725p to Golgi membranes. RNA interference experiments suggested that KIAA0725p contributes to efficient membrane trafficking from the Golgi apparatus to the plasma membrane, but is not involved in brefeldin A-induced Golgi-to-endoplasmic reticulum retrograde transport. Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  20. Drosophila Cip4/Toca-1 integrates membrane trafficking and actin dynamics through WASP and SCAR/WAVE.

    PubMed

    Fricke, Robert; Gohl, Christina; Dharmalingam, Elavarasi; Grevelhörster, Astrid; Zahedi, Baharak; Harden, Nicholas; Kessels, Michael; Qualmann, Britta; Bogdan, Sven

    2009-09-15

    Developmental processes are intimately tied to signaling events that integrate the dynamic reorganization of the actin cytoskeleton and membrane dynamics. The F-BAR-domain-containing proteins are prime candidates to couple actin dynamics and membrane trafficking in different morphogenetic processes. Here, we present the functional analysis of the Drosophila F-BAR protein Cip4/Toca1 (Cdc42-interacting protein 4/transducer of Cdc42-dependent actin assembly 1). Cip4 is able to form a complex with WASP and SCAR/WAVE and recruits both actin-nucleation-promoting factors to invaginating membranes and endocytic vesicles. Actin-comet-tail-based movement of these vesicles depends not only on WASP but largely on WAVE function. In vivo, loss of cip4 function causes multiple wing hairs. A similar phenotype is observed when vesicle scission is affected after Dynamin suppression. Gene dosage experiments show that Cip4 and WAVE functionally interact to restrict wing hair formation. Further rescue experiments confirm that Cip4 is able to act through WAVE and WASP in vivo. Biochemical and functional data support a model in which Cdc42 acts upstream of Cip4 and recruits not only WASP but also SCAR/WAVE via Abi to control Dynamin-dependent cell polarization in the wing. Cip4 integrates membrane trafficking and actin dynamics through WASP and WAVE. First, Cip4 promotes membrane invaginations and triggers the vesicle scission by recruiting Dynamin to the neck of nascent vesicles. Second, Cip4 recruits WASP and WAVE proteins to induce actin polymerization, supporting vesicle scission and providing the force for vesicle movement.

  1. Imp2, the PSTPIP homolog in fission yeast, affects sensitivity to the immunosuppressant FK506 and membrane trafficking in fission yeast

    SciTech Connect

    Kita, Ayako; Higa, Mari; Doi, Akira; Satoh, Ryosuke; Sugiura, Reiko

    2015-02-13

    Cytokinesis is a highly ordered process that divides one cell into two cells, which is functionally linked to the dynamic remodeling of the plasma membrane coordinately with various events such as membrane trafficking. Calcineurin is a highly conserved serine/threonine protein phosphatase, which regulates multiple biological functions, such as membrane trafficking and cytokinesis. Here, we isolated imp2-c3, a mutant allele of the imp2{sup +} gene, encoding a homolog of the mouse PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1), using a genetic screen for mutations that are synthetically lethal with calcineurin deletion in fission yeast. The imp2-c3 mutants showed a defect in cytokinesis with multi-septated phenotypes, which was further enhanced upon treatment with the calcineurin inhibitor FK506. Notably, electron micrographs revealed that the imp2-c3 mutant cells accumulated aberrant multi-lamella Golgi structures and putative post-Golgi secretory vesicles, and exhibited fragmented vacuoles in addition to thickened septa. Consistently, imp2-c3 mutants showed a reduced secretion of acid phosphatase and defects in vacuole fusion. The imp2-c3 mutant cells exhibited a weakened cell wall, similar to the membrane trafficking mutants identified in the same genetic screen such as ypt3-i5. These findings implicate the PSTPIP1 homolog Imp2 in Golgi/vacuole function, thereby affecting various cellular processes, including cytokinesis and cell integrity. - Highlights: • We isolated imp2-c3, in a synthetic lethal screen with calcineurin in fission yeast. • The imp2{sup +} gene encodes a component of the actin contractile ring similar to Cdc15. • The imp2-c3 mutants showed defects in cytokinesis, which were exacerbated by FK506. • The imp2-c3 mutants were defective in membrane trafficking and cell wall integrity. • Our study revealed a novel role for Imp2 in the Golgi/vacuolar membrane trafficking.

  2. Matrix approach to discrete fractional calculus III: non-equidistant grids, variable step length and distributed orders.

    PubMed

    Podlubny, Igor; Skovranek, Tomas; Vinagre Jara, Blas M; Petras, Ivo; Verbitsky, Viktor; Chen, YangQuan

    2013-05-13

    In this paper, we further develop Podlubny's matrix approach to discretization of integrals and derivatives of non-integer order. Numerical integration and differentiation on non-equidistant grids is introduced and illustrated by several examples of numerical solution of differential equations with fractional derivatives of constant orders and with distributed-order derivatives. In this paper, for the first time, we present a variable-step-length approach that we call 'the method of large steps', because it is applied in combination with the matrix approach for each 'large step'. This new method is also illustrated by an easy-to-follow example. The presented approach allows fractional-order and distributed-order differentiation and integration of non-uniformly sampled signals, and opens the way to development of variable- and adaptive-step-length techniques for fractional- and distributed-order differential equations.

  3. Parallel machine scheduling with step-deteriorating jobs and setup times by a hybrid discrete cuckoo search algorithm

    NASA Astrophysics Data System (ADS)

    Guo, Peng; Cheng, Wenming; Wang, Yi

    2015-11-01

    This article considers the parallel machine scheduling problem with step-deteriorating jobs and sequence-dependent setup times. The objective is to minimize the total tardiness by determining the allocation and sequence of jobs on identical parallel machines. In this problem, the processing time of each job is a step function dependent upon its starting time. An individual extended time is penalized when the starting time of a job is later than a specific deterioration date. The possibility of deterioration of a job makes the parallel machine scheduling problem more challenging than ordinary ones. A mixed integer programming model for the optimal solution is derived. Due to its NP-hard nature, a hybrid discrete cuckoo search algorithm is proposed to solve this problem. In order to generate a good initial swarm, a modified Biskup-Hermann-Gupta (BHG) heuristic called MBHG is incorporated into the population initialization. Several discrete operators are proposed in the random walk of Lévy flights and the crossover search. Moreover, a local search procedure based on variable neighbourhood descent is integrated into the algorithm as a hybrid strategy in order to improve the quality of elite solutions. Computational experiments are executed on two sets of randomly generated test instances. The results show that the proposed hybrid algorithm can yield better solutions in comparison with the commercial solver CPLEX® with a one hour time limit, the discrete cuckoo search algorithm and the existing variable neighbourhood search algorithm.

  4. The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways

    PubMed Central

    Alto, Neal M.; Weflen, Andrew W.; Rardin, Matthew J.; Yarar, Defne; Lazar, Cheri S.; Tonikian, Raffi; Koller, Antonius; Taylor, Susan S.; Boone, Charles; Sidhu, Sachdev S.; Schmid, Sandra L.; Hecht, Gail A.; Dixon, Jack E.

    2007-01-01

    Bacterial toxins and effector proteins hijack eukaryotic enzymes that are spatially localized and display rapid signaling kinetics. However, the molecular mechanisms by which virulence factors engage highly dynamic substrates in the host cell environment are poorly understood. Here, we demonstrate that the enteropathogenic Escherichia coli (EPEC) type III effector protein EspF nucleates a multiprotein signaling complex composed of eukaryotic sorting nexin 9 (SNX9) and neuronal Wiskott-Aldrich syndrome protein (N-WASP). We demonstrate that a specific and high affinity association between EspF and SNX9 induces membrane remodeling in host cells. These membrane-remodeling events are directly coupled to N-WASP/Arp2/3–mediated actin nucleation. In addition to providing a biochemical mechanism of EspF function, we find that EspF dynamically localizes to membrane-trafficking organelles in a spatiotemporal pattern that correlates with SNX9 and N-WASP activity in living cells. Thus, our findings suggest that the EspF-dependent assembly of SNX9 and N-WASP represents a novel form of signaling mimicry used to promote EPEC pathogenesis and gastrointestinal disease. PMID:17893247

  5. Membrane trafficking and mitochondrial abnormalities precede subunit c deposition in a cerebellar cell model of juvenile neuronal ceroid lipofuscinosis

    PubMed Central

    Fossale, Elisa; Wolf, Pavlina; Espinola, Janice A; Lubicz-Nawrocka, Tanya; Teed, Allison M; Gao, Hanlin; Rigamonti, Dorotea; Cattaneo, Elena; MacDonald, Marcy E; Cotman, Susan L

    2004-01-01

    Background JNCL is a recessively inherited, childhood-onset neurodegenerative disease most-commonly caused by a ~1 kb CLN3 mutation. The resulting loss of battenin activity leads to deposition of mitochondrial ATP synthase, subunit c and a specific loss of CNS neurons. We previously generated Cln3Δex7/8 knock-in mice, which replicate the common JNCL mutation, express mutant battenin and display JNCL-like pathology. Results To elucidate the consequences of the common JNCL mutation in neuronal cells, we used P4 knock-in mouse cerebella to establish conditionally immortalized CbCln3 wild-type, heterozygous, and homozygous neuronal precursor cell lines, which can be differentiated into MAP-2 and NeuN-positive, neuron-like cells. Homozygous CbCln3Δex7/8 precursor cells express low levels of mutant battenin and, when aged at confluency, accumulate ATPase subunit c. Recessive phenotypes are also observed at sub-confluent growth; cathepsin D transport and processing are altered, although enzyme activity is not significantly affected, lysosomal size and distribution are altered, and endocytosis is reduced. In addition, mitochondria are abnormally elongated, cellular ATP levels are decreased, and survival following oxidative stress is reduced. Conclusions These findings reveal that battenin is required for intracellular membrane trafficking and mitochondrial function. Moreover, these deficiencies are likely to be early events in the JNCL disease process and may particularly impact neuronal survival. PMID:15588329

  6. A complex network of interactions between mitotic kinases, phosphatases and ESCRT proteins regulates septation and membrane trafficking in S. pombe.

    PubMed

    Bhutta, Musab S; Roy, Brinta; Gould, Gwyn W; McInerny, Christopher J

    2014-01-01

    Cytokinesis and cell separation are critical events in the cell cycle. We show that Endosomal Sorting Complex Required for Transport (ESCRT) genes are required for cell separation in Schizosaccharomyces pombe. We identify genetic interactions between ESCRT proteins and polo and aurora kinases and Cdc14 phosphatase that manifest as impaired growth and exacerbated defects in septation, suggesting that the encoded proteins function together to control these processes. Furthermore, we observed defective endosomal sorting in mutants of plo1, ark1 and clp1, as has been reported for ESCRT mutants, consistent with a role for these kinases in the control of ESCRT function in membrane traffic. Multiple observations indicate functional interplay between polo and ESCRT components: firstly, two-hybrid in vivo interactions are reported between Plo1p and Sst4p, Vps28p, Vps25p, Vps20p and Vps32p; secondly, co-immunoprecipitation of human homologues of Vps20p, Vps32p, Vps24p and Vps2p by human Plk1; and thirdly, in vitro phosphorylation of budding yeast Vps32p and Vps20p by polo kinase. Two-hybrid analyses also identified interactions between Ark1p and Vps20p and Vps32p, and Clp1p and Vps28p. These experiments indicate a network of interactions between ESCRT proteins, plo1, ark1 and clp1 that coordinate membrane trafficking and cell separation in fission yeast.

  7. Resolving the homology-function relationship through comparative genomics of membrane-trafficking machinery and parasite cell biology.

    PubMed

    Klinger, Christen M; Ramirez-Macias, Inmaculada; Herman, Emily K; Turkewitz, Aaron P; Field, Mark C; Dacks, Joel B

    With advances in DNA sequencing technology, it is increasingly common and tractable to informatically look for genes of interest in the genomic databases of parasitic organisms and infer cellular states. Assignment of a putative gene function based on homology to functionally characterized genes in other organisms, though powerful, relies on the implicit assumption of functional homology, i.e. that orthology indicates conserved function. Eukaryotes reveal a dazzling array of cellular features and structural organization, suggesting a concomitant diversity in their underlying molecular machinery. Significantly, examples of novel functions for pre-existing or new paralogues are not uncommon. Do these examples undermine the basic assumption of functional homology, especially in parasitic protists, which are often highly derived? Here we examine the extent to which functional homology exists between organisms spanning the eukaryotic lineage. By comparing membrane trafficking proteins between parasitic protists and traditional model organisms, where direct functional evidence is available, we find that function is indeed largely conserved between orthologues, albeit with significant adaptation arising from the unique biological features within each lineage. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. OCRL1 engages with the F-BAR protein pacsin 2 to promote biogenesis of membrane-trafficking intermediates

    PubMed Central

    Billcliff, Peter G.; Noakes, Christopher J.; Mehta, Zenobia B.; Yan, Guanhua; Mak, LokHang; Woscholski, Rudiger; Lowe, Martin

    2016-01-01

    Mutation of the inositol 5-phosphatase OCRL1 causes Lowe syndrome and Dent-2 disease. Loss of OCRL1 function perturbs several cellular processes, including membrane traffic, but the underlying mechanisms remain poorly defined. Here we show that OCRL1 is part of the membrane-trafficking machinery operating at the trans-Golgi network (TGN)/endosome interface. OCRL1 interacts via IPIP27A with the F-BAR protein pacsin 2. OCRL1 and IPIP27A localize to mannose 6-phosphate receptor (MPR)–containing trafficking intermediates, and loss of either protein leads to defective MPR carrier biogenesis at the TGN and endosomes. OCRL1 5-phosphatase activity, which is membrane curvature sensitive, is stimulated by IPIP27A-mediated engagement of OCRL1 with pacsin 2 and promotes scission of MPR-containing carriers. Our data indicate a role for OCRL1, via IPIP27A, in regulating the formation of pacsin 2–dependent trafficking intermediates and reveal a mechanism for coupling PtdIns(4,5)P2 hydrolysis with carrier biogenesis on endomembranes. PMID:26510499

  9. Resolving the homology—function relationship through comparative genomics of membrane-trafficking machinery and parasite cell biology

    PubMed Central

    Klinger, Christen M.; Ramirez-Macias, Inmaculada; Herman, Emily K.; Turkewitz, Aaron P.; Field, Mark C.; Dacks, Joel B.

    2016-01-01

    With advances in DNA sequencing technology, it is increasingly common and tractable to informatically look for genes of interest in the genomic databases of parasitic organisms and infer cellular states. Assignment of a putative gene function based on homology to functionally characterized genes in other organisms, though powerful, relies on the implicit assumption of functional homology, i.e. that orthology indicates conserved function. Eukaryotes reveal a dazzling array of cellular features and structural organization, suggesting a concomitant diversity in their underlying molecular machinery. Significantly, examples of novel functions for pre-existing or new paralogues are not uncommon. Do these examples undermine the basic assumption of functional homology, especially in parasitic protists, which are often highly derived? Here we examine the extent to which functional homology exists between organisms spanning the eukaryotic lineage. By comparing membrane trafficking proteins between parasitic protists and traditional model organisms, where direct functional evidence is available, we find that function is indeed largely conserved between orthologues, albeit with significant adaptation arising from the unique biological features within each lineage. PMID:27444378

  10. Thrifty Tbc1d1 and Tbc1d4 proteins link signalling and membrane trafficking pathways

    PubMed Central

    Koumanov, Françoise; Holman, Geoffrey D.

    2007-01-01

    Establishing a complete pathway which links occupancy of the insulin receptor to GLUT4 translocation has been particularly elusive because of the complexities involved in studying both signalling and membrane trafficking processes. However, Lienhard's group has now discovered two related molecules that could function in this linking role. These proteins, Tbc1d4 (also known as AS160) and now Tbc1d1, as reported in this issue of the Biochemical Journal, have been demonstrated to be Rab GAPs (GTPase-activating proteins) that link upstream to Akt (protein kinase B) and phosphoinositide 3-kinase and downstream to Rabs involved in trafficking of GLUT4 vesicles. The data from Leinhard and colleagues suggest that high levels of Rab GAP activity lead to suppression of GLUT4 translocation and this observation has wide significance and is likely to be relevant to the recent discovery that mutations in the Tbc1d1 gene lead to some cases of severe human obesity. PMID:17376030

  11. The membrane trafficking and functionality of the K+-Cl− co-transporter KCC2 is regulated by TGF-β2

    PubMed Central

    Speer, Jan Manuel; Chudotvorova, Ilona; Khakipoor, Shokoufeh; Rivera, Claudio; Krieglstein, Kerstin

    2016-01-01

    ABSTRACT Functional activation of the neuronal K+-Cl− co-transporter KCC2 (also known as SLC12A5) is a prerequisite for shifting GABAA responses from depolarizing to hyperpolarizing during development. Here, we introduce transforming growth factor β2 (TGF-β2) as a new regulator of KCC2 membrane trafficking and functional activation. TGF-β2 controls membrane trafficking, surface expression and activity of KCC2 in developing and mature mouse primary hippocampal neurons, as determined by immunoblotting, immunofluorescence, biotinylation of surface proteins and KCC2-mediated Cl− extrusion. We also identify the signaling pathway from TGF-β2 to cAMP-response-element-binding protein (CREB) and Ras-associated binding protein 11b (Rab11b) as the underlying mechanism for TGF-β2-mediated KCC2 trafficking and functional activation. TGF-β2 increases colocalization and interaction of KCC2 with Rab11b, as determined by 3D stimulated emission depletion (STED) microscopy and co-immunoprecipitation, respectively, induces CREB phosphorylation, and enhances Rab11b gene expression. Loss of function of either CREB1 or Rab11b suppressed TGF-β2-dependent KCC2 trafficking, surface expression and functionality. Thus, TGF-β2 is a new regulatory factor for KCC2 functional activation and membrane trafficking, and a putative indispensable molecular determinant for the developmental shift of GABAergic transmission. PMID:27505893

  12. A two-step finite volume method to discretize heterogeneous and anisotropic pressure equation on general grids

    NASA Astrophysics Data System (ADS)

    Zhang, Wenjuan; Al Kobaisi, Mohammed

    2017-10-01

    A novel Two-Step cell-centered Finite Volume Method (TSFVM) is developed in this work to discretize the heterogeneous and anisotropic pressure equation on triangular and quadrilateral grids in 2D and hexahedral and tetrahedral grids in 3D. Physical properties such as permeability and porosity are piece-wise constant on each grid cell. In the first step, the Galerkin Finite Element Method (FEM) is utilized to compute pressure solutions at all cell vertices. In the second step, pressure values at cell vertices are used to derive continuous two-point flux stencils for cell faces. Mass conservation equations are then written for each cell to obtain a system of linear equations that can be solved for pressure at cell centers. Extensive numerical experiments are carried out to test the performance of our TSFVM. In particular, we compare TSFVM with the classical Multipoint Flux Approximation (MPFA-O) method as well as a more recently developed MPFA method with full pressure support called enhanced MPFA (eMPFA). The results show that the TSFVM compares well with eMPFA for challenging test cases for which MPFA-O breaks down. Specifically, and as a significant step forward, our TSFVM is quite robust for challenging problems involving heterogeneous and highly anisotropic permeability tensors when both MPFA-O and eMPFA suffer from unphysical oscillations. Finally, the numerical convergence study demonstrates that TSFVM has comparable convergence behavior to MPFA-O method for both homogeneous and discontinuous permeability fields.

  13. Hybrid High Fidelity Modeling of Radar Scenarios Using Atemporal, Discrete Event, and Time Step Simulation

    DTIC Science & Technology

    2016-12-01

    significant predictors in themeta-model since themodel containing the new predictor statistically performs differently than themodel without the new...degree and four-degree interaction predictors fit model perform quite consistently in RMSE and prediction error rates from the confusion matrix analysis...TIME-STEP SIMULATION by Yuan-Pin Cheng December 2016 Dissertation Supervisors: Donald P. Brutzman Phillip E. Pace This dissertation was performed at the

  14. Single molecule FRET analysis of the 11 discrete steps of a DNA actuator.

    PubMed

    Hildebrandt, Lasse L; Preus, Søren; Zhang, Zhao; Voigt, Niels V; Gothelf, Kurt V; Birkedal, Victoria

    2014-06-25

    DNA hybridization allows the design and assembly of dynamic DNA-based molecular devices. Such structures usually accomplish their function by the addition of fuel strands that drive the structure from one conformation to a new one or by internal changes in DNA hybridization. We report here on the performance and robustness of one of these devices by the detailed study of a dynamic DNA actuator. The DNA actuator was chosen as a model system, as it is the device with most discrete states to date. It is able to reversibly slide between 11 different states and can in principle function both autonomously and nonautonomously. The 11 states of the actuator were investigated by single molecule Förster Resonance Energy Transfer (smFRET) microscopy to obtain information on the static and dynamic heterogeneities of the device. Our results show that the DNA actuator can be effectively locked in several conformations with the help of well-designed DNA lock strands. However, the device also shows pronounced static and dynamic heterogeneities both in the unlocked and locked modes, and we suggest possible structural models. Our study allows for the direct visualization of the conformational diversity and movement of the dynamic DNA-based device and shows that complex DNA-based devices are inherently heterogeneous. Our results also demonstrate that single molecule techniques are a powerful tool for structural dynamics studies and provide a stringent test for the performance of molecular devices made out of DNA.

  15. Filopodia act as phagocytic tentacles and pull with discrete steps and a load-dependent velocity

    PubMed Central

    Kress, Holger; Stelzer, Ernst H. K.; Holzer, Daniela; Buss, Folma; Griffiths, Gareth; Rohrbach, Alexander

    2007-01-01

    Filopodia are thin, spike-like cell surface protrusions containing bundles of parallel actin filaments. So far, filopodial dynamics has mainly been studied in the context of cell motility on coverslip-adherent filopodia by using fluorescence and differential interference contrast (DIC) microscopy. In this study, we used an optical trap and interferometric particle tracking with nanometer precision to measure the three-dimensional dynamics of macrophage filopodia, which were not attached to flat surfaces. We found that filopodia act as cellular tentacles: a few seconds after binding to a particle, filopodia retract and pull the bound particle toward the cell. We observed F-actin-dependent stepwise retraction of filopodia with a mean step size of 36 nm, suggesting molecular motor activity during filopodial pulling. Remarkably, this intracellular stepping motion, which was measured at counteracting forces of up to 19 pN, was transmitted to the extracellular tracked particle via the filopodial F-actin bundle and the cell membrane. The pulling velocity depended strongly on the counteracting force and ranged between 600 nm/s at forces <1 pN and ≈40 nm/s at forces >15 pN. This result provides an explanation of the significant differences in filopodial retraction velocities previously reported in the literature. The measured filopodial retraction force–velocity relationship is in agreement with a model for force-dependent multiple motor kinetics. PMID:17620618

  16. The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway

    PubMed Central

    Roboti, Peristera; Sato, Keisuke; Lowe, Martin

    2015-01-01

    Golgins are coiled-coil proteins that participate in membrane-tethering events at the Golgi complex. Golgin-mediated tethering is thought to be important for vesicular trafficking and Golgi organization. However, the degree to which individual golgins contribute to these processes is poorly defined, and it has been proposed that golgins act in a largely redundant manner. Previous studies on the golgin GMAP-210 (also known as TRIP11), which is mutated in the rare skeletal disorder achondrogenesis type 1A, have yielded conflicting results regarding its involvement in trafficking. Here, we re-investigated the trafficking role of GMAP-210, and found that it is indeed required for efficient trafficking in the secretory pathway. GMAP-210 acts at both the endoplasmic reticulum (ER)-to-Golgi intermediate compartment (ERGIC) and Golgi complex during anterograde trafficking, and is also required for retrograde trafficking to the ER. Using co-depletion experiments, we also found that GMAP-210 acts in a partially redundant manner with the golgin GM130 to ensure efficient anterograde cargo delivery to the cis-Golgi. In summary, our results indicate a role for GMAP-210 in several trafficking steps at the ER–Golgi interface, some of which are partially redundant with another golgin, namely GM130 (also known as GOLGA2). PMID:25717001

  17. The functions of Reelin in membrane trafficking and cytoskeletal dynamics: implications for neuronal migration, polarization and differentiation.

    PubMed

    Santana, Jessica; Marzolo, María-Paz

    2017-09-07

    Reelin is a large extracellular matrix protein with relevant roles in mammalian central nervous system including neurogenesis, neuronal polarization and migration during development; and synaptic plasticity with its implications in learning and memory, in the adult. Dysfunctions in reelin signaling are associated with brain lamination defects such as lissencephaly, but also with neuropsychiatric diseases like autism, schizophrenia and depression as well with neurodegeneration. Reelin signaling involves a core pathway that activates upon reelin binding to its receptors, particularly ApoER2 (apolipoprotein E receptor 2)/LRP8 (low-density lipoprotein receptor-related protein 8) and very low-density lipoprotein receptor, followed by Src/Fyn-mediated phosphorylation of the adaptor protein Dab1 (Disabled-1). Phosphorylated Dab1 (pDab1) is a hub in the signaling cascade, from which several other downstream pathways diverge reflecting the different roles of reelin. Many of these pathways affect the dynamics of the actin and microtubular cytoskeleton, as well as membrane trafficking through the regulation of the activity of small GTPases, including the Rho and Rap families and molecules involved in cell polarity. The complexity of reelin functions is reflected by the fact that, even now, the precise mode of action of this signaling cascade in vivo at the cellular and molecular levels remains unclear. This review addresses and discusses in detail the participation of reelin in the processes underlying neurogenesis, neuronal migration in the cerebral cortex and the hippocampus; and the polarization, differentiation and maturation processes that neurons experiment in order to be functional in the adult brain. In vivo and in vitro evidence is presented in order to facilitate a better understanding of this fascinating system. © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

  18. Protein kinase A-regulated membrane trafficking of a green fluorescent protein-aquaporin 5 chimera in MDCK cells.

    PubMed

    Kosugi-Tanaka, Chisato; Li, Xuefei; Yao, Chenjuan; Akamatsu, Tetsuya; Kanamori, Norio; Hosoi, Kazuo

    2006-04-01

    The green fluorescent protein (GFP) of the jellyfish, Aeqorea victoria, was used as an autofluorescent tag to track the trafficking of aquaporin 5 (AQP5), an exocrine gland-type water channel. Two groups of chimeric proteins were constructed; one in which GFP was fused to the amino-terminus of AQP5 (GFP-AQP5) and the other, in which it was fused to the carboxyl terminus of it (AQP5-GFP). In each group, 2 chimeras were produced, a wild-type AQP5 with its normal sequence and a mutant AQP5 having a mutated amino acid at 259, i.e., GFP-AQP5-T259A and AQP5-GFP-T259A. They were used to transfect Madin-Darby canine kidney (MDCK) cells. The GFP-AQP5 chimera was localized in the intracellular vesicles, which trafficked to the plasma membrane in response to N(6), 2'-O-dibutyryladenosine 3', 5'-cyclic monophosphate (dbcAMP). Membrane trafficking was inhibited by N-[2-(p-bromocinnamylamino)ethyl]-5-isoquimolinesulfonamide (H-89) but not by palmitoyl-dl-carnitine chloride (PCC). In contrast, the AQP5-GFP chimera expressed in MDCK cells was localized constitutively on the plasma membrane. The cellular localization of the latter chimera was not affected by stimulation with dbcAMP in the presence or absence of H-89 or PCC. Replacement of Thr-259 with Ala-259 did not affect the dbcAMP-induced translocation of the chimeric protein, suggesting that phosphorylation of Thr-259 was not necessary for AQP5 trafficking under the present experimental conditions. Thus, the GFP-AQP5 chimera will be a useful tool to study AQP5 trafficking in vitro, whereas the constitutive membrane localization of the AQP5-GFP chimera suggests the importance of the carboxyl terminus of the AQP5 protein for its sorting, whether it is translocated to intracellular vesicles or to the plasma membrane.

  19. Increased ubiquitination and reduced plasma membrane trafficking of placental amino acid transporter SNAT-2 in human IUGR.

    PubMed

    Chen, Yi-Yung; Rosario, Fredrick J; Shehab, Majida Abu; Powell, Theresa L; Gupta, Madhulika B; Jansson, Thomas

    2015-12-01

    Placental amino acid transport is decreased in intrauterine growth restriction (IUGR); however, the underlying mechanisms remain largely unknown. We have shown that mechanistic target of rapamycin (mTOR) signalling regulates system A amino acid transport by modulating the ubiquitination and plasma membrane trafficking of sodium-coupled neutral amino acid transporter 2 (SNAT-2) in cultured primary human trophoblast cells. We hypothesize that IUGR is associated with (1) inhibition of placental mTORC1 and mTORC2 signalling pathways, (2) increased amino acid transporter ubiquitination in placental homogenates and (3) decreased protein expression of SNAT-2 in the syncytiotrophoblast microvillous plasma membrane (MVM). To test this hypothesis, we collected placental tissue and isolated MVM from women with pregnancies complicated by IUGR (n=25) and gestational age-matched women with appropriately grown control infants (n=19, birth weights between the twenty-fifth to seventy-fifth percentiles). The activity of mTORC1 and mTORC2 was decreased whereas the protein expression of the ubiquitin ligase NEDD4-2 (neural precursor cell expressed developmentally down-regulated protein 4-2; +72%, P<0.0001) and the ubiquitination of SNAT-2 (+180%, P<0.05) were increased in homogenates of IUGR placentas. Furthermore, IUGR was associated with decreased system A amino acid transport activity (-72%, P<0.0001) and SNAT-1 (-42%, P<0.05) and SNAT-2 (-31%, P<0.05) protein expression in MVM. In summary, these findings are consistent with the possibility that decreased placental mTOR activity causes down-regulation of placental system A activity by shifting SNAT-2 trafficking towards proteasomal degradation, thereby contributing to decreased fetal amino acid availability and restricted fetal growth in IUGR. © 2015 Authors; published by Portland Press Limited.

  20. Benzoic Acid, a Weak Organic Acid Food Preservative, Exerts Specific Effects on Intracellular Membrane Trafficking Pathways in Saccharomyces cerevisiae

    PubMed Central

    Hazan, Reut; Levine, Alexandra; Abeliovich, Hagai

    2004-01-01

    Microbial spoilage of food causes losses of up to 40% of all food grown for human consumption worldwide. Yeast growth is a major factor in the spoilage of foods and beverages that are characterized by a high sugar content, low pH, and low water activity, and it is a significant economic problem. While growth of spoilage yeasts such as Zygosaccharomyces bailii and Saccharomyces cerevisiae can usually be retarded by weak organic acid preservatives, the inhibition often requires levels of preservative that are near or greater than the legal limits. We identified a novel synergistic effect of the chemical preservative benzoic acid and nitrogen starvation: while exposure of S. cerevisiae to either benzoic acid or nitrogen starvation is cytostatic under our conditions, the combination of the two treatments is cytocidal and can therefore be used beneficially in food preservation. In yeast, as in all eukaryotic organisms, survival under nitrogen starvation conditions requires a cellular response called macroautophagy. During macroautophagy, cytosolic material is sequestered by intracellular membranes. This material is then targeted for lysosomal degradation and recycled into molecular building blocks, such as amino acids and nucleotides. Macroautophagy is thought to allow cellular physiology to continue in the absence of external resources. Our analyses of the effects of benzoic acid on intracellular membrane trafficking revealed that there was specific inhibition of macroautophagy. The data suggest that the synergism between nitrogen starvation and benzoic acid is the result of inhibition of macroautophagy by benzoic acid and that a mechanistic understanding of this inhibition should be beneficial in the development of novel food preservation technologies. PMID:15294772

  1. Increased ubiquitination and reduced plasma membrane trafficking of placental amino acid transporter SNAT-2 in human IUGR

    PubMed Central

    Rosario, Fredrick J.; Shehab, Majida Abu; Powell, Theresa L.; Gupta, Madhulika B.; Jansson, Thomas

    2015-01-01

    Placental amino acid transport is decreased in intrauterine growth restriction (IUGR); however, the underlying mechanisms remain largely unknown. We have shown that mechanistic target of rapamycin (mTOR) signalling regulates system A amino acid transport by modulating the ubiquitination and plasma membrane trafficking of sodium-coupled neutral amino acid transporter 2 (SNAT-2) in cultured primary human trophoblast cells. We hypothesize that IUGR is associated with (1) inhibition of placental mTORC1 and mTORC2 signalling pathways, (2) increased amino acid transporter ubiquitination in placental homogenates and (3) decreased protein expression of SNAT-2 in the syncytiotrophoblast microvillous plasma membrane (MVM). To test this hypothesis, we collected placental tissue and isolated MVM from women with pregnancies complicated by IUGR (n=25) and gestational age-matched women with appropriately grown control infants (n=19, birth weights between the twenty-fifth to seventy-fifth percentiles). The activity of mTORC1 and mTORC2 was decreased whereas the protein expression of the ubiquitin ligase NEDD4-2 (neural precursor cell expressed developmentally down-regulated protein 4-2; +72%, P<0.0001) and the ubiquitination of SNAT-2 (+180%, P<0.05) were increased in homogenates of IUGR placentas. Furthermore, IUGR was associated with decreased system A amino acid transport activity (–72%, P<0.0001) and SNAT-1 (–42%, P<0.05) and SNAT-2 (–31%, P<0.05) protein expression in MVM. In summary, these findings are consistent with the possibility that decreased placental mTOR activity causes down-regulation of placental system A activity by shifting SNAT-2 trafficking towards proteasomal degradation, thereby contributing to decreased fetal amino acid availability and restricted fetal growth in IUGR. PMID:26374858

  2. Time step rescaling recovers continuous-time dynamical properties for discrete-time Langevin integration of nonequilibrium systems.

    PubMed

    Sivak, David A; Chodera, John D; Crooks, Gavin E

    2014-06-19

    When simulating molecular systems using deterministic equations of motion (e.g., Newtonian dynamics), such equations are generally numerically integrated according to a well-developed set of algorithms that share commonly agreed-upon desirable properties. However, for stochastic equations of motion (e.g., Langevin dynamics), there is still broad disagreement over which integration algorithms are most appropriate. While multiple desiderata have been proposed throughout the literature, consensus on which criteria are important is absent, and no published integration scheme satisfies all desiderata simultaneously. Additional nontrivial complications stem from simulating systems driven out of equilibrium using existing stochastic integration schemes in conjunction with recently developed nonequilibrium fluctuation theorems. Here, we examine a family of discrete time integration schemes for Langevin dynamics, assessing how each member satisfies a variety of desiderata that have been enumerated in prior efforts to construct suitable Langevin integrators. We show that the incorporation of a novel time step rescaling in the deterministic updates of position and velocity can correct a number of dynamical defects in these integrators. Finally, we identify a particular splitting (related to the velocity Verlet discretization) that has essentially universally appropriate properties for the simulation of Langevin dynamics for molecular systems in equilibrium, nonequilibrium, and path sampling contexts.

  3. Time Step Rescaling Recovers Continuous-Time Dynamical Properties for Discrete-Time Langevin Integration of Nonequilibrium Systems

    PubMed Central

    2015-01-01

    When simulating molecular systems using deterministic equations of motion (e.g., Newtonian dynamics), such equations are generally numerically integrated according to a well-developed set of algorithms that share commonly agreed-upon desirable properties. However, for stochastic equations of motion (e.g., Langevin dynamics), there is still broad disagreement over which integration algorithms are most appropriate. While multiple desiderata have been proposed throughout the literature, consensus on which criteria are important is absent, and no published integration scheme satisfies all desiderata simultaneously. Additional nontrivial complications stem from simulating systems driven out of equilibrium using existing stochastic integration schemes in conjunction with recently developed nonequilibrium fluctuation theorems. Here, we examine a family of discrete time integration schemes for Langevin dynamics, assessing how each member satisfies a variety of desiderata that have been enumerated in prior efforts to construct suitable Langevin integrators. We show that the incorporation of a novel time step rescaling in the deterministic updates of position and velocity can correct a number of dynamical defects in these integrators. Finally, we identify a particular splitting (related to the velocity Verlet discretization) that has essentially universally appropriate properties for the simulation of Langevin dynamics for molecular systems in equilibrium, nonequilibrium, and path sampling contexts. PMID:24555448

  4. Pseudo-time stepping methods for space time discontinuous Galerkin discretizations of the compressible Navier Stokes equations

    NASA Astrophysics Data System (ADS)

    Klaij, C. M.; van der Vegt, J. J. W.; van der Ven, H.

    2006-12-01

    The space-time discontinuous Galerkin discretization of the compressible Navier-Stokes equations results in a non-linear system of algebraic equations, which we solve with pseudo-time stepping methods. We show that explicit Runge-Kutta methods developed for the Euler equations suffer from a severe stability constraint linked to the viscous part of the equations and propose an alternative to relieve this constraint while preserving locality. To evaluate its effectiveness, we compare with an implicit-explicit Runge-Kutta method which does not suffer from the viscous stability constraint. We analyze the stability of the methods and illustrate their performance by computing the flow around a 2D airfoil and a 3D delta wing at low and moderate Reynolds numbers.

  5. The rice dynamin-related protein DRP2B mediates membrane trafficking, and thereby plays a critical role in secondary cell wall cellulose biosynthesis.

    PubMed

    Xiong, Guangyan; Li, Rui; Qian, Qian; Song, Xueqin; Liu, Xiangling; Yu, Yanchun; Zeng, Dali; Wan, Jianmin; Li, Jiayang; Zhou, Yihua

    2010-10-01

    Membrane trafficking between the plasma membrane (PM) and intracellular compartments is an important process that regulates the deposition and metabolism of cell wall polysaccharides. Dynamin-related proteins (DRPs), which function in membrane tubulation and vesiculation are closely associated with cell wall biogenesis. However, the molecular mechanisms by which DRPs participate in cell wall formation are poorly understood. Here, we report the functional characterization of Brittle Culm3 (BC3), a gene encoding OsDRP2B. Consistent with the expression of BC3 in mechanical tissues, the bc3 mutation reduces mechanical strength, which results from decreased cellulose content and altered secondary wall structure. OsDRP2B, one of three members of the DRP2 subfamily in rice (Oryza sativa L.), was identified as an authentic membrane-associated dynamin via in vitro biochemical analyses. Subcellular localization of fluorescence-tagged OsDRP2B and several compartment markers in protoplast cells showed that this protein not only lies at the PM and the clathrin-mediated vesicles, but also is targeted to the trans-Golgi network (TGN). An FM4-64 uptake assay in transgenic plants that express green fluorescent protein-tagged OsDRP2B verified its involvement in an endocytic pathway. BC3 mutation and overexpression altered the abundance of cellulose synthase catalytic subunit 4 (OsCESA4) in the PM and in the endomembrane systems. All of these findings lead us to conclude that OsDRP2B participates in the endocytic pathway, probably as well as in post-Golgi membrane trafficking. Mutation of OsDRP2B disturbs the membrane trafficking that is essential for normal cellulose biosynthesis of the secondary cell wall, thereby leading to inferior mechanical properties in rice plants.

  6. The N-terminal region of organic anion transporting polypeptide 1B3 (OATP1B3) plays an essential role in regulating its plasma membrane trafficking.

    PubMed

    Chun, Se-Eun; Thakkar, Nilay; Oh, Yunseok; Park, Ji Eun; Han, Songhee; Ryoo, Gongmi; Hahn, Hyunggu; Maeng, Sang Hyun; Lim, Young-Ran; Han, Byung Woo; Lee, Wooin

    2017-05-01

    Organic anion transporting polypeptide 1B3 (OATP1B3) is a major influx transporter mediating the hepatic uptake of various endogenous substrates as well as clinically important drugs such as statins and anticancer drugs. However, molecular mechanisms controlling the membrane trafficking of OATP1B3 have been largely unknown. Several reports recently indicated the presence of a distinct, cancer-type OATP1B3 variant lacking the N-terminal 28 amino acids compared to OATP1B3 expressed in non-malignant hepatocytes. Interestingly, the cancer-type OATP1B3 variant is located predominantly in the cytoplasm, implicating the involvement of the N-terminal region of OATP1B3 in its membrane trafficking. In the current study, we set out to experimentally validate the importance of the N-terminal region of OATP1B3 and to identify responsible sequence motif(s) in that region. A number of truncation or point mutants of OATP1B3 were transiently expressed in HEK293T, HCT-8 or MDCK II cells and their expression in cytoplasmic and surface membrane fractions were analyzed by immunoblotting. Our results indicated that the N-terminal sequence of OATP1B3, in particular, at the amino acid positions between 12 and 28, may be indispensable in its membrane trafficking. Moreover, our results using a fusion construct indicated that the first 50 amino acids of OATP1B3 are sufficient for its membrane localization. The importance of the N-terminal region in membranous localization was shared among the other OATP1B subfamily members, OATP1B1 and rat Oatp1b2. Our efforts to identify the responsible amino acid(s) or structure motif(s) in the N-terminal region did not pinpoint individual amino acids or motifs with putative secondary structures. Our current findings however demonstrate that the N-terminal region is important for the membrane localization of the OATP1B subfamily members and should facilitate future investigations of the mechanisms involved in the regulation and membrane trafficking of

  7. Fully implicit solutions of the benchmark backward facing step problem using finite element discretization and inexact Newton's method

    SciTech Connect

    McHugh, P.R.; Knoll, D.A.

    1992-01-01

    A fully implicit solution algorithm based on Newton's method is used to solve the steady, incompressible Navier-Stokes and energy equations. An efficiently evaluated numerical Jacobian is used to simplify implementation, and mesh sequencing is used to increase the radius of convergence of the algorithm. We employ finite volume discretization using the power law scheme of Patankar to solve the benchmark backward facing step problem defined by the ASME K-12 Aerospace Heat Transfer Committee. LINPACK banded Gaussian elimination and the preconditioned transpose-free quasi-minimal residual (TFQMR) algorithm of Freund are studied as possible linear equation solvers. Implementation of the preconditioned TFQMR algorithm requires use of the switched evolution relaxation algorithm of Mulder and Van Leer to ensure convergence. The preconditioned TFQMR algorithm is more memory efficient than the direct solver, but our implementation is not as CPU efficient. Results show that for the level of grid refinement used, power law differencing was not adequate to yield the desired accuracy for this problem.

  8. Interaction between small GTPase Rab7 and PI3KC3 links autophagy and endocytosis: A new Rab7 effector protein sheds light on membrane trafficking pathways.

    PubMed

    Lin, Mary Grace; Zhong, Qing

    2011-03-01

    Endocytosis and autophagy are both membrane trafficking pathways vital for cell survival. Endocytosis, the primary means by which cells internalize material such as cell-surface receptors and their protein ligands, is essential for proper cell growth and communication. Autophagy is a catabolic process that degrades cargo ranging from organelles to protein aggregates to bacteria, and it is important for maintaining cellular homeostasis. Defects in both endosome and autophagosome maturation lead to an array of human diseases, including cancer; however, the molecular mechanisms underlying endosome and autophagosome maturation are not well characterized. In the case of endocytosis, small GTPases, key players in membrane organization, are required for endosome maturation. Specifically, activation of the small GTPase Rab7 is required for the initiation of the early-to-late endosome transition, although how this is regulated is largely unknown. Now recent findings from our laboratory show that Rubicon, a component of the PI3KC3 complex, inhibits endosome maturation by preventing activation of Rab7. Not only do our results clarify the molecular link between PI3KC3 and Rab7 function in endosome maturation, they lead us to propose new models for PI3KC3 involvement in membrane trafficking, particularly at the convergence between the endosome and autophagosome pathways.

  9. Small RAB GTPases Regulate Multiple Steps of Mitosis

    PubMed Central

    Miserey-Lenkei, Stéphanie; Colombo, María I.

    2016-01-01

    GTPases of the RAB family are key regulators of multiple steps of membrane trafficking. Several members of the RAB GTPase family have been implicated in mitotic progression. In this review, we will first focus on the function of endosome-associated RAB GTPases reported in early steps of mitosis, spindle pole maturation, and during cytokinesis. Second, we will discuss the role of Golgi-associated RAB GTPases at the metaphase/anaphase transition and during cytokinesis. PMID:26925400

  10. Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus

    PubMed Central

    2010-01-01

    Background Dengue virus (DENV) is the causative agent of Dengue fever and the life-threatening Dengue Haemorrhagic fever or Dengue shock syndrome. In the absence of anti-viral agents or vaccine, there is an urgent need to develop an effective anti-viral strategy against this medically important viral pathogen. The initial interplay between DENV and the host cells may represent one of the potential anti-viral targeting sites. Currently the involvements of human membrane trafficking host genes or factors that mediate the infectious cellular entry of dengue virus are not well defined. Results In this study, we have used a targeted small interfering RNA (siRNA) library to identify and profile key cellular genes involved in processes of endocytosis, cytoskeletal dynamics and endosome trafficking that are important and essential for DENV infection. The infectious entry of DENV into Huh7 cells was shown to be potently inhibited by siRNAs targeting genes associated with clathrin-mediated endocytosis. The important role of clathrin-mediated endocytosis was confirmed by the expression of well-characterized dominant-negative mutants of genes in this pathway and by using the clathrin endocytosis inhibitor chlorpromazine. Furthermore, DENV infection was shown to be sensitive to the disruption of human genes in regulating the early to late endosomal trafficking as well as the endosomal acidic pH. The importance and involvement of both actin and microtubule dynamics in mediating the infectious entry of DENV was also revealed in this study. Conclusions Together, the findings from this study have provided a detail profiling of the human membrane trafficking cellular genes and the mechanistic insight into the interplay of these host genes with DENV to initiate an infection, hence broadening our understanding on the entry pathway of this medically important viral pathogen. These data may also provide a new potential avenue for development of anti-viral strategies and treatment of

  11. Step length after discrete perturbation predicts accidental falls and fall-related injury in elderly people with a range of peripheral neuropathy.

    PubMed

    Allet, Lara; Kim, Hogene; Ashton-Miller, James; De Mott, Trina; Richardson, James K

    2014-01-01

    Distal symmetric polyneuropathy increases fall risk due to inability to cope with perturbations. We aimed to 1) identify the frontal plane lower limb sensorimotor functions which are necessary for robustness to a discrete, underfoot perturbation during gait; and 2) determine whether changes in the post-perturbed step parameters could distinguish between fallers and non fallers. Forty-two subjects (16 healthy old and 26 with diabetic PN) participated. Frontal plane lower limb sensorimotor functions were determined using established laboratory-based techniques. The subjects' most extreme alterations in step width or step length in response to a perturbation were measured. In addition, falls and fall-related injuries were prospectively recorded. Ankle proprioceptive threshold (APrT; p=.025) and hip abduction rate of torque generation (RTG; p=.041) independently predicted extreme step length after medial perturbation, with precise APrT and greater hip RTG allowing maintenance of step length. Injured subjects demonstrated greater extreme step length changes after medial perturbation than non-injured subjects (percent change = 18.5 ± 9.2 vs. 11.3 ± 4.57; p = .01). The ability to rapidly generate frontal plane hip strength and/or precisely perceive motion at the ankle is needed to maintain a normal step length after perturbation, a parameter which distinguishes between subjects sustaining a fall-related injury and those who did not. © 2014.

  12. Exendin-4 promotes the membrane trafficking of the AMPA receptor GluR1 subunit and ADAM10 in the mouse neocortex.

    PubMed

    Ohtake, Nobuaki; Saito, Mieko; Eto, Masaaki; Seki, Kenjiro

    2014-05-01

    Glucagon-like peptide-1 (GLP-1) is a novel treatment modality for type 2 diabetes mellitus. However, GLP-1 has been suggested as a therapeutic target for Alzheimer's disease (AD). In rodent studies, GLP-1 reduces amyloid beta (Aβ) and facilitates synaptic plasticity. Therefore, in the present study, we investigated how GLP-1 facilitates synaptic plasticity and reduces the Aβ in vivo. Exendin-4, a GLP-1 receptor agonist that can cross the blood brain barrier, was subcutaneously administered to adult mice. We then extracted the total and the plasma membrane proteins from the mouse neocortex. Exendin-4 significantly increased the phosphorylation level of cAMP response element-binding protein (CREB). Consistently, the expression level of brain-derived neurotrophic factor (BDNF), a transcriptional target of CREB, was increased. Furthermore, exendin-4 increased the membrane protein level of the AMPA receptor GluR1 subunit and postsynaptic density protein-95 (PSD-95), whereas GluR2 was unaffected. These exendin-4-dependent increases in membrane GluR1, total PSD-95 and BDNF were abrogated by pretreatment with temozolomide (TMZ), a DNA-alkylating agent, indicating that these alterations were dependent on exendin-4-induced transcriptional activity. In addition, we found that exendin-4 increased the level of the α-C terminal fragment (α-CTF) of amyloid precursor protein (APP). Furthermore, protein levels of both mature and immature ADAM10, the α-secretase of APP in the plasma membrane, were increased, whereas the total mature and immature ADAM10 levels were unchanged. These exendin-4-dependent increases in α-CTF and ADAM10 were not affected by TMZ. These findings suggested that GLP-1 facilitates the GluR1 membrane insertion through CREB activation and increases α-secretase activity through ADAM10 membrane trafficking. Upregulation of GluR1 and ADAM10 at the plasma membrane were also observed in mice with intracerebroventricular administration of Aβ oligomer

  13. Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis.

    PubMed

    Naramoto, Satoshi; Otegui, Marisa S; Kutsuna, Natsumaro; de Rycke, Riet; Dainobu, Tomoko; Karampelias, Michael; Fujimoto, Masaru; Feraru, Elena; Miki, Daisuke; Fukuda, Hiroo; Nakano, Akihiko; Friml, Jiří

    2014-07-01

    GNOM is one of the most characterized membrane trafficking regulators in plants, with crucial roles in development. GNOM encodes an ARF-guanine nucleotide exchange factor (ARF-GEF) that activates small GTPases of the ARF (ADP ribosylation factor) class to mediate vesicle budding at endomembranes. The crucial role of GNOM in recycling of PIN auxin transporters and other proteins to the plasma membrane was identified in studies using the ARF-GEF inhibitor brefeldin A (BFA). GNOM, the most prominent regulator of recycling in plants, has been proposed to act and localize at so far elusive recycling endosomes. Here, we report the GNOM localization in context of its cellular function in Arabidopsis thaliana. State-of-the-art imaging, pharmacological interference, and ultrastructure analysis show that GNOM predominantly localizes to Golgi apparatus. Super-resolution confocal live imaging microscopy identified GNOM and its closest homolog GNOM-like 1 at distinct subdomains on Golgi cisternae. Short-term BFA treatment stabilizes GNOM at the Golgi apparatus, whereas prolonged exposures results in GNOM translocation to trans-Golgi network (TGN)/early endosomes (EEs). Malformed TGN/EE in gnom mutants suggests a role for GNOM in maintaining TGN/EE function. Our results redefine the subcellular action of GNOM and reevaluate the identity and function of recycling endosomes in plants.

  14. C-Terminal Di-leucine Motif of Dopamine D1 Receptor Plays an Important Role in Its Plasma Membrane Trafficking

    PubMed Central

    Guo, Yan; Jose, Pedro A.

    2011-01-01

    The dopamine D1 receptor (D1R), a G protein-coupled receptor, plays a critical role in regulating blood pressure through its actions on renal hemodynamics and epithelial ion transport, which are highly linked to its intracellular trafficking. In this study, we generated a series of C-terminal mutants of D1R that were tagged with or without enhanced yellow fluorescent protein, and analyzed the consequences of these mutants on the plasma membrane trafficking of D1R and cyclic AMP response to D1R stimulation. D1R with mutations within the endocytic recycling signal (amino acid residues 360–382) continued to be functional, albeit decreased relative to wild-type D1R. Mutation of the palmitoylation site (347C>S) of D1R did not impair its trafficking to the plasma membrane, but abolished its ability to increase cyclic AMP accumulation. In contrast, replacement of di-leucines (344–345L>A) by alanines resulted in the retention of D1R in the early endosome, decreased its glycosylation, and prevented its targeting to the plasma membrane. Our studies suggest that di-L motif at the C-terminus of D1R is critical for the glycosylation and cell surface targeting of D1R. PMID:22206002

  15. Sequestration of AS-DACA into acidic compartments of the membrane trafficking system as a mechanism of drug resistance in rhabdomyosarcoma.

    PubMed

    Williams, Marissa; Catchpoole, Daniel

    2013-06-25

    The accumulation of weakly basic drugs into acidic organelles has recently been described as a contributor to resistance in childhood cancer rhabdomyosarcoma (RMS) cell lines with differential sensitivity to a novel topoisomerase II inhibitor, AS-DACA. The current study aims to explore the contribution of the endocytic pathway to AS-DACA sequestration in RMS cell lines. A 24-fold differential in AS-DACA cytotoxicity was detected between the RMS lines RD and Rh30. The effect of inhibitors of the endocytic pathway on AS-DACA sensitivity in RMS cell lines, coupled with the variations of endosomal marker expression, indicated the late endosomal/lysosomal compartment was implicated by confounding lines of evidence. Higher expression levels of Lysosomal-Associated Membrane Protein-1 (LAMP1) in the resistant RMS cell line, RD, provided correlations between the increased amount and activity of these compartments to AS-DACA resistance. The late endosomal inhibitor 3-methyladenine increased AS-DACA sensitivity solely in RD leading to the reduction of AS-DACA in membrane trafficking organelles. Acidification inhibitors did not produce an increase in AS-DACA sensitivity nor reduce its sequestration, indicating that the pH partitioning of weakly basic drugs into acidic compartments does not likely contribute to the AS-DACA sequestering resistance mechanism evident in RMS cells.

  16. Sequestration of AS-DACA into Acidic Compartments of the Membrane Trafficking System as a Mechanism of Drug Resistance in Rhabdomyosarcoma

    PubMed Central

    Williams, Marissa; Catchpoole, Daniel

    2013-01-01

    The accumulation of weakly basic drugs into acidic organelles has recently been described as a contributor to resistance in childhood cancer rhabdomyosarcoma (RMS) cell lines with differential sensitivity to a novel topoisomerase II inhibitor, AS-DACA. The current study aims to explore the contribution of the endocytic pathway to AS-DACA sequestration in RMS cell lines. A 24-fold differential in AS-DACA cytotoxicity was detected between the RMS lines RD and Rh30. The effect of inhibitors of the endocytic pathway on AS-DACA sensitivity in RMS cell lines, coupled with the variations of endosomal marker expression, indicated the late endosomal/lysosomal compartment was implicated by confounding lines of evidence. Higher expression levels of Lysosomal-Associated Membrane Protein-1 (LAMP1) in the resistant RMS cell line, RD, provided correlations between the increased amount and activity of these compartments to AS-DACA resistance. The late endosomal inhibitor 3-methyladenine increased AS-DACA sensitivity solely in RD leading to the reduction of AS-DACA in membrane trafficking organelles. Acidification inhibitors did not produce an increase in AS-DACA sensitivity nor reduce its sequestration, indicating that the pH partitioning of weakly basic drugs into acidic compartments does not likely contribute to the AS-DACA sequestering resistance mechanism evident in RMS cells. PMID:23799359

  17. Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin dynamics and membrane trafficking during Caenorhabditis elegans oocyte growth and embryonic epidermal morphogenesis.

    PubMed

    Giuliani, Chiara; Troglio, Flavia; Bai, Zhiyong; Patel, Falshruti B; Zucconi, Adriana; Malabarba, Maria Grazia; Disanza, Andrea; Stradal, Theresia B; Cassata, Giuseppe; Confalonieri, Stefano; Hardin, Jeffrey D; Soto, Martha C; Grant, Barth D; Scita, Giorgio

    2009-10-01

    The TOCA family of F-BAR-containing proteins bind to and remodel lipid bilayers via their conserved F-BAR domains, and regulate actin dynamics via their N-Wasp binding SH3 domains. Thus, these proteins are predicted to play a pivotal role in coordinating membrane traffic with actin dynamics during cell migration and tissue morphogenesis. By combining genetic analysis in Caenorhabditis elegans with cellular biochemical experiments in mammalian cells, we showed that: i) loss of CeTOCA proteins reduced the efficiency of Clathrin-mediated endocytosis (CME) in oocytes. Genetic interference with CeTOCAs interacting proteins WSP-1 and WVE-1, and other components of the WVE-1 complex, produced a similar effect. Oocyte endocytosis defects correlated well with reduced egg production in these mutants. ii) CeTOCA proteins localize to cell-cell junctions and are required for proper embryonic morphogenesis, to position hypodermal cells and to organize junctional actin and the junction-associated protein AJM-1. iii) Double mutant analysis indicated that the toca genes act in the same pathway as the nematode homologue of N-WASP/WASP, wsp-1. Furthermore, mammalian TOCA-1 and C. elegans CeTOCAs physically associated with N-WASP and WSP-1 directly, or WAVE2 indirectly via ABI-1. Thus, we propose that TOCA proteins control tissues morphogenesis by coordinating Clathrin-dependent membrane trafficking with WAVE and N-WASP-dependent actin-dynamics.

  18. Requirements for F-BAR Proteins TOCA-1 and TOCA-2 in Actin Dynamics and Membrane Trafficking during Caenorhabditis elegans Oocyte Growth and Embryonic Epidermal Morphogenesis

    PubMed Central

    Patel, Falshruti B.; Zucconi, Adriana; Malabarba, Maria Grazia; Disanza, Andrea; Stradal, Theresia B.; Cassata, Giuseppe; Confalonieri, Stefano; Hardin, Jeffrey D.; Soto, Martha C.; Grant, Barth D.; Scita, Giorgio

    2009-01-01

    The TOCA family of F-BAR–containing proteins bind to and remodel lipid bilayers via their conserved F-BAR domains, and regulate actin dynamics via their N-Wasp binding SH3 domains. Thus, these proteins are predicted to play a pivotal role in coordinating membrane traffic with actin dynamics during cell migration and tissue morphogenesis. By combining genetic analysis in Caenorhabditis elegans with cellular biochemical experiments in mammalian cells, we showed that: i) loss of CeTOCA proteins reduced the efficiency of Clathrin-mediated endocytosis (CME) in oocytes. Genetic interference with CeTOCAs interacting proteins WSP-1 and WVE-1, and other components of the WVE-1 complex, produced a similar effect. Oocyte endocytosis defects correlated well with reduced egg production in these mutants. ii) CeTOCA proteins localize to cell–cell junctions and are required for proper embryonic morphogenesis, to position hypodermal cells and to organize junctional actin and the junction-associated protein AJM-1. iii) Double mutant analysis indicated that the toca genes act in the same pathway as the nematode homologue of N-WASP/WASP, wsp-1. Furthermore, mammalian TOCA-1 and C. elegans CeTOCAs physically associated with N-WASP and WSP-1 directly, or WAVE2 indirectly via ABI-1. Thus, we propose that TOCA proteins control tissues morphogenesis by coordinating Clathrin-dependent membrane trafficking with WAVE and N-WASP–dependent actin-dynamics. PMID:19798448

  19. Rice OsVAMP714, a membrane-trafficking protein localized to the chloroplast and vacuolar membrane, is involved in resistance to rice blast disease.

    PubMed

    Sugano, Shoji; Hayashi, Nagao; Kawagoe, Yasushi; Mochizuki, Susumu; Inoue, Haruhiko; Mori, Masaki; Nishizawa, Yoko; Jiang, Chang-Jie; Matsui, Minami; Takatsuji, Hiroshi

    2016-05-01

    Membrane trafficking plays pivotal roles in many cellular processes including plant immunity. Here, we report the characterization of OsVAMP714, an intracellular SNARE protein, focusing on its role in resistance to rice blast disease caused by the fungal pathogen Magnaporthe oryzae. Disease resistance tests using OsVAMP714 knockdown and overexpressing rice plants demonstrated the involvement of OsVAMP714 in blast resistance. The overexpression of OsVAMP7111, whose product is highly homologous to OsVAMP714, did not enhance blast resistance to rice, implying a potential specificity of OsVAMP714 to blast resistance. OsVAMP714 was localized to the chloroplast in mesophyll cells and to the cellular periphery in epidermal cells of transgenic rice plant leaves. We showed that chloroplast localization is critical for the normal OsVAMP714 functioning in blast resistance by analyzing the rice plants overexpressing OsVAMP714 mutants whose products did not localize in the chloroplast. We also found that OsVAMP714 was located in the vacuolar membrane surrounding the invasive hyphae of M. oryzae. Furthermore, we showed that OsVAMP714 overexpression promotes leaf sheath elongation and that the first 19 amino acids, which are highly conserved between animal and plant VAMP7 proteins, are crucial for normal rice plant growths. Our studies imply that the OsVAMP714-mediated trafficking pathway plays an important role in rice blast resistance as well as in the vegetative growth of rice.

  20. Probing the Membrane Environment of the TOR Kinases Reveals Functional Interactions between TORC1, Actin, and Membrane Trafficking in Saccharomyces cerevisiae

    PubMed Central

    Aronova, Sofia; Wedaman, Karen; Anderson, Scott; Yates, John

    2007-01-01

    The TOR kinases are regulators of growth in eukaryotic cells that assemble into two distinct protein complexes, TORC1 and TORC2, where TORC1 is inhibited by the antibiotic rapamycin. Present models favor a view wherein TORC1 regulates cell mass accumulation, and TORC2 regulates spatial aspects of growth, including organization of the actin cytoskeleton. Here, we demonstrate that in yeast both TORC1 and TORC2 fractionate with a novel form of detergent-resistant membranes that are distinct from detergent-resistant plasma membrane “rafts.” Proteomic analysis of these TOR-associated membranes revealed the presence of regulators of endocytosis and the actin cytoskeleton. Genetic analyses revealed a significant number of interactions between these components and TORC1, demonstrating a functional link between TORC1 and actin/endocytosis-related genes. Moreover, we found that inhibition of TORC1 by rapamycin 1) disrupted actin polarization, 2) delayed actin repolarization after glucose starvation, and 3) delayed accumulation of lucifer yellow within the vacuole. By combining our genetic results with database mining, we constructed a map of interactions that led to the identification of additional genetic interactions between TORC1 and components involved in membrane trafficking. Together, these results reveal the broad scope of cellular processes influenced by TORC1, and they underscore the functional overlap between TORC1 and TORC2. PMID:17507646

  1. Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis[W

    PubMed Central

    Naramoto, Satoshi; Otegui, Marisa S.; Kutsuna, Natsumaro; de Rycke, Riet; Dainobu, Tomoko; Karampelias, Michael; Fujimoto, Masaru; Feraru, Elena; Miki, Daisuke; Fukuda, Hiroo; Nakano, Akihiko; Friml, Jiří

    2014-01-01

    GNOM is one of the most characterized membrane trafficking regulators in plants, with crucial roles in development. GNOM encodes an ARF-guanine nucleotide exchange factor (ARF-GEF) that activates small GTPases of the ARF (ADP ribosylation factor) class to mediate vesicle budding at endomembranes. The crucial role of GNOM in recycling of PIN auxin transporters and other proteins to the plasma membrane was identified in studies using the ARF-GEF inhibitor brefeldin A (BFA). GNOM, the most prominent regulator of recycling in plants, has been proposed to act and localize at so far elusive recycling endosomes. Here, we report the GNOM localization in context of its cellular function in Arabidopsis thaliana. State-of-the-art imaging, pharmacological interference, and ultrastructure analysis show that GNOM predominantly localizes to Golgi apparatus. Super-resolution confocal live imaging microscopy identified GNOM and its closest homolog GNOM-like 1 at distinct subdomains on Golgi cisternae. Short-term BFA treatment stabilizes GNOM at the Golgi apparatus, whereas prolonged exposures results in GNOM translocation to trans-Golgi network (TGN)/early endosomes (EEs). Malformed TGN/EE in gnom mutants suggests a role for GNOM in maintaining TGN/EE function. Our results redefine the subcellular action of GNOM and reevaluate the identity and function of recycling endosomes in plants. PMID:25012191

  2. A novel tribasic Golgi export signal directs cargo protein interaction with activated Rab11 and AP-1-dependent Golgi-plasma membrane trafficking.

    PubMed

    Parmar, Hirendrasinh B; Duncan, Roy

    2016-04-15

    The reovirus fusion-associated small transmembrane (FAST) proteins comprise a unique family of viral membrane fusion proteins dedicated to inducing cell-cell fusion. We recently reported that a polybasic motif (PBM) in the cytosolic tail of reptilian reovirus p14 FAST protein functions as a novel tribasic Golgi export signal. Using coimmunoprecipitation and fluorescence resonance energy transfer (FRET) assays, we now show the PBM directs interaction of p14 with GTP-Rab11. Overexpression of dominant-negative Rab11 and RNA interference knockdown of endogenous Rab11 inhibited p14 plasma membrane trafficking and resulted in p14 accumulation in the Golgi complex. This is the first example of Golgi export to the plasma membrane that is dependent on the interaction of membrane protein cargo with activated Rab11. RNA interference and immunofluorescence microscopy further revealed that p14 Golgi export is dependent on AP-1 (but not AP-3 or AP-4) and that Rab11 and AP-1 both colocalize with p14 at the TGN. Together these results imply the PBM mediates interactions of p14 with activated Rab11 at the TGN, resulting in p14 sorting into AP1-coated vesicles for anterograde TGN-plasma membrane transport.

  3. Hybrid High-Fidelity Modeling of Radar Scenarios Using Atemporal, Discrete-Event, and Time-Step Simulation

    DTIC Science & Technology

    2016-12-01

    significant predictors in themeta-model since themodel containing the new predictor statistically performs differently than themodel without the new...degree and four-degree interaction predictors fit model perform quite consistently in RMSE and prediction error rates from the confusion matrix analysis...TIME-STEP SIMULATION by Yuan-Pin Cheng December 2016 Dissertation Supervisors: Donald P. Brutzman Phillip E. Pace This dissertation was performed at the

  4. Optimisation of flux calculation in rivers from discrete water quality surveys, a step towards an expert system

    NASA Astrophysics Data System (ADS)

    Raymond, S.; Moatar, F.; Meybeck, M.; Bustillo, V.

    2009-04-01

    Good estimates of fluxes of suspended particulate matter (SPM), total dissolved solids (TDS) and nutrients and contaminants are required for both Earth System science and river basin management. However, in most cases discrete sampling (weekly to monthly) is the rule. Few flux calculation methods are commonly used, yet their performances, i.e. uncertainties for given frequencies, at given stations and for each water quality variables, remain unknown. Based on a rare set of 1085 station-year of daily flux record for SPM, TDS and nutrients (dissolved and total), the performance of 9 calculations methods is explored. Discrete surveys at various frequencies (3days to 30 days) are simulated by Monte-Carlo sorting (100 runs) on which the 9 fluxes are calculated (annual and interannual). At this stage, the sub-daily variations of fluxes for the medium and large basins are not considered. The dataset for SPM corresponds to 55 stations (600 to 600 000 km2 basin area), 34 stations (700 to 1000000 km2) for TDS and for nutrients we consider 9 stations for NO3-, NH4+, PO43- and Ptot (600 to 30 000 km2). About 80% of the dataset originates from US records (USGS and Lake Erie tributaries survey) and 20% from French stations, this covering a wide range of hydrological and geochemical conditions in the temperate zone. Each sorted flux is compared to known fluxes established on daily records: percentiles of their relative errors (e10, e50 and e90) are used to determine the biases (e50) and the imprecisions (e90-e10) (Walling and Webb, 1981) which are then compared for each of the 6 water quality variables, for each flux methods and for various simulated survey frequencies. The calculation methods include 5 rating-curve approaches (linear"M1", "M2", Phillipps et al, 1999) with and without Ferguson correction (Ferguson, 1987), polynomial, truncated at discharges exceeding median annual or long-term water discharge), 2 methods based on hydrograph separation (Phillips et al, 1999

  5. Extrasynaptic membrane trafficking regulated by GluR1 serine 845 phosphorylation primes AMPA receptors for long-term potentiation.

    PubMed

    Oh, Michael C; Derkach, Victor A; Guire, Eric S; Soderling, Thomas R

    2006-01-13

    Enhancement of synaptic transmission, as occurs in long-term potentiation (LTP), can result from several mechanisms that are regulated by phosphorylation of the AMPA-type glutamate receptor (AMPAR). Using a quantitative assay of net serine 845 (Ser-845) phosphorylation in the GluR1 subunit of AMPARs, we investigated the relationship between phospho-Ser-845, GluR1 surface expression, and synaptic strength in hippocampal neurons. About 15% of surface AMPARs in cultured neurons were phosphorylated at Ser-845 basally, whereas chemical potentiation (forskolin/rolipram treatment) persistently increased this to 60% and chemical depression (N-methyl-D-aspartate treatment) decreased it to 10%. These changes in Ser-845 phosphorylation were paralleled by corresponding changes in the surface expression of AMPARs in both cultured neurons and hippocampal slices. For every 1% increase in net phospho-Ser-845, there was 0.75% increase in the surface fraction of GluR1. Phosphorylation of Ser-845 correlated with a selective delivery of AMPARs to extrasynaptic sites, and their synaptic localization required coincident synaptic activity. Furthermore, increasing the extrasynaptic pool of AMPA receptors resulted in stronger theta burst LTP. Our results support a two-step model for delivery of GluR1-containing AMPARs to synapses during activity-dependent LTP, where Ser-845 phosphorylation can traffic AMPARs to extrasynaptic sites for subsequent delivery to synapses during LTP.

  6. par-1, Atypical pkc, and PP2A/B55 sur-6 Are Implicated in the Regulation of Exocyst-Mediated Membrane Trafficking in Caenorhabditis elegans

    PubMed Central

    Jiu, Yaming; Hasygar, Kiran; Tang, Lois; Liu, Yanbo; Holmberg, Carina I.; Bürglin, Thomas R.; Hietakangas, Ville; Jäntti, Jussi

    2013-01-01

    The exocyst is a conserved protein complex that is involved in tethering secretory vesicles to the plasma membrane and regulating cell polarity. Despite a large body of work, little is known how exocyst function is controlled. To identify regulators for exocyst function, we performed a targeted RNA interference (RNAi) screen in Caenorhabditis elegans to uncover kinases and phosphatases that genetically interact with the exocyst. We identified seven kinase and seven phosphatase genes that display enhanced phenotypes when combined with hypomorphic alleles of exoc-7 (exo70), exoc-8 (exo84), or an exoc-7;exoc-8 double mutant. We show that in line with its reported role in exocytotic membrane trafficking, a defective exoc-8 caused accumulation of exocytotic soluble NSF attachment protein receptor (SNARE) proteins in both intestinal and neuronal cells in C. elegans. Down-regulation of the phosphatase protein phosphatase 2A (PP2A) phosphatase regulatory subunit sur-6/B55 gene resulted in accumulation of exocytic SNARE proteins SNB-1 and SNAP-29 in wild-type and in exoc-8 mutant animals. In contrast, RNAi of the kinase par-1 caused reduced intracellular green fluorescent protein signal for the same proteins. Double RNAi experiments for par-1, pkc-3, and sur-6/B55 in C. elegans suggest a possible cooperation and involvement in postembryo lethality, developmental timing, as well as SNARE protein trafficking. Functional analysis of the homologous kinases and phosphatases in Drosophila median neurosecretory cells showed that atypical protein kinase C kinase and phosphatase PP2A regulate exocyst-dependent, insulin-like peptide secretion. Collectively, these results characterize kinases and phosphatases implicated in the regulation of exocyst function, and suggest the possibility for interplay between the par-1 and pkc-3 kinases and the PP2A phosphatase regulatory subunit sur-6 in this process. PMID:24192838

  7. Ultra-fast formation control of high-order discrete-time multi-agent systems based on multi-step predictive mechanism.

    PubMed

    Zhang, Wenle; Liu, Jianchang; Wang, Honghai

    2015-09-01

    This paper deals with the ultra-fast formation control problem of high-order discrete-time multi-agent systems. Using the local neighbor-error knowledge, a novel ultra-fast protocol with multi-step predictive information and self-feedback term is proposed. The asymptotic convergence factor is improved by a power of q+1 compared to the routine protocol. To some extent, the ultra-fast algorithm overcomes the influence of communication topology to the convergence speed. Furthermore, some sufficient conditions are given herein. The ones decouple the design of the synchronizing gains from the detailed graph properties, and explicitly reveal how the agent dynamic and the communication graph jointly affect the ultra-fast formationability. Finally, some simulations are worked out to illustrate the effectiveness of our theoretical results.

  8. Epigenetic silencing of the c-fms locus during B-lymphopoiesis occurs in discrete steps and is reversible

    PubMed Central

    Tagoh, Hiromi; Schebesta, Alexandra; Lefevre, Pascal; Wilson, Nicola; Hume, David; Busslinger, Meinrad; Bonifer, Constanze

    2004-01-01

    The murine c-fms (Csf1r) gene encodes the macrophage colony-stimulating factor receptor, which is essential for macrophage development. It is expressed at a low level in haematopoietic stem cells and is switched off in all non-macrophage cell types. To examine the role of chromatin structure in this process we studied epigenetic silencing of c-fms during B-lymphopoiesis. c-fms chromatin in stem cells and multipotent progenitors is in the active conformation and bound by transcription factors. A similar result was obtained with specified common myeloid and lymphoid progenitor cells. In developing B cells, c-fms chromatin is silenced in distinct steps, whereby first the binding of transcription factors and RNA expression is lost, followed by a loss of nuclease accessibility. Interestingly, regions of de novo DNA methylation in B cells overlap with an intronic antisense transcription unit that is differently regulated during lymphopoiesis. However, even at mature B cell stages, c-fms chromatin is still in a poised conformation and c-fms expression can be re-activated by conditional deletion of the transcription factor Pax5. PMID:15483629

  9. Excitation energies and spins of a superdeformed band in {sup 194}{ital H}{ital g} from one-step discrete decays to the yrast line

    SciTech Connect

    Khoo, T.L.; Carpenter, M.P.; Lauritsen, T.; Ackermann, D.; Ahmad, I.; Blumenthal, D.J.; Fischer, S.M.; Janssens, R.V.; Nisius, D.; Moore, E.F.; Lopez-Martens, A.; Do Kruecken, R.; Asztalos, S.J.; Becker, J.A.; Bernstein, L.; Clark, R.M.; Deleplanque, M.A.; Diamond, R.M.; Fallon, P.; Farris, L.P.; Hannachi, F.; Henry, E.A.; Korichi, A.; Lee, I.Y.; Macchiavelli, A.O.; Stephens, F.S. ||||||

    1996-03-01

    Discrete {gamma} rays directly connecting states of a superdeformed (SD) band in {sup 194}Hg to the yrast states have been discovered. Thus, the excitation energies and spins of all members of the lowest SD band are established for the first time, together with their likely parity. The SD band decays from its 10{sup +} and 12{sup +} states, which lie 4204.8 and 4407.4 keV above the normal-deformed yrast states of the same spins. {copyright} {ital 1996 The American Physical Society.}

  10. Synchronous Discrete Harmonic Oscillator

    SciTech Connect

    Antippa, Adel F.; Dubois, Daniel M.

    2008-10-17

    We introduce the synchronous discrete harmonic oscillator, and present an analytical, numerical and graphical study of its characteristics. The oscillator is synchronous when the time T for one revolution covering an angle of 2{pi} in phase space, is an integral multiple N of the discrete time step {delta}t. It is fully synchronous when N is even. It is pseudo-synchronous when T/{delta}t is rational. In the energy conserving hyperincursive representation, the phase space trajectories are perfectly stable at all time scales, and in both synchronous and pseudo-synchronous modes they cycle through a finite number of phase space points. Consequently, both the synchronous and the pseudo-synchronous hyperincursive modes of time-discretization provide a physically realistic and mathematically coherent, procedure for dynamic, background independent, discretization of spacetime. The procedure is applicable to any stable periodic dynamical system, and provokes an intrinsic correlation between space and time, whereby space-discretization is a direct consequence of background-independent time-discretization. Hence, synchronous discretization moves the formalism of classical mechanics towards that of special relativity. The frequency of the hyperincursive discrete harmonic oscillator is ''blue shifted'' relative to its continuum counterpart. The frequency shift has the precise value needed to make the speed of the system point in phase space independent of the discretizing time interval {delta}t. That is the speed of the system point is the same on the polygonal (in the discrete case) and the circular (in the continuum case) phase space trajectories.

  11. Compartmentalization of membrane trafficking, glucose transport, glycolysis, actin, tubulin and the proteasome in the cytoplasmic droplet/Hermes body of epididymal sperm.

    PubMed

    Au, Catherine E; Hermo, Louis; Byrne, Elliot; Smirle, Jeffrey; Fazel, Ali; Kearney, Robert E; Smith, Charles E; Vali, Hojatollah; Fernandez-Rodriguez, Julia; Simon, Paul H G; Mandato, Craig; Nilsson, Tommy; Bergeron, John J M

    2015-08-01

    Discovered in 1909 by Retzius and described mainly by morphology, the cytoplasmic droplet of sperm (renamed here the Hermes body) is conserved among all mammalian species but largely undefined at the molecular level. Tandem mass spectrometry of the isolated Hermes body from rat epididymal sperm characterized 1511 proteins, 43 of which were localized to the structure in situ by light microscopy and two by quantitative electron microscopy localization. Glucose transporter 3 (GLUT-3) glycolytic enzymes, selected membrane traffic and cytoskeletal proteins were highly abundant and concentrated in the Hermes body. By electron microscope gold antibody labelling, the Golgi trafficking protein TMED7/p27 localized to unstacked flattened cisternae of the Hermes body, as did GLUT-3, the most abundant protein. Its biogenesis was deduced through the mapping of protein expression for all 43 proteins during male germ cell differentiation in the testis. It is at the terminal step 19 of spermiogenesis that the 43 characteristic proteins accumulated in the nascent Hermes body.

  12. Compartmentalization of membrane trafficking, glucose transport, glycolysis, actin, tubulin and the proteasome in the cytoplasmic droplet/Hermes body of epididymal sperm

    PubMed Central

    Au, Catherine E.; Hermo, Louis; Byrne, Elliot; Smirle, Jeffrey; Fazel, Ali; Kearney, Robert E.; Smith, Charles E.; Vali, Hojatollah; Fernandez-Rodriguez, Julia; Simon, Paul H. G.; Mandato, Craig; Nilsson, Tommy; Bergeron, John J. M.

    2015-01-01

    Discovered in 1909 by Retzius and described mainly by morphology, the cytoplasmic droplet of sperm (renamed here the Hermes body) is conserved among all mammalian species but largely undefined at the molecular level. Tandem mass spectrometry of the isolated Hermes body from rat epididymal sperm characterized 1511 proteins, 43 of which were localized to the structure in situ by light microscopy and two by quantitative electron microscopy localization. Glucose transporter 3 (GLUT-3) glycolytic enzymes, selected membrane traffic and cytoskeletal proteins were highly abundant and concentrated in the Hermes body. By electron microscope gold antibody labelling, the Golgi trafficking protein TMED7/p27 localized to unstacked flattened cisternae of the Hermes body, as did GLUT-3, the most abundant protein. Its biogenesis was deduced through the mapping of protein expression for all 43 proteins during male germ cell differentiation in the testis. It is at the terminal step 19 of spermiogenesis that the 43 characteristic proteins accumulated in the nascent Hermes body. PMID:26311421

  13. Membrane trafficking: ER export encounters dualism.

    PubMed

    Barlowe, Charles

    2015-02-16

    Cytoplasmic coat protein complexes perform central roles in sorting protein constituents within the endomembrane system. A new study reveals that the COPII coat operates through dual recognition of signals in a sorting receptor and its bound cargo to promote efficient export from the endoplasmic reticulum. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Stability analysis of a discrete Hutchinson equation with discrete and distributed delay

    NASA Astrophysics Data System (ADS)

    Suryanto, A.; Yanti, I.; Kusumawinahyu, W. M.

    2014-02-01

    In this paper a Hutchinson equation with discrete and distributed delay is discretized by the Euler method. The dynamics of the obtained discrete system is then investigated. Specifically the stability of the positive fixed point is analyzed. It is found that for sufficiently small time-step of integration, the positive equilibrium undergoes a Neimark-Sacker bifurcation which is controlled by the discrete time delay. The results of analysis are then confirmed by some numerical simulations.

  15. Discretization vs. Rounding Error in Euler's Method

    ERIC Educational Resources Information Center

    Borges, Carlos F.

    2011-01-01

    Euler's method for solving initial value problems is an excellent vehicle for observing the relationship between discretization error and rounding error in numerical computation. Reductions in stepsize, in order to decrease discretization error, necessarily increase the number of steps and so introduce additional rounding error. The problem is…

  16. Discretization vs. Rounding Error in Euler's Method

    ERIC Educational Resources Information Center

    Borges, Carlos F.

    2011-01-01

    Euler's method for solving initial value problems is an excellent vehicle for observing the relationship between discretization error and rounding error in numerical computation. Reductions in stepsize, in order to decrease discretization error, necessarily increase the number of steps and so introduce additional rounding error. The problem is…

  17. Security: Step by Step

    ERIC Educational Resources Information Center

    Svetcov, Eric

    2005-01-01

    This article provides a list of the essential steps to keeping a school's or district's network safe and sound. It describes how to establish a security architecture and approach that will continually evolve as the threat environment changes over time. The article discusses the methodology for implementing this approach and then discusses the…

  18. Reduced discretization error in HZETRN

    SciTech Connect

    Slaba, Tony C.; Blattnig, Steve R.; Tweed, John

    2013-02-01

    The deterministic particle transport code HZETRN is an efficient analysis tool for studying the effects of space radiation on humans, electronics, and shielding materials. In a previous work, numerical methods in the code were reviewed, and new methods were developed that further improved efficiency and reduced overall discretization error. It was also shown that the remaining discretization error could be attributed to low energy light ions (A < 4) with residual ranges smaller than the physical step-size taken by the code. Accurately resolving the spectrum of low energy light particles is important in assessing risk associated with astronaut radiation exposure. In this work, modifications to the light particle transport formalism are presented that accurately resolve the spectrum of low energy light ion target fragments. The modified formalism is shown to significantly reduce overall discretization error and allows a physical approximation to be removed. For typical step-sizes and energy grids used in HZETRN, discretization errors for the revised light particle transport algorithms are shown to be less than 4% for aluminum and water shielding thicknesses as large as 100 g/cm{sup 2} exposed to both solar particle event and galactic cosmic ray environments.

  19. Reduced discretization error in HZETRN

    NASA Astrophysics Data System (ADS)

    Slaba, Tony C.; Blattnig, Steve R.; Tweed, John

    2013-02-01

    The deterministic particle transport code HZETRN is an efficient analysis tool for studying the effects of space radiation on humans, electronics, and shielding materials. In a previous work, numerical methods in the code were reviewed, and new methods were developed that further improved efficiency and reduced overall discretization error. It was also shown that the remaining discretization error could be attributed to low energy light ions (A < 4) with residual ranges smaller than the physical step-size taken by the code. Accurately resolving the spectrum of low energy light particles is important in assessing risk associated with astronaut radiation exposure. In this work, modifications to the light particle transport formalism are presented that accurately resolve the spectrum of low energy light ion target fragments. The modified formalism is shown to significantly reduce overall discretization error and allows a physical approximation to be removed. For typical step-sizes and energy grids used in HZETRN, discretization errors for the revised light particle transport algorithms are shown to be less than 4% for aluminum and water shielding thicknesses as large as 100 g/cm2 exposed to both solar particle event and galactic cosmic ray environments.

  20. Next Step for STEP

    SciTech Connect

    Wood, Claire; Bremner, Brenda

    2013-08-09

    The Siletz Tribal Energy Program (STEP), housed in the Tribe’s Planning Department, will hire a data entry coordinator to collect, enter, analyze and store all the current and future energy efficiency and renewable energy data pertaining to administrative structures the tribe owns and operates and for homes in which tribal members live. The proposed data entry coordinator will conduct an energy options analysis in collaboration with the rest of the Siletz Tribal Energy Program and Planning Department staff. An energy options analysis will result in a thorough understanding of tribal energy resources and consumption, if energy efficiency and conservation measures being implemented are having the desired effect, analysis of tribal energy loads (current and future energy consumption), and evaluation of local and commercial energy supply options. A literature search will also be conducted. In order to educate additional tribal members about renewable energy, we will send four tribal members to be trained to install and maintain solar panels, solar hot water heaters, wind turbines and/or micro-hydro.

  1. Constraint analysis for variational discrete systems

    SciTech Connect

    Dittrich, Bianca; Höhn, Philipp A.

    2013-09-15

    A canonical formalism and constraint analysis for discrete systems subject to a variational action principle are devised. The formalism is equivalent to the covariant formulation, encompasses global and local discrete time evolution moves and naturally incorporates both constant and evolving phase spaces, the latter of which is necessary for a time varying discretization. The different roles of constraints in the discrete and the conditions under which they are first or second class and/or symmetry generators are clarified. The (non-) preservation of constraints and the symplectic structure is discussed; on evolving phase spaces the number of constraints at a fixed time step depends on the initial and final time step of evolution. Moreover, the definition of observables and a reduced phase space is provided; again, on evolving phase spaces the notion of an observable as a propagating degree of freedom requires specification of an initial and final step and crucially depends on this choice, in contrast to the continuum. However, upon restriction to translation invariant systems, one regains the usual time step independence of canonical concepts. This analysis applies, e.g., to discrete mechanics, lattice field theory, quantum gravity models, and numerical analysis.

  2. Principles of Discrete Time Mechanics

    NASA Astrophysics Data System (ADS)

    Jaroszkiewicz, George

    2014-04-01

    1. Introduction; 2. The physics of discreteness; 3. The road to calculus; 4. Temporal discretization; 5. Discrete time dynamics architecture; 6. Some models; 7. Classical cellular automata; 8. The action sum; 9. Worked examples; 10. Lee's approach to discrete time mechanics; 11. Elliptic billiards; 12. The construction of system functions; 13. The classical discrete time oscillator; 14. Type 2 temporal discretization; 15. Intermission; 16. Discrete time quantum mechanics; 17. The quantized discrete time oscillator; 18. Path integrals; 19. Quantum encoding; 20. Discrete time classical field equations; 21. The discrete time Schrodinger equation; 22. The discrete time Klein-Gordon equation; 23. The discrete time Dirac equation; 24. Discrete time Maxwell's equations; 25. The discrete time Skyrme model; 26. Discrete time quantum field theory; 27. Interacting discrete time scalar fields; 28. Space, time and gravitation; 29. Causality and observation; 30. Concluding remarks; Appendix A. Coherent states; Appendix B. The time-dependent oscillator; Appendix C. Quaternions; Appendix D. Quantum registers; References; Index.

  3. Identification of parameters of discrete-continuous models

    SciTech Connect

    Cekus, Dawid Warys, Pawel

    2015-03-10

    In the paper, the parameters of a discrete-continuous model have been identified on the basis of experimental investigations and formulation of optimization problem. The discrete-continuous model represents a cantilever stepped Timoshenko beam. The mathematical model has been formulated and solved according to the Lagrange multiplier formalism. Optimization has been based on the genetic algorithm. The presented proceeding’s stages make the identification of any parameters of discrete-continuous systems possible.

  4. Discrete Gauge Theories

    NASA Astrophysics Data System (ADS)

    Alford, Mark G.; March-Russell, John

    In this review we discuss the formulation and distinguishing characteristics of discrete gauge theories, and describe several important applications of the concept. For the abelian (ℤN) discrete gauge theories, we consider the construction of the discrete charge operator F(Σ*) and the associated gauge-invariant order parameter that distinguishes different Higgs phases of a spontaneously broken U(1) gauge theory. We sketch some of the important thermodynamic consequences of the resultant discrete quantum hair on black holes. We further show that, as a consequence of unbroken discrete gauge symmetries, Grand Unified cosmic strings generically exhibit a Callan-Rubakov effect. For non-abelian discrete gauge theories we discuss in some detail the charge measurement process, and in the context of a lattice formulation we construct the non-abelian generalization of F(Σ*). This enables us to build the order parameter that distinguishes the different Higgs phases of a non-abelian discrete lattice gauge theory with matter. We also describe some of the fascinating phenomena associated with non-abelian gauge vortices. For example, we argue that a loop of Alice string, or any non-abelian string, is super-conducting by virtue of charged zero modes whose charge cannot be localized anywhere on or around the string (“Cheshire charge”). Finally, we discuss the relationship between discrete gauge theories and the existence of excitations possessing exotic spin and statistics (and more generally excitations whose interactions are purely “topological”).

  5. Discrete Element Modeling

    SciTech Connect

    Morris, J; Johnson, S

    2007-12-03

    The Distinct Element Method (also frequently referred to as the Discrete Element Method) (DEM) is a Lagrangian numerical technique where the computational domain consists of discrete solid elements which interact via compliant contacts. This can be contrasted with Finite Element Methods where the computational domain is assumed to represent a continuum (although many modern implementations of the FEM can accommodate some Distinct Element capabilities). Often the terms Discrete Element Method and Distinct Element Method are used interchangeably in the literature, although Cundall and Hart (1992) suggested that Discrete Element Methods should be a more inclusive term covering Distinct Element Methods, Displacement Discontinuity Analysis and Modal Methods. In this work, DEM specifically refers to the Distinct Element Method, where the discrete elements interact via compliant contacts, in contrast with Displacement Discontinuity Analysis where the contacts are rigid and all compliance is taken up by the adjacent intact material.

  6. On equivalence of discrete-discrete and continuum-discrete design sensitivity analysis

    NASA Technical Reports Server (NTRS)

    Choi, Kyung K.; Twu, Sung-Ling

    1989-01-01

    Developments in design sensitivity analysis (DSA) method have been made using two fundamentally different approaches as shown. In the first approach, a discretized structural finite element model is used to carry out DSA. There are three different methods in the discrete DSA approach: finite difference, semi-analytical, and analytical methods. The finite difference method is a popular one due to its simplicity, but a serious shortcoming of the method is the uncertainty in the choice of a perturbation step size of design variables. In the semi-analytical method, the derivatives of stiffness matrix is computed by finite differences, whereas in the analytical method, the derivatives are obtained analytically. For the shape design variable, computation of analytical derivative of stiffness matrix is quite costly. Because of this, the semi-analytical method is a popular choice in discrete shape DSA approach. However, recently, Barthelemy and Haftka presented that the semi-analytical method can have serious accuracy problems for shape design variables in structures modeled by beam, plate, truss, frame, and solid elements. They found that accuracy problems occur even for a simple cantilever beam. In the second approach, a continuum model of the structure is used to carry out DSA.

  7. Wheat mill stream properties for discrete element method modeling

    USDA-ARS?s Scientific Manuscript database

    A discrete phase approach based on individual wheat kernel characteristics is needed to overcome the limitations of previous statistical models and accurately predict the milling behavior of wheat. As a first step to develop a discrete element method (DEM) model for the wheat milling process, this s...

  8. Discrete dislocations in graphene

    NASA Astrophysics Data System (ADS)

    Ariza, M. P.; Ortiz, M.

    2010-05-01

    In this work, we present an application of the theory of discrete dislocations of Ariza and Ortiz (2005) to the analysis of dislocations in graphene. Specifically, we discuss the specialization of the theory to graphene and its further specialization to the force-constant model of Aizawa et al. (1990). The ability of the discrete-dislocation theory to predict dislocation core structures and energies is critically assessed for periodic arrangements of dislocation dipoles and quadrupoles. We show that, with the aid of the discrete Fourier transform, those problems are amenable to exact solution within the discrete-dislocation theory, which confers the theory a distinct advantage over conventional atomistic models. The discrete dislocations exhibit 5-7 ring core structures that are consistent with observation and result in dislocation energies that fall within the range of prediction of other models. The asymptotic behavior of dilute distributions of dislocations is characterized analytically in terms of a discrete prelogarithmic energy tensor. Explicit expressions for this discrete prelogarithmic energy tensor are provided up to quadratures.

  9. A priori discretization error metrics for distributed hydrologic modeling applications

    NASA Astrophysics Data System (ADS)

    Liu, Hongli; Tolson, Bryan A.; Craig, James R.; Shafii, Mahyar

    2016-12-01

    Watershed spatial discretization is an important step in developing a distributed hydrologic model. A key difficulty in the spatial discretization process is maintaining a balance between the aggregation-induced information loss and the increase in computational burden caused by the inclusion of additional computational units. Objective identification of an appropriate discretization scheme still remains a challenge, in part because of the lack of quantitative measures for assessing discretization quality, particularly prior to simulation. This study proposes a priori discretization error metrics to quantify the information loss of any candidate discretization scheme without having to run and calibrate a hydrologic model. These error metrics are applicable to multi-variable and multi-site discretization evaluation and provide directly interpretable information to the hydrologic modeler about discretization quality. The first metric, a subbasin error metric, quantifies the routing information loss from discretization, and the second, a hydrological response unit (HRU) error metric, improves upon existing a priori metrics by quantifying the information loss due to changes in land cover or soil type property aggregation. The metrics are straightforward to understand and easy to recode. Informed by the error metrics, a two-step discretization decision-making approach is proposed with the advantage of reducing extreme errors and meeting the user-specified discretization error targets. The metrics and decision-making approach are applied to the discretization of the Grand River watershed in Ontario, Canada. Results show that information loss increases as discretization gets coarser. Moreover, results help to explain the modeling difficulties associated with smaller upstream subbasins since the worst discretization errors and highest error variability appear in smaller upstream areas instead of larger downstream drainage areas. Hydrologic modeling experiments under

  10. The Discrete Hanging Cable

    ERIC Educational Resources Information Center

    Peters, James V.

    2004-01-01

    Using the methods of finite difference equations the discrete analogue of the parabolic and catenary cable are analysed. The fibonacci numbers and the golden ratio arise in the treatment of the catenary.

  11. The Discrete Hanging Cable

    ERIC Educational Resources Information Center

    Peters, James V.

    2004-01-01

    Using the methods of finite difference equations the discrete analogue of the parabolic and catenary cable are analysed. The fibonacci numbers and the golden ratio arise in the treatment of the catenary.

  12. Idea Exchange: On Discrete.

    ERIC Educational Resources Information Center

    Crisler, Nancy; Froelich, Gary

    1990-01-01

    Discussed are summary recommendations concerning the integration of some aspects of discrete mathematics into existing secondary mathematics courses. Outlines of course activities are grouped into the three levels of prealgebra, algebra, and geometry. Some sample problems are included. (JJK)

  13. Feedback nonlinear discrete-time systems

    NASA Astrophysics Data System (ADS)

    Yu, Miao; Wang, Jiasen; Qi, Donglian

    2014-11-01

    In this paper, we design an adaptive iterative learning control method for a class of high-order nonlinear output feedback discrete-time systems with random initial conditions and iteration-varying desired trajectories. An n-step ahead predictor approach is employed to estimate future outputs. The discrete Nussbaum gain method is incorporated into the control design to deal with unknown control directions. The proposed control algorithm ensures that the tracking error converges to zero asymptotically along the iterative learning axis except for the beginning outputs affected by random initial conditions. A numerical simulation is carried out to demonstrate the efficacy of the presented control laws.

  14. Discrete shunt controls in a Newton optimal power flow

    SciTech Connect

    Liu, W.H.E. ); Papa Iexopoulos, A.D. )

    1992-11-01

    Efficient and effective methods are needed for modeling discrete control actions in optimal power flow algorithms. Modeling discrete controls as continuous variables and rounding them off to the nearest steps is not satisfactory for controls with large step sizes such as shunt capacitors and reactors because it can significantly degrade optimality. Rigorous solution with discrete controls would involve a combinatorial research procedure which would be unacceptably slow for real-time applications. In this paper a penalty based discretization algorithm is proposed. The algorithm consistently provides a near optimal discrete solution for shunt controls without combinatorial search. It has been implemented in a production grade Newton optimal power flow program and tested on two actual power networks. Test results are reported.

  15. PHOEBE - step by step manual

    NASA Astrophysics Data System (ADS)

    Zasche, P.

    2016-03-01

    An easy step-by-step manual of PHOEBE is presented. It should serve as a starting point for the first time users of PHOEBE analyzing the eclipsing binary light curve. It is demonstrated on one particular detached system also with the downloadable data and the whole procedure is described easily till the final trustworthy fit is being reached.

  16. Annexins as Overlooked Regulators of Membrane Trafficking in Plant Cells

    PubMed Central

    Konopka-Postupolska, Dorota; Clark, Greg

    2017-01-01

    Annexins are an evolutionary conserved superfamily of proteins able to bind membrane phospholipids in a calcium-dependent manner. Their physiological roles are still being intensively examined and it seems that, despite their general structural similarity, individual proteins are specialized toward specific functions. However, due to their general ability to coordinate membranes in a calcium-sensitive fashion they are thought to participate in membrane flow. In this review, we present a summary of the current understanding of cellular transport in plant cells and consider the possible roles of annexins in different stages of vesicular transport. PMID:28422051

  17. Phosphoinositides, Major Actors in Membrane Trafficking and Lipid Signaling Pathways

    PubMed Central

    De Craene, Johan-Owen; Bertazzi, Dimitri L.; Bär, Séverine; Friant, Sylvie

    2017-01-01

    Phosphoinositides are lipids involved in the vesicular transport of proteins and lipids between the different compartments of eukaryotic cells. They act by recruiting and/or activating effector proteins and thus are involved in regulating various cellular functions, such as vesicular budding, membrane fusion and cytoskeleton dynamics. Although detected in small concentrations in membranes, their role is essential to cell function, since imbalance in their concentrations is a hallmark of many cancers. Their synthesis involves phosphorylating/dephosphorylating positions D3, D4 and/or D5 of their inositol ring by specific lipid kinases and phosphatases. This process is tightly regulated and specific to the different intracellular membranes. Most enzymes involved in phosphoinositide synthesis are conserved between yeast and human, and their loss of function leads to severe diseases (cancer, myopathy, neuropathy and ciliopathy). PMID:28294977

  18. Membrane Trafficking in the Yeast Saccharomyces cerevisiae Model

    PubMed Central

    Feyder, Serge; De Craene, Johan-Owen; Bär, Séverine; Bertazzi, Dimitri L.; Friant, Sylvie

    2015-01-01

    The yeast Saccharomyces cerevisiae is one of the best characterized eukaryotic models. The secretory pathway was the first trafficking pathway clearly understood mainly thanks to the work done in the laboratory of Randy Schekman in the 1980s. They have isolated yeast sec mutants unable to secrete an extracellular enzyme and these SEC genes were identified as encoding key effectors of the secretory machinery. For this work, the 2013 Nobel Prize in Physiology and Medicine has been awarded to Randy Schekman; the prize is shared with James Rothman and Thomas Südhof. Here, we present the different trafficking pathways of yeast S. cerevisiae. At the Golgi apparatus newly synthesized proteins are sorted between those transported to the plasma membrane (PM), or the external medium, via the exocytosis or secretory pathway (SEC), and those targeted to the vacuole either through endosomes (vacuolar protein sorting or VPS pathway) or directly (alkaline phosphatase or ALP pathway). Plasma membrane proteins can be internalized by endocytosis (END) and transported to endosomes where they are sorted between those targeted for vacuolar degradation and those redirected to the Golgi (recycling or RCY pathway). Studies in yeast S. cerevisiae allowed the identification of most of the known effectors, protein complexes, and trafficking pathways in eukaryotic cells, and most of them are conserved among eukaryotes. PMID:25584613

  19. Membrane trafficking: decoding vesicle identity with contrasting chemistries.

    PubMed

    Frost, Adam

    2011-10-11

    Proteins involved in membrane traffic must distinguish between different classes of vesicles. New work now shows that α-synuclein and ALPS motifs represent two extreme types of amphipathic helix that are tuned to detect both the curvature of transport vesicles as well as their bulk lipid content.

  20. Membrane trafficking in the yeast Saccharomyces cerevisiae model.

    PubMed

    Feyder, Serge; De Craene, Johan-Owen; Bär, Séverine; Bertazzi, Dimitri L; Friant, Sylvie

    2015-01-09

    The yeast Saccharomyces cerevisiae is one of the best characterized eukaryotic models. The secretory pathway was the first trafficking pathway clearly understood mainly thanks to the work done in the laboratory of Randy Schekman in the 1980s. They have isolated yeast sec mutants unable to secrete an extracellular enzyme and these SEC genes were identified as encoding key effectors of the secretory machinery. For this work, the 2013 Nobel Prize in Physiology and Medicine has been awarded to Randy Schekman; the prize is shared with James Rothman and Thomas Südhof. Here, we present the different trafficking pathways of yeast S. cerevisiae. At the Golgi apparatus newly synthesized proteins are sorted between those transported to the plasma membrane (PM), or the external medium, via the exocytosis or secretory pathway (SEC), and those targeted to the vacuole either through endosomes (vacuolar protein sorting or VPS pathway) or directly (alkaline phosphatase or ALP pathway). Plasma membrane proteins can be internalized by endocytosis (END) and transported to endosomes where they are sorted between those targeted for vacuolar degradation and those redirected to the Golgi (recycling or RCY pathway). Studies in yeast S. cerevisiae allowed the identification of most of the known effectors, protein complexes, and trafficking pathways in eukaryotic cells, and most of them are conserved among eukaryotes.

  1. Numerical discretization for nonlinear diffusion filter

    NASA Astrophysics Data System (ADS)

    Mustaffa, I.; Mizuar, I.; Aminuddin, M. M. M.; Dasril, Y.

    2015-05-01

    Nonlinear diffusion filters are famously used in machine vision for image denoising and restoration. This paper presents a study on the effects of different numerical discretization of nonlinear diffusion filter. Several numerical discretization schemes are presented; namely semi-implicit, AOS, and fully implicit schemes. The results of these schemes are compared by visual results, objective measurement e.g. PSNR and MSE. The results are also compared to a Daubechies wavelet denoising method. It is acknowledged that the two preceding scheme have already been discussed in literature, however comparison to the latter scheme has not been made. The semi-implicit scheme uses an additive operator splitting (AOS) developed to overcome the shortcoming of the explicit scheme i.e., stability for very small time steps. Although AOS has proven to be efficient, from the nonlinear diffusion filter results with different discretization schemes, examples shows that implicit schemes are worth pursuing.

  2. Nano Step

    NASA Image and Video Library

    2012-09-25

    ISS033-E-007358 (25 Sept. 2012) --- Japan Aerospace Exploration Agency astronaut Aki Hoshide, Expedition 33 flight engineer, services the Nano Step payload in the Kibo laboratory of the International Space Station.

  3. Depression: discrete or continuous?

    PubMed

    Bowins, Brad

    2015-01-01

    Elucidating the true structure of depression is necessary if we are to advance our understanding and treatment options. Central to the issue of structure is whether depression represents discrete types or occurs on a continuum. Nature almost universally operates on the basis of continuums, whereas human perception favors discrete categories. This reality might be formalized into a 'continuum principle': natural phenomena tend to occur on a continuum, and any instance of hypothesized discreteness requires unassailable proof. Research evidence for discrete types falls far short of this standard, with most evidence supporting a continuum. However, quantitative variation can yield qualitative differences as an emergent property, fostering the appearance of discreteness. Depression as a continuum is best characterized by duration and severity dimensions, with the latter understood in terms of depressive inhibition. In the absence of some degree of cognitive, emotional, social, and physical inhibition, depression should not be diagnosed. Combining the dimensions of duration and severity provides an optimal way to characterize the quantitative and related qualitative aspects of depression and to describe the overall degree of dysfunction. The presence of other symptom types occurs when anxiety, hypomanic/manic, psychotic, and personality continuums interface with the depression continuum.

  4. Discrete Newtonian cosmology: perturbations

    NASA Astrophysics Data System (ADS)

    Ellis, George F. R.; Gibbons, Gary W.

    2015-03-01

    In a previous paper (Gibbons and Ellis 2014 Discrete Newtonian cosmology Class. Quantum Grav. 31 025003), we showed how a finite system of discrete particles interacting with each other via Newtonian gravitational attraction would lead to precisely the same dynamical equations for homothetic motion as in the case of the pressure-free Friedmann-Lemaître-Robertson-Walker cosmological models of general relativity theory, provided the distribution of particles obeys the central configuration equation. In this paper we show that one can obtain perturbed such Newtonian solutions that give the same linearized structure growth equations as in the general relativity case. We also obtain the Dmitriev-Zel’dovich equations for subsystems in this discrete gravitational model, and show how it leads to the conclusion that voids have an apparent negative mass.

  5. Discrete Driver Assistance

    NASA Astrophysics Data System (ADS)

    Klette, Reinhard; Jiang, Ruyi; Morales, Sandino; Vaudrey, Tobi

    Applying computer technology, such as computer vision in driver assistance, implies that processes and data are modeled as being discretized rather than being continuous. The area of stereo vision provides various examples how concepts known in discrete mathematics (e.g., pixel adjacency graphs, belief propagation, dynamic programming, max-flow/min-cut, or digital straight lines) are applied when aiming for efficient and accurate pixel correspondence solutions. The paper reviews such developments for a reader in discrete mathematics who is interested in applied research (in particular, in vision-based driver assistance). As a second subject, the paper also discusses lane detection and tracking, which is a particular task in driver assistance; recently the Euclidean distance transform proved to be a very appropriate tool for obtaining a fairly robust solution.

  6. IL-6 blocks a discrete early step in lymphopoiesis.

    PubMed

    Maeda, Kazuhiko; Baba, Yoshihiro; Nagai, Yoshinori; Miyazaki, Kozo; Malykhin, Alexander; Nakamura, Koji; Kincade, Paul W; Sakaguchi, Nobuo; Coggeshall, K Mark

    2005-08-01

    Animals lacking Src homology 2 domain-containing inositol 5-phosphatase (SHIP) display a reduction in lymphopoiesis and a corresponding enhancement of myelopoiesis. These effects are mediated at least in part by elevated levels of interleukin 6 (IL-6). Here, we show the lymphopoiesis block in SHIP-/- mice is due to suppression of the lymphoid lineage choice by uncommitted progenitors. The suppression can be reproduced in vitro with recombinant IL-6, and IL-6 acts directly on hematopoietic progenitors. The block is partially overcome in SHIP-/- IL-6-/- double-deficient animals. IL-6 does not suppress but actually enhances proliferation of lymphoid-committed progenitors, indicating the IL-6 target cells are hematopoietic stem cells or multipotent progenitors. The findings suggest a mechanism for the lymphopenia that accompanies proinflammatory diseases.

  7. IL-6 blocks a discrete early step in lymphopoiesis

    PubMed Central

    Maeda, Kazuhiko; Baba, Yoshihiro; Nagai, Yoshinori; Miyazaki, Kozo; Malykhin, Alexander; Nakamura, Koji; Kincade, Paul W.; Sakaguchi, Nobuo; Coggeshall, K. Mark

    2005-01-01

    Animals lacking Src homology 2 domain-containing inositol 5-phosphatase (SHIP) display a reduction in lymphopoiesis and a corresponding enhancement of myelopoiesis. These effects are mediated at least in part by elevated levels of interleukin 6 (IL-6). Here, we show the lymphopoiesis block in SHIP–/– mice is due to suppression of the lymphoid lineage choice by uncommitted progenitors. The suppression can be reproduced in vitro with recombinant IL-6, and IL-6 acts directly on hematopoietic progenitors. The block is partially overcome in SHIP–/– IL-6–/– double-deficient animals. IL-6 does not suppress but actually enhances proliferation of lymphoid-committed progenitors, indicating the IL-6 target cells are hematopoietic stem cells or multipotent progenitors. The findings suggest a mechanism for the lymphopenia that accompanies proinflammatory diseases. PMID:15831701

  8. Discrete breathers in crystals

    NASA Astrophysics Data System (ADS)

    Dmitriev, S. V.; Korznikova, E. A.; Baimova, Yu A.; Velarde, M. G.

    2016-05-01

    It is well known that periodic discrete defect-containing systems, in addition to traveling waves, support vibrational defect-localized modes. It turned out that if a periodic discrete system is nonlinear, it can support spatially localized vibrational modes as exact solutions even in the absence of defects. Since the nodes of the system are all on equal footing, it is only through the special choice of initial conditions that a group of nodes can be found on which such a mode, called a discrete breather (DB), will be excited. The DB frequency must be outside the frequency range of the small-amplitude traveling waves. Not resonating with and expending no energy on the excitation of traveling waves, a DB can theoretically conserve its vibrational energy forever provided no thermal vibrations or other perturbations are present. Crystals are nonlinear discrete systems, and the discovery in them of DBs was only a matter of time. It is well known that periodic discrete defect-containing systems support both traveling waves and vibrational defect-localized modes. It turns out that if a periodic discrete system is nonlinear, it can support spatially localized vibrational modes as exact solutions even in the absence of defects. Because the nodes of the system are all on equal footing, only a special choice of the initial conditions allows selecting a group of nodes on which such a mode, called a discrete breather (DB), can be excited. The DB frequency must be outside the frequency range of small-amplitude traveling waves. Not resonating with and expending no energy on the excitation of traveling waves, a DB can theoretically preserve its vibrational energy forever if no thermal vibrations or other perturbations are present. Crystals are nonlinear discrete systems, and the discovery of DBs in them was only a matter of time. Experimental studies of DBs encounter major technical difficulties, leaving atomistic computer simulations as the primary investigation tool. Despite

  9. Joint discrete universality of Hurwitz zeta functions

    SciTech Connect

    Laurinčikas, A

    2014-11-30

    We obtain a joint discrete universality theorem for Hurwitz zeta functions. Here the parameters of zeta functions and the step of shifts of these functions approximating a given family of analytic functions are connected by some condition of linear independence. Nesterenko's theorem gives an example satisfying this condition. The universality theorem is applied to estimate the number of zeros of a linear combination of Hurwitz zeta functions. Bibliography: 20 titles.

  10. Discrete surface solitons.

    PubMed

    Makris, Konstantinos G; Suntsov, Sergiy; Christodoulides, Demetrios N; Stegeman, George I; Hache, Alain

    2005-09-15

    It is theoretically shown that discrete nonlinear surface waves are possible in waveguide lattices. These self-trapped states are located at the edge of the array and can exist only above a certain power threshold. The excitation characteristics and stability properties of these surface waves are systematically investigated.

  11. Quantum cosmology based on discrete Feynman paths

    SciTech Connect

    Chew, Geoffrey F.

    2002-10-10

    Although the rules for interpreting local quantum theory imply discretization of process, Lorentz covariance is usually regarded as precluding time quantization. Nevertheless a time-discretized quantum representation of redshifting spatially-homogeneous universe may be based on discrete-step Feynman paths carrying causal Lorentz-invariant action--paths that not only propagate the wave function but provide a phenomenologically-promising elementary-particle Hilbert-space basis. In a model under development, local path steps are at Planck scale while, at a much larger ''wave-function scale'', global steps separate successive wave-functions. Wave-function spacetime is but a tiny fraction of path spacetime. Electromagnetic and gravitational actions are ''at a distance'' in Wheeler-Feynman sense while strong (color) and weak (isospin) actions, as well as action of particle motion, are ''local'' in a sense paralleling the action of local field theory. ''Nonmaterial'' path segments and ''trivial events'' collaborate to define energy and gravity. Photons coupled to conserved electric charge enjoy privileged model status among elementary fermions and vector bosons. Although real path parameters provide no immediate meaning for ''measurement'', the phase of the complex wave function allows significance for ''information'' accumulated through ''gentle'' electromagnetic events involving charged matter and ''soft'' photons. Through its soft-photon content the wave function is an ''information reservoir''.

  12. Semiconductor step-stress testing

    NASA Technical Reports Server (NTRS)

    1980-01-01

    Report describes extensive program to test behavior of discrete diodes and transistors subjected to power and temperature overstress. Commercially available bipolar and field effect transistors and diodes were stressed between 0.5 and 1.75 times maximum rated power. Two groups were temperature stressed: 160 hour steps starting at 75 C to maximum of 300 C. Cumulative failures and changes in device parameters were monitored and reasons for failures presented.

  13. Discrete Variational Optimal Control

    NASA Astrophysics Data System (ADS)

    Jiménez, Fernando; Kobilarov, Marin; Martín de Diego, David

    2013-06-01

    This paper develops numerical methods for optimal control of mechanical systems in the Lagrangian setting. It extends the theory of discrete mechanics to enable the solutions of optimal control problems through the discretization of variational principles. The key point is to solve the optimal control problem as a variational integrator of a specially constructed higher dimensional system. The developed framework applies to systems on tangent bundles, Lie groups, and underactuated and nonholonomic systems with symmetries, and can approximate either smooth or discontinuous control inputs. The resulting methods inherit the preservation properties of variational integrators and result in numerically robust and easily implementable algorithms. Several theoretical examples and a practical one, the control of an underwater vehicle, illustrate the application of the proposed approach.

  14. Discrete minimal flavor violation

    SciTech Connect

    Zwicky, Roman; Fischbacher, Thomas

    2009-10-01

    We investigate the consequences of replacing the global flavor symmetry of minimal flavor violation (MFV) SU(3){sub Q}xSU(3){sub U}xSU(3){sub D}x{center_dot}{center_dot}{center_dot} by a discrete D{sub Q}xD{sub U}xD{sub D}x{center_dot}{center_dot}{center_dot} symmetry. Goldstone bosons resulting from the breaking of the flavor symmetry generically lead to bounds on new flavor structure many orders of magnitude above the TeV scale. The absence of Goldstone bosons for discrete symmetries constitute the primary motivation of our work. Less symmetry implies further invariants and renders the mass-flavor basis transformation observable in principle and calls for a hierarchy in the Yukawa matrix expansion. We show, through the dimension of the representations, that the (discrete) symmetry in principle does allow for additional {delta}F=2 operators. If though the {delta}F=2 transitions are generated by two subsequent {delta}F=1 processes, as, for example, in the standard model, then the four crystal-like groups {sigma}(168){approx_equal}PSL(2,F{sub 7}), {sigma}(72{phi}), {sigma}(216{phi}) and especially {sigma}(360{phi}) do provide enough protection for a TeV-scale discrete MFV scenario. Models where this is not the case have to be investigated case by case. Interestingly {sigma}(216{phi}) has a (nonfaithful) representation corresponding to an A{sub 4} symmetry. Moreover we argue that the, apparently often omitted, (D) groups are subgroups of an appropriate {delta}(6g{sup 2}). We would like to stress that we do not provide an actual model that realizes the MFV scenario nor any other theory of flavor.

  15. The Discrete Wavelet Transform

    DTIC Science & Technology

    1991-06-01

    Split- Band Coding," Proc. ICASSP, May 1977, pp 191-195. 12. Vetterli, M. "A Theory of Multirate Filter Banks ," IEEE Trans. ASSP, 35, March 1987, pp 356...both special cases of a single filter bank structure, the discrete wavelet transform, the behavior of which is governed by one’s choice of filters . In...B-1 ,.iii FIGURES 1.1 A wavelet filter bank structure ..................................... 2 2.1 Diagram illustrating the dialation and

  16. Steerable Discrete Fourier Transform

    NASA Astrophysics Data System (ADS)

    Fracastoro, Giulia; Magli, Enrico

    2017-03-01

    Directional transforms have recently raised a lot of interest thanks to their numerous applications in signal compression and analysis. In this letter, we introduce a generalization of the discrete Fourier transform, called steerable DFT (SDFT). Since the DFT is used in numerous fields, it may be of interest in a wide range of applications. Moreover, we also show that the SDFT is highly related to other well-known transforms, such as the Fourier sine and cosine transforms and the Hilbert transforms.

  17. A paradigm for discrete physics

    SciTech Connect

    Noyes, H.P.; McGoveran, D.; Etter, T.; Manthey, M.J.; Gefwert, C.

    1987-01-01

    An example is outlined for constructing a discrete physics using as a starting point the insight from quantum physics that events are discrete, indivisible and non-local. Initial postulates are finiteness, discreteness, finite computability, absolute nonuniqueness (i.e., homogeneity in the absence of specific cause) and additivity.

  18. Stepped nozzle

    DOEpatents

    Sutton, G.P.

    1998-07-14

    An insert is described which allows a supersonic nozzle of a rocket propulsion system to operate at two or more different nozzle area ratios. This provides an improved vehicle flight performance or increased payload. The insert has significant advantages over existing devices for increasing nozzle area ratios. The insert is temporarily fastened by a simple retaining mechanism to the aft end of the diverging segment of the nozzle and provides for a multi-step variation of nozzle area ratio. When mounted in place, the insert provides the nozzle with a low nozzle area ratio. During flight, the retaining mechanism is released and the insert ejected thereby providing a high nozzle area ratio in the diverging nozzle segment. 5 figs.

  19. Stepped nozzle

    DOEpatents

    Sutton, George P.

    1998-01-01

    An insert which allows a supersonic nozzle of a rocket propulsion system to operate at two or more different nozzle area ratios. This provides an improved vehicle flight performance or increased payload. The insert has significant advantages over existing devices for increasing nozzle area ratios. The insert is temporarily fastened by a simple retaining mechanism to the aft end of the diverging segment of the nozzle and provides for a multi-step variation of nozzle area ratio. When mounted in place, the insert provides the nozzle with a low nozzle area ratio. During flight, the retaining mechanism is released and the insert ejected thereby providing a high nozzle area ratio in the diverging nozzle segment.

  20. Discrete epidemic models.

    PubMed

    Brauer, Fred; Feng, Zhilan; Castillo-Chavez, Carlos

    2010-01-01

    The mathematical theory of single outbreak epidemic models really began with the work of Kermack and Mackendrick about decades ago. This gave a simple answer to the long-standing question of why epidemics woould appear suddenly and then disappear just as suddenly without having infected an entire population. Therefore it seemed natural to expect that theoreticians would immediately proceed to expand this mathematical framework both because the need to handle recurrent single infectious disease outbreaks has always been a priority for public health officials and because theoreticians often try to push the limits of exiting theories. However, the expansion of the theory via the inclusion of refined epidemiological classifications or through the incorporation of categories that are essential for the evaluation of intervention strategies, in the context of ongoing epidemic outbreaks, did not materialize. It was the global threat posed by SARS in that caused theoreticians to expand the Kermack-McKendrick single-outbreak framework. Most recently, efforts to connect theoretical work to data have exploded as attempts to deal with the threat of emergent and re-emergent diseases including the most recent H1N1 influenza pandemic, have marched to the forefront of our global priorities. Since data are collected and/or reported over discrete units of time, developing single outbreak models that fit collected data naturally is relevant. In this note, we introduce a discrete-epidemic framework and highlight, through our analyses, the similarities between single-outbreak comparable classical continuous-time epidemic models and the discrete-time models introduced in this note. The emphasis is on comparisons driven by expressions for the final epidemic size.

  1. Discrete BPS skyrmions

    NASA Astrophysics Data System (ADS)

    Agaoglou, M.; Charalampidis, E. G.; Ioannidou, T. A.; Kevrekidis, P. G.

    2017-09-01

    A discrete analogue of the extended Bogomolny-Prasad-Sommerfeld (BPS) Skyrme model that admits time-dependent solutions is presented. Using the spacing h of adjacent lattice nodes as a parameter, we identify the spatial profile of the solution and the continuation of the relevant branch of solutions over the lattice spacing for different values of the potential (free) parameter α . In particular, we explore the dynamics and stability of the obtained solutions, finding that, while they generally seem to be prone to instabilities, for suitable values of the lattice spacing and for sufficiently large values of α , they may be long-lived in direct numerical simulations.

  2. Discrete Dynamics Lab

    NASA Astrophysics Data System (ADS)

    Wuensche, Andrew

    DDLab is interactive graphics software for creating, visualizing, and analyzing many aspects of Cellular Automata, Random Boolean Networks, and Discrete Dynamical Networks in general and studying their behavior, both from the time-series perspective — space-time patterns, and from the state-space perspective — attractor basins. DDLab is relevant to research, applications, and education in the fields of complexity, self-organization, emergent phenomena, chaos, collision-based computing, neural networks, content addressable memory, genetic regulatory networks, dynamical encryption, generative art and music, and the study of the abstract mathematical/physical/dynamical phenomena in their own right.

  3. GENERAL: Symplectic-energy-first integrators of discrete mechanico-electrical dynamical systems

    NASA Astrophysics Data System (ADS)

    Fu, Jing-Li; Chen, Ben-Yong; Tang, Yi-Fa; Fu, Hao

    2008-11-01

    A discrete total variation calculus with variable time steps is presented for mechanico-electrical systems where there exist non-potential and dissipative forces. By using this discrete variation calculus, the symplectic-energy-first integrators for mechanico-electrical systems are derived. To do this, the time step adaptation is employed. The discrete variational principle and the Euler-Lagrange equation are derived for the systems. By using this discrete algorithm it is shown that mechanico-electrical systems are not symplectic and their energies are not conserved unless they are Lagrange mechanico-electrical systems. A practical example is presented to illustrate these results.

  4. A priori discretization quality metrics for distributed hydrologic modeling applications

    NASA Astrophysics Data System (ADS)

    Liu, Hongli; Tolson, Bryan; Craig, James; Shafii, Mahyar; Basu, Nandita

    2016-04-01

    In distributed hydrologic modelling, a watershed is treated as a set of small homogeneous units that address the spatial heterogeneity of the watershed being simulated. The ability of models to reproduce observed spatial patterns firstly depends on the spatial discretization, which is the process of defining homogeneous units in the form of grid cells, subwatersheds, or hydrologic response units etc. It is common for hydrologic modelling studies to simply adopt a nominal or default discretization strategy without formally assessing alternative discretization levels. This approach lacks formal justifications and is thus problematic. More formalized discretization strategies are either a priori or a posteriori with respect to building and running a hydrologic simulation model. A posteriori approaches tend to be ad-hoc and compare model calibration and/or validation performance under various watershed discretizations. The construction and calibration of multiple versions of a distributed model can become a seriously limiting computational burden. Current a priori approaches are more formalized and compare overall heterogeneity statistics of dominant variables between candidate discretization schemes and input data or reference zones. While a priori approaches are efficient and do not require running a hydrologic model, they do not fully investigate the internal spatial pattern changes of variables of interest. Furthermore, the existing a priori approaches focus on landscape and soil data and do not assess impacts of discretization on stream channel definition even though its significance has been noted by numerous studies. The primary goals of this study are to (1) introduce new a priori discretization quality metrics considering the spatial pattern changes of model input data; (2) introduce a two-step discretization decision-making approach to compress extreme errors and meet user-specified discretization expectations through non-uniform discretization threshold

  5. Integrable discrete PT symmetric model.

    PubMed

    Ablowitz, Mark J; Musslimani, Ziad H

    2014-09-01

    An exactly solvable discrete PT invariant nonlinear Schrödinger-like model is introduced. It is an integrable Hamiltonian system that exhibits a nontrivial nonlinear PT symmetry. A discrete one-soliton solution is constructed using a left-right Riemann-Hilbert formulation. It is shown that this pure soliton exhibits unique features such as power oscillations and singularity formation. The proposed model can be viewed as a discretization of a recently obtained integrable nonlocal nonlinear Schrödinger equation.

  6. Discrete spectrum of inflationary fluctuations

    SciTech Connect

    Hogan, Craig J.

    2004-10-15

    It is conjectured that inflation, taking account of quantum gravity, leads to a discrete spectrum of cosmological perturbations, instead of the continuous Gaussian spectrum predicted by standard field theory in an unquantized background. Heuristic models of discrete spectra are discussed, based on an inflaton mode with self-gravity, a lattice of amplitude states, an entangled ensemble of modes, and the holographic or covariant entropy bound. Estimates are given for the discreteness observable in cosmic background anisotropy, galaxy clustering, and gravitational wave backgrounds.

  7. Nonintegrable Schrodinger discrete breathers.

    PubMed

    Gómez-Gardeñes, J; Floría, L M; Peyrard, M; Bishop, A R

    2004-12-01

    In an extensive numerical investigation of nonintegrable translational motion of discrete breathers in nonlinear Schrödinger lattices, we have used a regularized Newton algorithm to continue these solutions from the limit of the integrable Ablowitz-Ladik lattice. These solutions are shown to be a superposition of a localized moving core and an excited extended state (background) to which the localized moving pulse is spatially asymptotic. The background is a linear combination of small amplitude nonlinear resonant plane waves and it plays an essential role in the energy balance governing the translational motion of the localized core. Perturbative collective variable theory predictions are critically analyzed in the light of the numerical results.

  8. Discrete bisoliton fiber laser

    PubMed Central

    Liu, X. M.; Han, X. X.; Yao, X. K.

    2016-01-01

    Dissipative solitons, which result from the intricate balance between dispersion and nonlinearity as well as gain and loss, are of the fundamental scientific interest and numerous important applications. Here, we report a fiber laser that generates bisoliton – two consecutive dissipative solitons that preserve a fixed separation between them. Deviations from this separation result in its restoration. It is also found that these bisolitons have multiple discrete equilibrium distances with the quantized separations, as is confirmed by the theoretical analysis and the experimental observations. The main feature of our laser is the anomalous dispersion that is increased by an order of magnitude in comparison to previous studies. Then the spectral filtering effect plays a significant role in pulse-shaping. The proposed laser has the potential applications in optical communications and high-resolution optics for coding and transmission of information in higher-level modulation formats. PMID:27767075

  9. Discrete anti-gravity

    NASA Astrophysics Data System (ADS)

    Noyes, H. Pierre; Starson, Scott

    1991-03-01

    Discrete physics, because it replaces time evolution generated by the energy operator with a global bit-string generator (program universe) and replaces fields with the relativistic Wheeler-Feynman action at a distance, allows the consistent formulation of the concept of signed gravitational charge for massive particles. The resulting prediction made by this version of the theory is that free anti-particles near the surface of the earth will fall up with the same acceleration that the corresponding particles fall down. So far as we can see, no current experimental information is in conflict with this prediction of our theory. The experiment crusis will be one of the anti-proton or anti-hydrogen experiments at CERN. Our prediction should be much easier to test than the small effects which those experiments are currently designed to detect or bound.

  10. Discrete bisoliton fiber laser

    NASA Astrophysics Data System (ADS)

    Liu, X. M.; Han, X. X.; Yao, X. K.

    2016-10-01

    Dissipative solitons, which result from the intricate balance between dispersion and nonlinearity as well as gain and loss, are of the fundamental scientific interest and numerous important applications. Here, we report a fiber laser that generates bisoliton – two consecutive dissipative solitons that preserve a fixed separation between them. Deviations from this separation result in its restoration. It is also found that these bisolitons have multiple discrete equilibrium distances with the quantized separations, as is confirmed by the theoretical analysis and the experimental observations. The main feature of our laser is the anomalous dispersion that is increased by an order of magnitude in comparison to previous studies. Then the spectral filtering effect plays a significant role in pulse-shaping. The proposed laser has the potential applications in optical communications and high-resolution optics for coding and transmission of information in higher-level modulation formats.

  11. Steerable Discrete Cosine Transform

    NASA Astrophysics Data System (ADS)

    Fracastoro, Giulia; Fosson, Sophie M.; Magli, Enrico

    2017-01-01

    In image compression, classical block-based separable transforms tend to be inefficient when image blocks contain arbitrarily shaped discontinuities. For this reason, transforms incorporating directional information are an appealing alternative. In this paper, we propose a new approach to this problem, namely a discrete cosine transform (DCT) that can be steered in any chosen direction. Such transform, called steerable DCT (SDCT), allows to rotate in a flexible way pairs of basis vectors, and enables precise matching of directionality in each image block, achieving improved coding efficiency. The optimal rotation angles for SDCT can be represented as solution of a suitable rate-distortion (RD) problem. We propose iterative methods to search such solution, and we develop a fully fledged image encoder to practically compare our techniques with other competing transforms. Analytical and numerical results prove that SDCT outperforms both DCT and state-of-the-art directional transforms.

  12. Steerable Discrete Cosine Transform.

    PubMed

    Fracastoro, Giulia; Fosson, Sophie M; Magli, Enrico

    2017-01-01

    In image compression, classical block-based separable transforms tend to be inefficient when image blocks contain arbitrarily shaped discontinuities. For this reason, transforms incorporating directional information are an appealing alternative. In this paper, we propose a new approach to this problem, namely, a discrete cosine transform (DCT) that can be steered in any chosen direction. Such transform, called steerable DCT (SDCT), allows to rotate in a flexible way pairs of basis vectors, and enables precise matching of directionality in each image block, achieving improved coding efficiency. The optimal rotation angles for SDCT can be represented as solution of a suitable rate-distortion (RD) problem. We propose iterative methods to search such solution, and we develop a fully fledged image encoder to practically compare our techniques with other competing transforms. Analytical and numerical results prove that SDCT outperforms both DCT and state-of-the-art directional transforms.

  13. Discrete anti-gravity

    SciTech Connect

    Noyes, H.P. ); Starson, S. )

    1991-03-01

    Discrete physics, because it replaces time evolution generated by the energy operator with a global bit-string generator (program universe) and replaces fields'' with the relativistic Wheeler-Feynman action at a distance,'' allows the consistent formulation of the concept of signed gravitational charge for massive particles. The resulting prediction made by this version of the theory is that free anti-particles near the surface of the earth will fall'' up with the same acceleration that the corresponding particles fall down. So far as we can see, no current experimental information is in conflict with this prediction of our theory. The experiment crusis will be one of the anti-proton or anti-hydrogen experiments at CERN. Our prediction should be much easier to test than the small effects which those experiments are currently designed to detect or bound. 23 refs.

  14. Discrete Pearson distributions

    SciTech Connect

    Bowman, K.O.; Shenton, L.R.; Kastenbaum, M.A.

    1991-11-01

    These distributions are generated by a first order recursive scheme which equates the ratio of successive probabilities to the ratio of two corresponding quadratics. The use of a linearized form of this model will produce equations in the unknowns matched by an appropriate set of moments (assumed to exist). Given the moments we may find valid solutions. These are two cases; (1) distributions defined on the non-negative integers (finite or infinite) and (2) distributions defined on negative integers as well. For (1), given the first four moments, it is possible to set this up as equations of finite or infinite degree in the probability of a zero occurrence, the sth component being a product of s ratios of linear forms in this probability in general. For (2) the equation for the zero probability is purely linear but may involve slowly converging series; here a particular case is the discrete normal. Regions of validity are being studied. 11 refs.

  15. Discrete Reliability Projection

    DTIC Science & Technology

    2014-12-01

    Defense, Handbook MIL - HDBK -189C, 2011 Hall, J. B., Methodology for Evaluating Reliability Growth Programs of Discrete Systems, Ph.D. thesis, University...pk,i ] · [ 1− (1− θ̆k) · ( N k · T )]k−m , (2.13) 5 2 Hall’s Model where m is the number of observed failure modes and d∗i estimates di (either based...Mode Failures FEF Ni d ∗ i 1 1 0.95 2 1 0.70 3 1 0.90 4 1 0.90 5 4 0.95 6 2 0.70 7 1 0.80 Using equations 2.1 and 2.2 we can calculate the failure

  16. Immigration and Prosecutorial Discretion.

    PubMed

    Apollonio, Dorie; Lochner, Todd; Heddens, Myriah

    Immigration has become an increasingly salient national issue in the US, and the Department of Justice recently increased federal efforts to prosecute immigration offenses. This shift, however, relies on the cooperation of US attorneys and their assistants. Traditionally federal prosecutors have enjoyed enormous discretion and have been responsive to local concerns. To consider how the centralized goal of immigration enforcement may have influenced federal prosecutors in regional offices, we review their prosecution of immigration offenses in California using over a decade's worth of data. Our findings suggest that although centralizing forces influence immigration prosecutions, individual US attorneys' offices retain distinct characteristics. Local factors influence federal prosecutors' behavior in different ways depending on the office. Contrary to expectations, unemployment rates did not affect prosecutors' willingness to pursue immigration offenses, nor did local popular opinion about illegal immigration.

  17. Discrete Minimal Surface Algebras

    NASA Astrophysics Data System (ADS)

    Arnlind, Joakim; Hoppe, Jens

    2010-05-01

    We consider discrete minimal surface algebras (DMSA) as generalized noncommutative analogues of minimal surfaces in higher dimensional spheres. These algebras appear naturally in membrane theory, where sequences of their representations are used as a regularization. After showing that the defining relations of the algebra are consistent, and that one can compute a basis of the enveloping algebra, we give several explicit examples of DMSAs in terms of subsets of sln (any semi-simple Lie algebra providing a trivial example by itself). A special class of DMSAs are Yang-Mills algebras. The representation graph is introduced to study representations of DMSAs of dimension d ≤ 4, and properties of representations are related to properties of graphs. The representation graph of a tensor product is (generically) the Cartesian product of the corresponding graphs. We provide explicit examples of irreducible representations and, for coinciding eigenvalues, classify all the unitary representations of the corresponding algebras.

  18. Immigration and Prosecutorial Discretion

    PubMed Central

    Apollonio, Dorie; Lochner, Todd; Heddens, Myriah

    2015-01-01

    Immigration has become an increasingly salient national issue in the US, and the Department of Justice recently increased federal efforts to prosecute immigration offenses. This shift, however, relies on the cooperation of US attorneys and their assistants. Traditionally federal prosecutors have enjoyed enormous discretion and have been responsive to local concerns. To consider how the centralized goal of immigration enforcement may have influenced federal prosecutors in regional offices, we review their prosecution of immigration offenses in California using over a decade's worth of data. Our findings suggest that although centralizing forces influence immigration prosecutions, individual US attorneys' offices retain distinct characteristics. Local factors influence federal prosecutors' behavior in different ways depending on the office. Contrary to expectations, unemployment rates did not affect prosecutors' willingness to pursue immigration offenses, nor did local popular opinion about illegal immigration. PMID:26146530

  19. Thermodynamics of discrete quantum processes

    NASA Astrophysics Data System (ADS)

    Anders, Janet; Giovannetti, Vittorio

    2013-03-01

    We define thermodynamic configurations and identify two primitives of discrete quantum processes between configurations for which heat and work can be defined in a natural way. This allows us to uncover a general second law for any discrete trajectory that consists of a sequence of these primitives, linking both equilibrium and non-equilibrium configurations. Moreover, in the limit of a discrete trajectory that passes through an infinite number of configurations, i.e. in the reversible limit, we recover the saturation of the second law. Finally, we show that for a discrete Carnot cycle operating between four configurations one recovers Carnot's thermal efficiency.

  20. Properties of wavelet discretization of Black-Scholes equation

    NASA Astrophysics Data System (ADS)

    Finěk, Václav

    2017-07-01

    Using wavelet methods, the continuous problem is transformed into a well-conditioned discrete problem. And once a non-symmetric problem is given, squaring yields a symmetric positive definite formulation. However squaring usually makes the condition number of discrete problems substantially worse. This note is concerned with a wavelet based numerical solution of the Black-Scholes equation for pricing European options. We show here that in wavelet coordinates a symmetric part of the discretized equation dominates over an unsymmetric part in the standard economic environment with low interest rates. It provides some justification for using a fractional step method with implicit treatment of the symmetric part of the weak form of the Black-Scholes operator and with explicit treatment of its unsymmetric part. Then a well-conditioned discrete problem is obtained.

  1. Invariant Discretization Schemes Using Evolution-Projection Techniques

    NASA Astrophysics Data System (ADS)

    Bihlo, Alexander; Nave, Jean-Christophe

    2013-08-01

    Finite difference discretization schemes preserving a subgroup of the maximal Lie invariance group of the one-dimensional linear heat equation are determined. These invariant schemes are constructed using the invariantization procedure for non-invariant schemes of the heat equation in computational coordinates. We propose a new methodology for handling moving discretization grids which are generally indispensable for invariant numerical schemes. The idea is to use the invariant grid equation, which determines the locations of the grid point at the next time level only for a single integration step and then to project the obtained solution to the regular grid using invariant interpolation schemes. This guarantees that the scheme is invariant and allows one to work on the simpler stationary grids. The discretization errors of the invariant schemes are established and their convergence rates are estimated. Numerical tests are carried out to shed some light on the numerical p! roperties of invariant discretization schemes using the proposed evolution-projection strategy.

  2. Discrete-to-continuous transition in quantum phase estimation

    NASA Astrophysics Data System (ADS)

    Rządkowski, Wojciech; Demkowicz-Dobrzański, Rafał

    2017-09-01

    We analyze the problem of quantum phase estimation in which the set of allowed phases forms a discrete N -element subset of the whole [0 ,2 π ] interval, φn=2 π n /N , n =0 ,⋯,N -1 , and study the discrete-to-continuous transition N →∞ for various cost functions as well as the mutual information. We also analyze the relation between the problems of phase discrimination and estimation by considering a step cost function of a given width σ around the true estimated value. We show that in general a direct application of the theory of covariant measurements for a discrete subgroup of the U(1 ) group leads to suboptimal strategies due to an implicit requirement of estimating only the phases that appear in the prior distribution. We develop the theory of subcovariant measurements to remedy this situation and demonstrate truly optimal estimation strategies when performing a transition from discrete to continuous phase estimation.

  3. Discrete Mathematics and Its Applications

    ERIC Educational Resources Information Center

    Oxley, Alan

    2010-01-01

    The article gives ideas that lecturers of undergraduate Discrete Mathematics courses can use in order to make the subject more interesting for students and encourage them to undertake further studies in the subject. It is possible to teach Discrete Mathematics with little or no reference to computing. However, students are more likely to be…

  4. Discrete Mathematics and Its Applications

    ERIC Educational Resources Information Center

    Oxley, Alan

    2010-01-01

    The article gives ideas that lecturers of undergraduate Discrete Mathematics courses can use in order to make the subject more interesting for students and encourage them to undertake further studies in the subject. It is possible to teach Discrete Mathematics with little or no reference to computing. However, students are more likely to be…

  5. Discreteness inducing coexistence

    NASA Astrophysics Data System (ADS)

    dos Santos, Renato Vieira

    2013-12-01

    Consider two species that diffuse through space. Consider further that they differ only in initial densities and, possibly, in diffusion constants. Otherwise they are identical. What happens if they compete with each other in the same environment? What is the influence of the discrete nature of the interactions on the final destination? And what are the influence of diffusion and additive fluctuations corresponding to random migration and immigration of individuals? This paper aims to answer these questions for a particular competition model that incorporates intra and interspecific competition between the species. Based on mean field theory, the model has a stationary state dependent on the initial density conditions. We investigate how this initial density dependence is affected by the presence of demographic multiplicative noise and additive noise in space and time. There are three main conclusions: (1) Additive noise favors denser populations at the expense of the less dense, ratifying the competitive exclusion principle. (2) Demographic noise, on the other hand, favors less dense populations at the expense of the denser ones, inducing equal densities at the quasi-stationary state, violating the aforementioned principle. (3) The slower species always suffers the more deleterious effects of statistical fluctuations in a homogeneous medium.

  6. Uranus - Discrete Cloud

    NASA Technical Reports Server (NTRS)

    1986-01-01

    This false-color Voyager picture of Uranus shows a discrete cloud seen as a bright streak near the planet's limb. The picture is a highly processed composite of three images obtained Jan. 14, 1986, when the spacecraft was 12.9 million kilometers (8.0 million miles) from the planet. The cloud visible here is the most prominent feature seen in a series of Voyager images designed to track atmospheric motions. (The occasional donut-shaped features, including one at the bottom, are shadows cast by dust in the camera optics; the processing necessary to bring out the faint features on the planet also brings out these camera blemishes.) Three separate images were shuttered through violet, blue and orange filters. Each color image showed the cloud to a different degree; because they were not exposed at exactly the same time, the images were processed to provide a correction for a good spatial match. In a true-color image, the cloud would be barely discernible; the false color helps bring out additional details. The different colors imply variations in vertical structure, but as yet is not possible to be specific about such differences. One possibility is that the Uranian atmosphere contains smog-like constituents, in which case some color differences may represent differences in how these molecules are distributed. The Voyager project is managed for NASA by the Jet Propulsion Laboratory.

  7. Quantum search with multiple walk steps per oracle query

    NASA Astrophysics Data System (ADS)

    Wong, Thomas G.; Ambainis, Andris

    2015-08-01

    We identify a key difference between quantum search by discrete- and continuous-time quantum walks: a discrete-time walk typically performs one walk step per oracle query, whereas a continuous-time walk can effectively perform multiple walk steps per query while only counting query time. As a result, we show that continuous-time quantum walks can outperform their discrete-time counterparts, even though both achieve quadratic speedups over their corresponding classical random walks. To provide greater equity, we allow the discrete-time quantum walk to also take multiple walk steps per oracle query while only counting queries. Then it matches the continuous-time algorithm's runtime, but such that it is a cubic speedup over its corresponding classical random walk. This yields a greater-than-quadratic speedup for quantum search over its corresponding classical random walk.

  8. Microscopic derivation of discrete hydrodynamics.

    PubMed

    Español, Pep; Anero, Jesús G; Zúñiga, Ignacio

    2009-12-28

    By using the standard theory of coarse graining based on Zwanzig's projection operator, we derive the dynamic equations for discrete hydrodynamic variables. These hydrodynamic variables are defined in terms of the Delaunay triangulation. The resulting microscopically derived equations can be understood, a posteriori, as a discretization on an arbitrary irregular grid of the Navier-Stokes equations. The microscopic derivation provides a set of discrete equations that exactly conserves mass, momentum, and energy and the dissipative part of the dynamics produces strict entropy increase. In addition, the microscopic derivation provides a practical implementation of thermal fluctuations in a way that the fluctuation-dissipation theorem is satisfied exactly. This paper points toward a close connection between coarse-graining procedures from microscopic dynamics and discretization schemes for partial differential equations.

  9. Chaos in Periodic Discrete Systems

    NASA Astrophysics Data System (ADS)

    Shi, Yuming; Zhang, Lijuan; Yu, Panpan; Huang, Qiuling

    This paper focuses on chaos in periodic discrete systems, whose state space may vary with time. Some close relationships between some chaotic dynamical behaviors of a periodic discrete system and its autonomous induced system are given. Based on these relationships, several criteria of chaos are established and some sufficient conditions for no chaos are given for periodic discrete systems. Further, it is shown that a finite-dimensional linear periodic discrete system is not chaotic in the sense of Li-Yorke or Wiggins. In particular, an interesting problem of whether nonchaotic rules may generate a chaotic system is studied, with some examples provided, one of which surprisingly shows that a composition of globally asymptotically stable maps can be chaotic. In addition, some properties of sign pattern matrices of non-negative square matrices are given for convenience of the study.

  10. Discrete solitons in graphene metamaterials

    NASA Astrophysics Data System (ADS)

    Bludov, Yu. V.; Smirnova, D. A.; Kivshar, Yu. S.; Peres, N. M. R.; Vasilevskiy, M. I.

    2015-01-01

    We study nonlinear properties of multilayer metamaterials created by graphene sheets separated by dielectric layers. We demonstrate that such structures can support localized nonlinear modes described by the discrete nonlinear Schrödinger equation and that its solutions are associated with stable discrete plasmon solitons. We also analyze the nonlinear surface modes in truncated graphene metamaterials being a nonlinear analog of surface Tamm states.

  11. Concurrency and discrete event control

    NASA Technical Reports Server (NTRS)

    Heymann, Michael

    1990-01-01

    Much of discrete event control theory has been developed within the framework of automata and formal languages. An alternative approach inspired by the theories of process-algebra as developed in the computer science literature is presented. The framework, which rests on a new formalism of concurrency, can adequately handle nondeterminism and can be used for analysis of a wide range of discrete event phenomena.

  12. A general gridding, discretization, and coarsening methodology for modeling flow in porous formations with discrete geological features

    NASA Astrophysics Data System (ADS)

    Karimi-Fard, M.; Durlofsky, L. J.

    2016-10-01

    A comprehensive framework for modeling flow in porous media containing thin, discrete features, which could be high-permeability fractures or low-permeability deformation bands, is presented. The key steps of the methodology are mesh generation, fine-grid discretization, upscaling, and coarse-grid discretization. Our specialized gridding technique combines a set of intersecting triangulated surfaces by constructing approximate intersections using existing edges. This procedure creates a conforming mesh of all surfaces, which defines the internal boundaries for the volumetric mesh. The flow equations are discretized on this conforming fine mesh using an optimized two-point flux finite-volume approximation. The resulting discrete model is represented by a list of control-volumes with associated positions and pore-volumes, and a list of cell-to-cell connections with associated transmissibilities. Coarse models are then constructed by the aggregation of fine-grid cells, and the transmissibilities between adjacent coarse cells are obtained using flow-based upscaling procedures. Through appropriate computation of fracture-matrix transmissibilities, a dual-continuum representation is obtained on the coarse scale in regions with connected fracture networks. The fine and coarse discrete models generated within the framework are compatible with any connectivity-based simulator. The applicability of the methodology is illustrated for several two- and three-dimensional examples. In particular, we consider gas production from naturally fractured low-permeability formations, and transport through complex fracture networks. In all cases, highly accurate solutions are obtained with significant model reduction.

  13. Transistor step stress program for JANTX2N4150

    NASA Technical Reports Server (NTRS)

    1979-01-01

    Reliability analysis of the transistor JANTX2N4150 manufactured by General Semiconductor and Transitron is reported. The discrete devices were subjected to power and temperature step stress tests and then to electrical tests after completing the power/temperature step stress point. Control sample units were maintained for verification of the electrical parametric testing. Results are presented.

  14. A space-time discretization procedure for wave propagation problems

    NASA Technical Reports Server (NTRS)

    Davis, Sanford

    1989-01-01

    Higher order compact algorithms are developed for the numerical simulation of wave propagation by using the concept of a discrete dispersion relation. The dispersion relation is the imprint of any linear operator in space-time. The discrete dispersion relation is derived from the continuous dispersion relation by examining the process by which locally plane waves propagate through a chosen grid. The exponential structure of the discrete dispersion relation suggests an efficient splitting of convective and diffusive terms for dissipative waves. Fourth- and eighth-order convection schemes are examined that involve only three or five spatial grid points. These algorithms are subject to the same restrictions that govern the use of dispersion relations in the constructions of asymptotic expansions to nonlinear evolution equations. A new eighth-order scheme is developed that is exact for Courant numbers of 1, 2, 3, and 4. Examples are given of a pulse and step wave with a small amount of physical diffusion.

  15. Stability analysis of the Euler discretization for SIR epidemic model

    SciTech Connect

    Suryanto, Agus

    2014-06-19

    In this paper we consider a discrete SIR epidemic model obtained by the Euler method. For that discrete model, existence of disease free equilibrium and endemic equilibrium is established. Sufficient conditions on the local asymptotical stability of both disease free equilibrium and endemic equilibrium are also derived. It is found that the local asymptotical stability of the existing equilibrium is achieved only for a small time step size h. If h is further increased and passes the critical value, then both equilibriums will lose their stability. Our numerical simulations show that a complex dynamical behavior such as bifurcation or chaos phenomenon will appear for relatively large h. Both analytical and numerical results show that the discrete SIR model has a richer dynamical behavior than its continuous counterpart.

  16. Supervised and unsupervised discretization methods for evolutionary algorithms

    SciTech Connect

    Cantu-Paz, E

    2001-01-24

    This paper introduces simple model-building evolutionary algorithms (EAs) that operate on continuous domains. The algorithms are based on supervised and unsupervised discretization methods that have been used as preprocessing steps in machine learning. The basic idea is to discretize the continuous variables and use the discretization as a simple model of the solutions under consideration. The model is then used to generate new solutions directly, instead of using the usual operators based on sexual recombination and mutation. The algorithms presented here have fewer parameters than traditional and other model-building EAs. They expect that the proposed algorithms that use multivariate models scale up better to the dimensionality of the problem than existing EAs.

  17. Quantum walks and non-Abelian discrete gauge theory

    NASA Astrophysics Data System (ADS)

    Arnault, Pablo; Di Molfetta, Giuseppe; Brachet, Marc; Debbasch, Fabrice

    2016-07-01

    A family of discrete-time quantum walks (DTQWs) on the line with an exact discrete U(N ) gauge invariance is introduced. It is shown that the continuous limit of these DTQWs, when it exists, coincides with the dynamics of a Dirac fermion coupled to usual U(N ) gauge fields in two-dimensional spacetime. A discrete generalization of the usual U(N ) curvature is also constructed. An alternate interpretation of these results in terms of superimposed U(1 ) Maxwell fields and SU(N ) gauge fields is discussed in the Appendix. Numerical simulations are also presented, which explore the convergence of the DTQWs towards their continuous limit and which also compare the DTQWs with classical (i.e., nonquantum) motions in classical SU(2 ) fields. The results presented in this paper constitute a first step towards quantum simulations of generic Yang-Mills gauge theories through DTQWs.

  18. Distributed Relaxation for Conservative Discretizations

    NASA Technical Reports Server (NTRS)

    Diskin, Boris; Thomas, James L.

    2001-01-01

    A multigrid method is defined as having textbook multigrid efficiency (TME) if the solutions to the governing system of equations are attained in a computational work that is a small (less than 10) multiple of the operation count in one target-grid residual evaluation. The way to achieve this efficiency is the distributed relaxation approach. TME solvers employing distributed relaxation have already been demonstrated for nonconservative formulations of high-Reynolds-number viscous incompressible and subsonic compressible flow regimes. The purpose of this paper is to provide foundations for applications of distributed relaxation to conservative discretizations. A direct correspondence between the primitive variable interpolations for calculating fluxes in conservative finite-volume discretizations and stencils of the discretized derivatives in the nonconservative formulation has been established. Based on this correspondence, one can arrive at a conservative discretization which is very efficiently solved with a nonconservative relaxation scheme and this is demonstrated for conservative discretization of the quasi one-dimensional Euler equations. Formulations for both staggered and collocated grid arrangements are considered and extensions of the general procedure to multiple dimensions are discussed.

  19. Rumor Processes on and Discrete Renewal Processes

    NASA Astrophysics Data System (ADS)

    Gallo, Sandro; Garcia, Nancy L.; Junior, Valdivino Vargas; Rodríguez, Pablo M.

    2014-05-01

    We study two rumor processes on , the dynamics of which are related to an SI epidemic model with long range transmission. Both models start with one spreader at site and ignorants at all the other sites of , but differ by the transmission mechanism. In one model, the spreaders transmit the information within a random distance on their right, and in the other the ignorants take the information from a spreader within a random distance on their left. We obtain the probability of survival, information on the distribution of the range of the rumor and limit theorems for the proportion of spreaders. The key step of our proofs is to show that, in each model, the position of the spreaders on can be related to a suitably chosen discrete renewal process.

  20. Minisuperspace models of discrete systems

    NASA Astrophysics Data System (ADS)

    Baytaş, Bekir; Bojowald, Martin

    2017-04-01

    A discrete quantum spin system is presented in which several modern methods of canonical quantum gravity can be tested with promising results. In particular, features of interacting dynamics are analyzed with an emphasis on homogeneous configurations and the dynamical building-up and stability of long-range correlations. Different types of homogeneous minisuperspace models are introduced for the system, including one based on condensate states, and shown to capture different aspects of the discrete system. They are evaluated with effective methods and by means of continuum limits, showing good agreement with operator calculations whenever the latter are available. As a possibly quite general result, it is concluded that an analysis of the building-up of long-range correlations in discrete systems requires nonperturbative solutions of the dynamical equations. Some questions related to stability can be analyzed perturbatively but suggest that matter couplings may be relevant for this question in the context of quantum cosmology.

  1. Discretization errors in particle tracking

    NASA Astrophysics Data System (ADS)

    Carmon, G.; Mamman, N.; Feingold, M.

    2007-03-01

    High precision video tracking of microscopic particles is limited by systematic and random errors. Systematic errors are partly due to the discretization process both in position and in intensity. We study the behavior of such errors in a simple tracking algorithm designed for the case of symmetric particles. This symmetry algorithm uses interpolation to estimate the value of the intensity at arbitrary points in the image plane. We show that the discretization error is composed of two parts: (1) the error due to the discretization of the intensity, bD and (2) that due to interpolation, bI. While bD behaves asymptotically like N-1 where N is the number of intensity gray levels, bI is small when using cubic spline interpolation.

  2. Integrable structure in discrete shell membrane theory

    PubMed Central

    Schief, W. K.

    2014-01-01

    We present natural discrete analogues of two integrable classes of shell membranes. By construction, these discrete shell membranes are in equilibrium with respect to suitably chosen internal stresses and external forces. The integrability of the underlying equilibrium equations is proved by relating the geometry of the discrete shell membranes to discrete O surface theory. We establish connections with generalized barycentric coordinates and nine-point centres and identify a discrete version of the classical Gauss equation of surface theory. PMID:24808755

  3. On the Importance of the Dynamics of Discretizations

    NASA Technical Reports Server (NTRS)

    Sweby, Peter K.; Yee, H. C.; Rai, ManMohan (Technical Monitor)

    1995-01-01

    It has been realized recently that the discrete maps resulting from numerical discretizations of differential equations can possess asymptotic dynamical behavior quite different from that of the original systems. This is the case not only for systems of Ordinary Differential Equations (ODEs) but in a more complicated manner for Partial Differential Equations (PDEs) used to model complex physics. The impact of the modified dynamics may be mild and even not observed for some numerical methods. For other classes of discretizations the impact may be pronounced, but not always obvious depending on the nonlinear model equations, the time steps, the grid spacings and the initial conditions. Non-convergence or convergence to periodic solutions might be easily recognizable but convergence to incorrect but plausible solutions may not be so obvious - even for discretized parameters within the linearized stability constraint. Based on our past four years of research, we will illustrate some of the pathology of the dynamics of discretizations, its possible impact and the usage of these schemes for model nonlinear ODEs, convection-diffusion equations and grid adaptations.

  4. On the Importance of the Dynamics of Discretizations

    NASA Technical Reports Server (NTRS)

    Sweby, Peter K.; Yee, H. C.; Rai, ManMohan (Technical Monitor)

    1995-01-01

    It has been realized recently that the discrete maps resulting from numerical discretizations of differential equations can possess asymptotic dynamical behavior quite different from that of the original systems. This is the case not only for systems of Ordinary Differential Equations (ODEs) but in a more complicated manner for Partial Differential Equations (PDEs) used to model complex physics. The impact of the modified dynamics may be mild and even not observed for some numerical methods. For other classes of discretizations the impact may be pronounced, but not always obvious depending on the nonlinear model equations, the time steps, the grid spacings and the initial conditions. Non-convergence or convergence to periodic solutions might be easily recognizable but convergence to incorrect but plausible solutions may not be so obvious - even for discretized parameters within the linearized stability constraint. Based on our past four years of research, we will illustrate some of the pathology of the dynamics of discretizations, its possible impact and the usage of these schemes for model nonlinear ODEs, convection-diffusion equations and grid adaptations.

  5. Stable discrete surface light bullets.

    PubMed

    Mihalache, Dumitru; Mazilu, Dumitru; Lederer, Falk; Kivshar, Yuri S

    2007-01-22

    We analyze spatiotemporal light localization near the edge of a semi-infinite array of weakly coupled nonlinear optical waveguides and demonstrate the existence of a novel class of continuous-discrete spatiotemporal solitons, the so-called discrete surface light bullets. We show that their properties are strongly affected by the presence of the surface. To this end the crossover between surface and quasi-bulk bullets is studied by analyzing the families of solitons propagating at different distances from the edge of the waveguide array.

  6. Discrete cloud structure on Neptune

    NASA Technical Reports Server (NTRS)

    Hammel, H. B.

    1989-01-01

    Recent CCD imaging data for the discrete cloud structure of Neptune shows that while cloud features at CH4-band wavelengths are manifest in the southern hemisphere, they have not been encountered in the northern hemisphere since 1986. A literature search has shown the reflected CH4-band light from the planet to have come from a single discrete feature at least twice in the last 10 years. Disk-integrated photometry derived from the imaging has demonstrated that a bright cloud feature was responsible for the observed 8900 A diurnal variation in 1986 and 1987.

  7. Discontinuous Galerkin Methods and Local Time Stepping for Wave Propagation

    SciTech Connect

    Grote, M. J.; Mitkova, T.

    2010-09-30

    Locally refined meshes impose severe stability constraints on explicit time-stepping methods for the numerical simulation of time dependent wave phenomena. To overcome that stability restriction, local time-stepping methods are developed, which allow arbitrarily small time steps precisely where small elements in the mesh are located. When combined with a discontinuous Galerkin finite element discretization in space, which inherently leads to a diagonal mass matrix, the resulting numerical schemes are fully explicit. Starting from the classical Adams-Bashforth multi-step methods, local time stepping schemes of arbitrarily high accuracy are derived. Numerical experiments validate the theory and illustrate the usefulness of the proposed time integration schemes.

  8. Review of finite fields: Applications to discrete Fourier, transforms and Reed-Solomon coding

    NASA Technical Reports Server (NTRS)

    Wong, J. S. L.; Truong, T. K.; Benjauthrit, B.; Mulhall, B. D. L.; Reed, I. S.

    1977-01-01

    An attempt is made to provide a step-by-step approach to the subject of finite fields. Rigorous proofs and highly theoretical materials are avoided. The simple concepts of groups, rings, and fields are discussed and developed more or less heuristically. Examples are used liberally to illustrate the meaning of definitions and theories. Applications include discrete Fourier transforms and Reed-Solomon coding.

  9. Optimal time step for incompressible SPH

    NASA Astrophysics Data System (ADS)

    Violeau, Damien; Leroy, Agnès

    2015-05-01

    A classical incompressible algorithm for Smoothed Particle Hydrodynamics (ISPH) is analyzed in terms of critical time step for numerical stability. For this purpose, a theoretical linear stability analysis is conducted for unbounded homogeneous flows, leading to an analytical formula for the maximum CFL (Courant-Friedrichs-Lewy) number as a function of the Fourier number. This gives the maximum time step as a function of the fluid viscosity, the flow velocity scale and the SPH discretization size (kernel standard deviation). Importantly, the maximum CFL number at large Reynolds number appears twice smaller than with the traditional Weakly Compressible (WCSPH) approach. As a consequence, the optimal time step for ISPH is only five times larger than with WCSPH. The theory agrees very well with numerical data for two usual kernels in a 2-D periodic flow. On the other hand, numerical experiments in a plane Poiseuille flow show that the theory overestimates the maximum allowed time step for small Reynolds numbers.

  10. Detection of Steps in Single Molecule Data

    PubMed Central

    Aggarwal, Tanuj; Materassi, Donatello; Davison, Robert; Hays, Thomas; Salapaka, Murti

    2013-01-01

    Over the past few decades, single molecule investigations employing optical tweezers, AFM and TIRF microscopy have revealed that molecular behaviors are typically characterized by discrete steps or events that follow changes in protein conformation. These events, that manifest as steps or jumps, are short-lived transitions between otherwise more stable molecular states. A major limiting factor in determining the size and timing of the steps is the noise introduced by the measurement system. To address this impediment to the analysis of single molecule behaviors, step detection algorithms incorporate large records of data and provide objective analysis. However, existing algorithms are mostly based on heuristics that are not reliable and lack objectivity. Most of these step detection methods require the user to supply parameters that inform the search for steps. They work well, only when the signal to noise ratio (SNR) is high and stepping speed is low. In this report, we have developed a novel step detection method that performs an objective analysis on the data without input parameters, and based only on the noise statistics. The noise levels and characteristics can be estimated from the data providing reliable results for much smaller SNR and higher stepping speeds. An iterative learning process drives the optimization of step-size distributions for data that has unimodal step-size distribution, and produces extremely low false positive outcomes and high accuracy in finding true steps. Our novel methodology, also uniquely incorporates compensation for the smoothing affects of probe dynamics. A mechanical measurement probe typically takes a finite time to respond to step changes, and when steps occur faster than the probe response time, the sharp step transitions are smoothed out and can obscure the step events. To address probe dynamics we accept a model for the dynamic behavior of the probe and invert it to reveal the steps. No other existing method addresses

  11. Discrete transparent boundary conditions for Schroedinger-type equations

    SciTech Connect

    Schmidt, F.; Yevick, D.

    1997-06-01

    We present a general technique for constructing nonlocal transparent boundary conditions for one-dimensional Schroedinger-type equations. Our method supplies boundary conditions for the {theta}-family of implicit one-step discretizations of Schroedinger`s equation in time. The use of Mikusinski`s operator approach in time avoids direct and inverse transforms between time and frequency domains and thus implements the boundary conditions in a direct manner. 14 refs., 9 figs.

  12. Police Discretion: A Selected Bibliography.

    ERIC Educational Resources Information Center

    Brenner, Robert N.; Kravitz, Marjorie

    This bibliography was compiled with two goals. The first goal is to provide police administrators and officers with an overview of the issues involved in developing guidelines for police discretion and a discussion of the options available. The second goal is to demonstrate the need for continuing dialogue and interaction between lawmakers, law…

  13. Professional Discretion and Teacher Satisfaction.

    ERIC Educational Resources Information Center

    Sweeney, Jim

    1981-01-01

    Reports a survey of 1,295 teachers in large Iowa high schools on their needs (following Maslow's categories) in relation to age, sex, and student ability level taught, plus their overall job satisfaction and its relationship to their professional discretion, participation in decision making, and reciprocal trust. (Author/SJL)

  14. Discrete tomography in neutron radiography

    NASA Astrophysics Data System (ADS)

    Kuba, Attila; Rodek, Lajos; Kiss, Zoltán; Ruskó, László; Nagy, Antal; Balaskó, Márton

    2005-04-01

    Discrete tomography (DT) is an imaging technique for reconstructing discrete images from their projections using the knowledge that the object to be reconstructed contains only a few homogeneous materials characterized by known discrete absorption values. One of the main reasons for applying DT is that we will hopefully require relatively few projections. Using discreteness and some a priori information (such as an approximate shape of the object) we can apply two DT methods in neutron imaging by reducing the problem to an optimization task. The first method is a special one because it is only suitable if the object is composed of cylinders and sphere shapes. The second method is a general one in the sense that it can be used for reconstructing objects of any shape. Software was developed and physical experiments performed in order to investigate the effects of several reconstruction parameters: the number of projections, noise levels, and complexity of the object to be reconstructed. We give a summary of the experimental results and make a comparison of the results obtained using a classical reconstruction technique (FBP). The programs we developed are available in our DT reconstruction program package DIRECT.

  15. Discrete implementations of scale transform

    NASA Astrophysics Data System (ADS)

    Djurdjanovic, Dragan; Williams, William J.; Koh, Christopher K.

    1999-11-01

    Scale as a physical quantity is a recently developed concept. The scale transform can be viewed as a special case of the more general Mellin transform and its mathematical properties are very applicable in the analysis and interpretation of the signals subject to scale changes. A number of single-dimensional applications of scale concept have been made in speech analysis, processing of biological signals, machine vibration analysis and other areas. Recently, the scale transform was also applied in multi-dimensional signal processing and used for image filtering and denoising. Discrete implementation of the scale transform can be carried out using logarithmic sampling and the well-known fast Fourier transform. Nevertheless, in the case of the uniformly sampled signals, this implementation involves resampling. An algorithm not involving resampling of the uniformly sampled signals has been derived too. In this paper, a modification of the later algorithm for discrete implementation of the direct scale transform is presented. In addition, similar concept was used to improve a recently introduced discrete implementation of the inverse scale transform. Estimation of the absolute discretization errors showed that the modified algorithms have a desirable property of yielding a smaller region of possible error magnitudes. Experimental results are obtained using artificial signals as well as signals evoked from the temporomandibular joint. In addition, discrete implementations for the separable two-dimensional direct and inverse scale transforms are derived. Experiments with image restoration and scaling through two-dimensional scale domain using the novel implementation of the separable two-dimensional scale transform pair are presented.

  16. Step-Growth Polymerization.

    ERIC Educational Resources Information Center

    Stille, J. K.

    1981-01-01

    Following a comparison of chain-growth and step-growth polymerization, focuses on the latter process by describing requirements for high molecular weight, step-growth polymerization kinetics, synthesis and molecular weight distribution of some linear step-growth polymers, and three-dimensional network step-growth polymers. (JN)

  17. Stepping motor controller

    DOEpatents

    Bourret, S.C.; Swansen, J.E.

    1982-07-02

    A stepping motor is microprocessor controlled by digital circuitry which monitors the output of a shaft encoder adjustably secured to the stepping motor and generates a subsequent stepping pulse only after the preceding step has occurred and a fixed delay has expired. The fixed delay is variable on a real-time basis to provide for smooth and controlled deceleration.

  18. Stepping motor controller

    DOEpatents

    Bourret, Steven C.; Swansen, James E.

    1984-01-01

    A stepping motor is microprocessingly controlled by digital circuitry which monitors the output of a shaft encoder adjustably secured to the stepping motor and generates a subsequent stepping pulse only after the preceding step has occurred and a fixed delay has expired. The fixed delay is variable on a real-time basis to provide for smooth and controlled deceleration.

  19. Discrete gauge symmetries in discrete MSSM-like orientifolds

    NASA Astrophysics Data System (ADS)

    Ibáñez, L. E.; Schellekens, A. N.; Uranga, A. M.

    2012-12-01

    Motivated by the necessity of discrete ZN symmetries in the MSSM to insure baryon stability, we study the origin of discrete gauge symmetries from open string sector U(1)'s in orientifolds based on rational conformal field theory. By means of an explicit construction, we find an integral basis for the couplings of axions and U(1) factors for all simple current MIPFs and orientifolds of all 168 Gepner models, a total of 32 990 distinct cases. We discuss how the presence of discrete symmetries surviving as a subgroup of broken U(1)'s can be derived using this basis. We apply this procedure to models with MSSM chiral spectrum, concretely to all known U(3)×U(2)×U(1)×U(1) and U(3)×Sp(2)×U(1)×U(1) configurations with chiral bi-fundamentals, but no chiral tensors, as well as some SU(5) GUT models. We find examples of models with Z2 (R-parity) and Z3 symmetries that forbid certain B and/or L violating MSSM couplings. Their presence is however relatively rare, at the level of a few percent of all cases.

  20. Stability analysis of implicit time discretizations for the Compton-scattering Fokker-Planck equation

    SciTech Connect

    Densmore, Jeffery D; Warsa, James S; Lowrie, Robert B; Morel, Jim E

    2008-01-01

    The Fokker-Planck equation is a widely used approximation for modeling the Compton scattering of photons in high energy density applications. In this paper, we perform a stability analysis of three implicit time discretizations for the Compton-Scattering Fokker-Planck equation. Specifically, we examine (i) a Semi-Implicit (SI) scheme that employs backward-Euler differencing but evaluates temperature-dependent coefficients at their beginning-of-time-step values, (ii) a Fully Implicit (FI) discretization that instead evaluates temperature-dependent coefficients at their end-of-time-step values, and (iii) a Linearized Implicit (LI) scheme, which is developed by linearizing the temperature dependence of the FI discretization within each time step. Our stability analysis shows that the FI and LI schemes are unconditionally stable and cannot generate oscillatory solutions regardless of time-step size, whereas the SI discretization can suffer from instabilities and nonphysical oscillations for sufficiently large time steps. With the results of this analysis, we present time-step limits for the SI scheme that prevent undesirable behavior. We test the validity of our stability analysis and time-step limits with a set of numerical examples.

  1. Discrete-time quantum walk with feed-forward quantum coin

    PubMed Central

    Shikano, Yutaka; Wada, Tatsuaki; Horikawa, Junsei

    2014-01-01

    Constructing a discrete model like a cellular automaton is a powerful method for understanding various dynamical systems. However, the relationship between the discrete model and its continuous analogue is, in general, nontrivial. As a quantum-mechanical cellular automaton, a discrete-time quantum walk is defined to include various quantum dynamical behavior. Here we generalize a discrete-time quantum walk on a line into the feed-forward quantum coin model, which depends on the coin state of the previous step. We show that our proposed model has an anomalous slow diffusion characterized by the porous-medium equation, while the conventional discrete-time quantum walk model shows ballistic transport. PMID:24651053

  2. A road to practical dielectric elastomer actuators based robotics and mechatronics: discrete actuation

    NASA Astrophysics Data System (ADS)

    Plante, Jean-Sébastien; Devita, Lauren M.; Dubowsky, Steven

    2007-04-01

    Fundamental studies of Dielectric Elastomer Actuators (DEAs) using viscoelastic materials such as VHB 4905/4910 from 3M showed significant advantages at high stretch rates. The film's viscous forces increase actuator life and the short power-on times minimize energy losses through current leakage. This paper presents a design paradigm that exploits these fundamental properties of DEAs called discrete actuation. Discrete actuation uses DEAs at high stretch rates to change the states of robotic or mechatronic systems in discrete steps. Each state of the system is stable and can be maintained without actuator power. Discrete actuation can be used in robotic and mechatronic applications such as manipulation and locomotion. The resolution of such systems increases with the number of discrete states, 10 to 100 being sufficient for many applications. An MRI-guided needle positioning device for cancer treatments and a space exploration robot using hopping for locomotion are presented as examples of this concept.

  3. Spatial data discretization methods for geocomputation

    NASA Astrophysics Data System (ADS)

    Cao, Feng; Ge, Yong; Wang, Jinfeng

    2014-02-01

    Geocomputation provides solutions to complex geographic problems. Continuous and discrete spatial data are involved in the geocomputational process; however, geocomputational methods for discrete spatial data cannot be directly applied to continuous or mixed spatial data. Therefore, discretization methods for continuous or mixed spatial data are involved in the process. Since spatial data has spatial features, such as association, heterogeneity and spatial structure, these features cannot be handled by traditional discretization methods. Therefore, this work develops feature-based spatial data discretization methods that achieve optimal discretization results for spatial data using spatial information implicit in those features. Two discretization methods considering the features of spatial data are presented. One is an unsupervised method considering autocorrelation of spatial data and the other is a supervised method considering spatial heterogeneity. Discretization processes of the two methods are exemplified using neural tube defects (NTD) for Heshun County in Shanxi Province, China. Effectiveness is also assessed.

  4. Systoles in discrete dynamical systems

    NASA Astrophysics Data System (ADS)

    Fernandes, Sara; Grácio, Clara; Ramos, Carlos Correia

    2013-01-01

    The fruitful relationship between Geometry and Graph Theory has been explored by several authors benefiting also the Theory of discrete dynamical systems seen as Markov chains in graphs. In this work we will further explore the relation between these areas, giving a geometrical interpretation of notions from dynamical systems. In particular, we relate the topological entropy with the systole, here defined in the context of discrete dynamical systems. We show that for continuous interval maps the systole is trivial; however, for the class of interval maps with one discontinuity point the systole acquires relevance from the point of view of the dynamical behavior. Moreover, we define the geodesic length spectrum associated to a Markov interval map and we compute the referred spectrum in several examples.

  5. Dark energy from discrete spacetime.

    PubMed

    Trout, Aaron D

    2013-01-01

    Dark energy accounts for most of the matter-energy content of our universe, yet current theories of its origin rely on radical physical assumptions such as the holographic principle or controversial anthropic arguments. We give a better motivated explanation for dark energy, claiming that it arises from a small negative scalar-curvature present even in empty spacetime. The vacuum has this curvature because spacetime is fundamentally discrete and there are more ways for a discrete geometry to have negative curvature than positive. We explicitly compute this effect using a variant of the well known dynamical-triangulations (DT) model for quantum gravity. Our model predicts a time-varying non-zero cosmological constant with a current value, [Formula: see text] in natural units, in agreement with observation. This calculation is made possible by a novel characterization of the possible DT action values combined with numerical evidence concerning their degeneracies.

  6. Dark Energy from Discrete Spacetime

    PubMed Central

    Trout, Aaron D.

    2013-01-01

    Dark energy accounts for most of the matter-energy content of our universe, yet current theories of its origin rely on radical physical assumptions such as the holographic principle or controversial anthropic arguments. We give a better motivated explanation for dark energy, claiming that it arises from a small negative scalar-curvature present even in empty spacetime. The vacuum has this curvature because spacetime is fundamentally discrete and there are more ways for a discrete geometry to have negative curvature than positive. We explicitly compute this effect using a variant of the well known dynamical-triangulations (DT) model for quantum gravity. Our model predicts a time-varying non-zero cosmological constant with a current value, in natural units, in agreement with observation. This calculation is made possible by a novel characterization of the possible DT action values combined with numerical evidence concerning their degeneracies. PMID:24312502

  7. Immersed Boundary Fractional Step Method

    NASA Astrophysics Data System (ADS)

    Taira, Kunihiko

    2005-11-01

    We present a new formulation of the immersed boundary method for incompressible flow over moving rigid bodies. Like many existing techniques we introduce a set of interpolation points on the surface at which the no-slip boundary condition is satisfied by including a (regularized) force in the momentum equations. By introducing interpolation and regularization operators and grouping pressure and force unknowns together, the discretized Navier-Stokes equations with the immersed boundary method can be formulated with an identical structure to the traditional fractional step method, but with a modified Poisson equation whose unknowns are both the pressure and the boundary force. The method highlights the analogous roles of pressure and boundary forcing as Lagrange multipliers in order to satisfy the divergence free and no-slip constraints, respectively. The overall method is found to be a simple addition to an existing fractional step code and the extended Poisson equation is solved efficiently with the conjugate gradient method. We demonstrate convergence and present results for two-dimensional flows with a variety of moving rigid bodies.

  8. Observability of discretized partial differential equations

    NASA Technical Reports Server (NTRS)

    Cohn, Stephen E.; Dee, Dick P.

    1988-01-01

    It is shown that complete observability of the discrete model used to assimilate data from a linear partial differential equation (PDE) system is necessary and sufficient for asymptotic stability of the data assimilation process. The observability theory for discrete systems is reviewed and applied to obtain simple observability tests for discretized constant-coefficient PDEs. Examples are used to show how numerical dispersion can result in discrete dynamics with multiple eigenvalues, thereby detracting from observability.

  9. Step by Step: Avoiding Spiritual Bypass in 12-Step Work

    ERIC Educational Resources Information Center

    Cashwell, Craig S.; Clarke, Philip B.; Graves, Elizabeth G.

    2009-01-01

    With spirituality as a cornerstone, 12-step groups serve a vital role in the recovery community. It is important for counselors to be mindful, however, of the potential for clients to be in spiritual bypass, which likely will undermine the recovery process.

  10. Interference in discrete Wigner functions

    SciTech Connect

    Cormick, Cecilia; Paz, Juan Pablo

    2006-12-15

    We analyze some features of the class of discrete Wigner functions that was recently introduced by Gibbons et al. [Phys. Rev. A 70, 062101 (2004)] to represent quantum states of systems with power-of-prime dimensional Hilbert spaces. We consider ''cat'' states obtained as coherent superpositions of states with positive Wigner function; for such states we show that the oscillations of the discrete Wigner function typically spread over the entire discrete phase space (including the regions where the two interfering states are localized). This is a generic property, which is in sharp contrast with the usual attributes of Wigner functions that make them useful candidates to display the existence of quantum coherence through oscillations. However, it is possible to find subsets of cat states with a natural phase-space representation, in which the oscillatory regions remain localized. We show that this can be done for interesting families of stabilizer states used in quantum error-correcting codes, and illustrate this by analyzing the phase-space representation of the five-qubit error-correcting code.

  11. Discrete Proportional Plus Integral (PI) Multivariable Control Laws for the Control Reconfigurable Combat Aircraft (CRCA)

    DTIC Science & Technology

    1989-12-01

    and perform the maneuvers. The time responses show that discrete PI control law can make the CRCA successfully perform all five maneuvers for two of the...three control surface failures investigated in two of the three point designs. The step response PI control law results in stable control for only...tasks with the NASA/Grumman Control Reconfigurable Combat Aircraft (CRCA). Porter’s method is used to design discrete Proportional plus Integral ( PI

  12. Umbral Deformations on Discrete SPACE TIME

    NASA Astrophysics Data System (ADS)

    Zachos, Cosmas K.

    Given a minimum measurable length underlying spacetime, the latter may be effectively regarded as discrete, at scales of order the Planck length. A systematic discretization of continuum physics may be effected most efficiently through the umbral deformation. General functionals yielding such deformations at the level of solutions are furnished and illustrated, and broad features of discrete oscillations and wave propagation are outlined.

  13. Discrete Optimization in Chemical Space Reference Manual

    DTIC Science & Technology

    2012-10-01

    Discrete Optimization in Chemical Space Reference Manual by B. C. Rinderspacher ARL-TR-6202 October 2012...2012 Discrete Optimization in Chemical Space Reference Manual B. C. Rinderspacher Weapons and Materials Research Directorate, ARL...2011 4. TITLE AND SUBTITLE Discrete Optimization in Chemical Space Reference Manual 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT

  14. A Step Circuit Program.

    ERIC Educational Resources Information Center

    Herman, Susan

    1995-01-01

    Aerobics instructors can use step aerobics to motivate students. One creative method is to add the step to the circuit workout. By incorporating the step, aerobic instructors can accommodate various fitness levels. The article explains necessary equipment and procedures, describing sample stations for cardiorespiratory fitness, muscular strength,…

  15. A Step Circuit Program.

    ERIC Educational Resources Information Center

    Herman, Susan

    1995-01-01

    Aerobics instructors can use step aerobics to motivate students. One creative method is to add the step to the circuit workout. By incorporating the step, aerobic instructors can accommodate various fitness levels. The article explains necessary equipment and procedures, describing sample stations for cardiorespiratory fitness, muscular strength,…

  16. Graphics processing unit parallel accelerated solution of the discrete ordinates for photon transport in biological tissues.

    PubMed

    Peng, Kuan; Gao, Xinbo; Qu, Xiaochao; Ren, Nunu; Chen, Xueli; He, Xiaowei; Wang, Xiaorei; Liang, Jimin; Tian, Jie

    2011-07-20

    As a widely used numerical solution for the radiation transport equation (RTE), the discrete ordinates can predict the propagation of photons through biological tissues more accurately relative to the diffusion equation. The discrete ordinates reduce the RTE to a serial of differential equations that can be solved by source iteration (SI). However, the tremendous time consumption of SI, which is partly caused by the expensive computation of each SI step, limits its applications. In this paper, we present a graphics processing unit (GPU) parallel accelerated SI method for discrete ordinates. Utilizing the calculation independence on the levels of the discrete ordinate equation and spatial element, the proposed method reduces the time cost of each SI step by parallel calculation. The photon reflection at the boundary was calculated based on the results of the last SI step to ensure the calculation independence on the level of the discrete ordinate equation. An element sweeping strategy was proposed to detect the calculation independence on the level of the spatial element. A GPU parallel frame called the compute unified device architecture was employed to carry out the parallel computation. The simulation experiments, which were carried out with a cylindrical phantom and numerical mouse, indicated that the time cost of each SI step can be reduced up to a factor of 228 by the proposed method with a GTX 260 graphics card. © 2011 Optical Society of America

  17. Discrete Boltzmann equation for microfluidics.

    PubMed

    Li, Baoming; Kwok, Daniel Y

    2003-03-28

    We propose a discrete Boltzmann model for microfluidics based on the Boltzmann equation with external forces using a single relaxation time collision model. Considering the electrostatic interactions in microfluidics systems, we introduce an equilibrium distribution function that differs from the Maxwell-Boltzmann distribution by an exponential factor to represent the action of an external force field. A statistical mechanical approach is applied to derive the equivalent external acceleration force exerting on the lattice particles based on a mean-field approximation, resulting from the electro-static potential energy and intermolecular potential energy between fluid-fluid and fluid-substrate interactions.

  18. Invariants of broken discrete symmetries.

    PubMed

    Kalozoumis, P A; Morfonios, C; Diakonos, F K; Schmelcher, P

    2014-08-01

    The parity and Bloch theorems are generalized to the case of broken global symmetry. Local inversion or translation symmetries in one dimension are shown to yield invariant currents that characterize wave propagation. These currents map the wave function from an arbitrary spatial domain to any symmetry-related domain. Our approach addresses any combination of local symmetries, thus applying, in particular, to acoustic, optical, and matter waves. Nonvanishing values of the invariant currents provide a systematic pathway to the breaking of discrete global symmetries.

  19. Invariants of Broken Discrete Symmetries

    NASA Astrophysics Data System (ADS)

    Kalozoumis, P. A.; Morfonios, C.; Diakonos, F. K.; Schmelcher, P.

    2014-08-01

    The parity and Bloch theorems are generalized to the case of broken global symmetry. Local inversion or translation symmetries in one dimension are shown to yield invariant currents that characterize wave propagation. These currents map the wave function from an arbitrary spatial domain to any symmetry-related domain. Our approach addresses any combination of local symmetries, thus applying, in particular, to acoustic, optical, and matter waves. Nonvanishing values of the invariant currents provide a systematic pathway to the breaking of discrete global symmetries.

  20. Directed self-assembly of proteins into discrete radial patterns

    PubMed Central

    Thakur, Garima; Prashanthi, Kovur; Thundat, Thomas

    2013-01-01

    Unlike physical patterning of materials at nanometer scale, manipulating soft matter such as biomolecules into patterns is still in its infancy. Self-assembled monolayer (SAM) with surface density gradient has the capability to drive biomolecules in specific directions to create hierarchical and discrete structures. Here, we report on a two-step process of self-assembly of the human serum albumin (HSA) protein into discrete ring structures based on density gradient of SAM. The methodology involves first creating a 2-dimensional (2D) polyethylene glycol (PEG) islands with responsive carboxyl functionalities. Incubation of proteins on such pre-patterned surfaces results in direct self-assembly of protein molecules around PEG islands. Immobilization and adsorption of protein on such structures over time evolve into the self-assembled patterns. PMID:23719678

  1. Cyclic steps on ice

    NASA Astrophysics Data System (ADS)

    Yokokawa, M.; Izumi, N.; Naito, K.; Parker, G.; Yamada, T.; Greve, R.

    2016-05-01

    Boundary waves often form at the interface between ice and fluid flowing adjacent to it, such as ripples under river ice covers, and steps on the bed of supraglacial meltwater channels. They may also be formed by wind, such as the megadunes on the Antarctic ice sheet. Spiral troughs on the polar ice caps of Mars have been interpreted to be cyclic steps formed by katabatic wind blowing over ice. Cyclic steps are relatives of upstream-migrating antidunes. Cyclic step formation on ice is not only a mechanical but also a thermodynamic process. There have been very few studies on the formation of either cyclic steps or upstream-migrating antidunes on ice. In this study, we performed flume experiments to reproduce cyclic steps on ice by flowing water, and found that trains of steps form when the Froude number is larger than unity. The features of those steps allow them to be identified as ice-bed analogs of cyclic steps in alluvial and bedrock rivers. We performed a linear stability analysis and obtained a physical explanation of the formation of upstream-migrating antidunes, i.e., precursors of cyclic steps. We compared the results of experiments with the predictions of the analysis and found the observed steps fall in the range where the analysis predicts interfacial instability. We also found that short antidune-like undulations formed as a precursor to the appearance of well-defined steps. This fact suggests that such antidune-like undulations correspond to the instability predicted by the analysis and are precursors of cyclic steps.

  2. Entwinement in discretely gauged theories

    NASA Astrophysics Data System (ADS)

    Balasubramanian, V.; Bernamonti, A.; Craps, B.; De Jonckheere, T.; Galli, F.

    2016-12-01

    We develop the notion of "entwinement" to characterize the amount of quantum entanglement between internal, discretely gauged degrees of freedom in a quantum field theory. This concept originated in the program of reconstructing spacetime from entanglement in holographic duality. We define entwinement formally in terms of a novel replica method which uses twist operators charged in a representation of the discrete gauge group. In terms of these twist operators we define a non-local, gauge-invariant object whose expectation value computes entwinement in a standard replica limit. We apply our method to the computation of entwinement in symmetric orbifold conformal field theories in 1+1 dimensions, which have an S N gauging. Such a theory appears in the weak coupling limit of the D1-D5 string theory which is dual to AdS3 at strong coupling. In this context, we show how certain kinds of entwinement measure the lengths, in units of the AdS scale, of non-minimal geodesics present in certain excited states of the system which are gravitationally described as conical defects and the M = 0 BTZ black hole. The possible types of entwinement that can be computed define a very large new class of quantities characterizing the fine structure of quantum wavefunctions.

  3. Supervised Discrete Hashing With Relaxation.

    PubMed

    Gui, Jie; Liu, Tongliang; Sun, Zhenan; Tao, Dacheng; Tan, Tieniu

    2016-12-29

    Data-dependent hashing has recently attracted attention due to being able to support efficient retrieval and storage of high-dimensional data, such as documents, images, and videos. In this paper, we propose a novel learning-based hashing method called ''supervised discrete hashing with relaxation'' (SDHR) based on ''supervised discrete hashing'' (SDH). SDH uses ordinary least squares regression and traditional zero-one matrix encoding of class label information as the regression target (code words), thus fixing the regression target. In SDHR, the regression target is instead optimized. The optimized regression target matrix satisfies a large margin constraint for correct classification of each example. Compared with SDH, which uses the traditional zero-one matrix, SDHR utilizes the learned regression target matrix and, therefore, more accurately measures the classification error of the regression model and is more flexible. As expected, SDHR generally outperforms SDH. Experimental results on two large-scale image data sets (CIFAR-10 and MNIST) and a large-scale and challenging face data set (FRGC) demonstrate the effectiveness and efficiency of SDHR.

  4. Discreteness effects in population dynamics

    NASA Astrophysics Data System (ADS)

    Guevara Hidalgo, Esteban; Lecomte, Vivien

    2016-05-01

    We analyse numerically the effects of small population size in the initial transient regime of a simple example population dynamics. These effects play an important role for the numerical determination of large deviation functions of additive observables for stochastic processes. A method commonly used in order to determine such functions is the so-called cloning algorithm which in its non-constant population version essentially reduces to the determination of the growth rate of a population, averaged over many realizations of the dynamics. However, the averaging of populations is highly dependent not only on the number of realizations of the population dynamics, and on the initial population size but also on the cut-off time (or population) considered to stop their numerical evolution. This may result in an over-influence of discreteness effects at initial times, caused by small population size. We overcome these effects by introducing a (realization-dependent) time delay in the evolution of populations, additional to the discarding of the initial transient regime of the population growth where these discreteness effects are strong. We show that the improvement in the estimation of the large deviation function comes precisely from these two main contributions.

  5. Stabilizing a graphene platform toward discrete components

    NASA Astrophysics Data System (ADS)

    Mzali, Sana; Montanaro, Alberto; Xavier, Stéphane; Servet, Bernard; Mazellier, Jean-Paul; Bezencenet, Odile; Legagneux, Pierre; Piquemal-Banci, Maëlis; Galceran, Regina; Dlubak, Bruno; Seneor, Pierre; Martin, Marie-Blandine; Hofmann, Stephan; Robertson, John; Cojocaru, Costel-Sorin; Centeno, Alba; Zurutuza, Amaia

    2016-12-01

    We report on statistical analysis and consistency of electrical performances of devices based on a large scale passivated graphene platform. More than 500 graphene field effect transistors (GFETs) based on graphene grown by chemical vapor deposition and transferred on 4 in. SiO2/Si substrates were fabricated and tested. We characterized the potential of a two-step encapsulation process including an Al2O3 protection layer to avoid graphene contamination during the lithographic process followed by a final Al2O3 passivation layer subsequent to the GFET fabrication. Devices were investigated for occurrence and reproducibility of conductance minimum related to the Dirac point. While no conductance minimum was observed in unpassivated devices, 75% of the passivated transistors exhibited a clear conductance minimum and low hysteresis. The maximum of the device number distribution corresponds to a residual doping below 5 × 1011 cm-2 (0.023 V/nm). This yield shows that GFETs integrating low-doped graphene and exhibiting small hysteresis in the transfer characteristics can be envisaged for discrete components, with even further potential for low power driven electronics.

  6. Discrete Element Modeling for Mobility and Excavation

    NASA Astrophysics Data System (ADS)

    Knuth, M. A.; Hopkins, M. A.

    2011-12-01

    The planning and completion of mobility and excavation efforts on the moon requires a thorough understanding of the planetary regolith. In this work, a discrete element method (DEM) model is created to replicate those activities in the laboratory and for planning mission activities in the future. The crux of this work is developing a particle bed that best replicates the regolith tool/wheel interaction seen in the laboratory. To do this, a DEM geotechnical triaxial strength cell was created allowing for comparison of laboratory JSC-1a triaxial tests to DEM simulated soils. This model relies on a triangular lattice membrane covered triaxial cell for determining the macroscopic properties of the modeled granular material as well as a fast and efficient contact detection algorithm for a variety of grain shapes. Multiple grain shapes with increasing complexity (ellipsoid, poly-ellipsoid and polyhedra) have been developed and tested. This comparison gives us a basis to begin scaling DEM grain size and shape to practical values for mobility and excavation modeling. Next steps include development of a DEM scoop for percussive excavation testing as well as continued analysis of rover wheel interactions using a wide assortment of grain shape and size distributions.

  7. Discrete-time filtering of linear continuous-time processes

    NASA Astrophysics Data System (ADS)

    Shats, Samuel

    1989-06-01

    Continuous-time measurements are prefiltered before sampling, to remove additive white noise. The discrete-time optimal filter comprises a digital algorithm which is applied to the prefiltered, sampled measurements; the algorithm is based on the discrete-time equivalent model of the overall system. For the case of an integrate-and-dump analog prefilter, a discrete-time equivalent model was developed and the corresponding optimal filter was found for the general case, where the continuous-time measurement and process noise signals are correlated. A commonly used approximate discrete-time model was analyzed by defining and evaluating the true-error-covariance matrix of the estimate, and comparing it with the supposed error covariance matrix. It was shown that there is a class of unstable processes for which the former error covariance matrix attains unbounded norm, in spite of the continuing bounded nature of the other error covariance matrix. The main part of the thesis concerns the problem of finding an optimal prefilter. The steps of obtaining the optimal prefilter comprise: deriving a discrete-time equivalent-model of the overall system; finding the equation which is satisfied by the error covariance matrix; deriving the expressions which are satisfied by the first coefficients of the Maclaurin expansions of the error covariance matrix in the small parameter T; and obtaining the optimal prefilter by matrix optimization. The results obtained indicate that the optimal prefilter may be implemented through systems of different orders; the minimum order required is discussed, which is of great practical importance as the simplest possible prefilter. In discussion of the problem of discrete-time quadratic regulation of linear continuous time processes, the case of practical interest, where a zero-order hold is part of the digital-to-analog converter, is considered. It is shown that the duality between the regulation and filtering problems is not conserved after

  8. Golgi-Cox Staining Step by Step

    PubMed Central

    Zaqout, Sami; Kaindl, Angela M.

    2016-01-01

    Golgi staining remains a key method to study neuronal morphology in vivo. Since most protocols delineating modifications of the original staining method lack details on critical steps, establishing this method in a laboratory can be time-consuming and frustrating. Here, we describe the Golgi-Cox staining in such detail that should turn the staining into an easily feasible method for all scientists working in the neuroscience field. PMID:27065817

  9. Entropy is a consequence of a discrete time

    NASA Astrophysics Data System (ADS)

    Riek, Roland

    2015-07-01

    While the basic microscopic physical laws are time reversible, the arrow of time and time irreversibility appears only at the macroscopic physical laws by the second law of thermodynamics with its entropy term S. It is the attempt of the present work to bridge the microscopic physical world with its macroscopic one with an alternative approach than the statistical mechanics theory of Gibbs and Boltzmann. For simplicity a “classical”, single particle in a one dimensional space is selected. In addition, it is assumed that time is discrete with constant step size. As a consequence time irreversibility at the microscopic level is obtained if the present force is of complex nature (F(r) ≠ const). In order to compare this discrete time irreversible mechanics with its classical Newton analog, time reversibility is reintroduced by scaling the time steps for any given time step n by the variable sn leading to the Nosé-Hoover Lagrangian comprising a term NdfkB T In sn (kB the Boltzmann constant, T the temperature, and Ndf the number of degrees of freedom) which is defined as the microscopic entropy Sn at time point n multiplied by T. Upon ensemble averaging of the microscopic entropy in a many particles system in thermodynamic equilibrium it approximates its macroscopic counterpart known from statistical mechanics. The presented derivation with the resulting analogy between the ensemble averaged microscopic entropy and its statistical mechanics analog suggests that the entropy term itself has its root not in statistical mechanics but rather in the discreteness of time.

  10. Ideal shrinking and expansion of discrete sequences

    NASA Technical Reports Server (NTRS)

    Watson, Andrew B.

    1986-01-01

    Ideal methods are described for shrinking or expanding a discrete sequence, image, or image sequence. The methods are ideal in the sense that they preserve the frequency spectrum of the input up to the Nyquist limit of the input or output, whichever is smaller. Fast implementations that make use of the discrete Fourier transform or the discrete Hartley transform are described. The techniques lead to a new multiresolution image pyramid.

  11. Discrete modelling of drapery systems

    NASA Astrophysics Data System (ADS)

    Thoeni, Klaus; Giacomini, Anna

    2016-04-01

    Drapery systems are an efficient and cost-effective measure in preventing and controlling rockfall hazards on rock slopes. The simplest form consists of a row of ground anchors along the top of the slope connected to a horizontal support cable from which a wire mesh is suspended down the face of the slope. Such systems are generally referred to as simple or unsecured draperies (Badger and Duffy 2012). Variations such as secured draperies, where a pattern of ground anchors is incorporated within the field of the mesh, and hybrid systems, where the upper part of an unsecured drapery is elevated to intercept rockfalls originating upslope of the installation, are becoming more and more popular. This work presents a discrete element framework for simulation of unsecured drapery systems and its variations. The numerical model is based on the classical discrete element method (DEM) and implemented into the open-source framework YADE (Šmilauer et al., 2010). The model takes all relevant interactions between block, drapery and slope into account (Thoeni et al., 2014) and was calibrated and validated based on full-scale experiments (Giacomini et al., 2012).The block is modelled as a rigid clump made of spherical particles which allows any shape to be approximated. The drapery is represented by a set of spherical particle with remote interactions. The behaviour of the remote interactions is governed by the constitutive behaviour of the wire and generally corresponds to a piecewise linear stress-strain relation (Thoeni et al., 2013). The same concept is used to model wire ropes. The rock slope is represented by rigid triangular elements where material properties (e.g., normal coefficient of restitution, friction angle) are assigned to each triangle. The capabilities of the developed model to simulate drapery systems and estimate the residual hazard involved with such systems is shown. References Badger, T.C., Duffy, J.D. (2012) Drapery systems. In: Turner, A.K., Schuster R

  12. Structure of random discrete spacetime

    NASA Technical Reports Server (NTRS)

    Brightwell, Graham; Gregory, Ruth

    1991-01-01

    The usual picture of spacetime consists of a continuous manifold, together with a metric of Lorentzian signature which imposes a causal structure on the spacetime. A model, first suggested by Bombelli et al., is considered in which spacetime consists of a discrete set of points taken at random from a manifold, with only the causal structure on this set remaining. This structure constitutes a partially ordered set (or poset). Working from the poset alone, it is shown how to construct a metric on the space which closely approximates the metric on the original spacetime manifold, how to define the effective dimension of the spacetime, and how such quantities may depend on the scale of measurement. Possible desirable features of the model are discussed.

  13. Discrete breathers in hydrogenated graphene

    NASA Astrophysics Data System (ADS)

    Liu, Bo; Baimova, Julia A.; Dmitriev, Sergey V.; Wang, Xu; Zhu, Hongwei; Zhou, Kun

    2013-07-01

    Discrete breathers (DBs) in graphane (fully hydrogenated graphene) are investigated using molecular dynamics simulations. It is found that the DB can be excited by applying an out-of-plane displacement on a single hydrogen atom of graphane. The vibration frequency of the DB lies either within the gap of the phonon spectrum of graphane or beyond its upper spectrum bound. Both soft and hard types of anharmonicity of the DB, which have not been found in the same system, are observed in graphane. The study shows that the DB is robust and its lifetime is affected by various factors including its anharmonicity type, its amplitude and frequency, and the force on the hydrogen atom that forms it, whose competition results in a complex mechanism for the lifetime determination. The investigation of the maximum kinetic energy of DBs reveals that they may function to activate or accelerate dehydrogenation of hydrogenated graphene at high temperatures.

  14. Structure of random discrete spacetime

    NASA Technical Reports Server (NTRS)

    Brightwell, Graham; Gregory, Ruth

    1991-01-01

    The usual picture of spacetime consists of a continuous manifold, together with a metric of Lorentzian signature which imposes a causal structure on the spacetime. A model, first suggested by Bombelli et al., is considered in which spacetime consists of a discrete set of points taken at random from a manifold, with only the causal structure on this set remaining. This structure constitutes a partially ordered set (or poset). Working from the poset alone, it is shown how to construct a metric on the space which closely approximates the metric on the original spacetime manifold, how to define the effective dimension of the spacetime, and how such quantities may depend on the scale of measurement. Possible desirable features of the model are discussed.

  15. Scalar discrete nonlinear multipoint boundary value problems

    NASA Astrophysics Data System (ADS)

    Rodriguez, Jesus; Taylor, Padraic

    2007-06-01

    In this paper we provide sufficient conditions for the existence of solutions to scalar discrete nonlinear multipoint boundary value problems. By allowing more general boundary conditions and by imposing less restrictions on the nonlinearities, we obtain results that extend previous work in the area of discrete boundary value problems [Debra L. Etheridge, Jesus Rodriguez, Periodic solutions of nonlinear discrete-time systems, Appl. Anal. 62 (1996) 119-137; Debra L. Etheridge, Jesus Rodriguez, Scalar discrete nonlinear two-point boundary value problems, J. Difference Equ. Appl. 4 (1998) 127-144].

  16. A discrete event method for wave simulation

    SciTech Connect

    Nutaro, James J

    2006-01-01

    This article describes a discrete event interpretation of the finite difference time domain (FDTD) and digital wave guide network (DWN) wave simulation schemes. The discrete event method is formalized using the discrete event system specification (DEVS). The scheme is shown to have errors that are proportional to the resolution of the spatial grid. A numerical example demonstrates the relative efficiency of the scheme with respect to FDTD and DWN schemes. The potential for the discrete event scheme to reduce numerical dispersion and attenuation errors is discussed.

  17. Discrete gauge symmetry in continuum theories

    SciTech Connect

    Krauss, L.M.; Wilczek, F.

    1989-03-13

    We point out that local symmetries can masquerade as discrete global symmetries to an observer equipped with only low-energy probes. The existence of the underlying local gauge invariance can, however, result in observable Aharonov-Bohm-type effects. Black holes can therefore carry discrete gauge charges: a form of nonclassical ''hair.'' Neither black-hole evaporation, wormholes, nor anything else can violate discrete gauge symmetries. In supersymmetric unified theories such discrete symmetries can forbid proton-decay amplitudes that might otherwise be catastrophic.

  18. Control of discrete event systems modeled as hierarchical state machines

    NASA Technical Reports Server (NTRS)

    Brave, Y.; Heymann, M.

    1991-01-01

    The authors examine a class of discrete event systems (DESs) modeled as asynchronous hierarchical state machines (AHSMs). For this class of DESs, they provide an efficient method for testing reachability, which is an essential step in many control synthesis procedures. This method utilizes the asynchronous nature and hierarchical structure of AHSMs, thereby illustrating the advantage of the AHSM representation as compared with its equivalent (flat) state machine representation. An application of the method is presented where an online minimally restrictive solution is proposed for the problem of maintaining a controlled AHSM within prescribed legal bounds.

  19. A discourse on sensitivity analysis for discretely-modeled structures

    NASA Technical Reports Server (NTRS)

    Adelman, Howard M.; Haftka, Raphael T.

    1991-01-01

    A descriptive review is presented of the most recent methods for performing sensitivity analysis of the structural behavior of discretely-modeled systems. The methods are generally but not exclusively aimed at finite element modeled structures. Topics included are: selections of finite difference step sizes; special consideration for finite difference sensitivity of iteratively-solved response problems; first and second derivatives of static structural response; sensitivity of stresses; nonlinear static response sensitivity; eigenvalue and eigenvector sensitivities for both distinct and repeated eigenvalues; and sensitivity of transient response for both linear and nonlinear structural response.

  20. Information on stepping motors

    NASA Astrophysics Data System (ADS)

    Fongarland, G.

    1982-04-01

    The principles of the stepping motors which are often used in servomechanisms are reviewed. Variable reluctance as well as permanent magnet stepping motors are considered. Their operation is explained which includes permanent rotation, starting, stopping, and resonance effects. Several application examples, drawn from problems in automation, are outlined.

  1. STEP Experiment Requirements

    NASA Technical Reports Server (NTRS)

    Brumfield, M. L. (Compiler)

    1984-01-01

    A plan to develop a space technology experiments platform (STEP) was examined. NASA Langley Research Center held a STEP Experiment Requirements Workshop on June 29 and 30 and July 1, 1983, at which experiment proposers were invited to present more detailed information on their experiment concept and requirements. A feasibility and preliminary definition study was conducted and the preliminary definition of STEP capabilities and experiment concepts and expected requirements for support services are presented. The preliminary definition of STEP capabilities based on detailed review of potential experiment requirements is investigated. Topics discussed include: Shuttle on-orbit dynamics; effects of the space environment on damping materials; erectable beam experiment; technology for development of very large solar array deployers; thermal energy management process experiment; photovoltaic concentrater pointing dynamics and plasma interactions; vibration isolation technology; flight tests of a synthetic aperture radar antenna with use of STEP.

  2. Single step optimization of manipulator maneuvers with variable structure control

    NASA Technical Reports Server (NTRS)

    Chen, N.; Dwyer, T. A. W., III

    1987-01-01

    One step ahead optimization has been recently proposed for spacecraft attitude maneuvers as well as for robot manipulator maneuvers. Such a technique yields a discrete time control algorithm implementable as a sequence of state-dependent, quadratic programming problems for acceleration optimization. Its sensitivity to model accuracy, for the required inversion of the system dynamics, is shown in this paper to be alleviated by a fast variable structure control correction, acting between the sampling intervals of the slow one step ahead discrete time acceleration command generation algorithm. The slow and fast looping concept chosen follows that recently proposed for optimal aiming strategies with variable structure control. Accelerations required by the VSC correction are reserved during the slow one step ahead command generation so that the ability to overshoot the sliding surface is guaranteed.

  3. A first passage time distribution for a discrete version of the Ornstein Uhlenbeck process

    NASA Astrophysics Data System (ADS)

    Larralde, Hernán

    2004-03-01

    The probability of the first entrance to the negative semi-axis for a one-dimensional discrete Ornstein-Uhlenbeck (O-U) process is studied in this work. The discrete O-U process is a simple generalization of the random walk and many of its statistics may be calculated using essentially the same formalism. In particular, the case in which Sparre-Andersen's theorem applies for normal random walks is considered, and it is shown that the universal features of the first passage probability do not extend to the discrete O-U process. Finally, an explicit expression for the generating function of the probability of first entrance to the negative real axis at step n is calculated and analysed for a particular choice of the step distribution.

  4. On discrete symmetries for a whole Abelian model

    SciTech Connect

    Chauca, J.; Doria, R.

    2012-09-24

    Considering the whole concept applied to gauge theory a nonlinear abelian model is derived. A next step is to understand on the model properties. At this work, it will be devoted to discrete symmetries. For this, we will work based in two fields reference systems. This whole gauge symmetry allows to be analyzed through different sets which are the constructor basis {l_brace}D{sub {mu}},X{sup i}{sub {mu}}{r_brace} and the physical basis {l_brace}G{sub {mu}I}{r_brace}. Taking as fields reference system the diagonalized spin-1 sector, P, C, T and PCT symmetries are analyzed. They show that under this systemic model there are conservation laws driven for the parts and for the whole. It develops the meaning of whole-parity, field-parity and so on. However it is the whole symmetry that rules. This means that usually forbidden particles as pseudovector photons can be introduced through such whole abelian system. As result, one notices that the fields whole {l_brace}G{sub {mu}I}{r_brace} manifest a quanta diversity. It involves particles with different spins, masses and discrete quantum numbers under a same gauge symmetry. It says that without violating PCT symmetry different possibilities on discrete symmetries can be accommodated.

  5. Dust grain coagulation modelling : From discrete to continuous

    NASA Astrophysics Data System (ADS)

    Paruta, P.; Hendrix, T.; Keppens, R.

    2016-07-01

    In molecular clouds, stars are formed from a mixture of gas, plasma and dust particles. The dynamics of this formation is still actively investigated and a study of dust coagulation can help to shed light on this process. Starting from a pre-existing discrete coagulation model, this work aims to mathematically explore its properties and its suitability for numerical validation. The crucial step is in our reinterpretation from its original discrete to a well-defined continuous form, which results in the well-known Smoluchowski coagulation equation. This opens up the possibility of exploiting previous results in order to prove the existence and uniqueness of a mass conserving solution for the evolution of dust grain size distribution. Ultimately, to allow for a more flexible numerical implementation, the problem is rewritten as a non-linear hyperbolic integro-differential equation and solved using a finite volume discretisation. It is demonstrated that there is an exact numerical agreement with the initial discrete model, with improved accuracy. This is of interest for further work on dynamically coupled gas with dust simulations.

  6. Interface discrete light bullets in waveguide arrays.

    PubMed

    Mihalache, Dumitru; Mazilu, Dumitru; Lederer, Falk; Kivshar, Yuri S

    2007-08-01

    We analyze spatiotemporal light localization at the interface separating two different periodic photonic lattices. We demonstrate the existence of a novel class of continuous-discrete spatiotemporal solitons propagating along the interface, including hybrid staggered-unstaggered discrete light bullets with tails belonging to spectral gaps of different types.

  7. Current Density and Continuity in Discretized Models

    ERIC Educational Resources Information Center

    Boykin, Timothy B.; Luisier, Mathieu; Klimeck, Gerhard

    2010-01-01

    Discrete approaches have long been used in numerical modelling of physical systems in both research and teaching. Discrete versions of the Schrodinger equation employing either one or several basis functions per mesh point are often used by senior undergraduates and beginning graduate students in computational physics projects. In studying…

  8. Conservative discretization of the Landau collision integral

    DOE PAGES

    Hirvijoki, E.; Adams, M. F.

    2017-03-28

    Here we describe a density, momentum-, and energy-conserving discretization of the nonlinear Landau collision integral. The method is suitable for both the finite-element and discontinuous Galerkin methods and does not require structured meshes. The conservation laws for the discretization are proven algebraically and demonstrated numerically for an axially symmetric nonlinear relaxation problem using a finite-element implementation.

  9. Current Density and Continuity in Discretized Models

    ERIC Educational Resources Information Center

    Boykin, Timothy B.; Luisier, Mathieu; Klimeck, Gerhard

    2010-01-01

    Discrete approaches have long been used in numerical modelling of physical systems in both research and teaching. Discrete versions of the Schrodinger equation employing either one or several basis functions per mesh point are often used by senior undergraduates and beginning graduate students in computational physics projects. In studying…

  10. Quantifying Discretization Effects on Brain Trauma Simulations

    DTIC Science & Technology

    2016-01-01

    analyzed in each case were the variations in stress magnitude, spatial distribution, and wave patterns that arise inside the brain. The effects of...ARL-CR-0792 ● JAN 2016 US Army Research Laboratory Quantifying Discretization Effects on Brain Trauma Simulations prepared by...originator. ARL-CR-0792● JAN 2016 US Army Research Laboratory Quantifying Discretization Effects on Brain Trauma Simulations

  11. The Role of Procedural Integrity: Using Self-Monitoring to Enhance Discrete Trial Instruction (DTI)

    ERIC Educational Resources Information Center

    Belfiore, Phillip J.; Fritts, Kevin M.; Herman, Brian C.

    2008-01-01

    The purpose of this study was to examine the effects of staff video self-monitoring (SM) training on the accuracy of delivering Discrete Trial Instruction (DTI) to students enrolled in a classroom for children with autism. Staff were trained to self-monitor a five-step DTI trial: (a) delivery of discriminative stimulus, (b) wait time for student…

  12. Component Evaluation of a Computer Based Format for Teaching Discrete Trial and Backward Chaining

    ERIC Educational Resources Information Center

    Nosik, Melissa R.; Williams, W. Larry

    2011-01-01

    The effectiveness of a multi-component computer based training package that consisted of competency based instructions, video modeling, and two forms of feedback was evaluated in terms of treatment integrity of two procedures across four staff. Treatment integrity in completing critical steps of discrete-trial and backward chaining procedures were…

  13. The Next Giant Step

    NASA Image and Video Library

    Artist Robert McCall painted "The Next Giant Step" in 1979 to commemorate the heroism and courage of spaceflight pioneers. Located in the lobby of Johnson's building 2, the mural depicts America's ...

  14. The California "Step System."

    ERIC Educational Resources Information Center

    Manaster, Alfred

    1985-01-01

    The University of California's "step" system for appointment and advancement of faculty and the salary scale attached to it are outlined, and some criteria and procedures used in making academic personnel decisions are reviewed. (MSE)

  15. Middle School STEP Act

    THOMAS, 113th Congress

    Sen. Kaine, Tim [D-VA

    2014-09-10

    Senate - 09/10/2014 Read twice and referred to the Committee on Health, Education, Labor, and Pensions. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  16. CEIP Next Steps

    EPA Pesticide Factsheets

    The Clean Energy Incentive Program (CEIP) next steps document details the EPA’s outreach strategy for stakeholder input on the design and implementation of the CEIP. Additionally, this document lists provisions on the CEIP where stakeholder input is sought

  17. Generalized exponential function and discrete growth models

    NASA Astrophysics Data System (ADS)

    Souto Martinez, Alexandre; Silva González, Rodrigo; Lauri Espíndola, Aquino

    2009-07-01

    Here we show that a particular one-parameter generalization of the exponential function is suitable to unify most of the popular one-species discrete population dynamic models into a simple formula. A physical interpretation is given to this new introduced parameter in the context of the continuous Richards model, which remains valid for the discrete case. From the discretization of the continuous Richards’ model (generalization of the Gompertz and Verhulst models), one obtains a generalized logistic map and we briefly study its properties. Notice, however that the physical interpretation for the introduced parameter persists valid for the discrete case. Next, we generalize the (scramble competition) θ-Ricker discrete model and analytically calculate the fixed points as well as their stabilities. In contrast to previous generalizations, from the generalized θ-Ricker model one is able to retrieve either scramble or contest models.

  18. Active control of turbomachine discrete tones

    NASA Astrophysics Data System (ADS)

    Fleeter, Sanford

    This paper was directed at active control of discrete frequency noise generated by subsonic blade rows through cancellation of the blade row interaction generated propagating acoustic waves. First discrete frequency noise generated by a rotor and stator in a duct was analyzed to determine the propagating acoustic pressure waves. Then a mathematical model was developed to analyze and predict the active control of discrete frequency noise generated by subsonic blade rows through cancellation of the propagating acoustic waves, accomplished by utilizing oscillating airfoil surfaces to generate additional control propagating pressure waves. These control waves interact with the propagating acoustic waves, thereby, in principle, canceling the acoustic waves and thus, the far field discrete frequency tones. This model was then applied to a fan exit guide vane to investigate active airfoil surface techniques for control of the propagating acoustic waves, and thus the far field discrete frequency tones, generated by blade row interactions.

  19. Discrete breathers in graphane: Effect of temperature

    NASA Astrophysics Data System (ADS)

    Baimova, J. A.; Murzaev, R. T.; Lobzenko, I. P.; Dmitriev, S. V.; Zhou, Kun

    2016-05-01

    The discrete breathers in graphane in thermodynamic equilibrium in the temperature range 50-600 K are studied by molecular dynamics simulation. A discrete breather is a hydrogen atom vibrating along the normal to a sheet of graphane at a high amplitude. As was found earlier, the lifetime of a discrete breather at zero temperature corresponds to several tens of thousands of vibrations. The effect of temperature on the decay time of discrete breathers and the probability of their detachment from a sheet of graphane are studied in this work. It is shown that closely spaced breathers can exchange energy with each other at zero temperature. The data obtained suggest that thermally activated discrete breathers can be involved in the dehydrogenation of graphane, which is important for hydrogen energetics.

  20. Discrete breathers in graphane: Effect of temperature

    SciTech Connect

    Baimova, J. A.; Murzaev, R. T.; Lobzenko, I. P.; Dmitriev, S. V.; Zhou, Kun

    2016-05-15

    The discrete breathers in graphane in thermodynamic equilibrium in the temperature range 50–600 K are studied by molecular dynamics simulation. A discrete breather is a hydrogen atom vibrating along the normal to a sheet of graphane at a high amplitude. As was found earlier, the lifetime of a discrete breather at zero temperature corresponds to several tens of thousands of vibrations. The effect of temperature on the decay time of discrete breathers and the probability of their detachment from a sheet of graphane are studied in this work. It is shown that closely spaced breathers can exchange energy with each other at zero temperature. The data obtained suggest that thermally activated discrete breathers can be involved in the dehydrogenation of graphane, which is important for hydrogen energetics.

  1. Discrete multiscale wavelet shrinkage and integrodifferential equations

    NASA Astrophysics Data System (ADS)

    Didas, S.; Steidl, G.; Weickert, J.

    2008-04-01

    We investigate the relation between discrete wavelet shrinkage and integrodifferential equations in the context of simplification and denoising of one-dimensional signals. In the continuous setting, strong connections between these two approaches were discovered in 6 (see references). The key observation is that the wavelet transform can be understood as derivative operator after the convolution with a smoothing kernel. In this paper, we extend these ideas to the practically relevant discrete setting with both orthogonal and biorthogonal wavelets. In the discrete case, the behaviour of the smoothing kernels for different scales requires additional investigation. The results of discrete multiscale wavelet shrinkage and related discrete versions of integrodifferential equations are compared with respect to their denoising quality by numerical experiments.

  2. Laplacians on discrete and quantum geometries

    NASA Astrophysics Data System (ADS)

    Calcagni, Gianluca; Oriti, Daniele; Thürigen, Johannes

    2013-06-01

    We extend discrete calculus for arbitrary (p-form) fields on embedded lattices to abstract discrete geometries based on combinatorial complexes. We then provide a general definition of discrete Laplacian using both the primal cellular complex and its combinatorial dual. The precise implementation of geometric volume factors is not unique and, comparing the definition with a circumcentric and a barycentric dual, we argue that the latter is, in general, more appropriate because it induces a Laplacian with more desirable properties. We give the expression of the discrete Laplacian in several different sets of geometric variables, suitable for computations in different quantum gravity formalisms. Furthermore, we investigate the possibility of transforming from position to momentum space for scalar fields, thus setting the stage for the calculation of heat kernel and spectral dimension in discrete quantum geometries.

  3. Viscoelasticity of stepped interfaces

    NASA Astrophysics Data System (ADS)

    Skirlo, S. A.; Demkowicz, M. J.

    2013-10-01

    Using molecular dynamics modeling, we show that interfaces in sputter deposited Cu-Nb superlattices exhibit time-dependent elasticity, i.e., viscoelasticity, under shear loading. In the high temperature and small strain rate limit, the interfacial shear modulus approaches a value proportional to the density of steps in the interface. It may therefore be possible to tailor the low-frequency shear moduli of interfaces by controlling their step densities.

  4. On the consistency between nearest-neighbor peridynamic discretizations and discretized classical elasticity models

    SciTech Connect

    Seleson, Pablo; Du, Qiang; Parks, Michael L.

    2016-08-16

    The peridynamic theory of solid mechanics is a nonlocal reformulation of the classical continuum mechanics theory. At the continuum level, it has been demonstrated that classical (local) elasticity is a special case of peridynamics. Such a connection between these theories has not been extensively explored at the discrete level. This paper investigates the consistency between nearest-neighbor discretizations of linear elastic peridynamic models and finite difference discretizations of the Navier–Cauchy equation of classical elasticity. While nearest-neighbor discretizations in peridynamics have been numerically observed to present grid-dependent crack paths or spurious microcracks, this paper focuses on a different, analytical aspect of such discretizations. We demonstrate that, even in the absence of cracks, such discretizations may be problematic unless a proper selection of weights is used. Specifically, we demonstrate that using the standard meshfree approach in peridynamics, nearest-neighbor discretizations do not reduce, in general, to discretizations of corresponding classical models. We study nodal-based quadratures for the discretization of peridynamic models, and we derive quadrature weights that result in consistency between nearest-neighbor discretizations of peridynamic models and discretized classical models. The quadrature weights that lead to such consistency are, however, model-/discretization-dependent. We motivate the choice of those quadrature weights through a quadratic approximation of displacement fields. The stability of nearest-neighbor peridynamic schemes is demonstrated through a Fourier mode analysis. Finally, an approach based on a normalization of peridynamic constitutive constants at the discrete level is explored. This approach results in the desired consistency for one-dimensional models, but does not work in higher dimensions. The results of the work presented in this paper suggest that even though nearest

  5. On the consistency between nearest-neighbor peridynamic discretizations and discretized classical elasticity models

    SciTech Connect

    Seleson, Pablo; Du, Qiang; Parks, Michael L.

    2016-08-16

    The peridynamic theory of solid mechanics is a nonlocal reformulation of the classical continuum mechanics theory. At the continuum level, it has been demonstrated that classical (local) elasticity is a special case of peridynamics. Such a connection between these theories has not been extensively explored at the discrete level. This paper investigates the consistency between nearest-neighbor discretizations of linear elastic peridynamic models and finite difference discretizations of the Navier–Cauchy equation of classical elasticity. While nearest-neighbor discretizations in peridynamics have been numerically observed to present grid-dependent crack paths or spurious microcracks, this paper focuses on a different, analytical aspect of such discretizations. We demonstrate that, even in the absence of cracks, such discretizations may be problematic unless a proper selection of weights is used. Specifically, we demonstrate that using the standard meshfree approach in peridynamics, nearest-neighbor discretizations do not reduce, in general, to discretizations of corresponding classical models. We study nodal-based quadratures for the discretization of peridynamic models, and we derive quadrature weights that result in consistency between nearest-neighbor discretizations of peridynamic models and discretized classical models. The quadrature weights that lead to such consistency are, however, model-/discretization-dependent. We motivate the choice of those quadrature weights through a quadratic approximation of displacement fields. The stability of nearest-neighbor peridynamic schemes is demonstrated through a Fourier mode analysis. Finally, an approach based on a normalization of peridynamic constitutive constants at the discrete level is explored. This approach results in the desired consistency for one-dimensional models, but does not work in higher dimensions. The results of the work presented in this paper suggest that even though nearest

  6. MULTISCALE DISCRETIZATION OF SHAPE CONTOURS

    SciTech Connect

    Prasad, L.; Rao, R.

    2000-09-01

    We present an efficient multi-scale scheme to adaptively approximate the continuous (or densely sampled) contour of a planar shape at varying resolutions. The notion of shape is intimately related to the notion of contour, and the efficient representation of the contour of a shape is vital to a computational understanding of the shape. Any polygonal approximation of a planar smooth curve is equivalent to a piecewise constant approximation of the parameterized X and Y coordinate functions of a discrete point set obtained by densely sampling the curve. Using the Haar wavelet transform for the piecewise approximation yields a hierarchical scheme in which the size of the approximating point set is traded off against the morphological accuracy of the approximation. Our algorithm compresses the representation of the initial shape contour to a sparse sequence of points in the plane defining the vertices of the shape's polygonal approximation. Furthermore, it is possible to control the overall resolution of the approximation by a single, scale-independent parameter.

  7. Seipin Is a Discrete Homooligomer†

    PubMed Central

    Binns, Derk; Lee, SungKyung; Hilton, Christopher L.; Jiang, Qiu-Xing; Goodman, Joel M.

    2011-01-01

    Seipin is a transmembrane protein that resides in the endoplasmic reticulum and concentrates at junctions between the ER and cytosolic lipid droplets. Mutations in the human seipin gene, including the missense mutation A212P, lead to congenital generalized lipodystrophy (CGL), characterized by the lack of normal adipose tissue and accumulation of fat in liver and muscles. In both yeast and CGL patient fibroblasts, seipin is required for normal lipid droplet morphology; in its absence droplets appear to bud abnormally from the ER. Here we report the first purification and physical characterization of seipin. Yeast seipin is in a large discrete protein complex. Affinity purification demonstrated that seipin is the main if not exclusive protein in the complex. Detergent sucrose gradients in H2O, and D2O and gel filtration were used to determine the size of the seipin complex and account for detergent binding. Both seipin-myc13 (seipin fused to 13 tandem copies of the myc epitope) expressed from the endogenous promoter and overexpressed seipin-mCherry form ~500 kDa proteins consisting of about 9 copies of seipin. The yeast orthologue of the human A212P allele forms only smaller complexes and is unstable; we hypothesize that this accounts for its null phenotype in humans. Seipin appears as a toroid by negative staining electron microscopy. We speculate that seipin plays at least a structural role in organizing droplets or in communication between droplets and ER. PMID:21062080

  8. Novel discretization schemes for the numerical simulation of membrane dynamics

    NASA Astrophysics Data System (ADS)

    Kolsti, Kyle F.

    Motivated by the demands of simulating flapping wings of Micro Air Vehicles, novel numerical methods were developed and evaluated for the dynamic simulation of membranes. For linear membranes, a mixed-form time-continuous Galerkin method was employed using trilinear space-time elements. Rather than time-marching, the entire space-time domain was discretized and solved simultaneously. Second-order rates of convergence in both space and time were observed in numerical studies. Slight high-frequency noise was filtered during post-processing. For geometrically nonlinear membranes, the model incorporated two new schemes that were independently developed and evaluated. Time marching was performed using quintic Hermite polynomials uniquely determined by end-point jerk constraints. The single-step, implicit scheme was significantly more accurate than the most common Newmark schemes. For a simple harmonic oscillator, the scheme was found to be symplectic, frequency-preserving, and conditionally stable. Time step size was limited by accuracy requirements rather than stability. The spatial discretization scheme employed a staggered grid, grouping of nonlinear terms, and polygon shape functions in a strong-form point collocation formulation. The observed rate of convergence was two for both displacement and strain. Validation against existing experimental data showed the method to be accurate until hyperelastic effects dominate.

  9. Low sidelobe nonlinear stepped-frequency waveforms

    NASA Astrophysics Data System (ADS)

    Chebanov, Dmitry

    2008-04-01

    Frequency stepping is one of the known techniques employed by modern radars to attain high range resolution. One of the main advantages of this approach is that it allows to achieve wideband pulse compression through narrowband processing. It is also known that the traditional linear stepped-frequency waveform suffers from relatively high range sidelobes and grating lobes that appear due to periodicities in the Discrete Fourier Transform (DFT). An amplitude weighting (applied prior to the DFT) is typically used to reduce the near-in sidelobes. This results in undesirable losses in sensitivity. In this paper, we propose a new approach that may be used to derive families of nonlinear stepped-frequency waveforms that would have desired characteristics such as suppressed grating lobes and built-in low range sidelobes. Our approach is based on new analytical properties of stepped-frequency waveforms presented in the paper. We give examples of nonlinear waveforms generated by this approach and show that they exhibit improved performance when compared with traditional waveforms.

  10. Compatible Spatial Discretizations for Partial Differential Equations

    SciTech Connect

    Arnold, Douglas, N, ed.

    2004-11-25

    From May 11--15, 2004, the Institute for Mathematics and its Applications held a hot topics workshop on Compatible Spatial Discretizations for Partial Differential Equations. The numerical solution of partial differential equations (PDE) is a fundamental task in science and engineering. The goal of the workshop was to bring together a spectrum of scientists at the forefront of the research in the numerical solution of PDEs to discuss compatible spatial discretizations. We define compatible spatial discretizations as those that inherit or mimic fundamental properties of the PDE such as topology, conservation, symmetries, and positivity structures and maximum principles. A wide variety of discretization methods applied across a wide range of scientific and engineering applications have been designed to or found to inherit or mimic intrinsic spatial structure and reproduce fundamental properties of the solution of the continuous PDE model at the finite dimensional level. A profusion of such methods and concepts relevant to understanding them have been developed and explored: mixed finite element methods, mimetic finite differences, support operator methods, control volume methods, discrete differential forms, Whitney forms, conservative differencing, discrete Hodge operators, discrete Helmholtz decomposition, finite integration techniques, staggered grid and dual grid methods, etc. This workshop seeks to foster communication among the diverse groups of researchers designing, applying, and studying such methods as well as researchers involved in practical solution of large scale problems that may benefit from advancements in such discretizations; to help elucidate the relations between the different methods and concepts; and to generally advance our understanding in the area of compatible spatial discretization methods for PDE. Particular points of emphasis included: + Identification of intrinsic properties of PDE models that are critical for the fidelity of numerical

  11. On the definition of discrete hydrodynamic variables.

    PubMed

    Español, Pep; Zúñiga, Ignacio

    2009-10-28

    The Green-Kubo formula for discrete hydrodynamic variables involves information about not only the fluid transport coefficients but also about discrete versions of the differential operators that govern the evolution of the discrete variables. This gives an intimate connection between discretization procedures in fluid dynamics and coarse-graining procedures used to obtain hydrodynamic behavior of molecular fluids. We observed that a natural definition of discrete hydrodynamic variables in terms of Voronoi cells leads to a Green-Kubo formula which is divergent, rendering the full coarse-graining strategy useless. In order to understand this subtle issue, in the present paper we consider the coarse graining of noninteracting Brownian particles. The discrete hydrodynamic variable for this problem is the number of particles within Voronoi cells. Thanks to the simplicity of the model we spot the origin of the singular behavior of the correlation functions. We offer an alternative definition, based on the concept of a Delaunay cell that behaves properly, suggesting the use of the Delaunay construction for the coarse graining of molecular fluids at the discrete hydrodynamic level.

  12. Discrete flavour symmetries from the Heisenberg group

    NASA Astrophysics Data System (ADS)

    Floratos, E. G.; Leontaris, G. K.

    2016-04-01

    Non-abelian discrete symmetries are of particular importance in model building. They are mainly invoked to explain the various fermion mass hierarchies and forbid dangerous superpotential terms. In string models they are usually associated to the geometry of the compactification manifold and more particularly to the magnetised branes in toroidal compactifications. Motivated by these facts, in this note we propose a unified framework to construct representations of finite discrete family groups based on the automorphisms of the discrete and finite Heisenberg group. We focus in particular, on the PSL2 (p) groups which contain the phenomenologically interesting cases.

  13. Discrete Elements Method of Neutral Particle Transport

    DTIC Science & Technology

    1983-10-01

    0 ,,S" . K A t - "’.* DISCRETE ELEMENTS METHOD NEUTRAL PARTICLE TRANSPORT ..- DISSERTATION AF. IT/DS/PH/83-5 Kirk A. Mathews LCDR USN D~ Approved for...DISCRETE ELEMENTS METHOD OF NEUTRAL PARTICLE TRANSPORT DISSERTATION Presented to the Faculty of the School of Engineering of the Air Force Institute...o .°. .• . r •.- - DS/PH/83-5 DISCRETE ELEMENTS METHOD OF NEUTRAL PARTICLE TRANSPORT 4 "by Kirk A. Mathews LCDR USN Approved: , Charles J

  14. Hairs of discrete symmetries and gravity

    NASA Astrophysics Data System (ADS)

    Choi, Kang Sin; Kim, Jihn E.; Kyae, Bumseok; Nam, Soonkeon

    2017-06-01

    Gauge symmetries are known to be respected by gravity because gauge charges carry flux lines, but global charges do not carry flux lines and are not conserved by gravitational interaction. For discrete symmetries, they are spontaneously broken in the Universe, forming domain walls. Since the realization of discrete symmetries in the Universe must involve the vacuum expectation values of Higgs fields, a string-like configuration (hair) at the intersection of domain walls in the Higgs vacua can be realized. Therefore, we argue that discrete charges are also respected by gravity.

  15. Stochastic Kuramoto oscillators with discrete phase states

    NASA Astrophysics Data System (ADS)

    Jörg, David J.

    2017-09-01

    We present a generalization of the Kuramoto phase oscillator model in which phases advance in discrete phase increments through Poisson processes, rendering both intrinsic oscillations and coupling inherently stochastic. We study the effects of phase discretization on the synchronization and precision properties of the coupled system both analytically and numerically. Remarkably, many key observables such as the steady-state synchrony and the quality of oscillations show distinct extrema while converging to the classical Kuramoto model in the limit of a continuous phase. The phase-discretized model provides a general framework for coupled oscillations in a Markov chain setting.

  16. Discrete symmetries and de Sitter spacetime

    SciTech Connect

    Cotăescu, Ion I. Pascu, Gabriel

    2014-11-24

    Aspects of the ambiguity in defining quantum modes on de Sitter spacetime using a commuting system composed only of differential operators are discussed. Discrete symmetries and their actions on the wavefunction in commonly used coordinate charts are reviewed. It is argued that the system of commuting operators can be supplemented by requiring the invariance of the wavefunction to combined discrete symmetries- a criterion which selects a single state out of the α-vacuum family. Two such members of this family are singled out by particular combined discrete symmetries- states between which exists a well-known thermality relation.

  17. Space-time adaptive solution of inverse problems with the discrete adjoint method

    NASA Astrophysics Data System (ADS)

    Alexe, Mihai; Sandu, Adrian

    2014-08-01

    This paper develops a framework for the construction and analysis of discrete adjoint sensitivities in the context of time dependent, adaptive grid, adaptive step models. Discrete adjoints are attractive in practice since they can be generated with low effort using automatic differentiation. However, this approach brings several important challenges. The space-time adjoint of the forward numerical scheme may be inconsistent with the continuous adjoint equations. A reduction in accuracy of the discrete adjoint sensitivities may appear due to the inter-grid transfer operators. Moreover, the optimization algorithm may need to accommodate state and gradient vectors whose dimensions change between iterations. This work shows that several of these potential issues can be avoided through a multi-level optimization strategy using discontinuous Galerkin (DG) hp-adaptive discretizations paired with Runge-Kutta (RK) time integration. We extend the concept of dual (adjoint) consistency to space-time RK-DG discretizations, which are then shown to be well suited for the adaptive solution of time-dependent inverse problems. Furthermore, we prove that DG mesh transfer operators on general meshes are also dual consistent. This allows the simultaneous derivation of the discrete adjoint for both the numerical solver and the mesh transfer logic with an automatic code generation mechanism such as algorithmic differentiation (AD), potentially speeding up development of large-scale simulation codes. The theoretical analysis is supported by numerical results reported for a two-dimensional non-stationary inverse problem.

  18. Dispersion reducing methods for edge discretizations of the electric vector wave equation

    NASA Astrophysics Data System (ADS)

    Bokil, V. A.; Gibson, N. L.; Gyrya, V.; McGregor, D. A.

    2015-04-01

    We present a novel strategy for minimizing the numerical dispersion error in edge discretizations of the time-domain electric vector wave equation on square meshes based on the mimetic finite difference (MFD) method. We compare this strategy, called M-adaptation, to two other discretizations, also based on square meshes. One is the lowest order Nédélec edge element discretization. The other is a modified quadrature approach (GY-adaptation) proposed by Guddati and Yue for the acoustic wave equation in two dimensions. All three discrete methods use the same edge-based degrees of freedom, while the temporal discretization is performed using the standard explicit Leapfrog scheme. To obtain efficient and explicit time stepping methods, the three schemes are further mass lumped. We perform a dispersion and stability analysis for the presented schemes and compare all three methods in terms of their stability regions and phase error. Our results indicate that the method produced by GY-adaptation and the Nédélec method are both second order accurate for numerical dispersion, but differ in the order of their numerical anisotropy (fourth order, versus second order, respectively). The result of M-adaptation is a discretization that is fourth order accurate for numerical dispersion as well as numerical anisotropy. Numerical simulations are provided that illustrate the theoretical results.

  19. The SMM Model as a Boundary Value Problem Using the Discrete Diffusion Equation

    NASA Technical Reports Server (NTRS)

    Campbell, Joel

    2007-01-01

    A generalized single step stepwise mutation model (SMM) is developed that takes into account an arbitrary initial state to a certain partial difference equation. This is solved in both the approximate continuum limit and the more exact discrete form. A time evolution model is developed for Y DNA or mtDNA that takes into account the reflective boundary modeling minimum microsatellite length and the original difference equation. A comparison is made between the more widely known continuum Gaussian model and a discrete model, which is based on modified Bessel functions of the first kind. A correction is made to the SMM model for the probability that two individuals are related that takes into account a reflecting boundary modeling minimum microsatellite length. This method is generalized to take into account the general n-step model and exact solutions are found. A new model is proposed for the step distribution.

  20. The SMM model as a boundary value problem using the discrete diffusion equation.

    PubMed

    Campbell, Joel

    2007-12-01

    A generalized single-step stepwise mutation model (SMM) is developed that takes into account an arbitrary initial state to a certain partial difference equation. This is solved in both the approximate continuum limit and the more exact discrete form. A time evolution model is developed for Y DNA or mtDNA that takes into account the reflective boundary modeling minimum microsatellite length and the original difference equation. A comparison is made between the more widely known continuum Gaussian model and a discrete model, which is based on modified Bessel functions of the first kind. A correction is made to the SMM model for the probability that two individuals are related that takes into account a reflecting boundary modeling minimum microsatellite length. This method is generalized to take into account the general n-step model and exact solutions are found. A new model is proposed for the step distribution.

  1. Mechanisms of polarized membrane trafficking in neurons – focusing in on endosomes

    PubMed Central

    Lasiecka, Zofia M.; Winckler, Bettina

    2011-01-01

    Neurons are polarized cells that have a complex and unique morphology: long processes (axons and dendrites) extending far from the cell body. In addition, the somatodendritic and axonal domains are further divided into specific subdomains, such as synapses (pre- and postsynaptic specializations), proximal and distal dendrites, axon initial segments, nodes of Ranvier, and axon growth cones. The striking asymmetry and complexity of neuronal cells is necessary for their function in receiving, processing and transferring electrical signals, with each domain playing a precise function in these processes. In order to establish and maintain distinct neuronal domains, mechanisms must exist for protein delivery to specific neuronal compartments, such that each compartment has the correct functional molecular composition. How polarized membrane domains are established and maintained is a long-standing question. Transmembrane proteins, such as receptors and adhesion molecules, can be transported to their proper membrane domains by several pathways. The biosynthetic secretory system delivers newly synthesized transmembrane proteins from the ER-Golgi via the trans-Golgi network (TGN) to the plasma membrane. In addition, the endosomal system is critically involved in many instances in ensuring proper (re)targeting of membrane components because it can internalize and degrade mislocalized proteins, or recycle proteins from one domain to another. The endosomal system is thus crucial for establishing and maintaining neuronal polarity. In this review, we focus mainly on the intracellular compartments that serve as sorting stations for polarized transport, with particular emphasis on the emerging roles of endosomes. PMID:21762782

  2. One-way membrane trafficking of SOS in receptor-triggered Ras activation

    PubMed Central

    Christensen, Sune M.; Tu, Hsiung-Lin; Jun, Jesse E.; Alvarez, Steven; Triplet, Meredith G.; Iwig, Jeffrey S.; Yadav, Kamlesh K.; Bar-Sagi, Dafna; Roose, Jeroen P.; Groves, Jay T.

    2016-01-01

    SOS is a key activator of the small GTPase Ras. In cells, SOS-Ras signaling is thought to be initiated predominantly by membrane-recruitment of SOS via the adaptor Grb2 and balanced by rapidly reversible Grb2:SOS binding kinetics. However, SOS has multiple protein and lipid interactions that provide linkage to the membrane. In reconstituted membrane experiments, these Grb2-independent interactions are sufficient to retain SOS on the membrane for many minutes, during which a single SOS molecule can processively activate thousands of Ras molecules. These observations raise questions concerning how receptors maintain control of SOS in cells and how membrane-recruited SOS is ultimately released. We addressed these questions in quantitative reconstituted SOS-deficient chicken B cell signaling systems combined with single molecule measurements in supported membranes. These studies reveal an essentially one-way trafficking process in which membrane-recruited SOS remains trapped on the membrane and continuously activates Ras until it is actively removed via endocytosis. PMID:27501536

  3. Regulation of the epithelial sodium channel (ENaC) by membrane trafficking

    PubMed Central

    Butterworth, Michael B.

    2010-01-01

    The epithelial Na+ channel (ENaC) is a major regulator of salt and water reabsorption in a number of epithelial tissues. Abnormalities in ENaC function have been directly linked to several human disease states including Liddle syndrome, psuedohypoaldosteronism and cystic fibrosis, and may be implicated in salt-sensitive hypertension. ENaC activity in epithelial cells is regulated both by open probability and channel number. This review focuses on the regulation of ENaC in the cells of the kidney cortical collecting duct by trafficking and recycling. The trafficking of ENaC is discussed in the broader context of epithelial cell vesicle trafficking. Well characterized pathways and protein interactions elucidated using epithelial model cells are discussed and the known overlap with ENaC regulation is highlighted. In following the life of ENaC in CCD epithelial cells the apical delivery, internalization, recycling and destruction of the channel will be discussed. While a number of pathways presented still need to be linked to ENaC regulation and many details of the regulation of ENaC trafficking remain to be elucidated, knowledge of these mechanisms may provide further insights into ENaC activity in normal and disease states. PMID:20347969

  4. Second messengers and membrane trafficking direct and organize growth cone steering

    PubMed Central

    Tojima, Takuro; Hines, Jacob H.; Henley, John R.; Kamiguchi, Hiroyuki

    2011-01-01

    Graded distributions of extracellular cues guide developing axons toward their targets. A network of second messengers, Ca2+ and cyclic nucleotides, shapes cue-derived information into either attractive or repulsive signals that steer growth cones bidirectionally. Emerging evidence suggests that such guidance signals create a localized imbalance between exocytosis and endocytosis, which in turn redirects membrane, adhesion and cytoskeletal components asymmetrically across the growth cone to bias the direction of axon extension. These recent advances allow us to propose a unifying model of how the growth cone translates shallow gradients of environmental information into polarized activity of the steering machinery for axon guidance. PMID:21386859

  5. Calcium-Modulating Cyclophilin Ligand Regulates Membrane Trafficking of Postsynaptic GABAA Receptors

    PubMed Central

    Yuan, Xu; Yao, Jun; Norris, David; Tran, David D.; Bram, Richard J.; Chen, Gong; Luscher, Bernhard

    2008-01-01

    Accumulation of GABAA receptors (GABAARs) at GABAergic synapses requires the cytoplasmic loop region and C-terminal transmembrane domain of the receptor γ2 subunit. We here report a novel interaction of γ2 with Calcium-Modulating cyclophilin Ligand (CAML), an integral membrane protein that regulates this mechanism. Interaction of GABAARs with CAML depends on both the cytoplasmic region and fourth transmembrane domain of the γ2 subunit, CAML immunoprecipitates with GABAARs from transfected cells and brain lysates and colocalizes with γ2 in ER vesicles in soma and dendrites of neurons. CAML shRNA treatment results in reduced expression of postsynaptic GABAARs, along with significant reductions in GABA-evoked whole-cell currents and GABAergic synaptic function, while glutamatergic transmission is unaffected. Reduced surface expression of GABAARs in CAML mutant neurons is associated with selective deficits in recycling of endocytosed GABAARs to the cell surface. Our results indicate a specific role of CAML in functional expression and endocytic recycling of postsynaptic GABAARs. PMID:18424167

  6. GOLGI IN COPPER HOMEOSTASIS: A VIEW FROM THE MEMBRANE TRAFFICKING FIELD

    PubMed Central

    Polishchuk, Roman; Lutsenko, Svetlana

    2013-01-01

    Copper is essential for a variety of important biological processes as a cofactor and regulator of many enzymes. Incorporation of copper into the secreted and plasma membrane-targeted cuproenzymes takes place in Golgi, a compartment central for normal copper homeostasis. The Golgi complex harbors copper-transporting ATPases, ATP7A and ATP7B, that transfer copper from the cytosol into Golgi lumen for incorporation into copper-dependent enzymes. The Golgi complex also sends these ATPases to appropriate post-Golgi destinations to ensure correct Cu fluxes in the body and to avoid potentially toxic copper accumulation. Mutations in ATP7A or ATP7B or in the proteins that regulate their trafficking affect their exit from Golgi or subsequent retrieval to this organelle. This, in turn, disrupts the homeostatic Cu balance, resulting in copper deficiency (Menkes disease) or copper overload (Wilson disease). Research over the last decade has yielded significant insights into the enzymatic properties and cell biology of the copper-ATPases. However, the mechanisms through which the Golgi regulates trafficking of ATP7A/7B and, therefore, maintain Cu homeostasis remain unclear. This review summarizes current data on the role of the Golgi in Cu metabolism and outlines questions and challenges that should be addressed to understand ATP7A and ATP7B trafficking mechanisms in health and disease. PMID:23846821

  7. Membrane trafficking in higher plant cells: GFP and antibodies, partners for probing the secretory pathway.

    PubMed

    Satiat-Jeunemaitre, B; Boevink, P; Hawes, C

    1999-06-01

    Eukaryotic cells are characterised by the organised distribution of membrane bounded compartments in their cytoplasm. The endoplasmic reticulum (ER) and the Golgi apparatus (GA) are part of this endomembrane machinery. They are involved in protein flow, and are in charge of specific functions such as the assembly, sorting and transport of newly synthesised proteins, glycoproteins or polysaccharides to their final destination, where the macromolecules are recognised either for action, storage, deposition or degradation. The structural and functional relationship between the ER and GA in higher plants is still a matter of debate. Therefore, it was essential to develop probes that would specifically label proteins or glycoproteins of the endomembrane system in situ. Here we compare two complementary approaches to probe plant endomembranes; immunocytochemistry on fixed cells, and in vivo studies using the expression of GFP tagged chimeric proteins. The structural relationship between ER and GA as based on pharmacological approaches using the two systems is explored.

  8. The Escrt Complexes: Structure and Mechanism of a Membrane-Trafficking Network

    PubMed Central

    Hurley, James H.; Emr, Scott D.

    2006-01-01

    The ESCRT complexes and associated proteins comprise a major pathway for the lysosomal degradation of transmembrane proteins, and are critical for receptor downregulation, budding of the HIV virus, and other normal and pathological cell processes. The ESCRT system is conserved from yeast to man. The ESCRT complexes form a network that recruits monoubiquitinated proteins and drives their internalization into lumenal vesicles within a type of endosome known as a multivesicular body. The structures and interactions of many of the components have been determined over the past three years, revealing mechanisms for membrane and cargo recruitment and for complex assembly. PMID:16689637

  9. Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase

    PubMed Central

    Cai, Ting; Wang, Haojie; Chen, Yiliang; Liu, Lijun; Gunning, William T; Quintas, Luis Eduardo M.; Xie, Zi-Jian

    2008-01-01

    Here, we show that the Na/K-ATPase interacts with caveolin-1 (Cav1) and regulates Cav1 trafficking. Graded knockdown of Na/K-ATPase decreases the plasma membrane pool of Cav1, which results in a significant reduction in the number of caveolae on the cell surface. These effects are independent of the pumping function of Na/K-ATPase, and instead depend on interaction between Na/K-ATPase and Cav1 mediated by an N-terminal caveolin-binding motif within the ATPase α1 subunit. Moreover, knockdown of the Na/K-ATPase increases basal levels of active Src and stimulates endocytosis of Cav1 from the plasma membrane. Microtubule-dependent long-range directional trafficking in Na/K-ATPase–depleted cells results in perinuclear accumulation of Cav1-positive vesicles. Finally, Na/K-ATPase knockdown has no effect on processing or exit of Cav1 from the Golgi. Thus, the Na/K-ATPase regulates Cav1 endocytic trafficking and stabilizes the Cav1 plasma membrane pool. PMID:18794328

  10. Membrane trafficking in protozoa SNARE proteins, H+-ATPase, actin, and other key players in ciliates.

    PubMed

    Plattner, Helmut

    2010-01-01

    Due to their well-defined pathways of vesicle trafficking and manyfold mutants ciliates have served as good model systems. Further studies required the development of databases, now available for Paramecium and Tetrahymena. A variety of key players have been identified and characterized based on BLAST search, domain analysis, localization, and gene-silencing studies. They include NSF (N-ethylmaleimide sensitive factor), SNAREs (soluble NSF attachment protein [SNAP] receptors), the H(+)-ATPase (V-ATPase) and actin, while Arf (ADP-ribosylation factor) and Rab-type small GTPases, COPs (coatamer proteins) and many others remain to be elucidated. The number of SNAREs, H(+)-ATPase subunits, and actins ever found within one cell type are unexpectedly high and most of the manifold vesicle types seem to be endowed with specific molecular components pertinent to trafficking. As in higher eukaryotes, multifactorial targeting likely occurs. It appears that, in parallel to higher organisms, ciliates have evolved a similar structural and molecular complexity of vesicle trafficking.

  11. ER arrival sites for COPI vesicles localize to hotspots of membrane trafficking.

    PubMed

    Schröter, Saskia; Beckmann, Sabrina; Schmitt, Hans Dieter

    2016-09-01

    COPI-coated vesicles mediate retrograde membrane traffic from the cis-Golgi to the endoplasmic reticulum (ER) in all eukaryotic cells. However, it is still unknown whether COPI vesicles fuse everywhere or at specific sites with the ER membrane. Taking advantage of the circumstance that the vesicles still carry their coat when they arrive at the ER, we have visualized active ER arrival sites (ERAS) by monitoring contact between COPI coat components and the ER-resident Dsl tethering complex using bimolecular fluorescence complementation (BiFC). ERAS form punctate structures near Golgi compartments, clearly distinct from ER exit sites. Furthermore, ERAS are highly polarized in an actin and myosin V-dependent manner and are localized near hotspots of plasma membrane expansion. Genetic experiments suggest that the COPI•Dsl BiFC complexes recapitulate the physiological interaction between COPI and the Dsl complex and that COPI vesicles are mistargeted in dsl1 mutants. We conclude that the Dsl complex functions in confining COPI vesicle fusion sites. © 2016 The Authors.

  12. Multifaceted Roles of ALG-2 in Ca2+-Regulated Membrane Trafficking

    PubMed Central

    Maki, Masatoshi; Takahara, Terunao; Shibata, Hideki

    2016-01-01

    ALG-2 (gene name: PDCD6) is a penta-EF-hand Ca2+-binding protein and interacts with a variety of proteins in a Ca2+-dependent fashion. ALG-2 recognizes different types of identified motifs in Pro-rich regions by using different hydrophobic pockets, but other unknown modes of binding are also used for non-Pro-rich proteins. Most ALG-2-interacting proteins associate directly or indirectly with the plasma membrane or organelle membranes involving the endosomal sorting complex required for transport (ESCRT) system, coat protein complex II (COPII)-dependent ER-to-Golgi vesicular transport, and signal transduction from membrane receptors to downstream players. Binding of ALG-2 to targets may induce conformational change of the proteins. The ALG-2 dimer may also function as a Ca2+-dependent adaptor to bridge different partners and connect the subnetwork of interacting proteins. PMID:27571067

  13. Linear diffusion-wave channel routing using a discrete Hayami convolution method

    NASA Astrophysics Data System (ADS)

    Wang, Li; Wu, Joan Q.; Elliot, William J.; Fiedler, Fritz R.; Lapin, Sergey

    2014-02-01

    The convolution of an input with a response function has been widely used in hydrology as a means to solve various problems analytically. Due to the high computation demand in solving the functions using numerical integration, it is often advantageous to use the discrete convolution instead of the integration of the continuous functions. This approach greatly reduces the amount of the computational work; however, it increases the possibility for mass balance errors. In this study, we analyzed the characteristics of the kernel function for the Hayami convolution solution to the linear diffusion-wave channel routing with distributed lateral inflow. We propose two ways of selection of the discrete kernel function values: using the exact point values or using the center-averaged values. Through a hypothetical example and the applications to Asotin Creek, WA and the Clearwater River, ID, we showed that when the point kernel function values were used in the discrete Hayami convolution (DHC) solution, the mass balance error of channel routing is dependent on the number of time steps on the rising limb of the Hayami kernel function. The mass balance error is negligible when there are more than 1.8 time steps on the rising limb of the kernel function. The fewer time steps on the rising limb, the greater risk of high mass balance errors. When the average kernel function values are used for the DHC solution, however, the mass balance is always maintained, since the integration of the discrete kernel function is always unity.

  14. Stepped inlet optical panel

    DOEpatents

    Veligdan, James T.

    2001-01-01

    An optical panel includes stacked optical waveguides having stepped inlet facets collectively defining an inlet face for receiving image light, and having beveled outlet faces collectively defining a display screen for displaying the image light channeled through the waveguides by internal reflection.

  15. The Seven Step Strategy

    ERIC Educational Resources Information Center

    Schaffer, Connie

    2017-01-01

    Many well-intended instructors use Socratic or leveled questioning to facilitate the discussion of an assigned reading. While this engages a few students, most can opt to remain silent. The seven step strategy described in this article provides an alternative to classroom silence and engages all students. Students discuss a single reading as they…

  16. Running Parallel Discrete Event Simulators on Sierra

    SciTech Connect

    Barnes, P. D.; Jefferson, D. R.

    2015-12-03

    In this proposal we consider porting the ROSS/Charm++ simulator and the discrete event models that run under its control so that they run on the Sierra architecture and make efficient use of the Volta GPUs.

  17. The discrete-time compensated Kalman filter

    NASA Technical Reports Server (NTRS)

    Lee, W. H.; Athans, M.

    1978-01-01

    A suboptimal dynamic compensator to be used in conjunction with the ordinary discrete time Kalman filter was derived. The resultant compensated Kalman Filter has the property that steady state bias estimation errors, resulting from modelling errors, were eliminated.

  18. Commutation Relations and Discrete Garnier Systems

    NASA Astrophysics Data System (ADS)

    Ormerod, Christopher M.; Rains, Eric M.

    2016-11-01

    We present four classes of nonlinear systems which may be considered discrete analogues of the Garnier system. These systems arise as discrete isomonodromic deformations of systems of linear difference equations in which the associated Lax matrices are presented in a factored form. A system of discrete isomonodromic deformations is completely determined by commutation relations between the factors. We also reparameterize these systems in terms of the image and kernel vectors at singular points to obtain a separate birational form. A distinguishing feature of this study is the presence of a symmetry condition on the associated linear problems that only appears as a necessary feature of the Lax pairs for the least degenerate discrete Painlevé equations.

  19. Causal fermions in discrete space-time

    NASA Astrophysics Data System (ADS)

    Farrelly, Terence C.; Short, Anthony J.

    2014-01-01

    In this paper, we consider fermionic systems in discrete space-time evolving with a strict notion of causality, meaning they evolve unitarily and with a bounded propagation speed. First, we show that the evolution of these systems has a natural decomposition into a product of local unitaries, which also holds if we include bosons. Next, we show that causal evolution of fermions in discrete space-time can also be viewed as the causal evolution of a lattice of qubits, meaning these systems can be viewed as quantum cellular automata. Following this, we discuss some examples of causal fermionic models in discrete space-time that become interesting physical systems in the continuum limit: Dirac fermions in one and three spatial dimensions, Dirac fields, and briefly the Thirring model. Finally, we show that the dynamics of causal fermions in discrete space-time can be efficiently simulated on a quantum computer.

  20. Vortex chains travelling with discrete velocities

    NASA Astrophysics Data System (ADS)

    Malishevskii, A. S.; Silin, V. P.; Uryupin, S. A.; Uspenskii, S. G.

    2008-05-01

    It has been shown that Swihart waves slowing down caused by Josephson junction spatial dispersion leads to the new field periodic nonlinear vortex states moving with discrete velocities. Swihart waves trapping by periodic vortex structures is discovered.

  1. Discrete breathers in nonlinear magnetic metamaterials.

    PubMed

    Lazarides, N; Eleftheriou, M; Tsironis, G P

    2006-10-13

    Magnetic metamaterials composed of split-ring resonators or U-type elements may exhibit discreteness effects in THz and optical frequencies due to weak coupling. We consider a model one-dimensional metamaterial formed by a discrete array of nonlinear split-ring resonators where each ring interacts with its nearest neighbors. On-site nonlinearity and weak coupling among the individual array elements result in the appearance of discrete breather excitations or intrinsic localized modes, both in the energy-conserved and the dissipative system. We analyze discrete single and multibreather excitations, as well as a special breather configuration forming a magnetization domain wall and investigate their mobility and the magnetic properties their presence induces in the system.

  2. Motion of Discrete Interfaces Through Mushy Layers

    NASA Astrophysics Data System (ADS)

    Braides, Andrea; Solci, Margherita

    2016-08-01

    We study the geometric motion of sets in the plane derived from the homogenization of discrete ferromagnetic energies with weak inclusions. We show that the discrete sets are composed by a `bulky' part and an external `mushy region' composed only of weak inclusions. The relevant motion is that of the bulky part, which asymptotically obeys to a motion by crystalline mean curvature with a forcing term, due to the energetic contribution of the mushy layers, and pinning effects, due to discreteness. From an analytical standpoint, it is interesting to note that the presence of the mushy layers implies only a weak and not strong convergence of the discrete motions, so that the convergence of the energies does not commute with the evolution. From a mechanical standpoint it is interesting to note the geometrical similarity of some phenomena in the cooling of binary melts.

  3. Radix Representation of Triangular Discrete Grid System

    NASA Astrophysics Data System (ADS)

    Ben, J.; Li, Y. L.; Wang, R.

    2016-11-01

    Discrete Global Grid Systems (DGGSs) are spatial references that use a hierarchical tessellation of cells to partition and address the entire globe. It provides an organizational structure that permits fast integration between multiple sources of large and variable geospatial data. Although many endeavors have been done to describe certain discrete grid systems, there still lack of a uniform mathematical framework for them. This paper simplifies the planar class I aperture 4 triangular discrete grid system into a hierarchical lattice model which is proved to be a radix system in the complex number plane. Mathematical properties of the radix system reveal the discrete grid system is equivalent to the set of complex numbers with special form. The conclusion provides a potential way to build a uniform mathematical framework of DGGS and can be used to design efficient encoding and spatial operation scheme for DGGS.

  4. A Few Continuous and Discrete Dynamical Systems

    NASA Astrophysics Data System (ADS)

    Zhang, Yufeng; Rui, Wenjuan

    2016-08-01

    Starting from a 2-unimodular group, we construct its new Lie algebras for which the positive-order Lax pairs and the negative-order Lax pairs are introduced, respectively. With the help of the resulting structure equation of the group we generate some partial differential equations including the well-known MKdV equation, the sine-Gordon equation, the hyperbolic sine-Gordon equation and other new nonlinear evolution equations. With the aid of the Tu scheme combined with the given Lax pairs, we obtain the isospectral and nonisospectral hierarchies of evolution equations, from which we generate two sets of symmetries of a generalized nonlinear Schrödinger (gNLS) equation. Finally, we discretize the Lax pairs to obtain a set of coupled semi-discrete equations. As their reduction, we produce the semi-discrete MKdV equation and semi-discrete NLS equation.

  5. Comparing the Discrete and Continuous Logistic Models

    ERIC Educational Resources Information Center

    Gordon, Sheldon P.

    2008-01-01

    The solutions of the discrete logistic growth model based on a difference equation and the continuous logistic growth model based on a differential equation are compared and contrasted. The investigation is conducted using a dynamic interactive spreadsheet. (Contains 5 figures.)

  6. Comparing the Discrete and Continuous Logistic Models

    ERIC Educational Resources Information Center

    Gordon, Sheldon P.

    2008-01-01

    The solutions of the discrete logistic growth model based on a difference equation and the continuous logistic growth model based on a differential equation are compared and contrasted. The investigation is conducted using a dynamic interactive spreadsheet. (Contains 5 figures.)

  7. Dynamic discretization method for solving Kepler's equation

    NASA Astrophysics Data System (ADS)

    Feinstein, Scott A.; McLaughlin, Craig A.

    2006-09-01

    Kepler’s equation needs to be solved many times for a variety of problems in Celestial Mechanics. Therefore, computing the solution to Kepler’s equation in an efficient manner is of great importance to that community. There are some historical and many modern methods that address this problem. Of the methods known to the authors, Fukushima’s discretization technique performs the best. By taking more of a system approach and combining the use of discretization with the standard computer science technique known as dynamic programming, we were able to achieve even better performance than Fukushima. We begin by defining Kepler’s equation for the elliptical case and describe existing solution methods. We then present our dynamic discretization method and show the results of a comparative analysis. This analysis will demonstrate that, for the conditions of our tests, dynamic discretization performs the best.

  8. Terminal Dynamics Approach to Discrete Event Systems

    NASA Technical Reports Server (NTRS)

    Zak, Michail; Meyers, Ronald

    1995-01-01

    This paper presents and discusses a mathematical formalism for simulation of discrete event dynamic (DED)-a special type of 'man-made' systems to serve specific purposes of information processing. The main objective of this work is to demonstrate that the mathematical formalism for DED can be based upon a terminal model of Newtonian dynamics which allows one to relax Lipschitz conditions at some discrete points.!.

  9. Discrete Surface Modelling Using Partial Differential Equations.

    PubMed

    Xu, Guoliang; Pan, Qing; Bajaj, Chandrajit L

    2006-02-01

    We use various nonlinear partial differential equations to efficiently solve several surface modelling problems, including surface blending, N-sided hole filling and free-form surface fitting. The nonlinear equations used include two second order flows, two fourth order flows and two sixth order flows. These nonlinear equations are discretized based on discrete differential geometry operators. The proposed approach is simple, efficient and gives very desirable results, for a range of surface models, possibly having sharp creases and corners.

  10. Development of discrete components. Final report

    SciTech Connect

    Brown, R.J.

    1995-11-01

    Allied-Signal Inc, Kansas City Division, was provided with funding to maintain the capability to procure discrete components for various applications. A development project was undertaken to procure transistor die from one supplier for assembly into finished components by a different supplier. These components would be SA-equivalent with appropriate preconditioning, testing, and certification, The methodologies developed herein go far to ensure the future availability of discrete components.

  11. Discrete mechanics, "time machines" and hybrid systems

    NASA Astrophysics Data System (ADS)

    Elze, Hans-Thomas

    2013-09-01

    Modifying the discrete mechanics proposed by T.D. Lee, we construct a class of discrete classical Hamiltonian systems, in which time is one of the dynamical variables. This includes a toy model of "time machines" which can travel forward and backward in time and which differ from models based on closed timelike curves (CTCs). In the continuum limit, we explore the interaction between such time reversing machines and quantum mechanical objects, employing a recent description of quantum-classical hybrids.

  12. Creative Visualization in Discrete Global Grid System

    NASA Astrophysics Data System (ADS)

    Etemad, K.; Samavati, F.; Sherlock, M. J.

    2016-12-01

    Discrete Global Grid System (DGGS) is a disruptive method for developing digital representation of the Earth. In DGGS, to create a multiresolution representation of the Earth, the surface of the Earth is discretized to a hierarchy of indexed (mostly) regular cells. In this talk, an overview of research projects and recent achievements from my group related to DGGS is provided. This covers example works in large geospatial data processing and streaming, as well as creative visualization and interaction in the context of DGGS.

  13. Recent advances in discrete dipole approximation

    NASA Astrophysics Data System (ADS)

    Flatau, P. J.

    2012-12-01

    I will describe recent advances and results related to Discrete Dipole Approximation. I will concentrate on Discrete Dipole Scattering (DDSCAT) code which has been jointly developed by myself and Bruce T. Draine. Discussion will concentrate on calculation of scattering and absorption by isolated particles (e.g., dust grains, ice crystals), calculations of scattering by periodic structures with applications to studies of scattering and absorption by periodic arrangement of finite cylinders, cubes, etc), very fast near field calculation, ways to display scattering targets and their composition using three dimensional graphical codes. I will discuss possible extensions. References Flatau, P. J. and Draine, B. T., 2012, Fast near field calculations in the discrete dipole approximation for regular rectilinear grids, Optics Express, 20, 1247-1252. Draine B. T. and Flatau P. J., 2008, Discrete-dipole approximation for periodic targets: theory and tests , J. Opt. Soc. Am. A., 25, 2693-2703. Draine BT and Flatau PJ, 2012, User Guide for the Discrete Dipole Approximation Code DDSCAT 7.2, arXiv:1202.3424v3.ear field calculations (Fast near field calculations in the discrete dipole approximation for regular rectilinear grids P. J. Flatau and B. T. Draine, Optics Express, Vol. 20, Issue 2, pp. 1247-1252 (2012))

  14. Discretization of continuous features in clinical datasets

    PubMed Central

    Maslove, David M; Podchiyska, Tanya; Lowe, Henry J

    2013-01-01

    Background The increasing availability of clinical data from electronic medical records (EMRs) has created opportunities for secondary uses of health information. When used in machine learning classification, many data features must first be transformed by discretization. Objective To evaluate six discretization strategies, both supervised and unsupervised, using EMR data. Materials and methods We classified laboratory data (arterial blood gas (ABG) measurements) and physiologic data (cardiac output (CO) measurements) derived from adult patients in the intensive care unit using decision trees and naïve Bayes classifiers. Continuous features were partitioned using two supervised, and four unsupervised discretization strategies. The resulting classification accuracy was compared with that obtained with the original, continuous data. Results Supervised methods were more accurate and consistent than unsupervised, but tended to produce larger decision trees. Among the unsupervised methods, equal frequency and k-means performed well overall, while equal width was significantly less accurate. Discussion This is, we believe, the first dedicated evaluation of discretization strategies using EMR data. It is unlikely that any one discretization method applies universally to EMR data. Performance was influenced by the choice of class labels and, in the case of unsupervised methods, the number of intervals. In selecting the number of intervals there is generally a trade-off between greater accuracy and greater consistency. Conclusions In general, supervised methods yield higher accuracy, but are constrained to a single specific application. Unsupervised methods do not require class labels and can produce discretized data that can be used for multiple purposes. PMID:23059731

  15. Discrete mappings with an explicit discrete Lyapunov function related to integrable mappings

    NASA Astrophysics Data System (ADS)

    Inoue, Hironori; Takahashi, Daisuke; Matsukidaira, Junta

    2006-05-01

    We propose discrete mappings of second order that have a discrete analogue of Lyapunov function. The mappings are extensions of the integrable Quispel-Roberts-Thompson (QRT) mapping, and a discrete Lyapunov function of the mappings is identical to an explicit conserved quantity of the QRT mapping. Moreover we can obtain a differential and an ultradiscrete limit of the mappings preserving the existence of Lyapunov function. We also give applications of a mapping with an adjusted parameter, a probabilistic mapping and coupled mappings.

  16. Micromachine Wedge Stepping Motor

    SciTech Connect

    Allen, J.J.; Schriner, H.K.

    1998-11-04

    A wedge stepping motor, which will index a mechanism, has been designed and fabricated in the surface rnicromachine SUMMiT process. This device has demonstrated the ability to index one gear tooth at a time with speeds up to 205 teeth/see. The wedge stepper motor has the following features, whi:h will be useful in a number of applications. o The ability to precisely position mechanical components. . Simple pulse signals can be used for operation. o Only 2 drive signals are requixed for operation. o Torque and precision capabilities increase with device size . The device to be indexed is restrained at all times by the wedge shaped tooth that is used for actuation. This paper will discuss the theory of operation and desi=m of the wedge stepping motor. The fabrication and testing of I he device will also be presented.

  17. The digital step edge

    NASA Technical Reports Server (NTRS)

    Haralick, R. M.

    1982-01-01

    The facet model was used to accomplish step edge detection. The essence of the facet model is that any analysis made on the basis of the pixel values in some neighborhood has its final authoritative interpretation relative to the underlying grey tone intensity surface of which the neighborhood pixel values are observed noisy samples. Pixels which are part of regions have simple grey tone intensity surfaces over their areas. Pixels which have an edge in them have complex grey tone intensity surfaces over their areas. Specially, an edge moves through a pixel only if there is some point in the pixel's area having a zero crossing of the second directional derivative taken in the direction of a non-zero gradient at the pixel's center. To determine whether or not a pixel should be marked as a step edge pixel, its underlying grey tone intensity surface was estimated on the basis of the pixels in its neighborhood.

  18. Step Prototype Development Status

    NASA Astrophysics Data System (ADS)

    Mehls, C.; Bayart, C.; Bower, J.; Clarke, B.; Cox, C.; Gill, D.; Stricker, D.; Vora, N.; Wang, S.; Zhou, P.; Torii, R.; Worden, P.; Debra, D.; Dittus, H.; Loeffler, F.

    2008-09-01

    STEP, the Satellite Test of the Equivalence Principle [1], proposes to test the Equivalence Principle to a part in 1018 by comparing the free-fall acceleration of cylindrical shaped test masses [2] in Earth orbit. Magnetic bearings constrain the test mass motion to their axis of symmetry [3]. The displacement of the test masses is measured using a DC SQUID and superconducting coils [4], enabling a displacement sensitivity as small as 10-15 m. In combination with a small spring stiffness a differential acceleration sensitivity of 10-18 g is achievable. Residual satellite acceleration is reduced to better than 10-14 g by compensating satellite drag forces with thrust provided by helium gas. We report on recent progress in the development of STEP prototype flight accelerometers, in particular the development of the high precision quartz housing for the engineering inner accelerometer and the testing of SQUID and capacitive readout systems using 'brass board' accelerometer prototypes.

  19. SIRTF: The next step

    NASA Technical Reports Server (NTRS)

    Gillett, F. C.; Werner, M. W.

    1984-01-01

    The scientific and technical background and prospects for the space infrared telescope facility (SIRTF) are described. This facility is a superfluid-helium-cooled, 0.85-meter infrared telescope to be placed in orbit in 1993. It is designed to carry out photometry over the wavelength range 2 to 700 micrometers, and diffraction-limited imagery in either broad or narrow spectral bands over the range 1.8 to 200 micrometers. It is proposed that SIRTF measure spectra in the range 2.5 to 200 micrometers with resolving power between 50 and 1000 and that the focal plane contain about 20,000 detector elements, both discrete and in arrays. The SIRTF observatory is designed to be a long-lived facility providing opportunities for general investigations by the entire scientific community. For following up the all-sky survey carried out by the Infrared Astronomical Satellite (IRAS), SIRTF is ideal. It can do a deep survey to flux levels 5000 times fainer than IRAS and can obtain spectra of even the faintest IRAS sources.

  20. On the consistency between nearest-neighbor peridynamic discretizations and discretized classical elasticity models

    DOE PAGES

    Seleson, Pablo; Du, Qiang; Parks, Michael L.

    2016-08-16

    The peridynamic theory of solid mechanics is a nonlocal reformulation of the classical continuum mechanics theory. At the continuum level, it has been demonstrated that classical (local) elasticity is a special case of peridynamics. Such a connection between these theories has not been extensively explored at the discrete level. This paper investigates the consistency between nearest-neighbor discretizations of linear elastic peridynamic models and finite difference discretizations of the Navier–Cauchy equation of classical elasticity. While nearest-neighbor discretizations in peridynamics have been numerically observed to present grid-dependent crack paths or spurious microcracks, this paper focuses on a different, analytical aspect of suchmore » discretizations. We demonstrate that, even in the absence of cracks, such discretizations may be problematic unless a proper selection of weights is used. Specifically, we demonstrate that using the standard meshfree approach in peridynamics, nearest-neighbor discretizations do not reduce, in general, to discretizations of corresponding classical models. We study nodal-based quadratures for the discretization of peridynamic models, and we derive quadrature weights that result in consistency between nearest-neighbor discretizations of peridynamic models and discretized classical models. The quadrature weights that lead to such consistency are, however, model-/discretization-dependent. We motivate the choice of those quadrature weights through a quadratic approximation of displacement fields. The stability of nearest-neighbor peridynamic schemes is demonstrated through a Fourier mode analysis. Finally, an approach based on a normalization of peridynamic constitutive constants at the discrete level is explored. This approach results in the desired consistency for one-dimensional models, but does not work in higher dimensions. The results of the work presented in this paper suggest that even though nearest

  1. PREFACE: 4th Symposium on Prospects in the Physics of Discrete Symmetries (DISCRETE2014)

    NASA Astrophysics Data System (ADS)

    Di Domenico, Antonio; Mavromatos, Nick E.; Mitsou, Vasiliki A.; Skliros, Dimitri P.

    2015-07-01

    The DISCRETE 2014: Fourth Symposium in the Physics of Discrete Symmetries took place at King's College London, Strand Campus, London WC2R 2LS, from Tuesday, December 2 2014 till Saturday, December 6 2014. This is the fourth Edition of the DISCRETE conference series, which is a biannual event, having been held previously in Valencia (Discrete'08), Rome (Discrete2010) and Lisbon (Discrete2012). The topics covered at the DISCRETE series of conferences are: T, C, P, CP symmetries; accidental symmetries (B, L conservation); CPT symmetry, decoherence and entangled states, Lorentz symmetry breaking (phenomenology and current bounds); neutrino mass and mixing; implications for cosmology and astroparticle physics, dark matter searches; experimental prospects at LHC, new facilities. In DISCRETE 2014 we have also introduced two new topics: cosmological aspects of non-commutative space-times as well as PT symmetric Hamiltonians (non-Hermitian but with real eigenvalues), a topic that has wide applications in particle physics and beyond. The conference was opened by the King's College London Vice Principal on Research and Innovation, Mr Chris Mottershead, followed by a welcome address by the Chair of DISCRETE 2014 (Professor Nick E. Mavromatos). After these introductory talks, the scientific programme of the DISCRETE 2014 symposium started. Following the tradition of DISCRETE series of conferences, the talks (138 in total) were divided into plenary-review talks (25), invited research talks (50) and shorter presentations (63) — selected by the conveners of each session in consultation with the organisers — from the submitted abstracts. We have been fortunate to have very high-quality, thought stimulating and interesting talks at all levels, which, together with the discussions among the participants, made the conference quite enjoyable. There were 152 registered participants for the event.

  2. Multi-Target Tracking by Discrete-Continuous Energy Minimization.

    PubMed

    Milan, Anton; Schindler, Konrad; Roth, Stefan

    2016-10-01

    The task of tracking multiple targets is often addressed with the so-called tracking-by-detection paradigm, where the first step is to obtain a set of target hypotheses for each frame independently. Tracking can then be regarded as solving two separate, but tightly coupled problems. The first is to carry out data association, i.e., to determine the origin of each of the available observations. The second problem is to reconstruct the actual trajectories that describe the spatio-temporal motion pattern of each individual target. The former is inherently a discrete problem, while the latter should intuitively be modeled in continuous space. Having to deal with an unknown number of targets, complex dependencies, and physical constraints, both are challenging tasks on their own and thus most previous work focuses on one of these subproblems. Here, we present a multi-target tracking approach that explicitly models both tasks as minimization of a unified discrete-continuous energy function. Trajectory properties are captured through global label costs, a recent concept from multi-model fitting, which we introduce to tracking. Specifically, label costs describe physical properties of individual tracks, e.g., linear and angular dynamics, or entry and exit points. We further introduce pairwise label costs to describe mutual interactions between targets in order to avoid collisions. By choosing appropriate forms for the individual energy components, powerful discrete optimization techniques can be leveraged to address data association, while the shapes of individual trajectories are updated by gradient-based continuous energy minimization. The proposed method achieves state-of-the-art results on diverse benchmark sequences.

  3. An Improved Manufacturing Approach for Discrete Silicon Microneedle Arrays with Tunable Height-Pitch Ratio

    PubMed Central

    Wang, Renxin; Wang, Wei; Li, Zhihong

    2016-01-01

    Silicon microneedle arrays (MNAs) have been widely studied due to their potential in various transdermal applications. However, discrete MNAs, as a preferred choice to fabricate flexible penetrating devices that could adapt curved and elastic tissue, are rarely reported. Furthermore, the reported discrete MNAs have disadvantages lying in uniformity and height-pitch ratio. Therefore, an improved technique is developed to manufacture discrete MNA with tunable height-pitch ratio, which involves KOH-dicing-KOH process. The detailed process is sketched and simulated to illustrate the formation of microneedles. Furthermore, the undercutting of convex mask in two KOH etching steps are mathematically analyzed, in order to reveal the relationship between etching depth and mask dimension. Subsequently, fabrication results demonstrate KOH-dicing-KOH process. {321} facet is figured out as the surface of octagonal pyramid microneedle. MNAs with diverse height and pitch are also presented to identify the versatility of this approach. At last, the metallization is realized via successive electroplating. PMID:27735837

  4. An Improved Manufacturing Approach for Discrete Silicon Microneedle Arrays with Tunable Height-Pitch Ratio.

    PubMed

    Wang, Renxin; Wang, Wei; Li, Zhihong

    2016-10-09

    Silicon microneedle arrays (MNAs) have been widely studied due to their potential in various transdermal applications. However, discrete MNAs, as a preferred choice to fabricate flexible penetrating devices that could adapt curved and elastic tissue, are rarely reported. Furthermore, the reported discrete MNAs have disadvantages lying in uniformity and height-pitch ratio. Therefore, an improved technique is developed to manufacture discrete MNA with tunable height-pitch ratio, which involves KOH-dicing-KOH process. The detailed process is sketched and simulated to illustrate the formation of microneedles. Furthermore, the undercutting of convex mask in two KOH etching steps are mathematically analyzed, in order to reveal the relationship between etching depth and mask dimension. Subsequently, fabrication results demonstrate KOH-dicing-KOH process. {321} facet is figured out as the surface of octagonal pyramid microneedle. MNAs with diverse height and pitch are also presented to identify the versatility of this approach. At last, the metallization is realized via successive electroplating.

  5. Constant pressure and temperature discrete-time Langevin molecular dynamics

    SciTech Connect

    Grønbech-Jensen, Niels; Farago, Oded

    2014-11-21

    We present a new and improved method for simultaneous control of temperature and pressure in molecular dynamics simulations with periodic boundary conditions. The thermostat-barostat equations are built on our previously developed stochastic thermostat, which has been shown to provide correct statistical configurational sampling for any time step that yields stable trajectories. Here, we extend the method and develop a set of discrete-time equations of motion for both particle dynamics and system volume in order to seek pressure control that is insensitive to the choice of the numerical time step. The resulting method is simple, practical, and efficient. The method is demonstrated through direct numerical simulations of two characteristic model systems—a one-dimensional particle chain for which exact statistical results can be obtained and used as benchmarks, and a three-dimensional system of Lennard-Jones interacting particles simulated in both solid and liquid phases. The results, which are compared against the method of Kolb and Dünweg [J. Chem. Phys. 111, 4453 (1999)], show that the new method behaves according to the objective, namely that acquired statistical averages and fluctuations of configurational measures are accurate and robust against the chosen time step applied to the simulation.

  6. Discrete-element modeling of particulate aerosol flows

    SciTech Connect

    Marshall, J.S.

    2009-03-20

    A multiple-time step computational approach is presented for efficient discrete-element modeling of aerosol flows containing adhesive solid particles. Adhesive aerosol particulates are found in numerous dust and smoke contamination problems, including smoke particle transport in the lungs, particle clogging of heat exchangers in construction vehicles, industrial nanoparticle transport and filtration systems, and dust fouling of electronic systems and MEMS components. Dust fouling of equipment is of particular concern for potential human occupation on dusty planets, such as Mars. The discrete-element method presented in this paper can be used for prediction of aggregate structure and breakup, for prediction of the effect of aggregate formation on the bulk fluid flow, and for prediction of the effects of small-scale flow features (e.g., due to surface roughness or MEMS patterning) on the aggregate formation. After presentation of the overall computational structure, the forces and torques acting on the particles resulting from fluid motion, particle-particle collision, and adhesion under van der Waals forces are reviewed. The effect of various parameters of normal collision and adhesion of two particles are examined in detail. The method is then used to examine aggregate formation and particle clogging in pipe and channel flow.

  7. Quantum Perturbative Approach to Discrete Redshift

    NASA Astrophysics Data System (ADS)

    Roberts, Mark D.

    On the largest scales there is evidence of discrete structure, examples of this are superclusters and voids and also by redshift taking discrete values. In this paper it is proposed that discrete redshift can be explained by using the spherical harmonic integer l; this occurs both in the metric or density perturbations and also in the solution of wave equations in Robertson-Walker spacetime. It is argued that the near conservation of energy implies that l varies regularly for wave equations in Robertson-Walker spacetime, whereas for density perturbations l cannot vary regularly. Once this is assumed then perhaps the observed value of discrete redshift provides the only observational or experimental data that directly requires an explanation using both gravitational and quantum theory. In principle a model using this data could predict the scale factor R (or equivalently the deceleration parameter q). Solutions of the Klein-Gordon equation in Robertson-Walker spacetimes are used to devise models which have redshift taking discrete values, but they predict a microscopic value for R. A model in which the stress of the Klein-Gordon equation induces a metrical perturbation of Robertson-Walker spacetime is devised. Calculations based upon this model predict that the Universe is closed with 2_q0 - 1=10^-4.

  8. Discrete breathers in graphane in thermal equilibrium

    NASA Astrophysics Data System (ADS)

    Baimova, J. A.; Murzaev, R. T.; Rudskoy, A. I.

    2017-09-01

    Nonlinear dynamics of graphane (hydrogenated graphene) as well as some other properties of this new promising material are of high interest nowadays. One of the main challenges is the explanation of hydrogenation/dehydrogenation process of graphane at finite temperatures and the understanding of the underlying mechanisms. In present work, the hypothesis of discrete breathers working as the activators of the dehydrogenation is presented. Molecular dynamics simulation is conducted to study the discrete breathers in graphane in thermal equilibrium for temperature range 50-600 K. With the temperature increase the possibility of atom separation decreases because of thermal oscillations while the critical amplitude of the atom separation increase. It is shown that nonlinear localized modes or discrete breathers can be found in graphane at thermal equilibrium at temperature range of 400-600 K. The lifetime of discrete breather increases with the increase of its initial amplitude while temperature decrease leads to the increase of lifetime. It is concluded, that discrete breathers can facilitate the process of graphene dehydrogenation, because of their high energy.

  9. Step proof mass dynamics

    NASA Astrophysics Data System (ADS)

    Wiegand, M.; Scheithauer, S.; Theil, S.

    2004-05-01

    The Satellite Test of Equivalence Principle (STEP) is a joint European-US project to investigate one of the most fundamental principles in physics, the Equivalence of inertia and passive gravitational mass. As STEP matures into a flight program, the development of a precise spacecraft dynamics simulator becomes crucial. The simulator is primarily needed for design, test and verification of the drag-free control (DFC) system and the flight software. The drag-free concept involves centering the proof mass located inside a satellite. As the proof mass is free of external disturbances (drag free), it follows a purely gravitational orbit. Since the satellite is forced to follow the proof mass, it too follows the same gravitational orbit, canceling all non-gravitational forces. For the STEP Mission, the DFC system is required to attenuate any disturbance forces acting on the spacecraft to achieve residual acceleration at location of the accelerometer of less than 3×10 -14 m/s2 (rms) across the measurement bandwidth. While the simulator is based on a high-fidelity six-degree-of-freedom numerical simulation, a simplified model is used to analyze the proof mass dynamics. The stability analysis of the proof mass motion is performed by transformation of the simplified model into the standard form of the Mathieu differential equation. The stability regions of the solution are applied to choose proper values for parameters like coupling forces between satellite and proof mass as a function of spacecraft rotation. The paper describes the calculation of the spacecraft/payload dynamics and the assumptions used to derive the underlying algorithms with a special emphasis on numerical precision issues.

  10. Discrete Mathematics in the Schools. DIMACS Series in Discrete Mathematics and Theoretical Computer Science, Volume 36.

    ERIC Educational Resources Information Center

    Rosenstein, Joseph G., Ed.; Franzblau, Deborah S., Ed.; Roberts, Fred S., Ed.

    This book is a collection of articles by experienced educators and explains why and how discrete mathematics should be taught in K-12 classrooms. It includes evidence for "why" and practical guidance for "how" and also discusses how discrete mathematics can be used as a vehicle for achieving the broader goals of the major…

  11. Discrete breathers for a discrete nonlinear Schrödinger ring coupled to a central site.

    PubMed

    Jason, Peter; Johansson, Magnus

    2016-01-01

    We examine the existence and properties of certain discrete breathers for a discrete nonlinear Schrödinger model where all but one site are placed in a ring and coupled to the additional central site. The discrete breathers we focus on are stationary solutions mainly localized on one or a few of the ring sites and possibly also the central site. By numerical methods, we trace out and study the continuous families the discrete breathers belong to. Our main result is the discovery of a split bifurcation at a critical value of the coupling between neighboring ring sites. Below this critical value, families form closed loops in a certain parameter space, implying that discrete breathers with and without central-site occupation belong to the same family. Above the split bifurcation the families split up into several separate ones, which bifurcate with solutions with constant ring amplitudes. For symmetry reasons, the families have different properties below the split bifurcation for even and odd numbers of sites. It is also determined under which conditions the discrete breathers are linearly stable. The dynamics of some simpler initial conditions that approximate the discrete breathers are also studied and the parameter regimes where the dynamics remain localized close to the initially excited ring site are related to the linear stability of the exact discrete breathers.

  12. Discrete Mathematics in the Schools. DIMACS Series in Discrete Mathematics and Theoretical Computer Science, Volume 36.

    ERIC Educational Resources Information Center

    Rosenstein, Joseph G., Ed.; Franzblau, Deborah S., Ed.; Roberts, Fred S., Ed.

    This book is a collection of articles by experienced educators and explains why and how discrete mathematics should be taught in K-12 classrooms. It includes evidence for "why" and practical guidance for "how" and also discusses how discrete mathematics can be used as a vehicle for achieving the broader goals of the major…

  13. Asymptotic analysis of the spatial discretization of radiation absorption and re-emission in Implicit Monte Carlo

    SciTech Connect

    Densmore, Jeffery D.

    2011-02-20

    We perform an asymptotic analysis of the spatial discretization of radiation absorption and re-emission in Implicit Monte Carlo (IMC), a Monte Carlo technique for simulating nonlinear radiative transfer. Specifically, we examine the approximation of absorption and re-emission by a spatially continuous artificial-scattering process and either a piecewise-constant or piecewise-linear emission source within each spatial cell. We consider three asymptotic scalings representing (i) a time step that resolves the mean-free time, (ii) a Courant limit on the time-step size, and (iii) a fixed time step that does not depend on any asymptotic scaling. For the piecewise-constant approximation, we show that only the third scaling results in a valid discretization of the proper diffusion equation, which implies that IMC may generate inaccurate solutions with optically large spatial cells if time steps are refined. However, we also demonstrate that, for a certain class of problems, the piecewise-linear approximation yields an appropriate discretized diffusion equation under all three scalings. We therefore expect IMC to produce accurate solutions for a wider range of time-step sizes when the piecewise-linear instead of piecewise-constant discretization is employed. We demonstrate the validity of our analysis with a set of numerical examples.

  14. A Reconfigurable Stepping Motor

    NASA Astrophysics Data System (ADS)

    Rogers, Charles; Selvaggi, Richard

    2009-04-01

    Multiphase brushless actuators, commonly known as the stepper motors, are ubiquitous for many precision control applications. Developments in the microelectronics have lead to their use as efficient drive motors for modern electric vehicles. Understanding the physics and the control logic for interfacing these transducers continues to be important for scientists and engineers. An overview of the stepping motor principles and interfacing requirements is presented and a simple working model used to teach the concepts of stepper motors is described and demonstrated. This model was used to design a much larger stepper motor required to precisely rotate a massive optical system in the undergraduate advanced physics laboratory.

  15. Steps to the moon

    USGS Publications Warehouse

    ,; Dale, Alvin E.

    1976-01-01

    On July 20, 1969, man walked on the surface of the Moon and began a new chapter of his studies that will eventually disclose the geologic nature of the Earth's nearest neighbor. Although he has finally reached the Moon and sampled its substance, much work and study remain before he will know the full scientific significance of the first landing. This booklet briefly summarizes the steps man has taken to understand the Moon and what he thinks he has learned to date as a result of his centuries-long speculations and studies.

  16. New photolithography stepping machine

    SciTech Connect

    Hale, L.; Klingmann, J.; Markle, D.

    1995-03-08

    A joint development project to design a new photolithography steeping machine capable of 150 nanometer overlay accuracy was completed by Ultratech Stepper and the Lawrence Livermore National Laboratory. The principal result of the project is a next-generation product that will strengthen the US position in step-and-repeat photolithography. The significant challenges addressed and solved in the project are the subject of this report. Design methods and new devices that have broader application to precision machine design are presented in greater detail while project specific information serves primarily as background and motivation.

  17. Impedance Matching for Discrete, Periodic Media and Application to Two-Scale Wave Propagation Models

    NASA Astrophysics Data System (ADS)

    Thirunavukkarasu, Senganal

    This dissertation introduces the idea of an equivalent continuous medium (ECM) that has the same impedance as that of an unbounded discrete periodic medium. Contrary to existing knowledge, we constructively show that it is indeed possible to achieve perfect matching for periodic and discrete media. We present analytical results relating the propagation characteristics of periodic media and the corresponding ECM, leading to the development of numerical methods for wave propagation in these media. In this dissertation, we present the main idea of ECM and apply it, with mixed results, to seemingly different problems requiring effective numerical methods for modeling wave propagation in unbounded media. An immediate application of ECM is in developing absorbing boundary conditions (ABCs) for wave propagation in unbounded discrete media. Using the idea of ECM, and building on class of continuous ABCs called perfectly matched discrete layers (PMDL), we propose a new class of discrete ABCs called discrete PMDL and develop frequency domain formulations that are shown to be superior to continuous ABCs. Another application that is explored in this dissertation is the design of interface conditions for concurrent coupling of two-scale wave propagation models, e.g. Atomistic-to-Continuum (AtC) coupling. We propose a domain-decomposition (DD) approach and develop accurate interface conditions that are critical for the concurrent coupling of the two-scale models. It turns out that time-domain discrete ABCs are key to the the accuracy of these interface conditions. Since discrete PMDL is well-posed and accurate for the model problem, we build on it to propose an efficient and accurate interface condition for two-scale wave propagation models. Although many open problems remain with respect to implementation, we believe that the proposed DD based approach is a good first step towards achieving efficient coupling of two-scale wave propagation models. Time-domain discrete PMDL can

  18. Energy Levels of 'Hydrogen Atom' in Discrete Time Dynamics

    SciTech Connect

    Khrennikov, Andrei; Volovich, Yaroslav

    2006-01-04

    We analyze dynamical consequences of a conjecture that there exists a fundamental (indivisible) quant of time. In particular we study the problem of discrete energy levels of hydrogen atom. We are able to reconstruct potential which in discrete time formalism leads to energy levels of unperturbed hydrogen atom. We also consider linear energy levels of quantum harmonic oscillator and show how they are produced in the discrete time formalism. More generally, we show that in discrete time formalism finite motion in central potential leads to discrete energy spectrum, the property which is common for quantum mechanical theory. Thus deterministic (but discrete time{exclamation_point}) dynamics is compatible with discrete energy levels.

  19. Formation of discrete solitons in light-induced photonic lattices.

    PubMed

    Chen, Zhigang; Martin, Hector; Eugenieva, Eugenia; Xu, Jingjun; Yang, Jianke

    2005-03-21

    We present both experimental and theoretical results on discrete solitons in two-dimensional optically-induced photonic lattices in a variety of settings, including fundamental discrete solitons, vector-like discrete solitons, discrete dipole solitons, and discrete soliton trains. In each case, a clear transition from two-dimensional discrete diffraction to discrete trapping is demonstrated with a waveguide lattice induced by partially coherent light in a bulk photorefractive crystal. Our experimental results are in good agreement with the theoretical analysis of these effects.

  20. Towards effective flow simulations in realistic discrete fracture networks

    NASA Astrophysics Data System (ADS)

    Berrone, Stefano; Pieraccini, Sandra; Scialò, Stefano

    2016-04-01

    We focus on the simulation of underground flow in fractured media, modeled by means of Discrete Fracture Networks. Focusing on a new recent numerical approach proposed by the authors for tackling the problem avoiding mesh generation problems, we further improve the new family of methods making a step further towards effective simulations of large, multi-scale, heterogeneous networks. Namely, we tackle the imposition of Dirichlet boundary conditions in weak form, in such a way that geometrical complexity of the DFN is not an issue; we effectively solve DFN problems with fracture transmissivities spanning many orders of magnitude and approaching zero; furthermore, we address several numerical issues for improving the numerical solution also in quite challenging networks.

  1. Improved stochastic approximation methods for discretized parabolic partial differential equations

    NASA Astrophysics Data System (ADS)

    Guiaş, Flavius

    2016-12-01

    We present improvements of the stochastic direct simulation method, a known numerical scheme based on Markov jump processes which is used for approximating solutions of ordinary differential equations. This scheme is suited especially for spatial discretizations of evolution partial differential equations (PDEs). By exploiting the full path simulation of the stochastic method, we use this first approximation as a predictor and construct improved approximations by Picard iterations, Runge-Kutta steps, or a combination. This has as consequence an increased order of convergence. We illustrate the features of the improved method at a standard benchmark problem, a reaction-diffusion equation modeling a combustion process in one space dimension (1D) and two space dimensions (2D).

  2. Tuned oscillatory behavior in discrete quantum walks on star clusters

    NASA Astrophysics Data System (ADS)

    Andrade, R. F. S.; Souza, A. M. C.

    2015-10-01

    A discrete time quantum walk on the star network is considered, on which the walker has a waiting probability at any time step and for any of the N nodes. This contrasts with a previous continuous time analysis, in which the walker in any of the N -1 leaf nodes is forced to jump back to the central hub. The model amounts to considering two coin operators, one for the hub (with N possible states) and another one for all leaf nodes (with two possible states). The solution depends on N and θ , an angle gauging the action of the coin operator on the leaf nodes. Periodic solutions are identified, which can be represented as superposition of large-period branches, sharing a relative small number of shapes and displaced by a regular interval. It is shown that the large period is very sensitive to the choice of N and θ . The possibility of experimental applications of this property is briefly mentioned.

  3. ADART: an adaptive algebraic reconstruction algorithm for discrete tomography.

    PubMed

    Maestre-Deusto, F Javier; Scavello, Giovanni; Pizarro, Joaquín; Galindo, Pedro L

    2011-08-01

    In this paper we suggest an algorithm based on the Discrete Algebraic Reconstruction Technique (DART) which is capable of computing high quality reconstructions from substantially fewer projections than required for conventional continuous tomography. Adaptive DART (ADART) goes a step further than DART on the reduction of the number of unknowns of the associated linear system achieving a significant reduction in the pixel error rate of reconstructed objects. The proposed methodology automatically adapts the border definition criterion at each iteration, resulting in a reduction of the number of pixels belonging to the border, and consequently of the number of unknowns in the general algebraic reconstruction linear system to be solved, being this reduction specially important at the final stage of the iterative process. Experimental results show that reconstruction errors are considerably reduced using ADART when compared to original DART, both in clean and noisy environments.

  4. Discrete element modeling of a mining-induced rock slide.

    PubMed

    Zhao, JianJun; Xiao, JianGuo; Lee, Min Lee; Ma, YunTao

    2016-01-01

    Slopes are subjected to stress redistributions during underground mining activities, and this may eventually cause deformation or landslide. This paper takes Madaling landslide in Guizhou Province, China as a case study to investigate the failure mechanism and its run-out behaviours by using discrete element method. Previous qualitative analysis indicated that the slope experienced four stages of failure mechanisms: (1) development of tension cracks, (2) development of stepped-like creep cracks, (3) development of potential rupture surfaces, and (4) occurrence of the landslide. PFC2D program was employed to model the pre-failure deformation characteristics in order to verify the failure mechanisms quantitatively. Subsequently, the run-out behaviours of the landslide were analyzed by PFC3D program. The results indicated that the movement could be summarized into four stages: acceleration stage, constant movement stage, rapid movement stage, and deceleration and deposition stage.

  5. Quality Improvement With Discrete Event Simulation: A Primer for Radiologists.

    PubMed

    Booker, Michael T; O'Connell, Ryan J; Desai, Bhushan; Duddalwar, Vinay A

    2016-04-01

    The application of simulation software in health care has transformed quality and process improvement. Specifically, software based on discrete-event simulation (DES) has shown the ability to improve radiology workflows and systems. Nevertheless, despite the successful application of DES in the medical literature, the power and value of simulation remains underutilized. For this reason, the basics of DES modeling are introduced, with specific attention to medical imaging. In an effort to provide readers with the tools necessary to begin their own DES analyses, the practical steps of choosing a software package and building a basic radiology model are discussed. In addition, three radiology system examples are presented, with accompanying DES models that assist in analysis and decision making. Through these simulations, we provide readers with an understanding of the theory, requirements, and benefits of implementing DES in their own radiology practices.

  6. Monte Carlo integration over stepping stone models for spatial genetic inference using approximate Bayesian computation.

    PubMed

    Baird, Stuart J E; Santos, Filipe

    2010-09-01

    Approximate Bayesian computation (ABC) substitutes simulation for analytic models in Bayesian inference. Simulating evolutionary scenarios under Kimura's stepping stone model (KSS) might therefore allow inference over spatial genetic process where analytical results are difficult to obtain. ABC first creates a reference set of simulations and would proceed by comparing summary statistics over KSS simulations to summary statistics from localities sampled in the field, but: comparison of which localities and stepping stones? Identical stepping stones can be arranged so two localities fall in the same stepping stone, nearest or diagonal neighbours, or without contact. None is intrinsically correct, yet some choice must be made and this affects inference. We explore a Bayesian strategy for mapping field observations onto discrete stepping stones. We make Sundial, for projecting field data onto the plane, available. We generalize KSS over regular tilings of the plane. We show Bayesian averaging over the mapping between a continuous field area and discrete stepping stones improves the fit between KSS and isolation by distance expectations. We make Tiler Durden available for carrying out this Bayesian averaging. We describe a novel parameterization of KSS based on Wright's neighbourhood size, placing an upper bound on the geographic area represented by a stepping stone and make it available as m Vector. We generalize spatial coalescence recursions to continuous and discrete space cases and use these to numerically solve for KSS coalescence previously examined only using simulation. We thus provide applied and analytical resources for comparison of stepping stone simulations with field observations.

  7. Discrete Roughness Transition for Hypersonic Flight Vehicles

    NASA Technical Reports Server (NTRS)

    Berry, Scott A.; Horvath, Thomas J.

    2007-01-01

    The importance of discrete roughness and the correlations developed to predict the onset of boundary layer transition on hypersonic flight vehicles are discussed. The paper is organized by hypersonic vehicle applications characterized in a general sense by the boundary layer: slender with hypersonic conditions at the edge of the boundary layer, moderately blunt with supersonic, and blunt with subsonic. This paper is intended to be a review of recent discrete roughness transition work completed at NASA Langley Research Center in support of agency flight test programs. First, a review is provided of discrete roughness wind tunnel data and the resulting correlations that were developed. Then, results obtained from flight vehicles, in particular the recently flown Hyper-X and Shuttle missions, are discussed and compared to the ground-based correlations.

  8. Discrete energy bands in bulk semiconductors

    NASA Astrophysics Data System (ADS)

    Du, Maohua; Shi, Hongliang

    2015-03-01

    Bulk semiconductors typically have continuous valence and conduction bands. Discrete energy levels and bands have been sought after for various applications. For instance, discrete energy levels existing in semiconductor nanocrystals, or quantum does (QDs) have been proposed as a mechanism to suppress hot carrier thermalization and to enhance carrier multiplication in QD solar cells. Impurity bands in the band gap have been introduced for intermediate-band solar cells and for efficient visible light absorption and photocatalysis. In this talk, we show by first principles calculations that, in a multinary compound, a combination of large electronegativity difference between different cations (anions) and large nearest-neighbor distances in cation (anion) sublattices can lead to the splitting of the conduction (valence) band, resulting in several discrete and narrow energy bands separated by large energy gaps. We also discuss applications that may benefit from such electronic structure.

  9. Discrete Time Crystals: Rigidity, Criticality, and Realizations

    NASA Astrophysics Data System (ADS)

    Yao, N. Y.; Potter, A. C.; Potirniche, I.-D.; Vishwanath, A.

    2017-01-01

    Despite being forbidden in equilibrium, spontaneous breaking of time translation symmetry can occur in periodically driven, Floquet systems with discrete time-translation symmetry. The period of the resulting discrete time crystal is quantized to an integer multiple of the drive period, arising from a combination of collective synchronization and many body localization. Here, we consider a simple model for a one-dimensional discrete time crystal which explicitly reveals the rigidity of the emergent oscillations as the drive is varied. We numerically map out its phase diagram and compute the properties of the dynamical phase transition where the time crystal melts into a trivial Floquet insulator. Moreover, we demonstrate that the model can be realized with current experimental technologies and propose a blueprint based upon a one dimensional chain of trapped ions. Using experimental parameters (featuring long-range interactions), we identify the phase boundaries of the ion-time-crystal and propose a measurable signature of the symmetry breaking phase transition.

  10. Discrete stacking of aromatic oligoamide macrocycles.

    PubMed

    Wu, Xiangxiang; Liu, Rui; Sathyamoorthy, Bharathwaj; Yamato, Kazuhiro; Liang, Guoxing; Shen, Lin; Ma, Sufang; Sukumaran, Dinesh K; Szyperski, Thomas; Fang, Weihai; He, Lan; Chen, Xuebo; Gong, Bing

    2015-05-13

    Unlike the precise structural control typical of closed assemblies, curbing the stacking of disc- and ring-shaped molecules is quite challenging. Here we report the discrete stacking of rigid aromatic oligoamide macrocycles 1. With increasing concentration, the aggregation of 1 quickly plateaus, forming a discrete oligomer, as suggested by 1D (1)H, 2D nuclear Overhauser effect, and diffusion-ordered NMR spectroscopy. Quantum-chemical calculations indicate that the tetramer of 1 is the most stable among oligomeric stacks. X-ray crystallography revealed a tetrameric stack containing identical molecules adopting two different conformations. With a defined length and an inner pore capable of accommodating distinctly different guests, the tetramers of 1 densely pack into 2D layers. Besides being a rare system of conformation-regulated supramolecular oligomerization, the discrete stacks of 1, along with their higher-order assemblies, may offer new nanotechnological applications.

  11. Tree Ensembles on the Induced Discrete Space.

    PubMed

    Yildiz, Olcay Taner

    2016-05-01

    Decision trees are widely used predictive models in machine learning. Recently, K -tree is proposed, where the original discrete feature space is expanded by generating all orderings of values of k discrete attributes and these orderings are used as the new attributes in decision tree induction. Although K -tree performs significantly better than the proper one, their exponential time complexity can prohibit their use. In this brief, we propose K -forest, an extension of random forest, where a subset of features is selected randomly from the induced discrete space. Simulation results on 17 data sets show that the novel ensemble classifier has significantly lower error rate compared with the random forest based on the original feature space.

  12. Equivalent Hamiltonians with additional discrete states

    NASA Astrophysics Data System (ADS)

    Chinn, C. R.; Thaler, R. M.

    1991-01-01

    Given a particular Hamiltonian H, we present a method to generate a new Hamiltonian H~, which has the same discrete energy eigenvalues and the same continuum phase shifts as H, but which also has additional given discrete eigenstates. This method is used to generate a Hamiltonian h1, which gives rise to a complete orthonormal set of basis states, which contain a given set of biorthonormal discrete states, the continuum states of which are asymptotic to plane waves (have zero phase shifts). Such a set of states may be helpful in representing the medium modification of the Green's function due to the Pauli principle, as well as including Pauli exclusion effects into scattering calculations.

  13. Discrete Time Crystals: Rigidity, Criticality, and Realizations.

    PubMed

    Yao, N Y; Potter, A C; Potirniche, I-D; Vishwanath, A

    2017-01-20

    Despite being forbidden in equilibrium, spontaneous breaking of time translation symmetry can occur in periodically driven, Floquet systems with discrete time-translation symmetry. The period of the resulting discrete time crystal is quantized to an integer multiple of the drive period, arising from a combination of collective synchronization and many body localization. Here, we consider a simple model for a one-dimensional discrete time crystal which explicitly reveals the rigidity of the emergent oscillations as the drive is varied. We numerically map out its phase diagram and compute the properties of the dynamical phase transition where the time crystal melts into a trivial Floquet insulator. Moreover, we demonstrate that the model can be realized with current experimental technologies and propose a blueprint based upon a one dimensional chain of trapped ions. Using experimental parameters (featuring long-range interactions), we identify the phase boundaries of the ion-time-crystal and propose a measurable signature of the symmetry breaking phase transition.

  14. An algebra of discrete event processes

    NASA Technical Reports Server (NTRS)

    Heymann, Michael; Meyer, George

    1991-01-01

    This report deals with an algebraic framework for modeling and control of discrete event processes. The report consists of two parts. The first part is introductory, and consists of a tutorial survey of the theory of concurrency in the spirit of Hoare's CSP, and an examination of the suitability of such an algebraic framework for dealing with various aspects of discrete event control. To this end a new concurrency operator is introduced and it is shown how the resulting framework can be applied. It is further shown that a suitable theory that deals with the new concurrency operator must be developed. In the second part of the report the formal algebra of discrete event control is developed. At the present time the second part of the report is still an incomplete and occasionally tentative working paper.

  15. LAPS discretization and solution of plasma equilibrium

    NASA Astrophysics Data System (ADS)

    Missanelli, Maria; Delzanno, Gian Luca; Guo, Zehua; Srinivasan, Bhuvana; Tang, Xianzhu

    2011-10-01

    LAPS provides spectral element discretization for solving steady state plasma profiles. Our initial focus is on its implementation for two dimensional open magnetic field equilibria in linear and toroidal geometries. The linear geometry is an axisymmetric magnetic mirror with anisotropic pressure. The toroidal case is a tokamak scrape-off layer plasma. Structured grids are produced by the grid generation package in LAPS. The spectral element discretization uses modal bases over quadrilateral elements. A Newton-Krylov solver implemented with the Portable, Extensible Toolkits for Scientific Computing PETSc is applied to iteratively converge the solution. Care has been taken in the code design to separate the grid generation, spectral element discretization, and (non)linear solver from the user-specified equilibrium equations, so the LAPS infrastructure can be easily used for different applications. Work supported by DOE OFES.

  16. Optimal Learning Rules for Discrete Synapses

    PubMed Central

    Barrett, Adam B.; van Rossum, M. C. W.

    2008-01-01

    There is evidence that biological synapses have a limited number of discrete weight states. Memory storage with such synapses behaves quite differently from synapses with unbounded, continuous weights, as old memories are automatically overwritten by new memories. Consequently, there has been substantial discussion about how this affects learning and storage capacity. In this paper, we calculate the storage capacity of discrete, bounded synapses in terms of Shannon information. We use this to optimize the learning rules and investigate how the maximum information capacity depends on the number of synapses, the number of synaptic states, and the coding sparseness. Below a certain critical number of synapses per neuron (comparable to numbers found in biology), we find that storage is similar to unbounded, continuous synapses. Hence, discrete synapses do not necessarily have lower storage capacity. PMID:19043540

  17. Discrete-time Markovian stochastic Petri nets

    NASA Technical Reports Server (NTRS)

    Ciardo, Gianfranco

    1995-01-01

    We revisit and extend the original definition of discrete-time stochastic Petri nets, by allowing the firing times to have a 'defective discrete phase distribution'. We show that this formalism still corresponds to an underlying discrete-time Markov chain. The structure of the state for this process describes both the marking of the Petri net and the phase of the firing time for each transition, resulting in a large state space. We then modify the well-known power method to perform a transient analysis even when the state space is infinite, subject to the condition that only a finite number of states can be reached in a finite amount of time. Since the memory requirements might still be excessive, we suggest a bounding technique based on truncation.

  18. Discrete breathers in hexagonal dusty plasma lattices

    SciTech Connect

    Koukouloyannis, V.; Kourakis, I.

    2009-08-15

    The occurrence of single-site or multisite localized vibrational modes, also called discrete breathers, in two-dimensional hexagonal dusty plasma lattices is investigated. The system is described by a Klein-Gordon hexagonal lattice characterized by a negative coupling parameter epsilon in account of its inverse dispersive behavior. A theoretical analysis is performed in order to establish the possibility of existence of single as well as three-site discrete breathers in such systems. The study is complemented by a numerical investigation based on experimentally provided potential forms. This investigation shows that a dusty plasma lattice can support single-site discrete breathers, while three-site in phase breathers could exist if specific conditions, about the intergrain interaction strength, would hold. On the other hand, out of phase and vortex three-site breathers cannot be supported since they are highly unstable.

  19. Natural discretization in noncommutative field theory

    SciTech Connect

    Acatrinei, Ciprian Sorin

    2015-12-07

    A discretization scheme for field theory is developed, in which the space time coordinates are assumed to be operators forming a noncommutative algebra. Generic waves without rotational symmetry are studied in (2+1) - dimensional scalar field theory with Heisenberg-type noncommutativity. In the representation chosen, the radial coordinate is naturally rendered discrete. Nonlocality along this coordinate, induced by noncommutativity, accounts for the angular dependence of the fields. A complete solution and the interpretation of its nonlocal features are given. The exact form of standing and propagating waves on such a discrete space is found in terms of finite series. A precise correspondence is established between the degree of nonlocality and the angular momentum of a field configuration. At small distance no classical singularities appear, even at the location of the sources. At large radius one recovers the usual commutative/continuum behaviour.

  20. Insight from modelling discrete fractures using GEOCRACK

    SciTech Connect

    DuTeaux, Robert; Swenson, Daniel; Hardeman, Brian

    1996-01-24

    This work analyzes the behavior of a numerical geothermal reservoir simulation with flow only in discrete fractures. GEOCRACK is a 2-D finite element model developed at Kansas State University for the Hot Dry Rock (HDR) research at Los Alamos National Laboratory. Its numerical simulations couple the mechanics of discrete fracture behavior with the state of earth stress, fluid flow, and heat transfer. This coupled model could also be of value for modeling reinjection and other reservoir operating strategies for liquid dominated fractured reservoirs. Because fracture surfaces cool quickly by fluid convection, and heat does not conduct quickly from the interior of reservoir rock, modeling the injection of cold fluid into a fractured reservoir is better simulated by a model with discrete fractures. This work contains knowledge gained from HDR reservoir simulation and continues to develop the general concept of heat mining, reservoir optimization. and the sensitivity of simulation to the uncertainties of fracture spacing and dynamic flow dispersion.

  1. Natural discretization in noncommutative field theory

    NASA Astrophysics Data System (ADS)

    Acatrinei, Ciprian Sorin

    2015-12-01

    A discretization scheme for field theory is developed, in which the space time coordinates are assumed to be operators forming a noncommutative algebra. Generic waves without rotational symmetry are studied in (2+1) - dimensional scalar field theory with Heisenberg-type noncommutativity. In the representation chosen, the radial coordinate is naturally rendered discrete. Nonlocality along this coordinate, induced by noncommutativity, accounts for the angular dependence of the fields. A complete solution and the interpretation of its nonlocal features are given. The exact form of standing and propagating waves on such a discrete space is found in terms of finite series. A precise correspondence is established between the degree of nonlocality and the angular momentum of a field configuration. At small distance no classical singularities appear, even at the location of the sources. At large radius one recovers the usual commutative/continuum behaviour.

  2. Superheavy dark matter with discrete gauge symmetries

    NASA Astrophysics Data System (ADS)

    Hamaguchi, K.; Nomura, Yasunori; Yanagida, T.

    1998-11-01

    We show that there are discrete gauge symmetries which naturally protect heavy X particles from decaying into ordinary light particles in the supersymmetric standard model. This makes the proposal that superheavy X particles constitute part of the dark matter in the present universe very attractive. It is more interesting that there is a class of discrete gauge symmetries which naturally accommodates a long-lived unstable X particle. We find that in some discrete Z10 models, for example, a superheavy X particle has a lifetime of τX~=1011-1026 yr for a mass of MX~=1013-1014 GeV. This long lifetime is guaranteed by the absence of lower dimensional operators (of light particles) coupled to the X. We briefly discuss a possible explanation for the recently observed ultrahigh-energy cosmic ray events by the decay of this unstable X particle.

  3. Discrete - Continuous Duality of Protein Structure Space

    PubMed Central

    Sadreyev, Ruslan I.; Kim, Bong-Hyun; Grishin, Nick V.

    2012-01-01

    Summary Recently, the nature of protein structure space has been widely discussed in the literature. The traditional discrete view of protein universe as a set of separate folds has been criticized in the light of growing evidence that almost any arrangement of secondary structures is possible and the whole protein space can be traversed through a path of similar structures. Here we argue that the discrete and continuous descriptions are not mutually exclusive, but complementary: the space is largely discrete in evolutionary sense, but continuous geometrically when purely structural similarities are quantified. Evolutionary connections are mainly confined to separate structural prototypes corresponding to folds as islands of structural stability, with few remaining traceable links between the islands. However, for a geometric similarity measure, it is usually possible to find a reasonable cutoff that yields paths connecting any two structures through intermediates. PMID:19482467

  4. The ultimatum game: Discrete vs. continuous offers

    NASA Astrophysics Data System (ADS)

    Dishon-Berkovits, Miriam; Berkovits, Richard

    2014-09-01

    In many experimental setups in social-sciences, psychology and economy the subjects are requested to accept or dispense monetary compensation which is usually given in discrete units. Using computer and mathematical modeling we show that in the framework of studying the dynamics of acceptance of proposals in the ultimatum game, the long time dynamics of acceptance of offers in the game are completely different for discrete vs. continuous offers. For discrete values the dynamics follow an exponential behavior. However, for continuous offers the dynamics are described by a power-law. This is shown using an agent based computer simulation as well as by utilizing an analytical solution of a mean-field equation describing the model. These findings have implications to the design and interpretation of socio-economical experiments beyond the ultimatum game.

  5. Limit sets for the discrete spectrum of complex Jacobi matrices

    NASA Astrophysics Data System (ADS)

    Golinskii, L. B.; Egorova, I. E.

    2005-06-01

    The discrete spectrum of complex Jacobi matrices that are compact perturbations of the discrete Laplacian is studied. The precise stabilization rate (in the sense of order) of the matrix elements ensuring the finiteness of the discrete spectrum is found. An example of a Jacobi matrix with discrete spectrum having a unique limit point is constructed. These results are discrete analogues of Pavlov's well-known results on Schrödinger operators with complex potential on a half-axis.

  6. SPAR-H Step-by-Step Guidance

    SciTech Connect

    W. J. Galyean; A. M. Whaley; D. L. Kelly; R. L. Boring

    2011-05-01

    This guide provides step-by-step guidance on the use of the SPAR-H method for quantifying Human Failure Events (HFEs). This guide is intended to be used with the worksheets provided in: 'The SPAR-H Human Reliability Analysis Method,' NUREG/CR-6883, dated August 2005. Each step in the process of producing a Human Error Probability (HEP) is discussed. These steps are: Step-1, Categorizing the HFE as Diagnosis and/or Action; Step-2, Rate the Performance Shaping Factors; Step-3, Calculate PSF-Modified HEP; Step-4, Accounting for Dependence, and; Step-5, Minimum Value Cutoff. The discussions on dependence are extensive and include an appendix that describes insights obtained from the psychology literature.

  7. The discrete regime of flame propagation

    NASA Astrophysics Data System (ADS)

    Tang, Francois-David; Goroshin, Samuel; Higgins, Andrew

    The propagation of laminar dust flames in iron dust clouds was studied in a low-gravity envi-ronment on-board a parabolic flight aircraft. The elimination of buoyancy-induced convection and particle settling permitted measurements of fundamental combustion parameters such as the burning velocity and the flame quenching distance over a wide range of particle sizes and in different gaseous mixtures. The discrete regime of flame propagation was observed by substitut-ing nitrogen present in air with xenon, an inert gas with a significantly lower heat conductivity. Flame propagation in the discrete regime is controlled by the heat transfer between neighbor-ing particles, rather than by the particle burning rate used by traditional continuum models of heterogeneous flames. The propagation mechanism of discrete flames depends on the spa-tial distribution of particles, and thus such flames are strongly influenced by local fluctuations in the fuel concentration. Constant pressure laminar dust flames were observed inside 70 cm long, 5 cm diameter Pyrex tubes. Equally-spaced plate assemblies forming rectangular chan-nels were placed inside each tube to determine the quenching distance defined as the minimum channel width through which a flame can successfully propagate. High-speed video cameras were used to measure the flame speed and a fiber optic spectrometer was used to measure the flame temperature. Experimental results were compared with predictions obtained from a numerical model of a three-dimensional flame developed to capture both the discrete nature and the random distribution of particles in the flame. Though good qualitative agreement was obtained between model predictions and experimental observations, residual g-jitters and the short reduced-gravity periods prevented further investigations of propagation limits in the dis-crete regime. The full exploration of the discrete flame phenomenon would require high-quality, long duration reduced gravity environment

  8. Cortical Neural Computation by Discrete Results Hypothesis.

    PubMed

    Castejon, Carlos; Nuñez, Angel

    2016-01-01

    One of the most challenging problems we face in neuroscience is to understand how the cortex performs computations. There is increasing evidence that the power of the cortical processing is produced by populations of neurons forming dynamic neuronal ensembles. Theoretical proposals and multineuronal experimental studies have revealed that ensembles of neurons can form emergent functional units. However, how these ensembles are implicated in cortical computations is still a mystery. Although cell ensembles have been associated with brain rhythms, the functional interaction remains largely unclear. It is still unknown how spatially distributed neuronal activity can be temporally integrated to contribute to cortical computations. A theoretical explanation integrating spatial and temporal aspects of cortical processing is still lacking. In this Hypothesis and Theory article, we propose a new functional theoretical framework to explain the computational roles of these ensembles in cortical processing. We suggest that complex neural computations underlying cortical processing could be temporally discrete and that sensory information would need to be quantized to be computed by the cerebral cortex. Accordingly, we propose that cortical processing is produced by the computation of discrete spatio-temporal functional units that we have called "Discrete Results" (Discrete Results Hypothesis). This hypothesis represents a novel functional mechanism by which information processing is computed in the cortex. Furthermore, we propose that precise dynamic sequences of "Discrete Results" is the mechanism used by the cortex to extract, code, memorize and transmit neural information. The novel "Discrete Results" concept has the ability to match the spatial and temporal aspects of cortical processing. We discuss the possible neural underpinnings of these functional computational units and describe the empirical evidence supporting our hypothesis. We propose that fast-spiking (FS

  9. Cortical Neural Computation by Discrete Results Hypothesis

    PubMed Central

    Castejon, Carlos; Nuñez, Angel

    2016-01-01

    One of the most challenging problems we face in neuroscience is to understand how the cortex performs computations. There is increasing evidence that the power of the cortical processing is produced by populations of neurons forming dynamic neuronal ensembles. Theoretical proposals and multineuronal experimental studies have revealed that ensembles of neurons can form emergent functional units. However, how these ensembles are implicated in cortical computations is still a mystery. Although cell ensembles have been associated with brain rhythms, the functional interaction remains largely unclear. It is still unknown how spatially distributed neuronal activity can be temporally integrated to contribute to cortical computations. A theoretical explanation integrating spatial and temporal aspects of cortical processing is still lacking. In this Hypothesis and Theory article, we propose a new functional theoretical framework to explain the computational roles of these ensembles in cortical processing. We suggest that complex neural computations underlying cortical processing could be temporally discrete and that sensory information would need to be quantized to be computed by the cerebral cortex. Accordingly, we propose that cortical processing is produced by the computation of discrete spatio-temporal functional units that we have called “Discrete Results” (Discrete Results Hypothesis). This hypothesis represents a novel functional mechanism by which information processing is computed in the cortex. Furthermore, we propose that precise dynamic sequences of “Discrete Results” is the mechanism used by the cortex to extract, code, memorize and transmit neural information. The novel “Discrete Results” concept has the ability to match the spatial and temporal aspects of cortical processing. We discuss the possible neural underpinnings of these functional computational units and describe the empirical evidence supporting our hypothesis. We propose that fast

  10. Tracking vortices in superconductors: Extracting singularities from a discretized complex scalar field evolving in time

    DOE PAGES

    Phillips, Carolyn L.; Guo, Hanqi; Peterka, Tom; ...

    2016-02-19

    In type-II superconductors, the dynamics of magnetic flux vortices determine their transport properties. In the Ginzburg-Landau theory, vortices correspond to topological defects in the complex order parameter field. Earlier, we introduced a method for extracting vortices from the discretized complex order parameter field generated by a large-scale simulation of vortex matter. With this method, at a fixed time step, each vortex [simplistically, a one-dimensional (1D) curve in 3D space] can be represented as a connected graph extracted from the discretized field. Here we extend this method as a function of time as well. A vortex now corresponds to a 2Dmore » space-time sheet embedded in 4D space time that can be represented as a connected graph extracted from the discretized field over both space and time. Vortices that interact by merging or splitting correspond to disappearance and appearance of holes in the connected graph in the time direction. This method of tracking vortices, which makes no assumptions about the scale or behavior of the vortices, can track the vortices with a resolution as good as the discretization of the temporally evolving complex scalar field. In addition, even details of the trajectory between time steps can be reconstructed from the connected graph. With this form of vortex tracking, the details of vortex dynamics in a model of a superconducting materials can be understood in greater detail than previously possible.« less

  11. Tracking vortices in superconductors: Extracting singularities from a discretized complex scalar field evolving in time

    SciTech Connect

    Phillips, Carolyn L.; Guo, Hanqi; Peterka, Tom; Karpeyev, Dmitry; Glatz, Andreas

    2016-02-01

    In type-II superconductors, the dynamics of magnetic flux vortices determine their transport properties. In the Ginzburg-Landau theory, vortices correspond to topological defects in the complex order parameter field. Earlier, in Phillips et al. [Phys. Rev. E 91, 023311 (2015)], we introduced a method for extracting vortices from the discretized complex order parameter field generated by a large-scale simulation of vortex matter. With this method, at a fixed time step, each vortex [simplistically, a one-dimensional (1D) curve in 3D space] can be represented as a connected graph extracted from the discretized field. Here we extend this method as a function of time as well. A vortex now corresponds to a 2D space-time sheet embedded in 4D space time that can be represented as a connected graph extracted from the discretized field over both space and time. Vortices that interact by merging or splitting correspond to disappearance and appearance of holes in the connected graph in the time direction. This method of tracking vortices, which makes no assumptions about the scale or behavior of the vortices, can track the vortices with a resolution as good as the discretization of the temporally evolving complex scalar field. Additionally, even details of the trajectory between time steps can be reconstructed from the connected graph. With this form of vortex tracking, the details of vortex dynamics in a model of a superconducting materials can be understood in greater detail than previously possible.

  12. Tracking vortices in superconductors: Extracting singularities from a discretized complex scalar field evolving in time

    SciTech Connect

    Phillips, Carolyn L.; Guo, Hanqi; Peterka, Tom; Karpeyev, Dmitry; Glatz, Andreas

    2016-02-19

    In type-II superconductors, the dynamics of magnetic flux vortices determine their transport properties. In the Ginzburg-Landau theory, vortices correspond to topological defects in the complex order parameter field. Earlier, we introduced a method for extracting vortices from the discretized complex order parameter field generated by a large-scale simulation of vortex matter. With this method, at a fixed time step, each vortex [simplistically, a one-dimensional (1D) curve in 3D space] can be represented as a connected graph extracted from the discretized field. Here we extend this method as a function of time as well. A vortex now corresponds to a 2D space-time sheet embedded in 4D space time that can be represented as a connected graph extracted from the discretized field over both space and time. Vortices that interact by merging or splitting correspond to disappearance and appearance of holes in the connected graph in the time direction. This method of tracking vortices, which makes no assumptions about the scale or behavior of the vortices, can track the vortices with a resolution as good as the discretization of the temporally evolving complex scalar field. In addition, even details of the trajectory between time steps can be reconstructed from the connected graph. With this form of vortex tracking, the details of vortex dynamics in a model of a superconducting materials can be understood in greater detail than previously possible.

  13. Simulations of incompressible Navier Stokes equations on curved surfaces using discrete exterior calculus

    NASA Astrophysics Data System (ADS)

    Samtaney, Ravi; Mohamed, Mamdouh; Hirani, Anil

    2015-11-01

    We present examples of numerical solutions of incompressible flow on 2D curved domains. The Navier-Stokes equations are first rewritten using the exterior calculus notation, replacing vector calculus differential operators by the exterior derivative, Hodge star and wedge product operators. A conservative discretization of Navier-Stokes equations on simplicial meshes is developed based on discrete exterior calculus (DEC). The discretization is then carried out by substituting the corresponding discrete operators based on the DEC framework. By construction, the method is conservative in that both the discrete divergence and circulation are conserved up to machine precision. The relative error in kinetic energy for inviscid flow test cases converges in a second order fashion with both the mesh size and the time step. Numerical examples include Taylor vortices on a sphere, Stuart vortices on a sphere, and flow past a cylinder on domains with varying curvature. Supported by the KAUST Office of Competitive Research Funds under Award No. URF/1/1401-01.

  14. Limitations of discrete-time approaches to continuous-time contagion dynamics

    NASA Astrophysics Data System (ADS)

    Fennell, Peter G.; Melnik, Sergey; Gleeson, James P.

    2016-11-01

    Continuous-time Markov process models of contagions are widely studied, not least because of their utility in predicting the evolution of real-world contagions and in formulating control measures. It is often the case, however, that discrete-time approaches are employed to analyze such models or to simulate them numerically. In such cases, time is discretized into uniform steps and transition rates between states are replaced by transition probabilities. In this paper, we illustrate potential limitations to this approach. We show how discretizing time leads to a restriction on the values of the model parameters that can accurately be studied. We examine numerical simulation schemes employed in the literature, showing how synchronous-type updating schemes can bias discrete-time formalisms when compared against continuous-time formalisms. Event-based simulations, such as the Gillespie algorithm, are proposed as optimal simulation schemes both in terms of replicating the continuous-time process and computational speed. Finally, we show how discretizing time can affect the value of the epidemic threshold for large values of the infection rate and the recovery rate, even if the ratio between the former and the latter is small.

  15. A multi-time step integration algorithm for diffusion problems using a dual method

    NASA Astrophysics Data System (ADS)

    Beneš, Michal

    2017-07-01

    Nonstationary partial differential equations are numerically solved by discretizing in space and then integrating over time using discrete solvers. In this contribution we present a new multi-time stepping strategy for diffusion problems. The computational domain is divided into a set of smaller subdomains that may be integrated sequentially with its own time steps and different methods. The continuity condition at the interface is ensured using a dual Schur formulation. Decoupled problems may be less expansive and sequential computation may be less memory intensive. The numerical convergence analysis of the proposed algorithm is illustrated by means of split degree-of-freedom problem, the analytical solution of which is known.

  16. Optical Planar Discrete Fourier and Wavelet Transforms

    NASA Astrophysics Data System (ADS)

    Cincotti, Gabriella; Moreolo, Michela Svaluto; Neri, Alessandro

    2007-10-01

    We present all-optical architectures to perform discrete wavelet transform (DWT), wavelet packet (WP) decomposition and discrete Fourier transform (DFT) using planar lightwave circuits (PLC) technology. Any compact-support wavelet filter can be implemented as an optical planar two-port lattice-form device, and different subband filtering schemes are possible to denoise, or multiplex optical signals. We consider both parallel and serial input cases. We design a multiport decoder/decoder that is able to generate/process optical codes simultaneously and a flexible logarithmic wavelength multiplexer, with flat top profile and reduced crosstalk.

  17. Discrete Gabor Filters For Binocular Disparity Measurement

    NASA Technical Reports Server (NTRS)

    Weiman, Carl F. R.

    1995-01-01

    Discrete Gabor filters proposed for use in determining binocular disparity - difference between positions of same feature or object depicted in stereoscopic images produced by two side-by-side cameras aimed in parallel. Magnitude of binocular disparity used to estimate distance from cameras to feature or object. In one potential application, cameras charge-coupled-device video cameras in robotic vision system, and binocular disparities and distance estimates used as control inputs - for example, to control approaches to objects manipulated or to maintain safe distances from obstacles. Binocular disparities determined from phases of discretized Gabor transforms.

  18. Hybrid Discrete-Continuous Markov Decision Processes

    NASA Technical Reports Server (NTRS)

    Feng, Zhengzhu; Dearden, Richard; Meuleau, Nicholas; Washington, Rich

    2003-01-01

    This paper proposes a Markov decision process (MDP) model that features both discrete and continuous state variables. We extend previous work by Boyan and Littman on the mono-dimensional time-dependent MDP to multiple dimensions. We present the principle of lazy discretization, and piecewise constant and linear approximations of the model. Having to deal with several continuous dimensions raises several new problems that require new solutions. In the (piecewise) linear case, we use techniques from partially- observable MDPs (POMDPS) to represent value functions as sets of linear functions attached to different partitions of the state space.

  19. Engineering discrete stacks of aromatic molecules.

    PubMed

    Klosterman, Jeremy K; Yamauchi, Yoshihiro; Fujita, Makoto

    2009-06-01

    Intrigued by transannular interactions occurring in stacked aromatic molecules, chemists have long endeavored to engineer discrete stacks of specific lengths and orientation. The maturation of self-assembly methodologies has shifted the focus away from utilizing covalent scaffolds to harnessing non-covalent interactions such as ionic interactions, hydrogen bonds, metal-ligand interactions, and aromatic interactions. Aromatic molecules often assemble into ill-defined, infinite aggregates and thus multiple self-assembly techniques must be combined to achieve the desired stack size and conformations. This critical review briefly highlights covalent scaffolds of stack aromatics before focusing on modern self-assembly based strategies for engineering discrete stacks of aromatic molecules (149 references).

  20. Synchronization Of Parallel Discrete Event Simulations

    NASA Technical Reports Server (NTRS)

    Steinman, Jeffrey S.

    1992-01-01

    Adaptive, parallel, discrete-event-simulation-synchronization algorithm, Breathing Time Buckets, developed in Synchronous Parallel Environment for Emulation and Discrete Event Simulation (SPEEDES) operating system. Algorithm allows parallel simulations to process events optimistically in fluctuating time cycles that naturally adapt while simulation in progress. Combines best of optimistic and conservative synchronization strategies while avoiding major disadvantages. Algorithm processes events optimistically in time cycles adapting while simulation in progress. Well suited for modeling communication networks, for large-scale war games, for simulated flights of aircraft, for simulations of computer equipment, for mathematical modeling, for interactive engineering simulations, and for depictions of flows of information.

  1. Continuum modeling of the mechanical and thermal behavior of discrete large structures

    NASA Technical Reports Server (NTRS)

    Nayfeh, A. H.; Hefzy, M. S.

    1980-01-01

    In the present paper we introduce a rather straightforward construction procedure in order to derive continuum equivalence of discrete truss-like repetitive structures. Once the actual structure is specified, the construction procedure can be outlined by the following three steps: (a) all sets of parallel members are identified, (b) unidirectional 'effective continuum' properties are derived for each of these sets and (c) orthogonal transformations are finally used to determine the contribution of each set to the 'overall effective continuum' properties of the structure. Here the properties includes mechanical (stiffnesses), thermal (coefficients of thermal expansions) and material densities. Once expanded descriptions of the steps (b) and (c) are done, the construction procedure will be applied to a wide variety of discrete structures and the results will be compared with those of other existing methods.

  2. Stair-stepped Mound

    NASA Technical Reports Server (NTRS)

    2003-01-01

    MGS MOC Release No. MOC2-429, 22 July 2003

    This April 2003 Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a stair-stepped mound of sedimentary rock (right of center) on the floor of a large impact crater in western Arabia Terra near 11.0oN, 4.4oW. Sedimentary rock outcrops are common in the craters of this region. The repeated thickness and uniformity of the layers that make up this mound suggest that their depositional environment was one in which cyclic or episodic events occurred over some period of time. The sediments might have been deposited in a lake, or they may have settled directly out of the atmosphere. Most of the layered material was later eroded away, leaving this circular mound and the other nearby mesas and knobs. The image is illuminated by sunlight from the lower left.

  3. Sequential design of discrete linear quadratic regulators via optimal root-locus techniques

    NASA Technical Reports Server (NTRS)

    Shieh, Leang S.; Yates, Robert E.; Ganesan, Sekar

    1989-01-01

    A sequential method employing classical root-locus techniques has been developed in order to determine the quadratic weighting matrices and discrete linear quadratic regulators of multivariable control systems. At each recursive step, an intermediate unity rank state-weighting matrix that contains some invariant eigenvectors of that open-loop matrix is assigned, and an intermediate characteristic equation of the closed-loop system containing the invariant eigenvalues is created.

  4. Fabrication of Discrete Nanosized Cobalt Particles Encapsulated Inside Single-Walled Carbon Nanotubes

    SciTech Connect

    Zoican Loebick, C.; Majewska, M; Ren, F; Haller, G; Pfefferle, L

    2010-01-01

    Single-walled carbon nanotubes (SWNT) with encapsulated nanosized cobalt particles have been synthesized by a facile and scalable method. In this approach, SWNT were filled with a cobalt acetylacetonate solution in dichloromethane by ultrasonication. In a second step, exposure to hydrogen at different temperatures released discrete cobalt particles of controllable size inside the SWNT cavity. The SWNT-Co particles systems were characterized by transmission electron microscopy, X-ray absorption spectroscopy, Raman spectroscopy, and thermal gravimetric analysis.

  5. Sequential design of discrete linear quadratic regulators via optimal root-locus techniques

    NASA Technical Reports Server (NTRS)

    Shieh, Leang S.; Yates, Robert E.; Ganesan, Sekar

    1989-01-01

    A sequential method employing classical root-locus techniques has been developed in order to determine the quadratic weighting matrices and discrete linear quadratic regulators of multivariable control systems. At each recursive step, an intermediate unity rank state-weighting matrix that contains some invariant eigenvectors of that open-loop matrix is assigned, and an intermediate characteristic equation of the closed-loop system containing the invariant eigenvalues is created.

  6. Teaching Discrete Mathematics with Graphing Calculators.

    ERIC Educational Resources Information Center

    Masat, Francis E.

    Graphing calculator use is often thought of in terms of pre-calculus or continuous topics in mathematics. This paper contains examples and activities that demonstrate useful, interesting, and easy ways to use a graphing calculator with discrete topics. Examples are given for each of the following topics: functions, mathematical induction and…

  7. Electrolytic plating apparatus for discrete microsized particles

    DOEpatents

    Mayer, Anton

    1976-11-30

    Method and apparatus are disclosed for electrolytically producing very uniform coatings of a desired material on discrete microsized particles. Agglomeration or bridging of the particles during the deposition process is prevented by imparting a sufficiently random motion to the particles that they are not in contact with a powered cathode for a time sufficient for such to occur.

  8. Electroless plating apparatus for discrete microsized particles

    DOEpatents

    Mayer, Anton

    1978-01-01

    Method and apparatus are disclosed for producing very uniform coatings of a desired material on discrete microsized particles by electroless techniques. Agglomeration or bridging of the particles during the deposition process is prevented by imparting a sufficiently random motion to the particles that they are not in contact with each other for a time sufficient for such to occur.

  9. Failure diagnosis using discrete event models

    SciTech Connect

    Sampath, M.; Sengupta, R.; Lafortune, S.; Teneketzis, D.; Sinnamohideen, K.

    1994-12-31

    We propose a Discrete Event Systems (DES) approach to the failure diagnosis problem. We present a methodology for modeling physical systems in a DES framework. We discuss the notion of diagnosability and present the construction procedure of the diagnoser. Finally, we illustrate our approach using a Heating, Ventilation and Air Conditioning (HVAC) system.

  10. All-optical discrete vortex switch

    SciTech Connect

    Desyatnikov, Anton S.; Dennis, Mark R.; Ferrando, Albert

    2011-06-15

    We introduce discrete vortex solitons and vortex breathers in circular arrays of nonlinear waveguides. The simplest vortex breather in a four-waveguide coupler is a nonlinear dynamic state changing its topological charge between +1 and -1 periodically during propagation. We find the stability domain for this solution and suggest an all-optical vortex switching scheme.

  11. Discrete Gust Model for Launch Vehicle Assessments

    NASA Technical Reports Server (NTRS)

    Leahy, Frank B.

    2008-01-01

    Analysis of spacecraft vehicle responses to atmospheric wind gusts during flight is important in the establishment of vehicle design structural requirements and operational capability. Typically, wind gust models can be either a spectral type determined by a random process having a wide range of wavelengths, or a discrete type having a single gust of predetermined magnitude and shape. Classical discrete models used by NASA during the Apollo and Space Shuttle Programs included a 9 m/sec quasi-square-wave gust with variable wavelength from 60 to 300 m. A later study derived discrete gust from a military specification (MIL-SPEC) document that used a "1-cosine" shape. The MIL-SPEC document contains a curve of non-dimensional gust magnitude as a function of non-dimensional gust half-wavelength based on the Dryden spectral model, but fails to list the equation necessary to reproduce the curve. Therefore, previous studies could only estimate a value of gust magnitude from the curve, or attempt to fit a function to it. This paper presents the development of the MIL-SPEC curve, and provides the necessary information to calculate discrete gust magnitudes as a function of both gust half-wavelength and the desired probability level of exceeding a specified gust magnitude.

  12. Discrete events as units of perceived time.

    PubMed

    Liverence, Brandon M; Scholl, Brian J

    2012-06-01

    In visual images, we perceive both space (as a continuous visual medium) and objects (that inhabit space). Similarly, in dynamic visual experience, we perceive both continuous time and discrete events. What is the relationship between these units of experience? The most intuitive answer may be similar to the spatial case: time is perceived as an underlying medium, which is later segmented into discrete event representations. Here we explore the opposite possibility--that our subjective experience of time itself can be influenced by how durations are temporally segmented, beyond more general effects of change and complexity. We show that the way in which a continuous dynamic display is segmented into discrete units (via a path shuffling manipulation) greatly influences duration judgments, independent of psychophysical factors previously implicated in time perception, such as overall stimulus energy, attention and predictability. It seems that we may use the passage of discrete events--and the boundaries between them--in our subjective experience as part of the raw material for inferring the strength of the underlying "current" of time.

  13. Kinematics of foldable discrete space cranes

    NASA Technical Reports Server (NTRS)

    Nayfeh, A. H.

    1985-01-01

    Exact kinematic description of a NASA proposed prototype foldable-deployable discrete space crane are presented. A computer program is developed which maps the geometry of the crane once controlling parameters are specified. The program uses a building block type approach in which it calculates the local coordinates of each repeating cell and then combines them with respect to a global coordinates system.

  14. The Discrete Site Sticky Wall Model.

    DTIC Science & Technology

    1986-05-27

    TECHNICAL REPORT #23 THE DISCRETE SITE STICKY WALL tMDEL by J.P. Badiali Laboratoire Propre No 15 de CNRS Physique des Liquides et Electrochimie Tour 22, 5e...Liquides et Electrochimie NTIS CRA&I DTIC TAB 5 Tour 22, 5e Etage, 4 Place Jussieu U’annou;.ced . J ’ tificatlo rn

  15. Discrete control of resonant wave energy devices.

    PubMed

    Clément, A H; Babarit, A

    2012-01-28

    Aiming at amplifying the energy productive motion of wave energy converters (WECs) in response to irregular sea waves, the strategies of discrete control presented here feature some major advantages over continuous control, which is known to require, for optimal operation, a bidirectional power take-off able to re-inject energy into the WEC system during parts of the oscillation cycles. Three different discrete control strategies are described: latching control, declutching control and the combination of both, which we term latched-operating-declutched control. It is shown that any of these methods can be applied with great benefit, not only to mono-resonant WEC oscillators, but also to bi-resonant and multi-resonant systems. For some of these applications, it is shown how these three discrete control strategies can be optimally defined, either by analytical solution for regular waves, or numerically, by applying the optimal command theory in irregular waves. Applied to a model of a seven degree-of-freedom system (the SEAREV WEC) to estimate its annual production on several production sites, the most efficient of these discrete control strategies was shown to double the energy production, regardless of the resource level of the site, which may be considered as a real breakthrough, rather than a marginal improvement.

  16. Discrete Event Simulation of Distributed Team Communication

    DTIC Science & Technology

    2012-03-22

    executable system architecture approach to discrete events system modeling using sysml in conjunction with colored petri net . In Systems Conference, 2008 2nd...operators. Mitchell found that IMPRINT predictions of communication times and frequencies correlated with recorded communications amongst a platoon of

  17. Discrete wavelength-locked external cavity laser

    NASA Technical Reports Server (NTRS)

    Pilgrim, Jeffrey S. (Inventor); Silver, Joel A. (Inventor)

    2005-01-01

    An external cavity laser (and method of generating laser light) comprising: a laser light source; means for collimating light output by the laser light source; a diffraction grating receiving collimated light; a cavity feedback mirror reflecting light received from the diffraction grating back to the diffraction grating; and means for reliably tuning the external cavity laser to discrete wavelengths.

  18. Web-Based Implementation of Discrete Mathematics

    ERIC Educational Resources Information Center

    Love, Tanzy; Keinert, Fritz; Shelley, Mack

    2006-01-01

    The Department of Mathematics at Iowa State University teaches a freshman-level Discrete Mathematics course with total enrollment of about 1,800 students per year. The traditional format includes large lectures, with about 150 students each, taught by faculty and temporary instructors in two class sessions per week and recitation sections, with…

  19. A deterministic discrete ordinates transport proxy application

    SciTech Connect

    2014-06-03

    Kripke is a simple 3D deterministic discrete ordinates (Sn) particle transport code that maintains the computational load and communications pattern of a real transport code. It is intended to be a research tool to explore different data layouts, new programming paradigms and computer architectures.

  20. Neutrino mass and mixing with discrete symmetry.

    PubMed

    King, Stephen F; Luhn, Christoph

    2013-05-01

    This is a review paper about neutrino mass and mixing and flavour model building strategies based on discrete family symmetry. After a pedagogical introduction and overview of the whole of neutrino physics, we focus on the PMNS mixing matrix and the latest global fits following the Daya Bay and RENO experiments which measure the reactor angle. We then describe the simple bimaximal, tri-bimaximal and golden ratio patterns of lepton mixing and the deviations required for a non-zero reactor angle, with solar or atmospheric mixing sum rules resulting from charged lepton corrections or residual trimaximal mixing. The different types of see-saw mechanism are then reviewed as well as the sequential dominance mechanism. We then give a mini-review of finite group theory, which may be used as a discrete family symmetry broken by flavons either completely, or with different subgroups preserved in the neutrino and charged lepton sectors. These two approaches are then reviewed in detail in separate chapters including mechanisms for flavon vacuum alignment and different model building strategies that have been proposed to generate the reactor angle. We then briefly review grand unified theories (GUTs) and how they may be combined with discrete family symmetry to describe all quark and lepton masses and mixing. Finally, we discuss three model examples which combine an SU(5) GUT with the discrete family symmetries A₄, S₄ and Δ(96).

  1. Fast Mix Table Construction for Material Discretization

    SciTech Connect

    Johnson, Seth R

    2013-01-01

    An effective hybrid Monte Carlo--deterministic implementation typically requires the approximation of a continuous geometry description with a discretized piecewise-constant material field. The inherent geometry discretization error can be reduced somewhat by using material mixing, where multiple materials inside a discrete mesh voxel are homogenized. Material mixing requires the construction of a ``mix table,'' which stores the volume fractions in every mixture so that multiple voxels with similar compositions can reference the same mixture. Mix table construction is a potentially expensive serial operation for large problems with many materials and voxels. We formulate an efficient algorithm to construct a sparse mix table in $O(\\text{number of voxels}\\times \\log \\text{number of mixtures})$ time. The new algorithm is implemented in ADVANTG and used to discretize continuous geometries onto a structured Cartesian grid. When applied to an end-of-life MCNP model of the High Flux Isotope Reactor with 270 distinct materials, the new method improves the material mixing time by a factor of 100 compared to a naive mix table implementation.

  2. Fast mix table construction for material discretization

    SciTech Connect

    Johnson, S. R.

    2013-07-01

    An effective hybrid Monte Carlo-deterministic implementation typically requires the approximation of a continuous geometry description with a discretized piecewise-constant material field. The inherent geometry discretization error can be reduced somewhat by using material mixing, where multiple materials inside a discrete mesh voxel are homogenized. Material mixing requires the construction of a 'mix table,' which stores the volume fractions in every mixture so that multiple voxels with similar compositions can reference the same mixture. Mix table construction is a potentially expensive serial operation for large problems with many materials and voxels. We formulate an efficient algorithm to construct a sparse mix table in O(number of voxels x log number of mixtures) time. The new algorithm is implemented in ADVANTG and used to discretize continuous geometries onto a structured Cartesian grid. When applied to an end-of-life MCNP model of the High Flux Isotope Reactor with 270 distinct materials, the new method improves the material mixing time by a factor of 100 compared to a naive mix table implementation. (authors)

  3. 5 CFR 572.102 - Agency discretion.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and transportation or interview expenses in filling any position, the agency should consider such factors as availability of funds as well as the desirability of conducting interviews for a particular job... TRANSPORTATION EXPENSES; NEW APPOINTEES AND INTERVIEWS § 572.102 Agency discretion. Payment of travel...

  4. Discrete Mathematics Course Supported by CAS MATHEMATICA

    ERIC Educational Resources Information Center

    Ivanov, O. A.; Ivanova, V. V.; Saltan, A. A.

    2017-01-01

    In this paper, we discuss examples of assignments for a course in discrete mathematics for undergraduate students majoring in business informatics. We consider several problems with computer-based solutions and discuss general strategies for using computers in teaching mathematics and its applications. In order to evaluate the effectiveness of our…

  5. Stable discrete representation of relativistically drifting plasmas

    NASA Astrophysics Data System (ADS)

    Kirchen, M.; Lehe, R.; Godfrey, B. B.; Dornmair, I.; Jalas, S.; Peters, K.; Vay, J.-L.; Maier, A. R.

    2016-10-01

    Representing the electrodynamics of relativistically drifting particle ensembles in discrete, co-propagating Galilean coordinates enables the derivation of a Particle-In-Cell algorithm that is intrinsically free of the numerical Cherenkov instability for plasmas flowing at a uniform velocity. Application of the method is shown by modeling plasma accelerators in a Lorentz-transformed optimal frame of reference.

  6. Discrete Events as Units of Perceived Time

    ERIC Educational Resources Information Center

    Liverence, Brandon M.; Scholl, Brian J.

    2012-01-01

    In visual images, we perceive both space (as a continuous visual medium) and objects (that inhabit space). Similarly, in dynamic visual experience, we perceive both continuous time and discrete events. What is the relationship between these units of experience? The most intuitive answer may be similar to the spatial case: time is perceived as an…

  7. Applied Behavior Analysis: Beyond Discrete Trial Teaching

    ERIC Educational Resources Information Center

    Steege, Mark W.; Mace, F. Charles; Perry, Lora; Longenecker, Harold

    2007-01-01

    We discuss the problem of autism-specific special education programs representing themselves as Applied Behavior Analysis (ABA) programs when the only ABA intervention employed is Discrete Trial Teaching (DTT), and often for limited portions of the school day. Although DTT has many advantages to recommend its use, it is not well suited to teach…

  8. Attractors for discrete periodic dynamical systems

    Treesearch

    John E. Franke; James F. Selgrade

    2003-01-01

    A mathematical framework is introduced to study attractors of discrete, nonautonomous dynamical systems which depend periodically on time. A structure theorem for such attractors is established which says that the attractor of a time-periodic dynamical system is the unin of attractors of appropriate autonomous maps. If the nonautonomous system is a perturbation of an...

  9. Conjugacy classes in discrete Heisenberg groups

    SciTech Connect

    Budylin, R Ya

    2014-08-01

    We study an extension of a discrete Heisenberg group coming from the theory of loop groups and find invariants of conjugacy classes in this group. In some cases, including the case of the integer Heisenberg group, we make these invariants more explicit. Bibliography: 4 titles.

  10. Discrete Events as Units of Perceived Time

    ERIC Educational Resources Information Center

    Liverence, Brandon M.; Scholl, Brian J.

    2012-01-01

    In visual images, we perceive both space (as a continuous visual medium) and objects (that inhabit space). Similarly, in dynamic visual experience, we perceive both continuous time and discrete events. What is the relationship between these units of experience? The most intuitive answer may be similar to the spatial case: time is perceived as an…

  11. Applied Behavior Analysis: Beyond Discrete Trial Teaching

    ERIC Educational Resources Information Center

    Steege, Mark W.; Mace, F. Charles; Perry, Lora; Longenecker, Harold

    2007-01-01

    We discuss the problem of autism-specific special education programs representing themselves as Applied Behavior Analysis (ABA) programs when the only ABA intervention employed is Discrete Trial Teaching (DTT), and often for limited portions of the school day. Although DTT has many advantages to recommend its use, it is not well suited to teach…

  12. Dimensionality Problem in Discrete Discriminant Analysis

    NASA Astrophysics Data System (ADS)

    Ferreira, Ana Sousa

    2011-09-01

    A high dimensional problem is very often in Discrete Discriminant Analysis (DDA) due to the fact that the number of parameters estimated in DDA models is very frequently too large. Then, the main problem is sparseness, in which some of the multinomial cells may have no data in the training sets (for one or several classes). Furthermore, there aren't truly reliable methods for selecting the most discrete discriminative features and often we deal with small sample sizes with classes not well separated. This dimensional DDA problem is often known as the "curse of dimensionality". In this context, a combining models approach seems to be promising since it is known that different DDA models perform differently on different subjects. This approach currently appears in an increasing number of papers aiming to obtain more robust and stable models. Thus, in discrete problems we propose new forms of modeling the conditional probability functions based on linear combinations of reference models (e.g. the Full Multinomial Model (FMM) and the First-order Independence Model (FOIM)). Recently, since class separability is another fundamental problem in discrete supervised problems we have focused in exploring measures for analyzing class separability. We investigate the performance of the present approaches on real and simulated data.

  13. A Note on Discrete Mathematics and Calculus.

    ERIC Educational Resources Information Center

    O'Reilly, Thomas J.

    1987-01-01

    Much of the current literature on the topic of discrete mathematics and calculus during the first two years of an undergraduate mathematics curriculum is cited. A relationship between the recursive integration formulas and recursively defined polynomials is described. A Pascal program is included. (Author/RH)

  14. Geometric Representations for Discrete Fourier Transforms

    NASA Technical Reports Server (NTRS)

    Cambell, C. W.

    1986-01-01

    Simple geometric representations show symmetry and periodicity of discrete Fourier transforms (DFT's). Help in visualizing requirements for storing and manipulating transform value in computations. Representations useful in any number of dimensions, but particularly in one-, two-, and three-dimensional cases often encountered in practice.

  15. Discrete Mathematics and the Secondary Mathematics Curriculum.

    ERIC Educational Resources Information Center

    Dossey, John

    Discrete mathematics, the mathematics of decision making for finite settings, is a topic of great interest in mathematics education at all levels. Attention is being focused on resolving the diversity of opinion concerning the exact nature of the subject, what content the curriculum should contain, who should study that material, and how that…

  16. Analysis hierarchical model for discrete event systems

    NASA Astrophysics Data System (ADS)

    Ciortea, E. M.

    2015-11-01

    The This paper presents the hierarchical model based on discrete event network for robotic systems. Based on the hierarchical approach, Petri network is analysed as a network of the highest conceptual level and the lowest level of local control. For modelling and control of complex robotic systems using extended Petri nets. Such a system is structured, controlled and analysed in this paper by using Visual Object Net ++ package that is relatively simple and easy to use, and the results are shown as representations easy to interpret. The hierarchical structure of the robotic system is implemented on computers analysed using specialized programs. Implementation of hierarchical model discrete event systems, as a real-time operating system on a computer network connected via a serial bus is possible, where each computer is dedicated to local and Petri model of a subsystem global robotic system. Since Petri models are simplified to apply general computers, analysis, modelling, complex manufacturing systems control can be achieved using Petri nets. Discrete event systems is a pragmatic tool for modelling industrial systems. For system modelling using Petri nets because we have our system where discrete event. To highlight the auxiliary time Petri model using transport stream divided into hierarchical levels and sections are analysed successively. Proposed robotic system simulation using timed Petri, offers the opportunity to view the robotic time. Application of goods or robotic and transmission times obtained by measuring spot is obtained graphics showing the average time for transport activity, using the parameters sets of finished products. individually.

  17. Geometry of Discrete-Time Spin Systems

    NASA Astrophysics Data System (ADS)

    McLachlan, Robert I.; Modin, Klas; Verdier, Olivier

    2016-10-01

    Classical Hamiltonian spin systems are continuous dynamical systems on the symplectic phase space (S^2)^n. In this paper, we investigate the underlying geometry of a time discretization scheme for classical Hamiltonian spin systems called the spherical midpoint method. As it turns out, this method displays a range of interesting geometrical features that yield insights and sets out general strategies for geometric time discretizations of Hamiltonian systems on non-canonical symplectic manifolds. In particular, our study provides two new, completely geometric proofs that the discrete-time spin systems obtained by the spherical midpoint method preserve symplecticity. The study follows two paths. First, we introduce an extended version of the Hopf fibration to show that the spherical midpoint method can be seen as originating from the classical midpoint method on T^*{R}^{2n} for a collective Hamiltonian. Symplecticity is then a direct, geometric consequence. Second, we propose a new discretization scheme on Riemannian manifolds called the Riemannian midpoint method. We determine its properties with respect to isometries and Riemannian submersions, and, as a special case, we show that the spherical midpoint method is of this type for a non-Euclidean metric. In combination with Kähler geometry, this provides another geometric proof of symplecticity.

  18. Green Schools Energy Project: A Step-by-Step Manual.

    ERIC Educational Resources Information Center

    Quigley, Gwen

    This publication contains a step-by-step guide for implementing an energy-saving project in local school districts: the installation of newer, more energy-efficient "T-8" fluorescent tube lights in place of "T-12" lights. Eleven steps are explained in detail: (1) find out what kind of lights the school district currently uses;…

  19. Temporal evolution of step-edge fluctuations under electromigration conditions

    NASA Astrophysics Data System (ADS)

    Rous, P. J.; Bole, T. W.

    2007-09-01

    The temporal evolution of step-edge fluctuations under electromigration conditions is analyzed using a continuum Langevin model. If the electromigration driving force acts in the step up or down direction, and step-edge diffusion is the dominant mass-transport mechanism, we find that significant deviations from the usual t1/4 scaling of the terrace-width correlation function occurs for a critical time τ which is dependent upon the three energy scales in the problem: kBT , the step stiffness, γ , and the bias associated with adatom hopping under the influence of an electromigration force, ±ΔU . For (t<τ) , the correlation function evolves as a superposition of t1/4 and t3/4 power laws. For t⩾τ a closed form expression can be derived. This behavior is confirmed by a Monte Carlo simulation using a discrete model of the step dynamics. It is proposed that the magnitude of the electromigration force acting upon an atom at a step edge can be estimated by a careful analysis of the statistical properties of step-edge fluctuations on the appropriate time scale.

  20. Stability of discrete time recurrent neural networks and nonlinear optimization problems.

    PubMed

    Singh, Jayant; Barabanov, Nikita

    2016-02-01

    We consider the method of Reduction of Dissipativity Domain to prove global Lyapunov stability of Discrete Time Recurrent Neural Networks. The standard and advanced criteria for Absolute Stability of these essentially nonlinear systems produce rather weak results. The method mentioned above is proved to be more powerful. It involves a multi-step procedure with maximization of special nonconvex functions over polytopes on every step. We derive conditions which guarantee an existence of at most one point of local maximum for such functions over every hyperplane. This nontrivial result is valid for wide range of neuron transfer functions. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Continuous versus discrete for interacting carbon nanostructures

    NASA Astrophysics Data System (ADS)

    Hilder, Tamsyn A.; Hill, James M.

    2007-04-01

    Intermolecular forces between two interacting nanostructures can be obtained by either summing over all the individual atomic interactions or by using a continuum or continuous approach, where the number of atoms situated at discrete locations is averaged over the surface of each molecule. This paper aims to undertake a limited comparison of the continuum approach, the discrete atom-atom formulation and a hybrid discrete-continuum formulation for a range of molecular interactions involving a carbon nanotube, including interactions with another carbon nanotube and the fullerenes C60, C70 and C80. In the hybrid approach only one of the interacting molecules is discretized and the other is considered to be continuous. The hybrid discrete-continuum formulation would enable non-regular shaped molecules to be described, particularly useful for drug delivery systems which employ carbon nanotubes as carriers. The present investigation is important to obtain a rough estimate of the anticipated percentage errors which may occur between the various approaches in any specific application. Although our investigation is by no means comprehensive, overall we show that typically the interaction energies for these three approaches differ on average by at most 10% and the forces by 5%, with the exception of the C80 fullerene. For the C80 fullerene, while the intermolecular forces and the suction energies are in reasonable overall agreement, the point-wise energies can be significantly different. This may in part be due to differences in modelling the geometry of the C80 fullerene, but also the suction energies involve integrals of the energy, and therefore any errors or discrepancies in the point-wise energy tend to be smoothed out to give reasonable overall agreement for the former quantities.

  2. The Stepping Stone Approach

    NASA Astrophysics Data System (ADS)

    Brumfitt, A.

    Education is a profession in its own right. It has its own parameters, passions and language. Having the responsibility both of educare and educere, education has a focus of delivering specific factual knowledge whilst drawing out the creative mind. Space Science is a special vehicle having the properties of both educare and educere. It has a magic and wonder that touches the very essence of an individual and his place in time and space; it offers the "wow" factor that all teachers strive for. Space Science is the wrapping paper for other elements in the curriculum, e.g. cross-curricula and skill-based activities, such as language development, creativity, etc. as well as the pure sciences which comprise of engineering, physics and other natural sciences from astronomy to chemistry to biology. Each of these spheres of influence are relevant from kindergarten to undergraduate studies and complement, and in addition support informal education in museums, science centers and the world of e-learning. ESA Science Education has devised the "Stepping Stone Approach" to maximize the greatest outreach to all education stakeholders in Europe. In this paper we illustrate how to best reach these target groups with very specific activities to trigger and sustain enthusiasm whilst supporting the pedagogical, subject content and skill-based needs of a prescribed curriculum.

  3. One-step microlithography

    NASA Astrophysics Data System (ADS)

    Kahlen, Franz-Josef; Sankaranarayanan, Srikanth; Kar, Aravinda

    1997-09-01

    Subject of this investigation is a one-step rapid machining process to create miniaturized 3D parts, using the original sample material. An experimental setup where metal powder is fed to the laser beam-material interaction region has been built. The powder is melted and forms planar, 2D geometries as the substrate is moved under the laser beam in XY- direction. After completing the geometry in the plane, the substrate is displaced in Z-direction, and a new layer of material is placed on top of the just completed deposit. By continuous repetition of this process, 3D parts wee created. In particular, the impact of the focal spot size of the high power laser beam on the smallest achievable structures was investigated. At a translation speed of 51 mm/s a minimum material thickness of 590 micrometers was achieved. Also, it was shown that a small Z-displacement has a negligible influence on the continuity of the material deposition over this power range. A high power CO2 laser was used as energy source, the material powder under investigation was stainless steel SS304L. Helium was used as shield gas at a flow rate of 15 1/min. The incident CO2 laser beam power was varied between 300 W and 400 W, with the laser beam intensity distribute in a donut mode. The laser beam was focused to a focal diameter of 600 (Mu) m.

  4. 1992 STEP Symposium

    NASA Astrophysics Data System (ADS)

    Lui, Tony

    The year 1992 marks the quincentenary jubilee of the famous voyage of Christopher Columbus to the New World, a trip which initiated sustained contact between Europe and the American continent. Courageous explorations often lead to advancement of mankind, be they in uncharted territory or science. As much as Columbus was unaware of what lay in store for his voyage, we were just as poorly informed about what lay beyond our home planet when we began space exploration about three decades ago. There is much similarity among the pioneering spirits characteristic of both endeavors. It is thus fitting to celebrate this quincentenary occasion by declaring 1992 International Space Year (ISY).In conjunction with the COSPAR Meeting and the International Convention of the World Space Congress to be held in Washington, D.C., from August to September 1992, a 4-day symposium on the initial results from the Solar-Terrestrial Energy Program (STEP) Facilities and Theory Campaigns will be held at Johns Hopkins University, Applied Physics Laboratory, Laurel, Md., August 24-27. 1992.

  5. Step 3: Manage Your Diabetes

    MedlinePlus

    ... of this page please turn JavaScript on. Feature: Type 2 Diabetes Step 3: Manage Your Diabetes Past Issues / Fall ... you visit your health care team. Read More "Type 2 Diabetes" Articles Diabetes Is Serious But Manageable / Step 1: ...

  6. A Global Step Planning Method for Biped Robot Considering Obstacles

    NASA Astrophysics Data System (ADS)

    Tsuji, Toshiaki; Ohnishi, Kouhei

    This paper discusses about step planning of a biped robot in an environment with obstacles. Biped robot has a mechanical advantage to work in human surroundings. This is accomplished by its capability to select the discrete contact point with the ground. Though its foot placement should be discussed to step over obstacles, it is difficult because dynamic biped locomotion is a complex interaction system between upper body motion and stepping point. Applying the idea of virtual supporting point, this complex interaction is solved. The collision detection is easily achieved through modeling the obstacles to an off-limits on the horizontal plane. A stamp area is set in order to avoid the extreme stride alteration. Through these methods, the robot can prepare for the obstacle beforehand and select a series of footsteps that provides stable locomotion. Experimental results are shown to confirm the validity of the proposed methods.

  7. STEPS: JPL's Astrometric Exoplanet Survey

    NASA Technical Reports Server (NTRS)

    Shaklan, Stuart; Pravdo, Steve

    2008-01-01

    Presentation topics include: STEPS ground-based astrometry at Hale Telescope; the instrument; why astronomy and why M-dwarfs; motion of center of light about center of mass in photocentric orbit; photocentric motion vs. fractional mass; high-resolution imaging of STEPS targets; GU 802 p one possible orbit plotted with data, Keplerian frame; GJ 802 results; STEPS future; and a bibliography of STEPS papers.

  8. SPAR-H Step-by-Step Guidance

    SciTech Connect

    April M. Whaley; Dana L. Kelly; Ronald L. Boring; William J. Galyean

    2012-06-01

    Step-by-step guidance was developed recently at Idaho National Laboratory for the US Nuclear Regulatory Commission on the use of the Standardized Plant Analysis Risk-Human Reliability Analysis (SPAR-H) method for quantifying Human Failure Events (HFEs). This work was done to address SPAR-H user needs, specifically requests for additional guidance on the proper application of various aspects of the methodology. This paper overviews the steps of the SPAR-H analysis process and highlights some of the most important insights gained during the development of the step-by-step directions. This supplemental guidance for analysts is applicable when plant-specific information is available, and goes beyond the general guidance provided in existing SPAR-H documentation. The steps highlighted in this paper are: Step-1, Categorizing the HFE as Diagnosis and/or Action; Step-2, Rate the Performance Shaping Factors; Step-3, Calculate PSF-Modified HEP; Step-4, Accounting for Dependence, and; Step-5, Minimum Value Cutoff.

  9. Natural discretization of pedestrian movement in continuous space

    NASA Astrophysics Data System (ADS)

    Seitz, Michael J.; Köster, Gerta

    2012-10-01

    Is there a way to describe pedestrian movement with simple rules, as in a cellular automaton, but without being restricted to a cellular grid? Inspired by the natural stepwise movement of humans, we develop a model that uses local discretization on a circle around virtual pedestrians. This allows for movement in arbitrary directions, only limited by the chosen optimization algorithm and numerical resolution. The radii of the circles correspond to the step lengths of pedestrians and thus are model parameters, which must be derived from empirical observation. Therefore, we conducted a controlled experiment, collected empirical data for step lengths in relation with different speeds, and used the findings in our model. We complement the model with a simple calibration algorithm that allows reproducing known density-velocity relations, which constitutes a proof of concept. Further validation of the model is achieved by reenacting an evacuation scenario from experimental research. The simulated egress times match the values reported for the experiment very well. A new normalized measure for space occupancy serves to visualize the results.

  10. Natural discretization of pedestrian movement in continuous space.

    PubMed

    Seitz, Michael J; Köster, Gerta

    2012-10-01

    Is there a way to describe pedestrian movement with simple rules, as in a cellular automaton, but without being restricted to a cellular grid? Inspired by the natural stepwise movement of humans, we develop a model that uses local discretization on a circle around virtual pedestrians. This allows for movement in arbitrary directions, only limited by the chosen optimization algorithm and numerical resolution. The radii of the circles correspond to the step lengths of pedestrians and thus are model parameters, which must be derived from empirical observation. Therefore, we conducted a controlled experiment, collected empirical data for step lengths in relation with different speeds, and used the findings in our model. We complement the model with a simple calibration algorithm that allows reproducing known density-velocity relations, which constitutes a proof of concept. Further validation of the model is achieved by reenacting an evacuation scenario from experimental research. The simulated egress times match the values reported for the experiment very well. A new normalized measure for space occupancy serves to visualize the results.

  11. Convergence of discrete schemes for the Perona-Malik equation

    NASA Astrophysics Data System (ADS)

    Bellettini, G.; Novaga, M.; Paolini, M.; Tornese, C.

    We prove the convergence, up to a subsequence, of the spatial semidiscrete scheme for the one-dimensional Perona-Malik equation u=(ϕ())x, ϕ(p):=1/2 >log(1+p), when the initial datum u¯ is 1-Lipschitz out of a finite number of jump points, and we characterize the problem satisfied by the limit solution. In the more difficult case when u¯ has a whole interval where ϕ(u) is negative, we construct a solution by a careful inspection of the behaviour of the approximating solutions in a space-time neighbourhood of the jump points. The limit solution u we obtain is the same as the one obtained by replacing ϕ(ṡ) with the truncated function min(ϕ(ṡ),1), and it turns out that u solves a free boundary problem. The free boundary consists of the points dividing the region where |u|>1 from the region where |u|⩽1. Finally, we consider the full space-time discretization (implicit in time) of the Perona-Malik equation, and we show that, if the time step is small with respect to the spatial grid h, then the limit is the same as the one obtained with the spatial semidiscrete scheme. On the other hand, if the time step is large with respect to h, then the limit solution equals u¯, i.e., the standing solution of the convexified problem.

  12. Simulation of dry granular flows using discrete element methods

    NASA Astrophysics Data System (ADS)

    Martin, Hugo; Lefebvre, Aline; Maday, Yvon; Mangeney, Anne; Maury, Bertrand; Sainte-Marie, Jacques

    2017-04-01

    Granular flows are composed of interacting particles (for instance sand grains). While natural flow simulations at the field scale are generally based on continuum models, discrete element methods are very useful to get insight into the detailed contact interactions between the particles involved. We shall consider here both well known molecular dynamics (MD) and contact dynamics (CD) methods to simulate granular particle interaction. The difference between these methods is the linearisation of contact forces in MD. We are interested to compare these methods, and especially the effects of the linearisation in simulations. In the present work, we introduce a new rigid bodies model at the scale of the particles and its resolution by contact dynamics. The interesting aspect of our CD method is to treat the contacts in all the material system in one step without any iterative process required when the contacts are dealt with one after the other. All contacts are calculated here at the same time in just one iteration and the normal and tangential constraints are treated simultaneously. The present model follows from a convex optimization problem presented in [1] by B. Maury in which we add a frictional behaviour to the contact law between the particles. To analyse the behaviour of this model, we compare our results to analytical solutions when we can compute them and otherwise to simulations with molecular dynamics method. [1] A time-stepping scheme for inelastic collisions. Numerical handling of the nonoverlapping constraint, B. Maury, Numerische Mathematik, 17 january 2006.

  13. An exact and consistent adjoint method for high-fidelity discretization of the compressible flow equations

    NASA Astrophysics Data System (ADS)

    Subramanian, Ramanathan Vishnampet Ganapathi

    Methods and computing hardware advances have enabled accurate predictions of complex compressible turbulence phenomena, such as the generation of jet noise that motivates the present effort. However, limited understanding of underlying physical mechanisms restricts the utility of such predictions since they do not, by themselves, indicate a route to design improvement. Gradient-based optimization using adjoints can circumvent the flow complexity to guide designs. Such methods have enabled sensitivity analysis and active control of turbulence at engineering flow conditions by providing gradient information at computational cost comparable to that of simulating the flow. They accelerate convergence of numerical design optimization algorithms, though this is predicated on the availability of an accurate gradient of the discretized flow equations. This is challenging to obtain, since both the chaotic character of the turbulence and the typical use of discretizations near their resolution limits in order to efficiently represent its smaller scales will amplify any approximation errors made in the adjoint formulation. Formulating a practical exact adjoint that avoids such errors is especially challenging if it is to be compatible with state-of-the-art simulation methods used for the turbulent flow itself. Automatic differentiation (AD) can provide code to calculate a nominally exact adjoint, but existing general-purpose AD codes are inefficient to the point of being prohibitive for large-scale turbulence simulations. We analyze the compressible flow equations as discretized using the same high-order workhorse methods used for many high-fidelity compressible turbulence simulations, and formulate a practical space--time discrete-adjoint method without changing the basic discretization. A key step is the definition of a particular discrete analog of the continuous norm that defines our cost functional; our selection leads directly to an efficient Runge--Kutta-like scheme

  14. Friction of atomically stepped surfaces

    NASA Astrophysics Data System (ADS)

    Dikken, R. J.; Thijsse, B. J.; Nicola, L.

    2017-03-01

    The friction behavior of atomically stepped metal surfaces under contact loading is studied using molecular dynamics simulations. While real rough metal surfaces involve roughness at multiple length scales, the focus of this paper is on understanding friction of the smallest scale of roughness: atomic steps. To this end, periodic stepped Al surfaces with different step geometry are brought into contact and sheared at room temperature. Contact stress that continuously tries to build up during loading, is released with fluctuating stress drops during sliding, according to the typical stick-slip behavior. Stress release occurs not only through local slip, but also by means of step motion. The steps move along the contact, concurrently resulting in normal migration of the contact. The direction of migration depends on the sign of the step, i.e., its orientation with respect to the shearing direction. If the steps are of equal sign, there is a net migration of the entire contact accompanied by significant vacancy generation at room temperature. The stick-slip behavior of the stepped contacts is found to have all the characteristic of a self-organized critical state, with statistics dictated by step density. For the studied step geometries, frictional sliding is found to involve significant atomic rearrangement through which the contact roughness is drastically changed. This leads for certain step configurations to a marked transition from jerky sliding motion to smooth sliding, making the final friction stress approximately similar to that of a flat contact.

  15. Powerlessness Reinterpreted: Reframing Step One.

    ERIC Educational Resources Information Center

    Young, Susan L.

    The 12 steps of the well-known mutual help group, Alcoholics Anonymous (AA), begin with Step One, admitting powerlessness. Although Step One has helped many problem drinkers and other addicts, its spiritual concepts have been criticized. The possibility of reconceptualizing powerlessness as empowering, not only within AA and its offshoot programs,…

  16. Multiple stage miniature stepping motor

    DOEpatents

    Niven, William A.; Shikany, S. David; Shira, Michael L.

    1981-01-01

    A stepping motor comprising a plurality of stages which may be selectively activated to effect stepping movement of the motor, and which are mounted along a common rotor shaft to achieve considerable reduction in motor size and minimum diameter, whereby sequential activation of the stages results in successive rotor steps with direction being determined by the particular activating sequence followed.

  17. Discrete ordinates methods in xy geometry with spatially varying angular discretization

    SciTech Connect

    Bal, G.; Warin, X.

    1997-10-01

    The efficiency of a new quadrature rule adapted to the numerical resolution of a neutron transport problem in xy geometry is presented based on the use of the discrete ordinates method for the angular variable. The purpose of introducing this quadrature rule is to couple two different angular discretizations used on two nonoverlapping subdomains, which is useful for performing local refinement. This coupling and some numerical results of source problems are presented.

  18. Semiconductor step-stress testing

    NASA Technical Reports Server (NTRS)

    Meeks, H. B.; Villella, F.

    1980-01-01

    Report documents behavior of discrete diodes and transistors in extensive power and temperature overstress tests. Thirty nine devices were tested in groups designated: (1) power overstress, and (2) and (3) temperature overstress. Results are of interest to users of tested components and engineers in conduction of similar tests.

  19. Evidence for separate neural mechanisms for the timing of discrete and sustained responses.

    PubMed

    Gooch, Cynthia M; Wiener, Martin; Portugal, George S; Matell, Matthew S

    2007-07-02

    Methamphetamine (MAP), an indirect dopamine agonist, has been shown to produce a leftward shift in the time of responding under operant response protocols that encourage repetitive responding (e.g., lever pressing). Given the involvement of striatal dopamine activity in the control of discrete motor behavior, as well as in the timing of these responses, an important question arises as to whether a dissociation is possible between changes in the timing of discrete responding and timing of other behaviors. Rats were trained on a modified peak-interval (PI) procedure such that reward was contingent upon the presence of the animal's snout in a nosepoke apparatus at the target time, as an alternative to the typical requirement of a discrete head entry response. Thus spatial selection, but not necessarily motor behavior, at the appropriate time was required to receive a reward. Rats were given MAP in one of 3 doses (0.5, 1.0, or 1.5 mg/kg), or a saline control injection before PI sessions to determine whether the drug elicits a dose-dependent effect on timing of spatial position, as it has been shown to do for discrete behaviors. Following administration of MAP, the peak time of the proportion of time spent in the nosepoke did not change, while the peak time of the rate of response shifted to the left. Single-trial analysis revealed a similar pattern: Position of response step functions defined by being in the nosepoke did not shift, but step functions based on response rate changed with increasing doses of MAP. These data support a model of multiple timing processes controlling different behaviors, at least one of which is specific to discrete motor behavior and is modifiable by dopamine.

  20. Step-by-step guide to critiquing research. Part 2: Qualitative research.

    PubMed

    Ryan, Frances; Coughlan, Michael; Cronin, Patricia

    As with a quantitative study, critical analysis of a qualitative study involves an in-depth review of how each step of the research was undertaken. Qualitative and quantitative studies are, however, fundamentally different approaches to research and therefore need to be considered differently with regard to critiquing. The different philosophical underpinnings of the various qualitative research methods generate discrete ways of reasoning and distinct terminology; however, there are also many similarities within these methods. Because of this and its subjective nature, qualitative research it is often regarded as more difficult to critique. Nevertheless, an evidenced-based profession such as nursing cannot accept research at face value, and nurses need to be able to determine the strengths and limitations of qualitative as well as quantitative research studies when reviewing the available literature on a topic.

  1. Step-step interactions on GaAs (110) nanopatterns

    SciTech Connect

    Galiana, B.; Benedicto, M.; Tejedor, P.

    2013-01-14

    The step-step interactions on vicinal GaAs (110) surface patterns have been extracted from the quantitative analysis of the terrace width distribution (TWD). We have specifically studied the interactions in near-equilibrium faceting and kinetics-driven step bunching and meandering formed by spontaneous self-organization or through the modification of GaAs growth kinetics by atomic hydrogen. We show that the experimental TWDs determined from atomic force microscopy measurements can be accurately described by a weighed sum of a generalized Wigner distribution and several Gaussians. The results of our calculations indicate that straight facets are formed during high temperature homoepitaxy due to attractive interactions between [110] steps. At low temperatures, steady state attractive interactions in [110] step bunches are preceded by a transition regime dominated by entropic and energetic repulsions between meandering [11n]-type steps (n {>=} 2), whose population density exceeds that of the [110] bunched steps. In addition, it has been found that atomic H reduces the attractive interactions between [110] bunched steps and enhances entropic and dipole-induced energetic repulsions between H-terminated [11n] steps through the inhibition of As-As bond formation at step edges. Our analysis has evidenced a correlation between the value of the adjustable parameter that accounts in our model for the specific weight of the secondary peaks in the TWD ({beta}) and the extent of transverse meandering on the vicinal surface.

  2. Discrete-time linear and nonlinear aerodynamic impulse responses for efficient CFD analyses

    NASA Astrophysics Data System (ADS)

    Silva, Walter Arturo

    This dissertation discusses the mathematical existence and the numerical identification of linear and nonlinear aerodynamic impulse response functions. Differences between continuous-time and discrete-time system theories, which permit the identification and efficient use of these functions, will be detailed. Important input/output definitions and the concept of linear and nonlinear systems with memory will also be discussed. It will be shown that indicial (step or steady) responses (such as Wagner's function), forced harmonic responses (such as Theodorsen's function or those from doublet lattice theory), and responses to random inputs (such as gusts) can all be obtained from an aerodynamic impulse response function. This will establish the aerodynamic discrete-time impulse response function as the most fundamental and computationally efficient aerodynamic function that can be extracted from any given discrete-time, aerodynamic system. The results presented in this dissertation help to unify the understanding of classical two-dimensional continuous-time theories with modern three-dimensional, discrete-time theories. Nonlinear aerodynamic impulse responses are identified using the Volterra theory of nonlinear systems. The theory is described and a discrete-time kernel identification technique is presented. The kernel identification technique is applied to a simple nonlinear circuit for illustrative purposes. The method is then applied to the nonlinear viscous Burger's equation as an example of an application to a simple CFD model. Finally, the method is applied to a three-dimensional aeroelastic model using the CAP-TSD (Computational Aeroelasticity Program - Transonic Small Disturbance) code and then to a two-dimensional model using the CFL3D Navier-Stokes code. Comparisons of accuracy and computational cost savings are presented. Because of its mathematical generality, an important attribute of this methodology is that it is applicable to a wide range of nonlinear

  3. An Arbitrary Lagrangian-Eulerian Discretization of MHD on 3D Unstructured Grids

    SciTech Connect

    Rieben, R N; White, D A; Wallin, B K; Solberg, J M

    2006-06-12

    We present an arbitrary Lagrangian-Eulerian (ALE) discretization of the equations of resistive magnetohydrodynamics (MHD) on unstructured hexahedral grids. The method is formulated using an operator-split approach with three distinct phases: electromagnetic diffusion, Lagrangian motion, and Eulerian advection. The resistive magnetic dynamo equation is discretized using a compatible mixed finite element method with a 2nd order accurate implicit time differencing scheme which preserves the divergence-free nature of the magnetic field. At each discrete time step, electromagnetic force and heat terms are calculated and coupled to the hydrodynamic equations to compute the Lagrangian motion of the conducting materials. By virtue of the compatible discretization method used, the invariants of Lagrangian MHD motion are preserved in a discrete sense. When the Lagrangian motion of the mesh causes significant distortion, that distortion is corrected with a relaxation of the mesh, followed by a 2nd order monotonic remap of the electromagnetic state variables. The remap is equivalent to Eulerian advection of the magnetic flux density with a fictitious mesh relaxation velocity. The magnetic advection is performed using a novel variant of constrained transport (CT) that is valid for unstructured hexahedral grids with arbitrary mesh velocities. The advection method maintains the divergence free nature of the magnetic field and is second order accurate in regions where the solution is sufficiently smooth. For regions in which the magnetic field is discontinuous (e.g. MHD shocks) the method is limited using a novel variant of algebraic flux correction (AFC) which is local extremum diminishing (LED) and divergence preserving. Finally, we verify each stage of the discretization via a set of numerical experiments.

  4. Ecological monitoring in a discrete-time prey-predator model.

    PubMed

    Gámez, M; López, I; Rodríguez, C; Varga, Z; Garay, J

    2017-09-21

    The paper is aimed at the methodological development of ecological monitoring in discrete-time dynamic models. In earlier papers, in the framework of continuous-time models, we have shown how a systems-theoretical methodology can be applied to the monitoring of the state process of a system of interacting populations, also estimating certain abiotic environmental changes such as pollution, climatic or seasonal changes. In practice, however, there may be good reasons to use discrete-time models. (For instance, there may be discrete cycles in the development of the populations, or observations can be made only at discrete time steps.) Therefore the present paper is devoted to the development of the monitoring methodology in the framework of discrete-time models of population ecology. By monitoring we mean that, observing only certain component(s) of the system, we reconstruct the whole state process. This may be necessary, e.g., when in a complex ecosystem the observation of the densities of certain species is impossible, or too expensive. For the first presentation of the offered methodology, we have chosen a discrete-time version of the classical Lotka-Volterra prey-predator model. This is a minimal but not trivial system where the methodology can still be presented. We also show how this methodology can be applied to estimate the effect of an abiotic environmental change, using a component of the population system as an environmental indicator. Although this approach is illustrated in a simplest possible case, it can be easily extended to larger ecosystems with several interacting populations and different types of abiotic environmental effects. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. A variable step incremental procedure. [for nonlinear equations in finite element structural analysis

    NASA Technical Reports Server (NTRS)

    Thomas, G. R.

    1973-01-01

    Description of a variable step incremental procedure for the solution of nonlinear equations in finite element structural analysis. The proposed procedure is effective in improving the accuracy of the basic incremental technique and in providing, in addition, an accurate estimate of the discretization error. The proposed approach is highly appropriate for solving problems for which the user has no a priori estimate of the step size to use.

  6. Refined BCF-type boundary conditions for mesoscale surface step dynamics

    SciTech Connect

    Zhao, Renjie; Ackerman, David M.; Evans, James W.

    2015-06-24

    Deposition on a vicinal surface with alternating rough and smooth steps is described by a solid-on-solid model with anisotropic interactions. Kinetic Monte Carlo (KMC) simulations of the model reveal step pairing in the absence of any additional step attachment barriers. We explore the description of this behavior within an analytic Burton-Cabrera-Frank (BCF)-type step dynamics treatment. Without attachment barriers, conventional kinetic coefficients for the rough and smooth steps are identical, as are the predicted step velocities for a vicinal surface with equal terrace widths. However, we determine refined kinetic coefficients from a two-dimensional discrete deposition-diffusion equation formalism which accounts for step structure. These coefficients are generally higher for rough steps than for smooth steps, reflecting a higher propensity for capture of diffusing terrace adatoms due to a higher kink density. Such refined coefficients also depend on the local environment of the step and can even become negative (corresponding to net detachment despite an excess adatom density) for a smooth step in close proximity to a rough step. Incorporation of these refined kinetic coefficients into a BCF-type step dynamics treatment recovers quantitatively the mesoscale step-pairing behavior observed in the KMC simulations.

  7. Refined BCF-type boundary conditions for mesoscale surface step dynamics

    NASA Astrophysics Data System (ADS)

    Zhao, Renjie; Ackerman, David M.; Evans, James W.

    2015-06-01

    Deposition on a vicinal surface with alternating rough and smooth steps is described by a solid-on-solid model with anisotropic interactions. Kinetic Monte Carlo (KMC) simulations of the model reveal step pairing in the absence of any additional step attachment barriers. We explore the description of this behavior within an analytic Burton-Cabrera-Frank (BCF)-type step dynamics treatment. Without attachment barriers, conventional kinetic coefficients for the rough and smooth steps are identical, as are the predicted step velocities for a vicinal surface with equal terrace widths. However, we determine refined kinetic coefficients from a two-dimensional discrete deposition-diffusion equation formalism which accounts for step structure. These coefficients are generally higher for rough steps than for smooth steps, reflecting a higher propensity for capture of diffusing terrace adatoms due to a higher kink density. Such refined coefficients also depend on the local environment of the step and can even become negative (corresponding to net detachment despite an excess adatom density) for a smooth step in close proximity to a rough step. Our key observation is that incorporation of these refined kinetic coefficients into a BCF-type step dynamics treatment recovers quantitatively the mesoscale step-pairing behavior observed in the KMC simulations.

  8. Semi-Discrete Ingham-Type Inequalities

    SciTech Connect

    Komornik, Vilmos Loreti, Paola

    2007-03-15

    One of the general methods in linear control theory is based on harmonic and non-harmonic Fourier series. The key of this approach is the establishment of various suitable adaptations and generalizations of the classical Parseval equality. A new and systematic approach was begun in our papers in collaboration with Baiocchi. Many recent results of this kind, obtained through various Ingham-type theorems, were exposed recently. Although this work concentrated on continuous models, in connection with numerical simulations a natural question is whether these results also admit useful discrete versions. The purpose of this paper is to establish discrete versions of various Ingham-type theorems by using our approach. They imply the earlier continuous results by a simple limit process.

  9. Discrete shaped strain sensors for intelligent structures

    NASA Technical Reports Server (NTRS)

    Andersson, Mark S.; Crawley, Edward F.

    1992-01-01

    Design of discrete, highly distributed sensor systems for intelligent structures has been studied. Data obtained indicate that discrete strain-averaging sensors satisfy the functional requirements for distributed sensing of intelligent structures. Bartlett and Gauss-Hanning sensors, in particular, provide good wavenumber characteristics while meeting the functional requirements. They are characterized by good rolloff rates and positive Fourier transforms for all wavenumbers. For the numerical integration schemes, Simpson's rule is considered to be very simple to implement and consistently provides accurate results for five sensors or more. It is shown that a sensor system that satisfies the functional requirements can be applied to a structure that supports mode shapes with purely sinusoidal curvature.

  10. Impending failure detection for a discrete process

    NASA Astrophysics Data System (ADS)

    Chen, Yubao

    1993-03-01

    Signals from a discrete process contain a strong modulation as a result of the discrete events in the process, such as paper passage in a recirculating document feeder (RDF). This paper presents a study of the methodology of process monitoring for a RDF system. A fault tree has been established that shows the cause-and-effect relationship regarding possible malfunctions of a RDF system. Critical components of the RDF system have been identified for condition monitoring. The signature from the measurements of position, vibration, vacuum pressure, and drive motor current have been analysed. A data separation scheme was used in signal processing to demodulate the strong signal component associated with paper passage. Unique index extraction algorithms based on time series analysis and modeling have been developed to detect failures of these components. A decision-making scheme based on multiple voting has been implemented.

  11. Using discrete Tchebichef transform on speech recognition

    NASA Astrophysics Data System (ADS)

    Ernawan, Ferda; Noersasongko, Edi; Abu, Nur Azman

    2011-12-01

    Speech recognition is becoming popular in current development on mobile devices. Presumably, mobile devices have limited computational power, memory size and battery life. In general, speech recognition is a heavy process that required large sample data within each window. Fast Fourier Transform (FFT) is the most popular transform in speech recognition. In addition, FFT operates in complex field with imaginary numbers. This paper proposes an approach based on discrete orthonormal Tchebichef polynomials as a possible alternative to FFT. Discrete Tchebichef Transform (DTT) shall be utilized here instead of FFT. The preliminary experimental result shows that speech recognition using DTT produces a simpler and efficient transformation for speech recognition. The frequency formants using FFT and DTT have been compared. The result showed that, they have produced relatively identical output in term of basic vowel and consonant recognition. DTT has the potential to provide simpler computing with DTT coefficient real numbers only than FFT on speech recognition.

  12. Optimal Discretization Resolution in Algebraic Image Reconstruction

    NASA Astrophysics Data System (ADS)

    Sharif, Behzad; Kamalabadi, Farzad

    2005-11-01

    In this paper, we focus on data-limited tomographic imaging problems where the underlying linear inverse problem is ill-posed. A typical regularized reconstruction algorithm uses algebraic formulation with a predetermined discretization resolution. If the selected resolution is too low, we may loose useful details of the underlying image and if it is too high, the reconstruction will be unstable and the representation will fit irrelevant features. In this work, two approaches are introduced to address this issue. The first approach is using Mallow's CL method or generalized cross-validation. For each of the two methods, a joint estimator of regularization parameter and discretization resolution is proposed and their asymptotic optimality is investigated. The second approach is a Bayesian estimator of the model order using a complexity-penalizing prior. Numerical experiments focus on a space imaging application from a set of limited-angle tomographic observations.

  13. Discrete Abelian gauge symmetries and axions

    NASA Astrophysics Data System (ADS)

    Honecker, Gabriele; Staessens, Wieland

    2015-07-01

    We combine two popular extensions of beyond the Standard Model physics within the framework of intersecting D6-brane models: discrete ℤn symmetries and Peccei-Quinn axions. The underlying natural connection between both extensions is formed by the presence of massive U(1) gauge symmetries in D-brane model building. Global intersecting D6-brane models on toroidal orbifolds of the type T6/ℤ2N and T6/ℤ2 × ℤ2M with discrete torsion offer excellent playgrounds for realizing these extensions. A generation-dependent ℤ2 symmetry is identified in a global Pati-Salam model, while global left-right symmetric models give rise to supersymmetric realizations of the DFSZ axion model. In one class of the latter models, the axion as well as Standard Model particles carry a non-trivial ℤ3 charge.

  14. Discrete Spectrum Reconstruction Using Integral Approximation Algorithm.

    PubMed

    Sizikov, Valery; Sidorov, Denis

    2017-07-01

    An inverse problem in spectroscopy is considered. The objective is to restore the discrete spectrum from observed spectrum data, taking into account the spectrometer's line spread function. The problem is reduced to solution of a system of linear-nonlinear equations (SLNE) with respect to intensities and frequencies of the discrete spectral lines. The SLNE is linear with respect to lines' intensities and nonlinear with respect to the lines' frequencies. The integral approximation algorithm is proposed for the solution of this SLNE. The algorithm combines solution of linear integral equations with solution of a system of linear algebraic equations and avoids nonlinear equations. Numerical examples of the application of the technique, both to synthetic and experimental spectra, demonstrate the efficacy of the proposed approach in enabling an effective enhancement of the spectrometer's resolution.

  15. Discrete coherent states for higher Landau levels

    NASA Astrophysics Data System (ADS)

    Abreu, L. D.; Balazs, P.; de Gosson, M.; Mouayn, Z.

    2015-12-01

    We consider the quantum dynamics of a charged particle evolving under the action of a constant homogeneous magnetic field, with emphasis on the discrete subgroups of the Heisenberg group (in the Euclidean case) and of the SL(2 , R) group (in the Hyperbolic case). We investigate completeness properties of discrete coherent states associated with higher order Euclidean and hyperbolic Landau levels, partially extending classic results of Perelomov and of Bargmann, Butera, Girardello and Klauder. In the Euclidean case, our results follow from identifying the completeness problem with known results from the theory of Gabor frames. The results for the hyperbolic setting follow by using a combination of methods from coherent states, time-scale analysis and the theory of Fuchsian groups and their associated automorphic forms.

  16. Fluid Coupling in a Discrete Cochlear Model

    NASA Astrophysics Data System (ADS)

    Elliott, S. J.; Lineton, B.; Ni, G.

    2011-11-01

    The interaction between the basilar membrane, BM, dynamics and the fluid coupling in the cochlea can be formulated using a discrete model by assuming that the BM is divided into a number of longitudinal elements. The form of the fluid coupling can then be understood by dividing it into a far field component, due to plane wave acoustic coupling, and a near field component, due to higher order evanescent acoustic modes. The effects of non-uniformity and asymmetry in the cross-sectional areas of the fluid chambers can also be accounted for within this formulation. The discrete model is used to calculate the effect on the coupled BM response of a short cochlear implant, which reduces the volume of one of the fluid chambers over about half its length. The passive response of the coupled cochlea at lower frequencies is shown to be almost unaffected by this change in volume.

  17. Hydraulically controlled discrete sampling from open boreholes

    USGS Publications Warehouse

    Harte, Philip T.

    2013-01-01

    Groundwater sampling from open boreholes in fractured-rock aquifers is particularly challenging because of mixing and dilution of fluid within the borehole from multiple fractures. This note presents an alternative to traditional sampling in open boreholes with packer assemblies. The alternative system called ZONFLO (zonal flow) is based on hydraulic control of borehole flow conditions. Fluid from discrete fractures zones are hydraulically isolated allowing for the collection of representative samples. In rough-faced open boreholes and formations with less competent rock, hydraulic containment may offer an attractive alternative to physical containment with packers. Preliminary test results indicate a discrete zone can be effectively hydraulically isolated from other zones within a borehole for the purpose of groundwater sampling using this new method.

  18. Multiple Autonomous Discrete Event Controllers for Constellations

    NASA Technical Reports Server (NTRS)

    Esposito, Timothy C.

    2003-01-01

    The Multiple Autonomous Discrete Event Controllers for Constellations (MADECC) project is an effort within the National Aeronautics and Space Administration Goddard Space Flight Center's (NASA/GSFC) Information Systems Division to develop autonomous positioning and attitude control for constellation satellites. It will be accomplished using traditional control theory and advanced coordination algorithms developed by the Johns Hopkins University Applied Physics Laboratory (JHU/APL). This capability will be demonstrated in the discrete event control test-bed located at JHU/APL. This project will be modeled for the Leonardo constellation mission, but is intended to be adaptable to any constellation mission. To develop a common software architecture. the controllers will only model very high-level responses. For instance, after determining that a maneuver must be made. the MADECC system will output B (Delta)V (velocity change) value. Lower level systems must then decide which thrusters to fire and for how long to achieve that (Delta)V.

  19. Discrete breathers in a polyethylene chain

    NASA Astrophysics Data System (ADS)

    Savin, A. V.; Manevitch, L. I.

    2003-04-01

    The existence of discrete breathers or intrinsic localized modes (localized periodic oscillations of transzigzag) is shown. In the localization region periodic contraction-extension of valence C—C bonds occurs, which is accompanied by decrease-increase of valence angles. The concentration of thermally activated formation of discrete breathers in the chain has to increase when temperature grows. However, the breather in thermalized chain has a finite time of life decreasing with growth of the temperature. It is a reason for nonmonotonous dependence of concentration breathers upon temperature T. Concentration increases for small temperature (T<200 K) and decreases for large temperature (T>200 K) with maximal magnitude for T=200 K. One can identify the revealed breathers as a unique type of stable localized periodic elementary excitation in thermalized polymer crystal existing even for sufficiently small temperature and contributing essentially to heat capacity.

  20. Special relativity in a discrete quantum universe

    NASA Astrophysics Data System (ADS)

    Bisio, Alessandro; D'Ariano, Giacomo Mauro; Perinotti, Paolo

    2016-10-01

    The hypothesis of a discrete fabric of the universe, the "Planck scale," is always on stage since it solves mathematical and conceptual problems in the infinitely small. However, it clashes with special relativity, which is designed for the continuum. Here, we show how the clash can be overcome within a discrete quantum theory where the evolution of fields is described by a quantum cellular automaton. The reconciliation is achieved by defining the change of observer as a change of representation of the dynamics, without any reference to space-time. We use the relativity principle, i.e., the invariance of dynamics under change of inertial observer, to identify a change of inertial frame with a symmetry of the dynamics. We consider the full group of such symmetries, and recover the usual Lorentz group in the relativistic regime of low energies, while at the Planck scale the covariance is nonlinearly distorted.

  1. Discrete shaped strain sensors for intelligent structures

    NASA Technical Reports Server (NTRS)

    Andersson, Mark S.; Crawley, Edward F.

    1992-01-01

    Design of discrete, highly distributed sensor systems for intelligent structures has been studied. Data obtained indicate that discrete strain-averaging sensors satisfy the functional requirements for distributed sensing of intelligent structures. Bartlett and Gauss-Hanning sensors, in particular, provide good wavenumber characteristics while meeting the functional requirements. They are characterized by good rolloff rates and positive Fourier transforms for all wavenumbers. For the numerical integration schemes, Simpson's rule is considered to be very simple to implement and consistently provides accurate results for five sensors or more. It is shown that a sensor system that satisfies the functional requirements can be applied to a structure that supports mode shapes with purely sinusoidal curvature.

  2. Quantum RLC circuits: Charge discreteness and resonance

    NASA Astrophysics Data System (ADS)

    Utreras-Díaz, Constantino A.

    2008-10-01

    In a recent article [C.A. Utreras-Díaz, Phys. Lett. A 372 (2008) 5059], we have advanced a semiclassical theory of quantum circuits with discrete charge and electrical resistance. In this work, we present a few elementary applications of this theory. For the zero resistance inductive circuit, we obtain the Stark ladder energies in yet another way; for the circuit driven by a combination d.c. plus a.c. electromotive force (emf) we generalize earlier results by Chandía et al. [K. Chandía, J.C. Flores, E. Lazo, Phys. Lett. A 359 (2006) 693]. As a second application, we investigate the effect of electrical resistance and charge discreteness, in the resonance conditions of a series RLC quantum circuit.

  3. Short-term time step convergence in a climate model.

    PubMed

    Wan, Hui; Rasch, Philip J; Taylor, Mark A; Jablonowski, Christiane

    2015-03-01

    This paper evaluates the numerical convergence of very short (1 h) simulations carried out with a spectral-element (SE) configuration of the Community Atmosphere Model version 5 (CAM5). While the horizontal grid spacing is fixed at approximately 110 km, the process-coupling time step is varied between 1800 and 1 s to reveal the convergence rate with respect to the temporal resolution. Special attention is paid to the behavior of the parameterized subgrid-scale physics. First, a dynamical core test with reduced dynamics time steps is presented. The results demonstrate that the experimental setup is able to correctly assess the convergence rate of the discrete solutions to the adiabatic equations of atmospheric motion. Second, results from full-physics CAM5 simulations with reduced physics and dynamics time steps are discussed. It is shown that the convergence rate is 0.4-considerably slower than the expected rate of 1.0. Sensitivity experiments indicate that, among the various subgrid-scale physical parameterizations, the stratiform cloud schemes are associated with the largest time-stepping errors, and are the primary cause of slow time step convergence. While the details of our findings are model specific, the general test procedure is applicable to any atmospheric general circulation model. The need for more accurate numerical treatments of physical parameterizations, especially the representation of stratiform clouds, is likely common in many models. The suggested test technique can help quantify the time-stepping errors and identify the related model sensitivities.

  4. Scalable wavelet-based active network detection of stepping stones

    NASA Astrophysics Data System (ADS)

    Gilbert, Joseph I.; Robinson, David J.; Butts, Jonathan W.; Lacey, Timothy H.

    2012-06-01

    Network intrusions leverage vulnerable hosts as stepping stones to penetrate deeper into a network and mask malicious actions from detection. Identifying stepping stones presents a significant challenge because network sessions appear as legitimate traffic. This research focuses on a novel active watermark technique using discrete wavelet transformations to mark and detect interactive network sessions. This technique is scalable, resilient to network noise, and difficult for attackers to discern that it is in use. Previously captured timestamps from the CAIDA 2009 dataset are sent using live stepping stones in the Amazon Elastic Compute Cloud service. The client system sends watermarked and unmarked packets from California to Virginia using stepping stones in Tokyo, Ireland and Oregon. Five trials are conducted in which the system sends simultaneous watermarked samples and unmarked samples to each target. The live experiment results demonstrate approximately 5% False Positive and 5% False Negative detection rates. Additionally, watermark extraction rates of approximately 92% are identified for a single stepping stone. The live experiment results demonstrate the effectiveness of discerning watermark traffic as applied to identifying stepping stones.

  5. DOS: the discrete-ordinates system. [LMFBR

    SciTech Connect

    Rhoades, W. A.; Emmett, M. B.

    1982-09-01

    The Discrete Ordinates System determines the flux of neutrons or photons due either to fixed sources specified by the user or to sources generated by particle interaction with the problem materials. It also determines numerous secondary results which depend upon flux. Criticality searches can be performed. Numerous input, output, and file manipulation facilities are provided. The DOS driver program reads the problem specification from an input file and calls various program modules into execution as specified by the input file.

  6. Computational requirements for a discrete Kalman filter.

    NASA Technical Reports Server (NTRS)

    Mendel, J. M.

    1971-01-01

    Computational requirements - i.e., computing time per cycle (iteration) and required storage - which determine minimum sampling rates and computer memory size, were obtained as functions of the dimensions of the important system matrices for a discrete Kalman filter. Two types of measurement processing are discussed: simultaneous and sequential. It is shown that it is often better to process statistically independent measurements in more than one batch and then use sequential processing than to process them together via simultaneous processing.

  7. Discrete sequence prediction and its applications

    NASA Technical Reports Server (NTRS)

    Laird, Philip

    1992-01-01

    Learning from experience to predict sequences of discrete symbols is a fundamental problem in machine learning with many applications. We apply sequence prediction using a simple and practical sequence-prediction algorithm, called TDAG. The TDAG algorithm is first tested by comparing its performance with some common data compression algorithms. Then it is adapted to the detailed requirements of dynamic program optimization, with excellent results.

  8. Performance of the discrete electrode railgun

    SciTech Connect

    Usuba, S.; Kakudate, Y.; Yoshida, M.; Aoki, K.; Yamawaki, H.; Fujiwara, S. ); Miyamoto, M. ); Kubota, A. ); Den, M. )

    1991-01-01

    In this paper a concept of Discrete Electrode (DE) railgun is presented. Plasma acceleration experiments using small DE railguns showed that the DE railgun could regulate the armature current distribution by means of fuses and a velocity of the armature current propagation was significantly higher than that in a normal type railgun. A mechanism of the armature current propagation in the DE railgun was discussed.

  9. Discrete Bimodal Probes for Thrombus Imaging

    PubMed Central

    Uppal, Ritika; Ciesienski, Kate L.; Chonde, Daniel B.; Loving, Galen S.; Caravan, Peter

    2012-01-01

    Here we report a generalizable solid/solution phase strategy for the synthesis of discrete bimodal fibrin-targeted imaging probes. A fibrin-specific peptide was conjugated with two distinct imaging reporters at the C- and N-terminus. In vitro studies demonstrated retention of fibrin affinity and specificity. Imaging studies showed that these probes could detect fibrin over a wide range of probe concentrations by optical, magnetic resonance, and positron emission tomography imaging. PMID:22698259

  10. Discrete Atomic Layers at the Molecular Level

    NASA Astrophysics Data System (ADS)

    Yorimitsu, Hideki; Bhanuchandra, M.

    2015-12-01

    In this review, we deal with the syntheses of large discrete atomic layers at the molecular level. Spectroscopic measurements as well as X-ray crystallographic analyses lead to unambiguous characterizations of these layers. The molecular atomic layers can be considered to be parts of graphenes and related atomic layers, thereby helping to understand such indefinitely huge atomic layers or serving as seeds for the controlled synthesis of nanocarbons.

  11. Program For Parallel Discrete-Event Simulation

    NASA Technical Reports Server (NTRS)

    Beckman, Brian C.; Blume, Leo R.; Geiselman, John S.; Presley, Matthew T.; Wedel, John J., Jr.; Bellenot, Steven F.; Diloreto, Michael; Hontalas, Philip J.; Reiher, Peter L.; Weiland, Frederick P.

    1991-01-01

    User does not have to add any special logic to aid in synchronization. Time Warp Operating System (TWOS) computer program is special-purpose operating system designed to support parallel discrete-event simulation. Complete implementation of Time Warp mechanism. Supports only simulations and other computations designed for virtual time. Time Warp Simulator (TWSIM) subdirectory contains sequential simulation engine interface-compatible with TWOS. TWOS and TWSIM written in, and support simulations in, C programming language.

  12. An essay on discrete foundations for physics

    SciTech Connect

    Noyes, H.P.; McGoveran, D.O.

    1988-07-01

    We base our theory of physics and cosmology on the five principles of finiteness, discreteness, finite computability, absolute non-uniqueness, and strict construction. Our modeling methodology starts from the current practice of physics, constructs a self-consistent representation based on the ordering operator calculus and provides rules of correspondence that allow us to test the theory by experiment. We use program universe to construct a growing collection of bit strings whose initial portions (labels) provide the quantum numbers that are conserved in the events defined by the construction. The labels are followed by content strings which are used to construct event-based finite and discrete coordinates. On general grounds such a theory has a limiting velocity, and positions and velocities do not commute. We therefore reconcile quantum mechanics with relativity at an appropriately fundamental stage in the construction. We show that events in different coordinate systems are connected by the appropriate finite and discrete version of the Lorentz transformation, that 3-momentum is conserved in events, and that this conservation law is the same as the requirement that different paths can ''interfere'' only when they differ by an integral number of deBroglie wavelengths. 38 refs., 12 figs., 3 tabs.

  13. Generalized Detectability for Discrete Event Systems

    PubMed Central

    Shu, Shaolong; Lin, Feng

    2011-01-01

    In our previous work, we investigated detectability of discrete event systems, which is defined as the ability to determine the current and subsequent states of a system based on observation. For different applications, we defined four types of detectabilities: (weak) detectability, strong detectability, (weak) periodic detectability, and strong periodic detectability. In this paper, we extend our results in three aspects. (1) We extend detectability from deterministic systems to nondeterministic systems. Such a generalization is necessary because there are many systems that need to be modeled as nondeterministic discrete event systems. (2) We develop polynomial algorithms to check strong detectability. The previous algorithms are based on observer whose construction is of exponential complexity, while the new algorithms are based on a new automaton called detector. (3) We extend detectability to D-detectability. While detectability requires determining the exact state of a system, D-detectability relaxes this requirement by asking only to distinguish certain pairs of states. With these extensions, the theory on detectability of discrete event systems becomes more applicable in solving many practical problems. PMID:21691432

  14. Discrete Deterministic and Stochastic Petri Nets

    NASA Technical Reports Server (NTRS)

    Zijal, Robert; Ciardo, Gianfranco

    1996-01-01

    Petri nets augmented with timing specifications gained a wide acceptance in the area of performance and reliability evaluation of complex systems exhibiting concurrency, synchronization, and conflicts. The state space of time-extended Petri nets is mapped onto its basic underlying stochastic process, which can be shown to be Markovian under the assumption of exponentially distributed firing times. The integration of exponentially and non-exponentially distributed timing is still one of the major problems for the analysis and was first attacked for continuous time Petri nets at the cost of structural or analytical restrictions. We propose a discrete deterministic and stochastic Petri net (DDSPN) formalism with no imposed structural or analytical restrictions where transitions can fire either in zero time or according to arbitrary firing times that can be represented as the time to absorption in a finite absorbing discrete time Markov chain (DTMC). Exponentially distributed firing times are then approximated arbitrarily well by geometric distributions. Deterministic firing times are a special case of the geometric distribution. The underlying stochastic process of a DDSPN is then also a DTMC, from which the transient and stationary solution can be obtained by standard techniques. A comprehensive algorithm and some state space reduction techniques for the analysis of DDSPNs are presented comprising the automatic detection of conflicts and confusions, which removes a major obstacle for the analysis of discrete time models.

  15. Perturbation theory for multipolar discrete fluids.

    PubMed

    Benavides, Ana L; Gámez, Francisco

    2011-10-07

    An analytical expression for the Helmholtz free energy of discrete multipolar potentials as a function of density, temperature, and intermolecular parameters is obtained as an extension of the multipolar square-well perturbation theory [A. L. Benavides, Y. Guevara, and F. del Río, Physica A 202, 420 (1994)]. The presented procedure is suitable for the description of a more general intermolecular potential model taking into account the overlap and dispersion forces through a discrete potential represented by a sequence of square-shoulders and wells, as well as electrostatic interactions. The main advantage of this approach is that since the Helmholtz free energy is given as an explicit expression in terms of the intermolecular parameters characterizing the interaction, the properties of interest can be easily obtained through usual thermodynamic relations. Besides, since a great variety of discretized potentials can be used with this equation of state, its applicability is very vast. By varying the intermolecular parameters, some illustrative cases are considered, and their phase diagrams are tested against available simulation data. It is found that this theoretical approach is able to reproduce qualitatively and quantitatively well the vapor-liquid equilibrium of the chosen potentials with different multipole moment of varied strengths, except in the critical region. © 2011 American Institute of Physics

  16. Perturbation theory for multipolar discrete fluids

    NASA Astrophysics Data System (ADS)

    Benavides, Ana L.; Gámez, Francisco

    2011-10-01

    An analytical expression for the Helmholtz free energy of discrete multipolar potentials as a function of density, temperature, and intermolecular parameters is obtained as an extension of the multipolar square-well perturbation theory [A. L. Benavides, Y. Guevara, and F. del Río, Physica A 202, 420 (1994), 10.1016/0378-4371(94)90469-3]. The presented procedure is suitable for the description of a more general intermolecular potential model taking into account the overlap and dispersion forces through a discrete potential represented by a sequence of square-shoulders and wells, as well as electrostatic interactions. The main advantage of this approach is that since the Helmholtz free energy is given as an explicit expression in terms of the intermolecular parameters characterizing the interaction, the properties of interest can be easily obtained through usual thermodynamic relations. Besides, since a great variety of discretized potentials can be used with this equation of state, its applicability is very vast. By varying the intermolecular parameters, some illustrative cases are considered, and their phase diagrams are tested against available simulation data. It is found that this theoretical approach is able to reproduce qualitatively and quantitatively well the vapor-liquid equilibrium of the chosen potentials with different multipole moment of varied strengths, except in the critical region.

  17. Dynamical Properties of Discrete Reaction Networks

    PubMed Central

    Paulevé, Loïc; Craciun, Gheorghe; Koeppl, Heinz

    2013-01-01

    Reaction networks are commonly used to model the dynamics of populations subject to transformations that follow an imposed stoichiometry. This paper focuses on the efficient characterisation of dynamical properties of Discrete Reaction Networks (DRNs). DRNs can be seen as modeling the underlying discrete nondeterministic transitions of stochastic models of reaction networks. In that sense, a proof of non-reachability in a given DRN has immediate implications for any concrete stochastic model based on that DRN, independent of the choice of kinetic laws and constants. Moreover, if we assume that stochastic kinetic rates are given by the mass-action law (or any other kinetic law that gives non-vanishing probability to each reaction if the required number of interacting substrates is present), then reachability properties are equivalent in the two settings. The analysis of two types of global dynamical properties of DRNs is addressed: irreducibility, i.e., the ability to reach any discrete state from any other state; and recurrence, i.e., the ability to return to any initial state. Our results consider both the verification of such properties when species are present in a large copy number, and in the general case. The necessary and sufficient conditions obtained involve algebraic conditions on the network reactions which in most cases can be verified using linear programming. Finally, the relationship of DRN irreducibility and recurrence with dynamical properties of stochastic and continuous models of reaction networks is discussed. PMID:23722628

  18. Police investigations: discretion denied yet undeniably exercised

    PubMed Central

    Belur, J.; Tilley, N.; Osrin, D.; Daruwalla, N.; Kumar, M.; Tiwari, V.

    2014-01-01

    Police investigations involve determining whether a crime has been committed, and if so what type of crime, who has committed it and whether there is the evidence to charge the perpetrators. Drawing on fieldwork in Delhi and Mumbai, this paper explores how police investigations unfolded in the specific context of women’s deaths by burning in India. In particular, it focuses on the use of discretion despite its denial by those exercising it. In India, there are distinctive statutes relating to women’s suspicious deaths, reflecting the widespread expectation that the bride’s family will pay a dowry to the groom’s family and the tensions to which this may on occasion give rise in the early years of a marriage. Often, there are conflicting claims influencing how the woman’s death is classified. These in turn affect police investigation. The nature and direction of police discretion in investigating women’s deaths by burning reflect in part the unique nature of the legislation and the particular sensitivities in relation to these types of death. They also highlight processes that are liable to be at work in any crime investigation. It was found that police officers exercised unacknowledged discretion at seven specific points in the investigative process, with potentially significant consequences for the achievement of just outcomes: first response, recording the victim’s ‘dying declaration’, inquest, registering of the ‘First Information Report’, collecting evidence, arrest and framing of the charges. PMID:26376482

  19. Discrete vs. continuous surface electromyographic interface control.

    PubMed

    Cler, Meredith J; Michener, Carolyn M; Stepp, Cara E

    2014-01-01

    Over 50% of the 273,000 individuals with spinal cord injuries in the US have cervical injuries and are therefore unable to operate a keyboard and mouse with their hands. In this experiment, we compared two systems using surface electromyography (sEMG) recorded from facial muscles to control an onscreen keyboard. Both systems used five sEMG sensors to capture muscle activity during five distinct facial gestures that then mapped to five cursor commands: move left, move right, move up, move down, and click. One system used a discrete movement and feedback algorithm, in which the user would make one quick facial gesture, causing a corresponding discrete movement to an adjacent button. The other system was continuously updated and allowed the user to move in any 360(o) direction smoothly. Information transfer rates (ITRs) in bits per minute were high for both systems. Users of the continuous system showed significantly higher ITRs (average of 68.5; p <; 0.02) compared to users of the discrete system (average of 54.3 bits/min).

  20. Discrete resource allocation in visual working memory

    PubMed Central

    Barton, Brian; Ester, Edward F.; Awh, Edward

    2009-01-01

    Are resources in visual working memory allocated in a continuous or a discrete fashion? On the one hand, flexible resource models suggest that capacity is determined by a central resource pool that can be flexibly divided such that items of greater complexity receive a larger share of resources. On the other hand, if capacity in working memory is defined in terms of discrete storage “slots” then observers may be able to determine which items are assigned to a slot but not how resources are divided between stored items. To test these predictions, we manipulated the relative complexity of the items to be stored while holding the number items constant. Although mnemonic resolution declined when set size increased (Experiment 1), resolution for a given item was unaffected by large variations in the complexity of the other items to be stored when set size was held constant (Experiments 2–4). Thus, resources in visual working memory are distributed in a discrete slot-based fashion, even when inter-item variations in complexity motivate an asymmetrical division of resources across items. PMID:19803642