Develop to Term Rat Oocytes Injected with Heat-Dried Sperm Heads

PubMed Central

Lee, Kyung-Bon; Park, Ki-Eun; Kwon, In-Kiu; Tripurani, Swamy K.; Kim, Keun Jung; Lee, Ji Hye; Niwa, Koji; Kim, Min Kyu

2013-01-01

This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation and cleavage of oocytes were worse in oocytes injected with spermatozoa heat-dried and stored for 1 week than unheated, fresh spermatozoa, but in heat-dried spermatozoa, there were no differences in these abilities of oocytes between the samples stored in nitrogen gas and in air. The oocytes injected with heat-dried spermatozoa stored for 1 week could develop to the morula and blastocyst stages without difference between the samples stored in nitrogen gas and in air after artificial stimulation. Cleavage of oocytes and development of cleaved embryos were higher when heat-dried spermatozoa were stored for 3 and 6 months in nitrogen gas than in air. However, the ability of injected oocytes to develop to the morula and blastocyst stages was not inhibited even when heat-dried spermatozoa stored in both atmosphere conditions for as long as 6 months were used. When 2-cell embryos derived from oocytes injected with heads from spermatozoa heat-dried and stored for 1 week and 1 month were transferred, each 1 of 4 recipients was conceived, and the conceived recipients delivered 1 live young each. These results demonstrate that rat oocytes can be fertilized with heat-dried spermatozoa and that the fertilized oocytes can develop to term. PMID:24223784

Study on cyclic injection gas override in condensate gas reservoir

NASA Astrophysics Data System (ADS)

Sun, Yan; Zhu, Weiyao; Xia, Jing; Li, Baozhu

2018-02-01

Cyclic injection gas override in condensate gas reservoirs for the large density difference between injection gas and condensate gas has been studied, but no relevant mathematical models have been built. In this paper, a mathematical model of cyclic injection gas override in condensate gas reservoir is established, considering density difference between the injected gas and the remaining condensate gas in the formation. The vertical flow ratio and override degree are used to reflect the override law of injected dry gas. Combined with the actual data of Tarim gas condensate reservoir, the parameters of injected dry gas override are calculated and analysed. The results show that the radial pressure rises or falls rapidly and the pressure gradient varies greatly in the near wells. The radial pressure varies slowly and the pressure gradient changes little in the reservoir which is within a certain distance from the wells. In the near injection well, the injected dry gas mainly migrates along the radial direction, and the vertical migration is relatively not obvious. With the distance from the injection well, the vertical flow ratio and override degree of injected dry gas increases, and the vertical flow ratio reaches the maximum in the middle of the injection well and the production well.

Hot air injection for removal of dense, non-aqueous-phase liquid contaminants from low-permeability soils

DOE Office of Scientific and Technical Information (OSTI.GOV)

Payne, F.C.

1996-08-01

The performance of soil vapor extraction systems for the recovery of volatile and semi-volatile organic compounds is potentially enhanced by the injection of heated air to increase soil temperatures. The soil temperature increase is expected to improve soil vapor extraction (SVE) performance by increasing target compound vapor pressures and by increasing soil permeability through drying. The vapor pressure increase due to temperature rise relieves the vapor pressure limit on the feasibility of soil vapor extraction. However, the system still requires an air flow through the soil system to deliver heat and to recover mobilized contaminants. Although the soil permeability canmore » be increased through drying, very low permeability soils and low permeability soils adjacent to high permeability air flow pathways will be treated slowly, if at all. AR thermal enhancement methods face this limitation. Heated air injection offers advantages relative to other thermal techniques, including low capital and operation costs. Heated air injection is at a disadvantage relative to other thermal techniques due to the low heat capacity of air. To be effective, heated air injection requires that higher air flows be established than for steam injection or radio frequency heating. Heated air injection is not economically feasible for the stratified soil system developed as a standard test for this document. This is due to the inability to restrict heated air flow to the clay stratum when a low-resistance air flow pathway is available in the adjoining sand. However, the technology should be especially attractive, both technically and economically, for low-volatile contaminant recovery from relatively homogeneous soil formations. 16 refs., 2 tabs.« less

Systematic review of randomized controlled trials in the treatment of dry eye disease in Sjogren syndrome.

PubMed

Shih, Kendrick Co; Lun, Christie Nicole; Jhanji, Vishal; Thong, Bernard Yu-Hor; Tong, Louis

2017-01-01

Primary Sjögren's syndrome is an autoimmune disease characterized by dry eye and dry mouth. We systematically reviewed all the randomized controlled clinical trials published in the last 15 years that included ocular outcomes. We found 22 trials involving 9 topical, 10 oral, 2 intravenous and 1 subcutaneous modalities of treatment. Fluoromethalone eye drops over 8 weeks were more effective than topical cyclosporine in the treatment of dry eye symptoms and signs; similarly, indomethacin eye drops over 1 month were more efficacious than diclofenac eye drops. Oral pilocarpine 5 mg twice daily over 3 months was superior to use of lubricants or punctal plugs for treating dry eye, but 5% of participants had gastrointestinal adverse effects from pilocarpine, though none discontinued treatment. In contrast, etanercept, a TNF-alpha blocking antibody, administered as subcutaneous injections twice weekly, did not improve dry eye significantly compared to placebo injections. In conclusion, topical corticosteroids have been shown to be effective in dry eye associated with Sjögren's syndrome. As some topical non-steroidal anti-inflammatory drugs may be more effective than others, these should be further evaluated. Systemic secretagogues like pilocarpine have a role in Sjögren's syndrome but the adverse effects may limit their clinical use. It is disappointing that systemic cytokine therapy did not produce encouraging ocular outcomes but participants should have assessment of cytokine levels in such trials, as those with higher baseline cytokine levels may respond better. (229 words).

[Optimization of vacuum belt drying process of Gardeniae Fructus in Reduning injection by Box-Behnken design-response surface methodology].

PubMed

Huang, Dao-sheng; Shi, Wei; Han, Lei; Sun, Ke; Chen, Guang-bo; Wu Jian-xiong; Xu, Gui-hong; Bi, Yu-an; Wang, Zhen-zhong; Xiao, Wei

2015-06-01

To optimize the belt drying process conditions optimization of Gardeniae Fructus extract from Reduning injection by Box-Behnken design-response surface methodology, on the basis of single factor experiment, a three-factor and three-level Box-Behnken experimental design was employed to optimize the drying technology of Gardeniae Fructus extract from Reduning injection. With drying temperature, drying time, feeding speed as independent variables and the content of geniposide as dependent variable, the experimental data were fitted to a second order polynomial equation, establishing the mathematical relationship between the content of geniposide and respective variables. With the experimental data analyzed by Design-Expert 8. 0. 6, the optimal drying parameter was as follows: the drying temperature was 98.5 degrees C , the drying time was 89 min, the feeding speed was 99.8 r x min(-1). Three verification experiments were taked under this technology and the measured average content of geniposide was 564. 108 mg x g(-1), which was close to the model prediction: 563. 307 mg x g(-1). According to the verification test, the Gardeniae Fructus belt drying process is steady and feasible. So single factor experiments combined with response surface method (RSM) could be used to optimize the drying technology of Reduning injection Gardenia extract.

The dependence of permeability on effective stress from flow tests at hot dry rock reservoirs at Rosemanowes (Cornwall) and Fenton Hill (New Mexico)

USGS Publications Warehouse

Nathenson, M.

1999-01-01

Effective stress is the primary control on permeability and thus on flow and water loss for two-well hot dry rock systems involving injection and production that have been tested to date. Theoretical relations are derived for the flow between an injector and producer, including the dependence of permeability on effective stress. Four relations for permeability as a function of effective stress are used to match field data for the hot dry rock systems at Rosemanowes, Cornwall, and Fenton Hill, New Mexico. The flow and water loss behavior of these systems are well explained by the influence of effective stress on permeability. All four relations for permeability as a function of effective stress are successful in matching the field data, but some have difficulty in determining unique values for elastic and hydrologic parameters.Effective stress is the primary control on permeability and thus on flow and water loss for two-well hot dry rock systems involving injection and production that have been tested to date. Theoretical relations are derived for the flow between an injector and producer, including the dependence of permeability on effective stress. Four relations for permeability as a function of effective stress are used to match field data for the hot dry rock systems at Rosemanowes, Cornwall, and Fenton Hill, New Mexico. The flow and water loss behavior of these systems are well explained by the influence of effective stress on permeability. All four relations for permeability as a function of effective stress are successful in matching the field data, but some have difficulty in determining unique values for elastic and hydrologic parameters.

Magnetized Target Fusion - Field Reversed Configuration Formation and Injection (MTF-FRC)

DTIC Science & Technology

2009-11-06

from accidental breakage and personnel from injury in that event. The pumps for the vacuum system included a Varian dry scroll pump that was...a dry scroll (oil-free) mechanical pump could be used, as mTorr pressures would be sufficient for the vacuum switch voltage hold-off and operation...56 FIGURE 46. ROUGHING PUMP AND VACUUM -GAUGE CONTROLLERS BENEATH THETA COIL CABLE HEADER

77 FR 3712 - Approval and Promulgation of Air Quality Implementation Plans; Ohio; Regional Haze

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-25

... equal incremental change in visibility perceived by the human eye. Most people can detect a change in... desulfurization (FGD), semi-dry FGD, and over-fire air (OFA) with a sorbent injection system (SIS). Ohio and MPRO... on up to 7 days a year. Ohio selected semi-dry FGD as the BART SO 2 control, which is expected to...

Central Heating Plant Coal Use Handbook. Volume 1: Technical Reference.

DTIC Science & Technology

1996-11-01

variation of a dry desulfurization system simply uses dry calcium hydroxide that is injected into the flue gas stream before entry to a fabric filter...97/14, Voll 173 competitive capital and operating costs compared with conventional technology using flue gas desulfurization reduced NOx emissions...ferric iron in slag, expressed as a percentage of the total iron calculated as ferric iron FGD: Flue gas desulfurization filter: A device for

EVALUATION OF FGD DRY INJECTION SORBENTS AND ADDITIVES - VOLUME 2 - PILOT PLANT EVALUATION OF HIGH REACTIVITY SORBENTS

EPA Science Inventory

The report describes a mini-pilot test program to investigate potential new sorbents and processes for dry SO2 removal. Initial tests showed that the 85 cu m/h pilot plant could be used successfully to evaluate both spray dryer and dry injection processes using traditional calciu...

Comparison of the therapeutic effects of ultrasound-guided platelet-rich plasma injection and dry needling in rotator cuff disease: a randomized controlled trial.

PubMed

Rha, Dong-wook; Park, Gi-Young; Kim, Yong-Kyun; Kim, Min Tae; Lee, Sang Chul

2013-02-01

To compare the effects of platelet-rich plasma injection with those of dry needling on shoulder pain and function in patients with rotator cuff disease. A single-centre, prospective, randomized, double-blinded, controlled study. University rehabilitation hospital. Thirty-nine patients with a supraspinatus tendon lesion (tendinosis or a partial tear less than 1.0 cm, but not a complete tear) who met the inclusion criteria recruited between June 2010 and February 2011. Two dry needling procedures in the control group and two platelet-rich plasma injections in the experimental group were applied to the affected shoulder at four-week intervals using ultrasound guidance. The Shoulder Pain and Disability Index, passive range of motion of the shoulder, a physician global rating scale at the six-month follow-up, adverse effects monitoring and an ultrasound measurement were used as outcome measures. The clinical effect of the platelet-rich plasma injection was superior to the dry needling from six weeks to six months after initial injection (P < 0.05). At six months the mean Shoulder Pain and Disability Index was 17.7 ± 3.7 in the platelet-rich plasma group versus 29.5 ± 3.8 in the dry needling group (P < 0.05). No severe adverse effects were observed in either group. Autologous platelet-rich plasma injections lead to a progressive reduction in the pain and disability when compared to dry needling. This benefit is certainly still present at six months after treatment. These findings suggest that treatment with platelet-rich plasma injections is safe and useful for rotator cuff disease.

A freeze-dried injectable form of ibuprofen: development and optimisation using response surface methodology.

PubMed

Kagkadis, K A; Rekkas, D M; Dallas, P P; Choulis, N H

1996-01-01

In this study a complex of Ibuprofen and b-Hydroxypropylcyclodextrin was prepared employing a freeze drying method. The production parameters and the final specifications of this product were optimized by using response surface methodology. The results show that the freeze dried complex meets the requirements for solubility to be considered as a possible injectable form.

Giving an IM (intramuscular) injection

MedlinePlus

... Wash your hands well with soap and water. Dry them. Carefully find the spot where you will give the injection. Clean the skin at that spot with an alcohol wipe. Let it dry. Take the cap off the needle. Hold the ...

Choosing Wisely When It Comes to Eye Care: Antibiotics for Eye Injections

MedlinePlus

... Antibiotics for eye injections; and Punctal plugs for dry eye . This is the fourth in a series of ... why patients and their ophthalmologists should discuss treating dry eye with punctal plugs only after other treatment options ...

Injection molding ceramics to high green densities

NASA Technical Reports Server (NTRS)

Mangels, J. A.; Williams, R. M.

1983-01-01

The injection molding behavior of a concentrated suspension of Si powder in wax was studied. It was found that the injection molding behavior was a function of the processing techniques used to generate the powder. Dry ball-milled powders had the best molding behavior, while air classified and impact-milled powders demonstrated poorer injection moldability. The relative viscosity of these molding batches was studied as a function of powder properties: distribution shape, surface area, packing density, and particle morphology. The experimental behavior, in all cases, followed existing theories. The relative viscosity of an injection molding composition composed of dry ball-milled powders could be expressed using Farris' relation.

CALCINATION AND SINTERING OF SORBENTS DURING BOILER INJECTION FOR DRY SULFUR DIOXIDE CONTROL

EPA Science Inventory

The paper discusses the calcination and sintering of sorbents during boiler injection for dry sulfur dioxide (S02) control, with emphasis on calcium hydroxide--Ca(OH)2--because of its superior reactivity with S02 and its wide commercial availability. Calcination and sintering are...

Pharmaceutical patent applications in freeze-drying.

PubMed

Ekenlebie, Edmond; Einfalt, Tomaž; Karytinos, Arianna Irò; Ingham, Andrew

2016-09-01

Injectable products are often the formulation of choice for new therapeutics; however, formulation in liquids often enhances degradation through hydrolysis. Thus, freeze-drying (lyophilization) is regularly used in pharmaceutical manufacture to reduce water activity. Here we examine its contribution to 'state of the art' and look at its future potential uses. A comprehensive search of patent databases was conducted to characterize the international patent landscape and trends in the use of freeze-drying. A total of 914 disclosures related to freeze-drying, lyophilization or drying of solid systems in pressures and temperatures equivalent to those of freeze-drying were considered over the period of 1992-2014. Current applications of sublimation technology were contrasted across two periods those with patents due to expire (1992-1993) and those currently filed. The number of freeze-drying technology patents has stabilized after initial activity across the biotechnology sector in 2011 and 2012. Alongside an increasing trend for patent submissions, freeze-drying submissions have slowed since 2002 and is indicative of a level of maturity.

[Investigation on Spray Drying Technology of Auricularia auricular Extract].

PubMed

Zhou, Rong; Chen, Hui; Xie, Yuan; Chen, Peng; Wang, Luo-lin

2015-07-01

To investigate the feasibility of spray drying technology of Auricularia auricular extract and its optimum process. On the basis of single factor test, with the yield of dry extract and the content of polysaccharide as indexes, orthogonal test method was used to optimize the spray drying technology on the inlet air temperature, injection speed and crude drug content. Using ultraviolet spectrophotometry, thin layer chromatography(TLC) and pharmacodynamics as indicators, extracts prepared by traditional alcohol precipitation drying process and spray drying process were compared. Compared with the traditional preparation method, the extract prepared by spray drying had little differences from the polysaccharide content, TLC and the function of reducing TG and TC, and its optimum technology condition were as follows: The inlet air temperature was 180 °C, injection speed was 10 ml/min and crude drugs content was 0. 4 g/mL. Auricularia auricular extract by spray drying technology is stable and feasible with high economic benefit.

Fabrication of sinterable silicon nitride by injection molding

NASA Technical Reports Server (NTRS)

Quackenbush, C. L.; French, K.; Neil, J. T.

1982-01-01

Transformation of structural ceramics from the laboratory to production requires development of near net shape fabrication techniques which minimize finish grinding. One potential technique for producing large quantities of complex-shaped parts at a low cost, and microstructure of sintered silicon nitride fabricated by injection molding is discussed and compared to data generated from isostatically dry-pressed material. Binder selection methodology, compounding of ceramic and binder components, injection molding techniques, and problems in binder removal are discussed. Strength, oxidation resistance, and microstructure of sintered silicon nitride fabricated by injection molding is discussed and compared to data generated from isostatically dry-pressed material.

Research on terahertz properties of rat brain tissue sections during dehydration

NASA Astrophysics Data System (ADS)

Cui, Gangqiang; Liang, Jianfeng; Zhao, Hongwei; Zhao, Xianghui; Chang, Chao

2018-01-01

Biological tissue sections are always kept in a system purged with dry nitrogen for the measurement of terahertz spectrum. However, the injected nitrogen will cause dehydration of tissue sections, which will affect the accuracy of spectrum measurement. In this paper, terahertz time-domain spectrometer is used to measure the terahertz spectra of rat brain tissue sections during dehydration. The changes of terahertz properties, including terahertz transmittance, refractive index and extinction coefficient during dehydration are also analyzed. The amplitudes of terahertz time-domain spectra increase gradually during the dehydration process. Besides, the terahertz properties show obvious changes during the dehydration process. All the results indicate that the injected dry nitrogen has a significant effect on the terahertz spectra and properties of tissue sections. This study contributes to further research and application of terahertz technology in biomedical field.

Mechanical and thermal properties of high density polyethylene – dried distillers grains with solubles composites

USDA-ARS?s Scientific Manuscript database

Dried Distillers Grain with Solubles (DDGS) is evaluated as a bio-based fiber reinforcement. Injection molded composites of high density polyethylene (HDPE), 25% by weight of DDGS, and either 5% of 0% by weight of maleated polyethylene (MAPE) were produced by twin screw compounding and injection mo...

Comparison of dry needling and steroid injection in the treatment of plantar fasciitis: a single-blind randomized clinical trial.

PubMed

Rastegar, Shirvan; Baradaran Mahdavi, Sadegh; Hoseinzadeh, Babak; Badiei, Sajad

2018-01-01

Plantar fasciitis is a common cause of heel pain. Considering different interventions which are applied for patients with plantar fasciitis, dry needling is proposed as a new modality of treatment recently. The aim of this study is to evaluate the effectiveness of dry needling versus steroid injection for plantar fasciitis. Sixty-six patients were recruited to this single-blind clinical trial study. Participants were randomly allocated to receive 1 ml (40 mg) of Depo-Medrol (methylprednisolone acetate) or dry needling. They were followed up for 12 months and monitored for total perception of pain using the visual analogue scale (VAS), with data obtained in baseline and at three weeks, six weeks, three months, six months and one year after treatment. Mean VAS score before treatment was 6.96 ± 0.87 for the steroid group and 6.41 ± 0.83 for the dry-needling group (P value = 0.54). Steroid injection reduced VAS scores rapidly until three weeks after treatment compared with dry needling (0.32 ± 0.71 and 3.47 ± 1.32, respectively; P value < 0.001). However, patients who were underwent dry needling reported lower VAS scores at the end of follow-up compared with the steroid group (0.69 ± 0.93 and 2.09 ± 1.58, respectively; P value = 0.004). Over the long term, 82.3% and 17.6% of changes in pain were contributed to time since treatment and treatment method, respectively (P values < 0.001). Steroid injection can palliate plantar heel pain rapidly but dry needling can provide more satisfactory results for patients with plantar fasciitis in the long term.

Structural integrity and developmental potential of spermatozoa following microwave-assisted drying in the domestic cat model.

PubMed

Patrick, Jennifer L; Elliott, Gloria D; Comizzoli, Pierre

2017-11-01

Characterizing the resilience of mammalian cells to non-physiological conditions is necessary to develop preservation and long-term storage strategies at low or ambient temperatures. Using the domestic cat model, the objective of the study was to characterize structural integrity (morphology and DNA damage) as well as functional properties (sperm aster formation and embryo formation after sperm injection) of spermatozoa after microwave-assisted drying to a moisture content compatible with storage in a glassy state at supra-zero temperatures. In Experiment 1, cat epididymal spermatozoa were porated with hemolysin and dried (using a commercial microwave oven set to 20% power) in the presence of trehalose for up to 50 min in a low humidity environment (11%) before measuring moisture content and sample temperature. In Experiment 2, morphology and DNA integrity were evaluated in sperm dried for up to 30 min (using the same method as above) versus fresh spermatozoa. In Experiment 3, the functionality of sperm dried for 30 min versus fresh sperm cells was evaluated after injection into oocytes based on sperm aster formation (5 h post-injection) and embryo development in vitro over 7 days. Moisture contents compatible with dry state storage were reached after 30 min of microwave-assisted drying. After rehydration, sperm morphology was not affected and the percentages of cells with damaged DNA (∼6.5%) was similar to the fresh controls. Sperm aster diameters appeared to be generally smaller for dried-rehydrated cells compared to the fresh controls. This observation was consistent with a lower proportion of blastocyst formation after injection with dried spermatozoa (6.5%) compared to fresh spermatozoa (15%). However, the blastocyst quality based on the total blastomere number was not affected by the sperm treatment. This is the first and encouraging report in any species so far demonstrating that spermatozoa can be dried using microwaves without causing irreversible damage to the cellular structure and function. Published by Elsevier Inc.

Topical steroid and non-steroidal anti-inflammatory drugs inhibit inflammatory cytokine expression on the ocular surface in the botulinum toxin B-induced murine dry eye model.

PubMed

Zhu, Lei; Zhang, Cheng; Chuck, Roy S

2012-01-01

To evaluate the effect of the topical steroid, fluorometholone, and the non-steroidal anti-inflammatory drugs (NSAIDs), nepafenac and ketorolac, on inflammatory cytokine expression of the ocular surface in the botulium toxin B-induced murine dry eye model. Topical artificial tears (0.5% carboxymethylcellulose sodium), 0.1% fluorometholone, 0.1% nepafenac, and 0.4% ketorolac were applied 3 times per day in a dry eye mouse model 1 week after intralacrimal botulium toxin B (BTX-B) or saline (sham) injection. Tear production and corneal fluorescein staining were evaluated in all groups before injection at baseline and at 3 time points up to 4 weeks after injection. The pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were evaluated by immunohistochemistry. BTX-B-injected mice showed significantly decreased aqueous tear production and increased corneal fluorescein staining at the 1 and 2 week time points compared with normal control and saline-injected mice. In the BTX-B-injected mice, immunofluorescent staining for TNF-α and IL-1β in corneal and conjunctival epithelial cells increased significantly at the 2 and 4 week time points compared to that of normal and saline-injected mice, and returned to normal levels at the 4 week time point. Topical fluorometholone significantly improved corneal surface staining in the BTX-B-injected mice after 1 week of treatment, and increased the tear production within 2 weeks, but without statistical significant difference. Topical fluorometholone significantly decreased the staining of TNF-α and IL-1β in corneal and conjunctival epithelia after 1-week treatment. Topical artificial tears, 0.1% nepafenac, and 0.4% ketorolac did not show obvious effects on tear production, corneal surface staining, and levels of IL-1β and TNF-α expression in normal, and BTX-B-injected dry eye mice. Topical fluorometholone caused suppression of inflammatory cytokine expression on the ocular surface in the Botulium toxin B-induced murine dry eye model, while topical NSAIDs demonstrated no clearly beneficial effects.

A method for the direct injection and analysis of small volume human blood spots and plasma extracts containing high concentrations of organic solvents using revered-phase 2D UPLC/MS.

PubMed

Rainville, Paul D; Simeone, Jennifer L; Root, Dan S; Mallet, Claude R; Wilson, Ian D; Plumb, Robert S

2015-03-21

The emergence of micro sampling techniques holds great potential to improve pharmacokinetic data quality, reduce animal usage, and save costs in safety assessment studies. The analysis of these samples presents new challenges for bioanalytical scientists, both in terms of sample processing and analytical sensitivity. The use of two dimensional LC/MS with, at-column-dilution for the direct analysis of highly organic extracts prepared from biological fluids such as dried blood spots and plasma is demonstrated. This technique negated the need to dry down and reconstitute, or dilute samples with water/aqueous buffer solutions, prior to injection onto a reversed-phase LC system. A mixture of model drugs, including bromhexine, triprolidine, enrofloxacin, and procaine were used to test the feasibility of the method. Finally an LC/MS assay for the probe pharmaceutical rosuvastatin was developed from dried blood spots and protein-precipitated plasma. The assays showed acceptable recovery, accuracy and precision according to US FDA guidelines. The resulting analytical method showed an increase in assay sensitivity of up to forty fold as compared to conventional methods by maximizing the amount loaded onto the system and the MS response for the probe pharmaceutical rosuvastatin from small volume samples.

Water injected fuel cell system compressor

DOEpatents

Siepierski, James S.; Moore, Barbara S.; Hoch, Martin Monroe

2001-01-01

A fuel cell system including a dry compressor for pressurizing air supplied to the cathode side of the fuel cell. An injector sprays a controlled amount of water on to the compressor's rotor(s) to improve the energy efficiency of the compressor. The amount of water sprayed out the rotor(s) is controlled relative to the mass flow rate of air inputted to the compressor.

Scoping Studies to Evaluate the Benefits of an Advanced Dry Feed System on the Use of Low-Rank Coal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rader, Jeff; Aguilar, Kelly; Aldred, Derek

2012-11-30

This report describes the development of the design of an advanced dry feed system that was carried out under Task 4.0 of Cooperative Agreement DE-FE0007902 with the US DOE, “Scoping Studies to Evaluate the Benefits of an Advanced Dry Feed System on the use of Low- Rank Coal.” The resulting design will be used for the advanced technology IGCC case with 90% carbon capture for sequestration to be developed under Task 5.0 of the same agreement. The scope of work covered coal preparation and feeding up through the gasifier injector. Subcomponents have been broken down into feed preparation (including grindingmore » and drying), low pressure conveyance, pressurization, high pressure conveyance, and injection. Pressurization of the coal feed is done using Posimetric1 Feeders sized for the application. In addition, a secondary feed system is described for preparing and feeding slag additive and recycle fines to the gasifier injector. This report includes information on the basis for the design, requirements for down selection of the key technologies used, the down selection methodology and the final, down selected design for the Posimetric Feed System, or PFS.« less

Low damage dry etch for III-nitride light emitters

NASA Astrophysics Data System (ADS)

Nedy, Joseph G.; Young, Nathan G.; Kelchner, Kathryn M.; Hu, Yanling; Farrell, Robert M.; Nakamura, Shuji; DenBaars, Steven P.; Weisbuch, Claude; Speck, James S.

2015-08-01

We have developed a dry etch process for the fabrication of lithographically defined features close to light emitting layers in the III-nitride material system. The dry etch was tested for its effect on the internal quantum efficiency of c-plane InGaN quantum wells using the photoluminescence of a test structure with two active regions. No change was observed in the internal quantum efficiency of the test active region when the etched surface was greater than 71 nm away. To demonstrate the application of the developed dry etch process, surface-etched air gaps were fabricated 275 nm away from the active region of an m-plane InGaN/GaN laser diode and served as the waveguide upper cladding. Electrically injected lasing was observed without the need for regrowth or recovery anneals. This dry etch opens up a new design tool that can be utilized in the next generation of GaN light emitters.

Systemic delivery of parathyroid hormone (1-34) using inhalation dry powders in rats.

PubMed

Codrons, Valérie; Vanderbist, Francis; Verbeeck, Roger K; Arras, Mohammed; Lison, Dominique; Préat, Véronique; Vanbever, Rita

2003-05-01

The aim of this work was to prepare and characterize inhalation dry powders of human parathyroid hormone (PTH), as well as to assess their efficacy for systemic delivery of the peptide and safety in rats. The powders were prepared by spray-drying using PTH, sugars, dipalmitoylphosphatidylcholine, and/or albumin. They presented an average primary particle diameter of 4.5 microm and tap density of 0.06 g/cm(3), a mass median aerodynamic diameter between 3.9 and 5.9 microm, and reached up to 98% emitted dose and up to 61% fine particle fraction in the multi-stage liquid impinger using a Spinhaler inhaler device. Varying the airflow rate from 30 to 100 L/min had limited influence on the aerodynamic behavior of the aerosols. The absolute PTH bioavailability was 21% after intratracheal administration of the powder formed of PTH/albumin/lactose/dipalmitoylphosphatidylcholine and 18% after subcutaneous injection in rats. Equilibrium dialysis revealed a 78% binding of PTH to albumin and the withdrawal of albumin from the powder increased absolute bioavailability after inhalation from 21 to 34%. No acute inflammation appeared in the lung up to 48 h after a single inhalation. The increased bioavailability of the optimized powder aerosol of PTH makes it a promising alternative to subcutaneous injection. Copyright 2003 Wiley-Liss, Inc. and the American Pharmaceutical Association J Pharm Sci 92:938-950, 2003

Initiation and Activation of Faults in Dry and Wet Rock by Fluid Injection

NASA Astrophysics Data System (ADS)

Stanchits, S.; Mayr, S.; Shapiro, S. A.; Dresen, G.

2008-12-01

We studied fracturing of rock samples induced by water injection in axial compression tests on cylindrical specimens of Flechtingen sandstone and Aue granite of 50 mm diameter and 105-125 mm length. Samples were intact solid rock cylinders and cylinders with central boreholes of 5 mm diameter and 52 mm length or through-boreholes of 2.5 mm diameter. To monitor acoustic emissions (AE) and ultrasonic velocities, twelve P-wave and six polarized S-wave sensors were glued to the cylindrical surface of the rock. Full waveforms were stored in a 12 channel transient recording system (PROEKEL, Germany). Polarity of AE first motion was used to discriminate source types associated with tensile, shear and pore-collapse cracking. To monitor strain, two pairs of orthogonally oriented strain-gages were glued onto the specimen surface. Samples were deformed in two consecutive loading steps: 1) Initial triaxial loading was performed at 20-50 MPa confining pressure on dry (under vacuum) or fully saturated samples until the yield point was reached. 2) In a second stage distilled water was injected into the samples with pore pressure increasing up to 20 MPa. For saturated samples the pore pressure was increased in steps and in periodic pulses. Injection of water into dry porous sandstone resulted in propagation of an AE hypocenter cloud closely linked to propagation of the water front. Position of the migrating water front was estimated from ultrasonic velocity measurements and measurements of the injected water volume. Propagation rate of AE-induced cloud parallel to bedding was higher than perpendicular to bedding, possibly related to permeability anisotropy. Nucleation of a brittle shear fault occurred at a critical pore pressure level with a nucleation patch located at the central borehole. Micro-structural analysis of fractured samples shows excellent agreement between location of AE hypocenters and macroscopic faults.

Manufacture of modified milk protein concentrate utilizing injection of carbon dioxide.

PubMed

Marella, Chenchaiah; Salunke, P; Biswas, A C; Kommineni, A; Metzger, L E

2015-06-01

Dried milk protein concentrate is produced from skim milk using a combination of processes such as ultrafiltration (UF), evaporation or nanofiltration, and spray drying. It is well established that dried milk protein concentrate (MPC) that contains 80% (MPC80) and greater protein content (relative to dry matter) can lose solubility during storage as a result of protein-protein interactions and formation of insoluble complexes. Previous studies have shown that partial replacement of calcium with sodium improves MPC80 functionality and prevents the loss in solubility during storage. Those studies have used pH adjustment with the addition of acids, addition of monovalent salts, or ion exchange treatment of UF retentate. The objective of this study was to use carbon dioxide to produce MPC80 with improved functionality. In this study, reduced-calcium MPC80 (RCMPC) was produced from skim milk that was subjected to injection of 2,200 ppm of CO2 before UF, along with additional CO2 injection at a flow rate of 1.5 to 2 L/min during UF. A control MPC80 (CtrlMPC) was also produced from the same lot of skim milk without injection of CO2. The above processes were replicated 3 times, using different lots of skim milk for each replication. All the UF retentates were spray dried using a pilot-scale dryer. Skim milk and UF retentates were tested for ζ-potential (net negative charge), particle size, and viscosity. All the MPC were stored at room (22±1°C) and elevated (40°C) temperatures for 6 mo. Solubility was measured by dissolving the dried MPC in water at 22°C and at 10°C (cold solubility). Injection of CO2 and the resultant solubilization of calcium phosphate had a significant effect on UF performance, resulting in 10 and 20% loss in initial and average flux, respectively. Processing of skim milk with injection of CO2 also resulted in higher irreversible fouling resistances. Compared with control, the reduced-calcium MPC had 28 and 34% less ash and calcium, respectively. Injection of CO2 resulted in a significant decrease in ζ-potential and a significant increase in the size of the casein micelle. Moreover, RCMPC had a significantly higher solubility after storage at room temperature and at elevated temperature. This study demonstrates that MPC80 with a reduced calcium and mineral content can be produced with injection of CO2 before and during UF of skim milk. Copyright © 2015 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Improved Wear Resistance of Low Carbon Steel with Plasma Melt Injection of WC Particles

NASA Astrophysics Data System (ADS)

Liu, Aiguo; Guo, Mianhuan; Hu, Hailong

2010-08-01

Surface of a low carbon steel Q235 substrate was melted by a plasma torch, and tungsten carbide (WC) particles were injected into the melt pool. WC reinforced surface metal matrix composite (MMC) was synthesized. Dry sliding wear behavior of the surface MMC was studied and compared with the substrate. The results show that dry sliding wear resistance of low carbon steel can be greatly improved by plasma melt injection of WC particles. Hardness of the surface MMC is much higher than that of the substrate. The high hardness lowers the adhesion and abrasion of the surface MMC, and also the friction coefficient of it. The oxides formed in the sliding process also help to lower the friction coefficient. In this way, the dry sliding wear resistance of the surface MMC is greatly improved.

Stability Studies of a Freeze-Dried Recombinant Human Epidermal Growth Factor Formulation for Wound Healing.

PubMed

Santana, Héctor; García, Gerardo; Vega, Maribel; Beldarraín, Alejandro; Páez, Rolando

2015-01-01

We report on the stability assessment of a recombinant human epidermal growth factor (rhEGF) freeze-dried formulation for wound healing by intra-lesional injections. The suitability of packaging material for the light protection of finished dried powder was evaluated after stressed exposure conditions. Degradation kinetics of powder for injection was investigated at concentrations of 25-250 μg/vial and temperatures of 45, 60, and 70 °C. The long-term stability was evaluated after storage at 25 ± 2 °C/60 ± 5% relative humidity (6 months) and 2-8 °C (24 months) in the dark and analyzed at several time points. The stability after reconstitution with various diluents was also assessed after 24 h storage at 2-8 °C. The rhEGF samples were analyzed for structural integrity by reversed-phase high-performance liquid chromatography (RP-HPLC), size-exclusion HPLC, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Biological activity was investigated by measuring the cell proliferation in a murine fibroblast cell line. Results show that freeze-dried rhEGF in primary packaging only was photosensitive, as degradation by RP-HPLC that was completely suppressed by the secondary carton package was revealed. An increase in freeze-dried rhEGF stability was observed with the increase in protein concentration from 25 to 250 μg/vial. The long-term stability study showed no significant rhEGF degradation or physical change within the freeze-dried formulations containing 25 or 250 μg/vial of rhEGF. No physical, chemical or biological changes were observed for rhEGF after reconstitution in water for injection or 0.9% sodium chloride during the storage conditions studied. The stability of a recombinant human epidermal growth factor (rhEGF) freeze-dried formulation for wound healing by intra-lesional injections was assessed. The suitability of packaging material for the light protection of finished dried powder was evaluated after stressed exposure conditions. Degradation kinetics of powder for injection was investigated at concentrations of 25-250 μg/vial and temperatures of 45, 60, and 70 °C. The accelerated, long-term, and reconstitution stabilities were examined according to ICH guidelines for their utility time. The stability of rhEGF samples was analyzed by different chemical, physical, and biological activity assays. Results show that freeze-dried rhEGF in primary packaging only was photosensitive, as degradation by reversed-phase high performance liquid chromatography that was completely suppressed by the secondary carton package was revealed. An increase in freeze-dried rhEGF stability was observed with the increase in protein concentration. No significant rhEGF degradation or physical changes were observed within the freeze-dried formulations after 6 months storage at 25 ± 2 °C/60 ± 5% relative humidity or 24 months storage at 2-8 °C. No physical, chemical, or biological changes were observed for rhEGF after reconstitution in water for injection or 0.9% sodium chloride after 24 h storage at 2-8 °C. © PDA, Inc. 2015.

Real-time monitoring of moisture levels in wound dressings in vitro: an experimental study.

PubMed

McColl, David; Cartlidge, Brian; Connolly, Patricia

2007-10-01

Retaining an appropriate level of moisture at the interface between a healing wound and an applied dressing is considered to be critical for effective wound healing. Failure to control exudate at this interface can result in maceration or drying out of the wound surface. The ability to control moisture balance at the wound interface is therefore a key aspect of wound dressing performance. To date it has not been possible to monitor in any effective manner the distribution of moisture within dressings or how this varies with time. A new measurement system is presented based on sensors placed at the wound/dressing interface which are capable of monitoring moisture levels in real time. The system comprises a model wound bed and sensor array complete with fluid injection path to mimic exudate flow. Eight monitoring points, situated beneath the test dressing, allow the moisture profile across the complete dressing to be measured both during and after fluid injection. The system has been used to evaluate the performance of four foam dressings, a composite hydrofibre dressing and a film dressing. Stark contrasts in the performance of the wound contact layer were found between the different wound dressing types. The composite hydrofibre dressing retained moisture at the wound interface throughout the experiments while areas of the foam dressing quickly became dry, even during constant injection of fluid. The abundance of sensors allowed a moisture map of the surface of the wound dressing to be constructed, illustrating that the moisture profile was not uniform across several of the dressings tested during absorption and evaporation of liquid. These results raise questions as to how the dressings behave on a wound in vivo and indicate the need for a similar clinical monitoring system for tracking wound moisture levels.

40 CFR Table 3 to Subpart Jjjjjj... - Operating Limits for Boilers With Emission Limits

Code of Federal Regulations, 2013 CFR

2013-07-01

... as defined in § 63.11237. 4. Dry sorbent or activated carbon injection control Maintain the 30-day rolling average sorbent or activated carbon injection rate at or above the minimum sorbent injection rate or minimum activated carbon injection rate as defined in § 63.11237. When your boiler operates at...

System and process for biomass treatment

DOEpatents

Dunson, Jr., James B; Tucker, III, Melvin P; Elander, Richard T; Lyons, Robert C

2013-08-20

A system including an apparatus is presented for treatment of biomass that allows successful biomass treatment at a high solids dry weight of biomass in the biomass mixture. The design of the system provides extensive distribution of a reactant by spreading the reactant over the biomass as the reactant is introduced through an injection lance, while the biomass is rotated using baffles. The apparatus system to provide extensive assimilation of the reactant into biomass using baffles to lift and drop the biomass, as well as attrition media which fall onto the biomass, to enhance the treatment process.

Comparative studies on exenatide-loaded poly (D,L-lactic-co-glycolic acid) microparticles prepared by a novel ultra-fine particle processing system and spray drying.

PubMed

Zhu, Chune; Huang, Ying; Zhang, Xiaoying; Mei, Liling; Pan, Xin; Li, Ge; Wu, Chuanbin

2015-08-01

The purpose of this study was to compare the properties of exenatide-loaded poly (D,L-lactic-co-glycolic acid) microparticles (Ex-PLGA-MPs) prepared by a novel ultra-fine particle processing system (UPPS) and spray drying. UPPS is a proprietary technology developed by our group based on the disk rotation principle. Characteristics of the MPs including morphology, particle size distribution, drug content, encapsulation efficiency and in vitro release were comparatively studied. Cytotoxicity of the MPs was examined on A549 cells and the pharmacodynamics was investigated in vivo in type 2 diabetes Sprague-Dawley (SD) rats. Ex-PLGA-MPs prepared by UPPS showed larger particle size, denser surface, greater encapsulation efficiency, less initial burst release, and stable sustained release for more than one month in vitro as compared with the spray drying MPs. Meanwhile, the UPPS MPs effectively controlled the body growth rate and blood glucose in diabetes rats for at least three weeks after a single injection, while the spray drying MPs showed effective control period of about two weeks. UPPS technology was demonstrated to manufacture Ex-PLGA-MPs as a potential sustained release protein/polypeptide delivery system, which is an alternative method for the most commonly used spray drying. This comparative research provides a new guidance for microparticle preparation technology. Copyright © 2015 Elsevier B.V. All rights reserved.

Comparison Between Botulinum Toxin A Injection and Lacrimal Punctal Plugs for the Control of Post-LASIK Dry Eye Manifestations: A Prospective Study.

PubMed

Fouda, Sameh M; Mattout, Hala K

2017-06-01

Laser in situ keratomelieusis (LASIK) is one of the commonest refractive procedures performed nowadays. The dry eye problem is nearly universal in all patients after LASIK and it can be so annoying that the post-operative patient satisfaction is sometimes precluded. Conventional treatment includes the use of artificial tears. Alternative methods such as punctal plugs and botulinum toxin injection can be used for the management of post-LASIK dry eye. The aim of this study is to compare botulinum toxin injection in the orbicularis muscle to lacrimal punctal plugs for the control of post-LASIK dry eye manifestations. This is a prospective study that included 60 patients who had LASIK eye surgery for correction of refractive errors. Patients were randomly assigned to one of three methods of dry eye management; the first method was conventional medical treatment with preservative-free tear substitutes only (group A: 20 patients = 40 eyes); the second method was intraoperative injection of botulinum toxin A (BTA) in the orbicularis muscle below the lower punctum of both eyes (group B: 20 patients = 40 eyes) and the third method was intraoperative insertion of temporary extended duration silicone punctal plugs in the lower punctum of both eyes (group C: 20 patients = 40 eyes). In the first follow-up visit after 2 weeks, the two test groups (B, C) showed a statistically significant increase in both tear film break up time (TBUT) and Schirmer test score with a decrease in the OSDI score and daily frequency of lubricants used in comparison to the control group A. These differences were maintained in the next follow-up visit but they became statistically insignificant at the 3rd and 6th post-operative months. Complications were encountered more in the punctal plug patients (60%) than in BTA patients (25%) and this difference was statistically significant. The use of BTA injection to control dry eye symptoms by inducing temporary punctal ectropion is an effective method to improve patient satisfaction after LASIK eye surgery. It has higher level of patient satisfaction and fewer complications in comparison to punctal plugs or topical standard dry eye treatment.

40 CFR 60.2680 - What if I do not use a wet scrubber, fabric filter, activated carbon injection, selective...

Code of Federal Regulations, 2014 CFR

2014-07-01

..., fabric filter, activated carbon injection, selective noncatalytic reduction, an electrostatic... use a wet scrubber, fabric filter, activated carbon injection, selective noncatalytic reduction, an... reduction, fabric filter, an electrostatic precipitator, or a dry scrubber or limit emissions in some other...

40 CFR 60.2680 - What if I do not use a wet scrubber, fabric filter, activated carbon injection, selective...

Code of Federal Regulations, 2013 CFR

2013-07-01

..., fabric filter, activated carbon injection, selective noncatalytic reduction, an electrostatic... use a wet scrubber, fabric filter, activated carbon injection, selective noncatalytic reduction, an... reduction, fabric filter, an electrostatic precipitator, or a dry scrubber or limit emissions in some other...

40 CFR Table 6 to Subpart Jjjjjj... - Establishing Operating Limits

Code of Federal Regulations, 2014 CFR

2014-07-01

... Dry sorbent or activated carbon injection rate operating parameters Establish a site-specific minimum sorbent or activated carbon injection rate operating limit according to § 63.11211(b) Data from the sorbent or activated carbon injection rate monitors and the mercury performance stack tests (a) You must...

40 CFR Table 6 to Subpart Jjjjjj... - Establishing Operating Limits

Code of Federal Regulations, 2013 CFR

2013-07-01

... Dry sorbent or activated carbon injection rate operating parameters Establish a site-specific minimum sorbent or activated carbon injection rate operating limit according to § 63.11211(b) Data from the sorbent or activated carbon injection rate monitors and the mercury performance stack tests (a) You must...

Effect of a single injection of cabergoline at dry off on udder characteristics in high-yielding dairy cows.

PubMed

Bertulat, S; Isaka, N; de Prado, A; Lopez, A; Hetreau, T; Heuwieser, W

2017-04-01

In recent years, relationships between high milk yield at dry off, higher prevalence for new intramammary infections, and stress were evaluated. Considering increasing milk yield, dry off methods need to be refined to ensure udder health and animal welfare, especially in high-yielding dairy cows. The present work evaluated the effect of a single cabergoline injection (Velactis, Ceva Santé Animale, Libourne, France) at dry off on udder pressure, milk leakage, and signs of udder pain after dry off. A total of 234 high-yielding (≥16 kg of milk/d) dairy cows was enrolled 7 d before and followed up until 14 d after dry off. Cows were dried off without preparation (i.e., no feed change or intermittent milking before dry off) and treated with a single i.m. injection of 5.6 mg of cabergoline (n = 115) or placebo (n = 119) after last milking. Udder characteristics were measured 4 d before (i.e., before and after milking) and 1, 2, 3, 7, 10, and 14 d after dry off. Udder pressure was evaluated utilizing a hand-held dynamometer. Milk leakage and signs of udder pain were noted as binary variables. Whereas udder pressure baseline values after last milking did not differ between treatment groups (0.541 ± 0.15 kg), cabergoline significantly reduced udder pressure in primiparous but not in multiparous cows after dry off. Differences between cabergoline- and placebo-treated primiparous cows could be evaluated until 3 d after dry off. The first day after dry off, udder pressure in placebo- and cabergoline-treated cows increased by 115% and 42.3%, respectively. Whereas pressure values in placebo cows were highest on the first day after dry off (1.16 ± 0.61 kg) and slowly decreased afterward, udder pressure in cows treated with cabergoline had a slower increase and peak only 2 d after dry off (0.94 ± 0.44 kg). Furthermore, cabergoline caused a reduction of milk leakage, a known factor for new intramammary infections. Only 11.3% of cows treated with cabergoline showed milk leakage compared with 21.0% placebo-treated cows. Additionally, cows with placebo treatment were 2.8 times as likely to show signs of udder pain compared with cows treated with cabergoline. An effect of cabergoline on udder pressure, milk leakage, and udder pain was limited to the first week after dry off. Our data provide evidence that a single injection of cabergoline reduces risk factors for udder health and animal welfare problems around dry off in high-yielding dairy cows with more than 16 kg of milk/d. Further research is warranted, however, to investigate if cabergoline at dry off can also be used to reduce new intramammary infection rates and improve animal welfare after dry off. The Authors. Published by the Federation of Animal Science Societies and Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells

PubMed Central

Kwon, Kwang-Chul; Verma, Dheeraj; Singh, Nameirakpam D.; Herzog, Roland; Daniell, Henry

2012-01-01

Among 12 billion injections administered annually, unsafe delivery leads to >20 million infections and >100 million reactions. In an emerging new concept, freeze-dried plant cells (lettuce) expressing vaccine antigens/biopharmaceuticals are protected in the stomach from acids/enzymes but are released to the immune or blood circulatory system when plant cell walls are digested by microbes that colonize the gut. Vaccine antigens bioencapsulated in plant cells upon oral delivery after priming, conferred both mucosal and systemic immunity and protection against bacterial, viral or protozoan pathogens or toxin challenge. Oral delivery of autoantigens was effective against complications of type 1diabetes and hemophilia, by developing tolerance. Oral delivery of proinsulin or exendin-4 expressed in plant cells regulated blood glucose levels similar to injections. Therefore, this new platform offers a low cost alternative to deliver different therapeutic proteins to combat infectious or inherited diseases by eliminating inactivated pathogens, expensive purification, cold storage/transportation and sterile injections. PMID:23099275

Spatial resolution of transport parameters in a subtropical karst conduit system during dry and wet seasons

NASA Astrophysics Data System (ADS)

Ender, Anna; Goeppert, Nadine; Goldscheider, Nico

2018-04-01

Karst aquifers are characterized by a high degree of hydrologic variability and spatial heterogeneity of transport parameters. Tracer tests allow the quantification of these parameters, but conventional point-to-point experiments fail to capture spatiotemporal variations of flow and transport. The goal of this study was to elucidate the spatial distribution of transport parameters in a karst conduit system at different flow conditions. Therefore, six tracer tests were conducted in an active and accessible cave system in Vietnam during dry and wet seasons. Injections and monitoring were done at five sites along the flow system: a swallow hole, two sites inside the cave, and two springs draining the system. Breakthrough curves (BTCs) were modeled with CXTFIT software using the one-dimensional advection-dispersion model and the two-region nonequilibrium model. In order to obtain transport parameters in the individual sections of the system, a multi-pulse injection approach was used, which was realized by using the BTCs from one section as input functions for the next section. Major findings include: (1) In the entire system, mean flow velocities increase from 183 to 1,043 m/h with increasing discharge, while (2) the proportion of immobile fluid regions decrease; (3) the lowest dispersivity was found at intermediate discharge; (4) in the individual cave sections, flow velocities decrease along the flow direction, related to decreasing gradients, while (5) dispersivity is highest in the middle section of the cave. The obtained results provide a valuable basis for the development of an adapted water management strategy for a projected water-supply system.

40 CFR Table 3 to Subpart Jjjjjj... - Operating Limits for Boilers With Emission Limits

Code of Federal Regulations, 2012 CFR

2012-07-01

... performance test demonstrating compliance with the PM emission limitation. 4. Dry sorbent or carbon injection control Maintain the sorbent or carbon injection rate at or above the lowest 2-hour average sorbent flow... emissions limitation. When your boiler operates at lower loads, multiply your sorbent or carbon injection...

Toward intradermal vaccination: preparation of powder formulations by collapse freeze-drying.

PubMed

Etzl, Elsa E; Winter, Gerhard; Engert, Julia

2014-03-01

Intradermal powder immunization is an emerging technique in vaccine delivery. The purpose of this study was to generate powder particles for intradermal injection by freeze-drying and subsequent cryo-milling. Two different freeze-drying protocols were compared, a moderate freeze-drying cycle and an aggressive freeze-drying cycle, which induced a controlled collapse of the sugar matrix. Ovalbumin served as model antigen. The influence of collapse drying and cryo-milling on particle morphology and protein stability was investigated. Cryo-milling generated irregularly shaped particles of size 20-70 µm. The recovery of soluble monomer of ovalbumin was not changed during freeze-drying and after cryo-milling, or after 12 months of storage at 2-8 °C. A slight increase in higher molecular weight aggregates was found in formulations containing the polymer dextran after 12 months of storage at 50 °C. Light obscuration measurements showed an increase in cumulative particle counts after cryo-milling that did not further increase during storage at 2-8 °C for 12 months. The applicability of the cryo-milling process to other therapeutic proteins was shown using recombinant human granulocyte-colony stimulating factor. Collapse freeze-drying and subsequent cryo-milling allows the generation of particles suitable for intradermal powder injection.

Monitoring CO2 invasion processes at the pore scale using geological labs on chip.

PubMed

Morais, S; Liu, N; Diouf, A; Bernard, D; Lecoutre, C; Garrabos, Y; Marre, S

2016-09-21

In order to investigate at the pore scale the mechanisms involved during CO2 injection in a water saturated pore network, a series of displacement experiments is reported using high pressure micromodels (geological labs on chip - GLoCs) working under real geological conditions (25 < T (°C) < 75 and 4.5 < p (MPa) < 8). The experiments were focused on the influence of three experimental parameters: (i) the p, T conditions, (ii) the injection flow rates and (iii) the pore network characteristics. By using on-chip optical characterization and imaging approaches, the CO2 saturation curves as a function of either time or the number of pore volume injected were determined. Three main mechanisms were observed during CO2 injection, namely, invasion, percolation and drying, which are discussed in this paper. Interestingly, besides conventional mechanisms, two counterintuitive situations were observed during the invasion and drying processes.

Survey of flue gas desulfurization systems: Hawthorn Station, Kansas City Power and Light Co. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Isaacs, G.A.; Zada, F.K.

1975-09-01

Results are given of a survey of the flue gas desulfurization (FGD) systems at Kansas City Power and Light Co.'s Hawthorn Power Station. The FGD systems on Hawthorn boilers 3 and 4 were designed to operate by injection of dry limestone in the boiler's furnace, followed by tail gas scrubbing. Because of tube plugging in boiler 4 the mode of operation of the FGD system on that boiler was modified: ground limestone is now introduced into the flue gas near the gas inlet to the scrubber tower. (GRA)

Enhancing the use of coals by gas reburning-sorbent injection: Volume 3 -- Gas reburning-sorbent injection at Edwards Unit 1, Central Illinois Light Company. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1996-03-01

Design work has been completed for a Gas Reburning-Sorbent Injection (GR-SI) system to reduce emissions of NO{sub x} and SO{sub 2} from a wall fired unit at Central Illinois Light Company`s Edwards Station Unit 1, located in Bartonville, Illinois. The goal of the project was to reduce emissions of NO{sub x} by 60%, from the as found baseline of 0.98 lb/MBtu and to reduce emissions of SO{sub 2} by 50%. Since the unit currently fires a blend of high sulfur Illinois coal and low sulfur Kentucky coal to meet an SO{sub 2} limit of 1.8 lb/MBtu, the goal at thismore » site was amended to meeting this limit while increasing the fraction of high sulfur coal to 57% from the current 15% level. GR-SI requires injection of natural gas into the furnace at the level of the top burner row, creating a fuel-rich zone in which NO{sub x} formed in the coal zone is reduced to N{sub 2}. Recycled flue gas is used to increase the reburning fuel jet momentum, resulting in enhanced mixing. Recycled flue gas is also used to cool the top row of burners which would not be in service during GR operation. Dry hydrated lime sorbent is injected into the upper furnace to react with SO{sub 2}, forming solid CaSO{sub 4} and CaSO{sub 3}, which are collected by the ESP. The system was designed to inject sorbent at a rate corresponding to a calcium (sorbent) to sulfur (coal) molar ratio of 2.0. The SI system design was optimized with respect to gas temperature, injection air flow rate, and sorbent dispersion. Sorbent injection air flow is equal to 3% of the combustion air. The design includes modifications of the ESP, sootblowing, and ash handling systems.« less

Subsurface storage of freshwater in south Florida; a prospectus

USGS Publications Warehouse

Merritt, M.L.; Meyer, F.W.; Sonntag, W.H.; Fitzpatrick, D.J.

1983-01-01

A method of increasing storage capacity for freshwater in south Florida is to use brackish artesian aquifers as reservoirs. In this way, water deficiencies occurring during the annual dry season can be offset by surplus water obtained during the wet season and injected underground. Most of south Florida is underlain by several deep, confined, carbonate waterbearing zones which might be suitable for freshwater storage. These zones are in the Avon Park, Ocala, Suwannee, Tampa, and Hawthorn Formations. Experimental freshwater injection systems have been operated at five locations with promising, but not fully definitive, results. A determination of the feasibility of freshwater injection at a selected site begins with an assessment of the local geologic suitability. Verification of feasibility, however, requires injection and recovery tests to be performed at the site. Recovery efficiency, a measure of the success of the operation, is the amount of potable water, expressed as a percentage of the volume injected, which can be recovered before its salinity, or the concentration of other chemical constituents present in the native aquifer water, increases to the point that the recovered water is no longer useable. (USGS)

Capreomycin oleate microparticles for intramuscular administration: Preparation, in vitro release and preliminary in vivo evaluation.

PubMed

Cambronero-Rojas, Adrián; Torres-Vergara, Pablo; Godoy, Ricardo; von Plessing, Carlos; Sepúlveda, Jacqueline; Gómez-Gaete, Carolina

2015-07-10

Capreomycin sulfate (CS) is a second-line drug used for the treatment of multidrug-resistant tuberculosis (MDR-TB). The adverse effects profile and uncomfortable administration scheme of CS has led to the development of formulations based on liposomes and polymeric microparticles. However, as CS is a water-soluble peptide that does not encapsulate properly into hydrophobic particulate matrices, it was necessary to reduce its aqueous solubility by forming the pharmacologically active capreomycin oleate (CO) ion pair. The aim of this research was to develop a new formulation of CO for intramuscular injection, based on biodegradable microparticles that encapsulate CO in order to provide a controlled release of the drug with reduced local and systemic adverse effects. The CO-loaded microparticles prepared by spray drying or solvent emulsion-evaporation were characterized in their morphology, encapsulation efficiency, in vitro/in vivo kinetics and tissue tolerance. Through scanning electron microscopy it was confirmed that the microparticles were monodisperse and spherical, with an optimal size for intramuscular administration. The interaction between CO and the components of the microparticle matrix was confirmed on both formulations by X-ray powder diffraction and differential scanning calorimetry analyses. The encapsulation efficiencies for the spray-dried and emulsion-evaporation microparticles were 92% and 56%, respectively. The in vitro kinetics performed on both formulations demonstrated a controlled and continuous release of CO from the microparticles, which was successfully reproduced on an in vivo rodent model. The results of the histological analysis demonstrated that none of the formulations produced significant tissue damage on the site of injection. Therefore, the results suggest that injectable CO microparticles obtained by spray drying and solvent emulsion-evaporation could represent an interesting therapeutic alternative for the treatment of MDR-TB. Copyright © 2015 Elsevier B.V. All rights reserved.

40 CFR Table 8 to Subpart Ddddd of... - Demonstrating Continuous Compliance

Code of Federal Regulations, 2012 CFR

2012-07-01

...(c). 5. Dry Scrubber Sorbent or Carbon Injection Rate a. Collecting the sorbent or carbon injection... the data to 3-hour block averages; and c. Maintaining the 3-hour average sorbent or carbon injection... established during the performance test according to §§ 63.7530(c). 7. Fuel Pollutant Content a. Only burning...

40 CFR Table 8 to Subpart Ddddd of... - Demonstrating Continuous Compliance

Code of Federal Regulations, 2010 CFR

2010-07-01

...(c). 5. Dry Scrubber Sorbent or Carbon Injection Rate a. Collecting the sorbent or carbon injection... the data to 3-hour block averages; and c. Maintaining the 3-hour average sorbent or carbon injection... established during the performance test according to §§ 63.7530(c). 7. Fuel Pollutant Content a. Only burning...

40 CFR Table 8 to Subpart Ddddd of... - Demonstrating Continuous Compliance

Code of Federal Regulations, 2011 CFR

2011-07-01

...(c). 5. Dry Scrubber Sorbent or Carbon Injection Rate a. Collecting the sorbent or carbon injection... the data to 3-hour block averages; and c. Maintaining the 3-hour average sorbent or carbon injection... established during the performance test according to §§ 63.7530(c). 7. Fuel Pollutant Content a. Only burning...

Stabilization of active matter by flow-vortex lattices and defect ordering

PubMed Central

Doostmohammadi, Amin; Adamer, Michael F.; Thampi, Sumesh P.; Yeomans, Julia M.

2016-01-01

Active systems, from bacterial suspensions to cellular monolayers, are continuously driven out of equilibrium by local injection of energy from their constituent elements and exhibit turbulent-like and chaotic patterns. Here we demonstrate both theoretically and through numerical simulations, that the crossover between wet active systems, whose behaviour is dominated by hydrodynamics, and dry active matter where any flow is screened, can be achieved by using friction as a control parameter. Moreover, we discover unexpected vortex ordering at this wet–dry crossover. We show that the self organization of vortices into lattices is accompanied by the spatial ordering of topological defects leading to active crystal-like structures. The emergence of vortex lattices, which leads to the positional ordering of topological defects, suggests potential applications in the design and control of active materials. PMID:26837846

[The protection of hydrogen-rich saline on a rat dry eye model induced by scopolamine hydrobromide].

PubMed

Chu, Y Y; Hua, N; Ru, Y S; Zhao, S Z

2017-05-11

Objective: To evaluate the effect of hydrogen-rich saline (HRS) on dry eye rats induced by subcutaneous injection of scopolamine hydrobromide. Methods: Experiment research. Thirty female Wistar rats at about six weeks old were randomly divided into the normal group, dry eye group, HRS eyedrops group, normal saline eyedrops group (NS), HRS intraperitoneal injection group and NS intraperitoneal injection group, with 5 rats in each group. The dry eye was induced by subcutaneous injection of scopolamine hydrobromide in the latter five groups. The clinical signs of dry eye such as tear volume (SⅠt), tear break-up time (BUT) and corneal epithelial fluorescein staining scores were evaluated on day 7, 14, 21 and 28. On the 28th day, ten eyes in each group were enucleated and processed for paraffin sections for HE, PAS and immunohistochemistry stainings. Analysis of variance was used to test the data, and independent samples t -test was used for comparison between the two groups. Two-way repeated measure ANOVA was used to compare the difference among groups at different time points, one-way ANOVA was used to test the comparisons of the clinical signs at one time, and LSD was used to for comparison between two groups. Results: Before and after the experiment of the day 7, 14, 21, 28, the values of SIt in HRS eyedrops group and HRS intraperitoneal injection group were respectively:(3.625±1.157),(3.313±0.704),(3.250±0.535),(3.313±0.372), (3.375±0.582)mm and (3.500±1.019), (2.893±0.656), (3.321±0.668), (3.179±0.575), (3.214±0.871)mm. The values of BUT were respectively: (2.750±0.707), (2.688±0.594), (2.813±0.753), (3.000±0.756), (2.750±0.707)s and (3.000±0.679), (2.321±0.464), (2.750±0.753), (3.214±0.699), (2.679±0.608)s. The values of fluorescein staining score were respectively: (6.250±0.707), (8.875±0.641), (8.750±0.707), (9.250±0.463), (8.250±1.282) and (6.000±0.679), (9.143±1.027), (8.857±0.770), (9.143±0.949), (8.500±0.760). The difference of SIt, BUT and fluorescein staining score between the groups was statistically significant on the 14th day( F= 5.194, 3.894, 16.487, P< 0.05), the values of SⅠt, BUT and fluorescein staining score of HRS eyedrops group showed significantly better than NS eyedrops group( P< 0.05), HRS intraperitoneal injection group showed significantly better than NS intraperitoneal injection group( P< 0.05), and there was no significant difference between HRS eyedrops group and HRS intraperitoneal injection group( P> 0.05), which remained stable until the day 28. On the 28(th) day, HE, PAS and immunohistochemistry stainings showed the corneal and conjunctival epithelia became smooth and regular, the cell layer number decreased, the tissue hyperplasia and edema were lightened, and the size of goblet cells tended to be normal, and the squamous metaplasia and inflammation were relieved. In HRS eyedrops group and HRS intraperitoneal injection group. Conclusions: HRS eyedrops group and HRS intraperitoneal injection group can relieve the signs of dry eye, improve the pathological damage of cornea and conjunctiva, and protect the ocular surface of a rat dry eye model, which is better than NS groups from the 14(th) day. (Chin J Ophthalmol, 2017, 53: 363-372 ) .

Injection of Super-Critical CO2 in Brine Saturated Sandstone:

NASA Astrophysics Data System (ADS)

Ott, Holger; de Kloe, Kees; Taberner, Conxita; Marcelis, Fons; Makurat, Axel

2010-05-01

Presently, large-scale geological sequestration of CO2, originating from sources like fossil-fueled power plants and contaminated gas production, is seen as an option to reduce anthropogenic emission of greenhouse gases to the atmosphere. Deep saline aquifers and depleted oil and gas fields are potential subsurface deposits for CO2. Injected CO2, however, interacts physically and chemically with the formation leading to uncertainties for CCS projects. One of these uncertainties is related to a dry-out zone that is likely to form around the well bore owing to the injection of dry CO2. Precipitation of salt (mainly halite) that is associated with that drying out of a saline formation has the potential to impair injectivity, and could even lead to the loss of a well. If dry (or under-saturated), super-critical (SC) CO2 is injected into water-bearing geological formations like saline aquifers, water is removed by either advection of the aqueous phase or by evaporation of water and subsequent advection in the injected CO2-rich phase. Both mechanisms act in parallel, however while advection of the aqueous phase decreases with increasing CO2 saturation (diminished mobility), evaporation becomes increasingly important as the aqueous phase becomes immobile. Below residual water saturation, only evaporation takes place and the formation dries out if no additional source of water is available. If water evaporates, the salts originally present in the water are left behind. In case of highly saline formations, the amount of salt that potentially precipitates per unit volume can be quite substantial. It depends on salinity, the solubility limit of water in the CO2 rich phase, and on the ratio of advection and evaporation rates. Since saturations and flow rates cover a large range as functions of space and time close to the well bore, there is no easy answer to the questions whether, where and how salt precipitation impacts injectivity. The present paper presents results of core-flood experiments that were performed to investigate the spatial and temporal precipitation of salt due to the injection of dry CO2 and to understand the underlying mechanisms; super-critical CO2 was injected into brine-saturated sandstone (Berea) samples under realistic pressure and temperature conditions and at high injection rate. To match flow rates that are realistic for the near well-bore area, the experiments were performed on small-scale samples with a cross section of less than 1 cm2. Density profiles were measured by mCT (micro computer tomography) scanning during injection. Reference scans and brine doping with a contrast agent allow the distinction between the CO2-rich phase, the aqueous phase and precipitated solid salt even on pore scale. By means of mCT scanning, spatial and time evolution of halite precipitation in rock samples have been observed under sequestration conditions. Pattern formation of solid salt along the main flow direction as well as a cross-sectional pattern formation has been found. However, while there are areas of high local solid salt accumulation, permeability remained unaffected, which might be a result of the precipitation pattern. The results were complemented by (ex-situ) eSEM/EDAX measurements to study where and how salt precipitates on the microscopic scale. The SEM results cannot be directly translated to in-situ conditions, as salt migrates post-experiment at ambient conditions, but give valuable insight into microscopic processes controlling deposition. Numerical simulations have been performed for a qualitative understanding of principle mechanisms and show a dependency of the observed profile on injection rate and capillary pressure.

MERCURY CONTROL IN MUNICIPAL WASTE COMBUSTORS AND COAL-FIRED UTILITIES

EPA Science Inventory

Control of mercury (Hg) emissions from municipal waste combustors (MWCs) and coal-fired utilities has attracted attention due to current and potential regulations. Among several techniques evaluated for Hg control, dry sorbent injection (primarily injection of activated carbon) h...

Fracturing of porous rock induced by fluid injection

NASA Astrophysics Data System (ADS)

Stanchits, Sergei; Mayr, Sibylle; Shapiro, Serge; Dresen, Georg

2011-04-01

We monitored acoustic emission (AE) activity and brittle failure initiated by water injection into initially dry critically stressed cylindrical specimens of Flechtingen sandstone of 50 mm diameter and 105-125 mm length. Samples were first loaded in axial direction at 40-50 MPa confining pressure at dry conditions close to peak stress. Subsequently distilled water was injected either at the bottom of specimen or via a central borehole at pore pressures of 5-30 MPa. Water injection into stressed porous sandstone induced a cloud of AE events located close to the migrating water front. Water injection was monitored by periodic ultrasonic velocity measurements across the sample. Propagation of the induced cloud of AE was faster in the direction parallel to bedding than normal to it, indicating permeability anisotropy. Water injection was associated with significant AE activity demonstrating increased contribution of tensile source type. Brittle failure was accompanied by increased contribution of shear and pore collapse source types. At a critical pore pressure, a brittle fault nucleated from a cloud of induced AE events in all samples. Microstructural analysis of fractured samples shows excellent agreement between location of AE hypocenters and macroscopic faults.

40 CFR 63.8540 - What parts of my plant does this subpart cover?

Code of Federal Regulations, 2010 CFR

2010-07-01

... retrofitting with a dry lime injection fabric filter (DIFF), dry lime scrubber/fabric filter (DLS/FF), or wet... fired product to meet the relevant standards by retrofitting with a DIFF, DLS/FF, or WS. (g) An affected...

Encapsulation of antigen-loaded silica nanoparticles into microparticles for intradermal powder injection.

PubMed

Deng, Yibin; Mathaes, Roman; Winter, Gerhard; Engert, Julia

2014-10-15

Epidermal powder immunisation (EPI) is being investigated as a promising needle-free delivery methods for vaccination. The objective of this work was to prepare a nanoparticles-in-microparticles (nano-in-micro) system, integrating the advantages of nanoparticles and microparticles into one vaccine delivery system for epidermal powder immunisation. Cationic mesoporous silica nanoparticles (MSNP-NH2) were prepared and loaded with ovalbumin as a model antigen. Loading was driven by electrostatic interactions. Ovalbumin-loaded silica nanoparticles were subsequently formulated into sugar-based microparticles by spray-freeze-drying. The obtained microparticles meet the size requirement for EPI. Confocal microscopy was used to demonstrate that the nanoparticles are homogeneously distributed in the microparticles. Furthermore, the silica nanoparticles in the dry microparticles can be re-dispersed in aqueous solution showing no aggregation. The recovered ovalbumin shows integrity compared to native ovalbumin. The present nano-in-micro system allows (1) nanoparticles to be immobilized and finely distributed in microparticles, (2) microparticle formation and (3) re-dispersion of nanoparticles without subsequent aggregation. The nanoparticles inside microparticles can (1) adsorb proteins to cationic shell/surface voids in spray-dried products without detriment to ovalbumin stability, (2) deliver antigens in nano-sized modes to allow recognition by the immune system. Copyright © 2014 Elsevier B.V. All rights reserved.

The effect of seasons on behaviour during milking in buffaloes ( Bos bubalis)

NASA Astrophysics Data System (ADS)

Gangwar, P. C.

1982-06-01

An investigation on behaviour during milking involving 200 buffaloes was carried out to study the effect of climate on milking behaviour for a period of four years. The results obtained were: (1) In extremely docile animals (temperament score I) the mean distribution was least (33.5) in hot-dry summer as compared to winter (39.2) and hot-humid summer. (2) The number of buffaloes milked after oxytocin injections was maximum (11.1%) during the hot-dry summer against other seasons for the temperament score I over temperament scores III and IV, where all buffaloes were milked with oxytocin injections. (3) The mean flow rate was least in hot-dry summer in each temperament score. (4) Milking time was higher in all the temperament scored buffaloes during the hot-dry summer than during the other seasons. It is concluded that as environmental temperature increases, there occurs an increase in thermal stress, the milking behaviour changes and animals become more hostile and excited which leads to a decrease in milk production.

An injectable hybrid nanoparticle-in-oil-in-water submicron emulsion for improved delivery of poorly soluble drugs

NASA Astrophysics Data System (ADS)

Wang, Shuo; Wang, Hua; Liang, Wenquan; Huang, Yongzhuo

2012-04-01

Poor drugability problems are commonly seen in a class of chemical entities with poor solubility in water and oil, and moreover, physicochemical instability of these compounds poses extra challenges in design of dosage forms. Such problems contribute a significant high failure rate in new drug development. A hybrid nanoparicle-in-oil-in-water (N/O/W) submicron emulsion was proposed for improved delivery of poorly soluble and unstable drugs (e.g., dihydroartemisinin (DHA)). DHA is known for its potent antimalarial effect and antitumor activity. However, its insolubility and instability impose big challenges for formulations, and so far, no injectable dosage forms are clinically available yet. Therefore, an injectable DHA N/O/W system was developed. Unlike other widely-explored systems (e.g., liposomes, micelles, and emulsions), in which low drug load and only short-term storage are often found, the hybrid submicron emulsion possesses three-fold higher drug-loading capacity than the conventional O/W emulsion. Of note, it can be manufactured into a freeze-drying form and can render its storage up to 6 months even in room temperature. The in vivo studies demonstrated that the PK profiles were significantly improved, and this injectable system was effective in suppressing tumor growth. The strategy provides a useful solution to effective delivery of such a class of drugs.

Dry coating of solid dosage forms: an overview of processes and applications.

PubMed

Foppoli, Anastasia Anna; Maroni, Alessandra; Cerea, Matteo; Zema, Lucia; Gazzaniga, Andrea

2017-12-01

Dry coating techniques enable manufacturing of coated solid dosage forms with no, or very limited, use of solvents. As a result, major drawbacks associated with both organic solvents and aqueous coating systems can be overcome, such as toxicological, environmental, and safety-related issues on the one hand as well as costly drying phases and impaired product stability on the other. The considerable advantages related to solventless coating has been prompting a strong research interest in this field of pharmaceutics. In the article, processes and applications relevant to techniques intended for dry coating are analyzed and reviewed. Based on the physical state of the coat-forming agents, liquid- and solid-based techniques are distinguished. The former include hot-melt coating and coating by photocuring, while the latter encompass press coating and powder coating. Moreover, solventless techniques, such as injection molding and three-dimensional printing by fused deposition modeling, which are not purposely conceived for coating, are also discussed in that they would open new perspectives in the manufacturing of coated-like dosage forms.

Assessing the Use of Dry Wells as a Tool for Stormwater Management and Groundwater Recharge in Urban Areas

NASA Astrophysics Data System (ADS)

Edwards, E.; Harter, T.; Fogg, G. E.; Washburn, B.; Bryson, R.; Meirovitz, C.; Fawcett, J.; Kretsinger Grabert, V. J.; Bowles, C.; Carr, M.; Nelson, C.

2014-12-01

Dry wells are gravity-fed, excavated pits with perforated casings used to facilitate stormwater infiltration and groundwater recharge in areas comprised primarily of impermeable surfaces or low permeability soils. Stormwater runoff that would otherwise be routed to streams or drains in urban areas is used as a source of aquifer recharge. However, the potential for groundwater contamination caused by urban runoff bypassing surface soil filtration has prevented more widespread use of dry wells as a recharge mechanism. We present the results of a literature survey to assess the potential of dry wells for safe stormwater recharge. Dry wells have been inculpated in groundwater contamination events, although accusations were typically not backed by scientific data. In 1989 groundwater in Modesto, CA, was contaminated with tetrachloroethylene from a dry cleaning facility. The city had been using dry wells to manage stormwater for more than 50 years without detrimental impacts before the contamination. A USGS monitoring study proved that the contamination was from sewer system leakage, and did not involve the dry wells. Some areas of the country have used dry wells with positive results. The Underground Injection Control system (UICs) study in Portland, OR, has been active for ten years, and currently operates over 9,000 UICs. Initially, a ten foot separation distance was enforced between the seasonal high water table and the bottom perforation of the UIC; however, due to monitoring and modeling results that indicate the protectiveness of groundwater, this distance has been reduced to zero feet. Future work will include a comparative pilot study involving a residential and an industrial site in Elk Grove, CA. The study will use modeling tools to assess the recharge potential and groundwater protectiveness of dry wells. Both sites are outfitted with four monitoring wells each: an upgradient monitoring well, two downgradient monitoring wells, and a vadose zone monitoring well. The results of water quality sampling will determine the contaminants of interest, and a model of the fate and transport of these contaminants in the vadose zone will be coupled with hydraulic models to estimate the response of the hydrogeologic system to dry well recharge, with the goal of creating a tool that can be used to evaluate future dry well locations.

In vivo liver visualizations with magnetic particle imaging based on the calibration measurement approach

NASA Astrophysics Data System (ADS)

Dieckhoff, J.; Kaul, M. G.; Mummert, T.; Jung, C.; Salamon, J.; Adam, G.; Knopp, T.; Ludwig, F.; Balceris, C.; Ittrich, H.

2017-05-01

Magnetic particle imaging (MPI) facilitates the rapid determination of 3D in vivo magnetic nanoparticle distributions. In this work, liver MPI following intravenous injections of ferucarbotran (Resovist®) was studied. The image reconstruction was based on a calibration measurement, the so called system function. The application of an enhanced system function sample reflecting the particle mobility and aggregation status of ferucarbotran resulted in significantly improved image reconstructions. The finding was supported by characterizations of different ferucarbotran compositions with the magnetorelaxometry and magnetic particle spectroscopy technique. For instance, similar results were obtained between ferucarbotran embedded in freeze-dried mannitol sugar and liver tissue harvested after a ferucarbotran injection. In addition, the combination of multiple shifted measurement patches for a joint reconstruction of the MPI data enlarged the field of view and increased the covering of liver MPI on magnetic resonance images noticeably.

In vivo liver visualizations with magnetic particle imaging based on the calibration measurement approach.

PubMed

Dieckhoff, J; Kaul, M G; Mummert, T; Jung, C; Salamon, J; Adam, G; Knopp, T; Ludwig, F; Balceris, C; Ittrich, H

2017-05-07

Magnetic particle imaging (MPI) facilitates the rapid determination of 3D in vivo magnetic nanoparticle distributions. In this work, liver MPI following intravenous injections of ferucarbotran (Resovist ® ) was studied. The image reconstruction was based on a calibration measurement, the so called system function. The application of an enhanced system function sample reflecting the particle mobility and aggregation status of ferucarbotran resulted in significantly improved image reconstructions. The finding was supported by characterizations of different ferucarbotran compositions with the magnetorelaxometry and magnetic particle spectroscopy technique. For instance, similar results were obtained between ferucarbotran embedded in freeze-dried mannitol sugar and liver tissue harvested after a ferucarbotran injection. In addition, the combination of multiple shifted measurement patches for a joint reconstruction of the MPI data enlarged the field of view and increased the covering of liver MPI on magnetic resonance images noticeably.

Effects of Chamber Pressure and Partial Pressure of Water Vapor on Secondary Drying in Lyophilization.

PubMed

Searles, James A; Aravapalli, Sridhar; Hodge, Cody

2017-10-01

Secondary drying is the final step of lyophilization before stoppering, during which water is desorbed from the product to yield the final moisture content. We studied how chamber pressure and partial pressure of water vapor during this step affected the time course of water content of aqueous solutions of polyvinylpyrrolidone (PVP) in glass vials. The total chamber pressure had no effect when the partial pressure of water vapor was very low. However, when the vapor phase contained a substantial fraction of water vapor, the PVP moisture content was much higher. We carried out dynamic vapor sorption experiments (DVS) to demonstrate that the higher PVP moisture content was a straightforward result of the higher water vapor content in the lyophilizer. The results highlight that the partial pressure of water vapor is extremely important during secondary drying in lyophilization, and that lower chamber pressure set points for secondary drying may sometimes be justified as a strategy for ensuring low partial pressure of water vapor, especially for lyophilizers that do not inject dry gas to control pressure. These findings have direct application for process transfers/scale ups from freeze-dryers that do not inject dry gas for pressure control to those that do, and vice versa.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Levendis, Y.A.; Zhu, W.; Wise, D.L.

A fundamental study was conducted on the effectiveness of the chemical calcium magnesium acetate (CMA) as a sulfur capture agent during combustion of pulverized coal. It was based on high-temperature laboratory-bench experiments with the scope of exploring the use of CMA as a dry scrubbing'' medium for in-boiler injection. Two methods of CMA introduction in the furnace were considered: dry-spraying fine powders of the chemical and wet-spraying aqueous solutions to generate fine aerosols. It considered conditions pertinent to post-flame in-boiler injection of CMA to identify optimum temperatures and residence times. In addition to the versatility of the water-soluble CMA tomore » enable spray drying injection and therefore eliminate grinding costs, there are other attractive features. Mainly, its ability to form highly cenospheric, popcorn''-like, oxide particles on heating to high temperatures. These cenospheres possess thin, porous walls with blowholes that enable penetration of the SO[sub 2] in the interior of the particle which promotes high sorbent utilization. SO[sub 2] captures in the order of 90% were achieved with dry-injection of the chemical at furnace gas temperatures of about 1,000[degree]C, a Ca/S ratio of 2, and particle size of [approximately] 50[mu]m. Moreover, CMA was superior (by over 40%) to either CaCO[sub 3] or Ca(OH)[sub 2] in sulfur capture effectiveness per unit mass of calcium. This commercially obtained CMA was even superior to reagent-grade calcium acetate (by as much as 30%), again per unit mass of calcium. The utilization of CMA and calcium acetate depended on the cenosphere wall thickness, rather than the particle size and, thus, outperformed other sorbents regardless of the size of the resulting oxide particles.« less

Paralysis of the orbicularis muscle of the eye using botulinum toxin type A in the treatment for dry eye.

PubMed

Serna-Ojeda, Juan Carlos; Nava-Castaneda, Angel

2017-03-01

To evaluate the efficacy of botulinum toxin type A injection to cause orbicularis eyelid muscle paralysis to improve dry eye signs and symptoms. A prospective, randomized, comparative eye-to-eye and interventional study was performed. Patients with dry eye symptoms and positive fluorescein corneal staining were included. Randomly one eyelid received a subcutaneous injection of botulinum toxin in the medial orbicularis muscle portion of the lower eyelid, and the other eye received placebo. The subjective evaluation was achieved with a questionnaire assessing symptoms, quality of vision and ocular comfort level. The objective evaluation included the measurement of the tear film break-up time (TBUT), Schirmer's test and corneal and conjunctival staining. Twenty patients were included with a mean age of 59.5 years. Two weeks after the botulinum toxin injection, all patients showed a decrease in the horizontal movement of the lower eyelid when blinking. The eyes in the active treatment group showed better scores compared with the sham group in four symptoms 4 weeks after the treatment. The TBUT was higher at 1 and 3 months in the active treatment group. The corneal and conjunctival staining were significantly lower in the active treatment group at 1 and 3 months, and the Schirmer's test showed better measurements in the same group at 2 weeks, 1 month and 3 months. There were no adverse events reported. The injection of botulinum toxin A in the medial part of the lower eyelid is an effective and safe procedure that temporally improves some of the signs and symptoms of patients with dry eye. © 2016 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

A systematic review of different substance injection and dry needling for treatment of temporomandibular myofascial pain.

PubMed

Machado, E; Machado, P; Wandscher, V F; Marchionatti, A M E; Zanatta, F B; Kaizer, O B

2018-05-22

Temporomandibular myofascial pain presents a major challenge in the diagnosis of temporomandibular disorders (TMD). Due to the characteristics of this condition, intramuscular injection procedures are often needed for adequate control of symptoms and treatment. Thus, the aim of this systematic review was to evaluate the effectiveness of dry needling and injection with different substances in temporomandibular myofascial pain. Electronic databases PubMed, EMBASE, CENTRAL/Cochrane, Lilacs, Scopus, Web of Science and CAPES Catalog of Dissertations and Theses were searched for randomized clinical trials until January 2018. Manual search was performed in relevant journals and in the references/citations of the included studies. The selection of studies was carried out by two independent reviewers according to eligibility criteria. From 7128 eligible studies, 137 were selected for full-text analysis and 18 were included. Due to the heterogeneity of the primary studies it was not possible to perform a meta-analysis. The narrative analysis of the results showed that most of the studies had methodological limitations and biases that compromised the quality of the findings. Dry needling and local anaesthesic injections seem promising, but there is a need to conduct further randomized clinical trials, with larger samples and longer follow-up times, to evaluate the real effectiveness of the technique and evaluated substances. Copyright © 2018 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

An Accurate Full-flexion Anterolateral Portal for Needle Placement in the Knee Joint With Dry Osteoarthritis.

PubMed

Hussein, Mohamed

2017-07-01

Accurate delivery of an injection into the intra-articular space of the knee is achieved in only two thirds of knees when using the standard anterolateral portal. The use of a modified full-flexion anterolateral portal provides a highly accurate, less painful, and more effective method for reproducible intra-articular injection without the need for ultrasonographic or fluoroscopic guidance in patients with dry osteoarthritis of the knee. The accuracy of needle placement was assessed in a prospective series of 140 consecutive injections in patients with symptomatic degenerative knee arthritis without clinical knee effusion. Procedural pain was determined using the Numerical Rating Scale. The accuracy rates of needle placement were confirmed with fluoroscopic imaging to document the dispersion pattern of injected contrast material. Using the standard anterolateral portal, 52 of 70 injections were confirmed to have been placed in the intra-articular space on the first attempt (accuracy rate, 74.2%). Using the modified full-flexion anterolateral portal, 68 of 70 injections were placed in the intra-articular space on the first attempt (accuracy rate, 97.1%; P = 0.000). This study revealed that using the modified full-flexion anterolateral portal for injections into the knee joint resulted in more accurate and less painful injections than those performed by the same orthopaedic surgeon using the standard anterolateral portal. In addition, the technique offered therapeutic delivery into the joint without the need for fluoroscopic confirmation. Therapeutic Level II.

Sewage sludge dewatering using flowing liquid metals

DOEpatents

Carlson, Larry W.

1986-01-01

A method and apparatus for reducing the moisture content of a moist sewage sludge having a moisture content of about 50% to 80% and formed of small cellular micro-organism bodies having internally confined water is provided. A hot liquid metal is circulated in a circulation loop and the moist sewage sludge is injected in the circulation loop under conditions of temperature and pressure such that the confined water vaporizes and ruptures the cellular bodies. The vapor produced, the dried sludge, and the liquid metal are then separated. Preferably, the moist sewage sludge is injected into the hot liquid metal adjacent the upstream side of a venturi which serves to thoroughly mix the hot liquid metal and the moist sewage sludge. The venturi and the drying zone after the venturi are preferably vertically oriented. The dried sewage sludge recovered is available as a fuel and is preferably used for heating the hot liquid metal.

Investigation and demonstration of dry carbon-based sorbent injection for mercury control. Quarterly technical report, July 1, 1996--September 31, 1996

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hunt, T.; Sjostrom, S.; Smith, J.

1996-11-06

The overall objective of this two phase program is to investigate the use of dry carbon-based sorbents for mercury control. This information is important to the utility industry in anticipation of pending regulations. During Phase I, a bench-scale field test device that can be configured as an electrostatic precipitator, a pulse-jet baghouse, or a reverse-gas baghouse has been designed, built and integrated with an existing pilot-scale facility at PSCo`s Comanche Station. Up to three candidate sorbents will be injected into the flue gas stream upstream of the test device to and mercury concentration measurements will be made to determine themore » mercury removal efficiency for each sorbent. During the Phase II effort, component integration for the most promising dry sorbent technology shall be tested at the 5000 acfm pilot-scale.« less

Comparison between Local Injections of Silicone Oil and Hydrocortisone Acetate in Chronic Arthritis

PubMed Central

Corbett, Mary; Seifert, Martin H.; Hacking, Celia; Webb, Sylvia

1970-01-01

One of three preparations—silicone oil, hydrocortisone acetate, and hydrocortisone acetate plus saline—was injected into 22 osteoarthritic and 49 rheumatoid knees. Silicone injected into stiff, dry, grating knees which were the site of chronic arthritis did not promote better movement than did hydrocortisone alone. Hydrocortisone plus saline, however, appeared to be more effective than silicone or hydrocortisone alone in increasing movement in rheumatoid knees. The maximum increase in movement was found to occur three weeks after each of the three different injection preparations. PMID:5411599

BOILER DESIGN CRITERIA FOR DRY SORBENT SO2 CONTROL WITH LOW-NOX BURNERS: NEW UNIT APPLICATIONS

EPA Science Inventory

The report describes a study to define boiler modifications required to achieve 70% SO2 removal with sorbent injection on a large tangentially fired utility boiler without supplemental spray drying. The study is a follow on to a recently completed broader evaluation of boiler des...

Improving the dissolution properties of curcumin using dense gas antisolvent technology.

PubMed

Kurniawansyah, Firman; Quachie, Lisa; Mammucari, Raffaella; Foster, Neil R

2017-04-15

The dissolution properties of curcumin are notoriously poor and hinder its bioavailability. To improve its dissolution properties, curcumin has been formulated with methyl-β-cyclodextrin and polyvinylpyrrolidone by the atomized rapid injection solvent extraction (ARISE) system. The compounds were co-precipitated from organic solutions using carbon dioxide at 30°C and 95bar as the antisolvent. Curcumin formulations were also produced by physical mixing and freeze drying for comparative purposes. The morphology, crystallinity, solid state molecular interactions, apparent solubility and dissolution profiles of samples were observed. The results indicate that the ARISE process is effective in the preparation of curcumin micro-composites with enhanced dissolution profiles compared to unprocessed material and products from physical mixing and freeze drying. Copyright © 2017 Elsevier B.V. All rights reserved.

75 FR 76260 - Implantation or Injectable Dosage Form New Animal Drugs; Flunixin

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-08

... meglumine solution by intravenous injection in lactating dairy cows for control of pyrexia associated with... cows for control of pyrexia associated with acute bovine mastitis. The supplemental application is... for 36 hours after the last treatment must not be used for food. Do not use in dry dairy cows. A...

Protective Immunity in Mice Achieved with Dry Powder Formulation and Alternative Delivery of Plague F1-V Vaccine▿

PubMed Central

Huang, Joanne; D'Souza, Ajit J.; Alarcon, Jason B.; Mikszta, John A.; Ford, Brandi M.; Ferriter, Matthew S.; Evans, Michelle; Stewart, Todd; Amemiya, Kei; Ulrich, Robert G.; Sullivan, Vincent J.

2009-01-01

The potential use of Yersinia pestis as a bioterror agent is a great concern. Development of a stable powder vaccine against Y. pestis and administration of the vaccine by minimally invasive methods could provide an alternative to the traditional liquid formulation and intramuscular injection. We evaluated a spray-freeze-dried powder vaccine containing a recombinant F1-V fusion protein of Y. pestis for vaccination against plaque in a mouse model. Mice were immunized with reconstituted spray-freeze-dried F1-V powder via intramuscular injection, microneedle-based intradermal delivery, or noninvasive intranasal administration. By intramuscular injection, the reconstituted powder induced serum antibody responses and provided protection against lethal subcutaneous challenge with 1,000 50% lethal doses of Y. pestis at levels equivalent to those elicited by unprocessed liquid formulations (70 to 90% protection). The feasibility of intradermal and intranasal delivery of reconstituted powder F1-V vaccine was also demonstrated. Overall, microneedle-based intradermal delivery was shown to be similar in efficacy to intramuscular injection, while intranasal administration required an extra dose of vaccine to achieve similar protection. In addition, the results suggest that seroconversion against F1 may be a better predictor of protection against Y. pestis challenge than seroconversion against either F1-V or V. In summary, we demonstrate the preclinical feasibility of using a reconstituted powder F1-V formulation and microneedle-based intradermal delivery to provide protective immunity against plague in a mouse model. Intranasal delivery, while feasible, was less effective than injection in this study. The potential use of these alternative delivery methods and a powder vaccine formulation may result in substantial health and economic benefits. PMID:19261773

Protective immunity in mice achieved with dry powder formulation and alternative delivery of plague F1-V vaccine.

PubMed

Huang, Joanne; D'Souza, Ajit J; Alarcon, Jason B; Mikszta, John A; Ford, Brandi M; Ferriter, Matthew S; Evans, Michelle; Stewart, Todd; Amemiya, Kei; Ulrich, Robert G; Sullivan, Vincent J

2009-05-01

The potential use of Yersinia pestis as a bioterror agent is a great concern. Development of a stable powder vaccine against Y. pestis and administration of the vaccine by minimally invasive methods could provide an alternative to the traditional liquid formulation and intramuscular injection. We evaluated a spray-freeze-dried powder vaccine containing a recombinant F1-V fusion protein of Y. pestis for vaccination against plaque in a mouse model. Mice were immunized with reconstituted spray-freeze-dried F1-V powder via intramuscular injection, microneedle-based intradermal delivery, or noninvasive intranasal administration. By intramuscular injection, the reconstituted powder induced serum antibody responses and provided protection against lethal subcutaneous challenge with 1,000 50% lethal doses of Y. pestis at levels equivalent to those elicited by unprocessed liquid formulations (70 to 90% protection). The feasibility of intradermal and intranasal delivery of reconstituted powder F1-V vaccine was also demonstrated. Overall, microneedle-based intradermal delivery was shown to be similar in efficacy to intramuscular injection, while intranasal administration required an extra dose of vaccine to achieve similar protection. In addition, the results suggest that seroconversion against F1 may be a better predictor of protection against Y. pestis challenge than seroconversion against either F1-V or V. In summary, we demonstrate the preclinical feasibility of using a reconstituted powder F1-V formulation and microneedle-based intradermal delivery to provide protective immunity against plague in a mouse model. Intranasal delivery, while feasible, was less effective than injection in this study. The potential use of these alternative delivery methods and a powder vaccine formulation may result in substantial health and economic benefits.

Determination of Parabens by Injection-Port Derivatization Coupled With Gas-Chromatography-Mass Spectrometry and Matrix Solid Phase Dispersion

NASA Astrophysics Data System (ADS)

Djatmika, Rosalina; Ding, Wang-Hsien; Sulistyarti, Hermin

2018-01-01

A rapid determination of four parabens preservatives (methyl paraben, ethyl paraben, propyl paraben, and butyl paraben) in marketed seafood is presented. Analytes were extracted and purified using matrix solid-phase dispersion (MSPD) method, followed by Injection port acylation gas chromatography-mass spectrometry (GC-MS) with acetic anhydride reagent. In this method, acylation of parabens was performed by acetic anhydride at GC injection-port generating reduction of the time-consuming sample-processing steps, and the amount of toxic reagents and solvents. The parameters affecting this method such as injection port temperature, purge-off time and acylation (acetic anhydride) volume were studied. In addition, the MSPD influence factors (including the amount of dispersant and clean-up co-sorbent, as well as the volume of elution solvent) were also investigated. After MSPD method and Injection port acylation applied, good linearity of analytes was achieved. The limits of quantitation (LOQs) were 0.2 to 1.0 ng/g (dry weight). Compared with offline derivatization commonly performed, injection port acylation employs a rapid, simple, low-cost and environmental-friendly derivatization process. The optimized method has been successfully applied for the analysis of parabens in four kind of marketed seafood. Preliminary results showed that the total concentrations of four selected parabens ranged from 16.7 to 44.7 ng/g (dry weight).

Improved silicon carbide for advanced heat engines. I - Process development for injection molding

NASA Technical Reports Server (NTRS)

Whalen, Thomas J.; Trela, Walter

1989-01-01

Alternate processing methods have been investigated as a means of improving the mechanical properties of injection-molded SiC. Various mixing processes (dry, high-sheer, and fluid) were evaluated along with the morphology and particle size of the starting beta-SiC powder. Statistically-designed experiments were used to determine significant effects and interactions of variables in the mixing, injection molding, and binder removal process steps. Improvements in mechanical strength can be correlated with the reduction in flaw size observed in the injection molded green bodies obtained with improved processing methods.

Barium selenate supplementation and its effect on intramammary infection in pasture-based dairy cows.

PubMed

Ceballos, A; Kruze, J; Barkema, H W; Dohoo, I R; Sanchez, J; Uribe, D; Wichtel, J J; Wittwer, F

2010-04-01

A significant proportion of cattle receive inadequate dietary Se because of its low content in soils and pastures of various regions of the world. Several economically important diseases in dairy cows, such as mastitis, have been associated with Se deficiency. The objective of this study was to evaluate the effect of a single injection of a long-acting form of Se at drying off on the risk and incidence rate of new intramammary infections and on milk somatic cell count in the subsequent lactation in pasture-based dairy cows. Forty-nine Chilean Holstein-Friesian cows were fed a diet containing <0.05 mg of Se/kg of ration dry matter. During the dry period, cows were allocated to 1 of 2 groups, a supplemented (n=24) group treated with a single subcutaneous injection of barium selenate 2 mo before calving and a control group (n=25) that remained unsupplemented. Duplicate foremilk samples were aseptically collected within 6 d after calving and every 2 wk until drying-off for bacteriological culture. Milk samples were also collected monthly for somatic cell count evaluation. Blood samples were collected before treatment and at 30, 90, 180, and 270 d after treatment for analysis of blood glutathione peroxidase (GPx) activity. The activity of glutathione peroxidase was higher in supplemented cows 30 d after the injection until the end of the study. The risk and incidence rate of new intramammary infections was not affected by supplementation. A progressive increase in somatic cell count was observed throughout lactation, but there was no effect of supplementation. In conclusion, a one-time injection of barium selenate 2 mo before calving in these pasture-based dairy cows did not affect udder health in the subsequent lactation, indicating that Se basal intake was adequate for preventing subclinical mastitis in pasture-based cows in southern Chile. Copyright (c) 2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Long-Term Outcomes in Patients with Neovascular Age-Related Macular Degeneration Who Maintain Dry Macula after Three Monthly Ranibizumab Injections.

PubMed

Kim, Kyung Min; Kim, Jae Hui; Chang, Young Suk; Kim, Jong Woo; Kim, Chul Gu

2018-01-01

To evaluate long-term changes in visual acuity and retinal microstructure in patients with neovascular age-related macular degeneration (AMD) who had maintained dry macula after initial treatment. This retrospective observational study included 55 eyes that were diagnosed with neovascular AMD, were treated with three monthly ranibizumab injections, and maintained dry macula during a two-year follow-up. Best-corrected visual acuity (BCVA) at three months and at the final follow-up were compared, and the degree of visual improvement was compared between eyes with and without improvement of the ellipsoid zone. In addition, the incidence of improvement of the ellipsoid zone was compared between eyes with different extents of disruption. The mean follow-up period was 30.3 ± 4.1 months. BCVA at three months and at the final follow-up was 0.51 ± 0.46 and 0.45 ± 0.49 (P<0.001). Among 35 eyes that exhibited >200 μm of disruption of the ellipsoid zone, 15 (42.9%) showed improvement of the ellipsoid zone, and the improvement in BCVA was greater in these eyes than that in the remaining 20 eyes (P=0.021). A higher incidence of improvement of the ellipsoid zone was noted in eyes with 200 to 800 μm of disruption than in eyes with >800 μm of disruption (P<0.001). Long-term improvement in visual acuity was noted in eyes that had maintained dry macula after three ranibizumab injections. The status of the ellipsoid zone at three months was closely associated with visual improvement.

Comparison of two methods for composite score generation in dry eye syndrome.

PubMed

See, Craig; Bilonick, Richard A; Feuer, William; Galor, Anat

2013-09-19

To compare two methods of composite score generation in dry eye syndrome (DES). Male patients seen in the Miami Veterans Affairs eye clinic with normal eyelid, corneal, and conjunctival anatomy were recruited to participate in the study. Patients filled out the Dry Eye Questionnaire 5 (DEQ5) and underwent measurement of tear film parameters. DES severity scores were generated by independent component analysis (ICA) and latent class analysis (LCA). A total of 247 men were included in the study. Mean age was 69 years (SD 9). Using ICA analysis, osmolarity was found to carry the largest weight, followed by eyelid vascularity and meibomian orifice plugging. Conjunctival injection and tear breakup time (TBUT) carried the lowest weights. Using LCA analysis, TBUT was found to be best at discriminating healthy from diseased eyes, followed closely by Schirmer's test. DEQ5, eyelid vascularity, and conjunctival injection were the poorest at discrimination. The adjusted correlation coefficient between the two generated composite scores was 0.63, indicating that the shared variance was less than 40%. Both ICA and LCA produced composite scores for dry eye severity, with weak to moderate agreement; however, agreement for the relative importance of single diagnostic tests was poor between the two methods.

Electrochemical immunoassay on a microfluidic device with sequential injection and flushing functions.

PubMed

Nashida, Norihiro; Satoh, Wataru; Fukuda, Junji; Suzuki, Hiroaki

2007-06-15

An integrated microfluidic device with injecting, flushing, and sensing functions was realized using valves that operate based on direct electrowetting. The device consisted of two substrates: a glass substrate with driving and sensing electrodes and a poly(dimethylsiloxane) (PDMS) substrate. Microfluidic transport was achieved using the spontaneous movement of solutions in hydrophilic flow channels formed with a dry-film photoresist layer. The injection and flushing of solutions were controlled by gold working electrodes, which functioned as valves. The valves were formed either in the channels or in a through-hole in the glass substrate. To demonstrate the system's applicability to an immunoassay, the detection of immobilized antigens was performed as a partial simulation of a sandwich immunoassay. Human alpha-fetoprotein (AFP) or an anti-human AFP antibody was immobilized on a platinum working electrode in the chamber using a plasma-polymerized film (PPF). By applying a potential to the injection valves, necessary solutions were injected one by one through the channels into a reaction chamber at the center of the chip and incubated for reasonable periods of time. The solutions were then flushed through the flushing valve and absorbed in a filter paper placed under the device. After incubation with the corresponding antibodies labeled with glucose oxidase (GOD), electrochemical detection was conducted. In both cases, the obtained current depended on the amount of immobilized antigen. The calibration curves were sigmoidal, and the detection limit was 0.1 ng. The developed microfluidic system could potentially be a fundamental component for a micro immunoassay of the next generation.

Enhancing the use of coals by gas reburning-sorbent injection. Volume 3, Gas reburning-sorbent injection at Edwards Unit 1, Central Illinois Light Company

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1994-10-01

Design work has been completed for a Gas Reburning-Sorbent Injection (GR-SI) system to reduce emissions of NO{sub x}, and SO{sub 2} from a wall fired unit. A GR-SI system was designed for Central Illinois Light Company`s Edwards Station Unit 1, located in Bartonville, Illinois. The unit is rated at 117 MW(e) (net) and is front wall fired with a pulverized bituminous coal blend. The goal of the project was to reduce emissions of NO{sub x} by 60%, from the ``as found`` baseline of 0.98 lb/MBtu (420 mg/MJ), and to reduce emissions of S0{sub 2} by 50%. Since the unit currentlymore » fires a blend of high sulfur Illinois coal and low sulfur Kentucky coal to meet an S0{sub 2} limit Of 1.8 lb/MBtu (770 mg/MJ), the goal at this site was amended to meeting this limit while increasing the fraction of high sulfur coal to 57% from the current 15% level. GR-SI requires injection of natural gas into the furnace at the level of the top burner row, creating a fuel-rich zone in which NO{sub x} formed in the coal zone is reduced to N{sub 2}. The design natural gas input corresponds to 18% of the total heat input. Burnout (overfire) air is injected at a higher elevation to burn out fuel combustible matter at a normal excess air level of 18%. Recycled flue gas is used to increase the reburning fuel jet momentum, resulting in enhanced mixing. Recycled flue gas is also used to cool the top row of burners which would not be in service during GR operation. Dry hydrated lime sorbent is injected into the upper furnace to react with S0{sub 2}, forming solid CaSO{sub 4} and CaSO{sub 3}, which are collected by the ESP. The SI system design was optimized with respect to gas temperature, injection air flow rate, and sorbent dispersion. Sorbent injection air flow is equal to 3% of the combustion air. The design includes modifications of the ESP, sootblowing, and ash handling systems.« less

Development of an advanced combined iodine dispenser/detector. [for spacecraft water supplies

NASA Technical Reports Server (NTRS)

Lantz, J. B.; Jensen, F. C.; Winkler, H. E.; Schubert, F. A.

1977-01-01

Injection of iodine into water is widely used to control microbial growth. An entirely automated device for I2 injection has been developed for spacecraft application. Transfer of I2 into the water from a concentrated form is controlled electrochemically via feedback from an integrated photometric I2 level detector. All components are contained within a package weighing only 1.23 kg (2.7 lb) dry, which occupies only 1213 cu cm (74 cu in) of space, and which has the capacity to iodinate 10,900 kg (24,000 lb) of water of 5 ppm. These features exceed design specifications. The device performed satisfactorily during extended testing at variable water flow rates and temperatures. Designed to meet specifications of the Shuttle Orbiter, the device will find application in the regenerative water systems of advanced spacecraft.

Enhanced scratching evoked by PAR-2 agonist and 5-HT but not histamine in a mouse model of chronic dry skin itch.

PubMed

Akiyama, T; Carstens, M Iodi; Carstens, E

2010-11-01

Chronic itch is a symptom of many skin conditions and systemic disease, and it has been hypothesized that the chronic itch may result from sensitization of itch-signaling pathways. We induced experimental chronic dry skin on the rostral back of mice, and observed a significant increase in spontaneous hindlimb scratches directed to the dry skin. Spontaneous scratching was significantly attenuated by a PAR-2 antibody and 5-HT2A receptor antagonist, indicating activation of these receptors by endogenous mediators released under dry skin conditions. We also observed a significant increase in the number of scratch bouts evoked by acute intradermal injections of a protease-activated receptor (PAR)-2 agonist and serotonin (5-HT), but not histamine. We additionally investigated if pruritogen-evoked activity of dorsal root ganglion (DRG) neurons is enhanced in this model. DRG cells from dry skin mice exhibited significantly larger responses to the PAR-2 agonist and 5-HT, but not histamine. Spontaneous scratching may reflect ongoing itch, and enhanced pruritogen-evoked scratching may represent hyperknesis (enhanced itch), both potentially due to sensitization of itch-signaling neurons. The correspondence between enhanced behavioral scratching and DRG cell responses suggest that peripheral pruriceptors that respond to proteases and 5-HT, but not histamine, may be sensitized in dry skin itch. Copyright © 2010 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

Pilot scale-SO{sub 2} control by dry sodium bicarbonate injection and an electrostatic precipitator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pliat, M.J.; Wilder, J.M.

2007-10-15

A 500 actual cubic feet gas per minute (acfm) pilot-scale SO{sub 2} control study was undertaken to investigate flue gas desulfurization (FGD) by dry sodium sorbents in 400{sup o}F (204.5{sup o}C) flue gases emitted from a coal fired boiler with flue gas concentrations between 350 and 2500 ppm SO{sub 2}. Powdered sodium alkaline reagents were injected into the hot flue gas downstream of the air preheater and the spent reagents were collected using an electrostatic precipitator. Three different sorbents were used: processed sodium bicarbonate of two particle sizes; solution mined sodium bicarbonate, and processed sodium sesquicarbonate. SO{sub 2} concentrations weremore » measured upstream of the reagent injection, 25-ft (7.62 m) downstream of the injection point, and downstream of the electrostatic precipitator. SO{sub 2} collection efficiencies ranged from 40 to 80% using sodium bicarbonate stoichiometric ratios from 0.5 to 3.0. Much of the in-duct SO{sub 2} removal occurred during the first second of reagent reaction time, indicating that the sulfur dioxide-sodium reaction rates may be faster than have been measured for fixed bed measurements reported in the literature.« less

Enhanced responses of lumbar superficial dorsal horn neurons to intradermal PAR-2 agonist but not histamine in a mouse hindpaw dry skin itch model

PubMed Central

Akiyama, Tasuku; Carstens, Mirela Iodi

2011-01-01

Chronic itch is symptomatic of many skin conditions and systemic diseases. Little is known about pathophysiological alterations in itch-signaling neural pathways associated with chronic itch. We used a mouse model of hindpaw chronic dry skin itch to investigate properties of presumptive itch-signaling neurons. Neurons in the lumbar superficial dorsal horn ipsilateral to hindpaw dry skin treatment exhibited a high level of spontaneous activity that was inhibited by scratching the plantar surface. Most spontaneously active units exhibited further increases in firing rate following intradermal injection of an agonist of the protease-activated receptor PAR-2, or histamine. The large majority of pruritogen-responsive units also responded to capsaicin and allyl isothiocyanate. For neurons ipsilateral to dry skin treatment, responses elicited by the PAR-2 agonist, but not histamine or mechanical stimuli, were significantly larger compared with neurons ipsilateral to vehicle (water) treatment or neurons recorded in naïve (untreated) mice. The spontaneous activity may signal ongoing itch, while enhanced PAR-2 agonist-evoked responses may underlie hyperknesis (enhanced itch), both of which are symptomatic of many chronic itch conditions. The enhancement of neuronal responses evoked by the PAR-2 agonist, but not by histamine or mechanical stimuli, implies that the dry skin condition selectively sensitized PAR-2 agonist-sensitive primary afferent pruriceptors. PMID:21430273

Enhanced responses of lumbar superficial dorsal horn neurons to intradermal PAR-2 agonist but not histamine in a mouse hindpaw dry skin itch model.

PubMed

Akiyama, Tasuku; Carstens, Mirela Iodi; Carstens, E

2011-06-01

Chronic itch is symptomatic of many skin conditions and systemic diseases. Little is known about pathophysiological alterations in itch-signaling neural pathways associated with chronic itch. We used a mouse model of hindpaw chronic dry skin itch to investigate properties of presumptive itch-signaling neurons. Neurons in the lumbar superficial dorsal horn ipsilateral to hindpaw dry skin treatment exhibited a high level of spontaneous activity that was inhibited by scratching the plantar surface. Most spontaneously active units exhibited further increases in firing rate following intradermal injection of an agonist of the protease-activated receptor PAR-2, or histamine. The large majority of pruritogen-responsive units also responded to capsaicin and allyl isothiocyanate. For neurons ipsilateral to dry skin treatment, responses elicited by the PAR-2 agonist, but not histamine or mechanical stimuli, were significantly larger compared with neurons ipsilateral to vehicle (water) treatment or neurons recorded in naïve (untreated) mice. The spontaneous activity may signal ongoing itch, while enhanced PAR-2 agonist-evoked responses may underlie hyperknesis (enhanced itch), both of which are symptomatic of many chronic itch conditions. The enhancement of neuronal responses evoked by the PAR-2 agonist, but not by histamine or mechanical stimuli, implies that the dry skin condition selectively sensitized PAR-2 agonist-sensitive primary afferent pruriceptors.

Pharmacokinetic-pharmacodynamic study of subcutaneous injection of depot nandrolone decanoate using dried blood spots sampling coupled with ultrapressure liquid chromatography tandem mass spectrometry assays.

PubMed

Singh, Gurmeet K S; Turner, Leo; Desai, Reena; Jimenez, Mark; Handelsman, David J

2014-07-01

Testosterone (T) and nandrolone (N) esters require deep im injections by medical personnel but these often deposit injectate into sc fat so that more convenient sc self-administration may be feasible. To investigate the feasibility and pharmacology of sc injection of N decanoate in healthy men using dried blood spot (DBS) for frequent blood sampling without clinic visits. Healthy male volunteers received 100 mg N decanoate by a single sc injection. Finger-prick capillary blood was spotted onto filter paper before injection daily at home for 21 d and stored at room temperature. Venous whole blood was also spotted onto filter paper before and weekly for 3 wk after injection. DBS were extracted for assay of N and T by liquid chromatography tandem mass spectrometry in a single batch with serum concentrations estimated with adjustment for capillary blood sample volume and hematocrit to define peak (N) or nadir (T) time and concentration from individual daily measurements. Daily serum N peaked 2.50 ± 0.25 (SEM) ng/mL at a median (range) of 6 (4-13) days causing a reduction in serum T from 3.50 ± 0.57 ng/mL at baseline to a nadir of 0.38 ± 0.13 (SEM) ng/mL (89 ± 3% suppression) at a median (range) of 8 (5-16) days. Simultaneously sampled capillary, venous whole blood, and serum gave almost identical results for serum T and N. Finger-pricks and sc injections were well tolerated. This study demonstrates that A) DBS sampling with liquid chromatography mass spectrometry steroid analysis achieves frequent time sampling in the community without requiring clinic visits, venesection, or frozen serum storage, and B) androgen esters in an oil vehicle can be delivered effectively by sc injection, thus avoiding the need for medically supervised deep-im injections.

Design, fabrication and experimental validation of a novel dry-contact sensor for measuring electroencephalography signals without skin preparation.

PubMed

Liao, Lun-De; Wang, I-Jan; Chen, Sheng-Fu; Chang, Jyh-Yeong; Lin, Chin-Teng

2011-01-01

In the present study, novel dry-contact sensors for measuring electro-encephalography (EEG) signals without any skin preparation are designed, fabricated by an injection molding manufacturing process and experimentally validated. Conventional wet electrodes are commonly used to measure EEG signals; they provide excellent EEG signals subject to proper skin preparation and conductive gel application. However, a series of skin preparation procedures for applying the wet electrodes is always required and usually creates trouble for users. To overcome these drawbacks, novel dry-contact EEG sensors were proposed for potential operation in the presence or absence of hair and without any skin preparation or conductive gel usage. The dry EEG sensors were designed to contact the scalp surface with 17 spring contact probes. Each probe was designed to include a probe head, plunger, spring, and barrel. The 17 probes were inserted into a flexible substrate using a one-time forming process via an established injection molding procedure. With these 17 spring contact probes, the flexible substrate allows for high geometric conformity between the sensor and the irregular scalp surface to maintain low skin-sensor interface impedance. Additionally, the flexible substrate also initiates a sensor buffer effect, eliminating pain when force is applied. The proposed dry EEG sensor was reliable in measuring EEG signals without any skin preparation or conductive gel usage, as compared with the conventional wet electrodes.

Design, Fabrication and Experimental Validation of a Novel Dry-Contact Sensor for Measuring Electroencephalography Signals without Skin Preparation

PubMed Central

Liao, Lun-De; Wang, I-Jan; Chen, Sheng-Fu; Chang, Jyh-Yeong; Lin, Chin-Teng

2011-01-01

In the present study, novel dry-contact sensors for measuring electro-encephalography (EEG) signals without any skin preparation are designed, fabricated by an injection molding manufacturing process and experimentally validated. Conventional wet electrodes are commonly used to measure EEG signals; they provide excellent EEG signals subject to proper skin preparation and conductive gel application. However, a series of skin preparation procedures for applying the wet electrodes is always required and usually creates trouble for users. To overcome these drawbacks, novel dry-contact EEG sensors were proposed for potential operation in the presence or absence of hair and without any skin preparation or conductive gel usage. The dry EEG sensors were designed to contact the scalp surface with 17 spring contact probes. Each probe was designed to include a probe head, plunger, spring, and barrel. The 17 probes were inserted into a flexible substrate using a one-time forming process via an established injection molding procedure. With these 17 spring contact probes, the flexible substrate allows for high geometric conformity between the sensor and the irregular scalp surface to maintain low skin-sensor interface impedance. Additionally, the flexible substrate also initiates a sensor buffer effect, eliminating pain when force is applied. The proposed dry EEG sensor was reliable in measuring EEG signals without any skin preparation or conductive gel usage, as compared with the conventional wet electrodes. PMID:22163929

Role of poly-(ADP-ribose) synthetase in lipopolysaccharide-induced vascular failure and acute lung injury in pigs.

PubMed

Albertini, M; Clement, M G; Lafortuna, C L; Caniatti, M; Magder, S; Abdulmalek, K; Hussain, S N

2000-06-01

To assess the contribution of poly (adenosine 5'-diphosphate ribose) synthetase (PARS) to the development of bacterial lipopolysaccharide (LPS)-induced acute lung injury and vascular failure in pigs. Four groups of anesthetized, paralyzed, and mechanically ventilated domestic white pigs. Group 1 served as control, whereas Escherichia coli LPS (20 microg/kg/h) was continuously infused in group 2. Group 3 received 20 mg/kg injection of 3-aminobenzamide (a selective inhibitor of PARS activity) 15 minutes before LPS infusion. Only 3-aminobenzamide and not LPS was injected in group 4. All animals were examined for 180 minutes. Systemic and pulmonary hemodynamics and lung mechanics were measured during the experimental period. Lung wet/dry ratio, bronchoalveolar lavage (BAL) protein levels and cell counts and lung nitrotyrosine (footprint of peroxynitrite) immunostaining were also measured in a few animals. LPS infusion evoked a progressive decline in systemic arterial pressure, a small increase in cardiac output, and biphasic elevation of pulmonary arterial pressure. Lung compliance declined progressively, whereas lung and total respiratory resistance rose significantly after LPS infusion. Prominent nitrotyrosine immunostaining was detected around small airways and pulmonary endothelium of LPS-infused animals. No significant changes in lung wet/dry ratio and BAL protein levels and cell counts were produced by LPS infusion. Pretreatment with 3-aminobenzamide did not alter the systemic and pulmonary hemodynamic responses to LPS infusion but eliminated the rise in pulmonary and total respiratory resistance. We concluded that PARS activation plays an important role in the changes of lung mechanics associated with LPS-induced acute lung injury but had no role in vascular failure.

Liquid-type Botulinum Toxin Type A in Adductor Spasmodic Dysphonia: A Prospective Pilot Study.

PubMed

Cha, Wonjae; Jang, Jeon Yeob; Wang, Soo-Geun; Kang, Ji-Heon; Jo, Min-Gyu

2017-05-01

Botulinum toxin (BTX) has been widely used to treat adductor spasmodic dysphonia (ADSD). Most commercially available forms of BTX require reconstitution before use, which may increase the risk of contamination and requires careful titration. Recently, a liquid-type BTX type A (BTX-A) has been developed, which should simplify the procedure and enhance its efficacy. Herein, we present a prospective pilot study to investigate the efficacy and safety of liquid-type BTX-A in the treatment of ADSD. Twenty-six consecutive liquid-type BTX-A injections were performed in 12 patients with ADSD. We included as a control group 34 consecutive patients with ADSD who had previously undergone 52 vocal fold injection procedures with freeze-dried-type BTX-A. All patients in both groups had improvement of symptoms related to ADSD and period of normal voice. Most patients experienced breathiness, and the onset time, the peak response time, and the duration of breathiness were similar in both groups. The duration of effect (days) was 96.96 ± 18.91 and 77.38 ± 18.97 in the freeze-dried-type and the liquid-type groups, and the duration of benefit (days) was 80.02 ± 18.24 and 62.69 ± 19.73 in the freeze-dried-type and the liquid-type groups. To compare the efficacy between the freeze-dried-type and the liquid-type BTX-A, the sessions of the unilateral vocal fold injection were included and were categorized as group A (1 ~ 2 units BTX-A) and group B (2 ~ 3 units BTX-A), according to the dose per vocal fold. There was no significant difference of effect time between freeze-dried-type and liquid-type BTX-A groups. No adverse events related to BTX or vocal fold injection were reported. Liquid-type BTX-A is safe and effective for the treatment of spasmodic dysphonia. With the advantages of simple preparation, storage, and reuse and animal protein-free constituents, liquid-type BTX-A may be a good option in the treatment of spasmodic dysphonia. Copyright © 2017 The Voice Foundation. Published by Elsevier Inc. All rights reserved.

Monitoring an EGS injection at Newberry Volcano using Magnetotelluric dimensionality analysis

NASA Astrophysics Data System (ADS)

Bowles-martinez, E.; Schultz, A.; Rose, K.; Urquhart, S.

2016-12-01

The sensitivity of magnetotelluric (MT) data to the presence of electrically conductive subsurface features makes it applicable for determining the extent of injected fluids in enhanced geothermal systems (EGS). We use MT to monitor fluid injection during tests of a proposed EGS site at Newberry Volcano in Central Oregon, USA. Newberry is a large shield volcano located where fault systems of the northern Basin and Range meet the Cascade Arc and the high lava plains. Its strong potential for geothermal energy has made it a target for energy exploration for over 40 years. MT measurements were made before, during, and after an EGS stimulation in 2014 in an effort to detect subsurface pathways taken by fluids that are attributable to stimulation. We begin by creating a baseline model from inverting over 200 wideband MT stations located in the western half of the volcano. This model is constrained by well logs, as well as by high resolution gravity and seismic velocity modeling. Our model shows conductive regions associated with the caldera's ring fault, likely showing where hydrothermal fluids or their mineral alteration products are present. However, as this is an EGS study, we are interested in detecting fluid intrusion into hot, dry rock. Therefore, our primary target is a resistive zone on the western flank of Newberry volcano that is interpreted as a series of hot intrusive sequences. Well bottom temperatures in this area have been measured in excess of 300 °C. The stimulation's effect on resistivity is subtle, in part because the injected fluid is fresh groundwater, the injected volume is modest, and the target depth is 2,000-3,000 m below ground level. We found that it is advantageous to look at the impedance tensor data directly to detect injected fluids. Because fluids and their associated change in resistivity are expected to be concentrated around the injection well, the injection will exhibit a highly three-dimensional resistivity structure. Therefore, we examine the impedance tensor for changes in dimensionality to mark the arrival of injected fluids. We then present a method of inverting MT data for changes in impedance rather than for resistivity.

The mammalian response to lunar particulates.

NASA Technical Reports Server (NTRS)

Holland, J. M.; Simmonds, R. C.

1973-01-01

The response of germfree mice to subcutaneous and intraperitoneal injection of aqueous suspensions of lunar fine material (LFM) was evaluated. Both uninjected mice and mice injected with dry heat sterilized LFM were included as controls. After injection, the majority of mice were subjected to serial sacrifice to assess the time course of the tissue response. A smaller group of animals were held for lifespan studies. The observations suggest that LFM is relatively insoluble in tissue and that, while acting as a low grade irritant, it has little tendency to evoke reactive fibrosis.

Ion channel recordings on an injection-molded polymer chip.

PubMed

Tanzi, Simone; Matteucci, Marco; Christiansen, Thomas Lehrmann; Friis, Søren; Christensen, Mette Thylstrup; Garnaes, Joergen; Wilson, Sandra; Kutchinsky, Jonatan; Taboryski, Rafael

2013-12-21

In this paper, we demonstrate recordings of the ion channel activity across the cell membrane in a biological cell by employing the so-called patch clamping technique on an injection-molded polymer microfluidic device. The findings will allow direct recordings of ion channel activity to be made using the cheapest materials and production platform to date and with the potential for very high throughput. The employment of cornered apertures for cell capture allowed the fabrication of devices without through holes and via a scheme comprising master origination by dry etching in a silicon substrate, electroplating in nickel and injection molding of the final part. The most critical device parameters were identified as the length of the patching capillary and the very low surface roughness on the inside of the capillary. The cross-sectional shape of the orifice was found to be less critical, as both rectangular and semicircular profiles seemed to have almost the same ability to form tight seals with cells with negligible leak currents. The devices were functionally tested using human embryonic kidney cells expressing voltage-gated sodium channels (Nav1.7) and benchmarked against a commercial state-of-the-art system for automated ion channel recordings. These experiments considered current-voltage (IV) relationships for activation and inactivation of the Nav1.7 channels and their sensitivity to a local anesthetic, lidocaine. Both IVs and lidocaine dose-response curves obtained from the injection-molded polymer device were in good agreement with data obtained from the commercial system.

VACCINATION AGAINST YELLOW FEVER WITH IMMUNE SERUM AND VIRUS FIXED FOR MICE

PubMed Central

Sawyer, W. A.; Kitchen, S. F.; Lloyd, Wray

1932-01-01

1. After preliminary experiments in monkeys, 15 persons were actively immunized by a single injection of a dried mixture of living yellow fever virus, fixed for mice, and human immune serum, with separate injections of enough additional serum to make up the amount required for protection. 2. One person was similarly immunized by injecting immune serum and dried virus separately. 3. By titration of the sera of vaccinated persons in mice, it was shown that the immunity rose in a few weeks to a height comparable to that reached after an attack of yellow fever, and remained there throughout an observation period of 6 months. 4. Yellow fever virus could not be recovered from the blood of vaccinated persons or monkeys, except when the latter had received less than the minimal effective amount of immune serum. 5. Neutralization of yellow fever virus by immune serum took place very slowly in vitro at room temperature in our experiments, and could not have been an appreciable factor in vaccination with the serum virus mixtures. 6. A mixture of fixed virus and immune serum retained its immunizing power for 8 months when dried in the frozen state and sealed in glass. 7. It appears that the immunizing reaction after yellow fever vaccination was a part of a true infectious process, as was also the observed leucopenia. PMID:19870044

Thrust shock vector control of an axisymmetric conical supersonic nozzle via secondary transverse gas injection

NASA Astrophysics Data System (ADS)

Zmijanovic, V.; Lago, V.; Sellam, M.; Chpoun, A.

2014-01-01

Transverse secondary gas injection into the supersonic flow of an axisymmetric convergent-divergent nozzle is investigated to describe the effects of the fluidic thrust vectoring within the framework of a small satellite launcher. Cold-flow dry-air experiments are performed in a supersonic wind tunnel using two identical supersonic conical nozzles with the different transverse injection port positions. The complex three-dimensional flow field generated by the supersonic cross-flows in these test nozzles was examined. Valuable experimental data were confronted and compared with the results obtained from the numerical simulations. Different nozzle models are numerically simulated under experimental conditions and then further investigated to determine which parameters significantly affect thrust vectoring. Effects which characterize the nozzle and thrust vectoring performances are established. The results indicate that with moderate secondary to primary mass flow rate ratios, ranging around 5 %, it is possible to achieve pertinent vector side forces. It is also revealed that injector positioning and geometry have a strong effect on the shock vector control system and nozzle performances.

Mechanical Weakening during Fluid Injection in Critically Stressed Sandstones with Acoustic Monitoring

NASA Astrophysics Data System (ADS)

David, C.; Dautriat, J. D.; Sarout, J.; Macault, R.; Bertauld, D.

2014-12-01

Water weakening is a well-known phenomenon which can lead to subsidence during the production of hydrocarbon reservoirs. The example of the Ekofisk oil field in the North Sea has been well documented for years. In order to assess water weakening effects in reservoir rocks, previous studies have focused on changes in the failure envelopes derived from mechanical tests conducted on rocks saturated either with water or with inert fluids. However, little attention has been paid so far on the mechanical behaviour during the fluid injection stage, like in enhanced oil recovery operations. We studied the effect of fluid injection on the mechanical behaviour of Sherwood sandstone, a weakly-consolidated sandstone sampled at Ladram Bay in UK. In order to highlight possible weakening effects, water and inert oil have been injected into critically-loaded samples to assess their effect on strength and elastic properties and to derive the acoustic signature of the saturation front for each fluid. The specimens were instrumented with 16 ultrasonic P-wave transducers for both passive and active acoustic monitoring during fluid injection and loading. After conducting standard triaxial tests on three samples saturated with air, water and oil respectively, mechanical creep tests were conducted on dry samples loaded at 80% of the compressive strength of the dry rock. While these conditions are kept constant, a fluid is injected at the bottom end of the sample with a low back pressure (0.5 MPa) to minimize effective stress variations during injection. Both water and oil were used as the injected pore fluid in two experiments. As soon as the fluids start to flow into the samples, creep is taking place with a much higher strain rate for water injection compared to oil injection. A transition from secondary creep to tertiary creep is observed in the water injection test whereas in the oil injection test no significant creep acceleration is observed after one pore volume of oil was injected. The most remarkable difference is that water injection induces mechanical instability and failure, whereas oil injection does not. This was confirmed by the analysis of acoustic emissions activity and post-mortem sample imaging using CT scan. Contrasting evolutions of the P wave velocity during the fluid front propagation were also observed in both experiments.

Retention of Structure and Function of the Cat Germinal Vesicle after Air-Drying and Storage at Suprazero Temperature1

PubMed Central

Graves-Herring, Jennifer E.; Wildt, David E.; Comizzoli, Pierre

2013-01-01

ABSTRACT The study explored a novel approach for preserving the maternal genome without the entire oocyte by air-drying the cat germinal vesicle (GV) in the presence of the disaccharide trehalose. Specifically, we examined GV structure and function after desiccation, storage at 4°C (up to 32 wk), and rehydration including the ability to resume meiosis after injection into a fresh, conspecific cytoplast. In experiment 1, DNA integrity was similar to fresh controls after 1 and 4 wk storage in the presence of trehalose, but was more fragmented at later time points (especially after 32 wk). Nuclear envelope integrity was sustained in >90% of oocytes stored for 0, 4, or 16 wk regardless of protective treatment. In experiment 2, compacted, air-dried GVs were stored for 2 or 4 wk, rehydrated, and injected into fresh cytoplasts. After culture for 24 h in vitro, up to 73% of oocytes reconstructed with desiccated GVs preserved in trehalose resumed meiosis compared to 30% of those dried in the absence of the disaccharide. At each storage time point, trehalose presence during air-drying was advantageous for resumption of meiosis, with >20% of oocytes completing nuclear maturation to metaphase II. This demonstrates a potential for preserving the female genome using the GV alone and for multiple weeks after desiccation. Trehalose enhanced the process by retaining the ability of a dried and rehydrated GV to resume communication with the surrounding cytoplasm of the recipient oocyte to permit reaching metaphase II and likely sustain subsequent embryo development. PMID:23575153

Three-dimensional numerical reservoir simulation of the EGS Demonstration Project at The Geysers geothermal field

NASA Astrophysics Data System (ADS)

Borgia, Andrea; Rutqvist, Jonny; Oldenburg, Curt M.; Hutchings, Lawrence; Garcia, Julio; Walters, Mark; Hartline, Craig; Jeanne, Pierre; Dobson, Patrick; Boyle, Katie

2013-04-01

The Enhanced Geothermal System (EGS) Demonstration Project, currently underway at the Northwest Geysers, California, aims to demonstrate the feasibility of stimulating a deep high-temperature reservoir (up to 400 °C) through water injection over a 2-year period. On October 6, 2011, injection of 25 l/s started from the Prati 32 well at a depth interval of 1850-2699 m below sea level. After a period of almost 2 months, the injection rate was raised to 63 l/s. The flow rate was then decreased to 44 l/s after an additional 3.5 months and maintained at 25 l/s up to August 20, 2012. Significant well-head pressure changes were recorded at Prati State 31 well, which is separated from Prati 32 by about 500 m at reservoir level. More subdued pressure increases occur at greater distances. The water injection caused induced seismicity in the reservoir in the vicinity of the well. Microseismic monitoring and interpretation shows that the cloud of seismic events is mainly located in the granitic intrusion below the injection zone, forming a cluster elongated SSE-NNW (azimuth 170°) that dips steeply to the west. In general, the magnitude of the events increases with depth and the hypocenter depth increases with time. This seismic cloud is hypothesized to correlate with enhanced permeability in the high-temperature reservoir and its variation with time. Based on the existing borehole data, we use the GMS™ GUI to construct a realistic three-dimensional (3D) geologic model of the Northwest Geysers geothermal field. This model includes, from the top down, a low permeability graywacke layer that forms the caprock for the reservoir, an isothermal steam zone (known as the normal temperature reservoir) within metagraywacke, a hornfels zone (where the high-temperature reservoir is located), and a felsite layer that is assumed to extend downward to the magmatic heat source. We then map this model onto a rectangular grid for use with the TOUGH2 multiphase, multicomponent, non-isothermal porous media numerical flow simulator in order to model the evolution and injection-related operational dynamics of The Geysers geothermal field. At the bottom of the domain in the felsite, we impose a constant temperature, constant saturation, low-permeability boundary. Laterally we set no-flow boundaries (no mass or heat flow), while at the top we use a fully aqueous-phase-saturated constant atmospheric pressure boundary condition. We compute initial conditions for two different conceptual models. The first conceptual model has two phases (gas and aqueous) with decreasing proportions of gas from the steam zone downward; the second model has dry steam all the way from the steam zone to the bottom. The first may be more similar to a pre-exploitation condition, before production reduced pressure and dried out the system, while the second is calibrated to the pressure and temperature actually measured in the reservoir today. Our preliminary results are in reasonable agreement with the pressure monitoring at Prati State 31. These results will be used in hydrogeomechanical modeling to plan, design, and validate the effects of injection in the system.

Pneumatic testing in 45-degree-inclined boreholes in ash-flow tuff near Superior, Arizona

USGS Publications Warehouse

LeCain, G.D.

1995-01-01

Matrix permeability values determined by single-hole pneumatic testing in nonfractured ash-flow tuff ranged from 5.1 to 20.3 * 1046 m2 (meters squared), depending on the gas-injection rate and analysis method used. Results from the single-hole tests showed several significant correlations between permeability and injection rate and between permeability and test order. Fracture permeability values determined by cross-hole pneumatic testing in fractured ash-flow tuff ranged from 0.81 to 3.49 * 1044 m2, depending on injection rate and analysis method used. Results from the cross-hole tests monitor intervals showed no significant correlation between permeability and injection rate; however, results from the injection interval showed a significant correlation between injection rate and permeability. Porosity estimates from the 'cross-hole testing range from 0.8 to 2.0 percent. The maximum temperature change associated with the pneumatic testing was 1.2'(2 measured in the injection interval during cross-hole testing. The maximum temperature change in the guard and monitor intervals was O.Ip C. The maximum error introduced into the permeability values due to temperature fluctuations is approximately 4 percent. Data from temperature monitoring in the borehole indicated a positive correlation between the temperature decrease in the injection interval during recovery testing and the gas-injection rate. The thermocouple psychrometers indicated that water vapor was condensing in the boreholes during testing. The psychrometers in the guard and monitor intervals detected the drier injected gas as an increase in the dry bulb reading. The relative humidity in the test intervals was always higher than the upper measurement limit of the psychrometers. Although the installation of the packer system may have altered the water balance of the borehole, the gas-injection testing resulted in minimal or no changes in the borehole relative humidity.

Sperm preservation by freeze-drying for the conservation of wild animals.

PubMed

Kaneko, Takehito; Ito, Hideyuki; Sakamoto, Hidefusa; Onuma, Manabu; Inoue-Murayama, Miho

2014-01-01

Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4 °C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the storage and transportation of sperm. We demonstrate that chimpanzee, giraffe, jaguar, weasel and the long-haired rat sperm remain viable after freeze-drying. In all species, pronuclei were formed after the injection of freeze-dried sperm into the mouse oocytes. Although preliminary, these results may be useful for the future establishment of "freeze-drying zoo" to conserve wild animals.

Environmental & economic life cycle assessment of current & future sewage sludge to energy technologies.

PubMed

Mills, N; Pearce, P; Farrow, J; Thorpe, R B; Kirkby, N F

2014-01-01

The UK Water Industry currently generates approximately 800GWh pa of electrical energy from sewage sludge. Traditionally energy recovery from sewage sludge features Anaerobic Digestion (AD) with biogas utilisation in combined heat and power (CHP) systems. However, the industry is evolving and a number of developments that extract more energy from sludge are either being implemented or are nearing full scale demonstration. This study compared five technology configurations: 1 - conventional AD with CHP, 2 - Thermal Hydrolysis Process (THP) AD with CHP, 3 - THP AD with bio-methane grid injection, 4 - THP AD with CHP followed by drying of digested sludge for solid fuel production, 5 - THP AD followed by drying, pyrolysis of the digested sludge and use of the both the biogas and the pyrolysis gas in a CHP. The economic and environmental Life Cycle Assessment (LCA) found that both the post AD drying options performed well but the option used to create a solid fuel to displace coal (configuration 4) was the most sustainable solution economically and environmentally, closely followed by the pyrolysis configuration (5). Application of THP improves the financial and environmental performance compared with conventional AD. Producing bio-methane for grid injection (configuration 3) is attractive financially but has the worst environmental impact of all the scenarios, suggesting that the current UK financial incentive policy for bio-methane is not driving best environmental practice. It is clear that new and improving processes and technologies are enabling significant opportunities for further energy recovery from sludge; LCA provides tools for determining the best overall options for particular situations and allows innovation resources and investment to be focused accordingly. Copyright © 2013 The Authors. Published by Elsevier Ltd.. All rights reserved.

Flow monitoring of microwave pre-heated resin in LCM processes

NASA Astrophysics Data System (ADS)

Rubino, F.; Paradiso, V.; Carlone, P.

2017-10-01

Liquid composite molding is manufacturing techniques that involve the injection or infusion of catalyzed liquid resin into a mold to impregnate a dry fiber preform. The challenges of LCM processes are related to the obtaining of a complete wetting of the reinforcement as well as a reduction of the void to obtain a final product with high mechanical properties. The heating of the resin prior the injection into the mold cavity has proven to be useful to improve the LCM processes. The increasing of temperature results in a reduction of resin viscosity and allows the resin to flow more easily through the reinforcement; the cure stage is also improved resulting in a reduction of global process time required. Besides the conventional solutions to heat up the resin based on the thermal conduction, in-line microwave heating is a suitable method to heat dielectric materials providing an even temperature distribution through the resin, thereby avoiding a thermal gradient between the surface and the core of liquid resin, which could result in a premature and uncontrolled cure. In the present work, an in-line microwave system, manually controlled, have been coupled with a VARTM apparatus to heat the resin before the infusion. In addition, parallel-plate dielectric sensors and pressure sensors, embedded into the mold, were employed to track the flow front through the fiber reinforcement in two distinct cases: unheated resin and pre-heated resin. The aim of work was to assess the effectiveness of microwave pre-heating to improve the macro and micro-impregnation of dry preform. The obtained results showed capability of in-line microwave heating to shorten the impregnation of dry fabric and provide a homogeneous wetting of fibers.

Process and apparatus for indirect-fired heating and drying

DOEpatents

Abbasi, Hamid Ali; Chudnovsky, Yaroslav

2005-04-12

A method for heating flat or curved surfaces comprising injecting fuel and oxidant along the length, width or longitudinal side of a combustion space formed between two flat or curved plates, transferring heat from the combustion products via convection and radiation to the surface being heated on to the material being dried/heated, and recirculating at least 20% of the combustion products to the root of the flame.

Development and Characterization of Sodium Hyaluronate Microparticle-Based Sustained Release Formulation of Recombinant Human Growth Hormone Prepared by Spray-Drying.

PubMed

Kim, Sun J; Kim, Chan W

2016-02-01

The purpose of this study was to develop and characterize a sodium hyaluronate microparticle-based sustained release formulation of recombinant human growth hormone (SR-rhGH) prepared by spray-drying. Compared to freeze-drying, spray-dried SR-rhGH showed not only prolonged release profiles but also better particle property and injectability. The results of size-exclusion high-performance liquid chromatography showed that no aggregate was detected, and dimer was just about 2% and also did not increase with increase of inlet temperature up to 150 °C. Meanwhile, the results of reversed-phase high-performance liquid chromatography revealed that related proteins increased slightly from 4.6% at 100 °C to 6.3% at 150 °C. Thermal mapping test proved that product temperature did not become high to cause protein degradation during spray-drying because thermal energy was used for the evaporation of surface moisture of droplets. The structural characterization by peptide mapping, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and circular dichroism revealed that the primary, secondary, and tertiary structures of rhGH in SR-rhGH were highly comparable to those of reference somatropin materials. The biological characterization by rat weight gain and cell proliferation assays provided that bioactivity of SR-rhGH was equivalent to that of native hGH. These data establish that spray-dried SR-rhGH is highly stable by preserving intact rhGH and hyaluronate microparticle-based formulation by spray-drying can be an alternative delivery system for proteins. Copyright © 2016 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.

Gras Dowr joins world`s FPSO fleet

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1997-05-05

The Gras Dowr, a floating production, storage, and offloading vessel (FPSD) for Amerada Hess Ltd.`s North Sea Durward and Dauntless fields, is one of the latest additions to the world`s growing FPSO fleet. The Gras Dowr, anchored in about 90 m of water, lies between the Durward (U.K. Block 21/16) and Dauntless (U.K. Block 21/11) fields, about 3.5 km from the subsea wellhead locations. The Gras Dowr`s main functions, according to Bluewater Offshore Production Systems Ltd., are to: receive fluids from well risers; process incoming fluids to separate the fluid into crude, water, and gas; store dry crude oil andmore » maintain the required temperature; treat effluent to allow for water discharge to the sea; compress gas for gas lift as a future option; provide chemical injection skid for process chemical injection; use a part of the produced gas for fuel gas, and flare excess gas; inject treated seawater into the injection wells; house power generation for process and offloading operation and utilities; offload to a tandem moored shuttle tanker including receiving liquid fuel from the same tanker; provide accommodations for operating and maintenance crews; allow helicopters landings and takeoffs; allow handling and storage of goods transported by supply vessels; moor a shuttle tanker; and control the subsea wells.« less

Clove essential oil-in-cyclodextrin-in-liposomes in the aqueous and lyophilized states: From laboratory to large scale using a membrane contactor.

PubMed

Sebaaly, Carine; Charcosset, Catherine; Stainmesse, Serge; Fessi, Hatem; Greige-Gerges, Hélène

2016-03-15

This work is dedicated to prepare liposomal dry powder formulations of inclusion complexes of clove essential oil (CEO) and its main component eugenol (Eug). Ethanol injection method and membrane contactor were applied to prepare liposomes at laboratory and large scale, respectively. Various liposomal formulations were tested: (1) free hydroxypropyl-β-cyclodextrin loaded liposomes; (2) drug in hydroxypropyl-β-cyclodextrin in liposomes (DCL); (3) DCL2 obtained by double loading technique, where the drug is added in the organic phase and the inclusion complex in the aqueous phase. Liposomes were characterized for their particle size, polydispersity index, Zeta potential, morphology, encapsulation efficiency of CEO components and Eug loading rate. Reproducible results were obtained with both injection devices. Compared to Eug-loaded liposomes, DCL and DCL2 improved the loading rate of Eug and possessed smaller vesicles size. The DPPH(•) scavenging activity of Eug and CEO was maintained upon incorporation of Eug and CEO into DCL and DCL2. Contrary to DCL2, DCL formulations were stable after 1 month of storage at 4°C and upon reconstitution of the dried lyophilized cakes. Hence, DCL in aqueous and lyophilized forms, are considered as a promising carrier system to preserve volatile and hydrophobic drugs enlarging their application in cosmetic, pharmaceutical and food industries. Copyright © 2015 Elsevier Ltd. All rights reserved.

Controlling a hurricane by altering its internal climate

NASA Astrophysics Data System (ADS)

Mardhekar, D.

2010-09-01

Atmospheric hazards, like the fury of a hurricane, can be controlled by altering its internal climate. The hurricane controlling technique suggested is eco-friendly, compatible with hurricane size, has a sound scientific base and is practically possible. The key factor is a large scale dilution of the hurricane fuel, vapour, in the eye wall and spiral rain bands where condensation causing vapor volume reduction (a new concept which can be explained by Avogadro's law) and latent heat release drive the storm. This can be achieved by installing multiple storage tanks containing dry liquefied air on the onshore and offshore coastal regions and islands, preferably underground, in the usual path of a hurricane. Each storage tank is designed to hold and release dry liquefied air of around 100,000 tons. Satellite tracking of hurricanes can locate the eye wall and the spiral rain bands. The installed storage tanks coming under these areas will rapidly inject dry air in huge quantities thereby diluting the vapour content of the vapour-rich air in the eye wall and in the spiral rain bands. This will result in reduced natural input of vapour-rich air, reduced release of latent heat, reduced formation of the low pressure zone due to condensation and volume reduction of the vapor, expansion of the artificially introduced dry air as it goes up occupying a larger space with the diluted fuel, absorption of energy from the system by low temperature of the artificially introduced air. It will effect considerable condensation of the vapor near the sea surface thus further starving the hurricane of its fuel in its engine. Seeding materials, or microscopic dust as suggested by Dr. Daniel Rosenfeld in large quantities may also be introduced via the flow of the injected dry air in order to enhance the hurricane controlling ability. All the above factors are in favour of retarding the hurricane's wind speed and power. The sudden weakening of hurricane Lili was found to be partially caused by the natural input of drier air. We are artificially introducing completely dry air in large quantities (perhaps the dry air release is from 100,000 tons X 50 tanks or 100 tanks or even more tanks, whatever comes under the defined areas) and that too along with seeding materials if required. Importantly, these mega introductions are directly into the proper areas of a hurricane. Optionally, the dry air introduction may be done anywhere in the core of a hurricane. We can even tame a hurricane in a controlled manner by controlling the volumes of the release of the dry gas, the seeding materials and the locations of the release so that fresh water in the form of rains can come on land simultaneously limiting the destruction. Most importantly the dissipation of the hurricanes hitting the coastal regions can be effectively accelerated to control the destruction by introducing the dry air from the coastal region tanks. Here the land effect and simultaneous introduction of the dry air will give a synergic effect.

Sperm Preservation by Freeze-Drying for the Conservation of Wild Animals

PubMed Central

Kaneko, Takehito; Ito, Hideyuki; Sakamoto, Hidefusa; Onuma, Manabu; Inoue-Murayama, Miho

2014-01-01

Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4°C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the storage and transportation of sperm. We demonstrate that chimpanzee, giraffe, jaguar, weasel and the long-haired rat sperm remain viable after freeze-drying. In all species, pronuclei were formed after the injection of freeze-dried sperm into the mouse oocytes. Although preliminary, these results may be useful for the future establishment of “freeze-drying zoo” to conserve wild animals. PMID:25409172

Apparatus for control of mercury

DOEpatents

Downs, William; Bailey, Ralph T.

2001-01-01

A method and apparatus for reducing mercury in industrial gases such as the flue gas produced by the combustion of fossil fuels such as coal adds hydrogen sulfide to the flue gas in or just before a scrubber of the industrial process which contains the wet scrubber. The method and apparatus of the present invention is applicable to installations employing either wet or dry scrubber flue gas desulfurization systems. The present invention uses kraft green liquor as a source for hydrogen sulfide and/or the injection of mineral acids into the green liquor to release vaporous hydrogen sulfide in order to form mercury sulfide solids.

Soil Modification by Native Shrubs Boosts Crop Productivity in Sudano-Sahelian Agroforestry System

NASA Astrophysics Data System (ADS)

Bogie, N. A.; Bayala, R.; Diedhiou, I.; Ghezzehei, T. A.; Dick, R.

2014-12-01

A changing climate along with human and animal population pressure can have a devastating effect on crop yields and food security in the Sudano-Sahel. Agricultural solutions to address soil degradation and crop water stress are needed to combat this increasingly difficult situation. Significant differences in crop success have been observed in peanut and millet grown in association with two native evergreen shrubs Piliostigma reticulatum, and Guiera senegalensis at the sites of Nioro du Rip and Keur Matar, respectively.We investigate how farmers can increase crop productivity by capitalizing on the evolutionary adaptation of native shrubs to the harsh Sudano-Sahelian environment as well as the physical mechanisms at work in the system that can lead to more robust yields. Soil moisture and water potential data were collected during a dry season millet irrigation experiment where stress was imposed in the intercropped system. Despite lower soil moisture content, crops grown in association with shrubs have increased biomass production and a faster development cycle. Hydraulic redistribution is thought to exist in this system and we found diurnal fluctuations in water potential within the intercropped system that increased in magnitude of to 0.4 Mpa per day as the soil dried below 1.0 Mpa during the stress treatment. An isotopic tracer study investigating hydraulic redistribution was carried out by injecting labeled water into shrub roots and sampling shrubs and nearby crops for isotopic analysis of plant water. These findings build on work that was completed in 2004 at the site, but point to lower overall magnitude of diurnal soil water potential fluctuations in dry soils. Using even the limited resources that farmers possess, this agroforestry technique can be expanded over wide swaths of the Sahel.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sherman, G.J.; Schuett, K.J.; White, D.G.

Number 13 Blast Furnace at Gary began injecting Pulverized Coal in March 1993. The injection level was increased over the next nine months until a level off 409 lbs/THM was achieved for the month of December 1993. Several major areas were critical in achieving this high level of Pulverized coal injection (PCI) including furnace conditions, lance position, tuyere blockage, operating philosophy, and outages. The paper discusses the modifications made to achieve this level of injection. This injection level decreased charged dry coke rate from 750 lbs/THM to about 625 lbs/THM, while eliminating 150 lbs/THM of oil and 20 lbs/THM ofmore » natural gas. Assuming a 1.3 replacement ratio for an oil/natural gas mixture, overall coke replacement for the coal is about 0.87 lbs coke/lbs coal. Gary Works anticipates levels of 500 lbs/THM are conceivable.« less

Human Growth Hormone Delivery with a Microneedle Transdermal System: Preclinical Formulation, Stability, Delivery and PK of Therapeutically Relevant Doses

PubMed Central

Ameri, Mahmoud; Kadkhodayan, Miryam; Nguyen, Joe; Bravo, Joseph A.; Su, Rebeca; Chan, Kenneth; Samiee, Ahmad; Daddona, Peter E.

2014-01-01

This study evaluated the feasibility of coating formulated recombinant human growth hormone (rhGH) on a titanium microneedle transdermal delivery system, Zosano Pharma (ZP)-hGH, and assessed preclinical patch delivery performance. Formulation rheology and surface activity were assessed by viscometry and contact angle measurement. rhGH liquid formulation was coated onto titanium microneedles by dip-coating and drying. The stability of coated rhGH was determined by size exclusion chromatography-high performance liquid chromatography (SEC-HPLC). Preclinical delivery and pharmacokinetic studies were conducted in female hairless guinea pigs (HGP) using rhGH coated microneedle patches at 0.5 and 1 mg doses and compared to Norditropin® a commercially approved rhGH subcutaneous injection. Studies demonstrated successful rhGH formulation development and coating on microneedle arrays. The ZP-hGH patches remained stable at 40 °C for six months with no significant change in % aggregates. Pharmacokinetic studies showed that the rhGH-coated microneedle patches, delivered with high efficiency and the doses delivered indicated linearity with average Tmax of 30 min. The absolute bioavailability of the microneedle rhGH patches was similar to subcutaneous Norditropin® injections. These results suggest that ZP-transdermal microneedle patch delivery of rhGH is feasible and may offer an effective and patient-friendly alternative to currently marketed rhGH injectables. PMID:24838219

Duct injection for SO{sub 2} control, Design Handbook, Volume 1, Process design and engineering guidelines

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

PETC developed a comprehensive program of coal-related, acid-rain research and development with a major activity area centering on flue gas cleanup and control of SO{sub 2} emissions. Particular emphasis was placed on the retrofit measures for older coal-fired power plants which predate the 1971 New Source Performance Standards. Candidate emission control technologies fall into three categories, depending upon their point of application along the fuel path (i.e., pre, during, or post combustion). The post-combustion, in-duct injection of a calcium-based chemical reagent seemed promising. Preliminary studies showed that reagent injection between the existing air heater and electrostatic precipitator (ESP) could removemore » between 50-60% of the SO{sub 2} and produce an environmentally safe, dry, solid waste that is easily disposed. Although SO{sub 2} removal efficiencies were less, the estimated capital costs for duct injection technology were low making the economics of duct injection systems seem favorable when compared to conventional wet slurry scrubbers under certain circumstances. With the promulgation of the Clean Air Act Amendments of 1990 came more incentive for the development of low capital cost flue gas desulfurization (FGD) processes. A number of technical problems had to be resolved, however, before duct injection technology could be brought to a state of commercial readiness. The Duct Injection Technology Development Program was launched as a comprehensive, four-year research effort undertaken by PETC to develop this new technology. Completed in 1992, this Duct Injection Design Handbook and the three-dimensional predictive mathematical model constitute two primary end products from this development program. The aim of this design handbook and the accompanying math model is to provide utility personnel with sufficient information to evaluate duct injection technology against competing SO{sub 2} emissions reduction strategies for an existing plant.« less

Industrial hazardous waste treatment featuring a rotary kiln and grate furnace incinerator: a case study in China.

PubMed

Ma, Pan; Ma, Zengyi; Yan, Jianhua; Chi, Yong; Ni, Mingjiang; Cen, Kefa

2011-10-01

As one of the fastest developing countries, China is facing severe problems concerning hazardous waste treatment and disposal. This paper presents a new incineration technology and demonstration project in eastern China. The incineration system includes a rotary kiln, a grate furnace for burning out the kiln residue and a flue gas post-combustion chamber. Flue gas treatment and emission control is based on: a quench tower, followed by dry hydrated lime and activated carbon injection, a dual bag filter system, and a wet scrubber. It demonstrated that this incineration technology can effectively dispose of industrial hazardous waste with variable and complex characteristics. Gas emissions meet the demands of the Chinese Environmental Protection Association standard.

Shrinkage of spray-freeze-dried microparticles of pure protein for ballistic injection by manipulation of freeze-drying cycle.

PubMed

Straller, Georg; Lee, Geoffrey

2017-10-30

Spray-freeze-drying was used to produce shrivelled, partially-collapsed microparticles of pure proteins that may be suitable for use in a ballistic injector. Various modifications of the freeze drying cycle were examined for their effects on collapse of the pure protein microparticles. The use of annealing at a shelf temperature of up to +10°C resulted in no visible particle shrinkage. This was because of the high T g ' of the pure protein. Inclusion of trehalose or sucrose led to particle shrinkage because of the plasticizing effects of the disaccharides on the protein. Only by extending the duration of primary drying from 240 to 2745min at shelf temperatures in the range -12 to -8°C were shrivelled, wrinkled particles of bSA and bCA of reduced porosity obtained. Manipulation of the freeze-drying cycle used for SFD can therefore be used to modify particle morphology and increase particle density. Copyright © 2017 Elsevier B.V. All rights reserved.

A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue.

PubMed

Hou, Aihua; Bose, Tanima; Chandy, K George; Tong, Louis

2017-06-07

Dry eye disease is a very common condition that causes morbidity and healthcare burden and decreases the quality of life. There is a need for a suitable dry eye animal model to test novel therapeutics to treat autoimmune dry eye conditions. This protocol describes a chronic autoimmune dry eye rat model. Lewis rats were immunized with an emulsion containing lacrimal gland extract, ovalbumin, and complete Freund's adjuvant. A second immunization with the same antigens in incomplete Freund's adjuvant was administered two weeks later. These immunizations were administered subcutaneously at the base of the tail. To boost the immune response at the ocular surface and lacrimal glands, lacrimal gland extract and ovalbumin were injected into the forniceal subconjunctiva and lacrimal glands 6 weeks after the first immunization. The rats developed dry eye features, including reduced tear production, decreased tear stability, and increased corneal damage. Immune profiling by flow cytometry showed a preponderance of CD3 + effector memory T cells in the eyeball.

A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

PubMed Central

Hou, Aihua; Bose, Tanima; Chandy, K. George; Tong, Louis

2017-01-01

Dry eye disease is a very common condition that causes morbidity and healthcare burden and decreases the quality of life. There is a need for a suitable dry eye animal model to test novel therapeutics to treat autoimmune dry eye conditions. This protocol describes a chronic autoimmune dry eye rat model. Lewis rats were immunized with an emulsion containing lacrimal gland extract, ovalbumin, and complete Freund's adjuvant. A second immunization with the same antigens in incomplete Freund's adjuvant was administered two weeks later. These immunizations were administered subcutaneously at the base of the tail. To boost the immune response at the ocular surface and lacrimal glands, lacrimal gland extract and ovalbumin were injected into the forniceal subconjunctiva and lacrimal glands 6 weeks after the first immunization. The rats developed dry eye features, including reduced tear production, decreased tear stability, and increased corneal damage. Immune profiling by flow cytometry showed a preponderance of CD3+ effector memory T cells in the eyeball. PMID:28654074

The Complex Physical-Chemical Interaction of Fracking Fluids with Gas Shale

NASA Astrophysics Data System (ADS)

Cathles, L. M.; Engelder, T.; Bryndzia, T.

2014-12-01

The chemical aspects of hydrofracturing might seem straight forward: Inject a fluid with sand and some chemicals, recover the injected water now contaminated with chemicals from the shale, and produce gas. But there are some complications that turn out to be very interesting. First of all, it is possible to recover only about 20% of the injected water. Secondly, the fresh injected water (1-5 kppm) has been turned into a very saline bine (~200 kppm). It's easy to say the water has just been imbibed into the gas-filled dry shale, like water into a dry sponge, except the organic parts of the shale which host nearly all the porosity are hydrophobic. The shale is strongly oil wet; nevertheless it imbibes water. It's easy to say the water just mixed with water in the shale and became salty, but there is almost no water in the shale, and no salt either. How the water becomes salty begs easy explanation. The talk will quantitatively discuss these issues in light of experiments we have carried out, concluding that powerful capillary and osmotic forces draw fracking water into the shale while making the return waters salty. How this is achieved will certainly tell us something about the fracture network and its connections. The practical implication is that hydrofracture fluids will be locked into the same "permeability jail" that sequestered overpressured gas for over 200 million years. If one wants to dispose of fracking waters, one could probably not choose a safer way to do so that to inject them into a gas shale.

Storage capacity in hot dry rock reservoirs

DOEpatents

Brown, D.W.

1997-11-11

A method is described for extracting thermal energy, in a cyclic manner, from geologic strata which may be termed hot dry rock. A reservoir comprised of hot fractured rock is established and water or other liquid is passed through the reservoir. The water is heated by the hot rock, recovered from the reservoir, cooled by extraction of heat by means of heat exchange apparatus on the surface, and then re-injected into the reservoir to be heated again. Water is added to the reservoir by means of an injection well and recovered from the reservoir by means of a production well. Water is continuously provided to the reservoir and continuously withdrawn from the reservoir at two different flow rates, a base rate and a peak rate. Increasing water flow from the base rate to the peak rate is accomplished by rapidly decreasing backpressure at the outlet of the production well in order to meet periodic needs for amounts of thermal energy greater than a baseload amount, such as to generate additional electric power to meet peak demands. The rate of flow of water provided to the hot dry rock reservoir is maintained at a value effective to prevent depletion of the liquid inventory of the reservoir. 4 figs.

Storage capacity in hot dry rock reservoirs

DOEpatents

Brown, Donald W.

1997-01-01

A method of extracting thermal energy, in a cyclic manner, from geologic strata which may be termed hot dry rock. A reservoir comprised of hot fractured rock is established and water or other liquid is passed through the reservoir. The water is heated by the hot rock, recovered from the reservoir, cooled by extraction of heat by means of heat exchange apparatus on the surface, and then re-injected into the reservoir to be heated again. Water is added to the reservoir by means of an injection well and recovered from the reservoir by means of a production well. Water is continuously provided to the reservoir and continuously withdrawn from the reservoir at two different flow rates, a base rate and a peak rate. Increasing water flow from the base rate to the peak rate is accomplished by rapidly decreasing backpressure at the outlet of the production well in order to meet periodic needs for amounts of thermal energy greater than a baseload amount, such as to generate additional electric power to meet peak demands. The rate of flow of water provided to the hot dry rock reservoir is maintained at a value effective to prevent depletion of the liquid

Delivery of salmon calcitonin using a microneedle patch

PubMed Central

Tas, Cetin; Mansoor, Saffar; Kalluri, Haripriya; Zarnitsyn, Vladimir G.; Choi, Seong-O; Banga, Ajay K.; Prausnitz, Mark R.

2011-01-01

Peptides and polypeptides have important pharmacological properties but only a limited number have been exploited as therapeutics because of problems related to their delivery. Most of these drugs require a parenteral delivery system which introduces the problems of pain, possible infection, and expertise required to carry out an injection. The aim of this study was to develop a transdermal patch containing microneedles (MNs) coated with a peptide drug, salmon calcitonin (sCT), as an alternative to traditional subcutaneous and nasal delivery routes. Quantitative analysis of sCT after coating and drying onto microneedles was performed with a validated HPLC method. In vivo studies were carried out on hairless rats and serum levels of sCT were determined by ELISA. The AUC value of MNs coated with a trehalose-containing formulation (250 ± 83 ng/mL.min) was not significantly different as compared to subcutaneous injections (403 ± 253 ng/mL.min), but approximately 13 times higher than nasal administration (18.4 ± 14.5 ng/mL.min). Tmax (7.5 ± 5 min) values for MN mediated administration were 50% shorter than subcutaneous injections (15 min), possibly due to rapid sCT dissolution and absorption by dermal capillaries. These results suggest that with further optimization of coating formulations, microneedles may enable administration of sCT and other peptides without the need for hypodermic injections. PMID:22172290

Preparation, in vitro release, and pharmacokinetics in rabbits of lyophilized injection of sorafenib solid lipid nanoparticles

PubMed Central

Zhang, Hong; Zhang, Fu-Ming; Yan, Shi-Jun

2012-01-01

Sorafenib solid lipid nanoparticles (S-SLN) were prepared by emulsion evaporation–solidification at low temperature. Morphology was examined by transmission electron microscope. Particle size and zeta potential were determined by laser granularity equipment. Encapsulation efficiency (EE) was detected by Sephadex gel chromatography and high-performance liquid chromatography (HPLC). The in vitro release profile of S-SLN was studied with dialysis technology. The lyophilized injection of S-SLN was prepared by freeze drying and analyzed by differential scanning calorimetry. The plasma concentration of sorafenib in blood was determined by HPLC. The solid lipid nanoparticles assumed a spherical shape with an even distribution of diameter and particle size 108.23 ± 7.01 nm (n = 3). The polydispersity index, zeta potential, and EE were determined to be 0.25 ± 0.02, −16.37 ± 0.65 mV, and 93.49% ± 1.87%, respectively (n = 3). The in vitro release accorded with the Weibull distribution model. An equal volume of 15% (w/v) mannitol performed better as the protective agent for a lyophilized injection of S-SLN with a new material phase formation. The pharmacokinetic processes of sorafenib solution and lyophilized injection of S-SLN in vivo were in accordance with the two-compartment and one-compartment models, respectively. S-SLN nanoparticles are thus considered a promising drug-delivery system. PMID:22787390

Autophagy regulating kinases as potential therapeutic targets for age-related macular degeneration.

PubMed

Kaarniranta, Kai; Kauppinen, Anu; Blasiak, Janusz; Salminen, Antero

2012-11-01

Age-related macular degeneration (AMD) is the leading cause of central vision loss in the elderly in the developed countries. The number of AMD patients will double during the next decades due to increasing number of aged people. Chronic oxidative stress, inflammation and accumulation of protein-rich deposits both in the retinal pigment epithelium lysosomes and under the retinal pigment epithelium herald the onset of AMD. The disease can be divided into dry and wet AMD forms. The dry form of the disease is more prevalent accounting for up to 90% of all cases. Continued intraocular injections are the current treatment strategy to prevent progression of wet AMD. It is a major challenge to develop new drugs that could prevent or at least ease the symptoms of the increasing population of AMD patients. Since AMD pathology is clearly associated with accumulated protein deposits, the autophagy clearance system might represent a potential future therapeutic target for AMD as is thoroughly discussed here.

Hybrid Hydroxyapatite Nanoparticle Colloidal Gels are Injectable Fillers for Bone Tissue Engineering

PubMed Central

Gu, Zhen; Jamal, Syed; Detamore, Michael S.

2013-01-01

Injectable bone fillers have emerged as an alternative to the invasive surgery often required to treat bone defects. Current bone fillers may benefit from improvements in dynamic properties such as shear thinning during injection and recovery of material stiffness after placement. Negatively charged inorganic hydroxyapatite (HAp) nanoparticles (NPs) were assembled with positively charged organic poly(d,l-lactic-co-glycolic acid) (PLGA) NPs to create a cohesive colloidal gel. This material is held together by electrostatic forces that may be disrupted by shear to facilitate extrusion, molding, or injection. Scanning electron micrographs of the dried colloidal gels showed a well-organized, three-dimensional porous structure. Rheology tests revealed that certain colloidal gels could recover after being sheared. Human umbilical cord mesenchymal stem cells were also highly viable when seeded on the colloidal gels. HAp/PLGA NP colloidal gels offer an attractive scheme for injectable filling and regeneration of bone tissue. PMID:23815275

Effects of volcanic eruptions on China's monsoon precipitation over the past 700 years

NASA Astrophysics Data System (ADS)

Zhuo, Z.; Gao, C.

2013-12-01

Tropical volcanic eruptions were found to affect precipitation especially in Asia and Africa monsoon region. However, studies with different types of eruptions suggested different impacts as well as the spatial patterns. In this study, we combined the Monsoon Asia Drought Atlas (MADA, [Cook et al., 2010]) and the Chinese Historical Drought Disaster Index (CHDDI) compiled from the historic meteorological records to study the effect of volcanic eruptions on China's monsoon precipitation over the past 700 years. Histories of past volcanism were compiled from the IVI2[Gao et al., 2008] and Crowley2013[Crowley and Unterman, 2013] reconstructions. Volcanic events were classified into 2×Pinatubo, 1×Pinatubo , ≥5 Tg sulfate aerosols injection in the northern hemisphere (NH) stratosphere for IVI2; and NH sulfate flux more than 20/15/10/5 kg km-2 for Crowley2013. In both cases, average MADA show a drying trend over mainland China from year zero(0) to year three(+3) after the eruption; and the more sulfate aerosol injected into the NH stratosphere or the larger the sulfate flux, the more severe this drying trend seem to reveal. In comparison, a wetting trend was found in the eruption year with Southern Hemisphere (SH) only injections. Superposed epoch analysis with a 10,000 Monte Carlo resampling procedure showed that 97.9% (96.9%) of the observed MADA values are statistically significant at the 95% (99%) confidence level. The drying is probably caused by a reduction of the latent heat flux due to volcanic aerosol' cooling effect, leading to the weakening of south Asian monsoon and decrease of moisture vapor over tropical oceans, which contribute to a reduced moisture flux over china. Spatial distribution of the average MADA show a southward movement of the driest areas in eastern China from year zero to year three after the 1×Pinatubo and 2×Pinatubo eruptions, whereas part of north china experienced unusual wetting condition. This is in good agreement with CHDDI, which illustrates the effectiveness of MADA in reflecting China's hydrological condition during the summer monsoon season. On the other hand, with only SH injection, north and east china turn to wet in the eruption year and show a southward movement of the wettest areas, when compared to NH injection more than 2×Pinatubo. This spatial difference may shed some light on the possible effects stratospheric geoengineering may have on China's precipitation. References: Cook, E. R., et al. (2010), Asian Monsoon Failure and Megadrought During the Last Millennium, Science, 328(5977), 486-489. Crowley, T. J., and M. B. Unterman (2013), Technical details concerning development of a 1200 yr proxy index for global volcanism, Earth System Science Data, 5(1), 187-197. Gao, C. C., et al. (2008), Volcanic forcing of climate over the past 1500 years: An improved ice core-based index for climate models, J Geophys Res, 113(D23111D23).

Solid lipid nanoparticles as insulin inhalation carriers for enhanced pulmonary delivery.

PubMed

Bi, Ru; Shao, Wei; Wang, Qun; Zhang, Na

2009-02-01

Growing attentions have been paid to the pulmonary route for systemic delivery of peptide and protein drugs, such as insulin. Advantages of this non-injective route include rapid drug deposition in the target organ, fewer systemic side effects and avoiding first pass metabolism. However, sustained release formulations for pulmonary delivery have not been fully exploited till now. In our study, a novel dry powder inhalation (DPI) system of insulin loaded solid lipid nanoparticles (Ins-SLNs) was investigated for prolonged drug release, improved stability and effective inhalation. Firstly, the drug was incorporated into the lipid carriers for a maximum entrapment efficiency as high as 69.47 +/- 3.27% (n = 3). Secondly, DPI formulation was prepared by spray freeze drying of Ins-SLNs suspension, with optimized lyoprotectant and technique parameters in this procedure. The properties of DPI particles were characterized for their pulmonary delivery potency. Thirdly, the in vivo study of intratracheal instillation of Ins-SLNs to diabetic rats showed prolonged hypoglycemic effect and a relative pharmacological bioavailability of 44.40% could be achieved in the group of 8 IU/kg dosage. These results indicated that SLNs have shown increasing potential as an efficient and non-toxic lipophilic colloidal drug carrier for enhanced pulmonary delivery of insulin.

Probable Mechanisms of Needling Therapies for Myofascial Pain Control

PubMed Central

Chou, Li-Wei; Kao, Mu-Jung; Lin, Jaung-Geng

2012-01-01

Myofascial pain syndrome (MPS) has been defined as a regional pain syndrome characterized by muscle pain caused by myofascial trigger points (MTrPs) clinically. MTrP is defined as the hyperirritable spot in a palpable taut band of skeletal muscle fibers. Appropriate treatment to MTrPs can effectively relieve the clinical pain of MPS. Needling therapies, such as MTrP injection, dry needling, or acupuncture (AcP) can effectively eliminate pain immediately. AcP is probably the first reported technique in treating MPS patients with dry needling based on the Traditional Chinese Medicine (TCM) theory. The possible mechanism of AcP analgesia were studied and published in recent decades. The analgesic effect of AcP is hypothesized to be related to immune, hormonal, and nervous systems. Compared to slow-acting hormonal system, nervous system acts in a faster manner. Given these complexities, AcP analgesia cannot be explained by any single mechanism. There are several principles for selection of acupoints based on the TCM principles: “Ah-Shi” point, proximal or remote acupoints on the meridian, and extra-meridian acupoints. Correlations between acupoints and MTrPs are discussed. Some clinical and animal studies of remote AcP for MTrPs and the possible mechanisms of remote effectiveness are reviewed and discussed. PMID:23346211

Azadirachtin: an effective systemic insecticide for control of Agrilus planipennis (Coleoptera: Buprestidae).

PubMed

McKenzie, Nicole; Helson, Blair; Thompson, Dean; Otis, Gard; McFarlane, John; Buscarini, Teresa; Meating, Joe

2010-06-01

The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive pest discovered in North America in 2002, is now well established and threatens ash (Fraxinus spp.) trees throughout the continent. Experiments were conducted to 1) examine the efficacy of an alternative natural pesticide, azadirachtin, to control emerald ash borer, and 2) determine foliar uptake and dissipation patterns after systemic injections of azadirachtin into trunks of small (2.2 cm diameter at breast height [dbh]), uninfested green ash trees. We found no evidence of mortality of adult beetles. In contrast, fewer larvae completed their development at dose levels > or = 1.7 mg (AI)/cm dbh and development ceased beyond the second instar at dose levels > or = 13.6 mg (AI)/cm dbh. Substantial concentrations (11.2 microg/g dry mass [SD = 7.55]) of azadirachtin were present in leaves within 7 d of treatment. After rapid initial uptake, concentrations in leaves declined logarithmically during the 55 d after injection. A similar pattern was observed in a separate experiment that examined the uptake and translocation of azadirachtin in larger green ash trees (22 cm dbh) treated with 250 mg (AI) /cm dbh with the EcoJect injection system. In another experiment, recently infested plantation green ash trees treated with doses > or = 40 mg (AI)/cm dbh had significant reductions in adult emergence approximately 1 yr postinjection. Given the inhibition of larval development, reduction of adult emergence, and the occurrence of foliar residues at biologically active concentrations, we conclude that azadirachtin is effective in protecting ash trees from emerald ash borer.

Is Limited Prehospital Resuscitation with Plasma More Beneficial than Using a Synthetic Colloid? An Experimental Study in Rabbits with Parenchymal Bleeding

DTIC Science & Technology

2015-04-01

pediatric TBI patients.36,37 The albumin solution used in this rabbit study was a 5% human albumin (Albminar) in an isotonic solution ( normal saline ... reconstituted easily at the point of injury and injected . Hemostatic properties and bene- fits of FDP or sprayed-dried plasma have been demonstrated...rabbit albumin solution appro- priate for intravenous injection is not commercially available. Rabbit albumin is sold by chemical companies as

Experimental analysis for fabrication of high-aspect-ratio piezoelectric ceramic structure by micro-powder injection molding process

NASA Astrophysics Data System (ADS)

Han, Jun Sae; Gal, Chang Woo; Park, Jae Man; Kim, Jong Hyun; Park, Seong Jin

2018-04-01

Aspect ratio effects in the micro-powder injection molding process were experimentally analyzed for fabrication of high-aspect-ratio piezoelectric ceramic structure. The mechanisms of critical defects have been studied according to individual manufacturing steps. In the molding process, incomplete filling phenomenon determines the critical aspect ratios of a micro pattern. According to mold temperature, an incomplete filling phenomenon has been analyzed with respect to different pattern sizes and aspect ratio. In demolding and drying process, the capillary behavior of sacrificial polymeric mold insert determines the critical aspect ratio of a micro pattern. With respect to pattern dimensions, slumping behavior has been analyzed. Based on our current systems, micro PZT feature has stability when it has lower aspect ratio than 5. Under optimized processing conditions, 20 μm and 40 μm ceramic rod array feature which has 5 of aspect ratio were successfully fabricated by the developed process. Further modification points to fabricate the smaller and higher feature were specifically addressed.

Sustainable in-well vapor stripping: A design, analytical model, and pilot study for groundwater remediation

NASA Astrophysics Data System (ADS)

Sutton, Patrick T.; Ginn, Timothy R.

2014-12-01

A sustainable in-well vapor stripping system is designed as a cost-effective alternative for remediation of shallow chlorinated solvent groundwater plumes. A solar-powered air compressor is used to inject air bubbles into a monitoring well to strip volatile organic compounds from a liquid to vapor phase while simultaneously inducing groundwater circulation around the well screen. An analytical model of the remediation process is developed to estimate contaminant mass flow and removal rates. The model was calibrated based on a one-day pilot study conducted in an existing monitoring well at a former dry cleaning site. According to the model, induced groundwater circulation at the study site increased the contaminant mass flow rate into the well by approximately two orders of magnitude relative to ambient conditions. Modeled estimates for 5 h of pulsed air injection per day at the pilot study site indicated that the average effluent concentrations of dissolved tetrachloroethylene and trichloroethylene can be reduced by over 90% relative to the ambient concentrations. The results indicate that the system could be used cost-effectively as either a single- or multi-well point technology to substantially reduce the mass of dissolved chlorinated solvents in groundwater.

Investigation of Fragment Antibody Stability and Its Release Mechanism from Poly(Lactide-co-Glycolide)-Triacetin Depots for Sustained-Release Applications.

PubMed

Chang, Debby P; Garripelli, Vivek Kumar; Rea, Jennifer; Kelley, Robert; Rajagopal, Karthikan

2015-10-01

Achieving long-term drug release from polymer-based delivery systems continues to be a challenge particularly for the delivery of large hydrophilic molecules such as therapeutic antibodies and proteins. Here, we report on the utility of an in situ-forming and injectable polymer-solvent system for the long-term release of a model antibody fragment (Fab1). The delivery system was prepared by dispersing a spray-dried powder of Fab1 within poly(lactide-co-glycolide) (PLGA)-triacetin solution. The formulation viscosity was within the range 1.0 ± 0.3 Pa s but it was injectable through a 27G needle. The release profile of Fab1, measured in phosphate-buffered saline (PBS), showed a lag phase followed by sustained-release phase for close to 80 days. Antibody degradation during its residence within the depot was comparable to its degradation upon long-term incubation in PBS. On the basis of temporal changes in surface morphology, stiffness, and depot mass, a mechanism to account for the drug release profile has been proposed. The unprecedented release profile and retention of greater than 80% of antigen-binding capacity even after several weeks demonstrates that PLGA-triacetin solution could be a promising system for the long-term delivery of biologics. © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association.

Measles vaccination using a microneedle patch☆

PubMed Central

Edens, Chris; Collins, Marcus L.; Ayers, Jessica; Rota, Paul A.; Prausnitz, Mark R.

2013-01-01

Measles vaccination programs would benefit from delivery methods that decrease cost, simplify logistics, and increase safety. Conventional subcutaneous injection is limited by the need for skilled healthcare professionals to reconstitute and administer injections, and by the need for safe needle handling and disposal to reduce the risk of disease transmission through needle re-use and needlestick injury. Microneedles are micron-scale, solid needles coated with a dry formulation of vaccine that dissolves in the skin within minutes after patch application. By avoiding the use of hypodermic needles, vaccination using a microneedle patch could be carried out by minimally trained personnel with reduced risk of blood-borne disease transmission. The goal of this study was to evaluate measles vaccination using a microneedle patch to address some of the limitations of subcutaneous injection. Viability of vaccine virus dried onto a microneedle patch was stabilized by incorporation of the sugar, trehalose, and loss of viral titer was less than 1 log10(TCID50) after storage for at least 30 days at room temperature. Microneedle patches were then used to immunize cotton rats with the Edmonston-Zagreb measles vaccine strain. Vaccination using microneedles at doses equaling the standard human dose or one-fifth the human dose generated neutralizing antibody levels equivalent to those of a subcutaneous immunization at the same dose. These results show that measles vaccine can be stabilized on microneedles and that vaccine efficiently reconstitutes in vivo to generate a neutralizing antibody response equivalent to that generated by subcutaneous injection. PMID:23044406

Determination of paraben preservatives in seafood using matrix solid-phase dispersion and on-line acetylation gas chromatography-mass spectrometry.

PubMed

Djatmika, Rosalina; Hsieh, Chih-Chung; Chen, Jhih-Ming; Ding, Wang-Hsien

2016-11-15

An effective method for determining four commonly detected paraben preservatives (methyl, ethyl, propyl and butyl paraben) in marketed seafood is presented. This method employs matrix solid-phase dispersion (MSPD) before identification and quantification of the paraben preservatives via on-line acetylation gas chromatography-mass spectrometry (GC-MS). Parameters affecting the extraction efficiency of MSPD were optimized through a Box-Behnken design method. Under optimal condition, 0.5-g of freeze-dried seafood was mixed with 0.5-g of anhydrous sodium sulfate, and dispersed with 1.0-g of Florisil using vortex. After that, the blend was transferred to a glass column containing 1.5-g of silica gel+C18 (w/w, 9:1), which acted as clean-up co-sorbents. Then, target analytes were eluted with 12mL of acetonitrile. The extract was then derivatized on-line in the GC injection-port through reaction with acetic anhydride, and the identity and quantity of the target analytes were determined by the GC-MS system. The limits of quantitation (LOQs) were 0.2 to 1.0ng/g (dry weight). Preliminary results showed that the total concentrations of four selected parabens ranged from 16.7 to 44.7ng/g (dry weight). Copyright © 2016 Elsevier B.V. All rights reserved.

Newberry Well 55-29 Stimulation Data 2014

DOE Data Explorer

Trenton T. Cladouhos

2015-09-03

The Newberry Volcano EGS Demonstration in central Oregon, a 5 year project begun in 2010, tests recent technological advances designed to reduce the cost of power generated by EGS in a hot, dry well (NWG 55-29) drilled in 2008. First, the stimulation pumps used were designed to run for weeks and deliver large volumes of water at moderate well-head pressure. Second, to stimulate multiple zones, AltaRock developed thermo-degradable zonal isolation materials (TZIMs) to seal off fractures in a geothermal well to stimulate secondary and tertiary fracture zones. The TZIMs degrade within weeks, resulting in an optimized injection/ production profile of the entire well. Third, the project followed a project-specific Induced Seismicity Mitigation Plan (ISMP) to evaluate, monitor for, and mitigate felt induced seismicity. An initial stimulation was conducted in 2012 and continued for 7 weeks, with over 41,000 m3 of water injected. Further analysis indicated a shallow casing leak and an unstable formation in the open hole. The well was repaired with a shallow casing tieback and perforated liner in the open hole and re-stimulated in 2014. The second stimulation started September 23rd, 2014 and continued for 3 weeks with over 9,500 m3 of water injected. The well was treated with several batches of newly tested TZIM diverter materials and a newly designed Diverter Injection Vessel Assembly (DIVA), which was the main modification to the original injection system design used in 2012. A second round of stimulation that included two perforation shots and additional batches of TZIM was conducted on November 11th, 2014 for 9 days with an additional 4,000 m3 of water injected. The stimulations resulted in a 3-4 fold increase in injectivity, and PTS data indicates partial blocking and creation of flow zones near the bottom of the well.

Mass Balance Analysis of Contaminated Heparin Product

PubMed Central

Liu, Zhenling; Xiao, Zhongping; Masuko, Sayaka; Zhao, Wenjing; Sterner, Eric; Bansal, Vinod; Fareed, Jawed; Dordick, Jonathan; Zhang, Fuming; Linhardt, Robert J.

2011-01-01

A quantitative analysis of a recalled contaminated lot of heparin (HP) sodium injection United States Pharmacopeial (USP) was undertaken in response to the controversy regarding the exact nature of the contaminant involved in the HP crisis. A mass balance analysis of the formulated drug product was performed. After freeze-drying, a 1 ml vial for injection afforded 54.8 ± 0.3 mg of dry solids. The excipients, sodium chloride and residual benzyl alcohol, accounted for 11.4 ± 0.5 mg and 0.9 ± 0.5 mg, respectively. Active pharmaceutical ingredient (API) represented 41.5 ± 1.0 mg, corresponding to 75.7 wt% of dry mass. Exhaustive treatment of API with specific enzymes, heparin lyases and/or chondroitin lyases was used to close mass balance. HP represented 30.5 ± 0.5 mg, corresponding to 73.5 wt% of the API. Dermatan sulfate (DS) impurity represented 1.7 ± 0.3 mg, corresponding to 4.1 wt% of the API. Contaminant, 9.3 ± 0.1 mg corresponding to 22.4 wt% of API, was found in the contaminated formulated drug product. The recovery of contaminant was close to quantitative (95.6-100 wt%). A single contaminant was unambiguously identified as oversulfated chondroitin sulfate (OSCS). PMID:20850409

Nanoscale surface characterization and miscibility study of a spray-dried injectable polymeric matrix consisting of poly(lactic-co-glycolic acid) and polyvinylpyrrolidone.

PubMed

Meeus, Joke; Chen, Xinyong; Scurr, David J; Ciarnelli, Valeria; Amssoms, Katie; Roberts, Clive J; Davies, Martyn C; van Den Mooter, Guy

2012-09-01

Injectable controlled-release formulations are of increasing interest for the treatment of chronic diseases. This study aims to develop and characterize a polymeric matrix for intramuscular or subcutaneous injection, consisting of two biocompatible polymers, particularly suitable for formulating poorly soluble drugs. For this matrix, the water-insoluble polymer poly(lactic-co-glycolic acid) (PLGA) is combined with the water-soluble polymer polyvinylpyrrolidone (PVP). Microparticles of these two polymers were prepared by spray drying. The phase behavior of the samples was studied by means of modulated differential scanning calorimetry and the results showed that phase separation occurred in the bulk sample through evidence of two mixed amorphous phases, namely, a PLGA-rich phase and a PVP-rich phase. Characterization of the samples by scanning electron microscopy demonstrated that the spray-dried particles were hollow with a thin shell. Because of the importance in relation to stability and drug release, information about the surface of the microparticles was collected by different complementary surface analysis techniques. Atomic force microscopy gathered information about the morphology and phase behavior of the microparticle surface. Time-of-flight secondary ion mass spectrometry analysis of the particles revealed that the surface consisted mainly of the PLGA-rich phase. This was confirmed by X-ray photoelectron spectroscopy at an increased sampling depth (≈ 10 nm). Nanothermal analysis proved to be an innovative way to thermally detect the presence of the PLGA-dominated surface layer and the underlying PVP phase. Taken together, this information provides a rational basis for predicting the likely drug release behavior this formulation will display. Copyright © 2012 Wiley Periodicals, Inc.

Effects of Multiple Routes of Cadmium Exposure on the Hibernation Success of the American Toad (Bufo americanus)

USGS Publications Warehouse

James, S.M.; Little, E.E.; Semlitsch, R.D.

2004-01-01

The effects of multiple routes of cadmium exposure on juvenile American toads (Bufo americanus) were evaluated using environmentally relevant concentrations. During or after exposure, toads were individually hibernated for 172 days at approximately 4??C. The following experiments were conducted: (1) dermal exposure (hibernation in soil contaminated with up to 120 ??g Cd/ g (dry weight)); (2) injection exposure (single injection with cadmium to achieve a maximum whole-body nominal concentration of 3 ??g Cd/g (wet weight) 12 days before hibernation in uncontaminated soil); and, (3) oral exposure (feeding with mealworms containing ???16 ??g Cd/g (dry weight) for 50 days before hibernation in uncontaminated soil)., We hypothesized that sublethal levels of cadmium would become lethal during hibernation because of combined chemical and cold stress. No prehibernation mortality occurred in the injection and oral exposure studies. There was a significant treatment effect on whole-body cadmium concentration in toads orally or dermally exposed and on percent of cadmium retention in toads orally exposed. There was also a trend of increased time-to-burrowing and more toads partially buried with greater cadmium concentration in the dermal study, which indicated avoidance. In all 3 experiments, no significant differences were found among cadmium treatments in hibernation survival, percent of mass loss, or locomotor performance. However, toads fed mealworms averaging 4.7 ??g Cd/g (dry weight) had only 56% survival compared with 100% survival for controls. Although our results suggest that environmentally relevant levels of cadmium do not pose a great risk to American toads, factors such as soil type or prey species may increase cadmium bioavailability, and other amphibian species may be more sensitive to cadmium than B. americanus.

Systemic Delivery of Atropine Sulfate by the MicroDose Dry-Powder Inhaler

PubMed Central

Venkataramanan, R.; Hoffman, R.M.; George, M.P.; Petrov, A.; Richards, T.; Zhang, S.; Choi, J.; Gao, Y.Y.; Oakum, C.D.; Cook, R.O.; Donahoe, M.

2013-01-01

Abstract Background Inhaled atropine is being developed as a systemic and pulmonary treatment for the extended recovery period after chemical weapons exposure. We performed a pharmacokinetics study comparing inhaled atropine delivery using the MicroDose Therapeutx Dry Powder Inhaler (DPIA) with intramuscular (IM) atropine delivery via auto-injector (AUTO). Methods The MicroDose DPIA utilizes a novel piezoelectric system to aerosolize drug and excipient from a foil dosing blister. Subjects inhaled a 1.95-mg atropine sulfate dose from the dry powder inhaler on one study day [5 doses×0.4 mg per dose (nominal) delivered over 12 min] and received a 2-mg IM injection via the AtroPen® auto-injector on another. Pharmacokinetics, pharmacodynamic response, and safety were studied for 12 hr. Results A total of 17 subjects were enrolled. All subjects completed IM dosing. One subject did not perform inhaled delivery due to a skin reaction from the IM dose. Pharmacokinetic results were as follows: area under the curve concentration, DPIA=20.1±5.8, AUTO=23.7±4.9 ng hr/mL (means±SD); maximum concentration reached, DPIA=7.7±3.5, AUTO=11.0±3.8 ng/mL; time to reach maximum concentration, DPIA=0.25±0.47, AUTO=0.19±0.23 hr. Pharmacodynamic results were as follows: maximum increase in heart rate, DPIA=18±12, AUTO=23±13 beats/min; average change in 1-sec forced expiratory volume at 30 min, DPIA=0.16±0.22 L, AUTO=0.11±0.29 L. The relative bioavailability for DPIA was 87% (based on output dose). Two subjects demonstrated allergic responses: one to the first dose (AUTO), which was mild and transient, and one to the second dose (DPIA), which was moderate in severity, required treatment with oral and intravenous (IV) diphenhydramine and IV steroids, and lasted more than 7 days. Conclusions Dry powder inhalation is a highly bioavailable route for attaining rapid and consistent systemic concentrations of atropine. PMID:22691110

Systemic delivery of atropine sulfate by the MicroDose Dry-Powder Inhaler.

PubMed

Corcoran, T E; Venkataramanan, R; Hoffman, R M; George, M P; Petrov, A; Richards, T; Zhang, S; Choi, J; Gao, Y Y; Oakum, C D; Cook, R O; Donahoe, M

2013-02-01

Inhaled atropine is being developed as a systemic and pulmonary treatment for the extended recovery period after chemical weapons exposure. We performed a pharmacokinetics study comparing inhaled atropine delivery using the MicroDose Therapeutx Dry Powder Inhaler (DPIA) with intramuscular (IM) atropine delivery via auto-injector (AUTO). The MicroDose DPIA utilizes a novel piezoelectric system to aerosolize drug and excipient from a foil dosing blister. Subjects inhaled a 1.95-mg atropine sulfate dose from the dry powder inhaler on one study day [5 doses × 0.4 mg per dose (nominal) delivered over 12 min] and received a 2-mg IM injection via the AtroPen® auto-injector on another. Pharmacokinetics, pharmacodynamic response, and safety were studied for 12 hr. A total of 17 subjects were enrolled. All subjects completed IM dosing. One subject did not perform inhaled delivery due to a skin reaction from the IM dose. Pharmacokinetic results were as follows: area under the curve concentration, DPIA=20.1±5.8, AUTO=23.7±4.9 ng hr/mL (means±SD); maximum concentration reached, DPIA=7.7±3.5, AUTO=11.0±3.8 ng/mL; time to reach maximum concentration, DPIA=0.25±0.47, AUTO=0.19±0.23 hr. Pharmacodynamic results were as follows: maximum increase in heart rate, DPIA=18±12, AUTO=23±13 beats/min; average change in 1-sec forced expiratory volume at 30 min, DPIA=0.16±0.22 L, AUTO=0.11±0.29 L. The relative bioavailability for DPIA was 87% (based on output dose). Two subjects demonstrated allergic responses: one to the first dose (AUTO), which was mild and transient, and one to the second dose (DPIA), which was moderate in severity, required treatment with oral and intravenous (IV) diphenhydramine and IV steroids, and lasted more than 7 days. Dry powder inhalation is a highly bioavailable route for attaining rapid and consistent systemic concentrations of atropine.

Deposition and fine particle production during dynamic flow in a dry powder inhaler: a CFD approach.

PubMed

Milenkovic, J; Alexopoulos, A H; Kiparissides, C

2014-01-30

In this work the dynamic flow as well as the particle motion and deposition in a commercial dry powder inhaler, DPI (i.e., Turbuhaler) is described using computational fluid dynamics, CFD. The dynamic flow model presented here is an extension of a steady flow model previously described in Milenkovic et al. (2013). The model integrates CFD simulations for dynamic flow, an Eulerian-fluid/Lagrangian-particle description of particle motion as well as a particle/wall interaction model providing the sticking efficiency of particles colliding with the DPI walls. The dynamic flow is imposed by a time varying outlet pressure and the particle injections into the DPI are assumed to occur instantaneously and follow a prescribed particle size distribution, PSD. The total particle deposition and the production of fine particles in the DPI are determined for different peak inspiratory flow rates, PIFR, flow increase rates, FIR, and particle injection times. The simulation results for particle deposition are found to agree well with available experimental data for different values of PIFR and FIR. The predicted values of fine particle fraction are in agreement with available experimental results when the mean size of the injected PSD is taken to depend on the PIFR. Copyright © 2013 Elsevier B.V. All rights reserved.

Pentostatin Injection

MedlinePlus

... pain constipation sores in the mouth and throat flatulence or large amounts of gas in the intestines or bowels hair loss muscle, back, or joint pain headache sweating difficulty falling asleep or staying asleep dry skin itching loss of strength or energy Some side effects can be serious. If you ...

Processing of sintered alpha SiC

NASA Technical Reports Server (NTRS)

Storm, R. S.

1984-01-01

Processing methods of sintered alpha SiC for engine applications are developed in a cost effective manner, using a submicron sized powder blended with sintering aids (boron and carbon). The processes for forming a green powder compact, such as dry pressing, cold isostatic pressing and green machining, slip casting, aqueous extrusion, plastic extrusion, and injection molding, are described. Dry pressing is the simplest route to component fabrication, and is carried out at approximately 10,000 psi pressure, while in the cold isostatic method the pressure could go as high as 20,000 psi. Surfactants are added to control settling rates and casting characteristics in the slip casting. The aqueous extrusion process is accomplished by a hydraulic ram forcing the aqueous mixture through a die. The plastic forming processes of extrusion and injection molding offer the potential of greater diversity in shape capacity. The physical properties of sintered alpha SiC (hardness, Young's modulus, shear modulus, and thermal diffusivity) are extensively tested. Corrosion resistance test results of silicon carbide are included.

Efficient Permeability Measurement and Numerical Simulation of the Resin Flow in Low Permeability Preform Fabricated by Automated Dry Fiber Placement

NASA Astrophysics Data System (ADS)

Agogue, Romain; Chebil, Naziha; Deleglise-Lagardere, Mylène; Beauchene, Pierre; Park, Chung Hae

2017-10-01

We propose a new experimental method using a Hassler cell and air injection to measure the permeability of fiber preform while avoiding a race tracking effect. This method was proven to be particularly efficient to measure very low through-thickness permeability of preform fabricated by automated dry fiber placement. To validate the reliability of the permeability measurement, the experiments of viscous liquid infusion into the preform with or without a distribution medium were performed. The experimental data of flow front advancement was compared with the numerical simulation result using the permeability values obtained by the Hassler cell permeability measurement set-up as well as by the liquid infusion experiments. To address the computational cost issue, the model for the equivalent permeability of distribution medium was employed in the numerical simulation of liquid flow. The new concept using air injection and Hassler cell for the fiber preform permeability measurement was shown to be reliable and efficient.

Comparison of postoperative corneal changes between dry eye and non-dry eye in a murine cataract surgery model.

PubMed

Kwon, Jin Woo; Chung, Yeon Woong; Choi, Jin A; La, Tae Yoon; Jee, Dong Hyun; Cho, Yang Kyung

2016-01-01

To compare the effects of the surgical insult of cataract surgery on corneal inflammatory infiltration, neovascularization (NV) and lymphangiogenesis (LY) between the dry eye and non-dry eye in murine cataract surgery models. We established two groups of animals, one with normal eyes (non-dry eye) and the second with induced dry eyes. In both groups, we used surgical insults to mimic human cataract surgery, which consisted of lens extraction, corneal incision and suture. After harvesting of corneas on the 9(th) postoperative day and immunohistochemical staining, we compared NV, LY and CD11b+ cell infiltration in the corneas. Dry eye group had significantly more inflammatory infiltration (21.75%±7.17% vs 3.65%±1.49%; P=0.049). The dry eye group showed significantly more NV (48.21%±4.02% vs 26.24%±6.01%; P=0.016) and greater levels of LY (9.27%±0.48% vs 4.84%±1.15%; P=0.007). In corneas on which no surgery was performed, there was no induction of NV in both the dry and non-dry group, but dry eye group demonstrated more CD11b+ cells infiltration than the non-dry eye group (0.360%±0.160% vs 0.023%±0.006%; P=0.068). Dry eye group showed more NV than non-dry eye group in both topical PBS application and subconjunctival PBS injection (P=0.020 and 0.000, respectively). In a murine cataract surgery model, preexisting dry eye can induce more postoperative NV, LY, and inflammation in corneal tissue.

Comparison of postoperative corneal changes between dry eye and non-dry eye in a murine cataract surgery model

PubMed Central

Kwon, Jin Woo; Chung, Yeon Woong; Choi, Jin A; La, Tae Yoon; Jee, Dong Hyun; Cho, Yang Kyung

2016-01-01

AIM To compare the effects of the surgical insult of cataract surgery on corneal inflammatory infiltration, neovascularization (NV) and lymphangiogenesis (LY) between the dry eye and non-dry eye in murine cataract surgery models. METHODS We established two groups of animals, one with normal eyes (non-dry eye) and the second with induced dry eyes. In both groups, we used surgical insults to mimic human cataract surgery, which consisted of lens extraction, corneal incision and suture. After harvesting of corneas on the 9th postoperative day and immunohistochemical staining, we compared NV, LY and CD11b+ cell infiltration in the corneas. RESULTS Dry eye group had significantly more inflammatory infiltration (21.75%±7.17% vs 3.65%±1.49%; P=0.049). The dry eye group showed significantly more NV (48.21%±4.02% vs 26.24%±6.01%; P=0.016) and greater levels of LY (9.27%±0.48% vs 4.84%±1.15%; P=0.007). In corneas on which no surgery was performed, there was no induction of NV in both the dry and non-dry group, but dry eye group demonstrated more CD11b+ cells infiltration than the non-dry eye group (0.360%±0.160% vs 0.023%±0.006%; P=0.068). Dry eye group showed more NV than non-dry eye group in both topical PBS application and subconjunctival PBS injection (P=0.020 and 0.000, respectively). CONCLUSION In a murine cataract surgery model, preexisting dry eye can induce more postoperative NV, LY, and inflammation in corneal tissue. PMID:26949638

Injection-salting of pre rigor fillets of Atlantic salmon (Salmo salar).

PubMed

Birkeland, Sveinung; Akse, Leif; Joensen, Sjurdur; Tobiassen, Torbjørn; Skåra, Torstein

2007-01-01

The effects of temperature (-1, 4, and 10 degrees C), brine concentration (12% and 25% NaCl), injection volumes, and needle densities were investigated on fillet weight gain (%), salt content (%), fillet contraction (%), and muscle gaping in pre rigor brine-injected fillets of Atlantic salmon (Salmo salar). Increased brine concentration (12% to 25%) significantly increased the initial (< 5 min after injection) and final contraction (24 h after injection) of pre rigor fillets. Increased brine concentration significantly reduced weight gain and increased salt content but had no significant effect on muscle gaping. The temperatures tested did not significantly affect weight gain, fillet contraction, or gaping score. Significant regressions (P < 0.01) between the injection volume and weight gain (range: 2.5% to 15.5%) and salt content (range: 1.7% to 6.5%) were observed for injections of pre rigor fillets. Double injections significantly increased the weight gain and salt content compared to single injections. Initial fillet contraction measured 30 min after brine injection increased significantly (P < 0.01) with increasing brine injection volume but no significant difference in the fillet contraction was observed 12 h after brine injection (range: 7.9% to 8.9%). Brine-injected post rigor control fillets obtained higher weight gain, higher salt content, more muscle gaping, and significantly lower fillet contraction compared to the pre rigor injected fillets. Injection-salting is an applicable technology as a means to obtain satisfactory salt contents and homogenously distribute the salt into the muscle of pre rigor fillets of Atlantic salmon before further processing steps such as drying and smoking.

HIGH-DOSE HIGH-FREQUENCY AFLIBERCEPT FOR RECALCITRANT NEOVASCULAR AGE-RELATED MACULAR DEGENERATION.

PubMed

You, Qi Sheng; Gaber, Raouf; Meshi, Amit; Ramkumar, Hema L; Alam, Mostafa; Muftuoglu, Ilkay Kilic; Freeman, William R

2018-06-01

To determine the efficacy of monthly (0.1 mL/4 mg) aflibercept for refractory neovascular age-related macular degeneration (wet age-related macular degeneration). This was a retrospective interventional case series in which patients with wet age-related macular degeneration were treated with stepwise dose escalation. Nonvitrectomized patients resistant to monthly (Q4W) ranibizumab/bevacizumab were switched to 2 mg aflibercept every 8 weeks. With resistance, they were escalated to Q4W 2 mg aflibercept, then Q4W 4 mg (high dose high frequency, 4Q4W) aflibercept. Resistance was defined as ≥2 recurrences after being dry following ≥3 injections or persistent exudation on treatment of ≥5 injections. Thirty-three eyes of 28 patients were treated with 4Q4W aflibercept and followed for a mean of 16 months. A dry retina (no intraretinal or subretinal fluid) was achieved after initiating 4Q4W aflibercept treatment at a mean of 3.8 months. Central foveal thickness, maximum foveal thickness, intraretinal fluid, subretinal fluid, and retinal pigment detachment height decreased significantly at 1 month after initiating the 4Q4W aflibercept, and the morphologic therapeutic effect was sustained until the last visit. Forty-five percent of eyes had one or more lines of vision improvement. New geographic atrophy developed in 9% of eyes during follow-up. No ocular or systemic adverse events occurred after initiating 4Q4W aflibercept. Intravitreal high-dose high-frequency aflibercept is an effective treatment for patients with refractory wet age-related macular degeneration.

The Northwest Geysers EGS Demonstration Project, California

DOE PAGES

Garcia, Julio; Hartline, Craig; Walters, Mark; ...

2015-09-04

Our project goal is to demonstrate the feasibility of stimulating a deep high-temperature reservoir (HTR) (up to 400 °C, 750 °F). There were two previously abandoned wells, Prati State 31 (PS-31) and Prati 32 (P-32), reopened and deepened to be used as an injection and production doublet to stimulate the HTR. The deepened portions of both wells have conductive temperature gradients of 10 °F/100 ft (182 °C/km), produce connate native fluids and magmatic gas, and the rocks were isotopically unexchanged by meteoric water. The ambient temperature meteoric water injected into these hot dry rocks has evidently created a permeability volume of several cubic kilometers asmore » determined by seismic monitoring. Preliminary isotopic analyses of the injected and produced water indicate that 50–75% of the steam from the created EGS reservoir is injection-derived.« less

Management of lower urinary tract dysfunction: a stepwise approach.

PubMed

Thom, Matthew; Campigotto, Mary; Vemulakonda, Vijaya; Coplen, Douglas; Austin, Paul F

2012-02-01

To evaluate management patterns of lower urinary tract (LUT) dysfunction and establish a treatment algorithm to guide pediatric healthcare providers. 390 children with non-neurogenic LUT dysfunction were followed over 7 months; 115 patients were excluded due to incomplete data. Children were categorized based on presenting complaints and pelvic ultrasound into three groups: daytime urinary incontinence (UI) with complete emptying (CE), UI with incomplete emptying (IE), or IE without UI. Every child underwent behavioral modification (BM) including timed voiding, double voiding, deep breathing, and treatment of constipation if present. BM failures received secondary treatment including medications (alpha blockers, anticholinergics), physical therapy, and/or botulinum toxin type A injection of the external sphincter at a dose of 100 units. BM improved symptoms in 152 (55%): 68% (46% dry), 49% (27% dry), and 59% (29% dry) from the three groups, respectively. Of the 45% who showed no change in symptoms, 98 (80%) improved with addition of medication, the majority (89) after starting alpha blocker therapy. Children with IE responded better to alpha blockers, 83 (77%) compared to 38% with CE, whereas those with CE demonstrated more symptom resolution with anticholinergics, 6 (38%) compared to 13% of those with IE. Only 6 (2%) patients were refractory to non-operative treatment with all showing improvement after injection of botulinum toxin type A, 4 (67%) of whom became completely dry. Diagnosis of UI and/or IE with stratification of children into particular symptom groups appears beneficial in determining the appropriate therapy for children with LUT dysfunction. Copyright © 2011 Journal of Pediatric Urology Company. Published by Elsevier Ltd. All rights reserved.

Increased negatively of interstitial fluid pressure in rat skin contributes to the edema formation induced by Zymosan.

PubMed

Ostgaard, G; Reed, R K

1993-11-01

Increased negatively of interstitial fluid pressure (Pif) contributes to rapid edema formation in several acute inflammatory reactions attesting to an "active" role for the loose connective tissues in the transcapillary fluid exchange and edema formation under these circumstances. The present study reports the effect of the complement activator Zymosan on Pif, transcapillary fluid, and albumin flux. Micropipettes (tip diameter 5 to 7 microns) connected to a servo-controlled counterpressure system were used to measure Pif in rat dermis. When compared to saline injection, subdermal injection of 1 mg Zymosan in 10 microliters 0.15 M NaCl increased total tissue water by 1.6 ml/g dry weight in 5 min, corresponding to about 150% increase in interstitial fluid volume. Pif increased from +0.4 to +3.7 mm Hg. Increased negativity of Pif can be masked by the edema formation which will increase Pif. Measurements were therefore also performed after circulatory arrest, when transcapillary fluid flux and edema formation are abolished. Using this experimental protocol Pif fell from +0.3 mm Hg to -2.5 mm Hg 5 min after subdermal injection of Zymosan and remained at this level throughout the observation period of 90 min. Injection of saline alone after circulatory arrest increased Pif transiently by about 1 mm Hg. Thus, subdermal injection of Zymosan causes increased negativity of Pif by about 4 mm Hg. Although the lowering of Pif itself will explain a minor part of the increased fluid filtration, the results attest to the role of loose connective tissues being active in the edema-generating process also in the inflammatory reaction induced by Zymosan.

Giving an insulin injection

MedlinePlus

... of Insulin To fill a syringe with one type of insulin: Wash your hands with soap and water. Dry them well. Check ... hand, and tap the syringe with your other hand. The bubbles will float to the top. Push the bubbles back into the ... To fill a syringe with two ...

Plant growth responses to polypropylene--biocontainers

USDA-ARS?s Scientific Manuscript database

The influence of bio-fillers incorporated into polypropylene (PP) on the growth of plants was evaluated. Biocontainers were created by injection molding of PP with 25-40% by weight of Osage orange tree, Paulownia tree, coffee tree wood or dried distillers grain and 5% by weight of maleated polypropy...

Freeze-dried spermatozoa: A future tool?

PubMed

Olaciregui, M; Gil, L

2017-04-01

Cryopreservation has been routinely used to preserve sperm of human and different animal species. However, frozen sperm storage for a long time brings many inconveniences because of liquid nitrogen. Many attempts have been made to overcome the disadvantages of the current cryopreservation method. Freeze-drying has been proposed as alternative method for sperm preservation to achieve the ability to store sperm doses indefinitely at ambient temperature or in ordinary refrigerators. At present, it has been reported successfully sperm freeze-drying on many animal species including canine and feline. It is well known that during freeze-drying process, sperm DNA could be damaged, but if suitable protection is provided, the sperm nucleus could preserve the ability to activate the oocyte and embryos could be generated by intracytoplasmic sperm injection (ICSI). Many factors influence the freeze-drying efficacy, so current researches have been conducted to find strategies to control these factors to maintain the sperm DNA integrity. This review describes the latest method of sperm freeze-drying for practical application in preserving and transporting genetic resources. In addition, the approaches to improve the efficiency of the technique were studied. We demonstrated that the DNA integrity of freeze-dried dog sperm is affected by the composition of the freeze-drying solution as well as the temperature and period of storage. Further studies are necessary to refine freeze-drying protocol in order to protect the DNA and maintain the sperm functionality and obtain offspring from freeze-dried sperm. © 2016 Blackwell Verlag GmbH.

Botulinum toxin type A for the treatment of sialorrhoea in amyotrophic lateral sclerosis: a clinical and neurophysiological study.

PubMed

Gilio, Francesca; Iacovelli, Elisa; Frasca, Vittorio; Gabriele, Maria; Giacomelli, Elena; Picchiori, Floriana; Soldo, Pietro; Cipriani, Anna Maria; Ruoppolo, Giovanni; Inghilleri, Maurizio

2010-08-01

Botulinum toxin type A (BoNT/A) has been proposed as an alternative treatment for sialorrhoea in patients with amyotrophic lateral sclerosis (ALS). In an open-label prospective study, BoNT/A was injected into the parotid glands bilaterally using anatomic landmarks in 26 ALS patients with bulbar symptoms. Two weeks after injection the severity of sialorrhoea and the related disability were evaluated subjectively and objectively. A group of healthy subjects acted as controls for saliva production. Patients also underwent electrophysiological tests to evaluate possible toxin effects in the nearby non-injected muscles by comparing the amplitude of compound motor action potentials (cMAPs) elicited by electrical stimulation and recorded from the orbicularis oculi and masseter muscles. After BoNT/A injections, of the 26 patients treated, 23 reported that the severity of sialorrhoea improved and the disabling symptoms diminished. Cotton roll weight also decreased after BoNT/A injection, suggesting a reduction in saliva production. Two patients complained of dry mouth. BoNT/A injection left the cMAP amplitude unchanged, suggesting that botulinum toxin does not significantly affect the non-injected facial and masticatory muscles. In conclusion, intraparotid anatomically-guided BoNT/A injection is an effective, easy, and safe treatment for sialorrhoea in patients with bulbar symptoms related to ALS.

The mercury levels in crustaceans and cephalopods from Peninsular Malaysia.

PubMed

Ahmad, Nurul Izzah; Noh, Mohd Fairulnizal Mohd; Mahiyuddin, Wan Rozita Wan; Jaafar, Hamdan; Ishak, Ismail; Azmi, Wan Nurul Farah Wan; Veloo, Yuvaneswary; Mokhtar, Fazlin Anis

2015-09-01

This study is to determine total mercury in edible tissues of eight species of cephalopods and 12 species of crustaceans purchased from 11 identified major fish landing ports and wet markets throughout Peninsular Malaysia. The concentration of mercury was measured by cold vapor atomic absorption spectrometry (AAS) technique using the Perkin Elmer Flow Injection Mercury System (FIMS-400). In general, the mercury levels were low with concentrations in cephalopods ranging from 0.099 to 2.715 mg/kg dry weight (or 0.0184-0.505 mg/kg wet weight) and in crustaceans ranging from 0.057 to 1.359 mg/kg dry weight (or 0.0111-0.265 mg/kg wet weight). The mercury levels showed no significant differences (P > 0.05) between species for both cephalopods and crustaceans. There was no significant correlation between mercury concentrations and the body size of individual for both groups as well. Comparisons with mercury levels obtained found from other previous studies and/or species noted that they were of the same magnitude or relatively low compared to various locations reported worldwide.

Real-time monitoring of fragrance release from cotton towels by low thermal mass gas chromatography using a longitudinally modulating cryogenic system for headspace sampling and injection.

PubMed

Haefliger, Olivier P; Jeckelmann, Nicolas; Ouali, Lahoussine; León, Géraldine

2010-01-15

An innovative headspace sampling and injection system for gas chromatography was designed using a longitudinally modulating cryogenic system mounted around the sampling loop of a two-position loop injector. The setup was hyphenated to a fast low thermal mass gas chromatograph, allowing transient concentrations of semivolatile analytes to be monitored in real time with a time resolution of 4.5 min. The performance of the instrument, and in particular its cryotrapping efficiency, was characterized using a mixture of long-chain alkanes, methyl esters, ethyl esters, and alcohols of different volatilities. The device was found to be ideally suited to the analysis of semivolatile compounds with boiling points ranging between 190 and 320 degrees C, which are typical for a majority of perfumery raw materials. The new instrument was successfully used to monitor the release of eight odorant compounds from cotton towels to which fabric softener had been applied that alternatively contained the fragrance in free form or in microencapsulated form. The analytical results, unprecedented in their level of precision and time resolution for such an application, evidenced the major impact of microencapsulation technology on the kinetics of fragrance release during the drying of the towels and on the triggering of additional fragrance release by applying mechanical stress to the fabric to rupture the microcapsule walls.

The Many Problems with Geoengineering Using Stratospheric Aerosols

NASA Astrophysics Data System (ADS)

Robock, Alan

2009-05-01

In response to the global warming problem, there has been a recent renewed call for geoengineering ``solutions'' involving injecting particles into the stratosphere or blocking sunlight with satellites between the Sun and Earth. While volcanic eruptions have been suggested as innocuous examples of stratospheric aerosols cooling the planet, the volcano analog actually argues against geoengineering because of ozone depletion and regional hydrologic and temperature responses. In this talk, I consider the suggestion to create an artificial stratospheric aerosol layer. No systems to conduct geoengineering now exist, but a comparison of different proposed stratospheric injection schemes, airplanes, balloons, artillery, and a space elevator, shows that using airplanes would not be that expensive. We simulated the climate response to both tropical and Arctic stratospheric injection of sulfate aerosol precursors using a comprehensive atmosphere-ocean general circulation model, the National Aeronautics and Space Administration Goddard Institute for Space Studies ModelE. We simulated the injection of SO2 and the model converts it to sulfate aerosols, transports them and removes them through dry and wet deposition, and calculates the climate response to the radiative forcing from the aerosols. We conducted simulations of future climate with the Intergovernmental Panel on Climate Change A1B business-as-usual scenario both with and without geoengineering, and compare the results. We found that if there were a way to continuously inject SO2 into the lower stratosphere, it would produce global cooling. Acid deposition from the sulfate would not be enough to disturb most ecosystems. Tropical SO2 injection would produce sustained cooling over most of the world, with more cooling over continents. Arctic SO2 injection would not just cool the Arctic. But both tropical and Arctic SO2 injection would disrupt the Asian and African summer monsoons, reducing precipitation to the food supply for billions of people. These regional climate anomalies are but one of many reasons why geoengineering may be a bad idea. I also discuss 19 other reasons. Global efforts to mitigate anthropogenic emissions and to adapt to climate change are a much better way to channel our resources to address anthropogenic global warming.

Dry sorbent injection of trona to control acid gases from a pilot-scale coal-fired combustion facility

EPA Science Inventory

Gaseous and particulate emissions from the combustion of coal have been associated with adverse effects on human and environmental health, and have for that reason been subject to regulation by federal and state governments. Recent regulations by the United States Environmental ...

EVALUATION OF FGD DRY INJECTION SORBENTS AND ADDITIVES - VOLUME 1 - DEVELOPMENT OF HIGH REACTIVITY SORBENTS

EPA Science Inventory

The report discusses recent work addressing lime enhancement by slurrying with siliceous materials and testing in a laboratory packed-bed reactor, as part of EPA's efforts to develop low cost, retrofit flue gas cleaning technology, including the development of highly reactive sor...

Performance of high lignin content cellulose nanocrystals in poly(lactic acid)

Treesearch

Liqing Wei; Umesh P. Agarwal; Laurent Matuana; Ronald C. Sabo; Nicole M. Stark

2018-01-01

High lignin-containing cellulose nanocrystals (HLCNCs) were successfully isolated from hydrothermally treated aspen fibers and freeze-dried and compounded with poly (lactic acid) (PLA) by extrusion and injection molding. As a comparison, PLA composites containing commercial lignin-coated CNCs (BLCNCs) were also produced. HLCNCs showed higher crystallinity, larger...

30 CFR 75.1101-22 - Inspection of dry powder chemical systems.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Inspection of dry powder chemical systems. 75...-22 Inspection of dry powder chemical systems. (a) Each dry powder chemical system shall be examined... the dry powder chemical system has been actuated, all components of the system shall be cleaned...

30 CFR 75.1101-22 - Inspection of dry powder chemical systems.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Inspection of dry powder chemical systems. 75...-22 Inspection of dry powder chemical systems. (a) Each dry powder chemical system shall be examined... the dry powder chemical system has been actuated, all components of the system shall be cleaned...

30 CFR 75.1101-22 - Inspection of dry powder chemical systems.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Inspection of dry powder chemical systems. 75...-22 Inspection of dry powder chemical systems. (a) Each dry powder chemical system shall be examined... the dry powder chemical system has been actuated, all components of the system shall be cleaned...

30 CFR 75.1101-22 - Inspection of dry powder chemical systems.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Inspection of dry powder chemical systems. 75...-22 Inspection of dry powder chemical systems. (a) Each dry powder chemical system shall be examined... the dry powder chemical system has been actuated, all components of the system shall be cleaned...

Effects of silk fibroin in murine dry eye

NASA Astrophysics Data System (ADS)

Kim, Chae Eun; Lee, Ji Hyun; Yeon, Yeung Kyu; Park, Chan Hum; Yang, Jaewook

2017-03-01

The study aimed to investigate the effects of silk fibroin in a mouse model of dry eye. The experimental dry eye mouse model was developed using more than twelve-weeks-old NOD.B10.H2b mice exposing them to 30-40% ambient humidity and injecting them with scopolamine hydrobromide for 10 days. Tear production and corneal irregularity score were measured by the instillation of phosphate buffered saline or silk fibroin. Corneal detachment and conjunctival goblet cell density were observed by hematoxylin and eosin or periodic acid Schiff staining in the cornea or conjunctiva. The expression of inflammatory markers was detected by immunohistochemistry in the lacrimal gland. The silk group tear production was increased, and corneal smoothness was improved. The corneal epithelial cells and conjunctival goblet cells were recovered in the silk groups. The expression of inflammatory factors was inhibited in the lacrimal gland of the silk group. These results show that silk fibroin improved the cornea, conjunctiva, and lacrimal gland in the mouse model of dry eye. These findings suggest that silk fibroin has anti-inflammatory effects in the experimental models of dry eye.

Complete denture analyzed by optical coherence tomography

NASA Astrophysics Data System (ADS)

Negrutiu, Meda L.; Sinescu, Cosmin; Todea, Carmen; Podoleanu, Adrian G.

2008-02-01

The complete dentures are currently made using different technologies. In order to avoid deficiencies of the prostheses made using the classical technique, several alternative systems and procedures were imagined, directly related to the material used and also to the manufacturing technology. Thus, at the present time, there are several injecting systems and technologies on the market, that use chemoplastic materials, which are heat cured (90-100°C), in dry or wet environment, or cold cured (below 60°C). There are also technologies that plasticize a hard cured material by thermoplastic processing (without any chemical changes) and then inject it into a mold. The purpose of this study was to analyze the existence of possible defects in several dental prostheses using a non invasive method, before their insertion in the mouth. Different dental prostheses, fabricated from various materials were investigated using en-face optical coherence tomography. In order to discover the defects, the scanning was made in three planes, obtaining images at different depths, from 0,01 μm to 2 mm. In several of the investigated prostheses we found defects which may cause their fracture. These defects are totally included in the prostheses material and can not be vizualised with other imagistic methods. In conclusion, en-face OCT is an important investigative tool for the dental practice.

Sustainable in-well vapor stripping: A design, analytical model, and pilot study for groundwater remediation.

PubMed

Sutton, Patrick T; Ginn, Timothy R

2014-12-15

A sustainable in-well vapor stripping system is designed as a cost-effective alternative for remediation of shallow chlorinated solvent groundwater plumes. A solar-powered air compressor is used to inject air bubbles into a monitoring well to strip volatile organic compounds from a liquid to vapor phase while simultaneously inducing groundwater circulation around the well screen. An analytical model of the remediation process is developed to estimate contaminant mass flow and removal rates. The model was calibrated based on a one-day pilot study conducted in an existing monitoring well at a former dry cleaning site. According to the model, induced groundwater circulation at the study site increased the contaminant mass flow rate into the well by approximately two orders of magnitude relative to ambient conditions. Modeled estimates for 5h of pulsed air injection per day at the pilot study site indicated that the average effluent concentrations of dissolved tetrachloroethylene and trichloroethylene can be reduced by over 90% relative to the ambient concentrations. The results indicate that the system could be used cost-effectively as either a single- or multi-well point technology to substantially reduce the mass of dissolved chlorinated solvents in groundwater. Copyright © 2014 Elsevier B.V. All rights reserved.

46 CFR 154.1140 - Dry chemical system: General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Dry chemical system: General. 154.1140 Section 154.1140... Firefighting System: Dry Chemical § 154.1140 Dry chemical system: General. Each liquefied flammable gas carrier must have a dry chemical firefighting system that meets §§ 154.1145 through 154.1170, Part 56 and...

46 CFR 154.1140 - Dry chemical system: General.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Dry chemical system: General. 154.1140 Section 154.1140... Firefighting System: Dry Chemical § 154.1140 Dry chemical system: General. Each liquefied flammable gas carrier must have a dry chemical firefighting system that meets §§ 154.1145 through 154.1170, Part 56 and...

46 CFR 154.1140 - Dry chemical system: General.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Dry chemical system: General. 154.1140 Section 154.1140... Firefighting System: Dry Chemical § 154.1140 Dry chemical system: General. Each liquefied flammable gas carrier must have a dry chemical firefighting system that meets §§ 154.1145 through 154.1170, Part 56 and...

46 CFR 154.1140 - Dry chemical system: General.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Dry chemical system: General. 154.1140 Section 154.1140... Firefighting System: Dry Chemical § 154.1140 Dry chemical system: General. Each liquefied flammable gas carrier must have a dry chemical firefighting system that meets §§ 154.1145 through 154.1170, Part 56 and...

46 CFR 154.1140 - Dry chemical system: General.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Dry chemical system: General. 154.1140 Section 154.1140... Firefighting System: Dry Chemical § 154.1140 Dry chemical system: General. Each liquefied flammable gas carrier must have a dry chemical firefighting system that meets §§ 154.1145 through 154.1170, Part 56 and...

30 CFR 75.1101-14 - Installation of dry powder chemical systems.

Code of Federal Regulations, 2014 CFR

2014-07-01

...' Laboratories, Inc., or Factory Mutual Engineering Corp. (c) The components of each dry powder chemical system... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Installation of dry powder chemical systems. 75...-14 Installation of dry powder chemical systems. (a) Self-contained dry powder chemical systems shall...

30 CFR 75.1101-14 - Installation of dry powder chemical systems.

Code of Federal Regulations, 2012 CFR

2012-07-01

...' Laboratories, Inc., or Factory Mutual Engineering Corp. (c) The components of each dry powder chemical system... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Installation of dry powder chemical systems. 75...-14 Installation of dry powder chemical systems. (a) Self-contained dry powder chemical systems shall...

30 CFR 75.1101-14 - Installation of dry powder chemical systems.

Code of Federal Regulations, 2011 CFR

2011-07-01

...' Laboratories, Inc., or Factory Mutual Engineering Corp. (c) The components of each dry powder chemical system... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Installation of dry powder chemical systems. 75...-14 Installation of dry powder chemical systems. (a) Self-contained dry powder chemical systems shall...

30 CFR 75.1101-14 - Installation of dry powder chemical systems.

Code of Federal Regulations, 2010 CFR

2010-07-01

...' Laboratories, Inc., or Factory Mutual Engineering Corp. (c) The components of each dry powder chemical system... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Installation of dry powder chemical systems. 75...-14 Installation of dry powder chemical systems. (a) Self-contained dry powder chemical systems shall...

30 CFR 75.1101-14 - Installation of dry powder chemical systems.

Code of Federal Regulations, 2013 CFR

2013-07-01

...' Laboratories, Inc., or Factory Mutual Engineering Corp. (c) The components of each dry powder chemical system... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Installation of dry powder chemical systems. 75...-14 Installation of dry powder chemical systems. (a) Self-contained dry powder chemical systems shall...

33 CFR 127.609 - Dry chemical systems.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Dry chemical systems. 127.609... Waterfront Facilities Handling Liquefied Natural Gas Firefighting § 127.609 Dry chemical systems. (a) Each marine transfer area for LNG must have a dry chemical system that provides at least two dry chemical...

33 CFR 127.609 - Dry chemical systems.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Dry chemical systems. 127.609... Waterfront Facilities Handling Liquefied Natural Gas Firefighting § 127.609 Dry chemical systems. (a) Each marine transfer area for LNG must have a dry chemical system that provides at least two dry chemical...

33 CFR 127.609 - Dry chemical systems.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Dry chemical systems. 127.609... Waterfront Facilities Handling Liquefied Natural Gas Firefighting § 127.609 Dry chemical systems. (a) Each marine transfer area for LNG must have a dry chemical system that provides at least two dry chemical...

33 CFR 127.609 - Dry chemical systems.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Dry chemical systems. 127.609... Waterfront Facilities Handling Liquefied Natural Gas Firefighting § 127.609 Dry chemical systems. (a) Each marine transfer area for LNG must have a dry chemical system that provides at least two dry chemical...

33 CFR 127.609 - Dry chemical systems.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Dry chemical systems. 127.609... Waterfront Facilities Handling Liquefied Natural Gas Firefighting § 127.609 Dry chemical systems. (a) Each marine transfer area for LNG must have a dry chemical system that provides at least two dry chemical...

Investigation into the Mechanism of Polymer Thread Drag Reduction

DTIC Science & Technology

1990-01-01

They conducted experiments in a 3.75 cm diameter pipe, Re = 85,000, where they injected drag reducing solutions of guar gum and polyacrylamide, P-295 a...manufactured by Dow Chemical. Concentrations of 5000 ppm and 466 ppm based on weight were used in the experiments. The dry powder was suspended in 300

[Aneurysm in the aorta. Diagnostic confirmation by means of a helicoidal CAT scan].

PubMed

Lema Garrido, Fernando; Moya Merino, Mariano; Jordán López, Cristóbal

2004-09-01

A helicoidal radiological CAT Scan, which produces reconstructions in varying planes and makes use of an injected contrasting liquid, confirms a diagnosis of an abdominal aorta aneurysm in the shortest time possible, whether this aneurysm is broken or dried, in order to facilitate a patient's treatment to the highest degree.

Nanocomposites from lignin-containing cellulose nanocrystals and poly(lactic acid)

Treesearch

Liqing Wei; Umesh Agarwal; Nicole Stark; Ronald Sabo

2017-01-01

Utilizing lignin-containing cellulose nanocrystals (HLCNCs) as reinforcing agents to poly(lactic acid) (PLA) for nanocomposites was studied for the first time. The PLA/HLCNCs nanocomposites were prepared by extrusion and injecting molding. The freeze-dried HLCNCs showed micron scale agglomerates. As indicated by the water contact angle measurements, the HLCNCs were...

30 CFR 250.527 - What must I include in my casing pressure request?

Code of Federal Regulations, 2011 CFR

2011-07-01

... casing/riser calculated MAWOPs; (h) All casing/riser pre-bleed down pressures; (i) Shut-in tubing... test (oil, gas, basic sediment, and water); (l) Well status (shut-in, temporarily abandoned, producing, injecting, or gas lift); (m) Well type (dry tree, hybrid, or subsea); (n) Date of diagnostic test; (o) Well...

Morphological and Crystalline Transitions in Monohydrous and Anhydrous Aripiprazole for a Long-Acting Injectable Suspension.

PubMed

Tan, Xinyi; Zhong, Yue; He, Luying; Zhang, Yuanyuan; Jing, Guanghui; Li, Song; Wang, Jing; He, Haibing; Tang, Xing

2017-05-01

Many formulation and manufacturing processes can lead to morphological and crystalline transitions in many polycrystalline drugs, changing the properties of active pharmaceutical ingredients (APIs) such as solubility and physical stability which influence their therapeutic effects and safety and so limit their usefulness. Here, we report significant changes in crystal forms and morphology, including the shape and size of particles during the manufacture of off-white aripiprazole (APZ) dry powders used for long-acting and injectable suspensions. With the optimal top-down approach, powders were prepared by recrystallizing uniform monohydrous APZ (MA) and polycrystalline anhydrous APZ (AA) form III, characterized by thermal analysis, PXRD, and FT-IR. However, powders involving MA (MAP) with a lower mean size (2.126 μm), narrower distribution (span = 1.90), and higher stability compared with AA dry powders (AAP) were found to exhibit dehydration behavior and morphological changes after completion of the preparation processes based on the results of thermal analysis. In the case of APZ powders, we wished to obtain more information to guide in the industrial production and experimental design of suspensions in the future.

Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome.

PubMed

Meng, Fan-You; Gao, Wei; Ju, Ying-Nan

2017-03-29

Cyclooxygenase-2 (COX-2) contributes to ventilation induced lung injury (VILI) and acute respiratory distress syndrome (ARDS). The objective of present study was to observe the therapeutic effect of parecoxib on VILI in ARDS. In this parallel controlled study performed at Harbin Medical University, China between January 2016 and March 2016, 24 rats were randomly allocated into sham group (S), volume ventilation group/ARDS (VA), parecoxib/volume ventilation group/ARDS (PVA). Rats in the S group only received anesthesia; rats in the VA and PVA group received intravenous injection of endotoxin to induce ARDS, and then received ventilation. Rats in the VA and PVA groups were treated with intravenous injection of saline or parecoxib. The ratio of arterial oxygen pressure to fractional inspired oxygen (PaO 2 /FiO 2 ), the wet to dry weight ratio of lung tissue, inflammatory factors in serum and bronchoalveolar lavage fluid (BALF), and histopathologic analyses of lung tissue were examined. In addition, survival was calculated at 24 h after VILI. Compared to the VA group, in the PVA group, PaO 2 /FiO 2 was significantly increased; lung tissue wet to dry weight ratio; macrophage and neutrophil counts, total protein and neutrophil elastase levels in BALF; tumor necrosis factor-α, interleukin-1β, and prostaglandin E 2 levels in BALF and serum; and myeloperoxidase (MPO) activity, malondialdehyde levels, and Bax and COX-2 protein levels in lung tissue were significantly decreased, while Bcl-2 protein levels were significantly increased. Lung histopathogical changes and apoptosis were reduced by parecpxib in the PVA group. Survival was increased in the PVA group. Parecoxib improves gas exchange and epithelial permeability, decreases edema, reduces local and systemic inflammation, ameliorates lung injury and apoptosis, and increases survival in a rat model of VILI.

Contribution of starter cultures to the proteolytic process of a fermented non-dried whole muscle ham product.

PubMed

Scannell, Amalia G M; Kenneally, Paul M; Arendt, Elke K

2004-06-01

Porcine longissimus dorsi muscles were cured by brine injection. Curing brine containing 15% (w/v) NaCl, 1.33% (w/v) glucose, 750 ppm sodium nitrite, and appropriate levels of either Lactobacillus sakei LAD, L. sakei LAD plus Kocuria varians FT4 (formally Micrococcus varians), L. sakei LAD plus papain and GDL (glucono-delta-lactone) plus K. varians FT4, was injected to the muscle at a pumping rate 15% w/v. The effect of these treatments on the proteolysis in the ham system was compared to a control ham, produced without starter culture and containing GDL acidulant to control pH and antibiotics to reduce the contribution of background microflora. Hydrolysis of sarcoplasmic and myofibrillar protein fractions was evaluated by SDS-PAGE and reverse phase-HPLC. Hams with different treatments were also investigated for differences in amino acid profile, protein and non-protein nitrogen level, colour, pH, water activity and moisture and microbiological evolution. There was no significant difference in the gross compositional analysis of any of the treatments compared to the control. There was no significant difference (p>0.05) in the protein content, non-protein nitrogen level, SDS-PAGE and free amino acid analysis between the control ham and ham inoculated with proteolytic starter culture. However, it was observed that hams containing starter cultures exhibited decreases in certain peptide fractions and corresponding increases in some free amino acids compared to the uninoculated control. It can be concluded that, while the principle mechanisms resulting in the proteolysis of this non-dried ham product involve the activity of endogeneous cathepsins, the addition of proteolytic starter cultures influence the amino acid profile thereby potentially enhancing the sensorial attributes of the ham. Copyright 2004 Elsevier B.V.

Adsorption of mercury by activated carbon prepared from dried sewage sludge in simulated flue gas.

PubMed

Park, Jeongmin; Lee, Sang-Sup

2018-04-25

Conversion of sewage sludge to activated carbon is attractive as an alternative method to ocean dumping for the disposal of sewage sludge. Injection of activated carbon upstream of particulate matter control devices has been suggested as a method to remove elemental mercury from flue gas. Activated carbon was prepared using various activation temperatures and times and was tested for their mercury adsorption efficiency using lab-scale systems. To understand the effect of the physical property of the activated carbon, its mercury adsorption efficiency was investigated as a function of their Brunauer-Emmett-Teller (BET) surface area. Two simulated flue gas conditions: (1) without hydrogen chloride (HCl) and (2) with 20 ppm HCl, were used to investigate the effect of flue gas composition on the mercury adsorption capacity of activated carbon. Despite very low BET surface area of the prepared sewage sludge activated carbons, their mercury adsorption efficiencies were comparable under both simulated flue gas conditions to those of pinewood and coal activated carbons. After injecting HCl into the simulated flue gas, all sewage sludge activated carbons demonstrated high adsorption efficiencies, i.e., more than 87%, regardless of their BET surface area. IMPLICATIONS We tested activated carbons prepared from dried sewage sludge to investigate the effect of their physical properties on their mercury adsorption efficiency. Using two simulated flue gas conditions, we conducted mercury speciation for the outlet gas. We found that the sewage sludge activated carbon had comparable mercury adsorption efficiency to pinewood and coal activated carbons, and the presence of HCl minimized the effect of physical property of the activated carbon on its mercury adsorption efficiency.

Small-scale electrical resistivity tomography of wet fractured rocks.

PubMed

LaBrecque, Douglas J; Sharpe, Roger; Wood, Thomas; Heath, Gail

2004-01-01

This paper describes a series of experiments that tested the ability of the electrical resistivity tomography (ERT) method to locate correctly wet and dry fractures in a meso-scale model. The goal was to develop a method of monitoring the flow of water through a fractured rock matrix. The model was a four by six array of limestone blocks equipped with 28 stainless steel electrodes. Dry fractures were created by placing pieces of vinyl between one or more blocks. Wet fractures were created by injecting tap water into a joint between blocks. In electrical terms, the dry fractures are resistive and the wet fractures are conductive. The quantities measured by the ERT system are current and voltage around the outside edge of the model. The raw ERT data were translated to resistivity values inside the model using a three-dimensional Occam's inversion routine. This routine was one of the key components of ERT being tested. The model presented several challenges. First, the resistivity of both the blocks and the joints was highly variable. Second, the resistive targets introduced extreme changes the software could not precisely quantify. Third, the abrupt changes inherent in a fracture system were contrary to the smoothly varying changes expected by the Occam's inversion routine. Fourth, the response of the conductive fractures was small compared to the background variability. In general, ERT was able to locate correctly resistive fractures. Problems occurred, however, when the resistive fracture was near the edges of the model or when multiple fractures were close together. In particular, ERT tended to position the fracture closer to the model center than its true location. Conductive fractures yielded much smaller responses than the resistive case. A difference-inversion method was able to correctly locate these targets.

Cost analysis of an ammonia dry cooling system with a Chicago Bridge and Iron peak shaving system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Drost, M.K.; Johnson, B.M.

1980-12-01

A study was performed to determine the potential for reducing the cost associated with dry cooling by using an ammonia dry cooling system augmented with the Chicago Bridge and Iron (CP and I) peak shaving system. The cost analysis of an all-dry ammonia cooling system operating in conjunction with a peak shaving system is documented. The peak shaving system utilizes the excess cooling capability available at night to cool water to be used for supplemental cooling during the following day. The analysis consisted of determining the incremental cost of cooling for the CB and I system and comparing this costmore » to the incremental cost of cooling for both dry and wet/dry systems for a consistent set of design conditions and assumptions. The wet/dry systems were analyzed over a range of water usages. The basis of the comparisons was a cooling system designed for installations with a 650 mWe (gross) coal-fired power plant. From results of the study it was concluded that: the CB and I system shows a substantial economic advantage when compared with an all-dry cooling system; the CB and I system appears to be competitive with wet/dry cooling systems using about 2 to 3% water; and the CB and I system demonstrates a clear economic advantage when compared to both dry and wet/dry concepts for a winter peaking utility where the excess generation is assumed to displace both base-loaded coal-fired power generation and oil-fired gas turbine peaking units.« less

Delineating Fecal Contaminant Sources and Travel Times in a Karst Groundwater Basin, Inner Bluegrass Region, Kentucky

NASA Astrophysics Data System (ADS)

Ward, J. W.; Reed, T. M.; Fryar, A. E.; Brion, G. M.

2006-12-01

Because of preferential flowpaths via features such as sinkholes and conduits, karst aquifers are susceptible to non-point-source pollution from agricultural and urban drainage. With many karst aquifers being drinking- water sources, pathogens are contaminants of public health concern. Monitoring of microbial parameters (total coliforms [TC], atypical colonies [AC] and fecal coliform bacteria [FC]) transpired biweekly from December 2002 March 2004 and weekly from February October 2005 at Blue Hole Spring, which drains outlying farm lands and the town of Versailles in the Inner Bluegrass Region of Kentucky. Physicochemical parameters (discharge, temperature, specific conductance, and pH) were measured continuously during the entire period. The AC/TC ratio, which had been employed only in surface water-quality studies, was used with FC counts, precipitation and discharge data to determine sources of fecal loading to ground water as result of land-use practices. An AC/TC ratio < 10 demonstrates fresh input of fecal matter, while a larger ratio can represent a variety of occurrences, including aged fecal material input and/or lack of nutrient input into the system. AC/TC ratio data in the 2002 04 dataset behaved similarly to surface waters, with ratios > 10 during dry periods and < 10 during wet periods, while the 2005 data demonstrated a very irregular pattern. The difference in these two data sets indicated a compositional change within the groundwater basin between the two sampling periods, perhaps as a result of construction at a sewage treatment plant adjoining the spring. Solute (rhodamine WT fluorescent dye and bromide) and particle (1-μm diameter fluorescent latex microspheres) tracer tests were conducted during summer 2006 to examine contaminant mobility within the system under base-flow and storm-flow conditions. Rainfall was limited prior to the base-flow trace, totaling 0.025 cm within 2 weeks prior to the slug injection. Base-flow discharge averaged 400 m3/s and solute breakthrough began ~ 7.5 hours post injection and cleared the system after 77 hours. For the storm-flow trace, rainfall totaled 3.12 cm prior to injection, with another 9.35 cm of rainfall occurring over the two week monitoring period. Spring discharge during the storm-flow trace averaged 0.443 m3/s, with a maximum of 0.503 m3/s. Under storm-flow conditions solute breakthrough began ~ 2.33 hours post injection, with particle breakthrough beginning ~ 2.5 hours post injection. Bromide concentrations at the spring were < 0.1 ppm (the detection limit, or DL) 5.5 hours after injection, while rhodamine WT concentrations were < DL (0.1 ppb) 14 hours post injection. Microspheres were detected at the spring until 164 hours after injection. These traces demonstrate that storms in this karst basin can accelerate solute movement, and particles can remain mobile for as long as 1 week after introduction.

[A study of linearity and reciprocity during shock applied with a hammer to human dry skull].

PubMed

Kumazawa, Y; Sekiguchi, J; Saito, M; Honma, K; Toyoda, M; Matsuo, E

1990-09-01

The authors used a human dry skull on which the cranial bone mandible had been joined with an artificial articulator disk to form a single unit. Impact acceleration corresponding to weak and strong tapping was considered a dynamic load in examining the vibration transfer characteristics of the facial cranial bone when impact was applied from the mentum section in a situation designed to be closer to reality. Flexion injection type (resonance frequency f0 = 100 to 150 Hz, produced by GC Corp.) was applied to the human dry skull as an artificial periodontal membrane at thickness of 0.3 mm. In addition, Exaflex heavy body type (f0 = 400 Hz, produced by GC Corp.) was applied as an artificial disk. This was then placed on a damper produced by spreading a rubber dam sheet with a thickness of 35 microns on a tire tube with a diameter of 35 cm and an air pressure of 35 kg/cm2. Investigations were then made concerning linearity and reciprocity to determine whether an experimental system could be achieved or not. This was then followed by modal analysis. As a result, the following matters were ascertained: (1) The resonating area differed according to the extent of the force. (2) An increase in the viscoelastic elements of the silicon was accompanied by attenuation of force. (3) Directionality of force attenuation was caused by the complexity of bone structure. (4) A tapping force of 0.3G or 1G was sufficiently attenuated by the facial cranial bone. (5) The transfer function at the bone seams and thinner areas of the bones was insufficient for modal analysis of the facial region and total cranial bone of the human dry skull.

Hot Dry Rock Geothermal Energy Development Program. Annual report, fiscal year 1979

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cremer, G.M.; Duffield, R.B.; Smith, M.C.

1980-08-01

The Fenton Hill Project is still the principal center for developing methods, equipment, and instrumentation for creating and utilizing HDR geothermal reservoirs. The search for a second site for a similar experimental system in a different geological environment has been intensified, as have the identification and characterization of other HDR areas that may prove suitable for either experimental or commercial development. The Phase I fracture system was enlarged during FY79. Drilling of the injection well of the Phase II system began at Fenton Hill in April 1979. Environmental monitoring of the Fenton Hill area continued through FY79. The environmental studiesmore » indicate that the hot dry rock operations have caused no significant environmental impact. Other supporting activities included rock physics, rock mechanics, fracture mapping, and instrumentation development. Two closely related activities - evaluation of the potential HDR energy resource of the US and the selection of a site for development of a second experimental heat-extraction system generally similar to that at Fenton Hill - have resulted in the collection of geology, hydrology, and heat-flow data on some level of field activity in 30 states. The resource-evaluation activity included reconnaissance field studies and a listing and preliminary characterization of US geothermal areas in which HDR energy extraction methods may be applicable. The selection of Site 2 has taken into account such legal, institutional, and economic factors as land ownership and use, proximity to possible users, permitting and licensing requirements and procedures, environmental issues, areal extent of the geothermal area, and visibility to and apparent interest by potential industrial developers.« less

Hydroxypropyl-ß-cyclodextrin as a membrane protectant during freeze-drying of hydrogenated and non-hydrogenated liposomes and molecule-in-cyclodextrin-in- liposomes: Application to trans-anethole.

PubMed

Gharib, Riham; Greige-Gerges, Hélène; Fourmentin, Sophie; Charcosset, Catherine

2018-11-30

The effect of hydrogenation of phospholipids on the characteristics of freeze-dried liposomes was investigated using hydroxypropyl-ß-cyclodextrin (HP-ß-CD) as membrane protectant. The ethanol-injection method was applied to prepare liposomes using hydrogenated (Phospholopion-90H and 80H) and non-hydrogenated phospholipids (Lipoid-S100) in combination with cholesterol. Various liposomal formulations were tested: conventional liposomes (CL) and HP-ß-CD-loaded liposomes (CDL). Liposome suspensions were concentrated by ultracentrifugation; the pellets were reconstituted in water or CD solution and the dispersions were characterized for their size, polydispersity index and zeta potential. Results demonstrated that HP-ß-CD protected only the hydrogenated batches (CL and CDL) during freeze-drying. Moreover, the presence of HP-ß-CD in the aqueous phase of CDL protected them during freeze-drying. Freeze-dried CL and CDL made of phospholipon-90H loading anethole were demonstrated to be physically stable upon reconstitution in HP-ß-CD solutions, and are able to retain anethole after 6 months of storage at 4 °C thereby making them valuable for food applications. Copyright © 2017 Elsevier Ltd. All rights reserved.

Pressure evolution and deformation of confined granular media during pneumatic fracturing

NASA Astrophysics Data System (ADS)

Eriksen, Fredrik K.; Toussaint, Renaud; Turquet, Antoine Léo; Mâløy, Knut J.; Flekkøy, Eirik G.

2018-01-01

By means of digital image correlation, we experimentally characterize the deformation of a dry granular medium confined inside a Hele-Shaw cell due to air injection at a constant overpressure high enough to deform it (from 50 to 250 kPa). Air injection at these overpressures leads to the formation of so-called pneumatic fractures, i.e., channels empty of beads, and we discuss the typical deformations of the medium surrounding these structures. In addition we simulate the diffusion of the fluid overpressure into the medium, comparing it with the Laplacian solution over time and relating pressure gradients with corresponding granular displacements. In the compacting medium we show that the diffusing pressure field becomes similar to the Laplace solution on the order of a characteristic time given by the properties of the pore fluid, the granular medium, and the system size. However, before the diffusing pressure approaches the Laplace solution on the system scale, we find that it resembles the Laplacian field near the channels, with the highest pressure gradients on the most advanced channel tips and a screened pressure gradient behind them. We show that the granular displacements more or less always move in the direction against the local pressure gradients, and when comparing granular velocities with pressure gradients in the zone ahead of channels, we observe a Bingham type of rheology for the granular paste (the mix of air and beads), with an effective viscosity μB and displacement thresholds ∇ ⃗Pc evolving during mobilization and compaction of the medium. Such a rheology, with disorder in the displacement thresholds, could be responsible for placing the pattern growth at moderate injection pressures in a universality class like the dielectric breakdown model with η =2 , where fractal dimensions are found between 1.5 and 1.6 for the patterns.

Influence of processing steps in cold-smoked salmon production on survival and growth of persistent and presumed non-persistent Listeria monocytogenes.

PubMed

Porsby, Cisse Hedegaard; Vogel, Birte Fonnesbech; Mohr, Mona; Gram, Lone

2008-03-20

Cold-smoked salmon is a ready-to-eat product in which Listeria monocytogenes sometimes can grow to high numbers. The bacterium can colonize the processing environment and it is believed to survive or even grow during the processing steps. The purpose of the present study was to determine if the steps in the processing of cold-smoked salmon affect survival and subsequent growth of a persistent strain of L. monocytogenes to a lesser degree than presumed non-persistent strains. We used a sequence of experiments increasing in complexity: (i) small salmon blocks salted, smoked or dried under model conditions, (ii) fillets of salmon cold-smoked in a pilot plant and finally, (iii) assessment of the bacterial levels before and after processing during commercial scale production. L. monocytogenes proliferated on salmon blocks that were brined or dipped in liquid smoke and left at 25 degrees C in a humidity chamber for 24 h. However, combining brining and liquid smoke with a drying (25 degrees C) step reduced the bacterium 10-100 fold over a 24 h period. Non-salted, brine injected or dry salted salmon fillets were surface inoculated with L. monocytogenes and cold-smoked in a pilot plant. L. monocytogenes was reduced from 10(3) to 10-10(2) CFU/cm(2) immediately after cold-smoking. The greatest reductions were observed in dry salted and brine injected fillets as compared to cold-smoking of non-salted fresh fillets. Levels of L. monocytogenes decreased further when the cold-smoked fish was vacuum-packed and stored at 5 degrees C. A similar decline was seen when inoculating brine injected fillets after cold-smoking. High phenol concentrations are a likely cause of this marked growth inhibition. In a commercial production facility, the total viable count of salmon fillets was reduced 10-1000 fold by salting, cold-smoking and process-freezing (a freezing step after smoking and before slicing). The prevalence of L. monocytogenes in the commercial production facility was too low to determine any quantitative effects, however, one of nine samples was positive before processing and none after. Taken together, the processing steps involved in cold-smoking of salmon are bactericidal and reduce, but do not eliminate L. monocytogenes. A persistent strain was no less sensitive to the processing steps than a clinical strain or strain EGD.

Inorganic N and P dynamics of Antarctic glacial meltwater streams as controlled by hyporheic exchange and benthic autotrophic communities

USGS Publications Warehouse

McKnight, Diane M.; Runkel, R.L.; Tate, C.M.; Duff, J.H.; Moorhead, D.L.

2004-01-01

The McMurdo Dry Valleys of South Victoria Land, Antarctica, contain numerous glacial meltwater streams that drain into lakes on the valley floors. Many of the streams have abundant perennial mats of filamentous cyanobacteria. The algal mats grow during streamflow in the austral summer and are in a dormant freeze-dried state during the rest of the year. NO3 and soluble reactive P (SRP) concentrations were lower in streams with abundant algal mats than in streams with sparse algal mats. NO3 and SRP concentrations were higher in the hyporheic zone of a stream with abundant algal mats than in the stream itself. An experimental injection of LiCl, NaNO3, and K3PO4 was conducted in Green Creek, which has abundant algal mats. Substantial hyporheic exchange occurred. The NO3 and PO4 concentrations at 50 m below the injection were 55 ??M and 18 ??M, respectively, during the experiment. NO3 and PO4 concentrations were below the detection limit of 1 to 2 ??M at a site 497 m below the injection during the Cl tracer arrival, indicating a high capacity for nutrient uptake by algal communities. NO2 and NH4 were present at sites 226 and 327 m below the injection, indicating that, in addition to denitrification and algal uptake, dissimilatory NO3 reduction to NO2 and NH4 may be a NO3 sink during transport. Transport modelling with nutrient uptake represented as a 1st-order process yielded reach-scale parameters of 4.3 ?? 10-5 to 3.9 ?? 10-4/s and 1.4 ?? 10-4 to 3.8 ?? 10 -4/s for uptake of NO3 and PO4, respectively. The best match with the observed data was a model in which PO4 uptake occurred only in the main channel and NO3 uptake occurred in the main channel and in the hyporheic zone. Hyporheic NO3 uptake was 7 to 16% of the total uptake for the different stream reaches. These results demonstrate that nutrient flux to the lakes is controlled by hyporheic exchange and nutrient uptake by algal mats in dry valley streams. Streams without algal mats contribute more nutrients to the lakes than streams with algal mats.

Freeze-dried equine-derived redback spider antivenom: a local irritation study by intramuscular injection in rabbits and a repeated-dose toxicity study in rats.

PubMed

Yamamoto, Akihiko; Harano, Satomi; Shinya, Noriko; Nagano, Ayataka; Miyatsu, Yoshinobu; Sawabe, Kyouko; Matsumura, Takayuki; Ato, Manabu; Takahashi, Motohide; Taki, Hisashi; Hifumi, Toru

2018-04-01

The redback spider ( Latrodectus hasseltii ) is nonindigenous to Japan but has now spread throughout the country. Bites to humans are rare but can be fatal. We prepared freeze-dried redback spider antivenom for therapeutic use against bites in Japan by immunization of horse plasma. This study included two nonclinical tests of the antivenom: a local irritation study involving a single intramuscular administration to rabbits (with injections of physiological saline and an existing freeze-dried diphtheria antitoxin as control and comparison substances, respectively) and a 2-week repeated intermittent intravenous-dose toxicity study in rats. The irritation study showed the antivenom's irritancy to be comparable with that of the saline and the existing antitoxin preparations under the test conditions. In a repeated-dose toxicity study, no toxicity change was found in male or female rats, and the no-observed-adverse-effect level (NOAEL) was judged to be a dose volume of 20 mL/kg (1082 units/kg antivenom activity) in both male and female rats. In addition, there was no toxicological difference between proteinaceous diphtheria antitoxin and redback spider antivenom prepared to have the same protein content and the same additive composition. Based on these findings, we will further advance our research towards clinical application of the redback spider antivenom. This research was supported by the Research Program on Emerging and Re-emerging Infectious Disease of the Japan Agency for Medical Research and Development.

THE COMPARISON OF SEVERAL STANDARD MATERIALS AND TECHNIQUES FOR THE WARREN-AVERBACH DETERMINATION OF MICRO-STRUCTURE CHARACTERISTICS OF CALCIUM HYDROXIDE SORBENT MATERIALS

EPA Science Inventory

The paper gives results of a comparison of several standard materials and techniques for the Warren-Averbach determination of microstructure characteristics of calcium hydroxide--Ca(OH)2--sorbent materials. The comparison is part of an investigation of the injection of dry Ca(OH)...

THE EFFECT OF WATER (VAPOR-PHASE) AND CARBON ON ELEMENTAL MERCURY REMOVAL IN A FLOW REACTOR

EPA Science Inventory

The paper gives results of studying the effect of vapor-phase moisture on elemental mercury (Hgo) removal by activated carbon (AC) in a flow reactor. tests involved injecting AC into both a dry and a 4% moisture nitrogen (N2) /Hgo gas stream. A bituminous-coal-based AC (Calgon WP...

Dehydration of Traditional Dried Instant Noodle (Mee Siput) Using Controlled Temperature & Humidity Dryer

NASA Astrophysics Data System (ADS)

Mamat, K. A.; Yusof, M. S.; Yusoff, Wan Fauziah Wan; Zulafif Rahim, M.; Hassan, S.; Rahman, M. Qusyairi. A.; Karim, M. A. Abd

2017-05-01

Drying process is an essential step to produce instant noodles. Yet, the industries especially Small and Medium Enterprises (SMEs), is seeking for an efficient method to dry the noodles. This paper discusses the performance of an invented drying system which employed heating and humidifying process. The drying system was tested using 30 kilogram of the raw noodle known as “Mee Siput”. Temperature controlled system were used in the study to control the temperature of the drying process and prevent the dried noodles from damage by maintaining the temperature of lower than 80°C. The analysis shows that the system was drastically decreased the humidity from 80% to 40% just after 200 minutes of the drying process. The complete dehydration time of noodle has also decreased to only 4 hours from 16 hours when using traditional drying system without sacrificed the good quality of the dried noodle. In overall, the invented system believed to increase the production capacity of the noodle, reduce cost of production which would highly beneficial for Small Medium Industries (SMEs) in Malaysia.

The celebrated écorchés of Honoré Fragonard, part 1: The classical techniques of preparation of dry anatomical specimens in the 18th century.

PubMed

Degueurce, Christophe; Adds, Philip

2010-04-01

The écorchés that Honoré Fragonard created between 1766 and 1771 have miraculously survived the ravages of time due to a technique of preparation which Fragonard never revealed. The present paper and a subsequent article aim to explain the classical methods used by anatomists of the 18th century (Part 1) and to throw light on the details of Fragonard's method (Part 2). Anatomists of the 18th century who wished to preserve their dissections used a method of mummification, which has now fallen into disuse: drying after immersion in alcohol. This article explains the stages of the classical method utilized by French anatomists of the Age of Enlightenment. The cadaver was selected with care before the vascular system was injected with a colored mixture of wax, animal fat, and plant resins. The body was then dehydrated by immersion in a bath of alcohol, after which it was removed and positioned by means of a wooden framework, which held the body in the desired pose while the alcohol evaporated. The vessels were painted, and finally the body was varnished. 2010 Wiley-Liss, Inc.

Characterization of a high-pressure diesel fuel injection system as a control technology option to improve engine performance and reduce exhaust emissions

NASA Technical Reports Server (NTRS)

Mcfadden, J. J.; Dezelick, R. A.; Barrows, R. R.

1983-01-01

Test results from a high pressure electronically controlled fuel injection system are compared with a commercial mechanical injection system on a single cylinder, diesel test engine using an inlet boost pressure of 2.6:1. The electronic fuel injection system achieved high pressure by means of a fluid intensifier with peak injection pressures of 47 to 69 MPa. Reduced exhaust emissions were demonstrated with an increasing rate of injection followed by a fast cutoff of injection. The reduction in emissions is more responsive to the rate of injection and injection timing than to high peak injection pressure.

29 CFR 1910.161 - Fixed extinguishing systems, dry chemical.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 5 2011-07-01 2011-07-01 false Fixed extinguishing systems, dry chemical. 1910.161 Section... § 1910.161 Fixed extinguishing systems, dry chemical. (a) Scope and application. This section applies to all fixed extinguishing systems, using dry chemical as the extinguishing agent, installed to meet a...

30 CFR 75.1101-13 - Dry powder chemical systems; general.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Dry powder chemical systems; general. 75.1101-13 Section 75.1101-13 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...-13 Dry powder chemical systems; general. Self-contained dry powder chemical systems may be installed...

29 CFR 1910.161 - Fixed extinguishing systems, dry chemical.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 5 2010-07-01 2010-07-01 false Fixed extinguishing systems, dry chemical. 1910.161 Section... § 1910.161 Fixed extinguishing systems, dry chemical. (a) Scope and application. This section applies to all fixed extinguishing systems, using dry chemical as the extinguishing agent, installed to meet a...

30 CFR 75.1101-13 - Dry powder chemical systems; general.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Dry powder chemical systems; general. 75.1101-13 Section 75.1101-13 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...-13 Dry powder chemical systems; general. Self-contained dry powder chemical systems may be installed...

29 CFR 1910.161 - Fixed extinguishing systems, dry chemical.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 5 2013-07-01 2013-07-01 false Fixed extinguishing systems, dry chemical. 1910.161 Section... § 1910.161 Fixed extinguishing systems, dry chemical. (a) Scope and application. This section applies to all fixed extinguishing systems, using dry chemical as the extinguishing agent, installed to meet a...

30 CFR 75.1101-13 - Dry powder chemical systems; general.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Dry powder chemical systems; general. 75.1101-13 Section 75.1101-13 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...-13 Dry powder chemical systems; general. Self-contained dry powder chemical systems may be installed...

29 CFR 1910.161 - Fixed extinguishing systems, dry chemical.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 5 2014-07-01 2014-07-01 false Fixed extinguishing systems, dry chemical. 1910.161 Section... § 1910.161 Fixed extinguishing systems, dry chemical. (a) Scope and application. This section applies to all fixed extinguishing systems, using dry chemical as the extinguishing agent, installed to meet a...

30 CFR 75.1101-13 - Dry powder chemical systems; general.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Dry powder chemical systems; general. 75.1101-13 Section 75.1101-13 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...-13 Dry powder chemical systems; general. Self-contained dry powder chemical systems may be installed...

29 CFR 1910.161 - Fixed extinguishing systems, dry chemical.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 5 2012-07-01 2012-07-01 false Fixed extinguishing systems, dry chemical. 1910.161 Section... § 1910.161 Fixed extinguishing systems, dry chemical. (a) Scope and application. This section applies to all fixed extinguishing systems, using dry chemical as the extinguishing agent, installed to meet a...

30 CFR 75.1101-13 - Dry powder chemical systems; general.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Dry powder chemical systems; general. 75.1101-13 Section 75.1101-13 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...-13 Dry powder chemical systems; general. Self-contained dry powder chemical systems may be installed...

Effects on craniofacial growth and development of unilateral botulinum neurotoxin injection into the masseter muscle.

PubMed

Tsai, Chi-Yang; Chiu, Wan Chi; Liao, Yi-Hsuan; Tsai, Chih-Mong

2009-02-01

The effects of botulinum neurotoxin type A (BoNT/A) on masseter muscles, when injected for cosmetic purposes (volumetric reduction) or treatment of excessive muscle activity (bruxism), have been investigated. However, the full anatomic effects of treatment are not known, particularly with respect to the mandible and relevant anthropometric measurements. The intent of this study was to use unilaterial BoNT/A injections to induce localized masseter atrophy and paresis and then to measure the effects of muscle influence on craniofacial growth and development. Growing male Wistar rats, 30 days old, were studied. The experimental group consisted of 8 rats. One side of the masseter muscle was injected with BoNT/A and the other side of the masseter muscle was injected with saline. The side with BoNT/A belonged to 1 group and the side with saline was the sham group. Three rats without injections was the control. After 45 days, the masseter muscles were dissected and weighed. Dry skulls were prepared, and anthropometric measurements determined. One-way ANOVA showed that the animals maintained their weight in both groups; however, the muscles injected with BoNT/A were smaller than the sham or control muscles. Anthropometric measurements of the bony structures attached to the masseter muscle showed a significant treatment effect. After localized masseter muscle atrophy induced by BoNT/A injection, alterations of craniofacial bone growth and development were seen. The results agree with the functional matrix theory that soft tissues regulate bone growth.

Applying dynamic data collection to improve dry electrode system performance for a P300-based brain-computer interface

NASA Astrophysics Data System (ADS)

Clements, J. M.; Sellers, E. W.; Ryan, D. B.; Caves, K.; Collins, L. M.; Throckmorton, C. S.

2016-12-01

Objective. Dry electrodes have an advantage over gel-based ‘wet’ electrodes by providing quicker set-up time for electroencephalography recording; however, the potentially poorer contact can result in noisier recordings. We examine the impact that this may have on brain-computer interface communication and potential approaches for mitigation. Approach. We present a performance comparison of wet and dry electrodes for use with the P300 speller system in both healthy participants and participants with communication disabilities (ALS and PLS), and investigate the potential for a data-driven dynamic data collection algorithm to compensate for the lower signal-to-noise ratio (SNR) in dry systems. Main results. Performance results from sixteen healthy participants obtained in the standard static data collection environment demonstrate a substantial loss in accuracy with the dry system. Using a dynamic stopping algorithm, performance may have been improved by collecting more data in the dry system for ten healthy participants and eight participants with communication disabilities; however, the algorithm did not fully compensate for the lower SNR of the dry system. An analysis of the wet and dry system recordings revealed that delta and theta frequency band power (0.1-4 Hz and 4-8 Hz, respectively) are consistently higher in dry system recordings across participants, indicating that transient and drift artifacts may be an issue for dry systems. Significance. Using dry electrodes is desirable for reduced set-up time; however, this study demonstrates that online performance is significantly poorer than for wet electrodes for users with and without disabilities. We test a new application of dynamic stopping algorithms to compensate for poorer SNR. Dynamic stopping improved dry system performance; however, further signal processing efforts are likely necessary for full mitigation.

Injection System for Multi-Well Injection Using a Single Pump

PubMed Central

Wovkulich, Karen; Stute, Martin; Protus, Thomas J.; Mailloux, Brian J.; Chillrud, Steven N.

2015-01-01

Many hydrological and geochemical studies rely on data resulting from injection of tracers and chemicals into groundwater wells. The even distribution of liquids to multiple injection points can be challenging or expensive, especially when using multiple pumps. An injection system was designed using one chemical metering pump to evenly distribute the desired influent simultaneously to 15 individual injection points through an injection manifold. The system was constructed with only one metal part contacting the fluid due to the low pH of the injection solutions. The injection manifold system was used during a three-month pilot scale injection experiment at the Vineland Chemical Company Superfund site. During the two injection phases of the experiment (Phase I = 0.27 L/min total flow, Phase II = 0.56 L/min total flow), flow measurements were made 20 times over three months; an even distribution of flow to each injection well was maintained (RSD <4%). This durable system is expandable to at least 16 injection points and should be adaptable to other injection experiments that require distribution of air-stable liquids to multiple injection points with a single pump. PMID:26140014

A new shock wave assisted wood preservative injection system

NASA Astrophysics Data System (ADS)

Rao, K. S.; Ravikumar, G.; Lai, Ram; Jagadeesh, G.

Preservative treatment of many tropical hard woods and bamboo pose severe problem. A number of wood preservatives (chemical formulations toxic to wood decay/ destroying organisms like fungi, wood destroying termites, marine borers etc.) and wood impregnating techniques are currently in use for improving bio resistance of timber and bamboo and thereby enhancing service life for different end uses. How ever, some species of tropical hardwoods and many species of bamboo are difficult to treat, posing technical problems. In this paper we report preliminary results of treatment of bamboo with a novel Shockwave assisted injection treatment. Samples (30×2.5×1.00 cm) of an Indian species of bamboo Dendrocalamus strictus prepared from defect free culms of dry bamboo are placed in the driven section of a vertical shock tube filled with the 4Coppepr-Chrome-Arsenic(CCA) preservative solution.The bamboo samples are subjected to repeated shock wave loading (3 shots) with typical over pressures of 30 bar. The results from the study indicate excellent penetration and retention of CCA preservative in bamboo samples. The method itself is much faster compared to the conventional methods like pressure treatment or hot and cold process.

[A very elderly case of acute-onset autoimmune type 1 diabetes mellitus].

PubMed

Tsuji, Hideyuki

2010-01-01

An 80-year-old man had systemic malaise and pollakiuria, which developed about 40 days before admission. He underwent treatment at a urology department, but his symptoms did not improve. Since dry mouth additionally developed, he visited his family doctor. As his casual blood glucose level was 629 mg/dl and HbA1c was 12.4%, the patient was referred to our department and admitted on the same day. Continuous intravenous infusion of fast-acting insulin and saline were initiated after admission, and dietary therapy at 1,520 kcal/day was initiated on the following day. Anti-GAD antibody and anti-IA-2 antibody were positive, confirming that the disease was acute-onset autoimmune type 1 diabetes mellitus. A sliding scale of fast-acting insulin followed by intensified therapy using insulin glargine and insulin aspart was performed in the early phase, but the treatment was switched to twice-daily biphasic insulin aspart 30 injection because no diabetic complication was present, although the patient was already totally blind and required assistance from his family for self-injection and to improve his quality of life (QOL). Blood glucose control was favorable, and the patient was discharged on April 2.

76 FR 51876 - Medical Devices; Ophthalmic Devices; Classification of the Eyelid Thermal Pulsation System

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-19

... meibomian gland dysfunction (MGD), also known as evaporative dry eye or lipid deficiency dry eye. The system... evaporative dry eye or lipid deficiency dry eye. The system consists of a component that is inserted around...

Dynamic wet-ETEM observation of Pt/C electrode catalysts in a moisturized cathode atmosphere.

PubMed

Yoshida, Kenta; Bright, Alexander N; Ward, Michael R; Lari, Leonardo; Zhang, Xudong; Hiroyama, Tomoki; Boyes, Edward D; Gai, Pratibha L

2014-10-24

The gas injection line of the latest spherical aberration-corrected environmental transmission electron microscope has been modified for achieving real-time/atomic-scale observations in moisturised gas atmospheres for the first time. The newly developed Wet-TEM system is applied to platinum carbon electrode catalysts to investigate the effect of water molecules on the platinum/carbon interface during deactivation processes such as sintering and corrosion. Dynamic in situ movies obtained in dry and 24% moisturised nitrogen environments visualize the rapid rotation, migration and agglomeration of platinum nanoparticles due to the physical adsorption of water and the hydroxylation of the carbon surface. The origin of the long-interconnected aggregation of platinum nanoparticles was discovered to be a major deactivation process in addition to conventional carbon corrosion.

A New Dry Flue Gas Desulfurization Process-Underfeed Circulating Spouted Bed

NASA Astrophysics Data System (ADS)

Tao, M.; Jin, B. S.; Yang, Y. P.

Applying an underfeed system, the underfeed circulating spouted bed was designed as a desulfurization reactor. The main objective of the technology is to improve the mixing effect and distribution uniformity of solid particles, and therefore to advance the desulfurization efficiency and calcium utility. In this article, a series of experimental studies were conducted to investigate the fluidization behavior of the solid-gas two-phase flow in the riser. The results show that the technology can distinctly improve the distribution of gas velocity and particle flux on sections compared with the facefeed style. Analysis of pressure fluctuation signals indicates that the operation parameters have significant influence on the flow field in the reaction bed. The existence of injecting flow near the underfeed nozzle has an evident effect on strengthening the particle mixing.

30 CFR 75.1101-17 - Sealing of dry powder chemical systems.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Sealing of dry powder chemical systems. 75.1101-17 Section 75.1101-17 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...-17 Sealing of dry powder chemical systems. Each dry powder chemical system shall be adequately sealed...

30 CFR 75.1101-17 - Sealing of dry powder chemical systems.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Sealing of dry powder chemical systems. 75.1101-17 Section 75.1101-17 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...-17 Sealing of dry powder chemical systems. Each dry powder chemical system shall be adequately sealed...

30 CFR 75.1101-20 - Safeguards for dry powder chemical systems.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Safeguards for dry powder chemical systems. 75...-20 Safeguards for dry powder chemical systems. Adequate guards shall be provided along all belt conveyors in the vicinity of each dry powder chemical system to protect persons whose vision is restricted...

30 CFR 75.1101-20 - Safeguards for dry powder chemical systems.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Safeguards for dry powder chemical systems. 75...-20 Safeguards for dry powder chemical systems. Adequate guards shall be provided along all belt conveyors in the vicinity of each dry powder chemical system to protect persons whose vision is restricted...

30 CFR 75.1101-17 - Sealing of dry powder chemical systems.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Sealing of dry powder chemical systems. 75.1101-17 Section 75.1101-17 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...-17 Sealing of dry powder chemical systems. Each dry powder chemical system shall be adequately sealed...

30 CFR 75.1101-20 - Safeguards for dry powder chemical systems.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Safeguards for dry powder chemical systems. 75...-20 Safeguards for dry powder chemical systems. Adequate guards shall be provided along all belt conveyors in the vicinity of each dry powder chemical system to protect persons whose vision is restricted...

30 CFR 75.1101-20 - Safeguards for dry powder chemical systems.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Safeguards for dry powder chemical systems. 75...-20 Safeguards for dry powder chemical systems. Adequate guards shall be provided along all belt conveyors in the vicinity of each dry powder chemical system to protect persons whose vision is restricted...

30 CFR 75.1101-17 - Sealing of dry powder chemical systems.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Sealing of dry powder chemical systems. 75.1101-17 Section 75.1101-17 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...-17 Sealing of dry powder chemical systems. Each dry powder chemical system shall be adequately sealed...

Mechanisms of deterioration of nutrients. [of freeze dried foods

NASA Technical Reports Server (NTRS)

Karel, M.; Flink, J. M.

1976-01-01

Methods which produce freeze dried foods of improved quality were examined with emphasis on storage stability. Specific topics discussed include: microstructure of freeze dried systems, investigation of structural changes in freeze dried systems, artificial food matrices, osmotic preconcentration to yield improved quality freeze dried fruits, and storage stability of osmotically preconcentrated freeze dried fruits.

Effects of dietary energy allowance and decline in dry matter intake during the dry period on responses to glucose and insulin in transition dairy cows.

PubMed

Salin, S; Vanhatalo, A; Elo, K; Taponen, J; Boston, R C; Kokkonen, T

2017-07-01

We assessed whether high energy intake during the early dry period [144% of metabolizable energy (ME) requirements/d] followed by a gradual restriction of energy intake in the close-up dry period (119% of ME/d; HEI) impaired whole-body insulin sensitivity compared with a controlled energy intake (100% of ME/d; CEI) throughout the 6-wk dry period. Multiparous Ayrshire dairy cows (n = 16) were blocked by body weight, body condition score, and expected date of parturition and were used in a randomized complete block design until 10 d after parturition. Cows were fed either HEI or CEI diets based on grass silage during the first 3 wk of the dry period and grass silage supplemented with a commercial concentrate (30% of ME intake) during the final 3 wk of gestation. After calving, all cows were fed grass silage ad libitum and an increasing amount of commercial concentrate (maximum 9 kg at d 10 postpartum). Intravenous glucose tolerance tests (IVGTT) and intravenous insulin challenges were performed -10 ± 5 d (n = 15) and +10 ± 1 d (n = 14) relative to parturition. Following glucose injection, we did not find any treatment effects on glucose and insulin responses. The prepartal nonesterified fatty acid (NEFA) response of the HEI group was blunted, basal NEFA and the decrement of NEFA were smaller, and the area under the response curve (AUC) of NEFA was less negative in HEI cows than in CEI cows. The NEFA response reversed after parturition; the NEFA AUC of the HEI group was more negative than that of the CEI group. We did not find similar responses after insulin injection. Across the treatments, NEFA AUC correlated strongly with the basal NEFA concentration during the IVGTT pre- and postpartum. Calculated and model-based indices characterizing the overall glucose tolerance and β-cell function and the insulin sensitivity were higher after parturition than during the dry period. Consistent with the lower basal insulin, the acute insulin release after the glucose infusion was smaller in postpartal IVGTT than in prepartal IVGTT. The results suggest that whole-body insulin sensitivity of the cows increased after parturition. However, the role of peripheral insulin sensitivity in the regulation of glucose partitioning seems to be minor relative to the major change in insulin secretion and clearance during the periparturient period. Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Modifying the release of leuprolide from spray dried OED microparticles.

PubMed

Alcock, R; Blair, J A; O'Mahony, D J; Raoof, A; Quirk, A V

2002-08-21

A range of oligosaccharide ester derivatives (OEDs) have been designed as drug delivery matrices for controlled release. The synthetic hormone analogue, leuprolide, was encapsulated within these matrices using hydrophobic ion pairing and solvent spray drying. The particles produced modified the release of leuprolide in vitro (dissolution in phosphate buffered saline) and in vivo (subcutaneous and pulmonary delivery in the rat). Release rate was dependent on drug loading and could be manipulated by choice of OED and by combining different OEDs in different ratios. Leuprolide encapsulated in the OEDs retained biological activity as evidenced by elevation in plasma luteinising hormone levels following subcutaneous injection of leuprolide recovered from OED particles in vitro prior to in vivo administration.

An investigation of the performance of an electronic in-line pump system for diesel engines

NASA Astrophysics Data System (ADS)

Fan, Li-Yun; Zhu, Yuan-Xian; Long, Wu-Qiang; Ma, Xiu-Zhen; Xue, Ying-Ying

2008-12-01

WIT Electronic Fuel System Co., Ltd. has developed a new fuel injector, the Electronic In-line Pump (EIP) system, designed to meet China’s diesel engine emission and fuel economy regulations. It can be used on marine diesel engines and commercial vehicle engines through different EIP systems. A numerical model of the EIP system was built in the AMESim environment for the purpose of creating a design tool for engine application and system optimization. The model was used to predict key injection characteristics under different operating conditions, such as injection pressure, injection rate, and injection duration. To validate these predictions, experimental tests were conducted under the conditions that were modeled. The results were quite encouraging and in agreement with model predictions. Additional experiments were conducted to study the injection characteristics of the EIP system. These results show that injection pressure and injection quantity are insensitive to injection timing variations, this is due to the design of the constant velocity cam profile. Finally, injection quantity and pressure vs. pulse width at different cam speeds are presented, an important injection characteristic for EIP system calibration.

Lack of a synergistic effect of arginine-glutamic acid on the physical stability of spray-dried bovine serum albumin.

PubMed

Reslan, Mouhamad; Demir, Yusuf K; Trout, Bernhardt L; Chan, Hak-Kim; Kayser, Veysel

2017-09-01

Improving the physical stability of spray-dried proteins is essential for enabling pulmonary delivery of biotherapeutics as a noninvasive alternative to injections. Recently, a novel combination of two amino acids - l-arginine (l-Arg) and l-glutamic acid (l-Glu), has been reported to have synergistic protein-stabilizing effects on various protein solutions. Using spray-dried bovine serum albumin (BSA) reconstituted in solution as a model protein, we investigated the synergistic effect of these amino acids on the physical stability of proteins. Five BSA solutions were prepared: (1) BSA with no amino acids (control); (2) with 50 mM l-Arg; (3) with 200 mM l-Arg, (4) with 50 mM l-Glu and (5) with 25:25 mM of Arg:Glu. All solutions were spray-dried and accelerated studies at high temperatures were performed. Following accelerated studies, monomer BSA loss was measured using SE-HPLC. We found that l-Arg significantly improved the physical stability of spray-dried BSA even at low concentrations, however, when combined with l-Glu, was ineffective at reducing monomer BSA loss. Our findings demonstrate the limitations in using Arg-Glu for the stabilization of spray-dried BSA. Furthermore, we found that a low concentration of l-Glu enhanced monomer BSA loss. These findings may have significant implications on the design of future biotherapeutic formulations.

Effect of chitosan-N-acetylcysteine conjugate in a mouse model of botulinum toxin B-induced dry eye.

PubMed

Hongyok, Teeravee; Chae, Jemin J; Shin, Young Joo; Na, Daero; Li, Li; Chuck, Roy S

2009-04-01

To evaluate the effect of a thiolated polymer lubricant, chitosan-N-acetylcysteine conjugate (C-NAC), in a mouse model of dry eye. Eye drops containing 0.5% C-NAC, 0.3% C-NAC, a vehicle (control group), artificial tears, or fluorometholone were applied in a masked fashion in a mouse model of induced dry eye from 3 days to 4 weeks after botulinum toxin B injection. Corneal fluorescein staining was periodically recorded. Real-time reverse transcriptase-polymerase chain reaction and immunofluorescence staining were performed at the end of the study to evaluate inflammatory cytokine expressions. Mice treated with C-NAC, 0.5%, and fluorometholone showed a downward trend that was not statistically significant in corneal staining compared with the other groups. Chitosan-NAC formulations, fluorometholone, and artificial tears significantly decreased IL-1beta (interleukin 1beta), IL-10, IL-12alpha, and tumor necrosis factor alpha expression in ocular surface tissues. The botulinum toxin B-induced dry eye mouse model is potentially useful in evaluating new dry eye treatment. Evaluation of important molecular biomarkers suggests that C-NAC may impart some protective ocular surface properties. However, clinical data did not indicate statistically significant improvement of tear production and corneal staining in any of the groups tested. Topically applied C-NAC might protect the ocular surface in dry eye syndrome, as evidenced by decreased inflammatory cytokine expression.

[Effect of thalidomide in a mouse model of paraquat-induced acute lung injury and the underlying mechanisms].

PubMed

Li, Dong; Xu, Li-yan; Chang, Zi-juan; Zhao, Guang-ju; Nan, Chao; Lu, Zhong-qiu

2013-03-01

To investigate the intervention effect of thalidomide on paraquat-induced acute lung injury in mice and its mechanism. Male ICR mice were randomly allocated to negative control group (n = 30), thalidomide control group (n = 30), paraquat poisoning group (n = 30), 50 mg/kg thalidomide treatment group (n = 30), 100 mg/kg thalidomide treatment group (n = 30), and 150 mg/kg thalidomide treatment group (n = 30). The negative control group was intraperitoneally injected with the same volume of saline; the thalidomide control group was intraperitoneally injected with thalidomide (150 mg/kg); the paraquat poisoning group was intraperitoneally injected with diluted paraquat solution (22 mg/kg); each thalidomide treatment group was intraperitoneally injected with the same volume of paraquat solution (22 mg/kg) and was injected with thalidomide (50, 100, or 150 mg/kg) 1 h later. All mice were anesthetized and sacrificed at 1, 3, or 7 d after paraquat poisoning, and their lung tissue was collected. The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in lung tissue were measured by double-antibody sandwich ELISA; the mRNA expression of nuclear factor-kappa B (NF-κB) was measured by RT-PCR; the protein expression of nuclear NF-kgr;B p65 was measured by Western blot. The pathological changes of lung tissue were observed under light microscope; the wet/dry ratio of the lung was calculated. Compared with the negative control group, the paraquat poisoning group had significantly increased levels of TNF-α, IL-1β, IL-6, NF-κB mRNA, and nuclear NF-κB p65 and wet/dry ratio of the lung (P < 0.05). Compared with the paraquat poisoning group, the thalidomide treatment groups had significantly decreased levels of TNF-α, IL-1β, IL-6, NF-κB mRNA, and nuclear NF-κB p65 and wet/dry ratios of the lung (P < 0.05), and the 150 mg/kg thalidomide treatment group showed the most significant decrease in the levels of TNF-α, IL-1β, IL-6, NF-κB mRNA, and nuclear NF-κB p65. The observation of pathological changes showed that the paraquat poisoning group had the most marked lung tissue damage at 3 d after poisoning, and the lung tissue damage was lessened in the thalidomide treatment groups. Thalidomide can reduce paraquat-induced acute lung injury and lung edema. The mechanism may include inhibition of NF-κB activation and expression and downregulation of TNF-α, IL-1β, and IL-6.

THE CHARACTERIZATION OF A SOLID SORBENT WITH CRYSTALLITE SIZE AND STRAIN DATA FROM X-RAY DIFFRACTION LINE BROADENING

EPA Science Inventory

The paper gives results of the characterization of a solid sorbent with crystallite size and strain data from x-ray diffraction line broadening, as part of an EPA investigation of the injection of dry Ca(OH)2 into coal-fired electric power plant burners for the control of SO2 emi...

Tracheobronchial Cast Production and Use in an Undergraduate Human Anatomy Course

ERIC Educational Resources Information Center

Cope, Lee Anne

2008-01-01

Silastic E RTV silicone was used to produce tracheobronchial cast for use in an undergraduate human anatomy course. Following air-drying, the trachea and lungs were injected with E RTV silicone and allowed to cure for 24 hr. The parenchyma was then removed from the tracheobronchial cast by maceration and boiling and then whitened in a 10% solution…

High-resolution synchrotron X-ray analysis of bioglass-enriched hydrogels.

PubMed

Gorodzha, Svetlana; Douglas, Timothy E L; Samal, Sangram K; Detsch, Rainer; Cholewa-Kowalska, Katarzyna; Braeckmans, Kevin; Boccaccini, Aldo R; Skirtach, Andre G; Weinhardt, Venera; Baumbach, Tilo; Surmeneva, Maria A; Surmenev, Roman A

2016-05-01

Enrichment of hydrogels with inorganic particles improves their suitability for bone regeneration by enhancing their mechanical properties, mineralizability, and bioactivity as well as adhesion, proliferation, and differentiation of bone-forming cells, while maintaining injectability. Low aggregation and homogeneous distribution maximize particle surface area, promoting mineralization, cell-particle interactions, and homogenous tissue regeneration. Hence, determination of the size and distribution of particles/particle agglomerates in the hydrogel is desirable. Commonly used techniques have drawbacks. High-resolution techniques (e.g., SEM) require drying. Distribution in the dry state is not representative of the wet state. Techniques in the wet state (histology, µCT) are of lower resolution. Here, self-gelling, injectable composites of Gellan Gum (GG) hydrogel and two different types of sol-gel-derived bioactive glass (bioglass) particles were analyzed in the wet state using Synchrotron X-ray radiation, enabling high-resolution determination of particle size and spatial distribution. The lower detection limit volume was 9 × 10(-5) mm(3) . Bioglass particle suspensions were also studied using zeta potential measurements and Coulter analysis. Aggregation of bioglass particles in the GG hydrogels occurred and aggregate distribution was inhomogeneous. Bioglass promoted attachment of rat mesenchymal stem cells (rMSC) and mineralization. © 2016 Wiley Periodicals, Inc.

Mechanisms of deterioration of nutrients. [improved quality of freeze-dried foods

NASA Technical Reports Server (NTRS)

Karel, M.; Flink, J. M.

1978-01-01

Methods for improving the quality of freeze-dried foods were investigated. Areas discussed include: (1) microstructure of freeze-dried systems, (2) structural changes in freeze-dried systems, (3) artificial food matrices, and (4) osmotic preconcentration to yield improved freeze-dried products.

In-injection port thermal desorption for explosives trace evidence analysis.

PubMed

Sigman, M E; Ma, C Y

1999-10-01

A gas chromatographic method utilizing thermal desorption of a dry surface wipe for the analysis of explosives trace chemical evidence has been developed and validated using electron capture and negative ion chemical ionization mass spectrometric detection. Thermal desorption was performed within a split/splitless injection port with minimal instrument modification. Surface-abraded Teflon tubing provided the solid support for sample collection and desorption. Performance was characterized by desorption efficiency, reproducibility, linearity of the calibration, and method detection and quantitation limits. Method validation was performed with a series of dinitrotoluenes, trinitrotoluene, two nitroester explosives, and one nitramine explosive. The method was applied to the sampling of a single piece of debris from an explosion containing trinitrotoluene.

The effectiveness of a mail reminder system for depot medroxyprogesterone injections.

PubMed

Madlon-Kay, D J

1996-04-01

To determine if a mail reminder system leads to an increase in the percentage of depot medroxyprogesterone acetate (Depo-Provera) injections administered on time, the charts of 184 women were reviewed. The reminder postcard included the due dates of both the next medroxyprogesterone injection and the next Papanicolaou test. Timeliness of injections improved with the reminder system from 64% administered on time to 76% (P < .02). Injections given late despite the reminders were late a mean of 8 days. Injections given late before the reminder system began were late a mean of 20 days (P < .05). If injections given during the injection's 14-day "grace" period are included, the on-time rate improved from 87% to 96% with the reminder system (P < .005). The reminder system was not effective in ensuring annual Papanicolaou testing. Vigilance is needed to ensure that women receiving medroxyprogesterone injections also receive timely Papanicolaou testing.

A volumetric flow sensor for automotive injection systems

NASA Astrophysics Data System (ADS)

Schmid, U.; Krötz, G.; Schmitt-Landsiedel, D.

2008-04-01

For further optimization of the automotive power train of diesel engines, advanced combustion processes require a highly flexible injection system, provided e.g. by the common rail (CR) injection technique. In the past, the feasibility to implement injection nozzle volumetric flow sensors based on the thermo-resistive measurement principle has been demonstrated up to injection pressures of 135 MPa (1350 bar). To evaluate the transient behaviour of the system-integrated flow sensors as well as an injection amount indicator used as a reference method, hydraulic simulations on the system level are performed for a CR injection system. Experimentally determined injection timings were found to be in good agreement with calculated values, especially for the novel sensing element which is directly implemented into the hydraulic system. For the first time pressure oscillations occurring after termination of the injection pulse, predicted theoretically, could be verified directly in the nozzle. In addition, the injected amount of fuel is monitored with the highest resolution ever reported in the literature.

Mathematical and computational modeling simulation of solar drying Systems

USDA-ARS?s Scientific Manuscript database

Mathematical modeling of solar drying systems has the primary aim of predicting the required drying time for a given commodity, dryer type, and environment. Both fundamental (Fickian diffusion) and semi-empirical drying models have been applied to the solar drying of a variety of agricultural commo...

Compton Dry-Cask Imaging System

ScienceCinema

None

2017-12-09

The Compton-Dry Cask Imaging Scanner is a system that verifies and documents the presence of spent nuclear fuel rods in dry-cask storage and determines their isotopic composition without moving or opening the cask. For more information about this project, visit http://www.inl.gov/rd100/2011/compton-dry-cask-imaging-system/

Rates of fuel discharge as affected by the design of fuel-injection systems for internal-combustion engines

NASA Technical Reports Server (NTRS)

Gelalles, A G; Marsh, E T

1933-01-01

Using the method of weighing fuel collected in a receiver during a definite interval of the injection period, rates of discharge were determined, and the effects noted, when various changes were made in a fuel-injection system. The injection system consisted primarily of a by-pass controlled fuel pump and an automatic injection valve. The variables of the system studied were the pump speed, pump-throttle setting, discharge-orifice diameter, injection-valve opening and closing pressures, and injection-tube length and diameter.

Space and Industrial Brine Drying Technologies

NASA Technical Reports Server (NTRS)

Jones, Harry W.; Wisniewski, Richard S.; Flynn, Michael; Shaw, Hali

2014-01-01

This survey describes brine drying technologies that have been developed for use in space and industry. NASA has long considered developing a brine drying system for the International Space Station (ISS). Possible processes include conduction drying in many forms, spray drying, distillation, freezing and freeze drying, membrane filtration, and electrical processes. Commercial processes use similar technologies. Some proposed space systems combine several approaches. The current most promising candidates for use on the ISS use either conduction drying with membrane filtration or spray drying.

Comparative Research on Characteristics of the Isolation Systems with Dry Friction Damping and with Vicious Damping under Base Excitation

NASA Astrophysics Data System (ADS)

Hou, Junfang; jing, Min; Zhang, Weihua; Lu, Yahui; He, Haiwen

2017-12-01

As for the isolation problem of electronic equipments on vehicle, the vibration response characteristics of dry friction damping isolation system under base displacement excitation was analyzed in theory by harmonic balance method, and the displacement response was compared between the isolation systems with dry friction damping and vicious damping separately. The results show that the isolation system with small dry friction damping can’t meet the demands of displacement reduction close to the natural frequency, and it can realize full-frequency vibration isolation by improving dry friction damping when the lock frequency passes beyond the resonance frequency band. The results imply that the damping mechanism of dry friction isolator can’t be described only by dry friction damping, and the composite damping with dry friction and vicious damping is more appropriate.

Uterine, ovarian, and production responses of lactating dairy cows to increasing dietary concentrations of menhaden fish meal.

PubMed

Mattos, R; Staples, C R; Williams, J; Amorocho, A; McGuire, M A; Thatcher, W W

2002-04-01

The primary objective was to determine whether the dietary polyunsaturated fatty acids, eicosapentaenoic (EPA, C20:5, n-3) and docosahexaenoic (DHA, C22:6, n-3), present in fish meal (FM) can attenuate uterine secretion of PGF2alpha in response to a challenge with estradiol and oxytocin in lactating dairy cows. Cycling multiparous cows (n = 32) were fed diets containing 0 (OFM), 2.6 (2.6FM), 5.2 (5.2FM), or 7.8% menhaden FM (7.8FM). The diet consisting of 7.8FM also contained fish oil (0.28% of dietary dry matter) to increase intake of EPA and DHA. Average dry matter intake was 24.9 kg/d and unaffected by diet. Combined intakes of EPA and DHA averaged 0, 12.8, 24.1, and 54.0 g/d from the OFM, 2.6FM, 5.2FM, and 7.8FM diets, respectively. At 30 to 34 d after initiation of dietary treatments, cows received an i.m. injection of 100 microg of GnRH followed by i.m. administration of 25 and 15 mg of PGF2alpha after 7 and 8 d, respectively. Synchronous ovulation was induced by an injection of 3000 IU of human chorionic gonadotropin (hCG) given 24 h later on d 9. Subsequent luteal phase increases in plasma progesterone concentrations did not differ (0.88 ng/ml per day). At 15 d after hCG injection, cows were injected with estradiol-17beta (3 mg, i.v.) at 0900 h and oxytocin (100 IU, i.v.) at 1300 h. Plasma PGF2alpha metabolite concentrations after oxytocin injection were reduced in cows fed diets containing FM compared with those fed OFM. Milk production (39.1 kg/d) and concentrations of fat, protein, or urea nitrogen in milk were not affected by diet. Feeding fish meal and fish oil containing eicosapentaenoic acid and docosahexaenoic acid reduced the proportion of n-6 fatty acids and increased that of n-3 fatty acids in milk in a dose-responsive manner.

Effect of Drying on Heavy Metal Fraction Distribution in Rice Paddy Soil

PubMed Central

Qi, Yanbing; Huang, Biao; Darilek, Jeremy Landon

2014-01-01

An understanding of how redox conditions affect soil heavy metal fractions in rice paddies is important due to its implications for heavy metal mobility and plant uptake. Rice paddy soil samples routinely undergo oxidation prior to heavy metal analysis. Fraction distribution of Cu, Pb, Ni, and Cd from paddy soil with a wide pH range was investigated. Samples were both dried according to standard protocols and also preserved under anaerobic conditions through the sampling and analysis process and heavy metals were then sequentially extracted for the exchangeable and carbonate bound fraction (acid soluble fraction), iron and manganese oxide bound fraction (reducible fraction), organic bound fraction (oxidizable fraction), and residual fraction. Fractions were affected by redox conditions across all pH ranges. Drying decreased reducible fraction of all heavy metals. Curesidual fraction, Pboxidizable fraction, Cdresidual fraction, and Niresidual fraction increased by 25%, 33%, 35%, and >60%, respectively. Pbresidual fraction, Niacid soluble fraction, and Cdoxidizable fraction decreased 33%, 25%, and 15%, respectively. Drying paddy soil prior to heavy metal analysis overestimated Pb and underestimated Cu, Ni, and Cd. In future studies, samples should be stored after injecting N2 gas to maintain the redox potential of soil prior to heavy metal analysis, and investigate the correlation between heavy metal fraction distribution under field conditions and air-dried samples. PMID:24823670

Influence of Water Content on Mechanical Properties of Rock in Both Saturation and Drying Processes

NASA Astrophysics Data System (ADS)

Zhou, Zilong; Cai, Xin; Cao, Wenzhuo; Li, Xibing; Xiong, Cheng

2016-08-01

Water content has a pronounced influence on the properties of rock materials, which is responsible for many rock engineering hazards, such as landslides and karst collapse. Meanwhile, water injection is also used for the prevention of some engineering disasters like rock-bursts. To comprehensively investigate the effect of water content on mechanical properties of rocks, laboratory tests were carried out on sandstone specimens with different water contents in both saturation and drying processes. The Nuclear Magnetic Resonance technique was applied to study the water distribution in specimens with variation of water contents. The servo-controlled rock mechanics testing machine and Split Hopkinson Pressure Bar technique were used to conduct both compressive and tensile tests on sandstone specimens with different water contents. From the laboratory tests, reductions of the compressive and tensile strength of sandstone under static and dynamic states in different saturation processes were observed. In the drying process, all of the saturated specimens could basically regain their mechanical properties and recover its strength as in the dry state. However, for partially saturated specimens in the saturation and drying processes, the tensile strength of specimens with the same water content was different, which could be related to different water distributions in specimens.

Successful lyophilization by adopting a fast ramp rate during primary drying in protein formulations.

PubMed

Ohori, Ryo; Akita, Tomomi; Yamashita, Chikamasa

2018-06-15

In the lyophilization process for injections, the shelf temperature (T s ) and chamber pressure (P c ) have mainly been investigated to optimize the primary drying process. The objective of this study was to show that lyophilization of protein formulations can be achieved by adopting a fast ramp rate of T s in the beginning of the primary drying process. Bovine serum albumin was used as the model protein, and seven different lyophilized formulations obtained were stored at elevated temperature. We found that although acceptable cake appearance was confirmed by the fast ramp cycle, all formulations of lyophilized cakes obtained by the slow ramp cycle severely collapsed (macrocollapse). It is thought that the collapse in the slow ramp cycle occurred during the shelf ramp in the beginning of primary drying and that insufficient removal of water from the dried matrix caused viscous flow (product collapse). Regarding storage stability, moisture-induced degradation was confirmed in some of the formulations prepared by the slow ramp cycle, whereas all lyophilized BSA formulations prepared by the fast ramp cycle were stable. Thus, the results indicate that the ramp rate appears to be one of the critical operational parameters required to establish a successful lyophilization cycle. Copyright © 2018. Published by Elsevier B.V.

Deposition pattern and tracer particle motion of evaporating multi-component sessile droplets.

PubMed

Amjad, Muhammad; Yang, Yang; Raza, Ghulam; Gao, Hui; Zhang, Jun; Zhou, Leping; Du, Xiaoze; Wen, Dongsheng

2017-11-15

The understanding of near-wall motion, evaporation behavior and dry pattern of sessile nanofluid droplets is fundamental to a wide range of applications such as painting, spray drying, thin film coating, fuel injection and inkjet printing. However, a deep insight into the heat transfer, fluid flow, near-wall particle velocity and their effects on the resulting dry patterns is still much needed to take the full advantage of these nano-sized particles in the droplet. This work investigates the effect of direct absorptive silicon/silver (Si/Ag) hybrid nanofluids via two experiments. The first experiment identifies the motion of tracer particles near the triple line of a sessile nanofluid droplet on a super-hydrophilic substrate under ambient conditions by the multilayer nanoparticle image velocimetry (MnPIV) technique. The second experiment reveals the effect of light-sensitive Si/Ag composite nanoparticles on the droplet evaporation rate and subsequent drying patterns under different radiation intensities. The results show that the presence of nanoparticle in a very small proportion significantly affects the motion of tracer particles, leading to different drying patterns and evaporation rates, which can be very important for the applications such as spray coating and inkjet printing. Copyright © 2017 Elsevier Inc. All rights reserved.

Skin vaccination with live virus vectored microneedle arrays induce long lived CD8(+) T cell memory.

PubMed

Becker, Pablo D; Hervouet, Catherine; Mason, Gavin M; Kwon, Sung-Yun; Klavinskis, Linda S

2015-09-08

A simple dissolvable microneedle array (MA) platform has emerged as a promising technology for vaccine delivery, due to needle-free injection with a formulation that preserves the immunogenicity of live viral vectored vaccines dried in the MA matrix. While recent studies have focused largely on design parameters optimized to induce primary CD8(+) T cell responses, the hallmark of a vaccine is synonymous with engendering long-lasting memory. Here, we address the capacity of dried MA vaccination to programme phenotypic markers indicative of effector/memory CD8(+) T cell subsets and also responsiveness to recall antigen benchmarked against conventional intradermal (ID) injection. We show that despite a slightly lower frequency of dividing T cell receptor transgenic CD8(+) T cells in secondary lymphoid tissue at an early time point, the absolute number of CD8(+) T cells expressing an effector memory (CD62L(-)CD127(+)) and central memory (CD62L(+)CD127(+)) phenotype during peak expansion were comparable after MA and ID vaccination with a recombinant human adenovirus type 5 vector (AdHu5) encoding HIV-1 gag. Similarly, both vaccination routes generated CD8(+) memory T cell subsets detected in draining LNs for at least two years post-vaccination capable of responding to secondary antigen. These data suggest that CD8(+) T cell effector/memory generation and long-term memory is largely unaffected by physical differences in vaccine delivery to the skin via dried MA or ID suspension. Copyright © 2015 Elsevier Ltd. All rights reserved.

Reconnaissance Assessment of the Potential for Roadside Dry Wells to Affect Water Quality on the Island of Hawai'i

USGS Publications Warehouse

Izuka, Scot K.; Senter, Craig A.; Johnson, Adam G.

2009-01-01

The County of Hawai'i Department of Public Works (DPW) uses dry wells to dispose of stormwater runoff from roads. Recently, concern has been raised that water entering the dry wells may transport contaminants to groundwater and affect the quality of receiving waters. The DPW operates 2,052 dry wells. Compiling an inventory of these dry wells and sorting it on the basis of presence or absence of urbanization in the drainage area, distance between the bottom of the dry well and the water table, and proximity to receiving waters helps identify the dry wells having greatest potential to affect the quality of receiving waters so that future studies or mitigation efforts can focus on a smaller number of dry wells. The drainage areas of some DPW dry wells encompass urbanized areas, which could be a source of contaminants. Some dry wells penetrate close to or through the water table, eliminating or substantially reducing opportunities for contaminant attenuation between the ground surface and water table. Dry wells that have drainage areas that encompass urbanization, penetrate to near the water table, and are near the coast have the highest potential to affect the quality of coastal waters (this study did not consider specific sections of coastline that may be of greater concern than others). Some DPW dry wells, including a few that have drainage areas that encompass urbanization, lie within the areas contributing recharge (ACR) to drinking-water wells. Numerical groundwater modeling studies by previous investigators indicate that water infiltrating those dry wells could eventually be pumped at drinking-water wells. Dry wells that have a high potential for affecting coastal receiving waters or drinking-water wells can be the focus of studies to further understand the effect of the dry wells on the quality of receiving waters. Possible study approaches include sampling for contaminants at the dry well and receiving water, injecting and monitoring the movement of tracers, and numerical modeling. To fully assess whether dry wells actually pose a significant contamination threat to receiving waters, results from modeling or monitoring must be compared to limits for contaminant concentration at receiving waters. These limits are usually established by the agencies tasked with protecting those waters.

Tritium behavior pattern in some soil-plant systems in a tropical environment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Soma, S.D.; Iyengar, T.S.; Sadarangani, S.H.

1975-01-01

A study of the distribution pattern of tritium in the soil/plant environment gives a valuable ecological information on the natural water balance. The results of such a study for the conditions obtaining in India are given in this paper. Field studies are carried out by injection of tritium into some soil/ plant systems and following the transfer pathways. The method of extraction for tissue-free-water-tritium (TFWT) is based on the vacuum freeze-drying technique while the tissue-bound-tritium (TBT) is estimated by a modified version of the Shoniger method. The determination of residence time of tritium in aqueous and organic phase in amore » number of tropical trees has been carried out both for stem- injection as well as intake from the soil. From the results of this study the tree biomass and transpiration rates have been determined. The tritium profile over time, for an acute exposure in certain trees such as Morinda Tinetoria, Achras Sapota etc. shows significantly different patterns compared to the normal pattern shown by Mangifera Indica, Terminalia Catappa, Ficus Glomerata etc. The period of investigation in each case varied from 400 to 1000 h. In most of the cases, the TBT fractions were very low compared to TFWT fractions in the initial stages. The tritium behaviour in the tree reflects significant characteristics of the tritium behaviour in the soil system. The authors have found that the leaf sampling can be used as an indicator of total environmental tritium behaviour. (auth)« less

Vascular labeling of the head and neck vessels: Technique, advantages and limitations.

PubMed

Gálvez, Alba; Caraballo, José-Leonardo; Manzanares-Céspedes, María-Cristina; Valdivia-Gandur, Iván; Figueiredo, Rui; Valmaseda-Castellón, Eduard

2017-05-01

Vascular staining techniques have been used to describe the vascular structures of several anatomic areas. However, few reports have described this procedure in the head and neck region. This paper describes a head and neck vascular labeling procedure, and describes some of the technical complications that may occur. Fifteen specimen cadaver heads were prepared. After drying the vascular system, the internal carotid arteries were ligated and a solution with latex and a gelling agent was injected into the internal carotid arteries and external jugular veins. Two different colors were employed to differentiate arteries from veins. A total of 60ml latex was injected into each blood vessel. Subsequently, the specimens were refrigerated at 5°C for a minimum of 24 hours. Finally, a dissection was performed to identify the venous and arterial systems of the maxillofacial region. In most specimens, correct identification of the vascular structures (lingual artery, pterigoyd plexus, and the major palatal arteries, among others) was possible. However, in three heads a major technical problem occurred (the latex remained liquid), making the dissection unfeasible. Other minor complications such as latex obstruction due to the presence of atheromas were found in two further specimens. The vascular labeling technique is a predictable, effective and simple method for analyzing the vascular system of the maxillofacial area in cadaveric studies, including vessels of reduced diameter or with an intraosseous course. This procedure can be especially useful to teach vascular anatomy to dental students and postgraduate residents. Key words: Blood vessels, vascular casting, vascular labeling, head and neck arteries, carotid arteries, jugular veins.

Temperature Control System for Mushroom Dryer

NASA Astrophysics Data System (ADS)

Wibowo, I. A.; Indah, Nur; Sebayang, D.; Adam, N. H.

2018-03-01

The main problem in mushroom cultivation is the handling after the harvest. Drying is one technique to preserve the mushrooms. Traditionally, mushrooms are dried by sunshine which depends on the weather. This affects the quality of the dried mushrooms. Therefore, this paper proposes a system to provide an artificial drying for mushrooms in order to maintain their quality. The objective of the system is to control the mushroom drying process to be faster compared to the natural drying at an accurate and right temperature. A model of the mushroom dryer has been designed, built, and tested. The system comprises a chamber, heater, blower, temperature sensor and electronic control circuit. A microcontroller is used as the controller which is programmed to implement a bang-bang control that regulates the temperature of the chamber. A desired temperature is inputted as a set point of the control system. Temperature of 45 °C is chosen as the operational drying temperature. Several tests have been carried out to examine the performance of the system including drying speed, the effects of ambient conditions, and the effects of mushroom size. The results show that the system can satisfy the objective.

A Science-Based Understanding of Cermet Processing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cesarano, III, Joseph; Roach, Robert Allen; Kilgo, Alice C.

2006-04-01

This report is a summary of the work completed in FY01 for science-based characterization of the processes used to fabricate 1) cermet vias in source feedthrus using slurry and paste-filling techniques and 2) cermet powder for dry pressing. Common defects found in cermet vias were characterized based on the ability of subsequent processing techniques (isopressing and firing) to remove the defects. Non-aqueous spray drying and mist granulation techniques were explored as alternative methods of creating CND50, the powder commonly used for dry pressed parts. Compaction and flow characteristics of these techniques were analyzed and compared to standard dry-ball-milled CND50. Duemore » to processing changes, changes in microstructure can occur. A microstructure characterization technique was developed to numerically describe cermet microstructure. Machining and electrical properties of dry pressed parts were also analyzed and related to microstructure using this analytical technique.3 Executive SummaryThis report outlines accomplishments in the science-based understanding of cermet processing up to fiscal year 2002 for Sandia National Laboratories. The three main areas of work are centered on 1) increasing production yields of slurry-filled cermets, 2) evaluating the viability of high-solids-loading pastes for the same cermet components, and 3) optimizing cermet powder used in pressing processes (CND50). An additional development that was created as a result of the effort to fully understand the impacts of alternative processing techniques is the use of analytical methods to relate microstructure to physical properties. Recommendations are suggested at the end of this report. Summaries of these four efforts are as follows:1.Increase Production Yields of Slurry-Filled Cermet Vias Finalized slurry filling criteria were determined based on three designs of experiments where the following factors were analyzed: vacuum time, solids loading, pressure drop across the filter paper, slurry injection rate, via prewetting, slurry injection angle, filter paper prewetting, and slurry mixing time. Many of these factors did not have an influence on defect formation. In order of decreasing importance, critical factors for defect formation by slurry filling are vacuum time (20 sec. optimal), slurry solids loading (20.0 g of cermet with 13.00 g of DGBEA solvent (21.2 vol%)), filling with the pipette in a vertical position, and faster injection rates (%7E765 l/s) as preferable to slower. No further recommendations for improvement to this process can be suggested. All findings of the slurry filling process have been transferred to CeramTec, the supplier. Paste filling methods appear to show more promise of increasing production yields. The types of flaws commonly found in slurry-filled vias were identified and followed throughout the entire source feedthru process. In general, all sizes of cracks healed during isopressing and firing steps. Additionally, small to medium sized voids (less than 1/3 the via diameter) can be healed. Porosity will usually lead to via necking, which may cause the part to be out of specification. Large voids (greater 4 than 1/3 of the diameter) and partial fills are not healed or produce significant necking. 2.Viability of High-Solids-Loading-Cermet Paste for Filling Source Feedthru ViaThe paste-filling process is easy to implement and easier to use. The high solids loading (>40 vol %) reduces the incidence of drying defects, which are seen in slurry filled (%7E23 vol %) vias. Additionally, the way in which the vias are filled (the paste is pushed from entrance to exit, displacing air as the paste front progresses), reduces the chance of entrapped voids, which are common in the slurry filling process. From the fair number of samples already filled, the likelihood of this process being a viable and reliable process is very good. Issues of concern for the paste process, as with any new process, are any problems that may arise in subsequent manufacturing stages of the neutron tube that may be affected by subtle changes in microstructure. Both MC4277 and MC4300-type source feedthrus were paste-filled by hand. X-ray analysis showed a much lower existence of voids in the green parts as compared to slurry-filled parts. The paste shows improvements in shelf life (weeks) as compared to slurry (minutes). This method of introducing the cermet to the via also lends itself very well to an automated filling process where a machine can either drill vias or, with the aid of a vision system, find pre-drilled vias and fill them with paste. The pastes used in this work prove the concept of this automated filling process as MC4277 sources have been filled using such a prototype machine, however, better performing pastes can be developed which are less hazardous (aqueous systems). The paste process was also used to successfully fill MC4300 "dogleg" type sources.3.Optimize CND50 Two methods of creating granulated cermet powder for comparison with dry-ball milled CND50 were explored. The first method, non-aqueous spray drying, was performed at Niro Inc. used a 40/60 (wt %) ethanol/toluene solvent and three binder systems; polyvinyl butyral (B79), ethylcellulose (Ethocel), and hydroxypropylcellulose (Klucel). Due to the nature of small spray-dry systems, an excess amount of fines was present in the granulated powder, which may have contributed to the low angles of repose (68 to 78). This is a moderate increase in 5 flowability as standard dry-ball milled powder possesses an angle of repose of 79-89. Mist granulated powders were produced with a tert-butanol solvent and polyvinyl butyral binder system. The angles of repose were more promising (28). More investigation into the mist granulation method is required. Also, aqueous spray drying may be possible with cermet and should be explored. Compaction of all granulated powders is much closer to a proven pressing powder (Sandi94 - angle of repose 29) which should allow cermet to be pressed to near net shape where die filling is difficult for non-flowing powders.4.Microstructure Characterization An analytical technique was developed to numerically characterize microstructures in terms of molybdenum dispersion, homogeneity, and percolation indices. This technique was applied to dry-ball-milled samples of various ball-milling times (0.5 to 20 hours). Significant change in the microstructure could be seen with milling time. Increased milling time caused agglomeration of molybdenum particles, increasing the percolation index, whereas short milling times promoted higher dispersion indices. This phenomenon is contrary to conventional understanding of mixing. However, conventional ball milling does not usually incorporate granules with binder and separate particles. This discrepancy may explain the odd mixing behavior. It is important to note that the high percolation index possessed by long ball mill times showed lower electrical resistance than low-percolation-index microstructures. However, machinability of high percolation, low-dispersion-index microstructures were poor as compared to microstructures with high dispersion indices and moderate percolation indices. This trade-off between dispersion and percolation (at constant molybdenum levels) suggests that microstructures can be achieved that posses good mechanical and electrical properties. Coincidentally, microstructures that satisfy this condition are produced by the standard dry-ball-milled CND50 (4 hour ball mill time). The performance and sensitivity of the microstructure characterization technique should be evaluated, specifically for electrical conductivity. Processing techniques to decrease the percolation index (lowering molybdenum content, excess ball milling, 6 larger molybdenum particles, etc.) should be employed to determine the point where cermet is not conductive or falls below electrical conduction specifications.7« less

Prospective clinical study comparing intraligamentary anesthesia and inferior alveolar nerve block for extraction of posterior mandibular teeth.

PubMed

Kämmerer, P W; Adubae, A; Buttchereit, I; Thiem, D G E; Daubländer, M; Frerich, B

2018-04-01

The aim of the study was to compare the efficacy of intraligamentary anesthesia (ILA) with conventional inferior alveolar nerve block (IANB) for extraction of mandibular posterior teeth. In a prospective clinical trial, a total of 301 mandibular posterior teeth were extracted in 266 patients. Randomization was conducted into those who received ILA (patients n = 98; teeth n = 105) and those who received IANB (patient n = 140; teeth n = 140). Twenty-eight patients were subjected to bilateral mandibular dental extractions and received both ILA und IANB (teeth n = 56 (ILA n = 28; IANB n = 28)). The primary objective was to evaluate the differences in pain during injection, in pain during tooth extraction (numeric rating scale (NRS)), and in anesthetic quality (complete/sufficient vs. insufficient/no effect). Differences in latency time, amount of anesthetic solution, need for second injection, and duration of local numbness as well as in the incidence of dry socket were assessed. ILA had significant lower pain of injection (p < 0.001), shorter latency time (p < 0.001), and shorter duration of local numbness (p < 0.001) and required lesser amount of local anesthetic solution (p < 0.001) together with a similar anesthetic quality (p = 0.082) compared to IANB. Concerning pain during extraction (p = 0.211), frequency of second injection (p = 0.197), and incidence of dry socket (p = 0.178), no significant differences were detected. ILA fulfills the requirements of a minimal invasive and patient-friendly local anesthetic technique. In accordance, it represents a safe and reliable alternative to IANB for extraction of mandibular posterior teeth. ILA can be recommended for routine dental extractions.

An Examination of Extreme Fire Behavior and its Impact on Smoke Injection Altitude using Remote Sensing and Meteorological Data

NASA Astrophysics Data System (ADS)

Peterson, D. A.; Hyer, E. J.; Campbell, J. R.; Fromm, M. D.; Hair, J. W.; Butler, C. F.; Fenn, M. A.

2014-12-01

A variety of regional smoke forecasting applications are currently available to identify air quality, visibility, and societal impacts during large fire events. However, these systems typically assume persistent fire activity, and therefore can have large errors before, during, and after short-term periods of extreme fire behavior. This study employs a wide variety of ground, airborne, and satellite observations, including data collected during a major NASA airborne and field campaign, to examine the conditions required for both extreme spread and pyrocumulonimbus (pyroCb) development. Results highlight the importance of upper-level and nocturnal meteorology, as well as the limitations of traditional fire weather indices. Increasing values of fire radiative power (FRP) at the pixel and sub-pixel level are shown to systematically correspond to higher altitude smoke plumes, and an increased probability of injection above the boundary layer. Lidar data collected during the 2013 Rim Fire, one of the most severe fire events in California's history, show that high FRP observed during extreme spread can facilitate long-distance smoke transport, but fails to loft smoke to the altitude of a large pyroCb. The most extreme fire spread was also observed on days without pyroCb activity or significant regional convection. By incorporating additional fire events across North America, conflicting hypotheses surrounding the primary source of moisture during pyroCb development are examined. The majority of large pyroCbs, and therefore the highest direct injection altitude of smoke particles, is shown to occur with conditions very similar to those that produce dry thunderstorms. The current suite of automated forecasting applications predict only general trends in fire behavior, and specifically do not predict (1) extreme fire spread events and (2) injection of smoke to high altitudes. While (1) and (2) are related, results show that they are not predicted by the same set of conditions and variables. The combination of meteorology from numerical forecast models and satellite observations exhibits great potential for improving regional forecasts of fire behavior and smoke production in automated systems, especially in remote areas where detailed observations are unavailable

Geothermal Program Review XI: proceedings. Geothermal Energy - The Environmental Responsible Energy Technology for the Nineties

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1993-10-01

These proceedings contain papers pertaining to current research and development of geothermal energy in the USA. The seven sections of the document are: Overview, The Geysers, Exploration and Reservoir Characterization, Drilling, Energy Conversion, Advanced Systems, and Potpourri. The Overview presents current DOE energy policy and industry perspectives. Reservoir studies, injection, and seismic monitoring are reported for the geysers geothermal field. Aspects of geology, geochemistry and models of geothermal exploration are described. The Drilling section contains information on lost circulation, memory logging tools, and slim-hole drilling. Topics considered in energy conversion are efforts at NREL, condensation on turbines and geothermal materials.more » Advanced Systems include hot dry rock studies and Fenton Hill flow testing. The Potpourri section concludes the proceedings with reports on low-temperature resources, market analysis, brines, waste treatment biotechnology, and Bonneville Power Administration activities. Selected papers have been indexed separately for inclusion in the Energy Science and Technology Database.« less

30 CFR 36.22 - Fuel-injection system.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Fuel-injection system. 36.22 Section 36.22... EQUIPMENT Construction and Design Requirements § 36.22 Fuel-injection system. This system shall be so.... Provision shall be made for convenient adjustment of the maximum fuel-injection rate to that required for...

30 CFR 36.22 - Fuel-injection system.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Fuel-injection system. 36.22 Section 36.22... EQUIPMENT Construction and Design Requirements § 36.22 Fuel-injection system. This system shall be so.... Provision shall be made for convenient adjustment of the maximum fuel-injection rate to that required for...

30 CFR 36.22 - Fuel-injection system.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Fuel-injection system. 36.22 Section 36.22... EQUIPMENT Construction and Design Requirements § 36.22 Fuel-injection system. This system shall be so.... Provision shall be made for convenient adjustment of the maximum fuel-injection rate to that required for...

30 CFR 36.22 - Fuel-injection system.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Fuel-injection system. 36.22 Section 36.22... EQUIPMENT Construction and Design Requirements § 36.22 Fuel-injection system. This system shall be so.... Provision shall be made for convenient adjustment of the maximum fuel-injection rate to that required for...

30 CFR 36.22 - Fuel-injection system.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Fuel-injection system. 36.22 Section 36.22... EQUIPMENT Construction and Design Requirements § 36.22 Fuel-injection system. This system shall be so.... Provision shall be made for convenient adjustment of the maximum fuel-injection rate to that required for...

Gelcasting compositions having improved drying characteristics and machinability

DOEpatents

Janney, Mark A.; Walls, Claudia A. H.

2001-01-01

A gelcasting composition has improved drying behavior, machinability and shelf life in the dried and unfired state. The composition includes an inorganic powder, solvent, monomer system soluble in the solvent, an initiator system for polymerizing the monomer system, and a plasticizer soluble in the solvent. Dispersants and other processing aides to control slurry properties can be added. The plasticizer imparts an ability to dry thick section parts, to store samples in the dried state without cracking under conditions of varying relative humidity, and to machine dry gelcast parts without cracking or chipping. A method of making gelcast parts is also disclosed.

Anti-inflammatory activity of dried flower extracts of Aegle marmelos in Wistar rats.

PubMed

Kumari, K D K P; Weerakoon, T C S; Handunnetti, S M; Samarasinghe, K; Suresh, T S

2014-02-12

Almost all part of the plant Aegle marmelos (Bael tree) has been used in the traditional medicine systems of Asian countries to treat various diseases over many centuries. The water extract of the dried flowers of Aegle marmelos is a commonly used beverage among Sri Lankan population in rural areas. Although extensive investigations done on many parts of the plant there are no experimental data available on the extracts of flowers. Anti-inflammatory effect of the water extract of dried flowers of Aegle marmelos (WEAM) was evaluated in the present study. The anti-inflammatory effect of the WEAM was evaluated by inhibition of the rat paw oedema, induced by carrageenan. The mechanism of the anti-inflammatory effect was assessed by the inhibition of production of nitric oxide (NO) by rat peritoneal cells, infiltration of rat peritoneal cells, anti-histamine effect, membrane stabilization activity, the antioxidant capacity and inhibition of lipid peroxidation by the WEAM. The maximum percentage inhibition of paw oedema was exhibited by the dose of 200 mg/kg at 2 h. The WEAM showed a significant increment of rat peritoneal cell infiltration, inhibition of NO production by rat peritoneal cells and inhibition of wheal formation on the skin of the rat after injection of histamine. The WEAM protected the erythrocyte membrane from heat-induced lysis in a dose-dependent manner and showed a significant anti-oxidant effect and lipid peroxidation inhibition activity. The WEAM possesses significant anti-inflammatory effect by multiple mechanisms in Wistar rats. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Towards defect free EUVL reticles: carbon and particle removal by single dry cleaning process and pattern repair by HIM

NASA Astrophysics Data System (ADS)

Koster, N. B.; Molkenboer, F. T.; van Veldhoven, E.; Oostrom, S.

2011-04-01

We report on our findings on EUVL reticle contamination removal, inspection and repair. We show that carbon contamination can be removed without damage to the reticle by our plasma process. Also organic particles, simulated by PSL spheres, can be removed from both the surface of the absorber as well as from the bottom of the trenches. The particles shrink in size during the plasma treatment until they are vanished. The determination of the necessary cleaning time for PSL spheres was conducted on Ru coated samples and the final experiment was performed on our dummy reticle. Finally we show that the Helium Ion Microscope in combination with a Gas Injection System is capable of depositing additional lines and squares on the reticle with sufficient resolution for pattern repair.

Process for removing sulfur from sulfur-containing gases: high calcium fly-ash

DOEpatents

Rochelle, Gary T.; Chang, John C. S.

1991-01-01

The present disclosure relates to improved processes for treating hot sulfur-containing flue gas to remove sulfur therefrom. Processes in accordance with the present invention include preparing an aqueous slurry composed of a calcium alkali source and a source of reactive silica and/or alumina, heating the slurry to above-ambient temperatures for a period of time in order to facilitate the formation of sulfur-absorbing calcium silicates or aluminates, and treating the gas with the heat-treated slurry components. Examples disclosed herein demonstrate the utility of these processes in achieving improved sulfur-absorbing capabilities. Additionally, disclosure is provided which illustrates preferred configurations for employing the present processes both as a dry sorbent injection and for use in conjunction with a spray dryer and/or bagfilter. Retrofit application to existing systems is also addressed.

Successful respiratory immunization with dry powder live-attenuated measles virus vaccine in rhesus macaques

PubMed Central

Lin, Wen-Hsuan; Griffin, Diane E.; Rota, Paul A.; Papania, Mark; Cape, Stephen P.; Bennett, David; Quinn, Brian; Sievers, Robert E.; Shermer, Charles; Powell, Kenneth; Adams, Robert J.; Godin, Steven; Winston, Scott

2011-01-01

Measles remains an important cause of childhood mortality worldwide. Sustained high vaccination coverage is the key to preventing measles deaths. Because measles vaccine is delivered by injection, hurdles to high coverage include the need for trained medical personnel and a cold chain, waste of vaccine in multidose vials and risks associated with needle use and disposal. Respiratory vaccine delivery could lower these barriers and facilitate sustained high coverage. We developed a novel single unit dose, dry powder live-attenuated measles vaccine (MVDP) for respiratory delivery without reconstitution. We tested the immunogenicity and protective efficacy in rhesus macaques of one dose of MVDP delivered either with a mask or directly intranasal with two dry powder inhalers, PuffHaler and BD Solovent. MVDP induced robust measles virus (MeV)-specific humoral and T-cell responses, without adverse effects, which completely protected the macaques from infection with wild-type MeV more than one year later. Respiratory delivery of MVDP was safe and effective and could aid in measles control. PMID:21282608

Heat and Mass Transfer Model in Freeze-Dried Medium

NASA Astrophysics Data System (ADS)

Alfat, Sayahdin; Purqon, Acep

2017-07-01

There are big problems in agriculture sector every year. One of the major problems is abundance of agricultural product during the peak of harvest season that is not matched by an increase in demand of agricultural product by consumers, this causes a wasted agricultural products. Alternative way was food preservation by freeze dried method. This method was already using heat transfer through conduction and convection to reduce water quality in the food. The main objective of this research was to design a model heat and mass transfer in freeze-dried medium. We had two steps in this research, the first step was design of medium as the heat injection site and the second was simulate heat and mass transfer of the product. During simulation process, we use physical property of some agriculture product. The result will show how temperature and moisture distribution every second. The method of research use finite element method (FEM) and will be illustrated in three dimensional.

Successful respiratory immunization with dry powder live-attenuated measles virus vaccine in rhesus macaques.

PubMed

Lin, Wen-Hsuan; Griffin, Diane E; Rota, Paul A; Papania, Mark; Cape, Stephen P; Bennett, David; Quinn, Brian; Sievers, Robert E; Shermer, Charles; Powell, Kenneth; Adams, Robert J; Godin, Steven; Winston, Scott

2011-02-15

Measles remains an important cause of childhood mortality worldwide. Sustained high vaccination coverage is the key to preventing measles deaths. Because measles vaccine is delivered by injection, hurdles to high coverage include the need for trained medical personnel and a cold chain, waste of vaccine in multidose vials and risks associated with needle use and disposal. Respiratory vaccine delivery could lower these barriers and facilitate sustained high coverage. We developed a novel single unit dose, dry powder live-attenuated measles vaccine (MVDP) for respiratory delivery without reconstitution. We tested the immunogenicity and protective efficacy in rhesus macaques of one dose of MVDP delivered either with a mask or directly intranasal with two dry powder inhalers, PuffHaler and BD Solovent. MVDP induced robust measles virus (MeV)-specific humoral and T-cell responses, without adverse effects, which completely protected the macaques from infection with wild-type MeV more than one year later. Respiratory delivery of MVDP was safe and effective and could aid in measles control.

Evaluation of food drying with air dehumidification system: a short review

NASA Astrophysics Data System (ADS)

Djaeni, M.; Utari, F. D.; Sasongko, S. B.; Kumoro, A. C.

2018-01-01

Energy efficient drying for food and agriculture products resulting high quality products has been an important issue. Currently, about 50% of total energy for postharvest treatment was used for drying. This paper presents the evaluation of new approach namely air dehumidification system with zeolite for food drying. Zeolite is a material having affinity to water in which reduced the moisture in air. With low moisture content and relative humidity, the air can improve driving force for drying even at low temperature. Thus, the energy efficiency can be potentially enhanced and the product quality can be well retained. For proving the hypothesis, the paddy and onion have been dried using dehumidified air. As performance indicators, the drying time, product quality, and heat efficiency were evaluated. Results indicated that the drying with zeolite improved the performances significantly. At operating temperature ranging 50 - 60°C, the efficiency of drying system can reach 75% with reasonable product quality.

Compressive, diametral tensile and biaxial flexural strength of cutting-edge calcium phosphate cements.

PubMed

Luo, Jun; Ajaxon, Ingrid; Ginebra, Maria Pau; Engqvist, Håkan; Persson, Cecilia

2016-07-01

Calcium phosphate cements (CPCs) are widely used in bone repair. Currently there are two main types of CPCs, brushite and apatite. The aim of this project was to evaluate the mechanical properties of particularly promising experimental brushite and apatite formulations in comparison to commercially available brushite- and apatite-based cements (chronOS(™) Inject and Norian(®) SRS(®), respectively), and in particular evaluate the diametral tensile strength and biaxial flexural strength of these cements in both wet and dry conditions for the first time. The cements׳ porosity and their compressive, diametral tensile and biaxial flexural strength were tested in wet (or moist) and dry conditions. The surface morphology was characterized by scanning electron microscopy. Phase composition was assessed with X-ray diffraction. It was found that the novel experimental cements showed better mechanical properties than the commercially available cements, in all loading scenarios. The highest compressive strength (57.2±6.5MPa before drying and 69.5±6.0MPa after drying) was found for the experimental brushite cement. This cement also showed the highest wet diametral tensile strength (10.0±0.8MPa) and wet biaxial flexural strength (30.7±1.8MPa). It was also the cement that presented the lowest porosity (approx. 12%). The influence of water content was found to depend on cement type, with some cements showing higher mechanical properties after drying and some no difference after drying. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Fundamental Studies of Clay and Clay-rich Mineral Reactions with H2O-CO2 Fluids: Application to Geological Carbon Dioxide Sequestration

NASA Astrophysics Data System (ADS)

Chizmeshya, A. V.

2016-12-01

Geological sequestration is currently being actively developed as a near-term, large-scale carbon sequestration technology in which supercritical carbon dioxide (scCO2) is injected below-ground into saline aquifers, depleted and existing oil and gas reservoir. Implementation strongly depends on the specific geological profile of each candidate injection site. Caprock formations that contain swellable clay minerals are of particular concern, since interaction with injected CO2 may produce complex local structural effects related to shrinkage, desiccation, and plastic response leading to CO2 escape. The current knowledge-base on rock-brine-CO2 interactions often relies on semi-empirical geochemical modeling and autoclave experiments, which necessitate quenching (de-gassing) to ambient conditions for characterization. To avoid these effects we used a moissanite-based microreaction system (Diefenbacher, J et al Rev. Mod. Inst., 76 15103 (2005)) which enables in situ synchrotron characterization of interactions under constant CO2 activity. Synchrotron studies were performed at the GSECARS sector of the Argonne National Lab APS to systematically determine the response of representative Ca- and Na-montmorillonites (STx-1, SWy-1) clays to dry/wet scCO2 (H2O-rich) fluids at T and P encountered in typical aquifers. Our main findings for hydrated STx-1 are that desiccation occurs spontaneously on the scale of minutes-hours over a wide range of conditions in dry scCO2 via release of H2O with volume changes as large as 19% in relation to the initial volume. Desiccation was not observed in wet scCO2, or in corresponding saline solutions containing 1-3 M NaCl, but quenching to ambient conditions from low-pressures leads to re-hydration in STx-1 suggesting a pressure-dependent diffusion barrier for H2O from the clay into bulk scCO2. Similar desiccation transitions with smaller volume changes of 5-9% were also observed in SWy-1 at P 140 atm and T 40 C. At high pressures ( 200 atm) minor lamellar expansion was sometimes found in STx-1 suggesting the possible intercalation of CO2. Our diffraction studies reveal an intriguing relationship between the critical dehydration temperatures and pressures, with bounds defined by critical properties of CO2.

An Innovative Needle-free Injection System: Comparison to 1 ml Standard Subcutaneous Injection.

PubMed

Kojic, Nikola; Goyal, Pragun; Lou, Cheryl Hamer; Corwin, Michael J

2017-11-01

A needle-free delivery system may lead to improved satisfaction and compliance, as well as reduced anxiety among patients requiring frequent or ongoing injections. This report describes a first-in-man assessment comparing Portal Instruments' innovative needle-free injection system with subcutaneous injections using a 27G needle. Forty healthy volunteer participants each received a total of four injections of 1.0 mL sterile saline solution, two with a standard subcutaneous injection using a 27G needle, and two using the Portal injection system. Perception of pain was measured using a 100-mm visual analog scale (VAS). Injection site reactions were assessed at 2 min and at 20-30 min after each injection. Follow-up contact was made 24-48 h after the injections. Subject preference regarding injection type was also assessed. VAS pain scores at Portal injection sites met the criteria to be considered non-inferior to the pain reported at 27G needle injection sites (i.e., upper 95% confidence bound less than +5 mm). Based on a mixed effects model, at time 0, accounting for potential confounding variables, the adjusted difference in VAS scores indicated that Portal injections were 6.5 mm lower than the 27G needle injections (95% CI -10.5, -2.5). No clinically important adverse events were noted. Portal injections were preferred by 24 (60%) of the subjects (P = 0.0015). As an early step in the development of this new needle-free delivery system, the current study has shown that a 1.0-mL saline injection can be given with less pain reported than a standard subcutaneous injection using a 27G needle.

Risk Analysis of On-Orbit Spacecraft Refueling Concepts

NASA Technical Reports Server (NTRS)

Cirillo, William M.; Stromgren, Chel; Cates, Grant R.

2010-01-01

On-orbit refueling of spacecraft has been proposed as an alternative to the exclusive use of Heavy-lift Launch Vehicles to enable human exploration beyond Low Earth Orbit (LEO). In these scenarios, beyond LEO spacecraft are launched dry (without propellant) or partially dry into orbit, using smaller or fewer element launch vehicles. Propellant is then launched into LEO on separate launch vehicles and transferred to the spacecraft. Refueling concepts are potentially attractive because they reduce the maximum individual payload that must be placed in Earth orbit. However, these types of approaches add significant complexity to mission operations and introduce more uncertainty and opportunities for failure to the mission. In order to evaluate these complex scenarios, the authors developed a Monte Carlo based discrete-event model that simulates the operational risks involved with such strategies, including launch processing delays, transportation system failures, and onorbit element lifetimes. This paper describes the methodology used to simulate the mission risks for refueling concepts, the strategies that were evaluated, and the results of the investigation. The results of the investigation show that scenarios that employ refueling concepts will likely have to include long launch and assembly timelines, as well as the use of spare tanker launch vehicles, in order to achieve high levels of mission success through Trans Lunar Injection.

CEC-atmospheric pressure ionization MS of pesticides using a surfactant-bound monolithic column.

PubMed

Gu, Congying; Shamsi, Shahab A

2010-04-01

A surfactant bound poly (11-acrylaminoundecanoic acid-ethylene dimethacrylate) monolithic column was simply prepared by in situ co-polymerization of 11-acrylaminoundecanoic acid and ethylene dimethacrylate with 1-propanol, 1,4-butanediol and water as porogens in 100 microm id fused-silica capillary in one step. This column was used in CEC-atmospheric pressure photoionization (APPI)-MS system for separation and detection of N-methylcarbamates pesticides. Numerous parameters are optimized for CEC-APPI-MS. After evaluation of the mobile phase composition, sheath liquid composition and the monolithic capillary outlet position, a fractional factorial design was selected as a screening procedure to identify factors of ionization source parameters, such as sheath liquid flow rate, drying gas flow rate, drying gas temperature, nebulizing gas pressure, vaporizer temperature and capillary voltage, which significantly influence APPI-MS sensitivity. A face-centered central composite design was further utilized to optimize the most significant parameters and predict the best sensitivity. Under optimized conditions, S/Ns around 78 were achieved for an injection of 100 ng/mL of each pesticide. Finally, this CEC-APPI-MS method was successfully applied to the analysis of nine N-methylcarbamates in spiked apple juice sample after solid phase extraction with recoveries in the range of 65-109%.

Estimation of perimortal percent carboxy-heme in nonstandard postmortem specimens using analysis of carbon monoxide by GC/MS and iron by flame atomic absorption spectrophotometry.

PubMed

Middleberg, R A; Easterling, D E; Zelonis, S F; Rieders, F; Rieders, M F

1993-01-01

In decomposed, formalin-fixed, embalmed, exhumed, and some fire-dried cases in which normal blood is unavailable, the usual methods for determination of carboxyhemoglobin saturation frequently fail. To address these specimens, a method utilizing both gas chromatography/mass spectrometric (GC/MS) determination of carbon monoxide (CO) and flame atomic absorption spectrophotometry (FAAS) determination of iron (Fe), in the same specimen, was developed. The method is reported here, along with its application to seven pertinent forsensic death investigations. The CO analytical methodology involves acid liberation of the gas from the specimen aliquot in a headspace vial. After heating and equilibrating, a sample of the headspace vapor is injected into the GC/MS system with a gastight syringe. Quantitation is achieved by standard addition comparison utilizing the ideal gas law equation. Iron is quantified by FAAS analysis of the same aliquot used for the CO determination, following nitric acid digestion. The concentration is determined by comparison to a standard curve. A formula for determining the minimum percent carboxy-heme saturation was derived by using the ratio of the amount of CO to the amount of Fe in the aliquot analyzed. Tissue types analyzed include spleen, liver, muscle, dried blood, and unspecified decomposed tissue.

Environmental and Economic Performance of Commercial-scale Solar Photovoltaic Systems: A Field Study of Complex Energy Systems at the Desert Research Institute (DRI)

NASA Astrophysics Data System (ADS)

Liu, X.

2014-12-01

Solar photovoltaic (PV) systems are being aggressively deployed at residential, commercial, and utility scales to complement power generation from conventional sources. This is motivated both by the desire to reduce carbon footprints and by policy-driven financial incentives. Although several life cycle analyses (LCA) have investigated environmental impacts and energy payback times of solar PV systems, most results are based on hypothetical systems rather than actual, deployed systems that can provide measured performance data. Over the past five years, Desert Research Institute (DRI) in Nevada has installed eight solar PV systems of scales from 3 to 1000 kW, the sum of which supply approximately 40% of the total power use at DRI's Reno and Las Vegas campuses. The goal of this work is to explore greenhouse gas (GHG) impacts and examine the economic performance of DRI's PV systems by developing and applying a comprehensive LCA and techno-economic (TEA) model. This model is built using data appropriate for each type of panel used in the DRI systems. Power output is modeled using the National Renewable Energy Laboratory (NREL) model PVWatts. The performance of PVWatts is verified by the actual measurements from DRI's PV systems. Several environmental and economic metrics are quantified for the DRI systems, including life cycle GHG emissions and energy return. GHG results are compared with Nevada grid-based electricity. Initial results indicate that DRI's solar-derived electricity offers clear GHG benefits compared to conventional grid electricity. DRI's eight systems have GHG intensity values of 29-56 gCO2e/kWh, as compared to the GHG intensity of 212 gCO2e/kWh of national average grid power. The major source of impacts (82-92% of the total) is the upstream life cycle burden of manufacturing PV panels, which are made of either mono-crystalline or multi-crystalline silicon. Given the same type of PV panel, GHG intensity decreases as the scale of the system increases. Energy payback times of DRI's solar PV systems range from 0.5 to 1.5 years. The cost payback time for the DRI PV systems and the cost per ton of CO2 avoided by replacing Nevada-specific electrical power will be determined. The sensitivity of these environmental and economic impacts with respect to specific model parameters is being investigated.

Dynamic Ocular Surface and Lacrimal Gland Changes Induced in Experimental Murine Dry Eye

PubMed Central

Xiao, Bing; Wang, Yu; Reinach, Peter S.; Ren, Yueping; Li, Jinyang; Hua, Shanshan; Lu, Huihui; Chen, Wei

2015-01-01

Dry eye disease can be a consequence of lacrimal gland insufficiency in Sjögren’s Syndrome or increased tear film evaporation despite normal lacrimal gland function. To determine if there is a correlation between severity effects in these models and underlying pathophysiological responses, we compared the time dependent changes in each of these parameters that occur during a 6 week period. Dry eye was induced in 6-week-old female C57BL/6 mice by exposing them to an Intelligently Controlled Environmental System (ICES). Sixty mice were housed in ICES for 1, 2, 4 and 6 weeks respectively. Twelve were raised in normal environment and received subcutaneous injections of scopolamine hydrobromide (SCOP) 3 times daily for 5 days. Another sixty mice were housed in a normal environment and received no treatment. Corneal fluorescein staining along with corneal MMP-9 and caspase-3 level measurements were performed in parallel with the TUNEL assay. Interleukin-17(IL-17), IL-23, IL-6, IL-1, TNF-α, IFN-γ and TGF-β2 levels were estimated by real-time PCR measurements of conjunctival and lacrimal gland samples (LGs). Immunohistochemistry of excised LGs along with flow cytometry in cervical lymph nodes evaluated immune cell infiltration. Light and transmission electron microscopy studies evaluated LGs cytoarchitectural changes. ICES induced corneal epithelial destruction and apoptosis peaked at 2 weeks and kept stable in the following 4 weeks. In the ICES group, lacrimal gland proinflammatory cytokine level increases were much lower than those in the SCOP group. In accord with the lower proinflammatory cytokine levels, in the ICES group, lacrimal gland cytosolic vesicular density and size exceeded that in the SCOP group. ICES and SCOP induced murine dry eye effects became progressively more severe over a two week period. Subsequently, the disease process stabilized for the next four weeks. ICES induced local effects in the ocular surface, but failed to elicit lacrimal gland inflammation and cytoarchitectural changes, which accounts for less dry eye severity in the ICES model than that in the SCOP model. PMID:25590134

Dynamic ocular surface and lacrimal gland changes induced in experimental murine dry eye.

PubMed

Xiao, Bing; Wang, Yu; Reinach, Peter S; Ren, Yueping; Li, Jinyang; Hua, Shanshan; Lu, Huihui; Chen, Wei

2015-01-01

Dry eye disease can be a consequence of lacrimal gland insufficiency in Sjögren's Syndrome or increased tear film evaporation despite normal lacrimal gland function. To determine if there is a correlation between severity effects in these models and underlying pathophysiological responses, we compared the time dependent changes in each of these parameters that occur during a 6 week period. Dry eye was induced in 6-week-old female C57BL/6 mice by exposing them to an Intelligently Controlled Environmental System (ICES). Sixty mice were housed in ICES for 1, 2, 4 and 6 weeks respectively. Twelve were raised in normal environment and received subcutaneous injections of scopolamine hydrobromide (SCOP) 3 times daily for 5 days. Another sixty mice were housed in a normal environment and received no treatment. Corneal fluorescein staining along with corneal MMP-9 and caspase-3 level measurements were performed in parallel with the TUNEL assay. Interleukin-17(IL-17), IL-23, IL-6, IL-1, TNF-α, IFN-γ and TGF-β2 levels were estimated by real-time PCR measurements of conjunctival and lacrimal gland samples (LGs). Immunohistochemistry of excised LGs along with flow cytometry in cervical lymph nodes evaluated immune cell infiltration. Light and transmission electron microscopy studies evaluated LGs cytoarchitectural changes. ICES induced corneal epithelial destruction and apoptosis peaked at 2 weeks and kept stable in the following 4 weeks. In the ICES group, lacrimal gland proinflammatory cytokine level increases were much lower than those in the SCOP group. In accord with the lower proinflammatory cytokine levels, in the ICES group, lacrimal gland cytosolic vesicular density and size exceeded that in the SCOP group. ICES and SCOP induced murine dry eye effects became progressively more severe over a two week period. Subsequently, the disease process stabilized for the next four weeks. ICES induced local effects in the ocular surface, but failed to elicit lacrimal gland inflammation and cytoarchitectural changes, which accounts for less dry eye severity in the ICES model than that in the SCOP model.

Effect of Fumigation on Rotylenchulus reniformis Population Density Through Subsurface Drip Irrigation Located Every Other Furrow.

PubMed

Wheeler, T A; Porter, D O; Archer, D; Mullinix, B G

2008-09-01

Plots naturally infested with Rotylenchulus reniformis were sampled in the spring of 2006 and 2007 at depths of 15 and 30 cm in the bed, furrow over the drip tape, and "dry" furrow, and at approximately 40 to 45 cm depth in the bed and dry furrow. Then, 1,3-dichloropropene (Telone EC) was injected into the subsurface drip irrigation at 46 kg a.i./ha, and 3 to 4 weeks later the plots were resampled and assayed for nematodes. The transformed values for nematode population density (IvLRr) before fumigation were higher at 30 and 40 cm depths than at a 15 cm depth. IvLRr before fumigation was higher in the soil over the drip lines than in the bed or dry furrow and was higher in the bed than the dry furrow. IvLRr was higher in the plots to be fumigated than the plots that were not to be fumigated for all depths and locations except at a 15 cm depth over the drip lines, where the values were similar. However, after fumigation, IvLRr was lower over the drip lines at a 30 cm depth in plots that were fumigated compared to samples in a similar location and depth that were not fumigated. There were no other location/depth combinations where the fumigation reduced IvLRr below that in the nonfumigated plots. Yield in 2006, which was a very hot and dry year, was predicted adequately (R(2) = 0.67) by a linear model based on the preplant population density of R. reniformis, with a very steep slope (-2.8 kg lint/ha per R. reniformis/100 cm(3) soil). However, no relationship between nematode density and yield was seen in 2007, which had cooler weather for most of the season. Yield was not significantly improved by fumigation through the drip irrigation system in either year compared to plots treated only with aldicarb (0.84 kg a.i./ha), indicating that the level of control with fumigation did not kill enough R. reniformis to be successful.

Fast method for simultaneous quantification of tamoxifen and metabolites in dried blood spots using an entry level LC-MS/MS system.

PubMed

Tré-Hardy, Marie; Capron, Arnaud; Antunes, Marina Venzon; Linden, Rafael; Wallemacq, Pierre

2016-11-01

The purpose of this study was to develop and validate a new liquid chromatography-tandem mass spectrometric (LC-MSMS) assay for the simultaneous quantification of tamoxifen (TAM) and its main therapeutically active metabolites, N-desmethyltamoxifen (NDT), 4-hydroxytamoxifen (4HT) and endoxifen (END) in dried blood spots. Ultrasound assisted methanolic extraction was used for TAM and metabolites extraction from dried blood spot. After evaporation and methanol reconstitution, the extract was injected into a LC-MSMS system. Reversed phase chromatography was performed on a C18 grafted column in gradient mode. TAM, metabolites, and internal standard (diazepam-d 5 ; IS) were identified in positive electrospray ionization mode using m/z transition of 372.5>72.1 (TAM); 374.23>58.10 (END); 358.27>58.10 (NDT); 388.23>44.80 (4HT) and 290.00>198.00 (IS). Total analytical run time was 6.5min. Assay was linear from 1 to 500ng/mL for all substances and presented intra and inter-assay precision and accuracy <15%. TAM, NDT, 4HT and END limits of quantification and detection were of 1 and 0.5ng/mL; 1 and 3ng/mL; 1.7 and 3ng/mL; 0.6 and 2ng/mL, respectively. Recovery ranged from 83.8 to 96.3% with matrix effect ranged from 4.3 to 29.8% for TAM and its metabolites. Hematocrit value ≤40% appeared to negatively influence accuracy of the method. In conclusion, the method described here is somewhat accessible, relatively fast, sensitive and selective with no interference. This assay might be used to investigate the level of TAM and its metabolites in DBS for therapeutic drug monitoring purposes. Copyright © 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

7 CFR 58.220 - Drying systems.

Code of Federal Regulations, 2012 CFR

2012-01-01

... filter system shall comply with the applicable requirements of the 3-A Accepted Practices for Milk and Milk Products Spray Drying Systems. The filtering system shall be cleaned or component parts replaced... shall comply with the requirements of the 3-A Accepted Practices for Milk and Milk Products Spray Drying...

7 CFR 58.220 - Drying systems.

Code of Federal Regulations, 2011 CFR

2011-01-01

... filter system shall comply with the applicable requirements of the 3-A Accepted Practices for Milk and Milk Products Spray Drying Systems. The filtering system shall be cleaned or component parts replaced... shall comply with the requirements of the 3-A Accepted Practices for Milk and Milk Products Spray Drying...

7 CFR 58.220 - Drying systems.

Code of Federal Regulations, 2013 CFR

2013-01-01

... filter system shall comply with the applicable requirements of the 3-A Accepted Practices for Milk and Milk Products Spray Drying Systems. The filtering system shall be cleaned or component parts replaced... shall comply with the requirements of the 3-A Accepted Practices for Milk and Milk Products Spray Drying...

7 CFR 58.220 - Drying systems.

Code of Federal Regulations, 2014 CFR

2014-01-01

... filter system shall comply with the applicable requirements of the 3-A Accepted Practices for Milk and Milk Products Spray Drying Systems. The filtering system shall be cleaned or component parts replaced... shall comply with the requirements of the 3-A Accepted Practices for Milk and Milk Products Spray Drying...

30 CFR 75.1101-12 - Equivalent dry-pipe system.

Code of Federal Regulations, 2010 CFR

2010-07-01

... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Fire Protection § 75.1101-12 Equivalent dry-pipe system. Where water sprinkler systems are installed to protect main and secondary belt... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Equivalent dry-pipe system. 75.1101-12 Section...

30 CFR 75.1101-12 - Equivalent dry-pipe system.

Code of Federal Regulations, 2011 CFR

2011-07-01

... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Fire Protection § 75.1101-12 Equivalent dry-pipe system. Where water sprinkler systems are installed to protect main and secondary belt... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Equivalent dry-pipe system. 75.1101-12 Section...

30 CFR 75.1101-12 - Equivalent dry-pipe system.

Code of Federal Regulations, 2012 CFR

2012-07-01

... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Fire Protection § 75.1101-12 Equivalent dry-pipe system. Where water sprinkler systems are installed to protect main and secondary belt... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Equivalent dry-pipe system. 75.1101-12 Section...

30 CFR 75.1101-12 - Equivalent dry-pipe system.

Code of Federal Regulations, 2013 CFR

2013-07-01

... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Fire Protection § 75.1101-12 Equivalent dry-pipe system. Where water sprinkler systems are installed to protect main and secondary belt... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Equivalent dry-pipe system. 75.1101-12 Section...

30 CFR 75.1101-12 - Equivalent dry-pipe system.

Code of Federal Regulations, 2014 CFR

2014-07-01

... SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Fire Protection § 75.1101-12 Equivalent dry-pipe system. Where water sprinkler systems are installed to protect main and secondary belt... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Equivalent dry-pipe system. 75.1101-12 Section...

The Determination of Several Spray Characteristics of a High-Speed Oil Engine Injection System with an Oscilloscope

NASA Technical Reports Server (NTRS)

Hicks, Chester W; Moore, Charles S

1928-01-01

An investigation was conducted to determine the injection lag, duration of injection, and spray start and cut-off characteristics of a fuel injection system operated on an engine and injecting fuel into the atmosphere.

Preparation and Characterization of Rivastigmine Transdermal Patch Based on Chitosan Microparticles.

PubMed

Sadeghi, Mohsen; Ganji, Fariba; Taghizadeh, Seyyed Mojtaba; Daraei, Bahram

2016-01-01

Here we report a novel approach for preparation of a 6-day transdermal drug delivery system (TDDS) as treatment for mild to moderate Alzheimer's disease. The spray drying method was used to prepare microparticles containing the anti-Alzheimer drug, Rivastigmine, in combination with the natural polymer, chitosan, for transdermal drug delivery applications. The content of the drug was determined by High Performance Liquid Chromatography (HPLC) method which was validated as per FDA guidelines. The morphology and size range of the microparticles were determined; and the effect of drug concentration in the solution injected into the spray dryer on the particles characterizations was studied. The stability of Rivastigmine at high temperature was confirmed using FTIR analysis as well as a validate HPLC assay. The obtained results show that the drug was stable at high temperatures with 7 to 42% loading in the microparticles, and the higher drug concentrations of the solution injected into the spray dryer resulted in increase of the drug loading, surface drug and microparticles distortion. The TDDS containing the microparticles was also prepared with microparticle to dry adhesive ratios of 5, 10 and 15% using acrylic adhesive. Based on adhesion properties of the patches, gained from the probe tack and the peel adhesion 180° tests, and the 15% patch by having more drug content per unit area of the patch, and still having similar adhesion properties was compared to the microparticles-free patch of 5.1% Rivastigmine salt (equivalent to the drug content of the 15% patch) from the permeation point of view by using Franz cell diffusion over 6 days. The drug permeation rate from the microparticle-free patch was slower than the 15% microparticles patch, which is the result of crystallization of Rivastigmine salt in the acrylic adhesive. The 6-day-prepared TDDS can be considered as an alternative for one-week application of 6 Exelon patches.

Seismic Signals Recorded During Artificial and Natural Karst Recharge Events

NASA Astrophysics Data System (ADS)

Bilek, S. L.; Luhmann, A. J.; Diniakos, R. S.; Morton, E.; Rinehart, A. J.; Alexander, E. C., Jr.; Alexander, S. C.; Larsen, M.; Green, J. A.

2016-12-01

Seismology has recently been used to explore a variety of surficial processes, including tracking landslides, glaciers, and river flow. The seismic data collected can provide useful information about these flow processes, particularly in areas where other flow-related observations are difficult to make. Underground flow through karst aquifers is an area of significant interest, where most of the flow occurs through preferential flow conduits whose locations are largely unknown. We investigate seismic signals generated by both controlled and natural recharge into a karst aquifer system located near Bear Spring, near Eyota, MN, USA. The controlled recharge experiments involved injecting a pool full of water ( 13,000 L) into a dry overflow spring, which then flowed underground until it was discharged at Bear Spring. The natural recharge fortuitously involved a large rainstorm that supplied over 2 inches of rain in a few hour period, which caused the overflow spring to start flowing and total discharge to increase from a background of 100 L/s to 300 L/s. These events were recorded by 12 seismometers (11 short period and 1 broadband with 500 Hz sampling rate) all placed within the 4500 m2 area containing the dry overflow spring and predicted underground flow path. Spectrograms for the artificial and natural recharge events show significant seismic energy over a broad frequency range (few to nearly 220 Hz) during the periods of artificial recharge, largely contained during the period of water injection. Stations closest to Bear Spring see peak seismic energy in the 20-30 Hz range during these tests, likely related to changes in the underground water flow. During the natural recharge event, we find much longer duration of seismic energy over the broad frequency range. We compare these seismic results with discharge measurements conducted during all tests at Bear Spring, as well as with rainfall measurements recorded for the natural recharge event.

Hot Dry Rock; Geothermal Energy

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1990-01-01

The commercial utilization of geothermal energy forms the basis of the largest renewable energy industry in the world. More than 5000 Mw of electrical power are currently in production from approximately 210 plants and 10 000 Mw thermal are used in direct use processes. The majority of these systems are located in the well defined geothermal generally associated with crustal plate boundaries or hot spots. The essential requirements of high subsurface temperature with huge volumes of exploitable fluids, coupled to environmental and market factors, limit the choice of suitable sites significantly. The Hot Dry Rock (HDR) concept at any depthmore » originally offered a dream of unlimited expansion for the geothermal industry by relaxing the location constraints by drilling deep enough to reach adequate temperatures. Now, after 20 years intensive work by international teams and expenditures of more than $250 million, it is vital to review the position of HDR in relation to the established geothermal industry. The HDR resource is merely a body of rock at elevated temperatures with insufficient fluids in place to enable the heat to be extracted without the need for injection wells. All of the major field experiments in HDR have shown that the natural fracture systems form the heat transfer surfaces and that it is these fractures that must be for geothermal systems producing from naturally fractured formations provide a basis for directing the forthcoming but, equally, they require accepting significant location constraints on HDR for the time being. This paper presents a model HDR system designed for commercial operations in the UK and uses production data from hydrothermal systems in Japan and the USA to demonstrate the reservoir performance requirements for viable operations. It is shown that these characteristics are not likely to be achieved in host rocks without stimulation processes. However, the long term goal of artificial geothermal systems developed by systematic engineering procedures at depth may still be attained if high temperature sites with extensive fracturing are developed or exploited. [DJE -2005]« less

Freeze-drying in novel container system: Characterization of heat and mass transfer in glass syringes.

PubMed

Patel, Sajal M; Pikal, Michael J

2010-07-01

This study is aimed at characterizing and understanding different modes of heat and mass transfer in glass syringes to develop a robust freeze-drying process. Two different holder systems were used to freeze-dry in syringes: an aluminum (Al) block and a plexiglass holder. The syringe heat transfer coefficient was characterized by a sublimation test using pure water. Mannitol and sucrose (5% w/v) were also freeze-dried, as model systems, in both the assemblies. Dry layer resistance was determined from manometric temperature measurement (MTM) and product temperature was measured using thermocouples, and was also determined from MTM. Further, freeze-drying process was also designed using Smart freeze-dryer to assess its application for freeze-drying in novel container systems. Heat and mass transfer in syringes were compared against the traditional container system (i.e., glass tubing vial). In the Al block, the heat transfer was via three modes: contact conduction, gas conduction, and radiation with gas conduction being the dominant mode of heat transfer. In the plexiglass holder, the heat transfer was mostly via radiation; convection was not involved. Also, MTM/Smart freeze-drying did work reasonably well for freeze-drying in syringes. When compared to tubing vials, product temperature decreases and hence drying time increases in syringes. (c) 2010 Wiley-Liss, Inc. and the American Pharmacists Association

Dynamic underground stripping: steam and electric heating for in situ decontamination of soils and groundwater

DOEpatents

Daily, W.D.; Ramirez, A.L.; Newmark, R.L.; Udell, K.; Buetnner, H.M.; Aines, R.D.

1995-09-12

A dynamic underground stripping process removes localized underground volatile organic compounds from heterogeneous soils and rock in a relatively short time. This method uses steam injection and electrical resistance heating to heat the contaminated underground area to increase the vapor pressure of the contaminants, thus speeding the process of contaminant removal and making the removal more complete. The injected steam passes through the more permeable sediments, distilling the organic contaminants, which are pumped to the surface. Large electrical currents are also applied to the contaminated area, which heat the impermeable subsurface layers that the steam has not penetrated. The condensed and vaporized contaminants are withdrawn by liquid pumping and vacuum extraction. The steam injection and electrical heating steps are repeated as necessary. Geophysical imaging methods can be used to map the boundary between the hot, dry, contamination-free underground zone and the cool, damp surrounding areas to help monitor the dynamic stripping process. 4 figs.

Dynamic underground stripping: steam and electric heating for in situ decontamination of soils and groundwater

DOEpatents

Daily, William D.; Ramirez, Abelardo L.; Newmark, Robin L.; Udell, Kent; Buetnner, Harley M.; Aines, Roger D.

1995-01-01

A dynamic underground stripping process removes localized underground volatile organic compounds from heterogeneous soils and rock in a relatively short time. This method uses steam injection and electrical resistance heating to heat the contaminated underground area to increase the vapor pressure of the contaminants, thus speeding the process of contaminant removal and making the removal more complete. The injected steam passes through the more permeable sediments, distilling the organic contaminants, which are pumped to the surface. Large electrical currents are also applied to the contaminated area, which heat the impermeable subsurface layers that the steam has not penetrated. The condensed and vaporized contaminants are withdrawn by liquid pumping and vacuum extraction. The steam injection and electrical heating steps are repeated as necessary. Geophysical imaging methods can be used to map the boundary between the hot, dry, contamination-free underground zone and the cool, damp surrounding areas to help monitor the dynamic stripping process.

Development and Assessment of Countermeasure Formulations for Treatment of Lung Injury Induced by Chlorine Inhalation

PubMed Central

Hoyle, Gary W.; Chen, Jing; Schlueter, Connie F.; Mo, Yiqun; Humphrey, David M.; Rawson, Greg; Niño, Joe A.; Carson, Kenneth H.

2016-01-01

Chlorine is a commonly used, reactive compound to which humans can be exposed via accidental or intentional release resulting in acute lung injury. Formulations of rolipram (a phosphodiesterase inhibitor), triptolide (a natural plant product with anti-inflammatory properties), and budesonide (a corticosteroid), either neat or in conjunction with poly(lactic:glycolic acid) (PLGA), were developed for treatment of chlorine-induced acute lung injury by intramuscular injection. Formulations were produced by spray-drying, which generated generally spherical microparticles that were suitable for intramuscular injection. Multiple parameters were varied to produce formulations with a wide range of in vitro release kinetics. Testing of selected formulations in chlorine-exposed mice demonstrated efficacy against key aspects of acute lung injury. The results show the feasibility of developing microencapsulated formulations that could be used to treat chlorine-induced acute lung injury by intramuscular injection, which represents a preferred route of administration in a mass casualty situation. PMID:26952014

Fusion energy with lasers, direct drive targets, and dry wall chambers

NASA Astrophysics Data System (ADS)

Sethian, J. D.; Friedman, M.; Lehmberg, R. H.; Myers, M.; Obenschain, S. P.; Giuliani, J.; Kepple, P.; Schmitt, A. J.; Colombant, D.; Gardner, J.; Hegeler, F.; Wolford, M.; Swanekamp, S. B.; Weidenheimer, D.; Welch, D.; Rose, D.; Payne, S.; Bibeau, C.; Baraymian, A.; Beach, R.; Schaffers, K.; Freitas, B.; Skulina, K.; Meier, W.; Latkowski, J.; Perkins, L. J.; Goodin, D.; Petzoldt, R.; Stephens, E.; Najmabadi, F.; Tillack, M.; Raffray, R.; Dragojlovic, Z.; Haynes, D.; Peterson, R.; Kulcinski, G.; Hoffer, J.; Geller, D.; Schroen, D.; Streit, J.; Olson, C.; Tanaka, T.; Renk, T.; Rochau, G.; Snead, L.; Ghoneim, N.; Lucas, G.

2003-12-01

A coordinated, focused effort is underway to develop Laser Inertial Fusion Energy. The key components are developed in concert with one another and the science and engineering issues are addressed concurrently. Recent advances include: target designs have been evaluated that show it could be possible to achieve the high gains (>100) needed for a practical fusion system.These designs feature a low-density CH foam that is wicked with solid DT and over-coated with a thin high-Z layer. These results have been verified with three independent one-dimensional codes, and are now being evaluated with two- and three-dimensional codes. Two types of lasers are under development: Krypton Fluoride (KrF) gas lasers and Diode Pumped Solid State Lasers (DPSSL). Both have recently achieved repetitive 'first light', and both have made progress in meeting the fusion energy requirements for durability, efficiency, and cost. This paper also presents the advances in development of chamber operating windows (target survival plus no wall erosion), final optics (aluminium at grazing incidence has high reflectivity and exceeds the required laser damage threshold), target fabrication (demonstration of smooth DT ice layers grown over foams, batch production of foam shells, and appropriate high-Z overcoats), and target injection (new facility for target injection and tracking studies).

Meso-Scale Wetting of Paper Towels

NASA Astrophysics Data System (ADS)

Abedsoltan, Hossein

In this study, a new experimental approach is proposed to investigate the absorption properties of some selected retail paper towels. The samples were selected from two important manufacturing processes, conventional wet pressing (CWP) considered value products, and through air drying (TAD) considered as high or premium products. The tested liquids were water, decane, dodecane, and tetradecane with the total volumes in micro-liter range. The method involves the point source injection of liquid with different volumetric flowrates, in the nano-liter per second range. The local site for injection was chosen arbitrarily on the sample surface. The absorption process was monitored and recorded as the liquid advances, with two distinct imaging system methods, infrared imaging and optical imaging. The microscopic images were analyzed to calculate the wetted regions during the absorption test, and the absorption diagrams were generated. These absorption diagrams were dissected to illustrate the absorption phenomenon, and the absorption properties of the samples. The local (regional) absorption rates were computed for Mardi Gras and Bounty Basic as the representative samples for CWP and TAD, respectively in order to be compared with the absorption capacity property of these two samples. Then, the absorption capacity property was chosen as an index factor to compare the absorption properties of all the tested paper towels.

An Apparatus for Measuring Rates of Discharge of a Fuel-Injection System

NASA Technical Reports Server (NTRS)

Dutee, Francis J

1941-01-01

A portable apparatus for rapidly determining rates of discharge of a fuel-injection system is described. Satisfactory operation of this apparatus with injection-pump speeds up to 2400 r.p.m was obtained. Rate-of-discharge tests were made with several cam-plunger-valve injection systems with long injection tubes. A check valve designed to reduce secondary discharges was tested. This check valve was operated with injection-pump speeds up to 2400 r.p.m without the occurrence of large secondary discharges.

Cowpeas and pinto beans: Performance and yields of candidate space crops in the laboratory biosphere closed ecological system

NASA Astrophysics Data System (ADS)

Nelson, M.; Dempster, W. F.; Allen, J. P.; Silverstone, S.; Alling, A.; van Thillo, M.

An experiment utilizing cowpeas ( Vigna unguiculata L.), pinto beans ( Phaseolus vulgaris L.) and Apogee ultra-dwarf wheat ( Triticum sativa L.) was conducted in the soil-based closed ecological facility, Laboratory Biosphere, from February to May 2005. The lighting regime was 13 h light/11 h dark at a light intensity of 960 μmol m -2 s -1, 45 mol m -2 day -1 supplied by high-pressure sodium lamps. The pinto beans and cowpeas were grown at two different planting densities. Pinto bean production was 341.5 g dry seed m -2 (5.42 g m -2 day -1) and 579.5 dry seed m -2 (9.20 g m -2 day -1) at planted densities of 32.5 plants m -2 and 37.5 plants m -2, respectively. Cowpea yielded 187.9 g dry seed m -2 (2.21 g m -2 day -1) and 348.8 dry seed m -2 (4.10 g m -2 day -1) at planted densities of 20.8 plants m -2 and 27.7 plants m -2, respectively. The crop was grown at elevated atmospheric carbon dioxide levels, with levels ranging from 300-3000 ppm daily during the majority of the crop cycle. During early stages (first 10 days) of the crop, CO 2 was allowed to rise to 7860 ppm while soil respiration dominated, and then was brought down by plant photosynthesis. CO 2 was injected 27 times during days 29-71 to replenish CO 2 used by the crop during photosynthesis. Temperature regime was 24-28 °C day/deg 20-24 °C night. Pinto bean matured and was harvested 20 days earlier than is typical for this variety, while the cowpea, which had trouble establishing, took 25 days more for harvest than typical for this variety. Productivity and atmospheric dynamic results of these studies contribute toward the design of an envisioned ground-based test bed prototype Mars base.

Lateral Mixing DRI Analysis: Submesoscale Water-Mass Spectra

DTIC Science & Technology

2013-09-30

program to determine submesoscale variability in the Sargasso Sea under weak-to-moderate mesoscale conditions. Two sites were examined, a quiet site...anomalies and dye streaks. Hammerhead carries finescale Sea -Bird sensors for temperature, conductivity and pressure as well as Chelsea and WetLab...m of dye-injection target densities. They were embedded in 35-km towyo grid surveys by Craig Lee’s Triaxus and 15-km butterfly surveys by Jody

Criminal Investigation Program Report

DTIC Science & Technology

1988-10-31

Periodontal Resident for his paper "The Presence of Nicotine on Root Surfaces of Periodontally Diseased Teeth in Smokers" The paper based on Protocol #87-20...Closed Root 87-23 Planing with a Conventional Approach. (C) 31 1987 Evaluation of the Apical Seal Produced by Injected 87-32 Thermoplasticized Gutta...Freeze-Dried Bone. (0) 35 1988 Evaluation of Osseointegration of Titanium Dental 88-11 Implants in Micro Swine. (0) 36 1988 Experimental Periodontitis

A Cadaveric Study on Sacroiliac Joint Injection

PubMed Central

Zou, Yu-Cong; Li, Yi-Kai; Yu, Cheng-Fu; Yang, Xian-Wen; Chen, Run-Qi

2015-01-01

The scope of this study was to explore the possibility as well as the feasibility of sacroiliac joint injection following simple X-ray clip location. For the cadaveric study, 10 fixed sacroiliac joint (SIJ) sectional specimens, 4 dried cadaveric pelvises and 21 embalmed adult cadaveric pelvises were dissected, followed by an injection of contrast agent into the joint. The irrigation of the agent was observed through CT scanning. For the radiologic study, 188 CT scans of ankylosing spondylitis patients (143 male, 45 female) were collected from 2010 to 2012, in Nanfang Hospital. What was measured was (1) Distance between the posterior midline and sagittal synovium; (2) Length of the sagittal synovium; (3) Distance between the midpoint of the sagittal synovium and posterior superior iliac spine; and (4) Distance between the superficial skin vertical to the sagittal synovium point were measured. For the practice-based study: 20 patients (17 males and 3 females) with early ankylosing spondylitis, from Nanfang Hospital affiliated with Southern Medical University were recruited, and sacroiliac joint unguided injections were done on the basis of the cadaveric and radiologic study. Only the inferior 1/3rd portion parallel to the posterior midline could be injected into since the superior 2/3rd portion were filled with interosseous ligaments. Thirteen of the 20 patients received successful injections as identified by CT scan using the contrast agent. Sacroiliac joint injection following simple X-ray clip location is possible and feasible if the operation is performed by trained physicians familiar with the sacroiliac joint and its surrounding anatomic structures. PMID:25692437

Development of integrated control system for smart factory in the injection molding process

NASA Astrophysics Data System (ADS)

Chung, M. J.; Kim, C. Y.

2018-03-01

In this study, we proposed integrated control system for automation of injection molding process required for construction of smart factory. The injection molding process consists of heating, tool close, injection, cooling, tool open, and take-out. Take-out robot controller, image processing module, and process data acquisition interface module are developed and assembled to integrated control system. By adoption of integrated control system, the injection molding process can be simplified and the cost for construction of smart factory can be inexpensive.

Comparative evaluation of serum, FTA filter-dried blood and oral fluid as sample material for PRRSV diagnostics by RT-qPCR in a small-scale experimental study.

PubMed

Steinrigl, Adolf; Revilla-Fernández, Sandra; Wodak, Eveline; Schmoll, Friedrich; Sattler, Tatjana

2014-01-01

Recently, research into alternative sample materials, such as oral fluid or filter-dried blood has been intensified, in order to facilitate cost-effective and animal-friendly sampling of individuals or groups of pigs for diagnostic purposes. The objective of this study was to compare the sensitivity of porcine reproductive and respiratory syndrome virus (PRRSV)-RNA detection by reverse transcription quantitative real-time PCR (RT-qPCR) in serum, FTA filter-dried blood and oral fluid sampled from individual pigs. Ten PRRSV negative pigs were injected with an EU-type PRRSV live vaccine. Blood and oral fluid samples were taken from each pig before, and 4, 7, 14 and 21 days after vaccination. All samples were then analyzed by PRRSV RT-qPCR. In serum, eight often pigs tested RT-qPCR positive at different time points post infection. Absolute quantification showed low serum PRRSV-RNA loads in most samples. In comparison to serum, sensitivity of PRRSV-RNA detection was strongly reduced in matched FTA filter-dried blood and in oral fluid from the same pigs. These results indicate that with low PRRSV-RNA loads the diagnostic sensitivity of PRRSV-RNA detection by RT-qPCR achieved with serum is currently unmatched by either FTA filter-dried blood or oral fluid.

A pilot study using a novel pyrotechnically driven prototype applicator for epidermal powder immunization in piglets.

PubMed

Engert, Julia; Anamur, Cihad; Engelke, Laura; Fellner, Christian; Lell, Peter; Henke, Stefan; Stadler, Julia; Zöls, Susanne; Ritzmann, Mathias; Winter, Gerhard

2018-04-20

Epidermal powder immunization (EPI) is an alternative technique to the classical immunization route using needle and syringe. In this work, we present the results of an in vivo pilot study in piglets using a dried influenza model vaccine which was applied by EPI using a novel pyrotechnically driven applicator. A liquid influenza vaccine (Pandemrix ® ) was first concentrated by tangential flow filtration and hemagglutinin content was determined by RP-HPLC. The liquid formulation was then transformed into a dry powder by collapse freeze-drying and subsequent cryo-milling. The vaccine powder was attached to a membrane of a novel pyrotechnical applicator using oily adjuvant components. Upon actuation of the applicator, particles were accelerated to high speed as determined by a high-speed camera setup. Piglets were immunized twice using either the novel pyrotechnical applicator or classical intramuscular injection. Blood samples of the animals were collected at various time points and analyzed by enzyme-linked immunosorbent assay. Our pilot study shows that acceleration of a dried vaccine powder to supersonic speed using the pyrotechnical applicator is possible and that the speed and impact of the particles is sufficient to breach the stratum corneum of piglet skin. Importantly, the administration of the dry vaccine powder resulted in measurable anti-H1N1 antibody titres in vivo. Copyright © 2018 Elsevier B.V. All rights reserved.

INVESTIGATION OF DRY-WEATHER POLLUTANT ENTRIES INTO STORM-DRAINAGE SYSTEMS

EPA Science Inventory

This article describes the results of a series of research tasks to develop a procedure to investigate non-stormwater (dry-weather) entries into storm drainage systems. Dry-weather flows discharging from storm drainage systems can contribute significant pollutant loadings to rece...

Hydraulic fracturing in granite under geothermal conditions

USGS Publications Warehouse

Solberg, P.; Lockner, D.; Byerlee, J.D.

1980-01-01

The experimental hydraulic fracturing of granite under geothermal conditions produces tensile fracture at rapid fluid injection rates and shear fracture at slow injection rates and elevated differential stress levels. A sudden burst of acoustic emission activity accompanies tensile fracture formation whereas the acoustic emission rate increases exponentially prior to shear fracture. Temperature does not significantly affect the failure mechanism, and the experimental results have not demonstrated the occurrence of thermal fracturing. A critical result of these experiments is that fluid injection at intermediate rates and elevated differential stress levels increases permeability by more than an order of magnitude without producing macroscopic fractures, and low-level acoustic emission activity occurs simultaneously near the borehole and propagates outward into the specimen with time. Permeability measurements conducted at atmospheric pressure both before and after these experiments show that increased permeability is produced by permanent structural changes in the rock. Although results of this study have not demonstrated the occurrence of thermal fracturing, they suggest that fluid injection at certain rates in situ may markedly increase local permeability. This could prove critical to increasing the efficiency of heat exchange for geothermal energy extraction from hot dry rock. ?? 1980.

Ovarian response to pregnant kare serum gonadotrophin and prostaglandin F(2) proportional, variant in Africander and Mashona cows.

PubMed

Holness, D H; Hale, D H; McCabe, C T

1980-11-01

Oestrus was synchronised in ten Africander and eight Mashona mature dry cows by two injections of prostaglandin F(2) proportional, variant (PG) 11 days apart. Half the cows of each breed received an injection of 3000 i.u. pregnant mare serum gonadotrophin (PMSG) two days prior to the second PG injection. All cows were observed for the incidence of cestrus, and blood samples were taken at intervals for progesterone assay. Cows were slaughtered 11 days after the second PG injection and their reproductive tracts examined. Treatment with PMSG increased numbers both of corpora lutea and of follicles more than 10 mm in diameter. When numbers of corpora lutea and follicles were considered together, the response to treatment was significant in the Africanders (P<0,01) and markedly greater than that of Kashona cows. The concentration of progesterone in plasma on the day before slaughter was significantly correlated with the mass of corpora lutea (P<0,001), total mass of ovaries (P<0,001), but not with numbers of corpora lutea. It is suggested that generally Africander cows may secrete lower levels of follicle stimulating hormone and oestrogen than kashona cows during normal cyclic sexual activity.

A Method of Lyophilizing Vaccines Containing Aluminum Salts into a Dry Powder Without Causing Particle Aggregation or Decreasing the Immunogenicity Following Reconstitution

PubMed Central

Li, Xinran; Thakkar, Sachin G.; Ruwona, Tinashe B.; Williams, Robert O.; Cui, Zhengrong

2015-01-01

Many currently licensed and commercially available human vaccines contain aluminum salts as vaccine adjuvants. A major limitation with these vaccines is that they must not be exposed to freezing temperatures during transport or storage such that the liquid vaccine freezes, because freezing causes irreversible coagulation that damages the vaccines (e.g., loss of efficacy). Therefore, vaccines that contain aluminum salts as adjuvants are formulated as liquid suspensions and are required to be kept in cold chain (2–8°C) during transport and storage. Formulating vaccines adjuvanted with aluminum salts into dry powder that can be readily reconstituted before injection may address the limitation. Spray freeze-drying of vaccines with low concentrations of aluminum salts and high concentrations of trehalose alone, or a mixture of sugars and amino acids, as excipients can convert vaccines containing aluminum salts into dry powder, but fails to preserve the particle size and/or immunogenicity of the vaccines. In the present study, using ovalbumin as a model antigen adsorbed onto aluminum hydroxide or aluminum phosphate, a commercially available tetanus toxoid vaccine adjuvanted with potassium alum, a human hepatitis B vaccine adjuvanted with aluminum hydroxide, and a human papillomavirus vaccine adjuvanted with aluminum hydroxyphosphate sulfate, it was shown that vaccines containing a relatively high concentration of aluminum salts (i.e., up to ~1%, w/v, of aluminum hydroxide) can be converted into a dry powder by thin-film freezing followed by removal of the frozen solvent by lyophilization while using low levels of trehalose (i.e., as low as 2% w/v) as an excipient. Importantly, the thin-film freeze-drying process did not cause particle aggregation, nor decreased the immunogenicity of the vaccines. Moreover, repeated freezing-and-thawing of the dry vaccine powder did not cause aggregation. Thin-film freeze-drying is a viable platform technology to produce dry powders of vaccines that contain aluminum salts. PMID:25735896

Emulation study on system characteristic of high pressure common-rail fuel injection system for marine medium-speed diesel engine

NASA Astrophysics Data System (ADS)

Wang, Qinpeng; Yang, Jianguo; Xin, Dong; He, Yuhai; Yu, Yonghua

2018-05-01

In this paper, based on the characteristic analyzing of the mechanical fuel injection system for the marine medium-speed diesel engine, a sectional high-pressure common rail fuel injection system is designed, rated condition rail pressure of which is 160MPa. The system simulation model is built and the performance of the high pressure common rail fuel injection system is analyzed, research results provide the technical foundation for the system engineering development.

Drying Characteristics and Water-soluble Polysaccharides Evaluation of Kidney Shape Ganoderma lucidum Drying in Air Circulation System

NASA Astrophysics Data System (ADS)

Prasetyo, D. J.; Jatmiko, T. H.; Poeloengasih, C. D.; Kismurtono, M.

2017-12-01

In this project, drying kinetic of kidney shape Ganoderma lucidum fruiting body in air circulation system was studied. The drying experiments were conducted at 40, 50 and 60°C with air flow rate of 1.3 ms-1. Samples were weighted periodically until no change in sample weight was recorded, and then the samples were analyzed for its moisture content. Four different thin-layer mathematical models (Newton, Page, Two-term, Midilli) were used and compare to evaluate the drying curves of kidney shape G. lucidum. The water-soluble polysaccharides were evaluated in order to find the best drying temperature condition. The results indicates that Midilli model was the fittest model to describe the characteristic of kidney shape G. lucidum in the air circulation drying system and temperature of 50°C was the best drying condition to get highest value of water-soluble polysaccharides.

Development of infrared thermal imager for dry eye diagnosis

NASA Astrophysics Data System (ADS)

Chiang, Huihua Kenny; Chen, Chih Yen; Cheng, Hung You; Chen, Ko-Hua; Chang, David O.

2006-08-01

This study aims at the development of non-contact dry eye diagnosis based on an infrared thermal imager system, which was used to measure the cooling of the ocular surface temperature of normal and dry eye patients. A total of 108 subjects were measured, including 26 normal and 82 dry eye patients. We have observed that the dry eye patients have a fast cooling of the ocular surface temperature than the normal control group. We have developed a simplified algorithm for calculating the temperature decay constant of the ocular surface for discriminating between normal and dry eye. This study shows the diagnostic of dry eye syndrome by the infrared thermal imager system has reached a sensitivity of 79.3%, a specificity of 75%, and the area under the ROC curve 0.841. The infrared thermal imager system has a great potential to be developed for dry eye screening with the advantages of non-contact, fast, and convenient implementation.

Ultrasound-guided interventional procedures around the shoulder.

PubMed

Messina, Carmelo; Banfi, Giuseppe; Orlandi, Davide; Lacelli, Francesca; Serafini, Giovanni; Mauri, Giovanni; Secchi, Francesco; Silvestri, Enzo; Sconfienza, Luca Maria

2016-01-01

Ultrasound is an established modality for shoulder evaluation, being accurate, low cost and radiation free. Different pathological conditions can be diagnosed using ultrasound and can be treated using ultrasound guidance, such as degenerative, traumatic or inflammatory diseases. Subacromial-subdeltoid bursitis is the most common finding on ultrasound evaluation for painful shoulder. Therapeutic injections of corticosteroids are helpful to reduce inflammation and pain. Calcific tendinopathy of rotator cuff affects up to 20% of painful shoulders. Ultrasound-guided treatment may be performed with both single- and double-needle approach. Calcific enthesopathy, a peculiar form of degenerative tendinopathy, is a common and mostly asymptomatic ultrasound finding; dry needling has been proposed in symptomatic patients. An alternative is represented by autologous platelet-rich plasma injections. Intra-articular injections of the shoulder can be performed in the treatment of a variety of inflammatory and degenerative diseases with corticosteroids or hyaluronic acid respectively. Steroid injections around the long head of the biceps brachii tendon are indicated in patients with biceps tendinopathy, reducing pain and humeral tenderness. The most common indication for acromion-clavicular joint injection is degenerative osteoarthritis, with ultrasound representing a useful tool in localizing the joint space and properly injecting various types of drugs (steroids, lidocaine or hyaluronic acid). Suprascapular nerve block is an approved treatment for chronic shoulder pain non-responsive to conventional treatments as well as candidate patients for shoulder arthroscopy. This review provides an overview of these different ultrasonography-guided procedures that can be performed around the shoulder.

Two-step rapid sulfur capture. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1994-04-01

The primary goal of this program was to test the technical and economic feasibility of a novel dry sorbent injection process called the Two-Step Rapid Sulfur Capture process for several advanced coal utilization systems. The Two-Step Rapid Sulfur Capture process consists of limestone activation in a high temperature auxiliary burner for short times followed by sorbent quenching in a lower temperature sulfur containing coal combustion gas. The Two-Step Rapid Sulfur Capture process is based on the Non-Equilibrium Sulfur Capture process developed by the Energy Technology Office of Textron Defense Systems (ETO/TDS). Based on the Non-Equilibrium Sulfur Capture studies the rangemore » of conditions for optimum sorbent activation were thought to be: activation temperature > 2,200 K for activation times in the range of 10--30 ms. Therefore, the aim of the Two-Step process is to create a very active sorbent (under conditions similar to the bomb reactor) and complete the sulfur reaction under thermodynamically favorable conditions. A flow facility was designed and assembled to simulate the temperature, time, stoichiometry, and sulfur gas concentration prevalent in the advanced coal utilization systems such as gasifiers, fluidized bed combustors, mixed-metal oxide desulfurization systems, diesel engines, and gas turbines.« less

Air Sample Conditioner Helps the Waste Treatment Plant Meet Emissions Standards

DOE Office of Scientific and Technical Information (OSTI.GOV)

Glissmeyer, John A.; Flaherty, Julia E.; Pekour, Mikhail S.

2014-12-02

The air in three of the Hanford Site Waste Treatment and Immobilization Plant (WTP) melter off-gas discharge stacks will be hot and humid after passing through the train of emission abatement equipment. The off-gas temperature and humidity levels will be incompatible with the airborne emissions monitoring equipment required for this type of stack. To facilitate sampling from these facilities, an air sample conditioner system will be installed to introduce cool, dry air into the sample stream to reduce the temperature and dew point. This will avoid thermal damage to the instrumentation and problematic condensation. The complete sample transport system mustmore » also deliver at least 50% of the particles in the sample airstream to the sample collection and on-line analysis equipment. The primary components of the sample conditioning system were tested in a laboratory setting. The sample conditioner itself is based on a commercially-available porous tube filter design. It consists of a porous sintered metal tube inside a coaxial metal jacket. The hot gas sample stream passes axially through the porous tube, and the dry, cool air is injected into the jacket and through the porous wall of the inner tube, creating an effective sample diluter. The dilution and sample air mix along the entire length of the porous tube, thereby simultaneously reducing the dew point and temperature of the mixed sample stream. Furthermore, because the dilution air enters through the porous tube wall, the sample stream does not come in contact with the porous wall and particle deposition is reduced in this part of the sampling system. Tests were performed with an environmental chamber to supply air with the temperature and humidity needed to simulate the off-gas conditions. Air from the chamber was passed through the conditioning system to test its ability to reduce the temperature and dew point of the sample stream. To measure particle deposition, oil droplets in the range of 9 to 11 micrometer aerodynamic diameter were injected into the environmental chamber and drawn through the conditioning system, which included a filter to capture droplets that passed through the conditioner. The droplets were tagged with a fluorescent dye which allowed quantification of droplet deposition on each component of the system. The tests demonstrated the required reductions in temperature and moisture, with no condensation forming when heat tracing was added on the upstream end of the sample conditioner. Additionally, tests indicated that the system, operating at several flow rates and in both vertical and horizontal orientations, delivers nearly all of the sampled particles for analysis. Typical aerosol penetration values were between 98 and 99%. PNNL, Bechtel National Inc., and the instrument vendor are working to implement the sample conditioner into the air monitoring systems used for the melter off-gas exhaust streams. Similar technology may be useful for processes in other facilities with air exhaust streams with elevated temperature and/or humidity.« less

Space Chambers for Crop Treatment

NASA Technical Reports Server (NTRS)

1985-01-01

Vacuum chambers, operated by McDonnell Douglas Corporation to test spacecraft, can also be used to dry water-soaked records. The drying temperature is low enough to allow paper to dry without curling or charging. Agricultural crops may also be dried using a spinoff system called MIVAC, which has proven effective in drying rice, wheat, soybeans, corn, etc. The system is energy efficient and can incorporate a sanitation process for destroying insects without contamination.

Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines

PubMed Central

Gregory, James A.; Topol, Aaron B.; Doerner, David Z.

2013-01-01

Infectious diseases disproportionately affect indigent regions and are the greatest cause of childhood mortality in developing countries. Practical, low-cost vaccines for use in these countries are paramount to reducing disease burdens and concomitant poverty. Algae are a promising low-cost system for producing vaccines that can be orally delivered, thereby avoiding expensive purification and injectable delivery. We engineered the chloroplast of the eukaryotic alga Chlamydomonas reinhardtii to produce a chimeric protein consisting of the 25-kDa Plasmodium falciparum surface protein (Pfs25) fused to the β subunit of the cholera toxin (CtxB) to investigate an alga-based whole-cell oral vaccine. Pfs25 is a promising malaria transmission-blocking vaccine candidate that has been difficult to produce in traditional recombinant systems due to its structurally complex tandem repeats of epidermal growth factor-like domains. The noncatalytic CtxB domain of the cholera holotoxin assembles into a pentameric structure and acts as a mucosal adjuvant by binding GM1 ganglioside receptors on gut epithelial cells. We demonstrate that CtxB-Pfs25 accumulates as a soluble, properly folded and functional protein within algal chloroplasts, and it is stable in freeze-dried alga cells at ambient temperatures. In mice, oral vaccination using freeze-dried algae that produce CtxB-Pfs25 elicited CtxB-specific serum IgG antibodies and both CtxB- and Pfs25-specific secretory IgA antibodies. These data suggest that algae are a promising system for production and oral delivery of vaccine antigens, but as an orally delivered adjuvant, CtxB is best suited for eliciting secretory IgA antibodies for vaccine antigens against pathogens that invade mucosal surfaces using this strategy. PMID:23603678

Subconjunctival dendrimer-drug therapy for the treatment of dry eye in rabbits with induced autoimmune dacryoadenitis.

PubMed

Lin, Hui; Liu, Ying; Kambhampati, Siva P; Hsu, Chih-Chien; Kannan, Rangaramanujam M; Yiu, Samuel C

2018-05-16

To investigate the efficacy of a single subconjunctival injection of dendrimer-dexamethasone to treat dry eye in a rabbit model of induced autoimmune dacryoadenitis (AID). Dendrimer biodistribution after subconjuntival injection in AID animals was evaluated using Cy5-labelled dendrimer (D-Cy5) and confocal microscopy. Diseased animals were treated with free dexamethasone (Free-Dex), dendrimer-dexamethasone (D-Dex), or saline via a single subconjunctival injection. The efficacy was evaluated using various clinical evaluations, such as Schirmer's test, tear breakup time (TBUT), and fluorescein and rose bengal staining. Histopathology was evaluated by H&E staining and immunostaining. Levels of inflammatory cytokines and aquaporin proteins in the LGs were determined by real-time PCR. Subconjunctivally administered dendrimers selectively localized in the inflamed LGs, and were taken up by the infiltrated cells. At two weeks post-treatment, the D-Dex group showed improved clinical evaluations. No significant changes were observed in other groups. H&E staining demonstrated, less inflammatory cell infiltration and fewer atrophic acini in D-Dex group, compared to those treated with saline or Free-Dex. Immunohistochemistry demonstrated that the intensity of CD-18 (+) and RTLA (+) was weaker in LGs in the D-Dex group than in other treatment rabbits. Pro-inflammatory gene expression levels of MMP9, IL6, IL8, and TNFα were significantly decreased in the D-Dex group compared to the saline group. The dendrimer exhibits pathology-dependent biodistribution in the inflamed LGs. Subconjunctivally administered D-Dex suppressed LG inflammation, leading to partial recovery of LG function with clinical improvement in induced AID. Sjögren's patients may benefit from this targeted nanomedicine approach. Copyright © 2018. Published by Elsevier Inc.

Determination of Efavirenz in Human Dried Blood Spots by Reversed-Phase High Performance Liquid Chromatography with UV Detection

PubMed Central

Hoffman, Justin T; Rossi, Steven S; Espina-Quinto, Rowena; Letendre, Scott; Capparelli, Edmund V

2013-01-01

Background Previously published methods for determination of efavirenz (EFV) in human dried blood spots (DBS) employ costly and complex liquid chromatography/mass spectrometry. We describe the validation and evaluation of a simple and inexpensive high-performance liquid chromatography (HPLC) method for EFV quantification in human DBS and dried plasma spots (DPS), using ultraviolet (UV) detection appropriate for resource-limited settings. Methods 100μl of heparinized whole blood or plasma were spotted onto blood collection cards, dried, punched, and eluted. Eluates are injected onto a C-18 reversed phase HPLC column. EFV is separated isocratically using a potassium phosphate and ACN mobile phase. UV detection is at 245nm. Quantitation is by use of external calibration standards. Following validation, the method was evaluated using whole blood and plasma from HIV-positive patients undergoing EFV therapy. Results Mean recovery of drug from dried blood spots is 91.5%. The method is linear over the validated concentration range of 0.3125 – 20.0μg/mL. A good correlation (Spearman r=0.96) between paired plasma and DBS EFV concentrations from the clinical samples was observed, and hematocrit level was not found to be a significant determinant of the EFV DBS level. The mean observed CDBS/Cplasma ratio was 0.68. A good correlation (Spearman r=0.96) between paired plasma and DPS EFV concentrations from the clinical samples was observed. The mean percent deviation of DPS samples from plasma samples is 1.68%. Conclusions Dried whole blood spot or dried plasma spot sampling is well suited for monitoring EFV therapy in resource limited settings, particularly when high sensitivity is not essential. PMID:23503446

Use of a double condenser in a dehumidifier with a spray dryer for vitamin A extraction in tomato as a heat-sensitive material

NASA Astrophysics Data System (ADS)

Kosasih, E. A.; Warjito, H., Imansyah I.; Ruhyat, N.

2017-06-01

Spray dryers are commonly operated at a high temperature (>100 °C), which becomes an obstacle for heat-sensitive materials. In this study, a refrigeration system that uses evaporator as dehumidifier and that recovers the heat released from the first condenser to preheat the drying air was utilised to reduce the drying temperature. Results showed that the degradation of vitamin A (measured with the high performance liquid chromatography method) in tomato increased significantly when the drying air temperature increased from 90 °C to 120 °C, and it cannot be controlled at a temperature higher than 120 °C. At an air flow rate of 450 lpm, the drying capacity at a drying air temperature of 60 °C (with refrigeration, humidity ratio of 0.005 [kg H2O / kg dry air]) is equal to the drying capacity at a drying air temperature of 120 °C (without refrigeration, humidity ratio of 0.021 [kg H2O / kg dry air]). The drying capacity at a drying air temperature of 90 °C (with refrigeration) even becomes 1.5 times the drying capacity at a drying air temperature of 120 °C (without refrigeration). The combination of a spray dryer system with a refrigeration system (double condenser) is therefore beneficial for drying heat-sensitive materials, such as vitamin A.

Physicochemical and in vitro deposition properties of salbutamol sulphate/ipratropium bromide and salbutamol sulphate/excipient spray dried mixtures for use in dry powder inhalers.

PubMed

Corrigan, Deirdre O; Corrigan, Owen I; Healy, Anne Marie

2006-09-28

The physicochemical and aerodynamic properties of spray dried powders of the drug/drug mixture salbutamol sulphate/ipratropium bromide were investigated. The in vitro deposition properties of spray dried salbutamol sulphate and the spray dried drug/excipient mixtures salbutamol sulphate/lactose and salbutamol sulphate/PEG were also determined. Spray drying ipratropium bromide monohydrate resulted in a crystalline material from both aqueous and ethanolic solution. The product spray dried from aqueous solution consisted mainly of ipratropium bromide anhydrous. There was evidence of the presence of another polymorphic form of ipratropium bromide. When spray dried from ethanolic solution the physicochemical characterisation suggested the presence of an ipratropium bromide solvate with some anhydrous ipratropium bromide. Co-spray drying salbutamol sulphate with ipratropium bromide resulted in amorphous composites, regardless of solvent used. Particles were spherical and of a size suitable for inhalation. Twin impinger studies showed an increase in the fine particle fraction (FPF) of spray dried salbutamol sulphate compared to micronised salbutamol sulphate. Co-spray dried salbutamol sulphate:ipratropium bromide 10:1 and 5:1 systems also showed an increase in FPF compared to micronised salbutamol sulphate. Most co-spray dried salbutamol sulphate/excipient systems investigated demonstrated FPFs greater than that of micronised drug alone. The exceptions to this were systems containing PEG 4000 20% or PEG 20,000 40% both of which had FPFs not significantly different from micronised salbutamol sulphate. These two systems were crystalline unlike most of the other spray dried composites examined which were amorphous in nature.

Drying based on temperature-detection-assisted control in microwave-assisted pulse-spouted vacuum drying.

PubMed

Cao, Xiaohuang; Zhang, Min; Qian, He; Mujumdar, Arun S

2017-06-01

An online temperature-detection-assisted control system of microwave-assisted pulse-spouted vacuum drying was newly developed. By using this system, temperature control can be automatically and continuously adjusted based on the detection of drying temperature and preset temperature. Various strategies for constant temperature control, linear temperature control and three-step temperature control were applied to drying carrot cubes. Drying kinetics and the quality of various temperature-controlled strategies online are evaluated for the new drying technology as well as its suitability as an alternative drying method. Drying time in 70 °C mode 1 had the shortest drying time and lowest energy consumption in all modes. A suitable colour, highest re-hydration ratio and fracture-hardness, and longest drying time occurred in 30-40-50 °C mode 3. The number of hot spots was reduced in 40-50-60 °C mode 3. Acceptable carrot snacks were obtained in 50-60-70 °C mode 3 and 70 °C mode 2. All temperature curves showed that the actual temperatures followed the preset temperatures appropriately. With this system, a linear temperature-controlled strategy and a three-step temperature-controlled strategy can improve product quality and heating non-uniformity compared to constant temperature control, but need greater energy consumption and longer drying time. A temperature-detection-assisted control system was developed for providing various drying strategies as a suitable alternative in making a snack product. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Drying hardwood lumber

Treesearch

Joseph Denig; Eugene M. Wengert; William T. Simpson

2000-01-01

Drying Hardwood Lumber focuses on common methods for drying lumber of different thickness, with minimal drying defects, for high quality applications. This manual also includes predrying treatments that, when part of an overall quality-oriented drying system, reduce defects and improve drying quality, especially of oak lumber. Special attention is given to drying white...

Localization of wood floor structure by infrared thermography

NASA Astrophysics Data System (ADS)

Cochior Plescanu, C.; Klein, M.; Ibarra-Castanedo, C.; Bendada, A.; Maldague, X.

2008-03-01

One of our industrial partners, Assek Technologie, is interested in developing a technique that would improve the drying process of wood floor in basements after flooding. In order to optimize the procedure, the floor structure and the damaged (wet) area extent must first be determined with minimum intrusion (minimum or no dismantling). The present study presents the use of infrared thermography to reveal the structure of (flooded) wood floors. The procedure involves opening holes in the floor. Injecting some hot air through those holes reveals the framing structure even if the floor is covered by vinyl or ceramic tiles. This study indicates that thermal imaging can also be used as a tool to validate the decontamination process after drying. Thermal images were obtained on small-scale models and in a demonstration room.

Electrokinetic injection techniques in microfluidic chips.

PubMed

Fu, L M; Yang, R J; Lee, G B; Liu, H H

2002-10-01

The separation efficiency of a microfluidic chip is influenced to a significant degree by the flow field conditions within the injection microchannel. Therefore, an understanding of the physics of the flow within this channel is beneficial in the design and operation of such a system. The configuration of an injection system is determined by the volume of the sample plug that is to be delivered to the separation process. Accordingly, this paper addresses the design and testing of injection systems with a variety of configurations, including a simple cross, a double-T, and a triple-T configuration. This paper also presents the design of a unique multi-T injection configuration. Each injection system cycles through a predetermined series of steps, in which the electric field magnitude and distribution within the various channels is strictly manipulated, to effectuate a virtual valve. The uniquemulti-T configuration injection system presented within this paper has the ability to simulate the functions of the cross, double-T, and triple-T systems through appropriate manipulations of the electric field within its various channels. In other words, the proposed design successfully combines several conventional injection systems within a single microfluidic chip.

Dry transfer system for spent fuel: Project report, A system designed to achieve the dry transfer of bare spent fuel between two casks. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dawson, D.M.; Guerra, G.; Neider, T.

1995-12-01

This report describes the system developed by EPRI/DOE for the dry transfer of spent fuel assemblies outside the reactor spent fuel pool. The system is designed to allow spent fuel assemblies to be removed from a spent fuel pool in a small cask, transported to the transfer facility, and transferred to a larger cask, either for off-site transportation or on-site storage. With design modifications, this design is capable of transferring single spent fuel assemblies from dry storage casks to transportation casks or visa versa. One incentive for the development of this design is that utilities with limited lifting capacity ormore » other physical or regulatory constraints are limited in their ability to utilize the current, more efficient transportation and storage cask designs. In addition, DOE, in planning to develop and implement the multi-purpose canister (MPC) system for the Civilian Radioactive Waste Management System, included the concept of an on-site dry transfer system to support the implementation of the MPC system at reactors with limitations that preclude the handling of the MPC system transfer casks. This Dry Transfer System can also be used at reactors wi decommissioned spent fuel pools and fuel in dry storage in non-MPC systems to transfer fuel into transportation casks. It can also be used at off-reactor site interim storage facilities for the same purpose.« less

From cellular lysis to microarray detection, an integrated thermoplastic elastomer (TPE) point of care Lab on a Disc.

PubMed

Roy, Emmanuel; Stewart, Gale; Mounier, Maxence; Malic, Lidija; Peytavi, Régis; Clime, Liviu; Madou, Marc; Bossinot, Maurice; Bergeron, Michel G; Veres, Teodor

2015-01-21

We present an all-thermoplastic integrated sample-to-answer centrifugal microfluidic Lab-on-Disc system (LoD) for nucleic acid analysis. The proposed CD system and engineered platform were employed for analysis of Bacillus atrophaeus subsp. globigii spores. The complete assay comprised cellular lysis, polymerase chain reaction (PCR) amplification, amplicon digestion, and microarray hybridization on a plastic support. The fluidic robustness and operating efficiency of the assay were ensured through analytical optimization of microfluidic tools enabling beneficial implementation of capillary valves and accurate control of all flow timing procedures. The assay reliability was further improved through the development of two novel microfluidic strategies for reagents mixing and flow delay on the CD platform. In order to bridge the gap between the proof-of-concept LoD and production prototype demonstration, low-cost thermoplastic elastomer (TPE) was selected as the material for CD fabrication and assembly, allowing the use of both, high quality hot-embossing and injection molding processes. Additionally, the low-temperature and pressure-free assembly and bonding properties of TPE material offer a pertinent solution for simple and efficient loading and storage of reagents and other on-board components. This feature was demonstrated through integration and conditioning of microbeads, magnetic discs, dried DNA buffer reagents and spotted DNA array inserts. Furthermore, all microfluidic functions and plastic parts were designed according to the current injection mold-making knowledge for industrialization purposes. Therefore, the current work highlights a seamless strategy that promotes a feasible path for the transfer from prototype toward realistic industrialization. This work aims to establish the full potential for TPE-based centrifugal system as a mainstream microfluidic diagnostic platform for clinical diagnosis, water and food safety, and other molecular diagnostic applications.

33 CFR 149.416 - What are the requirements for a dry chemical fire suppression system?

Code of Federal Regulations, 2010 CFR

2010-07-01

... the requirements for a dry chemical fire suppression system? Each natural gas deepwater port must be... dry chemical fire suppression system? 149.416 Section 149.416 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DEEPWATER PORTS DEEPWATER PORTS: DESIGN, CONSTRUCTION...

Single column comprehensive analysis of pharmaceutical preparations using dual-injection mixed-mode (ion-exchange and reversed-phase) and hydrophilic interaction liquid chromatography.

PubMed

Kazarian, Artaches A; Taylor, Mark R; Haddad, Paul R; Nesterenko, Pavel N; Paull, Brett

2013-12-01

The comprehensive separation and detection of hydrophobic and hydrophilic active pharmaceutical ingredients (APIs), their counter-ions (organic, inorganic) and excipients, using a single mixed-mode chromatographic column, and a dual injection approach is presented. Using a mixed-mode Thermo Fisher Acclaim Trinity P1 column, APIs, their counter-ions and possible degradants were first separated using a combination of anion-exchange, cation-exchange and hydrophobic interactions, using a mobile phase consisting of a dual organic modifier/salt concentration gradient. A complementary method was also developed using the same column for the separation of hydrophilic bulk excipients, using hydrophilic interaction liquid chromatography (HILIC) under high organic solvent mobile phase conditions. These two methods were then combined within a single gradient run using dual sample injection, with the first injection at the start of the applied gradient (mixed-mode retention of solutes), followed by a second sample injection at the end of the gradient (HILIC retention of solutes). Detection using both ultraviolet absorbance and refractive index enabled the sensitive detection of APIs and UV-absorbing counter-ions, together with quantitative determination of bulk excipients. The developed approach was applied successfully to the analysis of a dry powder inhalers (Flixotide(®), Spiriva(®)), enabling comprehensive quantification of all APIs and excipients in the sample. Copyright © 2013 Elsevier B.V. All rights reserved.

Investigating high-concentration monoclonal antibody powder suspension in nonaqueous suspension vehicles for subcutaneous injection.

PubMed

Bowen, Mayumi; Armstrong, Nick; Maa, Yuh-Fun

2012-12-01

Developing high-concentration monoclonal antibody (mAb) liquid formulations for subcutaneous (s.c.) administration is challenging because increased viscosity makes injection difficult. To overcome this obstacle, we investigated a nonaqueous powder suspension approach. Three IgG1 mAbs were spray dried and suspended at different concentrations in Miglyol® 840, benzyl benzoate, or ethyl lactate. Suspensions were characterized for viscosity, particle size, and syringeability; physical stability was visually inspected. Suspensions generally outperformed liquid solutions for injectability despite higher viscosity at the same mAb concentrations. Powder formulations and properties had little effect on viscosity or injectability. Ethyl lactate suspensions had lowest viscosity (<20 cP) and lowest syringe injection glide force (<15 N) at mAb concentrations as high as 333 mg/mL (500 mg powder/mL). Inverse gas chromatography analysis indicated that the vehicle was the most important factor impacting suspension performance. Ethyl lactate rendered greater heat of sorption (suggesting strong particle-suspension vehicle interaction may reduce particle-particle self-association, leading to low suspension viscosity and glide force) but lacked the physical suspension stability exhibited by the other vehicles. Specific mixtures of ethyl lactate and Miglyol® 840 improved overall performance in high mAb concentration suspensions. This study demonstrated the viability of high mAb concentration (>300 mg/mL) in suspension formulations for s.c. administration. Copyright © 2012 Wiley Periodicals, Inc.

Low-nitrogen oxides combustion of dried sludge using a pilot-scale cyclone combustor with recirculation.

PubMed

Shim, Sung Hoon; Jeong, Sang Hyun; Lee, Sang-Sup

2015-04-01

Recently, numerical and experimental studies have been conducted to develop a moderate or intense low-oxygen dilution (MILD) combustion technology for solid fuels. The study results demonstrated that intense recirculation inside the furnace by high-momentum air is a key parameter to achieve the MILD combustion of solid fuels. However, the high-velocity air requires a significant amount of electricity consumption. A cyclone-type MILD combustor was therefore designed and constructed in the authors' laboratory to improve the recirculation inside the combustor. The laboratory-scale tests yielded promising results for the MILD combustion of dried sewage sludge. To achieve pilot-scale MILD combustion of dried sludge in this study, the effects of geometric parameters such as the venturi tube configuration, the air injection location, and the air nozzle diameter were investigated. With the optimized geometric and operational conditions, the pilot-scale cyclone combustor demonstrated successful MILD combustion of dried sludge at a rate of 75 kg/hr with an excess air ratio of 1.05. A horizontal cyclone combustor with recirculation demonstrated moderate or intense low-oxygen dilution (MILD) combustion of dried sewage sludge at a rate of 75 kg/hr. Optimizing only geometric and operational conditions of the combustor reduced nitrogen oxide (NOx) emissions to less than 75 ppm. Because the operating cost of the MILD combustor is much lower than that of the selective catalytic reduction (SCR) applied to the conventional combustor, MILD combustion technology with the cyclone type furnace is an eligible option for reducing NOx emissions from the combustion of dried sewage sludge.

Hyperthyroidism results in increased glycolytic capacity in the rat heart. A 31P-NMR study.

PubMed

Seymour, A M; Eldar, H; Radda, G K

1990-11-12

We have investigated the metabolic adaptations that occur in the thyroxine-treated rat heart. Rats were made hyperthyroid by daily intra-peritoneal injections of thyroxine (35 micrograms/100 g body weight) over seven days. 31P-NMR investigations of isolated glucose-perfused isometric hearts showed that thyroxine treatment caused an increase in Pi (from 4.9 mumols.(g dry wt.)-1 in control hearts to 11.7 mumols.(g dry wt.)-1 in hyperthyroid hearts), a decrease in phosphocreatine (from 36.5 mumols.(g dry wt.)-1 to 21.8 mumols.(g dry wt.)-1) with no change in ATP or ADP concentrations under the same conditions of cardiac work. The unidirectional exchange flux Pi----ATP was measured by saturation transfer NMR in hyperthyroid rat hearts. This exchange (which has been shown to contain a significant glycolytic component) increased by 2.2-fold in thyroxine-treated hearts in comparison to control hearts (to 3.6 mumols.(g dry wt.)-1.s-1, from 1.6 mumols.(g dry wt.)-1.s-1). In parallel experiments, NMR analysis of extracts from hyperthyroid rat hearts showed significantly elevated levels of glucose 6-phosphate, and fructose 6-phosphate. Measurements of enzyme activities isolated from hyperthyroid and control tissue showed a 40% increase in phosphofructokinase activity. These data together with the increased concentration of Pi show that both glycolytic and glycogenolytic fluxes are increased in the hyperthyroid rat heart. This metabolic adaptation may be necessary to cope with the increased number and activity of Na+/K(+)-ATPase pumps that occur in response to thyroxine treatment.

Bluff Body Fuel Mixer

NASA Technical Reports Server (NTRS)

Cheung, Albert K. (Inventor); Hoke, James B. (Inventor); McKinney, Randal G. (Inventor)

2017-01-01

A combustor is provided. The combustor may include an axial fuel injection system, and a radial fuel injection system aft of the axial fuel injection system. The axial fuel injection system includes a mixer having a bluff body at an exit port of the mixer, and a fuel injector disposed within the mixer. A fuel and air mixer is also provided and comprises an outer housing with an exit port and a bluff body. The bluff body extends across the exit port of the outer housing. A fuel injection system is also provided. The systems comprise a mixer having a bluff body at an exit port of the mixer and a fuel injector disposed within the mixer.

Capsule injection system for a hydraulic capsule pipelining system

DOEpatents

Liu, Henry

1982-01-01

An injection system for injecting capsules into a hydraulic capsule pipelining system, the pipelining system comprising a pipeline adapted for flow of a carrier liquid therethrough, and capsules adapted to be transported through the pipeline by the carrier liquid flowing through the pipeline. The injection system comprises a reservoir of carrier liquid, the pipeline extending within the reservoir and extending downstream out of the reservoir, and a magazine in the reservoir for holding capsules in a series, one above another, for injection into the pipeline in the reservoir. The magazine has a lower end in communication with the pipeline in the reservoir for delivery of capsules from the magazine into the pipeline.

The effect of baicalin in a mouse model of retinopathy of prematurity

PubMed Central

Jo, Hyoung; Jung, Sang Hoon; Yim, Hye Bin; Lee, Sung Jin; Kang, Kui Dong

2015-01-01

Baicalin is a flavonoid derived from the dried root of Scutellaria baicalensis. In this study, oxygen-induced retinopathy was used to characterize the anti-angiogenic properties of baicalin in mice. Pups were exposed to a hyperbaric oxygen environment to induce retinal angiogenesis and were subjected to intraperitoneal injection of baicalin. Avascular area, neovascular tufts, and neovascular lumens were quantified from digital images. Compared to the vehicle, baicalin clearly reduced the central avascular zone and the number of neovascular tufts and lumens. High-dose baicalin (10 mg/kg) significantly reduced the expression of matrix metalloproteinase-2 (MMP-2), MMP-9, angiotensin II, and vascular endothelial growth factor (VEGF). These results show that baicalin is a powerful antiangiogenic compound that attenuates new vessel formation in the retina after systemic administration, and is a candidate substance for therapeutic inhibition of retinal angiogenesis. [BMB Reports 2015; 48(5): 271-276] PMID:25154719

Annular vortex combustor

DOEpatents

Nieh, Sen; Fu, Tim T.

1992-01-01

An apparatus for burning coal water fuel, dry ultrafine coal, pulverized l and other liquid and gaseous fuels including a vertically extending outer wall and an inner, vertically extending cylinder located concentrically within the outer wall, the annnular space between the outer wall and the inner cylinder defining a combustion chamber and the all space within the inner cylinder defining an exhaust chamber. Fuel and atomizing air are injected tangentially near the bottom of the combustion chamber and secondary air is introduced at selected points along the length of the combustion chamber. Combustion occurs along the spiral flow path in the combustion chamber and the combined effects of centrifugal, gravitational and aerodynamic forces cause particles of masses or sizes greater than the threshold to be trapped in a stratified manner until completely burned out. Remaining ash particles are then small enough to be entrained by the flue gas and exit the system via the exhaust chamber in the opposite direction.

Systemic Comorbidities of Dry Eye Syndrome: The Korean National Health and Nutrition Examination Survey V, 2010 to 2012.

PubMed

Roh, Hyun Cheol; Lee, Jimmy K; Kim, Martha; Oh, Jong-Hyun; Chang, Min-Wook; Chuck, Roy S; Park, Choul Yong

2016-02-01

To identify systemic comorbidities in patients with dry eye syndrome in South Korea. From 2010 to 2012, 17,364 participants aged 20 or older were randomly included in the nationwide Korean National Health and Nutrition Examination Survey V. The prevalence of dry eye syndrome and demographics of these patients were investigated. We performed conditional logistic regression analyses based on age, sex, residential area, education level, occupation type, and household income level to obtain the odds ratio for each systemic comorbidity among subjects with and without dry eye syndrome. The prevalence of dry eye syndrome in this study was 10.4%. Age [adjusted odds ratio (AOR): 1.02], female gender (AOR: 3.01), and indoor occupation (AOR: 1.30) were associated with a higher prevalence of dry eye syndrome and found to be less prevalent in those residing in rural areas (AOR: 0.73) and with lower education levels (AOR: 0.66-0.99). With regard to systemic comorbidities, dyslipidemia (AOR: 1.63), degenerative arthritis (AOR: 1.56), rheumatoid arthritis (AOR: 1.44), thyroid disease (AOR: 1.79), and renal failure (AOR: 2.56) were associated with a significantly higher prevalence of dry eye syndrome. We found that patients with dry eye syndrome have a higher prevalence of several systemic comorbidities. A more comprehensive therapeutic approach considering the effect of systemic medication may be necessary in these patients.

Deep lamellar keratoplasty on air with lyophilised tissue.

PubMed Central

Chau, G K; Dilly, S A; Sheard, C E; Rostron, C K

1992-01-01

Deep lamellar keratoplasty on air involves injecting air into the corneal stroma to expand it to several times its normal thickness. This method is designed to facilitate dissection of the deep stroma and reduce the risk of perforation of Descemet's membrane when carrying out deep lamellar keratoplasty. We have modified the technique by using prelathed freeze-dried donor tissue and report our results in a series of patients with corneal stromal scarring owing to a variety of corneal problems, namely, keratoconus, pterygium, and herpes zoster ophthalmicus. All patients achieved best corrected postoperative visual acuity of 6/12 or better without problems associated with graft failure or rejection. Histopathological examination of corneal tissue following air injection showed surgical emphysema within the cornea and separation of deep stromal fibres from the underlying Descemet's membrane. Images PMID:1477037

CO2 (dry ice) cleaning system

NASA Technical Reports Server (NTRS)

Barnett, Donald M.

1995-01-01

Tomco Equipment Company has participated in the dry ice (solid carbon dioxide, CO2) cleaning industry for over ten years as a pioneer in the manufacturer of high density, dry ice cleaning pellet production equipment. For over four years Tomco high density pelletizers have been available to the dry ice cleaning industry. Approximately one year ago Tomco introduced the DI-250, a new dry ice blast unit making Tomco a single source supplier for sublimable media, particle blast, cleaning systems. This new blast unit is an all pneumatic, single discharge hose device. It meters the insertion of 1/8 inch diameter (or smaller), high density, dry ice pellets into a high pressure, propellant gas stream. The dry ice and propellant streams are controlled and mixed from the blast cabinet. From there the mixture is transported to the nozzle where the pellets are accelerated to an appropriate blasting velocity. When directed to impact upon a target area, these dry ice pellets have sufficient energy to effectively remove most surface coatings through dry, abrasive contact. The meta-stable, dry ice pellets used for CO2 cleaning, while labeled 'high density,' are less dense than alternate, abrasive, particle blast media. In addition, after contacting the target surface, they return to their equilibrium condition: a superheated gas state. Most currently used grit blasting media are silicon dioxide based, which possess a sharp tetrahedral molecular structure. Silicon dioxide crystal structures will always produce smaller sharp-edged replicas of the original crystal upon fracture. Larger, softer dry ice pellets do not share the same sharp-edged crystalline structures as their non-sublimable counterparts when broken. In fact, upon contact with the target surface, dry ice pellets will plastically deform and break apart. As such, dry ice cleaning is less harmful to sensitive substrates, workers and the environment than chemical or abrasive cleaning systems. Dry ice cleaning system components include: a dry ice pellet supply, a non-reactive propellant gas source, a pellet and propellant metering device, and a media transport and acceleration hose and nozzle arrangement. Dry ice cleaning system operating parameters include: choice of propellant gas, its pressure and temperature, dry ice mass flow rate, dry ice pellet size and shape, and acceleration nozzle configuration. These parameters may be modified to fit different applications. The growth of the dry ice cleaning industry will depend upon timely data acquisition of the effects that independent changes in these parameters have on cleaning rates, with respect to different surface coating and substrate combinations. With this data, optimization of cleaning rates for particular applications will be possible. The analysis of the applicable range of modulation of these parameters, within system component mechanical constraints, has just begun.

[Controversy: botulinum toxin, does it prevent cutaneous aging?].

PubMed

Cohen-Letessier, A

2009-05-01

By blocking muscular contraction, even if this effect is reversible, botulinum toxin injected early and regularly to prevent aging modifies the facial traits and can result in muscular and cutaneous atrophy. Mechanical information is indispensable to cell survival and conditions the function of fibroblastic synthesis. Aging cannot be reduced to the facial lines; botulinum toxin cannot prevent the other parameters of age such as drying, pigmentation problems, vascular problems, and loss of volume.

AUTOMATIC RECORDING OF THE RADIOACTIVITY OF ZONES ELUTED FROM THE GAS- LIQUID CHROMATOGRAM

DOE Office of Scientific and Technical Information (OSTI.GOV)

James, A.T.; Piper, E.A.

1961-03-01

A simple proportional flow counter for use with the gas chromatogram is described. which is operated at room temperature by burning all eluted material to CO/sub 2/ over heated copper oxide. The gas stream is dried. 5% CO/sub 2/ is injected and the mixture passed into the counter. Details are given of the necessary circuitry. Examples are provided of its use with long chain fatty acids. (auth)

Unclassified Publications of Lincoln Laboratory, 1 January-31 December 1987. Volume 13

DTIC Science & Technology

1987-12-31

Visible-Laser Photochemical Etching of Cr , Mo, and W 5901 High-Speed Electronic Beam Steering Using Injection Locking of a Laser-Diode Array...of High- Power Broad-Area Diode Lasers High-Temperature Point-Contact Transistors and Schottky Diodes Formed on Synthetic Boron- Doped Diamond...SPEECHES MS No. 593IB C02 Laser Radar 6550B Recent Advances in Transition-Metal- Doped Lasers 6714D Radiation Damage in Dry

A comparative study of spray-dried and freeze-dried hydrocortisone/polyvinyl pyrrolidone solid dispersions.

PubMed

Dontireddy, Rakesh; Crean, Abina M

2011-10-01

Poor water solubility of new chemical entities (NCEs) is one of the major challenges the pharmaceutical industry currently faces. The purpose of this study was to investigate the feasibility of freeze-drying as an alternative technique to spray-drying to produce solid dispersions of poorly water-soluble drugs. Also investigated was the use of aqueous solvent mixtures in place of pure solvent for the production of solid dispersions. Aqueous solvent systems would reduce the environmental impact of pure organic solvent systems. Spray-dried and freeze-dried hydrocortisone/polyvinyl pyrrolidone solid dispersions exhibited differences in dissolution behavior. Freeze-dried dispersions exhibited faster dissolution rates than the corresponding spray-dried dispersions. Spray-dried systems prepared using both solvent systems (20% v/v and 96% v/v ethanol) displayed similar dissolution performance despite displaying differences in glass transition temperatures (T(g)) and surface areas. All dispersions showed drug/polymer interactions indicated by positive deviations in T(g) from the predicted values calculated using the Couchman-Karasz equation. Fourier transform infrared (FTIR) spectroscopic results confirmed the conversion of crystalline drug to the amorphous in the dispersions. Stability studies were preformed at 40°C and 75% relative humidity to investigate the physical stability of prepared dispersions. Recrystallization was observed after a month and the resultant dispersions were tested for their dissolution performance to compare with the dissolution performance of the dispersions prior to the stability study. The dissolution rate of the freeze-dried dispersions remained higher than both spray-dried dispersions after storage.

Control Systems of Rubber Dryer Machinery Components Using Programmable Logic Control (PLC)

NASA Astrophysics Data System (ADS)

Hendra; Yulianto, A. S.; Indriani, A.; Hernadewita; Hermiyetti

2018-02-01

Application of programmable logic control (PLC) is widely used on the control systems in the many field engineering such as automotive, aviation, food processing and other industries [1-2]. PLC is simply program to control many automatic activity, easy to use, flexible and others. PLC using the ladder program to solve and regulated the control system component. In previous research, PLC was used for control system of rotary dryer machine. In this paper PLC are used for control system of motion component in the rubber dryer machinery. Component of rubber dryer machine is motors, gearbox, sprocket, heater, drying chamber and bearing. Principle working of rubber dryer machinery is wet rubber moving into the drying chamber by sprocket. Sprocket is driven by motors that conducted by PLC to moving and set of wet rubber on the drying chamber. Drying system uses greenhouse effect by making hanger dryer design in the form of line path. In this paper focused on motion control system motors and sensors drying rubber using PLC. The results show that control system of rubber dryer machinery can work in accordance control input and the time required to dry the rubber.

New Processes for Freeze-Drying in Dual-Chamber Systems.

PubMed

Werk, T; Ludwig, I S; Luemkemann, J; Huwyler, J; Mahler, H-C; Haeuser, C R; Hafner, M

2016-01-01

Dual-chamber systems can offer self-administration and home care use for lyophilized biologics. Only a few products have been launched in dual-chamber systems so far-presumably due to dual-chamber systems' complex and costly drug product manufacturing process. Within this paper, two improved processes (both based on tray filling technology) for freeze-drying pharmaceuticals in dual-chamber systems are described. Challenges with regards to heat transfer were tackled by (1) performing the freeze-drying step in a needle-down orientation in combination with an aluminum block, or (2) freeze-drying the drug product "externally" in a metal cartridge with subsequent filling of the lyophilized cake into the dual-chamber system. Metal-mediated heat transfer was shown to be efficient in both cases and batch (unit-to-unit) homogeneity with regards to sublimation rate was increased. It was difficult to influence ice crystal size using different methods when in use with an aluminum block due to its heat capacity. Using such a metal carrier implies a large heat capacity leading to relatively small ice crystals. Compared to the established process, drying times were reduced by half using the new processes. The drying time was, however, longer for syringes compared to vials due to the syringe design (long and slim). The differences in drying times were less pronounced for aggressive drying cycles. The proposed processes may help to considerably decrease investment costs into dual-chamber system fill-finish equipment. Dual-chamber syringes offer self-administration and home care use for freeze-dried pharmaceuticals. Only a few products have been launched in dual-chamber syringes so far-presumably due to their complex and costly drug product manufacturing process. In this paper two improved processes for freeze-drying pharmaceuticals in dual-chamber syringes are described. The major challenge of freeze-drying is to transfer heat through a vacuum. The proposed processes cope with this challenge by (1) freeze-drying the drug product in the syringe in an orientation in which the product is closest to the heat source, or (2) freeze-drying the drug product outside the syringe in a metal tube. The latter requires filling the freeze-dried product subsequently into the dual-chamber syringe. Both processes were very efficient and promised to achieve similar freeze-drying conditions for all dual-chamber syringes within one production run. The proposed processes may help to considerably decrease investment costs into dual-chamber syringe fill-finish equipment. © PDA, Inc. 2016.

Optimizing EEMCO guidance for the assessment of dry skin (xerosis) for pharmacies.

PubMed

Kang, B C; Kim, Y E; Kim, Y J; Chang, M J; Choi, H D; Li, K; Shin, W G

2014-02-01

People with dry skin (xerosis) are common in community pharmacies, but there is no consistent guidance for community pharmacists to evaluate and alleviate dry skin. Through evaluating any difference of the clinical scoring systems of EEMCO guidance between a dermatologist and pharmacists and the efficacy of moisturizers for the treatment of dry skin recommended by community pharmacists, we aim to validate a dry skin guidance through the help of community pharmacists. These results provide insight into how community pharmacists can help patients with dry skin. The clinical scoring systems of EEMCO guidance used in this study comprised analog scales, the overall dry skin score (ODS), and the specific symptom sum score (SRRC) system. All pictures of the dry skin scored by pharmacists were visually evaluated by a dermatologist. The efficacy of the moisturizers was determined by the difference of the scales on day 0 and on day 28. In this study, 387 patients with dry skin from 157 community pharmacies were evaluated by pharmacists. Visual scale with divisions, ODS and SRRC that were evaluated by pharmacists on day 0 and day 28 were moderately reliable by a dermatologist. All parameters of dry skin were significantly improved by the moisturizers which were recommended by community pharmacists on day 28. Visual scale, ODS and SRRC can be generally measured to evaluate dry skin in community pharmacies with moderate degree of reliability. This finding has possible applications for investigating the assessment of the community pharmacists on clinical scoring system of dry skin and moisturizers. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Design and Testing of Trace Contaminant Injection and Monitoring Systems

NASA Technical Reports Server (NTRS)

Broerman, Craig D.; Sweterlitsch, Jeff

2009-01-01

In support of the Carbon dioxide And Moisture Removal Amine Swing-bed (CAMRAS) testing, a contaminant injection system as well as a contaminant monitoring system has been developed by the Johnson Space Center Air Revitalization Systems (JSC-ARS) team. The contaminant injection system has been designed to provide trace level concentrations of contaminants generated by humans in a closed environment during space flight missions. The contaminant injection system continuously injects contaminants from three gas cylinders, two liquid reservoirs and three permeation ovens. The contaminant monitoring system has been designed to provide real time gas analysis with accurate flow, humidity and gas concentration measurements for collection during test. The contaminant monitoring system consists of an analytical real time gas analyzer, a carbon monoxide sensor, and an analyzer for ammonia and water vapor.

Design of modular control system for grain dryers

NASA Astrophysics Data System (ADS)

He, Gaoqing; Liu, Yanhua; Zu, Yuan

In order to effectively control the temperature of grain drying bin, grain ,air outlet as well as the grain moisture, it designed the control system of 5HCY-35 which is based on MCU to adapt to all grains drying conditions, high drying efficiency, long life usage and less manually. The system includes: the control module of the constant temperature and the temperature difference control in drying bin, the constant temperature control of heating furnace, on-line testing of moisture, variety of grain-circulation speed control and human-computer interaction interface. Spatial curve simulation, which takes moisture as control objectives, controls the constant temperature and the temperature difference in drying bin according to preset parameter by the user or a list to reduce the grains explosive to ensure the seed germination percentage. The system can realize the intelligent control of high efficiency and various drying, the good scalability and the high quality.

A lyophilized etoposide submicron emulsion with a high drug loading for intravenous injection: preparation, evaluation, and pharmacokinetics in rats.

PubMed

Chen, Hao; Shi, Shuai; Zhao, Mingming; Zhang, Ling; He, Haibing; Tang, Xing

2010-12-01

To develop a submicron emulsion for etoposide with a high drug loading capacity using a drug-phospholipid complex combined with drug freeze-drying techniques. An etoposide-phospholipid complex (EPC) was prepared and its structure was confirmed by X-ray diffraction and differential scanning calorimetry analysis. A freeze-drying technique was used to produce lyophilized etoposide emulsions (LEPE), and LEPE was investigated with regard to their appearance, particle size, and zeta potential. The pharmacokinetic study in vivo was determined by the UPLC/MS/MS system. It showed that EPC significantly improved the liposolubility of etoposide, indicating a high drug loading intravenous emulsion could be easily prepared by EPC. Moreover, the obtained loading of etoposide in the submicron emulsion was 3.0 mg/mL, which was three times higher than that of the previous liquid emulsions. The optimum cryoprotectant was trehalose (15%) in freeze-drying test. The median diameter, polydispersity index, and zeta potential of the optimum formulation of LEPE were 226.1 ± 5.1 nm, 0.107 ± 0.011, and -36.20 ± 1.13 mV, respectively. In addition, these parameters had no significant change during 6 months storage at 4 ± 2°C. The main pharmacokinetic parameters exhibited no significant differences between LEPE and etoposide commercial solution except for area under the concentration-time curve and clearance. The stable etoposide emulsion with a high drug loading was successfully prepared, indicating the amount of excipients such as the oil phase and emulsifiers significantly decreased following administration of the same dose of drug, effectively reducing the metabolism by patients while increasing their compliance. Therefore, LEPE has a great potential for clinical applications.

Simplifying sample pretreatment: application of dried blood spot (DBS) method to blood samples, including postmortem, for UHPLC-MS/MS analysis of drugs of abuse.

PubMed

Odoardi, Sara; Anzillotti, Luca; Strano-Rossi, Sabina

2014-10-01

The complexity of biological matrices, such as blood, requires the development of suitably selective and reliable sample pretreatment procedures prior to their instrumental analysis. A method has been developed for the analysis of drugs of abuse and their metabolites from different chemical classes (opiates, methadone, fentanyl and analogues, cocaine, amphetamines and amphetamine-like substances, ketamine, LSD) in human blood using dried blood spot (DBS) and subsequent UHPLC-MS/MS analysis. DBS extraction required only 100μL of sample, added with the internal standards and then three droplets (30μL each) of this solution were spotted on the card, let dry for 1h, punched and extracted with methanol with 0.1% of formic acid. The supernatant was evaporated and the residue was then reconstituted in 100μL of water with 0.1% of formic acid and injected in the UHPLC-MS/MS system. The method was validated considering the following parameters: LOD and LOQ, linearity, precision, accuracy, matrix effect and dilution integrity. LODs were 0.05-1ng/mL and LOQs were 0.2-2ng/mL. The method showed satisfactory linearity for all substances, with determination coefficients always higher than 0.99. Intra and inter day precision, accuracy, matrix effect and dilution integrity were acceptable for all the studied substances. The addition of internal standards before DBS extraction and the deposition of a fixed volume of blood on the filter cards ensured the accurate quantification of the analytes. The validated method was then applied to authentic postmortem blood samples. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Preparation of TPP-crosslinked chitosan microparticles by spray drying for the controlled delivery of progesterone intended for estrus synchronization in cattle.

PubMed

Helbling, Ignacio M; Busatto, Carlos A; Fioramonti, Silvana A; Pesoa, Juan I; Santiago, Liliana; Estenoz, Diana A; Luna, Julio A

2018-02-20

Planned reproduction in cattle involves regulation of estrous cycle and the use of artificial insemination. Cycle control includes the administration of exogenous progesterone during 5-8 days in a controlled manner allowing females to synchronize their ovulation. Several progesterone delivery systems are commercially available but they have several drawbacks. The aim of the present contribution was to evaluate chitosan microparticles entrapping progesterone as an alternative system. Microparticles were prepared by spray drying. The effect of formulation parameters and experimental conditions on particle features and delivery was studied. A mathematical model to predict progesterone plasma concentration in animals was developed and validated with experimental data. Microparticle size was not affected by formulation parameters but sphericity enhances as Tween 80 content increases and it impairs as TPP content rises. Z potential decreases as phosphate content rises. Particles remain stable in acidic solution but the addition of surfactant is required to stabilize dispersions in neutral medium. Encapsulation efficiencies was 69-75%. In vitro delivery studies showed burst and diffusion-controlled phases, being progesterone released faster at low pH. In addition, delivery extend in cows was affected mainly by particle size and hormone initial content, while the amount injected altered plasma concentration. Theoretical predictions with excellent accuracy were obtained. The mathematical model developed can help to find proper particle features to reach specific delivery rates in the animals. This not only save time, money and effort but also minimized experimentation with animals which is desired from an ethical point of view.

Cadaveric Assessment of Lip Injections: Locating the Serious Threats.

PubMed

Tansatit, Tanvaa; Apinuntrum, Prawit; Phetudom, Thavorn

2017-04-01

Lip augmentation could be a possible cause of blindness following filler injections. This study evaluated the risk by simulating clinical scenarios of marginal injections to the upper and lower lips and then evaluated the risk of vascular injuries. A 22G cannula was inserted bilaterally along the wet-dry junction of the upper and lower lip margins in fifteen cadavers, and then both lips were dissected to verify possible injuries to the superior and inferior labial arteries. The position of the labial arteries in the vermilion zone was documented to determine the appropriate injection technique. In the marginal injections to the lips, arterial injuries occurred at the medial segment of the vermilion zone of both the upper and lower lips, at the terminal part of the labial arteries or a distal branch. Considering arterial anatomy, the upper lip has a higher chance of arterial injury than the lower lip. The cannula should not be inserted in the submucosa as it is recommended to evert the vermilion because both the superior and inferior labial arteries are located in the submucosa of the medial and middle segments of the vermilion in all specimens. Awareness of the possibility of vascular injury is necessary during injections of the medial segments of the vermilion of the lips. Vermilion border and marginal injections are recommended for safe and effective lip augmentation. Deep injection around the oral commissure and submucosal injection of the medial and middle segments of the vermilion zone are prohibited because of the high risk of arterial injury. This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Engineering solutions to improve the removal of fecal indicator bacteria by bioinfiltration systems during intermittent flow of stormwater.

PubMed

Mohanty, Sanjay K; Torkelson, Andrew A; Dodd, Hanna; Nelson, Kara L; Boehm, Alexandria B

2013-10-01

Bioinfiltration systems facilitate the infiltration of urban stormwater into soil and reduce high flow events and flooding. Stormwater carries a myriad of pollutants including fecal indicator bacteria (FIB). Significant knowledge gaps exist about the ability of bioinfiltration systems to remove and retain FIB. The present study investigates the ability of model, simplified bioinfiltration systems containing quartz sand and iron oxide-coated quartz sand (IOCS) to remove two FIB (Enterococcus faecalis and Escherichia coli) suspended in synthetic stormwater with and without natural organic matter (NOM) as well as the potential for accumulated FIB to be remobilized during intermittent flow. The experiments were conducted in two phases: (1) the saturated columns packed with either sand or IOCS were contaminated by injecting stormwater with bacteria followed by injection of sterile stormwater and (2) the contaminated columns were subjected to intermittent infiltration of sterile stormwater preceded by a pause during which columns were either kept saturated or drained by gravity. During intermittent flow, fewer bacteria were released from the saturated column compared to the column drained by gravity: 12% of attached E. coli and 3% of attached Ent. faecalis were mobilized from the drained sand column compared to 3% of attached E. coli and 2% attached Ent. faecalis mobilized from the saturated sand column. Dry and wet cycles introduce moving air-water interfaces that can scour bacteria from grain surfaces. During intermittent flows, less than 0.2% of attached bacteria were mobilized from IOCS, which bound both bacteria irreversibly in the absence of NOM. Addition of NOM, however, increased bacterial mobilization from IOCS: 50% of attached E. coli and 8% of attached Ent. faecalis were released from IOCS columns during draining and rewetting. Results indicate that using geomedia such as IOCS that promote irreversible attachment of bacteria, and maintaining saturated condition, could minimize the mobilization of previous attached bacteria from bioinfiltration systems, although NOM may significantly decrease these benefits.

Discharge characteristics of a high speed fuel injection system

NASA Technical Reports Server (NTRS)

Matthews, Robertson

1925-01-01

Discussed here are some discharge characteristics of a fuel injection system intended primarily for high speed service. The system consisted of a cam actuated fuel pump, a spring loaded automatic injection valve, and a connecting tube.

Hydrogel Nanoparticles from Supercritical Technology for Pharmaceutical and Seismological Applications

NASA Astrophysics Data System (ADS)

Hemingway, Melinda Graham

This research focuses on hydrogel nanoparticle formation using miniemulsion polymerization and supercritical carbon dioxide. Hydrogel nanopowder is produced by a novel combination of inverse miniemulsion polymerization and supercritical drying (MPSD) methods. Three drying methods of miniemulsions are examined: (1) a conventional freeze drying technique, and (2) two supercritical drying techniques: (2a) supercritical fluid injection into miniemulsions, and (2b) the polymerized miniemulsion injection into supercritical fluid. Method 2b can produce non-agglomerated hydrogel nanoparticles that are free of solvent or surfactant (Chapter 2). The optimized MPSD method was applied for producing an extended release drug formulation with mucoadhesive properties. Drug nanoparticles of mesalamine, were produced using supercritical antisolvent technology and encapsulation within two hydrogels, polyacrylamide and poly(acrylic acid-co-acrylamide). The encapsulation efficiency and release profile of drug nanoparticles is compared with commercial ground mesalamine particles. The loading efficiency is influenced by morphological compatibility (Chapter 3). The MPSD method was extended for encapsulation of zinc oxide nanoparticles for UV protection in sunscreens (Chapter 4). ZnO was incorporated into the inverse miniemulsion during polymerization. The effect of process parameters are examined on absorbency of ultraviolet light and transparency of visible light. For use of hydrogel nanoparticles in a seismological application, delayed hydration is needed. Supercritical methods extend MPSD so that a hydrophobic coating can be applied on the particle surface (Chapter 5). Multiple analysis methods and coating materials were investigated to elucidate compatibility of coating material to polyacrylamide hydrogel. Coating materials of poly(lactide), poly(sulphone), poly(vinyl acetate), poly(hydroxybutyrate), Geluice 50-13, Span 80, octadecyltrichlorosilane, and perfluorobutane sulfate (PFBS) were tested, out of which Gelucire, perfluorobutane sulfate, and poly(vinyl acetate) materials were able to provide some coating and perfluorobutane sulfate, poly(lactide), poly(vinyl acetate) delayed hydration of hydrogel particles, but not to a sufficient extent. The interactions of the different materials with the hydrogel are examined based on phenomena observed during the production processes and characterization of the particles generated. This work provides understanding into the interactions of polyacrylamide hydrogel particles both internally by encapsulation and externally by coating.

Reductant injection and mixing system

DOEpatents

Reeves, Matt; Henry, Cary A.; Ruth, Michael J.

2016-02-16

A gaseous reductant injection and mixing system is described herein. The system includes an injector for injecting a gaseous reductant into an exhaust gas stream, and a mixer attached to a surface of the injector. The injector includes a plurality of apertures through which the gaseous reductant is injected into an exhaust gas stream. The mixer includes a plurality of fluid deflecting elements.

Microcontroller-driven fluid-injection system for atomic force microscopy.

PubMed

Kasas, S; Alonso, L; Jacquet, P; Adamcik, J; Haeberli, C; Dietler, G

2010-01-01

We present a programmable microcontroller-driven injection system for the exchange of imaging medium during atomic force microscopy. Using this low-noise system, high-resolution imaging can be performed during this process of injection without disturbance. This latter circumstance was exemplified by the online imaging of conformational changes in DNA molecules during the injection of anticancer drug into the fluid chamber.

Life Cycle Cost of Solar Biomass Hybrid Dryer Systems for Cashew Drying of Nuts in India

NASA Astrophysics Data System (ADS)

Dhanushkodi, Saravanan; Wilson, Vincent H.; Sudhakar, Kumarasamy

2015-12-01

Cashew nut farming in India is mostly carried out in small and marginal holdings. Energy consumption in the small scale cashew nut processing industry is very high and is mainly due to the high energy consumption of the drying process. The drying operation provides a lot of scope for energy saving and substitutions of other renewable energy sources. Renewable energy-based drying systems with loading capacity of 40 kg were proposed for application in small scale cashew nut processing industries. The main objective of this work is to perform economic feasibility of substituting solar, biomass and hybrid dryer in place of conventional steam drying for cashew drying. Four economic indicators were used to assess the feasibility of three renewable based drying technologies. The payback time was 1.58 yr. for solar, 1.32 for biomass and 1.99 for the hybrid drying system, whereas as the cost-benefit estimates were 5.23 for solar, 4.15 for biomass and 3.32 for the hybrid system. It was found that it is of paramount importance to develop solar biomass hybrid dryer for small scale processing industries.

Infrared Drying as a Potential Alternative to Convective Drying for Biltong Production.

PubMed

Cherono, Kipchumba; Mwithiga, Gikuru; Schmidt, Stefan

2016-06-03

Two infrared systems set at an intensity of 4777 W/m 2 with peak emission wavelengths of 2.5 and 3.5 µm were used to produce biltong by drying differently pre-treated meat. In addition to meat texture and colour, the microbial quality of the biltong produced was assessed by quantifying viable heterotrophic microorganisms using a most probable number (MPN) method and by verifying the presence of presumptive Escherichia coli in samples produced using infrared and conventional convective drying. The two infrared drying systems reduced the heterotrophic microbial burden from 5.11 log 10 MPN/g to 2.89 log 10 MPN/g (2.5 µm) and 3.42 log 10 MPN/g (3.5 µm), respectively. The infrared systems achieved an up to one log higher MPN/g reduction than the convective system. In biltong samples produced by short wavelength (2.5 µm) infrared drying, E. coli was not detectable. This study demonstrates that the use of short wavelength infrared drying is a potential alternative to conventional convective drying by improving the microbiological quality of biltong products while at the same time delivering products of satisfactory quality.

40 CFR 49.4164 - Construction and operational control measures.

Code of Federal Regulations, 2014 CFR

2014-07-01

... closed-vent system to: (i) An operating system designed to recover and inject all the produced natural... through a closed-vent system to: (i) An operating system designed to recover and inject the natural gas... operating system designed to recover and inject natural gas becomes temporarily infeasible and there is no...

40 CFR 49.4164 - Construction and operational control measures.

Code of Federal Regulations, 2013 CFR

2013-07-01

... closed-vent system to: (i) An operating system designed to recover and inject all the produced natural... through a closed-vent system to: (i) An operating system designed to recover and inject the natural gas... operating system designed to recover and inject natural gas becomes temporarily infeasible and there is no...

Influence of pre-injection control parameters on main-injection fuel quantity for an electronically controlled double-valve fuel injection system of diesel engine

NASA Astrophysics Data System (ADS)

Song, Enzhe; Fan, Liyun; Chen, Chao; Dong, Quan; Ma, Xiuzhen; Bai, Yun

2013-09-01

A simulation model of an electronically controlled two solenoid valve fuel injection system for a diesel engine is established in the AMESim environment. The accuracy of the model is validated through comparison with experimental data. The influence of pre-injection control parameters on main-injection quantity under different control modes is analyzed. In the spill control valve mode, main-injection fuel quantity decreases gradually and then reaches a stable level because of the increase in multi-injection dwell time. In the needle control valve mode, main-injection fuel quantity increases with rising multi-injection dwell time; this effect becomes more obvious at high-speed revolutions and large main-injection pulse widths. Pre-injection pulse width has no obvious influence on main-injection quantity under the two control modes; the variation in main-injection quantity is in the range of 1 mm3.

Volatile compound changes during shelf life of dried Boletus edulis: comparison between SPME-GC-MS and PTR-ToF-MS analysis.

PubMed

Aprea, Eugenio; Romano, Andrea; Betta, Emanuela; Biasioli, Franco; Cappellin, Luca; Fanti, Marco; Gasperi, Flavia

2015-01-01

Drying process is commonly used to allow long time storage of valuable porcini mushrooms (Boletus edulis). Although considered a stable product dried porcini flavour changes during storage. Monitoring of volatile compounds during shelf life may help to understand the nature of the observed changes. In the present work two mass spectrometric techniques were used to monitor the evolution of volatile compounds during commercial shelf life of dried porcini. Solid phase microextraction (SPME) coupled to gas chromatography - mass spectrometry (GC-MS) allowed the identification of 66 volatile compounds, 36 of which reported for the first time, monitored during the commercial shelf life of dried porcini. Proton transfer reaction - time of flight - mass spectrometry (PTR-ToF-MS) , a direct injection mass spectrometric technique, was shown to be a fast and sensitive instrument for the general monitoring of volatile compound evolution during storage of dried porcini. Furthermore, PTR-ToF-MS grants access to compounds whose determination would otherwise require lengthy pre-concentration and/or derivatization steps such as ammonia and small volatile amines. The two techniques, both used for the first time to study dried porcini, provided detailed description of time evolution of volatile compounds during shelf life. Alcohols, aldehydes, ketones and monoterpenes diminish during the storage while carboxylic acids, pyrazines, lactones and amines increase. The storage temperature modifies the rate of the observed changes influencing the final quality of the dried porcini. We showed the advantages of both techniques, suggesting a strategy to be adopted to follow time evolution of volatile compounds in food products during shelf life, based on the identification of compounds by GC-MS and the rapid time monitoring by PTR-ToF-MS measurements in order to maximize the advantages of both techniques. Copyright © 2015 John Wiley & Sons, Ltd.

Analysis of maizena drying system using temperature control based fuzzy logic method

NASA Astrophysics Data System (ADS)

Arief, Ulfah Mediaty; Nugroho, Fajar; Purbawanto, Sugeng; Setyaningsih, Dyah Nurani; Suryono

2018-03-01

Corn is one of the rice subtitution food that has good potential. Corn can be processed to be a maizena, and it can be used to make type of food that has been made from maizena, viz. Brownies cake, egg roll, and other cookies. Generally, maizena obtained by drying process carried out 2-3 days under the sun. However, drying process not possible during the rainy season. This drying process can be done using an automatic drying tool. This study was to analyze the design result and manufacture of maizena drying system with temperature control based fuzzylogic method. The result show that temperature of drying system with set point 40°C - 60°C work in suitable condition. The level of water content in 15% (BSN) and temperatureat 50°C included in good drying process. Time required to reach the set point of temperature in 50°C is 7.05 minutes. Drying time for 500 gr samples with temperature 50°C and power capacity 127.6 watt was 1 hour. Based on the result, drying process using temperature control based fuzzy logic method can improve energy efficiency than the conventional method of drying using a direct sunlight source with a temperature that cannot be directly controlled by human being causing the quality of drying result of flour is erratic.

Dynamics of clogging in drying porous media

NASA Astrophysics Data System (ADS)

Kaplan, C. Nadir; Mahadevan, L.

2014-11-01

Drying in porous media pervades a range of phenomena from brine evaporation arrested in porous bricks, causing efflorescence, i.e. salt aggregation on the surface where vapor leaves the medium, to clogging of reservoir rocks via salt precipitation when carbon dioxide is injected for geological storage. During the process of drying, the permeability and porosity of the medium may change due to the solute accumulation as a function of the particle concentration, in turn affecting the evaporation rate and the dynamics of the fluid flow imposed by it. To examine the dynamics of these coupled quantities, we develop a multiphase model of the particulate flow of a saline suspension in a porous medium, induced by evaporation. We further provide dimensional arguments as to how the salt concentration and the resulting change in permeability determine the transition between efflorescence and salt precipitation in the bulk. This research was supported by the Air Force Office of Scientific Research (AFOSR) under Award FA9550-09-1-0669-DOD35CAP and the Kavli Institute for Bionano Science and Technology at Harvard University.

Monitoring of subsurface injection of wastes, Florida

USGS Publications Warehouse

Vecchioli, John

1979-01-01

Injection of waste liquids into Florida's subsurface is physically feasible in many places but should be accompanied by monitoring of the waste-receiving aquifer system in addition to the injection facility. Monitoring of the interaction of factors including hydrogeologic conditions, well construction, waste volumes and characteristics, and potable-water sources is desirable to assure that fresh-water resources are not being adversely affected. An effective aquifer-system monitoring program includes on-site wells located close to an injection well and open to the next-higher permeable stratum, satellite wells located hundreds to several thousands of feet from an injection well and open to the receiving aquifer, and regional wells located miles from individual injection wells and open to the receiving aquifer. An extensive aquifer-system monitoring program associated with two waste-injection facilities near Pensacola, Florida, has provided data which have aided hydrologists to understand the aquifer system's response to the injection and, accordingly, to evaluate the potential for affecting the area's fresh-water resources.

Steam atmosphere drying exhaust steam recompression system

DOEpatents

Becker, F.E.; Smolensky, L.A.; Doyle, E.F.; DiBella, F.A.

1994-03-08

This invention relates to a heated steam atmosphere drying system comprising dryer in combination with an exhaust recompression system which is extremely energy efficient and eliminates dangers known to air dryers. The system uses superheated steam as the drying medium, which recirculates through the system where its heat of evaporation and heat of compression is recovered, thereby providing a constant source of heat to the drying chamber. The dryer has inlets whereby feedstock and superheated steam are fed therein. High heat transfer and drying rates are achieved by intimate contact of the superheated steam with the particles being dried. The dryer comprises a vessel which enables the feedstock and steam to enter and recirculate together. When the feedstock becomes dry it will exit the dryer with the steam and become separated from the steam through the use of a curvilinear louver separator (CLS). The CLS enables removal of fine and ultrafine particles from the dryer. Water vapor separated from the particles in the CLS as superheated steam, may then be recovered and recirculated as steam through the use of a compressor to either directly or indirectly heat the dryer, and a heat exchanger or a heater to directly provide heat to the dryer. This system not only provides a very efficient heat transfer system but results in a minimum carry-over of ultrafine particles thereby eliminating any explosive hazard. 17 figures.

Steam atmosphere drying exhaust steam recompression system

DOEpatents

Becker, Frederick E.; Smolensky, Leo A.; Doyle, Edward F.; DiBella, Francis A.

1994-01-01

This invention relates to a heated steam atmosphere drying system comprising dryer in combination with an exhaust recompression system which is extremely energy efficient and eliminates dangers known to air dryers. The system uses superheated steam as the drying medium, which recirculated through the system where its heat of evaporation and heat of compression is recovered, thereby providing a constant source of heat to the drying chamber. The dryer has inlets whereby feedstock and superheated steam are fed therein. High heat transfer and drying rates are achieved by intimate contact of the superheated steam with the particles being dried The dryer comprises a vessel which enables the feedstock and steam to enter recirculate together. When the feedstock becomes dry it will exit the dryer with the steam and become separated from the steam through the use of a curvilinear louver separator (CLS). The CLS enables removal of fine and ultrafine particles from the dryer. Water vapor separated from the particles in the CLS as superheated steam, may then be recovered and recirculated as steam through the use of a compressor to either directly or indirectly heat the dryer, and a heat exchanger or a heater to directly provide heat to the dryer. This system not only provides a very efficient heat transfer system but results in a minimum carry-over of ultrafine particles thereby eliminating any explosive hazard.

Economics of water injected air screw compressor systems

NASA Astrophysics Data System (ADS)

Venu Madhav, K.; Kovačević, A.

2015-08-01

There is a growing need for compressed air free of entrained oil to be used in industry. In many cases it can be supplied by oil flooded screw compressors with multi stage filtration systems, or by oil free screw compressors. However, if water injected screw compressors can be made to operate reliably, they could be more efficient and therefore cheaper to operate. Unfortunately, to date, such machines have proved to be insufficiently reliable and not cost effective. This paper describes an investigation carried out to determine the current limitations of water injected screw compressor systems and how these could be overcome in the 15-315 kW power range and delivery pressures of 6-10 bar. Modern rotor profiles and approach to sealing and cooling allow reasonably inexpensive air end design. The prototype of the water injected screw compressor air system was built and tested for performance and reliability. The water injected compressor system was compared with the oil injected and oil free compressor systems of the equivalent size including the economic analysis based on the lifecycle costs. Based on the obtained results, it was concluded that water injected screw compressor systems could be designed to deliver clean air free of oil contamination with a better user value proposition than the oil injected or oil free screw compressor systems over the considered range of operations.

Parametric performance analysis of steam-injected gas turbine with a thermionic-energy-converter-lined combustor

NASA Technical Reports Server (NTRS)

Choo, Y. K.; Burns, R. K.

1982-01-01

The performance of steam-injected gas turbines having combustors lined with thermionic energy converters (STIG/TEC systems) was analyzed and compared with that of two baseline systems; a steam-injected gas turbine (without a TEC-lined combustor) and a conventional combined gas turbine/steam turbine cycle. Common gas turbine parameters were assumed for all of the systems. Two configurations of the STIG/TEC system were investigated. In both cases, steam produced in an exhaust-heat-recovery boiler cools the TEC collectors. It is then injected into the gas combustion stream and expanded through the gas turbine. The STIG/TEC system combines the advantage of gas turbine steam injection with the conversion of high-temperature combustion heat by TEC's. The addition of TEC's to the baseline steam-injected gas turbine improves both its efficiency and specific power. Depending on system configuration and design parameters, the STIG/TEC system can also achieve higher efficiency and specific power than the baseline combined cycle.

Design of Tomato Drying System by Utilizing Brine Geothermal

NASA Astrophysics Data System (ADS)

Afuar, W.; Sibarani, B.; Abdurrahman, G.; Hendrarsakti, J.

2016-09-01

Cultivation of tomato plants in Indonesia has been started since 1961.Tomatoes generally will rot in three days if left on storage. Moreover, low quality tomatoes have cheaper price. After harvested, tomatoes need to be treated by drying process so it can last longer. Energy for drying tomatoes can be obtained by utilizing heat from geothermal brine. Purpose of this research is to design a tomato drying system by extracting heat of geothermal brine from separator with certain flow rate to heat up water by using a heat exchanger. Furthermore, this water will be used to heat up the surrounding air which is circulated by blower system to heat up the tomatoes chamber. Tomatoes drying process needs temperature range of 50-70°C to evaporate water content from 95.7% to 26%. After that treatment, the tomatoes are expected to have better durability. The objective of this study is to determine the quantity of hot brine which is needed for drying tomatoes and to design a drying system so that tomatoes can last longer.

Penetration and Duration of Fuel Sprays from a Pump Injection System

NASA Technical Reports Server (NTRS)

Rothrock, A M; Marsh, E T

1931-01-01

High-speed motion pictures were taken of individual fuel sprays from a pump injection system. The changes in the spray-tip penetration with changes in the pump speed, injection-valve opening and closing pressures, discharge-orifice area, injection-tube length and diameter, and pump throttle setting were measured. In addition, the effects of the variables on the time lag and duration of injection can be controlled by the dimensions of the injection tube, the area of the discharge orifice, and the injection-valve opening and closing pressures.

Investigation of the chemical pathway of gaseous nitrogen dioxide formation during flue gas desulfurization with dry sodium bicarbonate injection

NASA Astrophysics Data System (ADS)

Stein, Antoinette Weil

The chemical reaction pathway for the viable flue gas desulfurization process, dry sodium bicarbonate injection, was investigated to mitigate undesirable plume discoloration. Based on a foundation of past findings, a simplified three-step reaction pathway was hypothesized for the formation of the plume-discoloring constituent, NO2. As the first step, it was hypothesized that sodium sulfite formed by sodium bicarbonate reaction with flue gas SO 2. As the second step, it was hypothesized that sodium nitrate formed by sodium sulfite reaction with flue gas NO. And as the third step, it was hypothesized that NO2 and sodium sulfate formed by sodium nitrate reaction with SO2. The second and third hypothesized steps were experimentally investigated using an isothermal fixed bed reactor. As reported in the past, technical grade sodium sulfite was found to be un-reactive with NO and O2. Freshly prepared sodium sulfite, maintained unexposed to moist air, was shown to react with NO and O2 resulting in a mixture of sodium nitrite and sodium nitrate together with a significant temperature rise. This reaction was found to proceed only when oxygen was present in the flue gas. As reported in the past, technical grade sodium nitrate was shown to be un-reactive with SO2. But freshly formed sodium nitrate kept unexposed to humidity was found to be reactive with SO2 and O 2 resulting in the formation of NO2 and sodium sulfate polymorphic Form I. The NO2 formation by this reaction was shown to be temperature dependent with maximum formation at 175°C. Plume mitigation methods were studied based on the validated three-step reaction pathway. Mitigation of NO2 was exhibited by limiting oxygen concentration in the flue gas to a level below 5%. It was also shown that significant NO2 mitigation was achieved by operating below 110°C or above 250°C. An innovative NO2 mitigation method was patented as a result of the findings of this study. The patented process incorporated a process step of sodium sulfite injection to remove flue gas NO prior to sodium bicarbonate injection.

Feasibility of hydrogel fiducial markers for in vivo proton range verification using PET

NASA Astrophysics Data System (ADS)

Cho, Jongmin; Campbell, Patrick; Wang, Min; Alqathami, Mamdooh; Mawlawi, Osama; Kerr, Matthew; Cho, Sang Hyun

2016-03-01

Biocompatible/biodegradable hydrogel polymers were immersed in 18O-enriched water and 16O-water to create 18O-water hydrogels and 16O-water hydrogels. In both cases, the hydrogels were made of ~91 wt% water and ~9 wt% polymer. In addition, 5-8 μm Zn powder was suspended in 16O-water and 18O-enriched water and cross-linked with hydrogel polymers to create Zn/16O-water hydrogels (30/70 wt%, ~9 wt% polymer) and Zn/18O-water hydrogels (10/90 wt%), respectively. A block of extra-firm ‘wet’ tofu (12.3  ×  8.8  ×  4.9 cm, ρ  ≈  1.05 g cm-3) immersed in water was injected with Zn/16O-water hydrogels (0.9 ml each) at four different depths using an 18-gauge needle. Similarly, Zn/18O-water hydrogels (0.9 ml) were injected into a second tofu phantom. As a reference, both 16O-water hydrogels (1.8 ml) and 18O-water hydrogels (0.9 ml) in Petri dishes were irradiated in a ‘dry’ environment. The hydrogels in the wet tofu phantoms and dry Petri dishes were scanned via CT and images were used for treatment planning. Then, they were positioned at the proton distal dose fall-off region and irradiated (2 Gy) followed by PET/CT imaging. Notably high PET signals were observed only in 18O-water hydrogels in the dry environment. The visibility of the Zn/16O-water hydrogels injected into the tofu phantom was outstanding in CT images, but these hydrogels provided no noticeable PET signals. The visibility of the Zn/18O-water hydrogels in the wet tofu were excellent on CT and moderate on PET; however, the PET signals were weaker than those in the dry environment, possibly owing to 18O-water leaching out. The hydrogel markers studied here could be used to develop universal PET/CT fiducial markers. Their PET visibility (attributed more to activated 18O-water than Zn) after proton irradiation can be used for proton therapy/range verification. More investigation is needed to slow down the leaching of 18O-water.

Therapeutic efficacy of fibroblast growth factor 10 in a rabbit model of dry eye.

PubMed

Zheng, Wenjing; Ma, Mingming; Du, Ergang; Zhang, Zhengwei; Jiang, Kelimu; Gu, Qing; Ke, Bilian

2015-11-01

The aim of the present study was to investigate the therapeutic efficacy of fibroblast growth factor 10 (FGF10) in the promotion of healing, survival and expression of mucin in corneal epithelial cells in a rabbit dry eye model. A total of 12 healthy female New Zealand white rabbits were divided randomly into three groups. The lacrimal glands were injected with saline either alone (normal control group) or with concanavalin A (Con A), with either topical phosphate‑buffered saline (PBS; PBS control group) or 25 µg/ml FGF10 (FGF10 treatment group). Lacrimal gland inflammation, tear function, corneal epithelial cell integrity, cell apoptosis and mucin expression were subsequently assessed. Lacrimal gland tissue biopsies were performed in conjunction with histology and electron microscopy observations. Tear meniscus height (TMH) and tear meniscus area (TMA) were measured using Fourier domain‑optical coherence tomography. Tear membrane break‑up time (TBUT) was also assessed and corneal fluorescein staining was performed. The percentages of apoptotic corneal and conjunctival (Cj) epithelial cells (ECs) were counted using a terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling method. The mRNA expression levels of Muc1 were determined using reverse transcription‑quantitative polymerase chain reaction analyses. The TMH and TMA values of the PBS and treatment groups were found to be significantly reduced, compared with those of the normal control group 3 days after Con A injection. However, the TMH and TMA of the FGF10 treatment group were higher, compared with those of the PBS group 3 and 7 days after treatment, respectively. Furthermore, the FGF10 treatment group exhibited prolonged TBUT, reduced corneal fluorescein staining and repaired epithelial cell ultrastructure7 days after treatment. The percentages of apoptotic corneal‑ and Cj‑ECs in the FGF10 treatment group were significantly reduced, compared with those in the PBS group. FGF10 significantly induced the mRNA expression of Muc1 in the corneal epithelial cells, compared with the normal control group, and induced higher mRNA expression levels of Muc1 in the Cj‑ECs, compared with the PBS control group. In the present study, the rabbit dry eye model was successfully established 3 days after lacrimal gland Con A injection. FGF10 eye drops increased TMH and TMA, promoted corneal epithelial healing, reduced apoptosis of the corneal- and Cj-ECs and led to increased expression of Muc1.

Integrating low-NO{sub x} burners, overfire air, and selective non-catalytic reduction on a utility coal-fired boiler

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hunt, T.; Muzio, L.; Smith, R.

1995-05-01

Public Service Company of Colorado (PSCo), in cooperation with the US Department of Energy (DOE) and the Electric Power Research Institute (EPRI), is testing the Integrated Dry NO{sub x}/SO{sub 2} Emissions Control system. This system combines low-NO{sub x} burners, overfire air, selective non-catalytic reduction (SNCR), and dry sorbent injection with humidification to reduce by up to 70% both NO{sub x} and SO{sub 2} emissions from a 100 MW coal-fired utility boiler. The project is being conducted at PSCo`s Arapahoe Unit 4 located in Denver, Colorado as part of the DOE`s Clean Coal Technology Round 3 program. The urea-based SNCR system,more » supplied by Noell, Inc., was installed in late 1991 and was tested with the unmodified boiler in 1992. At full load, it reduced NO{sub x} emissions by about 35% with an associated ammonia slip limit of 10 ppm. Babcock & Wilcox XLS{reg_sign} burners and a dual-zone overfire air system were retrofit to the top-fired boiler in mid-1992 and demonstrated a NO{sub x} reduction of nearly 70% across the load range. Integrated testing of the combustion modifications and the SNCR system were conducted in 1993 and showed that the SNCR system could reduce NO{sub x} emissions by an additional 45% while maintaining 10 ppm of ammonia slip limit at full load. Lower than expect4ed flue-gas temperatures caused low-load operation to be less effective than at high loads. NO{sub x} reduction decreased to as low as 11% at 60 MWe at an ammonia slip limit of 10 ppm. An ammonia conversion system was installed to improve performance at low loads. Other improvements to increase NO{sub x} removal at low-loads are planned. The combined system of combustion modifications and SNCR reduced NO{sub x} emissions by over 80% from the original full-load baseline. 11 figs.« less

Effects of Direct Renin Blockade on Renal & Systemic Hemodynamics and on RAAS Activity, in Weight Excess and Hypertension: A Randomized Clinical Trial.

PubMed

Kwakernaak, A J; Roksnoer, L C; Lambers Heerspink, H J; van den Berg-Garrelds, I; Lochorn, G A; van Embden Andres, J H; Klijn, M A; Kobori, H; Danser, A H J; Laverman, G D; Navis, G J

2017-01-01

The combination of weight excess and hypertension significantly contributes to cardiovascular risk and progressive kidney damage. An unfavorable renal hemodynamic profile is thought to contribute to this increased risk and may be ameliorated by direct renin inhibition (DRI). The aim of this trial was to assess the effect of DRI on renal and systemic hemodynamics and on RAAS activity, in men with weight excess and hypertension. A randomized, double-blind, cross-over clinical trial to determine the effect of DRI (aliskiren 300 mg/day), with angiotensin converting enzyme inhibition (ACEi; ramipril 10 mg/day) as a positive control, on renal and systemic hemodynamics, and on RAAS activity (n = 15). Mean (SEM) Glomerular filtration rate (101 (5) mL/min/1.73m2) remained unaffected by DRI or ACEi. Effective renal plasma flow (ERPF; 301 (14) mL/min/1.73m2) was increased in response to DRI (320 (14) mL/min/1.73m2, P = 0.012) and ACEi (317 (15) mL/min/1.73m2, P = 0.045). Filtration fraction (FF; 34 (0.8)%) was reduced by DRI only (32 (0.7)%, P = 0.044). Mean arterial pressure (109 (2) mmHg) was reduced by DRI (101 (2) mmHg, P = 0.008) and ACEi (103 (3) mmHg, P = 0.037). RAAS activity was reduced by DRI and ACEi. Albuminuria (20 [9-42] mg/d) was reduced by DRI only (12 [5-28] mg/d, P = 0.030). In men with weight excess and hypertension, DRI and ACEi improved renal and systemic hemodynamics. Both DRI and ACEi reduced RAAS activity. Thus, DRI provides effective treatment in weight excess and hypertension. Dutch trial register, registration number: 2532 www.trialregister.nl.

An alternative local anaesthesia technique to reduce pain in paediatric patients during needle insertion.

PubMed

Lee, S H; Lee, N Y

2013-06-01

Pain control, which is necessary during most dental procedures, is administered by injecting a local anaesthetic. Because the injection itself can be painful, the procedure via which pain is reduced warrants continued investigation. Only a few studies regarding the reaction of children to dental needle insertion without the use of topical anaesthetics have been reported. This study was conducted to evaluate the efficacy of the local anaesthetic procedure without topical application as compared to the conventional insertion technique for alleviating pain in children receiving local anaesthesia injections. For the alternative injection procedure, the dentist quickly and gently pulled or pushed the clean and dried loose tissue at the injection site over the tip of the needle to a depth of 1 to 1.5 mm. When the end of the bevel of the needle tip entered the tissue, a few drops of solution were released, after which the needle was advanced to its proper and intended depth to continue anaesthetic release. There was a significant difference regarding the pain response between the alternative insertion technique (less painful) and the conventional one according to Sound, Eye, and Motor (SEM) scale ratings (P < 0.000). No significant difference was observed in the response between the maxilla and mandible, nor between boys and girls, between the conventional and alternative techniques. This alternative technique can reduce discomfort in paediatric dental patients and allow the clinician to administer a superficial local anaesthesia injection before the needle is advanced into deeper tissue. This technique is simple, quick, devoid of additional costs, and potentially more effective than the conventional needle insertion method.

Dual fuel injection piggyback controller system

NASA Astrophysics Data System (ADS)

Muji, Siti Zarina Mohd.; Hassanal, Muhammad Amirul Hafeez; Lee, Chua King; Fawzi, Mas; Zulkifli, Fathul Hakim

2017-09-01

Dual-fuel injection is an effort to reduce the dependency on diesel and gasoline fuel. Generally, there are two approaches to implement the dual-fuel injection in car system. The first approach is changing the whole injector of the car engine, the consequence is excessive high cost. Alternatively, it also can be achieved by manipulating the system's control signal especially the Electronic Control Unit (ECU) signal. Hence, the study focuses to develop a dual injection timing controller system that likely adopted to control injection time and quantity of compressed natural gas (CNG) and diesel fuel. In this system, Raspberry Pi 3 reacts as main controller unit to receive ECU signal, analyze it and then manipulate its duty cycle to be fed into the Electronic Driver Unit (EDU). The manipulation has changed the duty cycle to two pulses instead of single pulse. A particular pulse mainly used to control injection of diesel fuel and another pulse controls injection of Compressed Natural Gas (CNG). The test indicated promising results that the system can be implemented in the car as piggyback system. This article, which was originally published online on 14 September 2017, contained an error in the acknowledgment section. The corrected acknowledgment appears in the Corrigendum attached to the pdf.

Description and cost analysis of a deluge dry/wet cooling system.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wiles, L.E.; Bamberger, J.A.; Braun, D.J.

1978-06-01

The use of combined dry/wet cooling systems for large base-load power plants offers the potential for significant water savings as compared to evaporatively cooled power plants and significant cost savings in comparison to dry cooled power plants. The results of a detailed engineering and cost study of one type of dry/wet cooling system are described. In the ''deluge'' dry/wet cooling method, a finned-tube heat exchanger is designed to operate in the dry mode up to a given ambient temperature. To avoid the degradation of performance for higher ambient temperatures, water (the delugeate) is distributed over a portion of the heatmore » exchanger surface to enhance the cooling process by evaporation. The deluge system used in this study is termed the HOETERV system. The HOETERV deluge system uses a horizontal-tube, vertical-plate-finned heat exchanger. The delugeate is distributed at the top of the heat exchanger and is allowed to fall by gravity in a thin film on the face of the plate fin. Ammonia is used as the indirect heat transfer medium between the turbine exhaust steam and the ambient air. Steam is condensed by boiling ammonia in a condenser/reboiler. The ammonia is condensed in the heat exchanger by inducing airflow over the plate fins. Various design parameters of the cooling system have been studied to evaluate their impact on the optimum cooling system design and the power-plant/utility-system interface. Annual water availability was the most significant design parameter. Others included site meteorology, heat exchanger configuration and air flow, number and size of towers, fan system design, and turbine operation. It was concluded from this study that the HOETERV deluge system of dry/wet cooling, using ammonia as an intermediate heat transfer medium, offers the potential for significant cost savings compared with all-dry cooling, while achieving substantially reduced water consumption as compared to an evaporatively cooled power plant. (LCL)« less

Efficacy of a Fatty Acids Dietary Supplement in a Polyethylene Glycol-Induced Mouse Model of Retinal Degeneration

PubMed Central

Locri, Filippo; Lardner, Emma; Kvanta, Anders; Rusciano, Dario; Bagnoli, Paola

2017-01-01

Current knowledge of the benefits of nutrition supplements for eye pathologies is based largely on the use of appropriate animal models, together with defined dietary supplementation. Here, C57BL6 mice were subretinally injected with polyethylene glycol (PEG)-400, an established model of retinal degeneration with a dry age-related macular degeneration (AMD)-like phenotype, an eye pathology that lacks treatment. In response to PEG-400, markers of the complement system, angiogenesis, inflammation, gliosis, and macrophage infiltration were upregulated in both retinas and retinal pigment epithelium (RPE)/choroids, whereas dietary supplementation with a mixture based on fatty acids counteracted their upregulation. Major effects include a reduction of inflammation, in both retinas and RPE/choroids, and an inhibition of macrophage infiltration in the choroid, yet not in the retina, suggesting a targeted action through the choroidal vasculature. Histological analysis revealed a thinning of the outer nuclear layer (ONL), together with dysregulation of the epithelium layer in response to PEG-400. In addition, immunohistofluorescence demonstrated Müller cell gliosis and macrophage infiltration into subretinal tissues supporting the molecular findings. Reduced ONL thickness, gliosis, and macrophage infiltration were counteracted by the diet supplement. The present data suggest that fatty acids may represent a useful form of diet supplementation to prevent or limit the progression of dry AMD. PMID:28961167

The Development and Implementation of a Cryogenic Pressure Sensitive Paint System in the National Transonic Facility

NASA Technical Reports Server (NTRS)

Watkins, A. Neal; Leighty, Bradley D.; Lipford, William E.; Oglesby, Donald M.; Goodman, Kyle Z.; Goad, William K.; Goad, Linda R.; Massey, Edward A.

2009-01-01

The Pressure Sensitive Paint (PSP) method was used to measure global surface pressures on a model at full-scale flight Reynolds numbers. In order to achieve these conditions, the test was carried out at the National Transonic Facility (NTF) operating under cryogenic conditions in a nitrogen environment. The upper surface of a wing on a full-span 0.027 scale commercial transport was painted with a porous PSP formulation and tested at 120K. Data was acquired at Mach 0.8 with a total pressure of 200 kPa, resulting in a Reynolds number of 65 x 106/m. Oxygen, which is required for PSP operation, was injected using dry air so that the oxygen concentration in the flow was approximately 1535 ppm. Results show qualitative agreement with expected results. This preliminary test is the first time that PSP has been successfully deployed to measure global surface pressures at cryogenic condition in the NTF. This paper will describe the system as installed, the results obtained from the test, as well as proposed upgrades and future tests.

An Injectable, Self-Healing Hydrogel to Repair the Central Nervous System.

PubMed

Tseng, Ting-Chen; Tao, Lei; Hsieh, Fu-Yu; Wei, Yen; Chiu, Ing-Ming; Hsu, Shan-hui

2015-06-17

An injectable, self-healing hydrogel (≈1.5 kPa) is developed for healing nerve-system deficits. Neurosphere-like progenitors proliferate in the hydrogel and differentiate into neuron-like cells. In the zebrafish injury model, the central nervous system function is partially rescued by injection of the hydrogel and significantly rescued by injection of the neurosphere-laden hydrogel. The self-healing hydrogel may thus potentially repair the central nervous system. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Pathophysiology of Trigger Points in Myofascial Pain Syndrome.

PubMed

Money, Sarah

2017-06-01

Questions from patients about pain conditions and analgesic pharmacotherapy and responses from authors are presented to help educate patients and make them more effective self-advocates. Trigger point pathophysiology in myofascial pain syndrome, which involves muscle stiffness, tenderness, and pain that radiates to other areas of the body, is considered. The causes of trigger points and several theories about how they develop are reviewed, and treatment approaches, including stretching, physical therapy, dry needling, and injections, are offered.

Determination of a Jet Fuel Metal Deactivator by High Performance Liquid Chromatography

DTIC Science & Technology

1983-06-01

bonded phase chromatography (Reference 2). 73 AFWAL-TR-82-2128 Bonded phase packings offer distinct advantages over other packings: a. Irreversible...were then oven dried and placed in a dessicator for cooling and storage until use. The bottles were subsequently silanized with "Glas-TREET" ( Alltech ... advantages of a loop injector are: (1) The volume injected is far more repeatable since a fixed volume loop has a constant volume and is flushed with a

Diesel Fuel Systems. Teacher Edition (Revised).

ERIC Educational Resources Information Center

Clark, Elton; Huston, Jane, Ed.

This module is one of a series of teaching guides that cover diesel mechanics. The module contains six instructional units that cover the following topics: (1) introduction to fuel injection systems and components; (2) injection nozzles; (3) distributor type injection pumps; (4) unit injectors; (5) in-line injection pumps; and (6) pressure timed…

Pulmonary delivery of a GLP-1 receptor agonist, BMS-686117.

PubMed

Qian, Feng; Mathias, Neil; Moench, Paul; Chi, Cecilia; Desikan, Sridhar; Hussain, Munir; Smith, Ronald L

2009-01-21

Alternate delivery route of therapeutic peptides is an attractive non-invasive option to patients who must chronically self-administer their medication through injections. In recent years, much attention has centered on pulmonary peptide delivery of peptide drugs such as insulin and GLP-1 mimetic peptides in the treatment of type II diabetes. In this study, we assessed the feasibility of delivering BMS-686117, an 11-mer GLP-1 receptor peptide agonist, to the lung in rats via intratracheal administration. The pharmacokinetic profiles of three spray-dried, prototype inhaled powder formulations, 80/20 BMS-686117/trehalose (I), 100% BMS-686117 (II), and 20/80 BMS-686117/mannitol (III), as well as a lyophilized BMS-686117 powder, were compared with intravenously and subcutaneously administered peptide. The spray-dried formulations were mostly spherical particles with narrow particle size distribution between 2 to 10 microm, which are better suited for inhalation delivery than the lyophilized, irregular shape powder with a wide particle size distribution between 2 to 100 microm. Prototype III exhibited the best physical characteristics and in vivo performance, with bioavailability of 45% relative to subcutaneous administration. The T(max) for lung delivered peptide formulations were almost twice as fast as subcutaneous injection, suggesting potential for rapid absorption and onset of action. This study demonstrated that pulmonary delivery is a promising, non-invasive route for the administration of BMS-686117.

Oily nanosuspension for long-acting intramuscular delivery of curcumin didecanoate prodrug: preparation, characterization and in vivo evaluation.

PubMed

Wei, Xiao-Lan; Han, Ying-Rui; Quan, Li-Hui; Liu, Chun-Yu; Liao, Yong-Hong

2013-05-13

The objective of this study was to prepare the nanocrystals of curcumin didecanoate (CurDD) by wet ball milling and to investigate the comparative pharmacokinetics of oily nano- and micro-suspensions after intramuscular (i.m.) administration to rats. Upon optimizing the wet ball milling parameters, CurDD nanocrystals were produced with median particle size of ~500 nm and the freeze-dried nanocrystals were readily dispersed in peanut oil to form stable nanosuspensions. Although the nanosuspension appeared to exhibit slower clearance from the injection site after i.m. injection, compared to microsuspension (~5 μm), a significantly higher maximum plasma curcumin concentration (69.0 ng/ml) was observed for the former than that for the latter (18.5 ng/ml). In addition, the nanosuspension provided significant higher plasma curcumin concentrations and brain CurDD contents for at least 15 days than the microsuspension, except for the initial times. A single i.m. injection of nanosuspension appeared to achieve reversal effect on reserpine-induced hypothermia for at least 13 days. This study demonstrates that CurDD nanosuspension may act as a long-acting i.m. injectable for sustained delivery of curcumin, potentially applicable to elicit a long-lasting antidepressant effect. Copyright © 2013 Elsevier B.V. All rights reserved.

Effects of three types of trace element supplementation on the fertility of three commercial dairy herds.

PubMed

Black, D H; French, N P

2004-05-22

The effects on the fertility of three commercial dairy herds of three types of copper- and selenium-containing mineral supplements was investigated. As the cows on each farm were dried off they were allocated to one of three treatment groups, and treated with either subcutaneous injections of copper and selenium, or two matrix intraruminal trace element boluses, or two glass intraruminal trace element boluses. When the data from the 406 cows on the three farms were combined, there was a significant difference between the conception rates of the three groups (P < 0.001). The cows treated with the glass boluses conceived at a rate 1.8 times greater than those treated by injection (P < 0.001), and at a rate 1.5 times greater than those treated with matrix boluses (P = 0.002). These differences were associated with a significantly higher likelihood of service resulting in a conception in the group treated with glass boluses than in the group treated by injection (P = 0.004). After adjusting for time from calving, time from treatment, time of year and farm, there was a significant (P = 0.012) difference in glutathione peroxidase activities between the treatments, with the group treated by injection having a significantly lower activity than the groups treated with boluses.

Liposuction: Anaesthesia challenges

PubMed Central

Sood, Jayashree; Jayaraman, Lakshmi; Sethi, Nitin

2011-01-01

Liposuction is one of the most popular treatment modalities in aesthetic surgery with certain unique anaesthetic considerations. Liposuction is often performed as an office procedure. There are four main types of liposuction techniques based on the volume of infiltration or wetting solution injected, viz dry, wet, superwet, and tumescent technique. The tumescent technique is one of the most common liposuction techniques in which large volumes of dilute local anaesthetic (wetting solution) are injected into the fat to facilitate anaesthesia and decrease blood loss. The amount of lignocaine injected may be very large, approximately 35-55 mg/kg, raising concerns regarding local anaesthetic toxicity. Liposuction can be of two types according to the volume of solution aspirated: High volume (>4,000 ml aspirated) or low volume (<4,000 ml aspirated). While small volume liposuction may be done under local/monitored anaesthesia care, large-volume liposuction requires general anaesthesia. As a large volume of wetting solution is injected into the subcutaneous tissue, the intraoperative fluid management has to be carefully titrated along with haemodynamic monitoring and temperature control. Assessment of blood loss is difficult, as it is mixed with the aspirated fat. Since most obese patients opt for liposuction as a quick method to lose weight, all concerns related to obesity need to be addressed in a preoperative evaluation. PMID:21808392

40 CFR 49.143 - Construction and operational control measures.

Code of Federal Regulations, 2013 CFR

2013-07-01

...: (i) An operating system designed to recover and inject all the produced natural gas into a natural... closed-vent system to: (i) An operating system designed to recover and inject the natural gas emissions... that pipeline injection of all or part of the natural gas collected in an operating system designed to...

40 CFR 49.143 - Construction and operational control measures.

Code of Federal Regulations, 2014 CFR

2014-07-01

...: (i) An operating system designed to recover and inject all the produced natural gas into a natural... closed-vent system to: (i) An operating system designed to recover and inject the natural gas emissions... that pipeline injection of all or part of the natural gas collected in an operating system designed to...

Evaluation of the acute and chronic safety of the biosense injection catheter system in porcine hearts.

PubMed

Kornowski, R; Fuchs, S; Tio, F O; Pierre, A; Epstein, S E; Leon, M B

1999-12-01

Direct myocardial injection of therapeutic agents has been explored as a new method for myocardial revascularization. The integration of a 3D electromechanical mapping catheter with a retractable injection needle should allow for intramyocardial injection to identified sites, obviating the need for open heart surgery. This study assessed the procedural safety and performance characteristics of a novel guided catheter-based transendocardial injection system. The electromagnetic guidance system was coupled with a retrievable 27G needle for left ventricular endocardial injection. Using this system, we injected, transendocardially, methylene-blue (MB) dye tracer at a volume of 0.1 or 0.2 ml per injection in eight normal pigs. Animals were sacrificed acutely, at 1, 3, and 7 days (two animal in each time). Three animals served as controls. The injections were followed by coronary angiography and echocardiogram to assess possible ventricular or coronary perforation and wall motion abnormalities. CK-MB levels were measured up to 24 hr following the procedure. The animals were sacrificed at the assigned time for gross and histopathology evaluation. A total of 101 injections were made in all regions of the heart except the apex and the mitral valve. No animal died as a result of the mapping or injection procedures. Vital signs did not change relative to baseline after the mapping and injection procedures. CK-MB values did not increase over time and there was no evidence of sustained arrhythmia or hemodynamic compromise. There was no evidence of left ventricular or coronary perforation, global or regional wall motion abnormalities, or hemopericardium. On histologic evaluation, the estimated volume of tissue staining was greater than the volume of the injected MB dye due to dispersion of the injectate in the interstitial and intracellular fluid compartments. It is concluded that using this magnetic guidance catheter-based navigational system, it is feasible and safe to perform the transendocardial injection procedure. Thus, if it is determined that direct intramyocardial injection of drugs is a valid therapeutic strategy, this approach offers a clear advantage over surgically based transepicardial injection procedures. Cathet. Cardiovasc. Intervent. 48:447-453, 1999. Copyright 1999 Wiley-Liss, Inc.

Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy

PubMed Central

Zheng, Bei; Li, Wentao; Li, Hongyan; Liu, Lin; Lei, Pei; Ge, Xiaopeng; Yu, Zhiyong; Zhou, Yiqi

2016-01-01

The components for connecting high-performance liquid chromatography (HPLC) with Fourier-transform infrared spectroscopy (FTIR) were investigated to determine estrogen in the water environment, including heating for atomization, solvent removal, sample deposition, drive control, spectrum collection, chip swap, cleaning and drying. Results showed that when the atomization temperature was increased to 388 K, the interference of mobile phase components (methanol, H2O, acetonitrile, and NaH2PO4) were completely removed in the IR measurement of estrogen, with 0.999 of similarity between IR spectra obtained after separation and corresponding to the standard IR spectra. In experiments with varying HPLC injection volumes, high similarity for IR spectra was obtained at 20 ul injection volume at 0.01 mg/L BPA while a useful IR spectrum for 10 ng/L BPA was obtained at 80 ul injection volume. In addition, estrogen concentrations in the natural water samples were calculated semi-quantitatively from the peak intensities of IR spectrum in the mid-infrared region. PMID:27577974

Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy

NASA Astrophysics Data System (ADS)

Zheng, Bei; Li, Wentao; Li, Hongyan; Liu, Lin; Lei, Pei; Ge, Xiaopeng; Yu, Zhiyong; Zhou, Yiqi

2016-08-01

The components for connecting high-performance liquid chromatography (HPLC) with Fourier-transform infrared spectroscopy (FTIR) were investigated to determine estrogen in the water environment, including heating for atomization, solvent removal, sample deposition, drive control, spectrum collection, chip swap, cleaning and drying. Results showed that when the atomization temperature was increased to 388 K, the interference of mobile phase components (methanol, H2O, acetonitrile, and NaH2PO4) were completely removed in the IR measurement of estrogen, with 0.999 of similarity between IR spectra obtained after separation and corresponding to the standard IR spectra. In experiments with varying HPLC injection volumes, high similarity for IR spectra was obtained at 20 ul injection volume at 0.01 mg/L BPA while a useful IR spectrum for 10 ng/L BPA was obtained at 80 ul injection volume. In addition, estrogen concentrations in the natural water samples were calculated semi-quantitatively from the peak intensities of IR spectrum in the mid-infrared region.

Drying kinetics and characteristics of combined infrared-vacuum drying of button mushroom slices

NASA Astrophysics Data System (ADS)

Salehi, Fakhreddin; Kashaninejad, Mahdi; Jafarianlari, Ali

2017-05-01

Infrared-vacuum drying characteristics of button mushroom ( Agaricus bisporus) were evaluated in a combined dryer system. The effects of drying parameters, including infrared radiation power (150-375 W), system pressure (5-15 kPa) and time (0-160 min) on the drying kinetics and characteristics of button mushroom slices were investigated. Both the infrared lamp power and vacuum pressure influenced the drying time of button mushroom slices. The rate constants of the nine different kinetic's models for thin layer drying were established by nonlinear regression analysis of the experimental data which were found to be affected mainly by the infrared power level while system pressure had a little effect on the moisture ratios. The regression results showed that the Page model satisfactorily described the drying behavior of button mushroom slices with highest R value and lowest SE values. The effective moisture diffusivity increases as power increases and range between 0.83 and 2.33 × 10-9 m2/s. The rise in infrared power has a negative effect on the ΔE and with increasing in infrared radiation power it was increased.

Note: Influence of rinsing and drying routines on growth of multilayer thin films using automated deposition system.

PubMed

Gamboa, Daniel; Priolo, Morgan A; Ham, Aaron; Grunlan, Jaime C

2010-03-01

A versatile, high speed robot for layer-by-layer deposition of multifunctional thin films, which integrates concepts from previous dipping systems, has been designed with dramatic improvements in software, positioning, rinsing, drying, and waste removal. This system exploits the electrostatic interaction of oppositely charged species to deposit nanolayers (1-10 nm thick) from water onto the surface of a substrate. Dip times and number of deposited layers are adjustable through a graphical user interface. In between dips the system spray rinses and dries the substrate by positioning it in the two-tiered rinse-dry station. This feature significantly reduces processing time and provides the flexibility to choose from four different procedures for rinsing and drying. Assemblies of natural montmorillonite clay and polyethylenimine are deposited onto 175 microm poly(ethylene terephthalate) film to demonstrate the utility of this automated deposition system. By altering the type of rinse-dry procedure, these clay-based assemblies are shown to exhibit variations in film thickness and oxygen transmission rate. This type of system reproducibly deposits films containing 20 or more layers and may also be useful for other types of coatings that make use of dipping.

Using ground and intact coal Samples to evaluate hydrocarbon fate during supercritical CO2 injection into coal beds: effects of particle size and coal moisture

USGS Publications Warehouse

Kolak, Jon; Hackley, Paul C.; Ruppert, Leslie F.; Warwick, Peter D.; Burruss, Robert

2015-01-01

To investigate the potential for mobilizing organic compounds from coal beds during geologic carbon dioxide (CO2) storage (sequestration), a series of solvent extractions using dichloromethane (DCM) and using supercritical CO2 (40 °C and 10 MPa) were conducted on a set of coal samples collected from Louisiana and Ohio. The coal samples studied range in rank from lignite A to high volatile A bituminous, and were characterized using proximate, ultimate, organic petrography, and sorption isotherm analyses. Sorption isotherm analyses of gaseous CO2 and methane show a general increase in gas storage capacity with coal rank, consistent with findings from previous studies. In the solvent extractions, both dry, ground coal samples and moist, intact core plug samples were used to evaluate effects of variations in particle size and moisture content. Samples were spiked with perdeuterated surrogate compounds prior to extraction, and extracts were analyzed via gas chromatography–mass spectrometry. The DCM extracts generally contained the highest concentrations of organic compounds, indicating the existence of additional hydrocarbons within the coal matrix that were not mobilized during supercritical CO2 extractions. Concentrations of aliphatic and aromatic compounds measured in supercritical CO2 extracts of core plug samples generally are lower than concentrations in corresponding extracts of dry, ground coal samples, due to differences in particle size and moisture content. Changes in the amount of extracted compounds and in surrogate recovery measured during consecutive supercritical CO2extractions of core plug samples appear to reflect the transition from a water-wet to a CO2-wet system. Changes in coal core plug mass during supercritical CO2 extraction range from 3.4% to 14%, indicating that a substantial portion of coal moisture is retained in the low-rank coal samples. Moisture retention within core plug samples, especially in low-rank coals, appears to inhibit accessibility of supercritical CO2 to coal matrix porosity, limiting the extent to which hydrocarbons are mobilized. Conversely, the enhanced recovery of some surrogates from core plugs relative to dry, ground coal samples might indicate that, once mobilized, supercritical CO2 is capable of transporting these constituents through coal beds. These results underscore the need for using intact coal samples, and for better characterization of forms of water in coal, to understand fate and transport of organic compounds during supercritical CO2 injection into coal beds.

Comparison of Selected Metals Content in Cambodian Striped Snakehead Fish (Channa striata) Using Solar Drying System and Open Sun Drying

PubMed Central

Abu Bakar, Nur Faizah; Fudholi, Ahmad; Ruslan, Mohd Hafidz; Saroeun, Im

2015-01-01

The content of 12 elements in Cambodian dried striped snakehead fish was determined using inductively coupled plasma mass spectrometry. The present study compares the level of the trace toxic metals and nutritional trace elements in the fish processed using solar drying system (SDS) and open sun drying (OSD). The skin of SDS fish has lower level of As, Pb, and Cd compared to the OSD sample. As such, the flesh of the fish accumulated higher amount of toxic metals during OSD compared to SDS. However, arsenic was detected in both samples within the safe limit. The nutritional elements (Fe, Mn, Mg, Se, Mo, Cu, Ni, and Cr) were higher in the skin sample SDS fish compared to OSD fish. These beneficial metals were not accumulated in the flesh sample SDS fish demonstrating lower level compared to drying under conventional system. The reddish coloration of the SDS fish was due to the presence of high Cu content in both the skin and flesh samples which possibly account for no mold formation 5 days after packaging. As conclusion, drying of Cambodian C. striata using solar-assisted system has proven higher content of the nutritious elements compared to using the conventional system despite only slight difference in the toxic metals level between the two systems. PMID:25688274

Modelling and simulating the forming of new dry automated lay-up reinforcements for primary structures

NASA Astrophysics Data System (ADS)

Bouquerel, Laure; Moulin, Nicolas; Drapier, Sylvain; Boisse, Philippe; Beraud, Jean-Marc

2017-10-01

While weight has been so far the main driver for the development of prepreg based-composites solutions for aeronautics, a new weight-cost trade-off tends to drive choices for next-generation aircrafts. As a response, Hexcel has designed a new dry reinforcement type for aircraft primary structures, which combines the benefits of automation, out-of-autoclave process cost-effectiveness, and mechanical performances competitive to prepreg solutions: HiTape® is a unidirectional (UD) dry carbon reinforcement with thermoplastic veil on each side designed for aircraft primary structures [1-3]. One privileged process route for HiTape® in high volume automated processes consists in forming initially flat dry reinforcement stacks, before resin infusion [4] or injection. Simulation of the forming step aims at predicting the geometry and mechanical properties of the formed stack (so-called preform) for process optimisation. Extensive work has been carried out on prepreg and dry woven fabrics forming behaviour and simulation, but the interest for dry non-woven reinforcements has emerged more recently. Some work has been achieved on non crimp fabrics but studies on the forming behaviour of UDs are seldom and deal with UD prepregs only. Tension and bending in the fibre direction, along with inter-ply friction have been identified as the main mechanisms controlling the HiTape® response during forming. Bending has been characterised using a modified Peirce's flexometer [5] and inter-ply friction study is under development. Anisotropic hyperelastic constitutive models have been selected to represent the assumed decoupled deformation mechanisms. Model parameters are then identified from associated experimental results. For forming simulation, a continuous approach at the macroscopic scale has been selected first, and simulation is carried out in the Zset framework [6] using proper shell finite elements.

Determination of efavirenz in human dried blood spots by reversed-phase high-performance liquid chromatography with UV detection.

PubMed

Hoffman, Justin T; Rossi, Steven S; Espina-Quinto, Rowena; Letendre, Scott; Capparelli, Edmund V

2013-04-01

Previously published methods for determination of efavirenz (EFV) in human dried blood spots (DBS) use costly and complex liquid chromatography/mass spectrometry. We describe the validation and evaluation of a simple and inexpensive high-performance liquid chromatography method for EFV quantification in human DBS and dried plasma spots (DPS), using ultraviolet detection appropriate for resource-limited settings. One hundred microliters of heparinized whole blood or plasma were spotted onto blood collection cards, dried, punched, and eluted. Eluates are injected onto a C-18 reversed phase high-performance liquid chromatography column. EFV is separated isocratically using a potassium phosphate and acetonitrile mobile phase. Ultraviolet detection is at 245 nm. Quantitation is by use of external calibration standards. Following validation, the method was evaluated using whole blood and plasma from HIV-positive patients undergoing EFV therapy. Mean recovery of drug from DBS is 91.5%. The method is linear over the validated concentration range of 0.3125-20.0 μg/mL. A good correlation (Spearman r = 0.96) between paired plasma and DBS EFV concentrations from the clinical samples was observed, and hematocrit level was not found to be a significant determinant of the EFV DBS level. The mean observed C DBS/C plasma ratio was 0.68. A good correlation (Spearman r = 0.96) between paired plasma and DPS EFV concentrations from the clinical samples was observed. The mean percent deviation of DPS samples from plasma samples is 1.68%. Dried whole blood spot or dried plasma spot sampling is well suited for monitoring EFV therapy in resource-limited settings, particularly when high sensitivity is not essential.

46 CFR 154.1150 - Distribution of dry chemical.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Distribution of dry chemical. 154.1150 Section 154.1150... Firefighting System: Dry Chemical § 154.1150 Distribution of dry chemical. (a) All locations on the above deck... chemical hand hose lines; or (2) At least one dry chemical hand hose line and one dry chemical monitor. (b...

46 CFR 154.1150 - Distribution of dry chemical.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Distribution of dry chemical. 154.1150 Section 154.1150... Firefighting System: Dry Chemical § 154.1150 Distribution of dry chemical. (a) All locations on the above deck... chemical hand hose lines; or (2) At least one dry chemical hand hose line and one dry chemical monitor. (b...

46 CFR 154.1150 - Distribution of dry chemical.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Distribution of dry chemical. 154.1150 Section 154.1150... Firefighting System: Dry Chemical § 154.1150 Distribution of dry chemical. (a) All locations on the above deck... chemical hand hose lines; or (2) At least one dry chemical hand hose line and one dry chemical monitor. (b...

46 CFR 154.1150 - Distribution of dry chemical.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Distribution of dry chemical. 154.1150 Section 154.1150... Firefighting System: Dry Chemical § 154.1150 Distribution of dry chemical. (a) All locations on the above deck... chemical hand hose lines; or (2) At least one dry chemical hand hose line and one dry chemical monitor. (b...

46 CFR 154.1150 - Distribution of dry chemical.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Distribution of dry chemical. 154.1150 Section 154.1150... Firefighting System: Dry Chemical § 154.1150 Distribution of dry chemical. (a) All locations on the above deck... chemical hand hose lines; or (2) At least one dry chemical hand hose line and one dry chemical monitor. (b...

Spray drying of lipid-based systems loaded with Camellia sinensis polyphenols.

PubMed

Secolin, Vanessa A; Souza, Claudia R F; Oliveira, Wanderley P

2017-03-01

In this work, spray-dried lipid systems based on soy phosphatidylcholine, cholesterol and lauroyl polyoxylglycerides for entrapping Green tea polyphenols were produced. The aim was to study the effects of the encapsulating composition and spray drying conditions on the system performance and physicochemical product properties. The spray dryer powder production yield falls around 50.7 ± 2.8%, which is typical for lab scale spray dryers. Wrinkled and rounded particles, with low surface porosities were generated, independent of the drying carriers (trehalose or lactose) used. The product showed high encapsulation efficiency of Green tea polyphenols, which was promptly redispersible in water. It presented low density, and good compressive and flow properties. The results herein reported confirm the feasibility of the entrapment of Green tea polyphenols in lipid-based compositions by spray drying in presence of the drying carriers evaluated. The spray-dried microparticles show high potential to be used as additive in food, nutraceutical and pharmaceutical products.

Deep Stimulation at Newberry Volcano EGS Demonstration

NASA Astrophysics Data System (ADS)

Grasso, K.; Cladouhos, T. T.; Petty, S.; Garrison, G. H.; Nordin, Y.; Uddenberg, M.; Swyer, M.

2014-12-01

The Newberry Volcano EGS Demonstration is a 5 year field project designed to demonstrate recent technological advances for engineered geothermal systems (EGS) development. Advances in reservoir stimulation, diverter, and monitoring are being tested in a hot (>300 C), dry well (NWG 55-29) drilled in 2008. These technologies could reduce the cost of electrical power generation. The project began in 2010 with two years of permitting, technical planning, and development of a project-specific Induced Seismicity Mitigation Plan (ISMP), and is funded in part by the Department of Energy. In 2012, the well was hydraulically stimulated with water at pressures below the principle stress for 7 weeks, resulting in hydroshearing. The depth of stimulation was successfully shifted by injection of two pills of Thermally-degradable Zonal Isolation Materials (TZIMs). Injectivity changes, thermal profiles and seismicity indicate that fracture permeability in well NWG 55-29 was enhanced during stimulation. This work successfully demonstrated the viability of large-volume (40,000 m3), low-pressure stimulation coupled with non-mechanical diverter technology, and microseismic monitoring for reservoir mapping. Further analysis and field testing in 2013 indicates further stimulation will be required in order to develop an economically viable reservoir, and is scheduled in 2014. The 2014 stimulation will use improved stimulation and monitoring equipment, better knowledge based on 2012 outcomes, and create a deep EGS reservoir in the hottest part of the wellbore.

Arabidopsis-derived shrimp viral-binding protein, PmRab7 can protect white spot syndrome virus infection in shrimp.

PubMed

Thagun, Chonprakun; Srisala, Jiraporn; Sritunyalucksana, Kallaya; Narangajavana, Jarunya; Sojikul, Punchapat

2012-09-15

White spot syndrome virus is currently the leading cause of production losses in the shrimp industry. Penaeus monodon Rab7 protein has been recognized as a viral-binding protein with an efficient protective effect against white spot syndrome infection. Plant-derived recombinant PmRab7 might serve as an alternative source for in-feed vaccination, considering the remarkable abilities of plant expression systems. PmRab7 was introduced into the Arabidopsis thaliana T87 genome. Arabidopsis-derived recombinant PmRab7 showed high binding activity against white spot syndrome virus and a viral envelope, VP28. The growth profile of Arabidopsis suspension culture expressing PmRab7 (ECR21# 35) resembled that of its counterpart. PmRab7 expression in ECR21# 35 reached its maximum level at 5 mg g(-1) dry weight in 12 days, which was higher than those previously reported in Escherichia coli and in Pichia. Co-injection of white spot syndrome virus and Arabidopsis crude extract containing PmRab7 in Litopenaeus vannamei showed an 87% increase in shrimp survival rate at 5 day after injection. In this study, we propose an alternative PmRab7 source with higher production yield, and cheaper culture media costs, that might serve the industry's need for an in-feed supplement against white spot syndrome infection. Copyright © 2012 Elsevier B.V. All rights reserved.

Experimental investigation of geochemical and mineralogical effects of CO2 sequestration on flow characteristics of reservoir rock in deep saline aquifers

PubMed Central

Rathnaweera, T. D.; Ranjith, P. G.; Perera, M. S. A.

2016-01-01

Interactions between injected CO2, brine, and rock during CO2 sequestration in deep saline aquifers alter their natural hydro-mechanical properties, affecting the safety, and efficiency of the sequestration process. This study aims to identify such interaction-induced mineralogical changes in aquifers, and in particular their impact on the reservoir rock’s flow characteristics. Sandstone samples were first exposed for 1.5 years to a mixture of brine and super-critical CO2 (scCO2), then tested to determine their altered geochemical and mineralogical properties. Changes caused uniquely by CO2 were identified by comparison with samples exposed over a similar period to either plain brine or brine saturated with N2. The results show that long-term reaction with CO2 causes a significant pH drop in the saline pore fluid, clearly due to carbonic acid (as dissolved CO2) in the brine. Free H+ ions released into the pore fluid alter the mineralogical structure of the rock formation, through the dissolution of minerals such as calcite, siderite, barite, and quartz. Long-term CO2 injection also creates a significant CO2 drying-out effect and crystals of salt (NaCl) precipitate in the system, further changing the pore structure. Such mineralogical alterations significantly affect the saline aquifer’s permeability, with important practical consequences for the sequestration process. PMID:26785912

Cotton gin drying systems–patterns in fuel energy use

USDA-ARS?s Scientific Manuscript database

Fuel cost increases and consumption variability threaten gin profitability. Twenty-three U.S. cotton gins were audited to elucidate drying system components, layouts and process control strategies that made better use of fuel. Seed cotton samples were obtained before and after each drying system a...

Expression of SIRT1 and oxidative stress in diabetic dry eye.

PubMed

Liu, Hao; Sheng, Minjie; Liu, Yu; Wang, Peng; Chen, Yihui; Chen, Li; Wang, Weifang; Li, Bing

2015-01-01

To explore the expression of SIRT1 with oxidative stress and observe physiological and pathological changes in the corneas as well as the association between SIRT1 and oxidative stress of diabetic dry eyes in mice. Forty-eight C57BL/6Jdb/db mice at eight weeks of age were divided randomly into two groups: the diabetic dry eye group and the diabetic group. An additional forty-eight C57BL/6J mice at eight weeks of age were divided randomly into two groups: the dry eye group and the control group. Every mouse in the dry eye groups (diabetic and normal) was injected with scopolamine hydrobromide three times daily, combined with low humidity to establish a dry eye model. After the intervention, phenol red cotton string tests and corneal fluorescein staining were performed. In addition, HE staining and immunofluorescence were done. Expression of SIRT1 in the cornea was examined by real-time PCR and Western Blot and expression of FOXO3 and MnSOD proteins was detected by Western Blot. At one, four, and eight weeks post intervention, all of the groups except the controls showed significant decreases in tear production and increases in the corneal fluorescein stain (P<0.05 vs control). Between the experimental groups, the diabetic dry eye group had the least tear production and the highest corneal fluorescein stain score (P<0.05). As the disease progressed, all of the experimental groups showed obviously pathological changes in HE staining, particularly the diabetic dry eye group. In the 1(st) and 4(th) week, the expression of SIRT1, FOXO3, and MnSOD were significantly higher in the diabetic DE and DM groups but lower in the DE group compared to the controls (P<0.05). In the 8(th) week, the expression of SIRT1, FOXO3, and MnSOD was significantly down-regulated in the diabetic DE group and the DM group (P<0.05). Immunofluorescence showed similar results. In the condition of diabetic dry eye, tear production declined markedly coupled with seriously wounded corneal epithelium. Oxidative stress in the cornea was enhanced significantly and the expression of SIRT1 was decreased.

Active waste-injection systems in Florida, 1976

USGS Publications Warehouse

Vecchioli, John; McKenzie, D.J.; Pascale, C.A.; Wilson, W.E.

1979-01-01

As of the end of 1976, seven systems were injecting liquid wastes into Florida 's subsurface environment at a combined average rate of 15 million gallons per day. This report presents for each of these systems information on the kind and amount of waste injected and type of pretreatment, construction characteristics of the injection and monitor wells, type of test and monitoring data available, and brief discussion of any operational problems experienced. (Kosco-USGS)

[Microinjection Monitoring System Design Applied to MRI Scanning].

PubMed

Xu, Yongfeng

2017-09-30

A microinjection monitoring system applied to the MRI scanning was introduced. The micro camera probe was used to stretch into the main magnet for real-time video injection monitoring of injection tube terminal. The programming based on LabVIEW was created to analysis and process the real-time video information. The feedback signal was used for intelligent controlling of the modified injection pump. The real-time monitoring system can make the best use of injection under the condition that the injection device was away from the sample which inside the magnetic room and unvisible. 9.4 T MRI scanning experiment showed that the system in ultra-high field can work stability and doesn't affect the MRI scans.

Particulate-matter content of 11 cephalosporin injections: conformance with USP limits.

PubMed

Parkins, D A; Taylor, A J

1987-05-01

The particulate-matter content of 11 dry-powder cephalosporin injections was determined using a modified version of the official United States Pharmacopeial Convention (USP) method for particulate matter in small-volume injections (SVIs). Ten vials of each cephalosporin product were each constituted with 10 mL of Water for Injections BP that had been filtered through a 0.22-micron membrane. The pooled contents of the 10 vials for each product were allowed to stand under reduced pressure to ensure removal of gas bubbles. Particulate-matter content was determined using a HIAC/Royco particle counter on six 10-mL samples obtained from the pooled solutions for each product. All solution preparation and particle counting was performed in a horizontal-laminar-airflow hood. Modifications of the USP method used in this study included the use of six rather than two samples from each pooled solution, the addition of diluent to the injections through the rubber closure with a needle instead of into the open container, and changes in the degassing method. Particle counts for all products examined were lower than USP limits for SVIs. All but two products contained less than 15% of USP limits for particles greater than or equal to 10 microns in effective diameter and particles greater than or equal to 25 microns in effective diameter. The standard USP method for degassing (standing for two minutes) was inadequate. Application of reduced pressure for up to 10 minutes was necessary for thorough degassing of products.(ABSTRACT TRUNCATED AT 250 WORDS)

Aflibercept in refractory wet AMD treated with ranibizumab: Anatomical and visual outcome.

PubMed

Abou-Ltaif, Sleiman

2016-01-01

To assess the anatomical outcome of patients with exudative age-related macular degeneration resistant to ranibizumab treated with aflibercept. Prospective, interventional, case series, where we treated a group of patients deemed resistant to Ranibizumab after 6 months of persistence of intra- or subretinal fluid despite continuous treatment. The study included 17 patients, 3 males and 14 females. The average males age was 85 (range 83-87), and that of females was 79.64 (range 68-88). At the start of the study, the central foveal thickness CFT average was 534.76 μm (range 252-999). At 1 month and after 1 injection of Aflibercept, the CFT average was 324.82 μm (range 222-585). At 4 months and after 3 consecutive injections of Aflibercept the CFT average was 294.76 μm (range 184-640). At 6 months the CFT average was 356 μm (range 206-609). At the 5th visit only 8 out 17 (47%) patients required repeated injection either for persistent fluids or for recurrence. At the 6th and final visit only 4 out of 17 (23.5%) needed repeated injections, of them only one was treated on the visit before and treatment was given as very little response was observed from last injection, and all other 3 were not treated on the visit before. Our results showed that aflibercept was able to dry the macula even in advanced case of wet AMD resistant to Ranibizumab.

Designing Fault-Injection Experiments for the Reliability of Embedded Systems

NASA Technical Reports Server (NTRS)

White, Allan L.

2012-01-01

This paper considers the long-standing problem of conducting fault-injections experiments to establish the ultra-reliability of embedded systems. There have been extensive efforts in fault injection, and this paper offers a partial summary of the efforts, but these previous efforts have focused on realism and efficiency. Fault injections have been used to examine diagnostics and to test algorithms, but the literature does not contain any framework that says how to conduct fault-injection experiments to establish ultra-reliability. A solution to this problem integrates field-data, arguments-from-design, and fault-injection into a seamless whole. The solution in this paper is to derive a model reduction theorem for a class of semi-Markov models suitable for describing ultra-reliable embedded systems. The derivation shows that a tight upper bound on the probability of system failure can be obtained using only the means of system-recovery times, thus reducing the experimental effort to estimating a reasonable number of easily-observed parameters. The paper includes an example of a system subject to both permanent and transient faults. There is a discussion of integrating fault-injection with field-data and arguments-from-design.

Image-guided intraocular injection using multimodality optical coherence tomography and fluorescence confocal scanning laser ophthalmoscopy in rodent ophthalmological models

NASA Astrophysics Data System (ADS)

Terrones, Benjamin D.; Benavides, Oscar R.; Leeburg, Kelsey C.; Mehanathan, Sankarathi B.; Levine, Edward M.; Tao, Yuankai K.

2018-02-01

Intraocular injections are routinely performed for delivery of anti-VEGF and anti-inflammatory therapies in humans. While these injections are also performed in mice to develop novel models of ophthalmic diseases and screen novel therapeutics, the injection location and volume are not well-controlled and reproducible. We overcome limitations of conventional injections methods by developing a multimodality, long working distance, non-contact optical coherence tomography (OCT) and fluorescence confocal scanning laser ophthalmoscopy (cSLO) system for retinal imaging before and after injections. Our OCT+cSLO system combines a custom-built spectraldomain OCT engine (875+/-85 nm) with 125 kHz line-rate with a modified commercial cSLO with a maximum frame-rate of 30 fps (512 x 512 pix.). The system was designed for an overlapping OCT+cSLO field-of-view of 1.1 mm with a 7.76 mm working distance to the pupil. cSLO excitation light sources and filters were optimized for simultaneous GFP and tdTomato imaging. Lateral resolution was 3.02 µm for OCT and 2.74 μm for cSLO. Intravitreal injections of 5%, 10%, and 20% intralipid with Alex Fluor 488 were manually injected intraocularly in C57BL/6 mice. Post-injection imaging showed structural changes associated with retinal puncture, including the injection track, a retinal elevation, and detachment of the posterior hyaloid. OCT enables quantitative analysis of injection location and volumes whereas complementary cSLO improves specificity for identifying fluorescently labeled injected compounds and transgenic cells. The long working distance of our non-contact OCT+cSLO system is uniquely-suited for concurrent imaging with intraocular injections and may be applied for imaging of ophthalmic surgical dynamics and real-time image-guided injections.

Challenges and Plans for Injection and Beam Dump

NASA Astrophysics Data System (ADS)

Barnes, M.; Goddard, B.; Mertens, V.; Uythoven, J.

The injection and beam dumping systems of the LHC will need to be upgraded to comply with the requirements of operation with the HL-LHC beams. The elements of the injection system concerned are the fixed and movable absorbers which protect the LHC in case of an injection kicker error and the injection kickers themselves. The beam dumping system elements under study are the absorbers which protect the aperture in case of an asynchronous beam dump and the beam absorber block. The operational limits of these elements and the new developments in the context of the HL-LHC project are described.

Incipient flocculation molding: A new ceramic-forming technique

NASA Astrophysics Data System (ADS)

Arrasmith, Steven Reade

Incipient Flocculation Molding (IFM) was conceived as a new near-net-shape forming technique for ceramic components. It was hypothesized that the development of a temperature-dependent deflocculant would result in a forming technique that is flexible, efficient, and capable of producing a superior microstructure with improved mechanical properties from highly reactive, submicron ceramic powders. IFM utilizes a concentrated, nonaqueous, sterically stabilized ceramic powder and/or colloidal suspension which is injected into a non-porous mold. The suspension is then flocculated by destabilizing the suspension by lowering the temperature. Flocculation is both rapid and reversible. Cooling to -20°C produces a green body with sufficient strength for removal from the mold. The solvent is removed from the green body by evaporation. The dried green body is subsequently sintered to form a dense ceramic monolith. This is the first ceramic forming method based upon the manipulation of a sterically-stabilized suspension. To demonstrate IFM, the process of grafting polyethylene glycol (PEG), with molecular weights from 600 to 8000, to alumina powders was investigated. The maximum grafted amounts were achieved by the technique of dispersing the alumina powders in molten polymer at 195°C. The ungrafted PEG was then removed by repeated centrifuging and redispersion in fresh distilled water. The rheological behavior of suspensions of the PEG-grafted powders in water, 2-propanol and 2-butanol were characterized. All of the aqueous suspensions were shear thinning. The PEG 4600-grafted alumina powder aqueous suspensions were the most fluid. Sample rods and bars were molded from 52 vol% PEG-grafted alumina suspensions in 2-butanol. The best results were obtained with a preheated aluminum mold lubricated with a fluorinated oil mold-release. The samples were dried, sintered, and their microstructure and density were compared with sintered samples dry pressed from the same alumina powder. Densities and microstructures were quite similar to those obtained by dry pressing and sintering these powders. Dried green samples with densities of ca. 57% of theoretical sintered to >96% of theoretical density. This research has demonstrated IFM as a viable ceramic forming process which has potential to be developed into an industrial process. Further research is needed to determine preferred molding parameters, other possible polymer-solvent systems, and investigate the use of other ceramic powders. The concepts developed for IFM may have potential applications in other ceramic forming processes, such as extrusion and rapid prototyping.

Evaluation of performance, safety, subject acceptance, and compliance of a disposable autoinjector for subcutaneous injections in healthy volunteers.

PubMed

Berteau, Cecile; Schwarzenbach, Florence; Donazzolo, Yves; Latreille, Mathilde; Berube, Julie; Abry, Herve; Cotten, Joël; Feger, Celine; Laurent, Philippe E

2010-10-05

A disposable autoinjector was developed for subcutaneous (SC) self-injection by patients with chronic diseases. To verify its performance and evaluate its acceptance, a clinical study was conducted in healthy volunteers, comparing SC injections performed by subjects using the autoinjector with SC injections performed by nurses using a syringe. This was a randomized, single-center, crossover study comparing SC self-injection using an autoinjector with SC nurse-administered injection using a syringe. Two volumes (0.2 mL and 1 mL) were injected into healthy volunteers. Study objectives included assessment of the accuracy and consistency of the volume injected by the injection systems, and skin reaction and pain associated with the injection. The fluid depot in the SC tissue layer was evaluated by ultrasound. Subject acceptance was evaluated using questionnaires on attitudes and emotions towards the injection technique, and challenged by seeking the subjects' preferred system for a final study injection or future treatment. A total of 960 injections (480 with autoinjector, 480 with syringe) were performed in 40 subjects. There were no significant differences in mean fluid leakage and injected volumes between the systems. Pain associated with the injection was significantly lower with the auto-injector than with the syringe. Local skin reaction at the injection site was overall satisfactory. Injections were appropriately performed by all subjects. At study end, all 40 subjects preferred the autoinjector for a final study injection and for future treatment. This study indicated that the autoinjector used by the subject was similar to a syringe used by a nurse in terms of performance and safety in administering the injections, and better in terms of pain, overall acceptance, and preference.

Development of a real-time chemical injection system for air-assisted variable-rate sprayers

USDA-ARS?s Scientific Manuscript database

A chemical injection system is an effective method to minimize chemical waste and reduce the environmental pollution in pesticide spray applications. A microprocessor controlled injection system implementing a ceramic piston metering pump was developed to accurately dispense chemicals to be mixed wi...

Installation, maintenance and operating manual for the Lucas-type fuel injection system of the 3 B rotary engine

NASA Technical Reports Server (NTRS)

1985-01-01

The installation procedure, maintenance, adjustment and operation of a Lucas type fuel injection system for 13B rotary racing engine is outlined. Components of the fuel injection system and installation procedure and notes are described. Maintenance, adjustment, and operation are discussed.

Variable volume combustor with pre-nozzle fuel injection system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Keener, Christopher Paul; Johnson, Thomas Edward; McConnaughhay, Johnie Franklin

The present application provides a combustor for use with a gas turbine engine. The combustor may include a number of fuel nozzles, a pre-nozzle fuel injection system supporting the fuel nozzles, and a linear actuator to maneuver the fuel nozzles and the pre-nozzle fuel injection system.

Penetration and Duration of Fuel Sprays from a Pump Injection System

NASA Technical Reports Server (NTRS)

Rothrock, A M; Marsh, E T

1934-01-01

High-speed motion pictures were taken of individual fuel sprays from a pump injection system. The changes in the spray-tip penetration with changes in the pump speed, injection-valve opening and closing pressures, discharge-orifice area, injection-tube length and diameter, and pump throttle setting were measured. The pump was used with and without a check valve. The results show that the penetration of the spray tip can be controlled by the dimensions of the injection tube, the area of the discharge orifice, and the injection-valve opening and closing pressures.

Exergy analysis of encapsulation of photochromic dye by spray drying

NASA Astrophysics Data System (ADS)

Çay, A.; Akçakoca Kumbasar, E. P.; Morsunbul, S.

2017-10-01

Application of exergy analysis methodology for encapsulation of photochromic dyes by spray drying was presented. Spray drying system was investigated considering two subsystems, the heater and the dryer sections. Exergy models for each subsystem were proposed and exergy destruction rate and exergy efficiency of each subsystem and the whole system were computed. Energy and exergy efficiency of the system were calculated to be 5.28% and 3.40%, respectively. It was found that 90% of the total exergy inlet was destroyed during encapsulation by spray drying and the exergy destruction of the heater was found to be higher.

Engine Company Evaluation of Feasibility of Aircraft Retrofit Water-Injected Turbomachines

NASA Technical Reports Server (NTRS)

Becker, Arthur

2006-01-01

This study supports the NASA Glenn Research Center and the U.S. Air Force Research Laboratory in their efforts to evaluate the effect of water injection on aircraft engine performance and emissions. In this study, water is only injected during the takeoff and initial climb phase of a flight. There is no water injection during engine start or ground operations, nor during climb, cruise, descent, or landing. This study determined the maintenance benefit of water injection during takeoff and initial climb and evaluated the feasibility of retrofitting a current production engine, the PW4062 (Pratt & Whitney, East Hartford, CT), with a water injection system. Predicted NO(x) emissions based on a 1:1 water-tofuel ratio are likely to be reduced between 30 to 60 percent in Environmental Protection Agency parameter (EPAP). The maintenance cost benefit for an idealized combustor water injection system installed on a PW4062 engine in a Boeing 747-400ER aircraft (The Boeing Company, Chicago, IL) is computed to be $22 per engine flight hour (EFH). Adding water injection as a retrofit kit would cost up to $375,000 per engine because of the required modifications to the fuel system and addition of the water supply system. There would also be significant nonrecurring costs associated with the development and certification of the system that may drive the system price beyond affordability.

Fertilizer from dried human urine added to ash and lime - a potential product from eco-sanitation system.

PubMed

Dutta, Shanta; Vinnerås, Björn

2016-09-01

This research explored the possibility of making fertilizer at a laboratory from source separated and untreated human urine added to ash and lime by drying at low temperatures. A mixture of ash and lime (1:1) was used as drying agent and human urine was applied as undiluted and fresh. Ash and lime were chosen as drying agents for maintaining a pH > 10 during the drying process, which should inhibit urea hydrolysis in urine, and thereby urea should be retained in the drying agent. The drying technique was developed and drying capacity of the system was quantified; three specific temperatures (20 °, 35 °, 60 °C) and two airflow rates (1 L/min and 5 L/min) were used in the experiment. A mass balance for nitrogen in the system was obtained. It was evident from the experiment that urea can be retained by maintaining a high pH (>10). Urine drying at 20 °C was not a feasible option, since rate of evaporation was very low. The highest retention of inflow nitrogen at 35 °C and 60 °C were 74% and 54%, respectively, in the produced fertilizer. Reduced evaporation rate, flooding of urine over drying agent, and blockage in airflow influenced nitrogen loss and concentration of nitrogen in the final product.

Research on the technology of detecting the SQL injection attack and non-intrusive prevention in WEB system

NASA Astrophysics Data System (ADS)

Hu, Haibin

2017-05-01

Among numerous WEB security issues, SQL injection is the most notable and dangerous. In this study, characteristics and procedures of SQL injection are analyzed, and the method for detecting the SQL injection attack is illustrated. The defense resistance and remedy model of SQL injection attack is established from the perspective of non-intrusive SQL injection attack and defense. Moreover, the ability of resisting the SQL injection attack of the server has been comprehensively improved through the security strategies on operation system, IIS and database, etc.. Corresponding codes are realized. The method is well applied in the actual projects.

Manufacturing Solid Dosage Forms from Bulk Liquids Using the Fluid-bed Drying Technology.

PubMed

Qi, Jianping; Lu, Y I; Wu, Wei

2015-01-01

Solid dosage forms are better than liquid dosage forms in many ways, such as improved physical and chemical stability, ease of storage and transportation, improved handling properties, and patient compliance. Therefore, it is required to transform dosage forms of liquid origins into solid dosage forms. The functional approaches are to absorb the liquids by solid excipients or through drying. The conventional drying technologies for this purpose include drying by heating, vacuum-, freeze- and spray-drying, etc. Among these drying technologies, fluidbed drying emerges as a new technology that possesses unique advantages. Fluid-bed drying or coating is highly efficient in solvent removal, can be performed at relatively low temperatures, and is a one-step process to manufacture formulations in pellet forms. In this article, the status of the art of manufacturing solid dosage forms from bulk liquids by fluid-bed drying technology was reviewed emphasizing on its application in solid dispersion, inclusion complexes, self-microemulsifying systems, and various nanoscale drug delivery systems.

Design of a Coupled Thermoresponsive Hydrogel and Robotic System for Postinfarct Biomaterial Injection Therapy

PubMed Central

Fok, Kevin; Yoshizumi, Tomo; Park, Daewoo; Jiang, Hongbin; Schwartzman, David S.; Zenati, Marco A.; Uchibori, Takafumi

2016-01-01

Background In preclinical testing, ventricular wall injection of hydrogels has been shown to be effective in modulating ventricular remodeling and preserving cardiac function. For some approaches, early-stage clinical trials are under way. The hydrogel delivery method varies, with minimally invasive approaches being preferred. Endocardial injections carry a risk of hydrogel regurgitation into the circulation, and precise injection patterning is a challenge. An epicardial approach with a thermally gelling hydrogel through the subxiphoid pathway overcomes these disadvantages. Methods A relatively stiff, thermally responsive, injectable hydrogel based on N-isopropylacrylamide and N-vinylpyrrolidone (VP gel) was synthesized and characterized. VP gel thermal behavior was tuned to couple with a transepicardial injection robot, incorporating a cooling feature to achieve injectability. Ventricular wall injections of the optimized VP gel have been performed ex vivo and on beating porcine hearts. Results Thermal transition temperature, viscosity, and gelling time for the VP gel were manipulated by altering N-vinylpyrrolidone content. The target parameters for cooling in the robotic system were chosen by thermal modeling to support smooth, repeated injections on an ex vivo heart. Injections at predefined locations and depth were confirmed in an infarcted porcine model. Conclusions A coupled thermoresponsive hydrogel and robotic injection system incorporating a temperature-controlled injectate line was capable of targeted injections and amenable to use with a subxiphoid transepicardial approach for hydrogel injection after myocardial infarction. The confirmation of precise location and depth injections would facilitate a patient-specific planning strategy to optimize injection patterning to maximize the mechanical benefits of hydrogel placement. PMID:27154150

Effects of Direct Renin Blockade on Renal & Systemic Hemodynamics and on RAAS Activity, in Weight Excess and Hypertension: A Randomized Clinical Trial

PubMed Central

Kwakernaak, A. J.; Roksnoer, L. C.; Lambers Heerspink, H. J.; van den Berg-Garrelds, I.; Lochorn, G. A.; van Embden Andres, J. H.; Klijn, M. A.; Kobori, H.; Danser, A. H. J.; Laverman, G. D.; Navis, G. J.

2017-01-01

Aim The combination of weight excess and hypertension significantly contributes to cardiovascular risk and progressive kidney damage. An unfavorable renal hemodynamic profile is thought to contribute to this increased risk and may be ameliorated by direct renin inhibition (DRI). The aim of this trial was to assess the effect of DRI on renal and systemic hemodynamics and on RAAS activity, in men with weight excess and hypertension. Methods A randomized, double-blind, cross-over clinical trial to determine the effect of DRI (aliskiren 300 mg/day), with angiotensin converting enzyme inhibition (ACEi; ramipril 10 mg/day) as a positive control, on renal and systemic hemodynamics, and on RAAS activity (n = 15). Results Mean (SEM) Glomerular filtration rate (101 (5) mL/min/1.73m2) remained unaffected by DRI or ACEi. Effective renal plasma flow (ERPF; 301 (14) mL/min/1.73m2) was increased in response to DRI (320 (14) mL/min/1.73m2, P = 0.012) and ACEi (317 (15) mL/min/1.73m2, P = 0.045). Filtration fraction (FF; 34 (0.8)%) was reduced by DRI only (32 (0.7)%, P = 0.044). Mean arterial pressure (109 (2) mmHg) was reduced by DRI (101 (2) mmHg, P = 0.008) and ACEi (103 (3) mmHg, P = 0.037). RAAS activity was reduced by DRI and ACEi. Albuminuria (20 [9–42] mg/d) was reduced by DRI only (12 [5–28] mg/d, P = 0.030). Conclusions In men with weight excess and hypertension, DRI and ACEi improved renal and systemic hemodynamics. Both DRI and ACEi reduced RAAS activity. Thus, DRI provides effective treatment in weight excess and hypertension. Trial Registration Dutch trial register, registration number: 2532 www.trialregister.nl PMID:28118402

Polymeric microchip for the simultaneous determination of anions and cations by hydrodynamic injection using a dual-channel sequential injection microchip electrophoresis system.

PubMed

Gaudry, Adam J; Nai, Yi Heng; Guijt, Rosanne M; Breadmore, Michael C

2014-04-01

A dual-channel sequential injection microchip capillary electrophoresis system with pressure-driven injection is demonstrated for simultaneous separations of anions and cations from a single sample. The poly(methyl methacrylate) (PMMA) microchips feature integral in-plane contactless conductivity detection electrodes. A novel, hydrodynamic "split-injection" method utilizes background electrolyte (BGE) sheathing to gate the sample flows, while control over the injection volume is achieved by balancing hydrodynamic resistances using external hydrodynamic resistors. Injection is realized by a unique flow-through interface, allowing for automated, continuous sampling for sequential injection analysis by microchip electrophoresis. The developed system was very robust, with individual microchips used for up to 2000 analyses with lifetimes limited by irreversible blockages of the microchannels. The unique dual-channel geometry was demonstrated by the simultaneous separation of three cations and three anions in individual microchannels in under 40 s with limits of detection (LODs) ranging from 1.5 to 24 μM. From a series of 100 sequential injections the %RSDs were determined for every fifth run, resulting in %RSDs for migration times that ranged from 0.3 to 0.7 (n = 20) and 2.3 to 4.5 for peak area (n = 20). This system offers low LODs and a high degree of reproducibility and robustness while the hydrodynamic injection eliminates electrokinetic bias during injection, making it attractive for a wide range of rapid, sensitive, and quantitative online analytical applications.

On finding the analytic dependencies of the external field potential on the control function when optimizing the beam dynamics

NASA Astrophysics Data System (ADS)

Ovsyannikov, A. D.; Kozynchenko, S. A.; Kozynchenko, V. A.

2017-12-01

When developing a particle accelerator for generating the high-precision beams, the injection system design is of importance, because it largely determines the output characteristics of the beam. At the present paper we consider the injection systems consisting of electrodes with given potentials. The design of such systems requires carrying out simulation of beam dynamics in the electrostatic fields. For external field simulation we use the new approach, proposed by A.D. Ovsyannikov, which is based on analytical approximations, or finite difference method, taking into account the real geometry of the injection system. The software designed for solving the problems of beam dynamics simulation and optimization in the injection system for non-relativistic beams has been developed. Both beam dynamics and electric field simulations in the injection system which use analytical approach and finite difference method have been made and the results presented in this paper.

Steroid intracochlear distribution differs by administration method: Systemic versus intratympanic injection.

PubMed

Lee, Jong Joo; Jang, Jeong Hun; Choo, Oak-Sung; Lim, Hye Jin; Choung, Yun-Hoon

2018-01-01

Steroids have been widely used to treat inner-ear diseases such as sudden sensorineural hearing loss, tinnitus, and Meniere's disease. They can be given via either systemic or intratympanic (IT) injection. The purpose of the present study was to explore differences in intracochlear steroid distribution by the administration method employed (systemic vs. IT injection). Animal study. Twenty-three Sprague-Dawley rats were given fluorescein isothiocyanate-labeled dexamethasone (FITC-DEX) three times (on successive days) via intraperitoneal (IP) or IT injection. Cochlear uptake of FITC-DEX was evaluated via immunohistochemistry and flow cytometry at 6 hours, and 3 and 7 days after the final injection. FITC-DEX uptake was evident in spiral ganglion cells (SGs), the organ of Corti (OC), and the lateral walls (LWs), the basal turns of which were stained relatively prominently in both groups. Animals receiving IP injections exhibited higher FITC-DEX uptakes by the SGs and OC, whereas IT injection triggered higher-level FITC-DEX accumulation by the OC and LWs. Flow cytometry revealed that intracochlear FITC-DEX uptake by IT-injected animals was higher and more prolonged than in animals subjected to IP injections. We thus describe differences in cochlear steroid distributions after systemic and IT injections. This finding could help our understanding of the pharmacokinetics of steroids in the cochlea. NA. Laryngoscope, 128:189-194, 2018. © 2017 The American Laryngological, Rhinological and Otological Society, Inc.

A semi-automated vascular access system for preclinical models

NASA Astrophysics Data System (ADS)

Berry-Pusey, B. N.; Chang, Y. C.; Prince, S. W.; Chu, K.; David, J.; Taschereau, R.; Silverman, R. W.; Williams, D.; Ladno, W.; Stout, D.; Tsao, T. C.; Chatziioannou, A.

2013-08-01

Murine models are used extensively in biological and translational research. For many of these studies it is necessary to access the vasculature for the injection of biologically active agents. Among the possible methods for accessing the mouse vasculature, tail vein injections are a routine but critical step for many experimental protocols. To perform successful tail vein injections, a high skill set and experience is required, leaving most scientists ill-suited to perform this task. This can lead to a high variability between injections, which can impact experimental results. To allow more scientists to perform tail vein injections and to decrease the variability between injections, a vascular access system (VAS) that semi-automatically inserts a needle into the tail vein of a mouse was developed. The VAS uses near infrared light, image processing techniques, computer controlled motors, and a pressure feedback system to insert the needle and to validate its proper placement within the vein. The VAS was tested by injecting a commonly used radiolabeled probe (FDG) into the tail veins of five mice. These mice were then imaged using micro-positron emission tomography to measure the percentage of the injected probe remaining in the tail. These studies showed that, on average, the VAS leaves 3.4% of the injected probe in the tail. With these preliminary results, the VAS system demonstrates the potential for improving the accuracy of tail vein injections in mice.

Effect of the Reservoir Volume on the Discharge Pressures in the Injection System of the N.A.C.A. Spray Photography Equipment

NASA Technical Reports Server (NTRS)

Rothrock, A M; Lee, D W

1932-01-01

Tests were made to determine the effect of the reservoir volume on the discharge pressures in the injection system of the N.A.C.A. spray photography equipment. The data obtained are applicable to the design of a common rail fuel-injection system. The data show that an injection system of the type described can be designed so that not more than full load fuel quantity can be injected into the engine cylinders, and so that the fuel spray characteristics remain constant over a large range of engine speeds. Formulas are presented for computing the volume of the reservoir and the diameter of the discharge orifice.

Injection-depth-locking axial motion guided handheld micro-injector using CP-SSOCT.

PubMed

Cheon, Gyeong Woo; Huang, Yong; Kwag, Hye Rin; Kim, Ki-Young; Taylor, Russell H; Gehlbach, Peter L; Kang, Jin U

2014-01-01

This paper presents a handheld micro-injector system using common-path swept source optical coherence tomography (CP-SSOCT) as a distal sensor with highly accurate injection-depth-locking. To achieve real-time, highly precise, and intuitive freehand control, the system used graphics processing unit (GPU) to process the oversampled OCT signal with high throughput and a smart customized motion monitoring control algorithm. A performance evaluation was conducted with 60-insertions and fluorescein dye injection tests to show how accurately the system can guide the needle and lock to the target depth. The evaluation tests show our system can guide the injection needle into the desired depth with 4.12 um average deviation error while injecting 50 nl of fluorescein dye.

Assessment of the Capability of the NGDS Prototype to Replace the JBAIDS for Environmental Sample Analysis

DTIC Science & Technology

2016-11-01

FilmArray system. 15. SUBJECT TERMS BioFire FilmArray system Dry Filter Unit (DFU) Joint Biological...collectors, such as the Dry Filter Unit (DFU). The FilmArray system showed enormous potential to rapidly screen samples for a wide range of biological...specific focus on the efficient implementation of environmental detectors and collectors, such as Dry Filter Units (DFUs). The JUPITR ATD members

Development of a nanosuspension for iv administration: From miniscale screening to a freeze dried formulation.

PubMed

Frank, Kerstin J; Boeck, Georg

2016-05-25

The aim was to develop a nanosuspension of the poorly soluble BI XX. The nanosuspension is intended for intravenous (iv) administration in preclinical studies and should not cause any unwanted side effects. Thus, only stabilizers that are accepted for iv application should be used and isotonicity, euhydria and the absence of living microorganisms were targeted. Suspensions were prepared in a ball-mill (mixing mill MM 400 from Retsch). There were various vials used as containers; HPLC-vials were used for the small scale screening of stabilizers and injection vials for preparation of larger quantities of the nanosuspensions. Particle size distribution was analyzed by laser diffraction measurement (Mastersizer 2000). Lyophilization was used for processing of the suspensions (Christ freeze dryer). Stable nanosuspensions (d90 remained <1μm up to 7days) were prepared with several FDA-accepted stabilizers. Freeze drying was evaluated for one formulation containing 2% of the API, 0.5% of arginine and 4% of mannitol. The particle size distribution before freeze drying and after re-dispersion was comparable. After milling for 2h, no living microorganisms were detected in the nanosuspension. Various FDA accepted excipients were identified which resulted in stable nanosuspensions of BI XX. The most stable formulation was successfully freeze dried. It was proven that milling in the ball-mill decreases the presence of living microorganisms. Copyright © 2016. Published by Elsevier B.V.

Quantification of oxysterols in Dutch foods: egg products and mixed diets.

PubMed

van de Bovenkamp, P; Kosmeijer-Schuil, T G; Katan, M B

1988-11-01

A sensitive and specific method is described for quantifying various cholesterol oxidation products in foodstuffs, including 7 beta-hydroxycholesterol, cholesterol-alpha-epoxide, cholestane-triol, 7-ketocholesterol and 25-hydroxycholesterol. A chloroform-methanol extract of the food was fractionated over two successive silica columns. Two fractions containing different classes of oxysterols were then analyzed as trimethylsilyl derivatives by capillary gas liquid chromatography, using on-column injection and a temperature gradient from 70 to 200 degrees C. The detection limit was about 0.5 microgram/g dry weight for egg yolk powder. Fresh egg yolk contained only 1.2 micrograms/g of total oxides per g dry weight, showing that artifactual oxidation during the procedure was minimal. Recovery of 5 pure oxysterols added to egg yolk at levels of 6.5 and 10 micrograms/g was between 93 and 102%. In commercial egg yolk and whole egg powder stored for one year, total amounts of oxysterols ranging from 21 to 137 micrograms/g dry weight were found. In duplicates of mixed Dutch diets, total amounts ranged from 3.6 to 6.2 micrograms/g dry weight. Duplicates containing mostly fried and baked foods did not have higher levels than duplicates in which foods had been prepared by boiling or left raw. We conclude that a normal mixed diet provides only minor amounts of cholesterol oxidation products.

A Simple Carbon Dioxide Injection System for Photosynthetic Studies 12

PubMed Central

Oliver, David J.; Cameron, Stewart I.; Schaedle, Michail

1974-01-01

A simple carbon dioxide injection system has been developed for the maintenance of CO2 concentrations in semiclosed cuvette systems suitable for photosynthesis and gaseous pollutant studies. The device injects small volumes of pure carbon dioxide into the cuvette in response to a signal from an infrared gas analyzer. PMID:16658944

Design and development of a direct injection system for cryogenic engines

NASA Astrophysics Data System (ADS)

Mutumba, Angela; Cheeseman, Kevin; Clarke, Henry; Wen, Dongsheng

2018-04-01

The cryogenic engine has received increasing attention due to its promising potential as a zero-emission engine. In this study, a new robust liquid nitrogen injection system was commissioned and set up to perform high-pressure injections into an open vessel. The system is used for quasi-steady flow tests used for the characterisation of the direct injection process for cryogenic engines. An electro-hydraulic valve actuator provides intricate control of the valve lift, with a minimum cycle time of 3 ms and a frequency of up to 20 Hz. With additional sub-cooling, liquid phase injections from 14 to 94 bar were achieved. Results showed an increase in the injected mass with the increase in pressure, and decrease in temperature. The injected mass was also observed to increases linearly with the valve lift. Better control of the injection process, minimises the number of variables, providing more comparable and repeatable sets of data. Implications of the results on the engine performance were also discussed.

29 CFR Appendix C to Subpart L of... - Fire Protection References For Further Information

Code of Federal Regulations, 2010 CFR

2010-07-01

... National Standard for Occupational and Educational Eye and Face Protection, ANSI Z87.1; American National..., Batterymarch Park, Quincy, MA 02269 9. Standard for Dry Chemical Extinguishing Systems, ANSI/NFPA 17; National... systems—dry chemical: 1. Standard for Dry Chemical Extinguishing Systems, ANSI/NFPA 17; National Fire...

21 CFR 113.40 - Equipment and procedures.

Code of Federal Regulations, 2011 CFR

2011-04-01

... ensure a supply of clean, dry air. (3) Pressure gages. Each retort should be equipped with a pressure... should have adequate filter systems to ensure a supply of clean, dry air. (3) Pressure gages. (i) Each... controllers should have adequate filter systems to ensure a supply of clean, dry air. (3) Pressure gages. Each...

21 CFR 113.40 - Equipment and procedures.

Code of Federal Regulations, 2014 CFR

2014-04-01

... have adequate filter systems to ensure a supply of clean, dry air. A steam controller activated by the... ensure a supply of clean, dry air. (5) Steam introduction. Steam shall be distributed in the bottom of... temperature controllers should have adequate filter systems to ensure a supply of clean, dry air. (5) Bleeders...

21 CFR 113.40 - Equipment and procedures.

Code of Federal Regulations, 2013 CFR

2013-04-01

... have adequate filter systems to ensure a supply of clean, dry air. A steam controller activated by the... ensure a supply of clean, dry air. (5) Steam introduction. Steam shall be distributed in the bottom of... temperature controllers should have adequate filter systems to ensure a supply of clean, dry air. (5) Bleeders...

21 CFR 113.40 - Equipment and procedures.

Code of Federal Regulations, 2012 CFR

2012-04-01

... have adequate filter systems to ensure a supply of clean, dry air. A steam controller activated by the... ensure a supply of clean, dry air. (5) Steam introduction. Steam shall be distributed in the bottom of... temperature controllers should have adequate filter systems to ensure a supply of clean, dry air. (5) Bleeders...

Probing the biology of dry biological systems to address the basis of seed longevity

USDA-ARS?s Scientific Manuscript database

Drying cells reduces molecular mobility and slows chemical and physical reactions. As a result, dry biological systems deteriorate slowly. The time course of deterioration in a population of living cells often follows a sigmoidal pattern in which aging is occurring but no changes to viability are ...

Recent advances in drying and dehydration of fruits and vegetables: a review.

PubMed

Sagar, V R; Suresh Kumar, P

2010-01-01

Fruits and vegetables are dried to enhance storage stability, minimize packaging requirement and reduce transport weight. Preservation of fruits and vegetables through drying based on sun and solar drying techniques which cause poor quality and product contamination. Energy consumption and quality of dried products are critical parameters in the selection of drying process. An optimum drying system for the preparation of quality dehydrated products is cost effective as it shortens the drying time and cause minimum damage to the product. To reduce the energy utilization and operational cost new dimensions came up in drying techniques. Among the technologies osmotic dehydration, vacuum drying, freeze drying, superheated steam drying, heat pump drying and spray drying have great scope for the production of quality dried products and powders.

Spray drying from organic solvents to prepare nanoporous/nanoparticulate microparticles of protein: excipient composites designed for oral inhalation.

PubMed

Ní Ógáin, Orla; Tajber, Lidia; Corrigan, Owen I; Healy, Anne Marie

2012-09-01

The aim of this study was to determine if spray-drying could successfully produce microparticles containing the model protein trypsin in a form suitable for inhalation. Trypsin was spray-dried with raffinose from a methanol : n-butyl acetate solvent system (MeOH : BA). The solvent system was then adjusted to include water, and trypsin was co-spray-dried with raffinose, trehalose or hydroxpropyl-β-cyclodextrin. The spray-dried products were characterised by SEM, XRD, DSC, TGA and FTIR. Protein biological activity and in-vitro deposition of trypsin : excipient nanoporous/nanoparticulate microparticles (NPMPs) was also assessed. The inclusion of water in a MeOH : BA solvent system allowed for the successful production of NPMPs of trypsin : excipient by spray-drying. Trypsin formulated as trypsin : excipient NPMPs retained biological activity on processing and showed no deterioration in activity or morphological characteristics when stored with desiccant at either 4 or 25°C. Hydroxpropyl-β-cyclodextrin showed advantages over the sugars in terms of producing powders with appropriate density and with greater physical stability under high-humidity conditions. Fine particle fractions of between 41 and 45% were determined for trypsin : excipient NPMPs. NPMPs of trypsin : excipient systems can be produced by spray-drying by adjustment of the solvent system to allow for adequate solubility of trypsin. © 2012 The Authors. JPP © 2012 Royal Pharmaceutical Society.

Tracer-Encapsulated Solid Pellet (TESPEL) Injection System for the TJ-II Stellarator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tamura, N.; McCarthy, K. J.; Hayashi, H.

2016-01-01

A tracer-encapsulated solid pellet (TESPEL) injection system for the TJ-II stellarator was recently developed. In order to reduce the time and cost for the development, we combined a TESPEL injector provided by National Institute for Fusion Science with an existing TJ-II cryogenic pellet injection system. Consequently, the TESPEL injection into the TJ-II plasma was successfully achieved, which was confirmed by several pellet diagnostics including a normal-incidence spectrometer for monitoring a tracer impurity behavior.

Endotoxin increases pulmonary vascular protein permeability in the dog

DOE Office of Scientific and Technical Information (OSTI.GOV)

Welsh, C.H.; Dauber, I.M.; Weil, J.V.

Endotoxin increases pulmonary vascular permeability consistently in some species but fails to reliably cause injury in the dog. We wondered whether this phenomenon depended on the method of injury assessment, as others have relied on edema measurement; we quantified injury by monitoring the rate of extravascular protein accumulation. /sup 113m/In-labeled protein and /sup 99m/Tc-labeled erythrocytes were injected into anesthetized dogs and monitored by an externally placed lung probe. A protein leak index, the rate of extravascular protein accumulation, was derived from the rate of increase in lung protein counts corrected for changes in intravascular protein activity. After administration of Salmonellamore » enteriditis endotoxin (4 micrograms/kg), the protein leak index was elevated 2.5-fold (41.1 +/- 4.6 X 10(-4) min-1) compared with control (16.0 +/- 2.8 X 10(-4) min-1). In contrast, wet-to-dry weight ratios failed to increase after endotoxin (4.6 +/- 0.8 vs. control values of 4.2 +/- 0.5 g/g dry bloodless lung). However, we observed that endotoxin increased lung dry weight (per unit body weight), which may have attenuated the change in wet-to-dry weight ratios. To determine whether low microvascular pressures following endotoxin attenuated edema formation, we increased pulmonary arterial wedge pressures in five dogs by saline infusion, which caused an increase in wet-to-dry weight ratios following endotoxin but no change in the five controls. We conclude that low dose endotoxin causes pulmonary vascular protein leak in the dog while edema formation is minimal or absent.« less

Pore-network model of evaporation-induced salt precipitation in porous media: The effect of correlations and heterogeneity

NASA Astrophysics Data System (ADS)

Dashtian, Hassan; Shokri, Nima; Sahimi, Muhammad

2018-02-01

Salt transport and precipitation in porous media constitute a set of complex and fascinating phenomena that are of considerable interest to several important problems, ranging from storage of CO2 in geological formations, to soil fertility, and protection of pavements and roads, as well as historical monuments. The phenomena occur at the pore scale and are greatly influenced by the heterogeneity of the pore space morphology. We present a pore-network (PN) model to study the phenomena. Vapor diffusion, capillary effect at the brine-vapor interface, flow of brine, and transport of salt and its precipitation in the pores that plug the pores partially or completely are all accounted for. The drying process is modeled by the invasion percolation, while transport of salt in brine is accounted for by the convective-diffusion equation. We demonstrate that the drying patterns, the clustering and connectivity of the pore throats in which salt precipitation occurs, the saturation distribution, and the drying rate are all strongly dependent upon the pore-size distribution, the correlations among the pore sizes, and the anisotropy of the pore space caused by stratification that most natural porous media contain. In particular, if the strata are more or less parallel to the direction of injection of the gas that dries out the pore space (air, for example) and/or causes salt precipitation (CO2, for example), the drying rate increases significantly. Moreover, salt tends to precipitate in clusters of neighboring pores that are parallel to the open surface of the porous medium.

Waterflood control system for maximizing total oil recovery

DOEpatents

Patzek, Tadeusz Wiktor; Silin, Dimitriy Borisovic; De, Asoke Kumar

2005-06-07

A control system and method for determining optimal fluid injection pressure is based upon a model of a growing hydrofracture due to waterflood injection pressure. This model is used to develop a control system optimizing the injection pressure by using a prescribed injection goal coupled with the historical times, pressures, and volume of injected fluid at a single well. In this control method, the historical data is used to derive two major flow components: the transitional component, where cumulative injection volume is scaled as the square root of time, and a steady-state breakthrough component, which scales linearly with respect to time. These components provide diagnostic information and allow for the prevention of rapid fracture growth and associated massive water break through that is an important part of a successful waterflood, thereby extending the life of both injection and associated production wells in waterflood secondary oil recovery operations.

Waterflood control system for maximizing total oil recovery

DOEpatents

Patzek, Tadeusz Wiktor [Oakland, CA; Silin, Dimitriy Borisovich [Pleasant Hill, CA; De, Asoke Kumar [San Jose, CA

2007-07-24

A control system and method for determining optimal fluid injection pressure is based upon a model of a growing hydrofracture due to waterflood injection pressure. This model is used to develop a control system optimizing the injection pressure by using a prescribed injection goal coupled with the historical times, pressures, and volume of injected fluid at a single well. In this control method, the historical data is used to derive two major flow components: the transitional component, where cumulative injection volume is scaled as the square root of time, and a steady-state breakthrough component, which scales linearly with respect to time. These components provide diagnostic information and allow for the prevention of rapid fracture growth and associated massive water break through that is an important part of a successful waterflood, thereby extending the life of both injection and associated production wells in waterflood secondary oil recovery operations.

Quantification of antimicrobial consumption in adult cattle on dairy herds in Flanders, Belgium, and associations with udder health, milk quality, and production performance.

PubMed

Stevens, M; Piepers, S; Supré, K; Dewulf, J; De Vliegher, S

2016-03-01

The main objective of this study was to quantify the (compound-specific) antimicrobial consumption (AMC) in adult cattle in a convenience sample of Flemish dairy herds. Antimicrobial consumption data were obtained between 2012 and 2013 by "garbage can audits" and expressed as antimicrobial treatment incidence (ATI), with the unit of the ATI being the number of defined daily doses animal (DDDA) used per 1,000 cow-days. Herds were stratified by DDDA into low-, medium-, and high-consuming herds to study the AMC per route of administration, and associations with parameters reflecting udder health, milk quality, and production performances were examined. The average ATI in adult dairy cattle for all compounds was 20.78 DDDA (per 1,000 cow-days). Large variation existed between herds (ranging from 8.68 to 41.62 DDDA). Fourth-generation cephalosporins were used most (4.99 DDDA), followed by penicillins (3.70 DDDA) and third-generation cephalosporins (2.95 DDDA). The average ATI of the critically important antimicrobials for human health (i.e., third- and fourth-generation cephalosporins and fluoroquinolones according to the World Organisation for Animal Health classification) was somewhat lower than the average ATI of the other antimicrobials (8.59 and 12.18 DDDA, respectively). The average ATI for intramammary treatment of (sub)clinical mastitis, for dry-cow therapy, and for systemically administered antimicrobials was 6.30, 6.89, and 7.44 DDDA, respectively. In low-consuming herds, most antimicrobials were being used for dry-cow therapy, whereas in high-consuming herds, most antimicrobials were being used as injectable or intramammary mastitis therapy. The incidence rate of treated mastitis was positively associated with ATI. Herds that applied blanket dry-cow therapy tended to have a higher ATI than herds in which cows were selectively dried off with long-acting antimicrobials. The ATI decreased with an increasing prevalence of primiparous cows. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Root form and canal morphology of maxillary first premolars of a Yemeni population.

PubMed

Senan, Elham M; Alhadainy, Hatem A; Genaid, Thuraia M; Madfa, Ahmed A

2018-05-31

The purpose of this study was to investigate variations in the root canal systems of permanent maxillary first premolars in a Yemeni population using a clearing technique. Two hundred fifty permanent maxillary first premolar teeth extracted from Yemeni individuals were collected. A small hole in the center of the occlusal surface of each tooth was prepared and pulp tissue was removed by immersion in 5.25% sodium hypochlorite. Teeth were stored in 5-10% nitric acid solution for 5-6 days. Next, teeth were rinsed, dried, and dehydrated using ascending concentrations of ethanol (70, 95, and 100%) successively for 12 h each. Waterproof black ink was injected into the dried dehydrated teeth. Stained teeth were then rendered clear by immersion in methyl salicylate solution (98%) until evaluation. Root canal morphology of each tooth was then examined. 54.8% of teeth were single-rooted, while 44.4% were double-rooted and only 0.8% had three separated roots. The most common canal system configuration was Vertucci type IV (55.6%). Eight specimens of the single-rooted premolars (3.2%) had new canal configurations that have not been recognized in previous published studies. Accessory canals and inter-canal communications were detected in a total of 52.8 and 34.4% of the specimens, respectively. The apical foramen was located centrally to the apex in 84.9% and apical deltas were found in 13.2% of the studied sample. Yemeni permanent maxillary first premolars are mainly single-rooted and predominantly present Vertucci type IV canal morphology. The finding of additional canal configurations in this study is low but should be kept in mind when performing endodontic therapy for these teeth.

Engine with hydraulic fuel injection and ABS circuit using a single high pressure pump

DOEpatents

Bartley, Bradley E.; Blass, James R.; Gibson, Dennis H.

2001-01-01

An engine system comprises a hydraulically actuated fuel injection system and an ABS circuit connected via a fluid flow passage that provides hydraulic fluid to both the fuel injection system and to the ABS circuit. The hydraulically actuated system includes a high pressure pump. The fluid control passage is in fluid communication with an outlet from the high pressure pump.

Acute airway effects of airborne formaldehyde in sensitized and non-sensitized mice housed in a dry or humid environment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Larsen, Søren Thor, E-mail: stl@nrcwe.dk; Wolkoff, Peder, E-mail: pwo@nrcwe.dk; Hammer, Maria, E-mail: mha@nrcwe.dk

We investigated the role of air humidity and allergic sensitization on the acute airway response to inhaled formaldehyde (FA) vapor. Mice were sensitized to the immunogen ovalbumin (OVA) by three intraperitoneal injections followed by two aerosol challenges, giving rise to allergic airway inflammation. Control mice were sham sensitized by saline injections and challenged by saline aerosols. Once sensitized, the mice were housed at high (85–89%) or low (< 10%) relative humidity, respectively for 48 h prior to a 60-min exposure to either 0.4, 1.8 or about 5 ppm FA. Before, during and after exposure, breathing parameters were monitored. These includedmore » the specific markers of nose and lung irritations as well as the expiratory flow rate, the latter being a marker of airflow limitation. The sensory irritation response in the upper airways was not affected by allergic inflammation or changes in humidity. At high relative humidity, the OVA-sensitized mice had a decreased expiratory airflow rate compared to the saline control mice after exposure to approximately 5 ppm FA. This is in accordance with the observations that asthmatics are more sensitive than non-asthmatics to higher concentrations of airway irritants including FA. In the dry environment, the opposite trend was seen; here, the saline control mice had a significantly decreased expiratory airflow rate compared to OVA-sensitized mice when exposed to 1.8 and 4 ppm FA. We speculate that increased mucus production in the OVA-sensitized mice has increased the “scrubber effect” in the nose, consequently protecting the conducting and lower airways. - Highlights: ► Role of air humidity and allergy on sensitivity to an airway irritant was studied. ► In the humid environment, allergy amplified the effects of formaldehyde. ► In the dry environment, allergy reduced the effect of formaldehyde. ► Neither allergy nor humidity changed the formaldehyde-induced nasal irritation.« less

Hydrologic monitoring of a waste-injection well near Milton, Florida, June 1975 - December 1976

USGS Publications Warehouse

Pascale, Charles A.; Martin, J.B.

1977-01-01

Hydraulic and chemical data were collected through a monitoring program conducted by the U.S. Geological Survey at an industrial liquid-waste injection site 6 mi southwest of Milton, Fla., in Santa Rosa County. The injection system is described. Data include injection rates, volumes, and pressures; water-level data at three monitor wells and a standby injection well, and field and laboratory analyses of water samples from four wells. Hydraulic and geochemical effects of the waste-injection system at the plant as of December 31, 1976, have been detected only in the injection zone, the lower limestone of the Floridan aquifer. Increased pressures are evident at the three wells used to monitor the injection zone. Geochemical changes have been noted only at the deep-test monitor well closest to the injection well. (Woodard-USGS)