Impact of Temperature on the Growth and Development of Athetis dissimilis (Lepidoptera: Noctuidae).

PubMed

Guo, Ting-Ting; Li, Li-Li; Men, Xing-Yuan; Lu, Zeng-Bin; Chen, Hao; Wang, Zhen-Ying; Sun, Ting-Lin; Yu, Yi

2017-02-01

Athetis dissimilis (Hampson) has emerged as a serious pest on corn in recent years in China. Understanding the population response of A. dissimilis to temperature will be beneficial for adopting control strategies for this pest. The impact of five constant temperatures (17, 21, 25, 29, and 33 °C) on the life table of A. dissimilis was studied using age-stage, two-sex life table method in the laboratory. The results showed that the developmental time of egg, larva, pupa, and adult decreased when temperature increased from 17 °C to 33 °C. The TPOP (total preoviposition period) decreased with temperature increasing from 17 °C to 29 °C, while the longest APOP (adult preoviposition period) occurred at 21 °C (3.57 d) and the shortest at 33 °C (2.15 d). The fecundity increased from 407.52 to 763.94 eggs as temperatures were raised from 17 to 25 °C, but decreased at temperatures from 25 °C to 33 °C. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R0) increased as temperatures increased from 17 to 25 °C, then decreased when temperatures exceeded 25 °C. In contrast, the mean generation time (T) decreased as temperatures increased from 17 to 33 °C. Based on the estimated data, the highest female age-stage-specific fecundity (fx) and age-specific fecundity (mx) were 81.91 and 45.04 eggs, respectively, at 25 °C. The age-stage life expectancy (exj) of all stages decreased as the temperature increased. The reproductive value (vxj) increased gradually with age and stage. The developmental rates of A. dissimilis between 17 to 29 °C fit the linear equation y  = -0.01315 + 0.001303x, with a coefficient of determination (R2) of 0.9314. In conclusion, our finding clearly states that A. dissimilis has the greatest population increase at 25 °C, and this may help develop appropriate pest management strategies. © The Authors 2016. Published by Oxford University Press on behalf of

Symbiotic cornucopia of the monophagous planthopper Ommatidiotus dissimilis (Fallén, 1806) (Hemiptera: Fulgoromorpha: Caliscelidae).

PubMed

Michalik, Anna; Szwedo, Jacek; Stroiński, Adam; Świerczewski, Dariusz; Szklarzewicz, Teresa

2018-03-07

In contrast to Cicadomorpha, in which numerous symbiotic bacteria have been identified and characterized, the symbionts of fulgoromorphans are poorly known. Here, we present the results of histological, ultrastructural, and molecular analyses of the symbiotic system of the planthopper Ommatidiotus dissimilis. Amplification, cloning, and sequencing of bacterial 16S RNA genes have revealed that O. dissimilis is host to five types of bacteria. Apart from bacteria Sulcia and Vidania, which are regarded as ancestral symbionts of Fulgoromorpha, three additional types of bacteria belonging to the genera Sodalis, Wolbachia, and Rickettsia have been detected. Histological and ultrastructural investigations have shown that bacteria Sulcia, Vidania, and Sodalis house separate bacteriocytes, whereas bacteria Wolbachia and Rickettsia are dispersed within various insect tissue. Additionally, bacteria belonging to the genus Vidania occupy the bacteriome localized in the lumen of the hindgut. Both molecular and microscopic analyses have revealed that all the symbionts are transovarially transmitted between generations.

Evidence of morphine like substance and μ-opioid receptor expression in Toxacara canis (Nematoda: Ascaridae)

PubMed Central

Golabi, Mostafa; Naem, Soraya; Imani, Mehdi; Dalirezh, Nowruz

2016-01-01

Toxocara canis (Nematoda: Ascaridae) is an intestinal nematode parasite of dogs, which can also cause disease in humans. Transmission to humans usually occurs because of direct contact with T. canis eggs present in soil contaminated with the feces of infected dogs. This nematode has extraordinary abilities to survive for many years in different tissues of vertebrates, and develop to maturity in the intestinal tract of its definitive host. Survival of parasitic nematodes within a host requires immune evasion using complicated pathways. Morphine-like substance, as well as opioids, which are known as down regulating agents, can modulate both innate and acquired immune responses, and let the parasite survives in their hosts. In the present study, we aimed to find evidences of morphine-like substance and µ-opiate receptor expression in T. canis, using high performance liquid chromatography (HPLC) and reverse transcription polymerase chain reaction (RT-PCR). The results indicated that T. canis produced morphine-like substances at the level of 2.31± 0.26 ng g-1 wet weight, and expressed µ-opiate receptor as in expected size of 441 bp. According to our findings, it was concluded that T. canis, benefits using morphine-like substance to modulate host immunity. PMID:28144426

Evidence of morphine like substance and μ-opioid receptor expression in Toxacara canis (Nematoda: Ascaridae).

PubMed

Golabi, Mostafa; Naem, Soraya; Imani, Mehdi; Dalirezh, Nowruz

2016-01-01

Toxocara canis (Nematoda: Ascaridae) is an intestinal nematode parasite of dogs, which can also cause disease in humans. Transmission to humans usually occurs because of direct contact with T. canis eggs present in soil contaminated with the feces of infected dogs. This nematode has extraordinary abilities to survive for many years in different tissues of vertebrates, and develop to maturity in the intestinal tract of its definitive host. Survival of parasitic nematodes within a host requires immune evasion using complicated pathways. Morphine-like substance, as well as opioids, which are known as down regulating agents, can modulate both innate and acquired immune responses, and let the parasite survives in their hosts. In the present study, we aimed to find evidences of morphine-like substance and µ-opiate receptor expression in T. canis , using high performance liquid chromatography (HPLC) and reverse transcription polymerase chain reaction (RT-PCR). The results indicated that T. canis produced morphine-like substances at the level of 2.31± 0.26 ng g -1 wet weight, and expressed µ-opiate receptor as in expected size of 441 bp. According to our findings, it was concluded that T. canis , benefits using morphine-like substance to modulate host immunity.

Identification of Putative Chemosensory Receptor Genes from the Athetis dissimilis Antennal Transcriptome

PubMed Central

Dong, Junfeng; Song, Yueqin; Li, Wenliang; Shi, Jie; Wang, Zhenying

2016-01-01

Olfaction plays a crucial role in insect population survival and reproduction. Identification of the genes associated with the olfactory system, without the doubt will promote studying the insect chemical communication system. In this study, RNA-seq technology was used to sequence the antennae transcriptome of Athetis dissimilis, an emerging crop pest in China with limited genomic information, with the purpose of identifying the gene set involved in olfactory recognition. Analysis of the transcriptome of female and male antennae generated 13.74 Gb clean reads in total from which 98,001 unigenes were assembled, and 25,930 unigenes were annotated. Total of 60 olfactory receptors (ORs), 18 gustatory receptors (GRs), and 12 ionotropic receptors (IRs) were identified by Blast and sequence similarity analyzes. One obligated olfactory receptor co-receptor (Orco) and four conserved sex pheromone receptors (PRs) were annotated in 60 ORs. Among the putative GRs, five genes (AdisGR1, 6, 7, 8 and 94) clustered in the sugar receptor family, and two genes (AdisGR3 and 93) involved in CO2 detection were identified. Finally, AdisIR8a.1 and AdisIR8a.2 co-receptors were identified in the group of candidate IRs. Furthermore, expression levels of these chemosensory receptor genes in female and male antennae were analyzed by mapping the Illumina reads. PMID:26812239

The mitochondrial genomes of the human hookworms, Ancylostoma duodenale and Necator americanus (Nematoda: Secernentea).

PubMed

Hu, Min; Chilton, Neil B; Gasser, Robin B

2002-02-01

The complete mitochondrial genome sequences were determined for two species of human hookworms, Ancylostoma duodenale (13,721 bp) and Necator americanus (13,604 bp). The circular hookworm genomes are amongst the smallest reported to date for any metazoan organism. Their relatively small size relates mainly to a reduced length in the AT-rich region. Both hookworm genomes encode 12 protein, two ribosomal RNA and 22 transfer RNA genes, but lack the ATP synthetase subunit 8 gene, which is consistent with three other species of Secernentea studied to date. All genes are transcribed in the same direction and have a nucleotide composition high in A and T, but low in G and C. The AT bias had a significant effect on both the codon usage pattern and amino acid composition of proteins. For both hookworm species, genes were arranged in the same order as for Caenorhabditis elegans, except for the presence of a non-coding region between genes nad3 and nad5. In A. duodenale, this non-coding region is predicted to form a stem-and-loop structure which is not present in N. americanus. The mitochondrial genome structure for both hookworms differs from Ascaris suum only in the location of the AT-rich region, whereas there are substantial differences when compared with Onchocerca volvulus, including four gene or gene-block translocations and the positions of some transfer RNA genes and the AT-rich region. Based on genome organisation and amino acid sequence identity, A. duodenale and N. americanus were more closely related to C. elegans than to A. suum or O. volvulus (all secernentean nematodes), consistent with a previous phylogenetic study using ribosomal DNA sequence data. Determination of the complete mitochondrial genome sequences for two human hookworms (the first members of the order Strongylida ever sequenced) provides a foundation for studying the systematics, population genetics and ecology of these and other nematodes of socio-economic importance.

Ultrastructure of the post-corpus of Zeldia punctata (Cephalobina) for analysis of the evolutionary framework of nematodes related to Caenorhabditis elegans (Rhabditina).

PubMed Central

Zhang, Y C; Baldwin, J G

2000-01-01

The ultrastructure of the post-corpus of Zeldia punctata (Cephalobina) was compared with previous observations of Caenorhabditis elegans (Rhabditina) and Diplenteron sp. (Diplogastrina) with the goal of interpreting the morphological evolution of the feeding structures in the Secernentea. The post-corpus of Z. punctata consists of six marginal, 13 muscle, five gland and seven nerve cells. The most anterior of four layers of muscle cells consists of six mononucleate cells in Z. punctata. The homologous layer in C. elegans and Diplenteron consists of three binucleate cells, suggesting a unique derived character (synapomorphy) shared between the Rhabditina and Diplogastrina. Contrary to Diplenteron sp. where we observed three oesophageal glands, Z. punctata and C. elegans have five oesophageal glands. We question this shared character as reflecting a common evolution between the Cephalobina and Rhabditina, because there are strong arguments for functional (adaptive) convergence of the five glands in these bacterial feeders. Convergence is further suggested by the mosaic distribution of three versus five glands throughout the Nemata; this distribution creates difficulties in establishing character polarity. Although morphological data are often laborious to recover and interpret, we nevertheless view 'reciprocal illumination' between molecular and morphological characters as the most promising and robust process for reconstructing the evolution of the Secernentea and its feeding structures. PMID:10902689

New species and new records of deepwater munidid squat lobsters from north-western Australia: Onconida, Bathymunida, Crosnierita, Plesionida and Torbenella.

PubMed

Ahyong, Shane T; Taylor, Joanne; Mccallum, Anna W

2013-11-04

Seven species of Munididae are reported from the continental margin of north-western Australia. Three species are new to science: Crosnierita adela sp. nov., Onconida ariel sp. nov. and Plesionida aurelia sp. nov., each presently known only from Western Australia. Four species are reported for the first time from Australian waters, Bathymunida balssi Van Dam, 1838, Bathymunida dissimilis Baba & de Saint Laurent, 1996, Crosnierita yante (Macpherson, 1994) and Torbenella orbis (Baba, 2005). Keys to the world species of the genera represented are provided.

New record of Ascaridia nymphii (Secernentea: Ascaridiidae) from macaw parrot, Ara chloroptera, in China.

PubMed

Yang, Fang; Zhang, Pan; Shi, Xianli; Li, Kangxin; Wang, Minwei; Fu, Yeqi; Yan, Xinxin; Hang, Jianxiong; Li, Guoqing

2018-06-01

Present study was performed to identify the species of ascarids from macaw parrot, Ara chloroptera, in China. Total 6 ascarids (3 males and 3 females) were collected in the feces of 3 macaws at Guangzhou Zoo in Guangdong Province, China. Their morphological characteristics with dimensions were observed under a light microscope, and their genetic characters were analyzed with the partial 18S rDNA, ITS rDNA and nad4 gene sequences, respectively. Results showed that all worms have no interlabia but male worms have two alate spicules, well-developed precloacal sucker and a tail with ventrolateral caudal alae and 11 pairs of papillae. The partial 18S rDNA, ITS rDNA and nad4 sequences were 831bp, 1015bp and 394bp in length, respectively. They showed the highest similarity of 99.8% (18S rDNA) with Ascaridia nymphii, 93.8% identities (ITS rDNA) with A. columbae and 98.5% to 99.5% identities (nad4) with Ascaridia sp. from infected parrot. All Ascaridia nematodes from the macaws were clustered into one clade and formed monophyletic group of Ascaridia with A. columbae and A. galli in two phylogenetic trees. It is observed that the combining morphological and sequencing data from three loci, the present Ascaridia species was identified as Ascaridia nymphii, which is the first record of A. nymphii from macaw parrot in China. Copyright © 2018 Elsevier B.V. All rights reserved.

Revision of Tomocerus similis Chen & Ma, with discussion of the kinoshitai complex and the distal tibiotarsal chaetae in Tomocerinae (Collembola, Tomoceridae).

PubMed

Yu, Daoyuan; Ding, Yinhuan; Ma, Yitong

2017-05-17

Molecular analysis and a detailed morphological comparison revealed that Tomocerus similis Chen & Ma was described from individuals belonging to several species from several localities. Based on both old and new material from Anhui and Jiangsu Provinces, China, T. similis is redescribed and two new species are described. The three species are morphologically similar. Tomocerus persimilis sp. nov. differs from the others by the presence of central macrochaeta on head and of several distinct distal inner teeth on unguis. Tomocerus dissimilis sp. nov. is characterised by pointed tenent hairs on anterior legs. Remarks are made on the systematics and ecology of the kinoshitai complex, and on the taxonomic value of tenent hair and its adjacent chaetae.

First report of parasitism by Hexametra boddaertii (Nematoda: Ascaridae) in Oxyrhopus guibei (Serpentes: Colubridae).

PubMed

Peichoto, María E; Sánchez, Matías N; López, Ariel; Salas, Martín; Rivero, María R; Teibler, Pamela; Toledo, Gislayne de Melo; Tavares, Flávio L

2016-07-15

The current study summarizes the postmortem examination of a specimen of Oxyrhopus guibei (Serpentes, Colubridae) collected in Iguazu National Park (Argentina), and found deceased a week following arrival to the serpentarium of the National Institute of Tropical Medicine (Argentina). Although the snake appeared to be in good health, a necropsy performed following its death identified the presence of a large number of roundworms in the coelomic cavity, with indications of peritonitis and serosal adherence. Additional observations from the necropsy revealed small calcifications in the mesothelium of the coelomic cavity; solid and expressive content in the gallbladder; massive gastrointestinal obstruction due to nematodes; and lung edema and congestion. Histopathological analyses of lung sections also showed proliferative heterophilic and histiocytic pneumonia. Parasites isolated from both the intestine and coelomic cavity were identified as Hexametra boddaertii by a combination of light and scanning electron microscopic examination. Results from this necropsy identify O. guibei as a new host for H. boddaertii, and is the first report of a natural infection by Hexametra in Argentina. Since Hexametra parasites may contribute to several pathological conditions in humans, and with the recent availability of O. guibei specimens through the illegal pet trade, it is necessary to consider the possibility of zoonotic helminth transmission of Hexametra from snake to human. Copyright © 2016 Elsevier B.V. All rights reserved.

Taxonomic notes on Holcobunus Roewer, 1910, with descriptions of three new species, and new records for Holcobunus nigripalpis Roewer, 1910 (Opiliones: Eupnoi: Sclerosomatidae).

PubMed

Tourinho, Ana Lúcia; Pinto-da-Rocha, Ricardo; Bragagnolo, Cibele

2015-10-05

Three new Brazilian species of Holcobunus Roewer, 1910 are described, thus increasing the total number of species in the genus to five: Holcobunus bicornutus Mello-Leitão, 1940, H. nigripalpis Roewer, 1910, Holcobunus dissimilis sp. nov. (type locality: Espírito Santo, Santa Teresa, Reserva Biologia Augusto Ruschi), Holcobunus ibitirama sp. nov. (type locality: Espírito Santo, Ibitirama, Santa Marta, close to Parque Nacional Caparaó), and Holcobunus uaisoh sp. nov. (type locality: Minas Gerais, Fervedouro, Parque Estadual Serra do Brigadeiro). A new record for Holcobunus nigripalpis Roewer, 1910 from Minas Gerais is also provided and the morphological variation in both penis and somatic morphology in the genus are presented and discussed. These observations enhance our understanding of both the diversity and distribution of Holcobunus.

Nematodes as Sentinels of Heavy Metals and Organic Toxicants in the Soil

PubMed Central

Ekschmitt, Klemens; Korthals, Gerard W.

2006-01-01

Field and laboratory research has repeatedly shown that free-living soil nematodes differ in their sensitivity to soil pollution. In this paper, we analyze whether nematode genera proved sensitive or tolerant toward heavy metals and organic pollutants in six long-term field experiments. We discuss overlaps between nematode physiological responses to heavy metals and to organic pollutants, which may explain why nematodes can exhibit co-tolerance toward several contaminants. We propose a simple method for separating direct effects of soil contamination on nematode populations from indirect effects mediated through the food chain. Finally, we analyze the extent to which nematodes exhibited consistent responses across the experiments analyzed. Our results show that (a) indirect effects of pollution were generally strong; (b) fewer nematode genera were tolerant than sensitive; (c) many genera, including practically all Adenophorea, exhibited a common response pattern to contaminants; and (d) several genera of the Secernentea exhibited differential tolerance toward particular pollutants. We conclude that bioindication of soil contamination should preferentially be based on tolerant, and less on sensitive, nematodes. We provide a list of nematode genera that may potentially serve as differential bioindicators for specific soil contaminants. PMID:19259425

New data on the taxonomy and distribution of ten Neotropical chewing lice of the genus Myrsidea (Phthiraptera: Menoponidae), including the description of a new species.

PubMed

Kolencik, Stanislav; Sychra, Oldrich; Valan, Miroslav; Literak, Ivan

2016-03-02

The new species Myrsidea alexanderi is described and illustrated ex Pheugopedius maculipectus (Troglodytidae) from Honduras. Redescriptions and illustrations are given for both sexes of Myrsidea chiapensis ex Calocitta formosa from Costa Rica, and the male of M. dissimilis ex Progne chalybea from Brazil. Also, seven other previously known species or subspecies of the louse genus Myrsidea are recorded and discussed from passerine birds of the Neotropical Region, as follows: Myrsidea antiqua, Myrsidea balteri, Myrsidea diffusa, Myrsidea nesomimi borealis, Myrsidea paleno, Myrsidea psittaci and Myrsidea serini. Our data increase knowledge of intraspecific morphological variability within these species, and also of their host and geographical distribution. New host-louse associations are: Agelaioides badius for M. psittaci; Basileuterus culicivorus and Myiothlypis leucoblephara for M. paleno; Mimus saturninus for M. nesomimi borealis; and Icterus dominicensis and Molothrus rufoaxillaris for Myrsidea sp.

Mitochondrial DNA Sequence Divergence among Meloidogyne incognita, Romanomermis culicivorax, Ascaris suum, and Caenorhabditis elegans

PubMed Central

Powers, T. O.; Harris, T. S.; Hyman, B. C.

1993-01-01

Mitochondrial DNA sequences were obtained from the NADH dehydrogenase subunit 3 (ND3), large rRNA, and cytochrome b genes from Meloidogyne incognita and Romanomermis culicivorax. Both species show considerable genetic distance within these same genes when compared with Caenorhabditis elegans or Ascaris suum, two species previously analyzed. Caenorhabditis, Ascaris, and Meloidogyne were selected as representatives of three subclasses in the nematode class Secernentea: Rhabditia, Spiruria, and Diplogasteria, respectively. Romanomermis served as a representative out-group of the class Adenophorea. The divergence between the phytoparasitic lineage (represented by Meloidogyne) and the three other species is so great that virtually every variable position in these genes appears to have accumulated multiple mutations, obscuring the phylogenetic information obtainable from these comparisons. The 39 and 42% amino acid similarity between the M. incognita and C. elegans ND3 and cytochrome b coding sequences, respectively, are approximately the same as those of C. elegans-mouse comparisons for the same genes (26 and 44%). This discovery calls into question the feasibility of employing cloned C. elegans probes as reagents to isolate phytoparasitic nematode genes. The genetic distance between the phytoparasitic nematode lineage and C. elegans markedly contrasts with the 79% amino acid similarity between C. elegans and A. suum for the same sequences. The molecular data suggest that Caenorhabditis and Ascaris belong to the same subclass. PMID:19279810

Palynology of the Heath Formation (Miocene) from the Progreso Basin, Peru

DOE Office of Scientific and Technical Information (OSTI.GOV)

Engelhardt, D.W.; Wood, G.D.

1993-02-01

A diverse and well preserved assemblage of pollen, spores, dinoflagellates, and acritarchs were recovered from outcrop samples of the Heath Formation, exposed along Bocapan Creek near Tumbes, Peru. The pollen and spores include representatives of Arecipites, Bombacacidites, Caryapollenites, Cicatricosisporites, Couperipollis, Cyathidites, Diporisporites, Distaverrusporites, Dyadosporites, Echiperiporites, Faramea, Foveodiporites, Foveotriletes, Fusiformisporites, Gothanipollis, Granitricolpites, Hymenophyllum, Hexpollenties, Involutisporites, Laevigatosporites, Lygodiumsporites, Magniperiporites, Malvacearumpollis, Monocolpopollenites, Perissyncolporites, Peritheciumites, Phragmothyrites, Polyadadporites, Polypodiaceisporites, Polypodiisporites, Retibrevitricolpites, Striadisporites, Tetracolporites, Tricolporopollenties, and Verrucosisporites. Plankton are assignable to Lejeunecysta, Operculodinium, Pterospermella, Selenopemphix, Spiniferites, Sumatradinium, Tythodiscus, and Tuberculodinium. The palynomorph assemblage can be placed in the Early Miocene based on the co-occurrence of Cicatricosisporitesmore » dorogensis, Couperipollis rarispinosus, Echiperiporites estelae, Magniperiporites echinatus, Perisyncolporites porkornii, Polypodiaceoisporites minor, P. potoniei, Reticolporites guianesnsis, R. irregularis, Scabriporites asymetricus, Selenopemphix nephroides and Tuberculodinium vancampoae. This is an agreement with foraminiferal evidence which positions the Heath Formation in the Early Miocene Catapsydrax dissimilis, Catapsydrax stainforthi and oldest portion of the Globigerinatella insueta zones.« less

Cirolana bambang, a distinctive new species of Cirolana Leach, 1818 (Crustacea: Isopoda: Cirolanidae) from Bitung, Indonesia.

PubMed

Sidabalok, Conni M; Bruce, Niel L

2018-01-25

Cirolana bambang sp. nov. from Bitung, North Sulawesi, Indonesia is described. The species can be recognised by generally smooth body with small, acute, submedian nodules on pleonites 4-5 and the anterior dorsal surface of the pleotelson together with a flat, pentagonal frontal lamina, linguiform and sexually dimorphic pleotelson with the dorsal surface covered by short setae in males, deeply bifid uropod apices, and a conspicuously large robust seta on the superodistal angle of pereopod 1 ischium. Cirolana bambang sp. nov. appears to belong to a group of Indo-West Pacific Cirolana with prominent penial process, a large robust seta on the superodistal angle of pereopod 1 ischium and the presence of setae on dorsal surface of pleotelson and or uropod in males. The most similar species are Cirolana comata Keable, 2001; Cirolana dissimilis Keable, 2001; Cirolana aldabrensis Schotte Kensley, 2005; and Cirolana somalia Schotte Kensley, 2005. Cirolana kiliani Müller, 1993 and C. somalia are found not to belong to the Cirolana'parva-group'.

DNA barcoding as an aid for species identification in austral black flies (Insecta: Diptera: Simuliidae).

PubMed

Hernández-Triana, Luis M; Montes De Oca, Fernanda; Prosser, Sean W J; Hebert, Paul D N; Gregory, T Ryan; McMurtrie, Shelley

2017-04-01

In this paper, the utility of a partial sequence of the COI gene, the DNA barcoding region, for the identification of species of black flies in the austral region was assessed. Twenty-eight morphospecies were analyzed: eight of the genus Austrosimulium (four species in the subgenus Austrosimulium s. str., three species in the subgenus Novaustrosimulium, and one species unassigned to subgenus), two of the genus Cnesia, eight of Gigantodax, three of Paracnephia, one of Paraustrosimulium, and six of Simulium (subgenera Morops, Nevermannia, and Pternaspatha). The neighbour-joining tree derived from the DNA barcode sequences grouped most specimens according to species or species groups recognized by morphotaxonomic studies. Intraspecific sequence divergences within morphologically distinct species ranged from 0% to 1.8%, while higher divergences (2%-4.2%) in certain species suggested the presence of cryptic diversity. The existence of well-defined groups within S. simile revealed the likely inclusion of cryptic diversity. DNA barcodes also showed that specimens identified as C. dissimilis, C. nr. pussilla, and C. ornata might be conspecific, suggesting possible synonymy. DNA barcoding combined with a sound morphotaxonomic framework would provide an effective approach for the identification of black flies in the region.

Cumberlandian Mollusk Conservation Program. Activity 3: identification of fish hosts. [Conradilla caelata; Quadrula intermedia; Epioblasma brevidens; Epioblasma capsaeformis; Epioblasma triquetra; Quadrula cylindrica; Carunculina moesta

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hill, D.M.

A key element of the Cumberlandian Mollusk Conservation Program undertaken by TVA in 1979 was the determination of fish hosts of Cumberlandian mussel species unique to the Tennessee River drainage and especially the species whose habitat would be inundated by completion of Columbia Dam on the Duck River, Tennessee. Principal emphasis was placed on the birdwing pearly mussel, Conradilla caelata and the Cumberland monkeyface, Quadrula intermedia - two federally listed endangered species with limited distributions outside the proposed inundation zone of the Duck River. Additional species studied included three species of the genus Epioblasma (E. brevidens, E. capsaeformis, and E.more » triquetra), Quadrula cylindrica, Villosa iris, and Carunculina moesta. Experimental glochidial infection of 55 fish species resulted in the establishment of the following mussel-fish host relationships: Conradilla caelata - Etheostoma zonale; Quadrule intermedia - Hybopsis dissimilis, Hybopsis insignis; Epioblasma brevidens - Etheostoma blennioides, Etheostoma maculatum, Etheostoma rufilineatum, Etheostoma simoterum, Percina caprodes, Cottus carolinae; Epioblasma capsaeformis - Etheostoma maculatum, Etheostoma rufilineatum, Percina sciera, Cottus carolinae; Epioblasma triquetra - Percina caprodes, Cottus carolinae; Quadrula cylindrica - Notropis galacturus, Notropis spilopterus, Hybopsis amblops; and Carunculina moesta - Lepomis cyanellus, Lepomis megalotis.« less

The genus Rosalba Thomson, 1864 (Coleoptera, Cerambycidae, Lamiinae, Apomecynini).

PubMed

Santos-Silva, Antonio; Galileo, Maria Helena M; Joly, Luis J; Tavakilian, GÉrard L

2018-02-26

Nineteen new species are described: Rosalba wappesi, from Bolivia; R. giesberti, from Bolivia; R. skillmani, from Bolivia; R. lingafelteri, from Bolivia; R. senecauxi, from French Guiana; R. dalensi, from French Guiana; R. giuglarisi, from French Guiana; R. nearnsi, from French Guiana; R. cerdai, from French Guiana; R. gaianii, from Venezuela; R. pittieri, from Venezuela; R. clinei, from Bolivia and Brazil; R. morrisi, from Costa Rica and Panama; R. schneppi, from Panama; R. birai, from Colombia; R. stenodesma, from Venezuela and French Guiana; R. similis, from Peru and Ecuador; R. bezarki, from Ecuador; and R.seraisorum, from Ecuador and Brazil (Amazonas). The following new synonymies are established: Aletretia dissimilis Belon, 1903 = A. fimbriata Belon, 1903; Aletretia consobrina Melzer, 1934, Rosalba gounellei Galileo Martins, 2013 and Rosalba vanini Galileo Martins, 2013 = Aletretia inscripta Bates, 1866; Aletretia mediofasciata Breuning, 1943 = Aletretia bucki Melzer, 1934; Aletretia peraffinis Breuning, 1940 = Aletretia approximata Melzer, 1934. The following species are redescribed: Rosalba strandiella (Breuning, 1940); and Rosalba rufescens (Breuning, 1940). The following new records are reported: Rosalba strandiella for Bolivia; R. strandi (Breuning, 1943) from the Brazilian states of São Paulo, Paraná and Santa Catarina; R. inscripta from Peru and the Brazilian state of Acre; R. smaragdina (Breuning, 1940) from the Brazilian state of Minas Gerais; R. obliqua (Thomson, 1868) from Brazil; and Rosalba fimbriata (Belon, 1903) from Ecuador.