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Sample records for dissimilis secernentea ascarida

  1. Strongyloides myopotami (Secernentea: Strongyloididae) from the intestine of feral nutrias (Myocastor coypus) in Korea.

    PubMed

    Choe, Seongjun; Lee, Dongmin; Park, Hansol; Oh, Mihyeon; Jeon, Hyeong-Kyu; Eom, Keeseon S

    2014-10-01

    Surveys on helminthic fauna of the nutria, Myocastor coypus, have seldom been performed in the Republic of Korea. In the present study, we describe Strongyloides myopotami (Secernentea: Strongyloididae) recovered from the small intestine of feral nutrias. Total 10 adult nutrias were captured in a wetland area in Gimhae-si (City), Gyeongsangnam-do (Province) in April 2013. They were transported to our laboratory, euthanized with ether, and necropsied. About 1,300 nematode specimens were recovered from 10 nutrias, and some of them were morphologically observed by light and scanning electron microscopies. They were 3.7-4.7 (4.0±0.36) mm in length, 0.03-0.04 (0.033) mm in width. The worm dimension and other morphological characters, including prominent lips of the vulva, blunted conical tail, straight type of the ovary, and 8-chambered stoma, were all consistent with S. myopotami. This nematode fauna is reported for the first time in Korea.

  2. Evidence of morphine like substance and μ-opioid receptor expression in Toxacara canis (Nematoda: Ascaridae)

    PubMed Central

    Golabi, Mostafa; Naem, Soraya; Imani, Mehdi; Dalirezh, Nowruz

    2016-01-01

    Toxocara canis (Nematoda: Ascaridae) is an intestinal nematode parasite of dogs, which can also cause disease in humans. Transmission to humans usually occurs because of direct contact with T. canis eggs present in soil contaminated with the feces of infected dogs. This nematode has extraordinary abilities to survive for many years in different tissues of vertebrates, and develop to maturity in the intestinal tract of its definitive host. Survival of parasitic nematodes within a host requires immune evasion using complicated pathways. Morphine-like substance, as well as opioids, which are known as down regulating agents, can modulate both innate and acquired immune responses, and let the parasite survives in their hosts. In the present study, we aimed to find evidences of morphine-like substance and µ-opiate receptor expression in T. canis, using high performance liquid chromatography (HPLC) and reverse transcription polymerase chain reaction (RT-PCR). The results indicated that T. canis produced morphine-like substances at the level of 2.31± 0.26 ng g-1 wet weight, and expressed µ-opiate receptor as in expected size of 441 bp. According to our findings, it was concluded that T. canis, benefits using morphine-like substance to modulate host immunity. PMID:28144426

  3. Evidence of morphine like substance and μ-opioid receptor expression in Toxacara canis (Nematoda: Ascaridae).

    PubMed

    Golabi, Mostafa; Naem, Soraya; Imani, Mehdi; Dalirezh, Nowruz

    2016-01-01

    Toxocara canis (Nematoda: Ascaridae) is an intestinal nematode parasite of dogs, which can also cause disease in humans. Transmission to humans usually occurs because of direct contact with T. canis eggs present in soil contaminated with the feces of infected dogs. This nematode has extraordinary abilities to survive for many years in different tissues of vertebrates, and develop to maturity in the intestinal tract of its definitive host. Survival of parasitic nematodes within a host requires immune evasion using complicated pathways. Morphine-like substance, as well as opioids, which are known as down regulating agents, can modulate both innate and acquired immune responses, and let the parasite survives in their hosts. In the present study, we aimed to find evidences of morphine-like substance and µ-opiate receptor expression in T. canis, using high performance liquid chromatography (HPLC) and reverse transcription polymerase chain reaction (RT-PCR). The results indicated that T. canis produced morphine-like substances at the level of 2.31± 0.26 ng g(-1) wet weight, and expressed µ-opiate receptor as in expected size of 441 bp. According to our findings, it was concluded that T. canis, benefits using morphine-like substance to modulate host immunity.

  4. Impact of Temperature on the Growth and Development of Athetis dissimilis (Lepidoptera: Noctuidae).

    PubMed

    Guo, Ting-Ting; Li, Li-Li; Men, Xing-Yuan; Lu, Zeng-Bin; Chen, Hao; Wang, Zhen-Ying; Sun, Ting-Lin; Yu, Yi

    2017-02-01

    Athetis dissimilis (Hampson) has emerged as a serious pest on corn in recent years in China. Understanding the population response of A. dissimilis to temperature will be beneficial for adopting control strategies for this pest. The impact of five constant temperatures (17, 21, 25, 29, and 33 °C) on the life table of A. dissimilis was studied using age-stage, two-sex life table method in the laboratory. The results showed that the developmental time of egg, larva, pupa, and adult decreased when temperature increased from 17 °C to 33 °C. The TPOP (total preoviposition period) decreased with temperature increasing from 17 °C to 29 °C, while the longest APOP (adult preoviposition period) occurred at 21 °C (3.57 d) and the shortest at 33 °C (2.15 d). The fecundity increased from 407.52 to 763.94 eggs as temperatures were raised from 17 to 25 °C, but decreased at temperatures from 25 °C to 33 °C. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R0) increased as temperatures increased from 17 to 25 °C, then decreased when temperatures exceeded 25 °C. In contrast, the mean generation time (T) decreased as temperatures increased from 17 to 33 °C. Based on the estimated data, the highest female age-stage-specific fecundity (fx) and age-specific fecundity (mx) were 81.91 and 45.04 eggs, respectively, at 25 °C. The age-stage life expectancy (exj) of all stages decreased as the temperature increased. The reproductive value (vxj) increased gradually with age and stage. The developmental rates of A. dissimilis between 17 to 29 °C fit the linear equation y  = -0.01315 + 0.001303x, with a coefficient of determination (R2) of 0.9314. In conclusion, our finding clearly states that A. dissimilis has the greatest population increase at 25 °C, and this may help develop appropriate pest management strategies. © The Authors 2016. Published by Oxford University Press on behalf of

  5. Strongyloides myopotami (Secernentea: Strongyloididae) from the Intestine of Feral Nutrias (Myocastor coypus) in Korea

    PubMed Central

    Choe, Seongjun; Lee, Dongmin; Park, Hansol; Oh, Mihyeon; Jeon, Hyeong-Kyu

    2014-01-01

    Surveys on helminthic fauna of the nutria, Myocastor coypus, have seldom been performed in the Republic of Korea. In the present study, we describe Strongyloides myopotami (Secernentea: Strongyloididae) recovered from the small intestine of feral nutrias. Total 10 adult nutrias were captured in a wetland area in Gimhae-si (City), Gyeongsangnam-do (Province) in April 2013. They were transported to our laboratory, euthanized with ether, and necropsied. About 1,300 nematode specimens were recovered from 10 nutrias, and some of them were morphologically observed by light and scanning electron microscopies. They were 3.7-4.7 (4.0±0.36) mm in length, 0.03-0.04 (0.033) mm in width. The worm dimension and other morphological characters, including prominent lips of the vulva, blunted conical tail, straight type of the ovary, and 8-chambered stoma, were all consistent with S. myopotami. This nematode fauna is reported for the first time in Korea. PMID:25352703

  6. Comparative cytological studies of mitotic and male meiotic karyotype of Ascaridia dissimilis (Vigueras, 1931) and Ascaridia galli (Schrank, 1788).

    PubMed

    Mutafova, T

    1976-02-06

    Gametogony and spermatogenesis of A. dissimilis and A. galli were studied. It was found that the chromosome number of A. dissimilis is 2n = 10 for female specimens and 2n = 9 for male ones. The chromosome number of A. galli is also 2n = 10 for female specimens and 2n = 9 for males ones. Comparison of the relative length of chromosomes in the chromosome set, expressed in percentages, shows nearly equal magnitudes for both species. Spermatogenesis processes were studied. It was found that they run a similar course in both species. In the following stages of gametogony and spermatogenesis differences between the two species are not discovered.

  7. Oviposition of Tanytarsus dissimilis (Diptera:Chironomidae) in avoidance trails with coal liquid water-soluble components

    SciTech Connect

    Dauble, D.D.; Skalski, J.R.

    1983-12-01

    Oviposition site preference (OSP) of the chironomid Tanytarsus dissimilis (Johannsen) was evaluated in avoidance trials with acutely toxic concentrations of a coal liquid water-soluble fraction (WSF). Tests conducted with groups and with single organisms indicated that ovipositing adults had no significant preference (..cap alpha.. = 0.05) for either river water (control) or a coal liquid WSF. Egg strand size was reduced in the coal liquid WSF, suggesting that toxicant detection occurred despite lack of avoidance. The OSP trials conducted with single organisms were advantageous because of lack of independence in group tests and because greater sample size could be obtained with less effort. This type of behavioral study may have an application to hazard evaluation of other toxic substances.

  8. First report of parasitism by Hexametra boddaertii (Nematoda: Ascaridae) in Oxyrhopus guibei (Serpentes: Colubridae).

    PubMed

    Peichoto, María E; Sánchez, Matías N; López, Ariel; Salas, Martín; Rivero, María R; Teibler, Pamela; Toledo, Gislayne de Melo; Tavares, Flávio L

    2016-07-15

    The current study summarizes the postmortem examination of a specimen of Oxyrhopus guibei (Serpentes, Colubridae) collected in Iguazu National Park (Argentina), and found deceased a week following arrival to the serpentarium of the National Institute of Tropical Medicine (Argentina). Although the snake appeared to be in good health, a necropsy performed following its death identified the presence of a large number of roundworms in the coelomic cavity, with indications of peritonitis and serosal adherence. Additional observations from the necropsy revealed small calcifications in the mesothelium of the coelomic cavity; solid and expressive content in the gallbladder; massive gastrointestinal obstruction due to nematodes; and lung edema and congestion. Histopathological analyses of lung sections also showed proliferative heterophilic and histiocytic pneumonia. Parasites isolated from both the intestine and coelomic cavity were identified as Hexametra boddaertii by a combination of light and scanning electron microscopic examination. Results from this necropsy identify O. guibei as a new host for H. boddaertii, and is the first report of a natural infection by Hexametra in Argentina. Since Hexametra parasites may contribute to several pathological conditions in humans, and with the recent availability of O. guibei specimens through the illegal pet trade, it is necessary to consider the possibility of zoonotic helminth transmission of Hexametra from snake to human.

  9. Comparative and experimental embryogenesis of Plectidae (Nematoda).

    PubMed

    Lahl, Vera; Halama, Christian; Schierenberg, Einhard

    2003-02-01

    Comparative analysis of early embryogenesis indicates that considerable differences exist among nematode species. To better understand to what extent the well-studied development of Caenorhabditis elegans is representative for nematodes in general, we extended our earlier studies to other families of this phylum. Here we report our findings on seven species of Plectidae. We found that Plectidae embryos share a number of developmental similarities with one branch of nematodes (Secernentea), including C. elegans, but not with the other branch (Adenophorea), and thus support conclusions concerning their phylogenetic position drawn from molecular data. However, Plectidae also show developmental differences to other Secernentea, suggesting an early separation from them. Prominent characteristics of Plectidae are (1) strict left-right divisions of somatic founder cells generating a prominent early bilateral symmetry and (2) a very early start of gastrulation with immigration of a single gut precursor cell. To determine whether gastrulation with two gut precursors is crucial for C. elegans embryos, we induced it to gastrulate with a single blastomere like in Plectidae. As this alteration is compatible with an essentially normal subsequent embryogenesis, cleavage of the gut precursor before gastrulation is obviously not required. As major differences exist among nematodes concerning the potential to compensate for eliminated early blastomeres, we tested this feature in one Plectus species. We found that Plectus does not replace a lost cell but behaves like C. elegansin this respect, in contrast to our previous findings in Acrobeloides nanus, another member of the Secernentea.

  10. Toxicity of TNT Wastewaters to Aquatic Organisms. Volume 2. Acute Toxicity of Condensate Wastewater and 2,4-Dinitrotoluene

    DTIC Science & Technology

    1983-03-01

    magna), scud (Hyalella azteca), and worm ( Lumbriculus variegatus ); but T. dissimilis was the least sensitive invertebrate species to synthetic...midge) Lumbriculus variegatus (oligochaete) Fish Pimephales promelas (fathead minnow) Lepomis machrochirus (bluegill sunfish) Ictalurus punctatus...day bioconcentration test data were less than 100 in bluegills, D. magna, and L. variegatus , but 2100 to 2500 in the alga S. capricornutum. In bluegills

  11. [Structural disorders in the tissues of nematodes of the suborder Ascaridata after exposure to anthelmintics].

    PubMed

    Bogoiavlenskiĭ, Iu K; Rachkovskaia, N V; Chebyshev, N V

    1995-01-01

    The paper analyzes the authors' original findings and the data available in the literature concerning the structural changes in the tissue of Ascaridae after exposure to anthelmintics. The subject of the study of structural changes occurring in the digestive and genital systems represented As. canis lumbricoides, Toxocara mystax, Ascaridia galli. All the anthelmintics under study were found to greatly affect their hypodermis, the wall of the middle intestine and the epithelium of various parts of the genital tubes. All the agent differently caused vacuolization and impaired the integrity of cellular organoids. The middle intestine displayed a local or complete destruction of the brush border, as well as the swelling or pyknosis of epithelial nuclei. Each anthelminthic affected the same tissue of different helminths in a different manner. The nature and magnitude of structural derangements in the digestive and genital systems of Ascaridae depended on the properties of an agent and species-specific responses of nematodes. The used anthelmintics caused only insignificant structural changes in the excretory system.

  12. Endoparasites in mammals from seven zoological gardens in Romania.

    PubMed

    Dărăbuş, Gheorghe; Afrenie, Mihăită; Hotea, Ionela; Imre, Mirela; Morariu, Sorin

    2014-06-01

    Animals from seven zoological gardens located in Romania, including 18 species of herbivores, 10 species of carnivores, and 13 species of omnivores, were screened for the presence of parasites. Overall, the prevalences of parasites identified in the sampled population were 54.2% (58/107) for herbivores, 54.5% (24/44) for carnivores, and 32.6% (17/52) for omnivores. In herbivores, Eimeria spp., Dicrocelium lanceolatum, and pulmonary and digestive strongyles were detected. In carnivores, the genera Eimeria and Cystoisospora and nematodes from Ancylostomatidae, Strongyloidae, Ascaridae, Capillariidae and Trichocephalidae were identified. Of 13 omnivore species included in the study, parasites from Eimeridae, Ascaridae, Strongyloidae, and Trichocephalidae were identified in seven species. Toxoplasma antibodies were identified using enzyme-linked immunosorbent assay in all definitive hosts (lions and wild cats) examined. In intermediate hosts (herbivores and omnivores), antibodies to Toxoplasma gondii had a prevalence of 58.8%, except in wild boars (Sus scrofa), in which the prevalence was 100%.

  13. Origins of the parasitic habit in the nematoda.

    PubMed

    Clark, W C

    1994-12-01

    Circumstances that probably attended and influenced the adoption and development of the parasitic habit amongst the Nematoda are examined. Features that allowed early terrestrial nematodes to exploit discontinuous habitats such as decomposing organic matter, are considered to have been advantageous to microbivorous Secernentea that became parasites of animals and plants. This development followed the appearance of a land flora and that the Amphibia were the first vertebrate hosts of nematodes. Life cycles involving intermediate hosts were essential in drier environments and in a aquatic ones where intermediate hosts preserve the infective stages; keeps them "in circulation", and makes them attractive to predators. It is concluded that the parasitic habit was adopted repeatedly in both Secernentea and Adenophorea, though the latter did not diversify as much. Convergence is a common feature of nematode evolution, and the typical life history pattern of 5 stadia separated by 4 moults is often greatly modified by suppression, extension and diversification of stages and their roles. There is a need to examine the nematodes, especially of invertebrates in the remaining rain forests of Gondwanaland before they disappear.

  14. Subclinical effects and fenbendazole treatment of turkey ascaridiasis under simulated field conditions.

    PubMed

    Yazwinski, T A; Tucker, C; Stelzleni, A; Johnson, Z; Robins, J; Downum, K; Fincher, M; Matlock, J; Chapman, H D

    2002-01-01

    Under simulated natural conditions of bird production and parasite challenge, the effects of ascaridiasis and the effectiveness of fenbendazole treatment (6-day regimes in the feed at 16 ppm) were documented. Birds were artificially challenged with ascarid larvae on a daily basis from day 35 to 112, with bird grow out ending on day 119. Experimental groups, on a per pen basis, were infected control, treated with fenbendazole at days 63-69, treated with fenbendazole at days 63-69 and days 91-97, and uninfected control. In the same order as above, and on an experimental group mean bird basis, final weights were 13.34, 13.47, 13.59, and 13.78 kg, average daily gains from day 7 to day 119 were 117.8, 118.9, 120.1, and 121.8 g, and units gained per unit of feed consumed from day 7 to day 119 were 0.337, 0.341, 0.347, and 0.362. Infected control bird mean Ascaridia dissimilis burdens, with all stages combined, ranged from 351.1 on day 63 to 117.2 on day 91, levels seen commonly with naturally infected commercial turkeys. Trial data dearly indicated that moderate A. dissimilis burdens negatively impacted animal performance (average daily gains and feed efficiencies) and that these parasite burdens are effectively removed by fenbendazole treatment.

  15. Taxonomic notes on Holcobunus Roewer, 1910, with descriptions of three new species, and new records for Holcobunus nigripalpis Roewer, 1910 (Opiliones: Eupnoi: Sclerosomatidae).

    PubMed

    Tourinho, Ana Lúcia; Pinto-da-Rocha, Ricardo; Bragagnolo, Cibele

    2015-10-05

    Three new Brazilian species of Holcobunus Roewer, 1910 are described, thus increasing the total number of species in the genus to five: Holcobunus bicornutus Mello-Leitão, 1940, H. nigripalpis Roewer, 1910, Holcobunus dissimilis sp. nov. (type locality: Espírito Santo, Santa Teresa, Reserva Biologia Augusto Ruschi), Holcobunus ibitirama sp. nov. (type locality: Espírito Santo, Ibitirama, Santa Marta, close to Parque Nacional Caparaó), and Holcobunus uaisoh sp. nov. (type locality: Minas Gerais, Fervedouro, Parque Estadual Serra do Brigadeiro). A new record for Holcobunus nigripalpis Roewer, 1910 from Minas Gerais is also provided and the morphological variation in both penis and somatic morphology in the genus are presented and discussed. These observations enhance our understanding of both the diversity and distribution of Holcobunus.

  16. New data on the taxonomy and distribution of ten Neotropical chewing lice of the genus Myrsidea (Phthiraptera: Menoponidae), including the description of a new species.

    PubMed

    Kolencik, Stanislav; Sychra, Oldrich; Valan, Miroslav; Literak, Ivan

    2016-03-02

    The new species Myrsidea alexanderi is described and illustrated ex Pheugopedius maculipectus (Troglodytidae) from Honduras. Redescriptions and illustrations are given for both sexes of Myrsidea chiapensis ex Calocitta formosa from Costa Rica, and the male of M. dissimilis ex Progne chalybea from Brazil. Also, seven other previously known species or subspecies of the louse genus Myrsidea are recorded and discussed from passerine birds of the Neotropical Region, as follows: Myrsidea antiqua, Myrsidea balteri, Myrsidea diffusa, Myrsidea nesomimi borealis, Myrsidea paleno, Myrsidea psittaci and Myrsidea serini. Our data increase knowledge of intraspecific morphological variability within these species, and also of their host and geographical distribution. New host-louse associations are: Agelaioides badius for M. psittaci; Basileuterus culicivorus and Myiothlypis leucoblephara for M. paleno; Mimus saturninus for M. nesomimi borealis; and Icterus dominicensis and Molothrus rufoaxillaris for Myrsidea sp.

  17. Phylogenetic relationships of Amazonian anole lizards (Dactyloa): taxonomic implications, new insights about phenotypic evolution and the timing of diversification.

    PubMed

    Prates, Ivan; Rodrigues, Miguel Trefaut; Melo-Sampaio, Paulo Roberto; Carnaval, Ana Carolina

    2015-01-01

    The ecology and evolution of Caribbean anoles are well described, yet little is known about mainland anole species. Lack of phylogenetic information limits our knowledge about species boundaries, morphological evolution, and the biogeography of anoles in South America. To help fill this gap, we provide an updated molecular phylogeny of the Dactyloa (Dactyloidae), with emphasis on the punctata species group. By sampling understudied Amazonian taxa, we (i) assess the phylogenetic placement of the 'odd anole', D. dissimilis; (ii) infer the relationships of the proboscis-bearing D. phyllorhina, testing the hypothesis of independent nasal appendage evolution within the anole radiation; and (iii) examine genetic and dewlap color variation in D. punctata and D. philopunctata. Combining multiple nuclear loci with a review of the fossil record, we also (iv) estimate divergence times within the pleurodont iguanian clade of lizards, including Amazonian representatives of Dactyloa and Norops (Dactyloidae) and of Polychrus (Polychrotidae). We recover the five Dactyloa clades previously referred to as the aequatorialis, heteroderma, latifrons, punctata and roquet species groups, as well as a sixth clade composed of D. dissimilis and the non-Amazonian D. neblinina and D. calimae. We find D. phyllorhina to be nested within the punctata group, suggesting independent evolution of the anole proboscis. We consistently recover D. philopunctata nested within D. punctata, and report limited genetic divergence between distinct dewlap phenotypes. The most recent common ancestor of Dactyloa, Anolis and Norops dates back to the Eocene. Most Amazonian taxa within both Dactyloa and Norops diverged in the Miocene, but some diversification events were as old as the late Eocene and late Oligocene. Amazonian Polychrus diverged in the Pliocene. Our findings have broad implications for anole biogeography, disputing recent suggestions that modern dactyloid genera were present in the Caribbean region

  18. Automated identification of copepods using digital image processing and artificial neural network

    PubMed Central

    2015-01-01

    Background Copepods are planktonic organisms that play a major role in the marine food chain. Studying the community structure and abundance of copepods in relation to the environment is essential to evaluate their contribution to mangrove trophodynamics and coastal fisheries. The routine identification of copepods can be very technical, requiring taxonomic expertise, experience and much effort which can be very time-consuming. Hence, there is an urgent need to introduce novel methods and approaches to automate identification and classification of copepod specimens. This study aims to apply digital image processing and machine learning methods to build an automated identification and classification technique. Results We developed an automated technique to extract morphological features of copepods' specimen from captured images using digital image processing techniques. An Artificial Neural Network (ANN) was used to classify the copepod specimens from species Acartia spinicauda, Bestiolina similis, Oithona aruensis, Oithona dissimilis, Oithona simplex, Parvocalanus crassirostris, Tortanus barbatus and Tortanus forcipatus based on the extracted features. 60% of the dataset was used for a two-layer feed-forward network training and the remaining 40% was used as testing dataset for system evaluation. Our approach demonstrated an overall classification accuracy of 93.13% (100% for A. spinicauda, B. similis and O. aruensis, 95% for T. barbatus, 90% for O. dissimilis and P. crassirostris, 85% for O. similis and T. forcipatus). Conclusions The methods presented in this study enable fast classification of copepods to the species level. Future studies should include more classes in the model, improving the selection of features, and reducing the time to capture the copepod images. PMID:26678287

  19. Automated identification of copepods using digital image processing and artificial neural network.

    PubMed

    Leow, Lee Kien; Chew, Li-Lee; Chong, Ving Ching; Dhillon, Sarinder Kaur

    2015-01-01

    Copepods are planktonic organisms that play a major role in the marine food chain. Studying the community structure and abundance of copepods in relation to the environment is essential to evaluate their contribution to mangrove trophodynamics and coastal fisheries. The routine identification of copepods can be very technical, requiring taxonomic expertise, experience and much effort which can be very time-consuming. Hence, there is an urgent need to introduce novel methods and approaches to automate identification and classification of copepod specimens. This study aims to apply digital image processing and machine learning methods to build an automated identification and classification technique. We developed an automated technique to extract morphological features of copepods' specimen from captured images using digital image processing techniques. An Artificial Neural Network (ANN) was used to classify the copepod specimens from species Acartia spinicauda, Bestiolina similis, Oithona aruensis, Oithona dissimilis, Oithona simplex, Parvocalanus crassirostris, Tortanus barbatus and Tortanus forcipatus based on the extracted features. 60% of the dataset was used for a two-layer feed-forward network training and the remaining 40% was used as testing dataset for system evaluation. Our approach demonstrated an overall classification accuracy of 93.13% (100% for A. spinicauda, B. similis and O. aruensis, 95% for T. barbatus, 90% for O. dissimilis and P. crassirostris, 85% for O. similis and T. forcipatus). The methods presented in this study enable fast classification of copepods to the species level. Future studies should include more classes in the model, improving the selection of features, and reducing the time to capture the copepod images.

  20. Mitochondrial DNA Sequence Divergence among Meloidogyne incognita, Romanomermis culicivorax, Ascaris suum, and Caenorhabditis elegans

    PubMed Central

    Powers, T. O.; Harris, T. S.; Hyman, B. C.

    1993-01-01

    Mitochondrial DNA sequences were obtained from the NADH dehydrogenase subunit 3 (ND3), large rRNA, and cytochrome b genes from Meloidogyne incognita and Romanomermis culicivorax. Both species show considerable genetic distance within these same genes when compared with Caenorhabditis elegans or Ascaris suum, two species previously analyzed. Caenorhabditis, Ascaris, and Meloidogyne were selected as representatives of three subclasses in the nematode class Secernentea: Rhabditia, Spiruria, and Diplogasteria, respectively. Romanomermis served as a representative out-group of the class Adenophorea. The divergence between the phytoparasitic lineage (represented by Meloidogyne) and the three other species is so great that virtually every variable position in these genes appears to have accumulated multiple mutations, obscuring the phylogenetic information obtainable from these comparisons. The 39 and 42% amino acid similarity between the M. incognita and C. elegans ND3 and cytochrome b coding sequences, respectively, are approximately the same as those of C. elegans-mouse comparisons for the same genes (26 and 44%). This discovery calls into question the feasibility of employing cloned C. elegans probes as reagents to isolate phytoparasitic nematode genes. The genetic distance between the phytoparasitic nematode lineage and C. elegans markedly contrasts with the 79% amino acid similarity between C. elegans and A. suum for the same sequences. The molecular data suggest that Caenorhabditis and Ascaris belong to the same subclass. PMID:19279810

  1. Sequences homologous to Tc(s) transposable elements of Caenorhabditis elegans are widely distributed in the phylum nematoda.

    PubMed

    Abad, P; Quiles, C; Tares, S; Piotte, C; Castagnone-Sereno, P; Abadon, M; Dalmasso, A

    1991-09-01

    To have a better understanding of the evolutionary history of mobile elements within the nematodes, we examined the distribution and the conservation of homologues to transposable elements from Caenorhabditis elegans (Tc1, Tc2, Tc3, Tc4, Tc5, and FB1) in 19 nematode species belonging to the class Secernentea. Our results show that Tc1 elements display a distribution restricted to the family Rhabditidae with poor conservation. The Tc2 and FB1 homologous elements have the same patchy distribution within the Rhabditidae. They were only found in Caenorhabditis and in Teratorhabditis. The Tc3 element is widely distributed among nematode species. Tc3 homologous elements are present in the majority of the Rhabditidae but also in two genera within the family Panagrolaimidae, and in Bursaphelenchus, which belongs to the order Aphelenchida. Tc4 and Tc5 homologues show the most limited distribution of all tested elements, being strictly limited to C. elegans. These data indicate that in some cases, the distribution of transposable elements in the nematode cannot be explained by strict vertical transmission. The distribution of Tc3, Tc4, and Tc5 suggests that horizontal transmission may have occurred between reproductively isolated species during their evolutionary history.

  2. A quick tour of nematode diversity and the backbone of nematode phylogeny.

    PubMed

    De Ley, Paul

    2006-01-25

    Contrary to textbook dogma, nematodes are not only highly diverse, but often also complex and biologically specialized metazoans. Just a few of the many fascinating adaptations are reviewed in this chapter, as a prelude to a quick tour through phylogenetic relationships within the phylum. Small Subunit rDNA sequences have confirmed several controversial prior hypotheses, as well as revealing some unexpected relationships, resulting in a recent proposal for revised classification. Three major lineages exist within the phylum: Chromadoria, Enoplia and Dorylaimia. The exact order of appearance of these lineages is not yet resolved, which also leaves room for uncertainty about the biology and morphology of the exclusive common ancestor of nematodes. Enoplia and Dorylaimia differ considerably in many respects from C. elegans, which is a member of Chromadoria. The latter group is extremely diverse in its own right, for example in ecological range, in properties of the cuticle and in structure of the pharynx. The formerly relatively widely accepted class Secernentea is deeply nested within Chromadoria, and has therefore recently been relegated to the rank and name of order Rhabditida. Within this order, closer relatives of C. elegans include strongylids, diplogasterids and bunonematids. Tylenchs, cephalobs and panagrolaimids are also members of Rhabditida, albeit probably more distantly related to C. elegans.

  3. Biodiversity and distribution of helminths and protozoa in naturally infected horses from the biosphere reserve La Sierra Madre de Chiapas", México.

    PubMed

    Güiris, A D M; Rojas, H N M; Berovides, A V; Sosa, P J; Pérez, E M E; Cruz, A E; Chávez, H C; Moguel, A J A; Jimenez-Coello, M; Ortega-Pacheco, A

    2010-06-24

    A cross sectional survey was performed to identify gastrointestinal helminths and protozoans in naturally infected horses from the biosphere reserve known as "La Sierra Madre de Chiapas", Mexico (El Triunfo and La Sepultura). During a three-year survey, fecal samples from 90 horses and parasites from 2 necropsied animals were collected. Five families from the Nematoda class: Ascaridae, Kathlanidae, Oxyuridae, Strongylidae and Trichostrongylidae were found, whereas, only one family from the class Cestoda, was observed: Anoplocephalidae. One family from the class Insecta, was observed: Gasterophiilidae. The number of species of parasites ranged from 13 to 18 with an average of 15 per animal. Adult parasites were recovered from the large intestine luminal contents at necropsy. Species recovered included: Strongylus vulgaris, S. equinus, S. edentatus, Oxyuris equi, Parascaris equorum, Coronocyclus coronatum, C. labiatus, C. labratus, Cyathostomum tetracanthum, Cylicocyclus insigne, C. leptostomus, Cylicodontophorus bicoronatus, Cylicostephanus asymetricus, C. bidentatus, C. minutus, C. longibursatus, Petrovinema poculatum, Poteriostomum imparidentatum, Cylicostephanus goldi, Tridentoinfundibulum gobi, Triodontophorus serratus and T. tenuicollis. One species of Diptera were recovered from stomach and identified: Gasterophilus intestinalis. Furthermore, different species of protozoa were recovered from fresh horse-dung and identified in four classes: Sporozoa, Litostomatea, Ciliasida and Suctoria. Nine families: Cryptosporidiidae, Eimeriidae, Balantidiidae, Buetschliidae, Blepharocorythidae, Cycloposthiidae, Spirodiniididae, Ditoxidae, Acinetidae; and 31 ciliates species were recorded: Allantosoma dicorniger, A. intestinalis, Alloiozona trizona, Blepharosphaera intestinalis, Blepharoprosthium pireum, Blepharoconus benbrooki, Bundleia postciliata, Didesmis ovalis, D. quadrata, Sulcoarcus pellucidulus, Blepharocorys angusta, B. cardionucleata, B. curvigula, B. juvata, B

  4. Anti-angiogenic activities of CRBGP from buccal glands of lampreys (Lampetra japonica).

    PubMed

    Jiang, Qi; Liu, Yu; Duan, Dandan; Gou, Meng; Wang, Hao; Wang, Jihong; Li, Qingwei; Xiao, Rong

    2016-04-01

    Cysteine-rich secretory proteins (CRISPs), characterized by 16 conserved cysteines, are distributed in a wide range of organisms, such as secernenteas, amphibians, reptiles and mammals. In the previous studies, a novel CRISP family member (cysteine-rich buccal gland protein, CRBGP) was separated from the buccal gland of lampreys (Lampetra japonica, L. japonica). Lamprey CRBGP could not only suppress depolarization-induced contraction of rat tail arterial smooth muscle, but also block voltage-gated sodium channels (VGSCs). In the present study, the anti-angiogenic activities of lamprey CRBGP were investigated using endothelial cells and chick chorioallantoic membrane (CAM) models. In vitro assays, lamprey CRBGP is able to induce human umbilical vein endothelial cells (HUVECs) apoptosis by disturbing the calcium homeostasis and mitochondria functions. In addition, lamprey CRBGP could inhibit proliferation, adhesion, migration, invasion and tube formation of HUVECs by affecting the organization of F-actin and expression level of matrix metallo-proteinase 2 (MMP-2), matrix metallo-proteinase 9 (MMP-9) and vascular endothelial growth factor A (VEGFA) which are related to angiogenesis. In vivo assays, lamprey CRBGP could suppress the blood vessel formation in CAM models. Therefore, lamprey CRBGP is an important protein present in the buccal gland of lampreys and might help lampreys suppress the contraction of blood vessels, nociceptive responses and wound healing of host fishes during their feeding time. In addition, lamprey CRBGP might have the potential to act as an effective anti-angiogenic factor for the treatment of abnormal angiogenesis induced diseases.

  5. An improved molecular phylogeny of the Nematoda with special emphasis on marine taxa.

    PubMed

    Meldal, Birgit H M; Debenham, Nicola J; De Ley, Paul; De Ley, Irma Tandingan; Vanfleteren, Jacques R; Vierstraete, Andy R; Bert, Wim; Borgonie, Gaetan; Moens, Tom; Tyler, Paul A; Austen, Melanie C; Blaxter, Mark L; Rogers, Alex D; Lambshead, P J D

    2007-03-01

    Phylogenetic reconstructions of relations within the phylum Nematoda are inherently difficult but have been advanced with the introduction of large-scale molecular-based techniques. However, the most recent revisions were heavily biased towards terrestrial and parasitic species and greater representation of clades containing marine species (e.g. Araeolaimida, Chromadorida, Desmodorida, Desmoscolecida, Enoplida, and Monhysterida) is needed for accurate coverage of known taxonomic diversity. We now add small subunit ribosomal DNA (SSU rDNA) sequences for 100 previously un-sequenced species of nematodes, including 46 marine taxa. SSU rDNA sequences for >200 taxa have been analysed based on Bayesian inference and LogDet-transformed distances. The resulting phylogenies provide support for (i) the re-classification of the Secernentea as the order Rhabditida that derived from a common ancestor of chromadorean orders Araeolaimida, Chromadorida, Desmodorida, Desmoscolecida, and Monhysterida and (ii) the position of Bunonema close to the Diplogasteroidea in the Rhabditina. Other, previously controversial relationships can now be resolved more clearly: (a) Alaimus, Campydora, and Trischistoma belong in the Enoplida, (b) Isolaimium is placed basally to a big clade containing the Axonolaimidae, Plectidae, and Rhabditida, (c) Xyzzors belongs in the Desmodoridae, (d) Comesomatidae and Cyartonema belongs in the Monhysterida, (e) Globodera belongs in the Hoplolaimidae and (f) Paratylenchus dianeae belongs in the Criconematoidea. However, the SSU gene did not provide significant support for the class Chromadoria or clear evidence for the relationship between the three classes, Enoplia, Dorylaimia, and Chromadoria. Furthermore, across the whole phylum, the phylogenetically informative characters of the SSU gene are not informative in a parsimony analysis, highlighting the short-comings of the parsimony method for large-scale phylogenetic modelling.

  6. Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown Clades.

    PubMed

    Holterman, Martijn; van der Wurff, Andre; van den Elsen, Sven; van Megen, Hanny; Bongers, Tom; Holovachov, Oleksandr; Bakker, Jaap; Helder, Johannes

    2006-09-01

    Inference of evolutionary relationships between nematodes is severely hampered by their conserved morphology, the high frequency of homoplasy, and the scarcity of phylum-wide molecular data. To study the origin of nematode radiation and to unravel the phylogenetic relationships between distantly related species, 339 nearly full-length small-subunit rDNA sequences were analyzed from a diverse range of nematodes. Bayesian inference revealed a backbone comprising 12 consecutive dichotomies that subdivided the phylum Nematoda into 12 clades. The most basal clade is dominated by the subclass Enoplia, and members of the order Triplonchida occupy positions most close to the common ancestor of the nematodes. Crown Clades 8-12, a group formerly indicated as "Secernentea" that includes Caenorhabditis elegans and virtually all major plant and animal parasites, show significantly higher nucleotide substitution rates than the more basal Clades 1-7. Accelerated substitution rates are associated with parasitic lifestyles (Clades 8 and 12) or short generation times (Clades 9-11). The relatively high substitution rates in the distal clades resulted in numerous autapomorphies that allow in most cases DNA barcode-based species identification. Teratocephalus, a genus comprising terrestrial bacterivores, was shown to be most close to the starting point of Secernentean radiation. Notably, fungal feeding nematodes were exclusively found basal to or as sister taxon next to the 3 groups of plant parasitic nematodes, namely, Trichodoridae, Longidoridae, and Tylenchomorpha. The exclusive common presence of fungivorous and plant parasitic nematodes supports a long-standing hypothesis that states that plant parasitic nematodes arose from fungivorous ancestors.

  7. [Species, dynamics and population composition of phthirapteran in free-range chickens (Gallus gallus L.) in São Luis Island, State of Maranhão].

    PubMed

    Guerra, Rita de M S N de C; Chaves, Elba P; Passos, Tarsila M G; Santos, Ana C G Dos

    2008-01-01

    The objective of this work was to identify the phthirapteran species, to determine the prevalence according to the anatomical region of the body and to know the dynamics and composition of the population of these ectoparasites in free-range chicken in São Luis Island, state of Maranhão. Inspection was performed in 40 chickens and feathers were collected from the head, neck, wing, thigh, dorsal and ventral regions and cloacae. The phthirapteran species identified were: Menopon gallinae L., Menacanthus stramineus Nitzsch, Menacanthus pallidulus Neumann, Menacanthus cornutus Schommer (Menoponidae), and Lipeurus caponis L., Goniodes dissimilis Denny and Goniocotes gallinae De Geer (Philopteridae). L. caponis was collected from all regions sampled, including the head, which was the least infested region. The dorsum was the most infested, especially in the dry period of the year and where the greater parasitic diversity was observed, the wing and the head were the least infested regions. Considering the dynamics and the composition of the population the phthirapteran presented a prevalence of 85% of the sampled chickens, the mean intensity of infestation was 45.3 varying from <1 to 453. The egg stage was superior to the others life stages followed by nymphs and female adults, independent of the phthirapteran species.

  8. Interruption of the semiochemical-based attraction of ambrosia beetles to ethanol-baited traps and ethanol-injected trap trees by verbenone.

    PubMed

    Ranger, Christopher M; Tobin, Patrick C; Reding, Michael E; Bray, Alicia M; Oliver, Jason B; Schultz, Peter B; Frank, Steven D; Persad, Anand B

    2013-06-01

    We examined the extent to which verbenone, a bark beetle antiaggregation pheromone, interrupted the semiochemical-based attraction of ambrosia beetles. Field trapping studies conducted in Ohio showed that a verbenone dispenser with a release rate of 50 mg/d at 25°C reduced the attraction of Anisandrus sayi Hopkins, Euwallacea validus (Eichhoff), Hypothenemus dissimilis (Zimmermann), Xylosandrus germanus (Blandford), and Xyleborinus saxesenii (Ratzeburg) to ethanol-baited traps. A verbenone dispenser attached to ethanol-injected Magnolia virginiana L. trap trees deployed in Ohio also reduced ambrosia beetle attacks compared to trap trees without a verbenone dispenser. Subsequent field trials demonstrated a direct relationship between distance from a verbenone dispenser and ambrosia beetle attacks on trap trees in Ohio in 2011 and 2012 and Tennessee in 2012, but not in Tennessee and Virginia in 2011. Assessment of the influence of verbenone on the probability of attacks above a density threshold found that although attacks occurred on trap trees regardless of their proximity to a verbenone dispenser, the higher density of attacks per tree occurred on trap trees farthest away from the verbenone source in Ohio and Tennessee. Verbenone alone could be somewhat useful for discouraging ambrosia beetle attacks on individual trees or on a small spatial scale, but deployment of verbenone might be most effective when integrated as part of a "push-pull" strategy.

  9. Genetic and biogeographical relationships among species of Astyanax (Teleostei, Characidae) in Brazilian river basins.

    PubMed

    Mello, R; Maniglia, T C; Prioli, S M A P; Prioli, A J

    2015-11-30

    The genetic relationships among 17 species of Astyanax from the Iguaçu River and adjacent river basins in Brazil were examined using nucleotide sequences of the mitochondrial cytochrome b gene (cytb). Congruent trees were constructed using neighbor-joining, maximum-likelihood, and Bayesian methods. The resulting clades suggest that at least three major groups share similar origins with the endemic species of the Iguaçu River. The results indicate that Astyanax is polyphyletic in this location, which suggests that Astyanax did not diversify from a single ancestral group that was isolated when the Iguaçu River basin formed. Astyanax bifasciatus shares an origin with some species of the altiparanae-bimaculatus complex, while A. minor originated from the same group as A. aff. paranae, A. fasciatus, and A. bockmanni. The third group includes A. dissimilis, Astyanax sp F, and A. serratus that are endemic species to the Iguaçu River basin. Geological and hydrological events that influenced the biogeographical patterns of these species are discussed.

  10. [Composition and characteristics of understory bird communities in monsoon evergreen broadleaved forest of Mengyang, Xishuangbanna in the dry season].

    PubMed

    Huang, Jun-Hui; Zou, Fa-Sheng

    2010-06-01

    Understory birds in monsoon evergreen broadleaved forest of Mengyang, Xishuangbanna, Yunnan were sampled using mist nets from October 2008 to March 2009. A total of 1423 individuals of 90 species belonging to 28 families and 7 orders were captured, among which there are 8 dominant bird species, i.e., Sliver-breasted Broadbill (Serilophus lunatus), Brown-cheeked Fulvatta (Alcippe poioicephala), White-throated Bulbul (Alophoixus pallidus), Golden-spectacled Warbler (Seicercus burkii), White-tailed Robin (Cinclidium leucurum), Black-breasted Thrush (Turdus dissimilis), Streak-Breasted Jungle Babbler (Pellorneum ruficeps), and Buff-breasted Jungle Babbler (Trichastoma tickelli). Resident birds, the major composition of the understory birds, accounted for 89.3 percent of total captures. The mean capture rate was 9.0+-3.7 individuals/(100 net-hours) and it differed significantly between months, highest in December 2008 (12.5+-1.3)individuals/(100 net-hours), lowest in February 2009 (5.2+-0.6) individuals/(100 net-hours). The most significant characteristic of understory birds in Xishuangbanna is more species abundance and lower species density compared to understory bird composition of South China. Another characteristic is that no obvious dominance pattern was observed in Babbler species. We also noticed that silver-breasted Broadbill, National Key Protection Bird, is the most abundance species in our captures, thus their habitat require further protection.

  11. Hard ticks (Acari: Ixodidae) of livestock in Nicaragua, with notes about distribution.

    PubMed

    Düttmann, Christiane; Flores, Byron; Kadoch Z, Nathaniel; Bermúdez C, Sergio

    2016-09-01

    We document the species of ticks that parasitize livestock in Nicaragua. The study was based on tick collection on cattle and horses from 437 farms in nine departments. Of 4841 animals examined (4481 cows and 360 horses), 3299 were parasitized, which represent 68 % of the bovines and 67 % of the equines in study: 59 cows and 25 horses were parasitized by more than one species. In addition, 280 specimens of the entomological museum in León were examined. The ticks found on cattle were Rhipicephalus microplus (75.2 % of the ticks collected), Amblyomma mixtum (20.8 %), A. parvum (2.6 %), A. tenellum (0.7 %), A. maculatum (0.7 %). While the ticks collected from the horses were: Dermacentor nitens (41.5 %), A. mixtum (31.7 %), R. microplus (13.8 %), A. parvum (6.5 %), A. tenellum (3.3 %), D. dissimilis (2.4 %) and A. maculatum (0.8 %).

  12. Early to middle miocene sequence stratigraphy of well-5 (OML 34), Niger Delta, Nigeria

    NASA Astrophysics Data System (ADS)

    Nwaejije, E. C.; Hamidu, I.; Obiosio, E. O.

    2017-05-01

    A sequence stratigraphic study was carried out on ditch cutting samples from well-5 (OML 34) in the Niger Delta in Nigeria. The sequence stratigraphic analysis based on foraminifera content and lithologic descriptions has revealed the following: five systems tracts (two transgressive systems tracts, two highstand systems tracts and one lowstand system tract); two third order maximum flooding surfaces (proposed at 3388 m and 3557 m dated 15.9 Ma and 17.4 Ma respectively) and two sequence boundaries proposed at 3301 m and 3466 m dated 15.5 Ma and 16.7 Ma respectively with the aid of index foraminifers present in the well (Praeorbulina glomerosa, Praeorbulina sicana, Orbulina saturalis and Catapsydrax dissimilis), as a result, three depositional sequences were established from the well intervals. These depositional sequences with the foraminifera species present in the well revealed that the well interval was exposed to two local depositional cycles (cycle 6 and 7), two regional cycles (cycles 2.2 and 2.3) within the TB2 super cycles corresponding to Haq et al. (1988) chronostratigraphic chart.

  13. Chronic toxicity of water-soluble fractions of fresh and water-leached solvent refined coal-II liquids to a freshwater benthic invertebrate

    SciTech Connect

    Dauble, D.D.; Fallon, W.E.; Gray, R.H.; Bean, R.M.

    1981-01-01

    A solvent refined coal (SRC-II) liquid blend (2.9:1, middle to heavy distillate) obtained from a pilot plant was sequentially extracted with water to observe compositional changes in water soluble fractions (WSF). An initial WSF, designed to simulate chemicals present after contact with water, and a water-leached (artificially weathered) WSF, designed to represent conditions after exposure to the aqueous environment were chemically characterized. Toxicities of the two WSFs were compared by observing chronic effects on a freshwater benthic invertebrate, Tanytarsus dissimilis. Survival to emergence was suppressed at 3.0 ppth of the initial WSF and at 9.4 ppth of the artificially weathered WSF. However, based on equal concentrations of organic carbon and phenolics, weathered WSF solutions were three to five times more toxic. Concentrations, relative distributions, and loss over time of phenols and aromatic hydrocarbons differed between aqueous extractions derived from the fresh and weathered SRC II material. Tests assessing long-term effects of complex materials must be designed and interpreted on the basis of organism exposure to compounds most likely to persist in the environment.

  14. Cumberlandian Mollusk Conservation Program. Activity 3: identification of fish hosts. [Conradilla caelata; Quadrula intermedia; Epioblasma brevidens; Epioblasma capsaeformis; Epioblasma triquetra; Quadrula cylindrica; Carunculina moesta

    SciTech Connect

    Hill, D.M.

    1986-02-01

    A key element of the Cumberlandian Mollusk Conservation Program undertaken by TVA in 1979 was the determination of fish hosts of Cumberlandian mussel species unique to the Tennessee River drainage and especially the species whose habitat would be inundated by completion of Columbia Dam on the Duck River, Tennessee. Principal emphasis was placed on the birdwing pearly mussel, Conradilla caelata and the Cumberland monkeyface, Quadrula intermedia - two federally listed endangered species with limited distributions outside the proposed inundation zone of the Duck River. Additional species studied included three species of the genus Epioblasma (E. brevidens, E. capsaeformis, and E. triquetra), Quadrula cylindrica, Villosa iris, and Carunculina moesta. Experimental glochidial infection of 55 fish species resulted in the establishment of the following mussel-fish host relationships: Conradilla caelata - Etheostoma zonale; Quadrule intermedia - Hybopsis dissimilis, Hybopsis insignis; Epioblasma brevidens - Etheostoma blennioides, Etheostoma maculatum, Etheostoma rufilineatum, Etheostoma simoterum, Percina caprodes, Cottus carolinae; Epioblasma capsaeformis - Etheostoma maculatum, Etheostoma rufilineatum, Percina sciera, Cottus carolinae; Epioblasma triquetra - Percina caprodes, Cottus carolinae; Quadrula cylindrica - Notropis galacturus, Notropis spilopterus, Hybopsis amblops; and Carunculina moesta - Lepomis cyanellus, Lepomis megalotis.

  15. DNA barcoding as an aid for species identification in austral black flies (Insecta: Diptera: Simuliidae).

    PubMed

    Hernández-Triana, Luis M; Montes De Oca, Fernanda; Prosser, Sean W J; Hebert, Paul D N; Gregory, T Ryan; McMurtrie, Shelley

    2017-04-01

    In this paper, the utility of a partial sequence of the COI gene, the DNA barcoding region, for the identification of species of black flies in the austral region was assessed. Twenty-eight morphospecies were analyzed: eight of the genus Austrosimulium (four species in the subgenus Austrosimulium s. str., three species in the subgenus Novaustrosimulium, and one species unassigned to subgenus), two of the genus Cnesia, eight of Gigantodax, three of Paracnephia, one of Paraustrosimulium, and six of Simulium (subgenera Morops, Nevermannia, and Pternaspatha). The neighbour-joining tree derived from the DNA barcode sequences grouped most specimens according to species or species groups recognized by morphotaxonomic studies. Intraspecific sequence divergences within morphologically distinct species ranged from 0% to 1.8%, while higher divergences (2%-4.2%) in certain species suggested the presence of cryptic diversity. The existence of well-defined groups within S. simile revealed the likely inclusion of cryptic diversity. DNA barcodes also showed that specimens identified as C. dissimilis, C. nr. pussilla, and C. ornata might be conspecific, suggesting possible synonymy. DNA barcoding combined with a sound morphotaxonomic framework would provide an effective approach for the identification of black flies in the region.

  16. Parasitological and serological diagnosis of Strongyloides stercoralis in domesticated dogs from southeastern Brazil.

    PubMed

    Júnior, A Ferreira; Gonçalves-Pires, M R F; Silva, D A O; Gonçalves, A L R; Costa-Cruz, J M

    2006-03-15

    Canine strongyloidiasis is a parasitic infection caused by the nematode Strongyloides stercoralis and presents a great zoonotic potential. Its confirmation, using coproparasitological methods, is difficult. The detection of serum specific antibodies, however, may facilitate the diagnosis. The aims of this study were to determine the presence of S. stercoralis through the use of parasitological methods and to detect specific antibodies to the parasite in serum samples from domestic dogs by using the indirect fluorescent antibody test (IFAT) on slides and the enzyme-linked immunosorbent assay (ELISA). A total of 215 dogs of various breeds, from the cities of Uberlândia, Araxá and Campo Belo in the State of Minas Gerais, were examined and distributed according to age into the following groups: (I) 19 males and 20 females of 1-2 months old; (II) 11 males and 20 females of 2-month- to 1-year-old and (III) 41 males and 104 females, from 1 to 7 years old. Coproparasitological results showed that 63/215 (29.3%) of the dogs presented some kind of parasite, with two (0.9%) dogs (one from Araxá and the other from Uberlândia) passing S. stercoralis larvae in the feces. Serological results revealed antibodies to S. stercoralis in 45/215 (20.9%) of the dogs, with seropositivity rates of 0% (0/39) in Group I, 22.6% (7/31) in Group II, and 26.2% (38/145) in Group III. No serological cross-reactivity between S. stercoralis and hookworms or Ascaridae was found. Hookworm infections were seen in 31 dogs, but only one of these dogs (infected with both hookworm and Cystoisospora spp.) was S. stercoralis seropositive by IFAT. The present study demonstrated, for the first time, natural S. stercoralis infections in dogs diagnosed by coproparasitological and serological methods. It was concluded that the detection of specific antibodies to S. stercoralis by IFAT and ELISA may contribute to the diagnosis of canine strongyloidiasis.

  17. Dactylogyridean monogeneans of the siluriform fishes of the Old World.

    PubMed

    Lim, L H; Timofeeva, T A; Gibson, D I

    2001-11-01

    This is a catalogue and discussion of the known dactylogyridean monogenean genera of siluriform fishes of the Old World. Of a total of 38 nominal genera, only 19 are considered valid. Seventeen of these 19 genera are currently in the Ancyrocephalidae (containing the Ancyrocephalinae and Ancylodiscoidinae), whilst the other two (Neocalceostoma and Neocalceostomoides) are in the Neocalceostomatidae. The 17 genera are Anchylodiscus, Ancylodiscoides, Bagrobdella, Bifurcohaptor, Bychowskyella, Chauhanellus, Cornudiscoides, Hamatopeduncularia, Mizelleus, Paraquadriacanthus, Pseudancylodiscoides, Protoancylodiscoides, Quadriacanthus, Schilbetrema, Schilbetrematoides, Synodontella and Thaparocleidus. Clariotrema Long, 1981 and Neobychowskyella Ma, Wang & Li, 1983 are considered synonyms of Bychowskyella Akhmerov, 1952, Anacornuatus Dubey, Gupta & Agarwal, 1992 is considered a synonym of Quadriacanthus Paperna, 1961, Mizellebychowskia Gupta & Sachdeva, 1990 is considered a synonym of Neocalceostoma Tripathi, 1959 and Hargitrema Tripathi, 1959 is treated as a synonym of Hamatopeduncularia Yamaguti, 1953. It is proposed that the Ancylodiscoidinae be raised to family status within the order Dactylogyridea to accommodate these 17 'ancyrocephalid' genera from siluriforms, together with Malayanodiscoides and Notopterodiscoides from notopterids. A key and the diagnostic characteristics of the 19 recognised dactylogyridean genera from catfishes plus two from notopterids, together with a list of species and synonyms, are included. New combinations made in this work are Thaparocleidus avicularia (Chen, 1987) n. comb., T. calyciflorus (Chen, 1987) n. comb., T. choanovagina (Luo & Lang, 1981) n. comb., T. dissimilis (Chen, 1988) n. comb., T. leiocassis (Reichenbach-Klinke, 1959) n. comb., T. meticulosa (Chen, 1987) n. comb., T. parasoti (Zhao & Ma, 1999) n. comb., T. persculpus (Chen, 1987) n. comb., T. valga (Chen, 1987) n. comb. and T. wulingensis (Yao & Wang, 1997) n. comb. [all

  18. New species of the genus Chydaeus from China, Nepal, Myanmar, and Thailand, with remarks on species previously described (Coleoptera: Carabidae: Harpalini).

    PubMed

    Kataev, Boris M; Wrase, David W; Schmidt, Joachim

    2014-02-14

    Ten new species of the genus Chydaeus Chaudoir, 1854 are described: C. dissimilis sp. n. (the bedeli species group) from the Doi Inthanon Mountain, Chiang Mai Province, Thailand; C. shaanxiensis sp. n. (the kasaharai species group) from the Daba Shan Mountain Range, southern Shaanxi, China; C. kabaki sp. n. (the kasaharai species group) from southern Sichuan, China; C. weishanensis sp. n. (the kasaharai species group) from the northern part of Wuliang Shan, western Yunnan, China; C. belousovi sp. n. (the kasaharai species group) from the south-western Yunnan, China; C. putaoensis sp. n. (the kasaharai species group) from the environments of Putao, the northernmost part of Myanmar; C. ganeshensis sp. n. (the irvinei species group) from the Ganesh Himal, Central Nepal; C. chuliensis sp. n. (the semenowi species group) from the Manaslu Himal, Central Nepal; C. wuliangensis sp. n. (the semenowi species group) from the northern part of Wuliang Shan, western Yunnan, China; C. luxiensis sp. n. (the semenowi species group) from the environments of Luxi, western Yunnan, China. New data about the distribution of the following species are provided: C. kasaharai Ito, 2002, C. shunichii Ito, 2006, C. semenowi (Tschitschérine, 1899), C. convexus Ito, 2002, C. baoshanensis Kataev & Liang, 2012, and C. obtusicollis Schauberger, 1932. The following taxa are reported for the first time: Chydaeus semenowi, C. obtusicollis and C. bedeli interjectus Kataev & Schmidt, 2002 from the Indian state Arunachal Pradesh, C. bedeli difficilis Kataev & Schmidt, 2002 from Myanmar, and C. similis Kataev & Schmidt, 2002 from the Chinese provinces Shaanxi and Chongqing.

  19. Optimizing ethanol-baited traps for monitoring damaging ambrosia beetles (Coleoptera: Curculionidae, Scolytinae) in ornamental nurseries.

    PubMed

    Reding, Michael E; Schultz, Peter B; Ranger, Christopher M; Oliver, Jason B

    2011-12-01

    The exotic ambrosia beetles Xylosandrus crassiusculus (Motschulsky) and Xylosandrus germanus (Blandford) (Coleoptera: Curculionidae: Scolytinae) are serious pests in ornamental tree nurseries. To optimize bottle-traps as a monitoring system for X. crassiusculus and X. germanus in nurseries, we tested whether increasing the rate of commercial ethanol lures improved captures or early detection of these species. Experiments were conducted in Ohio (2008 and 2009) and Virginia (2008), two states that have experienced significant damage from X. crassiusculus, X. germanus, or both. There were four treatments: no-lure (unbaited control), 1-ethanol lure, 2-ethanol lures and 1 + 1-ethanol lures (one lure in the trap and one suspended 0.5 m above the trap). Captures of X. crassiusculus and X. germanus were higher in all ethanol treatments than unbaited controls, and were generally higher in treatments with two lures versus one. There was no difference in beetle captures between the 2-lure and 1 + 1-lure treatments. First detection of X. crassiusculus and X. germanus occurred more consistently in the treatments with two lures than one lure. Xyleborinus saxesenii (Ratzeburg), Anisandrus sayi Hopkins, Hypothenemus dissimilis Zimmermann, and Hypothenemus eruditus Westwood were also more attracted to traps baited with ethanol than unbaited controls. X. saxesenii was captured in higher numbers in the treatments with two lures than one in Virginia but not in Ohio. There was no difference in captures of the other species among ethanol treatments. The current research shows that ethanol release rates influence sensitivity of traps for detecting emergence of overwintered ambrosia beetles.

  20. Integrated biostratigraphy of the upper Oligocene-middle Miocene successions in west central Sinai, Egypt

    NASA Astrophysics Data System (ADS)

    Hewaidy, Abdel Galil A.; Farouk, Sherif; Ayyad, Haitham M.

    2014-12-01

    The nannofossil and planktonic foraminiferal biostratigraphy in four upper Oligocene-middle Miocene sections are examined in Nukhul-Sudr area of west central Sinai, Egypt. The integration of calcareous nannofossils and planktonic foraminifera is used to verify the ages and determine the biozones of the upper Oligocene-middle Miocene units in the studied area. This target is important in the light of the great lithofacies changes during the time interval. The detailed examination of the nannofossil and planktonic foraminiferal contents in these sections led to identification of 86 calcareous nannofossil species belonging to 22 genera, 10 families and 3 orders, in addition to 64 planktonic foraminiferal species belonging to 11 genera, 4 families and 2 superfamilies. The identified nannofossil and planktonic foraminiferal assemblages allow to distinguish five calcareous nannofossil biozones and six planktonic foraminiferal biozones. The biostratigraphic integration suggested the Chattian-Aquitanian age for the Nukhul Formation where the Globigerina ciperoensis Zone (P22) and Globigerinoides primordius Zone (M1a) correspond to calcareous nannofossil Sphenolithus ciperoensis Zone (NP25) and Discoaster druggii Zone (NN2), respectively. The Rudeis Formation is assigned to the Burdigalian-Langhian age depending on correspondence of Catpsydrax dissimilis Zone (M2), Globigerinoides bisphericus Zone (M4b) and Praeorbulina sicana Zone (M5) with Discoaster druggii zone (NN2), Sphenolithus belemnos Zone (NN3) and Helicosphaera ampliaperta Zone (NN4). The Somar Formation is found barren ofany microfossils, but it contains index pectens and oysters of Burdigalian age which may be equivalent to the lower part of the Rudeis Formation. The Kareem and Sarbut El-Gamal formations are represented by evaporitic and conglomeratic succession, where no foraminifera or nannofossils are recorded and assigned to the Langhian age according to their stratigraphic position. The Belayim Formation

  1. National progress in dengue vector control in Vietnam: survey for Mesocyclops (Copepoda), Micronecta (Corixidae), and fish as biological control agents.

    PubMed

    Nam, V S; Yen, N T; Holynska, M; Reid, J W; Kay, B H

    2000-01-01

    This paper describes the process of expanding a successful dengue control program in 3 provinces in northern Vietnam into a national one and demonstrates the presence of a rich, low-cost resource that could have similar applicability to other countries in the region. The cornerstone of the preventive strategy is larval control of Aedes aegypti (L.), the major vector, using predators such as copepods, Mesocyclops spp., aided by the corixid bug Micronecta quadristrigata Bredd, and fish in large water storage containers. From 1989 to 1998, 9 species of Mesocyclops (M. woutersi Van de Velde, M. aspericornis (Daday), M. ruttneri Kiefer, M. thermocyclopoides Harada, M. affinis Van de Velde, M. ogunnus Onabamiro, M. yenae Holynska, M. cf. pehpeiensis Hu, and M. dissimilis Defaye and Kawabata) were found in natural and artificial habitats in 26 provinces throughout Vietnam. The predatory capacities of 6 of these were evaluated in the laboratory. This indicated that daily consumption/killing averaged between 16 and 41 Ae. aegypti larvae per copepod. From detailed evaluations in 9 provinces, Mesocyclops spp. were surprisingly common in 8,413 artificial containers (concrete tanks, wells, ornamental ponds and in the south, large jars). Because of existing practices for washing and water transfer from ponds and lakes in Ha Tay and Ha Bac, Mesocyclops spp. already occurred in 60-100% of the water storage containers. When the relationship between the presence or absence of Mesocyclops and Aedes larvae in 5,111 containers was analyzed by the chi-square test, their distributions were significantly related, indicating control (odds ratio = 0.56). When 3,426 containers that did not contain Mesocyclops or fish were analyzed in relation to the distribution of Aedes larvae, those with Micronecta also had significantly less Aedes (odds ratio = 0.43). Therefore, this study demonstrates that there is an abundance of local Mesocyclops spp. in Vietnam that can be incorporated into

  2. Parasite diversity of European Myotis species with special emphasis on Myotis myotis (Microchiroptera, Vespertilionidae) from a typical nursery roost.

    PubMed

    Frank, Raphael; Kuhn, Thomas; Werblow, Antje; Liston, Andrew; Kochmann, Judith; Klimpel, Sven

    2015-02-15

    Bats belong to one of the most species-rich orders within the Mammalia. They show a worldwide distribution, a high degree of ecological diversification as well as a high diversity of associated parasites and pathogens. Despite their prominent and unique role, the knowledge of their parasite-host-relationships as well as the mechanisms of co-evolutionary processes are, partly due to strict conservation regulations, scarce. Juvenile specimens of the greater mouse-eared bat (Myotis myotis) from a roosting colony in Gladenbach (Hesse, Germany) were examined for their metazoan endo-and ectoparasite infections and pathogens. Morphometric data were recorded and the individuals were checked for Lyssavirus-specific antigen using a direct immunofluorescence test. For unambiguous species identification, the bats were analysed by cyt-b sequence comparison. Myotis myotis were parasitized by the six insect and arachnid ectoparasite species, i.e. Ixodes ricinus, Ischnopsyllus octactenus, Ichoronyssus scutatus, Steatonyssus periblepharus, Spinturnix myoti and Cimex dissimilis. Additionally, the nematode Molinostrongylus alatus and the cestode Vampirolepis balsaci were recorded. Each bat was parasitized by at least four species. The parasites showed partially extreme rates of infection, never recorded before, with more than 1,440 parasites per single host. Ichoronyssus scutatus, Steatonyssus periblepharus, Vampirolepis balsaci and Molinostrongylus alatus are recorded for the first time in Germany. A checklist for Europe is presented containing records of 98 parasite species of 14 Myotis species. The Myotis myotis from Gladenbach (Hesse, Germany) were parasitized by a diverse parasite fauna with high infestation rates. We assume that in juvenile Myotis the number of parasites is generally higher than in adults due to only later acquired immune competence and behavioural adaptations. Our results revealed new insights into parasite fauna of M. myotis and European bats in general. The

  3. Biogeographic links between southern Atlantic Forest and western South America: Rediscovery, re-description, and phylogenetic relationships of two rare montane anole lizards from Brazil.

    PubMed

    Prates, Ivan; Melo-Sampaio, Paulo Roberto; Drummond, Leandro de Oliveira; Teixeira, Mauro; Rodrigues, Miguel Trefaut; Carnaval, Ana Carolina

    2017-08-01

    Data on species ranges and phylogenetic relationships are key in historical biogeographical inference. In South America, our understanding of the evolutionary processes that underlie biodiversity patterns varies greatly across regions. Little is known, for instance, about the drivers of high endemism in the southern montane region of the Atlantic Rainforest. In this region, former biogeographic connections with other South American ecosystems have been invoked to explain the phylogenetic affinities of a number of endemic taxa. This may also be the case of the montane anole lizards Anolis nasofrontalis and A. pseudotigrinus, known from few specimens collected more than 40years ago. We combine new genetic data with published sequences of species in the Dactyloa clade of Anolis to investigate the phylogenetic relationships of A. nasofrontalis and A. pseudotigrinus, as well as estimate divergence times from their closest relatives. Based on newly sampled and previously overlooked specimens, we provide a taxonomic re-description of those two taxa. Our phylogenetic analysis recovered six main clades within Dactyloa, five of which were previously referred to as species series (aequatorialis, heterodermus, latifrons, punctatus, roquet). A sixth clade clustered A. nasofrontalis and A. pseudotigrinus with A. dissimilis from western Amazonia, A. calimae from the Andes, A. neblininus from the Guiana Shield, and two undescribed Andean taxa. We therefore define a sixth species series within Dactyloa: the neblininus series. Close phylogenetic relationships between highly disjunct, narrowly-distributed anoles suggest that patches of suitable habitat connected the southern Atlantic Forest to western South America during the Miocene, in agreement with the age of former connections between the central Andes and the Brazilian Shield as a result of Andean orogeny. The data also support the view of recurrent evolution (or loss) of a twig anole-like phenotype in mainland anoles, in

  4. Hydrobiological characteristics of Shark River estuary, Everglades National Park, Florida

    USGS Publications Warehouse

    McPherson, B.F.

    1970-01-01

    sardines (Harengula pensacolae) were the dominant animals collected in the higher salinities (10-25 g/l) near the mouth of the estuary. Amphipods (Corophium sp. and Grandidierella sp.), mysids (Mysidopsis almyra and Gastrosaccus dissimilis), crab larvae, and the young anchovies, sardines, and related fish were the dominant forms in the brackish water (1-10 g/l) of the mid-estuary. The presence of large numbers of juvenile and young animals and young animals indicated the importance of these brackish waters as nursery grounds. Aquatic insects, cyclopoid copepods (Macrocyclops sp.), cladocerans, mysids (Taphromysis bowmani), ostracods (Cypridopsis sp. ), fresh-water prawns (Palaemonetes paludosus), and various marshfish were dominant in the ?fresh? headwaters. The amount of plant detritus collected in the estuary averaged about ten times that of the zooplankton. The estimated mean wet-weight of the zooplankton was 65 milligrams per cubic meter (mg/m?) and ranged from 1 to 173 mg/m?, with the smallest amounts occurring in the ?fresh? headwaters. Nekton, consisting of small fish and prawns, ranged from 3 to 214 mg/m? in weight and had a mean of 30 mg/m?. Largest catches were made in the headwaters at the end of the dry season, where the weight of the standing crop increased more than 15 times during the sampling period. The small fish and prawns, which were concentrated in the headwaters at the water level dropped, served as a rich source of food for predatory marine fish and birds.