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Sample records for distancia entre plantas

  1. A Case Study in Distance Learning Systems: Costa Rica's Universidad Estatal a Distancia.

    ERIC Educational Resources Information Center

    Rumble, G. W. S. V.

    A case study of Costa Rica's Universidad Estatal a Distancia, an Open University school with a distance learning system, is presented. Areas of discussion include characteristics of the country, the educational system of Costa Rica, and the university. Specific topics include: the physical setting of Costa Rica; the population; the economy;…

  2. Costa Rica's Universidad Estatal a Distancia: A Case Study. DERG Papers, Number 4.

    ERIC Educational Resources Information Center

    Rumble, Greville

    Costa Rica's Universidad Estatal a Distancia (UNED), the education system in the country, and information on Costa Rica are considered. UNED is helping to equalize geographical inequalities of access to higher education. Overall, UNED's academic programs have been aimed at those who want to obtain professional qualifications. In the first semester…

  3. PREJUICIO Y DISTANCIA SOCIAL HACIA PERSONAS HOMOSEXUALES POR PARTE DE JÓVENES UNIVERSITARIOS

    PubMed Central

    Fernández Rodríguez, María del C.; Squiabro, José Calderón

    2014-01-01

    Se realizó un estudio descriptivo transversal con el propósito de explorar actitudes de rechazo y distancia social hacia las personas gays y lesbianas (GL) en 565 universitarios. Se utilizó una escala para medir Prejuicio y otra escala para medir Distancia Social. Los participantes reflejaron niveles moderados de prejuicio y distancia social (DS) hacia las personas gays y lesbianas. Los varones (M=104.5, DT= 27.47) mostraron significativamente más prejuicio que las mujeres (M=98.8, DT= 23.41). Los hombres (M=22.7, DT= 7.00) mostraron significativamente mayor DS que las mujeres (M=21.1, DT= 5.41). Las personas que asisten con regularidad a la iglesia mostraron más prejuicio y DS que los que no asisten. Se analiza importancia de incluir el tema de la diversidad sexual a través del currículo para desmontar prejuicios hacia la comunidad homosexual. PMID:25606066

  4. Comparisons in the Organisation, Methods, and Results of the Universidad Nacional de Educacion a Distancia (Spain) and The Open University of The United Kingdom.

    ERIC Educational Resources Information Center

    James, Arthur

    The organization, methods, and outcomes of the distance education systems at the Universidad Nacional de Educacion a Distancia (UNED) in Spain and The Open University of the United Kingdom are compared. The following topics are covered: higher education in Spain, UNED's ideology, student characteristics in both universities, organization,…

  5. Hyperthermophilic endoglucanase for in planta lignocellulose conversion

    PubMed Central

    2012-01-01

    Background The enzymatic conversion of lignocellulosic plant biomass into fermentable sugars is a crucial step in the sustainable and environmentally friendly production of biofuels. However, a major drawback of enzymes from mesophilic sources is their suboptimal activity under established pretreatment conditions, e.g. high temperatures, extreme pH values and high salt concentrations. Enzymes from extremophiles are better adapted to these conditions and could be produced by heterologous expression in microbes, or even directly in the plant biomass. Results Here we show that a cellulase gene (sso1354) isolated from the hyperthermophilic archaeon Sulfolobus solfataricus can be expressed in plants, and that the recombinant enzyme is biologically active and exhibits the same properties as the wild type form. Since the enzyme is inactive under normal plant growth conditions, this potentially allows its expression in plants without negative effects on growth and development, and subsequent heat-inducible activation. Furthermore we demonstrate that the recombinant enzyme acts in high concentrations of ionic liquids and can therefore degrade α-cellulose or even complex cell wall preparations under those pretreatment conditions. Conclusion The hyperthermophilic endoglucanase SSO1354 with its unique features is an excellent tool for advanced biomass conversion. Here we demonstrate its expression in planta and the possibility for post harvest activation. Moreover the enzyme is suitable for combined pretreatment and hydrolysis applications. PMID:22928996

  6. Physiology of ex planta nitrogenase activity in Rhizobium japonicum

    SciTech Connect

    Agarwal, A.K.; Keister, D.L.

    1983-05-01

    Thirty-nine wild-type strains of Rhizobium japonicum have been studied for their ability to synthesize nitrogenase ex planta in defined liquid media under microaerobic conditions. Twenty-one produced more than trace amounts of acetylene reduction activity, but only a few of these yielded high activity. The oxygen response curves were similar for most of the nitrogenase-positive strains. The strains derepressible for activity had several phenotypic characteristics different from non-derepressible strains. These included slower growth and lower oxygen consumption under microaerobic conditions and lower extracellular polysaccharide production. Extracellular polysaccharide production during growth on gluconate in every nitrogenase-positive strain assayed was lower under both aerobic and microaerobic conditions than the non-depressible strains. These phenotypic characteristics may be representative of a genotype of a subspecies of R. japonicum. These studies were done in part to enlarge the base number of strains available for studies on the physiology, biochemistry, and genetics of nitrogen fixation. (35 Refs.)

  7. Smallpox subunit vaccine produced in planta confers protection in mice

    PubMed Central

    Golovkin, Maxim; Spitsin, Sergei; Andrianov, Vyacheslav; Smirnov, Yuriy; Xiao, Yuhong; Pogrebnyak, Natalia; Markley, Karen; Brodzik, Robert; Gleba, Yuri; Isaacs, Stuart N.; Koprowski, Hilary

    2007-01-01

    We report here the in planta production of the recombinant vaccinia virus B5 antigenic domain (pB5), an attractive component of a subunit vaccine against smallpox. The antigenic domain was expressed by using efficient transient and constitutive plant expression systems and tested by various immunization routes in two animal models. Whereas oral administration in mice or the minipig with collard-derived insoluble pB5 did not generate an anti-B5 immune response, intranasal administration of soluble pB5 led to a rise of B5-specific immunoglobulins, and parenteral immunization led to a strong anti-B5 immune response in both mice and the minipig. Mice immunized i.m. with pB5 generated an antibody response that reduced virus spread in vitro and conferred protection from challenge with a lethal dose of vaccinia virus. These results indicate the feasibility of producing safe and inexpensive subunit vaccines by using plant production systems. PMID:17428917

  8. Pentahydroxyscirpene—Producing Strains, Formation In Planta, and Natural Occurrence

    PubMed Central

    Varga, Elisabeth; Wiesenberger, Gerlinde; Fruhmann, Philipp; Malachová, Alexandra; Svoboda, Thomas; Lemmens, Marc; Adam, Gerhard; Berthiller, Franz

    2016-01-01

    Trichothecenes are a class of structurally diverse mycotoxins with more than 200 naturally occurring compounds. Previously, a new compound, pentahydroxyscirpene (PHS), was reported as a byproduct of a nivalenol producing Fusarium strain, IFA189. PHS contains a hydroxy group at C-8 instead of the keto group of type B trichothecenes. In this work, we demonstrate that IFA189 belongs to the species Fusarium kyushuense using molecular tools. Production of PHS in vitro was also observed for several isolates of other Fusarium species producing nivalenol. Furthermore, we report the formation of 4-acetyl-PHS by F. kyushuense on inoculated rice. Wheat ears of the variety Remus were infected with IFA189 and the in planta production of PHS was confirmed. Natural occurrence of PHS was verified in barley samples from the Czech Republic using a liquid chromatographic-tandem mass spectrometric method validated for this purpose. Toxicity of PHS to wheat ribosomes was evaluated with a coupled in vitro transcription and translation assay, which showed that PHS inhibits protein biosynthesis slightly less than nivalenol and deoxynivalenol. PMID:27754401

  9. Time Gating of Chloroplast Autofluorescence Allows Clearer Fluorescence Imaging In Planta

    PubMed Central

    Kodama, Yutaka

    2016-01-01

    Chloroplast, an organelle facilitating photosynthesis, exhibits strong autofluorescence, which is an undesired background signal that restricts imaging experiments with exogenous fluorophore in plants. In this study, the autofluorescence was characterized in planta under confocal laser microscopy, and it was found that the time-gated imaging technique completely eliminates the autofluorescence. As a demonstration of the technique, a clearer signal of fluorescent protein-tagged phototropin, a blue-light photoreceptor localized at the chloroplast periphery, was visualized in planta. PMID:27027881

  10. Antifreeze Proteins Modify the Freezing Process In Planta12

    PubMed Central

    Griffith, Marilyn; Lumb, Chelsey; Wiseman, Steven B.; Wisniewski, Michael; Johnson, Robert W.; Marangoni, Alejandro G.

    2005-01-01

    During cold acclimation, winter rye (Secale cereale L. cv Musketeer) plants accumulate antifreeze proteins (AFPs) in the apoplast of leaves and crowns. The goal of this study was to determine whether these AFPs influence survival at subzero temperatures by modifying the freezing process or by acting as cryoprotectants. In order to inhibit the growth of ice, AFPs must be mobile so that they can bind to specific sites on the ice crystal lattice. Guttate obtained from cold-acclimated winter rye leaves exhibited antifreeze activity, indicating that the AFPs are free in solution. Infrared video thermography was used to observe freezing in winter rye leaves. In the absence of an ice nucleator, AFPs had no effect on the supercooling temperature of the leaves. However, in the presence of an ice nucleator, AFPs lowered the temperature at which the leaves froze by 0.3°C to 1.2°C. In vitro studies showed that apoplastic proteins extracted from cold-acclimated winter rye leaves inhibited the recrystallization of ice and also slowed the rate of migration of ice through solution-saturated filter paper. When we examined the possible role of winter rye AFPs in cryoprotection, we found that lactate dehydrogenase activity was higher after freezing in the presence of AFPs compared with buffer, but the same effect was obtained by adding bovine serum albumin. AFPs had no effect on unstacked thylakoid volume after freezing, but did inhibit stacking of the thylakoids, thus indicating a loss of thylakoid function. We conclude that rye AFPs have no specific cryoprotective activity; rather, they interact directly with ice in planta and reduce freezing injury by slowing the growth and recrystallization of ice. PMID:15805474

  11. Effets Josephson generalises entre antiferroaimants et entre supraconducteurs antiferromagnetiques

    NASA Astrophysics Data System (ADS)

    Chasse, Dominique

    L'effet Josephson est generalement presente comme le resultat de l'effet tunnel coherent de paires de Cooper a travers une jonction tunnel entre deux supraconducteurs, mais il est possible de l'expliquer dans un contexte plus general. Par exemple, Esposito & al. ont recemment demontre que l'effet Josephson DC peut etre decrit a l'aide du boson pseudo-Goldstone de deux systemes couples brisant chacun la symetrie abelienne U(1). Puisque cette description se generalise de facon naturelle a des brisures de symetries continues non-abeliennes, l'equivalent de l'effet Josephson devrait donc exister pour des types d'ordre a longue portee differents de la supraconductivite. Le cas de deux ferroaimants itinerants (brisure de symetrie 0(3)) couples a travers une jonction tunnel a deja ete traite dans la litterature Afin de mettre en evidence la generalite du phenomene et dans le but de faire des predictions a partir d'un modele realiste, nous etudions le cas d'une jonction tunnel entre deux antiferroaimants itinerants. En adoptant une approche Similaire a celle d'Ambegaokar & Baratoff pour une jonction Josephson, nous trouvons un courant d'aimantation alternee a travers la jonction qui est proportionnel a sG x sD ou fG et sD sont les vecteurs de Neel de part et d'autre de la jonction. La fonction sinus caracteristique du courant Josephson standard est donc remplacee.ici par un produit vectoriel. Nous montrons que, d'un point de vue microscopique, ce phenomene resulte de l'effet tunnel coherent de paires particule-trou de spin 1 et de vecteur d'onde net egal au vecteur d'onde antiferromagnetique Q. Nous trouvons egalement la dependance en temperature de l'analogue du courant critique. En presence d'un champ magnetique externe, nous obtenons l'analogue de l'effet Josephson AC et la description complete que nous en donnons s'applique aussi au cas d'une jonction tunnel entre ferroaimants (dans ce dernier cas, les traitements anterieurs de cet effet AC s'averent incomplets). Nous

  12. Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry

    PubMed Central

    2012-01-01

    Understanding protein and gene function requires identifying interaction partners using biochemical, molecular or genetic tools. In plants, searching for novel protein-protein interactions is limited to protein purification assays, heterologous in vivo systems such as the yeast-two-hybrid or mutant screens. Ideally one would be able to search for novel protein partners in living plant cells. We demonstrate that it is possible to screen for novel protein-protein interactions from a random library in protoplasted Arabidopsis plant cells and recover some of the interacting partners. Our screen is based on capturing the bi-molecular complementation of mYFP between an YN-bait fusion partner and a completely random prey YC-cDNA library with FACS. The candidate interactions were confirmed using in planta BiFC assays and in planta FRET-FLIM assays. From this work, we show that the well characterized protein Calcium Dependent Protein Kinase 3 (CPK3) interacts with APX3, HMGB5, ORP2A and a ricin B-related lectin domain containing protein At2g39050. This is one of the first randomin planta screens to be successfully employed. PMID:22789293

  13. Highly Efficient Agrobacterium-Mediated Transformation of Wheat Via In Planta Inoculation

    NASA Astrophysics Data System (ADS)

    Risacher, Thierry; Craze, Melanie; Bowden, Sarah; Paul, Wyatt; Barsby, Tina

    This chapter details a reproducible method for the transformation of spring wheat using Agrobacterium tumefaciens via the direct inoculation of bacteria into immature seeds in planta as described in patent WO 00/63398(1. Transformation efficiencies from 1 to 30% have been obtained and average efficiencies of at least 5% are routinely achieved. Regenerated plants are phenotypically normal with 30-50% of transformation events carrying introduced genes at single insertion sites, a higher rate than is typically reported for transgenic plants produced using biolistic transformation methods.

  14. In planta cloning of geminiviral DNA: the true Sida micrantha mosaic virus.

    PubMed

    Jeske, Holger; Gotthardt, Diether; Kober, Sigrid

    2010-02-01

    The circular single-stranded DNAs of geminiviruses are multiplied efficiently and preferentially by rolling circle amplification (RCA), and can be diagnosed readily by restriction fragment length polymorphism (RFLP) and direct sequencing of the RCA product. Two strategies are described for cloning geminiviruses from plants harboring mixed infections by using RCA and RFLP with plant-derived nucleic acids without the need for bacterial amplification. By combining both these approaches, the true Sida micrantha mosaic virus was identified. The advantages of maintaining the quasispecies nature of a virus during in planta cloning is discussed with respect to reliable virus identification and resistance breeding.

  15. i Planta

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This application was designed for farmers, agriculture researchers, and gardeners. The application includes a calculator, a data logger, and a GPS mapping function. It was designed for vegetable farming but can be used for open field applications. The calculator solves for the amount of pesticide,...

  16. Prediction of the in planta P. pachyrhizi secretome and potential effector families.

    PubMed

    De Carvalho, Mayra C da C G; Nascimento, Leandro Costa; Darben, Luana M; Polizel-Podanosqui, Adriana M; Lopes-Caitar, Valéria S; Qi, Mingsheng; Rocha, Carolina S; Carazzolle, Marcelo Falsarella; Kuwahara, Márcia K; Pereira, Goncalo A G; Abdelnoor, Ricardo V; Whitham, Steven A; Marcelino-Guimarães, Francismar C

    2016-03-24

    Asian soybean rust (ASR) caused by the obligate biotrophic fungus Phakopsora pachyrhizi can cause losses greater than 80%. Despite its economic importance, there is no soybean cultivar with durable ASR resistance (Goellner et al., 2010). In addition, the P. pachyrhizi genome is not yet available. However, the availability of other rust genomes as well as the development of sample enrichment strategies and bioinformatics tools has improved our knowledge of the ASR secretome and its potential effectors. In this context, we used a combination of laser capture microdissection (LCM), RNAseq and a bioinformatics pipeline to identify a total of 36,350 P. pachyrhizi contigs expressed in planta and a predicted secretome of 851 proteins. Some of the predicted secreted proteins had characteristics of candidate effectors, including small size, cysteine rich, do not contain PFAM domains (except those associated with pathogenicity), and were strongly expressed in planta. A comparative analysis of the predicted secreted proteins present in Pucciniales species identified new members of soybean rust and new Pucciniales- or P. pachyrhizi-specific families (tribes). Members of some families were strongly upregulated during early infection, starting with initial infection through haustorium formation. Effector candidates selected from two of these families were able to suppress immunity in transient assays, and were localized in the plant cytoplasm and nuclei. These experiments support our bioinformatics predictions and show that these families contain members that have functions consistent with P. pachyrhizi effectors. This article is protected by copyright. All rights reserved.

  17. Inhibition of fungal and bacterial plant pathogens in vitro and in planta with ultrashort cationic lipopeptides.

    PubMed

    Makovitzki, Arik; Viterbo, Ada; Brotman, Yariv; Chet, Ilan; Shai, Yechiel

    2007-10-01

    Plant diseases constitute an emerging threat to global food security. Many of the currently available antimicrobial agents for agriculture are highly toxic and nonbiodegradable and cause extended environmental pollution. Moreover, an increasing number of phytopathogens develop resistance to them. Recently, we have reported on a new family of ultrashort antimicrobial lipopeptides which are composed of only four amino acids linked to fatty acids (A. Makovitzki, D. Avrahami, and Y. Shai, Proc. Natl. Acad. Sci. USA 103:15997-16002, 2006). Here, we investigated the activities in vitro and in planta and the modes of action of these short lipopeptides against plant-pathogenic bacteria and fungi. They act rapidly, at low micromolar concentrations, on the membranes of the microorganisms via a lytic mechanism. In vitro microscopic analysis revealed wide-scale damage to the microorganism's membrane, in addition to inhibition of pathogen growth. In planta potent antifungal activity was demonstrated on cucumber fruits and leaves infected with the pathogen Botrytis cinerea as well as on corn leaves infected with Cochliobolus heterostrophus. Similarly, treatment with the lipopeptides of Arabidopsis leaves infected with the bacterial leaf pathogen Pseudomonas syringae efficiently and rapidly reduced the number of bacteria. Importantly, in contrast to what occurred with many native lipopeptides, no toxicity was observed on the plant tissues. These data suggest that the ultrashort lipopeptides could serve as native-like antimicrobial agents economically feasible for use in plant protection.

  18. Bcmimp1, a Botrytis cinerea Gene Transiently Expressed in planta, Encodes a Mitochondrial Protein

    PubMed Central

    Benito-Pescador, David; Santander, Daniela; Arranz, M.; Díaz-Mínguez, José M.; Eslava, Arturo P.; van Kan, Jan A. L.; Benito, Ernesto P.

    2016-01-01

    Botrytis cinerea is a widespread necrotrophic fungus which infects more than 200 plant species. In an attempt to characterize the physiological status of the fungus in planta and to identify genetic factors contributing to its ability to infect the host cells, a differential gene expression analysis during the interaction B. cinerea-tomato was carried out. Gene Bcmimp1 codes for a mRNA detected by differential display in the course of this analysis. During the interaction with the host, it shows a transient expression pattern with maximal expression levels during the colonization and maceration of the infected tissues. Bioinformatic analysis suggested that BCMIMP1 is an integral membrane protein located in the mitochondrial inner membrane. Co-localization experiments with a BCMIMP1-GFP fusion protein confirmed that the protein is targeted to the mitochondria. ΔBcmimp1 mutants do not show obvious phenotypic differences during saprophytic growth and their infection ability was unaltered as compared to the wild-type. Interestingly, the mutants produced increased levels of reactive oxygen species, likely as a consequence of disturbed mitochondrial function. Although Bcmimp1 expression is enhanced in planta it cannot be considered a pathogenicity factor. PMID:26952144

  19. Dynamic root exudation of sorgoleone and its in planta mechanism of action

    PubMed Central

    Dayan, Franck E.; Howell, J'Lynn; Weidenhamer, Jeffrey D.

    2009-01-01

    The oily droplets exuded from the root hairs of sorghum are composed of a 1:1 ratio of sorgoleone and its lipid resorcinol analogue. The production of these droplets appears to be suppressed when c. 20 μg of exudate mg−1 root dry weight accumulates at the tip of the root hairs. However, more exudate is produced following gentle washing of the roots with water, suggesting that the biosynthesis of lipid benzoquinones and resorcinols is a dynamic process. Sorgoleone interferes with several molecular target sites, including photosynthetic electron transport, in in vitro assays. However, the in planta mechanism of action of sorgoleone remains controversial because it is not clear whether this lipid benzoquinone exuding from the roots of sorghum is taken up by roots of the receiving plants and translocated to their foliage where it must enter the chloroplast and inhibit PSII in the thylakoid membrane. Experiments designed to test the in planta mode of action of sorgoleone demonstrated that it has no effect on the photosynthesis of older plants, but inhibits photosynthesis in germinating seedlings. Sorgoleone is not translocated acropetally in older plants, but can be absorbed through the hypocotyl and cotyledonary tissues. Therefore, the mode of action of sorgoleone may be the result of inhibition of photosynthesis in young seedlings in concert with inhibition of its other molecular target sites in older plants. PMID:19357432

  20. A method for obtaining RNA from Hemileia vastatrix appressoria produced in planta, suitable for transcriptomic analyses.

    PubMed

    Loureiro, Andreia; Gil Azinheira, Helena; do Céu Silva, Maria; Talhinhas, Pedro

    2015-11-01

    Appressoria are the first infection structures developed by rust fungi and require specific topographic signals from the host for their differentiation. The ease in obtaining appressoria in vitro for these biotrophic fungi led to studies concerning gene expression and gene discovery at appressorial level, avoiding the need to distinguish plant and fungal transcripts. However, in some pathosystems, it was observed that gene expression in appressoria seems to be influenced by host-derived signals, suggesting that transcriptomic analyses performed from in planta differentiated appressoria would be potentially more informative than those from in vitro differentiated appressoria. Nevertheless analysing appressorial RNA obtained from in planta samples is often hampered by an excessive dilution of fungal RNA within plant RNA, besides uncertainty regarding the fungal or plant origin of RNA from highly conserved genes. To circumvent these difficulties, we have recovered Hemileia vastatrix appressoria from Arabica coffee leaf surface using a film of nitrocellulose dissolved in butyl and ethyl acetates (nail polish), and extracted fungal RNA from the polish peel. RNA thus obtained is of good quality and usable for cDNA synthesis and transcriptomic (quantitative PCR) studies. This method could provide the means to investigate specific host-induced appressoria-related fungal pathogenicity factors.

  1. Ethylene Production by Pseudomonas syringae Pathovars In Vitro and In Planta

    PubMed Central

    Weingart, H.; Volksch, B.

    1997-01-01

    Significant amounts of ethylene were produced by Pseudomonas syringae pv. glycinea, pv. phaseolicola (which had been isolated from viny weed Pueraria lobata [Willd.] Ohwi [common name, kudzu]), and pv. pisi in synthetic medium. On the other hand, the bean strains of P. syringae pv. phaseolicola and strains of 17 other pathovars did not produce ethylene. P. syringae pv. glycinea and P. syringae pv. phaseolicola produced nearly identical levels of ethylene (about 5 x 10(sup-7) nl h(sup-1) cell(sup-1)), which were about 10 times higher than the ethylene level of P. syringae pv. pisi. Two 22-bp oligonucleotide primers derived from the ethylene-forming enzyme (efe) gene of P. syringae pv. phaseolicola PK2 were investigated for their ability to detect ethylene-producing P. syringae strains by PCR analysis. PCR amplification with this primer set resulted in a specific 0.99-kb fragment in all ethylene-producing strains with the exception of the P. syringae pv. pisi strains. Therefore, P. syringae pv. pisi may use a different biosynthetic pathway for ethylene production or the sequence of the efe gene is less conserved in this bacterium. P. syringae pv. phaseolicola isolated from kudzu and P. syringae pv. glycinea also produced ethylene in planta. It could be shown that the enhanced ethylene production in diseased tissue was due to the production of ethylene by the inoculated bacteria. Ethylene production in vitro and in planta was strictly growth associated. PMID:16535480

  2. In planta production and characterization of a hyperthermostable GH10 xylanase in transgenic sugarcane.

    PubMed

    Kim, Jae Yoon; Nong, Guang; Rice, John D; Gallo, Maria; Preston, James F; Altpeter, Fredy

    2017-03-01

    Sugarcane (Saccharum sp. hybrids) is one of the most efficient and sustainable feedstocks for commercial production of fuel ethanol. Recent efforts focus on the integration of first and second generation bioethanol conversion technologies for sugarcane to increase biofuel yields. This integrated process will utilize both the cell wall bound sugars of the abundant lignocellulosic sugarcane residues in addition to the sucrose from stem internodes. Enzymatic hydrolysis of lignocellulosic biomass into its component sugars requires significant amounts of cell wall degrading enzymes. In planta production of xylanases has the potential to reduce costs associated with enzymatic hydrolysis but has been reported to compromise plant growth and development. To address this problem, we expressed a hyperthermostable GH10 xylanase, xyl10B in transgenic sugarcane which displays optimal catalytic activity at 105 °C and only residual catalytic activity at temperatures below 70 °C. Transgene integration and expression in sugarcane were confirmed by Southern blot, RT-PCR, ELISA and western blot following biolistic co-transfer of minimal expression cassettes of xyl10B and the selectable neomycin phosphotransferase II. Xylanase activity was detected in 17 transgenic lines with a fluorogenic xylanase activity assay. Up to 1.2% of the total soluble protein fraction of vegetative progenies with integration of chloroplast targeted expression represented the recombinant Xyl10B protein. Xyl10B activity was stable in vegetative progenies. Tissues retained 75% of the xylanase activity after drying of leaves at 35 °C and a 2 month storage period. Transgenic sugarcane plants producing Xyl10B did not differ from non-transgenic sugarcane in growth and development under greenhouse conditions. Sugarcane xylan and bagasse were used as substrate for enzymatic hydrolysis with the in planta produced Xyl10B. TLC and HPLC analysis of hydrolysis products confirmed the superior catalytic activity

  3. Mexican papita viroid and tomato planta macho viroid belong to a single species in the genus Pospiviroid

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Tomato planta macho viroid (TPMVd) and Mexican papita viroid (MPVd) are two closely-related (>90% sequence identity) members of the genus Pospiviroid. Their current status as separate species is based upon the reported ability of TPMVd to replicate in Gomphrena globosa and the inability of this viro...

  4. In planta transformation method for T-DNA transfer in orchids

    NASA Astrophysics Data System (ADS)

    Semiarti, Endang; Purwantoro, Aziz; Mercuriani, Ixora S.; Anggriasari, Anida M.; Jang, Seonghoe; Suhandono, Sony; Machida, Yasunori; Machida, Chiyoko

    2014-03-01

    Transgenic plant technology is an efficient tool to study the function of gene(s) in plant. The most popular and widely used technique is Agrobacterium-mediated transformation in which cocultivation was done by immersing the plant tissues/organ in overnight bacterial cultured for about 30 minutes to one hour under in vitro condition. In this experiment, we developed more easier technique that omitted the in vitro step during cocultivation with Agrobacterium, namely in planta transformation method. Pollinaria (compact pollen mass of orchid) of Phalaenopsis amabilis and Spathoglottis plicata orchids were used as target explants that were immersed into bacterial culture for 30 minutes, then dried up the pollinaria, the transformed pollinaria was used to pollinate orchid flowers. The T-DNA used for this experiments were Ubipro∷PaFT/A. tumefaciens GV3101 for P. amabilis and MeEF1α2 pro∷GUS/ A. tumefaciens LBA 4404 for S.plicata. Seeds that were produced from pollinated flowers were grown onto 10 mg/l hygromicin containing NP (New Phalaenopsis) medium. The existance of transgene in putative transformant protocorm (developing orchid embryo) genome was confirmed using PCR with specific primers of either PaFT or GUS genes. Histochemical GUS assay was also performed to the putative transformants. The result showed that transformation frequencies were 2.1 % in P. amabilis, and 0,53% in S. plicata. These results indicates that in planta transformation method could be used for Agrobacterium-mediated genetic transformation, with advantage easier and more secure work from contaminants than that of the in vitro method.

  5. In Planta Localization of Stilbenes within Picea abies Phloem1[OPEN

    PubMed Central

    Kuroda, Katsushi; Pranovich, Andrey; Roig-Juan, Sílvia; Aoki, Dan

    2016-01-01

    Phenolic stilbene glucosides (astringin, isorhapontin, and piceid) and their aglycons commonly accumulate in the phloem of Norway spruce (Picea abies). However, current knowledge about the localization and accumulation of stilbenes within plant tissues and cells remains limited. Here, we used an innovative combination of novel microanalytical techniques to evaluate stilbenes in a frozen-hydrated condition (i.e. in planta) and a freeze-dried condition across phloem tissues. Semiquantitative time-of-flight secondary ion-mass spectrometry imaging in planta revealed that stilbenes were localized in axial parenchyma cells. Quantitative gas chromatography analysis showed the highest stilbene content in the middle of collapsed phloem with decreases toward the outer phloem. The same trend was detected for soluble sugar and water contents. The specimen water content may affect stilbene composition; the glucoside-to-aglycon ratio decreased slightly with decreases in water content. Phloem chemistry was correlated with three-dimensional structures of phloem as analyzed by microtomography. The outer phloem was characterized by a high volume of empty parenchyma, reduced ray volume, and a large number of axial parenchyma with porous vacuolar contents. Increasing porosity from the inner to the outer phloem was related to decreasing compactness of stilbenes and possible secondary oxidation or polymerization. Our results indicate that aging-dependent changes in phloem may reduce cell functioning, which affects the capacity of the phloem to store water and sugar, and may reduce the defense potential of stilbenes in the axial parenchyma. Our results highlight the power of using a combination of techniques to evaluate tissue- and cell-level mechanisms involved in plant secondary metabolite formation and metabolism. PMID:27531441

  6. Native-sized spider silk proteins synthesized in planta via intein-based multimerization.

    PubMed

    Hauptmann, Valeska; Weichert, Nicola; Menzel, Matthias; Knoch, Dominic; Paege, Norman; Scheller, Jürgen; Spohn, Uwe; Conrad, Udo; Gils, Mario

    2013-04-01

    The synthesis of native-sized proteins is a pre-requisite for exploiting the potential of spider silk as a bio-based material. The unique properties of spider silk, such as extraordinary tensile strength and elasticity, result from the highly repetitive nature of spider silk protein motifs. The present report describes the combination of spider silk flagelliform protein (FLAG) production in the endoplasmic reticulum of tobacco plant leaf cells with an intein-based posttranslational protein fusion technology. The repeated ligation of FLAG monomers resulted in the formation of large multimers. This method avoids the need for highly repetitive transgenes, which may result in a higher genetic and transcriptional stability. Here we show, for the first time, the production of synthetic, high molecular weight spider silk proteins larger than 250 kDa based on the assembly of protein monomers via intein-mediated trans-splicing in planta. The resulting multimeric structures form microfibers, thereby demonstrating their great potential as a biomaterial.

  7. Root internalization, transport and in-planta survival of Salmonella enterica serovar Newport in sweet basil.

    PubMed

    Gorbatsevich, Elena; Sela Saldinger, Shlomo; Pinto, Riky; Bernstein, Nirit

    2013-02-01

    It is now acknowledged that food-borne pathogens present in the irrigation water or soil can become associated with crop plants in the field, penetrate internal plant tissues via the root, translocate and survive inside plants. Only little information is available concerning interaction between enteric pathogens and plants. The present study evaluated the potential for contamination of the aromatic plant, sweet basil during cultivation, by Salmonella enterica serovar Newport. Root internalization was plant-age-dependent, with the highest susceptibility occurring at the beginning of the rapid growth phase of the root. Higher incidence of internalization was detected in vegetative than reproductive plant organs, pointing at bacterial transport in the transpiration stream. Internalized Salmonella survived only < 30 h in the phyllosphere. In contrast, survival of Salmonella on the leaf surface was much pronounced (at least 8 days), and the initial decay rate was lower at the abaxial (lower) compared with the adaxial (upper) side of the leaf. Although the experiments were conducted with high concentration of Salmonella unlikely to happen in the field, internalization occurred at a low frequency and in-planta survival was limited to less than 30 h. These findings imply that leaf surface contamination, rather than root internalization, may pose higher risk for human infection following consumption of contaminated basil.

  8. In planta production of flock house virus transencapsidated RNA and its potential use as a vaccine.

    PubMed

    Zhou, Yiyang; Maharaj, Payal D; Mallajosyula, Jyothi K; McCormick, Alison A; Kearney, Christopher M

    2015-04-01

    We have developed a transencapsidated vaccine delivery system based on the insect virus, Flock House virus (FHV). FHV is attractive due to its small genome size, simple organization, and nonpathogenic characteristics. With the insertion of a Tobacco mosaic virus (TMV) origin of assembly (Oa), the independently replicating FHV RNA1 can be transencapsidated by TMV coat protein. In this study, we demonstrated that the Oa-adapted FHV RNA1 transencapsidation process can take place in planta, by using a bipartite plant expression vector system, where TMV coat protein is expressed by another plant virus vector, Foxtail mosaic virus (FoMV). Dual infection in the same cell by both FHV and FoMV was observed. Though an apparent classical coat protein-mediated resistance repressed FHV expression, this was overcome by delaying inoculation of the TMV coat protein vector by 3 days after FHV vector inoculation. Expression of the transgene marker in animals by these in vivo-generated transencapsidated nanoparticles was confirmed by mouse vaccination, which also showed an improved vaccine response compared to similar in vitro-produced vaccines.

  9. In Planta Determination of the mRNA-Binding Proteome of Arabidopsis Etiolated Seedlings.

    PubMed

    Reichel, Marlene; Liao, Yalin; Rettel, Mandy; Ragan, Chikako; Evers, Maurits; Alleaume, Anne-Marie; Horos, Rastislav; Hentze, Matthias W; Preiss, Thomas; Millar, Anthony A

    2016-10-01

    RNA binding proteins (RBPs) control the fate and expression of a transcriptome. Despite this fundamental importance, our understanding of plant RBPs is rudimentary, being mainly derived via bioinformatic extrapolation from other kingdoms. Here, we adapted the mRNA-protein interactome capture method to investigate the RNA binding proteome in planta. From Arabidopsis thaliana etiolated seedlings, we captured more than 700 proteins, including 300 with high confidence that we have defined as the At-RBP set. Approximately 75% of these At-RBPs are bioinformatically linked with RNA biology, containing a diversity of canonical RNA binding domains (RBDs). As no prior experimental RNA binding evidence exists for the majority of these proteins, their capture now authenticates them as RBPs. Moreover, we identified protein families harboring emerging and potentially novel RBDs, including WHIRLY, LIM, ALBA, DUF1296, and YTH domain-containing proteins, the latter being homologous to animal RNA methylation readers. Other At-RBP set proteins include major signaling proteins, cytoskeleton-associated proteins, membrane transporters, and enzymes, suggesting the scope and function of RNA-protein interactions within a plant cell is much broader than previously appreciated. Therefore, our foundation data set has provided an unbiased insight into the RNA binding proteome of plants, on which future investigations into plant RBPs can be based.

  10. Advances in alfalfa mosaic virus-mediated expression of anthrax antigen in planta

    SciTech Connect

    Brodzik, R.; Bandurska, K.; Deka, D.; Golovkin, M.; Koprowski, H. . E-mail: h_koprowski@jefferson.edu

    2005-12-16

    Plant viruses show great potential for production of pharmaceuticals in plants. Such viruses can harbor a small antigenic peptide(s) as a part of their coat proteins (CP) and elicit an antigen-specific immune response. Here, we report the high yield and consistency in production of recombinant alfalfa mosaic virus (AlMV) particles for specific presentation of the small loop 15 amino acid epitope from domain-4 of the Bacillus anthracis protective antigen (PA-D4s). The epitope was inserted immediately after the first 25 N-terminal amino acids of AlMV CP to retain genome activation and binding of CP to viral RNAs. Recombinant AlMV particles were efficiently produced in tobacco, easily purified for immunological analysis, and exhibited extended stability and systemic proliferation in planta. Intraperitional injections of mice with recombinant plant virus particles harboring the PA-D4s epitope elicited a distinct immune response. Western blotting and ELISA analysis showed that sera from immunized mice recognized both native PA antigen and the AlMV CP.

  11. TYLCV-Is movement in planta does not require V2 protein

    SciTech Connect

    Hak, Hagit; Levy, Yael; Chandran, Sam A.; Belausov, Eduard; Loyter, Abraham; Lapidot, Moshe; Gafni, Yedidya

    2015-03-15

    Tomato yellow leaf curl virus (TYLCV), a major tomato pathogen causing extensive crop losses, is a whitefly-transmitted geminivirus. V2 mutants of TYLCV-Is and related viruses tend to induce symptomless infection with attenuated viral DNA levels, while accumulating close to wild-type DNA levels in protoplasts, suggesting V2 as a movement protein. The discovery of plant-silencing mechanisms and viral silencing suppressors, V2 included, led us to reconsider V2's involvement in viral movement. We studied two mutant versions of the virus, one impaired in V2 silencing-suppression activity, and another carrying a non-translatable V2. While both mutant viruses spread in the infected plant to newly emerged leaves at the same rate as the wild-type virus, their DNA-accumulation levels were tenfold lower than in the wild-type virus. Thus, we suggest that the setback in virus proliferation, previously ascribed to a movement impediment, is due to lack of silencing-suppression activity. - Highlights: • TYLCV-Is V2 protein is localized in distinct microbodies throughout the cell cytoplasm, around the nucleus and in association with cytoplasmic strands but is not associated with the plasmodesmata. • Disruption of RNA-silencing suppression activity of TYLCV-Is V2 protein causes low titer of the virus in the infected plants. • The movement of TYLCV-Is in planta does not require a functional V2 protein.

  12. Assessing environmental impacts of genetically modified plants on non-target organisms: The relevance of in planta studies.

    PubMed

    Arpaia, Salvatore; Birch, A Nicholas E; Kiss, Jozsef; van Loon, Joop J A; Messéan, Antoine; Nuti, Marco; Perry, Joe N; Sweet, Jeremy B; Tebbe, Christoph C

    2017-04-01

    In legal frameworks worldwide, genetically modified plants (GMPs) are subjected to pre-market environmental risk assessment (ERA) with the aim of identifying potential effects on the environment. In the European Union, the EFSA Guidance Document introduces the rationale that GMPs, as well as their newly produced metabolites, represent the potential stressor to be evaluated during ERA. As a consequence, during several phases of ERA for cultivation purposes, it is considered necessary to use whole plants or plant parts in experimental protocols. The importance of in planta studies as a strategy to address impacts of GMPs on non-target organisms is demonstrated, to evaluate both effects due to the intended modification in plant phenotype (e.g. expression of Cry proteins) and effects due to unintended modifications in plant phenotype resulting from the transformation process (e.g. due to somaclonal variations or pleiotropic effects). In planta tests are also necessary for GMPs in which newly expressed metabolites cannot easily be studied in vitro. This paper reviews the scientific literature supporting the choice of in planta studies as a fundamental tool in ERA of GMPs in cultivation dossiers; the evidence indicates they can realistically mimic the ecological relationships occurring in their receiving environments and provide important insights into the biology and sustainable management of GMPs.

  13. Assessment of factors influencing the Agrobacterium-mediated in planta seed transformation of brinjal (Solanum melongena L.).

    PubMed

    Subramanyam, Kondeti; Rajesh, Manoharan; Jaganath, Balusamy; Vasuki, Amirthalingam; Theboral, Jeevaraj; Elayaraja, Dhandapani; Karthik, Sivabalan; Manickavasagam, Markandan; Ganapathi, Andy

    2013-09-01

    An efficient and reproducible in planta transformation method was developed for brinjal using seed as an explant. The brinjal seeds were infected with Agrobacterium tumefaciens EHA 105 harbouring pCAMBIA 1301-bar plasmid, and the transformants were selected against BASTA®. Several parameters influencing the in planta seed transformation such as pre-culture duration, acetosyringone concentration, surfactants, duration of sonication, vacuum pressure and vacuum duration have been evaluated. The putatively transformed (T 0) brinjal plants were screened by GUS histochemical analysis. Among the different combinations and concentrations tested, when the 18-h pre-cultured brinjal seeds were sonicated for 20 min and vacuum infiltered for 3 min at 500 mm of Hg in Agrobacterium suspension containing 100 μM acetosyringone, 0.2 % Silwett L-77 favoured the Agrobacterium infection and showed maximum transformation efficiency. Among the five brinjal varieties evaluated, Arka Samhitha showed maximum transformation efficiency at 45.66 %. The transgene was successfully transmitted to progeny plants (T 1) which was evidenced by GUS histochemical analysis, polymerase chain reaction and Southern hybridisation. The in planta protocol developed in the present study would be beneficial to transfer the economically and nutritionally important genes into different varieties of brinjal, and the transgenic brinjal plants can be produced in less time (approximately 27 days).

  14. A real-time fluorogenic assay for the visualization of glycoside hydrolase activity in planta.

    PubMed

    Ibatullin, Farid M; Banasiak, Alicja; Baumann, Martin J; Greffe, Lionel; Takahashi, Junko; Mellerowicz, Ewa J; Brumer, Harry

    2009-12-01

    There currently exists a diverse array of molecular probes for the in situ localization of polysaccharides, nucleic acids, and proteins in plant cells, including reporter enzyme strategies (e.g. protein-glucuronidase fusions). In contrast, however, there is a paucity of methods for the direct analysis of endogenous glycoside hydrolases and transglycosidases responsible for cell wall remodeling. To exemplify the potential of fluorogenic resorufin glycosides to address this issue, a resorufin beta-glycoside of a xylogluco-oligosaccharide (XXXG-beta-Res) was synthesized as a specific substrate for in planta analysis of XEH activity. The resorufin aglycone is particularly distinguished for high sensitivity in muro assays due to a low pK(a) (5.8) and large extinction coefficient (epsilon 62,000 M(-1) cm(-1)), long-wavelength fluorescence (excitation 571 nm/emission 585 nm), and high quantum yield (0.74) of the corresponding anion. In vitro analyses demonstrated that XXXG-beta-Res is hydrolyzed by the archetypal plant XEH, nasturtium (Tropaeolum majus) NXG1, with classical Michaelis-Menten substrate saturation kinetics and a linear dependence on both enzyme concentration and incubation time. Further, XEH activity could be visualized in real time by observing the localized increase in fluorescence in germinating nasturtium seeds and Arabidopsis (Arabidopsis thaliana) inflorescent stems by confocal microscopy. Importantly, this new in situ XEH assay provides an essential complement to the in situ xyloglucan endotransglycosylase assay, thus allowing delineation of the disparate activities encoded by xyloglucan endotransglycosylase/hydrolase genes directly in plant tissues. The observation that XXXG-beta-Res is also hydrolyzed by diverse microbial XEHs indicates that this substrate, and resorufin glycosides in general, may find broad applicability for the analysis of wall restructuring by polysaccharide hydrolases during morphogenesis and plant-microbe interactions.

  15. Bioinspired engineering study of Plantae vascules for self-healing composite structures.

    PubMed

    Trask, R S; Bond, I P

    2010-06-06

    This paper presents the first conceptual study into creating a Plantae-inspired vascular network within a fibre-reinforced polymer composite laminate, which provides an ongoing self-healing functionality without incurring a mass penalty. Through the application of a 'lost-wax' technique, orthogonal hollow vascules, inspired by the 'ray cell' structures found in ring porous hardwoods, were successfully introduced within a carbon fibre-reinforced epoxy polymer composite laminate. The influence on fibre architecture and mechanical behaviour of single vascules (located on the laminate centreline) when aligned parallel and transverse to the local host ply was characterized experimentally using a compression-after-impact test methodology. Ultrasonic C-scanning and high-resolution micro-CT X-ray was undertaken to identify the influence of and interaction between the internal vasculature and impact damage. The results clearly show that damage morphology is influenced by vascule orientation and that a 10 J low-velocity impact damage event is sufficient to breach the vasculature; a prerequisite for any subsequent self-healing function. The residual compressive strength after a 10 J impact was found to be dependent upon vascule orientation. In general, residual compressive strength decreased to 70 per cent of undamaged strength when vasculature was aligned parallel to the local host ply and a value of 63 per cent when aligned transverse. This bioinspired engineering study has illustrated the potential that a vasculature concept has to offer in terms of providing a self-healing function with minimum mass penalty, without initiating premature failure within a composite structure.

  16. Cytokine changes in gastric and colonic epithelial cell in response to Planta ovata extract.

    PubMed

    Yakoob, Javed; Jafri, Wasim; Mehmood, Malik Hassan; Abbas, Zaigham; Tariq, Kanwal

    2017-03-22

    Background Psyllium (Planta ovata, Ispaghul) seed and husk are used for treatment of altered bowel habit, i. e. constipation and diarrhea. We studied the effect of Ispaghul extract on secretion of interleukin-1 beta (IL-1β) by AGS (ATCC CRL 1739) and SW480 (ATCC CCL-227) epithelial cell lines and determined whether Ispaghul extract has an effect on IL-1β secretion by Helicobacter pylori (H. pylori)-stimulated AGS cell and Escherichia coli K-12 (E. coli K-12)-stimulated SW480 cells in vitro. Methods The AGS cells and SW480 cells were pretreated with Ispaghul extract in concentrations, i. e. 3.5 and 7 μg/mL prior to infection with H. pylori and E. coli K-12. Results DNA fragmentation in AGS and SW480 cells treated with Ispaghul extract was not significant (2.3±0.8 %) compared with untreated cells (2.2±0.6 %). Ispaghul extract decreased the H. pylori-stimulated secretion of IL-1β by AGS cell (p<0.0001). This effect did not increase as the concentration of extract was increased. Ispaghul extract also decreased E. coli K-12-stimulated IL-1β secretion by SW480 cell (p<0.0001). This effect increased as the concentration of extracts was increased. Conclusions Ispaghul extract had an effect on stimulated secretion of IL-1β by the AGS and SW480 cell. It decreased pro-inflammatory reaction from both cell lines stimulated by bacteria.

  17. In planta comparative transcriptomics of host-adapted strains of Ralstonia solanacearum

    PubMed Central

    Ailloud, Florent; Lowe, Tiffany M.; Robène, Isabelle; Cruveiller, Stéphane; Allen, Caitilyn

    2016-01-01

    Background. Ralstonia solanacearum is an economically important plant pathogen with an unusually large host range. The Moko (banana) and NPB (not pathogenic to banana) strain groups are closely related but are adapted to distinct hosts. Previous comparative genomics studies uncovered very few differences that could account for the host range difference between these pathotypes. To better understand the basis of this host specificity, we used RNAseq to profile the transcriptomes of an R. solanacearum Moko strain and an NPB strain under in vitro and in planta conditions. Results. RNAs were sequenced from bacteria grown in rich and minimal media, and from bacteria extracted from mid-stage infected tomato, banana and melon plants. We computed differential expression between each pair of conditions to identify constitutive and host-specific gene expression differences between Moko and NPB. We found that type III secreted effectors were globally up-regulated upon plant cell contact in the NPB strain compared with the Moko strain. Genes encoding siderophore biosynthesis and nitrogen assimilation genes were highly up-regulated in the NPB strain during melon pathogenesis, while denitrification genes were up-regulated in the Moko strain during banana pathogenesis. The relatively lower expression of oxidases and the denitrification pathway during banana pathogenesis suggests that R. solanacearum experiences higher oxygen levels in banana pseudostems than in tomato or melon xylem. Conclusions. This study provides the first report of differential gene expression associated with host range variation. Despite minimal genomic divergence, the pathogenesis of Moko and NPB strains is characterized by striking differences in expression of virulence- and metabolism-related genes. PMID:26788428

  18. Abscisic acid metabolizing rhizobacteria decrease ABA concentrations in planta and alter plant growth.

    PubMed

    Belimov, Andrey A; Dodd, Ian C; Safronova, Vera I; Dumova, Valentina A; Shaposhnikov, Alexander I; Ladatko, Alexander G; Davies, William J

    2014-01-01

    Although endogenous phytohormones such as abscisic acid (ABA) regulate root growth, and many rhizobacteria can modulate root phytohormone status, hitherto there have been no reports of rhizobacteria mediating root ABA concentrations and growth by metabolising ABA. Using a selective ABA-supplemented medium, two bacterial strains were isolated from the rhizosphere of rice (Oryza sativa) seedlings grown in sod-podzolic soil and assigned to Rhodococcus sp. P1Y and Novosphingobium sp. P6W using partial 16S rRNA gene sequencing and phenotypic patterns by the GEN III MicroPlate test. Although strain P6W had more rapid growth in ABA-supplemented media than strain P1Y, both could utilize ABA as a sole carbon source in batch culture. When rice seeds were germinated on filter paper in association with bacteria, root ABA concentration was not affected, but shoot ABA concentration of inoculated plants decreased by 14% (strain P6W) and 22% (strain P1Y). When tomato (Solanum lycopersicum) genotypes differing in ABA biosynthesis (ABA deficient mutants flacca - flc, and notabilis - not and the wild-type cv. Ailsa Craig, WT) were grown in gnotobiotic cultures on nutrient solution agar, rhizobacterial inoculation decreased root and/or leaf ABA concentrations, depending on plant and bacteria genotypes. Strain P6W inhibited primary root elongation of all genotypes, but increased leaf biomass of WT plants. In WT plants treated with silver ions that inhibit ethylene perception, both ABA-metabolising strains significantly decreased root ABA concentration, and strain P6W decreased leaf ABA concentration. Since these changes in ABA status also occurred in plants that were not treated with silver, it suggests that ethylene was probably not involved in regulating bacteria-mediated changes in ABA concentration. Correlations between plant growth and ABA concentrations in planta suggest that ABA-metabolising rhizobacteria may stimulate growth via an ABA-dependent mechanism.

  19. Label-free chemically specific imaging in planta with stimulated Raman scattering microscopy.

    PubMed

    Mansfield, Jessica C; Littlejohn, George R; Seymour, Mark P; Lind, Rob J; Perfect, Sarah; Moger, Julian

    2013-05-21

    The growing world population puts ever-increasing demands on the agricultural and agrochemical industries to increase agricultural yields. This can only be achieved by investing in fundamental plant and agrochemical research and in the development of improved analytical tools to support research in these areas. There is currently a lack of analytical tools that provide noninvasive structural and chemical analysis of plant tissues at the cellular scale. Imaging techniques such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy provide label-free chemically specific image contrast based on vibrational spectroscopy. Over the past decade, these techniques have been shown to offer clear advantages for a vast range of biomedical research applications. The intrinsic vibrational contrast provides label-free quantitative functional analysis, it does not suffer from photobleaching, and it allows near real-time imaging in 3D with submicrometer spatial resolution. However, due to the susceptibility of current detection schemes to optical absorption and fluorescence from pigments (such as chlorophyll), the plant science and agrochemical research communities have not been able to benefit from these techniques and their application in plant research has remained virtually unexplored. In this paper, we explore the effect of chlorophyll fluorescence and absorption in CARS and SRS microscopy. We show that with the latter it is possible to use phase-sensitive detection to separate the vibrational signal from the (electronic) absorption processes. Finally, we demonstrate the potential of SRS for a range of in planta applications by presenting in situ chemical analysis of plant cell wall components, epicuticular waxes, and the deposition of agrochemical formulations onto the leaf surface.

  20. Floral benzenoid carboxyl methyltransferases: From in vitro to in planta function

    PubMed Central

    Effmert, Uta; Saschenbrecker, Sandra; Ross, Jeannine; Negre, Florence; Fraser, Chris M.; Noel, Joseph P.; Dudareva, Natalia; Piechulla, Birgit

    2010-01-01

    Benzenoid carboxyl methyltransferases synthesize methyl esters (e.g., methyl benzoate and methyl salicylate), which are constituents of aromas and scents of many plant species and play important roles in plant communication with the surrounding environment. Within the past five years, eleven such carboxyl methyltransferases were isolated and most of them were comprehensively investigated at the biochemical, molecular and structural level. Two types of enzymes can be distinguished according to their substrate preferences: the SAMT-type enzymes isolated from Clarkia breweri, Stephanotis floribunda, Antirrhinum majus, Hoya carnosa, and Petunia hybrida, which have a higher catalytic efficiency and preference for salicylic acid, while BAMT-type enzymes from A. majus, Arabidopsis thaliana, Arabidopsis lyrata, and Nicotiana suaveolens prefer benzoic acid. The elucidation of C. breweri SAMT’s three-dimensional structure allowed a detailed modelling of the active sites of the carboxyl methyltransferases and revealed that the SAM binding pocket is highly conserved among these enzymes while the methyl acceptor binding site exhibits some variability, allowing a classification into SAMT-type and BAMT-type enzymes. The analysis of expression patterns coupled with biochemical characterization showed that these carboxyl methyltransferases are involved either in floral scent biosynthesis or in plant defense responses. While the latter can be induced by biotic or abiotic stress, the genes responsible for floral scent synthesis exhibit developmental and rhythmic expression pattern. The nature of the product and efficiency of its formation in planta depend on the availability of substrates, the catalytic efficiency of the enzyme toward benzoic acid and/or salicylic acid, and the transcriptional, translational, and post-translational regulation at the enzyme level. The biochemical properties of benzenoid carboxyl methyltransferases suggest that the genes involved in plant defenses

  1. A rapid assay to quantify the cleavage efficiency of custom-designed nucleases in planta.

    PubMed

    Johnson, Ross A; Gurevich, Vyacheslav; Levy, Avraham A

    2013-06-01

    Custom-designed nucleases are a promising technology for genome editing through the catalysis of double-strand DNA breaks within target loci and subsequent repair by the host cell, which can result in targeted mutagenesis or gene replacement. Implementing this new technology requires a rapid means to determine the cleavage efficiency of these custom-designed proteins in planta. Here we present such an assay that is based on cleavage-dependent luciferase gene correction as part of a transient dual-luciferase(®) reporter (Promega) expression system. This assay consists of co-infiltrating Nicotiana benthamiana leaves with two Agrobacterium tumefaciens strains: one contains the target sequence embedded within a luciferase reporter gene and the second strain contains the custom-designed nuclease gene(s). We compared repair following site-specific nuclease digestion through non-homologous DNA end-joining, as opposed to single strand DNA annealing, as a means to restore an out-of-frame luciferase gene cleavage-reporter construct. We show, using luminometer measurements and bioluminescence imaging, that the assay for non-homologous end-joining is sensitive, quantitative, reproducible and rapid in estimating custom-designed nucleases' cleavage efficiency. We detected cleavage by two out of three transcription activator-like effector nucleases that we custom-designed for targets in the Arabidopsis CRUCIFERIN3 gene, and we compared with the well-established 'QQR' zinc-finger nuclease. The assay we report requires only standard equipment and basic plant molecular biology techniques, and it can be carried out within a few days. Different types of custom-designed nucleases can be preliminarily tested in our assay system before their downstream application in plant genome editing.

  2. Nodularin, a cyanobacterial toxin, is synthesized in planta by symbiotic Nostoc sp.

    PubMed

    Gehringer, Michelle M; Adler, Lewis; Roberts, Alexandra A; Moffitt, Michelle C; Mihali, Troco K; Mills, Toby J T; Fieker, Claus; Neilan, Brett A

    2012-10-01

    The nitrogen-fixing bacterium, Nostoc, is a commonly occurring cyanobacterium often found in symbiotic associations. We investigated the potential of cycad cyanobacterial endosymbionts to synthesize microcystin/nodularin. Endosymbiont DNA was screened for the aminotransferase domain of the toxin biosynthesis gene clusters. Five endosymbionts carrying the gene were screened for bioactivity. Extracts of two isolates inhibited protein phosphatase 2A and were further analyzed using electrospray ionization mass spectrometry (ESI-MS)/MS. Nostoc sp. 'Macrozamia riedlei 65.1' and Nostoc sp. 'Macrozamia serpentina 73.1' both contained nodularin. High performance liquid chromatography (HPLC) HESI-MS/MS analysis confirmed the presence of nodularin at 9.55±2.4 ng μg-1 chlorophyll a in Nostoc sp. 'Macrozamia riedlei 65.1' and 12.5±8.4 ng μg-1 Chl a in Nostoc sp. 'Macrozamia serpentina 73.1' extracts. Further scans indicated the presence of the rare isoform [L-Har(2)] nodularin, which contains L-homoarginine instead of L-arginine. Nodularin was also present at 1.34±0.74 ng ml(-1) (approximately 3 pmol per g plant ww) in the methanol root extracts of M. riedlei MZ65, while the presence of [L-Har(2)] nodularin in the roots of M. serpentina MZ73 was suggested by HPLC HESI-MS/MS analysis. The ndaA-B and ndaF genomic regions were sequenced to confirm the presence of the hybrid polyketide/non-ribosomal gene cluster. A seven amino-acid insertion into the NdaA-C1 domain of N. spumigena NSOR10 protein was observed in all endosymbiont-derived sequences, suggesting the transfer of the nda cluster from N. spumigena to terrestrial Nostoc species. This study demonstrates the synthesis of nodularin and [L-Har(2)] nodularin in a non-Nodularia species and the production of cyanobacterial hepatotoxin by a symbiont in planta.

  3. In planta expression of a mature Der p 1 allergen isolated from an Italian strain of Dermatophagoides pteronyssinus.

    PubMed

    Marconi, Gianpiero; Albertini, Emidio; Mari, Adriano; Palazzo, Paola; Porceddu, Andrea; Raggi, Lorenzo; Bolis, Luigi; Lancioni, Hovirag; Palomba, Antonella; Lucentini, Livia; Lanfaloni, Luisa; Marcucci, Francesco; Falcinelli, Mario; Panara, Fausto

    2012-06-01

    European (Dermatophagoides pteronyssinus) and American (Dermatophagoides farinae) house dust mite species are considered the most common causes of asthma and allergic symptoms worldwide. Der p 1 protein, one of the main allergens of D. pteronyssinus, is found in high concentration in mites faecal pellets, which can became easily airborne and, when inhaled, can cause perennial rhinitis and bronchial asthma. Here we report the isolation of the Der p 1 gene from an Italian strain of D. pteronyssinus and the PVX-mediated expression of its mature form (I-rDer p 1) in Nicotiana benthamiana plants. Human sera from characterized allergic patients were used for IgE binding inhibition assays to test the immunological reactivity of I-rDer p 1 produced in N. benthamiana plants. The binding properties of in planta produced I-rDer p 1 versus the IgE of patients sera were comparable to those obtained on Der p 1 preparation immobilized on a microarray. In this paper we provide a proof of concept for the production of an immunologically active form of Der p 1 using a plant viral vector. These results pave the way for the development of diagnostic allergy tests based on in planta produced allergens.

  4. Work function analysis of vegetarian entrée production.

    PubMed

    Maloney, S; Zolber, K; Burke, K; Connell, B; Shavlik, G

    1986-02-01

    Data on labor time for food production can be used as an effective management tool. It is essential for foodservice managers to know how labor time is being used (1). A continuous time study was conducted to determine total labor time for the production of eight vegetarian entrées in a hospital foodservice system. Two work areas were observed: the ingredient assembly area and the cooks' production area. Times were recorded by work function to identify how labor time was distributed. Results showed (a) observed frequency for each work function, (b) time expended in seconds per portion for each work function, (c) percentage distribution of labor time by work function, (d) total time for each employee involved in entrée production, and (e) percentage of total time in which each employee was involved in the production of each entrée. Total labor time varied by type of entrée, ranging from 39.97 to 19.33 seconds per portion. Entrées with the highest labor time required the largest amount of hand labor. A one-way analysis of variance indicated significant differences in mean labor time among the eight vegetarian entrées for direct labor time (p = .0009), and total labor time (p = .0018). No significant differences were found among entrées for indirect labor or delay time.

  5. A single base pair in the right terminal domain of Tomato planta macho viroid is a virulence determinant factor on tomato

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Tomato planta macho viroid (TPMVd), including isolates previously designated as Mexican papita viroid (MPVd), causes serious disease on tomatoes in North America. Two predominant variants, sharing 93.8% sequence identity, incited distinct severe (MPVd-S) or mild (MPVd-M) symptoms on tomato. To ide...

  6. Plastid-localized amino acid biosynthetic pathways of Plantae are predominantly composed of non-cyanobacterial enzymes

    PubMed Central

    Reyes-Prieto, Adrian; Moustafa, Ahmed

    2012-01-01

    Studies of photosynthetic eukaryotes have revealed that the evolution of plastids from cyanobacteria involved the recruitment of non-cyanobacterial proteins. Our phylogenetic survey of >100 Arabidopsis nuclear-encoded plastid enzymes involved in amino acid biosynthesis identified only 21 unambiguous cyanobacterial-derived proteins. Some of the several non-cyanobacterial plastid enzymes have a shared phylogenetic origin in the three Plantae lineages. We hypothesize that during the evolution of plastids some enzymes encoded in the host nuclear genome were mistargeted into the plastid. Then, the activity of those foreign enzymes was sustained by both the plastid metabolites and interactions with the native cyanobacterial enzymes. Some of the novel enzymatic activities were favored by selective compartmentation of additional complementary enzymes. The mosaic phylogenetic composition of the plastid amino acid biosynthetic pathways and the reduced number of plastid-encoded proteins of non-cyanobacterial origin suggest that enzyme recruitment underlies the recompartmentation of metabolic routes during the evolution of plastids. PMID:23233874

  7. Bioimaging techniques for subcellular localization of plant hemoglobins and measurement of hemoglobin-dependent nitric oxide scavenging in planta.

    PubMed

    Hebelstrup, Kim H; Østergaard-Jensen, Erik; Hill, Robert D

    2008-01-01

    Plant hemoglobins are ubiquitous in all plant families. They are expressed at low levels in specific tissues. Several studies have established that plant hemoglobins are scavengers of nitric oxide (NO) and that varying the endogenous level of hemoglobin in plant cells negatively modulates bioactivity of NO generated under hypoxic conditions or during cellular signaling. Earlier methods for determination of hemoglobin-dependent scavenging in planta were based on measuring activity in whole plants or organs. Plant hemoglobins do not contain specific organelle localization signals; however, earlier reports on plant hemoglobin have demonstrated either cytosolic or nuclear localization, depending on the method or cell type investigated. We have developed two bioimaging techniques: one for visualization of hemoglobin-catalyzed scavenging of NO in specific cells and another for visualization of subcellular localization of green fluorescent protein-tagged plant hemoglobins in transformed Arabidopsis thaliana plants.

  8. Homoserine and asparagine are host signals that trigger in planta expression of a pathogenesis gene in Nectria haematococca

    PubMed Central

    Yang, Zhennai; Rogers, Linda M.; Song, Yuanda; Guo, Wenjin; Kolattukudy, P. E.

    2005-01-01

    Some pathogenesis-related genes are expressed in fungi only when the pathogen is in the host, but the host signals that trigger these gene expressions have not been identified. Virulent Nectria haematococca infects pea plants and requires either pelA, which is induced by pectin, or pelD, which is induced only in planta. However, the host signal(s) that trigger pelD expression was unknown. Here we report the isolation of the host signals and identify homoserine and asparagine, two free amino acids found in uniquely high levels in pea seedlings, as the pelD-inducing signals. N. haematococca has evolved a mechanism to sense the host tissue environment by using the high levels of two free amino acids in this plant, thereby triggering the expression of pelD to assist the pathogenic process. PMID:15753300

  9. Phytohormonal networks promote differentiation of fiber initials on pre-anthesis cotton ovules grown in vitro and in planta.

    PubMed

    Kim, Hee Jin; Hinchliffe, Doug J; Triplett, Barbara A; Chen, Z Jeffrey; Stelly, David M; Yeater, Kathleen M; Moon, Hong S; Gilbert, Matthew K; Thyssen, Gregory N; Turley, Rickie B; Fang, David D

    2015-01-01

    The number of cotton (Gossypium sp.) ovule epidermal cells differentiating into fiber initials is an important factor affecting cotton yield and fiber quality. Despite extensive efforts in determining the molecular mechanisms regulating fiber initial differentiation, only a few genes responsible for fiber initial differentiation have been discovered. To identify putative genes directly involved in the fiber initiation process, we used a cotton ovule culture technique that controls the timing of fiber initial differentiation by exogenous phytohormone application in combination with comparative expression analyses between wild type and three fiberless mutants. The addition of exogenous auxin and gibberellins to pre-anthesis wild type ovules that did not have visible fiber initials increased the expression of genes affecting auxin, ethylene, ABA and jasmonic acid signaling pathways within 1 h after treatment. Most transcripts expressed differentially by the phytohormone treatment in vitro were also differentially expressed in the ovules of wild type and fiberless mutants that were grown in planta. In addition to MYB25-like, a gene that was previously shown to be associated with the differentiation of fiber initials, several other differentially expressed genes, including auxin/indole-3-acetic acid (AUX/IAA) involved in auxin signaling, ACC oxidase involved in ethylene biosynthesis, and abscisic acid (ABA) 8'-hydroxylase an enzyme that controls the rate of ABA catabolism, were co-regulated in the pre-anthesis ovules of both wild type and fiberless mutants. These results support the hypothesis that phytohormonal signaling networks regulate the temporal expression of genes responsible for differentiation of cotton fiber initials in vitro and in planta.

  10. In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance.

    PubMed

    Pandey, Sonika; Patel, Manish Kumar; Mishra, Avinash; Jha, Bhavanath

    2016-01-01

    Cumin is an annual, herbaceous, medicinal, aromatic, spice glycophyte that contains diverse applications as a food and flavoring additive, and therapeutic agents. An efficient, less time consuming, Agrobacterium-mediated, a tissue culture-independent in planta genetic transformation method was established for the first time using cumin seeds. The SbNHX1 gene, cloned from an extreme halophyte Salicornia brachiata was transformed in cumin using optimized in planta transformation method. The SbNHX1 gene encodes a vacuolar Na+/H+ antiporter and is involved in the compartmentalization of excess Na+ ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII) specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. Transgenic lines L3 and L13 showed high expression of the SbNHX1 gene compared to L6 whereas moderate expression was detected in L5 and L10 transgenic lines. Transgenic lines (L3, L5, L10 and L13), overexpressing the SbNHX1 gene, showed higher photosynthetic pigments (chlorophyll a, b and carotenoid), and lower electrolytic leakage, lipid peroxidation (MDA content) and proline content as compared to wild type plants under salinity stress. Though transgenic lines were also affected by salinity stress but performed better compared to WT plants. The ectopic expression of the SbNHX1 gene confirmed enhanced salinity stress tolerance in cumin as compared to wild type plants under stress condition. The present study is the first report of engineering salt tolerance in cumin, so far and the plant may be utilized for the cultivation in saline areas.

  11. Development of fiber optic spectroscopy for in-vitro and in-planta detection of fluorescent proteins

    NASA Astrophysics Data System (ADS)

    Liew, Oi Wah; Chen, Jun-Wei; Asundi, Anand K.

    2001-10-01

    The objective of this project is to apply photonics technology to bio-safety management of genetically modified (GM) plants. The conventional method for screening GM plants is through selection using antibiotic resistance markers. There is public concern with such approaches and these are associated with food safety issues, escape of antibiotic resistance genes to pathogenic microorganisms and interference with antibiotic therapy. Thus, the strategy taken in this project is to replace antibiotic resistance markers with fluorescent protein markers that allow for rapid and non-invasive optical screening of genetically modified plants. In this paper, fibre optic spectroscopy was developed to detect and quantify recombinant green (EGFP) and red (DsRED) fluorescent proteins in vitro and in planta. In vitro detection was first carried out to optimize the sensitivity of the optical system. The bacterial expression vectors carrying the coding regions of EGFP and DsRED were introduced into Escherichia coli host cells and fluorescent proteins were produced following induction with IPTG. Soluble EGFP and DsRED proteins were isolated from lysed bacterial cells and serially diluted for quantitative analysis by fibre optic spectroscopy using different light sources, namely, blue LED (475 nm), tungsten halogen (350 - 1000 nm) and double frequency Nd:YAG green laser (532 nm). Fluorescence near the expected emission wavelengths could be detected up to 320X dilution for EGFP and DsRED with blue LED and 532 nm green laser, respectively, as the excitation source. Tungsten halogen was found to be unsuitable for excitation of both EGFP and DsRED. EGFP was successfully purified by size separation under non-denaturing electrophoretic conditions and quantified. The minimum concentration of EGFP detectable with blue LED excitation was 5 mg/ml. To determine the capability of spectroscopy detection in planta, transgenic potato hairy roots and whole modified plant lines expressing the

  12. In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance

    PubMed Central

    Pandey, Sonika; Patel, Manish Kumar; Jha, Bhavanath

    2016-01-01

    Cumin is an annual, herbaceous, medicinal, aromatic, spice glycophyte that contains diverse applications as a food and flavoring additive, and therapeutic agents. An efficient, less time consuming, Agrobacterium-mediated, a tissue culture-independent in planta genetic transformation method was established for the first time using cumin seeds. The SbNHX1 gene, cloned from an extreme halophyte Salicornia brachiata was transformed in cumin using optimized in planta transformation method. The SbNHX1 gene encodes a vacuolar Na+/H+ antiporter and is involved in the compartmentalization of excess Na+ ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII) specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. Transgenic lines L3 and L13 showed high expression of the SbNHX1 gene compared to L6 whereas moderate expression was detected in L5 and L10 transgenic lines. Transgenic lines (L3, L5, L10 and L13), overexpressing the SbNHX1 gene, showed higher photosynthetic pigments (chlorophyll a, b and carotenoid), and lower electrolytic leakage, lipid peroxidation (MDA content) and proline content as compared to wild type plants under salinity stress. Though transgenic lines were also affected by salinity stress but performed better compared to WT plants. The ectopic expression of the SbNHX1 gene confirmed enhanced salinity stress tolerance in cumin as compared to wild type plants under stress condition. The present study is the first report of engineering salt tolerance in cumin, so far and the plant may be utilized for the cultivation in saline areas. PMID:27411057

  13. The in planta transcriptome of Ralstonia solanacearum: conserved physiological and virulence strategies during bacterial wilt of tomato.

    PubMed

    Jacobs, Jonathan M; Babujee, Lavanya; Meng, Fanhong; Milling, Annett; Allen, Caitilyn

    2012-01-01

    Plant xylem fluid is considered a nutrient-poor environment, but the bacterial wilt pathogen Ralstonia solanacearum is well adapted to it, growing to 10(8) to 10(9) CFU/g tomato stem. To better understand how R. solanacearum succeeds in this habitat, we analyzed the transcriptomes of two phylogenetically distinct R. solanacearum strains that both wilt tomato, strains UW551 (phylotype II) and GMI1000 (phylotype I). We profiled bacterial gene expression at ~6 × 10(8) CFU/ml in culture or in plant xylem during early tomato bacterial wilt pathogenesis. Despite phylogenetic differences, these two strains expressed their 3,477 common orthologous genes in generally similar patterns, with about 12% of their transcriptomes significantly altered in planta versus in rich medium. Several primary metabolic pathways were highly expressed during pathogenesis. These pathways included sucrose uptake and catabolism, and components of these pathways were encoded by genes in the scrABY cluster. A UW551 scrA mutant was significantly reduced in virulence on resistant and susceptible tomato as well as on potato and the epidemiologically important weed host Solanum dulcamara. Functional scrA contributed to pathogen competitive fitness during colonization of tomato xylem, which contained ~300 µM sucrose. scrA expression was induced by sucrose, but to a much greater degree by growth in planta. Unexpectedly, 45% of the genes directly regulated by HrpB, the transcriptional activator of the type 3 secretion system (T3SS), were upregulated in planta at high cell densities. This result modifies a regulatory model based on bacterial behavior in culture, where this key virulence factor is repressed at high cell densities. The active transcription of these genes in wilting plants suggests that T3SS has a biological role throughout the disease cycle. IMPORTANCE Ralstonia solanacearum is a widespread plant pathogen that causes bacterial wilt disease. It inflicts serious crop losses on tropical

  14. Relación Entre el Tamaño Condilar y la Asimetría Facial Transversal en Individuos con Hiperplasia Condilar

    PubMed Central

    Olate, Sergio; Cantín, Mario; Alister, Juan Pablo; Uribe, Francisca; Navarro, Pablo; Olate, Gabriela; de Moraes, Márcio

    2016-01-01

    La asimetría facial es una patología de compromiso funcional y estético que puede estar generado por hiperplasia condilar; el objetivo de esta investigación es determinar la influencia del tamaño condilar en la asimetría facial. Doce sujetos fueron estudiados mediante el análisis de tomografía computadorizada cone beam; todos los pacientes presentaron cintigrama óseo que determino la presencia de hiperplasia condilar activa; el análisis se realizo en un software del sistema de captura modelo Pax Zenith, marca Vatech (Korea 2011), utilizando 90 kV y 120 mA; se obtuvo mediciones de la distancia antero-posterior, superior-inferior y medio-lateral de la cabeza del cóndilo, relacionándose con la posición del cóndilo con la línea mediana facial y la posición de puntos faciales entre incisivos centrales superior e inferior así como con el mentón. Los resultados mostraron un promedio de desvío de mentón de 6,5 mm considerando un cóndilo hiperplásico con un tamaño de 2,7 mm mas que los cóndilos normales. El desvío de incisivo central inferior determinó que por cada 1 mm de desvío dentario existe 2,2 mm de desvío de mentón. El cóndilo hiperplásico fue de mayor tamaño y se posiciono casi 2 mm mas hacia lateral que los cóndilos no hiperplásicos. Es posible concluir que el cóndilo hiperplásico presenta clara influencia en la asimetría facial transversa y es posible estimar una relación de tamaño condilar con grado de asimetría facial. PMID:28066127

  15. The Issue of Motivating Entre(Intra)Preneurial Behavior.

    ERIC Educational Resources Information Center

    Winslow, Erik K.

    1990-01-01

    Six principles of motivating entre(intra)preneurial behavior are considered including the climate must allow the expression of such activity; motivation is broadly distributed in the general population; behavior is a function of its consequences; and motivating environments have an aura of excitement and experimentation. (DB)

  16. Construction of EGFP-labeling system for visualizing the infection process of Xanthomonas axonopodis pv. citri in planta.

    PubMed

    Liu, Li-Ping; Deng, Zi-Niu; Qu, Jin-Wang; Yan, Jia-Wen; Catara, Vittoria; Li, Da-Zhi; Long, Gui-You; Li, Na

    2012-09-01

    Xanthomonas axonopodis pv. citri (Xac) is the causal agent of citrus bacterial canker, an economically important disease to world citrus industry. To monitor the infection process of Xac in different citrus plants, the enhanced green florescent protein (EGFP) visualizing system was constructed to visualize the propagation and localization in planta. First, the wild-type Xac was isolated from the diseased leaves of susceptible 'Bingtang' sweet orange, and then the isolated Xac was labeled with EGFP by triparental mating. After PCR identification, the growth kinetics and pathogenicity of the transformants were analyzed in comparison with the wild-type Xac. The EGFP-labeled bacteria were inoculated by spraying on the surface and infiltration in the mesophyll of 'Bingtang' sweet orange leaves. The bacterial cell multiplication and diffusion processes were observed directly under confocal laser scanning microscope at different intervals after inoculation. The results indicated that the EGFP-labeled Xac releasing clear green fluorescence light under fluorescent microscope showed the infection process and had the same pathogenicity as the wild type to citrus. Consequently, the labeled Xac demonstrated the ability as an efficient tool to monitor the pathogen infection.

  17. In Planta Single-Molecule Pull-Down Reveals Tetrameric Stoichiometry of HD-ZIPIII:LITTLE ZIPPER Complexes[OPEN

    PubMed Central

    Husbands, Aman Y.; Aggarwal, Vasudha; Ha, Taekjip; Timmermans, Marja C.P.

    2016-01-01

    Deciphering complex biological processes markedly benefits from approaches that directly assess the underlying biomolecular interactions. Most commonly used approaches to monitor protein-protein interactions typically provide nonquantitative readouts that lack statistical power and do not yield information on the heterogeneity or stoichiometry of protein complexes. Single-molecule pull-down (SiMPull) uses single-molecule fluorescence detection to mitigate these disadvantages and can quantitatively interrogate interactions between proteins and other compounds, such as nucleic acids, small molecule ligands, and lipids. Here, we establish SiMPull in plants using the HOMEODOMAIN LEUCINE ZIPPER III (HD-ZIPIII) and LITTLE ZIPPER (ZPR) interaction as proof-of-principle. Colocalization analysis of fluorophore-tagged HD-ZIPIII and ZPR proteins provides strong statistical evidence of complex formation. In addition, we use SiMPull to directly quantify YFP and mCherry maturation probabilities, showing these differ substantially from values obtained in mammalian systems. Leveraging these probabilities, in conjunction with fluorophore photobleaching assays on over 2000 individual complexes, we determined HD-ZIPIII:ZPR stoichiometry. Intriguingly, these complexes appear as heterotetramers, comprising two HD-ZIPIII and two ZPR molecules, rather than heterodimers as described in the current model. This surprising result raises new questions about the regulation of these key developmental factors and is illustrative of the unique contribution SiMPull is poised to make to in planta protein interaction studies. PMID:27385814

  18. In Planta Single-Molecule Pull-Down Reveals Tetrameric Stoichiometry of HD-ZIPIII:LITTLE ZIPPER Complexes.

    PubMed

    Husbands, Aman Y; Aggarwal, Vasudha; Ha, Taekjip; Timmermans, Marja C P

    2016-08-01

    Deciphering complex biological processes markedly benefits from approaches that directly assess the underlying biomolecular interactions. Most commonly used approaches to monitor protein-protein interactions typically provide nonquantitative readouts that lack statistical power and do not yield information on the heterogeneity or stoichiometry of protein complexes. Single-molecule pull-down (SiMPull) uses single-molecule fluorescence detection to mitigate these disadvantages and can quantitatively interrogate interactions between proteins and other compounds, such as nucleic acids, small molecule ligands, and lipids. Here, we establish SiMPull in plants using the HOMEODOMAIN LEUCINE ZIPPER III (HD-ZIPIII) and LITTLE ZIPPER (ZPR) interaction as proof-of-principle. Colocalization analysis of fluorophore-tagged HD-ZIPIII and ZPR proteins provides strong statistical evidence of complex formation. In addition, we use SiMPull to directly quantify YFP and mCherry maturation probabilities, showing these differ substantially from values obtained in mammalian systems. Leveraging these probabilities, in conjunction with fluorophore photobleaching assays on over 2000 individual complexes, we determined HD-ZIPIII:ZPR stoichiometry. Intriguingly, these complexes appear as heterotetramers, comprising two HD-ZIPIII and two ZPR molecules, rather than heterodimers as described in the current model. This surprising result raises new questions about the regulation of these key developmental factors and is illustrative of the unique contribution SiMPull is poised to make to in planta protein interaction studies.

  19. Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice.

    PubMed

    Yang, Haibing; Wei, Hui; Ma, Guojie; Antunes, Mauricio S; Vogt, Stefan; Cox, Joseph; Zhang, Xiao; Liu, Xiping; Bu, Lintao; Gleber, S Charlotte; Carpita, Nicholas C; Makowski, Lee; Himmel, Michael E; Tucker, Melvin P; McCann, Maureen C; Murphy, Angus S; Peer, Wendy A

    2016-10-01

    Conversion of nongrain biomass into liquid fuel is a sustainable approach to energy demands as global population increases. Previously, we showed that iron can act as a catalyst to enhance the degradation of lignocellulosic biomass for biofuel production. However, direct addition of iron catalysts to biomass pretreatment is diffusion-limited, would increase the cost and complexity of biorefinery unit operations and may have deleterious environmental impacts. Here, we show a new strategy for in planta accumulation of iron throughout the volume of the cell wall where iron acts as a catalyst in the deconstruction of lignocellulosic biomass. We engineered CBM-IBP fusion polypeptides composed of a carbohydrate-binding module family 11 (CBM11) and an iron-binding peptide (IBP) for secretion into Arabidopsis and rice cell walls. CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls. Further, CBM-IBP rice shows a 35% increase in seed iron concentration and a 40% increase in seed yield in greenhouse experiments. CBM-IBP rice potentially could be used to address iron deficiency, the most common and widespread nutritional disorder according to the World Health Organization.

  20. Overexpression of the rice carotenoid cleavage dioxygenase 1 gene in Golden Rice endosperm suggests apocarotenoids as substrates in planta.

    PubMed

    Ilg, Andrea; Yu, Qiuju; Schaub, Patrick; Beyer, Peter; Al-Babili, Salim

    2010-08-01

    Carotenoids are converted by carotenoid cleavage dioxygenases that catalyze oxidative cleavage reactions leading to apocarotenoids. However, apocarotenoids can also be further truncated by some members of this enzyme family. The plant carotenoid cleavage dioxygenase 1 (CCD1) subfamily is known to degrade both carotenoids and apocarotenoids in vitro, leading to different volatile compounds. In this study, we investigated the impact of the rice CCD1 (OsCCD1) on the pigmentation of Golden Rice 2 (GR2), a genetically modified rice variety accumulating carotenoids in the endosperm. For this purpose, the corresponding cDNA was introduced into the rice genome under the control of an endosperm-specific promoter in sense and anti-sense orientations. Despite high expression levels of OsCCD1 in sense plants, pigment analysis revealed carotenoid levels and patterns comparable to those of GR2, pleading against carotenoids as substrates in rice endosperm. In support, similar carotenoid contents were determined in anti-sense plants. To check whether OsCCD1 overexpressed in GR2 endosperm is active, in vitro assays were performed with apocarotenoid substrates. HPLC analysis confirmed the cleavage activity of introduced OsCCD1. Our data indicate that apocarotenoids rather than carotenoids are the substrates of OsCCD1 in planta.

  1. A rapid, highly efficient and economical method of Agrobacterium-mediated in planta transient transformation in living onion epidermis.

    PubMed

    Xu, Kedong; Huang, Xiaohui; Wu, Manman; Wang, Yan; Chang, Yunxia; Liu, Kun; Zhang, Ju; Zhang, Yi; Zhang, Fuli; Yi, Liming; Li, Tingting; Wang, Ruiyue; Tan, Guangxuan; Li, Chengwei

    2014-01-01

    Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of proteins. Onion epidermis, which has large, living and transparent cells in a monolayer, is suitable to visualize fluorescent fusion proteins. The often used transient transformation methods included particle bombardment, protoplast transfection and Agrobacterium-mediated transformation. Particle bombardment in onion epidermis was successfully established, however, it was expensive, biolistic equipment dependent and with low transformation efficiency. We developed a highly efficient in planta transient transformation method in onion epidermis by using a special agroinfiltration method, which could be fulfilled within 5 days from the pretreatment of onion bulb to the best time-point for analyzing gene expression. The transformation conditions were optimized to achieve 43.87% transformation efficiency in living onion epidermis. The developed method has advantages in cost, time-consuming, equipment dependency and transformation efficiency in contrast with those methods of particle bombardment in onion epidermal cells, protoplast transfection and Agrobacterium-mediated transient transformation in leaf epidermal cells of other plants. It will facilitate the analysis of protein subcellular localization on a large scale.

  2. A Rapid, Highly Efficient and Economical Method of Agrobacterium-Mediated In planta Transient Transformation in Living Onion Epidermis

    PubMed Central

    Xu, Kedong; Huang, Xiaohui; Wu, Manman; Wang, Yan; Chang, Yunxia; Liu, Kun; Zhang, Ju; Zhang, Yi; Zhang, Fuli; Yi, Liming; Li, Tingting; Wang, Ruiyue; Tan, Guangxuan; Li, Chengwei

    2014-01-01

    Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of proteins. Onion epidermis, which has large, living and transparent cells in a monolayer, is suitable to visualize fluorescent fusion proteins. The often used transient transformation methods included particle bombardment, protoplast transfection and Agrobacterium-mediated transformation. Particle bombardment in onion epidermis was successfully established, however, it was expensive, biolistic equipment dependent and with low transformation efficiency. We developed a highly efficient in planta transient transformation method in onion epidermis by using a special agroinfiltration method, which could be fulfilled within 5 days from the pretreatment of onion bulb to the best time-point for analyzing gene expression. The transformation conditions were optimized to achieve 43.87% transformation efficiency in living onion epidermis. The developed method has advantages in cost, time-consuming, equipment dependency and transformation efficiency in contrast with those methods of particle bombardment in onion epidermal cells, protoplast transfection and Agrobacterium-mediated transient transformation in leaf epidermal cells of other plants. It will facilitate the analysis of protein subcellular localization on a large scale. PMID:24416168

  3. Closed-reference metatranscriptomics enables in planta profiling of putative virulence activities in the grapevine trunk-disease complex.

    PubMed

    Morales-Cruz, Abraham; Allenbeck, Gabrielle; Figueroa-Balderas, Rosa; Ashworth, Vanessa E; Lawrence, Daniel P; Travadon, Renaud; Smith, Rhonda J; Baumgartner, Kendra; Rolshausen, Philippe E; Cantu, Dario

    2017-02-20

    Grapevines, like other perennial crops, are affected by so-called 'trunk diseases', which damage the trunk and other woody tissues. Mature grapevines typically contract more than one trunk disease and often multiple grapevine trunk pathogens (GTPs) are recovered from infected tissues. The co-existence of different GTP species in complex and dynamic microbial communities complicates the study of the molecular mechanisms underlying disease development especially under vineyard conditions. The objective of this study was to develop and optimize a community-level transcriptomics (i.e., metatranscriptomics) approach that can monitor simultaneously the virulence activities of multiple GTPs in planta. The availability of annotated genomes for the most relevant co-infecting GTPs in diseased grapevine wood provided the unprecedented opportunity to generate a multi-species reference for mapping and quantifying DNA and RNA sequencing reads. We first evaluated popular sequence read mappers using permutations of multiple simulated datasets. Alignment parameters of the selected mapper were optimized to increase the specificity and sensitivity for its application to metagenomics and metatranscriptomics analyses. Initial testing on grapevine wood experimentally inoculated with individual GTPs confirmed the validity of the method. Using naturally-infected field samples expressing a variety of trunk disease symptoms, we show that our approach provides quantitative assessments of species composition as well as genome-wide transcriptional profiling of potential virulence factors, namely cell wall degradation, secondary metabolism and nutrient uptake for all co-infecting GTPs. This article is protected by copyright. All rights reserved.

  4. In planta distribution of 'Candidatus Liberibacter asiaticus' as revealed by polymerase chain reaction (PCR) and real-time PCR.

    PubMed

    Tatineni, Satyanarayana; Sagaram, Uma Shankar; Gowda, Siddarame; Robertson, Cecile J; Dawson, William O; Iwanami, Toru; Wang, Nian

    2008-05-01

    Huanglongbing (HLB) is one of the most devastating diseases of citrus worldwide, and is caused by a phloem-limited fastidious prokaryotic alpha-proteobacterium that is yet to be cultured. In this study, a combination of traditional polymerase chain reaction (PCR) and real-time PCR targeting the putative DNA polymerase and 16S rDNA sequence of 'Candidatus Liberibacter asiaticus,' respectively, were used to examine the distribution and movement of the HLB pathogen in the infected citrus tree. We found that 'Ca. Liberibacter asiaticus' was distributed in bark tissue, leaf midrib, roots, and different floral and fruit parts, but not in endosperm and embryo, of infected citrus trees. Quantification analysis of the HLB bacterium indicated that it was distributed unevenly in planta and ranged from 14 to 137,031 cells/mug of total DNA in different tissues. A relatively high concentration of 'Ca. Liberibacter asiaticus' was observed in fruit peduncles. Our data from greenhouse-infected plants also indicated that 'Ca. Liberibacter asiaticus' was transmitted systemically from infection site to different parts of the plant. Understanding the distribution and movement of the HLB bacterium inside an individual citrus tree is critical for discerning its virulence mechanism and to develop management strategies for HLB.

  5. Functional characterisation of metal(loid) processes in planta through the integration of synchrotron techniques and plant molecular biology

    PubMed Central

    Donner, Erica; Punshon, Tracy; Guerinot, Mary Lou; Lombi, Enzo

    2013-01-01

    Functional characterisation of the genes regulating metal(loid) homeostasis in plants is a major focus of crop biofortification, phytoremediation, and food security research. This paper focuses on the potential for advancing plant metal(loid) research by combining molecular biology and synchrotron-based techniques. Recent advances in x-ray focussing optics and fluorescence detection have greatly improved the potential of synchrotron techniques for plant science research, allowing metal(loids) to be imaged in vivo in hydrated plant tissues at sub-micron resolution. Laterally resolved metal(loid) speciation can also be determined. By using molecular techniques to probe the location of gene expression and protein localisation and combining it with this synchrotron-derived data, functional information can be effectively and efficiently assigned to specific genes. This paper provides a review of the state of the art in this field, and provides examples as to how synchrotron-based methods can be combined with molecular techniques to facilitate functional characterisation of genes in planta. PMID:22200921

  6. Effective and specific in planta RNAi in cyst nematodes: expression interference of four parasitism genes reduces parasitic success.

    PubMed

    Sindhu, Anoop S; Maier, Tom R; Mitchum, Melissa G; Hussey, Richard S; Davis, Eric L; Baum, Thomas J

    2009-01-01

    Cyst nematodes are highly evolved sedentary plant endoparasites that use parasitism proteins injected through the stylet into host tissues to successfully parasitize plants. These secretory proteins likely are essential for parasitism as they are involved in a variety of parasitic events leading to the establishment of specialized feeding cells required by the nematode to obtain nourishment. With the advent of RNA interference (RNAi) technology and the demonstration of host-induced gene silencing in parasites, a new strategy to control pests and pathogens has become available, particularly in root-knot nematodes. Plant host-induced silencing of cyst nematode genes so far has had only limited success but similarly should disrupt the parasitic cycle and render the host plant resistant. Additional in planta RNAi data for cyst nematodes are being provided by targeting four parasitism genes through host-induced RNAi gene silencing in transgenic Arabidopsis thaliana, which is a host for the sugar beet cyst nematode Heterodera schachtii. Here it is reported that mRNA abundances of targeted nematode genes were specifically reduced in nematodes feeding on plants expressing corresponding RNAi constructs. Furthermore, this host-induced RNAi of all four nematode parasitism genes led to a reduction in the number of mature nematode females. Although no complete resistance was observed, the reduction of developing females ranged from 23% to 64% in different RNAi lines. These observations demonstrate the relevance of the targeted parasitism genes during the nematode life cycle and, potentially more importantly, suggest that a viable level of resistance in crop plants may be accomplished in the future using this technology against cyst nematodes.

  7. Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity.

    PubMed

    Cheng, Tao; Xu, Chao; Lei, Li; Li, Changhao; Zhang, Yu; Zhou, Shiliang

    2016-01-01

    The internal transcribed spacer (ITS) of nuclear ribosomal DNA is one of the most commonly used DNA markers in plant phylogenetic and DNA barcoding analyses, and it has been recommended as a core plant DNA barcode. Despite this popularity, the universality and specificity of PCR primers for the ITS region are not satisfactory, resulting in amplification and sequencing difficulties. By thoroughly surveying and analysing the 18S, 5.8S and 26S sequences of Plantae and Fungi from GenBank, we designed new universal and plant-specific PCR primers for amplifying the whole ITS region and a part of it (ITS1 or ITS2) of plants. In silico analyses of the new and the existing ITS primers based on these highly representative data sets indicated that (i) the newly designed universal primers are suitable for over 95% of plants in most groups; and (ii) the plant-specific primers are suitable for over 85% of plants in most groups without amplification of fungi. A total of 335 samples from 219 angiosperm families, 11 gymnosperm families, 24 fern and lycophyte families, 16 moss families and 17 fungus families were used to test the performances of these primers. In vitro PCR produced similar results to those from the in silico analyses. Our new primer pairs gave PCR improvements up to 30% compared with common-used ones. The new universal ITS primers will find wide application in both plant and fungal biology, and the new plant-specific ITS primers will, by eliminating PCR amplification of nonplant templates, significantly improve the quality of ITS sequence information collections in plant molecular systematics and DNA barcoding.

  8. Requirement of the galU Gene for Polysaccharide Production by and Pathogenicity and Growth In Planta of Xanthomonas citri subsp. citri▿

    PubMed Central

    Guo, Yinping; Sagaram, Uma Shankar; Kim, Jeong-soon; Wang, Nian

    2010-01-01

    Xanthomonas citri subsp. citri is the causal agent of citrus canker, which has a significant impact on citrus production. In this study, we characterized the galU gene of X. citri subsp. citri. Two galU mutants (F6 and D12) were identified in an X. citri subsp. citri EZ-Tn5 Tnp transposon library. Rescue cloning, sequence analysis, and Southern blot analysis indicated that both of these mutants had a single copy of the EZ-Tn5 transposon inserted in galU in the chromosome. Further study showed that galU was required for biosynthesis of extracellular polysaccharides (EPS; xanthan gum) and capsular polysaccharide (CPS) and biofilm formation. Mutation of galU resulted in a loss of pathogenicity for grapefruit. The loss of pathogenicity of a galU mutant resulted from its inability to grow in planta rather than from the effect on virulence genes. Quantitative reverse transcription-PCR assays indicated that mutation of galU did not impair the expression of key virulence genes, such as pthA of X. citri subsp. citri. Although D12 had a growth rate similar to that of the wild-type strain in nutrient broth, no D12 population became established in the intercellular spaces of citrus leaves. Coinoculation of a galU mutant with the wild-type strain did not promote growth of the galU mutant in planta. Defects in EPS and CPS production, pathogenicity, and growth in planta of the galU mutant were complemented to the wild-type level using plasmid pCGU2.1 containing an intact galU gene. These data indicate that the galU gene contributes to X. citri subsp. citri growth in intercellular spaces and is involved in EPS and CPS synthesis and biofilm formation. PMID:20118360

  9. Astronomy at School: Measurements of the Earth-Moon Distance. (Spanish Title: Astronomía en la Escuela: Medición de la Distancia Tierra-Luna.) Astronomia na Escola: Medida da Distância Terra-Lua

    NASA Astrophysics Data System (ADS)

    Paolantonio, Santiago; Pintado, Olga I.

    2006-12-01

    The aim of this project is to approach High School students to Science. We propose to measure the Earth-Moon distance using the parallax. The observation and measurements techniques and all the calculations needed are described in this paper. The results obtained in a test experience realized during 2003 are presented. This test shows that this is a feasible project and also shows which things needed to be improved. Se plantea la propuesta de un proyecto educativo dirigido al Nivel Medio de enseñanza, consistente en la determinación de la distancia Tierra - Luna por paralaje. Se describen las técnicas de observación, mediciones y cálculos. Se proporcionan a continuación los resultados obtenidos en la experiencia piloto realizada en el 2003, la que permitió comprobar la factibilidad de llevar a cabo la propuesta, así como perfeccionar los diferentes aspectos involucrados en la misma. Propomos neste trabalho um projeto educativo dirigido ao Ensino Médio que consiste na determinação da distância Terra-Lua pelo efeito da paralaxe. As técnicas de observação, medidas e cálculos auxiliares são descritos, e os resultados obtidos numa experiência-teste realizada no ano de 2003 apresentados. Este último teste permitiu comprovar a viabilidade de execução da proposta e aperfeiçoar diversos aspectos da mesma.

  10. Mobilization of horizontally acquired island 2 is induced in planta in the phytopathogen Pectobacterium atrosepticum SCRI1043 and involves the putative relaxase ECA0613 and quorum sensing.

    PubMed

    Vanga, Bhanupratap R; Ramakrishnan, Pavithra; Butler, Ruth C; Toth, Ian K; Ronson, Clive W; Jacobs, Jeanne M E; Pitman, Andrew R

    2015-11-01

    Integrative and conjugative elements (ICEs) contribute to the rapid evolution of bacterial pathogens via horizontal gene transfer of virulence determinants. ICEs have common mechanisms for transmission, yet the cues triggering this process under natural environmental or physiological conditions are largely unknown. In this study, mobilization of the putative ICE horizontally acquired island 2 (HAI2), present in the chromosome of the phytopathogen Pectobacterium atrosepticum SCRI1043, was examined during infection of the host plant potato. Under these conditions, mobilization of HAI2 increased markedly compared with in vitro cultures. In planta-induced mobilization of HAI2 was regulated by quorum sensing and involved the putative ICE-encoded relaxase ECA0613. Disruption of ECA0613 also reduced transcription of genes involved in production of coronafacic acid (Cfa), the major virulence factor harboured on HAI2, whereas their expression was unaffected in the quorum-sensing (expI) mutant. Thus, suppression of cfa gene expression was not regulated by the mobilization of the ICE per se, but was due directly to inactivation of the relaxase. The identification of genetic factors associated solely with in planta mobilization of an ICE demonstrates that this process is highly adapted to the natural environment of the bacterial host and can influence the expression of virulence determinants.

  11. RNA-Seq Revealed Differences in Transcriptomes between 3ADON and 15ADON Populations of Fusarium graminearum In Vitro and In Planta

    PubMed Central

    Puri, Krishna D.; Yan, Changhui; Leng, Yueqiang

    2016-01-01

    Fusarium graminearum is the major causal agent of Fusarium head blight (FHB) in barley and wheat in North America. The fungus not only causes yield loss of the crops but also produces harmful trichothecene mycotoxins [Deoxynivalenol (DON) and its derivatives-3-acetyldeoxynivalenol (3ADON) and 15-acetyldeoxynivalenol (15ADON), and nivalenol (NIV)] that contaminate grains. Previous studies showed a dramatic increase of 3ADON-producing isolates with higher aggressiveness and DON production than the 15ADON-producing isolates in North America. However, the genetic and molecular basis of differences between the two types of isolates is unclear. In this study, we compared transcriptomes of the 3ADON and 15ADON isolates in vitro (in culture media) and in planta (during infection on the susceptible wheat cultivar ‘Briggs’) using RNA-sequencing. The in vitro gene expression comparison identified 479 up-regulated and 801 down-regulated genes in the 3ADON isolates; the up-regulated genes were mainly involved in C-compound and carbohydrate metabolism (18.6%), polysaccharide metabolism (7.7%) or were of unknown functions (57.6%). The in planta gene expression analysis revealed that 185, 89, and 62 genes were up-regulated in the 3ADON population at 48, 96, and 144 hours after inoculation (HAI), respectively. The up-regulated genes were significantly enriched in functions for cellular import, C-compound and carbohydrate metabolism, allantoin and allantoate transport at 48 HAI, for detoxification and virulence at 96 HAI, and for metabolism of acetic acid derivatives, detoxification, and cellular import at 144 HAI. Comparative analyses of in planta versus in vitro gene expression further revealed 2,159, 1,981 and 2,095 genes up-regulated in the 3ADON isolates, and 2,415, 2,059 and 1,777 genes up-regulated in the 15ADON isolates at the three time points after inoculation. Collectively, our data provides a foundation for further understanding of molecular mechanisms involved in

  12. Use of attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy in direct, non-destructive, and rapid assessment of developmental cotton fibers grown in planta and in culture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cotton fibers are routinely harvested from cotton plants (in planta), and their end-use qualities depend on their development stages. Cotton fibers are also cultured at controlled laboratory environments, so that cotton researchers can investigate many aspects of experimental protocols in cotton bre...

  13. Forward genetic in planta screen for identification of plant-protective traits of Sphingomonas sp. strain Fr1 against Pseudomonas syringae DC3000.

    PubMed

    Vogel, Christine; Innerebner, Gerd; Zingg, Judith; Guder, Jan; Vorholt, Julia A

    2012-08-01

    Sphingomonas sp. strain Fr1 has recently been shown to protect Arabidopsis thaliana against the bacterial leaf pathogen Pseudomonas syringae DC3000. Here, we describe a forward genetic in planta screen to identify genes in Sphingomonas sp. Fr1 necessary for this effect. About 5,000 Sphingomonas sp. Fr1 mini-Tn5 mutants were assayed for a defect in plant protection against a luxCDABE-tagged P. syringae DC3000 derivative in a space-saving 24-well plate system. The bioluminescence of the pathogen was used as the indicator of pathogen proliferation and allowed for the identification of Sphingomonas sp. Fr1 mutants that had lost the ability to restrict pathogen growth before disease symptoms were visible. Potential candidates were validated using the same miniaturized experimental system. Of these mutants, 10 were confirmed as plant protection defective yet colonization competent. The mutants were subsequently evaluated in a previously described standard microbox system, and plants showed enhanced disease phenotypes after pathogen infection relative to those inoculated with the parental strain as a control. However, the disease severities were lower than those observed for control plants that were grown axenically prior to pathogen challenge, which suggests that several traits may contribute to plant protection. Transposon insertion sites of validated mutants with defects in plant protection were determined and mapped to 7 distinct genomic regions. In conclusion, the established screening protocol allowed us to identify mutations that affect plant protection, and it opens the possibility to uncover traits important for in planta microbe-microbe interactions.

  14. The Small Subunit of Snapdragon Geranyl Diphosphate Synthase Modifies the Chain Length Specificity of Tobacco Geranylgeranyl Diphosphate Synthase in Planta[W

    PubMed Central

    Orlova, Irina; Nagegowda, Dinesh A.; Kish, Christine M.; Gutensohn, Michael; Maeda, Hiroshi; Varbanova, Marina; Fridman, Eyal; Yamaguchi, Shinjiro; Hanada, Atsushi; Kamiya, Yuji; Krichevsky, Alexander; Citovsky, Vitaly; Pichersky, Eran; Dudareva, Natalia

    2009-01-01

    Geranyl diphosphate (GPP), the precursor of many monoterpene end products, is synthesized in plastids by a condensation of dimethylallyl diphosphate and isopentenyl diphosphate (IPP) in a reaction catalyzed by homodimeric or heterodimeric GPP synthase (GPPS). In the heterodimeric enzymes, a noncatalytic small subunit (GPPS.SSU) determines the product specificity of the catalytic large subunit, which may be either an active geranylgeranyl diphosphate synthase (GGPPS) or an inactive GGPPS-like protein. Here, we show that expression of snapdragon (Antirrhinum majus) GPPS.SSU in tobacco (Nicotiana tabacum) plants increased the total GPPS activity and monoterpene emission from leaves and flowers, indicating that the introduced catalytically inactive GPPS.SSU found endogenous large subunit partner(s) and formed an active snapdragon/tobacco GPPS in planta. Bimolecular fluorescence complementation and in vitro enzyme analysis of individual and hybrid proteins revealed that two of four GGPPS-like candidates from tobacco EST databases encode bona fide GGPPS that can interact with snapdragon GPPS.SSU and form a functional GPPS enzyme in plastids. The formation of chimeric GPPS in transgenic plants also resulted in leaf chlorosis, increased light sensitivity, and dwarfism due to decreased levels of chlorophylls, carotenoids, and gibberellins. In addition, these transgenic plants had reduced levels of sesquiterpene emission, suggesting that the export of isoprenoid intermediates from the plastids into the cytosol was decreased. These results provide genetic evidence that GPPS.SSU modifies the chain length specificity of phylogenetically distant GGPPS and can modulate IPP flux distribution between GPP and GGPP synthesis in planta. PMID:20028839

  15. Transient Expression of Secretory IgA In Planta is Optimal Using a Multi-Gene Vector and may be Further Enhanced by Improving Joining Chain Incorporation

    PubMed Central

    Westerhof, Lotte B.; Wilbers, Ruud H. P.; van Raaij, Debbie R.; van Wijk, Christina Z.; Goverse, Aska; Bakker, Jaap; Schots, Arjen

    2016-01-01

    Secretory IgA (sIgA) is a crucial antibody in host defense at mucosal surfaces. It is a promising antibody isotype in a variety of therapeutic settings such as passive vaccination and treatment of inflammatory disorders. However, heterologous production of this heteromultimeric protein complex is still suboptimal. The challenge is the coordinate expression of the four required polypeptides; the alpha heavy chain, the light chain, the joining chain, and part of the polymeric-Ig-receptor called the secretory component, in a 4:4:1:1 ratio. We evaluated the transient expression of three sIgAκ variants, harboring the heavy chain isotype α1, α2m1, or α2m2, of the clinical antibody Ustekinumab in planta. Ustekinumab is directed against the p40 subunit that is shared by the pro-inflammatory cytokines interleukin (IL)-12 and IL-23. A sIgA variant of this antibody may enable localized treatment of inflammatory bowel disease. Of the three different sIgA variants we obtained the highest yield with sIgA1κ reaching up to 373 μg sIgA/mg total soluble protein. The use of a multi-cassette vector containing all four expression cassettes was most efficient. However, not the expression strategy, but the incorporation of the joining chain turned out to be the limiting step for sIgA production. Our data demonstrate that transient expression in planta is suitable for the economic production of heteromultimeric protein complexes such as sIgA. PMID:26793201

  16. Forward Genetic In Planta Screen for Identification of Plant-Protective Traits of Sphingomonas sp. Strain Fr1 against Pseudomonas syringae DC3000

    PubMed Central

    Vogel, Christine; Innerebner, Gerd; Zingg, Judith; Guder, Jan

    2012-01-01

    Sphingomonas sp. strain Fr1 has recently been shown to protect Arabidopsis thaliana against the bacterial leaf pathogen Pseudomonas syringae DC3000. Here, we describe a forward genetic in planta screen to identify genes in Sphingomonas sp. Fr1 necessary for this effect. About 5,000 Sphingomonas sp. Fr1 mini-Tn5 mutants were assayed for a defect in plant protection against a luxCDABE-tagged P. syringae DC3000 derivative in a space-saving 24-well plate system. The bioluminescence of the pathogen was used as the indicator of pathogen proliferation and allowed for the identification of Sphingomonas sp. Fr1 mutants that had lost the ability to restrict pathogen growth before disease symptoms were visible. Potential candidates were validated using the same miniaturized experimental system. Of these mutants, 10 were confirmed as plant protection defective yet colonization competent. The mutants were subsequently evaluated in a previously described standard microbox system, and plants showed enhanced disease phenotypes after pathogen infection relative to those inoculated with the parental strain as a control. However, the disease severities were lower than those observed for control plants that were grown axenically prior to pathogen challenge, which suggests that several traits may contribute to plant protection. Transposon insertion sites of validated mutants with defects in plant protection were determined and mapped to 7 distinct genomic regions. In conclusion, the established screening protocol allowed us to identify mutations that affect plant protection, and it opens the possibility to uncover traits important for in planta microbe-microbe interactions. PMID:22660707

  17. Genetic modification of potato against microbial diseases: in vitro and in planta activity of a dermaseptin B1 derivative, MsrA2.

    PubMed

    Osusky, Milan; Osuska, Lubica; Kay, William; Misra, Santosh

    2005-08-01

    Dermaseptin B1 is a potent cationic antimicrobial peptide found in skin secretions of the arboreal frog Phyllomedusa bicolor. A synthetic derivative of dermaseptin B1, MsrA2 (N-Met-dermaseptin B1), elicited strong antimicrobial activities against various phytopathogenic fungi and bacteria in vitro. To assess its potential for plant protection, MsrA2 was expressed at low levels (1-5 microg/g of fresh tissue) in the transgenic potato (Solanum tuberosum L.) cv. Desiree. Stringent challenges of these transgenic potato plants with a variety of highly virulent fungal phytopathogens--Alternaria, Cercospora, Fusarium, Phytophthora, Pythium, Rhizoctonia and Verticillium species--and with the bacterial pathogen Erwinia carotovora demonstrated that the plants had an unusually broad-spectrum and powerful resistance to infection. MsrA2 profoundly protected both plants and tubers from diseases such as late blight, dry rot and pink rot and markedly extended the storage life of tubers. Due to these properties in planta, MsrA2 is proposed as an ideal antimicrobial peptide candidate to significantly increase resistance to phytopathogens and improve quality in a variety of crops worldwide with the potential to obviate fungicides and facilitate storage under difficult conditions.

  18. In Planta Biocontrol of Pectobacterium atrosepticum by Rhodococcus erythropolis Involves Silencing of Pathogen Communication by the Rhodococcal Gamma-Lactone Catabolic Pathway.

    PubMed

    Barbey, Corinne; Crépin, Alexandre; Bergeau, Dorian; Ouchiha, Asma; Mijouin, Lily; Taupin, Laure; Orange, Nicole; Feuilloley, Marc; Dufour, Alain; Burini, Jean-François; Latour, Xavier

    2013-01-01

    The virulence of numerous Gram-negative bacteria is under the control of a quorum sensing process based on synthesis and perception of N-acyl homoserine lactones. Rhodococcus erythropolis, a Gram-positive bacterium, has recently been proposed as a biocontrol agent for plant protection against soft-rot bacteria, including Pectobacterium. Here, we show that the γ-lactone catabolic pathway of R. erythropolis disrupts Pectobacterium communication and prevents plant soft-rot. We report the first characterization and demonstration of N-acyl homoserine lactone quenching in planta. In particular, we describe the transcription of the R. erythropolis lactonase gene, encoding the key enzyme of this pathway, and the subsequent lactone breakdown. The role of this catabolic pathway in biocontrol activity was confirmed by deletion of the lactonase gene from R. erythropolis and also its heterologous expression in Escherichia coli. The γ-lactone catabolic pathway is induced by pathogen communication rather than by pathogen invasion. This is thus a novel and unusual biocontrol pathway, differing from those previously described as protecting plants from phytopathogens. These findings also suggest the existence of an additional pathway contributing to plant protection.

  19. Pea lectin receptor-like kinase promotes high salinity stress tolerance in bacteria and expresses in response to stress in planta.

    PubMed

    Joshi, Amita; Dang, Hung Quang; Vaid, Neha; Tuteja, Narendra

    2010-01-01

    The plant lectin receptor-like kinases (LecRLKs) are involved in various signaling pathways but their role in salinity stress tolerance has not heretofore been well described. Salinity stress negatively affects plant growth/productivity and threatens food security worldwide. Based on functional gene-mining assay, we have isolated 34 salinity tolerant genes out of one million Escherichia coli (SOLR) transformants containing pea cDNAs grown in 0.8 M NaCl. Sequence analysis of one of these revealed homology to LecRLK, which possesses N-myristilation and N-glycosylation sites thus corroborating the protein to be a glycoconjugate. The homology based computational modeling of the kinase domain suggested high degree of conservation with the protein already known to be stress responsive in plants. The NaCl tolerance provided by PsLecRLK to the above bacteria was further confirmed in E. coli (DH5alpha). In planta studies showed that the expression of PsLecRLK cDNA was significantly upregulated in response to NaCl as compared to K(+) and Li(+) ions, suggesting the Na(+) ion specific response. Transcript of the PsLecRLK gene accumulates mainly in roots and shoots. The purified 47 kDa recombinant PsLecRLK-KD (kinase domain) protein has been shown to phosphorylate general substrates like MBP and casein. This study not only suggests the conservation of the cellular response to high salinity stress across prokaryotes and plant kingdom but also provides impetus to develop novel concepts for better understanding of mechanism of stress tolerance in bacteria and plants. It also opens up new avenues for studying practical aspects of plant salinity tolerance for enhanced agricultural productivity.

  20. Boosting In Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity

    PubMed Central

    Piron, Robin; De Koker, Stefaan; De Paepe, Annelies; Goossens, Julie; Grooten, Johan; Nauwynck, Hans; Depicker, Ann

    2014-01-01

    Porcine reproductive and respiratory syndrome (PRRS) is a disease of swine, caused by an arterivirus, the PRRS virus (PRRSV). This virus infects pigs worldwide and causes huge economic losses. Due to genetic drift, current vaccines are losing their power. Adaptable vaccines could provide a solution to this problem. This study aims at producing in planta a set of antigens derived from the PRRSV glycoproteins (GPs) to be included in a subunit vaccine. We selected the GP3, GP4 and GP5 and optimized these for production in an Arabidopsis seed platform by removing transmembrane domains (Tm) and/or adding stabilizing protein domains, such as the green fluorescent protein (GFP) and immunoglobulin (IgG) ‘Fragment crystallizable’ (Fc) chains. Accumulation of the GPs with and without Tm was low, reaching no more than 0.10% of total soluble protein (TSP) in homozygous seed. However, addition of stabilizing domains boosted accumulation up to a maximum of 2.74% of TSP when GFP was used, and albeit less effectively, also the Fc chains of the porcine IgG3 and murine IgG2a increased antigen accumulation, to 0.96% and 1.81% of TSP respectively, while the murine IgG3 Fc chain did not. Antigens with Tm were less susceptible to these manipulations to increase yield. All antigens were produced in the endoplasmic reticulum and accordingly, they carried high-mannose N-glycans. The immunogenicity of several of those antigens was assessed and we show that vaccination with purified antigens did elicit the production of antibodies with virus neutralizing activity in mice but not in pigs. PMID:24614617

  1. Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV) and subsequent evaluation of their immunogenicity.

    PubMed

    Piron, Robin; De Koker, Stefaan; De Paepe, Annelies; Goossens, Julie; Grooten, Johan; Nauwynck, Hans; Depicker, Ann

    2014-01-01

    Porcine reproductive and respiratory syndrome (PRRS) is a disease of swine, caused by an arterivirus, the PRRS virus (PRRSV). This virus infects pigs worldwide and causes huge economic losses. Due to genetic drift, current vaccines are losing their power. Adaptable vaccines could provide a solution to this problem. This study aims at producing in planta a set of antigens derived from the PRRSV glycoproteins (GPs) to be included in a subunit vaccine. We selected the GP3, GP4 and GP5 and optimized these for production in an Arabidopsis seed platform by removing transmembrane domains (Tm) and/or adding stabilizing protein domains, such as the green fluorescent protein (GFP) and immunoglobulin (IgG) 'Fragment crystallizable' (Fc) chains. Accumulation of the GPs with and without Tm was low, reaching no more than 0.10% of total soluble protein (TSP) in homozygous seed. However, addition of stabilizing domains boosted accumulation up to a maximum of 2.74% of TSP when GFP was used, and albeit less effectively, also the Fc chains of the porcine IgG3 and murine IgG2a increased antigen accumulation, to 0.96% and 1.81% of TSP respectively, while the murine IgG3 Fc chain did not. Antigens with Tm were less susceptible to these manipulations to increase yield. All antigens were produced in the endoplasmic reticulum and accordingly, they carried high-mannose N-glycans. The immunogenicity of several of those antigens was assessed and we show that vaccination with purified antigens did elicit the production of antibodies with virus neutralizing activity in mice but not in pigs.

  2. In Planta Expression Screens of Phytophthora infestans RXLR Effectors Reveal Diverse Phenotypes, Including Activation of the Solanum bulbocastanum Disease Resistance Protein Rpi-blb2[W

    PubMed Central

    Oh, Sang-Keun; Young, Carolyn; Lee, Minkyoung; Oliva, Ricardo; Bozkurt, Tolga O.; Cano, Liliana M.; Win, Joe; Bos, Jorunn I.B.; Liu, Hsin-Yin; van Damme, Mireille; Morgan, William; Choi, Doil; Van der Vossen, Edwin A.G.; Vleeshouwers, Vivianne G.A.A.; Kamoun, Sophien

    2009-01-01

    The Irish potato famine pathogen Phytophthora infestans is predicted to secrete hundreds of effector proteins. To address the challenge of assigning biological functions to computationally predicted effector genes, we combined allele mining with high-throughput in planta expression. We developed a library of 62 infection-ready P. infestans RXLR effector clones, obtained using primer pairs corresponding to 32 genes and assigned activities to several of these genes. This approach revealed that 16 of the 62 examined effectors cause phenotypes when expressed inside plant cells. Besides the well-studied AVR3a effector, two additional effectors, PexRD8 and PexRD3645-1, suppressed the hypersensitive cell death triggered by the elicitin INF1, another secreted protein of P. infestans. One effector, PexRD2, promoted cell death in Nicotiana benthamiana and other solanaceous plants. Finally, two families of effectors induced hypersensitive cell death specifically in the presence of the Solanum bulbocastanum late blight resistance genes Rpi-blb1 and Rpi-blb2, thereby exhibiting the activities expected for Avrblb1 and Avrblb2. The AVRblb2 family was then studied in more detail and found to be highly variable and under diversifying selection in P. infestans. Structure-function experiments indicated that a 34–amino acid region in the C-terminal half of AVRblb2 is sufficient for triggering Rpi-blb2 hypersensitivity and that a single positively selected AVRblb2 residue is critical for recognition by Rpi-blb2. PMID:19794118

  3. La problematica de la demarcacion entre ciencia y pseudociencia y sus implicaciones en la educacion cientifica

    NASA Astrophysics Data System (ADS)

    Jimenez Tolentino, Dinorah

    2011-12-01

    En la sociedad prevalece una tendencia generalizada hacia la inclusion de creencias y practicas pseudocientificas. Esta investigacion responde a la necesidad de analizar como la proliferacion de las pseudociencias afecta la vision que tienen los estudiantes universitarios sobre las ciencias naturales. A tales efectos, la investigadora describe las concepciones epistemologicas que tienen los estudiantes sobre las ciencias y las pseudociencias e identifica los criterios de demarcacion, entre un area y otra, que se derivan de estas concepciones. De igual modo, esta identifica las creencias y practicas pseudocientificas de mayor arraigo entre los estudiantes, destacando, a su vez, la razon de ser de las mismas. Por ultimo, la investigadora analiza las implicaciones educativas de la problematica de la demarcacion entre ciencia y pseudociencia. La investigacion es de naturaleza mixta, enmarcada en los paradigmas empirico- analitico y cualitativo. El proceso investigativo se llevo a cabo mediante la administracion del cuestionario Criterios para la demarcacion entre ciencia y pseudociencia. La parte cualitativa estuvo enmarcada en el diseno de estudio de caso, recopilando informacion mediante entrevistas semiestructuradas en dos grupos focales. La poblacion de estudio estuvo constituida por estudiantes universitarios del nivel subgraduado de la Universidad Central de Bayamon. Los resultados del estudio reflejaron las concepciones erroneas de los estudiantes sobre la naturaleza de las ciencias y las pseudociencias. Con respecto a la demarcacion entre ciencia y pseudociencia, el criterio imperante entre los universitarios es el de la verificabilidad, considerando la aplicacion del metodo cientifico como el metodo para demostrar la veracidad de las teorias cientificas. Las creencias y practicas pseudocientificas no son muy frecuentes entre los universitarios. Estos atribuyen las mismas a la prevalencia de elementos supersticiosos y al engano a que es sometida la poblacion

  4. Entre Dos Mundos/Between Two Worlds: Youth Violence Prevention for Acculturating Latino Families

    ERIC Educational Resources Information Center

    Smokowski, Paul R.; Bacallao, Martica

    2009-01-01

    Objective: This study evaluated the efficacy of Entre Dos Mundos/Between Two Worlds (EDM) prevention for Latino adolescents. Method: In an experimental trial to compare implementation formats, 41 Latino families were randomly assigned to EDM action-oriented skills training groups, and 47 families were randomly assigned to unstructured EDM support…

  5. Tdp studies and tests for C. A. Energia Electrica de Venezuela (enelven) at planta ramon laguna, units RL-17 and RL-10. Volume 2. Unit RL-10 boiler condition assessment report. Export trade information

    SciTech Connect

    Not Available

    1991-03-28

    The study, conducted by Babcock and Wilcox, was funded by the U.S. Trade and Development agency on behalf of Enelven. In order to maximize generated power output and minimize operating costs at Planta Ramon Laguna, tests were done to evaluate the condition of equipment at the plant. In order to identify any damage and determine the operating output of each unit, assessments were done of the furnaces, boilers, generators and boiler feed pumps being used in the plant. The report presents the results of these tests. This is the second of three volumes and it includes the following section: (1) Condition Assessment of Unit RL-10 Boiler.

  6. [Agrobacterium-mediated sunflower transformation (Helianthus annuus L.) in vitro and in Planta using strain of LBA4404 harboring binary vector pBi2E with dsRNA-suppressor proline dehydrogenase gene].

    PubMed

    Tishchenko, E N; Komisarenko, A G; Mikhal'skaia, S I; Sergeeva, L E; Adamenko, N I; Morgun, B V; Kochetov, A V

    2014-01-01

    To estimate the efficiency of proline dehydrogenase gene suppression towards increasing of sunflower (Helianthus annuus L.) tolerance level to water deficit and salinity, we employed strain LBA4404 harboring pBi2E with double-stranded RNA-suppressor, which were prepared on basis arabidopsis ProDH1 gene. The techniques of Agrobacterium-mediated transformation in vitro and in planta during fertilization sunflower have been proposed. There was shown the genotype-depended integration of T-DNA in sunflower genome. PCR-analysis showed that ProDH1 presents in genome of inbred lines transformed in planta, as well as in T1- and T2-generations. In trans-genic regenerants the essential accumulation of free L-proline during early stages of in vitro cultivation under normal conditions was shown. There was established the essential accumulation of free proline in transgenic regenerants during cultivation under lethal stress pressure (0.4 M mannitol and 2.0% sea water salts) and its decline upon the recovery period. These data are declared about effectiveness of suppression of sunflower ProDH and gene participation in processes connected with osmotolerance.

  7. ESTIGMA Y VIH/SIDA ENTRE PADRES/MADRES Y ADOLESCENTES PUERTORRIQUEÑOS/AS

    PubMed Central

    Pérez, Grace Rosado; Reyes, Glendalys Rivera; Villanueva, Victoria Larrieux; Torres, Gilliam J. Torres; Díaz, Elba Betancourt; Varas-Díaz, Nelson; Villaruel, Antonia

    2016-01-01

    La comunicación entre padres/madres y adolescentes sobre el tema de la sexualidad es importante para el desarrollo de la salud de personas jóvenes. Dicha comunicación puede verse negativamente impactada por actitudes estigmatizantes hacia el tema del VIH/SIDA. El objetivo de este estudio fue identificar actitudes estigmatizantes hacia el VIH/SIDA entre padres/madres y adolescentes puertorriqueños/as. Este esfuerzo es parte del Proyecto Cuídalos, dirigido a probar una intervención en formato electrónico que busca aumentar la comunicación sobre sexualidad y salud entre padres/madres y adolescentes mediante un diseño experimental con 458 diadas de padres/madres y adolescentes de 13 a 17 años. Para propósitos de este artículo reportamos estadísticas descriptivas sobre estigma hacia el VIH/SIDA con la información recopilada en la medición basal. Tanto adultos/as como adolescentes mostraron actitudes estigmatizantes hacia el VIH/SIDA. A la luz de los resultados es necesario continuar desarrollando intervenciones para la reducción de estigma en esta población. Los/as padres/madres pueden ser un recurso invaluable para reducir el estigma en los/as jóvenes, y prevenir conductas sexuales de riesgo e infecciones. PMID:27099649

  8. Tdp studies and tests for C. A. Energia Electrica de Venezuela (enelven) at planta ramon laguna, units RL-17 and RL-10. Volume 1. Executive summary, RL-17 test report, and gas conversion proposals. Export trade information

    SciTech Connect

    Not Available

    1991-03-28

    The study, conducted by Babcock and Wilcox, was funded by the U.S. Trade and Development agency on behalf of Enelven. In order to maximize generated power output and minimize operating costs at Planta Ramon Laguna, tests were done to evaluate the condition of equipment at the plant. In order to identify any damage and determine the operating output of each unit, assessments were done of the furnaces, boilers, generators and boiler feed pumps being used in the plant. The report presents the results of these tests. This is the first of three volumes and it is divided into the following sections: (1) Executive Summary; (2) Hydrogen Damage Assessment; (3) RL-17 Gas Conversion Proposal; (4) RL-10 and RL-11 Gas Conversion Proposals.

  9. A full-length infectious clone of beet soil-borne virus indicates the dispensability of the RNA-2 for virus survival in planta and symptom expression on Chenopodium quinoa leaves.

    PubMed

    Crutzen, François; Mehrvar, Mohsen; Gilmer, David; Bragard, Claude

    2009-12-01

    For a better understanding of the functionality and pathogenicity of beet soil-borne virus (BSBV), full-length cDNA clones have been constructed for the three genomic RNAs. With the aim of assessing their effectiveness and relative contribution to the virus housekeeping functions, transcripts were inoculated on Chenopodium quinoa and Beta macrocarpa leaves using five genome combinations. Both RNAs-1 (putative replicase) and -3 (putative movement proteins) proved to be essential for virus replication in planta and symptom production on C. quinoa, whereas RNA-2 (putative coat protein, CP, and a read-through domain, RT) was not. No symptoms were recorded on B. macrocarpa, but viral RNAs were detected. In both host plants, the 19 kDa CP was detected by Western blotting as well as a 115 kDa protein corresponding to the CP-RT.

  10. Estudo comparativo entre estrelas centrais de nebulosas planetárias deficientes em hidrogênio

    NASA Astrophysics Data System (ADS)

    Marcolino, W. L. F.; de Araújo, F. X.

    2003-08-01

    Apresentamos neste trabalho o resultado de um estudo das principais características espectrais das estrelas centrais de nebulosas planetárias (ECNP) deficientes em hidrogênio. A origem e a evolução dessas estrelas ainda constitui um problema em aberto na evolução estelar. Geralmente esses objetos são divididos em [WCE], [WCL] e [WELS]. Os tipos [WCE] e [WCL] apresentam um espectro típico de uma estrela Wolf-Rayet carbonada de população I e as [WELS] apresentam linhas fracas de carbono e oxigênio em emissão. Existem evidências que apontam a seguinte sequência evolutiva : [WCL] = > [WCE] = > [WELS] = > PG 1159 (pré anã-branca). No entanto, tal cenário apresenta falhas como por exemplo a falta de ECNP entre os tipos [WCL] e [WCE]. Baseados em uma amostra de 24 objetos obtida no telescópio de 1.52m em La Silla, Chile (acordo ESO/ON), ao longo do ano 2000, apresentamos os resultados da comparação das larguras equivalentes de diversas linhas relevantes entre os tipos [WCL], [WCE] e [WELS]. Verificamos que nossos dados estão de acordo com a sequência evolutiva. Baseado nas linhas de C IV, conseguimos dividir pela primeira vez as [WELS] em dois grupos principais. Além disso, os dados reforçam a afirmação de que as [WCE] são as estrelas que possuem a maior temperatura entre as ECNP deficientes em hidrogênio. Discutimos ainda, a escassez de dados disponíveis na literatura e a necessidade da obtenção de parametros físicos para estes objetos.

  11. Relaciones entre el sueño y la adicción

    PubMed Central

    Cañellas, Francesca; de Lecea, Luis

    2016-01-01

    Resumen La interacción entre los trastornos del sueño y el abuso de sustancias es ya conocida, pero seguramente más compleja de lo que se pensaba. Existe tanto una relación positiva entre tener un trastorno por uso de substancias y sufrir un trastorno de sueño, como viceversa. Los efectos sobre el sueño dependen de la substancia utilizada, pero se ha demostrado que tanto durante su uso como en período de abstinencia los consumidores tienen diferentes problemas de sueño y fundamentalmente un sueño más fragmentado. Sabemos que hay que tener en cuenta los problemas de sueño para evitar recaídas en la adicción. Investigaciones recientes indican que el sistema hipocretinérgico definido por el neuropéptido hipocretina/orexina (Hcrt/ox), localizado en el hipotálamo lateral e implicado entre otros en la regulación del ciclo sueño-vigilia, jugaría un papel importante en las conductas adictivas. Diferentes estudios han demostrado interacciones entre el sistema hipocretinérgico, los circuitos de respuesta aguda al estrés y los sistemas de recompensa. También sabemos que la activación optogenética selectiva del sistema hipocretinérgico incrementa la probabilidad de la transición del sueño a la vigilia, y también es suficiente para iniciar un comportamiento compulsivo de recaída adictiva. La activación del sistema hipocretinérgico podría explicar la hipervigilia asociada al estrés y a la adicción. El mayor conocimiento de esta interacción permitiría entender mejor los mecanismos de la adicción y encontrar nuevas estrategias para el tratamiento de las adicciones. PMID:23241715

  12. Transfert radiatif entre une petite particule et un diélectrique: application au chauffage local

    NASA Astrophysics Data System (ADS)

    Mulet, J.-P.; Joulain, K.; Carminati, R.; Greffet, J. J.

    2002-06-01

    nous montrons dans cette étude que le transfert radiatif entre une particule de taille nanométrique et un diélectrique petit être très important lorsque les distances mises en jeu sont petites devant la longueur d'onde caractéristique du rayonnement thermique. Ce transfert peut devenir dominant lorsque les matériaux utilisés sont polaires. Nous discuterons de la possibilité d'appliquer ces résultats au chauffage local dans les nano-structures.

  13. [Presence of botulinum-producing clostridia in the soils of Entre Ríos].

    PubMed

    Lúquez, C; Fernández, R A; Bianco, M I; de Jong, L I T; Saldaño, V; Ciccarelli, A S

    2003-01-01

    Botulism, well known for centuries, continues to preoccupy the Public Health authorities, food and agricultural industries. In its oldest form it results from ingestion of food containing botulinum toxin. To correctly evaluate the incidence of botulism it is fundamental to know the distribution of botulinum toxin-producing clostridia (Clostridium botulinum, C. baratii, C. butyricum and C. argentinense) in nature, specially in the soil, its main reservoir. In our country, this study has been carried out in a partial way. With the aim to contribute to such knowledge, 240 soil samples from Entre Ríos province were examined for C. botulinum and 35 (14.6%) samples resulted positive.

  14. [Phlebotominae: vectors of leishmaniasis in the provinces of Santa Fe and Entre Ríos, Argentina].

    PubMed

    Salomón, Oscar D; Mocarbel, Nicolás J; Pedroni, Elena; Colombo, Javier; Sandillú, Mónica

    2006-01-01

    The transmission of tegumentary leishmaniasis (TL) has increased in 9 provinces of Argentina since 1985. Santa Fe and Entre Ríos did not record in this period autochtonous probed cases: however, an epidemic outbreak took place in 2003 in Bella Vista, Corrientes, located in an area with ecological continuity and contiguous to both provinces. In order to evaluate the potential risk of transmission of LT, Phlebotominae were captured at locations close to and southern from Bella Vista during February 2004. The traps located on the shores of Parana river in Santa Fe (El Rabón, Villa Ocampo, Cayastá), and Entre Ríos (La Paz. La Celina-Villa Urquiza) captured 860 individuals of Lutzomyia neivai (99.5%) and Lu. migonei (0.5 %), both species with vectorial capacity for Leishmania (V.) braziliensis. In Tartagal, Santa Fe, the captures were consistent with the residual "chaco" landscape, 7 individuals of Lu. nerivai, Lu. migonei and Lu. cortelezzii. The risk of LT epidemic transmission in these provinces is highlighted, mainly due to the progressive southern tropicalization of the paranaense gallery forest. Clinical and entomological surveillance is recommended.

  15. Miroirs pour l'UV lointain (VUV) entre 80 et 130 nm

    NASA Astrophysics Data System (ADS)

    Bridou, F.; Cuniot-Ponsard, M.; Desvignes, J.-M.

    2006-12-01

    Dans le domaine spectral entre 80 et 130 nm situé dans le VUV (“Vacuum Ultra-Violet”), les matériaux ne sont ni transparents, ni vraiment réfléchissants. Il est difficile de réaliser des systèmes optiques et, en conséquence, de déterminer les constantes optiques des matériaux. Les écarts entre la réflectivité calculée à partir des indices référencés dans les tables et la réflectivité expérimentale nous ont conduits à penser que certains de ces indices n'étaient pas exacts. Nous avons développé une méthode expérimentale de détermination des constantes optiques à partir des mesures de réflectivité au voisinage de l'incidence normale, en fonction de la longueur d'onde. A partir des valeurs ainsi déterminées, des composants tels que miroirs ou polariseurs peuvent être conçus et réalisés.

  16. An Environmental Management Model of Thermal Waters in Entre Ríos Province, Argentina

    NASA Astrophysics Data System (ADS)

    Pablo, Mársico Daniel; Luís, Díaz Eduardo; Ivana, Zecca; Oscar, Dallacosta; Antonio, Paz-González

    2015-04-01

    Deep exploratory drillings, i.e. those with more than 500 meters depth, have been performed in the Entre Ríos province, Argentina, in order to ascertain the presence of thermal water. Drilling began in 1994, and until now there have been 18 polls with very variable results in terms of mineralization, resource flow, and temperature. The aim of this study was to present a management model, which should allow operators of thermal complexes to further develop procedures for safeguarding the biodiversity of the ecosystems involved, both during exploration and exploitation activities. The environmental management Plan proposed is constituted by a set of technical procedures that are formulated and should be performed during the stages of exploration and exploitation of the resource, and consists of: environmental monitoring, environmental audit, public information and contingency programs. This Plan describes the measures and proposals aimed at protecting environmental quality in the area of influence of a thermal complex project, ensuring that its execution remains environmentally responsibly, and allowing implementation of specific actions to prevent or correct environmental impacts, as predicted in the evaluation of the Environmental Program. The audit of environmental impact includes and takes into account natural factors, such as water, soil, atmosphere, flora and fauna, and also cultural factors. The technical audit Plan was prepared in order to get a systematic structure and organization of the verification process, and also with regard to document the degree of implementation of the proposed mitigation measures. Finally, an environmental contingency program was implemented, and its objective was to consider the safeguarding of life and its natural environment. Thus, a guide has been developed with the main actions to be taken on a contingency, since forecast increases the efficiency of the response. The methodology developed here was adopted as the procedure

  17. An updated model for nitrate uptake modelling in plants. I. Functional component: cross-combination of flow–force interpretation of nitrate uptake isotherms, and environmental and in planta regulation of nitrate influx

    PubMed Central

    Le Deunff, Erwan; Malagoli, Philippe

    2014-01-01

    Background and Aims In spite of major breakthroughs in the last three decades in the identification of root nitrate uptake transporters in plants and the associated regulation of nitrate transport activities, a simplified and operational modelling approach for nitrate uptake is still lacking. This is due mainly to the difficulty in linking the various regulations of nitrate transport that act at different levels of time and on different spatial scales. Methods A cross-combination of a Flow–Force approach applied to nitrate influx isotherms and experimentally determined environmental and in planta regulation is used to model nitrate in oilseed rape, Brassica napus. In contrast to ‘Enzyme–Substrate’ interpretations, a Flow–Force modelling approach considers the root as a single catalytic structure and does not infer hypothetical cellular processes among nitrate transporter activities across cellular layers in the mature roots. In addition, this approach accounts for the driving force on ion transport based on the gradient of electrochemical potential, which is more appropriate from a thermodynamic viewpoint. Key Results and Conclusions Use of a Flow–Force formalism on nitrate influx isotherms leads to the development of a new conceptual mechanistic basis to model more accurately N uptake by a winter oilseed rape crop under field conditions during the whole growth cycle. This forms the functional component of a proposed new structure–function mechanistic model of N uptake. PMID:24638820

  18. Religiosidade, juventude e sexualidade: entre a autonomia e a rigidez1

    PubMed Central

    Silva, Cristiane Gonçalves da; Santos, Alessandro Oliveira; Licciardi, Daniele Carli; Paiva, Vera; Parker, Richard

    2009-01-01

    Esse artigo descreve como jovens religiosos e autoridades religiosas de sua comunidade compreendem a sexualidade, considerando suas experiências pessoais e como membros de comunidades religiosas. A análise pretende contribuir para que políticas públicas dedicadas à promoção da saúde sexual da juventude considerem a religiosidade, no contexto de um estado laico e da promoção do direito à prevenção. Foram realizadas 26 entrevistas abertas e semidirigidas em diferentes comunidades da região metropolitana da cidade de São Paulo (comunidades católicas, da umbanda, do candomblé e de diferentes denominações evangélicas) sobre iniciação sexual, casamento, gravidez, contracepção e prevenção das DST/Aids, homossexualidade, aborto e direitos humanos. Observou-se como jovens e autoridades religiosas convivem com a tensão entre tradição e modernidade e os distintos discursos sobre a sexualidade. Como sujeitos religiosos (do discurso religioso) e sujeitos sexuais (de discursos sobre sexualidade), devem ser incorporados pelos programas como sujeitos de direito nos termos de sua religiosidade. PMID:21886456

  19. El espectro de KX TrA entre 1990 y 1996

    NASA Astrophysics Data System (ADS)

    Brandi, E.; García, L.; Ferrer, O.; Barbá, R.

    La estrella simbiótica KX TrA = He2-177 fue observada espectroscópicamente con el telescopio de 2.15 m del CASLEO entre los años 1990 y 1996, utilizándose resoluciones intermedia y alta. El rango espectral estudiado se extiende desde 4400 Åa 7200 Å. La historia fotométrica de KX TrA presenta explosiones del mismo tipo que las mostradas por la nova lenta RR Tel y su espectro de alta excitación, muy rico en líneas de emisión, es también similar al de RR Tel. Por lo tanto, es importante analizar la evolución espectral de KX TrA en el tiempo, prestando especial atención a los posibles cambios en los niveles de excitación. Las emisiones presentes corresponden a transiciones permitidas y prohibidas con un amplio rango de ionización, incluyéndose las anchas e intensas líneas originadas por scattering Raman de O VI en λλ 6825 y 7082 Å. En el período cubierto por nuestras observaciones se estudia la variación de la intensidad relativa de las emisiones, el comportamiento de las velocidades radiales y los cambios de perfiles de las líneas, especialmente en Hα y Hβ. El contínuo de la región roja observada indica un tipo espectral no más tardío que M3 para la componente gigante fría del sistema.

  20. Analyse des interactions energetiques entre un arena et son systeme de refrigeration

    NASA Astrophysics Data System (ADS)

    Seghouani, Lotfi

    La presente these s'inscrit dans le cadre d'un projet strategique sur les arenas finance par le CRSNG (Conseil de Recherche en Sciences Naturelles et en Genie du Canada) qui a pour but principal le developpement d'un outil numerique capable d'estimer et d'optimiser la consommation d'energie dans les arenas et curlings. Notre travail s'inscrit comme une suite a un travail deja realise par DAOUD et coll. (2006, 2007) qui a developpe un modele 3D (AIM) en regime transitoire de l'arena Camilien Houde a Montreal et qui calcule les flux de chaleur a travers l'enveloppe du batiment ainsi que les distributions de temperatures et d'humidite durant une annee meteorologique typique. En particulier, il calcule les flux de chaleur a travers la couche de glace dus a la convection, la radiation et la condensation. Dans un premier temps nous avons developpe un modele de la structure sous la glace (BIM) qui tient compte de sa geometrie 3D, des differentes couches, de l'effet transitoire, des gains de chaleur du sol en dessous et autour de l'arena etudie ainsi que de la temperature d'entree de la saumure dans la dalle de beton. Par la suite le BIM a ete couple le AIM. Dans la deuxieme etape, nous avons developpe un modele du systeme de refrigeration (REFSYS) en regime quasi-permanent pour l'arena etudie sur la base d'une combinaison de relations thermodynamiques, de correlations de transfert de chaleur et de relations elaborees a partir de donnees disponibles dans le catalogue du manufacturier. Enfin le couplage final entre l'AIM +BIM et le REFSYS a ete effectue sous l'interface du logiciel TRNSYS. Plusieurs etudes parametriques on ete entreprises pour evaluer les effets du climat, de la temperature de la saumure, de l'epaisseur de la glace, etc. sur la consommation energetique de l'arena. Aussi, quelques strategies pour diminuer cette consommation ont ete etudiees. Le considerable potentiel de recuperation de chaleur au niveau des condenseurs qui peut reduire l'energie requise par

  1. Seroprevalence of Salmonella and Mycoplasma infection in backyard chickens in the state of Entre Rios in Argentina.

    PubMed

    Xavier, J; Pascal, D; Crespo, E; Schell, H L; Trinidad, J A; Bueno, D J

    2011-04-01

    The present work was conducted to study the seroprevalence of Salmonella, Mycoplasma gallisepticum (MG), and Mycoplasma synoviae (MS) infection in backyard chickens located in Entre Ríos, Argentina, over 3 periods of time. A total of 2,441 sera samples were collected from backyard chickens belonging to 256 family farms in 16 counties in the state of Entre Ríos from January to May 2003 (first period), December 2004 to April 2005 (second period), and October 2006 to May 2007 (third period). The prevalence of family farms testing seropositive for Salmonella averaged 23.9, 15.9, and 28.6% during the first, second, and third period, respectively. The highest prevalence of Salmonella-seropositive farms recorded (66.7%) was on farms from Concordia county, and the lowest prevalence (0%) was on farms from La Paz county. In contrast, the prevalence of family farms seropositive for MG averaged 32.8, 55.1, and 76.2% during the first, second, and third periods, respectively. The highest prevalence of MG-seropositive farms (100%) was found in the counties of Victoria and Tala, and the lowest prevalence (8.7%) was found on farms on Colón county. The prevalence of family farms seropositive for MS averaged 68.6 and 100% during the first and second periods, respectively. The highest prevalence of MS-seropositive farms (100%) was on farms in 85% of the counties tested, and the lowest prevalence (21.7%) was on farms from Colón county. Salmonella, MG, and MS infection are present at high levels in backyard chicken farms, and this presents a high risk to commercial poultry production in Entre Ríos, the state with the highest chicken population and density in Argentina.

  2. Servicio de Mapas en Internet para la Salud Ambiental en la Region Fronteriza Entre los Estados Unidos y Mexico

    USGS Publications Warehouse

    Buckler, Denny; Stefanov, Jim

    2004-01-01

    La region fronteriza de los Estados Unidos y Mexico abarca una gran diversidad de ambientes fisicos y habitaciones, entre los cuales estan los humedales, desiertos, pastos, montanas, y bosques. Estos a su vez son unicos en cuanto a su diversidad de recursos acuaticos minerales, y biologicos. La region se interconecta economica, politica, y socialmente debido a su herencia binacional. En 1995, cerca de 11 millones de habitantes vivian en la zona adyacente a la frontera. Un estudio sugiere que esa poblacion podria doblarse antes del ano 2020.

  3. Etude de la transition tribologique entre le fretting et le meso-fretting pour des materiaux de contact electrique

    NASA Astrophysics Data System (ADS)

    Gagnon, Daniel

    Dans les installations electriques, les contacts sont toujours soumis a des contraintes alternees ou a des micro-deplacements. Il en resulte une corrosion par fretting, phenomene defini comme un type de deterioration de la surface qui se produit lorsque deux materiaux en contact sont soumis a des mouvements microscopiques d'oscillations de tres faible amplitude (0 a 100 mum). Ceci a pour effet de provoquer une degradation de la resistance de contact et une interruption du passage du courant. Ce phenomene a des repercussions considerables sur le plan pratique puisque les micro-deplacements de pieces en contact peuvent etre causes par la dilatation thermique differentielle des metaux, par des vibrations mecaniques, par la relaxation des contraintes ou par l'echauffement des contacts lorsqu'on interrompt et retablit le courant. Nous avons donc dans le cadre de cette these etudie plusieurs aspects du fretting (0--100 mum) et du meso-fretting (100 a 1000 mum) pour differents materiaux de contact electrique. Des travaux experimentaux ont ete realises a partir de deux montages reproduisant divers aspects de la degradation par le fretting. Un premier montage de fretting de type bille-plaque a ete entierement developpe a l'ETS et un second montage, de type fil-plaque a ete utilise en collaboration avec Hydro Quebec IREQ a Varennes. Plusieurs techniques de mesures et d'analyse relevant tant du domaine de la mecanique du contact que de la metallurgie ont ete utilisees pour traiter les resultats. L'influence du courant sur le taux d'usure et la force de friction a ete examinee pour divers materiaux de contacts. Des essais de fatigue thermique et electrique ont ete realises sur divers materiaux et lubrifiants de contact. Il a ete demontre que pour le domaine entre 100 mum et 1000 mum, le taux d'usure n'est pas le meme de 0 a 100 mum et au dela de 1000 mum. La plupart des materiaux evalues montrent un stade de comportement intermediaire dont le debut se situe entre 100 mum et

  4. Clostridium perfringens growth from spore inocula in sous-vide processed pork-based Mexican entrée.

    PubMed

    Miguel-Garcia, Denise Y; Juneja, Vijay K; Valenzuela-Melendrez, Martin; Díaz-Cinco, Martha E; Thippareddi, H; Aida Peña-Ramos, E

    2009-01-01

    The combined effect of Citricidal wih irradiation on Clostridium perfringens growth from spores in a sous-vide processed marinated pork meat Mexican entrée was investigated. Citricidal was added at 200 or 800 ppm after mixing pork meat with tomatillo sauce and inoculated with 3 log(10) CFU/g of C. perfringens spores. Samples were irradiated at either 0 or 2 kGy, heated to an internal temperature of 71 degrees C, and stored at 4 degrees C for 28 d, 15 degrees C for 45 d, and 25 degrees C for 26 h. To simulate the conditions that may occur during transportation, distribution, storage, or handling in supermarkets or by consumers, the effect of static temperature abuse on C. perfringens growth was assessed by transferring samples stored at 4 to 25 degrees C for 13 and 15 h. Total C. perfringens populations were determined by plating diluted samples on tryptose-sulfite-cycloserine agar. Growth was not observed up to 45 d of storage at 15 degrees C in samples supplemented with 800 ppm of Citricidal. At 25 degrees C, no significant differences (P > 0.05) on the lag phase duration due to antimicrobial treatments was observed. The temperature abuse of refrigerated products for up to 15 h did not lead to C. perfringens growth to high infective dose levels of 1 million cells required to cause food poisoning. The results suggest that 800 ppm Citricidal can have significant bacteriostatic activity against C. perfringens and may provide a degree of protection against this pathogen in sous-vide processed marinated pork meat Mexican entrée, under mild temperature abuse (

  5. Tdp studies and tests for C. A. Energia Electrica de Venezuela (enelven) at planta ramon laguna, units RL-17 and RL-10. Volume 3. Unit RL-10 turbine generator condition assessment report and units RL-10 and RL-11 boiler feed pump conditon assessment report. Export trade information

    SciTech Connect

    Not Available

    1991-03-28

    The study, conducted by Babcock and Wilcox, was funded by the U.S. Trade and Development agency on behalf of Enelven. In order to maximize generated power output and minimize operating costs at Planta Ramon Laguna, tests were done to evaluate the condition of equipment at the plant. In order to identify any damage and determine the operating output of each unit, assessments were done of the furnaces, boilers, generators and boiler feed pumps being used in the plant. The report presents the results of these tests. This is the last of three volumes and it is divided into the following sections: (1) Condition Assessment of Unit RL-10 Turbine-Generator; (2) Condition Assessment of Unit RL-10 and RL-11 Boiler Feed Pumps.

  6. Soil Quality Indicators to Define Land Use in the Area of Native Forest of Entre Ríos, Argentina

    NASA Astrophysics Data System (ADS)

    Wilson, M. G.; Tasi, H. A.; Paz González, A.; Díaz, E. L.; Sasal, M. C.

    2012-04-01

    The main economic activity in the area of native forest of the province of Entre Ríos (Argentina) has long been the agricultural and/or livestock production, especially cattle breeding. In recent years, the proportion of agricultural crops in the rotations, especially that of soybean, has increased, thus leading to an increase in the need for land clearing to incorporate new lands for agricultural use. Most of these lands are considered marginal for agricultural use. In addition rice farming with irrigation is a critical part of the Entre Ríos economy. Defining and assessing soil quality indicators (SQI) that show the evolution of the soil with different uses and management systems is a way to contribute to the knowledge of soil quality. The aims of this study were to characterize the current land use and land tenure in the area of native forest of Entre Ríos, as well as to identify and select variables sensitive to agricultural and/or livestock use of the most representative soils of this area (indicators of the dynamic quality of the soil) and define the most appropriate land use according to land suitability and behavior of these indicators. We identified the most representative soil subgroups (corresponding to the orders Vertisols, Mollisols and Alfisols) and defined the production systems livestock-agricultural, agricultural-livestock, agricultural without irrigation, and rice crop irrigated with water from groundwater and surface reservoirs. We also determined the physical, physico-chemical, chemical and microbiological variables of the soil, and characterized the quality of the water for irrigation. We selected the SQI using Principal Components Analysis, to form a minimum data set (MDS). The change in the use of the land responded to a favorable economic situation for agriculture that started in the 1990's. The leasing and sharecropping schemes and the incidental contracts have become increasingly important, predominating over the undivided property. We

  7. State of Conservation of the Native Forests in Entre Ríos (Argentina) and Changes in Land Use

    NASA Astrophysics Data System (ADS)

    Sabattini, R. A.; Sione, S. M.; Ledesma, S. G.; Sabattini, J. A.; Wilson, M. G.

    2012-04-01

    The native forest area of Entre Ríos province (Argentina) is associated with a constant change in land use, with an increase in recent years in agricultural use, especially for soybean crop. In addition, since its inadequate management has triggered degradation processes of the natural forest structure, the implementation of strategies for the restoration and conservation of native forests has become a priority. The aim of this study was to diagnose the conservation state of the native forest in the basin of the Estacas Stream (Entre Ríos, Argentina) after the change in land use, to help design guidelines for the restoration and sustainable management of these ecosystems. The field study was conducted in October 2010, in a representative area of the native forest of 73,000 ha. Using Landsat 5-TM images (INPE), environments were separated by manual vectorization, identifying and classifying native forests and other lands (agricultural, urban). Using a field exploratory survey (58 geo-referenced sampling points), we developed patterns corresponding to the different types of forests, contrasting this information with the digital data of the images. The native forests were classified according to type (high/low forest, open/closed forest, savanna), successional stage (climax, successional or regeneration forest) and degree of disturbance (weed growth, erosion, fire), and their frequency determined. Each classification was assessed by a contingency matrix, and global reliability index and the Kappa index. The information obtained generated a classification map of native forests in the basin scale. We found that the native forest covered an area of 42,726.91 ha, accounting for 58.52% of the total basin area, and that the rest corresponded to other land uses. The most frequent native forests (59.09%) were climax forest, but accounted for only 8.2% of the basin area. Within this group, the most important were the low and open forest, with Prosopis affinis and Prosopis

  8. Effect of grapefruit seed extract on thermal inactivation of Listeria monocytogenes during sous-vide processing of two marinated Mexican meat entrées

    Technology Transfer Automated Retrieval System (TEKTRAN)

    D and z values of Listeria monocytogenes were obtained for two Mexican meat entrées: pork meat marinated in tomatillo (green tomato) sauce (PTS) and beef marinated in a red chili sauce (BRCS), with addition of 0, 200 and 800 ppm of grapefruit seed extract (GSE). Meat samples, inoculated with L.monoc...

  9. A Process Evaluation of an Efficacious Family-Based Intervention to Promote Healthy Eating: The "Entre Familia: Reflejos de Salud" Study

    ERIC Educational Resources Information Center

    Schmied, Emily; Parada, Humberto; Horton, Lucy; Ibarra, Leticia; Ayala, Guadalupe

    2015-01-01

    "Entre Familia: Reflejos de Salud" was a successful family-based randomized controlled trial designed to improve dietary behaviors and intake among U.S. Latino families, specifically fruit and vegetable intake. The novel intervention design merged a community health worker ("promotora") model with an entertainment-education…

  10. Análisis de la correlación espacio-temporal entre ondas EIT y ondas Moreton

    NASA Astrophysics Data System (ADS)

    Borgazzi, A.; Rovira, M.; Stenborg, G.

    Observaciones de la atmósfera solar en distintas longitudes de onda han demostrado la presencia de fenómenos ondulatorios, tanto a nivel cromosférico como coronal. En el primero de los casos a las ondas observadas se las dió por llamar ondas Moreton, mientras que en el segundo ondas EIT. En el presente trabajo se abordará el estudio de la posible relación causal entre ambos fenómenos ondulatorios. Para concretar dicha meta se hará uso de un conjunto de observaciones del disco solar en Hα (para la determinación y análisis de las ondas Moreton) y en Fe XII (para la determinación y análisis de las ondas EIT). Los observables que se midan serán luego usados para la interpretación física del fenómeno en base a modelos teóricos existentes.

  11. On acoplamento mecânico entre a antena e o transdutor no detector de ondas gravitacionais Mario Schenberg

    NASA Astrophysics Data System (ADS)

    Melo, J. L.; Aguiar, O. D.; Velloso, W. F., Jr.; Lucena, A. U.

    2003-08-01

    O detector de ondas gravitacionais MARIO SCHENBERG consistirá de uma massa esférica de cobre-alumínio de 1150kg resfriada a 4K, sobre a qual serão instalados 6 transdutores de nióbio. Com estes trandutores pretende-se converter um possível sinal de onda gravitacional detectado em sinal elétrico, para tanto é necessário que o acoplamento mecânico entre os transdutores e a massa ressonante seja o maior possível. Isto significa que o transdutor deve ser ressonante na mesma freqüência que a antena (aproximadamente 3200Hz). Neste trabalho foi desenvolvida uma geometria para a estrutura mecânica do trandutor. Isto foi feito criando-se modelos em elementos finitos usando-se o "software" MSC/Nastran. Estes modelos criados foram analisados estaticamente (cálculo de tensões) e dinamicamente (cálculo das freqüências de ressonâncias e seus respectivos modos normais) de maneira a se obter o primeiro modo normal do transdutor em 3200Hz. A partir destes cálculos escolheu-se a melhor geometria para o transdutor. Os próximos passos do trabalho serão: usinar este transdutor em uma barra de nióbio e testá-lo à temperatura ambiente e à baixa temperatura. Após isto, pretende-se testá-lo na própria antena resfriada.

  12. [The record of sensitive nerve action potentials. Interelectrode distance in healthy children].

    PubMed

    Arango-Aguilar, Jaime; Fraire-Martínez, María Inés; Sepúlveda-Vildósola, Ana Ana

    2014-01-01

    INTRODUCCIÓN: estudios en adultos muestran que con 3 o 4 cm de distancia entre los electrodos activo y de referencia se obtienen los valores ma´ximos de amplitud y latencia de los potenciales de accio´n nerviosos sensitivos. El objetivo de este estudio fue identificar en niños sanos, las distancia entre los electrodos con la cual los valores de amplitud y latencia pico no difieren significativamente de los obtenidos con la distancia entre los electrodos a la cual se obtienen los valores máximos. ME´TODOS: se colocaron cuatro electrodos de referencia a 1, 2, 3 y 4 cm del electrodo activo. La señal del electrodo activo se registró simultáneamente con un puente físico entre los cuatro canales. Se analizó una muestra de 66 nervios medianos en niños de cinco a nueve años. Los valores máximos de amplitud y latencia se obtuvieron con 4 cm entre los electrodos. Se compararon los valores obtenidos con 4 cm entre los electrodos y con 1, 2 y 3 cm.

  13. Half-size me? How calorie and price information influence ordering on restaurant menus with both half and full entrée portion sizes.

    PubMed

    Haws, Kelly L; Liu, Peggy J

    2016-02-01

    Many restaurants are increasingly required to display calorie information on their menus. We present a study examining how consumers' food choices are affected by the presence of calorie information on restaurant menus. However, unlike prior research on this topic, we focus on the effect of calorie information on food choices made from a menu that contains both full size portions and half size portions of entrées. This different focus is important because many restaurants increasingly provide more than one portion size option per entrée. Additionally, we examine whether the impact of calorie information differs depending on whether full portions are cheaper per unit than half portions (non-linear pricing) or whether they have a similar per unit price (linear pricing). We find that when linear pricing is used, calorie information leads people to order fewer calories. This decrease occurs as people switch from unhealthy full sized portions to healthy full sized portions, not to unhealthy half sized portions. In contrast, when non-linear pricing is used, calorie information has no impact on calories selected. Considering the impact of calorie information on consumers' choices from menus with more than one entrée portion size option is increasingly important given restaurant and legislative trends, and the present research demonstrates that calorie information and pricing scheme may interact to affect choices from such menus.

  14. Cristallisation, syncristallisation, et alliages moléculaires entre le lorazépam et l'oxazépam

    NASA Astrophysics Data System (ADS)

    Mascherpa-Corral, D.; Mascherpa, G.; Chauvet, A.

    1993-04-01

    Le polymorphisme et pseudopolymorphisme du lorazépam et oxazépam ont été étudiés par analyse thermique, calorimétrie différentielle à balayage et diffraction de rayons X. Deux formes polymorphes du lorazépam et plusieurs mono et hemisolvates ont été isolés à partir de recristallisation dans divers soivants. Aucun polymorphisme ni solvate n'a été mis en évidence avec l'oxazépam. Les paramètres cristallographiques des phases isolées ont été déterminées. A 160 et 203°C, le lorazépam et l'oxazépam perdent respectivement une molécule d'eau pour donner après réarrangement la quinazolinecarboxaldéhyde correspondante. La syncristallisation des deux benzodiazépines dans le benzène conduit à des alliages moléculaires à miscibilité totale à l'état solide dans tout le domaine de concentration entre l'exazépam el la forme α du lorazépam, elle n'est que partielle avec la forme β. Thermal analysis, differential scanning calorimetry, and X-ray diffraction were carried out to study the polymorphism and pseudopolymorphism of lorazepam and oxazepam. Two polymorphic forms of lorazepam and several mono- or hemisolvates were obtained after recrystallization from various solvents. No polymorphic form or solvate has been found for oxazepam. The crystallographic parameters of these new phases were determined. Lorazepam and oxazepam can lose a molecule of water at 160 and 203°C, respectively, and rearrange to quinazolinecarboxaldehyde. Mixed crystals of the two benzodiazepines, after recrystallization from benzene, lead to molecular alloys with complete solid solubility between oxazepam and the α-form of lorazepam over the whole range of composition but only to partial solubility with the β-form of lorazepam.

  15. Distancias, cinemática y distribución galáctica de estrellas B[e

    NASA Astrophysics Data System (ADS)

    Zorec, J.

    A group of O, B and early A spectral type stars has the generic name B[e]. They are characterized by strong hydrogen emission lines, visible forbidden emission lines and strong infrarred excess. The B[e] phenomenon concerns objects with different characteristics and they can be in different evolutionary stages. Hence, we cannot consider them forming a uniform stellar group. This phenomenon is observed in Herbig AeBe-like stars, supergiants, hypergiants, stars ressembling pre-planetary nebulae or symbiotics or even in objects which seem to be on or near the main sequence. Most of them have a distance that cannot be obtained by direct methods. We can estimate them by indirect methods only. In this contribution we present an indirect method of distance determination of B[e] stars which take into account their most outstanding spectrophotometric characteristics: flux excesses/defficiencies and variations produced by their gaseous and dusty components of the circumstellar envelope. We also discuss the way to estimate the interstellar absorption affecting the energy distribution of these objects. The dispersion of peculiar galactic velocities of B[e] stars and their galactic distribution suggest that these objects are however of extreme Population I.

  16. The Amount of Time to Eat Lunch is Associated with Children’s Selection and Consumption of School Meal Entrée, Fruits, Vegetable, and Milk

    PubMed Central

    Cohen, Juliana F.W.; Jahn, Jaquelyn L.; Richardson, Scott; Cluggish, Sarah A.; Parker, Ellen; Rimm, Eric B.

    2015-01-01

    Background There are currently no national standards for school lunch period lengths and little is known about the association between the amount of time students have to eat and school food selection and consumption. Objectives To examine plate waste measurements from students in the control arm from the MEALS study (2011-2012 school year) for the association of the amount of time to eat with school meal selection and consumption. Design Prospective study using up to six repeated measures among students over the school year. Participants/Setting 1001 students in grades 3-8 attending 6 participating elementary/middle schools in an urban, low-income school district where lunch period lengths varied from 20-30 minutes. Main Outcome Measures School food selection and consumption were collected using plate waste methodology. Statistical Analyses Performed Logistic regression and mixed-model ANOVA was used to examine food selection and consumption. Results Compared with meal component selection when students had at least 25 minutes to eat, students were significantly less likely to select a fruit (44% vs. 57%; p=0.0001) compared with when students had fewer than 20 minutes to eat. There were no significant differences in entrée, milk, or vegetable selection. Among those who selected a meal component, students with fewer than 20 minutes to eat consumed 13% less of their entrée (p<0.0001), 10% less of their milk (p<0.0001), and 12% less of their vegetable (p=0.0002) compared to when students had at least 25 minutes to eat. Conclusions Over the school year, a substantial number of students had insufficient time to eat, which was associated with significantly decreased entrée, milk, and vegetable consumption compared with students who had more time to eat. School policies that encourage lunches with at least 25 minutes of seated time may reduce food waste and improve dietary intake. PMID:26372337

  17. Relation entre les caractéristiques des table-bancs et les mesures anthropométriques des écoliers au Benin

    PubMed Central

    Falola, Stève Marjelin; Gouthon, Polycarpe; Falola, Jean-Marie; Fiogbe, Michel Armand; Nigan, Issiako Bio

    2014-01-01

    Introduction Le mobilier scolaire et la posture assise en classe sont souvent impliqués dans l'apparition des douleurs rachidiennes, influant de fait sur la qualité des tâches réalisées par les apprenants. Aucune étude n'a encore vérifié le degré d'adéquation entre les caractéristiques du mobilier et celles des écoliers au Bénin. L'objectif de cette étude transversale est donc de déterminer la relation entre les dimensions des table-bancs utilisées en classe et les mesures anthropométriques des écoliers au Bénin. Methods Elle a été réalisée avec un échantillon probabiliste de 678 écoliers, âgés de 4 à 17 ans. Les mesures anthropométriques des écoliers et les mensurations relatives aux longueurs, largeurs et hauteurs des table-bancs ont été mesurées, puis intégrées aux équations proposées dans la littérature. Les pourcentages des valeurs situées hors des limitesacceptables, dérivées de l'application des équations ont été calculés. Results La largeur et la hauteur des table-bancs utilisées par les écoliers étaient plus élevées (p < 0,05) que les valeurs de référence recommandées par les structures officielles de contrôle et de production des mobiliers scolaires au Bénin. Quel que soit le sexe, il y avait une inadéquation entre la largeur du banc et la longueur fesse-poplité, puis entre la hauteur de la table et la distance coude-bancdes écoliers. Conclusion Les résultats suggèrent de prendre en compte l’évolution des mesures anthropométriques des écoliers dans la confection des table-bancs, afin de promouvoir de bonnes postures assises en classe et de réduire le risque de troubles du rachis. PMID:25317232

  18. [Pilot study of the acoustic values of the vowels in Spanish as indicators of the severity of dysarthria].

    PubMed

    Delgado-Hernandez, J

    2017-02-01

    Introduccion. El analisis acustico es una herramienta que proporciona datos objetivos sobre las alteraciones del habla en la disartria. Objetivos. Evaluar en la disartria ataxica la relacion del nivel de inteligibilidad del habla con el area del espacio vocalico (VSA), la ratio de centralizacion de los dos primeros formantes (FCR) y la media de las distancias primarias. Sujetos y metodos. Se utilizo una muestra de 14 hablantes de español, 10 con disartria y cuatro controles. Se analizaron los valores del primer y segundo formante en 140 vocales extraidas de 140 palabras. Para calcular el nivel de inteligibilidad participaron siete oyentes y se utilizo una tarea de identificacion de estimulos verbales. Resultados. Los sujetos disartricos tienen un menor contraste entre las vocales medias y altas y entre las vocales posteriores. Se observan diferencias significativas del VSA, la FCR y la media de las distancias primarias respecto a los sujetos controles (p = 0,007, 0,005 y 0,030, respectivamente). Los analisis de regresion muestran la relacion del VSA y la media de las distancias primarias con el nivel de inteligibilidad del habla (r = 0,60 y 0,74, respectivamente). Conclusiones. Los sujetos con disartria ataxica presentan un menor contraste vocalico y una centralizacion en la realizacion de las vocales. Las medidas acusticas estudiadas en este trabajo preliminar tienen una alta sensibilidad en la deteccion de la disartria, pero solo el VSA y la media de las distancias primarias informan sobre la gravedad de este tipo de alteracion del habla.

  19. Effect of Grapefruit Seed Extract on Thermal Inactivation of Listeria monocytogenes during Sous-Vide Processing of Two Marinated Mexican Meat Entrées.

    PubMed

    Valenzuela-Melendres, Martin; Peña-Ramos, E Aida; Juneja, Vijay K; Camou, Juan Pedro; Cumplido-Barbeitia, German

    2016-07-01

    D- and z-values for Listeria monocytogenes were obtained for two Mexican meat entrées: pork meat marinated in tomatillo (green tomato) sauce (PTS) and beef marinated in a red chili sauce (BRCS), with addition of 0, 200, and 800 ppm of grapefruit seed extract (GSE). Meat samples inoculated with L. monocytogenes were packaged in sterile bags, immersed in a water bath, and held at 55, 57.5, 60, and 62.5°C for different periods of time. Depending upon the temperature, D-values at 0 ppm of GSE ranged from 26.19 to 2.03 min in BRCS and 26.41 to 0.8 min in PTS. Adding 800 ppm of GSE to BRCS thermally treated at 55 and 62.5°C significantly decreased inactivation time by 35%. A reduction in time of 25.9, 10.6, and 40.1% at 55, 57.5, and 60°C, respectively, was observed in PTS with 800 ppm of GSE. The z-values of L. monocytogenes were not significantly affected by GSE addition; average z-values were 7.25 and 5.09°C for BRCS and PTS, respectively. Estimated thermal lethality for a 7-D log reduction of L. monocytogenes under commercial-size sous-vide conditions at a reference temperature of 55°C was reached at 78 and 71 min for BRCS without and with 800 ppm of GSE, respectively. For PTS, 7-D reduction was attained at 69 and 61 min without and with addition of 800 ppm of GSE, respectively. Supplementing both Mexican meat entrées (BRCS and PTS) with 800 ppm of GSE rendered L. monocytogenes cells more sensitive to the lethal effect of heat. The results of this study will assist the retail food industry in designing acceptance limits on critical control points pertaining to cooking regimes to effectively eliminate L. monocytogenes in BRCS and PTS sous-vide processed Mexican meat entrées.

  20. Amorçage d'une décharge dans le vide entre deux electrodes d'argent ou des alliages argent-nickel

    NASA Astrophysics Data System (ADS)

    Zouache, N.; Lefort, A.

    1997-03-01

    Comparison of the characteristics of an electric are breakdown in vacuum between two silver or silver-nickel alloy electrodes prompted us to study the evolution of the breakdown voltage with electrode separation, with resistance value placed between the anode and the high voltage supply used for the breakdown, and with the effect of conditionning by heating in vacuum. The measurement of the commutation time and delay time, and the observations by a metallographic microscope of the electrodes surfaces after one breakdown, enabled us to evidence the origin of the electric arc for each of the studied materials. La comparaison des caractéristiques de l'amorçage d'un arc électrique dans le vide entre deux électrodes bombées de 8mm de diamètre et de rayon de courbure en surface de 16mm, constituées d'argent ou de son alliage avec le nickel, nous a amené à étudier l'évolution de la tension d'amorçage avec plusieurs paramètres : la distance inter-électrodes, les valeurs de la résistance intercalée entre la source haute tension servant au claquage et les électrodes et le conditionnement par chauffage sous vide des électrodes. La mesure du temps de commutation, du temps de retard et les observations au microscope métallographique de la surface des électrodes après un amorçage donnent des informations sur l'origine de l'arc électrique concernant chaque matériau étudié.

  1. Selective Effect of Pesticides on Plant--A Review.

    PubMed

    Parween, Talat; Jan, Sumira; Mahmooduzzafar, Sumira; Fatma, Tasneem; Siddiqui, Zahid Hameed

    2016-01-01

    This review represents systematic and integrated picture of pesticide exposure to plant and its effect on growth and metabolism. Decades ago, agrochemicals were introduced aiming at enhancing crop yields and protecting crops from pests. Due to adaptation and resistance developed by pests to chemicals, every year higher amounts and new chemical compounds are used to protect crops, causing undesired side effects and raising the costs of food production. Biological chemical free agriculture is gaining also more and more support but it is still not able to respond to the need for producing massive amounts of food. The use of agrochemicals, including pesticides, remains a common practice especially in tropical regions and South countries. Cheap compounds, such as DDT, HCH, and Lindane, that are environmentally persistent, are today banned from agriculture use in developed countries, but remain popular in developing countries. As a consequence, persistent residues of these chemicals contaminate food and disperse in the environment. Therefore, the thrust of this paper was to review the application of pesticides effect early from germination to growth of the plant, leading to alteration in biochemical, physiological and different enzymatic and non-enzymatic antioxidants which ultimately affect the yield and resulted in residues in plant, vegetables, and fruits.

  2. Pesticidas Naturais Derivdos de Plantas: Descoberta et Usos

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This review discusses the chemical diversity of natural products and the possibility of isolating active compounds for pest management using bioassays. There is currently a demand for more effective and less expensive natural products for pest management. The approval process for natural products as...

  3. From planta to pharma with glycosylation in the toolbox.

    PubMed

    Saint-Jore-Dupas, Claude; Faye, Loïc; Gomord, Véronique

    2007-07-01

    Plant-specific glycosylation has long been a major limitation to the extensive use of plant-made pharmaceuticals in human therapy. Our goal here is to highlight the progress recently made towards humanization of N-glycosylation in plants and to illustrate that plant-typical N- and O-glycosylation progressively emerge as additional advantages for using this promising expression system.

  4. A Process Evaluation of an Efficacious Family-Based Intervention to Promote Healthy Eating: The Entre Familia: Reflejos de Salud Study.

    PubMed

    Schmied, Emily; Parada, Humberto; Horton, Lucy; Ibarra, Leticia; Ayala, Guadalupe

    2015-10-01

    Entre Familia: Reflejos de Salud was a successful family-based randomized controlled trial designed to improve dietary behaviors and intake among U.S. Latino families, specifically fruit and vegetable intake. The novel intervention design merged a community health worker (promotora) model with an entertainment-education component. This process evaluation examined intervention implementation and assessed relationships between implementation factors and dietary change. Participants included 180 mothers randomized to an intervention condition. Process evaluation measures were obtained from participant interviews and promotora notes and included fidelity, dose delivered (i.e., minutes of promotora in-person contact with families, number of promotora home visits), and dose received (i.e., participant use of and satisfaction with intervention materials). Outcome variables included changes in vegetable intake and the use of behavioral strategies to increase dietary fiber and decrease dietary fat intake. Participant satisfaction was high, and fidelity was achieved; 87.5% of families received the planned number of promotora home visits. In the multivariable model, satisfaction with intervention materials predicted more frequent use of strategies to increase dietary fiber (p ≤ .01). Trends suggested that keeping families in the prescribed intervention timeline and obtaining support from other social network members through sharing of program materials may improve changes. Study findings elucidate the relationship between specific intervention processes and dietary changes.

  5. Uma Comparação entre Técnicas de Propagação de Erros em Astrofísica: Monte Carlo x Bootstrap

    NASA Astrophysics Data System (ADS)

    Zabot, Alexandre; Baptista, Raymundo

    2005-07-01

    Neste trabalho é feito um estudo comparativo entre dois algoritmos numéricos usados para propagação de erros em dados experimentais. Um deles é conhecido por Método de Monte carlo e o outro por Método de Bootstrap. Recentemente, Dhullon & Watson argüiram que a aplicação do método de Monte Carlo introduz ruído nos dados, e propuseram então a utilização do Bootstrap como alternativa capaz de produzir resultados superiores. O objetivo deste trabalho é testar a validade dessa afirmação. As duas técnicas foram aplicadas a três problemas diferentes: o ajsute de modelos de emissão LTE simples e atmosfera estelar a espectros estelares observados e o ajuste de curvas de luz de eclipses de Variáveis Cataclísmicas para a detemrinação da distribuição radial de brilho dos seus discos de acréscimo. Os métodos foram testados quanto à sua robusteza, ou seja, a capacidade de prover resultados coerentes enre si. Além disso, as soluções dos métodos foram comparadas. Os resultados indicam que não existe evidência de superioridade de um métodos em relação ao outro.

  6. Métastases cutanées vues au laboratoire d'anatomie pathologique à Lomé, Togo entre 2005 et 2014

    PubMed Central

    Darre, Tchin; Saka, Bayaki; Landoh, Dadja Essoya; Mouhari-Toure, Abas; Amegbor, Koffi; Pitché, Palokinam; Napo-Koura, Gado

    2015-01-01

    Introduction L'objectif de cette étude était de documenter le profil épidémiologique et histologique des métastases cutanées vues au laboratoire d'anatomie pathologique (LAP) de Lomé. Méthodes Il s'agissait d'une étude descriptive et transversale portant sur des cas de métastases cutanées observées au LAP du CHU Sylvanus Olympio entre 2005 et 2014. Résultats Au cours de cette période d’étude, nous avons recensé 32 cas de métastases cutanées représentant 3,2% (1005 cas) de l'ensemble des cas de métastases enregistrées au LAP. L’âge moyen des patients atteints de ces métastases cutanées était de 42,6 ans et le sex-ratio (F/H) de 2,2. Sur le plan macroscopique, ces métastases étaient nodulaires dans 15 cas, bourgeonnantes dans 12 cas, ulcéré dans 3 cas et ulcéro-bourgeonnant dans 2 cas. Les types histologiques étaient représentés par les adénocarcinomes (19 cas, 59,4%), les carcinomes épidermoïdes (8 cas, 25%), la maladie de Paget (3 cas, 9,4%), le carcinome à petites cellules du type neuroendocrine (un cas, 3,1%) et le mélanome (un cas, 3,1%). Selon le degré de différenciation, les métastases cutanées étaient bien différenciées dans 14 cas (56%). Les principales localisations de ces métastases cutanées étaient le thorax (11 cas, 34,4%) suivi de l'abdomen (10 cas, 31,3%). Les cancers primitifs étaient surtout d'origine mammaire (14 cas, 43,7%). Conclusion Cette étude montre que les métastases cutanées sont relativement rares et les tumeurs malignes d'origine mammaire sont les tumeurs primitives prédominantes. Avec un pourcentage élevé des formes peu différenciées et celles indifférenciées, l'amélioration du plateau technique du LAP (immunohistochimie) permettra d'accroitre ses capacités diagnostiques. PMID:27047627

  7. A Reappraising of Cosmography: the Interface Between Astronomical and Geographic Studies. (Breton Title: Releitura do Conceito de Cosmografia: a Interface Entre os Estudos Astronômicos e Geográficos.) Una Relectura del Concepto de Cosmografía: la Interfase Entre los Estudios Astronómicos y Geográficos

    NASA Astrophysics Data System (ADS)

    Azevedo Sobreira, Paulo Henrique

    2012-12-01

    The concept of "Cosmography" is in disuse since the 80s of the last century, but the astronomical themes previously discussed in the school subjects of Geography and Cosmography remain in current textbooks. The use of term "Cosmography" was rescued in this research, and the study of its re-signification prompted the appearance of the term Geographic Cosmography. The Geographic Cosmography is a field of studies of the Geography, whose set of knowledge and skills is predominantly scholar. It studies the interface between terrestrial and celestial knowledge, and assigns a geographic significance to them. It examines human and natural relationships with Sidereal Space and its consequences for society and nature. O conceito de "Cosmografia" está em desuso desde os anos 80 do século XX, mas os temas astronômicos anteriormente abordados nas disciplinas escolares de Cosmografia e de Geografia permanecem nos atuais livros didáticos. O uso do termo "Cosmografia" foi resgatado nesta pesquisa e o estudo de sua ressignificação proporcionou o surgimento do termo Cosmografia Geográfica. A Cosmografia Geográfica é um campo de estudos da Geografia, cujo conjunto de conhecimentos e habilidades é predominantemente escolar. Estuda a interface entre os conhecimentos terrestres e os celestes e lhes atribui significância geográfica. Analisa as relações humanas e naturais com o Espaço Sideral e suas consequências para a sociedade e a natureza.

    Aunque el concepto de "Cosmografía" no se usa desde la década de los '80 del siglo pasado, los temas astronómicos que se enseñaban anteriormente en las asignaturas escolares de Cosmografía y de Geografía permanecen en los actuales libros didácticos. El uso del término "Cosmografía" fue rescatado en esta investigación y el estudio de su resignificación proporcionó el surgimiento del término Cosmografía Geográfica. La Cosmografía Geográfica es un campo de estudio de la Geografía, donde

  8. Etude des phenomenes chimiques au contact entre le bloc cathodique et la barre collectrice d'une cellule d'electrolyse d'aluminium

    NASA Astrophysics Data System (ADS)

    Lebeuf, Martin

    La production d'aluminium est une industrie importante au Québec. Les propriétés de ce métal le vouent à de multiples usages présents et futurs dans le cadre d'une économie moderne durable. Toutefois, le procédé Hall-Héroult est très énergivore et des progrès demeurent donc nécessaires pour en diminuer les coûts financiers et environnementaux. Parmi les améliorations envisageables de la cellule d'électrolyse se trouve le contact entre la cathode et la barre collectrice, qui doit offrir une faible résistivité au passage du courant électrique. En cours d'opération de la cellule, ce contact a tendance à se dégrader, générant des pertes énergétiques significatives. Les causes de cette dégradation, pouvant provenir de phénomènes chimiques, thermiques, mécaniques et/ou électriques, demeurent mal comprises. Le but du présent projet était donc d'étudier les phénomènes chimiques se produisant au contact bloc-barre de la cellule d'électrolyse Hall-Héroult. En premier lieu, un aspect crucial à considérer est la pénétration du bain électrolytique dans la cathode, car des composés de bain atteignent éventuellement la barre collectrice et peuvent y réagir. A cet effet, une méthode novatrice a été développée afin d'étudier les cathodes et la pénétration du bain dans celles-ci à l'aide de la microtomographie à rayons X. Cette méthode rapide et efficace s'est avérée fort utile dans le projet et a un potentiel important pour l'étude future des cathodes et des phénomènes qui s'y produisent. Ensuite, une cellule d'électrolyse rectangulaire à petite échelle a été développée. Plusieurs phénomènes observés 'en industrie sur des autopsies de cellules post-opération et rapportés dans la littérature ont été reproduis avec succès à l'aide de cette cellule expérimentale. Puis, des tests sans électrolyse, ciblant l'effet du bain électrolytique sur l'acier, ont aussi été conçus et complétés afin de s

  9. Pincharse sin infectarse: estrategias para prevenir la infección por el VIH y el VHC entre usuarios de drogas inyectables

    PubMed Central

    MATEU-GELABERT, P.; FRIEDMAN, S.; SANDOVAL, M.

    2011-01-01

    infectarse por el VIH (sólo un doble negativo tuvo un amplio conocimiento sobre la hepatitis C). Estas intencionalidades no son mutuamente excluyentes. La presencia de varias refuerza la puesta en práctica a diario de comportamientos que pueden ayudar al que se inyecta a mantenerse libre de infecciones durante años. Algunas prácticas que hemos identificado se implementan en grupo y se comunican de UDI a UDI, de esta manera se extienden entre algunas redes sociales de UDI. Conclusiones Los UDI que permanecen sin infectarse planean e implementan estrategias de prevención en circunstancias donde otros UDI aplican prácticas de riesgo. El mantenimiento de la no infección no es, por lo tanto, un resultado del azar, sino más bien el resultado del esfuerzo (agencia) de los UDI. Investigar y extender estas estrategias y tácticas a través de programas de prevención podría contribuir a la prevención del VIH y el VHC. PMID:21915175

  10. Le renforcement de l'interface entre les services de santé publique et de première ligne : enjeux et pistes de solution

    PubMed Central

    Paquette, Daniel; Reinharz, Daniel

    2009-01-01

    Le développement de liens étroits entre la santé publique et les services cliniques, notamment ceux offerts par les médecins de famille œuvrant en première ligne, est prôné dans tous systèmes de santé occidentaux. On suppose que les collaborations formant une telle interface ont des impacts positifs à la fois sur la qualité des soins, sur le fardeau des maladies et sur l'équité dans le système de santé. Si les acteurs des deux domaines admettent le bien-fondé de l'interface et favorisent en principe son développement, plusieurs barrières nuisent aux collaborations dans la réalité. Or, peu est connu sur ces barrières dans le système de santé québécois. Cette étude vise à répondre à ce manque d'information en étudiant l'interface à partir d'une perspective organisationnelle. Deux cadres conceptuels ont été utilisés, soit la typologie de Lasker (1997) et les archétypes de Hinings et Greenwood (1988). L'analyse met en évidence deux des six formes de collaboration proposées par la typologie utilisée, soit la coordination des services aux individus et la mise à profit des rencontres cliniques pour les fins de la santé publique. Les activités associées à ces formes de collaboration, qui sont importantes eu égard au mandat de la santé publique, comportent un intérêt essentiellement pour la santé publique et sont déterminées en pratique unilatéralement par cette dernière. En outre, le manque de coordination des activités qui impliquent une collaboration avec les médecins constitue un obstacle au développement de telles activités en créant des irritants pour les médecins. Il y a donc, dans le système de santé québécois, des possibilités de développement de l'interface là où la collaboration se fait également au profit du travail clinique et là où elle tient compte des contraintes du milieu clinique. PMID:20436801

  11. La efectividad de la educacion a distancia como metodologia en la desarrollo de destrezas de pensamiento (Effectiveness of Distance Education as a Methodology for Developing Thinking Skills).

    ERIC Educational Resources Information Center

    Melendez Alicea, Juan

    1992-01-01

    Presents steps taken in designing, justifying, and implementing an experimental study designed to investigate the effectiveness of distance education as a methodology for developing thinking skills. A discussion reviews major findings of the study by comparing student experiences from multimedia distance education and student experiences from…

  12. El Programa de Educacion a Distancia de la Universidad Nacional Autonoma de Mexico (UNAM) (The Distance Education Program at the National Autonomous University of Mexico (UNAM)).

    ERIC Educational Resources Information Center

    Manilla, Jose Manuel Alvarez

    1996-01-01

    Describes the development of a distance education program at the National Autonomous University of Mexico (UNAM) which will meet several needs: lifelong learning; adult education; service to remote populations; and continuing education for faculty in remote campuses. Provision of large-scale education depends on addressing issues dealing with…

  13. Un modelo para el control de calidad academica de los textos de instruccion a distancia (A Model for Controlling the Academic Quality of Distance Education Texts).

    ERIC Educational Resources Information Center

    Bolanos-Mora, Guiselle; And Others

    1992-01-01

    In response to the need for a system of control over the academic quality of distance education texts, this article proposes a methodological model based on criteria that evaluate written materials based on their instructional quality, design, and production. A discussion and figures evaluate educational aspects of content, communication,…

  14. Le transfert de connaissances entre les mathematiques et les sciences. Une etude exploratoire aupres d'eleves de 4e secondaire

    NASA Astrophysics Data System (ADS)

    Samson, Ghislain

    2003-06-01

    Au moment ou dans plusieurs pays on travaille a refondre les programmes d'etudes, tant au primaire qu'au secondaire, l'interet pour le transfert renait. Un des concepts fondamentaux en apprentissage consiste en l'habilete a reutiliser de facon consciente et efficace un acquis d'une situation a une autre situation. Cette recherche emane de preoccupations professionnelles au moment ou le chercheur etait enseignant au secondaire. Au cours de ces annees, il lui a ete possible de constater que plusieurs eleves percevaient difficilement les liens presents entre les disciplines mathematiques et scientifiques. Des travaux en psychologie cognitive et plus particulierement selon une perspective du traitement de l'information ont servi de cadre de reference pour evaluer et analyser les capacites de transfert aupres d'eleves de 4e secondaire. Ce cadre de reference permet de formuler le principal objectif qui est de mieux comprendre le processus de transfert chez des eleves en situation de resolution de problemes scientifiques. Cette these s'interesse donc au transfert en tant que phenomene important du processus d'apprentissage au sens de l'integration. La methode de recherche choisie, de nature qualitative, est principalement axee sur l'evaluation de la capacite a transferer des connaissances lors d'une epreuve et d'un entretien. Pour evaluer ce potentiel de transfert, nous avons elabore deux outils: une epreuve en mathematiques et en sciences et un guide d'entretien. Pour la passation de l'epreuve, le chercheur a pu compter sur la collaboration de 130 sujets provenant de deux ecoles. L'entretien complete la prise de donnees avec 13 sujets ayant accepte de poursuivre l'etude. Les donnees recueillies par ces instruments font ensuite l'objet d'une analyse de contenu. En premier lieu, les verbatims de l'epreuve et de l'entretien ont ete transcrits, puis codifies. La correction des reponses fournies pour les problemes resolus s'est faite a partir d'une grille d

  15. [CRITERION-RELATED VALIDITY OF SIT-AND-REACH TEST AS A MEASURE OF HAMSTRING EXTENSIBILITY IN OLDER WOMEN].

    PubMed

    López-Miñarro, Pedro Ángel; Vaquero-Cristóbal, Raquel; Muyor, José María; Espejo-Antúnez, Luis

    2015-07-01

    Introducción: la disposición del raquis lumbo-sacro y la distancia alcanzada en el test sit-and-reach han sido propuestas para valorar la extensibilidad isquiosural, siendo su validez diferente en función de la población analizada. Objetivo: determinar la validez del ángulo lumbo-horizontal en flexión y la distancia alcanzada en el test sitand- reach como criterio de extensibilidad isquiosural en mujeres mayores. Metodología: un total de 120 mujeres mayores realizaron aleatoriamente los test de elevación de pierna recta (EPR) con ambas piernas y el test sit-and-reach (SR). En este último se valoró la distancia alcanzada y la disposición del raquis lumbo-sacro (L-Hfx) al alcanzar la posición de máxima flexión del tronco. Resultados y discusión: los valores medios en el EPR fueron 81,70 ± 13,83º y 82,10 ± 14,36º en las piernas izquierda y derecha, respectivamente. La media del EPR de ambas piernas fue de 81,90 ± 12,70º. La distancia media en el SR fue de -1,54 ± 8,09 cm. En el L-Hfx, el valor medio fue de 91,08º ± 9,32º. La correlación entre el test EPR medio de ambas piernas respecto a la distancia alcanzada en el test DDP y el ángulo L-Hfx fueron moderados (L-Hfx: r = -0,72, p < 0,01; SR: r = 0,70, p < 0,01). Ambas variables, de forma independiente, explicaron alrededor del 50% de la varianza (L-Hfx: R2 = 0,52; p < 0,001; SR: R2 = 0,49; p < 0,001). Conclusiones: la validez de la disposición del raquis lumbo-sacro en mujeres mayores es moderada, siendo similar a la obtenida por la distancia alcanzada en el test sit-and-reach.

  16. Association entre les hormones sexuelles, les marqueurs de remodelage osseux et la densité minérale osseuse chez des femmes ménopausées d'origine marocaine (étude transversale)

    PubMed Central

    El Maataoui, Aissam; Biaz, Asmae; El Boukhrissi, Fatima; El Machtani, Si; Dami, Abdellah; Bouhsain, Sanae; Bamou, Youssef; El Maghraoui, Abdellah; Ouzzif, Zhor

    2015-01-01

    Introduction Le présent travail se propose d’étudier la relation entre les hormones sexuelles, notamment l’œstradiol et l'indice de l’œstradiol libre, le sulfate de déhydroépiandrosterone et la sex hormone binding globulin, les marqueurs de remodelage osseux et la densité minérale osseuse chez une population de femmes marocaine ménopausées. Méthodes Il s'agit d'une étude transversale, menée sur une période de 6 mois d'octobre 2012 à fin avril 2013 et ayant fait participer deux cent deux (202). Résultats L’œstradiol et l'indice d’œstradiol libre (IEL) ont montré une corrélation négative respectivement à l'ostéocalcine (OC), à la crosslaps (β-CTX) et l'OC (p<0.001). La sulfate de déhydroepiandrosterone S-DHEA a été corrélée positivement à l'OC (p<0.001), alors que e taux sérique de la sex hormone binding globulin (SHBG) l'a été corrélé à la β-CTX et l'OC (p<0.001). Par ailleurs, une corrélation positive a été établie entre la densité minérale osseuse (DMO) au col de fémur et le poids, l'indice de masse corporelle (IMC), l'IEL et la S-DHEA. Une corrélation négative a été retrouvée entre la DMO au col de fémur d'une part et l’âge, la DDR, la SHBG, la β-CTX et l'OC d'une autre part. Conclusion Le présent travail montre que l'augmentation de l’âge et la diminution de l’œstradiol libre expliquent la diminution de la DMO au niveau du col du fémur, alors que l'augmentation du taux sérique de la SHBG et la diminution du poids expliquent la diminution de la DMO au rachis lombaire. PMID:26848353

  17. [Surnames and isonymy in the Garifuna communities of the Atlantic Coast of Honduras].

    PubMed

    Herrera-Paz, Edwin Francisco

    2013-01-01

    Introducción: la estimación de la isonimia es una herramienta importante en la determinación de la estructura genética de las comunidades puesto que su magnitud es aproximadamente igual a cuatro veces el coeficiente de endogamia FST. Métodos: se analizaron los apellidos registrados en la base de datos de 26 comunidades del Tribunal Supremo Electoral de Honduras. Se calculó la isonimia aleatoria dentro de cada comunidad y en el total, así como diversos parámetros comunes asociados. Se determinaron las distancias genéticas de Lasker a partir de la isonimia calculada entre pares de comunidades. Resultados: se encontraron valores relativamente altos de isonimia aleatoria en comparación con otras comunidades rurales. Adicionalmente, se observaron distancias genéticas relativamente cortas y se encontró una correlación positiva con las distancias geográficas. Conclusiones: los resultados son congruentes con el aislamiento histórico de las comunidades y reciente tendencia a la homogenización por las altas tasas de migración masculina intercomunitaria. Las características de la población garífuna la hacen propicia para la realización de estudios destinados a la búsqueda de genes de susceptibilidad relacionados con enfermedades de herencia compleja.

  18. Competition entre supraconductivite et magnetisme au voisinage de la transition de Mott dans le conducteur organique quasi-bidimensionnel k-(BEDT-TTF)2copper[N(CN)2]bromine

    NASA Astrophysics Data System (ADS)

    Fournier, David

    Les conducteurs organiques quasi-bidimensionnels kappa-ET2X presentent d'importantes similitudes avec les SCHT telles qu'une phase isolant de Mott, un regime de pseudogap et un etat supraconducteur. L'etude de leurs proprietes apparait donc complementaire. Parmi les interrogations persistantes concernant la physique de ces systemes, l'origine du (ou des) processus exotique d'appariement, responsable de la supraconductivite est le sujet suscitant l'interet le plus marque dans la communaute. L'hypothese d'un mecanisme lie a la proximite d'un etat antiferromagnetique est privilegiee. Une etape importante dans la resolution de cette problematique est l'identification de la symetrie du parametre d'ordre. D'apres de nombreux travaux sur les systemes fortement correles, la sonde ultrasonore, de par sa sensibilite aux excitations de quasiparticule a basse temperature, est consideree comme particulierement adaptee a l'etude de cette propriete. Cependant, son emploi necessite l'utilisation d'un compose metallique a basse temperature et completement supraconducteur. Le compose metallique organique kappa-ET 2Cu[N(CN)2]Br presente toutes les caracteristiques necessaires a l'etude de cette propriete. En effet, il est situe loin de la transition du premier ordre de Mott et est completement supraconducteur. De facon surprenante, ce systeme semble se coupler fortement avec le reseau ce qui augmente significativement la sensibilite de cette sonde aux proprietes du gaz electronique. Cependant, des difficultes techniques importantes, liees a la nature intrinseque de ce materiau, doivent etre surmontees pour proceder a des mesures suivant differentes polarisations. La presente etude a profondement modifie notre comprehension de ce systeme. En effet, ces mesures ont permis de constater que le kappa-ET2Cu[N(CN)2]Br est un compose qui est situe en bordure de la zone de coexistence entre la supraconductivite et le magnetisme, ce qui constitue un resultat totalement inattendu. De plus, la

  19. Etude théorique de l'interaction de van der Waals entre une sonde métallique et une surface diélectrique : application à la microscopie par mesure de forces atomiques

    NASA Astrophysics Data System (ADS)

    Maghezzi, S.; Girard, C.; van Labeke, D.

    1991-02-01

    The van der Waals interaction force between a metallic tip and a non planar dielectric is derived from a non local formalism. A general formulation is given for a spherical tip of nanometric size and for surfaces of arbitrary shape (monocrystal adsorbed on planar surfaces). The dispersion part of the attractive force is obtained from a non local theory expressed in terms of generalized electric susceptibilities of the two partners. Implications for atomic force microscopy in attractive mode are also discussed. La force d'interaction de van der Waals entre une pointe métallique et une surface diélectrique est calculée à partir d'une théorie non-locale. Plus précisément, on considère comme point de départ que l'interaction sonde-surface est due au couplage entre les fluctuations des densités de charges des solides en présence. De plus, dans cette approche la force de van der Waals est calculée dans le cadre de la théorie de la réponse linéaire en utilisant pour chacun des milieux la fonction réponse non-locale correspondante. En particulier la sonde, de nature métallique, est caractérisée par un jeu de susceptibilités du champ décrites par un formalisme auto-cohérent. On effectue enfin une étude numérique de la force sur des surfaces présentant des corrugations de dimensions nanométriques.

  20. Factores socio-económicos asociados a la percepción de situación socioeconómica entre adultos mayores de dos países latinoamericanos

    PubMed Central

    Brenes-Camacho, Gilbert

    2014-01-01

    El objetivo principal del artículo es estudiar la asociación entre la percepción subjetiva sobre la situación económica propia y una serie de medidas objetivas de bienestar socioeconómico –fuentes de ingresos, tenencia de vivienda, nivel educativo y transferencias familiares informales de dinero- entre adultos mayores de dos países Latinoamericanos: México y Costa Rica. Los datos se obtienen de las primeras rondas de dos encuestas sobre envejecimiento: CRELES para Costa Rica y ENASEM para México. La variable dependiente más importante se obtiene de las respuestas a las pregunta “¿Cómo califica su situación económica actual?” en Costa Rica y “¿Diría usted que su situación económica es…?” en México. Para ambas encuestas, las respuestas se codificaron en forma binaria; el código 0 representa las categorías Excelente, Muy buena y Buena, y el código 1 representa a las categorías Regular y Mala. Se encontró que el ingreso por jubilación es un importante determinante de la percepción de bienestar en ambos países. En Costa Rica, el ingreso del cónyuge y la tenencia de vivienda son importantes predictores de la percepción de bienestar, mientras que en México, los ingresos por transferencias están fuertemente asociados con dicha percepción. PMID:25360057

  1. Aceptabilidad del diagnóstico rápido casero para HIV entre hombres gay y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires.

    PubMed

    Balán, Iván C; Carballo-Diéguez, Alex; Marone, Rubén O; Pando, María A; Barreda, Victoria; Avila, María M

    2011-03-01

    El uso del diagnóstico rápido para HIV en Argentina, así como otros países de Latinoamérica, ha sido limitado hasta el momento. Este trabajo reporta los resultados provenientes de un estudio cualitativo realizado entre hombres gays y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires, Argentina. El objetivo principal del mismo fue conocer las ventajas y desventajas que los hombres G&HSH perciben en relación al diagnóstico rápido casero para HIV. Se realizaron ocho grupos focales con 73 participantes en los cuales se discutió acerca de las ventajas y desventajas del uso de los diagnósticos rápidos. Las respuestas fueron codificadas utilizando un programa para análisis de datos cualitativos (NVivo) y analizadas temáticamente. Los participantes describieron numerosas ventajas sobre el uso del diagnóstico rápido casero, aunque algunos reportaron importantes preocupaciones dentro de las cuales se destaca la posibilidad de impulsos suicidas si alguien recibe un resultado positivo estando solo. En términos generales se observó una gran aceptabilidad para el uso del diagnóstico rápido si el mismo es realizado por personal de salud en lugares acondicionados para este fin.

  2. Determinação de elementos próprios dos asteróides troianos: comparação entre as teorias semi-analítica e sintética

    NASA Astrophysics Data System (ADS)

    Roig, F.; Beaugé, C.

    2003-08-01

    Além do cálculo semi-analítico de elementos próprios dos asteróides Troianos (Beaugé & Roig 2001, Icarus 153, 391), recentemente foi apresentado um novo conjunto destes elementos próprios determinado através de uma teoria sintética (Knenezevic & Milani 2003, comunicação pessoal). As bases de dados contendo estas determinações estão disponiveis na pagina web do Asteroid Dynamical Site (http://hamilton.dm.unipi.it/cgi-bin/astdys/astibo). Nesta comunicação apresentamos os primeiros resultados de um estudo comparativo entre ambos conjuntos de elementos próprios, analisando suas vantagens e desvantagens, assim como os limites de precisão de cada conjunto. Mostramos que os elementos próprios sintéticos são mais precisos que os smi-analíticos para grandes amplitudes de libração do ângulo s = l-lJup, embora acontece o contrario para os corpos cuja amplitude de libração é muito pequena. Finalmente discutimos a influencia destes erros na determinação de familias de asteroides e da estrutura resonante em torno dos pontos Lagrangeanos L4 e L5.

  3. Aceptabilidad del diagnóstico rápido casero para HIV entre hombres gay y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires

    PubMed Central

    Balán, Iván C.; Carballo-Diéguez, Alex; Marone, Rubén O.; Pando, María A.; Barreda, Victoria; Ávila, María M.

    2011-01-01

    Resumen El uso del diagnóstico rápido para HIV en Argentina, así como otros países de Latinoamérica, ha sido limitado hasta el momento. Este trabajo reporta los resultados provenientes de un estudio cualitativo realizado entre hombres gays y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires, Argentina. El objetivo principal del mismo fue conocer las ventajas y desventajas que los hombres G&HSH perciben en relación al diagnóstico rápido casero para HIV. Se realizaron ocho grupos focales con 73 participantes en los cuales se discutió acerca de las ventajas y desventajas del uso de los diagnósticos rápidos. Las respuestas fueron codificadas utilizando un programa para análisis de datos cualitativos (NVivo) y analizadas temáticamente. Los participantes describieron numerosas ventajas sobre el uso del diagnóstico rápido casero, aunque algunos reportaron importantes preocupaciones dentro de las cuales se destaca la posibilidad de impulsos suicidas si alguien recibe un resultado positivo estando solo. En términos generales se observó una gran aceptabilidad para el uso del diagnóstico rápido si el mismo es realizado por personal de salud en lugares acondicionados para este fin. PMID:25284951

  4. Traces de l'interaction entre galaxies

    NASA Astrophysics Data System (ADS)

    Duc, Pierre-Alain

    2016-08-01

    Within a galaxy, collisions between stars are exceptional; collisions between galaxies are themselves much more frequent. They are even supposed to play a major role in the formation of structures according to the standard hierarchical cosmological model. Gravitational interactions, tidal forces and following mergers shape the morphology of galaxies, and leave vestiges which can survive for a few Gyr. They consist of stellar shells, streams, tails and plumes which emit a diffuse and extended optical light. Several deep imaging projects use telescopes of all sizes to try to detect this light. We detail here what the census of collisional debris can tel us about the past history of galaxies and about the models and simulations supposedly accounting for it.

  5. Étude physico-chimique des réactions entre oxydes d'azote Application à l'analyse de ces gaz en sortie de systèmes de combustion

    NASA Astrophysics Data System (ADS)

    Leblanc, E.; Jokela, K.; Perier-Camby, L.; Thomas, G.

    1999-05-01

    éaction pour des paramètres expérimentaux représentatifs des atmosphères générées par les systèmes de combustion (teneur en NOx : 1000 ppm, présence d'oxygène, température comprise entre 25 circC et 1000 circC). Les constantes cinétiques ont ainsi été déterminées pour des températures inférieures à 300 circC. L'influence catalytique de la paroi du réacteur a été mise en évidence au delà de cette température. Les valeurs concernant les équilibres thermodynamiques entre les NOx ont également pu être validées expérimentalement.

  6. Distinción Empírica Entre Engagement y Trabajolismo en Enfermeras Hospitalarias de Japón: Efecto Sobre la Calidad del Sueño y el Desempeño Laboral

    PubMed Central

    Kubota, Kazumi; Shimazu, Akihito; Kawakami, Norito; Takahashi, Masaya; Nakata, Akinori; Schaufeli, Wilmar B.

    2016-01-01

    Objetivo El objetivo de este estudio es demostrar la distinción entre engagement y trabajolismo, estudiando su relación con la calidad del sueño y el desempeño laboral. Método Un total de 447 enfermeras de 3 hospitales de Japón fueron entrevistadas mediante un cuestionario autoadministrado que incluía la escala Utrecht (UWES, Utrecht Work Engagement Scale), la Escala de Adicción al Trabajo Holandesa (DUWAS, Dutch Workaholism Scale), preguntas sobre la calidad del sueño (7 ítems) con respecto a (1) dificultad para conciliar el sueño, (2) dificultad para mantener el sueño, (3) despertar temprano por la mañana, (4) dormirse o tomar siestas durante el día, (5) somnolencia diurna excesiva en el trabajo, (6) dificultad para despertarse por la mañana, y (7) despertar cansado en la mañana, y el Cuestionario sobre Salud y Desempeño (CSD) de la Organización Mundial de la Salud. Resultados Los modelos de ecuaciones estructurales demostraron que el engagement se relaciona positivamente con la calidad del sueño y el rendimiento laboral, mientras que el trabajolismo tiene una relación negativa con la calidad del sueño y el desempeño laboral. Conclusión Los resultados indican que el engagement y el trabajolismo son conceptualmente diferentes. El primero tiene una connotación positiva, mientras que el segundo se asocia de manera negativa al bienestar (buena calidad del sueño y buen rendimiento en el trabajo). PMID:26752805

  7. L’annonce d’une déficience motrice cérébrale : Une relation de confiance à construire entre les parents, le personnel paramédical et les médecins

    PubMed Central

    Pelchat, Diane; Lefebvre, Hélène; Bouchard, Jean-Marie

    2001-01-01

    RÉSUMÉ : L’annonce d’une déficience motrice cérébrale et des incapacités qui en découlent est une situation difficile à vivre pour les parents et les dispensateurs de soins (personnel paramédical et médecins). Un examen de la documentation médicale démontre que depuis vingt ans, le nombre de parents insatisfaits n’a pas changé. L’étude de type qualitatif et exploratoire, effectuée à la demande de milieux de réadaptation, vise à décrire le processus d’annonce d’un diagnostic de déficience motrice cérébrale (DMC) à partir de l’expérience des différents acteurs en cause et à déterminer les facteurs qui facilitent ou compliquent la relation de confiance. Les résultats indiquent que les parents et les dispensateurs de soins vivent dans deux mondes de perception différents au moment de l’annonce, lesquels se rejoignent difficilement. Les parents sont envahis par le drame qu’ils vivent, tandis que les médecins sont concentrés sur le contenu à transmettre aux parents. Cette annonce provoque une importante incertitude qui bloque la communication entre les partenaires, mais elle pourrait devenir un stimulant à de nouvelles stratégies d’adaptation. PMID:20084263

  8. Pollution Atmosphérique et Santé: Corrélation ou Causalité? Le Cas de la Relation entre l'Exposition aux Particules et la Mortalité Cardio-pulmonaire.

    PubMed

    Dab, William; Ségala, Claire; Dor, Frédéric; Festy, Bernard; Lameloise, Philippe; Moullec, Yvon Le; Tertre, Alain Le; Médina, Sylvia; Quénel, Philippe; Wallaert, Benoît; Zmirou, Et Denis

    2001-02-01

    De nombreuses études épidémiologiques ont observé dans des contextes différents une faible relation à court terme entre les particules et la mortalité cardio-pulmonaire, même quand les normes de qualité de l'air n'étaient pas dépassées. La causalité de cette relation est un enjeu de santé publique en raison de l'importance de la population exposée. Notre objectif est de faire l'inventaire critique des arguments utilisés dans 15 revues de la littérature publiées. Nous expliquons l'importance de distinguer la validité de la causalité et analysons de façon systématique les différents critères de jugement dans le contexte des études écologiques temporelles. Notre conclusion est que la relation observée est valide et que la plupart des critères de causalité sont respectés. Diminuer le niveau d'exposition des populations aux particules est souhaitable. En Europe, en agissant à la source, notamment sur les émissions Diesel, on diminuera aussi d'autres polluants qui peuvent jouer un rôle sanitaire. Aux États-Unis, la situation est plus complexe car les particules sont surtout secondaires. Il est également indispensable de poursuivre les recherches pour mieux connaître les déterminants des expositions globales des individus et mieux comprendre le rôle toxique des différents facteurs physico-chimiques des particules.

  9. Stress-Mediated cis-Element Transcription Factor Interactions Interconnecting Primary and Specialized Metabolism in planta

    PubMed Central

    Sheshadri, S. A.; Nishanth, M. J.; Simon, Bindu

    2016-01-01

    Plant specialized metabolites are being used worldwide as therapeutic agents against several diseases. Since the precursors for specialized metabolites come through primary metabolism, extensive investigations have been carried out to understand the detailed connection between primary and specialized metabolism at various levels. Stress regulates the expression of primary and specialized metabolism genes at the transcriptional level via transcription factors binding to specific cis-elements. The presence of varied cis-element signatures upstream to different stress-responsive genes and their transcription factor binding patterns provide a prospective molecular link among diverse metabolic pathways. The pattern of occurrence of these cis-elements (overrepresentation/common) decipher the mechanism of stress-responsive upregulation of downstream genes, simultaneously forming a molecular bridge between primary and specialized metabolisms. Though many studies have been conducted on the transcriptional regulation of stress-mediated primary or specialized metabolism genes, but not much data is available with regard to cis-element signatures and transcription factors that simultaneously modulate both pathway genes. Hence, our major focus would be to present a comprehensive analysis of the stress-mediated interconnection between primary and specialized metabolism genes via the interaction between different transcription factors and their corresponding cis-elements. In future, this study could be further utilized for the overexpression of the specific transcription factors that upregulate both primary and specialized metabolism, thereby simultaneously improving the yield and therapeutic content of plants. PMID:27933071

  10. A DNA-Based Procedure for In Planta Detection of Fusarium oxysporum f. sp. phaseoli.

    PubMed

    Alves-Santos, Fernando M; Ramos, Brisa; García-Sánchez, M Asunción; Eslava, Arturo P; Díaz-Mínguez, José María

    2002-03-01

    ABSTRACT We have characterized strains of Fusarium oxysporum from common bean fields in Spain that were nonpathogenic on common bean, as well as F. oxysporum strains (F. oxysporum f. sp. phaseoli) pathogenic to common bean by random amplified polymorphic DNA (RAPD) analysis. We identified a RAPD marker (RAPD 4.12) specific for the highly virulent pathogenic strains of the seven races of F. oxysporum f. sp. phaseoli. Sequence analysis of RAPD 4.12 allowed the design of oligonucleotides that amplify a 609-bp sequence characterized amplified region (SCAR) marker (SCAR-B310A280). Under controlled environmental and greenhouse conditions, detection of the pathogen by polymerase chain reaction was 100% successful in root samples of infected but still symptomless plants and in stem samples of plants with disease severity of >/=4 in the Centro Internacional de Agricultura Tropical (CIAT; Cali, Colombia) scale. The diagnostic procedure can be completed in 5 h and allows the detection of all known races of the pathogen in plant samples at early stages of the disease with no visible symptoms.

  11. Origin and evolution of metal P-type ATPases in Plantae (Archaeplastida)

    PubMed Central

    Hanikenne, Marc; Baurain, Denis

    2013-01-01

    Metal ATPases are a subfamily of P-type ATPases involved in the transport of metal cations across biological membranes. They all share an architecture featuring eight transmembrane domains in pairs of two and are found in prokaryotes as well as in a variety of Eukaryotes. In Arabidopsis thaliana, eight metal P-type ATPases have been described, four being specific to copper transport and four displaying a broader metal specificity, including zinc, cadmium, and possibly copper and calcium. So far, few efforts have been devoted to elucidating the origin and evolution of these proteins in Eukaryotes. In this work, we use large-scale phylogenetics to show that metal P-type ATPases form a homogenous group among P-type ATPases and that their specialization into either monovalent (Cu) or divalent (Zn, Cd…) metal transport stems from a gene duplication that took place early in the evolution of Life. Then, we demonstrate that the four subgroups of plant metal ATPases all have a different evolutionary origin and a specific taxonomic distribution, only one tracing back to the cyanobacterial progenitor of the chloroplast. Finally, we examine the subsequent evolution of these proteins in green plants and conclude that the genes thoroughly characterized in model organisms are often the result of lineage-specific gene duplications, which calls for caution when attempting to infer function from sequence similarity alone in non-model organisms. PMID:24575101

  12. Staphylococcal abscess caused by trauma with a rosebush aculeus (Plantae, Rosaceae): apropos of a case.

    PubMed

    Bernardes Filho, Fred; Alves, Andreia Oliveira; Martins, Gustavo; Sasso, Letícia Soares; Gama, Carolina Mendonça; Cenci, Gardênia Borges

    2013-01-01

    Cutaneous abscess is a localized collection of pus in the dermis and subcutaneous tissue usually caused by trauma. The authors report the case of a 30-year-old male patient, gardener, that presents an ulcerated plaque in the third right finger, caused by an aculeus plant wound. The examination of the lesion's exudate ruled out the existence of fungi and showed the presence of Staphylococcus aureus. The authors emphasize the sporotrichosis as an important differential diagnosis, especially in endemic areas, and the indication of the exudate culture is discussed.

  13. Chemical compounds effective against the citrus huanglongbing bacterium 'Candidatus Liberibacter asiaticus' in planta

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Citrus huanglongbing (HLB) is one of the most destructive diseases of citrus worldwide, and is threatening the survival of the Floridian citrus industry. Currently, there is no established cure for this century-old and emerging disease. The new antibiotic combination of penicillin and streptomycin (...

  14. Pharma-Planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals.

    PubMed

    Sparrow, Penelope Amelia Claire; Irwin, Judith A; Dale, Phil J; Twyman, Richard M; Ma, Julian K C

    2007-04-01

    Significant advances over the last few years have seen plant-made pharmaceuticals (PMPs) move from the exploratory research phase towards clinical trials, with the first commercial products for human use expected to reach the market by 2009. Europe has yet to witness the commercial application of PMP technology, although at least one product has begun phase II clinical trials with others following close behind. These emerging products are set to challenge the complex and overlapping regulations that currently govern GM plants and 'conventional' pharmaceutical production. The areas of responsibility are being mapped out between the different EU regulatory agencies, with specific guidelines currently being drawn up for the regulation of PMPs. This article discusses issues surrounding the development of robust risk-assessment and risk-management practices based on health and environmental impact, while working with EU regulatory authorities to ensure appropriate regulatory oversight.

  15. Floral Benzenoid Carboxyl Methyltransferases: From in Vitro to in Planta Function

    SciTech Connect

    Effmert,U.; Saschenbrecker, S.; Ross, J.; Negre, F.; Fraser, C.; Noel, J.; Dudareva, N.; Piechulla, B.

    2005-01-01

    Benzenoid carboxyl methyltransferases synthesize methyl esters (e.g., methyl benzoate and methyl salicylate), which are constituents of aromas and scents of many plant species and play important roles in plant communication with the surrounding environment. Within the past five years, eleven such carboxyl methyltransferases were isolated and most of them were comprehensively investigated at the biochemical, molecular and structural level. Two types of enzymes can be distinguished according to their substrate preferences: the SAMT-type enzymes isolated from Clarkia breweri, Stephanotis floribunda, Antirrhinum majus, Hoya carnosa, and Petunia hybrida, which have a higher catalytic efficiency and preference for salicylic acid, while BAMT-type enzymes from A. majus, Arabidopsis thaliana, Arabidopsis lyrata, and Nicotiana suaveolens prefer benzoic acid. The elucidation of C. breweri SAMT's three-dimensional structure allowed a detailed modelling of the active sites of the carboxyl methyltransferases and revealed that the SAM binding pocket is highly conserved among these enzymes while the methyl acceptor binding site exhibits some variability, allowing a classification into SAMT-type and BAMT-type enzymes. The analysis of expression patterns coupled with biochemical characterization showed that these carboxyl methyltransferases are involved either in floral scent biosynthesis or in plant defense responses. While the latter can be induced by biotic or abiotic stress, the genes responsible for floral scent synthesis exhibit developmental and rhythmic expression pattern. The nature of the product and efficiency of its formation in plants depend on the availability of substrates, the catalytic efficiency of the enzyme toward benzoic acid and/or salicylic acid, and the transcriptional, translational, and post-translational regulation at the enzyme level. The biochemical properties of benzenoid carboxyl methyltransferases suggest that the genes involved in plant defenses might represent the ancestor for the presently existing floral genes which during evolution gained different expression profiles and encoded enzymes with the ability to accept structurally similar substrates.

  16. In-Planta Expression: Searching for the Genuine Chromophores of Cryptochrome-3 from Arabidopsis thaliana.

    PubMed

    Gärtner, Wolfgang

    2017-01-01

    Göbel et al. present in this issue an exemplary study of identification of chromophores from Arabidopsis thaliana cryptochrome-3. Usually taken for granted, proteins and cofactors, respective chromophores, from heterologous expression are considered identical to material isolated from their genuine host. Cryptochromes carry two chromophores, an antenna cofactor and a functional flavin chromophore, both noncovalently embedded into the protein. In particular the antenna chromophore is loosely bound and often lost during protein purification. The authors identify from plant-extracted Cry3 unambiguously N(5) ,N(10) -methenyltetrahydrofolate as antenna chromophore and flavin adenine dinucleotide as the functional chromophore.

  17. Comparative assessments of CRISPR-Cas nucleases' cleavage efficiency in planta.

    PubMed

    Johnson, Ross A; Gurevich, Vyacheslav; Filler, Shdema; Samach, Aviva; Levy, Avraham A

    2015-01-01

    Custom-designed nucleases can enable precise plant genome editing by catalyzing DNA-breakage at specific targets to stimulate targeted mutagenesis or gene replacement. The CRISPR-Cas system, with its target-specifying RNA molecule to direct the Cas9 nuclease, is a recent addition to existing nucleases that bind and cleave the target through linked protein domains (e.g. TALENs and zinc-finger nucleases). We have conducted a comparative study of these different types of custom-designed nucleases and we have assessed various components of the CRISPR-Cas system. For this purpose, we have adapted our previously reported assay for cleavage-dependent luciferase gene correction in Nicotiana benthamiana leaves (Johnson et al. in Plant Mol Biol 82(3):207-221, 2013). We found that cleavage by CRISPR-Cas was more efficient than cleavage of the same target by TALENs. We also compared the cleavage efficiency of the Streptococcus pyogenes Cas9 protein based on expression using three different Cas9 gene variants. We found significant differences in cleavage efficiency between these variants, with human and Arabidopsis thaliana codon-optimized genes having the highest cleavage efficiencies. We compared the activity of 12 de novo-designed single synthetic guide RNA (sgRNA) constructs, and found their cleavage efficiency varied drastically when using the same Cas9 nuclease. Finally, we show that, for one of the targets tested with our assay, we could induce a germinally-transmitted deletion in a repeat array in A. thaliana. This work emphasizes the efficiency of the CRISPR-Cas system in plants. It also shows that further work is needed to be able to predict the optimal design of sgRNAs or Cas9 variants.

  18. The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods

    PubMed Central

    Bhat, Riyaz A; Lahaye, Thomas; Panstruga, Ralph

    2006-01-01

    Non-invasive fluorophore-based protein interaction assays like fluorescence resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC, also referred to as "split YFP") have been proven invaluable tools to study protein-protein interactions in living cells. Both methods are now frequently used in the plant sciences and are likely to develop into standard techniques for the identification, verification and in-depth analysis of polypeptide interactions. In this review, we address the individual strengths and weaknesses of both approaches and provide an outlook about new directions and possible future developments for both techniques. PMID:16800872

  19. Chrysanthemyl Diphosphate Synthase Operates in Planta as a Bifunctional Enzyme with Chrysanthemol Synthase Activity*

    PubMed Central

    Yang, Ting; Gao, Liping; Hu, Hao; Stoopen, Geert; Wang, Caiyun; Jongsma, Maarten A.

    2014-01-01

    Chrysanthemyl diphosphate synthase (CDS) is the first pathway-specific enzyme in the biosynthesis of pyrethrins, the most widely used plant-derived pesticide. CDS catalyzes c1′-2-3 cyclopropanation reactions of two molecules of dimethylallyl diphosphate (DMAPP) to yield chrysanthemyl diphosphate (CPP). Three proteins are known to catalyze this cyclopropanation reaction of terpene precursors. Two of them, phytoene and squalene synthase, are bifunctional enzymes with both prenyltransferase and terpene synthase activity. CDS, the other member, has been reported to perform only the prenyltransferase step. Here we show that the NDXXD catalytic motif of CDS, under the lower substrate conditions prevalent in plants, also catalyzes the next step, converting CPP into chrysanthemol by hydrolyzing the diphosphate moiety. The enzymatic hydrolysis reaction followed conventional Michaelis-Menten kinetics, with a Km value for CPP of 196 μm. For the chrysanthemol synthase activity, DMAPP competed with CPP as substrate. The DMAPP concentration required for half-maximal activity to produce chrysanthemol was ∼100 μm, and significant substrate inhibition was observed at elevated DMAPP concentrations. The N-terminal peptide of CDS was identified as a plastid-targeting peptide. Transgenic tobacco plants overexpressing CDS emitted chrysanthemol at a rate of 0.12–0.16 μg h−1 g−1 fresh weight. We propose that CDS should be renamed a chrysanthemol synthase utilizing DMAPP as substrate. PMID:25378387

  20. The potential of nuclear magnetic resonance to track lipids in planta.

    PubMed

    Munz, Eberhard; Jakob, Peter M; Borisjuk, Ljudmilla

    2016-11-01

    Nuclear Magnetic Resonance (NMR) provides a highly flexible platform for non invasive analysis and imaging biological samples, since the manipulation of nuclear spin allows the tailoring of experiments to maximize the informativeness of the data. MRI is capable of visualizing a holistic picture of the lipid storage in living plant/seed. This review has sought to explain how the technology can be used to acquire functional and physiological data from plant samples, and how to exploit it to characterize lipid deposition in vivo. At the same time, we have referred to the current limitations of NMR technology as applied to plants, and in particular of the difficulty of transferring methodologies optimized for animal/medical subjects to plant ones. A forward look into likely developments in the field is included, anticipating its key future role in the study of living plant.

  1. Angiogenic activity of latex from Euphorbia tirucalli Linnaeus 1753 (Plantae, Euphorbiaceae).

    PubMed

    Bessa, G; Melo-Reis, P R; Araújo, L A; Mrué, F; Freitas, G B; Brandão, M L; Silva Júnior, N J

    2015-08-01

    To assess the pro-angiogenic activity of Euphorbia tirucalli, commonly known as "avelós" plant, we performed a series of tests by applying an aqueous E. tirucalli latex solution (10 mg/mL) to the chorioallantoic membranes (CAMs) of 80 fertilized chicken eggs incubated in a temperature- and humidity-controlled automatic incubator. The results indicated that the aqueous latex solution increased vascular network formation compared to that with the negative control (p < 0.05) and the inhibitor control (p < 0.05). This suggests that under the experimental conditions tested, the aqueous latex solution induced an inflammatory response leading to neoangiogenesis.

  2. Quantification of Sclerotinia homoeocarpa overwintering in planta and detection in commercial seed

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Dollar spot is the most economically important disease of amenity turf grasses in the United States, yet little is known about the source of primary inoculum for this disease. With the exception of a few isolates from the United Kingdom, Sclerotinia homoeocarpa, the causal agent of dollar spot, does...

  3. Wide screening of phage-displayed libraries identifies immune targets in planta.

    PubMed

    Rioja, Cristina; Van Wees, Saskia C; Charlton, Keith A; Pieterse, Corné M J; Lorenzo, Oscar; García-Sánchez, Susana

    2013-01-01

    Microbe-Associated Molecular Patterns and virulence effectors are recognized by plants as a first step to mount a defence response against potential pathogens. This recognition involves a large family of extracellular membrane receptors and other immune proteins located in different sub-cellular compartments. We have used phage-display technology to express and select for Arabidopsis proteins able to bind bacterial pathogens. To rapidly identify microbe-bound phage, we developed a monitoring method based on microarrays. This combined strategy allowed for a genome-wide screening of plant proteins involved in pathogen perception. Two phage libraries for high-throughput selection were constructed from cDNA of plants infected with Pseudomonas aeruginosa PA14, or from combined samples of the virulent isolate DC3000 of Pseudomonas syringae pv. tomato and its avirulent variant avrRpt2. These three pathosystems represent different degrees in the specificity of plant-microbe interactions. Libraries cover up to 2 × 10(7) different plant transcripts that can be displayed as functional proteins on the surface of T7 bacteriophage. A number of these were selected in a bio-panning assay for binding to Pseudomonas cells. Among the selected clones we isolated the ethylene response factor ATERF-1, which was able to bind the three bacterial strains in competition assays. ATERF-1 was rapidly exported from the nucleus upon infiltration of either alive or heat-killed Pseudomonas. Moreover, aterf-1 mutants exhibited enhanced susceptibility to infection. These findings suggest that ATERF-1 contains a microbe-recognition domain with a role in plant defence. To identify other putative pathogen-binding proteins on a genome-wide scale, the copy number of selected-vs.-total clones was compared by hybridizing phage cDNAs with Arabidopsis microarrays. Microarray analysis revealed a set of 472 candidates with significant fold change. Within this set defence-related genes, including well-known targets of bacterial effectors, are over-represented. Other genes non-previously related to defence can be associated through this study with general or strain-specific recognition of Pseudomonas.

  4. In planta localisation patterns of MADS domain proteins during floral development in Arabidopsis thaliana

    PubMed Central

    Urbanus, Susan L; de Folter, Stefan; Shchennikova, Anna V; Kaufmann, Kerstin; Immink, Richard GH; Angenent, Gerco C

    2009-01-01

    Background MADS domain transcription factors play important roles in various developmental processes in flowering plants. Members of this family play a prominent role in the transition to flowering and the specification of floral organ identity. Several studies reported mRNA expression patterns of the genes encoding these MADS domain proteins, however, these studies do not provide the necessary information on the temporal and spatial localisation of the proteins. We have made GREEN FLUORESCENT PROTEIN (GFP) translational fusions with the four MADS domain proteins SEPALLATA3, AGAMOUS, FRUITFULL and APETALA1 from the model plant Arabidopsis thaliana and analysed the protein localisation patterns in living plant tissues by confocal laser scanning microscopy (CLSM). Results We unravelled the protein localisation patterns of the four MADS domain proteins at a cellular and subcellular level in inflorescence and floral meristems, during development of the early flower bud stages, and during further differentiation of the floral organs. The protein localisation patterns revealed a few deviations from known mRNA expression patterns, suggesting a non-cell autonomous action of these factors or alternative control mechanisms. In addition, we observed a change in the subcellular localisation of SEPALLATA3 from a predominantly nuclear localisation to a more cytoplasmic localisation, occurring specifically during petal and stamen development. Furthermore, we show that the down-regulation of the homeodomain transcription factor WUSCHEL in ovular tissues is preceded by the occurrence of both AGAMOUS and SEPALLATA3 proteins, supporting the hypothesis that both proteins together suppress WUSCHEL expression in the ovule. Conclusion This approach provides a highly detailed in situ map of MADS domain protein presence during early and later stages of floral development. The subcellular localisation of the transcription factors in the cytoplasm, as observed at certain stages during development, points to mechanisms other than transcriptional control. Together this information is essential to understand the role of these proteins in the regulatory processes that drive floral development and leads to new hypotheses. PMID:19138429

  5. Complete genome sequence and in planta subcellular localization of maize fine streak virus proteins.

    PubMed

    Tsai, Chi-Wei; Redinbaugh, Margaret G; Willie, Kristen J; Reed, Sharon; Goodin, Michael; Hogenhout, Saskia A

    2005-05-01

    The genome of the nucleorhabdovirus maize fine streak virus (MFSV) consists of 13,782 nucleotides of nonsegmented, negative-sense, single-stranded RNA. The antigenomic strand consisted of seven open reading frames (ORFs), and transcripts of all ORFs were detected in infected plants. ORF1, ORF6, and ORF7 had significant similarities to the nucleocapsid protein (N), glycoprotein (G), and polymerase (L) genes of other rhabdoviruses, respectively, whereas the ORF2, ORF3, ORF4, and ORF5 proteins had no significant similarities. The N (ORF1), ORF4, and ORF5 proteins localized to nuclei, consistent with the presence of nuclear localization signals (NLSs) in these proteins. ORF5 likely encodes the matrix protein (M), based on its size, the position of its NLS, and the localization of fluorescent protein fusions to the nucleus. ORF2 probably encodes the phosphoprotein (P) because, like the P protein of Sonchus yellow net virus (SYNV), it was spread throughout the cell when expressed alone but was relocalized to a subnuclear locus when coexpressed with the MFSV N protein. Unexpectedly, coexpression of the MFSV N and P proteins, but not the orthologous proteins of SYNV, resulted in accumulations of both proteins in the nucleolus. The N and P protein relocalization was specific to cognate proteins of each virus. The subcellular localizations of the MFSV ORF3 and ORF4 proteins were distinct from that of the SYNV sc4 protein, suggesting different functions. To our knowledge, this is the first comparative study of the cellular localizations of plant rhabdoviral proteins. This study indicated that plant rhabdoviruses are diverse in genome sequence and viral protein interactions.

  6. "In planta" regulation of the "Arabidopsis" Ca(2+)/H(+) antiporter CAX1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vacuolar localized Ca(2+)/H(+) exchangers such as "Arabidopsis thaliana" cation exchanger 1 (CAX1) play important roles in Ca(2+) homeostasis. When expressed in yeast, CAX1 is regulated via an N-terminal autoinhibitory domain. In yeast expression assays, a 36 amino acid N-terminal truncation of CAX1...

  7. Potyviral VPg enhances viral RNA Translation and inhibits reporter mRNA translation in planta.

    PubMed

    Eskelin, Katri; Hafrén, Anders; Rantalainen, Kimmo I; Mäkinen, Kristiina

    2011-09-01

    Viral protein genome-linked (VPg) plays a central role in several stages of potyvirus infection. This study sought to answer questions about the role of Potato virus A (PVA; genus Potyvirus) VPg in viral and host RNA expression. When expressed in Nicotiana benthamiana leaves in trans, a dual role of VPg in translation is observed. It repressed the expression of monocistronic luciferase (luc) mRNA and simultaneously induced a significant upregulation in the expression of both replicating and nonreplicating PVA RNAs. This enhanced viral gene expression was due at least to the 5' untranslated region (UTR) of PVA RNA, eukaryotic initiation factors 4E and iso 4E [eIF4E/eIF(iso)4E], and the presence of a sufficient amount of VPg. Coexpression of VPg with viral RNA increased the viral RNA amount, which was not the case with the monocistronic mRNA. Both mutations at certain lysine residues in PVA VPg and eIF4E/eIF(iso)4E depletion reduced its ability to upregulate the viral RNA expression. These modifications were also involved in VPg-mediated downregulation of monocistronic luc expression. These results suggest that VPg can titrate eIF4Es from capped monocistronic RNAs. Because VPg-mediated enhancement of viral gene expression required eIF4Es, it is possible that VPg directs eIF4Es to promote viral RNA expression. From this study it is evident that VPg can serve as a specific regulator of PVA expression by boosting the viral RNA amounts as well as the accumulation of viral translation products. Such a mechanism could function to protect viral RNA from being degraded and to secure efficient production of coat protein (CP) for virion formation.

  8. In planta reduction of maize seedling stalk lesions by the bacterial endophyte Bacillus mojavensis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Due to autoinfection and alloinfection, maize is susceptible to infection by Fusarium verticillioides resulting in diseases and contamination of maize kernels with the fumonisin mycotoxins. Attempts at controlling this fungus are currently being done with biocontrol agents such as bacteria and this...

  9. Auxin transport sites are visualized in planta using fluorescent auxin analogs.

    PubMed

    Hayashi, Ken-ichiro; Nakamura, Shouichi; Fukunaga, Shiho; Nishimura, Takeshi; Jenness, Mark K; Murphy, Angus S; Motose, Hiroyasu; Nozaki, Hiroshi; Furutani, Masahiko; Aoyama, Takashi

    2014-08-05

    The plant hormone auxin is a key morphogenetic signal that controls many aspects of plant growth and development. Cellular auxin levels are coordinately regulated by multiple processes, including auxin biosynthesis and the polar transport and metabolic pathways. The auxin concentration gradient determines plant organ positioning and growth responses to environmental cues. Auxin transport systems play crucial roles in the spatiotemporal regulation of the auxin gradient. This auxin gradient has been analyzed using SCF-type E3 ubiquitin-ligase complex-based auxin biosensors in synthetic auxin-responsive reporter lines. However, the contributions of auxin biosynthesis and metabolism to the auxin gradient have been largely elusive. Additionally, the available information on subcellular auxin localization is still limited. Here we designed fluorescently labeled auxin analogs that remain active for auxin transport but are inactive for auxin signaling and metabolism. Fluorescent auxin analogs enable the selective visualization of the distribution of auxin by the auxin transport system. Together with auxin biosynthesis inhibitors and an auxin biosensor, these analogs indicated a substantial contribution of local auxin biosynthesis to the formation of auxin maxima at the root apex. Moreover, fluorescent auxin analogs mainly localized to the endoplasmic reticulum in cultured cells and roots, implying the presence of a subcellular auxin gradient in the cells. Our work not only provides a useful tool for the plant chemical biology field but also demonstrates a new strategy for imaging the distribution of small-molecule hormones.

  10. Chemical compounds effective against the citrus Huanglongbing bacterium 'Candidatus Liberibacter asiaticus' in planta.

    PubMed

    Zhang, Muqing; Powell, Charles A; Zhou, Lijuan; He, Zhenli; Stover, Ed; Duan, Yongping

    2011-09-01

    Citrus Huanglongbing (HLB) is one of the most destructive diseases of citrus worldwide and is threatening the survival of the Floridian citrus industry. Currently, there is no established cure for this century-old and emerging disease. As a possible control strategy for citrus HLB, therapeutic compounds were screened using a propagation test system with 'Candidatus Liberibacter asiaticus'-infected periwinkle and citrus plants. The results demonstrated that the combination of penicillin and streptomycin (PS) was effective in eliminating or suppressing the 'Ca. L. asiaticus' bacterium and provided a therapeutically effective level of control for a much longer period of time than when administering either antibiotic separately. When treated with the PS, 'Ca. L. asiaticus'-infected periwinkle cuttings achieved 70% of regeneration rates versus <50% by other treatments. The 'Ca. L. asiaticus' bacterial titers in the infected periwinkle plants, as measured by quantitative real-time polymerase chain reaction, decreased significantly following root soaking or foliar spraying with PS. Application of the PS via trunk injection or root soaking also eliminated or suppressed the 'Ca. L. asiaticus' bacterium in the HLB-affected citrus plants. This may provide a useful tool for the management of citrus HLB and other Liberibacter-associated diseases.

  11. Stable plasmid vectors for complementation of Xylella fastidiosa mutants in planta

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Current understanding of the mechanisms of Pierce’s Disease development has been significantly advanced by molecular genetic studies of the causal agent, Xylella fastidiosa (Xf). Plasmid vectors are an essential tool for studies of bacterial genetics and pathogenesis. However, most commonly used pla...

  12. A gateway cloning vector set for high-throughput functional analysis of genes in planta.

    PubMed

    Curtis, Mark D; Grossniklaus, Ueli

    2003-10-01

    The current challenge, now that two plant genomes have been sequenced, is to assign a function to the increasing number of predicted genes. In Arabidopsis, approximately 55% of genes can be assigned a putative function, however, less than 8% of these have been assigned a function by direct experimental evidence. To identify these functions, many genes will have to undergo comprehensive analyses, which will include the production of chimeric transgenes for constitutive or inducible ectopic expression, for antisense or dominant negative expression, for subcellular localization studies, for promoter analysis, and for gene complementation studies. The production of such transgenes is often hampered by laborious conventional cloning technology that relies on restriction digestion and ligation. With the aim of providing tools for high throughput gene analysis, we have produced a Gateway-compatible Agrobacterium sp. binary vector system that facilitates fast and reliable DNA cloning. This collection of vectors is freely available, for noncommercial purposes, and can be used for the ectopic expression of genes either constitutively or inducibly. The vectors can be used for the expression of protein fusions to the Aequorea victoria green fluorescent protein and to the beta-glucuronidase protein so that the subcellular localization of a protein can be identified. They can also be used to generate promoter-reporter constructs and to facilitate efficient cloning of genomic DNA fragments for complementation experiments. All vectors were derived from pCambia T-DNA cloning vectors, with the exception of a chemically inducible vector, for Agrobacterium sp.-mediated transformation of a wide range of plant species.

  13. Establishing the Empirical Relationship Between Non-Science Majoring Undergraduate Learners' Spatial Thinking Skills and Their Conceptual Astronomy Knowledge. (Spanish Title: Estableciendo Una Relación Empírica Entre el Razonamiento Espacial de los Estudiantes de Graduación de Carreras no Científicas y su Conocimento Conceptual de la Astronomía.) Estabelecendo Uma Relação Empírica Entre o RacioCínio Espacial dos Estudantes de Graduação EM Carreiras Não Científicas e Seu Conhecimento Conceitual da Astronomia

    NASA Astrophysics Data System (ADS)

    Heyer, Inge; Slater, Stephanie J.; Slater, Timothy F.

    2013-12-01

    The astronomy education community has tacitly assumed that learning astronomy is a conceptual domain resting upon spatial thinking skills. As a first step to formally identify an empirical relationship, undergraduate students in a non-major introductory astronomy survey class at a mediumsized, Ph.D. granting, mid-western US university were given pre- and post-astronomy conceptual diagnostics and spatial reasoning diagnostics, Instruments used were the "Test Of Astronomy Standards" and "What Do You Know?" Using only fully matched data for analysis, our sample consisted of 86 undergraduate non-science majors. Students' normalized gains for astronomy surveys were low at .26 and .13 respectively. Students' spatial thinking was measured using an instrument designed specifically for this study. Correlations between the astronomy instruments' pre- to post-course gain scores and the spatial assessment instrument show moderate to strong relationships suggesting the relationship between spatial reasoning and astronomy ability can explain about 25% of the variation in student achievement. La comunidad de educación en astronomía ha supuesto de forma tácita que el aprendizaje de la astronomía consiste en un dominio conceptual fundamentado en el razonamiento espacial. Como un primer paso para identificar formalmente una relación empírica entre estas dos cosas, utilizamos como muestra los estudantes de graduación de carreras no científicas de un curso experimental en una universidad norteamericana del medioeste de porte mediano con programa de Doctorado em curso, en el cual estos estudiantes se sometieron a un diagnóstico de razonamiento espacial y conceptos astronómicos antes e después del mismo. Las herramientas utilizadas fueron el Test Of Astronomy Standards (TOAST) y el cuestionario What do you know? Utilizando solo los datos completamente consistentes para este análisis, nuestra muestra consistió en 86 estudantes de graduación. Las mejoras, depués de

  14. Le rôle du soutien parental dans la relation entre la victimisation homophobe, l’homophobie intériorisée et la détresse psychologique chez les jeunes de minorités sexuelles (JMS) : une approche de médiation modérée

    PubMed Central

    Bergeron, Félix-Antoine; Blais, Martin; Hébert, Martine

    2016-01-01

    Résumé Cet article explore le rôle modérateur du soutien parental dans les relations entre la victimisation homophobe, l’homophobie intériorisée et la détresse psychologique chez des jeunes de minorités sexuelles (JMS), que l’on dit aussi lesbiennes, gais, bisexuels ou en questionnement. Il vise à 1) documenter la prévalence des différentes formes de victimisation homophobe vécue par les JMS, et ce, selon le genre et l’âge et 2) à explorer l’effet modérateur du soutien parental dans la relation entre la victimisation homophobe, l’homophobie intériorisée et la détresse psychologique. Un échantillon de 228 JMS âgés de 14 à 22 ans, non exclusivement hétérosexuels, recrutés en milieu communautaire dans le cadre de l’enquête sur le Parcours Amoureux des Jeunes (PAJ) du Québec a été analysé. L’impact de la victimisation homophobe, du soutien parental, de l’homophobie intériorisée sur la détresse psychologique est exploré par un modèle de régression linéaire avec effets de médiation modérée. Le rôle modérateur du soutien parental est confirmé dans la relation entre la victimisation homophobe et la détresse psychologique. Ces variables peuvent constituer des leviers pour prévenir les effets négatifs des préjudices homophobes sur la santé mentale des JMS. PMID:26966851

  15. [The effect of a warm-up protocol on the sit-and-reach test score in adolescent students].

    PubMed

    Díaz-Soler, María Angeles; Vaquero-Cristóbal, Raquel; Espejo-Antúnez, Luis; López-Miñarro, Pedro Ángel

    2015-06-01

    Introducción: los test lineales son frecuentemente utilizados en las clases de educación física para evaluar la extensibilidad isquiosural del alumnado, sin que haya una estandarización del procedimiento para su realización. Objetivo: analizar la influencia de un protocolo de calentamiento en los resultados obtenidos en el test sit-andreach en estudiantes adolescentes. Metodología: a 47 adolescentes (17 chicos y 30 chicas) se les midió la distancia alcanzada en el test sit-and-reach antes, inmediatamente después de finalizar, así como a los 5 y 10 minutos tras finalizar un protocolo de calentamiento compuesto por una parte de carrera continua, actividades de locomoción y movilidad, además de estiramientos estáticos de cuádriceps, isquiosurales, aductores, psoas ilíaco y gemelos, con una duración total de 8 minutos. Entre las mediciones realizadas tras el calentamiento, los participantes permanecieron en bipedestación sin realizar ejercicio y/o estiramiento alguno. Resultados y discusión: tras el calentamiento hubo una mejora significativa en la distancia alcanzada en el test sit-and-reach (+ 2,15 cm) (p < 0,001), siendo ligeramente mayor a los 5 y 10 minutos (+ 2,49 cm a los 5 minutos y + 2,61 cm a los 10 minutos) (p < 0,001 respecto al pretest). Conclusiones: la realización de un protocolo de calentamiento previo al test sit-and-reach, compuesto por locomoción, movilidad y estiramientos, incrementa de manera significativa la distancia alcanzada en este test, que permanece aumentada, al menos, hasta 10 minutos después de finalizar el calentamiento.

  16. Analysis of PLEs' Implementation under OER Design as a Productive Teaching-Learning Strategy in Higher Education. A Case Study at Universidad Nacional de Educación a Distancia

    ERIC Educational Resources Information Center

    Vázquez-Cano, Esteban; Martín-Monje, Elena; Castrillo de Larreta-Azelain, María Dolores

    2016-01-01

    This paper shows some research which analysed the didactic functionality of Personal Learning Environments (PLEs) and Open Educational Resources (OERs). They were created by students from the Master's Degree in Information and Communication Technologies applied to language teaching and processing, at Universidad Nacional de Educación a Distancia…

  17. DIMED 86: Discurso dos Media e Ensino a Distancia = Discours des Media et Enseignement a Distance = Media Speech and Distance Teaching. Papers Presented at a Seminar (21st, Algarve, Portugal, March 10-15, 1986).

    ERIC Educational Resources Information Center

    Coelho, Maria Eduarda Leal

    Presentations at this seminar on distance education focused on the different types of speech in multimedia presentations which contribute to the elaboration (simulated or substituted) of a situation involving different relationships. In addition to opening and closing remarks by Marcel de Greve and a final report by the Scientific Committee of…

  18. El círculo meridiano automático de San Fernando - San Juan. Sus primeros pasos en el hemisferio sur

    NASA Astrophysics Data System (ADS)

    Mallamaci, C. C.; Muiños, J. L.; Gallego, M.; Pérez, J. A.; Marmolejo, L.; Navarro, J. L.; Sedeño, J.; Vallejos, M.; Belizón, F.

    Se informa sobre el estado actual del Círculo Meridiano Automático de San Fernando-San Juan. El instrumento (Grubb-Parson, de 178mm de abertura y 2665 mm de distancia focal) es gemelo del que se encuentra en las Islas Canarias, y fue instalado durante los meses de julio y agosto de 1996 en la estación astronómica ``Dr. C.U.Cesco" (El Leoncito, Barreal), a unos 200 km de distancia de la ciudad de San Juan, merced a un Convenio de Cooperación Científica, firmado en 1994 entre el ROA (España) y el OAFA (Argentina). En la actualidad se está llevando a cabo un programa de prueba cuyos resultados preliminares muestran que el telescopio está en buenas condiciones para observar estrellas de hasta magnitud aproximada 14.5, con buenos errores de observación (<0.12" en ascensión recta y declinación).

  19. Medición de los parámetros cosmológicos q0, ΩM, y ΩΛ, usando supernovas de Tipo Ia distantes

    NASA Astrophysics Data System (ADS)

    Clocchiatti, A.; High-Z Sne Search Team

    Las supernovas de tipo Ia son una herramienta de gran precisión para la medición de distancias de interés cosmológico. Los métodos recientes de calibración de su luminosidad intrínseca, que hacen uso de la forma de las curvas de luz en varios colores y permiten diferenciar entre supernovas distantes, intrínsecamente débiles, u oscurecidas por extinción, reducen la dispersión del método que las asume de magnitud absoluta constante de 0.50 mag a 0.15 mag, e incrementa el valor de la constante de Hubble de ~55 km s-1 Mpc-1, a 65 km s-1 Mpc-1. A partir de la calibración de las supernovas cercanas, con redshifts menores que 0.1, se pueden obtener distancias precisas a supernovas que explotan a alto redshift. Hemos aplicado estos métodos a 16 supernovas con 0.16 < Z < 0.97, encontrando que sus distancias son, en promedio, entre 10% y 13% (dependiendo del método empleado) mayores que las que uno esperaría en un universo con poca masa (Ω = 0.2), sin una constante cosmológica. Todos los métodos de ajuste de curvas de luz, y selección de subgrupos de la muestra de supernovas observadas, favorecen consistentemente modelos del universo que se expanden eternamente y que tienen una constante cosmológica positiva (ΩΛ > 0), y una aceleración de la expansión al presente (q0 < 0). Hay distintas fuentes de posibles errores sistemáticos que merecen ser analizadas, entre ellas: evolución de la metalicidad y estrellas progenitoras, extinción, bias en la elección de la muestra, amplificación por gravitational lensing, y contaminación de la muestra. Ninguno de estos efectos alcanza para reconciliar los datos con ΩΛ = 0, o q0 > 0.

  20. Nuevas observaciones de cuatro cúmulos abiertos

    NASA Astrophysics Data System (ADS)

    Ahumada, J.; Lapasset, E.

    Presentamos los resultados preliminares de observaciones CCD en cuatro colores (UBVR) de áreas de ~5' de radio centradas en los cúmulos abiertos NGC 2204, NGC 2627, NGC 3247 y NGC 3603. Las observaciones se llevaron a cabo entre los años 1994 y 1996 con el telescopio de 2,15 m del CASLEO y el detector TEK 1024 en su modo directo. Mostramos los diagramas color-magnitud y color-color preliminares de los cúmulos, sobre los que, mediante criterios fotométricos, hacemos una selección de los probables miembros. Asimismo se determinan enrojecimientos, distancias, edades y otros parámetros básicos de los cúmulos.

  1. Sistema Planeta-Satélite. Simulación orbital y potenciales gravitatorios

    NASA Astrophysics Data System (ADS)

    Medina, C.; Carrillo, M.

    Se presenta un programa (desarrollado en Quick Basic 4.5) que simula, en tres dimensiones, el movimiento orbital de un satélite (o luna) alrededor de un planeta, al tiempo que calcula y grafica, en un plano, el potencial gravitatorio del sistema en función de la distancia al planeta. Para la simulación orbital, se emplea la matriz de transformación entre el sistema del planeta y el plano orbital. Para el cálculo y graficación del potencial se aplica un desarrollo en serie hasta el segundo orden, que da cuenta del efecto de achatamiento de los polos, en caso de que éste exista. Las longitudes de los ejes del planeta, la masa de éste y del satélite, sus tamaños aparentes, y los parámetros orbitales son introducidos por el usuario.

  2. HI en la dirección de la denominada ``Ventana de Puppis"

    NASA Astrophysics Data System (ADS)

    Morras, R.; Pöppel, W. G. L.; Arnal, E. M.; Bajaja, E.

    Analizando los datos combinados de los relevamientos de HI de Hartmann & Burton (1997) y Arnal et.al (2000), hemos detectado la presencia de una estructura en forma de ``shell" en la denominada ``ventana de Puppis". Esta estructura, cuyo centro se encuentra en la dirección (l = 245 deg, b = -5 deg) tiene un diámetro angular de ˜ 18 deg y es detectada en el rango de velocidades (LSR) entre -9 y +6 km/seg. El análisis de los datos sugiere que esta estructura puede haberse originado en una perturbación en el denominado ``Feature A" de Lindblad (1973), a una distancia de 150-200 pc del Sol. Se determinaron parámetros tales como Masa y Energía Cinética del Shell y se analiza un posible origen del mismo.

  3. The Arabidopsis cysteine-rich protein GASA4 promotes GA responses and exhibits redox activity in bacteria and in planta.

    PubMed

    Rubinovich, Lior; Weiss, David

    2010-12-01

    Although the gibberellin (GA) signaling pathway has been elucidated, very little is known about the steps linking first transcriptional activation to physiological responses. Among the few identified GA-induced genes are the plant-specific GAST1-like genes, which encode small proteins with a conserved cysteine-rich domain. The role of these proteins in plant development and GA responses is not yet clear. The Arabidopsis GAST1-like gene family consists of 14 members, GASA1-14. Here we show that over-expression of the GA-induced GASA4 gene in Arabidopsis promoted GA responses such as flowering and seed germination. Suppression of several GASA genes using synthetic microRNA (miR(GASA) ) also promoted seed germination. This was probably caused by suppression of GASA5, which acts as a repressor of GA responses. Previously, we proposed that GAST1-like proteins are involved in redox reactions via their cysteine-rich domain. The results of this study support this hypothesis, as over-expression of GASA4 suppressed ROS accumulation and the transgenic seeds were partially resistant to the NO donor sodium nitroprusside (SNP). Moreover, Escherichia coli expressing intact GASA4 or a truncated version containing only the cysteine-rich domain were resistant to SNP. Mutated GASA4, in which conserved cysteines were replaced by alanines, lost its redox activity and the ability to promote GA responses, suggesting that the two functions are linked. We propose that GA induces some GAST1-like genes and suppresses others to regulate its own responses. We also suggest that the encoded proteins regulate the redox status of specific components to promote or suppress these responses.

  4. Closed reference metatranscriptomics enables in planta profiling of putative virulence activities in the grapevine trunk-disease complex

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Grapevines, like other perennial crops, are affected by so-called ‘trunk diseases’, which damage the trunk and other woody tissues. Mature grapevines typically contract more than one trunk disease and often multiple grapevine trunk pathogens (GTPs) are recovered from infected tissues. The co-existen...

  5. Remote sensing of gene expression in Planta: transgenic plants as monitors of exogenous stress perception in extraterrestrial environments

    NASA Technical Reports Server (NTRS)

    Manak, Michael S.; Paul, Anna-Lisa; Sehnke, Paul C.; Ferl, Robert J.

    2002-01-01

    Transgenic arabidopsis plants containing the alcohol dehydrogenase (Adh) gene promoter fused to the green fluorescent protein (GFP) reporter gene were developed as biological sensors for monitoring physiological responses to unique environments. Plants were monitored in vivo during exposure to hypoxia, high salt, cold, and abcissic acid in experiments designed to characterize the utility and responses of the Adh/GFP biosensors. Plants in the presence of environmental stimuli that induced the Adh promoter responded by expressing GFP, which in turn generated a detectable fluorescent signal. The GFP signal degraded when the inducing stimulus was removed. Digital imaging of the Adh/GFP plants exposed to each of the exogenous stresses demonstrated that the stress-induced gene expression could be followed in real time. The experimental results established the feasibility of using a digital monitoring system for collecting gene expression data in real time from Transgenic Arabidopsis Gene Expression System (TAGES) biosensor plants during space exploration experiments.

  6. In planta production of the highly potent resveratrol analogue pterostilbene via stilbene synthase and O-methyltransferase co-expression

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Stilbenoid phytoalexins such as resveratrol, play an important defense role in several plant species against pathogens such as Botrytis cinerea. In addition to their antimicrobial properties, resveratrol and related stilbenes have also generated considerable interest as nutraceuticals, due to their...

  7. Inhibitory effect of Xenorhabdus nematophila TB on plant pathogens Phytophthora capsici and Botrytis cinerea in vitro and in planta.

    PubMed

    Fang, Xiangling; Zhang, Manrang; Tang, Qian; Wang, Yonghong; Zhang, Xing

    2014-03-06

    Entomopathogenic bacteria Xenorhabdus spp. produce secondary metabolites with potential antimicrobial activity for use in agricultural productions. This study evaluated the inhibitory effect of X. nematophila TB culture on plant pathogens Botrytis cinerea and Phytophthora capsici. The cell-free filtrate of TB culture showed strong inhibitory effects (>90%) on mycelial growth of both pathogens. The methanol-extracted bioactive compounds (methanol extract) of TB culture also had strong inhibitory effects on mycelial growth and spore germinations of both pathogens. The methanol extract (1000 μg/mL) and cell-free filtrate both showed strong therapeutic and protective effects (>70%) on grey mold both in detached tomato fruits and plants, and leaf scorch in pepper plants. This study demonstrates X. nematophila TB produces antimicrobial metabolites of strong activity on plant pathogens, with great potential for controlling tomato grey mold and pepper leaf scorch and being used in integrated disease control to reduce chemical application.

  8. An in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum.

    PubMed

    Chooi, Yit-Heng; Krill, Christian; Barrow, Russell A; Chen, Shasha; Trengove, Robert; Oliver, Richard P; Solomon, Peter S

    2015-01-01

    Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-mellein and (R)-O-methylmellein. Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-mellein. The P. nodorum ΔSN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-mellein at 200 μg/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera. Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases.

  9. Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains.

    PubMed

    Rufián, José S; Macho, Alberto P; Corry, David S; Mansfield, John; Ruiz-Albert, Javier; Arnold, Dawn; Beuzón, Carmen R

    2017-01-24

    Recent advances in genomics and single-cell analysis have demonstrated the extraordinary complexity that microbial populations may reach within their hosts. Communities range from complex multispecies groups, to homogeneous populations differentiating into lineages through genetic or non-genetic mechanisms. Diversity within bacterial populations is recognised as a key driver of the evolution of animal pathogens. In plants, however, little is known about how interactions between different pathogenic and non-pathogenic variants within the host impacts on defence responses or how presence within a mixture may affect the development or the fate of each variant. Using confocal fluorescence microscopy, we have analysed the colonization of the plant apoplast by individual virulence variants of Pseudomonas syringae within mixed populations. We found that non-pathogenic variants can proliferate and even spread beyond the inoculated area to neighbouring tissues when in close proximity to pathogenic bacteria. The high bacterial concentrations reached at natural entry points promote such interactions during the infection process. We also found that a diversity of interactions take place at a cellular level between virulent and avirulent variants, ranging from dominant negative effects on proliferation of virulent to in trans suppression of defences triggered by avirulent bacteria. Our results illustrate the spatial dynamics and complexity of the interactions found within mixed infections, and their potential impact on pathogen evolution. This article is protected by copyright. All rights reserved.

  10. A visual identification key utilizing both gestalt and analytic approaches to identification of Carices present in North America (Plantae, Cyperaceae).

    PubMed

    Jones, Timothy Mark

    2013-01-01

    Images are a critical part of the identification process because they enable direct, immediate and relatively unmediated comparisons between a specimen being identified and one or more reference specimens. The Carices Interactive Visual Identification Key (CIVIK) is a novel tool for identification of North American Carex species, the largest vascular plant genus in North America, and two less numerous closely-related genera, Cymophyllus and Kobresia. CIVIK incorporates 1288 high-resolution tiled image sets that allow users to zoom in to view minute structures that are crucial at times for identification in these genera. Morphological data are derived from the earlier Carex Interactive Identification Key (CIIK) which in turn used data from the Flora of North America treatments. In this new iteration, images can be viewed in a grid or histogram format, allowing multiple representations of data. In both formats the images are fully zoomable.

  11. In planta production of the highly potent resveratrol analogue pterostilbene via stilbene synthase and O-methyltransferase co-expression

    SciTech Connect

    Rimando A. M.; Liu C.; Pan, Z.; Polashock, J. J.; Dayan, F. E., Mizuno, C. S.; Snook, M. E.; Baerson, S. R.

    2012-04-01

    Resveratrol and related stilbenes are thought to play important roles in defence responses in several plant species and have also generated considerable interest as nutraceuticals owing to their diverse health-promoting properties. Pterostilbene, a 3,5-dimethylether derivative of resveratrol, possesses properties similar to its parent compound and, additionally, exhibits significantly higher fungicidal activity in vitro and superior pharmacokinetic properties in vivo. Recombinant enzyme studies carried out using a previously characterized O-methyltransferase sequence from Sorghum bicolor (SbOMT3) demonstrated its ability to catalyse the A ring-specific 3,5-bis-O-methylation of resveratrol, yielding pterostilbene. A binary vector was constructed for the constitutive co-expression of SbOMT3 with a stilbene synthase sequence from peanut (AhSTS3) and used for the generation of stably transformed tobacco and Arabidopsis plants, resulting in the accumulation of pterostilbene in both species. A reduced floral pigmentation phenotype observed in multiple tobacco transformants was further investigated by reversed-phase HPLC analysis, revealing substantial decreases in both dihydroquercetin-derived flavonoids and phenylpropanoid-conjugated polyamines in pterostilbene-producing SbOMT3/AhSTS3 events. These results demonstrate the potential utility of this strategy for the generation of pterostilbene-producing crops and also underscore the need for the development of additional approaches for minimizing concomitant reductions in key phenylpropanoid-derived metabolites.

  12. Phytohormonal networks promote differentiation of fiber initials on pre-anthesis cotton ovules grown in vitro and in planta

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The number of cotton (Gossypium sp.) ovule epidermal cells differentiating into fiber initials is an important factor affecting cotton yield and fiber quality. Despite extensive efforts in determining the molecular mechanisms regulating fiber initial differentiation, only a few genes responsible for...

  13. Allelic differences in Medicago truncatula NIP/LATD mutants correlate with their encoded proteins' transport activities in planta.

    PubMed

    Salehin, Mohammad; Huang, Ying-Sheng; Bagchi, Rammyani; Sherrier, D Janine; Dickstein, Rebecca

    2013-02-01

    Medicago truncatula NIP/LATD gene, required for symbiotic nitrogen fixing nodule and root architecture development, encodes a member of the NRT1(PTR) family that demonstrates high-affinity nitrate transport in Xenopus laevis oocytes. Of three Mtnip/latd mutant proteins, one retains high-affinity nitrate transport in oocytes, while the other two are nitrate-transport defective. To further examine the mutant proteins' transport properties, the missense Mtnip/latd alleles were expressed in Arabidopsis thaliana chl1-5, resistant to the herbicide chlorate because of a deletion spanning the nitrate transporter AtNRT1.1(CHL1) gene. Mtnip-3 expression restored chlorate sensitivity in the Atchl1-5 mutant, similar to wild type MtNIP/LATD, while Mtnip-1 expression did not. The high-affinity nitrate transporter AtNRT2.1 gene was expressed in Mtnip-1 mutant roots; it did not complement, which could be caused by several factors. Together, these findings support the hypothesis that MtNIP/LATD may have another biochemical activity.

  14. Purification of Two Novel Sugar Acid-binding Lectins from Haplomitrium Mnioides (bryophyte, Plantae) and their Preliminary Characterization.

    PubMed

    Masuzaki, Hiroaki; Hosono, Masahiro; Nitta, Kazuo

    2017-01-01

    Two novel sugar acid-binding lectins were purified from Haplomitrium mnioides (Lindb.) Schust. using a procedure consisting of ammonium sulfate precipitation, G-50 gel filtration, hydroxyapatite chromatography, and HW-50 gel filtration. We reported their partial physicochemical properties: molecular weight, affinity for carbohydrates and organic acids, pH stability, and dependence of their hemagglutination activity on metal ions. We also determined their N-terminal amino acid sequences. H. mnioides lectins (HMLs) were monomers (one with a molecular weight of approximately 27 kDa, and the other with a molecular weight of approximately 105 kDa) under both nonreducing and reducing conditions. They were named HML27 and HML105, respectively. Both HMLs had an affinity for N-acetylneuraminic acid, D-glucuronic acid, D-glucaric acid, bovine submaxillary mucin, heparin, and organic acids, such as citrate, 2-oxoglutaric acid, and D-2-hydroxyglutarate. Furthermore, HML27 had an affinity for α-D-galacturonic acid, D-malate, L-malate, and pyruvate, while HML105 had an affinity for D-gluconic acid. HML27 and HML105 are novel plant lectins: they have an affinity for sugar acids and organic acids and specifically recognize the carboxyl group, and there is no homology between their N-terminal amino acid sequences and those of the previously described lectins and agglutinins.

  15. An In Planta-Expressed Polyketide Synthase Produces (R)-Mellein in the Wheat Pathogen Parastagonospora nodorum

    PubMed Central

    Krill, Christian; Barrow, Russell A.; Chen, Shasha; Trengove, Robert; Oliver, Richard P.; Solomon, Peter S.

    2014-01-01

    Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-mellein and (R)-O-methylmellein. Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-mellein. The P. nodorum ΔSN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-mellein at 200 μg/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera. Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases. PMID:25326302

  16. Vascular Streak Dieback of cacao in Southeast Asia and Melanesia: in planta detection of the pathogen and a new taxonomy.

    PubMed

    Samuels, Gary J; Ismaiel, Adnan; Rosmana, Ade; Junaid, Muhammad; Guest, David; McMahon, Peter; Keane, Philip; Purwantara, Agus; Lambert, Smilja; Rodriguez-Carres, Marianela; Cubeta, Marc A

    2012-01-01

    Vascular Streak Dieback (VSD) disease of cacao (Theobroma cacao) in Southeast Asia and Melanesia is caused by a basidiomycete (Ceratobasidiales) fungus Oncobasidium theobromae (syn. =Thanatephorus theobromae). The most characteristic symptoms of the disease are green-spotted leaf chlorosis or, commonly since about 2004, necrotic blotches, followed by senescence of leaves beginning on the second or third flush behind the shoot apex, and blackening of infected xylem in the vascular traces at the leaf scars resulting from the abscission of infected leaves. Eventually the shoot apex is killed and infected branches die. In susceptible cacao the fungus may grow through the xylem down into the main stem and kill a mature cacao tree. Infections in the stem of young plants prior to the formation of the first 3-4 lateral branches usually kill the plant. Basidiospores released from corticioid basidiomata developed on leaf scars or along cracks in the main vein of infected leaves infect young leaves. The pathogen commonly infects cacao but there are rare reports from avocado. As both crops are introduced to the region, the pathogen is suspected to occur asymptomatically in native vegetation. The pathogen is readily isolated but cultures cannot be maintained. In this study, DNA was extracted from pure cultures of O. theobromae obtained from infected cacao plants sampled from Indonesia. The internal transcribed spacer region (ITS), consisting of ITS1, 5.8S ribosomal RNA and ITS2, and a portion of nuclear large subunit (LSU) were sequenced. Phylogenetic analysis of ITS sequences placed O. theobromae sister to Ceratobasidium anastomosis groups AG-A, AG-Bo, and AG-K with high posterior probability. Therefore the new combination Ceratobasidium theobromae is proposed. A PCR-based protocol was developed to detect and identify C. theobromae in plant tissue of cacao enabling early detection of the pathogen in plants. A second species of Ceratobasidium, Ceratobasidium ramicola, identified through ITS sequence analysis, was isolated from VSD-affected cacao plants in Java, and is widespread in diseased cacao collected from Indonesia.

  17. Production and characterization of in planta transiently produced polygalacturanase from Aspergillus niger and its fusions with hydrophobin or ELP tags

    PubMed Central

    2014-01-01

    Background Pectinases play an important role in plant cell wall deconstruction and have potential in diverse industries such as food, wine, animal feed, textile, paper, fuel, and others. The demand for such enzymes is increasing exponentially, as are the efforts to improve their production and to implement their use in several industrial processes. The goal of this study was to examine the potential of producing polygalacturonase I from Aspergillus niger in plants and to investigate the effects of subcellular compartmentalization and protein fusions on its accumulation and activity. Results Polygalacturonase I from Aspergillus niger (AnPGI) was transiently produced in Nicotiana benthamiana by targeting it to five different cellular compartments: apoplast, endoplasmic reticulum (ER), vacuole, chloroplast and cytosol. Accumulation levels of 2.5%, 3.0%, and 1.9% of total soluble protein (TSP) were observed in the apoplast, ER, and vacuole, respectively, and specific activity was significantly higher in vacuole-targeted AnPGI compared to the same enzyme targeted to the ER or apoplast. No accumulation was found for AnPGI when targeted to the chloroplast or cytosol. Analysis of AnPGI fused with elastin-like polypeptide (ELP) revealed a significant increase in the protein accumulation level, especially when targeted to the vacuole where the protein doubles its accumulation to 3.6% of TSP, while the hydrophobin (HFBI) fusion impaired AnPGI accumulation and both tags impaired activity, albeit to different extents. The recombinant protein showed activity against polygalacturonic acid with optimum conditions at pH 5.0 and temperature from 30 to 50°C, depending on its fusion. In vivo analysis of reducing sugar content revealed a higher release of reducing sugars in plant tissue expressing recombinant AnPGI compared to wild type N. benthamiana leaves. Conclusion Our results demonstrate that subcellular compartmentalization of enzymes has an impact on both the target protein accumulation and its activity, especially in the case of proteins that undergo post-translational modifications, and should be taken into consideration when protein production strategies are designed. Using plants to produce heterologous enzymes for the degradation of a key component of the plant cell wall could reduce the cost of biomass pretreatment for the production of cellulosic biofuels. PMID:24970673

  18. Inhibitory effect of Xenorhabdus nematophila TB on plant pathogens Phytophthora capsici and Botrytis cinerea in vitro and in planta

    PubMed Central

    Fang, Xiangling; Zhang, Manrang; Tang, Qian; Wang, Yonghong; Zhang, Xing

    2014-01-01

    Entomopathogenic bacteria Xenorhabdus spp. produce secondary metabolites with potential antimicrobial activity for use in agricultural productions. This study evaluated the inhibitory effect of X. nematophila TB culture on plant pathogens Botrytis cinerea and Phytophthora capsici. The cell-free filtrate of TB culture showed strong inhibitory effects (>90%) on mycelial growth of both pathogens. The methanol-extracted bioactive compounds (methanol extract) of TB culture also had strong inhibitory effects on mycelial growth and spore germinations of both pathogens. The methanol extract (1000 μg/mL) and cell-free filtrate both showed strong therapeutic and protective effects (>70%) on grey mold both in detached tomato fruits and plants, and leaf scorch in pepper plants. This study demonstrates X. nematophila TB produces antimicrobial metabolites of strong activity on plant pathogens, with great potential for controlling tomato grey mold and pepper leaf scorch and being used in integrated disease control to reduce chemical application. PMID:24599183

  19. Indole-3-acetic acid in plant-pathogen interactions: a key molecule for in planta bacterial virulence and fitness.

    PubMed

    Cerboneschi, Matteo; Decorosi, Francesca; Biancalani, Carola; Ortenzi, Maria Vittoria; Macconi, Sofia; Giovannetti, Luciana; Viti, Carlo; Campanella, Beatrice; Onor, Massimo; Bramanti, Emilia; Tegli, Stefania

    The plant pathogenic bacterium Pseudomonas savastanoi, the causal agent of olive and oleander knot disease, uses the so-called "indole-3-acetamide pathway" to convert tryptophan to indole-3-acetic acid (IAA) via a two-step pathway catalyzed by enzymes encoded by the genes in the iaaM/iaaH operon. Moreover, pathovar nerii of P. savastanoi is able to conjugate IAA to lysine to generate the less biologically active compound IAA-Lys via the enzyme IAA-lysine synthase encoded by the iaaL gene. Interestingly, iaaL is now known to be widespread in many Pseudomonas syringae pathovars, even in the absence of the iaaM and iaaH genes for IAA biosynthesis. Here, two knockout mutants, ΔiaaL and ΔiaaM, of strain Psn23 of P. savastanoi pv. nerii were produced. Pathogenicity tests using the host plant Nerium oleander showed that ΔiaaL and ΔiaaM were hypervirulent and hypovirulent, respectively and these features appeared to be related to their differential production of free IAA. Using the Phenotype Microarray approach, the chemical sensitivity of these mutants was shown to be comparable to that of wild-type Psn23. The main exception was 8 hydroxyquinoline, a toxic compound that is naturally present in plant exudates and is used as a biocide, which severely impaired the growth of ΔiaaL and ΔiaaM, as well as growth of the non-pathogenic mutant ΔhrpA, which lacks a functional Type Three Secretion System (TTSS). According to bioinformatics analysis of the Psn23 genome, a gene encoding a putative Multidrug and Toxic compound Extrusion (MATE) transporter, was found upstream of iaaL. Similarly to iaaL and iaaM, its expression appeared to be TTSS-dependent. Moreover, auxin-responsive elements were identified for the first time in the modular promoters of both the iaaL gene and the iaaM/iaaH operon of P. savastanoi, suggesting their IAA-inducible transcription. Gene expression analysis of several genes related to TTSS, IAA metabolism and drug resistance confirmed the presence of a concerted regulatory network in this phytopathogen among virulence, fitness and drug efflux.

  20. In planta mechanism of action of leptospermone: impact of its physico-chemical properties on uptake, translocation, and metabolism

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Leptospermone is a natural ß-triketone that specifically inhibits the enzyme p-hydrophyphenylpyruvate dioxygenase, the same molecular target site as that of the commercial herbicide mesotrione. The ß-triketone-rich essential oil of Leptospermum scoparium has both preemergence or postemergence herbi...

  1. BacS: an abundant bacteroid protein in Rhizobium etli whose expression ex planta requires nifA.

    PubMed

    Jahn, Olivia J; Davila, Guillermo; Romero, David; Noel, K Dale

    2003-01-01

    Rhizobium etli CFN42 bacteroids from bean nodules possessed an abundant 16-kDa protein (BacS) that was found in the membrane pellet after cell disruption. This protein was not detected in bacteria cultured in tryptone-yeast extract. In minimal media, it was produced at low oxygen concentration but not in a mutant whose nifA was disrupted. N-terminal sequencing of the protein led to isolation of a bacS DNA fragment. DNA hybridization and nucleotide sequencing revealed three copies of the bacS gene, all residing on the main symbiotic plasmid of strain CFN42. A stretch of 304 nucleotides, exactly conserved upstream of all three bacS open reading frames, had very close matches with the NifA and sigma 54 consensus binding sequences. The only bacS homology in the genetic sequence databases was to three hypothetical proteins of unknown function, all from rhizobial species. Mutation and genetic complementation indicated that each of the bacS genes gives rise to a BacS polypeptide. Mutants disrupted or deleted in all three genes did not produce the BacS polypeptide but were Nod+ and Fix+ on Phaseolus vulgaris.

  2. Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance In Planta

    PubMed Central

    Lafi, Feras F.; Ramirez-Prado, Juan S.; Alam, Intikhab; Bajic, Vladimir B.

    2016-01-01

    Halomonas elongata strain K4 is an endophytic bacterial strain that was isolated from roots of Cyperus conglomeratus collected at the Red Sea coast in Thuwal, Saudi Arabia. Here, we present a draft genome sequence of this strain, highlighting a number of pathways involved in plant growth promotion under salt stress. PMID:27811099

  3. Health risk assessment of air emissions from a municipal solid waste incineration plant--a case study.

    PubMed

    Cangialosi, Federico; Intini, Gianluca; Liberti, Lorenzo; Notarnicola, Michele; Stellacci, Paolo

    2008-01-01

    A health risk assessment of long-term emissions of carcinogenic and non-carcinogenic air pollutants has been carried out for the municipal solid waste incinerator (MSWI) of the city of Taranto, Italy. Ground level air concentrations and soil deposition of carcinogenic (Polychlorinated Dibenzo-p-Dioxins/Furans and Cd) and non-carcinogenic (Pb and Hg) pollutants have been estimated using a well documented atmospheric dispersion model. Health risk values for air inhalation, dermal contact, soil and food ingestion have been calculated based on a combination of these concentrations and a matrix of environmental exposure factors. Exposure of the surrounding population has been addressed for different release scenarios based on four pollutants, four exposure pathways and two receptor groups (children and adults). Spatial risk distribution and cancer excess cases projected from plant emissions have been compared with background mortality records. Estimated results based on the MSWI emissions show: (1) individual risks well below maximum acceptable levels, (2) very small incremental cancer risk compared with background level.

  4. PLAN-TA9-2443(U), Rev. B Remediated Nitrate Salt (RNS) Surrogate Formulation and Testing Standard Procedure

    SciTech Connect

    Brown, Geoffrey Wayne

    2016-03-16

    This document identifies scope and some general procedural steps for performing Remediated Nitrate Salt (RNS) Surrogate Formulation and Testing. This Test Plan describes the requirements, responsibilities, and process for preparing and testing a range of chemical surrogates intended to mimic the energetic response of waste created during processing of legacy nitrate salts. The surrogates developed are expected to bound1 the thermal and mechanical sensitivity of such waste, allowing for the development of process parameters required to minimize the risk to worker and public when processing this waste. Such parameters will be based on the worst-case kinetic parameters as derived from APTAC measurements as well as the development of controls to mitigate sensitivities that may exist due to friction, impact, and spark. This Test Plan will define the scope and technical approach for activities that implement Quality Assurance requirements relevant to formulation and testing.

  5. "Lo Ultimo": "Consejos"--"Un Dialogo Respetoso Entre Colegas"

    ERIC Educational Resources Information Center

    Vasquez, Olga A.; Flores, Belinda Bustos; Clark, Ellen Riojas

    2001-01-01

    "Los testimonios," or life stories, shared in this volume reveal that academia represents a labyrinth of challenges for aspiring and emerging Latina scholars--a story these authors know all too well. As Latina "veterana" scholars, who have traveled this arduous journey, the authors recognize that their collective efforts have…

  6. Plano Entrópico de galaxias tempranas

    NASA Astrophysics Data System (ADS)

    Volpe, M. G.; Alonso, M. V.; García Lambas, D.

    Early-type galaxies are systems in quasi-equilibrium and we can assume that their specific entropy is constant. This property is reflected in the ob- served correlations among photometric parameters describing the galaxy profiles. We discuss preliminary results of computing the specific entropy in several clusters and groups of galaxies. The galaxy sample is a subsam- ple of the spectro-photometric ENEAR survey (da Costa et al. 2000) of early-type galaxies in the local Universe. The Entropic Plane would allow us to understand the Fundamental Plane of early-type galaxies in dynamical terms and the processes involved in such relationships. FULL TEXT IN SPANISH

  7. Optics Microwave Interactions (Interactions entre optique et micro-ondes)

    DTIC Science & Technology

    2003-04-01

    Dexter JL., Goldberg L., Parent MG., Stilwell D and Cooper DG, IEEE Photon.Tech.Lett.,5, pp 1347 – 1349 (1993). 36 - Dolfi D., Michel- Gabriel F., Bonn S...Chance, M. Cope, E. Gratton , N. Ramanujam, B. Tromberg, “Phase measurement of light absorption and scatter in human tissue,” Rev. of Sci. Inst. 69...Chance, “MRI-guided diffuse optical spectroscopy of malignant and benign breast lesions,” Neoplasia (in press). [30] J.B. Fishkin, and E. Gratton

  8. "Entre Familia": Immigrant Parents' Strategies for Involvement in Children's Schooling

    ERIC Educational Resources Information Center

    Poza, Luis; Brooks, Maneka Deanna; Valdés, Guadalupe

    2014-01-01

    Teachers and administrators in schools with large, working-class Latino populations often complain of parents' indifference or lack of involvement in children's schooling because of their low visibility at school events and relatively little face-to-face communication with teachers and school administration. In a series of semi-structured…

  9. A Astronomia no Ensino Superior Brasileiro entre 1808 e 1889

    NASA Astrophysics Data System (ADS)

    Bretones, Paulo S.; Videira, Antonio A. P.

    2003-05-01

    Este artigo apresenta os principais eventos ocorridos na história do ensino de astronomia nos cursos superiores que existiram no Brasil desde a chegada da Família Real portuguesa em 1808 até o final do período monárquico. Para compor esse esboço histórico, utilizamos, principalmente, livros didáticos, regulamentos, decretos e leis responsáveis pelas organizações dos conteúdos oferecidos e das carreiras dos responsáveis pela disciplina. Na análise do material empregado, investigamos a presença de concepções filosóficas e científicas, que podem ter norteado os conteúdos disciplinares. Não realizamos nenhuma comparação com o ensino de astronomia em outros países. Concluímos mostrando que o estudo de astronomia, durante o período monárquico, foi mais direcionado para a formação d engenheiros do que astrônomos. Gostaríamos de observar que o presente artigo não tem a pretensão de abordar o assunto de maneira completa e detalhada.

  10. Evaluacion entre colegas profesores (Peer Review of Teachers). ERIC Digest.

    ERIC Educational Resources Information Center

    Hertling, Elizabeth

    This digest in Spanish examines peer review, focusing on how these types of reviews can improve teacher competence. Peer review is often linked to peer assistance, which helps new and veteran teachers improve their knowledge and skills. In peer-review programs, consulting teachers conduct formal evaluations and recommend whether the participating…

  11. Etude de la flore bactérienne contaminant les téléphones mobiles avant et après la désinfection: comparaison entre les professionnels soignants de l'hôpital militaire d'instruction Mohammed V de Rabat et les témoins

    PubMed Central

    Uwingabiye, Jean; Moustanfii, Wafaa; Chadli, Meryem; Sekhsokh, Yassine

    2015-01-01

    Introduction L'objectif de notre travail était évaluer la contamination microbienne des téléphones mobiles utilisés par les personnels soignants des différents services de l'hôpital militaire d'instructions Mohammed V de Rabat et la comparer à celui d'une population témoin et aussi démontrer l'efficacité des solutions hydroalcoolique dans la désinfection de ces téléphones mobiles. Méthodes Il s'agit d'une étude descriptive transversale réalisée sur une période de 9 mois entre septembre 2010 et juin 2011, dans le service de bactériologie de l'hôpital militaire d'Instruction Mohammed V. Résultats L’étude bactériologique a été faite sur 240 téléphones mobiles dont 50% provenaient de personnels de sante. Le taux de contamination bactérienne de tous les téléphones mobiles était de 100%. Les cultures des bactéries isolées au niveau des téléphones mobiles du personnel médical étaient plus polymorphes que celles de la population témoin (p=0,028). Parmi 437 bactéries isolées: 223(51%) provenaient de téléphones de personnels de santé et 214(49%) de téléphones de la population témoin avec une différence qui n’était pas statistiquement significative(p>0,05) sauf pour les isolats de Staphylocoque à coagulase négative et Staphylococcus aureus. Les bactéries isolées étaient représentées par: Staphylocoque à coagulase (57,7%), Staphylococcus aureus (18,1%), Corynebacterium sp (18,8%), Bacillus sp (2,3%) et autres (2,2%). La différence entre la prévalence des bactéries isolées selon les services et les fonctions des personnels de santé n’était pas statistiquement significative (p>0,05). La désinfection des téléphones portables par la solution hydroalcoolique a réduit à 99,5% le nombre des colonies. Conclusion Ce travail montre que les téléphones portables pourraient jouer un rôle dans la transmission des infections nosocomiales et communautaires. Dans le cadre de prévention de ces risques, il faut

  12. Toxicité sérotoninergique résultant d’une interaction médicamenteuse entre le bleu de méthylène et les inhibiteurs de la recapture de la sérotonine

    PubMed Central

    Charbonneau, Annie

    2013-01-01

    RÉSUMÉ Contexte : Le bleu de méthylène est employé en pratique pour diverses raisons médicales. Des données récentes ont évoqué une interaction potentielle avec les inhibiteurs de la recapture de la sérotonine, pouvant conduire à une toxicité sérotoninergique. Objectif : Décrire le risque de toxicité sérotoninergique associé à l’interaction entre le bleu de méthylène et les inhibiteurs de la recapture de la sérotonine. Sources de l’information : Les publications pertinentes ont été ciblées systématiquement au moyen des moteurs de recherche MEDLINE (1946 au 21 mars 2013) et Embase (1974 à 2013, semaine 11) en utilisant les mots clés suivants : methylene blue, methylthioninium, monoamine oxidase inhibitors, serotonin reuptake inhibitors et serotonin syndrome. Aucune restriction touchant l’indication du bleu de méthylène ou la langue n’a été appliquée. Les références des publications ont également été analysées. Sélection des études et extraction des données : Dix-huit études de cas et deux séries de cas systématiques ont été sélectionnées. Aucune étude clinique aléatoire n’a encore été publiée. Synthèse des résultats : La première étude de cas à avoir soupçonné une interaction entre le bleu de méthylène et les inhibiteurs de la recapture de la sérotonine est parue en 2003. Dix-sept autres études de cas décrivant le même type d’interaction ont par la suite été rapportées. Les deux séries de cas ont regroupé les données de quelques 325 parathyroïdectomies où le bleu de méthylène avait été employé comme agent colorant. Les 17 patients qui ont présenté une toxicité du système nerveux central prenaient tous en période préopératoire des inhibiteurs de recapture de la sérotonine. Conclusion : Lorsqu’il est administré en concomitance avec des inhibiteurs de recapture de la sérotonine, le bleu de méthylène peut conduire à une toxicité sérotoninergique à une dose aussi

  13. [Family factors influence active commuting to school in Spanish children].

    PubMed

    Rodríguez-López, Carlos; Villa-González, Emilio; Pérez-López, Isaac J; Delgado-Fernández, Manuel; Ruiz, Jonatan R; Chillón, Palma

    2013-01-01

    Introducción: El desplazamiento activo al colegio contribuye a aumentar los niveles de actividad física en niños. Los factores familiares pueden determinar dicho comportamiento. Objetivo: El objetivo fue analizar la asociación de la actividad laboral y el desplazamiento al trabajo de los padres con el modo de desplazamiento de sus hijos. Método: Participaron 721 familias de 4 colegios de la provincia de Granada. Las familias completaron un cuestionario sobre el modo de desplazamiento de sus hijos, la actividad laboral y el modo de desplazamiento de los padres, y la distancia y tiempo del trayecto al colegio de sus hijos. Las asociaciones entre la actividad laboral de las familias y modo de desplazamiento al trabajo con el desplazamiento activo al colegio de sus hijos se estudiaron con regresión logística binaria ajustando por distancia al colegio y edad de los hijos. Resultados: Los niños cuyos padres y madres no trabajaban eran más propensos a ir de forma activa al colegio que aquellos donde ambos trabajaban (p = 0,023; OR: 2,67; 95% IC: 1,14-6,23). Los niños cuyos padres y madres se desplazaban de forma activa al trabajo eran más propensos a ir de forma activa al colegio que aquellos donde ambos padres se desplazaban de forma pasiva al trabajo (p = 0,014; OR: 6,30; 95% IC: 1,45-27,26). Conclusión: Los factores familiares estaban relacionados con el modo de desplazamiento de los niños al colegio: en familias con desempleo y en familias con empleo donde los padres se desplazan al trabajo de forma activa, los hijos parecen ser más activos.

  14. [Not Available].

    PubMed

    Villa-González, Emilio; Rodríguez-López, Carlos; Barranco-Ruiz, Yaira; Cabezas-Arévalo, Luis Fabián; Chillón, Palma

    2016-06-30

    Objetivo: analizar la concordancia de dos métodos de medición (Google MapsTM vs. Sistema de Información Geográfica) para la determinación de la distancia desde el domicilio familiar hasta el colegio.Métodos: un total de 542 escolares de entre 8-11 años de edad (media = 9,36 ± 0,6) del sur de España participaron en el estudio, facilitando la dirección de su domicilio familiar. La distancia desde el domicilio familiar al colegio se calculó mediante la utilización de dos programas diferentes:Google MapsTM y Sistema de Información Geográfica (GIS) en ruta y en línea recta. La asociación entre ambos métodos fue analizada utilizando la correlación de Spearman y el grado de acuerdo a través del coeficiente de correlación intraclase (ICC), así como el método Bland Altman.Resultados: la correlación entre ambos métodos de medición propuestos fue muy significativa (r = 0,966, p < 0,001; r = 0,984, p < 0,001; y r = 0,954, p < 0,001, respectivamente), y la concordancia fue excelente (ICC = 0,96, p < 0,001; ICC = 0,92, p < 0,001; ICC = 0,97, p < 0,001).Conclusiones: los métodos de medición estudiados podrían ser utilizados en función de las necesidades de la investigación, al presentar ambos una alta concordancia. Sin embargo, se recomienda la utilización del Sistema de Información Geográfica en ruta si se cuenta con medios yfinanciación, por tratarse de un método constatado en fiabilidad y validez.

  15. Teaching Reading Comprehension in English in a Distance Web-Based Course: New Roles for Teachers (La enseñanza de la comprensión de lectura en inglés en un curso a distancia en la red: nuevos roles para los docentes)

    ERIC Educational Resources Information Center

    Muñoz Marín, Jorge Hugo; González Moncada, Adriana

    2010-01-01

    Distance web-based learning is a popular strategy in ELT teaching in Colombia. Despite of the growth of experiences, there are very few studies regarding teachers' participation in these courses. This paper reports preliminary findings of an on-going study aiming at exploring the roles that a teach er plays in an EFL reading comprehension distance…

  16. Motivation Conditions in a Foreign Language Reading Comprehension Course Offering Both a Web-Based Modality and a Face-to-Face Modality (Las condiciones de motivación en un curso de comprensión de lectura en lengua extranjera (LE) ofrecido tanto en la modalidad presencial como en la modalidad a distancia en la web)

    ERIC Educational Resources Information Center

    Lopera Medina, Sergio

    2014-01-01

    Motivation plays an important in role in education. Based on the ten macro-strategies proposed by Dörnyei and Csizér (1998), this article analyzes the motivation conditions in a foreign language reading comprehension course using both a web-based modality and a face-to-face modality. A case study was implemented as the primary research method, and…

  17. [Residual effect of muscle strength exercise in secondary prevention of children obesity].

    PubMed

    Vásquez, Fabián; Díaz, Erik; Lera, Lydia; Meza, Jorge; Salas, Isabel; Rojas, Pamela; Atalah, Eduardo; Burrows, Raquel

    2013-01-01

    Introducción: La elevada prevalencia de la obesidad en escolares chilenos (23,1%), hace necesario la aplicación de intervenciones que incorporen el ejercicio físico de fuerza muscular, pues éste muestra gran eficacia en niños obesos. Objetivo: Evaluar el efecto residual del ejercicio físico de fuerza muscular sobre la grasa corporal, el síndrome metabólico y la condición física en escolares obesos. Métodos: La muestra incluyó 111 escolares obesos, entre 8 y 13 AÑOs, de 3 colegios de la ciudad de Santiago. La intervención temprana (n = 60) ejecutó en paralelo ejercicio físico de fuerza muscular, educación alimentaria y apoyo psicológico durante 3 meses. La intervención tardía (n = 51), incorporó los primeros 3 meses, sólo la intervención educativa y el apoyo psicológico, y el ejercicio se agregó entre los 3 y 6 meses. A los 9 meses post-intervención, se evaluó el efecto residual del ejercicio físico. La grasa corporal se determinó por ecuaciones antropométricas. El síndrome metabólico se diagnóstico por el criterio de Cook. La condición física se evaluó por la distancia recorrida en el test de seis minutos. Resultados: El porcentaje grasa corporal disminuyó al final de la intervención y aumentó post-intervención, con la excepción de los hombres de la intervención temprana. La distancia recorrida se incrementó al final de la intervención, pero se redujo en la post-intervención. El síndrome metabólico, disminuyó al término de la intervención incrementando post-intervención (p < 0,05). Conclusiones: La sustentabilidad del ejercicio es fundamental para mantener los cambios, lo que se comprueba al evaluar el efecto residual del ejercicio físico sobre la grasa corporal, el síndrome metabólico y la condición física.

  18. Courtship behavior of different wild strains of Ceratitis Capitata (Diptera: Tephritidae)

    SciTech Connect

    Briceno, D.; Eberhard, W.; Vilardi, J.; Cayol, J.-P.; Shelly, T.

    2007-03-15

    This study documents differences in the courtship behavior of wild strains of Ceratitis capitata (Wiedemann) from Madeira (Portugal), Hawaii (U.S.A.), Costa Rica, and Patagonia (Argentina). Some traits showed large variations and others substantial overlaps. The angle at which the male faced toward the female at the moment of transition from continuous wing vibration and intermittent buzzing changed very little during the course of courtship in all strains, but males from Madeira tended to face more directly toward the female than other males. Females tended to look more, and more directly, toward the males as courtship progressed in all strains. The distance between male and female tended to decrease as courtship proceeded in all strains, but the distances at which males initiated continuous vibration, intermittent buzzing, and jumped onto the female were relatively less variable between strains, except for the strain from Costa Rica. Flies of Madeira courted for longer and the male moved his head and buzzed his wings longer than the other strains. (author) [Spanish] Este estudio documenta diferencias en el comportamiento de cortejo de cepas silvestres de Ceratitis capitata (Wied.) provenientes de Madeira (Portugal), Hawaii (Estados Unidos de Norte America), Costa Rica y Patagonia (Argentina). Algunas caracteristicas mostraron grandes variaciones y traslape substancial. Los angulos a los cuales los machos miraron hacia las hembras cambiaron muy poco en el momento de la transicion de la vibracion continua al zumbido intermitente durante el curso del cortejo en todo las cepas, pero los machos de Madeira tendieron a enfrentar mas directamente a la hembra que otros machos. Los angulos de las hembras disminuyeron claramente durante el cortejo en todas las cepas. La distancia entre el macho y la hembra tendio a disminuir conforme el cortejo continuaba en todas las cepas, pero las distancias a las cuales los machos iniciaron la vibracion continua, el zumbido intermitente

  19. Vascular Streak Dieback of cacao in Southeast Asia detection and Melanesia: in planta detection of the pathogen and a new taxonomy

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vascular Streak Dieback (VSD) disease of cacao (Theobroma cacao) in Southeast Asia and Melanesia is caused by a basidiomycete (Ceratobasidiales) fungus described in a monotypic genus as Oncobasidium theobromae (syn. =Thanatephorus theobromae). The symptoms of the disease include green-spotted chloro...

  20. Regulon studies and in planta role of the BraI/R quorum-sensing system in the plant-beneficial Burkholderia cluster.

    PubMed

    Coutinho, Bruna G; Mitter, Birgit; Talbi, Chouhra; Sessitsch, Angela; Bedmar, Eulogio J; Halliday, Nigel; James, Euan K; Cámara, Miguel; Venturi, Vittorio

    2013-07-01

    The genus Burkholderia is composed of functionally diverse species, and it can be divided into several clusters. One of these, designated the plant-beneficial-environmental (PBE) Burkholderia cluster, is formed by nonpathogenic species, which in most cases have been found to be associated with plants. It was previously established that members of the PBE group share an N-acyl-homoserine lactone (AHL) quorum-sensing (QS) system, designated BraI/R, that produces and responds to 3-oxo-C14-HSL (OC14-HSL). Moreover, some of them also possess a second AHL QS system, designated XenI2/R2, producing and responding to 3-hydroxy-C8-HSL (OHC8-HSL). In the present study, we performed liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis to determine which AHL molecules are produced by each QS system of this group of bacteria. The results showed that XenI2/R2 is mainly responsible for the production of OHC8-HSL and that the BraI/R system is involved in the production of several different AHLs. This analysis also revealed that Burkholderia phymatum STM815 produces greater amounts of AHLs than the other species tested. Further studies showed that the BraR protein of B. phymatum is more promiscuous than other BraR proteins, responding equally well to several different AHL molecules, even at low concentrations. Transcriptome studies with Burkholderia xenovorans LB400 and B. phymatum STM815 revealed that the BraI/R regulon is species specific, with exopolysaccharide production being the only common phenotype regulated by this system in the PBE cluster. In addition, BraI/R was shown not to be important for plant nodulation by B. phymatum strains or for endophytic colonization and growth promotion of maize by B. phytofirmans PsJN.

  1. Regulon Studies and In Planta Role of the BraI/R Quorum-Sensing System in the Plant-Beneficial Burkholderia Cluster

    PubMed Central

    Coutinho, Bruna G.; Mitter, Birgit; Talbi, Chouhra; Sessitsch, Angela; Bedmar, Eulogio J.; Halliday, Nigel; James, Euan K.; Cámara, Miguel

    2013-01-01

    The genus Burkholderia is composed of functionally diverse species, and it can be divided into several clusters. One of these, designated the plant-beneficial-environmental (PBE) Burkholderia cluster, is formed by nonpathogenic species, which in most cases have been found to be associated with plants. It was previously established that members of the PBE group share an N-acyl-homoserine lactone (AHL) quorum-sensing (QS) system, designated BraI/R, that produces and responds to 3-oxo-C14-HSL (OC14-HSL). Moreover, some of them also possess a second AHL QS system, designated XenI2/R2, producing and responding to 3-hydroxy-C8-HSL (OHC8-HSL). In the present study, we performed liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis to determine which AHL molecules are produced by each QS system of this group of bacteria. The results showed that XenI2/R2 is mainly responsible for the production of OHC8-HSL and that the BraI/R system is involved in the production of several different AHLs. This analysis also revealed that Burkholderia phymatum STM815 produces greater amounts of AHLs than the other species tested. Further studies showed that the BraR protein of B. phymatum is more promiscuous than other BraR proteins, responding equally well to several different AHL molecules, even at low concentrations. Transcriptome studies with Burkholderia xenovorans LB400 and B. phymatum STM815 revealed that the BraI/R regulon is species specific, with exopolysaccharide production being the only common phenotype regulated by this system in the PBE cluster. In addition, BraI/R was shown not to be important for plant nodulation by B. phymatum strains or for endophytic colonization and growth promotion of maize by B. phytofirmans PsJN. PMID:23686262

  2. In Planta Recognition of a Double-Stranded RNA Synthesis Protein Complex by a Potexviral RNA Silencing Suppressor[C][W][OPEN

    PubMed Central

    Okano, Yukari; Senshu, Hiroko; Hashimoto, Masayoshi; Neriya, Yutaro; Netsu, Osamu; Minato, Nami; Yoshida, Tetsuya; Maejima, Kensaku; Oshima, Kenro; Komatsu, Ken; Yamaji, Yasuyuki; Namba, Shigetou

    2014-01-01

    RNA silencing plays an important antiviral role in plants and invertebrates. To counteract antiviral RNA silencing, most plant viruses have evolved viral suppressors of RNA silencing (VSRs). TRIPLE GENE BLOCK PROTEIN1 (TGBp1) of potexviruses is a well-characterized VSR, but the detailed mechanism by which it suppresses RNA silencing remains unclear. We demonstrate that transgenic expression of TGBp1 of plantago asiatica mosaic virus (PlAMV) induced developmental abnormalities in Arabidopsis thaliana similar to those observed in mutants of SUPPRESSOR OF GENE SILENCING3 (SGS3) and RNA-DEPENDENT RNA POLYMERASE6 (RDR6) required for the trans-acting small interfering RNA synthesis pathway. PlAMV-TGBp1 inhibits SGS3/RDR6-dependent double-stranded RNA synthesis in the trans-acting small interfering RNA pathway. TGBp1 interacts with SGS3 and RDR6 and coaggregates with SGS3/RDR6 bodies, which are normally dispersed in the cytoplasm. In addition, TGBp1 forms homooligomers, whose formation coincides with TGBp1 aggregation with SGS3/RDR6 bodies. These results reveal the detailed molecular function of TGBp1 as a VSR and shed new light on the SGS3/RDR6-dependent double-stranded RNA synthesis pathway as another general target of VSRs. PMID:24879427

  3. Taxonomic Treatment of Solanum Section Petota (Wild Potatoes) in Catálogo de Plantas Vasculares del Cono Sur (Argentina, Chile, Paraguay, Uruguay, y sur del Brasil)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Solanum section Petota (Solanaceae), which includes the cultivated potato (Solanum tuberosum) and its wild relatives, contains over 150 wild species distributed from the southwestern U.S.A. (38°N) to central Argentina and adjacent Chile (41°S). This catalog includes all species from the Southern Con...

  4. Seed development and maturation in early spring-flowering Galanthus nivalis and Narcissus pseudonarcissus continues post-shedding with little evidence of maturation in planta

    PubMed Central

    Newton, Rosemary J.; Hay, Fiona R.; Ellis, Richard H.

    2013-01-01

    Background and Aims Seeds of the moist temperate woodland species Galanthus nivalis and Narcissus pseudonarcissus, dispersed during spring or early summer, germinated poorly in laboratory tests. Seed development and maturation were studied to better understand the progression from developmental to germinable mode in order to improve seed collection and germination practices in these and similar species. Methods Phenology, seed mass, moisture content and ability to germinate and tolerate desiccation were monitored during seed development until shedding. Embryo elongation within seeds was investigated during seed development and under several temperature regimes after shedding. Key Results Seeds were shed at high moisture content (>59 %) with little evidence that dry mass accumulation or embryo elongation were complete. Ability to germinate developed prior to the ability of some seeds to tolerate enforced desiccation. Germination was sporadic and slow. Embryo elongation occurred post-shedding in moist environments, most rapidly at 20 °C in G. nivalis and 15 °C in N. pseudonarcissus. The greatest germination also occurred in these regimes, 78 and 48 %, respectively, after 700 d. Conclusions Seeds of G. nivalis and N. pseudonarcissus were comparatively immature at shedding and substantial embryo elongation occurred post-shedding. Seeds showed limited desiccation tolerance at dispersal. PMID:23478943

  5. In planta imaging of Δ9-tetrahydrocannabinolic acid in Cannabis sativa L. with hyperspectral coherent anti-Stokes Raman scattering microscopy

    NASA Astrophysics Data System (ADS)

    Garbacik, Erik T.; Korai, Roza P.; Frater, Eric H.; Korterik, Jeroen P.; Otto, Cees; Offerhaus, Herman L.

    2013-04-01

    Nature has developed many pathways to produce medicinal products of extraordinary potency and specificity with significantly higher efficiencies than current synthetic methods can achieve. Identification of these mechanisms and their precise locations within plants could substantially increase the yield of a number of natural pharmaceutics. We report label-free imaging of Δ9-tetrahydrocannabinolic acid (THCa) in Cannabis sativa L. using coherent anti-Stokes Raman scattering microscopy. In line with previous observations we find high concentrations of THCa in pistillate flowering bodies and relatively low amounts within flowering bracts. Surprisingly, we find differences in the local morphologies of the THCa-containing bodies: organelles within bracts are large, diffuse, and spheroidal, whereas in pistillate flowers they are generally compact, dense, and have heterogeneous structures. We have also identified two distinct vibrational signatures associated with THCa, both in pure crystalline form and within Cannabis plants; at present the exact natures of these spectra remain an open question.

  6. Somatic homologous recombination in planta: the recombination frequency is dependent on the allelic state of recombining sequences and may be influenced by genomic position effects.

    PubMed

    Swoboda, P; Hohn, B; Gal, S

    1993-02-01

    We have previously described a non-selective method for scoring somatic recombination in the genome of whole plants. The recombination substrate consists of a defective partial dimer of Cauliflower Mosaic Virus (CaMV) sequences, which can code for production of viable virus only upon homologous recombination; this leads to disease symptoms on leaves. Brassica napus plants (rapeseed) harbouring the recombination substrate as a transgene were used to examine the time in plant development at which recombination takes place. The analysis of three transgene loci revealed recombination frequencies specific for each locus. Recombination frequencies were increased if more than one transgene locus was present per genome, either in allelic (homozygosity of the transgene locus) or in non-allelic positions. In both cases, the overall recombination frequency was found to be elevated to approximately the sum of the frequencies for the individual transgene loci or slightly higher, suggesting that the respective transgene loci behave largely independently of each other. For all plants tested (single locus, two or multiple loci) maximal recombination frequencies were of the order of 10(-6) events per cell division.

  7. Phylogenetic Analysis of Downy Mildew Pathogens of Opium Poppy and PCR-Based In Planta and Seed Detection of Peronospora arborescens.

    PubMed

    Landa, Blanca B; Montes-Borrego, Miguel; Muñoz-Ledesma, Francisco J; Jiménez-Díaz, Rafael M

    2007-11-01

    ABSTRACT Severe downy mildew diseases of opium poppy (Papaver somniferum) can be caused by Peronospora arborescens and P. cristata, but differentiating between the two pathogens is difficult because they share morphological features and a similar host range. In Spain, where severe epidemics of downy mildew of opium poppy have occurred recently, the pathogen was identified as P. arborescens on the basis of morphological traits. In this current study, sequence homology and phylogenetic analyses of the internal transcribed spacer regions (ITS) of the ribosomal DNA (rDNA) were carried out with DNA from P. arborescens and P. cristata from diverse geographic origins, which suggested that only P. arborescens occurs in cultivated Papaver somniferum in Spain. Moreover, analyses of the rDNA ITS region from 27 samples of downy-mildew-affected tissues from all opium-poppy-growing regions in Spain showed that genetic diversity exists within P. arborescens populations in Spain and that these are phylogenetically distinct from P. cristata. P. cristata instead shares a more recent, common ancestor with a range of Peronospora species that includes those found on host plants that are not members of the Papaveraceae. Species-specific primers and a PCR assay protocol were developed that differentiated P. arborescens and P. cristata and proved useful for the detection of P. arborescens in symptomatic and asymptomatic opium poppy plant parts. Use of these primers demonstrated that P. arborescens can be transmitted in seeds and that commercial seed stocks collected from crops with high incidence of the disease were frequently infected. Field experiments conducted in microplots free from P. arborescens using seed stocks harvested from infected capsules further demonstrated that transmission from seedborne P. arborescens to opium poppy plants can occur. Therefore, the specific-PCR detection protocol developed in this study can be of use for epidemiological studies and diagnosing the pathogen in commercial seed stocks; thus facilitating the sanitary control of the disease and avoidance of the pathogen distribution in seeds.

  8. In planta protein interactions of three alphacryptoviruses and three betacryptoviruses from White Clover, Red Clover and Dill by bimolecular fluorescence complementation analysis.

    PubMed

    Lesker, Till; Maiss, Edgar

    2013-10-09

    Plant-infecting viruses of the genera Alpha- and Betacryptovirus within the family Partitiviridae cause no visible effects on their hosts and are only transmitted by cell division and through gametes. The bipartite dsRNA genome is encoding a RNA-dependent RNA polymerase (RdRp) and a coat protein (CP). Aside from sequence and structural analysis, the investigation of protein interactions is another step towards virus characterization. Therefore, ORFs of two type members White Clover Cryptic Virus 1 and 2 (WCCV-1 and WCCV-2), as well as the related viruses from Red Clover and Dill were introduced into a bimolecular fluorescence complementation assay. We showed CP-CP dimerization for all tested viruses with localization for alphacryptoviruses at the nuclear membrane and for betacryptoviruses close to cell walls within the cytoplasm. For CPs of WCCV-1 and WCCV-2, deletion mutants were created to determine internal interaction sites. Moreover, RdRp self-interaction was found for all viruses, whereas CP-RdRp interactions were only detectable for the alphacryptoviruses. An intra-genus test of CPs was successful in various virus combinations, whereas an inter-genus interaction of WCCV-1CP and WCCV-2CP was absent. This is the first report of in vivo protein interactions of members in the family Partitiviridae, indicating distinct features of the alpha- and betacryptoviruses.

  9. Investigations of the in Planta Expression of Active Cellobiohydrolase I: Cooperative Research and Development Final Report, CRADA Number CRD-07-219

    SciTech Connect

    Himmel, M.

    2011-02-01

    It was the purpose of this project to determine if cellulases produced in transgenic plants could effectively be utilized in the production of ethanol and other feedstock chemicals from lignocellulosic substrates.

  10. In planta processing and glycosylation of a nematode CLE effector and its interaction with a CLV2-like receptor to promote parasitism

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Like other biotrophic plant pathogens, plant-parasitic nematodes secrete effector proteins into host cells to facilitate infection. Effector proteins that mimic plant CLAVATA3/ESR (CLE)-like proteins have been identified in several cyst nematodes including the potato cyst nematode (PCN); however, th...

  11. AtSERPIN1 is an inhibitor of the metacaspase AtMC1-mediated cell death and autocatalytic processing in planta.

    PubMed

    Lema Asqui, Saul; Vercammen, Dominique; Serrano, Irene; Valls, Marc; Rivas, Susana; van Breusegem, Frank; Conlon, Frank L; Dangl, Jeffery L; Coll, Núria S

    2017-02-03

    The hypersensitive response (HR) is a localized programmed cell death phenomenon that occurs in response to pathogen recognition at the site of attempted invasion. Despite more than a century of research on HR, little is known about how it is so tightly regulated and how it can be contained spatially to a few cells. AtMC1 is an Arabidopsis thaliana plant metacaspase that positively regulates the HR. Here, we used an unbiased approach to identify new AtMC1 regulators. Immunoaffinity purification of AtMC1-containing complexes led us to the identification of the protease inhibitor AtSerpin1. Our data clearly showed that coimmunoprecipitation between AtMC1 and AtSerpin1 and formation of a complex between them was lost upon mutation of the AtMC1 catalytic site, and that the AtMC1 prodomain was not required for the interaction. AtSerpin1 blocked AtMC1 self-processing and inhibited AtMC1-mediated cell death. Our results constitute an in vivo example of a Serpin acting as a suicide inhibitor in plants, reminiscent of the activity of animal or viral serpins on immune/cell death regulators, including caspase-1. These results indicate a conserved function of a protease inhibitor on cell death regulators from different kingdoms with unrelated modes of action (i.e. caspases vs metacaspases).

  12. Beetle and plant arrow poisons of the Ju|'hoan and Hai||om San peoples of Namibia (Insecta, Coleoptera, Chrysomelidae; Plantae, Anacardiaceae, Apocynaceae, Burseraceae).

    PubMed

    Chaboo, Caroline S; Biesele, Megan; Hitchcock, Robert K; Weeks, Andrea

    2016-01-01

    The use of archery to hunt appears relatively late in human history. It is poorly understood but the application of poisons to arrows to increase lethality must have occurred shortly after developing bow hunting methods; these early multi-stage transitions represent cognitive shifts in human evolution. This paper is a synthesis of widely-scattered literature in anthropology, entomology, and chemistry, dealing with San ("Bushmen") arrow poisons. The term San (or Khoisan) covers many indigenous groups using so-called 'click languages' in southern Africa. Beetles are used for arrow poison by at least eight San groups and one non-San group. Fieldwork and interviews with Ju|'hoan and Hai||om hunters in Namibia revealed major differences in the nature and preparation of arrow poisons, bow and arrow construction, and poison antidote. Ju|'hoan hunters use leaf-beetle larvae of Diamphidia Gerstaecker and Polyclada Chevrolat (Chrysomelidae: Galerucinae: Alticini) collected from soil around the host plants Commiphora africana (A. Rich.) Engl. and Commiphora angolensis Engl. (Burseracaeae). In the Nyae Nyae area of Namibia, Ju|'hoan hunters use larvae of Diamphidia nigroornata Ståhl. Larvae and adults live above-ground on the plants and eat leaves, but the San collect the underground cocoons to extract the mature larvae. Larval hemolymph is mixed with saliva and applied to arrows. Hai||om hunters boil the milky plant sap of Adenium bohemianum Schinz (Apocynaceae) to reduce it to a thick paste that is applied to their arrows. The socio-cultural, historical, and ecological contexts of the various San groups may determine differences in the sources and preparation of poisons, bow and arrow technology, hunting behaviors, poison potency, and perhaps antidotes.

  13. Proteins with High Turnover Rate in Barley Leaves Estimated by Proteome Analysis Combined with in Planta Isotope Labeling1[W][OPEN

    PubMed Central

    Nelson, Clark J.; Alexova, Ralitza; Jacoby, Richard P.; Millar, A. Harvey

    2014-01-01

    Protein turnover is a key component in cellular homeostasis; however, there is little quantitative information on degradation kinetics for individual plant proteins. We have used 15N labeling of barley (Hordeum vulgare) plants and gas chromatography-mass spectrometry analysis of free amino acids and liquid chromatography-mass spectrometry analysis of proteins to track the enrichment of 15N into the amino acid pools in barley leaves and then into tryptic peptides derived from newly synthesized proteins. Using information on the rate of growth of barley leaves combined with the rate of degradation of 14N-labeled proteins, we calculate the turnover rates of 508 different proteins in barley and show that they vary by more than 100-fold. There was approximately a 9-h lag from label application until 15N incorporation could be reliably quantified in extracted peptides. Using this information and assuming constant translation rates for proteins during the time course, we were able to quantify degradation rates for several proteins that exhibit half-lives on the order of hours. Our workflow, involving a stringent series of mass spectrometry filtering steps, demonstrates that 15N labeling can be used for large-scale liquid chromatography-mass spectrometry studies of protein turnover in plants. We identify a series of abundant proteins in photosynthesis, photorespiration, and specific subunits of chlorophyll biosynthesis that turn over significantly more rapidly than the average protein involved in these processes. We also highlight a series of proteins that turn over as rapidly as the well-known D1 subunit of photosystem II. While these proteins need further verification for rapid degradation in vivo, they cluster in chlorophyll and thiamine biosynthesis. PMID:25082890

  14. Species-specific ITS primers for the identification of Picoa juniperi and Picoa lefebvrei and using nested-PCR for detection of P. juniperi in planta.

    PubMed

    Jamali, Samad; Banihashemi, Zia

    2013-10-01

    Desert truffles, hypogeous Pezizales (Ascomycota), are difficult to identify due to evolutionary convergence of morphological characters among taxa that share a similar habitat and mode of spore dispersal. Also, during their symbiotic phase, these are barely distinguishable morphologically, and molecular probes are needed for their identification. We have developed a PCR-based method for the identification of Picoa juniperi and Picoa lefebvrei based on internal transcribed spacers of rDNA. Two PCR primers specific for P. lefebvrei (FLE/RLE) and two specific for P. juniperi (FJU/RJU) were designed. A collection of samples from different geographical areas representing diversity of these species were examined for unique regions of internal transcribed spacers 1, 2 and 5.8S gene of rDNA (ITS) compared to other closely related species. Annealing temperatures and extension times were optimized for each set of primers for maximum specificity and efficiency. They proved to be efficient to specifically detect the presence of P. juniperi and P. lefebvrei by PCR and neither set amplified purified DNA from other truffle species as well as some ascomycetous fungi. The partial small subunit of ribosomal DNA genes of P. juniperi were amplified with the genomic DNA extracted from Helianthemum ledifolium var. ledifolium roots by nested polymerase chain reaction (PCR) using the universal fungal primer pair ITS1/ITS4 and specific primer pair FTC/RTC, which was designed based on internal transcribed spacer 1, 2 and 5.8S gene of rDNA sequences of P juniperi. The nested-PCR was sensitive enough to re-amplify the direct-PCR product, resulting in a DNA fragment of 426 bp. The efficacy of nested-PCR showed that it could re-amplify the direct-PCR product and detect 200 fg genomic DNA.

  15. In planta imaging of Δ⁹-tetrahydrocannabinolic acid in Cannabis sativa L. with hyperspectral coherent anti-Stokes Raman scattering microscopy.

    PubMed

    Garbacik, Erik T; Korai, Roza P; Frater, Eric H; Korterik, Jeroen P; Otto, Cees; Offerhaus, Herman L

    2013-04-01

    Nature has developed many pathways to produce medicinal products of extraordinary potency and specificity with significantly higher efficiencies than current synthetic methods can achieve. Identification of these mechanisms and their precise locations within plants could substantially increase the yield of a number of natural pharmaceutics. We report label-free imaging of Δ⁹-tetrahydrocannabinolic acid (THCa) in Cannabis sativa L. using coherent anti-Stokes Raman scattering microscopy. In line with previous observations we find high concentrations of THCa in pistillate flowering bodies and relatively low amounts within flowering bracts. Surprisingly, we find differences in the local morphologies of the THCa-containing bodies: organelles within bracts are large, diffuse, and spheroidal, whereas in pistillate flowers they are generally compact, dense, and have heterogeneous structures. We have also identified two distinct vibrational signatures associated with THCa, both in pure crystalline form and within Cannabis plants; at present the exact natures of these spectra remain an open question.

  16. The Pratylenchus penetrans Transcriptome as a Source for the Development of Alternative Control Strategies: Mining for Putative Genes Involved in Parasitism and Evaluation of in planta RNAi

    PubMed Central

    Vieira, Paulo; Eves-van den Akker, Sebastian; Verma, Ruchi; Wantoch, Sarah; Eisenback, Jonathan D.; Kamo, Kathryn

    2015-01-01

    The root lesion nematode Pratylenchus penetrans is considered one of the most economically important species within the genus. Host range studies have shown that nearly 400 plant species can be parasitized by this species. To obtain insight into the transcriptome of this migratory plant-parasitic nematode, we used Illumina mRNA sequencing analysis of a mixed population, as well as nematode reads detected in infected soybean roots 3 and 7 days after nematode infection. Over 140 million paired end reads were obtained for this species, and de novo assembly resulted in a total of 23,715 transcripts. Homology searches showed significant hit matches to 58% of the total number of transcripts using different protein and EST databases. In general, the transcriptome of P. penetrans follows common features reported for other root lesion nematode species. We also explored the efficacy of RNAi, delivered from the host, as a strategy to control P. penetrans, by targeted knock-down of selected nematode genes. Different comparisons were performed to identify putative nematode genes with a role in parasitism, resulting in the identification of transcripts with similarities to other nematode parasitism genes. Focusing on the predicted nematode secreted proteins found in this transcriptome, we observed specific members to be up-regulated at the early time points of infection. In the present study, we observed an enrichment of predicted secreted proteins along the early time points of parasitism by this species, with a significant number being pioneer candidate genes. A representative set of genes examined using RT-PCR confirms their expression during the host infection. The expression patterns of the different candidate genes raise the possibility that they might be involved in critical steps of P. penetrans parasitism. This analysis sheds light on the transcriptional changes that accompany plant infection by P. penetrans, and will aid in identifying potential gene targets for selection and use to design effective control strategies against root lesion nematodes. PMID:26658731

  17. Limited impact of abiotic stress on surfactin production in planta and on disease resistance induced by Bacillus amyloliquefaciens S499 in tomato and bean.

    PubMed

    Pertot, Ilaria; Puopolo, Gerardo; Hosni, Taha; Pedrotti, Lorenzo; Jourdan, Emmanuel; Ongena, Marc

    2013-12-01

    Understanding how temperature and water stress affect protocooperation between plants and beneficial rhizobacteria may enhance the efficacy of biocontrol agents in reducing plant diseases. However, little is known about the impact of these factors on biocontrol mechanisms and effectiveness, especially when provided by beneficial Bacillus spp. This work aimed to evaluate the influence of low/high temperature combined with a normal and reduced water regime on the interaction between Bacillus amyloliquefaciens strain S499 and plants, resulting in the induction of systemic resistance (ISR). A reduction in ISR level was observed when plants were subjected to stress before bacterization; however, root treatment with S499 prior to stress exposure attenuated this negative effect. Colonization of S499 during exposure to temperature/water stress allowed the three crops to conserve their overall ability to mount defense lines to a similar degree at all the temperatures tested. Further investigation revealed that relative production of surfactin by S499 was clearly enhanced at low temperature, making it possible to counter-balance the negative effect on traits associated with rhizosphere fitness (colonization, motility, and biofilm formation) observed in vitro in cold conditions. This work thus represents a first step in deciphering the effect of high/low temperatures and/or drought on key plant-microorganism interactions culminating in ISR.

  18. Heterologous overexpression and biochemical characterization of the (galactophospho)lipase from Fusarium solani in Pichia pastoris that is expressed in planta.

    PubMed

    Jallouli, Raida; Ali, Madiha Bou; Charfeddine, Mariam; Gargouri-Bouzid, Radhia; Gargouri, Youssef; Bezzine, Sofiane

    2016-03-01

    High-level extracellular production of Fusarium solani (galactophospho)lipase, named FSL, was achieved using a Pichia pastoris X33 expression system. The (galactophospho) lipase encoding gene was cloned into pGAPZαA with the Saccharomyces cerevisiae α-factor signal sequence by two different ways. The two constructs consist of an additional sequence of a (His)6-tag of the vector fused to the N-terminus of this enzyme (tFSL) while the other expression vector was constructed without any additional sequence (rFSL). Compared to the native enzyme (nFSL) (18.75 mg/L), a high level secretion of rFSL (310 mg/L) and tFSL (240 mg/L) was achieved providing an important improvement in enzyme production. Biochemical characterization showed that pure recombinant proteins (rFSL and tFSL) presented similar behaviour towards triglycerides, phospholipid and galactolipid. Like the nFSL, rFSL and tFSL are active at high concentration of bile salts (4mM) and calcium ions enhanced lipase activity. During plant infection, transcripts of this fungal lipase gene were detected 3, 7 and 10 days post infection.

  19. Characterisation of a new reporter system allowing high throughput in planta screening for recombination events before and after controlled DNA double strand break induction.

    PubMed

    Wehrkamp-Richter, Sophie; Degroote, Fabienne; Laffaire, Jean-Baptiste; Paul, Wyatt; Perez, Pascual; Picard, Georges

    2009-04-01

    DNA double strand breaks (DSBs) are created either by DNA damaging reagents or in a programmed manner, for example during meiosis. Homologous recombination (HR) can be used to repair DSBs, a process vital both for cell survival and for genetic rearrangement during meiosis. In order to easily quantify this mechanism, a new HR reporter gene that is suitable for the detection of rare recombination events in high-throughput screens was developed in Arabidopsis thaliana. This reporter, pPNP, is composed of two mutated Pat genes and has also one restriction site for the meganuclease I-SceI. A functional Pat gene can be reconstituted by an HR event giving plants which are resistant to the herbicide glufosinate. The basal frequency of intra-chromosomal recombination is very low (10(-5)) and can be strongly increased by the expression of I-SceI which creates a DSB. Expression of I-SceI under the control of the 35S CaMV promoter dramatically increases HR frequency (10,000 fold); however the measured recombinant events are in majority somatic. In contrast only germinal recombination events were measured when the meganuclease was expressed from a floral-specific promoter. Finally, the reporter was used to test a dexamethasone inducible I-SceI which could produce up to 200x more HR events after induction. This novel inducible I-SceI should be useful in fundamental studies of the mechanism of repair of DSBs and for biotechnological applications.

  20. Enhanced in planta Fitness through Adaptive Mutations in EfpR, a Dual Regulator of Virulence and Metabolic Functions in the Plant Pathogen Ralstonia solanacearum

    PubMed Central

    Rengel, David; Barlet, Xavier; Gouzy, Jérôme

    2016-01-01

    Experimental evolution of the plant pathogen Ralstonia solanacearum, where bacteria were maintained on plant lineages for more than 300 generations, revealed that several independent single mutations in the efpR gene from populations propagated on beans were associated with fitness gain on bean. In the present work, novel allelic efpR variants were isolated from populations propagated on other plant species, thus suggesting that mutations in efpR were not solely associated to a fitness gain on bean, but also on additional hosts. A transcriptomic profiling and phenotypic characterization of the efpR deleted mutant showed that EfpR acts as a global catabolic repressor, directly or indirectly down-regulating the expression of multiple metabolic pathways. EfpR also controls virulence traits such as exopolysaccharide production, swimming and twitching motilities and deletion of efpR leads to reduced virulence on tomato plants after soil drenching inoculation. We studied the impact of the single mutations that occurred in efpR during experimental evolution and found that these allelic mutants displayed phenotypic characteristics similar to the deletion mutant, although not behaving as complete loss-of-function mutants. These adaptive mutations therefore strongly affected the function of efpR, leading to an expanded metabolic versatility that should benefit to the evolved clones. Altogether, these results indicated that EfpR is a novel central player of the R. solanacearum virulence regulatory network. Independent mutations therefore appeared during experimental evolution in the evolved clones, on a crucial node of this network, to favor adaptation to host vascular tissues through regulatory and metabolic rewiring. PMID:27911943

  1. Beetle and plant arrow poisons of the Ju|’hoan and Hai||om San peoples of Namibia (Insecta, Coleoptera, Chrysomelidae; Plantae, Anacardiaceae, Apocynaceae, Burseraceae)

    PubMed Central

    Chaboo, Caroline S.; Biesele, Megan; Hitchcock, Robert K.; Weeks, Andrea

    2016-01-01

    Abstract The use of archery to hunt appears relatively late in human history. It is poorly understood but the application of poisons to arrows to increase lethality must have occurred shortly after developing bow hunting methods; these early multi-stage transitions represent cognitive shifts in human evolution. This paper is a synthesis of widely-scattered literature in anthropology, entomology, and chemistry, dealing with San (“Bushmen”) arrow poisons. The term San (or Khoisan) covers many indigenous groups using so-called ‘click languages’ in southern Africa. Beetles are used for arrow poison by at least eight San groups and one non-San group. Fieldwork and interviews with Ju|’hoan and Hai||om hunters in Namibia revealed major differences in the nature and preparation of arrow poisons, bow and arrow construction, and poison antidote. Ju|’hoan hunters use leaf-beetle larvae of Diamphidia Gerstaecker and Polyclada Chevrolat (Chrysomelidae: Galerucinae: Alticini) collected from soil around the host plants Commiphora africana (A. Rich.) Engl. and Commiphora angolensis Engl. (Burseracaeae). In the Nyae Nyae area of Namibia, Ju|’hoan hunters use larvae of Diamphidia nigroornata Ståhl. Larvae and adults live above-ground on the plants and eat leaves, but the San collect the underground cocoons to extract the mature larvae. Larval hemolymph is mixed with saliva and applied to arrows. Hai||om hunters boil the milky plant sap of Adenium bohemianum Schinz (Apocynaceae) to reduce it to a thick paste that is applied to their arrows. The socio-cultural, historical, and ecological contexts of the various San groups may determine differences in the sources and preparation of poisons, bow and arrow technology, hunting behaviors, poison potency, and perhaps antidotes. PMID:27006594

  2. Overview of Spanish and Latin American Distance Higher Education.

    ERIC Educational Resources Information Center

    Garcia Garrido, Jose Luis

    1991-01-01

    Provides a brief overview of Spanish and Latin American distance education programs for higher education and describes the three most important institutions: (1) the Spanish UNED (Universidad Nacional de Educacion a Distancia); (2) the Costa Rican UNED (Universidad Estatal a Distancia); and (3) the Venezuelan UNA (Universidad Nacional Abierta).…

  3. Velocidades radiales en Collinder 121

    NASA Astrophysics Data System (ADS)

    Arnal, M.; Morrell, N.

    Se han llevado a cabo observaciones espectroscópicas de unas treinta estrellas que son posibles miembros del cúmulo abierto Collinder 121. Las mismas fueron realizadas con el telescopio de 2.15m del Complejo Astronómico El Leoncito (CASLEO). El análisis de las velocidades radiales derivadas del material obtenido, confirma la realidad de Collinder 121, al menos desde el punto de vista cinemático. La velocidad radial baricentral (LSR) del cúmulo es de +17 ± 3 km.s-1. Esta velocidad coincide, dentro de los errores, con la velocidad radial (LSR) de la nebulosa anillo S308, la cual es de ~20 ± 10 km.s-1. Como S308 se encuentra físicamente asociada a la estrella Wolf-Rayet HD~50896, es muy probable que esta última sea un miembro de Collinder 121. Desde un punto de vista cinemático, la supergigante roja HD~50877 (K3Iab) también pertenecería a Collinder 121. Basándonos en la pertenencia de HD~50896 a Collinder 121, y en la interacción encontrada entre el viento de esta estrella y el medio interestelar circundante a la misma, se estima para este cúmulo una distancia del orden de 1 kpc.

  4. Apoyo a Estudios Geodinamicos con GPS en Guatemala

    NASA Astrophysics Data System (ADS)

    Robles, V. R.

    2013-05-01

    El Instituto Geografico Nacional de Guatemala implemento 17 estaciones GNSS en el año 2009, como un proyecto de credito mixto de donacion de equipamiento del Gobierno de Suiza, el cual, este equipamiento de estaciones CORS GNSS es un sistema de recepción y transmisión de datos crudos GPS RInex que utiliza la tecnologia Spider Web de Leica, asi mismo este sistema esta sirviendo para el espablecimiento de un marco geodesico nacional de coordenadas geodesicas oficiales, el cual se calculan u obtienen las velocidades en tiempos temporales programados de las 17 Estaciones CORS. La infraestructura del marco geodesico de Guatemala esta sirviendo de base para las aplicaciones de estudios geodinamicos como el monitoreo de del desplazamiento de las placas tectonicas por medio de un estudio que se inicio en el año de 1999, llamado medicion con GPS el sistema de Fallas de los rios Polochic Motagua de Guatemala, tambien para un estudio que se implemento para deformación de corteza terrestre local en un Volcan Activo de Guatemala llamado Pacaya. Para el estudio de medicion con GPS en el sistema de falla de los Rios del polochic Motagua se implementaron 16 puntos para medir con GPS de dos frecuencias en el año de 1999, el cual, tres puntos son estaciones geodesicas CORS IGS llamados GUAT, ELEN y HUEH, despues en el año de 2003 se hizo otra medicion en un total de 20 puntos, que permitió calcular las velocidades de desplazamieinto de los puntos en mención, usando como referencia el modelo NUVEL 1A de DeMets de la placa de Norteamerica. Este estudio fue en cooperación internacional por la universidad de Nice de Francia y el IGNde Francia. Para el estudio del monitoreo con GPS del volcan activo de Guatemala, se implementaron cuatro puntos al rededor del volcan, el cual, se realizan cuatro mediciones al año, que permiten determinar axialmente la distancias entre los puntos, y rebisar estadisticamente cual es el comportamiento de las distancias en funcion del tiempo, si

  5. Let's Talk About Water: Film Screenings as an Entrée to Water Science

    NASA Astrophysics Data System (ADS)

    Hooper, R. P.; Lilienfeld, L.; Arrigo, J.

    2011-12-01

    "Let's Talk about Water" is a film symposium designed to bring together experts and the public to talk about the complex water issues facing society. The format of the event is quite simple: a panel of experts and the audience view a water documentary (such as "FLOW", "Liquid Assets", or "Gasland") together and there is an extended moderated discussion period following the film between the panel and the audience. Properly handled, this simple format can be very effective. A film creates a context of subject and language for the discussion--it gets the audience and the panel on the same page. The moderators must actively manage the discussion, both challenging the panelists with follow up questions, asking questions to simplify the language the expert is using, and passing a question among panelists to bring out different points of view. The panelists are provided with the film in advance to view and, most importantly, meet the day before the event to discuss the film. This makes for a much more convivial discussion at the event. We have found that these discussions can easily be sustained for 90 to 120 minutes with active audience participation. This format has been applied at college campuses with a target audience of lower-level undergraduates. Student clubs are engaged to help with publicity before the event and to assist with registration and ushering during the event. Appropriate classes offer extra credit for student attendance to ensure a strong turnout. A Hollywood film ("Chinatown" in southern California, "A Civil Action" in Boston) is shown on campus during the week preceding the event to help advertise the event. The event itself is typically held on a Saturday with a morning screening of the film. The audience is provided with index cards and pencils to write down questions they have about the film. A lunch is provided during which the questions are organized and used to initiate different discussion themes. The discussion begins with points raised by the movie (are these issues real? Do they apply here? What are the scientific, engineering, and policy solutions to these problems?) and then segues into a discussion about career opportunities in the water sector. Our past events at UC Irvine and at UMass Boston have been successful in attracting large audiences and have been viewed positively by attendees.

  6. Actividades interrelacionadas entre el nino y la familia (Interrelated Activities between the Child and the Family).

    ERIC Educational Resources Information Center

    Veglio, Ana M.

    1993-01-01

    Offers a rationale for parent involvement in early childhood education. Describes the benefits of mother-child gymnastics in terms of the development of motor skills and positive attitudes toward school. Highlights particular exercises and games. (AC)

  7. Los Pactos entre Padres y Maestros (Teacher-Parent Partnerships). ERIC Digest.

    ERIC Educational Resources Information Center

    Swick, Kevin J.

    Research provides insight into parent attributes that support partnerships with teachers. These attributes include warmth, sensitivity, nurturance, the ability to listen, consistency, positive self-image, personal competence, and effective interpersonal skills. Researchers have cited positive attitudes, continuous teacher training, involvement in…

  8. GPS, punto de contacto entre la Astronomía y otras disciplinas

    NASA Astrophysics Data System (ADS)

    Perdomo, R.

    En los primeros años de la pasada década, un grupo de Astrónomos de La Plata iniciaron trabajos de investigación en Geodesia Satelital. En esta presentación se propone repasar sus principales logros y situarlos en el contexto del desarrollo de la disciplina a nivel mundial. Por entonces las aplicaciones prácticas de la disciplina eran indirectas y solo evidentes para los especialistas. Los errores del Posicionamiento Satelital eran de varios metros y en posicionamiento relativo, del orden de medio metro. Estos resultados se lograban al cabo de varios días de medición continuada. En los años siguientes, el sistema GPS alcanzó su nivel operacional y produjo una revolución tanto en lo relacionado con los tiempos de medición como con las precisiones. El grupo de La Plata se desarrolló simultáneamente con GPS, lo que posibilitó su participación actual en diversos temas vigentes: aplicaciones de alta precisión para la materialización de sistemas terrestres de referencia, monitoreo de movimientos de la corteza, contribución con servicios internacionales para la determinación de movimientos tectónicos globales y regionales, monitoreo de la ionósfera a partir de la propagación de las señales, determinación de la ondulación del geoide a escala local, etc. También se generaron muchas aplicaciones prácticas algunas en tiempo real: navegación, apoyo a imágenes aéreas o satelitales, aplicaciones a la agricultura, catastro y ordenamiento territorial, apoyo a relevamientos geofísicos, etc.

  9. Virtual Interfaces: Research and Application (Les Interfaces Virtuelles entre Recherche et Applications).

    DTIC Science & Technology

    1994-05-01

    donndes et de la d6tection des mouvements humains, alli6s aux mod6les informatis6s des milieux naturels et artificiels, ont conduit A la mise el pltace...d’interfaces dites virtuelles. Les interfaces virtuelles offrent plus de souplesse et de naturel et elles sont donc envisag6es pour les domaines tels...contr6leurs a6ricns. principes d𔄀tude du langage naturel . Basd sur l’utilisation concomnitante d’une interface 11 GENERALITES vocale (synth~se et

  10. La formation a Bukavu au Zaire: une course entre l'education et la catastrophe

    NASA Astrophysics Data System (ADS)

    Balegamire, Bazilashe Juvénal

    1996-11-01

    A description of the socio-educational situation in Bukavu since the colonial era clearly shows why the population has sought means of overcoming the gradual abandonment by the Zairean State of its responsibilities, which has been aggravated by the large-scale influx into Kivu, in Eastern Zaire, of refugees from Rwanda and Burundi and of Zaireans who has been living in those countries for some years. These means are principally trade occupations, small-time selling, short-term vocational training and higher and university education at private institutions which are financed locally and/or by funds from the North. A new approach has been deveoped, that of training the trainers working for non-governmental development organisations. Trainers of trainers have been recruited locally among the many graduates of the Higher Institutes of Education and Rural Development, which have been affected by unemployment and the miserable salaries which the State offers to its employees. The trainers of trainers could be trained by the Higher Institute of Rural Development, making the largest possible use of local skills. However, such training should be one of a set of reforms of the Zairean education system. All of this only makes sense if Zaire and its neighbours urgently defuse present socio-political tensions so that all of our populations can peacefully and resolutely engage in the healthy transformation of our societies.

  11. Vinculación entre varios cúmulos estelares y estructuras del medio interestelar

    NASA Astrophysics Data System (ADS)

    Corti, M. A.; Baume, G. L.; Panei, J. A.; Suad, L. A.; Testori, J. C.; Borissova, J.; Kurtev, R.; Chené, A. N.; Ramirez Alegría, S.

    2015-08-01

    We study the embedded clusters DBS77, 78, 102, 160, and 161 located in the Galactic plane in the fourth quadrant of the Milky Way and the surrounding interstellar medium (ISM). We analyzed UBVIc photometry (SOAR) and infrared spectroscopy (NTT, ESO). We complemented these data with JHK (VVV2MASS), HI 21 cm bands (SGPS), 1.4 GHz (ATCA), and 4.85 GHz (PMN). We did multiband analysis and spectral classification of the brightest stars in each area. We also identified the ISM structures possibly related to the clusters. Finally, we obtained the main parameters of the studied clusters, the structures of the ISM and the link between them.

  12. Entre Nous: A Tutorial Approach to the Teaching of Business French.

    ERIC Educational Resources Information Center

    Chaisson, Arthur P.

    Suffolk University (Massachusetts) has developed a degree program in international marketing in French and Spanish that links curricula in the economics department and the department of humanities and modern languages. Language tutorials are mandatory for students in the international economics major. The tutorials are taught by native French- and…

  13. L'Harmonisation des langues maliennes: entre l'Integration nationale et régionale

    NASA Astrophysics Data System (ADS)

    Ouane, Adama

    1991-03-01

    The standardization of Malian languages, which began in the period after independence, has been an educational reform guided more by linguists than by any institutional framework laid down by the political authorities of the country. This situation has permitted the technical application of a scientific strategy, but it has not led to widespread and general acceptance of the formulas which have been worked out. The consequent opposition between technical and political considerations, which have admittedly not always been in conflict, has been complicated by a set of additional factors which have made it difficult to reconcile internal (national) harmonization, dictated by local tendencies and balances between languages, and the need to respect the (regional) ecolinguistics of the country, whose linguistic and sociocultural divisions frequently extend well into other countries of the sub-region. This conflict is illustrated by the example of Malian languages with a wide regional distribution — Bamanan (Bambara) and Fulfulde (Fulani) — and by that of languages with a narrower distribution such as Bomu and Syenara-Mamara. The standardization of orthography and the creation of terminology in the MAPE (Mandingo-Peul) Project are the manifestation of a general acceptance of regionalism, while the choice of central, standard languages has often been made without regard for regional factors.

  14. New York: Les ecoles entre SURR et STAR (New York: Schools between SURR and STAR).

    ERIC Educational Resources Information Center

    Ueberschlag, Roger

    1994-01-01

    Three problems of New York City (New York) schools--overpopulation, low academic standards, violence--are examined, and an effort led by parent and teacher organizations to improve conditions is described. Threatened closings (schools under registration review, SURR) and a program of violence reduction (Straight Talk about Risks, STAR) are noted.…

  15. La Communicacion entre los Centros de Investigacion en Educacion (Communication among Educational Research Centers).

    ERIC Educational Resources Information Center

    Schiefelbein, Ernesto

    1972-01-01

    In Latin America there is a lack of communication concerning educational research. This lack has been underlined in many regional meetings, but no action has been taken. Possible steps that would lead to improvement include circulation of research summaries, both for completed and current works, efforts by research centers to organize meetings,…

  16. Dominicanas entre La Gran Manzana y Quisqueya: Family, Schooling, and Language Learning in a Transnational Context

    ERIC Educational Resources Information Center

    Rodriguez, Tracy

    2009-01-01

    Drawing from a one-year qualitative research study, this article explores the transnational lives and experiences of three young women and their little sisters in New York with close ties to the Dominican Republic. Using ethnographic research methods--life history interviews, focus groups, participant observation, and document analysis, I examine…

  17. Comparación entre los sitios de LLAMA y APEX

    NASA Astrophysics Data System (ADS)

    Bareilles, F. A.; Morras, R.; Hauscarriaga, F. P.; Guarrera, L.; Arnal, E. M.; Lepine, J. R. D.

    A comparison among the meteorological condition prevailing at the location of APEX (Atacama Pathfinder Experiment) telescope and the site selected to deploy LLAMA (Long Latin American Millimeter Array) is carried out. The later is dubbed Alto Chorrillo, and is located 4800 m above sea level and around 16 km eastward from the town of San Antonio de los Cobres (province of Salta). This work is part of a long term monitoring campaign aim at selecting sites for millimeter and submillimeter radioastronomy that is being carried out by the Instituto Argentino de Radioastronomía (IAR) since 2002. FULL TEXT IN SPANISH

  18. Estudio comparativo de cúmulos abiertos ricos en estrellas masivas

    NASA Astrophysics Data System (ADS)

    Baume, G.; Feinstein, A.; Vázquez, R. A.

    Los cúmulos abiertos muy jóvenes proveen valiosa información sobre las teorías de evolución estelar. No obstante, no se conoce mucho sobre los miembros mas débiles de estos objetos. Se llevaron a cabo entonces, estudios detallados (fotometría CCD-UBVI y polarimetría) en los cúmulos abiertos: Tr 14, Cr 272, Stok 16, NGC 6231, NGC 3293 y HM 1. Se realizó entonces un análisis comparativo entre ellos y con los objetos Pismis 20 (Vázquez et al. 1995) y NGC 5606 (Vázquez et al. 1994). En particular, se analizó la secuencia principal inferior de los distintos cúmulos, se estudió la extinción interestelar y las propiedades del polvo, se determinaron las correspondientes IMF, y se estudió la forma en que se distribuyen las estrellas menos masivas en relación con las más masivas. Como conclusiones del trabajo se puede decir que se han redeterminado con mayor precisión las distancias y las edades de los objetos bajo estudio, se han hallado varias estrellas con alta probabilidad de estar en fase de contracción y se ha notado una dispersión de edad entre las estrellas más brillantes y las menos brillantes (confirmando la idea de que la formación estelar no es simultánea ni independiente de la masa). Además, se han encontrado que las pendientes de las IMFs de estos objetos son más planas que la de las estrellas de campo y las de cúmulos más evolucionados, siendo comparable con las halladas en objetos jóvenes de la Nube Mayor de Magallanes (Will et al. 1996).

  19. Association between work shift and the practice of physical activity among workers of a poultry processing plant in Southern Brazil.

    PubMed

    da Silva Garcez, Anderson; Canuto, Raquel; Vieira Paniz, Vera Maria; Anselmo Olinto, Beatriz; Macagnan, Jamile; Liane Henn, Ruth; Pascoal Pattussi, Marcos; Anselmo Olinto, Maria Teresa

    2015-05-01

    Introducción: La práctica regular de actividad física (AF) se ha asociado con una mejor salud. Además, las características del trabajo se pueden determinar las conductas de la AF de los trabajadores, incluyendo el turno de trabajo. Sin embargo, relativamente pocos estudios han examinado el comportamiento AF entre los trabajadores por turnos. Objetivo: Este estudio tuvo como objetivo investigar la asociación entre turno de trabajo y la práctica de la AF entre los trabajadores de una planta de procesamiento de aves en el sur de Brasil. Métodos: Un estudio transversal se llevó a cabo con 1.206 trabajadores (786 mujeres), con edades entre 18 a 50 años, trabajando en turnos de una línea de producción que funciona las 24 horas/día. Los trabajadores que han participado en más de 150 minutos/semana de AF se consideraron activos. Se realizó un análisis multivariante mediante regresión de Poisson y todos los análisis se estratificó por sexo. Resultados: De los participantes en el estudio, el 36% (IC del 95%: 33-39) se consideraron activas y las características sociodemográficas asociadas con AF difirieron entre machos y hembras. En cuanto a los turnos de trabajo, los trabajadores del turno de noche tenían mayor prevalencia de la AF. Sin embargo, el aumento de la AF se asoció significativamente con el trabajo por turnos sobre todo entre las mujeres. Después de ajustar por factores de confusión, las mujeres que trabajaban en el turno de la noche fueron aproximadamente un 30% más activos que los que han trabajado durante el día (PR[razón de prevalencia]=1,32;IC del 95%: 1,07-1,62;p=0,010). Conclusiones: Este estudio encontró una baja prevalencia de PA entre los trabajadores e indicó una asociación significativa entre el trabajo por turnos y AF. Trabajar de noche se asoció positivamente con la AF regular, especialmente entre las mujeres. Estos resultados contribuyen a las iniciativas para aumentar la AF entre los trabajadores, teniendo en cuenta

  20. [BEHAVIOR OF HEMOGLOBIN CONCENTRATION, HEMATOCRIT AND OXYGEN SATURATION IN COLOMBIAN UNIVERSITY POPULATION AT DIFFERENT ALTITUDES].

    PubMed

    Trompetero González, Andrea Catalina; Cristancho Mejía, Edgar; Benavides Pinzón, William Fernando; Serrato Roa, Mauricio; Landinez Macias, María Paula; Rojas, Joel

    2015-11-01

    Introducción: el desarrollo de la presente investigación se basa en el creciente interés por comprender las adaptaciones a la hipoxia crónica, principalmente en el rango altitudinal denominado de alturas intermedias (1.500- 3.000 m.s.n.m), con la necesidad de establecer parámetros de normalidad en las variables [Hb], Hct y SO2 con fines diagnósticos y de caracterización de la población. Objetivos: analizar el comportamiento de la [Hb], el Hct y la SaO2 a diferentes alturas intermedias (970 m.s.n.m, 1.520 m.s.n.m, 1.728 m.s.n.m, 1.923 m.s.n.m, 2.180 m.s.n.m y 2.600 m.s.n.m) con el fin de aportar conocimiento sobre la fisiología de la altura y al campo clínico para apoyar el diagnóstico de anemia. Métodos: sujetos clínicamente sanos, con nivel bajo de actividad física y reporte de consumo de alimentos con contenido de hierro. Total de 264 participantes de ambos géneros entre 18 y 30 años. Las muestras se obtuvieron de sangre de la vena antecubital y del lóbulo de la oreja y fueron analizadas en un radiómetro. Se realizó un análisis estadístico no paramétrico. Resultados: con el incremento de la altitud los valores de [Hb] y Hct se incrementaron, mientras la SO2 descendió. Los hombres presentaron valores más altos que las mujeres en [Hb] y Hct, relacionado con valores más bajo de SO2 que las mujeres. Discusión: no se evidenció un umbral de las variables, quizás por la pequeña distancia entre las alturas. Los valores reportados fueron similares pero no iguales a otros estudios, lo que podría deberse a la diversidad genética entre poblaciones. Conclusiones: este estudio permite obtener los primeros valores de caracterización de la población estudiada. Todas las alturas estuvieron por encima del punto de corte para el diagnóstico de anemia ([Hb] 12 g/dl).

  1. Interplay of light and temperature during the in planta modulation of C4 phosphoenolpyruvate carboxylase from the leaves of Amaranthus hypochondriacus L.: diurnal and seasonal effects manifested at molecular levels.

    PubMed

    Avasthi, Uday K; Izui, Katsura; Raghavendra, Agepati S

    2011-01-01

    The interactive effects of light and temperature on C(4) phosphoenolpyruvate carboxylase (PEPC) were examined both in vivo and in situ using the leaves of Amaranthus hypochondriacus collected at different times during a day and in each month during the year. The maximum activity of PEPC, least inhibition by malate, and highest activation by glucose-6-phosphate were at 15.00 h during a typical day, in all the months. This peak was preceded by maximum ambient light but coincided with high temperature in the field. The highest magnitude in such responses was in the summer (e.g. May) and least in the winter (e.g. December). Light appeared to dominate in modulating the PEPC catalytic activity, whereas temperature had a strong influence on the regulatory properties, suggesting interesting molecular interactions. The molecular mechanisms involved in such interactive effects were determined by examining the PEPC protein/phosphorylation/mRNA levels. A marked diurnal rhythm could be seen in the PEPC protein levels and phosphorylation status during May (summer month). In contrast, only the phosphorylation status increased during the day in December (winter month). The mRNA peaks were not as strong as those of phosphorylation. Thus, the phosphorylation status and the protein levels of PEPC were crucial in modulating the daily and seasonal patterns in C(4) leaves in situ. This is the first detailed study on the diurnal as well as seasonal patterns in PEPC activity, its regulatory properties, protein levels, phosphorylation status, and mRNA levels, in relation to light and temperature intensities in the field.

  2. 454-pyrosequencing of Coffea arabica leaves infected by the rust fungus Hemileia vastatrix reveals in planta-expressed pathogen-secreted proteins and plant functions in a late compatible plant-rust interaction.

    PubMed

    Fernandez, Diana; Tisserant, Emilie; Talhinhas, Pedro; Azinheira, Helena; Vieira, Ana; Petitot, Anne-Sophie; Loureiro, Andreia; Poulain, Julie; Da Silva, Corinne; Silva, Maria Do Céu; Duplessis, Sébastien

    2012-01-01

    Coffee (Coffea arabica L.), one of the key export and cash crops in tropical and subtropical countries, suffers severe losses from the rust fungus Hemileia vastatrix. The transcriptome of H. vastatrix was analysed during a compatible interaction with coffee to obtain an exhaustive repertoire of the genes expressed during infection and to identify potential effector genes. Large-scale sequencing (454-GS-FLEX Titanium) of mixed coffee and rust cDNAs obtained from 21-day rust-infected leaves generated 352 146 sequences which assembled into 22 774 contigs. In the absence of any reference genomic sequences for Coffea or Hemileia, specific trinucleotide frequencies within expressed sequence tags (ESTs) and blast homology against a set of dicots and basidiomycete genomes were used to distinguish pathogen from plant sequences. About 30% (6763) of the contigs were assigned to H. vastatrix and 61% (13 951) to C. arabica. The majority (60%) of the rust sequences did not show homology to any genomic database, indicating that they were potential novel fungal genes. In silico analyses of the 6763 H. vastatrix contigs predicted 382 secreted proteins and identified homologues of the flax rust haustorially expressed secreted proteins (HESPs) and bean rust transferred protein 1 (RTP1). These rust candidate effectors showed conserved amino-acid domains and conserved patterns of cysteine positions suggestive of conserved functions during infection of host plants. Quantitative reverse transcription-polymerase chain reaction profiling of selected rust genes revealed dynamic expression patterns during the time course of infection of coffee leaves. This study provides the first valuable genomic resource for the agriculturally important plant pathogen H. vastatrix and the first comprehensive C. arabica EST dataset.

  3. In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends

    PubMed Central

    Fondevilla, Sara; Krezdorn, Nicolas; Rotter, Björn; Kahl, Guenter; Winter, Peter

    2015-01-01

    The most important foliar diseases in legumes worldwide are ascochyta blights. Up to now, in the Ascochyta-legume pathosystem most studies focused on the identification of resistance genes in the host, while very little is known about the pathogenicity factors of the fungal pathogen. Moreover, available data were often obtained from fungi growing under artificial conditions. Therefore, in this study we aimed at the identification of the pathogenicity factors of Ascochyta rabiei, causing ascochyta blight in chickpea. To identify potential fungal pathogenicity factors, we employed RNA-seq and Massive Analysis of cDNA Ends (MACE) to produce comprehensive expression profiles of A. rabiei genes isolated either from the fungus growing in absence of its host or from fungi infecting chickpea leaves. We further provide a comprehensive de novo assembly of the A. rabiei transcriptome comprising 22,725 contigs with an average length of 1178 bp. Since pathogenicity factors are usually secreted, we predicted the A. rabiei secretome, yielding 550 putatively secreted proteins. MACE identified 596 transcripts that were up-regulated during infection. An analysis of these genes identified a collection of candidate pathogenicity factors and unraveled the pathogen's strategy for infecting its host. PMID:26648917

  4. Visualization of the interaction between the precursors of VPg, the viral protein linked to the genome of turnip mosaic virus, and the translation eukaryotic initiation factor iso 4E in Planta.

    PubMed

    Beauchemin, Chantal; Boutet, Nathalie; Laliberté, Jean-François

    2007-01-01

    The RNA genome of Turnip mosaic virus is covalently linked at its 5' end to a viral protein known as VPg. This protein binds to the translation eukaryotic initiation factor iso 4E [eIF(iso)4E]. This interaction has been shown to be important for virus infection, although its exact biological function(s) has not been elucidated. In this study, we investigated the subcellular site of the VPg-eIF(iso)4E interaction using bimolecular fluorescence complementation (BiFC). As a first step, eIF(iso)4E, 6K-VPg-Pro, and VPg-Pro were expressed as full-length green fluorescent protein (GFP) fusions in Nicotiana benthamiana, and their subcellular localizations were visualized by confocal microscopy. eIF(iso)4E was predominantly associated with the endoplasmic reticulum (ER), and VPg-Pro was observed in the nucleus and possibly the nucleolus, while 6K-VPg-Pro-GFP induced the formation of cytoplasmic vesicles budding from the ER. In BiFC experiments, reconstituted green fluorescence was observed throughout the nucleus, with a preferential accumulation in subnuclear structures when the GFP split fragments were fused to VPg-Pro and eIF(iso)4E. On the other hand, the interaction of 6K-VPg-Pro with eIF(iso)4E was observed in cytoplasmic vesicles embedded in the ER. These data suggest that the association of VPg with the translation factor might be needed for two different functions, depending of the VPg precursor involved in the interaction. VPg-Pro interaction with eIF(iso)4E may be involved in perturbing normal cellular functions, while 6K-VPg-Pro interaction with the translation factor may be needed for viral RNA translation and/or replication.

  5. Sobre el estado evolutivo de β Pictoris

    NASA Astrophysics Data System (ADS)

    Brunini, A.; Benvenuto, O. G.

    Desde el descubrimiento de fuertes excesos infrarrojos en β Pictoris, esta estrella ha sido muy estudiada y es considerada candidata a poseer un sistema planetario propio. β Pic está rodeada de un disco asimétrico de polvo que se observa de canto y que esta vacío a distancias <= 40 AU. Esto se considera una fuerte evidencia en favor de la presencia de (al menos) un planeta gigante. Recientemente se han observado líneas de material circunestelar que se han interpretado como consecuencia de la caída de objetos cometarios sobre esta estrella. Recientemente se ha utilizado la existencia del disco de polvo para atribuir una edad corta (pre - secuencia principal) a βPic. Sin embargo, la evaporación de estos cometas provee suficiente polvo como para explicar la presencia del disco observado sin necesidad de edades cortas. En este trabajo mostramos que la comparación entre la tasa de impactos cometarios estimada en el Sistema Solar para diferentes etapas de su evolución y los datos observados en β Pic indica edades avanzadas para β Pic. Esta estimación debe tomarse con cautela ya que depende de la estructura de los sistemas planetarios. Además mostramos que, desde el punto de vista de la evolución estelar y con las incertezas presentes en la luminosidad y la temperatura efectiva, existe un continuo de edades posible para β Pic. Sin embargo, empleando los datos provenientes de los flujos cometarios encontramos que una edad prolongada es consistente con ambos tratamientos.

  6. Movimientos peculiares de galaxias en el Universo cercano

    NASA Astrophysics Data System (ADS)

    Alonso, M. V.

    Se presenta un resumen de los resultados más importantes obtenidos en los últimos años sobre movimientos peculiares en el Universo Cercano. En el escenario de inestabilidad gravitacional, el campo de velocidades peculiares es una herramienta muy útil para estimar las fluctuaciones en la distribución de masa que generan los movimientos observados. Esta aproximación puede, además, ser utilizada para poner restricciones al espectro de potencia de la masa, a la relación entre las distribuciones de galaxias y de materia y al valor del parámetro de densidad cosmológico, Ω, a grandes escalas. Son además presentados una reconstrucción preliminar de los campos de densidad de masa y velocidades peculiares en el universo cercano usando nuevas medidas de distancias obtenidas con la relación Dn - σ, para una muestra de galaxias elípticas y lenticulares. Dos subestructuras prominentes son encontradas en la región del Gran Atractor, que corresponden a los complejos de Centaurus y Pavo--Indus. Estos últimos, junto a los complejos de Perseus--Piscis y Cetus parecen ser importantes estructuras que determinan el flujo global. El patrón obtenido a través de una muestra de galaxias elípticas muestra variaciones con respecto al obtenido por galaxias espirales. Estos nuevos resultados muestran una coherencia menor en el flujo global, cuando son comparados con resultados obtenidos anteriormente.

  7. Metonymie dans la presse ecrite: entre discours et langue (Metonymies in the Press: Between Discourse and Language).

    ERIC Educational Resources Information Center

    Lecolle, Michelle

    2001-01-01

    This paper describes certain metonymies that are often employed in the daily French press. In such metonymies, a human being or a set of individuals are referred to by means of the name of an institutional location (i.e., a capital city, a ministry in a western country, the name of a country). These metonymic patterns do not seem to be used by…

  8. Interactivite, relations entre interlocuteurs et constitution d'unites conversationnelles (Interaction Processes, Participants' Relationships and Definition of Conversation Units).

    ERIC Educational Resources Information Center

    Zenone, Anna

    1981-01-01

    Analyzes a type of conversational exchange where the participants focus on a given subject or theme contributing personal views to the discussion. Characterizes the relationship among the participants as a "cooperative conflict," examining the internal structure of speech acts and their illocutory functions, particularly the initiatory…

  9. Estudio de la interacción entre Tit·n y la magnetósfera de Saturno

    NASA Astrophysics Data System (ADS)

    Villarreal D'Angelo, C. S.; Caranti, G. M.

    In this work we present a study of the interaction between Titan and the plasma in Saturn magnetosphere from the data obtained with the magne- tometer on board Cassini during one flyby to the saturnian moon in 2009. The analisys of the data was divided in two parts, one corresponding to the entire trajectory and the other corresponding to a few hours around closest approach. Also, an MHD code was utilized to reproduce the observation. FULL TEXT IN SPANISH

  10. Entre el Renacimiento y el Nuevo Mundo: Vida y obras de Hernan Perez de Oliva (1494?-1531)

    ERIC Educational Resources Information Center

    Pellus Perez, Elena

    2012-01-01

    Hernan Perez de Oliva (1494?-1531) was a learned Spanish humanist who wrote his major works in the decades following the discovery of America. By articulating events of his career and literary life, my goal is to comprehend his contributions to the early literary production regarding the newly-found Americas. The thesis consists of three parts.…

  11. Investigation des correlations existant entre la perception de qualite audio et les reactions physiologiques d'un auditeur

    NASA Astrophysics Data System (ADS)

    Baudot, Matthias

    Les tests d'ecoute subjectifs permettent d'evaluer la fiabilite de reproduction des systemes de codage audio (codecs). Le projet presente ici vise a evaluer la possibilite d'utiliser les reactions physiologiques (activite electrodermale, cardiaque, musculaire et cerebrale) a la place d'une note donnee par l'auditeur, afin de caracteriser la performance d'un codec. Ceci permettrait d'avoir une methode d'evaluation plus proche de la perception reelle de qualite audio du sujet. Des tests d'ecoute mettant en oeuvre des degradations audio bien connues en concours avec la mesure des reactions physiologiques ont ete realises pour 4 auditeurs. L'analyse des resultats montre que certaines caracteristiques physiologiques permettent d'avoir une information fiable sur la qualite audio percue, et ce de maniere repetable pour pres de 70% des signaux audio testes chez un sujet, et pres de 60% des sequences audio testees chez tous les sujets. Ceci permet de postuler sur la faisabilite d'une telle methode d'evaluation subjective des codecs audio. Mots-cles : test d'ecoute subjectif, evaluation des codecs audio, mesures physiologiques, qualite audio percue, conductance electrodermale, photoplethysmographie, electromyogramme, electroencephalogramme

  12. Detection of PhIP in grilled chicken entrées at popular chain restaurants throughout California.

    PubMed

    Sullivan, Kristie M; Erickson, Michael A; Sandusky, Chad B; Barnard, Neal D

    2008-01-01

    Heterocyclic amines (HCAs), compounds formed when meat is cooked at high temperatures particularly through pan frying, grilling, or barbequing, pose a potential carcinogenic risk to the public. It is unclear whether there is any level at which consumption of HCAs can be considered safe. Efforts to measure these compounds mainly include cooking studies under laboratory conditions and some measurement of home-cooked foods, but analysis of commercially cooked foods has been minimal. Attempts to estimate exposure of the public to these compounds must take into consideration dining outside the home, which could result in significant exposure for some individuals. We surveyed at least 9 locations each of 7 popular chain restaurants (McDonald's, Burger King, Chick-fil-A, Chili's, TGI Friday's, Outback Steakhouse, and Applebee's) in California, collecting one or two entrees from each location. Entrees were analyzed for 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) using high-performance liquid chromatography tandem mass spectrometry. All 100 samples contained PhIP. Concentrations were variable within and between entrees and ranged from 0.08 to 43.2 ng/g. When factoring in the weight of the entrees, absolute levels of PhIP reached over 1,000 ng for some entrees. Potential strategies for reducing exposure include the avoidance of meats cooked using methods that are known to form PhIP.

  13. Relación entre distribuciones de colisiones de baja energía y trayectorias de escape

    NASA Astrophysics Data System (ADS)

    Zoppetti, F. A.; Leiva, A. M.; Briozzo, C. B.

    In the restricted three-body problem; the impact distributions on the secondary mass originated by low-energy trajectories exhibit peculiar properties concerning the positions and directions of the impacts. In particular; for low energies there are regions on the surface of the secondary mass where no collisions happen. On the other hand; similar surface distributions can be obtained when only considering those test particles whose low-energy trajectories satisfy the escape condition. In this work; taking into account the Earth-Moon problem; we analyze the relations between these two distributions and discuss their possible applications. FULL TEXT IN SPANISH

  14. Relation entre la composition des smectites d'alteration sous-marine et leur cortege de terres rares

    NASA Astrophysics Data System (ADS)

    Desprairies, Alain; Bonnot-Courtois, Chantal

    1980-06-01

    In basalts and volcanogenic sediments from the Indian Ocean, the successive stages of submarine alteration of volcanic rocks and glasses give rise to the incorporation or the relative increase of iron in smectite lattices. During the first stage, the Mg-smectites are the most abundant; they are occasionally associated with Al-smectites. Afterwards, they are gradually replaced by iron-rich smectites. The REE distribution follows the same trend as the mineralogical changes. During the first stage of alteration, REE distribution in clay minerals is the same as in the fresh glasses but, when the iron-rich smectites increase, the Ce has a specific behaviour. The Ce shows a positive anomaly in iron-rich smectites formed early in palagonitized glasses, and a negative one in authigenic smectites formed later from solutions in equilibrium with seawater.

  15. Outbreak of Paratyphoid Fever Among Naval Personnel in Peru (Brote de Fiebre Paratifoidea Entre Personal de la Marina Del Peru.)

    DTIC Science & Technology

    1992-01-01

    de ausencia la- Rp = propore6n de ]a poblacion que res- borall per enfermedad y los consiguientes ponimal cuestienano = R/P = 0,653 gastos en atena6n...medium for control . N b, 1/M’d. ,70;283:N%-73’). shipment of clinical sp cimens: i, fecal speci- mens. I Bactorii. 1964;88.%-98. 4. Huckstep RL Ty#Aoi *vi...1991: rence to salmonella: its epIdemkiog., pathoge- 3-.w nvsis and control . Clin ptGrtnet, . 1979;8:863. D Iulbwl 11 l., I Ikernng I.K. jfwvtrw ; i

  16. Alternancia entre el estado de emisión de Rayos-X y Pulsar en Sistemas Binarios Interactuantes

    NASA Astrophysics Data System (ADS)

    De Vito, M. A.; Benvenuto, O. G.; Horvath, J. E.

    2015-08-01

    Redbacks belong to the family of binary systems in which one of the components is a pulsar. Recent observations show redbacks that have switched their state from pulsar - low mass companion (where the accretion of material over the pulsar has ceased) to low mass X-ray binary system (where emission is produced by the mass accretion on the pulsar), or inversely. The irradiation effect included in our models leads to cyclic mass transfer episodes, which allow close binary systems to switch between one state to other. We apply our results to the case of PSR J1723-2837, and discuss the need to include new ingredients in our code of binary evolution to describe the observed state transitions.

  17. [Effects of branched amino acids in endurance sports: a review].

    PubMed

    Salinas-García, María Elia; Martínez-Sanz, José Miguel; Urdampilleta, Aritz; Mielgo-Ayuso, Juan; Norte Navarro, Aurora; Ortiz-Moncada, Rocio

    2014-11-16

    Introducción: El informe emitido por la Agencia Europea de Seguridad Alimentaria (EFSA) en 2010 sobre las declaraciones nutricionales y propiedades saludables, muestra que no existen evidencias científicas que apoyen la suplementación con aminoácidos ramificados (BCAAs). El objetivo de este estudio es analizar los efectos del consumo de suplementos de BCAAs en deportes de larga duración (DLD). Métodos: Estudio descriptivo de revisión bibliográfica sobre el estado actual del efecto del consumo de suplementos de BCAAs. Se realizó una búsqueda en la base de datos PubMed y estrategia de bola de nieve. Criterios de inclusión: Estudios realizados en humanos, ensayos clínicos controlados aleatorizados (ECCA) en castellano/inglés relacionados con el consumo de BCAAs, leucina, valina e isoleucina en DLD y sus efectos sobre el daño muscular, rendimiento deportivo, fatiga central, respuesta anabólica y sistema inmunológico publicados en cualquier país hasta mayo 2014. Resultados: De los 330 estudios identificados, 14 cumplieron los criterios de inclusión. La media de sujetos participantes en los estudio es igual a (11,36 ± 7,43). Sólo dos estudios incluyen un grupo de mujeres. Las disciplinas deportivas que se encontraron en los estudios fueron carrera a pie, ciclismo, combinación ciclismo y carrera a pie, triatlón distancia olímpica y un estudio que incluía 2 grupos de deportistas (triatlón distancia olímpica y carrera a pie). Se estudian los efectos de los BCAAs y daño muscular, rendimiento deportivo, fatiga central, respuesta anabólica en periodo de recuperación y respuesta inmunológica en periodos diferentes del entrenamiento: antes, durante y después o una combinación de éstos. Discusión: Se observa que existe un menor grado de dolor y daño muscular, menor percepción del esfuerzo y fatiga mental, mayor respuesta anabólica en periodo de recuperación y mejora de la respuesta inmunológica cuando se suplementa con BCAAs, no obstante su

  18. Diet of a sigmodontine rodent assemblage in a Peruvian montane forest.

    PubMed

    Sahley, Catherine Teresa; Cervantes, Klauss; Pacheco, Victor; Salas, Edith; Paredes, Diego; Alonso, Alfonso

    2015-09-29

    comportamiento de forrajeo en roedores, aún existen grandes vacíos en nuestra comprensión de sus gremios tróficos. Nuestro objetivo fue investigar las dietas de siete especies de pequeños (< 100 g) roedores sigmodontinos simpátricos en un bosque montano andino a 3.500] m en Perú. Cualitativamente y cuantitativamente evaluamos la dieta en muestras fecales de roedores capturados entre el 2009 y el 2012. Datos de frecuencia para cuatro categorías de dieta indicaron que las siete especies de roedores consumieron cuatro categorías de dieta: artrópodos (88%), pedazos de hojas y fibras de plantas (61%), semillas intactas (con o sin pulpa de frutos; 50%), y esporas de micorrizas (45%). Omnivoría fue la estrategia utilizada por todas las especies, aunque el análisis con tablas de contingencia reveló diferencias significativas entre y dentro de especies en categorías de dieta. El análisis de agrupación presentó 2 grupos principales: el grupo Thomasomys spp. y Calomys sorellus, que incluye una gran proporción de semillas intactas, y partes de plantas en las muestras fecales y el grupo que incluye los géneros Akodon, Microryzomys y Oligoryzomys, el cual incluyó una proporción mayor de artrópodos en sus muestras fecales, pero con niveles altos de semillas intactas. Semillas intactas de al menos 17 especies de plantas (9 familias) fueron encontradas en las muestras fecales. Concluimos que este ensamble de roedores sigmodontinos es omnívoro y que probablemente las especies juegan un rol importante como frugívoros y en la dispersión de semillas en los bosques montanos tropicales en el Perú.

  19. Diet of a sigmodontine rodent assemblage in a Peruvian montane forest

    PubMed Central

    Sahley, Catherine Teresa; Cervantes, Klauss; Pacheco, Victor; Salas, Edith; Paredes, Diego; Alonso, Alfonso

    2015-01-01

    comportamiento de forrajeo en roedores, aún existen grandes vacíos en nuestra comprensión de sus gremios tróficos. Nuestro objetivo fue investigar las dietas de siete especies de pequeños (< 100 g) roedores sigmodontinos simpátricos en un bosque montano andino a 3.500] m en Perú. Cualitativamente y cuantitativamente evaluamos la dieta en muestras fecales de roedores capturados entre el 2009 y el 2012. Datos de frecuencia para cuatro categorías de dieta indicaron que las siete especies de roedores consumieron cuatro categorías de dieta: artrópodos (88%), pedazos de hojas y fibras de plantas (61%), semillas intactas (con o sin pulpa de frutos; 50%), y esporas de micorrizas (45%). Omnivoría fue la estrategia utilizada por todas las especies, aunque el análisis con tablas de contingencia reveló diferencias significativas entre y dentro de especies en categorías de dieta. El análisis de agrupación presentó 2 grupos principales: el grupo Thomasomys spp. y Calomys sorellus, que incluye una gran proporción de semillas intactas, y partes de plantas en las muestras fecales y el grupo que incluye los géneros Akodon, Microryzomys y Oligoryzomys, el cual incluyó una proporción mayor de artrópodos en sus muestras fecales, pero con niveles altos de semillas intactas. Semillas intactas de al menos 17 especies de plantas (9 familias) fueron encontradas en las muestras fecales. Concluimos que este ensamble de roedores sigmodontinos es omnívoro y que probablemente las especies juegan un rol importante como frugívoros y en la dispersión de semillas en los bosques montanos tropicales en el Perú. PMID:26937050

  20. Prediction uncertainty of plume characteristics derived from a small number of measuring points

    NASA Astrophysics Data System (ADS)

    French, H. K.; van der Zee, S. E. A. T. M.; Leijnse, A.

    sensibles à la dimension du panache en fonction de la distance entre les différents points de mesure. Afin de réduire les erreurs de prédiction de la variance, la distance entre les points de mesure doit être inférieure au double de l'écart-type du panache examiné. La dimension totale de l'ensemble doit couvrir une étendue de plusieurs écarts-types du panache pour éviter qu'une partie de la matière échappe à la zone surveillée. Des simulations numériques du panache en dispersion (les calculs de comparaison sont basés sur 9000 nœuds avec 30 points de mesure) montrent que le centre vertical et le centre horizontal de la matière dispersée ont été bien prédits à tous les degrés d'hétérogénéité, de même que pour les variances horizontales. Les variances verticales ont été plus sensibles aux erreurs de prédiction, mais les estimations étaient du même ordre de grandeur que les valeurs réelles. Resumen Un número pequeño de puntos de medida puede producir un sesgo en la caracterización en campo del flujo y transporte de solutos en la zona no saturada. La simulación de transporte advectivo (no difusivo) de un penacho Gaussiano a travs de un conjunto de 30 puntos de medida regularmente distribuidos revelan fluctuaciones temporales regulares de los momentos espaciales del penacho. Una distribución irregular de puntos de medida predijo a su vez fluctuaciones irregulares, más alejadas de la realidad, por lo que se recomienda el uso de esquemas de muestreo regulares. Los momentos espaciales fueron sensibles a la relación entre tamaño del penacho y distancia entre puntos de medida. Para reducir los errores en la predicción de la varianza, la distancia entre puntos de observación debe ser menor que dos veces la desviación estándar del penacho. El tamaño del área muestreada debe cubrir varias desviaciones estándar del penacho para evitar perder parte de la masa. Las simulaciones numricas en un penacho dispersivo, comparando los c

  1. Medicinal ethnobotany in Huacareta (Chuquisaca, Bolivia)

    PubMed Central

    2012-01-01

    used as a base for subsequent work related to traditional medicine and its contribution to allopathic medicine in San Pablo de Huacareta. Resumen Introducción El objetivo del presente estudio fue documentar los tipos de enfermedades tratadas mediante el uso de plantas medicinales, sus aplicaciones principales y también tener un reporte de las enfermedades mayormente atendidas en el Hospital de San Pablo de Huacareta (Chuquisaca, Bolivia). Métodos Se realizaron encuestas semiestructuradas a 10 informantes locales anotando los usos atribuidos a sus plantas medicinales, se agruparon las plantas por categorías de enfermedades tratadas en la medicina tradicional. Se obtuvieron reportes de casos tratados en el Hospital de Huacareta para poder relacionar el tratamiento de enfermedades recurrentes en la zona entre la medicina tradicional y la medicina occidental. Resultados Se reportaron 91 especies nativas y exóticas, además de un espécimen indeterminado exótico que intervienen en un total de 258 aplicaciones medicinales, las cuales son empleadas en un total de 13 categorías de enfermedades. Los desórdenes gastrointestinales (55%) son mayormente tratados mediante plantas medicinales, seguidas de las afecciones al sistema esqueleto-muscular (25%) y enfermedades dermatológicas (24%). La información del Hospital indica que las enfermedades más frecuentes son Infecciones Respiratorias Agudas (47%) y Enfermedades Diarreicas Agudas (37%). Los remedios vegetales se emplean en forma de infusiones y cocciones principalmente. Se emplean mayormente plantas nativas, también se introdujo en la farmacopea médica el uso de plantas exóticas al lugar. Conclusiones El tratamiento de trastornos gastrointestinales constituye el objetivo primordial de la etnobotánica médica de los habitantes de Huacareta, las enfermedades del sistema respiratorio, son mayormente tratadas en el Hospital. Observando los datos del libro de consultas del Hospital, se puede inferir que los des

  2. Parent-adolescent communication in foster, inter-country adoptive, and biological Italian families: Gender and generational differences.

    PubMed

    Rosnati, Rosa; Iafrate, Raffaella; Scabini, Eugenia

    2007-02-01

    . En tanto que los adolescentes adoptados perciben una comunicación más positiva que la de sus compañeros que viven en familias biológicas y de cuidado temporal. En el nivel de díadas, surgieron algunas diferencias entre los tres grupos. En las familias biológicas, se observó una distancia más pronunciada entre padres e hijos. En las familias adoptivas, el padre y el adolescente comparten percepciones más similares, mientras que discrepan significativamente madre e hijo. Se encontró un nivel mayor de congruencia perceptual entre los adolescentes y sus padres en las familias de cuidado temporal. En cuanto a diferencias de género, las madres experimentan una comunicación más abierta con sus hijos en comparación con los padres, y los adolescentes, sobretodo las mujeres, se comunican mejor con sus madres que con sus padres en los tres grupos de familias.

  3. [THE EFFECT OF A HAMSTRING STRECHING PROTOCOL ON HAMSTRING EXTENSIBIITY IN ADOLESCENT STUDENTS: INFLUENCE OF THE CLASS SESSION WEEKLY DISTRIBUTION].

    PubMed

    Bohajar-Lax, Álvaro; Vaquero-Cristóbal, Raquel; Espejo-Antúnez, Luis; López-Miñarro, Pedro Ángel

    2015-09-01

    Introducción: la realización de estiramientos de la musculatura isquiosural, dos sesiones por semana en días alternos, produce mejoras significativas en escolares. Objetivo: comparar los efectos de un programa de estiramientos de la musculatura isquiosural realizado en las clases de educación física en dos grupos de adolescentes, en función de la distribución semanal de sus sesiones de clase (sesiones alternas vs. sesiones consecutivas). Metodología: cincuenta y ocho estudiantes adolescentes realizaron un programa de estiramientos estático- activos de la musculatura isquiosural durante cinco semanas, ejecutados al comienzo de dos clases semanales de educación física, con una duración total de cinco minutos. Los alumnos fueron divididos en dos grupos: el grupo A, formado por 30 alumnos, tenían las clases en días consecutivos (lunes-martes) y el grupo B, compuesto por 29 alumnos, en días no consecutivos (martes-viernes). La extensibilidad de la musculatura isquiosural se evalúo mediante el test sit-and-reach antes del inicio del programa (pre-test) y tras finalizar el mismo (post-test). Resultados y discusión: se encontró una mejora significativa de la distancia alcanzada en el test sit-and-reach tanto en el grupo A (+2,15 cm) (p = 0,003) como en el grupo B (+2,31 cm) (p < 0,001). No se encontraron diferencias significativas ni en el pre-test ni en el post-test entre grupos (p > 0,05). Conclusiones: la aplicación de un programa de intervención con una duración de cinco semanas y una frecuencia de dos sesiones semanales produce mejoras en la extensibilidad isquiosural en escolares. La distribución semanal de las sesiones no influye en los resultados encontrados.

  4. Planificación Neuroquirúrgica con Software Osirix

    PubMed Central

    Jaimovich, Sebastián Gastón; Guevara, Martin; Pampin, Sergio; Jaimovich, Roberto; Gardella, Javier Luis

    2014-01-01

    Introducción: La individualidad anatómica es clave para reducir el trauma quirúrgico y obtener un mejor resultado. Actualmente, el avance en las neuroimágenes ha permitido objetivar esa individualidad anatómica, permitiendo planificar la intervención quirúrgica. Con este objetivo, presentamos nuestra experiencia con el software Osirix. Descripción de la técnica: Se presentan 3 casos ejemplificadores de 40 realizados. Caso 1: Paciente con meningioma de la convexidad parasagital izquierda en área premotora; Caso 2: Paciente con macroadenoma hipofisario, operada previamente por vía transeptoesfenoidal en otra institución con una resección parcial; Caso 3: Paciente con lesiones en pedúnculo cerebeloso medio bilateral. Se realizó la planificación prequirúrgica con el software OsiriX, fusionando y reconstruyendo en 3D las imágenes de TC e IRM, para analizar relaciones anatómicas, medir distancias, coordenadas y trayectorias, entre otras funciones. Discusión: El software OsiriX de acceso libre y gratuito permite al cirujano, mediante la fusión y reconstrucción en 3D de imágenes, analizar la anatomía individual del paciente y planificar de forma rápida, simple, segura y económica cirugías de alta complejidad. En el Caso 1 se pudo analizar las relaciones del tumor con las estructuras adyacentes para minimizar el abordaje. En el Caso 2 permitió comprender la anatomía post-operatoria previa del paciente, para determinar la trayectoria del abordaje transnasal endoscópico y la necesidad de ampliar su exposición, logrando la resección tumoral completa. En el Caso 3 permitió obtener las coordenadas estereotáxicas y trayectoria de una lesión sin representación tomográfica. Conclusión: En casos de no contar con costosos sistemas de neuronavegación o estereotáxia el software OsiriX es una alternativa a la hora de planificar la cirugía, con el objetivo de disminuir el trauma y la morbilidad operatoria. PMID:25165617

  5. Acute physiological response to indoor cycling with and without hydration; case and self-control study.

    PubMed

    Ramos-Jiménez, A; Hernández-Torres, R P; Wall-Medrano, A; Torres-Durán, P V; Juárez-Oropeza, M A; Solis Ceballos, J A

    2013-01-01

    Introducción: Las bebidas de re-hidratación oral ayudan a mantener la capacidad física y el nivel de hidratación durante el ejercicio. Objetivo: Evaluar en un estudio de casos y autocontroles la efectividad de tres protocolos de hidratación y ejercicio sobre la capacidad de trabajo, estrés físico y psicosomático durante el ciclismo de sala (CiS). Métodos: 14 varones eutróficos de mediana edad participaron de manera aleatoria y no consecutiva en tres protocolos de hidratación controlada (~278 mL 6/c 15 min) y ejercicio (CiS/90 min): No líquidos, agua corriente o bebida deportiva (BD). Las variables respuesta fueron: Temperatura corporal (TC), frecuencia cardiaca (FC) y presión sanguínea media (PSM). Las covariables: distancia recorrida (DR), resistencia del ergómetro (R) grasa corporal (GC), pérdida de peso al final del ejercicio ( PC) y edad de los participantes. Las diferencias entre los protocolos fueron evaluadas por GLM de Medidas Repetidas, la independencia de las asociaciones por regresión lineal múltiple. Resultados: En no líquidos, los sujetos presentaron mayor TC, FC y PSM que cuando ingirieron agua corriente o BD (p < 0,01). La capacidad de trabajo fue igual en los tres protocolos de hidratación. La TC fue la variable más sensible que detectó el estado de hidratación de los sujetos. El 34%, 99% y 21% de la varianza asociada a FC, PSM y TC lo explicaron DR + TC, TC + GC, y ?PC + edad + R + DR + GC respectivamente. Conclusiones: La ingesta de líquidos con o sin electrolitos no afecta la capacidad de trabajo y son igualmente efectivos como medios de hidratación durante =?90 min de Cis a intensidades pesadas y muy pesadas. La TC es la variable más sensible que detecta el estado de hidratación de los sujetos durante el ejercicio.

  6. Tools for evaluating Lipolexis oregmae (Hymenoptera: Aphidiidae) in the field: Effects of host aphid and host plant on mummy location and color plus improved methods for obtaining adults

    SciTech Connect

    Singh, R.; Hoy, M.A.

    2007-03-15

    , nosotros evaluamos el papel de otras especies de afidos y plantas hospederas potenciales sobre la produccion y ubicacion de las momias. Bajo condiciones de laboratorio, este parasitoide produjo el mayor numero de progenies sobre la especie de plaga enfocada, el afido pardo de los citricos sobre Citrus . Este parasitoide, no como la mayoria de los afidiidos (Hymenoptera), no produjo momias sobre cualquiera de las plantas hospederas probadas cuando fue criado sobre el afido negro de los citricos, ( T. aurantii (Boyer de Fonscolombe)) en toronja, el afido spirea (Aphis spiraecola Patch) en toronja y pittosporum, el afido del caupi (A. craccivora Koch) en toronja y caupi o el afido del melon (A. gossypii Glover) en toronja y pepino. Asi, el muestreo para momias de L. oregmae de estas especies de afidos hospederos y plantas hospederas tiene que incluir el mantenimiento de follaje en el laboratorio hasta que se produzcan las momias. Este parasitoide requiere una alta humedad relativa para producir los adultos por que ningun adulto emergio cuando las momias fueron mantenidas en capsulas de gelatina, pero una tasa alta de emergencia de adultos fue observada cuando se mantuvo las momias en platos con agar al 1.5%. Ademas de esto, nosotros comparamos el color de 6 especies de afidos hospederos, asi como el color de las momias producidos por L. oregmae cuando fue criado sobre ellos para determinar si se puede usar el color de las momias para la identificacion de L. oregmae . El color de las momias varia entre las especies de afidos hospederos y las plantas hospederas probadas, por lo que no es una herramienta util para la identificacion de L. oregmae o para la evaluacion de su efecto sobre los organismos que no son el enfoque del control. (author)

  7. Internet, Multimedia and Virtual Laboratories in a 'Third World' Environment.

    ERIC Educational Resources Information Center

    Monge-Najera, Julian Antonio; Rivas Rossi, Marta; Mendez-Estrada, Victor Hugo

    2001-01-01

    Describes the development of low-cost multimedia courses and materials for use on the Internet, as well as virtual laboratories, at the Universidad Estatal a Distancia (Costa Rica). Explains how simultaneous production of traditional printed materials and online courses, outsourcing, and the use of HTML and Java can reduce costs for developing…

  8. Revista Interamericana de Educacion de Adultos, 2003 (Interamerican Review of Adult Education, 2003).

    ERIC Educational Resources Information Center

    Guerra, Alfonso Rangel, Ed.

    2003-01-01

    This journal offers a collection of Spanish-language articles, including: "Educacion a Distancia y Uso de las Tecnologias: Experiencias Desafios y Oportunidades Educativas para Jovenes y Adultos" (Ma. Mercedes Ruiz); "Volver a a Educacion Fundamental? Notas para una Arquelogia de los Mandatos Fundacionales del CREFAL" (Jorge…

  9. ?`Y dónde está el turn-over?

    NASA Astrophysics Data System (ADS)

    Ostrov, P. G.

    La distribución de luminosidades de los cúmulos globulares presenta un máximo (turn-over) que no varía significativamente de galaxia en galaxia, por lo que es utilizada como indicador de distancia. En este trabajo se investigan los errores estadísticos de su determinación.

  10. On Categorizing Sounds.

    DTIC Science & Technology

    1987-11-17

    Introductino to its perceptual, neural, and social prospects. G. Stevens (Ed.), 1975, NY, Wiley. 11. Ward, L. M. & Lockhead, G. R. Category judgments...Objeto: Estructura y Procesos., S. Ballesteros and B. Shepp (Eds.), Universidad Nacional de Educacion a Distancia, Madrid, Spain, in press. 15

  11. Summary of Research 1997, Department of Operations Research.

    DTIC Science & Technology

    1999-01-01

    for addressing warfighting problems. This capabil- ity is especially important at the present time when technology in general, and information...Universidad Nacional de Educacion a Distancia, 1993 Master of Science in Operations Research-September 1997 Advisor: W. Max Woods, Department of

  12. Some Social Implications of Distance Education.

    ERIC Educational Resources Information Center

    Borden, George A.

    Social implications of distance education at the university level are considered, based primarily on the case of La Universidad Estatal a Distancia (UNED) of Costa Rica. UNED, which is Costa Rica's attempt to implement Great Britain's open university philosophy of education, was developed in response to the following needs: to provide…

  13. Analysis of the Use of Twitter as a Learning Strategy in Master's Studies

    ERIC Educational Resources Information Center

    Feliz, Tiberio; Ricoy, Carmen; Feliz, Sálvora

    2013-01-01

    The present study analyses the use of Twitter in the course Social Media and Digital Learning, taught as part of a master's programme at Universidad Nacional de Educacion a Distancia (Spain). The main purpose of the use of this resource was to develop a learning community. This was implemented through the micro design of activities. A mixed…

  14. Special Course on Shock-Wave/Boundary-Layer Interactions in Supersonic and Hypersonic Flows (Interactions entre Ondes de Choc et Couches Limites dans les Ecoulements Supersoniques et Hpersoniques).

    DTIC Science & Technology

    1993-08-01

    This leads to a helical separation vortex. One free-interaction analysis to demonstrate that (p,/p1 ), may view this vortex as a reorganization of...34 and the development of it underpredicted his data somewhat, while the a helical separation vortex. However, the lack of upstream-influence correlation...with a sinigle experliiieit".( Cobu-.litol it ii a ia laine. \\t a. 56. pp. 149-164. 60. D. VANDROMINE. lOS0. "Turi alence miodellliAi for varial ule

  15. Previniendo el Uso de Drogas entre Ninos y Adolescentes: Una Guia Basada en Investigaciones (Preventing Drug Use among Children and Adolescents: A Research-Based Guide).

    ERIC Educational Resources Information Center

    Sloboda, Zili; David, Susan L.

    This question and answer guide provides an overview of the research on the origins and pathways of drug abuse, the basic principles derived from effective drug abuse prevention research, and the application of research results to the prevention of drug use among young people. The basic principles derived from drug abuse prevention research are…

  16. Etudes sociales 30. Sujet B: Interaction entre nations. Cahier de l'eleve. Edition finale = Social Studies 30. Subject B: Interaction between Nations. Student Workbook. Final Edition.

    ERIC Educational Resources Information Center

    Alberta Dept. of Education, Edmonton. Curriculum Branch.

    In this Alberta (Canada) student workbook, four history units are presented for study. These are: (1) Between Two Wars (1919-1936); (2) World War II; (3) The Emergence and Interaction of the Superpowers; and (4) Contemporary World Interactions. Advice is offered to students in each unit along with information about key events and issues during…

  17. Thriving Together: Connecting Rural School Improvement and Community Development = Prosperando juntos: La conexion entre el mejoramiento de la escuela rural y el desarrollo comunitario.

    ERIC Educational Resources Information Center

    Boethel, Martha

    Available in English or Spanish, this resource guide aims to help rural schools and communities learn ways of supporting each other so that both can thrive. Background information and basic tools are provided for starting a joint school-community development effort. Chapters contain: (1) outline of the guide and statement of beliefs about…

  18. Vinculación Física entre Super Cáscaras de Hidrógeno Neutro y Formación Estelar Reciente

    NASA Astrophysics Data System (ADS)

    Corti, M. A.; Arnal, E. M.

    Many of the known galactic HI supershells (SCHI) may have been formed by the combined effects of stellar winds and supernovae explotions. Having most of them dynamic lifetimes of a few times 10^7 yr, these huge structures are likely to outlive the stars that gave rise to them. Hence, these SCHI may be used as fossil records of the Milky Way star formation history. Furthermore, along their evolution SCHI may trigger star formation. In this paper we analyze the genetic link put forward by McClure-Griffiths et al. (2002) between the OB association Cen OB1 and the SCHI GSH305+01-24. From our study we conclude that GSH305+01-24 may not represent a real physical structure. Instead, we believe that this structure is best represented by two unrelated huge HI shells. The role played by Cen OB1 in powering one of these HI shells is unclear for the time being. FULL TEXT IN SPANISH.

  19. Make the Reading-Writing Connection: Tips for Parents of Young Learners = Haga el enlace entre lectura y escrita: Consejos para padres de jovenes que estan aprendiendo.

    ERIC Educational Resources Information Center

    International Reading Association, Newark, DE.

    This brochure offers parents tips on helping their children connect writing with reading. It begins by noting that preschool children often want to scribble on paper, scribbles which sometimes represent a story. It suggests making space in the home for literacy, and encouraging early efforts. The brochure then offers four brief activities for…

  20. Entre dicho y hecho ...: An Assessment of the Application of Research from Second Language Acquisition and Related Fields to the Creation of Spanish CALL Materials for Lexical Acquisition

    ERIC Educational Resources Information Center

    Lafford, Barbara A.; Lafford, Peter A.; Sykes, Julie

    2007-01-01

    Despite the problems presented by lexical errors in second language (L2) communication, most computer assisted language learning (CALL) programs tend to focus on the acquisition of grammar points rather than on the development of the L2 lexicon. In addition, CALL vocabulary tasks are typically limited in scope and mechanical in nature, covering…

  1. Modelo Crosscultural de Pasantias para Lideres de la Educacion: Cooperacion entre Estados Unidos y Venezuela (Designing an Effective School Administrator Internship Program: United States and Venezuela Cooperation).

    ERIC Educational Resources Information Center

    Serafin, Ana Gil; Thompson, Eugene W.

    A model was developed of an internship program designed to give valuable cross-cultural experience to school administrators in training at universities in the United States and Venezuela. A naturalistic approach was used to develop the model. Leading school administrators and educational leadership theorists in both countries were interviewed by…

  2. La Linguistica Aplicada a la Relacion Paradigmatica entre los Verbos "Ser" y "Estar" (Linguistics Applied to the Paradigmatic Relationship between the Verbs "Ser" and "Estar")

    ERIC Educational Resources Information Center

    Marchetti, Magda Ruggeri

    1977-01-01

    Speakers of Italian often have problems mastering Spanish because they erroneously believe its great similiarity to Italian makes it easy to learn. One of the fundamental problems is the lack of ability to choose the correct verb, "ser" or "estar," both equivalents of the Italian "essere." (Text is in Spanish.) (CFM)

  3. NGC 5044-N50: Una etapa evolutiva intermedia entre galaxias compactas azules (BCD) y enanas elípticas (dE)

    NASA Astrophysics Data System (ADS)

    Cellone, S. A.; Buzzoni, A.

    The possible connection between dwarf elliptical (dE), dwarf irregular (dI), and blue compact dwarf (BCD) galaxies, is a subject of sustained interest, with deep consequences on our underestanding of star formation and evolution of galaxies. We present here observational results (ESO - CASLEO) on the dwarf galaxy N50 in the NGC 5044 Group, which appears to be at an intermediate stage BCD-dE, after its last star-formation burst. We focused on this galaxy because of its strong departure from the relation between luminosity and surface brightness profile curvature, despite its integrated color [(B-V)0=0.76], normal for a dE. However, new observations under sub-arcsec seeing conditions (ESO 3.6 m Tel.) showed several knots surrounding the nucleus whose colors (g-r, g-i, Gunn system) indicate, through evolutionary population synthesis models, ages around ~5 × 109 years with slightly sub-solar metallicities. The spectroscopic data support this scenario; hence, while the main component of N50 is an old (~ 15 × 109 yr) population, there is evidence of recent star formation activity within ~400 pc of the nucleus. The incidence of these structures on the anomalous profile of N50 is discussed, as well as the particular evolutive stage of this galaxy within the dE-BCD relation.

  4. Contribution à l'analyse des inter-relations entre activités humaines et variabilité climatique : cas du Sud forestier ivoirien

    NASA Astrophysics Data System (ADS)

    Yao, Télésphore Brou; Servat, Eric; Paturel, Jean-Emmanuel

    1998-12-01

    The South Ivorian Forest has suffered a large rainfall deficit for 25 years. At the same time, it has been noticed that the movement of the coffee and cocoa production seems concomitant with that of the isohyets during recent decades. The variations in albedo and rainfall gradient seem to be linked to the significant changes to the forest cover. These could affect precipitation locally.

  5. Relationship between Emotional Intelligence and Mental Health in School Counselors (Relación entre Inteligencia Emocional y salud mental en Orientadores Educativos)

    ERIC Educational Resources Information Center

    Cejudo, Javier

    2016-01-01

    Introduction: The purpose of the present research is aimed at studying the relationship between emotional intelligence as an ability and emotional intelligence as a trait and mental health of a sample of school counsellors. Method: The sample has been made up of 203 school counsellors. The instruments used have been: Mayer-Salovey-Caruso Emotional…

  6. Católicos, fidelidade conjugal e AIDS: entre a cruz da doutrina moral e as espadas do cotidiano sexual dos adeptos1

    PubMed Central

    Rios, Luis Felipe; de Aquino, Francisca Luciana; Muñoz-Laboy, Miguel; Oliveira, Cinthia; Parker, Richard

    2009-01-01

    Neste artigo discutimos a visão da Igreja Católica sobre sexualidade na interface com a epidemia do HIV/AIDS. Nossa reflexão está embasada em pesquisa etnográfica que envolveu dois meses de observação participante do cotidiano de católicos de um bairro popular da Região Metropolitana do Recife, além de contar com entrevistas a onze dos leigos engajados nos serviços religiosos da igreja do bairro e a oito sacerdotes que realizam seus trabalhos religiosos em outras localidades. Nelas abordamos diferentes temáticas relacionadas ao enfrentamento da epidemia da AIDS. Nesse contexto, conjugalidade e fidelidade se afiguram como importantes analisadores de como aqueles lidam com a epidemia, em uma variedade de re-descrições práticas e de re-interpretações conceptuais das assertivas do discurso moral religioso – ainda que, muitos impasses permaneçam em aberto em termos das prerrogativas da Igreja e seus possíveis rebatimentos na saúde sexual dos adeptos. PMID:21765650

  7. Entre dois mundos: vida quotidiana de criancas portuguesas na america [Between Two Worlds: The Daily Life of Portuguese Children in America].

    ERIC Educational Resources Information Center

    de Meneses, Fernando

    This Portuguese reader with accompanying teacher's guide describes the culture shock that Portuguese children and their families face when they immigrate to North America. The reader is divided into 75 short stories about the families that come from the rural areas of Portugal where there has been little industrialization. The purpose of the…

  8. Uma comparação entre técnicas de propagação de erros em astrofísica: Monte Carlo x Bootstrap

    NASA Astrophysics Data System (ADS)

    Waelkens, A. H.; Janot Pacheco, E.

    2003-08-01

    Recentemente Dhillon & Watson (2001) criticaram o procedimento de determinação de erros via simulações de Monte Carlo no caso do imageamento indireto de estrelas, por introduzir ruído nos dados e resultar em erros superestimados. Estes autores propuseram a utilização alternativa do método de Bootstrap como uma técnica superior para a estimativa de erros. Neste trabalho apresentamos os resultados de um estudo comparativo da performance das técnicas de simulação por Monte Carlo e por Bootstrap para a determinação de erros em duas situações de interesse: (i) ajuste de modelos a espectros observados e (ii) imageamento indireto de discos de acréscimo com técnicas de mapeamento por eclipse. No caso (i), os dados de entrada são espectros de emissão simples de hidrogênio aos quais é adicionado ruído gaussiano de amplitude selecionada. No caso (ii), são produzidas curvas de luz do eclipse de uma distribuição de brilho seguindo a lei TµR-3/4 para discos estacionários cuja taxa de acréscimo varia ao longo do eclipse segundo uma distribuição gaussiana de amplitude selecionada, simulando o ruído. A avaliação da performance é feita com o auxílio de um diagrama do erro relativo na grandeza ajustada, conforme obtido por cada método, versus o erro relativo (ruído/sinal) dos dados de entrada. O estudo mostra que as duas técnicas produzem resultados semelhantes nos dois casos, e que a suposta superioridade do método de Bootstrap não se confirma.

  9. Étude de la transition entre le gaz atomique et le gaz moléculaire dans deux cirrus de la boucle céleste nord

    NASA Astrophysics Data System (ADS)

    Barriault, Léo

    2010-11-01

    The infrared (IR) cirrus clouds observed at high Galactic latitude are excellent candidates to study the transition between the atomic gas and the molecular gas. In this thesis, two potential sites of H2 formation have been discovered in the North Celestial Loop (l = 135°, b = 40°) through examining the far-IR-Hl ratio and looking for an excess over that expected from an atomic medium. I call these sites the Spider and Ursa Major fields. The comparison between the IR excess map and the 12CO (J - 1 - 0) data from the Five College Radio Astronomical Observatory (resolution = 45 arcsec) shows that the IR excess peaks do not coincide with the 12CO peaks. The absence of coincidence is explained by (i) a density too small to allow CO excitation, (ii) insufficient CO self-shielding or (iii) variations of the dust properties. The comparison between 12CO data and Hi data from the Dominion Radio Astrophysical Observatory (resolution = 1 arcmin) is in agreement with the models! that predict CO formation where large velocity shears and turbulence dissipation are observed. OH observations of 108 locations over the two diffuse clouds from the Green Bank Telescope (resolution = 7 arcmin) are analyzed. OH is a precursor molecule to CO and its formation requires H2. The coincidence between the OH emission peak and the IR excess peak indicates that OH could be a better tracer of H2 than CO in these low-density regions. 12CO (7 = 1-0) and 13CO (J = 1 -0) observations from the Institut de Radioastronomie Millimétrique and 12CO ( J = 2 - 1) observations from the James Clerk Maxwell Telescope (resolution = 20 arcsec) are analyzed on a small number of fields in both regions. Using a large velocity gradient model, we found smaller densities at the location of the IR excess peak while self-shielding should be efficient given the computed column densities.

  10. L'Harmonisation des langues Maliennes: Entre l'intregration nationale et regionale (The Harmonization of the Malian Languages: Between National and Regional Integration).

    ERIC Educational Resources Information Center

    Ouane, Adama

    1991-01-01

    Assesses efforts to standardize the Malian languages, including the standardization of orthography and the creation of terminology in the Mandingo-Peul Project. Points to conflicts between technical and political considerations, and between local tendencies and balances among languages and the need to respect the ecolinguistics of the country.…

  11. Entre le mythe et l'histoire: L'oscillation du romanesque (Between Myth and History: The Oscillation of the Romanticism).

    ERIC Educational Resources Information Center

    Tremblay, Victor Laurent

    1989-01-01

    A discussion of Romantic literature examines the links between literature and history and a mythic mechanism, a recurrent polarization between two opposite trends (myth and history) at the level of discourse, symbols, and representation. Mytho-romantic and neo-romantic are distinguished and examined. (Author/MSE)

  12. Entre didactique et pedagogie: Epistemologie de l'espace/temps strategique (Between Instruction and Pedagogie: The Epistemology of Strategic Time/Space).

    ERIC Educational Resources Information Center

    Tochon, Francois, V.

    1991-01-01

    Analyzes the components of teaching, envisioning a strategic moment when instruction and pedagogy merge. Examines the way in which transformations of knowledge could be represented according to recent trends in artificial intelligence. (DMM)

  13. EXPERIENCIAS RELACIONADAS A UNA INTERVENCIÓN PARA REDUCIR EL ESTIGMA RELACIONADO AL VIH/SIDA ENTRE ESTUDIANTES DE MEDICINA EN PUERTO RICO

    PubMed Central

    Cintrón-Bou, Francheska; Varas-Díaz, Nelson; Marzán-Rodríguez, Melissa; Neilands, Torsten B.

    2016-01-01

    Existe estigma relacionado al VIH. A las personas con VIH/SIDA-PCVS se les viola sus derechos y obstaculiza su bienestar mental/físico. Profesionales de la salud-PS son fuente de apoyo primordial, sin embargo estos/as le estigmatizan. Es útil adiestrar a PS en relación al estigma social. Implantamos la intervención para reducir el estigma relacionado al VIH/SIDA con 507 estudiantes de medicina. Resultó ser una intervención efectiva, hubo reducción en los niveles de estigma a partir de nuestra intervención y diferencias significativas con el grupo control (p≤.05). Generar espacios de adiestramiento para atender el estigma relacionado al VIH/SIDA es pertinente para la psicología comunitaria porque colaboramos en la reducción de actitudes estigmatizantes que afectan adversamente la prevención de nuevas infecciones, la adherencia al tratamiento antirretroviral y la calidad de vida. PMID:27829690

  14. L'espace du mouvement: Une analyse des conflits dans les interactions entre institutrice et eleves d'une ecole maternelle

    NASA Astrophysics Data System (ADS)

    Galvão, Izabel

    1995-01-01

    The research described in this article, winner of the Gottfried Hausmann Prize for 1994, was carried out in a kindergarten in São Paolo, Brazil, using the theory developed by Henri Wallon. The aim was to examine how primary school children are affected in their development by: (a) unnecessary restrictions placed on their physical movements during lessons; and (b) failure to organize the classroom space in a way that assists the learning process. The author concludes that these factors lead to tensions in the classroom which could be avoided through a different approach to movement and space.

  15. Relación física entre el cúmulo abierto Hogg 15 y la estrella Wolf-Rayet WR 47

    NASA Astrophysics Data System (ADS)

    Piatti, A. E.; Bica, E.; Santos, J. F. C., Jr.; Clariá, J. J.

    We revise the fundamental parameters of the faint open cluster Hogg 15, for which two recent colour-magnitude diagram (CMD) studies have obtained significantly different ages (Sagar et al. 2001, MNRAS, 327, 23; Piatti & Clariá 2001, A&A, 370, 931). In the present study, we combine a series of methods trying to constrain age, together with other fundamental parameters. We employ spatial extractions to construct the CMDs, and the cluster integrated spectrum to compare the latter with those of templates of known age. For Hogg 15 we derive an age of (20 ± 10) Myr, a reddening of (1.10 ± 0.05) mag, and a distance of (3.1 ± 0.5) kpc. We conclude that the estimation of an older age in the study of Piatti & Clariá can be accounted for in terms of main sequence/turnoff curvature being blurred in the CMDs, an effect mainly caused by field contamination. To clarify the issue of whether the Wolf-Rayet star HDE 311884 (WR 47), known to be a WN6 + O5V binary system, is associated or not with Hogg 15, we discuss its fundamental parameters, particularly its distance. Based on the WR 47 spectrum and available photometry, we assume that the underestimated distance implied by the Hipparcos parallax (216 pc) is affected by its binary character. By comparing the WR 47 spectrum with those of WR stars of a similar type, we conclude that WR 47 is not affected by a E(B-V) colour excess much higher than that associated with Hogg 15, namely E(B-V) = 1.10. On the basis of the WR 47's resulting distance of (5.2 ± 0.9) kpc, which largely surpasses that of the cluster, we conclude that WR 47 is not related to Hogg 15 from the point of view of origin, since the cluster and the star do not belong to the same formation event.

  16. Étude de l'interdiffusion entre l'argent et le palladium dans des couches minces par spectrométrie auger

    NASA Astrophysics Data System (ADS)

    Guglielmacci, J. M.; Gillet, M.

    1981-04-01

    We investigate the interdiffusion in epitaxial thin films of Ag(111)-Pd(111) heated at 500°C during 15 min. The interdiffusion coefficient D is determined by two methods: (1) the concentration profile is established using the Auger technique associated with sputtering; (2) we follow the surface concentration during the diffusion process. This last technique avoids problems associated with ion sputtering. The two techniques give interdiffusion coefficients D in good agreement with a mean value of 7 × 10 -17 cm 2 s -1.

  17. L'accident du Toulourenc : une limite tectonique entre la plate-forme provençale et le Bassin vocontien à l'Aptien?Albien (SE France)

    NASA Astrophysics Data System (ADS)

    Montenat, Christian; Janin, Marie-Christine; Barrier, Pascal

    2004-11-01

    The WNW-ESE trending Toulourenc Fault Zone (TFZ) is the western segment of the major Ventoux-Lure Fault Zone, which separates the Provençal platform from the Baronnies Vocontian Basin. The TFZ was subject to polyphased Mid-Cretaceous movements, during the Early Aptian and Middle-Late Albian times. The latter faulting episode generated conglomerates and olistoliths resulting from dismantled faultscarps cutting Barremian-Bedoulian limestones. The deformation is related to compressional wrench faulting (NE-SW sinistral faults; dextral component for the TFZ). It induced the uplift of the northwestern corner of the platform, as indicated by a mid-Cretaceous hiatus (Early Aptian pro parte to Early Albian) narrowly delimited in space. The opening of submeridian grabens within the platform favoured the northward transit of channelised coarse-grained Albian sands originating from a southern area. To cite this article: C. Montenat et al., C. R. Geoscience 336 (2004).

  18. En la búsqueda de características en eyecciones coronales de masa que discriminen entre dos paradigmas físicos en modelos de ECMs

    NASA Astrophysics Data System (ADS)

    Paissan, G.; Stenborg, G.; Rovira, M.

    Se conocen tres diferentes fenómenos de gran escala que ocurren en la atmósfera solar, denominados eyecciones coronales de masa (ECMs), protuberancias eruptivas y grandes fulguraciones de dos bandas. Estos fenómenos están estrechamente relacionados y podrían ser distintas manifestaciones de un único proceso físico. Las ECMs son definidas como eyecciones de gran escala de masa y flujo magnético desde la baja corona al espacio interplanetario. Desde su descubrimiento en los '70, muchos modelos han sido propuestos para explicar su origen y evolución. La explicación física de las ECMs es un tema de debate intenso. No obstante, los modelos pueden sintetizarse en dos grandes grupos: 1) los modelos de inyección de flujo y 2) los modelos de almacenamiento y liberación. En este trabajo, se presentan los estudios realizados con una serie de eventos observados con el coronógrafo MICA (Mirror Coronograph for Argentina), el telescopio en H-alfa HASTA (H-alpha Solar Telescope for Argentina) y los coronógrafos C2 y C3 de la sonda SOHO (Solar and Heliospheric Observatory). Los eventos que pudieron ser identificados como ECMs son contrastados dentro del esquema de los dos paradigmas teóricos propuestos.

  19. Champ dynamique dans une cellule d'échangeur à vortex : 1. Régime d'entrée laminaire

    NASA Astrophysics Data System (ADS)

    Petitot, Stéphane; Lyazid, Abdel; Devienne, René

    Numerical simulation and LDV measurements are performed for laminar inlet flow condition in a vortex exchange chamber with isothermal conditions. Newtonian and non-Newtonian fluids were used. Experimental measurements are compared to results of numerical computations with good agreement. Both show the existence of a secondary flow generated by hydrodynamical instabilities due to streamline curvature. The main vortex flow appears then to be restricted by this secondary flow.

  20. L'Alternance entre les auxiliaires "avoir" et "etre" en francais parle a Montreal (Alternation between the Auxiliaries "Avoir" and "Etre" in Spoken French in Montreal)

    ERIC Educational Resources Information Center

    Sankoff, Gillian; Thibault, Pierrette

    1977-01-01

    A study of the spoken French of 120 residents of Montreal. The researchers were interested in the linguistic and social factors influencing the use of "avoir" and "etre." Results show that this usage corresponds to a tendancy to regularize conjugations. (Text is in French.) (AMH)

  1. Relación entre la orientación de Nebulosas Planetarias y el campo magnético galáctico

    NASA Astrophysics Data System (ADS)

    Weidmann, W.; Díaz, R. J.; Carranza, G.

    Un estudio detallado de las orientaciones diferenciales de nebulosas planetarias en todo el cielo sugiere fuertemente una relación con la dirección de los campos magnéticos de origen galáctico, determinados en la polarización de estrellas de campo. Esto da consistencia a la hipótesis de que la orientación de las nebulosas planetarias ovaladas es inducida, al menos parcialmente, por el campo magnético general de la Vía Láctea.

  2. Evidence supporting a promotora-delivered entertainment education intervention for improving mothers' dietary intake: the Entre Familia: Reflejos de Salud Study.

    PubMed

    Ayala, Guadalupe X; Ibarra, Leticia; Horton, Lucy; Arredondo, Elva M; Slymen, Donald J; Engelberg, Moshe; Rock, Cheryl L; Hernandez, Erika; Parada, Humberto; Elder, John P

    2015-01-01

    Entertainment education and the promotora model are 2 evidence-based health communication strategies. This study examined their combined effect on promoting healthy eating among mothers in a family-based intervention. Participants were 361 Mexican-origin families living in Imperial County, California, who were randomly assigned to an intervention or delayed treatment condition. The intervention involved promotoras (community health workers) who delivered 11 home visits and 4 telephone calls. Home visits included a 12-minute episode of a 9-part situation comedy depicting a family struggling with making healthy eating choices; an accompanying family workbook was reviewed to build skills and left with the family. Baseline and immediate postintervention data were collected from the mothers, including the primary outcome of daily servings of fruits and vegetables. Other dietary and psychosocial factors related to healthy eating were examined. At postintervention, mothers in the intervention reported increases in daily vegetable servings (p ≤ .05); however, no changes were observed in fruit consumption. Improvements were observed in behavioral strategies to increase fiber (p ≤ .001) and to decrease fat intake (p ≤ .001), unhealthy eating behaviors (p ≤ .001), and individual (p ≤ .05) and family-related (p ≤ .01) perceived barriers to healthy eating. Entertainment education and promotoras engaged families and improved mothers' diets. Further research should examine the dose needed for greater changes.

  3. Optimisation de l'émission du continuum femtoseconde de lumière blanche entre 600 nm et 800 nm

    NASA Astrophysics Data System (ADS)

    Ramstein, S.; Mottin, S.

    2005-06-01

    Un dispositif de spectroscopie avec résolution du temps de vol des photons en milieu diffus a été développé. Celui-ci repose sur l'utilisation d'un continuum de lumière blanche généré par focalisation d'un laser amplifié (830 nm, 1 kHz, 0.5 W, 170 fs) dans de l'eau déminéralisée. Afin d'optimiser spectralement et en puissance la source blanche sur la fenêtre spectrale 600 800 nm, une étude de la mise en forme spatio-temporelle avant autofocalisation de l'impulsion laser par le milieu a été menée. Cette mise en forme est effectuée de manière spatiale en changeant la focale de la lentille de focalisation et de manière temporelle en changeant le taux de compression de l'impulsion. L'étude montre que le cône de lumière émise possède plus de puissance dans la fenêtre spectrale d'intérêt pour des focales longues. Sur la fenêtre 600-800 nm, le rendement énergétique intégré varie de 5%, avec une focalef=6cm, à 15%, avec une focale f = 30 cm. La mise en forme temporelle montre des effets similaires avec les mêmes ordres de grandeur.

  4. La Littérature De Jeunesse Entre Normes Pédagogiques Et Littéraires : Le Cas Des Pays Francophones D'afrique

    NASA Astrophysics Data System (ADS)

    Attikpoé, Kodjo

    2007-01-01

    YOUTH LITERATURE IN THE CONTEXT OF PEDAGOGIC AND LITERARY NORMS: THE FRENCH-SPEAKING COUNTRIES OF AFRICA - There are two approaches to children's and youth literature, one didactic, the other aesthetic. This gives rise to the following question: taking into account the fact that children's literature aspires to be a literary form in its own right, should more weight be attibuted to its aesthetic value when evaluating it? This paper examines this question - which has in the past been the subject of highly controversial discussions in the German-speaking regions - by focusing on youth literature in the French-speaking coutries of Africa. The pedagogic dimension cannot be ignored when considering this literary context because it is so much a part of the post-colonial dynamic. Youth literature in the French-speaking countries of Africa echoes current realities. Examples are used to demonstrate how literature can deal with vital questions without neglecting the literary aspect.

  5. Psychological Correlates of the Transmission and Acceptance of Rumors about AIDS = Correlates Psychologiques Entre la Transmission et l'Acceptation des Rumeurs sur le SIDA.

    ERIC Educational Resources Information Center

    Kimmel, Allan J.

    Public reactions to the Acquired Immune Deficiency Syndrome (AIDS) crisis are accompanied by the sorts of emotional and cognitive factors that have been identified by researchers as predictors of rumor generation and transmission. The present investigation attempted to ascertain the extent that various subjective factors (including anxiety,…

  6. Food intake and nutritional status influence outcomes in hospitalized hematology-oncology patients.

    PubMed

    Calleja Fernández, Alicia; Pintor de la Maza, Begoña; Vidal Casariego, Alfonso; Villar Taibo, Rocío; López Gómez, Juan José; Cano Rodríguez, Isidoro; Ballesteros Pomar, María D

    2015-06-01

    Introducción: la malnutrición en el paciente oncohematológico es importante debido a su prevalencia y a su morbimortalidad asociadas. El objetivo de este estudio fue analizar la prevalencia de malnutrición en el paciente oncohematológico y determinar si la ingesta o la malnutrición afectan a las complicaciones del paciente hospitalizado. Metodología: estudio de corte realizado en todos los pacientes admitidos en las plantas de oncología y hematología durante un periodo de 30 días. La valoración nutricional se realizó durante las 24 primeras horas tras el ingreso y se repitió a los 7 días de hospitalización, incluyendo Valoración Subjetiva Global, antropometría, recuerdo de 24 horas y estimación de las necesidades calóricas y proteicas. Las historias médicas fueron revisadas a los 30 días tras el alta. Resultados: setenta y tres pacientes fueron evaluados al ingreso y 29 a los siete días de su hospitalización. La prevalencia de malnutrición fue 47,7%. Al ingreso, los pacientes consumieron 71,6 (DE 22,0)% de las calorías prescritas y 68,2 (DE 22,0)% de las proteínas prescritas. La tasa de fallecimientos fue 2,8% entre los pacientes que consumieron ≥75% y 17,9% entre aquellos que consumieron.

  7. Herb-drug Interactions: An insight into cardiovascular diseases based on case reports.

    PubMed

    Campos, Maria; Batista, C; Jesus, N R; Silva, C M; Silva, T P

    2016-10-07

    Cardiovascular patients frequently use herbal medicinal products, in order to contribute to the improvement of their chronic condition without medical intervention. However, they are likely to suffer from adverse effects from natural products and herb-drug interactions. This work aimed to alert cardiovascular patients and healthcare providers of the potential of occurrence of herb-drug interactions with cardiovascular therapy. Information obtained from the campaign "Aprender Saúde Entre as Plantas e os Medicamentos", carried out by the Observatory of Herb-Drug Interactions (www.oipm.uc.pt), was accessed in order to exemplify some selected interactions. From data received during the campaign, it was highlighted the prevalence of certain natural products particularly goji berries, green tea, mangosteen and rooibos that have significant cardiovascular effects. For this reason their intake should be carefully monitored in cardiovascular patients. This prevalence of use suggests a pattern in their use in Portugal. The ending results also indicate that there is still a lack of knowledge about the possible risks of herbal products, which may adversely affect the health of any patient. Thus becomes clear the value of the role of health professionals in the screening of such interactions.

  8. Trait differences between naturalized and invasive plant species independent of residence time and phylogeny

    PubMed Central

    Gallagher, R V; Randall, R P; Leishman, M R

    2015-01-01

    inclusion of quantitative traits, in particular SLA, into the WRA schemes. Diferencia de Características entre Especies de Plantas Naturalizadas e Invasoras Independientes del Tiempo de Residencia y de la Filogenia Resumen La habilidad para predecir cuáles plantas exóticas harán la transición de naturalizadas a invasoras antes de su introducción a regiones nuevas es un objetivo clave para la conservación y tiene el potencial de incrementar la eficiencia de la evaluación de riesgo de hierbas (ERH). Sin embargo, múltiples factores contribuyen al éxito invasor de las plantas (p. ej.: características funcionales, características de cobertura, tiempo de residencia, filogenia) y todos deben considerarse simultáneamente para poder identificar correlaciones significativas del éxito invasor. Recopilamos en Australia 146 parejas de especies de plantas invasoras y naturalizadas emparejadas filogenéticamente (congéneres) y con tiempos de residencia mínima similares (es decir, el tiempo transcurrido desde su introducción en años). Estas parejas se usaron para probar diferencias en cinco características funcionales (duración de la floración, tamaño de la hoja, altura máxima, área específica de la hoja [AEH], masa de la semilla) y en tres características de cobertura nativa de las especies (ocupación de bioma, temperatura media anual y amplitud de pluviosidad) entre especies invasoras y naturalizadas. Las especies invasoras, en promedio, tuvieron una mayor AEH, periodos de floración más largos y fueron más altas que sus parientes congéneres naturalizadas. Las invasoras también exhibieron una mayor tolerancia a diferentes condiciones ambientales en su cobertura nativa, donde ocuparon más biomas y una mayor amplitud de pluviosidad y condiciones de temperatura que sus congéneres naturalizadas. Sin embargo, ni la masa de la semilla ni el tamaño de hoja difirieron entre las parejas de especies naturalizadas e invasoras. Un hallazgo relevante fue el papel de

  9. [FACTORS AFFECTING THE ABANDONMENT OF BREASTFEEDING IN A SUPPORT PROGRAM FOR THE SAME IN THE HOSPITAL FOR WOMEN, MORELIA MICHOACAN IN THE PERIOD FROM SEPTEMBER TO NOVEMBER 2014].

    PubMed

    Méndez Jacobo, Névedy; García Rojas Vazquez, Luisa Estefanía; Reyes Barretero, Diana Yolanda; Trujano Ramos, Luis Alfredo

    2015-12-01

    Introducción: existen ciertos factores que influyen en el abandono precoz de la lactancia materna, entre los cuales se encuentran los sociodemográficos, culturales, biológicos y psicológicos. Objetivo: analizar los factores sociodemográficos que influyen en el abandono de la lactancia materna en un grupo de apoyo a la misma. Metodología: estudio descriptivo, prospectivo, observacional y transversal, en el Hospital de La Mujer en Morelia, Michoacán, de septiembre a noviembre del 2014, con un total de 86 mujeres en periodo de puerperio de la planta de ginecología. Resultados: las causas por las cuales se abandona la LME son: edad menor de 25 años (19.1%), localidad urbana (16.9%), estado civil unión libre (15.7%) y por el trabajo (9%). Conclusiones: en la mayor parte de las mujeres la edad, el nivel educativo y la localidad son los principales factores que influyen en cuanto al abandono de la lactancia materna antes del tiempo recomendado.

  10. When Did the Swahili Become Maritime?

    PubMed Central

    Fleisher, Jeffrey; Lane, Paul; LaViolette, Adria; Horton, Mark; Pollard, Edward; Quintana Morales, Eréndira; Vernet, Thomas; Christie, Annalisa; Wynne-Jones, Stephanie

    2015-01-01

    esta sociedad costera entre el primer y segundo milenio. Éstas son la variabilidad y discontinuidad en la localización y permanencia de los asentamientos; evidencia de una conexión mayor con el mar a través de la tecnología de pesca y navegación; y desarrollos arquitectónicos especializados que incluyen instalaciones portuarias, mezquitas, y casas. Las implicaciones de este estudio indican que debemos considerar otros aspectos de una sociedad aparte de su localización costera para determinar su maritimidad. Hay que considerar cómo el mar y sus productos son parte de la vida social y evaluar si existe una influencia recíproca entre el ambiente marítimo y los patrones de organización sociocultural, las prácticas, y las creencias de los Swahili y otras sociedades. [marítimo, pesca y navegación, comercio a larga distancia, Swahili, África Oriental] PMID:25821235

  11. [CONCORDANCE IN THE RESULTS OF CONTROL INTAKE PERFORMANCE OF 72 H BY DIFFERENT HEALTH PROFESSIONALS IN A TERTIARY HOSPITAL].

    PubMed

    Benítez Brito, Néstor; Mora Mendoza, Alejandra; Suárez Llanos, José Pablo; Delgado Brito, Irina; Pérez Méndez, Lina I; Herrera Rodríguez, Eva María; Oliva García, José Gregorio; Pereyra-García Castro, Francisca

    2015-12-01

    Introducción: el cribado nutricional CIPA es positivo cuando se cumple uno de los siguientes parametros: Control de Ingestas (CI) 72 h < 50%; albumina < 3 g/dl; Indice de Masa Corporal (IMC) < 18,5 kg/m2 o Circunferencia del Brazo (CB) ≤ 22,5 cm (si no se puede determinar IMC). El unico parametro no totalmente objetivo es el CI, por lo que este estudio busca reforzar la validez del mismo. Objetivos: analizar la concordancia existente entre diferentes profesionales sanitarios a la hora de evaluar un CI de 72 h. Material y métodos: estudio prospectivo de pacientes ingresados en plantas de hospitalizacion (febrero-octubre de 2014). Variables recogidas: edad, sexo, servicio medico, CI de 72 h y tipo de dieta. El objetivo del CI es valorar la cantidad de alimento consumido, diferenciando entre ingestas superiores o inferiores al 50%. Se analiza la concordancia de los resultados del CI dictaminados por un medico, una enfermera y un nutricionista (gold-standar) mediante el coeficiente Kappa (K). Posteriormente se calculo la sensibilidad (S) y la especificidad (E) de los CI positivos. Resultados: se analizaron 176 controles de ingestas. Edad: 63,70 } 14,46 anos; 42% mujeres. Servicios: Digestivo (22,2%), Neumologia (42%), Medicina Interna (21,6%), Nefrologia (9,1%), Otros (5,1%). Tipo de dieta: basal (23,3%), terapeutica (67%), consistencia (4%), progresion (3,4%), otros (2,3%). Se comparo el CI de enfermeria vs. nutricionista (K = 0,798; p < 0,001), y del medico vs. nutricionista (K = 0,823; p < 0,001). La S y E de enfermeria vs. nutricionista fue de 84% y 97%, mientras que entre medico y nutricionista fue de 84% y 98%, respectivamente. Conclusiones: la concordancia de los resultados de un CI de 72 horas (ingestas < o > al 50%) realizado en pacientes hospitalizados por un medico, una enfermera y un nutricionista es alta, restando subjetividad a este parametro. El CI resulta util para su uso dentro de la practica clinica habitual y, en concreto, dentro de cribados

  12. The Spanish National Reference Database for Ionizing Radiations (BANDRRI)

    PubMed

    Los Arcos JM; Bailador; Gonzalez; Gonzalez; Gorostiza; Ortiz; Sanchez; Shaw; Williart

    2000-03-01

    The Spanish National Reference Database for Ionizing Radiations (BANDRRI) is being implemented by a reasearch team in the frame of a joint project between CIEMAT (Unidad de Metrologia de Radiaciones Ionizantes and Direccion de Informatica) and the Universidad Nacional de Educacion a Distancia (UNED, Departamento de Mecanica y Departamento de Fisica de Materiales). This paper presents the main objectives of BANDRRI, its dynamic and relational data base structure, interactive Web accessibility and its main radionuclide-related contents at this moment.

  13. Farmyard Manure and Fertilizer Effects on Seed Potato (Solanum tuberosum L.) Yield in Green House Production

    NASA Astrophysics Data System (ADS)

    László, M.

    2009-04-01

    alta potencialidade (boa textura e adequada retenção de agua). Efeito do nitrogênio No Brazíl em todas regiões onde se cultiva batata é raro encontrar solos com elevado teor de nitrogênio e que não precisam de quantidades desta elemento. É o elemento que governa o padrão de desenvolvimento da planta, estimulando principalmente o crescimento da parte aérea (Kádár et all. 2000., László 2000.). Para se obter rendimento de tubérculos é necessário um rápido e curto periodo de desenvolvimento da parte aérea e uma fase de acúmulo maior possível. Para tanto, devem ser evitadas doses muito altas de nitrogênio e, principalmente, se aplicadas tardiamente, pois haverá demasiado desenvolvimento de folhas que demorarão mais a finalizar seu crescimento e maturação. Isto implicará na redução do periodo de intenso desenvolvimento dos tubérculos e armazenagem do amido, resultando em menor produção. Há risco para a saúde na ingestão de alimentos com altos teores de nitrato e nitrito, havendo preocupação com o efeito de doses mais altas de nitrogênio sobre os teores dos mesmos nos tuberculos. Embora haja diferenças entre cultivares, localidades e períodos de armazenamento, há estudos mostrando que a fertilização com até 150 kg ha-1 de nitrogênio não foi suficiente para propiciar niveis preocupantes de nitrato nos tuberculos colhidos. Efeito do fósforo A grande maioria dos solos brasileiros cultivados com batata apresentam limitações ao bom desenvolvimento e produção da cultura em função dos baixos níveis de fósforo. Isto significa que aumentos de produção quase sempre ocorrem quando o solo recebe adubação fosfatada (László 2001a.). Estes aumentos são mais marcantes em solos nunca antes adubados ou com baixo teor de fósforo. Nestes, dificilmente alcanca- se a produção máxima com níveis reduzidos de fósforo. Não seria exagerado supor níveis de 600 a 800 kg ha-1 de P2O5, como sendo aqueles que iriam proporcionar produ

  14. CONTAMINACIÓN AMBIENTAL, VARIABILIDAD CLIMÁTICA Y CAMBIO CLIMÁTICO: UNA REVISIÓN DEL IMPACTO EN LA SALUD DE LA POBLACIÓN PERUANA

    PubMed Central

    Gonzales, Gustavo F.; Zevallos, Alisson; Gonzales-Castañeda, Cynthia; Nuñez, Denisse; Gastañaga, Carmen; Cabezas, César; Naeher, Luke; Levy, Karen; Steenland, Kyle

    2015-01-01

    RESUMEN El presente artículo es una revisión sobre la contaminación del agua, el aire y el efecto del cambio climático en la salud de la población peruana. Uno de los principales contaminantes del aire es el material particulado menor de 2,5 μ (PM 2,5), en la ciudad de Lima, anualmente 2300 muertes prematuras son atribuibles a este contaminante. Otro problema es la contaminación del aire domiciliario por el uso de cocinas con combustible de biomasa, donde la exposición excesiva a PM 2,5 dentro de las casas es responsable de aproximadamente 3000 muertes prematuras anuales entre adultos, con otro número desconocido de muertes entre niños debido a infecciones respiratorias. La contaminación del agua tiene como principales causas los desagües vertidos directamente a los ríos, minerales (arsénico) de varias fuentes, y fallas de las plantas de tratamiento. En el Perú, el cambio climático puede impactar en la frecuencia y severidad del fenómeno de El Niño oscilación del sur (ENSO) que se ha asociado con un incremento en los casos de enfermedades como cólera, malaria y dengue. El cambio climático incrementa la temperatura y puede extender las áreas afectadas por enfermedades transmitidas por vectores, además de tener efecto en la disponibilidad del agua y en la contaminación del aire. En conclusión, el Perú, pasa por una transición de factores de riesgo ambientales, donde coexisten riesgos tradicionales y modernos, y persisten los problemas infecciosos y crónicos, algunos de los cuales se asocian con problemas de contaminación de agua y de aire. PMID:25418656

  15. Positive Prevention: Successful Approaches To Preventing Youthful Drug and Alcohol Use [and] La Prevencion Positiva: Metodos que han tenido exito en la prevencion del uso de drogas y alcohol entre la juventud.

    ERIC Educational Resources Information Center

    American Association of School Administrators, Arlington, VA.

    The United States has the highest rate of youthful drug abuse of any industrialized country in the world. There is a growing awareness that drug and alcohol use are closely connected to other problems such as teenage suicide, adolescent pregnancy, traffic fatalities, juvenile delinquency, poor school performance, runaways, and dropouts. Youthful…

  16. Online Peer Feedback between Colombian and New Zealand FL Beginners: A Comparison and Lessons Learned (Retroalimentación virtual de pares entre aprendices principiantes de lengua de Colombia y Nueva Zelanda: una comparación y lecciones aprendidas)

    ERIC Educational Resources Information Center

    Tolosa, Constanza; Ordóñez, Claudia Lucía; Alfonso, Tania

    2015-01-01

    We report on an exploratory study comparing the performance as online tutors of two groups of beginner eleven-year-old students of English in Colombia and Spanish in New Zealand. The native speaker students of the foreign language the others were learning corrected paragraphs written by their peers. The feedback provided by each group of tutors…

  17. Exploring Elementary Students' Power and Solidarity Relations in an EFL Classroom (Exploración de las relaciones de poder y solidaridad entre estudiantes de primaria en la clase de inglés como lengua extranjera)

    ERIC Educational Resources Information Center

    Méndez, Tatiana; García, Andrea

    2012-01-01

    This article derives from a critical discourse analysis study that reports the characteristics of elementary school students' power and solidarity relations in English as a foreign language classroom in Bogotá, Colombia, while we were doing our teaching English as a foreign language practicum. The study was based on theories of power and…

  18. La Desercion Escolar en los Niveles de Sexto y Noveno Grado: Una Comparacion Entre Zonas Rurales y Urbanas. Boletin 219 (The Educational Desertion in the 6th and 9th Grades: A Comparison Between Rural and Urban Zones. Bulletin 219).

    ERIC Educational Resources Information Center

    Lopez, Maria I. Hernandez; And Others

    In 1962 and again in 1965, a group of sixth and ninth grade students in selected schools in four Puerto Rican communities were interviewed to investigate the factors influencing students' decision to withdraw from school before completing twelfth grade and to identify some characteristics of dropouts. Of 616 students interviewed in 1962, 20.9% of…

  19. Adequation between environmental shifts and human speciations during the Plio-PleistoceneAdéquation entre changements environnementaux et spéciations humaines au Plio-Pléistocène

    NASA Astrophysics Data System (ADS)

    Zeitoun, Valery

    2000-01-01

    Several synthetic studies provide evidence of faunistic and floristic shifts as consequences of global climatic events around 2.5 and 1.8 Ma. Some authors are more confident in the role played by local tectonic events to explain such a change in terms of vicariance. It is possible that astronomic, tectonic and climatic variations are linked, as the lithosphere is a catalyst for biosphere more or less strong according to the period. The purpose of this paper is to confront independent environmental and anatomical data to try to understand the evolutionary process in hominids, with particular emphasis on species of the genus Homo in East Africa during the Plio-Pleistocene period. Thus the result of a cladistic analysis based on 35 Otus and 468 features of the calvaria is showing the onset of at least four species to describe the grade of Homo habilis, living in East Africa. The radiation is congruent with the contemporaneous environmental shifts around 2.5 Ma, as is also the case for bovids or cercopithecids. Some Homo erectus left Africa when another climatic pulse happened around 1.8 Ma.

  20. Como ayudar a su hijo a tener exito en la escuela, con actividades para ninos entre las edades de 5 a 11 anos (Helping Your Child Succeed in School, with Activities for Children Ages 5 through 11).

    ERIC Educational Resources Information Center

    Paulu, Nancy

    At the heart of the No Child Left Behind Act of 2001 is a promise to raise standards for all children and to help all children meet those standards. This Spanish-language booklet provides information that parents can use to help their child succeed in school. Following an introduction, the second section of the booklet, "The Basics," offers…

  1. Etude par spectrométrie Raman de l'interaction entre un substrat de graphite et un mélange MCl 4H 2 ( M = Si, Ti)

    NASA Astrophysics Data System (ADS)

    Vincent, H.; Ponthenier, J. L.; Vincent, C.; Bouix, J.

    1989-06-01

    Raman spectroscopy has been used in order to optimize the reactive CVD conditions for silicium carbide or titanium carbide deposits on carbon fibers. With this technique 50-nm-thick carbide coatings could be characterized at the surface of the individual filament constituting the carbon fibers. In bulk carbon substrates evidence of degraphitization near the carbon/carbide interface was observed.

  2. Cinématique des failles entre les unités subbétiques pendant le Miocène (secteur central de la cordillère Bétique).

    NASA Astrophysics Data System (ADS)

    Galindo-Zaldívar, Jesús; Ruano, Patricia; Jabaloy, Antonio; López-Chicano, Manuel

    2000-12-01

    The kinematic analysis of the low-angle faults that separate the major units of the Subbetic Zone indicates two main stages of translations. In the first stage, of Burdigalian to basal Tortonian age, the hanging walls moved toward the WSW, and thrusts developed in some sectors of the External Zones. Simultaneously, in the Internal Zones, the activity of extensional detachments shows the same kinematics. In the second stage, affecting up to basal Tortonian rocks, northwest-verging thrusts were active. The Subbetic tectonic units probably underwent relative rotation during the development of these structures.

  3. The Differences between Spoken and Written Grammar in English, in Comparison with Vietnamese (Las Diferencias entre la Gramática Oral y Escrita del Idioma Inglés en Comparación con el Idioma Vietnamita)

    ERIC Educational Resources Information Center

    Thanh, Nguyen Cao

    2015-01-01

    The fundamental point of this paper is to describe and evaluate some differences between spoken and written grammar in English, and compare some of the points with Vietnamese. This paper illustrates that spoken grammar is less rigid than written grammar. Moreover, it highlights the distinction between speaking and writing in terms of subordination…

  4. Etude theorique et experimentale de la correlation entre la resistance aux chocs thermiques et aux chocs mecaniques des materiaux refractaires utilises dans les fours de traitement de l'aluminium

    NASA Astrophysics Data System (ADS)

    Sebbani, Mohamed Jamal Eddine

    2001-07-01

    This work is a theoretical and experimental study of the correlation between the resistance to thermal shock and mechanical shock of refractory materials. The study of thermal shock showed that the Bahr and Hasselman approaches are alike and that they can be combined into a single, more general approach. This generalisation allowed the division of the theoretical models into two groups: the Hasselman model and the Harmuth model. However, neither of these approaches can predict the behaviour of all refractory materials submitted to thermal shock. Therefore, the generalisation of the Hasselman approach is more appropriate for more dense materials while the Hartmuth approach is more appropriate for less dense materials. The influence of porosity on the energy of rupture helped to explain the behaviour of the less dense material. The absence of generalisation in this case allows those parameters that could be correlated with thermal shock resistance to be dependent on the type of refractory. The study of mechanical shock provided a better understanding of this mechanism. The work performed showed, theoretically and experimentally, that the resistance of the refractory materials could be correlated with the parameter s20 Egwof n-22 . This new parameter helped to explain the statistical correlation between the resistance to mechanical impact and the soxRst parameter established in earlier work. The sintering influence, which makes the refractories more resistant to this type of demand, notably by reducing the "n" coefficient, was shown. This part of the study allowed the establishment of equivalence between thermal fatigue and fatigue by mechanical impact. An evaluation of the correlation between the two mechanisms demonstrated, theoretically and experimentally, that the mechanical and thermal demands could only be exceptionally correlated. In the case of thermal shock, which are imposed deformation demands, it is the shorter cracks which are the most dangerous. However, in the case of mechanical shock, which is constraint imposed, it is the longer cracks that are the most harmful. (Abstract shortened by UMI.)

  5. M&S Support to Assessment of Extended Air Defence C2 Interoperability (Soutien M&S de l’evaluation de l’interoperabilite entre le C2 et la defense aerienne elargie)

    DTIC Science & Technology

    2004-05-01

    ANBACIS D-1 D.2 HAPPIE/RIOT D-1 D.3 MATHEW/ ADPIC D-1 D.4 NUSSE D-2 D.5 PEGEM D-2 D.6 VLSTRACK D-2 Appendix E – Exercises E-1 E.1 Cannon Cloud...http://www.pml.tno.nl/en/ts/hazard_area_prediction.html D.3 MATHEW/ ADPIC MATHEW/ ADPIC The Mass Consistent Wind Field (MATHEW) is a mass-consistent...wind field model that provides three-dimensional winds to the Atmospheric Diffusion Particle in Cell ( ADPIC ) model. ADPIC provides graphical plots of

  6. Como ayudar a su hijo durante los primeros anos de la adolescencia: Para los padres con ninos entre las edades de 10 a 14 anos (Helping Your Child through Early Adolescence: For Parents of Children from 10 through 14).

    ERIC Educational Resources Information Center

    Paulu, Nancy

    Recognizing that parents and families can greatly influence the development of their 10- through 14-year-olds, this Spanish-language booklet is part of a national effort to provide parents with the latest research and practical information to help them support their children both at home and in school. The booklet is organized in 13 sections…

  7. La Mamora, charnière entre la Meseta et le Rif : son importance dans l'évolution géodynamique post-paléozoïque du Maroc

    NASA Astrophysics Data System (ADS)

    Zouhri, Lahcen; Lamouroux, Christian; Buret, Christophe

    The Mamora area (Morocco) is located in the northern part of the Meseta and the southern part of Rharb. The recent formations (Mesozoic to Quaternary) lie unconformably on a Paleozoic basement. This study based on hydrogeological, sedimentological, drilling data and seismic reflection profiles interpretation, proposes new interpretations of geodynamical evolution of this area particularly in terms of tectonic patterns. The most ancient formations recognized in this region are Paleozoic schists and quartzites in the Tiflete sector. They represent the basement of the basin on which Triassic conglomerates and red mudstones associated with basalts lie unconformably. Jurassic and Cretaceous sediments are limestones and marls. The Mio-pliocene formations are open marine blue marls. Plio-quaternary sediments are limestones and sands containing gravel and pebbles. Miocene to Pliocene blue marls facies corresponds to deep marine marls (bathyal as indicated by planctonic foraminifers) with an attributed age from upper Miocene 〚9〛 to middle Pliocene 〚7〛. A facies distribution map of the top of the blue marls has been realized where four main facies - conglomerate, shelly sandstone, limestone and marls - indicate a major regression in the Mamora basin. The datation of the formations was mostly realized by Wernli 〚22, 23, 24, 25〛, and Cirac 〚10〛. In the Mamora area, Hercynian faults show two main structural directions, N020°E-N040°E (Agadir-Rabat), N120°E (Rabat-Tiflète) 〚5〛, and a new major Hercynian fault (K2S). The seismic profiles have been studied between Sidi Slimane and Sidi Yahia area, to illustrate the structure of the Mamora, and to replace it, in the geodynamical evolution. The seismic reflection lines and drilling data show that the eastern Mamora was subdivided into two sectors : i) the southern sector is affected by Hercynian faults which create horsts and grabens in the Paleozoic. Mio-Pliocene formations infill these depressions and are covered by Quaternary sediments ; ii) the northern sector is constituted by various formations : 1. Paleozoic formation as basement covered by autochthonous Mesozoic to Miocene, 2. Prerifain nappes (marls and evaporites), 3. Mio-Plio-Pleistocene formations as subautochthonous to autochthonous. These two sectors are separated by a major fault (K2S). On the other hand, in the occidental Mamora, the facies distribution and the Plio-Pleistocene thickening seem to be induced by faults with a NE-SW and NW-SE trends which affect the Paleozoic basement. Then, between the Meseta domain and the septentrional Rharb basin, two major Hercynian initially dextral shear zones, Rabat-Tiflete and K2S, have been recognised. During the Atlantic Ocean opening, they are probably senestral shear zones. At the same time the subsidence in Rharb basin is active, major action of these faults is normal. Therefore, Mamora represents a real hinge between stable Meseta and unstable septentrional Rharb basin.

  8. Le « Harcèlement Entre Pairs » À L'école élémentaire Tchèque: Une Question D'interprétation

    NASA Astrophysics Data System (ADS)

    Kohout-Diaz, Magdalena

    2008-07-01

    "PEER BULLYING" IN CZECH PRIMARY SCHOOLS: A QUESTION OF INTERPRETATION—This article questions adults' reports of an alleged increase in peer bullying at Czech primary schools. A survey on the school climate revealed that the pupils see it as a generally positive one and consider their relationships to be good. However, compared to France, their behaviour towards the adults is aggressive and rude. This apparent contradiction is linked to a conflict between the educational norms carried over from the old regime (behavioural control and conformism) and the new socio-economic paradigms (critical thinking and free enterprise). Public opinion, the media and a number of scientifically-questionable studies have drawn attention to the phenomenon of peer bullying ( šikana), and applied an imported concept to describe it ( school bullying). This makes it possible to avoid questioning the part played by the adults, the school and the researchers; justify segregating pupils from socially, culturally or economically disadvantaged backgrounds (Roma); and facilitate the country's socio-economic inclusion in the European Union by highlighting educational problems about whose nature there is general agreement.

  9. Análisis y determinación de movimientos propios de cúmulos abiertos entre 200 y 500 pc basados en el catálogo astrométrico UCAC2

    NASA Astrophysics Data System (ADS)

    de Biasi, M. S.; Orellana, R. B.

    We present mean absolute proper motions of five open clusters based on the UCAC2 catalogue up to magnitud R=16.0, for distances between 200 and 500 pc from the Sun. The mean proper motions of the clusters and membership probablity of individual stars were obtained from both position and proper motion data by applying the Vasilevskis-Sanders method modified by the stellar areal density. The clusters analysed are Alessi 1, Collinder 135, Collinder 140, NGC 6633 and Roslund 5. FULL TEXT IN SPANISH

  10. Histoire d’un itinéraire épidémiologique entre le Burkina Faso et la Côte d’Ivoire : le cas des foyers de maladie du sommeil de Koudougou

    PubMed Central

    Kiendrébéogo, D.; Kambiré, R.; Jamonneau, V.; Lingué, K.; Solano, P.; Courtin, F.

    2012-01-01

    Dans la première moitié du XXème siècle, alors que la Haute-Volta (actuel Burkina Faso) subissait une terrible épidémie de maladie du sommeil, l’administration coloniale française a orchestré des déplacements massifs de populations de la Haute-Volta vers la Côte d’Ivoire, pour exploiter le territoire. Cela a conduit à la mise en place de villages de colonisation Mossi en zone forestière ivoirienne, comme ceux de Koudougou, issus de l’une des régions les plus peuplées de Haute-Volta, mais aussi l’une des plus touchées par la maladie du sommeil. Depuis 2000, au Burkina Faso, c’est dans le district sanitaire de Koudougou que sont dépistés passivement le plus grand nombre de trypanosomés en provenance de Côte d’Ivoire. Qui sont-ils ? Où habitent-ils au Burkina Faso ? D’où viennent-ils de Côte d’Ivoire ? Après avoir retracé l’histoire épidémiologique des villages de Koudougou au Burkina Faso et en Côte d’Ivoire, nous avons recherché les trypanosomés dépistés passivement depuis 2000 dans le district sanitaire de Koudougou au Burkina Faso. Au total, dix trypanosomés ont été enquêtés. Le processus de propagation de la maladie du sommeil dans l’espace ivoiro-burkinabé a été mis en évidence et des zones à risque de la maladie identifiées dans ce même espace. PMID:23193525

  11. Fiches pratiques: Vous et vos conversations; Lire le journal autrement; Detourner les titres; Entre Brel et Rimbaud (Practical Ideas: You and Your Conversations; Reading the Newspaper Differently; Deviating Titles; and Between Brel and Rimbaud).

    ERIC Educational Resources Information Center

    Bertrand, Denis; And Others

    1990-01-01

    Four ideas for language classroom activities are presented, including an exercise in oral discourse analysis, a newspaper-reading activity using the holistic approach, the use of slight phonological changes to turn a title into a joke or play on words, and an exercise looking at varieties of language style. (MSE)

  12. Une correlation entre les rendements radiochimiques en hydrogène et les énergies de liaisons calculées pour des familles d'alkènes, d'amines et d'alcools liquides

    NASA Astrophysics Data System (ADS)

    Graffeuil, Marcel; Girouy, Christiane; Grob, Robert; Casanovas, Joseph; Labarre, Jean-Fracçois; Mathieu, Jacques

    A quantum calculation of bond energies for some alkenes, amines and alcohols in the ground state allows the hydrogen yield obtained on irradiation of such molecules to be interpreted. This yield increases normally with the length of saturated chain for alkanes, alkenes and amines, in contrast with alcohols. The behaviour of alcohols could not be interpreted, even when hydrogen bonds, which characterize these alcohols in the liquid state, were included in quantum calculations.

  13. [The EMECAM project: a discussion of the results in the participating cities. Estudio Multicéntrico Español sobre la Relación entre la Contaminación Atmosférica y la Mortalidad].

    PubMed

    Ballester Díez, F; Sáez Zafra, M; Pérez-Hoyos, S; Daponte Codina, A; Bellido Blasco, J B; Cañada Martínez, A; Abad Díez, J M; Pérez Boíllos, M J; Alonso Fustel, M E; Taracido Trunk, M; Aguinaga Ontoso, I; Guillén Pérez, J J; Ordóñez Iriarte, J M; Saurina Canals, C; Tenías Burillo, J M

    1999-01-01

    This article draws a comparison and provides a discussion of the findings resulting from the local analyses of the 14 cities participating in the EMECAM Project. An analysis is made of the time series related to mortality, pollutants (particles in suspension, SO2, NO2, O3 and CO), temperature and other factors taken from records of public institutions. By using Poisson autoregressive regression, an estimate has been made of the short-term relationship between the number of deaths and the air pollution indicators in each one of the following cities: Barcelona, metropolitan area of Bilbao, Cartagena, Castellón, Gijón, Huelva, Madrid, Pamplona, Seville, Oviedo, Valencia, Vigo, Vitoria and Saragossa. The findings reveal the air pollution figures in our country to be similar to those of other European cities. The levels of the different pollutants point toward road traffic as being the main source of most of this pollution. A relationship has been found between the mortality and different pollutants in most cities, although the results are not homogeneous among the cities and show variability in the different causes under study. In some cities, especially in those having smaller populations, there have been no findings providing any evidence of a relationship, or the findings themselves are not highly consistent. The meta-analysis will provide estimates for all of the cities as a whole and will allow the possibility of making a more clear-cut assessment of the time lag impact of air pollution on the mortality. Worthy of special mention is the participation in this project of public health officers as actively involved researchers.

  14. O Imperio como Argumento: Um Contraponto entre Joaquim Nabuco e o Bispo D. Jose Mauricio da Rocha (The Empire as Argument: A Counterpoint between Joaquim Nabuco and the Bishop Dom Jose Mauricio da Rocha).

    ERIC Educational Resources Information Center

    Marson, Izabel Andrade

    1998-01-01

    Dialogs with the previous article establishing a comparative analysis between the political acceptance present in the reactionary speech of Dom Jose Mauricio da Rocha and in the monarchist words of Joaquim Nabuco. (PA)

  15. The Relationship Between Non-Governmental Organizations and the Canadian Forces: A Pilot Study (La relation entre les organisations non-gouvernementales et les forces canadiennes: Une etude pilote)

    DTIC Science & Technology

    2010-09-01

    organizations from which to draw participants include a broader range of NGOs, international organizations (IOs), as well as other government departments (OGDs...such as the Royal Canadian Mounted Police (RCMP), the Canadian International Development Agency (CIDA), and the Department of Foreign Affairs and... International Trade (DFAIT), paid contractors, and other development groups all of which are involved in the theatre of operations. Within the CF, a

  16. Highlighting the Impacts of North-South Research Collaboration among Canadian and Southern Higher Education Partners (Principaux impacts des collaborations de recherche Nord-Sud entre les partenaires des etablissements d'enseignement superieur du Canada et du Sud)

    ERIC Educational Resources Information Center

    Association of Universities and Colleges of Canada, 2006

    2006-01-01

    The Association of Universities and Colleges of Canada (AUCC) felt it was timely to create an academic forum in which university researchers have the opportunity to engage with their peers and relevant stakeholders and document the impacts of their North-South research collaboration in a peer-reviewed publication. The Association achieved this by…

  17. Effets de compétition entre les phénomènes de dopage et d'endommagement dans les polymères électroactifs implantés

    NASA Astrophysics Data System (ADS)

    Moliton, A.; Moreau, C.; Lucas, B.; Friend, R. H.; Froyer, G.

    1994-09-01

    From a general band scheme appropriate for electroactive polymers, we explain how each of the two main processes (damage or doping) induced by ion implantation can play a major role. DC conductivity, thermopower and AC conductivity studied versus temperature for varying implantation parameters allow us to confirm our interpretations : damage is produced by high implantation parameters in which case transport phenomena of p type occur mainly in degenerate states near the Fermi level, whereas low implantation parameters induce doping in polaronic bands characterized by thermally activated processes, although effects due to the existence of defects can appear, especially at low temperature. Compensation effects, described by a Kubo-Greenwood formula applied to the thermoelectric power, give a consistent explanation of the successive behaviours. Moreover, we present a general interpretation of the p type conductivity behaviour observed in intrinsic polymers. En utilisant le schéma de bandes des polymères électroactifs, nous expliquons les raisons pour lesquelles au cours de l'implantation ionique dans ces matériaux les deux principaux, processus générés (le dopage et les endommagements) sont à même de jouer un rôle important. Les mesures de conductivité continue, de pouvoir thermoélectrique et de conductivité alternative ont conforté nos interprétations : de forts paramètres d'implantation génèrent des endommagements qui induisent des phénomènes de transports de type p principalement dans les états dégénérés situés au voisinage du niveau de Fermi, alors que de faibles paramètres d'implantation favorisent le dopage dans les bandes polaroniques caractérisées par des processus thermiquement activés bien que la présence de défauts puisse se faire sentir notamment à basse température. Des effets de compensation, décrits à partir de la formule de Kubo-Greenwood appliquée au pouvoir thermoélectrique, fournissent une explication satisfaisante des différents comportements rencontrés. De plus, nous présentons une interprétation générale de l'origine de la conductivité de typep rencontrée dans les polymères intrinsèques.

  18. Relaciones hidrogeologicas y medioambientales entre el mar mediterraneo, El saladar y el acuifero de agua amarga (provincia de alicante). Incidencia de las explotaciones de las desaladoras de alicante I Y II Y medidas correctoras

    NASA Astrophysics Data System (ADS)

    Manteca, Ivan Alhama

    The Agua Amarga coastal aquifer, located in the south of Alicante province, has been subjected to successive anthropogenic actions: salt works activity (1925-1975) and water withdrawal to supply the Alicante I and II desalination plants (since 2003). These interventions have influenced the salinity and the flow regime. Due to the existence of a salt marsh of ecological interest linked to the aquifer, the 'Mancomunidad de los Canales del Taibilla' (agency responsible for the desalination plants) designed a piezometric network for monitoring piezometry and electrical conductivity (in operation since May 2008). Soil humidity and piezometrics levels have been recovered by means of a seawater pouring programme over the salt marsh (since December 2009),which represents the third anthropic intervention. In this memoria, we investigate the Agua Amarga coastal aquifer to develop a physical conceptual model. Firstly, the study area is characterized in relation to climatology, geology, geomorphology and hydrogeology, using published information, describing, in addition, the desalination plants catchment system. Next, based on hydrogeological studies prior to the start up of the desalination plants, the aquifer is characterized: type, lithology, hydraulic parameters, thickness, surface extension, etc. Water quantity relating to rainfall, water withdrawal and pourings over the salt marsh, have been integrated in conjunction with data from monthly piezometric campaigns. In adittion, in order to gain insight into the groundwater mixing processes, chemical and isotope analyses were carried out on meteoric water and groundwater samples taken at different locations. The results were used to elaborate a conceptual physical model and a water budget. As an extension tool to understand processes and assess aquifer management, a 3-D fluid-flow and solute-transport model is designed with SEAWAT. Also, the 2-D physical characterization of scenarios with seawater intrusion and salt flats is presented. Finally, after describing ecological values of the salt marsh, the effect of the seawater pouring programme on aquifer piezometry and salinity is studied, assessing the possibility of applying this pilot scheme to other scenarios.

  19. Teacher Collaboration Praxis: Conflicts, Borders, and Ideologies from a Micropolitical Perspective (La práctica colaborativa entre docentes: conflictos, fronteras e ideologías desde una perspectiva micropolítica)

    ERIC Educational Resources Information Center

    Keranen, Nancy; Encinas Prudencio, Fátima

    2014-01-01

    This paper looks at a feature of teacher collaboration within Achinstein's (2002) micropolitics of collaboration but from an intrapersonal perspective. Results discussed feature issues of conflict, borders, and ideologies within each participating teacher rather than between teachers. Unresolved or unacknowledged intrapersonal conflict might lead…

  20. Peer Interaction: A Social Perspective towards the Development of Foreign Language Learning (Interacción entre pares: una perspectiva social hacia el desarrollo del aprendizaje en una lengua extranjera)

    ERIC Educational Resources Information Center

    Gómez Lobatón, July Carolina

    2011-01-01

    This pedagogical innovation aims at discovering new ways of interaction that go beyond the unidirectional relationship that is presented in the classrooms most of the times. The innovation considers peers to be active agents in the construction of knowledge and proposes new ways to arrange groups in the classroom so that the arrangement can…

  1. Predictive Relationship between Humane Values of Adolescents Cyberbullying and Cyberbullying Sensibility (Relaciones predictivas entre los valores humanos de los adolescentes, el acoso cibernético y la sensibilidad al acoso cibernético)

    ERIC Educational Resources Information Center

    Dilmac, Bülent; Yurt, Eyüp; Aydin, Mustafa; Kasarci, Ismail

    2016-01-01

    Introduction: Cyberbullying has been more common than the traditional bullying in recent years. As with the traditional bullying, humane values likely to explain the reason why adolescents tend to bully via cyber-means. Cyber-bullying behaviors also reasoned by adolescents' sensibility towards it. This study investigates the predictive…

  2. Vinculos Entre la Capacitacion de Docentes y el Desarrollo de Materiales Educativos Impresos--Anotaciones para Una Discusion (Links between In-service Teacher Training and the Development of Printed Educational Aids).

    ERIC Educational Resources Information Center

    Valle, Victor M.

    Textbooks and other printed materials will be ever present, in the foreseeable future, as major aids for teaching and learning processes. New textbooks imply new guidelines for teachers, and the ways to handle such guidelines demand teacher training. This paper explains some links between inservice teacher training programs and the development of…

  3. Potencial de Seqüestro de Carbono Atmosférico entre Diferentes Cultivares de Milho (Zea mays L.) sob Condiç o de Déficit Hídrico

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There is a question concerning the role of agricultural practices on carbon sequestration enhancement. By producing biomass with agricultural crops and adding this residue to soil, it should act on the mitigation process of the greenhouse effect, especially CO2. The objectives of this study were to ...

  4. Etude de la Recharge de l’Accumulateur Plomb-Acide Red Camel Entre +20 C et -30 C (A Study of the Rechargeability of the Red Camel Lead-Acid Battery between +20 C and -30 C).

    DTIC Science & Technology

    1983-10-01

    Pour un voltage limite imposg de 14.3 V et une dur~e de charge de 7 heures, les rendements en courant (Rc) et en gnergie fi1ectrique (Re), calcul~s en...pour le d~marrage des v~hicules A moteur . Cependant en dega de -20*C, on assiste A une diminution importante des performances en dicharge rapide...rficup~ration de la capaciti dana le calcul des rendements en courant et en finergie filectrique. CONDUITE DES ESSAIS Lors de toul lea easais, le courant

  5. Conséquences de l'interaction entre préférences pour l'habitat et quantité de ressources trophiques sur les communautés d'insectes coprophages (Coleoptera: Scarabaeoidea)

    NASA Astrophysics Data System (ADS)

    Kadiri, Nassera; Lobo, Jorge M.; Lumaret, Jean-Pierre

    Between the years 1988 and 1995, five dung beetle communities were studied in combined conditions of habitat and trophic resources. Investigations were conducted on La Viale experimental farm, in the Causse Méjean, southern France. At each site, dung beetle traps of standard design were set up simultaneously. In most situations, the vegetation cover acted like a filter that allowed the penetration of several ubiquitous but fundamentally open-habitat dung beetle species into forest, at the same time drastically limiting their numbers. Open and close sites differed significantly, open and grazed sites showing the highest richness, abundance and biomass in beetles. Both in open and close sites, a significant increase in trophic resources led to an increase of the diversity and evenness. However in open-habitat, when resources were too abundant, few species were favoured and the diversity and evenness decreased. In forest-habitat, a continuous grazing during several years (increase in resources) modified the dung beetle community, with a high increase in the numbers and biomass of beetles but a smaller increase in richness. Numbers in forest habitat readjusted to the level observed in open habitat with equivalent trophic resources. Similarly to what was observed in open pastures, the forest communities were able to adjust their numbers to the level of available trophic resources. Resources were a more active variable than the structure of habitat on the dung beetle communities. Important resources go against the filter effect which allows the penetration under cover of open-habitat species. Changes were slow (several years), and forest communities remained weak. The poverty of forest habitats is discussed: This scarcity in species is probably due to long time human activities in Europe which drastically reduced forest habitats and developped grazing, favouring generalist and heliophilous open-habitat dung beetle species. Actual grassland communities in Europe are richer than their homologous communities in North America. Conversely, in North America where human activities are recent, the true forest dung beetle communities are still diverse and richer than grassland communities.

  6. Vinculacion Entre La Educacion Y El Mundo Del Trabajo: Informe Final. (Coloquio Regional Caracas, Venezuela, Septiembre 2-6, 1985) = The Linkage Between Education and Employment: Final Report of the Regional Colloquium (Caracas, Venezuela, September 2-6, 1985).

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Santiago (Chile). Regional Office for Education in Latin America and the Caribbean.

    At this regional colloquium 13 papers were presented concerning the relationship between education and employment. Themes addressed were: (1) the transformation of the workplace by technology and science; (2) the future of the disadvantaged population as a consequence of the technological revolution; and (3) the resulting changes in the education…

  7. Pushing Too Little, Praising Too Much? Intercultural Misunderstandings between a Chinese Doctoral Student and a Dutch Supervisor (¿Empujando muy poco, felicitando demasiado? Malentendidos interculturales entre un estudiante de doctorado Chino y un supervisor Holandés)

    ERIC Educational Resources Information Center

    Hu, Yanjuan; van Veen, Klaas; Corda, Alessandra

    2016-01-01

    To understand the challenges and their causes in interactions between Western supervisors and international doctoral students, we conducted a self-study of our experiences as a Chinese international student and her Dutch supervisor during her doctoral research project. We found the supervisor and the student to differ in their expectations of the…

  8. A Comparison of Chinese and Colombian University EFL Students Regarding Learner Autonomy (Comparación entre estudiantes universitarios de inglés chinos y colombianos con respecto a su autonomía como aprendices)

    ERIC Educational Resources Information Center

    Buendía Arias, Ximena Paola

    2015-01-01

    This research seeks to gain deeper understanding of learner autonomy in English as a Foreign Language students from different cultures through the identification and analysis of similarities and differences between Chinese and Colombian students from two public universities: Tianjin Foreign Studies University in China and Universidad Surcolombiana…

  9. Corrélation stratigraphique entre les unités oligo-miocènes de Tunisie centrale et le NumidienStratigraphic correlation between the Numidian formation (North Tunisia) and Oligo Miocene deposits of central Tunisia.

    NASA Astrophysics Data System (ADS)

    Yaich, Chokri; Hooyberghs, Herman-Jean-François; Durlet, Christophe; Renard, Maurice

    2000-10-01

    Sedimentologic and biostratigraphic studies from the Oligocene to Middle Miocene deposits, exposed in central and north Tunisia, lead to a sequence stratigraphy subdivision of these deposits into several third order depositional sequences. The north-south correlations show that the detrital quartz pebbles appeared much later in central Tunisia (within a 'fluvial-deltaic' complex), than in the Numidian in northern Tunisia (characterised by deep submarine channels filled with high density turbidites). Thus, in central Tunisia, the Fortuna Formation (from Rupelian to Lower Burdigalian) could not have supplied Numidian sands from the Saharan region as has been proposed by several authors.

  10. Triterpenoidal Saponins: Bioactive Secondary Metabolites from Zygophyllum coccineum

    DTIC Science & Technology

    2011-01-22

    of the publishers only Planta Medica Journal of Medicinal Plant and Natural Product Research www.thieme.de/fz/plantamedica l www.thieme-connect.com...insecticidal activity of compounds 1, 3, 5, 6, and 9. Amin E et al. Triterpenoidal Saponins: Bioactive… Planta Med Letters Th is is a co py of th e...Amin E et al. Triterpenoidal Saponins: Bioactive… Planta Med Letters Th is is a co py of th e au th or ʼs pe rs on al re pr in t Th is is a co py of th

  11. Effects of Self-Esteem and Mortality Salience on Attitudes Toward Canadian Security: Exploring the Significance of Implicit-Explicit and Personal-Collective Distinctions (Les Effets de L’Estime de soi et de la Pregnance de la Mort sur les Attitudes a L’Egard de la Securite Canadienne: Analyse de L’Importance des Distinctions entre Implicite et Explicite d’une Part et entre Personnel et Collectif D’Autre Part)

    DTIC Science & Technology

    2011-04-01

    worthy that worldview is considered to be, and can be considered a reflection of one’s social identity. According to Tajfel (1982), social identity is... discrimination among high explicit self-esteem individuals. Personality and Social Psychology Bulletin, 31, 693-702. Jordan, C. H., Spencer, S. J., Zanna, M...R., Dyrenforth, P. S., & Hagiwara, N. (2006). Why are attributions to discrimination interpersonally costly? A test of system- and group-justifying

  12. Noninvasive Detection of Lactate as a Biomarker of Response Using Spectral-Selective Multiple Quantum Editing Sequence (SS-SelMQC)

    DTIC Science & Technology

    2010-05-01

    Tuli Rakesh. And Khetrapal, C.L. (2010). Metabolic and Histopathological alterations of Jatropha Mosoic begamovirus infected Jatropha Curcus L. by HR-MAS NMR Spectroscopy and Magnetic Resonace Imaging. Planta . 232: 85-93

  13. Eco-hydrology: Groundwater flow and site factors in plant ecology

    NASA Astrophysics Data System (ADS)

    Klijn, Frans; Witte, Jan-Philip M.

    proporciona unas condiciones de vida adecuadas que permiten el crecimiento de una especie o una comunidad botánica. En un material inicialmente homogéneo, el flujo subterráneo gravífico influencia las condiciones del emplazamiento variando la distribución espacial de los nutrientes y de otros agentes químicos relevantes. En especial, el flujo ascendente puede producir y mantener una serie de condiciones que son esenciales para algunas especies y comunidades de plantas relativamente raras. La especial atención hacia este fenómeno ha dado lugar a una cooperación entre ecologistas e hidrogeólogos y a la aparición de una nueva disciplina - eco-hidrología -, en la frontera de los dos campos científicos. En Holanda, se ha usado una clasificación sencilla de tipos de agua para crear una base de datos, a nivel nacional, de lugares donde la presencia de flujos ascendentes pueda ser de interés ecológico. El análisis de correspondencias entre esta base de datos y los tipos de plantas existentes muestra que en los suelos arenosos pobres del Pleistoceno los flujos ascendentes explican la presencia de algunas especies y comunidades de plantas. Por el contrario, en las llanuras fluviales y pólders, con suelos más arcillosos, la influencia de estos flujos es despreciable frente a la relativa a las propias características de los suelos. Se concluye que las especies botánicas se pueden usar como indicadores de la presencia de flujo ascendente sólo en campañas preliminares, pero que el método presenta grandes limitaciones para su extensión a casos generales.

  14. Uvby-B Photometry of High Velocity Stars. Photometric Parallaxes and Preliminary Kinematic Results

    NASA Astrophysics Data System (ADS)

    Schuster, W. J.

    1990-11-01

    RESUMEN. Se han explorado dos metodos para la determinaci6n de paralajes fotometricos usando fotometrfa uv - . Estos metodos dependen de las relaciones estandar de Crawford (1975) y de Olsen (1984) y de colores y magnitudes sinteticas de VandenBerg y Bell (1985). Ambos metodos incluyen una correcci6n evolucionaria de forma f6c0. Se calculan las distancias para las 711 estrellas de alta velocidad y pobres en metales en el catalogo uvby-p de Schuster y Nissen (1988). Se comparan estas con las distancias de Sandage y Fouts (1987) y Laird, Carney y Latham (1988) para las estrellas en comtfin. Tambien son aplicables nuestros metodos a estrellas de paralaje. En general las comparaciones son satisfactorias y las sistematicas son despreciables o pequefias. Las distancias finales de nuestras 711 estrellas se aplican a un numero de problemas cinematicos. Se estudian algunos diagramas interesantes, tales como el diagrama de energia de Toomre y el diagrama V(rot) versus [Fe/H]. ABSTRACT Two methods for the determination of parallaxes using uvbyP photometry are being explored. These methods depend upon the standard relations of Crawford (1975) and of Olsen (1984) and upon synthetic colors and magnitudes of VandenBerg and Bell (1985). Both include an evolutionary correction of the form f6c0. Distances are calculated for the 711 high-velocity and metal-poor stars in the uvby-P catalogue of Schuster and Nissen (1988). These are compared to the distances of Sandage and Fouts (1987) and Laird, Carney, and Lathain (1988) for stars in common. Also our methods are applied to parallax stars. In general the comparisons are good with negligible or small systematic differences. The final distances of our 711 stars are applied to a number of kinematical problems. Several interesting diagrams are studied, sucl as Toomre energy diagram and the plot of V(rot) versus [Fe/H]. Key words: DISTANCES - PHOTOMETRY - STARS-POPULATION II

  15. The Armed Forces and the Fight against Drug-Trafficking in Honduras (Las FUERZAS ARMADAS EN EL COMBATE AL NARCOTR FICO EN HONDURAS)

    DTIC Science & Technology

    2013-06-13

    estén abrumados, por su propia solicitud . Nuestra Constitución prevé que la Policía Nacional pueda otorgar poderes a nuestras Fuerzas Armadas, a pedido...aprovechado desde el punto de vista que le dan empleo con altas 1Secretaría de Defensa de Honduras, Libro Blanco de la Defensa Nacional Tegucigalpa...comprensible dado que las solicitudes se han realizado a la distancia. Delimitaciones La investigación cubre aspectos e incidentes en particular de

  16. Fotometría CCD de dos cúmulos abiertos con estrellas WR: NGC 6231 y TR 27

    NASA Astrophysics Data System (ADS)

    Baume, G.; Vázquez, R. A.; Feinstein, A.

    Se ha realizado fotometría CCD en los filtros UBVI del sistema de Cousins en las zonas de los cúmulos abiertos NGC 6231 y Trumpler 27, llegando hasta aproximadamente magnitud V=19. Ambos objetos presentan estrellas WR en su proximidad. Se ha re-determinado el Eb-v en esas direcciones, asi como la distancia a la que se encontrarían los cúmulos y sus edades por medio de un ajuste de la ZAMS y de un conjunto de isocronas respectivamente.

  17. Estudio de la influencia de la refrigeracion con aire de forma natural e inducida en el comportamiento de instalaciones fotovoltaicas

    NASA Astrophysics Data System (ADS)

    Mazon Hernandez, Rocio

    panels are analysed to compare and select the best configuration. The presented research provides a deep knowledge of how they work as well as information and results for an improvement in future designs of building integrated photovoltaic systems. Este estudio se centra en analizar la influencia negativa de la temperatura en la produccion electrica de paneles fotovoltaicos al estar emplazados sobre cubierta de acero, como sucede en naves industriales y sobre un invernadero. Se estudian diferentes configuraciones que permitan refrigerar los paneles, reduciendo su temperatura y mejorar su rendimiento. Para abordar este problema, se han construido dos instalaciones experimentales, fieles a plantas solares en funcionamiento. Una instalacion engloba dos paneles fotovoltaicos sobre estructura fija al suelo. Uno de los paneles esta integrado sobre una superficie paralela y metalica. Entre ambas superficies existe un espacio que posibilita circular aire, permitiendo refrigerar el panel por conveccion natural, o conveccion forzada impulsando el aire con un ventilador. El otro panel, libre por su cara posterior y se ha considerado de referencia. Se ha estudiado el comportamiento del panel integrado sobre cubierta para diferentes secciones de aire y velocidades inducidas, comparandolo con el panel de referencia. Se ha desarrollado un modelo experimental que nos permite determinar la temperatura del panel en funcion de las variables que influyen en su refrigeracion. Adicionalmente, se han analizado los datos de una planta solar en funcionamiento, con paneles de igual caracteristicas, obteniendo correlaciones entre la temperatura del panel y las variables electricas y comparandolos con las obtenidas en la instalacion experimental. La segunda instalacion experimental reproduce parte de una instalacion solar sobre un invernadero, formada por cuatro paneles fotovoltaicos colocados sobre el plastico del invernadero, existiendo un canal divergente entre ambas superficies. Se estudia la

  18. Hydraulic properties and scale effects investigation in regional rock aquifers, south-western Quebec, Canada

    NASA Astrophysics Data System (ADS)

    Nastev, M.; Savard, M. M.; Lapcevic, P.; Lefebvre, R.; Martel, R.

    subterránea sucede en los primeros metros de la secuencia rocosa. A escala regional se puede aplicar la aproximación equivalente de medios porosos. Las medidas de la conductividad hidrálica fueron comparadas con la escala de las pruebas del acuífero, expresadas con el volumen investigado del acuífero. Se desarrolló un proceso de interpolación simple para la conductividad hidráulica de campo, con base en la distancia entre las medidas de campo y los volúmenes probados del acuífero. La distribución regional de la conductividad hidráulica, para las unidades acuíferas fracturadas mayores, indica que la dolomita es más permeable mientras que la arenisca y las rocas cristalinas son las unidades menos permeables. L'article présente les propriétés hydrauliques d'un aquifère régional rocheux qui ont été mesurées dans le cadre d'un projet concernant l'estimation de la ressource en eau de la plaine de St. Lawrence située dans la partie sud-ouest de Quebec. Affin de comprendre le comportement de l'aquifère tant à l'échelle de fracture qu'à l'échelle régionale on a mené des investigations hydrauliques en utilisant des essais differents. À l'échelle locale l'écoulement est déterminé en principal par le système des fractures. Les résultats des essais d'injection au niveau constant ont montré une tendence de décroissance de la conductivité hydraulique avec la profondeur ce qui indique que l'écoulement ait lieu en principal dans les premiers mètres de la structure rocheuse. À l'échelle règionale on peut appliquer l'approche de milieux poreux equivalent. Les mesures de conductivité hydraulique ont été corrélées avec l'échelle des essais de pompage exprimée en termes de volume investigué de l'aquifère. On a mis au point une méthode simple d'interpolation pour le champ de la conductivité hydraulique basée sur la distance entre les essais in situ et les volume investigués de l'aquifère. La distribution règionale de la conductivit

  19. Phytoreovirus-like sequences isolated from salivary glands of the glassy-winged sharpshooter Homolodisca vitripennis (Hemiptera: Cicadellidae)

    SciTech Connect

    Katsar, C.S.; Hunter, W.B. . E-mail: Whunter@ushrl.ars.usda.gov; Sinisterra, X.H.

    2007-03-15

    GWSS occurs, such as in southern California, will provide a better understanding of the potential role of the GWSS as a disease vector in the spread of phytoreoviruses and other plant pathogens. The sequences have been deposited in NCBI database under the accession numbers (EF058280) for GWSS-V1, WHSg013C11 and (EF058281) for GWSS-V2, WHSg024H02. (author) [Spanish] Dos fragmentos de 610 y 839 pares de bases fueron aislados apartir de una genoteca de expresion derivada de las glandulas salivales del cucarron de las alas cristalinas (GWSS), Homalodisca vitripennis, Germar 1821 (syn. H. coagulata) el cual es vector de la enfermedad de Pierce de las uvas. Los resultados de alineamiento utilizando BLASTX, BLASTP y el analisis filogenetico utilizando PAUP indicaron que los fragmentos de DNA estaban relacionado de manera mas cercana a viruses en la familia Reoviridae, genero Phytoreovirus, y especificamente a los virus del enanismo del arroz (RDV) y al virus del tumor de las grietas (WTV). El cucarron de las alas cristalinas es un saltahoja que se alimenta no solo del xilema sino tambien del floema y del mesofilo. Saltahojas del genero Agallian, son los principales vectores de WTV, el cual infecta el floema de plantas dicotiledoneas tumoraciones en las hojas y en las raices. WTV es transmitido por saltahojas y es el unico reovirus que es capaz de infectar tanto tejidos del xilema como del floema. El comportamiento alimentario del GWSS y su amplio rango de hospederos que incluye pastos y plantas herbaceas y lenosas podria proveer la interaccion entre estos dos organismos facilitando la adquisicion y tranmision de fitoreovirus por el GWSS. Un adecuado monitoreo de el incremento en la expansion de reoviruses en cultivos de gramineas asociados al los vinedos en donde GWSS ocurre en regiones tales como el sur de California, y en general el sur de los Estados Unidos, podria proveer un mejor entendimiento del papel del GWSS como vector de fitoreovirus y otros patogenos de plantas

  20. The quiescent center and the stem cell niche in the adventitious roots of Arabidopsis thaliana.

    PubMed

    Rovere, Federica Della; Fattorini, Laura; Ronzan, Marilena; Falasca, Giuseppina; Altamura, Maria Maddalena

    2016-05-03

    Adventitious rooting is essential for the survival of numerous species from vascular cryptogams to monocots, and is required for successful micropropagation. The tissues involved in AR initiation may differ in planta and in in vitro systems. For example, in Arabidopsis thaliana, ARs originate from the hypocotyl pericycle in planta and the stem endodermis in in vitro cultured thin cell layers. The formation of adventitious roots (ARs) depends on numerous factors, among which the hormones, auxin, in particular. In both primary and lateral roots, growth depends on a functional stem cell niche in the apex, maintained by an active quiescent center (QC), and involving the expression of genes controlled by auxin and cytokinin. This review summarizes current knowledge about auxin and cytokinin control on genes involved in the definition and maintenance of QC, and stem cell niche, in the apex of Arabidopsis ARs in planta and in longitudinal thin cell layers.

  1. The Understanding of Astronomy Concepts by Students from Basic Education of a Public School. (Spanish Title: El Entendimiento de Conceptos de Aastronmía Por Los Alumnos de Educación Básica en Una Escuela Pública.) O Entendimento de Conceitos de Astronomia Por Alunos da Educação Básica: O Caso de Uma Escola Pública Brasileira

    NASA Astrophysics Data System (ADS)

    Iria Machado, Daniel; dos Santos, Carlos

    2011-07-01

    We present the results obtained in a research on the comprehension of basic astronomical concepts, in which 561 students from fifth grade middle school to third grade high school of a public school of the city of Foz do Iguaçu (Brazil) took part. A test with 20 multiple-choice questions was applied to indentify the most common conceptions expressed by the students. This test was elaborated based on the literature about misconceptions and covered the following topics: the day-night cycle; the time zones; the seasons of the year; the phases of the Moon; the movement of the Moon; the apparent movement of the Sun in the celestial sphere; the eclipses; the dimensions and distances in the Universe; the brightness of the stars and its observation from Earth. Though a small progress was verified in the proportion of scientifically acceptable answers when comparing the eighth grade of middle school to the fifth, and the third grade of high school to the first, there was an overall predominance of alternative conceptions regarding most of the explored subjects, which persisted up to the last year of secondary school. The comparison to data found in this research made in other socio-cultural contexts revealed, in many aspects, similar notions and difficulties revealed by the students. Se presentan los resultados de una investigación sobre la comprensión de conceptos astronómicos básicos, en la cual participaron 561 estudiantes que cursaban entre el quinto grado de la enseñanza primaria y el tercer año de la enseñanza secundaria de una escuela pública de la ciudad de Foz do Iguaçu (Brasil). Se utilizó un test de 20 preguntas de opción múltiple para identificar las concepciones más comunes expresadas por los estudiantes. Este instrumento de recolección de datos se desarrolló en base a la literatura sobre las concepciones alternativas y trató los siguientes temas: el ciclo día-noche, los husos horarios, las estaciones del año, las fases de la Luna, el

  2. Geostatistical mapping of leakance in a regional aquitard, Oak Ridges Moraine area, Ontario, Canada

    NASA Astrophysics Data System (ADS)

    Desbarats, A. J.; Hinton, M. J.; Logan, C. E.; Sharpe, D. R.

    2001-01-01

    'imperméable, dans le but d'évaluer l'importance relative des processus d'alimentation des aquifères inférieurs. À partir d'une vaste base de données constituée par les mesures faites dans les puits et contenant des informations à la fois certaines et incertaines, la méthode de simulation par indicateur séquentiel est utilisée pour créer des cartes d'épaisseur de l'imperméable et de probabilité d'existence des fenêtres. Ces cartes peuvent être utilisées pour mettre en évidence les aquifères de chenaux et pour identifier les zones potentielles de recharge des aquifères inférieurs. Les conductivités hydrauliques sont modélisées à partir de données clairsemées en supposant que leur gamme de corrélation est beaucoup plus faible que le pas de la grille. La drainance à l'échelle des blocs est obtenue par accroissement du niveau d'échelle des valeurs nodales basé sur les champs de conductivité simulée et des épaisseurs. À partir de l'hypothèse d'écoulement dans l'aquifère, l'accroissement d'échelle est réalisé en faisant une moyenne arithmétique spatiale. Des histogrammes et des cartes de drainance avec accroissement d'échelle montrent que les hétérogénéités associées aux fenêtres dans l'imperméable constituent l'effet le plus important dans l'organisation des écoulements souterrains régionaux. Resumen. El Till de Newmarket forma un acuitardo de extensión regional que separa dos sistemas acuíferos principales en la zona de Greater Toronto (Canadá). El till está horadado, y a veces completamente erosionado, por una red de canales rellenos de arena y grava. Estos constituyen acuíferos productivos y, localmente, conexiones de alta permeabilidad entre sistemas acuíferos. El goteo a través del till puede ser fundamental en ciertos lugares. Este estudio investiga la variabilidad espacial del goteo desde el acuitardo con el fin de establecer la importancia relativa de los procesos de recarga hacia los acuíferos inferiores. Se ha

  3. Membrane heredity and early chloroplast evolution.

    PubMed

    Cavalier-Smith, T

    2000-04-01

    Membrane heredity was central to the unique symbiogenetic origin from cyanobacteria of chloroplasts in the ancestor of Plantae (green plants, red algae, glaucophytes) and to subsequent lateral transfers of plastids to form even more complex photosynthetic chimeras. Each symbiogenesis integrated disparate genomes and several radically different genetic membranes into a more complex cell. The common ancestor of Plantae evolved transit machinery for plastid protein import. In later secondary symbiogeneses, signal sequences were added to target proteins across host perialgal membranes: independently into green algal plastids (euglenoids, chlorarachneans) and red algal plastids (alveolates, chromists). Conservatism and innovation during early plastid diversification are discussed.

  4. Results from Second Round of Remediated Nitrate Salt Surrogate Formulation and Testing

    SciTech Connect

    Brown, Geoffrey Wayne; Leonard, Philip; Hartline, Ernest Leon; Tian, Hongzhao

    2016-04-04

    High Explosives and Technology (M-7) completed the second round of formulation and testing of Remediated Nitrate Salt (RNS) surrogates on March 17, 2016. This report summarizes the results of the work and also includes additional documentation required under test plan PLAN-TA9-2443 Rev B, "Remediated Nitrate Salt (RNS) Surrogate Formulation and Testing Standard Procedure", released February 16, 2016. All formulation and testing was carried out according to PLAN-TA9-2443 Rev B. Results from the first round of formulation and testing were documented in memorandum M7-16-6042, "Results from First Round of Remediated Nitrate Salt Surrogate Formulation and Testing."

  5. Spatial patterns of carbon, biodiversity, deforestation threat, and REDD+ projects in Indonesia

    PubMed Central

    Murray, Josil P; Grenyer, Richard; Wunder, Sven; Raes, Niels; Jones, Julia PG

    2015-01-01

    del Carbono, la Biodiversidad, la Amenaza de Deforestación y los Proyectos REDD+ en Indonesia Resumen Actualmente hay preocupación por que las Emisiones Reducidas de la Deforestación y Degradación del Bosque (REDD+, en inglés) puedan fallar en la entrega de co–beneficios potenciales de la biodiversidad si se enfocan en áreas de alto carbono. Exploramos los traslapes espaciales entre los stocks de carbono, la biodiversidad, las amenazas proyectadas de deforestación y la ubicación de proyectos REDD+ en Indonesia, un país tropical a la vanguardia del desarrollo REDD+. Para la biodiversidad, reunimos datos sobre la distribución de vertebrados terrestres (hábitats de anfibios, mamíferos, aves y reptiles) y plantas (modelos de distribución de especies para ocho familia). Después investigamos la congruencia entre las diferentes medidas de la riqueza de la biodiversidad y los stocks de carbono en la escala nacional y sub–nacional. Finalmente mapeamos proyectos REDD+ activos e investigamos la densidad de carbono y la riqueza potencial de la biodiversidad y modelamos presiones de deforestación dentro de estos bosques en relación con áreas protegidas y no protegidas. Hubo poco traslape interno entre los diferentes hotspots (el 10% más rico de las celdas) de riqueza de especies. Tampoco hubo congruencia espacial consecuente entre los stocks de carbono y las medidas de la biodiversidad: una correlación negativa débil en la escala nacional enmascaró las relaciones altamente variables y no-lineales isla por isla. Los proyectos REDD+ actuales estuvieron ubicados preferencialmente en áreas con una riqueza total de especies y con una riqueza de especies amenazadas más altas pero con densidades de carbono más bajas que en las áreas protegidas y los bosques sin protección. Aunque un cuarto del área total de estos proyectos REDD+ está bajo una presión de deforestación relativamente alta, la mayoría del área REDD+ no lo está. Por lo menos en Indonesia

  6. Global Professional Identity in Deterretorialized Spaces: A Case Study of a Critical Dialogue between Expert and Novice Nonnative English Speaker Teachers (Identidad profesional global en espacios desterritorializados: un estudio de caso de los diálogos críticos entre profesores de inglés no nativos)

    ERIC Educational Resources Information Center

    Guerrero Nieto, Carmen Helena; Meadows, Bryan

    2015-01-01

    This study analyzes the online, peer-peer dialogue between two groups of nonnative English-speaking teachers who are attending graduate programs in Colombia and the United States. Framed by the theoretical concepts of critical pedagogy and global professional identity, a qualitative analysis of the data shows that their expert vs. novice roles…

  7. Human Relations Activities for the Single Parent To Develop More Effective Parent/Child Relations. Bilingual Guide = Actividades sobre relaciones humanas para ayudar al padre-soltero o madre-soltera a desarrollar una relacion efectiva entre padre e hijo Guia bilingue.

    ERIC Educational Resources Information Center

    Peterson, Susan

    Written in English and Spanish, this bilingual guide offers 10 activities which single parents can use to improve their relationships with their children. Objectives of activities include: (1) developing children's responsibility for work tasks in the home; (2) improving sibling relationships; (3) discussing emergencies with children; (4)…

  8. Relation entre la mastication et la qualité de l'alimentation des aînés de l'Étude longitudinale québécoise sur la nutrition et le vieillissement (NuAge).

    PubMed

    Cusson, Valérie; Payette, Hélène; Caron, Christian; Shatenstein, Bryna; Gaudreau, Pierrette

    2015-12-01

    The objective of this study was to assess the relationship between the quality of mastication and dietary intake among community-dwelling older adults (67-84 years). A secondary analysis of data collected upon entry of participants in the NuAge study was effected. Perceived masticatory efficiency (n=1793) was measured using a questionnaire inspired by the Oral Health Impact Profile. Direct assessment of masticatory efficiency was carried out in a subsample (n=94), using a validated clinical test (Swallowing Threshold Test Index, 0-100%). Dietary intake was calculated from three non-consecutive 24h recalls. Mean scores indicate good perceived masticatory efficiency (Men:26.22±0.19/28;Women:25.81±0.21/28), but weak assessed efficiency (Men:60.8% [CI(57.3-64.2)]; Women:61.2% [CI(57.7-64.7)]). Multivariate regression analysis, adjusting for confounding variables (age, sex, schooling, living alone, income, smoking, chronic conditions, functional autonomy), showed that masticatory efficiency (measured or perceived) was not associated with intake of fruits/vegetables, protein, vitamin C or folic acid.

  9. Contamination par le nickel et d'autres métaux lors de la manipulation des pièces de monnaie comparaison entre francs français et eurosContamination by nickel and other metals resulting from the manipulation of coins comparison between French francs and euros

    NASA Astrophysics Data System (ADS)

    Fournier, P.-G.; Govers, T. R.; Fournier, J.; Abani, M.

    The introduction of the euro has revived the interest for potential nickel allergies. We show that the handling of a single used nickel coin releases an average of 0.45 μg of nickel, 0.26 μg of copper and 0.08 μg of zinc, whereas a euro coin composed of nickel alloys releases 0.25 μg of nickel, 1.3 μg of copper and 0.17 μg of zinc. These levels of contamination, favored by friction during handling, differ from those obtained on the basis of the EN 1811 test applicable to solubilisation in artificial sweat. The evaluation of allergy risks following the introduction of the euro may require the consideration of potential synergies between the metals mentioned above. To cite this article: P.-G. Fournier et al., C. R. Physique 3 (2002) 749-758.

  10. Executive Summary of The Use of Educational Software in Adult Literacy Programs: A Comparison of Integrated Learning Systems and Stand-Alone Software=Resume du Rapport Intitule: L'Utilisation de Didacticiels dans les Programmes d'Alphabetisation des Adultes: Une Comparaison Entre les Systemes Integres d'Apprentissage et les Logiciels Autonomes.

    ERIC Educational Resources Information Center

    Millar, Diane

    The pros and cons of integrated learning systems (ILS) and stand-alone software for adult literacy instruction were examined in a comparative analysis that focused on their use in adult literacy programs in Canada. The comparison focused on the following variables: achievement gains in reading; changes in students' attitudes toward computers;…

  11. En sus marcas--Listos--A leer! Para los cuidadores de ninos pequenos: Actividades de lenguaje para la primera infancia y ninez entre el nacimiento y los 5 anos. El reto: A leer, America! (Ready--Set--Read! For Caregivers: Early Childhood Language Activities for Children from Birth through Age Five. America Reads Challenge).

    ERIC Educational Resources Information Center

    Department of Education, Washington, DC.

    This Ready--Set--Read Kit includes an activity guide for caregivers, a 1997-98 early childhood activity calendar, and an early childhood growth chart. The activity guide presents activities and ideas that caregivers (family child care providers and the teachers, staff, and volunteers in child development programs) can use to help young children…

  12. Tensions entre rationalité technique et intérêts politiques : l’exemple de la mise en œuvre de la Loi sur les agences de développement de réseaux locaux de services de santé et de services sociaux au Québec

    PubMed Central

    Contandriopoulos, D.; Hudon, Raymond; Martin, Elisabeth; Thompson, Daniel

    2013-01-01

    Sommaire L’objet de cet article est constitué par les processus décisionnels entourant la mise en œuvre de la Loi sur les agences de développement de réseaux locaux de services de santé et de services sociaux (Loi 25). Nous entendons mettre en lumière les stratégies des groupes ou institutions de diverses natures qui ont fait valoir leurs préférences et ont tenté, avec un succès inégal, d’influencer les décisions relatives à cette réforme majeure de la structure du système de santé québécois. Au plan théorique, nous nous appuyons principalement sur les modèles d’analyse du lobbying qui, depuis les travaux fondateurs de Milbrath (1960, 1963), présentent cette pratique comme un processus fondamental d’échange d’information. Selon les données colligées dans les retranscriptions d’entrevues, les stratégies observées correspondent effectivement aux caractéristiques constitutives du lobbying et, dans quelques situations, à celles du patronage. La combinaison de ces divers éléments révèle que la mise en œuvre de la Loi 25 s’avère être avant tout un processus proprement politique. Ainsi, furent relégués au second plan les arguments techniques qui composaient initialement les objectifs de la Loi. PMID:23509412

  13. [The EMECAM protocol: an analysis of the short-term effect of air pollution on mortality. Estudio Multicéntrico Español sobre la Relación entre la Contaminación Atmosférica u la Mortalidad].

    PubMed

    Pérez-Hoyos, S; Sáez Zafra, M; Barceló, M A; Cambra, C; Figueiras Guzmán, A; Ordóñez, J M; Guillén Grima, F; Ocaña, R; Bellido, J; Cirera Suárez, L; López, A A; Rodríguez, V; Alcalá Nalvaiz, T; Ballester Díez, F

    1999-01-01

    The aim of this study is to Mortality show the protocol of analysis which was set out as part of the EMECAM Project, illustrating the application thereof to the effect of pollution has on the mortality in the city of Valencia. The response variables considered will be the daily deaths rate resulting from all causes, except external ones. The explicative variables are the daily series of different pollutants (black smoke, SO2, NO2, CO, O3). As possible confusion variables, weather factors, structural factors and weekly cases of flu are taken into account. A Poisson regression model is built up for each one of the four deaths series in two stages. In the first stage, a baseline model is fitted using the possible confusion variables. In the second stage, the pollution variables or the time legs thereof are included, controlling the residual autocorrelation by including mortality time lags. The process of fitting the baseline model is as follows: 1) Include the significant sinusoidal terms up to the sixth order. 2) Include the significant temperature or temperature squared terms with the time lags thereof up to the 7th order. 3) Repeat this process with the relative humidity. 4) Add in the significant terms of calendar years, daily tendency and tendency squared. 5) The days of the week as dummy variables are always included in the model. 6) Include the holidays and the significant time lags of up to two weeks of flu. Following the reassessment of the model, each one of the pollutants and the time lags thereof up to the fifth order are proven out. The impact is analyzed by six-month periods, including interaction terms.

  14. El juego entre el nacimiento y los 7 anos: Un manual para ludotecarias. Investigacion-accion sobre la Familia y la Primera Infancia. UNESCO Sector de Educacion Monografia (Play between Birth and 7 Years: A Manual for Preschool Teachers. Action Research in Family and Early Childhood. UNESCO Education Sector Monograph).

    ERIC Educational Resources Information Center

    Grellet, Carolina

    The purpose of this Spanish-language manual is to help preschool teachers better understand theoretical concepts related to children's play. Following a preface that discusses UNESCO's activities related to early childhood education, the two main sections of the manual discuss children's play between 0-2 years and 2-7 years. Each section includes…

  15. Construyendo un Puente entre la Educacion del Dotado y el Mejoramiento Escolar Integral. Resumen. La Serie de Decision Basada en la Investigacion (Building a Bridge between Gifted Education and Total School Improvement. Summary. Talent Development Research-Based Decision Making Series).

    ERIC Educational Resources Information Center

    Renzulli, Joseph S.

    This monograph addresses the role of gifted education in total school improvement by describing three service delivery components (the Total Talent Portfolio, Curriculum Modification Techniques, and Enrichment Learning and Teaching) and several organizational components of the Schoolwide Enrichment Model (SEM). The report describes how the SEM can…

  16. How To Talk to Your Doctor (and Get Your Doctor To Talk to You!). An Educational Workshop on Doctor Patient Communication = Como Hablarle a su Doctor (iY que su doctor le hable a usted!). Un seminario educativo sobre la comunicacion entre el doctor y el paciente.

    ERIC Educational Resources Information Center

    Baylor Coll. of Medicine, Houston, TX.

    This workshop, written in both English and Spanish, focuses on improving communication between physician and patient. In the workshop, the trainers will talk about "building bridges" between patient and doctor by understanding the doctor's role and his/her duty to the patient. According to the workshop, a person's doctor should…

  17. Les Differences dans l'emploi de la construction possessive "N1"+"DE"+"N2" entre le XIX et le XX s. dans la prose litteraire francaise (Differences in the Use of the Possessive Construction "N1"+"de"+"N2" between 19th and 20th Century French Literature).

    ERIC Educational Resources Information Center

    Roglic, Vera

    1991-01-01

    Study looks at frequency of noun1 + de + noun2 possessive in French prose. Researchers used chi-square and Pearson test to analyze results of syntagmatic possessive construction in works by Hugo, Stendahl, Balzac, Zola, Gide, Malraux, St. Exupery, and Sartre. Findings indicate that syntagmatic genitive possessive is more commonly found in…

  18. Mitigation of Disagreement in Peer Review among L2 Learners and Native Speakers in a College Writing Class (Mitigación del Impacto de las Opiniones de Desacuerdo en el Proceso de Revisión por Pares entre Estudiantes de una Segunda Lengua y Hablantes Nativos en una Clase de Escritura a Nivel Universitario)

    ERIC Educational Resources Information Center

    Christoffersen, Katherine O'Donnell

    2015-01-01

    Peer review is now a commonplace practice in process-oriented writing instruction. A crucial aspect of peer review is assessing another classmate's work, which encompasses the act of disagreement. Given its prevalence in the classroom, it is necessary to analyze how L2 learners mitigate disagreement in the context of peer review with other L2…

  19. En sus marcas--Listos--A leer! Para las familias: Actividades de lenguaje para la primera infancia y ninez entre el nacimiento y los 5 anos. El reto: A leer, America! (Ready--Set--Read! For Families: Early Childhood Language Activities for Children from Birth through Age Five. America Reads Challenge).

    ERIC Educational Resources Information Center

    Department of Education, Washington, DC.

    This Ready--Set--Read Kit includes an activity guide for families, a 1997-98 early childhood activity calendar, and an early childhood growth wallchart. The activity guide presents activities and ideas that families (adults who have nurturing relationships with a child--a mother, father, grandparent, other relative, or close friend) can use to…

  20. Efectos Especiales de Anclaje (Estudio sobre Regresiones de Juicios Condicionales). Parte 1: Distincion entre Efectos Aditivos y Efectos Multiplicativos en el Fenomeno de Anclaje (Special Effects of Anchoring (Study on Regression of Conditional Judgements) Part 1: Distinction Between Additive Effects and Multiplicative Effects in the Phenomenon of Anchoring). Publication No. 17.

    ERIC Educational Resources Information Center

    Lopez Alonso, A. O.

    A linear relationship was found between judgements given by 160 subjects to 7 objects presented as single stimuli (alpha judgements) and judgements given to the same objects presented with a condition (gamma judgements). This relationship holds for alpha judgements and the gamma judgements that belong to a family of constant stimulus and varying…

  1. Linking: Today's Libraries, Tomorrow's Technologies. Report of the Bibliographic and Communications Network Pilot Project. Canadian Network Papers Number 7 = Le Lien entre les Bibliotheques d'Aujourd'hui et les Technologies de Demain. Rapport du Projet Pilote du Reseau de Services Bibliographiques et de Communications. Documents sur les Reseaux Canadiens Numero 7.

    ERIC Educational Resources Information Center

    National Library of Canada, Ottawa (Ontario).

    A pilot project was conducted from May 1980 to November 1983 to test the application of iNet--a decentralized, packet-switched telecommunications network--to bibliographic data interchange in Canada. The principal components of the project were participation of the Bibliographic Common Interest Group (BCIP), a group of libraries with stand-alone,…

  2. Burkholderia phymatum strains capable of nodulating Phaseolus vulgaris are present in Moroccan soils.

    PubMed

    Talbi, C; Delgado, M J; Girard, L; Ramírez-Trujillo, A; Caballero-Mellado, J; Bedmar, E J

    2010-07-01

    Phylogenetic analysis of 16S rRNA, nodC, and nifH genes of four bacterial strains isolated from root nodules of Phaseolus vulgaris grown in Morocco soils were identified as Burkholderia phymatum. All four strains formed N(2)-fixing nodules on P. vulgaris and Mimosa, Acacia, and Prosopis species and reduced acetylene to ethylene when cultured ex planta.

  3. Burkholderia phymatum Strains Capable of Nodulating Phaseolus vulgaris Are Present in Moroccan Soils ▿

    PubMed Central

    Talbi, C.; Delgado, M. J.; Girard, L.; Ramírez-Trujillo, A.; Caballero-Mellado, J.; Bedmar, E. J.

    2010-01-01

    Phylogenetic analysis of 16S rRNA, nodC, and nifH genes of four bacterial strains isolated from root nodules of Phaseolus vulgaris grown in Morocco soils were identified as Burkholderia phymatum. All four strains formed N2-fixing nodules on P. vulgaris and Mimosa, Acacia, and Prosopis species and reduced acetylene to ethylene when cultured ex planta. PMID:20472732

  4. The Irish Potato Famine Pathogen Phytophthora infestans Translocates the CRN8 Kinase into Host Plant Cells

    PubMed Central

    van Damme, Mireille; Bozkurt, Tolga O.; Cakir, Cahid; Schornack, Sebastian; Sklenar, Jan; Jones, Alexandra M. E.; Kamoun, Sophien

    2012-01-01

    Phytopathogenic oomycetes, such as Phytophthora infestans, secrete an arsenal of effector proteins that modulate plant innate immunity to enable infection. We describe CRN8, a host-translocated effector of P. infestans that has kinase activity in planta. CRN8 is a modular protein of the CRN effector family. The C-terminus of CRN8 localizes to the host nucleus and triggers cell death when the protein is expressed in planta. Cell death induction by CRN8 is dependent on its localization to the plant nucleus, which requires a functional nuclear localization signal (NLS). The C-terminal sequence of CRN8 has similarity to a serine/threonine RD kinase domain. We demonstrated that CRN8 is a functional RD kinase and that its auto-phosphorylation is dependent on an intact catalytic site. Co-immunoprecipitation experiments revealed that CRN8 forms a dimer or multimer. Heterologous expression of CRN8 in planta resulted in enhanced virulence by P. infestans. In contrast, in planta expression of the dominant-negative CRN8R469A;D470A resulted in reduced P. infestans infection, further implicating CRN8 in virulence. Overall, our results indicate that similar to animal parasites, plant pathogens also translocate biochemically active kinase effectors inside host cells. PMID:22927814

  5. Isolongifolenone: A Novel Sesquiterpene Repellent of Ticks and Mosquitoes

    DTIC Science & Technology

    2009-01-01

    forWindows step by step: a simple guide and reference. Allyn & Bacon, Bos- ton, MA. Gerberg, E. J., D. P. Barnard, and R. A.Ward. 1994. Manual for mosquito... Planta Med. 63: 158Ð 160. He, K., L. Zeng, G. Shi, G.-X. Zhao, J. F. Kozlowski, and J. L. McLaughlin. 1997b. Bioactive compounds from Taiwa- nia

  6. Polyhedral Oligomeric Silsesquioxane-Functionalized Perfluorocyclobutyl Aryl Ether Polymers: An Overview of the Synthesis and Properties of Polyhedral Oligomeric Silsesquioxanes (POSS) Functionalized with Perfluorocyclobutyl (PFCB) Aryl Ether Polymer Blends and Copolymers (Preprint)

    DTIC Science & Technology

    2007-10-17

    Contact Analyzer System or a Rime-Hart 100-00 115 Goniometry. Liquid drops were either automatically or manually dispensed with 8 10 L drop sizes...C.; Barthlott, W. Ann. Bot. 1997, 79, 677. 2. Barthlott, W.; Nenhuis, C. Planta 1997, 202, 1. 3. Sun, T.; Feng, L.; Gao, X.; Jian, L. Acc

  7. Probing Pseudomonas syringae host interactions using metatranscriptomics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Transcriptome analyses during the interaction of plants and pathogens can be used to provide insights into molecular mechanisms of plant resistance as well as the mechanisms used by bacteria to adapt to hosts and cause disease. We performed a dual in planta RNA-Seq experiment to profile RNA expressi...

  8. Infection of Helicoverpa armigera by endophytic Beauveria bassiana colonizing tomato plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A novel endophytic strain of Beauveria bassiana was isolated from leaf tissue of a wild tomato plant. This strain and two B. bassiana strains previously isolated from soil were evaluated for their ability to endophytically colonize tomatoes and subsequent in planta efficacy against Helicoverpa armig...

  9. Evolutionary relationships of Metazoa within the eukaryotes based on molecular data from Porifera.

    PubMed Central

    Schütze, J; Krasko, A; Custodio, M R; Efremova, S M; Müller, I M; Müller, W E

    1999-01-01

    Recent molecular data provide strong support for the view that all metazoan phyla, including Porifera, are of monophyletic origin. The relationship of Metazoa, including the Porifera, to Plantae, Fungi and unicellular eukaryotes has only rarely been studied by using cDNAs coding for proteins. Sequence data from rDNA suggested a relationship of Porifera to unicellular eukaryotes (choanoflagellates). However, ultrastructural studies of choanocytes did not support these findings. In the present study, we compared amino acid sequences that are found in a variety of metazoans (including sponges) with those of Plantae, Fungi and unicellular eukaryotes, to obtain an answer to this question. We used the four sequences from 70 kDa heat-shock proteins, the serine-threonine kinase domain found in protein kinases, beta-tubulin and calmodulin. The latter two sequences were deduced from cDNAs, isolated from the sponge Geodia cydonium for the phylogenetic analyses presented. These revealed that the sponge molecules were grouped into the same branch as the Metazoa, which is statistically (significantly) separated from those branches that comprise the sequences from Fungi, Plantae and unicellular eukaryotes. From our molecular data it seems evident that the unicellular eukaryotes existed at an earlier stage of evolution, and the Plantae and especially the Fungi and the Metazoa only appeared later. PMID:10081159

  10. RNA Interference Based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Over the past decade RNA interference (RNAi) technology has emerged as a successful tool not only for functional genomics, but in planta expression of short interfering RNAs (siRNAs) could offer potential for insect pest management. Insects feeding exclusively on plant sap depend on osmotic pressure...

  11. 800,000 gal/d reverse-osmosis system treats seawater for boiler-feed makeup

    SciTech Connect

    Pang, A.W.; Kinstler, W.I.

    1980-01-01

    The Planta Centro Station for CADAFE is intended to demonstrate that seawater reverse osmosis is a viable water-treatment process for the power industry. Reverse osmosis offers to the industry a flexible modular design, easy to operate and economically favorable. It has established itself as a prime competitor to the more-conventional evaporator process. 4 refs.

  12. The dynamics of apoplast phenolics in tobacco leaves following inoculation with bacteria

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study demonstrates that the accumulation of apoplastic phenolics is stimulated in planta in response to bacterial inoculation. Past studies have shown that levels of extracellular phenolics are elicited in plant cell suspensions in response to bacteria, and that tomato plants infected with vir...

  13. Draft Genome Sequence of Burkholderia cenocepacia Strain 869T2, a Plant-Beneficial Endophytic Bacterium

    PubMed Central

    Ho, Ying-Ning

    2015-01-01

    An endophytic bacterium, Burkholderia cenocepacia 869T2, isolated from vetiver grass, has shown its abilities for both in planta biocontrol and plant growth promotion. Its draft genome sequence was determined to provide insights into those metabolic pathways involved in plant-beneficial activity. This is the first genome report for endophytic B. cenocepacia. PMID:26564046

  14. Draft Genome Sequence of Burkholderia cenocepacia Strain 869T2, a Plant-Beneficial Endophytic Bacterium.

    PubMed

    Ho, Ying-Ning; Huang, Chieh-Chen

    2015-11-12

    An endophytic bacterium, Burkholderia cenocepacia 869T2, isolated from vetiver grass, has shown its abilities for both in planta biocontrol and plant growth promotion. Its draft genome sequence was determined to provide insights into those metabolic pathways involved in plant-beneficial activity. This is the first genome report for endophytic B. cenocepacia.

  15. Extracellular Genomic DNA Mediates Enhancement of Xylella fastidiosa Biofilm Formation in Vitro

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Xylella fastidiosa (Xf) produces extracellular DNA in PD3 liquid medium. This extracellular DNA could enhance biofilm formation, a factor in successful establishment of Xf in planta. The relative amounts of extracellular DNA were positively correlated with planktonic growth and biofilm formation in ...

  16. Functional, structural and phylogenetic analysis of domains underlying the Al-sensitivity of the aluminium-activated malate/anion transporter, TaALMT1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    TaALMT1 (Triticum aestivum Aluminum Activated Malate Transporter) is the founding member of a novel gene family of anion transporters (ALMTs) that mediate the efflux of organic acids. A small subgroup of root-localized ALMTs, including TaALMT1, is physiologically associated with in planta aluminum (...

  17. The parA resolvase performs site-specific genomic excision in Arabidopsis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We have designed a site-specific excision detection system in Arabidopsis to study the in planta activity of the small serine recombinase ParA. Using a transient expression assay as well as stable transgenic plant lines, we show that the ParA recombinase is catalytically active and capable of perfo...

  18. Advances in flavanoid glycosyltransferase research: integrating recent findings with long-term citrus studies

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Flavonoid glycosides are required for a number of crucial roles in planta and have the potential for development in a variety of agricultural, medicinal, and biotechnological applications. A number of recent advancements have been made in characterizing glycosyltransferases, the enzymes that are res...

  19. 40 CFR 174.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... amended (7 U.S.C. 136 et seq.). Food includes articles used for food or drink by humans or other animals... plant-incorporated protectants, means an organism classified using the 5-kingdom classification system of Whittaker in the kingdom Plantae. This includes, but is not limited to, bryophytes such as...

  20. 40 CFR 174.3 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... amended (7 U.S.C. 136 et seq.). Food includes articles used for food or drink by humans or other animals... plant-incorporated protectants, means an organism classified using the 5-kingdom classification system of Whittaker in the kingdom Plantae. This includes, but is not limited to, bryophytes such as...

  1. There's No Such Thing as a One-Celled Plant or Animal.

    ERIC Educational Resources Information Center

    Kaveski, Sharon; And Others

    1983-01-01

    Considers the classification of organisms into five kingdoms based on evidence from molecular biology and microscopic techniques. Divides organisms into Protoctista, Monera, Fungi, Plantae, and Animalia, discussing characteristics of prokaryotes and eukaryotes as related to division of the kingdoms--prokaryotes in kingdom Monera and eukaryotes in…

  2. 40 CFR 174.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... amended (7 U.S.C. 136 et seq.). Food includes articles used for food or drink by humans or other animals... plant-incorporated protectants, means an organism classified using the 5-kingdom classification system of Whittaker in the kingdom Plantae. This includes, but is not limited to, bryophytes such as...

  3. 40 CFR 174.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... amended (7 U.S.C. 136 et seq.). Food includes articles used for food or drink by humans or other animals... plant-incorporated protectants, means an organism classified using the 5-kingdom classification system of Whittaker in the kingdom Plantae. This includes, but is not limited to, bryophytes such as...

  4. Identification of microdomains involved in association of "Arabidopsis" Ca(2+)/H(+) exchangers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In planta, high capacity tonoplast Ca2+/H+ antiport is mediated in part by a family of CAtion Exchangers (CAX). Each CAX can be divided into two weakly homologous halves (N- and C-) at the negatively charged loop between transmembrane (TM) 6 and TM7. Some CAX halves (N+C) co-expressed in yeast cells...

  5. The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.

    PubMed

    van Damme, Mireille; Bozkurt, Tolga O; Cakir, Cahid; Schornack, Sebastian; Sklenar, Jan; Jones, Alexandra M E; Kamoun, Sophien

    2012-01-01

    Phytopathogenic oomycetes, such as Phytophthora infestans, secrete an arsenal of effector proteins that modulate plant innate immunity to enable infection. We describe CRN8, a host-translocated effector of P. infestans that has kinase activity in planta. CRN8 is a modular protein of the CRN effector family. The C-terminus of CRN8 localizes to the host nucleus and triggers cell death when the protein is expressed in planta. Cell death induction by CRN8 is dependent on its localization to the plant nucleus, which requires a functional nuclear localization signal (NLS). The C-terminal sequence of CRN8 has similarity to a serine/threonine RD kinase domain. We demonstrated that CRN8 is a functional RD kinase and that its auto-phosphorylation is dependent on an intact catalytic site. Co-immunoprecipitation experiments revealed that CRN8 forms a dimer or multimer. Heterologous expression of CRN8 in planta resulted in enhanced virulence by P. infestans. In contrast, in planta expression of the dominant-negative CRN8(R469A;D470A) resulted in reduced P. infestans infection, further implicating CRN8 in virulence. Overall, our results indicate that similar to animal parasites, plant pathogens also translocate biochemically active kinase effectors inside host cells.

  6. Cigarrillos de mentol | Smokefree Español

    Cancer.gov

    ¿Qué es el mentol? El mentol es una sustancia encontrada naturalmente en las plantas de menta, tales como la menta fresca y la hierbabuena. Deja una sensación refrescante y se usa frecuentemente para aliviar el dolor y la irritación leve, y para prevenir infecciones [1].

  7. The HDF1 histone deacetylase gene is important for conidiation, sexual reproduction, and pathogenesis in Fusarium graminearum

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Head blight caused by Fusarium graminearum is an important disease of wheat and barley. Its genome contains chromosomal regions with higher genetic variation and enriched for genes expressed in planta, suggesting a role of chromatin modification in the regulation of infection-related genes. In a pre...

  8. Maize seedling blight induced by Fusarium verticillioides: accumulation of fumonisin B1 in leaves without colonization of the leaves

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fusarium verticillioides produces fumonisin mycotoxins during the colonization of maize, and fumonisin B1 (FB1) production is necessary for manifestation of maize seedling blight disease. The objective of this study was to assess the in planta occurrence of fumonisin, fungal colonization, and disea...

  9. Molecular characterization of an endornavirus from Cucumis spp

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Endornaviruses infect hosts in the kingdoms Plantae, Fungi and Chromista. They are efficiently transmitted vertically and generally do not induce visible symptoms. In this investigation high molecular weight dsRNA, representing the genome of an endornavirus, was isolated from an unknown melon (Cucum...

  10. Trazando el brazo de Vela-Puppis

    NASA Astrophysics Data System (ADS)

    Giorgi, E. E.; Vázquez, R. A.; Baume, G. L.; Seggewiss, W.; Feinstein, A.

    Presentamos resultados preliminares basados en análisis fotométricos UBVI para 9 cúmulos abiertos ubicados en la zona de Vela-Puppis (α =7h58m y 9h54m, δ =- 28o a -59o) sobre un total de 15 cúmulos previstos. Estos cúmulos no han sido observados previamente, excepto en algunos pocos casos que se cuenta con fotometría y espectroscospía de algunas estrellas. Se han determinado membrecias, excesos de color, distancias y edades. En el futuro, la muestra global permitirá definir mas claramente este brazo y será utilizada para estudiar las funciones iniciales de masa individuales e integradas y compararlas con las obtenidas en observaciones intensivas realizadas en otras zonas de la Galaxia.

  11. La distribución de objetos en el Cinturón de Kuiper y la posible existencia de un nuevo planeta en el Sistema Solar

    NASA Astrophysics Data System (ADS)

    Brunini, A.

    A pesar de que la capacidad observacional no lo limita, no se han descubierto objetos en el Cinturón de Kuiper más allá de las 50 AU. Recientemente, hemos propuesto que un embrión planetario originalmente formado en la región de Urano-Neptuno, y posteriormente migrado hacia distancias mayores a 50-60 AU, puede explicar este hecho. Presentamos simulaciones numéricas de acreción del Sistema Solar exterior que muestran cómo, naturalmente, podriamos esperar que un planeta de este tipo se encuentre actualmente en regiones distantes del sistema solar. Analizamos además la posibilidad de detectarlo con instrumentos actuales.

  12. Nuevos fenómenos en erupciones cometarias

    NASA Astrophysics Data System (ADS)

    Silva, A.

    Se discuten aquí tres procesos físicos novedosos encontrados en la actividad de cometas: 1) El rol de una distribución de granos de hielo como fuente extendida de H2O en la coma, 2) El efecto de una discontinuidad en el plasma cometario, llamada Cometopausa, sobre la excitación del radical OH , y 3) La actividad por erupciones a grandes distancias heliocéntricas (r > 5 AU). Con respecto a 1) y 2), se presentan modelos que ajustan bien con las observaciones. En cuanto a 3), se presentan explicaciones posibles al fenómeno, y se trata el interesante caso de Chirón 2060, basándose en observaciones propias tomadas desde el CASLEO y datos anteriores.

  13. Fotometría y polarimetría de Lynga 1

    NASA Astrophysics Data System (ADS)

    Brusasco, M. A.; Baume, G.; Vázquez, R. A.

    Se presentan los resultados de las observaciones fotométricas CCD-UBVRI hasta magnitud aproximadamente V = 18 y polarimétricas hasta V = 13 en la zona del cúmulo abierto Lynga 1. El mismo se halla en una dirección cercana a un remanente de supernova y fue estudiado previamente por Peterson y FitzGerald (1988) mediante fotometría fotoeléctrica. Se ha redeterminado el enrojecimiento, la distancia y la edad de dicho objeto. Se han hallado además los valores de las pendientes de las funciones de luminosidad y de masa comparándoselas con las de otros cúmulos de edades similares.

  14. Fotometría y polarimetría de Trumpler 21

    NASA Astrophysics Data System (ADS)

    Giorgi, E.; Baume, G.; Vázquez, R. A.

    Se presentan los resultados de las observaciones fotométricas CCD-UBVI hasta aproximadamente magnitud V = 19 y polarimétricas UBVRI hasta V =13 en la región del cúmulo abierto Trumpler 21. El mismo se halla en una dirección cercana al remanente de supernova G307.6-0.3 y fue estudiado previamente por Moffat y Vogt (1973) y por Peterson y FitzGerald (1988) mediante fotometría fotoeléctrica de las estrellas más brillantes. Se ha redeterminado el enrojecimiento, la distancia y la edad de dicho objeto. Se han hallado además los valores de las pendientes de las funciones de luminosidad y de masa comparandoselas con las de otros cúmulos de edades similares.

  15. Observaciones CCD de los cúmulos abiertos Melotte 105, Ruprecht~75 y Pismis 7

    NASA Astrophysics Data System (ADS)

    Ahumada, J.; Lapasset, E.

    Se presentan los resultados preliminares de observaciones CCD en cinco colores (UBVRI) de campos de ~2,5' de radio centrados en los cúmulos abiertos Melotte 105, Ruprecht 75 y Pismis 7. Los datos fueron adquiridos en abril de 1996 con el telescopio de 60 cm de la Universidad de Toronto en Las Campanas (Chile), y el detector PM512 en su modo directo. Se muestran los diagramas color-magnitud y color-color preliminares de los cúmulos. Mediante criterios fotométricos se hace una selección de los posibles miembros. También se determinan enrojecimientos, distancias y edades de los cúmulos.

  16. NASA Astrophysics Data System (ADS)

    Ahumada, J. A.; Lapasset, E.

    Presentamos resultados de observaciones CCD en cuatro colores (BVRI) de áreas de ~ 5' de radio centradas en los cúmulos abiertos NGC 2658 y NGC 5617. Dichas observaciones se llevaron a cabo en los años 1997 y 1998 con el telescopio de 2,15 m del CASLEO y el detector TEK 1024 en su modo directo con reductor focal. En los diagramas color-magnitud preliminares se aprecian secuencias bien definidas; mediante criterios fotométricos, hacemos una selección de los probables miembros. Asimismo se determinan enrojecimientos, distancias, edades y otros parámetros básicos de los cúmulos.

  17. Fotometría CCD en el campo del cúmulo abierto Ruprecht 58

    NASA Astrophysics Data System (ADS)

    Giorgi, E. E.; Vázquez, R. A.; Seggewiss, W.; Ostrov, P.; Solivella, G. R.

    La región de Vela-Puppis contiene varios cúmulos cuyo estudio puede brindarnos información acerca de la cinemática y el estado evolutivo de la población estelar en las zonas externas del disco galáctico. A través de observaciones CCD en los filtros UBV en el campo del cúmulo abierto Ruprecht 58 (α2000= 8h 14m 41s, δ2000=-31o 57'), se han determinado por primera vez los parámetros fundamentales de este cúmulo tales como distancia, enrojecimiento y edad. Se analiza también la estructura de su secuencia principal y la presencia de gigantes y binarias.

  18. Formación estelar y AGNs en los entornos de quasars

    NASA Astrophysics Data System (ADS)

    Coldwell, G.; García Lambas, D.

    En este trabajo utilizamos las galaxias del catálogo 2dF (2dF public 100K data release) y muestras de quasars tomados del catálogo Verón-Cetty & Verón (2001) para estudiar la naturaleza de estas galaxias en los entornos de quasars con redshift en el rango 0.1< z < 0.2. Estudiamos la distribución de índices espectrales, η, de galaxias a distintas distancias proyectadas de quasars y con diferencias de velocidad radial Δ V <= 500 km s-1. Por comparación, realizamos el mismo análisis en una muestra de galaxias random del catálogo 2dF y en una muestra de cúmulos Abell con similar distribución de redshift que los quasars. Los resultados indican que existe una gran fracción de galaxias con fuertes líneas de emisión, eta > 3.5, en los entornos de quasars comparado con la fracción presente en las vecindades de galaxias típicas del 2dF. Analizamos las distribuciones de luminosidad para estas galaxias (eta > 3.5) encontrando un exceso de galaxias mas luminosas que M ˜ -19.5 en las vecindades de quasars, indicativo de la posible presencia de AGNs. Por otro lado, estimamos la tasa de formación estelar promedio para objetos a distintas distancias de quasars, galaxias y cúmulos de galaxias detectando una actividad de formacion estelar significativamente alta dentro de 1.5 Mpc h-1 de quasars con respecto a las galaxias del 2dF. Estos resultados proveen evidencia de un particular entorno de galaxias alrededor de Quasars.

  19. [MEDICAGO SATIVA L: IMPROVEMENT AND NEW APPROACHES OF ITS NUTRITIONAL AND FUNCTIONAL VALUE BY BACTERIAL CO-INOCULATION].

    PubMed

    Martínez, Rosario; Nebot, Elena; Porres, Jesús María; Kapravelou, Garyfallia; Del Moral, Ana; Talbi, Chouhra; Bedmar, Eulogio Jose; López-Jurado, María

    2015-12-01

    Objetivo: estudiar el efecto de la inoculación con Ensifer meliloti y Halomonas maura sobre el crecimiento y el valor nutricional y funcional de la leguminosa Medicago sativa L., cultivada bajo condiciones de salinidad. Método: las plantas de M. sativa se cultivaron con una solución de mezcla de sales CaSO4, MgCl, NaCl and Na- HCO3 y se coinocularon con su rizobio específico y la bacteria H. maura. Se determinaron los parámetros fisiológicos de las plantas, así como el contenido en nitrógeno y minerales, y se llevó a cabo un proceso de digestibilidad in vitro. Resultados: la salinidad ejerció un efecto negativo sobre las plantas; sin embargo, la coinoculación de las mismas incrementó su productividad, el contenido en nitrógeno, minerales totales, Ca y Mg. Además, los parámetros fisiológicos de potencial hídrico y concentración de leghemoglobina se incrementaron. Tanto la salinidad como la coinoculación de las plantas aumentaron la capacidad antioxidante de la leguminosa en los dializados y retenidos obtenidos tras someter a la planta a un proceso de digestibilidad in vitro. Conclusión: la coinoculación con E. meliloti y H. maura podría mejorar el cultivo de la alfalfa bajo condiciones específicas de salinidad, aumentando su composición nutricional y funcional, pudiendo considerarse en la formulación de suplementos nutricionales para el consumo humano.

  20. Herbarium of the University of Malaga (Spain): Vascular Plants Collection

    PubMed Central

    García-Sánchez, José; Cabezudo, Baltasar

    2013-01-01

    la colección principal del herbario. La base de datos MGC-Cormof tiene como objetivo la digitalización y publicación de los datos asociados con los más de 76.000 ejemplares depositados en la colección, de los cuales el 97,2% de las muestras se encuentran identificadas a nivel de especie. Desde 2011, los Servicios Centrales de Investigación (SCAI) de la Universidad de Málaga son responsables de mantener el herbario y sus respectivas bases de datos. Esta colección está en continuo crecimiento, con una incorporación anual de unos 1.500 ejemplares. Casi el 96% de la colección está digitalizada, a través del programa Herbar v3.7.1 (F. Pando et al. 1996–2011) por lo que más de 73.000 especímenes son accesibles a través de la red de GBIF (http://data.gbif.org/datasets/resource/8105/). Actualmente, la colección MGC-Cormof está constituida por 247 familias y 8.110 taxones, distribuidos en angiospermas (93,97%), helechos y plantas afines (4,89%) y gimnospermas (1,14%). Las familias y géneros mejor representados en la colección son Compositae, Leguminosae, Gramineae, Labiatae, Caryophyllaceae, Teucrium, Silene, Asplenium, Linaria y Quercus. La mayoría de los especímenes provienen de la región del Mediterráneo Occidental, fundamentalmente del sur de España (Andalucía: 82% de las muestras) y del norte de Marruecos (2,17%). Aproximadamente, el 63% de las muestras se encuentran georreferenciadas. La identificación de los ejemplares de la colección ha sido realizada por personal del departamento de biología vegetal de la Universidad de Málaga y además un 40% de los ejemplares ha sido revisado por especialistas. La base de datos MGC-Cormof ha sido revisada mediante la herramienta DarwinTest v3.2 (Ortega-Maqueda and Pando 2008) antes de ser publicada en GBIF. Los datos incluidos en esta base de datos son importantes para trabajos de conservación, taxonomía, flora, cartografía, fenología, palinología, entre otros. PMID:24194668

  1. Herbarium of the university of malaga (Spain): vascular plants collection.

    PubMed

    García-Sánchez, José; Cabezudo, Baltasar

    2013-01-01

    ón principal del herbario. La base de datos MGC-Cormof tiene como objetivo la digitalización y publicación de los datos asociados con los más de 76.000 ejemplares depositados en la colección, de los cuales el 97,2% de las muestras se encuentran identificadas a nivel de especie. Desde 2011, los Servicios Centrales de Investigación (SCAI) de la Universidad de Málaga son responsables de mantener el herbario y sus respectivas bases de datos. Esta colección está en continuo crecimiento, con una incorporación anual de unos 1.500 ejemplares. Casi el 96% de la colección está digitalizada, a través del programa Herbar v3.7.1 (F. Pando et al. 1996-2011) por lo que más de 73.000 especímenes son accesibles a través de la red de GBIF (http://data.gbif.org/datasets/resource/8105/). Actualmente, la colección MGC-Cormof está constituida por 247 familias y 8.110 taxones, distribuidos en angiospermas (93,97%), helechos y plantas afines (4,89%) y gimnospermas (1,14%). Las familias y géneros mejor representados en la colección son Compositae, Leguminosae, Gramineae, Labiatae, Caryophyllaceae, Teucrium, Silene, Asplenium, Linaria y Quercus. La mayoría de los especímenes provienen de la región del Mediterráneo Occidental, fundamentalmente del sur de España (Andalucía: 82% de las muestras) y del norte de Marruecos (2,17%). Aproximadamente, el 63% de las muestras se encuentran georreferenciadas. La identificación de los ejemplares de la colección ha sido realizada por personal del departamento de biología vegetal de la Universidad de Málaga y además un 40% de los ejemplares ha sido revisado por especialistas. La base de datos MGC-Cormof ha sido revisada mediante la herramienta DarwinTest v3.2 (Ortega-Maqueda and Pando 2008) antes de ser publicada en GBIF. Los datos incluidos en esta base de datos son importantes para trabajos de conservación, taxonomía, flora, cartografía, fenología, palinología, entre otros.

  2. Three-dimensional mathematical model to simulate groundwater flow in the lower Palar River basin, southern India

    NASA Astrophysics Data System (ADS)

    Senthilkumar, M.; Elango, L.

    principales en el río, además de numerosos pozos distribuidos por la zona. El modelo simula el flujo de las aguas subterráneas en una superficie de 392 km2 por medio de 70 filas, 40 columnas y 2 capas. El modelo ha sido empleado en condiciones transitorias, por medio de la aproximación en diferencias finitas de las ecuaciones diferenciales parciales en tres dimensiones del flujo en el acuífero durante el período 1991-2001. Se ha calibrado el modelo en condiciones permanentes y transitorias. El ajuste entre los niveles calculados y medidos es razonable. A partir de los resultados de la modelación, se ha obtenido que el sistema acuífero es estable con la tasa de bombeo utilizada, exceptuando unos pocos emplazamientos a lo largo de la costa, donde se ha dado lugar a fenómenos de intrusión marina en una distancia de 50-100 m. El modelo transitorio ha sido ejecutado hasta el año 2010 para predecir el flujo dinámico bajo diversos escenarios de sobreexplotación y de reducción de la recarga. Se produce una disminución en los niveles piezométricos de 0.6 a 0.8 m en la zona oriental, donde el sistema acuífero está sometido a una extracción adicional de 2 millones de galones por día en la estación principal de bombeo. Incluso con las extracciones actuales, los niveles piezométricos se sitúan bajo el nivel del mar durante las épocas secas. El modelo predice el comportamiento de este sistema acuífero bajo varias condiciones de presión hidrológica.

  3. Regulation of fixLJ by Hfq Controls Symbiotically Important Genes in Sinorhizobium meliloti.

    PubMed

    Gao, Mengsheng; Nguyen, Hahn; Salas González, Isai; Teplitski, Max

    2016-11-01

    The RNA-binding chaperone Hfq plays critical roles in the establishment and functionality of the symbiosis between Sinorhizobium meliloti and its legume hosts. A mutation in hfq reduces symbiotic efficiency resulting in a Fix(-) phenotype, characterized by the inability of the bacterium to fix nitrogen. At least in part, this is due to the ability of Hfq to regulate the fixLJ operon, which encodes a sensor kinase-response regulator pair that controls expression of the nitrogenase genes. The ability of Hfq to bind fixLJ in vitro and in planta was demonstrated with gel shift and coimmunoprecipitation experiments. Two (ARN)2 motifs in the fixLJ message were the likely sites through which Hfq exerted its posttranscriptional control. Consistent with the regulatory effects of Hfq, downstream genes controlled by FixLJ (such as nifK, noeB) were also subject to Hfq regulation in planta.

  4. Protein fragment bimolecular fluorescence complementation analyses for the in vivo study of protein-protein interactions and cellular protein complex localizations

    PubMed Central

    Waadt, Rainer; Schlücking, Kathrin; Schroeder, Julian I.; Kudla, Jörg

    2014-01-01

    Summary The analyses of protein-protein interactions is crucial for understanding cellular processes including signal transduction, protein trafficking and movement. Protein fragment complementation assays are based on the reconstitution of protein function when non-active protein fragments are brought together by interacting proteins that were genetically fused to these protein fragments. Bimolecular fluorescence complementation (BiFC) relies on the reconstitution of fluorescent proteins and enables both the analysis of protein-protein interactions and the visualization of protein complex formations in vivo. Transient expression of proteins is a convenient approach to study protein functions in planta or in other organisms, and minimizes the need for time-consuming generation of stably expressing transgenic organisms. Here we describe protocols for BiFC analyses in Nicotiana benthamiana and Arabidopsis thaliana leaves transiently transformed by Agrobacterium infiltration. Further we discuss different BiFC applications and provide examples for proper BiFC analyses in planta. PMID:24057390

  5. Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis.

    PubMed

    Mourad, George S; Tippmann-Crosby, Julie; Hunt, Kevin A; Gicheru, Yvonne; Bade, Kaely; Mansfield, Tyler A; Schultes, Neil P

    2012-05-07

    Locus At5g03555 encodes a nucleobase cation symporter 1 (AtNCS1) in the Arabidopsis genome. Arabidopsis insertion mutants, AtNcs1-1 and AtNcs1-3, were used for in planta toxic nucleobase analog growth studies and radio-labeled nucleobase uptake assays to characterize solute transport specificities. These results correlate with similar growth and uptake studies of AtNCS1 expressed in Saccharomyces cerevisiae. Both in planta and heterologous expression studies in yeast revealed a unique solute transport profile for AtNCS1 in moving adenine, guanine and uracil. This is in stark contrast to the canonical transport profiles determined for the well-characterized S. cerevisiae NCS1 proteins FUR4 (uracil transport) or FCY2 (adenine, guanine, and cytosine transport).

  6. Cyanophora paradoxa genome elucidates origin of photosynthesis in algae and plants.

    PubMed

    Price, Dana C; Chan, Cheong Xin; Yoon, Hwan Su; Yang, Eun Chan; Qiu, Huan; Weber, Andreas P M; Schwacke, Rainer; Gross, Jeferson; Blouin, Nicolas A; Lane, Chris; Reyes-Prieto, Adrián; Durnford, Dion G; Neilson, Jonathan A D; Lang, B Franz; Burger, Gertraud; Steiner, Jürgen M; Löffelhardt, Wolfgang; Meuser, Jonathan E; Posewitz, Matthew C; Ball, Steven; Arias, Maria Cecilia; Henrissat, Bernard; Coutinho, Pedro M; Rensing, Stefan A; Symeonidi, Aikaterini; Doddapaneni, Harshavardhan; Green, Beverley R; Rajah, Veeran D; Boore, Jeffrey; Bhattacharya, Debashish

    2012-02-17

    The primary endosymbiotic origin of the plastid in eukaryotes more than 1 billion years ago led to the evolution of algae and plants. We analyzed draft genome and transcriptome data from the basally diverging alga Cyanophora paradoxa and provide evidence for a single origin of the primary plastid in the eukaryote supergroup Plantae. C. paradoxa retains ancestral features of starch biosynthesis, fermentation, and plastid protein translocation common to plants and algae but lacks typical eukaryotic light-harvesting complex proteins. Traces of an ancient link to parasites such as Chlamydiae were found in the genomes of C. paradoxa and other Plantae. Apparently, Chlamydia-like bacteria donated genes that allow export of photosynthate from the plastid and its polymerization into storage polysaccharide in the cytosol.

  7. GenoCAD Plant Grammar to Design Plant Expression Vectors for Promoter Analysis.

    PubMed

    Coll, Anna; Wilson, Mandy L; Gruden, Kristina; Peccoud, Jean

    2016-01-01

    With the rapid advances in prediction tools for discovery of new promoters and their cis-elements, there is a need to improve plant expression methodologies in order to facilitate a high-throughput functional validation of these promoters in planta. The promoter-reporter analysis is an indispensible approach for characterization of plant promoters. It requires the design of complex plant expression vectors, which can be challenging. Here, we describe the use of a plant grammar implemented in GenoCAD that will allow the users to quickly design constructs for promoter analysis experiments but also for other in planta functional studies. The GenoCAD plant grammar includes a library of plant biological parts organized in structural categories to facilitate their use and management and a set of rules that guides the process of assembling these biological parts into large constructs.

  8. Establishment of Lipolexis oregmae (Hymenoptera: Aphidiidae) in a classical biological control program directed against the brown citrus aphid (Homoptera: Aphididae) in Florida

    SciTech Connect

    Persad, A.B.; Hoy, M.A.; Ru Nguyen

    2007-03-15

    Guam, evaluado en cuarentena, criado en masa y liberado en huertos de citricos en un programa de control biologico clasico dirigido contra el afido pardo de citricos, Toxoptera citricida Kirkaldy. Se hicieron liberaciones de 20,200, 12,100, y 1,260 adultos de L. oregmae a traves de la Florida durante los anos de 2000, 2001, y 2002, respectivamente. Para determinar si L. oregmae ha logrado en establecer, se realizaron sondeos a traves del estado empezando en el verano del 2001 y continuando hasta el final del verano del 2003. El parasitismo durante 2001 y 2002 fue evaluado con el mantenimiento de individuos del afido pardo de los citricos en el laboratorio hasta que los adultos emergieron. Lipolexis oregmae fue encontrado en 10 sitios en 7 condados y con tasas de parasitismo en 4 sitios en 3 condados entre 0.7 a 3.3% en el 2001 y 2002, respectivamente. Las pruebas del laboratorio indicaron que las tasas altas de mortalidad fueron posibles si los afidos con parasitos recolectados en el campo fueron mantenidos en bolsas plasticas, entonces un ensayo molecular fue usado con lo que permitio la deteccion de inmaduros de L. oregmae dentro de los hospederos de afidos inmediatamente despues de la recoleccion. El ensayo molecular fue usado en el 2003 con individuos del afido pardo de los citricos y con otras especies de afidos recolectados sobre citricos, malezas y hortalizas cerca de los sitios donde los parasitoides fueron liberados anteriormente; inmaduros de L. oregmae fueron detectados en individuos del afido negro de los citricos, el afido del caupi, el afido spirea y el afido del melon, ademas del afido pardo de los citricos en 4 de los 8 condados muestreados, con la tasa del parasitismo entre 2.0 a12.9%, indicando que L. oregmae estaba estabecido y ampliamente distribuido. Las muestras tomadas en el Condado de Polk durante octobre del 2005 indicaron que L. oregmae ha persistido. La capacidad de L. oregmae para parasitar otras especies de afidos sobre citricos y otros

  9. Biosynthesis of the antimetabolite 6-thioguanine in Erwinia amylovora plays a key role in fire blight pathogenesis.

    PubMed

    Coyne, Sébastien; Chizzali, Cornelia; Khalil, Mohammed N A; Litomska, Agnieszka; Richter, Klaus; Beerhues, Ludger; Hertweck, Christian

    2013-09-27

    Sulfur for fire: The molecular basis for the biosynthesis of the antimetabolite 6-thioguanine (6TG) was unveiled in Erwinia amylovora, the causative agent of fire blight. Bioinformatics, heterologous pathway reconstitution in E. coli, and mutational analyses indicate that the protein YcfA mediates guanine thionation in analogy to 2-thiouridylase. Assays in planta and in cell cultures reveal for the first time a crucial role of 6TG in fire blight pathogenesis.

  10. Enhancement of Drain-Down Capabilities of Submarine Antennae

    DTIC Science & Technology

    2007-07-01

    Measurement Contact angles were measured with a Rame-Hart Model 100 goniometer. This is a manual instrument equipped with a 20” rail, 3 stage axis...placed at three positions on the coupons: centre left, centre, and centre right. The advancing and receding contact angles were recorded manually at...Neinhuis C. (1997) Purity of the sacred lotus, or escape from contamination in biological surfaces. Planta , 202, 1-8 [2] Gao X.F. & Jiang L. (2004

  11. A Single Amino Acid Change in Turnip Crinkle Virus Movement Protein p8 Affects RNA Binding and Virulence on Arabidopsis thaliana

    PubMed Central

    Wobbe, Kristin K.; Akgoz, Muslum; Dempsey, D’Maris Amick; Klessig, Daniel F.

    1998-01-01

    Comparison of the symptoms caused by turnip crinkle virus strain M (TCV-M) and TCV-B infection of a resistant Arabidopsis thaliana line termed Di-17 demonstrates that TCV-B has a greater ability to spread in planta. This ability is due to a single amino acid change in the viral movement protein p8 and inversely correlates with p8 RNA binding affinity. PMID:9621099

  12. Human Performance and Biosystems

    DTIC Science & Technology

    2013-03-08

    M., Grossman, A.R., and Posewitz. M.C. (2012) Genetic disruption of both Chlamydomonas reinhardtii [ FeFe ]-hydrogenases: insight into the role of...structure of HydF scaffold protein provides insights into [ FeFe ]-hydrogenase maturation. Journal of Biological Chemistry 286, 43944-43950. 8...variabilis NC64A [ FeFe ]-hydrogenase. Planta 234, 829-843. CSM Publications citing AFOSR funding during the last year DISTRIBUTION A: Approved for

  13. Europe/Latin America Report, Science and Technology.

    DTIC Science & Technology

    2007-11-02

    modern, export oriented economy; —development of human life potentials ( health , living conditions , working conditions , employment possibilities...of satellites, parabolic flights with experimental rockets for scientific and industrial tests under weightless conditions , and even the...rather small slice is taken from a place in the plant~a carrot, for example-and this slice is transferred under sterile conditions to a so-called solid

  14. Houma Navigation Canal Deepening Project Terrebonne Parish, Louisiana: Cultural Resources Literature Search, Records Review and Research Design

    DTIC Science & Technology

    2005-03-01

    sections along bayous Chene, Black, Boeuf, research throughout the entire Atchafalaya Basin and and the Avoca Island Cutoff. Forty-three sites were...journals of the expedition Cathcart noted that the western portion, at least, of provide additional information on settlements in the "Cowpen" ( Avoca ...inhabitants on Avoca Island, although section of Bayou Boeuf running roughly north-south a good bit of detail is provided on Bryant’s Planta- between

  15. Area Handbook Series. Cote D’Lvoire; A Country Study

    DTIC Science & Technology

    1988-11-01

    for antibodies to human immunodeficiency virus (HIV), which causes acquired immunodeficiency syndrome (AIDS). By the end of that year, it had reports...level to be defended. Coffee Cte d’Ivoire ranked third in world coffee production after Brazil and Colombia . Introduced as a cash crop during the...substantially higher salaries, Through- out the country, there were French mechanics, foremen, planta - tion’owners, storekeepers, clerical workers, and

  16. Cultural Resources Assessment of the Faulkner Lake Revetment, East Baton Rouge Parish, Louisiana.

    DTIC Science & Technology

    1987-05-06

    Bartram Adm. David Farragut Loess Mobile Ridge Baton Rouge Faulkner Lake Lunette Mount Pleasant Planta- Bayou Sara Fossil flora Charles Lyell tion...travellers and naturalists, including William Bartram and Charles Lyell (Bartram Trail Conference 1979; Lyell 1849). Geomorphic and Ecological...Holocene Vegetation Record from the Mississippi River Valley, Southeastern Missouri. Quaternary Research 8:307-323. Lyell , Charles 1849 A Second Visit to the

  17. The C-terminal extension of PrhG impairs its activation of hrp expression and virulence in Ralstonia solanacearum.

    PubMed

    Zhang, Yong; Luo, Feng; Hikichi, Yasufumi; Kiba, Akinori; Yasuo, Igarashi; Ohnishi, Kouhei

    2015-04-01

    Ralstonia solanacearum is the second most destructive bacterial plant pathogens worldwide and HrpG is the master regulator of its pathogenicity. PrhG is a close paralogue of HrpG and both belong to OmpR/PhoB family of two-component response regulators. Despite a high similarity (72% global identity and 96% similarity in helix-loop-helix domain), they display distinct roles in pathogenicity. HrpG is necessary for the bacterial growth in planta and pathogenicity, while PrhG is dispensable for bacterial growth in planta and contributes little to pathogenicity. The main difference between HrpG and PrhG is the 50-amino-acid-long C-terminal extension in PrhG (amino-acid residues 230-283), which is absent in HrpG. When this extension is deleted, truncated PrhGs (under the control of its native promoter) allowed complete recovery of bacterial growth in planta and wild-type virulence of hrpG mutant. This novel finding demonstrates that the extension region in PrhG is responsible for the functional difference between HrpG and PrhG, which may block the binding of PrhG to target promoters and result in impaired activation of hrp expression by PrhG and reduced virulence of R. solanacearum.

  18. Paracercomonas kinetid ultrastructure, origins of the body plan of Cercomonadida, and cytoskeleton evolution in Cercozoa.

    PubMed

    Cavalier-Smith, Thomas; Karpov, Sergey A

    2012-01-01

    Serial section reconstruction shows that kinetid ultrastructure in two genetically divergent Paracercomonas (P. virgaria, P. metabolica) is basically similar, differing somewhat from clade A cercomonads. Paracercomonas (Paracercomonadidae fam. n.) have a posterior root (dp1) attached to the posterior centriole, unlike Cercomonadidae (here revised to include only Eocercomonas, Cercomonas, Filomonas gen. n., and Neocercomonas), which belong in clade A (new suborder Cercomonadina) with Cavernomonas (Cavernomonadidae fam. n.). Whether dp1 is serially homologous to anterior root da is unclear. The common ancestor of Cercomonadida probably had five microtubular roots, two fibrillar microtubule-nucleating centres generating microtubular cones, and striated connectors between obtusely angled centrioles. Our new data leave the question of holophyly versus polyphyly of Cercomonadida unresolved, but clarify cercozoan root diversity and homologies. Ventral root vp1 is throughout Cercozoa; vp2 might be restricted to the new superclass Ventrifilosa plus Sarcomonadea. Though cercozoan microtubular arrangements differ substantially from others within the kingdom Chromista, the microtubular root numbering system used for other chromists and Plantae is applicable to them; in doing this we found that the single anterior root of excavates (probably ancestral to Chromista, Plantae and unikonts) and Euglenozoa corresponds with R3 (not R4 as previously thought) of corticate eukaryotes (Chromista plus Plantae).

  19. Expression of sea anemone equistatin in potato. Effects of plant proteases on heterologous protein production.

    PubMed

    Outchkourov, Nikolay S; Rogelj, Boris; Strukelj, Borut; Jongsma, Maarten A

    2003-09-01

    Plants are increasingly used as production platforms of various heterologous proteins, but rapid protein turnover can seriously limit the steady-state expression level. Little is known about specific plant proteases involved in this process. In an attempt to obtain potato (Solanum tuberosum cv Desirée) plants resistant to Colorado potato beetle (Leptinotarsa decemlineata Say) larvae, the protease inhibitor equistatin was expressed under the control of strong, light-inducible and constitutive promoters and was targeted to the secretory pathway with and without endoplasmic reticulum retention signal. All constructs yielded similar stepwise protein degradation patterns, which considerably reduced the amount of active inhibitor in planta and resulted in insufficient levels for resistance against Colorado potato beetle larvae. Affinity purification of the degradation products and N-terminal sequencing allowed the identification of the amino acid P(1)-positions (asparagine [Asn]-13, lysine-56, Asn-82, and arginine-151) that were cleaved in planta. The proteases involved in the equistatin degradation were characterized with synthetic substrates and inhibitors. Kininogen domain 3 completely inhibited equistatin degradation in vitro. The results indicate that arginine/lysine-specific and legumain-type Asn-specific cysteine proteases seriously impede the functional accumulation of recombinant equistatin in planta. General strategies to improve the resistance to proteases of heterologous proteins in plants are proposed.

  20. Control of endophytic Frankia sporulation by Alnus nodule metabolites.

    PubMed

    Hay De-Bettignies, Anne-Emmanuelle; Boubakri, Hasna; Buonomo, Antoine; Rey, Marjolaine; Meiffren, Guillaume; Cotin-Galvan, Laetitia; Comte, Gilles; Herrera-Belaroussi, Aude

    2017-01-10

    A unique case of microbial symbiont capable of dormancy within its living host cells has been reported in actinorhizal symbioses: some Frankia strains, named Sp+, are able to sporulate inside plant cells, contrarily to Sp- strains. The presence of metabolically slowed down bacterial structures in host cells alters our understanding of symbiosis based on reciprocal benefits between both partners, and its impact on the symbiotic processes remains unknown. The present work reports a metabolomic study of Sp+ and Sp- nodules (from Alnus glutinosa), in order to highlight variabilities associated with in-planta sporulation. A total of 21 amino acids (AA), 44 sugars and organic acids (SOA), and 213 secondary metabolites (M) were detected using UV and mass spectrometric-based profiling. Little change was observed in primary metabolites, suggesting that in-planta sporulation would not strongly affect the primary functionalities of the symbiosis. One secondary metabolite (M27) was detected only in Sp+ nodules. It was identified as Gentisic acid 5-O-β-D-xylopyranoside, previously reported as involved in plant defenses against microbial pathogens. This metabolite significantly increased Frankia in-vitro sporulation, unlike another metabolite significantly more abundant in Sp- nodules (M168 = (5R)-1,7-bis-(3,4-) dihydroxyphenyl)-heptane-5-O-β-D-glucopyranoside). All these results suggest that the plant could play an important role in Frankia ability to sporulate in-planta, and allow us to discuss a possible sanction emitted by the host against less cooperative Sp+ symbionts.

  1. Identification of Botrytis cinerea genes up-regulated during infection and controlled by the Galpha subunit BCG1 using suppression subtractive hybridization (SSH).

    PubMed

    Schulze Gronover, Christian; Schorn, Corinna; Tudzynski, Bettina

    2004-05-01

    The Galpha subunit BCG1 plays an important role during the infection of host plants by Botrytis cinerea. Delta bcg1 mutants are able to conidiate, penetrate host leaves, and produce small primary lesions. However, in contrast to the wild type, the mutants completely stop invasion of plant tissue at this stage; secondary lesions have never been observed. Suppression subtractive hybridization (SSH) was used to identify fungal genes whose expression on the host plant is specifically affected in bcg1 mutants. Among the 22 differentially expressed genes, we found those which were predicted to encode proteases, enzymes involved in secondary metabolism, and others encoding cell wall-degrading enzymes. All these genes are highly expressed during infection in the wild type but not in the mutant. However, the genes are expressed in both the wild type and the mutant under certain conditions in vitro. Most of the BCG1-controlled genes are still expressed in adenylate cyclase (bac) mutants in planta, suggesting that BCG1 is involved in at least one additional signaling cascade in addition to the cAMP-depending pathway. In a second SSH approach, 1,500 clones were screened for those that are specifically induced by the wild type during the infection of bean leaves. Of the 22 BCG1-controlled genes, 11 also were found in the in planta SSH library. Therefore, SSH technology can be successfully applied to identify target genes of signaling pathways and differentially expressed genes in planta.

  2. Identification of loci of Pseudomonas syringae pv. actinidiae involved in lipolytic activity and their role in colonization of kiwifruit leaves.

    PubMed

    Kumar Patel, Hitendra; Ferrante, Patrizia; Xianfa, Meng; Javvadi, Sree Gowrinadh; Subramoni, Sujatha; Scortichini, Marco; Venturi, Vittorio

    2017-01-23

    Bacterial canker disease caused by Pseudomonas syringae pv. actinidiae (Psa), an emerging pathogen of kiwifruit plants, has recently brought about major economic losses worldwide. Genetic studies on virulence functions of Psa have not yet been reported and there is little experimental data regarding bacterial genes involved in pathogenesis. In this study, we performed a genetic screen in order to identify transposon mutants altered in the lipolytic activity as it is known that mechanisms of regulation, production and secretion of enzymes often play crucial roles in virulence of plant pathogens. We aimed to identify the set of secretion and global regulatory loci that control lipolytic activity and also play important roles in in planta fitness. Our screen for altered lipolytic activity phenotype identified a total of 58 Tn5 transposon mutants. Mapping all these Tn5 mutants revealed that the transposons were inserted in genes that play roles in cell division, chemotaxis, metabolism, movement, recombination, regulation, signal transduction, and transport as well as a few unknown functions. Several of these identified Psa Tn5 mutants, notably the functions affected in phosphomannomutase AlgC, lipid A biosynthesis acyltransferase, glutamate--cysteine ligase and the type IV pilus protein PilI, were also found affected in in planta survival and/or growth in kiwifruit plants. The results of the genetic screen and identification of novel loci involved in in planta fitness of Psa are presented and discussed.

  3. SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl− accumulation and salt tolerance in Arabidopsis thaliana

    PubMed Central

    Qiu, Jiaen; Henderson, Sam W; Tester, Mark; Roy, Stuart J; Gilliham, Mathew

    2016-01-01

    Salinity tolerance is correlated with shoot chloride (Cl–) exclusion in multiple crops, but the molecular mechanisms of long-distance Cl– transport are poorly defined. Here, we characterize the in planta role of AtSLAH1 (a homologue of the slow type anion channel-associated 1 (SLAC1)). This protein, localized to the plasma membrane of root stelar cells, has its expression reduced by salt or ABA, which are key predictions for a protein involved with loading Cl– into the root xylem. Artificial microRNA knockdown mutants of AtSLAH1 had significantly reduced shoot Cl− accumulation when grown under low Cl–, whereas shoot Cl– increased and the shoot nitrate/chloride ratio decreased following AtSLAH1 constitutive or stelar-specific overexpression when grown in high Cl–. In both sets of overexpression lines a significant reduction in shoot biomass over the null segregants was observed under high Cl– supply, but not low Cl– supply. Further in planta data showed AtSLAH3 overexpression increased the shoot nitrate/chloride ratio, consistent with AtSLAH3 favouring nitrate transport. Heterologous expression of AtSLAH1 in Xenopus laevis oocytes led to no detectible transport, suggesting the need for post-translational modifications for AtSLAH1 to be active. Our in planta data are consistent with AtSLAH1 having a role in controlling root-to-shoot Cl– transport. PMID:27340232

  4. Phosphorylation of HopQ1, a Type III Effector from Pseudomonas syringae, Creates a Binding Site for Host 14-3-3 Proteins1[C][W][OA

    PubMed Central

    Giska, Fabian; Lichocka, Małgorzata; Piechocki, Marcin; Dadlez, Michał; Schmelzer, Elmon; Hennig, Jacek; Krzymowska, Magdalena

    2013-01-01

    HopQ1 (for Hrp outer protein Q), a type III effector secreted by Pseudomonas syringae pv phaseolicola, is widely conserved among diverse genera of plant bacteria. It promotes the development of halo blight in common bean (Phaseolus vulgaris). However, when this same effector is injected into Nicotiana benthamiana cells, it is recognized by the immune system and prevents infection. Although the ability to synthesize HopQ1 determines host specificity, the role it plays inside plant cells remains unexplored. Following transient expression in planta, HopQ1 was shown to copurify with host 14-3-3 proteins. The physical interaction between HopQ1 and 14-3-3a was confirmed in planta using the fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy technique. Moreover, mass spectrometric analyses detected specific phosphorylation of the canonical 14-3-3 binding site (RSXpSXP, where pS denotes phosphoserine) located in the amino-terminal region of HopQ1. Amino acid substitution within this motif abrogated the association and led to altered subcellular localization of HopQ1. In addition, the mutated HopQ1 protein showed reduced stability in planta. These data suggest that the association between host 14-3-3 proteins and HopQ1 is important for modulating the properties of this bacterial effector. PMID:23396834

  5. Plant and animal glycolate oxidases have a common eukaryotic ancestor and convergently duplicated to evolve long-chain 2-hydroxy acid oxidases.

    PubMed

    Esser, Christian; Kuhn, Anke; Groth, Georg; Lercher, Martin J; Maurino, Veronica G

    2014-05-01

    Glycolate oxidase (GOX) is a crucial enzyme of plant photorespiration. The encoding gene is thought to have originated from endosymbiotic gene transfer between the eukaryotic host and the cyanobacterial endosymbiont at the base of plantae. However, animals also possess GOX activities. Plant and animal GOX belong to the gene family of (L)-2-hydroxyacid-oxidases ((L)-2-HAOX). We find that all (L)-2-HAOX proteins in animals and archaeplastida go back to one ancestral eukaryotic sequence; the sole exceptions are green algae of the chlorophyta lineage. Chlorophyta replaced the ancestral eukaryotic (L)-2-HAOX with a bacterial ortholog, a lactate oxidase that may have been obtained through the primary endosymbiosis at the base of plantae; independent losses of this gene may explain its absence in other algal lineages (glaucophyta, rhodophyta, and charophyta). We also show that in addition to GOX, plants possess (L)-2-HAOX proteins with different specificities for medium- and long-chain hydroxyacids (lHAOX), likely involved in fatty acid and protein catabolism. Vertebrates possess lHAOX proteins acting on similar substrates as plant lHAOX; however, the existence of GOX and lHAOX subfamilies in both plants and animals is not due to shared ancestry but is the result of convergent evolution in the two most complex eukaryotic lineages. On the basis of targeting sequences and predicted substrate specificities, we conclude that the biological role of plantae (L)-2-HAOX in photorespiration evolved by co-opting an existing peroxisomal protein.

  6. Knowledge and Beliefs of Breast Cancer Among Elderly Puerto Rican Women.

    DTIC Science & Technology

    1995-10-01

    momento d6 por terminada la prirnera entrevista. Acuerde .la ifecha de la pr6xirna cita para den tro de dos a tres semanas. Debe tra tar de hacerle...34 refleja el grado de correspondencia entre las contestaciones a preguntas hechas a las mismas personas en momentos diferentes del tiempo (entre 2 a 4...entrevistadores ("Inter-observer reliability") y al grado de acuerdo entre, las observaciones del mismo entrevistador en diferentes momentos del tiempo

  7. [A study using positron emission tomography of a case of vascular dementia due to left thalamic haematoma, an example of the diaschisis phenomenon].

    PubMed

    Blanco-Martin, E; Del Mazo-Sanchez, S; Molano-Salazar, A; Bereincua-Gandarias, R; Llorens-Abando, V; Fernandez-Martinez, M

    2016-05-01

    Introduccion. Las lesiones vasculares talamicas que se comportan como ictus estrategicos pueden causar amnesia, disfunciones ejecutivas o disfasia, asi como sintomas comportamentales o psicologicos, y causar una demencia vascular. Caso clinico. Mujer de 58 años, hipertensa y dislipidemica, que, tras una hemorragia talamica izquierda que evoluciono radiologicamente de manera favorable, presento un sindrome amnesico grave y otras alteraciones sutiles en la orientacion y el lenguaje, dificultades en el manejo del dinero y sintomas depresivos que precisaron tratamiento ansiolitico y antidepresivo, todo lo cual fue causa de limitaciones para el normal desempeño de su trabajo. Seguida en la consulta de neurologia, se le practico una tomografia por emision de positrones/tomografia axial computarizada con 18F-2-fluoro-2-desoxi-D-glucosa, donde se aprecio un hipometabolismo en el talamo izquierdo y, ademas, en la region frontal inferior ipsilateral, que se explicaria mediante el fenomeno de diasquisis. Conclusiones. El fenomeno de diasquisis es un hallazgo de neuroimagen y fisiopatologico por el cual los ictus talamicos o de los ganglios basales causan hipoperfusion/hipometabolismo en la corteza ipsilateral o contralateral, y que puede explicar sintomas a distancia corticales. El presente caso evidencia la presencia de conexiones talamocorticales, lo cual ayuda a comprender los circuitos de la memoria y a explicar la asociacion en el de otros sintomas corticales, como la disfasia o las alteraciones ejecutivas.

  8. Dispersión de velocidades en cúmulos de galaxias

    NASA Astrophysics Data System (ADS)

    Muriel, H.; García Lambas, D.; Quintana, H.; Infante, L.

    Se ha estudiado la dinámica de galaxias en cúmulos en base a una muestra de aproximadamente 4000 galaxias con estimas de velocidad radial pertenecientes a 40 cúmulos. Se desarrolló un algoritmo para identificar sub-estructuras del tipo ``grupos de galaxias". Una vez eliminada este tipo de sub-estructura se computaron los perfiles radiales de dispersión de velocidades (VDP) para los 40 cúmulos de galaxias. Dada las características de la muestra, fue posible estimar el VDP hasta grandes distancias del centro del cúmulo llegando en algunos casos hasta 7 Mpc-1. Los resultados indican que el 65% de los cúmulos presentan un VDP plano a grandes radios consistente con el modelo isotermo. Estos resultados son discutidos en el marco de las recientes controversias suscitadas respecto de la dinámica de los cúmulos vía los perfiles de temperaturas de cúmulos derivados de la emisión en rayos-X.

  9. Social identity, passion and well-being in university students, the mediating effect of passion.

    PubMed

    Bernabé, Miguel; Lisbona, Ana; Palací, Francisco José; Martín-Aragón, Maite

    2014-11-14

    Research on positive emotions associated with the performance of an activity, such as work or study, has increased exponentially in recent years. Passion is understood as an attitude and intense emotion in the performance of an activity, and it has shown both positive and negative consequences for well-being. A link between social identity and positive emotions through social category membership has been described. The aim of this work is to study the relationship between social identity, the dimensions of passion and the positive impact on university responses. A quasi-experimental design was used on a sample of 266 university students from different Spanish universities (Universidad Nacional de Educación a Distancia, Universidad Miguel Hernández and Universidad de Alicante). Descriptive analyzes were performed on the study's variables using SPSS 18. Structural equation modeling was carried out with AMOS 18 and the mediational analysis with MODMED macro developed by Preacher, Rucker, and Hayes (2007). The results show that the identity of the studies had an indirect effect on positive responses mediated by passion for the studies (RMSEA = .07; CFI = .97; NFI = .96; TLI = .92). It is observed that the harmonious and obsessive dimensions of passion differ in the mediating effect on happiness and satisfaction with studies. Practical and theoretical implications for well-being are discussed.

  10. Teleconferences and Audiovisual Materials in Earth Science Education

    NASA Astrophysics Data System (ADS)

    Cortina, L. M.

    2007-05-01

    Unidad de Educacion Continua y a Distancia, Universidad Nacional Autonoma de Mexico, Coyoaca 04510 Mexico, MEXICO As stated in the special session description, 21st century undergraduate education has access to resources/experiences that go beyond university classrooms. However in some cases, resources may go largely unused and a number of factors may be cited such as logistic problems, restricted internet and telecommunication service access, miss-information, etc. We present and comment on our efforts and experiences at the National University of Mexico in a new unit dedicated to teleconferences and audio-visual materials. The unit forms part of the geosciences institutes, located in the central UNAM campus and campuses in other States. The use of teleconference in formal graduate and undergraduate education allows teachers and lecturers to distribute course material as in classrooms. Course by teleconference requires learning and student and teacher effort without physical contact, but they have access to multimedia available to support their exhibition. Well selected multimedia material allows the students to identify and recognize digital information to aid understanding natural phenomena integral to Earth Sciences. Cooperation with international partnerships providing access to new materials and experiences and to field practices will greatly add to our efforts. We will present specific examples of the experiences that we have at the Earth Sciences Postgraduate Program of UNAM with the use of technology in the education in geosciences.

  11. Foco Nasmyth para el telescopio 2,15mts. de CASLEO

    NASA Astrophysics Data System (ADS)

    Casagrande, A. R.

    En principio, este proyecto intenta lograr el mayor aprovechamiento posible del instrumental que se dispone, buscando la manera de optimizar y hacer más eficiente el servicio que brinda el CASLEO a la comunidad astronómica. El mismo consiste en utilizar dispositivos ya existentes en el telescopio, y darle una utilidad. Tal es el caso del camino óptico destinado al foco Coude. Si tenemos en cuenta que disponemos de un tercer espejo Coude, con todos sus mecanismos automatizados, (actualmente sin uso), una distancia apropiada del plano focal, el espacio y el lugar físico necesario para instalar un periférico, es posible la habilitación de un foco Nasmyth en el telescopio 2,15mts. El hecho de contar con este nuevo foco, redundará en importantes beneficios. En primer lugar, posibilitará la observación, casi simultánea, con dos instrumentos. Otro aspecto a tener en cuenta, es que disminuirá el frecuente cambio del instrumental periférico, motivo este que degrada su ideal puesta a punto. Por último, también de interés, es de destacar su escaso costo de ejecución.

  12. Telescopio Solar en Hα (HASTA)

    NASA Astrophysics Data System (ADS)

    Fernández Borda, R.; Francile, C.; Bagala, G.; Bauer, O.; Haerendel, G.; Rieger, E.; Rovira, M.

    El Telescopio Solar en Hα (Hα Solar Telescope for Argentina (-HASTA-) fue recientemente instalado en la Estación Astronómica de Altura Carlos Ulrrico Cesco (OAFA) en El Leoncito. Este instrumento estudiará la evolución de las fulguraciones solares desde tierra con alta resolución temporal y espacial. HASTA es un telescopio refractor de 110 mm con una distancia focal de 165 cm, un filtro Lyot sintonizable (±1Å) con un ancho de banda de 0.3 Å y una cámara CCD de alta resolución (1280×1024 pixels). Todo el conjunto es controlado en forma remota por una PC, la cual además adquiere datos del Sistema de Posicionamiento Global (GPS) y de la estación meteorológica. Diseñado para detectar fulguraciones, el instrumento trabaja en dos modos (patrullaje y fulguración) con una resolución espacial de 1.5 arcseg por pixel y una resolución temporal de 2 seg. HASTA comenzó a operar en Mayo de 1998 y, junto con otros tres instrumentos solares, que se están instalando o se instalaron, harán de El Leoncito un polo de observación solar importante en el Hemisferio Sur.

  13. Healing war wounds and perfuming exile: the use of vegetal, animal, and mineral products for perfumes, cosmetics, and skin healing among Sahrawi refugees of Western Sahara

    PubMed Central

    2012-01-01

    exilio: el uso de productos vegetales, animales y minerales con fines de perfumería, cosmética y curativos de la piel entre los refugiados saharauis del Sáhara Occidental. Antecedentes Durante la última década ha habido un creciente interés en los estudios etnobiológicos de los conocimientos y prácticas de las personas que migran. Dentro de esta tendencia, los estudiosos han prestado relativamente menor atención a las personas desplazadas y a los refugiados: a la pérdida, el mantenimiento y la adaptación de sus conocimientos etnobiológicos, y su importancia para el bienestar de los refugiados. Este estudio se centra en los cosméticos y remedios para curar problemas de la piel tradicionalmente utilizados por los refugiados saharauis en los campamentos de desplazados al sudoeste de Argelia. Métodos Los métodos de investigación que se utilizaron son: una encuesta estructurada con 37 familias de refugiados, entrevistas semi-estructuradas con 77 refugiados, 24 entrevistas retrospectivas con refugiados e informantes conocedores, y una colección de muestra de las plantas y productos citados. Resultados Se registró el uso de 55 especies vegetales, nueve especies animales, y seis productos minerales utilizados en tres principales categorías de usos: 1) Recursos contra los problemas de salud característicos del entorno desértico donde una vez vivieron los saharauis como nómadas y donde ahora viven como refugiados (por ejemplo problemas en los ojos); 2) Remedios para heridas que reflejan la historia reciente de guerra de guerrilla de los nómadas saharauis; y 3) Cosméticos y productos utilizados para el cuidado del cuerpo, decoración y perfumes (por ejemplo, atención al cabello, limpieza de dientes, uso del henna) y como aromatizantes del aire al interior de las tiendas, que son ampliamente utilizados en la vida y las prácticas sociales cotidianas de los refugiados. Conclusiones En la discusión, se analizan los cambios que se han producido en los patrones

  14. Vegetable Purée: A Pilot Study to Increase Vegetable Consumption among School Lunch Participants in US Elementary Schools

    ERIC Educational Resources Information Center

    Vale, Angela; Schumacher, Julie Raeder; Cullen, Robert W.; Gam, Hae Jin

    2014-01-01

    Purpose/Objectives: Recent US Department of Agriculture regulations increased the amount and variety of vegetables required for school lunches. Vegetables are the most wasted components of lunch while entrées are selected and consumed by the majority of children. This study examined how adding vegetable purée to an elementary school lunch entrée…

  15. Why Cold-Wet Makes One Feel Chilled: A Literature Review

    DTIC Science & Technology

    1988-06-01

    froid et mouill6. On examine aussi l’effet de la radiation solaire , l’interaction entre la peau at l’humidit6, entre la peau et la temp~rature de mgme...temperatures also were similar at low humidities. In Burton’s experiments, four out of the nine experiments at the "low" humidity had similar amounts of

  16. Environmental Effects on Materials for Space Applications.

    DTIC Science & Technology

    1983-03-01

    diamltres extlrleurs respectifs 50 et 32 mm. Lea noeuds de jonction entre barres sont entltement r~alisgs en mat~riaux composites carbone/r~sine Epoxy...schiass des figures 7 at 8. La jonction entre deux barres 0 50 eat assurfe par un gousset en croix et quatre quarts de renforts. La liaison barre 032

  17. Swimming motility, a virulence trait of Ralstonia solanacearum, is regulated by FlhDC and the plant host environment.

    PubMed

    Tans-Kersten, Julie; Brown, Darby; Allen, Caitilyn

    2004-06-01

    Swimming motility allows the bacterial wilt pathogen Ralstonia solanacearum to efficiently invade and colonize host plants. However, the bacteria are essentially nonmotile once inside plant xylem vessels. To determine how and when motility genes are expressed, we cloned and mutated flhDC, which encodes a major regulator of flagellar biosynthesis and bacterial motility. An flhDC mutant was nonmotile and less virulent than its wild-type parent on both tomato and Arabidopsis; on Arabidopsis, the flhDC mutant also was less virulent than a nonmotile fliC flagellin mutant. Genes in the R. solanacearum motility regulon had strikingly different expression patterns in culture and in the plant. In culture, as expected, flhDC expression depended on PehSR, a regulator of early virulence factors; and, in turn, FlhDC was required for fliC (flagellin) expression. However, when bacteria grew in tomato plants, flhDC was expressed in both wild-type and pehR mutant backgrounds, although PehSR is necessary for motility both in culture and in planta. Both flhDC and pehSR were significantly induced in planta relative to expression levels in culture. Unexpectedly, the fliC gene was expressed in planta at cell densities where motile bacteria were not observed, as well as in a nonmotile flhDC mutant. Thus, expression of flhDC and flagellin itself are uncoupled from bacterial motility in the host environment, indicating that additional signals and regulatory circuits repress motility during plant pathogenesis.

  18. Revised Phylogeny and Novel Horizontally Acquired Virulence Determinants of the Model Soft Rot Phytopathogen Pectobacterium wasabiae SCC3193

    PubMed Central

    Koskinen, Patrik; Nokso-Koivisto, Jussi; Pasanen, Miia; Broberg, Martin; Plyusnin, Ilja; Törönen, Petri; Holm, Liisa; Pirhonen, Minna; Palva, E. Tapio

    2012-01-01

    Soft rot disease is economically one of the most devastating bacterial diseases affecting plants worldwide. In this study, we present novel insights into the phylogeny and virulence of the soft rot model Pectobacterium sp. SCC3193, which was isolated from a diseased potato stem in Finland in the early 1980s. Genomic approaches, including proteome and genome comparisons of all sequenced soft rot bacteria, revealed that SCC3193, previously included in the species Pectobacterium carotovorum, can now be more accurately classified as Pectobacterium wasabiae. Together with the recently revised phylogeny of a few P. carotovorum strains and an increasing number of studies on P. wasabiae, our work indicates that P. wasabiae has been unnoticed but present in potato fields worldwide. A combination of genomic approaches and in planta experiments identified features that separate SCC3193 and other P. wasabiae strains from the rest of soft rot bacteria, such as the absence of a type III secretion system that contributes to virulence of other soft rot species. Experimentally established virulence determinants include the putative transcriptional regulator SirB, two partially redundant type VI secretion systems and two horizontally acquired clusters (Vic1 and Vic2), which contain predicted virulence genes. Genome comparison also revealed other interesting traits that may be related to life in planta or other specific environmental conditions. These traits include a predicted benzoic acid/salicylic acid carboxyl methyltransferase of eukaryotic origin. The novelties found in this work indicate that soft rot bacteria have a reservoir of unknown traits that may be utilized in the poorly understood latent stage in planta. The genomic approaches and the comparison of the model strain SCC3193 to other sequenced Pectobacterium strains, including the type strain of P. wasabiae, provides a solid basis for further investigation of the virulence, distribution and phylogeny of soft rot

  19. Rice suspension cultured cells are evaluated as a model system to study salt responsive networks in plants using a combined proteomic and metabolomic profiling approach.

    PubMed

    Liu, Dawei; Ford, Kristina L; Roessner, Ute; Natera, Siria; Cassin, Andrew M; Patterson, John H; Bacic, Antony

    2013-06-01

    Salinity is one of the major abiotic stresses affecting plant productivity but surprisingly, a thorough understanding of the salt-responsive networks responsible for sustaining growth and maintaining crop yield remains a significant challenge. Rice suspension culture cells (SCCs), a single cell type, were evaluated as a model system as they provide a ready source of a homogenous cell type and avoid the complications of multicellular tissue types in planta. A combination of growth performance, and transcriptional analyses using known salt-induced genes was performed on control and 100 mM NaCl cultured cells to validate the biological system. Protein profiling was conducted using both DIGE- and iTRAQ-based proteomics approaches. In total, 106 proteins were identified in DIGE experiments and 521 proteins in iTRAQ experiments with 58 proteins common to both approaches. Metabolomic analysis provided insights into both developmental changes and salt-induced changes of rice SCCs at the metabolite level; 134 known metabolites were identified, including 30 amines and amides, 40 organic acids, 40 sugars, sugar acids and sugar alcohols, 21 fatty acids and sterols, and 3 miscellaneous compounds. Our results from proteomic and metabolomic studies indicate that the salt-responsive networks of rice SCCs are extremely complex and share some similarities with thee cellular responses observed in planta. For instance, carbohydrate and energy metabolism pathways, redox signaling pathways, auxin/indole-3-acetic acid pathways and biosynthesis pathways for osmoprotectants are all salt responsive in SCCs enabling cells to maintain cellular function under stress condition. These data are discussed in the context of our understanding of in planta salt-responses.

  20. Cathepsin B Cysteine Proteinase is Essential for the Development and Pathogenesis of the Plant Parasitic Nematode Radopholus similis.

    PubMed

    Li, Yu; Wang, Ke; Xie, Hui; Wang, Dong-Wei; Xu, Chun-Ling; Huang, Xin; Wu, Wen-Jia; Li, Dan-Lei

    2015-01-01

    Radopholus similis is an important plant parasitic nematode which severely harms many crops. Cathepsin B is present in a wide variety of organisms, and plays an important role in many parasites. Understanding cathepsin B of R. similis would allow us to find new targets and approaches for its control. In this study, we found that Rs-cb-1 mRNA was expressed in esophageal glands, intestines and gonads of females, testes of males, juveniles and eggs in R. similis. Rs-cb-1 expression was the highest in females, followed by juveniles and eggs, and was the lowest in males. The maximal enzyme activity of Rs-CB-1 was detected at pH 6.0 and 40 °C. Silencing of Rs-cb-1 using in vitro RNAi (Soaking with dsRNA in vitro) not only significantly inhibited the development and hatching of R. similis, but also greatly reduced its pathogenicity. Using in planta RNAi, we confirmed that Rs-cb-1 expression in nematodes were significantly suppressed and the resistance to R. similis was significantly improved in T2 generation transgenic tobacco plants expressing Rs-cb-1 dsRNA. The genetic effects of in planta RNAi-induced gene silencing could be maintained in the absence of dsRNA for at least two generations before being lost, which was not the case for the effects induced by in vitro RNAi. Overall, our results first indicate that Rs-cb-1 plays key roles in the development, hatching and pathogenesis of R. similis, and that in planta RNAi is an effective tool in studying gene function and genetic engineering of plant resistance to migratory plant parasitic nematodes.

  1. Target mimics: an embedded layer of microRNA-involved gene regulatory networks in plants

    PubMed Central

    2012-01-01

    Background MicroRNAs (miRNAs) play an essential role in gene regulation in plants. At the same time, the expression of miRNA genes is also tightly controlled. Recently, a novel mechanism called “target mimicry” was discovered, providing another layer for modulating miRNA activities. However, except for the artificial target mimics manipulated for functional studies on certain miRNA genes, only one example, IPS1 (Induced by Phosphate Starvation 1)—miR399 was experimentally confirmed in planta. To date, few analyses for comprehensive identification of natural target mimics have been performed in plants. Thus, limited evidences are available to provide detailed information for interrogating the questionable issue whether target mimicry was widespread in planta, and implicated in certain biological processes. Results In this study, genome-wide computational prediction of endogenous miRNA mimics was performed in Arabidopsis and rice, and dozens of target mimics were identified. In contrast to a recent report, the densities of target mimic sites were found to be much higher within the untranslated regions (UTRs) when compared to those within the coding sequences (CDSs) in both plants. Some novel sequence characteristics were observed for the miRNAs that were potentially regulated by the target mimics. GO (Gene Ontology) term enrichment analysis revealed some functional insights into the predicted mimics. After degradome sequencing data-based identification of miRNA targets, the regulatory networks constituted by target mimics, miRNAs and their downstream targets were constructed, and some intriguing subnetworks were further exploited. Conclusions These results together suggest that target mimicry may be widely implicated in regulating miRNA activities in planta, and we hope this study could expand the current understanding of miRNA-involved regulatory networks. PMID:22613869

  2. Involvement of hydrogen peroxide in leaf abscission signaling, revealed by analysis with an in vitro abscission system in Capsicum plants.

    PubMed

    Sakamoto, Masaru; Munemura, Ikuko; Tomita, Reiko; Kobayashi, Kappei

    2008-10-01

    Although auxin and ethylene play pivotal roles in leaf abscission, the subsequent signaling molecules are poorly understood. This is mainly because it is difficult to effectively treat the intact abscission zone (AZ) with pharmacological reagents. We developed an in vitro experimental system that reproduces stress-induced leaf abscission in planta. In this system, 1-mm-thick petiole strips, encompassing the AZ, were separated within 4 days of abscission at the AZ through cell wall degradation in an auxin depletion- and ethylene-dependent manner. The system allowed us to show that hydrogen peroxide (H(2)O(2)) is involved in abscission signaling. Microscopic analyses revealed continuous H(2)O(2) production by AZ cells. H(2)O(2) scavengers and diphenylene iodonium, an inhibitor of NADPH oxidase, suppressed in vitro abscission and cellulase expression. Conversely, the application of H(2)O(2) promoted in vitro abscission and expression of cellulase. Ethephon-induced abscission was suppressed by inhibitors of H(2)O(2) production, whereas the expression of ethylene-responsive genes was unaffected by both H(2)O(2) and an H(2)O(2) inhibitor. These results indicated that H(2)O(2) acts downstream from ethylene in in vitro abscission signaling. In planta, salinity stress induced the expression of genes that respond to ethylene and reactive oxygen species, and also induced H(2)O(2) production at the AZ, which preceded leaf abscission. These results indicate that H(2)O(2) has roles in leaf abscission associated with ethylene both in vitro and in planta.

  3. Revised phylogeny and novel horizontally acquired virulence determinants of the model soft rot phytopathogen Pectobacterium wasabiae SCC3193.

    PubMed

    Nykyri, Johanna; Niemi, Outi; Koskinen, Patrik; Nokso-Koivisto, Jussi; Pasanen, Miia; Broberg, Martin; Plyusnin, Ilja; Törönen, Petri; Holm, Liisa; Pirhonen, Minna; Palva, E Tapio

    2012-01-01

    Soft rot disease is economically one of the most devastating bacterial diseases affecting plants worldwide. In this study, we present novel insights into the phylogeny and virulence of the soft rot model Pectobacterium sp. SCC3193, which was isolated from a diseased potato stem in Finland in the early 1980s. Genomic approaches, including proteome and genome comparisons of all sequenced soft rot bacteria, revealed that SCC3193, previously included in the species Pectobacterium carotovorum, can now be more accurately classified as Pectobacterium wasabiae. Together with the recently revised phylogeny of a few P. carotovorum strains and an increasing number of studies on P. wasabiae, our work indicates that P. wasabiae has been unnoticed but present in potato fields worldwide. A combination of genomic approaches and in planta experiments identified features that separate SCC3193 and other P. wasabiae strains from the rest of soft rot bacteria, such as the absence of a type III secretion system that contributes to virulence of other soft rot species. Experimentally established virulence determinants include the putative transcriptional regulator SirB, two partially redundant type VI secretion systems and two horizontally acquired clusters (Vic1 and Vic2), which contain predicted virulence genes. Genome comparison also revealed other interesting traits that may be related to life in planta or other specific environmental conditions. These traits include a predicted benzoic acid/salicylic acid carboxyl methyltransferase of eukaryotic origin. The novelties found in this work indicate that soft rot bacteria have a reservoir of unknown traits that may be utilized in the poorly understood latent stage in planta. The genomic approaches and the comparison of the model strain SCC3193 to other sequenced Pectobacterium strains, including the type strain of P. wasabiae, provides a solid basis for further investigation of the virulence, distribution and phylogeny of soft rot

  4. Constitutive expression of fluorescent protein by Aspergillus var. niger and Aspergillus carbonarius to monitor fungal colonization in maize plants.

    PubMed

    Palencia, Edwin Rene; Glenn, Anthony Elbie; Hinton, Dorothy Mae; Bacon, Charles Wilson

    2013-09-01

    Aspergillus niger and Aspergillus carbonarius are two species in the Aspergillus section Nigri (black-spored aspergilli) frequently associated with peanut (Arachis hypogea), maize (Zea mays), and other plants as pathogens. These infections are symptomless and as such are major concerns since some black aspergilli produce important mycotoxins, ochratoxins A, and the fumonisins. To facilitate the study of the black aspergilli-maize interactions with maize during the early stages of infections, we developed a method that used the enhanced yellow fluorescent protein (eYFP) and the monomeric red fluorescent protein (mRFP1) to transform A. niger and A. carbonarius, respectively. The results were constitutive expressions of the fluorescent genes that were stable in the cytoplasms of hyphae and conidia under natural environmental conditions. The hyphal in planta distribution in 21-day-old seedlings of maize were similar wild type and transformants of A. niger and A. carbonarius. The in planta studies indicated that both wild type and transformants internally colonized leaf, stem and root tissues of maize seedlings, without any visible disease symptoms. Yellow and red fluorescent strains were capable of invading epidermal cells of maize roots intercellularly within the first 3 days after inoculation, but intracellular hyphal growth was more evident after 7 days of inoculation. We also tested the capacity of fluorescent transformants to produce ochratoxin A and the results with A. carbonarius showed that this transgenic strain produced similar concentrations of this secondary metabolite. This is the first report on the in planta expression of fluorescent proteins that should be useful to study the internal plant colonization patterns of two ochratoxigenic species in the Aspergillus section Nigri.

  5. Lipid labeling from acetate or glycerol in cultured embryos of Camelina sativa seeds: A tale of two substrates.

    PubMed

    Pollard, Mike; Delamarter, Danielle; Martin, Tina M; Shachar-Hill, Yair

    2015-10-01

    Studies on the metabolism of lipids in seeds frequently use radiolabeled acetate and glycerol supplied to excised developing seeds to track the biosynthesis of acyl and lipid head groups, respectively. Such experiments are generally restricted to shorter time periods and the results may not quantitatively reflect in planta rates. These limitations can be removed by using cultured embryos, provided they mimic growth and lipid deposition observed for embryos in planta. Mid-maturation embryos from Camelina sativa were cultured in vitro to assess the use of sufficient acetate or glycerol concentrations and labeling periods for stable isotope labeling and mass spectrometric detection. Maximum incorporation of exogenous acetate into fatty acids occurred at 1mM and above. This provides about 5% of the total carbon flux entering fatty acids, enough for (13)C isotopomer analysis while maintaining normal biosynthetic rates for over 24h. Labeling analysis indicates that acetate reports lipid metabolism uniformly across the embryo. At higher acetate concentrations with longer incubations, the rate of fatty acid synthesis is reduced and the composition of newly synthesized fatty acids changes. While the mole fractions of oleate that undergo Δ12-desaturation or elongation are independent of biosynthetic flux, Δ15-desaturation shows a bimodal dependence. These observations are consistent with changes occurring in planta over seed development. Incorporation rates of the glyceryl moiety into lipids saturates at about 0.5mM exogenous glycerol. At saturation, the exogenous glycerol almost completely replaces the endogenous supply of glycerol-3-phosphate without affecting net lipid accumulation or fatty acid composition. It is concluded that acetate and glycerol labeling of cultured C. sativa embryos can provide an accurate representation of lipid metabolism in embryos in vivo, and that in Camelina embryos glycerol-3-phosphate levels do not co-limit triacylglycerol synthesis.

  6. Cathepsin B Cysteine Proteinase is Essential for the Development and Pathogenesis of the Plant Parasitic Nematode Radopholus similis

    PubMed Central

    Li, Yu; Wang, Ke; Xie, Hui; Wang, Dong-Wei; Xu, Chun-Ling; Huang, Xin; Wu, Wen-Jia; Li, Dan-Lei

    2015-01-01

    Radopholus similis is an important plant parasitic nematode which severely harms many crops. Cathepsin B is present in a wide variety of organisms, and plays an important role in many parasites. Understanding cathepsin B of R. similis would allow us to find new targets and approaches for its control. In this study, we found that Rs-cb-1 mRNA was expressed in esophageal glands, intestines and gonads of females, testes of males, juveniles and eggs in R. similis. Rs-cb-1 expression was the highest in females, followed by juveniles and eggs, and was the lowest in males. The maximal enzyme activity of Rs-CB-1 was detected at pH 6.0 and 40 °C. Silencing of Rs-cb-1 using in vitro RNAi (Soaking with dsRNA in vitro) not only significantly inhibited the development and hatching of R. similis, but also greatly reduced its pathogenicity. Using in planta RNAi, we confirmed that Rs-cb-1 expression in nematodes were significantly suppressed and the resistance to R. similis was significantly improved in T2 generation transgenic tobacco plants expressing Rs-cb-1 dsRNA. The genetic effects of in planta RNAi-induced gene silencing could be maintained in the absence of dsRNA for at least two generations before being lost, which was not the case for the effects induced by in vitro RNAi. Overall, our results first indicate that Rs-cb-1 plays key roles in the development, hatching and pathogenesis of R. similis, and that in planta RNAi is an effective tool in studying gene function and genetic engineering of plant resistance to migratory plant parasitic nematodes. PMID:26221074

  7. Xanthoferrin, the α-hydroxycarboxylate-type siderophore of Xanthomonas campestris pv. campestris, is required for optimum virulence and growth inside cabbage.

    PubMed

    Pandey, Sheo Shankar; Patnana, Pradeep Kumar; Rai, Rikky; Chatterjee, Subhadeep

    2016-06-27

    Xanthomonas campestris pv. campestris causes black rot, a serious disease of crucifers. Xanthomonads encode a siderophore biosynthesis and uptake gene cluster xss (Xanthomonas siderophore synthesis) involved in the production of a vibrioferrin-type siderophore. However, little is known about the role of the siderophore in the iron uptake and virulence of X. campestris pv. campestris. In this study, we show that X. campestris pv. campestris produces an α-hydroxycarboxylate-type siderophore (named xanthoferrin), which is required for growth under low-iron conditions and for optimum virulence. A mutation in the siderophore synthesis xssA gene causes deficiency in siderophore production and growth under low-iron conditions. In contrast, the siderophore utilization ΔxsuA mutant is able to produce siderophore, but exhibits a defect in the utilization of the siderophore-iron complex. Our radiolabelled iron uptake studies confirm that the ΔxssA and ΔxsuA mutants exhibit defects in ferric iron (Fe(3+) ) uptake. The ΔxssA mutant is able to utilize and transport the exogenous xanthoferrin-Fe(3+) complex; in contrast, the siderophore utilization or uptake mutant ΔxsuA exhibits defects in siderophore uptake. Expression analysis of the xss operon using a chromosomal gusA fusion indicates that the xss operon is expressed during in planta growth and under low-iron conditions. Furthermore, exogenous iron supplementation in cabbage leaves rescues the in planta growth deficiency of ΔxssA and ΔxsuA mutants. Our study reveals that the siderophore xanthoferrin is an important virulence factor of X. campestris pv. campestris which promotes in planta growth by the sequestration of Fe(3+) .

  8. Gene sampling can bias multi-gene phylogenetic inferences: the relationship between red algae and green plants as a case study.

    PubMed

    Inagaki, Yuji; Nakajima, Yoshihiro; Sato, Mitsuhisa; Sakaguchi, Miako; Hashimoto, Tetsuo

    2009-05-01

    The monophyly of Plantae including glaucophytes, red algae, and green plants (green algae plus land plants) has been recovered in recent phylogenetic analyses of large multi-gene data sets (e.g., those including >30,000 amino acid [aa] positions). On the other hand, Plantae monophyly has not been stably reconstructed in inferences from multi-gene data sets with fewer than 10,000 aa positions. An analysis of 5,216 aa positions in Nozaki et al. (Nozaki H, Iseki M, Hasegawa M, Misawa K, Nakada T, Sasaki N, Watanabe M. 2007. Phylogeny of primary photosynthetic eukaryotes as deduced from slowly evolving nuclear genes. Mol Biol Evol. 24:1592-1595.) strongly rejected the monophyly of Plantae, whereas Hackett et al. (Hackett JD, Yoon HS, Li S, Reyes-Prieto A, Rummele SE, Bhattacharya D. 2007. Phylogenomic analysis supports the monophyly of cryptophytes and haptophytes and the association of rhizaria with chromalveolates. Mol Biol Evol. 24:1702-1713.) robustly recovered the Plantae clade in an analysis of 6,735 aa positions. We suspected that the significant incongruity observed between the two studies was attributable to a bias generally overlooked in multi-gene phylogenetic estimation, rather than data size, taxon sampling, or methods for tree reconstruction. Although glaucophytes were excluded from our analyses due to a shortage of sequence data, we found that the recovery of a sister-group relationship between red algae and green plants primarily depends on gene sampling in phylogenetic inferences from <10,000 aa positions. Phylogenetic analyses of data sets with fewer than 10,000 aa positions, which can be prepared without large-scale sequencing (e.g., expressed sequence tag analyses), are practical in challenging various unresolved issues in eukaryotic evolution. However, our results indicate that severe biases can arise from gene sampling in multi-gene inferences from <10,000 aa positions. We also address the validity of fast-evolving gene exclusion in multi

  9. Archaeal-eubacterial mergers in the origin of Eukarya: phylogenetic classification of life.

    PubMed Central

    Margulis, L

    1996-01-01

    A symbiosis-based phylogeny leads to a consistent, useful classification system for all life. "Kingdoms" and "Domains" are replaced by biological names for the most inclusive taxa: Prokarya (bacteria) and Eukarya (symbiosis-derived nucleated organisms). The earliest Eukarya, anaerobic mastigotes, hypothetically originated from permanent whole-cell fusion between members of Archaea (e.g., Thermoplasma-like organisms) and of Eubacteria (e.g., Spirochaeta-like organisms). Molecular biology, life-history, and fossil record evidence support the reunification of bacteria as Prokarya while subdividing Eukarya into uniquely defined subtaxa: Protoctista, Animalia, Fungi, and Plantae. Images Fig. 1 PMID:8577716

  10. Archaeal-eubacterial mergers in the origin of Eukarya: phylogenetic classification of life.

    PubMed

    Margulis, L

    1996-02-06

    A symbiosis-based phylogeny leads to a consistent, useful classification system for all life. "Kingdoms" and "Domains" are replaced by biological names for the most inclusive taxa: Prokarya (bacteria) and Eukarya (symbiosis-derived nucleated organisms). The earliest Eukarya, anaerobic mastigotes, hypothetically originated from permanent whole-cell fusion between members of Archaea (e.g., Thermoplasma-like organisms) and of Eubacteria (e.g., Spirochaeta-like organisms). Molecular biology, life-history, and fossil record evidence support the reunification of bacteria as Prokarya while subdividing Eukarya into uniquely defined subtaxa: Protoctista, Animalia, Fungi, and Plantae.

  11. Cytokinin Transporters: GO and STOP in Signaling.

    PubMed

    Kang, Joohyun; Lee, Youngsook; Sakakibara, Hitoshi; Martinoia, Enrico

    2017-03-31

    Cytokinins are phytohormones essential for cytokinesis and many other physiological and developmental processes in planta. Long-distance transport and intercellular transport have been postulated. For these processes, the existence of cytokinin transporters has been suggested. Recently, a transporter loading the xylem (AtABCG14) and another for cellular import (AtPUP14) have been discovered. AtABCG14 participates in the xylem loading process of cytokinins and contributes to the positive regulation of shoot growth. The cellular importer AtPUP14 is required to suppress cytokinin signaling. A role of a transporter as stop signal is a new paradigm for a hormone transporter.

  12. Dual Effect of the Cubic Ag3PO4 Crystal on Pseudomonas syringae Growth and Plant Immunity

    PubMed Central

    Kim, Mi Kyung; Yeo, Byul-Ee; Park, Heonyong; Huh, Young-Duk; Kwon, Chian; Yun, Hye Sup

    2016-01-01

    We previously found that the antibacterial activity of silver phosphate crystals on Escherichia coli depends on their structure. We here show that the cubic form of silver phosphate crystal (SPC) can also be applied to inhibit the growth of a plant-pathogenic Pseudomonas syringae bacterium. SPC pretreatment resulted in reduced in planta multiplication of P. syringae. Induced expression of a plant defense marker gene PR1 by SPC alone is suggestive of its additional plant immunity-stimulating activity. Since SPC can simultaneously inhibit P. syringae growth and induce plant defense responses, it might be used as a more effective plant disease-controlling agent. PMID:27147937

  13. Antioxidant responses of damiana (Turnera diffusa Willd) to exposure to artificial ultraviolet (UV) radiation in an in vitro model; part I; UV-C radiation.

    PubMed

    Soriano-Melgar, Lluvia de Abril Alexandra; Alcaraz-Meléndez, Lilia; Méndez-Rodríguez, Lía C; Puente, María Esther; Rivera-Cabrera, Fernando; Zenteno-Savín, Tania

    2014-05-01

    Introducción: La radiación ultravioleta tipo C (UV-C) presenta mayor energía y es menos estudiada que la radiación UV-B, debido a que se considera que es totalmente absorbida por la capa de ozono. Sin embargo, la radiación UV-C artificial es capaz de generar diversas modificaciones en las plantas. Dado que la exposición a UV-C por intervalos de tiempo cortos incrementa la concentración de compuestos antioxidantes, mejorando la apariencia y vida de anaquel de los productos, su potencial aplicación en tratamientos poscosecha para modificar el contenido antioxidante de plantas medicinales, como la damiana (Turnera diffusa), es novedoso y relevante. Objetivo: Determinar el efecto de la radiación UV-C sobre las defensas antioxidantes enzimáticas y no enzimáticas, así como en los niveles de daño oxidativo de damiana (Turnera diffusa) in vitro. Resultados: La radiación UV-C disminuyó la actividad de las enzimas superóxido dismutasa (SOD, EC 1.15.1.1) y peroxidasas totales (POX, CE 1.11.1), la concentración de clorofila (a y b), carotenos, vitamina C y la capacidad antioxidante total, e incrementó el contenido de compuestos fenólicos en damiana. La disminución de las defensas antioxidantes fue mayor en plantas de damiana expuestas a dosis más altas de UV-C o por períodos más largos. Estos resultados sugieren que la radiación UV-C induce estrés oxidativo, evidenciado por el incremento del contenido de carbonilos proteicos y el contenido de compuestos fenólicos en damiana (T. diffusa). Conclusión: Dosis bajas y menor exposición a UV-C estimulan la síntesis de compuestos fenólicos en damiana. Por ello, tratamientos controlados con UV-C podrían emplearse como tratamientos poscosecha para incrementar el contenido de compuestos fenólicos en plantas de damiana.

  14. Pilot study of bovine interdigital cassetteless computed radiography.

    PubMed

    El-Shafaey, El-Sayed Ahmed Awad; Aoki, Takahiro; Ishii, Mitsuo; Yamada, Kazutaka

    2013-11-01

    Twenty-one limbs of bovine cadavers (42 digits) were exposed to interdigital cassetteless imaging plate using computed radiography. The radiographic findings included exostosis, a rough planta surface, osteolysis of the apex of the distal phalanx and widening of the laminar zone between the distal phalanx and the hoof wall. All these findings were confirmed by computed tomography. The hindlimbs (19 digits) showed more changes than the forelimbs (10 digits), particularly in the lateral distal phalanx. The cassetteless computed radiography technique is expected to be an easily applicable method for the distal phalanx rather than a conventional cassette-plate and/or the film-screen cassetteless methods.

  15. Isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase from leaves.

    PubMed

    Carmo-Silva, A Elizabete; Barta, Csengele; Salvucci, Michael E

    2011-01-01

    Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a multifunctional enzyme that catalyzes the fixation of CO2 and O2 in photosynthesis and photorespiration, respectively. As the rate-limiting step in photosynthesis, improving the catalytic properties of Rubisco has long been viewed as a viable strategy for increasing plant productivity. Advances in biotechnology have made this goal more attainable by making it possible to modify Rubisco in planta. To properly evaluate the properties of Rubisco, it is necessary to isolate the enzyme in pure form. This chapter describes procedures for rapid and efficient purification of Rubisco from leaves of several species.

  16. Laser-Based Methods for Detection of Nitric Oxide in Plants.

    PubMed

    Mandon, Julien; Mur, Luis A J; Harren, Frans J M; Cristescu, Simona M

    2016-01-01

    Nitric oxide (NO) plays an important role in plant signaling and in response to various stress conditions. Therefore, real-time measurements of NO production provide better insights into understanding plant processes and can help developing strategies to improve food production and postharvest quality. Using laser-based spectroscopic methods, sensitive, online, in planta measurements of plant-pathogen interactions are possible. This chapter introduces the basic principle of the optical detectors using different laser sources for accurate monitoring of fast dynamic changes of NO production. Several applications are also presented to demonstrate the suitability of these detectors for detection of NO in plants.

  17. Analysis of Unfolded Protein Response in Arabidopsis

    PubMed Central

    Chen, Yani; Brandizzi, Federica

    2014-01-01

    The unfolded protein response (UPR) is fundamental for development and adaption in eukaryotic cells. Arabidopsis has become one of the best model systems to uncover conserved mechanisms of the UPR in multicellular eukaryotes as well as organism-specific regulation of the UPR in plants. Monitoring the UPR in planta is an elemental approach to identifying regulatory components and to revealing molecular mechanisms of the plant UPR. In this chapter, we provide protocols for the induction and analyses of plant UPR at a molecular level in Arabidopsis. Three kinds of ER stress treatment methods and quantitation of the plant UPR activation are described here. PMID:23913037

  18. Relationship of Estuarine Plant Contaminants to Existing Data Bases

    DTIC Science & Technology

    1988-02-01

    Experiments. Plant and Soil, ?8: 271-282. COMISSAO NACIONAL DO AMBIENTE (1980). Atlas do Ambiente de Portugal.CNA, Lisboa. CROWDER, A.A. & Macfie, S.M...Photo- synthetica, 18: 134-138. INSTITUTO AGRONONICO DE S. PAULO (1978). Andlise Quimica de Plantas.Cir cular N2 87, Sic Paulo. KIEKENS, L. Cottenie, A...Estudos de Am- biente, Lisboa. PENEDA, M.C. & Coelho, A.T. (1978). Valor e Potencialidade8 do Estudrio do Sado. ServiCo de Estudos de Ambiente , Lisboa

  19. Archaeal-eubacterial mergers in the origin of Eukarya: phylogenetic classification of life

    NASA Technical Reports Server (NTRS)

    Margulis, L.

    1996-01-01

    A symbiosis-based phylogeny leads to a consistent, useful classification system for all life. "Kingdoms" and "Domains" are replaced by biological names for the most inclusive taxa: Prokarya (bacteria) and Eukarya (symbiosis-derived nucleated organisms). The earliest Eukarya, anaerobic mastigotes, hypothetically originated from permanent whole-cell fusion between members of Archaea (e.g., Thermoplasma-like organisms) and of Eubacteria (e.g., Spirochaeta-like organisms). Molecular biology, life-history, and fossil record evidence support the reunification of bacteria as Prokarya while subdividing Eukarya into uniquely defined subtaxa: Protoctista, Animalia, Fungi, and Plantae.

  20. Authoritarian Decision-Making and Alternative Patterns of Power and Influence: The Mexican IBM Case

    DTIC Science & Technology

    1988-05-01

    Mercado de Valores 44, 37 (10 September 1984), pp. 926-929. 120. See Jaime Contreras, "Rechazo la CNIE la Solicitud de la IBM Para Instalar una...Interview with Alejandro del Toro, 5 March 1987. 65. Rodolfo Guerrero, "El Ingreso de IBM a Mexico generara divisas, empleos y desarollo: M. Kun...Jaime. "Rechazo la CNIE la SolIcitud de la IBM Para Instalar una Planta de Computadoras."Excelsior, 28 January 1985. El Financiero, "IBM Intends to