Dye lasing arrangement including an optical assembly for altering the cross-section of its pumping beam and method

DOEpatents

O'Neil, Richard W.; Sweatt, William C.

1992-01-01

An optical assembly is disclosed herein along with a method of operation for use in a dye lasing arrangement, for example a dye laser oscillator or a dye amplifier, in which a continuous stream of dye is caused to flow through a given zone in a cooperating dye chamber while the zone is being illuminated by light from a pumping beam which is directed into the given zone. This in turn causes the dye therein to lase and thereby produce a new dye beam in the case of a dye laser oscillator or amplify a dye beam in the case of a dye amplifier. The optical assembly so disclosed is designed to alter the pump beam such that the beam enters the dye chamber with a different cross-sectional configuration, preferably one having a more uniform intensity profile, than its initially produced cross-sectional configuration. To this end, the assembly includes a network of optical components which first act on the beam while the latter retains its initially produced cross-sectional configuration for separating it into a plurality of predetermined segments and then recombines the separated components in a predetermined way which causes the recombined beam to have the different cross-sectional configuration.

Composition and method of preparation of solid state dye laser rods

DOEpatents

Hermes, Robert E.

1992-01-01

The present invention includes solid polymeric-host laser rods prepared using bulk polymerization of acrylic acid ester comonomers which, when admixed with dye(s) capable of supporting laser oscillation and polymerized with a free radical initiator under mild thermal conditions, produce a solid product having the preferred properties for efficient lasing. Unsaturated polymerizable laser dyes can also be employed as one of the comonomers. Additionally, a method is disclosed which alleviates induced optical stress without having to anneal the polymers at elevated temperatures (>85.degree. C.).

Lasers '92; Proceedings of the International Conference on Lasers and Applications, 15th, Houston, TX, Dec. 7-10, 1992

NASA Technical Reports Server (NTRS)

Wang, Charles P. (Editor)

1993-01-01

Papers from the conference are presented, and the topics covered include the following: x-ray lasers, excimer lasers, chemical lasers, high power lasers, blue-green lasers, dye lasers, solid state lasers, semiconductor lasers, gas and discharge lasers, carbon dioxide lasers, ultrafast phenomena, nonlinear optics, quantum optics, dynamic gratings and wave mixing, laser radar, lasers in medicine, optical filters and laser communication, optical techniques and instruments, laser material interaction, and industrial and manufacturing applications.

Optical bending sensor using distributed feedback solid state dye lasers on optical fiber.

PubMed

Kubota, Hiroyuki; Oomi, Soichiro; Yoshioka, Hiroaki; Watanabe, Hirofumi; Oki, Yuji

2012-07-02

Novel type of optical fiber sensor was proposed and demonstrated. The print-like fabrication technique fabricates multiple distributed feedback solid state dye lasers on a polymeric optical fiber (POF) with tapered coupling. This multi-active-sidecore structure was easily fabricated and provides multiple functions. Mounting the lasers on the same point of a multimode POF demonstrated a bending radius sensitivity of 20 m without any supports. Two axis directional sensing without cross talk was also confirmed. A more complicated mounting formation can demonstrate a twisted POF. The temperature property of the sensor was also studied, and elimination of the temperature influence was experimentally attained.

Thin film DNA-complex-based dye lasers fabricated by immersion and conventional processes

NASA Astrophysics Data System (ADS)

Kawabe, Yutaka; Suzuki, Yuki

2017-08-01

DNA based thin film dye laser is one of promising optical devices for future technology. Laser oscillation and amplified spontaneous emission (ASE) were demonstrated by hemicyanine-doped DNA complex films prepared with `immersion method' as well as those made by a conventional way. In the immersion process, DNA-surfactant complex films were stained by immersion into an acetone solution including the dyes. In this study, three types of hemicyanines were incorporated with both methods, and laser oscillation was achieved with optically induced population grating formed in all of the complex films. The laser threshold values for six cases ranged in 0.07 - 0.18 mJ/cm2 , which was close to the best values made in DNA complex matrices. Continual pumping showed that laser oscillation persisted for 4 - 10 minutes. Immersion process gave superior laser capability especially for output efficiency over the conventional counterparts.

New ultra-high resolution dye laser spectrometer utilizing a non-tunable reference resonator

NASA Astrophysics Data System (ADS)

Helmcke, J.; Snyder, J. J.; Morinaga, A.; Mensing, F.; Gläser, M.

1987-06-01

A new dye laser spectrometer utilizing a non-tunable reference resonator is described. The resonator consists of two Zerodur mirrors optically contacted to a Zerodur spacer. Frequency scanning of the laser is provided by acoustooptic modulation. Residual drifts of the resonator frequency — measured on line — are compensated automatically by corresponding corrections of the modulation frequency. The stability during several hours and the resettability of the dye laser frequency are±2.5 kHz and±10 kHz, respectively.

Study of nonlinear refraction of organic dye by Z-scan technique using He-Ne laser

DOE Office of Scientific and Technical Information (OSTI.GOV)

Medhekar, S.; Kumar, R.; Mukherjee, S.

2013-02-05

Laser induced third-order nonlinear optical responses of Brilliant Green solution has been investigated by utilizing single beam Z-scan technique with a continuous-wave He-Ne laser radiation at 632.8 nm. It was observed that the material exhibits self-defocusing type optical nonlinearity. The measurements of nonlinear refraction were carried out at different dye concentrations and found that the increase in solution concentration leads to the linear increase of the nonlinear refractive index. The experimental results confirm great potential of the Brilliant Green for the application in nonlinear optical devices.

Laser inhibited diffusion in rhodamine-ethanol solutions

NASA Technical Reports Server (NTRS)

Lawandy, N. M.; Fuhr, P. L.; Robinson, D. W.

1981-01-01

The diffusion of rhodamine-6G dye in ethanol is observed to be inhibited by optical pumping by a cadmium laser. The diffusion process is observed as a function of the solution temperature. The relative difference in diffusion coefficients with and without optical pumping is calculated. The effect is interpreted as being due to a stronger solvent-dye interaction in the first excited singlet state of rhodamine-6G.

Photoactive dye-enhanced tissue ablation for endoscopic laser prostatectomy.

PubMed

Ahn, Minwoo; Hau, Nguyen Trung; Van Phuc, Nguyen; Oh, Junghwan; Kang, Hyun Wook

2014-11-01

Laser light has been widely used as a surgical tool to treat benign prostate hyperplasia (BPH) over 20 years. Recently, application of high laser power up to 200 W was often reported to swiftly remove a large amount of prostatic tissue. The purpose of this study was to validate the feasibility of photoactive dye injection to enhance light absorption and eventually to facilitate tissue vaporization with low laser power. Chicken breast tissue was selected as a target tissue due to minimal optical absorption at the visible wavelength. Four biocompatible photoactive dyes, including amaranth (AR), black dye (BD), hemoglobin powder (HP), and endoscopic marker (EM), were selected and tested in vitro with a customized 532 nm laser system with radiant exposure ranging from 0.9 to 3.9 J/cm(2) . Light absorbance and ablation threshold were measured with UV-Vis spectrometer and Probit analysis, respectively, and compared to feature the function of the injected dyes. Ablation performance with dye-injection was evaluated in light of radiant exposure, dye concentration, and number of injection. Higher light absorption by injected dyes led to lower ablation threshold as well as more efficient tissue removal in the order of AR, BD, HP, and EM. Regardless of the injected dyes, ablation efficiency principally increased with radiant exposure, dye concentration, and number of injection. Among the dyes, AR created the highest ablation rate of 44.2 ± 0.2 µm/pulse due to higher absorbance and lower ablation threshold. High aspect ratios up to 7.1 ± 0.4 entailed saturation behavior in the tissue ablation injected with AR and BD, possibly resulting from plume shielding and increased scattering due to coagulation. Preliminary tests on canine prostate with a hydraulic injection system demonstrated that 80 W with dye injection yielded comparable ablation efficiency to 120 W with no injection, indicating 33% reduced laser power with almost equivalent performance. Due to efficient coupling of optical energy, pre-injection of photoactive dyes promoted the degree of tissue removal during laser irradiation. Further studies will investigate spatial distribution of dyes and optimal injecting pressure to govern the extent of dye-assisted ablation in a predictable manner. In-depth comprehension on photoactive dye-enhanced tissue ablation can help accomplish efficient and safe laser vaporization for BPH with low power application. © 2014 Wiley Periodicals, Inc.

Third-order nonlinear optical properties of organic azo dyes by using strength of nonlinearity parameter and Z-scan technique

NASA Astrophysics Data System (ADS)

Motiei, H.; Jafari, A.; Naderali, R.

2017-02-01

In this paper, two chemically synthesized organic azo dyes, 2-(2,5-Dichloro-phenyazo)-5,5-dimethyl-cyclohexane-1,3-dione (azo dye (i)) and 5,5-Dimethyl-2-tolylazo-cyclohexane-1,3-dione (azo dye (ii)), have been studied from optical Kerr nonlinearity point of view. These materials were characterized by Ultraviolet-visible spectroscopy. Experiments were performed using a continous wave diode-pumped laser at 532 nm wavelength in three intensities of the laser beam. Nonlinear absorption (β), refractive index (n2) and third-order susceptibility (χ (3)) of dyes, were calculated. Nonlinear absorption coefficient of dyes have been calculated from two methods; 1) using theoretical fits and experimental data in the Z-scan technique, 2) using the strength of nonlinearity curves. The values of β obtained from both of the methods were approximately the same. The results demonstrated that azo dye (ii) displays better nonlinearity and has a lower two-photon absorption threshold than azo dye (i). Calculated parameter related to strength of nonlinearity for azo dye (ii) was higher than azo dye (i), It may be due to presence of methyl in azo dye (ii) instead of chlorine in azo dye (i). Furthermore, The measured values of third order susceptibility of azo dyes were from the order of 10-9 esu . These azo dyes can be suitable candidate for optical switching devices.

Random lasing from dye-doped negative liquid crystals using ZnO nanoparticles as tunable scatters

NASA Astrophysics Data System (ADS)

Li, Long-Wu; Shang, Zhen-Zhen; Deng, Luogen

2016-09-01

This work demonstrates the realization of a lasing in scattering media, which contains dispersive solution of ZnO nanoparticles (NPs) and laser dye 4-dicyanomethylene-2-methyle-6-(p-dimethylaminostyryl)-4H-pyran(DCM) in negative liquid crystals (LCs) that was injected into a cell. The lasing intensity of the dye-doped negative LC laser can be tuned from low to high if the NPs concentration is increased. The tunability of the laser is attributable to the clusters-sensitive feature in effective refractive index of the negative LCs. Such a tunable negative liquid crystal laser can be used in the fabrication of new optical sources, optical communication, and liquid crystal laser displays. Project supported by the Doctoral Science Research Start-up Funding of Guizhou Normal University, China (Grant No. 11904-0514162) and the National Natural Science Foundation of China (Grant No. 11474021).

Infrared Dyes For Optical Storage

NASA Astrophysics Data System (ADS)

Jipson, V. B.; Jones, C. R.

1981-06-01

There is current interest in developing optical storage materials that can be written with GaAlAs lasers. Dyes which absorb strongly at those wavelengths are potential candidates for this application due to their attractive thermal properties. Through optical and thermal modelling, the properties that are necessary if they are to be writeable at energies of <=1 nJ are examined. A specific class of infrared absorbing dyes, squarylium, is discussed and preliminary data on optical characteristics, writing energy, and stability are presented.

Z-scan and optical limiting properties of Hibiscus Sabdariffa dye

NASA Astrophysics Data System (ADS)

Diallo, A.; Zongo, S.; Mthunzi, P.; Rehman, S.; Alqaradawi, S. Y.; Soboyejo, W.; Maaza, M.

2014-12-01

The intensity-dependent refractive index n 2 and the nonlinear susceptibility χ (3) of Hibiscus Sabdariffa dye solutions in the nanosecond regime at 532 nm are reported. More presicely, the variation of n 2, β, and real and imaginary parts of χ (3) versus the natural dye extract concentration has been carried out by z-scan and optical limiting techniques. The third-order nonlinearity of the Hibiscus Sabdariffa dye solutions was found to be dominated by nonlinear refraction, which leads to strong optical limiting of laser.

Optical Properties of Nano-Spherical Gold Doped Dye Solution Hybrid

NASA Astrophysics Data System (ADS)

Hoa, D. Q.; Lien, N. T. H.; Ha, C. V.; Nhung, T. H.; Long, P.

2011-03-01

Gold nanoparticles with average diameter of 16 nm which are coated with Cetrimonium Bromide (CTAB) by chemical method are dissolved in dye solution at different concentrations. The absorption spectra of the dye mixture appeared almost unchanged at low concentrations of gold nanoparticles (around 1×1014 cm-3) despite its fluorescence intensity increased many-fold. Energy transfer from gold nanoparticles to dye molecules occurs through surface plasmon resonance(SPR). The fluorescence of rhodamine 610 (Rh610) dye molecules co-adsorbed within 16 nm gold nanoparticles assemblies can be useful for enhancing gain in lasing emission. An increase in laser efficiency by a factor of one and half times stronger compared to the single Rh610 dye suggest the potential of using the mixture of rhodamine dye with gold nanoparticles as laser medium in the configuration of quenching distributed feedback dye laser.

Development of high-power dye laser chain

NASA Astrophysics Data System (ADS)

Konagai, Chikara; Kimura, Hironobu; Fukasawa, Teruichiro; Seki, Eiji; Abe, Motohisa; Mori, Hideo

2000-01-01

Copper vapor laser (CVL) pumped dye laser (DL) system, both in a master oscillator power amplifier (MOPA) configuration, has been developed for Atomic Vapor Isotope Separation program in Japan. Dye laser output power of about 500 W has been proved in long-term operations over 200 hours. High power fiber optic delivery system is utilized in order to efficiently transport kilowatt level CVL beams to the DL MOPA. Single model CVL pumped DL oscillator has been developed and worked for 200 hours within +/- 0.1 pm wavelength stability. Phase modulator for spreading spectrum to the linewidth of hyperfine structure has been developed and demonstrated.

Luminescent characteristics study of Mather-type dense plasma focus and applications to short-wavelength optical pumping. Final technical report, 1 May 1984-30 September 1985

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, K.K.

A Mather-type dense plasma focus (MDPF) system was designed, built, and tested specifically to study its luminescent characteristics and to assess its potential as a new light source of high-energy, short-wavelength lasers. The luminescence study of MDPF showed that the conversion efficiency from the electrical input to the optical output energies is at least 50%, up to the time the plasma compression is complete. Using the system, for the first time as an optical pump, laser activities were successfully obtained from a variety of liquid organic dyes. Diagnostic capabilities included an optical multichannel analyzer system complete with a computer control,more » a nitrogen-pumped tunable dye-laser system, a high-speed streak/framing camera, a digital laser energy meter, voltage and current probes, and a computer-based data-acquisition system.« less

Cellular dye lasers: lasing thresholds and sensing in a planar resonator

PubMed Central

Humar, Matjaž; Gather, Malte C.; Yun, Seok-Hyun

2015-01-01

Biological cell lasers are promising novel building blocks of future biocompatible optical systems and offer new approaches to cellular sensing and cytometry in a microfluidic setting. Here, we demonstrate a simple method for providing optical gain by using a variety of standard fluorescent dyes. The dye gain medium can be located inside or outside a cell, or in both, which gives flexibility in experimental design and makes the method applicable to all cell types. Due to the higher refractive index of the cytoplasm compared to the surrounding medium, a cell acts as a convex lens in a planar Fabry-Perot cavity. Its effect on the stability of the laser cavity is analyzed and utilized to suppress lasing outside cells. The resonance modes depend on the shape and internal structure of the cell. As proof of concept, we show how the laser output modes are affected by the osmotic pressure. PMID:26480446

Effects of vacuum exposure on stress and spectral shift of high reflective coatings

NASA Astrophysics Data System (ADS)

Stolz, C. J.; Taylor, J. R.; Eickelberg, W. K.; Lindh, J. D.

1992-06-01

The Atomic Vapor Laser Isotope Laser Separation (AVLIS) program operates the world's largest average power dye laser; the dye laser beams are combined, formatted, and transported in vacuum. The optical system is aligned at atmosphere, while the system must meet requirements in vacuum. Therefore, coating performance must be characterized in both atmosphere and vacuum. Changes in stress and spectral shift in ambient and vacuum environments are reported for conventional and dense multilayer dielectric coatings.

Enhanced optical confinement of dye-doped dielectric nanoparticles using a picosecond-pulsed near-infrared laser

NASA Astrophysics Data System (ADS)

Kittiravechote, A.; Chiang, W.-Y.; Usman, A.; Liau, I.; Masuhara, H.

2014-07-01

We demonstrate a novel strategy to increase the capability of confining numerous dye-doped polymeric nanobeads (diameter 100 nm) with laser trapping. Unlike most classical works of optical trapping that address mainly the stiffness of the optical trap, our work concerns an increase in the number of particles confined near the laser focus. We developed an imaging system of light scattering in which a condenser lamp was employed to illuminate the focal plane of the objective lens, and the scattering of the incoherent light was specifically measured to determine the number of confined nanobeads. In contrast to preceding work that used mainly continuous-wave or femtosecond-pulsed lasers, we employed a picosecond-pulsed laser with the half-wavelength of the laser particularly falling within the absorption band of the dopant. Our results show that the number of doped nanobeads held by the laser is significantly greater than that of the bare nanobeads of the same dimension. In striking contrast, the confinement of the nanobeads of the two types was comparable when a continuous-wave laser of the same wavelength and power was employed. The number of confined dye-doped nanobeads increased nonlinearly with the power of the pulsed laser; this dependence was fitted satisfactorily with a second-order polynomial. Supported by theoretical analysis, we attribute the enhanced confinement of doped nanobeads in part to an increased effective refractive index resulting from two-photon resonance between the optical field of the laser and the dopant of the nanobead. We envisage that our findings would evoke applications that benefit from controlled confinement or aggregation of nanomaterials with the employment of near-infrared pulsed lasers.

All-Union Conference on Laser Optics, 4th, Leningrad, USSR, January 13-18, 1984, Proceedings

NASA Astrophysics Data System (ADS)

Bukhenskii, M. F.

1984-08-01

The papers presented in this volume provide an overview of current theoretical and experimental research in laser optics. Topics discussed include electronically controlled tunable lasers, nonlinear phenomena in fiber-optic waveguides, holographic distributed-feedback dye lasers, and new developments in solid-state lasers. Papers are also presented on the generation of picosecond pulses through self-Q-switching in a distributed-feedback laser, temporal compression of light pulses during stimulated backscattering, and optimization of second harmonic generation in a multimode Nd:glass laser.

Turnable Blue-Green LIDAR Transmitter Demonstration: Injection Laser Technology

DTIC Science & Technology

1990-08-30

5-1 5.2 Baseline Requirements ............................................. 5-1 5.3 Optical Parametric Oscillator Using Beta Barium Borate... optical parametric oscillators , and organic dye lasers. Tunable solid state lasers such as Ti: sapphire operate in the infrared and would have to be...The same is true of I frequency mixing schemes. Optical parametric oscillators (OPOs) are attractive because of their extremely wide potential tuning

Method for changing the cross section of a laser beam

DOEpatents

Sweatt, W.C.; Seppala, L.

1995-12-05

A technique is disclosed herein in which a circular optical beam, for example a copper vapor laser (CVL) beam, is converted to a beam having a profile other than circular, e.g. square or triangular. This is accomplished by utilizing a single optical mirror having a reflecting surface designed in accordance with a specifically derived formula in order to make the necessary transformation, without any substantial light loss and without changing substantially the intensity profile of the circular beam which has a substantially uniform intensity profile. In this way, the output beam can be readily directed into the dye cell of a dye laser. 4 figs.

Method for changing the cross section of a laser beam

DOEpatents

Sweatt, William C.; Seppala, Lynn

1995-01-01

A technique is disclosed herein in which a circular optical beam, for example a copper vapor laser (CVL) beam, is converted to a beam having a profile other than circular, e.g. square or triangular. This is accomplished by utilizing a single optical mirror having a reflecting surface designed in accordance with a specifically derived formula in order to make the necessary transformation, without any substantial light loss and without changing substantially the intensity profile of the circular beam which has a substantially uniform intensity profile. In this way, the output beam can be readily directed into the dye cell of a dye laser.

Photoactive dye enhanced tissue ablation for endoscopic laser prostatectomy

NASA Astrophysics Data System (ADS)

Ahn, Minwoo; Nguyen, Trung Hau; Nguyen, Van Phuc; Oh, Junghwan; Kang, Hyun Wook

2015-02-01

Laser light has been widely used as a surgical tool to treat benign prostate hyperplasia with high laser power. The purpose of this study was to validate the feasibility of photoactive dye injection to enhance light absorption and eventually to facilitate tissue ablation with low laser power. The experiment was implemented on chicken breast due to minimal optical absorption Amaranth (AR), black dye (BD), hemoglobin powder (HP), and endoscopic marker (EM), were selected and tested in vitro with a customized 532-nm laser system with radiant exposure ranging from 0.9 to 3.9 J/cm2. Light absorbance and ablation threshold were measured with UV-VIS spectrometer and Probit analysis, respectively, and compared to feature the function of the injected dyes. Ablation performance with dye-injection was evaluated in light of radiant exposure, dye concentration, and number of injection. Higher light absorption by injected dyes led to lower ablation threshold as well as more efficient tissue removal in the order of AR, BD, HP, and EM. Regardless of the injected dyes, ablation efficiency principally increased with input parameter. Among the dyes, AR created the highest ablation rate of 44.2+/-0.2 μm/pulse due to higher absorbance and lower ablation threshold. Preliminary tests on canine prostate with a hydraulic injection system demonstrated that 80 W with dye injection yielded comparable ablation efficiency to 120 W with no injection, indicating 33 % reduced laser power with almost equivalent performance. In-depth comprehension on photoactive dye-enhanced tissue ablation can help accomplish efficient and safe laser treatment for BPH with low power application.

Transforming Benzophenoxazine Laser Dyes into Chromophores for Dye-Sensitized Solar Cells: A Molecular Engineering Approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schröder, Florian A. Y. N.; Cole, Jacqueline M.; Waddell, Paul G.

2015-02-03

The re-functionalization of a series of four well-known industrial laser dyes, based on benzophenoxazine, is explored with the prospect of molecularly engineering new chromophores for dye-sensitized solar cell (DSC) applications. Such engineering is important since a lack of suitable dyes is stifling the progress of DSC technology. The conceptual idea involves making laser dyes DSC-active by chemical modification, while maintaining their key property attributes that are attractive to DSC applications. This molecular engineering follows a step-wise approach. Firstly, molecular structures and optical absorption properties are determined for the parent laser dyes: Cresyl Violet (1); Oxazine 170 (2); Nile Blue Amore » (3), Oxazine 750 (4). These reveal structure-property relationships which define the prerequisites for computational molecular design of DSC dyes; the nature of their molecular architecture (D-π-A) and intramolecular charge transfer. Secondly, new DSC dyes are computationally designed by the in silico addition of a carboxylic acid anchor at various chemical substitution points in the parent laser dyes. A comparison of the resulting frontier molecular orbital energy levels with the conduction band edge of a TiO2 DSC photoanode and the redox potential of two electrolyte options I-/I3- and Co(II/III)tris(bipyridyl) suggests promise for these computationally designed dyes as co-sensitizers for DSC applications.« less

Rationalizing the photophysical properties of BODIPY laser dyes via aromaticity and electron-donor-based structural perturbations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Waddell, Paul G.; Liu, Xiaogang; Zhao, Teng

2015-05-01

The absorption and fluorescence properties of six boron dipyrromethene (BODIPY) laser dyes with simple non-aromatic substituents are rationalized by relating them to observable structural perturbations within the molecules of the dyes. An empirical relationship involving the structure and the optical properties is derived using a combination of single-crystal X-ray diffraction data, quantum chemical calculations and electronic constants: i.e. the tendency of the pyrrole bond lengths towards aromaticity and the UV-vis absorption and fluorescence wavelengths correlating with the electron-donor properties of the substituents. The effect of molecular conformation on the solid-state optical properties of the dyes is also discussed. The findingsmore » in this study also demonstrate the usefulness and limitations of using crystal structure data to develop structure-property relationships in this class of optical materials, contributing to the growing effort to design optoelectronic materials with tunable properties via molecular engineering.« less

Investigation of laser induced parametric interactions in optical waveguides and fibers

NASA Technical Reports Server (NTRS)

Yu, C.

1978-01-01

Experimental and theoretical aspects of the laser pump depletion characteristics in an optical fiber due to stimulated Raman scattering, and stimulated Brillouin scattering were studied. A review is presented of research in fiber transmission accompanied by stimulated scattering. Results of experimental work with tunable dye lasers and argon lasers are presented. The spectral profiles of the laser pump and its transmitted light through the fiber are given.

Random lasing action in a polydimethylsiloxane wrinkle induced disordered structure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shen, Zhenhua; Wu, Leilei; Zhu, Shu

This paper presents a chip-scale random lasing action utilizing polydimethylsiloxane (PDMS) wrinkles with random periods as disordered medium. Nanoscale wrinkles with long range disorder structures are formed on the oxidized surface of a PDMS slab and confirmed by atomic force microscopy. Light multiply scattered at each PDMS wrinkle-dye interfaces is optically amplified in the presence of pump gain. The shift of laser emission wavelength when pumping at different regions indicates the randomness of the winkle period. In addition, a relatively low threshold of about 27 μJ/mm{sup 2} is realized, which is comparable with traditional optofluidic dye laser. This is due tomore » the unique sinusoidal Bragg-grating-like random structure. Contrast to conventional microfluidic dye laser that inevitably requires the accurate design and implementation of microcavity to provide optical feedback, the convenience in both fabrication and operation makes PDMS wrinkle based random laser a promising underlying element in lab-on-a-chip systems and integrated microfluidic networks.« less

Optical study of xanthene-type dyes in nano-confined liquid

NASA Astrophysics Data System (ADS)

Mahdi Shavakandi, Seyyed; Alizadeh, Khalil; Sharifi, Soheil; Marti, Othmar; Amirkhani, Masoud

2017-04-01

The optical activity of dye molecules in different environments is of great interest for many applications such as laser system or biological imaging. We investigate the fluorescence and absorption spectrum of nano-confined xanthene dyes (RhB and fluorescein sodium salt) in a two-phase liquid. Each show very distinct optical behavior in the water phase of a reverse microemulsion. Their optical properties such as absorption and fluorescence for different concentrations of dye and nanodroplets are investigated. We show that for the same concentration of dye in the microemulsion the peak of fluorescence intensity is varied by altering the concentration of nanodroplets. However, the trend of the change is widely different depending on the hydrophobicity of dyes. Quantum-mechanical second order perturbation theory is used to calculate the ratio of dipole moments in the ground and excited states, which accounts for the Stokes shift in fluorescence peak. Photon correlation spectroscopy is employed to check the trace of the dye in the oil phase of the microemulsion.

Digital DNA detection based on a compact optofluidic laser with ultra-low sample consumption.

PubMed

Lee, Wonsuk; Chen, Qiushu; Fan, Xudong; Yoon, Dong Ki

2016-11-29

DNA lasers self-amplify optical signals from a DNA analyte as well as thermodynamic differences between sequences, allowing quasi-digital DNA detection. However, these systems have drawbacks, such as relatively large sample consumption and complicated dye labelling. Moreover, although the lasing signal can detect the target DNA, it is superimposed on an unintended fluorescence background, which persists for non-target DNA samples as well. From an optical point of view, it is thus not truly digital detection and requires spectral analysis to identify the target. In this work, we propose and demonstrate an optofluidic laser that has a single layer of DNA molecules as the gain material. A target DNA produces intensive laser emission comparable to existing DNA lasers, while any unnecessary fluorescence background is successfully suppressed. As a result, the target DNA can be detected with a single laser pulse, in a truly digital manner. Since the DNA molecules cover only a single layer on the surface of the laser microcavity, the DNA sample consumption is a few orders of magnitude lower than that of existing DNA lasers. Furthermore, the DNA molecules are stained by simply immersing the microcavity in the intercalating dye solution, and thus the proposed DNA laser is free of any complex dye-labelling process prior to analysis.

Unconventional High-Performance Laser Protection System Based on Dichroic Dye-Doped Cholesteric Liquid Crystals

NASA Astrophysics Data System (ADS)

Zhang, Wanshu; Zhang, Lanying; Liang, Xiao; Le Zhou; Xiao, Jiumei; Yu, Li; Li, Fasheng; Cao, Hui; Li, Kexuan; Yang, Zhou; Yang, Huai

2017-02-01

High-performance and cost-effective laser protection system is of crucial importance for the rapid advance of lasers in military and civilian fields leading to severe damages of human eyes and sensitive optical devices. However, it is crucially hindered by the angle-dependent protective effect and the complex preparation process. Here we demonstrate that angle-independence, good processibility, wavelength tunability, high optical density and good visibility can be effectuated simultaneously, by embedding dichroic anthraquinone dyes in a cholesteric liquid crystal matrix. More significantly, unconventional two-dimensional parabolic protection behavior is reported for the first time that in stark contrast to the existing protection systems, the overall parabolic protection behavior enables protective effect to increase with incident angles, hence providing omnibearing high-performance protection. The protective effect is controllable by dye concentration, LC cell thickness and CLC reflection efficiency, and the system can be made flexible enabling applications in flexible and even wearable protection devices. This research creates a promising avenue for the high-performance and cost-effective laser protection, and may foster the development of optical applications such as solar concentrators, car explosion-proof membrane, smart windows and polarizers.

Bibliography of Soviet Laser Developments, Number 28, March - April 1977.

DTIC Science & Technology

1978-02-24

Dyes " a. Rhodamine 25. Makogon, M.M. and V.B. Sukhanov (0). Dye laser with a partially se- lective resonator. ZhPS, v.26, no.4, 1977, 622-625. b...medium. KE, no.4, 1977, 751-757. 105. Bel’tyugov, V.N., I.I. Sukhanov , and Yu.V. Troitskiy (0). Thermal dis- tortions in intraresonator optical

Intense Excitation Source of Blue-Green Laser.

DTIC Science & Technology

1985-10-15

plasma focus (DPF) can produce intense uv photons (200-300nm) which match the absorption spectra of both near uv and blue green dye lasers (300-400nm...existing blue green dye laser. On the other hand the dense- plasma focus (DPF) with new optical coupling has been designed and constructed. For the...optimization of the DPF device as the uv pumping light source, the velocity of current sheath and the formation of plasma focus have been measured as

Performance of a nonlaser light source for photodynamic therapy

NASA Astrophysics Data System (ADS)

Whitehurst, Colin; Byrne, Karen T.; Morton, Colin; Moore, James V.

1995-03-01

Advances in short arc technology and optical filter coatings led to the design and construction of a table-top light source in 1989; the first viable and cost-effective alternative to a laser. The device can deliver over 3 W within a 30 nm band centered at any wavelength from 200 nm to 1200 nm at fluence rates of over 1 W cm-2. Its relative biological effectiveness (RBE) in vitro has been proven alongside an argon pumped dye laser and a copper vapor pumped dye laser. These in vitro tests showed an efficiency of hematoporphyrin derivative (HPD) induced cellular photoinactivation close to that of the argon/dye laser (RBE 100%), with a mean RBE for the lamp of 87 +/- 3% (p < 0.05). The lamp proved to be superior to that of the copper/dye laser system with an RBE of up to 150% at fluence rates above 50 mWcm-2. In vivo tests show that the extent and depth of tumor necrosis are comparable to that of an argon/dye laser. An in situ bioassay using tumor regrowth delay is currently underway. Early clinical trials show clearance of Bowen's disease and actinic keratosis using the same light fluences as costly PDT lasers.

Micromanipulation and physiological monitoring of cells using two-photon excited fluorescence in cw laser tweezers

NASA Astrophysics Data System (ADS)

Sonek, Gregory J.; Liu, Yagang; Berns, Michael W.; Tromberg, Bruce J.

1996-05-01

We report the observation of two-photon fluorescence excitation and cell confinement, simultaneously, in a continuous-wave (cw) single-beam gradient force optical trap, and demonstrate its use as an in-situ probe to study the physiological state of an optically confined cell sample. At the wavelength of 1064 nm, a single focused gaussian laser beam is used to simultaneously confine, and excite visible fluorescence from, a human sperm cell that has been tagged with propidium iodide, a exogenous fluorescent dye that functions as a viability assay of cellular physiological state. The intensity at the dye peak emission wavelength of 620 nm exhibits a near-square-law dependence on incident trapping beam photon laser power, a behavior consistent with a two-photon absorption process. In addition, for a sperm cell held stationary in the optical tweezers for a period of several minutes at a constant trapping power, red fluorescence emission was observed to increase the time, indicating that the cell has gradually transitioned between a live and dead state. Two-photon excited fluorescence was also observed in chinese hamster ovary cells that were confined by cw laser tweezers and stained with either propidium iodide or Snarf, a pH-sensitive dye probe. These results suggest that, for samples suitably tagged with fluorescent probes and vital stains, optical tweezers can be used to generate their own in-situ diagnostic optical probes of cellular viability or induced photodamage, via two-photon processes.

Enhanced optical, thermal and piezoelectric behavior in dye doped potassium acid phthalate (KAP) single crystal

NASA Astrophysics Data System (ADS)

Rao, G. Babu; Rajesh, P.; Ramasamy, P.

2017-06-01

Dye inclusion crystals have attracted researchers in the context of crystal growth for applications in solid state lasers. Pure and 0.1 mol% amaranth doped KAP single crystals, were grown from aqueous solutions by slow evaporation technique at room temperature. The grown crystals are up to the dimension of 12×10×3 mm3. Attempt is made to improve the growth rate, optical, piezoelectric and photoconductive properties of pure KAP single crystal with addition of amaranth dye as a dopant. Various characterization studies were made for both pure and dye doped KAP. Thermal stability of the crystals is tested from thermogravimetric and differential thermal analysis (TG/DTA). There is only one endothermic peak indicating decomposition point. Higher optical transparency for dye doped KAP crystal was identified from the UV-vis spectrum. Etching studies showed an improvement in the optical quality of the KAP crystal after doping with amaranth dye. The positive photoconductive nature is observed from both pure and amaranth doped KAP.

Applications of lasers and electro-optics

NASA Astrophysics Data System (ADS)

Tan, B. C.; Low, K. S.; Chen, Y. H.; Ahmad, Harith; Tou, T. Y.

Supported by the IRPA Programme on Laser Technology and Applications, many types of lasers have been designed, constructed and applied in various areas of science, medicine and industries. Amongst these lasers constructed were high power carbon dioxide lasers, rare gas halide excimer lasers, solid state Neodymium-YAG lasers, nitrogen lasers, flashlamp pumped dye lasers and nitrogen and excimer laser pumped dye lasers. These lasers and the associated electro-optics system, some with computer controlled, are designed and developed for the following areas of applications: (1) industrial applications of high power carbon dioxide lasers for making of i.c. components and other materials processing purposes -- prototype operational systems have been developed; (2) Medical applications of lasers for cancer treatment using the technique of photodynamic therapy -- a new and more effective treatment protocol has been proposed; (3) agricultural applications of lasers in palm oil and palm fruit-fluorescence diagnostic studies -- fruit ripeness signature has been developed and palm oil oxidation level were investigated; (4) development of atmospheric pollution monitoring systems using laser lidar techniques -- laboratory scale systems were developed; and (5) other applications of lasers including laser holographic and interferometric methods for the non destructive testing of materials.

Master dye laser oscillator including a specific grating assembly for use therein

DOEpatents

Davin, James M.

1992-01-01

A dye laser oscillator for producing a tuned dye beam is disclosed herein and includes, among other components, a beam output coupling assembly, a dye cell assembly, a beam expander assembly, an etalon assembly, and a grating assembly. Each of three assemblies is vertically supported from a horizontal base so as to be readily removable from the base without interference from or interfering with the other assemblies. The particular grating assembly disclosed is specifically designed for proper optical alignment with the intended path of the dye beam to be produced and for accurate pivotal movement relative to the beam path in order to function as a coarse tuning mechanism in the production of the ultimately tuned beam.

Master dye laser oscillator including a specific grating assembly for use therein

DOEpatents

Davin, J.M.

1992-09-01

A dye laser oscillator for producing a tuned dye beam is disclosed herein and includes, among other components, a beam output coupling assembly, a dye cell assembly, a beam expander assembly, an etalon assembly, and a grating assembly. Each of three assemblies is vertically supported from a horizontal base so as to be readily removable from the base without interference from or interfering with the other assemblies. The particular grating assembly disclosed is specifically designed for proper optical alignment with the intended path of the dye beam to be produced and for accurate pivotal movement relative to the beam path in order to function as a coarse tuning mechanism in the production of the ultimately tuned beam. 5 figs.

Mather-type dense plasma focus as a new optical pump for short-wavelength high-power lasers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fanning, J.J.; Kim, K.

For the first time, a Mather-type dense plasma focus (MDPF) is successfully operated as an optical pump for lasers. Rhodamine-6G dye is optically pumped using the MDPF fluorescence, producing a laser pulse 1 ..mu..s in duration and more than 50 kW in output power. No optimization is attempted either of the laser cavity or of the lasing medium concentration and volume. A brief description of the experimental setup is presented, along with a summary and discussion of the results. The advantages of the present optical pump source and, in particular, their implications for the pumping of short-wavelength lasers are discussed.

Nonlinearities of polymethine and squarylium molecules for optical limiting

NASA Astrophysics Data System (ADS)

Lim, Jin Hong

Optical limiting, a process that reduces transmittance at high laser input energies (irradiance, fluence), is of interest in applications where sensitive optical components, e.g. detectors, are vulnerable to damage by the laser beam. Polymethine and squarylium dyes show strong reverse saturable absorption (RSA) at 532 nm. RSA is a process by which weak linear absorption populates excited states which subsequently absorb strongly. Thus, low inputs are transmitted while high inputs are absorbed. This nonlinear absorption is determined by the ground and excited-state absorption cross sections as well as excited state lifetimes of the molecular system. We characterized a series of polymethine and squarine molecules in ethanol and polyurethane acrylate polymeric host (PUA) using Z-scan and pump-probe techniques at the second harmonic of the Nd:YAG laser system. A comparison of the properties in these two hosts is made. Some of these dyes show a large ratio of excited to ground state absorption cross section, ~200, which is larger than any previously reported values. In order to determine the wavelength dependence of the nonlinearities of these molecules, we also performed Z-scan and pump-probe experiments at wavelengths from 440 to 650 nm using a picosecond optical parametric oscillator (OPO) which is synchronously pumped by the third-harmonic of a modelocked train of Nd:YAG laser pulses. The OPO is continuously tunable from 400 to 700 nm using two critically phase-matched BBO crystals mounted for walkoff compensation. A polymethine dye in PUA (PD #3), which is one of the best polymethine dyes at 532 nm, shows strong RSA over a broad spectral range from 480 to 620 nm. while a squarylium dye shows RSA over a relatively narrow spectral range from 500 to 560 nm. However, the excited state lifetimes (~2.5 ns in PUA) are shorter than desirable for good nanosecond optical limiting (10 ns) and at high inputs (>=0.36 J/cm2) the limiting properties are reduced. Extensive measurements of these molecules along with computer modeling indicate that the reduced limiting at high inputs is due to molecular degradation induced after a trans-cis conformational change. Evidence for this and possible methods to eliminate this problem are presented.

Liquid crystal emulsion micro-droplet WGM resonators

NASA Astrophysics Data System (ADS)

Ježek, Jan; Pilát, Zdeněk.; Brzobohatý, Oto; Jonáš, Alexandr; Aas, Mehdi; Kiraz, Alper; Zemánek, Pavel

2014-12-01

We introduce tunable optofluidic microlasers based on optically stretched or thermally modified, dye-doped emulsion droplets of liquid crystals (LC) confined in a dual-beam optical trap. Droplets were created in microfluidic chips or by shaking. Optically trapped microdroplets emulsified in water and stained with fluorescent dye act as an active ultrahigh-Q optical resonant cavity hosting whispering gallery modes (WGMs). Tuning of the laser emission wavelength was achieved by a controlled deformation of the droplet shape using light-induced forces generated by dual-beam optical trap and by thermal changing of the order in the LC.

Intense excitation source of blue-green laser

NASA Astrophysics Data System (ADS)

Han, K. S.

1985-10-01

An intense and efficient excitation source for blue-green lasers useful for the space-based satellite laser applications, underwater strategic communication, and measurement of ocean bottom profile is being developed. The source in use, hypocycloidal pinch plasma (HCP), and a newly designed dense-plasma focus (DPF) can produce intense UV photons (200 to 300 nm) which match the absorption spectra of both near UV and blue green dye lasers (300 to 400 nm). During the current project period, the successful enhancement of blue-green laser output of both Coumarin 503 and LD490 dye through the spectral conversion of the HCP pumping light has been achieved with a converter dye BBQ. The factor of enhancement in the blue-green laser output energy of both Coumarin 503 and LD490 is almost 73%. This enhancement will definitely be helpful in achieving the direct high power blue-green laser (> 1 MW) with the existing blue green dye laser. On the other hand the dense-plasma focus (DPF) with new optical coupling has been designed and constructed. For the optimization of the DPF device as the UV pumping light source, the velocity of current sheath and the formation of plasma focus have been measured as function of argon or argon-deuterium fill gas pressure. Finally, the blue-green dye laser (LD490) has been pumped with the DPF device for preliminary tests. Experimental results with the DPF device show that the velocity of the current sheath follows the inverse relation of sq st. of pressure as expected. The blue-green dye (LD490) laser output exceeded 3.1 m at the best cavity tuning of laser system. This corresponds to 3J/1 cu cm laser energy extraction.

Simultaneous sampling technique for two spectral sources

NASA Technical Reports Server (NTRS)

Jarrett, Olin, Jr.

1987-01-01

A technique is described that uses a bundle of fiber optics to simultaneously sample a dye laser and a spectral lamp. By the use of a real-time display with this technique, the two signals can be superimposed, and the effect of any spectral adjustments can be immediately accessed. In the NASA's CARS system used for combustion diagnostics, the dye laser mixes with a simultaneously pulsed Nd:YAG laser at 532 nm to probe the vibrational levels of nitrogen. An illustration of the oscilloscopic display of the system is presented.

Pulsed optical-optical double resonnance spectroscopy of A 1Σ +g state of 7Li 2

NASA Astrophysics Data System (ADS)

Bernheim, R. A.; Gold, L. P.; Kelly, P. B.; Kittrell, C.; Veirs, D. K.

1980-02-01

Optical-optical double resonance using two nitrogen-laser pumped dye lasers has been used to make the first study of an excited 1σ +g state of 7Li 2. Preliminary molecular constants in cm -1 are Te = 29972, Y10 = 227, Y20 = -1.92, Y01 = 0390, Y02 = -4.3 × 10 -6and Y11 = -1.4 × 10 -3,with re = 0. 351 nm.

Tunable optofluidic microring laser based on a tapered hollow core microstructured optical fiber.

PubMed

Li, Zhi-Li; Zhou, Wen-Yuan; Luo, Ming-Ming; Liu, Yan-Ge; Tian, Jian-Guo

2015-04-20

A tunable optofluidic microring dye laser within a tapered hollow core microstructured optical fiber was demonstrated. The fiber core was filled with a microfluidic gain medium plug and axially pumped by a nanosecond pulse laser at 532 nm. Strong radial emission and low-threshold lasing (16 nJ/pulse) were achieved. Lasing was achieved around the surface of the microfluidic plug. Laser emission was tuned by changing the liquid surface location along the tapered fiber. The possibility of developing a tunable laser within the tapered simplified hollow core microstructured optical fiber presents opportunities for developing liquid surface position sensors and biomedical analysis.

Conversion of the luminescence of laser dyes in opal matrices to stimulated emission

DOE Office of Scientific and Technical Information (OSTI.GOV)

Alimov, O K; Basiev, T T; Orlovskii, Yu V

The luminescence and laser characteristics of a synthetic opal matrix filled with organic dyes are studied upon excitation by nanosecond laser pulses. The appearance of stimulated emission in a partially ordered scattering medium is investigated. It is shown that if the luminescence spectrum of a dye (oxazine-17) is located far outside the photonic bandgap of the opal matrix, stimulated emission along a preferential direction in the (111) plane is observed when pumping exceeds a threshold even without an external optical cavity. The stimulated emission spectrum is considerably narrower than the luminescence spectrum and consists of several narrow lines located withinmore » the dye luminescence band. If the luminescence spectrum of a dye (rhodamine 6G) overlaps with the photonic bandgap of the opal matrix, a different picture is observed. The loss of radiation in the matrix leads to the red shift of the luminescence spectrum, while the stimulated emission as in the case of oxazine-17 lies is observed within the luminescence band. (active media, lasers, and amplifiers)« less

Self-assembled dye-doped polymer microspheres as whispering gallery mode lasers

NASA Astrophysics Data System (ADS)

Chen, Xiaogang; Sun, Hongyi; Yang, Hongqin; Wu, Xiang; Xie, Shusen

2016-10-01

Microlasers based on high-Q whispering-gallery-mode (WGM) resonances are promising low-threshold laser sources for bio-sensing and imaging applications. In this talk, we demonstrate a cost effective approach to obtain size-controllable polymer microspheres, which can be served as good WGM microcavities. By injecting SU-8 solution into low-refractiveindex UV polymer, self-assembled spherical droplet with smooth surface can be created inside the elastic medium and then solidified by UV exposure. The size of the microspheres can be tuned from several to hundreds of microns. WGM Lasing has been achieved by optically pumping the dye-doped microspheres with ns lasers. Experimental results show that the microsphere lasers have high quality factors and low lasing thresholds. The self-assembled dye-doped polymer microspheres would provide an excellent platform for the micro-laser sources in on-chip biosensing and imaging systems.

Third-order nonlinear optical properties of acid green 25 dye by Z-scan method

NASA Astrophysics Data System (ADS)

Jeyaram, S.; Geethakrishnan, T.

2017-03-01

Third-order nonlinear optical (NLO) properties of aqueous solutions of an anthraquinone dye (Acid green 25 dye, color index: 61570) have been studied by Z-scan method with a 5 mW continuous wave (CW) diode laser operating at 635 nm. The nonlinear refractive index (n2) and the absorption coefficient (β) have been evaluated respectively from the closed and open aperture Z-scan data and the values of these parameters are found to increase with increase in concentration of the dye solution. The negative sign of the observed nonlinear refractive index (n2) indicates that the aqueous solution of acid green 25 dye exhibits self-defocusing type optical nonlinearity. The mechanism of the observed nonlinear absorption (NLA) and nonlinear refraction (NLR) is attributed respectively to reverse saturable absorption (RSA) and thermal nonlinear effects. The magnitudes of n2 and β are found to be of the order of 10-7 cm2/W and 10-3 cm/W respectively. With these experimental results, the authors suggest that acid green 25 dye may have potential applications in nonlinear optics.

Pulsed Nd-YAG laser in endodontics

NASA Astrophysics Data System (ADS)

Ragot-Roy, Brigitte; Severin, Claude; Maquin, Michel

1994-12-01

The purpose of this study was to establish an operative method in endodontics. The effect of a pulsed Nd:YAG laser on root canal dentin has been examined with a scanning electron microscope. Our first experimentation was to observe the impacts carried out perpendicularly to root canal surface with a 200 micrometers fiber optic in the presence of dye. Secondarily, the optical fiber was used as an endodontic instrument with black dye. The irradiation was performed after root canal preparation (15/100 file or 40/100 file) or directly into the canal. Adverse effects are observed. The results show that laser irradiation on root canal dentin surfaces induces a nonhomogeneous modified dentin layer, melted and resolidified dentin closed partially dentinal tubules. The removal of debris is not efficient enough. The laser treatment seems to be indicated only for endodontic and periapical spaces sterilization after conventional root canal preparation.

Three-dimensional dynamics of temperature fields in phantoms and biotissue under IR laser photothermal therapy using gold nanoparticles and ICG dye

NASA Astrophysics Data System (ADS)

Akchurin, Georgy G.; Garif, Akchurin G.; Maksimova, Irina L.; Skaptsov, Alexander A.; Terentyuk, Georgy S.; Khlebtsov, Boris N.; Khlebtsov, Nikolai G.; Tuchin, Valery V.

2010-02-01

We describe applications of silica (core)/gold (shell) nanoparticles and ICG dye to photothermal treatment of phantoms, biotissue and spontaneous tumor of cats and dogs. The laser irradiation parameters were optimized by preliminary experiments with laboratory rats. Three dimensional dynamics of temperature fields in tissue and solution samples was measured with a thermal imaging system. It is shown that the temperature in the volume region of nanoparticles localization can substantially exceed the surface temperature recorded by the thermal imaging system. We have demonstrated effective optical destruction of cancer cells by local injection of plasmon-resonant gold nanoshells and ICG dye followed by continuous wave (CW) diode laser irradiation at wavelength 808 nm.

Fluorescence lifetime imaging with near-infrared dyes

NASA Astrophysics Data System (ADS)

Becker, Wolfgang; Shcheslavskiy, Vladislav

2013-02-01

Near-infrared (NIR) dyes are used as fluorescence markers in small-animal imaging and in diffuse optical tomography of the human brain. In these applications it is important to know whether the dyes bind to proteins or other tissue constituents, and whether their fluorescence lifetimes depend on the targets they are bound to. Unfortunately, neither the lasers nor the detectors of commonly used confocal and multiphoton laser scanning microscopes allow for excitation and detection of NIR fluorescence. We therefore upgraded existing confocal TCSPC FLIM systems with NIR lasers and NIR sensitive detectors. In multiphoton systems we used the Ti:Sa laser as a one-photon excitation source in combination with an NIR-sensitive detector in the confocal beam path. We tested a number of NIR dyes in biological tissue. Some of them showed clear lifetime changes depending on the tissue structures they are bound to. We therefore believe that NIR FLIM can deliver supplementary information on the tissue constitution and on local biochemical parameters.

Construction of a Visible Diode Laser Source for Free Radical Photochemistry and Spectroscopy Experiments

NASA Technical Reports Server (NTRS)

Newman, Bronjelyn; Halpern, Joshua B.

1997-01-01

Tunable diode lasers are reliable sources of narrow-band light and comparatively cheap. Optical feedback simplifies frequency tuning of the laser diodes. We are building an inexpensive diode laser system incorporating optical feedback from a diffraction grating. The external optical cavity can be used with lasers that emit between 2 and 100 mW, and will also work if they are pulsed, although this will significantly degrade the bandwidth. The diode laser output power and bandwidth are comparable to CW dye lasers used in kinetics and dynamics experiments. However, their cost and maintenance will be much less as will alignment time. We intend to use the diode lasers to investigate CN and C2 kinetics as well as to study dissociation dynamics of atmospherically important molecules.

Apparatus and method for measurement of weak optical absorptions by thermally induced laser pulsing

DOEpatents

Cremers, D.A.; Keller, R.A.

1982-06-08

The thermal lensing phenomenon is used as the basis for measurement of weak optical absorptions when a cell containing the sample to be investigated is inserted into a normally continuous-wave operation laser-pumped dye laser cavity for which the output coupler is deliberately tilted relative to intracavity circulating laser light, and pulsed laser output ensues, the pulsewidth of which can be rlated to the sample absorptivity by a simple algorithm or calibration curve. A minimum detection limit of less than 10/sup -5/ cm/sup -1/ has been demonstrated using this technique.

Apparatus and method for measurement of weak optical absorptions by thermally induced laser pulsing

DOEpatents

Cremers, D.A.; Keller, R.A.

1985-10-01

The thermal lensing phenomenon is used as the basis for measurement of weak optical absorptions when a cell containing the sample to be investigated is inserted into a normally continuous-wave operation laser-pumped dye laser cavity for which the output coupler is deliberately tilted relative to intracavity circulating laser light, and pulsed laser output ensues, the pulsewidth of which can be related to the sample absorptivity by a simple algorithm or calibration curve. A minimum detection limit of less than 10[sup [minus]5] cm[sup [minus]1] has been demonstrated using this technique. 6 figs.

Apparatus and method for measurement of weak optical absorptions by thermally induced laser pulsing

DOEpatents

Cremers, David A.; Keller, Richard A.

1985-01-01

The thermal lensing phenomenon is used as the basis for measurement of weak optical absorptions when a cell containing the sample to be investigated is inserted into a normally continuous-wave operation laser-pumped dye laser cavity for which the output coupler is deliberately tilted relative to intracavity circulating laser light, and pulsed laser output ensues, the pulsewidth of which can be related to the sample absorptivity by a simple algorithm or calibration curve. A minimum detection limit of less than 10.sup.-5 cm.sup.-1 has been demonstrated using this technique.

An Ambipolar BODIPY Derivative for a White Exciplex OLED and Cholesteric Liquid Crystal Laser toward Multifunctional Devices.

PubMed

Chapran, Marian; Angioni, Enrico; Findlay, Neil J; Breig, Benjamin; Cherpak, Vladyslav; Stakhira, Pavlo; Tuttle, Tell; Volyniuk, Dmytro; Grazulevicius, Juozas V; Nastishin, Yuriy A; Lavrentovich, Oleg D; Skabara, Peter J

2017-02-08

A new interface engineering method is demonstrated for the preparation of an efficient white organic light-emitting diode (WOLED) by embedding an ultrathin layer of the novel ambipolar red emissive compound 4,4-difluoro-2,6-di(4-hexylthiopen-2-yl)-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (bThBODIPY) in the exciplex formation region. The compound shows a hole and electron mobility of 3.3 × 10 -4 and 2 × 10 -4 cm 2 V -1 s -1 , respectively, at electric fields higher than 5.3 × 10 5 V cm -1 . The resulting WOLED exhibited a maximum luminance of 6579 cd m -2 with CIE 1931 color coordinates (0.39; 0.35). The bThBODIPY dye is also demonstrated to be an effective laser dye for a cholesteric liquid crystal (ChLC) laser. New construction of the ChLC laser, by which a flat capillary with an optically isotropic dye solution is sandwiched between two dye-free ChLC cells, provides photonic lasing at a wavelength well matched with that of a dye-doped planar ChLC cell.

Physicochemical and Nonlinear Optical Properties of Novel Environmentally Benign Heterocyclic Azomethine Dyes: Experimental and Theoretical Studies

PubMed Central

Afzal, S. M.; Razvi, M. A. N.; Khan, Salman A.; Osman, Osman I.; Bakry, Ahmed H.; Asiri, Abdullah M.

2016-01-01

Novel heterocyclic azomethine dyes were prepared by the reaction of anthracene-9-carbaldehyde with different heterocyclic amines under microwave irradiation. Structures of the azomethine dyes were confirmed by the elemental analysis, mass spectrometry and several spectroscopic techniques. We studied absorbance and fluorescence spectra of the azomethine dyes in various solvents. They are found to be good absorbers and emitters. We also report photophysical properties like, extinction coefficient, oscillator strength, stokes shift and transition dipole moment. This reflects physicochemical behaviors of synthesized dyes. In addition, their intramolecular charge transfer and nonlinear optical properties, supported by natural bond orbital technique, were also studied computationally by density functional theory. The negative nonlinear refractive index and nonlinear absorption coefficient were measured for these dyes using the closed and open aperture Z-scan technique with a continuous wave helium-neon laser. These are found to vary linearly with solution concentration. PMID:27631371

From Dye Laser Factory to Portable Semiconductor Laser: Four Generations of Sodium Guide Star Lasers for Adaptive Optics in Astronomy and Space Situational Awareness

NASA Astrophysics Data System (ADS)

d'Orgeville, C.; Fetzer, G.

This presentation recalls the history of sodium guide star laser systems used in astronomy and space situational awareness adaptive optics, analysing the impact that sodium laser technology evolution has had on routine telescope operations. While it would not be practical to describe every single sodium guide star laser system developed to date, it is possible to characterize their evolution in broad technology terms. The first generation of sodium lasers used dye laser technology to create the first sodium laser guide stars in Hawaii, California, and Spain in the late 1980's and 1990's. These experimental systems were turned into the first laser guide star facilities to equip medium-to-large diameter adaptive optics telescopes, opening a new era of LGS AO-enabled diffraction-limited imaging from the ground. Although they produced exciting scientific results, these laser guide star facilities were large, power-hungry and messy. In the USA, a second-generation of sodium lasers was developed in the 2000's that used cleaner, yet still large and complex, solid-state laser technology. These are the systems in routine operation at the 8-10m class astronomical telescopes and 4m-class satellite imaging facilities today. Meanwhile in Europe, a third generation of sodium lasers was being developed using inherently compact and efficient fiber laser technology, and resulting in the only commercially available sodium guide star laser system to date. Fiber-based sodium lasers will be deployed at two astronomical telescopes and at least one space debris tracking station this year. Although highly promising, these systems remain significantly expensive and they have yet to demonstrate high performance in the field. We are proposing to develop a fourth generation of sodium lasers: based on semiconductor technology, these lasers could provide the final solution to the problem of sodium laser guide star adaptive optics for all astronomy and space situational awareness applications.

Raman-Induced Kerr Effect Studies.

DTIC Science & Technology

1980-06-10

appropriate parameters. He also has access to a ten joule Nd: glass laser a part of whose beam pumps a tunable dye laser. These lasers, according to theor...introduced. Quantum limited signal- proportional to the spontaneous Raman cross section. The to.noise ratios may be approached by the use of optical...ratio result from the use of optical ={B(W3 - w2) - 2A(t, - w2)1/24. (3) In (2) and (3), A(Aw) and BCAw) are the complex nuclear Manuscript received

Recent advance to 3 × 10(-5) rad near diffraction-limited beam divergence of dye laser with transverse-discharge flash-lamp pumping.

PubMed

Trusov, K K

1994-02-20

A new experimental setup of a Rhodamine 6G dye laser with a transverse-discharge flash-lamp-pumping system is presented. It differs from a previous setup [Sov. J. Quantum Electron. 16, 468-471 (1989)] in that it has a larger laser beam aperture (32 mm) and higher pumping energy (1 kJ), which made it possible to test the scalability and reach near diffraction-limited laser beam divergence of 3 × 10(-5) rad FWHM at beam energy 1.4 J. The effect of spectral dispersion in the active medium and of other optical elements on the beam divergence is also discussed.

DEMONSTRATION BULLETIN: RAPID OPTICAL SCREEN TOOL (ROST™) - LORAL CORPORATION

EPA Science Inventory

The Loral Rapid Optical Screen Tool (ROST™) is a tunable dye laser system used for the detection of petroleum, semi-volatile, and some volatile organic compounds in soils. The technology is used in conjunction with a cone penetrometer (CP).

Comparison of the photothermal effects of 808nm gold nanorod and indocyanine green solutions using an 805nm diode laser

NASA Astrophysics Data System (ADS)

Hasanjee, Aamr M.; Zhou, Feifan; West, Connor; Silk, Kegan; Doughty, Austin; Bahavar, Cody F.; Chen, Wei R.

2016-03-01

Non-invasive laser immunotherapy (NLIT) is a treatment method for metastatic cancer which combines noninvasive laser irradiation with immunologically modified nanostructures to ablate a primary tumor and induce a systemic anti-tumor response. To further expand the development of NLIT, two different photosensitizing agents were compared: gold nanorods (GNR) with an optical absorption peak of 808 nm and indocyanine green (ICG) with an optical absorption peak of ~800 nm. Various concentrations of GNR and ICG solutions were irradiated at different power densities using an 805 nm diode laser, and the temperature of the solutions was monitored during irradiation using a thermal camera. For comparison, dye balls made up of a 1:1 volume ratio of gel solution to GNR or ICG solution were placed in phantom gels and were then irradiated using the 805 nm diode laser to imitate the effect of laser irradiation on in vivo tumors. Non-invasive laser irradiation of GNR solution for 2 minutes resulted in a maximum increase in temperature by 31.8 °C. Additionally, similar irradiation of GNR solution dye ball within phantom gel for 10 minutes resulted in a maximum temperature increase of 8.2 °C. Comparatively, non-invasive laser irradiation of ICG solution for 2 minutes resulted in a maximum increase in temperature by 28.0 °C. Similar irradiation of ICG solution dye ball within phantom gel for 10 minutes yielded a maximum temperature increase of only 3.4 °C. Qualitatively, these studies showed that GNR solutions are more effective photosensitizing agents than ICG solution.

Laser-Activated Shape Memory Polymer Microactuator for Thrombus Removal Following Ischemic Stroke: Preliminary In Vitro Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Small, W; Metzger, M F; Wilson, T S

2004-09-23

Due to the narrow (3-hour) treatment window for effective use of the thrombolytic drug recombinant tissue-type plasminogen activator (rt-PA), there is a need to develop alternative treatments for ischemic stroke. We are developing an intravascular device for mechanical thrombus removal using shape memory polymer (SMP). We propose to deliver the SMP microactuator in its secondary straight rod form (length = 4 cm, diameter = 350 {micro}m) through a catheter distal to the vascular occlusion. The microactuator, which is mounted on the end of an optical fiber, is then transformed into its primary corkscrew shape by laser heating (diode laser, {lambda}more » = 800 nm) above its soft phase glass transition temperature (T{sub gs} = 55 C). Once deployed, the microactuator is retracted and the captured thrombus is removed to restore blood flow. The SMP is doped with indocyanine green (ICG) dye to increase absorption of the laser light. Successful deployment of the microactuator depends on the optical properties of the ICG-doped SMP and the optical coupling efficiency of the interface between the optical fiber and the SMP. Spectrophotometry, thermal imaging, and computer simulation aided the initial design effort and continue to be useful tools for optimization of the dye concentration and laser power. Thermomechanical testing was performed to characterize the elastic modulus of the SMP. We have demonstrated laser-activation of the SMP microactuator in air at room temperature, suggesting this concept is a promising therapeutic alternative to rt-PA.« less

Tailoring of optical properties of fluorescein using green synthesized gold nanoparticles.

PubMed

John, Jisha; Thomas, Lincy; George, Nibu A; Kurian, Achamma; George, Sajan D

2015-06-28

Dye-nanoparticle mixtures hold great promise in biological as well as photonics applications due to their capability to tailor the emission behavior of dye by tuning the nanoparticles parameters. However, as compared to the well-defined dye-nanoparticle distance, studies lack the understanding of homogenous mixtures of dye and nanoparticles. In this work, we investigate the influence of shape and concentration of gold nanoparticles prepared via green synthesis on the optical properties of fluorescein dye in a dye-nanoparticle mixture. We have investigated the radiative path of deexcitation using steady state fluorescence and the non-radiative path is probed using a laser based dual-beam thermal lens technique. The energy transfer efficiency as well as dye-nanoparticle distance is studied using both techniques. Furthermore, we have explored the influence of nanoparticles parameters on the fluorescence quantum yield of fluorescein using the thermal lens technique. The studies indicate that spherical nanoparticles are efficient quenchers while star shaped nanoparticles can probe larger dye-NP distances. The tailoring of dye properties by tuning nanoparticle parameters can be utilized in diverse areas including bioimaging, solar cells, and sensors.

Fiber-Type Random Laser Based on a Cylindrical Waveguide with a Disordered Cladding Layer.

PubMed

Zhang, Wei Li; Zheng, Meng Ya; Ma, Rui; Gong, Chao Yang; Yang, Zhao Ji; Peng, Gang Ding; Rao, Yun Jiang

2016-05-25

This letter reports a fiber-type random laser (RL) which is made from a capillary coated with a disordered layer at its internal surface and filled with a gain (laser dye) solution in the core region. This fiber-type optical structure, with the disordered layer providing randomly scattered light into the gain region and the cylindrical waveguide providing confinement of light, assists the formation of random lasing modes and enables a flexible and efficient way of making random lasers. We found that the RL is sensitive to laser dye concentration in the core region and there exists a fine exponential relationship between the lasing intensity and particle concentration in the gain solution. The proposed structure could be a fine platform of realizing random lasing and random lasing based sensing.

Shape memory polymer (SMP) gripper with a release sensing system

DOEpatents

Maitland, Duncan J.; Lee, Abraham P.; Schumann, Daniel L.; Silva, Luiz Da

2000-01-01

A system for releasing a target material, such as an embolic coil from an SMP located at the end of a catheter utilizing an optical arrangement for releasing the material. The system includes a laser, laser driver, display panel, photodetector, fiber optics coupler, fiber optics and connectors, a catheter, and an SMP-based gripper, and includes a release sensing and feedback arrangement. The SMP-based gripper is heated via laser light through an optic fiber causing the gripper to release a target material (e.g., embolic coil for therapeutic treatment of aneurysms). Various embodiments are provided for coupling the laser light into the SMP, which includes specific positioning of the coils, removal of the fiber cladding adjacent the coil, a metal coating on the SMP, doping the SMP with a gradient absorbing dye, tapering the fiber optic end, coating the SMP with low refractive index material, and locating an insert between the fiber optic and the coil.

Multicolored Emission and Lasing in DCM-Adamantane Plasma Nanocomposite Optical Films.

PubMed

Alcaire, María; Cerdán, Luis; Zamarro, Fernando Lahoz; Aparicio, Francisco J; González, Juan Carlos; Ferrer, Francisco J; Borras, Ana; Espinós, Juan Pedro; Barranco, Angel

2017-03-15

We present a low-temperature versatile protocol for the fabrication of plasma nanocomposite thin films to act as tunable emitters and optical gain media. The films are obtained by the remote plasma-assisted deposition of a 4-(dicyano-methylene)-2-methyl-6-(4-dimethylamino-styryl)-4H-pyran (DCM) laser dye alongside adamantane. The experimental parameters that determine the concentration of the dye in the films and their optical properties, including light absorption, the refractive index, and luminescence, are evaluated. Amplified spontaneous emission experiments in the DCM/adamantane nanocomposite waveguides show the improvement of the copolymerized nanocomposites' properties compared to films that were deposited with DCM as the sole precursor. Moreover, one-dimensional distributed feed-back laser emission is demonstrated and characterized in some of the nanocomposite films that are studied. These results open new paths for the optimization of the optical and lasing properties of plasma nanocomposite polymers, which can be straightforwardly integrated as active components in optoelectronic devices.

Hollow optical fiber induced solar cells with optical energy storage and conversion.

PubMed

Ding, Jie; Zhao, Yuanyuan; Duan, Jialong; Duan, Yanyan; Tang, Qunwei

2017-11-09

Hollow optical fiber induced dye-sensitized solar cells are made by twisting Ti wire/N719-TiO 2 nanotube photoanodes and Ti wire/Pt (CoSe, Pt 3 Ni) counter electrodes, yielding a maximized efficiency of 0.7% and good stability. Arising from optical energy storage ability, the solar cells can generate electricity without laser illumination.

Peculiarities of the statistics of spectrally selected fluorescence radiation in laser-pumped dye-doped random media

NASA Astrophysics Data System (ADS)

Yuvchenko, S. A.; Ushakova, E. V.; Pavlova, M. V.; Alonova, M. V.; Zimnyakov, D. A.

2018-04-01

We consider the practical realization of a new optical probe method of the random media which is defined as the reference-free path length interferometry with the intensity moments analysis. A peculiarity in the statistics of the spectrally selected fluorescence radiation in laser-pumped dye-doped random medium is discussed. Previously established correlations between the second- and the third-order moments of the intensity fluctuations in the random interference patterns, the coherence function of the probe radiation, and the path difference probability density for the interfering partial waves in the medium are confirmed. The correlations were verified using the statistical analysis of the spectrally selected fluorescence radiation emitted by a laser-pumped dye-doped random medium. Water solution of Rhodamine 6G was applied as the doping fluorescent agent for the ensembles of the densely packed silica grains, which were pumped by the 532 nm radiation of a solid state laser. The spectrum of the mean path length for a random medium was reconstructed.

Optoelectronic and photoacoustic studies of an organic dye synthesized through green route

NASA Astrophysics Data System (ADS)

Vijayakumar, S.; Sreelatha, S.; Hatamimoslehabadi, M.; Yelleswarappu, C. S.

2017-10-01

An azo dye was prepared through an environmentally benign and economically feasible synthesis route with cardanol as a starting material. Cardanol is a cost-effective and renewable natural source obtained from Cashew Nut Shell Liquid, a by-product of the cashew industry. The dye was spectrally characterized by IR, UV-Vis, NMR and fluorescence studies. UV-Vis absorption showed a bathochromic shift between solvents of lower and higher polarities. Nonlinear optical and photoacoustic properties were studied using a frequency doubled Nd:YAG laser producing 532 nm laser pulses of 3 ns pulse width. Results show that the nonlinear absorption coefficient decreases with the increase of on-axis intensity, suggesting excited state absorption as the principal mechanism. The observed nonlinearity has applications in optoelectronics.

High-speed photography during laser-based gall bladder stone lithotripsy

NASA Astrophysics Data System (ADS)

Kokaj, Jahja O.

2001-04-01

Shadowgraphy of gall bladder stone, which is held by a basket and immersed in a civete is performed. The exposure time is determined by the time of a N-Dye laser pulse used as a lightening source for photography. The shadowgram is projected in the objective of a camera which is connected to a microscope. The light coming from the laser, illuminates the civete collecting optical information of the stone and physical phenomena appearing above the stone. On top of the stone a tip of optical fiber is fixed, which is used for transmitting Ho:Yag laser power to the stone. Using a computer and time delay the laser pulses used for destruction and illumination are synchronized. Since the N-Dye laser pulse is pico-second range and the Ho:Yag laser pulse is in the range of micro-second, many image frames are obtained within the time of one pulse applied during the destruction. It is known that in the process of stone destruction several phenomena like plume, plasma, shock wave and bubble formation take place. However, the physical mechanism of the stone destruction is not yet completely understood. From the obtained results the above phenomena are studied which gives new information and clue for understanding some of the mentioned phenomena. The laser power which is guided by an optical fiber into the gall bladder or kidney of the human body can damage the living tissue and cause some serious health problems. For this reason the fiber needs to be oriented properly during the action of the laser power.

Observation of sub-100-fs optical response from spin-coated films of squarylium dye J aggregates

NASA Astrophysics Data System (ADS)

Furuki, Makoto; Tian, Minquan; Sato, Yasuhiro; Pu, Lyong Sun; Kawashima, Hitoshi; Tatsuura, Satoshi; Wada, Osamu

2001-04-01

For spin-coated films of squarylium dye J aggregates, ultrafast nonlinear optical responses were investigated by pump-probe measurements. By using a broadband mode-locked titanium:sapphire laser, we succeeded in observing the optical response with a time resolution of better than 60 fs. Time-resolved transmission data are shown for different excitation wavelengths, resonant to the excitonic absorption band and off-resonant. Relaxation times of the absorption saturation were evaluated to be 140 fs (fast component) and 950 fs (slow component) in the case of resonant excitation and 98 fs in the case of off-resonant excitation.

Single bead near-infrared random laser based on silica-gel infiltrated with Rhodamine 640

NASA Astrophysics Data System (ADS)

Moura, André L.; Barbosa-Silva, Renato; Dominguez, Christian T.; Pecoraro, Édison; Gomes, Anderson S. L.; de Araújo, Cid B.

2018-04-01

Photoluminescence properties of single bead silica-gel (SG) embedded with a laser-dye were studied aiming at the operation of near-infrared (NIR) Random Lasers (RLs). The operation of RLs in the NIR spectral region is especially important for biological applications since the optical radiation has deep tissue penetration with negligible damage. Since laser-dyes operating in the NIR have poor stability and are poor emitters, ethanol solutions of Rhodamine 640 (Rh640) infiltrated in SG beads were used. The Rh640 concentrations in ethanol varied from 10-5 to 10-2 M and the excitation at 532 nm was made by using a 7 ns pulsed laser. The proof-of-principle RL scheme herein presented was adopted in order to protect the dye-molecules from the environment and to favor formation of aggregates. The RL emission from ≈650 nm to 720 nm, beyond the typical Rh640 monomer and dimer wavelengths emissions range, was attributed to the trade-off between reabsorption and reemission processes along the light pathways inside the SG bead and the contribution of Rh640 aggregates.

Spectroscopy and laser action of the "red perylimide dye" in various solvents

NASA Astrophysics Data System (ADS)

Gvishi, R.; Reisfeld, R.; Burshtein, Z.

1993-10-01

Optical properties of the red perylimide laser dye in various solvents are studied. The absorption spectrum exhibits two main bands, in the ranges 480-600 and 400-460 nm, due to the S 0-S 1 and S 0-S 2 transition. The fluorescence spectrum is a mirror image of the S 0-S 1 absorption (shift of ˜ 30-50 nm). The Stokes shift increases with solvent polarity. Such dye-solvent interactions are compared to theoretical predictions. The fluorescence quantum yields approaches unity in all the solvents studied. Laser tunability around 30 nm was obtained each time, covering the spectral range 580-640 nm. This interval is important for medical applications in photodynamic therapy and fluorescence diagnostics. The laser threshold energy varied from 0.35 mJ/pulse in cyclohexane to 1.87 mJ/pulse in methanol, and the slope efficiency from about 6.6% in methanol to 14% in xylenes. The laser output was stable for several hours of operation under an average pump energy of about 20 mJ/pulse at 1 Hz repetition rate, without flow.

Simultaneous RGB lasing from a single-chip polymer device.

PubMed

Yamashita, Kenichi; Takeuchi, Nobutaka; Oe, Kunishige; Yanagi, Hisao

2010-07-15

This Letter describes the fabrication and operation of a single-chip white-laser device. The laser device has a multilayered structure consisting of three laser layers. Each laser layer comprises polymer claddings and a waveguide core doped with organic dye. In each laser layer, grating corrugations were fabricated by UV-nanoimprint lithography that act as distributed-feedback cavity structures. Under optical pumping, lasing output with red, green, and blue colors was simultaneously obtained from the sample edge.

Measuring evaporation rates of laser-trapped droplets by use of fluorescent morphology-dependent resonances.

PubMed

Pastel, R; Struthers, A

2001-05-20

Morphology-dependent resonances (MDRs) are used to measure accurately the evaporation rates of laser-trapped 1- to 2-mum droplets of ethylene glycol. Droplets containing 3 x 10(-5) M Rhodamine-590 laser dye are optically trapped in a 20-mum hollow fiber by two counterpropagating 150-mW, 800-nm laser beams. A weaker 532-nm laser excites the dye, and fluorescence emission is observed near 560 nm as the droplet evaporates. A complete series of first-order TE and TM MDRs dominates the fluorescent output. MDR mode identification sizes the droplets and provides accurate evaporation rates. We verify the automated MDR mode identification by counting fringes in a videotape of the experiment. The longitudinal spring constant of the trap, measured by analysis of the videotaped motion of droplets perturbed from the trap center, provides independent verification of the laser's intensity within the trap.

Measuring Evaporation Rates of Laser-Trapped Droplets by Use of Fluorescent Morphology-Dependent Resonances

NASA Astrophysics Data System (ADS)

Pastel, Robert; Struthers, Allan

2001-05-01

Morphology-dependent resonances (MDRs) are used to measure accurately the evaporation rates of laser-trapped 1- to 2- m droplets of ethylene glycol. Droplets containing 3 x10-5 M Rhodamine-590 laser dye are optically trapped in a 20- m hollow fiber by two counterpropagating 150-mW, 800-nm laser beams. A weaker 532-nm laser excites the dye, and fluorescence emission is observed near 560 nm as the droplet evaporates. A complete series of first-order TE and TM MDRs dominates the fluorescent output. MDR mode identification sizes the droplets and provides accurate evaporation rates. We verify the automated MDR mode identification by counting fringes in a videotape of the experiment. The longitudinal spring constant of the trap, measured by analysis of the videotaped motion of droplets perturbed from the trap center, provides independent verification of the laser s intensity within the trap.

NaK 22Σ+ → 11Σ+ band optically pumped laser near 1.02 μm

NASA Astrophysics Data System (ADS)

Clark, B. K.; Luh, W. T.; Huennekens, J.

1989-08-01

Optically pumped laser emission has been observed on the NaK 2( A)1Σ+ → 1( X)1Σ+ electronic state transition. The emission occurs between 1.015 and 1.035 μm when a sodium-potassium heat-pipe oven is pumped with 695 745 nm pulsed dye laser radiation. The laser emission occurs on many ro-vibrational transitions without the use of cavity mirrors. However, the addition of a simple cavity increases both the number of observed lasing transitions and the amplitude of the emission on each line. We report our results for the dependence of the emission intensity on pump laser power, oven temperature, and buffer gas pressure.

Electrically tunable laser based on heliconical cholesteric (Conference Presentation)

NASA Astrophysics Data System (ADS)

Xiang, Jie; Varanytsia, Andrii; Minkowski, Fred; Paterson, Daniel A.; Imrie, Corrie T.; Lavrentovich, Oleg D.; Palffy-Muhoray, Peter

2016-09-01

STUDENT CONTRIBUTION: Cholesteric liquid crystals (CLC) self-assemble into a periodic supramolecular helical structure with properties of a one-dimensional photonic crystal. The CLCs doped with a fluorescent dye and optical pump enable a distributed feedback cavity and lasing [1]. Although lasing was observed in range of wavelength from near UV to near IR, a practical method of tuning of emission wavelength from a dye-doped CLC without structural destruction of a helix is not demonstrated yet. In this work, we demonstrate an electrically tunable dye-doped CLC laser based on the so-called oblique helicoidal, or heliconical, CLC state [2,3]. In this state, the molecules twist around the helicoidal axis, making an angle smaller than 90 degrees with the axis. Molecular tilt makes the heliconical structure different from the regular CLC (in which the molecules are perpendicular to the axis) and enable electric tunability [2,3]. An electric field applied parallel to the heliconical axis changes the pitch but does not realign the axis. When the field increases, the pitch decreases. As a result, the selective reflection band and a lasing wavelength move towards shorter wavelength. Using heliconical CLC and two laser dyes DCM and LD688, we demonstrate effective tuning of the laser emission wavelength from 574 nm to 722 nm. With appropriate laser dyes, the spectrum can be extended from near UV to near IR. Efficient electric tuning in the broad spectral range and small size of the heliconical cholesteric lasers makes them potentially useful for optical and biomedical applications. [1] P. Palffy-Muhoay, W.Y. Cao, M. Moreira, B. Taheri, A. Munoz, Photonics and lasing in liquid crystal [2] J. Xiang, S.V. Shiyanovskii, C.T. Imrie, O.D. Lavrentovich, Electrooptic Response of Chiral Nematic Liquid Crystals with Oblique Helicoidal Director, Phys Rev Lett, 112 (2014) 217801. [3] J. Xiang, Y.N. Li, Q. Li, D.A. Paterson, J.M.D. Storey, C.T. Imrie, O.D. Lavrentovich, Electrically Tunable Selective Reflection of Light from Ultraviolet to Visible and Infrared by Heliconical Cholesterics, Adv Mater, 27 (2015) 3014-3018.

Dye-enhanced laser welding for skin closure.

PubMed

DeCoste, S D; Farinelli, W; Flotte, T; Anderson, R R

1992-01-01

The use of a laser to weld tissue in combination with a topical photosensitizing dye permits selective delivery of energy to the target tissue. A combination of indocyanine green (IG), absorption peak 780 nm, and the near-infrared (IR) alexandrite laser was studied with albino guinea pig skin. IG was shown to bind to the outer 25 microns of guinea pig dermis and appeared to be bound to collagen. The optical transmittance of full-thickness guinea pig skin in the near IR was 40% indicating that the alexandrite laser should provide adequate tissue penetration. Laser "welding" of skin in vivo was achieved at various concentrations of IG from 0.03 to 3 mg/cc using the alexandrite at 780 nm, 250-microseconds pulse duration, 8 Hz, and a 4-mm spot size. A spectrum of welds was obtained from 1- to 20-W/cm2 average irradiance. Weak welds occurred with no thermal damage obtained at lower irradiances: stronger welds with thermal damage confined to the weld site occurred at higher irradiances. At still higher irradiances, local vaporization occurred with failure to "weld." Thus, there was an optimal range of irradiances for "welding," which varied inversely with dye concentration. Histology confirmed the thermal damage results that were evident clinically. IG dye-enhanced laser welding is possible in skin and with further optimization may have practical application.

Laser-initiated decomposition products of indocyanine green (ICG) and carbon black sensitized biological tissues

NASA Astrophysics Data System (ADS)

Kokosa, John M.; Przyjazny, Andrzej; Bartels, Kenneth E.; Motamedi, Massoud; Hayes, Donald J.; Wallace, David B.; Frederickson, Christopher J.

1997-05-01

Organic dyes have found increasing use a s sensitizers in laser surgical procedures, due to their high optical absorbances. Little is known, however, about the nature of the degradation products formed when these dyes are irradiated with a laser. Previous work in our laboratories has shown that irradiation of polymeric and biological tissues with CO2 and Nd:YAG lasers produces a host of volatile and semivolatile by-products, some of which are known to be potential carcinogens. This work focuses on the identification of the chemical by-products formed by diode laser and Nd:YAG laser irradiation of indocyanine green (ICG) and carbon black based ink sensitized tissues, including bone, tendon and sheep's teeth. Samples were mounted in a 0.5-L Pyrex sample chamber equipped with quartz optical windows, charcoal filtered air inlet and an outlet attached to an appropriate sample trap and a constant flow pump. By-products were analyzed by GC/MS and HPLC. Volatiles identified included benzene and formaldehyde. Semi-volatiles included traces of polycyclic aromatics, arising from the biological matrix and inks, as well as fragments of ICG and the carbon ink components. The significance of these results will be discussed, including the necessity of using appropriate evacuation devices when utilizing lasers for surgical procedures.

Comparison of microleakages of photo-cured composites using three different light sources: halogen lamp, LED and argon laser: an in vitro study.

PubMed

Tielemans, M; Compere, Ph; Geerts, S O; Lamy, M; Limme, M; De Moor, R J G; Delmé, K I M; Bertrand, M F; Rompen, E; Nammour, S

2009-01-01

In this study, we compared the microleakage of composite fillings cured with halogen bulb, LED and argon ion laser (488 nm). Twenty-four extracted human molars were divided randomly in three groups. Six cavities were prepared on the coronal part of each tooth. Standard cavities (1.7 x 2 mm) were prepared. Cavities were acid etched, sealed with Scotch Bond 1 and filled by a hybrid composite. Cavities were exposed to one light source, thermocycled and immersed in a 2% methylene blue dye solution. Dye penetration in the leakage of cavities was recorded using a digital optical microscope. Mean values of percentage of dye penetrations in microleakages of cavities were 49.303 +/- 5.178% for cavities cured with LED, 44.486 +/- 6.075% with halogen bulb and 36.647 +/- 5.936% for those cured by argon laser. Statistically significant difference exists between cavities cured by halogen vs LED (P < 0.01), halogen vs laser (P < 0.001) and LED vs laser (P < 0.001). The lowest microleakage was observed in the cavities and composites cured with argon ion laser.

Three color laser fluorometer for studies of phytoplankton fluorescence

NASA Technical Reports Server (NTRS)

Phinney, David A.; Yentsch, C. S.; Rohrer, J.

1988-01-01

A three-color laser fluorometer has been developed for field work operations. Using two tunable dye lasers (excitation wavelengths at 440 nm and 530 nm), broadband wavelength optical filters were selected to obtain maximum fluorescence sensitivity at wavelengths greater than 675 nm (chlorophyll) and 575 + or - 15 nm (phycoerythrin). The laser fluorometer permits the measurement of phytoplankton pigments under static or flowing conditions and more closely resembles the time scales (ns) and energy levels (mW) of other laser-induced fluorescence instruments.

Resonant third-order optical nonlinearities of thin films containing J-aggregates of a cyanine dye or a squarylium dye

NASA Astrophysics Data System (ADS)

Li, Zhongyu; Jin, Zhaohui; Kasatani, Kazuo

2005-01-01

The third-order optical nonlinearities and responses of thin films containing the J-aggregates of a cyanine dye or a squarylium dye were measured using the degenerate four-wave mixing (DFWM) technique under resonant conditions. The sol-gel silica coating films containing the J-aggregates of the cyanine dye, NK-3261, are stable at room temperature and durable against laser beam irradiation. The temporal profiles of the DFWM signal were measured with a time resolution of 0.3 ps, and were found to consist of at least three components, i.e., the coherent instantaneous nonlinear response and the two slow responses with delay time constants of ca. 1.0 ps and ca. 5.6 ps. The contribution of the later was small. The electronic component of the effective third-order optical nonlinear susceptibility of the film had value of as high as ca. 3.0 x 10-7 esu. We also studied the neat film of a squarylium dye J-aggregates. The temporal profile of the DFWM signal of the neat film of squarylium dye was also found to consist of at least three components, the coherent instantaneous nonlinear response and the delayed response with decay time constants of ca. 0.6 ps and ca. 6.5 ps. The contribution of the slow tail was also very small. The electronic component of effective third-order optical nonlinear susceptibility of the neat film of squarylium dye had value of as high as ca. 3.6 x 10-8 esu.

Optically sectioned wide-field fluorescence lifetime imaging endoscopy enabled by structured illumination (Conference Presentation)

NASA Astrophysics Data System (ADS)

Hinsdale, Taylor; Malik, Bilal H.; Rico-Jimenez, Jose J.; Jo, Javier A.; Maitland, Kristen C.

2016-03-01

We present a wide-field fluorescence lifetime imaging (FLIM) system with optical sectioning by structured illumination microscopy (SIM). FLIM measurements were made using a time gated ICCD camera in conjunction with a pulsed nitrogen dye laser operating at 450 nm. Intensity images were acquired at multiple time delays from a trigger initiated by a laser pulse to create a wide-field FLIM image, which was then combined with three phase SIM to provide optical sectioning. Such a mechanism has the potential to increase the reliability and accuracy of the FLIM measurements by rejecting background intensity. SIM also provides the opportunity to create volumetric FLIM images with the incorporation of scanning mechanisms for the sample plane. We present multiple embodiments of such a system: one as a free space endoscope and the other as a fiber microendoscope enabled by the introduction of a fiber bundle. Finally, we demonstrate the efficacy of such an imaging system by imaging dyes embedded in a tissue phantom.

Broad band nonlinear optical absorption measurements of the laser dye IR26 using white light continuum Z-scan

NASA Astrophysics Data System (ADS)

Dey, Soumyodeep; Bongu, Sudhakara Reddy; Bisht, Prem Ballabh

2017-03-01

We study the nonlinear optical response of a standard dye IR26 using the Z-scan technique, but with the white light continuum. The continuum source of wavelength from 450 nm to 1650 nm has been generated from the photonic crystal fiber on pumping with 772 nm of Ti:Sapphire oscillator. The use of broadband incident pulse enables us to probe saturable absorption (SA) and reverse saturable absorption (RSA) over the large spectral range with a single Z-scan measurement. The system shows SA in the resonant region while it turns to RSA in the non-resonant regions. The low saturation intensity of the dye can be explained based on the simultaneous excitation from ground states to various higher energy levels with the help of composite energy level diagram. The cumulative effects of excited state absorption and thermal induced nonlinear optical effects are responsible for the observed RSA.

Elucidation of Free Radical and Optogalvanic Spectroscopy Associated with Microgravity Combustion via Conventional and Novel Laser Platforms

NASA Technical Reports Server (NTRS)

Misra, Prabhakar; She, Yong-Bo; Zhu, Xin-Ming; King, Michael

1997-01-01

Combustion studies under both normal gravity and microgravity conditions depend a great deal on the availability and quality of the diagnostic systems used for such investigations. Microgravity phenomena are specially susceptible to even small perturbations and therefore non-intrusive diagnostic techniques are of paramount importance for successful understanding of reduced-gravity combustion phenomena. Several non-intrusive diagnostic techniques are available for probing and delineating normal as well as reduced gravity combustion processes, such as Rayleigh scattering, Raman scattering, Mie scattering, velocimetry, interferometric and Schlieren techniques, emission and laser-induced fluorescence (LIF) spectroscopy. Our approach is to use the LIF technique as a non-intrusive diagnostic tool for the study of combustion-associated free radicals and use the concomitant optogalvanic transitions to accomplish precise calibration of the laser wavelengths used for recording the excitation spectra of transient molecular species. In attempting to perform spectroscopic measurements on chemical intermediates, we have used conventional laser sources as well as new and novel platforms employing rare-earth doped solid-state lasers. Conventional (commercially available) sources of tunable UV laser radiation are extremely cumbersome and energy-consuming devices that are not very suitable for either in-space or in-flight (or microgravity drop tower) experiments. Traditional LIF sources of tunable UV laser radiation involve in addition to a pump laser (usually a Nd:YAG laser with an attached frequency-doubling stage), a tunable dye laser. In turn, the dye laser has to be provided with a dye circulation system and a subsequent stage for frequency-doubling of the dye laser radiation, together with a servo-tuning system (termed the 'Autotracker') to follow the wavelength changes and also an optical system (called the 'Frequency Separator') for separation of the emanating visible and UV beams. In contrast to this approach, we have devised an alternate arrangement for recording LIF excitation spectra of free radicals (following appropriate precursor fragmentation) that utilizes a tunable rare-earth doped solid state laser system with direct UV pumping. We have designed a compact and portable tunable UV laser system incorporating features necessary for both in-space and in-flight spectroscopy experiments. For the purpose of LIF excitation, we have developed an all-solid-state tunable UV laser that employs direct pumping of the solid-state UV-active medium employing UV harmonics from a Nd:YAG laser. An optical scheme with counterpropagating photolysis and excitation beams focused by suitable lenses into a reaction vacuum chamber was employed.

Whispering gallery mode laser based on cholesteric liquid crystal microdroplets as temperature sensor

NASA Astrophysics Data System (ADS)

Zhao, Liyuan; Wang, Yan; Yuan, Yonggui; Liu, Yongjun; Liu, Shuangqiang; Sun, Weimin; Yang, Jun; Li, Hanyang

2017-11-01

We developed a tunable whispering gallery mode (WGM) microlaser based on dye-doped cholesteric liquid crystal (CLC) microdroplets with controllable size in an aqueous environment. An individual dye-doped CLC microdroplet confined at the tip of a microcapillary was optically pumped via a tapered optical fiber tip positioned within its vicinity. Numerical simulations and various spectral characteristics verify the WGM resonance of the lasing in microdroplets. Thermal tuning of the lasing modes is realized due to the thermo-optic effect of CLC. The proposed CLC microdroplet-based WGM resonator was applied as a temperature sensor and exhibited maximum temperature sensitivity up to 0.96 nm/°C.

Random lasing in dye-doped polymer dispersed liquid crystal film

NASA Astrophysics Data System (ADS)

Wu, Rina; Shi, Rui-xin; Wu, Xiaojiao; Wu, Jie; Dai, Qin

2016-09-01

A dye-doped polymer-dispersed liquid crystal film was designed and fabricated, and random lasing action was studied. A mixture of laser dye, nematic liquid crystal, chiral dopant, and PVA was used to prepare the dye-doped polymer-dispersed liquid crystal film by means of microcapsules. Scanning electron microscopy analysis showed that most liquid crystal droplets in the polymer matrix ranged from 30 μm to 40 μm, the size of the liquid crystal droplets was small. Under frequency doubled 532 nm Nd:YAG laser-pumped optical excitation, a plurality of discrete and sharp random laser radiation peaks could be measured in the range of 575-590 nm. The line-width of the lasing peak was 0.2 nm and the threshold of the random lasing was 9 mJ. Under heating, the emission peaks of random lasing disappeared. By detecting the emission light spot energy distribution, the mechanism of radiation was found to be random lasing. The random lasing radiation mechanism was then analyzed and discussed. Experimental results indicated that the size of the liquid crystal droplets is the decisive factor that influences the lasing mechanism. The surface anchor role can be ignored when the size of the liquid crystal droplets in the polymer matrix is small, which is beneficial to form multiple scattering. The transmission path of photons is similar to that in a ring cavity, providing feedback to obtain random lasing output. Project supported by the National Natural Science Foundation of China (Grant No. 61378042), the Colleges and Universities in Liaoning Province Outstanding Young Scholars Growth Plans, China (Grant No. LJQ2015093), and Shenyang Ligong University Laser and Optical Information of Liaoning Province Key Laboratory Open Funds, China.

Loading an Optical Trap with Diamond Nanocrystals Containing Nitrogen-Vacancy Centers from a Surface

NASA Astrophysics Data System (ADS)

Hsu, Jen-Feng; Ji, Peng; Dutt, M. V. Gurudev; D'Urso, Brian R.

2015-03-01

We present a simple and effective method of loading particles into an optical trap. Our primary application of this method is loading photoluminescent material, such as diamond nanocrystals containing nitrogen-vacancy (NV) centers, for coupling the mechanical motion of the trapped crystal with the spin of the NV centers. Highly absorptive material at the trapping laser frequency, such as tartrazine dye, is used as media to attach nanodiamonds and burn into a cloud of air-borne particles as the material is swept near the trapping laser focus on a glass slide. Particles are then trapped with the laser used for burning or transferred to a second laser trap at a different wavelength. Evidence of successful loading diamond nanocrystals into the trap presented includes high sensitivity of the photoluminecscence (PL) to the excitation laser and the PL spectra of the optically trapped particles

ICIASF '85 - International Congress on Instrumentation in Aerospace Simulation Facilities, 11th, Stanford University, CA, August 26-28, 1985, Record

NASA Technical Reports Server (NTRS)

1985-01-01

Developments related to laser Doppler velocimetry are discussed, taking into account a three-component dual beam laser-Doppler-anemometer to be operated in large wind tunnels, a new optical system for three-dimensional laser-Doppler-anemometry using an argon-ion and a dye laser, and a two-component laser Doppler velocimeter by switching fringe orientation. Other topics studied are concerned with facilities, instrumentation, control, hot wire/thin film measurements, optical diagnostic techniques, signal and data processing, facilities and adaptive wall test sections, data acquisition and processing, ballistic instrument systems, dynamic testing and material deformation measurements, optical flow measurements, test techniques, force measurement systems, and holography. Attention is given to nonlinear calibration of integral wind tunnel balances, a microcomputer system for real time digitized image compression, and two phase flow diagnostics in propulsion systems.

Stimulated emission and optical properties of pyranyliden fragment containing compounds in PVK matrix

NASA Astrophysics Data System (ADS)

Vembris, Aivars; Zarins, Elmars; Kokars, Valdis

2017-10-01

Organic solid state lasers are thoughtfully investigated due to their potential applications in communication, sensors, biomedicine, etc. Low amplified spontaneous emission (ASE) excitation threshold value is essential for further use of the material in devices. Intramolecular interaction limits high molecule density load in the matrix. It is the case of the well-known red light emitting laser dye - 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM). The lowest ASE threshold value of the mentioned laser dye could be obtained within the concentration range between 2 and 4 wt%. At higher concentration threshold energy drastically increases. In this work optical and ASE properties of three original DCM derivatives in poly(N-vinylcarbazole) (PVK) at various concentrations will be discussed. One of the derivatives is modified DCM dye in which the methyl substituents in the electron donor part have been replaced with bulky trityloxyethyl groups (DWK-1). These sterically significant functional groups do not influence electron transitions in the dye but prevent aggregation of the molecules. The chemical structure of the second investigated compound is similar to DWK-1 where the methyl group is replaced with the tert-butyl substituent (DWK-1TB). The third derivative (DWK-2) consists of two N,N-di(trityloxyethyl)amino electron donor groups. All results were compared with DCM:PVK system. Photoluminescence quantum yield (PLQY) is up to ten times larger for DWK-1TB with respect to DCM systems. Bulky trityloxyethyl groups prevent aggregation of the molecules thus decreasing interaction between dyes and amount of non-radiative decays. The red shift of the photoluminescence and amplified spontaneous emission at higher concentrations were observed due to the solid state solvation effect. The increase of the investigated dye density in the matrix with a smaller reduction in PLQY resulted in low ASE threshold energy. The lowest threshold value was obtained around 21 μJ/cm2 (2.1 kW/cm2) in DWK-1TB:PVK films.

Mobile lidar system for environmental monitoring

NASA Astrophysics Data System (ADS)

Zhao, Guangyu; Lian, Ming; Li, Yiyun; Duan, Zheng; Zhu, Shiming; Svanberg, Sune

2018-04-01

A versatile mobile remote sensing system for multidisciplinary environmental monitoring tasks on the Chinese scene is described. The system includes a 20 Hz Nd:YAG laser-pumped dye laser, optical transmitting/receiving systems with a 30 cm and a 40 cm Newtonian telescope, and electronics, all integrated in a laboratory, installed on a Jiefang truck. Results from field experiments on atomic mercury DIAL mapping and remote laser-induced fluorescence and break-down spectroscopy are given.

IV INTERNATIONAL CONFERENCE ON ATOM AND MOLECULAR PULSED LASERS (AMPL'99): Spectral properties of optical anisotropy induced by laser radiation in dye solutions

NASA Astrophysics Data System (ADS)

Pikulik, L. G.; Chernyavskii, V. A.; Grib, A. F.

2000-06-01

Spectral studies of induced quasi-crystal properties (which can be quantitatively characterised by the difference in the refractive indices of ordinary and extraordinary waves, Δn=no—ne) in Rhodamine 6G and Rhodamine 4C solutions in glycerine excited in the visible and UV ranges of the absorption spectrum are presented. It is demonstrated that the observed spectral dependences of Δn of these dye solutions excited in the visible (long-wavelength) and UV (short-wavelength) ranges of the absorption spectrum can be interpreted in terms of an oscillator model of a molecule. The proposed method for the analysis of induced optical anisotropy in solutions of organic compounds allows the relative orientation of oscillators in a molecule and, thus, the relative orientation of electronic transitions in a molecule to be determined in a reliable way.

Bonding of human meniscal and articular cartilage with photoactive 1,8-naphthalimide dyes

NASA Astrophysics Data System (ADS)

Judy, Millard M.; Nosir, Hany R.; Jackson, Robert W.; Matthews, James Lester; Lewis, David E.; Utecht, Ronald E.; Yuan, Dongwu

1996-05-01

This study focused on meniscal cartilage repair by using the laser-activated photoactive 1,8- naphthalimide dye N,N'-bis-{6-[2-(2-(2- aminoethoxy)ethoxy)ethoxyethyl]amino-1H-benz (de)isoquinolin-1,3(2H)-dion-2- yl}-1,11-diamino-3,6,9-trioxaundecane. Harvested cadaveric human menisci were debrided and carved into strips 1 mm thick, 10 mm long, and 3 mm wide. Each strip was divided into two flaps, the surface painted with photoactive dye, the painted surfaces overlapped, the sample wrapped in Saran film, and the composite sandwiched between two glass slides at a pressure of approximately 3 kg/cm2. The sample then was transilluminated by argon ion laser light of 457.9-nm wavelength at a power density of 200 mW/cm2 with exposure times up to 5 h (3902 J/cm2 energy density). Essentially, the same procedures were performed for human femoral articular cartilage samples. Control experiments were conducted with laser irradiation alone and with dye alone. All the specimens were stored in isotonic saline solution for 2 h after irradiation to ensure hydration. The bond shear-strength was then tested and samples prepared for optical and electron transmission microscopy. Shear strength values of up to 1.8 kg/cm2 for meniscal tissues and 1.2 kg/cm2 for articular cartilaginous tissues were obtained for exposures of 3902 J/cm2. Shear strength values of approximately 0.9 kg/cm2 and 0.4 kg/cm2, respectively, for meniscus and cartilage were obtained with 459 J/cm2 exposure. Dye- and light-only controls exhibited 0 kg/cm2 shear strength values. Microscopy revealed close contact at the bonded surface in the laser-activated, dye-treated-specimens. This study shows that the laser-activated photoactive dyes have the capability of athermally bonding the meniscal and articular cartilage surfaces.

Effect of wavelength and dye selection on biosensor response

NASA Astrophysics Data System (ADS)

Ligler, Frances S.; Breslin, Kristen A.; Cao, Lynn K.; Anderson, George P.

1995-05-01

The availability of low cost laser diodes and new fluorescent dyes has made portable biosensors a reality. Previously, we have examined the variation in the fluorescent signal generated in an antigen-antibody reaction when the antigen is labeled with dyes exciting at different wavelengths. In this study, we looked at the effect of changing dyes and wavelengths on a sandwich immunoassay for the F1 antigen from Yersinia pestis, the etiologic agent of plaque. The F1 immunoassay has previously been demonstrated to work in serum, plasma, and even whole blood, when performed using a fiber optic biosensor. In this study, we demonstrated that changing to cyanine dyes enhanced the sensitivity of the detection without altering the immunochemistry of the assay.

Luminescence enhancement in nanocomposite consisting of polyvinyl alcohol incorporated gold nanoparticles and Nile blue 690 perchlorate.

PubMed

Chubinidze, Ketevan; Partsvania, Besarion; Sulaberidze, Tamaz; Khuskivadze, Aleksandre; Davitashvili, Elene; Koshoridze, Nana

2014-11-01

We have experimentally demonstrated that the emission of visible light from the polymer matrix doped with luminescent dye and gold nanoparticles (GNPs) can be enhanced with the use of surface plasmon coupling. GNPs can enhance the luminescence intensity of nearby luminescent dye because of the interactions between the dipole moments of the dye and the surface plasmon field of the GNPs. The electric charge on the GNPs and the distance between GNPs and luminescent dye molecules have a significant effect on the luminescence intensity, and this enhancement depends strongly upon the excitation wavelength of the pumping laser source. In particular, by matching the plasmon frequency of GNPs to the frequency of the laser light source we have observed a strong luminescence enhancement of the nanocomposite consisting of GNPs coupled with luminescent dye Nile blue 690 perchlorate. This ability of controlling luminescence can be beneficially used in developing contrast agents for highly sensitive and specific optical sensing and imaging. This opens new possibilities for plasmonic applications in the solar energy field.

Imaging monitoring techniques applications in the transient gratings detection

NASA Astrophysics Data System (ADS)

Zhao, Qing-ming

2009-07-01

Experimental studies of Degenerate four-wave mixing (DFWM) in iodine vapor at atmospheric pressure and 0℃ and 25℃ are reported. The Laser-induced grating (LIG) studies are carried out by generating the thermal grating using a pulsed, narrow bandwidth, dye laser .A new image processing system for detecting forward DFWM spectroscopy on iodine vapor is reported. This system is composed of CCD camera, imaging processing card and the related software. With the help of the detecting system, phase matching can be easily achieved in the optical arrangement by crossing the two pumps and the probe as diagonals linking opposite corners of a rectangular box ,and providing a way to position the PhotoMultiplier Tube (PMT) . Also it is practical to know the effect of the pointing stability on the optical path by monitoring facula changing with the laser beam pointing and disturbs of the environment. Finally the effects of Photostability of dye laser on the ration of signal to noise in DFWM using forward geometries have been investigated in iodine vapor. This system makes it feasible that the potential application of FG-DFWM is used as a diagnostic tool in combustion research and environment monitoring.

Multipurpose Fiber Injected-micro-spherical LIDAR System

NASA Technical Reports Server (NTRS)

Abdelayem, Hossin; Jamison, Tracee

2005-01-01

A technological revolution is occurring in the field of fiber lasers. Over the past two years, the level of power has increased from approx. 100 watts to nearly 1 kilowatt. We are developing a novel fiber laser system, which is a satellite-based LIDAR transmitter of multi-lines. The system is made of a hollow fiber filled with micro-spheres doped with lasing materials. Each sphere has its inherent optical cavity, which makes the system a cavity free and in the same time, emits multi-laser lines for simultaneous multi-task operations. The system is also rugged, compact, lightweight, and durable. Our earlier studies on micro-spheres doped with different laser dyes demonstrated the emission of extremely fine laser lines of less than 3 A line-width, which are of interest for spectroscopic applications, sensing, imaging, and optical communications. Individual dye-doped micro-spheres demonstrated a lasing resonance peaks phenomenon in their fluorescence spectra of linear and nonlinear features that do not exist in the bulk dye solutions. Each individual micro-sphere acts as a laser system with inherent cavity, where the fluorescence line suffers multiple internal reflections within the micro-sphere and gains enough energy to become a laser line. Such resonance peaks are dependent on the sphere's morphology, size, shape, and its refractive index. These resonance peaks are named structural resonance, whispering modes or whispering gallery modes, creeping waves, circumferential waves, surfaces modes, and virtual modes. All of these names refer to the same phenomenon of morphology dependent resonance (MDR), which has already been described and predicted precisely by electromagnetic theory and Lorentz-Mie theory since 1908. The resonance peaks become more obvious when the particle size approaches and exceeds the wavelength of the laser used and the relative index of the particle is greater than that of the surrounding medium. Additional information is included in the original extended abstract.

Optofluidic lasers with a single molecular layer of gain

PubMed Central

Chen, Qiushu; Ritt, Michael; Sivaramakrishnan, Sivaraj; Sun, Yuze; Fan, Xudong

2014-01-01

We achieve optofluidic lasers with a single molecular layer of gain, in which green fluorescent protein, dye-labeled bovine serum albumin, and dye-labeled DNA are respectively used as the gain medium and attached to the surface of a ring resonator via surface immobilization biochemical methods. It is estimated that the surface density of the gain molecules is on the order of 1012/cm2, sufficient for lasing under pulsed optical excitation. It is further shown that the optofluidic laser can be tuned by energy transfer mechanisms through biomolecular interactions. This work not only opens a door to novel photonic devices that can be controlled at the level of a single molecular layer, but also provides a promising sensing platform to analyze biochemical processes at the solid-liquid interface. PMID:25312306

All optical controlled photonic integrated circuits using azo dye functionized sol-gel material

NASA Astrophysics Data System (ADS)

Ke, Xianjun

The main focus of this dissertation is development and characterization of all-optical controllable azo dye functionized sol gel material, demonstrating a PIC fabrication technique on glass substrate using such material, and exploration and feasibility demonstration of three PIC functional devices namely optical variable attenuator, optical switches, and optical tunable filters using the material. The realization of all the devices in this dissertation are based on one material: dye functionalized sol-gel material. A photochromic sol-gel material functionalized with azo dye was synthesized and characterized. It possesses a photochromic characteristic under the control of green laser beam illumination. The material characteristics suggest the possibility of a new promising material platform candidate for the fabrication of alloptical controlled photonic integrated circuits. As the first potential application of the dye functionalized sol-gel material, an alloptical variable attenuator was designed and demonstrated. The optical variable attenuation is achieved in Mach-Zehnder interferometric configuration through all-optical modulation of sol-gel waveguide phase shifters. A 2 x 2 optical switch based on multimode interference (MMI) waveguide structure is proposed in the dissertation. The schematic configuration of the optical switch consists of a cascade of two identical MMIs with two all-optical controlled phase shifters realized by using the photochromic sol-gel material. The cross or bar switch state of the optical switch is determined by the phase difference between the two sol-gel waveguide phase shifters. An all-optical tunable filter is designed and its feasibility demonstrated by using the sol-gel photochromic material. Except for the phase change demonstrated on sol-gel waveguide phase shifters, dynamic gratings were observed on sol-gel film when exposed to two interference beams. This reveals the possibility of realizing Bragg grating-based tunable filters. The schematic configuration of proposed tunable filters consists of a single straight waveguide embedded with a sol-gel waveguide. The wavelength tuning of the tunable filters is accomplished by varying the grating period.

Laser-Induced Population Inversion in Rhodamine 6G for Lysozyme Oligomer Detection.

PubMed

Hanczyc, Piotr; Sznitko, Lech

2017-06-06

Fluorescence spectroscopy is a common method for detecting amyloid fibrils in which organic fluorophores are used as markers that exhibit an increase in quantum yield upon binding. However, most of the dyes exhibit enhanced emission only when bound to mature fibrils, and significantly weaker signals are obtained in the presence of amyloid oligomers. In the concept of population inversion, a laser is used as an excitation source to keep the major fraction of molecules in the excited state to create the pathways for the occurrence of stimulated emission. In the case of the proteins, the conformational changes lead to the self-ordering and thus different light scattering conditions that can influence the optical signatures of the generated light. Using this methodology, we show it is possible to optically detect amyloid oligomers using commonly available staining dyes in which population inversion can be induced. The results indicate that rhodamine 6G molecules are complexed with oligomers, and using a laser-assisted methodology, weakly emissive states can be detected. Significant spectral red-shifting of rhodamine 6G dispersed with amyloid oligomers and a notable difference determined by comparison of spectra of the fibrils suggest the existence of specific dye aggregates around the oligomer binding sites. This approach can provide new insights into intermediate oligomer states that are believed to be responsible for toxic seeding in neurodegeneration diseases.

Inversion of the resonance line of Sr/+/ produced by optically pumping Sr atoms

NASA Technical Reports Server (NTRS)

Green, W. R.; Falcone, R. W.

1978-01-01

A description is presented of an experiment which demonstrates the selective production of excited-state ions by an optical absorption from neutrals. An inversion on the resonance line of Sr(+) was produced by laser excitation of a two-electron transition, followed by ionization of one of the excited electrons by the same laser. A pulsed, mode-locked laser operating at 2680 A was used to excite atoms from the Sr ground level. The same laser then ionized the excited atoms. The 2680-A pump beam was generated by frequency doubling the output of a synchronously pumped mode-locked dye laser in a KDP crystal. It is pointed out that the reported results are significant for the construction of vacuum-ultraviolet and X-ray lasers. Many of the proposed methods for making such lasers depend on the selective production of excited-state ions.

Fluidic optics

NASA Astrophysics Data System (ADS)

Whitesides, George M.; Tang, Sindy K. Y.

2006-09-01

Fluidic optics is a new class of optical system with real-time tunability and reconfigurability enabled by the introduction of fluidic components into the optical path. We describe the design, fabrication, operation of a number of fluidic optical systems, and focus on three devices, liquid-core/liquid-cladding (L2) waveguides, microfluidic dye lasers, and diffraction gratings based on flowing, crystalline lattices of bubbles, to demonstrate the integration of microfluidics and optics. We fabricate these devices in poly(dimethylsiloxane) (PDMS) with soft-lithographic techniques. They are simple to construct, and readily integrable with microanalytical or lab-on-a-chip systems.

High-speed, random-access fluorescence microscopy: I. High-resolution optical recording with voltage-sensitive dyes and ion indicators.

PubMed

Bullen, A; Patel, S S; Saggau, P

1997-07-01

The design and implementation of a high-speed, random-access, laser-scanning fluorescence microscope configured to record fast physiological signals from small neuronal structures with high spatiotemporal resolution is presented. The laser-scanning capability of this nonimaging microscope is provided by two orthogonal acousto-optic deflectors under computer control. Each scanning point can be randomly accessed and has a positioning time of 3-5 microseconds. Sampling time is also computer-controlled and can be varied to maximize the signal-to-noise ratio. Acquisition rates up to 200k samples/s at 16-bit digitizing resolution are possible. The spatial resolution of this instrument is determined by the minimal spot size at the level of the preparation (i.e., 2-7 microns). Scanning points are selected interactively from a reference image collected with differential interference contrast optics and a video camera. Frame rates up to 5 kHz are easily attainable. Intrinsic variations in laser light intensity and scanning spot brightness are overcome by an on-line signal-processing scheme. Representative records obtained with this instrument by using voltage-sensitive dyes and calcium indicators demonstrate the ability to make fast, high-fidelity measurements of membrane potential and intracellular calcium at high spatial resolution (2 microns) without any temporal averaging.

High-speed, random-access fluorescence microscopy: I. High-resolution optical recording with voltage-sensitive dyes and ion indicators.

PubMed Central

Bullen, A; Patel, S S; Saggau, P

1997-01-01

The design and implementation of a high-speed, random-access, laser-scanning fluorescence microscope configured to record fast physiological signals from small neuronal structures with high spatiotemporal resolution is presented. The laser-scanning capability of this nonimaging microscope is provided by two orthogonal acousto-optic deflectors under computer control. Each scanning point can be randomly accessed and has a positioning time of 3-5 microseconds. Sampling time is also computer-controlled and can be varied to maximize the signal-to-noise ratio. Acquisition rates up to 200k samples/s at 16-bit digitizing resolution are possible. The spatial resolution of this instrument is determined by the minimal spot size at the level of the preparation (i.e., 2-7 microns). Scanning points are selected interactively from a reference image collected with differential interference contrast optics and a video camera. Frame rates up to 5 kHz are easily attainable. Intrinsic variations in laser light intensity and scanning spot brightness are overcome by an on-line signal-processing scheme. Representative records obtained with this instrument by using voltage-sensitive dyes and calcium indicators demonstrate the ability to make fast, high-fidelity measurements of membrane potential and intracellular calcium at high spatial resolution (2 microns) without any temporal averaging. Images FIGURE 6 PMID:9199810

LASER BIOLOGY: Visualisation of the distributions of melanin and indocyanine green in biological tissues

NASA Astrophysics Data System (ADS)

Genina, E. A.; Fedosov, I. V.; Bashkatov, A. N.; Zimnyakov, D. A.; Altshuler, G. B.; Tuchin, V. V.

2008-03-01

A double-wavelength laser scanning microphotometer with the high spectral and spatial resolutions is developed for studying the distribution of endogenic and exogenic dyes in biological tissues. Samples of hair and skin biopsy with hair follicles stained with indocyanine green are studied. The spatial distribution of indocyanine green and melanin in the biological tissue is determined from the measured optical transmittance.

Visualisation of the distributions of melanin and indocyanine green in biological tissues

DOE Office of Scientific and Technical Information (OSTI.GOV)

Genina, E A; Fedosov, I V; Bashkatov, A N

2008-03-31

A double-wavelength laser scanning microphotometer with the high spectral and spatial resolutions is developed for studying the distribution of endogenic and exogenic dyes in biological tissues. Samples of hair and skin biopsy with hair follicles stained with indocyanine green are studied. The spatial distribution of indocyanine green and melanin in the biological tissue is determined from the measured optical transmittance. (laser biology)

Demonstration of versatile whispering-gallery micro-lasers for remote refractive index sensing.

PubMed

Wan, Lei; Chandrahalim, Hengky; Zhou, Jian; Li, Zhaohui; Chen, Cong; Cho, Sangha; Zhang, Hui; Mei, Ting; Tian, Huiping; Oki, Yuji; Nishimura, Naoya; Fan, Xudong; Guo, L Jay

2018-03-05

We developed chip-scale remote refractive index sensors based on Rhodamine 6G (R6G)-doped polymer micro-ring lasers. The chemical, temperature, and mechanical sturdiness of the fused-silica host guaranteed a flexible deployment of dye-doped polymers for refractive index sensing. The introduction of the dye as gain medium demonstrated the feasibility of remote sensing based on the free-space optics measurement setup. Compared to the R6G-doped TZ-001, the lasing behavior of R6G-doped SU-8 polymer micro-ring laser under an aqueous environment had a narrower spectrum linewidth, producing the minimum detectable refractive index change of 4 × 10 -4 RIU. The maximum bulk refractive index sensitivity (BRIS) of 75 nm/RIU was obtained for SU-8 laser-based refractive index sensors. The economical, rapid, and simple realization of polymeric micro-scale whispering-gallery-mode (WGM) laser-based refractive index sensors will further expand pathways of static and dynamic remote environmental, chemical, biological, and bio-chemical sensing.

Development of a see-through hollow cathode discharge lamp for (Li/Ne) optogalvanic studies

NASA Astrophysics Data System (ADS)

Saini, V. K.; Kumar, P.; Sarangpani, K. K.; Dixit, S. K.; Nakhe, S. V.

2017-09-01

Development of a demountable and see-through hollow cathode (HC) discharge lamp suitable for optogalvanic (OG) spectroscopy is described. The design of the HC lamp is simple, compact, and inexpensive. Lithium, investigated rarely by the OG method, is selected for cathode material as its isotopes are important for nuclear industry. The HC lamp is characterized electrically and optically for discharge oscillations free OG effect. Strong OG signals of lithium as well as neon (as buffer gas) are produced precisely upon copper vapor laser pumped tunable dye laser irradiation. The HC lamp is capable of generating a clean OG resonance spectrum in the available dye laser wavelength scanning range (627.5-676 nm) obtained with 4-(Dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran dye. About 28 resonant OG lines are explicitly observed. Majority of them have been identified using j-l coupling scheme and assigned to the well-known neon transitions. One line that corresponds to wavelength near about 670.80 nm is assigned to lithium and resolved for its fine (2S1/2 → 2P1/2, 3/2) transitions. These OG transitions allow 0.33 cm-1 accuracy and can be used to supplement the OG transition data available from other sources to calibrate the wavelength of a scanning dye laser with precision at atomic levels.

Development of a see-through hollow cathode discharge lamp for (Li/Ne) optogalvanic studies.

PubMed

Saini, V K; Kumar, P; Sarangpani, K K; Dixit, S K; Nakhe, S V

2017-09-01

Development of a demountable and see-through hollow cathode (HC) discharge lamp suitable for optogalvanic (OG) spectroscopy is described. The design of the HC lamp is simple, compact, and inexpensive. Lithium, investigated rarely by the OG method, is selected for cathode material as its isotopes are important for nuclear industry. The HC lamp is characterized electrically and optically for discharge oscillations free OG effect. Strong OG signals of lithium as well as neon (as buffer gas) are produced precisely upon copper vapor laser pumped tunable dye laser irradiation. The HC lamp is capable of generating a clean OG resonance spectrum in the available dye laser wavelength scanning range (627.5-676 nm) obtained with 4-(Dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran dye. About 28 resonant OG lines are explicitly observed. Majority of them have been identified using j-l coupling scheme and assigned to the well-known neon transitions. One line that corresponds to wavelength near about 670.80 nm is assigned to lithium and resolved for its fine ( 2 S 1/2 → 2 P 1/2, 3/2 ) transitions. These OG transitions allow 0.33 cm -1 accuracy and can be used to supplement the OG transition data available from other sources to calibrate the wavelength of a scanning dye laser with precision at atomic levels.

The family of micro sensors for remote control the pollution in liquids and gases

NASA Astrophysics Data System (ADS)

Tulaikova, Tamara; Kocharyun, Gevorg; Rogerson, Graham; Burmistrova, Ludmyla; Sychugov, Vladimir; Dorojkin, Peter

2005-10-01

There are the results for the 3 groups of fiber-optical sensors. First is the fiber-optical sensor with changed sensitive heads on the base on porous polymer with clamped activated dye. Vibration method for fiber-optical sensors provides more convenient output measurements of resonant frequency changes, in comparison with the first device. The self-focusing of the living sells into optical wave-guides in laser road in water will be considered as a new touch method for environment remote sensing.

Optical limiting properties of 3,5-dipyrenylvinyleneBODIPY dyes at 532 nm

NASA Astrophysics Data System (ADS)

Kubheka, Gugu; Sanusi, Kayode; Mack, John; Nyokong, Tebello

2018-02-01

The optical limiting (OL) properties of 3,5-dipyrenylvinyleneBODIPY dyes that contain both electron withdrawing and donating moieties have been investigated by using the z-scan technique at 532 nm in the nanosecond pulse range. The extension of the π-conjugation at the 3,5-positions with pyrenylvinylene groups results in a ca. 200 nm red shift of the main BODIPY spectral band to ca. 700 nm, so there is relatively weak absorbance at 532 nm under ambient light conditions. Reverse saturable absorbance (RSA) profiles are observed in response to incident pulsed laser light that is consistent with a two photon absorption-assisted excited state absorption (ESA) mechanism in CH2Cl2 solution and when the dyes are embedded in poly(bisphenol carbonate A) (PBC) polymer thin films. This demonstrates that 3,5-divinyleneBODIPY dyes are potentially suitable for use in OL applications, since limiting threshold fluence (Ilim) values of below 0.95 J cm-2 are observed when thin films are prepared.

Mie resonances to tailor random lasers

NASA Astrophysics Data System (ADS)

García, P. D.; Ibisate, M.; Sapienza, R.; Wiersma, D. S.; López, C.

2009-07-01

In this paper, we present an optical characterization of photonic glass-based random lasers. We show how the resonant behavior of diffuse light transport through such systems can tailor the lasing emission when a gain medium is added to the glass. A DNA-based organic dye is used as gain medium. The resonances in the transport mean-free path influence the lasing wavelength of the random laser. The laser wavelength is therefore controlled by the sphere diameter. Furthermore, the existence of Mie resonances reduces the necessary pump energy to reach the lasing threshold.

Design and Construction of Simple, Nitrogen-Laser-Pumped, Tunable Dye Lasers

ERIC Educational Resources Information Center

Hilborn, Robert C.

1978-01-01

The basic physical principles of dye lasers are discussed and used to analyze the design and operation of tunable dye lasers pumped by pulsed nitrogen lasers. Details of the design and construction of these dye lasers are presented. Some simple demonstration experiments are described. (BB)

High laser efficiency and photostability of pyrromethene dyes mediated by nonpolar solvent.

PubMed

Gupta, Monika; Kamble, Priyadarshini; Rath, M C; Naik, D B; Ray, Alok K

2015-08-10

Many pyrromethene (PM) dyes have been shown to outperform established rhodamine dyes in terms of laser efficiency in the green-yellow spectral region, but their rapid photochemical degradation in commonly used ethanol or methanol solvents continues to limit its use in high average power liquid dye lasers. A comparative study on narrowband laser efficiency and photostability of commercially available PM567 and PM597 dyes, using nonpolar n-heptane and 1,4-dioxane and polar ethanol solvents, was carried out by a constructed pulsed dye laser, pumped by the second harmonic (532 nm) radiation of a Q-switched Nd:YAG laser. Interestingly, both nonpolar solvents showed a significantly higher laser photostability (∼100 times) as well as peak efficiency (∼5%) of these PM dyes in comparison to ethanol. The different photostability of the PM dyes was rationalized by determining their triplet-state spectra and capability to generate reactive singlet oxygen (O₂1) by energy transfer to dissolved oxygen in these solvents using pulse radiolysis. Heptane is identified as a promising solvent for these PM dyes for use in high average power dye lasers, pumped by copper vapor lasers or diode-pumped solid-state green lasers.

Millimeter-wave generation and characterization of a GaAs FET by optical mixing

NASA Technical Reports Server (NTRS)

Ni, David C.; Fetterman, Harold R.; Chew, Wilbert

1990-01-01

Coherent mixing of optical radiation from a tunable continuous-wave dye laser and a stabilized He-Ne laser was used to generate millimeter-wave signals in GaAs FETs attached to printed-circuit millimeter-wave antennas. The generated signal was further down-converted to a 2-GHz IF by an antenna-coupled millimeter-wave local oscillator at 62 GHz. Detailed characterizations of power and S/N under different bias conditions have been performed. This technique is expected to allow signal generation and frequency-response evaluation of millimeter-wave devices at frequencies as high as 100 GHz.

Multispectral photoacoustic decomposition with localized regularization for detecting targeted contrast agent

NASA Astrophysics Data System (ADS)

Tavakoli, Behnoosh; Chen, Ying; Guo, Xiaoyu; Kang, Hyun Jae; Pomper, Martin; Boctor, Emad M.

2015-03-01

Targeted contrast agents can improve the sensitivity of imaging systems for cancer detection and monitoring the treatment. In order to accurately detect contrast agent concentration from photoacoustic images, we developed a decomposition algorithm to separate photoacoustic absorption spectrum into components from individual absorbers. In this study, we evaluated novel prostate-specific membrane antigen (PSMA) targeted agents for imaging prostate cancer. Three agents were synthesized through conjugating PSMA-targeting urea with optical dyes ICG, IRDye800CW and ATTO740 respectively. In our preliminary PA study, dyes were injected in a thin wall plastic tube embedded in water tank. The tube was illuminated with pulsed laser light using a tunable Q-switch ND-YAG laser. PA signal along with the B-mode ultrasound images were detected with a diagnostic ultrasound probe in orthogonal mode. PA spectrums of each dye at 0.5 to 20 μM concentrations were estimated using the maximum PA signal extracted from images which are obtained at illumination wavelengths of 700nm-850nm. Subsequently, we developed nonnegative linear least square optimization method along with localized regularization to solve the spectral unmixing. The algorithm was tested by imaging mixture of those dyes. The concentration of each dye was estimated with about 20% error on average from almost all mixtures albeit the small separation between dyes spectrums.

Dye laser amplifier including an improved window configuration for its dye beam

DOEpatents

O'Neil, R.W.; Davin, J.M.

1992-12-01

A dye laser amplifier in which a continuously replenished supply of dye is excited with a first light beam in order to amplify the intensity of a second different light beam passing therethrough is disclosed herein. This amplifier includes a cell though which a continuous stream of the dye is caused to pass, and means for directing the first beam into the cell while the second beam is directed into and through the same cell. There is also disclosed herein a specific improvement to this amplifier which resides in the use of a pair of particularly configured windows through which the second beam passes along fixed paths as the second beam enters and exits the dye cell. Each of these windows has a relatively thick main section which is substantially larger in dimensions transverse to its beam path than the cross section of the second beam itself, whereby to add structural integrity to the overall window. At the same time, the latter includes a second section which is disposed entirely within the confines of the main section and through which the second beam is intended to pass in its entirety. This second section is made substantially thinner than the main section in order to reduce optical distortion as the second beam passes therethrough. 4 figs.

Dye laser amplifier including an improved window configuration for its dye beam

DOEpatents

O'Neil, Richard W.; Davin, James M.

1992-01-01

A dye laser amplifier in which a continuously replenished supply of dye is excited with a first light beam in order to amplify the intensity of a second different light beam passing therethrough is disclosed herein. This amplifier includes a cell though which a continuous stream of the dye is caused to pass, and means for directing the first beam into the cell while the second beam is directed into and through the same cell. There is also disclosed herein a specific improvement to this amplifier which resides in the use of a pair of particularly configured windows through which the second beam passes along fixed paths as the second beam enters and exits the dye cell. Each of these windows has a relatively thick main section which is substantially larger in dimensions transverse to its beam path than the cross section of the second beam itself, whereby to add structural integrity to the overall window. At the same time, the latter includes a second section which is disposed entirely within the confines of the main section and through which the second beam is intended to pass in its entirety. This second section is made substantially thinner than the main section in order to reduce optical distortion as the second beam passes therethrough.

Solar pumped lasers: Work in progress at the University of Chicago

NASA Astrophysics Data System (ADS)

Winston, Roland

Of the variety of solar energy conversion schemes that have been explored, the conversion of solar flux to coherent laser radiation is relatively new. Solar flux at sufficiently high concentrations to overcome threshold for the really important laser materials has not been available. This technological inhibition has recently been overcome through the application of nonimaging optics through the demonstration of concentration levels of 84,000 suns at the University of Chicago in a refractive medium (sapphire) and of over 20,000 suns in air at the Solar Energy Research Institute High Flux Facility. A thermodynamic overview is presented of solar lasers including solid state lasers and dye lasers.

Experimental and computational laser tissue welding using a protein patch.

PubMed

Small, W; Heredia, N J; Maitland, D J; Eder, D C; Celliers, P M; Da Silva, L B; London, R A; Matthews, D L

1998-01-01

An in vitro study of laser tissue welding mediated with a dye-enhanced protein patch was conducted. Fresh sections of porcine aorta were used for the experiments. Arteriotomies were treated using an indocyanine green dye-enhanced collagen patch activated by an 805-nm continuous-wave fiber-delivered diode laser. Temperature histories of the surface of the weld site were obtained using a hollow glass optical fiber-based two-color infrared thermometer. The experimental effort was complemented by simulations with the LATIS (LAser-TISsue) computer code, which uses coupled Monte Carlo, thermal transport, and mass transport models. Comparison of simulated and experimental thermal data indicated that evaporative cooling clamped the surface temperature of the weld site below 100 °C. For fluences of approximately 200 J/cm2, peak surface temperatures averaged 74°C and acute burst strengths consistently exceeded 0.14×106 dyn/cm (hoop tension). The combination of experimental and simulation results showed that the inclusion of water transport and evaporative losses in the computer code has a significant impact on the thermal distributions and hydration levels throughout the tissue volume. The solid-matrix protein patch provided a means of controllable energy delivery and yielded consistently strong welds. © 1998 Society of Photo-Optical Instrumentation Engineers.

Sentinel lymph node detection by an optical method using scattered photons

PubMed Central

Tellier, Franklin; Ravelo, Rasata; Simon, Hervé; Chabrier, Renée; Steibel, Jérôme; Poulet, Patrick

2010-01-01

We present a new near infrared optical probe for the sentinel lymph node detection, based on the recording of scattered photons. A two wavelengths setup was developed to improve the detection threshold of an injected dye: the Patent Blue V dye. The method used consists in modulating each laser diode at a given frequency. A Fast Fourier Transform of the recorded signal separates both components. The signal amplitudes are used to compute relative Patent Blue V concentration. Results on the probe using phantoms model and small animal experimentation exhibit a sensitivity threshold of 3.2 µmol/L, which is thirty fold better than the eye visible threshold. PMID:21258517

Performance of laser guide star adaptive optics at Lick Observatory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Olivier, S.S.; An, J.; Avicola, K.

1995-07-19

A sodium-layer laser guide star adaptive optics system has been developed at Lawrence Livermore National Laboratory (LLNL) for use on the 3-meter Shane telescope at Lick Observatory. The system is based on a 127-actuator continuous-surface deformable mirror, a Hartmann wavefront sensor equipped with a fast-framing low-noise CCD camera, and a pulsed solid-state-pumped dye laser tuned to the atomic sodium resonance line at 589 nm. The adaptive optics system has been tested on the Shane telescope using natural reference stars yielding up to a factor of 12 increase in image peak intensity and a factor of 6.5 reduction in image fullmore » width at half maximum (FWHM). The results are consistent with theoretical expectations. The laser guide star system has been installed and operated on the Shane telescope yielding a beam with 22 W average power at 589 nm. Based on experimental data, this laser should generate an 8th magnitude guide star at this site, and the integrated laser guide star adaptive optics system should produce images with Strehl ratios of 0.4 at 2.2 {mu}m in median seeing and 0.7 at 2.2 {mu}m in good seeing.« less

Design of near-infrared dyes for nonlinear optics: toward optical limiting applications at telecommunication wavelengths

NASA Astrophysics Data System (ADS)

Bellier, Quentin; Bouit, Pierre-Antoine; Kamada, Kenji; Feneyrou, Patrick; Malmström, E.; Maury, Olivier; Andraud, Chantal

2009-09-01

The rapid development of frequency-tunable pulsed lasers up to telecommunication wavelengths (1400-1600 nm) led to the design of new materials for nonlinear absorption in this spectral range. In this context, two families of near infra-red (NIR) chromophores, namely heptamethine cyanine and aza-borondipyrromethene (aza-bodipy) dyes were studied. In both cases, they show significant two-photon absorption (TPA) cross-sections in the 1400-1600 nm spectral range and display good optical power limiting (OPL) properties. OPL curves were interpreted on the basis of TPA followed by excited state absorption (ESA) phenomena. Finally these systems have several relevant properties like nonlinear absorption properties, gram scale synthesis and high solubility. In addition, they could be functionalized on several sites which open the way to numerous practical applications in biology, solid-state optical limiting and signal processing.

Excimer-laser-induced shock wave and its dependence on atmospheric environment

NASA Astrophysics Data System (ADS)

Krueger, Ronald R.; Krasinski, Jerzy S.; Radzewicz, Czeslaw

1993-06-01

High speed shadow photography is performed on excimer laser ablated porcine corneas and rubber stoppers to capture the excimer laser induced shock waves at various time delays between 40 and 320 nanoseconds. The shock waves in air, nitrogen, and helium are recorded by tangentially illuminating the ablated surface with a tunable dye laser, the XeCl excimer laser pulse. The excimer laser ablates the specimen and excites the dye laser, which is then passed through an optical delay line before illuminating the specimen. The shadow of the shock wave produced during ablation is then cast on a screen and photographed with a CCD video camera. The system is pulsed at 30 times per second to allow a video recording of the shock wave at a fixed time delay. We conclude that high energy acoustic waves and gaseous particles are liberated during excimer laser corneal ablation, and dissipate on a submicrosecond time scale. The velocity of their dissipation is dependent on the atmospheric environment and can be increased two-fold when the ablation is performed in a helium atmosphere. Therefore, local temperature increases due to the liberation of high energy gases may be reduced by using helium during corneal photoablation.

Bench-Top Antigen Detection Technique that Utilizes Nanofiltration and Fluorescent Dyes which Emit and Absorb Light in the Near Infrared

NASA Technical Reports Server (NTRS)

Varaljay-Spence, Vanessa A.; Scardelletti, Maximilian C.

2007-01-01

This article discusses the development of a bench-top technique to detect antigens in fluids. The technique involves the use of near infrared NIR fluorescent dyes conjugated to antibodies, centrifugation, nanofilters, and spectrometry. The system used to detect the antigens utilizes a spectrometer, fiber optic cables, NIR laser, and laptop computer thus making it portable and ideally suited for desk top analysis. Using IgM as an antigen and the secondary antibody, anti-IgM conjugated to the near infrared dye, IRDye (trademark) 800, for detection, we show that nanofiltration can efficiently and specifically separate antibody-antigen complexes in solution and that the complexes can be detected by a spectrometer and software using NIR laser excitation at 778 nm and NIR dye offset emission at 804 nm. The peak power detected at 778 nm for the excitation emission and at 804 nm for the offset emission is 879 pW (-60.06 dBm) and 35.7 pW (-74.5 dBm), respectively.

Comparison of various excitation and detection schemes for dye-doped polymeric whispering gallery mode micro-lasers.

PubMed

Siegle, Tobias; Kellerer, Jonas; Bonenberger, Marielle; Krämmer, Sarah; Klusmann, Carolin; Müller, Marius; Kalt, Heinz

2018-02-05

We compare different excitation and collection configurations based on free-space optics and evanescently coupled tapered fibers for both lasing and fluorescence emission from dye-doped doped polymeric whispering gallery mode (WGM) micro-disk lasers. The focus of the comparison is on the lasing threshold and efficiency of light collection. With the aid of optical fibers, we localize the pump energy to the cavity-mode volume and reduce the necessary pump energy to achieve lasing by two orders of magnitude. When using fibers for detection, the collection efficiency is enhanced by four orders of magnitude compared to a free-space read-out perpendicular to the resonator plane. By enhancing the collection efficiency we are able to record a pronounced modulation of the dye fluorescence under continuous wave (cw) pumping conditions evoked by coupling to the WGMs. Alternatively to fibers as a collection tool, we present a read-out technique based on the detection of in-plane radiated light. We show that this method is especially beneficial in an aqueous environment as well as for size-reduced micro-lasers where radiation is strongly pronounced. Furthermore, we show that this technique allows for the assignment of transverse electric (TE) and transverse magnetic (TM) polarization to the observed fundamental modes in a water environment by performing polarization-dependent photoluminescence (PL) spectroscopy. We emphasize the importance of the polarization determination for sensing applications and verify expected differences in the bulk refractive index sensitivity for TE and TM WGMs experimentally.

Light amplification and lasing from dyes doped in DNA-complex thin films prepared by soaking method

NASA Astrophysics Data System (ADS)

Kawabe, Yutaka; Suzuki, Takemasa; Iisaka, You

2014-08-01

An alternative fabrication method for dye-doped DNA-surfactant complex films was developed and amplified spontaneous emission (ASE) and lasing under low energy optical pumping were demonstrated. In this new preparation technique, thin DNA-cethyltrimethylammonium (CTMA) complex films made by a spin coating method were stained with a hemicyanine dye by soaking them in acetone solution of the dye for one day. Molar ratio of the dye to DNA base pair for the final products was estimated to be 0.2, the value was much higher than those achieved via usual mixing method. ASE threshold value under pumping of a pulsed frequency-doubled YAG laser was about 0.3 mJ/cm2. Laser emission was also attained under the excitation with two interfering beams forming a dynamic grating of gain coefficient. Durability test indicated that 70% of their initial performance was maintained after 1 hour of continuous pumping. The technique was applied to water soluble dyes because the DNA complex was insoluble to water as well as acetone. We employed anionic Eosin Y dye, succeeding in sample formation and ASE emission. Different types of surfactants were also complexed with DNA, showing variation of emission peak wavelength. These results give a clue about the structure of the complex or interaction modes between DNA and surfactants, strongly suggesting that dye molecules are not intercalated into nor bound to DNA double strand directly, but are incorporated in the complex system via ion-exchange process or aggregating with cationic surfactants.

Electrically switchable organo–inorganic hybrid for a white-light laser source

PubMed Central

Huang, Jui-Chieh; Hsiao, Yu-Cheng; Lin, Yu-Ting; Lee, Chia-Rong; Lee, Wei

2016-01-01

We demonstrate a spectrally discrete white-light laser device based on a photonic bandgap hybrid, which is composed of a soft photonic crystal; i.e., a layer of dye-doped cholesteric liquid crystal (CLC), sandwiched between two imperfect but identical, inorganic multilayer photonic crystals. With a sole optical pump, a mono-, bi-, or tri-chromatic laser can be obtained and, through the soft photonic crystal regulated by an applied voltage, the hybrid possesses electrical tunability in laser wavelength. The three emitted spectral peaks originate from two bandedges of the CLC reflection band as well as one of the photonic defect modes in dual-mode lasing. Thanks to the optically bistable nature of CLC, such a white-light laser device can operate in quite an energy-saving fashion. This technique has potential to fulfill the present mainstream in the coherent white-light source. PMID:27324219

Peculiarities of Efficient Plasma Generation in Air and Water by Short Duration Laser Pulses

NASA Technical Reports Server (NTRS)

Adamovsky, Grigory; Floyd, Bertram M.

2017-01-01

We have conducted experiments to demonstrate an efficient generation of plasma discharges by focused nanosecond pulsed laser beams in air and provided recommendations on the design of optical systems to implement such plasma generation. We have also demonstrated generation of the secondary plasma discharge using the unused energy from the primary one. Focused nanosecond pulsed laser beams have also been utilized to generate plasma in water where we observed self-focusing and filamentation. Furthermore, we applied the laser generated plasma to the decomposition of methylene blue dye diluted in water.

Integrated Micro-Optics for Microfluidic Detection.

PubMed

Kazama, Yuto; Hibara, Akihide

2016-01-01

A method of embedding micro-optics into a microfluidic device was proposed and demonstrated. First, the usefulness of embedded right-angle prisms was demonstrated in microscope observation. Lateral-view microscopic observation of an aqueous dye flow in a 100-μm-sized microchannel was demonstrated. Then, the embedded right-angle prisms were utilized for multi-beam laser spectroscopy. Here, crossed-beam thermal lens detection of a liquid sample was applied to glucose detection.

Nonlinear optical effects in organic microstructures

NASA Astrophysics Data System (ADS)

Novikov, Vladimir B.; Mamonov, Evgeniy A.; Kopylov, Denis A.; Mitetelo, Nikolai V.; Venkatakrishnarao, D.; Narayana, YSLV; Chandrasekar, R.; Murzina, Tatiana V.

2017-05-01

Organic microstructures attract much attention due to their unique properties originating from the design of their shape and optical parameters. In this work we discuss the linear, second- and third-order nonlinear optical effects in arrays and in individual organic microstructures composed by self-assembling technique and formed randomly on top of a solid substrate. The structures under study consist of micro-spheres, -hemispheres or -frustums made of red laser dye and reveal an intense fluorescence (FL) in the visible spectral range. Importantly, that due to a high value of the refractive index and confined geometry, such micro-structures support the excitation of whispering gallery modes (WGM), which brings about strong and spectrally-selected light localization. We show that an amplification of the nonlinear optical effects is observed for these structures as compared to a homogeneous dye film of similar composition. The obtained data are in agreement with the results of the FDTD calculations performed for the structures of different dimensions. Perspectives of application of such type of organic nonlinear microresonators in optical devices are discussed.

Optical property measurements of a novel type of upconverting reporter

NASA Astrophysics Data System (ADS)

Xiao, Xudong; Herring, Michael E.; Haushalter, Jeanne; Lee, Seonkyung; Kalogerakis, Kostas S.; Faris, Gregory W.

2003-07-01

We have recently developed a new type of reporter (upconverting chelate) for biomedical diagnostics. For this reporter, the light is absorbed and emitted by a lanthanide ion, rather than an organic molecule, as is the case for a typical fluorescent dye. These materials do not photobleach and have no autofluorescent background. We focus in this paper on neodymium ions complexed with the familiar chelating agents, EDTA, DPA, DTPA and DOTA. We have performed experimental measurements with one- and two-color laser light excitation for different chelate compounds. The samples are excited using two Nd:YAG-pumped dye laser systems that provide laser light near 587 nm and 800 nm. For one-color excitation, the emitted light depends quadratically on the incident laser power, as expected. Three strongly emitting lines are observed, located near 360 nm, 387 nm, and 417 nm. We observed more efficient upconversion in EDTA although the DPA chelates show comparable ground state absorbance. We have studied the influence of temporal delay between the two laser pulses and obtained the decay lifetime of the first intermediate state in the various chelated compounds.

Optical recording of information on paper by CO2 and YAG-lasers

NASA Astrophysics Data System (ADS)

Bayev, S. G.; Bessemltsev, V. P.; Koronkevich, D. V.; Tkachuk, Y. N.

1984-09-01

Methods for outputting information from computers that have the advantages of typographic printing processes, but are distinguished by the lack of an intermediate medium are investigated. Methods for recording graphic and half-tone images are investigated that are based on layers of ink deposited on the paper in advance, as well as fixing a temperature-sensitive dye on the paper by using a focused laser beam with radiation power density of .000001 w/sq.cm. to heat the surface. IR process lasers provide good efficiency and resolution.

Highly efficient volume hologram multiplexing in thick dye-doped jelly-like gelatin.

PubMed

Katarkevich, Vasili M; Rubinov, Anatoli N; Efendiev, Terlan Sh

2014-08-01

Dye-doped jelly-like gelatin is a thick-layer self-developing photosensitive medium that allows single and multiplexed volume phase holograms to be successfully recorded using pulsed laser radiation. In this Letter, we present a method for multiplexed recording of volume holograms in a dye-doped jelly-like gelatin, which provides significant increase in their diffraction efficiency. The method is based on the recovery of the photobleached dye molecule concentration in the hologram recording zone of gel, thanks to molecule diffusion from other unexposed gel areas. As an example, an optical recording of a multiplexed hologram consisting of three superimposed Bragg gratings with mean values of the diffraction efficiency and angular selectivity of ∼75% and ∼21^', respectively, is demonstrated by using the proposed method.

Intracellular microlasers

NASA Astrophysics Data System (ADS)

Humar, Matjaž; Hyun Yun, Seok

2015-09-01

Optical microresonators, which confine light within a small cavity, are widely exploited for various applications ranging from the realization of lasers and nonlinear devices to biochemical and optomechanical sensing. Here we use microresonators and suitable optical gain materials inside biological cells to demonstrate various optical functions in vitro including lasing. We explore two distinct types of microresonator—soft and hard—that support whispering-gallery modes. Soft droplets formed by injecting oil or using natural lipid droplets support intracellular laser action. The laser spectra from oil-droplet microlasers can chart cytoplasmic internal stress (˜500 pN μm-2) and its dynamic fluctuations at a sensitivity of 20 pN μm-2 (20 Pa). In a second form, whispering-gallery modes within phagocytized polystyrene beads of different sizes enable individual tagging of thousands of cells easily and, in principle, a much larger number by multiplexing with different dyes.

Single mode pulsed dye laser oscillator

DOEpatents

Hackel, Richard P.

1992-01-01

A single mode pulsed dye laser oscillator is disclosed. The dye laser oscillator provides for improved power efficiency by reducing the physical dimensions of the overall laser cavity, which improves frequency selection capability.

Photophysical properties, photodegradation characteristics, and lasing action for coumarin dye C540A in polymeric media

NASA Astrophysics Data System (ADS)

Jones, Guilford, II; Huang, Zhennian; Pacheco, Dennis P., Jr.; Russell, Jeffrey A.

2004-07-01

Tunable solid-state dye lasers operating in the blue-green spectral region are attractive for a variety of applications. An important consideration in assessing the viability of this technology is the service life of the gain medium, which is presently limited by dye photodegradation. In this study, solid polymeric samples consisting of the coumarin dye C540A in modified PMMA were subjected to controlled photodegradation tests. The excitation laser was a flashlamp-pumped dye laser operating at 440 nm with a pulse duration of 1 μs. A complementary set of data was obtained for dye in solution phase for comparison purposes. Photophysical properties of C540A in water solution of polymethacrylic acid (PMAA) have been investigated with a view to assess the suitability of the sequestering polymer (PMAA) as an effective additive to facilitate use of a water medium for highly efficient blue-green dye lasers. Lasing action of C540A in aqueous PMAA has been realized using flashlamp-pumped laser system, yielding excellent laser efficiencies superior to that achieved in ethanolic solutions with the same dye. Laser characterization of dye in media included measurement of laser threshold, slope efficiency, pulse duration and output wavelength.

Argon dye photocoagulator for microsurgery of the interior structure of the eye

NASA Astrophysics Data System (ADS)

Wolinski, Wieslaw L.; Kazmirowski, Antoni; Kesik, Jerzy; Korobowicz, Witold; Spytkowski, Wojciech

1991-08-01

Argon-dye laser photocoagulator for the microsurgery of the interior structure of the eye is described. Some technical specifications like power stability shape of the spots and the dependence of the power on the tissue vs. wavelenght for dye laser are given. Argon-dye photocoagulator was designed and constructed including argon laser tube and dye laser in Institute of Microelectronics and Optoelectronics Technical University of Warsaw.

Feasibility of surveying pesticide coverage with airborne fluorometer

NASA Technical Reports Server (NTRS)

Stoertz, G. E.; Hemphill, W. R.

1970-01-01

Response of a Fraunhofer line discriminator (FLD) to varying distributions of granulated corncobs stained with varying concentrations of Rhodamine WT dye was tested on the ground and from an H-19 helicopter. The granules are used as a vehicle for airborne emplacement of poison to control fire ants in the eastern and southeastern United States. Test results showed that the granules are detectable by FLD but that the concentration must be too great to be practical with the present apparatus. Possible methods for enhancement of response may include: (1) increasing dye concentration; (2) incorporating with the poisoned granules a second material to carry the dye alone; (3) use of a more strongly fluorescent substance (at 5890 A); (4) modifying the time interval after dyeing, or modifying the method of dyeing; (5) modifying the FLD for greater efficiency, increased field of view or larger optics; or (6) experimenting with laser-stimulated fluorescence.

Single mode pulsed dye laser oscillator

DOEpatents

Hackel, R.P.

1992-11-24

A single mode pulsed dye laser oscillator is disclosed. The dye laser oscillator provides for improved power efficiency by reducing the physical dimensions of the overall laser cavity, which improves frequency selection capability. 6 figs.

Solvent-free fluidic organic dye lasers.

PubMed

Choi, Eun Young; Mager, Loic; Cham, Tran Thi; Dorkenoo, Kokou D; Fort, Alain; Wu, Jeong Weon; Barsella, Alberto; Ribierre, Jean-Charles

2013-05-06

We report on the demonstration of liquid organic dye lasers based on 9-(2-ethylhexyl)carbazole (EHCz), so-called liquid carbazole, doped with green- and red-emitting laser dyes. Both waveguide and Fabry-Perot type microcavity fluidic organic dye lasers were prepared by capillary action under solvent-free conditions. Cascade Förster-type energy transfer processes from liquid carbazole to laser dyes were employed to achieve color-variable amplified spontaneous emission and lasing. Overall, this study provides the first step towards the development of solvent-free fluidic organic semiconducting lasers and demonstrates a new kind of optoelectronic applications for liquid organic semiconductors.

Theory of lasing in a multiple-scattering medium

NASA Astrophysics Data System (ADS)

John, Sajeev; Pang, Gendi

1996-10-01

In several recent experiments, isotropic lasing action was observed in paints that contain rhodamine 640 dye molecules in methanol solution as gain media and titania particles as optical scatterers. These so-called paint-on laser systems are extraordinary because they are highly disordered systems. The microscopic mechanism for laser activity and the coherence properties of light emission in this multiple-light-scattering medium have not yet been elucidated. In this paper we derive the emission intensity properties of a model dye system with excited singlet and triplet electronic energy levels, which is immersed in a multiple-scattering medium with transport mean free path l*. Using physically reasonable estimates for the absorption and emission cross section for the singlet and triplet manifolds, and the singlet-triplet intersystem crossing rate, we solve the nonlinear laser rate equations for the dye molecules. This leads to a diffusion equation for the light intensity in the medium with a nonlinear intensity-dependent gain coefficient. Using this model we are able to account for nearly all of the experimentally observed properties of laser paint reported so far when l*>>λ0, the emission wavelength. This includes the dependence of the peak intensity of amplified emission on the mean free path l*, the dye concentration ρ, and the pump intensity characteristics. Our model recaptures the collapse of the emission linewidth at a specific threshold pump intensity and describes how this threshold intensity varies with l*. In addition, our model predicts a dramatic increase in the peak intensity and a further lowering of the lasing threshold for the strong scattering limit l*-->λ0. This suggests a striking enhancement of the characteristics of laser paint near the photon localization threshold in a disordered medium.

Development of a large-screen high-definition laser video projection system

NASA Astrophysics Data System (ADS)

Clynick, Tony J.

1991-08-01

A prototype laser video projector which uses electronic, optical, and mechanical means to project a television picture is described. With the primary goal of commercial viability, the price/performance ratio of the chosen means is critical. The fundamental requirement has been to achieve high brightness, high definition images of at least movie-theater size, at a cost comparable with other existing large-screen video projection technologies, while having the opportunity of developing and exploiting the unique properties of the laser projected image, such as its infinite depth-of-field. Two argon lasers are used in combination with a dye laser to achieve a range of colors which, despite not being identical to those of a CRT, prove to be subjectively acceptable. Acousto-optic modulation in combination with a rotary polygon scanner, digital video line stores, novel specialized electro-optics, and a galvanometric frame scanner form the basis of the projection technique achieving a 30 MHz video bandwidth, high- definition scan rates (1125/60 and 1250/50), high contrast ratio, and good optical efficiency. Auditorium projection of HDTV pictures wider than 20 meters are possible. Applications including 360 degree(s) projection and 3-D video provide further scope for exploitation of the HD laser video projector.

Fusion of Renewable Ring Resonator Lasers and Ultrafast Laser Inscribed Photonic Waveguides

PubMed Central

Chandrahalim, Hengky; Rand, Stephen C.; Fan, Xudong

2016-01-01

We demonstrated the monolithic integration of reusable and wavelength reconfigurable ring resonator lasers and waveguides of arbitrary shapes to out-couple and guide laser emission on the same fused-silica chip. The ring resonator hosts were patterned by a single-mask standard lithography, whereas the waveguides were inscribed in the proximity of the ring resonator by using 3-dimensional femtosecond laser inscription technology. Reusability of the integrated ring resonator – waveguide system was examined by depositing, removing, and re-depositing dye-doped SU-8 solid polymer, SU-8 liquid polymer, and liquid solvent (toluene). The wavelength reconfigurability was validated by employing Rhodamine 6G (R6G) and 3,3′-Diethyloxacarbocyanine iodide (CY3) as exemplary gain media. In all above cases, the waveguide was able to couple out and guide the laser emission. This work opens a door to reconfigurable active and passive photonic devices for on-chip coherent light sources, optical signal processing, and the investigation of new optical phenomena. PMID:27600872

Fusion of Renewable Ring Resonator Lasers and Ultrafast Laser Inscribed Photonic Waveguides.

PubMed

Chandrahalim, Hengky; Rand, Stephen C; Fan, Xudong

2016-09-07

We demonstrated the monolithic integration of reusable and wavelength reconfigurable ring resonator lasers and waveguides of arbitrary shapes to out-couple and guide laser emission on the same fused-silica chip. The ring resonator hosts were patterned by a single-mask standard lithography, whereas the waveguides were inscribed in the proximity of the ring resonator by using 3-dimensional femtosecond laser inscription technology. Reusability of the integrated ring resonator - waveguide system was examined by depositing, removing, and re-depositing dye-doped SU-8 solid polymer, SU-8 liquid polymer, and liquid solvent (toluene). The wavelength reconfigurability was validated by employing Rhodamine 6G (R6G) and 3,3'-Diethyloxacarbocyanine iodide (CY3) as exemplary gain media. In all above cases, the waveguide was able to couple out and guide the laser emission. This work opens a door to reconfigurable active and passive photonic devices for on-chip coherent light sources, optical signal processing, and the investigation of new optical phenomena.

Ultrafast Pulse Generation in an Organic Nanoparticle-Array Laser.

PubMed

Daskalakis, Konstantinos S; Väkeväinen, Aaro I; Martikainen, Jani-Petri; Hakala, Tommi K; Törmä, Päivi

2018-04-11

Nanoscale coherent light sources offer potentially ultrafast modulation speeds, which could be utilized for novel sensors and optical switches. Plasmonic periodic structures combined with organic gain materials have emerged as promising candidates for such nanolasers. Their plasmonic component provides high intensity and ultrafast nanoscale-confined electric fields, while organic gain materials offer fabrication flexibility and a low acquisition cost. Despite reports on lasing in plasmonic arrays, lasing dynamics in these structures have not been experimentally studied yet. Here we demonstrate, for the first time, an organic dye nanoparticle-array laser with more than a 100 GHz modulation bandwidth. We show that the lasing modulation speed can be tuned by the array parameters. Accelerated dynamics is observed for plasmonic lasing modes at the blue side of the dye emission.

Application of PDT for Uterine Cervical Cancer.

PubMed

Muroya, T; Kawasaki, K; Suehiro, Y; Kunugi, T; Umayahara, K; Akiya, T; Iwabuchi, H; Sakunaga, H; Sakamoto, M; Sugishita, T; Tenjin, Y

1999-01-01

We have been performing PDT using Excimer Dye Laser (EDL) or YAG-OPO laser, a type of low power laser, both of which have a considerably higher degree of tissue penetration even when compared to PDT using Argon Dye Laser (ADL).PDT is a relatively simple procedure without any bleeding and does not require anesthesia since it causes no pain. PDT is performed 48 h after intravenous injection of 1.5-2.0 mg/kg of PHE (Photofrin((R))). Precise spot irradiation is possible using a colposcope with an optical laser path. We also use a cervical probe which enables photoirradiation of the entire cervical canal.We have performed PDT on 131 cases (95 CIS, 31 dysplasia, 1 vulval dysplasia (VIN), 3 squamous cell carcinoma, microinvasion, and 1 CIS + endocervical adenocarcinoma, microinvasion). Of these cases, 127 became CR (96.9%). The first CR case was 10 years ago and no recurrence has been observed yet.PDT is extremely effective to preserve fertility. Except for sensitive reactions to sunlight, there are no noticeable side effects or difficulties related to pregnancy or delivery. We expect that in the near future PDT will be performed using diode lasers and without hospitalization due to new photosensitizers which have shorter retention times.

Membrane Vibration Analysis Above the Nyquist Limit with Fluorescence Videogrammetry

NASA Technical Reports Server (NTRS)

Dorrington, Adrian A.; Jones, Thomas W.; Danehy, Paul M.; Pappa, Richard S.

2004-01-01

A new method for generating photogrammetric targets by projecting an array of laser beams onto a membrane doped with fluorescent laser dye has recently been developed. In this paper we review this new fluorescence based technique, then proceed to show how it can be used for dynamic measurements, and how a short pulsed (10 ns) laser allows the measurement of vibration modes at frequencies several times the sampling frequency. In addition, we present experimental results showing the determination of fundamental and harmonic vibration modes of a drum style dye-doped polymer membrane tautly mounted on a 12-inch circular hoop and excited with 30 Hz and 62 Hz sinusoidal acoustic waves. The projected laser dot pattern was generated by passing the beam from a pulsed Nd:YAG laser though a diffractive optical element, and the resulting fluorescence was imaged with three digital video cameras, all of which were synchronized with a pulse and delay generator. Although the video cameras are capable of 240 Hz frame rates, the laser s output was limited to 30 Hz and below. Consequently, aliasing techniques were used to allow the measurement of vibration modes up to 186 Hz with a Nyquist limit of less than 15 Hz.

High resolution imaging of intracellular oxygen concentration by phosphorescence lifetime

PubMed Central

Kurokawa, Hiromi; Ito, Hidehiro; Inoue, Mai; Tabata, Kenji; Sato, Yoshifumi; Yamagata, Kazuya; Kizaka-Kondoh, Shinae; Kadonosono, Tetsuya; Yano, Shigenobu; Inoue, Masahiro; Kamachi, Toshiaki

2015-01-01

Optical methods using phosphorescence quenching by oxygen are suitable for sequential monitoring and non-invasive measurements for oxygen concentration (OC) imaging within cells. Phosphorescence intensity measurement is widely used with phosphorescent dyes. These dyes are ubiquitously but heterogeneously distributed inside the whole cell. The distribution of phosphorescent dye is a major disadvantage in phosphorescence intensity measurement. We established OC imaging system for a single cell using phosphorescence lifetime and a laser scanning confocal microscope. This system had improved spatial resolution and reduced the measurement time with the high repetition rate of the laser. By the combination of ubiquitously distributed phosphorescent dye with this lifetime imaging microscope, we can visualize the OC inside the whole cell and spheroid. This system uses reversible phosphorescence quenching by oxygen, so it can measure successive OC changes from normoxia to anoxia. Lower regions of OC inside the cell colocalized with mitochondria. The time-dependent OC change in an insulin-producing cell line MIN6 by the glucose stimulation was successfully visualized. Assessing the detailed distribution and dynamics of OC inside cells achieved by the presented system will be useful to understanding a physiological and pathological oxygen metabolism. PMID:26065366

DOE Office of Scientific and Technical Information (OSTI.GOV)

Koch, T.H.

Coumarin laser dyes upon excitation degrade to produce products that absorb at the lasing wavelength. This results in attenuation of dye laser output through interference of stimulated emission. The roles of singlet oxygen and excitation intensity on dye degradation were explored. Singlet oxygen is formed but its reactions with the dye do not appear to be a major cause of dye laser output deterioration. High light intensity results in dye-sensitized, solvent oligomerization to yield materials that interfere with dye-stimulated emission. 1, 4-Diazabicyclo2,2,2octane (DABCO)inhibits this oligomerization.

Dye laser amplifier including a dye cell contained within a support vessel

DOEpatents

Davin, James

1992-01-01

A large (high flow rate) dye laser amplifier in which a continous replenished supply of dye is excited by a first light beam, specifically a copper vapor laser beam, in order to amplify the intensity of a second different light beam, specifically a dye beam, passing through the dye is disclosed herein. This amplifier includes a dye cell defining a dye chamber through which a continuous stream of dye is caused to pass at a flow rate of greater than 30 gallons/minute at a static pressure greater than 150 pounds/square inch and a specifically designed support vessel for containing the dye cell.

Confocal Microscopy Imaging with an Optical Transition Edge Sensor

NASA Astrophysics Data System (ADS)

Fukuda, D.; Niwa, K.; Hattori, K.; Inoue, S.; Kobayashi, R.; Numata, T.

2018-05-01

Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photon can be measured without spectroscopic elements such as diffraction gratings. As target objects, animal cells labeled with two fluorescent dyes were irradiated with an excitation laser at an intensity below 1 μW. In our confocal system, an optical fiber-coupled TES device is used to detect photons instead of the pinhole and photomultiplier tube used in typical confocal microscopes. Photons emitted from the dyes were collected by the objective lens, and sent to the optical TES via the fiber. The TES measures the wavelength of each photon arriving in an exposure time of 70 ms, and a fluorescent photon spectrum is constructed. This measurement is repeated by scanning the target sample, and finally a two-dimensional RGB-color image is obtained. The obtained image showed that the photons emitted from the dyes of mitochondria and cytoskeletons were clearly resolved at a detection intensity level of tens of photons. TES exhibits ideal performance as a photon detector with a low dark count rate (< 1 Hz) and wavelength resolving power. In the single-mode fiber-coupled system, the confocal microscope can be operated in the super-resolution mode. These features are very promising to realize high-sensitivity and high-resolution photon spectral imaging, and would help avoid cell damage and photobleaching of fluorescence dyes.

Threshold response using modulated continuous wave illumination for multilayer 3D optical data storage

NASA Astrophysics Data System (ADS)

Saini, A.; Christenson, C. W.; Khattab, T. A.; Wang, R.; Twieg, R. J.; Singer, K. D.

2017-01-01

In order to achieve a high capacity 3D optical data storage medium, a nonlinear or threshold writing process is necessary to localize data in the axial dimension. To this end, commercial multilayer discs use thermal ablation of metal films or phase change materials to realize such a threshold process. This paper addresses a threshold writing mechanism relevant to recently reported fluorescence-based data storage in dye-doped co-extruded multilayer films. To gain understanding of the essential physics, single layer spun coat films were used so that the data is easily accessible by analytical techniques. Data were written by attenuating the fluorescence using nanosecond-range exposure times from a 488 nm continuous wave laser overlapping with the single photon absorption spectrum. The threshold writing process was studied over a range of exposure times and intensities, and with different fluorescent dyes. It was found that all of the dyes have a common temperature threshold where fluorescence begins to attenuate, and the physical nature of the thermal process was investigated.

Laser dye DCM: CW, synchronously pumped, cavity pumped and single-frequency performance

NASA Astrophysics Data System (ADS)

Marason, E. G.

1981-04-01

Laser dye DCM exhibits a tuning range of 605 to 725 nm with a lasing efficiency as high as 34% when pumped by the 488 nm line of the argon ion laser, placing it among the most efficient and broadly tunable dyes known. Performance of the dye is characterized for four laser systems: 1) continuous wave, 2) synchronously pumped (SP), 3) cavity dumped synchrompously pumped (SPCD) and 4) single-frequency ring dye laser. Pulse peak powers were as high as 520 W and 2.8 kW for SP and SPCD systems respectively.

Laser micromachining of optical devices

NASA Astrophysics Data System (ADS)

Kopitkovas, Giedrius; Lippert, Thomas; David, Christian; Sulcas, Rokas; Hobley, Jonathan; Wokaun, Alexander J.; Gobrecht, Jens

2004-10-01

The combination of a gray tone phase mask with a laser assisted wet etching process was applied to fabricate complex microstructures in UV transparent dielectric materials. This one-step method allows the generation of arrays of plano-convex and Fresnel micro-lenses using a conventional XeCl excimer laser and an absorbing liquid, which is in contact with the UV transparent material. An array of plano-convex micro-lenses was tested as beam homogenizer for a high power XeCl excimer and ps Nd:YAG laser. The roughness of the etched features varies from several μm to 10 nm, depending on the laser fluence and concentration of the dye in the organic liquid. The etching process can be divided into several etching mechanisms which vary with laser fluence.

Dye laser amplifier including a low turbulence, stagnation-free dye flow configuration

DOEpatents

Davin, J.

1992-12-01

A large (high flow rate) dye laser amplifier in which a continuous replenished supply of dye is excited by a first light beam, specifically a copper vapor laser beam, in order to amplify the intensity of a second different light beam, specifically a dye beam, passing through the dye is disclosed herein. This amplifier includes a dye cell defining a dye chamber through which a continuous stream of dye is caused to pass at a flow rate of for example 30 gallons/minute, a specifically designed support vessel for containing the dye cell and a screen device for insuring that the dye stream passes into the dye cell in a substantially turbulent free, stagnation-free manner. 9 figs.

Bibliography of Soviet Laser Developments: January - February 1988

DTIC Science & Technology

1989-02-21

Organic Dyes a. Miscellaneous 64. Alekseyeva,V.I.; Butakov,A.L.; Konin,V.N.; Lazareva,T.V.; Mirza,S.Yu.; Savvina,L.P.; Sukhanov ,V.B. 0. Study on the...laser with a longitudinal discharge. IVTAN. Preprint, no. 5-220, 1987, 1-40. (RZFZA, 88/1G508). 122. Yelayev,V.F.; Mirza,S.Yu.; Sukhanov ,V.B.; Troitskiy...Klyachkin,L.Ye.; Sukhanov ,V. L. 0. Technological possibilities for electric decoupling of photodetector mosaic elements used for optical image processing

Feedback quantum control of molecular electronic population transfer

NASA Astrophysics Data System (ADS)

Bardeen, Christopher J.; Yakovlev, Vladislav V.; Wilson, Kent R.; Carpenter, Scott D.; Weber, Peter M.; Warren, Warren S.

1997-11-01

Feedback quantum control, where the sample `teaches' a computer-controlled arbitrary lightform generator to find the optimal light field, is experimentally demonstrated for a molecular system. Femtosecond pulses tailored by a computer-controlled acousto-optic pulse shaper excite fluorescence from laser dye molecules in solution. Fluorescence and laser power are monitored, and the computer uses the experimental data and a genetic algorithm to optimize population transfer from ground to first excited state. Both efficiency (the ratio of excited state population to laser energy) and effectiveness (total excited state population) are optimized. Potential use as an `automated theory tester' is discussed.

Fundamental characteristics of degradation-recoverable solid-state DFB polymer laser.

PubMed

Yoshioka, Hiroaki; Yang, Yu; Watanabe, Hirofumi; Oki, Yuji

2012-02-13

A novel solid-state dye laser with degradation recovery was proposed and demonstrated. Polydimethylsiloxane was used as a nanoporous solid matrix to enable the internal circulation of dye molecules in the solid state. An internal circulation model for the dye molecules was also proposed and verified numerically by assuming molecular mobility and using a proposed diffusion equation. The durability of the laser was increased 20.5-fold compared with that of a conventional polymethylmethacrylate laser. This novel laser solves the low-durability problem of dye-doped polymer lasers.

Feasibility of solar-pumped dye lasers

NASA Technical Reports Server (NTRS)

Lee, Ja H.; Kim, Kyung C.; Kim, Kyong H.

1987-01-01

Dye laser gains were measured at various pump-beam irradiances on a dye cell in order to evaluate the feasibility of solar pumping. Rhodamine 6G dye was considered as a candidate for the solar-pumped laser because of its high utilization of the solar spectrum and high quantum efficiency. Measurements show that a solar concentration of 20,000 is required to reach the threshold of the dye.

Hair ignition by dye laser for port-wine stain: risk factors evaluated.

PubMed

Molin, L; Hallgren, S

1999-04-01

Flashlamp-pumped pulsed dye laser is the preferred treatment for port-wine stain. Vascular hemoglobin and epidermal melanin are competing sites for dye laser absorption and damage. The case presented illustrates the potential hazard of ignition induced by dye laser treatment on the face of a patient receiving inhalation anesthesia. A 6-year-old girl with almost black hair was treated for a port-wine stain covering most of the right half of her face. She was treated with dye laser under general anesthesia administered by mask. A laser pulse close to the upper part of the eyebrow induced a blaze and the eyebrow was instantly destroyed by the fire. Regrowth of the eyebrow was complete after a few months. Hair specimens of various colors were exposed experimentally to dye laser irradiation in room and oxygen-saturated atmospheres. Risk factors of ignition are high laser dosage, a high oxygen level, repeated pulses and dark colored hair.

Preparation of 6-hydroxyindolines and their use for preparation of novel laser dyes

DOEpatents

Field, George F.; Hammond, Peter R.

1993-01-01

A novel method for the synthesis of 6-hydroxyindolines and new fluorescent dyes produced therefrom, which dyes are ring-constrained indoline-based rhodamine class dyes. These dyes have absorption and emission spectra which make them particularly useful in certain dye laser applications.

Comparison of light absorption levels with different skin phantoms and the Monte Carlo simulation using Fourier-domain optical coherence tomography

NASA Astrophysics Data System (ADS)

Jo, Hang Chan; Kim, Jae Hun; Kim, Dae Yu

2018-02-01

Dermatologic patients have various skin characteristics such as skin tone and pigmentation color. However most studies on laser ablation and treatment only considered laser operating conditions like wavelength, output power and pulse duration. The laser ablation arises from photothermal effect by photon energy absorption. Chromophores like melanin exist as the absorber in the skin. In this study, we painted color to mimic chromophores on in-vivo and in-vitro skin models to demonstrate influence on the laser ablation by skin color. Water-based pens were used to paint color. Cross sectional images of the laser ablation were acquired by Fourier-domain optical coherence tomography (Fd-OCT). Light source to make ablation was a Q-switch diode-pumped Nd:YVO4 nanosecond laser (532nm central wavelength). Irradiated light energy dose of the laser could not make ablation craters in the control group. However experimental groups showed craters with same irradiation light energy dose. These results show painting on skin increased tissue damage by absorption in painted color without dyeing cells or tissues.

Absorption and emission spectra of Li atoms trapped in rare gas matrices

NASA Astrophysics Data System (ADS)

Wright, J. J.; Balling, L. C.

1980-10-01

Pulsed-dye-laser excitation has been used to investigate the optical absorption and emission spectra of Li atoms trapped in Ar, Kr, and Xe matrices at 10 °K. Attempts to stabilize Li atoms in a Ne matrix at 2 °K were unsuccessful. Results for all three rare gases were qualitatively the same. White light absorption scans showed a single absorption with three peaks centered near the free-atom 2s→2p transition wavelength. The intensity of fluorescence produced by dye-laser excitation within this absorption band was measured as a function of emission wavelength. Excitation of the longest- and shortest-wavelength absorption peaks produced identical emission profiles, but no distinct fluorescence signal was detected when the laser was tuned to the central absorption peaks, indicating that the apparent absorption triplet is actually the superposition of a singlet and a doublet absorption originating from two different trapping sites. No additional absorption bands were detected.

Study of resonance light scattering for remote optical probing

NASA Technical Reports Server (NTRS)

Penney, C. M.; Morey, W. W.; St. Peters, R. L.; Silverstein, S. D.; Lapp, M.; White, D. R.

1973-01-01

Enhanced scattering and fluorescence processes in the visible and UV were investigated which will enable improved remote measurements of gas properties. The theoretical relationship between scattering and fluorescence from an isolated molecule in the approach to resonance is examined through analysis of the time dependence of re-emitted light following excitation of pulsed incident light. Quantitative estimates are developed for the relative and absolute intensities of fluorescence and resonance scattering. New results are obtained for depolarization of scattering excited by light at wavelengths within a dissociative continuum. The experimental work was performed in two separate facilities. One of these utilizes argon and krypton lasers, single moded by a tilted etalon, and a 3/4 meter double monochromator. This facility was used to determine properties of the re-emission from NO2, I2 and O3 excited by visible light. The second facility involves a narrow-line dye laser, and a 3/4 meter single monochromator. The dye laser produces pulsed light with 5 nsec pulse duration and 0.005 nm spectral width.

Optimization of Nanocomposite Solar Cell/Liquid Crystal Matrix to Diminish High Intensity Laser Light Relevant to Aviation Safety Applications

NASA Astrophysics Data System (ADS)

Hofmann, James A.

An increasing threat to the aviation industry is laser light illumination on airplanes during critical phases of flight. If a laser hits the cockpit, it not only distracts the pilots, but it can cause flash blindness or permanently damage the vision of the pilots. This research attempts to mitigate these lasers illuminations through the application of both liquid crystal (LC's) technologies and dye sensitized solar cell (DSSC) technologies. The LC of choice is N-(4-Methoxybenzylidene)-4-butylaniline, or MBBA, because it has special optical properties including the ability to undergo phase transitions when exposed to an electric field. By applying an external electric field, MBBA switches from its transparent nematic phase, to its non-transparent crystalline phase, blocking the laser light. This research optimized the application of MBBA by reducing the triggering voltage and relaxation time of the LC using spacer thicknesses and scratching techniques. The liquid to solid phase transition was reduced to a 3V differential, and the time required for the crystals to relax into its transparent liquid phase was reduced to less than ten seconds. The phase transition was studied using an external electric field generated by DSSCs constructed from a titanium dioxide (TiO2) nanocomposite layer coated with dye. To maximize the voltage output by the DSSCs, layer thickness and dye sensitizer were studied to investigate their impact on the performance of the DSSC when illuminated by solar lamps and green light (532nm). Three different layer thicknesses and five different dyes were tested: Eosin Y, Eriochrome Black, Congo Red, Fast Green, and Alizarine Yellow. The experimental results showed a thin layer of nanocomposite sensitized with Eosin Y dye produced the most efficient DSSCs for the scope of this research. Experimental testing showed the DSSCs can generate 381 +/- 10mV under solar lamp exposure, 356 +/- 10mV under laser light exposure, and a voltage increase of 60 +/- 16mV when exposed to both light sources. Additionally, the performance of the DSSCs were correlated to molecular modeling predictions using Spartan software. The stability of TiO2-dye interactions indicated that dye adsorption to the surface of the nanocomposite directly impacted the performance of the DSSCs. Implementation of a LC and DSSC system forces the LCs to transition between its nematic and crystalline phases depending on the wavelength of light that is illuminating the DSSC. This research explores the practicality of using LCs and DSSCs as a preliminary approach to mitigating green laser light illumination on aircraft. Experimental results have shown that DSSCs alone are not capable of forcing a phase transitions in LCs which can entirely mitigate incoming laser light. The intense laser light required to generate substantial voltage (3V) from the DSSCs penetrates the crystalline phase of the LC with minimal attenuation of 5%.

Excitation/Detection Strategies for OH Planar Laser-Induced Fluorescence Measurements in the Presence of Interfering Fuel Signal and Absorption Effects

NASA Technical Reports Server (NTRS)

Heath, Christopher M.; Anderson, Robert C.; Hicks, Yolanda R.

2011-01-01

Planar laser-induced fluorescence (PLIF) excitation/detection methods have been applied to obtain spatial distributions of the hydroxyl [OH] reacting intermediary and hydrocarbon [HC] primary species in laminar and turbulent combustion reactions. In this report, broadband and narrowband excitation/filtering techniques are explored to identify an optimal experimental configuration yielding significant fluorescent signal with low absorption losses. The combustion environments analyzed include 1) a laminar non-premixed methane/air flame and 2) a turbulent, non-premixed Jet-A/air fueled flame within a lean flame tube combustor. Hydrocarbon-based fuel and OH were excited via the R1 (1), R1(10) and R2(7) transitions of the A(sup 2)Epsilon(+) X(sup 2)pi(1,0) band using a broadband Nd:YAG pumped optical parametric oscillator (OPO) and narrowband Nd:YAG/dye laser with ultraviolet frequency extension (UVX) package. Variables tested for influence on fluorescent signal and absorption characteristics were excitation line, laser energy, exciting linewidth, combustion reactants, and test flow conditions. Results are intended to guide the transition from a dye/UVX laser to an OPO system for performing advanced diagnostics of low-emission combustion concepts.

Preparation of 6-hydroxyindolines and their use for preparation of novel laser dyes

DOEpatents

Field, G.F.; Hammond, P.R.

1993-10-26

A novel method is described for the synthesis of 6-hydroxyindolines and new fluorescent dyes produced therefrom, which dyes are ring-constrained indoline-based rhodamine class dyes. These dyes have absorption and emission spectra which make them particularly useful in certain dye laser applications.

A modified commercial ultrasound scanner used for in vivo photoacoustic imaging of nude mice injected with non-targeted contrast agents

NASA Astrophysics Data System (ADS)

Jankovic, Ladislav; Shahzad, Khalid; Wang, Yao; Burcher, Michael; Scholle, Frank-Detlef; Hauff, Peter; Mofina, Sabine; Skobe, Mihaela

2008-02-01

Photoacoustic (PA) experiments were performed using a modified commercial ultrasound scanner equipped with an array transducer and a Nd:YAG pumped OPO laser. The contrast agent SIDAG (Bayer Schering Pharma AG, Germany), used to enhance the optical absorption, demonstrated an expected pharmacokinetic behavior of the dye in the tumor and in the bladder of the nude mice. A typical behavior in the tumor consisted of an initial linear increase in PA signal followed by an exponential decay. PA signal approached the pre-injection level after about one hour following the dye injection, which was consistent with the behavior for such contrast agents when used in other imaging modalities, such as fluorescence imaging. The in-vivo spectral PA data from the mouse bladder, conducted 1.5 hours after the dye injection, clearly demonstrated presence of the dye. The multi-spectral PA data was obtained at 760nm, 784nm and 850nm laser excitations. The PA intensities obtained at these three wavelengths accurately matched the dye absorption spectrum. In addition, in the kidney, a clearance organ for this contrast agent, both in-vivo and ex-vivo results demonstrated a significant increase (~ 40%) in the ratio of PA signal at 760nm (the peak of the dye absorption) relative to the signal at 850nm (<1% absorption), indicating significant amounts of the dye in this organ. Our initial results confirm the desired photoacoustic properties of the contrast agent, indicating its great potential to be used for imaging with a commercial array-based ultrasound scanner.

MRF, ELSM and STED: tools to study defects in fused silica optics

NASA Astrophysics Data System (ADS)

Catrin, R.; Taroux, D.; Cormont, P.; Maunier, C.; Neauport, J.

2013-11-01

The MegaJoule laser being constructed at the CEA near Bordeaux (France) is designed to focus more than 1 MJ of energy at 351 nm, on a millimetre scale target in the centre of an experiment chamber. The final optic assembly of this system operating at a wavelength of 351 nm is made up of large fused silica optics, working in transmission, that are used to convey and focus the laser beam. Under high fluences (i.e. more than 5 J/cm2 for 3 ns pulses), the limited lifetime of final optical assembly is a major concern for fusion scale laser facilities. Previous works have shown that surface finishing processes applied to manufacture these optical components can leave subsurface cracks (SSD), pollution or similar defects that act as initiators of the laser damage. In this work, we used epi-fluorescent light scanning microscopy (ELSM) and Stimulated Emission Depletion (STED) in confocal mode with fluorescent dye tagging to get a better knowledge of size and depth of these subsurface cracks. Magnetorheological fluid finishing technique (MRF) was also used as a tool to remove these cracks and thus assess depths measured by confocal microscopy. Subsurface cracks with a width of about 120 nm are observed up to ten micrometers below the surface.

Rapid multi-wavelength optical assessment of circulating blood volume without a priori data

NASA Astrophysics Data System (ADS)

Loginova, Ekaterina V.; Zhidkova, Tatyana V.; Proskurnin, Mikhail A.; Zharov, Vladimir P.

2016-03-01

The measurement of circulating blood volume (CBV) is crucial in various medical conditions including surgery, iatrogenic problems, rapid fluid administration, transfusion of red blood cells, or trauma with extensive blood loss including battlefield injuries and other emergencies. Currently, available commercial techniques are invasive and time-consuming for trauma situations. Recently, we have proposed high-speed multi-wavelength photoacoustic/photothermal (PA/PT) flow cytometry for in vivo CBV assessment with multiple dyes as PA contrast agents (labels). As the first step, we have characterized the capability of this technique to monitor the clearance of three dyes (indocyanine green, methylene blue, and trypan blue) in an animal model. However, there are strong demands on improvements in PA/PT flow cytometry. As additional verification of our proof-of-concept of this technique, we performed optical photometric CBV measurements in vitro. Three label dyes—methylene blue, crystal violet and, partially, brilliant green—were selected for simultaneous photometric determination of the components of their two-dye mixtures in the circulating blood in vitro without any extra data (like hemoglobin absorption) known a priori. The tests of single dyes and their mixtures in a flow system simulating a blood transfusion system showed a negligible difference between the sensitivities of the determination of these dyes under batch and flow conditions. For individual dyes, the limits of detection of 3×10-6 M‒3×10-6 M in blood were achieved, which provided their continuous determination at a level of 10-5 M for the CBV assessment without a priori data on the matrix. The CBV assessment with errors no higher than 4% were obtained, and the possibility to apply the developed procedure for optical photometric (flow cytometry) with laser sources was shown.

Absolute tracer dye concentration using airborne laser-induced water Raman backscatter

NASA Technical Reports Server (NTRS)

Hoge, F. E.; Swift, R. N.

1981-01-01

The use of simultaneous airborne-laser-induced dye fluorescence and water Raman backscatter to measure the absolute concentration of an ocean-dispersed tracer dye is discussed. Theoretical considerations of the calculation of dye concentration by the numerical comparison of airborne laser-induced fluorescence spectra with laboratory spectra for known dye concentrations using the 3400/cm OH-stretch water Raman scatter as a calibration signal are presented which show that minimum errors are obtained and no data concerning water mass transmission properties are required when the laser wavelength is chosen to yield a Raman signal near the dye emission band. Results of field experiments conducted with an airborne conical scan lidar over a site in New York Bight into which rhodamine dye had been injected in a study of oil spill dispersion are then indicated which resulted in a contour map of dye concentrations, with a minimum detectable dye concentration of approximately 2 ppb by weight.

[INVITED] On the mechanisms of single-pulse laser-induced backside wet etching

NASA Astrophysics Data System (ADS)

Tsvetkov, M. Yu.; Yusupov, V. I.; Minaev, N. V.; Akovantseva, A. A.; Timashev, P. S.; Golant, K. M.; Chichkov, B. N.; Bagratashvili, V. N.

2017-02-01

Laser-induced backside wet etching (LIBWE) of a silicate glass surface at interface with a strongly absorbing aqueous dye solution is studied. The process of crater formation and the generated optoacoustic signals under the action of single 5 ns laser pulses at the wavelength of 527 nm are investigated. The single-pulse mode is used to avoid effects of incubation and saturation of the etched depth. Significant differences in the mechanisms of crater formation in the ;soft; mode of laser action (at laser fluencies smaller than 150-170 J/cm2) and in the ;hard; mode (at higher laser fluencies) are observed. In the ;soft; single-pulse mode, LIBWE produces accurate craters with the depth of several hundred nanometers, good shape reproducibility and smooth walls. Estimates of temperature and pressure of the dye solution heated by a single laser pulse indicate that these parameters can significantly exceed the corresponding critical values for water. We consider that chemical etching of glass surface (or molten glass) by supercritical water, produced by laser heating of the aqueous dye solution, is the dominant mechanism responsible for the formation of crater in the ;soft; mode. In the ;hard; mode, the produced craters have ragged shape and poor pulse-to-pulse reproducibility. Outside the laser exposed area, cracks and splits are formed, which provide evidence for the shock induced glass fracture. By measuring the amplitude and spectrum of the generated optoacoustic signals it is possible to conclude that in the ;hard; mode of laser action, intense hydrodynamic processes induced by the formation and cavitation collapse of vapor-gas bubbles at solid-liquid interface are leading to the mechanical fracture of glass. The LIBWE material processing in the ;soft; mode, based on chemical etching in supercritical fluids (in particular, supercritical water) is very promising for structuring of optical materials.

Tunable Optical Tweezers for Wavelength-dependent Measurements

DTIC Science & Technology

2012-04-23

have been studied in an optical levitation scheme over short laser wavelength ranges20 and for dye-loaded di- electric particles.21 In the first case...M. Block, IEEE J. Sel. Top. Quantum Electron. 2, 1066 (1996). 7K. Dholakia, W. M. Lee, L. Paterson, M. P. MacDonald, I. Andreev, P. Mthunzi, C. T. A...Brown, R. F. Marchington, and A. C. Riches, IEEE J. Sel. Top. Quantum Electron. 13, 1646 (2007). 8K. Dholakia, M. P. MacDonald, P. Zemanek, and T

Theoretical studies of solar-pumped lasers

NASA Technical Reports Server (NTRS)

Harries, W. L.

1983-01-01

Possible types of lasers were surveyed for solar power conversion. The types considered were (1) liquid dye lasers, (2) vapor dye lasers, and (3) nondissociative molecular lasers. These are discussed.

Dichroic Liquid Crystal Displays

NASA Astrophysics Data System (ADS)

Bahadur, Birendra

The following sections are included: * INTRODUCTION * DICHROIC DYES * Chemical Structure * Chemical and Photochemical Stability * THEORETICAL MODELLING * DEFECTS CAUSED BY PROLONGED LIGHT IRRADIATION * CHEMICAL STRUCTURE AND PHOTOSTABILITY * OTHER PARAMETERS AFFECTING PHOTOSTABILITY * CELL PREPARATION * DICHROIC PARAMETERS AND THEIR MEASUREMENTS * Order Parameter and Dichroic Ratio Of Dyes * Absorbance, Order Parameter and Dichroic Ratio Measurements * IMPACT OF DYE STRUCTURE AND LIQUID CRYSTAL HOST ON PHYSICAL PROPERTIES OF A DICHROIC MIXTURE * Order Parameter and Dichroic Ratio * EFFECT OF LENGTH OF DICHROIC DYES ON THE ORDER PARAMETER * EFFECT OF THE BREADTH OF DYE ON THE ORDER PARAMETER * EFFECT OF THE HOST ON THE ORDER PARAMETER * TEMPERATURE VARIATION OF THE ORDER PARAMETER OF DYES IN A LIQUID CRYSTAL HOST * IMPACT OF DYE CONCENTRATION ON THE ORDER PARAMETER * Temperature Range * Viscosity * Dielectric Constant and Anisotropy * Refractive Indices and Birefringence * solubility43,153-156 * Absorption Wavelength and Auxochromic Groups * Molecular Engineering of Dichroic Dyes * OPTICAL, ELECTRO-OPTICAL AND LIFE PARAMETERS * Colour And CIE Colour space120,160-166 * CIE 1931 COLOUR SPACE * CIE 1976 CHROMATICITY DIAGRAM * CIE UNIFORM COLOUR SPACES & COLOUR DIFFERENCE FORMULAE120,160-166 * Electro-Optical Parameters120 * LUMINANCE * CONTRAST AND CONTRAST RATIO * SWITCHING SPEED * Life Parameters and Failure Modes * DICHROIC MIXTURE FORMULATION * Monochrome Mixture * Black Mixture * ACHROMATIC BLACK MIXTURE FOR HEILMEIER DISPLAYS * Effect of Illuminant on Display Colour * Colour of the Field-On State * Effect of Dye Linewidth * Optimum Centroid Wavelengths * Effect of Dye Concentration * Mixture Formulation Using More Than Three Dyes * ACHROMATIC MIXTURE FOR WHITE-TAYLOR TYPE DISPLAYS * HEILMEIER DISPLAYS * Theoretical Modelling * Threshold Characteristic * Effects of Dye Concentration on Electro-optical Parameters * Effect of Cholesteric Doping * Effect of Alignment * Effect of Thickness * Impact of Order Parameter * Impact of the Host * Impact of Polarizer * Colour Applications * Multiplexing * QUARTER WAVE PLATE DICHROIC DISPLAYS * Operational Principle and Display Configuration11-13 * Electro-Optical Performance * DYE-DOPED TN DISPLAYS * Threshold Characteristic, Contrast Ratio and Switching Speed * PHASE CHANGE EFFECT DICHROIC LCDs * Theoretical Background * Threshold Characteristic and Molecular Orientation * MOLECULAR ORIENTATION DURING FIELD-INDUCED PHASE TRANSITION WITH HOMOGENEOUS WALL ALIGNMENT * MOLECULAR ORIENTATION DURING FIELD-INDUCED PHASE TRANSITION WITH HOMEOTROPIC WALL ALIGNMENT * Contrast Ratio, Transmission, Brightness and Switching Speed3,7,10,198-214 * Memory or Reminiscent Contrast * Electro-optical Performance vs. Temperature * Multiplexing Phase Change Dichroic LCDs * DOUBLE CELL DICHROIC LCDs3,9,14-17,232-234 * Double Cell Nematic Dichroic LCD3,8,9,14,15,233 * Double Cell One Pitch Cholesteric LCD16,17 * Double Cell Phase Change Dichroic LCD214,232 * POSITIVE MODE DICHROIC LCDS3,8,9 * Positive Mode Heilmeier Cells3,8,9,43,77,78,235-238 * USING PLEOCHROIC DYES3,8,9,43,235-238 * USING NEGATIVE DICHROIC DYES3,8,9,63,77,78156 * DUAL FREQUENCY ADDRESSED DICHROIC DISPLAYS75,238 * Positive Mode Dichroic LCDs Using λ/4 Plate * Positive Mode Double Cell Dichroic LCD * Positive Mode Dichroic LCDs Using Special Electrode patterns7,8,239-241 * Positive Mode Phase Change Dichroic LCDs3,8,9,230,243-248 * Dichroic LCDs Using an Admixture of Pleochroic and Negative Dichroic Dyes78,118 * SUPERTWIST DICHROIC EFFECT (SDE) DISPLAYS21-23 * FERROELECTRIC DICHROIC LCDs24-27 * Devices Using A Single Polarizer * Devices Using No Polarizer24-27 * POLYMER DISPERSED DICHROIC LCDs28-30,252-259 * DICHROIC POLYMER LIQUID CRYSTAL DISPLAYS * Heilmeier Type Displays * Guest-Host Cell Using an Admixture Of L.C. Polymer and Low Molecular Weight Liquid Crysta As Host * Polymeric Ferroelectric Dichroic LCDs * SMECTIC A DICHROIC LCDs * Laser Addressed Dichroic SA Displays * Thermally and Electrically Addressed Dichroic SA Displays * FLUORESCENT DICHROIC LCDs * ACKNOWLEDGEMENTS * REFERENCES

A switchable digital microfluidic droplet dye-laser.

PubMed

Kuehne, Alexander J C; Gather, Malte C; Eydelnant, Irwin A; Yun, Seok-Hyun; Weitz, David A; Wheeler, Aaron R

2011-11-07

Digital microfluidic devices allow the manipulation of droplets between two parallel electrodes. These electrodes can act as mirrors generating a micro-cavity, which can be exploited for a droplet dye-laser. Three representative laser-dyes with emission wavelengths spanning the whole visible spectrum are chosen to show the applicability of this concept. Sub-microlitre droplets of laser-dye solution are moved in and out of a lasing site on-chip to down-convert the UV-excitation light into blue, green and red laser-pulses. This journal is © The Royal Society of Chemistry 2011

Femtosecond laser-induced cell-cell surgical attachment.

PubMed

Katchinskiy, Nir; Godbout, Roseline; Goez, Helly R; Elezzabi, Abdulhakem Y

2014-04-01

Laser-induced cell-cell surgical attachment using femtosecond laser pulses is reported. We have demonstrated the ability to attach single cells using sub-10 femtosecond laser pulses, with 800 nm central wavelength delivered from a Ti:Sapphire laser. To check that the cells did not go through a cell-fusion process, a fluorescent dye Calcein AM was used to verify that the fluorescent dye did not migrate from a dyed cell to a non-dyed cell. The mechanical integrity of the attached joint was assessed using an optical tweezer. Attachment of cells was performed without the induction of cell-cell fusion, with attachment efficiency of 95%, and while preserving the cells' viability. Cell-cell attachment was achieved by delivery of one to two trains of femtosecond laser pulses lasting 15 ms each. Laser-induced ionization process led to an ultrafast reversible destabilization of the phospholipid layer of the cellular membrane. The inner cell membrane remained intact during the attachment procedure, and isolation of the cells' cytoplasm from the surrounding medium was obtained. A strong physical attachment between the cells was obtained due to the bonding of the membranes' ionized phospholipid molecules and the formation of a joint cellular membrane at the connection point. The cellular attachment technique, femtosecond laser-induced cell-cell surgical attachment, can potentially provide a platform for the creation of engineered tissue and cell cultures. © 2014 Wiley Periodicals, Inc.

The selective and efficient laser ion source and trap project LIST for on-line production of exotic nuclides

NASA Astrophysics Data System (ADS)

Wendt, Klaus; Gottwald, Tina; Hanstorp, Dag; Mattolat, Christoph; Raeder, Sebastian; Rothe, Sebastian; Schwellnus, Fabio; Havener, Charles; Lassen, Jens; Liu, Yuan

2010-02-01

Laser ion sources based on resonant excitation and ionization of atoms are well-established tools for selective and efficient production of radioactive ion beams. A recent trend is the complementary installation of reliable state-of-the-art all solid-state Ti:Sapphire laser systems. To date, 35 elements of the Periodic Table are available at laser ion sources by using these novel laser systems, which complements the overall accessibility to 54 elements including use of traditional dye lasers. Recent progress in the field concerns the identification of suitable optical excitation schemes for Ti:Sapphire laser excitation as well as technical developments of the source in respect to geometry, cavity material as well as by incorporation of an ion guide system in the form of the laser ion source trap LIST.

Organic Lasers: Recent Developments on Materials, Device Geometries, and Fabrication Techniques.

PubMed

Kuehne, Alexander J C; Gather, Malte C

2016-11-09

Organic dyes have been used as gain medium for lasers since the 1960s, long before the advent of today's organic electronic devices. Organic gain materials are highly attractive for lasing due to their chemical tunability and large stimulated emission cross section. While the traditional dye laser has been largely replaced by solid-state lasers, a number of new and miniaturized organic lasers have emerged that hold great potential for lab-on-chip applications, biointegration, low-cost sensing and related areas, which benefit from the unique properties of organic gain materials. On the fundamental level, these include high exciton binding energy, low refractive index (compared to inorganic semiconductors), and ease of spectral and chemical tuning. On a technological level, mechanical flexibility and compatibility with simple processing techniques such as printing, roll-to-roll, self-assembly, and soft-lithography are most relevant. Here, the authors provide a comprehensive review of the developments in the field over the past decade, discussing recent advances in organic gain materials, which are today often based on solid-state organic semiconductors, as well as optical feedback structures, and device fabrication. Recent efforts toward continuous wave operation and electrical pumping of solid-state organic lasers are reviewed, and new device concepts and emerging applications are summarized.

Application of PDT for Uterine Cervical Cancer

PubMed Central

Kawasaki, K.; Suehiro, Y.; Kunugi, T.; Umayahara, K.; Akiya, T.; Iwabuchi, H.; Sakunaga, H.; Sakamoto, M.; Sugishita, T.; Tenjin, Y.

1999-01-01

We have been performing PDT using Excimer Dye Laser (EDL) or YAG-OPO laser, a type of low power laser, both of which have a considerably higher degree of tissue penetration even when compared to PDT using Argon Dye Laser (ADL). PDT is a relatively simple procedure without any bleeding and does not require anesthesia since it causes no pain. PDT is performed 48 h after intravenous injection of 1.5–2.0 mg/kg of PHE (Photofrin®). Precise spot irradiation is possible using a colposcope with an optical laser path. We also use a cervical probe which enables photoirradiation of the entire cervical canal. We have performed PDT on 131 cases (95 CIS, 31 dysplasia, 1 vulval dysplasia (VIN), 3 squamous cell carcinoma, microinvasion, and 1 CIS + endocervical adenocarcinoma, microinvasion). Of these cases, 127 became CR (96.9%). The first CR case was 10 years ago and no recurrence has been observed yet. PDT is extremely effective to preserve fertility. Except for sensitive reactions to sunlight, there are no noticeable side effects or difficulties related to pregnancy or delivery. We expect that in the near future PDT will be performed using diode lasers and without hospitalization due to new photosensitizers which have shorter retention times. PMID:18493501

Intense excitation source of blue-green laser

NASA Astrophysics Data System (ADS)

Han, Kwang S.

1986-10-01

An intense and efficient source for blue green laser useful for the space-based satellite laser applications, underwater strategic communication, and measurement of ocean bottom profile is being developed. The source in use, the hypocycloidal pinch plasma (HCP), and the dense plasma focus (DPF) can produce intense uv photons (200 to 400nm) which match the absorption spectra of both near UV and blue green dye lasers (300 to 400nm). As a result of optimization of the DPF light at 355nm, the blue green dye (LD490) laser output exceeding 4mJ was obtained at the best cavity tunning of the laser system. With the HCP pumped system a significant enhancement of the blue green laser outputs with dye LD490 and coumarin 503 has been achieved through the spectrum conversion of the pumping light by mixing a converter dye BBQ. The maximum increase of laser output with the dye mixture of LD490+BBQ and coumarin 503+BBQ was greater than 80%. In addition, the untunned near UV lasers were also obtained. The near UV laser output energy of P-terphenyl dye was 0.5mJ at lambda sub C=337nm with the bandwidth of 3n m for the pulse duration of 0.2us. Another near UV laser output energy obtained with BBQ dye was 25 mJ at lambda sub C=383nm with the bandwidth of 3nm for the pulse duration of 0.2us. Another near UV laser output energy obtained with BBQ dye was 25 mJ at lambda sub C=383nm with the bandwidth of 3nm for the pulse duration of 0.2microsec.

Integrated optical components in thin films of polymers

NASA Technical Reports Server (NTRS)

Sarkisov, Sergey; Abdeldayem, Hossin; Venkateswarlu, Putcha; Teague, Zedric

1995-01-01

The results will be reported on the study of integrated optical components based on nonlinear optical polymeric films. Polymers poly(methyl methacrylate) (PMMA) and polyimide (PI) doped with organic laser dyes 4-dicyanomethylene-2-methyl-6-p dimethylaminostyryl-4H pyran (DCM) and 1, 3, 5, 7, 8 - pentamethyl-2,6 -diethyl-pyrromethene -BF2-complex (Pyrommethene 567, PM-567) were selected as materials for light guiding films. Additionally, UV polymerized polydiacetylene (PDA) on glass substrate was used as a waveguide material. Optical waveguides were fabricated using spin coating of preoxidized silicon wafers (1.5 micrometer silicon oxide layer) with organic dye/polymer solution followed by soft baking. the modes in slab waveguides were studied using prism coupling techniques. Measured values of mode coupling angles in multimode waveguides were used to calculate film thickness and refractive index for different polarizations. Refractive index anisotropy was found in PDA waveguide. The optimal conditions of spin coating for single mode waveguide fabrication were estimated. Propagation losses were measured by collecting the light scattered from the trace of a propagating mode either by scanning photo detector or by CCD camera. Different types of light coupling techniques were used including end-dire coupling, prism and grating coupling. Mechanical printing technique was developed for coupling grating fabrication resulting in gratings with 4% diffraction efficiency. The gratings demonstrated good stability with diffraction efficiency relaxation rate 2.4 dB/hour at a temperature approximately 15-20 C below glass transition point. Dye doped waveguides were transversally pumped with frequency doubled Nd:YAG Q-switched laser producing intensive light emission with apparent 6 kW/sq cm pump threshold and spectrum narrowing near 617 nm peak in the case of DCM doped waveguide. PM-567 doped waveguide pumped with CW Ar(+) laser (514 nm wavelength) far below threshold (0.1 W/sq.cm pump power) demonstrated emission spectrum narrowing near 616 nm peak with 18% power conversion slope efficiency. In this case emission spectrum modification was caused by the enhanced light absorption along the direction of propagating waveguide modes. Changing length, thickness, and other morphlogical waveguide parameters one can modify emission spectrum in predictable direction. The results show that polymeric waveguides, especially based on high temperature polymers such as Pl, can be used to produce a varietiy of active and passive silicon compatible integrated optical components for aerospace applications.

Optical measurement of acidification of human dental plaque in vitro

NASA Astrophysics Data System (ADS)

Graham, Jasmine Y.; Nelson, Leonard Y.; Seibel, Eric J.

2018-02-01

A pH measurement of oral biofilms is helpful for monitoring the impact of acidogenic bacteria in the caries process. Demineralization of dental enamel is closely related to the time dependent pH of human plaque. Therefore, providing a means to easily measure the local pH of biofilms is a useful clinical diagnostic in the arsenal of caries prevention tools. Optical measurement methods of plaque metabolism can use intrinsic fluorescence or extrinsic fluorescence from added dyes. Autofluorescence spectral features of human oral biofilms at green (500 nm) and red (634 nm) fluorescence wavelengths using 405 nm excitation did not demonstrate a spectral or intensity shift between neutral and acidic conditions. Chlorin e6, an ingredient in chlorophyllin food supplement, exhibited a spectral and intensity shift of fluorescence emission in buffered solutions, but this quantitative pH-dependence was not transferable to a human plaque environment. Finally, a ratiometric quantitative pH measure was achieved by exciting (405 nm laser) a mixture of two dyes, fluorescein and rhodamine B. This two-dye mixture produced two strong fluorescent bands centered at 515 nm (fluorescein) and 580 nm (rhodamine B), where the 515 nm band was pH sensitive and the 580 nm band served as a pH insensitive reference. This dual-dye fluorescence ratio exhibited a linear response over pH 7 to 5 in human oral biofilms during a sugar challenge. We have explored methods to use non-contact, optical measures of local acidity levels in difficult to access dental locations such as occlusal fissures using various pH sensitive fluorescent dye systems.

Simultaneous measurements of absorption spectrum and refractive index in a microfluidic system.

PubMed

Helseth, Lars Egil

2012-02-13

The characterization of dyes in various solvents requires determination of the absorption spectrum of the dye as well as the refractive index of the solvent. Typically, the refractive index of the solvent and the absorption spectrum of the solute are measured using separate experimental setups where significant liquid volumes are required. In this work the first optical measurement system that is able to do simultaneous measurements of the refractive index of the solvent and the spectral properties of the solute in a microscopic volume is presented. The laser dye Rhodamine 6G in glycerol is investigated, and the refractive index of the solution is monitored using the interference pattern of the light scattered off the channel, while its spectral properties is found by monitoring reflected light from the channel.

Four generations of sodium guide star lasers for adaptive optics in astronomy and space situational awareness

NASA Astrophysics Data System (ADS)

d'Orgeville, Céline; Fetzer, Gregory J.

2016-07-01

This paper recalls the history of sodium guide star laser systems used in astronomy and space situational awareness adaptive optics, analyzing the impact that sodium laser technology evolution has had on routine telescope operations. While it would not be practical to describe every single sodium guide star laser system developed to date, it is possible to characterize their evolution in broad technology terms. The first generation of sodium lasers used dye laser technology to create the first sodium laser guide stars in Hawaii, California, and Spain in the late 1980s and 1990s. These experimental systems were turned into the first laser guide star facilities to equip mediumto- large diameter adaptive optics telescopes, opening a new era of Laser Guide Star Adaptive Optics (LGS AO)-enabled diffraction-limited imaging from the ground. Although they produced exciting scientific results, these laser guide star facilities were large, power-hungry and messy. In the USA, a second-generation of sodium lasers was developed in the 2000s that used cleaner, yet still large and complex, solid-state laser technology. These are the systems in routine operation at the 8 to 10m-class astronomical telescopes and 4m-class satellite imaging facilities today. Meanwhile in Europe, a third generation of sodium lasers was being developed using inherently compact and efficient fiber laser technology, and resulting in the only commercially available sodium guide star laser system to date. Fiber-based sodium lasers are being or will soon be deployed at three astronomical telescopes and two space surveillance stations. These highly promising systems are still relatively large to install on telescopes and they remain significantly expensive to procure and maintain. We are thus proposing to develop a fourth generation of sodium lasers: based on semiconductor technology, these lasers could provide a definitive solution to the problem of sodium LGS AO laser sources for all astronomy and space situational awareness applications.

Excimer Pumped Pulsed Tunable Dye Laser

NASA Astrophysics Data System (ADS)

Littman, Michael G.

1988-06-01

It has been recently shown and reported for the first time at this meeting, that Excimer pumping of a single-mode, short-cavity, grazing-incidence, longitudinally-pumped pulsed dye laser is feasible. In this paper the key concepts upon which this latest development is based are presented and are in a somewhat unusual form. This manuscript describes five specific dye laser examples. The five examples represent a progression from the simplest type of dye laser to the single-mode version mentioned above. The examples thus serve as a tutorial introduction to potential users of dye lasers. The article is organized into five sections or STEPS, each of which describes a different pulsed dye laser. Since the subtle points about dye lasers are best appreciated only after one actually attempts to build a working model, a PROCEDURES category is included in which details about the construction of the particular form of laser are given. As one reads through this category, think of it as looking over the shoulder of the laser builder. The NOTES category which follows is a brief but essential discussion explaining why various components and procedures are used, as well as how laser performance specifications are obtained. This subsection can he viewed as a discussion with the laser builder concerning the reasons for specific actions and choices made in the assembly of the example laser. The last category contains COMMENTS which provide additional related information pertaining to the example laser that goes beyond the earlier annotated discussion. If you like, these are the narrator's comments. At the end of the article, after the five sequential forms of the laser have been presented, there is a brief summation.

Identification Of Natural Dyes On Archaeological Textile Objects Using Laser Induced Fluorescent Technique

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abdel-Kareem, O.; Eltokhy, A.; Harith, M. A.

2011-09-22

This study aims to evaluate the use of Laser Fluorescent as a non-destructive technique for identification of natural dyes on archaeological textile objects. In this study wool textile samples were dyed with 10 natural dyes such as cochineal, cutch, henna, indigo, Lac, madder, safflower, saffron, sumac and turmeric. These dyes common present on archaeological textile objects to be used as standard dyed textile samples. These selected natural dyes will be used as known references that can be used a guide to identify unknown archaeological dyes. The dyed textile samples were investigated with laser radiation in different wavelengths to detect themore » best wavelengths for identification each dye. This study confirms that Laser Florescent is very useful and a rapid technique can be used as a non-destructive technique for identification of natural dyes on archaeological textile objects. The results obtained with this study can be a guide for all conservators in identification of natural organic dyes on archaeological textile objects.« less

Identification Of Natural Dyes On Archaeological Textile Objects Using Laser Induced Fluorescent Technique

NASA Astrophysics Data System (ADS)

Abdel-Kareem, O.; Eltokhy, A.; Harith, M. A.

2011-09-01

This study aims to evaluate the use of Laser Fluorescent as a non-destructive technique for identification of natural dyes on archaeological textile objects. In this study wool textile samples were dyed with 10 natural dyes such as cochineal, cutch, henna, indigo, Lac, madder, safflower, saffron, sumac and turmeric. These dyes common present on archaeological textile objects to be used as standard dyed textile samples. These selected natural dyes will be used as known references that can be used a guide to identify unknown archaeological dyes. The dyed textile samples were investigated with laser radiation in different wavelengths to detect the best wavelengths for identification each dye. This study confirms that Laser Florescent is very useful and a rapid technique can be used as a non-destructive technique for identification of natural dyes on archaeological textile objects. The results obtained with this study can be a guide for all conservators in identification of natural organic dyes on archaeological textile objects.

Detection of Single Molecules Illuminated by a Light-Emitting Diode

PubMed Central

Gerhardt, Ilja; Mai, Lijian; Lamas-Linares, Antía; Kurtsiefer, Christian

2011-01-01

Optical detection and spectroscopy of single molecules has become an indispensable tool in biological imaging and sensing. Its success is based on fluorescence of organic dye molecules under carefully engineered laser illumination. In this paper we demonstrate optical detection of single molecules on a wide-field microscope with an illumination based on a commercially available, green light-emitting diode. The results are directly compared with laser illumination in the same experimental configuration. The setup and the limiting factors, such as light transfer to the sample, spectral filtering and the resulting signal-to-noise ratio are discussed. A theoretical and an experimental approach to estimate these parameters are presented. The results can be adapted to other single emitter and illumination schemes. PMID:22346610

Threshold pump power of a solar-pumped dye laser

NASA Technical Reports Server (NTRS)

Lee, Ja H.; Kim, Kyung C.; Kim, Kyong H.

1988-01-01

Threshold solar power for dye laser pumping has been determined by measuring the gain of a rhodamine 6G dye laser amplifier at various solar-simulated irradiances on an amplifier cell. The measured threshold was 20,000 solar constants (2.7 kW/sq cm) for the dye volume of 2 x 5 x 40 cu mm and the optimum dye concentration of 0.001 M. The threshold is about one-third of that achievable with a high-intensity solar concentrator.

Photodegradation and Photophysics of Laser Dyes

DTIC Science & Technology

1994-06-30

research. "The Photophysics and Photochem istry of’ Orgainic Laser Dyecs uander Conditions oit Binding to Polymethacrylic Acid in Water** thcsis...c 13. ABSTRACT (Maximum 200 wotrds) 6 The solubilization of laser dyes in water with the aid of the polyelectrolyte, poly(methacr,-- lic acid ) (PMAA...moderately acidic pH. Polymer-bound dyes in water display markedly enhanced emission yield, lifetime, and polarization. Dye materials are also less

Investigation of potential of differential absorption Lidar techniques for remote sensing of atmospheric pollutants

NASA Technical Reports Server (NTRS)

Butler, C. F.; Shipley, S. T.; Allen, R. J.

1981-01-01

The NASA multipurpose differential absorption lidar (DIAL) system uses two high conversion efficiency dye lasers which are optically pumped by two frequency-doubled Nd:YAG lasers mounted rigidly on a supporting structure that also contains the transmitter, receiver, and data system. The DIAL system hardware design and data acquisition system are described. Timing diagrams, logic diagrams, and schematics, and the theory of operation of the control electronics are presented. Success in obtaining remote measurements of ozone profiles with an airborne systems is reported and results are analyzed.

Visualization of Pulmonary Clearance Mechanisms via Noninvasive Optical Imaging Validated by Near-Infrared Flow Cytometry

PubMed Central

Zhou, Haiying; Gunsten, Sean P.; Zhegalova, Natalia G.; Bloch, Sharon; Achilefu, Samuel; Holley, J. Christopher; Schweppe, Daniel; Akers, Walter; Brody, Steven L.; Eades, William; Berezin, Mikhail Y.

2016-01-01

In vivo optical imaging with near-infrared (NIR) probes is an established method of diagnostics in preclinical and clinical studies. However, the specificities of these probes are difficult to validate ex vivo due to the lack of NIR flow cytometry. To address this limitation, we modified a flow cytometer to include an additional NIR channel using a 752 nm laser line. The flow cytometry system was tested using NIR microspheres and cell lines labeled with a combination of visible range and NIR fluorescent dyes. The approach was verified in vivo in mice evaluated for immune response in lungs after intratracheal delivery of the NIR contrast agent. Flow cytometry of cells obtained from the lung bronchoalveolar lavage demonstrated that the NIR dye was taken up by pulmonary macrophages as early as four-hours post-injection. This combination of optical imaging with NIR flow cytometry extends the capability of imaging and enables complementation of in vivo imaging with cell-specific studies. PMID:25808737

Optical tweezers and non-ratiometric fluorescent-dye-based studies of respiration in sperm mitochondria

NASA Astrophysics Data System (ADS)

Chen, Timothy; Shi, Linda Z.; Zhu, Qingyuan; Chandsawangbhuwana, Charlie; Berns, Michael W.

2011-04-01

The purpose of this study is to investigate how the mitochondrial membrane potential affects sperm motility using laser tweezers and a non-ratiometric fluorescent probe, DiOC6(3). A 1064 nm Nd:YVO4 continuous wave laser was used to trap motile sperm at a power of 450 mW in the trap spot. Using customized tracking software, the curvilinear velocity (VCL) and the escape force from the laser tweezers were measured. Human (Homo sapiens), dog (Canis lupis familiaris) and drill (Mandrillus leucophaeus) sperm were treated with DiOC6(3) to measure the membrane potential in the mitochondria-rich sperm midpieces. Sperm from all three species exhibited an increase in fluorescence when treated with the DiOC6(3). When a cyanide inhibitor (CCCP) of aerobic respiration was applied, sperm of all three species exhibited a reduction in fluorescence to pre-dye levels. With respect to VCL and escape force, the CCCP had no effect on dog or human sperm, suggesting a major reliance upon anaerobic respiration (glycolysis) for ATP in these two species. Based on the preliminary study on drill sperm, CCCP caused a drop in the VCL, suggesting potential reliance on both glycolysis and aerobic respiration for motility. The results demonstrate that optical trapping in combination with DiOC6(3) is an effective way to study sperm motility and energetics.

Compact Ozone Differential Absorption Lidar (DIAL) Transmitter Using Solid-State Dye Polymers

NASA Technical Reports Server (NTRS)

Jones, Alton L., Jr.; DeYoung, Russell J.; Elsayid-Ele, Hani

2001-01-01

A new potential DIAL laser transmitter is described that uses solid-state dye laser materials to make a simpler, more compact, lower mass laser system. Two solid-state dye laser materials were tested to evaluate their performance in a laser oscillator cavity end pumped by a pulsed Nd:YAG laser at 532 nm. The polymer host polymethyl-methacrylate was injected with a pyrromethene laser dye, PM 580, or PM 597. A narrowband laser oscillator cavity was constructed to produce visible wavelengths of 578 and 600 nm which were frequency doubled into the UV region (299 or 300 nm) by using a BBO crystal, resulting in a maximum energy of 11 mJ at a wavelength of 578 nm when pumped by the Nd:YAG laser at an energy of 100 mJ (532 nm). A maximum output energy of 378 microJ was achieved in the UV region at a wavelength of 289 nm but lasted only 2000 laser shots at a repetition rate of 10 Hz. The results are promising and show that a solid-state dye laser based ozone DIAL system is possible with improvements in the design of the laser transmitter.

Laser Radar Study Using Resonance Absorption for Remote Detection Of Air Pollutants

NASA Technical Reports Server (NTRS)

Igarashi, Takashi

1973-01-01

A laser radar using resonance absorption has an advantage of increased detection range and sensitivity compared with that achieved by Raman or resonance back scattering. In this paper, new laser radar system using resonance absorption is proposed and results obtained from this laser radar system are discussed. NO2, SO2 gas has an absorption spectrum at 4500 A and 3000 A respectively as shown in Fig. 1. A laser light including at least a set of an absorption peak (lambda)1 and a valley (lambda)2 is emitted into a pollutant atmosphere. The light reflected with a topographical reflector or an atmospheric Mie scattering as distributed reflectors is received and divided into two wavelength components (lambda)1 and (lambda)2. The laser radar system used in the investigation is shown in Fig', 2 and consists of a dye laser transmitter, an optical receiver with a special monochrometer and a digital processer. Table 1 shows the molecular constants of NO2, and SO2 and the dye laser used in this experiment. In this system, the absolute concentration of the pollutant gas can be measured in comparison with a standard gas cell. The concentration of NO2, SO2 as low as 0.1 ppm have been measured at 100 m depth resolution. For a 1 mJ laser output, the observable range of this system achieved up to 300 m using the distributed Mie reflector. The capability and technical limitation of the system will be discussed in detail.

Direct measurement of optoacoustic induced ultrasonic waves

NASA Astrophysics Data System (ADS)

Kuo, C.-Y.; Patel, C. K. N.

1984-04-01

A two-beam optically induced photoacoustic grating and its low frequency ultrasonic waves are measured with a thin film transducer in low concentration dye solution. The acoustic power generated is measured for a weakly absorbing medium to be equal to about 5 W sq cm, indicating high conversion efficiency. Application of this direct detection as a laser beam profiler is also discussed.

Femtosecond Optoinjection of Intact Tobacco BY-2 Cells Using a Reconfigurable Photoporation Platform

PubMed Central

Mitchell, Claire A.; Kalies, Stefan; Cizmár, Tomás; Heisterkamp, Alexander; Torrance, Lesley; Roberts, Alison G.; Gunn-Moore, Frank J.; Dholakia, Kishan

2013-01-01

A tightly-focused ultrashort pulsed laser beam incident upon a cell membrane has previously been shown to transiently increase cell membrane permeability while maintaining the viability of the cell, a technique known as photoporation. This permeability can be used to aid the passage of membrane-impermeable biologically-relevant substances such as dyes, proteins and nucleic acids into the cell. Ultrashort-pulsed lasers have proven to be indispensable for photoporating mammalian cells but they have rarely been applied to plant cells due to their larger sizes and rigid and thick cell walls, which significantly hinders the intracellular delivery of exogenous substances. Here we demonstrate and quantify femtosecond optical injection of membrane impermeable dyes into intact BY-2 tobacco plant cells growing in culture, investigating both optical and biological parameters. Specifically, we show that the long axial extent of a propagation invariant (“diffraction-free”) Bessel beam, which relaxes the requirements for tight focusing on the cell membrane, outperforms a standard Gaussian photoporation beam, achieving up to 70% optoinjection efficiency. Studies on the osmotic effects of culture media show that a hypertonic extracellular medium was found to be necessary to reduce turgor pressure and facilitate molecular entry into the cells. PMID:24244456

Femtosecond optoinjection of intact tobacco BY-2 cells using a reconfigurable photoporation platform.

PubMed

Mitchell, Claire A; Kalies, Stefan; Cizmár, Tomás; Heisterkamp, Alexander; Torrance, Lesley; Roberts, Alison G; Gunn-Moore, Frank J; Dholakia, Kishan

2013-01-01

A tightly-focused ultrashort pulsed laser beam incident upon a cell membrane has previously been shown to transiently increase cell membrane permeability while maintaining the viability of the cell, a technique known as photoporation. This permeability can be used to aid the passage of membrane-impermeable biologically-relevant substances such as dyes, proteins and nucleic acids into the cell. Ultrashort-pulsed lasers have proven to be indispensable for photoporating mammalian cells but they have rarely been applied to plant cells due to their larger sizes and rigid and thick cell walls, which significantly hinders the intracellular delivery of exogenous substances. Here we demonstrate and quantify femtosecond optical injection of membrane impermeable dyes into intact BY-2 tobacco plant cells growing in culture, investigating both optical and biological parameters. Specifically, we show that the long axial extent of a propagation invariant ("diffraction-free") Bessel beam, which relaxes the requirements for tight focusing on the cell membrane, outperforms a standard Gaussian photoporation beam, achieving up to 70% optoinjection efficiency. Studies on the osmotic effects of culture media show that a hypertonic extracellular medium was found to be necessary to reduce turgor pressure and facilitate molecular entry into the cells.

Construction and Characterization of External Cavity Diode Lasers for Atomic Physics

PubMed Central

Hardman, Kyle S.; Bennetts, Shayne; Debs, John E.; Kuhn, Carlos C. N.; McDonald, Gordon D.; Robins, Nick

2014-01-01

Since their development in the late 1980s, cheap, reliable external cavity diode lasers (ECDLs) have replaced complex and expensive traditional dye and Titanium Sapphire lasers as the workhorse laser of atomic physics labs1,2. Their versatility and prolific use throughout atomic physics in applications such as absorption spectroscopy and laser cooling1,2 makes it imperative for incoming students to gain a firm practical understanding of these lasers. This publication builds upon the seminal work by Wieman3, updating components, and providing a video tutorial. The setup, frequency locking and performance characterization of an ECDL will be described. Discussion of component selection and proper mounting of both diodes and gratings, the factors affecting mode selection within the cavity, proper alignment for optimal external feedback, optics setup for coarse and fine frequency sensitive measurements, a brief overview of laser locking techniques, and laser linewidth measurements are included. PMID:24796259

Construction and characterization of external cavity diode lasers for atomic physics.

PubMed

Hardman, Kyle S; Bennetts, Shayne; Debs, John E; Kuhn, Carlos C N; McDonald, Gordon D; Robins, Nick

2014-04-24

Since their development in the late 1980s, cheap, reliable external cavity diode lasers (ECDLs) have replaced complex and expensive traditional dye and Titanium Sapphire lasers as the workhorse laser of atomic physics labs. Their versatility and prolific use throughout atomic physics in applications such as absorption spectroscopy and laser cooling makes it imperative for incoming students to gain a firm practical understanding of these lasers. This publication builds upon the seminal work by Wieman, updating components, and providing a video tutorial. The setup, frequency locking and performance characterization of an ECDL will be described. Discussion of component selection and proper mounting of both diodes and gratings, the factors affecting mode selection within the cavity, proper alignment for optimal external feedback, optics setup for coarse and fine frequency sensitive measurements, a brief overview of laser locking techniques, and laser linewidth measurements are included.

Remote sensing of tropospheric gases and aerosols with airborne DIAL system

NASA Technical Reports Server (NTRS)

Browell, E. V.

1983-01-01

The multipurpose airborne DIAL system developed at NASA Langley Research Center is characterized, and the published results of tropospheric O3, H2O, and aerosol-backscatter remote-sensing experiments performed in 1980 and 1981 are summarized. The system comprises two tunable dye lasers pumped by frequency-doubled Nd:YAG lasers, dielectric-coated steering optics, a 36-cm-diameter Cassegrain receiver telescope, gateable photomultiplier tubes, and a minicomputer data-processing unit for real-time calculation of gas concentrations and backscattering profiles. The transmitted energy of the 100-microsec-separated dye-laser pulses is 40, 80, or 50 mJ/pulse at around 300, 600, or 720-nm wavelength, respectively. Good agreement was found between DIAL-remote-sensed and in-situ H2O and O3 profiles of the lower troposphere and O3 profiles of the tropopause region, and the usefulness of DIAL backscattering measurements in the study of boundary-layer and tropospheric dynamics is demonstrated. The feasibility of DIAL sensing of power-plant or urban plume SO2, of urban-area (or rural-area column-content) NO2, and of temperature and H2O (simultaneously using a third laser) has been suggested by simulation studies.

Nine color eleven parameter immunophenotyping using three laser flow cytometry.

PubMed

Bigos, M; Baumgarth, N; Jager, G C; Herman, O C; Nozaki, T; Stovel, R T; Parks, D R; Herzenberg, L A

1999-05-01

This study describes a three laser flow cytometer, reagents, and software used to simultaneously evaluate nine distinct fluorescent parameters on one cell sample. We compare the quality of data obtained with (1) full software compensation and (2) the use of partial spectral compensation of selected pairs of parameters in analog hardware, in combination with final software compensation. An application characterizing low frequency murine B cell subpopulations is given. The fluorochromes used are: fluorescein (FITC), phycoerythrin (PE), Cy5PE and Cy7PE, excited at 488 nm by an argon laser; Texas Red (TR), allophycocyanin (APC), and Cy7APC excited at 595 nm by a pumped dye laser; and cascade blue (CB) and cascade yellow (CY) excited at 407 nm by a violet-enhanced krypton laser. Custom additions to commercial electronics and an extended optical bench allow the measurement of these nine parameters plus forward and side scatter light signals. We find the use of partial analog compensation reduces the variation in the background staining levels introduced by the compensation process. Novel B cell populations with frequencies below 1% are characterized. Nine color flow cytometry is capable of providing measurements with high information content. The choice of reagent-dye combinations and the ability to compensate in multi-parameter measurement space are crucial to obtaining satisfactory results.

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

PubMed Central

Cole, Russell H.; Gartner, Zev J.; Abate, Adam R.

2016-01-01

Fluorescence assays are the most common readouts used in droplet microfluidics due to their bright signals and fast time response. Applications such as multiplex assays, enzyme evolution, and molecular biology enhanced cell sorting require the detection of two or more colors of fluorescence. Standard multicolor detection systems that couple free space lasers to epifluorescence microscopes are bulky, expensive, and difficult to maintain. In this paper, we describe a scheme to perform multicolor detection by exciting discrete regions of a microfluidic channel with lasers coupled to optical fibers. Emitted light is collected by an optical fiber coupled to a single photodetector. Because the excitation occurs at different spatial locations, the identity of emitted light can be encoded as a temporal shift, eliminating the need for more complicated light filtering schemes. The system has been used to detect droplet populations containing four unique combinations of dyes and to detect sub-nanomolar concentrations of fluorescein. PMID:27214249

Continuous-wave organic dye lasers and methods

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shapira, Ofer; Chua, Song-Liang; Zhen, Bo

2014-09-16

An organic dye laser produces a continuous-wave (cw) output without any moving parts (e.g., without using flowing dye streams or spinning discs of solid-state dye media to prevent photobleaching) and with a pump beam that is stationary with respect to the organic dye medium. The laser's resonant cavity, organic dye medium, and pump beam are configured to excite a lasing transition over a time scale longer than the associated decay lifetimes in the organic dye medium without photobleaching the organic dye medium. Because the organic dye medium does not photobleach when operating in this manner, it may be pumped continuouslymore » so as to emit a cw output beam. In some examples, operation in this manner lowers the lasing threshold (e.g., to only a few Watts per square centimeter), thereby facilitating electrical pumping for cw operation.« less

Emission lifetimes of a fluorescent dye under shock compression

DOE PAGES

Liu, Wei-long; Bassett, Will P.; Christensen, James M.; ...

2015-10-15

The emission lifetimes of rhodamine 6G (R6G), were measured under shock compression to 9.1 GPa, with the dual intent of better understanding molecular photophysics in extreme environments and assessing the usefulness of fluorescence lifetime microscopy to measure spatially-dependent pressure distributions in shocked microstructured media. R6G was studied as free dye dissolved in poly-methyl methacrylate (PMMA), or dye encapsulated in silica microparticles suspended in PMMA. Thin layers of these materials in impedance-matched geometries were subjected to planar single-stage shocks created by laser-driven flyer plates. A synchronized femtosecond laser excited the dye at selected times relative to flyer plate arrival and themore » emission lifetimes were measured with a streak camera. Lifetimes decreased when shocks arrived. The lifetime decrease was attributed to a shock-induced enhancement of R6G nonradiative relaxation. At least part of the relaxation involved shock-enhanced intersystem crossing. For free dye in PMMA, the lifetime decrease during the shock was shown to be a linear function of shock pressure from 0-9 GPa, with a slope of -0.22 ns·GPa -1. Furthermore, the linear relationship makes it simple to convert lifetimes into pressures. Lifetime measurements in shocked microenvironments may be better than emission intensity measurements, since lifetimes are sensitive to the surrounding environment, but insensitive to intensity variations associated with the motion and optical properties of a dynamically changing structure.« less

Multi-wavelength laser from dye-doped cholesteric polymer films.

PubMed

Huang, Yuhua; Wu, Shin-Tson

2010-12-20

A multi-wavelength laser is demonstrated using a dye-doped cholesteric polymer film whose reflection bandwidth is broadened with several oscillations. Due to the abrupt change of the density of state between oscillation peak and valley, each oscillation functions as a photonic band gap for generating a laser wavelength under the excitation of a pumping laser. As a result, a multiple wavelength laser is generated. Results indicate that the dye-doped cholesteric liquid crystal polymer film is a good candidate for fabricating broadband lasers such as white light lasers. Potential applications include experimental testing of laser materials, identification markers, information displays, and inertial confinement laser fusion.

Interface of physics and biology: engineering virus-based nanoparticles for biophotonics.

PubMed

Wen, Amy M; Infusino, Melissa; De Luca, Antonio; Kernan, Daniel L; Czapar, Anna E; Strangi, Giuseppe; Steinmetz, Nicole F

2015-01-21

Virus-based nanoparticles (VNPs) have been used for a wide range of applications, spanning basic materials science and translational medicine. Their propensity to self-assemble into precise structures that offer a three-dimensional scaffold for functionalization has led to their use as optical contrast agents and related biophotonics applications. A number of fluorescently labeled platforms have been developed and their utility in optical imaging demonstrated, yet their optical properties have not been investigated in detail. In this study, two VNPs of varying architectures were compared side-by-side to determine the impact of dye density, dye localization, conjugation chemistry, and microenvironment on the optical properties of the probes. Dyes were attached to icosahedral cowpea mosaic virus (CPMV) and rod-shaped tobacco mosaic virus (TMV) through a range of chemistries to target particular side chains displayed at specific locations around the virus. The fluorescence intensity and lifetime of the particles were determined, first using photochemical experiments on the benchtop, and second in imaging experiments using tissue culture experiments. The virus-based optical probes were found to be extraordinarily robust under ultrashort, pulsed laser light conditions with a significant amount of excitation energy, maintaining structural and chemical stability. The most effective fluorescence output was achieved through dye placement at optimized densities coupled to the exterior surface avoiding conjugated ring systems. Lifetime measurements indicate that fluorescence output depends not only on spacing the fluorophores, but also on dimer stacking and configurational changes leading to radiationless relaxation-and these processes are related to the conjugation chemistry and nanoparticle shape. For biological applications, the particles were also examined in tissue culture, from which it was found that the optical properties differed from those found on the benchtop due to effects from cellular processes and uptake kinetics. Data indicate that fluorescent cargos are released in the endolysosomal compartment of the cell targeted by the virus-based optical probes. These studies provide insight into the optical properties and fates of fluorescent proteinaceous imaging probes. The cellular release of cargo has implications not only for virus-based optical probes, but also for drug delivery and release systems.

Spectroscopic properties and amplified spontaneous emission of fluorescein laser dye in ionic liquids as green media

NASA Astrophysics Data System (ADS)

AL-Aqmar, Dalal M.; Abdelkader, H. I.; Abou Kana, Maram T. H.

2015-09-01

The use of ionic liquids (ILs) as milieu materials for laser dyes is a promising field and quite competitive with volatile organic solvents and solid state-dye laser systems. This paper investigates some photo-physical parameters of fluorescein dye incorporated into ionic liquids; 1-Butyl-3-methylimidazolium chloride (BMIM Cl), 1-Butyl-3-methylimidazolium tetrachloroaluminate (BMIM AlCl4) and 1-Butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) as promising host matrix in addition to ethanol as reference. These parameters are: absorption and emission cross-sections, fluorescence lifetime and quantum yield, in addition to the transition dipole moment, the attenuation length and oscillator strength were also investigated. Lasing characteristics such as amplified spontaneous emission (ASE), the gain, and the photostability of fluorescein laser dye dissolved in different host materials were assessed. The composition and properties of the matrix of ILs were found that it has great interest in optimizing the laser performance and photostability of the investigated laser dye. Under transverse pumping of fluorescein dye by blue laser diode (450 nm) of (400 mW), the initial ASE for dye dissolved in BMIM AlCl4 and ethanol were decreased to 39% and 36% respectively as time progressed 132 min. Relatively high efficiency and high fluorescence quantum yield (11.8% and 0.82% respectively) were obtained with good photostability in case of fluorescein in BMIM BF4 that was decreased to ∼56% of the initial ASE after continuously pumping with 400 mW for 132 min.

Characterization of a liquid crystal/dye cell for a future application in display-integrated photovoltaics

NASA Astrophysics Data System (ADS)

Fujieda, Ichiro; Itaya, Shunsuke; Ohta, Masamichi; Ozawa, Shintaro; Azmi, Nada Dianah Binti M.

2016-04-01

One can convert a luminescent solar concentrator to a display by scanning a laser beam on it. When a guest-host system of liquid crystal (LC) and dye materials are incorporated, absorption of excitation light and the radiation pattern of photoluminescence (PL) can be adjusted to changes in lighting condition. The resolution of a displayed image can be degraded by PL spreading in the LC/dye layer. Its contrast can be limited by the PL induced by ambient light. In the experiment, we fabricated a 22×25 mm2 cell that contained 0.5 wt. % coumarin 6 in a nematic LC host. The alignment was antiparallel and the gap was 6 μm. Using a blue laser beam of 0.04 mm FWHM, the PL intensity distribution was measured to be 0.20 mm FWHM at zero bias. It became slightly wider at 10 V. For contrast evaluation, we measured PL spectra under two conditions. First, the center of the cell was irradiated by a 1.7-mW blue laser beam. Second, the whole cell was uniformly exposed to light from a fluorescent lamp at illuminance of 800lx. The contrast of luminance was calculated to be 1.4×105. The optical power reaching its edge surfaces was measured and roughly agreed with the prediction by a simple model.

Preparation of certain m-aminophenols and the use thereof for preparation of laser dyes

DOEpatents

Hammond, P.R.

1983-12-29

Methods are provided for making certain m-aminophenols using a sulfonation/alkali fusion procedure. The aminophenols are key intermediates in the synthesis of dyes, particularly efficient, stable dyes for laser application. Preparations of some rhodamine and phenoxazone dyes from the m-aminophenols are described.

Preparation of certain m-aminophenols and the use thereof for preparation of laser dyes

DOEpatents

Hammond, Peter R.

1986-01-01

Methods are provided for making certain m-aminophenols using a sulfonation/alkali fusion procedure. The aminophenols are key intermediates in the synthesis of dyes, particularly efficient, stable dyes for laser application. Preparations of some rhodamine and phenoxazone dyes from the m-aminophenols are described.

Invasive leg vein treatment with 1064/1319 Nd:YAG laser: combination with dye laser treatment

NASA Astrophysics Data System (ADS)

Smucler, Roman; Horak, Ladislav; Mazanek, Jiri

1999-06-01

More than 2 500 leg veins patients were treated with dye laser / ScleroPlus, Candela, USA / successfully in our clinic and we use this therapy as the basic cosmetics treatment. But especially diameter of leg vein is limiting factor. Very often we have to treat some cases that are not ideal for classical surgical or for dye laser method. We decided to make invasive perivenous laser coagulation. We adapted original Czech 1064/1319 nm Nd:YAG laser / US patent pending /, which is new combine tool, for invasive application. Principe: After we have penetrated the cutis with laser fiber we coagulate leg veins during slowly perivenous motion. Perfect preoperative examination is a condition of success. After 15 months we have very interesting results. Some patients / 15%/ were perfect treated only with this possibility but excellent results are acquired from combination with dye laser.

Direct solar-pumped iodine laser amplifier

NASA Technical Reports Server (NTRS)

Han, Kwang S.; Kim, K. H.; Stock, L. V.

1986-01-01

In order to evaluate the feasibility of the solar pumped dye laser, the parametric study of a dye laser amplifier pumped by a solar simulator and flashlamp was carried out, and the amplifier gains were measured at various pump beam irradiances on the dye cell. Rhodamine 6G was considered as a candidate for the solar pumped laser because of its good utilization of the solar spectrum and high quantum efficiency. The measurement shows that a solar concentration of 20,000 is required to reach the threshold of the dye. The work to construct a kinetic model algorithm which predicts the output parameter of laser was progressed. The kinetic model was improved such that there is good agreement between the theoretical model and experimental data for the systems defined previously as flashlamp pumped laser oscillator, and the long path length solar pumped laser.

Photophysical parameters and fluorescence quenching of 7-diethylaminocoumarin (DEAC) laser dye

NASA Astrophysics Data System (ADS)

El-Mossalamy, E. H.; Obaid, A. Y.; El-Daly, S. A.

2011-10-01

The optical properties including electronic absorption spectrum, emission spectrum, fluorescence quantum yield, and dipole moment of electronic transition of 7-diethylaminocoumarin (DEAC) laser dye have been measured in different solvents. Both electronic absorption and fluorescence spectra are red shifted as the polarity of the medium increases, indicating that the dipole moment of molecule increases on excitation. The fluorescence quantum yield of DEAC decreases as the polarity of solvent increases, a result of the role of solvent polarity in stabilization of the twisting of the intramolecular charge transfer (TICT) in excited state, which is a non-emissive state, as well as hydrogen bonding with the hetero-atom of dye. The emission spectrum of DEAC has also been measured in cationic (CTAC) and anionic (SDS) micelles, the intensity increases as the concentration of surfactant increases, and an abrupt change in emission intensity is observed at critical micelle concentration (CMC) of surfactant. 2×10 -3 mol dm -3 of DEAC gives laser emission in the blue region on pumping with nitrogen laser ( λex=337.1 nm). The laser parameters such as tuning range, gain coefficient ( α), emission cross section ( σe), and half-life energy have been calculated in different solvents, namely acetone, dioxane , ethanol, and dimethyforamide (DMF). The photoreactivity of DEAC has been studied in CCl 4 at a wavelength of 366 nm. The values of photochemical yield ( ϕc) and rate constant ( k) are determined. The interaction of organic acceptors such as picric acid (PA), tetracyanoethylene (TCNE), and 7,7,8,8-tetracynoquinonedimethane (TCNQ) with DEAC is also studied using fluorescence measurements in acetonitrile (CH 3CN); from fluorescence quenching study we assume the possible electron transfer from excited donor DEAC to organic acceptor forming non-emissive exciplex.

Photodynamic therapy: a synergy between light and colors

NASA Astrophysics Data System (ADS)

Merigo, Elisabetta; Sozzi, Michele; Ciociola, Tecla; Conti, Stefania; Fornaini, Carlo; Vescovi, Paolo; Selleri, Stefano; Cucinotta, Annamaria

2015-02-01

In this work the application of different laser wavelengths, in combination with different photosensitizing dyes, to bacterial cultures, in liquid or solid mean, has been investigated. Two types of Streptococcus mutans cultures have been used for the experiments, inside agar and saline solution. Three different laser wavelengths have been applied to the bacterial cultures together with a photosensitizing dye: red diode (650 nm) on cultures stained with Toluidine Blue, blueviolet diode (450 nm) on cultures stained with Curcumin and KTP laser (532 nm) on cultures stained with Erythrosine. The choice of the dye has been made considering the color affinity with the used wavelength. Tests without dyes have also been performed. Experimental results show that the maximum inhibition of bacterial growth with the blue laser has been obtained in a saline solution with a growth of 40.77%. While the combination with Curcumin lead to an inhibition growth of about 99.1%, for a laser fluence of 30J/cm2. No inhibition has been observed using the red laser in saline solution without dye, while the combination with Toluidine Blue resulted in a 100% inhibition growth for 20 and 30 J/cm2 fluences. An inhibition growth of just 16.26% has been obtained with the use of KTP laser in saline solution without dye. The use of Erythrosine had the effect of a complete inhibition growth. From the obtained results it is possible to observe that the combination of laser wavelength with a particular photosensitizing dye can dramatically increase the bacterial growth.

Laser and acoustic lens for lithotripsy

DOEpatents

Visuri, Steven R.; Makarewicz, Anthony J.; London, Richard A.; Benett, William J.; Krulevitch, Peter; Da Silva, Luiz B.

2002-01-01

An acoustic focusing device whose acoustic waves are generated by laser radiation through an optical fiber. The acoustic energy is capable of efficient destruction of renal and biliary calculi and deliverable to the site of the calculi via an endoscopic procedure. The device includes a transducer tip attached to the distal end of an optical fiber through which laser energy is directed. The transducer tip encapsulates an exogenous absorbing dye. Under proper irradiation conditions (high absorbed energy density, short pulse duration) a stress wave is produced via thermoelastic expansion of the absorber for the destruction of the calculi. The transducer tip can be configured into an acoustic lens such that the transmitted acoustic wave is shaped or focused. Also, compressive stress waves can be reflected off a high density/low density interface to invert the compressive wave into a tensile stress wave, and tensile stresses may be more effective in some instances in disrupting material as most materials are weaker in tension than compression. Estimations indicate that stress amplitudes provided by this device can be magnified more than 100 times, greatly improving the efficiency of optical energy for targeted material destruction.

Threshold effect under nonlinear limitation of the intensity of high-power light

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tereshchenko, S A; Podgaetskii, V M; Gerasimenko, A Yu

2015-04-30

A model is proposed to describe the properties of limiters of high-power laser radiation, which takes into account the threshold character of nonlinear interaction of radiation with the working medium of the limiter. The generally accepted non-threshold model is a particular case of the threshold model if the threshold radiation intensity is zero. Experimental z-scan data are used to determine the nonlinear optical characteristics of media with carbon nanotubes, polymethine and pyran dyes, zinc selenide, porphyrin-graphene and fullerene-graphene. A threshold effect of nonlinear interaction between laser radiation and some of investigated working media of limiters is revealed. It is shownmore » that the threshold model more adequately describes experimental z-scan data. (nonlinear optical phenomena)« less

KrF laser-induced OH fluorescence imaging in a supersonic combustion tunnel

NASA Technical Reports Server (NTRS)

Quagliaroli, T. M.; Laufer, G.; Hollo, S. D.; Krauss, R. H.; Whitehurst, R. B., III; Mcdaniel, J. C., Jr.

1992-01-01

Planar fluorescence images of OH in a continuous-flow, electrical-resistively heated, high enthalpy, hydrogen-air combustion tunnel, induced by a tunable KrF laser, were recorded. These images were compared to previously recorded fluorescence images induced by a doubled-dye laser under similar conditions. Images induced by the doubled-dye laser system demonstrated a severe distortion caused by absorption and fluorescence trapping. By contrast, images of the fluorescence induced by the tunable KrF laser retained the symmetry properties of the flow. Based on signal-to-noise ratio measurements the yield of the fluorescence induced by the doubled-dye laser is larger than the fluorescence yield induced by the KrF laser. The measurements in the present facility of OH fluorescence induced by the KrF laser were limited by the photon-statistical noise. Based 2 on this result, doubled-dye laser systems are recommended for OH imaging in small and OH lean (less than 10 exp 15/cu cm) facilities. KrF lasers should be selected otherwise.

Study of the effects of MeV Ag, Cu, Au, and Sn implantation on the optical properties of LiNbO3

NASA Technical Reports Server (NTRS)

Williams, E. K.; Ila, D.; Sarkisov, S.; Curley, M.; Poker, D. B.; Hensley, D. K.; Borel, C.

1998-01-01

The authors present the results of characterization of linear absorption and nonlinear refractive index of Au, Ag, Cu and Sn ion implantation into LiNbO3. Ag was implanted at 1.5 MeV to fluences of 2 to 17 x 17(exp 16)/sq cm at room temperature. Au and Cu were implanted to fluences of 5 to 20 x 10(exp 16)/sq cm at an energy of 2.0 MeV. Sn was implanted to a fluence of 1.6 x 10(exp 17)/sq cm at 160 kV. Optical absorption spectrometry indicated an absorption peak for the Au implanted samples after heat treatment at 1,000 C at approx. 620 nm. The Ag implanted samples absorption peaks shifted from approx. 450 nm before heat treatment to 550 nm after 500 C for 1h. Heat treatment at 800 C returned the Ag implanted crystals to a clear state. Cu nanocluster absorption peaks disappears at 500 C. No Sn clusters were observed by optical absorption or XRD. The size of the Ag and Au clusters as a function of heat treatment were determined from the absorption peaks. The Ag clusters did not change appreciably in size with heat treatment. The Au clusters increased from 3 to 9 nm diameter upon heat treatment at 1000 C. TEM analysis performed on a Au implanted crystal indicated the formation of Au nanocrystals with facets normal to the c-axis. Measurements of the nonlinear refractive indices were carried out using the Z-scan method with a tunable dye laser pumped by a frequency doubled mode-locked Nd:YAG laser. The dye laser had a 4.5 ps pulse duration time and 76 MHz pulse repetition rate (575 nm).

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Wei-long; Bassett, Will P.; Christensen, James M.

The emission lifetimes of rhodamine 6G (R6G), were measured under shock compression to 9.1 GPa, with the dual intent of better understanding molecular photophysics in extreme environments and assessing the usefulness of fluorescence lifetime microscopy to measure spatially-dependent pressure distributions in shocked microstructured media. R6G was studied as free dye dissolved in poly-methyl methacrylate (PMMA), or dye encapsulated in silica microparticles suspended in PMMA. Thin layers of these materials in impedance-matched geometries were subjected to planar single-stage shocks created by laser-driven flyer plates. A synchronized femtosecond laser excited the dye at selected times relative to flyer plate arrival and themore » emission lifetimes were measured with a streak camera. Lifetimes decreased when shocks arrived. The lifetime decrease was attributed to a shock-induced enhancement of R6G nonradiative relaxation. At least part of the relaxation involved shock-enhanced intersystem crossing. For free dye in PMMA, the lifetime decrease during the shock was shown to be a linear function of shock pressure from 0-9 GPa, with a slope of -0.22 ns·GPa -1. Furthermore, the linear relationship makes it simple to convert lifetimes into pressures. Lifetime measurements in shocked microenvironments may be better than emission intensity measurements, since lifetimes are sensitive to the surrounding environment, but insensitive to intensity variations associated with the motion and optical properties of a dynamically changing structure.« less

Carbachol-induced fluid movement through methazolamide-sensitive bicarbonate production in rat parotid intralobular ducts: quantitative analysis of fluorescence images using fluorescent dye sulforhodamine under a confocal laser scanning microscope.

PubMed

Nakamoto, Tetsuji; Shiba, Yoshiki; Hirono, Chikara; Sugita, Makoto; Takemoto, Kazuhisa; Iwasa, Yoshiko; Akagawa, Yasumasa

2002-09-01

Fluid secretion is observed at the openings of ducts in the exocrine gland. It remains unclear whether the ducts are involved in fluid secretion in the salivary glands. In the present study, we investigated the exclusion of fluorescent dye from the duct lumen by carbachol (CCh) in isolated parotid intralobular duct segments to clarify the ability of the ducts for the fluid secretion. When the membrane-impermeable fluorescent dye, sulforhodamine, was added to the superfused extracellular solution, quantitative fluorescence images of the duct lumen were obtained under the optical sectioning at the level of the duct lumen using a confocal laser scanning microscope. CCh decreased the fluorescent intensity in the duct lumen during the superfusion of the fluorescent dye, and CCh flushed out small viscous substances stained with the fluorescent dye from isolated duct lumen, suggesting that CCh might induce fluid secretion in the duct, leading to the clearance of the dye and small stained clumps from the duct lumen. CCh-induced clearance of the fluorescent dye was divided into two phases by the sensitivity to external Ca2+ and methazolamide, an inhibitor for carbonic anhydrase. The initial phase was insensitive to these, and the subsequent late phase was sensitive to these. A major portion in the late phase was inhibited by removal of bicarbonate in the superfusion solution and DPC, but not low concentration of external Cl-, bumetanide or DIDS, suggesting that methazolamide-sensitive production of HCO3-, but not the Cl- uptake mechanism, might contribute to the CCh-induced clearance of the dye from the duct lumen. These results represent the first measurements of fluid movement in isolated duct segments, and suggest that carbachol might evoke fluid secretion possibly through Ca2+-activated, DPC-sensitive anion channels with HCO3- secretion in the rat parotid intralobular ducts.

Clean Photothermal Heating and Controlled Release from Near-Infrared Dye Doped Nanoparticles without Oxygen Photosensitization.

PubMed

Guha, Samit; Shaw, Scott K; Spence, Graeme T; Roland, Felicia M; Smith, Bradley D

2015-07-21

The photothermal heating and release properties of biocompatible organic nanoparticles, doped with a near-infrared croconaine (Croc) dye, were compared with analogous nanoparticles doped with the common near-infrared dyes ICG and IR780. Separate formulations of lipid-polymer hybrid nanoparticles and liposomes, each containing Croc dye, absorbed strongly at 808 nm and generated clean laser-induced heating (no production of (1)O2 and no photobleaching of the dye). In contrast, laser-induced heating of nanoparticles containing ICG or IR780 produced reactive (1)O2, leading to bleaching of the dye and also decomposition of coencapsulated payload such as the drug doxorubicin. Croc dye was especially useful as a photothermal agent for laser-controlled release of chemically sensitive payload from nanoparticles. Solution state experiments demonstrated repetitive fractional release of water-soluble fluorescent dye from the interior of thermosensitive liposomes. Additional experiments used a focused laser beam to control leakage from immobilized liposomes with very high spatial and temporal precision. The results indicate that fractional photothermal leakage from nanoparticles doped with Croc dye is a promising method for a range of controlled release applications.

Clean Photothermal Heating and Controlled Release From Near Infrared Dye Doped Nanoparticles Without Oxygen Photosensitization

PubMed Central

Guha, Samit; Shaw, Scott K.; Spence, Graeme T.; Roland, Felicia M.; Smith, Bradley D.

2015-01-01

The photothermal heating and release properties of biocompatible organic nanoparticles, doped with a near-infrared croconaine (Croc) dye, were compared with analogous nanoparticles doped with the common near-infrared dyes ICG and IR780. Separate formulations of lipid-polymer-hybrid nanoparticles and liposomes, each containing Croc dye, absorbed strongly at 808 nm and generated clean laser-induced heating (no production of 1O2 and no photobleaching of the dye). In contrast, laser-induced heating of nanoparticles containing ICG or IR780 produced reactive 1O2 leading to bleaching of the dye and also decomposition of co-encapsulated payload such as the drug Doxorubicin. Croc dye was especially useful as a photothermal agent for laser controlled release of chemically sensitive payload from nanoparticles. Solution state experiments demonstrated repetitive fractional release of water soluble fluorescent dye from the interior of thermosensitive liposomes. Additional experiments used a focused laser beam to control leakage from immobilized liposomes with very high spatial and temporal precision. The results indicate that fractional photothermal leakage from nanoparticles doped with Croc dye is a promising method for a range of controlled release applications. PMID:26149326

Christiansen effect in disperse systems with resonant absorption

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zimnyakov, D A; Isaeva, Elmira A; Isaeva, A A

We discuss the results of experimental studies of competition of absorption and scattering of laser radiation propagating in dispersive media with resonant absorption. As media under study, use is made of a suspension of polystyrene particles in solutions of rhodamine 6G in ethylene glycol probed by laser light with a wavelength of 532 nm. It is found that an increase in the dye concentration leads to an increase in optical transmittance of suspensions and an increase in speckle modulation of the forward-scattered radiation. We interpret these features as a manifestation of Christiansen effect in disperse systems with resonance absorption.

Laser lithotripsy of gallstones: alexandrite and rhodamine-6G versus coumarin dye laser: fragmentation and fiber burn-off in vitro

NASA Astrophysics Data System (ADS)

Hochberger, Juergen; Bredt, Marion; Mueller, Gudrun; Hahn, Eckhart G.; Ell, Christian

1993-05-01

In the following study three different pulsed laser lithotripsy systems were compared for the fine fragmentation of identical sets of natural and synthetic gallstones `in vitro.' Using a pulsed coumarin dye laser (504 nm), a pulsed rhodamine 6G dye laser (595 nm), and a pulsed Alexandrite laser (755 nm) a total of 184 concrements of known chemical composition, size, and weight were disintegrated to a fragment size of

Optimal parameters for laser tissue soldering: II. Premixed versus separate dye-solder techniques.

PubMed

McNally, K M; Sorg, B S; Chan, E K; Welch, A J; Dawes, J M; Owen, E R

2000-01-01

Laser tissue soldering by using an indocyanine green (ICG)-doped protein solder applied topically to the tissue surface and denatured with a diode laser was investigated in Part I of this study. The depth of light absorption was predominantly determined by the concentration of the ICG dye added to the solder. This study builds on that work with an in vitro investigation of the effects of limiting the zone of heat generation to the solder-tissue interface to determine whether more stable solder-tissue fusion can be achieved. An alternative laser tissue soldering technique was investigated, which increased light absorption at the vital solder-tissue interface. A thin layer of ICG dye was smeared over the surface to be treated, the protein solder was then placed directly on top of the dye, and the solder was denatured with an 808-nm diode laser. Because laser light at approximately 800 nm is absorbed primarily by the ICG dye, this thin layer of ICG solution restricted the heat source to the space between the solder and the tissue surfaces. A tensile strength analysis was conducted to compare the separate dye-solder technique with conventional techniques of laser tissue soldering for which a premixed dye-solder is applied directly to the tissue surface. The effect of hydration on bond stability of repairs formed by using both techniques was also investigated using tensile strength and scanning electron microscopy analysis. Equivalent results in terms of tensile strength were obtained for the premixed dye-solder technique using protein solders containing 0.25 mg/ml ICG (liquid solder, 220 +/- 35 N/cm(2); solid solder, 602 +/- 32 N/cm(2)) and for the separate dye-solder technique (liquid solder, 228 +/- 41 N/cm(2); solid solder, 578 +/- 29 N/cm(2)). The tensile strength of native bovine thoracic aorta was 596 +/- 31 N/cm(2). Repairs created by using the separate dye-solder technique were more stable during hydration than their premixed dye-solder counterparts. The conventional premixed dye-solder was simpler and approximately twice as fast to apply. The separate dye-solder technique, however, increased the shelf-life of the solder, because the dye was mixed at the time of the experiment, thus conserving its spectral absorbency properties. Two laser-assisted tissue soldering techniques have been evaluated for repairing aorta incisions in vitro. The advantages and disadvantages of each of these techniques are discussed. Copyright 2000 Wiley-Liss, Inc.

Label free biosensor incorporating a replica-molded, vertically emitting distributed feedback laser

NASA Astrophysics Data System (ADS)

Lu, M.; Choi, S. S.; Wagner, C. J.; Eden, J. G.; Cunningham, B. T.

2008-06-01

A label free biosensor based upon a vertically emitting distributed feedback (DFB) laser has been demonstrated. The DFB laser comprises a replica-molded, one-dimensional dielectric grating coated with laser dye-doped polymer as the gain medium. Adsorption of biomolecules onto the laser surface alters the DFB laser emission wavelength, thereby permitting the kinetic adsorption of a protein polymer monolayer or the specific binding of small molecules to be quantified. A bulk sensitivity of 16.6nm per refractive index unit and the detection of a monolayer of the protein polymer poly(Lys, Phe) have been observed with this biosensor. The sensor represents a departure from conventional passive resonant optical sensors from the standpoint that the device actively generates its own narrowband high intensity output without stringent requirements on the coupling alignments, resulting in a simple, robust illumination and detection configuration.

Dye foils with increased durability for passive Q-switching in a 1064 nm laser

NASA Astrophysics Data System (ADS)

Mierczyk, Z.; Kwasny, M.; Czeszko, J.

The results of spectral gel permeation chromatography and differential thermal analysis investigations of structures of dye foils consisting of bis-(4-dimethyl-amino-dithio-benzil)-nickel dye suspended in polymethylmethacrylate matrix, to be used for passive Q-switching in a 1064 nm laser, are reported. Results of experimental measurements and of numerical calculations of thermal and generating properties, and of the endurance of passive foil type Q-switches in the resonator of YAG:Nd(3+) laser are also presented. Optimization of polymerization conditions has enabled the production of dye foils with high thermal and photochemical resistance, which give stable operation of a giant pulsed laser.

Determination of Spatial Distribution of Air Pollution by Dye Laser Measurement of Differential Absorption of Elastic Backscatter

NASA Technical Reports Server (NTRS)

Ahmed, S. A.; Gergely, J. S.

1973-01-01

This paper presents the results of an analytical study of a lidar system which uses tunable organic dye lasers to accurately determine spatial distribution of molecular air pollutants. Also described will be experimental work to date on simultaneous multiwavelength output dye laser sources for this system. Basically the scheme determines the concentration of air pollutants by measuring the differential absorption of an (at least) two wavelength lidar signal elastically backscattered by the atmosphere. Only relative measurements of the backscattered intensity at each of the two wavelengths, one on and one off the resonance absorption of the pollutant in question, are required. The various parameters of the scheme are examined and the component elements required for a system of this type discussed, with emphasis on the dye laser source. Potential advantages of simultaneous multiwavelength outputs are described. The use of correlation spectroscopy in this context is examined. Comparisons are also made for the use of infrared probing wavelengths and sources instead of dye lasers. Estimates of the sensitivity and accuracy of a practical dye laser system of this type, made for specific pollutants, snow it to have inherent advantages over other schemes for determining pollutant spatial distribution.

Oxazine laser dyes

DOEpatents

Hammond, Peter R.; Field, George F.

1992-01-01

New oxazine compounds useful as dye laser media in solution, are superiior to prior art materials. The oxazine dyes useful when pumped by the 578.2 nm copper line to operate in the 700-800 nm range are described by formula I ##STR1##

On-chip tunable optofluidic dye laser

NASA Astrophysics Data System (ADS)

Cai, Zengyan; Shen, Zhenhua; Liu, Haigang; Yue, Huan; Zou, Yun; Chen, Xianfeng

2016-11-01

We demonstrate a chip-scale tunable optofluidic dye laser with Au-coated fibers as microcavity. The chip is fabricated by soft lithography. When the active region is pumped, a relatively low threshold of 6.7 μJ/mm2 is realized with multimode emission due to good confinement of the cavity mirrors, long active region, as well as total reflectivity. It is easy to tune the lasing emission wavelength by changing the solvent of laser dye. In addition, the various intensity ratios of multicolor lasing can be achieved by controlling flow rates of two fluid streams carried with different dye molecules. Furthermore, the convenience in fabrication and directional lasing emission outcoupled by the fiber make the tunable optofluidic dye laser a promising underlying coherent light source in the integrated optofluidic systems.

Phototoxic effect of conjugates of plasmon-resonance nanoparticles with indocyanine green dye on Staphylococcus aureus induced by IR laser radiation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tuchina, E S; Tuchin, Valerii V; Khlebtsov, B N

2011-04-30

The effect of IR laser radiation ({lambda} = 805 - 808 nm) on the bacteria of the strain Staphylococcus aureus 209 P, incubated in indocyanine green solutions, is studied, as well as that of colloid gold nanoshells, nanocages and their conjugates with indocyanine green. It is found that the S. aureus 209 P cells are equally subjected to the IR laser radiation ({lambda} = 805 nm) after preliminary sensitisation with indocyanine green and gold nanoparticles separately and with conjugates of nanoparticles and indocyanine green. The enhancement of photodynamic and photothermal effects by 5 % is observed after 30 min ofmore » laser illumination ({lambda} = 808 nm) of bacteria, treated with conjugates of indocyanine green and nanocages. (optical technologies in biophysics and medicine)« less

Fabrication and characterization of microcavity lasers in rhodamine B doped SU8 using high energy proton beam

NASA Astrophysics Data System (ADS)

Venugopal Rao, S.; Bettiol, A. A.; Vishnubhatla, K. C.; Bhaktha, S. N. B.; Narayana Rao, D.; Watt, F.

2007-03-01

The authors present their results on the characterization of individual dye-doped microcavity polymer lasers fabricated using a high energy proton beam. The lasers were fabricated in rhodamine B doped SU8 resist with a single exposure step followed by chemical processing. The resulting trapezoidal shaped cavities had dimensions of ˜250×250μm2. Physical characterization of these structures was performed using a scanning electron microscope while the optical characterization was carried out by recording the emission subsequent to pumping the lasers with 532nm, 6 nanosecond pulses. The authors observed intense, narrow emission near 624nm with the best emission linewidth full width at half maximum of ˜9nm and a threshold ˜150μJ/mm2.

Hydrogen bonding intermolecular effect on electro-optical response of doped 6PCH nematic liquid crystal with some azo dyes

NASA Astrophysics Data System (ADS)

Kiani, S.; Zakerhamidi, M. S.; Tajalli, H.

2016-05-01

Previous studies on the electro-optical responses of dye-doped liquid crystal have shown that dopant material have a considerable effect on their electro-optical responses. Despite the studies carried out on electro-optical properties of dye-doped liquid crystal, no attention has been paid to study of the interaction and structural effects in this procedure. In this paper, linear dyes and with similar structure were selected as dopants. The only difference in used dyes is the functional groups in their tails. So, doping of these dyes into liquid crystals determines the influence of interaction type on electro-optical behaviours of the doped systems. Therefore, in this work, two aminoazobenzene (;A-dye;: hydrogen bond donor) and dimethyl-aminoazobenzene (;B-dye;) dyes with different compositional percentages in liquid crystal host were used. Electro-optical Kerr behaviour, the pre-transition temperature and third order nonlinear susceptibility were investigated. The obtained results effectively revealed that type of interactions between the dye and liquid crystal is determinative of behavioral difference of doped system, compared to pure liquid crystal. Also, pre-transitional behaviour and thereupon Kerr electro-optical responses were affected by formed interactions into doped systems. In other words, it will be shown that addition of any dopants in liquid crystal, regardless of the nature of interactions, cannot cause appropriate electro-optical responses. In fact, type of dye, nature of interactions between dopant and liquid crystalline host as well as concentration of dye are the key factors in selecting the appropriate liquid crystal and dopant dye.

Optical Sensors Based on Single on Arm Thin Film Waveguide Interferometer

NASA Technical Reports Server (NTRS)

Sarkisov, S. S.; Diggs, D.; Curley, M.; Adamovsky, Grigory (Technical Monitor)

2000-01-01

Single-arm dual-mode optical waveguide interferometer utilizes interference between two modes of different order. Sensing effect results from the change in propagation conditions of the modes caused by the environment. The waveguide is made as an open asymmetric structure containing a dye-doped polymer film onto a quartz substrate. It is more sensitive to the change of environment than its conventional polarimetric analog using orthogonal modes (TE and TM) of the same order. The sensor still preserves the option of operating in polarimetric regime using a variety of mode combinations such as TE(sub 0)/TM(sub 0) (conventional) TE(sub 0)/TM(sub 1), TE(sub 1)/TM(sub 0), or TE(sub 1)/TM(sub 1) but can also work in nonpolarimetric regime using combinations TE(sub 0)/TE(sub 1) or TM(sub 0)/TM(sub 1). Utilization of different mode combinations simultaneously makes the device more versatile. Application of the sensor to gas sensing is based on doping polymer film with an organic indicator dye targeting a particular gaseous reagent. Change of the optical absorption spectrum of the dye caused by the gaseous pollutant results in change of the reactive index of the dye-doped polymer film that can be detected by the sensor. As indicator dyes we utilize Bromocresol Purple doped into polymer poly(methyl) methacrylate that is sensitive to small concentrations of ammonia. The indicator dye demonstrated an irreversible increase in optical absorption near the peak at 350 nm being exposed to 5% ammonia in pure nitrogen at 600 Torr. The dye also showed reversible growth of the absorption peak near 600 nm after exposure to a vapor of standard medical ammonia spirit (65% alcohol). We have built a breadboard prototype of the sensor with He-Ne laser as a light source and with a single mode fiber input and a multimode fiber output. The prototype showed a sensitivity to temperature change of the order of 2 C per 2pi phase shift. The sensitivity of the sensor to the presence of dTy ammonia is not less than 300 ppm per 2pi phase shift. The proposed sensor can be used as a robust stand-alone instrument for continuous environment pollution monitoring.

PicoGreen dye as an active medium for plastic lasers

NASA Astrophysics Data System (ADS)

Pradeep, C.; Vallabhan, C. P. G.; Radhakrishnan, P.; Nampoori, V. P. N.

2015-08-01

Deoxyribonucleic acid lipid complex thin films are used as a host material for laser dyes. We tested PicoGreen dye, which is commonly used for the quantification of single and double stranded DNA, for its applicability as lasing medium. PicoGreen dye exhibits enhanced fluorescence on intercalation with DNA. This enormous fluorescence emission is amplified in a planar microcavity to achieve yellow lasing. Here the role of DNA is not only a host medium, but also as a fluorescence dequencher. With the obtained results we have ample reasons to propose PicoGreen dye as a lasing medium, which can lead to the development of DNA based bio-lasers.

Inelastic hyperspectral lidar for aquatic ecosystems monitoring and landscape plant scanning test

NASA Astrophysics Data System (ADS)

Zhao, Guangyu; Malmqvist, Elin; Rydhmer, Klas; Strand, Alfred; Bianco, Giuseppe; Hansson, Lars-Anders; Svanberg, Sune; Brydegaard, Mikkel

2018-04-01

We have developed an aquatic inelastic hyperspectral lidar with unrestricted focal-depth and enough sensitivity and spatio-temporal resolution to detect and resolve position and behavior of individual sub-millimeter aquatic organisms. We demonstrate ranging with monitoring of elastic echoes, water Raman signals and fluorescence from chlorophyllbearing phytoplankton and dye tagged organisms. The system is based on a blue CW diode laser and a Scheimpflug optical arrangement.

Thermal cycling and the optical and electrical characterization of self-assembled multilayer nile blue A-gold thin films.

PubMed

Geist, Brian; Spillman, William B; Claus, Richard O

2005-10-20

Some laser applications produce high power densities that can be dangerous to equipment and operators. We have fabricated thin-film coatings by using molecular electrostatic self-assembly to create a spectrally selective absorbing coating that is able to withstand thermal fluctuations from -20 degrees C to 120 degrees C. We made the thin-film coatings by alternating deposition of an organic dye and gold colloidal nanoparticles onto glass substrates. Nile Blue A perchlorate, with a maximum absorbance slightly above 632 nm, was chosen as the organic dye. Strong coupling between the dye molecules and the gold nanoparticles provides a redshift that increases as the film's thickness is increased. The incorporation of the gold colloidal nanoparticles also decreases the resistivity of the film. The resistivity of the film was measured with a four-point probe and found to be approximately 10 omega/cm for the two samples measured. Atomic-force microscopy was used to show that film thickness increased 2.4 nm per bilayer. The optical properties of the film were measured at the end of every 5 thermal cycles from -20 degrees C to 120 degrees C, and negligible degradation was observed after 30 cycles.

Active thermal fine laser tuning in a broad spectral range and optical properties of cholesteric liquid crystal.

PubMed

Jeong, Mi-Yun; Kwak, Keumcheol

2016-11-20

In this study, we achieved active fine laser tuning in a broad spectral range with dye-doped cholesteric liquid crystal wedge-type cells through temperature control. The spatial pitch gradient of each position of the wedge cell at room temperature was almost maintained after developing a temperature gradient. To achieve the maximum tuning range, the chiral dopant concentration, thickness, thickness gradient, and temperature gradient on the wedge cell should be matched properly. In order to understand the laser tuning mechanism for temperature change, we studied the temperature dependence of optical properties of the photonic bandgap of cholesteric liquid crystals. In our cholesteric liquid crystal samples, when temperature was increased, photonic bandgaps were shifted toward blue, while the width of the photonic bandgap was decreased, regardless of whether the helicity was left-handed or right-handed. This is mainly due to the combination of decreased refractive indices, higher molecular anisotropy of chiral molecules, and increased chiral molecular solubility. We envisage that this kind of study will prove useful in the development of practical active tunable CLC laser devices.

Pulsed laser synthesis in liquid of efficient visible-light-active ZnO/rGO nanocomposites for improved photo-catalytic activity

NASA Astrophysics Data System (ADS)

Moqbel, Redhwan A.; Gondal, Mohammed A.; Qahtan, Talal F.; Dastageer, Mohamed A.

2018-03-01

In this work the synthesis of visible light active zinc oxide/reduced graphene oxide (ZnO/rGO) nanocomposite by laser induced fragmentation of particulates in liquid, its morphological/optical characterizations, and its application in the process of photo-catalytic degradation of toxic Rhodamine B (RhB) dye under visible radiation were studied. It is observed from the optical and morphological characterization that the anchoring of ZnO on the rGO sheets in ZnO/rGO nanocomposite considerably reduced the aggregation of ZnO (increased surface area), reduced the recombination of photo-induced charge carriers, promoted more adsorption of reactants on the catalytic surface and also enhanced and extended the light absorption in the visible spectral region. With all these improved characteristics of ZnO/rGO nanocomposite, it was found that this material as a photo-catalyst yielded an RhB degradation efficiency of 86%, as compared to the 40% degradation with pure ZnO NPs under the same experimental conditions. In the ZnO/rGO nanocomposite, rGO functions as an electron acceptor to promote charge separation, an aggregation inhibitor to enhance the active surface area, a co-catalyst, a good dye adsorber and also as a supporting matrix for ZnO.

Intravital multiphoton photoconversion with a cell membrane dye.

PubMed

Turcotte, Raphaël; Wu, Juwell W; Lin, Charles P

2017-02-01

Photoconversion, an irreversible shift in a fluorophore emission spectrum after light exposure, is a powerful tool for marking cellular and subcellular compartments and tracking their dynamics in vivo. This paper reports on the photoconversion properties of Di-8-ANEPPS, a commercially available membrane dye. When illuminated with near-infrared femtosecond laser pulses, Di-8-ANEPPS undergoes multiphoton photoconversion as indicated by the supralinear dependence of the conversion rate ρ pc on the incident power (ρpc∝Iexc2.27), and by the ability to photoconvert a thin optical section in a three-dimensional matrix. The characteristic emission spectrum changed from red to blue, and ratiometric analysis on single cells in vitro revealed a 65-fold increase in the blue to red wavelength ratio after photoconversion. The spectral shift is preserved in vivo for hours, making Di-8-ANEPPS a useful dye for intravital cell marking and tracking applications. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Picosecond pulse measurements using the active laser medium

NASA Technical Reports Server (NTRS)

Bernardin, James P.; Lawandy, N. M.

1990-01-01

A simple method for measuring the pulse lengths of synchronously pumped dye lasers which does not require the use of an external nonlinear medium, such as a doubling crystal or two-photon fluorescence cell, to autocorrelate the pulses is discussed. The technique involves feeding the laser pulses back into the dye jet, thus correlating the output pulses with the intracavity pulses to obtain pulse length signatures in the resulting time-averaged laser power. Experimental measurements were performed using a rhodamine 6G dye laser pumped by a mode-locked frequency-doubled Nd:YAG laser. The results agree well with numerical computations, and the method proves effective in determining lengths of picosecond laser pulses.

On-Chip Photothermal Analyte Detection Using Integrated Luminescent Temperature Sensors.

PubMed

Pfeiffer, Simon A; Nagl, Stefan

2017-09-05

Optical absorbance detection based on attenuated light transmission is limited in sensitivity due to short path lengths in microfluidic and other miniaturized platforms. An alternative is detection using the photothermal effect. Herein we introduce a new kind of photothermal absorbance measurement using integrated luminescent temperature sensor spots inside microfluidic channels. The temperature sensors were photopolymerized inside the channels from NOA 81 UV-curable thiolene prepolymer doped with a tris(1,10-phenanthroline)ruthenium(II) temperature probe. The polymerized sensing structures were as small as 26 ± 3 μm in diameter and displayed a temperature resolution of better than 0.3 K between 20 and 50 °C. The absorbance from 532 nm laser excitation of the food dye Amaranth as a model analyte was quantified using these spots, and the influence of the flow rate, laser power, and concentration was investigated. Calibration yielded a linear relationship between analyte concentration and the temperature signal in the channels. The limit of detection for the azo-dye Amaranth (E123) in this setup was 13 μM. A minimal detectable absorbance of 3.2 × 10 -3 AU was obtained using an optical path length of 125 μm in this initial study. A microreactor with integrated temperature sensors was then employed for an absorbance-based miniaturized nitrite analysis, yielding a detection limit of 26 μM at a total assay time of only 75 s. This technique is very promising for sensitive, and potentially spatially resolved, optical absorbance detection on the micro- and nanoscale.

Dye system for dye laser applications

DOEpatents

Hammond, Peter R.

1991-01-01

A dye of the DCM family, [2-methyl-6-[2-(1,2,3,4-tetrahydro-1-methyl-6-quinolinyl)ethenyl]-4H-pyran -4-ylidene]-propanedinitrile, dissolved in 2-phenoxyethanol, is non-mutagenic, stable and efficient, particularly in a pumped continuous wave laser system.

Joint study of lipopolysaccharide suspensions with thermal lensing and optoacoustic methods

NASA Astrophysics Data System (ADS)

Orlova, Nataliya V.; Brusnichkin, Anton V.; Proskurnin, Mikhail A.; Fokin, Andrey V.; Ovchinnikov, Oleg B.; Egerev, Sergey V.

2004-07-01

Pyrogens being introduced intravenously increase body temperature that leads to hazardous consequences and even to lethal outcome. One of the widespread pyrogen systems is presented by suspensions composed of bacterial endotoxins (or lypopolysaccharides, LPS). The aim of the work is to compare experimentally two methods for the determination of LPS at the submicrogram level and below. Both methods suppose that the LPS suspension is irradiated by a laser pulse. The thermal lens (TL) method (microsecond to millisecond irradiation cycle) detects LPS by a direct pick-up of the transient thermal field. The optoacoustic (OA) method (nanosecond laser pulses) has a potential to use non-thermal constitutents of the LPS response and to provide some selectivity of LPS detection with respect to optically uniform contaminants in the sample. In experiments, the selectivity was enhanced by means of analytical reagents, methylene blue and Stains All dyes. It was shown that both methods are mutually complementary. Then, their detectability potential increases and reaches 10 ppb if there occur ion pairs of LPS and cationic dye.

Lasing in silicon–organic hybrid waveguides

PubMed Central

Korn, Dietmar; Lauermann, Matthias; Koeber, Sebastian; Appel, Patrick; Alloatti, Luca; Palmer, Robert; Dumon, Pieter; Freude, Wolfgang; Leuthold, Juerg; Koos, Christian

2016-01-01

Silicon photonics enables large-scale photonic–electronic integration by leveraging highly developed fabrication processes from the microelectronics industry. However, while a rich portfolio of devices has already been demonstrated on the silicon platform, on-chip light sources still remain a key challenge since the indirect bandgap of the material inhibits efficient photon emission and thus impedes lasing. Here we demonstrate a class of infrared lasers that can be fabricated on the silicon-on-insulator (SOI) integration platform. The lasers are based on the silicon–organic hybrid (SOH) integration concept and combine nanophotonic SOI waveguides with dye-doped organic cladding materials that provide optical gain. We demonstrate pulsed room-temperature lasing with on-chip peak output powers of up to 1.1 W at a wavelength of 1,310 nm. The SOH approach enables efficient mass-production of silicon photonic light sources emitting in the near infrared and offers the possibility of tuning the emission wavelength over a wide range by proper choice of dye materials and resonator geometry. PMID:26949229

Dye Foils With Increased Durability For Passive Q-Switching In A 1064 Nm Laser.

NASA Astrophysics Data System (ADS)

Mierczyk, Z.; Kwasny, M.; Czeszko, J.

1987-10-01

The results of spectral (IR, UV-VIS, H NMR) , gel permeation chromatography and differential thermal analysis investigations of structures of dye foils consisting of bis-(4-dimethyl-amino-dithio-benzil)-nickel dye suspended in polymethylmethacrylate matrix, to be used for passive Q-switching in a 1064 nm laser, are reported. Results of experimental measurements and of numerical calculations of thermal and generating properties, and of the endurance of passive foil type Q-switches in the resona-tor of YAG:Nd3+ laser are also presented. Optimization of polymerization conditions has enabled the production of dye foils with high thermal and photochemical resistance, which give stable operation of a giant pulsed laser.

Co-extruded mechanically tunable multilayer elastomer laser

NASA Astrophysics Data System (ADS)

Crescimanno, Michael; Mao, Guilin; Andrews, James; Singer, Kenneth; Baer, Eric; Hiltner, Anne; Song, Hyunmin; Shakya, Bijayandra

2011-04-01

We have fabricated and studied mechanically tunable elastomer dye lasers constructed in large area sheets by a single-step layer-multiplying co-extrusion process. The laser films consist of a central dye-doped (Rhodamine-6G) elastomer layer between two 128-layer distributed Bragg reflector (DBR) films comprised of alternating elastomer layers with different refractive indices. The central gain layer is formed by folding the coextruded DBR film to enclose a dye-doped skin layer. By mechanically stretching the elastomer laser film from 0% to 19%, a tunable miniature laser source was obtained with ˜50 nm continuous tunability from red to green.

An All-Solid-State High Repetiton Rate Titanium:Sapphire Laser System For Resonance Ionization Laser Ion Sources

NASA Astrophysics Data System (ADS)

Mattolat, C.; Rothe, S.; Schwellnus, F.; Gottwald, T.; Raeder, S.; Wendt, K.

2009-03-01

On-line production facilities for radioactive isotopes nowadays heavily rely on resonance ionization laser ion sources due to their demonstrated unsurpassed efficiency and elemental selectivity. Powerful high repetition rate tunable pulsed dye or Ti:sapphire lasers can be used for this purpose. To counteract limitations of short pulse pump lasers, as needed for dye laser pumping, i.e. copper vapor lasers, which include high maintenance and nevertheless often only imperfect reliability, an all-solid-state Nd:YAG pumped Ti:sapphire laser system has been constructed. This could complement or even replace dye laser systems, eliminating their disadvantages but on the other hand introduce shortcomings on the side of the available wavelength range. Pros and cons of these developments will be discussed.

Multi-wavelength emission through self-induced second-order wave-mixing processes from a Nd3+ doped crystalline powder random laser

NASA Astrophysics Data System (ADS)

Moura, André L.; Jerez, Vladimir; Maia, Lauro J. Q.; Gomes, Anderson S. L.; de Araújo, Cid B.

2015-09-01

Random lasers (RLs) based on neodymium ions (Nd3+) doped crystalline powders rely on multiple light scattering to sustain laser oscillation. Although Stokes and anti-Stokes Nd3+ RLs have been demonstrated, the optical gain obtained up to now was possibly not large enough to produce self-frequency conversion. Here we demonstrate self-frequency upconversion from Nd3+ doped YAl3(BO3)4 monocrystals excited at 806 nm, in resonance with the Nd3+ transition 4I9/2 → 4F5/2. Besides the observation of the RL emission at 1062 nm, self-converted second-harmonic at 531 nm, and self-sum-frequency generated emission at 459 nm due to the RL and the excitation laser at 806 nm, are reported. Additionally, second-harmonic of the excitation laser at 403 nm was generated. These results exemplify the first multi-wavelength source of radiation owing to nonlinear optical effect in a Nd3+ doped crystalline powder RL. Contrary to the RLs based on dyes, this multi-wavelength light source can be used in photonic devices due to the large durability of the gain medium.

Off-resonant third-order optical nonlinearities of squarylium and croconium dyes

NASA Astrophysics Data System (ADS)

Li, Zhongyu; Xu, Song; Niu, Lihong; Zhang, Zhi; Chen, Zihui; Zhang, Fushi

2008-01-01

The magnitude and dynamic response of the third-order optical nonlinearities of squarylium and croconium dyes in methanol solution were measured by femtosecond degenerate four-wave mixing (DFWM) technique at 800 nm. Ultrafast nonlinear optical responses have been observed, and the magnitude of the second-order hyperpolarizabilities was evaluated to be 5.80 × 10 -31 esu for the squarylium dye and 8.69 × 10 -31 esu for the croconium dye, respectively. The large optical nonlinearities of the dyes can be attributed to their rigid and intramolecular charge transfer structure, and the instantaneous NLO responses of dyes are shorter than the experimental time resolution (50 fs), which is mainly contributed from the electron delocalization. The fast nonlinear response and large third-order optical nonlinearities show that the studied squarylium and croconium dyes might a kind of promising materials for the applications in all-optical switching and modulator.

Violet laser diodes as light sources for cytometry.

PubMed

Shapiro, H M; Perlmutter, N G

2001-06-01

Violet laser diodes have recently become commercially available. These devices emit 5-25 mW in the range of 395-415 nm, and are available in systems that incorporate the diodes with collimating optics and regulated power supplies in housing incorporating thermoelectric coolers, which are necessary to maintain stable output. Such systems now cost several thousand dollars, but are expected to drop substantially in price. Materials and Methods A 4-mW, 397-nm violet diode system was used in a laboratory-built flow cytometer to excite fluorescence of DAPI and Hoechst dyes in permeabilized and intact cells. Forward and orthogonal light scattering were also measured. DNA content histograms with good precision (G(0)/G(1) coefficient of variation 1.7%) were obtained with DAPI staining; precision was lower using Hoechst 33342. Hoechst 34580, with an excitation maximum nearer 400 nm, yielded the highest fluorescence intensity, but appeared to decompose after a short time in solution. Scatter signals exhibited relatively broad distributions. Violet laser diodes are relatively inexpensive, compact, efficient, and quiet light sources for DNA fluorescence measurement using DAPI and Hoechst dyes; they can also excite several other fluorescent probes. Copyright 2001 Wiley-Liss, Inc.

Simulation studies of vapor bubble generation by short-pulse lasers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Amendt, P.; London, R.A.; Strauss, M.

1997-10-26

Formation of vapor bubbles is characteristic of many applications of short-pulse lasers in medicine. An understanding of the dynamics of vapor bubble generation is useful for developing and optimizing laser-based medical therapies. To this end, experiments in vapor bubble generation with laser light deposited in an aqueous dye solution near a fiber-optic tip have been performed. Numerical hydrodynamic simulations have been developed to understand and extrapolate results from these experiments. Comparison of two-dimensional simulations with the experiment shows excellent agreement in tracking the bubble evolution. Another regime of vapor bubble generation is short-pulse laser interactions with melanosomes. Strong shock generationmore » and vapor bubble generation are common physical features of this interaction. A novel effect of discrete absorption by melanin granules within a melanosome is studied as a possible role in previously reported high Mach number shocks.« less

Feedback mechanism for smart nozzles and nebulizers

DOEpatents

Montaser, Akbar [Potomac, MD; Jorabchi, Kaveh [Arlington, VA; Kahen, Kaveh [Kleinburg, CA

2009-01-27

Nozzles and nebulizers able to produce aerosol with optimum and reproducible quality based on feedback information obtained using laser imaging techniques. Two laser-based imaging techniques based on particle image velocimetry (PTV) and optical patternation map and contrast size and velocity distributions for indirect and direct pneumatic nebulizations in plasma spectrometry. Two pulses from thin laser sheet with known time difference illuminate droplets flow field. Charge coupled device (CCL)) captures scattering of laser light from droplets, providing two instantaneous particle images. Pointwise cross-correlation of corresponding images yields two-dimensional velocity map of aerosol velocity field. For droplet size distribution studies, solution is doped with fluorescent dye and both laser induced florescence (LIF) and Mie scattering images are captured simultaneously by two CCDs with the same field of view. Ratio of LIF/Mie images provides relative droplet size information, then scaled by point calibration method via phase Doppler particle analyzer.

Skin closure with dye-enhanced laser welding and fibrinogen.

PubMed

Wider, T M; Libutti, S K; Greenwald, D P; Oz, M C; Yager, J S; Treat, M R; Hugo, N E

1991-12-01

The topical application of wavelength-specific dye and fibrinogen has been used to enhance laser closure of vascular anastomoses. We compared the closure of skin incisions by two different dye-enhanced, fibrinogen-based laser welding systems [argon laser (power density 4.78 W/cm2) with fluorescein isothiocyanate dye (n = 32) and diode laser (power density 9.55 W/cm2) with indocyanine green dye (n = 32)] with closure by interrupted 5-0 nylon suture (n = 64) and examined tensile strength, hydroxyproline production, histology, and cosmesis. Two 3-cm full-thickness incisions were made on the shaved backs of 64 rats. One incision was closed with suture, whereas the other, after treatment with the appropriate dye, was welded with either argon- or diode-lasered fibrinogen. At postoperative days 5, 10, 15, and 28, the closure sites were harvested and sectioned for analysis. Initially, wounds closed with argon-lasered fibrinogen showed less inflammatory response, greater collagen production (34.61 +/- 0.74 mg/gm), and greater mean peak stress at rupture (64.85 lbs/in2) than those closed with suture (16.42 +/- 3.20 mg/gm, 26.68 lbs/in2) (p less than 0.05). By 15 days, both argon and diode laser closures are superior in strength and collagen production to suture closure (p less than 0.05). At 28 days, diode laser closures (1315.60 lbs/in2) are stronger than suture closures (998.09 lbs/in2), whereas both are stronger than argon laser closures (813.16 lbs/in2) (p less than 0.05). Cosmetically, argon-welded wounds consistently appeared finer and lacked cross-hatched suture scars.(ABSTRACT TRUNCATED AT 250 WORDS)

Optical fiber-based setup for in vivo measurement of the delayed fluorescence lifetime of oxygen sensors

NASA Astrophysics Data System (ADS)

Piffaretti, Filippo M.; Santhakumar, Kanappan; Forte, Eddy; van den Bergh, Hubert E.; Wagnières, Georges A.

2011-03-01

A new optical-fiber-based spectrofluorometer for in vivo or in vitro detection of delayed fluorescence is presented and characterized. This compact setup is designed so that it can be readily adapted for future clinical use. Optical excitation is done with a nitrogen laser-pumped, tunable dye laser, emitting in the UV-vis part of the spectrum. Excitation and luminescence signals are carried to and from the biological tissues under investigation, located out of the setup enclosure, by a single optical fiber. These measurements, as well as measurements performed without a fiber on in vitro samples in a thermostable quartz cell, in a controlled-atmosphere enclosure, are possible due to the efficient collection of the laser-induced luminescence light which is collected and focused on the detector with a high aperture parabolic mirror. The detection is based on a gated photomultiplier which allows for time-resolved measurements of the delayed fluorescence intensity. Thus, relevant luminescence lifetimes, typically in the sub-microsecond-to-millisecond range, can be measured with near total rejection of the sample's prompt fluorescence. The instrument spectral and temporal resolution, as well as its sensitivity, is characterized and measurement examples are presented. The primary application foreseen for this setup is the monitoring and adjustment of the light dose delivered during photodynamic therapy.

In vitro photoacoustic measurement of hemoglobin oxygen saturation using a single pulsed broadband supercontinuum laser source.

PubMed

Lee, Changho; Jeon, Mansik; Jeon, Min Yong; Kim, Jeehyun; Kim, Chulhong

2014-06-20

We have utilized a single pulsed broadband supercontinuum laser source to photoacoustically sense total hemoglobin concentration (HbT) and oxygen saturation of hemoglobin (SO2) in bloods in vitro. Unlike existing expensive and bulky laser systems typically used for functional photoacoustic imaging (PAI), our laser system is relatively cost-effective and compact. Instead of using two single wavelengths, two wavelength bands were applied to distinguish the concentrations of two different chromophores in the mixture. In addition, we have successfully extracted the total dye concentration and the ratio of the red dye concentration to the total dye concentration in mixed red and blue dye solutions in phantoms. The results indicate that PAI with a cheap and compact fiber based laser source can potentially provide HbT and SO2 in live animals in vivo.

Numerical investigation of vessel heating using a copper vapor laser and a pulsed dye laser in treating vascular skin lesions

NASA Astrophysics Data System (ADS)

Pushkareva, A. E.; Ponomarev, I. V.; Isaev, A. A.; Klyuchareva, S. V.

2018-02-01

A computer simulation technique was employed to study the selective heating of a tissue vessel using emission from a pulsed copper vapor laser and a pulsed dye laser. The depth and size of vessels that could be selectively and safely removed were determined for the lasers under examination.

Directional emission from dye-functionalized plasmonic DNA superlattice microcavities

PubMed Central

Park, Daniel J.; Ku, Jessie C.; Sun, Lin; Lethiec, Clotilde M.; Stern, Nathaniel P.; Schatz, George C.; Mirkin, Chad A.

2017-01-01

Three-dimensional plasmonic superlattice microcavities, made from programmable atom equivalents comprising gold nanoparticles functionalized with DNA, are used as a testbed to study directional light emission. DNA-guided nanoparticle colloidal crystallization allows for the formation of micrometer-scale single-crystal body-centered cubic gold nanoparticle superlattices, with dye molecules coupled to the DNA strands that link the particles together, in the form of a rhombic dodecahedron. Encapsulation in silica allows one to create robust architectures with the plasmonically active particles and dye molecules fixed in space. At the micrometer scale, the anisotropic rhombic dodecahedron crystal habit couples with photonic modes to give directional light emission. At the nanoscale, the interaction between the dye dipoles and surface plasmons can be finely tuned by coupling the dye molecules to specific sites of the DNA particle-linker strands, thereby modulating dye–nanoparticle distance (three different positions are studied). The ability to control dye position with subnanometer precision allows one to systematically tune plasmon–excition interaction strength and decay lifetime, the results of which have been supported by electrodynamics calculations that span length scales from nanometers to micrometers. The unique ability to control surface plasmon/exciton interactions within such superlattice microcavities will catalyze studies involving quantum optics, plasmon laser physics, strong coupling, and nonlinear phenomena. PMID:28053232

Time-Delayed Two-Step Selective Laser Photodamage of Dye-Biomolecule Complexes

NASA Astrophysics Data System (ADS)

Andreoni, A.; Cubeddu, R.; de Silvestri, S.; Laporta, P.; Svelto, O.

1980-08-01

A scheme is proposed for laser-selective photodamage of biological molecules, based on time-delayed two-step photoionization of a dye molecule bound to the biomolecule. The validity of the scheme is experimentally demonstrated in the case of the dye Proflavine, bound to synthetic polynucleotides.

Comparison of pulsed dye laser versus combined pulsed dye laser and Nd:YAG laser in the treatment of inflammatory acne vulgaris.

PubMed

Salah El Din, Manal Mohamed; Samy, Nevien Ahmed; Salem, Amira Eid

2017-06-01

Both pulsed dye laser and combined 585/1064-nm (sequential dual-wavelength PDL and Nd:YAG) laser improves inflammatory skin disorders including acne vulgaris. To compare the efficacy of 585-nm pulsed dye laser versus sequential dual-wavelength PDL and Nd:YAG in treatment of acne vulgaris. Thirty patients with acne vulgaris were treated by PDL alone on half of the face while contra lateral half was treated by combined 585/1064 nm laser. The study showed that inflammatory acne lesions count was significantly reduced by 82.5% (p 0.0001) on PDL sides and by 83.5% (p 0.00001) on combined 585/1064-nm side after 8 weeks, while reduction of non-inflammatory acne lesions was observed at 8 weeks by 58.4% and 71.5% respectively. However, difference between the two modalities was not statistically significant. PDL and combined PDL/Nd:YAG laser treatment were found to be an effective, safe and well-tolerated treatment option for inflammatory and non-inflammatory acne vulgaris.

Direct Analysis of Textile Fabrics and Dyes Using IR Matrix-Assisted Laser Desorption Electrospray Ionization (MALDESI) Mass Spectrometry

PubMed Central

Cochran, Kristin H.; Barry, Jeremy A.; Muddiman, David C.; Hinks, David

2012-01-01

The forensic analysis of textile fibers uses a variety of techniques from microscopy to spectroscopy. One such technique that is often used to identify the dye(s) within the fiber is mass spectrometry (MS). In the traditional MS method, the dye must be extracted from the fabric and the dye components are separated by chromatography prior to mass spectrometric analysis. Direct analysis of the dye from the fabric allows the omission of the lengthy sample preparation involved in extraction, thereby significantly reducing the overall analysis time. Herein, a direct analysis of dyed textile fabric was performed using the infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) source for MS. In MALDESI, an IR laser with wavelength tuned to 2.94 μm is used to desorb the dye from the fabric sample with the aid of water as the matrix. The desorbed dye molecules are then post-ionized by electrospray ionization (ESI). A variety of dye classes were analyzed from various fabrics with little to no sample preparation allowing for the identification of the dye mass and in some cases the fiber polymer. Those dyes that were not detected using MALDESI were also not observed by direct infusion ESI of the dye standard. PMID:23237031

LASER APPLICATIONS AND OTHER TOPICS IN QUANTUM ELECTRONICS: Study of relaxation times of polymethine dyes used for passive mode locking of solid-state lasers emitting between 750 and 850 nm

NASA Astrophysics Data System (ADS)

Grigonis, R.; Derevyanko, Nadezhda A.; Ishchenko, Aleksandr A.; Sirutkaitis, V. A.

2001-11-01

The relaxation times τ of the bleached states of polymethine dyes absorbing light in the 750 — 850-nm are determined by the direct pump — probe method. The effect of the dye structure and the solvent type on the relaxation time is discussed. The role of different intra- and intermolecular interactions in the relaxation of excited electronic states of the dyes is analysed. Polymethine dyes are found (with τ=11 — 75 ps) that are promising for passive mode locking in Cr3+:LiCaAlF6, Cr3+:KZnF3, and Cr3+:LiSrAlF6 crystal lasers.

Photodynamic dye adsorption and release performance of natural zeolite

NASA Astrophysics Data System (ADS)

Hovhannisyan, Vladimir; Dong, Chen-Yuan; Chen, Shean-Jen

2017-03-01

Clinoptilolite type of zeolite (CZ) is a promising material for biomedicine and pharmaceutics due to its non-toxicity, thermal stability, expanded surface area, and exceptional ability to adsorb various atoms and organic molecules into micropores. Using multiphoton microscopy, we demonstrated that individual CZ particles produce two-photon excited luminescence and second harmonic generation signal at femtosecond laser excitation, and adsorb photo-dynamically active dyes such as hypericin and methylene blue. Furthermore, the release of hypericin from CZ pores in the presence of biomolecules is shown, and CZ can be considered as an effective material for drug delivery and controlled release in biological systems. The results may open new perspectives in application of CZ in biomedical imaging, and introducing of the optical approaches into the clinical environment.

Photodynamic dye adsorption and release performance of natural zeolite.

PubMed

Hovhannisyan, Vladimir; Dong, Chen-Yuan; Chen, Shean-Jen

2017-03-31

Clinoptilolite type of zeolite (CZ) is a promising material for biomedicine and pharmaceutics due to its non-toxicity, thermal stability, expanded surface area, and exceptional ability to adsorb various atoms and organic molecules into micropores. Using multiphoton microscopy, we demonstrated that individual CZ particles produce two-photon excited luminescence and second harmonic generation signal at femtosecond laser excitation, and adsorb photo-dynamically active dyes such as hypericin and methylene blue. Furthermore, the release of hypericin from CZ pores in the presence of biomolecules is shown, and CZ can be considered as an effective material for drug delivery and controlled release in biological systems. The results may open new perspectives in application of CZ in biomedical imaging, and introducing of the optical approaches into the clinical environment.

Optical gain characteristics of C 460 and C 450.

PubMed

Sharma, Vijay K; Sahare, P D; Pandey, A; Mohan, D

2003-03-15

Dye concentration dependent gain spectra for Coumarin 460 (C 460) and Coumarin 450 (C 450) in ethanol have been studied using Amplified Spontaneous Emission (ASE) technique under Nitrogen laser (337.1 nm) excitation in the concentration range 10(-2)-10(-5) m/l. The dependence of lasing wavelength and peak gain on concentration have been understood in terms of variation of fluorescence lifetime, which is due to photo-physical processes such as radiation trapping and concentration-quenching. Pump intensity dependence of efficiency is also explained in terms of fluorescence lifetime. A comparison of the stability of the two dyes has also been made on the basis of the functional groups at different positions of the basic coumarin. Copyright 2002 Elsevier Science B.V.

In-vivo fluorescence detection of breast cancer growth factor receptors by fiber-optic probe

NASA Astrophysics Data System (ADS)

Bustamante, Gilbert; Wang, Bingzhi; DeLuna, Frank; Sun, LuZhe; Ye, Jing Yong

2018-02-01

Breast cancer treatment options often include medications that target the overexpression of growth factor receptors, such as the proto-oncogene human epidermal growth factor receptor 2 (HER2/neu) and epidermal growth factor receptor (EGFR) to suppress the abnormal growth of cancerous cells and induce cancer regression. Although effective, certain treatments are toxic to vital organs, and demand assurance that the pursued receptor is present at the tumor before administration of the drug. This requires diagnostic tools to provide tumor molecular signatures, as well as locational information. In this study, we utilized a fiber-optic probe to characterize in vivo HER2 and EGFR overexpressed tumors through the fluorescence of targeted dyes. HER2 and EGFR antibodies were conjugated with ICG-Sulfo-OSu and Alexa Fluor 680, respectively, to tag BT474 (HER2+) and MDA-MB-468 (EGFR+) tumors. The fiber was inserted into the samples via a 30-gauge needle. Different wavelengths of a supercontinuum laser were selected to couple into the fiber and excite the corresponding fluorophores in the samples. The fluorescence from the dyes was collected through the same fiber and quantified by a time-correlated single photon counter. Fluorescence at different antibody-dye concentrations was measured for calibration. Mice with subcutaneous HER2+ and/or EGFR+ tumors received intravenous injections of the conjugates and were later probed at the tumor sites. The measured fluorescence was used to distinguish between tumor types and to calculate the concentration of the antibody-dye conjugates, which were detectable at levels as low as 40 nM. The fiber-optic probe presents a minimally invasive instrument to characterize the molecular signatures of breast cancer in vivo.

Wide-band tunable photonic bandgap device and laser in dye-doped liquid crystal refilled cholesteric liquid crystal polymer template system

NASA Astrophysics Data System (ADS)

Lin, Jia-De; Lin, Hong-Lin; Lin, Hsin-Yu; Wei, Guan-Jhong; Lee, Chia-Rong

2017-02-01

The scientists in the field of liquid crystal (LC) have paid significant attention in the exploration of novel cholesteric LC (CLC) polymer template (simply called template) in recent years. The self-assembling nanostructural template with chirality can effectively overcome the limitation in the optical features of traditional CLCs, such as enhancement of reflectivity over 50%, multiple photonic bandgaps (PBGs), and changeable optical characteristics by flexibly replacing the refilling LC materials, and so on. This work fabricates two gradient-pitched CLC templates with two opposite handednesses, which are then merged as a spatially tunable and highly reflective CLC template sample. This sample can simultaneously reflect right- and left-circularly polarized lights and the tunable spectral range includes the entire visible region. By increasing the temperature of the template sample exceeding the clearing point of the refilling LC, the light scattering significantly decreases and the reflectance effectively increase to exceed 50% in the entire visible region. This device has a maximum reflectance over 85% and a wide-band spatial tunability in PBG between 400 nm and 800 nm which covers the entire visible region. Not only the sample can be employed as a wide-band spatially tunable filter, but also the system doping with two suitable laser dyes which emitted fluorescence can cover entire visible region can develop a low-threshold, mirror-less laser with a spatial tunability at spectral regions including blue to red region (from 484 nm to 634 nm) and simultaneous lasing emission of left- and right-circular polarizations.

Improving the Performance of Gold-Nanoparticle-Doped Solid-State Dye Laser Using Thermal Conversion Effect

NASA Astrophysics Data System (ADS)

An, N. T. M.; Lien, N. T. H.; Hoang, N. D.; Hoa, D. Q.

2018-04-01

Energy transfer between spherical gold nanoparticles with size of more than 15 nm and molecules of organic dye 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4 H-pyran (DCM) has been studied. Such radiative energy transfer led to high local temperature, giving rise to a bleaching effect that resulted in rapid degradation of the laser medium. Gold nanoparticles were dispersed at concentrations from 5 × 109 particles/mL to 5 × 1010 particles/mL in DCM polymethylmethacrylate polymer using a radical polymerization process with 2,2'-azobis(isobutyronitrile) (AIBN) as initiator. Using the fast thermoelectric cooling method, the laser medium stability was significantly improved. The output stability of a distributed feedback dye laser pumped by second-harmonic generation from a neodymium-doped yttrium aluminum garnet (Nd:YAG) laser was investigated. Moreover, bidirectional energy transfer between gold nanoparticles and dye molecules was observed.

Hemangiomas

MedlinePlus

... grow. The flash-lamp pulse dye, pump dye, diode, and sclero-laser are the primary lasers used ... go through several trials of dosing-tapering. The current protocol is to put them on the initial ...

Laser desorption/ionization mass spectrometry of dye-sensitized solar cells: identification of the dye-electrolyte interaction.

PubMed

Ellis, Hanna; Leandri, Valentina; Hagfeldt, Anders; Boschloo, Gerrit; Bergquist, Jonas; Shevchenko, Denys

2015-05-01

Dye-sensitized solar cells (DSCs) have great potential to provide sustainable electricity from sunlight. The photoanode in DSCs consists of a dye-sensitized metal oxide film deposited on a conductive substrate. This configuration makes the photoanode a perfect sample for laser desorption/ionization mass spectrometry (LDI-MS). We applied LDI-MS for the study of molecular interactions between a dye and electrolyte on the surface of a TiO2 photoanode. We found that a dye containing polyoxyethylene groups forms complexes with alkali metal cations from the electrolyte, while a dye substituted with alkoxy groups does not. Guanidinium ion forms adducts with neither of the two dyes. Copyright © 2015 John Wiley & Sons, Ltd.

Intraluminal tissue welding for anastomosis

DOEpatents

Glinsky, M.; London, R.; Zimmerman, G.; Jacques, S.

1998-10-27

A method and device are provided for performing intraluminal tissue welding for anastomosis of a hollow organ. A retractable catheter assembly is delivered through the hollow organ and consists of a catheter connected to an optical fiber, an inflatable balloon, and a biocompatible patch mounted on the balloon. The disconnected ends of the hollow organ are brought together on the catheter assembly, and upon inflation of the balloon, the free ends are held together on the balloon to form a continuous channel while the patch is deployed against the inner wall of the hollow organ. The ends are joined or ``welded`` using laser radiation transmitted through the optical fiber to the patch. A thin layer of a light-absorbing dye on the patch can provide a target for welding. The patch may also contain a bonding agent to strengthen the bond. The laser radiation delivered has a pulse profile to minimize tissue damage. 8 figs.

Intraluminal tissue welding for anastomosis

DOEpatents

Glinsky, Michael; London, Richard; Zimmerman, George; Jacques, Steven

1998-10-27

A method and device are provided for performing intraluminal tissue welding for anastomosis of a hollow organ. A retractable catheter assembly is delivered through the hollow organ and consists of a catheter connected to an optical fiber, an inflatable balloon, and a biocompatible patch mounted on the balloon. The disconnected ends of the hollow organ are brought together on the catheter assembly, and upon inflation of the balloon, the free ends are held together on the balloon to form a continuous channel while the patch is deployed against the inner wall of the hollow organ. The ends are joined or "welded" using laser radiation transmitted through the optical fiber to the patch. A thin layer of a light-absorbing dye on the patch can provide a target for welding. The patch may also contain a bonding agent to strengthen the bond. The laser radiation delivered has a pulse profile to minimize tissue damage.

Atraumatic laser treatment for laryngeal papillomatosis

NASA Astrophysics Data System (ADS)

McMillan, Kathleen; Pankratov, Michail M.; Wang, Zhi; Bottrill, Ian; Rebeiz, Elie E.; Shapshay, Stanley M.

1994-09-01

Ten to fifteen thousand new cases of recurrent respiratory papillomatosis (RRP) are diagnosed each year in the United States. RRP is caused by the human papillomavirus (HPV) and is characterized by recurrent, non-malignant, proliferative lesions of the larynx. Patients with RRP undergo numerous microsurgical procedures to remove laryngeal papilloma threatening airway patency and interfering with phonation. The standard surgical technique involves CO2 laser vaporization of laryngeal epithelium affected by the lesions, and requires general anesthesia. The pulsed dye laser operating at 585 nm has previously been demonstrated to be effective in clearing HPV lesions of the skin (verrucae). For treatment of RRP, the fiber- compatible pulsed dye laser radiation may be delivered under local anesthesia using a flexible intranasal laryngoscope. Potential advantages of the pulsed dye laser treatment over CO2 laser surgery include (1) reduced morbidity, especially a lower risk of laryngeal scarring; (2) lower cost; (3) reduced technical difficulty; and (4) reduced risk of viral dissemination or transmission. In vivo studies are underway to determine the effect of pulsed dye laser radiation on normal canine laryngeal tissue.

Organic Solid-State Tri-Wavelength Lasing from Holographic Polymer-Dispersed Liquid Crystal and a Distributed Feedback Laser with a Doped Laser Dye and a Semiconducting Polymer Film.

PubMed

Liu, Minghuan; Liu, Yonggang; Peng, Zenghui; Wang, Shaoxin; Wang, Qidong; Mu, Quanquan; Cao, Zhaoliang; Xuan, Li

2017-05-07

Organic solid-state tri-wavelength lasing was demonstrated from dye-doped holographic polymer-dispersed liquid crystal (HPDLC) distributed feedback (DFB) laser with semiconducting polymer poly[-methoxy-5-(2'-ethyl-hexyloxy)-1,4-phenylene-vinylene] (MEH-PPV) and laser dye [4-(dicyanomethylene)-2-methyl-6-(p-dimethylaminostyryl)-4H-pyran] (DCM) by a one-step holography technique, which centered at 605.5 nm, 611.9 nm, and 671.1 nm. The temperature-dependence tuning range for the tri-wavelength dye-doped HPDLC DFB laser was as high as 8 nm. The lasing emission from the 9th order HPDLC DFB laser with MEH-PPV as active medium was also investigated, which showed excellent s-polarization characterization. The diffraction order is 9th and 8th for the dual-wavelength lasing with DCM as the active medium. The results of this work provide a method for constructing the compact and cost-effective all solid-state smart laser systems, which may find application in scientific and applied research where multi-wavelength radiation is required.

Electrophoretic mobility patterns of collagen following laser welding

NASA Astrophysics Data System (ADS)

Bass, Lawrence S.; Moazami, Nader; Pocsidio, Joanne O.; Oz, Mehmet C.; LoGerfo, Paul; Treat, Michael R.

1991-06-01

Clinical application of laser vascular anastomosis in inhibited by a lack of understanding of its mechanism. Whether tissue fusion results from covalent or non-covalent bonding of collagen and other structural proteins is unknown. We compared electrophoretic mobility of collagen in laser treated and untreated specimens of rat tail tendon (>90% type I collagen) and rabbit aorta. Welding was performed, using tissue shrinkage as the clinical endpoint, using the 808 nm diode laser (power density 14 watts/cm2) and topical indocyanine green dye (max absorption 805 nm). Collagen was extracted with 8 M urea (denaturing), 0.5 M acetic acid (non-denaturing) and acetic acid/pepsin (cleaves non- helical protein). Mobility patterns on gel electrophoresis (SDS-PAGE) after urea or acetic acid extraction were identical in the lasered and control tendon and vessel (confirmed by optical densitometry), revealing no evidence of formation of novel covalent bonds. Alpha and beta band intensity was diminished in pepsin incubated lasered specimens compared with controls (optical density ratio 0.00 +/- 9 tendon, 0.65 +/- 0.12 aorta), indicating the presence of denatured collagen. With the laser parameters used, collagen is denatured without formation of covalent bonds, suggesting that non-covalent interaction between denatured collagen molecules may be responsible for the weld. Based on this mechanism, welding parameters can be chosen which produce collagen denaturation without cell death.

A >2-MJ, 1014-W laser system for DT fusion—NIF: a note in celebration of the 75th birthday of Prof. Theodore Haensch

NASA Astrophysics Data System (ADS)

Holzrichter, John F.; Manes, Kenneth R.

2017-01-01

In 1970, Dr. Theodore Haensch joined A.L. Schawlow's group in the physics department at Stanford, as a NATO postdoctoral researcher. Within a short time, he and his colleagues had invented a new, high-resolution, tunable laser system using expanded reflection gratings and an N2 laser for pumping the fluorescing dyes. This work resulted in a high-brightness, high-repetition-rate, narrow-band laser probe for conducting optical spectroscopy at extreme levels of precision. Dr. Haensch, and his many colleagues, particularly Prof. Arthur Schawlow and their students at Stanford, then proceeded to revolutionize optical spectroscopy and to train several generations of exceptional young scientists. At the same time, the Siegman, Harris, and Byer laboratories also at Stanford were making major contributions to the laser and quantum electronics fields. Several students from both groups joined the Livermore Laboratory. That early work, and that of others, encouraged teams at the Lawrence Livermore National Laboratory to design and build a series of increasing complicated, high-power multi-beam laser systems to investigate the potential of laser fusion. The National Ignition Facility, recently completed, is enabling investigations of matter at very high temperatures, T > 1 million K and densities 100-1000× normal. In addition, researchers are creating 1015 DT fusion neutrons per fusion experiment and generating new knowledge about unusual and important conditions of matter.

Fractional Resurfacing Aiding Photodynamic Therapy of a Recalcitrant Plantar Verruca

PubMed Central

Pope, Amy

2008-01-01

Fractional resurfacing has become a very popular laser modality in recent years, and photodynamic therapy (PDT) has become a mainstay of many practices treating a wide array of clinical entities. In this case report, we describe a recalcitrant verrucous lesion on the foot that is unresponsive to cryotherapy, pulsed dye laser, and pulsed dye laser with PDT. The lesion did, however, respond very well to the use of a fractional laser to enhance the penetration of the PDT photosensitizer and then responded to pulsed dye laser with PDT. Fractional resurfacing prior to PDT may be a novel dermatologic treatment approach, making PDT an even better treatment option in the future. PMID:21103307

Laser Microdissection and Atmospheric Pressure Chemical Ionization Mass Spectrometry Coupled for Multimodal Imaging

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lorenz, Matthias; Ovchinnikova, Olga S; Kertesz, Vilmos

2013-01-01

This paper describes the coupling of ambient laser ablation surface sampling, accomplished using a laser capture microdissection system, with atmospheric pressure chemical ionization mass spectrometry for high spatial resolution multimodal imaging. A commercial laser capture microdissection system was placed in close proximity to a modified ion source of a mass spectrometer designed to allow for sampling of laser ablated material via a transfer tube directly into the ionization region. Rhodamine 6G dye of red sharpie ink in a laser etched pattern as well as cholesterol and phosphatidylcholine in a cerebellum mouse brain thin tissue section were identified and imaged frommore » full scan mass spectra. A minimal spot diameter of 8 m was achieved using the 10X microscope cutting objective with a lateral oversampling pixel resolution of about 3.7 m. Distinguishing between features approximately 13 m apart in a cerebellum mouse brain thin tissue section was demonstrated in a multimodal fashion including co-registered optical and mass spectral chemical images.« less

Design of intelligent mesoscale periodic array structures utilizing smart hydrogel

NASA Technical Reports Server (NTRS)

Sunkara, H. B.; Penn, B. G.; Frazier, D. O.; Weissman, J. M.; Asher, S. A.

1996-01-01

Mesoscale Periodic Array Structures (MPAS, also known as crystalline colloidal arrays), composed of aqueous or nonaqueous dispersions of self-assembled submicron colloidal spheres are emerging toward the development of advanced optical devices for technological applications. This is because of their unique optical diffraction properties and the ease with which these intriguing properties can be modulated experimentally. Moreover our recent advancements in this area which include 'locking' the liquid MPAS into solid or semisolid polymer matrices for greater stability with longer life span, and incorporation of CdS quantum dots and laser dyes into colloidal spheres to obtain nonlinear optical (NLO) responses further corroborate the use of MPAS in optical technology. Our long term goal is fabrication of all-optical and electro-optical devices such as spatial light modulators for optical signal processing and flat panel display devices by utilizing intelligent nonlinear periodic array structural materials. Here we show further progress in the design of novel linear MPAS which have the ability to sense and respond to an external source such as temperature. This is achieved by combining the self-assembly properties of polymer colloidal spheres and thermoshrinking properties of smart polymer gels. At selected temperatures the periodic array efficiently Bragg diffracts light and transmits most of the light at other temperatures. Hence these intelligent systems are of potential use as fixed notch filters optical switches or limiters to protect delicate optical sensors from high intensity laser radiation.

Balloon borne in-situ detection of OH in the stratosphere from 37 to 23 km

NASA Technical Reports Server (NTRS)

Stimpfle, R. M.; Lapson, L. B.; Wennberg, P. O.; Anderson, J. G.

1989-01-01

The OH number density in the stratosphere has been measured over the altitude interval of 37 to 23 km at midday via a balloon-borne gondola launched from Palestine, Texas on July 6, 1988. OH radicals are detected with a laser-induced fluorescence instrument employing a 17-kHz-repetition-rate copper vapor laser-pumped dye laser optically coupled to an enclosed flow, in-situ sampling chamber. OH abundances ranged from 88 + or - 3l pptv in the 36 to 35 km interval to 0.9 + or - 0.8 pptv in the 24 to 23 km interval. The stated uncertainty includes that from both measurement precision and accuracy. Simultaneous detection of ozone and water vapor densities was carried out with separate on-board instruments.

Nanospectrofluorometry inside single living cell by scanning near-field optical microscopy

NASA Astrophysics Data System (ADS)

Lei, F. H.; Shang, G. Y.; Troyon, M.; Spajer, M.; Morjani, H.; Angiboust, J. F.; Manfait, M.

2001-10-01

Near-field fluorescence spectra with subdiffraction limit spatial resolution have been taken in the proximity of mitochondrial membrane inside breast adenocarcinoma cells (MCF7) treated with the fluorescent dye (JC-1) by using a scanning near-field optical microscope coupled with a confocal laser microspectrofluorometer. The probe-sample distance control is based on a piezoelectric bimorph shear force sensor having a static spring constant k=5 μN/nm and a quality factor Q=40 in a physiological medium of viscosity η=1.0 cp. The sensitivity of the force sensor has been tested by imaging a MCF7 cell surface.

Optics with Semiconductors: Ultrafast Physics for Devices

DTIC Science & Technology

1991-03-01

pass through a thinner (ɛÜ0 /im) saturable absorber jet of malachite green placed between the fourth and fifth passes. The saturable absorber reduces...this laser [22]. In the amplifier, the gain/absorber dye pair is SR640/ malachite green, after Knox [8]. SPli Vol 126$ Appttctiom of Ukrtshon Lssf...telescope, followed by a short tele- scope with a saturable absorber at its focus ( malachite green in a ~200 pm jet of ethylene glycol, produced by

Optical properties of stilbene-type dyes containing various terminal donor and acceptor groups

NASA Astrophysics Data System (ADS)

Qin, Chuanxiang; Zhang, Weizhou; Wang, Zhiming; Zhou, Maoyi; Wang, Xiaomei; Chen, Guoqiang

2008-06-01

A series of "D-π-A" stilbene-type dyes, named as trans-4-[p-(N,N-hydroxyethyl) aminino-styryl]-N-methylpyridinium iodide (DHEASPI-C1), trans-4-[p-(N,N-hydroxyethyl) aminino-styryl]-N-butylpyridinium bromide (DHEASPBr-C4), trans-4-[p-(N,N-hydroxyethyl) aminino-styryl]-N-octylpyridinium bromide (DHEASPBr-C8) and trans-4-[p-(N,N-hydroxyethyl) aminino-styryl]-N-dodecylpyridinium bromide (DHEASPBr-C12), respectively, have been synthesized and their optical properties have been experimentally investigated. When DHEASPI-C1 are compared with trans-4-[p-(N,N-diethylamino) styryl]-N-methylpyridinium iodide (DEASPI) and trans-4-[p-(N-hydroxyethyl-N-ethylamino) styryl]-N-methylpyridinium iodide (HEASPI), both the electronegativity of two hydroxyl and intra-molecular hydrogen bond decrease the donor ability of the ethyl chain, there are obvious blue shifts both in single-photon absorption spectra, fluorescence spectra and two-photon excited fluorescence spectra. Interestingly, fluorescence intensity of DHEASPI-C1 is the biggest. There are little shifts from DHEASPBr-C4 to DHEASPBr-C8 and to DHEASPBr-C12 in their spectra. As dyes' two-photon excited fluorescence spectra were concerned, pumped by 1064 nm, <130 fs mode-locked Nd:YAG laser, their peak locations were between 613 and 623 nm.

Ultrafast Laser Techniques

DTIC Science & Technology

1991-06-05

2 Prism Dye Amplifiers .................................................................................. 2 Axicon...carried out under this project. PRISM DYE AMPLIFIERS A first effort was devoted to setting up an amplifier system for the output of a short pulse dye laser...For amplification up to pulse energies of approximately 500 p.J/pulse we chose three stages of prism amplifier cells, with diameters of 1 m, 3 mm

In vitro study of bactericidal effect of low-level laser therapy in the presence of photosensitizer on cariogenic bacteria

NASA Astrophysics Data System (ADS)

Zanin, Iriana C. J.; Brugnera, Aldo, Jr.; Goncalves, Reginaldo B.

2002-06-01

The aim of this in vitro study was to determine whether low-level laser light in the presence of a photosensitizer could kill Streptococcus mutans and Streptococcus sobrinus. Suspensions of these microorganisms were exposed to a gallium-aluminium-arsenide laser light (660 nm) in the presence of photosensitizer toluidine blue O. Viable microorganisms were counted on brain heart agar plates after incubation at 37 degree(s)C in partial atmosphere of 10% CO2 for 48 hours. Their exposure to the laser light in the absence of the dye or the dye in the absence of the laser light presented no significant effect on the viability of the microorganisms. However, a decrease in the number of viable microorganisms was only verified when they were exposed to both the laser light and the dye at the same time. Their total growth inhibition was achieved with a dye concentration of 100 mg/mL and a light energy density of 28.8 J/cm2, after being exposed to laser light for 900 seconds. In conclusion, these results imply that these bacteria can be killed by low-power laser light in the presence of the photosensitizer.

Passive mode locking of a Nd:YAG laser with a thin gelatine-film saturable absorber containing organic-dye J-aggregates

DOE Office of Scientific and Technical Information (OSTI.GOV)

Avdeeva, V I; Shapiro, Boris I; Kuch'yanov, Aleksandr S

2003-06-30

Ultrashort pulses of duration {approx}13 ps are first obtained in a passively mode-locked Nd:YAG laser with a negative feedback using a thin gelatine-film saturable absorber containing organic-dye J-aggregates. (control of laser radiation parameters)

Pulsed dye laser application in ablation of vascular ectasias of the larynx: a preliminary animal study

NASA Astrophysics Data System (ADS)

Woo, Peak; Wang, Zhi; Perrault, Donald F., Jr.; McMillan, Kathleen; Pankratov, Michail M.

1995-05-01

Vascular ectasias (dilatation) and vascular lesions of the larynx are difficult to treat with exciting modalities. Varix (enlarged vessel) of the vocal folds, vocal fold hemorrhage, vascular polyp, hemangioma, intubation or contact granuloma are common problems which disturb voice. Current applications of CO2 laser and cautery often damage the delicate vocal fold cover. The 585 nm dermatologic pulsed dye laser may be an ideal substitute. Two adult canines were examined under anesthesia via microlaryngoscopy technique. Pulsed dye laser (SPTL-1a, Candela Laser Corp., Wayland, MA) energy was delivered via the micromanipulator with the 3.1-mm spot size in single pulses of 6, 8, and 10 Joules/cm2 and applied to the vessels of the vocal folds, epiglottis, and arytenoid cartilage. Endoscopic examination was carried out immediately after the treatment and at 4 weeks postoperatively. The animals were sacrificed at 3 weeks, larynges excised, and whole organ laryngeal section were prepared for histology. Pulsed dye laser thrombosed vessels of the vocal fold using 6 or 8 Joules/cm2. Vascular break and leakage occurred at 10 Joules/cm2. Follow up examination showed excellent vessel obliteration or thrombosis without scarring or injury to the overlying tissues. Histologic examination shows vascular thrombosis without inflammation and fibrosis in the vocal fold cover. Pulsed dye laser may have promise in treatment of vascular lesions of the larynx and upper airway.

Effects of 532 nm pulsed-KTP laser parameters on vessel ablation in the avian chorioallantoic membrane: implications for vocal fold mucosa.

PubMed

Broadhurst, Matthew S; Akst, Lee M; Burns, James A; Kobler, James B; Heaton, James T; Anderson, R Rox; Zeitels, Steven M

2007-02-01

Selective vascular ablation (photoangiolysis) using pulsed lasers that target hemoglobin is an effective treatment strategy for many vocal fold lesions. However, vessel rupture with extravasation of blood reduces selectivity for vessels, which is frequently observed with the 0.45-ms, 585-nm pulsed dye laser. Previous studies have shown that vessel rupture is the result of vaporization of blood, an event that varies with laser pulse width and pulse fluence (energy per unit area). Clinical observations using a 532-nm wavelength pulsed potassium-titanyl-phosphate (KTP) laser revealed less laser-induced hemorrhage than the pulsed dye laser. This study investigated settings for the pulsed KTP laser to achieve selective vessel destruction without rupture using the avian chorioallantoic membrane under conditions similar to flexible laryngoscopic delivery of the laser in clinical practice. The chick chorioallantoic membrane offers convenient access to many small blood vessels similar in size to those targeted in human vocal fold. Using a 532-nm pulsed KTP laser, pulse width, pulse energy, and working distance from the optical delivery fiber were varied to assess influence on the ability to achieve vessel coagulation without vessel wall rupture. Third-order vessels (n = 135) were irradiated: Energy (471-550 mJ), pulse width (10, 15, 30 ms), and fiber-to-tissue distance (1 mm, 3 mm) were varied systematically. Selective vessel destruction without vessel wall rupture was more often achieved by increasing pulse width, increasing the fiber-to-tissue distance, and decreasing energy. Vessel destruction without rupture was consistently achieved using 15- or 30-ms pulses with a fiber-to-tissue distance of 3 mm (pulse fluence of 13-16 J/cm). This study substantiates our clinical observation that a 532-nm pulsed KTP laser was effective for ablating microcirculation while minimizing vessel wall rupture and hemorrhage.

Sub-wavelength plasmon laser

DOEpatents

Bora, Mihail; Bond, Tiziana C.

2016-04-19

A plasmonic laser device has resonant nanocavities filled with a gain medium containing an organic dye. The resonant plasmon frequencies of the nanocavities are tuned to align with both the absorption and emission spectra of the dye. Variables in the system include the nature of the dye and the wavelength of its absorption and emission, the wavelength of the pumping radiation, and the resonance frequencies of the nanocavities. In addition the pumping frequency of the dye is selected to be close to the absorption maximum.

In vitro double-integrating-sphere optical properties of tissues between 630 and 1064 nm

NASA Astrophysics Data System (ADS)

Beek, J. F.; Blokland, P.; Posthumus, P.; Aalders, M.; Pickering, J. W.; Sterenborg, H. J. C. M.; van Gemert, M. J. C.

1997-11-01

The optical properties (absorption and scattering coefficients and the scattering anisotropy factor) were measured in vitro for cartilage, liver, lung, muscle, myocardium, skin, and tumour (colon adenocarcinoma CC 531) at 630, 632.8, 790, 850 and 1064 nm. Rabbits, rats, piglets, goats, and dogs were used to obtain the tissues. A double-integrating-sphere setup with an intervening sample was used to determine the reflectance, and the diffuse and collimated transmittances of the sample. The inverse adding - doubling algorithm was used to determine the optical properties from the measurements. The overall results were comparable to those available in the literature, although only limited data are available at 790 - 850 nm. The results were reproducible for a specific sample at a specific wavelength. However, when comparing the results of different samples of the same tissue or different lasers with approximately the same wavelength (e.g. argon dye laser at 630 nm and HeNe laser at 632.8 nm) variations are large. We believe these variations in optical properties should be explained by biological variations of the tissues. In conclusion, we report on an extensive set of in vitro absorption and scattering properties of tissues measured with the same equipment and software, and by the same group. Although the accuracy of the method requires further improvement, it is highly likely that the other existing data in the literature have a similar level of accuracy.

Enhanced magnetic Purcell effect in room-temperature masers

PubMed Central

Breeze, Jonathan; Tan, Ke-Jie; Richards, Benjamin; Sathian, Juna; Oxborrow, Mark; Alford, Neil McN

2015-01-01

Recently, the world’s first room-temperature maser was demonstrated. The maser consisted of a sapphire ring housing a crystal of pentacene-doped p-terphenyl, pumped by a pulsed rhodamine-dye laser. Stimulated emission of microwaves was aided by the high quality factor and small magnetic mode volume of the maser cavity yet the peak optical pumping power was 1.4 kW. Here we report dramatic miniaturization and 2 orders of magnitude reduction in optical pumping power for a room-temperature maser by coupling a strontium titanate resonator with the spin-polarized population inversion provided by triplet states in an optically excited pentacene-doped p-terphenyl crystal. We observe maser emission in a thimble-sized resonator using a xenon flash lamp as an optical pump source with peak optical power of 70 W. This is a significant step towards the goal of continuous maser operation. PMID:25698634

AFRL Advanced Electric Lasers Branch - Construction and Upgrade of a 50-watt Facility-Class Sodium Guidestar Pump Laser

NASA Astrophysics Data System (ADS)

Bronder, T.; Miller, H.; Stohs, J.; Lu, C.; Baker, J.; Lucero, A.

The development of a reliable and effective laser source for pumping mesospheric sodium to generate an artificial guidestar has been well documented. From the early achievements with 589nm high-power dye lasers at the Keck and Lick observatories to the ground-breaking 50W CW FASOR (Frequency Addition Source of Optical Radiation) Guidestar at the Air Forces Starfire Optical Range (SOR), there has been intense interest in this technology from both the academic and military communities. Beginning in the fall of 2008, the Air Force Research Laboratorys Advanced Electric Lasers Branch began a project to build, test, verify and deliver an upgraded version of the SOR FASOR for use at the AF Maui Optical Station (AMOS) in the summer of 2010. This FASOR will be similar in design to the existing SOR device and produce 50W of diffraction limited, linearly polarized narrow linewidth 589nm light by combining the output of two injection-locked Nd:YAG ring lasers (operating at 1064nm and 1319nm) using resonant sum-frequency generation in a lithium triborate crystal (LBO). The upgraded features will include modularized sub-components, embedded control electronics, and a simplified cooling system. The first portion of this upgrade project is to reconstruct the current SOR FASOR components and include improved methods of regulating the gain modules of the two injection lasers. In parallel with this effort, the technical plans for the modularization and re-packaging of the FASOR will be finalized and coordinated with the staff at Maui. This presentation will summarize the result of these efforts to date and provide updates on the AMOS FASOR status. Additionally, plans for "next-generation" FASOR upgrades for both SOR and AMOS will also be discussed.

Design of smart optical sensor using polyvinyl alcohol/Fluorescein sodium salt: Laser filters and optical limiting effect

NASA Astrophysics Data System (ADS)

Yahia, I. S.; Bouzidi, A.; Zahran, H. Y.; Jilani, W.; AlFaify, S.; Algarni, H.; Guermazi, H.

2018-03-01

Pure poly (vinyl alcohol) (PVA) and PVA doped Fluorescein-Sodium salt (FSS/PVA composite films) have synthesized on wide scale laser optical filters. The investigated polymeric composite films have been characterized using several methods. The XRD patterns exhibit a decrease of the average crystalline size and an increase of the internal strain, which explained the imperfection and distortion in the prepared films. The optical characterizations showed a decrease in the transmission of the incident light for different samples, which may be explained to the layer formed by intermolecular hydrogen bonding between the PVA matrix and the FSS particles. The FSS/PVA polymeric composite films are being a completely blocking in the UV-Vis light at the range between 190 and 560 nm, agreement with the optical limiting effect, which makes the composite films suitable for CUT-OFF laser filters applications. The decrease in its, directly and indirectly, allowed transition band gaps were controlled by the added FSS dyes molecules. The variation of the exponent frequency (s) of the power law for FSS/PVA polymeric composite films has been characterized to improve the hopping conduction mechanism in the materials. The dielectric permittivity (e‧) and dielectric loss (e'') have been decreased with increasing the applied frequency, and the incorporated FSS molecules due to the DC electric conductivity can cause the decreases of the polarization of the as-prepared films over the studied ranges.

Dye laser traveling wave amplifier

NASA Technical Reports Server (NTRS)

Davidson, F.; Hohman, J.

1985-01-01

Injection locking was applied to a cavity-dumped coaxial flashlamp pumped dye laser in an effort to obtain nanosecond duration pulses which have both high energy and narrow-linewidth. In the absence of an injected laser pulse, the cavity-dumped dye laser was capable of generating high energy (approx. 60mJ) nanosecond duration output pulses. These pulses, however, had a fixed center wavelength and were extremely broadband (approx. 6nm FWHM). Experimental investigations were performed to determine if the spectral properties of these outputs could be improved through the use of injection-locking techniques. A parametric study to determine the specific conditions under which the laser could be injection-locked was also carried out. Significant linewidth reduction to 0.0015nm) of the outputs was obtained through injection-locking but only at wavelengths near the peak lasing wavelength of the dye. It was found, however; that by inserting weakly dispersive tuning elements in the laser cavity, these narrow-linewidth outputs could be obtained over a wide (24nm) tuning range. Since the tuning elements had low insertion losses, the tunability of the output was obtained without sacrificing output pulse energy.

Amplified emission and modified spectral features in an opal hetero-structure mediated by passive defect mode localization

NASA Astrophysics Data System (ADS)

Rout, Dipak; Kumar, Govind; Vijaya, R.

2018-01-01

A photonic crystal hetero-structure consisting of a passive planar defect of SiO2 thin film sandwiched between two identical opals grown by inward growing self-assembly method using Rhodamine-B dye-doped polystyrene microspheres is studied for the characteristics of dye emission. The optical properties and the defect mode characteristics of the hetero-structure are studied from the reflection and transmission measurements. Laser-induced fluorescence from the hetero-structure showed amplified and spectrally narrowed emission compared to the photonic crystal emphasizing the role of the defect mode and distributed feedback. The enhanced emission is also complemented by the reduction in fluorescence decay time in the case of the hetero-structure in comparison to the 3D photonic crystals.

Photodynamic dye adsorption and release performance of natural zeolite

PubMed Central

Hovhannisyan, Vladimir; Dong, Chen-Yuan; Chen, Shean-Jen

2017-01-01

Clinoptilolite type of zeolite (CZ) is a promising material for biomedicine and pharmaceutics due to its non-toxicity, thermal stability, expanded surface area, and exceptional ability to adsorb various atoms and organic molecules into micropores. Using multiphoton microscopy, we demonstrated that individual CZ particles produce two-photon excited luminescence and second harmonic generation signal at femtosecond laser excitation, and adsorb photo-dynamically active dyes such as hypericin and methylene blue. Furthermore, the release of hypericin from CZ pores in the presence of biomolecules is shown, and CZ can be considered as an effective material for drug delivery and controlled release in biological systems. The results may open new perspectives in application of CZ in biomedical imaging, and introducing of the optical approaches into the clinical environment. PMID:28361968

Temporal measurement on and using pulses from spectrally narrowed emission in styrylpyridinium cyanine dye

NASA Astrophysics Data System (ADS)

Dharmadhikari, Aditya K.; Bhowmik, Achintya K.; Ahyi, Ayayi C.; Thakur, Mrinal

2001-11-01

Highly efficient spectrally narrowed emission (SNE) was observed in the solution of strylpyridinium cyanine dye (SPCD) pumped by fundamental and second harmonic of a picosecond Nd:YAG laser in two separate arrangements. A highly directional emission was observed in both the pumping arrangements without incorporating any mirrors. The pulse duration of the SNE was measured by background free SHG intensity autocorrelation technique. The measured duration of the pulses was 40 ps. These pulses, having a spectral linewidth of 10 nm (full width at half maximum), were used as a probe to measure the transient changes in the transmission in SPCD solution using a pump-probe setup. The transient optical transmission indicated a gain at the overlap and no gain was observed beyond a delay of 40 ps.

All-polymer photonic sensing platform based on whispering-gallery mode microgoblet lasers.

PubMed

Wienhold, T; Kraemmer, S; Wondimu, S F; Siegle, T; Bog, U; Weinzierl, U; Schmidt, S; Becker, H; Kalt, H; Mappes, T; Koeber, S; Koos, C

2015-09-21

We present an all-polymer photonic sensing platform based on whispering-gallery mode microgoblet lasers integrated into a microfluidic chip. The chip is entirely made from polymers, enabling the use of the devices as low-cost disposables. The microgoblet cavities feature quality factors exceeding 10(5) and are fabricated from poly(methyl methacrylate) (PMMA) using spin-coating, mask-based optical lithography, wet chemical etching, and thermal reflow. In contrast to silica-based microtoroid resonators, this approach replaces technically demanding vacuum-based dry etching and serial laser-based reflow techniques by solution-based processing and parallel thermal reflow. This enables scaling to large-area substrates, and hence significantly reduces device costs. Moreover, the resonators can be fabricated on arbitrary substrate materials, e.g., on transparent and flexible polymer foils. Doping the microgoblets with the organic dye pyrromethene 597 transforms the passive resonators into lasers. Devices have lasing thresholds below 0.6 nJ per pulse and can be efficiently pumped via free-space optics using a compact and low-cost green laser diode. We demonstrate that arrays of microgoblet lasers can be readily integrated into a state-of-the-art microfluidic chip replicated via injection moulding. In a proof-of-principle experiment, we show the viability of the lab-on-a-chip via refractometric sensing, demonstrating a bulk refractive index sensitivity (BRIS) of 10.56 nm per refractive index unit.

Scanning fluorescence detector for high-throughput DNA genotyping

NASA Astrophysics Data System (ADS)

Rusch, Terry L.; Petsinger, Jeremy; Christensen, Carl; Vaske, David A.; Brumley, Robert L., Jr.; Luckey, John A.; Weber, James L.

1996-04-01

A new scanning fluorescence detector (SCAFUD) was developed for high-throughput genotyping of short tandem repeat polymorphisms (STRPs). Fluorescent dyes are incorporated into relatively short DNA fragments via polymerase chain reaction (PCR) and are separated by electrophoresis in short, wide polyacrylamide gels (144 lanes with well to read distances of 14 cm). Excitation light from an argon laser with primary lines at 488 and 514 nm is introduced into the gel through a fiber optic cable, dichroic mirror, and 40X microscope objective. Emitted fluorescent light is collected confocally through a second fiber. The confocal head is translated across the bottom of the gel at 0.5 Hz. The detection unit utilizes dichroic mirrors and band pass filters to direct light with 10 - 20 nm bandwidths to four photomultiplier tubes (PMTs). PMT signals are independently amplified with variable gain and then sampled at a rate of 2500 points per scan using a computer based A/D board. LabView software (National Instruments) is used for instrument operation. Currently, three fluorescent dyes (Fam, Hex and Rox) are simultaneously detected with peak detection wavelengths of 543, 567, and 613 nm, respectively. The detection limit for fluorescein-labeled primers is about 100 attomoles. Planned SCAFUD upgrades include rearrangement of laser head geometry, use of additional excitation lasers for simultaneous detection of more dyes, and the use of detector arrays instead of individual PMTs. Extensive software has been written for automatic analysis of SCAFUD images. The software enables background subtraction, band identification, multiple- dye signal resolution, lane finding, band sizing and allele calling. Whole genome screens are currently underway to search for loci influencing such complex diseases as diabetes, asthma, and hypertension. Seven production SCAFUDs are currently in operation. Genotyping output for the coming year is projected to be about one million total genotypes (DNA samples X polymorphic markers) at a total cost of

Development of New Laser Protective Dyes

DTIC Science & Technology

1993-07-31

Science’s Phase I research, the feasibility of thermally stabilizing cyanine and squarylium dyes for simulated polycarbonate injection-molding... SQUARYLIUM & CROCONIUM FIUORENE DYE SYNTHESIS SYNTHESIS OF NEW DYES DYE SYNTHESES IENGFICA TION ASYNTHESIS OF SUSSTITUTED DYES EVELOP OH TECHNOLOGIES...region, three dyes were successfully extruded into PETG and/or PC: (a.) the croconium dye SS-1044 (,%max = 834 um in PETG). (b.) the squarylium dye

A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Wenbo; Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8; Department of Biomedical Engineering, University of British Columbia, KAIS 5500, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4

Scanning speed and coupling efficiency of excitation light to optic fibres are two major technical challenges that limit the potential of fluorescence excitation-emission matrix (EEM) spectrometer for on-line applications and in vivo studies. In this paper, a novel EEM system, utilizing a supercontinuum white light source and acousto-optic tunable filters (AOTFs), was introduced and evaluated. The supercontinuum white light, generated by pumping a nonlinear photonic crystal fiber with an 800 nm femtosecond laser, was efficiently coupled into a bifurcated optic fiber bundle. High speed EEM spectral scanning was achieved using AOTFs both for selecting excitation wavelength and scanning emission spectra.more » Using calibration lamps (neon and mercury argon), wavelength deviations were determined to vary from 0.18 nm to −0.70 nm within the spectral range of 500–850 nm. Spectral bandwidth for filtered excitation light broadened by twofold compared to that measured with monochromatic light between 650 nm and 750 nm. The EEM spectra for methanol solutions of laser dyes were successfully acquired with this rapid fluorometer using an integration time of 5 s.« less

Molecular engineering of cyanine dyes to design a panchromatic response in Co-sensitized dye-sensitized solar cells

DOE PAGES

Pepe, Giulio; Cole, Jacqueline M.; Waddell, Paul G.; ...

2016-04-05

Cyanines are optically tunable dyes with high molar extinction coefficients, suitable for applications in co-sensitized dye-sensitized solar cells (DSCs); yet, barely thus applied. This might be due to the lack of a rational molecular design strategy that efficiently exploits cyanine properties. This study computationally re-designs these dyes, to broaden their optical absorption spectrum and create dye···TiO 2 binding and co-sensitization functionality. This is achieved via a stepwise molecular engineering approach. Firstly, the structural and optical properties of four parent dyes are experimentally and computationally investigated: 3,3’-diethyloxacarbocyanine iodide, 3,3’-diethylthiacarbocyanine iodide, 3,3’-diethylthiadicarbocyanine iodide and 3,3’-diethylthiatricarbocyanine iodide. Secondly, the molecules are theoretically modifiedmore » and their energetics are analyzed and compared to the parent dyes. A dye···TiO 2 anchoring group (carboxylic or cyanoacrylic acid), absent from the parent dyes, is chemically substituted at different molecular positions to investigate changes in optical absorption. We find that cyanoacrylic acid substitution at the para-quinoidal position affects the absorption wavelength of all parent dyes, with an optimal bathochromic shift of ca. 40 nm. The theoretical lengthening of the polymethine chain is also shown to effect dye absorption. Two molecularly engineered dyes are proposed as promising co-sensitizers. Finally, corresponding dye···TiO 2 adsorption energy calculations corroborate their applicability, demonstrating the potential of cyanine dyes in DSC research.« less

Bibliography of Soviet Laser Developments Number 57, January-February 1982.

DTIC Science & Technology

1983-03-02

1 3. Crystal: Miscellaneous ............. 2 4. Semiconductor a. InP..............................................3 b . Pbl...Nd...........................................6 6. Glass: Miscellaneous................................7 B . Liquid Lasers I. Organic Dyes a. Rhodamine...8 b . Miscellaneous Dyes...............................8 2. Incrganic Liquids

Visible light-activated immobilization of biomolecules on polymer substrates for bio-sensing applications

NASA Astrophysics Data System (ADS)

Sims, Wesley Daniel

This dissertation aims to add to the scientific knowledge of physics in the field of optics by investigating the feasibility to develop a novel technique for immobilization of dye- labeled biomolecules on a polymer substrate. The development of this platform could potentially be used for bio sensing of biohazards in food and the environment. The process is facilitated by excitation of a dye-label attached to the biomolecule of interest with visible light of 488 nm wavelength. Biomolecules from an aqueous medium can be attached at any desired spot on the substrate simply by exposing the area to light. The area of the focused laser beam can control the spot-size of immobilized biomolecules. The technique is used to fabricate microarrays of immobilized antibodies (immunomicroarray) having spot-size of the order of 1 micron. This is a significant improvement over the typical commercial microarrays with spot-size in 10-100 micron range. The immobilization technique is characterized by attaching phospholipids, which have been shown to be useful as platforms for bio sensing applications. It can further be developed by attaching common proteins like Avidin as well as other antibodies against toxins and pathogens known for potential bio-terrorism through food and water systems. Absorption of laser-excited dye labeled biomolecules within the polymer appears to be the mechanism for attachment technique.

Laser treatments of deep-seated brain lesions

NASA Astrophysics Data System (ADS)

Ward, Helen A.

1997-06-01

The five year survival rate of deep-seated malignant brain tumors after surgery/radiotherapy is virtually 100 percent mortality. Special problems include: (1) Lesions often present late. (2) Position: lesion overlies vital structures, so complete surgical/radiotherapy lesion destruction can damage vital brain-stem functions. (3) Difficulty in differentiating normal brain form malignant lesions. This study aimed to use the unique properties of the laser: (a) to minimize damage during surgical removal of deep-seated brain lesions by operating via fine optic fibers; and (b) to employ the propensity of certain lasers for absorption of dyes and absorption and induction of fluorescence in some brain substances, to differentiate borders of malignant and normal brain, for more complete tumor removal. In the method a fine laser endoscopic technique was devised for removal of brain lesions. The results of this technique, were found to minimize and accurately predict the extent of thermal damage and shock waves to within 1-2mm of the surgical laser beam. Thereby it eliminated the 'popcorn' effect.

Effects of viscosity on sperm motility studied with optical tweezers

NASA Astrophysics Data System (ADS)

Hyun, Nicholas; Chandsawangbhuwana, Charlie; Zhu, Qingyuan; Shi, Linda Z.; Yang-Wong, Collin; Berns, Michael W.

2012-02-01

The purpose of this study is to analyze human sperm motility and energetics in media with different viscosities. Multiple experiments were performed to collect motility parameters using customized computer tracking software that measures the curvilinear velocity (VCL) and the minimum laser power (Pesc) necessary to hold an individual sperm in an optical trap. The Pesc was measured by using a 1064 nm Nd:YVO4 continuous wave laser that optically traps motile sperm at a power of 450 mW in the focused trap spot. The VCL was measured frame by frame before trapping. In order to study sperm energetics under different viscous conditions sperm were labeled with the fluorescent dye DiOC6(3) to measure membrane potentials of mitochondria in the sperm midpiece. Fluorescence intensity was measured before and during trapping. The results demonstrate a decrease in VCL but an increase in Pesc with increasing viscosity. Fluorescent intensity is the same regardless of the viscosity level indicating no change in sperm energetics. The results suggest that, under the conditions tested, viscosity physically affects the mechanical properties of sperm motility rather than the chemical pathways associated with energetics.

Application of gold nanoparticles as contrast agents in confocal laser scanning microscopy

NASA Astrophysics Data System (ADS)

Lemelle, A.; Veksler, B.; Kozhevnikov, I. S.; Akchurin, G. G.; Piletsky, S. A.; Meglinski, I.

2009-01-01

Confocal laser scanning microscopy (CLSM) is a modern high-resolution optical technique providing detailed image of tissue structure with high (down to microns) spatial resolution. Aiming at a concurrent improvement of imaging depth and image quality the CLSM requires the use of contrast agents. Commonly employed fluorescent contrast agents, such as fluorescent dyes and proteins, suffer from toxicity, photo-bleaching and overlapping with the tissues autofluorescence. Gold nanoparticles are potentially highly attractive to be applied as a contrast agent since they are not subject to photo-bleaching and can target biochemical cells markers associated with the specific diseases. In current report we consider the applicability of gold nano-spheres as a contrast agent to enhance quality of CLSM images of skin tissues in vitro versus the application of optical clearing agent, such as glycerol. The enhancement of CLSM image contrast was observed with an application of gold nano-spheres diffused within the skin tissues. We show that optical clearing agents such as a glycerol provide better CLSM image contrast than gold nano-spheres.

Characteristics of color optical shutter with dye-doped polymer network liquid crystal.

PubMed

Lee, G H; Hwang, K Y; Jang, J E; Jin, Y W; Lee, S Y; Jung, J E

2011-03-01

The optical properties and the theoretical prediction of color optical shutter with dye-doped polymer network liquid crystal (PNLC) were investigated. The view-angle dependence of reflectance according to the bias conditions showed distinctive characteristics, which could be explained from the effects of dye absorption and path length. It was also shown that the thickness dependence of reflectance was strongly influenced by the light-scattering coefficient. Our experimental results matched up well with the theoretical prediction based on the light scattering of liquid crystals in polymer network and the absorption of dichroic dye. This work indicates potential to improve the optical device using dye-doped liquid crystal-polymer composite.

Combined optical gain and degradation measurements in DCM2 doped Tris-(8-hydroxyquinoline)aluminum thin-films

NASA Astrophysics Data System (ADS)

Čehovski, Marko; Döring, Sebastian; Rabe, Torsten; Caspary, Reinhard; Kowalsky, Wolfgang

2016-04-01

Organic laser sources offer the opportunity to integrate flexible and widely tunable lasers in polymer waveguide circuits, e.g. for Lab-on-Foil applications. Therefore, it is necessary to understand gain and degradation processes for long-term operation. In this paper we address the challenge of life-time (degradation) measurements of photoluminescence (PL) and optical gain in thin-film lasers. The well known guest-host system of aluminum-chelate Alq3 (Tris-(8-hydroxyquinoline)aluminum) as host material and the laser dye DCM2 (4-(Dicyanomethylene)-2- methyl-6-julolidyl-9-enyl-4H-pyran) as guest material is employed as laser active material. Sample layers have been built up by co-evaporation in an ultrahigh (UHV) vacuum chamber. 200nm thick films of Alq3:DCM2 with different doping concentrations have been processed onto glass and thermally oxidized silicon substrates. The gain measurements have been performed by the variable stripe length (VSL) method. This measurement technique allows to determine the thin-film waveguide gain and loss, respectively. For the measurements the samples were excited with UV irradiation (ƛ = 355nm) under nitrogen atmosphere by a passively Q-switched laser source. PL degradation measurements with regard to the optical gain have been done at laser threshold (approximately 3 μJ/cm2), five times above laser threshold and 10 times above laser threshold. A t50-PL lifetime of > 107 pulses could be measured at a maximum excitation energy density of 32 μJ/cm2. This allows for a detailed analysis of the gain degradation mechanism and therefore of the stimulated cross section. Depending on the DCM2 doping concentration C the stimulated cross section was reduced by 35 %. Nevertheless, the results emphasizes the necessity of the investigation of degradation processes in organic laser sources for long-term applications.

[Therapeutic indications for percutaneous laser in patients with vascular malformations and tumors].

PubMed

Labau, D; Cadic, P; Ouroussoff, G; Ligeron, C; Laroche, J-P; Guillot, B; Dereure, O; Quéré, I; Galanaud, J-P

2014-12-01

Lasers are increasingly used to treat vascular abnormalities. Indeed, this technique is non-invasive and allows a specific treatment. The aim of this review is to present some biophysical principles of the lasers, to describe the different sorts of lasers available for treatment in vascular medicine indications. Three principal lasers exist in vascular medicine: the pulsed-dye laser, for the treatment of superficial pink lesions, the NdYAG-KTP laser for purple and bigger lesions, and the NdYAG long pulse laser for even deeper and bigger vascular lesions. In vascular malformations, port wine stains can also be treated by pulsed-dye laser, KTP or NdYAG when they are old and thick. Telangiectasias are good indications for the three sorts of lasers, depending on their depth, color and size. Microcystic lymphatic malformations can be improved by laser treatment. Arterio-venous malformations constitute a contraindication of laser treatment. In vascular tumors, involuted infantile hemangiomas constitute an excellent indication of pulsed-dye laser treatment. Controlled studies are necessary to evaluate and to compare the efficacy of each laser, in order to determine their optimal indications and optimal parameters for each machine. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

A frequency doubled pressure-tunable oscillator-amplifier dye laser system

NASA Technical Reports Server (NTRS)

Moriarty, A.; Heaps, W.; Davis, D. D.

1976-01-01

A tunable high-repetition-rate oscillator-amplifier dye-laser system is reported. The dye laser described was longitudinally pumped with the second harmonic of a Nd-YAG laser operating at 10 Hz. Using three Faraday-Perot etalons and pressure tuning, a maximum fundamental output power of the order of 6 MW with a corresponding spectral width of less than 0.003 nm at 564 nm was obtained. The fundamental at 564 nm was frequency doubled to give a maximum power level of 0.6 MW of second-harmonic output power with a spectral width less than 0.0015 nm at 282 nm. Frequency stability could be maintained to within approximately 15% of the line-width.

Pulsed dye laser treatment of rosacea using a novel 15 mm diameter treatment beam.

PubMed

Bernstein, Eric F; Schomacker, Kevin; Paranjape, Amit; Jones, Christopher J

2018-04-10

The pulsed-dye laser has been used to treat facial redness and rosacea for decades. Recent advances in dye laser technology enable 50% higher output energies supporting 50% larger treatment areas, and beam-diameters up to 15 mm with clinically-relevant fluences. In this study, we investigate this novel pulsed-dye laser using a 15 mm diameter beam for treatment of rosacea. Twenty subjects with erythemato-telangiectatic rosacea were enrolled in the study. A total of 4 monthly treatments were administered, first treating linear vessels with a 3 × 10 mm elliptical beam, then diffuse redness with a 15-mm diameter circular beam. Blinded assessment of digital, cross-polarized photographs taken 2 months following the last treatment was performed using an 11-point clearance scale. Nineteen subjects completed the study. Blinded reviewers correctly identified baseline photos in 55 out of the total of 57 images (96.5%). The blinded reviewers scored 17 of the 19 subjects with an improvement greater than 40%, and 11 of the 19 subjects greater than 50%. The average improvement was 53.9%. Side effects were limited to mild edema, mild to moderate erythema, and mild to moderate bruising. This study demonstrates that a newly designed pulsed-dye laser having a novel 15-mm diameter treatment beam improves the appearance of rosacea with a favorable safety profile. Lasers Surg. Med. 9999:1-5, 2018. © 2018 The Authors. Lasers in Surgery and Medicine Published by Wiley Periodicals, Inc. © 2018 The Authors. Lasers in Surgery and Medicine Published by Wiley Periodicals, Inc.

High conversion efficiency distributed feedback laser from a dye-doped holographic transmission grating

NASA Astrophysics Data System (ADS)

Liu, Lijuan; Zhang, Guiyang; Kong, Xiaobo; Liu, Yonggang; Xuan, Li

2018-01-01

A high conversion efficiency distributed feedback (DFB) laser from a dye-doped holographic polymer dispersed liquid crystal (HPDLC) transmission grating structure was reported. The alignment polyimide (PI) films were used to control the orientation of the phase separated liquid crystals (LCs) to increase the refractive index difference between the LC and the polymer, so it can provide better light feedback. The lasing wavelength located at 645.8 nm near the maximum of the amplified spontaneous emission (ASE) spectrum with the lowest threshold 0.97 μ J/pulse and the highest conversion efficiency 1.6% was obtained. The laser performance under electric field were also investigated and illustrated. The simple configuration, one-step fabrication organic dye laser shows the potential to realize ultra-low cost plastic lasers.

Design and application of fiber optic evanescent wave biosensor

NASA Astrophysics Data System (ADS)

Huang, Huijie; Zhai, Junhui; Zhao, Yongkai; Yang, Ruifu; Ren, Bingqiang; Cheng, Zhaogu; Du, Longlong; Lu, Dunwu

2003-12-01

A fiber-optic biosensor is developed based on the principle of evanescent wave while light propagates in optical fiber. The biosensor uses a red laser diode at 636.85 nm for exciting Cy5 fluorescent dye. Sensitivity limit of 0.01 nnmol/l is obtained from the detection of serial Cy5 solutions with various concentrations. In log-to-log plot, excellent linear response characteristic is seen in the Cy5 concentrations ranging from 0.01 nmlo/l to 100 nmol/l. And a good result of signal-to-noise ratio of 4.61 is obtained when the biosensor is used to measure Legionella pneumophila solution of 0.01 μmol/l. All the results are comparable with those that are obtained by a commercial biochip scanner GeneTAC 1000.

Adaptive optics in multiphoton microscopy: comparison of two, three and four photon fluorescence

PubMed Central

Sinefeld, David; Paudel, Hari P.; Ouzounov, Dimitre G.; Bifano, Thomas G.; Xu, Chris

2015-01-01

We demonstrate adaptive optics system based on nonlinear feedback from 3- and 4-photon fluorescence. The system is based on femtosecond pulses created by soliton self-frequency shift of a 1550-nm fiber-based femtosecond laser together with micro-electro-mechanical system (MEMS) phase spatial light modulator (SLM). We perturb the 1020-segment SLM using an orthogonal Walsh sequence basis set with a modified version of three-point phase shifting interferometry. We show the improvement after aberrations correction in 3-photon signal from fluorescent beads. In addition, we compare the improvement obtained in the same adaptive optical system for 2-, 3- and 4-photon fluorescence using dye pool. We show that signal improvement resulting from aberration correction grows exponentially as a function of the order of nonlinearity. PMID:26698772

Simple modification of basic dyes with bulky &symmetric WCAs for improving their solubilities in organic solvents without color change.

PubMed

Kim, Jeong Yun; Hwang, Tae Gyu; Woo, Sung Wun; Lee, Jae Moon; Namgoong, Jin Woong; Yuk, Sim Bum; Chung, Sei-Won; Kim, Jae Pil

2017-04-06

A simple and easy solubility enhancement of basic dyes was performed with bulky and symmetric weakly coordinating anions (WCAs). The WCAs decreased the ionic character of the dyes by broadening the partial charge distribution and causing a screening effect on the ionic bonding. This new modification with WCAs has advantages in that it has no influence on the optical properties of the dyes. The solubilities of unmodified and modified dyes were tested in several organic solvents. X-ray powder diffraction patterns of the dyes were measured. Color films were prepared with the dyes and their color loci were analyzed to evaluate the optical properties. By the modification with WCAs, commercial basic dyes showed sufficient solubilities for be applied to various applications while preserving their superior optical properties.

Near-ultraviolet laser diodes for brilliant ultraviolet fluorophore excitation.

PubMed

Telford, William G

2015-12-01

Although multiple lasers are now standard equipment on most modern flow cytometers, ultraviolet (UV) lasers (325-365 nm) remain an uncommon excitation source for cytometry. Nd:YVO4 frequency-tripled diode pumped solid-state lasers emitting at 355 nm are now the primary means of providing UV excitation on multilaser flow cytometers. Although a number of UV excited fluorochromes are available for flow cytometry, the cost of solid-state UV lasers remains prohibitively high, limiting their use to all but the most sophisticated multilaser instruments. The recent introduction of the brilliant ultraviolet (BUV) series of fluorochromes for cell surface marker detection and their importance in increasing the number of simultaneous parameters for high-dimensional analysis has increased the urgency of including UV sources in cytometer designs; however, these lasers remain expensive. Near-UV laser diodes (NUVLDs), a direct diode laser source emitting in the 370-380 nm range, have been previously validated for flow cytometric analysis of most UV-excited probes, including quantum nanocrystals, the Hoechst dyes, and 4',6-diamidino-2-phenylindole. However, they remain a little-used laser source for cytometry, despite their significantly lower cost. In this study, the ability of NUVLDs to excite the BUV dyes was assessed, along with their compatibility with simultaneous brilliant violet (BV) labeling. A NUVLD emitting at 375 nm was found to excite most of the available BUV dyes at least as well as a UV 355 nm source. This slightly longer wavelength did produce some unwanted excitation of BV dyes, but at sufficiently low levels to require minimal additional compensation. NUVLDs are compact, relatively inexpensive lasers that have higher power levels than the newest generation of small 355 nm lasers. They can, therefore, make a useful, cost-effective substitute for traditional UV lasers in multicolor analysis involving the BUV and BV dyes. Published 2015 Wiley Periodicals Inc. on behalf of ISAC.

Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects.

PubMed

Zehentbauer, Florian M; Moretto, Claudia; Stephen, Ryan; Thevar, Thangavel; Gilchrist, John R; Pokrajac, Dubravka; Richard, Katherine L; Kiefer, Johannes

2014-01-01

Rhodamine 6G (R6G), also known as Rhodamine 590, is one of the most frequently used dyes for application in dye lasers and as a fluorescence tracer, e.g., in the area of environmental hydraulics. Knowing the spectroscopic characteristics of the optical emission is key to obtaining high conversion efficiency and measurement accuracy, respectively. In this work, solvent and concentration effects are studied. A series of eight different organic solvents (methanol, ethanol, n-propanol, iso-propanol, n-butanol, n-pentanol, acetone, and dimethyl sulfoxide (DMSO)) are investigated at constant dye concentration. Relatively small changes of the fluorescence spectrum are observed for the different solvents; the highest fluorescence intensity is observed for methanol and lowest for DMSO. The shortest peak wavelength is found in methanol (568 nm) and the longest in DMSO (579 nm). Concentration effects in aqueous R6G solutions are studied over the full concentration range from the solubility limit to highly dilute states. Changing the dye concentration provides tunability between ∼550 nm in the dilute case and ∼620 nm at high concentration, at which point the fluorescence spectrum indicates the formation of R6G aggregates. Copyright © 2013 Elsevier B.V. All rights reserved.

Compact streak camera for the shock study of solids by using the high-pressure gas gun

NASA Astrophysics Data System (ADS)

Nagayama, Kunihito; Mori, Yasuhito

1993-01-01

For the precise observation of high-speed impact phenomena, a compact high-speed streak camera recording system has been developed. The system consists of a high-pressure gas gun, a streak camera, and a long-pulse dye laser. The gas gun installed in our laboratory has a muzzle of 40 mm in diameter, and a launch tube of 2 m long. Projectile velocity is measured by the laser beam cut method. The gun is capable of accelerating a 27 g projectile up to 500 m/s, if helium gas is used as a driver. The system has been designed on the principal idea that the precise optical measurement methods developed in other areas of research can be applied to the gun study. The streak camera is 300 mm in diameter, with a rectangular rotating mirror which is driven by an air turbine spindle. The attainable streak velocity is 3 mm/microsecond(s) . The size of the camera is rather small aiming at the portability and economy. Therefore, the streak velocity is relatively slower than the fast cameras, but it is possible to use low-sensitivity but high-resolution film as a recording medium. We have also constructed a pulsed dye laser of 25 - 30 microsecond(s) in duration. The laser can be used as a light source of observation. The advantage for the use of the laser will be multi-fold, i.e., good directivity, almost single frequency, and so on. The feasibility of the system has been demonstrated by performing several experiments.

Temperature-tunable lasing in negative dielectric chiral nematic liquid crystal

NASA Astrophysics Data System (ADS)

Wu, Ri-Na; Wu, Jie; Wu, Xiao-Jiao; Dai, Qin

2015-05-01

In this work, negative dielectric nematic liquid crystal SLC12V620-400, chiral dopant S811, and laser dye DCM are used to prepare dye-doped chiral nematic liquid crystal laser sample. In order to investigate temperature-tunable lasing in negative dielectric chiral nematic liquid crystal, we measure the transmission and lasing spectrum of this sample. The photonic band gap (PBG) is observed to red shift with its width reducing from 71.2 nm to 40.2 nm, and its short-wavelength band edge moves 55.3 nm while the long-wavelength band edge only moves 24.9 nm. The wavelength of output laser is found to red shift from 614.4 nm at 20 °C to 662.8 nm at 67 °C, which is very different from the previous experimental phenomena. The refractive indices, parallel and perpendicular to the director in chiral nematic liquid crystal have different dependencies on temperature. The experiment shows that the pitch of this chiral nematic liquid crystal increases with the increase of temperature. The decrease in the PBG width, different shifts of band edges, and the red shift of laser wavelength are the results of refractive indices change and pitch thermal elongation. Project supported by the National Natural Science Foundation of China (Grant No. 61378042), the Outstanding Young Scholars Growth Plans of Colleges and Universities in Liaoning Province, China (Grant No. LJQ2013022), the Science and Technology Research of Liaoning Province, China (Grant No. L2010465), the Open Funds of Liaoning Province Key Laboratory of Laser and Optical Information of Shenyang Ligong University, China.

Pulsed mononode dye laser developed for a geophysical application

NASA Technical Reports Server (NTRS)

Jegou, J. P.; Pain, T.; Megie, G.

1986-01-01

Following the extension of the lidar technique in the study of the atmosphere, the necessity of having a high power pulsed laser beam with a narrowed bandwidth and the possibility of selecting a particular wavelength within a certain spectral region arises. With the collaboration of others, a laser cavity using the multiwave Fizeau wedge (MWFW) was developed. Using the classical method of beam amplification with the aid of different stages, a new pulsed dye laser device was designed. The originality resides in the use of reflecting properties of the MFWF. Locally a plan wave coming with a particular angular incidence is reflected with a greater than unity coefficient; this is the consequence of the wedge angle which doubles the participation of every ray in the interferometric process. This dye laser operation and advantages are discussed. The feasibility of different geophysical applications envisageable with this laser is discussed.

Plastic scintillator with effective pulse shape discrimination for neutron and gamma detection

DOEpatents

Zaitseva, Natalia P.; Carman, M Leslie; Cherepy, Nerine; Glenn, Andrew M.; Hamel, Sebastien; Payne, Stephen A.; Rupert, Benjamin L.

2016-04-12

In one embodiment, a scintillator material includes a polymer matrix; and a primary dye in the polymer matrix, the primary dye being a fluorescent dye, the primary dye being present in an amount of 5 wt % or more; wherein the scintillator material exhibits an optical response signature for neutrons that is different than an optical response signature for gamma rays. In another embodiment, a scintillator material includes a polymer matrix; and a primary dye in the polymer matrix, the primary dye being a fluorescent dye, the primary dye being present in an amount greater than 10 wt %.

Intensity and absorbed-power distribution in a cylindrical solar-pumped dye laser

NASA Technical Reports Server (NTRS)

Williams, M. D.

1984-01-01

The internal intensity and absorbed-power distribution of a simplified hypothetical dye laser of cylindrical geometry is calculated. Total absorbed power is also calculated and compared with laboratory measurements of lasing-threshold energy deposition in a dye cell to determine the suitability of solar radiation as a pump source or, alternatively, what modifications, if any, are necessary to the hypothetical system for solar pumping.

Fabrication of Ocular Protective Devices against Laser Radiation and Ballistic Fragments for Evaluation in Military Scenarios

DTIC Science & Technology

1992-09-30

19 3.4.5 High Energy Laser ..... .............. .. 20 3.4.6 Solar ..................................... 21 3.4.7 Humidity...57 4.5.6 Solar ...................................... 57 4.5.7 Humidity... . .................. ...o.. ....57 4.5.8 Temperature...coated AO-ET dye 12. Effect of solar exposure on AO-ET dyeD 13. Spectral transmittance molded B-401 dye 14. Visible transmittance of typical two

Low-threshold ablation of enamel and dentin using Nd:YAG laser assisted with chromophore with different pulse shapes

NASA Astrophysics Data System (ADS)

Bonora, Stefano; Benazzato, Paolo; Stefani, Alessandro; Villoresi, Paolo

2004-05-01

Neodimium laser treatment has several drawbacks when used in the hard tissue cutting, because of the low absorption of the dental tissues at its wavelength. This investigation proved that the Nd:YAG radiation is a powerful ablation tool if it is used with the dye assisted method. Several in vitro tests on enamel and dentin were accomplished changing some laser parameters to have different pulse shapes and durations from 125μs up to 1.4ms. The importance of short time high power peaks, typical of crystal lasers, in the ablation process was investigated. The pulse shapes were analyzed by their intensity in space and time profiles. A first set of results found the optimum dye concentration be used in all the following tests. Furthermore the ablation threshold for this technique was found for each different pulse shapes and durations. A low energy ablation method was found to avoid temperature increase and surface cracks formation. In vitro temperature analysis was reported comparing the differences between no dye application laser treatment and with a dye spray applied. A strong reduction of the temperature increase was found in the dye assisted method. A discussion on the general findings and their possible clinical applications is presented.

Time-domain laser-induced fluorescence spectroscopy apparatus for clinical diagnostics

NASA Astrophysics Data System (ADS)

Fang, Qiyin; Papaioannou, Thanassis; Jo, Javier A.; Vaitha, Russel; Shastry, Kumar; Marcu, Laura

2004-01-01

We report the design and development of a compact optical fiber-based apparatus for in situ time-resolved laser-induced fluorescence spectroscopy (tr-LIFS) of biological systems. The apparatus is modular, optically robust, and compatible with the clinical environment. It incorporates a dual output imaging spectrograph, a gated multichannel plate photomultiplier (MCP-PMT), an intensified charge-coupled-device (ICCD) camera, and a fast digitizer. It can accommodate various types of light sources and optical fiber probes for selective excitation and remote light delivery/collection as required by different applications. The apparatus allows direct recording of the entire fluorescence decay with high sensitivity (nM range fluorescein dye concentration with signal-to-noise ratio of 46) and with four decades dynamic range. It is capable of resolving a broad range of fluorescence lifetimes from hundreds of picoseconds (as low as 300 ps) using the MCP-PMT coupled to the digitizer to milliseconds using the ICCD. The data acquisition and analysis process is fully automated, enabling fast recording of fluorescence intensity decay across the entire emission spectrum (0.8 s per wavelength or ˜40 s for a 200 nm wavelength range at 5 nm increments). The spectral and temporal responses of the apparatus were calibrated and its performance was validated using fluorescence lifetime standard dyes (Rhodamin B, 9-cyanoanthracene, and rose Bengal) and tissue endogenous fluorophores (elastin, collagen, nicotinamide adenine dinucleotide, and flavin adenine dinucleotide). Fluorescence decay lifetimes and emission spectra of all tested compounds measured with the current tr-LIFS apparatus were found in good agreement with the values reported in the literature. The design and performance of tr-LIFS apparatus have enabled in vivo studies of atherosclerotic plaques and brain tumors.

Laser-activated protein solder for peripheral nerve repair

NASA Astrophysics Data System (ADS)

Trickett, Rodney I.; Lauto, Antonio; Dawes, Judith M.; Owen, Earl R.

1995-05-01

A 100 micrometers core optical fiber-coupled 75 mW diode laser operating at a wavelength of 800 nm has been used in conjunction with a protein solder to stripe weld severed rat tibial nerves, reducing the long operating time required for microsurgical nerve repair. Welding is produced by selective laser denaturation of the albumin based solder which contains the dye indocyanine green. Operating time for laser soldering was 10 +/- 5 min. (n equals 20) compared to 23 +/- 9 min. (n equals 10) for microsuturing. The laser solder technique resulted in patent welds with a tensile strength of 15 +/- 5 g, while microsutured nerves had a tensile strength of 40 +/- 10 g. Histopathology of the laser soldered nerves, conducted immediately after surgery, displayed solder adhesion to the outer membrane with minimal damage to the inner axons of the nerves. An in vivo study is under way comparing laser solder repaired tibial nerves to conventional microsuture repair. At the time of submission 15 laser soldered nerves and 7 sutured nerves were characterized at 3 months and showed successful regeneration with compound muscle action potentials of 27 +/- 8 mV and 29 +/- 8 mW respectively. A faster, less damaging and long lasting laser based anastomotic technique is presented.

Inkjet-printed vertically emitting solid-state organic lasers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mhibik, Oussama; Chénais, Sébastien; Forget, Sébastien

In this paper, we show that Inkjet Printing can be successfully applied to external-cavity vertically emitting thin-film organic lasers and can be used to generate a diffraction-limited output beam with an output energy as high as 33.6 μJ with a slope efficiency S of 34%. Laser emission shows to be continuously tunable from 570 to 670 nm using an intracavity polymer-based Fabry-Perot etalon. High-optical quality films with several μm thicknesses are realized, thanks to ink-jet printing. We introduce a new optical material where EMD6415 commercial ink constitutes the optical host matrix and exhibits a refractive index of 1.5 and an absorption coefficientmore » of 0.66 cm{sup −1} at 550–680 nm. Standard laser dyes like Pyrromethene 597 and Rhodamine 640 are incorporated in solution to the EMD6415 ink. Such large size “printed pixels” of 50 mm{sup 2} present uniform and flat surfaces, with roughness measured as low as 1.5 nm in different locations of a 50 μm × 50 μm AFM scan. Finally, as the gain capsules fabricated by Inkjet printing are simple and do not incorporate any tuning or cavity element, they are simple to make, have a negligible fabrication cost, and can be used as fully disposable items. This work opens the way towards the fabrication of really low-cost tunable visible lasers with an affordable technology that has the potential to be widely disseminated.« less

Development of a passively Q-switched Nd:YAG microchip laser for use in the Satellite Laser Ranging 2000 project

NASA Astrophysics Data System (ADS)

Gompers, Samuel Leo

Presently, NASA is designing a replacement for its existing satellite laser ranging systems. These systems are used to measure Earth-satellite distances, tectonic plate movement, variations in rotational motion and other geodetic phenomena. Satellite Laser Ranging 2000 (SLR2000) is envisioned as a fully automated, sub- centimeter accuracy, eye-safe, low-cost replacement to the current SLR systems. It is expected to overcome present limitations by operating autonomously; being free of optical, chemical or electrical hazards; and having a greater average time between failures. Expected shot range precision is about one centimeter with normal point precision of better than three centimeters. This system will have twenty-four hour tracking coverage. SLR2000 specifications dictate operation at visible wavelengths with eye-safe energies on the order of one hundred microjoules and repetition rates on the order of two kilohertz. The optical subsystem of SLR2000 includes a passively Q- switched Nd:YAG microlaser. Passive Q-switching will be achieved using a saturable absorber and offers a number of advantages over the mode-locked lasers currently used in ranging stations: no need for long resonators with tight thermal control; no electro-optic switch required for single pulse selection; saturable absorbers precluding the use of carcinogenic dyes and solvents; and RF drive frequency electronics not tied to the resonator length of the laser cavity. The presented work describes the research and development of a prototype laser used to produce the energies, repetition rates and pulsewidths required for SLR2000. Optimization theories and models were applied to the laser design in order to accurately predict and assess performance characteristics of both gain medium and saturable absorber. Data were obtained which illustrated the affect of pump laser saturation and thermal lensing of the gain medium. Important laboratory skills and techniques were acquired in the design and construction of passively Q-switched microlasers.

Enhanced absorption in a reverse saturable absorbing dye blended with carbon nanotubes.

PubMed

Webster, Scott; Reyes-Reyes, Marisol; Williams, Richard; Carroll, David L

2008-12-01

Using nonlinear absorption at 532 nm in the nanosecond temporal regime, we have measured the low fluence nonlinear transmittance properties of the reverse saturable absorbing carbocyanine dye, 1,1',3,3,3',3'-hexamethylindotricarbocyanine iodide (HITCI), blended with well dispersed carbon nanotubes. The nonlinear optical properties of the blends are strongly dependent on the ratio of dye to nanotubes in solution. In the case where the nanotubes per dye molecule ratio is large, we see a distinctive enhancement in optical fluence limiting properties of the system, suggesting enhanced absorption of the excited states. However, when the nanotube to dye ratio decreases, the system's response is dominated by the behavior of the dye. We suggest that this can be understood as a two component system in which sensitized dye molecules associated with the nanotubes have an effectively different optical cross-section from the dye molecules far from the nanotubes. From classical antennae considerations, this is expected.

Dye-sensitized solar cells using laser processing techniques

NASA Astrophysics Data System (ADS)

Kim, Heungsoo; Pique, Alberto; Kushto, Gary P.; Auyeung, Raymond C. Y.; Lee, S. H.; Arnold, Craig B.; Kafafi, Zakia H.

2004-07-01

Laser processing techniques, such as laser direct-write (LDW) and laser sintering, have been used to deposit mesoporous nanocrystalline TiO2 (nc-TiO2) films for use in dye-sensitized solar cells. LDW enables the fabrication of conformal structures containing metals, ceramics, polymers and composites on rigid and flexible substrates without the use of masks or additional patterning techniques. The transferred material maintains a porous, high surface area structure that is ideally suited for dye-sensitized solar cells. In this experiment, a pulsed UV laser (355nm) is used to forward transfer a paste of commercial TiO2 nanopowder (P25) onto transparent conducting electrodes on flexible polyethyleneterephthalate (PET) and rigid glass substrates. For the cells based on flexible PET substrates, the transferred TiO2 layers were sintered using an in-situ laser to improve electron paths without damaging PET substrates. In this paper, we demonstrate the use of laser processing techniques to produce nc-TiO2 films (~10 μm thickness) on glass for use in dye-sensitized solar cells (Voc = 690 mV, Jsc = 8.7 mA/cm2, ff = 0.67, η = 4.0 % at 100 mW/cm2). This work was supported by the Office of Naval Research.

Assembly of Macrocycle Dye Derivatives into Particles for Fluorescence and Photoacoustic Applications.

PubMed

Lu, Hoang D; Lim, Tristan L; Javitt, Shoshana; Heinmiller, Andrew; Prud'homme, Robert K

2017-06-12

Optical imaging is a rapidly progressing medical technique that can benefit from the development of new and improved optical imaging agents suitable for use in vivo. However, the molecular rules detailing what optical agents can be processed and encapsulated into in vivo presentable forms are not known. We here present the screening of series of highly hydrophobic porphyrin, phthalocyanine, and naphthalocyanine dye macrocycles through a self-assembling Flash NanoPrecipitation process to form a series of water dispersible dye nanoparticles (NPs). Ten out of 19 tested dyes could be formed into poly(ethylene glycol) coated nanoparticles 60-150 nm in size, and these results shed insight on dye structural criteria that are required to permit dye assembly into NPs. Dye NPs display a diverse range of absorbance profiles with absorbance maxima within the NIR region, and have absorbance that can be tuned by varying dye choice or by doping bulking materials in the NP core. Particle properties such as dye core load and the compositions of co-core dopants were varied, and subsequent effects on photoacoustic and fluorescence signal intensities were measured. These results provide guidelines for designing NPs optimized for photoacoustic imaging and NPs optimized for fluorescence imaging. This work provides important details for dye NP engineering, and expands the optical imaging tools available for use.

Novel fluorescent contrast agents for optical imaging of in vivo tumors based on a receptor-targeted dye-peptide conjugate platform

NASA Astrophysics Data System (ADS)

Bugaj, Joseph E.; Achilefu, Samuel I.; Dorshow, Richard B.; Rajagopalan, Raghavan

2001-04-01

We have designed, synthesized, and evaluated the efficacy of novel dye-peptide conjugates that are receptor specific. Contrary to the traditional approach of conjugating dyes to large proteins and antibodies, we used small peptide-dye conjugates that target over-expressed receptors on tumors. Despite the fact that the peptide and the dye probe have similar molecular mass, our results demonstrate that the affinity of the peptide for its receptor and the dye fluorescence properties are both retained. The use of small peptides has several advantages over large biomolecules, including ease of synthesis of a variety of compounds for potential combinatorial screening of new targets, reproducibility of high purity compounds, diffusiveness to solid tumors, and the ability to incorporate a variety of functional groups that modify the pharmacokinetics of the peptide-dye conjugates. The efficacy of these new fluorescent optical contrast agents was evaluated in vivo in well-characterized rat tumor lines expressing somatostatin (sst2) and bombesin receptors. A simple continuous wave optical imaging system was employed. The resulting optical images clearly show that successful specific tumor targeting was achieved. Thus, we have demonstrated that small peptide- dye conjugates are effective as contrast agents for optical imaging of tumors.

Tracheal anastomosis using indocyanine green dye enhanced fibrinogen with a near-infrared diode laser

NASA Astrophysics Data System (ADS)

Auteri, Joseph S.; Jeevanandam, Valluvan; Oz, Mehmet C.; Libutti, Steven K.; Kirby, Thomas J.; Smith, Craig R.; Treat, Michael R.

1990-06-01

A major obstacle to lung transplantation and combined heart- lung transplantation is dehiscence of the tracheobronchial anastomosis. We explored the possibility of laser welded anastomoses in canine tracheas in vivo. Laser anastomoses were performed on three-quarter circumferential anterior tracheotomies. A continous wave diode laser (808 +1 nm) at a power density of 9.6 watts/cm was used. Human fibrinogen was mixed with indocyanine green dye (ICG, max absorbance 805 nm) and applied to the anastomosis site prior to laser exposure. Animals were sacrificed at 0, 21 and 28 days post-operatively. At sacrifice weld bursting pressures were measured by raising intratracheal pressure using forced ventilation via an endotracheal tube. Sutured and laser welded anastomoses had similar bursting pressures, and exhibited satisfactory histologic evidence of healing. However, compared to polypropylene sutured controls, the laser welded anastomoses exhibited less peritracheal inflammatory reaction and showed visibly smoother luminal surfaces at 21 and 28 days post- operatively. Tracheal anastomosis using ICG dye enhanced fibrinogen combined with the near-infrared diode laser is a promising extension of the technology of laser tissue fusion and deserves further study.

Polymer based plasmonic elements with dye molecules

NASA Astrophysics Data System (ADS)

Zhang, Douguo; Wang, Xiangxian; Chen, Yikai; Han, Lu; Wang, Pei; Ming, Hai

2012-11-01

Recently, dielectric loaded surface plasmons (SPs) elements are inducing highly interesting in the field of nanooptics, which are composed of dielectric nanostructures fabricated on a metallic thin film. This configuration will provide a route to novel integrated micro-optical devices and components combining photonics and electronics on the same chip. The advantages are easy fabrication, easy integration, and also the potential to realizing active plasmonic devices. In this talk, we will present our recent work in this field. Polymer (PMMA) nano-structures are fabricated on a silver film by the electron beam lithography (EBL) and laser interference lithography. These nano-structures are used to manipulate the behaviors of the SPs, such as converging, diverging, and guiding the propagation of SPs in subwavelength scale. Except for the pure PMMA nano-structures, dye materials (Rhodamine B, RhB) doped PMMA structures are also fabricated on the silver film. The RhB molecules will work as the active medium to excite the SPs or compensation the loss of SPs wave. The dye doped PMMA nanostructure provides a choice to realize active plasmonic elements, such as SPs Bragg gratings. On the other hand, the interaction between the fluorescence molecules and SPs will give rise to some new optical phenomena, such as directional fluorescence emission, anisotropic fluorescence emission. These polymer based plasmonic structures are investigated with a home-built leakage radiation microscopy (LRM).

Direct and comprehensive analysis of dyes based on integrated molecular and structural information via laser desorption laser postionization mass spectrometry.

PubMed

Liu, Rong; Yin, Zhibin; Leng, Yixin; Hang, Wei; Huang, Benli

2018-01-01

Laser desorption laser postionization time-of-flight mass spectrometry (LDPI-TOFMS) was employed for direct analysis and determination of typical basic dyes. It was also used for the analysis and comprehensive understanding of complex materials such as blue ballpoint pen inks. Simultaneous emergences of fragmental and molecular information largely simplify and facilitate unambiguous identification of dyes via variable energy of 266nm postionization laser. More specifically, by optimizing postionization laser energy with the same energy of desorption laser, the structurally significant results show definite differences in the fragmentation patterns, which offer opportunities for discrimination of isomeric species with identical molecular weight. Moreover, relatively high spectra resolution can be acquired without the expense of sensitivity. In contrast to laser desorption/ionization mass spectrometry (LDI-MS), LDPI-MS simultaneously offers valuable molecular information about dyes in traces, solvents and additives about inks, thereby offering direct determination and comprehensive understanding of blue ballpoint inks and giving a high level of confidence to discriminate the complicated evidentiary samples. In addition, direct analysis of the inks not only allows the avoidance of the tedious sample preparation processes, significantly shortening the overall analysis time and improving throughput, but allows minimized sample consumption which is important for rare and precious samples. Copyright © 2017 Elsevier B.V. All rights reserved.

LASERS, ACTIVE MEDIA: The aqueous-polyelectrolyte dye solution as an active laser medium

NASA Astrophysics Data System (ADS)

Akimov, A. I.; Saletskii, A. M.

2000-11-01

The spectral, luminescent, and lasing properties of aqueous solutions of a cationic dye rhodamine 6G with additions of anion polyelectrolytes — polyacrylic and polymethacrylic acids — are studied. It is found that the energy and spectral properties of lasing of these solutions depend on the ratio of concentrations of polyelectrolyte and molecules. It is also found that the lasing parameters of aqueous-polyelectrolyte dye solutions can be controlled by changing the structure of the molecular system. The variation in the structure of aqueous-polyelectrolyte dye solutions of rhodamine 6G resulted in an almost five-fold increase in the lasing efficiency compared to that in aqueous dye solutions.

Effect of DNA-CTMA complex on optical properties of LDS 821 dye

NASA Astrophysics Data System (ADS)

Udayan, Sony; Ramachandran, Vijesh Kavumoottil; Sebastian, Mathew; Chandran, Pradeep; Nampoori, Vadakkedath Parameswaran Narayanan; Thomas, Sheenu

2017-07-01

We have investigated the fluorescence behavior of LDS 821 dye (Styryl 9 M) with deoxyribonucleic acid attached with cetyltrimethyl-ammonium (DNA-CTMA). Optical absorption studies confirm the intercalation of the dye molecules with DNA-CTMA. Fluorescence studies show an enhancement of fluorescence intensity of dye with DNA-CTMA, which suggest the reduction of TICT states of the dye molecule. The FWHM of the fluorescence spectrum increases from 95 nm to 161 nm indicating the formation of new energy levels when DNA-CTMA forms a complex with LDS 821 dye. Fluorescence lifetime measurements shows that lifetime of LDS 821 varies from 507ps to 953 ps with the addition of DNA-CTMA, which also confirms the deactivation of TICT states of dye molecule. Results show that the incorporation of DNA-CTMA with LDS 821 dye improves the optical characteristics of LDS 821 dye and therefore, can be used as a good fluorescence probe for DNA visualization as well as in lasing applications.

Theoretical studies on a new pattern of laser-driven systems: towards elucidation of direct photo-injection in dye-sensitized solar cells

NASA Astrophysics Data System (ADS)

Mishima, Kenji; Yamashita, Koichi

2011-03-01

We theoretically and numerically investigated a new type of analytically solvable laser-driven systems inspired by electron-injection dynamics in dye-sensitized solar cells. The simple analytical expressions were found to be useful for understanding the difference between dye excitation and direct photo-injection occurring between dye molecule and semiconductor nanoparticles. More importantly, we propose a method for discriminating experimentally dye excitation and direct photo-injection by using time-dependent fluorescence. We found that dye excitation shows no significant quantum beat whereas the direct photo-injection shows a significant quantum beat. This work was supported by Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST) ``Development of Organic Photovoltaics toward a Low-Carbon Society,'' Cabinet Office, Japan.

Two-dimensional microsphere quasi-crystal: fabrication and properties

NASA Astrophysics Data System (ADS)

Noginova, Natalia E.; Venkateswarlu, Putcha; Kukhtarev, Nickolai V.; Sarkisov, Sergey S.; Noginov, Mikhail A.; Caulfield, H. John; Curley, Michael J.

1996-11-01

2D quasi-crystals were fabricated from polystyrene microspheres and characterized for their structural, diffraction, and non-linear optics properties. The quasi- crystals were produced with the method based on Langmuir- Blodgett thin film technique. Illuminating the crystal with the laser beam, we observed the diffraction pattern in the direction of the beam propagation and in the direction of the back scattering, similar to the x-ray Laue pattern observed in regular crystals with hexagonal structure. The absorption spectrum of the quasi-crystal demonstrated two series of regular maxima and minima, with the spacing inversely proportional to the microspheres diameter. Illumination of the dye-doped microspheres crystal with Q- switched radiation of Nd:YAG laser showed the enhancement of non-linear properties, in particular, second harmonic generation.

A mercury arc lamp-based multi-color confocal real time imaging system for cellular structure and function.

PubMed

Saito, Kenta; Kobayashi, Kentaro; Tani, Tomomi; Nagai, Takeharu

2008-01-01

Multi-point scanning confocal microscopy using a Nipkow disk enables the acquisition of fluorescent images with high spatial and temporal resolutions. Like other single-point scanning confocal systems that use Galvano meter mirrors, a commercially available Nipkow spinning disk confocal unit, Yokogawa CSU10, requires lasers as the excitation light source. The choice of fluorescent dyes is strongly restricted, however, because only a limited number of laser lines can be introduced into a single confocal system. To overcome this problem, we developed an illumination system in which light from a mercury arc lamp is scrambled to make homogeneous light by passing it through a multi-mode optical fiber. This illumination system provides incoherent light with continuous wavelengths, enabling the observation of a wide range of fluorophores. Using this optical system, we demonstrate both the high-speed imaging (up to 100 Hz) of intracellular Ca(2+) propagation, and the multi-color imaging of Ca(2+) and PKC-gamma dynamics in living cells.

Utilization of a new optical sensor unit to monitor the electrochemical elimination of selected dyes in water

NASA Astrophysics Data System (ADS)

Valica, M.; Černá, T.; Hostin, S.

2017-10-01

This paper presents results obtained by developed optical sensor, which consist from multi-wavelength LED light source and two photodetectors capable of measuring the change in optical signal along two different optical paths (absorbance and reflectance measurements). Arduino microcomputer was used for light source management and optical signal data measuring and recording. Analytical validation of developed optical sensor is presented in this paper. The performance of the system has been tested with varying water solution of dyes (malachite green, methyl orange, trypan red). These results show strong correlations between the optical signal response and colour change from the dyes. Sensor was used for continual in-situ monitoring of electrochemical elimination of selected dyes (current density 15.7 mA cm-2, electrolyte volume 4 L and NaCl concentration 2 g L-1). Maximum decolorization level varies with each dye. For malachite green was obtain 92,7 % decolorization (25 min); methyl orange 90,8% (8,5 min) and trypan red 84,7% decolorization after 33 min of electrochemical treatment.

Laser performance of Coumarin 540A dye molecules in polymeric host media with different viscosities: From liquid solution to solid polymer matrix

DOE Office of Scientific and Technical Information (OSTI.GOV)

Costela, A.; Garcia-Moreno, I.; Barroso, J.

1998-01-01

Photophysical parameters and lasing properties of Coumarin 540A dye molecules are studied in solutions of increasing viscosity, from liquid solutions in 1,4-dioxane to solid solutions in poly(methyl methacrylate). The fluorescence quantum yield and lasing efficiencies decrease as the viscosity of the solution increases, reflecting the strong influence of the rigidity of the medium on the radiative processes. The photodegradation mechanisms acting on the fluorophores are analyzed by following the dependence of laser induced fluorescence and laser output on the number of pump laser pulses. The fluorescence redistribution after pattern photobleaching technique is used, and Fick{close_quote}s second law is applied tomore » study the diffusion of dye molecules in the highly viscous polymer solutions. The diffusion coefficients of the dye molecules as a function of the increased viscosity of the medium are determined. {copyright} {ital 1998 American Institute of Physics.}« less

Development of new near-infrared and leuco-dye optical systems for forensic and crime fighting applications

NASA Astrophysics Data System (ADS)

Patonay, Gabor; Strekowski, Lucjan; Salon, Jozef; Medou-Ovono, Martial; Krutak, James J.; Leggitt, Jeffrey; Seubert, Heather; Craig, Rhonda

2004-12-01

New chemistry for leuco fluorescin and leuco rhodamine for latent bloodstain and fingerprint detection has been developed in our laboratories. The use of these leuco dyes results in excellent contrast for several hours. The FBI's Evidence Response Team and DNA I unit collaborated with Georgia State University to validate the new fluorescin chemistry for use in the field. In addition, several new NIR dyes have been developed in our laboratories that can be used to detect different chemical residues, e.g., pepper spray, latent fingerprint, latent blood, metal ions, or other trace evidence during crime scene investigations. Proof of principle experiments showed that NIR dyes reacting with such residues can be activated with appropriately filtered semiconductor lasers and LEDs to emit NIR fluorescence that can be observed using optimally filtered night vision intensifiers or pocket scopes, digital cameras, CCD and CMOS cameras, or other NIR detection systems. The main advantage of NIR detection is that the color of the background has very little influence on detection and that there are very few materials that would interfere by exhibiting NIR fluorescence. The use of pocket scopes permits sensitive and convenient detection. Once the residues are located, digital images of the fluorescence can be recorded and samples obtained for further analyses. NIR dyes do not interfere with subsequent follow-up or confirmation methods such as DNA or LC/MS analysis. Near-infrared absorbing dyes will be summarized along with detection mechanisms.

Comparison of different laser systems in the treatment of hypertrophic and atrophic scars and keloids

NASA Astrophysics Data System (ADS)

Scharschmidt, D.; Algermissen, Bernd; Willms-Jones, J.-C.; Philipp, Carsten M.; Berlien, Hans-Peter

1997-12-01

Different laser systems and techniques are used for the treatment of hypertrophic scars, keloids and acne scars. Significant criteria in selecting a suitable laser system are the scar's vascularization, age and diameter. Flashlamp- pumped dye-lasers, CO2-lasers with scanner, Argon and Nd:YAG-lasers are used. Telangiectatic scars respond well to argon lasers, erythematous scars and keloids to dye-laser treatment. Using interstitial Nd:YAG-laser vaporization, scars with a cross-section over 1 cm can generally be reduced. For the treatment of atrophic and acne scars good cosmetic results are achieved with a CO2-laser/scanner system, which allows a precise ablation of the upper dermis with low risk of side-effects.

Flow and thermal characteristics of high Reynolds number (2800-17,000) dye cell: simulation and experiment.

PubMed

Mishra, G K; Kumar, Abhay; Prakash, O; Biswal, R; Dixit, S K; Nakhe, S V

2015-04-10

This paper presents computational and experimental studies on wavelength/frequency fluctuation characteristics of a high pulse repetition rate (18 kHz) dye laser pumped by a frequency-doubled Nd:YAG laser (532 nm). The temperature gradient in the dye solution is found to be responsible for wavelength fluctuations of the dye laser at low flow rates (2800

Intracavity absorption with a continuous wave dye laser - Quantification for a narrowband absorber

NASA Technical Reports Server (NTRS)

Brobst, William D.; Allen, John E., Jr.

1987-01-01

An experimental investigation of the dependence of intracavity absorption on factors including transition strength, concentration, absorber path length, and pump power is presented for a CW dye laser with a narrow-band absorber (NO2). A Beer-Lambert type relationship is found over a small but useful range of these parameters. Quantitative measurement of intracavity absorption from the dye laser spectral profiles showed enhancements up to 12,000 (for pump powers near lasing threshold) when compared to extracavity measurements. The definition of an intracavity absorption coefficient allowed the determination of accurate transition strength ratios, demonstrating the reliability of the method.

Experimental study of cavity configurations for dye lasers pumped by a copper vapor laser

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tang Chaunshun; Sun Wei

1988-04-01

Four cavity configurations are considered for dye lasers pumped transversely by a CuBr laser at high pulse repetition frequencies. Their operating characteristics are compared. Optimum performance is found for a double-prism expander cavity equipped with a Littrow mounted grating. A single longitudinal mode lasing in the 598--640 nm range was achieved with a linewidth of 0.0012 nm and a conversion of efficiency of 7.5%, respectively. The amplified spontaneous emission was 1.5%.

Bichromatic emission in a ring dye laser

NASA Technical Reports Server (NTRS)

Lawandy, N. M.; Sohrab Afzal, R.; Rabinovich, W. S.

1987-01-01

An experimental study of a high-Q Rhodamine 6G ring dye laser has been performed, and bichromatic emission (BE) with wavelength spacings as large as 110 A when the laser operated bidirectionally has been measured. The BE vanished at all excitations when the laser was forced into unidirectional operation using a Faraday isolator. However, when a weak reflected beam was allowed to make a single pass in the direction opposite to that allowed by the Faraday device, BE is recovered at the higher pump powers.

Holographic high-resolution endoscopic image recording

NASA Astrophysics Data System (ADS)

Bjelkhagen, Hans I.

1991-03-01

Endoscopic holography or endoholography combines the features of endoscopy and holography. The purpose of endoholographic imaging is to provide the physician with a unique means of extending diagnosis by providing a life-like record of tissue. Endoholographic recording will provide means for microscopic examination of tissue and in some cases may obviate the need to excise specimens for biopsy. In this method holograms which have the unique properties of three-dimensionality large focal depth and high resolution are made with a newly designed endoscope. The endoscope uses a single-mode optical fiber for illumination and single-beam reflection holograms are recorded in close contact with the tissue at the distal end of the endoscope. The holograms are viewed under a microscope. By using the proper combinations of dyes for staining specific tissue types with various wavelengths of laser illumination increased contrast on the cellular level can be obtained. Using dyes such as rose bengal in combination with the 514. 5 nm line of an argon ion laser and trypan blue or methylene blue with the 647. 1 nm line of a krypton ion laser holograms of the stained colon of a dog showed the architecture of the colon''s columnar epithelial cells. It is hoped through chronological study using this method in-vivo an increased understanding of the etiology and pathology of diseases such as Crohn''s diseases colitis proctitis and several different forms of cancer will help

Tissue characterization by time-resolved fluorescence spectroscopy of endogenous and exogenous fluorochromes: apparatus design and preliminary results

NASA Astrophysics Data System (ADS)

Glanzmann, Thomas M.; Ballini, Jean-Pierre; Jichlinski, Patrice; van den Bergh, Hubert; Wagnieres, Georges A.

1996-12-01

The biomedical use of an optical fiber-based spectro- temporal fluorometer that can endoscopically record the fluorescence decay of an entire spectrum without scanning is presented. The detector consists of a streak camera coupled to a spectrograph. A mode-locked argon ion pumped dye laser or a nitrogen laser-pumped dye laser are used as pulsed excitation light sources. We measured the fluorescence decays of endogenous fluorophores and of ALA-induced- protoporphyrin IX(PPIX) in an excised human bladder with a carcinoma in situ (CIS). Each autofluorescence decay can be decomposed in at least three exponential components for all tissue samples investigated if the excitation is at 425 nm. The decays of the autofluorescence of all normal sites of the human bladder are similar and they differ significantly from the decays measured on the CIS and the necrotic tissue. The fluorescence of the ALA-induced PPIX in the bladder is monoexponential with a lifetime of 15 (plus or minus 1) ns and this fluorescence lifetime does not change significantly between the normal urothelium and the CIS. A photoproduct of ALA-PPIX with a fluorescence maximum at 670 nm and a lifetime of 8 (plus or minus 1) ns was observed. The measurement of the decay of the autofluorescence allowed to correctly identify a normal tissue site that was classified as abnormal by the measurement of the ALA-PPIX fluorescence intensity.

Photophysics of Laser Dye-Doped Polymer Membranes for Laser-Induced Fluorescence Photogrammetry

NASA Technical Reports Server (NTRS)

Dorrington, Adrian A.; Jones, Thomas W.; Danehy, Paul M.

2004-01-01

Laser-induced fluorescence target generation in dye-doped polymer films has recently been introduced as a promising alternative to more traditional photogrammetric targeting techniques for surface profiling of highly transparent or reflective membrane structures. We investigate the photophysics of these dye-doped polymers to help determine their long-term durability and suitability for laser-induced fluorescence photogrammetric targeting. These investigations included experimental analysis of the fluorescence emission pattern, spectral content, temporal lifetime, linearity, and half-life. Results are presented that reveal an emission pattern wider than normal Lambertian diffuse surface scatter, a fluorescence time constant of 6.6 ns, a pump saturation level of approximately 20 micro J/mm(exp 2), and a useful lifetime of more than 300,000 measurements. Furthermore, two demonstrations of photogrammetric measurements by laser-induced fluorescence targeting are presented, showing agreement between photogrammetric and physically measured dimensions within the measurement scatter of 100 micron.

Characterization of Fluorescent Polystyrene Microspheres for Advanced Flow Diagnostics

NASA Technical Reports Server (NTRS)

Maisto, Pietro M. F.; Lowe, K. Todd; Byun, Guibo; Simpson, Roger; Vercamp, Max; Danley, Jason E.; Koh, Brian; Tiemsin, Pacita; Danehy, Paul M.; Wohl, Christopher J.

2013-01-01

Fluorescent dye-doped polystyrene latex microspheres (PSLs) are being developed for velocimetry and scalar measurements in variable property flows. Two organic dyes, Rhodamine B (RhB) and dichlorofluorescence (DCF), are examined to assess laser-induced fluorescence (LIF) properties for flow imaging applications and single-shot temperature measurements. A major interest in the current research is the application of safe dyes, thus DCF is of particular interest, while RhB is used as a benchmark. Success is demonstrated for single-point laser Doppler velocimetry (LDV) and also imaging fluorescence, excited via a continuous wave 2 W laser beam, for exposures down to 10 ms. In contrast, when exciting with a pulsed Nd:YAG laser at 200 mJ/pulse, no fluorescence was detected, even when integrating tens of pulses. We show that this is due to saturation of the LIF signal at relatively low excitation intensities, 4-5 orders of magnitude lower than the pulsed laser intensity. A two-band LIF technique is applied in a heated jet, indicating that the technique effectively removes interfering inputs such as particle diameter variation. Temperature measurement uncertainties are estimated based upon the variance measured for the two-band LIF intensity ratio and the achievable dye temperature sensitivity, indicating that particles developed to date may provide about +/-12.5 C precision, while future improvements in dye temperature sensitivity and signal quality may enable single-shot temperature measurements with sub-degree precision.

An imaging system for PLIF/Mie measurements for a combusting flow

NASA Technical Reports Server (NTRS)

Wey, C. C.; Ghorashi, B.; Marek, C. J.; Wey, C.

1990-01-01

The equipment required to establish an imaging system can be divided into four parts: (1) the light source and beam shaping optics; (2) camera and recording; (3) image acquisition and processing; and (4) computer and output systems. A pulsed, Nd:YAG-pummped, frequency-doubled dye laser which can freeze motion in the flowfield is used for an illumination source. A set of lenses is used to form the laser beam into a sheet. The induced fluorescence is collected by an UV-enhanced lens and passes through an UV-enhanced microchannel plate intensifier which is optically coupled to a gated solid state CCD camera. The output of the camera is simultaneously displayed on a monitor and recorded on either a laser videodisc set of a Super VHS VCR. This videodisc set is controlled by a minicomputer via a connection to the RS-232C interface terminals. The imaging system is connected to the host computer by a bus repeater and can be multiplexed between four video input sources. Sample images from a planar shear layer experiment are presented to show the processing capability of the imaging system with the host computer.

The pulsed dye laser versus the Q-switched Nd:YAG laser in laser-induced shock-wave lithotripsy.

PubMed

Thomas, S; Pensel, J; Engelhardt, R; Meyer, W; Hofstetter, A G

1988-01-01

To date, there are two fairly well-established alternatives for laser-induced shock-wave lithotripsy in clinical practice. The Q-switched Nd:YAG laser is distinguished by the high-stone selectivity of its coupler systems. The necessity of a coupler system and its fairly small conversion rate of light energy into mechanical energy present serious drawbacks. Furthermore, the minimal outer diameter of the transmission system is 1.8 mm. The pulsed-dye laser can be used with a highly flexible and uncomplicated 200-micron fiber. However, the laser system itself is more complicated than the Q-switched Nd:YAG laser and requires a great deal of maintenance. Biological evaluation of damage caused by direct irradiation shows that both laser systems produce minor damage of different degrees. YAG laser lithotripsy with the optomechanical coupler was assessed in 31 patients with ureteral calculi. The instability and limited effectiveness of the fiber application system necessitated auxiliary lithotripsy methods in 14 cases. Dye-laser lithotripsy is currently being tested in clinical application. Further development, such as systems for blind application or electronic feedback mechanisms to limit adverse tissue effects, have yet to be optimized. Nevertheless, laser-induced shock-wave lithotripsy has the potential to become a standard procedure in the endourologic management of stone disease.

Monolithic dye laser amplifier

DOEpatents

Kuklo, T.C.

1993-03-30

A fluid dye laser amplifier for amplifying a dye beam by pump beams has a channel structure defining a channel through which a laseable fluid flows and the dye and pump beams pass transversely to one another through a lasing region. The channel structure is formed with two pairs of mutually spaced-apart and mutually confronting glass windows, which are interlocked and make surface-contacts with one another and surround the lasing region. One of the glass window pairs passes the dye beam and the other passes the pump beams therethrough and through the lasing region. Where these glass window pieces make surface-contacts, glue is used to join the pieces together to form a monolithic structure so as to prevent the dye in the fluid passing through the channel from entering the space between the mutually contacting glass window pieces.

Monolithic dye laser amplifier

DOEpatents

Kuklo, Thomas C.

1993-01-01

A fluid dye laser amplifier for amplifying a dye beam by pump beams has a channel structure defining a channel through which a laseable fluid flows and the dye and pump beams pass transversely to one another through a lasing region. The channel structure is formed with two pairs of mutually spaced-apart and mutually confronting glass windows, which are interlocked and make surface-contacts with one another and surround the lasing region. One of the glass window pairs passes the dye beam and the other passes the pump beams therethrough and through the lasing region. Where these glass window pieces make surface-contacts, glue is used to join the pieces together to form a monolithic structure so as to prevent the dye in the fluid passing through the channel from entering the space between the mutually contacting glass window pieces.

Transcutaneous laser treatment of leg veins.

PubMed

Meesters, Arne A; Pitassi, Luiza H U; Campos, Valeria; Wolkerstorfer, Albert; Dierickx, Christine C

2014-03-01

Leg telangiectasias and reticular veins are a common complaint affecting more than 80% of the population to some extent. To date, the gold standard remains sclerotherapy for most patients. However, there may be some specific situations, where sclerotherapy is contraindicated such as needle phobia, allergy to certain sclerosing agents, and the presence of vessels smaller than the diameter of a 30-gauge needle (including telangiectatic matting). In these cases, transcutaneous laser therapy is a valuable alternative. Currently, different laser modalities have been proposed for the management of leg veins. The aim of this article is to present an overview of the basic principles of transcutaneous laser therapy of leg veins and to review the existing literature on this subject, including the most recent developments. The 532-nm potassium titanyl phosphate (KTP) laser, the 585-600-nm pulsed dye laser, the 755-nm alexandrite laser, various 800-983-nm diode lasers, and the 1,064-nm neodymium yttrium-aluminum-garnet (Nd:YAG) laser and various intense pulsed light sources have been investigated for this indication. The KTP and pulsed dye laser are an effective treatment option for small vessels (<1 mm). The side effect profile is usually favorable to that of longer wavelength modalities. For larger veins, the use of a longer wavelength is required. According to the scarce evidence available, the Nd:YAG laser produces better clinical results than the alexandrite and diode laser. Penetration depth is high, whereas absorption by melanin is low, making the Nd:YAG laser suitable for the treatment of larger and deeply located veins and for the treatment of patients with dark skin types. Clinical outcome of Nd:YAG laser therapy approximates that of sclerotherapy, although the latter is associated with less pain. New developments include (1) the use of a nonuniform pulse sequence or a dual-wavelength modality, inducing methemoglobin formation and enhancing the optical absorption properties of the target structure, (2) pulse stacking and multiple pass laser treatment, (3) combination of laser therapy with sclerotherapy or radiofrequency, and (4) indocyanin green enhanced laser therapy. Future studies will have to confirm the role of these developments in the treatment of leg veins. The literature still lacks double-blind controlled clinical trials comparing the different laser modalities with each other and with sclerotherapy. Such trials should be the focus of future research.

Photophysical parameters and laser performance of 3-(4‧-dimethylaminophenyl)-1-(2-furanyl)prop-2-en-1-one (DMAFP): A new laser dye

NASA Astrophysics Data System (ADS)

El-Daly, S. A.; Gaber, M.; El-Sayed, Y. S.

2009-09-01

The spectral properties such as singlet absorption, molar absorptivity, emission spectra, fluorescence quantum yield and excited state lifetime of 3-(4'-dimethylaminophenyl)-1-(2-furanyl)prop-2-en-1-one (DMAFP) have been determined in different solvents. DMAFP dye exhibits a large red shift in both electronic absorption and emission spectra as the solvent polarity increases, indicating a large change in the dipole moment of molecules upon excitation. A crystalline solid of DMAFP gives an excimer like emission at 566 nm due to the excitation of molecular aggregates. This is expected from the idealized crystal structure of the dye that belongs to the B-type class of Steven's classification. The ground and excited state protonation constants of DMAFP are calculated and amounted to 1.71 and 8.3, respectively. DMAFP acts as a good laser dye upon pumping with nitrogen laser ( λex=337.1 nm) in chloroform, methylene chloride and dioxane and gives laser emission in the range 460-590 nm. The laser parameters such as the tuning range, gain coefficient ( α), emission cross section ( σ e) and half-life energy ( E1/2) are calculated. The photoreactivity and net photochemical quantum yield of DMAFP in chloromethane solvents are also studied.

Combined pulsed dye and CO2 lasers in the treatment of angiolymphoid hyperplasia with eosinophilia.

PubMed

Sagi, Lior; Halachmi, Shlomit; Levi, Assi; Amitai, Dan Ben; Enk, Claes D; Lapidoth, Moshe

2016-08-01

Angiolymphoid hyperplasia with eosinophilia (ALHE) is an uncommon dermatosis of unknown etiology that manifests as characteristic red nodules and papules with a predilection for the scalp and periauricular region. Treatment is required for both esthetic and functional reasons, as lesions may ulcerate and bleed. Many treatment approaches have been reported, including excision, systemic medical approaches, topical or intralesional therapies, and non-invasive modalities including cryotherapy, electrosurgery, and laser. Treatments have exhibited variable efficacy, and the recurrence rate is 100 %. We report the combination of pulsed dye laser and CO2 laser in the treatment of ALHE in 14 patients. All patients exhibited clinical response after a mean of 2.4 ± 0.4 treatment sessions. The clinical efficacy of the combined treatment, together with its well-tolerated nature, render the use of pulsed dye laser in combination with CO2 laser, a viable treatment for debulking ALHE lesions. Ongoing maintenance treatments are needed to due to the high degree of relapse.

Superradiant laser emission from rhodamine 6G and rhodamine B using a coaxial flashpump.

NASA Technical Reports Server (NTRS)

Mumola, P. B.

1972-01-01

Superradiant laser emission has been observed from ethanol solutions of rhodamine 6G and rhodamine B using a coaxial flashlamp pump. The dependence of input threshold energy on dye concentration is examined. The possibility of exciting other dyes to superradiance is discussed.

Convenient Microscale Synthesis of a Coumarin Laser Dye Analog

ERIC Educational Resources Information Center

Aktoudianakis, Evangelos; Dicks, Andrew P.

2006-01-01

Coumarin (2H-1-benzopyran-2-one) and its derivatives constitute a fascinating class of organic substances that are utilized industrially in areas such as cosmetics, food preservatives, insecticides and fluorescent laser dyes. The product can be synthesized, purified, and characterized within two hours with benefits of microscale reactivity being…

Robust remote-pumping sodium laser for advanced LIDAR and guide star applications

NASA Astrophysics Data System (ADS)

Ernstberger, Bernhard; Enderlein, Martin; Friedenauer, Axel; Schwerdt, Robin; Wei, Daoping; Karpov, Vladimir; Leisching, Patrick; Clements, Wallace R. L.; Kaenders, Wilhelm G.

2015-10-01

The performance of large ground-based optical telescopes is limited due to wavefront distortions induced by atmospheric turbulence. Adaptive optics systems using natural guide stars with sufficient brightness provide a practical way for correcting the wavefront errors by means of deformable mirrors. Unfortunately, the sky coverage of bright stars is poor and therefore the concept of laser guide stars was invented, creating an artificial star by exciting resonance fluorescence from the mesospheric sodium layer about 90 km above the earth's surface. Until now, mainly dye lasers or sumfrequency mixing of solid state lasers were used to generate laser guide stars. However, these kinds of lasers require a stationary laser clean room for operation and are extremely demanding in maintenance. Under a development contract with the European Southern Observatory (ESO) and W. M. Keck Observatory (WMKO), TOPTICA Photonics AG and its partner MPB Communications have finalized the development of a next-generation sodium guide star laser system which is available now as a commercial off-the-shelf product. The laser is based on a narrow-band diode laser, Raman fiber amplifier (RFA) technology and resonant second-harmonic generation (SHG), thus highly reliable and simple to operate and maintain. It emits > 22 W of narrow-linewidth (≈ 5 MHz) continuous-wave radiation at sodium resonance and includes a re-pumping scheme for boosting sodium return flux. Due to the SHG resonator acting as spatial mode filter and polarizer, the output is diffraction-limited with RMS wavefront error < λ/25. Apart from this unique optical design, a major effort has been dedicated to integrating all optical components into a ruggedized system, providing a maximum of convenience and reliability for telescope operators. The new remote-pumping architecture allows for a large spatial separation between the main part of the laser and the compact laser head. Together with a cooling-water flow of less than 5 l/min and an overall power consumption of < 700 W, the system offers a maximum of flexibility with minimal infrastructure demands on site. Each system is built in a modular way, based on the concept of line-replaceable units (LRU). A comprehensive system software, as well as an intuitive service GUI, allow for remote control and error tracking down to at least the LRU level. In case of a failure, any LRU can be easily replaced. With these fiber-based guide star lasers, TOPTICA for the first time offers a fully engineered, off-the-shelf guide star laser system for groundbased optical telescopes providing convenient, turn-key operation in remote and harsh locations. Reliability and flexibility will be beneficial in particular for advanced satellite and space debris tracking as well as LIDAR applications.

Cyanine dyes as optical contrast agents for ophthalmological surgery.

PubMed

Langhals, Heinz; Varja, Ana; Laubichler, Peter; Kernt, Marcus; Eibl, Kirsten; Haritoglou, Christos

2011-06-09

Cyanine dyes were prepared as optical contrast media for supporting the surgery of the lamina limitans interna (LLI) of the retina and other structures of the human eye. Their absorption spectra were adapted both to the spectral sensitivity of the human eye and to standard illumination. The contrast could be further amplified by the application of the strong fluorescence of the dyes used. The binding of the dyes to various surfaces was studied. No toxic effects could be detected for the applied dyes.

Efficient sintering of nanocrystalline titanium dioxide films for dye solar cells via raster scanning laser

NASA Astrophysics Data System (ADS)

Mincuzzi, Girolamo; Vesce, Luigi; Reale, Andrea; Di Carlo, Aldo; Brown, Thomas M.

2009-09-01

By identifying the right combination of laser parameters, in particular the integrated laser fluence Φ, we fabricated dye solar cells (DSCs) with UV laser-sintered TiO2 films exhibiting a power conversion efficiency η =5.2%, the highest reported for laser-sintered devices. η is dramatically affected by Φ and a clear trend is reported. Significantly, DSCs fabricated by raster scanning the laser beam to sinter the TiO2 films are made as efficient as those with oven-sintered ones. These results, confirmed on three batches of cells, demonstrate the remarkable potential (noncontact, local, low cost, rapid, selective, and scalable) of scanning laser processing applied to DSC technology.

Direct solar-pumped iodine laser amplifier

NASA Technical Reports Server (NTRS)

Han, K. S.

1986-01-01

During this period the parametric studies of the iodine laser oscillator pumped by a Vortek simulator were carried out before amplifier studies. The amplifier studies are postponed to the extended period after completing the parametric studies. In addition, the kinetic modeling of a solar-pumped iodine laser amplifier, and the experimental work for a solar pumped dye laser amplifier are in progress. This report contains three parts: (1) a 10 W CW iodine laser pumped by a Vortek solar simulator; (2) kinetic modeling to predict the time to lasing threshold, lasing time, and energy output of solar-pumped iodine laser; and (3) the study of the dye laser amplifier pumped by a Tamarack solar simulator.

High-repetition-rate, narrow-band dye lasers with water as a solvent for dyes

NASA Astrophysics Data System (ADS)

Ray, Alok K.; Sinha, Sucharita; Kundu, Soumitra; Kumar, Sasi; Nair, Sivagiriyal Karunakaran Sreenivasan; Pal, Tamal; Dasgupta, Kamalesh

2002-03-01

The performance of a copper vapor laser-pumped narrow-band dye laser in oscillator-amplifier configuration with water-based binary mixture solvents is described. Although oscillator efficiency in water-surfactant (sodium lauryl sulfate) solvent was comparable with that that employed pure ethanolic solvent, amplifier efficiency was found to be lower. Experiments that were carried out with vertically polarized pump beams and either horizontally or vertically polarized signal beams show that, in case of both the pump and signal having orthogonal polarization (horizontal) and same polarization (vertical), the extraction efficiency for both ethanolic and water-micelle media increased substantially from 15.7% to 18.5% and from 10% to 12.5%, respectively. However, the relative difference remained nearly the same, indicating that a slower orientational diffusion of excited dye molecules in a micellar medium is not responsible for a decrease in amplifier efficiency. Amplifier efficiency comparable with that containing ethanolic dye solutions could be obtained with a binary solvent that comprises a mixture of water and about 30% n-propanol. The performances of two efficient dyes, Rhodamine-6G and Kiton Red S, using water-based solvents were studied.

SERS activity of silver and gold nanostructured thin films deposited by pulsed laser ablation

NASA Astrophysics Data System (ADS)

Agarwal, N. R.; Tommasini, M.; Fazio, E.; Neri, F.; Ponterio, R. C.; Trusso, S.; Ossi, P. M.

2014-10-01

Nanostructured Au and Ag thin films were obtained by nanosecond pulsed laser ablation in presence of a controlled Ar atmosphere. Keeping constant other deposition parameters such as target-to-substrate distance, incidence angle, laser wavelength and laser fluence, the film morphology, revealed by SEM, ranges from isolated NPs to island structures and sensibly depends on gas pressure (10-100 Pa) and on the laser pulse number (500-3 × 10). The control of these two parameters allows tailoring the morphology and correspondingly the optical properties of the films. The position and width of the surface plasmon resonance peak, in fact, can be varied with continuity. The films showed remarkable surface-enhanced Raman activity (SERS) that depends on the adopted deposition conditions. Raman maps were acquired on micrometer-sized areas of both silver and gold substrates selected among those with the strongest SERS activity. Organic dyes of interest in cultural heritage studies (alizarin, purpurin) have been also considered for bench marking the substrates produced in this work. Also the ability to detect the presence of biomolecules was tested using lysozyme in a label free configuration.

Picosecond transient backward stimulated Raman scattering and pumping of femtosecond dye lasers

NASA Astrophysics Data System (ADS)

Arrivo, Steven M.; Spears, Kenneth G.; Sipior, Jeffrey

1995-02-01

We report studies of transient, backward stimulated, Raman scattering (TBSRS) in solvents with a 10 Hz, 27 ps, 532 nm pump laser. The TBSRS effect was used to create pulses at 545 nm and 630 nm with durations of 2-3 ps and 5-10 μJ of energy. The duration, energy and fluctuations of the Raman pulse were studied as a function of pump energy and focal parameters. A 5 μJ Raman pulse was amplified in either a Raman amplifier or two stage dye amplifier to 1 mJ levels. A 545 nm pulse of 3 ps duration was generated in CCl 4 and was then used to pump a short cavity dye laser (SCDL). The SCDL oscillator and a 5 stage dye amplifier provided a pulse of 700 fs and 400 μJ that was tunable near 590 nm.

DOE Office of Scientific and Technical Information (OSTI.GOV)

D'yachkov, Aleksei B; Labozin, Valerii P

The results of experiments on the 510 {yields} 578-nm conversion of high-power radiation from a copper vapour laser (CVL) in a dye cell are presented. The use of the efficient laser dye Pyrromethane 597 (PM-597) made it possible to convert the 120-W CVL radiation (72 W at 510 nm + 48 W at 578 nm) into 102-W radiation at 578 nm, which is equivalent to a conversion efficiency of 85%. Photostability of the dye in various solvents is studied. The photostability (more than 45 GJ mole{sup -1}) of PM-597 in n-heptane is found to be higher than that of Rhmore » 6G in ethanol. (control of laser radiation parameters)« less

Laser optogalvanic wavelength calibration with a commercial hollow cathode iron - neon discharge lamp

NASA Technical Reports Server (NTRS)

Zhu, Xinming; Nur, Abdullahi H.; Misra, Prabhakar

1994-01-01

351 optogalvanic transitions have been observed in the 337 - 598 nm wavelength region using an iron - neon hollow cathode discharge lamp and a pulsed tunable dye laser. 223 of these have been identified as transitions associated with neon energy levels. These optogalvanic transitions have allowed, in conjunction with interference fringes recorded concomitantly with an etalon, the calibration of the dye laser wavelength with 0.3/cm accuracy.

Analysis of violet-excited fluorochromes by flow cytometry using a violet laser diode.

PubMed

Telford, William G; Hawley, Teresa S; Hawley, Robert G

2003-07-01

Low power violet laser diodes (VLDs) have been evaluated as potential replacements for water-cooled argon-ion and krypton-ion ultraviolet and violet lasers for DNA content analysis using the Hoechst dyes and 4,6-diamidino-2-phenylindole (Shapiro HMN, Perlmutter NG: Cytometry 44:133-136, 2001). In this study, we used a VLD to excite a variety of violet-excited fluorescent molecules important in biomedical analysis, including the fluorochromes Cascade Blue and Pacific Blue, the expressible fluorescent protein cyan fluorescent protein (CFP), and the fluorogenic alkaline phosphatase (AP) substrate 2-(5'-chloro-2'-phosphoryloxyphenyl)-6-chloro-4-(3H)-quinazoline (ELF-97; for endogenous AP detection and cell surface labeling with AP-conjugated antibodies). Comparisons were made between VLD excitation and a krypton-ion laser emitting at 407 nm (both at higher power levels and with the beam attenuated at levels approximating the VLD) on the same FACSVantage SE stream-in-air flow cytometer. We evaluated a Power Technology 408-nm VLD (30 mW) equipped with circularization optics (18 mW maximum output, set to 15 mW) and a Coherent I-302C krypton-ion laser emitting at power levels ranging from 15 to 75 mW. Cascade Blue, Pacific Blue, and CFP showed comparable signal-to-noise ratios and levels of sensitivity with VLD excitation versus the krypton-ion laser at high and VLD-matched power outputs. Multicolor fluorescent protein analysis with 488-nm excitation of green fluorescent protein and DsRed and VLD excitation of CFP was therefore feasible and was demonstrated. Similar levels of excitation efficiency between krypton-ion and VLD sources also were observed for ELF-97 detection. These evaluations confirmed that VLDs may be cost- and maintenance-effective replacements for water-cooled gas lasers for applications requiring violet excitation in addition to DNA binding dyes. Published 2003 Wiley-Liss, Inc.

Ion yields in UV-MALDI mass spectrometry as a function of excitation laser wavelength and optical and physico-chemical properties of classical and halogen-substituted MALDI matrixes.

PubMed

Soltwisch, Jens; Jaskolla, Thorsten W; Hillenkamp, Franz; Karas, Michael; Dreisewerd, Klaus

2012-08-07

The laser wavelength constitutes a key parameter in ultraviolet-matrix-assisted laser desorption ionization-mass spectrometry (UV-MALDI-MS). Optimal analytical results are only achieved at laser wavelengths that correspond to a high optical absorption of the matrix. In the presented work, the wavelength dependence and the contribution of matrix proton affinity to the MALDI process were investigated. A tunable dye laser was used to examine the wavelength range between 280 and 355 nm. The peptide and matrix ion signals recorded as a function of these irradiation parameters are displayed in the form of heat maps, a data representation that furnishes multidimensional data interpretation. Matrixes with a range of proton affinities from 809 to 866 kJ/mol were investigated. Among those selected are the standard matrixes 2,5-dihydroxybenzoic acid (DHB) and α-cyano-4-hydroxycinnamic acid (HCCA) as well as five halogen-substituted cinnamic acid derivatives, including the recently introduced 4-chloro-α-cyanocinnamic acid (ClCCA) and α-cyano-2,4-difluorocinnamic acid (DiFCCA) matrixes. With the exception of DHB, the highest analyte ion signals were obtained toward the red side of the peak optical absorption in the solid state. A stronger decline of the molecular analyte ion signals generated from the matrixes was consistently observed at the low wavelength side of the peak absorption. This effect is mainly the result of increased fragmentation of both analyte and matrix ions. Optimal use of multiply halogenated matrixes requires adjustment of the excitation wavelength to values below that of the standard MALDI lasers emitting at 355 (Nd:YAG) or 337 nm (N(2) laser). The combined data provide new insights into the UV-MALDI desorption/ionization processes and indicate ways to improve the analytical sensitivity.

Comparison of in vivo and ex vivo laser scanning microscopy and multiphoton tomography application for human and porcine skin imaging

DOE Office of Scientific and Technical Information (OSTI.GOV)

Darvin, M E; Richter, H; Zhu, Y J

Two state-of-the-art microscopic optical methods, namely, confocal laser scanning microscopy in the fluorescence and reflectance regimes and multiphoton tomography in the autofluorescence and second harmonic generation regimes, are compared for porcine skin ex vivo and healthy human skin in vivo. All skin layers such as stratum corneum (SC), stratum spinosum (SS), stratum basale (SB), papillary dermis (PD) and reticular dermis (RD) as well as transition zones between these skin layers are measured noninvasively at a high resolution, using the above mentioned microscopic methods. In the case of confocal laser scanning microscopy (CLSM), measurements in the fluorescence regime were performed bymore » using a fluorescent dye whose topical application on the surface is well suited for the investigation of superficial SC and characterisation of the skin barrier function. For investigations of deeply located skin layers, such as SS, SB and PD, the fluorescent dye must be injected into the skin, which markedly limits fluorescence measurements using CLSM. In the case of reflection CLSM measurements, the obtained results can be compared to the results of multiphoton tomography (MPT) for all skin layers excluding RD. CLSM cannot distinguish between dermal collagen and elastin measuring their superposition in the RD. By using MPT, it is possible to analyse the collagen and elastin structures separately, which is important for the investigation of anti-aging processes. The resolution of MPT is superior to CLSM. The advantages and limitations of both methods are discussed and the differences and similarities between human and porcine skin are highlighted. (laser biophotonics)« less

[First clinical results using a pulsed dye laser Rhodamine 590 lithotripter in treatment of ureteral calculi].

PubMed

Jichlinski, P; Bonard, M; von Niederhäusern, W; Delacrétaz, G; Rink, K; Lambelet, P; Klohn, M; Bolle, J F; Graber, P

1991-09-01

A collaborative study about a pulsed dye laser Rhodamin 590 was undergone, 2 years ago, between the laser application center of EPFL and both urological departments of the university of Geneva and the university of Lausanne. First clinical results are presented. Ten patients have been treated for various ureteral stones, mainly calcium oxalate stones. Laser fragmentation was successful in seven cases. No serious complication was noted. Fragmentation efficiency seems better with a 320 microns fiber than with a 200 microns fiber.

Spectral evolution of distributed feedback laser of gold nanoparticles doped solid-state dye laser medium

NASA Astrophysics Data System (ADS)

An, N. T. M.; Lien, N. T. H.; Hoang, N. D.; Nghia, N. T.; Hoa, D. Q.

2017-10-01

Characteristics of suppressed relaxation oscillation of a distributed feedback dye laser (DFDL) based on the energy transfer process in a mixture of spherical gold nanoparticles-doped solid-state polymethylmetacrylate dissolved 4-(Dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran dye was theoretically and experimentally studied. Single pulse generation regime of the DFDL can be obtained with a suitable gold nanoparticle concentration and ratio of pump power over lasing threshold. Numerical analysis and experimental approach showed that in this regime, the first-pulse laser pulsewidth is rather unchanged while varying the gold nanoparticles concentration in the range of 2.0 × 109-2.0 × 1010 par cm-3. The enhancement of first pulse and the suppression of the secondary pulses by bi-direction energy transfer of spherical gold nanoparticles were experimentally observed.

A High Power, Frequency Tunable Colloidal Quantum Dot (CdSe/ZnS) Laser

PubMed Central

Prasad, Saradh; Saleh AlHesseny, Hanan; AlSalhi, Mohamad S.; Devaraj, Durairaj; Masilamai, Vadivel

2017-01-01

Tunable lasers are essential for medical, engineering and basic science research studies. Most conventional solid-state lasers are capable of producing a few million laser shots, but limited to specific wavelengths, which are bulky and very expensive. Dye lasers are continuously tunable, but exhibit very poor chemical stability. As new tunable, efficient lasers are always in demand, one such laser is designed with various sized CdSe/ZnS quantum dots. They were used as a colloid in tetrahydrofuran to produce a fluorescent broadband emission from 520 nm to 630 nm. The second (532 nm) and/or third harmonic (355 nm) of the Nd:YAG laser (10 ns, 10 Hz) were used together as the pump source. In this study, different sized quantum dots were independently optically pumped to produce amplified spontaneous emission (ASE) with 4 nm to 7 nm of full width at half-maximum (FWHM), when the pump power and focusing were carefully optimized. The beam was directional with a 7 mrad divergence. Subsequently, these quantum dots were combined together, and the solution was placed in a resonator cavity to obtain a laser with a spectral width of 1 nm and tunable from 510 to 630 nm, with a conversion efficiency of about 0.1%. PMID:28336863

A High Power, Frequency Tunable Colloidal Quantum Dot (CdSe/ZnS) Laser.

PubMed

Prasad, Saradh; AlHesseny, Hanan Saleh; AlSalhi, Mohamad S; Devaraj, Durairaj; Masilamai, Vadivel

2017-01-30

Tunable lasers are essential for medical, engineering and basic science research studies. Most conventional solid-state lasers are capable of producing a few million laser shots, but limited to specific wavelengths, which are bulky and very expensive. Dye lasers are continuously tunable, but exhibit very poor chemical stability. As new tunable, efficient lasers are always in demand, one such laser is designed with various sized CdSe/ZnS quantum dots. They were used as a colloid in tetrahydrofuran to produce a fluorescent broadband emission from 520 nm to 630 nm. The second (532 nm) and/or third harmonic (355 nm) of the Nd:YAG laser (10 ns, 10 Hz) were used together as the pump source. In this study, different sized quantum dots were independently optically pumped to produce amplified spontaneous emission (ASE) with 4 nm to 7 nm of full width at half-maximum (FWHM), when the pump power and focusing were carefully optimized. The beam was directional with a 7 mrad divergence. Subsequently, these quantum dots were combined together, and the solution was placed in a resonator cavity to obtain a laser with a spectral width of 1 nm and tunable from 510 to 630 nm, with a conversion efficiency of about 0.1%.

Optophysiological Approach to Resolve Neuronal Action Potentials with High Spatial and Temporal Resolution in Cultured Neurons

PubMed Central

Pagès, Stéphane; Côté, Daniel; De Koninck, Paul

2011-01-01

Cell to cell communication in the central nervous system is encoded into transient and local membrane potential changes (ΔVm). Deciphering the rules that govern synaptic transmission and plasticity entails to be able to perform Vm recordings throughout the entire neuronal arborization. Classical electrophysiology is, in most cases, not able to do so within small and fragile neuronal subcompartments. Thus, optical techniques based on the use of fluorescent voltage-sensitive dyes (VSDs) have been developed. However, reporting spontaneous or small ΔVm from neuronal ramifications has been challenging, in part due to the limited sensitivity and phototoxicity of VSD-based optical measurements. Here we demonstrate the use of water soluble VSD, ANNINE-6plus, with laser-scanning microscopy to optically record ΔVm in cultured neurons. We show that the sensitivity (>10% of fluorescence change for 100 mV depolarization) and time response (sub millisecond) of the dye allows the robust detection of action potentials (APs) even without averaging, allowing the measurement of spontaneous neuronal firing patterns. In addition, we show that back-propagating APs can be recorded, along distinct dendritic sites and within dendritic spines. Importantly, our approach does not induce any detectable phototoxic effect on cultured neurons. This optophysiological approach provides a simple, minimally invasive, and versatile optical method to measure electrical activity in cultured neurons with high temporal (ms) resolution and high spatial (μm) resolution. PMID:22016723

Novel receptor-targeted contrast agents for optical imaging of tumors

NASA Astrophysics Data System (ADS)

Becker, Andreas; Hessenius, Carsten; Bhargava, Sarah; Ebert, Bernd; Sukowski, Uwe; Rinneberg, Herbert H.; Wiedenmann, Bertram; Semmler, Wolfhard; Licha, Kai

2000-04-01

Many gastroenteropancreatic tumors express receptors for somatostatin (SST) and/or vasoactive intestinal peptide (VIP). These receptors can be used as molecular targets for the delivery of contrast agents for tumor diagnostics. We have synthesized conjugates consisting of a cyanine dye and an SST analogue or VIP for use as contrast agents in optical imaging. Receptor binding and internalization of these compounds were examined with optical methods in transfected RIN38 tumor cells expressing the SST2 receptor or a GFP- labeled VIP (VPAC1) receptor. Furthermore, biodistribution of the conjugates was examined by laser-induced fluorescence imaging in nude mice bearing SST2 or VPAC1 receptor- expressing tumors. After incubation of RIN38 SSTR2 cells in the presence of 100 nM indotricarbocyanine-SST analogue, cell-associated fluorescence increased, whereas no increase was observed when receptor-medicated endocytosis was inhibited. Indodicarbocyanine-VIP accumulated in RIN38 VPAC1 cells and co-localization with the GFP-labeled VPAC1 receptor was observed. After injection of indotricarbocyanine-SST analogue into tumor-bearing nude mice, SST2 receptor-positive tumors could be visualized for a time period from 10 min to at least 48 h. After application of indodicarbocyanine-VIP, a fluorescence signal in VIP1 receptor-expressing tumors was only detected during the first hour. We conclude that cyanine dye-labeled VIP and SST analogue are novel, targeted contrast agents for the optical imaging of tumors expressing the relevant receptor.

Multicolor fluorescence enhancement from a photonics crystal surface

NASA Astrophysics Data System (ADS)

Pokhriyal, A.; Lu, M.; Huang, C. S.; Schulz, S.; Cunningham, B. T.

2010-09-01

A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, fabricated uniformly from plastic materials over a ˜3×5 in.2 surface area by nanoreplica molding, utilizes two distinct resonant modes to enhance electric field stimulation of a dye excited by a λ =632.8 nm laser (cyanine-5) and a dye excited by a λ =532 nm laser (cyanine-3). Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle. Compared to detection of a dye-labeled protein on an ordinary glass surface, the photonic crystal surface exhibited a 32× increase in fluorescent signal intensity for cyanine-5 conjugated streptavidin labeling, while a 25× increase was obtained for cyanine-3 conjugated streptavidin labeling. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in the 532 nm<λ<633 nm range by selection of an appropriate incident angle. The device is designed for biological assays that utilize multiple fluorescent dyes within a single imaged area, such as gene expression microarrays.

Multicolor fluorescence enhancement from a photonics crystal surface

PubMed Central

Pokhriyal, A.; Lu, M.; Huang, C. S.; Schulz, S.; Cunningham, B. T.

2010-01-01

A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, fabricated uniformly from plastic materials over a ∼3×5 in.2 surface area by nanoreplica molding, utilizes two distinct resonant modes to enhance electric field stimulation of a dye excited by a λ=632.8 nm laser (cyanine-5) and a dye excited by a λ=532 nm laser (cyanine-3). Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle. Compared to detection of a dye-labeled protein on an ordinary glass surface, the photonic crystal surface exhibited a 32× increase in fluorescent signal intensity for cyanine-5 conjugated streptavidin labeling, while a 25× increase was obtained for cyanine-3 conjugated streptavidin labeling. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in the 532 nm<λ<633 nm range by selection of an appropriate incident angle. The device is designed for biological assays that utilize multiple fluorescent dyes within a single imaged area, such as gene expression microarrays. PMID:20957067

Delayed near-infrared analysis permits visualization of rodent retinal pigment epithelium layer in vivo

NASA Astrophysics Data System (ADS)

Pankova, Natalie; Zhao, Xu; Liang, Huiyuan; Baek, David Sung Hyeon; Wang, Hai; Boyd, Shelley

2014-07-01

Patches of atrophy of the retinal pigment epithelium (RPE) have not been described in rodent models of retinal degeneration, as they have the clinical setting using fundus autofluorescence. We hypothesize that prelabeling the RPE would increase contrast and allow for improved visualization of RPE loss in vivo. Here, we demonstrate a new technique termed "delayed near-infrared analysis (DNIRA)" that permits ready detection of rat RPE, using optical imaging in the near-infrared (IR) spectrum with aid of indocyanine green (ICG) dye. Using DNIRA, we demonstrate a fluorescent RPE signal that is detected using confocal scanning laser ophthalmoscopy up to 28 days following ICG injection. This signal is apparent only after ICG injection, is dose dependent, requires the presence of the ICG filters (795/810 nm excitation/emission), does not appear in the IR reflectance channel, and is eliminated in the presence of sodium iodate, a toxin that causes RPE loss. Rat RPE explants confirm internalization of ICG dye. Together with normal retinal electrophysiology, these findings demonstrate that DNIRA is a new and safe noninvasive optical imaging technique for in vivo visualization of the RPE in models of retinal disease.

Detection of volatile organic compounds through a sensing film of TiO II doped with organic dyes deposited on an optical fiber

NASA Astrophysics Data System (ADS)

Muñoz A., S.; Ramos M., J.; Martínez H., C.; Castillo M., J.; Beltrán P., G.; Palomino M., R.

2007-03-01

The necessity of detection and recognition of different types of gases, such as volatile organic compounds, which are frequently found in food and beverage industries among others, requires the development of different types of sensors. In this work, an application of optical fiber for the detection of volatile organic compounds, particularly ethanol is presented. The sensor was constructed removing a portion of the cladding and depositing instead a sensing titanium dioxide (TiO II) film doped with an organic dye (rhodamine 6G) by the sol-gel method. The sensor response was measured in a Teflon chamber where the sample to be measured was injected. A He-Ne laser beam was coupled to the fiber and the variation in the output power was measured which indicates the gas presence. The difference between the output power with and without gas gives a measure of the concentration that exists in the chamber. The experimental results showed that for an ethanol concentration range from 0 to 10500 ppm, the response of the sensor was approximately linear with a correlation coefficient of 0.9924.

Multi-modal in vivo imaging of brain blood oxygenation, blood flow and neural calcium dynamics during acute seizures

NASA Astrophysics Data System (ADS)

Ringuette, Dene; Jeffrey, Melanie A.; Carlen, Peter L.; Levi, Ofer

2016-03-01

Dysfunction of the vascular endothelium has been implicated in the development of epilepsy. To better understand the relation between vascular function and seizure and provide a foundation for interpreting results from functional imaging in chronic disease models, we investigate the relationship between intracellular calcium dynamics and local cerebral blood flow and blood oxygen saturation during acute seizure-like events and pharmacological seizure rescue. To probe the relation between the aforementioned physiological markers in an acute model of epilepsy in rats, we integrated three different optical modalities together with electrophysiological recordings: Laser speckle contrast imaging (LSCI) was used to study changes in flow speeds, Intrinsic optical signal imaging (IOSI) was used to monitor changes in oxygenated, de-oxygenated, and total hemoglobin concentration, and Calcium-sensitive dye imaging was used to monitor intracellular calcium dynamics. We designed a dedicated cortical flow chamber to remove superficial blood and dye resulting from the injection procedure, which reduced spurious artifacts. The near infrared light used for IOSI and LSCI was delivered via a light pipe integrated with the flow chamber to minimize the effect of fluid surface movement on illumination stability. Calcium-sensitive dye was injected via a glass electrode used for recording the local field potential. Our system allowed us to observe and correlate increases in intracellular calcium, blood flow and blood volume during seizure-like events and provide a quantitative analysis of neurovascular coupling changes associated with seizure rescue via injection of an anti-convulsive agent.

Pulsed laser-induced damage of metals at 492 nm.

PubMed

Marrs, C D; Faith, W N; Dancy, J H; Porteus, J O

1982-11-15

A triaxial flashlamp-pumped dye laser has been used to perform laser damage testing of metal surfaces in the blue-green spectral region. Using LD490 laser dye, the laser produces 0.18-J, 0.5-microsec pulses at 492 nm. The spatial profile of the focused beam is measured in orthogonal directions in the plane of the sample surface. The orthogonal profiles are flat-topped Gaussians with 1/e(2) widths of 270 microm. Multithreshold laser damage test results are presented for polished Mo, diamond-turned high-purity Al alloy, diamond-turned bulk Cu, and diamond-turned electrodeposits of Ag and Au on Cu. Comparisons are made between calculated and experimentally measured slip and melt thresholds.

A Plasma Ultraviolet Source for Short Wavelength Lasers.

DTIC Science & Technology

1988-04-15

plasma focus (DPF) device was evaluated for the feasibility of blue-green and near ultraviolet laser pumping. As the result of optimizing the operating conditions of DPF and laser system, the maximum untuned laser output exceeded 4.0mJ corresponding to the energy density 8.3J/liter which is much higher than the typical flashlamp dye laser. The spectral irradiance of DPF at the absorption bands for LD390 and LD490 were 5.5W/sq cm-nm, 0.3W.sq cm-nm, respectively. Due to the lower pump power of DPF at 355nm than the threshold of LD390, the laser pumping of LD390 dye was not

High-speed off-axis holographic cinematography with a copper-vapor-pumped dye laser.

PubMed

Lauterborn, W; Judt, A; Schmitz, E

1993-01-01

A series of coherent light pulses is generated by pumping a dye laser with the pulsed output of a copper-vapor laser at rates of as much as 20 kHz. Holograms are recorded at this pulse rate on a rotating holographic plate. This technique of high-speed holographic cinematography is demonstrated by viewing the bubble filaments that appear in water under the action of a sound field of high intensity.

DNA-based dye lasers: progress in this half a decade

NASA Astrophysics Data System (ADS)

Kawabe, Yutaka

2016-09-01

After the invention of DNA-surfactant films and the proposal of dye doping into them by Ogata, many applications were demonstrated. Among them tunable thin film laser is one of the most attractive functional devices. Development and progress in DNA based lasers after the first observation of amplified spontaneous emission (ASE) by us has been reviewed in a former paper published in 2011.1 In this proceeding, progresses in the subsequent half a decade are described.

Evaluation of OH laser-induced fluorescence techniques for supersonic combustion diagnostics

NASA Technical Reports Server (NTRS)

Quagliaroli, T. M.; Laufer, G.; Krauss, R. H.; Mcdaniel, J. C., Jr.

1992-01-01

The limitations on application of dye laser and narrowband tunable KrF excimer laser systems to planar OH fluorescence measurements in supersonic combustion test facilities are examined. Included in the analysis are effects of collisional quenching, beam absorption, fluorescence trapping, and signal strengths on achievable measurement accuracy using several excitation and detection options for either of the two laser systems. Dye-based laser systems are found to be the method of choice for imaging OH concentrations less than 10 exp 15 per cu cm, while the KrF based systems provide significant reduction in measurement ambiguity for concentrations in excess of 10 exp 15 per cu cm.

Near-infrared voltage-sensitive fluorescent dyes optimized for optical mapping in blood-perfused myocardium.

PubMed

Matiukas, Arvydas; Mitrea, Bogdan G; Qin, Maochun; Pertsov, Arkady M; Shvedko, Alexander G; Warren, Mark D; Zaitsev, Alexey V; Wuskell, Joseph P; Wei, Mei-de; Watras, James; Loew, Leslie M

2007-11-01

Styryl voltage-sensitive dyes (e.g., di-4-ANEPPS) have been used successfully for optical mapping in cardiac cells and tissues. However, their utility for probing electrical activity deep inside the myocardial wall and in blood-perfused myocardium has been limited because of light scattering and high absorption by endogenous chromophores and hemoglobin at blue-green excitation wavelengths. The purpose of this study was to characterize two new styryl dyes--di-4-ANBDQPQ (JPW-6003) and di-4-ANBDQBS (JPW-6033)--optimized for blood-perfused tissue and intramural optical mapping. Voltage-dependent spectra were recorded in a model lipid bilayer. Optical mapping experiments were conducted in four species (mouse, rat, guinea pig, and pig). Hearts were Langendorff perfused using Tyrode's solution and blood (pig). Dyes were loaded via bolus injection into perfusate. Transillumination experiments were conducted in isolated coronary-perfused pig right ventricular wall preparations. The optimal excitation wavelength in cardiac tissues (650 nm) was >70 nm beyond the absorption maximum of hemoglobin. Voltage sensitivity of both dyes was approximately 10% to 20%. Signal decay half-life due to dye internalization was 80 to 210 minutes, which is 5 to 7 times slower than for di-4-ANEPPS. In transillumination mode, DeltaF/F was as high as 20%. In blood-perfused tissues, DeltaF/F reached 5.5% (1.8 times higher than for di-4-ANEPPS). We have synthesized and characterized two new near-infrared dyes with excitation/emission wavelengths shifted >100 nm to the red. They provide both high voltage sensitivity and 5 to 7 times slower internalization rate compared to conventional dyes. The dyes are optimized for deeper tissue probing and optical mapping of blood-perfused tissue, but they also can be used for conventional applications.

Self-healing of optical functions by molecular metabolism in a swollen elastomer

NASA Astrophysics Data System (ADS)

Saito, Mitsunori; Nishimura, Tatsuya; Sakiyama, Kohei; Inagaki, Sota

2012-12-01

Optical functions of organic dyes, e.g., fluorescence or photochromism, tend to degrade by light irradiation, which causes a short lifetime of photonic devices. Self-healing of optical functions is attainable by metabolizing bleached molecules with nonirradiated ones. A polydimethylsiloxane elastomer provides a useful matrix for dye molecules, since its flexible structure with nano-sized intermolecular spaces allows dye diffusion from a reservoir to an operation region. Swelling the elastomer with a suitable solvent promotes both dissolution and diffusion of dye molecules. This self-healing function was demonstrated by an experiment in which a photochromic elastomer exhibited improved durability against a repeated coloring-decoloring process.

Electrically controllable liquid crystal random lasers below the Fréedericksz transition threshold.

PubMed

Lee, Chia-Rong; Lin, Jia-De; Huang, Bo-Yuang; Lin, Shih-Hung; Mo, Ting-Shan; Huang, Shuan-Yu; Kuo, Chie-Tong; Yeh, Hui-Chen

2011-01-31

This investigation elucidates for the first time electrically controllable random lasers below the threshold voltage in dye-doped liquid crystal (DDLC) cells with and without adding an azo-dye. Experimental results show that the lasing intensities and the energy thresholds of the random lasers can be decreased and increased, respectively, by increasing the applied voltage below the Fréedericksz transition threshold. The below-threshold-electric-controllability of the random lasers is attributable to the effective decrease of the spatial fluctuation of the orientational order and thus of the dielectric tensor of LCs by increasing the electric-field-aligned order of LCs below the threshold, thereby increasing the diffusion constant and decreasing the scattering strength of the fluorescence photons in their recurrent multiple scattering. This can result in the decrease in the lasing intensity of the random lasers and the increase in their energy thresholds. Furthermore, the addition of an azo-dye in DDLC cell can induce the range of the working voltage below the threshold for the control of the random laser to reduce.

Techniques for reducing and/or eliminating secondary modes in a dye laser oscillator

DOEpatents

Hackel, Richard P.

1988-01-01

A dye laser master oscillator is disclosed herein. This oscillator is intended to provide a single mode output, that is, a primary beam of light of a specific wavelength, but also has the tendency to provide secondary modes, that is, secondary beams of light at different wavelengths and slightly off-axis with respect to the primary beam as a result of grazing incident reflections within the dye cell forming part of the master oscillator. Also disclosed herein are a number of different techniques for reducing or eliminating these secondary modes.

Efficient 7-J flashlamp-pumped dye laser at 500-nm wavelength

DOE Office of Scientific and Technical Information (OSTI.GOV)

Everett, P.N.; Aldag, H.R.; Ehrlich, J.J.

1986-07-01

An existing transverse flow flashlamp-pumped dye laser capable of operation at 500 pps for extended periods of time has been modified and optimized for operation at 502 nm using coumarin 504. Energies of over 7 J/ pulse and efficiencies of over 1% have been demonstrated in single-shot operation. This has been achieved by using a spectral transfer dye in the flashlamp coolant to increase the useful output of the flashlamps. Flashlamps were tested at up to 400-J input per lamp for extended periods to develop lamp life data.

On the possibility of measuring atmospheric OH using intracavity laser spectroscopy

NASA Technical Reports Server (NTRS)

Mcmanus, J. Barry; Kolb, C. E.

1994-01-01

Intracavity laser spectroscopy (ILS) has been demonstrated to be useful for measuring extremely weak absorption produced by gases in air. ILS is based on the observation that when there are spectrally narrow losses within the cavity of a broadband laser, the laser output has corresponding spectral holes where the laser oscillation is partially quenched. The depth of the laser output dips can be enhanced by a factor of 10(exp 5) over the depth of the initial cavity loss, and absorptivities of 10(exp -8) cm(exp -1) have been measured in lasers only one meter long. With ILS, one can achieve in a compact space a spectral contrast that would otherwise require kilometers of pathlength. ILS systems typically use quasi-continuous wave dye lasers operating close to threshold. The pump laser is modulated from just below to just above the threshold level for the dye laser, and the dye laser output is spectroscopically observed during a well defined time interval after the onset of lasing (the generation time). The spectral contrast of an intracavity absorber is equivalent to that produced by absorption through a path length equal to the generation time multiplied by the speed of light (assuming the cavity is completely filed with the absorber) up to some limiting time. Thus, if one measures the spectrum after 33 microseconds, the effective path length is 10,000 meters.

Application of Raman spectroscopy and surface-enhanced Raman scattering to the analysis of synthetic dyes found in ballpoint pen inks.

PubMed

Geiman, Irina; Leona, Marco; Lombardi, John R

2009-07-01

The applicability of Raman spectroscopy and surface-enhanced Raman scattering (SERS) to the analysis of synthetic dyes commonly found in ballpoint inks was investigated in a comparative study. Spectra of 10 dyes were obtained using a dispersive system (633 nm, 785 nm lasers) and a Fourier transform system (1064 nm laser) under different analytical conditions (e.g., powdered pigments, solutions, thin layer chromatography [TLC] spots). While high fluorescence background and poor spectral quality often characterized the normal Raman spectra of the dyes studied, SERS was found to be generally helpful. Additionally, dye standards and a single ballpoint ink were developed on a TLC plate following a typical ink analysis procedure. SERS spectra were successfully collected directly from the TLC plate, thus demonstrating a possible forensic application for the technique.

Real-time association rate constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes

NASA Astrophysics Data System (ADS)

Simmonds, Boris; Wang, Chun-Wei; Kapoor, Rakesh

2010-02-01

This document reports a novel method of measuring association rate constant (ka) for antibody-antigen interaction using evanescent wave-based combination tapered fiber-optic biosensor (CTFOB) dip-probes. The method was demonstrated by measuring association rate constant for bovine serum albumin (BSA) and anti-BSA antibody interaction. "Direct method" was used for detection; goat anti-BSA "capture" antibodies were immobilized on the probe surfaces while the antigen (BSA) was directly labeled with Alexa 488 dye. The probes were subsequently submerged in 3nM Labeled BSA in egg albumin (1 mg/ml). The fluorescence signal recorded was proportional to BSA anti-BSA conjugates and continuous signal was acquired suing a fiber optic spectrometer (Ocean Optics, Inc.). A 476 nm diode laser was use as an excitation source. Association constant was estimated from a plot of signal as a function of time. Measured association rate constant ka for the binding of BSA with anti-BSA at room temperature is (8.33 +/- 0.01) x 104 M-1s-1.

Design considerations for multi component molecular-polymeric nonlinear optical materials

NASA Astrophysics Data System (ADS)

Singer, K. D.; Kuzyk, M. G.; Fang, T.; Holland, W. R.; Cahill, P. A.

1990-08-01

We review our work on multi component polymeric nonlinear optical materials. These materials consist of nonlinear optical molecules incorporated in a polymeric host. A cross-linked triazine polymer incorporating a dicyanovinyl terminated azo dye was found to be relatively stable at 85 deg and possess an electro-optic coefficient of 11pm/V. We have also observed the zero dispersion condition in a new anomalous dispersion dye for phase matched second harmonic generation, and expect efficient conversion to the blue. A squarylium dye, ISQ, has been found to possess a large third order nonlinearity, and may display two-level behavior.

Guest-host polymer fibers for nonlinear optics

NASA Astrophysics Data System (ADS)

Kuzyk, M. G.; Paek, U. C.; Dirk, C. W.

1991-08-01

We report on the fabrication of poly(methyl methacrylate) (PMMA) nonlinear optical fibers with dye-doped cores. The dye-doped cores have an elevated refractive index that defines a waveguiding region with a large third-order susceptibility and with single-mode dimensions. The measured third-order susceptibility of a squarylium-doped PMMA film material and the measured optical loss of the dye-doped fiber core results in a figure of merit that is suitable for all-optical device applications at λ=1.3 μm. The impact of further improvements in PMMA loss and chromophore nonlinearity are also discussed.

1983 Annual Report on Laser Dyes.

DTIC Science & Technology

1984-04-01

Dye II). lThe laser portitm f 1327 11982) 311 the work was sLpp orted b , the National Aerollautics 10 (; II. \\s , .K. Jack-1 amnd AMI . ialpern. (hem...to be the longest-lived. ni)lciatc iu,pui , laser dive solution ol ami that hawe been reported. 1. Introduction We itade a tnore lhiirough evaluation...J. E. Colbert 01) E. White (1) I Riverside Research Institute. Arlington, VA (Suite 714. R. Pamst) 2 Riverside Research Institute. New York, NY Dr. M

Thermal damage control of dye-assisted laser tissue welding: effect of dye concentration

NASA Astrophysics Data System (ADS)

Xie, Hua; Buckley, Lisa A.; Prahl, Scott A.; Shaffer, Brian S.; Gregory, Kenton W.

2001-05-01

Successful laser-assisted tissue welding was implemented to provide proper weld strength with minimized tissue thermal injury. We investigated and compared the weld strengths and morphologic changes in porcine small intestinal submucose (SIS) and porcine ureteral tissues with various concentration of indocyanine green (ICG) and with a solid albumin sheet. The study showed that the tissues were welded at lower ICG concentration (0.05 mM) with minimized tissue thermal damage using an 800-nm wavelength diode laser.

Method and apparatus for passive optical dosimeter comprising caged dye molecules

DOEpatents

Sandison, David R.

2001-07-03

A new class of ultraviolet dosimeters is made possible by exposing caged dye molecules, which generate a dye molecule on exposure to ultraviolet radiation, to an exterior environment. Applications include sunburn monitors, characterizing the UV exposure history of UV-sensitive materials, especially including structural plastics, and use in disposable `one-use` optical equipment, especially medical devices.

High-resolution imaging using endoscopic holography

NASA Astrophysics Data System (ADS)

Bjelkhagen, Hans I.

1990-08-01

Endoscopic holography or endoholography combines the features of endoscopy and holography. The purpose of endoholographic imaging is to provide the physician with a unique means of extending diagnosis by providing a life-like record of tissue. Endoholographic recording will provide means for microscopic examination of tissue and in some cases may obviate the need to excise specimens for biopsy. In this method holograms which have the unique properties of three-dimensionality large focal depth and high resolution are made with a newly designed endoscope. The endoscope uses a single-mode optical fiber for illumination and single-beam reflection holograms are recorded in close contact with the tissue at the distal end of the endoscope. The holograms are viewed under a microscope. By using the proper combinations of dyes for staining specific tissue types with various wavelengths of laser illumination increased contrast on the cellular level can be obtained. Using dyes such as rose bengal in combination with the 514. 5 nm line of an argon ion laser and trypan blue or methylene blue with the 647. 1 nm line of a krypton ion laser holograms of the stained colon of a dog showed the architecture of the colon''s columnar epithelial cells. It is hoped through chronological study using this method in-vivo an increased understanding of the etiology and pathology of diseases such as Crohn''s diseases colitis proctitis and several different forms of cancer will help to their control. 1.

Third-Order Optical Nonlinearities of Squarylium Dyes with Benzothiazole Donor Groups Measured Using the Picosecond Z-Scan Technique

NASA Astrophysics Data System (ADS)

Li, Zhong-Yu; Xu, Song; Chen, Zi-Hui; Zhang, Fu-Shi; Kasatani, Kazuo

2011-08-01

Third-order optical nonlinearities of two squarylium dyes with benzothiazole donor groups (BSQ1 and BSQ2) in chloroform solution are measured by a picosecond Z-scan technique at 532 nm. It is found that the two compounds show the saturation absorption and nonlinear self-focus refraction effect. The molecular second hyperpolarizabilities are calculated to be 7.46 × 10-31 esu and 5.01 × 10-30 esu for BSQ1 and BSQ2, respectively. The large optical nonlinearities of squarylium dyes can be attributed to their rigid and intramolecular charge transfer structure. The difference in γ values is attributed to the chloro group of benzene rings of BSQ2 and the one-photon resonance effect. It is found that the third-order nonlinear susceptibilities of two squarylium dyes are mainly determined by the real parts of χ(3), and the large optical nonlinearities of studied squarylium dyes can be attributed to the nonlinear refraction.

Continuously tunable microdroplet-laser in a microfluidic channel.

PubMed

Tang, Sindy K Y; Derda, Ratmir; Quan, Qimin; Lončar, Marko; Whitesides, George M

2011-01-31

This paper describes the generation and optical characterization of a series of dye-doped droplet-based optical microcavities with continuously decreasing radius in a microfluidic channel. A flow-focusing nozzle generated the droplets (~21 μm in radius) using benzyl alcohol as the disperse phase and water as the continuous phase. As these drops moved down the channel, they dissolved, and their size decreased. The emission characteristics from the drops could be matched to the whispering gallery modes from spherical micro-cavities. The wavelength of emission from the drops changed from 700 to 620 nm as the radius of the drops decreased from 21 μm to 7 μm. This range of tunability in wavelengths was larger than that reported in previous work on droplet-based cavities.

Advances in solid state laser technology for space and medical applications

NASA Technical Reports Server (NTRS)

Byvik, C. E.; Buoncristiani, A. M.

1988-01-01

Recent developments in laser technology and their potential for medical applications are discussed. Gas discharge lasers, dye lasers, excimer lasers, Nd:YAG lasers, HF and DF lasers, and other commonly used lasers are briefly addressed. Emerging laser technology is examined, including diode-pumped lasers and other solid state lasers.

Using the ultra-long pulse width pulsed dye laser and elliptical spot to treat resistant nasal telangiectasia.

PubMed

Madan, Vishal; Ferguson, Janice

2010-01-01

Thick linear telangiectasia on the ala nasi and nasolabial crease can be resistant to treatment with the potassium-titanyl-phosphate (KTP) laser and the traditional round spot on a pulsed dye laser (PDL). We evaluated the efficacy of a 3 mm x 10 mm elliptical spot using the ultra-long pulse width on a Candela Vbeam(R) PDL for treatment of PDL- and KTP laser-resistant nasal telangiectasia. Nasal telangiectasia resistant to PDL (12 patients) and KTP laser (12 patients) in 18 patients were treated with a 3 mm x 10 mm elliptical spot on the ultra-long pulse pulsed dye laser (ULPDL) utilising long pulse width [595 nm, 40 ms, double pulse, 30:20 dynamic cooling device (DCD)]. Six patients had previously received treatment with both PDL and KTP laser prior to ULPDL (40 treatments, range1-4, mean 2.2). Complete clearance was seen in ten patients, and eight patients displayed more than 80% improvement after ULPDL treatment. Self-limiting purpura occurred with round spot PDL and erythema with KTP laser and ULPDL. Subtle linear furrows along the treatment sites were seen in three patients treated with the KTP laser. ULPDL treatment delivered using a 3 mm x 10 mm elliptical spot was non-purpuric and highly effective in the treatment of nasal telangiectasia resistant to KTP laser and PDL.

Micromanipulation by laser microbeam and optical tweezers: from plant cells to single molecules.

PubMed

Greulich, K O; Pilarczyk, G; Hoffmann, A; Meyer Zu Hörste, G; Schäfer, B; Uhl, V; Monajembashi, S

2000-06-01

Complete manipulation by laser light allows precise and gentle treatment of plant cells, subcellular structures, and even individual DNA molecules. Recently, affordable lasers have become available for the construction of microbeams as well as for optical tweezers. This may generate new interest in these tools for plant biologists. Early experiments, reviewed in this journal, showed that laser supported microinjection of material into plant cells or tissues circumvents mechanical problems encountered in microinjection by fragile glass capillaries. Plant protoplasts could be fused with each other when under microscopical observation, and it was no major problem to generate a triple or quadruple fusion product. In the present paper we review experiments where membrane material was prepared from root hair tips and microgravity was simulated in algae. As many plant cells are transparent, it is possible to work inside living, intact cells. New experiments show that it is possible to release by optical micromanipulation, with high spatial resolutions, intracellular calcium from caged compounds and to study calcium oscillations. An example for avian cardiac tissue is given, but the technique is also suitable for plant cell research. As a more technical tool, optical tweezers can be used to spatially fix subcellular structures otherwise moving inside a cell and thus make them available for investigation with a confocal microscope even when the time for image formation is extended (for example at low fluorescence emission). A molecular biological example is the handling of chromosomes and isolated individual DNA molecules by laser microtools. For example, chromosomes can be cut along complex trajectories, not only perpendicular to their long axis. Single DNA molecules are cut by the laser microbeam and, after coupling such a molecule to a polystrene microbead, are handled in complex geometries. Here, the individual DNA molecules are made visible with a conventional fluorescence microscope by fluorescent dyes such as SYBRGreen. The cutting of a single DNA molecule by molecules of the restriction endonuclease EcoRI can be observed directly, i.e. a type of single molecule restriction analysis is possible. Finally, mechanical properties of individual DNA molecules can be observed directly.

Tuning the photonic band gap in cholesteric liquid crystals by temperature-dependent dopant solubility.

PubMed

Huang, Yuhua; Zhou, Ying; Doyle, Charlie; Wu, Shin-Tson

2006-02-06

We have investigated the physical and optical properties of the left-handed chiral dopant ZLI-811 mixed in a nematic liquid crystal (LC) host BL006. The solubility of ZLI-811 in BL006 at room temperature is ~24 wt%, but can be enhanced by increasing the temperature. Consequently, the photonic band gap of the cholesteric liquid crystal (CLC) mixed with more than 24 wt% chiral dopant ZLI-811 is blue shifted as the temperature increases. Based on this property, we demonstrate two applications in thermally tunable band-pass filters and dye-doped CLC lasers.

Inverse relaxation effect of azo-dye molecules: The role of the film anisotropy

NASA Astrophysics Data System (ADS)

Sehnem, A. L.; Faita, F. L.; Cabrera, F. C.; Job, A. E.; Bechtold, I. H.

2013-11-01

We investigated the effect generally treated in the literature as inverse relaxation, which is related to an increase in the birefringence of azopolymer films after the inscription laser is turned off. The results demonstrate that films prepared with the casting method on anisotropic substrates induce a preferential organization of the polymeric chains. Inverse relaxation is evidenced only when the photo-alignment of the azo groups is induced parallel to the orientation of the polymeric chains. Thus, it is possible to enhance the optical storage in these systems with appropriate alignment methods.

Direct solar-pumped iodine laser amplifier

NASA Technical Reports Server (NTRS)

Han, K. S.

1985-01-01

This semiannual progress report covers the period from April 1, 1985 to Sept. 30, 1985 under NASA grant NAS1-441 entitled direct solar pumped iodine laser amplifier. During this period the parametric studies of the iodine laser oscillator pumped by a Vortek simulator was carried out before the amplifier studies. The amplifier studies are postponed to the extended period following completion of the parametric studies. In addition, the kinetic modeling of a solar pumped iodine laser amplifier, and the experimental work for a solar pumped dye laser amplifier are in progress. This report contains three parts: (1) the radiation characteristics of solar simulator and the parametric characteristics of photodissociation iodine laser continuously pumped by a Vortek solar simulator; (2) kinetic modeling of a solar pumped iodine laser amplifier; and (3) the study of the dye laser amplifier pumped by a Tamarack solar simulator.

Photoluminescence analysis of self induced planer alignment in azo dye dispersed nematic liquid crystal complex

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kumar, Rishi, E-mail: kkraina@gmail.com; Sood, Srishti, E-mail: kkraina@gmail.com; Raina, K. K., E-mail: kkraina@gmail.com

2014-04-24

We have developed azo dye doped nematic liquid crystal complex for advanced photonic liquid crystal display technology aspects. Disperse orange azo dye self introduced planer alignment in the nematic liquid crystal without any surface anchoring treatment. Planer alignment was characterized by optical polarizing microscopy. The electro-optical switching response of dye disperse planer aligned nematic cell was investigated as a function of applied voltage with the help of photoluminescence spectrophotometer for the tuning of photoluminescence contrast.

Reconfigurable Solid-state Dye-doped Polymer Ring Resonator Lasers

NASA Astrophysics Data System (ADS)

Chandrahalim, Hengky; Fan, Xudong

2015-12-01

This paper presents wavelength configurable on-chip solid-state ring lasers fabricated by a single-mask standard lithography. The single- and coupled-ring resonator hosts were fabricated on a fused-silica wafer and filled with 3,3‧-Diethyloxacarbocyanine iodide (CY3), Rhodamine 6G (R6G), and 3,3‧-Diethylthiadicarbocyanine iodide (CY5)-doped polymer as the reconfigurable gain media. The recorded lasing threshold was ~220 nJ/mm2 per pulse for the single-ring resonator laser with R6G, marking the lowest threshold shown by solid-state dye-doped polymer lasers fabricated with a standard lithography process on a chip. A single-mode lasing from a coupled-ring resonator system with the lasing threshold of ~360 nJ/mm2 per pulse was also demonstrated through the Vernier effect. The renewability of the dye-doped polymer was examined by removing and redepositing the dye-doped polymer on the same resonator hosts for multiple cycles. We recorded consistent emissions from the devices for all trials, suggesting the feasibility of employing this technology for numerous photonic and biochemical sensing applications that entail for sustainable, reconfigurable, and low lasing threshold coherent light sources on a chip.

Reconfigurable Solid-state Dye-doped Polymer Ring Resonator Lasers

PubMed Central

Chandrahalim, Hengky; Fan, Xudong

2015-01-01

This paper presents wavelength configurable on-chip solid-state ring lasers fabricated by a single-mask standard lithography. The single- and coupled-ring resonator hosts were fabricated on a fused-silica wafer and filled with 3,3′-Diethyloxacarbocyanine iodide (CY3), Rhodamine 6G (R6G), and 3,3′-Diethylthiadicarbocyanine iodide (CY5)-doped polymer as the reconfigurable gain media. The recorded lasing threshold was ~220 nJ/mm2 per pulse for the single-ring resonator laser with R6G, marking the lowest threshold shown by solid-state dye-doped polymer lasers fabricated with a standard lithography process on a chip. A single-mode lasing from a coupled-ring resonator system with the lasing threshold of ~360 nJ/mm2 per pulse was also demonstrated through the Vernier effect. The renewability of the dye-doped polymer was examined by removing and redepositing the dye-doped polymer on the same resonator hosts for multiple cycles. We recorded consistent emissions from the devices for all trials, suggesting the feasibility of employing this technology for numerous photonic and biochemical sensing applications that entail for sustainable, reconfigurable, and low lasing threshold coherent light sources on a chip. PMID:26674508

Reconfigurable Solid-state Dye-doped Polymer Ring Resonator Lasers.

PubMed

Chandrahalim, Hengky; Fan, Xudong

2015-12-17

This paper presents wavelength configurable on-chip solid-state ring lasers fabricated by a single-mask standard lithography. The single- and coupled-ring resonator hosts were fabricated on a fused-silica wafer and filled with 3,3'-Diethyloxacarbocyanine iodide (CY3), Rhodamine 6G (R6G), and 3,3'-Diethylthiadicarbocyanine iodide (CY5)-doped polymer as the reconfigurable gain media. The recorded lasing threshold was ~220 nJ/mm(2) per pulse for the single-ring resonator laser with R6G, marking the lowest threshold shown by solid-state dye-doped polymer lasers fabricated with a standard lithography process on a chip. A single-mode lasing from a coupled-ring resonator system with the lasing threshold of ~360 nJ/mm(2) per pulse was also demonstrated through the Vernier effect. The renewability of the dye-doped polymer was examined by removing and redepositing the dye-doped polymer on the same resonator hosts for multiple cycles. We recorded consistent emissions from the devices for all trials, suggesting the feasibility of employing this technology for numerous photonic and biochemical sensing applications that entail for sustainable, reconfigurable, and low lasing threshold coherent light sources on a chip.

Analysis of in vivo penetration of textile dyes causing allergic reactions

NASA Astrophysics Data System (ADS)

Lademann, J.; Patzelt, A.; Worm, M.; Richter, H.; Sterry, W.; Meinke, M.

2009-10-01

Contact allergies to textile dyes are common and can cause severe eczema. In the present study, we investigated the penetration of a fluorescent textile dye, dissolved from a black pullover, into the skin of one volunteer during perspiration and nonperspiration. Previously, wearing this pullover had induced a severe contact dermatitis in an 82-year old woman, who was not aware of her sensitization to textile dyes. The investigations were carried out by in vivo laser scanning microscopy. It could be demonstrated that the dye was eluted from the textile material by sweat. Afterwards, the dye penetrated into the stratum corneum and into the hair follicles. Inside the hair follicles, the fluorescent signal was still detectable after 24 h, whereas it was not verifiable anymore in the stratum corneum, Laser scanning microscopy represents an efficient tool for in vivo investigation of the penetration and storage of topically applied substances and allergens into the human skin and reveals useful hints for the development and optimization of protection strategies.

Solar generation and storage of O2 (a 1 delta g)

NASA Technical Reports Server (NTRS)

Twarowski, Allen J.; Dao, Phan; Good, Lisa A.

1988-01-01

An investigation was performed of the technical steps required to design a solar powered oxygen-iodine laser. Singlet delta oxygen is formed upon transfer of energy from selected photoexcited dye molecules to ground state molecular oxygen and then is concentrated and stored as an endoperoxide by reaction with an aromatic hydrocarbon. The endoperoxide, when heated, releases singlet oxygen in high yield thus providing a regenerable source of laser fuel. Energy transfer from dye molecules to molecular oxygen was investigated. When dye molecules were adsorbed to polymer substrates it was observed that the dye became embedded in the polymer matrix. Porphin dyes were incorporated into films of 1,4-dimethyl-2-poly(vinylnaphthalene), 2PVN. An endoperoxide was formed when porphin-doped 2PVN was exposed to visible radiation. This demonstrates the possibility of generating singlet oxygen using solar energy and concentrating and storing it in one simple step. Transport of energy by exciton migration in polycrystalline dye films was also investigated.

Water soluble laser dyes

DOEpatents

Hammond, Peter R.; Feeman, James F.; Field, George F.

1998-01-01

Novel water soluble dyes of the formula I are provided ##STR1## wherein R.sup.1 and R.sup.4 are alkyl of 1 to 4 carbon atoms or hydrogen; or R.sup.1 -R.sup.2 or R.sup.2 -R.sup.4 form part of aliphatic heterocyclic rings; R.sup.2 is hydrogen or joined with R.sup.1 or R.sup.4 as described above; R.sup.3 is --(CH.sub.2).sub.m --SO.sub.3.sup.-, where m is 1 to 6; X is N, CH or ##STR2## where Y is 2 --SO.sub.3.sup.- ; Z is 3, 4, 5 or 6 --SO.sub.3.sup.-. The novel dyes are particularly useful as the active media in water solution dye lasers.

Co2/Erbium:YAG/Dye laser combination: an effective and successful treatment for angiofibromas in tuberous sclerosis.

PubMed

Fioramonti, Paolo; De Santo, Liliana; Ruggieri, Martina; Carella, Sara; Federico, Lo Torto; Onesti, Maria Giuseppina; Scuderi, Nicolò

2014-02-01

Tuberous sclerosis, an autosomal dominant neurocutaneous syndrome seen in approximately 1 in 6,000 people worldwide, is characterized by the appearance of hamartomas in multiple organs. The classic clinical triad consists of angiofibromas, epilepsy, and developmental delay. Dermatologic disorder is one of the main characteristics. Angiofibromas, a common form of presentation, causes significant cosmetic and medical problems. The current treatment for skin lesions is laser therapy. The carbon dioxide (CO2) laser has been used satisfactorily in treating these lesions, but several studies have demonstrated a high percentage of recurrences. Erbium:yttrium-aluminum-garnet (YAG) laser treatment has been used to resurface skin abnormalities in patients with dermatologic conditions. The dye laser as an alternative uses the principles of selective photothermolysis and is very effective in treating the vascular component of tuberous sclerosis. The use of all these lasers to treat skin lesions in patients affected by tuberous sclerosis has never been described in the literature. A retrospective study, conducted from 2007 to May 2013, investigated 13 patients who had tuberous sclerosis treated with an erbium:YAG/CO2/dye laser combination. All the patients showed great improvement of their skin lesions. The results were evident immediately after the first treatment. No patient experienced complications or recurrence. The combined use of the erbium:YAG/Dye/CO2 laser is a safe and effective treatment for skin lesions in patients affected by tuberous sclerosis. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Influence of an Interfacial Effect on the Laser Performance of a Rhodamine 6G/Cellulose Acetate Waveguide on a Vinylidene Fluoride Copolymer Layer.

PubMed

Tsutsumi, Naoto; Hirano, Yoshinori; Kinashi, Kenji; Sakai, Wataru

2018-06-12

The fluorescent properties of dyes and fluorophores in condensed matter significantly affect the laser performance of organic dye lasers and fluorescent polymer lasers. Concentration quenching of fluorescence is commonly observed in condensed matter. Several approaches have been presented to suppress such quenching, such as the use of a dendrimer and the use of effective energy transfer in a guest-host system. The enhanced fluorescence of rhodamine 6G (R6G) dye on a vinylidene fluoride polymer is an alternative method for enhancing laser performance because of the roughness of the P(VDF-TrFE) surface and the interaction between polar β-crystals of P(VDF-TrFE) and R6G dye. In this paper, a significant improvement in slope efficiency (SE) is demonstrated without a significant depression in the lasing threshold for distributed feedback (DFB) and distributed Bragg reflector (DBR) lasers fabricated using an R6G-dispersed cellulose acetate (CA) matrix spin-coated onto a copolymer of vinylidene fluoride and trifluoroethylene P(VDF-TrFE) thin film. SEs of 3.4 and 1.3% were measured for DBR and DFB laser devices with CA/R6G on a P(VDF-TrFE) thin film, respectively, whereas an SE of less than 1.0% was measured for both corresponding laser devices without a P(VDF-TrFE) thin film. From the aspect of simple fabrication procedures, repeatability in device fabrication and performance, stability of the device, time for device fabrication, the present approach is the most preferable way for industrial applications, requiring only the additional step of spin-coating of a P(VDF-TrFE) thin film.

Growth and optical properties of Pr,Yb-codoped KY 3F 10 fluoride single crystals for up-conversion visible luminescence

NASA Astrophysics Data System (ADS)

Kim, Kyoung Jin; Jouini, Anis; Yoshikawa, Akira; Simura, Rayko; Boulon, Georges; Fukuda, Tsuguo

2007-02-01

We investigate different ways to realize laser emission from (Pr 3+) 3P J=0,1,2 levels by pump sources other than the common argon and excimer-dye laser. The use of infrared (IR) laser diodes in combination with intra- and inter-ionic energy transfer processes (up-conversion) could be an efficient solution towards laser oscillation. Pr 3+,Yb 3+-codoped KY 3F 10 (Pr, Yb:KYF) single crystals were successfully grown by the micro-pulling-down (μ-PD) method. The crystals were transparent with a slightly greenish color, 2.0-2.5 mm in diameter, 20-30 mm in length and free from visible inclusions and cracks. Effective segregation coefficients of Pr and Yb in KYF were studied by means of absorption and chemical analysis. Strong visible emission via selective IR pumping with λ=975 nm and up-conversion excitation were obtained in Pr, Yb:KYF at room temperature (RT). Luminescence measurements have been carried out and the decay kinetics of the Pr 3+ visible emissions was investigated by room temperature time-resolved spectra.

Optical properties of cyanine dyes in nanotubes of chrysotile asbestos

NASA Astrophysics Data System (ADS)

Starovoytov, Anton A.; Vartanyan, Tigran A.; Belotitskii, Vladimir I.; Kumzerov, Yuri A.; Sysoeva, Anna A.

2017-08-01

Optical properties of cyanine dye molecules incorporated in nanotubes of natural chrysotile asbestos are studied. The absorption and fluorescence spectra of dye in asbestos have the similar shapes as in the ethanol solution, apart from small blue shift of the maxima. The Stokes shift in asbestos is smaller than in the ethanol solution. The fluorescence decay times of the dyes in asbestos nanotubes are found to be larger than that in the case of thin films of the same dyes formed on the transparent dielectric supports. This observation is rationalized in terms of the stereoisomerization hindrance in the excited electronic state of dye molecules. At the same time linear dichroism and fluorescence anisotropy observed in the experiment indicate that the embedded dye molecules are well-isolated monomer oriented predominantly along asbestos nanotubes.

Femtosecond laser pulse optimization for multiphoton cytometry and control of fluorescence

NASA Astrophysics Data System (ADS)

Tkaczyk, Eric Robert

This body of work encompasses optimization of near infrared femtosecond laser pulses both for enhancement of flow cytometry as well as adaptive pulse shaping to control fluorescence. A two-photon system for in vivo flow cytometry is demonstrated, which allows noninvasive quantification of circulating cell populations in a single live mouse. We monitor fluorescently-labeled red blood cells for more than two weeks, and are also able to noninvasively measure circulation times of two distinct populations of breast cancer cells simultaneously in a single mouse. We build a custom laser excitation source in the form of an extended cavity mode-locked oscillator, which enables superior detection in whole blood or saline of cell lines expressing fluorescent proteins including the green fluorescent protein (GFP), tdTomato and mPlum. A mathematical model explains unique features of the signals. The ability to distinguish different fluorescent species is central to simultaneous measurement of multiple molecular targets in high throughput applications including the multiphoton flow cytometer. We demonstrate that two dyes which are not distinguishable to one-photon measurements can be differentiated and in fact quantified in mixture via phase-shaped two-photon excitation pulses found by a genetic algorithm. We also selectively enhance or suppress two-photon fluorescence of numerous common dyes with tailored pulse shapes. Using a multiplicative (rather than ratiometric) fitness parameter, we are able to control the fluorescence while maintaining a strong signal. With this method, we control the two-photon fluorescence of the blue fluorescent protein (BFP), which is of particular interest in investigations of protein-protein interactions, and has frustrated previous attempts of control. Implementing an acousto-optic interferometer, we use the same experimental setup to measure two-photon excitation cross-sections of dyes and prove that photon-photon interferences are the predominant mechanism of control. This research establishes the basis for molecularly tailored pulse shaping in multiphoton flow cytometry, which will advance our ability to probe the biology of circulating cells during disease progression and response to therapy.

Tuned alexandrite laser for dentistry

NASA Astrophysics Data System (ADS)

Sulc, Jan; Jelinkova, Helena; Dostalova, Tatjana; Miyagi, Mitsunobu

2000-04-01

For medical applications tunable solid state laser system emitting a visible light in a region from 710 to 775 nm was developed. Laser head of this system was composed of alexandrite crystal rod (4 mm in diameter, 90 mm of length), two dielectric mirrors and tuning element (dispersing prism or thin-film polarizer). For Q-switching, three different optics shutters were proved: a saturable NC-dye or LiF crystal, LiNbO3 Pockels cell, and rotating prism. Maximum output energy in free-running and Q-switching regime was 400 mJ and 70 mJ, respectively. The pulse duration was 70 microsecond(s) in the free-running and 45 - 250 ns in Q-switching regime. The output free running laser radiation was guided via a multimode quartz fiber (1 m of length, core diameter 600 micrometers ) or via a special fluorocarbon polymer-coated silver hollow glass waveguide (0.62 m of length, inner diameter 1 mm). At first, this radiation with a laser fluence of 2.5 J/cm2 was used for an ablation of a dental calculus. Next, the laser radiation propagation in a root canal and its effect on bacteria was proved. The dissipated energy measurement was made inside and outside of the tooth. From the results follow that the alexandrite laser could be useful for medical applications.

PHYSICAL EFFECTS OCCURRING DURING GENERATION AND AMPLIFICATION OF LASER RADIATION: Enhancement of the efficiency of flashlamp-pumped lasers by conversion of the spectral composition of the exciting radiation

NASA Astrophysics Data System (ADS)

Levin, M. B.; Cherkasov, A. S.

1989-02-01

An account is given of the published investigations of ways of increasing the efficiency of flashlamp-pumped lasers by frequency conversion of the exciting radiation with the aid of luminescent filters. An analysis is made of the method for calculating the efficiency of luminescent filters absorbing short-wavelength radiation and reemitting it in the absorption region of the active medium. It is shown that the use of rhodamine 6G and other phosphors as luminescent filters can double the efficiency of neodymium glass lasers, increase the efficiency of YAG:Nd3+ lasers by a factor of 1.5, and improve the efficiency of lasers activated with Ti3+ by more than an order of magnitude. The use of luminescent filters in dye lasers can double the efficiency and make it possible to reach average output powers of hundreds of watts. Promising materials for luminescent filters are considered and margins for increasing their efficiency are analyzed. The main results are reported of studies of plasma pump-spectrum converters and it is shown that promising results can be expected by combining luminescent filters and an optimized plasma converter system in an "optical boiler" enclosure.

Estimation of ground and excited state dipole moment of laser dyes C504T and C521T using solvatochromic shifts of absorption and fluorescence spectra.

PubMed

Basavaraja, Jana; Suresh Kumar, H M; Inamdar, S R; Wari, M N

2016-02-05

The absorption and fluorescence spectra of laser dyes: coumarin 504T (C504T) and coumarin 521T (C521T) have been recorded at room temperature in a series of non-polar and polar solvents. The spectra of these dyes showed bathochromic shift with increasing in solvent polarity indicating the involvement of π→π⁎ transition. Kamlet-Taft and Catalan solvent parameters were used to analyze the effect of solvents on C504T and C521T molecules. The study reveals that both general solute-solvent interactions and specific interactions are operative in these two systems. The ground state dipole moment was estimated using Guggenheim's method and also by quantum mechanical calculations. The solvatochromic data were used to determine the excited state dipole moment (μ(e)). It is observed that dipole moment value of excited state (μ(e)) is higher than that of the ground state in both the laser dyes indicating that these dyes are more polar in nature in the excited state than in the ground state. Copyright © 2015. Published by Elsevier B.V.

Optical properties and emissivities of liquid metals and alloys

NASA Technical Reports Server (NTRS)

Krishnan, Shankar; Nordine, Paul C.

1993-01-01

This paper presents the results from our on-going program to investigate the optical properties of liquid metals and alloys at elevated temperatures. Ellipsometric and polarimetric techniques have been used to investigate the optical properties of materials in the 1000 - 3000 K temperature range and in the 0.3 - 0.1 mu m wavelength range. The ellipsometric and polarimetric techniques are described and the characteristics of the instruments are presented. The measurements are conducted by reflecting a polarized laser beam from an electromagnetically levitated liquid metal or alloy specimen. A Rotating Analyzer Ellipsometer (RAE) or a four-detector Division-of-Amplitude Photopolarimeter (DOAP) is used to determine the polarimetric properties of the light reflected at an angle of incidence of approximately 68 deg. Optical properties of the specimen which are calculated from these measurements include the index of refraction, extinction coefficient, normal spectral emissivity, and spectral hemispherical emissivity. These properties have been determined at various wavelengths and temperatures for liquid Ag, Al, Au, Cu, Nb, Ni, Pd, Pt, Si, Ti, Ti-Al alloys, U, and Zr. We also describe new experiments using pulsed-dye laser spectroscopic ellipsometry for studies of the wavelength dependence of the emissivities and optical properties of materials at high temperature. Preliminary results are given for liquid Al. The application of four-detector polarimetry for rapid determination of surface emissivity and true temperature is also described. Characteristics of these devices are presented. An example of the accuracy of this instrument in measurements of the melting point of zirconium is illustrated.

Dynamics of excimer laser ablation of thin tungsten films studied by fast photography

NASA Astrophysics Data System (ADS)

Toth, Zsolt; Hopp, Bela; Kantor, Zoltan; Ignacz, Ferenc; Szoerenyi, Tamas; Bor, Zsolt

1994-09-01

The time evolution of ablation and material transport during ArF excimer laser induced blow off of tungsten films from glass substrates is studied by fast photography using delayed dye laser pulses. The analysis of experimental results combined with heat flow calculations provides evidence that tungsten removal in the solid phase is the dominant mechanism in the 40 - 200 mJ/cm2 fluence domain, while partially inhomogeneous melting is observed between 200 and 800 mJ/cm2. In this fluence range, solid fragments and a halo consisting of molten droplets are observed indicating spatial separation of the two phases. The molten phase advances faster, forming a protective mist in front of the solid piece(s). At yet higher fluences (800 - 1000 mJ/cm2), a well separated solid phase could be recorded under the halo although model calculations suggest full vaporization of the layer. This unexpected phenomenon is explained by the optical shielding effect of the halo.

Balloon borne in-situ detection of OH in the stratosphere from 37 to 23 km

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stimpfle, R.M.; Lapson, L.B., Wennberg, P.O.; Anderson, J.G.

1989-12-01

The OH number density in the stratosphere has been measured over the altitude interval of 37 to 23 km at midday via balloon-borne gondola launched from Palestine, Texas on July 6, 1988. OH radicals are detected with a laser induced fluorescence instrument employing a 17 kHz repetition rate copper vapor laser pumped dye laser optically coupled to an enclosed flow, in-situ sampling chamber. OH abundances ranged from 88 {plus minus} 31 pptv (1.1 {plus minus} 0.4 {times} 10{sup 7} molec cm{sup {minus}3}) in the 36 to 35 km interval to 0.9 {plus minus} 0.8 pptv (8.7 {plus minus} 7.7 {times}10{supmore » 5} molec cm{sup {minus}3}) in the 24 to 23 km interval. The stated uncertainty ({plus minus} 1{sigma}) includes that from both measurement precision and accuracy. Simultaneous detection of ozone and water vapor densities was carried out with separate on-board instruments.« less

Strong coupling between surface plasmon polariton and laser dye rhodamine 800

NASA Astrophysics Data System (ADS)

Valmorra, Federico; Bröll, Markus; Schwaiger, Stephan; Welzel, Nadine; Heitmann, Detlef; Mendach, Stefan

2011-08-01

We report on strong coupling between surface plasmon polaritons on a thin silver film and laser dye Rhodamine 800. Attenuated total reflection measurements reveal that the pure surface plasmon polaritons interact with the Rhodamine 800 absorption lines exhibiting pronounced anticrossings in the dispersion relation. We show that the corresponding energy gap can be tailored by the concentration of dye molecules in the dielectric matrix between 50 meV and 70 meV. We can well model our data by a classical transfer matrix approach as well as by a quantum mechanical coupled oscillator ansatz.

Laser selective microablation of sensitized intracellular components within auditory receptor cells

NASA Astrophysics Data System (ADS)

Harris, David M.; Evans, Burt N.; Santos-Sacchi, Joseph

1995-05-01

A laser system can be coupled to a light microscope for laser microbeam ablation and trapping of single cells in vitro. We have extended this technology by sensitization of target structures with vital dyes to provide selective ablation of specific subcellular components. Isolated auditory receptor cells (outer hair cells, OHCs) are known to elongate and contract in response to electrical, chemical and mechanical stimulation. Various intracellular structures are candidate components mediating motility of OHCs, but the exact mechanism(s) is currently unknown. In ongoing studies of OHC motility, we have used the microbeam for selective ablation of lateral wall components and of an axial cytoskeletal core that extends from the nucleus to the cell apex. Both the area beneath the subsurface cistemae of the lateral wall and the core are rich in mitochondria. OHCs isolated from guinea pig cochlea are suspended in L- 15 medium containing 2.0 (mu) M Rhodamine 123, a porphyrin with an affinity for mitochondria. A spark-pumped nitrogen laser pumping a dye cell (Coumarin 500) was aligned on the optical axis of a Nikon Optiphot-2 to produce a 3 ns, 0.5 - 10 micrometers spot (diameter above ablation threshold w/50X water immersion, N.A. 0.8), and energy at the target approximately equals 10 (mu) J/pulse. At short incubation times in Rh123 irradiation caused local blebbing or bulging of cytoplastic membrane and thus loss of the OHC's cylindrical shape. At longer Rh123 incubation times when the central axis of the cell was targeted we observed cytoplasmic clearing, immediate cell elongation (approximately equals 5%) and clumping of core material at nuclear and apical attachments. Experiments are underway to examine the significance of these preliminary observations.

Optical properties of voltage sensitive hemicyanine dyes of variable hydrophobicity confined within surfactant micelles

NASA Astrophysics Data System (ADS)

Naeem, Kashif; Naseem, Bushra; Shah, S. S.; Shah, Syed W. H.

2017-11-01

The optical properties of amphiphilic hemicyanine dyes with variable hydrophobicity, confined within anionic micelles of sodium dodecylbenzenesulfonate (NaDDBS) have been studied by UV-visible absorption spectroscopy. The confinement constant, K conf has been determined for each entrapped dye. The ion-pair formation between dye and surfactant causes a decline in electronic transition energy (ΔE T) when dye alkyl chains are smaller due to stabilization of both the ground and excited state. ΔE T values gradually increase with increase in dye hydrophobicity that hampers the electrostatic interaction with dialkylammonium moiety and consequently excited state stabilization is compromised. The average number of dye molecules trapped in a single micelle was also determined. The negative values of Gibbs free energy indicate that the dye entrapment within micelles is energetically favored. These findings have significance for developing functional materials with peculiar luminescent properties, especially for more effective probing of complex biological systems.

Optical properties of anthocyanin dyes on TiO2 as photosensitizers for application of dye-sensitized solar cell (DSSC)

NASA Astrophysics Data System (ADS)

Ahliha, A. H.; Nurosyid, F.; Supriyanto, A.; Kusumaningsih, T.

2018-03-01

Dye-sensitized solar cell (DSSC) is one of the alternative energy that can convert light energy into electrical energy. The component of DSSC consists of FTO substrates, TiO2, electrolyte, dye sensitizer, and counter electrode. This study aim was to determine the effect of optical properties of anthocyanin dyes on efficiency of DSSC. The dye sensitizer used can be extracted from anthocyanin pigments such as dragon fruit, black rice, and red cabbage. The red cabbage sensitizer shows lower absorbance value in the visible range (450-580 nm), than dragon fruit and black rice. The chemical structure of each dye molecules has an R group (carbonyl and hydroxyl) that forms a bond with the oxide layer. Red cabbage dye cell has the highest efficiency, 0.06% then dragon fruit and black rice, 0.02% and 0.03%.

Construction and use of a zebrafish heart voltage and calcium optical mapping system, with integrated electrocardiogram and programmable electrical stimulation

PubMed Central

Lin, Eric; Craig, Calvin; Lamothe, Marcel; Sarunic, Marinko V.; Beg, Mirza Faisal

2015-01-01

Zebrafish are increasingly being used as a model of vertebrate cardiology due to mammalian-like cardiac properties in many respects. The size and fecundity of zebrafish make them suitable for large-scale genetic and pharmacological screening. In larger mammalian hearts, optical mapping is often used to investigate the interplay between voltage and calcium dynamics and to investigate their respective roles in arrhythmogenesis. This report outlines the construction of an optical mapping system for use with zebrafish hearts, using the voltage-sensitive dye RH 237 and the calcium indicator dye Rhod-2 using two industrial-level CCD cameras. With the use of economical cameras and a common 532-nm diode laser for excitation, the rate dependence of voltage and calcium dynamics within the atrial and ventricular compartments can be simultaneously determined. At 140 beats/min, the atrial action potential duration was 36 ms and the transient duration was 53 ms. With the use of a programmable electrical stimulator, a shallow rate dependence of 3 and 4 ms per 100 beats/min was observed, respectively. In the ventricle the action potential duration was 109 ms and the transient duration was 124 ms, with a steeper rate dependence of 12 and 16 ms per 100 beats/min. Synchronous electrocardiograms and optical mapping recordings were recorded, in which the P-wave aligns with the atrial voltage peak and R-wave aligns with the ventricular peak. A simple optical pathway and imaging chamber are detailed along with schematics for the in-house construction of the electrocardiogram amplifier and electrical stimulator. Laboratory procedures necessary for zebrafish heart isolation, cannulation, and loading are also presented. PMID:25740339

Development of Novel Composite and Random Materials for Nonlinear Optics and Lasers

NASA Technical Reports Server (NTRS)

Noginov, Mikhail

2002-01-01

A qualitative model explaining sharp spectral peaks in emission of solid-state random laser materials with broad-band gain is proposed. The suggested mechanism of coherent emission relies on synchronization of phases in an ensemble of emitting centers, via time delays provided by a network of random scatterers, and amplification of spontaneous emission that supports the spontaneously organized coherent state. Laser-like emission from powders of solid-state luminophosphors, characterized by dramatic narrowing of the emission spectrum and shortening of emission pulses above the threshold, was first observed by Markushev et al. and further studied by a number of research groups. In particular, it has been shown that when the pumping energy significantly exceeds the threshold, one or several narrow emission lines can be observed in broad-band gain media with scatterers, such as films of ZnO nanoparticles, films of pi-conjugated polymers or infiltrated opals. The experimental features, commonly observed in various solid-state random laser materials characterized by different particle sizes, different values of the photon mean free path l*, different indexes of refraction, etc.. can be described as follows. (Liquid dye random lasers are not discussed here.)

Feasibility study: Monodisperse polymer particles containing laser-excitable dyes

NASA Technical Reports Server (NTRS)

Venkateswarlu, Putcha; He, K. X.; Sharma, A.

1993-01-01

The optical properties associated with small particles, which include aerosols, hydrosols and solid microspheres have an impact on several areas of science and engineering. Since the advent of high-speed computers and lasers, the interaction of light with matter in the form of small particles with a discontinuous optical boundary relative to the surroundings has been much better understood. Various nonlinear optical effects have been observed involving interaction of a laser beam with both solid microspheres and liquid microdroplets. These include observation of second and third harmonic generation, four wave mixing, optical visibility, two photon absorption, observation of stimulated emission and lasing, and Stimulated Raman Scattering. Many of these effects are observed with laser intensities which are orders of magnitude less than that required by threshold condition for interactions in macroscopic bulk medium. The primary reason for this is twofold. The front surface of the microsphere acts as a thick lens to enhance the internal intensity of the input laser radiation, and the spherical shape of the droplet acts as an optical cavity to provide feedback at specific wavelengths corresponding to the whispering gallery modes or the morphology dependent resonances (MDR's). The most interesting and significant recent finding in this field is undoubtedly the existence of resonance peaks in linear and nonlinear optical spectra. Such resonance peaks are only dependent on the particle morphology, which means the size, shape and refractive index of the particle. Because of the simultaneous presence of these resonances, they have been referred to by many names, including structural resonances, whispering modes or whispering gallery modes, creeping waves, circumferential waves, surfaces modes, and virtual modes. All of these names refer to the same phenomena, i.e. morphology dependent resonances (MDR's) which has already been described and predicted precisely by electromagnetic theory and Loentz-Mie theory since 1908. MDR's can become important when the particle size (radius a) approaches and exceeds the wavelength of the electromagnetic wave (lambda) and the refractive index of the particle is greater than that of the surrounding medium. Such resonances are easiest to observe from a single particle with high symmetry, such as a sphere, spheroid, or cylinder. MDR's correspond to solutions of the characteristic equations of the electromagnetic fields in the presence of a sphere.

IV INTERNATIONAL CONFERENCE ON ATOM AND MOLECULAR PULSED LASERS (AMPL'99): Radiative and photochemical properties of organic compounds excited by high-power XeCl laser radiation

NASA Astrophysics Data System (ADS)

Kopylova, T. N.; Kuznetsova, Rimma T.; Svetlichnyi, Valerii A.; Sergeev, A. K.; Tel'minov, E. N.; Filinov, D. N.

2000-06-01

Radiative and photochemical properties of a number of laser dyes excited by focused radiation of a XeCl laser with intensity up to 200 MW cm-2 were studied. A method for measuring the gain of organic molecules under high-power excitation is proposed. The dependence of the dye transmittance for the pump radiation on its intensity was studied. It is shown that changes in energy, spectral, and time characteristics of radiation and the photostability of compounds under high-power excitation are associated with the formation of superluminescence.

Comparison of the effect of the carbon dioxide laser and the bipolar coagulator on the cat brain

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cozzens, J.W.; Cerullo, L.J.

The carbon dioxide laser has recently received clinical acceptance in neurosurgical practice. There are, however, few studies reported in the neurosurgical literature, either clinical or experimental, concerning its safety or efficacy on a physiological level by comparison to a more conventional tool. This study is not a description of a surgical technique, but is rather a basic physiological comparison of two surgical instruments. In this study, 11 cats were pretreated with the protein-bound dye, Evans blue. A corticotomy was performed in one hemisphere with the carbon dioxide laser and in the other with a microbipolar coagulator and a sharp blade.more » The subsequent extravasation of dye was presumed to be proportional to the amount of blood-brain barrier disruption associated with each lesion. When effective power settings for the two devices were compared, the laser lesions had significantly less extravasation of blue dye. This indicated that there was less damage to the blood-brain barrier surrounding laser corticotomy than surrounding conventional bipolar coagulation and sharp dissection at comparable power settings for each modality.« less

Laser velocimetry with fluorescent dye-doped polystyrene microspheres.

PubMed

Lowe, K Todd; Maisto, Pietro; Byun, Gwibo; Simpson, Roger L; Verkamp, Max; Danehy, Paul M; Tiemsin, Pacita I; Wohl, Christopher J

2013-04-15

Simultaneous Mie scattering and laser-induced fluorescence (LIF) signals are obtained from individual polystyrene latex microspheres dispersed in an air flow. Microspheres less than 1 μm mean diameter were doped with two organic fluorescent dyes, Rhodamine B (RhB) and dichlorofluorescein (DCF), intended either to provide improved particle-based flow velocimetry in the vicinity of surfaces or to provide scalar flow information (e.g., marking one of two fluid streams). Both dyes exhibit measureable fluorescence signals that are on the order of 10(-3) to 10(-4) times weaker than the simultaneously measured Mie signals. It is determined that at the conditions measured, 95.5% of RhB LIF signals and 32.2% of DCF signals provide valid laser-Doppler velocimetry measurements compared with the Mie scattering validation rate with 6.5 W of 532 nm excitation, while RhB excited with 1.0 W incident laser power still exhibits 95.4% valid velocimetry signals from the LIF channel. The results suggest that the method is applicable to wind tunnel measurements near walls where laser flare can be a limiting factor and monodisperse particles are essential.

Diagnosis of NMOS DRAM functional performance as affected by a picosecond dye laser

NASA Technical Reports Server (NTRS)

Kim, Q.; Schwartz, H. R.; Edmonds, L. D.; Zoutendyk, J. A.

1992-01-01

A picosec pulsed dye laser beam was at selected wavelengths successfully used to simulate heavy-ion single-event effects (SEEs) in negative channel NMOS DRAMs. A DRAM was used to develop the test technique because bit-mapping capability and previous heavy-ion upset data were available. The present analysis is the first to establish such a correlation between laser and heavy-ion data for devices, such as the NMOS DRAM, where charge collection is dominated by long-range diffusion, which is controlled by carrier density at remote distances from a depletion region. In the latter case, penetration depth is an important parameter and is included in the present analysis. A single-pulse picosecond dye laser beam (1.5 microns diameter) focused onto a single cell component can upset a single memory cell; clusters of memory cell upsets (multiple errors) were observed when the laser energy was increased above the threshold energy. The multiple errors were analyzed as a function of the bias voltage and total energy of a single pulse. A diffusion model to distinguish the multiple upsets from the laser-induced charge agreed well with previously reported heavy ion data.

Dye laser traveling wave amplifier

NASA Technical Reports Server (NTRS)

Davidson, F.; Hohman, J.

1984-01-01

A flashlamp pumped dye laser suitable for use as a single stage amplifier is described. Particular emphasis is placed on the efforts to increase output pulse energy and improve the temporal profile of the injected pulse. By using high power thin film polarizers, output energies reach from 4 to 45 mJ. Various dispersive elements are used to develop an amplified pulse with an extremely clean temporal profile.

Optical and morphological characterizations of pyronin dye-poly (vinyl alcohol) thin films formed on glass substrates

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meral, Kadem, E-mail: kademm@atauni.edu.tr; Arik, Mustafa, E-mail: marik@tatauni.edu.tr; Onganer, Yavuz, E-mail: yonganer@atauni.edu.tr

Thin films of pyronin dye mixed with poly(vinyl alcohol) (PVA) on glass substrate were prepared by using spin-coating technique. The optical and morphological properties of the thin films were studied by UV-Vis., steady-state fluorescence spectroscopies and atomic force microscopy (AFM). The thin films on glass substrate were fabricated at various [PVA]/[dye] (P/D) ratios. Hence, the monomeric and H-aggregates thin films of pyronin dye mixed with PVA were formed as a function of the dye and PVA concentration. It was determined that while the monomeric thin films showed strong fluorescence, the formation of H-aggregates in the thin film caused to decreasingmore » the fluorescence intensity. AFM studies demonstrated that the morphology of the thin film was drastically varied with changing the optical property of the thin film such as monomeric and H-aggregates thin films.« less

Large electronic third-order optical nonlinearities of cyanine dyes measured by resonant femtosecond degenerate four-wave mixing

NASA Astrophysics Data System (ADS)

Kasatani, Kazuo

2003-01-01

Third-order optical nonlinearities of several cyanine dyes were measured under resonant conditions by the femtosecond degenerate four-wave mixing (DFWM) technique. Temporal profiles of the DFWM signal were measured with a time resolution of 0.3 ps, and were found to consist of at least two components, the coherent instantaneous nonlinear response and the delayed response with a decay time constant of several hundred picoseconds. The latter can be attributed to molecular rotational relaxation of these dyes. The values of electronic component of the optical nonlinear susceptibility, χ e xxxx (3), for these dyes were ≈2×10 -12 esu at the very low concentration of 1×10 -5 mol dm -3. The electronic component of molecular hyperpolarizability, γe, was calculated to be ≈1×10 -28 esu for each dye.

Development of New Laser-Protective Dyes. Phase 1.

DTIC Science & Technology

1990-10-30

technology to stabilize cyanine and squarylium dyes . This accomplishment will justify continued research on the synthesis and process development of...beyond. This is the subject of a proposed Phase II program. RESULTS AND DISCUSSION THERMAL STABILITY: In Phase I, dyes of the cyanine and squarylium ...Test in Appendix 1). Table 1 shows that the squarylium dyes are inherently more thermally stable than the cyanines. This observation supports

Molecular engineering of fluorescein dyes as complementary absorbers in dye co-sensitized solar cells

DOE PAGES

Pepe, Giulio; Cole, Jacqueline M.; Waddell, Paul G.; ...

2016-09-22

Fluorescein dye derivatives exhibit extended optical absorption up to 500 nm, rendering these compounds suitable as co-absorbers in dye-sensitized solar cells (DSCs). A molecular engineering approach is presented, which embraces this intrinsic optical attribute of fluoresceins, while modifying the dye chemistry to enhance their light harvesting efficiency, in order to effectively tailor them for DSC applications. This approach first realizes relationships between the molecular structure and the optoelectronic properties for a series of five a priori known (parent) fluorescein dyes: 5-carboxyfluorescein (1), a mixture of m-carboxyfluorescein where m = 5 or 6 (2), 5-carboxyfluorescein diacetate (3), 6-carboxyfluorescein diacetate (4), amore » mixture of n-carboxy-2',7'-dichlorofluorescein diacetate where n = 5 or 6 (5). The first step in this approach combines, where available, experimental and computational methods so that electronic structure calculations can also be validated for representative fluorescein dyes. Such calculations can then be used reliably to predict the structure and properties of fluorescein dyes for cases where experimental data are lacking. Structure-function relationships established from this initial step inform the selection of parent dye 1 that is taken forward to the second step in molecular engineering: in silico chemical derivation to re-functionalize 1 for DSC applications. For this purpose, computational calculations are used to extend the charge conjugation in 1 between its donor and acceptor moieties. These structural modifications result in a bathochromic shift of the lowest excitation by ~1.3-1.9 eV (100-170 nm), making the dye optically absorb in the visible region. Further calculations on dye molecules adsorbed onto the surface of a TiO 2 cluster are used to investigate the dye sensitization behavior via dye adsorption energies and anchoring modes. The results of this theoretical investigation lead to two molecularly engineered fluoresceins being proposed to act as co-sensitizers together with a rhodamine dye. This combination of three dyes ensures chemical compatibility, panchromatic absorption, and restores optical absorption dipping otherwise observed in a DSC device at ~350-400 nm owing to the I-/I- 3 electrolyte. Altogether, the results of this study demonstrate that molecular engineering can be used to identify suitable chemical modifications for organic dyes with improved light harvesting properties for photovoltaic applications.« less

Molecular engineering of fluorescein dyes as complementary absorbers in dye co-sensitized solar cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pepe, Giulio; Cole, Jacqueline M.; Waddell, Paul G.

Fluorescein dye derivatives exhibit extended optical absorption up to 500 nm, rendering these compounds suitable as co-absorbers in dye-sensitized solar cells (DSCs). A molecular engineering approach is presented, which embraces this intrinsic optical attribute of fluoresceins, while modifying the dye chemistry to enhance their light harvesting efficiency, in order to effectively tailor them for DSC applications. This approach first realizes relationships between the molecular structure and the optoelectronic properties for a series of five a priori known (parent) fluorescein dyes: 5-carboxyfluorescein (1), a mixture of m-carboxyfluorescein where m = 5 or 6 (2), 5-carboxyfluorescein diacetate (3), 6-carboxyfluorescein diacetate (4), amore » mixture of n-carboxy-2',7'-dichlorofluorescein diacetate where n = 5 or 6 (5). The first step in this approach combines, where available, experimental and computational methods so that electronic structure calculations can also be validated for representative fluorescein dyes. Such calculations can then be used reliably to predict the structure and properties of fluorescein dyes for cases where experimental data are lacking. Structure-function relationships established from this initial step inform the selection of parent dye 1 that is taken forward to the second step in molecular engineering: in silico chemical derivation to re-functionalize 1 for DSC applications. For this purpose, computational calculations are used to extend the charge conjugation in 1 between its donor and acceptor moieties. These structural modifications result in a bathochromic shift of the lowest excitation by ~1.3-1.9 eV (100-170 nm), making the dye optically absorb in the visible region. Further calculations on dye molecules adsorbed onto the surface of a TiO 2 cluster are used to investigate the dye sensitization behavior via dye adsorption energies and anchoring modes. The results of this theoretical investigation lead to two molecularly engineered fluoresceins being proposed to act as co-sensitizers together with a rhodamine dye. This combination of three dyes ensures chemical compatibility, panchromatic absorption, and restores optical absorption dipping otherwise observed in a DSC device at ~350-400 nm owing to the I-/I- 3 electrolyte. Altogether, the results of this study demonstrate that molecular engineering can be used to identify suitable chemical modifications for organic dyes with improved light harvesting properties for photovoltaic applications.« less

Masked red-emitting carbopyronine dyes with photosensitive 2-diazo-1-indanone caging group.

PubMed

Kolmakov, Kirill; Wurm, Christian; Sednev, Maksim V; Bossi, Mariano L; Belov, Vladimir N; Hell, Stefan W

2012-03-01

Caged near-IR emitting fluorescent dyes are in high demand in optical microscopy but up to now were unavailable. We discovered that the combination of a carbopyronine dye core and a photosensitive 2-diazo-1-indanone residue leads to masked near-IR emitting fluorescent dyes. Illumination of these caged dyes with either UV or visible light (λ < 420 nm) efficiently generates fluorescent compounds with absorption and emission at 635 nm and 660 nm, respectively. A high-yielding synthetic route with attractive possibilities for further dye design is described in detail. Good photostability, high contrast, and a large fluorescence quantum yield after uncaging are the most important features of the new compounds for non-invasive imaging in high-resolution optical microscopy. For use in immunolabelling the caged dyes were decorated with a (hydrophilic) linker and an (activated) carboxyl group.

MHI-148 Cyanine Dye Conjugated Chitosan Nanomicelle with NIR Light-Trigger Release Property as Cancer Targeting Theranostic Agent.

PubMed

Thomas, Reju George; Moon, Myeong Ju; Surendran, Suchithra Poilil; Park, Hyeong Ju; Park, In-Kyu; Lee, Byeong-Il; Jeong, Yong Yeon

2018-02-15

Paclitaxel (PTX) loaded hydrophobically modified glycol chitosan (HGC) micelle is biocompatible in nature, but it requires cancer targeting ability and stimuli release property for better efficiency. To improve tumor retention and drug release characteristic of HGC-PTX nanomicelles, we conjugated cancer targeting heptamethine dye, MHI-148, which acts as an optical imaging agent, targeting moiety and also trigger on-demand drug release on application of NIR 808 nm laser. The amine group of glycol chitosan modified with hydrophobic 5β-cholanic acid and the carboxyl group of MHI-148 were bonded by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide chemistry. Paclitaxel was loaded to MHI-HGC nanomicelle by an oil-in-water emulsion method, thereby forming MHI-HGC-PTX. Comparison of near infrared (NIR) dyes, MHI-148, and Flamma-774 conjugated to HGC showed higher accumulation for MHI-HGC in 4T1 tumor and 4T1 tumor spheroid. In vitro studies showed high accumulation of MHI-HGC-PTX in 4T1 and SCC7 cancer cell lines compared to NIH3T3 cell line. In vivo fluorescence imaging of the 4T1 and SCC7 tumor showed peak accumulation of MHI-HGC-PTX at day 1 and elimination from the body at day 6. MHI-HGC-PTX showed good photothermal heating ability (50.3 °C), even at a low concentration of 33 μg/ml in 1 W/cm 2 808 nm laser at 1 min time point. Tumor reduction studies in BALB/c nude mice with SCC7 tumor showed marked reduction in MHI-HGC-PTX in the PTT group combined with photothermal therapy compared to MHI-HGC-PTX in the group without PTT. MHI-HGC-PTX is a cancer theranostic agent with cancer targeting and optical imaging capability. Our studies also showed that it has cancer targeting property independent of tumor type and tumor reduction property by combined photothermal and chemotherapeutic effects.

Long pulsed dye laser treatment of facial wrinkles.

PubMed

Tay, Yong-Kwang; Khoo, Boo-Peng; Tan, Eileen; Kwok, Colin

2004-11-01

The flashlamp pulsed dye laser has been used in the treatment of rhytids. To evaluate the efficacy of the long pulsed dye laser in the treatment of mild to moderate wrinkles in Asian patients. Wrinkles on one half of the face in 10 subjects were treated with the long pulsed dye laser (595 nm, 10 mm spot size, 10 ms, 7 J/cm2, 40 ms spray, 40 ms delay, single-pass, 30% overlap) with the other side serving as a control. A total of three treatments were given at 2 monthly intervals. The following sites were treated: periorbital area, six patients; forehead, two patients; cheek, two patients. No preoperative anesthesia or postoperative treatment were used. Clinical photographs were taken before and after each treatment, and analysis was undertaken through photographic evaluation by non-treating physicians. At 2 months after the last treatment, the clinical improvement of rhytids was noted in all patients compared with baseline. Four subjects had mild improvement (< or = 25%), five had moderate improvement (26-50%) and one had marked improvement (51-75%). The periorbital area was more responsive to treatment compared with the other sites. No clinical changes were noted in the control areas. No adverse effects were reported except for transient mild erythema in two patients which lasted for up to an hour. Nine patients were somewhat satisfied with the treatment and one was highly satisfied. All wanted the other half of the face to be treated. Treatment with a non-ablative 595 nm flashlamp pulsed dye laser can lead to mild to moderate clinical improvement in class I-II rhytids with minimal to no side effects in patients with darker skin types.

Water soluble laser dyes

DOEpatents

Hammond, P.R.; Feeman, J.F.; Field, G.F.

1998-08-11

Novel water soluble dyes of the formula 1 are provided by the formula described in the paper wherein R{sup 1} and R{sup 4} are alkyl of 1 to 4 carbon atoms or hydrogen; or R{sup 1}--R{sup 2} or R{sup 2}--R{sup 4} form part of aliphatic heterocyclic rings; R{sup 2} is hydrogen or joined with R{sup 1} or R{sup 4} as described above; R{sup 3} is --(CH{sub 2}){sub m}--SO{sub 3}{sup {minus}}, where m is 1 to 6; X is N, CH or formula 2 given in paper where Y is 2 --SO{sub 3}{sup {minus}} ; Z is 3, 4, 5 or 6 --SO{sub 3}{sup {minus}}. The novel dyes are particularly useful as the active media in water solution dye lasers.

LASER APPLICATIONS AND OTHER TOPICS IN QUANTUM ELECTRONICS: Influence of the layer thickness and concentration of dye molecules on the emission amplification in cholesteric liquid crystals

NASA Astrophysics Data System (ADS)

Alaverdyan, R. B.; Gevorgyan, A. A.; Chilingaryan, A. D.; Chilingaryan, Yu S.

2008-05-01

The propagation of light through a planar layer of a cholesteric liquid crystal doped with dye molecules is considered. The features of the emission spectra of the crystal are studied both in the absence and presence of dielectric boundaries. The increase in the emission intensity is investigated for different layer thicknesses and different concentrations of dye molecules. It is shown that an anomalously strong increase in the emission intensity with the diffraction intrinsic polarisation takes place in the case of a comparatively small crystal thickness and a relatively low concentration of dye molecules. The obtained results can be used for the development of miniature lasers with the circular polarisation of the fundamental radiation mode.

Thermo-optical interactions in a dye-microcavity photon Bose-Einstein condensate

NASA Astrophysics Data System (ADS)

Alaeian, Hadiseh; Schedensack, Mira; Bartels, Clara; Peterseim, Daniel; Weitz, Martin

2017-11-01

Superfluidity and Bose-Einstein condensation are usually considered as two closely related phenomena. Indeed, in most macroscopic quantum systems, like liquid helium, ultracold atomic Bose gases, and exciton-polaritons, condensation and superfluidity occur in parallel. In photon Bose-Einstein condensates realized in the dye microcavity system, thermalization does not occur by direct interaction of the condensate particles as in the above described systems, i.e. photon-photon interactions, but by absorption and re-emission processes on the dye molecules, which act as a heat reservoir. Currently, there is no experimental evidence for superfluidity in the dye microcavity system, though effective photon interactions have been observed from thermo-optic effects in the dye medium. In this work, we theoretically investigate the implications of effective thermo-optic photon interactions, a temporally delayed and spatially non-local effect, on the photon condensate, and derive the resulting Bogoliubov excitation spectrum. The calculations suggest a linear photon dispersion at low momenta, fulfilling the Landau’s criterion of superfluidity. We envision that the temporally delayed and long-range nature of the thermo-optic photon interaction offer perspectives for novel quantum fluid phenomena.

Tunable organic distributed feedback dye laser device excited through Förster mechanism

NASA Astrophysics Data System (ADS)

Tsutsumi, Naoto; Hinode, Taiki

2017-03-01

Tunable organic distributed feedback (DFB) dye laser performances are re-investigated and characterized. The slab-type waveguide DFB device consists of air/active layer/glass substrate. Active layer consisted of tris(8-quinolinolato)aluminum (Alq3), 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM) dye, and polystyrene (PS) matrix. Effective energy transfer from Alq3 to DCM through Förster mechanism enhances the laser emission. Slope efficiency in the range of 4.9 and 10% is observed at pump energy region higher than 0.10-0.15 mJ cm-2 (lower threshold), which is due to the amplified spontaneous emission (ASE) and lasing. Typical slope efficiency for lasing in the range of 2.0 and 3.0% is observed at pump energy region higher than 0.25-0.30 mJ cm-2 (higher threshold). The tuning wavelength for the laser emission is ranged from 620 to 645 nm depending on the ASE region.

Color matters--material ejection and ion yields in UV-MALDI mass spectrometry as a function of laser wavelength and laser fluence.

PubMed

Soltwisch, Jens; Jaskolla, Thorsten W; Dreisewerd, Klaus

2013-10-01

The success of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) as a widely employed analytical tool in the biomolecular sciences builds strongly on an effective laser-material interaction that is resulting in a soft co-desorption and ionization of matrix and imbedded biomolecules. To obtain a maximized ion yield for the analyte(s) of interest, in general both wavelength and fluence need to be tuned to match the specific optical absorption profile of the used matrix. However, commonly only lasers with fixed emission wavelengths of either 337 or 355 nm are used for MALDI-MS. Here, we employed a wavelength-tunable dye laser and recorded both the neutral material ejection and the MS ion data in a wide wavelength and fluence range between 280 and 377.5 nm. α-Cyano-4-hydroxycinnamic acid (HCCA), 4-chloro-α-cyanocinnamic acid (ClCCA), α-cyano-2,4-difluorocinnamic acid (DiFCCA), and 2,5-dihydroxybenzoic acid (DHB) were investigated as matrices, and several peptides as analytes. Recording of the material ejection was achieved by adopting a photoacoustic approach. Relative ion yields were derived by division of photoacoustic and ion signals. In this way, distinct wavelength/fluence regions can be identified for which maximum ion yields were obtained. For the tested matrices, optimal results were achieved for wavelengths corresponding to areas of high optical absorption of the respective matrix and at fluences about a factor of 2-3 above the matrix- and wavelength-dependent ion detection threshold fluences. The material ejection as probed by the photoacoustic method is excellently fitted by the quasithermal model, while a sigmoidal function allows for an empirical description of the ion signal-fluence relationship.

Indium Single-Ion Frequency Standard

NASA Technical Reports Server (NTRS)

Nagourney, Warren

2001-01-01

A single laser-cooled indium ion is a promising candidate for an ultimate resolution optical time or frequency standard. It can be shown that single ions from group IIIA of the periodic table (indium, thallium, etc.) can have extremely small systematic errors. In addition to being free from Doppler, transit-time and collisional shifts, these ions are also quite insensitive to perturbations from ambient magnetic and electric fields (mainly due to the use of a J=0-0 transition for spectroscopy). Of all group IIIA ions, indium seems to be the most practical, since it is heavy enough to have a tolerable intercombination cooling transition rate and (unlike thallium) has transitions which are easily accessible with frequency multiplied continuous-wave lasers. A single indium ion standard has a potential inaccuracy of one part in 10(exp 18) for integration times of 10(exp 6) seconds. We have made substantial progress during the grant period in constructing a frequency standard based upon a single indium ion. At the beginning of the grant period, single indium ions were being successfully trapped, but the lasers and optical systems were inadequate to achieve the desired goal. We have considerably improved the stability of the dye laser used to cool the ions and locked it to a molecular resonance line, making it possible to observe stable cooling-line fluorescence from a single indium ion for reasonable periods of time, as required by the demands of precision spectroscopy. We have substantially improved the single-ion fluorescence signal with significant benefits for the detection efficiency of forbidden transitions using the 'shelving' technique. Finally, we have constructed a compact, efficient UV 'clock' laser and observed 'clock' transitions in single indium ions using this laser system. We will elaborate on these accomplishments.

Laser Materials and Laser Spectroscopy - A Satellite Meeting of IQEC '88

NASA Astrophysics Data System (ADS)

Wang, Zhijiang; Zhang, Zhiming

1989-03-01

The Table of Contents for the book is as follows: * Laser Materials * Laser Site Spectroscopy of Transition Metal Ions in Glass * Spectroscopy of Chromium Doped Tunable Laser Materials * Spectroscopic Properties of Nd3+ Ions in LaMgAl11O19 Crystal * Spectral Study and 2.938 μm Laser Emission of Er3+ in the Y3Al5O12 Crystal * Raman-infrared Spectra and Radiationless Relaxation of Laser Crystal NdAl3(BO3)4 * A Study on HB and FLN in BaFCl0.5Br0.5:Sm2+ at 77K * Pair-pumped Upconversion Solid State Lasers * CW Upconversion Laser Action in Neodymium and Erbium doped Solids * Ultra-high Sensitive Upconversion Fluorescence of YbF3 Doped with Trace Tm3+ and Er3+ * The Growth and Properties of NYAB and EYAB Multifunctional Crystal * Study on Fluorescence and Laser Light of Er3+ in Glass * Growth and Properties of Single Crystal Fibers for Laser Materials * A Study on the Quality of Sapphire, Ruby and Ti3+ Doped Sapphire Grown by Temperature Gradient Technique (TGT) and Czochralski Technique (CZ) * The Measurement of Output Property of Ti3+ Al2O3 Laser Crystal * An Xα Study of the Laser Crystal MgF2 : V2+ * Q-switched NAB Laser * Miniature YAG Lasers * Study of High Efficiency {LiF}:{F}^-_2 Color Center Crystals * Study on the Formation Conditions and Optical Properties of (F2+)H Color Center in NaCl:OH- Crystals * Novel Spectroscopic Properties of {LiF}:{F}^+_3 - {F}_2 Mixed Color Centers Laser Crystals * Terraced Substrate Visible GaAlAs Semiconductor Lasers with a Large Optical Cavity * The Temperature Dependence of Gain Spectra, Threshold Current and Auger Recombination in InGaAsP-InP Double Heterojunction Laser diode * Time-resolved Photoluminescence and Energy Transfer of Bound Excitons in GaP:N Crystals * Optical Limiting with Semiconductors * A Critical Review of High-efficiency Crystals for Tunable Lasers * Parametric Scattering in β - BaB2O4 Crystal Induced by Picosecond Pulses * Generation of Picosecond Pulses at 193 nm by Frequency Mixing in β - BaB2O4 * Mixing Frequency Generation of 271.0 - 291.5 nm in β - BaB2O4 * Low Temperature Absorption Steps Near Ultraviolet Intrinsic Edge in Beta Barium Metaborate * The Growth and Properties of BaTiO3 Crystals * High-order Phenomena Accompanied with Self-pumped Phase Conjugation in BaTiO * Growth and Laser Damage Estimation of Potassium Dihydrogen Phosphate Crystals for Laser Fusion * Noncritically Phase-matched KTP for Diode-pumped Lasers (400-700 nm) * Potassium Titanyl Phosphate (KTP): Properties and New Applications * A Kind of New Defect in KTP Crystal and its SHG Enhanced Effect * Nucleation and Growth of the Non-linear Optical Crystal Potassium Pentaborate Tetrahydrate * Quasi-periodic Oscillations in Photoinduced Conical Light Scattering from LiNbO3 : Fe Crystals * Laser Excited Photoreflectance of GaxIn1-xAs/InP Multiple Quantum Wells * Growth, Spectroscopic Properties and Applications of Doped LiNbO3 Crystals * Photorefractive and Photovoltaic Effect in Doped LiNbO3 * Recent Advances in Photorefractive Nonlinear Optics * Study on the Doubling-frequency and Anti-photorefractive Property of Heavily Magnesium-doped Lithium-rich Lithium Niobate Crystals * A New Technique for Increasing Two-wave Mixing Gain in Photorefractive Bi12SiO20 Crystals * Experimental Proof: There Existing Another Mechanism of Photorefractive Index in Crystal Ce-SBN * Effect of Crystal Annealing on Holographic Recording in Bismuth Silicon Oxide * Two Wave Coupling in KNbO3 Photorefractive Crystal * Photorefractive Effects in Nd-Doped Ferroelectric (KxNa1-x)0.4-(SryBa1-y)0.8 Nb2O6 Single Crystal * High Pressure Raman Spectra and the Effect of Pressure to the Ferroelastic Phase Transition in LnP5O15 * Time-delay Four-wave Mixing with Incoherent Light in Absorption Bands Treated as a Multi-level System * Pulsed Laser Induced Dislocation Structure in Lithium Fluoride Single Crystals * Laser Spectroscopy * Nonclassical Radiation from Single-atom Oscillators * Laser Spectroscopic Studies of Molecules in Highly Excited Vibrational State * Investigation of the Stark Effect in Xenon Autoionizing Rydberg Series with the Use of Coherent Tunable XUV Radiation * Laser Spectroscopy of Autoionising 5 dnf J = 4.5 Rydberg Series of Ba I * Resonance Photoionization Spectroscopy of Atoms: Autoionization and Highly Excited States of Kr and U * Stark Spectra of Strontium and Calcium Atoms * Observation of Bidirectional Stimulated Radiation at 330 nm, 364 nm and 718 nm with 660 nm Laser Pumping in Sodium Vapour * Study of Molecular Rydberg States and their Discriminations in Na2 * The Measurement of the High Excited Spectra of Samarium by using Stepwise Laser Excitation Method * Product Analysis in the Reaction of the Two-photon Excited Xe(5p56p) States with Freons * Photoionization Spectra of Ca and Sr Atoms above the Classical Field-ionization Threshold * Effect of Medium Background on the Hydrogen Spectrum * Photoemission and Photoelectron Spectra from Autoionizing Atoms in Strong Laser Field * Natural Radiative Lifetime Measurements of High-lying States of Samarium * Two-step Laser Excitation of nf Rydberg States in Neutral Al and Observation of Stark Effect * Measurements of Excited Spectra of the Refractory Metal Elements using Discharge Synchronized with the Laser Pulse * Multiphoton Ionization of Atomic Lead at 1.06μ * Kinetic Processes in the Electron-beam pumped KrF Laser * Laser-induced Fluorescence of Zn2 Excimer * Calculation of Transition Intensity in Heteronuclear Dimer NaK: Comparison with Experiment * Laser-induced Fluorescence of CCl2 Carbene * Study of Multiphoton Ionization Spectrum of Benzene and Two-photon Absorption Cross Section * Dicke Narrowing of N2O Linewidth Perturbed by N2 at 10 μm Band * Polyatomic Molecular Ions Studied by Laser Photodissociation Spectroscopy * Transverse-optically Pumped Ultraviolet S2 Laser * Multiphoton Ionization of Propanal by High Power Laser * UV MPI Mass Spectroscopy and Dynamics of Photodissociation of SO2 * Multiphoton Ionization-fragmentation Patterns of Ethylamine and Dimethylamine Isomers * Cars Measurements of SF6 Pumped by a CO2 Laser Pulse * Angular Dependence of Phase Conjugation of CO2 Laser on SF6 Gas * Resolution of Stretching-vibrational and Translational Raman Bands of Liquid Water by Means of Polarization Four-photon Spectroscopy * Laser-produced Plasma as an Effective Source for X-Ray Spectroscopy * Rotational Structure of the Low Lying Electronic States of Samarium Monoxide * Effects of Poling and Stretching on Second-harmonic Generation in Amorphous Vinylidene Cyanide/Vinyl Acetate Copolymer * Laser-induced Spectroscopy of Cardiovascular Tissues * Laser-excited Malignancy Autofluorescence for Tumour Malignancy Investigation and its Origin * A Study on Several Hematoporphyrin Derivatives by Time-resolved Spectroscopy * Research on Strong Field Processes with a Subpicosecond 400 GW Ultraviolet Source * Growth, Decay and Quenching of Stimulated Raman Scattering in Transparent Liquid Droplets * Layer Condensed Ammonia Studied by Photoacoustic Spectroscopy * High Efficiency Raman Conversion of XeCl Laser Radiation in Lead Vapor * Combined Effect of Stimulated Scattering and Phase Modulation on Generation of Supercontinum * Resonant Multiwave Mixing in Sodium Vapor * High Pressure Brillouin Scattering in Liquid Toluene * Optical Nonlinearities and Bistability in Gold Colloid * Sum-frequency Generation for Surface Vibrational Spectroscopy * Optical Studies of Molecule/Surface Interactions * Optical Second Harmonic Generation with Coupled Surface Plasmons from a Multi-layer Silver/Quartz Grating * Evidence of Silver Cluster and its Role in Surface Enhanced Raman Scattering (SERS) * Study on Cold-evaporated Silver Surfaces with Second-harmonic-generation * Study of Optical Second-harmonic-generation at Metal Surface with Polarization States * Spectroscopic Studies of J-Aggregates of Pseudoisocyanine in Molecular Monolayers in the Range 300 to 20 K * Study of Polymerization of Langmuir-Blodgett Monolayer by Surface Enhanced Raman Scattering * Dynamics of Laser-induced Etching of Si(III) Surface of Chlorine * Fourier Transform Heterodyne Spectroscopy of Liquid Interfaces * Generation of High Power UV Femtosecond Pulses * Femtosecond Photon Echoes * Transition Radiation of Femtosecond Optical Pulses * Observation of DFWN in a Saturable Absorber inside the CPM Ring Dye Laser Cavity * Study on the Induced Spectral Superbroadening of Ultrafast Laser Pulse in a Nonlinear Medium * Laser Cooling and Trapping of Atoms * Femtosecond Absorption Spectroscopy of Primary Processes in Bacterial Photosynthesis Reaction Centers * Observation of the Motion of Slow Atoms in a Standing Wave Field * The Interrelation between the Optical Properties and the MBE Growth Control of Quantum Well Structures * Ionic Excimers * Optical SHG Study on Polymerization of Langmuir-Blodgett Molecular Layers * Weak Localization of Light * Statistical Fragmentation Patterns of Metastable Ion: Comparison with Experiment * Oxygeneration Reaction of Cerium with XeCl Laser * Measurement of Verdet Coefficient and Magneto-optic Spectroscopy in terms of Beats * Study on Rhodamine 6G/Xylene and Red B Laser Dye Mixture System * Ultranarrow Absorption Resonances of Cold Particles and their Application in Spectroscopy and Optical Frequency Standards * The Dynamics of Ion Clouds in Paul Traps: Implications for Frequency Standard Applications * Frequency Stability Measurement of Zeeman Stabilized He-Ne Laser * Multi-wavelength CW He-Ne Laser and its Frequency Stabilization * Efficient Isotope Separation using Semiconductor Lasers * Multi-beam Circularly Polarized Holography * Ring Laser Opticity Meter * Improved Rademacher Functions and Rademacher Transform * Note

Effects of diode laser welding with dye-enhanced glue on tensile strength of sutures commonly used in urology.

PubMed

Kirsch, A J; Chang, D T; Kayton, M L; Libutti, S K; Connor, J P; Hensle, T W

1996-01-01

Tissue welding using laser-activated protein solders may soon become an alternative to sutured tissue approximation. In most cases, approximating sutures are used both to align tissue edges and provide added tensile strength. Collateral thermal injury, however, may cause disruption of tissue alignment and weaken the tensile strength of sutures. The objective of this study was to evaluate the effect of laser welding on the tensile strength of suture materials used in urologic surgery. Eleven types of sutures were exposed to diode laser energy (power density = 15.9 W/cm2) for 10, 30, and 60 seconds. Each suture was compared with and without the addition of dye-enhanced albumin-based solder. After exposure, each suture material was strained (2"/min) until ultimate breakage on a tensometer and compared to untreated sutures using ANOVA. The strength of undyed sutures were not significantly affected; however, violet and green-dyed sutures were in general weakened by laser exposure in the presence of dye-enhanced glue. Laser activation of the smallest caliber, dyed sutures (7-0) in the presence of glue caused the most significant loss of tensile strength of all sutures tested. These results indicate that the thermal effects of laser welding using our technique decrease the tensile strength of dyed sutures. A thermally resistant suture material (undyed or clear) may prevent disruption of wounds closed by laser welding techniques.

A comparative study of pulsed dye laser versus long pulsed Nd:YAG laser treatment in recalcitrant viral warts.

PubMed

Shin, Yo Sup; Cho, Eun Byul; Park, Eun Joo; Kim, Kwang Ho; Kim, Kwang Joong

2017-08-01

Viral warts are common infectious skin disease induced by human papillomavirus (HPV). But the treatment of recalcitrant warts is still challenging. In this study, we compared the effectiveness of pulsed dye laser (PDL) and long pulsed Nd:YAG (LPNY) laser in the treatment of recalcitrant viral warts. We retrospectively analyzed the medical records of patients with recalcitrant warts treated with laser therapy between January 2013 and February 2016. Seventy-two patients with recalcitrant warts were evaluated. Thirty-nine patients were treated with pulsed dye laser and thirty-three patients were treated with LPNY laser. The following parameters were used: PDL (spot size, 7 mm; pulse duration, 1.5 ms; and fluence, 10-14 J/cm 2 ) and LPNY (spot size, 5 mm; pulse duration, 20 ms; and fluence, 240-300 J/cm 2 ). Complete clearance of two patients (5.1%) in PDL group, and three patients (9.1%) in LPNY group were observed without significant side effects. The patients who achieved at least 50% improvement from baseline were 20 (51.3%) in PDL and 22 (66.7%) in LPNY, respectively. This research is meaningful because we compared the effectiveness of the PDL and LPNY in the recalcitrant warts. Both PDL and LPNY laser could be used as a safe and alternative treatment for recalcitrant warts.

OH Planar Laser Induced Fluorescence (PLIF) Measurements for the Study of High Pressure Flames: An Evaluation of a New Laser and a New Camera System

NASA Technical Reports Server (NTRS)

Tedder, Sarah; Hicks, Yolanda

2012-01-01

Planar laser induced fluorescence (PLIF) is used by the Combustion Branch at the NASA Glenn Research Center (NASA Glenn) to assess the characteristics of the flowfield produced by aircraft fuel injectors. To improve and expand the capabilities of the PLIF system new equipment was installed. The new capabilities of the modified PLIF system are assessed by collecting OH PLIF in a methane/air flame produced by a flat flame burner. Specifically, the modifications characterized are the addition of an injection seeder to a Nd:YAG laser pumping an optical parametric oscillator (OPO) and the use of a new camera with an interline CCD. OH fluorescence results using the injection seeded OPO laser are compared to results using a Nd:YAG pumped dye laser with ultraviolet extender (UVX). Best settings of the new camera for maximum detection of PLIF signal are reported for the controller gain and microchannel plate (MCP) bracket pulsing. Results are also reported from tests of the Dual Image Feature (DIF) mode of the new camera which allows image pairs to be acquired in rapid succession. This allows acquisition of a PLIF image and a background signal almost simultaneously. Saturation effects in the new camera were also investigated and are reported.

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke.

PubMed

Talley Watts, Lora; Zheng, Wei; Garling, R Justin; Frohlich, Victoria C; Lechleiter, James Donald

2015-06-23

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to image cellular events in an intact animal. Additionally, the ability to induce physiological disease states such as stroke in vivo increases its utility. The technique described herein allows for physiological assessment of cellular responses within the CNS following a stroke and can be adapted for other pathological conditions being studied. The technique presented uses laser excitation of the photosensitive dye Rose Bengal in vivo to induce a focal ischemic event in a single blood vessel. The video protocol demonstrates the preparation of a thin-skulled cranial window over the somatosensory cortex in a mouse for the induction of a Rose Bengal photothrombotic event keeping injury to the underlying dura matter and brain at a minimum. Surgical preparation is initially performed under a dissecting microscope with a custom-made surgical/imaging platform, which is then transferred to a confocal microscope equipped with an inverted objective adaptor. Representative images acquired utilizing this protocol are presented as well as time-lapse sequences of stroke induction. This technique is powerful in that the same area can be imaged repeatedly on subsequent days facilitating longitudinal in vivo studies of pathological processes following stroke.

Optical Sensor based Chemical Modification as a Porous Cellulose Acetate Film and Its Application for Ethanol Sensor

NASA Astrophysics Data System (ADS)

Mulijani, S.; Iswantini, D.; Wicaksono, R.; Notriawan, D.

2018-03-01

A new approach to design and construction of an optical ethanol sensor has been developed by immobilizing a direct dye at a porous cellulosic polymer fllm. This sensor was fabricated by binding Nile Red to a cellulose acetate membrane that had previously been subjected to an exhaustive base hydrolysis. The prepared optical ethanol sensor was enhanced by adding pluronic as a porogen in the membrane. The addition of pluronic surfactant into cellulose acetate membrane increased the hydrophilic and porous properties of membrane. Advantageous features of the design include simple and easy of fabrication. Variable affecting sensor performance of dye concentration have been fully evaluated and optimized. The rapid response results from the porous structure of the polymeric support, which minimizes barriers to mass transport. Signal of optical sensor based on reaction of dye nile red over the membrane with ethanol and will produce the purple colored product. Result was obtained that maximum intensity of dye nile red reacted with alcohol is at 630-640 nm. Linear regression equation (r2), limit of detection, and limit of quantitation of membrane with 2% dye was 0.9625, 0.29%, and 0.97%. Performance of optical sensor was also evaluated through methanol, ethanol and propanol. This study was purposed to measure the polarity and selectivity of optic sensor toward the alcohol derivatives. Fluorescence intensity of optic sensor membrane for methanol 5%, ethanol 5% and propanol 5% was 15113.56, 16573.75 and 18495.97 respectively.

Tuning Solvatochromism of Azo Dyes with Intramolecular Hydrogen Bonding in Solution and on Titanium Dioxide Nanoparticles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Lei; Cole, Jacqueline M.; Liu, Xiaogang

2013-11-25

“Smart tuning” of optical properties in three azo dyes containing intramolecular hydrogen bonding is realized by the judicious control of solvents, when the dyes are in solution or adsorbed onto titanium dioxide nanoparticles. In solution, certain solvents destabilizing intramolecular hydrogen bonding induce a distinctive ≈70 nm “blue-shifted” absorption peak, compared with other solvents. In parallel, the optical properties of azo dye/TiO2 nanocomposites can be tuned using solvents with different hydrogen-bond accepting/donating abilities, giving insights into smart materials and dye-sensitized solar cell device design. It is proposed that intramolecular hydrogen bonding alone plays the leading role in such phenomena, which ismore » fundamentally different to other mechanisms, such as tautomerism and cis–trans isomerization, that explain the optical control of azo dyes. Hybrid density functional theory (DFT) is employed in order to trace the origin of this optical control, and these calculations support the mechanism involving intramolecular hydrogen bonding. Two complementary studies are also reported: 1H NMR spectroscopy is conducted in order to further understand the solvent effects on intramolecular hydrogen bonding; crystal structure analysis from associated research indicates the importance of intramolecular hydrogen bonding on intramolecular charge transfer.« less

Passive mode lockers for lasers generating at a wavelength of 1.06 micron

NASA Astrophysics Data System (ADS)

Mikhailov, V. P.; Demchuk, M. I.; Lugovskii, A. P.; Sosnovskii, G. M.; Iumashev, K. V.

1983-04-01

New polymethine dyes that can be used for the passive mode locking of lasers generating at 1.06 micron are investigated using YAG:Nd as the active element. The effect of introducing various substituents into the heterocyclic nuclei of the end groups of polymethine dyes is discussed. It is shown that substituents generally increase the energy of the ultrashort pulse while also increasing its length.

Topical timolol maleate 0.5% for infantile hemangioma; it's effectiveness and/or adjunctive pulsed dye laser - single center experience of 102 cases in Korea.

PubMed

Park, Kyung Hea; Jang, Yong Hyun; Chung, Ho Yun; Lee, Weon Ju; Kim, Do Won; Lee, Seok-Jong

2015-01-01

Although oral beta-blocker, propranolol, was shown excellent outcome for infantile hemangioma (IH) up to date, concern of side effects and reluctance of treatment-related cumbersome evaluations are major obstacles to employ. Instead, topical beta-blockers were recently introduced as an effective alternative, but few studies are reported. So we performed a retrospective study of IH treated with topical beta-blockers, timolol maleate 0.5%, and adjunctive role of pulsed dye laser from 2011 to 2014. Among 102 IH enrolled, 61 patients (59.8%) treated with only timolol maleate and 41 (40.2%) patients treated with combination of pulsed dye laser. A clinical review of medical records and evaluation at 4-8 weeks intervals using the physicians' Global Assessment Scores (GAS) and patients' parents' GAS at the latest visit. Physicians' GAS was used to grade the lesions compared with the baseline photo by two physicians' evaluation. And parents' GAS was assessed by direct or telephone interview. In the only timolol treatment group, mean change was within 47.0% improvement from baseline by physicians. In addition, adjunctive treatment of pulsed dye laser group showed 66.5% improvement. No side effects were found on both groups, and mean change was 54.5% improvement by overall parent assessments.

Pulsed Dye Laser Therapy in the Treatment of Warts: A Review of the Literature.

PubMed

Veitch, David; Kravvas, Georgios; Al-Niaimi, Firas

2017-04-01

Warts or verrucae vulgaris are common cutaneous infections with currently no definitive curative treatments available. To determine the efficacy of pulsed dye laser (PDL) in the treatment of warts. A literature search was performed using the PubMed and MEDLINE databases. A search using {(Wart[s], verruca or condylomata)} AND [(Pulsed dye laser)] was used. Forty-four articles were identified as relevant to this review. Simple warts were very responsive to PDL, being treated successfully in over 95% of patients. Facial and anogenital warts also demonstrated excellent outcomes. Recalcitrant warts, displayed significant variability in their response, ranging between 50% and 100% across all articles. The response rates seen in peripheral warts (involving the hands and feet) were also very variable, ranging between 48% and 95%. Recurrence rates at 4 months of follow-up were documented as 0% to 15%. Complications have been described as very few and rare, the main ones being topical discomfort and erythema. Pulsed dye laser is a safe and effective modality in the treatment of warts that can be applied to most body parts. Cost and availability remain a limitation to the use of PDL; however, this modality can be used when other more traditional and accessible treatments have failed.

Studies on the optogalvanic effect and isotope-selective excitation of ytterbium in a hollow cathode discharge lamp using a pulsed dye laser.

PubMed

Kumar, Pankaj; Kumar, Jitendra; Prakash, Om; Saini, Vinod K; Dixit, Sudhir K; Nakhe, Shankar V

2013-09-01

This paper presents studies on the pulsed optogalvanic effect and isotope-selective excitation of Yb 555.648 nm (0 cm(-1) → 17 992.007 cm(-1)) and 581.067 nm (17 992.007 cm(-1) → 35 196.98 cm(-1)) transitions, in a Yb/Ne hollow cathode lamp. The Yb atoms were excited by narrow linewidth (500-1000 MHz) Rh110 and Rh6G dye based pulsed lasers. Optogalvanic signal inversion for ground state transition at 555.648 nm was observed beyond a hollow cathode discharge current of 8.5 mA, in contrast to normal optogalvanic signal at 581.067 nm up to maximum current of 14 mA. The isotope-selective excitation studies of Yb were carried out by recording Doppler limited optogalvanic signals as a function of dye laser wavelength. For the 581.067 nm transition, three even isotopes, (172)Yb, (174)Yb, and (176)Yb, and one odd isotope, (171)Yb, were clearly resolved. These data were compared with selective isotope excitation by 10 MHz linewidth continuous-wave dye laser. For 555.648 nm transition, isotopes were not clearly resolved, although isotope peaks of low modulation were observed.

Studies on the optical and photoelectric properties of anthocyanin and chlorophyll as natural co-sensitizers in dye sensitized solar cell

NASA Astrophysics Data System (ADS)

Nan, Hui; Shen, He-Ping; Wang, Gang; Xie, Shou-Dong; Yang, Gui-Jun; Lin, Hong

2017-11-01

Anthocyanin and Chlorophyll extracted from Troll flower and Cypress leaf respectively are used as natural sensitizers in dye sensitized solar cells (DSCs), with their optical and electrochemical properties investigated. UV-Vis absorption measurement showed that the mixture of two dyes enabled an enhanced and wider absorption in the wavelength range of 300 nm-700 nm compared to each single dye. FTIR results proved that anthocyanin is chemically adsorbed onto the TiO2 film, while it is physical adsorption for chlorophyll. The energy level offsets on the TiO2/dye/electrolyte interface for each dye and the dye mixture with different ratios were calculated from the electrochemical analysis, which affect the electron injection and dye regeneration efficiencies. The optimized ratio of the two dyes in the mixture was found to be ∼2:5, inducing both sufficient charge transfer driving force and minimal energy loss. By incorporating this mixture into the solar cell as co-adsorbing sensitizer, the photovoltaic performance was prominently improved compared with the single dye sensitization system.

Amplification of spontaneous emission on sodium D-lines using nonresonance broadband optical pumping

NASA Astrophysics Data System (ADS)

Petukhov, T. D.; Evtushenko, G. S.; Tel'minov, E. N.

2018-04-01

This work describes an experimental study of obtaining the amplified spontaneous emission (ASE) on sodium D-lines using nonresonance broadband optical pumping. ASE is observed at transitions D2 and D1 line: 589 nm (32 P3/2 - 32 S1/2) and 589.6 nm (32 P1/2 - 32 S1/2). The active medium was pumped by the dye laser with FWHM of 5 nm, maximum radiation in the range 584.5-586.5 nm, and pulse energy above 2 mJ. The working temperature of the active medium was 260 °C, initial pressure of buffer gas-helium was 300 torr (operating pressure - 500 torr). A change in the absorption spectra at D lines at different temperatures of the active medium and buffer gas pressures was observed

Density of photon states in dye-doped chiral nematic liquid crystal cells in the presence of losses and gain.

PubMed

Mavrogordatos, Th K; Morris, S M; Castles, F; Hands, P J W; Ford, A D; Coles, H J; Wilkinson, T D

2012-07-01

We calculate the density of photon states (DOS) of the normal modes in dye-doped chiral nematic liquid crystal (LC) cells in the presence of various loss mechanisms. Losses and gain are incorporated into the transmission characteristics through the introduction of a small imaginary part in the dielectric constant perpendicular and along the director, for which we assume no frequency dispersion. Theoretical results are presented on the DOS in the region of the photonic band gap for a range of values of the loss coefficient and different values of the optical anisotropy. The obtained values of the DOS at the photonic band gap edges predict a reversal of the dominant modes in the structure. Our results are found to be in good agreement with the experimentally obtained excitation thresholds in chiral nematic LC lasers. The behavior of the DOS is also discussed for amplifying LC cells providing additional insight to the lasing mechanism of these structures.

Sensitive optical bio-sensing of p-type WSe2 hybridized with fluorescent dye attached DNA by doping and de-doping effects

NASA Astrophysics Data System (ADS)

Han, Kyu Hyun; Kim, Jun Young; Jo, Seong Gi; Seo, Changwon; Kim, Jeongyong; Joo, Jinsoo

2017-10-01

Layered transition metal dichalcogenides, such as MoS2, WSe2 and WS2, are exciting two-dimensional (2D) materials because they possess tunable optical and electrical properties that depend on the number of layers. In this study, the nanoscale photoluminescence (PL) characteristics of the p-type WSe2 monolayer, and WSe2 layers hybridized with the fluorescent dye Cy3 attached to probe-DNA (Cy3/p-DNA), have been investigated as a function of the concentration of Cy3/DNA by using high-resolution laser confocal microscopy. With increasing concentration of Cy3/p-DNA, the measured PL intensity decreases and its peak is red-shifted, suggesting that the WSe2 layer has been p-type doped with Cy3/p-DNA. Then, the PL intensity of the WSe2/Cy3/p-DNA hybrid system increases and the peak is blue-shifted through hybridization with relatively small amounts of target-DNA (t-DNA) (50-100 nM). This effect originates from charge and energy transfer from the Cy3/DNA to the WSe2. For t-DNA detection, our systems using p-type WSe2 have the merit in terms of the increase of PL intensity. The p-type WSe2 monolayers can be a promising nanoscale 2D material for sensitive optical bio-sensing based on the doping and de-doping responses to biomaterials.

Nonlinear-optical studies of organic liquids and polymer optical fibers

NASA Astrophysics Data System (ADS)

Vigil, Steven Richard

We present the results of non-resonant optical Kerr effect measurements of the neat organic liquids nitrobenzene, carbon disulfide, carbon tetrachloride, and methyl-methacrylate. We also present the results of one- photon resonant optical Kerr effect measurements of solutions of the organic dyes indole squarylium and silicon-phthalocyanine:methylmethacrylate (separately) in carbon tetrachloride. Fits of the molecular third-order susceptibility theory to the one-photon resonant data indicate the presence of high-lying two-photon states for each of the dye molecules studied. We also present results concerning light coupling in dye- doped dual-core polymer optical fibers. Measurements of the coupling length at low intensity are in agreement with linear coupling length calculations. Intensity- dependent coupling is observed as the intensity launched into the fiber is increased.

Development of a measurement system for nitrate radical and dinitrogen pentoxide using a thermal conversion/laser-induced fluorescence technique

NASA Astrophysics Data System (ADS)

Matsumoto, Jun; Kosugi, Naohiro; Imai, Hidekazu; Kajii, Yoshizumi

2005-06-01

An instrument for measuring atmospheric nitrate radical (NO3) and dinitrogen pentoxide (N2O5) has been developed by a thermal conversion/laser-induced fluorescence (TC/LIF) technique. N2O5 is thermally decomposed and converted to NO3, which is measured by laser-induced fluorescence. In situ, fast-response, sensitive measurement of NO3/N2O5 is expected by use of LIF. In detecting NO3, dual-wavelength excitation at 622.96 and 618.81nm was adopted to remove potential interference and to guarantee high selectivity. A high-power dye laser system was used as the source of excitation light. To measure ambient air directly, the NO3 detection cell was placed on the rooftop. The laser beam was guided by an optical fiber into the excitation cell. Transmittance of the laser beam was 80% for a 10m long fiber. To calibrate the instrument, the series of thermal decomposition of N2O5 and the gas phase titration of NO3 by NO were conducted. NO3 reduction by adding NO was also applied to determine accurately the zero points of the detector. After optimization of conditions such as gate timing in photon counting and the settings of the N2O5 converter, the present detection limits of NO3 and N2O5 were determined to be 4 and 6pptv, respectively, for the integration time of 10min (signal-to-noise ratio=1). It was confirmed that the interference of NO2 on N2O5 detection is negligible, but can be significant for NO3 measurement when NO2 concentration is extremely high (>100ppbv ). Measurement of N2O5 in ambient air was made in the urban area of Tokyo, Japan. Observed data demonstrated the capacity of the TC/LIF instrument with a powerful dye laser and a single-path excitation cell for ambient measurements. In this article, we focus on the instrumentation and characterization.

Smartphone Based Platform for Colorimetric Sensing of Dyes

NASA Astrophysics Data System (ADS)

Dutta, Sibasish; Nath, Pabitra

We demonstrate the working of a smartphone based optical sensor for measuring absorption band of coloured dyes. By integration of simple laboratory optical components with the camera unit of the smartphone we have converted it into a visible spectrometer with a pixel resolution of 0.345 nm/pixel. Light from a broadband optical source is allowed to transmit through a specific dye solution. The transmitted light signal is captured by the camera of the smartphone. The present sensor is inexpensive, portable and light weight making it an ideal handy sensor suitable for different on-field sensing.