Donges Receives 2013 Donald L. Turcotte Award

NASA Astrophysics Data System (ADS)

2014-07-01

Jonathan F. Donges was awarded the 2013 Donald L. Turcotte Award, given annually to recent Ph.D. recipients for outstanding dissertation research that contributes directly to the field of nonlinear geophysics. Donges's Ph.D. thesis is entitled "Functional network macroscopes for probing past and present Earth system dynamics." He gave an invited talk and was formally presented with the award at the 2013 AGU Fall Meeting, held 9-13 December in San Francisco, Calif.

HbA1c is significantly associated with arterial stiffness but not with carotid atherosclerosis in a community-based population without type 2 diabetes: The Dong-gu study.

PubMed

Lee, Young-Hoon; Shin, Min-Ho; Choi, Jin-Su; Rhee, Jung-Ae; Nam, Hae-Sung; Jeong, Seul-Ki; Park, Kyeong-Soo; Ryu, So-Yeon; Choi, Seong-Woo; Kim, Bok-Hee; Oh, Gyung-Jae; Kweon, Sun-Seog

2016-04-01

We examined the associations between HbA1c levels and various atherosclerotic vascular parameters among adults without diabetes from the general population. A total of 6500 community-dwelling adults, who were free of type 2 diabetes and ≥50 years of age, were included. High-resolution B-mode ultrasound was used to evaluate carotid artery structure, including intima-media thickness (IMT), plaque, and luminal diameter. Brachial-ankle pulse wave velocity (baPWV), which is a useful indicator of systemic arterial stiffness, was determined using an automatic waveform analysis device. No significant associations were observed between HbA1c, carotid IMT, plaque, or luminal diameter in a fully adjusted model. However, the odds ratio (95% confidence interval) for high baPWV (defined as the highest quartile) increased by 1.43 (1.19-1.71) per 1% HbA1c increase after adjusting for conventional risk factors in a multivariate logistic regression analysis. In addition, HbA1c was independently associated with baPWV in a multivariate linear regression analysis. High-normal HbA1c level was independently associated with arterial stiffness, but not with carotid atherosclerotic parameters, in the general population without diabetes. Our results suggest that the functional atherosclerotic process may already be accelerated according to HbA1c level, even at a level below the diagnostic threshold for diabetes. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Characteristics of Navy Medium-Weight High-Impact Shock Machine

DTIC Science & Technology

1951-09-14

Ar 2-4~ -C 0 ofoL "- oili 0jit -1 b. V’ A . -- c- MC a y - w CmLUA ~ E~8.9. - flb. A 9 Er IL v II - W43 4P " It IIf ~NRL REPORT I CHARACTERISTICS OF...this machine under specification operation. A comnarison of datta is intended to correlate this shock to shipboard shock experienced in actual combat...table reversal are discussed, and it is shown that this secondsry shock can be the largest under certain condition. Theoretical equations of motion are

15 CFR Supplement No. 7 to Part 748 - Authorization Validated End-User (VEU): List of Validated End-Users, Respective Items Eligible...

Code of Federal Regulations, 2010 CFR

2010-01-01

...; 2B230; 2B350.d.2 2B350.g.3 2B350.i.4; 3B001.c.2; 3C002; 3C004 Headquarters and Fab. 1 of HHNEC, No. 1188 Chuan Qiao Rd., Pu Dong, Shanghai, China 201206.Fab. 2 of HHNEC, No. 668 Guo Shou Jing Rd., Zhang Jiang...

Principles of Work Sample Testing. 4. Generalizability

DTIC Science & Technology

1979-04-01

ARI TECHNICAL REPORT TR-79-A11 Principles of Work Sample Testing: IV. Generallzability , by ,lobert M. Guion Gail H. Ironson BOWLING GREEN STATE...UNIVERSITY Bowling Green , Ohio 43403 April 1979 or -Contract DAHC 19-77-C-0007 d CD, LUa.J Prepared for -_J ;=U.S. ARMY RESEARCH INSTITUTE for the...ORGANIZATION NAME AND ADDRESS 10. PROG RAM ELEMENT. PROJECT, TASK A REA & WORK UNIT NUMBERS Bowling Green State University __ Bowling Green , Ohio 43403

Trapping systems for Zaprionus indianus (Diptera: Drosophilidae)

USDA-ARS?s Scientific Manuscript database

62nd Annual Meeting of the Entomological Society of America 16-19 November 2014; Portland, OR Title: Trapping systems for Zaprionus indianus (Diptera: Drosophilidae) Nancy D. Epsky, Micah A. Gill, C. Teri Allen, Dong H. Cha, and Peter J. Landolt Nancy D. Epsky USDA-ARS, Subtropical Horticulture...

Effect of progressive drought stress on growth, leaf gas exchange, and antioxidant production in two maize cultivars.

PubMed

Anjum, Shakeel Ahmad; Tanveer, Mohsin; Ashraf, Umair; Hussain, Saddam; Shahzad, Babar; Khan, Imran; Wang, Longchang

2016-09-01

Drought stress is one of the major environmental factors responsible for reduction in crop productivity. In the present study, responses of two maize cultivars (Rung Nong 35 and Dong Dan 80) were examined to explicate the growth, yield, leaf gas exchange, leaf water contents, osmolyte accumulation, membrane lipid peroxidation, and antioxidant activity under progressive drought stress. Maize cultivars were subjected to varying field capacities (FC) viz., well-watered (80 % FC) and drought-stressed (35 % FC) at 45 days after sowing. The effects of drought stress were analyzed at 5, 10, 15, 20, ad 25 days after drought stress (DAS) imposition. Under prolonged drought stress, Rung Nong 35 exhibited higher reduction in growth and yield as compared to Dong Dan 80. Maize cultivar Dong Dan 80 showed higher leaf relative water content (RWC), free proline, and total carbohydrate accumulation than Run Nong 35. Malondialdehyde (MDA) and superoxide anion were increased with prolongation of drought stress, with higher rates in cultivar Run Nong 35 than cultivar Dong Dan 80. Higher production of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) and glutathione reductase (GR) resulted in improved growth and yield in Dong Dan 80. Overall, the cultivar Dong Dan 80 was better able to resist the detrimental effects of progressive drought stress as indicated by better growth and yield due to higher antioxidant enzymes, reduced lipid peroxidation, better accumulation of osmolytes, and maintenance of tissue water contents.

Functional Analysis of Frequent Lung Cancer Amplicons Under Current Genome Annotation

DTIC Science & Technology

2015-12-01

Gingeras, T. R ., Rosenbloom, K. R ., Sloan, C. A., Learned, K., Malladi , V. S., Wong, M. C., Barber, G. P., Cline, M. S., Dreszer, T. R ., Heitner, S...Epstein, C. B., Frietze, S., Harrow, J., Kaul, R ., Khatun, J., Lajoie, B. R ., Landt, S. G., Lee, B. K., Pauli, F., Rosenbloom, K. R ., Sabo, P...Safi, A., Sanyal, A., Shoresh, N., Simon, J. M., Song, L., Trinklein, N. D., Altshuler, R . C., Birney, E., Brown, J. B., Cheng, C., Djebali, S., Dong

Dong Quai

MedlinePlus

... who had a stroke. Allergies. Anemia. Constipation. Early orgasm in men (premature ejaculation). High blood pressure. Joint aches and pains. Migraine ... 3 months. APPLIED TO THE SKIN: For early orgasm in men (premature ejaculation): A specific cream containing Panax ginseng root, dong ...

Assessment of Young Dong tributary and Imgok Creek impacted by Young Dong coal mine, South Korea.

PubMed

Lee, Byung-Tae; Ranville, James F; Wildeman, Thomas R; Jang, Min; Shim, Yon Sik; Ji, Won Hyun; Park, Hyun Sung; Lee, Hyun Ju

2012-01-01

An initial reclamation of the Young Dong coal mine site, located in northeastern South Korea, was completed in 1995. Despite the filling of the adit with limestone, acid rock drainage (ARD) enters Young Dong tributary and is then discharged to Imgok Creek. This ARD carries an average of 500 mg CaCO(3)/l of mineral acidity, primarily as Fe(II) and Al. Before spring runoff, the flow of Imgok Creek is 3.3-4 times greater than that of the tributary and has an alkalinity of 100 mg CaCO(3)/l, which is sufficient to eliminate the mineral acidity and raise the pH to about 6.5. From April through September 2008, there were at least two periods of high surface flow that affects the flow of ARD from the adit. Flow of ARD reaches 2.8 m(3)/min during spring runoff. This raised the concentrations of Fe and Al in the confluence with Imgok Creek. However, by 2 km downstream the pH of the Imgok Creek is 6.5 and only dissolved Fe is above the Korean drinking water criteria (0.30 mg/l). This suggests only a minor impact of Young Dong Creek water on Imgok Creek. Acid digestion of the sediments in Imgok Creek and Young Dong Tributary reveals considerable abundances of heavy metals, which could have a long-term impact on water quality. However, several water-based leaching tests, which better simulate the bioavailable metals pool, released only Al, Fe, Mn, and Zn at concentrations exceeding the criteria for drinking water or aquatic life.

GPC: General Polygon Clipper library

NASA Astrophysics Data System (ADS)

Murta, Alan

2015-12-01

The University of Manchester GPC library is a flexible and highly robust polygon set operations library for use with C, C#, Delphi, Java, Perl, Python, Haskell, Lua, VB.Net and other applications. It supports difference, intersection, exclusive-or and union clip operations, and polygons may be comprised of multiple disjoint contours. Contour vertices may be given in any order - clockwise or anticlockwise, and contours may be convex, concave or self-intersecting, and may be nested (i.e. polygons may have holes). Output may take the form of either polygon contours or tristrips, and hole and external contours are differentiated in the result.

Growth rate and surfactant-assisted enhancements of rare-earth arsenide InGaAs nanocomposites for terahertz generation

NASA Astrophysics Data System (ADS)

Salas, R.; Guchhait, S.; Sifferman, S. D.; McNicholas, K. M.; Dasika, V. D.; Jung, D.; Krivoy, E. M.; Lee, M. L.; Bank, S. R.

2017-09-01

We report the effects of the growth rate on the properties of iii-v nanocomposites containing rare-earth-monopnictide nanoparticles. In particular, the beneficial effects of surfactant-assisted growth of LuAs:In0.53Ga0.47As nanocomposites were found to be most profound at reduced LuAs growth rates. Substantial enhancement in the electrical and optical properties that are beneficial for ultrafast photoconductors was observed and is attributed to the higher structural quality of the InGaAs matrix in this new growth regime. The combined enhancements enabled a >50% increase in the amount of LuAs that could be grown without degrading the quality of the InGaAs overgrowth. Dark resistivity increased by ˜25× while maintaining carrier mobilities over 3000 cm2/V s; carrier lifetimes were reduced by >2×, even at high depositions of LuAs. The combined growth rate and surfactant enhancements offer a previously unexplored regime to enable high-performance fast photoconductors that may be integrated with telecom components for compact, broadly tunable, heterodyne THz source and detectors.

In vitro immunomodulatory effects of herbal products.

PubMed

Wilasrusmee, Chumpon; Siddiqui, Josephine; Bruch, David; Wilasrusmee, Skuntala; Kittur, Smita; Kittur, Dilip S

2002-10-01

Immunosuppressive drugs have been developed from natural products such as soil and fungi, which are also the sources of some commonly used herbal products. However, the effect of herbal products on immune response has not been investigated. Because these products can affect the host immune system they can induce either rejection or tolerance after a transplant procedure. To investigate the effects of ten commonly used herbal products on transplant-related immune function we performed in vitro lymphocyte proliferation tests using phytohemagglutinin, mixed lymphocyte culture (MLC) assay, and interleukin (IL)-2 and IL-10 production from MLC. Dong quai, ginseng, and milk thistle had nonspecific immunostimulatory effects on lymphocyte proliferation, whereas ginger and green tea had immunosuppressive effects. Dong quai and milk thistle increased alloresponsiveness in MLC, whereas ginger and tea decreased these responses. The immunostimulatory effects of dong quai and milk thistle were consistently seen in both cell-mediated immune response and nonspecific lymphoproliferation, whereas that of ginseng was not. The immunosuppressive effect of green tea and ginger were mediated through a decrease in IL-2 production, but the immunostimulatory effects of dong quai and milk thistle were not. We conclude that green tea, dong quai, ginseng, milk thistle, and ginger have effects on in vitro immune assays that may be relevant in transplantation in humans.

Endoplasmic Reticulum Stress as a Mediator of Neurotoxin-Induced Dopamine Neuron Death

DTIC Science & Technology

2007-07-01

the pathogenesis and treatment of disease. Science 267, 1456−1462. Tournier, C., Dong, C., Turner, T. K., Jones, S . N., Flavell, R. A., & Davis, R. J...are those of the author( s ) and should not be construed as an official Department of the Army position, policy or decision unless so designated by...Stress as a Mediator of Neurotoxin-Induced Dopamine Neuron Death 5b. GRANT NUMBER DAMD17-03-1-0492 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR( S ) 5d

Detecting Long-term Trend of Water Quality Indices of Dong-gang River, Taiwan Using Quantile Regression

NASA Astrophysics Data System (ADS)

Yang, D.; Shiau, J.

2013-12-01

ABSTRACT BODY: Abstract Surface water quality is an essential issue in water-supply for human uses and sustaining healthy ecosystem of rivers. However, water quality of rivers is easily influenced by anthropogenic activities such as urban development and wastewater disposal. Long-term monitoring of water quality can assess whether water quality of rivers deteriorates or not. Taiwan is a population-dense area and heavily depends on surface water for domestic, industrial, and agricultural uses. Dong-gang River is one of major resources in southern Taiwan for agricultural requirements. The water-quality data of four monitoring stations of the Dong-gang River for the period of 2000-2012 are selected for trend analysis. The parameters used to characterize water quality of rivers include biochemical oxygen demand (BOD), dissolved oxygen (DO), suspended solids (SS), and ammonia nitrogen (NH3-N). These four water-quality parameters are integrated into an index called river pollution index (RPI) to indicate the pollution level of rivers. Although widely used non-parametric Mann-Kendall test and linear regression exhibit computational efficiency to identify trends of water-quality indices, limitations of such approaches include sensitive to outliers and estimations of conditional mean only. Quantile regression, capable of identifying changes over time of any percentile values, is employed in this study to detect long-term trend of water-quality indices for the Dong-gang River located in southern Taiwan. The results show that Dong-gang River 4 stations from 2000 to 2012 monthly long-term trends in water quality.To analyze s Dong-gang River long-term water quality trends and pollution characteristics. The results showed that the bridge measuring ammonia Long-dong, BOD5 measure in that station on a downward trend, DO, and SS is on the rise, River Pollution Index (RPI) on a downward trend. The results form Chau-Jhou station also ahowed simialar trends .more and more near the upstrean Hing-she station raise vivestok Sing-She stations are that ammonia on a upward trend, BOD5 no significant change in trend, DO, and SS is on the rise, river pollution index (RPI) a slight downward trend. Dong-gang River Basin , but the progress of sewer construction in slow. To reduce pollation in this river effort shoul be made regulatory reform on livestock waste control and acceleration of sewer construction. Keywords: quantile regression analysis, BOD5, RPI

78 FR 77467 - Findings of Research Misconduct

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-23

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Office of the Secretary Findings of Research Misconduct... Research Integrity (ORI) has taken final action in the following case: Dong-Pyou Han, Ph.D., Iowa State... conducted by ORI, ORI and ISU found that Dr. Dong-Pyou Han, former Research Assistant Professor, Department...

A Systematic Review of the Mysterious Caterpillar Fungus Ophiocordyceps sinensis in Dong-ChongXiaCao (冬蟲夏草 Dōng Chóng Xià Cǎo) and Related Bioactive Ingredients

PubMed Central

Lo, Hui-Chen; Hsieh, Chienyan; Lin, Fang-Yi; Hsu, Tai-Hao

2013-01-01

The caterpillar fungus Ophiocordyceps sinensis (syn.† Cordyceps sinensis), which was originally used in traditional Tibetan and Chinese medicine, is called either “yartsa gunbu” or “DongChongXiaCao (冬蟲夏草 Dōng Chóng Xià Cǎo)” (“winter worm-summer grass”), respectively. The extremely high price of DongChongXiaCao, approximately USD $20,000 to 40,000 per kg, has led to it being regarded as “soft gold” in China. The multi-fungi hypothesis has been proposed for DongChongXiaCao; however, Hirsutella sinensis is the anamorph of O. sinensis. In Chinese, the meaning of “DongChongXiaCao” is different for O. sinensis, Cordyceps spp.,‡ and Cordyceps spƒ. Over 30 bioactivities, such as immunomodulatory, antitumor, anti-inflammatory, and antioxidant activities, have been reported for wild DongChongXiaCao and for the mycelia and culture supernatants of O. sinensis. These bioactivities derive from over 20 bioactive ingredients, mainly extracellular polysaccharides, intracellular polysaccharides, cordycepin, adenosine, mannitol, and sterols. Other bioactive components have been found as well, including two peptides (cordymin and myriocin), melanin, lovastatin, γ-aminobutyric acid, and cordysinins. Recently, the bioactivities of O. sinensis were described, and they include antiarteriosclerosis, antidepression, and antiosteoporosis activities, photoprotection, prevention and treatment of bowel injury, promotion of endurance capacity, and learning-memory improvement. H. sinensis has the ability to accelerate leukocyte recovery, stimulate lymphocyte proliferation, antidiabetes, and improve kidney injury. Starting January 1st, 2013, regulation will dictate that one fungus can only have one name, which will end the system of using separate names for anamorphs. The anamorph name “H. sinensis” has changed by the International Code of Nomenclature for algae, fungi, and plants to O. sinensis. PMID:24716152

Deep Subwavelength Optical Nanolithography

DTIC Science & Technology

2005-05-12

1295 -1299 (2004). Deying Xia, Dong Li... DAC output signal from the motion profile rather than the position error. Fig 3 The structure of fuzzy logic based adaptive feedforward PI...rewritten as ( )                     + =           ′ ′ c s s c cs I I I TC CT CJC S S S 0 22, ,1 0,00, 2 0 0 0 1 ω ω

Supplementary descriptive notes of the Sinella and Coecobrya (Collembola: Entomobryidae) species from North America, Hawaii and Japan.

PubMed

Wu, Jun; Yan, Qibao; Zhang, Feng

2016-03-04

Many known collembolan species lack sufficient descriptive details so that further taxonomical comparison cannot be achieved. When sorting documents and materials from Prof. Jian-Xiu CHEN, Nanjing University, we found many old but valuable drafts on Sinella and Coecobrya species from North America, Hawaii and Japan. Supplementary descriptions, particularly chaetotaxy, were provided for the following species: S. agna Christiansen & Bellinger, 1980, S. alata Christiansen, 1960, S. baca Christiansen & Bellinger, 1980, S. barri Christiansen, 1960, S. hoffmani Wray, 1952, S. sexoculata (Schött, 1896), S. straminea (Folsom, 1899), S. yosiia Christiansen & Bellinger, 1992, C. akiyoshiana Yosii, 1956, C. borerae Christiansen & Bellinger, 1992, C. ishikawai Yosii, 1956, C. kukae Christiansen & Bellinger, 1992, C. lua Christiansen & Bellinger, 1992, C. nupa Christiansen & Bellinger, 1992, C. tibiotarsalis Yosii, 1964. Sinella cavernarum (Packard, 1888) was redescribed based on topotypes and more additional materials.

Synthetic Decapeptide Enhances Bacterial Clearance and Accelerates Healing in the Wounds of Restraint-Stressed Mice

DTIC Science & Technology

2012-02-06

resistance in cancer . Adv. Drug Deliv. Rev. 54, 759. Kabanov, A.V., Nazarova, I.R., Astafieva, I.V., Batrakova, E.V., Alakhov, Yu, V., Yaroslavov, A.A...S186–S187. Wang, C., Li, M., Dong, D., Wang, J., Ren, J., Otto, M., Gao, Q., 2007. Role of ClpP in biofilm formation and virulence of Staphylococcus

Controle orbital de satélites artificiais com propulsão e uso de gravidade lunar

NASA Astrophysics Data System (ADS)

Torres, K. S.; de Almeida Prado, A. F. B.

2003-08-01

A redução do custo de combustível de uma manobra é atualmente a grande prioridade de todos os programas espaciais existentes no mundo. As manobras assistidas pela gravidade são uma ótima forma de se contornar o problema pois proporcionam economias com vasto impacto no custo final da missão. Neste trabalho é feito um estudo particular do controle orbital de um satélite artificial da Terra usando a gravidade da Lua. O objetivo é estudar uma técnica econômica para uma mudança de plano de um satélite que está em órbita em volta da Terra. A idéia principal desta abordagem é enviar primeiramente o veículo espacial em direção à Lua usando uma manobra mono-impulsiva para que assim o campo gravitacional da Lua possa fazer a mudança de plano desejada (sem custo de combustível) e só então retornar o veículo aos valores iniciais de semi-eixo e excentricidade usando uma manobra bi-impulsiva tipo Hohmann. Para tanto, é assumido que a espaçonave inicia em uma órbita circular coplanar à órbita da lua em torno da Terra e a meta é colocá-la em uma órbita similar que difere da órbita inicial somente pela inclinação. São usadas equações analíticas baseadas na abordagem Patched Conics para se calcular a variação na velocidade, momento angular, energia e inclinação do veículo espacial que realiza esta manobra. Várias simulações são feitas para se avaliar as economias de combustível envolvidas.

A Introdução de Astronomia Básica para Estudantes de 5 e 6 Séries do Ensino Fundamental

NASA Astrophysics Data System (ADS)

Gonzaga, E. P.; Voelzke, M. R.

2008-09-01

Com intuito de despertar o interesse pelo estudo da astronomia, desenvolveu-se entre 2003 e 2006 no Centro Educacional do Serviço Social da Indústria situado no município de Mauá, São Paulo, um trabalho com duração de 12 meses para turmas de 11 e 12 anos, sendo três turmas por período (2003 / 2004, 2004 / 2005 e 2005 / 2006). O trabalho realizado, objetivando a introdução da astronomia básica com observações de constelações e das fases da Lua, permitindo estender-se a outros corpos celestes, iniciou-se com visita ao planetário Mundo Estelar, localizado no Ipiranga, São Paulo levantou-se a questão da importância do estudo da astronomia e foi lançado aos estudantes um trabalho de observação, com a proposta de localizar as constelações e compreender as fases da Lua, para o registro de tais observações, foram distribuídos mapas celestes, pastas, planilhas de anotações com lacunas para, constelações, fases da Lua e relatos, onde os estudantes acrescentaram pesquisas e relatórios. Os resultados dos 346 estudantes foram que 86,7% (300) concluíram a etapa de observação, destes 300 estudantes 43,3% (130) pesquisaram sobre as constelações e 19,0% (57) localizaram até quatro constelações. Dos 346 estudantes, 44,2% (153) registraram as fases da Lua equivocadamente e 10,4% (36) anexaram reportagens sobre astronomia. Conclui-se que os estudantes se dividiram em pesquisadores, sendo os estudantes que registraram e anexaram informações e, os observadores que preencheram as planilhas de observações, além de desenvolverem habilidades e competências relacionadas ao estudo da astronomia.

Astronomy at School: Measurements of the Earth-Moon Distance. (Spanish Title: Astronomía en la Escuela: Medición de la Distancia Tierra-Luna.) Astronomia na Escola: Medida da Distância Terra-Lua

NASA Astrophysics Data System (ADS)

Paolantonio, Santiago; Pintado, Olga I.

2006-12-01

The aim of this project is to approach High School students to Science. We propose to measure the Earth-Moon distance using the parallax. The observation and measurements techniques and all the calculations needed are described in this paper. The results obtained in a test experience realized during 2003 are presented. This test shows that this is a feasible project and also shows which things needed to be improved. Se plantea la propuesta de un proyecto educativo dirigido al Nivel Medio de enseñanza, consistente en la determinación de la distancia Tierra - Luna por paralaje. Se describen las técnicas de observación, mediciones y cálculos. Se proporcionan a continuación los resultados obtenidos en la experiencia piloto realizada en el 2003, la que permitió comprobar la factibilidad de llevar a cabo la propuesta, así como perfeccionar los diferentes aspectos involucrados en la misma. Propomos neste trabalho um projeto educativo dirigido ao Ensino Médio que consiste na determinação da distância Terra-Lua pelo efeito da paralaxe. As técnicas de observação, medidas e cálculos auxiliares são descritos, e os resultados obtidos numa experiência-teste realizada no ano de 2003 apresentados. Este último teste permitiu comprovar a viabilidade de execução da proposta e aperfeiçoar diversos aspectos da mesma.

Narrated Landscape as Counterweight to Perception of Placelessness in Contemporary Urban Landscape: Re-Visioning Place in Gwangbok-Dong and Nampo-Dong, Busan, South Korea

ERIC Educational Resources Information Center

Lee, Sungkyung

2009-01-01

Although the contemporary commercial urban landscape is often assessed as placeless, this research proposes that even these seemingly anonymous places are repositories of thriving community values and meanings. Seeking a more complex reading, this research extends the scope of analysis from physical space to human use in order to reveal the…

Ethnobotany of dye plants in Dong communities of China

PubMed Central

2014-01-01

Background Dyes derived from plants have an extensive history of use for coloring food and clothing in Dong communities and other indigenous areas in the uplands of China. In addition to use as coloring agents, Dong communities have historically utilized dye plants for their value for enhancing the nutritive, medicinal and preservative properties of foods. However, the persistence of plant-derived dyes and associated cultural practices and traditional knowledge is threatened with rapid socio-economic change in China. Research is needed to document the ethnobotany of dye plants in indigenous communities towards their conservation and potential commercialization as a sustainable means of supporting local development initiatives. Methods Semi-structured surveys on plants used for coloring agents and associated traditional knowledge were conducted in fifteen Dong villages of Tongdao County in Hunan Province of South Central China during 2011–2012. Transect walks were carried out with key informants identified from semi-structured surveys to collect samples and voucher specimens for each documented plant species for taxonomic identification. Results Dong households at the study sites utilize the flowers, bark, stems, tubers and roots of 13 plant species from 9 families as dyes to color their customary clothing and food. Out of the documented plants, a total of 7 are used for coloring food, 3 for coloring clothing and 3 for both food and clothing. Documented plants consist of 3 species that yield black pigments, 3 for brownish red/russet pigments, 3 for red pigments, 2 for dark blue pigments and 2 for yellow pigments. In addition to dyes, the plants have multiple uses including medicinal, ornamental, sacrificial, edible, and for timber. Conclusions The use of dyes derived from plants persists at the study sites for their important role in expressing Dong cultural identity through customary clothing and food. Further research is needed to evaluate the safety of dye plants, their efficacy in enhancing food items and their commercial potential. Conservation policies and management plans are called for to preserve these ethnobotanical resources in a sustainable manner that supports local livelihoods while maintaining cultural practices. PMID:24552267

Ethnobotany of dye plants in Dong communities of China.

PubMed

Liu, Yujing; Ahmed, Selena; Liu, Bo; Guo, Zhiyong; Huang, Weijuan; Wu, Xianjin; Li, Shenghua; Zhou, Jiangju; Lei, Qiyi; Long, Chunlin

2014-02-19

Dyes derived from plants have an extensive history of use for coloring food and clothing in Dong communities and other indigenous areas in the uplands of China. In addition to use as coloring agents, Dong communities have historically utilized dye plants for their value for enhancing the nutritive, medicinal and preservative properties of foods. However, the persistence of plant-derived dyes and associated cultural practices and traditional knowledge is threatened with rapid socio-economic change in China. Research is needed to document the ethnobotany of dye plants in indigenous communities towards their conservation and potential commercialization as a sustainable means of supporting local development initiatives. Semi-structured surveys on plants used for coloring agents and associated traditional knowledge were conducted in fifteen Dong villages of Tongdao County in Hunan Province of South Central China during 2011-2012. Transect walks were carried out with key informants identified from semi-structured surveys to collect samples and voucher specimens for each documented plant species for taxonomic identification. Dong households at the study sites utilize the flowers, bark, stems, tubers and roots of 13 plant species from 9 families as dyes to color their customary clothing and food. Out of the documented plants, a total of 7 are used for coloring food, 3 for coloring clothing and 3 for both food and clothing. Documented plants consist of 3 species that yield black pigments, 3 for brownish red/russet pigments, 3 for red pigments, 2 for dark blue pigments and 2 for yellow pigments. In addition to dyes, the plants have multiple uses including medicinal, ornamental, sacrificial, edible, and for timber. The use of dyes derived from plants persists at the study sites for their important role in expressing Dong cultural identity through customary clothing and food. Further research is needed to evaluate the safety of dye plants, their efficacy in enhancing food items and their commercial potential. Conservation policies and management plans are called for to preserve these ethnobotanical resources in a sustainable manner that supports local livelihoods while maintaining cultural practices.

Source identification and mass balance studies of mercury in Lake An-dong, S. Korea

NASA Astrophysics Data System (ADS)

Han, J.; Byeon, M.; Yoon, J.; Park, J.; Lee, M.; Huh, I.; Na, E.; Chung, D.; Shin, S.; Kim, Y.

2009-12-01

In this study, mercury and methylmercury were measured in atmospheric, tributary, open-lake water column, sediment, planktons and fish samples in the catchments area of Lake An-dong, S. Korea. Lake An-dong, an artificial freshwater lake is located on the upstream of River Nak-dong. It has 51.5 km2 of open surface water and 1.33 year of hydraulic residence time. It is a source of drinking water for 0.3 million S. Koreans. Recently, the possibilities of its mercury contamination became an issue since current studies showed that the lake had much higher mercury level in sediment and certain freshwater fish species than any other lakes in S. Korea. This catchments area has the possibilities of historical mercury pollution by the location of more than 50 abandoned gold mines and Young-poong zinc smelter. The objective of this study was to develop a mercury mass balance and identify possible mercury sources in the lake. The results of this study are thus expected to offer valuable insights for the sources of mercury loading through the watershed. In order to estimate the mercury flux, TGM, RGM and particulate mercury were measured using TEKRAN 2537 at the five sites surrounding Lake An-dong from May, 2009 with wet and dry deposition. The fate and transport of mercury in water body were predicted by using EFDC (Environmental Dynamic Fluid Code) and Mercury module in WASP7 (Water quality analysis program) after subsequent distribution into water body, sediments, followed by bioaccumulation and ultimate uptake by humans. The mercury mass balance in Young-poong zinc smelter was also pre-estimated by measuring mercury content in zinc ores, emission gases, sludge, wastewater and products.

U. S. Naval Forces, Vietnam Monthly Historical Supplement for June 1968

DTIC Science & Technology

1969-02-18

some of the cargo, and a Harbor Clearance Unit to assist in rigging beach gear CQk MT1IAL 90 7777 77- CO0N I P waWi usL on the John C. As the tugs which...personnel in Vietnam, a mooring buoy was moved from Ben Tre to Dong Tau. !LLC-l, with Harbor Clearance Team THREE embarked, soiled from Vung Tan on 27 June

Growth and characterization of single crystal rocksalt LaAs using LuAs barrier layers

NASA Astrophysics Data System (ADS)

Krivoy, E. M.; Rahimi, S.; Nair, H. P.; Salas, R.; Maddox, S. J.; Ironside, D. J.; Jiang, Y.; Dasika, V. D.; Ferrer, D. A.; Kelp, G.; Shvets, G.; Akinwande, D.; Bank, S. R.

2012-11-01

We demonstrate the growth of high-quality, single crystal, rocksalt LaAs on III-V substrates; employing thin well-behaved LuAs barriers layers at the III-V/LaAs interfaces to suppress nucleation of other LaAs phases, interfacial reactions between GaAs and LaAs, and polycrystalline LaAs growth. This method enables growth of single crystal epitaxial rocksalt LaAs with enhanced structural and electrical properties. Temperature-dependent resistivity and optical reflectivity measurements suggest that epitaxial LaAs is semimetallic, consistent with bandstructure calculations in literature. LaAs exhibits distinct electrical and optical properties, as compared with previously reported rare-earth arsenide materials, with a room-temperature resistivity of ˜459 μΩ-cm and an optical transmission window >50% between ˜3-5 μm.

Quantum Phases of Matter in Optical Lattices

DTIC Science & Technology

2015-06-30

doi: 10.1103/PhysRevA.89.013625 Hyungwon Kim, David A. Huse. Ballistic Spreading of Entanglement in a Diffusive Nonintegrable System, Physical...Review B, (07 2013): 0. doi: 10.1103/PhysRevB.88.014206 Lin Dong, Lei Jiang, Han Pu. Fulde–Ferrell pairing instability in spin–orbit coupled Fermi...PhysRevA.87.051603 Kuei Sun, C. J. Bolech. Pair tunneling, phase separation, and dimensional crossover in imbalanced fermionic superfluids in a coupled

Amylase and Lipase Detection in Hemorrhaged Animals Treated with HBOC-201

DTIC Science & Technology

2011-01-01

Biopure Corp., Cambridge, MA, and Abbott Laboratories, Chicago, n.., respectively. We are thankful to Yao Yang and Mike Hammett for technical...amylase readings above baseline. Regression Equations : Amyl (%) = 1001J.0899 * HBOC-201 concentration (r = 0.993) for serum; Amyl (%) = 1000·0sss... Hammett , M., Asher, L., Philbin, N., Rice, J., Dong, F., Pearce, B., Flournoy, W., Nicholson, C., McCarron, R., and Freilich, D. (2005). Effects of bovine

Aeronautical Materials (Selected Articles),

DTIC Science & Technology

1984-03-13

directional technique. We would like to deeply thank comrades Dong Yuzhuo, Liu Nianqing, Yuan Pinzhen, Sun Shuzhen, Li Shuhuan etc. and other fraternal units...1Burton, C.J7., "Superalloys, Metallurgy and -Manufacture", Li 1976, p14 7. P [3 1Cai Julin and Zheng Yunrong, "A Study of the Interdend- ritic...of the Beijing Institute of Aeronautical Materials, Liu Ansheng and Li Yonghong of the General Academy of Nonferrous Metals, Yang Yanhua of the Coal

Physical Processes in Hollow Cathode Discharge

DTIC Science & Technology

1989-12-01

State University. Finally, many thanks to my wife, Kyoung -Sook and my son, Frederick Teut, for their love and being supportive for two and half years...recommended for all electron emission purposes. 46 REFERENCES 1. Kim Gunther, "Hollow Cathode Plasma Source" ( Spectra-Mat Hollow Cathode Manual...59 Dong 401 Ho Seoul, Republic of Korea 8. Maj. Kim , Jong-Ryul 1 Postal Code 500-00 Book-Gu, Du-Am Dong, 874-14 Kwang-Ju, Republic of Korea 9. Maj

Development and Translation of a Tissue-Engineered Disc in a Preclinical Rodent Model

DTIC Science & Technology

2013-10-01

authors Dong Hwa Kim, John T. Martin, Dawn M. Elliott, Lachlan J. Smith , and Robert L. Mauck...Replacement in a Small Animal Model with authors John T. Martin, Andrew H. Milby, Joseph A. Chiaro, Dong Hwa Kim, Nader M. Hebela, Lachlan J. Smith , Dawn... Smith , Dawn M. Elliott, and Robert L. Mauck is now in review. To carry out this study, radiopaque scaffolds were generated from a 14.3% w/v slurry

Blood Group Antigens on HeLa Cells shown by Mixed Agglutination

PubMed Central

Kelus, A.; Gurner, B. W.; Coombs, R. R. A.

1959-01-01

The mixed agglutination reaction has been used for investigating the presence of blood group antigens on the surface of human cervical carcinoma cells (HeLa) cultured for eight years in vitro. The H antigen was demonstrated in the absence of A and B. The MN-type antigen has been found as well as Tja. Treatment of HeLa cells with ficin greatly enhanced the reaction of anti-H and anti-Tja with the corresponding antigens on HeLa cells. The authors failed to show the following antigens: Rh(D) and Rh(c), S, P, Lea, Leb, Lua and Lub. ImagesFIG. 1FIG. 2 PMID:14405338

Temporal and spatial analysis of vegetation coverage changes in Ordos area based on time series GIMMS-NDVI data

NASA Astrophysics Data System (ADS)

Han, Ruimei; Zou, Youfeng; Ma, Chao; Liu, Pei

2014-11-01

Ordos area is the desert-wind erosion desertification steppe transition zone and the complex ecological zone. As the research area, Ordos City has the similar natural geographic environment to ShenDong coalfield. To research its ecological patterns and natural evolution law, it has instructive to reveal temporal and spatial changes of ecological environment with artificial disturbance in western mining. In this paper, a time series of AVHRR-NDVI(Normalized Difference Vegetation Index) data was used to monitor the change of vegetation temporal and spatial dynamics from 1981 to 2006 in Ordos City and ShenDong coalfield, where were as the research area. The MVC (Maximum Value Composites) method, average operation, linear regression, and gradation for NDVI change trend were used to obtained some results, as follows: ¬vegetation coverage had obvious characteristics with periodic change in research area for 26 years, and vegetation growth peak appeared on August, while the lowest appeared on January. The extreme values in Ordos City were 0.2351 and 0.1176, while they were 0.2657 and 0.1272 in ShenDong coalfield. The NDVI value fluctuation was a modest rise trend overall in research area. The extreme values were 0.3071 and 0.1861 in Ordos City, while they were 0.3454 and 0.1904 in ShenDong coalfield. In spatial distribution, slight improvement area and slight degradation area were accounting for 42.49% and 8.37% in Ordos City, while slight improvement area moderate improvement area were accounting for 70.59% and 29.41% in ShenDong coalfield. Above of results indicated there was less vegetation coverage in research area, which reflected the characteristics of fragile natural geographical environment. In addition, vegetation coverage was with a modest rise on the whole, which reflected the natural environment change.

Superfluid turbulence in a nonuniform circular channel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Murphy, P.J.

The excess dissipation due to the presence of quantized vorticity in flowing helium has been studied extensively. The success of the microscopic theory proposed by Schwarz in describing many properties of this dissipation led to a belief that the major aspects of the problem had been understood at the microscopic level. The experiment of Kafkalidis and Tough demonstrated that a weak one dimensional nonuniformity in the flow field led to a dramatic departure between the observed behavior and the predictions of the Schwarz theory using the local uniformity approximation (LUA). The research presented in this thesis was undertaken to measuremore » the dissipative states for thermal counterflow with a weak two dimensional nonuniformity. The experiment of Kafkalidis and Tough used a flow channel with a high aspect ratio. Such channels are known to exhibit only one state of superfluid turbulence. In this research the channel is circular in cross section and shows two distinct turbulent states (T-I and T-II). This experiment demonstrates that there is no difference in the excess dissipation for flows that are either converging or diverging. The T-I state is described by the same parameters as the T-I state in uniform channels. The turbulence exhibits front behavior at the transition between states. These conclusions are consistent with the LUA. The T-II state is at variance with the LUA, but is consistent with the results found in the Kafkalidis and Tough experiment.« less

Study of the Physics of Insulating Films as Related to the Reliability of Metal-Oxide Semiconductor Devices

DTIC Science & Technology

1980-11-01

materials work and sample preparation of D.W. Dong; the technical assistance of F.L. Pesavento and J.A. Calise; the assistance in device fabrication...FILMS D.J. DiMaria R. Ghez D.W. Dong I.B.M. Thomas J. Watson Research Center Yorktown Heights, New York 10598 Technical Assistance of F.L. Pesavento and... Pesavento and J.A. Calise; the assistance with gate metallizations by the Silicon Facility and Central Scientific Services at the T.J. Watson

Magnetic Properties of the DNA-Quaternary Ammonium Surfactant Complexes Studied by EMR Spectroscopy and SQUID Measurement

DTIC Science & Technology

2008-09-24

Eui Doo Do, Dong Hoon Choi, and Jung -Il Jin∗ Department of Chemistry, Korea University, Seoul 136-701, Korea Chang Hoon Lee Department of Polymer...Electromagnetic Properties of Modified DNA 5a. CONTRACT NUMBER FA48690714023 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Jung -Il Jin 5d...the last temperature- independent χPauli is the Pauli spin susceptibility attributed by delocalized con- ductive spin species. The molar

University Students' Conceptions about the Moon Phases. (Spanish Title: Concepciones de Estudiantes Universitários sobre Las Fases de la Luna.) Concepções de Estudantes Universitários sobre as Fases da Lua

NASA Astrophysics Data System (ADS)

de Fátima Oliveira Saraiva, Maria; da Silveira, Fernando Lang; Steffani, Maria Helena

2011-07-01

In this article we describe the development of a multiple choice test about lunar phases and analyze the results of its application to ten groups of Physics students at the UFRGS. During the improvement of the test, we noticed that the percentage of right answers about some concepts increased significantly when associated with the reformulation of the question, emphasizing the importance of being careful to avoid incorrect answers generated by unclear questions, and not by ignorance on the matter. We confirm the results of other studies that show that students have great difficulty to relate the Moon's phase with its position in the sky at given time. On the other hand, our results suggest that, in general, students of Physics understand the phenomenon of lunar phases better than the average of university students. En estese artículo se describe la elaboración de una prueba de opción múltiple sobre las fases de la Luna y se analizan los resultados de su aplicación en diez grupos de estudiantes de Física de UFRGS. Durante el mejoramiento de la prueba observamos que el porcentaje de aciertos creció considerablemente cuando considerada una nueva redacción de la pregunta, destacando el cuidado que se debe tomar a fin de evitar respuestas incorrectas generadas por preguntas poco claras y no a causa de la ignorancia de los estudiantes sobre el tema. Confirmamos los resultados de otros estudios que las mayores dificultades de los alumnos sobre el tema fases de la Luna están en relacionar la fase de la Luna con su posición en el cielo en determinado momento. Por otra parte, nuestros resultados sugieren que, en general, los estudiantes de la Física comprenden mejor el fenómeno de las fases lunares que el promedio de los estudiantes universitarios. Neste artigo descrevemos a elaboração de um teste de múltipla escolha sobre as fases da Lua e analisamos os resultados de sua aplicação em dez grupos de estudantes de Física da UFRGS. Durante o aprimoramento do teste notamos que a porcentagem de acertos a respeito de alguns conceitos teve um aumento significativo associado à reformulação da pergunta, ressaltando a importância de tomar cuidado para evitar respostas erradas geradas por perguntas pouco claras e não por ignorância do respondente sobre o assunto. Confirmamos os resultados de outros estudos de que as maiores dificuldades dos alunos sobre o tema Fases da Lua dizem respeito a relacionar a fase que a Lua apresenta com a sua posição no céu em determinada hora. Por outro lado, nossos resultados sugerem que, em geral, os estudantes de Física entendem melhor o fenômeno das fases lunares do que a média dos estudantes universitários.

What Next After Ho Chi Minh?

DTIC Science & Technology

1966-04-08

Propagands Department; Nguyen Chi Thanh, protege of Truong Chinh ; Nguyen Duy Trinh; and Le Duc Thoo The pro-Soviet faction consists of Pham Van Dong...Communist" "Pro-Soviet" Ho Chi Minh Le Duan Truong Chinh Pham Van Dong Pham Hung General Giap Le Duc Tho Nguyen Chi Thanh Nguyen Duy Trinh Le Thanh Nghi...0 o . 15 The contenders. 0 . . . .0.0 28 4a THE LINE-UP 0 * a 0 * * 0 0 31 The lessor three - Hoang Van Hoan, Le Duc Tho, Le Thanh Nghi o .* 0 * * 0

Recent advances of mid-infrared compact, field deployable sensors: principles and applications

NASA Astrophysics Data System (ADS)

Tittel, Frank; Gluszek, Aleksander; Hudzikowski, Arkadiusz; Dong, Lei; Li, Chunguang; Patimisco, Pietro; Sampaolo, Angelo; Spagnolo, Vincenzo; Wojtas, Jacek

2016-04-01

The recent development of compact interband cascade lasers(ICLs) and quantum cascade lasers (QCLs) based trace gas sensors will permit the targeting of strong fundamental rotational-vibrational transitions in the mid-infrared which are one to two orders of magnitude more intense than transitions in the overtone and combination bands in the near-infrared. This has led to the design and fabrication of mid-infrared compact, field deployable sensors for use in the petrochemical industry, environmental monitoring and atmospheric chemistry. Specifically, the spectroscopic detection and monitoring of four molecular species, methane (CH4) [1], ethane (C2H6), formaldehyde (H2CO) [2] and hydrogen sulphide (H2S) [3] will be described. CH4, C2H6 and H2CO can be detected using two detection techniques: mid-infrared tunable laser absorption spectroscopy (TDLAS) using a compact multi-pass gas cell and quartz enhanced photoacoustic spectroscopy (QEPAS). Both techniques utilize state-of-the-art mid-IR, continuous wave (CW), distributed feedback (DFB) ICLs and QCLs. TDLAS was performed with an ultra-compact 54.6m effective optical path length innovative spherical multipass gas cell capable of 435 passes between two concave mirrors separated by 12.5 cm. QEPAS used a small robust absorption detection module (ADM) which consists of a quartz tuning fork (QTF), two optical windows, gas inlet/outlet ports and a low noise frequency pre-amplifier. Wavelength modulation and second harmonic detection were employed for spectral data processing. TDLAS and QEPAS can achieve minimum detectable absorption losses in the range from 10-8 to 10-11cm-1/Hz1/2. Several recent examples of real world applications of field deployable gas sensors will be described. For example, an ICL based TDLAS sensor system is capable of detecting CH4 and C2H6 concentration levels of 1 ppb in a 1 sec. sampling time, using an ultra-compact, robust sensor architecture. H2S detection was realized with a THz QEPAS sensor system using a custom quartz tuning fork (QTF) with a new geometry and a QCL emitting at 2.913 THz [4]. Furthermore, two new approaches aimed to achieve enhanced detection sensitivities with QEPAS based sensing can be realized. The first method will make use of a compact optical power buildup cavity, which achieves significantly lower minimum detectable trace gas concentration levels of < 10 pptv. The second approach will use custom fabricated QTFs capable of improved detection sensitivity. Acknowledgements F.K. Tittel acknowledges support by the National Science Foundation (NSF) ERC MIRTHE award, the Robert Welch Foundation (Grant C-0586) and DOE ARPA-E Monitor Proram. L. Dong acknowledges support by NSF-China (Grant #s. 61275213, 61108030), J. Wojtas acknowledges support by The National Centre for Research and Development, Poland (project ID: 179616). References [1] L. Dong, C. Li, N. P. Sanchez, A. K. Gluszek, R. Griffin and F. K. Tittel;" Compact CH4 sensor system based on a continuous-wave, low power consumption, room temperature interband cascade laser", Appl. Phys Lett. 108, 011106 (2016). [2] L. Dong, Y. Yu, C. Li, S. So, and F.K. Tittel, "Ppb-level formaldehyde detection using a CW room-temperature interband cascade laser and a miniature dense pattern multipass cell" Optics Express; 23, 19821-19830 (2015). [3] V. Spagnolo, P. Patimisco, R. Pennetta, A. Sampaolo, G. Scamarcio, M. Vitiello, and F.K. Tittel, "THz Quartz-enhanced photoacoustic sensor for H2S trace gas detection", Opt. Exp. 23, 7574-7582 (2015). [4] A. Sampaolo, P. Patimisco, L. Dong , A. Geras, S, G. Scamarcio' T. Starecki, F.K Tittel, V. Spagnolo; "Quartz-Enhanced Photoacoustic Spectroscopy exploiting tuning fork overtone modes", Appl. Phys Lett. 107, 231102 (2015).

Photoluminescent (PL) or electroluminescent (EL) quantum dots for display, lighting, and photomedicine (Conference Presentation)

NASA Astrophysics Data System (ADS)

Dong, Yajie

2017-02-01

Quantum dots (QDs) have gone through a long journey before finding their ways into the display field. This talk will briefly touch on the history before trying to answer several key questions related to QDs applications in display: What are QDs? How are they made? What properties do they have and Why? How can these properties be used to improve color and efficiency of display, in either photoluminescence (PL) or electroluminescence (EL) mode? And what are the remaining challenges for QDs wide adoption in display industry? Lastly, some most recent progresses in our UCF lab at both PL and EL fronts will be highlighted. For PL, a cadmium-free perovskite-polymer composite films with exceptionally narrow emission green peaks (FWHM 20 nm) and good water and thermal stability will be reported. Together with red quantum dots or PFS/KSF phosphors as down-converters for blue LEDs, a white-light source with 95% Rec. 2020 color gamut was demonstrated [1]. For EL, red quantum dot light emitting devices (QLEDs) with record luminance of 165,000 Cd/m2 has been obtained at a current density of 1000 mA/cm2 with a low driving voltage of 5.8 V and CIE coordinates of (0.69, 0.31). [2] The potential of using these QLEDs for light sources for integrated sensing platform [3] or high efficiency, high color quality hybrid white OLED [4] will be discussed. [1] Y. N. Wang, J. He, H. Chen, J. S. Chen, R. D. Zhu, P. Ma, A. Towers, Y. Lin, A. J. Gesquiere, S. T. Wu, Y. J. Dong. Ultrastable, Highly Luminescent Organic-Inorganic Perovskite - Polymer Composite Films, Advanced Materials, accepted, (2016). [2] Y. J. Dong, J.M. Caruge, Z. Q. Zhou, C. Hamilton, Z. Popovic, J. Ho, M. Stevenson, G. Liu, V. Bulovic, M. Bawendi, P. T. Kazlas, S. Coe-Sullivan, and J. Steckel Ultra-bright, Highly Efficient, Low Roll-off Inverted Quantum-Dot Light Emitting Devices (QLEDs). SID Symp. Dig. Tech. Pap. 46, 270-273 (2015). [3] J. He, H. Chen, S. T. Wu, and Y. J. Dong, Integrated Sensing Platform Based on Quantum Dot Light Emitting Diodes. SID Symp. Dig. Tech. Pap. 47, 344-346 (2016). [4] H. Chen, J. He, J. S. Chen, S. T. Wu and Y. J. Dong, High Efficacy, High Color Quality Hybrid White OLEDs Incorporating Red Quantum Dots with Narrow Emission Bands. SID Symp. Dig. Tech. Pap. 47, 50-52 (2016).

Extending Mode Areas of Single-mode All-solid Photonic Bandgap Fibers

DTIC Science & Technology

2015-04-02

T. Tunnermann, R. Iliew, F. Lederer, J. Broeng, G. Vienne, A. Petersson, and C. Jakobsen, “High-power air-clad large-mode-area photonic crystal ...Yvernault, and F. Salin, “Extended single-mode photonic crystal fiber lasers,” Opt. Express 14(7), 2715–2720 (2006). 10. L. Dong, T. Wu, H. McKay, L. Fu...progress in mode area scaling of optical fibers. One notable area is in photonic crystal fibers (PCF) [3–5, 8, 9]. The short straight PCF rods used in

Design and Flood Control Assessment of 5MWp Fishing and Photovoltaic Power Project in Xinghua City

NASA Astrophysics Data System (ADS)

Guo, Liuchao; Hu, Xiaodong; Su, Yuyan; Wu, Peipei; Weng, Songgan

2017-12-01

In order to reduce coal consumption in Jiangsu Province and develop new energy sources, considering on the distribution of geology, solar energy resources, traffic and grid connection in Xinghua City, the aim is to determine the configuration of photovoltaic modules and photovoltaic array tracking mode, design photovoltaic array and layout scheme. But the project is a wading project, it is built in Dong Tan Lake polder I115, it needs scientific and reasonable evaluation to the effect of Dong Tan Lake’s flood storage and discharge. The results can provide guidance for similar engineering’s design.

Featured Image: Simulating Planetary Gaps

NASA Astrophysics Data System (ADS)

Kohler, Susanna

2017-03-01

The authors model of howthe above disk would look as we observe it in a scattered-light image. The morphology of the gap can be used to estimate the mass of the planet that caused it. [Dong Fung 2017]The above image from a computer simulation reveals the dust structure of a protoplanetary disk (with the star obscured in the center) as a newly formed planet orbits within it. A recent study by Ruobing Dong (Steward Observatory, University of Arizona) and Jeffrey Fung (University of California, Berkeley) examines how we can determine mass of such a planet based on our observations of the gap that the planet opens in the disk as it orbits. The authors models help us to better understand how our observations of gaps might change if the disk is inclined relative to our line of sight, and how we can still constrain the mass of the gap-opening planet and the viscosity of the disk from the scattered-light images we have recently begun to obtain of distant protoplanetary disks. For more information, check out the paper below!CitationRuobing Dong () and Jeffrey Fung () 2017 ApJ 835 146. doi:10.3847/1538-4357/835/2/146

Comparação de modelos para o cálculo de perturbações orbitais devidas à maré terrestre

NASA Astrophysics Data System (ADS)

Vieira Pinto, J.; Vilhena de Moraes, R.

2003-08-01

Aplicações recentes de satélites artificiais com finalidades geodinâmicas requerem órbitas determinadas com bastante precisão. Em particular marés terrestres influenciam o potencial terrestre causando perturbações adicionais no movimento de satélites artificiais, as quais tem sido medidas por diversos processos. A atração exercida pela lua e pelo sol sobre a terra produz deslocamentos elásticos em seu interior e uma protuberância em sua superfície. O resultado é uma pequena variação na distribuição da massa na terra, consequentemente no geopotencial. As perturbações nos elementos orbitais de satélites artificiais terrestres devidas a maré terrestre podem ser estudadas a partir das equações de Lagrange, considerando-se um conveniente potencial. Por outro lado, como tem sido feito pelo IERS, as mudanças induzidas pela maré terrestre no geopotencial podem ser convenientemente modeladas como variações nos coeficientes Cnm e Snm do geopotencial. As duas teorias ainda não foram comparados para um mesmo satélite. Neste trabalho são apresentadas e comparadas as variações de longo período e seculares nas perturbações orbitais devidas à maré terrestre, calculadas por um modelo simples, o de Kozai, e pelo modelo do IERS. Resultados preliminares mostram, para os satélites SCD2 e CBERS1, e para a Lua em movimento elíptico e precessionando, as perturbações seculares no argumento do perigeu e na longitude do nodo ascendente.

The Moon Phases in a Paper Box. (Spanish Title: Las Fases de la Luna en Una Caja de Cartón.) As Fases da Lua Numa Caixa de Papelão

NASA Astrophysics Data System (ADS)

de Fátima O. Saraiva, Maria; Amador, Cláudio B.; Kemper, Érico; Goulart, Paulo; Muller, Angela

2007-12-01

We present a very simple concrete model to demonstrate the concept of phases of an illuminated body. The main objective of our model is to help the understanding of the Moon phases as viewed from the perspective of an observer on Earth. The material allows the visualization of two important effects: (1st) even though all the time half Moon is illuminated by the Sun, we see different fractions of the illuminated Moon surface, depending on our angle of sight; (2nd) the orientation of the convex part of the Moon in the crescent and waning phases on the sky also depends on our perspective from Earth. The use of a closed box allows one to see the contrast among the different phases with no need of a dark room. We also present a text on the Moon phases, emphasizing the dependence of the aspect of the bright part on the angle of sight. En este trabajo proponemos la construcción de material didáctico de bajo costo para demostración del concepto de fases de un cuerpo iluminado. El principal objetivo de nuestro material es facilitar la comprensión de las fases de la Luna desde la perspectiva de un observador en la Tierra. El material ayuda la visualización de dos efectos importantes: (1º) a pesar de tener siempre la mitad de la Luna (representada por una bolita de espuma plástica o de ping-pong), iluminada por el Sol ( representado por una fuente de luz natural o artificial), vemos diferentes fracciones de su superficie iluminada, dependiendo del ángulo por el cual la vemos; (2º) la orientación del borde convexo de la Luna en las fases Creciente y Menguante también depende de la perspectiva por la cual la miramos desde la Tierra. El uso de una caja cerrada permite observar el contraste entre las diferentes fases sin necesidad de estar en un recinto oscuro. Presentamos también un texto explicativo sobre las fases de la Luna, enfatizando la dependencia de la apariencia de la parte iluminada con el ángulo de visión. Neste trabalho propomos a construção de material didático de baixo custo para demonstração do conceito de fases de um corpo iluminado. O principal objetivo de nosso material é facilitar a compreensão das fases da Lua da perspectiva de um observador na Terra. O material ajuda na visualização de dois efeitos importantes: (1º) mesmo tendo sempre a metade da "Lua" (representada por uma bolinha de isopor ou de ping-pong) iluminada pelo "Sol" (representado por uma fonte de luz natural ou artificial), nós vemos diferentes frações de sua superfície iluminada, dependendo do ângulo pelo qual a olhamos; (2º) a orientação da borda convexa da Lua nas fases Crescente e Minguante também depende da perspectiva pela qual a olhamos da Terra. O uso de uma caixa fechada permite observar o contraste entre as diferentes fases sem necessidade de estar em uma sala escurecida. Apresentamos também um texto explicativo sobre fases da Lua, enfatizando a dependência da aparência da parte iluminada com o ângulo de visada.

The Meteoritical Bulletin, No. 81, 1997 July

USGS Publications Warehouse

Grossman, J.N.

1997-01-01

Meteoritical Bulletin, No. 81 lists 181 new meteorites. Noteworthy among these are a new lunar meteorite (Dar al Gani 262), four observed falls (Dong Ujimqin Qi, Galkiv, Mount Tazerzait, and Piplia Kalan), four irons (Albion, Great Sand Sea 003, Hot Springs, and Mont Dieu), two mesosiderites (Dong Ujimqin Qi and Lamont), an acapulcoite (FRO 95029), a eucrite (Piplia Kalan), two probably-paired ureilites (Dar al Gani 164 and 165), an R chondrite (Hammadah al Hamra 119), an ungrouped type-3 chondrite (Hammadah al Hamra 180), a highly unequilibrated ordinary chondrite (Wells, LL3.3), and a variety of carbonaceous and unequilibrated ordinary chondrites from Libya and Antarctica. All italicized abbreviations refer to addresses listed in the appendix. ?? Meteoritical Society, 1997.

[Textual research on Guang dong xin yu (New Sayings of Guangdong) quoted in Ben cao gang mu shi yi (Supplements to Compendium of Materia Medica].

PubMed

Zhang, Ruixian; Zhang, Wei; Li, Jian; Liang, Fei

2014-05-01

Altogether 15 terms for Guang dong xin yu (New Sayings of Guangdong) were used in Ben cao gang mu shi yi (Supplements to Compendium of Materia Medica), including Yue yu (Cantonese sayings), Chong yu (Sayings from Insect Drug), Jie yu (Sayings from Crustacean Drug), Xin yu (New Sayings), Yue hai xiang yu (Fragrant Sayings from Cantonese Region), Yue zhi mu yu (Sayings from Plants in Cantonese Annals), Guang dong suo yu (Trivial Sayings from Guangdong), Yue shan lu (Records of Cantonese Mountains), Yue lu (Cantonese Records), Jiao guang lu (Joint Guangdong Records), Yue cao zhi (Records of Cantonese Grasses), Guang guo lu (Records of Guangdong Fruits), Nan yue suo ji (Trivial Records of Southern Canton), Guang zhi (Guangdong Records), Yue zhi (Cantonese Records) etc. dealing with 57 sorts of drugs (with individual overlapping ones), the author of Xin yu was Qu Dajun, a surviving fogy of the Ming Dynasty actively involved in the activities to restore the old dynasty and resist the Qing Dynasty, and was persecuted in the literary inquisition in which his works were burnt so that Zhao Xuemin, when quoting his texts, had to go in a roundabout way.

Assessment of forest degradation in Vietnam using Landsat time series data

USGS Publications Warehouse

Vogelmann, James; Van Khoa, Phung; Xuan Lan, Do; Shermeyer, Jacob S.; Shi, Hua; Wimberly, Michael C.; Tat Duong, Hoang; Van Huong, Le

2017-01-01

Landsat time series data were used to characterize forest degradation in Lam Dong Province, Vietnam. We conducted three types of image change analyses using Landsat time series data to characterize the land cover changes. Our analyses concentrated on the timeframe of 1973–2014, with much emphasis on the latter part of that range. We conducted a field trip through Lam Dong Province to develop a better understanding of the ground conditions of the region, during which we obtained many photographs of representative forest sites with Global Positioning System locations to assist us in our image interpretations. High-resolution Google Earth imagery and Landsat data of the region were used to validate results. In general, our analyses indicated that many land-use changes have occurred throughout Lam Dong Province, including gradual forest to non-forest transitions. Recent changes are most marked along the relatively narrow interfaces between agricultural and forest areas that occur towards the boundaries of the province. One important observation is that the most highly protected national reserves in the region have not changed much over the entire Landsat timeframe (1972–present). Spectral changes within these regions have not occurred at the same levels as those areas adjacent to the reserves. 

Drought Induced Changes in Growth, Osmolyte Accumulation and Antioxidant Metabolism of Three Maize Hybrids

PubMed Central

Anjum, Shakeel A.; Ashraf, Umair; Tanveer, Mohsin; Khan, Imran; Hussain, Saddam; Shahzad, Babar; Zohaib, Ali; Abbas, Farhat; Saleem, Muhammad F.; Ali, Iftikhar; Wang, Long C.

2017-01-01

Consequences of drought stress in crop production systems are perhaps more deleterious than other abiotic stresses under changing climatic scenarios. Regulations of physio-biochemical responses of plants under drought stress can be used as markers for drought stress tolerance in selection and breeding. The present study was conducted to appraise the performance of three different maize hybrids (Dong Dan 80, Wan Dan 13, and Run Nong 35) under well-watered, low, moderate and SD conditions maintained at 100, 80, 60, and 40% of field capacity, respectively. Compared with well-watered conditions, drought stress caused oxidative stress by excessive production of reactive oxygen species (ROS) which led to reduced growth and yield formation in all maize hybrids; nevertheless, negative effects of drought stress were more prominent in Run Nong 35. Drought-induced osmolyte accumulation and strong enzymatic and non-enzymatic defense systems prevented the severe damage in Dong Dan 80. Overall performance of all maize hybrids under drought stress was recorded as: Dong Dan 80 > Wan Dan 13 > Run Nong 35 with 6.39, 7.35, and 16.55% yield reductions. Consequently, these biochemical traits and differential physiological responses might be helpful to develop drought tolerance genotypes that can withstand water-deficit conditions with minimum yield losses. PMID:28220130

U.S. EPA, Pesticide Product Label, SBP-1382/ESOBIOTHRIN/P.B.O INSEC. AEROSOL 0.2% + 0.3% + 1.2% FORMULA, 10/14/1988

EPA Pesticide Factsheets

2011-04-14

... 11,.,1., PlllCAm .. uy "Innall lua_" •• 1 ••• _ •••• IIC 111111'" CAIn I III 0S • .,.. ... II..... T... ..I' ••• 1 .... _ ." , ,I •• ,.bl? a • .... 2 ..... __ ~ ... ...

Data-driven approach to human motion modeling with Lua and gesture description language

NASA Astrophysics Data System (ADS)

Hachaj, Tomasz; Koptyra, Katarzyna; Ogiela, Marek R.

2017-03-01

The aim of this paper is to present the novel proposition of the human motion modelling and recognition approach that enables real time MoCap signal evaluation. By motions (actions) recognition we mean classification. The role of this approach is to propose the syntactic description procedure that can be easily understood, learnt and used in various motion modelling and recognition tasks in all MoCap systems no matter if they are vision or wearable sensor based. To do so we have prepared extension of Gesture Description Language (GDL) methodology that enables movements description and real-time recognition so that it can use not only positional coordinates of body joints but virtually any type of discreetly measured output MoCap signals like accelerometer, magnetometer or gyroscope. We have also prepared and evaluated the cross-platform implementation of this approach using Lua scripting language and JAVA technology. This implementation is called Data Driven GDL (DD-GDL). In tested scenarios the average execution speed is above 100 frames per second which is an acquisition time of many popular MoCap solutions.

Rebuttal to "On the distribution of the modulus of Gabor wavelet coefficients and the upper bound of the dimensionless smoothness index in the case of additive Gaussian noises: Revisited" by Dong Wang, Qiang Zhou, and Kwok-Leung Tsui

NASA Astrophysics Data System (ADS)

Soltani Bozchalooi, Iman; Liang, Ming

2018-04-01

A discussion paper entitled "On the distribution of the modulus of Gabor wavelet coefficients and the upper bound of the dimensionless smoothness index in the case of additive Gaussian noises: revisited" by Dong Wang, Qiang Zhou, Kwok-Leung Tsui has been brought to our attention recently. This discussion paper (hereafter called Wang et al. paper) is based on arguments that are fundamentally incorrect and which we rebut within this commentary. However, as the flaws in the arguments proposed by Wang et al. are clear, we will keep this rebuttal as brief as possible.

Tao Dong | NREL

Science.gov Websites

, catalysis, and biodiesel Education Ph.D., Bio-Systems Engineering, Washington State University, 2008-2013 Using Acidic Catalyst Generated from Pyrolysis-Derived Bio-Char," Energy Conversion Management

Development of the scintillator-based probe for fast-ion losses in the HL-2A tokamak

NASA Astrophysics Data System (ADS)

Zhang, Y. P.; Liu, Yi; Luo, X. B.; Isobe, M.; Yuan, G. L.; Liu, Y. Q.; Hua, Y.; Song, X. Y.; Yang, J. W.; Li, X.; Chen, W.; Li, Y.; Yan, L. W.; Song, X. M.; Yang, Q. W.; Duan, X. R.

2014-05-01

A new scintillator-based lost fast-ion probe (SLIP) has been developed and operated in the HL-2A tokamak [L. W. Yan, X. R. Duan, X. T. Ding, J. Q. Dong, Q. W. Yang, Yi Liu, X. L. Zou, D. Q. Liu, W. M. Xuan, L. Y. Chen, J. Rao, X. M. Song, Y. Huang, W. C. Mao, Q. M. Wang, Q. Li, Z. Cao, B. Li, J. Y. Cao, G. J. Lei, J. H. Zhang, X. D. Li, W. Chen, J. Chen, C. H. Cui, Z. Y. Cui, Z. C. Deng, Y. B. Dong, B. B. Feng, Q. D. Gao, X. Y. Han, W. Y. Hong, M. Huang, X. Q. Ji, Z. H. Kang, D. F. Kong, T. Lan, G. S. Li, H. J. Li, Qing Li, W. Li, Y. G. Li, A. D. Liu, Z. T. Liu, C. W. Luo, X. H. Mao, Y. D. Pan, J. F. Peng, Z. B. Shi, S. D. Song, X. Y. Song, H. J. Sun, A. K. Wang, M. X. Wang, Y. Q. Wang, W. W. Xiao, Y. F. Xie, L. H. Yao, D. L. Yu, B. S. Yuan, K. J. Zhao, G. W. Zhong, J. Zhou, J. C. Yan, C. X. Yu, C. H. Pan, Y. Liu, and the HL-2A Team, Nucl. Fusion 51, 094016 (2011)] to measure the losses of neutral beam ions. The design of the probe is based on the concept of the α-particle detectors on Tokamak Fusion Test Reactor (TFTR) using scintillator plates. The probe is capable of traveling across an equatorial plane port and sweeping the aperture angle rotationally with respect to the axis of the probe shaft by two step motors, in order to optimize the radial position and the collimator angle. The energy and the pitch angle of the lost fast ions can be simultaneously measured if the two-dimensional image of scintillation light intensity due to the impact of the lost fast ions is detected. Measurements of the fast-ion losses using the probe have been performed during HL-2A neutral beam injection discharges. The clear experimental evidence of enhanced losses of beam ions during disruptions has been obtained by means of the SLIP system. A detailed description of the probe system and the first experimental results are reported.

Development of the scintillator-based probe for fast-ion losses in the HL-2A tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Y. P., E-mail: zhangyp@swip.ac.cn; Liu, Yi; Yuan, G. L.

A new scintillator-based lost fast-ion probe (SLIP) has been developed and operated in the HL-2A tokamak [L. W. Yan, X. R. Duan, X. T. Ding, J. Q. Dong, Q. W. Yang, Yi Liu, X. L. Zou, D. Q. Liu, W. M. Xuan, L. Y. Chen, J. Rao, X. M. Song, Y. Huang, W. C. Mao, Q. M. Wang, Q. Li, Z. Cao, B. Li, J. Y. Cao, G. J. Lei, J. H. Zhang, X. D. Li, W. Chen, J. Chen, C. H. Cui, Z. Y. Cui, Z. C. Deng, Y. B. Dong, B. B. Feng, Q. D. Gao, X. Y.more » Han, W. Y. Hong, M. Huang, X. Q. Ji, Z. H. Kang, D. F. Kong, T. Lan, G. S. Li, H. J. Li, Qing Li, W. Li, Y. G. Li, A. D. Liu, Z. T. Liu, C. W. Luo, X. H. Mao, Y. D. Pan, J. F. Peng, Z. B. Shi, S. D. Song, X. Y. Song, H. J. Sun, A. K. Wang, M. X. Wang, Y. Q. Wang, W. W. Xiao, Y. F. Xie, L. H. Yao, D. L. Yu, B. S. Yuan, K. J. Zhao, G. W. Zhong, J. Zhou, J. C. Yan, C. X. Yu, C. H. Pan, Y. Liu, and the HL-2A Team , Nucl. Fusion 51, 094016 (2011)] to measure the losses of neutral beam ions. The design of the probe is based on the concept of the α-particle detectors on Tokamak Fusion Test Reactor (TFTR) using scintillator plates. The probe is capable of traveling across an equatorial plane port and sweeping the aperture angle rotationally with respect to the axis of the probe shaft by two step motors, in order to optimize the radial position and the collimator angle. The energy and the pitch angle of the lost fast ions can be simultaneously measured if the two-dimensional image of scintillation light intensity due to the impact of the lost fast ions is detected. Measurements of the fast-ion losses using the probe have been performed during HL-2A neutral beam injection discharges. The clear experimental evidence of enhanced losses of beam ions during disruptions has been obtained by means of the SLIP system. A detailed description of the probe system and the first experimental results are reported.« less

The Chemical Constituents and Pharmacological Actions of Cordyceps sinensis

PubMed Central

Liu, Yi; Wang, Jihui; Wang, Wei; Zhang, Hanyue; Zhang, Xuelan; Han, Chunchao

2015-01-01

Cordyceps sinensis, also called DongChongXiaCao (winter worm, summer grass) in Chinese, is becoming increasingly popular and important in the public and scientific communities. This study summarizes the chemical constituents and their corresponding pharmacological actions of Cordyceps sinensis. Many bioactive components of Cordyceps sinensis have been extracted including nucleoside, polysaccharide, sterol, protein, amino acid, and polypeptide. In addition, these constituents' corresponding pharmacological actions were also shown in the study such as anti-inflammatory, antioxidant, antitumour, antiapoptosis, and immunomodulatory actions. Therefore can use different effects of C. sinensis against different diseases and provide reference for the study of Cordyceps sinensis in the future. PMID:25960753

Improving human health outcomes with a low-cost intervention to reduce exposures from lead acid battery recycling: Dong Mai, Vietnam.

PubMed

Ericson, Bret; Duong, Thi To; Keith, John; Nguyen, Trong Cuu; Havens, Deborah; Daniell, William; Karr, Catherine J; Ngoc Hai, Doan; Van Tung, Lo; Thi Nhi Ha, Tran; Wilson, Brian; Hanrahan, David; Croteau, Gerry; Patrick Taylor, Mark

2018-02-01

This study details the first comprehensive evaluation of the efficacy of a soil lead mitigation project in Dong Mai village, Vietnam. The village's population had been subject to severe lead poisoning for at least a decade as a result of informal Used Lead Acid Battery (ULAB) recycling. Between July 2013 to February 2015, Pure Earth and the Centre for Environment and Community Development (Hanoi, Vietnam) implemented a multi-faceted environmental and human health intervention. The intervention consisted of a series of institutional and low-cost engineering controls including the capping of lead contaminated surface soils, cleaning of home interiors, an education campaign and the construction of a work-clothes changing and bathing facility. The mitigation project resulted in substantial declines in human and environmental lead levels. Remediated home yard and garden areas decreased from an average surface soil concentration of 3940mg/kg to <100mg/kg. One year after the intervention, blood lead levels in children (<6 years old) were reduced by an average of 67%-from a median of 40.4μg/dL to 13.3μg/dL. The Dong Mai project resulted in significantly decreased environmental and biological lead levels demonstrating that low-cost, rapid and well-coordinated interventions could be readily applied elsewhere to significantly reduce preventable human health harm. Copyright © 2017 Elsevier Inc. All rights reserved.

Geomorphological diversity of Dong-Sha Atoll based on spectrum and texture analysis in high resolution remote sensing imagery

NASA Astrophysics Data System (ADS)

Chen, Jianyu; Mao, Zhihua; He, Xianqiang

2009-01-01

Coral reefs are complex marine ecosystems that are constructed and maintained by biological communities that thrive in tropical oceans. The Dong-Sha Atoll is located at the northern continental margin of the South China Sea. It has being abused by destructive activity of human being and natural event during recent decades. Remote sensing offers a powerful tool for studying coral reef geomorphology and is the most cost-effective approach for large-scale reef survey. In this paper, the high-resolution Quickbird2 imageries which covered the full atoll are used to categorize the current distribution of coral reefs geomorphological structure therein with the auxiliary SPOT5 and ASTER imageries. Spectral and texture analysis are used to distinguish the geomorphological diversity during data processing. The Gray Level Co-occurrence Matrices is adopted for texture feature extraction and atoll geomorphology mapping in the high-resolution pan-color image of Quickbird2. Quickbird2 is considered as the most appropriate image source for coral reefs studies. In the Dong-Sha Atoll, various dynamical geomorphologic units are developed according to wave energy zones. There the reef frame types are classified to 3 different types according as its diversity at the image. The radial structure system is the most characteristic and from high resolution imagery we can distinguish the discrepancy between them.

The black flies (Diptera: Simuliidae) from Thua Thien Hue and Lam Dong Provinces, Vietnam.

PubMed

Takaoka, Hiroyuki; Sofian-Azirun, Mohd; Ya'cob, Zubaidah; Chen, Chee Dhang; Lau, Koon Weng; Pham, Xuan Da

2015-05-21

Surveys of pupae and larvae of black flies (Diptera: Simuliidae) were carried out in Thua Thien Hue Province of central Vietnam, and Lam Dong Province of southern Vietnam in 2014. A total of 26 species belonging to the genus Simulium were collected, consisting of eight known species, one newly recorded species, and 17 new species (of which three species of the subgenus Nevermannia were described in 2014). The remaining 14 new species (nine of the subgenus Gomphostilbia and five of the subgenus Simulium) are described here based on females, males, pupae and mature larvae. The total number of species of black flies in Vietnam is now 46. Keys to identify all 26 species recorded from the two provinces of Vietnam are given for females, males, pupae and mature larvae.

Study of parameter of nonlinearity in 2-chloroethanol with 2-dimethylethanolamine/2-diethylethanolamine at different temperatures

NASA Astrophysics Data System (ADS)

Awasthi, Anjali; Awasthi, Aashees

2017-06-01

The acoustic non-linearity parameter (B/A) for binary mixtures of 2-chloroethanol with 2-dimethylethanolamine (2-DMAE) and 2-diethylethanolamine (2-DEAE) are evaluated using Tong Dong, Beyer and Beyer-Tong Dong coefficients at varying concentrations and temperatures ranging from 293.15 to 313.15 K. The nonlinearity parameter is used to calculate various molecular properties such as internal pressure, cohesive energy density, Van der waals' constant, distance of closest approach, diffusion coefficient and rotational correlation time. Additionally, the intermediate quantities like temperature and pressure derivatives of sound velocity and phase shift parameter as a function of temperature are also deduced. The extent of intermolecular interactions, anharmonicity and structural configuration of the binaries under investigation are discussed in terms of excess non-linearity parameter (B/A)E.

Transferências orbitais dentro do modelo quase bicircular no sistema Terra-Lua-Sol

NASA Astrophysics Data System (ADS)

Corrêa, A. A.; de Almeida Prado, A. F. B.; Stuchi, T. J.; Beaugé, C.

2003-08-01

O problema quase bicircular planar consiste na determinação da dinâmica de um corpo, de massa desprezível, que se move sob o campo gravitacional de três corpos. No caso estudado, o quarto corpo é considerado um satélite artificial e os demais são a Terra, a Lua e o Sol, formando o sistema de três corpos. Este modelo permite a construção de uma hamiltoniana similar a do problema restrito de três corpos (PRTC), incluindo termos periódicos não autônomos, provenientes da presença do Sol, portanto pode ser entendido como uma perturbação ao PRTC. Suas órbitas periódicas aparecem isoladas e possuem o mesmo período solar. Os pontos de equilíbrio colineares possuem o mesmo caráter de estabilidade linear dos pontos lagrangianos (L1, L2 e L3) do PRTC, sendo do tipo sela x centro x centro, instável na direção x e estáveis nas direções y e z. As órbitas periódicas tridimensionais ao redor do ponto colinear é chamada órbita Halo. O objetivo deste trabalho é determinar órbitas de transferências de baixo consumo de combustível para um veículo espacial partindo de uma órbita de estacionamento ao redor da Terra em direção a uma órbita Halo do ponto L1, situado entre a Terra e Lua. Esta tarefa pode ser transformada no problema de valor de contorno: dado um ponto inicial na órbita de estacionamento, um ponto final na órbita halo e o tempo de transferência, deseja-se obter a trajetória que liga estes dois pontos e que minimize os incrementos de velocidade do veículo. Essas transferências são realizadas baseadas no método de Lambert e analisadas de modo a fornecer um baixo consumo. Posteriormente, é feita uma comparação de consumo com as órbitas de transferência equivalentes do PRTC.

Corrosion and hydrogen pick-up behaviors of cladding and structural components in BWR high burnup 9x9 lead use assemblies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miyashita, Toshiyasu; Nakae, Nobuo; Ogata, Keizo

The high burnup BWR 9x9 lead use fuel assemblies, which have been designed for maximum assembly burnup of 55 GWd/t in Japan, have been examined after irradiations to confirm the reliability of the current safety evaluation methodology, and to accumulate data to judge the adequacy to apply it to the future higher burnup fuel. After 3 and 5 cycle irradiations, post irradiation examinations were performed for both 9x9 Type-A and Type-B fuel assemblies. Both Type LUAs utilize Zry-2 claddings, while there are deviation in the contents of impurity and alloying elements between Type-A and Type-B, especially in Fe and Simore » concentration. Measured oxide thicknesses of fuel rods showed no significant difference between after 3 and 5 cycle irradiation except for some rods at corner position in Type B LUA. The axial profile of hydrogen concentration and oxide thickness for the corner rods in Type B LUA after 5 cycle irradiation had peaks at the second lowest span from the bottom. The maximum oxide thickness is about 50 {mu}m on the surface facing the bundle outside at the second lowest span and dense hydrides layer (Hydride rim) is observed in peripheral region of cladding showing unexpected high hydrogen concentration. The results of calculated thermal-hydraulic conditions show that the thermal neutron flux at the corner position was higher than the other position. On the other hand, the void fraction and the mass flux were relatively lower at the corner position. The oxide thickness on spacer band and spacer cell of Zry-2 increases from 3 to 5 cycle irradiations. Spacer band of Zry-4 showed significantly thick oxide after 5 cycle irradiations but Hydrogen concentration was relatively small in contrast its obviously thick oxide in comparison with Zry-2 spacer bands. The large increase in hydrogen concentration was measured in Zry-2 spacers after 5 cycle irradiations and the evaluated hydrogen pick-up rate also increased remarkably. (authors)« less

Correlation of Shear Strength Between Longitudial and Transverse Specimens

DTIC Science & Technology

2012-05-17

Transverse Specimens" (2012). University of New Orleans Theses and Dissertations. Paper 1435. http://scholarworks.uno.edu/ td /1435 Report Documentation... ATI ) and they were  the sole providers of this metal during this project.  ATI  called the alloy:  ATI  425; it is very similar  in its properties to...Nie, C.; Dong, P.. Traction Stress Based Shear Strength Definition of Fillet Welds. Pending  Publishing.  [5] ATI  38‐644 Beta Titanium Alloy. Technical

Quality Evaluation of Coatings by Automatic Scratch Testing

DTIC Science & Technology

1989-11-01

MTL TR 89-98 IADII QUALITY EVALUATION OF COATINGS BY AUTOMATIC SCRATCH TESTING KIRIT J. BHANSALI LBRTR A1 U.S. ARMY MATERIALS TECHNOLOGY LABORATORY...distribution unlimited. LABORATORY COMMANO U.S. ARMY MATERIALS TECHNOLOGY LABORATORY PMUNKS wcamaauv LUaAMUv Watertown, Massachusetts 02172-0001 .o...Theo 7- Kattamis* 9 PEWNWING ORGANIZATION NAME AMO ADDRESS 1.PORUEEET RJC.TS AREA & WORK UNIT NUMSS U.S. Army Materials Technology Laboratory Watertown

[Professor DONG Gui-rong's experience for the treatment of peripheral facial paralysis].

PubMed

Cao, Lian-Ying; Shen, Te-Li; Zhang, Wei; Chen, Si-Hui

2012-05-01

Professor DONG Gui-rong's theoretical principle and manipulation points for peripheral facial paralysis were introduced in details from the angels of syndrome differentiation, timing, acupoint prescription and needling methods. For the syndrome differentiation and timing, the professor emphasized to check the treatment timing and follow the symptoms, which should be treated by stages, besides, it was necessary to find and distinguish the reason and nature of diseases to have a combined treatment of tendons and muscles. For the acupoint prescription and needling methods, he has proposed that the acupoints selection should be compatible of distal and lacal, and made a best of Baihui (GV 20) to regulate the whole yang qi, also he has paid much attention to the needling methods and staging treatment. Under the consideration of late stage of peripheral facial paralysis, based on syndrome differentiation Back-shu points have been selected to regulate zang-fu function, should achieve much better therapeutic effect.

Palaeoflood records of the last three centuries from the Pyeongchang and Dong rivers, South Korea

NASA Astrophysics Data System (ADS)

Kim, Song-Hyun; Tanaka, Yukiya

2017-08-01

Slackwater deposits are paleostage indicators in paleoflood hydrology that have commonly been used in numerous studies to estimate the magnitude and frequency of flood events and to reconstruct paleoenvironments and paleohydrology. In this study, individual flood events along the Pyeongchang (PC) and Dong (D) rivers of South Korea were differentiated on the basis of changes in deposit color, grain size, organic content, and the existence of laminations. Based on 137Cs and 14C chronological data, 19 flood events have occurred at site PC since 1720, while 17 flood events have occurred at site D since 1815. At the PC study site, the average time interval between the flood events is 15.4 years and the average sediment accumulation rate is 9.7 mm/y. At study site D, the average time interval is 15 years and the average sediment accumulation rate is 11.6 mm/y. These high sediment accumulation rates are consistent with those in humid areas (e.g., Japan) and explain how slackwater deposits can be preserved despite erosion, bioturbation, and pedogenic processes. Based on the results, the study area was divided into three periods: (1) a relatively wet period (1720-1810 CE), (2) a dry period (1810-1960 CE), and (3) a wet period (1960 CE-present). The flood time intervals and average sediment accumulation rates of the eighteenth century were shorter and higher than those of the nineteenth century. This suggests that on the Korean Peninsula, the paleoclimate of the Little Ice Age (LIA) was wetter than that from the nineteenth century to the early twentieth century.

Bacterially produced human B7-1 protein encompassing its complete extracellular domain maintains its costimulatory activity in vitro.

PubMed

Shen, W; Wang, Y; Geng, Y; Si, L

2000-08-01

To investigate which of the two immunoglobulin (Ig)-like domains, immunoglobulin variable region homologous domain IgV (hB7-1 IgV), or immunoglobulin constant region homologous domain IgC (hB7-1 IgC) on human B7-1 molecule contain the receptor binding sites, and to evaluate if the B7-1 molecule expressed in bacteria has biological activity. PCR was used to amplify three fragments of hB7-1 IgV, hB7-1 IgC and complete extracellular region of human B7-1 containing both the IgV and IgC domains (hB7-1 IgV + IgC). Three recombinants, pQE9-hB7-1 IgV, pQE9-hB7-1 IgC and pQE9-Hb7-1 (IgV + IgC) were generated by cloning the PCR products into a prokaryote expression plasmid (pQE-9) and were introduced into the host stain M15. The relevant target hexahistidine-tagged proteins were identified by SDS-PAGE and Western blotting. With the presence of the first signal imitated by anti-CD3 antibody, T cell activation was observed by exposing purified T lymphocytes to each soluble form of the three bacterially-produced human B7-1 proteins and [3H]-TdR incorporation. Three recombinant proteins of human B7-1, hB7-1 IgV, hB7-1 IgC and hB7-1 (IgV + IgC) were produced and detected in both soluble and inclusive body forms from engineered bacterial cells. With the presence of anti-CD3 antibody, T lymphocytes proliferated when co-stimulated by bacterially produced hB7-1 (IgV + IgC), but not by either hB7-1 IgV or hB7-1 IgC. Functional glycoprotein human B7-1 could be produced in bacterial cells. Both extracellular immunoglobulin-like domains are necessary for B7-1 to react with its counter receptors.

A Virtual Reality Exposure Therapy Application for Iraq War Post Traumatic Stress Disorder

DTIC Science & Technology

2006-01-01

denial of social problems. Prior to the availability of VR therapy applications, the existing standard of care for PTSD was imaginal exposure...The application is built on ICT’s FlatWorld Simulation Control Architecture (FSCA) [13]. The FSCA enables a network -centric system of client displays...client-side interaction despite potential network delays. FCSA scripting is based on the Lua programming language [14] and provides facilities for real

Androgen Receptor Splice Variants and Resistance to Taxane Chemotherapy

DTIC Science & Technology

2017-10-01

presence or absence of androgen. Citations (published journal articles): Xichun Liu, Elisa Ledet, Dongying Li, Ary Dotiwala, Allie Steinberger...paper was published: Xichun Liu, Elisa Ledet, Dongying Li, Ary Dotiwala, Allie Steinberger, Jianzhuo Li, Yanfeng Qi, Yan Dong, Jonathan Silberstein

Kansas Working Papers in Linguistics, Volume 20.

ERIC Educational Resources Information Center

Goodell, Melissa, Ed.; Choi, Dong-Ik, Ed.

1995-01-01

Papers in this volume include the following: "Evidence for Foot Structure in Hausa" (Ousseina Alidou); "Korean 'Tense' Consonants as Geminates" (Dong-Ik Choi); "Gemination Processes: Motivation, Form, and Constraints" (Mamadou Niang); "Syllable 'Sonority' Hierarchy and Pulaar Stress: A Metrical Approach"…

14 CFR 71.51 - Class C airspace.

Code of Federal Regulations, 2013 CFR

2013-01-01

... DESIGNATION OF CLASS A, B, C, D, AND E AIRSPACE AREAS; AIR TRAFFIC SERVICE ROUTES; AND REPORTING POINTS Class C Airspace § 71.51 Class C airspace. The Class C airspace areas listed in subpart C of FAA Order... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Class C airspace. 71.51 Section 71.51...

14 CFR 71.51 - Class C airspace.

Code of Federal Regulations, 2010 CFR

2010-01-01

... DESIGNATION OF CLASS A, B, C, D, AND E AIRSPACE AREAS; AIR TRAFFIC SERVICE ROUTES; AND REPORTING POINTS Class C Airspace § 71.51 Class C airspace. The Class C airspace areas listed in subpart C of FAA Order... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Class C airspace. 71.51 Section 71.51...

Expression of fusion IL2-B7.1(IgV+C) and effects on T lymphocytes.

PubMed

Kong, Linghong; Li, Yaochen; Yang, Ye; Li, Kangsheng

2007-12-01

The search for an effective immunotherapeutic treatment for tumors is an important area of cancer research. To prepare a more effective form of the bifunctional fusion protein IL2-B7.1(IgV+C) and analyze its effect on the stimulation of T lymphocyte proliferation, we used DNAStar 5.03 software to predict the structural diversity and biochemical character of IL2-B7.1(IgV+C). We then prepared fusion protein IL2-B7.1(IgV+C) by establishing its prokaryotic expression system, and tested its effect on the stimulation of T lymphocytes in vitro. The results indicated that IL2-B7.1(IgV+C) correctly formed a secondary structure in which both IL2 and B7.1(IgV+C) maintained their original hydrophilicity and epitopes. Western blot analysis revealed that IL2-B7.1(IgV+C) was efficiently expressed. Our analysis of CTLL-2 and T-cell proliferation showed that recombinant human (rh) IL2-B7.1(IgV+C) exerted the combined stimulating effects of both rhIL2 and rh B7.1(IgV+C) on cell proliferation, and that these effects could be blocked by adding either anti-IL2 or anti-B7.1 monoclonal antibodies. A >2-fold increase in [3H]TdR incorporation compared with that of cells treated with recombinant protein IL2, or B7.1(IgV+C) alone, revealed that rhIL2-B7.1(IgV+C) had dose-dependent synergetic effects on T-cell activation in the presence of anti-CD3 monoclonal antibody. We concluded that the augmented potency of rhIL2-B7.1(IgV+C) resulted in a stronger stimulation of T-cell proliferation than either rhB7.1(IgV+C) or rhIL2 alone.

Development of High Cordycepin-Producing Cordyceps militaris Strains.

PubMed

Kang, Naru; Lee, Hyun-Hee; Park, Inmyoung; Seo, Young-Su

2017-03-01

Cordyceps militaris , known as Dong-Chong-Xia-Cao, produces the most cordycepin among Cordyceps species and can be cultured artificially. For these reasons, C. militaris is widely used as herb or functional food in the East Asia. In this study, we developed a new strain of C. militaris that produces higher cordycepin content than parent strains through mating-based sexual reproduction. Twenty parent strains were collected and identified as C. militaris based on internal trasncrived spacer and rDNA sequences. Seven single spores of MAT 1-1 idiomorph and five single spores of MAT 1-2 idiomorph were isolated from 12 parent strains. When 35 combinations were mated on the brown rice medium with the isolated single spores, eight combinations formed a stroma with a normal perithecia and confirmed mated strains. High pressure liquid chromatography analysis showed that mated strain KSP8 produced the most cordycepin in all the media among all the tested strains. This result showed due to genetic recombination occurring during the sexual reproduction of C. militaris . The development of C. militaris strain with increased cordycepin content by this approach can help not only to generate new C. militaris strains, but also to contribute to the health food or medicine industry.

Development of High Cordycepin-Producing Cordyceps militaris Strains

PubMed Central

Kang, Naru; Lee, Hyun-Hee; Park, Inmyoung

2017-01-01

Cordyceps militaris, known as Dong-Chong-Xia-Cao, produces the most cordycepin among Cordyceps species and can be cultured artificially. For these reasons, C. militaris is widely used as herb or functional food in the East Asia. In this study, we developed a new strain of C. militaris that produces higher cordycepin content than parent strains through mating-based sexual reproduction. Twenty parent strains were collected and identified as C. militaris based on internal trasncrived spacer and rDNA sequences. Seven single spores of MAT 1-1 idiomorph and five single spores of MAT 1-2 idiomorph were isolated from 12 parent strains. When 35 combinations were mated on the brown rice medium with the isolated single spores, eight combinations formed a stroma with a normal perithecia and confirmed mated strains. High pressure liquid chromatography analysis showed that mated strain KSP8 produced the most cordycepin in all the media among all the tested strains. This result showed due to genetic recombination occurring during the sexual reproduction of C. militaris. The development of C. militaris strain with increased cordycepin content by this approach can help not only to generate new C. militaris strains, but also to contribute to the health food or medicine industry. PMID:28435352

JPRS Report East Asia Southeast Asia

DTIC Science & Technology

1993-03-25

particularly in the processing industry. Land and climate conditions are suitable for short and long term industries such as rubber, coffee, cashew nuts ...investment of existing companies by developing and processing rubber, paper, coffee and cashew nuts . Dong Nai has also begun exploiting marble and

Taking a Cultural-Response Approach to Teaching Multicultural Literature

ERIC Educational Resources Information Center

Yu Ren Dong

2005-01-01

Yu Ren Dong presents several activities that can give teachers confidence to explore the cultural differences in diverse texts and provide ways to help their students discuss these differences and enhance cross-cultural understanding. The use of cultural-response approach is highlighted.

JPRS Report, China

DTIC Science & Technology

1989-10-25

soy sauce, vinegar , and sauces), edible oil products (salad oil, margarine, and cheese oil), vegetable protein products and various kinds of bean... fermenting this "bitter wine" of calamity. Dong Yulin [5516 3768 7792], a cadre at Beijing Yanhua Petroleum Corporation who is also eastern division vice

Assessment of polycyclic aromatic hydrocarbons (PAHs) pollution in soil of suburban areas in Tianjin, China.

PubMed

Lv, Jungang; Shi, Rongguang; Cai, Yanming; Liu, Yong

2010-07-01

Soil contamination with polycyclic aromatic hydrocarbons is an increasing problem and has aroused more and more concern in many countries, including China. In this study, representative soil samples (n = 87) of suburban areas in Tianjin (Xiqing, Dongli, Jinnan, Beichen) were evaluated for 16 polycyclic aromatic hydrocarbons. Surface soil samples were air-dried and sieved. Microwave assisted extraction was used for polycyclic aromatic hydrocarbons preparation prior to analysis with gas chromatography-mass spectrometry. The total concentrations of tested polycyclic aromatic hydrocarbons in Xiqing, Dongli, Jinnan, Beichen ranged in 58.5-2,748.3, 36.1-6,734.7, 58.5-4,502.5, 29.7-852.5 ng/g and the averages of total concentration of polycyclic aromatic hydrocarbons were 600.5, 933.6, 640.8, 257.3 ng/g, respectively. Spatial variation of polycyclic aromatic hydrocarbons in soil was illustrated; Pollution status and comparison to other cities were also investigated. Serious polycyclic aromatic hydrocarbons soil pollution was found in Dongli district, on the contrary, Bap in most sites in Beichen did not exceed relative standards and most sites in Beichen should be classified as non-contaminated soil. Principal component analysis was used to identify the possible sources of different districts. It turned out that coal combustion still was the most important sources in three districts except Beichen. Coking, traffic, cooking, biomass combustion also accounted for polycyclic aromatic hydrocarbons pollution to certain extent in different districts. These data can be further used to assess the health risk associated with soils polluted with polycyclic aromatic hydrocarbons and help local government find proper way to reduce polycyclic aromatic hydrocarbons pollution in soils.

High-Pressure Granulite Facies Overprinting During the Exhumation of Eclogites in the Bangong-Nujiang Suture Zone, Central Tibet: Link to Flat-Slab Subduction

NASA Astrophysics Data System (ADS)

Zhang, Xiu-Zheng; Wang, Qiang; Dong, Yong-Sheng; Zhang, Chunfu; Li, Qing-Yun; Xia, Xiao-Ping; Xu, Wang

2017-12-01

The geometric transformation of a descending plate, such as from steep to flat subduction in response to a change from normal to overthickened oceanic crust during subduction, is a common and important geological process at modern or fossil convergent margins. However, the links between this process and the metamorphic evolution of the exhumation of oceanic (ultra)high-pressure eclogites are poorly understood. Here we report detailed petrological, mineralogical, phase equilibria, and secondary ion mass spectrometry zircon and rutile U-Pb age data for the Dong Co eclogites at the western segment of the Bangong-Nujiang suture zone, central Tibet. Our data reveal that the Dong Co eclogites experienced peak eclogite-facies metamorphism (T = 610-630°C, P = 2.4-2.6 GPa) and underwent multiple stages of retrograde metamorphism. P-T pseudosections and compositional isopleths of garnet define a complex clockwise P-T-t path (including two stages of decompression-dominated P-T path and one of isobaric heating), suggesting varying exhumation velocities. Combining previous studies with our new results, we suggest that the transformation from rapid to slow exhumation is dominated by the transition from steep to flat subduction. The flat-slab segment, caused by subduction of buoyant oceanic plateau, led to an extremely slow exhumation and a strong overprinting of HP granulite facies at a depth of 50 km at 177 Ma. The slab rollback that followed in response to a substantial density increase of the eclogitized oceanic plateau resulted in another rapid exhumation process at 168 Ma and triggered the formation of abundant near-simultaneous or later magmatic rocks.

Study on Inhibitory Effect of MaiMenDong Decoction and WeiJing Decoction Combination with Cisplatin on NCI-A549 Xenograft in Nude Mice and Its Mechanism.

PubMed

Xiong, Fei; Jiang, Miao; Chen, Meijuan; Wang, Xiaoxia; Zhang, Shiping; Zhou, Jing; Li, Ke; Sheng, Yan; Yin, Lian; Tang, Yuping; Ye, Lihong; Wu, Mianhua; Fu, Haian; Zhang, Xu

2017-01-01

MaiMenDong Decoction and WeiJing Decoction (Jin formula) is a traditional Chinese medication that consists of 8 medicinal plants, which recorded in the classical TCM literature Jin Kui Yao Lue and has been utilized in the treatment of lung diseases for hundreds of years in China. The present study aimed to determine the anti-tumor activity and the underlying mechanisms of Jin formula combined with cisplatin in the treatment of non-small cell lung cancer (NSCLC). Xenograft model of NCI-A549 was established in Balb/c nude mice. Five groups, including normal, MOCK, Jin, cisplatin (DDP), and Jin+DDP were included in the study. We found that Jin formula ameliorated the body weight loss caused by DDP 15 days after drug administration. Moreover, the combination of Jin with DDP enhanced the anti-tumor function of DDP. Microarray analysis showed that Jin suppressed gene expression of certain pathways which regulating cell cycle and apoptosis. Furthermore, DDP mainly decreased the gene expression level of angiogenesis associated factors, such as VEGFA, TGF-β and MMP-1. Moreover, co-treatment with Jin and DDP not only down-regulated Bcl-2 and E2F1, but also decreased the expression of MYC, MET, and MCAM. In addition, co-formula decreased the levels of p-AKT (thr308) and p-PTEN, increased Bax/Bcl-2 value, and resulted in apoptosis of tumor cells. Taken together , Jin+DDP significantly inhibited the growth of A549 cell transplanted solid tumor with slight side effect compared to the treatment by DDP only, and had a better effect than the Jin group. The mechanisms may be mainly associated with inactivation of PI3K/AKT pathway and apoptosis induction.

CrossTalk: The Journal of Defense Software Engineering. Volume 20, Number 10, October 2007

DTIC Science & Technology

2007-10-01

ch ...Re vie w Va lua tio n Ar ch ite cti ng De ve lop m en t 1 Ar ch ite cti ng 2 Ex plo ra tio n Op er at ion 1 De ve lop m en t 2 Ar ch ite cti ng 3...engineering systems from MIT. MIT 77 Massachusetts AVE NE20-388 Cambridge, MA 02139 Phone: (617) 324-0473 Fax: (617) 258- 7845 E-mail:

CUHK Papers in Linguistics, Number 3.

ERIC Educational Resources Information Center

Yip, Virginia, Ed.

1991-01-01

Papers in this volume include the following: "Constraints on Dative Acquisition by Chinese ESL Learners" (Hua Dong Fan); "The Learnability of Locality Conditions on Quantification" (Thomas Lee); "Do Learning Environments Make a Difference? A Study on the Acquisition of the English Interrogatives by Three Types of Cantonese…

76 FR 39897 - In the Matter of Certain Liquid Crystal Display Devices and Products Containing the Same; Notice...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-07

...-10, Seocho 2-dong, Seocho-gu, Seoul, Korea 137- 857. (b) The respondents are the following entities...: AU Optronics Corp., No. 1 Li-Hsin Road 2, Hsinchu Science Park, Hsinchu 30078, Taiwan. AU Optronics...

The Lao Dong Party of North Vietnam

DTIC Science & Technology

1960-06-17

and consol- idation of the cooperative movement and with the achievement of the task of reforming democracy in the mountain regions. .. . During the...that, on the overall picture of the enlarged cooperative movement , we must continue to improve the degree of enlightenment about socialism of the

Charaterizing the O+ ion plume from Hybrid simulations: comparison to MAVEN observations

NASA Astrophysics Data System (ADS)

Modolo, R.; Leblanc, F.; Chaufray, J. Y.; Leclercq, L.; Esteban-Hernandez, R.; Curry, S.; Dong, Y.; Brain, D. A.; Bowers, C.; Luhmann, J. G.; McFadden, J. P.; Halekas, J. S.; Espley, J. R.; Connerney, J. E. P.; Jakosky, B. M.

2015-12-01

MAVEN observations show a substantial plume-like distribution of escaping ions from the Martian atmosphere. It represents an important ion escape channel with large fluxes (Brain et al, 2015; Dong et al, 2015, Curry et al, 2015). Such structure is organized by the solar wind convection electric field and it is located in the MSE northward hemisphere. Global hybrid models (eg Modolo et al, 2005, 2012; Kallio et al, 2006; Brecht et al, 2006) reproduce nicely this plume. To further characterize this population, hybrid simulations have been performed with upstream solar wind conditions observed by MAVEN. Simulation results along the spacecraft track present signatures of high energetic O+ ions similar to MAVEN measurements. Comparison of simulated 3D distribution functions of this population are compared to STATIC and SWIA observations. Moreover a comparison of hybrid results with statistical ion fluxes maps derived from MAVEN (Dong et al, 2015; Brain et al, 2015) have been conducted and a reasonable agreement is found .

Clinically isolated enterovirus A71 subgenogroup C4 strain with lethal pathogenicity in 14-day-old mice and the application as an EV-A71 mouse infection model.

PubMed

Xu, Yi; Ma, Shuzhi; Zhu, Limeng; Huang, Zhiqiu; Chen, Liyun; Xu, Yuhua; Yin, Haibin; Peng, Tao; Wang, Yi

2017-01-01

The Enterovirus A71 (EV-A71) subgenogroup C4 is prevalent in China. EV-A71 causes hand, foot and mouth disease (HFMD) in children and may lead to severe neurological diseases. The development of antiviral and protective vaccines against EV-A71 is significantly hindered by the lack of suitable animal models to recapitulate human neurological symptoms. In this study, GZ-CII, a highly virulent EV-A71 subgenogroup C4 strain, was isolated from hospitalized children with HFMD. Intraperitoneal infections of GZ-CII resulted in progressive neurological disease in mice as old as 14 days. Administration of an inactivated EV-A71 vaccine or an anti-EV-A71 immune serum protected the mice against the GZ-CII infection. This demonstrated that a mouse model with EV-A71 GZ-CII could be used to evaluate potential vaccine candidates and therapeutics for subgenogroup C4. Comparing the genome sequence of GZ-CII with that of the avirulent EV-A71 subgenogroup C4 strain revealed unique mutations in GZ-CII. When mutation VP2-K149I was introduced into the nonpathogenic EV-A71 subgenogroup C4 strain, the variant similar to GZ-CII significantly increased viral replication and virulence in mice. These results indicated that the VP2-K149I mutation played an important role in enhancing the virulence of the EV-A71 subgenogroup C4 strain in mice, and that mice infected with the GZ-CII strain are a promising model for evaluating vaccines and therapeutics against the EV-A71 subgenogroup C4. Copyright © 2016 Elsevier B.V. All rights reserved.

Genomic region operation kit for flexible processing of deep sequencing data.

PubMed

Ovaska, Kristian; Lyly, Lauri; Sahu, Biswajyoti; Jänne, Olli A; Hautaniemi, Sampsa

2013-01-01

Computational analysis of data produced in deep sequencing (DS) experiments is challenging due to large data volumes and requirements for flexible analysis approaches. Here, we present a mathematical formalism based on set algebra for frequently performed operations in DS data analysis to facilitate translation of biomedical research questions to language amenable for computational analysis. With the help of this formalism, we implemented the Genomic Region Operation Kit (GROK), which supports various DS-related operations such as preprocessing, filtering, file conversion, and sample comparison. GROK provides high-level interfaces for R, Python, Lua, and command line, as well as an extension C++ API. It supports major genomic file formats and allows storing custom genomic regions in efficient data structures such as red-black trees and SQL databases. To demonstrate the utility of GROK, we have characterized the roles of two major transcription factors (TFs) in prostate cancer using data from 10 DS experiments. GROK is freely available with a user guide from >http://csbi.ltdk.helsinki.fi/grok/.

Providing Meaningful Learning for Students of the Sixth Grade of Middle School: a Study on the Moon Phases. (Breton Title: Propiciando Aprendizagem Significativa Para Alunos do Sexto Ano do Ensino Fundamental: um Estudo sobre as Fases da Lua.) Propiciando el Aprendizaje Significativo Para Alumnos del Sexto Nivel de la Educación General Básica: un Estudio sobre Las Fases de la Luna

NASA Astrophysics Data System (ADS)

Darroz, Luiz Marcelo; Samudio Pérez, Carlos Ariel; da Rosa, Cleci Werner; Heineck, Renato

2012-07-01

We relate in this article a didactic experience studying the moon phases with a group of middle school students of a private school of the municipality of Passo Fundo, RS. Based on David Ausubel's Meaningful Learning Theory, we have sought to develop a proposal following a didactic model which simulates the phases of the Moon, as based on the previous conceptions of the students. The signs of learning were evidenced by means of memory registries of the activity. From the obtained results we believe that the proposal achieved its goals, since the students were able to identify, differentiate and transfer the phenomenon of the moon phases to new contexts. Thus, it is concluded that a methodology focused on a meaningful content for the students is fundamental to the construction and genuine grasping of what is being learned. Neste artigo, relata-se uma experiência didática de estudo das fases da Lua com uma turma do 6° ano do Ensino Fundamental, de uma escola privada do município de Passo Fundo, RS. Tendo como fundamentação teórica a Teoria da Aprendizagem Significativa de David Ausubel, buscou-se desenvolver a proposta a partir de um modelo didático que simula as fases da Lua e com base nas concepções prévias dos estudantes. Os indícios da aprendizagem foram constatados através de registros de memórias da atividade. Pelos resultados apresentados, acredita-se que a proposta alcançou seus objetivos, uma vez que os estudantes conseguiram identificar, diferenciar e transferir o fenômeno das fases da Lua para novos contextos. Assim, conclui-se que uma metodologia com enfoque em um conteúdo significativo ao estudante é fundamental para a construção e compreensão genuína do que está sendo aprendido. En este artículo se relata una experiencia didáctica de estudio de las fases de la Luna con una clase de 6º año de la educación general básica de una escuela privada del municipio de Passo Fundo, RS. Teniendo como fundamentación teórica la Teoría del Aprendizaje Significativo de David Ausubel, se buscó desenvolver la propuesta a partir de un modelo didáctico que simula las Fases de la Luna, usando como base las concepciones previas de los estudiantes. Los indicios del aprendizaje fueron verificados a través de registros de memorias de la actividad. Por los resultados obtenidos creemos que la propuesta alcanzó sus objetivos, una vez que los estudiantes consiguieron identificar y transferir el fenómeno de las fases de la Luna para nuevos contextos. Así, se concluye que una metodología con enfoque en un contenido significativo para el estudiante es fundamental para la construcción y comprensión genuina de lo que está siendo aprendido.

Estimating Energy Efficiency of Connected and Autonomous Vehicles in a Mixed Fleet

DOT National Transportation Integrated Search

2018-05-01

Jing Dong (orcid.org/0000-0002-7304-8430); Liang Hu (orcid.org/0000-0001-6351-8542); Chaoru Lu (orcid.org/0000-0001-8418-7658); Connected and autonomous vehicle (CAV) technologies are likely to be gradually implemented over time and in a traffic envi...

Unacceptably High Mortality Related to Measles Epidemics in Niger, Nigeria, and Chad

PubMed Central

Grais, R. F; Dubray, C; Gerstl, S; Guthmann, J. P; Djibo, A; Nargaye, K. D; Coker, J; Alberti, K. P; Cochet, A; Ihekweazu, C; Nathan, N; Payne, L; Porten, K; Sauvageot, D; Schimmer, B; Fermon, F; Burny, M. E; Hersh, B. S; Guerin, P. J

2007-01-01

Background Despite the comprehensive World Health Organization (WHO)/United Nations Children's Fund (UNICEF) measles mortality–reduction strategy and the Measles Initiative, a partnership of international organizations supporting measles mortality reduction in Africa, certain high-burden countries continue to face recurrent epidemics. To our knowledge, few recent studies have documented measles mortality in sub-Saharan Africa. The objective of our study was to investigate measles mortality in three recent epidemics in Niamey (Niger), N'Djamena (Chad), and Adamawa State (Nigeria). Methods and Findings We conducted three exhaustive household retrospective mortality surveys in one neighbourhood of each of the three affected areas: Boukoki, Niamey, Niger (April 2004, n = 26,795); Moursal, N'Djamena, Chad (June 2005, n = 21,812); and Dong District, Adamawa State, Nigeria (April 2005, n = 16,249), where n is the total surveyed population in each of the respective areas. Study populations included all persons resident for at least 2 wk prior to the study, a duration encompassing the measles incubation period. Heads of households provided information on measles cases, clinical outcomes up to 30 d after rash onset, and health-seeking behaviour during the epidemic. Measles cases and deaths were ascertained using standard WHO surveillance-case definitions. Our main outcome measures were measles attack rates (ARs) and case fatality ratios (CFRs) by age group, and descriptions of measles complications and health-seeking behaviour. Measles ARs were the highest in children under 5 y old (under 5 y): 17.1% in Boukoki, 17.2% in Moursal, and 24.3% in Dong District. CFRs in under 5-y-olds were 4.6%, 4.0%, and 10.8% in Boukoki, Moursal, and Dong District, respectively. In all sites, more than half of measles cases in children aged under 5 y experienced acute respiratory infection and/or diarrhoea in the 30 d following rash onset. Of measles cases, it was reported that 85.7% (979/1,142) of patients visited a health-care facility within 30 d after rash onset in Boukoki, 73.5% (519/706) in Moursal, and 52.8% (603/1,142) in Dong District. Conclusions Children in these countries still face unacceptably high mortality from a completely preventable disease. While the successes of measles mortality–reduction strategies and progress observed in measles control in other countries of the region are laudable and evident, they should not overshadow the need for intensive efforts in countries that have just begun implementation of the WHO/UNICEF comprehensive strategy. PMID:17199407

26 CFR 1.501(c)(7)-1 - Social clubs.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 26 Internal Revenue 7 2011-04-01 2009-04-01 true Social clubs. 1.501(c)(7)-1 Section 1.501(c)(7)-1...) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(c)(7)-1 Social clubs. (a) The exemption provided by... general, this exemption extends to social and recreation clubs which are supported solely by membership...

26 CFR 1.501(c)(7)-1 - Social clubs.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 26 Internal Revenue 7 2014-04-01 2013-04-01 true Social clubs. 1.501(c)(7)-1 Section 1.501(c)(7)-1...) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(c)(7)-1 Social clubs. (a) The exemption provided by... general, this exemption extends to social and recreation clubs which are supported solely by membership...

26 CFR 1.501(c)(7)-1 - Social clubs.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Social clubs. 1.501(c)(7)-1 Section 1.501(c)(7)-1...) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(c)(7)-1 Social clubs. (a) The exemption provided by... general, this exemption extends to social and recreation clubs which are supported solely by membership...

New Introductions of Enterovirus 71 Subgenogroup C4 Strains, France, 2012

PubMed Central

Henquell, Cécile; Mirand, Audrey; Coste-Burel, Marianne; Marque-Juillet, Stéphanie; Desbois, Delphine; Lagathu, Gisèle; Bornebusch, Laure; Bailly, Jean-Luc; Lina, Bruno

2014-01-01

In France during 2012, human enterovirus 71 (EV-A71) subgenogroup C4 strains were detected in 4 children hospitalized for neonatal fever or meningitis. Phylogenetic analysis showed novel and independent EV-A71 introductions, presumably from China, and suggested circulation of C4 strains throughout France. This observation emphasizes the need for monitoring EV-A71 infections in Europe. PMID:25061698

New Horizons in Education, 2003.

ERIC Educational Resources Information Center

Ho, Kwok Keung, Ed.

2003-01-01

This journal, written in English and Chinese, includes the following papers: "Values for Creativity: A Study among Undergraduates in Hong Kong and Guangzhou" (Xia Dong Yue and Kok Leung); "The Present Situation of Family Education at the Turn of the Century: An Investigation in Three South-Eastern Provinces in China" (Feng…

Using Nanotechnology to Detect Nerve Agents

DTIC Science & Technology

2011-01-01

56 | Air & Space Power Journal Air Force Institute of Technology Using Nanotechnology to Detect Nerve Agents Lt Col Mark N. Goltz , PhD, USAF...Retired Dr. Dong Shik Kim Maj LeeAnn Racz, PhD, USAF* *Lieutenant Colonel Goltz and Major Racz are faculty members in the Department of Systems and

Evolution and phylogeographic dissemination of endemic porcine picornaviruses in Vietnam

PubMed Central

Lu, Lu; Van Dung, Nguyen; Bryant, Juliet E.; Carrique-Mas, Juan; Van Cuong, Nguyen; Anh, Pham Honh; Rabaa, Maia A.; Baker, Stephen; Simmonds, Peter; Woolhouse, Mark E.

2016-01-01

Members of the Picornaviridae are important and often zoonotic viruses responsible for a variety of human and animal diseases. However, the evolution and spatial dissemination of different picornaviruses circulating in domestic animals are not well studied. We examined the rate of evolution and time of origin of porcine enterovirus G (EV-G) and porcine kobuvirus species C lineages (PKV-C) circulating in pig farms in Vietnam and from other countries. We further explored the spatiotemporal spread of EV-G and PKV-C in Southwest Vietnam using phylogeographic models. Multiple types of EV-G are co-circulating in Vietnam. The two dominant EV-G types among isolates from Vietnam (G1 and G6) showed strong phylogenetic clustering. Three clades of PKV-C (PKV-C1-3) represent more recent introductions into Vietnam; PKV-C2 is closely related to PKV-C from Southwest China, indicating possible cross-border dissemination. In addition, high virus lineage migration rates were estimated within four districts in Dong Thap province in Vietnam for both EV-G types (G1, G6) and all PKV-C (C1-3) clades. We found that Chau Thanh district is a primary source of both EV-G and PKV-C clades, consistent with extensive pig trading in and out of the district. Understanding the evolution and spatial dissemination of endemic picornaviruses in pigs may inform future strategies for the surveillance and control of picornaviruses. PMID:27774295

Generic Space Science Visualization in 2D/3D using SDDAS

NASA Astrophysics Data System (ADS)

Mukherjee, J.; Murphy, Z. B.; Gonzalez, C. A.; Muller, M.; Ybarra, S.

2017-12-01

The Southwest Data Display and Analysis System (SDDAS) is a flexible multi-mission / multi-instrument software system intended to support space physics data analysis, and has been in active development for over 20 years. For the Magnetospheric Multi-Scale (MMS), Juno, Cluster, and Mars Express missions, we have modified these generic tools for visualizing data in two and three dimensions. The SDDAS software is open source and makes use of various other open source packages, including VTK and Qwt. The software offers interactive plotting as well as a Python and Lua module to modify the data before plotting. In theory, by writing a Lua or Python module to read the data, any data could be used. Currently, the software can natively read data in IDFS, CEF, CDF, FITS, SEG-Y, ASCII, and XLS formats. We have integrated the software with other Python packages such as SPICE and SpacePy. Included with the visualization software is a database application and other utilities for managing data that can retrieve data from the Cluster Active Archive and Space Physics Data Facility at Goddard, as well as other local archives. Line plots, spectrograms, geographic, volume plots, strip charts, etc. are just some of the types of plots one can generate with SDDAS. Furthermore, due to the design, output is not limited to strictly visualization as SDDAS can also be used to generate stand-alone IDL or Python visualization code.. Lastly, SDDAS has been successfully used as a backend for several web based analysis systems as well.

Proving the Orbits of the Galilean Moons Through Astrophotography. (Spanish Title: Comprobando Las Órbitas de Las Lunas Galileanas a Través de la Astrofotografía.) Evidenciando as Órbitas das Luas Galileanas Atravéd da Astrofotografia

NASA Astrophysics Data System (ADS)

Iachel, Gustavo

2009-12-01

An activity related to the observation of the Galilean moons and to amateur astrophotography is presented in this work. Through the reading of excerpts of the book Sidereus Nuncius (Sidereal Messenger), by Galileo, it is possible to trace a methodology to observe the planet and its natural satellites and, with the aid of the astrophotography, to analyze the field recordings. Moreover, it is possible to compare the images obtained after plotting the relative positions of these moons to prove their orbits. This activity can be conducted by teachers, students and amateur astronomers, in order to develop their capabilities of observation of astronomical phenomena. Se presenta en este trabajo una actividad relacionada a la observación de las lunas Galileanas y a la astrofotografía amateur. A través de la lectura de tramos de la obra Sidereus Nuncius (El Mensajero Sideral) de Galileo, es posible trazar una metodología para la observación del planeta y de sus satélites naturales y, con el auxilio de la astrofotografía, analizar las anotaciones de campo. Además, es posible comparar las imágenes obtenidas con el gráfico de las posiciones relativas de estas lunas para comprobar sus órbitas. Esa actividad puede ser realizada por maestros, estudiantes y astrónomos amateurs, de forma a desarrollar sus capacidades de observación de los fenómenos astronómicos. Uma atividade relacionada à observação das luas Galileanas e à astrofotografia amadora é apresentada neste trabalho. Através da leitura de trechos da obra Sidereus Nuncius (Mensageiro Sideral), de Galileu, é possível traçar uma metodologia para a observação do planeta e de seus satélites naturais e, com o auxílio da astrofotografia, analisar as anotações de campo. Além disso, é possível comparar as imagens obtidas com o gráfico das posições relativas destas luas para evidenciar suas órbitas. Essa atividade pode ser realizada por professores, estudantes e astrônomos amadores, de forma a desenvolverem suas capacidades de observar fenômenos astronômicos.

High School Student's Alternative Conceptions About the Phenomenon of the Formation of the Moon Phases. (Spanish Title: Concepciones Alternativas de Alumnos de Educación Media Sobre el Fenómeno de Formación de las Fases de La Luna.) Concepções Alternativas de Alunos do Ensino Médio Sobre o Fenômeno de Formação das Fases da Lua

NASA Astrophysics Data System (ADS)

Iachel, Gustavo; Langhi, Rodolfo; Fernandes Scalvi, Rosa Maria

2008-07-01

Forty students, at ages between 14 and 18 years old, from three schools in Bauru city, were questioned about their alternative conceptions concerning the phenomenon of formation of the Moon Phases. It was observed that some of the pupils confound the phenomenon of the formation of the Moon Phases with the phenomenon of the formation of the lunar eclipses, others are unaware of the reason of the phenomenon, they present incoherent alternative conceptions of the reality or incomplete conceptions. The results found here are aimed at the teachers of Elementary Education and can be used as a subsidy for future development of new pedagogical methods. Cuarenta estudiantes, con edad entre 14 y 18 años, pertenecientes a tres escuelas de la ciudad de Bauru, fueron cuestionados sobre sus concepciones alternativas acerca del fenómeno de la formación de las fases de la Luna. Fue observado que algunos alumnos confunden el fenómeno de formación de las fases de la Luna con el fenómeno de formación de los eclipses lunares, otros desconocen el motivo delfenómeno, presentan concepciones alternativas incoherentes con la realidad o bien presentan concepciones incompletas. Los resultados aquí encontrados son destinados a los profesores de la Enseñanza Básica y podrán ser usados como ayuda para el futuro desarrollo de nuevos métodos pedagógicos. Quarenta estudantes, com idades entre 14 e 18 anos, pertencentes a três escolas da cidade de Bauru, foram questionados sobre suas concepções alternativas acerca do fenômeno de formação das fases da Lua. Foi observado que alguns dos alunos confundem o fenômeno da formação das fases da Lua com o fenômeno da formação dos eclipses lunares, outros desconhecem o motivo do fenômeno, apresentam concepções alternativas incoerentes com a realidade ou então concepções incompletas. Os resultados aqui encontrados são destinados aos professores do Ensino Básico e poderão ser usados como subsídio parafuturo desenvolvimento de novos métodos pedagógicos.

76 FR 20010 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-DVD Copy Control...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-11

... Computer Corp., Taipei, TAIWAN; Dongguan ChuDong Electronic Technology Co., Ltd., Dongguan City, Guangdong... have been made in either the membership or planned activity of the group research project. Membership in this group research project remains open, and DVD CCA intends to file additional written...

A Real-Time Coastal Ocean Prediction Experiment for MREA04

DTIC Science & Technology

2008-01-01

coastal ocean prediction experiment for MREA04 Dong S. Ko *, Paul J. Martin, Clark D. Rowley, Ruth H. Preller Naval Research Laborator ,: S ’ntis Space...Jourml of Marine Svstem 69 t200S) 17 28 and various data streams for ocean bathymetry, clima - global ONFS or from a higher resolution regional ONFS

The Implementation of Standards-Based Teacher Evaluation in Vietnamese Secondary Schools: A Case Study in Dong Thap

ERIC Educational Resources Information Center

Pham, Huy Q.

2013-01-01

Teacher quality has become a critical area of concern in Vietnamese education. Recently, new professional standards for teachers in secondary schools have been developed, piloted, and implemented. This study explores the perceptions of teachers, school principals, and other administrators about the new teacher professional standards, the…

Contribution of Interstitial Deletion of 21q22.2-3 per se to Prostate Cancer Progression in Tumors Harboring TMPRSS2-ERG Translocations

DTIC Science & Technology

2015-12-01

harboring TMPRSS2-ERG translocations PRINCIPAL INVESTIGATOR: Yan Dong CONTRACTING ORGANIZATION: Tulane University New Orleans , LA 70112...AND ADDRESS(ES) Tulane University AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER 6823 Saint Charles Ave New Orleans , LA 70118-5665 9

Australian Institute of Aboriginal Studies Catalogue of Tape Archive No. 6.

ERIC Educational Resources Information Center

Australian Inst. of Aboriginal Studies, Canberra.

The aboriginal studies listed in this catalogue are indexed according to language/tribe and subject (linguistic studies, myths and stories, songs and dances, dongs and music, and speech). Entries are listed alphabetically under the depositor's name. Language/tribe headings are from the Institute's Preliminary Tribal Index. Summaries are brief but…

North Korea’s Nuclear Weapons: Technical Issues

DTIC Science & Technology

2009-07-01

news.bbc.co.uk/2/hi/asia-pacific/6216385.stm. 20 Warren Strobel , “North Korean nuclear documents challenge CIA assertions,” McClatchyNewspapers, May 28, 2008...78 “N. Korea ‘Slowing Disablement of Nuclear Facilities,” Chosun Ilbo, January 29, 2008. 79 Lee Chi-dong, “N Korea Complains

North Korea’s Nuclear Weapons: Technical Issues

DTIC Science & Technology

2009-12-16

News, http://news.bbc.co.uk/2/hi/asia-pacific/6216385.stm. 20 Warren Strobel , “North Korean nuclear documents challenge CIA assertions...Facilities,” Chosun Ilbo, January 29, 2008. 81 Lee Chi-dong, “N Korea Complains About Slow Provision of Energy Aid,” Yonhap News, June 5, 2008. 82 “N

North Korea’s Nuclear Weapons: Technical Issues

DTIC Science & Technology

2009-05-26

Warren Strobel , “North Korean nuclear documents challenge CIA assertions,” McClatchyNewspapers, May 28, 2008. 23 “N.K. says plutonium ‘weaponized’ and...Chosun Ilbo, January 29, 2008. 78 Lee Chi-dong, “N Korea Complains About Slow Provision of Energy Aid,” Yonhap News, June 5, 2008. 79 “N. Korea

North Korea’s Nuclear Weapons

DTIC Science & Technology

2009-05-05

South,” BBC News, http://news.bbc.co.uk/2/hi/asia-pacific/6216385.stm. 22 Warren Strobel , “North Korean nuclear documents challenge CIA assertions...Chosun Ilbo, January 29, 2008. 74 Lee Chi-dong, “N Korea Complains About Slow Provision of Energy Aid,” Yonhap News, June 5, 2008. 75 “N. Korea can

Hyperspectral Imagery for Large Area Survey of Organophosphate Pesticides

DTIC Science & Technology

2015-03-26

possible (Simonian et al., 2004). Nanotechnology has the potential for the development of handheld, real-time, and accurate OP detectors ( Goltz et...dx.doi.org.wrs.idm.oclc.org/10.1016/j.talanta.2012.10.016 Goltz , Mark, N.Dong Shik, Kim, Racz, LeeAnn. (2011). Using nanotechnology to detect nerve agents. Air & Space

The determinants of long-term care utilization and equity of access to care among older adults in Dong-Ku of Incheon Metropolitan city, South Korea.

PubMed

Park, J M

2005-01-01

Under the current health care system, around three percent of the elderly remain uninsured. Based on the 2003 Dong-Ku Health Status Survey and the Aday and Andersen Access Framework, the present study examined the social and behavioral determinants of long-term care utilization and the extent to which equity in the use of long-term care services for the elderly has been achieved. The results indicate that universal health insurance system has not yielded a fully equitable distribution of services. Type of coverage and resource availability do not remain predictors of long-term care utilization. The data suggest that a universal health insurance system exists in South Korea with significant access problems for the population without insurance. Access differences also arise from obstacles in expanding the scope and level of plan benefits due to financial disparity among insurers. Health policy reforms must continue to concentrate on extending insurance coverage to the uninsured and establishing long-term insurance system for the elderly.

First molecular verification of Dixonius vietnamensis Das, 2004 (Squamata: Gekkonidae) with the description of a new species from Vinh Cuu Nature Reserve, Dong Nai Province, Vietnam.

PubMed

Ziegler, Thomas; Botov, Andreas; Nguyen, Tao Thien; Bauer, Aaron M; Brennan, Ian G; Ngo, Hanh Thi; Nguyen, Truong Quang

2016-07-07

Based on near-topotypic specimens of Dixonius vietnamensis from Khanh Hoa Province in southern Vietnam genetic analyses showed that the recently described D. taoi is sister to D. vietnamensis and several separate forms exist which previously have been misidentified as D. vietnamensis and D. siamensis. The Dixonius population from Vinh Cuu Nature Reserve, Dong Nai Province, Vietnam, represents an undescribed species. Dixonius minhlei sp. nov. can be distinguished from its congeners based on the following diagnostic characters: small size (up to 47.5 mm SVL); 7-9 supralabials; 14-15 rows of keeled tubercles on dorsum; 20-23 ventral scale rows; 7 or 8 precloacal pores in males; a canthal stripe running from rostrum through the eye and terminating at back of head; lateral second pair of postmentals maximum one quarter the size of first pair; dorsum olive gray with more or less discernible brownish olive blotches. This is the sixth species of Dixonius known to occur in Vietnam.

Application research for 4D technology in flood forecasting and evaluation

NASA Astrophysics Data System (ADS)

Li, Ziwei; Liu, Yutong; Cao, Hongjie

1998-08-01

In order to monitor the region which disaster flood happened frequently in China, satisfy the great need of province governments for high accuracy monitoring and evaluated data for disaster and improve the efficiency for repelling disaster, under the Ninth Five-year National Key Technologies Programme, the method was researched for flood forecasting and evaluation using satellite and aerial remoted sensed image and land monitor data. The effective and practicable flood forecasting and evaluation system was established and DongTing Lake was selected as the test site. Modern Digital photogrammetry, remote sensing and GIS technology was used in this system, the disastrous flood could be forecasted and loss can be evaluated base on '4D' (DEM -- Digital Elevation Model, DOQ -- Digital OrthophotoQuads, DRG -- Digital Raster Graph, DTI -- Digital Thematic Information) disaster background database. The technology of gathering and establishing method for '4D' disaster environment background database, application technology for flood forecasting and evaluation based on '4D' background data and experimental results for DongTing Lake test site were introduced in detail in this paper.

Effect of “Jian-Pi-Zhi-Dong Decoction” on Gamma-Aminobutyric Acid in a Mouse Model of Tourette Syndrome

PubMed Central

Zhang, Wen; Yu, Wenjing; Wei, Li; Lee, Minkyoung; Wang, Sumei

2014-01-01

The purpose of this study was to explore the positive effects of Jian-Pi-Zhi-Dong Decoction (JPZDD) on Tourette syndrome (TS) by investigating the expression of gamma-aminobutyric acid (GABA) and its type A receptor (GABAAR) in the striatum of a TS mice model. The model was induced by 3,3′-iminodipropionitrile (IDPN) treatment; then mice were divided into 4 groups (n=22, each); control and IDPN groups were gavaged with saline and the remaining 2 groups were gavaged with tiapride and JPZDD. We recorded the stereotypic behaviors of TS mice and measured the content of GABA in striatum by HPLC and GABAAR expression by immunohistochemistry and real-time PCR. Our results showed that JPZDD inhibited the abnormal behaviors of TS model mice and decreased GABA levels and GABAAR protein and mRNA expression in the striatum of TS model mice. In brief, the mechanism by which JPZDD alleviates TS symptoms may be associated with GABAAR expression downregulation in striatum which may regulate GABA metabolism. PMID:24812567

Environmental hazard mapping using GIS and AHP - A case study of Dong Trieu District in Quang Ninh Province, Vietnam

NASA Astrophysics Data System (ADS)

Anh, N. K.; Phonekeo, V.; My, V. C.; Duong, N. D.; Dat, P. T.

2014-02-01

In recent years, Vietnamese economy has been growing up rapidly and caused serious environmental quality plunging, especially in industrial and mining areas. It brings an enormous threat to a socially sustainable development and the health of human beings. Environmental quality assessment and protection are complex and dynamic processes, since it involves spatial information from multi-sector, multi-region and multi-field sources and needs complicated data processing. Therefore, an effective environmental protection information system is needed, in which considerable factors hidden in the complex relationships will become clear and visible. In this paper, the authors present the methodology which was used to generate environmental hazard maps which are applied to the integration of Analytic Hierarchy Process (AHP) and Geographical Information system (GIS). We demonstrate the results that were obtained from the study area in Dong Trieu district. This research study has contributed an overall perspective of environmental quality and identified the devastated areas where the administration urgently needs to establish an appropriate policy to improve and protect the environment.

Biochemical characterization of recombinant Enterovirus 71 3C protease with fluorogenic model peptide substrates and development of a biochemical assay.

PubMed

Shang, Luqing; Zhang, Shumei; Yang, Xi; Sun, Jixue; Li, Linfeng; Cui, Zhengjie; He, Qiuhong; Guo, Yu; Sun, Yuna; Yin, Zheng

2015-04-01

Enterovirus 71 (EV71), a primary pathogen of hand, foot, and mouth disease (HFMD), affects primarily infants and children. Currently, there are no effective drugs against HFMD. EV71 3C protease performs multiple tasks in the viral replication, which makes it an ideal antiviral target. We synthesized a small set of fluorogenic model peptides derived from cleavage sites of EV71 polyprotein and examined their efficiencies of cleavage by EV71 3C protease. The novel peptide P08 [(2-(N-methylamino)benzoyl) (NMA)-IEALFQGPPK(DNP)FR] was determined to be the most efficiently cleaved by EV71 3C protease, with a kinetic constant kcat/Km of 11.8 ± 0.82 mM(-1) min(-1). Compared with literature reports, P08 gave significant improvement in the signal/background ratio, which makes it an attractive substrate for assay development. A Molecular dynamics simulation study elaborated the interactions between substrate P08 and EV71 3C protease. Arg39, which is located at the bottom of the S2 pocket of EV71 3C protease, may participate in the proteolysis process of substrates. With an aim to evaluate EV71 3C protease inhibitors, a reliable and robust biochemical assay with a Z' factor of 0.87 ± 0.05 was developed. A novel compound (compound 3) (50% inhibitory concentration [IC50] = 1.89 ± 0.25 μM) was discovered using this assay, which effectively suppressed the proliferation of EV 71 (strain Fuyang) in rhabdomyosarcoma (RD) cells with a highly selective index (50% effective concentration [EC50] = 4.54 ± 0.51 μM; 50% cytotoxic concentration [CC50] > 100 μM). This fast and efficient assay for lead discovery and optimization provides an ideal platform for anti-EV71 drug development targeting 3C protease. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Internal Wave Study in the South China Sea Using SAR

NASA Technical Reports Server (NTRS)

Liu, Antony K.; Hsu, Ming-Kuang; Zukor, Dorothy (Technical Monitor)

2000-01-01

Recently, the internal wave distribution maps in the China Seas have been compiled from hundreds of ERS-1/2, RADARSAT, and Space Shuttle SAR (Synthetic Aperture Radar) images from 1993 to 1999. Based on internal wave distribution map, most of internal waves in the northeast part of South China Sea were propagating westward. The wave crest can be as long as 200 km with amplitude of 100 m due to strong current from the Kuroshio branching out into the South China Sea. Based on the observations from drilling rigs near DongSha Island by Amoco Production Co., the solitons may be generated in a 4 km wide channel between Batan and Sabtang islands in Luzon Strait. The proposed generation mechanism is similar to the lee wave formation from a shallow topography. Both depression and elevation internal waves have been observed in the same RADARSAT ScanSAR image on May 4, 1998 near DongSha Island. Furthermore, depression and elevation internal waves have also been observed by SAR at the same location on the shelf in April and June, 1993 (in different seasons) respectively. Numerical models have been used to interpret their generation mechanism and evolution processes. Based on the SAR images, near DongSha Island, the westward propagating huge internal solitons are often encountered and diffracted/broken by the coral reefs on the shelf. After passing the island, the diffracted waves will re-merge or interact with each other. It has been observed that after the nonlinear wave-wave interaction, the phase of wave packet is shifted and wavelength is also changed. Examples of mesoscale features observed in SAR images, such as fronts, raincells, bathymetry, ship wakes, and oil spills will be presented. Recent mooring measurements in April 1999 near Dongsha Island, future field test ASIAEX (Asian Seas International Acoustics Experiment) planned for April 2001, and some pretest survey data will be discussed in this paper.

Camp Laguardia, Korea. Limited Surface Observations Climatic Summary (LISOCS). Parts A-F.

DTIC Science & Technology

1988-03-01

OBSERVATIONS" CLIMATIC SUMMARY "LISOCS" CAMP LAGUARDIA KOREA MSC #471060 N 37 44 E 127 03 ELEV 174 FT PKSB PARTS A - F HOURS SUMMARIZED: 0600 - 1800 PERIOD OF...8217.p " .? TOAL C 4 ." TV.’ 0.5 1._ 2. I CTAL N Um’ L’ OF 0 ’ SF ATI ONS ,7 lp i: r L 06 L UC l’±11LOGY YPRtNCH PLPLFA-lbrE FRECUENCY OF OCCUQRCNCE OF...7 uC 1606 25.7 68.3 72 .C 71.b 70.6 72.6 73.9 72.9 7,.9 7’.9 70.9 73.9 7).9 72.9 70.9 GE 14% ’I t5.9 685 %_ 737 7C.7 73.7 71.1 71.1 7.1 71.1 71.1 71.1

26 CFR 1.501(c)(7)-1 - Social clubs.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 26 Internal Revenue 7 2013-04-01 2013-04-01 false Social clubs. 1.501(c)(7)-1 Section 1.501(c)(7... (CONTINUED) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(c)(7)-1 Social clubs. (a) The exemption... general, this exemption extends to social and recreation clubs which are supported solely by membership...

26 CFR 1.501(c)(7)-1 - Social clubs.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 26 Internal Revenue 7 2012-04-01 2012-04-01 false Social clubs. 1.501(c)(7)-1 Section 1.501(c)(7... (CONTINUED) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(c)(7)-1 Social clubs. (a) The exemption... general, this exemption extends to social and recreation clubs which are supported solely by membership...

Developing Xenopus Laevis as a Model to Screen Drugs for Fragile X Syndrome

DTIC Science & Technology

2013-10-01

several candidate treatments for Fragile X Syndrome have gone to clinical trials. Though promising, no treatment has yet been approved. This sad ...Xenopus laevis tadpoles. J Comp Neurol 520, 401-433. Dong, W., Lee, R.H., Xu, H., Yang, S., Pratt, K.G., Cao, V., Song , Y.K., Nurmikko, A., and

Governing "Suzhi" and Curriculum Reform in Rural Ethnic China: Viewpoints from the Miao and Dong Communities in Qiandongnan

ERIC Educational Resources Information Center

Wu, Jinting

2012-01-01

This article examines the uptake of "suzhi"--roughly glossed as "quality"--in China's recent curriculum reform called "suzhi jiaoyu" (Education for Quality) in the rural ethnic context of Qiandongnan. It engages with three layers of analysis. First is a brief etymological overview of "suzhi" to map out its…

Production Methods for a Mesenchymal Stem Cell Therapeutic as a Medical Defense Countermeasure

DTIC Science & Technology

2012-02-01

differentiation of murine embryonic stem cells into vascular progenitors. BMC Cell Biol. 2008;9:2. 56. Johnson EA, Dao TL, Kan RK. Status epilepticus ...their undifferentiated and multipotent status . BMC Cell Biol. 2011;12:12. 52. Sun Y, Chen L, Hou XG, Hou WK, Dong JJ, Sun L, et al. Differentiation of

Korean Confucianism's Mindful Learning Model of Moral Internalization as Manifested in the Cheonghak-Dong Hermit Community

ERIC Educational Resources Information Center

Yoo, Si Ha

2012-01-01

The purpose of this qualitative study is to understand an unknown educational approach in Korean Confucianism's ethical education characterized as moral internalization, based on the study participants. A prevailed understanding of moral internalization of Confucianism is a strong imposed learning process, which is a well known Asian learning…

NONYLPHENOL AND ATRAZINE INDUCE INVERSE EFFECTS ON MAMMARY GLAND DEVELOPMENT IN FEMALE RATS EXPOSED IN UTERO

EPA Science Inventory

Nonylphenol and Atrazine Induce Inverse Effects on Mammary Gland Development in Female Rats Exposed In Utero.
HJ Moon1, SY Han1, CC Davis2, and SE Fenton2
1 Department of Toxicology, NITR, Korea FDA, 5Nokbun-Dong, Eunpyung-Gu, Seoul, Korea and 2 Reproductive Toxicology Divi...

Reemergence of enterovirus 71 epidemic in northern Taiwan, 2012.

PubMed

Luo, Shu-Ting; Chiang, Pai-Shan; Chung, Wan-Yu; Chia, Min-Yuan; Tsao, Kuo-Chien; Wang, Ying-Hsiang; Lin, Tzou-Yien; Lee, Min-Shi

2015-01-01

Enterovirus 71 (EV71) belongs to picornavirus family and could be classified phylogenetically into three major genogroups (A, B and C) including 11 genotypes (A, B1-B5 and C1-C5). Since 1997, EV71 has caused large-scale of epidemics with neurological complications in Asian children. In Taiwan, nationwide EV71 epidemics with different predominant genotypes have occurred cyclically since 1998. A nationwide EV71 epidemic occurred again in 2012. We conducted genetic and antigenic characterizations of the 2012 epidemic. Chang Gung Memorial Hospital (CGMH) is a medical center in northern Taiwan. In CGMH, specimens were collected from pediatric inpatients with suspected enterovirus infections for virus isolation. Enterovirus isolates were serotyped and genotyped and sera from EV71 inpatients were collected for measuring neutralizing antibody titers. There were 10, 16 and 99 EV71 inpatients identified in 2010, 2011 and 2012, respectively. There were 82 EV71 isolates genotyped, which identified 17 genotype C4a viruses and 65 genotype B5 viruses. The genotype B5 viruses were not detected until November 2011 and caused epidemics in 2012. Interestingly, the B5-2011 viruses were genetically distinguishable from the B5 viruses causing the 2008 epidemic and are likely introduced from China or Southeastern Asia. Based on antigenic analysis, minor antigenic variations were detected among the B5-2008, B5-2011, C4a-2008 and C4a-2012 viruses but these viruses antigenically differed from genotype A. Genotype B5 and C4a viruses antigenically differ from genotype A viruses which have disappeared globally for 30 years but have been detected in China since 2008. Enterovirus surveillance should monitor genetic and antigenic variations of EV71.

75 FR 20387 - Amended Certification Regarding Eligibility To Apply for Worker Adjustment Assistance

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-19

..., Meadville, Pennsylvania. TA-W-71,272C, Crucible Materials Corporation, Crucible Service Center, Troy..., Pennsylvania; Troy, Michigan; Butler, Wisconsin; Miamisburg, Ohio; Chicago, Illinois; Minneapolis, Minnesota...); Troy, Michigan (TA-W-71,272C); Butler, Wisconsin (TA-W-71,272D); Miamisburg, Ohio (TA-W-71,272E...

Co-Circulation and Genomic Recombination of Coxsackievirus A16 and Enterovirus 71 during a Large Outbreak of Hand, Foot, and Mouth Disease in Central China

PubMed Central

Liu, Weiyong; Wu, Shimin; Xiong, Ying; Li, Tongya; Wen, Zhou; Yan, Mingzhe; Qin, Kai; Liu, Yingle; Wu, Jianguo

2014-01-01

A total of 1844 patients with hand, foot, and mouth disease (HFMD), most of them were children of age 1–3-year-old, in Central China were hospitalized from 2011 to 2012. Among them, 422 were infected with coxsackievirus A16 (CVA16), 334 were infected with enterovirus 71 (EV71), 38 were co-infected with EV71 and CVA16, and 35 were infected with other enteroviruses. Molecular epidemiology analysis revealed that EV71 and CVA16 were detected year-round, but EV71 circulated mainly in July and CVA16 circulated predominantly in November, and incidence of HFMD was reduced in January and February and increased in March. Clinical data showed that hyperglycemia and neurologic complications were significantly higher in EV71-infected patients, while upper respiratory tract infection and C-reactive protein were significantly higher in CVA16-associated patients. 124 EV71 and 80 CVA16 strains were isolated, among them 56 and 68 EV71 strains were C4a and C4b, while 25 and 55 CVA16 strains were B1a and B1b, respectively. Similarity plots and bootscan analyses based on entire genomic sequences revealed that the three C4a sub-genotype EV71 strains were recombinant with C4b sub-genotype EV71 in 2B–2C region, and the three CVA16 strains were recombinant with EV71 in 2A–2B region. Thus, CVA16 and EV71 were the major causative agents in a large HFMD outbreak in Central China. HFMD incidence was high for children among household contact and was detected year-round, but outbreak was seasonal dependent. CVA16 B1b and EV71 C4b reemerged and caused a large epidemic in China after a quiet period of many years. Moreover, EV71 and CVA16 were co-circulated during the outbreak, which may have contributed to the genomic recombination between the pathogens. It should gain more attention as there may be an upward trend in co-circulation of the two pathogens globally and the new role recombination plays in the emergence of new enterovirus variants. PMID:24776922

Changes in urinary Cu, Zn, and Se levels in cancer patients after treatment with Sha Shen Mai Men Dong Tang

PubMed Central

Lai, Tung-Yuan; Kuo, Hsien-Wen

2015-01-01

Sha Shen Mai Men Dong Tang (SMD-2; 沙參麥冬湯 shā shēn mài dōng tāng) is a Chinese medicinal herb (CMH; 中草藥 zhōng cǎo yào) used to treat symptoms associated with cancer therapy. The objective of this study was to assess the effect of SMD-2 on the levels of urinary copper (Cu), zinc (Zn), and selenium (Se) in lung cancer patients and head and neck cancer patients receiving chemoradiotherapy. Forty-two head and neck cancer patients and 10 lung cancer patients participated in our clinical trial. Each patient received chemoradiotherapy for 4 weeks. In addition, each patient was treated with SMD-2 for 8 weeks, including 2 weeks prior to and after the chemoradiotherapy treatment. Comparison of urinary Cu, Zn, and Se levels and the ratios of Zn to Cu and Se to Cu at three time points in the two types of cancer were assessed using the generalized estimating equations (GEEs). After the patients received chemoradiotherapy for 4 weeks, SMD-2 treatment was found to be associated with a significant decrease in urinary Cu levels, whereas urinary Zn and Se levels increased significantly. In addition, the ratios of Zn to Cu and Se to Cu in the urine samples of these patients also increased significantly. Both the urinary Zn levels and the ratio of Zn to Cu in head and neck cancer patients were significantly higher than in lung cancer patients. Urinary Zn and Se levels and the ratios of Zn to Cu and Se to Cu, but not urinary Cu levels, increased significantly during and after treatment when assessed using the GEE model. The SMD-2 treatments significantly increased Zn and Se levels in the urine of head and neck cancer patients. Increased Zn and Se levels in urine strengthened immune system. PMID:27114935

Spiribacter curvatus sp. nov., a moderately halophilic bacterium isolated from a saltern.

PubMed

León, María José; Rodríguez-Olmos, Angel; Sánchez-Porro, Cristina; López-Pérez, Mario; Rodríguez-Valera, Francisco; Soliveri, Juan; Ventosa, Antonio; Copa-Patiño, José Luis

2015-12-01

A novel pink-pigmented bacterial strain, UAH-SP71T, was isolated from a saltern located in Santa Pola, Alicante (Spain) and the complete genome sequence was analysed and compared with that of Spiribacter salinus M19-40T, suggesting that the two strains constituted two separate species, with a 77.3% ANI value. In this paper, strain UAH-SP71T was investigated in a taxonomic study using a polyphasic approach. Strain UAH-SP71T was a Gram-stain-negative, strictly aerobic, non-motile curved rod that grew in media containing 5-20% (w/v) NaCl (optimum 10% NaCl), at 5-40 °C (optimum 37 °C) and at pH 5-10 (optimum pH 8). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed thatstrain UAH-SP71T is a member of the genus Spiribacter, showing a sequence similarity of 96.5% with Spiribacter salinus M19-40T. Other related species are also members of the family Ectothiorhodospiraceae, including Arhodomonas recens RS91T (95.5% 16S rRNA gene sequence similarity), Arhodomonas aquaeolei ATCC 49307T (95.4 %) and Alkalilimnicola ehrlichii MLHE-1T (94.9 %). DNA-DNA hybridization between strain UAH-SP71T and Spiribacter salinus M19-40T was 39 %. The major cellular fatty acids of strain UAH-SP71T were C18 : 1ω6c and/or C18 : 1ω7c, C16 : 0, C16 : 1ω6c and/or C16 : 1ω7c, C10 : 0 3-OH and C12 : 0, a pattern similar to that of Spiribacter salinus M19-40T. Phylogenetic, phenotypic and genotypic differences between strain UAH-SP71T and Spiribacter salinus M19-40T indicate that strainUAH-SP71T represents a novel species of the genus Spiribacter, for which the name Spiribacter curvatus sp. nov. is proposed. The type strain is UAH-SP71T (5CECT8396T5DSM 28542T).

Enterovirus-71 genotype C isolated in Peru between 2006 and 2009.

PubMed

Huaman, Jose L; Carrion, Gladys; Ampuero, Julia S; Ocaña, Victor; Laguna-Torres, V Alberto; Hontz, Robert D

2016-12-01

Enterovirus-71 (EV71) was first isolated in California, United States in 1969, belongs to the genus Enterovirus, family Picornaviridae. Although infection normally causes mild, often undiagnosed illness, it can cause central nervous system infections that could turn fatal. Based on VP1 gene analysis, EV71 has been classified into six separate genotypes. Although the molecular epidemiology of EV71 has been well described via studies originating from Asia and Europe, it is mostly unknown in South America. From our study, four EV71 isolates from Peru were characterized using phylogenetic methods to determine their relationship with known reference strains. These four Peruvian EV71 isolates from between 2006 and 2009 were analyzed by RT-PCR using primers capable of amplifying the entire VP1 gene. Reference strains representing all six known genotypes were used to determine any recognizable phylogenetic relationships. In fact, all of our isolates clustered together within the genotype C1 lineage- separate from Asian, European, North American, and Australian strains. We present evidence that EV71 genotype C1 exists in Peru, and this is the first such report documenting EV71 genotype C1 circulating in South America. Gathering additional isolates will help elucidate a more complete global epidemiological picture of EV71 infections. Published by Elsevier B.V.

Enterovirus 71 2C Protein Inhibits NF-κB Activation by Binding to RelA(p65)

PubMed Central

Du, Haiwei; Yin, Peiqi; Yang, Xiaojie; Zhang, Leiliang; Jin, Qi; Zhu, Guofeng

2015-01-01

Viruses evolve multiple ways to interfere with NF-κB signaling, a key regulator of innate and adaptive immunity. Enterovirus 71 (EV71) is one of primary pathogens that cause hand-foot-mouth disease. Here, we identify RelA(p65) as a novel binding partner for EV71 2C protein from yeast two-hybrid screen. By interaction with IPT domain of p65, 2C reduces the formation of heterodimer p65/p50, the predominant form of NF-κB. We also show that picornavirus 2C family proteins inhibit NF-κB activation and associate with p65 and IKKβ. Our findings provide a novel mechanism how EV71 antagonizes innate immunity. PMID:26394554

Highly Conserved Keratin-Associated Protein 7-1 Gene in Yak, Taurine and Zebu Cattle.

PubMed

Arlud, S; He, N; Sari, E M; Ma, Z-J; Zhang, H; An, T-W; Han, J-L

2017-01-01

Keratin-associated proteins (KRTAPs) play a critical role in cross-linking the keratin intermediate filaments to build a hair shaft. The genetic polymorphisms of the bovine KRTAP7-1 gene were investigated for the first time in this study. The complete coding sequence of the KRTAP7-1 gene in 108 domestic yak, taurine and zebu cattle from China and Indonesia were successfully amplified using polymerase chain reaction and then directly sequenced. Only two single-nucleotide polymorphisms (one nonsynonymous at c.7C/G and another synonymous at c.21C/T) and three haplotypes (BOVIN-KRTAP7-1*A, B and C) were identified in the complete coding sequence of the bovine KRTAP7-1 gene among all animals. There was no polymorphism across three Chinese indigenous yak breeds and one Indonesian zebu cattle population, all sharing the BOVINKRTAP71*A haplotype. The four taurine cattle populations also had BOVIN-KRTAP7-1*A as the most common haplotype with a frequency of 0.80. The frequency of novel haplotype BOVIN-KRTAP7-1*B was only 0.07 present in one heterozygous animal in each of the four taurine cattle populations, while BOVINKRTAP7- 1*C was only found in a Simmental and a local Chinese Yellow cattle population with frequencies of 0.17 and 0.36, respectively. The monomorphic yak KRTAP7-1 gene in particular, and highly conserved bovine, sheep and goat KRTAP7-1 genes in general, demonstrated its unique intrinsic structural property (e.g., > 21% high glycine content) and primary functional importance in supporting the mechanical strength and shape of hair.

Sparsity Aware Adaptive Radar Sensor Imaging in Complex Scattering Environments

DTIC Science & Technology

2015-06-15

while meeting the requirement on the peak to average power ratio. Third, we study impact of waveform encoding on nonlinear electromagnetic tomographic...Enyue Lu. Time Domain Electromagnetic Tomography Using Propagation and Backpropagation Method, IEEE International Conference on Image Processing...Received Paper 3.00 4.00 Yuanwei Jin, Chengdon Dong, Enyue Lu. Waveform Encoding for Nonlinear Electromagnetic Tomographic Imaging, IEEE Global

A Canon, a Norm and an Attitude--Ideological Dimensions in Foreign Language Education in China.

ERIC Educational Resources Information Center

Feng, Anwei

An examination of China's policy, "making the ancient serve the present and foreign things serve China," introduced by Mao Ze-dong, is intended to provoke further discussion of the ideological dimension of foreign language education (FLE) in China. Some observers suggest that this has been an important guiding doctrine in FLE since 1949,…

More Chinese Students Abroad Are Deciding Not To Return Home.

ERIC Educational Resources Information Center

Hertling, James

1997-01-01

In 18 years, over 260,000 Chinese students have left China to study abroad, and only about one-third have returned. Their flight is compounding the devastation of China's knowledge and talent pool that began with Mao Ze-dong. China is encouraging study abroad, to rectify the loss of a generation of academics, and is most interested in science and…

Enhancing the Predictive Power of Mutations in the C-Terminus of the KCNQ1-Encoded Kv7.1 Voltage-Gated Potassium Channel.

PubMed

Kapplinger, Jamie D; Tseng, Andrew S; Salisbury, Benjamin A; Tester, David J; Callis, Thomas E; Alders, Marielle; Wilde, Arthur A M; Ackerman, Michael J

2015-04-01

Despite the overrepresentation of Kv7.1 mutations among patients with a robust diagnosis of long QT syndrome (LQTS), a background rate of innocuous Kv7.1 missense variants observed in healthy controls creates ambiguity in the interpretation of LQTS genetic test results. A recent study showed that the probability of pathogenicity for rare missense mutations depends in part on the topological location of the variant in Kv7.1's various structure-function domains. Since the Kv7.1's C-terminus accounts for nearly 50 % of the overall protein and nearly 50 % of the overall background rate of rare variants falls within the C-terminus, further enhancement in mutation calling may provide guidance in distinguishing pathogenic long QT syndrome type 1 (LQT1)-causing mutations from rare non-disease-causing variants in the Kv7.1's C-terminus. Therefore, we have used conservation analysis and a large case-control study to generate topology-based estimative predictive values to aid in interpretation, identifying three regions of high conservation within the Kv7.1's C-terminus which have a high probability of LQT1 pathogenicity.

Hexon-modified recombinant E1-deleted adenoviral vectors as bivalent vaccine carriers for Coxsackievirus A16 and Enterovirus 71.

PubMed

Zhang, Chao; Yang, Yong; Chi, Yudan; Yin, Jieyun; Yan, Lijun; Ku, Zhiqiang; Liu, Qingwei; Huang, Zhong; Zhou, Dongming

2015-09-22

Hand, foot and mouth disease (HFMD) is a major public health concern in Asia; more efficient vaccines against HFMD are urgently required. Adenoviral (Ad) capsids have been used widely for the presentation of foreign antigens to induce specific immune responses in the host. Here, we describe a novel bivalent vaccine for HFMD based on the hexon-modified, E1-deleted chimpanzee adenovirus serotype 68 (AdC68). The novel vaccine candidate was generated by incorporating the neutralising epitope of Coxsackievirus A16 (CA16), PEP71, into hypervariable region 1 (HVR1), and a shortened neutralising epitope of Enterovirus 71 (EV71), sSP70, into HVR2 of the AdC68 hexon. In order to enhance the immunogenicity of EV71, VP1 of EV71 was cloned into the E1-region of the AdC68 vectors. The results demonstrated that these two epitopes were well presented on the virion surface and had high affinity towards specific antibodies, and VP1 of EV71 was also significantly expressed. In pre-clinical mouse models, the hexon-modified AdC68 elicited neutralising antibodies against both CA16 and EV71, which conferred protection to suckling mice against a lethal challenge of CA16 and EV71. In summary, this study demonstrates that the hexon-modified AdC68 may represent a promising bivalent vaccine carrier against EV71 and CA16 and an epitope-display platform for other pathogens. Copyright © 2015 Elsevier Ltd. All rights reserved.

RDTC [Restricted Data Transmission Controller] global variable definitions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grambihler, A.J.; O`Callaghan, P.B.

The purpose of the Restricted Data Transmission Controller (RDTC) is to demonstrate a methodology for transmitting data between computers which have different levels of classification. The RDTC does this by logically filtering the data being transmitted between the two computers. This prototype is set up to filter data from the classified computer so that only numeric data is passed to the unclassified computer. The RDTC allows all data from the unclassified computer to be sent to the classified computer. The classified system is referred to as LUA and the unclassified system is referred to as LUB. 9 tabs.

Impaired Auditory and Contextual Fear Conditioning in Soman-Exposed Rats

DTIC Science & Technology

2011-01-01

include the piriform cortex, amygdala, thalamus and hippocampus (Carpentier et al., 1990; Petras , 1994; Shih et al., 2003). Often the resulting... Martin M, Shah R, Bertchume A, Colvin J, Dong H. Cholinesterase inhibitors ameliorate behavioral deficits induced by MK-801 in mice. Neuropsy...Csernansky CA, Martin MV, Bertchume A, Vallera D, Csernansky JG. Acetylcholinesterase inhibitors ameliorate behavioral deficits in the Tg2576 mouse

Ding Dong, You've Got Mail! A Lab Activity for Teaching the Internet of Things

ERIC Educational Resources Information Center

Frydenberg, Mark

2017-01-01

Connecting ordinary devices to the Internet is a defining characteristic of the Internet of Things. In this hands-on lab activity, students will connect a wireless doorbell to the Internet using a Raspberry Pi computer. By modifying and running a program on the Raspberry Pi to send an email or text message notifying a recipient that someone is at…

Symposium LL: Nanowires--Synthesis Properties Assembly and Application

DTIC Science & Technology

2010-09-10

dedicated hard x - ray microscopy beamline is operated in partnership with the Advanced Photon Source to provide fluorescence, diffraction, and...characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X - ray diffraction (XRD) measurements, proving it to be...Investigation of Preferred Growth Direction of GaN Nanorods by Synchrotron X - ray Reciprocal Space Mapping. Yuri Sohn1, Sanghwa Lee1, Chinkyo Kim1 and Dong

Describing the primary care system capacity for the prevention and management of non-communicable diseases in rural Vietnam.

PubMed

Van Minh, Hoang; Do, Young Kyung; Bautista, Mary Ann Cruz; Tuan Anh, Tran

2014-01-01

The primary care system in Vietnam has been shown to play a crucial role in disease prevention and health promotion. This study described the primary care system in a selected rural area in Vietnam in terms of its capacity for prevention and control of non-communicable diseases (NCDs). The study was conducted in 2011 in Dong Hy district, Thai Nguyen province-a rural community located in northern Vietnam. Mixed methods were used, including quantitative and qualitative and literature review approaches, to collect data on the current status of the six building blocks of the primary care system in Dong Hy district. Selected health workers and stakeholders in the selected healthcare facilities were surveyed. A description of Dong Hy district's primary care capacity for NCD prevention and control is reported. (i) Service delivery: The current practice in NCD prevention and treatment is mainly based on a single risk factor rather than a combination of cardiovascular disease risks. (ii) Governance: At the primary care level, multi-sectoral collaborations are limited, and there is insufficient integration of NCD preventive activities. (iii) Financing: A national budget for NCD prevention and control is lacking. The cost of treatment and medicines is high, whereas the health insurance scheme limits the list of available medicines and the reimbursement ceiling level. Health workers have low remuneration despite their important roles in NCD prevention. (iv) Human resources: The quantity and quality of health staff working at the primary care level, especially those in preventive medicine, are insufficient. (v) Information and research: The health information system in the district is weak, and there is no specific information system for collecting population-based NCD data. (vi) Medical products and technology: Not all essential equipment and medicines recommended by the WHO are always available at the commune health centre. The capacity of the primary care system in Vietnam is still inadequate to serve the NCD-related health needs of the population. There is an urgent need to improve the primary care capacity for NCD prevention and management in Vietnam. Copyright © 2013 John Wiley & Sons, Ltd.

[DONG's extraordinary acupoints for the ovarian function of polycystic ovary syndrome:a randomized controlled pilot trial].

PubMed

Cao, Yu; Zhang, Li; Zhao, Danyang; Liu, Zhishun

2017-07-12

To preliminary assess the efficacy of DONG 's extraordinary acupoints for the ovarian function of polycystic ovary syndrome. Fifty-six patients were randomized into an acupuncture group and a western medication group. In the acupuncture group, the acupoints were Fuke, Huanchao, Tianhuang (Yinlingquan, SP 9), Renhuang (Sanyinjiao, SP 6), Guanyuan (CV 4), Zigong (EX-CA 1). The treatment was used twice a week for continuous 12 weeks. In the western medication group, patients were given diane-35 for 3 menstrual cycles, once a day for 21 days with 7 days between the two sessions. The ratio of luteinizing hormone/flitropin (LH/FSH) was taken as the primary index, while the secondary indices were serum testosterone (T), FSH, LH, body mass index (BMI), the condition of ovary and menstruation. ①After treatment, the ratio of LH/FSH declined in both the two groups ( P <0.05, P <0.01). The decreasing values of the two groups were not significantly different ( P >0.05). ②T, LH and BMI dropped in the two groups ( P <0.05, P <0.01). The decreasing values of the three indices between the two groups were not significantly different (all P >0.05). ③The size of ovary did not change significantly after treatment in the two groups (both P >0.05), but the reduction degree of ovary size of the acupuncture group was larger than that of the western medication group ( P <0.05). The ovary number of more than 10 foliiculi in single ovary in the acupuncture group reduced compared with that before treatment ( P <0.05). The reduction degree of ovary number with the above feature was not significantly different between the two groups ( P >0.05). ④The times and days of menstruation within 3 months increased significantly in the two groups (all P <0.01), without significance of different valves between the two groups (both P >0.05). DONG 's extraordinary acupoints could improve the ovarian function of polycystic ovary syndrome, whose effect is similar as diane-35.

Characterization of the R162W Kir7.1 mutation associated with snowflake vitreoretinopathy

PubMed Central

Zhang, Wei; Zhang, Xiaoming; Wang, Hui; Sharma, Anil K.; Edwards, Albert O.

2013-01-01

KCNJ13 encodes Kir7.1, an inwardly rectifying K+ channel that is expressed in multiple ion-transporting epithelia. A mutation in KCNJ13 resulting in an arginine-to-tryptophan change at residue 162 (R162W) of Kir7.1 was associated with snowflake vitreoretinal degeneration, an inherited autosomal-dominant disease characterized by vitreous degeneration and mild retinal degeneration. We used the Xenopus laevis oocyte expression system to assess the functional properties of the R162W (mutant) Kir7.1 channel and determine how wild-type (WT) Kir7.1 is affected by the presence of the mutant subunit. Recordings obtained via the two-electrode voltage-clamp technique revealed that injection of oocytes with mutant Kir7.1 cRNA resulted in currents and cation selectivity that were indistinguishable from those in water-injected oocytes, suggesting that the mutant protein does not form functional channels in the plasma membrane. Coinjection of oocytes with equal amounts of mutant and WT Kir7.1 cRNAs resulted in inward K+ and Rb+ currents with amplitudes that were ∼17% of those in oocytes injected with WT Kir7.1 cRNA alone, demonstrating a dominant-negative effect of the mutant subunit. Similar to oocytes injected with WT Kir7.1 cRNA alone, coinjected oocytes exhibited inwardly rectifying Rb+ currents that were more than seven times larger than K+ currents, indicating that mutant subunits did not alter Kir7.1 channel selectivity. Immunostaining of Xenopus oocytes or Madin-Darby canine kidney cells expressing mutant or WT Kir7.1 demonstrated distribution of both proteins primarily in the plasma membrane. Our data suggest that the R162W mutation suppresses Kir7.1 channel activity, possibly by negatively impacting gating by membrane phosphadidylinositol 4,5-bisphosphate. PMID:23255580

Vacuum ultraviolet photoionization of carbohydrates and nucleotides

NASA Astrophysics Data System (ADS)

Shin, Joong-Won; Bernstein, Elliot R.

2014-01-01

Carbohydrates (2-deoxyribose, ribose, and xylose) and nucleotides (adenosine-, cytidine-, guanosine-, and uridine-5'-monophosphate) are generated in the gas phase, and ionized with vacuum ultraviolet photons (VUV, 118.2 nm). The observed time of flight mass spectra of the carbohydrate fragmentation are similar to those observed [J.-W. Shin, F. Dong, M. Grisham, J. J. Rocca, and E. R. Bernstein, Chem. Phys. Lett. 506, 161 (2011)] for 46.9 nm photon ionization, but with more intensity in higher mass fragment ions. The tendency of carbohydrate ions to fragment extensively following ionization seemingly suggests that nucleic acids might undergo radiation damage as a result of carbohydrate, rather than nucleobase fragmentation. VUV photoionization of nucleotides (monophosphate-carbohydrate-nucleobase), however, shows that the carbohydrate-nucleobase bond is the primary fragmentation site for these species. Density functional theory (DFT) calculations indicate that the removed carbohydrate electrons by the 118.2 nm photons are associated with endocyclic C-C and C-O ring centered orbitals: loss of electron density in the ring bonds of the nascent ion can thus account for the observed fragmentation patterns following carbohydrate ionization. DFT calculations also indicate that electrons removed from nucleotides under these same conditions are associated with orbitals involved with the nucleobase-saccharide linkage electron density. The calculations give a general mechanism and explanation of the experimental results.

Vacuum ultraviolet photoionization of carbohydrates and nucleotides.

PubMed

Shin, Joong-Won; Bernstein, Elliot R

2014-01-28

Carbohydrates (2-deoxyribose, ribose, and xylose) and nucleotides (adenosine-, cytidine-, guanosine-, and uridine-5(')-monophosphate) are generated in the gas phase, and ionized with vacuum ultraviolet photons (VUV, 118.2 nm). The observed time of flight mass spectra of the carbohydrate fragmentation are similar to those observed [J.-W. Shin, F. Dong, M. Grisham, J. J. Rocca, and E. R. Bernstein, Chem. Phys. Lett. 506, 161 (2011)] for 46.9 nm photon ionization, but with more intensity in higher mass fragment ions. The tendency of carbohydrate ions to fragment extensively following ionization seemingly suggests that nucleic acids might undergo radiation damage as a result of carbohydrate, rather than nucleobase fragmentation. VUV photoionization of nucleotides (monophosphate-carbohydrate-nucleobase), however, shows that the carbohydrate-nucleobase bond is the primary fragmentation site for these species. Density functional theory (DFT) calculations indicate that the removed carbohydrate electrons by the 118.2 nm photons are associated with endocyclic C-C and C-O ring centered orbitals: loss of electron density in the ring bonds of the nascent ion can thus account for the observed fragmentation patterns following carbohydrate ionization. DFT calculations also indicate that electrons removed from nucleotides under these same conditions are associated with orbitals involved with the nucleobase-saccharide linkage electron density. The calculations give a general mechanism and explanation of the experimental results.

The Current Status of the Disease Caused by Enterovirus 71 Infections: Epidemiology, Pathogenesis, Molecular Epidemiology, and Vaccine Development.

PubMed

Chang, Ping-Chin; Chen, Shou-Chien; Chen, Kow-Tong

2016-09-09

Enterovirus 71 (EV71) infections have a major public health impact in the Asia-Pacific region. We reviewed the epidemiology, pathogenesis, and molecular epidemiology of EV71 infection as well as EV71 vaccine development. Previous studies were found using the search terms "enterovirus 71" and "epidemiology" or "pathogenesis" or "molecular epidemiology" or "vaccine" in Medline and PubMed. Articles that were not published in the English language, manuscripts without an abstract, and opinion articles were excluded from the review. The reported epidemiology of cases caused by EV71 infection varied from country to country; seasonal variations in incidence were observed. Most cases of EV71 infection that resulted in hospitalization for complications occurred in children less than five years old. The brainstem was the most likely major target of EV71 infection. The emergence of the EV71 epidemic in the Asia-Pacific region has been associated with the circulation of different genetic lineages (genotypes B3, B4, C1, C2, and C4) that appear to be undergoing rapid evolutionary changes. The relationship between the gene structure of the EV71 virus and the factors that ensure its survival, circulation, and evasion of immunity is still unknown. EV71 infection has emerged as an important global public health problem. Vaccine development, including the development of inactivated whole-virus live attenuated, subviral particles, and DNA vaccines, has been progressing.

The Current Status of the Disease Caused by Enterovirus 71 Infections: Epidemiology, Pathogenesis, Molecular Epidemiology, and Vaccine Development

PubMed Central

Chang, Ping-Chin; Chen, Shou-Chien; Chen, Kow-Tong

2016-01-01

Enterovirus 71 (EV71) infections have a major public health impact in the Asia-Pacific region. We reviewed the epidemiology, pathogenesis, and molecular epidemiology of EV71 infection as well as EV71 vaccine development. Previous studies were found using the search terms “enterovirus 71” and “epidemiology” or “pathogenesis” or “molecular epidemiology” or “vaccine” in Medline and PubMed. Articles that were not published in the English language, manuscripts without an abstract, and opinion articles were excluded from the review. The reported epidemiology of cases caused by EV71 infection varied from country to country; seasonal variations in incidence were observed. Most cases of EV71 infection that resulted in hospitalization for complications occurred in children less than five years old. The brainstem was the most likely major target of EV71 infection. The emergence of the EV71 epidemic in the Asia-Pacific region has been associated with the circulation of different genetic lineages (genotypes B3, B4, C1, C2, and C4) that appear to be undergoing rapid evolutionary changes. The relationship between the gene structure of the EV71 virus and the factors that ensure its survival, circulation, and evasion of immunity is still unknown. EV71 infection has emerged as an important global public health problem. Vaccine development, including the development of inactivated whole-virus live attenuated, subviral particles, and DNA vaccines, has been progressing. PMID:27618078

Chapter 1.1 Process Scale-Up of Cellulose Nanocrystal Production to 25 kg per Batch at the Forest Products Laboratory

Treesearch

Richard S. Reiner; Alan W. Rudie

2013-01-01

The Fiber and Chemical Sciences Research Work Unit at the Forest Products Laboratory began working out the preparation of cellulose nanocrystals in 2006, using the method of Dong, Revol, and Gray. Initial samples were provided to several scientists within the Forest Service. Continued requests for this material forced scale-up from the initial 20 g scale to kg...

Quantum Algorithms Based on Physical Processes

DTIC Science & Technology

2013-12-03

quantum walks with hard-core bosons and the graph isomorphism problem,” American Physical Society March meeting, March 2011 Kenneth Rudinger, John...King Gamble, Mark Wellons, Mark Friesen, Dong Zhou, Eric Bach, Robert Joynt, and S.N. Coppersmith, “Quantum random walks of non-interacting bosons on...and noninteracting Bosons to distinguish nonisomorphic graphs. 1) We showed that quantum walks of two hard-core Bosons can distinguish all pairs of

Quantum Algorithms Based on Physical Processes

DTIC Science & Technology

2013-12-02

quantum walks with hard-core bosons and the graph isomorphism problem,” American Physical Society March meeting, March 2011 Kenneth Rudinger, John...King Gamble, Mark Wellons, Mark Friesen, Dong Zhou, Eric Bach, Robert Joynt, and S.N. Coppersmith, “Quantum random walks of non-interacting bosons on...and noninteracting Bosons to distinguish nonisomorphic graphs. 1) We showed that quantum walks of two hard-core Bosons can distinguish all pairs of

Probing High School Students' Cognitive Structures and Key Areas of Learning Difficulties on Ethanoic Acid Using the Flow Map Method

ERIC Educational Resources Information Center

Zhou, Qing; Wang, Tingting; Zheng, Qi

2015-01-01

The purpose of this study was primarily to explore high school students' cognitive structures and to identify their learning difficulties on ethanoic acid through the flow map method. The subjects of this study were 30 grade 1 students from Dong Yuan Road Senior High School in Xi'an, China. The interviews were conducted a week after the students…

Educational and Social Development in Doi Ang Khang: A Comparative Study of the Khob Dong and Nor Lae Villages

ERIC Educational Resources Information Center

Frost, Timothy; Gray, Lauren; Lake, John; Roy, Jessica

2007-01-01

Northern Thailand is home to many hill tribes, who have lived for hundreds of years as subsistence farmers or nomadic hunters. In the 1940s, communist insurgencies drove many hill tribes from China, Burma, and other nations south into northern Thailand. Uprooted from their native lands, the hill tribes left behind ties to economic resources and,…

Whole-genome sequence of Clostridium lituseburense L74, isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus.

PubMed

Lee, Yookyung; Lim, Sooyeon; Rhee, Moon-Soo; Chang, Dong-Ho; Kim, Byoung-Chan

2016-03-01

Clostridium lituseburense L74 was isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus collected in Yeong-dong, Chuncheongbuk-do, South Korea and subjected to whole genome sequencing on HiSeq platform and annotated on RAST. The nucleotide sequence of this genome was deposited into DDBJ/EMBL/GenBank under the accession NZ_LITJ00000000.

Trauma is Danger

DTIC Science & Technology

2011-01-01

self interactions and thereby neglect most traumatic injuries , the Danger model abandons this classical concept [ 2 ]. The Danger model theorizes that...June 2011 References 1. Keel M, Trentz O: Pathophysiology of polytrauma. Injury 2005, 36:691-709. 2 . Matzinger P: Tolerance, danger, and the extended... ischemia - reperfusion . J Exp Med 2005, 201:1135-1143. 27. Huang LF, Yao YM, Zhang LT, Dong N, Yu Y, Sheng ZY: The effect of high- mobility group box 1

Where "Sign Language Studies" Has Led Us in Forty Years: Opening High School and University Education for Deaf People in Viet Nam through Sign Language Analysis, Teaching, and Interpretation

ERIC Educational Resources Information Center

Woodward, James; Hoa, Nguyen Thi

2012-01-01

This paper discusses how the Nippon Foundation-funded project "Opening University Education to Deaf People in Viet Nam through Sign Language Analysis, Teaching, and Interpretation," also known as the Dong Nai Deaf Education Project, has been implemented through sign language studies from 2000 through 2012. This project has provided deaf…

Developing and Testing a Self-Regulated Learning Assessment Methodology Combined with Virtual-Patient Simulation in Medical Education

DTIC Science & Technology

2015-04-09

Cleary, Dong, & Artino, 2014). Collectively, various ex post facto studies have shown that experts or high achievers display more adaptive forms...regulated learning and academic achievement: A case study analysis [Special Issue]. Educational Research International. doi: 10.1155/2013/272560...Services University of the Health Sciences 4301 Jones Bridge Road Bethesda, Maryland 20814 AF Medical Research Program Air Force Surgeon General

Prediction of Protection against Asian Enterovirus 71 Outbreak Strains by Cross-neutralizing Capacity of Serum from Dutch Donors, The Netherlands

PubMed Central

Koen, Gerrit; van Eijk, Hetty; Koekkoek, Sylvie M.; de Jong, Menno D.; Wolthers, Katja C.

2016-01-01

Outbreaks of human enterovirus 71 (EV-71) in Asia are related to high illness and death rates among children. To gain insight into the potential threat for the population of Europe, we determined the neutralizing activity in intravenous immunoglobulin (IVIg) batches and individual serum samples from donors in the Netherlands against EV-71 strains isolated in Europe and in Asia. All IVIg batches and 41%, 79%, and 65% of serum samples from children ≤5 years of age, women of childbearing age, and HIV-positive men, respectively, showed high neutralizing activity against a Dutch C1 strain, confirming widespread circulation of EV-71 in the Netherlands. Asian B3–4 and C4 strains were efficiently cross-neutralized, predicting possible protection against extensive circulation and associated outbreaks of those types in Europe. However, C2 and C5 strains that had few mutations in the capsid region consistently escaped neutralization, emphasizing the importance of monitoring antigenic diversity among circulating EV-71 strains. PMID:27533024

21 CFR 201.71 - Magnesium labeling.

Code of Federal Regulations, 2014 CFR

2014-04-01

... disease [bullet] a magnesium-restricted diet”. The warnings in §§ 201.64(c), 201.70(c), 201.71(c), and 201...., a magnesium or potassium-restricted diet. 1 See § 201.66(b)(4) of this chapter for definition of...

21 CFR 201.71 - Magnesium labeling.

Code of Federal Regulations, 2012 CFR

2012-04-01

... disease [bullet] a magnesium-restricted diet”. The warnings in §§ 201.64(c), 201.70(c), 201.71(c), and 201...., a magnesium or potassium-restricted diet. 1 See § 201.66(b)(4) of this chapter for definition of...

21 CFR 201.71 - Magnesium labeling.

Code of Federal Regulations, 2013 CFR

2013-04-01

... disease [bullet] a magnesium-restricted diet”. The warnings in §§ 201.64(c), 201.70(c), 201.71(c), and 201...., a magnesium or potassium-restricted diet. 1 See § 201.66(b)(4) of this chapter for definition of...

Lactic acid bacteria found in fermented fish in Thailand.

PubMed

Tanasupawat, Somboon; Okada, Sanae; Komagata, Kazuo

1998-06-01

Forty-seven strains of homofermentative rod-shaped and 5 heterofermentative sphere-shaped lactic acid bacteria were isolated from 4 kinds of fermented fish (pla-ra, pla-chom, kung-chom, and hoi-dong) in Thailand. These bacteria were separated into four groups by phenotypic and chemotaxonomic characteristics, including fluorometric DNA-DNA hybridization. Five strains (Group I) contained meso-diaminopimelic acid in the cell wall. Four strains were identified as Lactobacillus pentosus, and one strain was L. plantarum. Tested strains of this group produced DL-lactic acid. The rest of the rod-shaped bacteria, 23 strains (Group II) and 19 strains (Group III), lacked meso-diaminopimelic acid in the cell wall and were identified as L. farciminis and Lactobacillus species, respectively. The tested strains of these groups produced L-lactic acid. The amount of cellular fatty acids of C16:0 and C18:1, and the DNA base compositions were significant for differentiating the strains in Groups II and III. Five strains of cocci in chains (Group IV) produced gas from glucose. The tested strains of this group produced d-lactic acid. They were identified as a Leuconostoc species. The distribution of these bacteria in fermented fish in Thailand is discussed.

71. Historic American Buildings Survey John O. Brostrup, Photographer August ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

71. Historic American Buildings Survey John O. Brostrup, Photographer August 12, 1936 1:30 P. M. VIEW OF C.C.C. BOYS EXCAVATING IN UNIT A. - General John Mason House, Analostan Island or Theodore Roosevelt Island, Washington, District of Columbia, DC

Differential imprints of different ENSO flavors in global patterns of seasonal precipitation extremes

NASA Astrophysics Data System (ADS)

Wiedermann, Marc; Siegmund, Jonatan F.; Donges, Jonathan F.; Donner, Reik V.

2017-04-01

The El Nino Southern Oscillation (ENSO) with its positive (El Nino) and negative (La Nina) phases is known to trigger climatic responses in various parts of the Earth, an effect commonly attributed to teleconnectivity. A series of studies has demonstrated that El Nino periods exhibits a relatively broad variety of spatial patterns, which can be classified into two main flavors termed East Pacific (EP, canonical) and Central Pacific (CP, Modoki) El Nino, and that both subtypes can trigger distinct climatic responses like droughts vs. precipitation increases at the regional level. More recently, a similar discrimination of La Nina periods into two different flavors has been reported, and it is reasonable to assume that these different expressions are equally accompanied by differential responses of regional climate variability in particularly affected regions. In this work, we study in great detail the imprints of both types of El Nino and La Nina periods in extremal seasonal precipitation sums during fall (SON), winter (DJF) and spring (MAM) around the peak time of the corresponding ENSO phase. For this purpose, we employ a recently developed objective classification of El Nino and La Nina periods into their two respective flavors based on global teleconnectivity patterns in daily surface air temperature anomalies as captured by the associated climate network representations (Wiedermann et al., 2016). In order to study the statistical relevance of the timing of different El Nino and La Nina types on that of seasonal precipitation extremes around the globe (according to the GPCC data set as a reference), we utilize event coincidence analysis (Donges et al., 2016), a new powerful yet conceptually simple and intuitive statistical tool that allows quantifying the degree of simultaneity of distinct events in pairs of time series. Our results provide a comprehensive overview on ENSO related imprints in regional seasonal precipitation extremes. We demonstrate that key interlinkages between ENSO phases and droughts as well as extremely wet seasons depend crucially on the specific type of El Nino and La Nina event, highlighting the importance of correctly attributing the corresponding flavors when aiming to anticipate the likelihood of precipitation extremes. Straightforward upcoming extensions of the present work will address the imprints of ENSO types and flavors on extremes at different time scales that can be found in other relevant climate variables such as air temperature or more complex drought indices, as well as an assessment of the representation of the empirically found statistical relationships in contemporary climate models operated in hindcast as well as RCP scenario modes. M. Wiedermann, A. Radebach, J.F. Donges, J. Kurths, R.V. Donner: A climate network-based index to discriminate different types of El Nino and La Nina. Geophysical Research Letters, 43, 069119 (2016) J.F. Donges, C.-F. Schleussner, J.F. Siegmund, R.V. Donner: Event coincidence analysis for quantifying statistical interrelationships between event time series - On the role of extreme flood events as possible drivers of epidemics. European Physical Journal - Special Topics, 225(3), 471-487 (2016)

JPRS Report, Science & Technology, China: Energy

DTIC Science & Technology

1988-06-29

capacity. There are currently two types of HTGR reactor designs: the particle-bed core , which uses spherical fuel elements, and the rod type core , in...and trial operating experience with the HTGR reactor. Its main design features are as follows. 1. A particle-bed core , continuous fueling and...Favorable for Development of Small-Scale HTGR (Xu Jiming; HE DONGLI GONGCHENG, Feb 88) 47 ERRATUM: In JPRS-CEN-88-003 of 25 April 1988 in article

Food and Agricultural Imports from China

DTIC Science & Technology

2007-07-17

2006. Among the product categories that at least doubled in volume during the period were live animals, wine/ beer , fruit/vegetable juices, wheat...product had complied with safe manufacturing practices (e.g., HACCP for low acid canned foods or seafoods).22 Refusals of Imports from China Of the 720...Phytosanitary Measures,” Choices, 1st quarter 2007. 30 Calvin. 31 Dong. 32 FDA information on HACCP is at [http://www.cfsan.fda.gov/~lrd/haccp.html]. 33

A Clinician-Centered Evaluation of the Usability of AHLTA and Automated Clinical Practice Guidelines at TAMC

DTIC Science & Technology

2009-10-01

gathering has been rescheduled to November 14, 2009, in San Francisco, at the AMIA Conference location. August 14, 2009, SOADEX provided a draft system...Bethesda. MD, USA d Core Unit for Medical Statistics and Informatics, Medical University of Vienna, Austria • Department of Nursing . lnha University...scheduled. Insook Cho, PhD, RN Associate Professor, Maternity Nursing & Nursing Informat- ics, lnha University, Younghyun-dong, Nam-gu, Jncheon, South

Recommendations for recognizing video events by concept vocabularies

DTIC Science & Technology

2014-06-01

authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright anno - tation thereon. Disclaimer: The views and...detection and recognition for semantic annotation of video, Multimedia Tools Appl. 51 (1) (2011) 279–302. [6] A. Berg, J . Deng, S. Satheesh, H. Su, F.-F...Li, Imagenet Large Scale Visual Recognition Challenge 2011. <http://www.image-net.org/challenges/LSVRC/ 2011/>. [7] J . Deng, W. Dong, R. Socher, L

Armed Forces and National Development in Korea

DTIC Science & Technology

1988-12-01

2698 Naval Postgraduate School Monterey, CA 93943 7. Ha, Tae Hwan SMC 2588 Naval Postgraduate School Monterey, CA 93943 8. Kim , Dong Hui SMC 2610 Naval...an agreement between the Provisional Government leader Kim Ku and Chiang Kai-Shek. Many of these Korean officers, mostly the Japanese-trained, played...when the military took over the corrupt and inefficient government [Ref. 6:p. 36]. Kim said that the military assumption of power in 1961 was

Increase of Farmers' Knowledge through Farmer Seed Production Schools in Vietnam as Assessed on the Basis of Ex-Ante and Ex-Post Tests

ERIC Educational Resources Information Center

Tin, Huynh Q.; Struik, Paul C.; Price, Lisa L.; Tuyen, Nguyen P.; Hoan, Nguyen P.; Bos, Heleen

2010-01-01

The study was designed to assess changes in farmers' knowledge of farmer seed production through schools (FSPSs) in Vietnam. A set of 25 questions covering five technical areas of the seed production process was used for pre and post knowledge testing at 12 FSPSs in the provinces Binh Dinh, Nam Dinh, Nghe An and Dong Thap. The main findings show…

"Embodiment as procedures: Physical cleansing changes goal priming effects": Correction to Dong and Lee (2017).

PubMed

2017-06-01

Reports an error in "Embodiment as procedures: Physical cleansing changes goal priming effects" by Ping Dong and Spike W. S. Lee ( Journal of Experimental Psychology: General , 2017[Apr], Vol 146[4], 592-605). In the article, the following F-value and p-values in the Results section of Experiment 3 were set incorrectly: The p -value p =.925 should be p =.922. The F - and p -values F (1, 201)=.011, p =.916 should be F (1, 201) .014, p = .906. (The following abstract of the original article appeared in record 2017-14922-010.) Physical cleansing reduces the influence of numerous psychological experiences, such as guilt from immoral behavior, dissonance from free choice, and good/bad luck from winning/losing. How do these domain-general effects occur? We propose an integrative account of cleansing as an embodied procedure of psychological separation. By separating physical traces from a physical target object (e.g., detaching dirt from hands), cleansing serves as the embodied grounding for the separation of psychological traces from a psychological target object (e.g., dissociating prior experience from the present self). This account predicts that cleansing reduces the accessibility of psychological traces and their consequences for judgments and behaviors. Testing these in the context of goal priming, we find that wiping one's hands (vs. not) decreases the mental accessibility (Experiment 1), behavioral expression (Experiment 2), and judged importance (Experiments 3-4) of previously primed goals (e.g., achievement, saving, fitness). But if a goal is primed after cleansing, its importance gets amplified instead (Experiment 3). Based on the logic of moderation-of-process, an alternative manipulation that psychologically separates a primed goal from the present self produces the same effects, but critically, the effects vanish once people wipe their hands clean (Experiment 4), consistent with the notion that cleansing functions as an embodied procedure of psychological separation. These findings have implications for the flexibility of goal pursuit. More broadly, our procedural perspective generates novel predictions about the scope and mechanisms of cleansing effects and may help integrate embodied and related phenomena. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

TSAR (Theater Simulation of Airbase Resources) Database Dictionary F-4G.

DTIC Science & Technology

1987-06-05

REC/TRANS RT-1159 388 71ZDO CNTL UNIT C- 10062 /A 391 71320 CNTL ARN-127 393 723A0 REC/TRANS RT-689 394 723B0 INDIC, HEIGHT TOTAL NUMBER OF PART REPAIR...PROCEDURES - 2 LOU " 4"p" IW qg t~l pb i.go stanc E lm FIGURE 98 111-260 S.. RESOURCE REQUIREMENTS 111.1.5.25 LIU’S #45 - #49 - *LRU PART TIME...RT-1159 AG 386 71ZBO ADAPTER MX9577 AG 387 71ZCO MOUNT (REC/TRANS) AG 388 71ZDO CNTL UNIT C- 10062 /A AG 389 71ZEO MOUNT (DIG TO ANALOG CONVERTER) AG

Effects of Neodymium and Calcium on the Thermal Stability of AZ71 Magnesium Alloys

NASA Astrophysics Data System (ADS)

Yue, Cheng-Feng; Huang, Shi-Jei; Chen, Jhewn-Kuang; Li, Hsien-Tsung; Chan, Kam-Shau

2018-03-01

The effects of an addition of 0-2 wt% Nd on thermal stability of 0-3 wt% Ca-containing modified AZ71 magnesium alloys was investigated. The ignition temperature was found to increase from that of AZ71, 574, to 825 °C with the addition of 0.5 wt% Ca and 1 wt% Nd. The ignition temperature was further increased to 1114 °C when 3 wt% Ca was added. The Ca- and Nd-added AZ71 was isothermally maintained at a temperature of 500 °C in air for 12 h. The MgO-CaO-Nd2O3 formed on the surface to improve the thermal stability of the AZ71-xCa-yNd alloys. While both the tensile strength and ductility decreased with the Ca concentration in the alloy, an addition of 1 wt% Nd was found able to alleviate the degradation effects of Ca on the tensile strength and ductility at 170 °C. Both solid solution formation and precipitation strengthening contributed to the increase in toughness. AZ71 containing 0.5-2 wt% Ca and 1 wt% Nd provides the optimum combination of ignition resistance and mechanical properties.

Development of new polyphenols applied to spin-on carbon hardmask with characteristics of high-heat resistance and good planarization

NASA Astrophysics Data System (ADS)

Horiuchi, Junya; Takigawa, Tomoaki; Shimizu, Yoko; Makinoshima, Takashi; Sato, Takashi; Echigo, Masatoshi

2018-03-01

We had developed various Polyphenols and Xanthene derivatives such as NF7177C and NF0197 which showed high heat resistance. In this paper, we reported on new Xanthene derivatives and Polyphenols with characteristics of not only high heat resistance but also low Tg which contributes to good planarization characteristic of Spin-On Carbon Hardmask [1]. One approach to decrease Tg is introducing alkyl group into the Xanthene derivatives. We synthesized the new Xanthene derivatives by the reaction of dihydroxynaphthalene with the aldehydes containing alkyl group. These Xanthene derivatives containing alkyl group showed lower Tg than those not containing alkyl group by about 20 30°C. Another approach to decrease Tg is increasing flexibility of molecular structure. We synthesized the new Polyphenols (NF71C7, NF71D7, NF91A7, and NF71A7) by the reaction of flexible or asymmetric phenols such as 4,4'- dihydroxydiphenyl ether or 4-phenylphenol. The Tg of these Polyphenols was 70 80°C and these values were much lower than the existing polyphenol (NF7177C) synthesized from 4,4-biphenol by about 50°C. We evaluated heat resistance of these Polyphenols with additive, and both NF71C7 and NF71D7 showed comparable heat resistance to NF0197 and NF7177C.

Rhodonellum psychrophilum gen. nov., sp. nov., a novel psychrophilic and alkaliphilic bacterium of the phylum Bacteroidetes isolated from Greenland.

PubMed

Schmidt, Mariane; Priemé, Anders; Stougaard, Peter

2006-12-01

A novel alkaliphilic and psychrophilic bacterium was isolated from the cold and alkaline ikaite tufa columns of the Ikka Fjord in south-west Greenland. According to 16S rRNA gene sequence analysis, strain GCM71(T) belonged to the family 'Flexibacteraceae' in the phylum Bacteroidetes. Strain GCM71(T), together with five related isolates from ikaite columns, formed a separate cluster with 86-93 % gene sequence similarity to their closest relative, Belliella baltica. The G+C content of the DNA from strain GCM71(T) was 43.1 mol%, whereas that of B. baltica was reported to be 35 mol%. DNA-DNA hybridization between strain GCM71(T) and B. baltica was 9.5 %. The strain was red pigmented, Gram-negative, strictly aerobic with non-motile, rod-shaped cells. The optimal growth conditions for strain GCM71(T) were pH 9.2-10.0, 5 degrees C and 0.6 % NaCl. The fatty acid profile of the novel strain was dominated by branched and unsaturated fatty acids (90-97 %), with a high abundance of iso-C(17 : 1)omega9c (17.5 %), iso-C(17 : 0) 3-OH (17.5 %) and summed feature 3, comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c (12.6 %). Phylogenetic, chemotaxonomic and physiological characteristics showed that the novel strain could not be affiliated to any known genus. A new genus, Rhodonellum gen. nov., is proposed to accommodate the novel strain. Strain GCM71(T) (=DSM 17998(T)=LMG 23454(T)) is proposed as the type strain of the type species, Rhodonellum psychrophilum sp. nov.

A Proposed Activity for a Meaningful Learning about the Moon Phases. (Breton Title: Uma Proposta de Atividade Para a Aprendizagem Significativa sobre as Fases da Lua.) Una Actividad Propuesta Para EL Aprendizaje Significativo Acerca de Las Fases de la Luna

NASA Astrophysics Data System (ADS)

Martins, Bruno Andrade; Langhi, Rodolfo

2012-12-01

This paper presents one of the concepts of Astronomy and its consequent failure in teaching this topic in high school, even when the official documents point out the necessity of Astronomy teaching at this school level. Among the spontaneous conceptions in Astronomy that high school students carry with them, even after the end of the school, we emphasized in this research the Moon phases. The development of different strategies in relation to traditional methods, aimed to teaching-learning process on this topic was considered in this study. These strategies were devised based on the reference frame of the Meaningful Learning, as elaborated by Ausubel. The proposals presented here include the active participation of students in experimental activities and other didactic activities, for their continuous evaluation during the process. These activities finished with a Comics elaboration about the Moon phases. Therefore, the objective of this paper is to present a proposal for differentiated teaching activity about Moon phases supported by the theoretical principles of Meaningful Learning at Physics classes. Este texto foca um dos conteúdos de Astronomia e a consequente falha no ensino deste tema no ensino médio, apesar de os documentos oficiais apresentarem a necessidade de se trabalhar a Astronomia neste nível de ensino. Dentre as concepções alternativas em Astronomia que os alunos do ensino médio carregam consigo, mesmo após o término dos estudos, destacamos, nesta pesquisa, o fenômeno das fases da Lua. O desenvolvimento de estratégias diferenciadas em relação ao ensino tradicional, visando o processo de ensino-aprendizagem sobre este tema, foi contemplado neste trabalho como um dos resultados obtidos sob a luz dos referenciais da aprendizagem significativa, fundamentados em Ausubel. Segundo a proposta aqui apresentada, a participação ativa dos alunos na execução de uma atividade experimental e outras atividades didáticas, que visam sua contínua avaliação durante o processo, culmina com a elaboração de uma história em quadrinhos envolvendo as fases da Lua. Portanto, o objetivo deste trabalho é apresentar uma proposta de atividade didática diferenciada sustentada pelos princípios teóricos da aprendizagem significativa aplicada ao ensino das fases da Lua durante as aulas de Física no ensino médio. En este trabajo se presenta uno de los contenidos de la Astronomía y el evidente fracaso en la enseñanza del tema en la escuela secundaria, a pesar del hecho que los documentos oficiales apuntan para la necesidad de trabajar contenidos de Astronomía en este nivel. Entre los conceptos alternativos en Astronomía que los alumnos secundarios llevan consigo, aún después de terminados los estudios, destacamos aquí el fenómeno de las fases de la Luna. El desarrollo de diferentes estrategias en relación con los métodos tradicionales, dirigidas al proceso de enseñanza-aprendizaje en este tema fue considerado en este trabajo como uno de los resultados obtenidos a la luz de los referenciales del aprendizaje significativo, tal como fueron fundamentados por Ausubel. Según la propuesta que aquí se presenta, la participación activa de los estudiantes en la ejecución de una actividad experimental y otras actividades educativas destinadas a la evaluación continua durante el proceso culminó en la elaboración de una historieta respecto de las fases de la Luna. Por lo tanto, el objetivo de este trabajo es presentar una propuesta de actividad de enseñanza diferenciada con el apoyo de los principios teóricos del aprendizaje significativo aplicado a la enseñanza de las fases de la luna durante las clases de física del ciclo secundario.

Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States

PubMed Central

Yang, Zhongzheng; Zhu, Jing; Ying, Tianlei; Jiang, Xianwang; Zhang, Xu; Wu, Houming; Liu, Maili; Tan, Xiangshi; Cao, Chunyang; Huang, Zhong-Xian

2011-01-01

To convert cyt c into a peroxidase-like metalloenzyme, the P71H mutant was designed to introduce a distal histidine. Unexpectedly, its peroxidase activity was found even lower than that of the native, and that the axial ligation of heme iron was changed to His71/His18 in the oxidized state, while to Met80/His18 in the reduced state, characterized by UV-visible, circular dichroism, and resonance Raman spectroscopy. To further probe the functional importance of Pro71 in oxidation state dependent conformational changes occurred in cyt c, the solution structures of P71H mutant in both oxidation states were determined. The structures indicate that the half molecule of cyt c (aa 50–102) presents a kind of “zigzag riveting ruler” structure, residues at certain positions of this region such as Pro71, Lys73 can move a big distance by altering the tertiary structure while maintaining the secondary structures. This finding provides a molecular insight into conformational toggling in different oxidation states of cyt c that is principle significance to its biological functions in electron transfer and apoptosis. Structural analysis also reveals that Pro71 functions as a key hydrophobic patch in the folding of the polypeptide of the region (aa 50–102), to prevent heme pocket from the solvent. PMID:22087268

Active Control Synthesis for Flexible Vehicles. Volume II. KONPACT program Listing

DTIC Science & Technology

1976-01-01

68 Subroutine TPR Program Listing 69 Subroutine HPR Program Listing 70 Subroutine IDPR Program Listing 71 Subroutine MPRS Program Listing 72...to o n c 1 9 - c ■ ■a 1 1 1 1 E E E CM In 01 i 1 i ^ c i 3 1 2 71 ■ E a ■ E 0 ■ i a. 2 g B c i...CO n CO CO ?o CO CO CO CO CO 1 W 71 OT tn t/; W5 7) 7J 73 n 7) CO 75 75 7) 75 CO 75 75 75 75 75 75 T: 75 75 to 20 MM mi I

10 CFR 71.103 - Quality assurance organization.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 2 2011-01-01 2011-01-01 false Quality assurance organization. 71.103 Section 71.103... Quality Assurance § 71.103 Quality assurance organization. (a) The licensee, 2 certificate holder, and... that are important to safety have been correctly performed. (c) The persons and organizations...

10 CFR 71.103 - Quality assurance organization.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Quality assurance organization. 71.103 Section 71.103... Quality Assurance § 71.103 Quality assurance organization. (a) The licensee, 2 certificate holder, and... that are important to safety have been correctly performed. (c) The persons and organizations...

10 CFR 71.105 - Quality assurance program.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Quality assurance program. 71.105 Section 71.105 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.105 Quality assurance program. (a) The licensee, certificate holder, and applicant for a CoC...

10 CFR 71.135 - Quality assurance records.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Quality assurance records. 71.135 Section 71.135 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.135 Quality assurance records. The licensee, certificate holder, and applicant for a CoC...

10 CFR 71.123 - Test control.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Test control. 71.123 Section 71.123 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.123 Test control. The licensee, certificate holder, and applicant for a CoC shall establish a test...

10 CFR 71.123 - Test control.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 2 2013-01-01 2013-01-01 false Test control. 71.123 Section 71.123 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.123 Test control. The licensee, certificate holder, and applicant for a CoC shall establish a test...

10 CFR 71.123 - Test control.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 2 2014-01-01 2014-01-01 false Test control. 71.123 Section 71.123 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.123 Test control. The licensee, certificate holder, and applicant for a CoC shall establish a test...

An Analysis of Completion Times at Various Army Training Centers of Programmed Instruction Materials for MOS 71 B 10, MOS 71 H 20, & MOS 71 B 20/30.

DTIC Science & Technology

1972-06-01

I I I I oil N 4 N N~ N N0 Cot m CA r N -( qr% W % P- a %.P- ON -Y ON % enm w% -%0 DC 110 oV to 0% 0 0D 0 *n 0n e 00 O N Ln m~ CV) co ONN 11: v- 0...CC’D a) =) c’.C’ C) CC) CD C.)’ L t- I’D CM " C’.JCO.J U/ I U) I r- C40 U)zj )Dm t D C Dc ma m’ CD C) CD 10 co a II I ct C’ ’ ’ ’ 𔃾 (4 (. ’ ’ LUV ...been approv S i fOr Public release and slo; Its distribution is unlimited. 9

Role of TGF-B1-Mediated Down Regulation of NF-kB/Rel Activity During Growth Arrest of Breast Cancer Cells

DTIC Science & Technology

2000-05-01

PRINCIPAL INVESTIGATOR: Dong Kim, M.S.E. Dr. Sonenshein CONTRACTING ORGANIZATION: Boston University Boston, Massachusetts 02118 REPORT DATE: May 2000...TYPE OF REPORT : Annual Summary PREPARED FOR: U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 DISTRIBUTION STATEMENT...Approved for public release; distribution unlimited The views, opinions and/or findings contained in this report are those of the author(s) and should

China and Proliferation of Weapons of Mass Destruction and Missiles: Policy Issues

DTIC Science & Technology

2006-12-11

Ghauri (Hatf-5) medium-range ballistic missile (MRBM), which is based on the North Korean No Dong missile. U.S. intelligence was said to suspect that...confirming these stories, the DCI reported in August 2000 that, besides North Korean help, PRC entities provided “increased assistance” to Pakistan’s...ranges, respectively, of about 800 mi. and 1,250 mi.). Over 100 PRC and North Korean experts worked there, reported the Washington Times (November 23

EGFR-Dependent Regulation of Matrix-Independent Epithelial Cell Survival. Addendum

DTIC Science & Technology

2007-04-01

of the original proposal. The results obtained have identified key players that coordinate keratinocyte survival dependent on soluble growth factors...2004;6:203–8. 4. Duffey DC, Chen Z, Dong G, et al. Expression of a dominant-negative mutant inhibitor- nBa of nuclear fac- tor-nB in human head and neck...Attempts to treat such tumors with EGFR antagonists have met with remarkable initial successes , particularly when EGFR antagonists were used in

Enhancing Communication in Noisy Environments

DTIC Science & Technology

2009-10-01

derived from the ITD and ILD cues, which are binaural . ITD depends on the azimuthal position of the source. Similarly, ILD refers to the fact...4.4 dB No Perceptual Binaural Speech Enhancement [42] 4.5 dB Yes Fuzzy Cocktail Party Processor [25] 7.5 dB Yes Binaural segregation [43] 8.9 dB No...modulation. IEEE Transactions on Neural Networks. 15 (2004): 1135-50. [42] Dong R. Perceptual Binaural Speech Enhancement in Noisy Environments. M.A.Sc

URREF Reliability Versus Credibility in Information Fusion

DTIC Science & Technology

2013-07-01

Fusion, Vol. 3, No. 2, December, 2008. [31] E. Blasch, J. Dezert, and P. Valin , “DSMT Applied to Seismic and Acoustic Sensor Fusion,” Proc. IEEE Nat...44] E. Blasch, P. Valin , E. Bossé, “Measures of Effectiveness for High- Level Fusion,” Int. Conference on Information Fusion, 2010. [45] X. Mei, H...and P. Valin , “Information Fusion Measures of Effectiveness (MOE) for Decision Support,” Proc. SPIE 8050, 2011. [49] Y. Zheng, W. Dong, and E

Evaluating physical and perceptual responses to exergames in Chinese children.

PubMed

Lau, Patrick W C; Liang, Yan; Lau, Erica Y; Choi, Choung-Rak; Kim, Chang-Gyun; Shin, Myung-Soo

2015-04-13

The primary objective of this study was to examine whether exergames could help children reach the recommendations for PA and cardiorespiratory fitness regarding exercise intensity. Differences in perceived physical exertion, EE, VO2, and HR between normal weight (NW) and overweight (OW) children participating in exergames were also examined. Twenty-one children (age: 10.45 ± 0.88) were assessed for EE, VO2 and HR during rest, in a maximal treadmill test, and while playing different exergames. Ratings of perceived exertion (RPE) (category range: 0 to 10) were also measured during exergaming. Three types of exergames were examined: running, table tennis, and dancing. These games were either performed on a Chinese game console, I-Dong, or another well-developed Western game console (Sony PlayStation 3 or Nintendo Wii). Exergaming resulted in EE (kcal/min) from 2.05-5.14, VO2 (mL/kg/min) from 9.98-25.54, and HR (beats per minute) from 98.05-149.66. Children reported RPE ranging from 1.29 to 5.29. The Chinese exergame, I-Dong Running, was the only game in which children reached a moderate intensity and met the recommended minimum VO2reserve (50%) for cardiorespiratory fitness. Exergames could provide alternative opportunities to enhance children's physical activity. They could be used as light-to-moderate PA, and with exergames, children can even reach the recommended intensity for developing and maintaining cardiorespiratory fitness.

Broadband enhanced transmission of acoustic waves through serrated metal gratings

NASA Astrophysics Data System (ADS)

Qi, Dong-Xiang; Fan, Ren-Hao; Deng, Yu-Qiang; Peng, Ru-Wen; Wang, Mu; Jiangnan University Collaboration

In this talk, we present our studies on broadband properties of acoustic waves through metal gratings. We have demonstrated that serrated metal gratings, which introduce gradient coatings, can give rise to broadband transmission enhancement of acoustic waves. Here, we have experimentally and theoretically studied the acoustic transmission properties of metal gratings with or without serrated boundaries. The average transmission is obviously enhanced for serrated metal gratings within a wide frequency range, while the Fabry-Perot resonance is significantly suppressed. An effective medium hypothesis with varying acoustic impedance is proposed to analyze the mechanism, which was verified through comparison with finite-element simulation. The serrated boundary supplies gradient mass distribution and gradient normal acoustic impedance, which could efficiently reduce the boundary reflection. Further, by increasing the region of the serrated boundary, we present a broadband high-transmission grating for wide range of incident angle. Our results may have potential applications to broadband acoustic imaging, acoustic sensing and new acoustic devices. References: [1] Dong-Xiang Qi, Yu-Qiang Deng, Di-Hu Xu, Ren-Hao Fan, Ru-Wen Peng, Ze-Guo Chen, Ming-Hui Lu, X. R. Huang and Mu Wang, Appl. Phys. Lett. 106, 011906 (2015); [2] Dong-Xiang Qi, Ren-Hao Fan, Ru-Wen Peng, Xian-Rong Huang, Ming-Hui Lu, Xu Ni, Qing Hu, and Mu Wang, Applied Physics Letters 101, 061912 (2012).

The baseline characteristics and interim analyses of the high-risk sentinel cohort of the Vietnam Initiative on Zoonotic InfectiONS (VIZIONS)

PubMed Central

Carrique-Mas, Juan J.; Tue, Ngo T.; Bryant, Juliet E.; Saylors, Karen; Cuong, Nguyen V.; Hoa, Ngo T.; An, Nguyen N.; Hien, Vo B.; Lao, Pham V.; Tu, Nguyen C.; Chuyen, Nguyen K.; Chuc, Nguyen T.K.; Tan, Dinh V.; Duong, Hoang Van V.; Toan, Tran K.; Chi, Nguyen T.Y.; Campbell, James; Rabaa, Maia A.; Nadjm, Behzad; Woolhouse, Mark; Wertheim, Heiman; Thwaites, Guy; Baker, Stephen

2015-01-01

The Vietnam Initiative for Zoonotic Infections (VIZIONS) includes community-based ‘high-risk sentinel cohort’ (HRSC) studies investigating individuals at risk of zoonotic infection due to occupational or residential exposure to animals. A total of 852 HRSC members were recruited between March 2013 and August 2014 from three provinces (Ha Noi, Dak Lak, and Dong Thap). The most numerous group (72.8%) corresponded to individuals living on farms, followed by slaughterers (16.3%) and animal health workers (8.5%). Nasal/pharyngeal and rectal swabs were collected from HRSC members at recruitment and after notifying illness. Exposure to exotic animals (including wild pigs, porcupine, monkey, civet, bamboo rat and bat) was highest for the Dak Lak cohort (53.7%), followed by Ha Noi (13.7%) and Dong Thap (4.0%). A total of 26.8% of individuals reported consumption of raw blood over the previous year; 33.6% slaughterers reported no use of protective equipment at work. Over 686 person-years of observation, 213 episodes of suspect infectious disease were notified, equivalent of 0.35 reports per person-year. Responsive samples were collected from animals in the farm cohort. There was noticeable time and space clustering of disease episodes suggesting that the VIZIONS set up is also suitable for the formal epidemiological investigation of disease outbreaks. PMID:26659094

Osmoregulation and antioxidant production in maize under combined cadmium and arsenic stress.

PubMed

Anjum, Shakeel Ahmad; Tanveer, Mohsin; Hussain, Saddam; Shahzad, Babar; Ashraf, Umair; Fahad, Shah; Hassan, Waseem; Jan, Saad; Khan, Imran; Saleem, Muhammad Farrukh; Bajwa, Ali Ahsan; Wang, Longchang; Mahmood, Aqib; Samad, Rana Abdul; Tung, Shahbaz Atta

2016-06-01

An investigation was carried out to examine the combined and individual effects of cadmium (Cd) and arsenic (As) stress on osmolyte accumulation, antioxidant activities, and reactive oxygen species (ROS) production at different growth stages (45, 60, 75, 90 days after sowing (DAS)) of two maize cultivars viz., Dong Dan 80 and Run Nong 35. The Cd (100 μM) and As (200 μM) were applied separately as well as in combination (Cd + As) at 30 DAS. Results revealed pronounced variations in the behavior of antioxidants, osmolytes, and ROS in both maize cultivars under the influence of Cd and As stress. Activities of enzymatic (SOD, POD, CAT and APX, GPX, GR) and non-enzymatic (GSH and AsA) antioxidants, generation of ROS, and accumulation of osmolytes were enhanced with the passage of time; therefore, the maximum values for these attributes were observed at 90 DAS for both cultivars. Exposure of plants to Cd or As stress considerably enhanced the antioxidant activities, ROS, and osmolyte accumulation compared with control, while combined application of Cd + As was more devastating in reducing plant biomass of both maize cultivars. Among cultivars, Dong Dan 80 was better able to negate the heavy metal-induced oxidative damage, which was associated with higher antioxidant activities, greater osmolytes accumulation, and lower ROS production in this cultivar.

Temperature dependence of the electrical resistivity of LaxLu1-xAs

NASA Astrophysics Data System (ADS)

Rahimi, S.; Krivoy, E. M.; Lee, J.; Michael, M. E.; Bank, S. R.; Akinwande, D.

2013-08-01

We investigate the temperature-dependent resistivity of single-crystalline films of LaxLu1-xAs over the 5-300 K range. The resistivity was separated into lattice, carrier and impurity scattering regions. The effect of impurity scattering is significant below 20 K, while carrier scattering dominates at 20-80 K and lattice scattering dominates above 80 K. All scattering regions show strong dependence on the La content of the films. While the resistivity of 600 nm LuAs films agree well with the reported bulk resistivity values, 3 nm films possessed significantly higher resistivity, suggesting that interfacial roughness significantly impacts the scattering of carriers at the nanoscale limit.

Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012.

PubMed

Donato, Celeste; Hoi, Le Thi; Hoa, Nguyen Thi; Hoa, Tran Mai; Van Duyet, Le; Dieu Ngan, Ta Thi; Van Kinh, Nguyen; Vu Trung, Nguyen; Vijaykrishna, Dhanasekaran

2016-08-01

Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities. A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected. Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases. Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

3D-quantitative structure-activity relationship study for the design of novel enterovirus A71 3C protease inhibitors.

PubMed

Nie, Quandeng; Xu, Xiaoyi; Zhang, Qi; Ma, Yuying; Yin, Zheng; Shang, Luqing

2018-06-07

A three-dimensional quantitative structure-activity relationships model of enterovirus A71 3C protease inhibitors was constructed in this study. The protein-ligand interaction fingerprint was analyzed to generate a pharmacophore model. A predictive and reliable three-dimensional quantitative structure-activity relationships model was built based on the Flexible Alignment of AutoGPA. Moreover, three novel compounds (I-III) were designed and evaluated for their biochemical activity against 3C protease and anti-enterovirus A71 activity in vitro. III exhibited excellent inhibitory activity (IC 50 =0.031 ± 0.005 μM, EC 50 =0.036 ± 0.007 μM). Thus, this study provides a useful quantitative structure-activity relationships model to develop potent inhibitors for enterovirus A71 3C protease. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Tyndall AFB, Florida. Revised Uniform Summary of Surface Weather Observations. Parts A-F.

DTIC Science & Technology

1987-07-24

OF RECORD: 71 -86 MONTHz DEC RAIN FRZIHN SNOW t OS SMOKE DUST % 08S HOURS I TSTMS LIOR RAIN cIOR MAIL WITH FOG C/OR BLOWING L/OR W/0BST TOTAL ILSTI I...1 133S1 5.71 23.60 .00 OCT 71 .b i 8.21 1.3 1 2.51 1.41 2.81 Z.51 ?.4 1 2.3 1 .7 1 -1 1 1 I 16.0 1 13871 3.12 16.11 .0c NOV 66.2 1 6.31 1.1 1 6.31...ae 2.C9 .76 1.66 Y.7- 1.6! *3.79 4.33 1.61 2.10 $4.33 b5 I .67 1.S!5 Z. 71 2.51; TRACE 4.4b 2.--- 2.bS 2.62 3.341 .95 2.14 4.46 66 1 1.32 Z.42 ’.’L

[Breaking paradigms. Intestinal microbiota transplantation: Preliminar report].

PubMed

Zamudio-Tiburcio, Álvaro; Bermúdez-Ruiz, Héctor; Lezama-Guzmán, Hugo Ricardo; Guevara-Ortigoza, María Del Pilar; Islas-Solares, Elena; Sosa-López, Francisco Antonio

2017-12-01

In the fourth century, during the Chinese Dong Jin dynasty, the doctor Ge Hong described good results after the oral administration of a suspension prepared from human faeces in patients with severe diarrhoea or food poisoning. Faecal microbiota transplantation has been used for five years in order to treat different diseases in addition to the severe diarrhoea caused by Clostridium difficile 1 . This paper aims to confirm that intestinal microbiota transplantation succeeds in reducing the negative impact of diseases such as severe diarrhoea, irritable bowel syndrome, anxiety, allergies, metabolic syndrome and others and that it is not only indicated for severe diarrhoea caused by C. difficile. This preliminary study included six patients who underwent faecal microbiota transplantation, aged 83, 76, 66, 37 and 36 years (four men and two women). An improvement in symptoms of 70% was observed. The methodology and criteria to be followed with donors are described and the results are listed in three tables. The methodology followed for the microbiota transplant is the same as that reported by other researchers for the treatment of C. difficile diarrhoea and other diseases. The discussion addresses the issues raised in other parts of the world in handling different pathologic entities, as well as genetic advances. The conclusions show encouraging results. Copyright © 2017 Academia Mexicana de Cirugía A.C. Publicado por Masson Doyma México S.A. All rights reserved.

Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice.

PubMed

Madhaiyan, Munusamy; Poonguzhali, Selvaraj; Kwon, Soon-Wo; Sa, Tong-Min

2009-01-01

A pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, CBMB27T, isolated from leaf tissues of rice (Oryza sativa L. 'Dong-Jin'), was analysed using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium oryzae, Methylobacterium fujisawaense and Methylobacterium mesophilicum; strain CBMB27T showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strains of these three species. DNA-DNA hybridization experiments revealed low levels (<38 %) of DNA-DNA relatedness between strain CBMB27T and its closest relatives. The sequence of the 1-aminocyclopropane-1-carboxylate deaminase gene (acdS) in strain CBMB27T differed from those of close relatives. The major fatty acid of the isolate was C(18 : 1)omega7c and the G+C content of the genomic DNA was 66.8 mol%. Based on the results of 16S rRNA gene sequence analysis, DNA-DNA hybridization, and physiological and biochemical characterization, which enabled the isolate to be differentiated from all recognized species of the genus Methylobacterium, it was concluded that strain CBMB27T represents a novel species in the genus Methylobacterium for which the name Methylobacterium phyllosphaerae sp. nov. is proposed (type strain CBMB27T =LMG 24361T =KACC 11716T =DSM 19779T).

30 CFR 71.202 - Certified person; sampling.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Certified person; sampling. 71.202 Section 71... Sampling Procedures § 71.202 Certified person; sampling. (a) The respirable dust sampling required by this... on sampling of respirable coal mine dust. (c) A person may be temporarily certified by MSHA to take...

30 CFR 71.202 - Certified person; sampling.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Certified person; sampling. 71.202 Section 71... Sampling Procedures § 71.202 Certified person; sampling. (a) The respirable dust sampling required by this... on sampling of respirable coal mine dust. (c) A person may be temporarily certified by MSHA to take...

75 FR 66794 - Notice of Affirmative Determination Regarding Application for Reconsideration: TA-W-71,572...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-29

...., Steubenville, OH By applications dated May 15 and May 21, 2010, United Steel, Paper and Forestry, Rubber... back plate coils (TA-W-71,572A), hot rolled coils (TA- W-71,572B), and cold rolled coils (TA-W-71,572C...

10 CFR 71.107 - Package design control.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 2 2014-01-01 2014-01-01 false Package design control. 71.107 Section 71.107 Energy... Assurance § 71.107 Package design control. (a) The licensee, certificate holder, and applicant for a CoC... identification and control of design interfaces and for coordination among participating design organizations...

10 CFR 71.107 - Package design control.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 2 2012-01-01 2012-01-01 false Package design control. 71.107 Section 71.107 Energy... Assurance § 71.107 Package design control. (a) The licensee, certificate holder, and applicant for a CoC... identification and control of design interfaces and for coordination among participating design organizations...

10 CFR 71.107 - Package design control.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 2 2013-01-01 2013-01-01 false Package design control. 71.107 Section 71.107 Energy... Assurance § 71.107 Package design control. (a) The licensee, certificate holder, and applicant for a CoC... identification and control of design interfaces and for coordination among participating design organizations...

10 CFR 71.107 - Package design control.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 2 2011-01-01 2011-01-01 false Package design control. 71.107 Section 71.107 Energy... Assurance § 71.107 Package design control. (a) The licensee, certificate holder, and applicant for a CoC... identification and control of design interfaces and for coordination among participating design organizations...

10 CFR 71.107 - Package design control.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Package design control. 71.107 Section 71.107 Energy... Assurance § 71.107 Package design control. (a) The licensee, certificate holder, and applicant for a CoC... identification and control of design interfaces and for coordination among participating design organizations...

32 CFR 842.71 - Delegations of authority.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false Delegations of authority. 842.71 Section 842.71 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE CLAIMS AND LITIGATION ADMINISTRATIVE CLAIMS International Agreement Claims (10 U.S.C. 2734a and 2734b) § 842.71 Delegations of...

48 CFR 2452.232-71 - Voucher submission (cost-reimbursement).

Code of Federal Regulations, 2010 CFR

2010-10-01

...-reimbursement). 2452.232-71 Section 2452.232-71 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND... Clauses 2452.232-71 Voucher submission (cost-reimbursement). As prescribed in 2432.908(c)(2), insert a clause substantially the same as the following in all cost-reimbursement solicitations and contracts...

The Effect of C-Terminal Helix on the Stability of FF Domain Studied by Molecular Dynamics Simulation

PubMed Central

Zhao, Liling; Cao, Zanxia; Wang, Jihua

2012-01-01

To investigate the effect of C-terminal helix on the stability of the FF domain, we studied the native domain FF3-71 from human HYPA/FBP11 and the truncated version FF3-60 with C-terminal helix being deleted by molecular dynamics simulations with GROMACS package and GROMOS 43A1 force field. The results indicated that the structures of truncated version FF3-60 were evident different from those of native partner FF3-71. Compared with FF3-71, the FF3-60 lost some native contacts and exhibited some similar structural characters to those of intermediate state. The C-terminal helix played a major role in stabilizing the FF3-71 domain. To a certain degree, the FF domain had a tendency to form an intermediate state without the C-terminal helix. In our knowledge, this was the first study to examine the role of C-terminal helix of FF domain in detail by molecular dynamics simulations, which was useful to understand the three-state folding mechanism of the small FF domain. PMID:22408419

Flaring Activity from S5 0836+71 (4C71.07): What Can We Learn with Limited Multiwavelength Coverage?

NASA Technical Reports Server (NTRS)

Thompson, D. J.; Donato, D.; Akyuz, A.; Fuhrmann, L.; Sokolovsky, K.; Kurtanidze, O.

2011-01-01

After a long period of quiescence in gamma rays, blazar S5 0836+71 (4C71.07) flared in the Spring of 2011. We found only limited multiwavelength coverage of the source. An indication of correlated optical/gamma-ray variability is not surprising for a FSRQ like this one. Radio observations at high frequencies, however, had seen a flare in late 2010, with no apparent related gamma-ray activity. This case seems to differ from the traditional pattern of finding gamma-ray flares during times of rising radio emission.

PD Trafficking of Potato Leaf Roll Virus Movement Protein in Arabidopsis Depends on Site-specific Protein Phosphorylation

PubMed Central

Sonnewald, Uwe

2011-01-01

Many plant viruses encode for specialized movement proteins (MP) to facilitate passage of viral material to and through plasmodesmata (PD). To analyze intracellular trafficking of potato leaf roll virus (PLRV) movement protein (MP17) we performed GFP fusion experiments with distinct deletion variants of MP17. These studies revealed that the C-terminus of MP17 is essential but not sufficient for PD targeting. Interestingly, fusion of GFP to three C-terminal MP17 deletion variants resulted in the accumulation of GFP in chloroplasts. This indicates that MP17 harbors hidden plastid targeting sequences. Previous studies showed that posttranslational protein phosphorylation influences PD targeting of MP and virus spread. Analysis of MP17-derived phospho-peptides by mass spectrometry revealed four phosphorylated serine residues (S71, S79, S137, and S140). Site-directed mutagenesis of S71/S79 and S137/S140 showed that the C-terminal serine residues S137/S140 are dispensable for PD targeting. However, exchange of S71/S79 to A71/A79 abolished PD targeting of the mutated MP17 protein. To mimic phosphorylation of S71/S79 both amino acids were substituted by aspartic acid. The resulting D71/D79 variant of MP17 was efficiently targeted to PD. Further deletion analysis showed that PD targeting of MP17 is dependent on the C-terminus, phosphorylation of S71 and/or S79 and a N-terminal domain. PMID:22645527

Emended description of the family Chromatiaceae, phylogenetic analyses of the genera Alishewanella, Rheinheimera and Arsukibacterium, transfer of Rheinheimera longhuensis LH2-2T to the genus Alishewanella and description of Alishewanella alkalitolerans sp. nov. from Lonar Lake, India.

PubMed

Sisinthy, Shivaji; Chakraborty, Dwaipayan; Adicherla, Harikrishna; Gundlapally, Sathyanarayana Reddy

2017-09-01

Phylogenetic analyses were performed for members of the family Chromatiaceae, signature nucleotides deduced and the genus Alishewanella transferred to Chromatiaceae. Phylogenetic analyses were executed for the genera Alishewanella, Arsukibacterium and Rheinheimera and the genus Rheinheimera is proposed to be split, with the creation of the Pararheinheimera gen. nov. Furthermore, the species Rheinheimera longhuensis, is transferred to the genus Alishewanella as Alishewanella longhuensis comb. nov. Besides, the genera Alishewanella and Rheinheimera are also emended. Strain LNK-7.1 T was isolated from a water sample from the Lonar Lake, India. Cells were Gram-negative, motile rods, positive for catalase, oxidase, phosphatase, contained C 16:0 , C 17:1 ω8c, summed feature3 (C 16:1 ω6c and/or C 16:1 ω7c) and summed feature 8 (C 18:1 ω7c) as major fatty acids, PE and PG as the major lipids and Q-8 as the sole respiratory quinone. Phylogenetic analyses using NJ, ME, ML and Maximum parsimony, based on 16S rRNA gene sequences, identified Alishewanella tabrizica RCRI4 T as the closely related species of strain LNK-7.1 T with a 16S rRNA gene sequence similarity of 98.13%. The DNA-DNA similarity between LNK-7.1 T and the closely related species (A. tabrizica) was only 12.0% and, therefore, strain LNK-7.1 T was identified as a novel species of the genus Alishewanella with the proposed name Alishewanella alkalitolerans sp. nov. In addition phenotypic characteristics confirmed the species status to strain LNK-7.1 T . The type strain of A. alkalitolerans is LNK-7.1 T (LMG 29592 T  = KCTC 52279 T ), isolated from a water sample collected from the Lonar lake, India.

The Association of Recombination Events in the Founding and Emergence of Subgenogroup Evolutionary Lineages of Human Enterovirus 71

PubMed Central

McWilliam Leitch, E. C.; Cabrerizo, M.; Cardosa, J.; Harvala, H.; Ivanova, O. E.; Koike, S.; Kroes, A. C. M.; Lukashev, A.; Perera, D.; Roivainen, M.; Susi, P.; Trallero, G.; Evans, D. J.

2012-01-01

Enterovirus 71 (EV71) is responsible for frequent large-scale outbreaks of hand, foot, and mouth disease worldwide and represent a major etiological agent of severe, sometimes fatal neurological disease. EV71 variants have been classified into three genogroups (GgA, GgB, and GgC), and the latter two are further subdivided into subgenogroups B1 to B5 and C1 to C5. To investigate the dual roles of recombination and evolution in the epidemiology and transmission of EV71 worldwide, we performed a large-scale genetic analysis of isolates (n = 308) collected from 19 countries worldwide over a 40-year period. A series of recombination events occurred over this period, which have been identified through incongruities in sequence grouping between the VP1 and 3Dpol regions. Eleven 3Dpol clades were identified, each specific to EV71 and associated with specific subgenogroups but interspersed phylogenetically with clades of coxsackievirus A16 and other EV species A serotypes. The likelihood of recombination increased with VP1 sequence divergence; mean half-lives for EV71 recombinant forms (RFs) of 6 and 9 years for GgB and GgC overlapped with those observed for the EV-B serotypes, echovirus 9 (E9), E30, and E11, respectively (1.3 to 9.8 years). Furthermore, within genogroups, sporadic recombination events occurred, such as the linkage of two B4 variants to RF-W instead of RF-A and of two C4 variants to RF-H. Intriguingly, recombination events occurred as a founding event of most subgenogroups immediately preceding their lineage expansion and global emergence. The possibility that recombination contributed to their subsequent spread through improved fitness requires further biological and immunological characterization. PMID:22205739

The spatial distribution of vulnerability to the health impacts of flooding in the Mekong Delta, Vietnam.

PubMed

Phung, Dung; Rutherford, Shannon; Dwirahmadi, Febi; Chu, Cordia; Do, Cuong Manh; Nguyen, Thuy; Duong, Nam Chi

2016-06-01

Flooding causes significant public health issues. The Mekong Delta has been considered the region to be the most vulnerable to flooding in Vietnam. This study assessed the spatial vulnerability of the health impacts of flooding in the Mekong Delta region, Vietnam. This study applied a vulnerability assessment framework which was computed as the function of three dimensions: exposure, sensitivity, and adaptive capacity. The indicators for each dimension were derived from the relevant literature, consultations with experts, and data availability. An analytic hierarchy process (AHP) and a principal component analysis (PCA) were used to determine the weight of indicators. Vulnerability indexes (VIs) were then computed for each province. A total of 29 indicators (sensitivity index, 14; adaptive capacity index, 13; and exposure index, 2) were employed to evaluate the vulnerability to the health impacts of flooding at a provincial level. The results of AHP revealed that the highest VIs were found in the Dong Thap and An Giang provinces (VI, 1.948 and 1.574, respectively). VIs were distributed with higher indexes in upstream provinces close to a river than in coastal provinces. PCA generated three components from the 29 indicators, and the VIs computed from the PCA method are in substantial agreement with the AHP method (ICC = 0.71, p < 0.05). The vulnerability to the health impacts of flooding varies from province to province in the Mekong Delta region in Vietnam. Individual plans for health preparedness and adaption to flooding should be developed for each province in the Mekong Delta region.

Forskolin-induced apical membrane insertion of virally expressed, epitope-tagged CFTR in polarized MDCK cells.

PubMed

Howard, M; Jiang, X; Stolz, D B; Hill, W G; Johnson, J A; Watkins, S C; Frizzell, R A; Bruton, C M; Robbins, P D; Weisz, O A

2000-08-01

Channel gating of the cystic fibrosis transmembrane conductance regulator (CFTR) is activated in response to cAMP stimulation. In addition, CFTR activation may also involve rapid insertion of a subapical pool of CFTR into the plasma membrane (PM). However, this issue has been controversial, in part because of the difficulty in distinguishing cell surface vs. intracellular CFTR. Recently, a fully functional, epitope-tagged form of CFTR (M2-901/CFTR) that can be detected immunologically in nonpermeabilized cells was characterized (Howard M, Duvall MD, Devor DC, Dong J-Y, Henze K, and Frizzell RA. Am J Physiol Cell Physiol 269: C1565-C1576, 1995; and Schultz BD, Takahashi A, Liu C, Frizzell RA, and Howard M. Am J Physiol Cell Physiol 273: C2080-C2089, 1997). We have developed replication-defective recombinant adenoviruses that express M2-901/CFTR and used them to probe cell surface CFTR in forskolin (FSK)-stimulated polarized Madin-Darby canine kidney (MDCK) cells. Virally expressed M2-901/CFTR was functional and was readily detected on the apical surface of FSK-stimulated polarized MDCK cells. Interestingly, at low multiplicity of infection, we observed FSK-stimulated insertion of M2901/CFTR into the apical PM, whereas at higher M2-901/CFTR expression levels, no increase in surface expression was detected using indirect immunofluorescence. Immunoelectron microscopy of unstimulated and FSK-stimulated cells confirmed the M2-901/CFTR redistribution to the PM upon FSK stimulation and demonstrates that the apically inserted M2-901/CFTR originates from a population of subapical vesicles. Our observations may reconcile previous conflicting reports regarding the effect of cAMP stimulation on CFTR trafficking.

Erysipelothrix larvae sp. nov., isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae).

PubMed

Bang, Byung-Ho; Rhee, Moon-Soo; Chang, Dong-Ho; Park, Doo-Sang; Kim, Byoung-Chan

2015-02-01

A novel, Gram-stain positive, facultative anaerobic, non-motile and straight to curve rod shaped bacterium, strain LV19(T) was isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus, which was collected from Yeong-dong, Chuncheongbuk-do, South Korea. The colonies of the new isolate were convex, circular, cream white in color and 1-2 mm in diameter after 3 days incubation on Tryptic Soy Agar at 37 °C. Based on the 16S rRNA gene sequence similarity, the new isolate was most closely related to Erysipelothrix inopinata MF-EP02(T), E. rhusiopathiae ATCC 19414 (T) and E. tonsillarum T-305(T) (94.8, 93.8 and 93.7 % similarity, respectively). Strain LV19(T) grew optimally at 37 °C, at pH 8.0 and in the presence of 0.5 % (w/v) NaCl. Oxidase activity and catalase activity were negative. The major cellular fatty acids (>10 %) were C18:2 cis-9,12 (28.9 %), C18:1 cis-9 (22.3 %), C16:0 (22.2 %) and C18:0 (18.5 %). The cell-wall hydrolysates contained ribose as a major sugar. Major polar lipids were phosphatidylglycerol and three unidentified glycolipids. No quinone was detected. The G+C content of the genomic DNA was 36.3 mol%. The levels of DNA-DNA relatedness between strain LV19(T) and all the reference strains were less than 20 %. On the basis of polyphasic evidence from this study, the isolate is considered to represent a novel species of the genus Erysipelothrix, for which the name Erysipelothrix larvae sp. nov. is proposed; the type strain is LV19(T) (=KCTC 33523(T) = DSM 28480(T)).

The Role of Carburization in Gun Barrel Erosion and Cracking

DTIC Science & Technology

1981-07-01

same concentration of active constituents as tests 63 and 64. Test 71 was conducted as an "inert" comparison of tests 63 and 69, since the peak gas...NJ to ^ oo CJl CTJ CJl ( 71 ɟl 3 13 o c bO O U -H nj to C1.-H tD CD 3 P tD O Ci3 X Lp -H u 3 to O C 3 LP...replacing nitrogen with CO. With the exception of the first group of tests, runs 63 through 71 , the total concentration of carbon monoxide and nitrogen

48 CFR 352.237-71 - Crime Control Act-reporting of child abuse.

Code of Federal Regulations, 2010 CFR

2010-10-01

... mental injury, sexual abuse or exploitation, or negligent treatment of a child. (c) Accordingly, any...-reporting of child abuse. 352.237-71 Section 352.237-71 Federal Acquisition Regulations System HEALTH AND... Clauses 352.237-71 Crime Control Act—reporting of child abuse. As prescribed in 337.103-70(b), the...

22 CFR 41.71 - Transit aliens.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Transit aliens. 41.71 Section 41.71 Foreign... NATIONALITY ACT, AS AMENDED Transit Aliens § 41.71 Transit aliens. (a) Transit aliens—general. An alien is classifiable as a nonimmigrant transit alien under INA 101(a) (15) (C) if the consular officer is satisfied...

22 CFR 41.71 - Transit aliens.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Transit aliens. 41.71 Section 41.71 Foreign... NATIONALITY ACT, AS AMENDED Transit Aliens § 41.71 Transit aliens. (a) Transit aliens—general. An alien is classifiable as a nonimmigrant transit alien under INA 101(a) (15) (C) if the consular officer is satisfied...

22 CFR 41.71 - Transit aliens.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Transit aliens. 41.71 Section 41.71 Foreign... NATIONALITY ACT, AS AMENDED Transit Aliens § 41.71 Transit aliens. (a) Transit aliens—general. An alien is classifiable as a nonimmigrant transit alien under INA 101(a) (15) (C) if the consular officer is satisfied...

22 CFR 41.71 - Transit aliens.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Transit aliens. 41.71 Section 41.71 Foreign... NATIONALITY ACT, AS AMENDED Transit Aliens § 41.71 Transit aliens. (a) Transit aliens—general. An alien is classifiable as a nonimmigrant transit alien under INA 101(a) (15) (C) if the consular officer is satisfied...

22 CFR 41.71 - Transit aliens.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Transit aliens. 41.71 Section 41.71 Foreign... NATIONALITY ACT, AS AMENDED Transit Aliens § 41.71 Transit aliens. (a) Transit aliens—general. An alien is classifiable as a nonimmigrant transit alien under INA 101(a) (15) (C) if the consular officer is satisfied...

Tropical Engineering. Design Manual-11.1.

DTIC Science & Technology

1980-03-01

fication TS-15250 for cold piping below 35 degrees F. Cellular glass 11.1-75 insulation only shall be used. Flexible unicellular insulation shall not be...11.1-71 2. Materials .........o......................................11.1-71 a. Underground Water Piping ...11.1-71 b. Protective Coating for Copper Tubing .................11.1-71 c. Above-ground Piping ................................. 11.1

32 CFR 536.71 - Fund sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true Fund sources. 536.71 Section 536.71 National... UNITED STATES Investigation and Processing of Claims § 536.71 Fund sources. (a) 31 U.S.C. 1304 sets forth the type and limits of claims payable out of the Judgment Fund. Only final payments that are not...

32 CFR 536.71 - Fund sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 3 2011-07-01 2009-07-01 true Fund sources. 536.71 Section 536.71 National... UNITED STATES Investigation and Processing of Claims § 536.71 Fund sources. (a) 31 U.S.C. 1304 sets forth the type and limits of claims payable out of the Judgment Fund. Only final payments that are not...

Doppler Compensation for Airborne Non-Side-Looking Phased-Array Radar

DTIC Science & Technology

2015-09-01

Box 1500 Edinburgh South Australia 5111 Australia Telephone: 1300 333 362 Fax: (08) 7389 6567 © Commonwealth of Australia 2013 AR-016...Security and ISR Division Dr Yunhan Dong received his Bachelor and Master degrees in 1980s in China and PhD in 1995 at UNSW, Australia , all in...waveform length, 0λ 0.25 m Bandwidth of LFM 5 MHz Sampling rate 10 MHz Number of array elements, N 25 Number of pulses in a CPI, M 31 Antenna

A Method to Measure the Amount of Battlefield Situation Information

DTIC Science & Technology

2014-06-01

log)( 20 atS  3.2 Measurement of trends information Kierkegaard once said “Life can only be understood backwards, but it must be lived forwards” [8...Towards a theory of s 10 11 , “The Journals of Soren Kierkegaard ”, A selection and translated by Alexander chun, “Formal Description of Command...and 37(2), pp32-64, 1995. [8] Kierkegaard , Soren – Dru , Oxford: Oxford University Press (1938). pp465 [9] ZHOU Dao-an, ZHANG Dong-ge, CHANG Shu

Electrical Characteristics of GaAs MESFET Fabrication by Ion Implantation of Si or Se

DTIC Science & Technology

1993-10-04

only the z-component of the polarization vector is non-zero, given by - dao . Since all the stress tensors are independent of z, the effective charge...GaAs," Stanford University, 1990. [381 Jong-Lam Lee , Jin Sup Kim, Hyung Moo Park, and Dong Sung Ma, "Depth Pro- files on Ion Implantation Induced Vacancy...February 1990. [40] Yao- Tsung Tsai and Timothy A. Grotjohn, "Source and Drain Resistance Studies of Short Channel MESFET’s Using Two-Dimensional Device

A System for Fast Navigation of Autonomous Vehicles

DTIC Science & Technology

1991-09-01

AD-A243 523 4, jj A System for Fast Navigation of Autonomous Vehicles Sanjiv Singh, Dai Feng, Paul Keller, Gary Shaffer, Wen Fan Shi, Dong Hun Shin...FUNDING NUMBERS A System for Fast Navigation of Autonomous Vehicles 6. AUTHOR(S) S. Singh, D. Feng, P. Keller, G. Shaffer, W.F. Shi, D.H. Shin, J. West...common in the control of autonomous vehicles to establish the necessary kinematic models but to ignore an explicit representation of the vehicle dynamics

Characterizing SHP2 as a Novel Therapeutic Target in Breast Cancer

DTIC Science & Technology

2012-02-01

50(12), 2339-56. doi:10.1021/bi1014453 Zhang, X., He, Y., Liu, S., Yu, Z., Jiang, Z. X., Yang, Z., Dong, Y., et al. (2010). Salicylic Acid Based...Overall, what we have found so far has been surprising, with the data suggesting that the presence of a -2 acidic residue in the substrate peptide...helped to refine the initial docking poses, with the initial hypothesis being that the pY -1 and -4 position acidic amino acids will bind a pair of

Enhancement of Radiation Therapy in Prostate Cancer by DNA-PKcs Inhibitor

DTIC Science & Technology

2013-07-01

Dawen Zhao, Masaya Takahashi, Timothy Dobin, Leah Gandee, Timothy D. Solberg, Amyn A. Habib and Debabrata Saha; International Journal of Oncology, 42...5] Kong Z, Xie D, Boike T, Raghavan P, Burma S, Chen DJ, Habib AA, Chakraborty A, Hsieh JT, and Saha D (2010). Downregulation of human DAB2IP gene...study. Phys Med Biol 56: 1243-1258, 2011. 4. De Crevoisier R, Tucker SL , Dong L, et al: Increased risk of biochemical and local failure in patients

China and Proliferation of Weapons of Mass Destruction and Missiles: Policy Issues

DTIC Science & Technology

2007-05-09

medium-range ballistic missile (MRBM), which is based on the North Korean No Dong missile. U.S. intelligence was said to suspect that China Poly Ventures...stories, the DCI reported in August 2000 that, besides North Korean help, PRC entities provided “increased assistance” to Pakistan’s ballistic missile...development of the Shahab-3 and Shahab-4 MRBMs (with ranges, respectively, of about 800 mi. and 1,250 mi.). Over 100 PRC and North Korean experts worked there

China and Proliferation of Weapons of Mass Destruction and Missiles: Policy Issues

DTIC Science & Technology

2007-01-31

based on the North Korean No Dong missile. U.S. intelligence was said to suspect that China Poly Ventures Company delivered, perhaps in 1999, U.S.-made...besides North Korean help, PRC entities provided “increased assistance” to Pakistan’s ballistic missile program in the second half of 1999. Also...the Shahab-3 and Shahab-4 MRBMs (with ranges, respectively, of about 800 mi. and 1,250 mi.). Over 100 PRC and North Korean experts worked there

The Impact of US Forces in Korea

DTIC Science & Technology

1987-01-01

190-193. 13. Kim Chum, The Korean War, pp. 187-188. 14. Chu Yong -bok, "How Longing," Dong-A Ilbo, July 10, 1952. p. 2. Chu was a former People’s Army...those who provided comments and suggestions were Brigadier General Kim Jung Whan, formerly colonel, Combined Forces Com- mand, ROK/US; Brigadier General...Korean Defense College faculty; and two class- mates at the Korean Military Academy (class of 1965), Colonel Park Yong Ok, of the Korean Defense

Microbial Reduction of Structural Fe3+ in Nontronite by a Thermophilic Bacterium and its Role in Promoting the Smectite to Illite Reaction

DTIC Science & Technology

2007-01-01

role in promoting the smectite to Hike reaction GENGXIN ZHANG,’ HAIUANG DONG, 1 * JINWOOK KIM,2 AND D.D. EBERL3 ’Department of Geology, Miami...Geological Survey, Boulder, Colorado 80303, USA. ABSTRACT The illitization process of Fe-rich smectite (nontronite NAu-2) promoted by microbial reduction of...layers of illite/ smectite or highly charged smectite were detected under other conditions. The morphology of biogenic illite evolved from lath and flake

Command History. 1967. Volume 1. Sanitized

DTIC Science & Technology

1967-01-01

the Politburo of the Lao Dong Party. This top political control center included Le Duan, General Vo Nguyen Glap, Truong Chinh , Le Duc Tho, and until...the support area Naval Support Activity (NSA) Da Nang’s vital intra- coastal trans -shipment route from the deep water port at Da Nang northward to...under the energetic guidance of MG Nguyen Duc Thang, reviewed PAGE 6 OF 1340 PAGES I% the entire process of RD and set to the task of revitalizing the

Through the Eyes of the Dragon: Vietnamese Communist Grand Strategy during the Second Indochina War

DTIC Science & Technology

1992-04-01

adaptation of revolutionary war in their struggle against the French was documented by two major figures. Truong Chinh , secretary general of the...the living embodiment to his own people--and to the world--of their revolution." 2 3 -23- Ho and his lieutenants, Le Duan, Le Duc Tho, Pham Van Dong...increased aid from their main supporters. In January 1964, Le Duan and Le Duc Tho travelled to Moscow to explain the situation and ask for more

A Simple Hierarchical Pooling Data Structure for Loop Closure

DTIC Science & Technology

2016-10-16

ticated agglomerative schemes at a fraction of the effort. 1.1 Related work Loop closure is a key component in robotic mapping (SLAM) [37], autonomous...appearance-only slam-fab-map 2.0. In: Robotics : Science and Systems. vol. 5. Seattle, USA (2009) 7. Dong, J., Soatto, S.: Domain size pooling in local...detection with bags of binary words. In: Intelligent Robots and Systems (IROS), 2011 IEEE/RSJ Intl. Conf. on. pp. 51–58. IEEE (2011) 9. Geiger, A

Why Did the Strategic Hamlet Program Fail

DTIC Science & Technology

1990-01-01

Diem’s horoscope was a lucky one; the conviction was clinched by the unreserved support, moral and financial, of the US, from which so many urban...pointed "the way of duty which Is also the way of true liberty." 2 2 This concept of duty to the state sulted the Confucian view of Diem and Nhu: and. with...leadership of the Vietnamese Workers Party. Douglas Pike called the NLF a true Communist-front organization ano quoted a Lao Dong (Workers) Party memorandum

China 2015: U.S. National Strategic Policy Now and Then

DTIC Science & Technology

1996-06-05

even rosy outlook are from Gregor (UC Berkely ) and Kelly (Pacific CSIS), who firmly believe that China currently views us as an enemy who will...Xinxian, Sanjiu Enterprise Group, the PLA’s first transnational corporation , Boston Globe, May 17, 1993, Bickford, 460. 63Dadush, Uri and Dong He...34China: A New Power in World Trade," Finance and Development, Vol. 32, June 1995, 37. 64Knocking Knees," The Economist, 16 March 1996, 40. 65 Harry

Seedling development and evaluation of genetic stability of cryopreserved Dendrobium hybrid mature seeds.

PubMed

Galdiano, Renato Fernandes; de Macedo Lemos, Eliana Gertrudes; de Faria, Ricardo Tadeu; Vendrame, Wagner Aparecido

2014-03-01

Vitrification, a simple, fast, and recommended cryopreservation method for orchid germplasm conservation, was evaluated for Dendrobium hybrid "Dong Yai" mature seeds. The genetic stability of regenerated seedlings was also evaluated using flow cytometry. Mature seeds from this hybrid were submitted to plant vitrification solution (PVS2) for 0, 0.5, 1, 2, 3, 4, 5, or 6 h at 0 °C. Subsequently, they were plunged into liquid nitrogen (LN) at -196 °C for 1 h and recovered in half-strength Murashige and Skoog culture medium (1/2 MS), and seed germination was evaluated after 30 days. Seeds directly submitted to LN did not germinate after cryopreservation. Seeds treated with PVS2 between 1 and 3 h presented the best germination (between 51 and 58%), although longer exposure to PVS2 returned moderated germination (39%). Germinated seeds were further subcultured in P-723 culture medium and developed whole seedlings in vitro after 180 days, with no abnormal characteristics, diseases, or nutritional deficiencies. Seedlings were successfully acclimatized under greenhouse conditions with over 80% survival. Flow cytometry analysis revealed no chromosomal changes on vitrified seedlings, as well as seedlings germinated from the control treatment (direct exposure to LN). These findings indicate that vitrification is a feasible and safe germplasm cryopreservation method for commercial Dendrobium orchid hybrid conservation.

Enterovirus 71 infection in children with hand, foot, and mouth disease in Shanghai, China: epidemiology, clinical feature and diagnosis.

PubMed

Wang, Ying; Zou, Gang; Xia, Aimei; Wang, Xiangshi; Cai, Jiehao; Gao, Qianqian; Yuan, Shilin; He, Guimei; Zhang, Shuyi; Zeng, Mei; Altmeyer, Ralf

2015-06-03

In 2012 a large outbreak of hand, foot, and mouth disease (HFMD) widely spread over China, causing more than 2 million cases and 567 deaths. Our purpose was to characterize the major pathogens responsible for the 2012 HFMD outbreak and analyze the genetic characterization of the enterovirus 71 (EV71) strains in Shanghai; also, to analyze the dynamic patterns of neutralizing antibody (NAb) against EV71 and evaluate the diagnostic value of several methods for clinical detection of EV71. Clinical samples including stool, serum and CSF were collected from 396 enrolled HFMD inpatients during the peak seasons in 2012. We analyzed the molecular epidemiology, clinical feature, and diagnostic tests of EV71 infection. EV71 was responsible for 60.35 % of HFMD inpatients and 88.46 % of severe cases. The circulating EV71 strains belonged to subgenogroup C4a. The nucleotide sequences of VP1 between severe cases and uncomplicated cases shared 99.2 ~ 100 % of homology. Among 218 cases with EV71 infection, 211 (96.79 %) serum samples showed NAb positive against EV71 and NAb titer reached higher level 3 days after disease onset. Of 92 cases with EV71-associated meningitis or encephalitis, 5 (5.43 %) of 92 had EV71 RNA detected in CSF samples. The blood anti-EV71 IgM assay showed a sensitivity of 93.30 % and a specificity of 50 %. EV71 C4a remained the predominant subgenotype circulating in Shanghai. The severity of the EV71 infection is not associated with the virulence determinants in VP1. RT-PCR together with IgM detection can enhance the early diagnosis of severe EV71-associated HFMD.

Is the Dark Side of the Moon Ever Illuminated by the Sun? (Breton Title: O Lado Escuro da Lua Nunca Apanha Sol?) El Lado Oscuro de la Luna Nunca Toma Sol?

NASA Astrophysics Data System (ADS)

Giovannini, Odilon; Pellenz, Daiana; Catelli, Francisco

2014-07-01

This work presents the elaboration of an answer to the question "Is the dark side of the moon ever illuminated by the sun?", in which issues related to the students' conceptions, how they arise, and the core elements that constitute a "good answer" are considered. These issues are initially elaborated from a literature review of the research in the field; then, and also based on this review, a didactic model that involves the relative motions of the Earth - Moon - Sun system is proposed. A description of the application of this model in a learning environment within middle school students is described. This work confirms the students' initial concepts described in the literature; on the other hand, the questions asked by students during the activity provide evidence that meaningful learning, at least to some degree, has occurred. Este trabalho apresenta a elaboração de uma resposta à pergunta "O lado escuro da Lua nunca apanha sol?" na qual são consideradas questões relacionadas às concepções iniciais dos alunos, de onde elas surgem e os elementos centrais que constituem uma "boa resposta". Essas questões são tratadas, inicialmente, a partir de uma revisão da literatura sobre pesquisas na área e, em seguida, também com base nessa revisão, um objeto modelo didático que envolve os movimentos relativos do sistema Terra - Lua - Sol é proposto. Uma descrição detalhada da aplicação deste modelo em um ambiente de aprendizagem com alunos do ensino fundamental é relatada. As concepções iniciais apresentadas na literatura se confirmam e os questionamentos feitos pelos estudantes por ocasião da aplicação do modelo fornecem indícios de que uma aprendizagem significativa, pelo menos em algum grau, ocorreu. El artículo se presenta el desarrollo de una respuesta a la pregunta "¿El lado oscuro de la luna nunca toma sol?", en la que se consideran algunas cuestiones vinculadas que se refieren a las concepciones iniciales de los estudiantes, dónde surgen y los elementos básicos que componen una "respuesta correcta". Estas cuestiones se tratan inicialmente a partir de una revisión bibliográfica de la investigación en el área; seguidamente, y también sobre la base de ésta revisión, se propone un modelo didáctico que ejemplifica los movimientos relativos del sistema Tierra - Luna - Sol. Se describe a continuación una aplicación de este modelo en un entorno de aprendizaje con alumnos del ciclo primario. Los conceptos iniciales presentados en la literatura se confirman y las cuestiones planteadas por los estudiantes durante la implementación del modelo proporcionan evidencia de que se ha producido un aprendizaje significativo, al menos en cierta medida.

Structure-activity relationship study of peptidomimetic aldehydes as enterovirus 71 3C protease inhibitors.

PubMed

Zhai, Yangyang; Ma, Yuying; Ma, Fei; Nie, Quandeng; Ren, Xuejiao; Wang, Yaxin; Shang, Luqing; Yin, Zheng

2016-11-29

A series of peptidomimetic aldehydes were designed, synthesized, and evaluated for their biochemical activity against 3C protease (3C pro ) and anti-enterovirus 71 (EV71) activity in vitro. Molecular docking revealed that 5s (IC 50  = 0.22 ± 0.07 μM, EC 50  = 0.18 ± 0.05 μM) could bind well to the active site of EV71 3C pro , which was consistent with the biological data compared to reference 5a (IC 50  = 0.54 ± 0.02 μM, EC 50  = 0.26 ± 0.07 μM). Structure and relationship study led to the discovery of aldehyde 5x (IC 50  = 0.10 ± 0.02 μM, EC 50  = 0.11 ± 0.07 μM), which exhibited the most potent 3C pro inhibitory and antiviral activity. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Peptidyl aldehyde NK-1.8k suppresses enterovirus 71 and enterovirus 68 infection by targeting protease 3C.

PubMed

Wang, Yaxin; Yang, Ben; Zhai, Yangyang; Yin, Zheng; Sun, Yuna; Rao, Zihe

2015-05-01

Enterovirus (EV) is one of the major causative agents of hand, foot, and mouth disease in the Pacific-Asia region. In particular, EV71 causes severe central nervous system infections, and the fatality rates from EV71 infection are high. Moreover, an outbreak of respiratory illnesses caused by an emerging EV, EV68, recently occurred among over 1,000 young children in the United States and was also associated with neurological infections. Although enterovirus has emerged as a considerable global public health threat, no antiviral drug for clinical use is available. In the present work, we screened our compound library for agents targeting viral protease and identified a peptidyl aldehyde, NK-1.8k, that inhibits the proliferation of different EV71 strains and one EV68 strain and that had a 50% effective concentration of 90 nM. Low cytotoxicity (50% cytotoxic concentration, >200 μM) indicated a high selective index of over 2,000. We further characterized a single amino acid substitution inside protease 3C (3C(pro)), N69S, which conferred EV71 resistance to NK-1.8k, possibly by increasing the flexibility of the substrate binding pocket of 3C(pro). The combination of NK-1.8k and an EV71 RNA-dependent RNA polymerase inhibitor or entry inhibitor exhibited a strong synergistic anti-EV71 effect. Our findings suggest that NK-1.8k could potentially be developed for anti-EV therapy. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

KCNE1 remodels the voltage sensor of Kv7.1 to modulate channel function.

PubMed

Wu, Dick; Pan, Hua; Delaloye, Kelli; Cui, Jianmin

2010-12-01

The KCNE1 auxiliary subunit coassembles with the Kv7.1 channel and modulates its properties to generate the cardiac I(Ks) current. Recent biophysical evidence suggests that KCNE1 interacts with the voltage-sensing domain (VSD) of Kv7.1. To investigate the mechanism of how KCNE1 affects the VSD to alter the voltage dependence of channel activation, we perturbed the VSD of Kv7.1 by mutagenesis and chemical modification in the absence and presence of KCNE1. Mutagenesis of S4 in Kv7.1 indicates that basic residues in the N-terminal half (S4-N) and C-terminal half (S4-C) of S4 are important for stabilizing the resting and activated states of the channel, respectively. KCNE1 disrupts electrostatic interactions involving S4-C, specifically with the lower conserved glutamate in S2 (Glu(170) or E2). Likewise, Trp scanning of S4 shows that mutations to a cluster of residues in S4-C eliminate current in the presence of KCNE1. In addition, KCNE1 affects S4-N by enhancing MTS accessibility to the top of the VSD. Consistent with the structure of Kv channels and previous studies on the KCNE1-Kv7.1 interaction, these results suggest that KCNE1 alters the interactions of S4 residues with the surrounding protein environment, possibly by changing the protein packing around S4, thereby affecting the voltage dependence of Kv7.1. Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

45 CFR 96.71 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Definitions. 96.71 Section 96.71 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION BLOCK GRANTS Social Services Block Grants § 96.71 Definitions. (a) Section 2005 (a)(2) and (a)(5) (42 U.S.C. 1397d (a)(2) and (a)(5)) of the Social...

Genetic Characterization of Enterovirus A71 Circulating in Africa.

PubMed

Fernandez-Garcia, Maria Dolores; Volle, Romain; Joffret, Marie-Line; Sadeuh-Mba, Serge Alain; Gouandjika-Vasilache, Ionela; Kebe, Ousmane; Wiley, Michael R; Majumdar, Manasi; Simon-Loriere, Etienne; Sakuntabhai, Anavaj; Palacios, Gustavo; Martin, Javier; Delpeyroux, Francis; Ndiaye, Kader; Bessaud, Maël

2018-04-01

We analyzed whole-genome sequences of 8 enterovirus A71 isolates (EV-A71). We confirm the circulation of genogroup C and the new genogroup E in West Africa. Our analysis demonstrates wide geographic circulation and describes genetic exchanges between EV-A71 and autochthonous EV-A that might contribute to the emergence of pathogenic lineages.

Genetic Characterization of Enterovirus A71 Circulating in Africa

PubMed Central

Volle, Romain; Joffret, Marie-Line; Sadeuh-Mba, Serge Alain; Gouandjika-Vasilache, Ionela; Kebe, Ousmane; Wiley, Michael R.; Majumdar, Manasi; Simon-Loriere, Etienne; Sakuntabhai, Anavaj; Palacios, Gustavo; Martin, Javier; Delpeyroux, Francis; Ndiaye, Kader; Bessaud, Maël

2018-01-01

We analyzed whole-genome sequences of 8 enterovirus A71 isolates (EV-A71). We confirm the circulation of genogroup C and the new genogroup E in West Africa. Our analysis demonstrates wide geographic circulation and describes genetic exchanges between EV-A71 and autochthonous EV-A that might contribute to the emergence of pathogenic lineages. PMID:29553325

45 CFR 96.71 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 1 2012-10-01 2012-10-01 false Definitions. 96.71 Section 96.71 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION BLOCK GRANTS Social Services Block Grants § 96.71 Definitions. (a) Section 2005 (a)(2) and (a)(5) (42 U.S.C. 1397d (a)(2) and (a)(5)) of the Social...

45 CFR 96.71 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 1 2014-10-01 2014-10-01 false Definitions. 96.71 Section 96.71 Public Welfare Department of Health and Human Services GENERAL ADMINISTRATION BLOCK GRANTS Social Services Block Grants § 96.71 Definitions. (a) Section 2005 (a)(2) and (a)(5) (42 U.S.C. 1397d (a)(2) and (a)(5)) of the Social...

45 CFR 96.71 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 1 2013-10-01 2013-10-01 false Definitions. 96.71 Section 96.71 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION BLOCK GRANTS Social Services Block Grants § 96.71 Definitions. (a) Section 2005 (a)(2) and (a)(5) (42 U.S.C. 1397d (a)(2) and (a)(5)) of the Social...

45 CFR 96.71 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Definitions. 96.71 Section 96.71 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION BLOCK GRANTS Social Services Block Grants § 96.71 Definitions. (a) Section 2005 (a)(2) and (a)(5) (42 U.S.C. 1397d (a)(2) and (a)(5)) of the Social...

The Clinical Correlation of Regulatory T Cells and Cyclic Adenosine Monophosphate in Enterovirus 71 Infection

PubMed Central

Wang, Shih-Min; Chen, I-Chun; Liao, Yu-Ting; Liu, Ching-Chuan

2014-01-01

Background Brainstem encephalitis (BE) and pulmonary edema (PE) are notable complications of enterovirus 71 (EV71) infection. Objective This study investigated the immunoregulatory characterizations of EV71 neurological complications by disease severity and milrinone treatment. Study Design Patients <18 years with virologically confirmed EV71 infections were enrolled and divided into 2 groups: the hand, foot, and mouth disease (HFMD) or BE group, and the autonomic nervous system (ANS) dysregulation or PE group. Cytokine and cyclic adenosine monophosphate (cAMP) levels, and the regulatory T cell (Tregs) profiles of the patients were determined. Results Patients with ANS dysregulation or PE exhibited significantly low frequency of CD4+CD25+Foxp3+ and CD4+Foxp3+ T cells compared with patients with HFMD or BE. The expression frequency of CD4−CD8− was also significantly decreased in patients with ANS dysregulation or PE. Among patients with ANS dysregulation or PE, the expression frequency of CD4+Foxp3+ increased markedly after milrinone treatment, and was associated with reduction of plasma levels IL-6, IL-8 and IL-10. Plasma concentrations of cAMP were significantly decreased in patients with ANS dysregulation or PE compared with patients with HFMD or BE; however, cAMP levels increased after milrinone treatment. Conclusions These findings suggested decreased different regulatory T populations and cAMP expression correlate with increased EV71 disease severity. Improved outcome after milrinone treatment may associate with increased regulatory T populations, cAMP expression and modulation of cytokines levels. PMID:25010330

Development of a sensitive and specific epitope-blocking ELISA for universal detection of antibodies to human enterovirus 71 strains.

PubMed

He, Fang; Kiener, Tanja K; Lim, Xiao Fang; Tan, Yunrui; Raj, Kattur Venkatachalam Ashok; Tang, Manli; Chow, Vincent T K; Chen, Qingfeng; Kwang, Jimmy

2013-01-01

Human Enterovirus 71 (EV71) is a common cause of hand, foot and mouth disease (HFMD) in young children. It is often associated with severe neurological diseases and mortalities in recent outbreaks across the Asia Pacific region. Currently, there is no efficient universal antibody test available to detect EV71 infections. In the present study, an epitope-blocking ELISA was developed to detect specific antibodies to human EV71 viruses in human or animal sera. The assay relies on a novel monoclonal antibody (Mab 1C6) that specifically binds to capsid proteins in whole EV71 viruses without any cross reaction to any EV71 capsid protein expressed alone. The sensitivity and specificity of the epitope-blocking ELISA for EV71 was evaluated and compared to microneutralization using immunized animal sera to multiple virus genotypes of EV71 and coxsackieviruses. Further, 200 serum sample from human individuals who were potentially infected with EV71 viruses were tested in both the blocking ELISA and microneutralization. Results indicated that antibodies to EV71 were readily detected in immunized animals or human sera by the epitope blocking ELISA whereas specimens with antibodies to other enteroviruses yielded negative results. This assay is not only simpler to perform but also shows higher sensitivity and specificity as compared to microneutralization. The epitope-blocking ELISA based on a unique Mab 1C6 provided highly sensitive and 100% specific detection of antibodies to human EV71 viruses in human sera.

Molecular Chaperone Hsp70/Hsp90 Prepares the Mitochondrial Outer Membrane Translocon Receptor Tom71 for Preprotein Loading

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Jingzhi; Qian, Xinguo; Hu, Junbin

2010-11-03

The preproteins targeted to the mitochondria are transported through the translocase of the outer membrane complex. Tom70/Tom71 is a major surface receptor of the translocase of the outer membrane complex for mitochondrial preproteins. The preproteins are escorted to Tom70/Tom71 by molecular chaperones Hsp70 and Hsp90. Here we present the high resolution crystal structures of Tom71 and the protein complexes between Tom71 and the Hsp70/Hsp90 C terminus. The crystal structures indicate that Tom70/Tom71 may exhibit two distinct states. In the closed state, the N-terminal domain of Tom70/Tom71 partially blocks the preprotein-binding pocket. In the open state, the N-terminal domain moves away,more » and the preprotein-binding pocket is fully exposed. The complex formation between the C-terminal EEVD motif of Hsp70/Hsp90 and Tom71 could lock Tom71 in the open state where the preprotein-binding pocket of Tom71 is ready to receive preproteins. The interactions between Hsp70/Hsp90 and Tom71 N-terminal domain generate conformational changes that may increase the volume of the preprotein-binding pocket. The complex formation of Hsp70/Hsp90 and Tom71 also generates significant domain rearrangement within Tom71, which may position the preprotein-binding pocket closer to Hsp70/Hsp90 to facilitate the preprotein transfer from the molecular chaperone to Tom71. Therefore, molecular chaperone Hsp70/Hsp90 may function to prepare the mitochondrial outer membrane receptor Tom71 for preprotein loading.« less

Enterovirus 71 Inhibits Pyroptosis through Cleavage of Gasdermin D

PubMed Central

Lei, Xiaobo; Zhang, Zhenzhen; Xiao, Xia; Qi, Jianli

2017-01-01

ABSTRACT Enterovirus 71 (EV71) can cause hand-foot-and-mouth disease (HFMD) in young children. Severe infection with EV71 can lead to neurological complications and even death. However, the molecular basis of viral pathogenesis remains poorly understood. Here, we report that EV71 induces degradation of gasdermin D (GSDMD), an essential component of pyroptosis. Remarkably, the viral protease 3C directly targets GSDMD and induces its cleavage, which is dependent on the protease activity. Further analyses show that the Q193-G194 pair within GSDMD is the cleavage site of 3C. This cleavage produces a shorter N-terminal fragment spanning amino acids 1 to 193 (GSDMD1–193). However, unlike the N-terminal fragment produced by caspase-1 cleavage, this fragment fails to trigger cell death or inhibit EV71 replication. Importantly, a T239D or F240D substitution abrogates the activity of GSDMD consisting of amino acids 1 to 275 (GSDMD1–275). This is correlated with the lack of pyroptosis or inhibition of viral replication. These results reveal a previously unrecognized strategy for EV71 to evade the antiviral response. IMPORTANCE Recently, it has been reported that GSDMD plays a critical role in regulating lipopolysaccharide and NLRP3-mediated interleukin-1β (IL-1β) secretion. In this process, the N-terminal domain of p30 released from GSDMD acts as an effector in cell pyroptosis. We show that EV71 infection downregulates GSDMD. EV71 3C cleaves GSDMD at the Q193-G194 pair, resulting in a truncated N-terminal fragment disrupted for inducing cell pyroptosis. Notably, GSDMD1–275 (p30) inhibits EV71 replication whereas GSDMD1–193 does not. These results reveal a new strategy for EV71 to evade the antiviral response. PMID:28679757

Enterovirus 71 Inhibits Pyroptosis through Cleavage of Gasdermin D.

PubMed

Lei, Xiaobo; Zhang, Zhenzhen; Xiao, Xia; Qi, Jianli; He, Bin; Wang, Jianwei

2017-09-15

Enterovirus 71 (EV71) can cause hand-foot-and-mouth disease (HFMD) in young children. Severe infection with EV71 can lead to neurological complications and even death. However, the molecular basis of viral pathogenesis remains poorly understood. Here, we report that EV71 induces degradation of gasdermin D (GSDMD), an essential component of pyroptosis. Remarkably, the viral protease 3C directly targets GSDMD and induces its cleavage, which is dependent on the protease activity. Further analyses show that the Q193-G194 pair within GSDMD is the cleavage site of 3C. This cleavage produces a shorter N-terminal fragment spanning amino acids 1 to 193 (GSDMD 1-193 ). However, unlike the N-terminal fragment produced by caspase-1 cleavage, this fragment fails to trigger cell death or inhibit EV71 replication. Importantly, a T239D or F240D substitution abrogates the activity of GSDMD consisting of amino acids 1 to 275 (GSDMD 1-275 ). This is correlated with the lack of pyroptosis or inhibition of viral replication. These results reveal a previously unrecognized strategy for EV71 to evade the antiviral response. IMPORTANCE Recently, it has been reported that GSDMD plays a critical role in regulating lipopolysaccharide and NLRP3-mediated interleukin-1β (IL-1β) secretion. In this process, the N-terminal domain of p30 released from GSDMD acts as an effector in cell pyroptosis. We show that EV71 infection downregulates GSDMD. EV71 3C cleaves GSDMD at the Q193-G194 pair, resulting in a truncated N-terminal fragment disrupted for inducing cell pyroptosis. Notably, GSDMD 1-275 (p30) inhibits EV71 replication whereas GSDMD 1-193 does not. These results reveal a new strategy for EV71 to evade the antiviral response. Copyright © 2017 American Society for Microbiology.

Quanty4RIXS: a program for crystal field multiplet calculations of RIXS and RIXS-MCD spectra using Quanty.

PubMed

Zimmermann, Patric; Green, Robert J; Haverkort, Maurits W; de Groot, Frank M F

2018-05-01

Some initial instructions for the Quanty4RIXS program written in MATLAB ® are provided. The program assists in the calculation of 1s 2p RIXS and 1s 2p RIXS-MCD spectra using Quanty. Furthermore, 1s XAS and 2p 3d RIXS calculations in different symmetries can also be performed. It includes the Hartree-Fock values for the Slater integrals and spin-orbit interactions for several 3d transition metal ions that are required to create the .lua scripts containing all necessary parameters and quantum mechanical definitions for the calculations. The program can be used free of charge and is designed to allow for further adjustments of the scripts. open access.

48 CFR 1552.215-71 - Evaluation factors for award.

Code of Federal Regulations, 2010 CFR

2010-10-01

... for technical acceptability evaluation: (c) Factors for past performance evaluation (optional): (End... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Evaluation factors for award. 1552.215-71 Section 1552.215-71 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION...

Effect of simultaneous therapy of arthrocentesis and occlusal splints on temporomandibular disorders: anterior disc displacement without reduction

PubMed Central

Lee, Hye-Sung; Baek, Hyun-Su; Song, Dong-Suk; Kim, Hee-Chul; Kim, Hyo-Geun; Kim, Bok-Joo; Kim, Myung-Soo; Shin, Sang-Hoon; Jung, Sung-Hee

2013-01-01

Objectives This study sought to evaluate the effect of simultaneous application of arthrocentesis and occlusal splint. Materials and Methods A retrospective study of 43 patients (3 males, 40 females) whose symptoms had improved was conducted at the Department of Oral and Maxillofacial Surgery, Dong-A University Hospital between 2008 and 2010. Subjects were divided into three groups: Group A (17 patients with arthrocentesis and occlusal splints simultaneously applied), Group B (13 patients whose symptoms did not improve with occlusal splints, undergoing arthrocentesis after occlusal splint use for 8 weeks), and Group C (13 patients that only used occlusal splints). We compared these groups in maximum comfortable opening (MCO) and the visual analogue scale of pain and noise. Follow-up was performed at 1 week, 1 month, 3 months, and 6 months. Results The improvement of symptoms was noted in all three groups, but Group A had a quicker improvement than the other groups, in terms of pain reduction and MCO increases. Conclusion The simultaneous application of arthrocentesis and occlusal splints can reduce patient discomfort more quickly. PMID:24471012

Effect of simultaneous therapy of arthrocentesis and occlusal splints on temporomandibular disorders: anterior disc displacement without reduction.

PubMed

Lee, Hye-Sung; Baek, Hyun-Su; Song, Dong-Suk; Kim, Hee-Chul; Kim, Hyo-Geun; Kim, Bok-Joo; Kim, Myung-Soo; Shin, Sang-Hoon; Jung, Sung-Hee; Kim, Chul-Hoon

2013-02-01

This study sought to evaluate the effect of simultaneous application of arthrocentesis and occlusal splint. A retrospective study of 43 patients (3 males, 40 females) whose symptoms had improved was conducted at the Department of Oral and Maxillofacial Surgery, Dong-A University Hospital between 2008 and 2010. Subjects were divided into three groups: Group A (17 patients with arthrocentesis and occlusal splints simultaneously applied), Group B (13 patients whose symptoms did not improve with occlusal splints, undergoing arthrocentesis after occlusal splint use for 8 weeks), and Group C (13 patients that only used occlusal splints). We compared these groups in maximum comfortable opening (MCO) and the visual analogue scale of pain and noise. Follow-up was performed at 1 week, 1 month, 3 months, and 6 months. The improvement of symptoms was noted in all three groups, but Group A had a quicker improvement than the other groups, in terms of pain reduction and MCO increases. The simultaneous application of arthrocentesis and occlusal splints can reduce patient discomfort more quickly.

Improved version of a binding energy formula for heavy and superheavy nuclei with Z{>=}90 and N{>=}140

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dong Tiekuang; Joint Center for Particle, Nuclear Physics and Cosmology, Nanjing 210008; Ren Zhongzhou

2008-06-15

A local formula of binding energy for heavy and superheavy nuclei has very recently been proposed [Dong and Ren, Phys. Rev. C 72, 064331 (2005)]. In this paper, the limit of the predictive ability of this local formula is investigated. It is found that the neutron-proton correlations should be considered when higher precision is required. On the one hand, we introduce a new term |N-Z-50|/A, and on the other hand we consider the different strengths of proton-proton, neutron-neutron, and neutron-proton pairing correlations. For the first time, the standard deviation {radical}({sigma}{sup 2}) of the binding energies for 117 nuclei with Z{>=}90more » and N{>=}140 is reduced to 0.105 MeV. The {alpha} decay energies Q{sub {alpha}} and half-lives T{sub {alpha}} of nuclei with Z=102-118 are reproduced quite well. The proton drip line of superheavy elements from Md (Z=101) to Ds (Z=110) are predicted.« less

Assessment of injection safety in Ha Dong General Hospital, Hanoi, in 2012

PubMed Central

Van Tuong, Phan; Phuong, Tran Thi Minh; Anh, Bui Thi My; Nguyen, Trang Huyen Thi

2017-01-01

Background: Injection is one of the most frequently used medical methods to introduce drugs or other substances into the body for purposes of treatment or prevention. Unsafe injection can cause adverse outcomes, such as abscess and anaphylactic shock, and increases the risk of blood-borne transmission of viruses to patients and health care workers, as well as the community. Recognizing the importance of injection safety, in 2000 the Vietnamese Ministry of Health (MOH) collaborated with the Vietnam Nurses Association to launch the “Safe injection” program throughout the country, including Hanoi. Methods: This cross-sectional study, combining quantitative and qualitative analysis, was conducted from February to August 2012 in Ha Dong General Hospital using a structured questionnaire and observation checklist. The target population of the study was 109 nurses working in clinical departments and 436 injections were observed. Results: The percentage of nurses who are familiar with injection safety standards was found to be 82.6%. The proportion of practical injections that met the 23 standards of injection safety set by the MOH amounted to 22.2%. The factors related to safe injection practice of nurses who were younger age group (OR=3.1; p<0.05) and fewer number of years working as a nurse (OR=2.8; p<0.05). Conclusions: While nurses have high level of knowledge about safe injections but a small proportion actually practiced. Experience may not always guarantee safe practices.  Injection safety training should be regularly imparted upon all categories of nurses. PMID:29188014

Evaluating Physical and Perceptual Responses to Exergames in Chinese Children

PubMed Central

Lau, Patrick W. C.; Liang, Yan; Lau, Erica Y.; Choi, Choung-Rak; Kim, Chang-Gyun; Shin, Myung-Soo

2015-01-01

Purpose: The primary objective of this study was to examine whether exergames could help children reach the recommendations for PA and cardiorespiratory fitness regarding exercise intensity. Differences in perceived physical exertion, EE, VO2, and HR between normal weight (NW) and overweight (OW) children participating in exergames were also examined. Methods: Twenty-one children (age: 10.45 ± 0.88) were assessed for EE, VO2 and HR during rest, in a maximal treadmill test, and while playing different exergames. Ratings of perceived exertion (RPE) (category range: 0 to 10) were also measured during exergaming. Three types of exergames were examined: running, table tennis, and dancing. These games were either performed on a Chinese game console, I-Dong, or another well-developed Western game console (Sony PlayStation 3 or Nintendo Wii). Results: Exergaming resulted in EE (kcal/min) from 2.05–5.14, VO2 (mL/kg/min) from 9.98–25.54, and HR (beats per minute) from 98.05–149.66. Children reported RPE ranging from 1.29 to 5.29. The Chinese exergame, I-Dong Running, was the only game in which children reached a moderate intensity and met the recommended minimum VO2reserve (50%) for cardiorespiratory fitness. Conclusion: Exergames could provide alternative opportunities to enhance children’s physical activity. They could be used as light-to-moderate PA, and with exergames, children can even reach the recommended intensity for developing and maintaining cardiorespiratory fitness. PMID:25872015

Assessment of injection safety in Ha Dong General Hospital, Hanoi, in 2012.

PubMed

Van Tuong, Phan; Phuong, Tran Thi Minh; Anh, Bui Thi My; Nguyen, Trang Huyen Thi

2017-01-01

Background : Injection is one of the most frequently used medical methods to introduce drugs or other substances into the body for purposes of treatment or prevention. Unsafe injection can cause adverse outcomes, such as abscess and anaphylactic shock, and increases the risk of blood-borne transmission of viruses to patients and health care workers, as well as the community. Recognizing the importance of injection safety, in 2000 the Vietnamese Ministry of Health (MOH) collaborated with the Vietnam Nurses Association to launch the "Safe injection" program throughout the country, including Hanoi. Methods : This cross-sectional study, combining quantitative and qualitative analysis, was conducted from February to August 2012 in Ha Dong General Hospital using a structured questionnaire and observation checklist. The target population of the study was 109 nurses working in clinical departments and 436 injections were observed. Results : The percentage of nurses who are familiar with injection safety standards was found to be 82.6%. The proportion of practical injections that met the 23 standards of injection safety set by the MOH amounted to 22.2%. The factors related to safe injection practice of nurses who were younger age group (OR=3.1; p<0.05) and fewer number of years working as a nurse (OR=2.8; p<0.05). Conclusions : While nurses have high level of knowledge about safe injections but a small proportion actually practiced. Experience may not always guarantee safe practices.  Injection safety training should be regularly imparted upon all categories of nurses.

Situation of the Kinh poor and minority women and their use of the Maternal Care and Family Planning Service in Nam Dong Mountainous District, Thuathien-Hue Province, Vietnam.

PubMed

Vo Van, T; Hoat, L N; Jan van Schie, T

2004-01-01

This study aimed to determine the barriers to the use of maternal care and family planning (MCFP) services by the disadvantaged Kinh people and Katu ethnic minority people in the remote and mountainous area of Nam Dong District in Central Vietnam. A survey was conducted using a random sample of 420 mothers with at least one child under the age of 5 years. These data were supplemented by interviews with key informants, focus group discussions and observations. Many barriers were identified. The difficulty of the terrain made travel to healthcare centers difficult. The cost of treatment was a barrier for the poorest people. The quality of the services and facilities, as well as the management of these services was perceived to be unsatisfactory. Traditional practices were often described as being contrary to the doctor's advice, and were presented as the reason for unsafe and unassisted home deliveries. Communication was difficult because of the minority languages of the client groups, the prevalence of illiteracy, and the absence of mass communication in this region. Finally, consulting a male healthcare worker was reported to bring the women shame. There is an urgent need for the MCFP services to build both clinical capacity and health promotion activities in a way that is gender sensitive, cognisant of traditional practices and accessible by both illiterate and minority language speaking people.

Two new species of shovel-jaw carp Onychostoma (Teleostei: Cyprinidae) from southern Vietnam.

PubMed

Hoang, Huy Duc; Pham, Hung Manh; Tran, Ngan Trong

2015-05-22

Two new species of large shovel-jaw carps in the genus Onychostoma are described from the upper Krong No and middle Dong Nai drainages of the Langbiang Plateau in southern Vietnam. These new species are known from streams in montane mixed pine and evergreen forests between 140 and 1112 m. Their populations are isolated in the headwaters of the upper Sre Pok River of the Mekong basin and in the middle of the Dong Nai basin. Both species are differentiated from their congeners by a combination of the following characters: transverse mouth opening width greater than head width, 14-17 predorsal scales, caudal-peduncle length 3.9-4.2 times in SL, no barbels in adults and juveniles, a strong serrated last simple ray of the dorsal fin, and small eye diameter (20.3-21.5% HL). Onychostoma krongnoensis sp. nov. is differentiated from Onychostoma dongnaiensis sp. nov. by body depth (4.0 vs. 3.2 times in SL), predorsal scale number (14-17 vs. 14-15), dorsal-fin length (4.5 vs. 4.2 times in SL), caudal-peduncle length (3.9 vs. 4.2 times in SL), colour in life (dark vs. bright), and by mitochondrial DNA (0.2% sequence divergence). Molecular evidence indicates that both species are members of Onychostoma and are distinct from all congeners sampled (uncorrected sequence divergences at the 16S rRNA gene of >2.0% for all Onychostoma for which homologous 16S rRNA sequences are available).

Wave propagation, scattering and emission in complex media

NASA Astrophysics Data System (ADS)

Jin, Ya-Qiu

I. Polarimetric scattering and SAR imagery. EM wave propagation and scattering in polarimetric SAR interferometry / S. R. Cloude. Terrain topographic inversion from single-pass polarimetric SAR image data by using polarimetric stokes parameters and morphological algorithm / Y. Q. Jin, L. Luo. Road detection in forested area using polarimetric SAR / G. W. Dong ... [et al.]. Research on some problems about SAR radiometric resolution / G. Dong ... [et al.]. A fast image matching algorithm for remote sensing applications / Z. Q. Hou ... [et al.]. A new algorithm of noised remote sensing image fusion based on steerable filters / X. Kang ... [et al.]. Adaptive noise reduction of InSAR data based on anisotropic diffusion models and their applications to phase unwrapping / C. Wang, X. Gao, H. Zhang -- II. Scattering from randomly rough surfaces. Modeling tools for backscattering from rough surfaces / A. K. Fung, K. S. Chen. Pseudo-nondiffracting beams from rough surface scattering / E. R. Méndez, T. A. Leskova, A. A. Maradudin. Surface roughness clutter effects in GPR modeling and detection / C. Rappaport. Scattering from rough surfaces with small slopes / M. Saillard, G. Soriano. Polarization and spectral characteristics of radar signals reflected by sea-surface / V. A. Butko, V. A. Khlusov, L. I. Sharygina. Simulation of microwave scattering from wind-driven ocean surfaces / M. Y. Xia ... [et al.]. HF surface wave radar tests at the Eastern China Sea / X. B. Wu ... [et al.] -- III. Electromagnetics of complex materials. Wave propagation in plane-parallel metamaterial and constitutive relations / A. Ishimaru ... [et al.]. Two dimensional periodic approach for the study of left-handed metamaterials / T. M. Grzegorczyk ... [et al.]. Numerical analysis of the effective constitutive parameters of a random medium containing small chiral spheres / Y. Nanbu, T. Matsuoka, M. Tateiba. Wave propagation in inhomogeneous media: from the Helmholtz to the Ginzburg -Landau equation / M. Gitterman. Transformation of the spectrum of scattered radiation in randomly inhomogeneous absorptive plasma layer / G. V. Jandieri, G. D. Aburjunia, V. G. Jandieri. Numerical analysis of microwave heating on saponification reaction / K. Huang, K. Jia -- IV. Scattering from complex targets. Analysis of electromagnetic scattering from layered crossed-gratings of circular cylinders using lattice sums technique / K. Yasumoto, H. T. Jia. Scattering by a body in a random medium / M. Tateiba, Z. Q. Meng, H. El-Ocla. A rigorous analysis of electromagnetic scattering from multilayered crossed-arrays of metallic cylinders / H. T. Jia, K. Yasumoto. Vector models of non-stable and spatially-distributed radar objects / A. Surkov ... [et al.]. Simulation of algorithm of orthogonal signals forming and processing used to estimate back scattering matrix of non-stable radar objects / D. Nosov ... [et al.]. New features of scattering from a dielectric film on a reflecting metal substrate / Z. H. Gu, I. M. Fuks, M. Ciftan. A higher order FDTD method for EM wave propagation in collision plasmas / S. B. Liu, J. J. Mo, N. C. Yuan -- V. Radiative transfer and remote sensing. Simulating microwave emission from Antarctica ice sheet with a coherent model / M. Tedesco, P. Pampaloni. Scattering and emission from inhomogeneous vegetation canopy and alien target by using three-dimensional Vector Radiative Transfer (3D-VRT) equation / Y. Q. Jin, Z. C. Liang. Analysis of land types using high-resolution satellite images and fractal approach / H. G. Zhang ... [et al.]. Data fusion of RADARSAT SAR and DMSP SSM/I for monitoring sea ice of China's Bohai Sea / Y. Q. Jin. Retrieving atmospheric temperature profiles from simulated microwave radiometer data with artificial neural networks / Z. G. Yao, H. B. Chen -- VI. Wave propagation and wireless communication. Wireless propagation in urban environments: modeling and experimental verification / D. Erricolo ... [et al.]. An overview of physics-based wave propagation in forested environment / K. Sarabandi, I. Koh. Angle-of-arrival fluctuations due to meteorological conditions in the diffraction zone of C-band radio waves, propagated over the ground surface / T. A. Tyufilina, A. A. Meschelyakov, M. V. Krutikov. Simulating radio channel statistics using ray based prediction codes / H. L. Bertoni. Measurement and simulation of ultra wideband antenna elements / W. Sörgel, W. Wiesbeck. The experimental investigation of a ground-placed radio complex synchronization system / V. P. Denisov ... [et al.] -- VII. Computational electromagnetics. Analysis of 3-D electromagnetic wave scattering with the Krylov subspace FFT iterative methods / R. S. Chen ... [et al.]. Sparse approximate inverse preconditioned iterative algorithm with block toeplitz matrix for fast analysis of microstrip circuits / L. Mo, R. S. Chen, E. K. N. Yung. An Efficient modified interpolation technique for the translation operators in MLFMA / J. Hu, Z. P. Nie, G. X. Zou. Efficient solution of 3-D vector electromagnetic scattering by CG-MLFMA with partly approximate iteration / J. Hu, Z. P. Nie. The effective constitution at interface of different media / L. G. Zheng, W. X. Zhang. Novel basis functions for quadratic hexahedral edge element / P. Liu ... [et al.]. A higher order FDTD method for EM wave propagation in collision plasmas / S. B. Liu, J. J. Mo, N. C. Yuan. Attenuation of electric field eradiated by underground source / J. P. Dong, Y. G. Gao.

Inactivation gating of Kv7.1 channels does not involve concerted cooperative subunit interactions.

PubMed

Meisel, Eshcar; Tobelaim, William; Dvir, Meidan; Haitin, Yoni; Peretz, Asher; Attali, Bernard

2018-01-01

Inactivation is an intrinsic property of numerous voltage-gated K + (Kv) channels and can occur by N-type or/and C-type mechanisms. N-type inactivation is a fast, voltage independent process, coupled to activation, with each inactivation particle of a tetrameric channel acting independently. In N-type inactivation, a single inactivation particle is necessary and sufficient to occlude the pore. C-type inactivation is a slower process, involving the outermost region of the pore and is mediated by a concerted, highly cooperative interaction between all four subunits. Inactivation of Kv7.1 channels does not exhibit the hallmarks of N- and C-type inactivation. Inactivation of WT Kv7.1 channels can be revealed by hooked tail currents that reflects the recovery from a fast and voltage-independent inactivation process. However, several Kv7.1 mutants such as the pore mutant L273F generate an additional voltage-dependent slow inactivation. The subunit interactions during this slow inactivation gating remain unexplored. The goal of the present study was to study the nature of subunit interactions along Kv7.1 inactivation gating, using concatenated tetrameric Kv7.1 channel and introducing sequentially into each of the four subunits the slow inactivating pore mutation L273F. Incorporating an incremental number of inactivating mutant subunits did not affect the inactivation kinetics but slowed down the recovery kinetics from inactivation. Results indicate that Kv7.1 inactivation gating is not compatible with a concerted cooperative process. Instead, adding an inactivating subunit L273F into the Kv7.1 tetramer incrementally stabilizes the inactivated state, which suggests that like for activation gating, Kv7.1 slow inactivation gating is not a concerted process.

Wertheim Germany AAF. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F

DTIC Science & Technology

1972-02-17

0600*080C cUS$ Enets (45 s SPEED I 3.0 4- MEAN (KNTS) 1 ! 137 1 1316 1 7.21 222 28.33 38.5 AO% IN OIL I -- SPEI N L]sA......9nE".,9 __ I __I __ __ i2...26#71 286.1 33901 ’.Sol 346 37 2! 35761 137 Zi 37.8 7,2 37,2 35762 38.2i 2: 700 1 92 2270 267 8133_6 I>70 i25127,2[ 288 3. Sat~:i 3626 37:7 38.71 28...43.7180 i 3~ -32 43.p 43. 43,2 -r54.7 713 43,74.7! 43-2, 3 71.7 ____ __43.4 43. 43 43.43 4342 434 43 412 3~7 43437 6 3 j. 43.71 4?.7 1400-3 43 1 C 43,9

Synthesis and Useful Reactions of Organosilicon Polymeric Precursors for Ceramics

DTIC Science & Technology

1992-04-05

composites are hot pressing, chemical vapor infiltration , reaction bonding and polymer infiltration / pyrolysis . Thus the inorganic or organometallic...to prepare preceramic polymers whose D; pyrolysis gives -99% SiC , -99.5% Si 3 N4 , or any mixture of the two by appropriate manipulation of the...the standard furnace pyrolysis of the polymer gave a ceramic of composition 96.6% SiC , 1.7% ZrC and 1.7% Si in 71% yield. Finally, (71-C

37 CFR 251.71 - Commencement of proceedings.

Code of Federal Regulations, 2011 CFR

2011-07-01

.... 251.71 Section 251.71 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS... year in accordance with the requirements of this subchapter. (c) Digital audio recording devices and... manufacture and distribution in the United States, of any digital recording device or medium, any person...

37 CFR 251.71 - Commencement of proceedings.

Code of Federal Regulations, 2010 CFR

2010-07-01

.... 251.71 Section 251.71 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS... year in accordance with the requirements of this subchapter. (c) Digital audio recording devices and... manufacture and distribution in the United States, of any digital recording device or medium, any person...

10 CFR 71.133 - Corrective action.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 2 2011-01-01 2011-01-01 false Corrective action. 71.133 Section 71.133 Energy NUCLEAR....133 Corrective action. The licensee, certificate holder, and applicant for a CoC shall establish... determined and corrective action taken to preclude repetition. The identification of the significant...

10 CFR 71.133 - Corrective action.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Corrective action. 71.133 Section 71.133 Energy NUCLEAR....133 Corrective action. The licensee, certificate holder, and applicant for a CoC shall establish... determined and corrective action taken to preclude repetition. The identification of the significant...

COPI Is Required for Enterovirus 71 Replication

PubMed Central

Wang, Jianmin; Wu, Zhiqiang; Jin, Qi

2012-01-01

Enterovirus 71 (EV71), a member of the Picornaviridae family, is found in Asian countries where it causes a wide range of human diseases. No effective therapy is available for the treatment of these infections. Picornaviruses undergo RNA replication in association with membranes of infected cells. COPI and COPII have been shown to be involved in the formation of picornavirus-induced vesicles. Replication of several picornaviruses, including poliovirus and Echovirus 11 (EV11), is dependent on COPI or COPII. Here, we report that COPI, but not COPII, is required for EV71 replication. Replication of EV71 was inhibited by brefeldin A and golgicide A, inhibitors of COPI activity. Furthermore, we found EV71 2C protein interacted with COPI subunits by co-immunoprecipitation and GST pull-down assay, indicating that COPI coatomer might be directed to the viral replication complex through viral 2C protein. Additionally, because the pathway is conserved among different species of enteroviruses, it may represent a novel target for antiviral therapies. PMID:22662263

Time series analysis of reported cases of hand, foot, and mouth disease from 2010 to 2013 in Wuhan, China.

PubMed

Chen, Banghua; Sumi, Ayako; Toyoda, Shin'ichi; Hu, Quan; Zhou, Dunjin; Mise, Keiji; Zhao, Junchan; Kobayashi, Nobumichi

2015-11-03

Hand, foot, and mouth disease (HFMD) is an infectious disease caused by a group of enteroviruses, including Coxsackievirus A16 (CVA16) and Enterovirus A71 (EV-A71). In recent decades, Asian countries have experienced frequent and widespread HFMD outbreaks, with deaths predominantly among children. In several Asian countries, epidemics usually peak in the late spring/early summer, with a second small peak in late autumn/early winter. We investigated the possible underlying association between the seasonality of HFMD epidemics and meteorological variables, which could improve our ability to predict HFMD epidemics. We used a time series analysis composed of a spectral analysis based on the maximum entropy method (MEM) in the frequency domain and the nonlinear least squares method in the time domain. The time series analysis was applied to three kinds of monthly time series data collected in Wuhan, China, where high-quality surveillance data for HFMD have been collected: (i) reported cases of HFMD, (ii) reported cases of EV-A71 and CVA16 detected in HFMD patients, and (iii) meteorological variables. In the power spectral densities for HFMD and EV-A71, the dominant spectral lines were observed at frequency positions corresponding to 1-year and 6-month cycles. The optimum least squares fitting (LSF) curves calculated for the 1-year and 6-month cycles reproduced the bimodal cycles that were clearly observed in the HFMD and EV-A71 data. The peak months on the LSF curves for the HFMD data were consistent with those for the EV-A71 data. The risk of infection was relatively high at 10 °C ≤ t < 15 °C (t, temperature [°C]) and 15 °C ≤ t < 20 °C, and peaked at 20 °C ≤ t < 25 °C. In this study, the HFMD infections occurring in Wuhan showed two seasonal peaks, in summer (June) and winter (November or December). The results obtained with a time series analysis suggest that the bimodal seasonal peaks in HFMD epidemics are attributable to EV-A71 epidemics. Our results suggest that controlling the spread of EV-A71 infections when the temperature is approximately 20-25 °C should be considered to prevent HFMD infections in Wuhan, China.

Developmental competence of bovine embryos from heat-stressed ova.

PubMed

Edwards, J L; Bogart, A N; Rispoli, L A; Saxton, A M; Schrick, F N

2009-02-01

Because multiple ovulation embryo transfer procedures are occasionally performed in cows experiencing heat stress, the goal of this study was to assess the developmental competence of otherwise morphologically normal embryos from heat-stressed ova. To this end, the ability of compact morulae from heat-stressed and non-heat-stressed bovine ova to undergo blastocyst development after culture at 38.5 or 41.0 degrees C was examined. It was hypothesized that heat-induced perturbations in the ooplasm carry over to increase the susceptibility of the preattachment embryo to heat stress. Initially, ova were matured at 38.5 or 41.0 degrees C. The consequences of heat stress did not include altered cleavage, but did reduce the proportion of 8- to 16-cell-stage embryos (55.3 vs. 50.6%; SEM +/- 1.9). Although proportionately fewer, compact morulae from heat-stressed ova were equivalent in quality to those from non-heat-stressed ova (2.1 and 2.1; SEM = 0.04). Culture of compact morulae from non-heat-stressed ova at 41.0 degrees C did not affect blastocyst development (71.9 and 71.5%; SEM = 3.0). Furthermore, the development of compact morulae from heat-stressed ova was similar to that of non-heat-stressed ova after culture at 38.5 degrees C (68.2 vs. 71.9 and 71.5%; SEM = 3.0). However, blastocyst development was reduced when compact morulae from heat-stressed ova were cultured at 41.0 degrees C (62.3 vs. 71.9, 71.5 and 68.2; SEM = 3.1). In summary, reduced compaction rates of heat-stressed ova explained in part why fewer develop to the blastocyst stage after fertilization. The thermolability of the few embryos that develop from otherwise developmentally challenged ova emphasizes the importance of minimizing exposure to stressor(s) during oocyte maturation.

10 CFR 71.113 - Document control.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 2 2013-01-01 2013-01-01 false Document control. 71.113 Section 71.113 Energy NUCLEAR....113 Document control. The licensee, certificate holder, and applicant for a CoC shall establish measures to control the issuance of documents such as instructions, procedures, and drawings, including...

10 CFR 71.113 - Document control.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 2 2014-01-01 2014-01-01 false Document control. 71.113 Section 71.113 Energy NUCLEAR....113 Document control. The licensee, certificate holder, and applicant for a CoC shall establish measures to control the issuance of documents such as instructions, procedures, and drawings, including...

10 CFR 71.113 - Document control.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Document control. 71.113 Section 71.113 Energy NUCLEAR....113 Document control. The licensee, certificate holder, and applicant for a CoC shall establish measures to control the issuance of documents such as instructions, procedures, and drawings, including...

10 CFR 71.87 - Routine determinations.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 2 2012-01-01 2012-01-01 false Routine determinations. 71.87 Section 71.87 Energy NUCLEAR...; (c) Each closure device of the packaging, including any required gasket, is properly installed and secured and free of defects; (d) Any system for containing liquid is adequately sealed and has adequate...

10 CFR 71.87 - Routine determinations.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 2 2011-01-01 2011-01-01 false Routine determinations. 71.87 Section 71.87 Energy NUCLEAR...; (c) Each closure device of the packaging, including any required gasket, is properly installed and secured and free of defects; (d) Any system for containing liquid is adequately sealed and has adequate...

10 CFR 71.87 - Routine determinations.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 2 2014-01-01 2014-01-01 false Routine determinations. 71.87 Section 71.87 Energy NUCLEAR...; (c) Each closure device of the packaging, including any required gasket, is properly installed and secured and free of defects; (d) Any system for containing liquid is adequately sealed and has adequate...

10 CFR 71.87 - Routine determinations.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 2 2013-01-01 2013-01-01 false Routine determinations. 71.87 Section 71.87 Energy NUCLEAR...; (c) Each closure device of the packaging, including any required gasket, is properly installed and secured and free of defects; (d) Any system for containing liquid is adequately sealed and has adequate...

10 CFR 71.87 - Routine determinations.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Routine determinations. 71.87 Section 71.87 Energy NUCLEAR...; (c) Each closure device of the packaging, including any required gasket, is properly installed and secured and free of defects; (d) Any system for containing liquid is adequately sealed and has adequate...

15 CFR 14.71 - Closeout procedures.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Closeout procedures. 14.71 Section 14.71 Commerce and Foreign Trade Office of the Secretary of Commerce UNIFORM ADMINISTRATIVE REQUIREMENTS... recipient shall promptly refund any balances of unobligated funds that the DoC has advanced or paid and that...

10 CFR 71.123 - Test control.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 2 2011-01-01 2011-01-01 false Test control. 71.123 Section 71.123 Energy NUCLEAR....123 Test control. The licensee, certificate holder, and applicant for a CoC shall establish a test... satisfactorily in service is identified and performed in accordance with written test procedures that incorporate...

45 CFR 260.71 - What definitions apply to this subpart?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 2 2013-10-01 2012-10-01 true What definitions apply to this subpart? 260.71 Section 260.71 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE... waived. (c) Control group and experimental group have the meanings specified in the terms and conditions...

45 CFR 260.71 - What definitions apply to this subpart?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 2 2014-10-01 2012-10-01 true What definitions apply to this subpart? 260.71 Section 260.71 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE... waived. (c) Control group and experimental group have the meanings specified in the terms and conditions...

41 CFR 301-71.100 - What is the purpose of the travel authorization process?

Code of Federal Regulations, 2014 CFR

2014-07-01

... the travel authorization process? 301-71.100 Section 301-71.100 Public Contracts and Property... travel authorization process? The purpose is to: (a) Provide the employee information regarding what... travel programs; (c) Provide financial information necessary for budgetary planning; and (d) Identify...

41 CFR 301-71.100 - What is the purpose of the travel authorization process?

Code of Federal Regulations, 2012 CFR

2012-07-01

... the travel authorization process? 301-71.100 Section 301-71.100 Public Contracts and Property... travel authorization process? The purpose is to: (a) Provide the employee information regarding what... travel programs; (c) Provide financial information necessary for budgetary planning; and (d) Identify...

41 CFR 301-71.100 - What is the purpose of the travel authorization process?

Code of Federal Regulations, 2010 CFR

2010-07-01

... the travel authorization process? 301-71.100 Section 301-71.100 Public Contracts and Property... travel authorization process? The purpose is to: (a) Provide the employee information regarding what... travel programs; (c) Provide financial information necessary for budgetary planning; and (d) Identify...

41 CFR 301-71.100 - What is the purpose of the travel authorization process?

Code of Federal Regulations, 2013 CFR

2013-07-01

... the travel authorization process? 301-71.100 Section 301-71.100 Public Contracts and Property... travel authorization process? The purpose is to: (a) Provide the employee information regarding what... travel programs; (c) Provide financial information necessary for budgetary planning; and (d) Identify...

48 CFR 1515.305-71 - Documentation of proposal evaluation.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Ethics ethics provisions at 5 CFR part 2635. (c) Any information which might reveal that an offeror has... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Documentation of proposal evaluation. 1515.305-71 Section 1515.305-71 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION...

Fragment-wise design of inhibitors to 3C proteinase from enterovirus 71.

PubMed

Wu, Caiming; Zhang, Lanjun; Li, Peng; Cai, Qixu; Peng, Xuanjia; Yin, Ke; Chen, Xinsheng; Ren, Haixia; Zhong, Shilin; Weng, Yuwei; Guan, Yi; Chen, Shuhui; Wu, Jinzhun; Li, Jian; Lin, Tianwei

2016-06-01

Enterovirus 71 (EV71) is a causative agent of hand, foot and mouth disease (HFMD), which can spread its infection to central nervous and other systems with severe consequence. A key factor in the replication of EV71 is its 3C proteinase (3C(pro)), a significant drug target. Peptidomimetics were employed as inhibitors of this enzyme for developing antivirals. However, the peptide bonds in these peptidomimetics are a source of low bioavailability due to their susceptibility to protease digestion. To produce non-peptidomimetic inhibitors by replacing these peptide bonds, it would be important to gain better understanding on the contribution of each component to the interaction and potency. A series of compounds of different lengths targeting 3C(pro) and having an α,β-unsaturated ester as the warhead were synthesized and their interactions with the enzyme were evaluated by complex structure analyses and potency assays for a better understanding on the relationship between potency and evolution of interaction. The P2 moiety of the compound would need to be oriented to interact in the S2 site in the substrate binding cleft and the P3-P4 moieties were required to generate sufficient potency. A hydrophobic terminal group will benefit the cellular uptake and improve the activity in vivo. The data presented here provide a basis for designing a new generation of non-peptidomimetics to target EV71 3C(pro). Copyright © 2016 Elsevier B.V. All rights reserved.

An A−71C substitution in a green gene at the second position in the red/green visual-pigment gene array is associated with deutan color-vision deficiency

PubMed Central

Ueyama, Hisao; Li, Yao-Hua; Fu, Gui-Lian; Lertrit, Patcharee; Atchaneeyasakul, La-ongsri; Oda, Sanae; Tanabe, Shoko; Nishida, Yasuhiro; Yamade, Shinichi; Ohkubo, Iwao

2003-01-01

We studied 247 Japanese males with congenital deutan color-vision deficiency and found that 37 subjects (15.0%) had a normal genotype of a single red gene followed by a green gene(s). Two of them had missense mutations in the green gene(s), but the other 35 subjects had no mutations in either the exons or their flanking introns. However, 32 of the 35 subjects, including all 8 subjects with pigment-color defect, a special category of deuteranomaly, had a nucleotide substitution, A−71C, in the promoter of a green gene at the second position in the red/green visual-pigment gene array. Although the −71C substitution was also present in color-normal Japanese males at a frequency of 24.3%, it was never at the second position but always found further downstream. The substitution was found in 19.4% of Chinese males and 7.7% of Thai males but rarely in Caucasians or African Americans. These results suggest that the A−71C substitution in the green gene at the second position is closely associated with deutan color-vision deficiency. In Japanese and presumably other Asian populations further downstream genes with −71C comprise a reservoir of the visual-pigment genes that cause deutan color-vision deficiency by unequal crossing over between the intergenic regions. PMID:12626747

Relationship between HgbA1c and myocardial blood flow reserve in patients with type 2 diabetes mellitus: noninvasive assessment using real-time myocardial perfusion echocardiography.

PubMed

Huang, Runqing; Abdelmoneim, Sahar S; Nhola, Lara F; Mulvagh, Sharon L

2014-01-01

To study the relationship between glycosylated hemoglobin (HgbA1c) and myocardial perfusion in type 2 diabetes mellitus (T2DM) patients, we prospectively enrolled 24 patients with known or suspected coronary artery disease (CAD) who underwent adenosine stress by real-time myocardial perfusion echocardiography (RTMPE). HgbA1c was measured at time of RTMPE. Microbubble velocity (β min(-1)), myocardial blood flow (MBF, mL/min/g), and myocardial blood flow reserve (MBFR) were quantified. Quantitative MCE analysis was feasible in all patients (272/384 segments, 71%). Those with HgbA1c > 7.1% had significantly lower βreserve and MBFR than those with HgbA1c ≤ 7.1% (P < 0.05). In patients with suspected CAD, there was a significant inverse correlation between MBFR and HgbA1c (r = -0.279, P = 0.01); however, in those with known CAD, this relationship was not significant (r = -0.117, P = 0.129). Using a MBFR cutoff value > 2 as normal, HgbA1c > 7.1% significantly increased the risk for abnormal MBFR, (adjusted odds ratio: 1.92, 95% CI: 1.12-3.35, P = 0.02). Optimal glycemic control is associated with preservation of MBFR as determined by RTMPE, in T2DM patients at risk for CAD.

Ultra-high Thermal Conductivity of Spider Silk: Protein Function Study with Controlled Structure Change and Comparison

DTIC Science & Technology

2016-01-23

a) Papers published in peer-reviewed journals (N/A for none) Enter List of papers submitted or published that acknowledge ARO support from the start...of the project to the date of this printing. List the papers , including journal references, in the following categories: 11.00 10.00 20.00 18.00...Received Paper 1.00 4.00 3.00 2.00 5.00 8.00 Huan Lin, Shen Xu, Chong Li, Hua Dong, Xinwei Wang. Thermal and electrical conduction in 6.4 nm thin gold films

A generic assay for whole-genome amplification and deep sequencing of enterovirus A71

PubMed Central

Tan, Le Van; Tuyen, Nguyen Thi Kim; Thanh, Tran Tan; Ngan, Tran Thuy; Van, Hoang Minh Tu; Sabanathan, Saraswathy; Van, Tran Thi My; Thanh, Le Thi My; Nguyet, Lam Anh; Geoghegan, Jemma L.; Ong, Kien Chai; Perera, David; Hang, Vu Thi Ty; Ny, Nguyen Thi Han; Anh, Nguyen To; Ha, Do Quang; Qui, Phan Tu; Viet, Do Chau; Tuan, Ha Manh; Wong, Kum Thong; Holmes, Edward C.; Chau, Nguyen Van Vinh; Thwaites, Guy; van Doorn, H. Rogier

2015-01-01

Enterovirus A71 (EV-A71) has emerged as the most important cause of large outbreaks of severe and sometimes fatal hand, foot and mouth disease (HFMD) across the Asia-Pacific region. EV-A71 outbreaks have been associated with (sub)genogroup switches, sometimes accompanied by recombination events. Understanding EV-A71 population dynamics is therefore essential for understanding this emerging infection, and may provide pivotal information for vaccine development. Despite the public health burden of EV-A71, relatively few EV-A71 complete-genome sequences are available for analysis and from limited geographical localities. The availability of an efficient procedure for whole-genome sequencing would stimulate effort to generate more viral sequence data. Herein, we report for the first time the development of a next-generation sequencing based protocol for whole-genome sequencing of EV-A71 directly from clinical specimens. We were able to sequence viruses of subgenogroup C4 and B5, while RNA from culture materials of diverse EV-A71 subgenogroups belonging to both genogroup B and C was successfully amplified. The nature of intra-host genetic diversity was explored in 22 clinical samples, revealing 107 positions carrying minor variants (ranging from 0 to 15 variants per sample). Our analysis of EV-A71 strains sampled in 2013 showed that they all belonged to subgenogroup B5, representing the first report of this subgenogroup in Vietnam. In conclusion, we have successfully developed a high-throughput next-generation sequencing-based assay for whole-genome sequencing of EV-A71 from clinical samples. PMID:25704598

Cloning and characterization of a novel stress-responsive WRKY transcription factor gene (MusaWRKY71) from Musa spp. cv. Karibale Monthan (ABB group) using transformed banana cells.

PubMed

Shekhawat, Upendra K Singh; Ganapathi, Thumballi R; Srinivas, Lingam

2011-08-01

WRKY transcription factor proteins play significant roles in plant stress responses. Here, we report the cloning and characterization of a novel WRKY gene, MusaWRKY71 isolated from an edible banana cultivar Musa spp. Karibale Monthan (ABB group). MusaWRKY71, initially identified using in silico approaches from an abiotic stress-related EST library, was later extended towards the 3' end using rapid amplification of cDNA ends technique. The 1299-bp long cDNA of MusaWRKY71 encodes a protein with 280 amino acids and contains a characteristic WRKY domain in the C-terminal half. Although MusaWRKY71 shares good similarity with other monocot WRKY proteins the substantial size difference makes it a unique member of the WRKY family in higher plants. The 918-bp long 5' proximal region determined using thermal asymmetric interlaced-polymerase chain reaction has many putative cis-acting elements and transcription factor binding motifs. Subcellular localization assay of MusaWRKY71 performed using a GFP-fusion platform confirmed its nuclear targeting in transformed banana suspension cells. Importantly, MusaWRKY71 expression in banana plantlets was up-regulated manifold by cold, dehydration, salt, ABA, H2O2, ethylene, salicylic acid and methyl jasmonate treatment indicating its involvement in response to a variety of stress conditions in banana. Further, transient overexpression of MusaWRKY71 in transformed banana cells led to the induction of several genes, homologues of which have been proven to be involved in diverse stress responses in other important plants. The present study is the first report on characterization of a banana stress-related transcription factor using transformed banana cells.

Ca2+-Calmodulin and PIP2 interactions at the proximal C-terminus of Kv7 channels.

PubMed

Tobelaim, William S; Dvir, Meidan; Lebel, Guy; Cui, Meng; Buki, Tal; Peretz, Asher; Marom, Milit; Haitin, Yoni; Logothetis, Diomedes E; Hirsch, Joel A; Attali, Bernard

2017-11-02

In the heart, co-assembly of Kv7.1 with KCNE1 produces the slow I KS potassium current, which repolarizes the cardiac action potential and mutations in human Kv7.1 and KCNE1 genes cause cardiac arrhythmias. The proximal Kv7.1 C-terminus binds calmodulin (CaM) and phosphatidylinositol-4,5-bisphosphate (PIP 2 ) and recently we revealed the competition of PIP 2 with the calcified CaM N-lobe to a previously unidentified site in Kv7.1 helix B, also known to harbor a LQT mutation. Data indicated that PIP 2 and Ca 2+ -CaM perform the same function on I KS channel gating to stabilize the channel open state. Here we show that similar features were observed for Kv7.1 currents expressed alone. We also find that conservation of homologous residues in helix B of other Kv7 subtypes confer similar competition of Ca 2+ -CaM with PIP2 binding to their proximal C-termini and suggest that PIP2-CaM interactions converge to Kv7 helix B to modulates channel activity in a Kv7 subtype-dependent manner.

Effect of ambient temperature on the efficiency of the PCPDTBT: PC71BM BHJ solar cells

NASA Astrophysics Data System (ADS)

Ahmad, Zubair; Touati, Farid; Muhammad, Fahmi F.; Najeeb, Mansoor Ani; Shakoor, R. A.

2017-07-01

In this research article, the influence of environment temperature on the performance of the organic bulk heterojunction organic solar cells has been investigated. We describe the effect of ambient temperature on the efficiency of poly-[2,6-(4,4-bis-(2-ethylhexyl)-4H-cyclopenta-[2,1-b;3,4-b']dithiophene)-alt-4,7-(2,1,3-benzothiadiazole)] (PCPDTBT) and [6, 6]-phenylC71-butyric-acid-methyl-ester (PC71BM)-based bulk heterojunction (BHJ) organic solar cells. The current-voltage characteristics of the ITO/PEDOT:PSS/PCPDTBT:PC71BM/Al solar cells are recorded in the temperature range of 25-60 °C under 100 mW/cm2 solar irradiation. The short-circuit current ( J sc) of the solar cells increased from 4.28 to 9.23 mAcm-2 when the temperature elevated from 25 to 55 °C. However, the open-circuit voltage ( V oc) and fill factor (FF) of the cells almost remained unchanged over the whole investigated temperature range. The values of V oc and FF are found to be 0.58 ± 01 and 0.60 ± 0.12 V, respectively. The results clearly indicate that the maximum efficiency of the ITO/PEDOT:PSS/PCPDTBT:PC71BM/Al solar cells can be achieved in the range of 52-58 °C.

78 FR 19164 - Amendments to Compliance Certification Content Requirements for State and Federal Operating...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-29

...-AQ71 Amendments to Compliance Certification Content Requirements for State and Federal Operating... direct final rulemaking for the part 70 program reads as follows: Sec. 70.6 Permit content. * * * * * (c... the 2001 direct final rulemaking for the part 71 program reads as follows: Sec. 71.6 Permit content...

14 CFR 71.33 - Class A airspace areas.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Class A airspace areas. 71.33 Section 71.33 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRSPACE... of the earth and the Alaska Peninsula west of longitude 160°00′00″ West. (c) The airspace areas...

Growth and properties of rare-earth arsenide InGaAs nanocomposites for terahertz generation

NASA Astrophysics Data System (ADS)

Salas, R.; Guchhait, S.; Sifferman, S. D.; McNicholas, K. M.; Dasika, V. D.; Krivoy, E. M.; Jung, D.; Lee, M. L.; Bank, S. R.

2015-02-01

We explore the electrical, optical, and structural properties of fast photoconductors of In0.53Ga0.47As containing a number of different rare-earth arsenide nanostructures. The rare-earth species provides a route to tailor the properties of the photoconductive materials. LuAs, GdAs, and LaAs nanostructures were embedded into InGaAs in a superlattice structure and compared to the relatively well-studied ErAs:InGaAs system. LaAs:InGaAs was found to have the highest dark resistivities, while GdAs:InGaAs had the lowest carrier lifetimes and highest carrier mobility at moderate depositions. The quality of the InGaAs overgrowth appears to have the most significant effect on the properties of these candidate fast photoconductors.

An Orbital Trap Mass Analyzer Using a Hybrid Magnetic-Electric Field: A Simulation Study

NASA Astrophysics Data System (ADS)

Xu, Chongsheng; Wu, Fangling; Ding, Li; Ding, Chuan-Fan

2018-03-01

An orbital ion trap mass analyzer employing hybrid magnetic-electric field was designed and simulated. The trap has a rotational symmetrical structure and the hybrid trapping field was created in a toroidal space between 12 pairs of sector detection electrodes. Ion injection and ion orbital motion inside the trap were simulated using SIMION 8.1 with a user Lua program, and the required electric and magnetic field were investigated. The image charge signal can be picked up by the 12 pairs of detection electrodes and the mass resolution was evaluated using FFT. The simulated resolving power for the optimized configuration over 79,000 FWHM was obtained at the magnetic induction intensity of 0.5 Tesla in the simulation. [Figure not available: see fulltext.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Micheva, Genoveva; Oey, M. S.; Jaskot, Anne E.

We present the remarkable discovery that the dwarf irregular galaxy NGC 2366 is an excellent analog of the Green Pea (GP) galaxies, which are characterized by extremely high ionization parameters. The similarities are driven predominantly by the giant H ii region Markarian 71 (Mrk 71). We compare the system with GPs in terms of morphology, excitation properties, specific star-formation rate, kinematics, absorption of low-ionization species, reddening, and chemical abundance, and find consistencies throughout. Since extreme GPs are associated with both candidate and confirmed Lyman continuum (LyC) emitters, Mrk 71/NGC 2366 is thus also a good candidate for LyC escape. Themore » spatially resolved data for this object show a superbubble blowout generated by mechanical feedback from one of its two super star clusters (SSCs), Knot B, while the extreme ionization properties are driven by the ≲1 Myr-old, enshrouded SSC Knot A, which has ∼10 times higher ionizing luminosity. Very massive stars (>100 M {sub ⊙}) may be present in this remarkable object. Ionization-parameter mapping indicates that the blowout region is optically thin in the LyC, and the general properties also suggest LyC escape in the line of sight. Mrk 71/NGC 2366 does differ from GPs in that it is one to two orders of magnitude less luminous. The presence of this faint GP analog and candidate LyC emitter (LCE) so close to us suggests that LCEs may be numerous and commonplace, and therefore could significantly contribute to the cosmic ionizing budget. Mrk 71/NGC 2366 offers an unprecedentedly detailed look at the viscera of a candidate LCE, and could clarify the mechanisms of LyC escape.« less

Detection of Cryptosporidium parvum and Cyclospora cayetanensis infections among people living in a slum area in Kathmandu valley, Nepal.

PubMed

Bhattachan, Balkrishna; Sherchand, Jeevan Bahadhur; Tandukar, Sarmila; Dhoubhadel, Bhim Gopal; Gauchan, Leesa; Rai, Ganesh

2017-09-07

The aim of this study is to determine the prevalence of Cyclospora cayetanensis and Cryptosporidium parvum infections among people living a slum in Kathmandu valley, Nepal. Ten different parasites were detected in the stool samples; the prevalence of any parasite was in 27.1% (71/262). The prevalence of C. cayetanensis and C. parvum were 14.1% (10/71) and 5.6% (4/71), respectively. This study showed high prevalence of intestinal parasitic infections along with the coccidian parasites in the slum area of Kathmandu Valley.

A homozygous nonsense CEP250 mutation combined with a heterozygous nonsense C2orf71 mutation is associated with atypical Usher syndrome.

PubMed

Khateb, Samer; Zelinger, Lina; Mizrahi-Meissonnier, Liliana; Ayuso, Carmen; Koenekoop, Robert K; Laxer, Uri; Gross, Menachem; Banin, Eyal; Sharon, Dror

2014-07-01

Usher syndrome (USH) is a heterogeneous group of inherited retinitis pigmentosa (RP) and sensorineural hearing loss (SNHL) caused by mutations in at least 12 genes. Our aim is to identify additional USH-related genes. Clinical examination included visual acuity test, funduscopy and electroretinography. Genetic analysis included homozygosity mapping and whole exome sequencing (WES). A combination of homozygosity mapping and WES in a large consanguineous family of Iranian Jewish origin revealed nonsense mutations in two ciliary genes: c.3289C>T (p.Q1097*) in C2orf71 and c.3463C>T (p.R1155*) in centrosome-associated protein CEP250 (C-Nap1). The latter has not been associated with any inherited disease and the c.3463C>T mutation was absent in control chromosomes. Patients who were double homozygotes had SNHL accompanied by early-onset and severe RP, while patients who were homozygous for the CEP250 mutation and carried a single mutant C2orf71 allele had SNHL with mild retinal degeneration. No ciliary structural abnormalities in the respiratory system were evident by electron microscopy analysis. CEP250 expression analysis of the mutant allele revealed the generation of a truncated protein lacking the NEK2-phosphorylation region. A homozygous nonsense CEP250 mutation, in combination with a heterozygous C2orf71 nonsense mutation, causes an atypical form of USH, characterised by early-onset SNHL and a relatively mild RP. The severe retinal involvement in the double homozygotes indicates an additive effect caused by nonsense mutations in genes encoding ciliary proteins. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

ALS-causing profilin-1-mutant forms a non-native helical structure in membrane environments.

PubMed

Lim, Liangzhong; Kang, Jian; Song, Jianxing

2017-11-01

Despite having physiological functions completely different from superoxide dismutase 1 (SOD1), profilin 1 (PFN1) also carries mutations causing amyotrophic lateral sclerosis (ALS) with a striking similarity to that triggered by SOD1 mutants. Very recently, the C71G-PFN1 has been demonstrated to cause ALS by a gain of toxicity and the acceleration of motor neuron degeneration preceded the accumulation of its aggregates. Here by atomic-resolution NMR determination of conformations and dynamics of WT-PFN1 and C71G-PFN1 in aqueous buffers and in membrane mimetics DMPC/DHPC bicelle and DPC micelle, we deciphered that: 1) the thermodynamic destabilization by C71G transforms PFN1 into coexistence with the unfolded state, which is lacking of any stable tertiary/secondary structures as well as restricted ps-ns backbone motions, thus fundamentally indistinguishable from ALS-causing SOD1 mutants. 2) Most strikingly, while WT-PFN1 only weakly interacts with DMPC/DHPC bicelle without altering the native structure, C71G-PFN1 acquires abnormal capacity in strongly interacting with DMPC/DHPC bicelle and DPC micelle, energetically driven by transforming the highly disordered unfolded state into a non-native helical structure, similar to what has been previously observed on ALS-causing SOD1 mutants. Our results imply that one potential mechanism for C71G-PFN1 to initiate ALS might be the abnormal interaction with membranes as recently established for SOD1 mutants. Copyright © 2017 Elsevier B.V. All rights reserved.

Secular trends for age at spermarche among Chinese boys from 11 ethnic minorities, 1995–2010: a multiple cross-sectional study

PubMed Central

Song, Yi; Ma, Jun; Li, Liu-Bai; Dong, Bin; Wang, Zhiqiang; Agardh, Anette

2016-01-01

Objectives We compared the differences in median age at spermarche among 11 ethnic minorities in 2010, estimated the trends regarding age at spermarche in different ethnic minorities from 1995 to 2010, and explored the association of spermarche with body mass index (BMI). Methods We used four cross-sectional Chinese National Surveys on Students’ Constitution and Health (CNSSCH, 1995, 2000, 2005 and 2010), and the total sample size was 40 113 children aged 11–18 years. The median age at spermarche of each ethnic minority was determined by using probit analysis. Logistic regression was used to assess the association of spermarche with BMI. Results In 2010, the ethnic minorities with earliest age at spermarche were Qiang (12.03 years), Zhuang (12.91 years) and Kirghiz (13.17 years); the three ethnic minorities with latest age at spermarche were Dong (14.73 years), Yao (14.60 years), and Naxi (14.36 years). From 1995 to 2010, age at spermarche showed a decline in almost each minority group except Yao and Dong. A higher BMI was associated with an increased likelihood of having reached spermarche after adjusting for age, regions or ethnic minorities. Conclusions A large variation in age at spermarche was observed among different ethnic minorities. The age at spermarche showed a downward shift in almost each of the 11 ethnic minorities with different patterns over time, and the children with higher BMI are more likely to enter puberty early. PMID:26911588

48 CFR 215.404-71-4 - Facilities capital employed.

Code of Federal Regulations, 2013 CFR

2013-10-01

... the facilities capital cost of money and capital employed using— (1) An analysis of the appropriate Forms CASB-CMF and cost of money factors (48 CFR 9904.414 and FAR 31.205-10); and (2) DD Form 1861, Contract Facilities Capital Cost of Money. (c) Use of DD Form 1861. See PGI 215.404-71-4(c) for obtaining...

48 CFR 215.404-71-4 - Facilities capital employed.

Code of Federal Regulations, 2012 CFR

2012-10-01

... the facilities capital cost of money and capital employed using— (1) An analysis of the appropriate Forms CASB-CMF and cost of money factors (48 CFR 9904.414 and FAR 31.205-10); and (2) DD Form 1861, Contract Facilities Capital Cost of Money. (c) Use of DD Form 1861. See PGI 215.404-71-4(c) for obtaining...

48 CFR 215.404-71-4 - Facilities capital employed.

Code of Federal Regulations, 2014 CFR

2014-10-01

... the facilities capital cost of money and capital employed using— (1) An analysis of the appropriate Forms CASB-CMF and cost of money factors (48 CFR 9904.414 and FAR 31.205-10); and (2) DD Form 1861, Contract Facilities Capital Cost of Money. (c) Use of DD Form 1861. See PGI 215.404-71-4(c) for obtaining...

11 CFR 300.71 - Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)).

Code of Federal Regulations, 2010 CFR

2010-01-01

....71 Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)). No individual described in 11 CFR 300.70 shall spend any funds for a public communication that refers to a clearly... limitations, prohibitions, and reporting requirements of the Act. See definition of public communication at 11...

11 CFR 300.71 - Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)).

Code of Federal Regulations, 2011 CFR

2011-01-01

....71 Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)). No individual described in 11 CFR 300.70 shall spend any funds for a public communication that refers to a clearly... limitations, prohibitions, and reporting requirements of the Act. See definition of public communication at 11...

11 CFR 300.71 - Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)).

Code of Federal Regulations, 2014 CFR

2014-01-01

....71 Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)). No individual described in 11 CFR 300.70 shall spend any funds for a public communication that refers to a clearly... limitations, prohibitions, and reporting requirements of the Act. See definition of public communication at 11...

11 CFR 300.71 - Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)).

Code of Federal Regulations, 2013 CFR

2013-01-01

....71 Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)). No individual described in 11 CFR 300.70 shall spend any funds for a public communication that refers to a clearly... limitations, prohibitions, and reporting requirements of the Act. See definition of public communication at 11...

11 CFR 300.71 - Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)).

Code of Federal Regulations, 2012 CFR

2012-01-01

....71 Federal funds required for certain public communications (2 U.S.C. 441i(f)(1)). No individual described in 11 CFR 300.70 shall spend any funds for a public communication that refers to a clearly... limitations, prohibitions, and reporting requirements of the Act. See definition of public communication at 11...

Genetic Diversity of Enterovirus A71, India

PubMed Central

Saxena, Vinay K.; Sane, Sudhir; Nadkarni, Sushma S.; Sharma, Deepa K.

2015-01-01

We have identified circulation of 3 genogroups of enterovirus (EV) A71 in India. A new genogroup (proposed designation G) was discovered during this study. We isolated genogroups D and G in wide geographic areas but detected subgenogroup C1 only in 1 focus in western India. A systematic nationwide search for EV-A71 is warranted. PMID:25531549

Design Guide for Bolted Joints in Composite Structures

DTIC Science & Technology

1986-03-01

I PR N E 0 0.AND. BI’ 11C. 1 .0) tBP22 00005800 IF(IIBP EO.1) 11LIM2%1 00005810 ^O 71 LOtol1.NLIM2 00005820 DO 22 IL0I.115P 00005350 LiC 20...00006970 IF(JNT.EQ.0) G0 TO 410 -- 00006980 IF~tJLIM2.EQ.1) 00 10 90 00006990 IF04TFL.EQ.0.ANO.’:L1M2.GT.1) 00 TO 90 00007000 71 CONTINUE 00007010 410...00020910 Do 70 I:NI,NZ 00020920 JaI-2 00020930 e.IFCT.EO.N1) 00 TO 71 00020950 145 00 TO 7200020970Ii1: -XC.~~-.A2NLKNLC)2 00020980 7 -2, A MA(I 2)6.1C2

miR-7-1 POTENTIATED ESTROGEN RECEPTOR AGONISTS FOR FUNCTIONAL NEUROPROTECTION IN VSC4.1 MOTONEURONS

PubMed Central

CHAKRABARTI, M.; BANIK, N. L.; RAY, S. K.

2013-01-01

Protection of motoneurons is an important goal in the treatment of spinal cord injury (SCI). We tested whether neuroprotective microRNAs (miRs) like miR-206, miR-17, miR-21, miR-7-1, and miR-106a could enhance efficacy of estrogen receptor (ER) agonists such as 1,3,5-tris (4-hydroxyphenyl)-4-propyl-1H-pyrazole (PPT, ERα agonist), Way200070 (WAY, ERβ agonist), and estrogen (EST, ERα and ERβ agonist) in preventing apoptosis in the calcium ionophore (CI) insulted VSC4.1 motoneurons. We determined that 200 nM CI induced 70% cell death. Treatment with 50 nM PPT, 100 nM WAY, and 150 nM EST induced overexpression of ERα, ERβ, and both receptors, respectively, at mRNA and protein levels. Treatment with ER agonists significantly upregulated miR-206, miR-17, and miR-7-1 in the CI insulted VSC4.1 motoneurons. Transfection with miR-206, miR-17, or miR-7-1 mimic potentiated WAY or EST to inhibit apoptosis in the CI insulted VSC4.1 motoneurons. Overexpression of miR-7-1 maximally increased efficacy of WAY and EST for down regulation of pro-apoptotic Bax and upregulation of anti-apoptotic Bcl-2. A search using miRDB indicated that miR-7-1 could inhibit expression of L-type Ca2+ channel protein alpha 1C (CPα1C). miR-7-1 overexpression and WAY or EST treatment down regulated CPα1C but upregulated p-Akt to trigger cell survival signaling. The same therapeutic strategy increased expression of the Ca2+/calmodulin-dependent protein kinase II beta (CaMKIIβ) and the phosphorylated cAMP response element binding protein (p-CREB) so as to promote Bcl-2 transcription. Whole cell membrane potential and mitochondrial membrane potential studies indicated that miR-7-1 highly potentiated EST to preserve functionality in the CI insulted VSC4.1 motoneurons. In conclusion, our data indicated that miR-7-1 most significantly potentiated efficacy of EST for functional neuroprotection and this therapeutic strategy could be used in the future to attenuate apoptosis of motoneurons in SCI. PMID:24157932

MiR-7-1 potentiated estrogen receptor agonists for functional neuroprotection in VSC4.1 motoneurons.

PubMed

Chakrabarti, M; Banik, N L; Ray, S K

2014-01-03

Protection of motoneurons is an important goal in the treatment of spinal cord injury (SCI). We tested whether neuroprotective microRNAs (miRs) like miR-206, miR-17, miR-21, miR-7-1, and miR-106a could enhance efficacy of estrogen receptor (ER) agonists such as 1,3,5-tris (4-hydroxyphenyl)-4-propyl-1H-pyrazole (PPT, ERα agonist), Way200070 (WAY, ERβ agonist), and estrogen (EST, ERα and ERβ agonist) in preventing apoptosis in the calcium ionophore (CI)-insulted ventral spinal cord 4.1 (VSC4.1) motoneurons. We determined that 200 nM CI induced 70% cell death. Treatment with 50 nM PPT, 100 nM WAY, and 150 nM EST induced overexpression of ERα, ERβ, and both receptors, respectively, at mRNA and protein levels. Treatment with ER agonists significantly upregulated miR-206, miR-17, and miR-7-1 in the CI-insulted VSC4.1 motoneurons. Transfection with miR-206, miR-17, or miR-7-1 mimic potentiated WAY or EST to inhibit apoptosis in the CI-insulted VSC4.1 motoneurons. Overexpression of miR-7-1 maximally increased efficacy of WAY and EST for down regulation of pro-apoptotic Bax and upregulation of anti-apoptotic Bcl-2. A search using microRNA database (miRDB) indicated that miR-7-1 could inhibit the expression of L-type Ca(2+) channel protein alpha 1C (CPα1C). miR-7-1 overexpression and WAY or EST treatment down regulated CPα1C but upregulated p-Akt to trigger cell survival signaling. The same therapeutic strategy increased expression of the Ca(2+)/calmodulin-dependent protein kinase II beta (CaMKIIβ) and the phosphorylated cAMP response element binding protein (p-CREB) so as to promote Bcl-2 transcription. Whole cell membrane potential and mitochondrial membrane potential studies indicated that miR-7-1 highly potentiated EST to preserve functionality in the CI-insulted VSC4.1 motoneurons. In conclusion, our data indicated that miR-7-1 most significantly potentiated efficacy of EST for functional neuroprotection and this therapeutic strategy could be used in the future to attenuate apoptosis of motoneurons in SCI. Copyright © 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

AGEs and HMGB1 Increase Inflammatory Cytokine Production from Human Placental Cells, Resulting in an Enhancement of Monocyte Migration.

PubMed

Shirasuna, Koumei; Seno, Kotomi; Ohtsu, Ayaka; Shiratsuki, Shogo; Ohkuchi, Akihide; Suzuki, Hirotada; Matsubara, Shigeki; Nagayama, Shiho; Iwata, Hisataka; Kuwayama, Takehito

2016-05-01

Advanced glycation end products (AGEs) and high-mobility group box-1 (HMGB1) are considered contributing to placental inflammation. We examined the effect of AGEs and HMGB1 on cytokines from Sw.71 human trophoblast cell lines and the interactions between Sw.71 cells and THP-1-monocytes. Sw.71 cells were cultured with/without AGEs or HMGB1. We examined the role of AGEs or HMGB1 on THP1 migration and effect of AGEs on IL-6 from Sw.71 cells using co-cultures or conditioned medium from THP-1 cells. AGEs and HMGB1 increased interleukin (IL)-6, IL-8, and chemokine C-C motif ligand 2 (CCL2) secretion from Sw.71 cells. The secretion of IL-6 was dependent on reactive oxygen species (ROS) and NF-κB. AGEs stimulated IL-6 secretion through receptor RAGE and TLR4, whereas HMGB1 stimulated it through TLR4. AGEs, but not HMGB1, increased monocyte migration via IL-8 and CCL2 from Sw.71 cells. THP-1 monocytes induced IL-6 secretion from Sw.71 cells, and AGEs further stimulated it. AGEs and HMGB1 may promote sterile placental inflammation cooperating with monocytes/macrophages. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Nonlethal sec71-1 and sec72-1 mutations eliminate proteins associated with the Sec63p-BiP complex from S. cerevisiae.

PubMed Central

Fang, H; Green, N

1994-01-01

The sec71-1 and sec72-1 mutations were identified by a genetic assay that monitored membrane protein integration into the endoplasmic reticulum (ER) membrane of the yeast Saccharomyces cerevisiae. The mutations inhibited integration of various chimeric membrane proteins and translocation of a subset of water soluble proteins. In this paper we show that SEC71 encodes the 31.5-kDa transmembrane glycoprotein (p31.5) and SEC72 encodes the 23-kDa protein (p23) of the Sec63p-BiP complex. SEC71 is therefore identical to SEC66 (HSS1), which was previously shown to encode p31.5. DNA sequence analyses reveal that sec71-1 cells contain a nonsense mutation that removes approximately two-thirds of the cytoplasmic C-terminal domain of p31.5. The sec72-1 mutation shifts the reading frame of the gene encoding p23. Unexpectedly, the sec71-1 mutant lacks p31.5 and p23. Neither mutation is lethal, although sec71-1 cells exhibit a growth defect at 37 degrees C. These results show that p31.5 and p23 are important for the trafficking of a subset of proteins to the ER membrane. Images PMID:7841522

15 CFR 14.71 - Closeout procedures.

Code of Federal Regulations, 2010 CFR

2010-01-01

... COMMERCIAL ORGANIZATIONS After-the-Award Requirements § 14.71 Closeout procedures. (a) Recipients shall... recipient shall promptly refund any balances of unobligated funds that the DoC has advanced or paid and that...

Co-circulation of multiple subtypes of enterovirus A71 (EV- A71) genotype C, including novel recombinants characterised by use of whole genome sequencing (WGS), Denmark 2016.

PubMed

Midgley, Sofie E; Nielsen, Astrid G; Trebbien, Ramona; Poulsen, Mille W; Andersen, Peter H; Fischer, Thea K

2017-06-29

In Europe, enterovirus A71 (EV-A71) has primarily been associated with sporadic cases of neurological disease. The recent emergence of new genotypes and larger outbreaks with severely ill patients demonstrates a potential for the spread of new, highly pathogenic EV-A71 strains. Detection and characterisation of these new emerging EV variants is challenging as standard EV assays may not be adequate, necessitating the use of whole genome analysis. This article is copyright of The Authors, 2017.

A multi-proxy reconstruction of Holocene climate change from Blessberg Cave, Germany

NASA Astrophysics Data System (ADS)

Breitenbach, Sebastian F. M.; Plessen, Birgit; Wenz, Sarah; Leonhardt, Jens; Tjallingii, Rik; Scholz, Denis; Jochum, Klaus Peter; Marwan, Norbert

2016-04-01

Although Holocene climate dynamics were relatively stable compared to glacial conditions, climatic changes had significant impact on ecosystems and human society on various timescales (Mayewski et al. 2004, Donges et al. 2015, Tan et al. 2015). Precious few high-resolution records on Holocene temperature and precipitation conditions in Central Europe are available (e.g., von Grafenstein et al. 1999, Fohlmeister et al. 2012). Here we present a speleothem-based reconstruction of past climate dynamics from Blessberg Cave, Thuringia, central Germany. Three calcitic stalagmites were recovered when the cave was discovered during tunneling operations in 2008. Samples BB-1, -2 and -3 were precisely dated by the 230Th/U-method, with errors between 10 and 160 years (2σ). The combined record covers large parts of the Holocene (10 - 0.4 ka BP). δ13C and δ18O were analysed at 100 μm resolution. To gain additional insights in infiltration conditions, Sr/Ca and S/Ca were measured on BB-1 and BB-3 using an Röntgenanalytik Eagle XXL μXRF scanner. Differences to other central European records (e.g., von Grafenstein et al. 1999, Fohlmeister et al. 2012) suggest complex interaction between multiple factors influencing speleothem δ18O in Blessberg Cave. Furthermore, no clear influence of the North Atlantic Oscillation on our proxies is found. However, a link across the N Atlantic realm is indicated by a centennial-scale correlation between Blessberg δ18O values and minerogenic input into lake SS1220 in Greenland over the last 5 ka (Olsen et al. 2012). In addition, recurrence analysis indicates an imprint of Atlantic Bond events on Blessberg δ18O values (Marwan et al. 2014), corroborating the suggested link with high northern latitudes. Larger runoff into the Greenland lake seems to be associated with lower δ18O, higher δ13C and S/Ca ratios, as well as lower Sr/Ca ratios in Blessberg Cave speleothems. This might be linked to lower local temperature and/or changes in precipitation seasonality. Opposing millennial scale trends with lowering S/Ca ratios and δ13C values but increasing Sr/Ca ratios calls for more than one controlling factor. Most likely, δ13C decreased through the Holocene due to afforestation, which in turn might have increased sulphate retention in the thickening soil cover (Frisia et al. 2005) and limited sulphur flux into the cave. Alternatively, marine sulfur flux could have diminished with winter wind intensities. However, additional data is required to clarify this hypothesis. A positive Sr/Ca trend through the Holocene might result from increasing prior calcite precipitation induced by a negative moisture balance in summer. References Breitenbach et al. (2012) Climate of the Past 8, 1765-1779 Donges et al. (2015) Climate of the Past 11, 709-741 Fohlmeister et al. (2012) Climate of the Past 8, 1751-1764 Frisia et al. (2005) EPSL 235, 729-740 Marwan et al. (2014) Geophysical Research Abstracts 16, EGU2014-8893 Mayewski et al. (2004) Quaternary Research 62, 243-255 Olsen et al. (2012) Nature Geoscience 5, 808-812 Tan et al. (2015) Scientific Reports 5:12284 von Grafenstein et al. (1999) Science 284, 1654-1657

Enhanced removal of hazardous dye form aqueous solutions and real textile wastewater using bifunctional chitin/lignin biosorbent.

PubMed

Wawrzkiewicz, Monika; Bartczak, Przemysław; Jesionowski, Teofil

2017-06-01

A new biomaterial based on chitin and lignin was prepared and applied for the removal of hazardous dye C.I. Direct Blue 71 (DB71) from aqueous solutions and wastewaters. The dye sorption on the chitin/lignin biosorbent (Ch/L) was examined depending on the initial dye concentration (50-200mg/L), phase contact time (1-1440min), kind of auxiliaries (NaCl, Na 2 SO 4 , anionic surfactant SDS) and their concentrations (1-20g/L salts, 0.1-0.75g/L SDS), initial solution pH as well as temperature (20-50°C). The equilibrium and kinetic characteristics of C.I. Direct Blue 71 uptake by chitin/lignin followed by the Freundlich isotherm model and the pseudo-second order model rather than the Langmuir, Tempkin models, and pseudo-first order model. C.I. Direct Blue 71 adsorption on chitin/lignin was spontaneous (-2.86 to -8.14kJ/mol) and endothermic (60.1kJ/mol). The possibilities of dye elution and reuse by means of the batch method were investigated and as follows the chemical reaction is an inseparable sorption mechanism. Purification of wastewaters containing direct dyes was made with 91% efficiency after 1h of phase contact time. For comparison, data obtained or obtained results in the DB71-chitin (Ch) system were also presented. Copyright © 2017 Elsevier B.V. All rights reserved.

Evolution and Spatiotemporal Dynamics of Enterovirus A71 Subgenogroups in Vietnam.

PubMed

Thao, Nguyen Thi Thanh; Donato, Celeste; Trang, Vu Thi Huyen; Kien, Nguyen Trung; Trang, Ph M Mai Thuy; Khanh, Tran Quoc; Nguyet, Dang Thi; Sessions, October M; Cuong, Hoang Quoc; Lan, Phan Trong; Huong, Vu Thi Que; van Doorn, H Rogier; Vijaykrishna, Dhanasekaran

2017-12-12

Enterovirus A71 (EV-A71) is the major cause of severe hand, foot, and mouth disease and viral encephalitis in children across the Asia-Pacific region, including in Vietnam, which has experienced a high burden of disease in recent years. Multiple subgenogroups (C1, C4, C5, and B5) concurrently circulate in the region with a large variation in epidemic severity. The relative differences in their evolution and epidemiology were examined within Vietnam and globally. A total of 752 VP1 gene sequences were analyzed (413 generated in this study combined with 339 obtained from GenBank), collected from patients in 36 provinces in Vietnam during 2003-2013, along with epidemiological metadata. Globally representative VP1 gene datasets of subgenogroups were used to coestimate time-resolved phylogenies and relative genetic diversity to infer virus origins and regional transmission network. Despite frequent virus migration between countries, the highest genetic diversity of individual subgenogroups was maintained independently for several years in specific Asian countries representing genogroup-specific sources of EV-A71 diversity. This study highlights a persistent transmission network of EV-A71, with specific Asian countries seeding other countries in the region and beyond, emphasizing the need for improved EV-A71 surveillance and detailed genetic and antigenic characterization. © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

Plasmaspheric, Faraday and Total Electron Contents, 1977 and 1978.

DTIC Science & Technology

1980-12-01

71 -LLI 𔃿’~-7 COt UI’ 5 ’’’ -’j i S’t It -T -0...8217 iC C 𔃺 C’) 𔃿’ - ’f --- 󈧅 CA 17) i- ’CC,7 L - 0’] 𔄁 r C’) ’i r -r ’tit ck’C ju MS Q 0 up US Un CC’ C’) , X 1: U -1 I ’ ’Xi U ) C’) ’ 71 𔄁 U A ly...C ki4 Cr C. 𔄁.1 -.- - 0i Ci C’) Ut ’it 𔃿’ .7.’ C’ .CC’ L!w II~-j1 ’it -i1 ’.- W 𔃿 ’it s- t WT ’-2_ 𔃿’ T- I ’M IN~ 𔃺 C’) !’

Abstracts of Papers Presented at the Annual Meeting of the Society of General Physiologists (38th) Held at Woods Hole, Massachusetts on 6-9 September 1984.

DTIC Science & Technology

1984-09-09

71. Na*-K Cotransport in Human Red Cells: Reversible Inactivation by Metabolic Depletion N. C. ADRAGNA , C. M. PERKINS,* and P. K. LAUF, Department...Rb*) influx. In 1971, Beauge and Adragna (/. Gem. PhruiL, 57:577) showed that the latter was inhibited by iodoecet- amide and hence inferred its...A., 75 Foskett, K., 44 Adragna , N. C., 71 Freedman, J. C., 41 Alderton, J., 32 Gainer, H., 7, 46 Allen, R. D., I Garcia-Diaz, J. F., 61,66 Alles, W

Whole-genome transcriptional analysis of Escherichia coli during heat inactivation processes related to industrial cooking.

PubMed

Guernec, A; Robichaud-Rincon, P; Saucier, L

2013-08-01

Escherichia coli K-12 was grown to the stationary phase, for maximum physiological resistance, in brain heart infusion (BHI) broth at 37°C. Cells were then heated at 58°C or 60°C to reach a process lethality value \\[\\mathbf{\\left(}{{\\mathit{F}}^{\\mathit{o}}}_{\\mathbf{70}}^{\\mathbf{10}}\\mathbf{\\right)} \\] of 2 or 3 or to a core temperature of 71°C (control industrial cooking temperature). Growth recovery and cell membrane integrity were evaluated immediately after heating, and a global transcription analysis was performed using gene expression microarrays. Only cells heated at 58°C with F(o) = 2 were still able to grow on liquid or solid BHI broth after heat treatment. However, their transcriptome did not differ from that of bacteria heated at 58°C with F(o) = 3 (P value for the false discovery rate [P-FDR] > 0.01), where no growth recovery was observed posttreatment. Genome-wide transcriptomic data obtained at 71°C were distinct from those of the other treatments without growth recovery. Quantification of heat shock gene expression by real-time PCR revealed that dnaK and groEL mRNA levels decreased significantly above 60°C to reach levels similar to those of control cells at 37°C (P < 0.0001). Furthermore, despite similar levels of cell inactivation measured by growth on BHI media after heating, 132 and 8 genes were differentially expressed at 71°C compared to 58°C and 60°C at F(o) = 3, respectively (P-FDR < 0.01). Among them, genes such as aroA, citE, glyS, oppB, and asd, whose expression was upregulated at 71°C, may be worth investigating as good biomarkers for accurately determining the efficiency of heat treatments, especially when cells are too injured to be enumerated using growth media.

Macrosolen bidoupensis (Loranthaceae), a new species from Bidoup Nui Ba National Park, southern Vietnam

PubMed Central

Tagane, Shuichiro; Dang, Van Son; Ngoc, Nguyen Van; Binh, Hoang Thi; Komada, Natsuki; Wai, Jarearnsak Sae; Naiki, Akiyo; Nagamasu, Hidetoshi; Toyama, Hironori; Yahara, Tetsukazu

2017-01-01

Abstract Macrosolen bidoupensis Tagane & V.S.Dang, sp. nov. (Loranthaceae) is newly described from Bidoup Nui Ba National Park in Lam Dong Province, southern Vietnam. The new species is characterized by small broadly elliptic to circular leaves, sessile to short petioles, slightly cordate to rounded leaf bases, 4–5 pairs of lateral veins and a basally green corolla tube. An illustration, a summary of DNA barcoding of the plastid genes rbcL and matK, and a key to the species of Macrosolen in Vietnam are provided. PMID:28781562

Studies on the wintertime current structure and T-S fine-structure in the Taiwan Strait

NASA Astrophysics Data System (ADS)

Hu, Jianyu; Fu, Zilang; Wu, Lianxing

1990-12-01

A cruise through the western sea area of the Taiwan Strait was carried out by the R/V Dong Fang Hong in December, 1987. Eight anchored and 10 not anchored stations were set up. Over 25 time-series current observations were made at each station and CTD (Conductivity-temperature-depth) measurements were made at 5 anchored and 10 not anchored stations. Based on the measured data. fine-structures and step-like vertical structures of temperature and salinity were analysed and a tentative wintertime current structure in the Taiwan Strait was described.

Comparison and Research on New Rural Community Management Patterns of Shan Dong Province

NASA Astrophysics Data System (ADS)

Fang, Lei; Zhang, Xiaomei

Rural community is an important institutional innovation,which has important effect and edification to future new rural management.There are three new rural community management patterns in shandong province:divisions of the village community,many villages community and village merge community. This article not only introduce three models,but also compare them in four aspects: community scale, community management,infrastructure,resource utilization.Pointing out the strength and weakness of three models.Drawing a conclusion that village merge community is the active reaction for rural urbanization. And could be the important recommended breed.

Phytoestrogens: a viable option?

PubMed

Russell, Lori; Hicks, G Swink; Low, Annette K; Shepherd, Jinna M; Brown, C Andrew

2002-10-01

Estrogen replacement therapy is one of the most commonly prescribed medicines in the United States by traditional medical professionals. Over the past decade, the market for complementary/ alternative therapies for hormone replacement has dramatically increased. Women are seeking more "natural" alternatives to treat menopausal symptoms. Well-designed randomized clinical trials are often lacking, as is the information on efficacy and safety. This article will review several popular herbal therapies for menopausal symptoms including phytoestrogens, black cohosh (Cimicifuga racemosa), dong quai (Angelica sinensis), chast tree (Vitex agnus-castus), and wild Mexican yam. Their use, mechanism of action, and adverse effects are outlined.

Rapid screening of commercially available herbal products for the inhibition of major human hepatic cytochrome P450 enzymes using the N-in-one cocktail.

PubMed

Sevior, D K; Hokkanen, J; Tolonen, A; Abass, K; Tursas, L; Pelkonen, O; Ahokas, J T

2010-04-01

Self-administration of complementary products concurrently with conventional medication is increasingly common. The potential for cytochrome P450 (CYP) inhibition requires investigation. The N-in-one assay with ten probe substrates for nine CYPs was used with human liver microsomes to investigate ten products. CYP inhibition was measured in a single liquid chromatography-tandem mass spectrometry (LC/MS-MS) analysis. Estimated IC(50)-values were determined for the extracts that produced significant inhibition (less than 100 microg ml(-1)). Inhibition of CYP2C19 by dong quai (IC(50) = 13.7-14.3 microg ml(-1) for the methanolic extract) and CYP2D6 by goldenseal (IC(50) = 6.7 and 6.3 microg ml(-1) for the aqueous and methanolic extracts, respectively), are of particular concern as the potential for adverse interactions is high. The inhibition of CYP2C8 by horsetail (IC(50) = 93 microg ml(-1) for the aqueous extract) requires further investigation, as the potential for concurrent use with products that require CYP2C8 for metabolism is significant. CYP3A4 inhibition varied depending on the probe reaction being monitored. The earlier reported findings of inhibition by black cohosh, goldenseal and gotu kola were confirmed. The present work has shown that the N-in-one cocktail is a rapid and reliable method that can be used as an initial screen to help prioritize products that require more detailed investigations and it can also be applied to monitor product variability.

Discovery of CREBBP Bromodomain Inhibitors by High-Throughput Docking and Hit Optimization Guided by Molecular Dynamics.

PubMed

Xu, Min; Unzue, Andrea; Dong, Jing; Spiliotopoulos, Dimitrios; Nevado, Cristina; Caflisch, Amedeo

2016-02-25

We have identified two chemotypes of CREBBP bromodomain ligands by fragment-based high-throughput docking. Only 17 molecules from the original library of two-million compounds were tested in vitro. Optimization of the two low-micromolar hits, the 4-acylpyrrole 1 and acylbenzene 9, was driven by molecular dynamics results which suggested improvement of the polar interactions with the Arg1173 side chain at the rim of the binding site. The synthesis of only two derivatives of 1 yielded the 4-acylpyrrole 6 which shows a single-digit micromolar affinity for the CREBBP bromodomain and a ligand efficiency of 0.34 kcal/mol per non-hydrogen atom. Optimization of the acylbenzene hit 9 resulted in a series of derivatives with nanomolar potencies, good ligand efficiency and selectivity (see Unzue, A.; Xu, M.; Dong, J.; Wiedmer, L.; Spiliotopoulos, D.; Caflisch, A.; Nevado, C.Fragment-Based Design of Selective Nanomolar Ligands of the CREBBP Bromodomain. J. Med. Chem. 2015, DOI: 10.1021/acs.jmedchem.5b00172). The in silico predicted binding mode of the acylbenzene derivative 10 was validated by solving the structure of the complex with the CREBBP bromodomain.

Variance Function Estimation. Revision.

DTIC Science & Technology

1987-03-01

UNLSIFIED RFOSR-TR-87-±112 F49620-85-C-O144 F/C 12/3 NL EEEEEEh LOUA28~ ~ L53 11uLoo MICROOP REOUINTS-’HR ------ N L E U INARF-% - IS %~1 %i % 0111...and 9 jointly. If 7,, 0. and are any preliminary estimators for 71, 6. and 3. define 71 and 6 to be the solutions of (4.1) N1 IN2 (7., ’ Td " ~ - / =0P

36 CFR 1280.71 - What are the general rules for using NARA property in the Washington, DC, area?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false What are the general rules for using NARA property in the Washington, DC, area? 1280.71 Section 1280.71 Parks, Forests, and..., B, and C of this part, you must adhere to the following rules when using NARA public spaces: (a) All...

36 CFR 1280.71 - What are the general rules for using NARA property in the Washington, DC, area?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false What are the general rules for using NARA property in the Washington, DC, area? 1280.71 Section 1280.71 Parks, Forests, and..., B, and C of this part, you must adhere to the following rules when using NARA public spaces: (a) All...

Molecular Comparison and Evolutionary Analyses of VP1 Nucleotide Sequences of New African Human Enterovirus 71 Isolates Reveal a Wide Genetic Diversity

PubMed Central

Nougairède, Antoine; Joffret, Marie-Line; Deshpande, Jagadish M.; Dubot-Pérès, Audrey; Héraud, Jean-Michel

2014-01-01

Most circulating strains of Human enterovirus 71 (EV-A71) have been classified primarily into three genogroups (A to C) on the basis of genetic divergence between the 1D gene, which encodes the VP1 capsid protein. The aim of the present study was to provide further insights into the diversity of the EV-A71 genogroups following the recent description of highly divergent isolates, in particular those from African countries, including Madagascar. We classified recent EV-A71 isolates by a large comparison of 3,346 VP1 nucleotidic sequences collected from GenBank. Analysis of genetic distances and phylogenetic investigations indicated that some recently-reported isolates did not fall into the genogroups A-C and clustered into three additional genogroups, including one Indian genogroup (genogroup D) and 2 African ones (E and F). Our Bayesian phylogenetic analysis provided consistent data showing that the genogroup D isolates share a recent common ancestor with the members of genogroup E, while the isolates of genogroup F evolved from a recent common ancestor shared with the members of the genogroup B. Our results reveal the wide diversity that exists among EV-A71 isolates and suggest that the number of circulating genogroups is probably underestimated, particularly in developing countries where EV-A71 epidemiology has been poorly studied. PMID:24598878

Antiviral activities of peptide-based covalent inhibitors of the Enterovirus 71 3C protease

PubMed Central

Tan, Yong Wah; Ang, Melgious Jin Yan; Lau, Qiu Ying; Poulsen, Anders; Ng, Fui Mee; Then, Siew Wen; Peng, Jianhe; Hill, Jeffrey; Hong, Wan Jin; Chia, Cheng San Brian; Chu, Justin Jang Hann

2016-01-01

Hand, Foot and Mouth Disease is a highly contagious disease caused by a range of human enteroviruses. Outbreaks occur regularly, especially in the Asia-Pacific region, putting a burden on public healthcare systems. Currently, there is no antiviral for treating this infectious disease and the only vaccines are limited to circulation in China, presenting an unmet medical need that needs to be filled urgently. The human enterovirus 3 C protease has been deemed a plausible drug target due to its essential roles in viral replication. In this study, we designed and synthesized 10 analogues of the Rhinovirus 3 C protease inhibitor, Rupintrivir, and tested their 3 C protease inhibitory activities followed by a cellular assay using human enterovirus 71 (EV71)-infected human RD cells. Our results revealed that a peptide-based compound containing a trifluoromethyl moiety to be the most potent analogue, with an EC50 of 65 nM, suggesting its potential as a lead for antiviral drug discovery. PMID:27645381

Self-defense of Large Aircraft

DTIC Science & Technology

2008-03-01

29 b. Ultraviolet .................................................................... 34 c. Laser ...50 4. Laser ...69 C. LASER WARNING RECEIVERS ....................................................... 71 ix 1. Propagation

The heptad repeats region is essential for AcMNPV P10 filament formation and not the proline-rich or the C-terminus basic regions.

PubMed

Dong, Chunsheng; Deng, Fei; Li, Dan; Wang, Hualin; Hu, Zhihong

2007-09-01

Baculovirus P10 protein is a small conserved protein and is expressed as bundles of filaments in the host cell during the late phase of virus infection. So far the published results on the domain responsible for filament structural formation have been contradictory. Electron microscopy revealed that the C-terminus basic region was involved in filament structural formation in the Autographa californica multiple nucleocapsid nucleopolyhedrovirus (AcMNPV) [van Oers, M.M., Flipsen, J.T., Reusken, C.B., Sliwinsky, E.L., Vlak, J.M., 1993. Functional domains of the p10 protein of Autographa californica nuclear polyhedorsis virus. J. Gen. Virol. 74, 563-574.]. While in the Helicoverpa armigera nucleopolyhedrovirus (HearNPV), the heptad repeats region but not the C-terminus domain was proven to be responsible for filament formation [Dong, C., Li, D., Long, G., Deng, F., Wang, H., Hu, Z., 2005. Identification of functional domains required for HearNPV P10 filament formation. Virology 338, 112-120.]. In this manuscript, fluorescence confocal microscopy was applied to study AcMNPV P10 filament formation. A set of plasmids containing different P10 structural domains fused with a fluorescent protein were constructed and transfected into Sf-9 cells. The data indicated that the heptad repeats region, but not the proline-rich region or the C-terminus basic region, is essential for AcMNPV P10 filament formation. Co-transfection of P10s tagged with different fluorescent revealed that P10s with defective heptad repeats region could not interact with intact heptad repeats region or even full-length P10s to form filament structure. Within the heptad repeats region, deletion of the three amino acids spacing of AcMNPV P10 appeared to have no significant impact on the formation of filament structures, but the content of the heptad repeats region appeared to play a role in the morphology of the filaments.

Construction of "Toxin Complex" in a Mutant Serotype C Strain of Clostridium botulinum Harboring a Defective Neurotoxin Gene.

PubMed

Suzuki, Tomonori; Nagano, Thomas; Niwa, Koichi; Uchino, Masataka; Tomizawa, Motohiro; Sagane, Yoshimasa; Watanabe, Toshihiro

2017-01-01

A non-toxigenic mutant of the toxigenic serotype C Clostridium botulinum strain Stockholm (C-St), C-N71, does not produce the botulinum neurotoxin (BoNT). However, the original strain C-St produces botulinum toxin complex, in which BoNT is associated with non-toxic non-hemagglutinin (NTNHA) and three hemagglutinin proteins (HA-70, HA-33, and HA-17). Therefore, in this study, we aimed to elucidate the effects of bont gene knockout on the formation of the "toxin complex." Nucleotide sequence analysis revealed that a premature stop codon was introduced in the bont gene, whereas other genes were not affected by this mutation. Moreover, we successfully purified the "toxin complex" produced by C-N71. The "toxin complex" was identified as a mixture of NTNHA/HA-70/HA-17/HA-33 complexes with intact NTNHA or C-terminally truncated NTNHA, without BoNT. These results indicated that knockout of the bont gene does not affect the formation of the "toxin complex." Since the botulinum toxin complex has been shown to play an important role in oral toxin transport in the human and animal body, a non-neurotoxic "toxin complex" of C-N71 may be valuable for the development of an oral drug delivery system.

Antibodies to B7.1 define the GFCC'C" face of the N-terminal domain as critical for co-stimulatory interactions.

PubMed

Wang, Suyue; Veldman, Geertruida M; Stahl, Mark; Xing, Yuzhe; Tobin, James F; Erbe, David V

2002-09-02

Antagonists of the B7 family of co-stimulatory molecules have the potential for altering immune responses therapeutically. To better define the requirements for such inhibitors, we have mapped the binding of an entire panel of blocking antibodies specific for human B7.1. By mutagenesis, each of the residues critical for blocking antibody binding appeared to fall entirely within the N-terminal V-set domain of B7.1. Thus, although antibody-antigen interacting surfaces can be quite large, these results indicate that a relatively small portion of the GFCC'C" face of this domain is crucial for further antagonist development.

An eight-year prospective controlled study about the safety and diagnostic value of cardiac and non-cardiac 1.5-T MRI in patients with a conventional pacemaker or a conventional implantable cardioverter defibrillator.

PubMed

Lupo, Pierpaolo; Cappato, Riccardo; Di Leo, Giovanni; Secchi, Francesco; Papini, Giacomo D E; Foresti, Sara; Ali, Hussam; De Ambroggi, Guido M G; Sorgente, Antonio; Epicoco, Gianluca; Cannaò, Paola M; Sardanelli, Francesco

2018-06-01

To investigate safety and diagnostic value of 1.5-T MRI in carriers of conventional pacemaker (cPM) or conventional implantable defibrillator (cICD). We prospectively compared cPM/cICD-carriers undergoing MRI (study group, SG), excluding those device-dependent or implanted <6 weeks before enrolment or prior to 01/01/2000, with cPM/cICD-carriers undergoing chest x-ray, CT or follow-up (reference group, RG). 142 MRI (55 cardiac) were performed in 120 patients with cPM (n=71) or cICD (n=71). In the RG 98 measurements were performed in 95 patients with cPM (n=40) or cICD (n=58). No adverse events were observed. No MRI prolonged/interrupted. All cPM/cICD were correctly reprogrammed after MRI without malfunctions. One temporary communication failure was observed in one cPM-carrier. Immediately after MRI, 12/14 device interrogation parameters did not change significantly (clinically negligible changes of battery voltage and cICD charging time), without significant variations for SG versus RG. Three-12 months after MRI, 9/11 device interrogation parameters did not change significantly (clinically negligible changes of battery impedance/voltage). Non-significant changes of three markers of myocardial necrosis. Non-cardiac MRI: 82/87 diagnostic without artefacts; 4/87 diagnostic with artefacts; 1/87 partially diagnostic. Cardiac MRI: in cPM-carriers, 14/15 diagnostic with artefacts, 1/15 partially diagnostic; in cICD-carriers, 9/40 diagnostic with artefacts, 22 partially diagnostic, nine non-diagnostic. A favourable risk-benefit ratio of 1.5-T MRI in cPM/cICD carriers was reported. • Cooperation between radiologists and cardiac electrophysiologists allowed safe 1.5-T MRI in cPM/cICD-carriers. • No adverse events for 142 MRI in 71 cPM-carriers and 71 cICD-carriers. • Ninety-nine per cent (86/87) of non-cardiac MRI in cPM/cICD-carriers were diagnostic. • All cPM-carrier cardiac MRIs had artefacts, 14 examinations diagnostic, 1 partially diagnostic. • Twenty-three per cent (9/40) of cardiac MRI in cICD-carriers were non-diagnostic.

[Stimuli phrases of adductor spasmodic dysphonia phonatory break in mandarin Chinese].

PubMed

Ge, Pingjiang; Ren, Qingyi; Chen, Zhipeng; Cheng, Qiuhui; Sheng, Xiaoli; Wang, Ling; Chen, Shaohua; Zhang, Siyi

2015-12-01

To investigate the characteristics of adductor spasmodic dysphonia phonatory break in mandarin Chinese and select the stimuli phrases. Thirty-eight patients with adductor spasmodic dysphonia were involved in this study. Standard phrase " fù mŭ xīn" and a speech corpus in mandarin Chinese with 229 syllables covering all vowel and constant of mandarin Chinese were selected. Every patient read the phrases above twice in normal speed and comfortable voice. Two auditory perpetual speech pathologists marked phonatory break syllables respectively. The frequency of phonatory break syllables and their located phrases were calculated, rated and described. The phrases including the most phonatory break syllables were selected as stimuli phrases, the phonatory break frequency of which was also higher than that of standard phrase "fù mŭ xīn". Phonatory break happened in the reading of all patients. The average number of phonatory break syllables was 14 (3-33). Phonatroy break occurred when saying 177 (77.3%) syllables in the speech corpus. The syllables "guŏ, rén, zāng, diàn, chē, gè, guăn, a, bā, ne, de" broke in 23.1%-41.0% patients. These syllables belonged to the phrases "pĭng guŏ, huŏ chē, shì de, nĭ shì gè hăo rén, wŏ mén shì yŏu zŏng shì bă qĭn shì nong dé hĕn zāng, wŏ mén nà biān yŏu wăng qiú yùn dong chăng, cān gŭan, jiŭ bā hé yī gè miàn bāo dìan, tā shì duō me kāng kăi a,wŏ yīng gāi zài xìn lĭ xiĕ yī xiē shén mē ne?". Thirty-seven patients (97.3%) had phonatory break in above mentioned words. Ratios of these words phonatory break also were more than "fù mŭ xīn". Adductor spasmodic dysphonic patients exhibited different degrees of phonatory break in mandarine Chinese. The phrases" shì de, pĭng guŏ, huŏ chē, nĭ shì gè hăo rén, wŏ mén nà biān yŏu wăng qiú yùn dong chăng, cān gŭan, jiŭ bā hé yī gè miàn bāo dìan, tā shì duō me kāng kăi a" were recommended as stimuli phrases for adductor spasmodic dysphonia evaluation.

Final report : oceanic surveillance and navigation analysis, FY 71.

DOT National Transportation Integrated Search

1971-06-01

The report summarizes the oceanic surveillance and navigation analysis performed at Transportation Systems Center under PPA FA-04 for FY 71. Three major efforts are reviewed and discussed herein: summary of the North Atlantic Systems Planning Group c...

A path-dependent fatigue crack propagation model under non-proportional modes I and III loading conditions

DOE PAGES

Mei, J.; Dong, P.; Kalnaus, S.; ...

2017-07-21

It has been well established that fatigue damage process is load-path dependent under non-proportional multi-axial loading conditions. Most of studies to date have been focusing on interpretation of S-N based test data by constructing a path-dependent fatigue damage model. Our paper presents a two-parameter mixed-mode fatigue crack growth model which takes into account of crack growth dependency on both load path traversed and a maximum effective stress intensity attained in a stress intensity factor plane (e.g.,KI-KIII plane). Furthermore, by taking advantage of a path-dependent maximum range (PDMR) cycle definition (Dong et al., 2010; Wei and Dong, 2010), the two parametersmore » are formulated by introducing a moment of load path (MLP) based equivalent stress intensity factor range (ΔKNP) and a maximum effective stress intensity parameter KMax incorporating an interaction term KI·KIII. To examine the effectiveness of the proposed model, two sets of crack growth rate test data are considered. The first set is obtained as a part of this study using 304 stainless steel disk specimens subjected to three combined non-proportional modes I and III loading conditions (i.e., with a phase angle of 0°, 90°, and 180°). The second set was obtained by Feng et al. (2007) using 1070 steel disk specimens subjected to similar types of non-proportional mixed-mode conditions. Once the proposed two-parameter non-proportional mixed-mode crack growth model is used, it is shown that a good correlation can be achieved for both sets of the crack growth rate test data.« less

A path-dependent fatigue crack propagation model under non-proportional modes I and III loading conditions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mei, J.; Dong, P.; Kalnaus, S.

It has been well established that fatigue damage process is load-path dependent under non-proportional multi-axial loading conditions. Most of studies to date have been focusing on interpretation of S-N based test data by constructing a path-dependent fatigue damage model. Our paper presents a two-parameter mixed-mode fatigue crack growth model which takes into account of crack growth dependency on both load path traversed and a maximum effective stress intensity attained in a stress intensity factor plane (e.g.,KI-KIII plane). Furthermore, by taking advantage of a path-dependent maximum range (PDMR) cycle definition (Dong et al., 2010; Wei and Dong, 2010), the two parametersmore » are formulated by introducing a moment of load path (MLP) based equivalent stress intensity factor range (ΔKNP) and a maximum effective stress intensity parameter KMax incorporating an interaction term KI·KIII. To examine the effectiveness of the proposed model, two sets of crack growth rate test data are considered. The first set is obtained as a part of this study using 304 stainless steel disk specimens subjected to three combined non-proportional modes I and III loading conditions (i.e., with a phase angle of 0°, 90°, and 180°). The second set was obtained by Feng et al. (2007) using 1070 steel disk specimens subjected to similar types of non-proportional mixed-mode conditions. Once the proposed two-parameter non-proportional mixed-mode crack growth model is used, it is shown that a good correlation can be achieved for both sets of the crack growth rate test data.« less

Geological constraints on cave development in the plateau-gorge karst of South China (Wulong, Chongqing)

NASA Astrophysics Data System (ADS)

Szczygieł, Jacek; Golicz, Mateusz; Hercman, Helena; Lynch, Erin

2018-03-01

The Houping Tiankeng cluster is a part of the South China Karst UNESCO World Natural Heritage Site. Within the distinctive Wulong plateau-gorge karst, > 200 km of cave passages have been documented to date. This paper focuses on detailed tectonic and morphological research on the Luo Shui Kong cave, enriched with U-series dating of speleothems and complemented by morphometric analysis of the San Wang Dong and Er Wang Dong caves. All of these caves exhibit three regional levels of cave development: 1) 1040-1020 m a.s.l.; 2) 900-840 m a.s.l.; and 3) 740-660 m a.s.l. The Houping Tiankeng area is a carbonate rock sequence several hundred meters thick, overlain by the less soluble Lower Ordovician strata, limiting recharge points to faults exposing underlying easily soluble formations. This leads to the domination of concentrated, high-volume inflow and thus results in caves of large volume in the plateau-gorge karst. Shafts connecting the surface with cave passages located underneath formed along faults, changing the hydrogeological pattern through karst water capture and remodeling of existing conduits, albeit mainly by increasing their overall dimensions rather than by deepening them. The most favorable structures for cave-level development are two sets of joints conjugated with gently inclined bedding. Since these joints are characterized by a small vertical extent, downward development is limited. Hence, most of the passages are wide but not deep canyons and typical of a water-table cave pattern. Places where the fault plane is eroded from the surface and where, at the same time, an underneath cave chamber ceiling expands upwards are particularly predisposed to the formation of a tiankeng.

Characterizing Enterovirus 71 and Coxsackievirus A16 virus-like particles production in insect cells.

PubMed

Somasundaram, Balaji; Chang, Cindy; Fan, Yuan Y; Lim, Pei-Yin; Cardosa, Jane; Lua, Linda

2016-02-15

Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) are two viruses commonly responsible for hand, foot and mouth disease (HFMD) in children. The lack of prophylactic or therapeutic measures against HFMD is a major public health concern. Insect cell-based EV71 and CVA16 virus-like particles (VLPs) are promising vaccine candidates against HFMD and are currently under development. In this paper, the influence of insect cell line, incubation temperature, and serial passaging effect and stability of budded virus (BV) stocks on EV71 and CVA16 VLP production was investigated. Enhanced EV71 and CVA16 VLP production was observed in Sf9 cells compared to High Five™ cells. Lowering the incubation temperature from the standard 27°C to 21°C increased the production of both VLPs in Sf9 cells. Serial passaging of CVA16 BV stocks in cell culture had a detrimental effect on the productivity of the structural proteins and the effect was observed with only 5 passages of BV stocks. A 2.7× higher production yield was achieved with EV71 compared to CVA16. High-resolution asymmetric flow field-flow fractionation couple with multi-angle light scattering (AF4-MALS) was used for the first time to characterize EV71 and CVA16 VLPs, displaying an average root mean square radius of 15±1nm and 15.3±5.8 nm respectively. This study highlights the need for different approaches in the design of production process to develop a bivalent EV71 and CVA16 vaccine. Copyright © 2015 Elsevier Inc. All rights reserved.

Subbanding, Charge Transport and Related Applications in Semiconductor Devices.

DTIC Science & Technology

1977-10-01

3 1) .7., -, ŝ .2 437. 9 7 54.2 •0 373" 11 C 15 12E. 10 .G 4. Vi ? c .11,5i.. 2 - 9 t,5.5 . 1 05 11 .752;’L 10 .3󈧭)- 10 .11󈧰 4 10 .- ,:C2 9 7...1,- 4 -72. 53,* 7 3-5 71. 1u 3 o6.L13 2 .77d 333. ’.id 471. :j J 7. VI 1.8 71.793 05.566 199.991 335-027 470.315 o05 .753 .2. 1 72.560 o5. 029 199...6;7 ;20430E 2 .20430E 2 ;23430E 2 .20433E 2 .20430! 2 VI ;20430E 2 ;20430E 2 ;20430E 2 ;20433E 2 -20430! 2 9;8 ;20430E 2 ;20430E 2 ;20430E 2 .20430E 2

Sphingopyxis italica sp. nov., isolated from Roman catacombs.

PubMed

Alias-Villegas, Cynthia; Jurado, Valme; Laiz, Leonila; Saiz-Jimenez, Cesareo

2013-07-01

A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, strain SC13E-S71(T), was isolated from tuff, volcanic rock, where the Roman catacombs of Saint Callixtus in Rome, Italy, was excavated. Analysis of 16S rRNA gene sequences revealed that strain SC13E-S71(T) belongs to the genus Sphingopyxis, and that it shows the greatest sequence similarity with Sphingopyxis chilensis DSM 14889(T) (98.72 %), Sphingopyxis taejonensis DSM 15583(T) (98.65 %), Sphingopyxis ginsengisoli LMG 23390(T) (98.16 %), Sphingopyxis panaciterrae KCTC 12580(T) (98.09 %), Sphingopyxis alaskensis DSM 13593(T) (98.09 %), Sphingopyxis witflariensis DSM 14551(T) (98.09 %), Sphingopyxis bauzanensis DSM 22271(T) (98.02 %), Sphingopyxis granuli KCTC 12209(T) (97.73 %), Sphingopyxis macrogoltabida KACC 10927(T) (97.49 %), Sphingopyxis ummariensis DSM 24316(T) (97.37 %) and Sphingopyxis panaciterrulae KCTC 22112(T) (97.09 %). The predominant fatty acids were C18 : 1ω7c, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), C14 : 0 2-OH and C16 : 0. The predominant menaquinone was MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. These chemotaxonomic data are common to members of the genus Sphingopyxis. However, a polyphasic approach using physiological tests, DNA base ratios, DNA-DNA hybridization and 16S rRNA gene sequence comparisons showed that the isolate SC13E-S71(T) belongs to a novel species within the genus Sphingopyxis, for which the name Sphingopyxis italica sp. nov. is proposed. The type strain is SC13E-S71(T) ( = DSM 25229(T) = CECT 8016(T)).

Tailorable PC71 BM Isomers: Using the Most Prevalent Electron Acceptor to Obtain High-Performance Polymer Solar Cells.

PubMed

Zhan, Xin-Xing; Zhang, Xin; Dai, Si-Min; Li, Shu-Hui; Lu, Xu-Zhai; Deng, Lin-Long; Xie, Su-Yuan; Huang, Rong-Bin; Zheng, Lan-Sun

2016-12-23

Despite being widely used as electron acceptor in polymer solar cells, commercially available PC 71 BM (phenyl-C 71 -butyric acid methyl ester) usually has a "random" composition of mixed regioisomers or stereoisomers. Here PC 71 BM has been isolated into three typical isomers, α-, β 1 - and β 2 -PC 71 BM, to establish the isomer-dependent photovoltaic performance on changing the ternary composition of α-, β 1 - and β 2 -PC 71 BM. Mixing the isomers in a ratio of α/β 1 /β 2 =8:1:1 resulted in the best power conversion efficiency (PCE) of 7.67 % for the polymer solar cells with PTB7:PC 71 BM as photoactive layer (PTB7=poly[[4,8-bis[(2-ethylhexyl)oxy]benzo[1,2-b:4,5-b']dithiophene-2,6-diyl][3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno[3,4-b]thiophenediyl

Effect of frozen storage, different thawing methods and cooking processes on the survival of Salmonella spp. and Escherichia coli O157:H7 in commercially shaped beef patties.

PubMed

Manios, Stavros G; Skandamis, Panagiotis N

2015-03-01

The effect of common handling practices (i.e., freezing, thawing and cooking) of beef patties on the survival of Salmonella spp. and Escherichia coli O157:H7, was evaluated. Inoculated ground beef was stored at -22 °C for 5 and 75 days. After thawing at 4 °C/16 h, 20 °C/12 h, in microwave/22-24 min, or without prior thawing, beef patties (90 g) were shaped and cooked in oven-broiler or in pan-grill to internal temperatures of 60 °C or 71 °C. Cooking in oven-broiler was more effective compared to pan-grill, especially when cooked to 71 °C. Defrosting methods did not affect significantly (P ≥ 0.05) the survival of the pathogens during subsequent cooking. Frozen storage for 75 days enhanced the survival of E. coli O157:H7, as the pathogen survived 3.1 logCFU/g when cooked in oven-broiler at 71 °C. Results may supplement the existing guidelines for the appropriate practices, associated with freezing, thawing and cooking of patties in households or catering services. Copyright © 2014 Elsevier Ltd. All rights reserved.

Raman scattering evidence of hydrohalite formation on frozen yeast cells.

PubMed

Okotrub, K A; Surovtsev, N V

2013-02-01

We studied yeast cells in physiological solution during freezing by Raman microspectroscopy technique. The purpose was to find out the origin of a sharp peak near ∼3430cm(-1) in Raman spectrum of frozen mammalian cells, observed earlier (J. Dong et al., Biophys. J. 99 (2010) 2453), which presumably could be used as an indicator of intracellar ice appearance. We have shown that this line (actually doublet of 3408 and 3425cm(-1)) corresponds to Raman spectrum of hydrohalite (NaCl⋅2H(2)O), which is formed as the result of the eutectic crystallization of the liquid solution around the cells. We also show that the spatial distribution of hydrohalite in the sample significantly depends on the cooling rate. At lower cooling rate (1°C/min), products of eutectic crystallization form layer on the cell surface which thickness varies for different cells and can reach ∼1μm in thickness. At higher cooling rate (20°C/min), the hydrohalite distribution appears more homogeneous, in the sample, and the eutectic crystallization layer around the cells was estimated to be less than ∼20nm. These experimental results are consistent with scenarios predicted by the two-factor hypothesis for freezing induced cell injury. This work demonstrates a potential of Raman microspectroscopy to study peculiarities of the eutectic crystallization around single cells in vivo with the high spatial resolution. Copyright © 2012 Elsevier Inc. All rights reserved.

Detection of Dystrophin Dp71 in Human Skeletal Muscle Using an Automated Capillary Western Assay System.

PubMed

Kawaguchi, Tatsuya; Niba, Emma Tabe Eko; Rani, Abdul Qawee Mahyoob; Onishi, Yoshiyuki; Koizumi, Makoto; Awano, Hiroyuki; Matsumoto, Masaaki; Nagai, Masashi; Yoshida, Shinobu; Sakakibara, Sachiko; Maeda, Naoyuki; Sato, Osamu; Nishio, Hisahide; Matsuo, Masafumi

2018-05-23

Dystrophin Dp71 is one of the isoforms produced by the DMD gene which is mutated in patients with Duchenne muscular dystrophy (DMD). Although Dp71 is expressed ubiquitously, it has not been detected in normal skeletal muscle. This study was performed to assess the expression of Dp71 in human skeletal muscle. Human skeletal muscle RNA and tissues were obtained commercially. Mouse skeletal muscle was obtained from normal and DMD mdx mice. Dp71 mRNA and protein were determined by reverse-transcription PCR and an automated capillary Western assay system, the Simple Western, respectively. Dp71 was over-expressed or suppressed using a plasmid expressing Dp71 or antisense oligonucleotide, respectively. Full-length Dp71 cDNA was PCR amplified as a single product from human skeletal muscle RNA. A ca. 70 kDa protein peak detected by the Simple Western was determined as Dp71 by over-expressing Dp71 in HEK293 cells, or suppressing Dp71 expression with antisense oligonucleotide in rhabdomyosarcoma cells. The Simple Western assay detected Dp71 in the skeletal muscles of both normal and DMD mice. In human skeletal muscle, Dp71 was also detected. The ratio of Dp71 to vinculin of human skeletal muscle samples varied widely, indicating various levels of Dp71 expression. Dp71 protein was detected in human skeletal muscle using a highly sensitive capillary Western blotting system.

B7-2 Expressed on EL4 Lymphoma Suppresses Antitumor Immunity by an Interleukin 4–dependent Mechanism

PubMed Central

Stremmel, C.; Greenfield, E.A.; Howard, E.; Freeman, G.J.; Kuchroo, V.K.

1999-01-01

For T cells to become functionally activated they require at least two signals. The B7 costimulatory molecules B7-1 and B7-2 provide the “second signal” pivotal for T cell activation. In this report, we studied the relative roles of B7-1 and B7-2 molecules in the induction of antitumor immunity to the T cell thymoma, EL4. We generated EL4 tumor cells that expressed B7-1, B7-2, and B7-1+B7-2 by transfecting murine cDNAs. Our results demonstrate that EL4–B7-1 cells are completely rejected in syngeneic mice. Unlike EL4–B7-1 cells, we find that EL4–B7-2 cells are not rejected but progressively grow in the mice. A B7-1– and B7-2–EL4 double transfectant was generated by introducing B7-2 cDNA into the EL4–B7-1 tumor line that regressed in vivo. The EL4–B7-1+B7-2 double transfectant was not rejected when implanted into syngeneic mice but progressively grew to produce tumors. The double transfectant EL4 cells could costimulate T cell proliferation that could be blocked by anti–B7-1 antibodies, anti–B7-2 antibodies, or hCTLA4 immunoglobulin, showing that the B7-1 and B7-2 molecules expressed on the EL4 cells were functional. In vivo, treatment of mice implanted with double-transfected EL4 cells with anti–B7-2 monoclonal antibody resulted in tumor rejection. Furthermore, the EL4–B7-2 and EL4–B7-1+B7-2 cells, but not the wild-type EL4 cells, were rejected in interleukin 4 (IL-4) knockout mice. Our data suggests that B7-2 expressed on some T cell tumors inhibits development of antitumor immunity, and IL-4 appears to play a critical role in abrogation of the antitumor immune response. PMID:10075975

B7-2 expressed on EL4 lymphoma suppresses antitumor immunity by an interleukin 4-dependent mechanism.

PubMed

Stremmel, C; Greenfield, E A; Howard, E; Freeman, G J; Kuchroo, V K

1999-03-15

For T cells to become functionally activated they require at least two signals. The B7 costimulatory molecules B7-1 and B7-2 provide the "second signal" pivotal for T cell activation. In this report, we studied the relative roles of B7-1 and B7-2 molecules in the induction of antitumor immunity to the T cell thymoma, EL4. We generated EL4 tumor cells that expressed B7-1, B7-2, and B7-1+B7-2 by transfecting murine cDNAs. Our results demonstrate that EL4-B7-1 cells are completely rejected in syngeneic mice. Unlike EL4-B7-1 cells, we find that EL4-B7-2 cells are not rejected but progressively grow in the mice. A B7-1- and B7-2-EL4 double transfectant was generated by introducing B7-2 cDNA into the EL4-B7-1 tumor line that regressed in vivo. The EL4-B7-1+B7-2 double transfectant was not rejected when implanted into syngeneic mice but progressively grew to produce tumors. The double transfectant EL4 cells could costimulate T cell proliferation that could be blocked by anti-B7-1 antibodies, anti-B7-2 antibodies, or hCTLA4 immunoglobulin, showing that the B7-1 and B7-2 molecules expressed on the EL4 cells were functional. In vivo, treatment of mice implanted with double-transfected EL4 cells with anti-B7-2 monoclonal antibody resulted in tumor rejection. Furthermore, the EL4-B7-2 and EL4-B7-1+B7-2 cells, but not the wild-type EL4 cells, were rejected in interleukin 4 (IL-4) knockout mice. Our data suggests that B7-2 expressed on some T cell tumors inhibits development of antitumor immunity, and IL-4 appears to play a critical role in abrogation of the antitumor immune response.

cis-Bis(O-methyl-dithio-carbonato-κ(2) S,S')bis-(tri-phenyl-phosphane-κP)ruthenium(II).

PubMed

Valerio-Cárdenas, Cintya; Hernández-Ortega, Simón; Reyes-Martínez, Reyna; Morales-Morales, David

2013-01-01

In the title compound, [Ru(CH3OCS2)2(C18H15P)2], the Ru(II) atom is in a distorted octa-hedral coordination by two xanthate anions (CH3OCS2) and two tri-phenyl-phosphane (PPh3) ligands. Both bidentate xanthate ligands coordinate the Ru(II) atom with two slightly different Ru-S bond lengths but with virtually equal bite angles [71.57 (4) and 71.58 (3)°]. The packing of the complexes is assured by C-H⋯O and C-H⋯π inter-actions.

7 CFR 1219.71 - Suspension or termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HASS AVOCADO PROMOTION, RESEARCH, AND INFORMATION Hass Avocado Promotion, Research, and Information Order Miscellaneous § 1219.71..., have been engaged in the production or importation of Hass avocados. (c) If, as a result of a...

7 CFR 1219.71 - Suspension or termination.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HASS AVOCADO PROMOTION, RESEARCH, AND INFORMATION Hass Avocado Promotion, Research, and Information Order Miscellaneous § 1219.71..., have been engaged in the production or importation of Hass avocados. (c) If, as a result of a...

7 CFR 1219.71 - Suspension or termination.

Code of Federal Regulations, 2012 CFR

2012-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HASS AVOCADO PROMOTION, RESEARCH, AND INFORMATION Hass Avocado Promotion, Research, and Information Order Miscellaneous § 1219.71..., have been engaged in the production or importation of Hass avocados. (c) If, as a result of a...

7 CFR 1219.71 - Suspension or termination.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HASS AVOCADO PROMOTION, RESEARCH, AND INFORMATION Hass Avocado Promotion, Research, and Information Order Miscellaneous § 1219.71..., have been engaged in the production or importation of Hass avocados. (c) If, as a result of a...

7 CFR 1219.71 - Suspension or termination.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HASS AVOCADO PROMOTION, RESEARCH, AND INFORMATION Hass Avocado Promotion, Research, and Information Order Miscellaneous § 1219.71..., have been engaged in the production or importation of Hass avocados. (c) If, as a result of a...

Official Guard and Reserve Manpower Strengths and Statistics. FY 2000 Summary

DTIC Science & Technology

2000-01-01

t- 0- at UJ UJ 1/1 oo X LULO > < o Z r- u. or O O Z O. < a: oe S»5 < r- O corvi mo -q-m HHfnSin^ Is-. r** rococo m...1- r^ •=!•-ŗ-•<!• rH rHCTirH to »T O rsi Tfrgr^rH ino rH *j- I— iH m m rsirHinm ro rsi rH rsi COCO Lnoo -1 <r m LnoOf\\IC7l rococo r-^ 1- TJ...i a. t i 1 cn > lOolOIN^ioooooolcoHHrrnj-ttiALOLDiflfnOcoOHHONNO rococo in inmioi£)CT1n-i^-inco CTCOOOCOCOOOCOOOCOCOC71CTlCTlCTlC71C71CT

Preface: Ecosystem services, ecosystem health and human communities

NASA Astrophysics Data System (ADS)

Plag, Hans-Peter

2018-04-01

This special issue contains a collection of manuscripts that were originally intended to be included in the special issue on "Physics and Economics of Ecosystem Services Flows" (Volume 101, guest editors H. Su, J. Dong and S. Nagarajan) and "Biogeochemical Processes in the Changing Wetland Environment" (Volume 103, guest editors J. Bai, L. Huang and H. Gao). All of them are addressing issues related to ecosystem services in different settings. Ecosystem services are of high value for both the ecosystems and human communities, and understanding the impacts of environmental processes and human activities on ecosystems is of fundamental importance for the preservation of these services.

Recursions for the exchangeable partition function of the seedbank coalescent.

PubMed

Kurt, Noemi; Rafler, Mathias

2017-04-01

For the seedbank coalescent with mutation under the infinite alleles assumption, which describes the gene genealogy of a population with a strong seedbank effect subject to mutations, we study the distribution of the final partition with mutation. This generalizes the coalescent with freeze by Dong et al. (2007) to coalescents where ancestral lineages are blocked from coalescing. We derive an implicit recursion which we show to have a unique solution and give an interpretation in terms of absorption problems of a random walk. Moreover, we derive recursions for the distribution of the number of blocks in the final partition. Copyright © 2017 Elsevier Inc. All rights reserved.

Experimental demonstration of laser to x-ray conversion enhancements with low density gold targets

DOE PAGES

Shang, Wanli; Yang, Jiamin; Zhang, Wenhai; ...

2016-02-12

The enhancement of laser to x-ray conversion efficiencies using low density gold targets [W. L. Shang, J. M. Yang, and Y. S. Dong, Appl. Phys. Lett. 102, 094105 (2013)] is demonstrated. Laser to x-ray conversion efficiencies with 6.3% and 12% increases are achieved with target densities of 1 and 0.25 g/cm 3, when compared with that of a solid gold target (19.3 g/cm 3). Experimental data and numerical simulations are in good agreement. Lastly, the enhancement is caused by larger x-ray emission zone lengths formed in low density targets, which is in agreement with the simulation results.

Experimental demonstration of laser to x-ray conversion enhancements with low density gold targets

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shang, Wanli; Yang, Jiamin; Zhang, Wenhai

The enhancement of laser to x-ray conversion efficiencies using low density gold targets [W. L. Shang, J. M. Yang, and Y. S. Dong, Appl. Phys. Lett. 102, 094105 (2013)] is demonstrated. Laser to x-ray conversion efficiencies with 6.3% and 12% increases are achieved with target densities of 1 and 0.25 g/cm 3, when compared with that of a solid gold target (19.3 g/cm 3). Experimental data and numerical simulations are in good agreement. Lastly, the enhancement is caused by larger x-ray emission zone lengths formed in low density targets, which is in agreement with the simulation results.

Immunomodulation by adoptive regulatory T-cell transfer improves Coxsackievirus B3-induced myocarditis.

PubMed

Pappritz, Kathleen; Savvatis, Konstantinos; Miteva, Kapka; Kerim, Bahtiyar; Dong, Fengquan; Fechner, Henry; Müller, Irene; Brandt, Christine; Lopez, Begoña; González, Arantxa; Ravassa, Susana; Klingel, Karin; Diez, Javier; Reinke, Petra; Volk, Hans-Dieter; Van Linthout, Sophie; Tschöpe, Carsten

2018-06-04

Regulatory T (T reg ) cells offer new therapeutic options for controlling undesired systemic and local immune responses. The aim of the current study was to determine the impact of therapeutic T reg administration on systemic and cardiac inflammation and remodeling in coxsackievirus B3 (CVB3) -induced myocarditis. Therefore, syngeneic T reg cells were applied intravenously in CVB3-infected mice 3 d after infection. Compared with CVB3 + PBS mice, CVB3 + T reg mice exhibited lower left ventricular (LV) chemokine expression, accompanied by reduced cardiac presence of proinflammatory Ly6C high CCR2 high Cx3Cr1 low monocytes and higher retention of proinflammatory Ly6C mid CCR2 high Cx3Cr1 low monocytes in the spleen. In addition, splenic myelopoiesis was reduced in CVB3 + T reg compared with CVB3 + PBS mice. Coculture of T reg cells with splenocytes isolated from mice 3 d post-CVB3 infection further demonstrated the ability of T reg cells to modulate monocyte differentiation in favor of the anti-inflammatory Ly6C low CCR2 low Cx3Cr1 high subset. T reg -mediated immunomodulation was paralleled by lower collagen 1 protein expression and decreased levels of soluble and insoluble collagen in LV of CVB3 + T reg compared with CVB3 + PBS mice. In agreement with these findings, LV systolic and diastolic function was improved in CVB3 + T reg mice compared with CVB3 + PBS mice. In summary, adoptive T reg transfer in the inflammatory phase of viral-induced myocarditis protects the heart against inflammatory damage and fibrosis via modulation of monocyte subsets.-Pappritz, K., Savvatis, K., Miteva, K., Kerim, B., Dong, F., Fechner, H., Müller, I., Brandt, C., Lopez, B., González, A., Ravassa, S., Klingel, K., Diez, J., Reinke, P., Volk, H.-D., Van Linthout, S., Tschöpe, C. Immunomodulation by adoptive regulatory T-cell transfer improves Coxsackievirus B3-induced myocarditis.

Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran) in blood donors of south Gujarat, India

PubMed Central

Kahar, Manoj A.; Patel, Rajnikant. D.

2014-01-01

Background: This is the first study on phenotype frequencies of various blood group systems in blood donors of south Gujarat, India using conventional tube technique. Material and Methods: A total of 115 “O” blood group donors from three different blood banks of south Gujarat were typed for D, C, c, E, e, K, Jka, Lea, Leb, P1, M, and N antigens using monoclonal antisera and k, Kpa, Kpb, Fya,Fyb, Jkb, S,s, Lua, and Lub antigens were typed using polyclonal antisera employing Indirect Antiglobulin Test. Antigens and phenotype frequencies were expressed as percentages. Results: From the 115 blood donor samples used for extended antigen typing in the Rh system, e antigen was found in 100% donors, followed by D [84.35%], C [81.74%], c [56.32%], and E [21.74%] with DCe/DCe (R1 R1, 40.87%) as the most common phenotype. k was found to be positive in 100% of donors and no K+k- phenotype was found in Kell system. For Kidd and Duffy blood group system, Jk(a+b+) and Fy(a-b-) were the most common phenotypes with frequency of 52.17% and 48.69%, respectively. In the MNS system, 39.13% donors were typed as M+N+, 37.39% as M+N-, and 23.48% as M-N+. S+s+ was found in 24.35% of donors, S+s- in 8.69%, and S-s+ as the commonest amongst donors with 66.96%. No Lu(a+b+) or Lu(a+b-) phenotypes were detected in 115 donors typed for Lutheran antigens. A rare Lu(a-b-) phenotype was found in 2.61% donors. Conclusion: Data base for antigen frequency of various blood group systems in local donors help provide antigen negative compatible blood units to patients with multiple antibodies in order to formulate in-house red cells for antibody detection and identification and for preparing donor registry for rare blood groups. PMID:24678176

49 CFR 572.71 - General description.

Code of Federal Regulations, 2013 CFR

2013-10-01

... D SA 106C 041 Arm Assembly (right) SA 106C 001, sheet 14 A SA 106C 042 Arm Assembly (left) SA 106C... Assembly (left) SA 106C 001, sheet 17 A (c) Adjacent segments are joined in a manner such that except for...

49 CFR 572.71 - General description.

Code of Federal Regulations, 2011 CFR

2011-10-01

... D SA 106C 041 Arm Assembly (right) SA 106C 001, sheet 14 A SA 106C 042 Arm Assembly (left) SA 106C... Assembly (left) SA 106C 001, sheet 17 A (c) Adjacent segments are joined in a manner such that except for...

A Tyrosine-Based Trafficking Motif of the Tegument Protein pUL71 Is Crucial for Human Cytomegalovirus Secondary Envelopment.

PubMed

Dietz, Andrea N; Villinger, Clarissa; Becker, Stefan; Frick, Manfred; von Einem, Jens

2018-01-01

The human cytomegalovirus (HCMV) tegument protein pUL71 is required for efficient secondary envelopment and accumulates at the Golgi compartment-derived viral assembly complex (vAC) during infection. Analysis of various C-terminally truncated pUL71 proteins fused to enhanced green fluorescent protein (eGFP) identified amino acids 23 to 34 as important determinants for its Golgi complex localization. Sequence analysis and mutational verification revealed the presence of an N-terminal tyrosine-based trafficking motif (YXXΦ) in pUL71. This led us to hypothesize a requirement of the YXXΦ motif for the function of pUL71 in infection. Mutation of both the tyrosine residue and the entire YXXΦ motif resulted in an altered distribution of mutant pUL71 at the plasma membrane and in the cytoplasm during infection. Both YXXΦ mutant viruses exhibited similarly decreased focal growth and reduced virus yields in supernatants. Ultrastructurally, mutant-virus-infected cells exhibited impaired secondary envelopment manifested by accumulations of capsids undergoing an envelopment process. Additionally, clusters of capsid accumulations surrounding the vAC were observed, similar to the ultrastructural phenotype of a UL71-deficient mutant. The importance of endocytosis and thus the YXXΦ motif for targeting pUL71 to the Golgi complex was further demonstrated when clathrin-mediated endocytosis was inhibited either by coexpression of the C-terminal part of cellular AP180 (AP180-C) or by treatment with methyl-β-cyclodextrin. Both conditions resulted in a plasma membrane accumulation of pUL71. Altogether, these data reveal the presence of a functional N-terminal endocytosis motif that is an important determinant for intracellular localization of pUL71 and that is furthermore required for the function of pUL71 during secondary envelopment of HCMV capsids at the vAC. IMPORTANCE Human cytomegalovirus (HCMV) is the leading cause of birth defects among congenital virus infections and can lead to life-threatening infections in immunocompromised hosts. Current antiviral treatments target viral genome replication and are increasingly overcome by viral mutations. Therefore, identifying new targets for antiviral therapy is important for future development of novel treatment options. A detailed molecular understanding of the complex virus morphogenesis will identify potential viral as well as cellular targets for antiviral intervention. Secondary envelopment is an important viral process through which infectious virus particles are generated and which involves the action of several viral proteins, such as tegument protein pUL71. Targeting of pUL71 to the site of secondary envelopment appears to be crucial for its function during this process and is regulated by utilizing host trafficking mechanisms that are commonly exploited by viral glycoproteins. Thus, intracellular trafficking, if targeted, might present a novel target for antiviral therapy. Copyright © 2017 American Society for Microbiology.

Survival of Listeria monocytogenes in milk during high-temperature, short-time pasteurization.

PubMed Central

Doyle, M P; Glass, K A; Beery, J T; Garcia, G A; Pollard, D J; Schultz, R D

1987-01-01

Milk from cows inoculated with Listeria monocytogenes was pooled for 2 to 4 days and then heated at 71.7 to 73.9 degrees C for 16.4 s or at 76.4 to 77.8 degrees C for 15.4 s in a high-temperature, short-time plate heat exchanger pasteurization unit. L. monocytogenes was isolated from milk after heat treatment in six of nine pasteurization trials done at 71.7 to 73.9 degrees C and in none of three trials done at 76.4 to 77.8 degrees C. An average of 1.5 to 9.2 L. monocytogenes cells was seen in each milk polymorphonuclear leukocyte before heat treatment in 11 of 12 pasteurization trials. Noticeable degradation of leukocytes with intracellular listeria was detected in unpasteurized milk after 3 days of storage at 4 degrees C, and by 4 days of storage leukocytes had deteriorated to cellular debris, suggesting that holding unpasteurized milk refrigerated for 4 or more days would eliminate a protective effect leukocytes may provide for increasing heat resistance of L. monocytogenes. Results indicate that under the conditions of this study, L. monocytogenes can survive the minimum high-temperature, short-time treatment (71.7 degrees C, 15 s) required by the U.S. Food and Drug Administration for pasteurizing milk. Images PMID:3116926

Revealing charge carrier dynamics in squaraine:[6, 6]-phenyl-C 71-butyric acid methyl ester based organic solar cells

NASA Astrophysics Data System (ADS)

Rana, Aniket; Sharma, Chhavi; Prabhu, Deepak D.; Kumar, Mahesh; Karuvath, Yoosaf; Das, Suresh; Chand, Suresh; Singh, Rajiv K.

2018-04-01

Ultrafast charge carrier dynamics as well as the generation of polaron pair in squaraine (SQ) and squaraine:[6,6]-phenyl-C 71-butyric acid methyl ester (SQ:PCBM71) have been studied using ultrafast transient absorption spectroscopy (UTAS). The current study reveals that the pure SQ exhibits the creation of singlet and triplet states; however, incorporation of PCBM71 in SQ results in the formation of polaron pairs with ˜550ps lifetime, which in turn leads to the creation of free electrons in the device. We show that the considerable increment in monomolecular and bimolecular recombination in SQ:PCBM71 compared to pure SQ which describes the interfacial compatibility of SQ and PCBMC71 molecules. The present work not only provides the information about the carrier generation in SQ and SQ:PCBM71 but also gives the facts relating to the effect of PCBM71 mixing into the SQ which is very significant because the SQ has donor-acceptor-donor (D-A-D) structure and mixing one more acceptor can introduce more complex recombinations in the blend. These findings have been complimented by the charge transport study in the device using impedance spectroscopy. The various important transport parameters are transit time (τt), diffusion constant (Dn), global mobility (μ) and carrier lifetime (τr). The values of these parameters are 26.38 μs, 4.64x10-6 cm2s-1, 6.12x10-6 cm2V-1s-1 and 399 μs, respectively. To the best of our knowledge such study related to SQ is not present in the literature comprehensively.

[The genetical evolution of the full length genes of 5 EV 71 strains from 5 Shenzhen patients with hand-food-mouth disease associated with EV71 infection].

PubMed

Liu, Wei-long; Yang, Gui-lin; Wei, Qing; Zhang, Ming-xia; Chen, Xin-chun; Liu, Ying-xia; Gao, Yang; Zhou, Bo-ping

2011-02-01

To investigate the characteristics of molecular epidemiology and molecular evolution of 5 EV 71 (enterovirus 71, EV71) strains from 5 Shenzhen patients with hand-food-mouth disease associated with EV 71 infection. 5 EV 71 strains were isolated, and sequenced to analyzed the full length gene sequences in order to compare nucleotide and amino acid homology with other EV71 strains from other regions and countries as well as previous strains across the world through bioinformatics software. 5 strains of EV 71 belonged to sub-genotype C4 by analysis of nucleotide sequences of VP1 and VP4 of EV 71. The differences of nucleotide and amino acid sequences were much small with nucleotide homology of 93% and amino acid homology of 98% among these 5 strains. A phylogenetic tree analysis indicated that 2008 Shenzhen epidemic strains were the most close to 2004 Shenzhen circulating strains, and also much close to 1998 Shenzhen epidemic strains and 2008 Fuyang Anhui strains. The dead strain was very close to 2008 Fuyang Anhui epidemic strains. It can be speculated that this epidemic strains of EV 71 probably originate from the same ancient strain in the history, may from 1998 Shenzhen strain.

10 CFR 71.101 - Quality assurance requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... quality assurance actions related to control of the physical characteristics and quality of the material... through 71.137 and satisfying any specific provisions that are applicable to the licensee's activities... assurance requirement's importance to safety. (c) Approval of program. (1) Before the use of any package for...

40 CFR 464.21 - Specialized definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

.... pentachlorophenol 66. bis(2-ethylhexyl)phthalate 71. dimethyl phthalate (2) Dust Collection Scrubbers (§ 464.25(c...-dimethylphenol 55. naphthalene 58. 4-nitrophenol 64. pentachlorophenol 65. phenol 66. bis(2-ethylhexyl)phthalate 67. butyl benzyl phthalate 68. di-n-butyl phthalate 70. diethyl phthalate 71. dimethyl phthalate 72...

40 CFR 464.21 - Specialized definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

.... pentachlorophenol 66. bis(2-ethylhexyl)phthalate 71. dimethyl phthalate (2) Dust Collection Scrubbers (§ 464.25(c...-dimethylphenol 55. naphthalene 58. 4-nitrophenol 64. pentachlorophenol 65. phenol 66. bis(2-ethylhexyl)phthalate 67. butyl benzyl phthalate 68. di-n-butyl phthalate 70. diethyl phthalate 71. dimethyl phthalate 72...

10 CFR 71.125 - Control of measuring and test equipment.

Code of Federal Regulations, 2010 CFR

2010-01-01

... MATERIAL Quality Assurance § 71.125 Control of measuring and test equipment. The licensee, certificate holder, and applicant for a CoC shall establish measures to assure that tools, gauges, instruments, and other measuring and testing devices used in activities affecting quality are properly controlled...

Performance Evaluation at the Hardware Architecture Level and the Operating System Kernel Design Level.

DTIC Science & Technology

1977-12-01

Rev’ieuw c:, P i. I.I!i Work 6 5.3. GC!.’crir~i’w :.f t -(c- Experimcantai Setup .65 5.4. 1 ocls it- C -nip Hy)dra) environment 67 5.5. The mc-.c’ 71 ...to segments 2 Vammnce due to programs crA Var-mance dUe to application areas. I h . i .,,I1111 al v I- timnaied wow"~ the mcr .(I ii ’ Iw irw I( (lIn...1,030 .. .311 I 1 -011 111,) .1101 131 0.0..) .11, 4 C NIlF .::’) . 12A IB, l) a liB. r] .4 o, , 5 M. OS148 813 050.3 71 ,).[3 F’"* .1:. 0(0,0] 0 0] S 14

Expression of Kir7.1 and a Novel Kir7.1 Splice Variant in Native Human Retinal Pigment Epithelium

PubMed Central

Yang, Dongli; Swaminathan, Anuradha; Zhang, Xiaoming; Hughes, Bret A.

2009-01-01

Previous studies on bovine retinal pigment epithelium (RPE) established that Kir7.1 channels compose this epithelium’s large apical membrane K+ conductance. The purpose of this study was to determine whether Kir7.1 and potential Kir7.1 splice variants are expressed in native adult human RPE and, if so, to determine their function and how they are generated. RT-PCR analysis indicated that human RPE expresses full-length Kir7.1 and a novel Kir7.1 splice variant, designated Kir7.1S. Analysis of the human Kir7.1 gene (KCNJ13) organization revealed that it contains 3 exons, 2 introns, and a novel alternative 5′ splice site in exon 2. In human RPE, the alternative usage of two competing 5′ splice sites in exon 2 gives rise to transcripts encoding full-length Kir7.1 and Kir7.1S, which is predicted to encode a truncated protein. Real-time PCR indicated that Kir7.1 transcript is nearly as abundant as GAPDH mRNA in human RPE whereas Kir7.1S transcript expression is 4-fold lower. Western blot analysis showed that the splice variant is translated in Xenopus oocytes injected with Kir7.1S cRNA and revealed the expression of full-length Kir7.1 but not Kir7.1S in adult human RPE. Co-expression of Kir7.1 with Kir7.1S in Xenopus oocytes had no effect on either the kinetics or amplitude of Kir7.1 currents. This study confirms the expression of Kir7.1 in human RPE, identifies a Kir7.1 splice variant resulting in predicted changes in protein sequence, and indicates that there no functional interaction between this splice variant and full-length Kir7.1. PMID:18035352

Proton irradiation study of GFR candidate ceramics

NASA Astrophysics Data System (ADS)

Gan, Jian; Yang, Yong; Dickson, Clayton; Allen, Todd

2009-06-01

This work investigated the microstructural response of SiC, ZrC and ZrN irradiated with 2.6 MeV protons at 800 °C to a fluence of 2.75 × 10 19 protons/cm 2, corresponding to 0.71-1.8 displacement per atom (dpa), depending on the material. The change of lattice constant evaluated using HOLZ patterns is not observed. In comparison to Kr ion irradiation at 800 °C to 10 dpa from the previous studies, the proton irradiated ZrC and ZrN at 1.8 dpa show less irradiation damage to the lattice structure. The proton irradiated ZrC exhibits faulted loops which are not observed in the Kr ion irradiated sample. ZrN shows the least microstructural change from proton irradiation. The microstructure of 6H-SiC irradiated to 0.71 dpa consists of black dot defects at high density.

Diketopyrrolopyrrole-based polymer:fullerene nanoparticle films with thermally stable morphology for organic photovoltaic applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Holmes, Natalie P.; Vaughan, Ben; Williams, Evan L.

Polymer:fullerene nanoparticles (NPs) offer two key advantages over bulk heterojunction (BHJ) films for organic photovoltaics (OPVs), water-processability and potentially superior morphological control. Once an optimal active layer morphology is reached, maintaining this morphology at OPV operating temperatures is key to the lifetime of a device. Here in this paper we study the morphology of the PDPP-TNT (poly{3,6-dithiophene-2-yl-2,5-di(2-octyldodecyl)-pyrrolo[3,4-c]pyrrole-1,4-dione-alt-naphthalene}):PC 71BM ([6,6]-phenyl C 71 butyric acid methyl ester) NP system and then compare the thermal stability of NP and BHJ films to the common poly(3-hexylthiophene) (P3HT): phenyl C 61 butyric acid methyl ester (PC 61BM) system. We find that material T g playsmore » a key role in the superior thermal stability of the PDPP-TNT:PC 71BM system; whereas for the P3HT:PC 61BM system, domain structure is critical.« less

Detection of human enterovirus 71 and coxsackievirus A16 in children with hand, foot and mouth disease in China.

PubMed

Chen, Ling; Mou, Xiaozhou; Zhang, Qiong; Li, Yifei; Lin, Jian; Liu, Fanlong; Yuan, Li; Tang, Yiming; Xiang, Charlie

2012-04-01

The aims of the present study were to investigate the genetic characteristics of enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) strains in China and to evaluate the relationship between the genotypes of CVA16 and EV71 and their geographical distribution. A total of 399 stool specimens were collected from children with symptoms of hand, foot and mouth disease (HFMD) in Zhejiang Province. The presence of enteroviruses was determined using reverse transcription-semi-nested PCR targeted to the VP1 gene of all human enteroviruses and DNA sequencing. EV71 and CVA16, the major etiological agents of HFMD, were detected in 38.4% (38/99) and 35.4% (35/99) of HEV-A species-positive cases, respectively. Based on the phylogenetic analysis of the VP1 gene, EV71 strains identified in this study belong to subgenotype C4, and CVA16 strains herein were classified into clusters B2a and B2b within the genotype B2. Taking into consideration other published data, we conclude that the genetic characteristics of enteroviruses in China reflect the pattern of the endemic circulation of the subgenotype C4 to EV71 and clusters B2a and B2b within genotype B2 to CVA16, which have been continuously circulating in China since 1997. This observation indicates that the genetic characteristics of enteroviruses in China seem to depend on their special geographical and climatical features allowing them to be sustained with little external effect.

Detection of human enterovirus 71 and coxsackievirus A16 in children with hand, foot and mouth disease in China

PubMed Central

CHEN, LING; MOU, XIAOZHOU; ZHANG, QIONG; LI, YIFEI; LIN, JIAN; LIU, FANLONG; YUAN, LI; TANG, YIMING; XIANG, CHARLIE

2012-01-01

The aims of the present study were to investigate the genetic characteristics of enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) strains in China and to evaluate the relationship between the genotypes of CVA16 and EV71 and their geographical distribution. A total of 399 stool specimens were collected from children with symptoms of hand, foot and mouth disease (HFMD) in Zhejiang Province. The presence of enteroviruses was determined using reverse transcription-semi-nested PCR targeted to the VP1 gene of all human enteroviruses and DNA sequencing. EV71 and CVA16, the major etiological agents of HFMD, were detected in 38.4% (38/99) and 35.4% (35/99) of HEV-A species-positive cases, respectively. Based on the phylogenetic analysis of the VP1 gene, EV71 strains identified in this study belong to subgenotype C4, and CVA16 strains herein were classified into clusters B2a and B2b within the genotype B2. Taking into consideration other published data, we conclude that the genetic characteristics of enteroviruses in China reflect the pattern of the endemic circulation of the subgenotype C4 to EV71 and clusters B2a and B2b within genotype B2 to CVA16, which have been continuously circulating in China since 1997. This observation indicates that the genetic characteristics of enteroviruses in China seem to depend on their special geographical and climatical features allowing them to be sustained with little external effect. PMID:22218731

Inhibition of EV71 by curcumin in intestinal epithelial cells.

PubMed

Huang, Hsing-I; Chio, Chi-Chong; Lin, Jhao-Yin

2018-01-01

EV71 is a positive-sense single-stranded RNA virus that belongs to the Picornaviridae family. EV71 infection may cause various symptoms ranging from hand-foot-and-mouth disease to neurological pathological conditions such as aseptic meningitis, ataxia, and acute transverse myelitis. There is currently no effective treatment or vaccine available. Various compounds have been examined for their ability to restrict EV71 replication. However, most experiments have been performed in rhabdomyosarcoma or Vero cells. Since the gastrointestinal tract is the entry site for this pathogen, we anticipated that orally ingested agents may exert beneficial effects by decreasing virus replication in intestinal epithelial cells. In this study, curcumin (diferuloylmethane, C21H20O6), an active ingredient of turmeric (Curcuma longa Linn) with anti-cancer properties, was investigated for its anti-enterovirus activity. We demonstrate that curcumin treatment inhibits viral translation and increases host cell viability. Curcumin does not exert its anti-EV71 effects by modulating virus attachment or virus internal ribosome entry site (IRES) activity. Furthermore, curcumin-mediated regulation of mitogen-activated protein kinase (MAPK) signaling pathways is not involved. We found that protein kinase C delta (PKCδ) plays a role in virus translation in EV71-infected intestinal epithelial cells and that curcumin treatment decreases the phosphorylation of this enzyme. In addition, we show evidence that curcumin also limits viral translation in differentiated human intestinal epithelial cells. In summary, our data demonstrate the anti-EV71 properties of curcumin, suggesting that ingestion of this phytochemical may protect against enteroviral infections.

Inhibition of EV71 by curcumin in intestinal epithelial cells

PubMed Central

Chio, Chi-Chong; Lin, Jhao-Yin

2018-01-01

EV71 is a positive-sense single-stranded RNA virus that belongs to the Picornaviridae family. EV71 infection may cause various symptoms ranging from hand-foot-and-mouth disease to neurological pathological conditions such as aseptic meningitis, ataxia, and acute transverse myelitis. There is currently no effective treatment or vaccine available. Various compounds have been examined for their ability to restrict EV71 replication. However, most experiments have been performed in rhabdomyosarcoma or Vero cells. Since the gastrointestinal tract is the entry site for this pathogen, we anticipated that orally ingested agents may exert beneficial effects by decreasing virus replication in intestinal epithelial cells. In this study, curcumin (diferuloylmethane, C21H20O6), an active ingredient of turmeric (Curcuma longa Linn) with anti-cancer properties, was investigated for its anti-enterovirus activity. We demonstrate that curcumin treatment inhibits viral translation and increases host cell viability. Curcumin does not exert its anti-EV71 effects by modulating virus attachment or virus internal ribosome entry site (IRES) activity. Furthermore, curcumin-mediated regulation of mitogen-activated protein kinase (MAPK) signaling pathways is not involved. We found that protein kinase C delta (PKCδ) plays a role in virus translation in EV71-infected intestinal epithelial cells and that curcumin treatment decreases the phosphorylation of this enzyme. In addition, we show evidence that curcumin also limits viral translation in differentiated human intestinal epithelial cells. In summary, our data demonstrate the anti-EV71 properties of curcumin, suggesting that ingestion of this phytochemical may protect against enteroviral infections. PMID:29370243

Pilot scale production of highly efficacious and stable enterovirus 71 vaccine candidates.

PubMed

Chou, Ai-Hsiang; Liu, Chia-Chyi; Chang, Cheng-Peng; Guo, Meng-Shin; Hsieh, Shih-Yang; Yang, Wen-Hsueh; Chao, Hsin-Ju; Wu, Chien-Long; Huang, Ju-Lan; Lee, Min-Shi; Hu, Alan Yung-Chi; Lin, Sue-Chen; Huang, Yu-Yun; Hu, Mei-Hua; Chow, Yen-Hung; Chiang, Jen-Ron; Chang, Jui-Yuan; Chong, Pele

2012-01-01

Enterovirus 71 (EV71) has caused several epidemics of hand, foot and mouth diseases (HFMD) in Asia and now is being recognized as an important neurotropic virus. Effective medications and prophylactic vaccine against EV71 infection are urgently needed. Based on the success of inactivated poliovirus vaccine, a prototype chemically inactivated EV71 vaccine candidate has been developed and currently in human phase 1 clinical trial. In this report, we present the development of a serum-free cell-based EV71 vaccine. The optimization at each step of the manufacturing process was investigated, characterized and quantified. In the up-stream process development, different commercially available cell culture media either containing serum or serum-free was screened for cell growth and virus yield using the roller-bottle technology. VP-SFM serum-free medium was selected based on the Vero cell growth profile and EV71 virus production. After the up-stream processes (virus harvest, diafiltration and concentration), a combination of gel-filtration liquid chromatography and/or sucrose-gradient ultracentrifugation down-stream purification processes were investigated at a pilot scale of 40 liters each. Although the combination of chromatography and sucrose-gradient ultracentrifugation produced extremely pure EV71 infectious virus particles, the overall yield of vaccine was 7-10% as determined by a VP2-based quantitative ELISA. Using chromatography as the downstream purification, the virus yield was 30-43%. To retain the integrity of virus neutralization epitopes and the stability of the vaccine product, the best virus inactivation was found to be 0.025% formalin-treatment at 37 °C for 3 to 6 days. Furthermore, the formalin-inactivated virion vaccine candidate was found to be stable for >18 months at 4 °C and a microgram of viral proteins formulated with alum adjuvant could induce strong virus-neutralizing antibody responses in mice, rats, rabbits, and non-human primates. These results provide valuable information supporting the current cell-based serum-free EV71 vaccine candidate going into human Phase I clinical trials.

Simulating Radar Signals for Detection Performance Evaluation.

DTIC Science & Technology

1981-02-01

defined by g = e-(aO/e 3dB) 2 (70) where a = 1.6651 and 0 3dB Is the one-way half-power heamwidth, we note that from (60) and (68) a=( 03dB) 2 , ( 71 ...8217erform;ice of Some Nonparametric Rank Tests and an Alpplication to Radar," JEEE ’’ra.. Information Theory , Vol. IT-16, pp 309- 318 (c!;pecjally Section...V1 1 1 C . fl I V IC ’A It N FI (.LLTrif -I~d P’LE (,%I I CiRCS-SE CT IC& AREA C vu I$1 i rKI I %1 : 111. C I -PjI Ik ~I G%ft- 71 -Y: I I LAl. I J10F

Hybrid photodetector based on CsPbBr3 perovskite nanocrystals and PC71BM fullerene derivative

NASA Astrophysics Data System (ADS)

Li, Tengteng; Liu, Meihong; Li, Qingyan; Chen, Run; Liu, Xin

2018-05-01

Here, a hybrid perovskite-organic photodetector was fabricated by integrating CsPbBr3 nanocrystals (NCs) with [6,6]-phenyl C71 butyric acid methyl ester (PC71BM), exhibiting remarkable optoelectronic properties in terms of photoresponsivity (1.72 A/W), detectivity (1.76 × 107 Jones), external quantum efficiency (EQE) (530%) under the illumination of 405 nm laser, and photoresponse time is shorter than 0.1 ms due to the assistance of heterojunction on the separation of photoexcitons.

Fate of Escherichia coli O157:H7 in mechanically tenderized beef prime rib following searing, cooking, and holding under commercial conditions.

PubMed

Porto-Fett, Anna C S; Shoyer, Bradley A; Thippareddi, Harshavardhan; Luchansky, John B

2013-03-01

We evaluated the effect of commercial times and temperatures for searing, cooking, and holding on the destruction of Escherichia coli O157:H7 (ECOH) within mechanically tenderized prime rib. Boneless beef ribeye was inoculated on the fat side with ca. 5.7 log CFU/g of a five-strain cocktail of ECOH and then passed once through a mechanical tenderizer with the fat side facing upward. The inoculated and tenderized prime rib was seared by broiling at 260°C for 15 min in a conventional oven and then cooked in a commercial convection oven at 121.1°C to internal temperatures of 37.8, 48.9, 60.0, and 71.1°C before being placed in a commercial holding oven maintained at 60.0°C for up to 8 h. After searing, ECOH levels decreased by ca. 1.0 log CFU/g. Following cooking to internal temperatures of 37.8 to 71.1°C, pathogen levels decreased by an additional ca. 2.7 to 4.0 log CFU/g. After cooking to 37.8, 48.9, or 60.0°C and then warm holding at 60.0°C for 2 h, pathogen levels increased by ca. 0.2 to 0.7 log CFU/g. However, for prime rib cooked to 37.8°C, pathogen levels remained relatively unchanged over the next 6 h of warm holding, whereas for those cooked to 48.9 or 60.0°C pathogen levels decreased by ca. 0.3 to 0.7 log CFU/g over the next 6 h of warm holding. In contrast, after cooking prime rib to 71.1°C and holding for up to 8 h at 60.0°C, ECOH levels decreased by an additional ca. 0.5 log CFU/g. Our results demonstrated that to achieve a 5.0-log reduction of ECOH in blade tenderized prime rib, it would be necessary to sear at 260°C for 15 min, cook prime rib to internal temperatures of 48.9, 60.0, or 71.1°C, and then hold at 60.0°C for at least 8 h.

Susceptibility baseline and chlorantraniliprole resistance monitoring in Chilo suppressalis (Lepidoptera: Pyralidae).

PubMed

Gao, Congfen; Yao, Rong; Zhang, Zhenzhen; Wu, Min; Zhang, Shuai; Su, Jianya

2013-10-01

Chlorantraniliprole, a new anthranilic diamide insecticide, has been commercialized in China since 2008 for controlling of several lepidopterans, including rice stem borer, Chilo suppressalis Walker. Chemical control of this pest has become difficult because of its resistance development to many conventional insecticides. To facilitate chlorantraniliprole-resistance monitoring, seedling dip bioassays were conducted in 2011 and 2012 to assess the susceptibility of 30 populations of C. suppressalis from seven provinces in China. The assays established a larval susceptibility baseline with LC50 at 1.393 mg a.i/liter. The toxicity (LC50) of chlorantraniliprole against second-instar larvae of field populations ranged from 0.568 (SL12) to 13.547 (RA12) mg a.i/liter. Substantial variations of the susceptibility to chlorantraniliprole were detected among different geographic populations, but no significant difference was observed between years for most populations except for populations from Dong'an, Cangnan, and Lujiang. Resistance ratios to the chemical ranged from 1.0 to 9.7, indicating that most colonies remained susceptible or showed certain decrease in susceptibility. Approximately, 13.3% of the populations exhibited low levels of resistance to chlorantraniliprole. These data are useful in future monitoring program for detecting any changes in susceptibility as a result of use of the insecticide.

FGF21 is induced in cisplatin nephrotoxicity to protect against kidney tubular cell injury.

PubMed

Li, Fanghua; Liu, Zhiwen; Tang, Chengyuan; Cai, Juan; Dong, Zheng

2018-01-22

Cisplatin, a widely used cancer therapy drug, induces nephrotoxicity or acute kidney injury (AKI), but the underlying mechanism remains unclear, and renal protective approaches are not available. Fibroblast growth factor (FGF)21 is an endocrine factor that regulates glucose uptake, metabolism, and energy expenditure. However, recent work has also implicated FGF21 in cellular stress response under pathogenic conditions. The role and regulation of FGF21 in AKI are unclear. Here, we show that FGF21 was dramatically induced during cisplatin treatment of renal tubular cells in vitro and mouse kidneys in vivo. The inductive response was suppressed by pifithrin (a pharmacological inhibitor of P53), suggesting a role of P53 in FGF21 induction. In cultured renal tubular cells, knockdown of FGF21 aggravated cisplatin-induced apoptosis, whereas supplementation of recombinant FGF21 was protective. Consistently, recombinant FGF21 alleviated cisplatin-induced kidney dysfunction, tissue damage, and tubular apoptosis in mice. Mechanistically, FGF21 suppressed P53 induction and activation during cisplatin treatment. Together, these results indicate that FGF21 is induced during cisplatin nephrotoxicity to protect renal tubules, and recombinant FGF21 may have therapeutic potential.-Li, F., Liu, Z., Tang, C., Cai, J., Dong, Z. FGF21 is induced in cisplatin nephrotoxicity to protect against kidney tubular cell injury.

Pusan East AFS K-9, Pusan, Korea. Revised Uniform Summary of Surface Weather Observations (RUSSWO)

DTIC Science & Technology

1968-02-26

ww.5 .5 - . . - --- "_ __ WNW * f_ 1*W 6.0 1W*__ NNIW .4 o f I 2_ es ofŕ’ fog__ __ v__. -’ ._ .I-_- __ - _ _ 11 -"- -- CALM 2.9. I Iso $ 127.0 2.3 01...27.2 48.3 48.9 49.6 49.6 9.6 49.7 49. 9.7 7T.7 4 ;9.7 49-/ 149. .7 ? iso 130.5 56.8 59.4 60.5 60.5 60.5 60.6 60.6 60.6 60.6 6C.6 6C.6 6C.6 6C.6 6C.6 cC...72.5 72. 72 . 72.5 72: I r Boo 26.7 68.3 70.3 71.6 73.0 73.1 73.6 73.7 73.7 74.C0 74.0 74. ,C 407. 27000 26.7 68.6 710.6 71.8 73.3 73.4 73.9 14.0

Geographic List of Prime Contract Awards. Oct 91 - Sep 92. FY92. (Naval Base Washington - Greenland)

DTIC Science & Technology

1993-01-01

000 0 c3 -- q 4 inionO 00 Ocl in MEM < CO (71) 11 c’) cl) 0) on oil I < c> M (71 l" r., r- 1- 11 r-- 0 10 Q Cý c> Q c> 0 (01010 Coo c’) 10 c"I...0)0)))000)a)0M ) 41I1 I0ON1 It10. Dr if I 0)oN 1100.. .l > OHp I0ON 14 0f 0 0 0. Mi 0ON 110 < -0 - 0 I-t0 1I03 0ON 110 P-4 0 - 2f) : M0ON 1100 00: W...40D 0 0 me .-404H-4 0s NIV O IN i 0 --4 C me I 0-4- )I t mem (-, 0100) * o- a0 Z 0 ( 1 00-o4 If Z >-Ie On o m4 4)I( 004c4 Is -HOW 0( in -# 00.- it rZ

Fate of Shiga toxin-producing O157:H7 and non-O157:H7 Escherichia coli cells within refrigerated, frozen, or frozen then thawed ground beef patties cooked on a commercial open-flame gas or a clamshell electric grill.

PubMed

Luchansky, John B; Porto-Fett, Anna C S; Shoyer, Bradley A; Phillips, John; Chen, Vivian; Eblen, Denise R; Cook, L Victor; Mohr, Tim B; Esteban, Emilio; Bauer, Nathan

2013-09-01

Both high-fat and low-fat ground beef (percent lean:fat = ca. 70:30 and 93:7, respectively) were inoculated with a 6-strain cocktail of non-O157:H7 Shiga toxin-producing Escherichia coli (STEC) or a five-strain cocktail of E. coli O157:H7 (ca. 7.0 log CFU/g). Patties were pressed (ca. 2.54 cm thick, ca. 300 g each) and then refrigerated (4°C, 18 to 24 h), or frozen (-18°C, 3 weeks), or frozen (-18°C, 3 weeks) and then thawed (4°C for 18 h or 21°C for 10 h) before being cooked on commercial gas or electric grills to internal temperatures of 60 to 76.6°C. For E. coli O157:H7, regardless of grill type or fat level, cooking refrigerated patties to 71.1 or 76.6°C decreased E. coli O157:H7 numbers from an initial level of ca. 7.0 log CFU/g to a final level of ≤1.0 log CFU/g, whereas decreases to ca. 1.1 to 3.1 log CFU/g were observed when refrigerated patties were cooked to 60.0 or 65.5°C. For patties that were frozen or freeze-thawed and cooked to 71.1 or 76.6°C, E. coli O157:H7 numbers decreased to ca. 1.7 or ≤0.7 log CFU/g. Likewise, pathogen numbers decreased to ca. 0.7 to 3.7 log CFU/g in patties that were frozen or freeze-thawed and cooked to 60.0 or 65.5°C. For STEC, regardless of grill type or fat level, cooking refrigerated patties to 71.1 or 76.6°C decreased pathogen numbers from ca. 7.0 to ≤0.7 log CFU/g, whereas decreases to ca. 0.7 to 3.6 log CFU/g were observed when refrigerated patties were cooked to 60.0 or 65.5°C. For patties that were frozen or freeze-thawed and cooked to 71.1 or 76.6°C, STEC numbers decreased to a final level of ca. 1.5 to ≤0.7 log CFU/g. Likewise, pathogen numbers decreased from ca. 7.0 to ca. 0.8 to 4.3 log CFU/g in patties that were frozen or freeze-thawed and cooked to 60.0 or 65.5°C. Thus, cooking ground beef patties that were refrigerated, frozen, or freeze-thawed to internal temperatures of 71.1 and 76.6°C was effective for eliminating ca. 5.1 to 7.0 log CFU of E. coli O157:H7 and STEC per g.

Treatment of osteomyelitis defects by a vancomycin-loaded gelatin/β-tricalcium phosphate composite scaffold

PubMed Central

Zhou, J.; Zhou, X. G.; Wang, J. W.; Zhou, H.; Dong, J.

2018-01-01

Objective In the present study, we aimed to assess whether gelatin/β-tricalcium phosphate (β-TCP) composite porous scaffolds could be used as a local controlled release system for vancomycin. We also investigated the efficiency of the scaffolds in eliminating infections and repairing osteomyelitis defects in rabbits. Methods The gelatin scaffolds containing differing amounts of of β-TCP (0%, 10%, 30% and 50%) were prepared for controlled release of vancomycin and were labelled G-TCP0, G-TCP1, G-TCP3 and G-TCP5, respectively. The Kirby-Bauer method was used to examine the release profile. Chronic osteomyelitis models of rabbits were established. After thorough debridement, the osteomyelitis defects were implanted with the scaffolds. Radiographs and histological examinations were carried out to investigate the efficiency of eliminating infections and repairing bone defects. Results The prepared gelatin/β-TCP scaffolds exhibited a homogeneously interconnected 3D porous structure. The G-TCP0 scaffold exhibited the longest duration of vancomycin release with a release duration of eight weeks. With the increase of β-TCP contents, the release duration of the β-TCP-containing composite scaffolds was decreased. The complete release of vancomycin from the G-TCP5 scaffold was achieved within three weeks. In the treatment of osteomyelitis defects in rabbits, the G-TCP3 scaffold showed the most efficacious performance in eliminating infections and repairing bone defects. Conclusions The composite scaffolds could achieve local therapeutic drug levels over an extended duration. The G-TCP3 scaffold possessed the optimal porosity, interconnection and controlled release performance. Therefore, this scaffold could potentially be used in the treatment of chronic osteomyelitis defects. Cite this article: J. Zhou, X. G. Zhou, J. W. Wang, H. Zhou, J. Dong. Treatment of osteomyelitis defects by a vancomycin-loaded gelatin/β-tricalcium phosphate composite scaffold. Bone Joint Res 2018;7:46–57. DOI: 10.1302/2046-3758.71.BJR-2017-0129.R2. PMID:29330343

WATER IN COMETS 71P/CLARK AND C/2004 B1 (LINEAR) WITH SPITZER

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bockelee-Morvan, Dominique; Woodward, Charles E.; Kelley, Michael S.

2009-05-10

We present 5.5-7.6 {mu}m spectra of comets 71P/Clark (2006 May 27.56 UT, r{sub h} = 1.57 AU pre-perihelion) and C/2004 B1 (LINEAR) (2005 October 15.22 UT, r{sub h} = 2.21 AU pre-perihelion and 2006 May 16.22 UT, r{sub h} = 2.06 AU post-perihelion) obtained with the Spitzer Space Telescope. The {nu}{sub 2} vibrational band of water is detected with a signal-to-noise ratio of 11-50. Fitting the spectra using a fluorescence model of water emission yields a water rotational temperature of < 18 K for 71P/Clark and {approx_equal}14 {+-} 2 K (pre-perihelion) and 23 {+-} 4 K (post-perihelion) for C/2004 B1more » (LINEAR). The water ortho-to-para ratio in C/2004 B1 (LINEAR) is measured to be 2.31 {+-} 0.18, which corresponds to a spin temperature of 26{sup +3} {sub -2} K. Water production rates are derived. The agreement between the water model and the measurements is good, as previously found for Spitzer spectra of C/2003 K4 (LINEAR). The Spitzer spectra of these three comets do not show any evidence for emission from polycyclic aromatic hydrocarbons and carbonate minerals, in contrast to results reported for comets 9P/Tempel 1 and C/1995 O1 (Hale-Bopp)« less

32 CFR 842.71 - Delegations of authority.

Code of Federal Regulations, 2013 CFR

2013-07-01

... National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE CLAIMS AND LITIGATION ADMINISTRATIVE CLAIMS International Agreement Claims (10 U.S.C. 2734a and 2734b) § 842.71 Delegations of... pay a pro rata share of a claim or object to a claim in any amount: (1) The Secretary of the Air Force...

32 CFR 842.71 - Delegations of authority.

Code of Federal Regulations, 2012 CFR

2012-07-01

... National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE CLAIMS AND LITIGATION ADMINISTRATIVE CLAIMS International Agreement Claims (10 U.S.C. 2734a and 2734b) § 842.71 Delegations of... pay a pro rata share of a claim or object to a claim in any amount: (1) The Secretary of the Air Force...

32 CFR 842.71 - Delegations of authority.

Code of Federal Regulations, 2014 CFR

2014-07-01

... National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE CLAIMS AND LITIGATION ADMINISTRATIVE CLAIMS International Agreement Claims (10 U.S.C. 2734a and 2734b) § 842.71 Delegations of... pay a pro rata share of a claim or object to a claim in any amount: (1) The Secretary of the Air Force...

9 CFR 71.19 - Identification of swine in interstate commerce.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Identification of swine in interstate... GENERAL PROVISIONS § 71.19 Identification of swine in interstate commerce. (a)(1) Except as provided in paragraphs (c) and (g) of this section, no swine may be sold, transported, received for transportation, or...

9 CFR 71.19 - Identification of swine in interstate commerce.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Identification of swine in interstate... GENERAL PROVISIONS § 71.19 Identification of swine in interstate commerce. (a)(1) Except as provided in paragraphs (c) and (g) of this section, no swine may be sold, transported, received for transportation, or...

9 CFR 71.19 - Identification of swine in interstate commerce.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Identification of swine in interstate... GENERAL PROVISIONS § 71.19 Identification of swine in interstate commerce. (a)(1) Except as provided in paragraphs (c) and (g) of this section, no swine may be sold, transported, received for transportation, or...

9 CFR 71.19 - Identification of swine in interstate commerce.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Identification of swine in interstate... GENERAL PROVISIONS § 71.19 Identification of swine in interstate commerce. (a)(1) Except as provided in paragraphs (c) and (g) of this section, no swine may be sold, transported, received for transportation, or...

Automated UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 and selection for microaerophilic growth and ethanol production at elevated temperature on biomass sugars.

PubMed

Hughes, Stephen R; Bang, Sookie S; Cox, Elby J; Schoepke, Andrew; Ochwat, Kate; Pinkelman, Rebecca; Nelson, Danielle; Qureshi, Nasib; Gibbons, William R; Kurtzman, Cletus P; Bischoff, Kenneth M; Liu, Siqing; Cote, Gregory L; Rich, Joseph O; Jones, Marjorie A; Cedeño, David; Doran-Peterson, Joy; Riaño-Herrera, Nestor M; Rodríguez-Valencia, Nelson; López-Núñez, Juan C

2013-08-01

The yeast Kluyveromyces marxianus is a potential microbial catalyst for fuel ethanol production from a wide range of biomass substrates. To improve its growth and ethanol yield at elevated temperature under microaerophilic conditions, K. marxianus NRRL Y-1109 was irradiated with UV-C using automated protocols on a robotic platform for picking and spreading irradiated cultures and for processing the resulting plates. The plates were incubated under anaerobic conditions on xylose or glucose for 5 mo at 46 °C. Two K. marxianus mutant strains (designated 7-1 and 8-1) survived and were isolated from the glucose plates. Both mutant strains, but not wild type, grew aerobically on glucose at 47 °C. All strains grew anaerobically at 46 °C on glucose, galactose, galacturonic acid, and pectin; however, only 7-1 grew anaerobically on xylose at 46 °C. Saccharomyces cerevisiae NRRL Y-2403 did not grow at 46 °C on any of these substrates. With glucose as a carbon source, ethanol yield after 3 d at 46 °C was higher for 8-1 than for wild type (0.51 and 0.43 g ethanol/g glucose, respectively). With galacturonic acid as a carbon source, the ethanol yield after 7 d at 46 °C was higher for 7-1 than for wild type (0.48 and 0.34 g ethanol/g galacturonic acid, respectively). These mutant strains have potential application in fuel ethanol production at elevated temperature from sugar constituents of starch, sucrose, pectin, and cellulosic biomass.

Improved method of laser photovaporization for endometrium cases

NASA Astrophysics Data System (ADS)

Xia, En-ju; Lu, Hua; Chen, En-ling; Wan, Hong-yue

1995-03-01

Endometrium was destroyed by Nd-YAG laser under B ultrasonic monitoring through direct application of a fibrous catheter specially designed by Dong-lin Lee (an optical engineer). Of the 12 patients 11 cases had dysfunctional uterine bleeding and one case had postmenopausal uterine bleeding. The mean time of laser treatment was 5' 45', the mean volume of irrigating solution was 850 ml, and the mean estimated blood loss was less than 9 ml. All patients showed neither evidence of infection nor uterine perforation. Follow-up examination for 3 - 16 months showed amenorrhea in 4 cases, spotting in 4 cases, and obviously reduced menstrual flow in 3 cases. One patient, partly treated, had normal menstruation.

46 CFR 7.1 - General purpose of boundary lines.

Code of Federal Regulations, 2011 CFR

2011-10-01

... which are defined in 46 U.S.C. 2101(32); 46 U.S.C. 3301(7) requiring the inspection of seagoing motor... State of Washington; and 46 U.S.C. 8304, “Implementing the Officers' Competency Certificates Convention...

46 CFR 7.1 - General purpose of boundary lines.

Code of Federal Regulations, 2010 CFR

2010-10-01

... which are defined in 46 U.S.C. 2101(32); 46 U.S.C. 3301(7) requiring the inspection of seagoing motor... State of Washington; and 46 U.S.C. 8304, “Implementing the Officers' Competency Certificates Convention...

Genetic Analysis of 13 Iranian Families With Leukocyte Adhesion Deficiency Type 1.

PubMed

Teimourian, Shahram; De Boer, Martin; Roos, Dirk; Isaian, Anna; Bemanian, Mohammad Hassan; Lashkary, Sharhzad; Nabavi, Mohammad; Arshi, Saba; Nateghian, Alireza; Sayyahfar, Shirin; Sazgara, Faezeh; Taheripak, Gholamreza; Alipour Fayez, Elham

2018-05-10

Leukocyte adhesion deficiency type 1 is a rare, autosomal recessive disorder that results from mutations in the ITGB2 gene. This gene encodes the CD18 subunit of β2 integrin leukocyte adhesion cell molecules. Leukocyte adhesion deficiency type 1 is characterized by recurrent bacterial infections, impaired wound healing, inadequate pus formation, and delayed separation of the umbilical cord. Blood samples were taken from 13 patients after written consent had been obtained. Genomic DNA was extracted, and ITGB2 exons and exon-intron boundaries were amplified by polymerase chain reaction. The products were examined by Sanger sequencing. In this study, 8 different previously reported mutations (intron7+1G>A, c.715G>A, c.1777 C>T, c.843del C, c.1768T>C, c.1821C>A, Intron7+1G>A, c.1885G>A) and 2 novel mutations (c.1821C>A; p.Tyr607Ter and c.1822C>T; p.Gln608Ter) were found. c.1821C>A (p.Tyr607Ter) and c.1822C>T (p.Gln608Ter) mutations should be included in the panel of carrier detection and prenatal diagnosis.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yun, Dong-Jin; Chung, JaeGwan; Jung, Changhoon

The material arrangement and energy level alignment of an organic bilayer comprising of phenyl-c71-butyric-acid-methyl ester (PCBM-71) and pentacene were studied using ultraviolet photoelectron spectroscopy (UPS) and the argon gas cluster ion beam (GCIB) sputtering process. Although there is a small difference in the full width at half maximum of the carbon C 1s core level peaks and differences in the oxygen O 1s core levels of an X-ray photoemission spectroscopy spectra, these differences are insufficient to clearly distinguish between PCBM-71 and pentacene layers and to classify the interface and bulk regions. On the other hand, the valence band structures inmore » the UPS spectra contain completely distinct configurations for the PCBM-71 and pentacene layers, even when they have similar atomic compositions. According to the valence band structures of the PCBM-71/pentacene/electrodes, the highest unoccupied molecular orbital (HOMO) region of pentacene is at least 0.8 eV closer to the Fermi level than that of PCBM-71 and it does not overlap with any of the chemical states in the valence band structure of PCBM-71. Therefore, by just following the variations in the area of the HOMO region of pentacene, the interface/bulk regions of the PCBM/pentacene layers were distinctly categorized. Besides, the variation of valence band structures as a function of the Ar GCIB sputtering time fully corroborated with the surface morphologies observed in the atomic force microscope images. In summary, we believe that the novel approach, which involves UPS analysis in conjunction with Ar GCIB sputtering, can be one of the best methods to characterize the material distribution and energy level alignments of stacks of organic layers.« less

Summary of Meteorological Observations, Surface (SMOS) for Keflavik, Iceland

DTIC Science & Technology

1989-04-01

is not considered obstruction to vision for purposes of this summary; therefore, the percentage total of obstructions to vis need not reflect the total...1 .J . I0 . . 1 0 .0 .c IC 10 .5 CLP .’ .C .0 . : . .0 C0 1.2 .0 AL 3I.4 331. 3 2%. 1 ’* A$. p~t r~ r Iro C0 I03’. 2 T VI AL N. (3 0~A 10 t 42 p f W...55w ’LI. • b VI "w" Jut NOUN :0 n0 Lk 1 ):51 )il /4Il6 : J ): r , 7(2. 20.6 72.1 20.7 70.7 ,’ 7 1. 21.6 7.1 7 II-? 71.1 .S 22.( 22.6 72.7 27.7 72

Kunsan AB, Kunsan, Korea. Revised Uniform Summary of Surface Weather Observations (Russwo) Parts A-F.

DTIC Science & Technology

1977-10-27

Ch T ,, fC /, SAF SURFACE WINDS A I’!k I0TIA-ER SERv ICEI- /t PERCENTAGE FREQUENCY OF WIND DIRECTION AND SPEED (FROM HOURLY OBSERVATIONS) STATION STATION...9 .3 7. e i i, _ ,__ F ._ ss .71 .3; .8 b I .3 3’ a I 1 l.A 7.o w - 194 - _2 --- __ 1____ 1 .7 7.1 __ .4_.,.0 6. y NNW 4- . . .1 10 b. C 6.1 CALM...8217.. ’ . % ;. ) H--- ’• 3. :," , 7’ fC . ’ r;.7 97.’ ) L:. o i’, 9 ’. 9 C ’ " - 9 *.; 7. t) Q .IAo .... Ll 79. ... J .. _ ! i ¢Vo7i 9.9 90o7 97ou, ’,3 ; * I ,vq,9 99

A clinically authentic mouse model of enterovirus 71 (EV-A71)-induced neurogenic pulmonary oedema.

PubMed

Victorio, Carla Bianca Luena; Xu, Yishi; Ng, Qimei; Chua, Beng Hooi; Alonso, Sylvie; Chow, Vincent T K; Chua, Kaw Bing

2016-06-30

Enterovirus 71 (EV-A71) is a neurotropic virus that sporadically causes fatal neurologic illness among infected children. Animal models of EV-A71 infection exist, but they do not recapitulate in animals the spectrum of disease and pathology observed in fatal human cases. Specifically, neurogenic pulmonary oedema (NPE)-the main cause of EV-A71 infection-related mortality-is not observed in any of these models. This limits their utility in understanding viral pathogenesis of neurologic infections. We report the development of a mouse model of EV-A71 infection displaying NPE in severely affected animals. We inoculated one-week-old BALB/c mice with an adapted EV-A71 strain and identified clinical signs consistent with observations in human cases and other animal models. We also observed respiratory distress in some mice. At necropsy, we found their lungs to be heavier and incompletely collapsed compared to other mice. Serum levels of catecholamines and histopathology of lung and brain tissues of these mice strongly indicated onset of NPE. The localization of virally-induced brain lesions also suggested a potential pathogenic mechanism for EV-A71-induced NPE. This novel mouse model of virally-induced NPE represents a valuable resource for studying viral mechanisms of neuro-pathogenesis and pre-clinical testing of potential therapeutics and prophylactics against EV-A71-related neurologic complications.

Severe paediatric conditions linked with EV-A71 and EV-D68, France, May to October 2016

PubMed Central

Antona, Denise; Kossorotoff, Manoëlle; Schuffenecker, Isabelle; Mirand, Audrey; Leruez-Ville, Marianne; Bassi, Clément; Aubart, Mélodie; Moulin, Florence; Lévy-Bruhl, Daniel; Henquell, Cécile; Lina, Bruno; Desguerre, Isabelle

2016-01-01

We report 59 cases of severe paediatric conditions linked with enterovirus (EV)-A71 and EV-D68 in France between May and October 2016. Fifty-two children had severe neurological symptoms. EV sequence-based typing for 42 cases revealed EV-A71 in 21 (18 subgenotype C1, detected for the first time in France) and EV-D68 in eight. Clinicians should be encouraged to obtain stool and respiratory specimens from patients presenting with severe neurological disorders for EV detection and characterisation. PMID:27918268

Examining the Screen for Child Anxiety-Related Emotional Disorder-71 as an assessment tool for anxiety in children with high-functioning autism spectrum disorders.

PubMed

van Steensel, Francisca J A; Deutschman, Amber A C G; Bögels, Susan M

2013-11-01

The psychometric properties of a questionnaire developed to assess symptoms of anxiety disorders (SCARED-71) were compared between two groups of children: children with high-functioning autism spectrum disorder and comorbid anxiety disorders (ASD-group; n = 115), and children with anxiety disorders (AD-group; n = 122). Anxiety disorders were established with a semi-structured interview (ADIS-C/P), using child- as well as parent-report. Internal consistency, construct validity, sensitivity, specificity, and discriminant validity of the SCARED-71 was investigated. Results revealed that the psychometric properties of the SCARED-71 for the ASD-group were quite comparable to the AD-group, however, the discriminant validity of the SCARED-71 child-report was less in the ASD-group. Raising the parental cutoffs of the SCARED-71 resulted in higher specificity rates, which suggests that research should focus more on establishing alternative cutoffs for the ASD-population.

21 CFR 201.70 - Calcium labeling.

Code of Federal Regulations, 2013 CFR

2013-04-01

... stones [bullet] a calcium-restricted diet”. The warnings in §§ 201.64(c), 201.70(c), 201.71(c), and 201...., a calcium or sodium restricted diet. 1 See § 201.66(b)(4) of this chapter for definition of bullet...

21 CFR 201.70 - Calcium labeling.

Code of Federal Regulations, 2014 CFR

2014-04-01

... stones [bullet] a calcium-restricted diet”. The warnings in §§ 201.64(c), 201.70(c), 201.71(c), and 201...., a calcium or sodium restricted diet. 1 See § 201.66(b)(4) of this chapter for definition of bullet...

21 CFR 201.70 - Calcium labeling.

Code of Federal Regulations, 2012 CFR

2012-04-01

... stones [bullet] a calcium-restricted diet”. The warnings in §§ 201.64(c), 201.70(c), 201.71(c), and 201...., a calcium or sodium restricted diet. 1 See § 201.66(b)(4) of this chapter for definition of bullet...

Expression and permeation properties of the K(+) channel Kir7.1 in the retinal pigment epithelium.

PubMed

Shimura, M; Yuan, Y; Chang, J T; Zhang, S; Campochiaro, P A; Zack, D J; Hughes, B A

2001-03-01

Bovine Kir7.1 clones were obtained from a retinal pigment epithelium (RPE)-subtracted cDNA library. Human RPE cDNA library screening resulted in clones encoding full-length human Kir7.1. Northern blot analysis indicated that bovine Kir7.1 is highly expressed in the RPE. Human Kir7.1 channels were expressed in Xenopus oocytes and studied using the two-electrode voltage-clamp technique. The macroscopic Kir7.1 conductance exhibited mild inward rectification and an inverse dependence on extracellular K+ concentration ([K+]o). The selectivity sequence based on permeability ratios was K+ (1.0) approximately Rb+ (0.89) > Cs+ (0.013) > Na+ (0.003) approximately Li+ (0.001) and the sequence based on conductance ratios was Rb+ (9.5) > K+ (1.0) > Na+ (0.458) > Cs+ (0.331) > Li+ (0.139). Non-stationary noise analysis of Rb+ currents in cell-attached patches yielded a unitary conductance for Kir7.1 of approximately 2 pS. In whole-cell recordings from freshly isolated bovine RPE cells, the predominant current was a mild inwardly rectifying K+ current that exhibited an inverse dependence of conductance on [K+]o. The selectivity sequence based on permeability ratios was K+ (1.0) approximately Rb+ (0.89) > Cs+ (0.021) > Na+ (0.003) approximately Li+ (0.002) and the sequence based on conductance ratios was Rb+ (8.9) > K+ (1.0) > Na+ (0.59) > Cs+ (0.23) > Li+ (0.08). In cell-attached recordings with Rb+ in the pipette, inwardly rectifying currents were observed in nine of 12 patches of RPE apical membrane but in only one of 13 basolateral membrane patches. Non-stationary noise analysis of Rb+ currents in cell-attached apical membrane patches yielded a unitary conductance for RPE Kir of approximately 2 pS. On the basis of this molecular and electrophysiological evidence, we conclude that Kir7.1 channel subunits comprise the K+ conductance of the RPE apical membrane.

Camp Casey, Tongduchon, Korea. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F

DTIC Science & Technology

1981-02-10

76.,a41 7S.4 ?R.4’ 7B.q. .4 . I 78.uf 7-.t- . .I 2 V_𔃻 77.71 77.71 72.2 f6 . I 7 -.. 4 78. . ,= , 9Ss 7. 7. ’ 7 .Z 7 7 79.Z 79.2 79.1-3; 77. 7 79.a: 75...cof-. .4r E0C >j a- -- =* C.j C . = ± (i3e!’ afl o oc- g Nta.. a .o.r3azdflloD2Coa.aoc a- f~ie~~z. n.!. G a 4 . ACca . . rx Co.--&Z3Er,0C i1DaI Da...6-=.1 66.4 ;-’ -0 o-b 915 c.01 is 99.2i 90.7 99.7 9 9. 1 99.7s. JO 200 1 5 01 6 ,. 1 66.4! .69 F6 . 1 ? 1 .61 976.1if 97.r1 99. 1 9gs 999 .39 a c .3 c

40 CFR 280.71 - Permanent closure and changes-in-service.

Code of Federal Regulations, 2011 CFR

2011-07-01

... UNDERGROUND STORAGE TANKS (UST) Out-of-Service UST Systems and Closure § 280.71 Permanent closure and changes... sludges. All tanks taken out of service permanently must also be either removed from the ground or filled with an inert solid material. (c) Continued use of an UST system to store a non-regulated substance is...

40 CFR 280.71 - Permanent closure and changes-in-service.

Code of Federal Regulations, 2013 CFR

2013-07-01

... UNDERGROUND STORAGE TANKS (UST) Out-of-Service UST Systems and Closure § 280.71 Permanent closure and changes... sludges. All tanks taken out of service permanently must also be either removed from the ground or filled with an inert solid material. (c) Continued use of an UST system to store a non-regulated substance is...

40 CFR 280.71 - Permanent closure and changes-in-service.

Code of Federal Regulations, 2012 CFR

2012-07-01

... UNDERGROUND STORAGE TANKS (UST) Out-of-Service UST Systems and Closure § 280.71 Permanent closure and changes... sludges. All tanks taken out of service permanently must also be either removed from the ground or filled with an inert solid material. (c) Continued use of an UST system to store a non-regulated substance is...

40 CFR 280.71 - Permanent closure and changes-in-service.

Code of Federal Regulations, 2014 CFR

2014-07-01

... UNDERGROUND STORAGE TANKS (UST) Out-of-Service UST Systems and Closure § 280.71 Permanent closure and changes... sludges. All tanks taken out of service permanently must also be either removed from the ground or filled with an inert solid material. (c) Continued use of an UST system to store a non-regulated substance is...

75 FR 7031 - Notice of Determinations Regarding Eligibility To Apply for Worker Adjustment Assistance

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-16

... investigation resulting in-- (A) An affirmative determination of serious injury or threat thereof under section... Connection and Manpower, Vadnais Heights, MN, June 30, 2008. TA-W-71,807; B & C Machine Company, Barberton..., Northwest Division, Renton, WA, June 5, 2008. TA-W-71,258; UPM Raflatac, Inc., Leased Workers from Manpower...

48 CFR 1252.228-71 - Fair market value of aircraft.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Fair market value of....228-71 Fair market value of aircraft. As prescribed in (TAR) 48 CFR 1228.306-70(a) and (c), insert the following clause: Fair Market Value of Aircraft (OCT 1994) For purposes of the clause entitled “Loss of or...

Unconventional Superconductivity in the BiS_{2}-Based Layered Superconductor NdO_{0.71}F_{0.29}BiS_{2}.

PubMed

Ota, Yuichi; Okazaki, Kozo; Yamamoto, Haruyoshi Q; Yamamoto, Takashi; Watanabe, Shuntaro; Chen, Chuangtian; Nagao, Masanori; Watauchi, Satoshi; Tanaka, Isao; Takano, Yoshihiko; Shin, Shik

2017-04-21

We investigate the superconducting-gap anisotropy in one of the recently discovered BiS_{2}-based superconductors, NdO_{0.71}F_{0.29}BiS_{2} (T_{c}∼5  K), using laser-based angle-resolved photoemission spectroscopy. Whereas the previously discovered high-T_{c} superconductors such as copper oxides and iron-based superconductors, which are believed to have unconventional superconducting mechanisms, have 3d electrons in their conduction bands, the conduction band of BiS_{2}-based superconductors mainly consists of Bi 6p electrons, and, hence, the conventional superconducting mechanism might be expected. Contrary to this expectation, we observe a strongly anisotropic superconducting gap. This result strongly suggests that the pairing mechanism for NdO_{0.71}F_{0.29}BiS_{2} is an unconventional one and we attribute the observed anisotropy to competitive or cooperative multiple paring interactions.

Tracing metal sources in core sediments of the artificial lake An-Dong, Korea: Concentration and metal association.

PubMed

Choi, Mansik; Park, Jongkyu; Cho, Dongjin; Jang, Dongjun; Kim, Miseon; Choi, Jongwoo

2015-09-15

The concentration and source of trace metals in the artificial lake An-Dong, which has widespread abandoned mines and a Zn smelter upstream of the drainage basin, were investigated. Soils (18ea), stream waters (15ea) and sediments (15ea) in the main channel and five tributaries downstream of the Zn smelter towards the lake (~ 50 km downstream) were collected. And two core sediments were also taken from the middle of the lake. All samples were analyzed for trace metals in bulk and in a 1N HCl-leached fraction. Although the soil and stream sediments consisted mostly of sand-sized grains, concentrations of metals (Cu, Zn, Cd and Pb) were very high in all samples, including soils, stream waters and sediments at sites near the Zn smelter. However the metal concentrations decreased rapidly downstream, suggesting that the area of impact of the smelter lies within 5 km. Highly enriched metal concentrations were also found in dated core sediments from the lake; while the highest concentrations of Co, Ni, As, Cu, Zn, Cd and Pb were detected in the bottom of the sediment core (dated 1980) they decreased towards 2000, and only Cu, Zn and Cd concentrations increased again in present-day samples. Since the temporal variation in metal concentrations appeared consistent with historical variation in ore mining and Zn smelter production rates, a model combining the production rates of each was developed, which estimated 3%, 12% and 7% contributions from Zn smelter compared to ore mining production rate to levels of Cu, Cd and Zn, respectively, suggesting the different pathways by different sources. In addition, analysis of Cd/Zn and Cu/Zn ratios showed that contamination from ore mining decreased from 1980 to 2000, and smelting processes were most likely responsible for metal enrichment (Cu, Cd and Zn) from 2000 to the present. Copyright © 2015 Elsevier B.V. All rights reserved.

Study on Sources of Volatile Organic Compounds (CMB) in Pearl River Delta region, China

NASA Astrophysics Data System (ADS)

Liu, Y.; Shao, M.; Lu, S.; Chang, C.; Wang, C. J.; Wang, B.

2007-05-01

The profiles of major Volatile organic compounds (VOCs) sources including vehicle exhaust, gasoline vapor, painting, asphalt, liquefied petroleum gas (LPG), biomass burning and petrochemical industry in Pearl River Delta were experimentally determined. Source samples were taken by using dilution chamber for mobile and stationary sources, laboratory simulation for biomass burning. The concentrations of 108 VOC species of sources were quantified by using canister with pre-concentration-GC/MS system, from which 52 PAMS hydrocarbons and one kind of chlorinated hydrocarbon were deployed to build the source profiles for source apportionment of VOCs. Based the measurement of source profiles, the possible tracers for various emission sources were identified, e.g 2-methylbutane and 1,3-butadiene were the tracers for motor vehicle exhaust, the characteristic compounds of architectural and furnishing coatings are aromatics such as toluene and m/p-xylene; the light hydrocarbons, namely n-butane, trans-2-butene and n-pentane, dominated the composition of gasoline vapor; and the nonane, decane and undecane are found to represent the asphalt emissions etc.. The CMB receptor model was applied to source apportionment of 58 hydrocarbons measured at seven sites during the PRD campaign, 2004. The 12 kinds of VOC sources include gasoline/diesel-powered vehicle exhaust, gasoline/diesel headspace vapor, vehicle evaporative emissions, liquid petroleum gas (LPG) leakage, painting vapors, asphalt emission from paved road, biomass burning, coal burning, chemical industry and petroleum refinery. Vehicle exhaust was the largest sources contributing over half of the ambient VOCs at the three urban sites (GuangZhou, FoShan and ZhongShan). LPG leakage played an important role with the percentage of 8- 16% in most sites in PRD. Contributions from solvents usage were highest at DongGuan, an industrial site. At XinKen, the solvents and coatings had the largest percentage of 31% probably due to the influence of its upwind area of DongGuan. The local biomass burning was also found to be a noticeable source at XK.

Evaluation of the Efficacy and Safety of DA-9601 versus Its New Formulation, DA-5204, in Patients with Gastritis: Phase III, Randomized, Double-Blind, Non-Inferiority Study.

PubMed

Choi, Yoon Jin; Lee, Dong Ho; Choi, Myung Gyu; Lee, Sung Joon; Kim, Sung Kook; Song, Geun Am; Rhee, Poong Lyul; Jung, Hwoon Yong; Kang, Dae Hwan; Lee, Yong Chan; Lee, Si Hyung; Choi, Suck Chei; Shim, Ki Nam; Seol, Sang Yong; Moon, Jeong Seop; Shin, Yong Woon; Kim, Hyun Soo; Lee, Soo Teik; Cho, Jin Woong; Choi, Eun Kwang; Lee, Oh Young; Jang, Jin Seok

2017-11-01

This study compared the efficacy of DA-9601 (Dong-A ST Co., Seoul, Korea) and its new formulation, DA-5204 (Dong-A ST Co.), for treating erosive gastritis. This phase III, randomized, multicenter, double-blind, non-inferiority trial randomly assigned 434 patients with endoscopically proven gastric mucosal erosions into two groups: DA-9601 3 times daily or DA-5,204 twice daily for 2 weeks. The final analysis included 421 patients (DA-5204, 209; DA-9601, 212). The primary endpoint (rate of effective gastric erosion healing) and secondary endpoints (cure rate of endoscopic erosion and gastrointestinal [GI] symptom relief) were assessed using endoscopy after the treatment. Drug-related adverse events (AEs), including GI symptoms, were also compared. At week 2, gastric healing rates with DA-5204 and DA-9601 were 42.1% (88/209) and 42.5% (90/212), respectively. The difference between the groups was -0.4% (95% confidence interval, -9.8% to 9.1%), which was above the non-inferiority margin of -14%. The cure rate of gastric erosion in both groups was 37.3%. The improvement rates of GI symptoms with DA-5204 and DA-9601 were 40.4% and 40.8%, respectively. There were no statistically significant differences between the two groups in both secondary endpoints. AEs were reported in 18 (8.4%) patients in the DA-5204 group and 19 (8.8%) in the DA-9601 group. Rates of AE were not different between the two groups. No serious AE or adverse drug reaction (ADR) occurred. These results demonstrate the non-inferiority of DA-5204 compared to DA-9601. DA-5204 is as effective as DA-9601 in the treatment of erosive gastritis. Registered randomized clinical trial at ClinicalTrials.gov (NCT02282670). © 2017 The Korean Academy of Medical Sciences.

Simulating hydrological processes of a typical small mountainous catchment in Tibetan Plateau

NASA Astrophysics Data System (ADS)

Xu, Y. P.; Bai, Z.; Fu, Q.; Pan, S.; Zhu, C.

2017-12-01

Water cycle of small watersheds with seasonal/permanent frozen soil and snow pack in Tibetan Plateau is seriously affected by climate change. The objective of this study is to find out how much and in what way the frozen soil and snow pack will influence the hydrology of small mountainous catchments in cold regions and how can the performance of simulation by a distributed hydrological model be improved. The Dong catchment, a small catchment located in Tibetan Plateau, is used as a case study. Two measurement stations are set up to collect basic meteorological and hydrological data for the modeling purpose. Annual and interannual variations of runoff indices are first analyzed based on historic data series. The sources of runoff in dry periods and wet periods are analyzed respectively. Then, a distributed hydrology soil vegetation model (DHSVM) is adopted to simulate the hydrological process of Dong catchment based on limited data set. Global sensitivity analysis is applied to help determine the important processes of the catchment. Based on sensitivity analysis results, the Epsilon-Dominance Non-Dominated Sorted Genetic Algorithm II (ɛ-NSGAII) is finally added into the hydrological model to calibrate the hydrological model in a multi-objective way and analyze the performance of DHSVM model. The performance of simulation is evaluated with several evaluation indices. The final results show that frozen soil and snow pack do play an important role in hydrological processes in cold mountainous region, in particular in dry periods without precipitation, while in wet periods precipitation is often the main source of runoff. The results also show that although the DHSVM hydrological model has the potential to model the hydrology well in small mountainous catchments with very limited data in Tibetan Plateau, the simulation of hydrology in dry periods is not very satisfactory due to the model's insufficiency in simulating seasonal frozen soil.

Detection of non-polio enteroviruses in Hungary 2000-2008 and molecular epidemiology of enterovirus 71, coxsackievirus A16, and echovirus 30.

PubMed

Kapusinszky, Beatrix; Szomor, Katalin N; Farkas, Agnes; Takács, Mária; Berencsi, György

2010-04-01

Human enteroviruses are associated with various clinical syndromes from minor febrile illness to severe, potentially fatal conditions like aseptic meningitis, paralysis, myocarditis, and neonatal enteroviral sepsis. Between June 2000 and August 2008 echovirus (E) type 2, 4, 6, 7, 9, 11, 13, 25, 30, coxsackievirus (CV) -A16, -A19, -B5, and enterovirus 71 (EV71) were reported in Hungary. In this study, 29 previously enterovirus positive samples from 28 patients diagnosed with hand, foot and mouth disease, meningitis and encephalitis, were molecularly typed. The genetic relationships of identified serotypes CV-A16, EV71, and E30 were assessed by direct sequencing of genomic region encoding the capsid protein VP1. The sequences were compared to each other and sequences from other geographical regions possessed in Genbank. The phylogenetic analysis of CV-A16 revealed that the viruses were mostly of Far-Eastern or Asia-Pacific origin. Typing of EV71 showed that one virus from 2000 belonged to genotype C1 and five viruses observed in 2004 and 2005 were identified as genotype C4. The 11 echovirus 30 strains showed homology with those of neighbor European countries. The molecular examination of E30 revealed that three separate lineages circulated in 2000, 2001, and 2004-2006 in Hungary.

Dollar Summary of Prime Contract Awards by State, Place, and Contractor, FY84, Part 2 (Air Force Academy, Colorado-Zion, Illinois).

DTIC Science & Technology

1984-01-01

THEODORE AND COMPANY 32 32 HOLDING HAROLD R 71 71 INTERACTIVE SYSTEMS CORP 297 297 J & R CONSTRUCTION CO INC 94 94 KETCHUM KONKEL & AUSTIN 10 150 MC...TELECOM INC 510 510 DATA SYSTEMS MARKETING 26 26 DUGGAN PETROLEUM CO INC 3,249 3,249 HONEYWELL INC 3,581 267 2,400 830 INTERACTIVE SYSTEMS CORP 424 424...FORCE DLA OCE ODA SANDERSVILLE PRENTISS DRUG & CHEMICAL CO 89 89 TOTAL - SANDERSVILLE 744 744 SAVANNAH A C S CONSTRUCTION CO MISS 550 550 A J C INTL

Automatic Pulse Shaping with the AN/FPN-42 and AN/FPN-44A Loran-C transmitters

DTIC Science & Technology

1992-12-01

with antenna simulator, pair 30. (a) TDW and (b) RF pulse. 39 CLOSEUP: POWER SPECTRUM OF TOW & RF (PAIR 30), 47 XMTR 190 17025 " 3 0 4 0 5 6 Sapl numbr... iec X 1e-6 (a) Phase Vf Selected Parameter 0.057 0.5 ... .. . . . ......... .... .. . ............... ,.. ...-.. . , .... celurro, 3 0.0517...PAIR 7 1), "SA XMTR ISO IS 25 30 35 40 4 0 55 60 Sample number, k Figure 3.15c: Closeup of power spectrum, 144A, pair 71i. 77 POLE/ZERO PLOT (PAIR 71

Grafting of Polyaniline onto the Surface of 4-Aminobenzoyl-Functionalized Multiwalled Carbon Nanotube and Its Electrochemical Properties

DTIC Science & Technology

2010-01-01

MWNT was conducted in ammonium persulfate (APS)/1 M aqueous hydrochloric acid (HCl) to yield PANi-g-MWNT. The resultant composites showed improved...poured into water. The precipitates were collected by suction filtra- tion and Soxhlet extracted with distilled water for 3 days and methanol for 3 days...and finally freeze-dried for 48 h to yield 9.82 g (71.7% yield ) of dark black powder. Anal. Calcd. for C12.71H6O1N1: C, 80.89%; H, 3.21%; N, 7.42

Evaluation of a rapid method for measurement of catalase activity in cooked beef and sausage.

PubMed

Davis, C E; Cyrus, S

1998-02-01

Catalase (CAT) activity in ground beef and pork was determined on samples cooked from 60 to 71.1 degrees C. One-gram samples of ground round (4% fat), hamburger (24% fat), and commercial pork sausage (38%fat) were cooked in a controlled-temperature waterbath at 65, 68.3 and 71 degrees C. Chilled samples were immersed in direct contact with the cooking water; the test samples were removed every 15 s and immediately immersed in an ice-water bath (O to 1 degrees C) to quick-chill the samples to prevent temperature over-run. Samples retained high (HMB value 20+, over range) CAT activity through 90, 60, and 45 s at 65, 68.3, and 71 degrees C, respectively, before showing rapid activity decreases. Four USDA-FSIS approved meat patty heating processes (66.1 degrees C, 41 s; 67.2 degrees C, 26 s; 68.3 degrees C, 16 s; and 69.4 degrees C, 10 s) were analyzed for CAT activity in meat frozen prior to cooking was slightly lower (P < 0.05) than in degrees C meat. CAT activity decreased (P < 0.05) among meat treated at 66.1 degrees C for 41 s, at 67.2 degrees C for 26 s, and at 68.3 degrees C for 16 s, but the treatment at 68.3 degrees C for 16 s was not different (P < 0.05) from that at 69.4 degrees C for 10 s. These results show this rapid (20 to 25 min) CAT activity test could be used to establish activity values at specific end-point temperatures for model heat-processed ground beef or sausage products and may be useful to USDA FSIS process inspectors and food processors in quality assurance and HACCP (hazard analysis critical control points) programs for thermal input verification.

Cookery method and endpoint temperature can affect the Warner-Bratzler shear force, cooking loss, and internal cooked color of beef semimembranosus and infraspinatus steaks.

PubMed

Yancey, J W S; Apple, J K; Wharton, M D

2016-10-01

Steaks from USDA Select inside rounds (Exp. 1) and shoulder clods (Exp. 2) were used to test the interactive effect of cookery method and endpoint temperature on Warner-Bratzler shear force (WBSF) and internal cooked color. Pairs of 2.5-cm-thick semimembranosus (SM) or infraspinatus (INF) steaks ( = 360/muscle) were cut from each subprimal, labeled, vacuum packaged, and frozen at -30°C in the dark for approximately 60 d before being cooked to 65.5, 71.1, or 76.6°C using 1) a forced-air convection oven (FAC); 2) a forced-air impingement oven (IMP); 3) a gas-fired, open-hearth charbroiler (CHAR); 4) an electric countertop griddle (GRID); or 5) a clam-shell grill (CLAM). Thawed steaks were cooked to their assigned endpoint temperature × cookery method combination, and, after a 5-min cooling period, steaks were weighed to calculate cooking loss percentage and subsequently sliced perpendicular to the cut surface to measure instrumental cooked color. Then, 6 cores were removed for measurement of WBSF. Cooking losses of SM steaks increased ( < 0.05) with each increase in endpoint temperature, whereas INF steaks cooked on a CHAR had the greatest ( < 0.05) cooking losses and cooking INF steaks with the GRID and the CLAM resulted in lesser ( < 0.05) cooking losses than cooking with the FAC and the IMP. Cooking SM steaks on the CHAR resulted in greater ( < 0.05) WBSF values than all other cookery methods when cooked to 65.5 and 76.6°C and greater ( < 0.05) WBSF values than those cooked on the FAC, GRID, and CLAM when cooked to 71.1°C. Shear force values were greater ( < 0.05) for INF steaks cooked to 71.1 and 76.6°C than those cooked to 65.5°C, but INF WBSF values were similar ( = 0.55) among cookery methods. At 65.5°C, FAC-cooked SM steaks were redder ( < 0.05) than those cooked with the GRID and the IMP and, at 71.1°C, CLAM-cooked SM steaks were redder ( < 0.05) than FAC- and IMP-cooked SM steaks; however, a* values were similar ( > 0.05) among cookery methods when cooked to 76.6°C. Redness did not ( > 0.05) differ among INF steaks cooked to 65.5 and 71.1°C with the FAC and the CHAR, whereas internal color of INF steaks cooked in the IMP and the FAC was redder ( < 0.05) than that of INF steaks cooked with the CLAM and the GRID to 76.6°C. Results suggest that endpoint temperature has a greater impact on cooking properties of SM and INF steaks than cookery method, yet it is apparent that internal cooked color of INF and SM steaks react differently to some cookery method-endpoint temperature combinations.

Variation in Lake Michigan alewife (Alosa pseudoharengus) thiaminase and fatty acids composition

USGS Publications Warehouse

Honeyfield, D.C.; Tillitt, D.E.; Fitzsimons, J.D.; Brown, S.B.

2010-01-01

Thiaminase activity of alewife (Alosa pseudoharengus) is variable across Lake Michigan, yet factors that contribute to the variability in alewife thiaminase activity are unknown. The fatty acid content of Lake Michigan alewife has not been previously reported. Analysis of 53 Lake Michigan alewives found a positive correlation between thiaminase activity and the following fatty acid: C22:ln9, sum of omega-6 fatty acids (Sw6), and sum of the polyunsaturated fatty acids. Thiaminase activity was negatively correlated with C15:0, C16:0, C17:0, C18:0, C20:0, C22:0, C24:0, C18:ln9t, C20:3n3, C22:2, and the sum of all saturated fatty acids (SAFA). Multi-variant regression analysis resulted in three variables (C18:ln9t, Sw6, SAFA) that explained 71% (R2=0.71, P<0.0001) of the variation in thiaminase activity. Because the fatty acid content of an organism is related is food source, diet may be an important factor modulating alewife thiaminase activity. These data suggest there is an association between fatty acids and thiaminase activity in Lake Michigan alewife.

IN VIVO AND IN VITRO ANTI-ANDROGENIC EFFECTS OF DE-71, A COMMERCIAL POLYBROMINATED DIPHENYL ETHER MIXTURE, IN THE RAT

EPA Science Inventory

In Vivo and In Vitro Anti-androgenic Effects of DE-71, A Commerical Polybrominated Diphenyl Ether (PBDE) Mixture.

Stoker, T.E., Lambright, C.S. and Gray, L.E.
Endocrinology Branch, RTD, NHEERL, ORD, U.S. EPA, Research Triangle Park, NC.

PBDEs are synthesized in...

Status of Storm Water Pollution in the United States

DTIC Science & Technology

1994-09-01

71 Appendix C: Air Force Bases That Applied for the Group Permit ......................... 72 Appendix D: E-Mail Message...34......................................... 25 8. Pollutant Bench Mark Values ....................... 26 9. Air Force Bases Impacted by the Proposed "NPDES General Permit For Storm Water...Metals, Cyanide, Phenols .......................... 70 24. Toxic Pollutants and Hazardous Substances ......... 71 25. Air Force Bases That Applied For

29 CFR 71.51 - Specific exemptions pursuant to subsection (k)(2) of the Privacy Act.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 1 2010-07-01 2010-07-01 true Specific exemptions pursuant to subsection (k)(2) of the... § 71.51 Specific exemptions pursuant to subsection (k)(2) of the Privacy Act. (a) The following systems of records are eligible for exemption under 5 U.S.C. 552a(k)(2) because they contain investigatory...

29 CFR 71.52 - Specific exemptions pursuant to subsection (k)(5) of the Privacy Act.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 1 2010-07-01 2010-07-01 true Specific exemptions pursuant to subsection (k)(5) of the... § 71.52 Specific exemptions pursuant to subsection (k)(5) of the Privacy Act. (a) The following systems of records are eligible for exemption under 5 U.S.C. 552a(k)(5) because they contain investigatory...

Draft Genome Sequence of the Psychrophilic and Alkaliphilic Rhodonellum psychrophilum Strain GCM71T.

PubMed

Hauptmann, Aviaja L; Glaring, Mikkel A; Hallin, Peter F; Priemé, Anders; Stougaard, Peter

2013-12-05

Rhodonellum psychrophilum GCM71(T), isolated from the cold and alkaline submarine ikaite columns in the Ikka Fjord in Greenland, displays optimal growth at 5 to 10°C and pH 10. Here, we report the draft genome sequence of this strain, which may provide insight into the mechanisms of adaptation to these extreme conditions.

78 FR 78302 - Proposed Modification and Establishment of Air Traffic Service (ATS) Routes in the Vicinity of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-26

... file V-243 are being vectored between the Bowling Green, KY (BWG), VOR Tactical Air Navigation (VORTAC... Administration proposes to amend 14 CFR part 71 as follows: PART 71--DESIGNATION OF CLASS A, B, C, D, AND E...] radials; Choo Choo; to Bowling Green, KY. * * * * * Paragraph 6011 United States Area Navigation Routes...

The Dependence of Donor:Acceptor Ratio on the Photovoltaic Performances of Blended poly (3-octylthiophene-2,5-diyl) and (6,6)-phenyl C{sub 71} butyric acid methyl ester Bulk Heterojunction Organic Solar Cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fauzia, Vivi; Institute of Microengineering and Nanoelectronics; Umar, Akrajas Ali

2010-10-24

Bulk heterojunction organic solar cells using blended poly (3-octylthiophene-2,5-diyl)(P3OT) and (6,6)-phenyl C{sub 71} butyric acid methyl ester (PC{sub 71}BM) have been fabricated. P3OT and PC{sub 71}BM were used as the electron donor (D) and acceptor (A), respectively. Both materials were mixed and dissolved in dichlorobenzene with three different D:A ratios i.e. 1:3, 1:1 and 3:1 (weight) while maintained at the concentration of 2 wt%(26 mg/ml). The blended thin films were sandwiched between the indium tin oxide (ITO) coated glass and the aluminum film. This paper reports the influence of donor:acceptor ratio on the performance of solar cell devices measured bymore » current-voltage measurement both in the dark and under 1.5 AM solar illumination. It was found that all devices showed the photovoltaic effect with poor diode behavior and the donor:acceptor ratio significantly influenced on the performance of bulk heterojunction organic solar cells.« less

45 CFR 86.71 - Interim procedures.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) Equipment, 86.41(c) Expenditures, 86.41(c) Facilities, 86.41(c) Travel, 86.41(c) Scholarships, [64, 65]; 86...-time employees, [89] G Grievance Procedure, see “Designation of responsible employee”, 86.8(a) (b) H... Undergraduate Professional Schools, [30]; 86.15(d) Purpose of Regulation, [13]; 86.1 R Real Property, 86.2(g...

Severe paediatric conditions linked with EV-A71 and EV-D68, France, May to October 2016.

PubMed

Antona, Denise; Kossorotoff, Manoëlle; Schuffenecker, Isabelle; Mirand, Audrey; Leruez-Ville, Marianne; Bassi, Clément; Aubart, Mélodie; Moulin, Florence; Lévy-Bruhl, Daniel; Henquell, Cécile; Lina, Bruno; Desguerre, Isabelle

2016-11-17

We report 59 cases of severe paediatric conditions linked with enterovirus (EV)-A71 and EV-D68 in France between May and October 2016. Fifty-two children had severe neurological symptoms. EV sequence-based typing for 42 cases revealed EV-A71 in 21 (18 subgenotype C1, detected for the first time in France) and EV-D68 in eight. Clinicians should be encouraged to obtain stool and respiratory specimens from patients presenting with severe neurological disorders for EV detection and characterisation. This article is copyright of The Authors, 2016.

Heat Resisting Metals for Gas Turbine Parts (N-102) - Chromium- Base Alloys

DTIC Science & Technology

1945-11-28

Copy No, 69 - a. F. Killer Cory No. 68 - 71 . L. B-dgor Copy No. 70 - N. U t’.ochol Members of tho flor Uotdtoirgy Coroitteo Copy Copy Ccpy...Copy Cory Copy Copy Copy Copy Copy Cory Copy Copy Cory Nc. 71 - Ctrl Broor No. 72 t Lyaen J. Briggs Nc. 73 - J. HOB H« C*itchott No, 74...Col, R.S.A. •Dcugh&rty No, 75 - Rudolph Furrer- < Nc. : 5 - H< 71 « Glllett No. 6 - S. D, Horen No. 76 - R. P. Houor • • < Nc. 7 - Zry Jeffries Nc

The Tegument Protein UL71 of Human Cytomegalovirus Is Involved in Late Envelopment and Affects Multivesicular Bodies ▿

PubMed Central

Schauflinger, Martin; Fischer, Daniela; Schreiber, Andreas; Chevillotte, Meike; Walther, Paul; Mertens, Thomas; von Einem, Jens

2011-01-01

Morphogenesis of human cytomegalovirus (HCMV) is still only partially understood. We have characterized the role of HCMV tegument protein pUL71 in viral replication and morphogenesis. By using a rabbit antibody raised against the C terminus of pUL71, we could detect the protein in infected cells, as well as in virions showing a molecular mass of approximately 48 kDa. The expression of pUL71, detected as early as 48 h postinfection, was not blocked by the antiviral drug foscarnet, indicating an early expression. The role of pUL71 during virus replication was investigated by construction and analysis of a UL71 stop mutant (TBstop71). The mutant could be reconstituted on noncomplementing cells proving that pUL71 is nonessential for virus replication in human fibroblasts. However, the inhibition of pUL71 expression resulted in a severe growth defect, as reflected by an up to 16-fold reduced extracellular virus yield after a high-multiplicity infection and a small-plaque phenotype. Ultrastructural analysis of cells infected with TBstop71 virus revealed an increased number of nonenveloped nucleocapsids in the cytoplasm, many of them at different stages of envelopment, indicating that final envelopment of nucleocapsids in the cytoplasm was affected. In addition, enlarged multivesicular bodies (MVBs) were found in close proximity to the viral assembly compartment, suggesting that pUL71 affects MVBs during virus infection. The observation of numerous TBstop71 virus particles attached to MVB membranes and budding processes into MVBs indicated that these membranes can be used for final envelopment of HCMV. PMID:21289123

Holographic entanglement for Chern-Simons terms

NASA Astrophysics Data System (ADS)

Azeyanagi, Tatsuo; Loganayagam, R.; Ng, Gim Seng

2017-02-01

We derive the holographic entanglement entropy contribution from pure and mixed gravitational Chern-Simons(CS) terms in AdS2 k+1. This is done through two different methods: first, by a direct evaluation of CS action in a holographic replica geometry and second by a descent of Dong's derivation applied to the corresponding anomaly polynomial. In lower dimensions ( k = 1 , 2), the formula coincides with the Tachikawa formula for black hole entropy from gravitational CS terms. New extrinsic curvature corrections appear for k ≥ 3: we give explicit and concise expressions for the two pure gravitational CS terms in AdS7 and present various consistency checks, including agreements with the black hole entropy formula when evaluated at the bifurcation surface.

Grand Forks Air Force Base, North Dakota. Revised Uniform Summary of Surface Weather Observations. Parts A-F.

DTIC Science & Technology

1987-11-01

centerlne rnvy 17. s Noy 69 1. no dong. Sans W-36 Sums "mq I a9APITA 0-I I OLA WMAKmm Weu .... lemma .. Uff-SP I UIdg$11 ___gl ___lllIF a WARM 1191 w...ANY COMBINATION THEREOF. BLOWING SNOW: ALL REPORTED BLOWING SNOWS INCLUDING DRIFTING wHrF REPORTED. OLST AND/OR SAND: ALL REPORTED DUST, SAND, BLOWING...T To I To TO ITo To 70 I TH MONTH I NONE ITRACE1I 1 2 3 1 6 1 12 1 286 36 1 48 1 60 1 120 I 12D I MEAS I OBSI II I I I I I I I I ANTS I I EAN

Minitrack on data and knowledge base issues in genomics at the 27th Hawaii International Conference on system sciences

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-05-01

This report is a summary of the proceedings from the Minitrack on Data and Knowledge Base Issues in Genomics at the 27th Hawaii International Conference on System Science, January 4 - 7, 1994. The minitrack was organized by Dong-Guk Shin (University of Connecticut) and Francois Rechenmann (INRIA, France). Support was jointly provided by the NSF, NIH and DOE. The minitrack included, after rigorous review, ten full papers and four extended abstracts in the following five different research subareas of genome informatics: data modeling and management, sequence analysis, graphical user interface, interoperation in a heterogenous computing environment, and system integration inmore » a knowledge-based approach.« less

Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals.

PubMed

Gao, Caixia; Ding, Yingying; Zhou, Peng; Feng, Jiaojiao; Qian, Baohua; Lin, Ziyu; Wang, Lili; Wang, Jinhong; Zhao, Chunyan; Li, Xiangyu; Cao, Mingmei; Peng, Heng; Rui, Bing; Pan, Wei

2016-02-26

The overall serological prevalence of EV infections based on ELISA remains unknown. In the present study, the antibody responses against VP1 of the EV-A species (enterovirus 71 (EV71), Coxsackievirus A16 (CA16), Coxsackievirus A5 (CA5) and Coxsackievirus A6 (CA6)), of the EV-B species (Coxsackievirus B3 (CB3)), and of the EV-C species (Poliovirus 1 (PV1)) were detected and analyzed by a NEIBM (novel evolved immunoglobulin-binding molecule)-based ELISA in Shanghai blood donors. The serological prevalence of anti-CB3 VP1 antibodies was demonstrated to show the highest level, with anti-PV1 VP1 antibodies at the second highest level, and anti-CA5, CA6, CA16 and EV71 VP1 antibodies at a comparatively low level. All reactions were significantly correlated at different levels, which were approximately proportional to their sequence similarities. Antibody responses against EV71 VP1 showed obvious differences with responses against other EV-A viruses. Obvious differences in antibody responses between August 2013 and May 2014 were revealed. These findings are the first to describe the detailed information of the serological prevalence of human antibody responses against the VP1 of EV-A, B and C viruses, and could be helpful for understanding of the ubiquity of EV infections and for identifying an effective approach for seroepidemiological surveillance based on ELISA.

A comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain FY-23 K-B in vitro.

PubMed

Yang, Ting; Li, Hua; Yue, Lei; Song, Xia; Xie, Tianhong; Ma, Shaohui; Meng, Huaqing; Zhang, Ye; He, Xin; Long, Runxiang; Yang, Rong; Luo, Fangyu; Xie, Zhongping; Li, Qihan

2017-10-26

Enterovirus 71 (EV71) is one of the causative agents of hand, foot and mouth disease, which mostly affects infants and children and leads to severe neurological diseases. Vaccination offers the best option for disease control. We have screened the virus strain FY-23 K-B, which is used as an inactivated vaccine strain. An important issue in the development of vaccines is whether they provide cross protection against all other strains. We collected and identified 19 clinical EV71 isolates from mainland China, which all belong to the C4 genotype. We established growth curves of the strains in Vero cells, performed genetic analysis, and evaluated the cross protection efficacy through neutralizing assays using antisera from a rabbit, monkey and adult human immunized with the FY-23 K-B vaccine strain. The antisera showed broad cross protection among the C4 subgroup strains and homotype strain. Neutralizing indexes (NIs) among the isolates and homotype strain of antisera varied between 56.2-1995.3 for rabbit, 17.8-42,169.7 for monkey and 31.6-17,782.8 for human, whereas NIs against Coxsackievirus A16 or other enteroviruses were below 10. These results suggested that FY-23 K-B used as an antigen could elicit broad spectrum neutralizing antibodies with cross protective efficacy among C4 genotype strains.

Phylodynamics of Enterovirus A71-Associated Hand, Foot, and Mouth Disease in Viet Nam.

PubMed

Geoghegan, Jemma L; Tan, Le Van; Kühnert, Denise; Halpin, Rebecca A; Lin, Xudong; Simenauer, Ari; Akopov, Asmik; Das, Suman R; Stockwell, Timothy B; Shrivastava, Susmita; Ngoc, Nghiem My; Uyen, Le Thi Tam; Tuyen, Nguyen Thi Kim; Thanh, Tran Tan; Hang, Vu Thi Ty; Qui, Phan Tu; Hung, Nguyen Thanh; Khanh, Truong Huu; Thinh, Le Quoc; Nhan, Le Nguyen Thanh; Van, Hoang Minh Tu; Viet, Do Chau; Tuan, Ha Manh; Viet, Ho Lu; Hien, Tran Tinh; Chau, Nguyen Van Vinh; Thwaites, Guy; Grenfell, Bryan T; Stadler, Tanja; Wentworth, David E; Holmes, Edward C; Van Doorn, H Rogier

2015-09-01

Enterovirus A71 (EV-A71) is a major cause of hand, foot, and mouth disease (HFMD) and is particularly prevalent in parts of Southeast Asia, affecting thousands of children and infants each year. Revealing the evolutionary and epidemiological dynamics of EV-A71 through time and space is central to understanding its outbreak potential. We generated the full genome sequences of 200 EV-A71 strains sampled from various locations in Viet Nam between 2011 and 2013 and used these sequence data to determine the evolutionary history and phylodynamics of EV-A71 in Viet Nam, providing estimates of the effective reproduction number (Re) of the infection through time. In addition, we described the phylogeography of EV-A71 throughout Southeast Asia, documenting patterns of viral gene flow. Accordingly, our analysis reveals that a rapid genogroup switch from C4 to B5 likely took place during 2012 in Viet Nam. We show that the Re of subgenogroup C4 decreased during the time frame of sampling, whereas that of B5 increased and remained >1 at the end of 2013, corresponding to a rise in B5 prevalence. Our study reveals that the subgenogroup B5 virus that emerged into Viet Nam is closely related to variants that were responsible for large epidemics in Malaysia and Taiwan and therefore extends our knowledge regarding its associated area of endemicity. Subgenogroup B5 evidently has the potential to cause more widespread outbreaks across Southeast Asia. EV-A71 is one of many viruses that cause HFMD, a common syndrome that largely affects infants and children. HFMD usually causes only mild illness with no long-term consequences. Occasionally, however, severe infection may arise, especially in very young children, causing neurological complications and even death. EV-A71 is highly contagious and is associated with the most severe HFMD cases, with large and frequent epidemics of the virus recorded worldwide. Although major advances have been made in the development of a potential EV-A71 vaccine, there is no current prevention and little is known about the patterns and dynamics of EV-A71 spread. In this study, we utilize full-length genome sequence data obtained from HFMD patients in Viet Nam, a geographical region where the disease has been endemic since 2003, to characterize the phylodynamics of this important emerging virus. Copyright © 2015 Geoghegan et al.

Phylodynamics of Enterovirus A71-Associated Hand, Foot, and Mouth Disease in Viet Nam

PubMed Central

Kühnert, Denise; Halpin, Rebecca A.; Lin, Xudong; Simenauer, Ari; Akopov, Asmik; Das, Suman R.; Stockwell, Timothy B.; Shrivastava, Susmita; Ngoc, Nghiem My; Uyen, Le Thi Tam; Tuyen, Nguyen Thi Kim; Thanh, Tran Tan; Hang, Vu Thi Ty; Qui, Phan Tu; Hung, Nguyen Thanh; Khanh, Truong Huu; Thinh, Le Quoc; Nhan, Le Nguyen Thanh; Van, Hoang Minh Tu; Viet, Do Chau; Tuan, Ha Manh; Viet, Ho Lu; Hien, Tran Tinh; Chau, Nguyen Van Vinh; Thwaites, Guy; Grenfell, Bryan T.; Stadler, Tanja; Wentworth, David E.; Holmes, Edward C.; Van Doorn, H. Rogier

2015-01-01

ABSTRACT Enterovirus A71 (EV-A71) is a major cause of hand, foot, and mouth disease (HFMD) and is particularly prevalent in parts of Southeast Asia, affecting thousands of children and infants each year. Revealing the evolutionary and epidemiological dynamics of EV-A71 through time and space is central to understanding its outbreak potential. We generated the full genome sequences of 200 EV-A71 strains sampled from various locations in Viet Nam between 2011 and 2013 and used these sequence data to determine the evolutionary history and phylodynamics of EV-A71 in Viet Nam, providing estimates of the effective reproduction number (Re) of the infection through time. In addition, we described the phylogeography of EV-A71 throughout Southeast Asia, documenting patterns of viral gene flow. Accordingly, our analysis reveals that a rapid genogroup switch from C4 to B5 likely took place during 2012 in Viet Nam. We show that the Re of subgenogroup C4 decreased during the time frame of sampling, whereas that of B5 increased and remained >1 at the end of 2013, corresponding to a rise in B5 prevalence. Our study reveals that the subgenogroup B5 virus that emerged into Viet Nam is closely related to variants that were responsible for large epidemics in Malaysia and Taiwan and therefore extends our knowledge regarding its associated area of endemicity. Subgenogroup B5 evidently has the potential to cause more widespread outbreaks across Southeast Asia. IMPORTANCE EV-A71 is one of many viruses that cause HFMD, a common syndrome that largely affects infants and children. HFMD usually causes only mild illness with no long-term consequences. Occasionally, however, severe infection may arise, especially in very young children, causing neurological complications and even death. EV-A71 is highly contagious and is associated with the most severe HFMD cases, with large and frequent epidemics of the virus recorded worldwide. Although major advances have been made in the development of a potential EV-A71 vaccine, there is no current prevention and little is known about the patterns and dynamics of EV-A71 spread. In this study, we utilize full-length genome sequence data obtained from HFMD patients in Viet Nam, a geographical region where the disease has been endemic since 2003, to characterize the phylodynamics of this important emerging virus. PMID:26085170

Immunodominant IgM and IgG Epitopes Recognized by Antibodies Induced in Enterovirus A71-Associated Hand, Foot and Mouth Disease Patients

PubMed Central

Aw-Yong, Kam Leng; Sam, I-Ching; Koh, Mia Tuang

2016-01-01

Enterovirus A71 (EV-A71) is one of the main causative agents of hand, foot and mouth disease (HFMD). Unlike other enteroviruses that cause HFMD, EV-A71 is more frequently associated with severe neurological complications and fatality. To date, no effective licensed antivirals are available to combat EV-A71 infection. Little is known about the immunogenicity of viral non-structural proteins in humans. Previous studies have mainly focused on characterization of epitopes of EV-A71 structural proteins by using immunized animal antisera. In this study, we have characterized human antibody responses against the structural and non-structural proteins of EV-A71. Each viral protein was cloned and expressed in either bacterial or mammalian systems, and tested with antisera by western blot. Results revealed that all structural proteins (VP1-4), and non-structural proteins 2A, 3C and 3D were targets of EV-A71 IgM, whereas EV-A71 IgG recognized all the structural and non-structural proteins. Sixty three synthetic peptides predicted to be immunogenic in silico were synthesized and used for the characterization of EV-A71 linear B-cell epitopes. In total, we identified 22 IgM and four IgG dominant epitopes. Synthetic peptide PEP27, corresponding to residues 142–156 of VP1, was identified as the EV-A71 IgM-specific immunodominant epitope. PEP23, mapped to VP1 41–55, was recognized as the EV-A71 IgG cross-reactive immunodominant epitope. The structural protein VP1 is the major immunodominant site targeted by anti-EV-A71 IgM and IgG antibodies, but epitopes against non-structural proteins were also detected. These data provide new understanding of the immune response to EV-A71 infection, which benefits the development of diagnostic tools, potential therapeutics and subunit vaccine candidates. PMID:27806091

Inhibition of enterovirus 71 (EV-71) infections by a novel antiviral peptide derived from EV-71 capsid protein VP1.

PubMed

Tan, Chee Wah; Chan, Yoke Fun; Sim, Kooi Mow; Tan, Eng Lee; Poh, Chit Laa

2012-01-01

Enterovirus 71 (EV-71) is the main causative agent of hand, foot and mouth disease (HFMD). In recent years, EV-71 infections were reported to cause high fatalities and severe neurological complications in Asia. Currently, no effective antiviral or vaccine is available to treat or prevent EV-71 infection. In this study, we have discovered a synthetic peptide which could be developed as a potential antiviral for inhibition of EV-71. Ninety five synthetic peptides (15-mers) overlapping the entire EV-71 capsid protein, VP1, were chemically synthesized and tested for antiviral properties against EV-71 in human Rhabdomyosarcoma (RD) cells. One peptide, SP40, was found to significantly reduce cytopathic effects of all representative EV-71 strains from genotypes A, B and C tested, with IC(50) values ranging from 6-9.3 µM in RD cells. The in vitro inhibitory effect of SP40 exhibited a dose dependent concentration corresponding to a decrease in infectious viral particles, total viral RNA and the levels of VP1 protein. The antiviral activity of SP40 peptide was not restricted to a specific cell line as inhibition of EV-71 was observed in RD, HeLa, HT-29 and Vero cells. Besides inhibition of EV-71, it also had antiviral activities against CV-A16 and poliovirus type 1 in cell culture. Mechanism of action studies suggested that the SP40 peptide was not virucidal but was able to block viral attachment to the RD cells. Substitutions of arginine and lysine residues with alanine in the SP40 peptide at positions R3A, R4A, K5A and R13A were found to significantly decrease antiviral activities, implying the importance of positively charged amino acids for the antiviral activities. The data demonstrated the potential and feasibility of SP40 as a broad spectrum antiviral agent against EV-71.

48 CFR 301.603-71 - Waivers to warrant standards.

Code of Federal Regulations, 2011 CFR

2011-10-01

... of a warrant to an individual who does not fully meet the FAC-C or HHS SAC certification program requirements. Contracting activities shall provide any request for a waiver of the FAC-C program requirements...

48 CFR 301.603-71 - Waivers to warrant standards.

Code of Federal Regulations, 2014 CFR

2014-10-01

... of a warrant to an individual who does not fully meet the FAC-C or HHS SAC certification program requirements. Contracting activities shall provide any request for a waiver of the FAC-C program requirements...

48 CFR 301.603-71 - Waivers to warrant standards.

Code of Federal Regulations, 2013 CFR

2013-10-01

... of a warrant to an individual who does not fully meet the FAC-C or HHS SAC certification program requirements. Contracting activities shall provide any request for a waiver of the FAC-C program requirements...