Flaw Tolerance in Lap Shear Brazed Joints. Part 1

NASA Technical Reports Server (NTRS)

Flom, Yury; Wang, Li-Qin

2003-01-01

Furnace brazing is a joining process used in the aerospace and other industries to produce strong permanent and hermetic structural joints. As in any joining process, brazed joints have various imperfections and defects. At the present time, our understanding of the influence of the internal defects on the strength of the brazed joints is not adequate. The goal of this 3-part investigation is to better understand the properties and failure mechanisms of the brazed joints containing defects. This study focuses on the behavior of the brazed lap shear joints because of their importance in manufacturing aerospace structures. In Part 1, an average shear strength capability and failure modes of the single lap joints are explored. Stainless steel specimens brazed with pure silver are tested in accordance with the AWS C3.2 standard. Comparison of the failure loads and the ultimate shear strength with the Finite Element Analysis (FEA) of the same specimens as a function of the overlap widths shows excellent correlation between the experimental and calculated values for the defect-free lap joints. A damage zone criterion is shown to work quite well in understanding the failure of the braze joints. In Part 2, the findings of the Part 1 will be verified on the larger test specimens. Also, various flaws will be introduced in the test specimens to simulate lack of braze coverage in the lap joints. Mechanical testing and FEA will be performed on these joints to verify that behavior of the flawed ductile lap joints is similar to joints with a reduced braze area. Finally, in Part 3, the results obtained in Parts 1 and 2 will be applied to the actual brazed structure to evaluate the load-carrying capability of a structural lap joint containing discontinuities. In addition, a simplified engineering procedure will be offered for the laboratory testing of the lap shear specimens.

Mechanical properties of dissimilar metal joints composed of DP 980 steel and AA 7075-T6

DOE PAGES

Squires, Lile; Lim, Yong Chae; Miles, Michael; ...

2015-03-18

In this study, a solid state joining process, called friction bit joining, was used to spot weld aluminium alloy 7075-T6 to dual phase 980 steel. Lap shear failure loads for specimens without adhesive averaged ~10kN, while cross-tension specimens averaged 2·8 kN. Addition of adhesive with a thickness up to 500 μm provided a gain of ~50% to lap shear failure loads, while a much thinner layer of adhesive increased cross-tension failure loads by 20%. Microstructures of the welds were martensitic, but the hardness of the joining bit portion was greater than that of the DP 980, owing to its highermore » alloy content. Softening in the heat affected zone of a welded joint appeared to be relatively small, though it was enough to cause nugget pullout failures in some lap shear tension specimens. Finally, other failures in lap shear tension were interfacial, while all of the failures in cross-tension were interfacial.« less

Sequential lineup laps and eyewitness accuracy.

PubMed

Steblay, Nancy K; Dietrich, Hannah L; Ryan, Shannon L; Raczynski, Jeanette L; James, Kali A

2011-08-01

Police practice of double-blind sequential lineups prompts a question about the efficacy of repeated viewings (laps) of the sequential lineup. Two laboratory experiments confirmed the presence of a sequential lap effect: an increase in witness lineup picks from first to second lap, when the culprit was a stranger. The second lap produced more errors than correct identifications. In Experiment 2, lineup diagnosticity was significantly higher for sequential lineup procedures that employed a single versus double laps. Witnesses who elected to view a second lap made significantly more errors than witnesses who chose to stop after one lap or those who were required to view two laps. Witnesses with prior exposure to the culprit did not exhibit a sequential lap effect.

Material characterization of structural adhesives in the lap shear mode

NASA Technical Reports Server (NTRS)

Sancaktar, E.; Schenck, S. C.

1983-01-01

A general method for characterizing structual adhesives in the bonded lap shear mode is proposed. Two approaches in the form of semiempirical and theoretical approaches are used. The semiempirical approach includes Ludwik's and Zhurkov's equations to describe respectively, the failure stresses in the constant strain rate and constant stress loading modes with the inclusion of the temperature effects. The theoretical approach is used to describe adhesive shear stress-strain behavior with the use of viscoelastic or nonlinear elastic constitutive equations. Two different model adhesives are used in the single lap shear mode with titanium adherends. These adhesives (one of which was developed at NASA Langley Research Center) are currently considered by NASA for possible aerospace applications. Use of different model adhesives helps in assessment of the generality of the method.

Analysis of delamination related fracture processes in composites

NASA Technical Reports Server (NTRS)

Armanios, Erian A.

1992-01-01

This is a final report that summarizes the results achieved under this grant. The first major accomplishment is the development of the sublaminate modeling approach and shear deformation theory. The sublaminate approach allows the flexibility of considering one ply or groups of plies as a single laminated unit with effective properties. This approach is valid when the characteristic length of the response is small compared to the sublaminate thickness. The sublaminate approach was validated comparing its predictions with a finite element solution. A shear deformation theory represents an optimum compromise between accuracy and computational effort in delamination analysis of laminated composites. This conclusion was reached by applying several theories with increasing level of complexity to the prediction of interlaminar stresses and strain energy release rate in a double cracked-lap-shear configuration.

Hybrid Microcircuit Rework Procedures Evaluation.

DTIC Science & Technology

1980-08-01

replacement Task III. Polymer Attachment Rework (a) Die replacement (b) Substrate replacement Task IV. Interconnection Rework (a) Gold and aluminum ...the following conclusions: (a) Lap Shear Strength The shear strength ( aluminum to aluminum ) values ranged from a high of 4000 psi (for Ablefilm 550...bonded specimens ( aluminum to aluminum ) to 150°C for 10, 20 and 35 days prior to testing. No significant degradation of lap shear strength of any of the

Stress relaxation and mechanical properties of RL-1973 and PD-200-16 silicone resin sponge materials

NASA Technical Reports Server (NTRS)

Saylak, D.; Noel, J. S.; Ham, J. S.; Mccoy, R.

1975-01-01

Stress relaxation tests were conducted by loading specimens in double-lap shear to a preselected strain level and monitoring the decay of stress with time. The stress relaxation response characteristics were measured over a temperature range of 100 to 300 K and four strain levels. It is concluded that only a slight amount of stress relaxation was observed, and the stiffness increased approximately two orders of magnitude over the range of temperatures.

Design Optimization and Analysis of a Composite Honeycomb Intertank

NASA Technical Reports Server (NTRS)

Finckenor, Jeff; Spurrier, Mile

1999-01-01

Intertanks, the structure between tanks of launch vehicles, are prime candidates for weight reduction of rockets. This paper discusses the optimization and detailed follow up analysis and testing of a 96 in. diameter, 77 in. tall intertank. The structure has composite face sheets with an aluminum honeycomb core. The ends taper to a thick built up laminate for a double lap bolted splice joint interface. It is made in 8 full length panels joined with bonded double lap joints. The nominal load is 4000 lb/in. Optimization is by Genetic Algorithm and minimizes weight by varying core thickness, number and orientation of acreage and buildup plies, and the size, number and spacing of bolts. A variety of design cases were run with populations up to 2000 and chromosomes as long as 150 bits. Constraints were buckling; face stresses (normal, shear, wrinkling and dimpling); bolt stress; and bolt hole stresses (bearing, net tension, wedge splitting, shear out and tension/shear out). Analysis is by a combination of elasticity solutions and empirical data. After optimization, a series of coupon tests were performed in conjunction with a rigorous analysis involving a variety of finite element models. This analysis and testing resulted in several small changes to the optimized design. The equation used for hole bearing strength was found to be inadequate, resulting in thicker ends. The core thickness increased 0.05", and potting compound was added in the taper to strengthen the facesheet bond. The intertank has undergone a 250,000 lb limit load test and been mated with a composite liquid hydrogen tank. The tank/intertank unit is being installed in a test stand where it will see 200 thermal/load cycles. Afterwards the intertank will be demated and loaded in compression to failure.

Effect of Different Connection Modes on Bolt Structural Properties of TC4 Alloy in Selective Laser Melting

NASA Astrophysics Data System (ADS)

Li, Xiaodan; Huang, Shuangjun; Xu, Liang; Hui, Li; Zhou, Song

2017-12-01

The bolt structural properties of selective laser melted (SLM) samples produced from TC4 powder metal has been investigated. Two different connection molds relative to single lap joint and bilateral lap joint as well as two different state of surface quality were considered. Samples and test procedures were designed in accordance with HB 5143 and HB 5287 standard. The results show that there is a strong influence of connection molds on the dynamic behavior of SLM produced TC4. The mechanical properties of bilateral lap joint are better than those of the single lap joint. Meanwhile the fatigue performance of the bilateral lap joint is much stronger than that of the single lap joint which it is a symmetrical structure of the two-shear test on both sides of the force evenly, while the single lap joint is a single shear sample of the uneven force. There are two kinds of fracture form most of which are broken in the first row of screw and a small part in the middle of the connecting plate.

FRICTION STIR LAP WELDING OF ALUMINUM - POLYMER USING SCRIBE TECHNOLOGY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Upadhyay, Piyush; Hovanski, Yuri; Fifield, Leonard S.

2015-02-16

Friction Stir Scribe (FSS) technology is a relatively new variant of Friction Stir Welding (FSW) which enables lap joining of dissimilar material with very different melting points and different high temperature flow behaviors. The cutter scribe attached at the tip of FSW tool pin effectively cuts the high melting point material such that a mechanically interlocking feature is created between the dissimilar materials. The geometric shape of this interlocking feature determines the shear strength attained by the lap joint. This work presents first use of scribe technology in joining polymers to aluminum alloy. Details of the several runs of scribemore » welding performed in lap joining of ~3.175mm thick polymers including HDPE, filled and unfilled Nylon 66 to 2mm thick AA5182 are presented. The effect of scribe geometry and length on weld interlocking features is presented along with lap shear strength evaluations.« less

Shear Pressed Aligned Carbon Nanotubes and their use as Composite and Adhesive Interlayers

NASA Astrophysics Data System (ADS)

Stahl, James Joseph, III

The following studies utilize shearing force to consolidate and re-orient multi-walled carbon nanotubes (MWCNT) into a shear pressed sheet (SPS) preform. Carbon nanotube (CNT) array growth and shear pressing angle are studied to improve the quality of SPSs. Heat assisted vacuum infusion is used to form a nano-composite from the SPS preform, and mechanical properties are characterized and compared between non-functionalized and functionalized nano-composite tensile specimens. A novel functionalization technique is applied which rinses SPSs with an acidic wet chemical oxidation treatment of H2SO4 and KMnO4 in order to add sidewall carboxyl groups to the CNTs. This is shown to impart hydrophilicity to the SPS and improves composite modulus by 62%, strain-to-failure 42% and failure stress 113%. Composite laminates and joints are vulnerable to shearing forces which cause delamination in the former and failure in the latter. Damage is initiated and propagated at defects and free edges often due to high peel stress, which is much higher than the shear stress and functions as a tensile opening of the joint just as in Mode I delamination failure of laminate composites. In order to resist failure it is necessary to improve the strain-to-failure of the interphase where a crack propagates without sacrificing strength or modulus of the material, thus toughening the material without impacting the rigidity of the composite. Due to the similarity between peel stress/strain and Mode I delamination, the initiation fracture toughness of a double cantilever beam (DCB) test should provide a good indication of peel toughness at a joint free edge. Many studies have explored the possibility of improving Mode I fracture toughness (G IC) of a composite through locally incorporating a tough material into the interlaminar interphase; this material is termed an interleaf. Common interleaf categories are toughened adhesive, disperse particle, disperse fiber, short fiber nonwoven, and continuous fiber nonwoven. A SPS falls into a short fiber nonwoven and is studied as a non-infused, infused, and infused functionalized interleaf in unidirectional carbon fiber composites for GIC improvement over non-interleaved samples. As with traditional interleaving studies it is possible to decrease delamination fracture toughness as well as increase, and the reasons for either are not always clear. While the SPS interleaves are promising to resist delamination, the scatter of the results make it an unreliable method of improvement. While these studies showed significant variability in effect of the interleaf, given the correct morphology of the SPS and precise measurement during the DCB testing it is possible to improve fracture toughness significantly with all SPS interleaves. A unique fabrication method is used to incorporate the SPS interleaves into lap joint and double strap joint geometries using a prepreg lay-up fabrication similar to forming the DCB specimens. This allowed study of the use of the SPS interleaf as an adhesive layer without the need to develop a SPS adhesive film that would not fail prematurely due to poor adhesion to cured composite panels. Results showed that improvement in GIC is not directly translated into improvement in joint strength. Lap joints showed a higher relationship between GIC than double strap joints likely due to the specimen geometry that results in the adhesive layer of lap joints failing in tension rather than shear.

A fundamental study on the structural integrity of magnesium alloys joined by friction stir welding

NASA Astrophysics Data System (ADS)

Rao, Harish Mangebettu

The goal of this research is to study the factors that influence the physical and mechanical properties of lap-shear joints produced using friction stir welding. This study focuses on understanding the effect of tool geometry and weld process parameters including the tool rotation rate, tool plunge depth and dwell time on the mechanical performance of similar magnesium alloy and dissimilar magnesium to aluminum alloy weld joints. A variety of experimental activities were conducted including tensile and fatigue testing, fracture surface and failure analysis, microstructure characterization, hardness measurements and chemical composition analysis. An investigation on the effect of weld process conditions in friction stir spot welding of magnesium to magnesium produced in a manner that had a large effective sheet thickness and smaller interfacial hook height exhibited superior weld strength. Furthermore, in fatigue testing of friction stir spot welded of magnesium to magnesium alloy, lap-shear welds produced using a triangular tool pin profile exhibited better fatigue life properties compared to lap-shear welds produced using a cylindrical tool pin profile. In friction stir spot welding of dissimilar magnesium to aluminum, formation of intermetallic compounds in the stir zone of the weld had a dominant effect on the weld strength. Lap-shear dissimilar welds with good material mixture and discontinues intermetallic compounds in the stir zone exhibited superior weld strength compared to lap-shear dissimilar welds with continuous formation of intermetallic compounds in the stir zone. The weld structural geometry like the interfacial hook, hook orientation and bond width also played a major role in influencing the weld strength of the dissimilar lap-shear friction stir spot welds. A wide scatter in fatigue test results was observed in friction stir linear welds of aluminum to magnesium alloys. Different modes of failure were observed under fatigue loading including crack propagation into the top sheet, into the bottom sheet, and interfacial separation. Investigation of the tested welds revealed that the voids in the weld nugget reduced the weld strength, resulting in lower fatigue life. A thin layer of IMCs formed along the faying surface which accelerated the fatigue failure.

Strain measurements in composite bolted-joint specimens

NASA Technical Reports Server (NTRS)

Hyer, M. W.; Lightfoot, M. C.; Perry, J. C.

1979-01-01

Strain data from a series of bolted joint tests is presented. Double lap, double hole, double lap, single hole, and open hole tensile specimens were tested and the strain gage locations, load strain responses, and load axial displacement responses are presented. The open hole specimens were gaged to determine strain concentration factors. The double lap, double hole specimens were gaged to determine the uniformity of the strain in the joint and the amount of load transferred past the first bolt. The measurements indicated roughly half the load passed the first bolt to be reacted by the second bolt.

Tensile Shear Properties of the Friction Stir Lap Welded Joints and Material Flow Mechanism Under Pulsatile Revolutions

NASA Astrophysics Data System (ADS)

Hu, Yanying; Liu, Huijie; Du, Shuaishuai

2018-06-01

The aim of the present article is to offer insight into the effects of pin profiles on interface defects, tensile shear properties, microstructures, and the material flow of friction stir lap welded joints. The results indicate that, compared to the lap joints welded by the single threaded plane pin, the three-plane threaded pin, and the triangle threaded pin, the lap joint obtained by the conventional conical threaded pin is characterized by the minimum interface defect. The alternate threads and planes on the pin provide periodical stress, leading to pulsatile material flow patterns. Under the effect of pulsatile revolutions, an asymmetrical flow field is formed around the tool. The threads on the pin force the surrounding material to flow downward. The planes cannot only promote the horizontal flow of the material by scraping, but also provide extra space for the material vertical flow. A heuristic model is established to describe the material flow mechanism during friction stir lap welding under the effect of pulsatile revolutions.

Defect features, texture and mechanical properties of friction stir welded lap joints of 2A97 Al-Li alloy thin sheets

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Haiyan

1.4 mm 2A97 Al-Li alloy thin sheets were welded by friction stir lap welding using the stirring tools with different pin length at different rotational speeds. The influence of pin length and rotational speed on the defect features and mechanical properties of lap joints were investigated in detail. Microstructure observation shows that the hook defect geometry and size mainly varies with the pin length instead of the rotational speed. The size of hook defects on both the advancing side (AS) and the retreating side (RS) increased with increasing the pin length, leading to the effective sheet thickness decreased accordingly. Electronmore » backscatter diffraction analysis reveals that the weld zones, especially the nugget zone (NZ), have the much lower texture intensity than the base metal. Some new texture components are formed in the thermo-mechanical affected zone (TMAZ) and the NZ of joint. Lap shear test results show that the failure load of joints generally decreases with increasing the pin length and the rotational speed. The joints failed during the lap shear tests at three locations: the lap interface, the RS of the top sheet and the AS of the bottom sheet. The fracture locations are mainly determined by the hook defects. - Highlights: • Hook defect size mainly varies with the pin length of stirring tool. • The proportion of LAGBs and substructured grains increases from NZ to TMAZ. • Weld zones, especially the NZ, have the much lower texture intensity than the BM. • Lap shear failure load and fracture location of joints is relative to the hook defects.« less

Fatigue crack growth in 2024-T3 aluminum under tensile and transverse shear stresses

NASA Technical Reports Server (NTRS)

Viz, Mark J.; Zehnder, Alan T.

1994-01-01

The influence of transverse shear stresses on the fatigue crack growth rate in thin 2024-T3 aluminum alloy sheets is investigated experimentally. The tests are performed on double-edge cracked sheets in cyclic tensile and torsional loading. This loading generates crack tip stress intensity factors in the same ratio as the values computed for a crack lying along a lap joint in a pressurized aircraft fuselage. The relevant fracture mechanics of cracks in thin plates along with the details of the geometrically nonlinear finite element analyses used for the test specimen calibration are developed and discussed. Preliminary fatigue crack growth data correlated using the fully coupled stress intensity factor calibration are presented and compared with fatigue crack growth data from pure delta K(sub I)fatigue tests.

SEM/XPS analysis of fractured adhesively bonded graphite fibre surface resin-rich/graphite fibre composites

NASA Technical Reports Server (NTRS)

Devilbiss, T. A.; Wightman, J. P.; Progar, D. J.

1988-01-01

Samples of graphite fiber-reinforced polyimide were fabricated allowing the resin to accumulate at the composite surface. These surface resin-rich composites were then bonded together and tested for lap shear strength both before and after thermal aging. Lap shear strength did not appear to show a significant improvement over that previously recorded for resin-poor samples and was shown to decrease with increasing aging time and temperature.

Friction stir welding tool and process for welding dissimilar materials

DOEpatents

Hovanski, Yuri; Grant, Glenn J; Jana, Saumyadeep; Mattlin, Karl F

2013-05-07

A friction stir welding tool and process for lap welding dissimilar materials are detailed. The invention includes a cutter scribe that penetrates and extrudes a first material of a lap weld stack to a preselected depth and further cuts a second material to provide a beneficial geometry defined by a plurality of mechanically interlocking features. The tool backfills the interlocking features generating a lap weld across the length of the interface between the dissimilar materials that enhances the shear strength of the lap weld.

Lap Shear Testing of Candidate Radiator Panel Adhesives

NASA Technical Reports Server (NTRS)

Ellis, David; Briggs, Maxwell; McGowan, Randy

2013-01-01

During testing of a subscale radiator section used to develop manufacturing techniques for a full-scale radiator panel, the adhesive bonds between the titanium heat pipes and the aluminum face sheets failed during installation and operation. Analysis revealed that the thermal expansion mismatch between the two metals resulted in relatively large shear stresses being developed even when operating the radiator at moderate temperatures. Lap shear testing of the adhesive used in the original joints demonstrated that the two-part epoxy adhesive fell far short of the strength required. A literature review resulted in several candidate adhesives being selected for lap shear joint testing at room temperature and 398 K, the nominal radiator operating temperature. The results showed that two-part epoxies cured at room and elevated temperatures generally did not perform well. Epoxy film adhesives cured at elevated temperatures, on the other hand, did very well with most being sufficiently strong to cause yielding in the titanium sheet used for the joints. The use of an epoxy primer generally improved the strength of the joint. Based upon these results, a new adhesive was selected for the second subscale radiator section.

Nonlinear Analysis of Bonded Composite Tubular Lap Joints

NASA Technical Reports Server (NTRS)

Oterkus, E.; Madenci, E.; Smeltzer, S. S., III; Ambur, D. R.

2005-01-01

The present study describes a semi-analytical solution method for predicting the geometrically nonlinear response of a bonded composite tubular single-lap joint subjected to general loading conditions. The transverse shear and normal stresses in the adhesive as well as membrane stress resultants and bending moments in the adherends are determined using this method. The method utilizes the principle of virtual work in conjunction with nonlinear thin-shell theory to model the adherends and a cylindrical shear lag model to represent the kinematics of the thin adhesive layer between the adherends. The kinematic boundary conditions are imposed by employing the Lagrange multiplier method. In the solution procedure, the displacement components for the tubular joint are approximated in terms of non-periodic and periodic B-Spline functions in the longitudinal and circumferential directions, respectively. The approach presented herein represents a rapid-solution alternative to the finite element method. The solution method was validated by comparison against a previously considered tubular single-lap joint. The steep variation of both peeling and shearing stresses near the adhesive edges was successfully captured. The applicability of the present method was also demonstrated by considering tubular bonded lap-joints subjected to pure bending and torsion.

A Fundamental Approach to Adhesion: Synthesis, Surface Analysis, Thermodynamics and Mechanics.

DTIC Science & Technology

1978-02-01

Polyphenylquinoxaline LSS - Lap Shear Strength Pasa-Jell - Commercial acid etch (See p. 15 ) Turco - Commercial base etch (See p. 17 ) CTBN - Carboxyl-Terminated...solvent- cast films or powders. SEM/EDAX results were obtained from the fracture surfaces of lap-shear tested specimens. Epoxy and two epoxy/ CTBN bulk...A - - 24 CTBN 1300X8 (Goodrich carboxyl- 5 5 terminated butadiene-acrylonitrile) Piperidine 5 5 5 L9 III. RESULTS AND DISCUSS10N A. Titanium 6-4 (SEM

The Effect of Different Chemical Surface Treatments of Denture Teeth on Shear Bond Strength: A Comparative Study

PubMed Central

Palekar, Umesh; Awinashe, Vaibav; Mishra, Sunil Kumar; Kawadkar, Abhishek; Rahangdale, Tripti

2014-01-01

Background: The development of better cross linked acrylic resin teeth has solved the problems related to wearing and discoloration of acrylic teeth. The same cross linking at ridge lap region acts as a double edge sword as it weakens the bond between denture base and tooth. Aim of Study: The purpose of study was to evaluate the effect of surface treatment on the bond strength of resin teeth to denture base resin using monomethyl methacrylate monomer and dichloromethane with no surface treatment acting as control. Settings and Design:Denture base cylinder samples in wax (n=180) were made with maxillary central incisor attached at 450 (JIST 6506). These samples were randomly and equally divided into three groups of 60 each. These specimens were then flasked, dewaxed as per the standard protocol. Materials and Methods: Before acrylization, ridge lap area was treated as follows: Group A- no surface treatment act as control, Group B treated with monomethyl methacrylate monomer, Group C treated with dichloromethane. Digitally controlled acryliser was used for acrylization as per manufacturer’s instructions and shear bond strength was tested on Universal Testing Machine (Servo Hydraulic, 50kN High Strain, BISS Research). Statistical Analysis used: Result was statistically analyzed with One-way analysis of variance (ANOVA) and Post-hoc ANOVA Tukey’s HSD test at 5% level of significance. Results: The application of dichloromethane showed increased bond strength between cross linked acrylic resin teeth and heat cure denture base resin followed by monomethyl methacrylate monomer and control group. Conclusion: The application of dichloromethane on the ridge lap surface of the resin teeth before packing of the dough into the mold significantly increased the bond strength between cross linked acrylic resin teeth and heat cure denture base resin. PMID:25121057

System integration and demonstration of adhesive bonded high temperature aluminum alloys for aerospace structure, phase 2

NASA Technical Reports Server (NTRS)

Falcone, Anthony; Laakso, John H.

1993-01-01

Adhesive bonding materials and processes were evaluated for assembly of future high-temperature aluminum alloy structural components such as may be used in high-speed civil transport aircraft and space launch vehicles. A number of candidate high-temperature adhesives were selected and screening tests were conducted using single lap shear specimens. The selected adhesives were then used to bond sandwich (titanium core) test specimens, adhesive toughness test specimens, and isothermally aged lap shear specimens. Moderate-to-high lap shear strengths were obtained from bonded high-temperature aluminum and silicon carbide particulate-reinforced (SiC(sub p)) aluminum specimens. Shear strengths typically exceeded 3500 to 4000 lb/in(sup 2) and flatwise tensile strengths exceeded 750 lb/in(sup 2) even at elevated temperatures (300 F) using a bismaleimide adhesive. All faceskin-to-core bonds displayed excellent tear strength. The existing production phosphoric acid anodize surface preparation process developed at Boeing was used, and gave good performance with all of the aluminum and silicon carbide particulate-reinforced aluminum alloys investigated. The results of this program support using bonded assemblies of high-temperature aluminum components in applications where bonding is often used (e.g., secondary structures and tear stoppers).

Investigation of defect rate of lap laser welding of stainless steel railway vehicles car body

NASA Astrophysics Data System (ADS)

Wang, Hongxiao

2015-02-01

In order to resolve the disadvantages such as poor appearance quality, poor tightness, low efficiency of resistance spot welding of stainless steel rail vehicles, partial penetration lap laser welding process was investigated widely. But due to the limitation of processing technology, there will be local incomplete fusion in the lap laser welding seam. Defect rate is the ratio of the local incomplete fusion length to the weld seam length. The tensile shear strength under different defect rate and its effect on the car body static strength are not clear. It is necessary to find the biggest defect rate by numerical analysis of effects of different defect rates on the laser welding stainless steel rail vehicle body structure strength ,and tests of laser welding shear tensile strength.

An EMAT-based shear horizontal (SH) wave technique for adhesive bond inspection

NASA Astrophysics Data System (ADS)

Arun, K.; Dhayalan, R.; Balasubramaniam, Krishnan; Maxfield, Bruce; Peres, Patrick; Barnoncel, David

2012-05-01

The evaluation of adhesively bonded structures has been a challenge over the several decades that these structures have been used. Applications within the aerospace industry often call for particularly high performance adhesive bonds. Several techniques have been proposed for the detection of disbonds and cohesive weakness but a reliable NDE method for detecting interfacial weakness (also sometimes called a kissing bond) has been elusive. Different techniques, including ultrasonic, thermal imaging and shearographic methods, have been proposed; all have had some degree of success. In particular, ultrasonic methods, including those based upon shear and guided waves, have been explored for the assessment of interfacial bond quality. Since 3-D guided shear horizontal (SH) waves in plates have predominantly shear displacement at the plate surfaces, we conjectured that SH guided waves should be influenced by interfacial conditions when they propagate between adhesively bonded plates of comparable thickness. This paper describes a new technique based on SH guided waves that propagate within and through a lap joint. Through mechanisms we have yet to fully understand, the propagation of an SH wave through a lap joint gives rise to a reverberation signal that is due to one or more reflections of an SH guided wave mode within that lap joint. Based upon a combination of numerical simulations and measurements, this method shows promise for detecting and classifying interfacial bonds. It is also apparent from our measurements that the SH wave modes can discriminate between adhesive and cohesive bond weakness in both Aluminum-Epoxy-Aluminum and Composite-Epoxy-Composite lap joints. All measurements reported here used periodic permanent magnet (PPM) Electro-Magnetic Acoustic Transducers (EMATs) to generate either or both of the two lowest order SH modes in the plates that comprise the lap joint. This exact configuration has been simulated using finite element (FE) models to describe the SH mode generation, propagation and reception. Of particular interest is that one SH guided wave mode (probably SH0) reverberates within the lap joint. Moreover, in both simulations and measurements, features of this so-called reverberation signal appear to be related to interfacial weakness between the plate (substrate) and the epoxy bond. The results of a hybrid numerical (FE) approach based on using COMSOL to calculate the driving forces within an elastic solid and ABAQUS to propagate the resulting elastic disturbances (waves) within the plates and lap joint are compared with measurements of SH wave generation and reception in lap joint specimens having different interfacial and cohesive bonding conditions.

Fabrication of Titanium Bonded Joint Specimens for High Temperature Testing

NASA Technical Reports Server (NTRS)

Smeltzer, Stanley S., III; Kovach, Michael P.; Hudson, Wanda

2005-01-01

Four sets of adhesively bonded, titanium lap-shear coupon specimens were fabricated for ultimate strength testing according to the ASTM D1002 and D3165 standards. Important features of the fabrication methods, processing details, and lap-shear test results are presented for specimens fabricated using a modified bismaleimide adhesive, EA 9673, on titanium. Surface treatment of the titanium was performed using surface abrasion followed by one of two separate chemical etching processes. Although cure cycle requirements are different among most adhesives, a single surface preparation method was sought as the preferred method for conditioning the titanium specimens prior to bonding and curing. A fabrication process using a combination of low-pressure grit-blasting of the titanium surface followed by anodization with a sodium hydroxide solution applied to the D1002 specimen geometry provided the highest lapshear strengths in the study. Additionally, difficulties documented during the fabrication process of the D3165 specimens along with features of the D3165 geometry were identified as factors that contributed to lower lap-shear strength results for the D3165 specimens as compared to the results for the D1002 specimens.

Study of mechanical joint strength of aluminum alloy 7075-T6 and dual phase steel 980 welded by friction bit joining and weld-bonding under corrosion medium

DOE PAGES

Lim, Yong Chae; Squires, Lile; Pan, Tsung-Yu; ...

2014-12-30

We have employed a unique solid-sate joining process, called friction bit joining (FBJ), to spot weld aluminum alloy (AA) 7075-T6 and dual phase (DP) 980 steel. Static joint strength was studied in the lap shear tension configuration. In addition, weld-bonding (adhesive + FBJ) joints were studied in order to evaluate the ability of adhesive to mitigate the impact of corrosion on joint properties. Accelerated laboratory cyclic corrosion tests were carried out for both FBJ only and weld-bonding joints. Furthermore, the FBJ only joints that emerged from corrosion testing had lap shear failure loads that were significantly lower than freshly preparedmore » joints. However, weld-bonding specimens retained more than 80% of the lap shear failure load of the freshly prepared weld-bonding specimens. Moreover, examination of joint cross sections confirmed that the presence of adhesive in the weld-bonding joints mitigated the effect of the corrosion environment, compared to FBJ only joints.« less

Shear fracture of jointed steel plates of bolted joints under impact load

NASA Astrophysics Data System (ADS)

Daimaruya, M.; Fujiki, H.; Ambarita, H.; Kobayashi, H.; Shin, H.-S.

2013-07-01

The present study is concerned with the development of a fracture criterion for the impact fracture of jointed steel plates of bolted joints used in a car body, which contributes to crash simulations by CAE. We focus our attention on the shear fracture of the jointed steel plates of lap-bolted joints in the suspension of a car under impact load. Members of lap-bolted joints are modelled as a pair of steel plates connected by a bolt. One of the plates is a specimen subjected to plastic deformation and fracture and the other is a jig subjected to elastic deformation only. Three kinds of steel plate specimens are examined, i.e., a common steel plate with a tensile strength of 270 MPa and high tensile strength steel plates of 440 and 590 MPa used for cars. The impact shear test was performed using the split Hopkinson bar technique for tension impact, together with the static test using a universal testing machine INSTRON 5586. The behaviour of the shear stress and deformation up to rupture taking place in the joint was discussed. The obtained results suggest that a stress-based fracture criterion may be developed for the impact fracture of jointed steel plates of a lap-bolted joint.

Study on the Strength of GFRP/Stainless Steel Adhesive Joints Reinforced with Glass Mat

NASA Astrophysics Data System (ADS)

Iwasa, Masaaki

The adhesive strengths of glass fiber reinforced plastics/metal adhesive joints reinforced with glass mat under tensile shear loads and tensile loads were investigated analytically and experimentally. First, the stress singularity parameters of the bonding edges were analyzed by FEM for various types of adhesive joints reinforced with glass mat. The shear stress and normal stress distributions near the bonding edge can be expressed by two stress singularity parameters. Second, tensile shear tests were performed on taper lap joint and taper lap joint reinforced with glass mat and tensile tests were performed on T-type adhesive joint and T-type adhesive joint reinforced with glass mat. The relationships between the loads and the crosshead displacements were measured. We concluded that reinforcing adhesive joints has a greater effect on strength under tensile load than under tensile shear load. The adhesive joints strength reinforced with glass mat can be evaluated by using stress singularity parameters.

Influences of Friction Stir Welding Parameters on Microstructural and Mechanical Properties of AA5456 (AlMg5) at Different Lap Joint Thicknesses

NASA Astrophysics Data System (ADS)

Pishevar, M. R.; Mohandesi, J. Aghazadeh; Omidvar, H.; Safarkhanian, M. A.

2015-10-01

Friction stir welding is suitable for joining series 5000 alloys because no fusion welding problems arise for the alloys in this process. The present study examined the effects of double-pass welding and tool rotational and travel speeds for the second-pass welding on the mechanical and microstructural properties of friction stir lap welding of AA5456 (AlMg5)-H321 (5 mm thickness) and AA5456 (AlMg5)-O (2.5 mm thickness). The first pass of all specimens was performed at a rotational speed of 650 rpm and a travel speed of 50 mm/min. The second pass was performed at rotational speeds of 250, 450, and 650 rpm and travel speeds of 25, 50, and 75 mm/min. The results showed that the second pass changed the grain sizes in the center of the nugget zone compared with the first pass. It was observed that the size of the hooking defect of the double-pass-welded specimens was higher than that for the single-pass-welded specimen. The size of the hooking defect was found to be a function of the rotational and travel speeds. The optimal joint tensile shear properties were achieved at a rotational speed of 250 rpm and travel a speed of 75 mm/min.

Sandia/Stanford Unified Creep Plasticity Damage Model for ANSYS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pierce, David M.; Vianco, Paul T.; Fossum, Arlo F.

2006-09-03

A unified creep plasticity (UCP) model was developed, based upon the time-dependent and time-independent deformation properties of the 95.5Sn-3.9Ag-0.6Cu (wt.%) soldier that were measured at Sandia. Then, a damage parameter, D, was added to the equation to develop the unified creep plasticity damage (UCPD) model. The parameter, D, was parameterized, using data obtained at Sandia from isothermal fatigue experiments on a double-lap shear test. The softwae was validated against a BGA solder joint exposed to thermal cycling. The UCPD model was put into the ANSYS finite element as a subroutine. So, the softwae is the subroutine for ANSYS 8.1.

Bond strength evaluation in adhesive joints using NDE and DIC methods

NASA Astrophysics Data System (ADS)

Poudel, Anish

Adhesive bonding of graphite epoxy composite laminates to itself or traditional metal alloys in modern aerospace and aircraft structural applications offers an excellent opportunity to use the most efficient and intelligent combination of materials available thus providing an attractive package for efficient structural designs. However, one of the major issues of adhesive bonding is the occasional formation of interfacial defects such as kissing or weak bonds in the bondline interface. Also, there are shortcomings of existing non-destructive evaluation (NDE) methods to non-destructively detect/characterize these interfacial defects and reliably predicting the bond shear strength. As a result, adhesive bonding technology is still not solely implemented in primary structures of an aircraft. Therefore, there is a greater demand for a novel NDE tool that can meet the existing aerospace requirement for adhesive bondline characterization. This research implemented a novel Acoustography ultrasonic imaging and digital image correlation (DIC) technique to detect and characterize interfacial defects in the bondline and determine bond shear strength in adhesively bonded composite-metal joints. Adhesively bonded Carbon Fiber Reinforced Plastic (CFRP) laminate and 2024-T3 Aluminum single lap shear panels subjected to various implanted kissing/weak bond defects were the primary focus of this study. Kissing/weak bonds were prepared by controlled surface contamination in the composite bonding surface and also by improperly mixing the adhesive constituent. SEM analyses were also conducted to understand the surface morphology of substrates and their interaction with the contaminants. Morphological changes were observed in the microscopic scale and the chemical analysis confirmed the stability of the contaminant at or very close to the interface. In addition, it was also demonstrated that contaminants migrated during the curing of the adhesive from CFRP substrate which caused a decrease of bond shear strength in single lap shear test samples. Through-transmission ultrasonics (TTU) Acoustography at 3.8 MHz showed promising results on the detectability of bondline defects in adhesively bonded CFRP-Al lap shear test samples. A correlation between Acoustography ultrasonic attenuation and average bond shear strength in CFRP-Al lap shear panels demonstrated that differential attenuation increased with the reduction of the bond shear strength. Similarly, optical DIC tests were conducted to identify and quantify kissing bond defects in CFRP-Al single lap shear joints. DIC results demonstrated changes in the normal strain (epsilonyy) contour map of the contaminated specimens at relatively lower load levels (15% ~ 30% of failure loads). Kissing bond regions were characterized by negative strains, and these were attributed to high compressive bending strains and the localized disbonding taking placed at the bondline interface as a result of the load application. It was also observed that contaminated samples suffered from more compressive strains (epsilonyy) compared to the baseline sample along the loading direction and they suffered from less compressive strains (epsilonxx) compared to the baseline sample perpendicular to the loading direction. This demonstrated the adverse effect of the kissing bond on the adhesive joint integrity. This was a very significant finding for the reason that hybrid ultrasonic DIC is being developed as a faster, more efficient, and more reliable NDE technique for determining bond quality and predicting bond shear strength in adhesively bonded structures.

Microstructure and Properties of Lap Joint Between Aluminum Alloy and Galvanized Steel by CMT

NASA Astrophysics Data System (ADS)

Niu, Song; Chen, Su; Dong, Honggang; Zhao, Dongsheng; Zhang, Xiaosheng; Guo, Xin; Wang, Guoqiang

2016-05-01

Lap joining of 1-mm-thick Novelist AC 170 PX aluminum alloy to 1.2-mm-thick ST06 Z galvanized steel sheets for automotive applications was conducted by cold metal transfer advanced welding process with ER4043 and ER4047 filler wires. Under the optimized welding parameters with ER4043 filler wire, the tensile shear strength of joint was 189 MPa, reaching 89% of the aluminum alloy base metal. Microstructure and elemental distribution were characterized by optical metalloscope and electron probe microanalysis. The lap joints with ER4043 filler wire had smaller wetting angle and longer bonded line length with better wettability than with ER4047 filler wire during welding with same parameters. The needle-like Al-Fe-Si intermetallic compounds (IMCs) were spalled into the weld and brought negative effect to the tensile strength of joints. With increasing welding current, the needle-like IMCs grew longer and spread further into the weld, which would deteriorate the tensile shear strength.

Delamination properity of 2060 aluminium lithium alloy laminate

NASA Astrophysics Data System (ADS)

Guo, Xiang; Huang, Yi; Tong, Dihua; Ma, Shaojun

2018-03-01

Delamination is an significant property of aluminium lithium alloy laminate for damage tolerance design. Double Crack Lap Shear specimens of "2/1" structure are used for testing delamination properity. Experiments of 2 stress ratio conditions R=0.06, 0.5 are conducted, and 5 stress levels for each stress ratio. Delamination growth data b-N are obtained from 4 crack tips’ locations. The energy release rate, Gd, of the "2/1" structure laminate are calculated. Alderliesten modelis used for describing delamination growth db/dN and energy release rate Gd. Parameters Cd and nd in Alderliesten model are determined for R=0.06 and R=0.5 by linear fitting. An analysis is made by comparing the results of different stress ratio conditons.

Lap Shear and Impact Testing of Ochre and Beeswax in Experimental Middle Stone Age Compound Adhesives.

PubMed

Kozowyk, P R B; Langejans, G H J; Poulis, J A

2016-01-01

The production of compound adhesives using disparate ingredients is seen as some of the best evidence of advanced cognition outside of the use of symbolism. Previous field and laboratory testing of adhesives has shown the complexities involved in creating an effective Middle Stone Age glue using Acacia gum. However, it is currently unclear how efficient different adhesive recipes are, how much specific ingredients influence their performance, and how difficult it may have been for those ingredients to be combined to maximum effect. We conducted a series of laboratory-based lap shear and impact tests, following modern adhesion testing standards, to determine the efficacy of compound adhesives, with particular regard to the ingredient ratios. We tested rosin (colophony) and gum adhesives, containing additives of beeswax and ochre in varying ratios. During both lap shear and impact tests compound rosin adhesives performed better than single component rosin adhesives, and pure acacia gum was the strongest. The large difference in performance between each base adhesive and the significant changes in performance that occur due to relatively small changes in ingredient ratios lend further support to the notion that high levels of skill and knowledge were required to consistently produce the most effective adhesives.

Lap Shear and Impact Testing of Ochre and Beeswax in Experimental Middle Stone Age Compound Adhesives

PubMed Central

2016-01-01

The production of compound adhesives using disparate ingredients is seen as some of the best evidence of advanced cognition outside of the use of symbolism. Previous field and laboratory testing of adhesives has shown the complexities involved in creating an effective Middle Stone Age glue using Acacia gum. However, it is currently unclear how efficient different adhesive recipes are, how much specific ingredients influence their performance, and how difficult it may have been for those ingredients to be combined to maximum effect. We conducted a series of laboratory-based lap shear and impact tests, following modern adhesion testing standards, to determine the efficacy of compound adhesives, with particular regard to the ingredient ratios. We tested rosin (colophony) and gum adhesives, containing additives of beeswax and ochre in varying ratios. During both lap shear and impact tests compound rosin adhesives performed better than single component rosin adhesives, and pure acacia gum was the strongest. The large difference in performance between each base adhesive and the significant changes in performance that occur due to relatively small changes in ingredient ratios lend further support to the notion that high levels of skill and knowledge were required to consistently produce the most effective adhesives. PMID:26983080

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sabau, Adrian S; Warren, Charles David; ERDMAN III, DONALD L

Due to its increased use in the automotive and aerospace industries, joining of Carbon Fiber-reinforced Polymer matrix Composites (CFPC) to metals demands enhanced surface preparation and control of surface morphology prior to joining. In this study, surfaces of both composite and aluminum were prepared for joining using a new laser based technique, in which the laser interference power profile was created by splitting the beam and guiding those beams to the sample surface by overlapping each other with defined angles to each other. Results were presented for the overlap shear testing of single-lap joints made with Al 5182 and CFPCmore » specimens whose surfaces prepared by (a) surface abrasion and solvent cleaning; and (b) laser-interference structured surfaces by rastering with a 4 mm laser beam at approximately 3.5 W power. CFPC specimens of T700S carbon fiber, Prepreg T70 epoxy, 4 or 5 ply thick, 0/90o plaques were used. Adhesive DP810 was used to bond Al and CFPC. The bondline was 0.25mm and the bond length was consistent among all joints produced. First, the effect of the laser speed on the joint performance was evaluated by laser-interference structure Al and CFPC surfaces with a beam angle of 3o and laser beam speeds of 3, 5, and 10 mm/s. For this sensitivity study, 3 joint specimens were used per each joint type. Based on the results for minimum, maximum, and mean values for the shear lap strength and maximum load for all the 9 joint types, two joint types were selected for further evaluations. Six additional joint specimens were prepared for these two joint types in order to obtain better statistics and the shear test data was presented for the range, mean, and standard deviation. The results for the single-lap shear tests obtained for six joint specimens, indicate that the shear lap strength, maximum load, and displacement at maximum load for those joints made with laser-interference structured surfaces were increased by approximately 14.8%, 16%, and 100%, respectively over those measured for the baseline joints.« less

Fatigue properties of dissimilar metal laser welded lap joints

NASA Astrophysics Data System (ADS)

Dinsley, Christopher Paul

This work involves laser welding austenitic and duplex stainless steel to zinc-coated mild steel, more specifically 1.2mm V1437, which is a Volvo Truck Coiporation rephosphorised mild steel. The work investigates both tensile and lap shear properties of similar and dissimilar metal laser welded butt and lap joints, with the majority of the investigation concentrating on the fatigue properties of dissimilar metal laser welded lap joints. The problems encountered when laser welding zinc-coated steel are addressed and overcome with regard to dissimilar metal lap joints with stainless steel. The result being the production of a set of guidelines for laser welding stainless steel to zinc-coated mild steel. The stages of laser welded lap joint fatigue life are defined and the factors affecting dissimilar metal laser welded lap joint fatigue properties are analysed and determined; the findings suggesting that dissimilar metal lap joint fatigue properties are primarily controlled by the local stress at the internal lap face and the early crack growth rate of the material at the internal lap face. The lap joint rotation, in turn, is controlled by sheet thickness, weld width and interfacial gap. Laser welded lap joint fatigue properties are found to be independent of base material properties, allowing dissimilar metal lap joints to be produced without fatigue failure occurring preferentially in the weaker parent material, irrespective of large base material property differences. The effects of Marangoni flow on the compositions of the laser weld beads are experimentally characterised. The results providing definite proof of the stirring mechanism within the weld pool through the use of speeds maps for chromium and nickel. Keywords: Laser welding, dissimilar metal, Zinc-coated mild steel, Austenitic stainless steel, Duplex stainless steel, Fatigue, Lap joint rotation, Automotive.

Bonded joint and method. [for reducing peak shear stress in adhesive bonds

NASA Technical Reports Server (NTRS)

Sainsbury-Carter, J. B. (Inventor)

1974-01-01

An improved joint is described for reducing the peak shear stress in adhesive bonds when adhesives are used to bond two materials which are in a lapped relationship and which differ in value of modulus of elasticity. An insert placed between the adhesive and one of the two materials acts to cushion the discontinuity of material stiffness thereby reducing the peak shear stress in the adhesive bond.

Lap shear strength and healing capability of self-healing adhesive containing epoxy/mercaptan microcapsules

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ghazali, Habibah; Ye, Lin; Zhang, Ming-Qiu

The aim of this work is to develop a self-healing polymeric adhesive formulation with epoxy/mercaptan microcapsules. Epoxy/mercaptan microcapsules were dispersed into a commercialize two-part epoxy adhesive for developing self-healing epoxy adhesive. The influence of different content of microcapsules on the shear strength and healing capability of epoxy adhesive were investigated using single-lap-joints with average thickness of adhesive layer of about 180 µm. This self-healing adhesive was used in bonding of 5000 series aluminum alloys adherents after mechanical and alkaline cleaning surface treatment. The adhesion strength was measured and presented as function of microcapsules loading. The results indicated that the virgin lapmore » shear strength was increased by about 26% with addition of 3 wt% of self-healing microcapsules. 12% to 28% recovery of the shear strength is achieved after self-healing depending on the microcapsules content. Scanning electron microscopy was used to study fracture surface of the joints. The self-healing adhesives exhibit recovery of both cohesion and adhesion properties with room temperature healing.« less

15 CFR 700.31 - Metalworking machines.

Code of Federal Regulations, 2011 CFR

2011-01-01

... machines covered by this section include: Bending and forming machines Boring machines Broaching machines... Planers and shapers Polishing, lapping, boring, and finishing machines Punching and shearing machines...

15 CFR 700.31 - Metalworking machines.

Code of Federal Regulations, 2012 CFR

2012-01-01

... machines covered by this section include: Bending and forming machines Boring machines Broaching machines... Planers and shapers Polishing, lapping, boring, and finishing machines Punching and shearing machines...

15 CFR 700.31 - Metalworking machines.

Code of Federal Regulations, 2013 CFR

2013-01-01

... machines covered by this section include: Bending and forming machines Boring machines Broaching machines... Planers and shapers Polishing, lapping, boring, and finishing machines Punching and shearing machines...

15 CFR 700.31 - Metalworking machines.

Code of Federal Regulations, 2014 CFR

2014-01-01

... machines covered by this section include: Bending and forming machines Boring machines Broaching machines... Planers and shapers Polishing, lapping, boring, and finishing machines Punching and shearing machines...

15 CFR 700.31 - Metalworking machines.

Code of Federal Regulations, 2010 CFR

2010-01-01

... machines covered by this section include: Bending and forming machines Boring machines Broaching machines... Planers and shapers Polishing, lapping, boring, and finishing machines Punching and shearing machines...

Conformational co-dependence between Plasmodium berghei LCCL proteins promotes complex formation and stability.

PubMed

Saeed, Sadia; Tremp, Annie Z; Dessens, Johannes T

2012-10-01

Malaria parasites express a conserved family of LCCL-lectin adhesive-like domain proteins (LAPs) that have essential functions in sporozoite transmission. In Plasmodium falciparum all six family members are expressed in gametocytes and form a multi-protein complex. Intriguingly, knockout of P. falciparum LCCL proteins adversely affects expression of other family members at protein, but not at mRNA level, a phenomenon termed co-dependent expression. Here, we investigate this in Plasmodium berghei by crossing a PbLAP1 null mutant parasite with a parasite line expressing GFP-tagged PbLAP3 that displays strong fluorescence in gametocytes. Selected and validated double mutants show normal synthesis and subcellular localization of PbLAP3::GFP. However, GFP-based fluorescence is dramatically reduced without PbLAP1 present, indicating that PbLAP1 and PbLAP3 interact. Moreover, absence of PbLAP1 markedly reduces the half-life of PbLAP3, consistent with a scenario of misfolding. These findings unveil a potential mechanism of conformational interdependence that facilitates assembly and stability of the functional LCCL protein complex. Copyright © 2012 Elsevier B.V. All rights reserved.

Microstructure and Mechanical Properties of an Ultrasonic Spot Welded Aluminum Alloy: The Effect of Welding Energy

PubMed Central

Peng, He; Chen, Daolun; Jiang, Xianquan

2017-01-01

The aim of this study is to evaluate the microstructures, tensile lap shear strength, and fatigue resistance of 6022-T43 aluminum alloy joints welded via a solid-state welding technique–ultrasonic spot welding (USW)–at different energy levels. An ultra-fine necklace-like equiaxed grain structure is observed along the weld line due to the occurrence of dynamic crystallization, with smaller grain sizes at lower levels of welding energy. The tensile lap shear strength, failure energy, and critical stress intensity of the welded joints first increase, reach their maximum values, and then decrease with increasing welding energy. The tensile lap shear failure mode changes from interfacial fracture at lower energy levels, to nugget pull-out at intermediate optimal energy levels, and to transverse through-thickness (TTT) crack growth at higher energy levels. The fatigue life is longer for the joints welded at an energy of 1400 J than 2000 J at higher cyclic loading levels. The fatigue failure mode changes from nugget pull-out to TTT crack growth with decreasing cyclic loading for the joints welded at 1400 J, while TTT crack growth mode remains at all cyclic loading levels for the joints welded at 2000 J. Fatigue crack basically initiates from the nugget edge, and propagates with “river-flow” patterns and characteristic fatigue striations. PMID:28772809

Microstructure and Mechanical Properties of an Ultrasonic Spot Welded Aluminum Alloy: The Effect of Welding Energy.

PubMed

Peng, He; Chen, Daolun; Jiang, Xianquan

2017-04-25

The aim of this study is to evaluate the microstructures, tensile lap shear strength, and fatigue resistance of 6022-T43 aluminum alloy joints welded via a solid-state welding technique-ultrasonic spot welding (USW)-at different energy levels. An ultra-fine necklace-like equiaxed grain structure is observed along the weld line due to the occurrence of dynamic crystallization, with smaller grain sizes at lower levels of welding energy. The tensile lap shear strength, failure energy, and critical stress intensity of the welded joints first increase, reach their maximum values, and then decrease with increasing welding energy. The tensile lap shear failure mode changes from interfacial fracture at lower energy levels, to nugget pull-out at intermediate optimal energy levels, and to transverse through-thickness (TTT) crack growth at higher energy levels. The fatigue life is longer for the joints welded at an energy of 1400 J than 2000 J at higher cyclic loading levels. The fatigue failure mode changes from nugget pull-out to TTT crack growth with decreasing cyclic loading for the joints welded at 1400 J, while TTT crack growth mode remains at all cyclic loading levels for the joints welded at 2000 J. Fatigue crack basically initiates from the nugget edge, and propagates with "river-flow" patterns and characteristic fatigue striations.

Modeling and Measurement of Sustained Loading and Temperature-Dependent Deformation of Carbon Fiber-Reinforced Polymer Bonded to Concrete.

PubMed

Jeong, Yoseok; Lee, Jaeha; Kim, WooSeok

2015-01-29

This paper aims at presenting the effects of short-term sustained load and temperature on time-dependent deformation of carbon fiber-reinforced polymer (CFRP) bonded to concrete and pull-off strength at room temperature after the sustained loading period. The approach involves experimental and numerical analysis. Single-lap shear specimens were used to evaluate temperature and short-term sustained loading effects on time-dependent behavior under sustained loading and debonding behavior under pull-off loading after a sustained loading period. The numerical model was parameterized with experiments on the concrete, FRP, and epoxy. Good correlation was seen between the numerical results and single-lap shear experiments. Sensitivity studies shed light on the influence of temperature, epoxy modulus, and epoxy thickness on the redistribution of interfacial shear stress during sustained loading. This investigation confirms the hypothesis that interfacial stress redistribution can occur due to sustained load and elevated temperature and its effect can be significant.

Modeling and Measurement of Sustained Loading and Temperature-Dependent Deformation of Carbon Fiber-Reinforced Polymer Bonded to Concrete

PubMed Central

Jeong, Yoseok; Lee, Jaeha; Kim, WooSeok

2015-01-01

This paper aims at presenting the effects of short-term sustained load and temperature on time-dependent deformation of carbon fiber-reinforced polymer (CFRP) bonded to concrete and pull-off strength at room temperature after the sustained loading period. The approach involves experimental and numerical analysis. Single-lap shear specimens were used to evaluate temperature and short-term sustained loading effects on time-dependent behavior under sustained loading and debonding behavior under pull-off loading after a sustained loading period. The numerical model was parameterized with experiments on the concrete, FRP, and epoxy. Good correlation was seen between the numerical results and single-lap shear experiments. Sensitivity studies shed light on the influence of temperature, epoxy modulus, and epoxy thickness on the redistribution of interfacial shear stress during sustained loading. This investigation confirms the hypothesis that interfacial stress redistribution can occur due to sustained load and elevated temperature and its effect can be significant. PMID:28787948

Cyclic debonding of adhesively bonded composites

NASA Technical Reports Server (NTRS)

Mall, S.; Johnson, W. S.; Everett, R. A., Jr.

1982-01-01

The fatigue behavior of a simple composite to composite bonded joint was analyzed. The cracked lap shear specimen subjected to constant amplitude cyclic loading was studied. Two specimen geometries were tested for each bonded system: (1) a strap adherend of 16 plies bonded to a lap adherend of 8 plies; and (2) a strap adherend of 8 plies bonded to a lap adherend of 16 plies. In all specimens the fatigue failure was in the form of cyclic debonding with some 0 deg fiber pull off from the strap adherend. The debond always grew in the region of adhesive that had the highest mode (peel) loading and that region was close to the adhesive strap interface.

In Situ Mechanical Property Measurements of Amorphous Carbon-Boron Nitride Nanotube Nanostructures

NASA Technical Reports Server (NTRS)

Kim, Jae-Woo; Lin, Yi; Nunez, Jennifer Carpena; Siochi, Emilie J.; Wise, Kristopher E.; Connell, John W.; Smith, Michael W.

2011-01-01

To understand the mechanical properties of amorphous carbon (a-C)/boron nitride nanotube (BNNT) nanostructures, in situ mechanical tests are conducted inside a transmission electron microscope equipped with an integrated atomic force microscope system. The nanotube structure is modified with amorphous carbon deposited by controlled electron beam irradiation. We demonstrate multiple in situ tensile, compressive, and lap shear tests with a-C/BNNT hybrid nanostructures. The tensile strength of the a-C/BNNT hybrid nanostructure is 5.29 GPa with about 90 vol% of a-C. The tensile strength and strain of the end-to-end joint structure with a-C welding is 0.8 GPa and 5.2% whereas the lap shear strength of the side-by-side joint structure with a-C is 0.25 GPa.

Design, fabrication and test of graphite/polyimide composite joints and attachments for advanced aerospace vehicles

NASA Technical Reports Server (NTRS)

Skoumal, D. E.

1980-01-01

Bonded and bolted designs are presented for each of four major attachment types. Prepreg processing problems are discussed and quality control data are given for lots 2W4604, 2W4632 and 2W4643. Preliminary design allowables test results for tension tests and compression tests of laminates are included. The final small specimen test matrix is defined and the configuration of symmetric step-lap joint specimens are shown. Finite element modeling studies of a double lap joint were performed to evaluate the number of elements required through the adhesive thickness to assess effects of various joint parameters on stress distributions. Results of finite element analyses assessing the effect of an adhesive fillet on the stress distribution in a double lap joint are examined.

Characteristics of solder joints under fatigue loads using piezomechanical actuation

NASA Astrophysics Data System (ADS)

Shim, Dong-Jin; Spearing, S. Mark

2003-07-01

Crack initiation and growth characteristics of solder joints under fatigue loads are investigated using piezomechanical actuation. Cracks in solder joints, which can cause failure in microelectronics components, are induced via piezoelectricity in piezo-ceramic bonded joints. Lead-zirconate-titanate ceramic plates and eutectic Sn-Pb solder bonded in a double-lap shear configuration are used in the investigation. Electric field across each piezo-ceramic plate is applied such that shear stresses/strains are induced in the solder joints. The experiments show that cracks initiate in the solder joints around defects such as voids and grow in length until they coalesce with other cracks from adjacent voids. These observations are compared with the similar thermal cycling tests from the literature to show feasibility and validity of the current method in investigating the fatigue characteristics of solder joints. In some specimens, cracks in the piezo-ceramic plates are observed, and failure in the specimens generally occurred due to piezo-ceramic plate fracture. The issues encountered in implementing this methodology such as low actuation and high processing temperatures are further discussed.

Microstructure and mechanical properties of friction stir lap welded Mg/Al joint assisted by stationary shoulder

NASA Astrophysics Data System (ADS)

Ji, Shude; Li, Zhengwei

2017-11-01

Using magnesium alloy as upper sheet, 3 mm-thick AZ31 magnesium alloy and 6061 aluminum alloy were joined using friction stir lap welding assisted by stationary shoulder. The effects of tool rotating speed on cross-sections, microstructure and mechanical properties of Mg/Al lap joints were mainly discussed. Results showed that stationary shoulder contributed to joint formation, by which stir zones (SZ) were characterized by big onion rings after welding. Because of the big forging force exerted by stationary shoulder, the upper region of hook was well bonded. SZ showed much higher hardness because of intermetallic compounds (IMCs). The bonding conditions at the base material (BM)/SZ interface at advancing side and the hook region played important roles on joint lap shear properties. The X-ray diffraction pattern analysis revealed that the main IMCs were Al3Mg2 and Al12Mg17.

Evaluation of a thermoplastic polyimide (422) for bonding GR/PI composite

NASA Technical Reports Server (NTRS)

Progar, Donald J.

1988-01-01

A hot-melt processable copolyimide previously studied and characterized as an adhesive for bonding Ti-6Al-4V was used to bond Celion 6000/LARC-160 composite. Comparisons are made for the two adherend systems. A bonding cycle was determined for the composite bonding and lap shear specimens were prepared which were thermally exposed in a forced-air oven for up to 5000 h at 204 C. The lap shear strengths (LSSs) were determined at RT, 177, and 204 C. After thermal exposure at RT, 177, and 204 C the LSS decreased significantly; however, a slight increase was noted for the 204 C tests. Initially the LSS values are higher for the bonded Ti-6Al-4V than for the bonded composite, however, the LSS decreases dramatically between 5000 and 10,000 h of 204 C thermal exposure. Longer periods of thermal exposure up to 20,000 h results in further decreases in the LSSs. Although the bonded composite retained useful strengths for exposures up to 5000 h, based on the poor results of the bonded Ti-6Al-4V beyond 5000 h, the 422 adhesive bonded composites would most likely also produce poor strengths beyond 5000 h exposure. Adhesive bonded composite lap shear specimens exposed to boiling water for 72 h exhibited greatly reduced strengths at all test temperatures. The percent retained after water boil for each test temperature was essentially the same for both systems.

Evaluation of a thermoplastic polyimide (422) for bonding GR/PI composite

NASA Technical Reports Server (NTRS)

Progar, Donald J.

1988-01-01

A hot-melt processable copolyimide previously studied and characterized as an adhesive for bonding Ti-6Al-4V was used to bond Celion 6000/LARC-160 composite. Comparisons are made for the two adherend systems. A bonding cycle was determined for the composite bonding and lap shear specimens were prepared which were thermally exposed in a forced-air oven for up to 5000 h at 204 C. The lap shear strengths (LSSs) were determined at RT, 177, and 204 C. After thermal exposure at RT, 177, and 204 C the LSS decreased significantly; however, a slight increase was noted for the 204 C tests. Initially the LSS values are higher for the bonded Ti-6Al-4V than for the bonded composite, however, the LSS decreases dramatically between 5000 and 10,000 h of 204 C thermal exposure. Longer periods of thermal exposure up to 20,000 h results in further decreases in the LSSs. Although the bonded composite retained useful strengths for exposures up to 5000 h, based on the por results of the bonded Ti-6Al-4V beyond 5000 h, the 422 adhesive bonded composites would most likely also produce poor strengths beyond 5000 h exposure. Adhesive bonded composite lap shear specimens exposed to boiling water for 72 h exhibited greatly reduced strengths at all test temperatures. The percent retained after water boil for each test temperature was essentially the same for both systems.

Bolted joints in graphite-epoxy composites

NASA Technical Reports Server (NTRS)

Hart-Smith, L. J.

1976-01-01

All-graphite/epoxy laminates and hybrid graphite-glass/epoxy laminates were tested. The tests encompassed a range of geometries for each laminate pattern to cover the three basic failure modes - net section tension failure through the bolt hole, bearing and shearout. Static tensile and compressive loads were applied. A constant bolt diameter of 6.35 mm (0.25 in.) was used in the tests. The interaction of stress concentrations associated with multi-row bolted joints was investigated by testing single- and double-row bolted joints and open-hole specimens in tension. For tension loading, linear interaction was found to exist between the bearing stress reacted at a given bolt hole and the remaining tension stress running by that hole to be reacted elsewhere. The interaction under compressive loading was found to be non-linear. Comparative tests were run using single-lap bolted joints and double-lap joints with pin connection. Both of these joint types exhibited lower strengths than were demonstrated by the corresponding double-lap joints. The analysis methods developed here for single bolt joints are shown to be capable of predicting the behavior of multi-row joints.

75 FR 68664 - Federal Motor Vehicle Safety Standards; Child Restraint Systems; Booster Seat Effectiveness...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-08

... early graduation from booster seats to lap and shoulder belts. Estimates are computed by double-pair... 3-4-year-olds there is evidence of increased risk of injury when restrained in booster seats rather.... Among 4-8-year-olds there is strong evidence of increased risk of injury when restrained by lap and...

Revisiting the generalized scaling law for adhesion: role of compliance and extension to progressive failure.

PubMed

Mojdehi, Ahmad R; Holmes, Douglas P; Dillard, David A

2017-10-25

A generalized scaling law, based on the classical fracture mechanics approach, is developed to predict the bond strength of adhesive systems. The proposed scaling relationship depends on the rate of change of debond area with compliance, rather than the ratio of area to compliance. This distinction can have a profound impact on the expected bond strength of systems, particularly when the failure mechanism changes or the compliance of the load train increases. Based on the classical fracture mechanics approach for rate-independent materials, the load train compliance should not affect the force capacity of the adhesive system, whereas when the area to compliance ratio is used as the scaling parameter, it directly influences the bond strength, making it necessary to distinguish compliance contributions. To verify the scaling relationship, single lap shear tests were performed for a given pressure sensitive adhesive (PSA) tape specimens with different bond areas, number of backing layers, and load train compliance. The shear lag model was used to derive closed-form relationships for the system compliance and its derivative with respect to the debond area. Digital image correlation (DIC) is implemented to verify the non-uniform shear stress distribution obtained from the shear lag model in a lap shear geometry. The results obtained from this approach could lead to a better understanding of the relationship between bond strength and the geometry and mechanical properties of adhesive systems.

Interfacial and Mechanical Behavior of AA5456 Filling Friction-Stir-Welded Lap Joints Using Similar and Dissimilar Pins

NASA Astrophysics Data System (ADS)

Behmand, Saleh Alaei; Mirsalehi, Seyyed Ehsan; Omidvar, Hamid; Safarkhanian, Mohammad Ali

2016-10-01

In this article, filling friction stir welding (FFSW) of the remaining exit holes of AA5456 alloy friction-stir-welded lap joints was studied. For this purpose, the influences of different rotating speeds, holding times, and pin materials, AA5456 and AA2024, on the metallurgical structure and joint strength were investigated. The observations showed that defect-free lap joints are successfully obtainable by this method using similar and dissimilar consumable pins. The results indicated that the higher rotating speed and holding time adversely affect the weld performance. The best result was achieved for 30 seconds holding time, 500 rpm rotating speed, and AA2024 consumable pin. In this condition, a lap shear strength of 10 pct higher than that of the nonfilled joint, equivalent to about 94 pct of the original defect-free FSW joint, was obtained, whereas the GTAW filled joint showed only approximately 87 pct of the continuous FSW joint strength.

Evaluation of adhesive materials used on the Long Duration Exposure Facility

NASA Technical Reports Server (NTRS)

Dursch, H. W.; Keough, B. K.; Pippin, H. G.

1995-01-01

The adhesive and adhesive-like materials flown on LDEF included epoxies and silicones (including lap shear specimens), conformal coatings, potting compounds, and several tapes and transfer films. With the exception of the lap shear specimens, these materials were used in the fabrication and assembly of the experiments such as bonding thermal control surfaces to other hardware and holding individual specimens in place, similar to applications on other spacecraft. Typically, the adhesives were not exposed to solar radiation or atomic oxygen. Only one adhesive system was used in a structural application. This report documents all results of the Materials and Systems SIG investigation into the effect of long term low Earth orbit (LEO) exposure of these materials. Results of this investigation show that if the material was shielded from exposure to LDEF's external environment, the 69 month exposure to LEO had, in most cases, minimal effect on the material.

Fracture mechanics approach to estimate fatigue lives of welded lap-shear specimens

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lam, Poh -Sang; Pan, Jwo

A full range of stress intensity factor solutions for a kinked crack with finite length is developed as a function of weld width and the sheet thickness. When used with the main crack solutions (global stress intensity factors) in terms of the applied load and the specimen geometric parameters, the fatigue lived of the kinked crack can be estimated for the laser-welded lap-shear specimens. The predicted curve for the load range-fatigue life passes through the cluster of experimental data and is in good agreement. A classical solution associated with an infinitesimal kink is also employed. Furthermore, its life prediction tendsmore » to overestimate the actual fatigue life. In addition, the traditional fatigue life estimation based on structural stress is performed for completeness. As a result, this non-fracture mechanics approach only agrees well with the experimental data under high cyclic load conditions.« less

An investigation of adhesive/adherend and fiber/matrix interactions. Part B: SEM/ESCA analysis of fracture surfaces

NASA Technical Reports Server (NTRS)

Beck, B.; Widyani, E.; Wightman, J. P.

1983-01-01

Adhesion was studied with emphasis on the characterization of surface oxide layers, the analysis of fracture surfaces, and the interaction of matrices and fibers. A number of surface features of the fractured lap shear samples were noted in the SEM photomicrographs including the beta phase alloy of the Ti 6-4 adherend, the imprint of the adherend on the adhesive failure surface, increased void density for high temperature samples, and the alumina filler particles. Interfacial failure of some of the fractured lap shear samples is invariably characterized by the appearance of an ESCA oxygen photopeak at 530.3 eV assigned to the surface oxide layer of Ti 6-4 adherend. The effect of grit blasting on carbon fiber composites is evident in the SEM analysis. A high surface fluorine concentration on the composite surface is reduced some ten fold by grit blasting.

Fracture mechanics approach to estimate fatigue lives of welded lap-shear specimens

DOE PAGES

Lam, Poh -Sang; Pan, Jwo

2015-06-29

A full range of stress intensity factor solutions for a kinked crack with finite length is developed as a function of weld width and the sheet thickness. When used with the main crack solutions (global stress intensity factors) in terms of the applied load and the specimen geometric parameters, the fatigue lived of the kinked crack can be estimated for the laser-welded lap-shear specimens. The predicted curve for the load range-fatigue life passes through the cluster of experimental data and is in good agreement. A classical solution associated with an infinitesimal kink is also employed. Furthermore, its life prediction tendsmore » to overestimate the actual fatigue life. In addition, the traditional fatigue life estimation based on structural stress is performed for completeness. As a result, this non-fracture mechanics approach only agrees well with the experimental data under high cyclic load conditions.« less

Effects of different surface treatments on the bond strength of acrylic denture teeth to polymethylmethacrylate denture base material.

PubMed

Akin, Hakan; Kirmali, Omer; Tugut, Faik; Coskun, Mehmet Emre

2014-09-01

The purpose of this study was to investigate the effects of various surface pretreatments in the ridge lap area of acrylic resin denture teeth on the shear bond strength to heat-polymerized polymethylmethacrylate (PMMA) denture base resin. Tooth debonding of the denture is a major problem for patients with removable prostheses. A total of 84 central incisor denture teeth were used in this study. Seven test groups with 12 specimens for each group were prepared as follows: untreated (control, group C), ground, with a tungsten carbide bur (group H), airborne-particle abrasion (group AA), primed with methyl methacrylate (group M), treated with izobutyl methacrylate (group iBMA), Eclipse Bonding Agent applied (group E), and Er:YAG laser irradiated (group L). Test specimens were produced according to the manufacturers' instructions and mounted to a universal testing machine for shear testing with a crosshead speed of 1 mm/min. Data were evaluated by one way variance analysis (ANOVA) and Tukey's test (α=0.05). Similar bond strength values were found between groups L and M, and these were the highest shear bond strengths among the groups. The lowest one was observed in group E. All surface treatments, except group E, exhibited significant difference when compared with group C (p<0.05). Lasing of the ridge lap area to enhance the bond strength of acrylic resin denture teeth to PMMA denture base resin might be an alternative to wetting with MMA monomer. To overcome tooth debonding, surface treatment of the ridge lap area should be performed as part of denture fabrication.

Ultrasonic Welding of Thermoplastic Composite Coupons for Mechanical Characterization of Welded Joints through Single Lap Shear Testing.

PubMed

Villegas, Irene F; Palardy, Genevieve

2016-02-11

This paper presents a novel straightforward method for ultrasonic welding of thermoplastic-composite coupons in optimum processing conditions. The ultrasonic welding process described in this paper is based on three main pillars. Firstly, flat energy directors are used for preferential heat generation at the joining interface during the welding process. A flat energy director is a neat thermoplastic resin film that is placed between the parts to be joined prior to the welding process and heats up preferentially owing to its lower compressive stiffness relative to the composite substrates. Consequently, flat energy directors provide a simple solution that does not require molding of resin protrusions on the surfaces of the composite substrates, as opposed to ultrasonic welding of unreinforced plastics. Secondly, the process data provided by the ultrasonic welder is used to rapidly define the optimum welding parameters for any thermoplastic composite material combination. Thirdly, displacement control is used in the welding process to ensure consistent quality of the welded joints. According to this method, thermoplastic-composite flat coupons are individually welded in a single lap configuration. Mechanical testing of the welded coupons allows determining the apparent lap shear strength of the joints, which is one of the properties most commonly used to quantify the strength of thermoplastic composite welded joints.

Ultrasonic Welding of Thermoplastic Composite Coupons for Mechanical Characterization of Welded Joints through Single Lap Shear Testing

PubMed Central

Villegas, Irene F.; Palardy, Genevieve

2016-01-01

This paper presents a novel straightforward method for ultrasonic welding of thermoplastic-composite coupons in optimum processing conditions. The ultrasonic welding process described in this paper is based on three main pillars. Firstly, flat energy directors are used for preferential heat generation at the joining interface during the welding process. A flat energy director is a neat thermoplastic resin film that is placed between the parts to be joined prior to the welding process and heats up preferentially owing to its lower compressive stiffness relative to the composite substrates. Consequently, flat energy directors provide a simple solution that does not require molding of resin protrusions on the surfaces of the composite substrates, as opposed to ultrasonic welding of unreinforced plastics. Secondly, the process data provided by the ultrasonic welder is used to rapidly define the optimum welding parameters for any thermoplastic composite material combination. Thirdly, displacement control is used in the welding process to ensure consistent quality of the welded joints. According to this method, thermoplastic-composite flat coupons are individually welded in a single lap configuration. Mechanical testing of the welded coupons allows determining the apparent lap shear strength of the joints, which is one of the properties most commonly used to quantify the strength of thermoplastic composite welded joints. PMID:26890931

Failure of the Porcine Ascending Aorta: Multidirectional Experiments and a Unifying Microstructural Model

PubMed Central

Witzenburg, Colleen M.; Dhume, Rohit Y.; Shah, Sachin B.; Korenczuk, Christopher E.; Wagner, Hallie P.; Alford, Patrick W.; Barocas, Victor H.

2017-01-01

The ascending thoracic aorta is poorly understood mechanically, especially its risk of dissection. To make better predictions of dissection risk, more information about the multidimensional failure behavior of the tissue is needed, and this information must be incorporated into an appropriate theoretical/computational model. Toward the creation of such a model, uniaxial, equibiaxial, peel, and shear lap tests were performed on healthy porcine ascending aorta samples. Uniaxial and equibiaxial tests showed anisotropy with greater stiffness and strength in the circumferential direction. Shear lap tests showed catastrophic failure at shear stresses (150–200 kPa) much lower than uniaxial tests (750–2500 kPa), consistent with the low peel tension (∼60 mN/mm). A novel multiscale computational model, including both prefailure and failure mechanics of the aorta, was developed. The microstructural part of the model included contributions from a collagen-reinforced elastin sheet and interlamellar connections representing fibrillin and smooth muscle. Components were represented as nonlinear fibers that failed at a critical stretch. Multiscale simulations of the different experiments were performed, and the model, appropriately specified, agreed well with all experimental data, representing a uniquely complete structure-based description of aorta mechanics. In addition, our experiments and model demonstrate the very low strength of the aorta in radial shear, suggesting an important possible mechanism for aortic dissection. PMID:27893044

Bolted Double-Lap Composite Joints Under Mechanical and Thermal Loading

NASA Technical Reports Server (NTRS)

Kradinov, V.; Barut, A.; Madenci, E.; Walker, Sandra P. (Technical Monitor)

2000-01-01

This study concerns the determination of the contact stresses and contact region around bolt holes and the bolt load distribution in single- and double-lap joints of composite laminates with arbitrarily located bolts under general mechanical loading conditions and uniform temperature change. The unknown contact stress distribution and contact region between the bolt and laminates and the interaction among the bolts require the bolt load distribution, as well as the contact stresses, to be as part of the solution. The present method is based on the complex potential theory and the variational formulation in order to account for bolt stiffness, bolt-hole clearance, and finite geometry of the composite laminates.

Novel on-demand bioadhesion to soft tissue in wet environments.

PubMed

Mogal, Vishal; Papper, Vladislav; Chaurasia, Alok; Feng, Gao; Marks, Robert; Steele, Terry

2014-04-01

Current methods of tissue fixation rely on mechanical-related technologies developed from the clothing and carpentry industries. Herein, a novel bioadhesive method that allows tuneable adhesion and is also applicable to biodegradable polyester substrates is described. Diazirine is the key functional group that allows strong soft tissue crosslinking and on-demand adhesion based on a free radical mechanism. Plasma post-irradiation grafting makes it possible to graft diazirine onto PLGA substrates. When the diazirine-PLGA films, placed on wetted ex vivo swine aortas, are activated with low intensity UV light, lap shear strength of up to 450 ± 50 mN cm(-2) is observed, which is one order of magnitude higher than hydrogel bioadhesives placed on similar soft tissues. The diazirine-modified PLGA thin films could be added on top of previously developed technologies for minimally invasive surgeries. The present work is focused on the chemistry, grafting, and lap shear strength of the alkyl diazirine-modified PLGA bioadhesive films. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Stress analysis of the cracked-lap-shear specimen - An ASTM round-robin

NASA Technical Reports Server (NTRS)

Johnson, W. S.

1987-01-01

This ASTM Round Robin was conducted to evaluate the state of the art in stress analysis of adhesively bonded joint specimens. Specifically, the participants were asked to calculate the strain-energy-release rate for two different geometry cracked lap shear (CLS) specimens at four different debond lengths. The various analytical techniques consisted of 2- and 3-dimensional finite element analysis, beam theory, plate theory, and a combination of beam theory and finite element analysis. The results were examined in terms of the total strain-energy-release rate and the mode I to mode II ratio as a function of debond length for each specimen geometry. These results basically clustered into two groups: geometric linear or geometric nonlinear analysis. The geometric nonlinear analysis is required to properly analyze the CLS specimens. The 3-D finite element analysis gave indications of edge closure plus some mode III loading. Each participant described his analytical technique and results. Nine laboratories participated.

Stress analysis of the cracked lap shear specimens: An ASTM round robin

NASA Technical Reports Server (NTRS)

Johnson, W. S.

1986-01-01

This ASTM Round Robin was conducted to evaluate the state of the art in stress analysis of adhesively bonded joint specimens. Specifically, the participants were asked to calculate the strain-energy-release rate for two different geometry cracked lap shear (CLS) specimens at four different debond lengths. The various analytical techniques consisted of 2- and 3-dimensional finite element analysis, beam theory, plate theory, and a combination of beam theory and finite element analysis. The results were examined in terms of the total strain-energy-release rate and the mode I to mode II ratio as a function of debond length for each specimen geometry. These results basically clustered into two groups: geometric linear or geometric nonlinear analysis. The geometric nonlinear analysis is required to properly analyze the CLS specimens. The 3-D finite element analysis gave indications of edge closure plus some mode III loading. Each participant described his analytical technique and results. Nine laboratories participated.

Evaluation of adhesive materials used on the Long Duration Exposure Facility. Report, October 1989-January 1995

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dursch, H.W.; Keough, B.K.; Pippin, H.G.

1995-03-01

The adhesive and adhesive-like materials flown on LDEF included epoxies and silicones (including lap shear specimens), conformal coatings, potting compounds, and several tapes and transfer films. With the exception of the lap shear specimens, these materials were used in the fabrication and assembly of the experiments such as bonding thermal control surfaces to other hardware and holding individual specimens in place, similar to applications on other spacecraft. Typically, the adhesives were not exposed to solar radiation or atomic oxygen. Only one adhesive system was used in a structural application. This report documents all results of the Materials and Systems SIGmore » investigation into the effect of long term low Earth orbit (LEO) exposure of these materials. Results of this investigation show that if the material was shielded from exposure to LDEF`s external environment, the 69 month exposure to LEO had, in most cases, minimal effect on the material.« less

Microstructure and Fatigue Properties of Ultrasonic Spot Welded Joints of Aluminum 5754 Alloy

NASA Astrophysics Data System (ADS)

Mirza, F. A.; Macwan, A.; Bhole, S. D.; Chen, D. L.

2016-05-01

The purpose of this investigation was to evaluate the microstructural change, lap shear tensile load, and fatigue resistance of ultrasonic spot welded joints of aluminum 5754 alloy for automotive applications. A unique "necklace"-type structure with very fine equiaxed grains was observed to form along the weld line due to the mechanical interlocking coupled with the occurrence of dynamic recrystallization. The maximum lap shear tensile strength of 85 MPa and the fatigue limit of about 0.5 kN (at 1 × 107 cycles) were achieved. The tensile fracture occurred at the Al/Al interface in the case of lower energy inputs, and at the edge of nugget zone in the case of higher energy inputs. The maximum cyclic stress for the transition of fatigue fracture mode from the transverse through-thickness crack growth to the interfacial failure increased with increasing energy input. Fatigue crack propagation was mainly characterized by the formation of fatigue striations, which usually appeared perpendicular to the fatigue crack propagation.

Nonlinear Analysis of Bonded Composite Single-LAP Joints

NASA Technical Reports Server (NTRS)

Oterkus, E.; Barut, A.; Madenci, E.; Smeltzer, S. S.; Ambur, D. R.

2004-01-01

This study presents a semi-analytical solution method to analyze the geometrically nonlinear response of bonded composite single-lap joints with tapered adherend edges under uniaxial tension. The solution method provides the transverse shear and normal stresses in the adhesive and in-plane stress resultants and bending moments in the adherends. The method utilizes the principle of virtual work in conjunction with von Karman s nonlinear plate theory to model the adherends and the shear lag model to represent the kinematics of the thin adhesive layer between the adherends. Furthermore, the method accounts for the bilinear elastic material behavior of the adhesive while maintaining a linear stress-strain relationship in the adherends. In order to account for the stiffness changes due to thickness variation of the adherends along the tapered edges, their in-plane and bending stiffness matrices are varied as a function of thickness along the tapered region. The combination of these complexities results in a system of nonlinear governing equilibrium equations. This approach represents a computationally efficient alternative to finite element method. Comparisons are made with corresponding results obtained from finite-element analysis. The results confirm the validity of the solution method. The numerical results present the effects of taper angle, adherend overlap length, and the bilinear adhesive material on the stress fields in the adherends, as well as the adhesive, of a single-lap joint

Time-temperature effect in adhesively bonded joints

NASA Technical Reports Server (NTRS)

Delale, F.; Erdogan, F.

1981-01-01

The viscoelastic analysis of an adhesively bonded lap joint was reconsidered. The adherends are approximated by essentially Reissner plates and the adhesive is linearly viscoelastic. The hereditary integrals are used to model the adhesive. A linear integral differential equations system for the shear and the tensile stress in the adhesive is applied. The equations have constant coefficients and are solved by using Laplace transforms. It is shown that if the temperature variation in time can be approximated by a piecewise constant function, then the method of Laplace transforms can be used to solve the problem. A numerical example is given for a single lap joint under various loading conditions.

Effects of vertically aligned carbon nanotubes on shear performance of laminated nanocomposite bonded joints.

PubMed

Askari, Davood; Ghasemi-Nejhad, Mehrdad N

2012-08-01

The main objective is to improve the most commonly addressed weakness of the laminated composites (i.e. delamination due to poor interlaminar strength) using carbon nanotubes (CNTs) as reinforcement between the laminae and in the transverse direction. In this work, a chemical vapor deposition technique has been used to grow dense vertically aligned arrays of CNTs over the surface of chemically treated two-dimensionally woven cloth and fiber tows. The nanoforest-like fabrics can be used to fabricate three-dimensionally reinforced laminated nanocomposites. The presence of CNTs aligned normal to the layers and in-between the layers of laminated composites is expected to considerably enhance the properties of the laminates. To demonstrate the effectiveness of our approach, composite single lap-joint specimens were fabricated for interlaminar shear strength testing. It was observed that the single lap-joints with through-the-thickness CNT reinforcement can carry considerably higher shear stresses and strains. Close examination of the test specimens showed that the failure of samples with CNT nanoforests was completely cohesive, while the samples without CNT reinforcement failed adhesively. This concludes that the adhesion of adjacent carbon fabric layers can be considerably improved owing to the presence of vertically aligned arrays of CNT nanoforests.

Effects of vertically aligned carbon nanotubes on shear performance of laminated nanocomposite bonded joints

PubMed Central

Askari, Davood; Ghasemi-Nejhad, Mehrdad N

2012-01-01

The main objective is to improve the most commonly addressed weakness of the laminated composites (i.e. delamination due to poor interlaminar strength) using carbon nanotubes (CNTs) as reinforcement between the laminae and in the transverse direction. In this work, a chemical vapor deposition technique has been used to grow dense vertically aligned arrays of CNTs over the surface of chemically treated two-dimensionally woven cloth and fiber tows. The nanoforest-like fabrics can be used to fabricate three-dimensionally reinforced laminated nanocomposites. The presence of CNTs aligned normal to the layers and in-between the layers of laminated composites is expected to considerably enhance the properties of the laminates. To demonstrate the effectiveness of our approach, composite single lap-joint specimens were fabricated for interlaminar shear strength testing. It was observed that the single lap-joints with through-the-thickness CNT reinforcement can carry considerably higher shear stresses and strains. Close examination of the test specimens showed that the failure of samples with CNT nanoforests was completely cohesive, while the samples without CNT reinforcement failed adhesively. This concludes that the adhesion of adjacent carbon fabric layers can be considerably improved owing to the presence of vertically aligned arrays of CNT nanoforests. PMID:27877502

Laser welded steel sandwich panel bridge deck development : finite element analysis and stake weld strength tests.

DOT National Transportation Integrated Search

2009-09-01

This report summarizes the analysis of laser welded steel sandwich panels for use in bridge structures and : static testing of laser stake welded lap shear coupons. Steel sandwich panels consist of two face sheets : connected by a relatively low-dens...

Toughening of Epoxy Adhesives by Combined Interaction of Carbon Nanotubes and Silsesquioxanes

PubMed Central

Barra, Giuseppina; Vertuccio, Luigi; Vietri, Umberto; Naddeo, Carlo; Guadagno, Liberata

2017-01-01

The extensive use of adhesives in many structural applications in the transport industry and particularly in the aeronautic field is due to numerous advantages of bonded joints. However, still many researchers are working to enhance the mechanical properties and rheological performance of adhesives by using nanoadditives. In this study the effect of the addition of Multi-Wall Carbon Nanotubes (MWCNTs) with Polyhedral Oligomeric Silsesquioxane (POSS) compounds, either Glycidyl Oligomeric Silsesquioxanes (GPOSS) or DodecaPhenyl Oligomeric Silsesquioxanes (DPHPOSS) to Tetraglycidyl Methylene Dianiline (TGMDA) epoxy formulation, was investigated. The formulations contain neither a tougher matrix such as elastomers nor other additives typically used to provide a closer match in the coefficient of thermal expansion in order to discriminate only the effect of the addition of the above-mentioned components. Bonded aluminium single lap joints were made using both untreated and Chromic Acid Anodisation (CAA)-treated aluminium alloy T2024 adherends. The effects of the different chemical functionalities of POSS compounds, as well as the synergistic effect between the MWCNT and POSS combination on adhesion strength, were evaluated by viscosity measurement, tensile tests, Dynamic Mechanical Analysis (DMA), single lap joint shear strength tests, and morphological investigation. The best performance in the Lap Shear Strength (LSS) of the manufactured joints has been found for treated adherends bonded with epoxy adhesive containing MWCNTs and GPOSS. Carbon nanotubes have been found to play a very effective bridging function across the fracture surface of the bonded joints. PMID:28946691

SEM/XPS analysis of fractured adhesively bonded graphite fibre-reinforced polyimide composites

NASA Technical Reports Server (NTRS)

Devilbiss, T. A.; Messick, D. L.; Wightman, J. P.; Progar, D. J.

1985-01-01

The surfaces of the graphite fiber-reinforced polyimide composites presently pretreated prior to bonding with polyimide adhesive contained variable amounts of a fluoropolymer, as determined by X-ray photoelectron spectroscopy. Lap shear strengths were determined for unaged samples and for those aged over 500- and 1000-hour periods at 177 and 232 C. Unaged sample lap strengths, which were the highest obtained, exhibited no variation with surface pretreatment, but a significant decrease is noted with increasing aging temperature. These thermally aged samples, however, had increased surface fluorine concentration, while a minimal concentration was found in unaged samples. SEM demonstrated a progressive shift from cohesive to adhesive failure for elevated temperature-aged composites.

Steady-state sinusoidal behavior of elastomeric dampers

NASA Astrophysics Data System (ADS)

Madhavan, Vijay; Wereley, Norman M.; Sieg, Thierry

1999-06-01

This paper presents an experimental and analytical investigation of an elastomeric damping material and assesses its potential application to stability augmentation of hingeless and bearingless helicopter rotors. Double lap shear specimens were tested on a servo-hydraulic testing machine. Single frequency sinusoidal tests were conducted over a strain amplitude range of 0 - 30% at three frequencies (lag/rev, 1/rev and a lower harmonic of the rotor). The frequencies were chosen such that the effect of the damper in mitigating instability phenomena, like ground and air resonance, could be analyzed. The effects of frequency, amplitude, pre-load and material self-heating were studied. A three-element mechanisms-based damper model was developed that accurately captures the energy dissipation and hysteresis behavior of the damper. The model incorporates a linear stiffness, viscous damping and a non-linear slip element that are placed in parallel to each other. The parameters of the model were identified using an LMS technique. The model was validated by reconstructing measured hysteresis cycles using these parameters.

Adhesive evaluation of new polyimides

NASA Technical Reports Server (NTRS)

Stclair, Terry L.; Progar, Donald J.

1987-01-01

During the past 10 to 15 years, the Materials Division at NASA Langley Research Center (LaRC) has developed several novel high temperature polyimide adhesives for anticipated needs of the aerospace industry. These developments have resulted from fundamental studies of structure-property relationships in polyimides. Recent research at LaRC has involved the synthesis and evaluation of copolyimides which incorporate both flexibilizing bridging groups and meta-linked benzene rings. The purpose was to develop systems based on low cost, readily available monomers. Two of these copolyimides evaluated as adhesives for bonding titanium alloy, Ti(6Al-4V), are identified as LARC-STPI and STPI-LARC-2. Lap shear strength (LSS) measurements were used to determine the strength and durability of the adhesive materials. LSS results are presented for LARC-TPI and LARC-STPI lap shear specimens thermally exposed in air at 232 C for up to 5000 hrs. LARC-TPI was shown to perform better than the copolymer LARC-STPI which exhibited poor thermooxidative performance possibly due to the amines used which would tend to oxidize easier than the benzophenone system in LARC-TPI.

Induction of polyploidy by nuclear fusion mechanism upon decreased expression of the nuclear envelope protein LAP2β in the human osteosarcoma cell line U2OS.

PubMed

Ben-Shoshan, Shirley Oren; Simon, Amos J; Jacob-Hirsch, Jasmine; Shaklai, Sigal; Paz-Yaacov, Nurit; Amariglio, Ninette; Rechavi, Gideon; Trakhtenbrot, Luba

2014-01-28

Polyploidy has been recognized for many years as an important hallmark of cancer cells. Polyploid cells can arise through cell fusion, endoreplication and abortive cell cycle. The inner nuclear membrane protein LAP2β plays key roles in nuclear envelope breakdown and reassembly during mitosis, initiation of replication and transcriptional repression. Here we studied the function of LAP2β in the maintenance of cell ploidy state, a role which has not yet been assigned to this protein. By knocking down the expression of LAP2β, using both viral and non-viral RNAi approaches in osteosarcoma derived U2OS cells, we detected enlarged nuclear size, nearly doubling of DNA content and chromosomal duplications, as analyzed by fluorescent in situ hybridization and spectral karyotyping methodologies. Spectral karyotyping analyses revealed that near-hexaploid karyotypes of LAP2β knocked down cells consisted of not only seven duplicated chromosomal markers, as could be anticipated by genome duplication mechanism, but also of four single chromosomal markers. Furthermore, spectral karyotyping analysis revealed that both of two near-triploid U2OS sub-clones contained the seven markers that were duplicated in LAP2β knocked down cells, whereas the four single chromosomal markers were detected only in one of them. Gene expression profiling of LAP2β knocked down cells revealed that up to a third of the genes exhibiting significant changes in their expression are involved in cancer progression. Our results suggest that nuclear fusion mechanism underlies the polyploidization induction upon LAP2β reduced expression. Our study implies on a novel role of LAP2β in the maintenance of cell ploidy status. LAP2β depleted U2OS cells can serve as a model to investigate polyploidy and aneuploidy formation by nuclear fusion mechanism and its involvement in cancerogenesis.

High Temperature Adhesives for Bonding Kapton

NASA Technical Reports Server (NTRS)

Stclair, A. K.; Slemp, W. S.; Stclair, T. L.

1978-01-01

Experimental polyimide resins were developed and evaluated as potential high temperature adhesives for bonding Kapton polyimide film. Lap shear strengths of Kapton/Kapton bonds were obtained as a function of test temperature, adherend thickness, and long term aging at 575K (575 F) in vacuum. Glass transition temperatures of the polyimide/Kapton bondlines were monitored by thermomechanical analysis.

High temperature adhesives for bonding Kapton

NASA Technical Reports Server (NTRS)

Saint Clair, A. K.; Slemp, W. S.; Saint Clair, T. L.

1978-01-01

Experimental polyimide resins have been developed and evaluated as potential high temperature adhesives for bonding Kapton polyimide film. Lap shear strengths of 'Kapton'/'Kapton' bonds were obtained as a function of test temperature, adherend thickness, and long term aging at 575K (575 F) in vacuum. Glass transition temperatures of the polyimide/'Kapton' bondlines were monitored by thermomechanical analysis.

On double shearing in frictional materials

NASA Astrophysics Data System (ADS)

Teunissen, J. A. M.

2007-01-01

This paper evaluates the mechanical behaviour of yielding frictional geomaterials. The general Double Shearing model describes this behaviour. Non-coaxiality of stress and plastic strain increments for plane strain conditions forms an important part of this model. The model is based on a micro-mechanical and macro-mechanical formulation. The stress-dilatancy theory in the model combines the mechanical behaviour on both scales.It is shown that the general Double Shearing formulation comprises other Double Shearing models. These models differ in the relation between the mobilized friction and dilatancy and in non-coaxiality. In order to describe reversible and irreversible deformations the general Double Shearing model is extended with elasticity.The failure of soil masses is controlled by shear mechanisms. These shear mechanisms are determined by the conditions along the shear band. The shear stress ratio of a shear band depends on the orientation of the stress in the shear band. There is a difference between the peak strength and the residual strength in the shear band. While peak stress depends on strength properties only, the residual strength depends upon the yield conditions and the plastic deformation mechanisms and is generally considerably lower than the maximum strength. It is shown that non-coaxial models give non-unique solutions for the shear stress ratio on the shear band. The Double Shearing model is applied to various failure problems of soils such as the direct simple shear test, the biaxial test, infinite slopes, interfaces and for the calculation of the undrained shear strength. Copyright

Solid-state and fusion resistance spot welding of TD-NiCr sheet

NASA Technical Reports Server (NTRS)

Moore, T. J.

1973-01-01

By using specially processed TD-NiCr sheet in both 0.4-mm (0.015-in.) and 1.6-mm (0.062-in.) thicknesses and carefully selected welding procedures, solid state resistance spot welds were produced which, after postheating at 1200 C, were indistinguishable from the parent material. Stress-rupture shear tests of single-spot lap joints in 0.4-mm (0.015-in.) thick sheet showed that these welds were as strong as the parent material. Similar results were obtained in tensile-shear tests at room temperature and 1100 C and in fatigue tests. Conventional fusion spot welds in commercial sheet were unsatisfactory because of poor stress-rupture shear properties resulting from metallurgical damage to the parent material.

Warrior Injury Assessment Manikin (WIAMan) Lumbar Spine Model Validation: Development, Testing, and Analysis of Physical and Computational Models of the WIAMan Lumbar Spine Materials Demonstrator

DTIC Science & Technology

2016-08-01

load. The 1 and 10 s-1 rate tests were run on a hydraulic high-rate Instron MTS (8821S), placed in a custom- designed tension fixture (Fig. 8...lateral compression prior to shear testing . The sides of the coupon rest on blocks at the bottom of the vice jaw to allow for travel of the center post ...mode of failure based on the lap shear testing . However, since the pretest spine survived all hits at the BRC speeds, it was decided to proceed with

Viscoelastic analysis of adhesively bonded joints

NASA Technical Reports Server (NTRS)

Delale, F.; Erdogan, F.

1980-01-01

An adhesively bonded lap joint is analyzed by assuming that the adherends are elastic and the adhesive is linearly viscoelastic. After formulating the general problem a specific example for two identical adherends bonded through a three parameter viscoelastic solid adhesive is considered. The standard Laplace transform technique is used to solve the problem. The stress distribution in the adhesive layer is calculated for three different external loads, namely, membrane loading, bending, and transverse shear loading. The results indicate that the peak value of the normal stress in the adhesive is not only consistently higher than the corresponding shear stress but also decays slower.

Self-monitoring fiber reinforced polymer strengthening system for civil engineering infrastructures

NASA Astrophysics Data System (ADS)

Jiang, Guoliang; Dawood, Mina; Peters, Kara; Rizkalla, Sami

2008-03-01

Fiber reinforced polymer (FRP) materials are currently used for strengthening civil engineering infrastructures. The strengthening system is dependant on the bond characteristics of the FRP to the external surface of the structure to be effective in resisting the applied loads. This paper presents an innovative self-monitoring FRP strengthening system. The system consists of two components which can be embedded in FRP materials to monitor the global and local behavior of the strengthened structure respectively. The first component of the system is designed to evaluate the applied load acting on a structure based on elongation of the FRP layer along the entire span of the structure. Success of the global system has been demonstrated using a full-scale prestressed concrete bridge girder which was loaded up to failure. The test results indicate that this type of sensor can be used to accurately determine the load prior to failure within 15 percent of the measured value. The second sensor component consists of fiber Bragg grating sensors. The sensors were used to monitor the behavior of steel double-lap shear splices tested under tensile loading up to failure. The measurements were used to identify abnormal structural behavior such as epoxy cracking and FRP debonding. Test results were also compared to numerical values obtained from a three dimensional shear-lag model which was developed to predict the sensor response.

Morphing hybrid honeycomb (MOHYCOMB) with in situ Poisson’s ratio modulation

NASA Astrophysics Data System (ADS)

Heath, Callum J. C.; Neville, Robin M.; Scarpa, Fabrizio; Bond, Ian P.; Potter, Kevin D.

2016-08-01

Electrostatic adhesion can be used as a means of reversible attachment. Through application of high voltage (~2 kV) across closely spaced parallel plate electrodes, significant shear stresses (11 kPa) can be generated. The highest levels of electrostatic holding force can be achieved through close contact of connection surfaces; this is facilitated by flexible electrodes which can conform to reduce air gaps. Cellular structures are comprised of thin walled elements, making them ideal host structures for electrostatic adhesive elements. The reversible adhesion provides control of the internal connectivity of the cellular structure, and determines the effective cell geometry. This would offer variable stiffness and control of the effective Poisson’s ratio of the global cellular array. Using copper-polyimide thin film laminates and PVDF thin film dielectrics, double lap shear electrostatic adhesive elements have been introduced to a cellular geometry. By activating different groups of reversible adhesive interfaces, the cellular array can assume four different cell configurations. A maximum stiffness modulation of 450% between the ‘All off’ and ‘All on’ cell morphologies has been demonstrated. This structure is also capable of in situ effective Poisson’s ratio variations, with the ability to switch between values of -0.45 and 0.54. Such a structure offers the potential for tuneable vibration absorption (due to its variable stiffness properties), or as a smart honeycomb with controllable curvature and is termed morphing hybrid honeycomb.

Analytical and experimental study of the vibration of bonded beams with a lap joint

NASA Technical Reports Server (NTRS)

Rao, M. D.; Crocker, M. J.

1990-01-01

A theoretical model to study the flexural vibration of a bonded lap joint system is described in this paper. First, equations of motion at the joint region are derived using a differential element approach. The transverse displacements of the upper and lower beam are considered to be different. The adhesive is assumed to be linearly viscoelastic and the widely used Kelvin-Voight model is used to represent the viscoelastic behavior of the adhesive. The shear force at the interface between the adhesive and the beam is obtained from the simple bending motion equations of the two beams. The resulting equations of motion are combined with the equations of transverse vibration of the beams in the unjointed regions. These are later solved as a boundary value problem to obtain the eigenvalues and eigenvectors of the system. The model can be used to predict the natural frequencies, modal damping ratios, and mode shapes of the system for free vibration. Good agreement between numerical and experimental results was obtained for a system of graphite epoxy beams lap-jointed by an epoxy adhesive.

High-temperature adhesives for bonding polyimide film. [bonding Kapton film for solar sails

NASA Technical Reports Server (NTRS)

St.clair, A. K.; Slemp, W. S.; St.clair, T. L.

1980-01-01

Experimental polyimide resins were developed and evaluated as potential high temperature adhesives for bonding Kapton polyimide film. Lap shear strengths of Kapton/Kapton bonds were obtained as a function of test temperature, adherend thickness, and long term aging at 575 K (575 F) in vacuum. Glass transition temperatures of the polyimide/"Kapton" bondlines were monitored by thermomechanical analysis.

Temperature Effects on Adhesive Bond Strengths and Modulus for Commonly Used Spacecraft Structural Adhesives

NASA Technical Reports Server (NTRS)

Ojeda, Cassandra E.; Oakes, Eric J.; Hill, Jennifer R.; Aldi, Dominic; Forsberg, Gustaf A.

2011-01-01

A study was performed to observe how changes in temperature and substrate material affected the strength and modulus of an adhesive bondline. Seven different adhesives commonly used in aerospace bonded structures were tested. Aluminum, titanium and Invar adherends were cleaned and primed, then bonded using the manufacturer's recommendations. Following surface preparation, the coupons were bonded with the adhesives. The single lap shear coupons were then pull tested per ASTM D 1002 Standard Test Method for Apparent Shear Strength of Single- Lap-Joint over a temperature range from -150 deg C up to +150 deg C. The ultimate strength was calculated and the resulting data were converted into B-basis design allowables. Average and Bbasis results were compared. Results obtained using aluminum adherends are reported. The effects of using different adherend materials and temperature were also studied and will be reported in a subsequent paper. Dynamic Mechanical Analysis (DMA) was used to study variations in adhesive modulus with temperature. This work resulted in a highly useful database for comparing adhesive performance over a wide range of temperatures, and has facilitated selection of the appropriate adhesive for spacecraft structure applications.

A test of the double-shearing model of flow for granular materials

USGS Publications Warehouse

Savage, J.C.; Lockner, D.A.

1997-01-01

The double-shearing model of flow attributes plastic deformation in granular materials to cooperative slip on conjugate Coulomb shears (surfaces upon which the Coulomb yield condition is satisfied). The strict formulation of the double-shearing model then requires that the slip lines in the material coincide with the Coulomb shears. Three different experiments that approximate simple shear deformation in granular media appear to be inconsistent with this strict formulation. For example, the orientation of the principal stress axes in a layer of sand driven in steady, simple shear was measured subject to the assumption that the Coulomb failure criterion was satisfied on some surfaces (orientation unspecified) within the sand layer. The orientation of the inferred principal compressive axis was then compared with the orientations predicted by the double-shearing model. The strict formulation of the model [Spencer, 1982] predicts that the principal stress axes should rotate in a sense opposite to that inferred from the experiments. A less restrictive formulation of the double-shearing model by de Josselin de Jong [1971] does not completely specify the solution but does prescribe limits on the possible orientations of the principal stress axes. The orientations of the principal compression axis inferred from the experiments are probably within those limits. An elastoplastic formulation of the double-shearing model [de Josselin de Jong, 1988] is reasonably consistent with the experiments, although quantitative agreement was not attained. Thus we conclude that the double-shearing model may be a viable law to describe deformation of granular materials, but the macroscopic slip surfaces will not in general coincide with the Coulomb shears.

Delamination failure of multilaminated adhesively bonded joints at low temperatures

NASA Astrophysics Data System (ADS)

Lee, Chi-Seung; Chun, Min-Sung; Kim, Myung-Hyun; Lee, Jae-Myung

2011-08-01

A series of experimental investigations of multilaminated joints adhesively bonded by epoxy/polyurethane (PU) glue were conducted in order to examine the delamination failure characteristics under in-plane shear loading at low temperatures. In order to observe these phenomena, a series of lap-shear tests were carried out at various low temperatures (20 °C, -110 °C and -163 °C) and various adhesion areas (15 mm × 50 mm, 30 mm × 50 mm, 50 mm × 50 mm, 75 mm × 50 mm and 100 mm × 50 mm). The test results were used to investigate the delamination and material characteristics, as well as the material properties, e.g., ultimate shear stress and shear elongation. Furthermore, the dependencies of the characteristics of multilaminated adhesively bonded joints (MABJs) on temperature and adhesion area was analyzed using the stress-strain relationship, and closed form formulas that are functions of the dependent parameters are proposed.

Non-destructive Evaluation of Bonds Between Fiberglass Composite and Metal

NASA Technical Reports Server (NTRS)

Zhao, Selina; Sonta, Kestutis; Perey, Daniel F.; Cramer, K. E.; Berger, Libby

2015-01-01

To assess the integrity and reliability of an adhesive joint in an automotive composite component, several non-destructive evaluation (NDE) methodologies are correlated to lap shear bond strengths. A glass-fabric-reinforced composite structure was bonded to a metallic structure with a two-part epoxy adhesive. Samples were subsequently cut and tested in shear, and flaws were found in some areas. This study aims to develop a reliable and portable NDE system for service-level adhesive inspection in the automotive industry. The results of the experimental investigation using several NDE methods are presented and discussed. Fiberglass-to-metal bonding is the ideal configuration for NDE via thermography using excitation with induction heating, due to the conductive metal and non-conductive glass-fiber-reinforced composites. Excitation can be either by a research-grade induction heater of highly defined frequency and intensity, or by a service-level heater, such as would be used for sealing windshields in a body shop. The thermographs thus produced can be captured via a high-resolution infrared camera, with principal component analysis and 2D spatial Laplacian processing. Alternatively, the thermographs can be captured by low resolution thermochromic microencapsulated liquid crystal film imaging, which needs no post-processing and can be very inexpensive. These samples were also examined with phased-array ultrasound. The NDE methods are compared to the lap shear values and to each other for approximate cost, accuracy, and time and level of expertise needed.

Optimum design of bolted composite lap joints under mechanical and thermal loading

NASA Astrophysics Data System (ADS)

Kradinov, Vladimir Yurievich

A new approach is developed for the analysis and design of mechanically fastened composite lap joints under mechanical and thermal loading. Based on the combined complex potential and variational formulation, the solution method satisfies the equilibrium equations exactly while the boundary conditions are satisfied by minimizing the total potential. This approach is capable of modeling finite laminate planform dimensions, uniform and variable laminate thickness, laminate lay-up, interaction among bolts, bolt torque, bolt flexibility, bolt size, bolt-hole clearance and interference, insert dimensions and insert material properties. Comparing to the finite element analysis, the robustness of the method does not decrease when modeling the interaction of many bolts; also, the method is more suitable for parametric study and design optimization. The Genetic Algorithm (GA), a powerful optimization technique for multiple extrema functions in multiple dimensions search spaces, is applied in conjunction with the complex potential and variational formulation to achieve optimum designs of bolted composite lap joints. The objective of the optimization is to acquire such a design that ensures the highest strength of the joint. The fitness function for the GA optimization is based on the average stress failure criterion predicting net-section, shear-out, and bearing failure modes in bolted lap joints. The criterion accounts for the stress distribution in the thickness direction at the bolt location by applying an approach utilizing a beam on an elastic foundation formulation.

Influence of laser beam incidence angle on laser lap welding quality of galvanized steels

NASA Astrophysics Data System (ADS)

Mei, Lifang; Yan, Dongbing; Chen, Genyu; Wang, Zhenhui; Chen, Shuixuan

2017-11-01

Based on the characteristics of laser welded structural parts of auto bodies, the influence of variation in laser beam incidence angle on the lap welding performance of galvanized auto-body sheets was studied. Lap welding tests were carried out on the galvanized sheets for auto-body application at different laser beam incidence angles by using the optimal welding parameters obtained through orthogonal experiment. The effects of incidence angle variation on seam appearance, cross-sectional shape, joint mechanical properties and microstructure of weldments were analyzed. In addition, the main factors influencing the value of incidence angle were investigated. According to the results, the weld seams had a good appearance as well as a fine, and uniform microstructure when the laser beam incidence angle was smaller than the critical incidence angle, and thus they could withstand great tensile and shear loads. Moreover, all tensile-shear specimens were fractured in the base material zone. When the laser beam incidence angle was larger than the critical incidence angle, defects like shrinkage and collapse tended to emerge, thereby resulting in the deteriorated weldability of specimens. Meanwhile, factors like the type and thickness of sheet, weld width as well as inter-sheet gap all had a certain effect on the value of laser beam incidence angle. When the sheet thickness was small and the weld width was narrow, the laser beam incidence angle could be increased appropriately. At the same time, small changes in the inter-sheet gap could greatly impact the value of incidence angle. When the inter-sheet gap was small, the laser beam incidence angle should not be too large.

Viscoelastic analysis of adhesively bonded joints

NASA Technical Reports Server (NTRS)

Delale, F.; Erdogan, F.

1981-01-01

In this paper an adhesively bonded lap joint is analyzed by assuming that the adherends are elastic and the adhesive is linearly viscoelastic. After formulating the general problem a specific example for two identical adherends bonded through a three parameter viscoelastic solid adhesive is considered. The standard Laplace transform technique is used to solve the problem. The stress distribution in the adhesive layer is calculated for three different external loads namely, membrane loading, bending, and transverse shear loading. The results indicate that the peak value of the normal stress in the adhesive is not only consistently higher than the corresponding shear stress but also decays slower.

Development of acceptance criteria for batches of silane primer for external tank thermal protection system bonding applications

NASA Technical Reports Server (NTRS)

Mikes, F.

1985-01-01

Concluding tests for the thermogravimetric and FTIR analyses of DC 1200 silane primers are discussed as well as methods for HPLC and GC analyses and for determining titanium and silicon by atomic absorption spectroscopy. Tables summarizes results obtained for residue, ash, titanium, silicone, Si/Ti ratio, OH-absorption, the lap-shear test, and the GC headspace for alcohols.

Three-dimensional finite element analyses of the local mechanical behavior of riveted lap joints

NASA Astrophysics Data System (ADS)

Iyer, Kaushik Arjunan

Three-dimensional elastic-plastic finite element models of single and double rivet-row lap joints have been developed to evaluate local distortions and the mechanics of airframe-type 7075-T6 aluminum alloy riveted assemblies. Loading induced distortion features such as the excess assembly compliance, rivet tilt, local in- and out-of-plane slips and stress concentration factors are evaluated as functions of rivet countersinking, rivet material and friction coefficient. Computed features are examined to identify alterations in the proportions of in-plane and out-of-plane load transmission across rivet-panel interfaces and isolate global and lower-order effects present in the complex response of these multi-body assemblies. Analytical procedures are validated by comparing calculated and measured values of excess assembly compliance and local panel bending. Direct out-of-plane load transmission between the rivet heads and panels affects global deformation features such as remote panel bending and local features such as the panel stress concentration factor. The increase in stress concentration due to panel bending is self-limiting owing to decreasing in-plane load bearing with increasing rivet tilt, which is a composite reflection of the basic rivet deformation modes of shear and rotation. Calculations have also been performed to define approximate steady-state fretting fatigue conditions that lead to crack initiation at a panel hole surface in single and double rivet-row assemblies for countersunk and non-countersunk rivets. These account for and isolate effects of interference and clamping forces on fatigue performance by comparing computed circumferential variations of bulk residual stresses, cyclic stress range and mean stress. With interference, a non-countersunk assembly is shown to be as prone to crack initiation as a countersunk assembly. Frictional work due to fretting is evaluated and the physical location of fretting fatigue crack initiation is predicted by interpreting combined effects of primary fretting fatigue parameters such as contact pressure and slip amplitude. Angular shifts in the peaks of conventional fatigue and fretting fatigue parameters caused by interference may be exploited to optimize the residual life of aging airframes.

Deformations and strains in adhesive joints by moire interferometry

NASA Technical Reports Server (NTRS)

Post, D.; Czarnek, R.; Wood, J.; John, D.; Lubowinski, S.

1984-01-01

Displacement fields in a thick adherend lap joint and a cracked lap shear specimen were measured by high sensitivity moire interferometry. Contour maps of in-plane U and V displacements were obtained across adhesive and adherent surfaces. Loading sequences ranged from modest loads to near-failure loads. Quantitative results are given for displacements and certain strains in the adhesive and along the adhesive/adherend boundary lines. The results show nonlinear displacements and strains as a function of loads or stresses and they show viscoelastic or time-dependent response. Moire interferometry is an excellent method for experimental studies of adhesive joint performance. Subwavelength displacement resolution of a few micro-inches, and spatial resolution corresponding to 1600 fringes/inch (64 fringes/mm), were obtained in these studies. The whole-field contour maps offer insights not available from local measurements made by high sensitivity gages.

Method for computing energy release rate using the elastic work factor approach

NASA Astrophysics Data System (ADS)

Rhee, K. Y.; Ernst, H. A.

1992-01-01

The elastic work factor eta(el) concept was applied to composite structures for the calculation of total energy release rate by using a single specimen. Cracked lap shear specimens with four different unidirectional fiber orientation were used to examine the dependence of eta(el) on the material properties. Also, three different thickness ratios (lap/strap) were used to determine how geometric conditions affect eta(el). The eta(el) values were calculated in two different ways: compliance method and crack closure method. The results show that the two methods produce comparable eta(el) values and, while eta(el) is affected significantly by geometric conditions, it is reasonably independent of material properties for the given geometry. The results also showed that the elastic work factor can be used to calculate total energy release rate using a single specimen.

Investigations on Laser Beam Welding of Different Dissimilar Joints of Steel and Aluminum Alloys for Automotive Lightweight Construction

NASA Astrophysics Data System (ADS)

Seffer, Oliver; Pfeifer, Ronny; Springer, André; Kaierle, Stefan

Due to the enormous potential of weight saving, and the consequential reduction of pollutant emissions, the use of hybrid components made of steel and aluminum alloys is increasing steadily, especially concerning automotive lightweight construction. However, thermal joining of steel and aluminum is still being researched, due to a limited solubility of the binary system of iron and aluminum causing the formation of hard and brittle intermetallic phases, which decrease the strength and the formability of the dissimilar seam. The presented results show the investigation of laser beam welding for joining different dissimilar hybrid components of the steel materials HX220LAD+Z100, 22MnB5+AS150 and 1.4301, as well as the aluminum alloy AA6016-T4 as a lap joint. Among other things, the influences of the energy per unit length, the material grade, the sheet thickness t, the weld type (lap weld, fillet weld) and the arrangement of the base materials in a lap joint (aluminum-sided irradiation, steel-sided irradiation) on the achievable strengths are analyzed. The characterization of the dissimilar joints includes tensile shear tests and metallographic analyses, depending on the energy per unit length.

Amplification in Double Heterostructure GaAs Lasers.

DTIC Science & Technology

1981-03-15

done, for example, in the book by Siegman . When the laser signal which is to be amplified is a single mode, it is important to include the possibility...k A’AD-A097 862 AEROSPACE CORP EL SEGUNDO CA ELECTRONICS RESEARCH LAP) P 5 20/5 I AMPLIFICATION IN DOUBLE HETEROSTRUCTURE GAAS LASERS .(U IMAR al E...GARMIRE, M CHANG F04701-80-C-0081I UNCLASSIFIED TR GO81(6930 03)-2 SD-TA8-30 NL Amplification in Double Heterostructure GaAs Lasers E. GARMIRE nd M

The Effects of Temperature, Humidity and Aircraft Fluid Exposure on T800H/3900-2 Composites Bonded with AF-555M Adhesive

NASA Technical Reports Server (NTRS)

Miner, Gilda A.; Hou, Tan-Hung; Lowther, Sharon E.; Thibeault, Sheila A.; Connell, John W.; Blasini, Sheila Roman

2010-01-01

Fiber reinforced resin matrix composites and structural adhesives have found increased usage on commercial and military aircraft in recent years. Due to the lack of service history of these relatively new material systems, their long-term aging performance has not been well established. In this study, single lap shear specimens (SLS) were fabricated by secondary bonding of Scotch-Weld(TradeMark) AF-555M between pre-cured adherends comprised of T800H/3900-2 uni-directional laminates. The adherends were co-cured with wet peel-ply for surface preparation. Each bond-line of the SLS specimen was measured to determine thickness and inspected visually using an optical microscope for voids. A three-year environmental aging plan for the SLS specimens at 82 C (180 F) and 85% relative humidity was initiated. SLS strengths were measured for both controls and aged specimens at room temperature and 82 C. The effect of this exposure on lap shear strength and failure modes to date is reported. In addition, the effects of water, saline water, deicing fluid, JP-5 jet fuel and hydraulic fluid on both the composite material and the adhesive bonds were investigated. The up to date results on the effects of these exposures will be discussed.

Ultrasonic Spot Welding of a Rare-Earth Containing ZEK100 Magnesium Alloy: Effect of Welding Energy

NASA Astrophysics Data System (ADS)

Macwan, A.; Chen, D. L.

2016-04-01

Ultrasonic spot welding was used to join a low rare-earth containing ZEK100 Mg alloy at different levels of welding energy, and tensile lap shear tests were conducted to evaluate the failure strength in relation to the microstructural changes. It was observed that dynamic recrystallization occurred in the nugget zone; the grain size increased and microhardness decreased with increasing welding energy arising from the increasing interface temperature and strain rate. The weld interface experienced severe plastic deformation at a high strain rate from ~500 to ~2100 s-1 with increasing welding energy from 500 to 2000 J. A relationship between grain size and Zener-Hollomon parameter, and a Hall-Petch-type relationship between microhardness and grain size were established. The tensile lap shear strength and failure energy were observed to first increase with increasing welding energy, reach the maximum values at 1500 J, and then decrease with a further increase in the welding energy. The samples welded at a welding energy ≤1500 J exhibited an interfacial failure mode, while nugget pull-out occurred in the samples welded at a welding energy above 1500 J. The fracture surfaces showed typical shear failure. Low-temperature tests at 233 K (-40 °C) showed no significant effect on the strength and failure mode of joints welded at the optimal welding energy of 1500 J. Elevated temperature tests at 453 K (180 °C) revealed a lower failure load but a higher failure energy due to the increased deformability, and showed a mixed mode of partial interfacial failure and partial nugget pull-out.

A fundamental approach to adhesion: Synthesis, surface analysis, thermodynamics and mechanics

NASA Technical Reports Server (NTRS)

Dwight, D. W.; Wightman, J. P.

1977-01-01

The ability of SEM/EDAX to determine the physical and chemical composition of very small areas was used to study several diverse types of samples representative of NASA-LaRC technology. More systematic investigation was carried out on differences in the results of grit-blasting Ti 6-4 adherends and the presence of extraneous elements, primarily silicon, in some polymer/HT-S fiber composites. Initial results were obtained from a fractured (ILS) short-beam shear specimen, and from Ti 6-4 alloy, before and after a proprietary Boeing anodizing surface preparation for adhesive bonding. Photomicrographs and EDAX spectra were also obtained from new, fractured lap shear strength specimens that employed PPQ and LARC-13 adhesives.

Effect of interactions between multiple interfaces on the rheological characteristics of double emulsions

NASA Astrophysics Data System (ADS)

Choi, Se Bin; Park, Jae Yong; Moon, Ji Young; Lee, Joon Sang

2018-06-01

In this study, we analyzed the rheological characteristics of double emulsions by using a three-dimensional lattice Boltzmann model. Numerical simulations indicate that interactions between multiple interfaces play a vital role in determining the shear stress on interfaces and affect deformations, which influence the relative viscosity of double emulsions. The large shear stress induced by droplets in contact increases the relative viscosity for high volume fractions. The double emulsions also show shear-thinning behavior, which corresponds with the Carreau model. The interfacial interference between the core and the deforming shell cause the relative viscosity to increase with increasing core-droplet radius. Finally, we investigated the dependence of the double-emulsion viscosity on the core-droplet viscosity. At high shear rates, the relative viscosity increases with increasing core-droplet viscosity. However, the trend is opposite at low shear rates, which results from the high inward flow (Marangoni flow) at low core-droplet viscosity.

The cyclic fatigue behavior of adhesive joints

NASA Astrophysics Data System (ADS)

Kinloch, A. J.; Toh, T.

1995-06-01

In the last six months we have: (1) Concentrated our efforts on the fatigue failure of carbon-fiber PEEK/AFl63 lap joints, and in particular we have started to predict the life time of single-lap joints under cyclic fatigue loading. The analysis is based on data obtained from double cantilever beam (DCB) fracture mechanics tests; (2) Further, we have been successful in measuring the rate of crack growth in lap joints during fatigue fracture using ultrasonic scanning; (3) Preliminary test data on the static fracture of glass-fiber reinforced poly(phenylene sulphide) (PPS)/AF163 joints have also been studied; and (4) A comparison has been made in computing the critical strain energy release rate G(sub c) for the glass-fiber PPS/AF163 joints based on the compliance method, beam theory and corrected beam theory. The last method accounts for large non-linear deflections and the associated crack root rotations along with the necessary corrections for the increase in stiffness introduced by the presence of end blocks.

Friction stir scribe welding technique for dissimilar joining of aluminium and galvanised steel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Tianhao; Sidhar, Harpreet; Mishra, Rajiv S.

Friction stir scribe technology, a derivative of friction stir welding, was applied for the dissimilar lap welding of an aluminum alloy and galvanized mild steel sheets. During the process, the rotating tool with a cobalt steel scribe first penetrated the top material — aluminum — and then the scribe cut the bottom material — steel. The steel was displaced into the upper material to produce a characteristic hook feature. Lap welds were shear tested, and their fracture paths were studied. Welding parameters affected the welding features including hook height, which turned out to be highly related to fracture position. Therefore,more » in this paper, the relationships among welding parameters, hook height, joint strength and fracture position are presented. In addition, influence of zinc coating on joint strength was also studied. Keywords: friction stir scribe technology; dissimilar material welding; zinc coating; hook height; joint strength; fracture position« less

Advanced joining concepts for passive vibration control

NASA Technical Reports Server (NTRS)

Prucz, Jacky C.; Spyrakos, Constantine

1987-01-01

A comprehensive parametric study was carried out to establish design guidelines for favorable tradeoffs between damping benefits and the associated stiffness, strength and weight penalties in a rhombic joint. The results are compared with the corresponding tradeoffs for a double-lap joint made of the same materials.

The role of peel stresses in cyclic debonding

NASA Technical Reports Server (NTRS)

Everett, R. A., Jr.

1982-01-01

When an adhesively bonded joint is undergoing cyclic loading, one of the possible damage modes that occurs is called cyclic debonding - progressive separation of the adherends by failure of the adhesive bond under cyclic loading. In most practical structures, both peel and shear stresses exist in the adhesive bonding during cyclic loading. The results of an experimental and analytical study to determine the role of peel stresses on cyclic debonding in a mixed mode specimen are presented. Experimentally, this was done by controlling the forces that create the peel stresses by applying a clamping force to oppose the peel stresses. Cracked lap shear joints were chosen for this study. A finite element analysis was developed to assess the effect of the clamping force on the strain energy release rates due to shear and peel stresses. The results imply that the peel stress is the principal stress causing cyclic debonding.

Gender-specific contribution of cardiometabolic index and lipid accumulation product to left ventricular geometry change in general population of rural China.

PubMed

Wang, Haoyu; Sun, Yingxian; Li, Zhao; Guo, Xiaofan; Chen, Shuang; Ye, Ning; Tian, Yichen; Zhang, Lijun

2018-04-10

Despite current interest in the unfavorable impact of cardiometabolic index (CMI) and lipid accumulation product (LAP) on diabetes and cardiovascular risk, information regarding the relation of CMI and LAP to left ventricular (LV) geometry has not been specifically addressed. We aimed to examine the hypothesis: (1) CMI and LAP represent an independent determinant of LV remodeling in general population of rural China; (2) there are gender differences in obesity-related alterations in terms of LV morphology. The sample for this cross-sectional analysis included 11,258 participants (mean age 53.9 years; 54.0% females) who underwent assessment of basic metabolic and anthropometric parameters in rural areas of northeast China. Comprehensive echocardiography-defined LV geometric pattern was determined according to left ventricular mass index and relative wall thickness. The prevalence rate of eccentric and concentric LV hypertrophy (LVH) presented a proportional increase with elevated quartiles of CMI and LAP in a dose-response manner (all P < 0.005). When CMI and LAP were entered as a continuous variable in multivariable adjusted model, we observed the independent effect of 1 SD increment in CMI and LAP with the probability of eccentric and concentric LVH, while this relationship was more pronounced in females than in males. Likewise, the odds ratio comparing the top versus bottom quartiles of CMI were 2.105 (95%CI:1.600-2.768) for eccentric LVH and 2.236 (95%CI:1.419-3.522) for concentric LVH in females. Males in the highest CMI quartile exhibited a nearly doubled (OR:1.724, 95%CI:1.287-2.311) and 1.523-fold (95%CI:1.003-2.313) greater risk of eccentric and concentric LVH, respectively. Increasing LAP entailed a higher possibility of eccentric LVH by a factor of 3.552 and 1.768 in females and males, respectively. In contrast to females, where LAP fourth quartile and concentric LVH were positively associated (OR:2.544, 95%CI:1.537-4.209), higher LAP did not correlate with concentric LVH in males (OR:1.234, 95%CI:0.824-1.849). CMI and LAP give rise to a new paradigm of accounting for gender difference in obesity-related abnormal LV geometry, an effect that was substantially greater in females. These two indices, acting in concert, may also be advantageous prognostically for refining cardiovascular risk stratification in individuals with LV remodeling.

Experimental data on single-bolt joints in quasi isotropic graphite/polyimide laminates

NASA Technical Reports Server (NTRS)

Wichorek, G. R.

1982-01-01

Sixteen ply, quasi-isotropic laminates of Celanese Celion 6000/PMR-15 and Celion 6000/LARC-160 with a fiber orientation of (0/45/90/-45) sub 2S were evaluated. Tensile and open hole specimens were tested at room temperature to establish laminate tensile strength and net tensile strength at an unloaded bolt hole. Double lap joint specimens with a single 4.83-mm (0.19 in.) diameter bolt torqued to 1.7 N-m (15 lbf-in.) were tested in tension at temperatures of 116 K (-250F), 297 K (75F), and 589 K (600F). The joint ratios of w/d (specimen width to hole diameter) and e/d (edge distance to hole diameter) were varied from 4 to 6 and from 2 to 4, respectively. The effect of joint geometry and temperature on failure mode and joint stresses are shown. Joint stresses calculated at maximum load for each joint geometry and test temperature are reported. Joint strength in net tension, bearing, and shear out at 116 K (-250F), 297 K (75F), and 589 K (600F) are given for the Celion 6000/PMR-15 and Celion 6000/LARC-160 laminates.

Synthesis and Characterization of Modified Phenylethynyl Terminated Polyimides

NASA Technical Reports Server (NTRS)

Chang, Alice C.

1998-01-01

As an ongoing effort to develop structural adhesives for high performance aerospace applications, recent work has focused on phenylethynyl terminated imide (PETI) oligomers. The work reported herein involves the synthesis and characterization of a series of phenylethynyl containing oligomers designated LARC (TM) MPEI (Modified Phenylethynyl Terminated Polyimide). These oligomers contain mixtures of linear, branched and star-shaped molecules. The fully imidized polymers exhibited minimum melt viscosity as low as 600 poise at 335 C. Ti/Ti lap shear specimens processed at 288 C under 15 psi showed tensile shear strength of approx. 6000 psi and 5200 psi at ambient and 350 F temperatures, respectively. The chemistry and properties of these new MPEIs are presented and compared to an optimized linear PETI, LaRC(Tm) -PETI-5.

Modeling of the fracture behavior of spot welds using advanced micro-mechanical damage models

NASA Astrophysics Data System (ADS)

Sommer, Silke

2010-06-01

This paper presents the modeling of deformation and fracture behavior of resistance spot welded joints in DP600 steel sheets. Spot welding is still the most commonly used joining technique in automotive engineering. In overloading situations like crash joints are often the weakest link in a structure. For those reasons, crash simulations need reliable and applicable tools to predict the load bearing capacity of spot welded components. Two series of component tests with different spot weld diameters have shown that the diameter of the weld nugget is the main influencing factor affecting fracture mode (interfacial or pull-out fracture), load bearing capacity and energy absorption. In order to find a correlation between nugget diameter, load bearing capacity and fracture mode, the spot welds are simulated with detailed finite element models containing base metal, heat affected zone and weld metal in lap-shear loading conditions. The change in fracture mode from interfacial to pull-out or peel-out fracture with growing nugget diameter under lap-shear loading was successfully modeled using the Gologanu-Leblond model in combination with the fracture criteria of Thomason and Embury. A small nugget diameter is identified to be the main cause for interfacial fracture. In good agreement with experimental observations, the calculated pull-out fracture initiates in the base metal at the boundary to the heat affected zone.

Effect of light aging on silicone-resin bond strength in maxillofacial prostheses.

PubMed

Polyzois, Gregory; Pantopoulos, Antonis; Papadopoulos, Triantafillos; Hatamleh, Muhanad

2015-04-01

The aim of this study was to investigate the effect of accelerated light aging on bond strength of a silicone elastomer to three types of denture resin. A total of 60 single lap joint specimens were fabricated with auto-, heat-, and photopolymerized (n = 20) resins. An addition-type silicone elastomer (Episil-E) was bonded to resins treated with the same primer (A330-G). Thirty specimens served as controls and were tested after 24 hours, and the remaining were aged under accelerated exposure to daylight for 546 hours (irradiance 765 W/m(2) ). Lap shear joint tests were performed to evaluate bond strength at 50 mm/min crosshead speed. Two-way ANOVA and Tukey's test were carried out to detect statistical significance (p < 0.05). ANOVA showed that the main effect of light aging was the most important factor determining the shear bond strength. The mean bond strength values ranged from 0.096 to 0.136 MPa. The highest values were recorded for auto- (0.131 MPa) and photopolymerized (0.136 MPa) resins after aging. Accelerated light aging for 546 hours affects the bond strength of an addition-type silicone elastomer to three different denture resins. The bond strength significantly increased after aging for photo- and autopolymerized resins. All the bonds failed adhesively. © 2014 by the American College of Prosthodontists.

Evaluation of the fuselage lap joint fatigue and terminating action repair

NASA Technical Reports Server (NTRS)

Samavedam, Gopal; Thomson, Douglas; Jeong, David Y.

1994-01-01

Terminating action is a remedial repair which entails the replacement of shear head countersunk rivets with universal head rivets which have a larger shank diameter. The procedure was developed to eliminate the risk of widespread fatigue damage (WFD) in the upper rivet row of a fuselage lap joint. A test and evaluation program has been conducted by Foster-Miller, Inc. (FMI) to evaluate the terminating action repair of the upper rivet row of a commercial aircraft fuselage lap splice. Two full scale fatigue tests were conducted on fuselage panels using the growth of fatigue cracks in the lap joint. The second test was performed to evaluate the effectiveness of the terminating action repair. In both tests, cyclic pressurization loading was applied to the panels while crack propagation was recorded at all rivet locations at regular intervals to generate detailed data on conditions of fatigue crack initiation, ligament link-up, and fuselage fracture. This program demonstrated that the terminating action repair substantially increases the fatigue life of a fuselage panel structure and effectively eliminates the occurrence of cracking in the upper rivet row of the lap joint. While high cycle crack growth was recorded in the middle rivet row during the second test, failure was not imminent when the test was terminated after cycling to well beyond the service life. The program also demonstrated that the initiation, propagation, and linkup of WFD in full-scale fuselage structures can be simulated and quantitatively studied in the laboratory. This paper presents an overview of the testing program and provides a detailed discussion of the data analysis and results. Crack distribution and propagation rates and directions as well as frequency of cracking are presented for both tests. The progression of damage to linkup of adjacent cracks and to eventual overall panel failure is discussed. In addition, an assessment of the effectiveness of the terminating action repair and the occurrence of cracking in the middle rivet row is provided, and conclusions of practical interest are drawn.

Shear sensing in bonded composites with cantilever beam microsensors and dual-plane digital image correlation

NASA Astrophysics Data System (ADS)

Baur, Jeffery W.; Slinker, Keith; Kondash, Corey

2017-04-01

Understanding the shear strain, viscoelastic response, and onset of damage within bonded composites is critical to their design, processing, and reliability. This presentation will discuss the multidisciplinary research conducted which led to the conception, development, and demonstration of two methods for measuring the shear within a bonded joint - dualplane digital image correlation (DIC) and a micro-cantilever shear sensor. The dual plane DIC method was developed to measure the strain field on opposing sides of a transparent single-lap joint in order to spatially quantify the joint shear strain. The sensor consists of a single glass fiber cantilever beam with a radially-grown forest of carbon nanotubes (CNTs) within a capillary pore. When the fiber is deflected, the internal radial CNT array is compressed against an electrode within the pore and the corresponding decrease in electrical resistance is correlated with the external loading. When this small, simple, and low-cost sensor was integrated within a composite bonded joint and cycled in tension, the onset of damage prior to joint failure was observed. In a second sample configuration, both the dual plane DIC and the hair sensor detected viscoplastic changes in the strain of the sample in response to continued loading.

Statistical Investigation of the Effect of Process Parameters on the Shear Strength of Metal Adhesive Joints

NASA Astrophysics Data System (ADS)

Rajkumar, Goribidanur Rangappa; Krishna, Munishamaih; Narasimhamurthy, Hebbale Narayanrao; Keshavamurthy, Yalanabhalli Channegowda

2017-06-01

The objective of the work was to optimize sheet metal joining parameters such as adhesive material, adhesive thickness, adhesive overlap length and surface roughness for single lap joint of aluminium sheet shear strength using robust design. An orthogonal array, main effect plot, signal-to-noise ratio and analysis of variance were employed to investigate the shear strength of the joints. The statistical result shows vinyl ester is best candidate among other two polymers viz. epoxy and polyester due to its low viscosity value compared to other two polymers. The experiment results shows that the adhesive thickness 0.6 mm, overlap length 50 mm and surface roughness 2.12 µm for obtained maximum shear strength of Al sheet joints. The ANOVA result shows one of the most significant factors is overlap length which affect joint strength in addition to adhesive thickness, adhesive material, and surface roughness. A confirmation test was carried out as the optimal combination of parameters will not match with the any of the experiments in the orthogonal array.

Effect of Post-Braze Heat Treatment on the Microstructure and Shear Strength of Cemented Carbide and Steel Using Ag-Based Alloy

NASA Astrophysics Data System (ADS)

Winardi, Y.; Triyono; Muhayat, N.

2018-03-01

The aim of the present study was to investigate the effect temperature of heat treatment process on the interfacial microstructure and mechanical properties of cemented carbide/carbon steel single lap joint brazed using Ag based alloy filler metal. The brazing process was carried out using torch brazing. Heat treatment process was carried out in induction furnace on the temperature of 700, 725, and 750°C, for 30 minutes. Microstructural examinations and phase analysis were performed using scanning electron microscopy (SEM) equipped with energy dispersion spectrometry (EDS). Shear strength of the joints was measured by the universal testing machine. The results of the microstructural analyses of the brazed area indicate that the increase temperature of treatment lead to the increase of solid solution phase of enrichted Cu. Based on EDS test, the carbon elements spread to all brazed area, which is disseminated by base metals. Shear strength joint is increased with temperature treatment. The highest shear strength of the brazed joint was 214,14 MPa when the heated up at 725°C.

A critical study of the role of the surface oxide layer in titanium bonding

NASA Technical Reports Server (NTRS)

Dias, S.; Wightman, J. P.

1982-01-01

The molecular understanding of the role which the surface oxide layer of the adherend plays in titanium bonding is studied. The effects of Ti6-4 adherends pretreatment, bonding conditions, and thermal aging of the lap shear specimens were studied. The use of the SEM/EDAX and ESCA techniques to study surface morphology and surface composition was emphasized. In addition, contact angles and both infrared and visible reflection spectroscopy were used in ancillary studies.

Effect of formation and state of interface on joint strength in friction stir spot welding for advanced high strength steel sheets

NASA Astrophysics Data System (ADS)

Taniguchi, Koichi; Matsushita, Muneo; Ikeda, Rinsei; Oi, Kenji

2014-08-01

The tensile shear strength and cross tension strength of friction stir spot welded joints were evaluated in the cases of lap joints of 270 N/mm2 grade and 980 N/mm2 grade cold rolled steel sheets with respect to the stir zone area, hardness distribution, and interface condition between the sheets. The results suggested that both the tensile shear strength and cross tension strength were based on the stir zone area and its hardness in both grades of steel. The "hook" shape of the interface also affected the joint strength. However, the joining that occurred across the interfaces had a significant influence on the value of the joint strength in the case of the 270 N/mm2 grade steel.

The activity of the artemisinic aldehyde Δ11(13) reductase promoter is important for artemisinin yield in different chemotypes of Artemisia annua L.

PubMed

Yang, Ke; Monfared, Sajad Rashidi; Monafared, Rashidi Sajad; Wang, Hongzhen; Lundgren, Anneli; Brodelius, Peter E

2015-07-01

The artemisinic aldehyde double bond reductase (DBR2) plays an important role in the biosynthesis of the antimalarial artemisinin in Artemisia annua. Artemisinic aldehyde is reduced into dihydroartemisinic aldehyde by DBR2. Artemisinic aldehyde can also be oxidized by amorpha-4,11-diene 12-hydroxylase and/or aldehyde dehydrogenase 1 to artemisinic acid, a precursor of arteannuin B. In order to better understand the effects of DBR2 expression on the flow of artemisinic aldehyde into either artemisinin or arteannuin B, we determined the content of dihydroartemisinic aldehyde, artemisinin, artemisinic acid and arteannuin B content of A. annua varieties sorted into two chemotypes. The high artemisinin producers (HAPs), which includes the '2/39', 'Chongqing' and 'Anamed' varieties, produce more artemisinin than arteannuin B; the low artemisinin producers (LAPs), which include the 'Meise', 'Iran#8', 'Iran#14', 'Iran#24' and 'Iran#47' varieties, produce more arteannuin B than artemisinin. Quantitative PCR showed that the relative expression of DBR2 was significantly higher in the HAP varieties. We cloned and sequenced the promoter of the DBR2 gene from varieties of both the LAP and the HAP groups. There were deletions/insertions in the region just upstream of the ATG start codon in the LAP varities, which might be the reason for the different promoter activities of the HAP and LAP varieties. The relevance of promoter variation, DBR2 expression levels and artemisinin biosynthesis capabilities are discussed and a selection method for HAP varieties with a DNA marker is suggested. Furthermore, putative cis-acting regulatory elements differ between the HAP and LAP varieties.

Experimental study on the strength of double shear timber connection using bamboo dowel fastener

NASA Astrophysics Data System (ADS)

Anshari, Buan; Sugiartha, Wayan; Mahmud, Fathmah; Rofaida, Aryani; Pathurrahman

2017-11-01

Utilization of timber and bamboo as building materials was very promising for now and future. As renewable resources they have high mechanical properties, lightweight, environmentally friendly and economic. Utilization of bamboo as connector was rarely published. Therefore, this study focused on utilization of non-metal material as connector in timber structure especially for beam and column. This research was conducted in the laboratory to examine the strength of double shear timber connection by using glued in rods (bamboo dowel) as connector with variation of adhesive thickness. As control specimen was used bamboo dowel Ø14 mm without adhesive in double shear connection. The results showed that the strength of double shear timber connection by using glued in rods (bamboo dowel) as connector could increased by 41% to resist axial force higher than the control one.

Effect of Interfacial Reaction on the Mechanical Performance of Steel to Aluminum Dissimilar Ultrasonic Spot Welds

NASA Astrophysics Data System (ADS)

Xu, Lei; Wang, Li; Chen, Ying-Chun; Robson, Joe D.; Prangnell, Philip B.

2016-01-01

The early stages of formation of intermetallic compounds (IMC) have been investigated in dissimilar aluminum to steel welds, manufactured by high power (2.5 kW) ultrasonic spot welding (USW). To better understand the influence of alloy composition, welds were produced between a low-carbon steel (DC04) and two different aluminum alloys (6111 and 7055). The joint strengths were measured in lap shear tests and the formation and growth behavior of IMCs at the weld interface were characterized by electron microscopy, for welding times from 0.2 to 2.4 seconds. With the material combinations studied, the η (Fe2Al5) intermetallic phase was found to form first, very rapidly in the initial stage of welding, with a discontinuous island morphology. Continuous layers of η and then θ (FeAl3) phase were subsequently seen to develop on extending the welding time to greater than 0.7 second. The IMC layer formed in the DC04-AA7055 combination grew thicker than for the DC04-AA6111 welds, despite both weld sets having near identical thermal histories. Zinc was also found to be dissolved in the IMC phases when welding with the AA7055 alloy. After post-weld aging of the aluminum alloy, fracture in the lap shear tests always occurred along the joint interface; however, the DC04-AA6111 welds had higher fracture energy than the DC04-AA7055 combination.

Laser Ablation Surface Preparation of Ti-6A1-4V for Adhesive Bonding

NASA Technical Reports Server (NTRS)

Palmieri, Frank L.; Watson, Kent A.; Morales, Guillermo; Williams, Thomas; Hicks, Robert; Wohl, Christopher J.; Hopkins, John W.; Connell, John W.

2012-01-01

Adhesive bonding offers many advantages over mechanical fastening, but requires certification before it can be incorporated in primary structures for commercial aviation without disbond-arrestment features or redundant load paths. Surface preparation is widely recognized as the key step to producing robust and predictable bonds. Laser ablation imparts both topographical and chemical changes to a surface which can lead to increased bond durability. A laser based process provides an alternative to chemical-dip, manual abrasion and grit blast treatments which are expensive, hazardous, polluting, and less precise. This report documents preliminary testing of a surface preparation technique using laser ablation as a replacement for the chemical etch and abrasive processes currently applied to Ti-6Al-4V alloy adherends. Failure mode, surface roughness, and chemical makeup were analyzed using fluorescence enhanced visualization, microscopy, and X-ray photoelectron spectroscopy, respectively. Single lap shear tests were conducted on bonded and aged specimens to observe bond strength retention and failure mode. Some promising results showed increasing strength and durability of lap shear specimens as laser ablation coverage area and beam intensity increased. Chemical analyses showed trends for surface chemical species which correlated with improved bond strength and durability. Combined, these results suggest that laser ablation is a viable process for inclusion with or/and replacement of one or more currently used titanium surface treatments. On-going work will focus on additional mechanical tests to further demonstrate improved bond durability.

Conductive films of silver nanoparticles as novel susceptors for induction welding of thermoplastic composites

NASA Astrophysics Data System (ADS)

Dermanaki Farahani, Rouhollah; Janier, Mathieu; Dubé, Martine

2018-03-01

In the present work, a conductive film of silver nanoparticles (nAg) as a novel heating element type, called susceptor, was developed and tested for induction welding of carbon fiber/polyphenylene sulfide (CF/PPS) thermoplastic composites, i.e., unidirectional pre-impregnated 16 plies of CF/PPS compression-molded in a quasi-isotropic stacking sequence. The nAg were synthesized, dispersed in deionized (DI) water and casted onto a pure PPS film, resulting in a conductive film upon the evaporation of DI water and thermal post-annealing. The thermal annealing at 250 °C significantly (by 7 orders) decreased the film’s electrical resistivity from 9.4 × 103 down to 3.1 × 10-4 Ω cm. The new susceptors led to fast heating rates in induction welding when compared to the standard stainless steel mesh susceptors under similar welding conditions. Lap shear mechanical testing revealed that the apparent lap shear strength (LSS) is sensitive to the susceptors’ resistivity and the input current. A relatively high LSS value was achieved for the specimens welded using the new susceptors which exceeded the value of those welded using stainless steel mesh susceptors (28.3 MPa compared to 20 MPa). The weld interface and specimens’ cross-section observation revealed that the nAg were dispersed and embedded into the resin upon welding. This study contains preliminary results that show high potential of nanoparticles as effective susceptors to further improve the mechanical performance of the joints in welding of thermoplastic composites.

Joining of polypropylene/polypropylene and glass fiber reinforced polypropylene composites

NASA Astrophysics Data System (ADS)

Zhang, Jianguang

Joining behavior of polypropylene (PP) to PP and long glass fiber reinforced polypropylene (LFT) to LFT were investigated. Adhesive bonding was used to join PP/PP. Both adhesive bonding and ultrasonic welding were used to join LFT/LFT. Single-lap shear testing and low velocity impact (LVI) testing were used to evaluate the performance of bonded structures. The two-part acrylic adhesive DP8005 was determined to be the best among the three adhesive candidates, which was attributed to its low surface energy. The impact resistance of LFT/LFT joints, normalized with respect to thickness, was higher than that of PP/PP joints because of higher stiffness of LFT/LFT joints. The stress states in the adhesive layer of adhesively bonded structures were analyzed using ANSYS and LS-DYNA to simulate the single-lap shear testing and LVI testing, respectively. The shear and peel stresses peaked at the edges of the adhesive layer. Compared to LFT/LFT joints, higher peel stress occurred in the adhesive layer in the PP/PP joints in tension. Impact response of adhesively bonded structures as evaluated by LS-DYNA showed good agreement with the experimental results. The effect of weld time and weld pressure on the shear strength of ultrasonically welded LFT/LFT was evaluated. With higher weld pressure, less time was required to obtain a complete weld. At longer weld times, lower weld pressure was required. From the 15 weld conditions studied, a weld map was obtained that provides conditions to achieve a complete weld. Nanoindentation was used to evaluate the effect of ultrasonic weld on the modulus and hardness of the PP matrix. Modulus and hardness of the PP matrix were slightly decreased by ultrasonic welding possibly due to the decrease in the molecular weight. The temperature profile in LFT/LFT in the transverse direction during ultrasonic welding was analyzed by two ANSYS-based thermal models: (a) one in which heat generated by interfacial friction was treated as a heat flux and (b) one in which heat was generated in a thin slab at the interface. The weld map obtained from the thin slab model was closer to the one obtained experimentally.

Characterization of the porosity of human dental enamel and shear bond strength in vitro after variable etch times: initial findings using the BET method.

PubMed

Nguyen, Trang T; Miller, Arthur; Orellana, Maria F

2011-07-01

(1) To quantitatively characterize human enamel porosity and surface area in vitro before and after etching for variable etching times; and (2) to evaluate shear bond strength after variable etching times. Specifically, our goal was to identify the presence of any correlation between enamel porosity and shear bond strength. Pore surface area, pore volume, and pore size of enamel from extracted human teeth were analyzed by Brunauer-Emmett-Teller (BET) gas adsorption before and after etching for 15, 30, and 60 seconds with 37% phosphoric acid. Orthodontic brackets were bonded with Transbond to the samples with variable etch times and were subsequently applied to a single-plane lap shear testing system. Pore volume and surface area increased after etching for 15 and 30 seconds. At 60 seconds, this increase was less pronounced. On the contrary, pore size appears to decrease after etching. No correlation was found between variable etching times and shear strength. Samples etched for 15, 30, and 60 seconds all demonstrated clinically viable shear strength values. The BET adsorption method could be a valuable tool in enhancing our understanding of enamel characteristics. Our findings indicate that distinct quantitative changes in enamel pore architecture are evident after etching. Further testing with a larger sample size would have to be carried out for more definitive conclusions to be made.

The Shear Strength and Fracture Behavior of Sn-Ag- xSb Solder Joints with Au/Ni-P/Cu UBM

NASA Astrophysics Data System (ADS)

Lee, Hwa-Teng; Hu, Shuen-Yuan; Hong, Ting-Fu; Chen, Yin-Fa

2008-06-01

This study investigates the effects of Sb addition on the shear strength and fracture behavior of Sn-Ag-based solders with Au/Ni-P/Cu underbump metallization (UBM) substrates. Sn-3Ag- xSb ternary alloy solder joints were prepared by adding 0 wt.% to 10 wt.% Sb to a Sn-3.5Ag alloy and joining them with Au/Ni-P/Cu UBM substrates. The solder joints were isothermally stored at 150°C for up to 625 h to study their microstructure and interfacial reaction with the UBM. Single-lap shear tests were conducted to evaluate the mechanical properties, thermal resistance, and failure behavior. The results show that UBM effectively suppressed intermetallic compound (IMC) formation and growth during isothermal storage. The Sb addition helped to refine the Ag3Sn compounds, further improving the shear strength and thermal resistance of the solders. The fracture behavior evolved from solder mode toward the mixed mode and finally to the IMC mode with increasing added Sb and isothermal storage time. However, SnSb compounds were found in the solder with 10 wt.% Sb; they may cause mechanical degradation of the solder after long-term isothermal storage.

Improved metal-adhesive polymers from copper(I)-catalyzed azide-alkyne cycloaddition.

PubMed

Accurso, Adrian A; Delaney, Mac; O'Brien, Jeff; Kim, Hyonny; Iovine, Peter M; Díaz Díaz, David; Finn, M G

2014-08-18

Electrically conductive adhesive polymers offer many potential advantages relative to Sn-Pb solders, including reduced toxicity, low cost, low processing temperatures, and the ability to make application-specific formulations. Polymers generated from the copper(I)-catalyzed cycloaddition (CuAAC) reaction between multivalent azides and alkynes have previously been identified as strong metal-binding adhesives. Herein we demonstrate that the performance of these materials can be remarkably improved by the incorporation of a flexibility-inducing difunctionalized component and a tertiary amine additive in optimized concentrations. The best formulations were identified by means of rapid adhesion testing of a library of potential candidates by using a custom-built instrument and validated in an American Society for Testing and Materials (ASTM)-standard lap-shear test. Characteristic phase transitions were identified by differential scanning calorimetry (DSC) for adhesives with and without the additives as a function of curing temperature. The incorporation of flexible components was found to more than double the strength of the adhesive. Moreover, the adhesive was made electrically conductive by the inclusion of 20 wt% silver-coated copper flakes and further improved in this regard by the incorporation of multiwalled carbon nanotubes in the formulation. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

The Effect of Welding Energy on the Microstructural and Mechanical Properties of Ultrasonic-Welded Copper Joints

PubMed Central

Yang, Jingwei; Cao, Biao; Lu, Qinghua

2017-01-01

The effects of welding energy on the mechanical and microstructural characteristics of ultrasonic-welded pure copper plates were investigated. Complex dynamic recrystallization and grain growth occurred inside the weld zone during ultrasonic welding. At a low welding energy, a thin band of straight weld interfaces was observed and had an ultra-fine grain structure. With an increase in welding energy, the weld interface progressively changed from flat to sinusoidal, and eventually turned into a convoluted wavy pattern, bearing similarities to shear instabilities, as observed in fluid dynamics. The lap shear load of the joints initially increased and then remained stable as the welding energy increased. The tensile characteristics of the joints significantly depended on the development of plastic deformation at the interface. The influence of the microstructure on the hardness was also discussed. PMID:28772553

Polymer matrix composites on LDEF experiments M0003-9 and M0003-10

NASA Technical Reports Server (NTRS)

Steckel, Gary L.; Cookson, Thomas; Blair, Christopher

1992-01-01

Over 250 polymer matrix composites were exposed to the natural space environment on Long Duration Exposure Facility (LDEF) experiments M0003-9 and 10. The experiments included a wide variety of epoxy, thermoplastic, polyimide, and bismalimide matrix composites reinforced with graphite, glass, or organic fibers. A review of the significant observations and test results obtained to date is presented. Estimated recession depths from atomic oxygen exposure are reported and the resulting surface morphologies are discussed. The effects of the LDEF exposure on the flexural strength and modulus, short beam shear strength, and coefficient of thermal expansion of several classes of bare and coated composites are reviewed. Lap shear data are presented for composite-to-composite and composite-to-aluminum alloy samples that were prepared using different bonding techniques and subsequently flown on LDEF.

The Effect of Welding Energy on the Microstructural and Mechanical Properties of Ultrasonic-Welded Copper Joints.

PubMed

Yang, Jingwei; Cao, Biao; Lu, Qinghua

2017-02-16

The effects of welding energy on the mechanical and microstructural characteristics of ultrasonic-welded pure copper plates were investigated. Complex dynamic recrystallization and grain growth occurred inside the weld zone during ultrasonic welding. At a low welding energy, a thin band of straight weld interfaces was observed and had an ultra-fine grain structure. With an increase in welding energy, the weld interface progressively changed from flat to sinusoidal, and eventually turned into a convoluted wavy pattern, bearing similarities to shear instabilities, as observed in fluid dynamics. The lap shear load of the joints initially increased and then remained stable as the welding energy increased. The tensile characteristics of the joints significantly depended on the development of plastic deformation at the interface. The influence of the microstructure on the hardness was also discussed.

Materials 88: Materials and Engineering Design Held in London, England, on 9-13 May 1988

DTIC Science & Technology

1988-01-01

process changing from a temperatures in the rings would have favoured the polishing mechanism for the lapped specimens, to microplastic abrasion and...UE - ultimate shear strength of epoxy adhesive Solid line EE - elastic limit of epoxy adhesive. Sea . UE E4QGE E 7.3S 27.5- E 5 1. . 5 1.- Ps. 7. 7 P zs...is well predicted. The accuracy of the predicted contribute negatively to kA due to the presence of the abpoitc B.E. and initial microplasticity

Weld bonding of titanium with polyimide adhesives

NASA Technical Reports Server (NTRS)

Vaughan, R. W.; Sheppard, C. H.; Orell, M. K.

1975-01-01

A conductive adhesive primer and a capillary flow adhesive were developed for weld bonding titanium alloy joints. Both formulations contained ingredients considered to be non-carcinogenic. Lap-shear joint test specimens and stringer-stiffened panels were weld bonded using a capillary flow process to apply the adhesive. Static property information was generated for weld bonded joints over the temperature range of 219K (-65 F) to 561K (550 F). The capillary flow process was demonstrated to produce weld bonded joints of equal strength to the weld through weld bonding process developed previously.

Probabilistic and Possibilistic Analyses of the Strength of a Bonded Joint

NASA Technical Reports Server (NTRS)

Stroud, W. Jefferson; Krishnamurthy, T.; Smith, Steven A.

2001-01-01

The effects of uncertainties on the strength of a single lap shear joint are explained. Probabilistic and possibilistic methods are used to account for uncertainties. Linear and geometrically nonlinear finite element analyses are used in the studies. To evaluate the strength of the joint, fracture in the adhesive and material strength failure in the strap are considered. The study shows that linear analyses yield conservative predictions for failure loads. The possibilistic approach for treating uncertainties appears to be viable for preliminary design, but with several qualifications.

Material properties of novel polymeric films

NASA Astrophysics Data System (ADS)

Kim, Gene

This dissertation will study the material properties of two types of novel polymer films (polyelectrolyte multilayer films and photolithographic polymer films). The formation of polylelectrolyte multilayer films onto functionalized aluminum oxide surfaces and functionalized poly(ethylene terephthaltate) (PET) were studied. Functionalization of the aluminum oxide surfaces was achieved via silane coupling. Functionalization of PET surfaces was achieved via hydrolysis and amidation. Surface characterization techniques such as X-ray photoelectron spectroscopy (XPS) and dynamic contact angle measurements were used to monitor the polyelectrolyte multilayer formation. Mechanical properties of the aluminum oxide supported polyelectrolyte multilayer films were tested using a simplified peel test. XPS was used to analyze the surfaces before and after peel. Single lap shear joint specimens were constructed to test the adhesive shear strength of the PET-supported polyelectrolyte multilayer film samples with the aid of a cyanoacrylate adhesive. The adhesive shear strength and its relation with the type of functionalization, number of polyelectrolyte layers, and the effect of polyelectrolyte conformation using added salt were explored. Also, characterization on the single lap joints after adhesive failure was carried out to determine the locus of failure within the multilayers by using XPS and SEM. Two types of photolithographic polymers were formulated and tested. These two polymers (photocrosslinkable polyacrylate (PUA), and a photocrosslinkable polyimide (HRP)) were used to investigate factors that would affect the structural integrity of these particular polymers under environmental variables such as processing (time, UV cure, pressure, and temperature) and ink exposure. Thermomechanical characterization was carried out to see the behavior of these two polymers under these environmental variables. Microscopic techniques were employed to study the morphological behavior of the two polymer systems. Also, unique in-house characterization methods such as the vibrational holographic interferometry to measure residual stress in these polymer coatings upon processing, and the environmental tensile tester (ETT) to measure ink diffusion and swelling stresses were used to further characterize these two polymers.

Conductive films of silver nanoparticles as novel susceptors for induction welding of thermoplastic composites.

PubMed

Farahani, Rouhollah Dermanaki; Janier, Mathieu; Dubé, Martine

2018-03-23

In the present work, a conductive film of silver nanoparticles (nAg) as a novel heating element type, called susceptor, was developed and tested for induction welding of carbon fiber/polyphenylene sulfide (CF/PPS) thermoplastic composites, i.e., unidirectional pre-impregnated 16 plies of CF/PPS compression-molded in a quasi-isotropic stacking sequence. The nAg were synthesized, dispersed in deionized (DI) water and casted onto a pure PPS film, resulting in a conductive film upon the evaporation of DI water and thermal post-annealing. The thermal annealing at 250 °C significantly (by 7 orders) decreased the film's electrical resistivity from 9.4 × 10 3 down to 3.1 × 10 -4 Ω cm. The new susceptors led to fast heating rates in induction welding when compared to the standard stainless steel mesh susceptors under similar welding conditions. Lap shear mechanical testing revealed that the apparent lap shear strength (LSS) is sensitive to the susceptors' resistivity and the input current. A relatively high LSS value was achieved for the specimens welded using the new susceptors which exceeded the value of those welded using stainless steel mesh susceptors (28.3 MPa compared to 20 MPa). The weld interface and specimens' cross-section observation revealed that the nAg were dispersed and embedded into the resin upon welding. This study contains preliminary results that show high potential of nanoparticles as effective susceptors to further improve the mechanical performance of the joints in welding of thermoplastic composites.

SDU6 Interior Liner Testing & Evaluation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Skidmore, T. E.

Two liner materials (Marseal® M-3500 and REMA Chemoline® 4CN) proposed for use as a liner inside the Saltstone Disposal Unit 6 (SDU6) were subjected to specific ASTM tests (tensile and lap-shear) after immersion in 50% and 100% simulant solutions for 1000 hours at the Savannah River Ecology Laboratory. Both liner materials exhibited good resistance to the simulant chemistry, at least based on the tests performed and the test duration/conditions imposed. In lap-shear tests, both materials failed in the base material rather than peeling apart, confirming good adhesion. The REMA 4CN bromobutyl elastomer showed superior bonding characteristics and absence of warpingmore » or delamination at the conditions tested. The Marseal M-3500 material (PVC/EVA blend with polyester reinforcement) exhibited deformation and debonding in some locations. The cause of the deformation and delamination observed in the Marseal M-3500 material is not fully known, but possibly attributed to thermomechanical stress at immersion temperatures, and the thermoplastic nature of the material. The immersion temperature (68 °C) is slightly greater than the maximum use temperature limit quoted for the Marseal M- 3500 liner (65 °C), though the basis for the service limit is unknown. The testing performed was limited in scope and only for these two liner materials. These tests were primarily performed to screen for severe incompatibility or short-term degradation in Saltstone bleedwater simulants at bounding solution temperatures. Additional testing is recommended to assess long-term performance and the overall service life of the liner.« less

Plant Leucine Aminopeptidases Moonlight as Molecular Chaperones to Alleviate Stress-induced Damage*

PubMed Central

Scranton, Melissa A.; Yee, Ashley; Park, Sang-Youl; Walling, Linda L.

2012-01-01

Leucine aminopeptidases (LAPs) are present in animals, plants, and microbes. In plants, there are two classes of LAPs. The neutral LAPs (LAP-N and its orthologs) are constitutively expressed and detected in all plants, whereas the stress-induced acidic LAPs (LAP-A) are expressed only in a subset of the Solanaceae. LAPs have a role in insect defense and act as a regulator of the late branch of wound signaling in Solanum lycopersicum (tomato). Although the mechanism of LAP-A action is unknown, it has been presumed that LAP peptidase activity is essential for regulating wound signaling. Here we show that plant LAPs are bifunctional. Using three assays to monitor protein protection from heat-induced damage, it was shown that the tomato LAP-A and LAP-N and the Arabidopsis thaliana LAP1 and LAP2 are molecular chaperones. Assays using LAP-A catalytic site mutants demonstrated that LAP-A chaperone activity was independent of its peptidase activity. Furthermore, disruption of the LAP-A hexameric structure increased chaperone activity. Together, these data identify a new class of molecular chaperones and a new function for the plant LAPs as well as suggesting new mechanisms for LAP action in the defense of solanaceous plants against stress. PMID:22493451

Comparison of the Effects of Debonds and Voids in Adhesive Joints

NASA Technical Reports Server (NTRS)

Rossettos, J. N.; Lin, P.; Nayeb-Hashemi, Hamid

1997-01-01

An analytical model is developed to compare the effects of voids an debonds on the interfacial shear stresses between the adherends and the adhesive in simple lap joints. Since the adhesive material above the debond may undergo some extension (either due to applied load or thermal expansion or both), a modified shear lag model, where the adhesive can take an extensional as well as shear deformation, is used in the analysis. The adherends take on only axial loads and act as membranes. Two coupled nondimensional differential equations are derived, and in general, five parameters govern the stress distribution in the overlap region. As expected, the major differences between the debond and the void occur for the stresses near the edge of the defect itself. Whether the defect is a debond or a void, is hardly discernible by the stresses at the overlap region. If the defect occurs precisely at or very close to either end of the overlap, however, differences of the order of 20 percent in the peak stresses can be obtained.

Multi-Scale Computational Modeling of Ni-Base Superalloy Brazed Joints for Gas Turbine Applications

NASA Astrophysics Data System (ADS)

Riggs, Bryan

Brazed joints are commonly used in the manufacture and repair of aerospace components including high temperature gas turbine components made of Ni-base superalloys. For such critical applications, it is becoming increasingly important to account for the mechanical strength and reliability of the brazed joint. However, material properties of brazed joints are not readily available and methods for evaluating joint strength such as those listed in AWS C3.2 have inherent challenges compared with testing bulk materials. In addition, joint strength can be strongly influenced by the degree of interaction between the filler metal (FM) and the base metal (BM), the joint design, and presence of flaws or defects. As a result, there is interest in the development of a multi-scale computational model to predict the overall mechanical behavior and fitness-for-service of brazed joints. Therefore, the aim of this investigation was to generate data and methodology to support such a model for Ni-base superalloy brazed joints with conventional Ni-Cr-B based FMs. Based on a review of the technical literature a multi-scale modeling approach was proposed to predict the overall performance of brazed joints by relating mechanical properties to the brazed joint microstructure. This approach incorporates metallurgical characterization, thermodynamic/kinetic simulations, mechanical testing, fracture mechanics and finite element analysis (FEA) modeling to estimate joint properties based on the initial BM/FM composition and brazing process parameters. Experimental work was carried out in each of these areas to validate the multi-scale approach and develop improved techniques for quantifying brazed joint properties. Two Ni-base superalloys often used in gas turbine applications, Inconel 718 and CMSX-4, were selected for study and vacuum furnace brazed using two common FMs, BNi-2 and BNi-9. Metallurgical characterization of these brazed joints showed two primary microstructural regions; a soft, ductile a-Ni phase that formed at the joint interface and a hard, brittle multi-phase centerline eutectic. CrB and Ni3B type borides were identified in the eutectic region via electron probe micro-analysis, and a boron diffusion gradient was observed in the BM adjacent to the joint. The volume fraction of centerline eutectic was found to be highly dependent on the extent of the boron diffusion that occurred during brazing and therefore a function of the primary process parameters; hold time, temperature, FM/BM composition, and joint gap. Thermo-Calc(TM) and DICTRA(TM) simulations were used to model the BM dissolution, isothermal solidification and phase transformations that occurred during brazing to predict the final joint microstructure based on these process parameters. Good agreement was found between experimental and simulated joint microstructures at various joint gaps demonstrating the application of these simulations for brazed joints. However, thermodynamic/kinetic databases available for brazing FMs were limited. A variety of mechanical testing was performed to determine the mechanical properties of CMSX-4/BNi-2 and IN718/BNi-2 brazed joints including small-scale tensile tests, standard-size butt joints and lap shear tests. Small-scale tensile testing provided a novel method for studying microstructure-property relationships in brazed joints and indicated that both joint strength and ductility decrease significantly with an increase in the volume fraction of centerline eutectic. In-situ observations during small-scale testing also showed strain localization and crack initiation occurs around the hard, eutectic phases in the joint microstructure during loading. The average tensile strength for standard-size IN718/BNi-2 butt joints containing a low volume fraction of centerline eutectic was found to be 152.8 ksi approximately 90% of the BM yield strength (˜170 ksi). The average lap shear FM stress was found to decrease from 70 to 20 ksi for IN718/BNi-2 joints and from 50 to 15 ksi for CMSX-4/BNi-2 as the overlap was increased from 1T to 5T due to non-uniform stress/strain distribution across the joint. Digital image correlation techniques and FEA models of the lap shear brazed joints were developed to assess the strain distributions across the overlap. Results were used to validate the use of damage zone models for predicting the load carrying capacity of lap shear brazed joints and suggest that the damage zone is independent of the overlap length. To account for the presence of flaws and defects in fitness-for-service assessments of brazed joints determination of the average fracture toughness (KIC) is necessary. Currently no standard exists to measure the KIC for brazed joints, so three test methods were evaluated in this investigation on IN718/BNi-2 brazed joints. The compact tension and double cantilever beam test methods were found to give the most conservative KIC values of 16.42 and 14.42 ksivin respectively. Linear-elastic FEA models of the test specimens were used to validate the calculated KIC values. Similar to joint strength the fracture toughness appeared to be strongly influenced by the volume fraction of centerline eutectic phases. (Abstract shortened by ProQuest.).

Semi-2-interpenetrating polymer networks of high temperature systems

NASA Technical Reports Server (NTRS)

Hanky, A. O.; St. Clair, T. L.

1985-01-01

A semi-interpenetrating (semi-IPN) polymer system of the semi-2-IPN type is described in which a polymer of acetylene-terminated imidesulfone (ATPISO2) is cross linked in the presence of polyimidesulfone (PISO2). Six different formulations obtained by mixing of either ATPISO2-1n or ATPISO2-3n with PISO2 in three different proportions were characterized in terms of glass transition temperature, thermooxidative stability, inherent viscosity, and dynamic mechanical properties. Adhesive (lap shear) strength was tested at elevated temperatures on aged samples of adhesive scrim cloth prepared from each resin. Woven graphite (Celion 1000)/polyimide composites were tested for flexural strength, flexural modulus, and shear strength. The network polymers have properties intermediate between those of the component polymers alone, have greatly improved processability over either polyimide, and are able to form good adhesive bonds and composites, making the semi-2-IPN systems superior materials for aerospace structures.

Solid state welding processes for an oxide dispersion strengthened nickel-chromium-aluminum alloy

NASA Technical Reports Server (NTRS)

Moore, T. J.

1975-01-01

Solid-state welding processes were evaluated for joining TD-NiCrAl (Ni-16Cr-4Al-2ThO2) alloy sheet. Both hot-press and resistance spot welding techniques were successfully applied in terms of achieving grain growth across the bond line. Less success was achieved with a resistance seam welding process. In stress-rupture shear and tensile shear tests of lap joints at 1100 C, most failures occurred in the parent material, which indicates that the weld quality was good and that the welds were not a plane of weakness. The overall weld quality was not as good as previously attained with TD-NiCr, probably because the presence of alumina at the faying surfaces and the developmental TD-NiCrAl sheet, which was not of the quality of the TD-NiCr sheet in terms of surface flatness and dimensional control.

Modeling of fracture and durability of paste-bonded composite joints subjected to hygro-thermal-mechanical loading

NASA Astrophysics Data System (ADS)

Harris, David Lee

The objective of the research is to characterize the behavior of composite/composite joints with paste adhesive using both experimental testing and analytical modeling. In comparison with the conventional tape adhesive, joining composites using paste adhesive provides several advantages. The carbon fiber laminate material systems employed in this study included IM7 carbon fibers and 977-3 epoxy matrix assembled in prepreg tape, and AS4 carbon fibers and 977-3 epoxy matrix as a five-harness satin weave. The adhesive employed was EA 9394 epoxy. All laminates and test specimens were fabricated and inspected by Boeing using their standard propriety procedures. Three types of test specimens were used in the program. They were bonded double-lap shear (DLS), bonded double cantilever beam (DCB) and bonded interlaminar tension (ILT) specimens. A group of specimens were conditioned at elevated temperature and humidity in an environmental chamber at Boeing's facility and their moisture absorption recorded with time. Specimens were tested at room temperature dry and elevated temperatures. DCB and DLS specimens were tested in fatigue as well as static conditions. Two-dimensional finite element models of the three configurations were developed for determining stresses and strains using the ABAQUS finite element package code. Due to symmetry, only the one-half of the specimen needed to be considered thus reducing computational time. The effect of the test fixture is not taken into account instead equivalent distributed stresses are applied directly on the composite laminates. For each of the specimen, the distribution of Mises stress and the first strain invariant J1 are obtained to identify potential failure locations within a specimen.

Seismic performance of interior precast concrete beam-column connections with T-section steel inserts under cyclic loading

NASA Astrophysics Data System (ADS)

Ketiyot, Rattapon; Hansapinyo, Chayanon

2018-04-01

An experimental investigation was conducted to study the performance of precast beam-column concrete connections using T-section steel inserts into the concrete beam and joint core, under reversed cyclic loading. Six 2/3-scale interior beam-column subassemblies, one monolithic concrete specimen and five precast concrete specimens were tested. One precast specimen was a simple connection for a gravity load resistant design. Other precast specimens were developed with different attributes to improve their seismic performance. The test results showed that the performance of the monolithic specimen M1 represented ductile seismic behavior. Failure of columns and joints could be prevented, and the failure of the frame occurred at the flexural plastic hinge formation at the beam ends, close to the column faces. For the precast specimens, the splitting crack along the longitudinal lapped splice was a major failure. The precast P5 specimen with double steel T-section inserts showed better seismic performance compared to the other precast models. However, the dowel bars connected to the steel inserts were too short to develop a bond. The design of the precast concrete beams with lap splice is needed for longer lap lengths and should be done at the beam mid span or at the low flexural stress region.

Chemical, Calcium Phosphate Cements for Geothermal Wells - Corrosion Protection, Bond Strength and Matrix Self-Healing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sugama, Toshifumi

The data set shows performance of economical calcium phosphate cement (Fondu) blended with fly ash, class F (FAF) in carbon steel corrosion protection tests (corrosion rate, corrosion current and potential), bond- and matrix strength, as well as matrix strength recovery after imposed damage at 300C. The corrosion protection and lap-shear bond strength data are given for different Fondu/FAF ratios, the matrix strength data recoveries are reported for 60/40 weight % Fondu/FAF ratios. Effect of sodium phosphate on bond strength, corrosion protection and self-healing is demonstrated.

Miniaturization of Micro-Solder Bumps and Effect of IMC on Stress Distribution

NASA Astrophysics Data System (ADS)

Choudhury, Soud Farhan; Ladani, Leila

2016-07-01

As the joints become smaller in more advanced packages and devices, intermetallic (IMCs) volume ratio increases, which significantly impacts the overall mechanical behavior of joints. The existence of only a few grains of Sn (Tin) and IMC materials results in anisotropic elastic and plastic behavior which is not detectable using conventional finite element (FE) simulation with average properties for polycrystalline material. In this study, crystal plasticity finite element (CPFE) simulation is used to model the whole joint including copper, Sn solder and Cu6Sn5 IMC material. Experimental lap-shear test results for solder joints from the literature were used to validate the models. A comparative analysis between traditional FE, CPFE and experiments was conducted. The CPFE model was able to correlate the experiments more closely compared to traditional FE analysis because of its ability to capture micro-mechanical anisotropic behavior. Further analysis was conducted to evaluate the effect of IMC thickness on stress distribution in micro-bumps using a systematic numerical experiment with IMC thickness ranging from 0% to 80%. The analysis was conducted on micro-bumps with single crystal Sn and bicrystal Sn. The overall stress distribution and shear deformation changes as the IMC thickness increases. The model with higher IMC thickness shows a stiffer shear response, and provides a higher shear yield strength.

Two-dimensional wavefront reconstruction based on double-shearing and least squares fitting

NASA Astrophysics Data System (ADS)

Liang, Peiying; Ding, Jianping; Zhu, Yangqing; Dong, Qian; Huang, Yuhua; Zhu, Zhen

2017-06-01

The two-dimensional wavefront reconstruction method based on double-shearing and least squares fitting is proposed in this paper. Four one-dimensional phase estimates of the measured wavefront, which correspond to the two shears and the two orthogonal directions, could be calculated from the differential phase, which solves the problem of the missing spectrum, and then by using the least squares method the two-dimensional wavefront reconstruction could be done. The numerical simulations of the proposed algorithm are carried out to verify the feasibility of this method. The influence of noise generated from different shear amount and different intensity on the accuracy of the reconstruction is studied and compared with the results from the algorithm based on single-shearing and least squares fitting. Finally, a two-grating lateral shearing interference experiment is carried out to verify the wavefront reconstruction algorithm based on doubleshearing and least squares fitting.

Evaluation of Surlyn 8920 as PHE Visor Material and Evaluations of New Adhesives for Improving Bonding Between Teflon and Stainless Steel at Cryogenic Temperature

NASA Technical Reports Server (NTRS)

Ray, Asit K.

1991-01-01

Two studies are presented, and in the first study, Surlyn 8920 (an ionic and amorphous low density polyethylene made by Dupont) was evaluated as a possible replacement of Plexyglass G as PHE visor material. Four formulations of the polymer were made by adding different amounts of UV stabilizer, energy quencher, and antioxident in a Brabender Plasticorder. The formulated polymers were molded in the form of sheets in a compression molder. Cut samples from the molded sheets were exposed in a weatherometer and tested on Instron Tensile Tester for strength and elongation. Specially molded samples of the formulated polymers were subjected to Charpy Impact Tests. In the second study, preliminary evaluations of adhesives for improvement of bonding between Teflon and stainless steel (SS) were performed. Kapton, a high temperature polyimide made by Dupont, and a rubber based adhesive made by Potter Paint Co., were evaluated against industrial quality epoxy, the current material used to bond Teflon and SS. The degreased surfaces of the SS discs were etched mechanically, with a few of these etched chemically. The surfaces of the SS discs were etched mechanically, with a few of these etched chemically. Bonding strengths were evaluated using lap shear tests on the Instron Tensile Tester for the samples bonded by Kapton and industrial quality epoxy. Bond strengths were also evaluated using a pull test on the Instron for the samples bonded by Potter adhesive (CWL-152) and industrial quality epoxy. Based on limited lap shear data, Kapton gave bond strength favorable compared to that of industrial epoxy. Based on limited pull test data, Kapton bonded and CWL-152 bonded samples showed poor strength compared to epoxy bonded sample.

C/EBPβ-LAP*/LAP Expression Is Mediated by RSK/eIF4B-Dependent Signalling and Boosted by Increased Protein Stability in Models of Monocytic Differentiation

PubMed Central

Christmann, Martin; Friesenhagen, Judith; Westphal, Andreas; Pietsch, Daniel; Brand, Korbinian

2015-01-01

The transcription factor C/EBPβ plays a key role in monocytic differentiation and inflammation. Its small isoform LIP is associated with proliferation at early premonocytic developmental stages and regulated via mTOR-dependent signalling. During later stages of (pre)monocytic differentiation there is a considerable increase in the large C/EBPβ isoforms LAP*/LAP which inhibit proliferation thus supporting terminal differentiation. Here, we showed in different models of monocytic differentiation that this dramatic increase in the LAP*/LAP protein and LAP/LIP ratio was accompanied by an only modest/retarded mRNA increase suggesting an important role for (post)translational mechanisms. We found that LAP*/LAP formation was induced via MEK/RSK-dependent cascades, whereas mTOR/S6K1 were not involved. Remarkably, LAP*/LAP expression was dependent on phosphorylated eIF4B, an acceleratory protein of RNA helicase eIF4A. PKR inhibition reduced the expression of eIF4B and C/EBPβ in an eIF2α-independent manner. Furthermore, under our conditions a marked stabilisation of LAP*/LAP protein occurred, accompanied by reduced chymotrypsin-like proteasome/calpain activities and increased calpastatin levels. Our study elucidates new signalling pathways inducing LAP*/LAP expression and indicates new alternative PKR functions in monocytes. The switch from mTOR- to RSK-mediated signalling to orchestrate eIF4B-dependent LAP*/LAP translation, accompanied by increased protein stability but only small mRNA changes, may be a prototypical example for the regulation of protein expression during selected processes of differentiation/proliferation. PMID:26646662

Microstructural Characterization of Friction Stir Welded Aluminum-Steel Joints

NASA Astrophysics Data System (ADS)

Patterson, Erin E.; Hovanski, Yuri; Field, David P.

2016-06-01

This work focuses on the microstructural characterization of aluminum to steel friction stir welded joints. Lap weld configuration coupled with scribe technology used for the weld tool have produced joints of adequate quality, despite the significant differences in hardness and melting temperatures of the alloys. Common to friction stir processes, especially those of dissimilar alloys, are microstructural gradients including grain size, crystallographic texture, and precipitation of intermetallic compounds. Because of the significant influence that intermetallic compound formation has on mechanical and ballistic behavior, the characterization of the specific intermetallic phases and the degree to which they are formed in the weld microstructure is critical to predicting weld performance. This study used electron backscatter diffraction, energy dispersive spectroscopy, scanning electron microscopy, and Vickers micro-hardness indentation to explore and characterize the microstructures of lap friction stir welds between an applique 6061-T6 aluminum armor plate alloy and a RHA homogeneous armor plate steel alloy. Macroscopic defects such as micro-cracks were observed in the cross-sectional samples, and binary intermetallic compound layers were found to exist at the aluminum-steel interfaces of the steel particles stirred into the aluminum weld matrix and across the interfaces of the weld joints. Energy dispersive spectroscopy chemical analysis identified the intermetallic layer as monoclinic Al3Fe. Dramatic decreases in grain size in the thermo-mechanically affected zones and weld zones that evidenced grain refinement through plastic deformation and recrystallization. Crystallographic grain orientation and texture were examined using electron backscatter diffraction. Striated regions in the orientations of the aluminum alloy were determined to be the result of the severe deformation induced by the complex weld tool geometry. Many of the textures observed in the weld zone and thermo-mechanically affected zones exhibited shear texture components; however, there were many textures that deviated from ideal simple shear. Factors affecting the microstructure which are characteristic of the friction stir welding process, such as post-recrystallization deformation and complex deformation induced by tool geometry were discussed as causes for deviation from simple shear textures.

TGF-β induces surface LAP expression on murine CD4 T cells independent of Foxp3 induction.

PubMed

Oida, Takatoku; Weiner, Howard L

2010-11-24

It has been reported that human FOXP3(+) CD4 Tregs express GARP-anchored surface latency-associated peptide (LAP) after activation, based on the use of an anti-human LAP mAb. Murine CD4 Foxp3(+) Tregs have also been reported to express surface LAP, but these studies have been hampered by the lack of suitable anti-mouse LAP mAbs. We generated anti-mouse LAP mAbs by immunizing TGF-β(-/-) animals with a mouse Tgfb1-transduced P3U1 cell line. Using these antibodies, we demonstrated that murine Foxp3(+) CD4 Tregs express LAP on their surface. In addition, retroviral transduction of Foxp3 into mouse CD4(+)CD25(-) T cells induced surface LAP expression. We then examined surface LAP expression after treating CD4(+)CD25(-) T cells with TGF-β and found that TGF-β induced surface LAP not only on T cells that became Foxp3(+) but also on T cells that remained Foxp3(-) after TGF-β treatment. GARP expression correlated with the surface LAP expression, suggesting that surface LAP is GARP-anchored also in murine T cells. Unlike human CD4 T cells, surface LAP expression on mouse CD4 T cells is controlled by Foxp3 and TGF-β. Our newly described anti-mouse LAP mAbs will provide a useful tool for the investigation and functional analysis of T cells that express LAP on their surface.

Molecular cloning and characterization of leucine aminopeptidase from Fasciola gigantica.

PubMed

Changklungmoa, Narin; Chaithirayanon, Kulathida; Kueakhai, Pornanan; Meemon, Krai; Riengrojpitak, Suda; Sobhon, Prasert

2012-07-01

M17 leucine aminopeptidase (LAP) is one of a family of metalloexopeptidases, of which short peptide fragments are cleaved from the N-terminals. In this study, the full length of cDNA encoding Fasciola gigantica LAP (FgLAP) was cloned from adult parasites. The amino acid sequences of FgLAP showed a high degree of identity (98%) with that from Fasciola hepatica and a low degree of identities (11% and 9%) with those from cattle and human. Phylogenetic analysis revealed that the FgLAP was closely related and grouped with F. hepatica LAP (FhLAP). Northern analysis showed that FgLAP transcriptional products have 1800 base pairs. Analysis by RNA in situ hybridization indicated that LAP gene was expressed in the cecal epithelial cells of adult parasites. A polyclonal antibody to a recombinant FgLAP (rFgLAP) detected the native LAP protein in various developmental stages of the parasite. In a functional test, this rFgLAP displayed aminolytic activity using a fluorogenic Leu-MCA substrate, and was significantly inhibited by bestatin. Its maximum activity was at pH 8.0 and enhanced by Mn(2+) ions. Localization of LAP proteins by immunohistochemistry and immunofluorescence techniques indicated that the enzyme was distributed in the apical cytoplasm of cecal epithelial cells. Because of its important metabolic role and fairly exposed position, FgLAP is a potential drug target and a possible vaccine candidate against fasciolosis. Copyright © 2012 Elsevier Inc. All rights reserved.

Ultimate Limit State Assessment of Timber Bolt Connection Subjected to Double Unequal Shears

NASA Astrophysics Data System (ADS)

Musilek, Josef; Plachy, Jan

2017-10-01

Nowadays the problems occur when a structure engineer need to assess the ultimate limit state of timber bolt connection which is subjected to double unequal shears. This assessment of ultimate limit state shows the reliability of these connections. In assessing the reliability of this connection in ultimate limit state is a problem, because the formulas and equations that are currently available in design standards and available literature, describing only connections loaded symmetrically - this mean that they describe the timber bolt connection subjected to double equal shears. This fact causes problems because structural engineers have no available support, according to which they could assess reliability of the connection in terms of the ultimate limit state. They must therefore often report following an asymmetrically loaded connections carry about using formulas, which are primarily designed for checking connections loaded symmetrically. This leads logically to the fact that it is not respected by the actual behaviour of the connection in the ultimate limit state. Formulas derived in this paper provide the possibility to assess the ultimate limit state for such connection. The formulas derived in this article allow to carry out a reliability assessment of the ultimate limit state of timber bolt connection subjected to double shear. The using of the formulas derived in this paper leads to better description of the behaviour of this type of connection and also to the more economic design. An example of using these derived formulas is shown. There is shown in this example, how to assess the reliability of timber bolt connection subjected to double unequal shears in terms of ultimate limit states.

Evaluation of Margins of Safety in Brazed Joints

NASA Technical Reports Server (NTRS)

Flom, Yury; Wang, Len; Powell, Mollie M.; Soffa, Matthew A.; Rommel, Monica L.

2009-01-01

One of the essential steps in assuring reliable performance of high cost critical brazed structures is the assessment of the Margin of Safety (MS) of the brazed joints. In many cases the experimental determination of the failure loads by destructive testing of the brazed assembly is not practical and cost prohibitive. In such cases the evaluation of the MS is performed analytically by comparing the maximum design loads with the allowable ones and incorporating various safety or knock down factors imposed by the customer. Unfortunately, an industry standard methodology for the design and analysis of brazed joints has not been developed. This paper provides an example of an approach that was used to analyze an AlBeMet 162 (38%Be-62%Al) structure brazed with the AWS BAlSi-4 (Al-12%Si) filler metal. A practical and conservative interaction equation combining shear and tensile allowables was developed and validated to evaluate an acceptable (safe) combination of tensile and shear stresses acting in the brazed joint. These allowables are obtained from testing of standard tensile and lap shear brazed specimens. The proposed equation enables the assessment of the load carrying capability of complex brazed joints subjected to multi-axial loading.

Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein*

PubMed Central

Klein, Gracjana; Kobylak, Natalia; Lindner, Buko; Stupak, Anna; Raina, Satish

2014-01-01

Here, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress-responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with an enrichment of LPS derivatives with truncations in the core region, some of which were pentaacylated and exhibited carbon chain polymorphism. Further, the levels of LpxC, the enzyme that catalyzes the first committed step of lipid A synthesis, were highly elevated in the Δ(lapA lapB) mutant. Δ(lapA lapB) mutant accumulated extragenic suppressors that mapped either to lpxC, waaC, and gmhA, or to the waaQ operon (LPS biosynthesis) and lpp (Braun's lipoprotein). Increased synthesis of either FabZ (3-R-hydroxymyristoyl acyl carrier protein dehydratase), slrA (novel RpoE-regulated non-coding sRNA), lipoprotein YceK, toxin HicA, or MurA (UDP-N-acetylglucosamine 1-carboxyvinyltransferase) suppressed some of the Δ(lapA lapB) defects. LapB contains six tetratricopeptide repeats and, at the C-terminal end, a rubredoxin-like domain that was found to be essential for its activity. In pull-down experiments, LapA and LapB co-purified with LPS, Lpt proteins, FtsH (protease), DnaK, and DnaJ (chaperones). A specific interaction was also observed between WaaC and LapB. Our data suggest that LapB coordinates assembly of proteins involved in LPS synthesis at the plasma membrane and regulates turnover of LpxC, thereby ensuring balanced biosynthesis of LPS and phospholipids consistent with its essentiality. PMID:24722986

The effect of viscoelasticity on the stress distribution of adhesively single-lap joint with an internal break in the composite adherends

NASA Astrophysics Data System (ADS)

Reza, Arash; Shishesaz, Mohammad

2017-09-01

The aim of this research is to study the effect of a break in the laminated composite adherends on stress distribution in the adhesively single-lap joint with viscoelastic adhesive and matrix. The proposed model involves two adherends with E-glass fibers and poly-methyl-methacrylate matrix that have been adhered to each other by phenolic-epoxy resin. The equilibrium equations that are based on shear-lag theory have been derived in the Laplace domain, and the governing differential equations of the model have been derived analytically in the Laplace domain. A numerical inverse Laplace transform, which is called Gaver-Stehfest method, has been used to extract desired results in the time domain. The results obtained at the initial time completely matched with the results of elastic solution. Also, a comparison between results obtained from the analytical and finite element models show a relatively good match. The results show that viscoelastic behavior decreases the peak of stress near the break. Finally, the effect of size and location of the break, as well as volume fraction of fibers, on the stress distribution in the adhesive layer is fully investigated.

Analytical and Numerical Results for an Adhesively Bonded Joint Subjected to Pure Bending

NASA Technical Reports Server (NTRS)

Smeltzer, Stanley S., III; Lundgren, Eric

2006-01-01

A one-dimensional, semi-analytical methodology that was previously developed for evaluating adhesively bonded joints composed of anisotropic adherends and adhesives that exhibit inelastic material behavior is further verified in the present paper. A summary of the first-order differential equations and applied joint loading used to determine the adhesive response from the methodology are also presented. The method was previously verified against a variety of single-lap joint configurations from the literature that subjected the joints to cases of axial tension and pure bending. Using the same joint configuration and applied bending load presented in a study by Yang, the finite element analysis software ABAQUS was used to further verify the semi-analytical method. Linear static ABAQUS results are presented for two models, one with a coarse and one with a fine element meshing, that were used to verify convergence of the finite element analyses. Close agreement between the finite element results and the semi-analytical methodology were determined for both the shear and normal stress responses of the adhesive bondline. Thus, the semi-analytical methodology was successfully verified using the ABAQUS finite element software and a single-lap joint configuration subjected to pure bending.

Influence of Zn Interlayer on Interfacial Microstructure and Mechanical Properties of TIG Lap-Welded Mg/Al Joints

NASA Astrophysics Data System (ADS)

Gao, Qiong; Wang, Kehong

2016-03-01

This study explored 6061 Al alloy and AZ31B Mg alloy joined by TIG lap welding with Zn foils of varying thicknesses, with the additional Zn element being imported into the fusion zone to alloy the weld seam. The microstructures and chemical composition in the fusion zone near the Mg substrate were examined by SEM and EDS, and tensile shear strength tests were conducted to investigate the mechanical properties of the Al/Mg joints, as well as the fracture surfaces, and phase compositions. The results revealed that the introduction of an appropriate amount of Zn transition layer improves the microstructure of Mg/Al joints and effectively reduces the formation of Mg-Al intermetallic compounds (IMCs). The most common IMCs in the fusion zone near the Mg substrate were Mg-Zn and Mg-Al-Zn IMCs. The type and distribution of IMCs generated in the weld zone differed according to Zn additions; Zn interlayer thickness of 0.4 mm improved the sample's mechanical properties considerably compared to thicknesses of less than 0.4 mm; however, any further increase in Zn interlayer thickness of above 0.4 mm caused mechanical properties to deteriorate.

Mineralogy, Petrology and Oxygen Fugacity of the LaPaz Icefield Lunar Basaltic Meteorites and the Origin of Evolved Lunar Basalts

NASA Technical Reports Server (NTRS)

Collins, S. J.; Righter, K.; Brandon, A. D.

2005-01-01

LAP 02205 is a 1.2 kg lunar mare basalt meteorite found in the Lap Paz ice field of Antarctica in 2002 [1]. Four similar meteorites were also found within the same region [1] and all five have a combined mass of 1.9 kg (LAP 02224, LAP 02226, LAP 02436 and LAP 03632, hereafter called the LAP meteorites). The LAP meteorites all contain a similar texture, mineral assemblage, and composition. A lunar origin for these samples comes from O isotopic data for LAP 02205 [1], Fe/Mn ratios of pyroxenes [1-5], and the presence of distinct lunar mineralogy such as Fe metal and baddeleyite. The LAP meteorites may represent an area of the Moon, which has never been sampled by Apollo missions, or by other lunar meteorites. The data from this study will be used to compare the LAP meteorites to Apollo mare basalts and lunar basaltic meteorites, and will ultimately help to constrain their origin.

Deformation measurement for a rotating deformable lap based on inverse fringe projection

NASA Astrophysics Data System (ADS)

Liao, Min; Zhang, Qican

2015-03-01

The active deformable lap (also namely stressed lap) is an efficient polishing tool in optical manufacturing. To measure the dynamic deformation caused by outside force on a deformable lap is important and helpful to the opticians to ensure the performance of a deformable lap as expected. In this paper, a manual deformable lap was designed to simulate the dynamic deformation of an active stressed lap, and a measurement system was developed based on inverse projected fringe technique to restore the 3D shape. A redesigned inverse fringe has been projected onto the surface of the measured lap, and the deformations of the tested lap become much obvious and can be easily and quickly evaluated by Fourier fringe analysis. Compared with the conventional projection, this technique is more obvious, and it should be a promising one in the deformation measurement of the active stressed lap in optical manufacturing.

One-shot and aberration-tolerable homodyne detection for holographic storage readout through double-frequency grating-based lateral shearing interferometry.

PubMed

Yu, Yeh-Wei; Xiao, Shuai; Cheng, Chih-Yuan; Sun, Ching-Cherng

2016-05-16

A simple method to decode the stored phase signal of volume holographic data storage with adequate wave aberration tolerance is highly demanded. We proposed and demonstrated a one-shot scheme to decode a binary-phase encoding signal through double-frequency-grating based shearing interferometry (DFGSI). The lateral shearing amount is dependent on the focal length of the collimated lens and the frequency difference between the gratings. Diffracted waves with phase encoding were successfully decoded through experimentation. An optical model for the DFGSI was built to analyze phase-error induction and phase-difference control by shifting the double-frequency grating longitudinally and laterally, respectively. The optical model was demonstrated experimentally. Finally, a high aberration tolerance of the DFGSI was demonstrated using the optical model.

Processing study of a high temperature adhesive

NASA Technical Reports Server (NTRS)

Progar, D. J.

1984-01-01

An adhesive-bonding process cycle study was performed for a polyimidesulphone. The high molecular weight, linear aromatic system possesses properties which make it attractive as a processable, low-cost material for elevated temperature applications. The results of a study to better understand the parameters that affect the adhesive properties of the polymer for titanium alloy adherends are presented. These include the tape preparation, the use of a primer and press and simulated autoclave processing conditions. The polymer was characterized using Fourier transform infrared spectroscopy, glass transition temperature determination, flow measurements, and weight loss measurements. The lap shear strength of the adhesive was used to evaluate the effects of the bonding process variations.

The effect of elastomer chain length on properties of silicone-modified polyimide adhesives

NASA Technical Reports Server (NTRS)

St.clair, A. K.; St.clair, T. L.; Ezzell, S.

1981-01-01

A series of polyimides containing silicone elastomers was synthesized in order to study the effects of the elastomer chain length on polymer properties. The elastomer with repeat units varying from n=10 to 105 was chemically reacted into the backbone of an addition polyimide oligomer via reactive aromatic amine groups. Glass transition temperatures of the elastomer and polyimide phases were observed by torsional braid analysis. The elastomer-modified polyimides were tested as adhesives for bonding titanium in order to determine their potential for aerospace applications. Adhesive lap shear tests were performed before and after aging bonded specimens at elevated temperatures.

Adhesive Properties of Cured Phenylethynyl containing Imides

NASA Technical Reports Server (NTRS)

Chang, Alice C.

1997-01-01

As part of a program to develop structural adhesives for high performance aerospace applications, several phenylethynyl containing oligomer blends of Larc(TM) MPEI and a reactive plasticizer designated LaRC LV-1 21 were prepared and evaluated. The fully imidized blends exhibited minimum melt viscosity as low as 1000 poise at 371 C. Ti/Ti lap shear specimens fabricated at 316 C under 15 psi gave RT strength of approx. 4300 psi and no change in strength was observed at 177 C. The chemistry and properties of this new MPEI as well as some blends of MPEI with LV-121 are presented and compared to the linear version, LARC(TM)-PETI-5.

Improved simulation method of automotive spot weld failure with an account of the mechanical properties of spot welds

NASA Astrophysics Data System (ADS)

Wu, H.; Meng, X. M.; Fang, R.; Huang, Y. F.; Zhan, S.

2017-12-01

In this paper, the microstructure and mechanical properties of spot weld were studied, the hardness of nugget and heat affected zone (HAZ) were also tested by metallographic microscope and microhardness tester. The strength of the spot weld with the different parts' area has been characterized. According to the experiments result, CAE model of spot weld with HAZ structure was established, and simulation results of different lap-shear CAE models were analyzed. The results show that the spot weld model which contained the HAZ has good performance and more suitable for engineering application in spot weld simulation.

The induction of tomato leucine aminopeptidase genes (LapA) after Pseudomonas syringae pv. tomato infection is primarily a wound response triggered by coronatine.

PubMed

Pautot, V; Holzer, F M; Chaufaux, J; Walling, L L

2001-02-01

Tomato plants constitutively express a neutral leucine aminopeptidase (LAP-N) and an acidic LAP (LAP-A) during floral development and in leaves in response to insect infestation, wounding, and Pseudomonas syringae pv. tomato infection. To assess the physiological roles of LAP-A, a LapA-antisense construct (35S:asLapA1) was introduced into tomato. The 35S:asLapA1 plants had greatly reduced or showed undetectable levels of LAP-A and LAP-N proteins in healthy and wounded leaves and during floral development. Despite the loss of these aminopeptidases, no global changes in protein profiles were noted. The 35S:asLapA1 plants also exhibited no significant alteration in floral development and did not impact the growth and development of Manduca sexta and P. syringae pv. tomato growth rates during compatible or incompatible infections. To investigate the mechanism underlying the strong induction of LapA upon P. syringae pv. tomato infection, LapA expression was monitored after infection with coronatine-producing and -deficient P. syringae pv. tomato strains. LapA RNA and activity were detected only with the coronatine-producing P. syringae pv. tomato strain. Coronatine treatment of excised shoots caused increases in RNAs for jasmonic acid (JA)-regulated wound-response genes (LapA and pin2) but did not influence expression of a JA-regulated pathogenesis-related protein gene (PR-1). These results indicated that coronatine mimicked the wound response but was insufficient to activate JA-regulated PR genes.

Full-field local displacement analysis of two-sided paperboard

Treesearch

J.M. Considine; D.W. Vahey

2007-01-01

This report describes a method to examine full-field displacements of both sides of paperboard during tensile testing. Analysis showed out-of-plane shear behavior near the failures zones. The method was reliably used to examine out-of-plane shear in double notch shear specimens. Differences in shear behavior of machine direction and cross-machine direction specimens...

Mechanical shear and tensile characteristics of selected biomass stems

USDA-ARS?s Scientific Manuscript database

Mechanical characteristics (stress and energy of tensile and shear modes) of selected biomass stems, such as big bluestem, bromegrass, and Barlow wheat were determined. A high capacity MTI-100K universal testing machine attached with standard tensile clamps and designed fabricated double-shear devic...

Lapping slurry

DOEpatents

Simandl, Ronald F.; Upchurch, Victor S.; Leitten, Michael E.

1999-01-01

Improved lapping slurries provide for easier and more thorough cleaning of alumina workpieces, as well as inhibit corrosion of the lapping table and provide for easier cleaning of the lapping equipment. The unthickened lapping slurry comprises abrasive grains such as diamond abrasive dispersed in a carrier comprising water, glycerine, and triethanolamine. The thickened lapping slurry comprises abrasive grains such as diamond abrasive dispersed in a carrier comprising water, glycerine, triethanolamine, a water soluble silicate, and acid.

In vitro modulation of the interaction between HA95 and LAP2beta by cAMP signaling.

PubMed

Martins, Sandra B; Marstad, Anne; Collas, Philippe

2003-09-09

The nuclear envelope mediates key functions by interacting with chromatin. We recently reported an interaction between the chromatin- and nuclear matrix-associated protein HA95 and the inner nuclear membrane integral protein LAP2beta, implicated in initiation of DNA replication (Martins et al. (2003) J. Cell Biol. 160, 177-188). Here, we show that in vitro, interaction between HA95 and LAP2beta is modulated by cAMP signaling via PKA. Exposure of an anti-HA95 immune precipitate from interphase HeLa cells to a mitotic extract promotes ATP-dependent release of LAP2beta from the HA95 complex. This coincides with Ser and Thr phosphorylation of HA95 and LAP2beta. Inhibition of PKA with PKI abolishes phosphorylation of HA95 and dissociation of LAP2beta from HA95, although LAPbeta remains phosphorylated. Antagonizing cAMP signaling in mitotic extract also abolishes the release of LAP2beta from HA95; however, disrupting PKA anchoring to A-kinase anchoring proteins has no effect. Inhibition of CDK activity in the extract greatly reduces LAP2beta phosphorylation but does not prevent LAP2beta release from HA95. Inhibition of PKC, MAP kinase, or CaM kinase II does not affect mitotic extract-induced dissociation of LAP2beta from HA95. PKA phosphorylates HA95 but not LAP2beta in vitro and elicits a release of LAP2beta from HA95. CDK1 or PKC phosphorylates LAP2beta within the HA95 complex, but neither kinase induces LAP2beta release. Our results indicate that in vitro, the interaction between HA95 and LAP2beta is influenced by a PKA-mediated phosphorylation of HA95 rather than by CDK1- or PKC-mediated phosphorylation of LAP2beta. This suggests an additional level of regulation of a chromatin-nuclear envelope interaction in dividing cells.

Lapping slurry

DOEpatents

Simandl, R.F.; Upchurch, V.S.; Leitten, M.E.

1999-01-05

Improved lapping slurries provide for easier and more thorough cleaning of alumina work pieces, as well as inhibit corrosion of the lapping table and provide for easier cleaning of the lapping equipment. The unthickened lapping slurry comprises abrasive grains such as diamond abrasive dispersed in a carrier comprising water, glycerine, and triethanolamine. The thickened lapping slurry comprises abrasive grains such as diamond abrasive dispersed in a carrier comprising water, glycerine, triethanolamine, a water soluble silicate, and acid. 1 fig.

The pressure control technology of the active stressed lap

NASA Astrophysics Data System (ADS)

Li, Ying; Wang, Daxing

2010-10-01

The active stressed lap polishing technology is a kind of new polishing technology that can actively deform the lap surface to become an off-axis asphere according to different lap position on mirror surface and different angle of lap. The pressure of the lap on the mirror is an important factor affecting the grinding efficiency of the optics mirror. The active stressed lap technology using dynamic pressure control solution in the process of polishing astronomical Aspheric Mirror with faster asphericity will provide the advantage like high polishing speed and natural smooth, etc. This article puts emphases on the pressure control technology of the active stressed lap technology. It requires that the active stressed lap keeps symmetrical vertical compression on the mirrors in the process of grinding mirrors. With a background of an active stressed lap 450mm in diameter, this article gives an outline of the pressure control organization, analyzes the principle of pressure control and proposes the limitations of the present pressure control organization and the relevant solutions, designs a digital pressure controller with C32-bit RISC embedded and gives the relevant experimental test result finally.

Subsurface damage distribution in the lapping process.

PubMed

Wang, Zhuo; Wu, Yulie; Dai, Yifan; Li, Shengyi

2008-04-01

To systematically investigate the influence of lapping parameters on subsurface damage (SSD) depth and characterize the damage feature comprehensively, maximum depth and distribution of SSD generated in the optical lapping process were measured with the magnetorheological finishing wedge technique. Then, an interaction of adjacent indentations was applied to interpret the generation of maximum depth of SSD. Eventually, the lapping procedure based on the influence of lapping parameters on the material removal rate and SSD depth was proposed to improve the lapping efficiency.

Abernathy's Lap

NASA Technical Reports Server (NTRS)

1977-01-01

A lap in this instance is not a midriff but a tool for presision.polishing and grinding. During the Saturn V moonbooster program, Marshall Space Flight Center found a need for a better lap. The need arose from the exquisitely precise tolerances required for parts of the launch vehicle's guidance,and control system. So William J. Abernathy, a former Marshall employee, built a better lap; he invented a method for charging aluminum lap plates with diamond powder, then hard-anodizing them. The resulting lap produces a high polish on materials ranging from the softest aluminum to the hardest ceramics. It operates faster, wears longer and requires less reworking. Abernathy got NASA's permission to obtain a personal patent and he formed the one-man Abernathy Laps Co. in Huntsville, which produces a variety of laps. One of Abernathy's customers is Bell Aerospace Textron, Buffalo, which uses the laps to finish polish delicate instrument parts produced for NASA's Viking and other space programs. Says a Bell official: "Time needed (with the Abernathy lap) is a fraction of that required by conventional methods. The result is extremely accurate flatness and surface finish." Abernathy is providing laps for other manufacturing applications and for preparation of metallurgical specimens. The business is small but steady, and Abernathy plans expansion into other markets.

Pressure variation of developed lapping tool on surface roughness

NASA Astrophysics Data System (ADS)

Hussain, A. K.; Lee, K. Q.; Aung, L. M.; Abu, A.; Tan, L. K.; Kang, H. S.

2018-01-01

Improving the surface roughness is always one of the major concerns in the development of lapping process as high precision machining caters a great demand in manufacturing process. This paper aims to investigate the performance of a newly designed lapping tool in term of surface roughness. Polypropylene is used as the lapping tool head. The lapping tool is tested for different pressure to identify the optimum working pressure for lapping process. The theoretical surface roughness is also calculated using Vickers Hardness. The present study shows that polypropylene is able to produce good quality and smooth surface roughness. The optimum lapping pressure in the present study is found to be 45 MPa. By comparing the theoretical and experimental values, the present study shows that the newly designed lapping tool is capable to produce finer surface roughness.

Additive manufacturing of tools for lapping glass

NASA Astrophysics Data System (ADS)

Williams, Wesley B.

2013-09-01

Additive manufacturing technologies have the ability to directly produce parts with complex geometries without the need for secondary processes, tooling or fixtures. This ability was used to produce concave lapping tools with a VFlash 3D printer from 3D Systems. The lapping tools were first designed in Creo Parametric with a defined constant radius and radial groove pattern. The models were converted to stereolithography files which the VFlash used in building the parts, layer by layer, from a UV curable resin. The tools were rotated at 60 rpm and used with 120 grit and 220 grit silicon carbide lapping paste to lap 0.750" diameter fused silica workpieces. The samples developed a matte appearance on the lapped surface that started as a ring at the edge of the workpiece and expanded to the center. This indicated that as material was removed, the workpiece radius was beginning to match the tool radius. The workpieces were then cleaned and lapped on a second tool (with equivalent geometry) using a 3000 grit corundum aluminum oxide lapping paste, until a near specular surface was achieved. By using lapping tools that have been additively manufactured, fused silica workpieces can be lapped to approach a specified convex geometry. This approach may enable more rapid lapping of near net shape workpieces that minimize the material removal required by subsequent polishing. This research may also enable development of new lapping tool geometry and groove patterns for improved loose abrasive finishing.

Overexpression and characterization of an extracellular leucine aminopeptidase from Aspergillus oryzae.

PubMed

Matsushita-Morita, Mayumi; Tada, Sawaki; Suzuki, Satoshi; Hattori, Ryota; Marui, Junichiro; Furukawa, Ikuyo; Yamagata, Youhei; Amano, Hitoshi; Ishida, Hiroki; Takeuchi, Michio; Kashiwagi, Yutaka; Kusumoto, Ken-Ichi

2011-02-01

Leucine aminopeptidase (LAP), an enzyme used in the food industry, is an exopeptidase that removes an amino acid residue, primarily leucine (Leu), from the N-terminus of peptides and protein substrates. In this study, we focused on the leucine aminopeptidase A (lapA) gene from Aspergillus oryzae RIB40. To purify and characterize the LapA, lapA was overexpressed in A. oryzae RIB40 using the amyB promoter. LAP activity in the culture supernatant of one transformant harboring the lapA expression plasmid was 33 times that of the host strain. LapA was purified from the culture supernatant of this lapA-overexpressing strain by column chromatography. The purified recombinant LapA had a molecular mass of 33 kDa, and its N-terminal amino acid was the tyrosine at position 80 of the deduced amino acid sequence. Optimal enzyme activity was observed at 60°C and pH 8.5, and the enzyme was stable at temperatures up to 60°C and in the pH range 7.5-11. In transcriptional analysis, lapA was induced under alkaline conditions and expressed at a relatively low level under normal conditions. LapA showed maximum hydrolyzing activity for the substrate leucine para-nitroanilide (Leu-pNA), followed by substrates Phe-pNA (39% activity compared with Leu-pNA), Met-pNA, Lys-pNA, and Arg-pNA. In addition, LapA preferentially hydrolyzed peptides longer than tripeptides.

Alterations in the Helicoverpa armigera Midgut Digestive Physiology after Ingestion of Pigeon Pea Inducible Leucine Aminopeptidase

PubMed Central

Lomate, Purushottam R.; Jadhav, Bhakti R.; Giri, Ashok P.; Hivrale, Vandana K.

2013-01-01

Jasmonate inducible plant leucine aminopeptidase (LAP) is proposed to serve as direct defense in the insect midgut. However, exact functions of inducible plant LAPs in the insect midgut remain to be estimated. In the present investigation, we report the direct defensive role of pigeon pea inducible LAP in the midgut of Helicoverpa armigera (Lepidoptera: Noctuidae) and responses of midgut soluble aminopeptidases and serine proteinases upon LAP ingestion. Larval growth and survival was significantly reduced on the diets supplemented with pigeon pea LAP. Aminopeptidase activities in larvae remain unaltered in presence or absence of inducible LAP in the diet. On the contrary, serine proteinase activities were significantly decreased in the larvae reared on pigeon pea LAP containing diet as compared to larvae fed on diet without LAP. Our data suggest that pigeon pea inducible LAP is responsible for the degradation of midgut serine proteinases upon ingestion. Reduction in the aminopeptidase activity with LpNA in the H. armigera larvae was compensated with an induction of aminopeptidase activity with ApNA. Our findings could be helpful to further dissect the roles of plant inducible LAPs in the direct plant defense against herbivory. PMID:24098675

Alterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.

PubMed

Lomate, Purushottam R; Jadhav, Bhakti R; Giri, Ashok P; Hivrale, Vandana K

2013-01-01

Jasmonate inducible plant leucine aminopeptidase (LAP) is proposed to serve as direct defense in the insect midgut. However, exact functions of inducible plant LAPs in the insect midgut remain to be estimated. In the present investigation, we report the direct defensive role of pigeon pea inducible LAP in the midgut of Helicoverpa armigera (Lepidoptera: Noctuidae) and responses of midgut soluble aminopeptidases and serine proteinases upon LAP ingestion. Larval growth and survival was significantly reduced on the diets supplemented with pigeon pea LAP. Aminopeptidase activities in larvae remain unaltered in presence or absence of inducible LAP in the diet. On the contrary, serine proteinase activities were significantly decreased in the larvae reared on pigeon pea LAP containing diet as compared to larvae fed on diet without LAP. Our data suggest that pigeon pea inducible LAP is responsible for the degradation of midgut serine proteinases upon ingestion. Reduction in the aminopeptidase activity with LpNA in the H. armigera larvae was compensated with an induction of aminopeptidase activity with ApNA. Our findings could be helpful to further dissect the roles of plant inducible LAPs in the direct plant defense against herbivory.

Structural Features of the Pseudomonas fluorescens Biofilm Adhesin LapA Required for LapG-Dependent Cleavage, Biofilm Formation, and Cell Surface Localization

PubMed Central

Boyd, Chelsea D.; Smith, T. Jarrod; El-Kirat-Chatel, Sofiane; Newell, Peter D.; Dufrêne, Yves F.

2014-01-01

The localization of the LapA protein to the cell surface is a key step required by Pseudomonas fluorescens Pf0-1 to irreversibly attach to a surface and form a biofilm. LapA is a member of a diverse family of predicted bacterial adhesins, and although lacking a high degree of sequence similarity, family members do share common predicted domains. Here, using mutational analysis, we determine the significance of each domain feature of LapA in relation to its export and localization to the cell surface and function in biofilm formation. Our previous work showed that the N terminus of LapA is required for cleavage by the periplasmic cysteine protease LapG and release of the adhesin from the cell surface under conditions unfavorable for biofilm formation. We define an additional critical region of the N terminus of LapA required for LapG proteolysis. Furthermore, our results suggest that the domains within the C terminus of LapA are not absolutely required for biofilm formation, export, or localization to the cell surface, with the exception of the type I secretion signal, which is required for LapA export and cell surface localization. In contrast, deletion of the central repetitive region of LapA, consisting of 37 repeats of 100 amino acids, results in an inability to form a biofilm. We also used single-molecule atomic force microscopy to further characterize the role of these domains in biofilm formation on hydrophobic and hydrophilic surfaces. These studies represent the first detailed analysis of the domains of the LapA family of biofilm adhesin proteins. PMID:24837291

Numeric and fluid dynamic representation of tornadic double vortex thunderstorms

NASA Technical Reports Server (NTRS)

Connell, J. R.; Marquart, E. J.; Frost, W.; Boaz, W.

1980-01-01

Current understanding of a double vortex thunderstorm involves a pair of contra-rotating vortices that exists in the dynamic updraft. The pair is believed to be a result of a blocking effect which occurs when a cylindrical thermal updraft of a thunderstorm protrudes into the upper level air and there is a large amount of vertical wind shear between the low level and upper level air layers. A numerical tornado prediction scheme based on the double vortex thunderstorm was developed. The Energy-Shear Index (ESI) is part of the scheme and is calculated from radiosonde measurements. The ESI incorporates parameters representative of thermal instability and blocking effect, and indicates appropriate environments for which the development of double vortex thunderstorms is likely.

Numerical modeling and experimental validation of thermoplastic composites induction welding

NASA Astrophysics Data System (ADS)

Palmieri, Barbara; Nele, Luigi; Galise, Francesco

2018-05-01

In this work, a numerical simulation and experimental test of the induction welding of continuous fibre-reinforced thermoplastic composites (CFRTPCs) was provided. The thermoplastic Polyamide 66 (PA66) with carbon fiber fabric was used. Using a dedicated software (JMag Designer), the influence of the fundamental process parameters such as temperature, current and holding time was investigated. In order to validate the results of the simulations, and therefore the numerical model used, experimental tests were carried out, and the temperature values measured during the tests were compared with the aid of an optical pyrometer, with those provided by the numerical simulation. The mechanical properties of the welded joints were evaluated by single lap shear tests.

Evaluation of high temperature structural adhesives for extended service, phase 4

NASA Technical Reports Server (NTRS)

Hendricks, C. L.; Hill, S. G.; Hale, J. N.

1985-01-01

The evaluation of three phenylquinoxaline polymers as high temperature structural adhesives is presented. These included an experimental crisskubjabke oiktner (X-PQ) and two experimental materials (PPQ-2501) and (PPQ-HC). Lap shear, crack extension, and climing drum peel specimens were fabricated from all three polymers, and tested after thermal, combined thermal/humidity, and stressed Skydrol exposure. All three polymers generally performed well as adhesives at initial test temperatures from 219K (-67 F) to 505K (450 F) and after humidity exposure. The 644K (700 F) cured test specimens exhibited superior Skydrol resistance and thermal stability at 505K (450 F) when compared to the 602K (625 F) cured test specimens.

Friction Stir Spot Welding of Advanced High Strength Steels

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hovanski, Yuri; Grant, Glenn J.; Santella, M. L.

Friction stir spot welding techniques were developed to successfully join several advanced high strength steels. Two distinct tool materials were evaluated to determine the effect of tool materials on the process parameters and joint properties. Welds were characterized primarily via lap shear, microhardness, and optical microscopy. Friction stir spot welds were compared to the resistance spot welds in similar strength alloys by using the AWS standard for resistance spot welding high strength steels. As further comparison, a primitive cost comparison between the two joining processes was developed, which included an evaluation of the future cost prospects of friction stir spotmore » welding in advanced high strength steels.« less

Laser surface texturing of polypropylene to increase adhesive bonding

NASA Astrophysics Data System (ADS)

Mandolfino, Chiara; Pizzorni, Marco; Lertora, Enrico; Gambaro, Carla

2018-05-01

In this paper, the main parameters of laser surface texturing of polymeric substrates have been studied. The final aim of the texturing is to increase the performance of bonded joints of grey-pigmented polypropylene substrates. The experimental investigation was carried out starting from the identification of the most effective treatment parameters, in order to achieve a good texture without compromising the characteristics of the bulk material. For each of these parameters, three values were individuated and 27 sets of samples were realised. The surface treatment was analysed and related to the mechanical characteristics of the bonded joints performing lap-shear tests. A statistical analysis in order to find the most influential parameter completed the work.

Investigation of problems associated with solid encapsulation of high voltage electronic assemblies; also Reynolds connector study

NASA Technical Reports Server (NTRS)

Bever, R. S.

1975-01-01

Electric breakdown prevention in vacuum and encapsulation of high voltage electronic circuits was studied. The lap shear method was used to measure adhesive strengths. The permeation constants of air at ambient room temperature through four different space-grade encapsulants was measured. Order of magnitude was calculated for the time that air bubble pressures drop to the corona region. High voltage connectors with L-type cable attached were tested in a vacuum system at various pressures. The cable system was shown to suppress catastrophic breakdown when filled with and surrounded by gas in the corona region of pressures, but did not prove to be completely noise free.

Joining thick section aluminum to steel with suppressed FeAl intermetallic formation via friction stir dovetailing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reza-E-Rabby, Md.; Ross, Kenneth; Overman, Nicole R.

A new solid-phase technique called friction stir dovetailing (FSD) has been developed for joining thick section aluminum to steel. In FSD, mechanical interlocks are formed at the aluminum-steel interface and are reinforced by metallurgical bonds where intermetallic growth has been uniquely suppressed. Lap shear testing shows superior strength and extension at failure compared to popular friction stir approaches where metallurgical bonding is the only joining mechanism. High resolution microscopy revealed the presence of a 40-70 nm interlayer having a composition of 76.4 at% Al, 18.4 at% Fe, and 5.2 at% Si, suggestive of limited FeAl3 intermetallic formation.

PMR-15 polyimide modifications for improved prepreg tack

NASA Technical Reports Server (NTRS)

Vannucci, R. D.

1982-01-01

The use of mixed solvents and of modified monomeric ester reactants was investigated as a means of improving the tack and drape retention characteristics of PMR-15 polyimide prepreg. Methanol, ethanol, 1-propanol and 1-butanol were used to prepare the esters, prepreg solutions, and T-300 graphite fabric and Celion 6000 unidirectional fiber prepregs. The tack retention characteristics of the T-300 fabric prepreg after exposure to simulated use conditions were determined using a simple lap shear test. Drape was qualitatively assessed by visually monitoring the deformability of the prepreg. Thermo-oxidative stability and mechanical properties retention of the Celion 6000 grahite fiber composites were determined as a function of exposure time in air at 600 F.

Laser Surface Preparation for Adhesive Bonding of Ti-6Al-4V

NASA Technical Reports Server (NTRS)

Belcher, Marcus A.; List, Martina S.; Wohl, Christopher J.; Ghose, Sayata; Watson, Kent A.; Hopkins, John W.; Connell, John W.

2010-01-01

Adhesively bonded structures are potentially lighter in weight than mechanically fastened ones, but existing surface treatments are often considered unreliable. Two main problems in achieving reproducible and durable adhesive bonds are surface contamination and variability in standard surface preparation techniques. In this work three surface pretreatments were compared: laser etching with and without grit blasting and conventional Pasa-Jell treatment. Ti-6Al-4V surfaces were characterized by contact angle goniometry, optical microscopy, and X-ray photoelectron spectroscopy (XPS). Laser -etching was found to produce clean surfaces with precisely controlled surface topographies and PETI-5 lap shear strengths and durabilities were equivalent to those produced with Pasa-Jell.

Design, fabrication, installation and flight service evaluation of a composite cargo ramp skin on a model CH-53 helicopter

NASA Technical Reports Server (NTRS)

Lowry, D. W.; Rich, M. J.

1983-01-01

The installation of a composite skin panel on the cargo ramp of a CH-530 marine helicopter is discussed. The composite material is of Kevlar/Epoxy (K/E) which replaces aluminum outer skins on the aft two bays of the ramp. The cargo ramp aft region was selected as being a helicopter airframe surface subjected to possible significant field damage and would permit an evaluation of the long term durability of the composite skin panel. A structural analysis was performed and the skin shears determined. Single lap joints of K/E riveted to aluminum were statically tested. The joint tests were used to determine bearing allowables and the required K/E skin gage. The K/E skin panels riveted to aluminum edge members were tested in a shear fixture to confirm the allowable shear and bearing strengths. Impact tests were conducted on aluminum skin panels to determine energy level and damage relationship. The K/E skin panels of various ply orientations and laminate thicknesses were then impacted at similar energy levels. The results of the analysis and tests were used to determine the required K/E skin gages in each of the end two bays of the ramp.

Numerical study on tilting salt finger in a laminar shear flow

NASA Astrophysics Data System (ADS)

Zhang, Xianfei; Wang, Ling-ling; Lin, Cheng; Zhu, Hai; Zeng, Cheng

2018-02-01

Salt fingers as a mixing mechanism in the ocean have been investigated for several decades, together with a key issue being focused on their convective evolution and flux ratio variation. However, related studies on tilting fingers in the ocean produced by shear flow have been ignored by previous researchers. In this paper, a 2-D numerical model is presented to study the evolution of the double-diffusion salt finger in a two-layer thermohaline system with laminar shear flow. The model is divided into a steady-state solver and double-diffusion convection system, aimed to reveal the effect of shear flow on salt fingers and analyze the mechanism behind the shear and fingers. Several cases are conducted for Re = 0 ˜ 900 to study the evolution of salt fingers in a laminar shear flow and the variation of salt flux with Re. The results show that salt fingers exist and tilt in the presence of laminar shear flow. The mass transport in the vertical direction is weakened as the Reynolds number increases. An asymmetric structure of the salt finger is discovered and accounts for the morphological tilt and salt flux reduction.

Role of LAP+CD4+ T cells in the tumor microenvironment of colorectal cancer.

PubMed

Zhong, Wu; Jiang, Zhi-Yuan; Zhang, Lei; Huang, Jia-Hao; Wang, Shi-Jun; Liao, Cun; Cai, Bin; Chen, Li-Sheng; Zhang, Sen; Guo, Yun; Cao, Yun-Fei; Gao, Feng

2017-01-21

To investigate the abundance and potential functions of LAP + CD4 + T cells in colorectal cancer (CRC). Proportions of LAP + CD4 + T cells were examined in peripheral blood and tumor/paratumor tissues of CRC patients and healthy controls using flow cytometry. Expression of phenotypic markers such as forkhead box (Fox)p3, cytotoxic T-lymphocyte-associated protein (CTLA)-4, chemokine CC receptor (CCR)4 and CCR5 was measured using flow cytometry. LAP - CD4 + and LAP + CD4 + T cells were isolated using a magnetic cell-sorting system and cell purity was analyzed by flow cytometry. Real-time quantitative polymerase chain reaction was used to measure expression of cytokines interleukin (IL)-10 and transforming growth factor (TGF)-β. The proportion of LAP + CD4 + T cells was significantly higher in peripheral blood from patients (9.44% ± 3.18%) than healthy controls (1.49% ± 1.00%, P < 0.001). Among patients, the proportion of LAP + CD4 + T cells was significantly higher in tumor tissues (11.76% ± 3.74%) compared with paratumor tissues (3.87% ± 1.64%, P < 0.001). We also observed positive correlations between the proportion of LAP + CD4 + T cells and TNM stage ( P < 0.001), distant metastasis ( P < 0.001) and serum level of carcinoembryonic antigen ( P < 0.05). Magnetic-activated cell sorting gave an overall enrichment of LAP + CD4 + T cells (95.02% ± 2.87%), which was similar for LAP - CD4 + T cells (94.75% ± 2.76%). In contrast to LAP - CD4 + T cells, LAP + CD4 + T cells showed lower Foxp3 expression but significantly higher levels of CTLA-4, CCR4 and CCR5 ( P < 0.01). LAP + CD4 + T cells expressed significantly larger amounts of IL-10 and TGF-β but lower levels of IL-2, IL-4, IL-17 and interferon-γ, compared with LAP - CD4 + T cells. LAP + CD4 + T cells accumulated in the tumor microenvironment of CRC patients and were involved in immune evasion mediated by IL-10 and TGF-β.

Pacing Behavior and Tactical Positioning in 1500-m Short-Track Speed Skating.

PubMed

Konings, Marco J; Noorbergen, Olaf S; Parry, David; Hettinga, Florentina J

2016-01-01

To gain more insight in pacing behavior and tactical positioning in 1500-m short-track speed skating, a sport in which several athletes directly compete in the same race. Lap times and intermediate rankings of elite 1500-m short-track- skating competitors were collected over the season 2012-13 (N = 510, 85 races). Two statistical approaches were used to assess pacing behavior and tactical positioning. First, lap times were analyzed using a MANOVA, and for each lap differences between sex, race type, final rankings, and stage of competition were determined. Second, Kendall tau b correlations were used to assess relationships between intermediate and final rankings. In addition, intermediate rankings of the winner of each race were examined. In 1500 m (13.5 laps of 111.12 m), correlations between intermediate and final ranking gradually increased throughout the race (eg, lap 1, r = .05; lap 7, r = .26; lap 13, r = .85). Moreover, the percentage of race winners skating in the leading position was over 50% during the last 3 laps. Top finishers were faster than bottom-place finishers only during the last 5 laps, with on average 0.1- to 1.5-s faster lap times of the race winners compared with the others during the last 5 laps. Although a fast start led to faster finishing times, top finishers were faster than bottom-placed finishers only during the last 5 laps. Moreover, tactical positioning at 1 of the foremost positions during the latter phase of the race appeared to be a strong determinant of finishing position.

Effect of Multiple Reflow Cycles and Al2O3 Nanoparticles Reinforcement on Performance of SAC305 Lead-Free Solder Alloy

NASA Astrophysics Data System (ADS)

Tikale, Sanjay; Prabhu, K. Narayan

2018-05-01

The effect of Al2O3 nanoparticles reinforcement on melting behavior, microstructure evolution at the interface and joint shear strength of 96.5Sn3Ag0.5Cu (SAC305) lead-free solder alloy subjected to multiple reflow cycles was investigated. The reinforced SAC305 solder alloy compositions were prepared by adding Al2O3 nanoparticles in different weight fractions (0.05, 0.1, 0.3 and 0.5 wt.%) through mechanical dispersion. Cu/solder/Cu micro-lap-shear solder joint specimens were used to assess the shear strength of the solder joint. Differential scanning calorimetry was used to investigate the melting behavior of SAC305 solder nanocomposites. The solder joint interfacial microstructure was studied using scanning electron microscopy. The results showed that the increase in melting temperature (T L) and melting temperature range of the SAC305 solder alloy by addition of Al2O3 nanoparticles were not significant. In comparison with unreinforced SAC305 solder alloy, the reinforcement of 0.05-0.5 wt.% of Al2O3 nanoparticles improved the solder wettability. The addition of nanoparticles in minor quantity effectively suppressed the Cu6Sn5 IMC growth, improved the solder joint shear strength and ductility under multiple reflow cycles. However, the improvement in solder properties was less pronounced on increasing the nanoparticle content above 0.1 wt.% of the solder alloy.

Scattering of plane transverse waves by spherical inclusions in a poroelastic medium

NASA Astrophysics Data System (ADS)

Liu, Xu; Greenhalgh, Stewart; Zhou, Bing

2009-03-01

The scattering of plane transverse waves by a spherical inclusion embedded in an infinite poroelastic medium is treated for the first time in this paper. The vector displacement wave equations of Biot's theory are solved as an infinite series of vector spherical harmonics for the case of a plane S-wave impinging from a porous medium onto a spherical inclusion which itself is assumed to be another porous medium. Based on the single spherical scattering theory and dynamic composite elastic medium theory, the non-self-consistent shear wavenumber is derived for a porous rock having numerous spherical inclusions of another medium. The frequency dependences of the shear wave velocity and the shear wave attenuation have been calculated for both the patchy saturation model (inclusions having the same solid frame as the host but with a different pore fluid from the host medium) and the double porosity model (inclusions having a different solid frame than the host but the same pore fluid as the host medium) with dilute concentrations of identical inclusions. Unlike the case of incident P-wave scattering, we show that although the fluid and the heterogeneity of the rock determine the shear wave velocity of the composite, the attenuation of the shear wave caused by scattering is actually contributed by the heterogeneity of the rock for spherical inclusions. The scattering of incident shear waves in the patchy saturation model is quite different from that of the double porosity model. For the patchy saturation model, the gas inclusions do not significantly affect the shear wave dispersion characteristic of the water-filled host medium. However, the softer inclusion with higher porosity in the double porosity model can cause significant shear wave scattering attenuation which occurs at a frequency at which the wavelength of the shear wave is approximately equal to the characteristic size of the inclusion and depends on the volume fraction. Compared with analytic formulae for the low frequency limit of the shear velocity, our scattering model yields discrepancies within 4.0 per cent. All calculated shear velocities of the composite medium with dilute inclusion concentrations approach the high frequency limit of the host material.

Even between-lap pacing despite high within-lap variation during mountain biking.

PubMed

Martin, Louise; Lambeth-Mansell, Anneliese; Beretta-Azevedo, Liane; Holmes, Lucy A; Wright, Rachel; St Clair Gibson, Alan

2012-09-01

Given the paucity of research on pacing strategies during competitive events, this study examined changes in dynamic high-resolution performance parameters to analyze pacing profiles during a multiple-lap mountain-bike race over variable terrain. A global-positioning-system (GPS) unit (Garmin, Edge 305, USA) recorded velocity (m/s), distance (m), elevation (m), and heart rate at 1 Hz from 6 mountain-bike riders (mean±SD age=27.2±5.0 y, stature=176.8±8.1 cm, mass=76.3±11.7 kg, VO2max=55.1±6.0 mL·kg(-1)·min1) competing in a multilap race. Lap-by-lap (interlap) pacing was analyzed using a 1-way ANOVA for mean time and mean velocity. Velocity data were averaged every 100 m and plotted against race distance and elevation to observe the presence of intralap variation. There was no significant difference in lap times (P=.99) or lap velocity (P=.65) across the 5 laps. Within each lap, a high degree of oscillation in velocity was observed, which broadly reflected changes in terrain, but high-resolution data demonstrated additional nonmonotonic variation not related to terrain. Participants adopted an even pace strategy across the 5 laps despite rapid adjustments in velocity during each lap. While topographical and technical variations of the course accounted for some of the variability in velocity, the additional rapid adjustments in velocity may be associated with dynamic regulation of self-paced exercise.

Upregulation of NAD(P)H:Quinone Oxidoreductase By Radiation Potentiates the Effect of Bioreductive β-Lapachone on Cancer Cells1

PubMed Central

Choi, Eun K; Terai, Kaoru; Ji, In-Mi; Kook, Yeon H; Park, Kyung H; Oh, Eun T; Griffin, Robert J; Lim, Byung U; Kim, Jin-Seok; Lee, Doo S; Boothman, David A; Loren, Melissa; Song, Chang W; Park, Heon Joo

2007-01-01

We found that β-lapachone (β-lap), a novel bioreductive drug, caused rapid apoptosis and clonogenic cell death in A549 human lung epithelial cancer cells in vitro in a dose-dependent manner. The clonogenic cell death caused by β-lap could be significantly inhibited by dicoumarol, an inhibitor of NAD(P)H:quinone oxido-reductase (NQO1), and also by siRNA for NQO1, demonstrating that NQO1-induced bioreduction of β-lap is an essential step in β-lap-induced cell death. Irradiation of A549 cells with 4 Gy caused a long-lasting upregulation of NQO1, thereby increasing NQO1-mediated β-lap-induced cell deaths. Although the direct cause of β-lap-induced apoptosis is not yet clear, β-lap treatment reduced the expression of p53 and NF-κB, whereas it increased cytochrome C release, caspase-3 activity, and γH2AX foci formation. Importantly, β-lap treatment immediately after irradiation enhanced radiation-induced cell death, indicating that β-lap sensitizes cancer cells to radiation, in addition to directly killing some of the cells. The growth of A549 tumors induced in immunocompromised mice could be markedly suppressed by local radiation therapy when followed by β-lap treatment. This is the first study to demonstrate that combined radiotherapy and β-lap treatment can have a significant effect on human tumor xenografts. PMID:17786182

Dogs lap using acceleration-driven open pumping

PubMed Central

Gart, Sean; Socha, John J.; Vlachos, Pavlos P.; Jung, Sunghwan

2015-01-01

Dogs lap because they have incomplete cheeks and cannot suck. When lapping, a dog’s tongue pulls a liquid column from the bath, suggesting that the hydrodynamics of column formation are critical to understanding how dogs drink. We measured lapping in 19 dogs and used the results to generate a physical model of the tongue’s interaction with the air–fluid interface. These experiments help to explain how dogs exploit the fluid dynamics of the generated column. The results demonstrate that effects of acceleration govern lapping frequency, which suggests that dogs curl the tongue to create a larger liquid column. Comparing lapping in dogs and cats reveals that, despite similar morphology, these carnivores lap in different physical regimes: an unsteady inertial regime for dogs and steady inertial regime for cats. PMID:26668382

eIF2 kinases mediate β-lapachone toxicity in yeast and human cancer cells

PubMed Central

Menacho-Márquez, Mauricio; Rodríguez-Hernández, Carlos J; Villaronga, M Ángeles; Pérez-Valle, Jorge; Gadea, José; Belandia, Borja; Murguía, José R

2015-01-01

β-lapachone (β-lap) is a novel anticancer agent that selectively induces cell death in human cancer cells, by activation of the NQO1 NAD(P)H dehydrogenase and radical oxygen species (ROS) generation. We characterized the gene expression profile of budding yeast cells treated with β-lap using cDNA microarrays. Genes involved in tolerance to oxidative stress were differentially expressed in β-lap treated cells. β-lap treatment generated reactive oxygen species (ROS), which were efficiently blocked by dicoumarol, an inhibitor of NADH dehydrogenases. A yeast mutant in the mitocondrial NADH dehydrogenase Nde2p was found to be resistant to β-lap treatment, despite inducing ROS production in a WT manner. Most interestingly, DNA damage responses triggered by β-lap were abolished in the nde2Δ mutant. Amino acid biosynthesis genes were also induced in β-lap treated cells, suggesting that β-lap exposure somehow triggered the General Control of Nutrients (GCN) pathway. Accordingly, β-lap treatment increased phosphorylation of eIF2α subunit in a manner dependent on the Gcn2p kinase. eIF2α phosphorylation required Gcn1p, Gcn20p and Nde2p. Gcn2p was also required for cell survival upon exposure to β-lap and to elicit checkpoint responses. Remarkably, β-lap treatment increased phosphorylation of eIF2α in breast tumor cells, in a manner dependent on the Nde2p ortholog AIF, and the eIF2 kinase PERK. These findings uncover a new target pathway of β-lap in yeast and human cells and highlight a previously unknown functional connection between Nde2p, Gcn2p and DNA damage responses. PMID:25590579

Preparation of Laponite Bioceramics for Potential Bone Tissue Engineering Applications

PubMed Central

Li, Kai; Ju, Yaping; Li, Jipeng; Zhang, Yongxing; Li, Jinhua; Liu, Xuanyong; Shi, Xiangyang; Zhao, Qinghua

2014-01-01

We report a facile approach to preparing laponite (LAP) bioceramics via sintering LAP powder compacts for bone tissue engineering applications. The sintering behavior and mechanical properties of LAP compacts under different temperatures, heating rates, and soaking times were investigated. We show that LAP bioceramic with a smooth and porous surface can be formed at 800°C with a heating rate of 5°C/h for 6 h under air. The formed LAP bioceramic was systematically characterized via different methods. Our results reveal that the LAP bioceramic possesses an excellent surface hydrophilicity and serum absorption capacity, and good cytocompatibility and hemocompatibility as demonstrated by resazurin reduction assay of rat mesenchymal stem cells (rMSCs) and hemolytic assay of pig red blood cells, respectively. The potential bone tissue engineering applicability of LAP bioceramic was explored by studying the surface mineralization behavior via soaking in simulated body fluid (SBF), as well as the surface cellular response of rMSCs. Our results suggest that LAP bioceramic is able to induce hydroxyapatite deposition on its surface when soaked in SBF and rMSCs can proliferate well on the LAP bioceramic surface. Most strikingly, alkaline phosphatase activity together with alizarin red staining results reveal that the produced LAP bioceramic is able to induce osteoblast differentiation of rMSCs in growth medium without any inducing factors. Finally, in vivo animal implantation, acute systemic toxicity test and hematoxylin and eosin (H&E)-staining data demonstrate that the prepared LAP bioceramic displays an excellent biosafety and is able to heal the bone defect. Findings from this study suggest that the developed LAP bioceramic holds a great promise for treating bone defects in bone tissue engineering. PMID:24955961

Lysosome-oriented, dual-stage pH-responsive polymeric micelles for β-Lapachone delivery.

PubMed

Zhou, Yinjian; Dong, Ying; Huang, Gang; Wang, Yiguang; Huang, Xiaonan; Zhang, Fayun; Boothman, David A; Gao, Jinming; Liang, Wei

2016-12-14

β-Lapachone (β-lap), a novel anticancer agent, is bioactivated by NADP(H):quinone oxidoreductase 1 (NQO1), an enzyme over-expressed in numerous tumors, including lung, pancreas, breast, and prostate cancers. Fast renal clearance and methemaglobinemia / hemolytic side-effects from the clinical formulation (β-lap-hydroxyl propyl-β-cyclodextrin complex) hindered its clinical translation. Here, we investigated a dual model pH responsive polymers for β-lap delivery. Three pH-sensitive linkages, including acylhydrazone, ketal and imine bonds for β-lap prodrug syntheses result in an aryl imine linkage the most optimal linkage. The conversion to β-lap was 2.8%, 4.5% and 100% at pH 7.4, 6.5 and 5.0 in 8 h, respectively. β-lap aryl imine prodrug conjugated ultra pH-sensitive (UPS) polymer reached high β-lap loading density (8.3%) and exhibited dual-stages responsiveness to pH variation. In pHs under pH t , at stage I, micelle immediately dissociation and subsequently entering stage II, micelles start quickly release β-lap. In vitro release study showed that the micelles constantly release β-lap (14.9 ± 0.1%) at pHs above pH t in 72 h, whereas boosted release of β-lap (79.4 ± 1.2%) at pH 5.0. Micelle intracellular distribution predominantly in the lysosome organelle guaranteed their pH responsive dissociation and subsequently β-lap controlled release. The M-P micelles retained NQO1-dependent cytotoxicity in A549 lung cancer cells, similar to free drug in both efficacy and mechanism of cell death. The lysosome-oriented dual-stage ultra pH responsive β-lap prodrug micelles potentially offer an alternative nanotherapeutic strategy for lung, as well as other NQO1+ cancer therapies.

Intestinal Permeability of β-Lapachone and Its Cyclodextrin Complexes and Physical Mixtures.

PubMed

Mangas-Sanjuan, Victor; Gutiérrez-Nieto, Jorge; Echezarreta-López, Magdalena; González-Álvarez, Isabel; González-Álvarez, Marta; Casabó, Vicente-Germán; Bermejo, Marival; Landin, Mariana

2016-12-01

β-Lapachone (βLAP) is a promising, poorly soluble, antitumoral drug. βLAP combination with cyclodextrins (CDs) improves its solubility and dissolution but there is not enough information about the impact of cyclodextrins on βLAP intestinal permeability. The objectives of this work were to characterize βLAP intestinal permeability and to elucidate cyclodextrins effect on the dissolution properties and on the intestinal permeability. The final goal was to evaluate CDs influence on the oral absorption of βLAP. Binary systems (physical mixtures and inclusion complexes) including βLAP and CDs (β-cyclodextrin: βCD, random-methyl-β-cyclodextrin: RMβCD and sulfobutylether-β-cyclodextrin: SBEβCD) have been prepared and analysed by differential scanning calorimetry. βLAP (and its combinations with CDs) absorption rate coefficients and effective permeability values have been determined in vitro in MDCK or MDCK-Mdr1 monolayers and in situ in rat by a closed loop perfusion technique. DSC results confirmed the formation of the inclusion complexes. βLAP-CDs inclusion complexes improve drug solubility and dissolution rate in comparison with physical mixtures. βLAP presented a high permeability value which can provide complete oral absorption. Its oral absorption is limited by its low solubility and dissolution rate. Cyclodextrin (both as physical mixtures and inclusion complexes) showed a positive effect on the intestinal permeability of βLAP. Complexation with CDs does not reduce βLAP intestinal permeability in spite of the potential negative effect of the reduction in free fraction of the drug. The use of RMβCD or SBEβCD inclusion complexes could benefit βLAP oral absorption by enhancing its solubility, dissolution rate and permeability.

Listeria monocytogenes uses Listeria adhesion protein (LAP) to promote bacterial transepithelial translocation and induces expression of LAP receptor Hsp60.

PubMed

Burkholder, Kristin M; Bhunia, Arun K

2010-12-01

Listeria monocytogenes interaction with the intestinal epithelium is a key step in the infection process. We demonstrated that Listeria adhesion protein (LAP) promotes adhesion to intestinal epithelial cells and facilitates extraintestinal dissemination in vivo. The LAP receptor is a stress response protein, Hsp60, but the precise role for the LAP-Hsp60 interaction during Listeria infection is unknown. Here we investigated the influence of physiological stressors and Listeria infection on host Hsp60 expression and LAP-mediated bacterial adhesion, invasion, and transepithelial translocation in an enterocyte-like Caco-2 cell model. Stressors such as heat (41°C), tumor necrosis factor alpha (TNF-α) (100 U), and L. monocytogenes infection (10(4) to 10(6) CFU/ml) significantly (P < 0.05) increased plasma membrane and intracellular Hsp60 levels in Caco-2 cells and consequently enhanced LAP-mediated L. monocytogenes adhesion but not invasion of Caco-2 cells. In transepithelial translocation experiments, the wild type (WT) exhibited 2.7-fold more translocation through Caco-2 monolayers than a lap mutant, suggesting that LAP is involved in transepithelial translocation, potentially via a paracellular route. Short hairpin RNA (shRNA) suppression of Hsp60 in Caco-2 cells reduced WT adhesion and translocation 4.5- and 3-fold, respectively, while adhesion remained unchanged for the lap mutant. Conversely, overexpression of Hsp60 in Caco-2 cells enhanced WT adhesion and transepithelial translocation, but not those of the lap mutant. Furthermore, initial infection with a low dosage (10(6) CFU/ml) of L. monocytogenes increased plasma membrane and intracellular expression of Hsp60 significantly, which rendered Caco-2 cells more susceptible to subsequent LAP-mediated adhesion and translocation. These data provide insight into the role of LAP as a virulence factor during intestinal epithelial infection and pose new questions regarding the dynamics between the host stress response and pathogen infection.

LAP degradation product reflects plasma kallikrein-dependent TGF-β activation in patients with hepatic fibrosis.

PubMed

Hara, Mitsuko; Kirita, Akiko; Kondo, Wakako; Matsuura, Tomokazu; Nagatsuma, Keisuke; Dohmae, Naoshi; Ogawa, Shinji; Imajoh-Ohmi, Shinobu; Friedman, Scott L; Rifkin, Daniel B; Kojima, Soichi

2014-01-01

Byproducts of cytokine activation are sometimes useful as surrogate biomarkers for monitoring cytokine generation in patients. Transforming growth factor (TGF)-β plays a pivotal role in pathogenesis of hepatic fibrosis. TGF-β is produced as part of an inactive latent complex, in which the cytokine is trapped by its propeptide, the latency-associated protein (LAP). Therefore, to exert its biological activity, TGF-β must be released from the latent complex. Several proteases activate latent TGF-β by cutting LAP. We previously reported that Camostat Mesilate, a broad spectrum protease inhibitor, which is especially potent at inhibiting plasma kallikrein (PLK), prevented liver fibrosis in the porcine serum-induced liver fibrosis model in rats. We suggested that PLK may work as an activator of latent TGF-β during the pathogenesis of liver diseases in the animal models. However, it remained to be elucidated whether this activation mechanism also functions in fibrotic liver in patients. Here, we report that PLK cleaves LAP between R(58) and L(59) residues. We have produced monoclonal antibodies against two degradation products of LAP (LAP-DP) by PLK, and we have used these specific antibodies to immunostain LAP-DP in liver tissues from both fibrotic animals and patients. The N-terminal side LAP-DP ending at R(58) (R(58) LAP-DP) was detected in liver tissues, while the C-terminal side LAP-DP beginning at L(59) (L(59) LAP-DP) was not detectable. The R(58) LAP-DP was seen mostly in α-smooth muscle actin-positive activated stellate cells. These data suggest for the first time that the occurrence of a PLK-dependent TGF-β activation reaction in patients and indicates that the LAP-DP may be useful as a surrogate marker reflecting PLK-dependent TGF-β activation in fibrotic liver both in animal models and in patients.

Thermomechanical behavior of tin-rich (lead-free) solders

NASA Astrophysics Data System (ADS)

Sidhu, Rajen Singh

In order to adequately characterize the behavior of ball-grid-array (BGA) Pb-free solder spheres in electronic devices, the microstructure and thermomechanical behavior need to be studied. Microstructure characterization of pure Sn, Sn-0.7Cu, Sn-3.5Ag, and Sn-3.9Ag-0.7Cu alloys was conducted using optical microscopy, scanning electron microscopy, transmission electron microscopy, image analysis, and a novel serial sectioning 3D reconstruction process. Microstructure-based finite-element method (FEM) modeling of deformation in Sn-3.5Ag alloy was conducted, and it will be shown that this technique is more accurate when compared to traditional unit cell models for simulating and understanding material behavior. The effect of cooling rate on microstructure and creep behavior of bulk Sn-rich solders was studied. The creep behavior was evaluated at 25, 95, and 120°C. Faster cooling rates were found to increase the creep strength of the solders due to refinement of the solder microstructure. The creep behavior of Sn-rich single solder spheres reflowed on Cu substrates was studied at 25, 60, 95, and 130°C. Testing was conducted using a microforce testing system, with lap-shear geometry samples. The solder joints displayed two distinct creep behaviors: (a) precipitation-strengthening (Sn-3.5Ag and Sn-3.9Ag-0.7Cu) and (b) power law creep accommodated by grain boundary sliding (GBS) (Sn and Sn-0.7Cu). The relationship between microstructural features (i.e. intermetallic particle size and spacing), stress exponents, threshold stress, and activation energies are discussed. The relationship between small-length scale creep behavior and bulk behavior is also addressed. To better understand the damage evolution in Sn-rich solder joints during thermal fatigue, the local damage will be correlated to the cyclic hysteresis behavior and crystal orientations present in the Sn phase of solder joints. FEM modeling will also be utilized to better understand the macroscopic and local strain response of the lap shear geometry.

Study of two different thin film coating methods in transmission laser micro-joining of thin Ti-film coated glass and polyimide for biomedical applications.

PubMed

Sultana, T; Georgiev, G L; Baird, R J; Auner, G W; Newaz, G; Patwa, R; Herfurth, H J

2009-07-01

Biomedical devices and implants require precision joining for hermetic sealing which can be achieved with low power lasers. The effect of two different thin metal film coating methods was studied in transmission laser micro-joints of titanium-coated glass and polyimide. The coating methods were cathodic arc physical vapor deposition (CA-PVD) and electron beam evaporation (EB-PVD). Titanium-coated glass joined to polyimide film can have neural electrode application. The improvement of the joint quality will be essential for robust performance of the device. Low power fiber laser (wave length = 1100 nm) was used for transmission laser micro-joining of thin titanium (Ti) film (approximately 200 nm) coated Pyrex borosilicate 7740 glass wafer (0.5 mm thick) and polyimide (Imidex) film (0.2 mm thick). Ti film acts as the coupling agent in the joining process. The Ti film deposition rate in the CA-PVD was 5-10 A/s and in the EB-PVD 1.5 A/s. The laser joint strength was measured by a lap shear test, the Ti film surfaces were analyzed by atomic force microscopy (AFM) and the lap shear tested joints were analyzed by optical microscopy and scanning electron microscopy (SEM). The film properties and the failure modes of the joints were correlated to joint strength. The CA-PVD produced around 4 times stronger laser joints than EB-PVD. The adhesion of the Ti film on glass by CA-PVD is better than that of the EB-PVD method. This is likely to be due to a higher film deposition rate and consequently higher adhesion or sticking coefficient for the CA-PVD particles arriving on the substrate compared to that of the EB-PVD film. EB-PVD shows poor laser bonding properties due to the development of thermal hotspots which occurs from film decohesion.

Effects of light on responses to low atmospheric pressure stunning in broilers.

PubMed

Martin, J E; Christensen, K; Vizzier-Thaxton, Y; McKeegan, D E F

2016-10-01

Low atmospheric pressure stunning (LAPS) is a novel approach to poultry stunning involving the application of gradual decompression lasting 280 s according to a prescribed pressure curve. The aim of this study was to determine how behavioural, electroencephalogram (EEG) and electrocardiogram (ECG) responses to LAPS are influenced by illumination of the decompression chamber. A secondary aim was to examine responses to the decompression chamber without LAPS being applied, as such a "sham" control has been absent in previous studies. A two by two factorial design was employed, with LAPS/light, LAPS/dark, sham/light and sham/dark treatments (N = 20 per treatment). Broilers were exposed to each treatment in pairs, in each of which one bird was instrumented for recording EEG and ECG. Illumination was applied at 500 lx, and in sham treatments, birds were identically handled but remained undisturbed in the LAPS chamber without decompression for 280 s. Birds which underwent the sham treatment exhibited behaviours which were also observed in LAPS (e.g. sitting) while those exposed to LAPS exhibited hypoxia-related behaviours (e.g. ataxia, loss of posture). Behavioural latencies and durations were increased in the sham treatments, since the whole cycle time was available (in LAPS; birds were motionless by 186 s). Within the sham treatments, illumination increased active behaviour and darkness induced sleep, but slow-wave EEG was seen in both. The pattern of EEG response to LAPS (steep reduction in median frequency in the first 60 s and increased total power) was similar, irrespective of illumination, though birds in darkness had shorter latencies to loss of consciousness and isoelectric EEG. Cardiac responses to LAPS (pronounced bradycardia) closely matched those reported previously and were not affected by illumination. The effects of LAPS/sham treatment primarily reflected the presence/absence of hypoxia, while illumination affected activity/sleep levels in sham-treated birds and slowed time to unconsciousness in birds undergoing LAPS. Therefore, it is recommended that LAPS be conducted in darkness for poultry.

Molecular cloning and characterization of leucine aminopeptidase gene from Taenia pisiformis.

PubMed

Zhang, Shaohua; Cai, Xuepeng; Luo, Xuenong; Wang, Shuai; Guo, Aijiang; Hou, Junling; Wu, Run

2018-03-01

Leucine aminopeptidase (LAP, EC: 3.4.11.1) is an important metalloexopeptidase that catalyze the hydrolysis of amino-terminal leucine residues from polypeptides and proteins. In this study, a full length of cDNA encoding leucine aminopeptidase of Taenia pisiformis (TpLAP) was cloned by rapid amplification of cDNA-ends using the polymerase chain reaction (RACE-PCR) method. The full-length cDNA of the TpLAP gene is 1823 bp and contains a 1569 bp ORF encoding 533 amino acids with a putative mass of 56.4 kDa. TpLAP contains two characteristic motifs of the M17LAP family in the C-terminal sequence: the metal binding site 265-[VGKG]-271 and the catalytic domain motif 351-[NTDAEGRL]-357. The soluble GST-TpLAP protein was expressed in Escherichia coli Transetta (DE3) and four specific anti-TpLAP monoclonal antibodies (mAbs) were prepared. In enzymatic assays, the optimal activity was observed at pH 9.5 at 45 °C. GST-TpLAP displayed a hydrolyzing activity for the Leu-pNA substrate with a maximum activity of 46 U/ml. The enzymatic activity was significantly enhanced by Mn 2+ and completely inhibited by 20 nM bestatin and 0.15 mM EDTA. The native TpLAP was detected specifically in ES components of adult T. pisiformis by western blotting using anti-TpLAP mAb as a probe. Quantitative real-time PCR revealed that the TpLAP gene was expressed at a high level in adult worm tissues, especially in the gravid proglottids (50.71-fold). Immunolocalization analysis showed that TpLAP was located primarily in the subtegumental parenchyma zone and the uterine wall of adult worms. Our results indicate that TpLAP is a new member of the M17LAP family and can be considered as a stage-differentially expressed protein. These findings might provide new insights into the study of the mechanisms of growth, development and survival of T. pisiformis in the final host and have potential value as an attractive target for drug therapy or vaccine intervention. Copyright © 2018 Elsevier Inc. All rights reserved.

An N-terminal Retention Module Anchors the Giant Adhesin LapA of Pseudomonas fluorescens at the Cell Surface: A Novel Sub-family of Type I Secretion Systems.

PubMed

Smith, T Jarrod; Font, Maria E; Kelly, Carolyn M; Sondermann, Holger; O'Toole, George A

2018-02-05

LapA of Pseudomonas fluorescens Pf0-1 belongs to a diverse family of cell surface associated bacterial adhesins that are secreted via the type-1 secretion system (T1SS). We previously reported that the periplasmic protease LapG cleaves the N-terminus of LapA at a canonical dialanine motif to release the adhesin from the cell surface under conditions unfavorable to biofilm formation, thus decreasing biofilm formation. Here, we characterize LapA as the first type 1 secreted substrate that does not follow the "one-step" rule of T1SS. Rather, a novel N-terminal element, called the retention module (RM), localizes LapA at the cell surface as a secretion intermediate. Our genetic, biochemical, and molecular modeling analysis support a model wherein LapA is tethered to the cell surface through its T1SS outer membrane TolC-like pore, LapE, until LapG cleaves LapA in the periplasm. We further demonstrate this unusual retention strategy is likely conserved among LapA-like proteins, and reveals a new subclass of T1SS ABC transporters involved in transporting this group of surface-associated, LapA-like adhesins. These studies demonstrate a novel cell surface retention strategy used throughout the Proteobacteria and highlight a previously unappreciated flexibility of function for T1SS. Importance. Bacteria have evolved multiple secretion strategies to interact with their environment. For many bacteria, the secretion of cell surface associated adhesins is key for initiating contact with a preferred substratum to facilitate biofilm formation. Our work demonstrates that P. fluorescens uses a previously unrecognized secretion strategy to retain the giant adhesin LapA at its cell surface. Further, we identify likely LapA-like adhesins in various pathogenic and commensal Proteobacteria and provide phylogenetic evidence that these adhesins are secreted by a new subclass of T1SS ABC transporters. Copyright © 2018 American Society for Microbiology.

Investigation on the Effect of Pulsed Energy on Strength of Fillet Lap Laser Welded AZ31B Magnesium Alloys

NASA Astrophysics Data System (ADS)

Salleh, M. N. M.; Ishak, M.; Aiman, M. H.; Idris, S. R. A.; Romlay, F. R. M.

2017-09-01

AZ31B magnesium alloy have been hugely applied in the aerospace, automotive, and electronic industries. However, welding thin sheet AZ31B was challenging due to its properties which is easily to evaporated especially using conventional fusion welding method such as metal inert gas (MIG). Laser could be applied to weld this metal since it produces lower heat input. The application of fiber laser welding has been widely since this type of laser could produce better welding product especially in the automotive sectors. Low power fiber laser was used to weld this non-ferrous metal where pulse wave (PW) mode was used. Double fillet lap joint was applied to weld as thin as 0.6 mm thick of AZ31B and the effect of pulsed energy on the strength was studied. Bond width, throat length, and penetration depth also was studied related to the pulsed energy which effecting the joint. Higher pulsed energy contributes to the higher fracture load with angle of irradiation lower than 3 °

Shear fatigue crack growth - A literature survey

NASA Technical Reports Server (NTRS)

Liu, H. W.

1985-01-01

Recent studies of shear crack growth are reviewed, emphasizing test methods and data analyses. The combined mode I and mode II elastic crack tip stress fields are considered. The development and design of the compact shear specimen are described, and the results of fatigue crack growth tests using compact shear specimens are reviewed. The fatigue crack growth tests are discussed and the results of inclined cracks in tensile panels, center cracks in plates under biaxial loading, cracked beam specimens with combined bending and shear loading, center-cracked panels and double edge-cracked plates under cyclic shear loading are examined and analyzed in detail.

Dynamic deformation measurement and analysis of active stressed lap using optical method

NASA Astrophysics Data System (ADS)

Zhang, Qican; Su, Xianyu; Liu, Yuankun; Xiang, Liqun

2007-12-01

The active stressed lap is the heart of polishing process. A novel non-contact optical method of dynamic deformation measurement and analysis of an active stressed lap is put forward. This method, based on structured illumination, is able to record full-field information of the bending and rotating stressed lap dynamically and continuously, while its profile is changed under computer control, and restore the whole process of lap deformation varied with time at different position and rotating angle. It has been verified by experiments that this proposed method will be helpful to the opticians to ensure the stressed lap as expected.

Notebook computer use on a desk, lap and lap support: effects on posture, performance and comfort.

PubMed

Asundi, Krishna; Odell, Dan; Luce, Adam; Dennerlein, Jack T

2010-01-01

This study quantified postures of users working on a notebook computer situated in their lap and tested the effect of using a device designed to increase the height of the notebook when placed on the lap. A motion analysis system measured head, neck and upper extremity postures of 15 adults as they worked on a notebook computer placed on a desk (DESK), the lap (LAP) and a commercially available lapdesk (LAPDESK). Compared with the DESK, the LAP increased downwards head tilt 6 degrees and wrist extension 8 degrees . Shoulder flexion and ulnar deviation decreased 13 degrees and 9 degrees , respectively. Compared with the LAP, the LAPDESK decreased downwards head tilt 4 degrees , neck flexion 2 degrees , and wrist extension 9 degrees. Users reported less discomfort and difficulty in the DESK configuration. Use of the lapdesk improved postures compared with the lap; however, all configurations resulted in high values of wrist extension, wrist deviation and downwards head tilt. STATEMENT OF RELEVANCE: This study quantifies postures of users working with a notebook computer in typical portable configurations. A better understanding of the postures assumed during notebook computer use can improve usage guidelines to reduce the risk of musculoskeletal injuries.

Daily rhythms of left atrial pressure in beagle dogs with mitral valve regurgitation.

PubMed

Ishikawa, T; Tanaka, R; Suzuki, S; Saida, Y; Soda, A; Fukushima, R; Yamane, Y

2009-01-01

Mitral valve regurgitation (MR) causes increased left atrial pressure (LAP) and is associated with occurrence of clinical signs. It will be useful to understand diurnal variations of LAP for the management of MR. Circulatory parameters and diurnal rhythm are linked to clinical signs in cardiac diseases. LAP also exhibits a diurnal rhythm in dogs with MR. Five healthy Beagle dogs weighing 9.8-12.8 kg (3 males and 2 females; aged 2 years) were used. A radiotelemetry system for continuous measurement of LAP was used in this study. Rupture of the chordae tendineae was experimentally induced via left atriotomy, and a transmitter catheter was inserted into the left atrium. The body of the transmitter was implanted SC. After clinical condition was stabilized, the severity of MR was evaluated by echocardiography, and LAP was recorded for 72 consecutive hours for the analysis of diurnal variation. Abrupt increases in LAP, which averaged 16.7 mmHg, were observed at feeding periods. In contrast, strong diurnal LAP variations were found, with a significant but slight increase in daytime LAP compared with nighttime LAP. Diurnal LAP is characterized by a slight but significant nocturnal decrease and abrupt increases in response to excitation. The latter seemed to be more important considering the relationship with clinical manifestations. The clinical relevance of exercise restriction in the management of MR was acknowledged.

Expression of Surface Protein LapB by a Wide Spectrum of Listeria monocytogenes Serotypes as Demonstrated with Anti-LapB Monoclonal Antibodies

PubMed Central

Boivin, Teela; Elmgren, Cathie; Brooks, Brian W.; Huang, Hongsheng; Pagotto, Franco

2016-01-01

ABSTRACT Protein antigens expressed on the surface of all strains of Listeria monocytogenes and absent from nonpathogenic Listeria spp. are presumably useful targets for pathogen identification, detection, and isolation using specific antibodies (Abs). To seek such surface proteins expressed in various strains of L. monocytogenes for diagnostic applications, we focused on a set of surface proteins known to be involved or putatively involved in L. monocytogenes virulence and identified Listeria adhesion protein B (LapB) as a candidate based on the bioinformatics analysis of whole-genome sequences showing that the gene coding for LapB was present in L. monocytogenes strains and absent from strains of other Listeria spp. Immunofluorescence microscopy (IFM), performed with rabbit polyclonal antibodies against the recombinant LapB protein (rLapB) of L. monocytogenes serotype 4b strain L10521, confirmed expression of LapB on the surface. A panel of 48 mouse monoclonal antibodies (MAbs) to rLaB was generated, and 7 of them bound strongly to the surface of L. monocytogenes cells as demonstrated using IFM. Further characterization of these 7 anti-LapB MAbs, using an enzyme-linked immunosorbent assay (ELISA), revealed that 6 anti-LapB MAbs (M3484, M3495, M3500, M3509, M3517, and M3519) reacted strongly with 46 (86.8%) of 53 strains representing 10 of the 12 serotypes tested (1/2a, 1/2b, 1/2c, 3a, 3b, 3c, 4ab, 4b, 4d, and 4e). These results indicate that LapB, together with companion anti-LapB MAbs, can be targeted as a biomarker for the detection and isolation of various L. monocytogenes strains from contaminated foods. IMPORTANCE Strains of L. monocytogenes are traditionally grouped into serotypes. Identification of a surface protein expressed in all or the majority of at least 12 serotypes would aid in the development of surface-binding monoclonal antibodies (MAbs) for detection and isolation of L. monocytogenes from foods. Bioinformatics analysis revealed that the gene coding for Listeria adhesion protein B (LapB), a surface protein involved in L. monocytogenes virulence, was present in L. monocytogenes strains and absent from other Listeria spp. Polyclonal antibodies against recombinant LapB (rLapB) detected the exposed epitopes on the surface of L. monocytogenes. Production and extensive assessment of 48 MAbs to rLapB showed that 6 anti-LapB MAbs (M3484, M3495, M3500, M3509, M3517, and M3519) detected the expression of LapB in a wide range of L. monocytogenes isolates representing 10 of 12 serotypes tested, suggesting that LapB, together with specific MAbs, can be targeted as a biomarker for pathogen detection and isolation. PMID:27613687

Expression of Surface Protein LapB by a Wide Spectrum of Listeria monocytogenes Serotypes as Demonstrated with Anti-LapB Monoclonal Antibodies.

PubMed

Boivin, Teela; Elmgren, Cathie; Brooks, Brian W; Huang, Hongsheng; Pagotto, Franco; Lin, Min

2016-11-15

Protein antigens expressed on the surface of all strains of Listeria monocytogenes and absent from nonpathogenic Listeria spp. are presumably useful targets for pathogen identification, detection, and isolation using specific antibodies (Abs). To seek such surface proteins expressed in various strains of L. monocytogenes for diagnostic applications, we focused on a set of surface proteins known to be involved or putatively involved in L. monocytogenes virulence and identified Listeria adhesion protein B (LapB) as a candidate based on the bioinformatics analysis of whole-genome sequences showing that the gene coding for LapB was present in L. monocytogenes strains and absent from strains of other Listeria spp. Immunofluorescence microscopy (IFM), performed with rabbit polyclonal antibodies against the recombinant LapB protein (rLapB) of L. monocytogenes serotype 4b strain L10521, confirmed expression of LapB on the surface. A panel of 48 mouse monoclonal antibodies (MAbs) to rLaB was generated, and 7 of them bound strongly to the surface of L. monocytogenes cells as demonstrated using IFM. Further characterization of these 7 anti-LapB MAbs, using an enzyme-linked immunosorbent assay (ELISA), revealed that 6 anti-LapB MAbs (M3484, M3495, M3500, M3509, M3517, and M3519) reacted strongly with 46 (86.8%) of 53 strains representing 10 of the 12 serotypes tested (1/2a, 1/2b, 1/2c, 3a, 3b, 3c, 4ab, 4b, 4d, and 4e). These results indicate that LapB, together with companion anti-LapB MAbs, can be targeted as a biomarker for the detection and isolation of various L. monocytogenes strains from contaminated foods. Strains of L. monocytogenes are traditionally grouped into serotypes. Identification of a surface protein expressed in all or the majority of at least 12 serotypes would aid in the development of surface-binding monoclonal antibodies (MAbs) for detection and isolation of L. monocytogenes from foods. Bioinformatics analysis revealed that the gene coding for Listeria adhesion protein B (LapB), a surface protein involved in L. monocytogenes virulence, was present in L. monocytogenes strains and absent from other Listeria spp. Polyclonal antibodies against recombinant LapB (rLapB) detected the exposed epitopes on the surface of L. monocytogenes Production and extensive assessment of 48 MAbs to rLapB showed that 6 anti-LapB MAbs (M3484, M3495, M3500, M3509, M3517, and M3519) detected the expression of LapB in a wide range of L. monocytogenes isolates representing 10 of 12 serotypes tested, suggesting that LapB, together with specific MAbs, can be targeted as a biomarker for pathogen detection and isolation. © Crown copyright 2016.

Influence of the resin on interlaminar mixed-mode fracture

NASA Technical Reports Server (NTRS)

Johnson, W. S.; Mangalgiri, P. D.

1987-01-01

Both literature review data and new data on toughness behavior of seven matrix and adhesive systems in four types of tests were studied in order to assess the influence of the resin on interlaminar fracture. Mixed mode (i.e., various combinations of opening mode 1, G sub 1, and shearing mode 2; G sub 2) fracture toughness data showed that the mixed mode relationship for failure appears to be linear in terms of G sub 1 and G sub 2. The study further indicates that fracture of brittle resins is controlled by the G sub 1 component, and that fracture of many tough resins is controlled by total strain-energy release rate, G sub T. Regarding the relation of polymer structure and the mixed mode fracture: high mode 1 toughness requires resin dilatation; dilatation is low in unmodified epoxies at room temperature/dry conditions; dilatation is higher in plasticized epoxies, heated epoxies, and in modified epoxies; modification improves mode 2 toughness only slightly compared with mode 1 improvements. Analytical aspects of the cracked lap shear test specimen were explored.

Interfacial crystalline structures in injection over-molded polypropylene and bond strength.

PubMed

Yan, Bowen; Wu, Hong; Jiang, Genjie; Guo, Shaoyun; Huang, Jian

2010-11-01

This paper describes interfacial crystalline structures found in injection overmolded polypropylene components and the relationship of these structures to bond strength between the components. The combined effects of the development of hierarchical gradient structures and the particular thermomechanical environment near the interface on the interfacial crystalline structures were investigated in detail by PLM, SEM, DSC, WAXD, and infrared dichroism spectroscopy. The experimental results showed that during molding there was competitive formation of interfacial crystalline structures consisted of "shish-kebab" layer (SKL) and a transcrystalline layers (TCL). Variation in shear stress (controlled by injection pressure and injection speed) plays an important role in the formation of the SKL. The formation of TCL is influenced by the thermal environment, namely melt temperature and mold temperature. Increasing within certain limits, interfacial temperature and the thermal gradient near the interface promotes β-iPP growth. The relationship between interfacial crystalline structures and interfacial bond strength was established by lap shear measurement. The interfacial bond strength is improved by enhancing the formation of TCL, but reduced if SKL predominates.

Influence of the resin on interlaminar mixed-mode fracture

NASA Technical Reports Server (NTRS)

Johnson, W. S.; Mangalgiri, P. D.

1985-01-01

Both literature review data and new data on toughness behavior of seven matrix and adhesive systems in four types of tests were studied in order to assess the influence of the resin on interlaminar fracture. Mixed mode (i.e., various combinations of opening mode 1, G sub 1, and shearing mode 2; G sub 2) fracture toughness data showed that the mixed mode relationship for failure appears to be linear in terms of G sub 1 and G sub 2. The study further indicates that fracture of brittle resins is controlled by the G sub 1 component, and that fracture of many tough resins is controlled by total strain-energy release rate, G sub T. Regarding the relation of polymer structure and the mixed mode fracture: high mode 1 toughness requires resin dilatation; dilatation is low in unmodified epoxies at room temperature/dry conditions; dilatation is higher in plasticized epoxies, heated epoxies, and in modified epoxies; modification improves mode 2 toughness only slightly compared with mode 1 improvements. Analytical aspects of the cracked lap shear test specimen were explored.

49 CFR 230.30 - Lap-joint seam boilers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Lap-joint seam boilers. 230.30 Section 230.30..., DEPARTMENT OF TRANSPORTATION STEAM LOCOMOTIVE INSPECTION AND MAINTENANCE STANDARDS Boilers and Appurtenances Inspection and Repair § 230.30 Lap-joint seam boilers. Every boiler having lap-joint longitudinal seams...

Auto Mechanics I. Learning Activity Packets (LAPs). Section D--Suspension.

ERIC Educational Resources Information Center

Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

This document contains six learning activity packets (LAPs) that outline the study activities for the "suspension" instructional area for an Auto Mechanics I course. The six LAPs cover the following topics: wheel bearings, tires and wheels, wheel balancing, suspension system, steering system, and wheel alignment. Each LAP contains a…

Standard Methods for Bolt-Bearing Testing of Textile Composites

NASA Technical Reports Server (NTRS)

Portanova, M. A.; Masters, J. E.

1995-01-01

The response of three 2-D braided materials to bolt bearing loading was evaluated using data generated by Boeing Defense and Space Group in Philadelphia, PA. Three test methods, stabilized single shear, unstabilized single shear, and double shear, were compared. In general, these textile composites were found to be sensitive to bolt bearing test methods. The stabilized single shear method yielded higher strengths than the unstabilized single shear method in all cases. The double shear test method always produced the highest strengths but these results may be somewhat misleading. It is therefore recommended that standard material comparisons be made using the stabilized single shear test method. The effects of two geometric parameters, W/D and e/D, were also studied. An evaluation of the effect of the specimen width (W) to hole diameter (D) ratio concluded that bolt bearing responses were consistent with open hole tension results. A W/D ratio of 6 or greater should be maintained. The proximity of the hole to the specimen edge significantly affected strength. In all cases, strength was improved by increasing the ratio of the distance from the hole center to the specimen edge (e) to the hole diameter (D) above 2. An e/D ratio of 3 or greater is recommended.

Apparatus and method for deterministic control of surface figure during full aperture polishing

DOEpatents

Suratwala, Tayyab Ishaq; Feit, Michael Dennis; Steele, William Augustus

2013-11-19

A polishing system configured to polish a lap includes a lap configured to contact a workpiece for polishing the workpiece; and a septum configured to contact the lap. The septum has an aperture formed therein. The radius of the aperture and radius the workpiece are substantially the same. The aperture and the workpiece have centers disposed at substantially the same radial distance from a center of the lap. The aperture is disposed along a first radial direction from the center of the lap, and the workpiece is disposed along a second radial direction from the center of the lap. The first and second radial directions may be opposite directions.

The Effect of Surface Irregularities on Wing Drag. II - Lap Joints. 2; Lap Joints

NASA Technical Reports Server (NTRS)

Hood, Manley J.

1938-01-01

Tests have been made in the NACA 8-foot high-speed wind tunnel of the drag caused by four types of lap joint. The tests were made on an airfoil of NACA 23012 section and 5-foot chord and covered in a range of speeds from 80 to 500 miles per hour and lift coefficients from 0 to 0.30. The increases in profile drag caused by representative arrangements of laps varied from 4 to 9%. When there were protruding rivet heads on the surface, the addition of laps increased the drag only slightly. Laps on the forward part of a wing increased the drag considerably more than those farther back.

Temperature, Pulse, and Respiration. Instructor's Packet. Learning Activity Package.

ERIC Educational Resources Information Center

Runge, Lillian

This instructor's packet accompanies the learning activity package (LAP) on temperature, pulse, and respiration. Contents included in the packet are a time sheet, suggested uses for the LAP, an instruction sheet, final LAP reviews, a final LAP review answer key, suggested activities, an additional resources list, and student completion cards to…

Auto Mechanics I. Learning Activity Packets (LAPs). Section C--Engine.

ERIC Educational Resources Information Center

Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

This document contains five learning activity packets (LAPs) that outline the study activities for the "engine" instructional area for an Auto Mechanics I course. The five LAPs cover the following topics: basic engine principles, cooling system, engine lubrication system, exhaust system, and fuel system. Each LAP contains a cover sheet…

Analysis and optimization of surface profile correcting mechanism of the pitch lap in large-aperture annular polishing

NASA Astrophysics Data System (ADS)

Zhang, Huifang; Yang, Minghong; Xu, Xueke; Wu, Lunzhe; Yang, Weiguang; Shao, Jianda

2017-10-01

The surface figure control of the conventional annular polishing system is realized ordinarily by the interaction between the conditioner and the lap. The surface profile of the pitch lap corrected by the marble conditioner has been measured and analyzed as a function of kinematics, loading conditions, and polishing time. The surface profile measuring equipment of the large lap based on laser alignment was developed with the accuracy of about 1μm. The conditioning mechanism of the conditioner is simply determined by the kinematics and fully fitting principle, but the unexpected surface profile deviation of the lap emerged frequently due to numerous influencing factors including the geometrical relationship, the pressure distribution at the conditioner/lap interface. Both factors are quantitatively evaluated and described, and have been combined to develop a spatial and temporal model to simulate the surface profile evolution of pitch lap. The simulations are consistent with the experiments. This study is an important step toward deterministic full-aperture annular polishing, providing a beneficial guidance for the surface profile correction of the pitch lap.

Outcome of laparoscopic ovariectomy and laparoscopic-assisted ovariohysterectomy in dogs: 278 cases (2003-2013).

PubMed

Corriveau, Kayla M; Giuffrida, Michelle A; Mayhew, Philipp D; Runge, Jeffrey J

2017-08-15

OBJECTIVE To compare outcomes for laparoscopic ovariectomy (LapOVE) and laparoscopic-assisted ovariohysterectomy (LapOVH) in dogs. DESIGN Retrospective case series. ANIMALS 278 female dogs. PROCEDURES Medical records of female dogs that underwent laparoscopic sterilization between 2003 and 2013 were reviewed. History, signalment, results of physical examination, results of preoperative diagnostic testing, details of the surgical procedure, durations of anesthesia and surgery, intraoperative and immediate postoperative (ie, during hospitalization) complications, and short- (≤ 14 days after surgery) and long-term (> 14 days after surgery) outcomes were recorded. Data for patients undergoing LapOVE versus LapOVH were compared. RESULTS Intraoperative and immediate postoperative complications were infrequent, and incidence did not differ between groups. Duration of surgery for LapOVE was significantly less than that for LapOVH; however, potential confounders were not assessed. Surgical site infection was identified in 3 of 224 (1.3%) dogs. At the time of long-term follow-up, postoperative urinary incontinence was reported in 7 of 125 (5.6%) dogs that underwent LapOVE and 12 of 82 (14.6%) dogs that underwent LapOVH. None of the dogs had reportedly developed estrus or pyometra by the time of final follow-up. Overall, 205 of 207 (99%) owners were satisfied with the surgery, and 196 of 207 (95%) would consider laparoscopic sterilization for their dogs in the future. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that short- and long-term outcomes were similar for female dogs undergoing sterilization by means of LapOVE or LapOVH; however, surgery time may have been shorter for dogs that underwent LapOVE. Most owners were satisfied with the outcome of laparoscopic sterilization.

A novel addition polyimide adhesive

NASA Technical Reports Server (NTRS)

St.clair, T. L.; Progar, D. J.

1981-01-01

An addition polyimide adhesive, LARC 13, was developed which shows promise for bonding both titanium and composites for applications which require service temperatures in excess of 533 K. The LARC 13 is based on an oligomeric bis nadimide containing a meta linked aromatic diamine. The adhesive melts prior to polymerization due to its oligomeric nature, thereby allowing it to be processed at 344 kPa or less. Therefore, LARC 13 is ideal for the bonding of honeycomb sandwich structures. After melting, the resin thermosets during the cure of the nadic endcaps to a highly crosslinked system. Few volatiles are evolved, thus allowing large enclosed structures to be bonded. Preparation of the adhesive as well as bonding, aging, and testing of lap shear and honeycomb samples are discussed.

An investigation of adhesive/adherend and fiber matrix interactions. [Ti 6-4 surfaces

NASA Technical Reports Server (NTRS)

Beck, B.; Siriwardane, R.; Wightman, J. P.

1981-01-01

Research during the report period focused on continued scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) of lap shear samples and flatwise tensile specimens and on the surface characterization of TiO2, Ti 6-4, and Ti powders with particular emphasis on their interaction with primer solutions of both polyphenylquinoxaline and LaRC-13 polyimide. The use of SEM and XPS in the analysis of Ti 6-4 adherend surfaces is described as well as differences in Ti 6-4 surface composition after different chemical pretreatments. Analysis of fractured surfaces is used to established the failure mode. The surface acidity of Ti 6-4 coupons can be established by reflectance visible spectroscopy using indicator dyes.

LARC-13 adhesive development

NASA Technical Reports Server (NTRS)

Hill, S. G.; Sheppard, C. H.; Johnson, J. C.

1980-01-01

A LARC-13 type adhesive system was developed and property data obtained that demonstrated improved thermomechanical properties superior to base LARC-13 adhesive. An improved adhesive for 589 K (600 F) use was developed by physical or chemical modification of LARC-13. The adhesive was optimized for titanium and composite bonding, and a compatible surface preparation for titanium and composite substrates was identified. The data obtained with the improved adhesive system indicated it would meet the 589 K (600 F) properties desired for application on space shuttle components. Average titanium lap shear data were: (1) 21.1 MPa (3355 psi) at RT, (2) 13.0 MPa (1881 psi) at 600 F, and (3) 16.4 MPa (2335) after aging 125 hours at 600 F and tested at 600 F.

Micromechanical and in situ shear testing of Al–SiC nanolaminate composites in a transmission electron microscope (TEM)

DOE PAGES

Mayer, Carl; Li, Nan; Mara, Nathan Allan; ...

2014-11-07

Nanolaminate composites show promise as high strength and toughness materials. Still, due to the limited volume of these materials, micron scale mechanical testing methods must be used to determine the properties of these films. To this end, a novel approach combining a double notch shear testing geometry and compression with a flat punch in a nanoindenter was developed to determine the mechanical properties of these films under shear loading. To further elucidate the failure mechanisms under shear loading, in situ TEM experiments were performed using a double notch geometry cut into the TEM foil. Aluminum layer thicknesses of 50nm andmore » 100nm were used to show the effect of constraint on the deformation. Higher shear strength was observed in the 50 nm sample (690±54 MPa) compared to the 100 nm sample (423±28.7 MPa). Additionally, failure occurred along the Al-SiC interface in the 50 nm sample as opposed to failure within the Al layer in the 100 nm sample.« less

Effect of simple shear flow on photosynthesis rate and morphology of micro algae

NASA Astrophysics Data System (ADS)

Mitsuhashi, S.; Fujimoto, M.; Muramatsu, H.; Tanishita, K.

The convective motion of micro algal suspension gives an advantageous effect on the photosynthetic rate in the bioreactor, however, the nature of convective effect on the photosynthesis has not been fully understood. The propose of this study concerns the nature of photosynthetic rate in a well-defined hydrodynamic shear flow of Spirulina platensis suspension, generated in a double rotating coaxial cylinders. The double rotating coaxial cylinders was installed in the incubator chamber with the controlled illumination intensity and temperature. Two kind of experiments, short and long term experiments, were performed to evaluate the direct effect of shear flow on the photosynthetic rate. The short term experiment indicates that the simple shear flow enables to augment the photosynthesis of Spirulina suspension and simultaneously causes the cell destruction due to the excessive shear stress. The long term experiment for 100 hours reveals that the growth rate and the morphology of Spirulina is sensitive to the external fluid mechanical stimulus. The long term application of mechanical stress on the algae may result in the adaptation of the photosynthetic function and morphology.

77 FR 21395 - Airworthiness Directives; Fokker Services B.V. Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-10

... would require performing a low frequency eddy current inspection for cracks of the lap joint of the rear...-frequency eddy current inspection of the lap joint for cracks and, depending on findings, repair of the lap... AD: Do a low frequency eddy current (LFEC) inspection for cracks of the lap joint of the rear...

New design deforming controlling system of the active stressed lap

NASA Astrophysics Data System (ADS)

Ying, Li; Wang, Daxing

2008-07-01

A 450mm diameter active stressed lap has been developed in NIAOT by 2003. We design a new lap in 2007. This paper puts on emphases on introducing the new deforming control system of the lap. Aiming at the control characteristic of the lap, a new kind of digital deforming controller is designed. The controller consists of 3 parts: computer signal disposing, motor driving and force sensor signal disposing. Intelligent numeral PID method is applied in the controller instead of traditional PID. In the end, the result of new deformation are given.

Apparatus and method for deterministic control of surface figure during full aperture pad polishing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suratwala, Tayyab Ishaq; Feit, Michael Douglas; Steele, William Augustus

A polishing system configured to polish a lap includes a lap configured to contact a workpiece for polishing the workpiece; and a septum configured to contact the lap. The septum has an aperture formed therein. The radius of the aperture and radius the workpiece are substantially the same. The aperture and the workpiece have centers disposed at substantially the same radial distance from a center of the lap. The aperture is disposed along a first radial direction from the center of the lap, and the workpiece is disposed along a second radial direction from the center of the lap. Themore » first and second radial directions may be opposite directions.« less

Cross-Shear in Metal-on-Polyethylene Articulation of Orthopaedic Implants and its Relationship to Wear

PubMed Central

Schwenke, T.; Wimmer, M. A.

2013-01-01

Wear of polyethylene (UHMWPE) is dependent on cross-shear. The aim of the present study was: 1) to develop a theoretical description of cross-shear, 2) to experimentally determine the relationship between cross-shear motion and UHMWPE wear using a wheel-on-flat apparatus, and 3) to calculate the work it takes to remove a unit volume of wear for the use in advanced computational models of wear. The theoretical description of cross-shear has been based on the previously reported finding that cross-shear is maximal when movement occurs perpendicular to fibril orientation. Here, cross-shear is described with a double-sinusoidal function that uses the angle between fibril orientation and velocity vector as input, and maximum cross-shear occurs at 90° and 270°. In the experimental part of the study, friction and wear of polyethylene were plotted against increasing sliding velocity vector angles, i.e. increasing cross-shear. It was found that wear intensified with increasing cross-shear, and wear depth could be predicted well using the double-sinusoidal function for cross-shear (r2=0.983). The friction data were then used to calculate the work to remove a unit particle by integrating the frictional force over the directional sliding distance. Using the wear volumes, determined for both longitudinal and perpendicular motion directions, the work to remove a unit volume of material was qy= 8.473 × 108 J/mm3 and qx= 1.321 × 108 J/mm3, respectively. Hence, 6.4 times more work was necessary to remove a unit wear volume in the direction of principal motion (i.e. along the molecular fibril orientation) than 90° perpendicular to it. In the future, these findings will be implemented in computational models to assess wear. PMID:23794761

Nd:YAG laser welding of coated sheet steel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Graham, M.P.; Kerr, H.W.; Weckman, D.C.

1994-12-31

Coated sheet steels are used extensively in the automotive industry for the fabrication of automobile body components; however, their reduced weldability by the traditional welding processes has led to numerous studies into the use of alternate process such as laser welding. In this paper, we present a modified joint geometry which allows high quality lap welds of coated sheet steels to be made by laser welding processes. Hot-dipped galvanized sheet (16 gauge), with a 60 g/m zinc coating was used in this study. A groove was created in the top sheet of a specimen pair by pressing piano wires ofmore » various diameters into the sheet. The specimens were clamped together in a lag-joint configuration such that they were in contacted only along the grove projection. A parametric study was conducted using the variables of welding speed, laser mean power (685 W, 1000 W and 1350 W), and grove size. Weld quality and weld pool dimensions were assessed using metallurgical cross-sections and image analysis techniques. Acceptable quality seam welds were produced in the galvanized sheet steel with both grove sizes when using 1000 W and 1350 W laser mean powers and a range of welding speeds. Results of the shear-tensile tests showed that high loads to failure, with failure occurring in the parent material, were predominately found in welds produced at speeds over 1.2 m/min and when using the high mean laser powers: 1000 W and 1350 W. A modified lap joint geometry, in which a groove is pre-placed in the top sheet of the lap-joint configuration, has been developed which permits laser welding of coated sheet steels. Good quality seam welds have been produced in 16 gauge galvanized sheet steels at speeds up to 2.7 m/min using a 2 kW CW Nd:YAG laser operating at 1350 W laser mean power. Weld quality was not affected by changes in groove size.« less

Texture Development in a Friction Stir Lap-Welded AZ31B Magnesium Alloy

NASA Astrophysics Data System (ADS)

Naik, B. S.; Chen, D. L.; Cao, X.; Wanjara, P.

2014-09-01

The present study was aimed at characterizing the microstructure, texture, hardness, and tensile properties of an AZ31B-H24 Mg alloy that was friction stir lap welded (FSLWed) at varying tool rotational rates and welding speeds. Friction stir lap welding (FSLW) resulted in the presence of recrystallized grains and an associated hardness drop in the stir zone (SZ). Microstructural investigation showed that both the AZ31B-H24 Mg base metal (BM) and SZ contained β-Mg17Al12 and Al8Mn5 second phase particles. The AZ31B-H24 BM contained a type of basal texture (0001)<110> with the (0001) plane nearly parallel to the rolled sheet surface and <110> directions aligned in the rolling direction. FSLW resulted in the formation of another type of basal texture (0001)<100> in the SZ, where the basal planes (0001) became slightly tilted toward the transverse direction, and the prismatic planes (100) and pyramidal planes (101) exhibited a 30 deg + ( n - 1) × 60 deg rotation ( n = 1, 2, 3, …) with respect to the rolled sheet normal direction, due to the shear plastic flow near the pin surface that occurred from the intense local stirring. With increasing tool rotational rate and decreasing welding speed, the maximum intensity of the basal poles (0001) in the SZ decreased due to a higher degree of dynamic recrystallization that led to a weaker or more random texture. The tool rotational rate and welding speed had a strong effect on the failure load of FSLWed joints. A combination of relatively high welding speed (20 mm/s) and low tool rotational rate (1000 rpm) was observed to be capable of achieving a high failure load. This was attributed to the relatively small recrystallized grains and high intensity of the basal poles in the SZ arising from the low heat input as well as the presence of a small hooking defect.

Lamina-associated polypeptide 2alpha loss impairs heart function and stress response in mice.

PubMed

Gotic, Ivana; Leschnik, Michael; Kolm, Ursula; Markovic, Mato; Haubner, Bernhard J; Biadasiewicz, Katarzyna; Metzler, Bernhard; Stewart, Colin L; Foisner, Roland

2010-02-05

Lamina-associated polypeptide (LAP)2alpha is a mammalian chromatin-binding protein that interacts with a fraction of A-type lamins in the nuclear interior. Because mutations in lamins and LAP2alpha lead to cardiac disorders in humans, we hypothesized that these factors may play important roles in heart development and adult tissue homeostasis. We asked whether the presence of LAP2alpha was required for normal cardiac function. To study the molecular mechanisms of the disease, we analyzed heart structure and function in complete and conditional Lap2alpha(-/-) mice as well as Lap2alpha(-/-)/Mdx mutants. Unlike conditional deletion of LAP2alpha in late embryonic striated muscle, its complete knockout caused systolic dysfunction in young mice, accompanied by sporadic fibrosis in old animals, as well as deregulation of major cardiac transcription factors GATA4 and myocyte enhancer factor 2c. Activation of compensatory pathways, including downregulation of beta-adrenergic receptor signaling, resulted in reduced responsiveness of the myocardium to chronic beta-adrenergic stimulation and stalled the progression of LAP2alpha-deficient hearts from hypertrophy toward cardiac failure. Dystrophin deficiency in an Mdx background resulted in a transient rescue of the Lap2alpha(-/-) phenotype. Our data suggest a novel role of LAP2alpha in the maintenance of cardiac function under normal and stress conditions.

Lipid accumulation product is related to metabolic syndrome in women with polycystic ovary syndrome.

PubMed

Xiang, S; Hua, F; Chen, L; Tang, Y; Jiang, X; Liu, Z

2013-02-01

Metabolic disturbances are common features of polycystic ovary syndrome (PCOS), which possibly enhance the risk of diabetes and cardiovascular disease. Lipid accumulation product (LAP) is an emerging cardiovascular risk factor. The aim of this study was to explore the ability of LAP to identify metabolic syndrome (MS) in PCOS women. In a cross-sectional study, anthropometric, biochemical and clinical parameters were measured in 105 PCOS women. Receiver operating characteristic (ROC) analysis was used to find out the cut-off points of LAP to predict MS. MS was categorized according to International Diabetes Federation (IDF) criteria. The prevalence of MS was 43.8% in this study. PCOS women with MS had significantly higher LAP levels compared to those without MS. LAP was highly correlated with components of MS. ROC analysis showed that LAP was a significant discriminator for MS in PCOS women, and the optimal cutoff point of LAP to predict MS was 54.2 (93.3% sensitivity, 96.7% specificity). LAP seems to be associated with MS and has a strong and reliable diagnostic accuracy for MS in PCOS women. © J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York.

The Staphylococcus aureus leucine aminopeptidase LAP is localized to the bacterial cytosol and demonstrates a broad substrate range that extends beyond leucine

PubMed Central

Carroll, Ronan K.; Veillard, Florian; Gagne, Danielle T.; Lindenmuth, Jarrod M.; Poreba, Marcin; Drag, Marcin; Potempa, Jan; Shaw, Lindsey N.

2013-01-01

Staphylococcus aureus is a potent pathogen of humans exhibiting a broad disease range, in part, due to an extensive repertoire of secreted virulence factors, including proteases. Recently, we identified the first example of an intracellular protease (leucine aminopeptidase - LAP) that is required for virulence in S. aureus. Disruption of pepZ, the gene encoding LAP, had no affect on the growth rate of bacteria, however, in systemic and localized infection models the pepZ mutant was significantly attenuated in virulence. Recently, a contradictory report has been published, suggesting that LAP is an extracellular enzyme and it is required for growth in S. aureus. Here, we investigate these results and confirm our previous findings that LAP is localized to the bacterial cytosol and is not required for growth. In addition we conduct a biochemical investigation of purified recombinant LAP identifying optimal conditions for enzymatic activity and substrate preference for hydrolysis. Our results show that LAP has a broad substrate range, including activity against the dipeptide cysteine-glycine and that leucine is not the primary target of LAP. PMID:23241672

Nonlinear evolution of the Kelvin-Helmholtz instability in the double current sheet configuration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mao, Aohua; Li, Jiquan, E-mail: lijq@energy.kyoto-u.ac.jp; Kishimoto, Yasuaki

2016-03-15

The nonlinear evolution of the Kelvin-Helmholtz (KH) instability driven by a radially antisymmetric shear flow in the double current sheet configuration is numerically investigated based on a reduced magnetohydrodynamic model. Simulations reveal different nonlinear fate of the KH instability depending on the amplitude of the shear flow, which restricts the strength of the KH instability. For strong shear flows far above the KH instability threshold, the linear electrostatic-type KH instability saturates and achieves a vortex flow dominated quasi-steady state of the electromagnetic (EM) KH turbulence with large-amplitude zonal flows as well as zonal fields. The magnetic surfaces are twisted significantlymore » due to strong vortices but without the formation of magnetic islands. However, for the shear flow just over the KH instability threshold, a weak EM-type KH instability is saturated and remarkably damped by zonal flows through modifying the equilibrium shear flow. Interestingly, a secondary double tearing mode (DTM) is excited subsequently in highly damped KH turbulence, behaving as a pure DTM in a flowing plasma as described in Mao et al. [Phys. Plasmas 21, 052304 (2014)]. However, the explosive growth phenomenon is replaced by a gradually growing oscillation due to the extremely twisted islands. As a result, the release of the magnetic energy becomes slow and the global magnetic reconnection tends to be gentle. A complex nonlinear interaction between the EM KH turbulence and the DTMs occurs for the medium shear flows above the KH instability threshold, turbulent EM fluctuations experience oscillatory nonlinear growth of the DTMs, finally achieves a quasi-steady state with the interplay of the fluctuations between the DTMs and the EM KH instability.« less

Experimental characterization and numerical simulation of riveted lap-shear joints using Rivet Element

NASA Astrophysics Data System (ADS)

Vivio, Francesco; Fanelli, Pierluigi; Ferracci, Michele

2018-03-01

In aeronautical and automotive industries the use of rivets for applications requiring several joining points is now very common. In spite of a very simple shape, a riveted junction has many contact surfaces and stress concentrations that make the local stiffness very difficult to be calculated. To overcome this difficulty, commonly finite element models with very dense meshes are performed for single joint analysis because the accuracy is crucial for a correct structural analysis. Anyhow, when several riveted joints are present, the simulation becomes computationally too heavy and usually significant restrictions to joint modelling are introduced, sacrificing the accuracy of local stiffness evaluation. In this paper, we tested the accuracy of a rivet finite element presented in previous works by the authors. The structural behaviour of a lap joint specimen with a rivet joining is simulated numerically and compared to experimental measurements. The Rivet Element, based on a closed-form solution of a reference theoretical model of the rivet joint, simulates local and overall stiffness of the junction combining high accuracy with low degrees of freedom contribution. In this paper the Rivet Element performances are compared to that of a FE non-linear model of the rivet, built with solid elements and dense mesh, and to experimental data. The promising results reported allow to consider the Rivet Element able to simulate, with a great accuracy, actual structures with several rivet connections.

Effects of shear on the magnetic footprint and stochastic layer in double-null divertor tokamak

NASA Astrophysics Data System (ADS)

Farhat, Hamidullah; Punjabi, Alkesh; Ali, Halima

2006-10-01

We have developed a new area-preserving map, called the Adjustable Shear Map, to calculate effects of shear on the magnetic footprint and stochastic layer in double-null divertor tokamak. The map is given by equationsxn+1=xn-kyn[(1-yn^2 )(1+syn)+sxn+1^2 ),yn+1=yn+kxn+1[1+s(xn+1^2 +yn^2 )]. k is the map parameter and s is the shear parameter. O-point of the map is (0, 0), and the X-points are (0, 1), and (0, -1). For s=0, k=0.6, the last good surface is y=0.9918 with q ˜3. Here we will report on the effects of shear on the stochastic layer and magnetic footprint as the shear parameter is varied from 0 to -1. Here we will report the preliminary results on the effect of shear on the magnetic foot print and the stochastic layer where the shear parameter s has values between -1 and 0. using method of maps [1-4]. This work is done under the DOE grant number DE-FG02-01ER54624. 1. A. Punjabi, A. Boozer, and A. Verma, Phys. Rev. lett., 69, 3322 (1992). 2. H. Ali, A. Punjabi, and A. Boozer, Phys. Plasmas 11, 4527 (2004). 3. A. Punjabi, H. Ali, and A. Boozer, Phys. Plasmas 10, 3992 (2003). 4. A. Punjabi, H. Ali, and A. Boozer, Phys. Plasmas 4, 337 (1997).

Quantification of wall shear stress in large blood vessels using Lagrangian interpolation functions with cine phase-contrast magnetic resonance imaging.

PubMed

Cheng, Christopher P; Parker, David; Taylor, Charles A

2002-09-01

Arterial wall shear stress is hypothesized to be an important factor in the localization of atherosclerosis. Current methods to compute wall shear stress from magnetic resonance imaging (MRI) data do not account for flow profiles characteristic of pulsatile flow in noncircular vessel lumens. We describe a method to quantify wall shear stress in large blood vessels by differentiating velocity interpolation functions defined using cine phase-contrast MRI data on a band of elements in the neighborhood of the vessel wall. Validation was performed with software phantoms and an in vitro flow phantom. At an image resolution corresponding to in vivo imaging data of the human abdominal aorta, time-averaged, spatially averaged wall shear stress for steady and pulsatile flow were determined to be within 16% and 23% of the analytic solution, respectively. These errors were reduced to 5% and 8% with doubling in image resolution. For the pulsatile software phantom, the oscillation in shear stress was predicted to within 5%. The mean absolute error of circumferentially resolved shear stress for the nonaxisymmetric phantom decreased from 28% to 15% with a doubling in image resolution. The irregularly shaped phantom and in vitro investigation demonstrated convergence of the calculated values with increased image resolution. We quantified the shear stress at the supraceliac and infrarenal regions of a human abdominal aorta to be 3.4 and 2.3 dyn/cm2, respectively.

Cursory examination of the zeta potential behaviors of two optical materials

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tesar, A.; Oja, T.

1992-01-02

When an oxide surface is placed in water, a difference in potential across the interface occurs due to dipole orientation. Hydroxyl groups or bound oxygen atoms on the oxide surface will orient adjacent water molecules which balance the dipole charge. This occurs over some small distance called the electrical double layer. Trace amounts of high field strength ions present in the vicinity of the double layer can have significant effects on the double layer. When there is movement of the oxide surface with respect to the water, a shearing of the double layer occurs. The electrical potential at this surfacemore » of shear is termed the zeta potential. The impetus for this study was to document the zeta potential behavior in water of two optical materials. (1) a multicomponent phosphate glass; and (2) Zerodur, a silicate glass-ceramic.« less

Effect of shear stress on cell cultures and other reactor problems

NASA Technical Reports Server (NTRS)

Schleier, H.

1981-01-01

Anchorage dependent cell cultures in fluidized beds are tested. Feasibility calculations indicate the allowed parameters and estimate the shear stresses therein. In addition, the diffusion equation with first order reaction is solved for the spherical shell (double bubble) reactor with various constraints.

A Study of the Effects of Electrode Number and Decoding Algorithm on Online EEG-Based BCI Behavioral Performance

PubMed Central

Meng, Jianjun; Edelman, Bradley J.; Olsoe, Jaron; Jacobs, Gabriel; Zhang, Shuying; Beyko, Angeliki; He, Bin

2018-01-01

Motor imagery–based brain–computer interface (BCI) using electroencephalography (EEG) has demonstrated promising applications by directly decoding users' movement related mental intention. The selection of control signals, e.g., the channel configuration and decoding algorithm, plays a vital role in the online performance and progressing of BCI control. While several offline analyses report the effect of these factors on BCI accuracy for a single session—performance increases asymptotically by increasing the number of channels, saturates, and then decreases—no online study, to the best of our knowledge, has yet been performed to compare for a single session or across training. The purpose of the current study is to assess, in a group of forty-five subjects, the effect of channel number and decoding method on the progression of BCI performance across multiple training sessions and the corresponding neurophysiological changes. The 45 subjects were divided into three groups using Laplacian Filtering (LAP/S) with nine channels, Common Spatial Pattern (CSP/L) with 40 channels and CSP (CSP/S) with nine channels for online decoding. At the first training session, subjects using CSP/L displayed no significant difference compared to CSP/S but a higher average BCI performance over those using LAP/S. Despite the average performance when using the LAP/S method was initially lower, but LAP/S displayed improvement over first three sessions, whereas the other two groups did not. Additionally, analysis of the recorded EEG during BCI control indicates that the LAP/S produces control signals that are more strongly correlated with the target location and a higher R-square value was shown at the fifth session. In the present study, we found that subjects' average online BCI performance using a large EEG montage does not show significantly better performance after the first session than a smaller montage comprised of a common subset of these electrodes. The LAP/S method with a small EEG montage allowed the subjects to improve their skills across sessions, but no improvement was shown for the CSP method. PMID:29681792

A Study of the Effects of Electrode Number and Decoding Algorithm on Online EEG-Based BCI Behavioral Performance.

PubMed

Meng, Jianjun; Edelman, Bradley J; Olsoe, Jaron; Jacobs, Gabriel; Zhang, Shuying; Beyko, Angeliki; He, Bin

2018-01-01

Motor imagery-based brain-computer interface (BCI) using electroencephalography (EEG) has demonstrated promising applications by directly decoding users' movement related mental intention. The selection of control signals, e.g., the channel configuration and decoding algorithm, plays a vital role in the online performance and progressing of BCI control. While several offline analyses report the effect of these factors on BCI accuracy for a single session-performance increases asymptotically by increasing the number of channels, saturates, and then decreases-no online study, to the best of our knowledge, has yet been performed to compare for a single session or across training. The purpose of the current study is to assess, in a group of forty-five subjects, the effect of channel number and decoding method on the progression of BCI performance across multiple training sessions and the corresponding neurophysiological changes. The 45 subjects were divided into three groups using Laplacian Filtering (LAP/S) with nine channels, Common Spatial Pattern (CSP/L) with 40 channels and CSP (CSP/S) with nine channels for online decoding. At the first training session, subjects using CSP/L displayed no significant difference compared to CSP/S but a higher average BCI performance over those using LAP/S. Despite the average performance when using the LAP/S method was initially lower, but LAP/S displayed improvement over first three sessions, whereas the other two groups did not. Additionally, analysis of the recorded EEG during BCI control indicates that the LAP/S produces control signals that are more strongly correlated with the target location and a higher R-square value was shown at the fifth session. In the present study, we found that subjects' average online BCI performance using a large EEG montage does not show significantly better performance after the first session than a smaller montage comprised of a common subset of these electrodes. The LAP/S method with a small EEG montage allowed the subjects to improve their skills across sessions, but no improvement was shown for the CSP method.

A cost comparison of laparoscopic and open colon surgery in a publicly funded academic institution.

PubMed

Hardy, Krista M; Kwong, Josephine; Pitzul, Kristen B; Vergis, Ashley S; Jackson, Timothy D; Urbach, David R; Okrainec, Allan

2014-04-01

The objective of this study was to compare the total hospital cost of laparoscopic (lap) and open colon surgery at a publicly funded academic institution. Patients undergoing elective laparoscopic or open colon surgery for all indications at the University Health Network, Toronto, Canada, from April 2004 to March 2009 were included. Patient demographic, operative, and outcome data were reviewed retrospectively. Hospital costs were determined from the Ontario Case Costing Initiative, adjusted for inflation, and compared using the Mann-Whitney U test. Linear regression was used to analyze the relationship between length of stay and total hospital cost. There were 391 elective colon resections (223 lap/168 open, 15.4 % conversion). There was no difference in median age, gender, or Charlson score. Body mass index was slightly higher for laparoscopic surgery (27.5/25.9 lap/open; p = 0.008), while the American Society of Anesthesiologists score was slightly higher for open surgery. Median operative time was greater for laparoscopic surgery (224/196 min, lap/open; p = 0.001). There was no difference in complication rates (21.6/22.5 % lap/open; p = 0.900), reoperations (5.8/6.5 % lap/open; p = 0.833) or 30-day readmissions (7.6/12.5 % lap/open; p = 0.122). Number of emergency room visits was greater with open surgery (12.6/20.8 % lap/open; p = 0.037). Operative cost was higher for laparoscopic surgery ($4,171.37/3,489.29 lap/open; p = 0.001), while total hospital cost was significantly reduced ($9,600.22/12,721.41 lap/open; p = 0.001). Median length of stay was shorter for laparoscopic surgery (5/7 days lap/open; p = 0.000), and this correlated directly with hospital cost. Laparoscopic colon surgery is associated with increased operative costs but significantly lower total hospital costs. The cost savings is related, in part, to reduced length of stay with laparoscopic surgery.

23 CFR 1215.3 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-04-01

... consisting of a lap belt or a lap belt and a detachable shoulder belt; and with respect to other passenger vehicles, an occupant restraint system consisting of integrated lap shoulder belts. Secretary means the...

23 CFR 1215.3 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-04-01

... consisting of a lap belt or a lap belt and a detachable shoulder belt; and with respect to other passenger vehicles, an occupant restraint system consisting of integrated lap shoulder belts. Secretary means the...

23 CFR 1215.3 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-04-01

... consisting of a lap belt or a lap belt and a detachable shoulder belt; and with respect to other passenger vehicles, an occupant restraint system consisting of integrated lap shoulder belts. Secretary means the...

23 CFR 1215.3 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... consisting of a lap belt or a lap belt and a detachable shoulder belt; and with respect to other passenger vehicles, an occupant restraint system consisting of integrated lap shoulder belts. Secretary means the...

23 CFR 1215.3 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-04-01

... consisting of a lap belt or a lap belt and a detachable shoulder belt; and with respect to other passenger vehicles, an occupant restraint system consisting of integrated lap shoulder belts. Secretary means the...

A strong association between lipid accumulation product and diabetes mellitus in japanese women and men.

PubMed

Wakabayashi, Ichiro; Daimon, Takashi

2014-01-01

Lipid accumulation product (LAP) is a new continuous marker of lipid overaccumulation that predicts cardiovascular risk. The aim of this study was to determine the cutoff value for LAP and evaluate its usefulness. Using a database of results of health checkup examinations for 10,170 Japanese workers (35-40 years of age) conducted at their workplaces, the cutoff value for a high LAP was calculated by analyzing receiver-operating characteristic (ROC) curves for the relationships of LAP with hyperglycemia and diabetes. The cutoff value for LAP was 21.1 for women and 37.2 for men. The values were similar when calculated by analyzing the ROC curves for the relationships with hyperglycemia and diabetes. Using these cutoff values, the prevalence of a high LAP was calculated to be 23.7% in women and 28.8% in men. The odds ratio for diabetes in the subjects with vs. those without a high LAP, calculated after adjusting for age, smoking, alcohol consumption and regular exercise, was 19.09 (95% CI: 6.57-55.50) in women and 7.40 (95% CI: 5.10-10.75) in men. High odds ratios for hypertension (10.66 [95% CI: 7.77-14.63] in women and 7.31 [95% CI: 6.20-8.62] in men) were also obtained in the subjects with vs. those without a high LAP. Cutoff values for a high LAP in women and men were determined, and high odds ratios for diabetes and hypertension were obtained using the cutoff values for LAP. Further studies are needed to elucidate whether the proposed cutoff values are applicable to people of other ages, races and ethnicities.

15 CFR 285.4 - Establishment of laboratory accreditation programs (LAPs) within NVLAP.

Code of Federal Regulations, 2010 CFR

2010-01-01

... legislative actions or to requests from private sector entities and government agencies. For legislatively mandated LAPs, NVLAP shall establish the LAP. For requests from private sector entities and government...

Hsp72 protects against liver injury via attenuation of hepatocellular death, oxidative stress, and JNK signaling.

PubMed

Levada, Kateryna; Guldiken, Nurdan; Zhang, Xiaoji; Vella, Giovanna; Mo, Fa-Rong; James, Laura P; Haybaeck, Johannes; Kessler, Sonja M; Kiemer, Alexandra K; Ott, Thomas; Hartmann, Daniel; Hüser, Norbert; Ziol, Marianne; Trautwein, Christian; Strnad, Pavel

2018-05-01

Heat shock protein (Hsp) 72 is a molecular chaperone that has broad cytoprotective functions and is upregulated in response to stress. To determine its hepatic functions, we studied its expression in human liver disorders and its biological significance in newly generated transgenic animals. Double transgenic mice overexpressing Hsp72 (gene Hspa1a) under the control of a tissue-specific tetracycline-inducible system (Hsp72-LAP mice) were produced. Acute liver injury was induced by a single injection of acetaminophen (APAP). Feeding with either a methionine choline-deficient (MCD; 8 weeks) or a 3,5-diethoxycarbonyl-1,4-dihydrocollidine-supplemented diet (DDC; 12 weeks) was used to induce lipotoxic injury and Mallory-Denk body (MDB) formation, respectively. Primary hepatocytes were treated with palmitic acid. Patients with non-alcoholic steatohepatitis and chronic hepatitis C infection displayed elevated HSP72 levels. These levels increased with the extent of hepatic inflammation and HSP72 expression was induced after treatment with either interleukin (IL)-1β or IL-6. Hsp72-LAP mice exhibited robust, hepatocyte-specific Hsp72 overexpression. Primary hepatocytes from these animals were more resistant to isolation-induced stress and Hsp72-LAP mice displayed lower levels of hepatic injury in vivo. Mice overexpressing Hsp72 had fewer APAP protein adducts and were protected from oxidative stress and APAP-/MCD-induced cell death. Hsp72-LAP mice and/or hepatocytes displayed significantly attenuated Jnk activation. Overexpression of Hsp72 did not affect steatosis or the extent of MDB formation. Our results demonstrate that HSP72 induction occurs in human liver disease, thus, HSP72 represents an attractive therapeutic target owing to its broad hepatoprotective functions. HSP72 constitutes a stress-inducible, protective protein. Our data demonstrate that it is upregulated in patients with chronic hepatitis C and non-alcoholic steatohepatitis. Moreover, Hsp72-overexpressing mice are protected from various forms of liver stress. Copyright © 2018 European Association for the Study of the Liver. All rights reserved.

Design fabrication and test of graphite/polyimide composite joints and attachments for advanced aerospace vehicles

NASA Technical Reports Server (NTRS)

1979-01-01

Graphite/polyimide (Gr/PI) bolted and bonded joints were investigated. Possible failure modes and the design loads for the four generic joint types are discussed. Preliminary sizing of a type 1 joint, bonded and bolted configuration is described, including assumptions regarding material properties and sizing methodology. A general purpose finite element computer code is described that was formulated to analyze single and double lap joints, with and without tapered adherends, and with user-controlled variable element size arrangements. An initial order of Celion 6000/PMR-15 prepreg was received and characterized.

Nonlinear stability research on the hydraulic system of double-side rolling shear.

PubMed

Wang, Jun; Huang, Qingxue; An, Gaocheng; Qi, Qisong; Sun, Binyu

2015-10-01

This paper researches the stability of the nonlinear system taking the hydraulic system of double-side rolling shear as an example. The hydraulic system of double-side rolling shear uses unsymmetrical electro-hydraulic proportional servo valve to control the cylinder with single piston rod, which can make best use of the space and reduce reversing shock. It is a typical nonlinear structure. The nonlinear state-space equations of the unsymmetrical valve controlling cylinder system are built first, and the second Lyapunov method is used to evaluate its stability. Second, the software AMEsim is applied to simulate the nonlinear system, and the results indicate that the system is stable. At last, the experimental results show that the system unsymmetrical valve controlling the cylinder with single piston rod is stable and conforms to what is deduced by theoretical analysis and simulation. The construction and application of Lyapunov function not only provide the theoretical basis for using of unsymmetrical valve controlling cylinder with single piston rod but also develop a new thought for nonlinear stability evaluation.

Nonlinear stability research on the hydraulic system of double-side rolling shear

NASA Astrophysics Data System (ADS)

Wang, Jun; Huang, Qingxue; An, Gaocheng; Qi, Qisong; Sun, Binyu

2015-10-01

This paper researches the stability of the nonlinear system taking the hydraulic system of double-side rolling shear as an example. The hydraulic system of double-side rolling shear uses unsymmetrical electro-hydraulic proportional servo valve to control the cylinder with single piston rod, which can make best use of the space and reduce reversing shock. It is a typical nonlinear structure. The nonlinear state-space equations of the unsymmetrical valve controlling cylinder system are built first, and the second Lyapunov method is used to evaluate its stability. Second, the software AMEsim is applied to simulate the nonlinear system, and the results indicate that the system is stable. At last, the experimental results show that the system unsymmetrical valve controlling the cylinder with single piston rod is stable and conforms to what is deduced by theoretical analysis and simulation. The construction and application of Lyapunov function not only provide the theoretical basis for using of unsymmetrical valve controlling cylinder with single piston rod but also develop a new thought for nonlinear stability evaluation.

True Shear Parallel Plate Viscometer

NASA Technical Reports Server (NTRS)

Ethridge, Edwin; Kaukler, William

2010-01-01

This viscometer (which can also be used as a rheometer) is designed for use with liquids over a large temperature range. The device consists of horizontally disposed, similarly sized, parallel plates with a precisely known gap. The lower plate is driven laterally with a motor to apply shear to the liquid in the gap. The upper plate is freely suspended from a double-arm pendulum with a sufficiently long radius to reduce height variations during the swing to negligible levels. A sensitive load cell measures the shear force applied by the liquid to the upper plate. Viscosity is measured by taking the ratio of shear stress to shear rate.

Melanin targets LC3-associated phagocytosis (LAP): A novel pathogenetic mechanism in fungal disease.

PubMed

Chamilos, Georgios; Akoumianaki, Tonia; Kyrmizi, Irene; Brakhage, Axel; Beauvais, Anne; Latge, Jean-Paul

2016-05-03

Intracellular swelling of conidia of the major human airborne fungal pathogen Aspergillus fumigatus results in surface exposure of immunostimulatory pathogen-associated molecular patterns (PAMPs) and triggers activation of a specialized autophagy pathway called LC3-associated phagocytosis (LAP) to promote fungal killing. We have recently discovered that, apart from PAMPs exposure, cell wall melanin removal during germination of A. fumigatus is a prerequisite for activation of LAP. Importantly, melanin promotes fungal pathogenicity via targeting LAP, as a melanin-deficient A. fumigatus mutant restores its virulence upon conditional inactivation of Atg5 in hematopoietic cells of mice. Mechanistically, fungal cell wall melanin selectively excludes the CYBA/p22phox subunit of NADPH oxidase from the phagosome to inhibit LAP, without interfering with signaling regulating cytokine responses. Notably, inhibition of LAP is a general property of melanin pigments, a finding with broad physiological implications.

In-plane and Interlaminar Shear Strength of a Unidirectional Hi-nicalon Fiber-reinforced Celsian Matrix Composite

NASA Technical Reports Server (NTRS)

Uenal, O.; Bansal, N. P.

2000-01-01

In-plane and interlaminar shear strength of a unidirectional SiC fiber-reinforced (BaSr)Al2Si2O8 celsian composite were measured by the double-notch shear test method between room temperature and 1200 C. The interlaminar shear strength was lower than the in-plane shear strength at all temperatures. Stress analysis, using finite element modeling, indicated that shear stress concentration was not responsible for the observed difference in strength. Instead, the difference in layer architecture and thus, the favorable alignment of fiber-rich layers with the shear plane in the interlaminar specimens appears to be the reason for the low strength of this composite. A rapid decrease in strength was observed with temperature due to softening of the glassy phase in the material.

Lateral Earth Pressure at Rest and Shear Modulus Measurements on Hanford Sludge Simulants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wells, Beric E.; Jenks, Jeromy WJ; Boeringa, Gregory K.

2010-09-30

This report describes the equipment, techniques, and results of lateral earth pressure at rest and shear modulus measurements on kaolin clay as well as two chemical sludge simulants. The testing was performed in support of the problem of hydrogen gas retention and release encountered in the double- shell tanks (DSTs) at the Hanford Site near Richland, Washington. Wastes from single-shell tanks (SSTs) are being transferred to double-shell tanks (DSTs) for safety reasons (some SSTs are leaking or are in danger of leaking), but the available DST space is limited.

A new synthetic route to a family of non-classical addition-type thermoplastics. I - Concept and demonstration

NASA Technical Reports Server (NTRS)

Pater, Ruth H.

1988-01-01

A synthesis scheme was developed for a host of nonclassical addition-type thermoplastics (ATT) that can be cured by an addition reaction, leading to a linear polymer structure. The synthesis involves the reaction of an acetylene-terminated prepolymer with either a bismaleimide (BMI) or a biscitraconimide. A new polymer, designated LaRC-RP80, synthesized using this scheme, was found to exhibit several significantly improved properties over the commercial BMI, Kerimid 601, including an eight-fold increase in toughness while maintaining a high Tg, a higher (by 167 C) heat stability, and a 50-percent increase in moisture resistance. In addition, LaRC-RP80 has good hot/wet lap shear strength and processes easily at 288 C without voids in the finished product.

Space fabrication demonstration system. [beam builder and induction fastening

NASA Technical Reports Server (NTRS)

1980-01-01

The development effort on the composite beam cap fabricator was completed within cost and close to abbreviated goals. The design and analysis of flight weight primary and secondary beam builder structures proceeded satisfactorily but remains curtailed until further funding is made available to complete the work. The induction fastening effort remains within cost and schedule constraints. Tests of the LARC prototype induction welder is continuing in an instrumented test stand comprised of a Dumore drill press (air over oil feed for variable applied loads) and a dynamometer to measure actual welding loads. Continued testing shows that the interface screening must be well impregnated with resin to ensure proper flow when bonding graphite-acrylic lap shear samples. Specimens prepared from 0.030 inch thick graphite-polyethersulfone are also available for future induction fastening evaluation.

Adhesive evaluation of LARC-TPI and a water-soluble version of LARC-TPI

NASA Technical Reports Server (NTRS)

Progar, D. J.

1985-01-01

The results of a study to evaluate two Langley Research Center thermoplastic polimide (TPI) materials, identified as TPI/MTC for the material from Mitsui Toatsu Chemicals Inc. and TPI/H2O for the material from United Technologies Research Center, as high temperature thermoplastic adhesives and primers for bonding titanium (6AL-4V) adherends are discussed. A limited characterization of the materials was performed using a Diffuse Reflectance-Fourier Transform Infrared Spectroscopy (DR-FTIR) technique. Thermomechanical Analysis (TMA) and torsional braid techniques were used to determine glass transition temperature. The adhesive's strength, as determined by simple lap shear tests, as used to evaluate the effects of long term thermal exposure (up to 1000 hrs) at 204 deg C and a 72-hour water-boil.

Verification of surface preparation for adhesive bonding

NASA Technical Reports Server (NTRS)

Myers, Rodney S.

1995-01-01

A survey of solid rocket booster (SRB) production operations identified potential contaminants which might adversely affect bonding operations. Lap shear tests quantified these contaminants' effects on adhesive strength. The most potent contaminants were selected for additional studies on SRB thermal protection system (TPS) bonding processes. Test panels were prepared with predetermined levels of contamination, visually inspected using white and black light, then bonded with three different TPS materials over the unremoved contamination. Bond test data showed that white and black light inspections are adequate inspection methods for TPS bonding operations. Extreme levels of contamination (higher than expected on flight hardware) had an insignificant effect on TPS bond strengths because of the apparent insensitivity of the adhesive system to contamination effects, and the comparatively weak cohesive strength of the TPS materials.

Echocardiographic estimation of left atrial pressure in beagle dogs with experimentally-induced mitral valve regurgitation.

PubMed

Ishikawa, Taisuke; Fukushima, Ryuji; Suzuki, Shuji; Miyaishi, Yuka; Nishimura, Taiki; Hira, Satoshi; Hamabe, Lina; Tanaka, Ryou

2011-08-01

Non-invasive and immediate estimation of left atrial pressure (LAP) is very useful for the management of mitral regurgitation (MR), and many reports have assessed echocardiographic estimations of LAP to date. However, it has been unclear of which examination and evaluate article possess the best accuracy for the MR severity. The present research aims to establish the echocardiographic estimation equation of LAP that is well applicable for clinical MR dogs. After the chordae tendineae rupture was experimentally induced via left atriotomy in six healthy beagle dogs (three males and three females, two years old, weighing between 9.8 to 12.8 kg), a radio telemetry transmitter catheter was inserted, which allows the continuous recordings of LAP without the use of sedation. Approximately 5 weeks after the surgery, echocardiographic examination, indirect blood pressure measurement, measurement of plasma atrial natriuretic peptide (ANP) and LAP measurement by way of the radio telemetry system was performed simultaneously. Subsequently, simple linear regression equations between LAP and each variable were obtained, and the equations were evaluated whether to be applicable for clinical MR dogs. As a result, the ratio of early diastolic mitral flow to early diastolic lateral mitral annulus velocity (E/Ea) had the strongest correlation as maximum LAP=7.03*(E/Ea)-54.86 (r=0.74), and as mean LAP=4.94*(E/Ea)-40.37 (r=0.70) among the all variables. Therefore, these two equations associated with E/Ea should bring more precise and instant estimations of maximum and mean LAP in clinical MR dogs.

Laboratory observations of fault strength in response to changes in normal stress

USGS Publications Warehouse

Kilgore, Brian D.; Lozos, Julian; Beeler, Nicholas M.; Oglesby, David

2012-01-01

Changes in fault normal stress can either inhibit or promote rupture propagation, depending on the fault geometry and on how fault shear strength varies in response to the normal stress change. A better understanding of this dependence will lead to improved earthquake simulation techniques, and ultimately, improved earthquake hazard mitigation efforts. We present the results of new laboratory experiments investigating the effects of step changes in fault normal stress on the fault shear strength during sliding, using bare Westerly granite samples, with roughened sliding surfaces, in a double direct shear apparatus. Previous experimental studies examining the shear strength following a step change in the normal stress produce contradictory results: a set of double direct shear experiments indicates that the shear strength of a fault responds immediately, and then is followed by a prolonged slip-dependent response, while a set of shock loading experiments indicates that there is no immediate component, and the response is purely gradual and slip-dependent. In our new, high-resolution experiments, we observe that the acoustic transmissivity and dilatancy of simulated faults in our tests respond immediately to changes in the normal stress, consistent with the interpretations of previous investigations, and verify an immediate increase in the area of contact between the roughened sliding surfaces as normal stress increases. However, the shear strength of the fault does not immediately increase, indicating that the new area of contact between the rough fault surfaces does not appear preloaded with any shear resistance or strength. Additional slip is required for the fault to achieve a new shear strength appropriate for its new loading conditions, consistent with previous observations made during shock loading.

Transfer of skills on LapSim virtual reality laparoscopic simulator into the operating room in urology.

PubMed

Alwaal, Amjad; Al-Qaoud, Talal M; Haddad, Richard L; Alzahrani, Tarek M; Delisle, Josee; Anidjar, Maurice

2015-01-01

Assessing the predictive validity of the LapSim simulator within a urology residency program. Twelve urology residents at McGill University were enrolled in the study between June 2008 and December 2011. The residents had weekly training on the LapSim that consisted of 3 tasks (cutting, clip-applying, and lifting and grasping). They underwent monthly assessment of their LapSim performance using total time, tissue damage and path length among other parameters as surrogates for their economy of movement and respect for tissue. The last residents' LapSim performance was compared with their first performance of radical nephrectomy on anesthetized porcine models in their 4(th) year of training. Two independent urologic surgeons rated the resident performance on the porcine models, and kappa test with standardized weight function was used to assess for inter-observer bias. Nonparametric spearman correlation test was used to compare each rater's cumulative score with the cumulative score obtained on the porcine models in order to test the predictive validity of the LapSim simulator. The kappa results demonstrated acceptable agreement between the two observers among all domains of the rating scale of performance except for confidence of movement and efficiency. In addition, poor predictive validity of the LapSim simulator was demonstrated. Predictive validity was not demonstrated for the LapSim simulator in the context of a urology residency training program.

EEG and ERP profiles in the high alcohol preferring (HAP) and low alcohol preferring (LAP) mice: relationship to ethanol preference.

PubMed

Slawecki, Craig J; Grahame, Nicholas J; Roth, Jennifer; Katner, Simon N; Ehlers, C L

2003-01-31

Neurophysiological measures, such as decreased P300 amplitude and altered EEG alpha activity, have been associated with increased alcoholism risk. The purpose of the present study was to extend the assessment of the neurophysiological indices associated with alcohol consumption to a recently developed mouse model of high ethanol consumption, the first replicate line of high alcohol preferring (HAP-1) and low alcohol preferring (LAP-1) mice. Male HAP-1, LAP-1, and HS mice from the Institute for Behavioral Genetics at the University of Colorado Health Science Center (i.e., HS/Ibg mice) were implanted with cortical electrodes. EEG activity, and event related potentials (ERPs) were then examined. Following electrophysiological assessment, ethanol preference was assessed to examine the relationship between neurophysiological indices and ethanol consumption. EEG analyses revealed that HAPs and HS/Ibgs had greater peak frequency in the 2-4-Hz band and lower peak frequency in the 6-8- and 1-50-Hz bands of the cortical EEG compared to LAPs. Compared to HAPs, LAPs and HS/Ibgs had decreased peak EEG frequency in the 8-16-Hz band. Decreased parietal cortical power from 8 to 50 Hz was associated with high initial ethanol preference in HAP mice. In regards to ERPs, P1 amplitude was greater in HAPs compared to both LAPs and HS/Ibgs and the P3 latency in LAPs was decreased compared to both HAPs and HS/Ibgs. As expected, HAPs consumed more ethanol and had higher ethanol preference than LAPs and HS/Ibgs. There were no significant differences in ethanol intake or preference between HS/Ibgs and LAPs. These data indicate that selective breeding of the HAP and LAP lines has resulted in the divergence of EEG and ERP phenotypes. The differences observed suggest that increased cortical P1 amplitude and altered cortical EEG activity in the 8-50-Hz frequency range may be neurophysiological 'risk factors' associated with high ethanol consumption in mice. Decreased P3 latency in LAPs compared to HAPs and HS/Ibgs mice may be a 'protective factor'.

Laparoscopic Skills Are Improved With LapMentor™ Training

PubMed Central

Andreatta, Pamela B.; Woodrum, Derek T.; Birkmeyer, John D.; Yellamanchilli, Rajani K.; Doherty, Gerard M.; Gauger, Paul G.; Minter, Rebecca M.

2006-01-01

Objective: To determine if prior training on the LapMentor™ laparoscopic simulator leads to improved performance of basic laparoscopic skills in the animate operating room environment. Summary Background Data: Numerous influences have led to the development of computer-aided laparoscopic simulators: a need for greater efficiency in training, the unique and complex nature of laparoscopic surgery, and the increasing demand that surgeons demonstrate competence before proceeding to the operating room. The LapMentor™ simulator is expensive, however, and its use must be validated and justified prior to implementation into surgical training programs. Methods: Nineteen surgical interns were randomized to training on the LapMentor™ laparoscopic simulator (n = 10) or to a control group (no simulator training, n = 9). Subjects randomized to the LapMentor™ trained to expert criterion levels 2 consecutive times on 6 designated basic skills modules. All subjects then completed a series of laparoscopic exercises in a live porcine model, and performance was assessed independently by 2 blinded reviewers. Time, accuracy rates, and global assessments of performance were recorded with an interrater reliability between reviewers of 0.99. Results: LapMentor™ trained interns completed the 30° camera navigation exercise in significantly less time than control interns (166 ± 52 vs. 220 ± 39 seconds, P < 0.05); they also achieved higher accuracy rates in identifying the required objects with the laparoscope (96% ± 8% vs. 82% ± 15%, P < 0.05). Similarly, on the two-handed object transfer exercise, task completion time for LapMentor™ trained versus control interns was 130 ± 23 versus 184 ± 43 seconds (P < 0.01) with an accuracy rate of 98% ± 5% versus 80% ± 13% (P < 0.001). Additionally, LapMentor™ trained interns outperformed control subjects with regard to camera navigation skills, efficiency of motion, optimal instrument handling, perceptual ability, and performance of safe electrocautery. Conclusions: This study demonstrates that prior training on the LapMentor™ laparoscopic simulator leads to improved resident performance of basic skills in the animate operating room environment. This work marks the first prospective, randomized evaluation of the LapMentor™ simulator, and provides evidence that LapMentor™ training may lead to improved operating room performance. PMID:16772789

Adhesion strength of norbornene-based self-healing agents to an amine-cured epoxy

NASA Astrophysics Data System (ADS)

Huang, Guang Chun; Lee, Jong Keun; Kessler, Michael R.; Yoon, Sungho

2009-07-01

Self-healing is triggered by crack propagation through embedded microcapsules in an epoxy matrix, which then release the liquid healing agent into the crack plane. Subsequent exposure of the healing agent to the chemical catalyst initiates ring-opening metathesis polymerization (ROMP) and bonding of the crack faces. In order to improve self-healing functionality, it is necessary to enhance adhesion of polymerized healing agent within the crack to the matrix resin. In this study, shear bond strength between different norbornene-based healing agents and an amine-cured epoxy resin was evaluated using the single lap shear test method (ASTM D3163, modified). The healing agents tested include endodicyclopentadiene (endo-DCPD), 5-ethylidene-2-norbornene (ENB) and DCPD/ENB blends. 5-Norbornene-2-methanol (NBM) was used as an adhesion promoter, containing hydroxyl groups to form hydrogen bonds with the amine-cured epoxy. A custom synthesized norbornene-based crosslinking agent was also added to improve adhesion for ENB by increasing the crosslinking density of the adhesive after ROMP. The healing agents were polymerized with varying loadings of the 1st generation Grubbs' catalyst at different reaction times and temperatures.

An Experimental Study on Micro Clinching of Metal Foils with Cutting by Laser Shock Forming.

PubMed

Wang, Xiao; Li, Cong; Ma, Youjuan; Shen, Zongbao; Sun, Xianqing; Sha, Chaofei; Gao, Shuai; Li, Liyin; Liu, Huixia

2016-07-13

This paper describes a novel technique for joining similar and dissimilar metal foils, namely micro clinching with cutting by laser shock forming. A series of experiments were conducted to study the deformation behavior of single layer material, during which many important process parameters were determined. The process window of the 1060 pure aluminum foils and annealed copper foils produced by micro clinching with cutting was analyzed. Moreover, similar material combination (annealed copper foils) and dissimilar material combination (1060 pure aluminum foils and 304 stainless steel foils) were successfully achieved. The effect of laser energy on the interlock and minimum thickness of upper foils was investigated. In addition, the mechanical strength of different material combinations joined by micro clinching with cutting was measured in single lap shearing tests. According to the achieved results, this novel technique is more suitable for material combinations where the upper foil is thicker than lower foil. With the increase of laser energy, the interlock increased while the minimum thickness of upper foil decreased gradually. The shear strength of 1060 pure aluminum foils and 304 stainless steel foils combination was three times as large as that of 1060 pure aluminum foils and annealed copper foils combination.

Development of damage suppression system using embedded SMA foil sensor and actuator

NASA Astrophysics Data System (ADS)

Ogisu, Toshimichi; Nomura, Masato; Ando, Norio; Takaki, Junji; Song, Dong Y.; Takeda, Nobuo

2000-06-01

The recent studies suggest possible applications of shape memory alloy (SMA) for a smart health monitoring and suppression of damage growth. The authors have been conducting research and development studies on applications of embedded SMA foil sensors and actuators in CFRP laminates. The goal of this research is suppression of damage growth in CFRP laminates. At first, the authors proposed a concept of damage suppression in CFRP laminates. Then, the development studies are conducted in three phases. The first phase is the improvement of interlaminar shear strength between SMA and CFRP laminates. Some surface treatments were investigated for the improvement of bonding property by peel resistance test and single lap shear strength test. The second phase is the investigation of fabrication technique for producing a CFRP panel with embedded SMA foils. Fixture jigs were devised to introduce tensile loads during the fabrication process. The third phase is the strength demonstration of CFRP laminates with embedded SMA foils. Some strength test were conducted to obtain the design data for aircraft structures. It is confirmed that the shrinking force of pre-strained SMA influences to the strength and the crack density of CFRP panel.

Physiological responses to low atmospheric pressure stunning and the implications for welfare.

PubMed

McKeegan, D E F; Sandercock, D A; Gerritzen, M A

2013-04-01

In low atmospheric pressure stunning (LAPS), poultry are rendered unconscious before slaughter by gradually reducing oxygen tension in the atmosphere to achieve a progressive anoxia. The effects of LAPS are not instantaneous, so there are legitimate welfare concerns around the experience of birds before loss of consciousness. Using self-contained telemetry logging units, high-quality continuous electroencephalogram (EEG) and electrocardiogram (EKG) recordings were obtained from 28 broiler chickens during exposure to LAPS in a commercial poultry processing plant. Application of LAPS was associated with changes in the EEG pattern in the form of increases in total power, decreases in mean frequency, and in particular, increases in slow-wave (delta) activity, indicating a gradual loss of consciousness. Increased delta wave activity was seen within 10 s of LAPS onset and consistently thereafter, peaking at 30 s into LAPS at which point the EEG signal shared characteristics with that of birds in a surgical plane of anesthesia. During LAPS, heart rate consistently decreased, with more pronounced bradycardia and arrhythmia observed after 30 s. No heart rate increases were observed in the period when the birds were potentially conscious. After an initial quiescent period, brief body movements (presumed to be ataxia/loss of posture) were seen on average at 39 s into the LAPS process. Later (after 120 s on average), artifacts related to clonic (wing flapping) and tonic (muscle spasms) convulsions were observed in the EKG recordings. Based on EEG analysis and body movement responses, a conservative estimate of time to loss of consciousness is approximately 40 s. The lack of behavioral responses indicating aversion or escape and absence of heart rate elevation in the conscious period strongly suggest that birds do not find LAPS induction distressing. Collectively, the results suggest that LAPS is a humane approach that has the potential to improve the welfare of poultry at slaughter by gradually inducing unconsciousness without distress, eliminating live shackling and ensuring every bird is adequately stunned before exansguination.

[Seatbelts on school buses: are they safe for our children?].

PubMed

Peleg, Kobi; Goldman, Sharon

2008-01-01

In Israel, 280,000 pupils travel daily to school and back home by means of school transportation. In an effort to increase school transportation safety, the installation of lap belts in school transportation vehicles is required since September 1, 2006. In Israel, laws are often passed with good intentions, but frequently without exploring the potential outcomes. Traffic regulation 364a states that "lap belts or other seatbelts" are required in all vehicles used for school transportation. The objective of this study is to review the world-wide literature regarding seatbelts on school buses with an emphasis to identify the risks associated with lap restraints. Over 50 studies, articles and position papers referring to the efficacy of seatbelts, with an emphasis on school transportation, were reviewed. According to the literature, this new traffic regulation could produce more devastating outcomes than previously. Seatbelts were designed to prevent passenger injuries during a motor vehicle crash. Researchers have not proven efficacy of seatbelts in school buses. Lap-only belts have shown to increase the risk of severe injury among children, even in mild crashes. Since young children are not adequately developed to take the force of a lap-only restraint, these belts have been associated with internal injuries, lumbar fracture-dislocations, abdominal contusions and head injuries. The National Highway Traffic Safety Administration (NHTSA) in the USA has reported that lap belts are not effective in preventing injury among children traveling by bus. Children have a two to three fold risk of severe injury when using lap only restraints. On the contrary, lap-shoulder belts may reduce the risk of abdominal injuries by up to 50% relative to lap-only belts. In conclusion, policy makers aimed to implement regulations which will reduce injuries and fatalities. However, neglecting to carry out an in-depth professional review has brought about a regulation requiring lap belts in school buses; a decision which, according to international research studies, can potentially increase the injury risks among pupils.

Theoretical and Experimental Evaluation of the Bond Strength Under Peeling Loads

NASA Technical Reports Server (NTRS)

Nayeb-Hashemi, Hamid; Jawad, Oussama Cherkaoui

1997-01-01

Reliable applications of adhesively bonded joints require understanding of the stress distribution along the bond-line and the stresses that are responsible for the joint failure. To properly evaluate factors affecting peel strength, effects of defects such as voids on the stress distribution in the overlap region must be understood. In this work, the peel stress distribution in a single lap joint is derived using a strength of materials approach. The bonded joint is modeled as Euler-Bernoulli beams, bonded together with an adhesive. which is modeled as an elastic foundation which can resist both peel and shear stresses. It is found that for certain adhesive and adherend geometries and properties, a central void with the size up to 50 percent of the overlap length has negligible effect on the peak peel and shear stresses. To verify the solutions obtained from the model, the problem is solved again by using the finite element method and by treating the adherends and the adhesive as elastic materials. It is found that the model used in the analysis not only predicts the correct trend for the peel stress distribution but also gives rather surprisingly close results to that of the finite element analysis. It is also found that both shear and peel stresses can be responsible for the joint performance and when a void is introduced, both of these stresses can contribute to the joint failure as the void size increases. Acoustic emission (AE) activities of aluminum-adhesive-aluminum specimens with different void sizes were monitored. The AE ringdown counts and energy were very sensitive and decreased significantly with the void size. It was observed that the AE events were shifting towards the edge of the overlap where the maximum peeling and shearing stresses were occurring as the void size increased.

Lipid accumulation product and insulin resistance in Iranian PCOS prevalence study.

PubMed

Hosseinpanah, Farhad; Barzin, Maryam; Erfani, Hadi; Serahati, Sara; Ramezani Tehrani, Fahimeh; Azizi, Fereidoun

2014-07-01

To evaluate diagnostic accuracy of lipid accumulation product (LAP) index as a marker of insulin resistance in a community-based population with polycystic ovary syndrome (PCOS), compared with healthy women. Anthropometric measurements, biochemical parameters, LAP index and insulin resistance (IR) were compared in 134 PCOS subjects and 414 healthy women recruited from 1126 reproductive aged women (18-45 years), participants of the Iranian PCOS Prevalence Study. LAP was defined as [WC (cm)-58] ×TG (mmol/l)]. PCOS was diagnosed using the Rotterdam criteria, and IR was defined using the homeostatic model assessment IR. LAP, body mass index (BMI), waist circumference (WC) and waist-to-hip ratio (WHR) were compared using the two-tailed Spearman rank correlation test and analysing the receiver operating characteristic (ROC) curves for IR. Among the PCOS subjects, the mean ± SD age, BMI, WC and WHR were 32·2 ± 7·7 years, 26·8 ± 5·8 kg/m(2) , 85·2 ± 13·2 cm and 0·80 ± 0·06, respectively, and the median (IQ25-75) of LAP index was 34·03 (17·8-66·3). There was significant correlation between HOMA-IR index and LAP in patients with PCOS (r = 0·41; P < 0·001). Also, ROC curves analysis revealed that the optimal cut-off value for LAP to define the presence of IR was 34·1 (sensitivity: 75%; specificity: 58%). LAP showed the highest area under curve (AUC) (P < 0·001). Among PCOS subjects, LAP index has the strongest diagnostic accuracy for detection of IR in comparison with BMI, WC and WHR. © 2013 John Wiley & Sons Ltd.

Noncanonical autophagy inhibits the auto-inflammatory, lupus-like response to dying cells

PubMed Central

Martinez, Jennifer; Cunha, Larissa D.; Park, Sunmin; Yang, Mao; Lu, Qun; Orchard, Robert; Li, Quan-Zhen; Yan, Mei; Janke, Laura; Guy, Cliff; Linkermann, Andreas; Virgin, Herbert W.; Green, Douglas R.

2016-01-01

Defects in dying cell clearance are postulated to underlie the pathogenesis of systemic lupus erythematosus (SLE)1. Mice lacking molecules associated with dying cell clearance develop SLE-like disease2, and phagocytes from SLE patients often display defective clearance and increased inflammatory cytokine production when exposed to dying cells in vitro. Previously, we3–6 and others7 described a form of noncanonical autophagy called “LC3-associated phagocytosis” (LAP), wherein phagosomes containing engulfed particles, including dying cells3,4,7, recruit elements of the autophagy pathway to facilitate phagosome maturation and digestion of cargo. Genome-wide association studies have identified polymorphisms in atg58 and possibly atg79, involved in both canonical autophagy and LAP3–7, as predisposition markers for SLE. Here, we describe the consequences of defective LAP in vivo. Mice lacking any of several components of the LAP pathway display elevated serum inflammatory cytokines, autoantibodies, glomerular immune complex deposition, and evidence of kidney damage. Dying cells, injected into LAP-deficient animals, are engulfed but not efficiently degraded, and trigger acute elevation of pro-inflammatory cytokines but not the anti-inflammatory interleukin (IL)-10. Repeated injection of dying cells into LAP-deficient, but not LAP-sufficient animals accelerated SLE-like disease, including increased serum levels of autoantibodies. In contrast, animals deficient for genes required for canonical autophagy but not LAP do not display defective dead cell clearance, inflammatory cytokine production, or SLE-like disease, and like wild-type animals, produce IL-10 in response to dying cells. Therefore, defects in LAP, rather than canonical autophagy, can cause SLE-like phenomena, and may contribute to the pathogenesis of SLE. PMID:27096368

Synergistic Effect of Ionizing Radiation and β-lapachone against RKO Human Colon Adenocarcinoma Cells

PubMed Central

Kim, Eun Jung; Ji, In-Mi; Ahn, Ki-Jung; Choi, Eun Kyung; Park, Heon-Jin; Lim, Byung Uk; Song, Chang W.

2005-01-01

Purpose To reveal the interaction between β-Lapachone (β-lap) and ionizing radiation in causing cell death in RKO human colon adenocarcinoma cells, and to elucidate the potential usefulness of combined β-lap treatment and radiotherapy for cancer treatment. Materials and Methods The cytotoxicities of various treatments were determined in vitro using clonogenic and apoptotic cell death. The changes in cell cycle distribution were studied using flow cytometry and an in vitro kinase assay. The tumor growth was studied using RKO tumors grown s.c. in the hind leg BALB/c- nuslc nude mice. Results β-lap caused clonogenic cell death and rapid apoptosis in RKO cells in vitro, in a dose dependent manner. The repair of sublethal radiation damage was almost completely inhibited when cells were maintained in β-lap during the interval between the two-dose irradiation. Flow cytometry study demonstrated that β-lap induced apoptosis, independent of the cell cycle phase, and completely prohibited the induction of radiation-induced G2 arrest in irradiated cells. The prohibition of radiation-induced G2 arrest is unclear, but may be related to the profound suppression of the p53, p21 and cyclin B1-Cdc2 kinase activities observed in cells treated with β-lap. The combination of β-lap and radiation markedly enhanced the radiation-induced growth suppression of tumors. Conclusion β-lap is cytotoxic against RKO cells, both in vitro and in vivo, and also sensitized cells to ionizing radiation by inhibiting sublethal radiation damage repair. β-lap is potentially useful as a potent anti-cancer chemotherapy drug and potent radiosensitizer against caner cells. PMID:19956501

Flexible cryogenic conduit

DOEpatents

Brindza, Paul Daniel; Wines, Robin Renee; Takacs, James Joseph

1999-01-01

A flexible and relatively low cost cryogenic conduit is described. The flexible cryogenic conduit of the present invention comprises a first inner corrugated tube with single braided serving, a second outer corrugated tube with single braided serving concentric with the inner corrugated tube, and arranged outwardly about the periphery of the inner corrugated tube and between the inner and outer corrugated tubes: a superinsulation layer; a one half lap layer of polyester ribbon; a one half lap layer of copper ribbon; a spirally wound refrigeration tube; a second one half lap layer of copper ribbon; a second one half lap layer of polyester ribbon; a second superinsulation layer; a third one half lap layer of polyester ribbon; and a spirally wound stretchable and compressible filament.

Single crystal diamond lapping procedure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grayson, R.A.

A facility capable of resharpening quality cutting edges on single crystal diamond cutting tools was needed as the demand in precision machining of special optical surfaces became a common occurrence here at Lawrence Livermore National Laboratory. A specially constructed lapping machine using an air bearing spindle was built to achieve the required edge quality. The basic design for this lap was taken out of a technical report by W.L. Duke and R.T. Lovell of Oak Ridge Y-12 Plant Union Carbide Corp. We have also purchased two commercially built lapping machines recommended to us by Mr. Cory A. Knottenbelt, formerly ofmore » R.C.A. Diamond Lapping Facility, in Indianapolis, Indiana, now doing state-of-the-art polishing and relapping at LLNL facilities.« less

The feasibility of producing aluminum-lithium structures for cryogenic tankage applications by laser beam welding

NASA Technical Reports Server (NTRS)

Martukanitz, R. P.; Lysher, K. G.

1993-01-01

Aluminum-lithium alloys exhibit high strength, high elastic modulus, and low density as well as excellent cryogenic mechanical properties making them ideal material candidates for cryogenic tanks. NASA has proposed the use of 'built-up' structure for panels fabricated into cryogenic tanks replacing current conventional machining. Superplastically formed stiffeners would be joined to sheet (tank skin) that had been roll formed to the radius of the tank in order to produce panels. Aluminum-lithium alloys of interest for producing the built-up structure include alloy 2095-T6 stiffeners to 2095-T8 sheet and alloy 8090-T6 stiffeners to 2090-T83 sheet. Laser welding, with comparable joint properties, offers the following advantages over conventional welding: higher production rates, minimal degradation within the heat affected zones, and full process automation. This study established process parameters for laser beam welding, mechanical property determinations, metallographic characterization, and fabrication of prototype panels. Tensile tests representing partial penetration of the skin alloys provided joint efficiencies between 65 and 77 percent, depending upon alloy and degree of penetration. Results of tension shear tests of lap welds indicated that the combination of 2095-T6 to 2090-T8 exhibited significantly higher weld shear strength at the interface in comparison to welds of 8090-T6 to 2090-T83. The increased shear strength associated with 2095 is believed to be due to the alloy's ability to precipitation strengthening (naturally age) after welding.

Enhanced tendon-to-bone repair through adhesive films.

PubMed

Linderman, Stephen W; Golman, Mikhail; Gardner, Thomas R; Birman, Victor; Levine, William N; Genin, Guy M; Thomopoulos, Stavros

2018-04-01

Tendon-to-bone surgical repairs have unacceptably high failure rates, possibly due to their inability to recreate the load transfer mechanisms of the native enthesis. Instead of distributing load across a wide attachment footprint area, surgical repairs concentrate shear stress on a small number of suture anchor points. This motivates development of technologies that distribute shear stresses away from suture anchors and across the enthesis footprint. Here, we present predictions and proof-of-concept experiments showing that mechanically-optimized adhesive films can mimic the natural load transfer mechanisms of the healthy attachment and increase the load tolerance of a repair. Mechanical optimization, based upon a shear lag model corroborated by a finite element analysis, revealed that adhesives with relatively high strength and low stiffness can, theoretically, strengthen tendon-to-bone repairs by over 10-fold. Lap shear testing using tendon and bone planks validated the mechanical models for a range of adhesive stiffnesses and strengths. Ex vivo human supraspinatus repairs of cadaveric tissues using multipartite adhesives showed substantial increase in strength. Results suggest that adhesive-enhanced repair can improve repair strength, and motivate a search for optimal adhesives. Current surgical techniques for tendon-to-bone repair have unacceptably high failure rates, indicating that the initial repair strength is insufficient to prevent gapping or rupture. In the rotator cuff, repair techniques apply compression over the repair interface to achieve contact healing between tendon and bone, but transfer almost all force in shear across only a few points where sutures puncture the tendon. Therefore, we evaluated the ability of an adhesive film, implanted between tendon and bone, to enhance repair strength and minimize the likelihood of rupture. Mechanical models demonstrated that optimally designed adhesives would improve repair strength by over 10-fold. Experiments using idealized and clinically-relevant repairs validated these models. This work demonstrates an opportunity to dramatically improve tendon-to-bone repair strength using adhesive films with appropriate material properties. Copyright © 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Peritoneal drainage does not stabilize extremely low birth weight infants with perforated bowel: data from the NET Trial.

PubMed

Rees, Clare M; Eaton, Simon; Khoo, A Kate; Kiely, Edward M; Pierro, Agostino

2010-02-01

Proponents of peritoneal drainage (PD) hypothesize that it allows stabilization before laparotomy. We examined this hypothesis by comparing clinical status before and after either PD or primary laparotomy (LAP). In an ethically approved, international, prospective randomized controlled trial (2002-2006), extremely low birth weight (<1000 g) infants with pneumoperitoneum received primary PD (n = 35) or LAP (n = 34). Physiologic data were collected prospectively and organ failure scores calculated and compared between preprocedure and day 1 after procedure. Data, expressed as mean +/- SD or median (range), were analyzed using appropriate statistical tests. There was no postprocedure improvement in either PD or LAP group comparing heart rate (PD, P = 1.0; LAP, P = .6), blood pressure (PD, P = .6; LAP, P = .8), inotrope requirement (PD, P = .2; LAP, P = .3), or Arterial partial pressure of oxygen/fraction of inspired oxygen ratio (PD, P = .1; LAP, P = .5). Infants managed with PD had a worsening cardiovascular status (P = .05). There were no differences in total organ failure score in either group (PD, P = .5; LAP, P = 1). Only 4 infants survived with PD alone with no difference between preprocedure and postprocedure organ failure score (P = .4). Peritoneal drainage does not immediately improve clinical status in extremely low birth weight infants with bowel perforation. The use of PD as a stabilizing or temporizing measure is not supported by these results. Copyright 2010 Elsevier Inc. All rights reserved.

Leucine aminopeptidase, HlLAP, from the ixodid tick Haemaphysalis longicornis, plays vital roles in the development of oocytes.

PubMed

Hatta, Takeshi; Tsuji, Naotoshi; Miyoshi, Takeharu; Islam, M Khyrul; Alim, M Abdul; Yamaji, Kayoko; Anisuzzaman; Fujisaki, Kozo

2010-06-01

Female ixodid ticks are amazing invertebrate animals which efficiently convert a large amount of nutrients derived from their ingested blood meals into eggs. Although oocyte development (vitellogenesis) in ticks is triggered by a blood meal and is assumed to be supported by nutrition derived from ovarian cells connecting oocytes, little is known about the ovarian molecules processing nutrient materials for the vitellogenesis. In this study, we have suggested a putative function of leucine aminopeptidase (HlLAP) in the ovary of parthenogenetic adult ixodid tick Haemaphysalis longicornis regarding a negative output of reproduction following disruption of HlLAP gene by RNA interference. Endogenous HlLAP was shown to be localized in the ovarian cells, including ovarian epithelial and pedicel cells which were assumed to provide nutrients for the developing oocytes. Histological studies demonstrated that a majority of immature oocytes in HlLAP gene knockdown ticks were transformed into abnormal morpho-histological oocytes with vacuolated cytoplasm and/or condensed nucleus. Taken together, a reduction of the numbers of laid eggs in the HlLAP gene knockdown ticks may be due to the degeneration of immature oocytes following deprivation of nutrients such as amino acids supplied not only by midgut HlLAP but also by the ovarian HlLAP. Regulation of the tick molecules involved in nutrient metabolism for the reproduction, including blood digestion and vitellogenesis, would help in controlling the tick population and tick-borne pathogens.

Estimating frame bulk and shear moduli of two double porosity layers by ultrasound transmission.

PubMed

Bai, Ruonan; Tinel, Alain; Alem, Abdellah; Franklin, Hervé; Wang, Huaqing

2016-08-01

The acoustic plane wave transmission by water saturated double porosity media is investigated. Two samples of double porosity media assumed to obey Berryman and Wang (BW) extension (Berryman and Wang, 1995, 2000) of Biot's theory in the low frequency regime are under consideration: ROBU® (pure binder-free borosilicate glass 3.3 manufactured to form the individual grains) and Tobermorite 11Å (the individual porous cement grains show irregular shapes). The de facto gap existing between theoretical and experimental data can be minimized by modifying adequately two of the parameters estimated from triaxial tests: the frame bulk and shear moduli. The frequency dependent imaginary parts that follow necessary from the minimization are in relation with the energy losses due to contact relaxation and friction between grains. Copyright © 2016 Elsevier B.V. All rights reserved.

Phase III, Randomized Study of Dual Human Epidermal Growth Factor Receptor 2 (HER2) Blockade With Lapatinib Plus Trastuzumab in Combination With an Aromatase Inhibitor in Postmenopausal Women With HER2-Positive, Hormone Receptor-Positive Metastatic Breast Cancer: ALTERNATIVE.

PubMed

Johnston, Stephen R D; Hegg, Roberto; Im, Seock-Ah; Park, In Hae; Burdaeva, Olga; Kurteva, Galina; Press, Michael F; Tjulandin, Sergei; Iwata, Hiroji; Simon, Sergio D; Kenny, Sarah; Sarp, Severine; Izquierdo, Miguel A; Williams, Lisa S; Gradishar, William J

2018-03-10

Purpose Human epidermal growth factor receptor 2 (HER2) targeting plus endocrine therapy (ET) improved clinical benefit in HER2-positive, hormone receptor (HR)-positive metastatic breast cancer (MBC) versus ET alone. Dual HER2 blockade enhances clinical benefit versus single HER2 blockade. The ALTERNATIVE study evaluated the efficacy and safety of dual HER2 blockade plus aromatase inhibitor (AI) in postmenopausal women with HER2-positive/HR-positive MBC who received prior ET and prior neo(adjuvant)/first-line trastuzumab (TRAS) plus chemotherapy. Methods Patients were randomly assigned (1:1:1) to receive lapatinib (LAP) + TRAS + AI, TRAS + AI, or LAP + AI. Patients for whom chemotherapy was intended were excluded. The primary end point was progression-free survival (PFS; investigator assessed) with LAP + TRAS + AI versus TRAS + AI. Secondary end points were PFS (comparison of other arms), overall survival, overall response rate, clinical benefit rate, and safety. Results Three hundred fifty-five patients were included in this analysis: LAP + TRAS + AI (n = 120), TRAS + AI (n = 117), and LAP + AI (n = 118). Baseline characteristics were balanced. The study met its primary end point; superior PFS was observed with LAP + TRAS + AI versus TRAS + AI (median PFS, 11 v 5.7 months; hazard ratio, 0.62; 95% CI, 0.45 to 0.88; P = .0064). Consistent PFS benefit was observed in predefined subgroups. Overall response rate, clinical benefit rate, and overall survival also favored LAP + TRAS + AI. The median PFS with LAP + AI versus TRAS + AI was 8.3 versus 5.7 months (hazard ratio, 0.71; 95% CI, 0.51 to 0.98; P = .0361). Common adverse events (AEs; ≥ 15%) with LAP + TRAS + AI, TRAS + AI, and LAP + AI were diarrhea (69%, 9%, and 51%, respectively), rash (36%, 2%, and 28%, respectively), nausea (22%, 9%, and 22%, respectively), and paronychia (30%, 0%, and 15%, respectively), mostly grade 1 or 2. Serious AEs were reported similarly across the three groups, and AEs leading to discontinuation were lower with LAP + TRAS + AI. Conclusion Dual HER2 blockade with LAP + TRAS + AI showed superior PFS benefit versus TRAS + AI in patients with HER2-positive/HR-positive MBC. This combination offers an effective and safe chemotherapy-sparing alternative treatment regimen for this patient population.

A Semi-Analytical Method for Determining the Energy Release Rate of Cracks in Adhesively-Bonded Single-Lap Composite Joints

NASA Technical Reports Server (NTRS)

Yang, Charles; Sun, Wenjun; Tomblin, John S.; Smeltzer, Stanley S., III

2007-01-01

A semi-analytical method for determining the strain energy release rate due to a prescribed interface crack in an adhesively-bonded, single-lap composite joint subjected to axial tension is presented. The field equations in terms of displacements within the joint are formulated by using first-order shear deformable, laminated plate theory together with kinematic relations and force equilibrium conditions. The stress distributions for the adherends and adhesive are determined after the appropriate boundary and loading conditions are applied and the equations for the field displacements are solved. Based on the adhesive stress distributions, the forces at the crack tip are obtained and the strain energy release rate of the crack is determined by using the virtual crack closure technique (VCCT). Additionally, the test specimen geometry from both the ASTM D3165 and D1002 test standards are utilized during the derivation of the field equations in order to correlate analytical models with future test results. The system of second-order differential field equations is solved to provide the adherend and adhesive stress response using the symbolic computation tool, Maple 9. Finite element analyses using J-integral as well as VCCT were performed to verify the developed analytical model. The finite element analyses were conducted using the commercial finite element analysis software ABAQUS. The results determined using the analytical method correlated well with the results from the finite element analyses.

Do Clustering Monoclonal Antibody Solutions Really Have a Concentration Dependence of Viscosity?

PubMed Central

Pathak, Jai A.; Sologuren, Rumi R.; Narwal, Rojaramani

2013-01-01

Protein solution rheology data in the biophysics literature have incompletely identified factors that govern hydrodynamics. Whereas spontaneous protein adsorption at the air/water (A/W) interface increases the apparent viscosity of surfactant-free globular protein solutions, it is demonstrated here that irreversible clusters also increase system viscosity in the zero shear limit. Solution rheology measured with double gap geometry in a stress-controlled rheometer on a surfactant-free Immunoglobulin solution demonstrated that both irreversible clusters and the A/W interface increased the apparent low shear rate viscosity. Interfacial shear rheology data showed that the A/W interface yields, i.e., shows solid-like behavior. The A/W interface contribution was smaller, yet nonnegligible, in double gap compared to cone-plate geometry. Apparent nonmonotonic composition dependence of viscosity at low shear rates due to irreversible (nonequilibrium) clusters was resolved by filtration to recover a monotonically increasing viscosity-concentration curve, as expected. Although smaller equilibrium clusters also existed, their size and effective volume fraction were unaffected by filtration, rendering their contribution to viscosity invariant. Surfactant-free antibody systems containing clusters have complex hydrodynamic response, reflecting distinct bulk and interface-adsorbed protein as well as irreversible cluster contributions. Literature models for solution viscosity lack the appropriate physics to describe the bulk shear viscosity of unstable surfactant-free antibody solutions. PMID:23442970

The Effect of Symmetry on the Hydrodynamic Stability of and Bifurcation from Planar Shear Flows

DTIC Science & Technology

1990-12-01

Effect of Symmetry on the Hydrodynamic Stability of ant Bifurcation from Planar Shear Flows AFOSR-88-0196 6. AUTHOR(S) 61102F 2304/A4 Thomas J. Bridges 7...December 1990 The Effect of Symmetry on the Hydrodynamic Stability of and Bifurcation from Planar Shear Flows TIIhOMAS J. BIUDGES MATl EM ATIc(AL...spatial stabili’.y into the nonlinear regime and a theory for spa- tial Hopf bifurcation , spatial Floquet theory, wavelength doubling and spatially quasi

Development of Microstructure and Crystallographic Texture in a Double-Sided Friction Stir Welded Microalloyed Steel

NASA Astrophysics Data System (ADS)

Rahimi, S.; Wynne, B. P.; Baker, T. N.

2017-01-01

The evolution of microstructure and crystallographic texture has been investigated in double-sided friction stir welded microalloyed steel, using electron backscatter diffraction (EBSD). The microstructure analyses show that the center of stirred zone reached a temperature between Ac1 and Ac3 during FSW, resulting in a dual-phase austenitic/ ferritic microstructure. The temperatures in the thermo-mechanically affected zone and the overlapped area between the first and second weld pass did not exceed the Ac1. The shear generated by the rotation probe occurs in austenitic/ferritic phase field where the austenite portion of the microstructure is transformed to a bainitic ferrite, on cooling. Analysis of crystallographic textures with regard to shear flow lines generated by the probe tool shows the dominance of simple shear components across the whole weld. The austenite texture at Ac1 - Ac3 is dominated by the B { {1bar{1}2} }< 110rangle and bar{B} { {bar{1}1bar{2}} }< bar{1}bar{1}0rangle simple shear texture components, where the bainite phase textures formed on cooling were inherited from the shear textures of the austenite phase with relatively strong variant selection. The ferrite portion of the stirred zone and the ferrites in the thermo-mechanically affected zones and the overlapped area underwent shear deformation with textures dominated by the D1 { {bar{1}bar{1}2} }< 111rangle and D2 { {11bar{2}} }< 111rangle simple shear texture components. The formation of ultrafine equiaxed ferrite with submicron grain size has been observed in the overlapped area between the first and second weld pass. This is due to continuous dynamic strain-induced recrystallization as a result of simultaneous severe shear deformation and drastic undercooling.

A lapping apparatus for hard tissue sections.

PubMed

Malcolm, A S

1975-02-01

A Lapping Apparatus is described which enables sections both embedded and unembedded to be ground plano parallel within +/- 1 micron. Sections cemented to steel subplates are retained by a magnetic holder which simplifies loading and unloading. Good surface and edge finish can be obtained and only short lapping times are required.

Machine imparting complex rotary motion for lapping a spherical inner diameter

DOEpatents

Carroll, Thomas A.; Yetter, Harold H.

1986-01-01

An apparatus for imparting complex rotary motion is used to lap an inner spherical diameter surface of a workpiece. A lapping tool consists of a dome and rod mounted along the dome's vertical axis. The workpiece containing the lapping tool is held in a gimbal which uses power derived from a secondary takeoff means to impart rotary motion about a horizontal axis. The gimbal is rotated about a vertical axis by a take means while mounted at a radially outward position on a rotating arm.

Machine imparting complex rotary motion for lapping a spherical inner diameter

DOEpatents

Carroll, T.A.; Yetter, H.H.

1985-01-30

An apparatus for imparting complex rotary motion is used to lap an inner spherical diameter surface of a workpiece. A lapping tool consists of a dome and rod mounted along the dome's vertical axis. The workpiece containing the lapping tool is held in a gimbal which uses power derived from a secondary takeoff means to impart rotary motion about a horizontal axis. The gimbal is rotated about a vertical axis by a take means while mounted at a radially outward position on a rotating arm.

Computer-Controlled Cylindrical Polishing Process for Development of Grazing Incidence Optics for Hard X-Ray Region

NASA Technical Reports Server (NTRS)

Khan, Gufran Sayeed; Gubarev, Mikhail; Speegle, Chet; Ramsey, Brian

2010-01-01

The presentation includes grazing incidence X-ray optics, motivation and challenges, mid spatial frequency generation in cylindrical polishing, design considerations for polishing lap, simulation studies and experimental results, future scope, and summary. Topics include current status of replication optics technology, cylindrical polishing process using large size polishing lap, non-conformance of polishin lap to the optics, development of software and polishing machine, deterministic prediction of polishing, polishing experiment under optimum conditions, and polishing experiment based on known error profile. Future plans include determination of non-uniformity in the polishing lap compliance, development of a polishing sequence based on a known error profile of the specimen, software for generating a mandrel polishing sequence, design an development of a flexible polishing lap, and computer controlled localized polishing process.

Evolution and Growth Competition of Salt Fingers in Saline Lake with Slight Wind Shear

NASA Astrophysics Data System (ADS)

Yang, Ray-Yeng; Hwung, Hwung-Hweng; Shugan, Igor

2010-05-01

Since the discover of double-diffusive convection by Stommel, Arons & Blanchard (1956), 'evidence has accumulated for the widespread presence of double-diffusion throughout the ocean' and for its 'significant effects on global water-mass structure and the thermohaline convection' (Schmitt, 1998). The salt-fingering form of double-diffusion has particularly attracted interest because of salt-finger convection being now widely recognized as an important mechanism for mixing heat and salt both vertically and laterally in the ocean and saline lake. In oceanographic situations or saline lake where salt fingers may be an important mechanism for the transport of heat and salt in the vertical, velocity shears may also be present. Salt finger convection is analogous to Bénard convection in that the kinetic energy of the motions is obtained from the potential energy stored in the unstable distribution of a stratifying component. On the basis of the thermal analogy it is of interest to discover whether salt fingers are converted into two-dimensional sheets by the wind shear, and how the vertical fluxes of heat and salt are changed by the wind shear. Salt finger convection under the effect of steady wind shear is theoretically examined in this paper. The evolution of developing in the presence of a vertical density gradient disturbance and the horizontal Couette flow is considered near the onset of salt fingers in the saline lake under a moderate rate of wind shear. We use velocity as the basic variable and solve the pressure Poisson equation in terms of the associated Green function. Growth competition between the longitudinal rolls (LR) and the transverse rolls (TR), whose axes are respectively in the direction parallel to and perpendicular to the Couette flow, is investigated by the weakly nonlinear analysis of coupled-mode equations. The results show that the TR mode is characterized in some range of the effective Rayleigh number, and that the stability is dominated by the LR mode in the system. KEY WORDS: evolution, saline lake, salt finger convection, wind shear, growth competition, longitudinal rolls, transverse rolls, coupled-mode equations.

The behavior of bonded doubler splices for composite sandwich panels

NASA Technical Reports Server (NTRS)

Zeller, T. A.; Weisahaar, T. A.

1980-01-01

The results of an investigation into the behavior of adhesively bonded doubler splices of two composite material sandwich panels are presented. The splices are studied from three approaches: analytical; numerical (finite elements); and experimental. Several parameters that characterize the splice are developed to determine their influence upon joint strength. These parameters are: doubler overlap length; core stiffness; laminate bending stiffness; the size of the gap between the spliced sandwich panels; and room and elevated temperatures. Similarities and contrasts between these splices and the physically similar single and double lap joints are discussed. The results of this investigation suggest several possible approaches to improving the strength of the sandwich splices.

Effect of surface treatments on shear bond strength of denture teeth to denture base resins

PubMed Central

Bahrani, Farideh; Khaledi, Amir Ali Reza

2014-01-01

Background: Debonding of denture teeth from denture bases is the most common failure in removable dentures. The purpose of this study was to evaluate the effect of surface treatments on shear bond strength of denture teeth to heat-polymerized and autopolymerized denture base resins. Materials and Methods: In this experimental in vitro study, 60 maxillary central incisor acrylic teeth were divided into two groups. Group M was polymerized with heat-polymerized acrylic resin (Meliodent) by compression molding technique and group F was processed by autopolymerized acrylic resin (Futura Gen) by injection molding technique. Within each group, specimens were divided into three subgroups according to the teeth surface treatments (n = 10): (1) ground surface as the control group (M1 and F1), (2) ground surface combined with monomer application (M2 and F2), and (3) airborne particle abrasion by 50 μm Al2O3 (M3 and F3). The shear bond strengths of the specimens were tested by universal testing machine with crosshead speed of 5 mm/min. Data were analyzed by two-way analysis of variance (ANOVA) and Tukey's honestly significant difference (HSD) tests (P < 0.05). Results: The mean shear bond strengths of the studied groups were 96.40 ± 14.01, 124.70 ± 15.64, and 118 ± 16.38 N for M1, M2, and M3 and 87.90 ± 13.48, 117 ± 13.88, and 109.70 ± 13.78 N for F1, F2, and F3, respectively. The surface treatment of the denture teeth significantly affected their shear bond strengths to the both the denture base resins (P < 0.001). However, there were no significant differences between the groups treated by monomer or airborne particle abrasion (P = 0.29). The highest percentage of failure mode was mixed in Meliodent and adhesive in Futura Gen. Conclusion: Monomer application and airborne particle abrasion of the ridge lap area of the denture teeth improved their shear bond strengths to the denture base resins regardless of the type of polymerization. PMID:24688570

Pacing Behavior and Tactical Positioning in 500- and 1000-m Short-Track Speed Skating.

PubMed

Noorbergen, Olaf S; Konings, Marco J; Micklewright, Dominic; Elferink-Gemser, Marije T; Hettinga, Florentina J

2016-09-01

To explore pacing behavior and tactical positioning during the shorter 500- and 1000-m short-track competitions. Lap times and intermediate rankings of elite 500- and 1000-m short-track-skating competitors were collected over the 2012-13 season. First, lap times were analyzed using a MANOVA, and for each lap, differences between sex, race type, final ranking, and stage of competition were determined. Second, Kendall tau-b correlations were used to assess relationships between intermediate and final rankings. In addition, intermediate rankings of the winner of each race were examined. Top-placed athletes appeared faster than bottom-placed athletes in every lap in the 500-m, while in the 1000-m no differences were found until the final 4 laps (P < .05). Correlations between intermediate and final rankings were already high at the beginning stages of the 50-m (lap 1: r = .59) but not for the 1000-m (lap 1: r = .21). Although 500- and 1000-m short-track races are both relatively short, fundamental differences in pacing behavior and tactical positioning were found. A fast-start strategy seems to be optimal for 500-m races, while the crucial segment in 1000-m races seems to be from the 6th lap to the finish line (ie, after ± 650 m). These findings provide evidence to suggest that athletes balance between choosing an energetically optimal profile and the tactical and positional benefits that play a role when riding against an opponent, as well as contributing to developing novel insights in exploring athletic behavior when racing against opponents.

Glottic Closing Force Versus Laryngeal Adductory Pressure in the Canine Larynx.

PubMed

Paniello, Randal C; Bhatt, Neel K

2017-03-01

The strength of glottic closure with recurrent laryngeal nerve (RLN) stimulation has been indirectly measured experimentally by determining the squeezing pressure on a balloon inserted between the vocal folds, termed laryngeal adductory pressure (LAP). In this study, we sought to measure glottic closing force (GCF) directly and compare these results to LAP measures obtained with identical stimulation parameters. In canines, a method for measuring GCF was developed in which a suture was looped through a lateral thyrotomy hole, around the vocal process and back, then attached to a force gauge. The RLN was maximally stimulated and GCF recorded. The LAP was then measured as previously described, using the same stimuli. This process was repeated at 9 stimulation frequencies in 10-Hz intervals from 20 to 100 Hz. The GCF and LAP were compared using Pearson's correlation coefficient (PCC). Both sides were measured in 16 dogs, resulting in 32 data sets. The LAP measures were obtained at all frequencies, while GCF was obtained in 246 of 288 (85.4%) attempts. The maximum GCF for each dog typically occurred at 80 to 100 Hz and averaged 0.287 ± 0.106 newtons. Plotting GCF versus LAP for each hemilaryngeal preparation, the mean PCC was 0.932 ± .042 (range, 0.802-0.987). The mean PCC did not differ between control (n = 26) and postoperative (n = 6) hemilarynges. This method for measuring GCF appears valid. The high Pearson's correlation coefficient indicates strong covariance between GCF and LAP, demonstrating that they are both measures of the same physical property. The LAP is easier to perform and more consistently obtained.

Mutation in TOR1AIP1 encoding LAP1B in a form of muscular dystrophy: a novel gene related to nuclear envelopathies.

PubMed

Kayman-Kurekci, Gulsum; Talim, Beril; Korkusuz, Petek; Sayar, Nilufer; Sarioglu, Turkan; Oncel, Ibrahim; Sharafi, Parisa; Gundesli, Hulya; Balci-Hayta, Burcu; Purali, Nuhan; Serdaroglu-Oflazer, Piraye; Topaloglu, Haluk; Dincer, Pervin

2014-07-01

We performed genome-wide homozygosity mapping and mapped a novel myopathic phenotype to chromosomal region 1q25 in a consanguineous family with three affected individuals manifesting proximal and distal weakness and atrophy, rigid spine and contractures of the proximal and distal interphalangeal hand joints. Additionally, cardiomyopathy and respiratory involvement were noted. DNA sequencing of torsinA-interacting protein 1 (TOR1AIP1) gene encoding lamina-associated polypeptide 1B (LAP1B), showed a homozygous c.186delG mutation that causes a frameshift resulting in a premature stop codon (p.E62fsTer25). We observed that expression of LAP1B was absent in the patient skeletal muscle fibres. Ultrastructural examination showed intact sarcomeric organization but alterations of the nuclear envelope including nuclear fragmentation, chromatin bleb formation and naked chromatin. LAP1B is a type-2 integral membrane protein localized in the inner nuclear membrane that binds to both A- and B-type lamins, and is involved in the regulation of torsinA ATPase. Interestingly, luminal domain-like LAP1 (LULL1)-an endoplasmic reticulum-localized partner of torsinA-was overexpressed in the patient's muscle in the absence of LAP1B. Therefore, the findings suggest that LAP1 and LULL1 might have a compensatory effect on each other. This study expands the spectrum of genes associated with nuclear envelopathies and highlights the critical function for LAP1B in striated muscle. Copyright © 2014 Elsevier B.V. All rights reserved.

Modeling parameters that characterize pacing of elite female 800-m freestyle swimmers.

PubMed

Lipińska, Patrycja; Allen, Sian V; Hopkins, Will G

2016-01-01

Pacing offers a potential avenue for enhancement of endurance performance. We report here a novel method for characterizing pacing in 800-m freestyle swimming. Websites provided 50-m lap and race times for 192 swims of 20 elite female swimmers between 2000 and 2013. Pacing for each swim was characterized with five parameters derived from a linear model: linear and quadratic coefficients for effect of lap number, reductions from predicted time for first and last laps, and lap-time variability (standard error of the estimate). Race-to-race consistency of the parameters was expressed as intraclass correlation coefficients (ICCs). The average swim was a shallow negative quadratic with slowest time in the eleventh lap. First and last laps were faster by 6.4% and 3.6%, and lap-time variability was ±0.64%. Consistency between swimmers ranged from low-moderate for the linear and quadratic parameters (ICC = 0.29 and 0.36) to high for the last-lap parameter (ICC = 0.62), while consistency for race time was very high (ICC = 0.80). Only ~15% of swimmers had enough swims (~15 or more) to provide reasonable evidence of optimum parameter values in plots of race time vs. each parameter. The modest consistency of most of the pacing parameters and lack of relationships between parameters and performance suggest that swimmers usually compensated for changes in one parameter with changes in another. In conclusion, pacing in 800-m elite female swimmers can be characterized with five parameters, but identifying an optimal pacing profile is generally impractical.

Customization of laparoscopic gastric devascularization and splenectomy for gastric varices based on CT vascular anatomy.

PubMed

Kawanaka, Hirofumi; Akahoshi, Tomohiko; Nagao, Yoshihiro; Kinjo, Nao; Yoshida, Daisuke; Matsumoto, Yoshihiro; Harimoto, Norifumi; Itoh, Shinji; Yoshizumi, Tomoharu; Maehara, Yoshihiko

2018-01-01

Laparoscopic gastric devascularization(Lap GDS) and splenectomy (SPL) for gastric varices is technically challenging because of highly developed collateral vessels and bleeding tendency. We investigated the feasibility of customization of Lap GDS and SPL based on CT vascular anatomy. We analyzed 61 cirrhotic patients with gastric varices who underwent Lap GDS and SPL between 2006 and 2014. Lap GDS was customized according to the afferent feeding veins (left gastric vein (LGV) and/or posterior gastric vein (PGV)/short gastric vein (SGV)) and efferent drainage veins (gastrorenal shunt and/or gastrophrenic shunt, or numerous retroperitoneal veins) based on CT imaging. Thirty-four patients with efferent drainage veins suitable for balloon-occluded retrograde transvenous obliteration (B-RTO) underwent B-RTO instead of surgical GDS, with subsequent Lap SPL. Among 27 patients with gastric varices unsuitable for B-RTO, 15 patients with PGV/SGV underwent Lap GDS of the greater curvature and SPL, and 12 patients with LGV or LGV/PGV/SGV underwent Lap GDS of the greater and lesser curvature and SPL. The mean operation time was 294 min and mean blood loss was 198 g. There was no mortality or severe morbidity. Gastric varices were eradicated in all 61 patients, with no bleeding or recurrence during a mean follow-up of 55.9 months. The cumulative 3-, 5-, and 7-year survival rates were 92, 82, and 64%, respectively. Lap GDS and SPL customized based on CT vascular anatomy is a safe and effective procedure for treating gastric varices.

Effects of surface preparation on the long-term durability of adhesively bonded composite joints

NASA Astrophysics Data System (ADS)

Bardis, Jason Dante

The long-term durability of adhesively bonded composite joints is critical to modern aircraft structures, which are increasingly adopting bonding as an alternative option to mechanical fastening. The effects of the surface preparation of the adherends are critical, affecting initial strength, long-term durability, fracture toughness, and failure modes of bonded joints. In this study, several potential factors are evaluated, with focus on the following: (1) Effects of possible chemical contamination from release fabrics, release films, and peel plies during adherend cure. (2) Chemical and mechanical effects of abrasion on the fracture toughness and failure mode. (3) Characterization of paste and film adhesives. There are several standard test methods used to evaluate specimen fracture, but the majority concentrate on bonded metals and interlaminar composite fracture. Testing concentrated on mode I tests; a custom double cantilever beam specimen was devised and utilized, and two forms of a wedge crack test (traveling and static) were also used. Additionally, single lap shear tests were run to contrast the mode I tests. Non-destructive testing included X-ray photography of crack fronts, energy dispersive spectroscopy and X-ray photoelectron spectroscopy surface chemistry analyses, and scanning electron microscope imaging of prepared surfaces. All mode I test methods tended to be in agreement in the ranking of different surface preparation methods. Test results revealed that release agents deposited on adherend surfaces during their cure cycle prevented proper adhesion. While mechanical abrasion did improve their fracture toughness and lower their contamination greatly, the test values did not reach the levels of samples that were not contaminated before bonding, and the interfacial modes of failure did not always change to desirable modes.

Combined In-Plane and Through-the-Thickness Analysis for Failure Prediction of Bolted Composite Joints

NASA Technical Reports Server (NTRS)

Kradinov, V.; Madenci, E.; Ambur, D. R.

2004-01-01

Although two-dimensional methods provide accurate predictions of contact stresses and bolt load distribution in bolted composite joints with multiple bolts, they fail to capture the effect of thickness on the strength prediction. Typically, the plies close to the interface of laminates are expected to be the most highly loaded, due to bolt deformation, and they are usually the first to fail. This study presents an analysis method to account for the variation of stresses in the thickness direction by augmenting a two-dimensional analysis with a one-dimensional through the thickness analysis. The two-dimensional in-plane solution method based on the combined complex potential and variational formulation satisfies the equilibrium equations exactly, and satisfies the boundary conditions and constraints by minimizing the total potential. Under general loading conditions, this method addresses multiple bolt configurations without requiring symmetry conditions while accounting for the contact phenomenon and the interaction among the bolts explicitly. The through-the-thickness analysis is based on the model utilizing a beam on an elastic foundation. The bolt, represented as a short beam while accounting for bending and shear deformations, rests on springs, where the spring coefficients represent the resistance of the composite laminate to bolt deformation. The combined in-plane and through-the-thickness analysis produces the bolt/hole displacement in the thickness direction, as well as the stress state in each ply. The initial ply failure predicted by applying the average stress criterion is followed by a simple progressive failure. Application of the model is demonstrated by considering single- and double-lap joints of metal plates bolted to composite laminates.

Constitutive Behavior of Mixed Sn-Pb/Sn-3.0Ag-0.5Cu Solder Alloys

NASA Astrophysics Data System (ADS)

Tucker, J. P.; Chan, D. K.; Subbarayan, G.; Handwerker, C. A.

2012-03-01

During the transition from Pb-containing solders to Pb-free solders, joints composed of a mixture of Sn-Pb and Sn-Ag-Cu often result from either mixed assemblies or rework. Comprehensive characterization of the mechanical behavior of these mixed solder alloys resulting in a deformationally complete constitutive description is necessary to predict failure of mixed alloy solder joints. Three alloys with 1 wt.%, 5 wt.%, and 20 wt.% Pb were selected so as to represent reasonable ranges of Pb contamination expected from different 63Sn-37Pb components mixed with Sn-3.0Ag-0.5Cu. Creep and displacement-controlled tests were performed on specially designed assemblies at temperatures of 25°C, 75°C, and 125°C using a double lap shear test setup that ensures a nearly homogeneous state of plastic strain at the joint interface. The observed changes in creep and tensile behavior with Pb additions were related to phase equilibria and microstructure differences observed through differential scanning calorimetric and scanning electron microscopic cross-sectional analysis. As Pb content increased, the steady-state creep strain rates increased, and primary creep decreased. Even 1 wt.% Pb addition was sufficient to induce substantially large creep strains relative to the Sn-3.0Ag-0.5Cu alloy. We describe rate-dependent constitutive models for Pb-contaminated Sn-Ag-Cu solder alloys, ranging from the traditional time-hardening creep model to the viscoplastic Anand model. We illustrate the utility of these constitutive models by examining the inelastic response of a chip-scale package (CSP) under thermomechanical loading through finite-element analysis. The models predict that, as Pb content increases, total inelastic dissipation decreases.

A Biomimetic Structural Health Monitoring Approach Using Carbon Nanotubes

NASA Astrophysics Data System (ADS)

Liu, Yingtao; Rajadas, Abhishek; Chattopadhyay, Aditi

2012-07-01

A self-sensing nanocomposite material has been developed to track the presence of damage in complex composite structures. Multiwalled carbon nanotubes are integrated with polymer matrix to develop a novel bonding material with sensing capabilities. The changes of the piezoresistance in the presence of damage are used to monitor the condition of bonded joints, where the usual bonding material is replaced by the self-sensing nanocomposite. The feasibility of this concept is investigated through experiments conducted on single-lap joints subject to monotonic tensile loading conditions. The results show that the self-sensing nanocomposite is sensitive to crack propagation within the matrix material. An acoustic emission-based sensing technique has been used to validate these results and shows good correlation with damage growth. A digital image correlation system is used to measure the shear strain field in the joint area.

Development of high temperature service polyimide based adhesives for titanium and composite bonding applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mayhew, R.T.; Kohli, D.K.

CYTEC Engineered Materials Inc. has developed several adhesives based on LaRC{trademark} PETI-5 Polyimide Resin for bonding titanium and composite substrates. These adhesives are intended for long term service at 177{degrees}C (350{degrees}F) and are processed at 350{degrees}C (660{degrees}F) in conventional autoclaves under 50 psi pressure. Titanium lap shears range from 48.3MPa (7000 psi) at room temperature to 31MPa (4500 psi) at 177{degrees}C (350{degrees}F). Excellent metal-to-metal peels and honeycomb bonding properties are reported for these products. In addition to film adhesive, primer and paste products have been described. Pilot line quantities of several hundred square feet of the film adhesive have beenmore » produced and sampled under the CYTEC designation FM{reg_sign} x5.« less

Mixed-mode cyclic debonding of adhesively bonded composite joints. M.S. Thesis

NASA Technical Reports Server (NTRS)

Rezaizadeh, M. A.; Mall, S.

1985-01-01

A combined experimental-analytical investigation to characterize the cyclic failure mechanism of a simple composite-to-composite bonded joint is conducted. The cracked lap shear (CLS) specimens of graphite/epoxy adherend bonded with EC-3445 adhesive are tested under combined mode 1 and 2 loading. In all specimens tested, fatigue failure occurs in the form of cyclic debonding. The cyclic debond growth rates are measured. The finite element analysis is employed to compute the mode 1, mode 2, and total strain energy release rates (i.e., GI, GII, and GT). A wide range of mixed-mode loading, i.e., GI/GII ranging from 0.03 to 0.38, is obtained. The total strain energy release rate, G sub T, appeared to be the driving parameter for cyclic debonding in the tested composite bonded system.

The application of thermodynamic and spectroscopic techniques to adhesion in the polyimide/Ti 6-4 and polyphenylquinoxaline/Ti 6-4 systems

NASA Technical Reports Server (NTRS)

Dias, S.; Wightman, J. P.

1984-01-01

The results of calorimetric measurements of Ti adherend surfaces are presented. The measurements were carried out after several chemical pretreatments and after fracture of several lap shear samples aged at high temperature. The exact composition of the Ti samples was Ti(6 percent Al-4 percent V). The adhesives used were polyimides and polyphenylquinoxalines (PPQ). Each chemical pretreatment was accompanied by a unique spectroscopic feature which was characterized by XPS, SEM, and specular reflectance infrared spectroscopy. The energetics of the interaction between primer solutions and the Ti adherend were evaluated by microcalorimetry. Changes in the structure of the surface oxide layer upon heating of the adherend were deduced from immersion temperatures of the PI and PPQ solutions. The XPS and SEM data are given is a table.

The Impact of Learning Assistance Experience on Teaching Pedagogy

ERIC Educational Resources Information Center

Trammell, Jack; Kourtidis, Joanna

2018-01-01

Many administrators in Learning Assistance Programs (LAPs) have teaching duties, or take on teaching duties at some point in their careers. This study was designed to examine the impact of LAP experience on classroom pedagogy. A pilot study was utilized first through listservs and email chains to ask that question of LAP professionals. After…

Auto Mechanics I. Learning Activity Packets (LAPs). Section A--Orientation and Safety.

ERIC Educational Resources Information Center

Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

This document contains seven learning activity packets (LAPs) that outline the study activities for the orientation and safety instructional area for an Auto Mechanics I course. The seven LAPs cover the following topics: orientation, safety, hand tools, arc welding, oxyacetylene cutting, oxyacetylene fusion welding, and oxyacetylene braze welding.…

Tool holder for preparation and inspection of a radiused edge cutting tool

DOEpatents

Asmanes, Charles

1979-01-01

A tool holding fixture is provided for removably holding a radiused edge cutting tool in a tool edge lapping apparatus. The fixture allows the operator to preset the lapping radius and angle before the tool holder is placed in the fixture and the holder may be removed from the lapping apparatus to inspect the tool and simply replaced in the fixture to continue lapping in accordance with a precise alignment without realignment of the tool relative to the lap. The tool holder includes a pair of self aligning bearings in the form of precision formed steel balls connected together by a rigid shaft. The tool is held by an arm extending from the shaft and the balls set in fixed position bearing cups and the holder is oscillated back and forth about a fixed axis of rotation to lap the tool radius by means of a reversibly driven belt-pulley arrangement coupled to the shaft between the bearings. To temporarily remove the holder, the drive belt is slipped over the rearward end of the holder and the holder is lifted out of the bearing cups.

Antibiotic eluting clay mineral (Laponite®) for wound healing application: an in vitro study.

PubMed

Ghadiri, M; Chrzanowski, W; Rohanizadeh, R

2014-11-01

Different materials in form of sponge, hydrogel and film have been developed and formulated for treating and dressing burn wounds. In this study, the potential of Laponite, a gel forming clay, in combination with an antimicrobial agent (mafenide), as a wound dressing material was tested in vitro. Laponite/mafenide (Lap/Maf) hydrogel was formulated in three different ratios of Lap/Maf 1:1, 1:2, 1:3. Laponite/mafenide/alginate (Lap/Maf/Alg) film was also formulated by combining Lap/Maf gel (1:1) with alginate. Intercalation rate of mafenide into the layers of Laponite nanoparticles and physico-chemical properties, including wound dressing characteristics of materials were studied using various analytical methods. Furthermore, the degradation of materials and the release profile of mafenide were investigated in simulated wound exudates fluid and antibacterial effectiveness of the eluted mafenide was tested on a range of bacterial species. The cytotoxicity of materials was also evaluated in skin fibroblast culture. The results showed that mafenide molecules were intercalated between the nano-sized layers of Laponite. The eluted mafenide showed active antibacterial effects against all three tested bacteria. All intercalated mafenide released from Lap/Maf 1:1 and 1:2 gel formulations and nearly 80% release from 1:3 formulation during test period. No significant difference was observed in release profile of mafenide between Lap/Maf/Alg film and Lap/Maf formulations. Wound dressing tests on Lap/Maf/Alg film showed it is a breathable dressing and has capacity to absorb wound exudates. The study showed that prepared Lap/Maf composite has the potential to be used as an antibiotic eluting gel or film for wound healing application. Additionally, Laponite has shown benefits in wound healing processes by releasing Mg(2+) ions and thereby reducing the cytotoxic effect of mafenide on fibroblast cells.

Behavioural, brain and cardiac responses to hypobaric hypoxia in broiler chickens.

PubMed

Martin, Jessica E; Christensen, Karen; Vizzier-Thaxton, Yvonne; Mitchell, Malcolm A; McKeegan, Dorothy E F

2016-09-01

A novel approach to pre-slaughter stunning of chickens has been developed in which birds are rendered unconscious by progressive hypobaric hypoxia. Termed Low Atmospheric Pressure Stunning (LAPS), this approach involves application of gradual decompression lasting 280s according to a prescribed curve. We examined responses to LAPS by recording behaviour, electroencephalogram (EEG) and electrocardiogram (ECG) in individual male chickens, and interpreted these with regard to the welfare impact of the process. We also examined the effect of two temperature adjusted pressure curves on these responses. Broiler chickens were exposed to LAPS in 30 triplets (16 and 14 triplets assigned to each pressure curve). In each triplet, one bird was instrumented for recording of EEG and ECG while the behaviour of all three birds was observed. Birds showed a consistent sequence of behaviours during LAPS (ataxia, loss of posture, clonic convulsions and motionless) which were observed in all birds. Leg paddling, tonic convulsions, slow wing flapping, mandibulation, head shaking, open bill breathing, deep inhalation, jumping and vocalisation were observed in a proportion of birds. Spectral analysis of EEG responses at 2s intervals throughout LAPS revealed progressive decreases in median frequency at the same time as corresponding progressive increases in total power, followed later by decreases in total power as all birds exhibited isoelectric EEG and died. There was a very pronounced increase in total power at 50-60s into the LAPS cycle, which corresponded to dominance of the signal by high amplitude slow waves, indicating loss of consciousness. Slow wave EEG was seen early in the LAPS process, before behavioural evidence of loss of consciousness such as ataxia and loss of posture, almost certainly due to the fact that it was completely dark in the LAPS chamber. ECG recordings showed a pronounced bradycardia (starting on average 49.6s into LAPS), often associated with arrhythmia, until around 60s into LAPS when heart rate levelled off. There was a good correlation between behavioural, EEG and cardiac measures in relation to loss of consciousness which collectively provide a loss of consciousness estimate of around 60s. There were some effects of temperature adjusted pressure curves on behavioural latencies and ECG responses, but in general responses were consistent and very similar to those reported in previous research on controlled atmosphere stunning with inert gases. The results suggest that the process is humane (slaughter without avoidable fear, anxiety, pain, suffering and distress). In particular, the maintenance of slow wave EEG patterns in the early part of LAPS (while birds are still conscious) is strongly suggestive that LAPS is non-aversive, since we would expect this to be interrupted by pain or discomfort. Copyright © 2016 Elsevier Inc. All rights reserved.

Clinical outcomes and health care utilization pre- and post-laparoscopic radiofrequency ablation of symptomatic fibroids and laparoscopic myomectomy: a randomized trial of uterine-sparing techniques (TRUST) in Canada.

PubMed

Rattray, Darrien D; Weins, Laura; Regush, Lexy C; Bowen, James M; O'Reilly, Daria; Thiel, John A

2018-01-01

The objective of this study was to compare laparoscopic ultrasound-guided radiofrequency ablation of fibroids (Lap-RFA) and laparoscopic myomectomy in terms of 1) health care utilization and 2) serious complication rates. The secondary objectives were comparison of subject responses to validated symptom and quality-of-life questionnaires. We hypothesized that Lap-RFA health care utilization and clinical outcomes would not be worse than those of laparoscopic myomectomy in the aggregate. Post-market, randomized, prospective, multicenter, longitudinal, non-inferiority interventional comparative evaluation of health care utilization and clinical outcomes in premenopausal women with symptomatic uterine fibroids who desired uterine conservation was conducted. Both procedures were planned as outpatient day surgeries. Health care resource utilization was measured during the procedure day and at 1 week, 1 and 3 months post-surgery. Symptom severity and quality of life were based on patients' responses to the Uterine Fibroid Symptom Severity and Quality-of-Life Questionnaire, EuroQol-5D-visual analog scale general health status and menstrual impact questionnaires, and time from work. Forty-five participants provided written informed consent and were enrolled (Lap-RFA, n=23; myomectomy, n=22) in Canada. Hospitalization time (primary endpoint) was 6.7±3.0 hours for the Lap-RFA group and 9.9±10.7 hours for the myomectomy group (Wilcoxon, p =0.0004). Intraoperative blood loss was lesser for Lap-RFA subjects: 25.2±21.6 versus 82.4±62.5 mL ( p =0.0002). Lap-RFA procedures took lesser time than myomectomy procedures: 70.0 versus 86.5 minutes ( p =0.018), and Lap-RFA required -34.9% (130 fewer) units of surgical equipment. At 3 months, both cohorts reported the same significant symptom severity reduction (-44.8%; p <0.0001). Lap-RFA subjects also took lesser time from work: 11.1±7.6 versus 18.5±10.6 days ( p =0.0193). One myomectomy subject was hospitalized overnight after experiencing a 20-second asystole during the procedure. One Lap-RFA subject underwent a reintervention. The combined per patient direct and indirect costs of the two procedures were comparable: Lap-RFA (CAD $5,224.96) and myomectomy (CAD $5,321.96). Compared to myomectomy, Lap-RFA is associated with significantly lesser intraoperative blood loss, shorter procedure and hospitalization times, lesser consumption/use of disposable and reusable surgery equipment, reduced health care resource utilization, and faster return to work through 3 months posttreatment. Direct and indirect costs of Lap-RFA and myomectomy are comparable.

The LapSim virtual reality simulator: promising but not yet proven.

PubMed

Fairhurst, Katherine; Strickland, Andrew; Maddern, Guy

2011-02-01

The acquisition of technical skills using surgical simulators is an area of active research and rapidly evolving technology. The LapSim is a virtual reality simulator that currently allows practice of basic laparoscopic skills and some procedures. To date, no reviews have been published with reference to a single virtual reality simulator. A PubMed search was performed using the keyword "LapSim," with further papers identified from the citations of original search articles. Use of the LapSim to develop surgical skills has yielded overall results, although inconsistencies exist. Data regarding the transferability of learned skills to the operative environment are encouraging as is the validation work, particularly the use of a combination of measured parameters to produce an overall comparative performance score. Although the LapSim currently does not have any proven significant advantages over video trainers in terms of basic skills instruction and although the results of validation studies are variable, the potential for such technology to have a huge impact on surgical training is apparent. Work to determine standardized learning curves and proficiency criteria for different levels of trainees is incomplete. Moreover, defining which performance parameters measured by the LapSim accurately determine laparoscopic skill is complex. Further technological advances will undoubtedly improve the efficacy of the LapSim, and the results of large multicenter trials are anticipated.

76 FR 59590 - Airworthiness Directives; The Boeing Company Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-27

... web lap and tear strap splices of the aft pressure bulkhead at STA 1582 due to fatigue. We are... radial web lap and tear strap splices of the aft pressure bulkhead at station (STA) 1582 due to fatigue... prompted by reports of multiple site damage cracks in the radial web lap and tear strap splices of the aft...

Associação entre lipid accumulation product (LAP) e hirsutismo na síndrome do ovário policístico.

PubMed

de Oliveira, Flávia Ribeiro; Rezende, Mariana Bicalho; Faria, Nícolas Figueiredo; Dias, Tomás Ribeiro Gonçalves; de Oliveira, Walter Carlos Santos; Rocha, Ana Luiza Lunardi; Cândido, Ana Lúcia

2016-02-01

Polycystic ovary syndrome (PCOS) is the most common endocrine metabolic disorder in women between menarche and menopause. Clinical hyperandrogenism is the most important diagnostic criterion of the syndrome, which manifests as hirsutism in 70% of cases. Hirsute carriers of PCOS have high cardiovascular risk. Lipid accumulation product (LAP) is an index for the evaluation of lipid accumulation in adults and the prediction of cardiovascular risk. The aim of this study was to evaluate the association between LAP and hirsutism in women with PCOS. This was a cross-sectional observational study of a secondary database, which included 263 patients who had visited the Hyperandrogenism Outpatient Clinic from November 2009 to July 2014. The exclusion criteria were patients without Ferriman-Gallwey index (FGI) and/or LAP data. We used the Rotterdam criteria for the diagnosis of PCOS. All patients underwent medical assessment followed by measurement and recording of anthropometric data and the laboratory tests for measurement of the following: thyroid-stimulating hormone, follicle-stimulating hormone, prolactin, total testosterone, sex hormone binding globulin, 17-α-hydroxyprogesterone (follicular phase), glycohemoglobin A1c, and basal insulin. In addition, the subjects underwent lipid profiling and oral glucose tolerance tests. Other laboratory measurements were determined according to clinical criteria. LAP and the homeostatic model assessment index (HOMA-IR) were calculated using the data obtained. We divided patients into two groups: the PCOS group with normal LAP (< 34.5) and the PCOS group with altered LAP (> 34.5) to compare the occurrence of hirsutism. For statistical analysis, we used SPSS Statistics for Windows® and Microsoft Excel programs, with descriptive (frequencies, percentages, means, and standard deviations) and comparative analyses (Student's t-test and Chi-square test). We considered relations significant when the p-value was ≤ 0.05. LAP was high in most patients (n = 177; 67.3%) and the FGI indicated that 58.5% of the patients (n = 154) had hirsutism. The analysis by LAP quartiles showed a positive correlation (p = 0.04) among patients with a high FGI and an upper quartile LAP (> 79.5) when compared with those with LAP < 29.0 (lower quartile). This study demonstrated an association between high LAP and hirsutism. The FGI could represent a simple and low-cost tool to infer an increased cardiovascular risk in women with PCOS. Thieme Publicações Ltda Rio de Janeiro, Brazil.

Conditions for double layers in the earth's magnetosphere and perhaps in other astrophysical objects

NASA Technical Reports Server (NTRS)

Lyons, L. R.

1987-01-01

It is suggested that the features which govern the formation of the double layers are: (1) the divergence of the magnetospheric electric field, (2) the ionospheric conductivity, and (3) the current-voltage characteristics of auroral magnetic field lines. Also considered are conditions in other astrophysical objects that could lead to the formation of DLs in a manner analogous to what occurs in the earth's auroral zones. It is noted that two processes can drive divergent Pedersen currents within a collisional conducting layer: (1) sheared plasma flow applied anywhere along the magnetic field lines connected to the conducting layer and (2) a neutral flow with shear within the conducting layer.

Lipid Accumulation Product (LAP) and Visceral Adiposity Index (VAI) as Markers of Insulin Resistance and Metabolic Associated Disturbances in Young Argentine Women with Polycystic Ovary Syndrome.

PubMed

Abruzzese, Giselle A; Cerrrone, Gloria E; Gamez, Juan M; Graffigna, Mabel N; Belli, Susana; Lioy, Gustavo; Mormandi, Eduardo; Otero, Patricia; Levalle, Oscar A; Motta, Alicia B

2017-01-01

Polycystic ovary syndrome (PCOS) is an endocrine disorder. PCOS women are at high risk of developing insulin resistance (IR) and cardiovascular disorders since young age. We aimed to study the reliability of lipid accumulation product (LAP) and visceral adiposity index (VAI) as markers of metabolic disturbances (MD) associated with IR in young reproductive aged PCOS patients. We also evaluated the association between LAP and VAI and the presence of hyperandrogenism. In a cross-sectional study, 110 PCOS patients and 88 control women (18-35 years old) were recruited. PCOS patients were divided into 2 groups, as hyperandrogenic and non-hyperandrogenic considering the signs of hyperandrogenism (clinical or biochemical). Anthropometric measurements were taken and blood samples collected. Metabolic and anthropometric characteristics and their association with IR and associated MD were evaluated and LAP and VAI were calculated. LAP and VAI were compared with TC/HDL-c and TG/HDL-c to define the best markers of MD in this population. Independently of the phenotype, young PCOS patients showed high IR and dyslipidemia. Both LAP and VAI showed to be more effective markers to assess MD and IR in these young women than TG/HDL-c or TC/HDL-c [cut-off values: LAP: 18.24 (sensitivity: 81.43% specificity: 73.49%), positive predictive value (PPV): 75.0%, negative predictive value (NPV): 77.27%, VAI: 2.19 (sensitivity: 81.16% specificity: 72.15% PPV: 74.65% NPV: 72.22%)]. LAP and VAI are representative markers to assess MD associated with IR in young PCOS patients. All PCOS patients, independently of their androgenic condition, showed high metabolic risk. © Georg Thieme Verlag KG Stuttgart · New York.

Left atrial pressure pattern without a-wave in sinus rhythm after cardioversion affects the outcomes after catheter ablation for atrial fibrillation.

PubMed

Kishima, Hideyuki; Mine, Takanao; Takahashi, Satoshi; Ashida, Kenki; Ishihara, Masaharu; Masuyama, Tohru

2018-04-24

The a-wave in left atrial pressure (LAP) is often not observed after cardioversion (CV). We hypothesized that repeated atrial fibrillation (AF) occurs in patients who do not show a-wave pattern after CV. We investigated the impact of "LAP pattern without a-wave" on the outcome after catheter ablation (CA) for AF. We studied 100 patients (64 males, age 66 ± 8 years, 42 with non-paroxysmal AF) who underwent CA for AF. Sustained- or induced-AF were terminated with internal CV, and LAP was measured during sinus rhythm (SR) after CV. LAP pattern without a-wave was defined as absence of a-wave (the "a-wave" was defined as a protruding part by 0.2 mmHg or more from the baseline) in LAP wave form. AF was terminated with CV in all patients. Recurrent AF was detected in 35/100 (35%) during the follow-up period (13.1 ± 7.8 month). Univariate analysis revealed higher prevalence of LAP pattern without a-wave (71 vs. 17%, P < 0.0001), larger left atrial volume, elevated E wave, and decreased deceleration time as significant variables. On multivariate analysis, LAP pattern without a-wave was only independently associated with recurrent AF (P = 0.0014, OR 9.865, 95% CI 2.327-54.861). Moreover, patients with LAP pattern without a-wave had a higher risk of recurrent AF than patients with a-wave (25/36 patients, 69 vs. 10/64 patients, 16%, log-rank P < 0.0001). Left atrial pressure pattern without a-wave in sinus rhythm after cardioversion could predict recurrence after catheter ablation for AF.

Sensory quality of broiler breast meat influenced by low atmospheric pressure stunning, deboning time and cooking methods.

PubMed

Schilling, M W; Radhakrishnan, V; Vizzier-Thaxton, Y; Christensen, K; Williams, J B; Joseph, P

2015-06-01

Stunning method (low atmospheric pressure stunning, LAPS and electrical stunning, ES), deboning time (0.75 h and 4 h), and cooking method (baking, frying, and sous vide) were evaluated for their impact on the descriptive sensory characteristics and consumer acceptability of breast meat (n=576, 144 birds per stunning × deboning time combination). Sensory evaluation was conducted by trained descriptive (n=8) and consumer (n=185) panels. On average, no differences (P>0.05) existed in the sensory acceptability of fried and sous vide cooked broiler breast treatment combinations. However, for oven-baking, the LAPS treatment that was deboned at 4 h was more acceptable (P<0.05) than other treatments and the ES and LAPS 4 h samples had greater (P<0.05) acceptability for texture than their ES and LAPS 0.75 h counterparts. Since consumers were highly variable in their liking of chicken breast treatments, consumers were grouped into clusters for each cooking method based on liking and preference. Cluster analysis data revealed that the largest groups of consumers liked (score≥6.0) all chicken breast treatments, but a larger proportion of consumers liked the 4 h LAPS and ES treatments when compared to the 0.75 h LAPS and ES treatments for all cooking methods. In addition, the consumers who indicated that baked chicken breast was highly acceptable preferred (P<0.05) 4 h LAPS over the 4 h ES samples. Based on sensory results, chicken breast meat from all stunning and deboning method combinations was highly acceptable to the majority of consumers, but the LAPS 4 h treatment had enhanced sensory characteristics when baked. © 2015 Poultry Science Association Inc.

Microstructure and Mechanical Properties of Friction Stir Welded Aluminum Alloy/Stainless Steel Lap Joints

NASA Astrophysics Data System (ADS)

Ogura, Tomo; Nishida, Taichi; Nishida, Hidehito; Yoshikawa, Syuhei; Yoshida, Takumi; Omichi, Noriko; Fujimoto, Mitsuo; Hirose, Akio

The mechanical properties and interfacial microstructure of an aluminum alloy/stainless steel dissimilar lap joint using friction stir welding (FSW) were characterized. In an FSWed A3003 aluminum alloy-SUS304 steel lap joint, the strength on the advancing side was larger than that at the retreating side. TEM observation indicated that a sound joint can be obtained from the stage of the formation of the amorphous layer owing to the mechanical alloying effects before the formation of intermetallic compounds. This lap joining technique was also successfully applied to A6061-T6 aluminum alloy-grooved SUS304 plates. The maximum tensile strength of the lap joint was approximately the same as that of the base alloy, however, the proof stress of the joint decreased with the dissolution of the β″ phase in the A6061 aluminium alloy, which is caused by the generation of heat during friction stir welding.

Better Axial Stiffness of a Bicortical Screw Construct Compared to a Cable Construct for Comminuted Vancouver B1 Proximal Femoral Fractures.

PubMed

Griffiths, Jamie T; Taheri, Arash; Day, Robert E; Yates, Piers J

2015-12-01

The aim of this study was to biomechanically evaluate the Locking attachment plate (LAP) construct in comparison to a Cable plate construct, for the fixation of periprosthetic femoral fractures after cemented total hip arthroplasty. Each construct incorporated a locking compression plate with bi-cortical locking screws for distal fixation. In the Cable construct, 2 cables and 2 uni-cortical locking screws were used for proximal fixation. In the LAP construct, the cables were replaced by a LAP with 4 bi-cortical locking screws. The LAP construct was significantly stiffer than the cable construct under axial load with a bone gap (P=0.01). The LAP construct offers better axial stiffness compared to the cable construct in the fixation of comminuted Vancouver B1 proximal femoral fractures. Crown Copyright © 2015. Published by Elsevier Inc. All rights reserved.

β-Lapachone attenuates mitochondrial dysfunction in MELAS cybrid cells.

PubMed

Jeong, Moon Hee; Kim, Jin Hwan; Seo, Kang-Sik; Kwak, Tae Hwan; Park, Woo Jin

2014-11-21

Mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) is a mitochondrial disease caused by mutations in the mitochondrial genome. This study investigated the efficacy of β-lapachone (β-lap), a natural quinone compound, in rescuing mitochondrial dysfunction in MELAS cybrid cells. β-Lap significantly restored energy production and mitochondrial membrane potential as well as normalized the elevated ROS level in MELAS cybrid cells. Additionally, β-lap reduced lactic acidosis and restored glucose uptake in the MELAS cybrid cells. Finally, β-lap activated Sirt1 by increasing the intracellular NAD(+)/NADH ratio, which was accompanied by increased mtDNA content. Two other quinone compounds (idebenone and CoQ10) that have rescued mitochondrial dysfunction in previous studies of MELAS cybrid cells had a minimal effect in the current study. Taken together, these results demonstrated that β-lap may provide a novel therapeutic modality for the treatment of MELAS. Copyright © 2014 Elsevier Inc. All rights reserved.

Learning Activity Packets for Milling Machines. Unit II--Horizontal Milling Machines.

ERIC Educational Resources Information Center

Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

This learning activity packet (LAP) outlines the study activities and performance tasks covered in a related curriculum guide on milling machines. The course of study in this LAP is intended to help students learn to set up and operate a horizontal mill. Tasks addressed in the LAP include mounting style "A" or "B" arbors and adjusting arbor…

Multivariate Analysis of High Through-Put Adhesively Bonded Single Lap Joints: Experimental and Workflow Protocols

DTIC Science & Technology

2016-06-01

unlimited. v List of Tables Table 1 Single-lap-joint experimental parameters ..............................................7 Table 2 Survey ...Joints: Experimental and Workflow Protocols by Robert E Jensen, Daniel C DeSchepper, and David P Flanagan Approved for...TR-7696 ● JUNE 2016 US Army Research Laboratory Multivariate Analysis of High Through-Put Adhesively Bonded Single Lap Joints: Experimental

Marketing and Distributive Education Curriculum Development Report. Final Report from December 31, 1981 to June 30, 1982.

ERIC Educational Resources Information Center

Florida Atlantic Univ., Boca Raton.

A project developed 10 learning activity packets (LAPs)--3 for Recreation/Tourism and 7 for Human Relations Skills. These LAPs were for use in high schools, vocational-technical centers, and community colleges and for teacher preservice and inservice staff development activities. The LAPs were produced by a team of Marketing and Distributive…

Global-Local Finite Element Analysis of Bonded Single-Lap Joints

NASA Technical Reports Server (NTRS)

Kilic, Bahattin; Madenci, Erdogan; Ambur, Damodar R.

2004-01-01

Adhesively bonded lap joints involve dissimilar material junctions and sharp changes in geometry, possibly leading to premature failure. Although the finite element method is well suited to model the bonded lap joints, traditional finite elements are incapable of correctly resolving the stress state at junctions of dissimilar materials because of the unbounded nature of the stresses. In order to facilitate the use of bonded lap joints in future structures, this study presents a finite element technique utilizing a global (special) element coupled with traditional elements. The global element includes the singular behavior at the junction of dissimilar materials with or without traction-free surfaces.

Left ventricular filling under elevated left atrial pressure

NASA Astrophysics Data System (ADS)

Gaddam, Manikantam; Samaee, Milad; Santhanakrishnan, Arvind

2017-11-01

Left atrial pressure (LAP) is elevated in diastolic dysfunction, where left ventricular (LV) filling is impaired due to increase in ventricular stiffness. The impact of increasing LAP and LV stiffness on intraventricular filling hemodynamics remains unclear. We conducted particle image velocimetry and hemodynamics measurements in a left heart simulator (LHS) under increasing LAP and LV stiffness at a heart rate of 70 bpm. The LHS consisted of a flexible-walled LV physical model fitted within a fluid-filled chamber. LV wall motion was generated by a piston pump that imparted pressure fluctuations in the chamber. Resistance and compliance elements in the flow loop were adjusted to obtain bulk physiological hemodynamics in the least stiff LV model. Two LV models of increasing stiffness were subsequently tested under unchanged loop settings. LAP was varied between 5-20 mm Hg for each LV model, by adjusting fluid level in a reservoir upstream of the LV. For constant LV stiffness, increasing LAP lowered cardiac output (CO), while ejection fraction (EF) and E/A ratio were increased. For constant LAP, increasing LV stiffness lowered CO and EF, and increased E/A ratio. The implications of these altered hemodynamics on intraventricular filling vortex characteristics will be presented.

Validation of a novel basic virtual reality simulator, the LAP-X, for training basic laparoscopic skills.

PubMed

Kawaguchi, Koji; Egi, Hiroyuki; Hattori, Minoru; Sawada, Hiroyuki; Suzuki, Takahisa; Ohdan, Hideki

2014-10-01

Virtual reality surgical simulators are becoming popular as a means of providing trainees with an opportunity to practice laparoscopic skills. The Lap-X (Epona Medical, Rotterdam, the Netherlands) is a novel VR simulator for training basic skills in laparoscopic surgery. The objective of this study was to validate the LAP-X laparoscopic virtual reality simulator by assessing the face and construct validity in order to determine whether the simulator is adequate for basic skills training. The face and content validity were evaluated using a structured questionnaire. To assess the construct validity, the participants, nine expert surgeons (median age: 40 (32-45)) (>100 laparoscopic procedures) and 11 novices performed three basic laparoscopic tasks using the Lap-X. The participants reported a high level of content validity. No significant differences were found between the expert surgeons and the novices (Ps > 0.246). The performance of the expert surgeons on the three tasks was significantly better than that of the novices in all parameters (Ps < 0.05). This study demonstrated the face, content and construct validity of the Lap-X. The Lap-X holds real potential as a home and hospital training device.

Impact of aggressive periodontitis and chronic periodontitis on oral health-related quality of life.

PubMed

Llanos, Alexandre Hugo; Silva, Carlos Guillermo Benítez; Ichimura, Karina Tamie; Rebeis, Estela Sanches; Giudicissi, Marcela; Romano, Marcelo Munhóes; Saraiva, Luciana

2018-01-01

The purpose of this cross-sectional study was to investigate the effect of different forms of periodontal diseases on Oral Health-Related Quality of Life (OHRQoL). Fifty-two patients with Aggressive Periodontitis (AP) or Chronic Periodontitis (CP) were included: nine patients with Localized Aggressive Periodontitis (LAP), thirty-three patients with Generalized Aggressive Periodontitis (GAP) and ten patients with Generalized Chronic Periodontitis (GCP). Oral Health Impact Profile questionnaires (OHIP-14) were distributed after a clinical examination that measured the following periodontal parameters: tooth loss, bleeding on probing (BoP), probing depth (PD), gingival recession (REC) and clinical attachment level (CAL). The global OHIP-14 score means were 10.6 for LAP, 16.5 for GAP, and 17.5 for GCP. A statistically significant difference (p < 0.01) was observed between the LAP group and the other two groups. There was significantly less bleeding and recession in the LAP group than in the patients with the generalized forms of periodontitis. LAP, GAP and GCP have an impact on patient quality of life when measured using the OHIP-14. Patients with GAP and GCP had poorer OHRQoL than LAP patients.

Instability analysis and free volume simulations of shear band directions and arrangements in notched metallic glasses

DOE PAGES

Li, Weidong; Gao, Yanfei; Bei, Hongbin

2016-10-10

As a commonly used method to enhance the ductility in bulk metallic glasses (BMGs), the introduction of geometric constraints blocks and confines the propagation of the shear bands, reduces the degree of plastic strain on each shear band so that the catastrophic failure is prevented or delayed, and promotes the formation of multiple shear bands. The clustering of multiple shear bands near notches is often interpreted as the reason for improved ductility. Experimental works on the shear band arrangements in notched metallic glasses have been extensively carried out, but a systematic theoretical study is lacking. Using instability theory that predictsmore » the onset of strain localization and the free-volume- based nite element simulations that predict the evolution of shear bands, this work reveals various categories of shear band arrangements in double edge notched BMGs with respect to the mode mixity of the applied stress fields. In conclusion, a mechanistic explanation is thus provided to a number of related experiments and especially the correlation between various types of shear bands and the stress state.« less

Intermittent bursts induced by double tearing mode reconnection

NASA Astrophysics Data System (ADS)

Wei, Lai; Wang, Zheng-Xiong

2014-06-01

Reversed magnetic shear (RMS) configuration is assumed to be the steady-state operation scenario for the future advanced tokamaks like International Thermonuclear Experimental Reactor. In this work, we numerically discover a phenomenon of violent intermittent bursts induced by self-organized double tearing mode (DTM) reconnection in the RMS configuration during the very long evolution, which may continuously lead to annular sawtooth crashes and thus badly impact the desired steady-state operation of the future advanced RMS tokamaks. The key process of the intermittent bursts in the off-axis region is similar to that of the typical sawtooth relaxation oscillation in the positive magnetic shear configuration. It is interestingly found that in the decay phase of the DTM reconnection, the zonal field significantly counteracts equilibrium field to make the magnetic shear between the two rational surfaces so weak that the residual self-generated vortices of the previous DTM burst are able to trigger a reverse DTM reconnection by curling the field lines.

Double-gap Alfvén eigenmodes: revisiting eigenmode interaction with the alfvén continuum.

PubMed

Gorelenkov, N N

2005-12-31

A new type of global shear Alfvén eigenmode is found in tokamak plasmas where the mode localization is in the region intersecting the Alfvén continuum. The eigenmode is formed by the coupling of two solutions from two adjacent gaps (akin to potential wells) in the shear Alfvén continuum. For tokamak plasmas with reversed magnetic shear, it is shown that the toroidicity-induced solution tunnels through the continuum to match the ellipticity-induced Alfvén eigenmode so that the resulting solution is continuous at the point of resonance with the continuum. The existence of these double-gap Alfvén eigenmodes allows for potentially new ways of coupling edge fields to the plasma core in conditions where the core region is conventionally considered inaccessible. Implications include new approaches to heating and current drive in fusion plasmas as well as its possible use as a core diagnostic in burning plasmas.

Preparation and Testing of Plant Seed Meal-based Wood Adhesives

PubMed Central

He, Zhongqi; Chapital, Dorselyn C.

2015-01-01

Recently, the interest in plant seed meal-based products as wood adhesives has steadily increased, as these plant raw materials are considered renewable and environment-friendly. These natural products may serve as alternatives to petroleum-based adhesives to ease environmental and sustainability concerns. This work demonstrates the preparation and testing of the plant seed-based wood adhesives using cottonseed and soy meal as raw materials. In addition to untreated meals, water washed meals and protein isolates are prepared and tested. Adhesive slurries are prepared by mixing a freeze-dried meal product with deionized water (3:25 w/w) for 2 hr. Each adhesive preparation is applied to one end of 2 wood veneer strips using a brush. The tacky adhesive coated areas of the wood veneer strips are lapped and glued by hot-pressing. Adhesive strength is reported as the shear strength of the bonded wood specimen at break. Water resistance of the adhesives is measured by the change in shear strength of the bonded wood specimens at break after water soaking. This protocol allows one to assess plant seed-based agricultural products as suitable candidates for substitution of synthetic-based wood adhesives. Adjustments to the adhesive formulation with or without additives and bonding conditions could optimize their adhesive properties for various practical applications. PMID:25867092

Preparation and testing of plant seed meal-based wood adhesives.

PubMed

He, Zhongqi; Chapital, Dorselyn C

2015-03-05

Recently, the interest in plant seed meal-based products as wood adhesives has steadily increased, as these plant raw materials are considered renewable and environment-friendly. These natural products may serve as alternatives to petroleum-based adhesives to ease environmental and sustainability concerns. This work demonstrates the preparation and testing of the plant seed-based wood adhesives using cottonseed and soy meal as raw materials. In addition to untreated meals, water washed meals and protein isolates are prepared and tested. Adhesive slurries are prepared by mixing a freeze-dried meal product with deionized water (3:25 w/w) for 2 hr. Each adhesive preparation is applied to one end of 2 wood veneer strips using a brush. The tacky adhesive coated areas of the wood veneer strips are lapped and glued by hot-pressing. Adhesive strength is reported as the shear strength of the bonded wood specimen at break. Water resistance of the adhesives is measured by the change in shear strength of the bonded wood specimens at break after water soaking. This protocol allows one to assess plant seed-based agricultural products as suitable candidates for substitution of synthetic-based wood adhesives. Adjustments to the adhesive formulation with or without additives and bonding conditions could optimize their adhesive properties for various practical applications.

Correlation analysis of the variation of weld seam and tensile strength in laser welding of galvanized steel

NASA Astrophysics Data System (ADS)

Sinha, Amit Kumar; Kim, Duck Young; Ceglarek, Darek

2013-10-01

Many advantages of laser welding technology such as high speed and non-contact welding make the use of the technology more attractive in the automotive industry. Many studies have been conducted to search the optimal welding condition experimentally that ensure the joining quality of laser welding that relies both on welding system configuration and welding parameter specification. Both non-destructive and destructive techniques, for example, ultrasonic inspection and tensile test are widely used in practice for estimating the joining quality. Non-destructive techniques are attractive as a rapid quality testing method despite relatively low accuracy. In this paper, we examine the relationship between the variation of weld seam and tensile shear strength in the laser welding of galvanized steel in a lap joint configuration in order to investigate the potential of the variation of weld seam as a joining quality estimator. From the experimental analysis, we identify a trend in between maximum tensile shear strength and the variation of weld seam that clearly supports the fact that laser welded parts having larger variation in the weld seam usually have lower tensile strength. The discovered relationship leads us to conclude that the variation of weld seam can be used as an indirect non-destructive testing method for estimating the tensile strength of the welded parts.

An Experimental Study on Micro Clinching of Metal Foils with Cutting by Laser Shock Forming

PubMed Central

Wang, Xiao; Li, Cong; Ma, Youjuan; Shen, Zongbao; Sun, Xianqing; Sha, Chaofei; Gao, Shuai; Li, Liyin; Liu, Huixia

2016-01-01

This paper describes a novel technique for joining similar and dissimilar metal foils, namely micro clinching with cutting by laser shock forming. A series of experiments were conducted to study the deformation behavior of single layer material, during which many important process parameters were determined. The process window of the 1060 pure aluminum foils and annealed copper foils produced by micro clinching with cutting was analyzed. Moreover, similar material combination (annealed copper foils) and dissimilar material combination (1060 pure aluminum foils and 304 stainless steel foils) were successfully achieved. The effect of laser energy on the interlock and minimum thickness of upper foils was investigated. In addition, the mechanical strength of different material combinations joined by micro clinching with cutting was measured in single lap shearing tests. According to the achieved results, this novel technique is more suitable for material combinations where the upper foil is thicker than lower foil. With the increase of laser energy, the interlock increased while the minimum thickness of upper foil decreased gradually. The shear strength of 1060 pure aluminum foils and 304 stainless steel foils combination was three times as large as that of 1060 pure aluminum foils and annealed copper foils combination. PMID:28773692

On the Process-Related Rivet Microstructural Evolution, Material Flow and Mechanical Properties of Ti-6Al-4V/GFRP Friction-Riveted Joints.

PubMed

Borba, Natascha Z; Afonso, Conrado R M; Blaga, Lucian; Dos Santos, Jorge F; Canto, Leonardo B; Amancio-Filho, Sergio T

2017-02-15

In the current work, process-related thermo-mechanical changes in the rivet microstructure, joint local and global mechanical properties, and their correlation with the rivet plastic deformation regime were investigated for Ti-6Al-4V (rivet) and glass-fiber-reinforced polyester (GF-P) friction-riveted joints of a single polymeric base plate. Joints displaying similar quasi-static mechanical performance to conventional bolted joints were selected for detailed characterization. The mechanical performance was assessed on lap shear specimens, whereby the friction-riveted joints were connected with AA2198 gussets. Two levels of energy input were used, resulting in process temperatures varying from 460 ± 130 °C to 758 ± 56 °C and fast cooling rates (178 ± 15 °C/s, 59 ± 15 °C/s). A complex final microstructure was identified in the rivet. Whereas equiaxial α-grains with β-phase precipitated in their grain boundaries were identified in the rivet heat-affected zone, refined α' martensite, Widmanstätten structures and β-fleck domains were present in the plastically deformed rivet volume. The transition from equiaxed to acicular structures resulted in an increase of up to 24% in microhardness in comparison to the base material. A study on the rivet material flow through microtexture of the α-Ti phase and β-fleck orientation revealed a strong effect of shear stress and forging which induced simple shear deformation. By combining advanced microstructural analysis techniques with local mechanical testing and temperature measurement, the nature of the complex rivet plastic deformational regime could be determined.

On Temperature Rise Within the Shear Bands in Bulk Metallic Glasses

NASA Astrophysics Data System (ADS)

Bazlov, A. I.; Churyumov, A. Yu.; Buchet, M.; Louzguine-Luzgin, D. V.

2018-05-01

Room temperature deformation process in a bulk metallic glassy sample was studied by using a hydraulic thermomechanical simulator. The temperature rise during each separate shear band propagation event was measured with a high data acquisition frequency by a thermocouple welded to the sample. Calculation showed that when propagation of the well developed shear bands takes place along the entire sample the temperature inside the shear band should be close to the glass-transition temperature. It was also possible to resolve the temporal stress distribution and a double-stage character of stress drops was also observed. The obtained results are compared with the literature data obtained by infrared camera measurements and the results of finite elements modeling.

On Temperature Rise Within the Shear Bands in Bulk Metallic Glasses

NASA Astrophysics Data System (ADS)

Bazlov, A. I.; Churyumov, A. Yu.; Buchet, M.; Louzguine-Luzgin, D. V.

2018-03-01

Room temperature deformation process in a bulk metallic glassy sample was studied by using a hydraulic thermomechanical simulator. The temperature rise during each separate shear band propagation event was measured with a high data acquisition frequency by a thermocouple welded to the sample. Calculation showed that when propagation of the well developed shear bands takes place along the entire sample the temperature inside the shear band should be close to the glass-transition temperature. It was also possible to resolve the temporal stress distribution and a double-stage character of stress drops was also observed. The obtained results are compared with the literature data obtained by infrared camera measurements and the results of finite elements modeling.

Shading aboveground L-joint and lap-joint tests : comparison of white pine and sugar maple test assemblies

Treesearch

Carol A. Clausen; Daniel L. Lindner

2011-01-01

Five-year performance ratings are presented for two types of untreated, uncoated wood joints (L and lap) in aboveground tests under shaded conditions. The effect of shading on moisture entrapment in pine and maple L and lap joints was evaluated in a moderate decay zone (Madison, Wisconsin). Variations were observed between wood species, visual ratings, joint type,...

75 FR 35356 - Airworthiness Directives; The Boeing Company Model 747-100, 747-200B, and 747-200F Series Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-22

...-related skin cracks and corrosion of the skin panel lap joints in the fuselage upper lobe, and repair if... inspections of the fuselage skin at the upper lobe skin lap joints for cracks and evidence of corrosion, and... correct fatigue cracking and corrosion in the fuselage upper lobe skin lap joints, which could lead to...

Comparative effects of amlodipine and benazepril on left atrial pressure in dogs with experimentally-induced mitral valve regurgitation.

PubMed

Suzuki, Shuji; Fukushima, Ryuji; Ishikawa, Taisuke; Yamamoto, Yuta; Hamabe, Lina; Kim, Soomin; Yoshiyuki, Rieko; Machida, Noboru; Tanaka, Ryou

2012-09-18

One of the purposes of treatment for dogs with mitral regurgitation (MR) is lowering left atrial pressure (LAP). There has been few study of the amlodipine in dogs with MR and amlodipine's effect on LAP has not been fully evaluated in a quantitative manner because of difficulties in directly measuring LAP. The objective of our study was to compare the short-term effects of amlodipine (0.2 mg/kg PO q12h) vs benazepril (0.5 mg/kg PO q12h), on LAP and echocardiographic parameters in five beagle dogs with experimentally-induced MR. LAP of eight dogs that has own control were measured using radiotelemetry system at baseline and again on days 1, 2, 3, 4, 5, 6, 7 of the drug administration. Mean LAP decreased significantly after amlodipine (11.20 ± 4.19 mmHg vs 14.61 ± 3.81 mmHg at baseline, p < .01) but not after benazepril treatment (13.19 ± 3.47 mmHg, p > .05). LAP was lower after 7 days of amlodipine treatment than after 7 days of benazepril treatment. Significant reduction was seen for the first time 4 days after the administration amlodipine. The rate of the maximal area of the regurgitant jet signals to the left atrium area (ARJ/LAA) of the amlodipine treatment was significantly lower (p < .05) after 7 days compared to baseline. Other echocardiographic parameters did not change significantly. LAP was significantly decreased after amlodipine treatment in dogs with surgically-induced MR but not after benazepril treatment. Although this study did not focus on adverse effects, amlodipine may be an effective drug for helping the patients with acute onset of severe MR, such as rupture of chordae tendinae or end stage patients were the LAP is likely to be elevated. Additional studies in clinical patients with degenerative mitral valve disease and acute chordal rupture are warranted because the blood-pressure lowering effects of amlodipine can decrease renal perfusion and this can further activate the RAAS.

Comparative effects of amlodipine and benazepril on Left Atrial Pressure in Dogs with experimentally-induced Mitral Valve Regurgitation

PubMed Central

2012-01-01

Background One of the purposes of treatment for dogs with mitral regurgitation (MR) is lowering left atrial pressure (LAP). There has been few study of the amlodipine in dogs with MR and amlodipine’s effect on LAP has not been fully evaluated in a quantitative manner because of difficulties in directly measuring LAP. The objective of our study was to compare the short-term effects of amlodipine (0.2 mg/kg PO q12h) vs benazepril (0.5 mg/kg PO q12h), on LAP and echocardiographic parameters in five beagle dogs with experimentally-induced MR. LAP of eight dogs that has own control were measured using radiotelemetry system at baseline and again on days 1, 2, 3, 4, 5, 6, 7 of the drug administration. Results Mean LAP decreased significantly after amlodipine (11.20 ± 4.19 mmHg vs 14.61 ± 3.81 mmHg at baseline, p < .01) but not after benazepril treatment (13.19 ± 3.47 mmHg, p > .05). LAP was lower after 7 days of amlodipine treatment than after 7 days of benazepril treatment. Significant reduction was seen for the first time 4 days after the administration amlodipine. The rate of the maximal area of the regurgitant jet signals to the left atrium area (ARJ/LAA) of the amlodipine treatment was significantly lower (p < .05) after 7 days compared to baseline. Other echocardiographic parameters did not change significantly. Conclusions LAP was significantly decreased after amlodipine treatment in dogs with surgically-induced MR but not after benazepril treatment. Although this study did not focus on adverse effects, amlodipine may be an effective drug for helping the patients with acute onset of severe MR, such as rupture of chordae tendinae or end stage patients were the LAP is likely to be elevated. Additional studies in clinical patients with degenerative mitral valve disease and acute chordal rupture are warranted because the blood-pressure lowering effects of amlodipine can decrease renal perfusion and this can further activate the RAAS. PMID:22989022

Stresses in adhesively bonded joints: A closed form solution. [plate theory

NASA Technical Reports Server (NTRS)

Delale, F.; Erdogan, F.; Aydinoglu, M. N.

1980-01-01

The plane strain of adhesively bonded structures which consist of two different orthotropic adherents is considered. Assuming that the thicknesses of the adherends are constant and are small in relation to the lateral dimensions of the bonded region, the adherends are treated as plates. The transverse shear effects in the adherends and the in-plane normal strain in the adhesive are taken into account. The problem is reduced to a system of differential equations for the adhesive stresses which is solved in closed form. A single lap joint and a stiffened plate under various loading conditions are considered as examples. To verify the basic trend of the solutions obtained from the plate theory a sample problem is solved by using the finite element method and by treating the adherends and the adhesive as elastic continua. The plate theory not only predicts the correct trend for the adhesive stresses but also gives rather surprisingly accurate results.

Materials research for High Speed Civil Transport and generic hypersonics: Adhesive durability

NASA Technical Reports Server (NTRS)

Allen, Mark R.

1995-01-01

This report covers a portion of an ongoing investigation of the durability of adhesives for the High Speed Civil Transport (HSCT) program. Candidate HSCT adhesives need to possess the high-temperature capability required for supersonic flight. This program was designed to initiate an understanding of the behavior of candidate HSCT materials when subjected to combined mechanical and thermal loads. Two adhesives (K3A and FM57) and two adherends (IM7/K3B polymeric composite and the titanium alloy Ti-6Al-4V) were used to fabricate thick adherend lap shear specimens. Due to processing problems, only the FM57/titanium bonds could be fabricated successfully. These are currently undergoing thermomechanical fatigue (TMF) testing. There is an acute need for an adhesive to secondarily bond polymeric composite adherends or, alternately, polymeric composites that remain stable at the processing temperatures of today's adhesives.

Laminar flow control perforated wing panel development

NASA Technical Reports Server (NTRS)

Fischler, J. E.

1986-01-01

Many structural concepts for a wing leading edge laminar flow control hybrid panel were analytically investigated. After many small, medium, and large tests, the selected design was verified. New analytic methods were developed to combine porous titanium sheet bonded to a substructure of fiberglass and carbon/epoxy cloth. At -65 and +160 F test conditions, the critical bond of the porous titanium to the composite failed at lower than anticipated test loads. New cure cycles, design improvements, and test improvements significantly improved the strength and reduced the deflections from thermal and lateral loadings. The wave tolerance limits for turbulence were not exceeded. Consideration of the beam column midbay deflections from the combinations of the axial and lateral loadings and thermal bowing at -65 F, room temperature, and +160 F were included. Many lap shear tests were performed at several cure cycles. Results indicate that sufficient verification was obtained to fabricate a demonstration vehicle.

Autonomous sensing of composites with carbon nanotubes for structural health monitoring

NASA Astrophysics Data System (ADS)

Liu, Yingtao; Yekani Fard, Masoud; Rajadas, Abhishek; Chattopadhyay, Aditi

2012-04-01

The development of structural health monitoring techniques leads to the integration of sensing capability within engineering structures. This study investigates the application of multi walled carbon nanotubes in polymer matrix composites for autonomous damage detection through changes in electrical resistance. The autonomous sensing capabilities of fiber reinforced nanocomposites are studied under multiple loading conditions including tension loads. Single-lap joints with different joint lengths are tested. Acoustic emission sensing is used to validate the matrix crack propagation. A digital image correlation system is used to measure the shear strain field of the joint area. The joints with 1.5 inch length have better autonomous sensing capabilities than those with 0.5 inch length. The autonomous sensing capabilities of nanocomposites are found to be sensitive to crack propagation and can revolutionize the research on composite structural health management in the near future.

Design of Friction Stir Spot Welding Tools by Using a Novel Thermal-Mechanical Approach

PubMed Central

Su, Zheng-Ming; Qiu, Qi-Hong; Lin, Pai-Chen

2016-01-01

A simple thermal-mechanical model for friction stir spot welding (FSSW) was developed to obtain similar weld performance for different weld tools. Use of the thermal-mechanical model and a combined approach enabled the design of weld tools for various sizes but similar qualities. Three weld tools for weld radii of 4, 5, and 6 mm were made to join 6061-T6 aluminum sheets. Performance evaluations of the three weld tools compared fracture behavior, microstructure, micro-hardness distribution, and welding temperature of welds in lap-shear specimens. For welds made by the three weld tools under identical processing conditions, failure loads were approximately proportional to tool size. Failure modes, microstructures, and micro-hardness distributions were similar. Welding temperatures correlated with frictional heat generation rate densities. Because the three weld tools sufficiently met all design objectives, the proposed approach is considered a simple and feasible guideline for preliminary tool design. PMID:28773800

Design of Friction Stir Spot Welding Tools by Using a Novel Thermal-Mechanical Approach.

PubMed

Su, Zheng-Ming; Qiu, Qi-Hong; Lin, Pai-Chen

2016-08-09

A simple thermal-mechanical model for friction stir spot welding (FSSW) was developed to obtain similar weld performance for different weld tools. Use of the thermal-mechanical model and a combined approach enabled the design of weld tools for various sizes but similar qualities. Three weld tools for weld radii of 4, 5, and 6 mm were made to join 6061-T6 aluminum sheets. Performance evaluations of the three weld tools compared fracture behavior, microstructure, micro-hardness distribution, and welding temperature of welds in lap-shear specimens. For welds made by the three weld tools under identical processing conditions, failure loads were approximately proportional to tool size. Failure modes, microstructures, and micro-hardness distributions were similar. Welding temperatures correlated with frictional heat generation rate densities. Because the three weld tools sufficiently met all design objectives, the proposed approach is considered a simple and feasible guideline for preliminary tool design.

Solid-state Bonding of Superplastic Aluminum Alloy 7475 Sheet

NASA Technical Reports Server (NTRS)

Byun, T. D. S.; Vastava, R. B.

1985-01-01

Experimental works were carried out to study the feasibility of solid state bonding of superplastic aluminum 7475 sheet. Amount of deformation, bonding time, surface cleaning method and intermediate layer were the process parameters investigated. Other parameters, held constant by the superplastic forming condition which is required to obtain a concurrent solid state bonding, are bonding temperature, bonding pressure and atmosphere. Bond integrity was evaluated through metallographic examination, X-ray line scan analysis, SEM fractographic analysis and lap shear tests. The early results of the development program indicated that sound solid state bonding was accomplished for this high strength 7475 alloy with significant amounts of deformation. A thin intermediate layer of the soft 5052 aluminum alloy aided in achieving a solid state bonding by reducing the required amount of plastic deformation at the interface. Bond strength was substantially increased by a post bond heat treatment.

Effect of Steel Galvanization on the Microstructure and Mechanical Performances of Planar Magnetic Pulse Welds of Aluminum and Steel

NASA Astrophysics Data System (ADS)

Avettand-Fènoël, M.-N.; Khalil, C.; Taillard, R.; Racineux, G.

2018-07-01

For the first time, planar joints between pure aluminum and galvanized or uncoated DP450 steel joints have been developed via magnetic pulse welding. Both present a wavy interface. The microstructure of the interfacial zone differs according to the joint. With uncoated steel, the interface is composed of discrete 2.5- µm-thick FeAl3 intermetallic compounds and Fe penetration lamellae, whereas the interface of the pure Al-galvanized steel joint is bilayered and composed of a 10-nm-thick (Al)Zn solid solution and a few micrometers thick aggregate of Al- and Zn-based grains, arranged from the Al side to the Zn coating. Even if the nature of the interfacial zone differs with or without the steel coating, both welds present rather similar maximum tensile forces and ductility in shear lap testing.

Microstructures and Mechanical Properties of Friction Stir Spot Welded Aluminum Alloy AA2014

NASA Astrophysics Data System (ADS)

Babu, S.; Sankar, V. S.; Janaki Ram, G. D.; Venkitakrishnan, P. V.; Madhusudhan Reddy, G.; Prasad Rao, K.

2013-01-01

Friction stir spot welding (FSSW) is a relatively recent development, which can provide a superior alternative to resistance spot welding and riveting for fabrication of aluminum sheet metal structures. In the current work, FSSW experiments were conducted in 3-mm thick sheets of aluminum alloy 2014 in T4 and T6 conditions, with and without Alclad layers. The effects of tool geometry and welding process parameters on joint formation were investigated. A good correlation between process parameters, bond width, hook height, joint strength, and fracture mode was observed. The presence of Alclad layers and the base metal temper condition were found to have no major effect on joint formation and joint strength. Friction stir spot welds produced under optimum conditions were found to be superior to riveted joints in lap-shear and cross-tension tests. The prospects of FSSW in aluminum sheet metal fabrication are discussed.

NR-150B2 adhesive development

NASA Technical Reports Server (NTRS)

Blatz, P. S.

1978-01-01

Adhesive based polyimide solutions which are more easily processed than conventional aromatic polyimide systems and show potential for use for extended times at 589K are discussed. The adhesive system is based on a solution containing diglyme as the solvent and 2,2 bis(3',4'-dicarboxyphenyl)hexafluoropropane, paraphenylenediamine, and oxydianiline. The replacement of N-methylpyrrolidone with diglyme as the solvent was found to improve the adhesive strengths of lap shear samples and simplify the processing conditions for bonding both titanium and graphite fiber/polyimide matrix resin composites. Information was obtained on the effects of various environments including high humidity, immersion in jet fuel and methylethylketone on aluminum filled adhesive bonds. The adhesive was also evaluated in wide area bonds and flatwise tensile specimens using titanium honeycomb and composite face sheets. It was indicated that the developed adhesive system has the potential for use in applications requiring long term exposure to at least 589K (600 F).

Modified Phenylethynyl Containing Imides for Secondary Bonding: Non-Autoclave, Low Temperature Processable Adhesives

NASA Technical Reports Server (NTRS)

Dezern, James F. (Technical Monitor); Chang, Alice C.

1999-01-01

As part of a program to develop structural adhesives for high performance aerospace applications, research continued on the development of modified phenylethynyl containing imides, LaRC(trademark)MPEIs. In previous reports, the polymer properties were controlled by varying the molecular weight, the amount of branching, and the phenylethynyl content and by blending with low molecular weight materials. This research involves changing the flexibility in the copolyimide backbone of the branched, phenylethynyl terminated adhesives. These adhesives exhibit excellent processability at pressures as low as 15 psi and temperatures as low as 288 C. The Ti/Ti lap shear specimens are processable in an autoclave or a temperature programmable oven under a vacuum bag at 288-300 C without external pressure. The cured polymers exhibit high mechanical properties and excellent solvent resistance. The chemistry and properties of these adhesives are presented.

Effect of Zinc Coatings on Joint Properties and Interfacial Reactions in Aluminum to Steel Ultrasonic Spot Welding

NASA Astrophysics Data System (ADS)

Haddadi, F.; Strong, D.; Prangnell, P. B.

2012-03-01

Dissimilar joining of aluminum to steel sheet in multimaterial automotive structures is an important potential application of ultrasonic spot welding (USW). Here, the weldability of different zinc-coated steels with aluminum is discussed, using a 2.5-kW USW welder. Results show that soft hot-dipped zinc (DX56-Z)-coated steel results in better weld performance than hard (galv-annealed) zinc coatings (DX53-ZF). For Al to hard galv-annealed-coated steel welds, lap shear strengths reached a maximum of ~80% of the strength of an Al-Al joint after a 1.0 s welding time. In comparison, welds between Al6111-T4 and hot dipped soft zinc-coated steel took longer to achieve the same maximum strength, but nearly matched the Al-Al joint properties. The reasons for these different behaviors are discussed in terms of the interfacial reactions between the weld members.

Advanced thermoplastic resins, phase 2

NASA Technical Reports Server (NTRS)

Brown, A. M.; Hill, S. G.; Falcone, A.

1991-01-01

High temperature structural resins are required for use on advanced aerospace vehicles as adhesives and composite matrices. NASA-Langley developed polyimide resins were evaluated as high temperature structural adhesives for metal to metal bonding and as composite matrices. Adhesive tapes were prepared on glass scrim fabric from solutions of polyamide acids of the semicrystalline polyimide LARC-CPI, developed at the NASA-Langley Research Center. Using 6Al-4V titanium adherends, high lap shear bond strengths were obtained at ambient temperature (45.2 MPa, 6550 psi) and acceptable strengths were obtained at elevated temperature (14.0 MPa, 2030 psi) using the Pasa-Jell 107 conversion coating on the titanium and a bonding pressure of 1.38 MPa (200 psi). Average zero degree composite tensile and compressive strengths of 1290 MPa (187 ksi) and 883 MPa (128 ksi) respectively were obtained at ambient temperature with unsized AS-4 carbon fiber reinforcement.

Environmental Aging of Scotch-Weld(TradeMark) AF-555M Structural Adhesive in Composite to Composite Bonds

NASA Technical Reports Server (NTRS)

Hou, Tan-Hung; Miner, Gilda A.; Lowther, Sharon E.; Connell, John W.; Baughman, James M.

2010-01-01

Fiber reinforced resin matrix composites have found increased usage in recent years. Due to the lack of service history of these relatively new material systems, their long-term aging performance is not well established. In this study, adhesive bonds were prepared by the secondary bonding of Scotch-Weld(TradeMark) AF-555M between pre-cured adherends comprised of T800H/3900-2 uni-directional laminate. The adherends were co-cured with wet peel-ply for surface preparation. Each bond-line of single-lap-shear (SLS) specimen was measured to determine thickness and inspected visually for voids. A three-year environmental aging plan for the SLS specimens at 82 C and 85% relative humidity was initiated. SLS strengths were measured for both controls and aged specimens at room temperature and 82 C. The aging results of strength retention and failure modes to date are reported.

Effect of Steel Galvanization on the Microstructure and Mechanical Performances of Planar Magnetic Pulse Welds of Aluminum and Steel

NASA Astrophysics Data System (ADS)

Avettand-Fènoël, M.-N.; Khalil, C.; Taillard, R.; Racineux, G.

2018-05-01

For the first time, planar joints between pure aluminum and galvanized or uncoated DP450 steel joints have been developed via magnetic pulse welding. Both present a wavy interface. The microstructure of the interfacial zone differs according to the joint. With uncoated steel, the interface is composed of discrete 2.5-µm-thick FeAl3 intermetallic compounds and Fe penetration lamellae, whereas the interface of the pure Al-galvanized steel joint is bilayered and composed of a 10-nm-thick (Al)Zn solid solution and a few micrometers thick aggregate of Al- and Zn-based grains, arranged from the Al side to the Zn coating. Even if the nature of the interfacial zone differs with or without the steel coating, both welds present rather similar maximum tensile forces and ductility in shear lap testing.

Debonding characteristics of adhesively bonded woven Kevlar composites

NASA Technical Reports Server (NTRS)

Mall, S.; Johnson, W. S.

1988-01-01

The fatigue damage mechanism of an adhesively bonded joint between fabric reinforced composite adherends was investigated with cracked-lap-shear specimens. Two bonded systems were studied: fabric Kevlar 49/5208 epoxy adherends bonded together with either EC 3445 or FM-300 adhesive. For each bonded system, two specimen geometries were tested. In all specimens tested, fatigue damage occurred in the form of cyclic debonding; however, the woven Kevlar specimens gave significantly slower debond growth rates and higher fracture toughness than previously found in the nonwoven adherend specimens. The surfaces for the woven adherends were not smooth; rather, they had regular crests (high spots) and troughs (low spots) due to the weave pattern. Radiographs of the specimens and examination of their failure surfaces revealed that fiber bridging occurred between the crests of the two adherends in the debonded region. The observed improvements in debond growth resistance and static fracture toughness are attributed to this bridging.

The double well mass filter

DOE PAGES

Gueroult, Renaud; Rax, Jean -Marcel; Fisch, Nathaniel J.

2014-02-03

Various mass filter concepts based on rotating plasmas have been suggested with the specific purpose of nuclear waste remediation. We report on a new rotating mass filter combining radial separation with axial extraction. Lastly, the radial separation of the masses is the result of a “double-well” in effective radial potential in rotating plasma with a sheared rotation profile.

The Impact of Laparoscopic Approaches on Short-term Outcomes in Patients Undergoing Liver Surgery for Metastatic Tumors.

PubMed

Karagkounis, Georgios; Seicean, Andreea; Berber, Eren

2015-06-01

To compare the perioperative outcomes associated with open and laparoscopic (LAP) surgical approaches for liver metastases. The American College of Surgeons National Surgical Quality Improvement Program database was used to identify all adult patients who underwent surgical therapy for metastatic liver tumors between 2006 and 2012 (N=7684). Patients who underwent >1 procedure were excluded. Logistic regression after matching on propensity scores was used to assess the association between surgical approaches and perioperative outcomes. A total of 4555 patients underwent open resection, 387 LAP resection, 297 open radiofrequency ablation (RFA), and 265 LAP RFA. In propensity-matched samples (over 95% of patients successfully matched), there was no significant difference between LAP resection and LAP RFA in perioperative complications and length of stay and both compared favorably with their open counterparts. Minimally invasive approaches for secondary hepatic malignancies were associated with improved postoperative morbidity and length of stay and should be preferred in appropriate patients.

Determination of L-AP4-bound human mGlu8 receptor amino terminal domain structure and the molecular basis for L-AP4’s group III mGlu receptor functional potency and selectivity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schkeryantz, Jeffery M.; Chen, Qi; Ho, Joseph D.

Here, L-2-Amino-4-phosphonobutyric acid (L-AP4) is a known potent and selective agonist for the Group III mGlu receptors. However, it does not show any selectivity among the individual group III mGlu subtypes. In order to understand the molecular basis for this group selectivity, we solved the first human mGlu8 amino terminal domain (ATD) crystal structures in complex with L-glu and L-AP4. In comparison with other published L-glu-bound mGlu ATD structures, we have observed L-glu binds in a significantly different manner in mGlu1. Furthermore, these new structures provided evidence that both the electronic and steric nature of the distal phosphate of L-AP4more » contribute to its exquisite Group III functional agonist potency and selectivity.« less

LC3-associated phagocytosis initiated by integrin ITGAM-ITGB2/Mac-1 enhances immunity to Listeria monocytogenes.

PubMed

Herb, Marc; Gluschko, Alexander; Schramm, Michael

2018-06-20

The macroautophagic/autophagic machinery cannot only target cell-endogenous components but also intracellular pathogenic bacteria such as Listeria monocytogenes. Listeria are targeted both by canonical autophagy and by a noncanonical form of autophagy referred to as LC3-associated phagocytosis (LAP). The molecular mechanisms involved and whether these processes contribute to anti-listerial immunity or rather provide Listeria with a replicative niche for persistent infection, however, remained unknown. Recently, using an in vivo mouse infection model, we have been able to demonstrate that Listeria in tissue macrophages are targeted exclusively by LAP. Furthermore, our data show that LAP is required for killing of Listeria by macrophages and thereby contributes to anti-listerial immunity of mice, whereas canonical autophagy is completely dispensable. Moreover, we have elucidated the molecular mechanisms that trigger LAP of Listeria and identified the integrin ITGAM-ITGB2/Mac-1/CR3/integrin α M ß 2 as the receptor that initiates LAP in response to Listeria infection.

Effect of Viscosity on Fuel Leakage Between Lapped Plungers and Sleeves and on the Discharge from a Pump-Injection System

NASA Technical Reports Server (NTRS)

Rothrock, A M; Marsh, E T

1935-01-01

Test data and analysis show that the rate of fuel leakage between a lapped plunger and sleeve varies directly with the density of the fuel, the diameter of the plunger, the pressure producing the leakage, and the cube of the mean clearance between the plunger and sleeve. The rate varies inversely as the length of the lapped fit and the viscosity of the fuel. With a mean clearance between the plunger and sleeve of 0.0001 inch the leakage amounts to approximately 0.2 percent of the fuel injected with gasoline and as low as 0.01 percent with diesel fuel oils. With this mean clearance an effective seal is obtained when the length of the lap is three times the diameter of the lap. The deformation of the sleeve and plunger under pressure is sufficient to change the rate of leakage appreciably from that which would be obtained if the clearance was constant under pressure.

Aspergillus Cell Wall Melanin Blocks LC3-Associated Phagocytosis to Promote Pathogenicity.

PubMed

Akoumianaki, Tonia; Kyrmizi, Irene; Valsecchi, Isabel; Gresnigt, Mark S; Samonis, George; Drakos, Elias; Boumpas, Dimitrios; Muszkieta, Laetitia; Prevost, Marie-Christine; Kontoyiannis, Dimitrios P; Chavakis, Triantafyllos; Netea, Mihai G; van de Veerdonk, Frank L; Brakhage, Axel A; El-Benna, Jamel; Beauvais, Anne; Latge, Jean-Paul; Chamilos, Georgios

2016-01-13

Concealing pathogen-associated molecular patterns (PAMPs) is a principal strategy used by fungi to avoid immune recognition. Surface exposure of PAMPs during germination can leave the pathogen vulnerable. Accordingly, β-glucan surface exposure during Aspergillus fumigatus germination activates an Atg5-dependent autophagy pathway termed LC3-associated phagocytosis (LAP), which promotes fungal killing. We found that LAP activation also requires the genetic, biochemical or biological (germination) removal of A. fumigatus cell wall melanin. The attenuated virulence of melanin-deficient A. fumigatus is restored in Atg5-deficient macrophages and in mice upon conditional inactivation of Atg5 in hematopoietic cells. Mechanistically, Aspergillus melanin inhibits NADPH oxidase-dependent activation of LAP by excluding the p22phox subunit from the phagosome. Thus, two events that occur concomitantly during germination of airborne fungi, surface exposure of PAMPs and melanin removal, are necessary for LAP activation and fungal killing. LAP blockade is a general property of melanin pigments, a finding with broad physiological implications. Copyright © 2016 Elsevier Inc. All rights reserved.

Behavior of Fiber Glass Bolts, Rock Bolts and Cable Bolts in Shear

NASA Astrophysics Data System (ADS)

Li, Xuwei; Aziz, Naj; Mirzaghorbanali, Ali; Nemcik, Jan

2016-07-01

This paper experimentally compares the shear behavior of fiber glass (FG) bolt, rock bolt (steel rebar bolt) and cable bolt for the bolt contribution to bolted concrete surface shear strength, and bolt failure mode. Two double shear apparatuses of different size were used for the study. The tensile strength, the shear strength and the deformation modulus of bolt control the shear behavior of a sheared bolted joint. Since the strength and deformation modulus of FG bolt, rock bolt and cable bolt obtained from uniaxial tensile tests are different, their shear behavior in reinforcing joints is accordingly different. Test results showed that the shear stiffness of FG bolted joints decreased gradually from the beginning to end, while the shear stiffness of joints reinforced by rock bolt and cable bolt decreased bi-linearly, which is clearly consistent with their tensile deformation modulus. The bolted joint shear stiffness was highly influenced by bolt pretension in the high stiffness stage for both rock bolt and cable bolt, but not in the low stiffness stage. The rock bolt contribution to joint shear strength standardised by the bolt tensile strength was the largest, followed by cable bolts, then FG bolts. Both the rock bolts and cable bolts tended to fail in tension, while FG bolts in shear due to their low shear strength and constant deformation modulus.

Owning and Operating a Small Business. A Guide for Industrial Cooperative Training Programs. Learning Activity Package No. 14.

ERIC Educational Resources Information Center

Braden, Robert P.; Remzi, Kerim

This learning activity package (LAP) on starting a small business is designed for student self-study. It will help students learn about their aptitudes and abilities as well as about the basics of running a small business. Following a list of learning objectives, the LAP contains a pretest (answer key provided at the end of the LAP). Ten learning…

Adhesives: Test Method, Group Assignment, and Categorization Guide for High-Loading-Rate Applications Preparation and Testing of Single Lap Joints (Ver. 2.2, Unlimited)

DTIC Science & Technology

2016-04-01

Gerard Chaney, and Charles Pergantis Weapons and Materials Research Directorate, ARL Coatings, Corrosion, and Engineered Polymers Branch (CCEPB...SUBJECT TERMS single lap joint, adhesive, sample preparation, testing, database, metadata, material pedigree, ISO 16. SECURITY CLASSIFICATION OF: 17...temperature/water immersion conditioning test for lap-joint test specimens using the test tubes and convection oven method

A servo-controlled canine model of stable severe ischemic left ventricular failure.

PubMed

Wagner, Richard L; Hood, William B; Howland, Peter A

2009-12-01

Reversible left ventricular failure was produced in conscious dogs by compromise of the coronary circulation. In animals with prior left anterior descending coronary artery occlusion, mean left atrial pressure (LAP) was incorporated into an automatic feedback control system used to inflate a balloon cuff on the circumflex (Cfx) coronary artery. The system could produce stable increases in LAP to 15-20 mm Hg. The dominating system transfer function was the ratio of LAP to balloon volume (BV), which was characterized by a fixed delay (5 s), with LAP/BV = (8e(-jomegatau ))/(0.02 + jomega). The system was stabilized by a phase lead network to reduce oscillations of LAP. A total of seven experiments were conducted in three dogs, and testing of inotropic agents was possible in three experiments under stable conditions with the pump off after an hour or more of operation. Problems encountered were 0.003-0.008 Hz oscillations in LAP in three experiments, which could usually be controlled by reducing the system gain. Late stage ventricular fibrillation occurred in all three animals, but defibrillation was easily accomplished after deflating the Cfx balloon. This system produces reversible left ventricular failure solely due to ischemia, thus closely simulating clinical heart failure due to coronary insufficiency.

Long-Term Clinical Response to Treatment and Maintenance of Localized Aggressive Periodontitis: A Cohort Study

PubMed Central

Miller, Karina A. F. S.; Branco-de-Almeida, Luciana S.; Wolf, Sandra; Hovencamp, Nicole; Treloar, Tina; Harrison, Peter; Aukhil, Ikramuddin; Gong, Yan; Shaddox, Luciana M.

2016-01-01

Aim To evaluate long-term clinical response to periodontal therapy and maintenance in localized aggressive periodontitis (LAP). Materials and Methods One hundred forty-one African-Americans diagnosed with LAP, aged 5–25 years, were enrolled. Patients underwent periodontal mechanical debridement plus one week of amoxicillin/metronidazole. Mechanical therapy was repeated as needed and clinical parameters were recorded at baseline, 3, 6, 12, 18, and 24 months, and 2 additional annual follow-up visits after treatment. Radiographs from primary dentition of patients with LAP in permanent dentition, and additional healthy siblings (HS) were analyzed retrospectively. Results Periodontal therapy significantly improved probing depth and clinical attachment level up to 4 years (mean reductions: 2.18 ± 1.03 and 2.80 ± 1.43 mm, respectively). Percentage of affected sites was reduced at all timepoints and maintained up to 4 years. Noncompliance with antibiotics/appointments negatively affected the treatment response. Ninety percent of LAP patients in permanent dentition and 32% of HS presented radiographic bone loss in primary dentition. Conclusions Mechanical debridement with one-week of systemic antibiotics along with proper periodontal maintenance was effective in the treatment and successful maintenance of LAP for up to 4 years. LAP in permanent dentition may be preceded in the primary dentition. Clinicaltrials.gov #NCT01330719. PMID:27767222

High shear enrichment improves the performance of the anodophilic microbial consortium in a microbial fuel cell

PubMed Central

Pham, Hai The; Boon, Nico; Aelterman, Peter; Clauwaert, Peter; De Schamphelaire, Liesje; Van Oostveldt, Patrick; Verbeken, Kim; Rabaey, Korneel; Verstraete, Willy

2008-01-01

Summary In many microbial bioreactors, high shear rates result in strong attachment of microbes and dense biofilms. In this study, high shear rates were applied to enrich an anodophilic microbial consortium in a microbial fuel cell (MFC). Enrichment at a shear rate of about 120 s−1 resulted in the production of a current and power output two to three times higher than those in the case of low shear rates (around 0.3 s−1). Biomass and biofilm analyses showed that the anodic biofilm from the MFC enriched under high shear rate conditions, in comparison with that under low shear rate conditions, had a doubled average thickness and the biomass density increased with a factor 5. The microbial community of the former, as analysed by DGGE, was significantly different from that of the latter. The results showed that enrichment by applying high shear rates in an MFC can result in a specific electrochemically active biofilm that is thicker and denser and attaches better, and hence has a better performance. PMID:21261869

Effect of double-layer application on bond quality of adhesive systems.

PubMed

Fujiwara, Satoshi; Takamizawa, Toshiki; Barkmeier, Wayne W; Tsujimoto, Akimasa; Imai, Arisa; Watanabe, Hidehiko; Erickson, Robert L; Latta, Mark A; Nakatsuka, Toshiyuki; Miyazaki, Masashi

2018-01-01

The aim of this study was to determine the effect of double-layer application of universal adhesives on the bond quality and compare to other adhesive systems. Two universal adhesives used were in this study: Scotchbond Universal (SU), [3M ESPE] and Prime & Bond elect (PE), [Dentsply Caulk]. The conventional single-step self-etch adhesives G-ӕnial Bond (GB), [GC Corporation.] and BeautiBond (BB), [Shofu Inc.], and a two-step self-etch adhesive, Optibond XTR (OX), [Kerr Corporation], were used as comparison adhesives. Shear bond strengths (SBS) and shear fatigue strengths (SFS) to human enamel and dentin were measured in single application mode and double application mode. For each test condition, 15 specimens were prepared for SBS testing and 30 specimens for SFS testing. Enamel and dentin SBS of the universal adhesives in the double application mode were significantly higher than those of the single application mode. In addition, the universal adhesives in the double application mode had significantly higher dentin SFS values than those of the single application mode. The two-step self-etch adhesive OX tended to have lower bond strengths in the double application mode, regardless of the test method or adherent substrate. The double application mode is effective in enhancing SBS and SFS of universal adhesives, but not conventional two-step self-etch adhesives. These results suggest that, although the double application mode may enhance the bonding quality of a universal adhesive, it may be counter-productive for two-step self-etch adhesives in clinical use. Copyright © 2017 Elsevier Ltd. All rights reserved.

Dual functional extracellular recording using a light-addressable potentiometric sensor for bitter signal transduction.

PubMed

Du, Liping; Wang, Jian; Chen, Wei; Zhao, Luhang; Wu, Chunsheng; Wang, Ping

2018-08-31

This paper presents a dual functional extracellular recording biosensor based on a light-addressable potentiometric sensor (LAPS). The design and fabrication of this biosensor make it possible to record both extracellular membrane potential changes and ATP release from a single taste bud cell for the first time. For detecting ATP release, LAPS chip was functionalized with ATP-sensitive DNA aptamer by covalent immobilization. Taste bud cells isolated from rat were cultured on LAPS surface. When the desired single taste bud cell was illuminated by modulated light, ATP release from single taste bud cells can be measured by recording the shifts of bias voltage-photocurrent curves (I-V curves) when the LAPS chip is working in discrete mode. On the other hand, extracellular membrane potential changes can be monitored by recording the fluctuation of LAPS photocurrent when the LAPS chip is working in continuous mode. The results show this biosensor can effectively record the enhancive effect of the bitter substance and inhibitory effect of the carbenoxolone (CBX) on the extracellular membrane potential changes and ATP release of single taste bud cells. In addition, the inhibitory effect of CBX also confirms LAPS extracellular recordings are originated from bitter signal transduction. It is proved this biosensor is suitable for extracellular recording of ATP release and membrane potential changes of single taste bud cells. It is suggested this biosensor could be applied to investigating taste signal transduction at the single-cell level as well as applied to other types of cells which have similar functions to taste bud cells. Copyright © 2018 Elsevier B.V. All rights reserved.

Impaired circulating CD4+ LAP+ regulatory T cells in patients with acute coronary syndrome and its mechanistic study.

PubMed

Zhu, Zheng-Feng; Meng, Kai; Zhong, Yu-Cheng; Qi, Liang; Mao, Xiao-Bo; Yu, Kun-Wu; Zhang, Wei; Zhu, Peng-Fei; Ren, Ze-Peng; Wu, Bang-Wei; Ji, Qin-Wei; Wang, Xiang; Zeng, Qiu-Tang

2014-01-01

CD4(+) latency-associated peptide (LAP)(+) regulatory T cells (Tregs) are a newly discovered T cell subset in humans and the role of these cells in patients with acute coronary syndrome (ACS) has not been explored. We designed to investigate whether circulating frequency and function of CD4(+)LAP(+) Tregs are defective in ACS. One hundred eleven ACS patients (acute myocardial infarction and unstable angina) and 117 control patients were enrolled in the study. The control patients consisted of chronic stable angina (CSA) and chest pain syndrome (CPS). The frequencies of circulating CD4(+)LAP(+) Tregs and the expression of the transmembrane protein glycoprotein-A repetitions predominant (GARP) on CD4(+) T cells were determined by flow cytometry. The function of CD4(+)LAP(+) Tregs was detected using thymidine uptake. Serum interleukin-10 (IL-10) and transforming growth factor-β protein (TGF-β) levels were detected using ELISA and expression of GARP mRNA in peripheral blood mononuclear cells (PBMCs) was measured by real time-polymerase chain reaction. We found ACS patients had a significantly lower frequency of circulating CD4(+)LAP(+) Tregs, and the function of these cells was reduced compared to controls. The expression of GARP in CD4(+) T cells and the serum levels of TGF-β in ACS patients were lower than those of control patients. The serum levels of IL-10 were similar between the two cohorts. A novel regulatory T cell subset, defined as CD4(+)LAP(+) T cells is defective in ACS patients.

Patterns of low acuity patient presentations to emergency departments in New South Wales, Australia.

PubMed

Stephens, Alexandre S; Broome, Richard A

2017-06-01

To explore the patterns of low acuity patient (LAP) presentations to EDs in New South Wales (NSW), Australia. Retrospective study of NSW public hospital ED presentations between January 2013 and December 2014 that were registered in the NSW Emergency Department Data Collection (n = 409 035). LAPs were defined according to the Australian Institute of Health and Welfare (AIHW), Sprivulis and multiple ACEM methods. Multivariable logistic regression was used to assess the adjusted odds of LAP ED presentation by a suite of sociodemographic factors. The percentage of LAPs varied considerably by definition, being as high as 54.7% (inner regional areas) and as low as 3.2% (major cities) using revised ACEM methods modified to contain unlimited consultation times or consultation times of 15 min or less, respectively. For each method, higher proportions of LAPs were observed in inner regional and remote/very remote areas relative to major cities. LAP ED presentations, based on ACEM definition with 1 h or 15 min consultation times, were greater in younger patients, increased during out of business hours and weekends, and decreased with increasing general practitioner (GP) density. The percentage of LAPs varied substantially by definition, and further work is required to validate the methods, particularly around the appropriateness of length of consultation time with ACEM, between different hospitals and remoteness areas. Age was strongly associated with low acuity, with substantial effects also observed for GP density, and attendances during out of hours and weekends. © 2017 Australasian College for Emergency Medicine and Australasian Society for Emergency Medicine.

Economic Impact of Laparoscopic Conversion to Open in Left Colon Resections.

PubMed

Etter, Katherine; Davis, Brad; Roy, Sanjoy; Kalsekar, Iftekhar; Yoo, Andrew

2017-01-01

Studies have shown economic and clinical advantages of laparoscopic left-colon resections. Laparoscopic conversion to open is an important surgical outcome. We estimated conversion incidence, identified risk factors, and measured the clinical and economic impact. In this retrospective study, we used the Premier Perspective database to analyze left-sided colectomies from 2009 to 2014. Operating room time (ORT), length of stay (LOS), total hospital cost (2014 U.S. dollars); along with incidence of in-hospital clinical outcomes (anastomotic leak surrogate [Leak], transfusion, and mortality) were evaluated. Multivariable models accounting for hospital clustering were used to identify conversion risk factors and analyze the effect of conversion on economic and clinical outcomes. A total of 41,417 patients: 8,468 left hemicolectomy and 32,949 sigmoidectomy were identified. Lap-Conversion incidence was 13.3% (95% CI, 12.9-13.7). Adjusted mean LOS (±SE) days was significantly lower for the Lap-Successful group (4.9 compared with Lap-Conversion 6.8 and Open-Planned 7.0), but Lap-Conversion and Open-Planned had similar LOS. Adjusted mean cost was higher for Lap-Conversion $20,165 compared to Open-Planned $18,797; but this difference was smaller than the cost savings for Lap-Successful $16,206 ± $219. Open-Planned had lower odds of Leak compared to Lap-Conversion. Open-Planned and Lap-Conversion had similar odds of transfusion and mortality. Conversion risk factors included inflammatory bowel disease and left-hemicolectomy. Colorectal specialists were associated with 38% decreased odds of conversion. Successful laparoscopic surgery was the most cost effective, with decreased LOS and odds of blood transfusion, leak surrogate, and mortality. Conversion was the most expensive and had increased odds of leak surrogate, but similar LOS compared to Open-Planned. The beneficial effect size of successful laparoscopic surgery was larger than the negative effect of conversion compared to Open-Planned.

A randomized comparison of laparoscopic, magnetically anchored, and flexible endoscopic cameras in performance and workload between laparoscopic and single-incision surgery.

PubMed

Arain, Nabeel A; Cadeddu, Jeffrey A; Best, Sara L; Roshek, Thomas; Chang, Victoria; Hogg, Deborah C; Bergs, Richard; Fernandez, Raul; Webb, Erin M; Scott, Daniel J

2012-04-01

This study aimed to evaluate the surgeon performance and workload of a next-generation magnetically anchored camera compared with laparoscopic and flexible endoscopic imaging systems for laparoscopic and single-site laparoscopy (SSL) settings. The cameras included a 5-mm 30° laparoscope (LAP), a magnetically anchored (MAGS) camera, and a flexible endoscope (ENDO). The three camera systems were evaluated using standardized optical characteristic tests. Each system was used in random order for visualization during performance of a standardized suturing task by four surgeons. Each participant performed three to five consecutive repetitions as a surgeon and also served as a camera driver for other surgeons. Ex vivo testing was conducted in a laparoscopic multiport and SSL layout using a box trainer. In vivo testing was performed only in the multiport configuration and used a previously validated live porcine Nissen model. Optical testing showed superior resolution for MAGS at 5 and 10 cm compared with LAP or ENDO. The field of view ranged from 39 to 99°. The depth of focus was almost three times greater for MAGS (6-270 mm) than for LAP (2-88 mm) or ENDO (1-93 mm). Both ex vivo and in vivo multiport combined surgeon performance was significantly better for LAP than for ENDO, but no significant differences were detected for MAGS. For multiport testing, workload ratings were significantly less ex vivo for LAP and MAGS than for ENDO and less in vivo for LAP than for MAGS or ENDO. For ex vivo SSL, no significant performance differences were detected, but camera drivers rated the workload significantly less for MAGS than for LAP or ENDO. The data suggest that the improved imaging element of the next-generation MAGS camera has optical and performance characteristics that meet or exceed those of the LAP or ENDO systems and that the MAGS camera may be especially useful for SSL. Further refinements of the MAGS camera are encouraged.

Economic Impact of Laparoscopic Conversion to Open in Left Colon Resections

PubMed Central

Etter, Katherine; Davis, Brad; Roy, Sanjoy; Kalsekar, Iftekhar

2017-01-01

Background and Objectives: Studies have shown economic and clinical advantages of laparoscopic left-colon resections. Laparoscopic conversion to open is an important surgical outcome. We estimated conversion incidence, identified risk factors, and measured the clinical and economic impact. Methods: In this retrospective study, we used the Premier Perspective database to analyze left-sided colectomies from 2009 to 2014. Operating room time (ORT), length of stay (LOS), total hospital cost (2014 U.S. dollars); along with incidence of in-hospital clinical outcomes (anastomotic leak surrogate [Leak], transfusion, and mortality) were evaluated. Multivariable models accounting for hospital clustering were used to identify conversion risk factors and analyze the effect of conversion on economic and clinical outcomes. Results: A total of 41,417 patients: 8,468 left hemicolectomy and 32,949 sigmoidectomy were identified. Lap-Conversion incidence was 13.3% (95% CI, 12.9–13.7). Adjusted mean LOS (±SE) days was significantly lower for the Lap-Successful group (4.9 compared with Lap-Conversion 6.8 and Open-Planned 7.0), but Lap-Conversion and Open-Planned had similar LOS. Adjusted mean cost was higher for Lap-Conversion $20,165 compared to Open-Planned $18,797; but this difference was smaller than the cost savings for Lap-Successful $16,206 ± $219. Open-Planned had lower odds of Leak compared to Lap-Conversion. Open-Planned and Lap-Conversion had similar odds of transfusion and mortality. Conversion risk factors included inflammatory bowel disease and left-hemicolectomy. Colorectal specialists were associated with 38% decreased odds of conversion. Conclusions: Successful laparoscopic surgery was the most cost effective, with decreased LOS and odds of blood transfusion, leak surrogate, and mortality. Conversion was the most expensive and had increased odds of leak surrogate, but similar LOS compared to Open-Planned. The beneficial effect size of successful laparoscopic surgery was larger than the negative effect of conversion compared to Open-Planned. PMID:28890650

Radiative effects of light-absorbing particles deposited in snow over Himalayas using WRF-Chem simulations

NASA Astrophysics Data System (ADS)

Sarangi, C.; Qian, Y.; Painter, T. H.; Liu, Y.; Lin, G.; Wang, H.

2017-12-01

Radiative forcing induced by light-absorbing particles (LAP) deposited on snow is an important surface forcing. It has been debated that an aerosol-induced increase in atmospheric and surface warming over Tibetan Plateau (TP) prior to the South Asian summer monsoon can have a significant effect on the regional thermodynamics and South Asian monsoon circulation. However, knowledge about the radiative effects due to deposition of LAP in snow over TP is limited. In this study we have used a high-resolution WRF-Chem (coupled with online chemistry and snow-LAP-radiation model) simulations during 2013-2014 to estimate the spatio-temporal variation in LAP deposition on snow, specifically black carbon (BC) and dust particles, in Himalayas. Simulated distributions in meteorology, aerosol concentrations, snow albedo, snow grain size and snow depth are evaluated against satellite and in-situ measurements. The spatio-temporal change in snow albedo and snow grain size with variation in LAP deposition is investigated and the resulting shortwave LAP radiative forcing at surface is calculated. The LAP-radiative forcing due to aerosol deposition, both BC and dust, is higher in magnitude over Himalayan slopes (terrain height below 4 km) compared to that over TP (terrain height above 4 km). We found that the shortwave aerosol radiative forcing efficiency at surface due to increase in deposited mass of BC particles in snow layer ( 25 (W/m2)/ (mg/m2)) is manifold higher than the efficiency of dust particles ( 0.1 (W/m2)/ (mg/m2)) over TP. However, the radiative forcing of dust deposited in snow is similar in magnitude (maximum 20-30 W/m2) to that of BC deposited in snow over TP. This is mainly because the amount of dust deposited in snow over TP can be about 100 times greater than the amount of BC deposited in snow during polluted conditions. The impact of LAP on surface energy balance, snow melting and atmospheric thermodynamics is also examined.

Positive correlation between motion analysis data on the LapMentor virtual reality laparoscopic surgical simulator and the results from videotape assessment of real laparoscopic surgeries.

PubMed

Matsuda, Tadashi; McDougall, Elspeth M; Ono, Yoshinari; Hattori, Ryohei; Baba, Shiro; Iwamura, Masatsugu; Terachi, Toshiro; Naito, Seiji; Clayman, Ralph V

2012-11-01

We studied the construct validity of the LapMentor, a virtual reality laparoscopic surgical simulator, and the correlation between the data collected on the LapMentor and the results of video assessment of real laparoscopic surgeries. Ninety-two urologists were tested on basic skill tasks No. 3 (SK3) to No. 8 (SK8) on the LapMentor. They were divided into three groups: Group A (n=25) had no experience with laparoscopic surgeries as a chief surgeon; group B (n=33) had <35 experiences; and group C (n=34) had ≥35 experiences. Group scores on the accuracy, efficacy, and time of the tasks were compared. Forty physicians with ≥20 experiences supplied unedited videotapes showing a laparoscopic nephrectomy or an adrenalectomy in its entirety, and the videos were assessed in a blinded fashion by expert referees. Correlations between the videotape score (VS) and the performances on the LapMentor were analyzed. Group C showed significantly better outcomes than group A in the accuracy (SK5) (P=0.013), efficacy (SK8) (P=0.014), or speed (SKs 3 and 8) (P=0.009 and P=0.002, respectively) of the performances of LapMentor. Group B showed significantly better outcomes than group A in the speed and efficacy of the performances in SK8 (P=0.011 and P=0.029, respectively). Analyses of motion analysis data of LapMentor demonstrated that smooth and ideal movement of instruments is more important than speed of the movement of instruments to achieve accurate performances in each task. Multiple linear regression analysis indicated that the average score of the accuracy in SK4, 5, and 8 had significant positive correlation with VS (P=0.01). This study demonstrated the construct and predictive validity of the LapMentor basic skill tasks, supporting their possible usefulness for the preclinical evaluation of laparoscopic skills.

A non-orthogonal material model of woven composites in the preforming process

DOE PAGES

Zhang, Weizhao; Ren, Huaqing; Liang, Biao; ...

2017-05-04

Woven composites are considered as a promising material choice for lightweight applications. An improved non-orthogonal material model that can decouple the strong tension and weak shear behaviour of the woven composite under large shear deformation is proposed for simulating the preforming of woven composites. The tension, shear and compression moduli in the model are calibrated using the tension, bias-extension and bending experiments, respectively. The interaction between the composite layers is characterized by a sliding test. The newly developed material model is implemented in the commercial finite element software LS-DYNA® and validated by a double dome study.

KC-135 aero-optical turbulent boundary layer/shear layer experiment revisited

NASA Technical Reports Server (NTRS)

Craig, J.; Allen, C.

1987-01-01

The aero-optical effects associated with propagating a laser beam through both an aircraft turbulent boundary layer and artificially generated shear layers are examined. The data present comparisons from observed optical performance with those inferred from aerodynamic measurements of unsteady density and correlation lengths within the same random flow fields. Using optical instrumentation with tens of microsecond temporal resolution through a finite aperture, optical performance degradation was determined and contrasted with the infinite aperture time averaged aerodynamic measurement. In addition, the optical data were artificially clipped to compare to theoretical scaling calculations. Optical instrumentation consisted of a custom Q switched Nd:Yag double pulsed laser, and a holographic camera which recorded the random flow field in a double pass, double pulse mode. Aerodynamic parameters were measured using hot film anemometer probes and a five hole pressure probe. Each technique is described with its associated theoretical basis for comparison. The effects of finite aperture and spatial and temporal frequencies of the random flow are considered.

Intermonolayer Friction and Surface Shear Viscosity of Lipid Bilayer Membranes

PubMed Central

den Otter, W. K.; Shkulipa, S. A.

2007-01-01

The flow behavior of lipid bilayer membranes is characterized by a surface viscosity for in-plane shear deformations, and an intermonolayer friction coefficient for slip between the two leaflets of the bilayer. Both properties have been studied for a variety of coarse-grained double-tailed model lipids, using equilibrium and nonequilibrium molecular dynamics simulations. For lipids with two identical tails, the surface shear viscosity rises rapidly with tail length, while the intermonolayer friction coefficient is less sensitive to the tail length. Interdigitation of lipid tails across the bilayer midsurface, as observed for lipids with two distinct tails, strongly enhances the intermonolayer friction coefficient, but hardly affects the surface shear viscosity. The simulation results are compared against the available experimental data. PMID:17468168

Effect of Eccentricity in Compound Droplets Subject to a Simple Shear Flow

NASA Astrophysics Data System (ADS)

Kim, Sangkyu; Dabiri, Sadegh

2016-11-01

A double emulsion, or a compound droplet, is a system where two liquids are separated by an immiscible third liquid, thereby forming an emulsion inside an emulsion. Compound drops benefit from this separation in applications such food sciences, microfluidics, pharmaceutical engineering, and polymer sciences. While the subjects of double emulsion preparations, deformations, and breakup mechanisms are well-explored, the time-evolution of non-concentric compound drops has received far less analytical or computational scrutiny. In this work, we present computational results using finite volume method with front-tracking approach for initially spherical and non-concentric compound drops in a shear flow. Our findings for low Reynolds number flows show that: 1. The surrounding shear flow to the outer drop induces a rotational velocity field inside it, causing the inner drop to tumble with the flow, 2. the tumbling motion persists in time, and acts to increase the eccentricity of the compound drop, and 3. the hemisection-plane to the outer drop that is aligned with the plane of the simple shear defines an unstable equilibrium for inner drop's center, and the inner drop continuously drifts away from that plane. This work suggests a means of favorably configuring compound drops suitable for breakups, and helps to understand their migration in channel flows.

Screening Adhesively Bonded Single-Lap-Joint Testing Results Using Nonlinear Calculation Parameters

DTIC Science & Technology

2012-03-01

versus displacement response for single-lap-joints bonded with damage-tolerant adhe- sives, such the polyurea adhesive plotted in Figure 2, is much...displacement response for a single-lap-joint bonded with a polyurea adhesive. Complex x-y plots are commonly fitted using the Levenberg-Marquardt...expected decrease in maximum strength for the polyurea in compar- ison to the epoxy, which could have been obtained using a traditional analysis approach

Large-scale Advanced Prop-fan (LAP) technology assessment report

NASA Technical Reports Server (NTRS)

Degeorge, C. L.

1988-01-01

The technologically significant findings and accomplishments of the Large Scale Advanced Prop-Fan (LAP) program in the areas of aerodynamics, aeroelasticity, acoustics and materials and fabrication are described. The extent to which the program goals related to these disciplines were achieved is discussed, and recommendations for additional research are presented. The LAP program consisted of the design, manufacture and testing of a near full-scale Prop-Fan or advanced turboprop capable of operating efficiently at speeds to Mach .8. An aeroelastically scaled model of the LAP was also designed and fabricated. The goal of the program was to acquire data on Prop-Fan performance that would indicate the technology readiness of Prop-Fans for practical applications in commercial and military aviation.

Age of Lunar Meteorite LAP02205 and Implications for Impact-Sampling of Planetary Surfaces

NASA Technical Reports Server (NTRS)

Nyquist, L. E.; Shih, C.-Y.; Reese, Y.; Bogard, D. D.

2005-01-01

We have measured the age of lunar meteorite LAP02205 by the Rb-Sr and Ar-Ar methods. Sm-Nd analyses are in progress. The Rb-Sr and Ar-Ar ages indicate a crystallization age of approx. 3 Ga. Comparing the ages of LAP02205 and other lunar mare basaltic meteorites to mare surface ages based on the density of impact craters shows no significant bias in impact- sampling of lunar mare surfaces. Comparing the isotopic and geochemical data for LAP02205 to those for other lunar mare basalts suggests that it is a younger variant of the type of volcanism that produced the Apollo 12 basalts. Representative impact-sampling of the lunar surface

Rectangle-capped and tilted micropillar array for enhanced anisotropic anti-shearing in biomimetic adhesion

PubMed Central

Wang, Yue; Li, Xiangming; Tian, Hongmiao; Hu, Hong; Tian, Yu; Shao, Jinyou; Ding, Yucheng

2015-01-01

Dry adhesion observed in the feet of various small creatures has attracted considerable attention owing to the unique advantages such as self-cleaning, adaptability to rough surfaces along with repeatable and reversible adhesiveness. Among these advantages, for practical applications, proper detachability is critical for dry adhesives with artificial microstructures. In this study, we present a microstructured array consisting of both asymmetric rectangle-capped tip and tilted shafts, which produce an orthogonal anisotropy of the shearing strength along the long and short dimensions of the tip, with a maximum anti-shearing in the two directions along the longer dimension. Meanwhile, the tilt feature can enhance anisotropic shearing adhesion by increasing shearing strength in the forward shearing direction and decreasing strength in the reverse shearing direction along the short dimension of the tip, leading to a minimum anti-shearing in only one of the two directions along the shorter dimension of the rectangular tip. Such a microstructured adhesive with only one weak shearing direction, leading to well-controlled attachment and detachment of the adhesive, is created in our experiment by conventional double-sided exposure of a photoresist followed by a moulding process. PMID:25808338

Corrosion of steel members strengthenened with carbon fiber reinforced polymer sheets

NASA Astrophysics Data System (ADS)

Bumadian, Ibrahim

Due to many years of service at several cases of exposure at various environments there are many of steel bridges which are in need of rehabilitation. The infrastructure needs upgrading, repair or maintenance, and also strengthening, but by using an alternative as retrofits methods. The alternative retrofit method, which used fiber reinforced polymer (FRP) composite materials which their strength materials comes largely from the fiber such as carbon, glass, and aramid fiber. Of the most important materials used in the rehabilitation of infrastructure is a composite material newly developed in bonded externally carbon fiber and polymer (CFRP) sheets, which has achieved remarkable success in the rehabilitation and upgrading of structural members. This technique has many disadvantages one of them is galvanic corrosion. This study presents the effect of galvanic corrosion on the interfacial strength between carbon fiber reinforced polymer (CFRP) sheets and a steel substrate. A total of 35 double-lap joint specimens and 19 beams specimens are prepared and exposed to an aggressive service environment in conjunction with an electrical potential method accelerating corrosion damage. Six test categories are planned at a typical exposure interval of 12 hours, including five specimens per category for double-lap joint specimens. And six test categories are planned at a typical exposure interval of 12 hours, including three specimens per category for Beam section specimens. In addition one beam section specimen is control. The degree of corrosion is measured. Fourier transform infrared (FTIR) reflectance spectroscopy has been used to monitor and confirm the proposed corrosion mechanisms on the surface of CFRP. In this study we are using FTIR-spectroscopic measurement systems in the mid infrared (MIR) wavelength region (4000 - 400) cm-1 to monitor characteristic spectral features. Upon completion of corrosion processes, all specimens are monotonically loaded until failure occurs to measure their residual capacity. A relationship between the level of galvanic corrosion and the failure characteristics of steel-composite interface is established.

Cardiopulmonary function and oxygen delivery during total liquid ventilation.

PubMed

Tsagogiorgas, Charalambos; Alb, Markus; Herrmann, Peter; Quintel, Michael; Meinhardt, Juergen P

2011-10-01

Total liquid ventilation (TLV) with perfluorocarbons has shown to improve cardiopulmonary function in the injured and immature lung; however there remains controversy over the normal lung. Hemodynamic effects of TLV in the normal lung currently remain undetermined. This study compared changes in cardiopulmonary and circulatory function caused by either liquid or gas tidal volume ventilation. In a prospective, controlled study, 12 non-injured anesthetized, adult New Zealand rabbits were primarily conventionally gas-ventilated (CGV). After instrumentation for continuous recording of arterial (AP), central venous (CVP), left artrial (LAP), pulmonary arterial pressures (PAP), and cardiac output (CO) animals were randomized into (1) CGV group and (2) TLV group. In the TLV group partial liquid ventilation was initiated with instillation of perfluoroctylbromide (12 ml/kg). After 15 min, TLV was established for 3 hr applying a volume-controlled, pressure-limited, time-cycled ventilation mode using a double-piston configured TLV. Controls (CGV) remained gas-ventilated throughout the experiment. During TLV, heart rate, CO, PAP, MAP, CVP, and LAP as well as derived hemodynamic variables, arterial and mixed venous blood gases, oxygen delivery, PVR, and SVR did not differ significantly compared to CGV. Liquid tidal volumes suitable for long-term TLV in non-injured rabbits do not significantly impair CO, blood pressure, and oxygen dynamics when compared to CGV. Copyright © 2011 Wiley-Liss, Inc.

Experimental Investigation on High-Cycle Fatigue of Inconel 625 Superalloy Brazed Joints

NASA Astrophysics Data System (ADS)

Chen, Jianqiang; Demers, Vincent; Turner, Daniel P.; Bocher, Philippe

2018-04-01

The high-cycle fatigue performance and crack growth pattern of transient liquid phase-brazed joints in a nickel-based superalloy Inconel 625 were studied. Assemblies with different geometries and types of overlaps were vacuum-brazed using the brazing paste Palnicro-36M in conditions such as to generate eutectic-free joints. This optimal microstructure provides the brazed assemblies with static mechanical strength corresponding to that of the base metal. However, eutectic micro-constituents were observed in the fillet region of the brazed assembly due to an incomplete isothermal solidification within this large volume of filler metal. The fatigue performance increased significantly with the overlap distance for single-lap joints, and the best performance was found for double-lap joints. It was demonstrated that these apparent changes in fatigue properties according to the specimen geometry can be rationalized when looking at the fatigue data as a function of the local stress state at the fillet radii. Fatigue cracks were nucleated from brittle eutectic phases located at the surface of the fillet region. Their propagation occurred through the bimodal microstructure of fillet and the diffusion region to reach the base metal. High levels of crack path tortuosity were observed, suggesting that the ductile phases found in the microstructure may act as a potential crack stopper. The fillet region must be considered as the critical region of a brazed assembly for fatigue applications.

Socio-Economic and Health Status of Leprosy Affected Person: A Study in Jharkhand.

PubMed

Majumder, N

2015-01-01

The study has been conducted in the Potka Block of East Singhbhum district of the state of Jharkhand. The district is mainly dominated by indigenous tribes, such as, Santhal, Munda, Ho, Bhumiz, Kharia, and Sabar. The unit of analysis of the study was an individual. The objectives were to: a) Understand the socio-economic and health status of LAP, b) Know the health seeking behavior and problems faced by the LAP, c) Assess the utilization of the programs related to Leprosy eradication in the study area and d), Suggest various measures for improving the socio-economic and health status of LAP. Fifty Leprosy affected persons (LAP) from the Potka block; comprising of 20% of LAP of that area have been selected as the study sample by using the method of Multi-Stage Random Sampling, with equal representation of men and women. The LAPs included leprosy patients, leprosy treated people and their family members. 39/50 (78%) of the respondents are illiterates and only 3/11 (6%) among the literate population have crossed matriculation and above. This seems to have resulted in the respondent's low level of awareness about the disease, resulting in delayed treatment. 14/25 (56%) percent of female and 13/25 (52%) of male respondents are considered untouchable by their natal families, thus forced to stay in congested leprosy colonies resulting in other social and health related issues. It was observed that leprosy cured children,and also children of LAP are being denied admission iany school, due to the social stigma attached to it. 27/50 (54%)of leprosy patients and leprosy cured people (mostly with visible deformities) were found to practice begging as their sole means of livelihood. Many LAPs are also engaged in cultivation and small scale business particularly among the rural population. An amount of gender disparity was also observed in the employment pattern among the LAPs. Among the, respondents 15/25 (60%) of the females are beggars as compared to 12/25 (48%) of the male respondents, 5/25 (25%) of males are each engaged in cultivation and small scale businesses in comparison to 1/25 (4%) of female and 6/25 (24%) of the female respondents are unemployed as compared to 2/25 (8%) of male respondents. It was observed that only 30% of the respondents were satisfied with the government treatment, 26% partially satisfied and rest were not satisfied with the government leprosy care system. Most of them wanted to seek treatment from the private health care providers. Overall this study reflects the poor socio-economic conditions of the LAPs. Though results of this exploratory study cannot be extrapolated to country or region or state without studying the situation in detail, it highlights the need for more in-depth studies and of government intervention in the form of encouraging awareness activities in the communities, engaging NGOs im case detection and after care service provision and rehabilitation of the LAPs.

L-Cysteine and L-AP4 microinjections in the rat caudal ventrolateral medulla decrease arterial blood pressure.

PubMed

Takemoto, Yumi

2014-12-01

The thiol amino acid L-cysteine increases arterial blood pressure (ABP) when injected into the cerebrospinal fluid space in conscious rats, indicating a pressor response to centrally acting L-cysteine. A prior synaptic membrane binding assay suggests that L-cysteine has a strong affinity for the L-2-amino-4-phosphonobutyric acid (L-AP4) binding site. The central action of L-cysteine may be vial-AP4 sensitive receptors. The present study investigated cardiovascular responses to L-cysteine and L-ap4 microinjected into the autonomic area of the caudal ventrolateral medulla (CVLM) where inhibitory neurons regulate ABP via pre-sympathetic vasomotor neurons. Both the injection of L-cysteine and L-AP4 in the CVLM sites identified with L-glutamate produced the same depressor and bradycardic responses in urethane-anesthetized rats. Neither a prior antagonist microinjection of MK801 for the N-methyl-D-aspartate (NMDA) receptor nor CNQX for the non-NMDA receptor attenuated the responses to L-cysteine, but the combination of the two receptor blocking with an additional prior injection abolished the response. In contrast, either receptor blockade alone abolished the response to L-AP4, indicating distinct mechanisms between responses to L-cysteine and L-AP4 in the CVLM. The results indicate that the CVLM is a central active site for L-cysteine's cardiovascular response. Central L-cysteine's action could be independent of the L-AP4 sensitive receptors. Cardiovascular regulation may involve endogenous L-cysteine in the CVLM. Further multidisciplinary examinations are required to elaborate on L-cysteine's functional roles in the CVLM. Copyright © 2014 Elsevier B.V. All rights reserved.

Adaptive Laplacian filtering for sensorimotor rhythm-based brain-computer interfaces.

PubMed

Lu, Jun; McFarland, Dennis J; Wolpaw, Jonathan R

2013-02-01

Sensorimotor rhythms (SMRs) are 8-30 Hz oscillations in the electroencephalogram (EEG) recorded from the scalp over sensorimotor cortex that change with movement and/or movement imagery. Many brain-computer interface (BCI) studies have shown that people can learn to control SMR amplitudes and can use that control to move cursors and other objects in one, two or three dimensions. At the same time, if SMR-based BCIs are to be useful for people with neuromuscular disabilities, their accuracy and reliability must be improved substantially. These BCIs often use spatial filtering methods such as common average reference (CAR), Laplacian (LAP) filter or common spatial pattern (CSP) filter to enhance the signal-to-noise ratio of EEG. Here, we test the hypothesis that a new filter design, called an 'adaptive Laplacian (ALAP) filter', can provide better performance for SMR-based BCIs. An ALAP filter employs a Gaussian kernel to construct a smooth spatial gradient of channel weights and then simultaneously seeks the optimal kernel radius of this spatial filter and the regularization parameter of linear ridge regression. This optimization is based on minimizing the leave-one-out cross-validation error through a gradient descent method and is computationally feasible. Using a variety of kinds of BCI data from a total of 22 individuals, we compare the performances of ALAP filter to CAR, small LAP, large LAP and CSP filters. With a large number of channels and limited data, ALAP performs significantly better than CSP, CAR, small LAP and large LAP both in classification accuracy and in mean-squared error. Using fewer channels restricted to motor areas, ALAP is still superior to CAR, small LAP and large LAP, but equally matched to CSP. Thus, ALAP may help to improve the accuracy and robustness of SMR-based BCIs.

Combining FoxP3 and Helios with GARP/LAP markers can identify expanded Treg subsets in cancer patients.

PubMed

Abd Al Samid, May; Chaudhary, Belal; Khaled, Yazan S; Ammori, Basil J; Elkord, Eyad

2016-03-22

Regulatory T cells (Tregs) comprise numerous heterogeneous subsets with distinct phenotypic and functional features. Identifying Treg markers is critical to investigate the role and clinical impact of various Treg subsets in pathological settings, and also for developing more effective immunotherapies. We have recently shown that non-activated FoxP3-Helios+ and activated FoxP3+/-Helios+ CD4+ T cells express GARP/LAP immunosuppressive markers in healthy donors. In this study we report similar observations in the peripheral blood of patients with pancreatic cancer (PC) and liver metastases from colorectal cancer (LICRC). Comparing levels of different Treg subpopulations in cancer patients and controls, we report that in PC patients, and unlike LICRC patients, there was no increase in Treg levels as defined by FoxP3 and Helios. However, defining Tregs based on GARP/LAP expression showed that FoxP3-LAP+ Tregs in non-activated and activated settings, and FoxP3+Helios+GARP+LAP+ activated Tregs were significantly increased in both groups of patients, compared with controls. This work implies that a combination of Treg-specific markers could be used to more accurately determine expanded Treg subsets and to understand their contribution in cancer settings. Additionally, GARP-/+LAP+ CD4+ T cells made IL-10, and not IFN-γ, and levels of IL-10-secreting CD4+ T cells were elevated in LICRC patients, especially with higher tumor staging. Taken together, our results indicate that investigations of Treg levels in different cancers should consider diverse Treg-related markers such as GARP, LAP, Helios, and others and not only FoxP3 as a sole Treg-specific marker.

External validation of the fatty liver index and lipid accumulation product indices, using 1H-magnetic resonance spectroscopy, to identify hepatic steatosis in healthy controls and obese, insulin-resistant individuals.

PubMed

Cuthbertson, Daniel J; Weickert, Martin O; Lythgoe, Daniel; Sprung, Victoria S; Dobson, Rebecca; Shoajee-Moradie, Fariba; Umpleby, Margot; Pfeiffer, Andreas F H; Thomas, E Louise; Bell, Jimmy D; Jones, Helen; Kemp, Graham J

2014-11-01

Simple clinical algorithms including the fatty liver index (FLI) and lipid accumulation product (LAP) have been developed as surrogate markers for non-alcoholic fatty liver disease (NAFLD), constructed using (semi-quantitative) ultrasonography. This study aimed to validate FLI and LAP as measures of hepatic steatosis, as determined quantitatively by proton magnetic resonance spectroscopy (1H-MRS). Data were collected from 168 patients with NAFLD and 168 controls who had undergone clinical, biochemical and anthropometric assessment. Values of FLI and LAP were determined and assessed both as predictors of the presence of hepatic steatosis (liver fat>5.5%) and of actual liver fat content, as measured by 1H-MRS. The discriminative ability of FLI and LAP was estimated using the area under the receiver operator characteristic curve (AUROC). As FLI can also be interpreted as a predictive probability of hepatic steatosis, we assessed how well calibrated it was in our cohort. Linear regression with prediction intervals was used to assess the ability of FLI and LAP to predict liver fat content. Further validation was provided in 54 patients with type 2 diabetes mellitus. FLI, LAP and alanine transferase discriminated between patients with and without steatosis with an AUROC of 0.79 (IQR=0.74, 0.84), 0.78 (IQR=0.72, 0.83) and 0.83 (IQR=0.79, 0.88) respectively although could not quantitatively predict liver fat. Additionally, the algorithms accurately matched the observed percentages of patients with hepatic steatosis in our cohort. FLI and LAP may be used to identify patients with hepatic steatosis clinically or for research purposes but could not predict liver fat content. © 2014 European Society of Endocrinology.

Adaptive Laplacian filtering for sensorimotor rhythm-based brain-computer interfaces

NASA Astrophysics Data System (ADS)

Lu, Jun; McFarland, Dennis J.; Wolpaw, Jonathan R.

2013-02-01

Objective. Sensorimotor rhythms (SMRs) are 8-30 Hz oscillations in the electroencephalogram (EEG) recorded from the scalp over sensorimotor cortex that change with movement and/or movement imagery. Many brain-computer interface (BCI) studies have shown that people can learn to control SMR amplitudes and can use that control to move cursors and other objects in one, two or three dimensions. At the same time, if SMR-based BCIs are to be useful for people with neuromuscular disabilities, their accuracy and reliability must be improved substantially. These BCIs often use spatial filtering methods such as common average reference (CAR), Laplacian (LAP) filter or common spatial pattern (CSP) filter to enhance the signal-to-noise ratio of EEG. Here, we test the hypothesis that a new filter design, called an ‘adaptive Laplacian (ALAP) filter’, can provide better performance for SMR-based BCIs. Approach. An ALAP filter employs a Gaussian kernel to construct a smooth spatial gradient of channel weights and then simultaneously seeks the optimal kernel radius of this spatial filter and the regularization parameter of linear ridge regression. This optimization is based on minimizing the leave-one-out cross-validation error through a gradient descent method and is computationally feasible. Main results. Using a variety of kinds of BCI data from a total of 22 individuals, we compare the performances of ALAP filter to CAR, small LAP, large LAP and CSP filters. With a large number of channels and limited data, ALAP performs significantly better than CSP, CAR, small LAP and large LAP both in classification accuracy and in mean-squared error. Using fewer channels restricted to motor areas, ALAP is still superior to CAR, small LAP and large LAP, but equally matched to CSP. Significance. Thus, ALAP may help to improve the accuracy and robustness of SMR-based BCIs.

Tokyo Guidelines 2018: flowchart for the management of acute cholecystitis.

PubMed

Okamoto, Kohji; Suzuki, Kenji; Takada, Tadahiro; Strasberg, Steven M; Asbun, Horacio J; Endo, Itaru; Iwashita, Yukio; Hibi, Taizo; Pitt, Henry A; Umezawa, Akiko; Asai, Koji; Han, Ho-Seong; Hwang, Tsann-Long; Mori, Yasuhisa; Yoon, Yoo-Seok; Huang, Wayne Shih-Wei; Belli, Giulio; Dervenis, Christos; Yokoe, Masamichi; Kiriyama, Seiki; Itoi, Takao; Jagannath, Palepu; Garden, O James; Miura, Fumihiko; Nakamura, Masafumi; Horiguchi, Akihiko; Wakabayashi, Go; Cherqui, Daniel; de Santibañes, Eduardo; Shikata, Satoru; Noguchi, Yoshinori; Ukai, Tomohiko; Higuchi, Ryota; Wada, Keita; Honda, Goro; Supe, Avinash Nivritti; Yoshida, Masahiro; Mayumi, Toshihiko; Gouma, Dirk J; Deziel, Daniel J; Liau, Kui-Hin; Chen, Miin-Fu; Shibao, Kazunori; Liu, Keng-Hao; Su, Cheng-Hsi; Chan, Angus C W; Yoon, Dong-Sup; Choi, In-Seok; Jonas, Eduard; Chen, Xiao-Ping; Fan, Sheung Tat; Ker, Chen-Guo; Giménez, Mariano Eduardo; Kitano, Seigo; Inomata, Masafumi; Hirata, Koichi; Inui, Kazuo; Sumiyama, Yoshinobu; Yamamoto, Masakazu

2018-01-01

We propose a new flowchart for the treatment of acute cholecystitis (AC) in the Tokyo Guidelines 2018 (TG18). Grade III AC was not indicated for straightforward laparoscopic cholecystectomy (Lap-C). Following analysis of subsequent clinical investigations and drawing on Big Data in particular, TG18 proposes that some Grade III AC can be treated by Lap-C when performed at advanced centers with specialized surgeons experienced in this procedure and for patients that satisfy certain strict criteria. For Grade I, TG18 recommends early Lap-C if the patients meet the criteria of Charlson comorbidity index (CCI) ≤5 and American Society of Anesthesiologists physical status classification (ASA-PS) ≤2. For Grade II AC, if patients meet the criteria of CCI ≤5 and ASA-PS ≤2, TG18 recommends early Lap-C performed by experienced surgeons; and if not, after medical treatment and/or gallbladder drainage, Lap-C would be indicated. TG18 proposes that Lap-C is indicated in Grade III patients with strict criteria. These are that the patients have favorable organ system failure, and negative predictive factors, who meet the criteria of CCI ≤3 and ASA-PS ≤2 and who are being treated at an advanced center (where experienced surgeons practice). If the patient is not considered suitable for early surgery, TG18 recommends early/urgent biliary drainage followed by delayed Lap-C once the patient's overall condition has improved. Free full articles and mobile app of TG18 are available at: http://www.jshbps.jp/modules/en/index.php?content_id=47. Related clinical questions and references are also included. © 2017 Japanese Society of Hepato-Biliary-Pancreatic Surgery.

Pulmonary venous flow determinants of left atrial pressure under different loading conditions in a chronic animal model with mitral regurgitation

NASA Technical Reports Server (NTRS)

Yang, Hua; Jones, Michael; Shiota, Takahiro; Qin, Jian Xin; Kim, Yong Jin; Popovic, Zoran B.; Pu, Min; Greenberg, Neil L.; Cardon, Lisa A.; Eto, Yoko;

The effect of furosemide on left atrial pressure in dogs with mitral valve regurgitation.

PubMed

Suzuki, S; Ishikawa, T; Hamabe, L; Aytemiz, D; Huai-Che, H; Fukushima, R; Machida, N; Tanaka, R

2011-01-01

The effects of furosemide on left atrial pressure (LAP) in dogs with mitral regurgitation (MR) have not been documented in a quantitative manner and between different routes of administration. To document LAP and echocardiographic parameters in MR dogs administered furosemide IV or PO, in order to document changes in LAP after furosemide treatment. Five healthy Beagle dogs (3 males and 2 females; aged 2 years) were used. Experimental, cross-over, and interventional study. LAP was measured before the administration of furosemide, and 30 minutes, 1, 1.5, 2, 3, 4, 5, 6, 8, 12, and 24 hours after administration. Furosemide 1, 2, or 4 mg/kg IV, PO or placebo was administered. LAP was significantly decreased with all administrations of furosemide but not after placebo (P < .05, respectively). The max reduction was observed 1 hour (1 mg/kg IV, 15.04 ± 7.02 mmHg), 3 hours (2, 4 mg/kg IV, 13.28 ± 8.01, 9.23 ± 4.92 mmHg), 4 hours (1 mg/kg PO, 14.68 ± 11.51 mmHg), and 5 hours (2, 4 mg/kg PO, 13.19 ± 10.52, 10.70 ± 7.69 mmHg). E wave and E/Ea were significantly decreased corresponding to the reduction of LAP after administration of 2 and 4 mg/kg (P < .05, respectively). LAP was decreased in proportion to the dosage of furosemide, which did not significantly differ between IV and PO of the same dosages. E wave and E/Ea might be useful for the treatment evaluation of furosemide. Copyright © 2011 by the American College of Veterinary Internal Medicine.

The effect of angiotensin-converting enzyme inhibitors of left atrial pressure in dogs with mitral valve regurgitation.

PubMed

Ishikawa, T; Tanaka, R; Suzuki, S; Miyaishi, Y; Akagi, H; Iino, Y; Fukushima, R; Yamane, Y

2010-01-01

Despite many epidemiological reports concerning the efficacy of angiotensin-converting enzyme (ACE) inhibitors in dogs with mitral regurgitation (MR), the hemodynamic effects of ACE inhibitor administration have not been fully evaluated. To document left atrial pressure (LAP) in dogs with MR administered ACE inhibitors, in order to obtain interesting information about daily LAP changes with administration of ACE inhibitors. Five healthy Beagle dogs weighing 9.8 to 14.2 kg (2 males and 3 females; aged 2 years). Experimental, crossover, and interventional study. Chordae tendineae rupture was induced, and a radiotelemetry transmitter catheter was inserted into the left atrium. LAP was recorded for 72 consecutive hours during which each of 3 ACE inhibitors--nalapril (0.5 mg/kg/d), temocapril (0.1 mg/kg/d), and alacepril (3.0 mg/kg/d)--were administered in a crossover study. Averaged diurnal LAP was significantly, but slightly reduced by alacepril (P = .03, 19.03 +/- 3.01-18.24 +/- 3.07 mmHg). The nightly drops in LAP caused by alacepril and enalapril were significantly higher than the daily drops (P = .03, -0.98 +/- 0.19 to -0.07 +/- 0.25 mmHg, and P = .03, -0.54 +/- 0.21-0.02 +/- 0.17 mmHg, respectively), despite the fact that the oral administrations were given in the morning. Systolic blood pressure (122.7 +/- 14.4-117.4 +/- 13.1 mmHg, P = .04) and systemic vascular resistance (5800 +/- 2685-5144 +/- 2077 dyne x s/cm5, P = .03) were decreased by ACE inhibitors. ACE inhibitors decrease LAP minimally, despite reductions in left ventricular afterload. ACE inhibitors should not be used to decrease LAP.

Lenslet array processors.

PubMed

Glaser, I

1982-04-01

By combining a lenslet array with masks it is possible to obtain a noncoherent optical processor capable of computing in parallel generalized 2-D discrete linear transformations. We present here an analysis of such lenslet array processors (LAP). The effect of several errors, including optical aberrations, diffraction, vignetting, and geometrical and mask errors, are calculated, and guidelines to optical design of LAP are derived. Using these results, both ultimate and practical performances of LAP are compared with those of competing techniques.

Light-Addressable Potentiometric Sensors using ZnO Nanorods as the Sensor Substrate for Bioanalytical Applications.

PubMed

Tu, Ying; Ahmad, Norlaily; Briscoe, Joe; Zhang, De-Wen; Krause, Steffi

2018-06-22

Light-addressable potentiometric sensors (LAPS) are of great interest in bioimaging applications such as the monitoring of concentrations in microfluidic channels or the investigation of metabolic and signaling events in living cells. By measuring the photocurrents at electrolyte-insulator-semiconductor (EIS) and electrolyte-semiconductor structures, LAPS can produce spatiotemporal images of chemical or biological analytes, electrical potentials and impedance. However, its commercial applications are often restricted by their limited AC photocurrents and resolution of LAPS images. Herein, for the first time, the use of 1D semiconducting oxides in the form of ZnO nanorods for LAPS imaging is explored to solve this issue. A significantly increased AC photocurrent with enhanced image resolution has been achieved based on ZnO nanorods, with a photocurrent of 45.7 ± 0.1 nA at a light intensity of 0.05 mW, a lateral resolution as low as 3.0 μm as demonstrated by images of a PMMA dot on ZnO nanorods and a pH sensitivity of 53 mV/pH. The suitability of the device for bioanalysis and bioimaging was demonstrated by monitoring the degradation of a thin poly(ester amide) film with the enzyme α-chymotrypsin using LAPS. This simple and robust route to fabricate LAPS substrates with excellent performance would provide tremendous opportunities for bioimaging.

Assessment of vitamin D status and serum CrossLaps levels in adults with primary lactose malabsorption.

PubMed

Enko, D; Kriegshäuser, G; Stolba, R; Mangge, H; Brandstetter, D; Mayr, N; Forstner, T; Halwachs-Baumann, G

2016-09-01

Primary adult-type lactose malabsorption (PALM) is a widespread inherited autosomal recessive condition, which is considered to be associated with osteoporosis. This prospective study aimed at assessing the 25-hydroxy-vitamin D (25(OH)D) status and serum CrossLaps levels in individuals with PALM and normal controls. All participants (n=210) underwent genotyping for the LCT C/T-13910 polymorphism, 25(OH)D and CrossLaps measurements and clinical examinations. In addition, the anthropometric data (that is, height, weight and body mass index) were determined. Fifty-five individuals with PALM (that is, LCT C/C-13910 homozygotes) showed lower 25(OH)D (mean: 24.95±10.04 vs 28.59±9.56 ng/ml, P=0.018) and higher CrossLaps serum levels (mean: 0.46±0.31 vs 0.43±0.49 ng/ml, P=0.251) compared with 155 normal controls (that is, LCT C/T-13910 hetero- or T/T-13910 homozygotes). Anthropometric data were similar between PALM probands and controls. Individuals with PALM were found to have lower 25(OH)D and higher CrossLaps serum levels compared with normal controls. In order to preserve life-long bone health, routine 25(OH)D and CrossLaps serum measurements should be performed in individuals with PALM.

Large-scale Advanced Prop-fan (LAP) high speed wind tunnel test report

NASA Technical Reports Server (NTRS)

Campbell, William A.; Wainauski, Harold S.; Arseneaux, Peter J.

1988-01-01

High Speed Wind Tunnel testing of the SR-7L Large Scale Advanced Prop-Fan (LAP) is reported. The LAP is a 2.74 meter (9.0 ft) diameter, 8-bladed tractor type rated for 4475 KW (6000 SHP) at 1698 rpm. It was designated and built by Hamilton Standard under contract to the NASA Lewis Research Center. The LAP employs thin swept blades to provide efficient propulsion at flight speeds up to Mach .85. Testing was conducted in the ONERA S1-MA Atmospheric Wind Tunnel in Modane, France. The test objectives were to confirm that the LAP is free from high speed classical flutter, determine the structural and aerodynamic response to angular inflow, measure blade surface pressures (static and dynamic) and evaluate the aerodynamic performance at various blade angles, rotational speeds and Mach numbers. The measured structural and aerodynamic performance of the LAP correlated well with analytical predictions thereby providing confidence in the computer prediction codes used for the design. There were no signs of classical flutter throughout all phases of the test up to and including the 0.84 maximum Mach number achieved. Steady and unsteady blade surface pressures were successfully measured for a wide range of Mach numbers, inflow angles, rotational speeds and blade angles. No barriers were discovered that would prevent proceeding with the PTA (Prop-Fan Test Assessment) Flight Test Program scheduled for early 1987.

Betel-quid and alcohol use were associated with lipid accumulation product among male factory workers in Taiwan.

PubMed

Huang, Chih-Fang; Chen, Chao-Tung; Wang, Pei-Ming; Koo, Malcolm

2015-05-01

In this study, cardiometabolic risk associated with betel-quid, alcohol and cigarette use, based on a simple index-lipid accumulation product (LAP), was investigated in Taiwanese male factory workers. Male factory workers were recruited during their annual routine health examination at a hospital in south Taiwan. The risk of cardiometabolic disorders was estimated by the use of LAP, calculated as (waist circumference [cm]-65)×(triglyceride concentration [mmol/l]). Multiple linear regression analyses were conducted to assess the risk factors of natural logarithm-transformed LAP. Of the 815 participants, 40% (325/815) were current alcohol users, 30% (248/815) were current smokers and 7% (53/815) were current betel-quid users. Current betel-quid use, alcohol use, older age, lack of exercise and higher body mass index were found to be significant and independent factors associated with natural logarithm-transformed LAP. Betel-quid and alcohol, but not cigarette use, were independent risk factors of logarithm-transformed LAP, adjusting for age, exercise and body mass index in male Taiwanese factory workers. LAP can be considered as a simple and useful method for screening of cardiometabolic risk. © The Author 2014. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Laparoscopic virtual reality simulator and box trainer in gynecology.

PubMed

Akdemir, Ali; Sendağ, Fatih; Oztekin, Mehmet K

2014-05-01

To investigate whether a virtual reality simulator (LapSim) and traditional box trainer are effective tools for the acquisition of basic laparoscopic skills, and whether the LapSim is superior to the box trainer in surgical education. In a study at Ege University School of Medicine, Izmir, Turkey, between September 2008 and March 2013, 40 first- and second-year residents were randomized to train via the LapSim or box trainer for 4 weeks, and 20 senior residents were allocated to a control group. All 3 groups performed laparoscopic bilateral tubal ligation. Video records of each operation were assessed via the general rating scale of the Objective Structured Assessment of Laparoscopic Salpingectomy and by operation time in seconds. Compared with the control group, the LapSim and box trainer groups performed significantly better in total score (P<0.01 and P<0.01, respectively) and time (P=0.03 and P=0.01, respectively). There were no differences between the LapSim and box trainer groups. Novice residents who trained on a LapSim or box trainer performed better live laparoscopies than residents who trained via standard clinical surgical education. Training with a virtual reality simulator or box trainer should be considered before actual laparoscopic procedures are carried out. Copyright © 2014 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.

Leucine aminopeptidase blood test

MedlinePlus

Serum leucine aminopeptidase; LAP - serum ... Chernecky CC, Berger BJ. Leucine aminopeptidase (LAP) - blood. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures . 6th ed. St Louis, MO: Elsevier ...

Dynamic expression of the LAP family of genes during early development of Xenopus tropicalis.

PubMed

Yang, Qiutan; Lv, Xiaoyan; Kong, Qinghua; Li, Chaocui; Zhou, Qin; Mao, Bingyu

2011-10-01

The leucine-rich repeats and PDZ (LAP) family of genes are crucial for the maintenance of cell polarity as well as for epithelial homeostasis and tumor suppression in both vertebrates and invertebrates. Four members of this gene family are known: densin, erbin, scribble and lano. Here, we identified the four members of the LAP gene family in Xenopus tropicalis and studied their expression patterns during embryonic development. The Xenopus LAP proteins show a conserved domain structure that is similar to their homologs in other vertebrates. In Xenopus embryos, these genes were detected in animal cap cells at the early gastrula stage. At later stages of development, they were widely expressed in epithelial tissues that are highly polar in nature, including the neural epithelia, optic and otic vesicles, and in the pronephros. These data suggest that the roles of the Xenopus LAP genes in the control of cell polarity and morphogenesis are conserved during early development. Erbin and lano show similar expression patterns in the developing head, suggesting potential functional interactions between the two molecules in vivo.

Laparoscopic jejunostomy for obstructing upper gastrointestinal malignancies

PubMed Central

TSUJIMOTO, HIRONORI; HIRAKI, SHUICHI; TAKAHATA, RISA; NOMURA, SHINSUKE; ITO, NOZOMI; KANEMATSU, KYOHEI; HORIGUCHI, HIROYUKI; AOSASA, SUEFUMI; YAMAMOTO, JUNJI; HASE, KAZUO

2015-01-01

The aim of this study was to describe a minimally invasive laparoscopic jejunostomy (Lap-J) technique for obstruction due to upper gastrointestinal malignancies and evaluate the nutritional benefit of Lap-J during neoadjuvant chemotherapy (NAC) in cases with obstructing esophageal cancer. Under general anesthesia, the jejunum 20–30 cm distant from the Treitz ligament was pulled out through an extended umbilical laparoscopic incision and a jejunal tube was inserted to 30 cm. The loop of bowel was gently returned to the abdomen and the feeding tube was drawn through the abdominal wall via the left lower incision. The jejunum was then laparoscopically sutured to the anterior abdominal wall. Lap-J was performed in 26 cases. The median operative time was 82 min. The postoperative course was uneventful. Lap-J prior to NAC was not associated with a decrease in body weight or serum total protein during NAC, compared with patients who received NAC without Lap-J. This minimally invasive jejunostomy technique may be particularly useful in patients in whom endoscopic therapy is not feasible due to obstruction from upper gastrointestinal malignancies. PMID:26807238

Plastic deformation and failure mechanisms in nano-scale notched metallic glass specimens under tensile loading

NASA Astrophysics Data System (ADS)

Dutta, Tanmay; Chauniyal, Ashish; Singh, I.; Narasimhan, R.; Thamburaja, P.; Ramamurty, U.

2018-02-01

In this work, numerical simulations using molecular dynamics and non-local plasticity based finite element analysis are carried out on tensile loading of nano-scale double edge notched metallic glass specimens. The effect of acuteness of notches as well as the metallic glass chemical composition or internal material length scale on the plastic deformation response of the specimens are studied. Both MD and FE simulations, in spite of the fundamental differences in their nature, indicate near-identical deformation features. Results show two distinct transitions in the notch tip deformation behavior as the acuity is increased, first from single shear band dominant plastic flow localization to ligament necking, and then to double shear banding in notches that are very sharp. Specimens with moderately blunt notches and composition showing wider shear bands or higher material length scale characterizing the interaction stress associated with flow defects display profuse plastic deformation and failure by ligament necking. These results are rationalized from the role of the interaction stress and development of the notch root plastic zones.

Dependency of Shear Strength on Test Rate in SiC/BSAS Ceramic Matrix Composite at Elevated Temperature

NASA Technical Reports Server (NTRS)

Choi, Sung R.; Bansal, Narottam P.; Gyekenyesi, John P.

2003-01-01

Both interlaminar and in-plane shear strengths of a unidirectional Hi-Nicalon(TM) fiber-reinforced barium strontium aluminosilicate (SiC/BSAS) composite were determined at 1100 C in air as a function of test rate using double notch shear test specimens. The composite exhibited a significant effect of test rate on shear strength, regardless of orientation which was either in interlaminar or in in-plane direction, resulting in an appreciable shear-strength degradation of about 50 percent as test rate decreased from 3.3 10(exp -1) mm/s to 3.3 10(exp -5) mm/s. The rate dependency of composite's shear strength was very similar to that of ultimate tensile strength at 1100 C observed in a similar composite (2-D SiC/BSAS) in which tensile strength decreased by about 60 percent when test rate varied from the highest (5 MPa/s) to the lowest (0.005 MPa/s). A phenomenological, power-law slow crack growth formulation was proposed and formulated to account for the rate dependency of shear strength of the composite.

Study on active lap tool influence function in grinding 1.8 m primary mirror.

PubMed

Haitao, Liu; Zhige, Zeng; Fan, Wu; Bin, Fan; Yongjian, Wan

2013-11-01

We present a theoretical modeling method to predict the ring tool influence function (TIF) based on the computer-controlled active lap process. The gap on the lap-grinding layer is considered, and its influence on the ring TIF is analyzed too. The relationship between the shape of the ring TIF and the lap-workpiece rotation speed ratio is discussed in this paper. The recipe for calculating dwell time for axisymmetric fabrication is discussed. The grinding process of a 1.8 m primary mirror is improved based on these results. The grinding process is accomplished after 30 circles of grinding, and the surface shape error is from PV 82 μm RMS 16.4 μm reduced to PV 13.5 μm RMS 2.5 μm.

Review on failure prediction techniques of composite single lap joint

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ab Ghani, A.F., E-mail: ahmadfuad@utem.edu.my; Rivai, Ahmad, E-mail: ahmadrivai@utem.edu.my

2016-03-29

Adhesive bonding is the most appropriate joining method in construction of composite structures. The use of reliable design and prediction technique will produce better performance of bonded joints. Several papers from recent papers and journals have been reviewed and synthesized to understand the current state of the art in this area. It is done by studying the most relevant analytical solutions for composite adherends with start of reviewing the most fundamental ones involving beam/plate theory. It is then extended to review single lap joint non linearity and failure prediction and finally on the failure prediction on composite single lap joint.more » The review also encompasses the finite element modelling part as tool to predict the elastic response of composite single lap joint and failure prediction numerically.« less

Shear Strength and Interfacial Toughness Characterization of Sapphire-Epoxy Interfaces for Nacre-Inspired Composites.

PubMed

Behr, Sebastian; Jungblut, Laura; Swain, Michael V; Schneider, Gerold A

2016-10-12

The common tensile lap-shear test for adhesive joints is inappropriate for brittle substrates such as glasses or ceramics where stress intensifications due to clamping and additional bending moments invalidate results. Nevertheless, bonding of glasses and ceramics is still important in display applications for electronics, in safety glass and ballistic armor, for dental braces and restoratives, or in recently developed bioinspired composites. To mechanically characterize adhesive bondings in these fields nonetheless, a novel approach based on the so-called Schwickerath test for dental sintered joints is used. This new method not only matches data from conventional analysis but also uniquely combines the accurate determination of interfacial shear strength and toughness in one simple test. The approach is verified for sapphire-epoxy joints that are of interest for bioinspired composites. For these, the procedure not only provides quantitative interfacial properties for the first time, it also exemplarily suggests annealing of sapphire at 1000 °C for 10 h for mechanically and economically effective improvements of the interfacial bond strength and toughness. With increases of strength and toughness from approximately 8 to 29 MPa and from 2.6 to 35 J/m 2 , respectively, this thermal modification drastically enhances the properties of unmodified sapphire-epoxy interfaces. At the same time, it is much more convenient than wet-chemical approaches such as silanization. Hence, besides the introduction of a new testing procedure for adhesive joints of brittle or expensive substrates, a new and facile annealing process for improvements of the adhesive properties of sapphire is suggested and quantitative data for the mechanical properties of sapphire-epoxy interfaces that are common in synthetic nacre-inspired composites are provided for the first time.

On the Process-Related Rivet Microstructural Evolution, Material Flow and Mechanical Properties of Ti-6Al-4V/GFRP Friction-Riveted Joints

PubMed Central

Borba, Natascha Z.; Afonso, Conrado R. M.; Blaga, Lucian; dos Santos, Jorge F.; Canto, Leonardo B.; Amancio-Filho, Sergio T.

2017-01-01

In the current work, process-related thermo-mechanical changes in the rivet microstructure, joint local and global mechanical properties, and their correlation with the rivet plastic deformation regime were investigated for Ti-6Al-4V (rivet) and glass-fiber-reinforced polyester (GF-P) friction-riveted joints of a single polymeric base plate. Joints displaying similar quasi-static mechanical performance to conventional bolted joints were selected for detailed characterization. The mechanical performance was assessed on lap shear specimens, whereby the friction-riveted joints were connected with AA2198 gussets. Two levels of energy input were used, resulting in process temperatures varying from 460 ± 130 °C to 758 ± 56 °C and fast cooling rates (178 ± 15 °C/s, 59 ± 15 °C/s). A complex final microstructure was identified in the rivet. Whereas equiaxial α-grains with β-phase precipitated in their grain boundaries were identified in the rivet heat-affected zone, refined α′ martensite, Widmanstätten structures and β-fleck domains were present in the plastically deformed rivet volume. The transition from equiaxed to acicular structures resulted in an increase of up to 24% in microhardness in comparison to the base material. A study on the rivet material flow through microtexture of the α-Ti phase and β-fleck orientation revealed a strong effect of shear stress and forging which induced simple shear deformation. By combining advanced microstructural analysis techniques with local mechanical testing and temperature measurement, the nature of the complex rivet plastic deformational regime could be determined. PMID:28772545

On the water lapping of felines and the water running of lizards

PubMed Central

Aristoff, Jeffrey M; Stocker, Roman; Reis, Pedro M

2011-01-01

We consider two biological phenomena taking place at the air-water interface: the water lapping of felines and the water running of lizards. Although seemingly disparate motions, we show that they are intimately linked by their underlying hydrodynamics and belong to a broader class of processes called Froude mechanisms. We describe how both felines and lizards exploit inertia to defeat gravity, and discuss water lapping and water running in the broader context of water exit and water entry, respectively. PMID:21655444

Habit modification of potassium acid phthalate (KAP) single crystals by impurities

NASA Astrophysics Data System (ADS)

Murugakoothan, P.; Mohan Kumar, R.; Ushasree, P. M.; Jayavel, R.; Dhanasekaran, R.; Ramasamy, P.

1999-12-01

Nonlinear optical materials potassium dihydrogen phosphate (KDP), urea and L-arginine phosphate (LAP)-doped KAP crystals were grown by the slow cooling method. The LAP-doped crystals show pronounced habit modification compared to KDP and urea doping. The effect of these impurities on growth kinetics, surface morphology, habit modification, structure, optical and mechanical properties have been studied. Among the three impurities, urea doping yields high mechanical stability and optical transmission and for KDP and LAP doping there is a decrease in optical transmission.

A new and specific non-NMDA receptor antagonist, FG 9065, blocks L-AP4-evoked depolarization in rat cerebral cortex.

PubMed

Sheardown, M J

1988-04-13

L(+)-AP4 (2-amino-4-phosphonobutyrate) depolarized slices of rat cerebral cortex, when applied following a 2 min priming application of quisqualate. This response diminishes with time and is not seen after NMDA application. A new selective non-N-methyl-D-aspartate (NMDA) antagonist, 6-cyano-7-nitro-2,3-dihydroxyquinoxaline (FG 9065), inhibits the L(+)-AP4 depolarization. It is argued that the response is mediated indirectly by postsynaptic quisqualate receptors.

Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) with Raman Imaging Applied to Lunar Meteorites.

PubMed

Smith, Joseph P; Smith, Frank C; Booksh, Karl S

2018-03-01

Lunar meteorites provide a more random sampling of the surface of the Moon than do the returned lunar samples, and they provide valuable information to help estimate the chemical composition of the lunar crust, the lunar mantle, and the bulk Moon. As of July 2014, ∼96 lunar meteorites had been documented and ten of these are unbrecciated mare basalts. Using Raman imaging with multivariate curve resolution-alternating least squares (MCR-ALS), we investigated portions of polished thin sections of paired, unbrecciated, mare-basalt lunar meteorites that had been collected from the LaPaz Icefield (LAP) of Antarctica-LAP 02205 and LAP 04841. Polarized light microscopy displays that both meteorites are heterogeneous and consist of polydispersed sized and shaped particles of varying chemical composition. For two distinct probed areas within each meteorite, the individual chemical species and associated chemical maps were elucidated using MCR-ALS applied to Raman hyperspectral images. For LAP 02205, spatially and spectrally resolved clinopyroxene, ilmenite, substrate-adhesive epoxy, and diamond polish were observed within the probed areas. Similarly, for LAP 04841, spatially resolved chemical images with corresponding resolved Raman spectra of clinopyroxene, troilite, a high-temperature polymorph of anorthite, substrate-adhesive epoxy, and diamond polish were generated. In both LAP 02205 and LAP 04841, substrate-adhesive epoxy and diamond polish were more readily observed within fractures/veinlet features. Spectrally diverse clinopyroxenes were resolved in LAP 04841. Factors that allow these resolved clinopyroxenes to be differentiated include crystal orientation, spatially distinct chemical zoning of pyroxene crystals, and/or chemical and molecular composition. The minerals identified using this analytical methodology-clinopyroxene, anorthite, ilmenite, and troilite-are consistent with the results of previous studies of the two meteorites using electron microprobe analysis. To our knowledge, this is the first report of MCR-ALS with Raman imaging used for the investigation of both lunar and other types of meteorites. We have demonstrated the use of multivariate analysis methods, namely MCR-ALS, with Raman imaging to investigate heterogeneous lunar meteorites. Our analytical methodology can be used to elucidate the chemical, molecular, and structural characteristics of phases in a host of complex, heterogeneous geological, geochemical, and extraterrestrial materials.

Role of C/EBPβ-LAP and C/EBPβ-LIP in early adipogenic differentiation of human white adipose-derived progenitors and at later stages in immature adipocytes.

PubMed

Lechner, Stefan; Mitterberger, Maria C; Mattesich, Monika; Zwerschke, Werner

2013-01-01

We investigated the role of the major isoforms of CCAAT enhancer binding protein β (C/EBPβ), C/EBPβ-LAP and C/EBPβ-LIP, in adipogenesis of human white adipose-derived stromal/progenitor cells (ASC). C/EBPβ gene expression was transiently induced early in adipogenesis. At later stages, in immature adipocytes, the C/EBPβ mRNA and protein levels declined. The C/EBPβ-LIP protein steady-state level decreased considerably stronger than the C/EBPβ-LAP level and the C/EBPβ-LIP half-life was significantly shorter than the C/EBPβ-LAP half-life. The turn-over of both C/EBPβ-isoforms was regulated by ubiquitin/proteasome-dependent degradation. These data suggest that the protein stability of the C/EBPβ-isoforms is differentially regulated in the course of adipogenesis and in immature adipocytes. Constitutive overexpression of C/EBPβ-LIP had antiadipogenic activity in human ASC. C/EBPβ-LAP, which promotes adipogenesis in mouse 3T3-L1 preadipocytes by directly activating expression of the adipogenic keyregulator PPARγ2, induced the expression of PPARγ2 and of the adipocyte differentiation gene product FABP4 in confluent ASC in the absence of adipogenic hormones. At later stages after hormone cocktail-induced adipogenesis, in immature adipocytes, constitutive overexpression of C/EBPβ-LAP led to reduced expression of PPARγ2 and FABP4, C/EBPα expression was downregulated and the expression of the adipocyte differentiation gene products adiponectin and leptin was impaired. These findings suggest that constitutive overexpression of C/EBPβ-LAP induces adipogenesis in human ASC and negatively regulates the expression of adipogenic regulators and certain adipocyte differentiation gene products in immature adipocytes. We conclude the regulation of both C/EBPβ gene expression and C/EBPβ-LIP and C/EBPβ-LAP protein turn-over plays an important role for the expression of adipogenic regulators and/or adipocyte differentiation genes in early adipogenic differentiation of human ASC and at later stages in human immature adipocytes. Copyright © 2012 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.

Positive Correlation Between Motion Analysis Data on the LapMentor Virtual Reality Laparoscopic Surgical Simulator and the Results from Videotape Assessment of Real Laparoscopic Surgeries

PubMed Central

McDougall, Elspeth M.; Ono, Yoshinari; Hattori, Ryohei; Baba, Shiro; Iwamura, Masatsugu; Terachi, Toshiro; Naito, Seiji; Clayman, Ralph V.

2012-01-01

Abstract Purpose We studied the construct validity of the LapMentor, a virtual reality laparoscopic surgical simulator, and the correlation between the data collected on the LapMentor and the results of video assessment of real laparoscopic surgeries. Materials and Methods Ninety-two urologists were tested on basic skill tasks No. 3 (SK3) to No. 8 (SK8) on the LapMentor. They were divided into three groups: Group A (n=25) had no experience with laparoscopic surgeries as a chief surgeon; group B (n=33) had <35 experiences; and group C (n=34) had ≥35 experiences. Group scores on the accuracy, efficacy, and time of the tasks were compared. Forty physicians with ≥20 experiences supplied unedited videotapes showing a laparoscopic nephrectomy or an adrenalectomy in its entirety, and the videos were assessed in a blinded fashion by expert referees. Correlations between the videotape score (VS) and the performances on the LapMentor were analyzed. Results Group C showed significantly better outcomes than group A in the accuracy (SK5) (P=0.013), efficacy (SK8) (P=0.014), or speed (SKs 3 and 8) (P=0.009 and P=0.002, respectively) of the performances of LapMentor. Group B showed significantly better outcomes than group A in the speed and efficacy of the performances in SK8 (P=0.011 and P=0.029, respectively). Analyses of motion analysis data of LapMentor demonstrated that smooth and ideal movement of instruments is more important than speed of the movement of instruments to achieve accurate performances in each task. Multiple linear regression analysis indicated that the average score of the accuracy in SK4, 5, and 8 had significant positive correlation with VS (P=0.01). Conclusions This study demonstrated the construct and predictive validity of the LapMentor basic skill tasks, supporting their possible usefulness for the preclinical evaluation of laparoscopic skills. PMID:22642549

Friction stir welding joint of dissimilar materials between AZ31B magnesium and 6061 aluminum alloys: Microstructure studies and mechanical characterizations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mohammadi, J.; Behnamian, Y.; Mostafaei, A., E-mail: amir.mostafaei@gmail.com

2015-03-15

Friction stir welding is an efficient manufacturing method for joining dissimilar alloys, which can dramatically reduce grain sizes and offer high mechanical joint efficiency. Lap FSW joints between dissimilar AZ31B and Al 6061 alloy sheets were made at various tool rotation and travel speeds. Rotation and travel speeds varied between 560–1400 r/min and 16–40 mm/min respectively, where the ratio between these parameters was such that nearly constant pitch distances were applied during welding. X-ray diffraction pattern (XRD), optical microscopy images (OM), electron probe microanalysis (EPMA) and scanning electron microscopy equipped with an energy-dispersive X-ray spectroscopy (SEM-EDS) were used to investigatemore » the microstructures of the joints welded. Intermetallic phases including Al{sub 12}Mg{sub 17} (γ) and Al{sub 3}Mg{sub 2} (β) were detected in the weld zone (WZ). For different tool rotation speeds, the morphology of the microstructure in the stir zone changed significantly with travel speed. Lap shear tensile test results indicated that by simultaneously increasing the tool rotation and travel speeds to 1400 r/min and 40 mm/min, the joint tensile strength and ductility reached a maximum. Microhardness measurements and tensile stress–strain curves indicated that mechanical properties were affected by FSW parameters and mainly depended on the formation of intermetallic compounds in the weld zone. In addition, a debonding failure mode in the Al/Mg dissimilar weld nugget was investigated by SEM and surface fracture studies indicated that the presence of intermetallic compounds in the weld zone controlled the failure mode. XRD analysis of the fracture surface indicated the presence of brittle intermetallic compounds including Al{sub 12}Mg{sub 17} (γ) and Al{sub 3}Mg{sub 2} (β). - Highlights: • Dissimilar Al/Mg joint was obtained by lap friction stir welding technique. • Effect of rotation and travel speeds on the formation of intermetallic compounds • Microstructure and chemical studies including metallography, XRD, SEM-EDS, and EPMA • Mechanical property tests such as stress–strain curves, failure load and hardness • IMCs as Al{sub 3}Mg{sub 2} and Al{sub 12}Mg{sub 17} were identified in weld nugget and at Al/Mg interface.« less

Seismic moment tensor for anisotropic media: implication for Non-double-couple earthquakes

NASA Astrophysics Data System (ADS)

Cai, X.; Chen, X.; Chen, Y.; Cai, M.

2008-12-01

It is often found that the inversion results of seismic moment tensor from real seismic recorded data show the trace of seismic moment tensor M is not zero, a phenomenon called non-double-couple earthquake sources mechanism. Recently we have derived the analytical expressions of M in transversely isotropic media with the titled axis of symmetry and the results shows even only pure shear-motion of fault can lead to the implosive components determined by several combined anisotropic elastic constants. Many non-double-couple earthquakes from observations often appear in volcanic and geothermal areas (Julian, 1998), where there exist a mount of stress-aligned fluid-saturated parallel vertical micro-cracks identical to transversely isotropic media (Crampin, 2008), this stress-aligned crack will modify the seismic moment tensor. In another word, non-double-couple earthquakes don't mean to have a seismic failure movement perpendicular to the fault plane, while traditional research of seismic moment tensor focus on the case of isotropy, which cannot provide correct interpretation of seismic source mechanism. Reference: Julian, B.R., Miller, A.D. and Foulger, G.R., 1998. Non-double-couple earthquakes,1. Theory, Rev. Geophys., 36, 525¨C549. Crampin,S., Peacock,S., 2008, A review of the current understanding of seismic shear-wave splitting in the Earth's crust and common fallacies in interpretation, wave motion, 45,675-722

New generation fiber reinforced polymer composites incorporating carbon nanotubes

NASA Astrophysics Data System (ADS)

Soliman, Eslam

The last five decades observed an increasing use of fiber reinforced polymer (FRP) composites as alternative construction materials for aerospace and infrastructure. The high specific strength of FRP attracted its use as non-corrosive reinforcement. However, FRP materials were characterized with a relatively low ductility and low shear strength compared with steel reinforcement. On the other hand, carbon nanotubes (CNTs) have been introduced in the last decade as a material with minimal defect that is capable of increasing the mechanical properties of polymer matrices. This dissertation reports experimental investigations on the use of multi-walled carbon nanotubes (MWCNTs) to produce a new generation of FRP composites. The experiments showed significant improvements in the flexure properties of the nanocomposite when functionalized MWCNTs were used. In addition, MWCNTs were used to produce FRP composites in order to examine static, dynamic, and creep behavior. The MWCNTs improved the off-axis tension, off-axis flexure, FRP lap shear joint responses. In addition, they reduced the creep of FRP-concrete interface, enhanced the fracture toughness, and altered the impact resistance significantly. In general, the MWCNTs are found to affect the behaviour of the FRP composites when matrix failure dominates the behaviour. The improvement in the mechanical response with the addition of low contents of MWCNTs would benefit many industrial and military applications such as strengthening structures using FRP composites, composite pipelines, aircrafts, and armoured vehicles.

Numerical modelling in friction lap joining of aluminium alloy and carbon-fiber-reinforced-plastic sheets

NASA Astrophysics Data System (ADS)

Das, A.; Bang, H. S.; Bang, H. S.

2018-05-01

Multi-material combinations of aluminium alloy and carbon-fiber-reinforced-plastics (CFRP) have gained attention in automotive and aerospace industries to enhance fuel efficiency and strength-to-weight ratio of components. Various limitations of laser beam welding, adhesive bonding and mechanical fasteners make these processes inefficient to join metal and CFRP sheets. Friction lap joining is an alternative choice for the same. Comprehensive studies in friction lap joining of aluminium to CFRP sheets are essential and scare in the literature. The present work reports a combined theoretical and experimental study in joining of AA5052 and CFRP sheets using friction lap joining process. A three-dimensional finite element based heat transfer model is developed to compute the temperature fields and thermal cycles. The computed results are validated extensively with the corresponding experimentally measured results.

Simultaneous Pure Laparoscopic Resection of Primary Colorectal Cancer and Synchronous Liver Metastases: A Single Institution Experience with Propensity Score Matching Analysis.

PubMed

Ivanecz, Arpad; Krebs, Bojan; Stozer, Andraz; Jagric, Tomaz; Plahuta, Irena; Potrc, Stojan

2018-03-01

The aim of the study was to compare the outcome of pure laparoscopic and open simultaneous resection of both the primary colorectal cancer and synchronous colorectal liver metastases (SCLM). From 2000 to 2016 all patients treated by simultaneous resection were assessed for entry in this single center, clinically nonrandomized trial. A propensity score matching was used to compare the laparoscopic group (LAP) to open surgery group (OPEN). Primary endpoints were perioperative and oncologic outcomes. Secondary endpoints were overall survival (OS) and disease-free survival (DFS). Of the 82 patients identified who underwent simultaneous liver resection for SCLM, 10 patients underwent LAP. All these consecutive patients from LAP were matched to 10 comparable OPEN. LAP reduced the length of hospital stay (P = 0.044) and solid food oral intake was faster (P = 0.006) in this group. No patient undergoing the laparoscopic procedure experienced conversion to the open technique. No difference was observed in operative time, blood loss, transfusion rate, narcotics requirement, clinical risk score, resection margin, R0 resections rate, morbidity, mortality and incisional hernias rate. The two groups did not differ significantly in terms of the 3-year OS rate (90 vs. 75%; P = 0.842) and DFS rate (60 vs. 57%; P = 0.724). LAP reduced the length of hospital stay and offers faster solid food oral intake. Comparable oncologic and survival outcomes can be achieved. LAP is beneficial for well selected patients in high volume centers with appropriate expertise.

Material removal and surface figure during pad polishing of fused silica

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suratwala, T I; Feit, M D; Steele, W A

2009-05-04

The material removal and surface figure after ceria pad polishing of fused silica glass have been measured and analyzed as a function of kinematics, loading conditions, and polishing time. Also, the friction at the workpiece/lap interface, the slope of the workpiece relative to the lap plane, and lap viscoelastic properties have been measured and correlated to material removal. The results show that the relative velocity between the workpiece & lap (determined by the kinematics) and the pressure distribution determine the spatial and temporal material removal and hence the final surface figure of the workpiece. In the case where the appliedmore » loading and relative velocity distribution over the workpiece are spatially uniform, a significant non-uniform spatial material removal from the workpiece surface is observed. This is due to a non-uniform pressure distribution resulting from: (1) a moment caused by a pivot point and interface friction forces; (2) viscoelastic relaxation of the polyurethane lap; and (3) a physical workpiece/lap interface mismatch. Both the kinematics and these contributions to the pressure distribution are quantitatively described, and then combined to form a spatial and temporal Preston model & code for material removal (called Surface Figure or SurF{copyright}). The surface figure simulations are consistent with the experiment for a wide variety of polishing conditions. This study is an important step towards deterministic full-aperture polishing, which would allow optical glass fabrication to be performed in a more repeatable, less iterative, and hence more economical manner.« less

A newly developed oval-shaped port device (E•Z ACCESS Oval type) for use in reduced port surgery: initial clinical experiences with cholecystectomy.

PubMed

Shibao, Kazunori; Takagi, Tsuyoshi; Higure, Aiichiro; Yamaguchi, Koji

2013-09-01

We recently developed an oval-shaped E•Z Access device designed exclusively for use with the LAP PROTECTOR™ Oval type device (Hakko Co. Ltd., Tokyo, Japan). The transverse abdominal opening diameter made by round-shaped (Alexis® Wound Retractor, Applied Medical, Rancho Santa Margarita, CA; and LAP PROTECTOR™ Round type) and oval-shaped (LAP PROTECTOR™ Oval type) wound retractors was measured and compared in 5 patients with cholecystolithiasis. Each device was placed through a single 25-mm longitudinal umbilical incision, and the length of trocar separation was compared. LESS cholecystectomy was then performed using the oval-shaped E•Z ACCESS/LAP PROTECTOR™. The transverse abdominal opening diameter was maximized with the LAP PROTECTOR™ Oval type device. The average distance between the working-ports for the glove method, round-shaped, and oval-shaped E•Z ACCESS/LAP PROTECTOR™ devices in the 25-mm umbilical incisions were 20 ± 0.8 mm, 24 ± 1.5 mm, and 35 ± 0.8 mm, respectively. Wider trocar separation was achieved using the oval-shaped device, making the surgical procedures easier to perform. No perioperative port-related or surgical complications were observed. LESS cholecystectomy using the E•Z ACCESS Oval type device was found to be technically feasible. The Oval type device appears to allow for wider trocar separation, thereby reducing stress on the surgeon, ensuring patient safety, and providing cosmetic benefits.

Pacing profiles of senior men and women at the 2017 IAAF World Cross Country Championships.

PubMed

Hanley, Brian

2018-06-01

The purpose of this study was to analyse and compare pacing profiles of senior men and women competing in the 2017 World Cross Country Championships. Finishing and split times were collated for 118 men and 81 women competing over the newly introduced race distance of 10 km (five laps of approximately 2 km). Athletes were grouped according to finishing time, and changes in pace measured using lap times, except between Laps 1 and 2 because of a shorter first lap (times relative to the winner were used instead). Within both men's and women's races, groups slowed during the early stages, but then either sped up or maintained pace during the last lap. There were few differences between groups with regard to overall pacing profiles, or between sexes. The men's fast finish contrasted with slower finishes found in previous editions (over 12 km), and the degree to which women were slower than men (approximately 12%) was very similar to track racing and showed the decision to equalise the distances run by both sexes was sound. As in other distance events, athletes are recommended to try to achieve an even pace throughout, an approach that proved beneficial to both gold medallists.

The Fundamental Reasons Why Laptop Computers should not be Used on Your Lap.

PubMed

Mortazavi, S A R; Taeb, S; Mortazavi, S M J; Zarei, S; Haghani, M; Habibzadeh, P; Shojaei-Fard, M B

2016-12-01

As a tendency to use new technologies, gadgets such as laptop computers are becoming more popular among students, teachers, businessmen and office workers. Today laptops are a great tool for education and learning, work and personal multimedia. Millions of men, especially those in the reproductive age, are frequently using their laptop computers on the lap (thigh). Over the past several years, our lab has focused on the health effects of exposure to different sources of electromagnetic fields such as cellular phones, mobile base stations, mobile phone jammers, laptop computers, radars, dentistry cavitrons and Magnetic Resonance Imaging (MRI). Our own studies as well as the studies performed by other researchers indicate that using laptop computers on the lap adversely affects the male reproductive health. When it is placed on the lap, not only the heat from a laptop computer can warm men's scrotums, the electromagnetic fields generated by laptop's internal electronic circuits as well as the Wi-Fi Radiofrequency radiation hazards (in a Wi-Fi connected laptop) may decrease sperm quality. Furthermore, due to poor working posture, laptops should not be used on the lap for long hours.

Calcium sequestration by fungal melanin inhibits calcium-calmodulin signalling to prevent LC3-associated phagocytosis.

PubMed

Kyrmizi, Irene; Ferreira, Helena; Carvalho, Agostinho; Figueroa, Julio Alberto Landero; Zarmpas, Pavlos; Cunha, Cristina; Akoumianaki, Tonia; Stylianou, Kostas; Deepe, George S; Samonis, George; Lacerda, João F; Campos, António; Kontoyiannis, Dimitrios P; Mihalopoulos, Nikolaos; Kwon-Chung, Kyung J; El-Benna, Jamel; Valsecchi, Isabel; Beauvais, Anne; Brakhage, Axel A; Neves, Nuno M; Latge, Jean-Paul; Chamilos, Georgios

2018-05-30

LC3-associated phagocytosis (LAP) is a non-canonical autophagy pathway regulated by Rubicon, with an emerging role in immune homeostasis and antifungal host defence. Aspergillus cell wall melanin protects conidia (spores) from killing by phagocytes and promotes pathogenicity through blocking nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-dependent activation of LAP. However, the signalling regulating LAP upstream of Rubicon and the mechanism of melanin-induced inhibition of this pathway remain incompletely understood. Herein, we identify a Ca 2+ signalling pathway that depends on intracellular Ca 2+ sources from endoplasmic reticulum, endoplasmic reticulum-phagosome communication, Ca 2+ release from phagosome lumen and calmodulin (CaM) recruitment, as a master regulator of Rubicon, the phagocyte NADPH oxidase NOX2 and other molecular components of LAP. Furthermore, we provide genetic evidence for the physiological importance of Ca 2+ -CaM signalling in aspergillosis. Finally, we demonstrate that Ca 2+ sequestration by Aspergillus melanin inside the phagosome abrogates activation of Ca 2+ -CaM signalling to inhibit LAP. These findings reveal the important role of Ca 2+ -CaM signalling in antifungal immunity and identify an immunological function of Ca 2+ binding by melanin pigments with broad physiological implications beyond fungal disease pathogenesis.

Dual-band infrared imaging for quantitative corrosion detection in aging aircraft

DOE Office of Scientific and Technical Information (OSTI.GOV)

Del Grande, N.K.

1993-12-31

Aircraft skin thickness-loss from corrosion has been measured using dual-band infrared (DBIR) imaging on a flash-heated Boeing 737 fuselage structure. The authors mapped surface temperature differences of 0.2 to 0.6 C for 5 to 14 % thickness losses within corroded lap splices at 0.4 seconds after the heat flash. The procedure mapped surface temperature differences at sites without surface-emissivity clutter (from dirt, dents, tape, markings, ink, sealants, uneven paint, paint stripper, exposed metal and roughness variations). They established the correlation of percent thickness loss with surface temperature rise using a partially corroded F-18 wing box and several aluminum panels whichmore » had thickness losses from milled flat-bottom holes. The authors mapped the lap splice composite thermal inertia, (k{rho}c){sup 1/2}, which characterized shallow skin defects within the lap splice at early times (<0.3 s) and deeper skin defects within the lap splice at late times (>0.4 s). Corrosion invaded the inside of the Boeing 737 lap splice, beneath the galley and the latrine, where they observed ``pillowing`` from volume build-up of corrosion by-products.« less

Dual-band infrared imaging for quantitative corrosion detection in aging aircraft

DOE Office of Scientific and Technical Information (OSTI.GOV)

Del Grande, N.K.

1993-11-01

Aircraft skin thickness-loss from corrosion has been measured using dual-band infrared (DBIR) imaging on a flash-heated Boeing 737 fuselage structure. We mapped surface temperature differences of 0.2 to 0.6 {degrees}C for 5 to 14% thickness losses within corroded lap splices at 0.4 seconds after the heat flash. Our procedure mapped surface temperature differences at sites without surface-emissivity clutter (from dirt, dents, tape, markings, ink, sealants, uneven paint, paint stripper, exposed metal and roughness variations). We established the correlation of percent thickness loss with surface temperature rise using a partially corroded F-18 wing box and several aluminum panels which had thicknessmore » losses from milled flat-bottom holes. We mapped the lap splice composite thermal inertia, (kpc){sup {1/2}}, which characterized shallow skin defects within the lap splice at early times (<0.3 s) and deeper skin defects within the lap splice at late times (>0.4 s). Corrosion invaded the inside of the Boeing 737 lap splice, beneath the galley and the latrine, where we observed ``pillowing`` from volume build-up of corrosion by-products.« less

The Fundamental Reasons Why Laptop Computers should not be Used on Your Lap

PubMed Central

Mortazavi, S.A.R.; Taeb, S.; Mortazavi, S.M.J.; Zarei, S.; Haghani, M.; Habibzadeh, P.; Shojaei-fard, M.B.

2016-01-01

As a tendency to use new technologies, gadgets such as laptop computers are becoming more popular among students, teachers, businessmen and office workers. Today laptops are a great tool for education and learning, work and personal multimedia. Millions of men, especially those in the reproductive age, are frequently using their laptop computers on the lap (thigh). Over the past several years, our lab has focused on the health effects of exposure to different sources of electromagnetic fields such as cellular phones, mobile base stations, mobile phone jammers, laptop computers, radars, dentistry cavitrons and Magnetic Resonance Imaging (MRI). Our own studies as well as the studies performed by other researchers indicate that using laptop computers on the lap adversely affects the male reproductive health. When it is placed on the lap, not only the heat from a laptop computer can warm men’s scrotums, the electromagnetic fields generated by laptop’s internal electronic circuits as well as the Wi-Fi Radiofrequency radiation hazards (in a Wi-Fi connected laptop) may decrease sperm quality. Furthermore, due to poor working posture, laptops should not be used on the lap for long hours. PMID:28144597

A stationary bulk planar ideal flow solution for the double shearing model

NASA Astrophysics Data System (ADS)

Lyamina, E. A.; Kalenova, N. V.; Date, P. P.

2018-04-01

This paper provides a general ideal flow solution for the double shearing model of pressure-dependent plasticity. This new solution is restricted to a special class of stationary planar flows. A distinguished feature of this class of solutions is that one family of characteristic lines is straight. The solution is analytic. The mapping between Cartesian and principal lines based coordinate systems is given in parametric form with characteristic coordinates being the parameters. A simple relation that connects the scale factor for one family of coordinate curves of the principal lines based coordinate system and the magnitude of velocity is derived. The original ideal flow theory is widely used as the basis for inverse methods for the preliminary design of metal forming processes driven by minimum plastic work. The new theory extends this area of application to granular materials.

Ideal flow theory for the double - shearing model as a basis for metal forming design

NASA Astrophysics Data System (ADS)

Alexandrov, S.; Trung, N. T.

2018-02-01

In the case of Tresca’ solids (i.e. solids obeying the Tresca yield criterion and its associated flow rule) ideal flows have been defined elsewhere as solenoidal smooth deformations in which an eigenvector field associated everywhere with the greatest principal stress (and strain rate) is fixed in the material. Under such conditions all material elements undergo paths of minimum plastic work, a condition which is often advantageous for metal forming processes. Therefore, the ideal flow theory is used as the basis of a procedure for the preliminary design of such processes. The present paper extends the theory of stationary planar ideal flow to pressure dependent materials obeying the double shearing model and the double slip and rotation model. It is shown that the original problem of plasticity reduces to a purely geometric problem. The corresponding system of equations is hyperbolic. The characteristic relations are integrated in elementary functions. In regions where one family of characteristics is straight, mapping between the principal lines and Cartesian coordinates is determined by linear ordinary differential equations. An illustrative example is provided.

Assessment of transmitral flow after mitral valve edge-to-edge repair using High-speed particle image velocimetry

NASA Astrophysics Data System (ADS)

Jeyhani, Morteza; Shahriari, Shahrokh; Labrosse, Michel; Kadem, Lyes

2013-11-01

Approximately 500,000 people in North America suffer from mitral valve regurgitation (MR). MR is a disorder of the heart in which the mitral valve (MV) leaflets do not close securely during systole. Edge-to-edge repair (EtER) technique can be used to surgically treat MR. This technique produces a double-orifice configuration for the MV. Under these un-physiological conditions, flow downstream of the MV forms a double jet structure that may disturb the intraventricular hemodynamics. Abnormal flow patterns following EtER are mainly characterized by high-shear stress and stagnation zones in the left ventricle (LV), which increase the potential of blood component damage. In this study, a custom-made prosthetic bicuspid MV was used to analyze the LV flow patterns after EtER by means of digital particle image velocimetry (PIV). Although the repair of a MV using EtER technique is an effective approach, this study confirms that EtER leads to changes in the LV flow field, including the generation of a double mitral jet flow and high shear stress regions.

Passive magnetic bearing for a motor-generator

DOEpatents

Post, Richard F [Walnut Creek, CA

2006-07-18

Conductive lap windings are interleaved with conventional loops in the stator of a motor-generator. The rotor provides magnetic induction lines that, when rotated, cut across the lap windings and the loops. When the rotor is laterally displaced from its equilibrium axis of rotation, its magnetic lines of induction induce a current in the interleaved lap windings. The induced current interacts with the magnetic lines of induction of the rotor in accordance with Lenz's law to generate a radial force that returns the rotor to its equilibrium axis of rotation.

Evolution of a Vietnamese Village-Part II: The Past, August 1945 to April 1964

DTIC Science & Technology

1966-04-01

Cong "Presence" in Due Lap 25 Viet Cong Approaches to Villagers 27 The Villagers ’ View of the War 30 V. THE HOA HAO COME TO DUC LAP 33 The...Indochina War when the Viet- namese fought the French for their independence. According to Ho Chi Minh, Vo Nguyen Giap, Truong Chinh , and other...the village but came from another part of Vietnam to take up the administrative duties in -20- Duc Lap. The fact that their village chief was not

Biennial Guidance Test Symposium (14th) held in Holloman Air Force Base, New Mexico on 3-5 October 1989. Volume 1

DTIC Science & Technology

1989-10-30

assembled pair is tumble lapped. Tumble lapping is a process in which Mechanically, the 1.5-inch diameter rotors a a weighted lapping element and slurry of...parameter met AGTF requirements at that site. Weighting factors were than assigned to each parameter as an indication of importance of the parameter to...AGTF. The weighted score was determined by multiplying the score by the weighting factor. The weighted scores were then totaled for each site to

Nuclear lamina genetic variants, including a truncated LAP2, in twins and siblings with nonalcoholic fatty liver disease.

PubMed

Brady, Graham F; Kwan, Raymond; Ulintz, Peter J; Nguyen, Phirum; Bassirian, Shirin; Basrur, Venkatesha; Nesvizhskii, Alexey I; Loomba, Rohit; Omary, M Bishr

2018-05-01

Nonalcoholic fatty liver disease (NAFLD) is becoming the major chronic liver disease in many countries. Its pathogenesis is multifactorial, but twin and familial studies indicate significant heritability, which is not fully explained by currently known genetic susceptibility loci. Notably, mutations in genes encoding nuclear lamina proteins, including lamins, cause lipodystrophy syndromes that include NAFLD. We hypothesized that variants in lamina-associated proteins predispose to NAFLD and used a candidate gene-sequencing approach to test for variants in 10 nuclear lamina-related genes in a cohort of 37 twin and sibling pairs: 21 individuals with and 53 without NAFLD. Twelve heterozygous sequence variants were identified in four lamina-related genes (ZMPSTE24, TMPO, SREBF1, SREBF2). The majority of NAFLD patients (>90%) had at least one variant compared to <40% of controls (P < 0.0001). When only insertions/deletions and changes in conserved residues were considered, the difference between the groups was similarly striking (>80% versus <25%; P < 0.0001). Presence of a lamina variant segregated with NAFLD independently of the PNPLA3 I148M polymorphism. Several variants were found in TMPO, which encodes the lamina-associated polypeptide-2 (LAP2) that has not been associated with liver disease. One of these, a frameshift insertion that generates truncated LAP2, abrogated lamin-LAP2 binding, caused LAP2 mislocalization, altered endogenous lamin distribution, increased lipid droplet accumulation after oleic acid treatment in transfected cells, and led to cytoplasmic association with the ubiquitin-binding protein p62/SQSTM1. Several variants in nuclear lamina-related genes were identified in a cohort of twins and siblings with NAFLD; one such variant, which results in a truncated LAP2 protein and a dramatic phenotype in cell culture, represents an association of TMPO/LAP2 variants with NAFLD and underscores the potential importance of the nuclear lamina in NAFLD. (Hepatology 2018;67:1710-1725). © 2017 by the American Association for the Study of Liver Diseases.

Face, content, and construct validity of a novel portable ergonomic simulator for basic laparoscopic skills.

PubMed

Xiao, Dongjuan; Jakimowicz, Jack J; Albayrak, Armagan; Buzink, Sonja N; Botden, Sanne M B I; Goossens, Richard H M

2014-01-01

Laparoscopic skills can be improved effectively through laparoscopic simulation. The purpose of this study was to verify the face and content validity of a new portable Ergonomic Laparoscopic Skills simulator (Ergo-Lap simulator) and assess the construct validity of the Ergo-Lap simulator in 4 basic skills tasks. Four tasks were evaluated: 2 different translocation exercises (a basic bimanual exercise and a challenging single-handed exercise), an exercise involving tissue manipulation under tension, and a needle-handling exercise. Task performance was analyzed according to speed and accuracy. The participants rated the usability and didactic value of each task and the Ergo-Lap simulator along a 5-point Likert scale. Institutional academic medical center with its affiliated general surgery residency. Forty-six participants were allotted into 2 groups: a Novice group (n = 26, <10 clinical laparoscopic procedures) and an Experienced group (n = 20, >50 clinical laparoscopic procedures). The Experienced group completed all tasks in less time than the Novice group did (p < 0.001, Mann-Whitney U test). The Experienced group also completed tasks 1, 2, and 4 with fewer errors than the Novice group did (p < 0.05). Of the Novice participants, 96% considered that the present Ergo-Lap simulator could encourage more frequent practice of laparoscopic skills. In addition, 92% would like to purchase this simulator. All of the experienced participants confirmed that the Ergo-Lap simulator was easy to use and useful for practicing basic laparoscopic skills in an ergonomic manner. Most (95%) of these respondents would recommend this simulator to other surgical trainees. This Ergo-Lap simulator with multiple tasks was rated as a useful training tool that can distinguish between various levels of laparoscopic expertise. The Ergo-Lap simulator is also an inexpensive alternative, which surgical trainees could use to update their skills in the skills laboratory, at home, or in the office. Copyright © 2014 Association of Program Directors in Surgery. Published by Elsevier Inc. All rights reserved.

Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle

PubMed Central

Das, Bhaskar; Lawrence, Mark

2017-01-01

Listeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L. monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catfish fillet attachment was investigated. To achieve this, boron-based small molecules designed to interfere with the active site of the L. monocytogenes LapB protein were developed, and their ability to prevent L. monocytogenes attachment to fish fillet was tested. Results indicated that seven out of nine different small molecules were effective in reducing the Listeria attachment to catfish fillets. Of these, three small molecules (SM3, SM5, and SM7) were highly effective in blocking Listeria attachment to catfish fillets. This study suggests an alternative strategy for reduction of L. monocytogenes contamination in fresh and frozen fish products. PMID:29253892

Lap time simulation and design optimisation of a brushed DC electric motorcycle for the Isle of Man TT Zero Challenge

NASA Astrophysics Data System (ADS)

Dal Bianco, N.; Lot, R.; Matthys, K.

2018-01-01

This works regards the design of an electric motorcycle for the annual Isle of Man TT Zero Challenge. Optimal control theory was used to perform lap time simulation and design optimisation. A bespoked model was developed, featuring 3D road topology, vehicle dynamics and electric power train, composed of a lithium battery pack, brushed DC motors and motor controller. The model runs simulations over the entire ? or ? of the Snaefell Mountain Course. The work is validated using experimental data from the BX chassis of the Brunel Racing team, which ran during the 2009 to 2015 TT Zero races. Optimal control is used to improve drive train and power train configurations. Findings demonstrate computational efficiency, good lap time prediction and design optimisation potential, achieving a 2 minutes reduction of the reference lap time through changes in final drive gear ratio, battery pack size and motor configuration.

Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa.

PubMed

Tan, A S; Worobec, E A

1993-02-01

We have isolated two alkaline phosphatases (H-AP and L-AP, for high and low molecular mass, respectively) from Pseudomonas aeruginosa PA01. These two enzymes were found to differ in mobility on sodium dodecyl sulphate polyacrylamide gels (H-AP, M(r) = 51,000 and L-AP, M(r) = 39,500), amino-terminal amino acid sequence and did not cross-react. Both enzymes were active as phosphomonoesterases while only L-AP demonstrated any phosphodiesterase activity. Both enzymes were purified from P. aeruginosa grown in phosphate limiting conditions using the same protocol and were identified in both periplasmic and extracellular locations. A low level of H-AP was produced constitutively whereas L-AP was produced only after induction by reduced phosphate concentration in the growth medium. An L-AP-like enzyme has been previously described, however, this is the first report of a second P. aeruginosa alkaline phosphatase.

Proceedings of the Midwestern Mechanics Conference (22nd) Held in Rolla, Missouri on 6-9 October 1991. Developments in Mechanics. Volume 16.

DTIC Science & Technology

1991-10-01

Mechanic;Il Engineering, University of Liverpool, 1990. II IMPACT STUDIES ON S-2 GLASS /PHENOLIC S. J. Bless B.M. Azzi N.S.Brar University of Dayton...Research Institute Dayton, OH 45469-0180 Impact failure of S-2 Glass ® composites was studied. Target resistance to penetration was determined by...There appear to be five penetration modes of glass composites - shock, double shear, single shear, cavity expansion, and tensile stretching. Two of

Distinct Functions of CCAAT Enhancer-binding Protein Isoforms in the Regulation of Manganese Superoxide Dismutase during Interleukin-1β Stimulation*

PubMed Central

Qiu, Xiaolei; Aiken, Kimberly J.; Chokas, Ann L.; Beachy, Dawn E.; Nick, Harry S.

2008-01-01

The mitochondrial antioxidant enzyme manganese superoxide dismutase (Mn-SOD) is crucial in maintaining cellular and organismal homeostasis. Mn-SOD expression is tightly regulated in a manner that synchronizes its cytoprotective functions during inflammatory challenges. Induction of Mn-SOD gene expression by the proinflammatory cytokine IL-1β is mediated through a complex intronic enhancer element. To identify and characterize the transcription factors required for Mn-SOD enhancer function, a yeast one-hybrid assay was utilized, and two CCAAT enhancer-binding protein (C/EBP) members, C/EBP β and C/EBP δ, were identified. These two transcription factors responded to IL-1β treatment with distinct expression profiles, different temporal yet inducible interactions with the endogenous Mn-SOD enhancer, and also opposite effects on Mn-SOD transcription. C/EBP β is expressed as three isoforms, LAP* (liver-activating protein), LAP, and LIP (liver-inhibitory protein). Our functional analysis demonstrated that only the full-length C/EBP β/LAP* served as a true activator for Mn-SOD, whereas LAP, LIP, and C/EBP δ functioned as potential repressors. Finally, our systematic mutagenesis of the unique N-terminal 21 amino acids further solidified the importance of LAP* in the induction of Mn-SOD and emphasized the crucial role of this isoform. Our data demonstrating the physiological relevance of the N-terminal peptide also provide a rationale for revisiting the role of LAP* in the regulation of other genes and in pathways such as lipogenesis and development. PMID:18559338

The Characteristics of Fatigue Damage in the Fuselage Riveted Lap Splice Joint

NASA Technical Reports Server (NTRS)

Piascik, Robert S.; Willard, Scott A.

1997-01-01

An extensive data base has been developed to form the physical basis for new analytical methodology to predict the onset of widespread fatigue damage in the fuselage lap splice joint. The results of detailed destructive examinations have been cataloged to describe the physical nature of MSD in the lap splice joint. ne catalog includes a detailed description, e.g., crack initiation, growth rates, size, location, and fracture morphology, of fatigue damage in the fuselage lap splice joint structure. Detailed examinations were conducted on a lap splice joint panel removed from a full scale fuselage test article after completing a 60,000 cycle pressure test. The panel contained a four bay region that exhibited visible outer skin cracks and regions of crack link-up along the upper rivet row. Destructive examinations revealed undetected fatigue damage in the outer skin, inner skin, and tear strap regions. Outer skin fatigue cracks were found to initiate by fretting damage along the faying surface. The cracks grew along the faying surface to a length equivalent to two to three skin thicknesses before penetrating the outboard surface of the outer skin. Analysis of fracture surface marker bands produced during full scale testing revealed that all upper rivet row fatigue cracks contained in a dim bay region grow at similar rates; this important result suggests that fracture mechanics based methods can be used to predict the growth of outer skin fatigue cracks in lap splice structure. Results are presented showing the affects of MSD and out-of-plane pressure loads on outer skin crack link-up.

Feasibility, safety, and preliminary efficacy of Low Amplitude Seizure Therapy (LAP-ST): A proof of concept clinical trial in man.

PubMed

Youssef, Nagy A; Sidhom, Emad

2017-11-01

Current pulse amplitude used in clinical ECT may be higher than needed. Reducing pulse amplitude may improve focality of the electric field and thus cognitive adverse effects. Here we examine the feasibility, safety, and whether Low Pulse Amplitude Seizure Therapy (LAP-ST, 0.5-0.6A) minimizes cognitive adverse effects while retaining efficacy. Patients with treatment-resistant primary mood (depressive episodes) or psychotic disorders who were clinically indicated to undergo ECT were offered to be enrolled in an open-label study. The study consisted of a full acute course of LAP-ST under standard anesthesia and muscle relaxation. The primary outcome was feasibility of seizure induction. Clinical outcome measures were: time to reorientation (TRO), Mini Mental State Examination, Montgomery Aberg Depression Scale, and Brief Psychiatric Rating Scale, and Clinical Global Impression Scale. Twenty-two patients consented for enrollment in the study. LAP-ST was feasible, and all patients had seizures in the first session. Participants had a quick orientation with median TRO of 4.5min. Treatment was efficacious for both depressive and psychotic symptoms. Relatively small sample size, non-blinded, and no randomization was performed in this initial proof of concept study. This first human preliminary data of a full course of focal LAP-ST demonstrates that seizure induction is feasible. These results, although preliminary, suggest that the LAP-ST compared to the standard ECT techniques may result in less cognitive side effects, but comparable efficacy. Larger studies are needed to replicate these findings. Copyright © 2017 Elsevier B.V. All rights reserved.

Long-term, repeated dose in vitro neurotoxicity of the glutamate receptor antagonist L-AP3, demonstrated in rat hippocampal slice cultures by using continuous propidium iodide incubation.

PubMed

Kristensen, Bjarne W; Blaabjerg, Morten; Noraberg, Jens; Zimmer, Jens

2007-05-01

Most in vitro models are only used to assess short-term effects of test compounds. However, as demonstrated here, hippocampal slice cultures can be used for long-term studies. The test compound used was the metabotropic glutamate receptor antagonist, L(+)-2-amino-3-phosphonopropionic acid (L-AP3), which is known to be toxic in vivo after subchronic, but not acute, administration. Degenerative effects were monitored by measuring the cellular uptake of propidium iodide (PI; continuously present in the medium) and lactate dehydrogenase (LDH) leakage, and by using a panel of histological stains. Hippocampal slices, derived from 2-3 day old rats and grown for 3 weeks, were subsequently exposed for the next 3 weeks to 0, 10 or 100microM L-AP3, with PI (2microM) in the culture medium. Exposure to 100microM L-AP3 induced severe toxicity after 4-6 days, shown by massive PI uptake, LDH leakage, changes in MAP2 and GFAP immunostaining, and in Nissl and Timm staining. In contrast, 10microM L-AP3 did not induce detectable neuronal degeneration. Treatment with the NMDA receptor antagonist, MK-801, or the AMPA/KA receptor antagonist NBQX, together with 100microM L-AP3, reduced neurodegeneration down to close to control values. It is concluded that continuous incubation of hippocampal slice cultures with PI is technically feasible for use in studies of inducible neuronal degeneration over time.

Lap-Dissolve Slides

ERIC Educational Resources Information Center

Fine, Leonard W.; And Others

1977-01-01

Discusses the use of lap-dissolve projection to give students pre-laboratory instruction on an upcoming experiment. In this technique, two slide projectors are operated alternately so that one visual image fades away while the next appears on the same screen area. (MLH)

INTERIOR OF WEST SPAN LOOKING WEST (SHADOW OF VERTICAL LAPS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

INTERIOR OF WEST SPAN LOOKING WEST (SHADOW OF VERTICAL LAPS PLACED ON ZONE III; ASPHALT ZONE IX) - Honey Run Bridge, Spanning Butte Creek, bypassed section of Honey Run Road (originally Carr Hill Road), Paradise, Butte County, CA

Impact injuries in pregnancy. I, Experimental studies.

DOT National Transportation Integrated Search

1968-03-01

Eleven pregnant baboons were subjected to experimental impacts. Variables included 20-, 33-, and 40-G levels of decelerative forces; forward and rearward facing seats; lap belt and lap belt plus shoulder harness restraint; and seat pans tilted 20 deg...

77 FR 28240 - Airworthiness Directives; The Boeing Company Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-14

... multiple site damage cracks in the radial web lap and tear strap splices of the aft pressure bulkhead at... multiple site damage cracks in the radial web lap and tear strap splices of the aft pressure bulkhead at...

Does training novices to criteria and does rapid acquisition of skills on laparoscopic simulators have predictive validity or are we just playing video games?

PubMed

Hogle, Nancy J; Widmann, Warren D; Ude, Aku O; Hardy, Mark A; Fowler, Dennis L

2008-01-01

To determine whether LapSim training (version 3.0; Surgical Science Ltd, Göteborg, Sweden) to criteria for novice PGY1 surgical residents had predictive validity for improvement in the performance of laparoscopic cholecystectomy. In all, 21 PGY1 residents performed laparoscopic cholecystectomies in pigs after minimal training; their performance was evaluated by skilled laparoscopic surgeons using the validated tool GOALS (global operative assessment of laparoscopic operative skills: depth perception, bimanual dexterity, efficiency, tissue handling, and overall competence). From the group, 10 residents trained to competency on the LapSim Basic Skills Programs (camera navigation, instrument navigation, coordination, grasping, lifting and grasping, cutting, and clip applying). All 21 PGY1 residents again performed laparoscopic cholecystectomies on pigs; their performance was again evaluated by skilled laparoscopic surgeons using GOALS. Additionally, we studied the rate of learning to determine whether the slow or fast learners on the LapSim performed equivalently when performing actual cholecystectomies in pigs. Finally, 6 categorical residents were tracked, and their clinical performance on all of the laparoscopic cholecystectomies in which they were "surgeon, junior" was prospectively evaluated using the GOALS criteria. We found a statistical improvement of depth perception in the operative performance of cholecystectomies in pigs in the group trained on the LapSim. In the other 4 domains, a trend toward improvement was observed. No correlation between being a fast learner and the ultimate skill was demonstrated in the clinical performance of laparoscopic cholecystectomies. We did find that the fast learners on LapSim all were past or current video game players ("gamers"); however, that background did not translate into better clinical performance. Using current criteria, we doubt that the time and effort spent training novice PGY1 Surgical Residents on the basic LapSim training programs is justified, as such training to competence lacks predictive validity in most domains of the GOALS program. We are investigating 2 other approaches: more difficult training exercises using the LapSim system and an entirely different approach using haptic technology (ProMis; Haptica Ltd., Ireland), which uses real instruments, with training on realistic 3-dimensional models with real rather than simulated cutting, sewing, and dissection. Although experienced video gamers achieve competency faster than nongamers on LapSim programs, that skill set does not translate into improved clinical performance.

Highly elastic polymer solutions under shear: Polymer migration, viscoelastic instabilities, and anomalous rheology

DOE Office of Scientific and Technical Information (OSTI.GOV)

MacDonald, M.J.; Muller, S.J.

1996-12-31

The use of highly elastic polymer solutions has been remarkably successful in elucidating the behavior of polymeric materials under flowing conditions. Here, we present the results of an extensive experimental study into the shear behavior of an athermal, dilute, binary polymer solution that is believed to be free of many of these effects. Under extended shearing, we observe the migration of polymer species: after shearing for several hundred hours, concentrations that are more than double the initial uniform value can be achieved. Although the solutions are well-described by dumbbell models in shear flows on short-time scales, theoretical predictions substantially underestimatemore » the rate of migration. Flow visualization and rheometric experiments suggest that the origin of this discrepancy could be the anomalous long-time rheology of these solutions. While these fluids display the well-known elastic instability in cone and plate flow above a critical Deborah number, extended shearing reveals that the toroidal secondary flow is eventually replaced by a purely azimuthal shearing flow. In addition, when sheared below the critical condition for the instability, the solutions exhibit a slow but reversible decay in normal stresses. The shear-induced migration of polymer species has been predicted by numerous theoretical studies. However, observations on the highly elastic polymer solutions that are most likely to show polymer migration, are complicated by a number of different physical processes that occur as a result of shearing. These phenomena, which include shear-induced phase separation, elastically-induced hydrodynamic instabilities, mixed solvent effects, shear-induced aggregation, and anomalous transient shear and normal stress behavior are often observed at times earlier than and at shear rates less than those where migration is predicted to occur; hence, the experimental detection of polymer migration has been thwarted by these other physical processes.« less

Evaluation of high temperature structural adhesives for extended service. [supersonic cruise aircraft research

NASA Technical Reports Server (NTRS)

Hill, S. G.

1981-01-01

Eight different Ti-6Al-4V surface treatments were investigated for each of 10 candidate resins. Primers (two for each resin) were studied for appropriate cure and thickness and initial evaluation of bond joints began using various combinations of the adhesive resins and surface treatments. Surface failure areas of bonded titanium coupons were analyzed by electron microscopy and surface chemical analysis techniques. Results of surface characterization and failure analysis are described for lap shear bond joints occurring with adhesive systems consisting of: (1) LARC-13 adhesive, Pasa jell surface treatment; (2) LARC-13 adhesive, 10 volt CAA treatment; (3) PPQ adhesive, 10 volt CAA treatment; and (4) PPQ adhesive, 5 volt CAA treatment. The failure analysis concentrated on the 10,000 hr 505K (450 F) exposed specimens which exhibited adhesive failure. Environmental exposure data being generated on the PPQ-10 volt CAA and the LARC-TPI-10 volt CAA adhesive systems is included.

Screening of high temperature adhesives for large area bonding

NASA Technical Reports Server (NTRS)

Stenersen, A. A.; Wykes, D. H.

1980-01-01

High temperature-resistant adhesive systems were screened for processability, mechanical and physical properties, operational capability at 589 K (600 F), and the ability to produce large area bonds of high quality in fabricating Space Shuttle components. The adhesives consisted primarily of polyimide systems, including FM34B-18, NR-150B2 (DuPont), PMR-15, LARC-13, LARC-160, Thermid 600, and AI-1130L (AMOCA). The processing studies included preparation of polyimide resins, fabrication of film adhesives, development of lay-up and cure procedures, fabrication of honeycomb sandwich panels, and fabrication of mid-plane bonded panels in joints up to 30.5 cm (12 in.) wide. The screening program included tests for tack and drape properties, reticulation and filleting characteristics, ability to produce void-free or low porosity bonds in mid-plane bonded panels, out-time stability, lap shear strength, climbing drum peel strength, and glass transition temperature (Tg). This paper describes the processing methods developed and the test results.

LARC-IA: A flexible backbone polyimide

NASA Technical Reports Server (NTRS)

Progar, Donald J.; Stclair, Terry L.

1990-01-01

A new linear, aromatic, thermoplastic polyimide, prepared from oxydiphthalic anhydride (ODPA) and 3,4'-oxydianiline (ODA) in diglyme and identified as LARC-IA, was synthesized and evaluated. The monomers are relatively inexpensive and physiologically safe. Molecular weight was controlled by use of a monofunctional anhydride, phthalic anhydride (PA), in order to promote controlled flow and wetting properties. The polymer is considered a safe alternative to commercially available LARC-TPI which is prepared with an expensive diamine of uncertain carcinogenicity. The evaluation was based primarily on the polymer's adhesive properties as determined by thermal and water boil exposure of lap shear specimens. Strengths were determined at room temperature, 177, 204 and 232 C before and after exposure to determine the adhesive system's durability to adverse environments over a period of time. Other properties (FWT, G(1c), film and composite properties) were examined which were determined to be typical of a high temperature polyimide. Results of the study show a favorable comparison to LARC-TPI, a commercially available polyimide.

Residual Silicone Detection. [external tank and solid rocket booster surfaces

NASA Technical Reports Server (NTRS)

Smith, T.

1980-01-01

Both photoelectron emission and ellipsometry proved successful in detecting silicone contamination on unpainted and epoxy painted metal surfaces such as those of the external tank and the solid rocket booster. Great success was achieved using photoelectron emission (PEE). Panels were deliberately contaminated to controlled levels and then mapped with PEE to reveal the areas and levels that were contaminated. The panels were then tested with regard to adhesive properties. Tapes were bonded over the contaminated and uncontaminated regions and the peel force was measured, or the contaminated panels were bonded (with CPR 483 foam) to uncontaminated panels and made into lap shear specimens. Other panels were bonded and made into wedge specimens for hydrothermal stress endurance tests. Strong adhesion resulted if the PEE signal fell within an acceptance window, but was poor outside the acceptance window. A prototype instrument is being prepared which can automatically be scanned over the external liquid hydrogen tank and identify those regions that are contaminated and will cause bond degradation.

Influence of Thin-Film Adhesives in Pullout Tests Between Nickel-Titanium Shape Memory Alloy and Carbon Fiber-Reinforced Polymer Matrix Composites

NASA Technical Reports Server (NTRS)

Quade, Derek J.; Jana, Sadhan; McCorkle, Linda S.

2018-01-01

Strips of nickel-titanium (NiTi) shape memory alloy (SMA) and carbon fiber-reinforced polymer matrix composite (PMC) were bonded together using multiple thin film adhesives and their mechanical strengths were evaluated under pullout test configuration. Tensile and lap shear tests were conducted to confirm the deformation of SMAs at room temperature and to evaluate the adhesive strength between the NiTi strips and the PMC. Optical and scanning electron microscopy techniques were used to examine the interfacial bonding after failure. Simple equations on composite tensile elongation were used to fit the experimental data on tensile properties. ABAQUS models were generated to show the effects of enhanced bond strength and the distribution of stress in SMA and PMC. The results revealed that the addition of thin film adhesives increased the average adhesive strength between SMA and PMC while halting the room temperature shape memory effect within the pullout specimen.

Poly(imide-siloxane) segmented copolymer structural adhesives prepared by bulk and solution thermal imidization

NASA Technical Reports Server (NTRS)

Bott, R. H.; Summers, J. D.; Arnold, C. A.; Blankenship, C. P., Jr.; Taylor, L. T.

1988-01-01

The improved properties that have been demonstrated through thermal solution imidization in the case of polyimides and poly(imide-siloxane) segmented copolymers suggests significant potential for application of these new materials. Specifically, the enhancement in solubility, moisture reduction, and processability observed through this solution technique is quite dramatic. Previous work has shown that the presence of low amounts of siloxane does not detract significantly from the lap shear strength of these materials to titanium in the case of bulk thermal imidization synthesis. In addition, the siloxane incorporation results in the added advantage of resistance to hot, wet environments. This added durability is presumably due to the hydrophobic siloxane segments preventing the uptake of water at the critical interphase between the adhesive and the adherend. This paper discusses the extension of this work to the solution imidization synthesis technique recently developed in our laboratory. Results dealing with the absolute bond strengths as well as durability and failure surface analysis will be presented.