Rural Resources.

ERIC Educational Resources Information Center

Novak, Kathy

Designed as a resource for rural adult basic education (ABE) program planners, this guidebook describes model linkage strategies between ABE and job placement as well as ABE and job training services that are targeted to rural Americans. The following topics are addressed in the guide: key linkage strategies (community advisory councils,…

Adult Basic Education: Research, Demonstration, Staff Development and Dissemination. Proceedings of the 1978 Virgina ABE Dissemination Conference (Ingleside Resort Hotel, Staunton, Virginia, July 31-August 2, 1978).

ERIC Educational Resources Information Center

Virginia State Dept. of Education, Richmond. Adult Education Service.

This conference proceedings report contains abstracts of seven 1977-78 Virginia Adult Basic Education (ABE) projects presented at a dissemination conference for ABE administrators and teachers. The abstracts vary in length (two to seven pages) and format and focus on program objectives, procedures or strategies, expected results, findings,…

The Functional Illiterate: Is Correctional Education Doing Its Job?

ERIC Educational Resources Information Center

Loeffler, Cynthia A.; Martin, Thomas C.

A study researched the existence of established Adult Basic Education (ABE) curricula for incarcerated adult inmate/students in state correctional education programs, specifically the functionally illiterate. All 50 State Departments of Corrections were surveyed by questionnaire; 44 responded. ABE was a basis for curricula according to 37.6% of…

Urban Resources.

ERIC Educational Resources Information Center

Novak, Kathy

Designed as a resource for urban adult basic education (ABE) program planners, this guidebook describes model linkage strategies between ABE and job placement as well as ABE and job training services that are targeted to urban Americans. The following topics are covered in the guide: linkage strategies (the meaning of the term linkages, community…

The ABE/AMH Manual. An Instructional Guide for ABE Programs Serving Mentally Handicapped Adults.

ERIC Educational Resources Information Center

Lehman, Scott C.; Edgar, S. Keith

This handbook provides adult basic education teachers with instructional materials for working with adult mentally handicapped students. Section 1 examines planning programs for adult mentally retarded students (getting started, specific considerations, various kinds of program sites) and implementing instruction (staff selection and training).…

Additional and Realistic Dimensions for Adult Basic Education - ABE for Real?

ERIC Educational Resources Information Center

Patten, W. George

Adult basic education (ABE) programs are not adequate to prepare the unemployed and undereducated to gain entry into the labor market and conceivably higher education. Some indications of the scope of the problem can be seen in a recent Division of Adult Education, U.S. Office of Education, report which indicated that more than fifty-one million…

Documenting Outcomes for Learners and Their Communities: A Report on a NCSALL Action Research Project. NCSALL Reports.

ERIC Educational Resources Information Center

Bingman, Mary Beth

Action research focused on developing approaches local programs can use to document outcomes of student participation in adult basic education (ABE) programs. Teams of teachers and administrators from three ABE programs examined current documentation practices, were introduced to approaches to documentaion, and developed documentation processes…

Barriers to Persistence in Adult Basic Education: The Experiences of African American Learners

ERIC Educational Resources Information Center

Thomas, Simone

2011-01-01

One of the most pervasive issues facing educators and administrators in Adult Basic Education (ABE) is student persistence. The purposes of this qualitative study were 1) to identify the experiences that African American adult learners associated with their decisions to leave ABE programs; 2) to ascertain the impact of participants'…

Counseling the Adult Basic Education Student.

ERIC Educational Resources Information Center

Champagne, Delight E.

This guide is designed for use by counselors and teachers working with adult basic education (ABE) students. Discussed first is the importance of viewing adult learners as clients. The unique characteristics of the ABE student are examined. Presented next is an adult counseling model that includes the following program participation phases:…

The BEST Blueprint. Quality ABE in the Workplace.

ERIC Educational Resources Information Center

Westberry, Susan

The Basic Educational Skills Training (BEST) workplace literacy demonstration model was designed to provide adult basic education (ABE) services simultaneously for multiple employers in Maury County, Tennessee. The BEST model focused on job-related instruction. The goal of the program was to achieve increased safety, productivity, and employee…

Computer Assisted Teaching Comparisons with Handicapped. Final Report.

ERIC Educational Resources Information Center

Main, JoDell K.

A project was conducted to see if computer-assisted instruction could be used successfully with the low-level, non-reading adult. The experimental classroom group consisted of mentally handicapped and other educationally handicapped adults in adult basic education (ABE) programs. (Long-range implementation is aimed at ABE students who have a…

Learning Difficulties and the Power of Labelling in ABE. Mendip Papers MP071.

ERIC Educational Resources Information Center

Bergin, Sue; Johnson, Andy

A study examined recent developments in adult basic education (ABE) in Great Britain in relation to students with learning difficulties and issues about the ways in which programs seemed to be moving. Information was collected from ABE staff and students in case study sites in northwest England from the following sources: semistructured interviews…

Project Reach: Annual Report--Year 1. June 1, 1969-June 14, 1970.

ERIC Educational Resources Information Center

McClelland, Samuel D.

Project Reach, a Federally funded two-year program at the University of Notre Dame, studied three methods of increasing the scope and effectiveness of a local adult basic education (ABE) program in St. Joseph County, Indiana. The methods were: (1) a paraprofessional training program for six former ABE students in still photography, color film…

Pilot Project in Computer Assisted Instruction for Adult Basic Education Students. Adult Learning Centers, the Adult Program, 1982-83.

ERIC Educational Resources Information Center

Buckley, Elizabeth; Johnston, Peter

In February 1977, computer assisted instruction (CAI) was introducted to the Great Neck Adult Learning Centers (GNALC) to promote greater cognitive and affective growth of educationally disadvantaged adults. The project expanded to include not only adult basic education (ABE) students studying in the learning laboratory, but also ABE students…

Toward a New Pluralism in ABE/ESOL Classrooms: Teaching to Multiple "Cultures of Mind." Research Monograph. NCSALL Reports.

ERIC Educational Resources Information Center

Kegan, Robert; Broderick, Maria; Drago-Severson, Eleanor; Helsing, Deborah; Popp, Nancy; Portnow, Kathryn

This document contains information about and from a study of the experiences of 41 adults enrolled in adult basic education/English for speakers of other languages (ABE/ESOL) programs that was conducted to determine what their learning meant to them and to identify strategies for developing a new pluralism in ABE/ESOL classrooms and teaching to…

A Participative Process for the Design and Production of Adult Basic Education Training Resources.

ERIC Educational Resources Information Center

Villa, Jane Kathryn; Chalmers, Michael

1981-01-01

Reviews the three-stage participative process used to create and test resources for Adult Basic Education (ABE) staff development in North Carolina. Introduces the 1980 State plan for ABE and its objectives. Describes workshops for program directors and instructors which resulted in staff development handbooks. Explains the evaluation process.…

The Use of the Adjective Check List to Describe the Adult Basic Education Student.

ERIC Educational Resources Information Center

Wilson, Russell C.

The study's purpose was to use the Adjective Check List (ACL), a self-administered personality assessment instrument, to determine whether there were response differences between a sample of Adult Basic Education (ABE) students and the general test norms. The ACL was administered to 142 students in ABE high school completion programs in Des…

Adult Basic Education: British Columbia's Public Post-Secondary Institutions. An Articulation Handbook. 2008-2009 Edition

ERIC Educational Resources Information Center

Ministry of Advanced Education and Labour Market Development, 2008

2008-01-01

The purpose of Adult Basic Education (ABE) articulation is to ensure learners have access to quality courses, receive appropriate credits and are able to transfer easily among publicly-funded colleges, university colleges, universities and institutes in British Columbia. Articulation also involves liaison with the school system's ABE programs to…

A Statewide Profile of Adult Basic Education.

ERIC Educational Resources Information Center

Essex, Martin W.; And Others

A survey of 72 adult basic education (ABE) programs in Ohio was conducted during the 1968-69 school year. Sixty-nine directors handled 447 ABE classes; however, about 40% were handled by three of them. Public school classrooms were the most frequently used facilities (68%). Night classes accounted for 85%. Most of the directors' time was devoted…

Defining Success in Adult Basic Education Settings: Multiple Stakeholders, Multiple Perspectives

PubMed Central

Tighe, Elizabeth L.; Barnes, Adrienne E.; Connor, Carol M.; Steadman, Sharilyn C.

2015-01-01

This study employed quantitative and qualitative research approaches to investigate what constitutes “success” in Adult Basic Education (ABE) programs from the perspectives of multiple educational stakeholders: the state funding agency, the teachers, and the students. Success was defined in multiple ways. In the quantitative section of the study, we computed classroom value-added scores (used as a metric of the state’s definition of success) to identify more and less effective ABE classrooms in two Florida counties. In the qualitative section of the study, we observed and conducted interviews with teachers and students in the selected classrooms to investigate how these stakeholders defined success in ABE. Iterative consideration of the qualitative data revealed three principal markers of success: (a) instructional strategies and teacher-student interactions; (b) views on standardized testing; and (c) student motivational factors. In general, classrooms with higher value-added scores were characterized by multiple instructional approaches, positive and collaborative teacher-student interactions, and students engaging in goal setting and citing motivational factors such as family and personal fulfillment. The implications for ABE programs are discussed. PMID:26279590

Practices and Problems of Adult Basic Education in Rural Areas.

ERIC Educational Resources Information Center

Richardson, E. Gordon

The percentages of adults needing adult basic education (ABE) programs in rural areas may not differ from those found in metropolitan areas, but the delivery of the system may be different. For example, the rural ABE teaching staff probably will be recruited from the ranks of the regular elementary or high school teachers to teach at night also,…

25 CFR 46.2 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-04-01

... formal secondary or post-secondary educational program. Adult Basic Education (ABE) means instruction... AFFAIRS, DEPARTMENT OF THE INTERIOR EDUCATION ADULT EDUCATION PROGRAM General Provisions § 46.2... Education means services or instruction below the college level for adults who: (1) Lack sufficient mastery...

25 CFR 46.2 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... formal secondary or post-secondary educational program. Adult Basic Education (ABE) means instruction... AFFAIRS, DEPARTMENT OF THE INTERIOR EDUCATION ADULT EDUCATION PROGRAM General Provisions § 46.2... Education means services or instruction below the college level for adults who: (1) Lack sufficient mastery...

25 CFR 46.2 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-04-01

... formal secondary or post-secondary educational program. Adult Basic Education (ABE) means instruction... AFFAIRS, DEPARTMENT OF THE INTERIOR EDUCATION ADULT EDUCATION PROGRAM General Provisions § 46.2... Education means services or instruction below the college level for adults who: (1) Lack sufficient mastery...

25 CFR 46.2 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-04-01

... formal secondary or post-secondary educational program. Adult Basic Education (ABE) means instruction... AFFAIRS, DEPARTMENT OF THE INTERIOR EDUCATION ADULT EDUCATION PROGRAM General Provisions § 46.2... Education means services or instruction below the college level for adults who: (1) Lack sufficient mastery...

25 CFR 46.2 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-04-01

... formal secondary or post-secondary educational program. Adult Basic Education (ABE) means instruction... AFFAIRS, DEPARTMENT OF THE INTERIOR EDUCATION ADULT EDUCATION PROGRAM General Provisions § 46.2... Education means services or instruction below the college level for adults who: (1) Lack sufficient mastery...

Opportunities Unlimited: Minnesota Indians Adult Basic Education; Narrative and Statistical Evaluation Third Year 1971-72, with a Review of the First and Second Years.

ERIC Educational Resources Information Center

Vizenor, Gerald

Opportunities Unlimited is a State-wide program to provide adult basic education (ABE) and training for Indians on Minnesota reservations and in Indian communities. An administrative center in Bemidji serves communities on the Red Lake, White Earth, and Leech Lake Reservations, and a Duluth center provides ABE and training for communities on the…

Project Evolve User-Adopter Manual.

ERIC Educational Resources Information Center

Joiner, Lee M.

An adult basic education (ABE) program for mentally retarded young adults between the ages of 14 and 26 years, Project Evolve can provide education agencies for educationally handicapped children with detailed information concerning an innovative program. The manual format was developed through interviews with professional educators concerning the…

Unmet Needs of Low Academic Level Adult (0-4th Grade Level) Students: A Follow-Up Study. A Special Demonstration/Teacher Training Project. Final Report.

ERIC Educational Resources Information Center

Portage Township Schools, IN.

An Indiana 310 Project was conducted to determine the needs of very low level adult basic education students (0-4th grade). Specifically, the study sought to answer the following four questions: (1) What brings low academic level students into ABE programs? (2) What aspects of the ABE programs do low academic level students dislike? (3) Why do low…

California Update: Student Progress in State and Federally Funded Adult Education Programs during the 1997-98 Instructional Year.

ERIC Educational Resources Information Center

Comprehensive Adult Student Assessment System, San Diego, CA.

This document outlines student progress within each of California's Adult Education programs for the 1997-1998 academic year. During this time period, California's Adult Education programs served 1,435,341 learners. Among those enrolled, 161,364 students were served by Adult Basic Education (ABE) programs, and an additional 1,220,594 students were…

Adult Basic Education 1985-1986 End-of-Year Report.

ERIC Educational Resources Information Center

Mei, Dolores M.; And Others

The Adult Basic Education/High School Equivalency (ABE/HSE) Services Program provides basic educational services for out-of-school youth and adults in New York City. The program offers classes in basic literacy (BL), basic education (BE), high school equivalency (HSE), and English as a second language (ESL). The program's budget is $11 million.…

Working toward Literacy in Correctional Education ESL

ERIC Educational Resources Information Center

Gardner, Susanne

2014-01-01

Correctional Education English as a Second Language (ESL) literacy programs vary from state to state, region to region. Some states enroll their correctional ESL students in adult basic education (ABE) classes; other states have separate classes and programs. At the Maryland Correctional Institution in Jessup, the ESL class is a self-contained…

Dispositional Factors Affecting Motivation during Learning in Adult Basic and Secondary Education Programs

ERIC Educational Resources Information Center

Mellard, Daryl F.; Krieshok, Thomas; Fall, Emily; Woods, Kari

2013-01-01

Research indicates that about a quarter of adult students separate from formal adult basic and secondary education (ABE/ASE) programs before completing one educational level. This retrospective study explores individual dispositional factors that affect motivation during learning, particularly students' goals, goal-directed thinking and action…

Empowering Adult Learners. NIF Literacy Program Helps ABE Accomplish Human Development Mission.

ERIC Educational Resources Information Center

Hurley, Mary E.

1991-01-01

The National Issues Forum's Literacy Program uses study circles and group discussion to promote empowerment and enhance adult literacy through civic education. The program has helped the Westonka (Minnesota) Adult Basic Education project accomplish its mission and has expanded the staff's view of adult learning. (SK)

Adult Education Association of the U.S.A.; Adult Basic Education Study 1965-66.

ERIC Educational Resources Information Center

Firoza, Ahmed, Ed.

The adult basic education (ABE) programs currently conducted by non-governmental organizations in the United States, are reviewed in this document. Attention is focused on the significance of voluntary efforts in adult basic education programs; and strengths and weaknesses, gaps between needs and resources, and limiting factors are identified.…

Adult Basic Education and the Welfare Roles: An Economic and Social Alternative.

ERIC Educational Resources Information Center

Pennsylvania Association for Adult Continuing Education, Harrisburg, PA.

In Pennsylvania where 30 percent of the adult population is functionally illiterate and another 24 percent has only marginal competence, no state funds are appropriated for adult basic education and general educational development (ABE/GED) programs. All programs are supported by federal aid. Information shows that economic revitalization and a…

An Introduction to the Process-Conference Approach to the Teaching of Writing Skills in ABE Programs.

ERIC Educational Resources Information Center

Rutledge, George E.

Writing is an important tool in teaching skills to adult basic education (ABE) students. To be a successful teacher of writing, teachers must be successful writers. They must be aware of the writing process and willing to use it daily in their own lives in order to convey its importance and its usefulness to their students. One method of teaching…

310 Individualized Teacher Practicum. Final Report, 1979-80.

ERIC Educational Resources Information Center

Barabe, Rosemeri, Comp.; And Others

Objectives and program descriptions are presented for the Scottsdale Adult Learning Center (Arizona) which in 1979-80 conducted a number of practicums for adult educators on individualized techniques, Adult Basic Education (ABE), High School Equivalency (GED), and English as a Second Language (ESL). First described is a paid internship program for…

Does Prison-Based Adult Basic Education Improve Postrelease Outcomes for Male Prisoners in Florida?

PubMed Central

Cho, Rosa Minhyo; Tyler, John H.

2014-01-01

The authors use administrative data from Florida to determine the extent to which prison-based adult basic education (ABE) improves inmate’s postrelease labor market outcomes, such as earnings and employment. Using two nonexperimental comparison groups, the authors find evidence that ABE participation is associated with higher postrelease earnings and employment rates, especially for minorities. The authors find that the relationship is the largest for ABE participants who had uninterrupted ABE instruction and for those who received other education services. However, the results do not find any positive effects of ABE participation on reducing recidivism. PMID:25580020

A Countywide Adult Basic Education Program. Final Report, 1986-1987.

ERIC Educational Resources Information Center

Vanis, Mary I.; Mills, Karen L.

In July 1979, the countywide Adult Basic Education (ABE) Program at Rio Salado Community College (RSCC) was established to provide adult basic education services to Maricopa County (Arizona) residents. Between 1979 and 1987, enrollments rose from 729 students to more than 5,700 students. A major contributing factor to the on-going growth lies in…

Virginia State Adult Basic Education Administrative Guide for Local Programs and Projects under the Adult Education Act, P.L. 91-230 and Amendments.

ERIC Educational Resources Information Center

Virginia State Dept. of Education, Richmond. Adult Education Service.

This administrative guide was developed to provide local school divisions and other agencies operating federally funded Adult Basic Education (ABE) programs in Virginia with the purpose, requirements, and procedures for conducting these programs. The guide is divided into eleven sections. The introduction covers the purpose and scope of ABE…

Organizational Development and Its Implications for Adult Basic Education Programs

ERIC Educational Resources Information Center

Hohn, Marcia Drew

2001-01-01

In this chapter, Marcia Drew Hohn provides an overview of organizational development theory for adult educators interested in applying the lessons of such theory to the strengthening of ABE programs and systems. She begins the chapter with a brief history of the development of organizational theory, noting the progression from a mechanistic…

Workforce Education. Hotel and Motel Workers. A Section 353 Demonstration Project.

ERIC Educational Resources Information Center

Polk County Public Schools, Bartow, FL.

This guide provides an overall view of a program designed to educate adult basic education (ABE) and English-as-a-Second-Language (ESL) students in job-related, language-oriented skills vital to their positions in the hotel/motel industry. The program was designed for the employees of Grenelefe Resort and Conference Center in Haines City, Florida,…

Building the Bridge: Inservice Training for Teachers of Educationally Disadvantaged. Final Report: July 1, 1980-August 31, 1981.

ERIC Educational Resources Information Center

Bitterlin, Gretchen

This three-part report describes the Bridging Program at San Diego Mesa College (SDMC), a basic skills program to prepare students functioning at the 7th grade level or below and to thus provide a bridge between developmental education and adult basic education (ABE). Part I begins with a review of the program's background and a list of its…

New Skills for a New Economy: Adult Education's Key Role in Sustaining Economic Growth and Expanding Opportunity.

ERIC Educational Resources Information Center

Comings, John; Sum, Andrew; Uvin, Johan

The role of adult education in sustaining economic growth and expanding opportunity in Massachusetts was explored. The analysis focused on the new basic skills needed for a new economy, groups lacking the new basic skills, the demand for adult basic education (ABE), funding for ABE, building basic skills through adult education, ABE's costs and…

Policy to Performance: State ABE Transition Systems Report. Transitioning Adults to Opportunity

ERIC Educational Resources Information Center

Alamprese, Judith A.

2012-01-01

The U.S. Department of Education's Policy to Performance project was funded in 2009 to build the capacity of state adult basic education (ABE) staff to develop and implement policies and practices that would support an ABE transition system. Policy to Performance states were selected though a competitive process. State adult education directors…

Effect of Instructional Design on Academic Success of Adult Basic Education Learners: Individualized versus Group Design

ERIC Educational Resources Information Center

Frazier Varner, Debrah

2010-01-01

Many adult basic education (ABE) programs do not achieve a high success rate in meeting student academic needs. Rooted in Knowles' theory of andragogy and Bandura's theory of modeling, this quantitative causal comparative study examined the effects of individualized instruction (IGI) and of facilitated, participatory group programs (SPOKES) on the…

WIOA: Implications for Low-Scoring Adult Learners

ERIC Educational Resources Information Center

Pickard, Amy

2016-01-01

A substantial proportion of participants in public adult education programs struggle with "basic" academic print literacy skills. According to the 2014-2015 National Reporting System (NRS) data, 48.7% of the national adult basic education/adult secondary education (ABE/ ASE) population tested as reading at or below the "Low…

Mobility of Adult Basic Education Students in BC: The 2004-05 Cohort

ERIC Educational Resources Information Center

Lawrance, Jill

2008-01-01

British Columbia offers multiple educational pathways to address the literacy and academic needs of its adult population. In BC, Adult Basic Education (ABE) is offered through two public systems: the secondary system offers the Adult Graduation program in various school district facilities, typically through Continuing Education, and the public…

Supervision Practices and Employee Relations in Adult Basic Education

ERIC Educational Resources Information Center

Even, Mary Jane

1977-01-01

Results of a study of supervisory practices among administrators of adult basic education (ABE) programs in Nebraska are reported. Highly significant associations were found between supervisory practices and employee attitudes toward their work, longevity, interest in promotion, productivity, and salary, among others. (TA)

Exploring Motivational System Theory within the Context of Adult Education

ERIC Educational Resources Information Center

Hutto, Debra Jean

2013-01-01

Adult Basic Education (ABE) and the General Equivalency Diploma (GED) programs serve those students who, for whatever reason, have left the educational system without attaining a regular high school diploma. Because of the manner in which they may have left the school system, many have negative emotions and personal agency beliefs hindering their…

Defining Success in Adult Basic Education Settings: Multiple Stakeholders, Multiple Perspectives

ERIC Educational Resources Information Center

Tighe, Elizabeth L.; Barnes, Adrienne E.; Connor, Carol M.; Steadman, Sharilyn C.

2013-01-01

This study employed quantitative and qualitative research approaches to investigate what constitutes success in adult basic education (ABE) programs from the perspectives of multiple educational stakeholders: the state funding agency, the teachers, and the students. Success was defined in multiple ways. In the quantitative section of the study, we…

Screening for Learning Disabilities in Adult Basic Education Students

ERIC Educational Resources Information Center

Reynolds, Sharon L.; Johnson, Jerry D.; Salzman, James A.

2012-01-01

The extant literature offers little to describe the processes for screening students in adult basic education (ABE) programs for potential learning disabilities, referring adult students for diagnostic assessment, or barriers to obtaining diagnostic assessment for a learning disability. Without current documentation of a learning disability, ABE…

The Joliet Junior College Center for Adult Basic Education and Literacy's "Families about Success": Intergenerational Programming That Works.

ERIC Educational Resources Information Center

Irby, Terry R.; And Others

Joliet Junior College's Center for Adult Basic Education and Literacy (CABEL) is responsible for providing adult basic education and support services for adults whose skills in reading and mathematics are below the 12th grade level. Its offerings include instruction in Adult Basic Education (ABE), General Educational Development (GED), English as…

Library-ABE Projects. Case Studies.

ERIC Educational Resources Information Center

MacVicar, Phyllis

This document contains 41 case studies submitted to the Appalachian Adult Education Center by the staffs of four projects demonstrating library services to disadvantaged adults, in cooperation with adult basic education programs. Included in each case study is the coping skill area in which an individual need was recognized and met through the…

Expanding Our Horizons. 1995 Success Stories.

ERIC Educational Resources Information Center

Royce and Royce, Inc., Lancaster, PA.

This booklet profiles 10 former participants in adult basic education (ABE) and literacy programs who have gone on to build successful lives and careers and have been designated outstanding adult students by the Pennsylvania Association for Adult and Continuing Education. The men and women selected as award winners were sponsored by the following…

Becoming Adult Learners: Principles and Practices for Effective Development

ERIC Educational Resources Information Center

Drago-Severson, Eleanor

2004-01-01

This book offers a new and promising way to support adults in Adult Basic Education (ABE) and English for speakers of other languages (ESOL) programs specifically, and learners in adult education, in general. Applying renowned Harvard University psychologist Robert Kegan's constructive-development theory, Drago-Severson depicts an in-depth…

The ABE Learner: Health, Learning Ability, Language and Communication.

ERIC Educational Resources Information Center

Glass, J. Conrad, Jr.; Hoffman, Lee McGraw

Intended to provide the teacher of adult basic education (ABE) with information about ABE learners, this handbook gives a picture of the more important characteristics which may influence the learning style and ability of ABE students. Practical suggestions are offered as to how the teacher may account for these characteristics in the…

Being a (Good) Student: Conceptions of Identity of Adult Basic Education Participants Transitioning to College

ERIC Educational Resources Information Center

Reddy, Mina

2012-01-01

This study examines the perceptions of identity of a category of students that has rarely been studied in the context of higher education. These are adults who have participated in GED preparation or English for Speakers of Other Languages (ESOL) courses in Adult Basic Education (ABE) programs. A college education is increasingly necessary for…

Adult Basic Education and Self-Esteem: Practical Strategies for Addressing Self-Esteem Problems among Basic Skills Students.

ERIC Educational Resources Information Center

Kirstein, Kurt D.

The strategies used by practicing adult basic education (ABE) teachers to retain students with poor self-esteem were examined through an Internet survey that was sent to 115 ABE instructors at community colleges in Washington. The survey, which contained questions about the prevalence of poor self-esteem among ABE dropouts, specific behaviors…

Causes for Attrition Among Adult Basic Education Students.

ERIC Educational Resources Information Center

Cramer, Patricia L.

This study was conducted to determine causes for attrition among students in an Adult Basic Education (A.B.E.) program located in a non-metropolitan area. Dropouts and completers were compared by demographic characteristics and by sources of difficulty (nonschool-related, school-related, and affective) in attending class. In addition, causes for…

Downtown Study Centre: An Open-Ended ABE Program in an Urban Shopping Mall

ERIC Educational Resources Information Center

Harrison, David

1976-01-01

Describes an adult basic education facility after one year of operation in an urban shopping mall in Nanaimo, British Columbia, Canada. The center is an informal open-ended classroom and advising center where part-time adult learners study an individualized curriculum. Summarizes the major findings of a program evaluation. (EM)

An Institute to Prepare Local Urban Adult Basic Education Administrators and Teachers to Become ABE Teacher Trainers: June 1, 1971-May 31, 1972. Final Report.

ERIC Educational Resources Information Center

Pumerantz, Phillip

The 12-month Federally funded project at the University of Bridgeport, Connecticut was designed to prepare adult basic education (ABE) administrators and teachers (serving urban Puerto Ricans, blacks, and whites) to become teacher trainers. Focus was on building a multi-regional teacher capability in ABE through teacher training models. Phase one…

Application of the Virginia STEPS (Student/Teacher Education Planning System) by Basic Skills Education Teachers.

ERIC Educational Resources Information Center

Seguin, Barbara; Swanson, Lois

The Virginia STEPS (Student/Teacher Education Planning System) was developed to enable adult basic education (ABE) students to become independent learners responsible for planning, carrying out, evaluating, and making adjustments in their education. ABE instructors at Blackhawk Technical College in Wisconsin have adapted the STEPS model to make…

Demonstration, Developmental and Research Project for Programs, Materials, Facilities and Educational Technology for Undereducated Adults: Ohio State Module. ABE Life-Centered Curriculum Development and Teaching Technique. Final Report.

ERIC Educational Resources Information Center

Morehead State Univ., KY.

Three types of instruction were used in the Ohio Module Project: traditional classes, programmed learning centers, and home instruction. Four major objectives of the project are: (1) to determine the kind of training program necessary to prepare paraprofessionals to operate an instructional program utilizing programmed materials, (2) to compare…

Employers' Perceptions of Basic Technology Skills Needed for Workplace Preparation in Adult Basic Education

ERIC Educational Resources Information Center

Yow, Alma V.

2010-01-01

Research has documented that many new entrants to the workforce from adult basic education (ABE) programs are critically lacking in the preparation and technology skills needed for workplace success. To address this problem, this basic interpretive qualitative study was implemented to examine and identify the basic technology skills perceived by…

Take School Out of ABE

ERIC Educational Resources Information Center

Mocker, Donald

1978-01-01

The author examines some of the barriers to learning found in public schools and concludes with an alternative which has the potential of providing a different philosophic basis for adult basic education programs, a literacy technique developed and implemented by Paulo Freire in Brazil. (MF)

Discovering New Hope through ABE: A Program for Kidney Dialysis Patients.

ERIC Educational Resources Information Center

Amonette, Linda M.

1984-01-01

Kidney dialysis patients often suffer emotional problems and face life adjustment problems. Adult basic education can be a useful tool to address these and to make positive use of idle time during dialysis. This article describes such a program, emphasizes the self-concept gain for students, and highlights the critical role of the understanding…

Organizational Culture in Adult Basic Education in the Netherlands.

ERIC Educational Resources Information Center

Berg, M. J. M. van den

Recent legislation on adult basic education (ABE) in the Netherlands has two important aims: to offer one uniform organizational financial structure and to improve educational quality. ABE institutes have concentrated on restructuring the organization and merging the precursors. The change in structure is supported by a change in organizational…

Creating In-Sync Environments for Children with Sensory Issues

ERIC Educational Resources Information Center

Humphries, Jane; Rains, Kari

2012-01-01

When Abe joined the program as an infant, the teachers in the classroom began to notice issues that made caring for Abe just a little more sensitive. As Abe grew into a mobile toddler, he would cover his ears when classroom noise got loud, cry when other children touched him, and often hide under tables to get away. Luckily for Abe, the staff and…

California Adult Education End-of-Year Progress Report to the Legislature: Implementation of the Workforce Investment Act (WIA) Title II. Program Year 2009. July 1, 2008 to June 30, 2009

ERIC Educational Resources Information Center

CASAS - Comprehensive Adult Student Assessment Systems (NJ1), 2010

2010-01-01

The Federal Workforce Investment Act (WIA) Title II, Adult Education and Family Literacy Act provides funding for states and territories to provide instruction in English as a Second Language (ESL), Adult Basic Education (ABE), and Adult Secondary Education (ASE) to adults in need of these literacy services. California State Budget Act language…

California Adult Education End-of-Year Progress Report to the Legislature: Implementation of the Workforce Investment Act (WIA) Title II. Program Year 2008. July 1, 2007 to June 30, 2008

ERIC Educational Resources Information Center

CASAS - Comprehensive Adult Student Assessment Systems (NJ1), 2009

2009-01-01

The Federal Workforce Investment Act (WIA) Title II, Adult Education and Family Literacy Act provides funding for states and territories to provide instruction in English as a Second Language (ESL), Adult Basic Education (ABE), and Adult Secondary Education (ASE) to adults in need of these literacy services. California State Budget Act language…

Examining Some Aspects of Alternative Basic Education Programmes in Ethiopia

ERIC Educational Resources Information Center

Onwu, Gilbert O. M.; Agu, Augustine

2010-01-01

This study examines some aspects of the quality of Alternative Basic Education (ABE) provision in Ethiopia. Educational indicators of quality were formulated under two general topic areas of ABE programme process and content, and pupil learning outcomes. A qualitative-interpretative research approach and survey design was used to collect data from…

Strategies for Success. A Study on ABE Student Retention.

ERIC Educational Resources Information Center

Jackson-Mayer, Sheila; And Others

This handbook is intended as a practical guide for adult basic education (ABE) directors, counselors, and faculty in an effort to increase ABE student retention. Following a literature review and description of the research problem, part I contains data obtained from the four major surveys administered during a project that investigated the causes…

Use of Authentic Assessment with ABE Students.

ERIC Educational Resources Information Center

Gall, Mary Louise; And Others

This report describes a project that developed a formal process and the accompanying instruments for authentic assessment of the academic and life skills of adult basic education (ABE) students. The assessment was used to assess the skills of 50 ABE students. The students and teacher evaluated the work that was done, the results were compiled, a…

Literacy Materials Bulletin 9-16.

ERIC Educational Resources Information Center

Literacy Materials Bulletin, 1997

1997-01-01

These eight bulletins, which are intended for literacy instructors, tutors, librarians, and others, contain reviews of literacy materials designed for use in adult literacy, adult basic education (ABE), and family literacy programs. Each review contains some or all of the following: title, author, publisher of the item being reviewed; information…

Dynamic Indicators of Basic Early Literacy Skills: An Effective Tool to Assess Adult Literacy Students?

ERIC Educational Resources Information Center

Binder, Katherine S.; Snyder, Melissa A.; Ardoin, Scott P.; Morris, Robin K.

2011-01-01

This study examined the reliability and validity of administering Dynamic Indicators of Basic Early Literacy Skills (DIBELS) to adult basic education (ABE) students. Ninety ABE participants were administered DIBELS measures, the Woodcock-Johnson III Broad Reading (WJ III BR) measures, and four orthographic ability tests. Since ABE students are a…

Reasons for Nonparticipation among Iowa Adults Who Are Eligible for ABE.

ERIC Educational Resources Information Center

Beder, Hal

A study was conducted in Iowa to determine why adults eligible for adult basic education (ABE) frequently fail to participate. The study was conducted on a representative sample of 129 persons who had not completed high school, were aged 18 or older, and had not attended ABE classes, through open-ended questions refined into telephone interview…

Recruiting Strategy. 310 Project.

ERIC Educational Resources Information Center

Stoll, Shirley

This five-part report describes a project to build community awareness of adult basic education (ABE) programs and their benefits in the Hawkeye Institute of Technology (HIT) service area. Part I reveals that the project's primary goal was to direct innovative recruitment activities toward undereducated adults in nine Iowa communities. It explains…

The Instructional Guide for Abbott Skills Enhancement Classes. Revised Edition.

ERIC Educational Resources Information Center

Ballinger, Ronda; Gee, Mary Kay

This guide, which integrates adult basic education (ABE) curriculum, job skills for Abbott Laboratories, and work-related foundation skills, is designed for an instructional program in the skill areas of reading, writing, oral communications, mathematics, and problem solving. In addition to creating a uniform process and product to promote…

Pillars of Support: A Functional Asset-Based Framework for ABE Learners

ERIC Educational Resources Information Center

Reynolds, Sharon; Johnson, Jerry

2014-01-01

This paper reported results from a qualitative analysis of assets and supports disclosed in the narratives of adult basic education students. These students were identified as exemplary by their instructors for academic achievement, hours of program attendance, or community service. Themes were identified using the "Four Pillars"…

Project MAPP. The Vocabulary Booklet # 1. Implementing the Instructional Program: A Competency-Based Adult Education Learning System.

ERIC Educational Resources Information Center

Maryland State Dept. of Education, Baltimore.

This booklet is intended to help adults master the basic and life skill vocabulary needed to meet the simple communication demands of daily life. It is designed to assist adult basic education (ABE) teachers in the implementation of a competency-based learning system that emphasizes the integration of basic and life skills learning. The booklet…

The University of Missouri Adult Basic Education System.

ERIC Educational Resources Information Center

Mocker, Donald W.; Veri, Clive C.

The University of Missouri adult basic education (ABE) system, developed from the ABE laboratory with the help of Title III funds, consists of a series of subsystems designed to effect behavioral change in undereducated adults through an individualized flexible approach to learning. The subsystems of diagnostic testing; individual counseling,…

Dropout and Persistence in Adult Basic Education.

ERIC Educational Resources Information Center

Clark, Lisa K.

A review of research on dropouts from adult basic education (ABE) reveals several theoretical models relevant to the study of dropout and persistence in ABE, shows specific variables related to dropout behavior, provides insights as to who drops out and why, identifies some characteristics of disadvantaged adult learners, and reveals some problems…

Maintaining "Professional Distance": A Dilemma for the ABE Teacher.

ERIC Educational Resources Information Center

Willing, Delight C.; Haney, Hutch

1994-01-01

Too often, many adult educators are unaware of the problems resulting from failing to maintain an appropriate "professional distance" from their adult basic education (ABE) or English-as-a-Second-Language (ESL) students when those students encounter personal problems or crises that appear to be interfering with their learning. ABE…

Health Literacy and Adult Basic Education Assessments

ERIC Educational Resources Information Center

Golbeck, Amanda L.; Ahlers-Schmidt, Carolyn R.; Paschal, Angelia M.

2005-01-01

Adult basic education (ABE) is an ideal venue for developing health literacy skills. Literacy and numeracy assessments used in ABE were identified and the most common were examined for health components. Only the Comprehensive Adult Student Assessment System (CASAS) included health. The two most common health literacy assessments used in general…

Using Modern Statistical Methods to Analyze Demographics of Kansas ABE/GED Students Who Transition to Community or Technical College Programs

ERIC Educational Resources Information Center

Zacharakis, Jeff; Wang, Haiyan; Patterson, Margaret Becker; Andersen, Lori

2015-01-01

This research analyzed linked high-quality state data from K-12, adult education, and postsecondary state datasets in order to better understand the association between student demographics and successful completion of a postsecondary program. Due to the relatively small sample size compared to the large number of features, we analyzed the data…

Achievement Motivation Training--Effects on ABE/ASE Students' Psychosocial Self-Perceptions.

ERIC Educational Resources Information Center

Martin, Larry G.

A study was conducted to identify psychosocial needs of Adult Basic Education (ABE)/Adult Secondary Education (ASE) students by using the Self-Description Questionnaire (SDQ). A second purpose was to test effectiveness of Achievement Motivation Training (AMT) as a technique to counterbalance the negative impact of these students' former…

Adult Basic Education Basic Computer Literacy Handbook.

ERIC Educational Resources Information Center

Manini, Catalina M.; Cervantes, Juan

This handbook, in both English and Spanish versions, is intended for use with adult basic education (ABE) students. It contains five sections of basic computer literacy activities and information about the ABE computer literacy course offered at Dona Ana Community College (DACC) in New Mexico. The handbook begins with forewords by the handbook's…

Personality Characteristics and Learning Style Preferences of Adult Basic Education Students. Research Monograph.

ERIC Educational Resources Information Center

Manzo, Anthony V.; And Others

The study described in the report identifies personality characteristics and learning styles of adult basic education (ABE) students on the basis of three instruments: the Luscher Color Test, the Manzo Bestiary Inventory, and the Learning Preference Inventory. The volunteer sample consisted of 83 ABE students. Subsample comparison groups consisted…

Massachusetts Adult Basic Education Curriculum Framework for Mathematics and Numeracy

ERIC Educational Resources Information Center

Massachusetts Department of Education, 2005

2005-01-01

Over the past number of years, several initiatives have set the stage for writing the Massachusetts ABE (Adult Basic Education) Curriculum Frameworks for Mathematics and Numeracy. This current version of the "Massachusetts ABE Mathematics Curriculum Frameworks" is a second revision of that first framework, but it is heavily influenced by…

National Issues Forums in an ABE Setting. Final Report.

ERIC Educational Resources Information Center

Molek, Carol

National Issues Forums (NIFs) were conducted for adult basic education (ABE) students in a Pennsylvania adult education and job training center. The forums provide a process of sharing thoughts and opinions about areas of pressing national concerns in an open exchange of everyone's opinion. After instructors participated in NIFs, they developed a…

Does Prison-Based Adult Basic Education Improve Postrelease Outcomes for Male Prisoners in Florida?

ERIC Educational Resources Information Center

Cho, Rosa Minhyo; Tyler, John H.

2013-01-01

The authors use administrative data from Florida to determine the extent to which prison-based adult basic education (ABE) improves inmate's postrelease labor market outcomes, such as earnings and employment. Using two nonexperimental comparison groups, the authors find evidence that ABE participation is associated with higher postrelease earnings…

Correlation Study of Adult English as a Second Language (ESL) and Adult Basic Education (ABE) Reading Tests. Final Report.

ERIC Educational Resources Information Center

Garreton, Rodrigo; Terdy, Dennis

In a study prompted by the need to standardize the reporting of educational progress of adult language minority students in Illinois, a commonly used adult English-as-a-Second-Language (ESL) reading test was compared with two frequently used Adult Basic Education (ABE) reading tests. The testing instruments used were the ELSA (English Language…

The Delivery and Content of Training for Adult Education Teachers and Volunteer Instructors. Study of ABE/ESL Instructor Training Approaches.

ERIC Educational Resources Information Center

Tibbetts, John; And Others

The delivery and content of training for teachers and volunteer instructors in adult basic education (ABE) and English as a Second Language (ESL) were documented in a study. Two sources of information were the research literature and extant data from states on training activities. Findings indicated that training for adult education teachers and…

Cooperative Program Improvement; An Experiment in ABE In-Service Training. Doolittle Family Education Center Experimental In-Service Training Project. Final Report.

ERIC Educational Resources Information Center

Griffith, William S.; And Others

This document, consisting of seven chapters and 12 appendixes, is a full final report of the Doolittle Family Education Center Experimental In-Service Training Project. Chapter II consists of the history and plan of the project including an explanation of the framework of the model that was used to conceptualize the project. Chapter III is a…

Wyoming Strategic Plan, 2005

ERIC Educational Resources Information Center

Wyoming Community College Commission, 2005

2005-01-01

Wyoming's colleges offer much more than academic and occupational technical degrees and certificates. In 2000, 27,703 Wyoming citizens, age 25 years and older, did not have a high school diploma. For this 12.14% of Wyoming's population, the Adult Basic Education (ABE) program at each of the colleges is designed to equip these adults with the…

Entrepreneurial Training Needs of Illiterate Women in Cross River State, Nigeria

ERIC Educational Resources Information Center

Ingwu, Emmanuel U.; Okey, Stella-Maris A.

2013-01-01

In order to improve on the curriculum and participation rate of adult learners in the current Adult Basic Education (ABE) program in Nigeria, this explorative study investigated the entrepreneurial (or vocational) training needs of illiterate women in Cross River State (CRS). Three research questions were posed to elicit from the participants…

Adult Basic Education in British Columbia's Public Post-Secondary Institutions. An Articulation Handbook. 2007-2008 Edition

ERIC Educational Resources Information Center

Ministry of Advanced Education, 2007

2007-01-01

This is the twenty-second edition of the Adult Basic Education (ABE) Articulation Handbook for British Columbia's public postsecondary institutions. The purpose of ABE articulation is to ensure learners have access to quality courses, receive appropriate credits and are able to transfer easily among publicly-funded colleges, university colleges,…

Exploring the Dimensionality of Morphological Awareness and Its Relations to Vocabulary Knowledge in Adult Basic Education Students

ERIC Educational Resources Information Center

Tighe, Elizabeth L.; Schatschneider, Christopher

2015-01-01

The purpose of this study was to examine the construct of morphological awareness and its relations to vocabulary knowledge in Adult Basic Education (ABE) students. Morphological awareness has emerged as an important predictor of children's and ABE students' reading comprehension abilities; however, there has been a dearth of research…

Using the ICOT Instrument to Improve Instructional Technology Usage in the ABE Classroom

ERIC Educational Resources Information Center

Lentz, Brannon W.

2011-01-01

The International Society for Technology (ISTE) in Education promotes the use of a specific tool--the ISTE Classroom Observation Tool (ICOT)--to measure and improve the use of instructional technologies in Adult Basic Education (ABE) classrooms. The purpose of this article is to describe an application process for the use of the ICOT instrument…

Demonstration, Developmental and Research Project for Programs, Materials, Facilities and Educational Technology for Undereducated Adults: Ohio State Module. ABE Life-Centered Curriculum Development and Teaching Technique. Final Report.

ERIC Educational Resources Information Center

Morehead State Univ., KY.

This workshop was held for the purpose of training selected staff members of the Ohio Module Field Unit of the Appalachian Adult Basic Education Demonstration Center (AABEDC). Twelve persons, six teachers and six paraprofessionals, were selected to participate in the workshop. While their specific jobs vary, all will be concerned with utilization…

The Contributions of Phonological and Morphological Awareness to Literacy Skills in the Adult Basic Education Population

ERIC Educational Resources Information Center

Fracasso, Lucille E.; Bangs, Kathryn; Binder, Katherine S.

2016-01-01

The Adult Basic Education (ABE) population consists of a wide range of abilities with needs that may be unique to this set of learners. The purpose of this study was to better understand the relative contributions of phonological decoding and morphological awareness to spelling, vocabulary, and comprehension across a sample of ABE students. In…

An Investigation of the Factors That Motivate Adults to Participate in Adult Basic Education (ABE) Classes at a Southeastern Wisconsin Community College

ERIC Educational Resources Information Center

Crump-Phillips, Maureen R.

2013-01-01

This study assessed the plausibility of using Ajzen's (1991) theory of planned behavior (TPB) to identify the factors that motivate adults to participate in Adult Basic Education (ABE) classes at a Southeast Wisconsin Community College. The original TPB (Ajzen, 1991) attests that planned behaviors are determined by behavioral intentions which are…

Volunteer English as a Second Language Instructional Program for Non-English Speaking Adults. Final Report.

ERIC Educational Resources Information Center

Catholic Social Services, Harrisburg, PA.

The primary goal of a multi-purpose project was to utilize both Literacy Volunteers of America (LVA) and Laubach Literacy Action (LLA) in training volunteers to teach English to refugees. Catholic Social Services trained 163 volunteers who were placed in adult basic education (ABE) classes, small group instruction settings, and one-to-one tutoring…

Challenges and Solutions to Assisting Older Adults in Completing the GED: A Study of What the Experts Say

ERIC Educational Resources Information Center

Brinkley-Etzkorn, Karen E.

2016-01-01

The purpose of this study was to investigate the challenges and solutions encountered by Adult Basic Education (ABE) programs currently serving older adults seeking a GED credential in states where this is the only high school equivalency option available. The following questions guided this research: (1) what are the perceived characteristics and…

Annotated A.B.E. Bibliography.

ERIC Educational Resources Information Center

Anderson, Ethel E., Comp.

Based on lists submitted by practitioners working in the 0 to grade 9 levels of English-speaking adult basic education (ABE) in Canada, this annotated bibliography is composed of 283 items currently in use. Six categories are included: (1) reading, which covers reading systems, instructional material, phonics, and independent reading; (2) language…

Learned Helplessness and Dyslexia: A Carts and Horses Issue?

ERIC Educational Resources Information Center

Kerr, H.

2001-01-01

Surveys attitudes towards and beliefs about dyslexia among Adult Basic Education (ABE) teachers and providers. Finds doubt, uncertainty and confusion about dyslexia and considerable misgiving. Discusses attribution theory and learned helplessness in the context of ABE. Argues that a diagnosis of dyslexia may be a maladaptive attribution and so…

Teaching as a Social Practice: The Experiences of Two Moroccan Adult Literacy Tutors

ERIC Educational Resources Information Center

Erguig, Reddard

2012-01-01

This article offers an ethnographic case study of two Adult Basic Education (ABE) teachers' characteristics and their literacy instruction. It draws on the New Literacy Studies tradition and used ethnographic tools (in-depth interviews, classroom observation and the think-aloud protocol) to explore the characteristics of two ABE teachers and…

A Quantile Regression Approach to Understanding the Relations Between Morphological Awareness, Vocabulary, and Reading Comprehension in Adult Basic Education Students

PubMed Central

Tighe, Elizabeth L.; Schatschneider, Christopher

2015-01-01

The purpose of this study was to investigate the joint and unique contributions of morphological awareness and vocabulary knowledge at five reading comprehension levels in Adult Basic Education (ABE) students. We introduce the statistical technique of multiple quantile regression, which enabled us to assess the predictive utility of morphological awareness and vocabulary knowledge at multiple points (quantiles) along the continuous distribution of reading comprehension. To demonstrate the efficacy of our multiple quantile regression analysis, we compared and contrasted our results with a traditional multiple regression analytic approach. Our results indicated that morphological awareness and vocabulary knowledge accounted for a large portion of the variance (82-95%) in reading comprehension skills across all quantiles. Morphological awareness exhibited the greatest unique predictive ability at lower levels of reading comprehension whereas vocabulary knowledge exhibited the greatest unique predictive ability at higher levels of reading comprehension. These results indicate the utility of using multiple quantile regression to assess trajectories of component skills across multiple levels of reading comprehension. The implications of our findings for ABE programs are discussed. PMID:25351773

Where We Live: A Curriculum Guide. ABE Materials that Address Housing Issues.

ERIC Educational Resources Information Center

Ellowitch, Azi

This curriculum was developed to give adult basic education (ABE) teachers starting points for developing their own units around housing-related issues. The texts have been chosen thematically, rather than by skill level. The materials are designed for group work--oral reading and discussion. Readings focus on housing repairs, court procedures,…

The Learner Persistence Project at Quinsigamond Community College

ERIC Educational Resources Information Center

Kefallinou, Maria

2009-01-01

Quinsigamond Community College's (QCC's) Adult Community Learning Center is located in Worcester, Massachusetts, and offers ESOL and ABE/GED classes to students who reside in Worcester and the surrounding towns (urban area). Its ABE/GED program has historically had low retention and persistence. Students often left before the end of the year or…

Tutor Training Packet. "Ready-Set-ABE" To Ease Students' Transition into ABE Level Studies.

ERIC Educational Resources Information Center

Molek, Carol

This training packet, consisting of a workshop guide, two instructional guides, and assorted pamphlets and brochures, is intended for use by volunteer tutors who are themselves learning how to work with adults enrolled in an adult literacy program. The following topics are covered in the training workshop guide: the objectives and workings of…

Project on Teaching Charts and Graphs to ABE Students. Part I: Teacher's Guide [and] Part II: Transparency Assembly Package.

ERIC Educational Resources Information Center

Renton Vocational Inst., WA.

The teacher's guide and collection of transparency masters are designed for use in teaching adult basic education (ABE) students how to read and interpret graphs and charts. Covered in the individual lessons of the instructional unit are the reading and interpretation of charts as well as picture, line, bar, and circle graphs. Each unit contains a…

Why Understanding 1-3/4 divided by 1/2 Matters to Math Reform: ABE Teachers Learn the Math They Teach.

ERIC Educational Resources Information Center

Brover, Charles; Deagan, Denise; Farina, Solange

This paper explains the investigative attempts of The New York City Math Exchange Group (MEG) on elementary mathematics teachers' content knowledge in Adult Basic Education (ABE). The study is comparative in nature and took place in a workshop at the Adults Learning Maths Conference in Boston. The new members of the MEG professional development…

Learning with Pictures, Signs and Symbols (A Language Arts and Consumer Mathematics Curriculum for the 0-4 Level ABE Student). Final Report.

ERIC Educational Resources Information Center

Robinson, Nancy; Selkirk, Betty

The ARIN Adult Learning Center in Indiana, Pennsylvania conducted a project to develop language arts and consumer mathematics curriculums for O-4 level adult basic education (ABE) students. Using the five knowledge areas of the adult performance levels as established by the University of Texas, (consumer economics, health, occupational knowledge,…

The AstroBiology Explorer (ABE) MIDEX Mission Concept: Using Infrared Spectroscopy to Identify Organic Molecules in Space

NASA Technical Reports Server (NTRS)

Sandford, Scott A.; Ennico, Kimberly; Allamandola, Louis; Bregman, Jesse; Greene, Thomas; Hudgins, Douglas

2002-01-01

One of the principal means by which organic compounds are detected and identified in space is by infrared spectroscopy. Past IR telescopic and laboratory studies have shown that much of the carbon in the interstellar medium (ISM) is in complex organic species but the distribution, abundance and evolutionary relationships of these materials are not well understood. The Astrobiology Explorer (ABE) is a MIDEX mission concept designed to conduct IR spectroscopic observations to detect and identify these materials and address outstanding problems in astrobiology, astrochemistry, and astrophysics. ABE's core science program includes observations of planetary nebulae and stellar outflows, protostellar objects, Solar System objects, and galaxies, and lines of sight through dense molecular clouds and the diffuse ISM. ABE is a cryogenically-cooled 60 cm diameter space telescope equipped with 3 cross-dispersed R-2000 spectrometers that share a single common slit. Each spectrometer measures one spectral octave and together cover the entire 2.5-20 micron region simultaneously. The spectrometers use state-of-the-art InSb and Si:As 1024x1024 pixel detectors. ABE would operate in a heliocentric, Earth drift-away orbit and have a core science mission lasting approximately 1.5 years. ABE is currently under study at NASA's Ames Research Center in collaboration with Ball Aerospace and Technologies Corp.

ABE Phase III: Progress and Problems. September 1, 1969-April 1, 1970.

ERIC Educational Resources Information Center

Southwestern Cooperative Educational Lab., Albuquerque, NM.

Interim information concerning the ABE III grants is provided in the three parts of this report. Part 1 (outline) describes the goals and objectives of each component; Part 2 describes accomplishments and problems to date; and Part 3 deals with coordination and supervision activities undertaken by the Lab. The components of the program are: (1)…

Audit-based education lowers systolic blood pressure in chronic kidney disease: the Quality Improvement in CKD (QICKD) trial results

PubMed Central

de Lusignana, Simon; Gallagher, Hugh; Jones, Simon; Chan, Tom; van Vlymen, Jeremy; Tahir, Aumran; Thomas, Nicola; Jain, Neerja; Dmitrieva, Olga; Rafi, Imran; McGovern, Andrew; Harris, Kevin

2013-01-01

Strict control of systolic blood pressure is known to slow progression of chronic kidney disease (CKD). Here we compared audit-based education (ABE) to guidelines and prompts or usual practice in lowering systolic blood pressure in people with CKD. This 2-year cluster randomized trial included 93 volunteer general practices randomized into three arms with 30 ABE practices, 32 with guidelines and prompts, and 31 usual practices. An intervention effect on the primary outcome, systolic blood pressure, was calculated using a multilevel model to predict changes after the intervention. The prevalence of CKD was 7.29% (41,183 of 565,016 patients) with all cardiovascular comorbidities more common in those with CKD. Our models showed that the systolic blood pressure was significantly lowered by 2.41 mm Hg (CI 0.59–4.29 mm Hg), in the ABE practices with an odds ratio of achieving at least a 5 mm Hg reduction in systolic blood pressure of 1.24 (CI 1.05–1.45). Practices exposed to guidelines and prompts produced no significant change compared to usual practice. Male gender, ABE, ischemic heart disease, and congestive heart failure were independently associated with a greater lowering of systolic blood pressure but the converse applied to hypertension and age over 75 years. There were no reports of harm. Thus, individuals receiving ABE are more likely to achieve a lower blood pressure than those receiving only usual practice. The findings should be interpreted with caution due to the wide confidence intervals. PMID:23536132

A Quantile Regression Approach to Understanding the Relations Among Morphological Awareness, Vocabulary, and Reading Comprehension in Adult Basic Education Students.

PubMed

Tighe, Elizabeth L; Schatschneider, Christopher

2016-07-01

The purpose of this study was to investigate the joint and unique contributions of morphological awareness and vocabulary knowledge at five reading comprehension levels in adult basic education (ABE) students. We introduce the statistical technique of multiple quantile regression, which enabled us to assess the predictive utility of morphological awareness and vocabulary knowledge at multiple points (quantiles) along the continuous distribution of reading comprehension. To demonstrate the efficacy of our multiple quantile regression analysis, we compared and contrasted our results with a traditional multiple regression analytic approach. Our results indicated that morphological awareness and vocabulary knowledge accounted for a large portion of the variance (82%-95%) in reading comprehension skills across all quantiles. Morphological awareness exhibited the greatest unique predictive ability at lower levels of reading comprehension whereas vocabulary knowledge exhibited the greatest unique predictive ability at higher levels of reading comprehension. These results indicate the utility of using multiple quantile regression to assess trajectories of component skills across multiple levels of reading comprehension. The implications of our findings for ABE programs are discussed. © Hammill Institute on Disabilities 2014.

AstroBiology Explorer (ABE) MIDEX mission concept

NASA Astrophysics Data System (ADS)

Ennico, Kimberly A.; Sandford, Scott; Cox, Sylvia; Ellis, Benton; Gallagher, Dennis J.; Gautier, Nick; Greene, Thomas P.; McCreight, Craig R.; Mills, Gary; Purcell, William R.

2002-02-01

The Astrobiology Explorer (ABE) is a MIDEX mission concept under study at NASA's Ames Research Center in collaboration with Ball Aerospace & Technologies, Corp. ABE will conduct IR spectroscopic observations to address important problems in astrobiology, astrochemistry, and astrophysics. The core observational program would make fundamental scientific progress in understanding the distribution, identity, and evolution of ices and organic matter in dense molecular clouds, young forming stellar systems, stellar outflows, the general diffuse ISM, HII regions, Solar System bodies, and external galaxies. The ABE instrument concept includes a 0.6 m aperture Cassegrain telescope and two moderate resolution (R equals 2000-3000) spectrographs covering the 2.5-16 micron spectral region. Large format (1024x1024 pixel or larger) IR detector arrays and bandpass filters will allow each spectrograph to cover an entire octave of spectral range or more per exposure without any moving parts. The telescope will be cooled below 50 K by a cryogenic dewar shielded by a sunshade. The detectors will be cooled to ~8K. The optimum orbital configuration for achieving the scientific objectives of the ABE mission is a low background, 1 AU Earth driftaway orbit requiring a Delta II launch vehicle. This configuration provides a low thermal background and allows adequate communications bandwidth and good access to the entire sky over the ~1-2 year mission lifetime.

The Contributions of Phonological and Morphological Awareness to Literacy Skills In the Adult Basic Education Population

PubMed Central

Fracasso, Lucille E.; Bangs, Kathryn; Binder, Katherine S.

2014-01-01

The Adult Basic Education (ABE) population consists of a wide range of abilities with needs that may be unique to this set of learners. The purpose of this study was to better understand the relative contributions of phonological decoding and morphological awareness to spelling, vocabulary, and comprehension across a sample of ABE students. In this study, phonological decoding was a unique predictor of spelling ability, listening comprehension and reading comprehension. We also found that morphological awareness was a unique predictor of spelling ability, vocabulary, and listening comprehension. Morphological awareness indirectly contributed to reading comprehension through vocabulary. These findings suggest the need for morphological interventions for this group of learners. PMID:24935886

Energy efficiency of acetone, butanol, and ethanol (ABE) recovery by heat-integrated distillation.

PubMed

Grisales Diaz, Victor Hugo; Olivar Tost, Gerard

2018-03-01

Acetone, butanol, and ethanol (ABE) is an alternative biofuel. However, the energy requirement of ABE recovery by distillation is considered elevated (> 15.2 MJ fuel/Kg-ABE), due to the low concentration of ABE from fermentation broths (between 15 and 30 g/l). In this work, to reduce the energy requirements of ABE recovery, four processes of heat-integrated distillation were proposed. The energy requirements and economic evaluations were performed using the fermentation broths of several biocatalysts. Energy requirements of the processes with four distillation columns and three distillation columns were similar (between 7.7 and 11.7 MJ fuel/kg-ABE). Double-effect system (DED) with four columns was the most economical process (0.12-0.16 $/kg-ABE). ABE recovery from dilute solutions by DED achieved energy requirements between 6.1 and 8.7 MJ fuel/kg-ABE. Vapor compression distillation (VCD) reached the lowest energy consumptions (between 4.7 and 7.3 MJ fuel/kg-ABE). Energy requirements for ABE recovery DED and VCD were lower than that for integrated reactors. The energy requirements of ABE production were between 1.3- and 2.0-fold higher than that for alternative biofuels (ethanol or isobutanol). However, the energy efficiency of ABE production was equivalent than that for ethanol and isobutanol (between 0.71 and 0.76) because of hydrogen production in ABE fermentation.

The AstroBiology Explorer (ABE) MIDEX Mission Concept: Identifying Organic Molecules in Space

NASA Astrophysics Data System (ADS)

Ennico, Kimberly A.; Sandford, Scott; Allamandola, Louis; Bregman, Jesse D.; Cohen, Martin; Cruikshank, Dale; Greene, Thomas P.; Hudgins, Douglas; Kwok, Sun; Lord, Steven D.; Madden, Suzanne; McCreight, Craig R.; Roellig, Thomas L.; Strecker, Donald W.; Tielens, A. G. G. M.; Werner, Michael W.

2003-03-01

The Astrobiology Explorer (ABE) is a MIDEX mission concept, currently under Concept Phase A study at NASA's Ames Research Center in collaboration with Ball Aerospace &Technologies, Corp., and managed by NASA's Jet Propulsion Laboratory. ABE will conduct infrared spectroscopic observations to address important problems in astrobiology, astrochemistry, and astrophysics. The core observational program would make fundamental scientific progress in understanding the distribution, identity, and evolution of ices and organic matter in dense molecular clouds, young forming stellar systems, stellar outflows, the general diffuse ISM, HII regions, Solar System bodies, and external galaxies. The ABE instrument concept includes a 0.6 m aperture Ritchey-Chretien telescope and three moderate resolution (R = 2000-3000) spectrometers together covering the 2.5-20 micron spectral region. Large format (1024 x 1024 pixel) IR detector arrays will allow each spectrometer to cover an entire octave of spectral range per exposure without any moving parts. The telescope will be cooled below 50 K by a cryogenic dewar shielded by a sunshade. The detectors will be cooled to ~7.5 K by a solid hydrogen cryostat. The optimum orbital configuration for achieving the scientific objectives of the ABE mission is a low background, 1 AU Earth driftaway orbit requiring a Delta II launch vehicle. This configuration provides a low thermal background and allows adequate communications bandwidth and good access to the entire sky over the ~1.5 year mission lifetime.

The Contributions of Phonological and Morphological Awareness to Literacy Skills in the Adult Basic Education Population.

PubMed

Fracasso, Lucille E; Bangs, Kathryn; Binder, Katherine S

2016-01-01

The Adult Basic Education (ABE) population consists of a wide range of abilities with needs that may be unique to this set of learners. The purpose of this study was to better understand the relative contributions of phonological decoding and morphological awareness to spelling, vocabulary, and comprehension across a sample of ABE students. In this study, phonological decoding was a unique predictor of spelling ability, listening comprehension, and reading comprehension. We also found that morphological awareness was a unique predictor of spelling ability, vocabulary, and listening comprehension. Morphological awareness indirectly contributed to reading comprehension through vocabulary. These findings suggest the need for morphological interventions for this group of learners. © Hammill Institute on Disabilities 2014.

The State of Continuing Education in Japan.

ERIC Educational Resources Information Center

Masatoshi, Nagashima, Ed.; And Others

This book contains the following articles about the state of continuing education and occupational training in Japan: "Funabashi City University of Sports Health" (Abe Nobuhiro); "Public Halls in Omiya City" (Kawamoto Koji); "Setagaya Senior Citizens College (Setagaya Rojin Daigaku)" (Sawamura Hiroshi); "Lifelong…

Policy to Performance Toolkit: Transitioning Adults to Opportunity

ERIC Educational Resources Information Center

Alamprese, Judith A.; Limardo, Chrys

2012-01-01

The "Policy to Performance Toolkit" is designed to provide state adult education staff and key stakeholders with guidance and tools to use in developing, implementing, and monitoring state policies and their associated practices that support an effective state adult basic education (ABE) to postsecondary education and training transition…

Suggestions for Realization Rather than Professionalization in Adult Basic Education.

ERIC Educational Resources Information Center

Allen, Fred

1987-01-01

A literature review shows lack of consensus in defining adult education and rejection of the application of the traditional model of professionalism to adult basic education (ABE). Carl Rogers' concept of substituting realization for professionalization, as well as the literature of staff preservice and inservice training, can help practitioners…

ABE Has Growing Pains

ERIC Educational Resources Information Center

Frank, Boris

1970-01-01

There are problems in adult basic education--little specially produced professional material available to it; little time spent on preplanning stages; severe budgetary limitations; and an elementary school syndrome. (EB)

Rocket propulsion research at Lewis Research Center

NASA Technical Reports Server (NTRS)

Dawson, Virginia P.

1992-01-01

A small contingent of engineers at NASA LeRC pioneered the basic research on liquid propellants for rockets shortly after World War 2. Carried on through the 1950s, this work influenced the important early decisions made by Abe Silverstein when he took charge of the Office of Space Flight Programs for NASA. He strongly supported the development of liquid hydrogen as a propulsion fuel in the face of resistance from Wernher von Braun. Members of the LeRC staff played an important role in bringing liquid hydrogen technology to the point of reliability through their management of the Centaur Program. This paper demonstrates how the personality and engineering intuition of Abe Silverstein shaped the Centaur program and left a lasting imprint on the laboratory research tradition. Many of the current leaders of LeRC received their first hands-on engineering experience when they worked on the Centaur program in the 1960s.

Rocket Propulsion Research at Lewis Research Center

NASA Technical Reports Server (NTRS)

Dawson, Virginia P.

1992-01-01

A small contingent of engineers at NASA Lewis Research Center pioneered in basic research on liquid propellants for rockets shortly after World War II. Carried on through the 1950s, this work influenced the important early decisions made by Abe Silverstein when he took charge of the Office of Space Flight Programs for NASA. He strongly supported the development of liquid hydrogen as a propulsion fuel in the face of resistance from Wernher von Braun. Members of the Lewis staff played an important role in bringing liquid hydrogen technology to the point of reliability through their management of the Centaur Program. This paper demonstrates how the personality and engineering intuition of Abe Silverstein shaped the Centaur program and left a lasting imprint on the laboratory research tradition. Many of the current leaders of Lewis Research Center received their first hands-on engineering experience when they worked on the Centaur program in the 1960s.

Competency-Based Adult Education Classroom Management Guide for Adult Basic Education Curriculum (Level II, 5-8).

ERIC Educational Resources Information Center

Singer, Elizabeth

This Competency-Based Adult Basic Education (CBABE) Classroom Management Guide was developed to aid the Adult Basic Education (ABE) facilitator in implementing a model CBABE Level 5-8 curriculum. First, introductory material provides background on the CBABE project at Brevard Community College (Florida) and the rationale for the development of the…

Canadian Association for the Study of Adult Education. Proceedings of the Annual Conference (3rd, Guelph, Ontario, Canada, June 8-10, 1984).

ERIC Educational Resources Information Center

Bartram, Peter E., Ed.

These proceedings contain 24 papers in English and 3 papers in French: "Adult Education and the Social Sciences" (Draper); "Readability as Applied to an ABE (Adult Basic Education) Assessment Instrument" (Taylor, Wahlstrom); "Quality of Worklife: Adult Education Administrators Speak Out" (McKee, Murphy); "The…

The AstroBiology Explorer (ABE) MIDEX Mission Concept: Identifying Organic Molecules in Space

NASA Technical Reports Server (NTRS)

Ennico, Kimberly; Sandford, Scott; Allamandola, Louis; Bregman, Jesse; Cohen, Martin; Cruikshank, Dale; Greene, Thomas; Hudgins, Douglas; Kwok, Sun; Lord, Steven;

Investigating Predictors of Spelling Ability for Adults with Low Literacy Skills

PubMed Central

Talwar, Amani; Cote, Nicole Gilbert; Binder, Katherine S.

2014-01-01

This study examined whether the spelling abilities of adults with low literacy skills could be predicted by their phonological, orthographic, and morphological awareness. Sixty Adult Basic Education (ABE) students completed several literacy tasks. It was predicted that scores on phonological and orthographic tasks would explain variance in spelling scores, whereas scores on morphological tasks may not. Scores on all phonological tasks and on one orthographic task emerged as significant predictors of spelling scores. Additionally, error analyses revealed a limited influence of morphological knowledge in spelling attempts. Implications for ABE instruction are discussed. PMID:25364644

Developing Distance Education. Papers Submitted to the World Conference of the International Council for Distance Education (14th, Oslo, Norway, August 9-16, 1988).

ERIC Educational Resources Information Center

Sewart, David, Ed.; Daniel, John S., Ed.

These proceedings contain 10 keynote papers and more than 100 papers from an international conference on the theme of developing distance education. The keynote papers are: (1) "Communications Technology" (Yoshia Abe); (2) "Continuing Education. New Needs and Challenges for Distance Studies" (Urban Dahllof); (3) "Distance…

AECAP Guide for State Leaders: State and Local Coordination and Planning to Strengthen Adult Basic Education Services

ERIC Educational Resources Information Center

Alamprese, Judith A.; Gwaltney, Margaret K.

2010-01-01

The Adult Education Coordination and Planning (AECAP) guide is designed to assist state adult education staff in forming partnerships at the state level and facilitating coordination at the local level as a lever for expanding and improving the quality of adult basic education (ABE) and workforce development services. Coordination plays a critical…

A Survey of Research Progress and Development Tendency of Attribute-Based Encryption

PubMed Central

Pang, Liaojun; Yang, Jie; Jiang, Zhengtao

2014-01-01

With the development of cryptography, the attribute-based encryption (ABE) draws widespread attention of the researchers in recent years. The ABE scheme, which belongs to the public key encryption mechanism, takes attributes as public key and associates them with the ciphertext or the user's secret key. It is an efficient way to solve open problems in access control scenarios, for example, how to provide data confidentiality and expressive access control at the same time. In this paper, we survey the basic ABE scheme and its two variants: the key-policy ABE (KP-ABE) scheme and the ciphertext-policy ABE (CP-ABE) scheme. We also pay attention to other researches relating to the ABE schemes, including multiauthority, user/attribute revocation, accountability, and proxy reencryption, with an extensive comparison of their functionality and performance. Finally, possible future works and some conclusions are pointed out. PMID:25101313

Enhancing clostridial acetone-butanol-ethanol (ABE) production and improving fuel properties of ABE-enriched biodiesel by extractive fermentation with biodiesel.

PubMed

Li, Qing; Cai, Hao; Hao, Bo; Zhang, Congling; Yu, Ziniu; Zhou, Shengde; Chenjuan, Liu

2010-12-01

The extractive acetone-butanol-ethanol (ABE) fermentations of Clostridium acetobutylicum were evaluated using biodiesel as the in situ extractant. The biodiesel preferentially extracted butanol, minimized product inhibition, and increased production of butanol (from 11.6 to 16.5 g L⁻¹) and total solvents (from 20.0 to 29.9 g L⁻¹) by 42% and 50%, respectively. The fuel properties of the ABE-enriched biodiesel obtained from the extractive fermentations were analyzed. The key quality indicators of diesel fuel, such as the cetane number (increased from 48 to 54) and the cold filter plugging point (decreased from 5.8 to 0.2 °C), were significantly improved for the ABE-enriched biodiesel. Thus, the application of biodiesel as the extractant for ABE fermentation would increase ABE production, bypass the energy intensive butanol recovery process, and result in an ABE-enriched biodiesel with improved fuel properties.

Report: Third Regional Seminar, Southeastern Region Adult Basic Education Staff Development Project (New Orleans, May 5-10, 1970).

ERIC Educational Resources Information Center

Southern Regional Education Board, Atlanta, GA.

The regional seminars of the Southern Regional Education Board (SREB) are designed to provide inservice training and planning experiences to foster the professional growth of state department of education, university, and local AE/ABE staff members. The third regional seminar was aimed at developing ongoing, self-supporting training programs…

Test Anxiety Reduction. Student Workbook.

ERIC Educational Resources Information Center

Silverman, Eda; Hanna, Joyce

This student workbook is intended for use in helping teenage and adult basic education (ABE) students reduce their anxiety over tests in general and over the General Educational Development (GED) test in particular. Exercises are provided to help students analyze and understand their feelings about and while taking tests, recall the childhood…

Improving Student Academic Performance through Antibias Education

ERIC Educational Resources Information Center

Harte-Weiner, Philene

2013-01-01

The quantitative study contained herein was conducted to determine if there is a relationship between antibias education (ABE) and student performance on state standardized tests in a South Florida school district. In many districts, raising test scores has become priority with some schools even changing their curriculum so that teachers teach…

Techniques: Integrating the National Issues Forums into ABE Programs.

ERIC Educational Resources Information Center

Colhapp, Barbara Jones

1989-01-01

This article discusses the National Issues Forum (NIF), a national, nonpartisan program designed to encourage citizen discussion of important public issues. The programs try to recreate the town meeting concept of reaching consensus on public issues. Short workbooks are available on two reading levels for issues such as the superpowers, crime,…

Adult Basic Educators' Descriptions of Standards Implementation and Its Influence on Cognitive Rigor

ERIC Educational Resources Information Center

Olson, Lia Conklin

2017-01-01

With only four years of mandated state-standards in adult basic education (ABE), very little is known about the influence of state-standards implementation on the unique learning needs of its adult students. Two decades of research on standards-based education in the U.S. K12 system has yielded widely debated results regarding the impact of…

The AstroBiology Explorer (ABE) MIDEX Mission: Using Infrared Spectroscopy to Identify Organic Molecules in Space

NASA Technical Reports Server (NTRS)

Sandford, S. A.

2002-01-01

The AstroBiology Explorer (ABE) mission is one of four selected for Phase A Concept Study in NASA's current call for MIDEX class missions. ABE is a cooled space telescope equipped with spectrographs covering the 2.5-20 micron spectral range. The ABE mission is devoted to the detection and identification of organic and related molecular species in space. ABE is currently under study at NASA's Ames Research Center in collaboration with Ball Aerospace.

Dissemination Models: Dynamic Ways to Get the Message Out.

ERIC Educational Resources Information Center

Niemi, John A.

In response to the need of Adult Basic Education (ABE) practitioners for means of disseminating information concerning new ideas and practices in their field (particularly the results of "309 Projects" funded under Title III of the Amendments to the Elementary and Secondary Education Act of 1966), The Iowa Model was developed in which the…

Test Bank. NetNews. Volume 8, Number 1, Winter 2008

ERIC Educational Resources Information Center

LDA of Minnesota, 2008

2008-01-01

Minnesota Adult Basic Education (ABE) providers are mandated to use CASAS (Comprehensive Adult Student Assessment System) Reading or Math or TABE (Tests for Adult Basic Education) Reading or Math. This issue of "NetNews" introduces the Test Bank: a variety of informal reading, spelling, and writing assessments available for Minnesota ABE…

Building Integrated Pathways to Sustainable Careers: An Introduction to the Accelerated Opportunity Initiative

ERIC Educational Resources Information Center

Pleasants, Rachel

2011-01-01

"Accelerating Opportunity" responds to the nation's growing need for improved pathways from Adult Basic Education (ABE) to credentials of value in the labor market. It builds on promising practices developed in "Breaking Through," an initiative of Jobs for the Future and the National Council for Workforce Education, and…

Reading Component Skills of Learners in Adult Basic Education

ERIC Educational Resources Information Center

MacArthur, Charles A.; Konold, Timothy R.; Glutting, Joseph J.; Alamprese, Judith A.

2010-01-01

The purposes of this study were to investigate the reliability and construct validity of measures of reading component skills with a sample of adult basic education (ABE) learners, including both native and nonnative English speakers, and to describe the performance of those learners on the measures. Investigation of measures of reading components…

Let ABE Do It. Basic Education in the Workplace.

ERIC Educational Resources Information Center

Mark, Jorie Lester, Ed.

This publication highlights business, industry, union, and Job Training Partnership Act (JTPA)-supported efforts to provide public and private employees, as well as some prospective employees, with the basic literacy skills they need to perform in the workplace. Basic or remedial education users listed in this directory include 198 companies or…

End-of-Year 2010-11 Progress Report to the Legislature: Implementation and Impact of the Workforce Investment Act, Title II Adult Education and Family Literacy Act

ERIC Educational Resources Information Center

CASAS - Comprehensive Adult Student Assessment Systems (NJ1), 2012

2012-01-01

The Federal Workforce Investment Act (WIA), Title II: Adult Education and Family Literacy Act (AEFLA) provides funding for states and territories to provide instruction in English as a Second Language (ESL), Adult Basic Education (ABE), and Adult Secondary Education (ASE) to adults in need of these literacy services. California State Budget Act…

Integrating Research and Extension for the Nsf-Reu Program in Water Resources

NASA Astrophysics Data System (ADS)

Judge, J.; Migliaccio, K.; Gao, B.; Shukla, S.; Ehsani, R.; McLamore, E.

2011-12-01

Providing positive and meaningful research experiences to students in their undergraduate years is critical for motivating them to pursue advanced degrees or research careers in science and engineering. Such experiences not only offer training for the students in problem solving and critical thinking via hands-on projects, but also offer excellent mentoring and recruiting opportunities for the faculty advisors. The goal of the Research Experience for Undergraduates (REU) Program in the Agricultural and Biological Engineering Department (ABE) at the University of Florida (UF) is to provide eight undergraduate students a unique opportunity to conduct research in water resources using interdisciplinary approaches, integrating research and extension. The students are selected from diverse cultural and educational backgrounds. The eight-week REU Program utilizes the extensive infrastructure of UF - Institute of Food and Agricultural Sciences (IFAS) through the Research and Education Centers (RECs). Two students are paired to participate in their own project under the direct supervision of one of the four research mentors. Four of the eight students are located at the main campus, in Gainesville, Fl, and four remaining students are located off-campus, at the RECs, where some of the ABE faculty are located. The students achieve an enriching cohort experience through social networking, daily blogs, and weekly video conferences to share their research and other REU experiences. The students are co-located during the Orientation week and also during the 5-day Florida Waters Tour. Weekly group meetings and guest lectures are conducted via synchronously through video conferencing. The integration of research and extension is naturally achieved through the projects at the RECs, the guest lectures, Extension workshops, and visits to the Water Management Districts in Florida. In the last two years of the Program, we have received over 80 applicants, from four-year and advanced degree offering institutions and a variety of majors such as Geology, Meteorology, Environmental Sciences & Engineering, Civil Engineering, Water Resources, Agricultural Engineering, Physics, Geography, Chemical Engineering, to name a few. This model of providing integrated research and extension opportunities in hydrology where not all the REU participants are physically co-located, is unique and can be extended to other disciplines.

Adult Basic Education: A Guide to Upgrading in British Columbia's "Public Post-Secondary Institutions." An Articulation Handbook, 2009-2010 Edition

ERIC Educational Resources Information Center

Ministry of Advanced Education and Labour Market Development, 2009

2009-01-01

This is the twenty-fourth edition of the Adult Basic Education (ABE) Articulation Handbook for British Columbia's public post-secondary institutions. It has been updated for 2009-2010 through the dedicated efforts of the educators who participate in the working and steering committees. Articulation is a dynamic process that will never be…

Rediscovery and Exploration of Magic Mountain, Explorer Ridge, NE Pacific

NASA Astrophysics Data System (ADS)

Embley, R. W.

2002-12-01

A two-part exploration program at Explorer Ridge, the northernmost spreading segment of the NE Pacific spreading centers, was conducted in two phases during June to August of 2002. A robust hydrothermal system (Magic Mountain) was found in this area in the early 1980s by the Canadian PISCES IV submersible, but its dimensions and geologic relationships were not well determined due to limited dives and poor navigation. The first part of the 2002 exploration program utilized an EM300 multibeam sonar on T. G. Thompson, the autonomous vehicle ABE, and a CTD/rosette system to map the seafloor and conduct hydrothermal plume surveys. While ABE conducted detailed surveys in the area where the most intense hydrothermal plume was found on the initial CTD survey, the T. G. Thompson conducted additional multibeam surveys, CTD casts and CTD tow-yos on the other second order segments up to 60 km away. This increased the efficiency of the expedition by at least 30%. After 12 days on site, a multibeam map was completed of the entire segment, the spatial distribution and character of the hydrothermal plumes were mapped out and a section of seafloor measuring 2 x 5.5 km was mapped in detail with ABE. The ABE used two sonar systems, a previously proven Imagenex pencil beam sonar, and, for the first time, a multibeam sonar (SM2000). In addition to the high-resolution bathymetry (1 m grid-cell size resolution for the SM2000), ABE collected temperature, optical backscatter, eH redox potential, and magnetic field data. Using the CTD and ABE data, a major hydrothermal system was easily located on the seafloor during the second part of the exploration program using the ROPOS remotely operated vehicle. The Magic Mountain hydrothermal system is located almost entirely on the eastern constructional shoulder of the ridge eastward of the rim of the eastern boundary fault of the axial valley. This is in contrast to most other hydrothermal systems on intermediate rate spreading ridges, which are either centered within the neovolcanic zone or associated with a boundary fault. The active venting occurs over at least 400 m along axis and is mostly concentrated in clusters of high temperature chimneys, each about 50 m in diameter. Two of these clusters have a basal sulfide mound. There is obvious structural control of many of the vents - many lie along or in line with distinct fissures or small faults and the entire field appears to have developed within a shallow graben formed on the ridge flank. Most of the chimneys consist of relatively friable sulfates (barite/anhydrite) that vent clear fluid at up to about 290°C. Several larger active chimneys consist primarily of sulfide minerals that emit gray smoke with temperatures as high as 312§ C. Biologic communities were primarily associated with the more stable sulfide structures. The mixture of proven technology used from a capable surface vessel during the 2002 Explorer Ridge program, including a cutting edge deep AUV and a large ROV, provided the tools to explore a little-known site at a full range of scales in a short amount of time and collect invaluable samples for research. These initial data sets from the 2002 exploration program set the stage for more detailed studies of this unique hydrothermal system in the future.

Focus on Basics: Connecting Research and Practice: Workplace Education, Volume 7, Issue B

ERIC Educational Resources Information Center

National Center for the Study of Adult Learning and Literacy, 2004

2004-01-01

This issue focuses on workplace education and includes the following articles: "After the Grant Is Over" (Connie Nelson); "Around to Stay" (Shirley Penn and Mary Zorn); "A Conversation with FOB: What Benefits Does Workplace Ed Offer the Provider?"; "Reading Work" (Tracy Defoe and Sue Folinsbee); "Much More than ABE" (Don Block and Lori…

Subgroups of Adult Basic Education Learners with Different Profiles of Reading Skills

ERIC Educational Resources Information Center

MacArthur, Charles A.; Konold, Timothy R.; Glutting, Joseph J.; Alamprese, Judith A.

2012-01-01

The purpose of this study was to identify subgroups of adult basic education (ABE) learners with different profiles of skills in the core reading components of decoding, word recognition, spelling, fluency, and comprehension. The analysis uses factor scores of those 5 reading components from on a prior investigation of the reliability and…

Increasing Access to Learning for the Adult Basic Education Learner with Learning Disabilities: Evidence-Based Accommodation Research

ERIC Educational Resources Information Center

Gregg, Noel

2012-01-01

Accommodating adult basic education (ABE) learners with learning disabilities (LD) is common practice across many instructional, testing, and work settings. However, the results from this literature search indicate that very few empirically based studies are available to support or reject the effectiveness of a great deal of accommodation…

Mobilizing Adult Basic Education. Final Project Report.

ERIC Educational Resources Information Center

Fausto, Vincent J.

An adult basic education (ABE) project was undertaken in 1977 in southern New Jersey to take instruction to students in rural areas. The region was one in which some 75% of the population had not completed high school. Centered in Millville with satellite centers in four rural townships whose secondary students are bussed to Millville, the project…

Technical and economic assessment of processes for the production of butanol and acetone. Phase two: analysis of research advances. Energy Conversion and Utilization Technologies Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1984-08-01

The initial objective of this work was to develop a methodology for analyzing the impact of technological advances as a tool to help establish priorities for R and D options in the field of biocatalysis. As an example of a biocatalyzed process, butanol/acetone fermentation (ABE process) was selected as the specific topic of study. A base case model characterizing the technology and economics associated with the ABE process was developed in the previous first phase of study. The project objectives were broadened in this second phase of work to provide parametric estimates of the economic and energy impacts of amore » variety of research advances in the hydrolysis, fermentation and purification sections of the process. The research advances analyzed in this study were based on a comprehensive literature review. The six process options analyzed were: continuous ABE fermentaton; vacuum ABE fermentation; Baelene solvent extraction; HRI's Lignol process; improved prehydrolysis/dual enzyme hydrolysis; and improved microorganism tolerance to butanol toxicity. Of the six options analyzed, only improved microorganism tolerance to butanol toxicity had a significant positive effect on energy efficiency and economics. This particular process option reduced the base case production cost (including 10% DCF return) by 20% and energy consumption by 16%. Figures and tables.« less

End-of-Year 2009-10 Progress Report to the California Legislature: Implementation and Impact of the Workforce Investment Act (WIA), Title II Adult Education and Family Literacy Act

ERIC Educational Resources Information Center

CASAS - Comprehensive Adult Student Assessment Systems (NJ1), 2011

2011-01-01

The Federal Workforce Investment Act (WIA), Title II: Adult Education and Family Literacy Act (AEFLA) provide funding for states and territories to provide instruction in English as a Second Language (ESL), Adult Basic Education (ABE), and Adult Secondary Education (ASE) to adults in need of these literacy services. California State Budget Act…

Production of Butanol (A Biofuel) from Agricultural Residues: Part I - Use of Barley Straw Hydrolysate

USDA-ARS?s Scientific Manuscript database

Fermentation of dilute sulfuric acid barley straw hydrolyzate (BSH; undiluted/untreated) by Clostridium beijerinckii P260 resulted in the production of 7.09 gL**-1 ABE (acetone butanol ethanol; AB or ABE), an ABE yield of 0.33, and productivity of 0.10 gL**-1h**-1. This level of ABE is much less th...

"My Turn," Women's Goals and Motivations in a Diploma Program: A Constructive-Developmental Approach

ERIC Educational Resources Information Center

Drago-Severson, Eleanor

2014-01-01

This research was part of a larger, mixed-methods study, funded by the National Center for the Study of Adult Learning and Literacy, which examined the learning and change experiences of 41 learners in three ABE/ESOL programs. This paper examines a Polaroid diploma program with a focus on women workers, employing gender (relational) and…

Enhancement of lipase catalyzed-fatty acid methyl esters production from waste activated bleaching earth by nullification of lipase inhibitors.

PubMed

Dwiarti, Lies; Ali, Ehsan; Park, Enoch Y

2010-01-01

This study sought to identify inhibitory factors of lipase catalyzed-fatty acid methyl esters (FAME) production from waste activated bleaching earth (wABE). During the vegetable oil refinery process, activated bleaching earth (ABE) is used for removing the impure compounds, but adsorbs vegetable oil up to 35-40% as on a weight basis, and then the wABE is discarded as waste material. The impurities were extracted from the wABE with methanol and evaluated by infra-red (IR) spectroscopy, which revealed that some were chlorophyll-plant pigments. The chlorophylls inhibited the lipase during FAME conversion from wABE. The inhibition by a mixture of chlorophyll a and b was found to be competitive. The inhibition of the enzymatic hydrolysis of waste vegetable oil contained in wABE by chlorophyll a alone was competitive, while the inhibition by chlorophyll b alone was non-competitive. Furthermore, the addition of a small amount of alkali nullified this inhibitory effect and accelerated the FAME production rate. When 0.9% KOH (w/w wABE) was added to the transesterification reaction with only 0.05% lipase (w/w wABE), the maximum FAME production rate improved 120-fold, as compared to that without the addition of KOH. The alkali-combined lipase significantly enhanced the FAME production rate from wABE, in spite of the presence of the plant pigments, and even when a lower amount of lipase was used as a catalyst.

ABE/Pre-Vocational Skills Program. A Teacher's Guide and Curriculum for Pre-Clerical Training.

ERIC Educational Resources Information Center

Johnson, Brenda J.

This manual is designed for teacher use for a 10-week course to prepare chronically unemployed adults to enter traditional clerical courses or job training programs offered by temporary-employment-agencies. The competency-based curriculum provides adults with basic skills in grammar, usage, reading, writing, listening, mathematics, keyboarding,…

Efficient production of acetone-butanol-ethanol (ABE) from cassava by a fermentation-pervaporation coupled process.

PubMed

Li, Jing; Chen, Xiangrong; Qi, Benkun; Luo, Jianquan; Zhang, Yuming; Su, Yi; Wan, Yinhua

2014-10-01

Production of acetone-butanol-ethanol (ABE) from cassava was investigated with a fermentation-pervaporation (PV) coupled process. ABE products were in situ removed from fermentation broth to alleviate the toxicity of solvent to the Clostridium acetobutylicum DP217. Compared to the batch fermentation without PV, glucose consumption rate and solvent productivity increased by 15% and 21%, respectively, in batch fermentation-PV coupled process, while in continuous fermentation-PV coupled process running for 304 h, the substrate consumption rate, solvent productivity and yield increased by 58%, 81% and 15%, reaching 2.02 g/Lh, 0.76 g/Lh and 0.38 g/g, respectively. Silicalite-1 filled polydimethylsiloxane (PDMS)/polyacrylonitrile (PAN) membrane modules ensured media recycle without significant fouling, steadily generating a highly concentrated ABE solution containing 201.8 g/L ABE with 122.4 g/L butanol. After phase separation, a final product containing 574.3g/L ABE with 501.1g/L butanol was obtained. Therefore, the fermentation-PV coupled process has the potential to decrease the cost in ABE production. Copyright © 2014 Elsevier Ltd. All rights reserved.

Effects of Averrhoa bilimbi leaf extract on blood glucose and lipids in streptozotocin-diabetic rats.

PubMed

Pushparaj, P; Tan, C H; Tan, B K

2000-09-01

The present study was designed to investigate the hypoglycemic and hypolipidemic activities of an ethanolic extract of Averrhoa bilimbi Linn. leaves (Oxalidaceae, Common name: Bilimbi) in streptozotocin (STZ)-diabetic rats. The optimal hypoglycemic dose (125 mg kg(-1)) was determined by performing the oral glucose tolerance test (OGTT) in both normal and STZ-diabetic rats. To investigate the effect of repeated administration of an ethanolic extract of Averrhoa bilimbi (ABe) leaves, diabetic rats were treated with vehicle (distilled water), ABe (125 mg kg(-1)) or metformin (500 mg kg(-1)) twice a day for 2 weeks. Like metformin, ABe significantly lowered blood glucose by 50% and blood triglyceride by 130% when compared with the vehicle. ABe also significantly increased the HDL-cholesterol concentrations by 60% compared with the vehicle. ABe thus significantly increased the anti-atherogenic index and HDL-cholesterol/total cholesterol ratio. However, like metformin, ABe did not affect total cholesterol and LDL-cholesterol concentrations, but significantly reduced the kidney lipid peroxidation level. These data show that ABe has hypoglycemic, hypotriglyceridemic, anti-lipid peroxidative and anti-atherogenic properties in STZ-diabetic rats.

Competency Mapping: A New Concept in ABE Needs Assessment.

ERIC Educational Resources Information Center

Turman, Carlene L.

The concept of "competency mapping" emerged from a study of the state-of-the-art of adult education needs assessment to provide the basis for development of a model for states to adopt or adapt to fulfill requirements of the 1978 amendments to the Adult Education Act. Need was defined as a lack of functional competency and those problems…

How Is Education Perceived on the Inside?: A Preliminary Study of Adult Males in a Correctional Setting

ERIC Educational Resources Information Center

Moeller, Michelle; Day, Scott L.; Rivera, Beverly D.

2004-01-01

This study explores a group of inmates' perceptions of their correctional education and environment based on Fetterman's 1994 idea of empowerment evaluation. A group of 16 male inmates were randomly selected from GED and ABE courses in a high minimum correctional facility in Illinois. A self-administered questionnaire included 5 topics:…

A Quantile Regression Approach to Understanding the Relations among Morphological Awareness, Vocabulary, and Reading Comprehension in Adult Basic Education Students

ERIC Educational Resources Information Center

Tighe, Elizabeth L.; Schatschneider, Christopher

2016-01-01

The purpose of this study was to investigate the joint and unique contributions of morphological awareness and vocabulary knowledge at five reading comprehension levels in adult basic education (ABE) students. We introduce the statistical technique of multiple quantile regression, which enabled us to assess the predictive utility of morphological…

BIOASPEN: System for technology development

NASA Technical Reports Server (NTRS)

1986-01-01

The public version of ASPEN was installed in the VAX 11/750 computer. To examine the idea of BIOASPEN, a test example (the manufacture of acetone, butanol, and ethanol through a biological route) was chosen for simulation. Previous reports on the BIOASPEN project revealed the limitations of ASPEN in modeling this process. To overcome some of the difficulties, modules were written for the acid and enzyme hydrolyzers, the fermentor, and a sterilizer. Information required for these modules was obtained from the literature whenever possible. Additional support modules necessary for interfacing with ASPEN were also written. Some of ASPEN subroutines were themselves altered in order to ensure the correct running of the simulation program. After testing of these additions and charges was completed, the Acetone-Butanol-Ethanol (ABE) process was simulated. A release of ASPEN (which contained the Economic Subsystem) was obtained and installed. This subsection was tested and numerous charges were made in the FORTRAN code. Capital investment and operating cost studies were performed on the ABE process. Some alternatives in certain steps of the ABE simulation were investigated in order to elucidate their effects on the overall economics of the process.

Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing.

PubMed

Liu, Zhen; Lu, Zongyang; Yang, Guang; Huang, Shisheng; Li, Guanglei; Feng, Songjie; Liu, Yajing; Li, Jianan; Yu, Wenxia; Zhang, Yu; Chen, Jia; Sun, Qiang; Huang, Xingxu

2018-06-14

A recently developed adenine base editor (ABE) efficiently converts A to G and is potentially useful for clinical applications. However, its precision and efficiency in vivo remains to be addressed. Here we achieve A-to-G conversion in vivo at frequencies up to 100% by microinjection of ABE mRNA together with sgRNAs. We then generate mouse models harboring clinically relevant mutations at Ar and Hoxd13, which recapitulates respective clinical defects. Furthermore, we achieve both C-to-T and A-to-G base editing by using a combination of ABE and SaBE3, thus creating mouse model harboring multiple mutations. We also demonstrate the specificity of ABE by deep sequencing and whole-genome sequencing (WGS). Taken together, ABE is highly efficient and precise in vivo, making it feasible to model and potentially cure relevant genetic diseases.

Kinetic Study of Acetone-Butanol-Ethanol Fermentation in Continuous Culture

PubMed Central

Buehler, Edward A.; Mesbah, Ali

2016-01-01

Acetone-butanol-ethanol (ABE) fermentation by clostridia has shown promise for industrial-scale production of biobutanol. However, the continuous ABE fermentation suffers from low product yield, titer, and productivity. Systems analysis of the continuous ABE fermentation will offer insights into its metabolic pathway as well as into optimal fermentation design and operation. For the ABE fermentation in continuous Clostridium acetobutylicum culture, this paper presents a kinetic model that includes the effects of key metabolic intermediates and enzymes as well as culture pH, product inhibition, and glucose inhibition. The kinetic model is used for elucidating the behavior of the ABE fermentation under the conditions that are most relevant to continuous cultures. To this end, dynamic sensitivity analysis is performed to systematically investigate the effects of culture conditions, reaction kinetics, and enzymes on the dynamics of the ABE production pathway. The analysis provides guidance for future metabolic engineering and fermentation optimization studies. PMID:27486663

Integrated in situ gas stripping-salting-out process for high-titer acetone-butanol-ethanol production from sweet sorghum bagasse.

PubMed

Wen, Hao; Chen, Huidong; Cai, Di; Gong, Peiwen; Zhang, Tao; Wu, Zhichao; Gao, Heting; Li, Zhuangzhuang; Qin, Peiyong; Tan, Tianwei

2018-01-01

The production of biobutanol from renewable biomass resources is attractive. The energy-intensive separation process and low-titer solvents production are the key constraints on the economy-feasible acetone-butanol-ethanol (ABE) production by fermentation. To decrease energy consumption and increase the solvents concentration, a novel two-stage gas stripping-salting-out system was established for effective ABE separation from the fermentation broth using sweet sorghum bagasse as feedstock. The ABE condensate (143.6 g/L) after gas stripping, the first-stage separation, was recovered and introduced to salting-out process as the second-stage. K 4 P 2 O 7 and K 2 HPO 4 were used, respectively. The effect of saturated salt solution temperature on final ABE concentration was also investigated. The results showed high ABE recovery (99.32%) and ABE concentration (747.58 g/L) when adding saturated K 4 P 2 O 7 solution at 323.15 K and 3.0 of salting-out factor. On this condition, the energy requirement of the downstream distillation process was 3.72 MJ/kg of ABE. High-titer cellulosic ABE production was separated from the fermentation broth by the novel two-stage gas stripping-salting-out process. The process was effective, which reduced the downstream process energy requirement significantly.

Butanol production from food waste: a novel process for producing sustainable energy and reducing environmental pollution.

PubMed

Huang, Haibo; Singh, Vijay; Qureshi, Nasib

2015-01-01

Waste is currently a major problem in the world, both in the developing and the developed countries. Efficient utilization of food waste for fuel and chemical production can positively influence both the energy and environmental sustainability. This study investigated using food waste to produce acetone, butanol, and ethanol (ABE) by Clostridium beijerinckii P260. In control fermentation, 40.5 g/L of glucose (initial glucose 56.7 g/L) was used to produce 14.2 g/L of ABE with a fermentation productivity and a yield of 0.22 g/L/h and 0.35 g/g, respectively. In a similar fermentation 81 g/L of food waste (containing equivalent glucose of 60.1 g/L) was used as substrate, and the culture produced 18.9 g/L ABE with a high ABE productivity of 0.46 g/L/h and a yield of 0.38 g/g. Fermentation of food waste at higher concentrations (129, 181 and 228 g/L) did not remarkably increase ABE production but resulted in high residual glucose due to the culture butanol inhibition. An integrated vacuum stripping system was designed and applied to recover butanol from the fermentation broth simultaneously to relieve the culture butanol inhibition, thereby allowing the fermentation of food waste at high concentrations. ABE fermentation integrated with vacuum stripping successfully recovered the ABE from the fermentation broth and controlled the ABE concentrations below 10 g/L during fermentation when 129 g/L food waste was used. The ABE productivity with vacuum fermentation was 0.49 g/L/h, which was 109 % higher than the control fermentation (glucose based). More importantly, ABE vacuum recovery and fermentation allowed near-complete utilization of the sugars (~98 %) in the broth. In these studies it was demonstrated that food waste is a superior feedstock for producing butanol using Clostridium beijerinckii. Compared to costly glucose, ABE fermentation of food waste has several advantages including lower feedstock cost, higher productivity, and less residual sugars.

Understanding Instructions.

ERIC Educational Resources Information Center

Milburn, Val

This guide is intended to help adult basic education (ABE) teachers teach their students to understand instructions in their daily lives. The 25 learning activities included all develop students' skills in the area of following directions by using basic situations drawn from everyday life. The following activities are included: sequencing pictures…

ABE NetNews, Volume 2.

ERIC Educational Resources Information Center

ABE NetNews, 2002

2002-01-01

These four newsletters focus on issues related to adult basic education and the challenges faced when teaching adult learners. The first issue introduces the LDA (Learning Disabilities Association) Learning Center, a private, nonprofit agency intended to maximize the potential of all people with learning disabilities or related learning…

Characterization of ultra-fine grained aluminum produced by accumulative back extrusion (ABE)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Alihosseini, H., E-mail: hamid.alihossieni@gmail.com; Materials Science and Engineering Department, Engineering School, Amirkabir University, Tehran; Faraji, G.

2012-06-15

In the present work, the microstructural evolutions and microhardness of AA1050 subjected to one, two and three passes of accumulative back extrusion (ABE) were investigated. The microstructural evolutions were characterized using transmission electron microscopy. The results revealed that applying three passes of accumulative back extrusion led to significant grain refinement. The initial grain size of 47 {mu}m was refined to the grains of 500 nm after three passes of ABE. Increasing the number of passes resulted in more decrease in grain size, better microstructure homogeneity and increase in the microhardness. The cross-section of ABEed specimen consisted of two different zones:more » (i) shear deformation zone, and (ii) normal deformation zone. The microhardness measurements indicated that the hardness increased from the initial value of 31 Hv to 67 Hv, verifying the significant microstructural refinement via accumulative back extrusion. - Highlights: Black-Right-Pointing-Pointer A significant grain refinement can be achieved in AA1050, Al alloy by applying ABE. Black-Right-Pointing-Pointer Microstructural homogeneity of ABEed samples increased by increasing the number of ABE cycles. Black-Right-Pointing-Pointer A substantial increase in the hardness, from 31 Hv to 67 Hv, was recorded.« less

Modeling the Relations Among Morphological Awareness Dimensions, Vocabulary Knowledge, and Reading Comprehension in Adult Basic Education Students

PubMed Central

Tighe, Elizabeth L.; Schatschneider, Christopher

2016-01-01

This study extended the findings of Tighe and Schatschneider (2015) by investigating the predictive utility of separate dimensions of morphological awareness as well as vocabulary knowledge to reading comprehension in adult basic education (ABE) students. We competed two- and three-factor structural equation models of reading comprehension. A three-factor model of real word morphological awareness, pseudoword morphological awareness, and vocabulary knowledge emerged as the best fit and accounted for 79% of the reading comprehension variance. The results indicated that the constructs contributed jointly to reading comprehension; however, vocabulary knowledge was the only potentially unique predictor (p = 0.052), accounting for an additional 5.6% of the variance. This study demonstrates the feasibility of applying a latent variable modeling approach to examine individual differences in the reading comprehension skills of ABE students. Further, this study replicates the findings of Tighe and Schatschneider (2015) on the importance of differentiating among dimensions of morphological awareness in this population. PMID:26869981

Modeling the Relations Among Morphological Awareness Dimensions, Vocabulary Knowledge, and Reading Comprehension in Adult Basic Education Students.

PubMed

Tighe, Elizabeth L; Schatschneider, Christopher

2016-01-01

This study extended the findings of Tighe and Schatschneider (2015) by investigating the predictive utility of separate dimensions of morphological awareness as well as vocabulary knowledge to reading comprehension in adult basic education (ABE) students. We competed two- and three-factor structural equation models of reading comprehension. A three-factor model of real word morphological awareness, pseudoword morphological awareness, and vocabulary knowledge emerged as the best fit and accounted for 79% of the reading comprehension variance. The results indicated that the constructs contributed jointly to reading comprehension; however, vocabulary knowledge was the only potentially unique predictor (p = 0.052), accounting for an additional 5.6% of the variance. This study demonstrates the feasibility of applying a latent variable modeling approach to examine individual differences in the reading comprehension skills of ABE students. Further, this study replicates the findings of Tighe and Schatschneider (2015) on the importance of differentiating among dimensions of morphological awareness in this population.

Cellulosic Substrates and Challenges Ahead

USDA-ARS?s Scientific Manuscript database

The cost of production of butanol (acetone-butanol-ethanol; or ABE) is determined by feedstock prices, fermentation, recovery, by-product credits and the waste water treatment. Along these lines, we have an intensive research program on the use of various agricultural substrates, fermentation strate...

Reaching ABE Students: Lessons from the Iowa Studies.

ERIC Educational Resources Information Center

Beder, Hal

1990-01-01

An Iowa survey of 351 adult basic education participants uncovered 10 motivations for participation, which were used to develop a cluster-analysis market segmentation. Another Iowa study found 4 basic causes for nonparticipation: low perception of need, perceived difficulty, dislike of school, and situational barriers. (SK)

Adult Academy Tutor Training Manual.

ERIC Educational Resources Information Center

Isserlis, Janet; And Others

This handbook is for volunteer tutors, student interns, and VISTA volunteers working with adult basic education (ABE) and English-as-a-Second-Language (ESL) learners. The community-based handbook contains information about adult literacy and tutoring--what tutors do, who the learners are, and how the literacy learning process works. Introductory…

Transferring Learning from the Workshop to the Classroom

ERIC Educational Resources Information Center

Johnson, Kimberly A.

2009-01-01

As coordinator of the ABE Teaching and Learning Advancement System (ATLAS) based in the Hamline University School of Education in St. Paul, the author does many workshops or conference sessions in Minnesota's nine professional development regions each year. Typical single-day events offer multiple 90-minute workshops. She often questions the…

Preparation for Instruction. TECHNIQUES.

ERIC Educational Resources Information Center

Jones, Edward V.

1985-01-01

An "orientation" checklist is proposed to aid the adult basic education (ABE) teacher in preparing for instruction. The list reflects a number of issues teachers should consider carefully before meeting with students. The issues concern (1) information about the learners and their characteristics and needs that can be taken into account in…

ABE NetNews, Volume 1.

ERIC Educational Resources Information Center

ABE NetNews, 2001

2001-01-01

These four newsletters focus on issues related to adult basic education and the challenges faced when teaching adult learners. The first issue looks at the characteristics of learning disabilities, providing information to help teachers determine whether a learner has a learning disability and who might be appropriately referred for testing. The…

An Annotated Bibliography of Adult ESL Instructional Materials.

ERIC Educational Resources Information Center

Escobar, Joanna Sculley; Daugherty, John

This annotated bibliography of adult English as a second language (ESL) student materials, available at the Illinois ESL/Adult Basic Education (ABE) Services Center, was prepared for adult ESL administrators, coordinators, teachers, and teacher aides to provide a representative sampling of materials available and to assist in the selection of…

Teach Your Children: Learning Differences. Final Report.

ERIC Educational Resources Information Center

Fisher, Allison L.; Willard, Penny

A three-part series of evening workshops was designed to help adult basic education (ABE) parents identify learning styles and develop communication and advocacy skills, assertiveness, and self-esteem. At the workshops, instructors from the center presented an adaptation of curriculum on self-esteem for parenting developed by the Center for…

Investigating Predictors of Spelling Ability for Adults with Low Literacy Skills

ERIC Educational Resources Information Center

Talwar, Amani; Cote, Nicole Gilbert; Binder, Katherine

2014-01-01

This study examined whether the spelling abilities of adults with low literacy skills could be predicted by their phonological, orthographic, and morphological awareness. Sixty Adult Basic Education (ABE) students completed several literacy tasks. It was predicted that scores on phonological and orthographic tasks would explain variance in…

Identifying Organic Molecules in Space: The AstroBiology Explorer (ABE) Mission Concept

NASA Technical Reports Server (NTRS)

Ennico, Kimberly; Sandford, S.; Allamandola, L.; Bregman, J.; Cohen, M.; Cruikshank, D.; Dumas, C.; Greene, T.; Hudgins, D.; Kwok, S.

2004-01-01

The AstroBiology Explorer (ABE) mission concept consists of a modest dedicated space observatory having a 60 cm class primary mirror cooled to T less than 50 K equipped with medium resolution cross-dispersed spectrometers having cooled large format near- and mid-infrared detector arrays. Such a system would be capable of addressing outstanding problems in Astrochemistry and Astrophysics that are particularly relevant to Astrobiology and addressable via astronomical observation. The mission's observaticxiai program woiild make fundamental scieztific: prngress in establishing the nature, distribution, formation and evolution of organic and other molecular materials in the following extra-terrestrial environments: 1) The Outflow of Dying Stars; 2) The Diffuse Interstellar Medium (DISM); 3) Dense Molecular Clouds, Star Formation Regions, and Young Stellar/Planetary Systems; 4) Planets, Satellites, and Small Bodies within the Solar System; and 5) The Interstellar Media of Other Galaxies ABE could make fundamental progress in all of these area by conducting a 1 to 2 year mission to obtain a coordinated set of infrared spectroscopic observations over the 2.5 - 20 micron spectral range at a spectral resolution of R greater than 2500 of about 1500 galaxies, stars, planetary nebulae, young stellar objects, and solar system objects.

Determining Prevalence of Acute Bilirubin Encephalopathy in Developing Countries

ClinicalTrials.gov

2015-11-11

Demonstrate BIND II Score of >=5, is Valid for Detecting Moderate to Severe ABE in Neonates <14 Days Old.; Demonstrate Community-BIND Instrument, a Modified BIND II, is a Valid and Reliable Tool for Detecting ABE.; Demonstrate That Community-BIND Can be Used for Acquiring Population-based Prevalence of ABE in the Community.

Effect of cellulosic sugar degradation products (furfural and hydroxymethylfurfural) on acetone-butanol-ethanol (ABE) fermentation using Clostridium beijerinckii P260

USDA-ARS?s Scientific Manuscript database

Studies were performed to identify chemicals present in wheat straw hydrolysate (WSH) that enhance acetone butanol ethanol (ABE) productivity. These chemicals were identified as furfural and hydroxymethyl furfural (HMF). Control experiment resulted in the production of 21.09-21.66 gL**-1 ABE with a ...

Acetone-butanol-ethanol production from substandard and surplus dates by Egyptian native Clostridium strains.

PubMed

Abd-Alla, Mohamed Hemida; Zohri, Abdel-Naser Ahmed; El-Enany, Abdel-Wahab Elsadek; Ali, Shimaa Mohamed

2015-04-01

One hundred and seven mesophilic isolates of Clostridium were isolated from agricultural soils cultivated with different plants in Assuit Governorate, Egypt. Eighty isolates (out of 107) showed the ability to produce ABE (Acetone, butanol and ethanol) on T6 medium ranging from 0.036 to 31.89 g/L. The highest numbers of ABE producing isolates were obtained from soil samples of potato contributing 27 isolates, followed by 18 isolates from wheat and 10 isolates from onion. On the other hand, there were three native isolates that produced ABE more than those produced by the reference isolate Clostridium acetobutylicum ATCC 824 (11.543 g/L). The three isolates were identified based on phenotypic and gene encoding 16S rRNA as Clostridium beijerinckii ASU10 (KF372577), Clostridium chauvoei ASU55 (KF372580) and Clostridium roseum ASU58 (KF372581). The highest ABE level from substandard and surplus dates was produced by C. beijerinckii ASU10 (24.07 g/L) comprising butanol 67.15% (16.16 g/L), acetone 30.73% (7.4 g/L) and ethanol 2.12% (0.51 g/L), while C. roseum ASU58 and C. chauvoei ASU55 produced ABE contributing 20.20 and 13.79 g/L, respectively. ABE production by C. acetobutylicum ATCC 824 was 15.01 g/L. This study proved that the native strains C. beijerinckii ASU10 and C. roseum ASU58 have high competitive efficacy on ABE production from economical substrate as substandard and surplus date fruits. Additionally, using this substrate without any nutritional components is considered to be a commercial substrate for desired ABE production. Copyright © 2014 Elsevier Ltd. All rights reserved.

Acetone-butanol-ethanol from sweet sorghum juice by an immobilized fermentation-gas stripping integration process.

PubMed

Cai, Di; Wang, Yong; Chen, Changjing; Qin, Peiyong; Miao, Qi; Zhang, Changwei; Li, Ping; Tan, Tianwei

2016-07-01

In this study, sweet sorghum juice (SSJ) was used as the substrate in a simplified ABE fermentation-gas stripping integration process without nutrients supplementation. The sweet sorghum bagasse (SSB) after squeezing the fermentable juice was used as the immobilized carrier. The results indicated that the productivity of ABE fermentation process was improved by gas stripping integration. A total 24g/L of ABE solvents was obtained from 59.6g/L of initial sugar after 80h of fermentation with gas stripping. Then, long-term of fed-batch fermentation with continuous gas stripping was further performed. 112.9g/L of butanol, 44.1g/L of acetone, 9.5g/L of ethanol (total 166.5g/L of ABE) was produced in overall 312h of fermentation. At the same time, concentrated ABE product was obtained in the condensate of gas stripping. Copyright © 2016 Elsevier Ltd. All rights reserved.

Adult Literacy Learning and Computer Technology: Features of Effective Computer-Assisted Learning Systems.

ERIC Educational Resources Information Center

Fahy, Patrick J.

Computer-assisted learning (CAL) can be used for adults functioning at any academic or grade level. In adult basic education (ABE), CAL can promote greater learning effectiveness and faster progress, concurrent learning and experience with computer literacy skills, privacy, and motivation. Adults who face barriers (financial, geographic, personal,…

Minnesota STAR Project: Meeting the Needs of Struggling Adult Readers

ERIC Educational Resources Information Center

Johnson, Kimberly A.; Frank, Margaret M.

2012-01-01

This paper reports on findings and implications from a two-year evaluation of the Minnesota STudent Achievement in Reading (STAR) Project. This long-term, job-embedded, professional development activity is provided for Minnesota Adult Basic Education (ABE) practitioners serving intermediate-level adult students reading between 4.0 to 8.9 grade…

Biobutanol production from brewer's spent grain hydrolysates by Clostridium beijerinckii.

PubMed

Plaza, Pedro E; Gallego-Morales, Luis Javier; Peñuela-Vásquez, Mariana; Lucas, Susana; García-Cubero, M Teresa; Coca, Mónica

2017-11-01

Brewer's spent grain (BSG) is a promising feedstock for ABE fermentation. Sulfuric acid pretreatment of BSG at pH 1, 121°C and different solid loadings (5-15% w/w) was investigated. Enzymatic hydrolysis and ABE fermentation by Clostridium beijerinckii DSM 6422 of non-washed and washed pretreated BSG were performed to compare monosaccharide release and butanol production. Pretreatment at 15% w/w BSG resulted in higher availability of sugars in both the enzymatic hydrolysates and pretreatment liquid, and overall yields of 75gbutanol/kg BSG and 95gABE/kg BSG were obtained. When the enzymatic hydrolysate from the washed pretreated BSG was fermented, butanol (6.0±0.5g/L) and ABE (7.4±1.0g/L) concentrations were lower compared with 7.5±0.6g/L butanol and 10.0±0.8g/L ABE from a control. The fermentation of the liquid released in the pretreatment at 15% w/w resulted in a butanol production of 6.6±0.8g/L with a total ABE of 8.6±1.3g/L after overliming. Copyright © 2017 Elsevier Ltd. All rights reserved.

Assertion Mechanisms in Programming Languages.

DTIC Science & Technology

1979-11-01

the Construction and Verific3tion of ALPHAPD Programs ", IEEE Transactions on Software Engineering , voL. 2, no. 4, p. 253-265, 1 1-7t [Zelkooitz a...be true at a point in program execut ion. The languaje designer has several options when considering the semantics of an assertion mechanism... Software Engineering , vol. SE-i, no. 2, p. 156-173, June 1975. [Hansen] G. J. Hansen, G. A. Shoults and J. D. Coinmeat, "Construction of a Transportaole

Expanding Learning and Teaching Processes in an ESL/Civics ABE Classroom Using an Interactive Video Lesson Plan in the U.S. Southwest: An Action Research Study

ERIC Educational Resources Information Center

Cajar-Bravo, Aristides

2010-01-01

This study is an action research project that analyzed the ways in which ESL students improve their language learning processes by using as a teaching tool a media literacy video and Civics Education for social skills; it was presented to two groups of 12 students who were attending an ESL/Civics Education Intermediate-Advanced class in an ABE…

Abe Silverstein Leads Tour of the 10- by 10-Foot Supersonic Wind Tunnel

NASA Image and Video Library

1955-11-21

Abe Silverstein, Associate Director of the National Advisory Committee for Aeronautics (NACA) Lewis Flight Propulsion Laboratory, provides a personal tour of the new 10- by 10-Foot Supersonic Wind Tunnel for US Senator George Bender (hat in hand) and General Lemuel Shepherd. Shepherd was Commandant of the Marine Corps and had served in World War I, World War II, and the Korean War. The general was accompanied by Admiral Herbert Leary, in dark uniform. Bender was a Republican Senator from Ohio. Behind Bender is President of the Cleveland Chamber of Commerce Curtis Smith. NACA Lewis managers Eugene Manganiello and Wilson Hunter assist with the tour. Abe Silverstein oversaw all research at the laboratory. Upon taking his post in 1952 he reorganized the research staff and began shifting the focus away from airbreathing aircraft engines to new fields such as high energy fuels, electric propulsion, and nuclear power and propulsion. He was an early advocate of the NACA’s involvement in the space program and crucial to the founding of National Aeronautics and Space Administration in 1958. Silverstein began his career helping design and conduct research in the Full Scale Tunnel in 1929 at the Langley Memorial Aeronautical Laboratory. Silverstein advocated a series of increasingly large supersonic wind tunnels after the war, culminating in the 10- by 10.

Identifying Organic Molecules in Space: The AstroBiology Explorer (ABE) Mission Concept

NASA Technical Reports Server (NTRS)

Ennico, K. A.; Sandford, S. A.; Allamandola, L.; Bregman, J.; Cohen, M.; Cruikshank, D.; Dumas, C.; Greene, T.; Hudgins, D.; Kwok, S.

2004-01-01

The AstroBiology Explorer (ABE) mission concept consists of a dedicated space observatory having a 60 cm class primary mirror cooled to T < 50 K equipped with medium resolution cross-dispersed spectrometers having cooled large format near- and mid-infrared detector arrays. Such a system would be capable of addressing outstanding problems in Astrochemistry and Astrophysics that are particularly relevant to Astrobiology and addressable via astronomical observation. The mission s observational program would make fundamental scientific progress in establishing the nature, distribution, formation and evolution of organic and other molecular materials in the following extra-terrestrial environments: 1) The Outflow of Dying Stars, 2) The Diffuse Interstellar Medium, 3) Dense Molecular Clouds, Star Formation Regions, and Young StellarPlanetary Systems, 4) Planets, Satellites, and Small Bodies within the Solar System, and 5 ) The Interstellar Media of Other Galaxies. ABE could make fundamental progress in all of these areas by conducting a 1 to 2 year mission to obtain a coordinated set of infrared spectroscopic observations over the 2.5-20 micron spectral range at a spectral resolution of R > 2000 of about 1500 objects including galaxies, stars, planetary nebulae, young stellar objects, and solar system objects. Keywords: Astrobiology, infrared, Explorers, interstellar organics, telescope, spectrometer, space, infrared detectors

Evaluation of industrial dairy waste (milk dust powder) for acetone-butanol-ethanol production by solventogenic Clostridium species.

PubMed

Ujor, Victor; Bharathidasan, Ashok Kumar; Cornish, Katrina; Ezeji, Thaddeus Chukwuemeka

2014-01-01

Readily available inexpensive substrate with high product yield is the key to restoring acetone-butanol-ethanol (ABE) fermentation to economic competitiveness. Lactose-replete cheese whey tends to favor the production of butanol over acetone. In the current study, we investigated the fermentability of milk dust powder with high lactose content, for ABE production by Clostridium acetobutylicum and Clostridium beijerinckii. Both microorganisms produced 7.3 and 5.8 g/L of butanol respectively, with total ABE concentrations of 10.3 and 8.2 g/L, respectively. Compared to fermentation with glucose, fermentation of milk dust powder increased butanol to acetone ratio by 16% and 36% for C. acetobutylicum and C. beijerinckii, respectively. While these results demonstrate the fermentability of milk dust powder, the physico-chemical properties of milk dust powder appeared to limit sugar utilization, growth and ABE production. Further work aimed at improving the texture of milk dust powder-based medium would likely improve lactose utilization and ABE production.

Two-stage in situ gas stripping for enhanced butanol fermentation and energy-saving product recovery.

PubMed

Xue, Chuang; Zhao, Jingbo; Liu, Fangfang; Lu, Congcong; Yang, Shang-Tian; Bai, Feng-Wu

2013-05-01

Two-stage gas stripping for butanol recovery from acetone-butanol-ethanol (ABE) fermentation with Clostridium acetobutylicum JB200 in a fibrous bed bioreactor was studied. Compared to fermentation without in situ gas stripping, more ABE (10.0 g/L acetone, 19.2 g/L butanol, 1.7 g/L ethanol vs. 7.9 g/L acetone, 16.2 g/L butanol, 1.4 g/L ethanol) were produced, with a higher butanol yield (0.25 g/g vs. 0.20 g/g) and productivity (0.40 g/L·h vs. 0.30 g/L·h) due to reduced butanol inhibition. The first-stage gas stripping produced a condensate containing 175.6 g/L butanol (227.0 g/L ABE), which after phase separation formed an organic phase containing 612.3g/L butanol (660.7 g/L ABE) and an aqueous phase containing 101.3 g/L butanol (153.2 g/L ABE). After second-stage gas stripping, a highly concentrated product containing 420.3 g/L butanol (532.3 g/L ABE) was obtained. The process is thus effective in producing high-titer butanol that can be purified with much less energy. Copyright © 2012 Elsevier Ltd. All rights reserved.

Two-stage in situ gas stripping for enhanced butanol fermentation and energy-saving product recovery

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xue, C; Zhao, JB; Liu, FF

2013-05-01

Two-stage gas stripping for butanol recovery from acetone-butanol-ethanol (ABE) fermentation with Clostridium acetobutylicum JB200 in a fibrous bed bioreactor was studied. Compared to fermentation without in situ gas stripping, more ABE (10.0 g/L acetone, 19.2 g/L butanol, 1.7 g/L ethanol vs. 7.9 g/L acetone, 16.2 g/L butanol, 1.4 g/L ethanol) were produced, with a higher butanol yield (0.25 g/g vs. 0.20 g/g) and productivity (0.40 g/L.h vs. 0.30 g/L-h) due to reduced butanol inhibition. The first-stage gas stripping produced a condensate containing 175.6 g/L butanol (227.0 g/L ABE), which after phase separation formed an organic phase containing 612.3 g/L butanolmore » (660.7 g/L ABE) and an aqueous phase containing 101.3 g/L butanol (153.2 g/L ABE). After second-stage gas stripping, a highly concentrated product containing 420.3 g/L butanol (532.3 g/L ABE) was obtained. The process is thus effective in producing high-titer butanol that can be purified with much less energy. (C) 2012 Elsevier Ltd. All rights reserved.« less

The Impact of a Professional Development Model on ABE Teachers' Instructional Practice: Teachers Investigating Adult Numeracy

ERIC Educational Resources Information Center

Bingman, Mary Beth; Schmitt, Mary Jane

2008-01-01

The authors present the National Science Foundation project, Teachers Investigating Adult Numeracy (TIAN), a collaborative project of the Center for Literacy Studies at the University of Tennessee and the Technical Education Research Centers, Inc. (TERC) in Cambridge, Massachusetts. The project has developed and tested a model for inservice…

Operation COPE: Family Learning Center Handbook with Mothers Who are Heads of Households.

ERIC Educational Resources Information Center

Davidson, Edmonia W.

The handbook is comprised of materials related to the implementation of Operation COPE, a Washington, D.C., demonstration Adult Basic Education (ABE) project for low-income young mothers who are heads of households, developed by the National Council of Negro Women (NCNW). The project featured a curriculum which integrated coping skills with Adult…

Basic Science Living Skills for Today's World. Teacher's Edition.

ERIC Educational Resources Information Center

Zellers (Robert W.) Educational Services, Johnstown, PA.

This document is a teacher's edition of a basic skills curriculum in science for adult basic education (ABE) students. The course consists of 25 lessons on basic science concepts, designed to give students a good understanding of the biological and physical sciences. Suggested activities and experiments that the student can do are also included.…

The Platinum Historical Literacy Class. A 353 Project Final Report.

ERIC Educational Resources Information Center

Schuylkill Intermediate Unit #29, Mar Lin, PA.

A pilot adult basic education (ABE) language experience curriculum was designed and implemented with older adults in Schuylkill County, Pennsylvania. The objectives were to improve writing skills, to increase self-esteem, and to create a historical record of the area as seen through the eyes of the average older citizen who had lived through many…

High School Credentialing in Wisconsin. Forum: Responding to Changes in High School Equivalency Testing

ERIC Educational Resources Information Center

Panzer, Willa; Johnson, Mark; Lewis, Beth

2015-01-01

Wisconsinites interested in pursuing a high school credential rely on the unique collaboration between the State GED Administrator at the Wisconsin Department of Instruction (DPI), the State Director of Adult Basic Education (ABE) and staff of the Wisconsin Technical College System (WTCS), and the many providers of instruction throughout the…

Good Citizens through Literacy Network. A 353 Project Final Report.

ERIC Educational Resources Information Center

Leto, Lyn

An adult basic education (ABE) class was developed to help parolees of the Schuylkill County Prison develop reading, writing, and communication skills and use those skills in a community service project aimed at helping at-risk youth. Parolees were recruited into the project through county parole officers, and their reading, writing, self-esteem,…

Radical Conversations: Part One Social-Constructivist Methods in the ABE Classroom

ERIC Educational Resources Information Center

Muth, Bill

2008-01-01

For the past 40 years adult learning theory has stressed the need for adults to share in the planning of their own learning and socially construct new knowledge by building on their background knowledge and life experiences. Despite growing acceptance of social-constructivist pedagogies in community-based literacy programs and even corporate…

Increasing access to learning for the adult basic education learner with learning disabilities: evidence-based accommodation research.

PubMed

Gregg, Noel

2012-01-01

Accommodating adult basic education (ABE) learners with learning disabilities (LD) is common practice across many instructional, testing, and work settings. However, the results from this literature search indicate that very few empirically based studies are available to support or reject the effectiveness of a great deal of accommodation implementation. In addition, in light of the profound changes to literacy taking place in today's digital, networked, and multimodal world, technology is redefining traditional concepts of accessibility and accommodation.

Aqueous extracts from Uncaria tomentosa (Willd. ex Schult.) DC. reduce bronchial hyperresponsiveness and inflammation in a murine model of asthma.

PubMed

Azevedo, Bruna Cestari; Morel, Lucas Junqueira Freitas; Carmona, Fábio; Cunha, Thiago Mattar; Contini, Silvia Helena Taleb; Delprete, Piero Giuseppe; Ramalho, Fernando Silva; Crevelin, Eduardo; Bertoni, Bianca Waléria; França, Suzelei Castro; Borges, Marcos Carvalho; Pereira, Ana Maria Soares

2018-05-23

Uncaria tomentosa (Willd. Ex Schult) DC is used by indigenous tribes in the Amazonian region of Central and South America to treat inflammation, allergies and asthma. The therapeutic properties of U. tomentosa have been attributed to the presence of tetracyclic and pentacyclic oxindole alkaloids and to phenolic acids. To characterize aqueous bark extracts (ABE) and aqueous leaf extracts (ALE) of U. tomentosa and to compare their anti-inflammatory effects. Constituents of the extracts were identified by ultra performance liquid chromatography-mass spectrometry. Anti-inflammatory activities were assessed in vitro by exposing lipopolysaccharide-stimulated macrophage cells (RAW264.7-Luc) to ABE, ALE and standard mitraphylline. In vivo assays were performed using a murine model of ovalbumin (OVA)-induced asthma. OVA-sensitized animals were treated with ABE or ALE while controls received dexamethasone or saline solution. Bronchial hyperresponsiveness, production of Th1 and Th2 cytokines, total and differential counts of inflammatory cells in the bronchoalveolar lavage (BAL) and lung tissue were determined. Mitraphylline, isomitraphylline, chlorogenic acid and quinic acid were detected in both extracts, while isorhyncophylline and rutin were detected only in ALE. ABE, ALE and mitraphylline inhibited the transcription of nuclear factor kappa-B in cell cultures, ALE and mitraphylline reduced the production of interleukin (IL)-6, and mitraphylline reduced production of tumor necrosis factor-alpha. Treatment with ABE and ALE at 50 and 200 mg kg -1 , respectively, reduced respiratory elastance and tissue damping and elastance. ABE and ALE reduced the number of eosinophils in BAL, while ALE at 200 mg kg -1 reduced the levels of IL-4 and IL-5 in the lung homogenate. Peribronchial inflammation was significantly reduced by treatment with ABE and ALE at 50 and 100 mg kg -1 respectively. The results clarify for the first time the anti-inflammatory activity of U. tomentosa in a murine model of asthma. Although ABE and ALE exhibited distinct chemical compositions, both extracts inhibited the production of pro-inflammatory cytokines in vitro. In vivo assays revealed that ABE was more effective in treating asthmatic inflammation while ALE was more successful in controlling respiratory mechanics. Both extracts may have promising applications in the phytotherapy of allergic asthma. Copyright © 2018 Elsevier B.V. All rights reserved.

High Solid Fed-batch Butanol Fermentation with Simultaneous Product Recovery: Part II - Process Integration.

PubMed

Qureshi, Nasib; Klasson, K Thomas; Saha, Badal C; Liu, Siqing

2018-04-25

In these studies liquid hot water (LHW) pretreated and enzymatically hydrolyzed Sweet Sorghum Bagasse (SSB) hydrolyzates were fermented in a fed-batch reactor. As reported in the preceding paper, the culture was not able to ferment the hydrolyzate I in a batch process due to presence of high level of toxic chemicals, in particular acetic acid released from SSB during the hydrolytic process. To be able to ferment the hydrolyzate I obtained from 250 gL -1 SSB hydrolysis, a fed-batch reactor with in-situ butanol recovery was devised. The process was started with the hydrolyzate II and when good cell growth and vigorous fermentation were observed, the hydrolyzate I was slowly fed to the reactor. In this manner the culture was able to ferment all the sugars present in both the hydrolyzates to acetone butanol ethanol (ABE). In a control batch reactor in which ABE was produced from glucose, ABE productivity and yield of 0.42 gL -1 h -1 and 0.36 were obtained, respectively. In the fed-batch reactor fed with SSB hydrolyzates these productivity and yield values were 0.44 gL -1 h -1 and 0.45, respectively. ABE yield in the integrated system was high due to utilization of acetic acid to convert to ABE. In summary we were able to utilize both the hydrolyzates obtained from LHW pretreated and enzymatically hydrolyzed SSB (250 gL -1 ) and convert them to ABE. Complete fermentation was possible due to simultaneous recovery of ABE by vacuum. This article is protected by copyright. All rights reserved. © 2018 American Institute of Chemical Engineers.

High acetone-butanol-ethanol production in pH-stat co-feeding of acetate and glucose.

PubMed

Gao, Ming; Tashiro, Yukihiro; Wang, Qunhui; Sakai, Kenji; Sonomoto, Kenji

2016-08-01

We previously reported the metabolic analysis of butanol and acetone production from exogenous acetate by (13)C tracer experiments (Gao et al., RSC Adv., 5, 8486-8495, 2015). To clarify the influence of acetate on acetone-butanol-ethanol (ABE) production, we first performed an enzyme assay in Clostridium saccharoperbutylacetonicum N1-4. Acetate addition was found to drastically increase the activities of key enzymes involved in the acetate uptake (phosphate acetyltransferase and CoA transferase), acetone formation (acetoacetate decarboxylase), and butanol formation (butanol dehydrogenase) pathways. Subsequently, supplementation of acetate during acidogenesis and early solventogenesis resulted in a significant increase in ABE production. To establish an efficient ABE production system using acetate as a co-substrate, several shot strategies were investigated in batch culture. Batch cultures with two substrate shots without pH control produced 14.20 g/L butanol and 23.27 g/L ABE with a maximum specific butanol production rate of 0.26 g/(g h). Furthermore, pH-controlled (at pH 5.5) batch cultures with two substrate shots resulted in not only improved acetate consumption but also a further increase in ABE production. Finally, we obtained 15.13 g/L butanol and 24.37 g/L ABE at the high specific butanol production rate of 0.34 g/(g h) using pH-stat co-feeding method. Thus, in this study, we established a high ABE production system using glucose and acetate as co-substrates in a pH-stat co-feeding system with C. saccharoperbutylacetonicum N1-4. Copyright © 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Agaricus blazei Extract Induces Apoptosis through ROS-Dependent JNK Activation Involving the Mitochondrial Pathway and Suppression of Constitutive NF-κB in THP-1 Cells

PubMed Central

Kim, Mun-Ock; Moon, Dong-Oh; Jung, Jin Myung; Lee, Won Sup; Choi, Yung Hyun; Kim, Gi-Young

2011-01-01

Agaricus blazei is widely accepted as a traditional medicinal mushroom, and it has been known to exhibit immunostimulatory and anti-cancer activity. However, the apoptotic mechanism in cancer cells is poorly understood. In this study, we have investigated whether A. blazei extract (ABE) exerts antiproliferative and apoptotic effects in human leukemic THP-1 cells. We observed that ABE-induced apoptosis is associated with the mitochondrial pathway, which is mediated by reactive oxygen species (ROS) generation and prolonged c-Jun N-terminal kinase (JNK) activation. In addition, the ABE treatment resulted in the accumulation of cytochrome c in the cytoplasm, an increase in caspase activity, and an upregulation of Bax and Bad. With those results in mind, we found that ABE decreases constitutive NF-κB activation and NF-κB-regulated gene products such as IAP-1 and -2. We concluded that ABE induces apoptosis with ROS-dependent JNK activation and constitutive activated NF-κB inhibition in THP-1 cells. PMID:19861509

Production of butanol from starch-based waste packing peanuts and agricultural waste.

PubMed

Jesse, T W; Ezeji, T C; Qureshi, N; Blaschek, H P

2002-09-01

We examined the fermentation of starch-based packing peanuts and agricultural wastes as a source of fermentable carbohydrates using Clostridium beijerinckii BA101. Using semidefined P2 medium containing packing peanuts and agricultural wastes, instead of glucose as a carbohydrate source, we measured characteristics of the fermentation including solvent production, productivity, and yield. With starch as substrate (control), the culture produced 24.7 g l(-1) acetone-butanol-ethanol (ABE), while with packing peanuts it produced 21.7 g l(-1) total ABE with a productivity of 0.20 g l(-1) h(-1) and a solvent (ABE) yield of 0.37. Cell growth in starch, packing peanuts, and agricultural wastes medium was different, possibly due to the different nature of these substrates. Using model agricultural waste, 20.3g l(-1) ABE was produced; when using actual waste, 14.8 g l(-1) ABE was produced. The use of inexpensive substrates will increase the economic viability of the conversion of biomass to butanol, and can provide new markets for these waste streams.

Production of acetone butanol (AB) from liquefied corn starch, a commercial substrate, using Clostridium beijerinckii coupled with product recovery by gas stripping.

PubMed

Ezeji, Thaddeus C; Qureshi, Nasib; Blaschek, Hans P

2007-12-01

A potential industrial substrate (liquefied corn starch; LCS) has been employed for successful acetone butanol ethanol (ABE) production. Fermentation of LCS (60 g l(-1)) in a batch process resulted in the production of 18.4 g l(-1) ABE, comparable to glucose: yeast extract based medium (control experiment, 18.6 g l(-1) ABE). A batch fermentation of LCS integrated with product recovery resulted in 92% utilization of sugars present in the feed. When ABE was recovered by gas stripping (to relieve inhibition) from the fed-batch reactor fed with saccharified liquefied cornstarch (SLCS), 81.3 g l(-1) ABE was produced compared to 18.6 g l(-1) (control). In this integrated system, 225.8 g l(-1) SLCS sugar (487 % of control) was consumed. In the absence of product removal, it is not possible for C. beijerinckii BA101 to utilize more than 46 g l(-1) glucose. A combination of fermentation of this novel substrate (LCS) to butanol together with product recovery by gas stripping may economically benefit this fermentation.

Recent advances on conversion and co-production of acetone-butanol-ethanol into high value-added bioproducts.

PubMed

Xin, Fengxue; Dong, Weiliang; Jiang, Yujia; Ma, Jiangfeng; Zhang, Wenming; Wu, Hao; Zhang, Min; Jiang, Min

2018-06-01

Butanol is an important bulk chemical and has been regarded as an advanced biofuel. Large-scale production of butanol has been applied for more than 100 years, but its production through acetone-butanol-ethanol (ABE) fermentation process by solventogenic Clostridium species is still not economically viable due to the low butanol titer and yield caused by the toxicity of butanol and a by-product, such as acetone. Renewed interest in biobutanol as a biofuel has spurred technological advances to strain modification and fermentation process design. Especially, with the development of interdisciplinary processes, the sole product or even the mixture of ABE produced through ABE fermentation process can be further used as platform chemicals for high value added product production through enzymatic or chemical catalysis. This review aims to comprehensively summarize the most recent advances on the conversion of acetone, butanol and ABE mixture into various products, such as isopropanol, butyl-butyrate and higher-molecular mass alkanes. Additionally, co-production of other value added products with ABE was also discussed.

Enhanced sugar production from pretreated barley straw by additive xylanase and surfactants in enzymatic hydrolysis for acetone-butanol-ethanol fermentation.

PubMed

Yang, Ming; Zhang, Junhua; Kuittinen, Suvi; Vepsäläinen, Jouko; Soininen, Pasi; Keinänen, Markku; Pappinen, Ari

2015-01-01

This study aims to improve enzymatic sugar production from dilute sulfuric acid-pretreated barley straw for acetone-butanol-ethanol (ABE) fermentation. The effects of additive xylanase and surfactants (polyethylene glycol [PEG] and Tween) in an enzymatic reaction system on straw hydrolysis yields were investigated. By combined application of 2g/100g dry-matter (DM) xylanase and PEG 4000, the glucose yield was increased from 53.2% to 86.9% and the xylose yield was increased from 36.2% to 70.2%, which were considerably higher than results obtained with xylanase or surfactant alone. The ABE fermentation of enzymatic hydrolysate produced 10.8 g/L ABE, in which 7.9 g/L was butanol. The enhanced sugar production increased the ABE yield from 93.8 to 135.0 g/kg pretreated straw. The combined application of xylanase and surfactants has a large potential to improve sugar production from barley straw pretreated with a mild acid and that the hydrolysate showed good fermentability in ABE production. Copyright © 2015 Elsevier Ltd. All rights reserved.

Learning Lasts a Lifetime. Report of the Colorado Business Commission on Adult Learning.

ERIC Educational Resources Information Center

Colorado Office of the Governor, Denver.

A commission of business leaders appointed by the governor of Colorado studied adult basic education (ABE) in the state in order to determine its value to the citizens of the state and to the state's future. Throughout a 6-month period, the commission discussed issues pertaining to the field with private providers, adult learners, and government…

ABE/GED Instruction of High School Dropouts. "353" Special Demonstration Project.

ERIC Educational Resources Information Center

Norwood, Dianne

One hundred individuals who dropped out of high school in Lawrence County, Alabama, during the 1986-87 school year were studied in order to help formulate ways schools could prevent students from dropping out and ideas on how to create advertising about available adult education to which such dropouts would be receptive. A former in-home adult…

Pre-Service Training Model for TESOL/ABE Teachers and Teacher-Aides.

ERIC Educational Resources Information Center

Southwestern Cooperative Educational Lab., Albuquerque, NM.

Developed by the Proteus Adult Education Team of Visalia, Calif., this preservice training model, the result of a project for teachers and teacher-aides of Mexican American adult students, recommends a structure of 30 hours intensive training conducted over a period of 2 weeks (to be followed by weekly 2 1/2-hour inservice sessions). This booklet…

On the Effect of an Anisotropy-Resolving Subgrid-Scale Model on Turbulent Vortex Motions

DTIC Science & Technology

2014-09-19

sense, the model by Abe (2013) can be named the ”stabilized mixed model” ( SMM , hereafter). Furthermore, considering the basic concept of the mixed model...with SMM . Further investigations of this ex- tended anisotropic SGS model will be necessary in fu- ture studies. 3 Computational Conditions Although the...basic capability of the SMM was val- idated by application to some test cases (Abe, 2013; Abe 2014), there still remain several points to be fur

Rogue Community College Student Satisfaction Survey, Winter 2000. Management Report: ABE/GED Program.

ERIC Educational Resources Information Center

Wild, Nancy

The report discusses the winter 2000 student satisfaction survey at Rogue Community College (RCC) (Oregon). The annual survey is an important tool by which the college measures its achievement in the areas of services, classes, and facilities. The primary purpose of the study is to obtain feedback from attending students regarding the issues that…

Adults Must Be College-Ready Too: ABE-to-College Transition Project Inspires Lives

ERIC Educational Resources Information Center

Wilson, Blenda J.

2006-01-01

In this article, the author shares the voices of adults whose lives have been changed by their enrollment in a set of innovative college readiness programs geared toward adults. These women and men have overcome obstacles between them and college that they believed were insurmountable--fear, inadequate academic preparation, lack of information…

Detecting and Identifying Organic Molecules in Space - The AstroBiology Explorer (ABE) MIDEX Mission Concept

NASA Technical Reports Server (NTRS)

Sandford, Scott A.

2001-01-01

Infrared spectroscopy in the 2.5-16 micron (4000-625/cm) range is a principle means by which organic compounds are detected and identified in space. Ground-based, airborne, and spaceborne IR spectral studies have already demonstrated that a significant fraction of the carbon in the interstellar medium (ISM) resides in the form of complex organic molecular species. Unfortunately, neither the distribution of these materials nor their genetic and evolutionary relationships with each other or their environments are well understood. The Astrobiology Explorer (ABE) is a MIDEX (Medium-class Explorer) mission concept currently under study at NASA's Ames Research Center in collaboration with Ball Aerospace and Technologies Corporation. ABE will conduct IR spectroscopic observations to address outstanding important problems in astrobiology, astrochemistry, and astrophysics. The core observational program would make fundamental scientific progress in understanding (1) the evolution of ices and organic matter in dense molecular clouds and young forming stellar systems, (2) the chemical evolution of organic molecules in the ISM as they transition from AGB outflows to planetary nebulae to the general diffuse ISM to H II regions and dense clouds, (3) the distribution of organics in the diffuse ISM, (4) the nature of organics in the Solar System (in comets, asteroids, satellites), and (5) the nature and distribution of organics in local galaxies. Both the scientific goals of the mission and how they would be achieved will be discussed.

Identifying Organic Molecules in Space: The AstroBiology Explorer (ABE) MIDEX Mission Concept

NASA Technical Reports Server (NTRS)

Sandford, Scott A.; Allamandola, Louis; Bregman, Jesse; Ennico, Kimberly; Greene, Thomas; Hudgins, Douglas; Strecker, Donald; DeVincenzi, Donald (Technical Monitor)

2001-01-01

Infrared spectroscopy in the 2.5-16 micron range is a principle means by which organic compounds are detected and identified in space. Ground-based, airborne, and spaceborne IR spectral studies have already demonstrated that a significant fraction of the carbon in the interstellar medium (ISM) resides in the form of complex organic molecular species. Unfortunately, neither the distribution of these materials nor their genetic and evolutionary relationships with each other or their environments are well understood. The Astrobiology Explorer (ABE) is a MIDEX mission concept currently under study at NASA's Ames Research Center in collaboration with Ball Aerospace and Technologies Corporation. ABE will conduct IR spectroscopic observations to address outstanding important problems in astrobiology, astrochemistry, and astrophysics. The core observational program would make fundamental scientific progress in understanding (1) the evolution of ices and organic matter in dense molecular clouds and young forming stellar systems, (2) the chemical evolution of organic molecules in the ISM as they transition from AGB outflows to planetary nebulae to the general diffuse ISM to H II regions and dense clouds, (3) the distribution of organics in the diffuse ISM, (4) the nature of organics in the Solar System (in comets, asteroids, satellites), and (5) the nature and distribution of organics in local galaxies. The technical considerations of achieving these science objectives in a MIDEX-sized mission will be described.

Detecting and Identifying Organic Molecules in Space: The AstroBiology Explorer (ABE) MIDEX Mission Concept

NASA Technical Reports Server (NTRS)

Sandford, Scott A.; DeVincenzi, Donald (Technical Monitor)

2001-01-01

Infrared spectroscopy in the 2.5-16 microns (4000-625/cm) range is a principle means by which organic compounds are detected and identified in space. Ground-based, airborne, and spaceborne IR spectral studies have already demonstrated that a significant fraction of the carbon in the interstellar medium (ISM) resides in the form of complex organic molecular species. Unfortunately, neither the distribution of these materials nor their genetic and evolutionary relationships with each other or their environments are well understood. The Astrobiology Explorer (ABE) is a MIDEX (Medium-class Explorer) mission concept currently under study at NASA's Ames Research Center in collaboration with Ball Aerospace and Technologies Corporation. ABE will conduct IR spectroscopic observations to address outstanding important problems in astrobiology, astrochemistry, and astrophysics. The core observational program would make fundamental scientific progress in understanding (1) the evolution of ices and organic matter in dense molecular clouds and young forming stellar systems, (2) the chemical evolution of organic molecules in the ISM as they transition from AGB outflows to planetary nebulae to the general diffuse ISM to H II regions and dense clouds, (3) the distribution of organics in the diffuse ISM, (4) the nature of organics in the Solar System (in comets, asteroids, satellites), and (5) the nature and distribution of organics in local galaxies. Both the scientific goals of the mission and how they would be achieved will be discussed.

Reading component skills of learners in adult basic education.

PubMed

MacArthur, Charles A; Konold, Timothy R; Glutting, Joseph J; Alamprese, Judith A

2010-01-01

The purposes of this study were to investigate the reliability and construct validity of measures of reading component skills with a sample of adult basic education (ABE) learners, including both native and nonnative English speakers, and to describe the performance of those learners on the measures. Investigation of measures of reading components is needed because available measures were neither developed for nor normed on ABE populations or with nonnative speakers of English. The study included 486 students, 334 born or educated in the United States (native) and 152 not born or educated in the United States (nonnative) but who spoke English well enough to participate in English reading classes. All students had scores on 11 measures covering five constructs: decoding, word recognition, spelling, fluency, and comprehension. Confirmatory factor analysis (CFA) was used to test three models: a two-factor model with print and meaning factors; a three-factor model that separated out a fluency factor; and a five-factor model based on the hypothesized constructs. The five-factor model fit best. In addition, the CFA model fit both native and nonnative populations equally well without modification, showing that the tests measure the same constructs with the same accuracy for both groups. Group comparisons found no difference between the native and nonnative samples on word recognition, but the native sample scored higher on fluency and comprehension and lower on decoding than did the nonnative sample. Students with self-reported learning disabilities scored lower on all reading components. Differences by age and gender were also analyzed.

Application of continuous substrate feeding to the ABE fermentation: Relief of product inhibition using extraction, perstraction, stripping, and pervaporation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Qureshi, N.; Maddox, I.S.; Friedl, A.

1992-09-01

The technique of continuous substrate feeding has been applied to the batch fermentation process using freely suspended cells, for ABE (acetone-butanol-ethanol) production. To avoid the product inhibition which normally restricts ABE production to less than 20 g/L and sugar utilization to 60 g/L, a product removal technique has been integrated into the fermentation process. The techniques investigated were liquid-liquid extraction, perstraction, gas-stripping, and pervaporation. By using a substrate of whey permeate, the reactor productivity has been improved over that observed in a traditional batch fermentation, while equivalent lactose utilization and ABE production values of 180 g and 69 g, respectively,more » have been achieved in a 1-L culture volume. 17 refs., 14 figs., 5 tabs.« less

Mathematical models of ABE fermentation: review and analysis.

PubMed

Mayank, Rahul; Ranjan, Amrita; Moholkar, Vijayanand S

2013-12-01

Among different liquid biofuels that have emerged in the recent past, biobutanol produced via fermentation processes is of special interest due to very similar properties to that of gasoline. For an effective design, scale-up, and optimization of the acetone-butanol-ethanol (ABE) fermentation process, it is necessary to have insight into the micro- and macro-mechanisms of the process. The mathematical models for ABE fermentation are efficient tools for this purpose, which have evolved from simple stoichiometric fermentation equations in the 1980s to the recent sophisticated and elaborate kinetic models based on metabolic pathways. In this article, we have reviewed the literature published in the area of mathematical modeling of the ABE fermentation. We have tried to present an analysis of these models in terms of their potency in describing the overall physiology of the process, design features, mode of operation along with comparison and validation with experimental results. In addition, we have also highlighted important facets of these models such as metabolic pathways, basic kinetics of different metabolites, biomass growth, inhibition modeling and other additional features such as cell retention and immobilized cultures. Our review also covers the mathematical modeling of the downstream processing of ABE fermentation, i.e. recovery and purification of solvents through flash distillation, liquid-liquid extraction, and pervaporation. We believe that this review will be a useful source of information and analysis on mathematical models for ABE fermentation for both the appropriate scientific and engineering communities.

Enhancement of n-butanol production by in situ butanol removal using permeating-heating-gas stripping in acetone-butanol-ethanol fermentation.

PubMed

Chen, Yong; Ren, Hengfei; Liu, Dong; Zhao, Ting; Shi, Xinchi; Cheng, Hao; Zhao, Nan; Li, Zhenjian; Li, Bingbing; Niu, Huanqing; Zhuang, Wei; Xie, Jingjing; Chen, Xiaochun; Wu, Jinglan; Ying, Hanjie

2014-07-01

Butanol recovery from acetone-butanol-ethanol (ABE) fed-batch fermentation using permeating-heating-gas was determined in this study. Fermentation was performed with Clostridium acetobutylicum B3 in a fibrous bed bioreactor and permeating-heating-gas stripping was used to eliminate substrate and product inhibition, which normally restrict ABE production and sugar utilization to below 20 g/L and 60 g/L, respectively. In batch fermentation (without permeating-heating-gas stripping), C. acetobutylicum B3 utilized 60 g/L glucose and produced 19.9 g/L ABE and 12 g/L butanol, while in the integrated process 290 g/L glucose was utilized and 106.27 g/L ABE and 66.09 g/L butanol were produced. The intermittent gas stripping process generated a highly concentrated condensate containing approximately 15% (w/v) butanol, 4% (w/v) acetone, a small amount of ethanol (<1%), and almost no acids, resulting in a highly concentrated butanol solution [∼ 70% (w/v)] after phase separation. Butanol removal by permeating-heating-gas stripping has potential for commercial ABE production. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

Bcl-2 and caspase-3 are major regulators in Agaricus blazei-induced human leukemic U937 cell apoptosis through dephoshorylation of Akt.

PubMed

Jin, Cheng-Yun; Moon, Dong-Oh; Choi, Yung Hyun; Lee, Jae-Dong; Kim, Gi-Young

2007-08-01

Agaricus blazei is a medicinal mushroom that possesses antimetastatic, antitumor, antimutagenic, and immunostimulating effects. However, the molecular mechanisms involved in A. blazei-mediated apoptosis remain unclear. In the present study, to elucidate the role of the Bcl-2 in A. blazei-mediated apoptosis, U937 cells were transfected with either empty vector (U937/vec) or vector containing cDNA encoding full-length Bcl-2 (U937/Bcl-2). As compared with U937/vec, U937/Bcl-2 cells exhibited a 4-fold greater expression of Bcl-2. Treatment of U937/vec with 1.0-4.0 mg/ml of A. blazei extract (ABE) for 24 h resulted in a significant induction of morphologic features indicative of apoptosis. In contrast, U937/Bcl-2 exposed to the same ABE treatment only exhibited a slight induction of apoptotic features. ABE-induced apoptosis was accompanied by downregulation of antiapoptotic proteins such as X-linked inhibitor of apoptosis protein (XIAP), inhibitor of apoptosis protein (cIAP)-2 and Bcl-2, activation of caspase-3, and cleavage of poly(ADP-ribose)polymerase (PARP). Ectopic expression of Bcl-2 was associated with significantly induced expression of antiapoptotic proteins, such as cIAP-2 and Bcl-2, but not XIAP. Ectopic expression of Bcl-2 also reduced caspase-3 activation and PARP cleavage in ABE treated U937 cells. Furthermore, treatment with the caspase-3 inhibitor z-DEVD-fmk was sufficient to restore cell viability following ABE treatment. This increase in viability was ascribed to downregulation of caspase-3 and blockage of PARP and PLC-gamma cleavage. ABE also triggered the downregulation of Akt, and combined treatment with LY294002 (an inhibitor of Akt) significantly decreased cell viability. The results indicated that major regulators of ABE-induced apoptosis in human leukemic U937 cells are Bcl-2 and caspase-3, which are associated with dephosphorylation of the Akt signal pathway.

A novel in situ gas stripping-pervaporation process integrated with acetone-butanol-ethanol fermentation for hyper n-butanol production.

PubMed

Xue, Chuang; Liu, Fangfang; Xu, Mengmeng; Zhao, Jingbo; Chen, Lijie; Ren, Jiangang; Bai, Fengwu; Yang, Shang-Tian

2016-01-01

Butanol is considered as an advanced biofuel, the development of which is restricted by the intensive energy consumption of product recovery. A novel two-stage gas stripping-pervaporation process integrated with acetone-butanol-ethanol (ABE) fermentation was developed for butanol recovery, with gas stripping as the first-stage and pervaporation as the second-stage using the carbon nanotubes (CNTs) filled polydimethylsiloxane (PDMS) mixed matrix membrane (MMM). Compared to batch fermentation without butanol recovery, more ABE (27.5 g/L acetone, 75.5 g/L butanol, 7.0 g/L ethanol vs. 7.9 g/L acetone, 16.2 g/L butanol, 1.4 g/L ethanol) were produced in the fed-batch fermentation, with a higher butanol productivity (0.34 g/L · h vs. 0.30 g/L · h) due to reduced butanol inhibition by butanol recovery. The first-stage gas stripping produced a condensate containing 155.6 g/L butanol (199.9 g/L ABE), which after phase separation formed an organic phase containing 610.8 g/L butanol (656.1 g/L ABE) and an aqueous phase containing 85.6 g/L butanol (129.7 g/L ABE). Fed with the aqueous phase of the condensate from first-stage gas stripping, the second-stage pervaporation using the CNTs-PDMS MMM produced a condensate containing 441.7 g/L butanol (593.2 g/L ABE), which after mixing with the organic phase from gas stripping gave a highly concentrated product containing 521.3 g/L butanol (622.9 g/L ABE). The outstanding performance of CNTs-PDMS MMM can be attributed to the hydrophobic CNTs giving an alternative route for mass transport through the inner tubes or along the smooth surface of CNTs. This gas stripping-pervaporation process with less contaminated risk is thus effective in increasing butanol production and reducing energy consumption. © 2015 Wiley Periodicals, Inc.

NASA Administrator James Webb and Lewis Director Abe Silverstein

NASA Image and Video Library

1961-12-21

National Aeronautics and Space Administration (NASA) Administrator James Webb toured the new Plum Brook Reactor Facility in December 1961 with Abe Silverstein, the newly appointed Director of the Lewis Research Center. The 60-megawatt test reactor was built on 500 acres of the former Plum Brook Ordnance Works in Sandusky, Ohio. After nearly five years of construction, the facility went critical for the first time in June 1961. In late 1957 Hugh Dryden requested Silverstein’s assistance in creating the new space agency. After several months of commuting, Silverstein transferred to Headquarters in May 1958. Silverstein was a critical member of a team that devised a fiscal year 1960 budget and began planning missions. When NASA officially began operation on October 1, 1958, Silverstein was third in command. He directed mission planning, spacecraft design, launch operations, manned space missions, and unmanned probes. James Webb, named NASA administrator on January 7, 1961, sought to have those working on Apollo at the NASA centers report to a new Headquarters program office, not to the head of the Apollo Program. Silverstein requested to be appointed to the vacant center director position in Cleveland. He officially returned as director of the Lewis Research Center on November 1, 1961.

Acetone-butanol-ethanol competitive sorption simulation from single, binary, and ternary systems in a fixed-bed of KA-I resin.

PubMed

Wu, Jinglan; Zhuang, Wei; Ying, Hanjie; Jiao, Pengfei; Li, Renjie; Wen, Qingshi; Wang, Lili; Zhou, Jingwei; Yang, Pengpeng

2015-01-01

Separation of butanol based on sorption methodology from acetone-butanol-ethanol (ABE) fermentation broth has advantages in terms of biocompatibility and stability, as well as economy, and therefore gains much attention. In this work a chromatographic column model based on the solid film linear driving force approach and the competitive Langmuir isotherm equations was used to predict the competitive sorption behaviors of ABE single, binary, and ternary mixture. It was observed that the outlet concentration of weaker retained components exceeded the inlet concentration, which is an evidence of competitive adsorption. Butanol, the strongest retained component, could replace ethanol almost completely and also most of acetone. In the end of this work, the proposed model was validated by comparison of the experimental and predicted ABE ternary breakthrough curves using the real ABE fermentation broth as a feed solution. © 2014 American Institute of Chemical Engineers.

Ar-40/Ar-39 and U-Th-Pb dating of separated clasts from the Abee E4 chondrite

NASA Technical Reports Server (NTRS)

Bogard, D. D.; Unruh, D. M.; Tatsumoto, M.

1983-01-01

Ar-40/Ar-39 and U-Th-Pb are investigated for three clasts from the Abee (E4) enstatite chondrite, yielding Ar-40/Ar-39 plateau ages (and/or maximum ages) of 4.5 Gy, while two of the clasts give average ages of 4.4 Gy. The 4.4-4.5 Gy range does not resolve possible age differences among the clasts. The U-Th-Pb data are consistent with the interpretation that initial clast formation occurred 4.58 Gy ago, and that the clasts have since remained closed systems which have been contaminated with terrestrial Pb. The thermal history of Abee deduced from Ar data seems consistent with that deduced from magnetic data, suggesting that various Abee components experienced separate histories until brecciation no later than 4.4 Gy ago, experiencing no significant subsequent heating.

The acid-base impact of free water removal from, and addition to, plasma.

PubMed

Haskins, Steve C; Hopper, Kate; Rezende, Marlis L

2006-03-01

Water, compared with plasma at a pH of 7.4, is a weak acid. The addition of free water to a patient should have an acidifying effect (dilutional acidosis) and the removal of it, an alkalinizing effect (concentrational alkalosis). The specific effects of free water loss or gain in a relatively complex fluid such as plasma has, to the authors' knowledge, not been reported. This information would be useful in the interpretation of the effect of changes in free water in patients. Plasma samples from goats were either evaporated in a tonometer to 80% of baseline volume or hydrated by the addition of distilled water to 120% of baseline volume. The pH and partial pressure of carbon dioxide, sodium, potassium, ionized calcium, chloride, lactate, phosphorous, albumin, and total protein concentrations were measured. Actual base excess (ABE), standard bicarbonate, anion gap, strong ion difference, strong ion gap, unmeasured anions, and the effects of sodium, chloride, phosphate, and albumin changes on ABE were calculated. Most parameters changed 20% in proportion to the magnitude of dehydration or hydration. Bicarbonate concentration, however, increased only 11% in the evaporation trial and decreased only -2% in the dehydration trial. The evaporation trial was associated with a mild, but significant, metabolic alkalotic effect (ABE increased 3.2 mM/L), whereas the hydration trial was associated with a slight, insignificant metabolic acidotic effect (ABE decreased only 0.6 mM/L). The calculated free water ABE effect (change in sodium concentration) was offset by opposite changes in calculated chloride, lactate, phosphate, and albumin ABE effects.

Periodic peristalsis increasing acetone-butanol-ethanol productivity during simultaneous saccharification and fermentation of steam-exploded corn straw.

PubMed

Li, Jingwen; Wang, Lan; Chen, Hongzhang

2016-11-01

The acetone-butanol-ethanol (ABE) fermentation of lignocellulose at high solids content has recently attracted extensive attention. However, the productivity of high solids ABE fermentation of lignocellulose is typically low in traditional processes due to the lack of efficient intensifying methods. In the present study, periodic peristalsis, a novel intensifying method, was applied to improve ABE production by the simultaneous saccharification and fermentation (SSF) of steam-exploded corn straw using Clostridium acetobutylicum ATCC824. The ABE concentration and the ABE productivity of SSF at a solids content of 17.5% (w/w) with periodic peristalsis were 17.1 g/L and 0.20 g/(L h), respectively, which were higher than those obtained under static conditions (15.2 g/L and 0.14 g/(L h)). The initial sugar conversion rate over the first 12 h with periodic peristalsis was 4.67 g/(L h) at 10 FPU/g cellulase dosage and 15% (w/w) solids content, an increase of 49.7% compared with the static conditions. With periodic peristalsis, the period of batch fermentation was shortened from 108 h to 84 h. The optimal operating regime was a low frequency (6 h -1 ) of periodic peristalsis in the acid-production phase (0-48 h) of SSF. Therefore, periodic peristalsis should be an effective intensifying method to increase the productivity of ABE fermentation at high solids content. Copyright © 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

More than a "Basic Skill": Breaking down the Complexities of Summarizing for ABE/ESL Learners

ERIC Educational Resources Information Center

Ouellette-Schramm, Jennifer

2015-01-01

This article describes the complex cognitive and linguistic challenges of summarizing expository text at vocabulary, syntactic, and rhetorical levels. It then outlines activities to help ABE/ESL learners develop corresponding skills.

Divided attention enhances explicit but not implicit conceptual memory: an item-specific account of the attentional boost effect.

PubMed

Spataro, Pietro; Mulligan, Neil W; Bechi Gabrielli, Giulia; Rossi-Arnaud, Clelia

2017-02-01

The Attentional Boost Effect (ABE) refers to the counterintuitive finding that words encoded with to-be-responded targets in a divided-attention condition are remembered better than words encoded with distractors. Previous studies suggested that the ABE-related enhancement of verbal memory depends upon the activation of abstract lexical representations. In the present study, we extend this hypothesis by embedding it in the context of a broader perspective, which proposes that divided attention in the ABE paradigm affects item-specific, but not relational, processing. To this purpose, we examined the ABE in the matched tasks of category-cued recall (CCRT: explicit memory) and category exemplar generation (CEGT: implicit memory). In addition, study time was varied (500, 1500 or 4000 ms), to further determine whether the attentional boost manipulation could influence late-phase elaborative processing. In agreement with the predictions of the item-specific account, the results showed that exemplars encoded with targets were recalled better than exemplars encoded with distractors in the CCRT, but not in the CEGT. Moreover, performance in the CCRT increased with study time, whereas the size of the ABE-related enhancement tended to decrease, further confirming that this effect hinges upon early phase encoding processes.

In Vitro Longitudinal Relaxivity Profile of Gd(ABE-DTTA), an Investigational Magnetic Resonance Imaging Contrast Agent

PubMed Central

Varga-Szemes, Akos; Kiss, Pal; Rab, Andras; Suranyi, Pal; Lenkey, Zsofia; Simor, Tamas; Bryant, Robert G.; Elgavish, Gabriel A.

2016-01-01

Purpose MRI contrast agents (CA) whose contrast enhancement remains relatively high even at the higher end of the magnetic field strength range would be desirable. The purpose of this work was to demonstrate such a desired magnetic field dependency of the longitudinal relaxivity for an experimental MRI CA, Gd(ABE-DTTA). Materials and Methods The relaxivity of 0.5mM and 1mM Gd(ABE-DTTA) was measured by Nuclear Magnetic Relaxation Dispersion (NMRD) in the range of 0.0002 to 1T. Two MRI and five NMR instruments were used to cover the range between 1.5 to 20T. Parallel measurement of a Gd-DTPA sample was performed throughout as reference. All measurements were carried out at 37°C and pH 7.4. Results The relaxivity values of 0.5mM and 1mM Gd(ABE-DTTA) measured at 1.5, 3, and 7T, within the presently clinically relevant magnetic field range, were 15.3, 11.8, 12.4 s-1mM-1 and 18.1, 16.7, and 13.5 s-1mM-1, respectively. The control 4 mM Gd-DTPA relaxivities at the same magnetic fields were 3.6, 3.3, and 3.0 s-1mM-1, respectively. Conclusions The longitudinal relaxivity of Gd(ABE-DTTA) measured within the presently clinically relevant field range is three to five times higher than that of most commercially available agents. Thus, Gd(ABE-DTTA) could be a practical choice at any field strength currently used in clinical imaging including those at the higher end. PMID:26872055

Protective potential of Averrhoa bilimbi fruits in ameliorating the hepatic key enzymes in streptozotocin-induced diabetic rats.

PubMed

Kurup, Surya B; S, Mini

2017-01-01

Diabetes is a mutifactorial disease which leads to several complications. Currently available drug regimens for management of diabetes have certain drawbacks. Need for safer and effective medicines from natural sources having potent antidiabetic activity. Averrhoa bilimbi Linn. (Oxalidaceae) is a medicinal plant and is reported to possess hypoglycemic activity. To investigate the antidiabetic potential of Averrhoa bilimbi fruit extract in streptozotocin-induced diabetic rats. Diabetes was induced in male Sprague Dawley rats by single intraperitoneal injection of streptozotocin (STZ) (40mg/kg body weight). The diabetic rats were treated orally with ethyl acetate fraction of A. bilimbi fruits (ABE) (25mg/kg body weight) and metformin (100mg/kg body weight) by intragastric intubation for 60days. After 60days, the rats were sacrificed; blood, liver and pancreas were collected. Several indices such as blood glucose, plasma insulin, toxicity markers and the activities of carbohydrate-metabolizing enzymes were assayed. The phytochemicals present in the ABE was identified by gas chromatography-mass spectrometry analysis. ABE significantly (p<0.05) reduced the level of blood glucose and hepatic toxicity markers and increased plasma insulin in diabetic rats. ABE modulated the activities of carbohydrate-metabolizing enzymes, significantly increased the activities of hexokinase (59%) and pyruvate kinase (68%) and reduced the activities of glucose-6-phosphatase (32%) and fructose-1, 6-bisphosphatase (20%). The histological studies of the pancreas also supported our findings. The results were compared with metformin, a standard oral hypoglycemic drug. GC-MS analysis of ABE revealed the presence of 11 chemical constituents in the extract. ABE exerts its antidiabetic effect by promoting glucose metabolism via glycolysis and inhibiting hepatic endogenous glucose production via gluconeogenesis. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Final Technical Report for Grant # DE-FG02-06ER64169

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dr. Beat Schmid, PI

2007-07-13

The Atmospheric Radiation Measurement (ARM) program is funding this project to improve the methodology of ground-based remote sensing of the vertical distribution of aerosol and cloud optical properties, and their effect on atmospheric radiative transfer. Remotely-sensed and in situ observed aerosol, cloud physical, and optical properties collected during the May 2003 Aerosol Intensive Operational Period (AIOP) and the Aerosol Lidar Validation Experiment (ALIVE), conducted from September 11-22, 2005, are the basis for the investigation. We have used ground-based lidar, airborne sunphotometer and in situ measurements and other data to evaluate the vertical profile of aerosol properties. We have been pursuingmore » research in the following three areas: (1) Aerosol Best Estimate Product--Sensitivity Study: ARM is developing an Aerosol Best Estimate (ABE) Value Added Product (VAP) to provide aerosol optical properties at all times and heights above its sites. The ABE is used as input for the Broadband Heating Rate Profile (BBHRP) VAP, whose output will be used to evaluate the radiative treatment of aerosols and clouds in climate models. ARM has a need to assess how much detail is required for the ABE and if a useful ABE can be derived for the tropical and arctic climate research facilities (CRFs) where only limited aerosol information in the vertical is available. We have been determining the sensitivity of BBHRP to the vertical profile of aerosol optical properties used in ABE. (2) Vertically Resolved Aerosol and Cloud Radiative Properties over the Southern Great Plains (SGP): The AIOP delivered an unprecedented airborne radiometric and in situ data set related to aerosols and clouds. The Center for Interdisciplinary Remotely-Piloted Aircraft Studies (CIRPAS's) Twin Otter aircraft carried solar pointing, up- and down-looking radiometers (spectral and broadband, visible, and infrared) with the uplooking radiometers mounted on a stabilized platform. We are performing an integrated analysis of the largely unexploited radiometric data set to provide observation-based quantification of the effect of aerosols and clouds on the radiation field. We will link aerosol and cloud properties measured in situ with the observed radiative fluxes using radiative transfer models. This over-determined dataset will provide validation of the BBHRP VAP. (3) Integrated Analysis of Data from the Aerosol Lidar Validation Experiment: The ABE VAP relies on continuous lidar observations to provide the vertical distribution of the aerosols above the ARM sites. The goal of ALIVE, conducted in September 2005, was the validation of the aerosol extinction profiles obtained from the SGP Raman lidar, which has been recently refurbished/updated, and the Micro Pulse Lidar, for which a new algorithm to retrieve aerosol profiles has recently been developed, using the National Aeronautics and Space Administration (NASA) Ames Airborne Tracking 14 channel Sun photometer. We are performing and publishing the integrated analysis of the ALIVE data set.« less

Enhanced acetone-butanol-ethanol production from lignocellulosic hydrolysates by using starchy slurry as supplement.

PubMed

Yang, Ming; Kuittinen, Suvi; Vepsäläinen, Jouko; Zhang, Junhua; Pappinen, Ari

2017-11-01

This study aims to improve acetone-butanol-ethanol production from the hydrolysates of lignocellulosic material by supplementing starchy slurry as nutrients. In the fermentations of glucose, xylose and the hydrolysates of Salix schwerinii, the normal supplements such as buffer, minerals, and vitamins solutions were replaced with the barley starchy slurry. The ABE production was increased from 0.86 to 14.7g/L by supplementation of starchy slurry in the fermentation of xylose and the utilization of xylose increased from 29% to 81%. In the fermentations of hemicellulosic and enzymatic hydrolysates from S. schwerinii, the ABE yields were increased from 0 and 0.26 to 0.35 and 0.33g/g sugars, respectively. The results suggested that the starchy slurry supplied the essential nutrients for ABE fermentation. The starchy slurry as supplement could improve the ABE production from both hemicellulosic and cellulosic hydrolysate of lignocelluloses, and it is particularly helpful for enhancing the utilization of xylose from hemicelluloses. Copyright © 2017 Elsevier Ltd. All rights reserved.

Sustainable biobutanol production from pineapple waste by using Clostridium acetobutylicum B 527: Drying kinetics study.

PubMed

Khedkar, Manisha A; Nimbalkar, Pranhita R; Gaikwad, Shashank G; Chavan, Prakash V; Bankar, Sandip B

2017-02-01

Present investigation explores the use of pineapple peel, a food industry waste, for acetone-butanol-ethanol (ABE) production using Clostridium acetobutylicum B 527. Proximate analysis of pineapple peel shows that it contains 35% cellulose, 19% hemicellulose, and 16% lignin on dry basis. Drying experiments on pineapple peel waste were carried out in the temperature range of 60-120°C and experimental drying data was modeled using moisture diffusion control model to study its effect on ABE production. The production of ABE was further accomplished via acid hydrolysis, detoxification, and fermentation process. Maximum total sugar release obtained by using acid hydrolysis was 97g/L with 95-97% and 10-50% removal of phenolics and acetic acid, respectively during detoxification process. The maximum ABE titer obtained was 5.23g/L with 55.6% substrate consumption when samples dried at 120°C were used as a substrate (after detoxification). Copyright © 2016 Elsevier Ltd. All rights reserved.

Effective multiple stages continuous acetone-butanol-ethanol fermentation by immobilized bioreactors: Making full use of fresh corn stalk.

PubMed

Chang, Zhen; Cai, Di; Wang, Yong; Chen, Changjing; Fu, Chaohui; Wang, Guoqing; Qin, Peiyong; Wang, Zheng; Tan, Tianwei

2016-04-01

In order to make full use of the fresh corn stalk, the sugar containing juice was used as the sole substrate for acetone-butanol-ethanol production without any nutrients supplement, and the bagasse after squeezing the juice was used as the immobilized carrier. A total 21.34g/L of ABE was produced in batch cells immobilization system with ABE yield of 0.35g/g. A continuous fermentation containing three stages with immobilized cells was conducted and the effect of dilution rate on fermentation was investigated. As a result, the productivity and ABE solvents concentration reached 0.80g/Lh and 19.93g/L, respectively, when the dilution rate in each stage was 0.12/h (corresponding to a dilution rate of 0.04/h in the whole system). And the long-term operation indicated the continuous multiple stages ABE fermentation process had good stability and showed the great potential in future industrial applications. Copyright © 2016 Elsevier Ltd. All rights reserved.

Data supporting Al-Abed et al., Environ. Sci.: Nano, 2016,

EPA Pesticide Factsheets

Data files representing each of the Figures and Tables published in Al-Abed et al., Environ. Sci.: Nano, 2016,3, 593. The data file names identify the Figure or Table and each file contains an internal set of data definitionsThis dataset is associated with the following publication:Al-Abed, S.R., J. Virkutyte, J. Ortenzio , R.M. McCarrick, L. Degn, R. Zucker , N. Coates , K. Cleveland, H. Ma, S. Diamond, K. Dreher , and W. Boyes. Environmental aging alters AI(OH)3 coating of TiO2 nanoparticles enhancing their photocatalytic and phototoxicity activities. Environmental Science: Nano. RSC Publishing, Cambridge, UK, N/A, (2016).

Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sivagnanam, Kumaran; Raghavan, Vijaya G. S.; Shah, Manesh B

2011-01-01

Background: Butanol is a second generation biofuel produced by Clostridium acetobutylicum through acetonebutanol- ethanol (ABE) fermentation process. Shotgun proteomics provides a direct approach to study the whole proteome of an organism in depth. This paper focuses on shotgun proteomic profiling of C. acetobutylicum from ABE fermentation using glucose and xylose to understand the functional mechanisms of C. acetobutylicum proteins involved in butanol production. Results: We identified 894 different proteins in C. acetobutylicum from ABE fermentation process by two dimensional - liquid chromatography - tandem mass spectrometry (2D-LC-MS/MS) method. This includes 717 proteins from glucose and 826 proteins from the xylosemore » substrate. A total of 649 proteins were found to be common and 22 significantly differentially expressed proteins were identified between glucose and xylose substrates. Conclusion: Our results demonstrate that flagellar proteins are highly up-regulated with glucose compared to xylose substrate during ABE fermentation. Chemotactic activity was also found to be lost with the xylose substrate due to the absence of CheW and CheV proteins. This is the first report on the shotgun proteomic analysis of C. acetobutylicum ATCC 824 in ABE fermentation between glucose and xylose substrate from a single time data point and the number of proteins identified here is more than any other study performed on this organism up to this report.« less

Assessment of in situ butanol recovery by vacuum during acetone butanol ethanol (ABE) fermentation

USDA-ARS?s Scientific Manuscript database

Butanol fermentation is product limiting due to butanol toxicity to microbial cells. Butanol (boiling point: 118 deg C) boils at a greater temperature than water (boiling point: 100 deg C) and application of vacuum technology to integrated acetone-butanol-ethanol (ABE) fermentation and recovery may ...

The European AntibotABE Framework Program and Its Update: Development of Innovative Botulinum Antibodies

PubMed Central

Avril, Arnaud; Miethe, Sebastian; Derman, Yagmur; Selby, Katja; Thullier, Philippe; Pelat, Thibaut; Urbain, Remi; Korkeala, Hannu; Sesardic, Dorothea; Popoff, Michel R.

2017-01-01

The goal of the AntiBotABE Program was the development of recombinant antibodies that neutralize botulinum neurotoxins (BoNT) A, B and E. These serotypes are lethal and responsible for most human botulinum cases. To improve therapeutic efficacy, the heavy and light chains (HC and LC) of the three BoNT serotypes were targeted to achieve a synergistic effect (oligoclonal antibodies). For antibody isolation, macaques were immunized with the recombinant and non-toxic BoNT/A, B or E, HC or LC, followed by the generation of immune phage-display libraries. Antibodies were selected from these libraries against the holotoxin and further analyzed in in vitro and ex vivo assays. For each library, the best ex vivo neutralizing antibody fragments were germline-humanized and expressed as immunoglobulin G (IgGs). The IgGs were tested in vivo, in a standardized model of protection, and challenged with toxins obtained from collections of Clostridium strains. Protective antibody combinations against BoNT/A and BoNT/B were evidenced and for BoNT/E, the anti-LC antibody alone was found highly protective. The combination of these five antibodies as an oligoclonal antibody cocktail can be clinically and regulatorily developed while their high “humanness” predicts a high tolerance in humans. PMID:28974033

YTTRIUM BERYLLIDE AND COMPOUNDS OF THE TYPE ABe$sub 1$$sub 3$

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matyushenko, N.N.; Tikhinskii, G.F.

1963-11-01

Studies were made on the Be-Y system in order to establish the existence of intermetallides in it and to systematize the compounds of type ABe/sub 13/ according to their formula volumes. The existence of a cubic phase YBe/sub 13/, isomorphous with NaZn/sub 13/ and with a lattice period a = 10.238 plus or minus 0.002 A, was established. It was concluded that the known ABe/sub 13/ beryllides can be divided into three groups according to their volume relationships to the volume of the A component: Sc, Hf, Zr; Mg, Er, Y, Ca, Am; Np, U, Pu, Th, Ce, La. (P.C.H.)

Bioproduction of butanol in bioreactors: new insights from simultaneous in situ butanol recovery to eliminate product toxicity

USDA-ARS?s Scientific Manuscript database

Simultaneous acetone butanol ethanol (ABE) fermentation by Clostridium beijerinckii 260 and in situ product recovery was investigated using a vacuum process operated in two modes: continuous and intermittent. Integrated batch fermentations and ABE recovery were conducted at 37 deg C using a 14-L bio...

IQ Zoo and Teaching Operant Concepts

ERIC Educational Resources Information Center

Bihm, Elson M.; Gillaspy, J. Arthur, Jr.; Lammers, William J.; Huffman, Stephanie P.

2010-01-01

Psychology texts often cite the work of Marian and Keller Breland and their business, Animal Behavior Enterprises (ABE), to demonstrate operant conditioning and the "misbehavior of organisms" from an evolutionary perspective. Now available on the Internet at the official IQ Zoo website (http://www3.uca.edu/iqzoo/), the artifacts of ABE's work, in…

Acetone-butanol-ethanol production from Kraft paper mill sludge by simultaneous saccharification and fermentation.

PubMed

Guan, Wenjian; Shi, Suan; Tu, Maobing; Lee, Yoon Y

2016-01-01

Paper mill sludge (PS), a solid waste from pulp and paper industry, was investigated as a feedstock for acetone-butanol-ethanol (ABE) production by simultaneous saccharification and fermentation (SSF). ABE fermentation of paper sludge by Clostridium acetobutylicum required partial removal of ash in PS to enhance its enzymatic digestibility. Enzymatic hydrolysis was found to be a rate-limiting step in the SSF. A total of 16.4-18.0g/L of ABE solvents were produced in the SSF of de-ashed PS with solid loading of 6.3-7.4% and enzyme loading of 10-15FPU/g-glucan, and the final solvent yield reached 0.27g/g sugars. No pretreatment and pH control were needed in ABE fermentation of paper sludge, which makes it an attractive feedstock for butanol production. The results suggested utilization of paper sludge should not only consider the benefits of buffering effect of CaCO3 in fermentation, but also take into account its inhibitory effect on enzymatic hydrolysis. Published by Elsevier Ltd.

Efficient production of fatty acid methyl ester from waste activated bleaching earth using diesel oil as organic solvent.

PubMed

Kojima, Seiji; Du, Dongning; Sato, Masayasu; Park, Enoch Y

2004-01-01

Fatty acid methyl ester (FAME) production from waste activated bleaching earth (ABE) discarded by the crude oil refining industry was investigated using fossil fuel as a solvent in the esterification of triglycerides. Lipase from Candida cylindracea showed the highest stability in diesel oil. Using diesel oil as a solvent, 3 h was sufficient to obtain a yield of approximately 100% of FAME in the presence of 10% lipase from waste ABE. Kerosene was also a good solvent in the esterification of triglycerides embedded in the waste ABE. Fuel analysis showed that the FAME produced using diesel oil as a solvent complied with the Japanese diesel standard and the 10% residual carbon amount was lower than that of FAME produced using other solvents. Use of diesel oil as solvent in the FAME production from the waste ABE simplified the process, because there was no need to separate the organic solvent from the FAME-solvent mixture. These results demonstrate a promising reutilization method for the production of FAME, for use as a biodiesel, from industrial waste resources containing waste vegetable oils.

Linkage and Branch Analysis of High-Mannose Oligosaccharides Using Closed-Ring Labeling of 8-Aminopyrene-1,3,6-Trisulfonate and P-Aminobenzoic Ethyl Ester and Negative Ion Trap Mass Spectrometry

NASA Astrophysics Data System (ADS)

Chen, Shu-Ting; Her, Guor-Rong

2012-08-01

A strategy based on negative ion electrospray ionization tandem mass spectrometry and closed-ring labeling with both 8-aminopyrene-1,3,6-trisulfonate (APTS) and p-aminobenzoic acid ethyl ester (ABEE) was developed for linkage and branch determination of high-mannose oligosaccharides. X-type cross-ring fragment ions obtained from APTS-labeled oligosaccharides by charge remote fragmentation provided information on linkages near the non-reducing terminus. In contrast, A-type cross-ring fragment ions observed from ABEE-labeled oligosaccharides yielded information on linkages near the reducing terminus. This complementary information provided by APTS- and ABEE-labeled oligosaccharides was utilized to delineate the structures of the high-mannose oligosaccharides. As a demonstration of this approach, the linkages and branches of high-mannose oligosaccharides Man5GlcNAc2, Man6GlcNAc2, Man8GlcNAc2, and Man9GlcNAc2 cleaved from the ribonuclease B were assigned from MS2 spectra of ABEE- and APTS-labeled derivatives.

Two-stage pervaporation process for effective in situ removal acetone-butanol-ethanol from fermentation broth.

PubMed

Cai, Di; Hu, Song; Miao, Qi; Chen, Changjing; Chen, Huidong; Zhang, Changwei; Li, Ping; Qin, Peiyong; Tan, Tianwei

2017-01-01

Two-stage pervaporation for ABE recovery from fermentation broth was studied to reduce the energy cost. The permeate after the first stage in situ pervaporation system was further used as the feedstock in the second stage of pervaporation unit using the same PDMS/PVDF membrane. A total 782.5g/L of ABE (304.56g/L of acetone, 451.98g/L of butanol and 25.97g/L of ethanol) was achieved in the second stage permeate, while the overall acetone, butanol and ethanol separation factors were: 70.7-89.73, 70.48-84.74 and 9.05-13.58, respectively. Furthermore, the theoretical evaporation energy requirement for ABE separation in the consolidate fermentation, which containing two-stage pervaporation and the following distillation process, was estimated less than ∼13.2MJ/kg-butanol. The required evaporation energy was only 36.7% of the energy content of butanol. The novel two-stage pervaporation process was effective in increasing ABE production and reducing energy consumption of the solvents separation system. Copyright © 2016 Elsevier Ltd. All rights reserved.

Investigation of gas stripping and pervaporation for improved feasibility of two-stage butanol production process.

PubMed

Setlhaku, Mpho; Heitmann, Sebastian; Górak, Andrzej; Wichmann, Rolf

2013-05-01

Gas stripping and pervaporation are investigated for butanol recovery in a two-stage acetone-butanol-ethanol (ABE) fermentation process. The first stage is operated in a continuous mode and the second stage as a fed-batch. Gas stripping coupled to the second stage and operated intermittently enabled additional glucose feeding in the second stage and up to 59 g/L butanol and 73 g/L total ABE solvents in the condensate. Concentration of 167 g/L butanol and 269 g/L ABE in the permeate was measured in ex situ pervaporation experiments using a PDMS membrane at temperature of 37 °C and pressure of 10mbars. The "operating window" tool is introduced to evaluate the feasibility of the existing ABE fermentations operated as continuous with cell recycle, as two-stages, with biomass immobilization or with integrated product removal. This tool enables the identification of the most favorable process configuration, which is the combination of cell immobilization and integrated product removal. Copyright © 2013 Elsevier Ltd. All rights reserved.

Combined Detoxification and In-situ Product Removal by a Single Resin During Lignocellulosic Butanol Production

PubMed Central

Gao, Kai; Rehmann, Lars

2016-01-01

Phragmites australis (an invasive plant in North America) was used as feedstock for ABE (acetone-butanol-ethanol) fermentation by Clostridium saccharobutylicum. Sulphuric acid pretreated phragmites hydrolysate (SAEH) without detoxification inhibited butanol production (0.73 g/L butanol from 30 g/L sugars). The treatment of SAEH with resin L-493 prior the fermentation resulted in no inhibitory effects and an ABE titer of 14.44 g/L, including 5.49 g/L butanol was obtained, corresponding to an ABE yield and productivity of 0.49 g/g and 0.60 g/L/h, respectively. Dual functionality of the resin was realized by also using it as an in-situ product removal agent. Integrating in-situ product removal allowed for the use of high substrate concentrations without the typical product inhibition. Resin-detoxified SAEH was supplemented with neat glucose and an effective ABE titer of 33 g/L (including 13.7 g/L acetone, 16.4 g/L butanol and 1.9 g/L ethanol) was achieved with resin-based in-situ product removal, corresponding to an ABE yield and productivity of 0.41 g/g and 0.69 g/L/h, respectively. Both detoxification of the substrate and the products was achieved by the same resin, which was added prior the fermentation. Integrating hydrolysate detoxification and in-situ butanol removal in a batch process through single resin can potentially simplify cellulosic butanol production. PMID:27459906

Analysis of an ABE Scheme with Verifiable Outsourced Decryption.

PubMed

Liao, Yongjian; He, Yichuan; Li, Fagen; Jiang, Shaoquan; Zhou, Shijie

2018-01-10

Attribute-based encryption (ABE) is a popular cryptographic technology to protect the security of users' data in cloud computing. In order to reduce its decryption cost, outsourcing the decryption of ciphertexts is an available method, which enables users to outsource a large number of decryption operations to the cloud service provider. To guarantee the correctness of transformed ciphertexts computed by the cloud server via the outsourced decryption, it is necessary to check the correctness of the outsourced decryption to ensure security for the data of users. Recently, Li et al. proposed a full verifiability of the outsourced decryption of ABE scheme (ABE-VOD) for the authorized users and unauthorized users, which can simultaneously check the correctness of the transformed ciphertext for both them. However, in this paper we show that their ABE-VOD scheme cannot obtain the results which they had shown, such as finding out all invalid ciphertexts, and checking the correctness of the transformed ciphertext for the authorized user via checking it for the unauthorized user. We first construct some invalid ciphertexts which can pass the validity checking in the decryption algorithm. That means their "verify-then-decrypt" skill is unavailable. Next, we show that the method to check the validity of the outsourced decryption for the authorized users via checking it for the unauthorized users is not always correct. That is to say, there exist some invalid ciphertexts which can pass the validity checking for the unauthorized user, but cannot pass the validity checking for the authorized user.

Analysis of an ABE Scheme with Verifiable Outsourced Decryption

PubMed Central

He, Yichuan; Li, Fagen; Jiang, Shaoquan; Zhou, Shijie

2018-01-01

Attribute-based encryption (ABE) is a popular cryptographic technology to protect the security of users’ data in cloud computing. In order to reduce its decryption cost, outsourcing the decryption of ciphertexts is an available method, which enables users to outsource a large number of decryption operations to the cloud service provider. To guarantee the correctness of transformed ciphertexts computed by the cloud server via the outsourced decryption, it is necessary to check the correctness of the outsourced decryption to ensure security for the data of users. Recently, Li et al. proposed a full verifiability of the outsourced decryption of ABE scheme (ABE-VOD) for the authorized users and unauthorized users, which can simultaneously check the correctness of the transformed ciphertext for both them. However, in this paper we show that their ABE-VOD scheme cannot obtain the results which they had shown, such as finding out all invalid ciphertexts, and checking the correctness of the transformed ciphertext for the authorized user via checking it for the unauthorized user. We first construct some invalid ciphertexts which can pass the validity checking in the decryption algorithm. That means their “verify-then-decrypt” skill is unavailable. Next, we show that the method to check the validity of the outsourced decryption for the authorized users via checking it for the unauthorized users is not always correct. That is to say, there exist some invalid ciphertexts which can pass the validity checking for the unauthorized user, but cannot pass the validity checking for the authorized user. PMID:29320418

Combined Detoxification and In-situ Product Removal by a Single Resin During Lignocellulosic Butanol Production

NASA Astrophysics Data System (ADS)

Gao, Kai; Rehmann, Lars

2016-07-01

Phragmites australis (an invasive plant in North America) was used as feedstock for ABE (acetone-butanol-ethanol) fermentation by Clostridium saccharobutylicum. Sulphuric acid pretreated phragmites hydrolysate (SAEH) without detoxification inhibited butanol production (0.73 g/L butanol from 30 g/L sugars). The treatment of SAEH with resin L-493 prior the fermentation resulted in no inhibitory effects and an ABE titer of 14.44 g/L, including 5.49 g/L butanol was obtained, corresponding to an ABE yield and productivity of 0.49 g/g and 0.60 g/L/h, respectively. Dual functionality of the resin was realized by also using it as an in-situ product removal agent. Integrating in-situ product removal allowed for the use of high substrate concentrations without the typical product inhibition. Resin-detoxified SAEH was supplemented with neat glucose and an effective ABE titer of 33 g/L (including 13.7 g/L acetone, 16.4 g/L butanol and 1.9 g/L ethanol) was achieved with resin-based in-situ product removal, corresponding to an ABE yield and productivity of 0.41 g/g and 0.69 g/L/h, respectively. Both detoxification of the substrate and the products was achieved by the same resin, which was added prior the fermentation. Integrating hydrolysate detoxification and in-situ butanol removal in a batch process through single resin can potentially simplify cellulosic butanol production.

The AstroBiology Explorer (ABE) MIDEX Mission Concept: Using Infrared Spectroscopy to Identify Organic Molecules in Space

NASA Technical Reports Server (NTRS)

Sandford, Scott A.; Vincenzi, Donald (Technical Monitor)

2002-01-01

One of the principal means by which organic compounds are detected and identified in space is by infrared spectroscopy. Past IR studies (telescopic and laboratory) have demonstrated that much of the carbon in the interstellar medium (ISM) is in complex organic species of a variety of types, but the distribution, abundance, and evolutionary relationships of these materials are not well understood. The Astrobiology Explorer (ABE) is a MIDEAST mission concept designed to conduct IR spectroscopic observations to detect and identify these materials to address outstanding important problems in astrobiology, astrochemistry, and astrophysics. Systematic studies include the observation of planetary nebulae and stellar outflows, protostellar objects, Solar System Objects, and galaxies, and multiple lines of sight through dense molecular clouds and the diffuse ISM. ABE will also search for evidence of D enrichment in complex molecules in all these environments. The mission is currently under study at NASA's Ames Research Center in collaboration with Ball Aerospace and Technologies Corp. ABE is a cryogenically-cooled 60 cm diameter space telescope equipped with 3 cryogenic cross-dispersed spectrographs that share a single common slit. The 3 spectrometers each measure single spectral octaves (2.5-5, 5-10, 10-20 microns) and together cover the entire 2.5 - 20 micron region simultaneously. The spectrometers use state-of-the-art 1024x1024 pixel detectors, with a single InSb array for the 2.5-5 micron region and two Si:As arrays for the 5-10 and 10-20 micron regions. The spectral resolution is wavelength dependent but is greater than 2000 across the entire spectral range. ABE would operate in a heliocentric, Earth drift-away orbit and is designed to take maximum advantage of this environment for cooling, thermal stability, and mission lifetime. ABE would have a core science mission lasting approximately 1.5 years.

Proceedings of the Expert Systems Workshop Held in Pacific Grove, California on 16-18 April 1986

DTIC Science & Technology

1986-04-18

13- NUMBER OF PAGES 197 N IS. SECURITY CLASS, (ol Mm raport) UNCLASSIFIED I5a. DECLASSIFI CATION/DOWNGRADING SCHEDULE 16. DISTRIBUTION...are distributed and parallel. * - Features unimplemented at present; scheduled for phase 2. Table 1-1: Key design characteristics of ABE 2. a...data structuring techniques and a semi- deterministic scheduler . A program for the DF framework consists of a number of independent processing modules

Aviation: Boatswain's Mate E 1 and C; Rate Training Manual.

ERIC Educational Resources Information Center

Naval Education and Training Command, Pensacola, FL.

The rate training manual has been prepared for enlisted personnel of the Navy and Naval Reserve who are studying for advancement in the Aviation Boatswain's Mate E rating. It is primarily based on the professional requirements or qualifications for ABE 1 and ABE C, as contained in the Manual of Qualifications for Advancement NavPers 18068…

Microbial production of a biofuel (acetone-butanol-ethanol) in a continuous bioreactor: impact of bleed and simultaneous product removal

USDA-ARS?s Scientific Manuscript database

Acetone butanol ethanol (ABE) was produced in an integrated continuous fermentation and product recovery system using a microbial strain Clostridium beijerinckii BA101 for ABE production and fermentation gases (CO2 and H2) for product removal by gas stripping. This represents a continuation of our ...

What Are the Security Requirements for a Two-State Solution between Israel and Palestine?

DTIC Science & Technology

2011-03-01

Economic Viability of a Palestinian State...no. 2 (Spring, 1997), 215–229, http://www.jstor.org/stable/4329054. 103 Abed, The Economic Viability of a Palestinian State, 9. 58 might have...LIST OF REFERENCES Abed, George T. The Economic Viability of a Palestinian State. (Washington D.C.: Institute for Palestine Studies, 1990).

The Attentional Boost Effect and Context Memory

ERIC Educational Resources Information Center

Mulligan, Neil W.; Smith, S. Adam; Spataro, Pietro

2016-01-01

Stimuli co-occurring with targets in a detection task are better remembered than stimuli co-occurring with distractors--the attentional boost effect (ABE). The ABE is of interest because it is an exception to the usual finding that divided attention during encoding impairs memory. The effect has been demonstrated in tests of item memory but it is…

Metal separations using aqueous biphasic partitioning systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chaiko, D.J.; Zaslavsky, B.; Rollins, A.N.

1996-05-01

Aqueous biphasic extraction (ABE) processes offer the potential for low-cost, highly selective separations. This countercurrent extraction technique involves selective partitioning of either dissolved solutes or ultrafine particulates between two immiscible aqueous phases. The extraction systems that the authors have studied are generated by combining an aqueous salt solution with an aqueous polymer solution. They have examined a wide range of applications for ABE, including the treatment of solid and liquid nuclear wastes, decontamination of soils, and processing of mineral ores. They have also conducted fundamental studies of solution microstructure using small angle neutron scattering (SANS). In this report they reviewmore » the physicochemical fundamentals of aqueous biphase formation and discuss the development and scaleup of ABE processes for environmental remediation.« less

Continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using Clostridium beijerinckii BA101.

PubMed

Ezeji, T C; Qureshi, N; Blaschek, H P

2005-01-26

Use of starch solution as feed for butanol bioconversion processes employing Clostridium beijerinckii BA101 may have added economic advantage over the use of glucose. Acetone butanol ethanol (ABE) was produced from 30 gL(-1) starch solution using a continuous process. The bioreactor was fed at a dilution rate of 0.02 h(-1) and starch solution/feed volume (3 L) was replaced every 72 h. The continuous reactor fed with cornstarch solution (feed temperature 19 degrees C) produced approximately 6.0 gL(-1) total ABE. Increasing the feed storage temperature to 37 degrees C improved ABE production to 7.2 gL(-1) suggesting that retrogradation was occurring more rapidly at 19 degrees C. In both these cases the fermentation drifted toward acid production after approximately 260 h, consistent with the retrogradation of starch overtime. The use of soluble starch, which is less prone to retrogradation, resulted in the production of 9.9 gL(-1) ABE at 37 degrees C feed storage temperature, as compared to 7.2 gL(-1) ABE when cornstarch was used. It should be noted that gelatinized starch retrogradation takes place after sterilization and prior to use of the feed medium, and does not occur during long-term storage of the raw corn material in the months leading up to processing. The degree of hydrolysis of gelatinized starch decreased from 68.8 to 56.2% in 3 days when stored at 37 degrees C. Soluble starch which does not retrograde demonstrated no change in the degree of hydrolysis.

The attentional boost effect and context memory.

PubMed

Mulligan, Neil W; Smith, S Adam; Spataro, Pietro

2016-04-01

Stimuli co-occurring with targets in a detection task are better remembered than stimuli co-occurring with distractors-the attentional boost effect (ABE). The ABE is of interest because it is an exception to the usual finding that divided attention during encoding impairs memory. The effect has been demonstrated in tests of item memory but it is unclear if context memory is likewise affected. Some accounts suggest enhanced perceptual encoding or associative binding, predicting an ABE on context memory, whereas other evidence suggests a more abstract, amodal basis of the effect. In Experiment 1, context memory was assessed in terms of an intramodal perceptual detail, the font and color of the study word. Experiment 2 examined context memory cross-modally, assessing memory for the modality (visual or auditory) of the study word. Experiments 3 and 4 assessed context memory with list discrimination, in which 2 study lists are presented and participants must later remember which list (if either) a test word came from. In all experiments, item (recognition) memory was also assessed and consistently displayed a robust ABE. In contrast, the attentional-boost manipulation did not enhance context memory, whether defined in terms of visual details, study modality, or list membership. There was some evidence that the mode of responding on the detection task (motoric response as opposed to covert counting of targets) may impact context memory but there was no evidence of an effect of target detection, per se. In sum, the ABE did not occur in context memory with verbal materials. (c) 2016 APA, all rights reserved).

Production of Butanol (a Biofuel) from Agricultural Residues: Part II - Use of Corn Stover and Switchgrass Hydrolysates

USDA-ARS?s Scientific Manuscript database

Acetone butanol ethanol (ABE or AB, or solvent) was produced from hydrolyzed corn stover and switchgrass using Clostridium beijerinckii P260. A control experiment using glucose resulted in the production of 21.06 gL**-1 total ABE. In this experiment, an AB yield and productivity of 0.41 and 0.31 g...

Toward Privacy-preserving Content Access Control for Information Centric Networking

DTIC Science & Technology

2014-03-01

REPORT Toward Privacy-preserving Content Access Control for Information Centric Networking 14. ABSTRACT 16. SECURITY CLASSIFICATION OF: Information...regardless the security mechanisms provided by different content hosting servers. However, using ABE has a drawback that the enforced content access...Encryption (ABE) is a flexible approach to enforce the content access policies regardless the security mechanisms provided by different content hosting

CaCO3 supplementation alleviates the inhibition of formic acid on acetone/butanol/ethanol fermentation by Clostridium acetobutylicum.

PubMed

Qi, Gaoxiang; Xiong, Lian; Lin, Xiaoqing; Huang, Chao; Li, Hailong; Chen, Xuefang; Chen, Xinde

2017-01-01

To investigate the inhibiting effect of formic acid on acetone/butanol/ethanol (ABE) fermentation and explain the mechanism of the alleviation in the inhibiting effect under CaCO 3 supplementation condition. From the medium containing 50 g sugars l -1 and 0.5 g formic acid l -1 , only 0.75 g ABE l -1 was produced when pH was adjusted by KOH and fermentation ended prematurely before the transformation from acidogenesis to solventogenesis. In contrast, 11.4 g ABE l -1 was produced when pH was adjusted by 4 g CaCO 3 l -1 . The beneficial effect can be ascribed to the buffering capacity of CaCO 3 . Comparative analysis results showed that the undissociated formic acid concentration and acid production coupled with ATP and NADH was affected by the pH buffering capacity of CaCO 3 . Four millimole undissociated formic acid was the threshold at which the transformation to solventogenesis occurred. The inhibiting effect of formic acid on ABE fermentation can be alleviated by CaCO 3 supplementation due to its buffering capacity.

Shotgun proteomic monitoring of Clostridium acetobutylicum during stationary phase of butanol fermentation using xylose and comparison with the exponential phase

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sivagnanam, Kumaran; Raghavan, Vijaya G. S.; Shah, Manesh B

2012-01-01

Economically viable production of solvents through acetone butanol ethanol (ABE) fermentation requires a detailed understanding of Clostridium acetobutylicum. This study focuses on the proteomic profiling of C. acetobutylicum ATCC 824 from the stationary phase of ABE fermentation using xylose and compares with the exponential growth by shotgun proteomics approach. Comparative proteomic analysis revealed 22.9% of the C. acetobutylicum genome and 18.6% was found to be common in both exponential and stationary phases. The proteomic profile of C. acetobutylicum changed during the ABE fermentation such that 17 proteins were significantly differentially expressed between the two phases. Specifically, the expression of fivemore » proteins namely, CAC2873, CAP0164, CAP0165, CAC3298, and CAC1742 involved in the solvent production pathway were found to be significantly lower in the stationary phase compared to the exponential growth. Similarly, the expression of fucose isomerase (CAC2610), xylulose kinase (CAC2612), and a putative uncharacterized protein (CAC2611) involved in the xylose utilization pathway were also significantly lower in the stationary phase. These findings provide an insight into the metabolic behavior of C. acetobutylicum between different phases of ABE fermentation using xylose.« less

Conversion of food processing wastes to biofuel using clostridia.

PubMed

Abd-Alla, Mohamed Hemida; Zohri, Abdel-Naser Ahmed; El-Enany, Abdel-Wahab Elsadek; Ali, Shimaa Mohamed

2017-12-01

This study aims to demonstrate the recycling of food processing wastes as a low cost-effective substrate for acetone - butanol - ethanol (ABE) production. Potato peels and cheese whey were utilized during fermentation with eight local Clostridium strains in addition to the commercial strain, C. acetobutylicum ATCC 824 for ABE and organic acids production. From potato peels, Clostridium beijerinckii ASU10 produced the highest ABE production (17.91 g/l) representing 61.3% butanol (10.98 g/l), 33.6% acetone (6.02 g/l) and 5.1% ethanol (0.91 g/l). While, C. chauvoei ASU12 showed the highest acid production (8.15 g/l) including 5.50 and 2.61 g/l acetic and butyric acids, respectively. Use of cheese whey as fermentable substrate exhibited a substantial increase in ethanol ratio and decrease in butanol ratio compared to those produced from potato peels. Clostridium beijerinckii ASU5 produced the highest ABE concentration (7.13 g/l) representing 50.91% butanol (3.63 g/l), 35.34% acetone (2.52 g/l) and 13.74% ethanol (0.98 g/l). The highest acid production (8.00 g/l) was obtained by C. beijerinckii ASU5 representing 4.89 and 3.11 g/l for acetic and butyric acid, respectively. Supplementation of potato peels with an organic nitrogen source showed NH 4 NO 3 promoted ABE production more than yeast extract. In conclusion, this study introduced an ecofriendly and economical practice for utilization of food processing wastes (renewable substrates as potato peels and cheese whey) for biofuel production using various Clostridium strains. Copyright © 2017 Elsevier Ltd. All rights reserved.

The advanced strategy for enhancing biobutanol production and high-efficient product recovery with reduced wastewater generation.

PubMed

Xue, Chuang; Zhang, Xiaotong; Wang, Jufang; Xiao, Min; Chen, Lijie; Bai, Fengwu

2017-01-01

Butanol as an important chemical and potential fuel could be produced via ABE fermentation from lignocellulosic biomass. The use of food-related feedstocks such as maize and sugar cane may not be a sustainable solution to world's energy needs. Recently, Jerusalem artichoke tubers containing inulin have been used as feedstock for butanol production, but this bioprocess is not commercially feasible due to the great value of inulin as functional food. Till now, there is a gap on the utilization of Jerusalem artichoke stalk (JAS) as feedstock for microbial butanol production. Biobutanol production from JAS was investigated in order to improve cellulose digestibility and efficient biobutanol fermentation. Compared with 9.0 g/L butanol (14.7 g/L ABE) production by 2% NaOH pretreatment of JAS, 11.8 g/L butanol (17.6 g/L ABE) was produced in the best scenario conditions of NaOH-H 2 O 2 pretreatment, washing times and citrate buffer strengths etc. Furthermore, more than >64% water in washing pretreated JAS process could be saved, with improving butanol production by >25.0%. To mimic in situ product recovery for ABE fermentation, the vapor stripping-vapor permeation (VSVP) process steadily produced 323.4-348.7 g/L butanol (542.7-594.0 g/L ABE) in condensate, which showed more potentials than pervaporation for butanol recovery. Therefore, the present study demonstrated an effective strategy on efficient biobutanol production using lignocellulosic biomass. The process optimization could contribute to significant reduction of wastewater emission and the improvement of lignocellulosic biomass digestibility and biobutanol production, which makes biobutanol production more efficient using JAS.

NH4+ transport system of a psychrophilic marine bacterium, Vibrio sp. strain ABE-1.

PubMed

Chou, M; Matsunaga, T; Takada, Y; Fukunaga, N

1999-05-01

NH4(+) transport system of a psychrophilic marine bacterium Vibrio sp. strain ABE-1 (Vibrio ABE-1) was examined by measuring the uptake of [14C]methylammonium ion (14CH3NH3+) into the intact cells. 14CH3NH3+ uptake was detected in cells grown in medium containing glutamate as the sole nitrogen source, but not in those grown in medium containing NH4Cl instead of glutamate. Vibrio ABE-1 did not utilize CH3NH3+ as a carbon or nitrogen source. NH4Cl and nonradiolabeled CH3NH3+ completely inhibited 14CH3NH3+ uptake. These results indicate that 14CH3NH3+ uptake in this bacterium is mediated via an NH4+ transport system and not by a specific carrier for CH3NH3+. The respiratory substrate succinate was required to drive 14CH3NH3+ uptake and the uptake was completely inhibited by KCN, indicating that the uptake was energy dependent. The electrochemical potentials of H+ and/or Na+ across membranes were suggested to be the driving forces for the transport system because the ionophores carbonylcyanide m-chlorophenylhydrazone and monensin strongly inhibited uptake activities at pH 6.5 and 8.5, respectively. Furthermore, KCl activated 14CH3NH3+ uptake. The 14CH3NH3+ uptake activity of Vibrio ABE-1 was markedly high at temperatures between 0 degrees and 15 degrees C, and the apparent Km value for CH3NH3+ of the uptake did not change significantly over the temperature range from 0 degrees to 25 degrees C. Thus, the NH4+ transport system of this bacterium was highly active at low temperatures.

Autonomous Buoyed Environmental Measurement System (ABES)

DTIC Science & Technology

1997-09-30

GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND... ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S) 11. SPONSOR/MONITOR’S REPORT NUMBER(S...azimuth and dip of the Earth’s magnetic field. It is a 3-V device, available in 16-pin small outline integrated circuit ( SOIC ) form factor. Cost is $4

Mineralogy and petrology of the Abee enstatite chondrite breccia and its dark inclusions

NASA Technical Reports Server (NTRS)

Rubin, A. E.; Keil, K.

1983-01-01

A model is proposed for the petrogenesis of the Abee E4 enstatite chondrite breccia, which consists of clasts, dark inclusions and matrix, and whose dark inclusions are an unusual kind of enstatite chondritic material. When the maximum metamorphic temperature of the breccia parent material was greater than 840 C, euhedral enstatite crystals in metallic Fe, Ni, and sulfide-rich areas grew into pliable metal and sulfide. Breccia parent material was impact-excavated, admixed with dark inclusions, and rapidly cooled. During this cooling, the clast and matrix material acquired thermal remanent magnetization. A subsequent ambient magnetic field imparted a uniform net magnetic orientation to the matrix and caused the magnetic orientation of the clasts to be less random. The Abee breccia was later consolidated by shock or by shallow burial and long period, low temperature metamorphism.

Decadal changes in CH4 and CO2 emissions on the Alaskan North Slope

NASA Astrophysics Data System (ADS)

Sweeney, C.; Commane, R.; Wofsy, S.; Dlugokencky, E. J.; Karion, A.; Stone, R. S.; Chang, R.; Tans, P. P.; Wolter, S.

2016-12-01

Large changes in surface air temperature, sea ice cover and permafrost in the Arctic Boreal Ecosystems (ABE) are significantly impacting the critical ecosystem services and human societies that are dependent on the ABE. In order to predict the outcome of continued change in the climate system of the ABE, it is necessary to look at how past changes in climate have affected the ABE. We look at 30 years of CH4 and 42 years of CO2 observations from the NOAA Global Greenhouse Gas Reference Network site in Barrow, Alaska. By eliminating background trends and only looking at data collected when winds are blowing off the North Slope we find very little change in CH4 enhancements, but significant changes in the CO2 enhancements coming off the tundra. The bulk of both CO2 and CH4 emissions appear to be emitted well after the first snow fall on the North Slope. CO2 emissions are a strongly correlation with summer surface temperatures, while CH4 emissions appear insensitive to the large temperature changes that occurred over the measurement period. These results suggest that CO2, and not CH4 emissions, are a likely pathway for the degradation of permafrost carbon.

An approach for sample size determination of average bioequivalence based on interval estimation.

PubMed

Chiang, Chieh; Hsiao, Chin-Fu

2017-03-30

In 1992, the US Food and Drug Administration declared that two drugs demonstrate average bioequivalence (ABE) if the log-transformed mean difference of pharmacokinetic responses lies in (-0.223, 0.223). The most widely used approach for assessing ABE is the two one-sided tests procedure. More specifically, ABE is concluded when a 100(1 - 2α) % confidence interval for mean difference falls within (-0.223, 0.223). As known, bioequivalent studies are usually conducted by crossover design. However, in the case that the half-life of a drug is long, a parallel design for the bioequivalent study may be preferred. In this study, a two-sided interval estimation - such as Satterthwaite's, Cochran-Cox's, or Howe's approximations - is used for assessing parallel ABE. We show that the asymptotic joint distribution of the lower and upper confidence limits is bivariate normal, and thus the sample size can be calculated based on the asymptotic power so that the confidence interval falls within (-0.223, 0.223). Simulation studies also show that the proposed method achieves sufficient empirical power. A real example is provided to illustrate the proposed method. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

Mathematical modelling of clostridial acetone-butanol-ethanol fermentation.

PubMed

Millat, Thomas; Winzer, Klaus

2017-03-01

Clostridial acetone-butanol-ethanol (ABE) fermentation features a remarkable shift in the cellular metabolic activity from acid formation, acidogenesis, to the production of industrial-relevant solvents, solventogensis. In recent decades, mathematical models have been employed to elucidate the complex interlinked regulation and conditions that determine these two distinct metabolic states and govern the transition between them. In this review, we discuss these models with a focus on the mechanisms controlling intra- and extracellular changes between acidogenesis and solventogenesis. In particular, we critically evaluate underlying model assumptions and predictions in the light of current experimental knowledge. Towards this end, we briefly introduce key ideas and assumptions applied in the discussed modelling approaches, but waive a comprehensive mathematical presentation. We distinguish between structural and dynamical models, which will be discussed in their chronological order to illustrate how new biological information facilitates the 'evolution' of mathematical models. Mathematical models and their analysis have significantly contributed to our knowledge of ABE fermentation and the underlying regulatory network which spans all levels of biological organization. However, the ties between the different levels of cellular regulation are not well understood. Furthermore, contradictory experimental and theoretical results challenge our current notion of ABE metabolic network structure. Thus, clostridial ABE fermentation still poses theoretical as well as experimental challenges which are best approached in close collaboration between modellers and experimentalists.

Biocatalyzed processes for production of commodity chemicals: Assessment of future research advances for N-butanol production

NASA Technical Reports Server (NTRS)

Ingham, J. D.

1984-01-01

This report is a summary of assessments by Chem Systems Inc. and a further evaluation of the impacts of research advances on energy efficiency and the potential for future industrial production of acetone-butanol-ethanol (ABE) solvents and other products by biocatalyzed processes. Brief discussions of each of the assessments made by CSI, followed by estimates of minimum projected energy consumption and costs for production of solvents by ABE biocatalyzed processes are included. These assessments and further advances discussed in this report show that substantial decreases in energy consumption and costs are possible on the basis of specific research advances; therefore, it appears that a biocatalyzed process for ABE can be developed that will be competitive with conventional petrochemical processes for production of n-butanol and acetone. (In this work, the ABE process was selected and utilized only as an example for methodology development; other possible bioprocesses for production of commodity chemicals are not intended to be excluded.) It has been estimated that process energy consumption can be decreased by 50%, with a corresponding cost reduction of 15-30% (in comparison with a conventional petrochemical process) by increasing microorganism tolerance to n-butanol and efficient recovery of product solvents from the vapor phase.

Software-Defined Radio Global System for Mobile Communications Transmitter Development for Heterogeneous Network Vulnerability Testing

DTIC Science & Technology

2013-12-01

AbdelWahab, “ 2G / 3G Inter-RAT Handover Performance Analysis,” Second European Conference on Antennas and Propagation, pp. 1, 8, 11–16, Nov. 2007. [19] J...RADIO GLOBAL SYSTEM FOR MOBILE COMMUNICATIONS TRANSMITTER DEVELOPMENT FOR HETEROGENEOUS NETWORK VULNERABILITY TESTING by Carson C. McAbee... MOBILE COMMUNICATIONS TRANSMITTER DEVELOPMENT FOR HETEROGENEOUS NETWORK VULNERABILITY TESTING 5. FUNDING NUMBERS 6. AUTHOR(S) Carson C. McAbee

Bio-plasticizer production by hybrid acetone-butanol-ethanol fermentation with full cell catalysis of Candida sp. 99-125.

PubMed

Chen, Changjing; Cai, Di; Qin, Peiyong; Chen, Biqiang; Wang, Zheng; Tan, Tianwei

2018-06-01

Hybrid process that integrated fermentation, pervaporation and esterification was established aiming to improve the economic feasibility of the conventional acetone-butanol-ethanol (ABE) fermentation process. Candida sp 99-125 cells were used as full-cell catalyst. The feasibility of batch and fed-batch esterification using the ABE permeate of pervaporation (ranging from 286.9 g/L to 402.9 g/L) as substrate were compared. Valuable butyl oleate was produced along with ethyl oleate. For the batch esterification, due to severe inhibition of substrate to lipase, the yield of butyl oleate and ethyl oleate were only 24.9% and 3.3%, respectively. In contrast, 75% and 11.8% of butyl oleate and ethyl oleate were obtained, respectively, at the end of the fed-batch esterification. The novel integration process provides a promising strategy for in situ upgrading ABE products. Copyright © 2018 Elsevier Ltd. All rights reserved.

Production of acetone, butanol, and ethanol from biomass of the green seaweed Ulva lactuca.

PubMed

van der Wal, Hetty; Sperber, Bram L H M; Houweling-Tan, Bwee; Bakker, Robert R C; Brandenburg, Willem; López-Contreras, Ana M

2013-01-01

Green seaweed Ulva lactuca harvested from the North Sea near Zeeland (The Netherlands) was characterized as feedstock for acetone, ethanol and ethanol fermentation. Solubilization of over 90% of sugars was achieved by hot-water treatment followed by hydrolysis using commercial cellulases. A hydrolysate was used for the production of acetone, butanol and ethanol (ABE) by Clostridium acetobutylicum and Clostridium beijerinckii. Hydrolysate-based media were fermentable without nutrient supplementation. C. beijerinckii utilized all sugars in the hydrolysate and produced ABE at high yields (0.35 g ABE/g sugar consumed), while C. acetobutylicum produced mostly organic acids (acetic and butyric acids). These results demonstrate the great potential of U. lactuca as feedstock for fermentation. Interestingly, in control cultures of C. beijerinckii on rhamnose and glucose, 1,2 propanediol was the main fermentation product (9.7 g/L). Copyright © 2012 Elsevier Ltd. All rights reserved.

Is downer cow syndrome related to chronic botulism?

PubMed

Rulff, R; Schrödl, W; Basiouni, S; Neuhaus, J; Krüger, M

2015-01-01

The present work was directed to investigate the relationship between Downer cow syndrome (DCS) and chronic botulism in dairy cattle. For this purpose, a total of 52 fresh calving downer cows and 206 apparently healthy cows at 14 dairy farms were investigated for Clostridium botulinum ABE and CD antibody levels, C. botulinum and botulinum neurotoxin in rumen fluids as well as in faeces. Results indicated that the downer cows had higher IgG titers for C. botulinum ABE and CD than the healthy cows. All tested rumen fluids were negative for BoNT and C. botulinum. BoNT/D, however, and C. botulinum type D spores were detected in faecal samples of healthy and downer cows in the selected farms. In conclusion, the presence of a significantly higher C. botulinum ABE and CD antibody levels in DCS cows than in the healthy animals suggests that chronic C. botulinum toxico-infection could be a predisposing factor for DCS.

System-level modeling of acetone-butanol-ethanol fermentation.

PubMed

Liao, Chen; Seo, Seung-Oh; Lu, Ting

2016-05-01

Acetone-butanol-ethanol (ABE) fermentation is a metabolic process of clostridia that produces bio-based solvents including butanol. It is enabled by an underlying metabolic reaction network and modulated by cellular gene regulation and environmental cues. Mathematical modeling has served as a valuable strategy to facilitate the understanding, characterization and optimization of this process. In this review, we highlight recent advances in system-level, quantitative modeling of ABE fermentation. We begin with an overview of integrative processes underlying the fermentation. Next we survey modeling efforts including early simple models, models with a systematic metabolic description, and those incorporating metabolism through simple gene regulation. Particular focus is given to a recent system-level model that integrates the metabolic reactions, gene regulation and environmental cues. We conclude by discussing the remaining challenges and future directions towards predictive understanding of ABE fermentation. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Co-generation of microbial lipid and bio-butanol from corn cob bagasse in an environmentally friendly biorefinery process.

PubMed

Cai, Di; Dong, Zhongshi; Wang, Yong; Chen, Changjing; Li, Ping; Qin, Peiyong; Wang, Zheng; Tan, Tianwei

2016-09-01

Biorefinery process of corn cob bagasse was investigated by integrating microbial lipid and ABE fermentation. The effects of NaOH concentration on the fermentations performance were evaluated. The black liquor after pretreatment was used as substrate for microbial lipid fermentation, while the enzymatic hydrolysates of the bagasse were used for ABE fermentation. The results demonstrated that under the optimized condition, the cellulose and hemicellulose in raw material could be effectively utilized. Approximate 87.7% of the polysaccharides were converted into valuable biobased products (∼175.7g/kg of ABE along with ∼36.6g/kg of lipid). At the same time, almost half of the initial COD (∼48.9%) in the black liquor could be degraded. The environmentally friendly biorefinery process showed promising in maximizing the utilization of biomass for future biofuels production. Copyright © 2016 Elsevier Ltd. All rights reserved.

A Parallel Hypothesis Method of Autonomous Underwater Vehicle Navigation

DTIC Science & Technology

2009-06-01

that is a hybrid of instantaneous and filtered localization methods. As discussed in Smith’s text on mathematical modeling and digital simulation ...rich in acoustic multipaths. The method was extended, in simulation , to other shallow water environments which could also be expected to be rich in...and the observed water depth profile is shown in Figure 6-17. The ABE164 survey dive ended early because ABE became entangled in a piece of 1/4

India-U.S. Relations

DTIC Science & Technology

2009-01-30

Trident—along with the main railway terminal, a Jewish cultural center, a café frequented by foreigners, a cinema house, and two hospitals. Six...seats. A mid-2007 visit to New Delhi by Japanese Prime Minister Shinzo Abe, who was effusive in his praise of India as a “partner and friend,” was...seen by many as part of a long- term Japanese effort to hedge against China’s growing regional influence. Abe and Prime Minister Singh issued a

Lipase-catalyzed biodiesel production from waste activated bleaching earth as raw material in a pilot plant.

PubMed

Park, Enoch Y; Sato, Masayasu; Kojima, Seiji

2008-05-01

The production of fatty acid methyl esters (FAMEs) from waste activated bleaching earth (ABE) discarded by the crude oil refining industry using lipase from Candida cylindracea was investigated in a 50-L pilot plant. Diesel oil or kerosene was used as an organic solvent for the transesterification of triglycerides embedded in the waste ABE. When 1% (w/w) lipase was added to waste ABE, the FAME content reached 97% (w/w) after reaction for 12 h at 25 degrees C with an agitation rate of 30 rpm. The FAME production rate was strongly dependent upon the amount of enzyme added. Mixtures of FAME and diesel oil at ratios of 45:55 (BDF-45) and 35:65 (BDF-35) were assessed and compared with the European specifications for biodiesel as automotive diesel fuel, as defined by pr EN 14214. The biodiesel quality of BDF-45 met the EN 14214 standard. BDF-45 was used as generator fuel, and the exhaust emissions were compared with those of diesel oil. The CO and SO2 contents were reduced, but nitrogen oxide emission increased by 10%. This is the first report of a pilot plant study of lipase-catalyzed FAME production using waste ABE as a raw material. This result demonstrates a promising reutilization method for the production of FAME from industrial waste resources containing vegetable oils for use as a biodiesel fuel.

Glycerol supplementation of the growth medium enhances in situ detoxification of furfural by Clostridium beijerinckii during butanol fermentation.

PubMed

Ujor, Victor; Agu, Chidozie Victor; Gopalan, Venkat; Ezeji, Thaddeus Chukwuemeka

2014-01-01

Lignocellulose-derived microbial inhibitors such as furfural and 5-hydroxymethyl furfural adversely affect fermentation of lignocellulosic biomass hydrolysates to fuels and chemicals due to their toxicity on fermenting microbes. To harness the potential of lignocellulose as a cheap source of fermentable sugars, in situ detoxification of furfural and other lignocellulose-derived microbial inhibitors is essential. To enhance in situ detoxification and tolerance of furfural by Clostridium beijerinckii NCIMB 8052 during acetone-butanol-ethanol (ABE) fermentation, the effect of glycerol on NADH/NADPH generation and ABE production by furfural (4, 5, and 6 g/L)-challenged cultures was investigated in this study. In all instances, beneficial outcomes were observed. For example, the fermentation medium supplemented with glycerol and subjected to 5 g/L furfural elicited up to 1.8- and 3-fold increases, respectively, in NADH and NADPH levels in C. beijerinckii 8052 relative to the control culture. These critical changes are the likely underpinnings for the glycerol-mediated 2.3-fold increase in the rate of detoxification of 5 g/L furfural, substrate consumption, and ABE production compared to the unsupplemented medium. Collectively, these results demonstrate that increased intracellular NADH/NADPH in C. beijerinckii 8052 due to glycerol utilization engenders favorable effects on many aspects of cellular metabolism, including enhanced furfural reduction and increased ABE production.

A dynamic metabolic flux analysis of ABE (acetone-butanol-ethanol) fermentation by Clostridium acetobutylicum ATCC 824, with riboflavin as a by-product.

PubMed

Zhao, Xinhe; Kasbi, Mayssa; Chen, Jingkui; Peres, Sabine; Jolicoeur, Mario

2017-12-01

The present study reveals that supplementing sodium acetate (NaAc) strongly stimulates riboflavin production in acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum ATCC 824 with xylose as carbon source. Riboflavin production increased from undetectable concentrations to ∼0.2 g L -1 (0.53 mM) when supplementing 60 mM NaAc. Of interest, solvents production and biomass yield were also promoted with fivefold acetone, 2.6-fold butanol, and 2.4-fold biomass adding NaAc. A kinetic metabolic model, developed to simulate ABE biosystem, with riboflavin production, revealed from a dynamic metabolic flux analysis (dMFA) simultaneous increase of riboflavin (ribA) and GTP (precursor of riboflavin) (PurM) synthesis flux rates under NaAc supplementation. The model includes 23 fluxes, 24 metabolites, and 72 kinetic parameters. It also suggested that NaAc condition has first stimulated the accumulation of intracellular metabolite intermediates during the acidogenic phase, which have then fed the solventogenic phase leading to increased ABE production. In addition, NaAc resulted in higher intracellular levels of NADH during the whole culture. Moreover, lower GTP-to-adenosine phosphates (ATP, ADP, AMP) ratio under NaAc supplemented condition suggests that GTP may have a minor role in the cell energetic metabolism compared to its contribution to riboflavin synthesis. © 2017 Wiley Periodicals, Inc.

Scaling Projections for Sb-based p-channel FETs

DTIC Science & Technology

2010-01-01

the products of long-standing programs on antimonide growth by molecular beam epitaxy at the QinetiQ Corp. (for InSb) and at the Naval Research...electron mobilities in the channels of III–V HEMTs at room temperature are much higher than in Si or Ge, e.g., in InAs they are in the range of 20–30,000 cm2... HEMT structures. IEEE Trans Electron Dev 1985;32:11. [25] Awano Y, Kosugi M, Kosemura K, Mimura T, Abe M. Short-channel effects in subquarter

Particle simulation of Coulomb collisions: Comparing the methods of Takizuka and Abe and Nanbu

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang Chiaming; Lin, Tungyou; Caflisch, Russel

2008-04-20

The interactions of charged particles in a plasma are governed by long-range Coulomb collision. We compare two widely used Monte Carlo models for Coulomb collisions. One was developed by Takizuka and Abe in 1977, the other was developed by Nanbu in 1997. We perform deterministic and statistical error analysis with respect to particle number and time step. The two models produce similar stochastic errors, but Nanbu's model gives smaller time step errors. Error comparisons between these two methods are presented.

Improved efficiency of butanol production by absorbed lignocellulose fermentation.

PubMed

He, Qin; Chen, Hongzhang

2013-03-01

Alkali-treated steam-exploded corn stover (SECSAT) was used as solid substrate for acetone-butanol-ethanol (ABE) production by absorbed lignocellulose fermentation (ALF) using Clostridium acetobutylicum ATCC 824. The ABE concentration in ALF culture had increased by 47% compared with that in submerged culture. More surprisingly, the acetone production was promoted and ethanol production was lower in the presence of SECSAT than that in its absence. ALF was also successfully in cofermentation of glucose and xylose, although decreased fermentability with an increase in the proportion of xylose. An invariable chemical composition and dry weight of SECSAT was found in ALF. Partial simultaneous saccharification and fermentation of SECSAT using a certain amount of cellulase could not only enhance the ABE concentration by 71%, but also significantly increase the area proportion of fiber cells in SECSAT from 53% to 90%, which would be an excellent paper making material. Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Butanol production from wood pulping hydrolysate in an integrated fermentation-gas stripping process

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lu, CC; Dong, J; Yang, ST

2013-09-01

Wood pulping hydrolysate (WPH) containing mainly xylose and glucose as a potential substrate for acetone-butanol-ethanol (ABE) fermentation was studied. Due to the inhibitors present in the hydrolysate, several dilution levels and detoxification treatments, including overliming, activated charcoal adsorption, and resin adsorption, were evaluated for their effectiveness in relieving the inhibition on fermentation. Detoxification using resin and evaporation was found to be the most effective method in reducing the toxicity of WPH. ABE production in batch fermentation by Clostridium beijerinckii increased 68%, from 6.73 g/L in the non-treated and non-diluted WPH to 11.35 g/L in the resin treated WPH. With gasmore » stripping for in situ product removal, ABE production from WPH increased to 17.73 g/L, demonstrating that gas stripping was effective in alleviating butanol toxicity by selectively separating butanol from the fermentation broth, which greatly improved solvents production and sugar conversion in the fermentation. (C) 2013 Elsevier Ltd. All rights reserved.« less

Butanol production from wood pulping hydrolysate in an integrated fermentation-gas stripping process.

PubMed

Lu, Congcong; Dong, Jie; Yang, Shang-Tian

2013-09-01

Wood pulping hydrolysate (WPH) containing mainly xylose and glucose as a potential substrate for acetone-butanol-ethanol (ABE) fermentation was studied. Due to the inhibitors present in the hydrolysate, several dilution levels and detoxification treatments, including overliming, activated charcoal adsorption, and resin adsorption, were evaluated for their effectiveness in relieving the inhibition on fermentation. Detoxification using resin and evaporation was found to be the most effective method in reducing the toxicity of WPH. ABE production in batch fermentation by Clostridium beijerinckii increased 68%, from 6.73 g/L in the non-treated and non-diluted WPH to 11.35 g/L in the resin treated WPH. With gas stripping for in situ product removal, ABE production from WPH increased to 17.73 g/L, demonstrating that gas stripping was effective in alleviating butanol toxicity by selectively separating butanol from the fermentation broth, which greatly improved solvents production and sugar conversion in the fermentation. Copyright © 2013 Elsevier Ltd. All rights reserved.

Operant psychology goes to the fair: Marian and Keller Breland in the popular press, 1947-1966

PubMed Central

Bailey, Robert E.; Gillaspy, J. Arthur

2005-01-01

Marian and Keller Breland pioneered the application of operant psychology to commercial animal training during the 1940s and 1950s. The Brelands' story is relatively unknown in the history of behavior analysis. Using information from the Breland-Bailey papers, this paper describes the development and activities of Animal Behavior Enterprises (ABE), the Brelands' animal training business. We also review popular press coverage of the Brelands between 1947 and 1966 to investigate the level of public exposure to ABE-trained animals and to the principles and methods of operant psychology. An examination of 308 popular print articles featuring the Brelands indicates that there was public exposure of behavior analysis through the popular press coverage of ABE-trained animals. Furthermore, the expansion of operant methods to the marine mammal and bird training industries can be linked to the Brelands' mass media exposure. ImagesFigure 1Figure 2Figure 3Figure 4Figure 5 PMID:22478446

Butanol production from lignocellulose by simultaneous fermentation, saccharification, and pervaporation or vacuum evaporation.

PubMed

Díaz, Víctor Hugo Grisales; Tost, Gerard Olivar

2016-10-01

Techno-economic study of acetone, butanol and ethanol (ABE) fermentation from lignocellulose was performed. Simultaneous saccharification, fermentation and vacuum evaporation (SFS-V) or pervaporation (SFS-P) were proposed. A kinetic model of metabolic pathways for ABE fermentation with the effect of phenolics and furans in the growth was proposed based on published laboratory results. The processes were optimized in Matlab®. The end ABE purification was carried out by heat-integrated distillation. The objective function of the minimization was the total annualized cost (TAC). Fuel consumption of SFS-P using poly[1-(trimethylsilyl)-1-propyne] membrane was between 13.8 and 19.6% lower than SFS-V. Recovery of furans and phenolics for the hybrid reactors was difficult for its high boiling point. TAC of SFS-P was increased 1.9 times with supplementation of phenolics and furans to 3g/l each one for its high toxicity. Therefore, an additional detoxification method or an efficient pretreatment process will be necessary. Copyright © 2016 Elsevier Ltd. All rights reserved.

Mechanistic simulation of batch acetone-butanol-ethanol (ABE) fermentation with in situ gas stripping using Aspen Plus™.

PubMed

Darkwah, Kwabena; Nokes, Sue E; Seay, Jeffrey R; Knutson, Barbara L

2018-05-22

Process simulations of batch fermentations with in situ product separation traditionally decouple these interdependent steps by simulating a separate "steady state" continuous fermentation and separation units. In this study, an integrated batch fermentation and separation process was simulated for a model system of acetone-butanol-ethanol (ABE) fermentation with in situ gas stripping, such that the fermentation kinetics are linked in real-time to the gas stripping process. A time-dependent cell growth, substrate utilization, and product production is translated to an Aspen Plus batch reactor. This approach capitalizes on the phase equilibria calculations of Aspen Plus to predict the effect of stripping on the ABE fermentation kinetics. The product profiles of the integrated fermentation and separation are shown to be sensitive to gas flow rate, unlike separate steady state fermentation and separation simulations. This study demonstrates the importance of coupled fermentation and separation simulation approaches for the systematic analyses of unsteady state processes.

A comparison of the energy use of in situ product recovery techniques for the Acetone Butanol Ethanol fermentation.

PubMed

Outram, Victoria; Lalander, Carl-Axel; Lee, Jonathan G M; Davis, E Timothy; Harvey, Adam P

2016-11-01

The productivity of the Acetone Butanol Ethanol (ABE) fermentation can be significantly increased by application of various in situ product recovery (ISPR) techniques. There are numerous technically viable processes, but it is not clear which is the most economically viable in practice. There is little available information about the energy requirements and economics of ISPR for the ABE fermentation. This work compares various ISPR techniques based on UniSim process simulations of the ABE fermentation. The simulations provide information on the process energy and separation efficiency, which is fed into an economic assessment. Perstraction was the only technique to reduce the energy demand below that of a batch process, by approximately 5%. Perstraction also had the highest profit increase over a batch process, by 175%. However, perstraction is an immature technology, so would need significant development before being integrated to an industrial process. Copyright © 2016 Elsevier Ltd. All rights reserved.

An Expressive, Lightweight and Secure Construction of Key Policy Attribute-Based Cloud Data Sharing Access Control

NASA Astrophysics Data System (ADS)

Lin, Guofen; Hong, Hanshu; Xia, Yunhao; Sun, Zhixin

2017-10-01

Attribute-based encryption (ABE) is an interesting cryptographic technique for flexible cloud data sharing access control. However, some open challenges hinder its practical application. In previous schemes, all attributes are considered as in the same status while they are not in most of practical scenarios. Meanwhile, the size of access policy increases dramatically with the raise of its expressiveness complexity. In addition, current research hardly notices that mobile front-end devices, such as smartphones, are poor in computational performance while too much bilinear pairing computation is needed for ABE. In this paper, we propose a key-policy weighted attribute-based encryption without bilinear pairing computation (KP-WABE-WB) for secure cloud data sharing access control. A simple weighted mechanism is presented to describe different importance of each attribute. We introduce a novel construction of ABE without executing any bilinear pairing computation. Compared to previous schemes, our scheme has a better performance in expressiveness of access policy and computational efficiency.

Hydrophilic interaction liquid chromatography of anthranilic acid-labelled oligosaccharides with a 4-aminobenzoic acid ethyl ester-labelled dextran hydrolysate internal standard.

PubMed

Neville, David C A; Alonzi, Dominic S; Butters, Terry D

2012-04-13

Hydrophilic interaction liquid chromatography (HILIC) of fluorescently labelled oligosaccharides is used in many laboratories to analyse complex oligosaccharide mixtures. Separations are routinely performed using a TSK gel-Amide 80 HPLC column, and retention times of different oligosaccharide species are converted to glucose unit (GU) values that are determined with reference to an external standard. However, if retention times were to be compared with an internal standard, consistent and more accurate GU values would be obtained. We present a method to perform internal standard-calibrated HILIC of fluorescently labelled oligosaccharides. The method relies on co-injection of 4-aminobenzoic acid ethyl ester (4-ABEE)-labelled internal standard and detection by UV absorption, with 2-AA (2-aminobenzoic acid)-labelled oligosaccharides. 4-ABEE is a UV chromophore and a fluorophore, but there is no overlap of the fluorescent spectrum of 4-ABEE with the commonly used fluorescent reagents. The dual nature of 4-ABEE allows for accurate calculation of the delay between UV and fluorescent signals when determining the GU values of individual oligosaccharides. The GU values obtained are inherently more accurate as slight differences in gradients that can influence retention are negated by use of an internal standard. Therefore, this paper provides the first method for determination of HPLC-derived GU values of fluorescently labelled oligosaccharides using an internal calibrant. Copyright © 2012 Elsevier B.V. All rights reserved.

Global earthquake catalogs and long-range correlation of seismic activity (Invited)

NASA Astrophysics Data System (ADS)

Ogata, Y.

2009-12-01

In view of the long-term seismic activity in the world, homogeneity of a global catalog is indispensable. Lately, Engdahl and Villaseñor (2002) compiled a global earthquake catalog of magnitude (M)7.0 or larger during the last century (1900-1999). This catalog is based on the various existing catalogs such as Abe catalog (Abe, 1981, 1984; Abe and Noguchi, 1983a, b) for the world seismicity (1894-1980), its modified catalogs by Perez and Scholz (1984) and by Pacheco and Sykes (1992), and also the Harvard University catalog since 1975. However, the original surface wave magnitudes of Abe catalog were systematically changed by Perez and Scholz (1984) and Pacheco and Sykes (1992). They suspected inhomogeneity of the Abe catalog and claimed that the two seeming changes in the occurrence rate around 1922 and 1948 resulted from magnitude shifts for some instrumental-related reasons. They used a statistical test assuming that such a series of large earthquakes in the world should behave as the stationary Poisson process (uniform occurrences). It is obvious that their claim strongly depends on their a priori assumption of an independent or short-range dependence of earthquake occurrence. We question this assumption from the viewpoint of long-range dependence of seismicity. We make some statistical analyses of the spectrum, dispersion-time diagrams and R/S for estimating and testing of the long-range correlations. We also attempt to show the possibility that the apparent rate change in the global seismicity can be simulated by a certain long-range correlated process. Further, if we divide the globe into the two regions of high and low latitudes, for example, we have different shapes of the cumulative curves to each other, and the above mentioned apparent change-points disappear from the both regions. This suggests that the Abe catalog shows the genuine seismic activity rather than the artifact of the suspected magnitude shifts that should appear in any wide enough regions. We also use a local catalog for the wide regional area around Japan (Utsu, 1982a, b; Japan Meteorological Agency) which covers the period of 1885-1999, complete with M>=6.0 and occupies about 10% of the world seismicity. The synchronous variation of seismic frequency in the high latitude area of the world and in the regional area around Japan obtained from the independent catalogs is suggestive of an external effect such as a large-scale motion of the earth rather than the presupposed inhomogeneity of the catalogs.

Control of optical systems

NASA Technical Reports Server (NTRS)

Founds, D.

1988-01-01

Some of the current and planned activities at the Air Force Systems Command in structures and controls for optical-type systems are summarized. Many of the activities are contracted to industry; one task is an in-house program which includes a hardware test program. The objective of the in-house program, referred to as the Aluminum Beam Expander Structure (ABES), is to address issues involved in on-orbit system identification. The structure, which appears similar to the LDR backup structure, is about 35 feet tall. The activity to date has been limited to acquisition of about 250 hours of test data. About 30 hours of data per excitation force is gathered in order to obtain sufficient data for a good statistical estimate of the structural parameters. The development of an Integrated Structural Modeling (ISM) computer program is being done by Boeing Aerospace Company. The objective of the contracted effort is to develop a combined optics, structures, thermal, controls, and multibody dynamics simulation code.

Rare earth and other elements in components of the Abee enstatite chondrite

NASA Technical Reports Server (NTRS)

Frazier, R. M.; Boynton, W. V.

1985-01-01

Radiochemical and instrumental neutron activation analyses of REEs and other elements have been conducted for Abee clast samples, a matrix sample, a dark inclusion, magnetic and nonmagnetic samples, and bulk samples. Correlations of the REEs and oldhamite abundance for both the clasts and dark inclusions indicate that the REEs chiefly occur in oldhamite. The similar REE patterns for clasts and dark inclusions, and the similar mineral composition of oldhamite in clast and dark inclusions, suggest that the oldhamite in both the clasts and dark inclusions is of a common origin.

Particle Simulation of Coulomb Collisions: Comparing the Methods of Takizuka & Abe and Nanbu

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, C; Lin, T; Caflisch, R

2007-05-22

The interactions of charged particles in a plasma are in a plasma is governed by the long-range Coulomb collision. We compare two widely used Monte Carlo models for Coulomb collisions. One was developed by Takizuka and Abe in 1977, the other was developed by Nanbu in 1997. We perform deterministic and stochastic error analysis with respect to particle number and time step. The two models produce similar stochastic errors, but Nanbu's model gives smaller time step errors. Error comparisons between these two methods are presented.

Improving the management of people with a family history of breast cancer in primary care: before and after study of audit-based education

PubMed Central

2013-01-01

Background In England, guidance from National Institute for Clinical Excellence (NICE) states women with a family history of breast cancer presenting to primary care should be reassured or referred. We reviewed the evidence for interventions that might be applied in primary care and conducted an audit of whether low risk women are correctly advised and flagged. Methods We conducted a literature review to identify modifiable risk factors. We extracted routinely collected data from the computerised medical record systems of 6 general practices (population approximately 30,000); of the variables identified in the guidance. We implemented a quality improvement (QI) intervention called audit-based education (ABE) comparing participant practices with guidelines and each other before and after; we report odds ratios (OR) of any change in data recording. Results The review revealed evidence for advising on: diet, weight control, physical exercise, and alcohol. The proportion of patients with recordings of family history of: disease, neoplasms, and breast cancer were: 39.3%, 5.1% and 1.3% respectively. There was no significant change in the recording of family history of disease or cancer; OR 1.02 (95% CI 0.98-1.06); and 1.08 (95% CI 0.99-1.17) respectively. Recording of alcohol consumption and smoking both increased significantly; OR 1.36 (95% CI 1.30-1.43); and 1.42 (95% CI 1.27-1.60) respectively. Recording lifestyle advice fell; OR 0.84 (95% CI 0.81-0.88). Conclusions The study informs about current data recording and willingness to engage in ABE. Recording of risk factors improved after the intervention. Further QI is needed to achieve adherence to current guidance. PMID:23879178

Assessment of preventive measures for accidental blood exposure in operating theaters: a survey of 20 hospitals in Northern France.

PubMed

Tarantola, Arnaud; Golliot, Franck; L'Heriteau, François; Lebascle, Karin; Ha, Catherine; Farret, Danièle; Bignon, Sylvie; Smaïl, Amar; Doutrellot-Philippon, Catherine; Astagneau, Pascal; Bouvet, Elisabeth

2006-08-01

Accidental exposures to blood of body fluids (ABE) expose health care workers (HCW) to the risk of occupational infection. Our aim was to assess the prevention equipment available in the operating theater (OT) with reference to guidelines or recommendations and its use by the staff in that OT on that day and past history of ABE. Correspondents of the Centre de Coordination de la Lutte contre les Infections Nosocomiales (CCLIN) Paris-Nord ABE Surveillance Taskforce carried out an observational multicenter survey in 20 volunteer French hospitals. In total, 260 operating staff (including 151 surgeons) were investigated. Forty-nine of the 260 (18.8%) staff said they double-gloved for all patients and procedures, changing gloves hourly. Blunt-tipped suture needles were available in 49.1% of OT; 42 of 76 (55.3%) of the surgeons in these OT said they never used them. Overall, 60% and 64% of surgeons had never self-tested for HIV and hepatitis C virus (HCV), respectively. Fifty-five surgeons said they had sustained a total of 96 needlestick injuries during the month preceding the survey. Ten of these surgeons had notified of 1 needlestick injury each to the occupational health department of their hospital (notification rate, 10.4%). The occurrence of needlestick injury remained high in operating personnel in France in 2000. Although hospitals may improve access to protective devices, operating staff mindful of safety in the OT should increase their use of available devices, their knowledge of their own serostatus, and their ABE notification rate to guide well-targeted prevention efforts.

Japan's 2014 General Election: Political Bots, Right-Wing Internet Activism, and Prime Minister Shinzō Abe's Hidden Nationalist Agenda.

PubMed

Schäfer, Fabian; Evert, Stefan; Heinrich, Philipp

2017-12-01

In this article, we present results on the identification and behavioral analysis of social bots in a sample of 542,584 Tweets, collected before and after Japan's 2014 general election. Typical forms of bot activity include massive Retweeting and repeated posting of (nearly) the same message, sometimes used in combination. We focus on the second method and present (1) a case study on several patterns of bot activity, (2) methodological considerations on the automatic identification of such patterns and the prerequisite near-duplicate detection, and (3) we give qualitative insights into the purposes behind the usage of social/political bots. We argue that it was in the latency of the semi-public sphere of social media-and not in the visible or manifest public sphere (official campaign platform, mass media)-where Shinzō Abe's hidden nationalist agenda interlocked and overlapped with the one propagated by organizations such as Nippon Kaigi and Internet right-wingers (netto uyo) during the election campaign, the latter potentially forming an enormous online support army of Abe's agenda.

Japan's 2014 General Election: Political Bots, Right-Wing Internet Activism, and Prime Minister Shinzō Abe's Hidden Nationalist Agenda

PubMed Central

Schäfer, Fabian; Evert, Stefan; Heinrich, Philipp

2017-01-01

Abstract In this article, we present results on the identification and behavioral analysis of social bots in a sample of 542,584 Tweets, collected before and after Japan's 2014 general election. Typical forms of bot activity include massive Retweeting and repeated posting of (nearly) the same message, sometimes used in combination. We focus on the second method and present (1) a case study on several patterns of bot activity, (2) methodological considerations on the automatic identification of such patterns and the prerequisite near-duplicate detection, and (3) we give qualitative insights into the purposes behind the usage of social/political bots. We argue that it was in the latency of the semi-public sphere of social media—and not in the visible or manifest public sphere (official campaign platform, mass media)—where Shinzō Abe's hidden nationalist agenda interlocked and overlapped with the one propagated by organizations such as Nippon Kaigi and Internet right-wingers (netto uyo) during the election campaign, the latter potentially forming an enormous online support army of Abe's agenda. PMID:29182493

Enhancing acetone biosynthesis and acetone-butanol-ethanol fermentation performance by co-culturing Clostridium acetobutylicum/Saccharomyces cerevisiae integrated with exogenous acetate addition.

PubMed

Luo, Hongzhen; Ge, Laibing; Zhang, Jingshu; Ding, Jian; Chen, Rui; Shi, Zhongping

2016-01-01

Acetone is the major by-product in ABE fermentations, most researches focused on increasing butanol/acetone ratio by decreasing acetone biosynthesis. However, economics of ABE fermentation industry strongly relies on evaluating acetone as a valuable platform chemical. Therefore, a novel ABE fermentation strategy focusing on bio-acetone production by co-culturing Clostridium acetobutylicum/Saccharomyces cerevisiae with exogenous acetate addition was proposed. Experimental and theoretical analysis revealed the strategy could, enhance C. acetobutylicum survival oriented amino acids assimilation in the cells; control NADH regeneration rate at moderately lower level to enhance acetone synthesis but without sacrificing butanol production; enhance the utilization ability of C. acetobutylicum on glucose and direct most of extra consumed glucose into acetone/butanol synthesis routes. By implementing the strategy using synthetic or acetate fermentative supernatant, acetone concentrations increased to 8.27-8.55g/L from 5.86g/L of the control, while butanol concentrations also elevated to the higher levels of 13.91-14.23g/L from 11.63g/L simultaneously. Copyright © 2015 Elsevier Ltd. All rights reserved.

Enhanced enzymatic hydrolysis and acetone-butanol-ethanol fermentation of sugarcane bagasse by combined diluted acid with oxidate ammonolysis pretreatment.

PubMed

Li, Hailong; Xiong, Lian; Chen, Xuefang; Wang, Can; Qi, Gaoxiang; Huang, Chao; Luo, Mutan; Chen, Xinde

2017-03-01

This study aims to propose a biorefinery pretreatment technology for the bioconversion of sugarcane bagasse (SB) into biofuels and N-fertilizers. Performance of diluted acid (DA), aqueous ammonia (AA), oxidate ammonolysis (OA) and the combined DA with AA or OA were compared in SB pretreatment by enzymatic hydrolysis, structural characterization and acetone-butanol-ethanol (ABE) fermentation. Results indicated that DA-OA pretreatment improves the digestibility of SB by sufficiently hydrolyzing hemicellulose into fermentable monosaccharides and oxidating lignin into soluble N-fertilizer with high nitrogen content (11.25%) and low C/N ratio (3.39). The enzymatic hydrolysates from DA-OA pretreated SB mainly composed of glucose was more suitable for the production of ABE solvents than the enzymatic hydrolysates from OA pretreated SB containing high ratio of xylose. The fermentation of enzymatic hydrolysates from DA-OA pretreated SB produced 12.12g/L ABE in 120h. These results suggested that SB could be utilized efficient, economic, and environmental by DA-OA pretreatment. Copyright © 2017 Elsevier Ltd. All rights reserved.

Optimization of wastewater microalgae saccharification using dilute acid hydrolysis for acetone, butanol, and ethanol fermentation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Castro, Yessica; Ellis, Joshua T.; Miller, Charles D.

2015-02-01

Exploring and developing sustainable and efficient technologies for biofuel production are crucial for averting global consequences associated with fuel shortages and climate change. Optimization of sugar liberation from wastewater algae through acid hydrolysis was determined for subsequent fermentation to acetone, butanol, and ethanol (ABE) by Clostridium saccharoperbutylacetonicum N1-4. Acid concentration, retention time, and temperature were evaluated to determine optimal hydrolysis conditions by assessing the sugar and ABE yield as well as the associated costs. Sulfuric acid concentrations ranging from 0-1.5 M, retention times of 40-120 min, and temperatures from 23°C- 90°C were combined to form a full factorial experiment. Acidmore » hydrolysis pretreatment of 10% dried wastewater microalgae using 1.0 M sulfuric acid for 120 min at 80-90°C was found to be the optimal parameters, with a sugar yield of 166.1 g for kg of dry algae, concentrations of 5.23 g/L of total ABE, and 3.74 g/L of butanol at a rate of USD $12.83 per kg of butanol.« less

Aqueous biphasic extraction of uranium and thorium from contaminated soils. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chaiko, D.J.; Gartelmann, J.; Henriksen, J.L.

1995-07-01

The aqueous biphasic extraction (ABE) process for soil decontamination involves the selective partitioning of solutes and fine particulates between two immiscible aqueous phases. The biphase system is generated by the appropriate combination of a water-soluble polymer (e.g., polyethlene glycol) with an inorganic salt (e.g., sodium carbonate). Selective partitioning results in 99 to 99.5% of the soil being recovered in the cleaned-soil fraction, while only 0.5 to 1% is recovered in the contaminant concentrate. The ABE process is best suited to the recovery of ultrafine, refractory material from the silt and clay fractions of soils. During continuous countercurrent extraction tests withmore » soil samples from the Fernald Environmental Management Project site (Fernald, OH), particulate thorium was extracted and concentrated between 6- and 16-fold, while the uranium concentration was reduced from about 500 mg/kg to about 77 mg/kg. Carbonate leaching alone was able to reduce the uranium concentration only to 146 mg/kg. Preliminary estimates for treatment costs are approximately $160 per ton of dry soil. A detailed flowsheet of the ABE process is provided.« less

Acetone-butanol-ethanol (ABE) fermentation using Clostridium acetobutylicum XY16 and in situ recovery by PDMS/ceramic composite membrane.

PubMed

Wu, Hao; Chen, Xiao-Peng; Liu, Gong-Ping; Jiang, Min; Guo, Ting; Jin, Wan-Qin; Wei, Ping; Zhu, Da-Wei

2012-09-01

PDMS/ceramic composite membrane was directly integrated with acetone-butanol-ethanol (ABE) fermentation using Clostridium acetobutylicum XY16 at 37 °C and in situ removing ABE from fermentation broth. The membrane was integrated with batch fermentation, and approximately 46 % solvent was extracted. The solvent in permeates was 118 g/L, and solvent productivity was 0.303 g/(L/h), which was approximately 33 % higher compared with the batch fermentation without in situ recovery. The fed-batch fermentation with in situ recovery by pervaporation continued for more than 200 h, 61 % solvent was extracted, and the solvent in penetration was 96.2 g/L. The total flux ranged from 0.338 to 0.847 kg/(m(2)/h) and the separation factor of butanol ranged from 5.1 to 27.1 in this process. The membrane was fouled by the active fermentation broth, nevertheless the separation performances were partially recovered by offline membrane cleaning, and the solvent productivity was increased to 0.252 g/(L/h), which was 19 % higher compared with that in situ recovery process without membrane cleaning.

Efficient acetone-butanol-ethanol production by Clostridium beijerinckii from sugar beet pulp.

PubMed

Bellido, Carolina; Infante, Celia; Coca, Mónica; González-Benito, Gerardo; Lucas, Susana; García-Cubero, María Teresa

2015-08-01

Sugar beet pulp (SBP) has been investigated as a promising feedstock for ABE fermentation by Clostridium beijerinckii. Although lignin content in SBP is low, a pretreatment is needed to enhance enzymatic hydrolysis and fermentation yields. Autohydrolysis at pH 4 has been selected as the best pretreatment for SBP in terms of sugars release and acetone and butanol production. The best overall sugars release yields from raw SBP ranged from 66.2% to 70.6% for this pretreatment. The highest ABE yield achieved was 0.4g/g (5.1g/L of acetone and 6.6g/L butanol) and 143.2g ABE/kg SBP (62.3g acetone and 80.9g butanol) were obtained when pretreated SBP was enzymatically hydrolyzed at 7.5% (w/w) solid loading. Higher solid loadings (10%) offered higher acetone and butanol titers (5.8g/L of acetone and 7.8g/L butanol). All the experiments were carried out under not-controlling pH conditions reaching about 5.3 in the final samples. Copyright © 2015 Elsevier Ltd. All rights reserved.

What drives quality improvement in chronic kidney disease (CKD) in primary care: process evaluation of the Quality Improvement in Chronic Kidney Disease (QICKD) trial.

PubMed

Nihat, Akin; de Lusignan, Simon; Thomas, Nicola; Tahir, Mohammad Aumran; Gallagher, Hugh

2016-04-06

This study is a process evaluation of the Quality Improvement in Chronic Kidney Disease (QICKD) study, comparing audit-based education (ABE) and sending clinical guidelines and prompts (G&P) with usual practice, in improving systolic blood pressure control in primary care. This evaluation aimed to explore how far clinical staff in participating practices were aware of the intervention, and why change in practice might have taken place. 4 primary care practices in England: 2 received ABE, and 2 G&P. We purposively selected 1 northern/southern/city and rural practice from each study arm (from a larger pool of 132 practices as part of the QICKD trial). The 4 study practices were purposively sampled, and focus groups conducted with staff from each. All staff members were invited to attend. Focus groups in each of 4 practices, at the mid-study point and at the end. 4 additional trial practices not originally selected for in-depth process evaluation took part in end of trial focus groups, to a total of 12 focus groups. These were recorded, transcribed and analysed using the framework approach. 5 themes emerged: (1) involvement in the study made participants more positive about the CKD register; (2) clinicians did not always explain to patients that they had CKD; (3) while practitioners improved their monitoring of CKD, many were sceptical that it improved care and were more motivated by pay-for-performance measures; (4) the impact of study interventions on practice was generally positive, particularly the interaction with specialists, included in ABE; (5) the study stimulated ideas for future clinical practice. Improving quality in CKD is complex. Lack of awareness of clinical guidelines and scepticism about their validity are barriers to change. While pay-for-performance incentives are the main driver for change, quality improvement interventions can have a complementary influence. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

Pervaporation of model acetone-butanol-ethanol fermentation product solutions using polytetrafluoroethylene membranes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vrana, D.L.; Meagher, M.M.; Hutkins, R.W.

1993-10-01

A pervaporation apparatus was designed and tested in an effort to develop an integrated fermentation and product recovery process for acetone-butanol-ethanol(ABE) fermentation. A crossflow membrane module able to accommodate flat sheet hydrophobic membranes was used for the experiments. Permeate vapors were collected under vacuum and condensed in a dry ice/ethanol cold trap. The apparatus containing polytetrafluoroethylene membranes was tested using butanol-water and model solutions of ABE products. Parameters such as product concentration, component effect, temperature, and permeate side pressure were examined. 25 refs., 3 figs., 5 tabs.

An Explorer-Class Astrobiology Mission

NASA Technical Reports Server (NTRS)

Sandford, Scott; Greene, Thomas; Allamandola, Louis; Arno, Roger; Bregman, Jesse; Cox, Sylvia; Davis, Paul K.; Gonzales, Andrew; Haas, Michael; Hanel, Robert;

Applications of AFM for atomic manipulation and spectroscopy

NASA Astrophysics Data System (ADS)

Custance, Oscar

2009-03-01

Since the first demonstration of atom-by-atom assembly [1], atomic manipulation with scanning tunneling microscopy has yielded stunning realizations in nanoscience. A new exciting panorama has been recently opened with the possibility of manipulating atoms at surfaces using atomic force microscopy (AFM) [2-5]. In this talk, we will present two different approaches that enable patterning structures at semiconductor surfaces by manipulating individual atoms with AFM and at room temperature [2, 3]. We will discuss the physics behind each protocol through the analysis of the measured forces associated with these manipulations [3-5]. Another challenging issue in scanning probe microscopy is the ability to disclose the local chemical composition of a multi-element system at atomic level. Here, we will introduce a single-atom chemical identification method, which is based on detecting the forces between the outermost atom of the AFM tip and the atoms at a surface [6]. We demonstrate this identification procedure on a particularly challenging system, where any discrimination attempt based solely on topographic measurements would be impossible to achieve. [4pt] References: [0pt] [1] D. M. Eigler and E. K. Schweizer, Nature 344, 524 (1990); [0pt] [2] Y. Sugimoto, M. Abe, S. Hirayama, N. Oyabu, O. Custance and S. Morita, Nature Materials 4, 156 (2005); [0pt] [3] Y. Sugimoto, P. Pou, O. Custance, P. Jelinek, M. Abe, R. Perez and S. Morita, Science 322, 413 (2008); [0pt] [4] Y. Sugimoto, P. Jelinek, P. Pou, M. Abe, S. Morita, R. Perez and O. Custance, Phys. Rev. Lett. 98, 106104 (2007); [0pt] [5] M. Ternes, C. P. Lutz, C. F. Hirjibehedin, F. J. Giessibl and A. J. Heinrich, Science 319, 1066 (2008); [0pt] [6] Y. Sugimoto, P. Pou, M. Abe, P. Jelinek, R. Perez, S. Morita, and O. Custance, Nature 446, 64 (2007)

Assessment of potential life-cycle energy and greenhouse gas emission effects from using corn-based butanol as a transportation fuel.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu, M.; Wang, M.; Liu, J.

2008-01-01

Since advances in the ABE (acetone-butanol-ethanol) fermentation process in recent years have led to significant increases in its productivity and yields, the production of butanol and its use in motor vehicles have become an option worth evaluating. This study estimates the potential life-cycle energy and emission effects associated with using bio-butanol as a transportation fuel. It employs a well-to-wheels (WTW) analysis tool: the Greenhouse Gases, Regulated Emissions, and Energy Use in Transportation (GREET) model. The estimates of life-cycle energy use and greenhouse gas (GHG) emissions are based on an Aspen Plus(reg. sign) simulation for a corn-to-butanol production process, which describesmore » grain processing, fermentation, and product separation. Bio-butanol-related WTW activities include corn farming, corn transportation, butanol production, butanol transportation, and vehicle operation. In this study, we also analyzed the bio-acetone that is coproduced with bio-butanol as an alternative to petroleum-based acetone. We then compared the results for bio-butanol with those of conventional gasoline. Our study shows that driving vehicles fueled with corn-based butanol produced by the current ABE fermentation process could result in substantial fossil energy savings (39%-56%) and avoid large percentage of the GHG emission burden, yielding a 32%-48% reduction relative to using conventional gasoline. On energy basis, a bushel of corn produces less liquid fuel from the ABE process than that from the corn ethanol dry mill process. The coproduction of a significant portion of acetone from the current ABE fermentation presents a challenge. A market analysis of acetone, as well as research and development on robust alternative technologies and processes that minimize acetone while increase the butanol yield, should be conducted.« less

Allopurinol-mediated lignocellulose-derived microbial inhibitor tolerance by Clostridium beijerinckii during acetone-butanol-ethanol (ABE) fermentation.

PubMed

Ujor, Victor; Agu, Chidozie Victor; Gopalan, Venkat; Ezeji, Thaddeus Chukwuemeka

2015-04-01

In addition to glucans, xylans, and arabinans, lignocellulosic biomass hydrolysates contain significant levels of nonsugar components that are toxic to the microbes that are typically used to convert biomass to biofuels and chemicals. To enhance the tolerance of acetone-butanol-ethanol (ABE)-generating Clostridium beijerinckii NCIMB 8052 to these lignocellulose-derived microbial inhibitory compounds (LDMICs; e.g., furfural), we have been examining different metabolic perturbation strategies to increase the cellular reductant pools and thereby facilitate detoxification of LDMICs. As part of these efforts, we evaluated the effect of allopurinol, an inhibitor of NAD(P)H-generating xanthine dehydrogenase (XDH), on C. beijerinckii grown in furfural-supplemented medium and found that it unexpectedly increased the rate of detoxification of furfural by 1.4-fold and promoted growth, butanol, and ABE production by 1.2-, 2.5-, and 2-fold, respectively. Since NAD(P)H/NAD(P)(+) levels in C. beijerinckii were largely unchanged upon allopurinol treatment, we postulated and validated a possible basis in DNA repair to account for the solventogenic gains with allopurinol. Following the observation that supplementation of allopurinol in the C. beijerinckii growth media mitigates the toxic effects of nalidixic acid, a DNA-damaging antibiotic, we found that allopurinol elicited 2.4- and 6.7-fold increase in the messenger RNA (mRNA) levels of xanthine and hypoxanthine phosphoribosyltransferases, key purine-salvage enzymes. Consistent with this finding, addition of inosine (a precursor of hypoxanthine) and xanthine led to 1.4- and 1.7-fold increase in butanol production in furfural-challenged cultures of C. beijerinckii. Taken together, our results provide a purine salvage-based rationale for the unanticipated effect of allopurinol in improving furfural tolerance of the ABE-fermenting C. beijerinckii.

Assessment of potential life-cycle energy and greenhouse gas emission effects from using corn-based butanol as a transportation fuel.

PubMed

Wu, May; Wang, Michael; Liu, Jiahong; Huo, Hong

2008-01-01

Since advances in the ABE (acetone-butanol-ethanol) fermentation process in recent years have led to significant increases in its productivity and yields, the production of butanol and its use in motor vehicles have become an option worth evaluating. This study estimates the potential life-cycle energy and emission effects associated with using bio-butanol as a transportation fuel. It employs a well-to-wheels (WTW) analysis tool: the Greenhouse Gases, Regulated Emissions, and Energy Use in Transportation (GREET) model. The estimates of life-cycle energy use and greenhouse gas (GHG) emissions are based on an Aspen Plus(R) simulation for a corn-to-butanol production process, which describes grain processing, fermentation, and product separation. Bio-butanol-related WTW activities include corn farming, corn transportation, butanol production, butanol transportation, and vehicle operation. In this study, we also analyzed the bio-acetone that is coproduced with bio-butanol as an alternative to petroleum-based acetone. We then compared the results for bio-butanol with those of conventional gasoline. Our study shows that driving vehicles fueled with corn-based butanol produced by the current ABE fermentation process could result in substantial fossil energy savings (39%-56%) and avoid large percentage of the GHG emission burden, yielding a 32%-48% reduction relative to using conventional gasoline. On energy basis, a bushel of corn produces less liquid fuel from the ABE process than that from the corn ethanol dry mill process. The coproduction of a significant portion of acetone from the current ABE fermentation presents a challenge. A market analysis of acetone, as well as research and development on robust alternative technologies and processes that minimize acetone while increase the butanol yield, should be conducted.

Factor structure of the arthritis body experience scale (ABES) in a U.S. population of people with osteoarthritis (OA), rheumatoid arthritis (RA), fibromyalgia (FM) and other rheumatic conditions.

PubMed

Boyington, J E A; Devellis, R; Shreffler, J; Schoster, B; Callahan, L F

2008-01-01

To examine the psychometric properties of the Arthritis Body Experience Scale (ABES) in a US sample of people with osteoarthritis, rheumatoid arthritis, fibromyalgia and other rheumatic conditions. The ABES, with the scoring direction modified, was phone-administered to 937 individuals who self-identified as having one or more arthritis conditions based on a validated, US, national survey assessment tool. Descriptive statistics of demographic variables and factor analysis of scale items were conducted. Scale dimensionality was assessed using principal component analysis (PCA) with oblique rotation. Criteria for assessing factors were eigenvalues > 1, visual assessment of scree plot, and structure and pattern matrices. The predominantly female (74.2%) and Caucasian (79.9%) sample had a mean age of 61.0 ± 13.1 years, and a mean BMI of 30.2 ± 7.1. Major arthritis conditions reported were rheumatoid arthritis, osteoarthritis and fibromyalgia. A three-factor structure with cronbach alpha values of .84, .85 and .53 was elicited, and accounted for 72% of the variance. Compared to the two-factor structure evidenced by the original ABES scale in a sample of UK adults, the data from this sample evidenced a three-factor structure with higher variance. The third factor's cronbach alpha of .53 was low and could be improved by the addition of salient questions derived from further qualitative interviews with patients with arthritis and other rheumatic conditions and from current literature findings. The observed psychometrics indicate the scale usefully assesses body image in populations with arthritis and related conditions. However, further testing and refinement is needed to determine its utility in clinical and other settings.

Heterogeneous flow in multi-layer joint networks and its influence on incipient karst generation

NASA Astrophysics Data System (ADS)

Wang, X.; Jourde, H.

2017-12-01

Various dissolution types (e.g. pipe, stripe and sheet karstic features) have been observed in fractured layered limestones. Yet, due to a large range of structural and hydraulic parameters play a role in the karstification process, the dissolution mechanism, occurring either along fractures or bedding planes, is difficult to quantify. In this study, we use numerical models to investigate the influence of these parameters on the generation of different types of incipient karst. Specifically, we focus on two parameters: the fracture intensity contrast between adjacent layers and the aperture ratio between bedding planes and joints (abed/ajoint). The DFN models were generated using a pseudo-genetic code that considers the stress shadow zone. Flow simulations were performed using a combined finite-volume finite-element simulator under practical boundary conditions. The flow channeling within the fracture networks was characterized by applying a multi-fractal technique. The rock block equivalent permeability (keff) was also calculated to quantify the change in bulk hydraulic properties when changing the selected structural and hydraulic parameters. The flow simulation results show that the abed/ajoint ratio has a first-order control on the heterogeneous distribution of flow in the multi-layer system and on the magnitude of equivalent permeability. When abed/ajoint < 0.1, flow in the system is highly localized and controlled by joints, and the keff is low; while, when abed/ajoint > 0.1, the bedding plane has more control and flow becomes more pervasive and uniform, and the keff is accordingly high. A simple model, accounting for the calculation of the heterogeneous distributions of Damköhler number associated with different aperture ratios, is proposed to predict what type of incipient karst tends to develop under the studied flow conditions.

Bell-correlated activable bound entanglement in multiqubit systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bandyopadhyay, Somshubhro; Chattopadhyay, Indrani; Roychowdhury, Vwani

2005-06-15

We show that the Hilbert space of even number ({>=}4) of qubits can always be decomposed as a direct sum of four orthogonal subspaces such that the normalized projectors onto the subspaces are activable bound entangled (ABE) states. These states also show a surprising recursive relation in the sense that the states belonging to 2N+2 qubits are Bell correlated to the states of 2N qubits; hence, we refer to these states as Bell-correlated ABE (BCABE) states. We also study the properties of noisy BCABE states and show that they are very similar to that of two qubit Bell-diagonal states.

Effect of phosphoric acid pretreatment of corncobs on the fermentability of Clostridium beijerinckii TISTR 1461 for biobutanol production.

PubMed

Boonsombuti, Akarin; Luengnaruemitchai, Apanee; Wongkasemjit, Sujitra

2015-01-01

Corncobs pretreated with H2SO4, HNO3, and H3PO4 were compared to evaluate the fermentation ability of Clostridium beijerinckii TISTR 1461 to produce biobutanol via acetone-butanol-ethanol (ABE) fermentation. It was found that the hydrolysate from H3PO4 pretreatment could be used as a substrate without any inhibitor removal methods. However, in terms of sugar yield, it gave the lowest total sugars in both pretreatment and enzymatic hydrolysis. Response surface methodology was applied to optimize enzymatic hydrolysis of the pretreated corncobs. The optimized conditions reduced the consumption of enzymes and hydrolysis time to 7.68 FPU/g biomass and 63.88 hr, respectively, and yielded 51.82 g/L reducing sugars. The Celluclast 1.5 L and Novozyme 188 enzyme ratio were varied to maximize the hydrolyzed sugars. The ABE fermentation, using substrate from phosphoric acid pretreatment of corncobs, with 10 g/L glucose supplementation produced 11.64 g/L of total ABE, which was close to the control experiment using synthetic medium. This study showed that corncobs pretreated with phosphoric acid could potentially be used as a substrate without using a detoxification process.

Simultaneous fermentation and separation in an immobilized cell trickle bed reactor: Acetone-butanol-ethane (ABE) and ethanol fermentation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, C.H.

1989-01-01

A novel process employing immobilized cells and in-situ product removal was studied for acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum and ethanol fermentation by Saccharomyces cerevisiae. Experimental studies of ABE fermentation in a trickle bed reactor without product separation showed that solvent production could be improved by one order of magnitude compared to conventional batch fermentation. Control of effluent pH near 4.3 and feed glucose concentrations higher than 10 g/L were the necessary conditions for cell growth and solvent production. A mathematical model using an equilibrium staged model predicted efficient separation of butanol from the fermentation broth. Activity coefficients of multicomponentmore » system were estimated by Wilson's equation or the ASOG method. Inhibition by butanol and organic acids was incorporated into the kinetic expression. Experimental performance of simultaneous fermentation and separation in an immobilized cell trickle bed reactor showed that glucose conversion was improved as predicted by mathematical modeling and analysis. The effect of pH and temperature on ethanol fermentation by Saccharomyces cerevisiae was studied in free and immobilized cell reactors. Conditions for the highest glucose conversion, cell viability and least glycerol yield were determined.« less

Continuous production of butanol from starch-based packing peanuts.

PubMed

Ezeji, Thaddeus C; Groberg, Marisa; Qureshi, Nasib; Blaschek, Hans P

2003-01-01

Acetone, butanol, ethanol (ABE, or solvents) were produced from starch-based packing peanuts in batch and continuous reactors. In a batch reactor, 18.9 g/L of total ABE was produced from 80 g/L packing peanuts in 110 h of fermentation. The initial and final starch concentrations were 69.6 and 11.1 g/L, respectively. In this fermentation, ABE yield and productivity of 0.32 and 0.17 g/(L h) were obtained, respectively. Compared to the batch fermentation, continuous fermentation of 40 g/L of starchbased packing peanuts in P2 medium resulted in a maximum solvent production of 8.4 g/L at a dilution rate of 0.033 h-1. This resulted in a productivity of 0.27 g/(L h). However, the reactor was not stable and fermentation deteriorated with time. Continuous fermentation of 35 g/L of starch solution resulted in a similar performance. These studies were performed in a vertical column reactor using Clostridium beijerinckii BA101 and P2 medium. It is anticipated that prolonged exposure of culture to acrylamide, which is formed during boiling/autoclaving of starch, affects the fermentation negatively.

New Model Exhaust System Supports Testing in NASA Lewis' 10- by 10-Foot Supersonic Wind Tunnel

NASA Technical Reports Server (NTRS)

Roeder, James W., Jr.

1998-01-01

In early 1996, the ability to run NASA Lewis Research Center's Abe Silverstein 10- by 10- Foot Supersonic Wind Tunnel (10x10) at subsonic test section speeds was reestablished. Taking advantage of this new speed range, a subsonic research test program was scheduled for the 10x10 in the fall of 1996. However, many subsonic aircraft test models require an exhaust source to simulate main engine flow, engine bleed flows, and other phenomena. This was also true of the proposed test model, but at the time the 10x10 did not have a model exhaust capability. So, through an in-house effort over a period of only 5 months, a new model exhaust system was designed, installed, checked out, and made ready in time to support the scheduled test program.

Supervised Multi-Authority Scheme with Blind Signature for IoT with Attribute Based Encryption

NASA Astrophysics Data System (ADS)

Nissenbaum, O. V.; Ponomarov, K. Y.; Zaharov, A. A.

2018-04-01

This article proposes a three-side cryptographic scheme for verifying device attributes with a Supervisor and a Certification Authority (CA) for attribute-based encryption. Two options are suggested: using a message authentication code and using a digital signature. The first version is suitable for networks with one CA, and the second one for networks with several CAs, including dynamic systems. Also, the addition of this scheme with a blind signature is proposed to preserve the confidentiality of the device attributes from the CA. The introduction gives a definition and a brief historical overview of attribute-based encryption (ABE), addresses the use of ABE in the Internet of Things.

The investigation of the binding of 6-mercaptopurine to site I on human serum albumin.

PubMed

Sochacka, Jolanta; Baran, Wojciech

2012-12-01

6-Mercaptopurine (6-MP) is one of a large series of purine analogues which has been found active against human leukemias. The equilibrium dialysis, circular dichroism (CD) and molecular docking were employed to study the binding of 6-MP to human serum albumin (HSA). The binding of 6-MP to HSA in the equilibrium dialysis experiment was detected by measuring the displacement of 6-MP by specific markers for site I on HSA, warfarin (RWF), phenylbutazone (PhB) and n-butyl p-aminobenzoate (ABE). It was shown, according to CD data, that binding of 6-MP to HSA leads to alteration of HSA secondary structure. Based on the findings from displacement experiment and molecular docking simulation it was found that 6-MP was located within binding cavity of subdomain IIA and the space occupied by site markers overlapped with that of 6-MP. Displacement of 6-MP by the RWF or PhB was not up the level expected for a competitive mechanism, therefore displacement of 6-MP was rather by non-cooperative than that the direct competition. Instead, in case of the interaction between ABE and 6-MP, when the little enhancement of the binding of ABE by 6-MP was found, the interaction could be via a positively cooperative mechanism.

Evaluation of asymmetric polydimethylsiloxane-polyvinylidene fluoride composite membrane and incorporated with acetone-butanol-ethanol fermentation for butanol recovery.

PubMed

Xue, Chuang; Du, Guang-Qing; Chen, Li-Jie; Ren, Jian-Gang; Bai, Feng-Wu

2014-10-20

The polydimethylsiloxane-polyvinylidene fluoride (PDMS-PVDF) composite membrane was studied for its pervaporation performance to removal of butanol from butanol/ABE solution, fermentation broth as well as incorporated with acetone-butanol-ethanol (ABE) fermentation. The total flux and butanol titer in permeate through the PDMS-PVDF membrane were up to 769.6 g/m(2)h and 323.5 g/L at 80 °C, respectively. The butanol flux and total flux increased with increasing the feed temperature as well as the feed butanol titer. The butanol separation factor and butanol titer in permeate decreased slightly in the presence of acetone and ethanol in the feed due to their preferential dissolution and competitive permeation through the membrane. In fed-batch fermentation incorporated with pervaporation, butanol titer and flux in permeate maintained at a steady level with the range of 139.9-154.0 g/L and 13.3-16.3 g/m(2)h, respectively, which was attributed to the stable butanol titer in fermentation broth as well as the excellent hydrophobic nature of the PDMS-PVDF matrix. Therefore, the PDMS-PVDF composite membrane had a great potential in the in situ product recovery with ABE fermentation, enabling the economic production of biobutanol. Copyright © 2014 Elsevier B.V. All rights reserved.

Divided attention enhances the recognition of emotional stimuli: evidence from the attentional boost effect.

PubMed

Rossi-Arnaud, Clelia; Spataro, Pietro; Costanzi, Marco; Saraulli, Daniele; Cestari, Vincenzo

2018-01-01

The present study examined predictions of the early-phase-elevated-attention hypothesis of the attentional boost effect (ABE), which suggests that transient increases in attention at encoding, as instantiated in the ABE paradigm, should enhance the recognition of neutral and positive items (whose encoding is mostly based on controlled processes), while having small or null effects on the recognition of negative items (whose encoding is primarily based on automatic processes). Participants were presented a sequence of negative, neutral and positive stimuli (pictures in Experiment 1, words in Experiment 2) associated to target (red) squares, distractor (green) squares or no squares (baseline condition). They were told to attend to the pictures/words and simultaneously press the spacebar of the computer when a red square appeared. In a later recognition task, stimuli associated to target squares were recognised better than stimuli associated to distractor squares, replicating the standard ABE. More importantly, we also found that: (a) the memory enhancement following target detection occurred with all types of stimuli (neutral, negative and positive) and (b) the advantage of negative stimuli over neutral stimuli was intact in the DA condition. These findings suggest that the encoding of negative stimuli depends on both controlled (attention-dependent) and automatic (attention-independent) processes.

Examining the Relationships of Component Reading Skills to Reading Comprehension in Struggling Adult Readers: A Meta-Analysis

PubMed Central

Tighe, Elizabeth L.; Schatschneider, Christopher

2015-01-01

The current study employed a meta-analytic approach to investigate the relative importance of component reading skills to reading comprehension in struggling adult readers. A total of 10 component skills were consistently identified across 16 independent studies and 2,707 participants. Random effects models generated 76 predictor-reading comprehension effect sizes among the 10 constructs. The results indicated that six of the component skills exhibited strong relationships with reading comprehension (average rs ≥ .50): morphological awareness, language comprehension, fluency, oral vocabulary knowledge, real word decoding, and working memory. Three of the component skills yielded moderate relationships with reading comprehension (average rs ≥ .30 and < .50): pseudoword decoding, orthographic knowledge, and phonological awareness. Rapid automatized naming (RAN) was the only component skill that was weakly related to reading comprehension (r = .15). Morphological awareness was a significantly stronger correlate of reading comprehension than phonological awareness and RAN. This study provides the first attempt at a systematic synthesis of the recent research investigating the reading skills of adults with low literacy skills, a historically under-studied population. Directions for future research, the relation of our results to the children’s literature, and the implications for researchers and Adult Basic Education (ABE) programs are discussed. PMID:25350926

Hydrothermal crystal growth of ABe 2BO 3F 2 (A=K, Rb, Cs, Tl) NLO crystals

NASA Astrophysics Data System (ADS)

McMillen, Colin D.; Kolis, Joseph W.

2008-04-01

Crystals of a family of compounds, ABe 2BO 3F 2 (ABBF, A=K, Rb, Cs, Tl), have been grown hydrothermally. Each of these materials was studied using the powder SHG technique and exhibited promising NLO behavior. Seeded crystal growth was demonstrated and the growth conditions were optimized by modifying the temperature, thermal gradient and mineralizer concentration. RbBe 2BO 3F 2 crystals possessed a particularly good combination of SHG intensity, favorable crystal habit and fast growth rates. High quality crystals suitable for advanced deep-UV NLO studies were grown at rates of 0.11 mm/day on (0 0 1) and 0.12 mm/day perpendicular to (0 0 1).

Evidence for cis-trans isomerization of a double bond in the fatty acids of the psychrophilic bacterium Vibrio sp. strain ABE-1.

PubMed

Morita, N; Shibahara, A; Yamamoto, K; Shinkai, K; Kajimoto, G; Okuyama, H

1993-02-01

Vibrio sp. strain ABE-1 was grown in a medium that contained as its stable isotope tracer either [2,2-2H2]cis-9-hexadecenoic or [2,2-2H2]trans-9-hexadecenoic acid. Gas chromatographic-mass spectrometric analysis of the cis-9-hexadecenoic and trans-9-hexadecenoic acid fractions from the cells revealed the formation of an intracellularly isomerized 2,2-2H2-fatty acid which differed from the tracer only in the geometrical configuration of the double bond. This observation shows that cis-trans isomerization without a shift in double-bond position between these two geometric hexadecenoic acid isomers can occur in the cells.

Evidence for cis-trans isomerization of a double bond in the fatty acids of the psychrophilic bacterium Vibrio sp. strain ABE-1.

PubMed Central

Morita, N; Shibahara, A; Yamamoto, K; Shinkai, K; Kajimoto, G; Okuyama, H

1993-01-01

Vibrio sp. strain ABE-1 was grown in a medium that contained as its stable isotope tracer either [2,2-2H2]cis-9-hexadecenoic or [2,2-2H2]trans-9-hexadecenoic acid. Gas chromatographic-mass spectrometric analysis of the cis-9-hexadecenoic and trans-9-hexadecenoic acid fractions from the cells revealed the formation of an intracellularly isomerized 2,2-2H2-fatty acid which differed from the tracer only in the geometrical configuration of the double bond. This observation shows that cis-trans isomerization without a shift in double-bond position between these two geometric hexadecenoic acid isomers can occur in the cells. PMID:8423164

Adsorption of water and butanol in silicalite-1 film studied with in situ attenuated total reflectance-Fourier transform infrared spectroscopy.

PubMed

Farzaneh, Amirfarrokh; Zhou, Ming; Potapova, Elisaveta; Bacsik, Zoltán; Ohlin, Lindsay; Holmgren, Allan; Hedlund, Jonas; Grahn, Mattias

2015-05-05

Biobutanol produced by, e.g., acetone-butanol-ethanol (ABE) fermentation is a promising alternative to petroleum-based chemicals as, e.g., solvent and fuel. Recovery of butanol from dilute fermentation broths by hydrophobic membranes and adsorbents has been identified as a promising route. In this work, the adsorption of water and butanol vapor in a silicalite-1 film was studied using in situ attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy to better understand the adsorption properties of silicalite-1 membranes and adsorbents. Single-component adsorption isotherms were determined in the temperature range of 35-120 °C, and the Langmuir model was successfully fitted to the experimental data. The adsorption of butanol is very favorable compared to that of water. When the silicalite-1 film was exposed to a butanol/water vapor mixture with 15 mol % butanol (which is the vapor composition of an aqueous solution containing 2 wt % butanol, a typical concentration in an ABE fermentation broth, i.e., the composition of the gas obtained from gas stripping of an ABE broth) at 35 °C, the adsorption selectivity toward butanol was as high as 107. These results confirm that silicalite-1 quite selectively adsorbs hydrocarbons from vapor mixtures. To the best of our knowledge, this is the first comprehensive study on the adsorption of water and butanol in silicalite-1 from vapor phase.

Depositional and erosional architectures of gravelly braid bar formed by a flood in the Abe River, central Japan, inferred from a three-dimensional ground-penetrating radar analysis

NASA Astrophysics Data System (ADS)

Okazaki, Hiroko; Kwak, Youngjoo; Tamura, Toru

2015-07-01

We conducted a ground-penetrating radar (GPR) survey of gravelly braid bars in the Abe River, central Japan, to clarify the three-dimensional (3D) variations in their depositional facies under various geomorphologic conditions. In September 2011, a ten-year return-period flood in the study area reworked and deposited braid bars. After the flood, we surveyed three bars with different geomorphologies using a GPR system with a 250-MHz antenna and identified seven fundamental radar depositional facies: Inclined reflections (facies Ia and Ib), horizontal to subhorizontal reflections (facies IIa and IIb), discontinuous reflections (facies IIIa and IIIb), and facies assemblage with a large-scale channel-shaped lower boundary (facies IV). Combinations of these facies indicate bar formation processes: channel filling, lateral aggradation, and lateral and downstream accretion. In the Abe River, aerial photographs and airborne laser scanning data were obtained before and after the flood. The observed changes of the surface topography are consistent with the subsurface results seen in the GPR sections. This study demonstrated that the erosional and depositional architecture observed among bars with different channel styles was related to river width and represented depositional processes for high-sediment discharge. The quantitative characterizations of the sedimentary architecture will be useful for interpreting gravelly fluvial deposits in the rock record.

Geometric and Road Environmental Effects against Total Number of Traffic Accidents in Kendari

NASA Astrophysics Data System (ADS)

Kurdin, M. Akbar; Welendo, La; Annisa, Nur

2017-05-01

From the large number of traffic accidents that occurred, the carrying of Kendari as the biggest contributor to accidents in the Southeast. The number of accidents in Kendari row since 2011 was recorded at 18 accidents due to the influence of geometric road, in 2012 registered at 13 accident and in 2013 amounted to 6 accidents, with accident data because of the influence Geometric recorded for 3 consecutive years the biggest contributor to accidents because of the influence of geometric is Abeli districts. This study aimed to determine the road which common point of accident-prone (Black spot) in Kecamatan Abeli as accident-prone areas in Kendari, analyze the influence of geometric and road environment against accidents on roads in Kecamatan Abeli, provide alternative treatment based on the causes of accidents on the location of the accident-prone points (blackspot) to reduce the rate of traffic accidents. From the results of a study of 6 curve the accident-prone locations, that the curve I, II, and VI is the “Black Spot” influenced by the amount and condition of traffic accidents, while at the curve II, a traffic accident that occurred also be caused by unsafe geometric where the type of geometric should be changed from Spiral-Spiral type to Spiral-Circle-Spiral type. This indicates geometric effect on the number of accidents.

Enhancement of butanol tolerance and butanol yield in Clostridium acetobutylicum mutant NT642 obtained by nitrogen ion beam implantation.

PubMed

Liu, Xiao-Bo; Gu, Qiu-Ya; Yu, Xiao-Bin; Luo, Wei

2012-12-01

As a promising alternative biofuel, biobutanol can be produced through acetone/butanol/ethanol (ABE) fermentation. Currently, ABE fermentation is still a small-scale industry due to its low production and high input cost. Moreover, butanol toxicity to the Clostridium fermentation host limits the accumulation of butanol in the fermentation broth. The wild-type Clostridium acetobutylicum D64 can only produce about 13 g butanol/L and tolerates less than 2% (v/v) butanol. To improve the tolerance of C. acetobutylicum D64 for enhancing the production of butanol, nitrogen ion beam implantation was employed and finally five mutants with enhanced butanol tolerance were obtained. Among these, the most butanol tolerant mutant C. acetobutylicum NT642 can tolerate above 3% (v/v) butanol while the wide-type strain can only withstand 2% (v/v). In batch fermentation, the production of butanol and ABE yield of C. acetobutylicum NT642 was 15.4 g/L and 22.3 g/L, respectively, which were both higher than those of its parental strain and the other mutants using corn or cassava as substrate. Enhancing butanol tolerance is a great precondition for obtaining a hyper-yield producer. Nitrogen ion beam implantation could be a promising biotechnology to improve butanol tolerance and production of the host strain C. acetobutylicum.

Integrated, systems metabolic picture of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum.

PubMed

Liao, Chen; Seo, Seung-Oh; Celik, Venhar; Liu, Huaiwei; Kong, Wentao; Wang, Yi; Blaschek, Hans; Jin, Yong-Su; Lu, Ting

2015-07-07

Microbial metabolism involves complex, system-level processes implemented via the orchestration of metabolic reactions, gene regulation, and environmental cues. One canonical example of such processes is acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum, during which cells convert carbon sources to organic acids that are later reassimilated to produce solvents as a strategy for cellular survival. The complexity and systems nature of the process have been largely underappreciated, rendering challenges in understanding and optimizing solvent production. Here, we present a system-level computational framework for ABE fermentation that combines metabolic reactions, gene regulation, and environmental cues. We developed the framework by decomposing the entire system into three modules, building each module separately, and then assembling them back into an integrated system. During the model construction, a bottom-up approach was used to link molecular events at the single-cell level into the events at the population level. The integrated model was able to successfully reproduce ABE fermentations of the WT C. acetobutylicum (ATCC 824), as well as its mutants, using data obtained from our own experiments and from literature. Furthermore, the model confers successful predictions of the fermentations with various network perturbations across metabolic, genetic, and environmental aspects. From foundation to applications, the framework advances our understanding of complex clostridial metabolism and physiology and also facilitates the development of systems engineering strategies for the production of advanced biofuels.

Integrated, systems metabolic picture of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum

PubMed Central

Liao, Chen; Seo, Seung-Oh; Celik, Venhar; Liu, Huaiwei; Kong, Wentao; Wang, Yi; Blaschek, Hans; Jin, Yong-Su; Lu, Ting

2015-01-01

Microbial metabolism involves complex, system-level processes implemented via the orchestration of metabolic reactions, gene regulation, and environmental cues. One canonical example of such processes is acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum, during which cells convert carbon sources to organic acids that are later reassimilated to produce solvents as a strategy for cellular survival. The complexity and systems nature of the process have been largely underappreciated, rendering challenges in understanding and optimizing solvent production. Here, we present a system-level computational framework for ABE fermentation that combines metabolic reactions, gene regulation, and environmental cues. We developed the framework by decomposing the entire system into three modules, building each module separately, and then assembling them back into an integrated system. During the model construction, a bottom-up approach was used to link molecular events at the single-cell level into the events at the population level. The integrated model was able to successfully reproduce ABE fermentations of the WT C. acetobutylicum (ATCC 824), as well as its mutants, using data obtained from our own experiments and from literature. Furthermore, the model confers successful predictions of the fermentations with various network perturbations across metabolic, genetic, and environmental aspects. From foundation to applications, the framework advances our understanding of complex clostridial metabolism and physiology and also facilitates the development of systems engineering strategies for the production of advanced biofuels. PMID:26100881

A strategy for optical properties investigation in ABe2BO3F2 (A=K, Rb, Cs) using finite field methods

NASA Astrophysics Data System (ADS)

Mushahali, Hahaer; Mu, Baoxia; Wang, Qian; Mamat, Mamatrishat; Cao, Haibin; Yang, Guang; Jing, Qun

2018-07-01

The finite-field methods can be used to intuitively learn about the optical response and find out the atomic contributions to the birefringence and SHG tensors. In this paper, the linear and second-order nonlinear optical properties of ABe2BO3F2 family (A = K, Rb, Cs) compounds are investigated using the finite-field methods within different exchange-correlation functionals. The results show that the obtained birefringence and SHG tensors are in good agreement with the experimental values. The atomic contribution to the total birefringence was further investigated using the variation of the atomic charges, and the Born effective charges. The results show that the boron-oxygen groups give main contribution to the anisotropic birefringence.

Production of Butyric Acid and Butanol from Biomass

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ramey, David E.; Yang, Shang-Tian

Butanol replaced gasoline gallon for gallon in a 10,000 miles trip across the United States without the need to highly modify a ’92 Buick (your existing car today). Butanol can now be made for less than ethanol and yields more Btu’s from the same corn, making the plow to tire equation positive – more energy out than it takes to make it and Butanol is much safer. Butanol when substituted for gasoline gives better gas mileage and does not pollute as tested in 10 states. Butanol should now receive the same recognition as ethanol in U.S. legislation “ethanol/butanol”. There ismore » abundant plant biomass present as low-value agricultural commodities or processing wastes requiring proper disposal to avoid pollution problems. One example is in the corn refinery industry, which processes more than 13% of the ~9.5 billion bushels (~240 million metric tons) of corn annually produced in the U.S. to produce high-fructose-corn-syrup, dextrose, starch, and fuel alcohol, and generates more than 10 million metric tons of corn byproducts that are currently of limited use and pose significant environmental problems. The abundant inexpensive renewable resources as feedstock for fermentation, and recent advances in the fields of biotechnology and bioprocessing have resulted in a renewed interest in the fermentation production of chemicals and fuels, including n-butanol. The historic acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum is one of the oldest known industrial fermentations. It was ranked second only to ethanol fermentation by yeast in its scale of production, and is one of the largest biotechnological processes ever known. However, since the 1950's industrial ABE fermentation has declined continuously, and almost all butanol is now produced via petrochemical routes (Chemical Marketing Reporter, 1993). Butanol is an important industrial solvent and is a better fuel for replacing gasoline – gallon for gallon than ethanol. Current butanol prices as a chemical are at $3.00 per gallon – wholesaling in 55 gallon drums for $6.80, with a worldwide market of 1.4 billion gallon per year. The market demand is expected to increase dramatically since butanol can now be produced economically from low-cost biomass. Butanol’s application as a replacement for gasoline will outpace ethanol, biodiesel and hydrogen when its safety and simplicity of use are seen. Butanol’s application for the Department of Defense as a clean-safe replacement for batteries when used in conjunction with fuel cell technology is seen as an application for the future. Disposable canisters made of PLA that carry butanol to be reformed and used to generate electricity for computers, night vision and stealth equipment can be easily disposed of. In a typical ABE fermentation, butyric, propionic and acetic acids are produced first by C. acetobutylicum; the culture then undergoes a metabolic shift and solvents (butanol, acetone, and ethanol) are formed (Fond et al., 1985). In conventional ABE fermentations, the butanol yield from glucose is low, typically at ~15% (w/w) and rarely exceeds 25% (0.77–1.3 gallons per bushel corn respectfully). The production of butanol is also limited by severe product inhibition. Butanol at a concentration of 10 g/L can significantly inhibit cell growth and the fermentation. Consequently, butanol titers in conventional ABE fermentations are usually lower than 13 g/L. The low butanol yield and butanol concentration made butanol production from glucose by ABE fermentation uneconomical.« less

Virtual environments for the transfer of navigation skills in the blind: a comparison of directed instruction vs. video game based learning approaches.

PubMed

Connors, Erin C; Chrastil, Elizabeth R; Sánchez, Jaime; Merabet, Lotfi B

2014-01-01

For profoundly blind individuals, navigating in an unfamiliar building can represent a significant challenge. We investigated the use of an audio-based, virtual environment called Audio-based Environment Simulator (AbES) that can be explored for the purposes of learning the layout of an unfamiliar, complex indoor environment. Furthermore, we compared two modes of interaction with AbES. In one group, blind participants implicitly learned the layout of a target environment while playing an exploratory, goal-directed video game. By comparison, a second group was explicitly taught the same layout following a standard route and instructions provided by a sighted facilitator. As a control, a third group interacted with AbES while playing an exploratory, goal-directed video game however, the explored environment did not correspond to the target layout. Following interaction with AbES, a series of route navigation tasks were carried out in the virtual and physical building represented in the training environment to assess the transfer of acquired spatial information. We found that participants from both modes of interaction were able to transfer the spatial knowledge gained as indexed by their successful route navigation performance. This transfer was not apparent in the control participants. Most notably, the game-based learning strategy was also associated with enhanced performance when participants were required to find alternate routes and short cuts within the target building suggesting that a ludic-based training approach may provide for a more flexible mental representation of the environment. Furthermore, outcome comparisons between early and late blind individuals suggested that greater prior visual experience did not have a significant effect on overall navigation performance following training. Finally, performance did not appear to be associated with other factors of interest such as age, gender, and verbal memory recall. We conclude that the highly interactive and immersive exploration of the virtual environment greatly engages a blind user to develop skills akin to positive near transfer of learning. Learning through a game play strategy appears to confer certain behavioral advantages with respect to how spatial information is acquired and ultimately manipulated for navigation.

Virtual environments for the transfer of navigation skills in the blind: a comparison of directed instruction vs. video game based learning approaches

PubMed Central

Connors, Erin C.; Chrastil, Elizabeth R.; Sánchez, Jaime; Merabet, Lotfi B.

2014-01-01

For profoundly blind individuals, navigating in an unfamiliar building can represent a significant challenge. We investigated the use of an audio-based, virtual environment called Audio-based Environment Simulator (AbES) that can be explored for the purposes of learning the layout of an unfamiliar, complex indoor environment. Furthermore, we compared two modes of interaction with AbES. In one group, blind participants implicitly learned the layout of a target environment while playing an exploratory, goal-directed video game. By comparison, a second group was explicitly taught the same layout following a standard route and instructions provided by a sighted facilitator. As a control, a third group interacted with AbES while playing an exploratory, goal-directed video game however, the explored environment did not correspond to the target layout. Following interaction with AbES, a series of route navigation tasks were carried out in the virtual and physical building represented in the training environment to assess the transfer of acquired spatial information. We found that participants from both modes of interaction were able to transfer the spatial knowledge gained as indexed by their successful route navigation performance. This transfer was not apparent in the control participants. Most notably, the game-based learning strategy was also associated with enhanced performance when participants were required to find alternate routes and short cuts within the target building suggesting that a ludic-based training approach may provide for a more flexible mental representation of the environment. Furthermore, outcome comparisons between early and late blind individuals suggested that greater prior visual experience did not have a significant effect on overall navigation performance following training. Finally, performance did not appear to be associated with other factors of interest such as age, gender, and verbal memory recall. We conclude that the highly interactive and immersive exploration of the virtual environment greatly engages a blind user to develop skills akin to positive near transfer of learning. Learning through a game play strategy appears to confer certain behavioral advantages with respect to how spatial information is acquired and ultimately manipulated for navigation. PMID:24822044

Engineering Clostridium acetobutylicum for production of kerosene and diesel blendstock precursors.

PubMed

Bormann, Sebastian; Baer, Zachary C; Sreekumar, Sanil; Kuchenreuther, Jon M; Dean Toste, F; Blanch, Harvey W; Clark, Douglas S

2014-09-01

Processes for the biotechnological production of kerosene and diesel blendstocks are often economically unattractive due to low yields and product titers. Recently, Clostridium acetobutylicum fermentation products acetone, butanol, and ethanol (ABE) were shown to serve as precursors for catalytic upgrading to higher chain-length molecules that can be used as fuel substitutes. To produce suitable kerosene and diesel blendstocks, the butanol:acetone ratio of fermentation products needs to be increased to 2-2.5:1, while ethanol production is minimized. Here we show that the overexpression of selected proteins changes the ratio of ABE products relative to the wild type ATCC 824 strain. Overexpression of the native alcohol/aldehyde dehydrogenase (AAD) has been reported to primarily increase ethanol formation in C. acetobutylicum. We found that overexpression of the AAD(D485G) variant increased ethanol titers by 294%. Catalytic upgrading of the 824(aad(D485G)) ABE products resulted in a blend with nearly 50wt%≤C9 products, which are unsuitable for diesel. To selectively increase butanol production, C. beijerinckii aldehyde dehydrogenase and C. ljungdhalii butanol dehydrogenase were co-expressed (strain designate 824(Cb ald-Cl bdh)), which increased butanol titers by 27% to 16.9gL(-1) while acetone and ethanol titers remained essentially unaffected. The solvent ratio from 824(Cb ald-Cl bdh) resulted in more than 80wt% of catalysis products having a carbon chain length≥C11 which amounts to 9.8gL(-1) of products suitable as kerosene or diesel blendstock based on fermentation volume. To further increase solvent production, we investigated expression of both native and heterologous chaperones in C. acetobutylicum. Expression of a heat shock protein (HSP33) from Bacillus psychrosaccharolyticus increased the total solvent titer by 22%. Co-expression of HSP33 and aldehyde/butanol dehydrogenases further increased ABE formation as well as acetone and butanol yields. HSP33 was identified as the first heterologous chaperone that significantly increases solvent titers above wild type C. acetobutylicum levels, which can be combined with metabolic engineering to further increase solvent production. Copyright © 2014 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

Demographic analysis of dormancy and survival in the terrestrial orchid Cypripedium reginae

USGS Publications Warehouse

Kery, Marc; Gregg, Katharine B.

2004-01-01

1. We use capture-recapture models to estimate the fraction of dormant ramets, survival and state transition rates, and to identify factors affecting these rates, for the terrestrial orchid Cypripedium reginae. We studied two populations in West Virginia, USA, for 11 years and investigated relationships between grazing and demography. Abe Run's population was small, with moderate herbivory by deer and relatively constant population size. The population at Big Draft was of medium size, with heavy deer grazing, and a sharply declining number of flowering plants up to the spring before our study started, when the population was fenced. 2. We observed dormant episodes lasting from 1 to 4 years. At Abe Run and Big Draft, 32.5% and 7.4% of ramets, respectively, were dormant at least once during the study period for an average of 1.6 and 1.3 years, respectively. We estimated the annual fraction of ramets in the dormant state at 12.3% (95% CI 9.5-15.8%) at Abe Run and at 1.8% (95% CI 1.2-2.6%) at Big Draft. Transition rates between the dormant, vegetative and flowering life-states did not vary between years in either population. Most surviving ramets remained in the same state from one year to the next. Survival rates were constant at Abe Run (0.96, 95% CI 0.93-0.97), but varied between years at Big Draft (0.89-0.99, mean 0.95). 3. At Big Draft, we found neither a temporal trend in survival after cessation of grazing, nor relationships between survival and the number of spring frost days or cumulative precipitation during the current or the previous 12 months. However, analysis of precipitation on a 3-month basis revealed a positive relationship between survival and precipitation during the spring (March-May) of the previous year. 4. Relationship between climate and the population dynamics of orchids may have to be studied with a fine temporal resolution, and considering possible time lags. Capture-recapture modelling provides a comprehensive and flexible framework for demographic analysis of plants with dormancy.

Prospective and development of butanol as an advanced biofuel.

PubMed

Xue, Chuang; Zhao, Xin-Qing; Liu, Chen-Guang; Chen, Li-Jie; Bai, Feng-Wu

2013-12-01

Butanol has been acknowledged as an advanced biofuel, but its production through acetone-butanol-ethanol (ABE) fermentation by clostridia is still not economically competitive, due to low butanol yield and titer. In this article, update progress in butanol production is reviewed. Low price and sustainable feedstocks such as lignocellulosic residues and dedicated energy crops are needed for butanol production at large scale to save feedstock cost, but processes are more complicated, compared to those established for ABE fermentation from sugar- and starch-based feedstocks. While rational designs targeting individual genes, enzymes or pathways are effective for improving butanol yield, global and systems strategies are more reasonable for engineering strains with stress tolerance controlled by multigenes. Compared to solvent-producing clostridia, engineering heterologous species such as Escherichia coli and Saccharomyces cerevisiae with butanol pathway might be a solution for eliminating the formation of major byproducts acetone and ethanol so that butanol yield can be improved significantly. Although batch fermentation has been practiced for butanol production in industry, continuous operation is more productive for large scale production of butanol as a biofuel, but a single chemostat bioreactor cannot achieve this goal for the biphasic ABE fermentation, and tanks-in-series systems should be optimized for alternative feedstocks and new strains. Moreover, energy saving is limited for the distillation system, even total solvents in the fermentation broth are increased significantly, since solvents are distilled to ~40% by the beer stripper, and more than 95% water is removed with the stillage without phase change, even with conventional distillation systems, needless to say that advanced chemical engineering technologies can distil solvents up to ~90% with the beer stripper, and the multistage pressure columns can well balance energy consumption for solvent fraction. Indeed, an increase in butanol titer with ABE fermentation can significantly save energy consumption for medium sterilization and stillage treatment, since concentrated medium can be used, and consequently total mass flow with production systems can be reduced. As for various in situ butanol removal technologies, their energy efficiency, capital investment and contamination risk to the fermentation process need to be evaluated carefully. © 2013 Elsevier Inc. All rights reserved.

Fractionating the unitary notion of dissociation: disembodied but not embodied dissociative experiences are associated with exocentric perspective-taking

PubMed Central

Braithwaite, Jason J.; James, Kelly; Dewe, Hayley; Medford, Nick; Takahashi, Chie; Kessler, Klaus

2013-01-01

It has been argued that hallucinations which appear to involve shifts in egocentric perspective (e.g., the out-of-body experience, OBE) reflect specific biases in exocentric perspective-taking processes. Via a newly devised perspective-taking task, we examined whether such biases in perspective-taking were present in relation to specific dissociative anomalous body experiences (ABE) – namely the OBE. Participants also completed the Cambridge Depersonalization Scale (CDS; Sierra and Berrios, 2000) which provided measures of additional embodied ABE (unreality of self) and measures of derealization (unreality of surroundings). There were no reliable differences in the level of ABE, emotional numbing, and anomalies in sensory recall reported between the OBE and control group as measured by the corresponding CDS subscales. In contrast, the OBE group did provide significantly elevated measures of derealization (“alienation from surroundings” CDS subscale) relative to the control group. At the same time we also found that the OBE group was significantly more efficient at completing all aspects of the perspective-taking task relative to controls. Collectively, the current findings support fractionating the typically unitary notion of dissociation by proposing a distinction between embodied dissociative experiences and disembodied dissociative experiences – with only the latter being associated with exocentric perspective-taking mechanisms. Our findings – obtained with an ecologically valid task and a homogeneous OBE group – also call for a re-evaluation of the relationship between OBEs and perspective-taking in terms of facilitated disembodied experiences. PMID:24198776

A quantitative metabolomics study of high sodium response in Clostridium acetobutylicum ATCC 824 acetone-butanol-ethanol (ABE) fermentation

PubMed Central

Zhao, Xinhe; Condruz, Stefan; Chen, Jingkui; Jolicoeur, Mario

2016-01-01

Hemicellulose hydrolysates, sugar-rich feedstocks used in biobutanol refinery, are normally obtained by adding sodium hydroxide in the hydrolyze process. However, the resulting high sodium concentration in the hydrolysate inhibits ABE (acetone-butanol-ethanol) fermentation, and thus limits the use of these low-cost feedstocks. We have thus studied the effect of high sodium on the metabolic behavior of Clostridium acetobutyricum ATCC 824, with xylose as the carbon source. At a threshold sodium concentration of 200 mM, a decrease of the maximum cell dry weight (−19.50 ± 0.85%) and of ABE yield (−35.14 ± 3.50% acetone, −33.37 ± 0.74% butanol, −22.95 ± 1.81% ethanol) were observed compared to control culture. However, solvents specific productivities were not affected by supplementing sodium. The main effects of high sodium on cell metabolism were observed in acidogenesis, during which we observed the accumulation of ATP and NADH, and the inhibition of the pentose phosphate (PPP) and the glycolytic pathways with up to 80.73 ± 1.47% and 68.84 ± 3.42% decrease of the associated metabolic intermediates, respectively. However, the NADP+-to-NADPH ratio was constant for the whole culture duration, a phenomenon explaining the robustness of solvents specific productivities. Therefore, high sodium, which inhibited biomass growth through coordinated metabolic effects, interestingly triggered cell robustness on solvents specific productivity. PMID:27321153

New species of cynipid inquilines of the genus Saphonecrus (Hymenoptera: Cynipidae: Synergini) from the Eastern Palaearctic, with a re-appraisal of known species world-wide.

PubMed

Schwéger, Szabina; Melika, George; Tang, Chang-Ti; Yang, Man-Miao; Stone, Graham N; Nicholls, James A; Sinclair, Frazer; Hearn, Jack; Bozsó, Miklós; Pénzes, Zsolt

2015-12-08

Fifteen new species of cynipid inquilines, Saphonecrus chinensis Tang & Schwéger, S. gilvus Melika & Schwéger, S. globosus Schwéger & Tang, S. leleyi Melika & Schwéger, S. lithocarpii Schwéger & Melika, S. longinuxi Schwéger & Melika, S. morii Schwéger & Tang, S. nantoui Tang, Schwéger & Melika, S. nichollsi Schwéger & Melika, S. pachylomai Schwéger, Tang & Melika, S. robustus Schwéger & Melika, S. saliciniai Melika, Tang & Schwéger, S. shanzhukui Melika & Tang, S. symbioticus Melika & Schwéger, and S. taitungi Schwéger, Tang & Melika, from the Eastern Palaearctic are described. Descriptions, diagnoses, biology, and host associations for the new species, and a key to Palaearctic Saphonecrus species are given. All new taxa form distinct units as demonstrated by the molecular phylogenetic analyses of Palaearctic Saphonecrus species. The status of some earlier described Saphonecrus species is discussed also. The Synergini genus Lithonecrus Nieves-Aldrey & Butterill, 2014 is synonymized with Lithosaphonecrus Tang, Melika & Bozsó, 2013. Three Saphonecrus species are transferred to Synergus: Synergus brevis (Weld) comb. nova, Synergus hupingshanensis (Liu, Yang & Zhu) comb. nova, and Synergus yukawai (Wachi, Ide & Abe) comb. nova. Synophrus vietnamensis Abe, Ide, Konishi & Ueno is transferred to Lithosaphonecrus: Lithosaphonecrus vietnamensis Abe, Ide, Konishi & Ueno), comb. nova. The current number of valid Saphonecrus species worldwide is 36.

Endothelial cell palmitoylproteomics identifies novel lipid modified targets and potential substrates for protein acyl transferases

PubMed Central

Marin, Ethan P.; Derakhshan, Behrad; Lam, TuKiet T.; Davalos, Alberto; Sessa, William C.

2012-01-01

Rationale Protein S-palmitoylation is the post-translational attachment of a saturated 16-carbon palmitic acid to a cysteine side chain via a thioester bond. Palmitoylation can affect protein localization, trafficking, stability, and function. The extent and roles of palmitoylation in endothelial cell (EC) biology is not well understood, in part due to technological limits on palmitoylprotein detection. Objective To develop a method using acyl-biotinyl exchange (ABE) technology coupled with mass spectrometry to globally isolate and identify palmitoylproteins in EC. Methods and Results More than 150 putative palmitoyl proteins were identified in EC using ABE and mass spectrometry. Among the novel palmitoylproteins identified is superoxide dismutase 1 (SOD1), an intensively studied enzyme that protects all cells from oxidative damage. Mutation of cysteine 6 prevents palmitoylation, leads to reduction in SOD1 activity in vivo and in vitro, and inhibits nuclear localization, thereby supporting a functional role for SOD1 palmitoylation. Moreover, we used ABE to search for substrates of particular protein acyl transferases in EC. We found that palmitoylation of the cell adhesion protein PECAM1 is dependent on the protein acyl transferase ZDHHC21. We show that knockdown of ZDHHC21 leads to reduced levels of PECAM1 at the cell surface. Conclusions Our data demonstrate the utility of EC palmitoylproteomics to reveal new insights into the role of this important post-translational lipid modification in EC biology. PMID:22496122

Techno-economic analysis of extraction-based separation systems for acetone, butanol, and ethanol recovery and purification.

PubMed

Grisales Díaz, Víctor Hugo; Olivar Tost, Gerard

2017-01-01

Dual extraction, high-temperature extraction, mixture extraction, and oleyl alcohol extraction have been proposed in the literature for acetone, butanol, and ethanol (ABE) production. However, energy and economic evaluation under similar assumptions of extraction-based separation systems are necessary. Hence, the new process proposed in this work, direct steam distillation (DSD), for regeneration of high-boiling extractants was compared with several extraction-based separation systems. The evaluation was performed under similar assumptions through simulation in Aspen Plus V7.3 ® software. Two end distillation systems (number of non-ideal stages between 70 and 80) were studied. Heat integration and vacuum operation of some units were proposed reducing the energy requirements. Energy requirement of hybrid processes, substrate concentration of 200 g/l, was between 6.4 and 8.3 MJ-fuel/kg-ABE. The minimum energy requirements of extraction-based separation systems, feeding a water concentration in the substrate equivalent to extractant selectivity, and ideal assumptions were between 2.6 and 3.5 MJ-fuel/kg-ABE, respectively. The efficiencies of recovery systems for baseline case and ideal evaluation were 0.53-0.57 and 0.81-0.84, respectively. The main advantages of DSD were the operation of the regeneration column at atmospheric pressure, the utilization of low-pressure steam, and the low energy requirements of preheating. The in situ recovery processes, DSD, and mixture extraction with conventional regeneration were the approaches with the lowest energy requirements and total annualized costs.

The role of art education in adult prisons: The Western Australian experience

NASA Astrophysics Data System (ADS)

Giles, Margaret; Paris, Lisa; Whale, Jacqui

2016-12-01

Incarceration costs are high; in Australia, for example, each prisoner costs an average of AUD 115,000 per year. Other countries are also feeling the fiscal pinch of high incarceration costs, and a number of jurisdictions are now closing some of their prisons. Most prison costs are non-discretionary (accommodation, meals, etc.). But some of the costs relate to discretionary activities, services and facilities (including schooling). In terms of correctional education, many prison managers try to invest any meagre correctional education resources available to them in those classes and courses which have proven to have the best results, such as improved labour market outcomes and reduced recidivism, minimising subsequent re-imprisonment. Course offers for prisoner-students include vocational training, adult basic education (ABE) and art studies. The two-tiered question this paper asks is: do art classes and courses produce these measurable outcomes and, if not, are there other reasons why they should continue to be funded? Addressing these issues, the authors argue that (1) these measurable outcomes are too narrow and do not reflect the complex but less quantifiable benefits to the individual and the community of studying art in prison, and (2) better measures of all impacts of art studies in prisons are needed, including qualitative and humanitarian aspects.

Solvent (acetone-butanol: ab) production

USDA-ARS?s Scientific Manuscript database

This article describes production of butanol [acetone-butanol-ethanol, (also called AB or ABE or solvent)] by fermentation using both traditional and current technologies. AB production from agricultural commodities, such as corn and molasses, was an important historical fermentation. Unfortunately,...

Strategies to Overcome Negative Reading Habits of ABE Participants: A Case Study.

ERIC Educational Resources Information Center

Stanfel, Jane E.

1996-01-01

The author theorizes that her students, single-parent welfare recipients, develop negative reading habits to camouflage low literacy. She describes instructional strategies that fail to help and methods she found effective. (SK)

Genetics Home Reference: Pyle disease

MedlinePlus

... of print] Citation on PubMed Haraguchi R, Kitazawa R, Mori K, Tachibana R, Kiyonari H, Imai Y, Abe T, ... K, Kiviranta R, Solban N, Liu J, Brommage R, Boduroglu K, Bonafé L, Campos-Xavier B, Dikoglu E, Eastell ...

Brefeldin A is an estrogenic, Erk1/2-activating component in the extract of Agaricus blazei mycelia.

PubMed

Dong, Sijun; Furutani, Yoshiyuki; Kimura, Sadao; Zhu, Yun; Kawabata, Kazutaka; Furutani, Michiko; Nishikawa, Toshio; Tanaka, Takeshi; Masaki, Tomoh; Matsuoka, Rumiko; Kiyama, Ryoiti

2013-01-09

We purified an Erk1/2-activating component in Agaricus blazei and identified it as brefeldin A (BFA). The extract of A. blazei mycelia (ABE) previously showed an estrogenic gene-expression profile and positive effects in patients with cardiovascular symptoms. Here, we demonstrate that BFA has estrogenic activity in reporter gene assays and stimulates an estrogen-receptor pathway revealed by activation of Erk1/2, although BFA had no growth-stimulating activity in breast cancer MCF-7 cells. The presence of estrogenic activity without any explicit growth-stimulating effect is unique to BFA, and such components are termed here "silent estrogens". To test this hypothesis, we examined the target-gene transcription and signaling pathways induced by BFA. Furthermore, BFA was found in the mycelium but not fruiting body of A. blazei, suggesting the potential use of ABE for therapeutics and its supplementary use in traditional medicines and functional foods.

Shushaku Endo (1923–1996): his tuberculosis and his writings

PubMed Central

Sharma, O P

2006-01-01

Shushaku Endo, one of the finest 20th century Japanese novelists, is not as well known in the West as some of his contemporary Japanese writers including, Yukio Mishima, Yasunari Kawabata, Kobo Abe, and Kenzaburo Oe. Mishima deals with turmoil of adolescence laced with surrealistic historical romance; Abe conjures strange, evocative images in the Kafkaesque tradition; Kawabata writes about loneliness and desolation; and Oe creates imaginary themes of life and myth that entwine and portray human predicaments. Kawabata and Oe both received Nobel Prizes in Literature. Endo's writing is different. He confronts problems pertaining to faith, morality, and individual responsibility. In his early years Endo struggled as a loner, an outsider, and a misfit. This pattern continued to a large extent into his adult life, most of which was consumed with fighting his life threatening tuberculosis. The agonies of his illness intensified his talent for exploring the complexity of human relationships. PMID:16517794

Shushaku Endo (1923-1996): his tuberculosis and his writings.

PubMed

Sharma, O P

2006-03-01

Shushaku Endo, one of the finest 20th century Japanese novelists, is not as well known in the West as some of his contemporary Japanese writers including, Yukio Mishima, Yasunari Kawabata, Kobo Abe, and Kenzaburo Oe. Mishima deals with turmoil of adolescence laced with surrealistic historical romance; Abe conjures strange, evocative images in the Kafkaesque tradition; Kawabata writes about loneliness and desolation; and Oe creates imaginary themes of life and myth that entwine and portray human predicaments. Kawabata and Oe both received Nobel Prizes in Literature. Endo's writing is different. He confronts problems pertaining to faith, morality, and individual responsibility. In his early years Endo struggled as a loner, an outsider, and a misfit. This pattern continued to a large extent into his adult life, most of which was consumed with fighting his life threatening tuberculosis. The agonies of his illness intensified his talent for exploring the complexity of human relationships.

Effect of chemical pretreatments on corn stalk bagasse as immobilizing carrier of Clostridium acetobutylicum in the performance of a fermentation-pervaporation coupled system.

PubMed

Cai, Di; Li, Ping; Chen, Changjing; Wang, Yong; Hu, Song; Cui, Caixia; Qin, Peiyong; Tan, Tianwei

2016-11-01

In this study, different pretreatment methods were evaluated for modified the corn stalk bagasse and further used the pretreated bagasse as immobilized carrier in acetone-butanol-ethanol fermentation process. Structural changes of the bagasses pretreated by different methods were analyzed by Fourier transform infrared, crystallinity index and scanning pictures by electron microscope. And the performances of batch fermentation using the corn stalk based carriers were evaluated. Results indicated that the highest ABE concentration of 23.86g/L was achieved using NaOH pretreated carrier in batch fermentation. Immobilized fermentation-pervaporation integration process was further carried out. The integration process showed long-term stability with 225-394g/L of ABE solvents on the permeate side of pervaporation membrane. This novel integration process was found to be an efficient method for biobutanol production. Copyright © 2016 Elsevier Ltd. All rights reserved.

Monte Carlo methods and their analysis for Coulomb collisions in multicomponent plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bobylev, A.V., E-mail: alexander.bobylev@kau.se; Potapenko, I.F., E-mail: firena@yandex.ru

2013-08-01

Highlights: •A general approach to Monte Carlo methods for multicomponent plasmas is proposed. •We show numerical tests for the two-component (electrons and ions) case. •An optimal choice of parameters for speeding up the computations is discussed. •A rigorous estimate of the error of approximation is proved. -- Abstract: A general approach to Monte Carlo methods for Coulomb collisions is proposed. Its key idea is an approximation of Landau–Fokker–Planck equations by Boltzmann equations of quasi-Maxwellian kind. It means that the total collision frequency for the corresponding Boltzmann equation does not depend on the velocities. This allows to make the simulation processmore » very simple since the collision pairs can be chosen arbitrarily, without restriction. It is shown that this approach includes the well-known methods of Takizuka and Abe (1977) [12] and Nanbu (1997) as particular cases, and generalizes the approach of Bobylev and Nanbu (2000). The numerical scheme of this paper is simpler than the schemes by Takizuka and Abe [12] and by Nanbu. We derive it for the general case of multicomponent plasmas and show some numerical tests for the two-component (electrons and ions) case. An optimal choice of parameters for speeding up the computations is also discussed. It is also proved that the order of approximation is not worse than O(√(ε)), where ε is a parameter of approximation being equivalent to the time step Δt in earlier methods. A similar estimate is obtained for the methods of Takizuka and Abe and Nanbu.« less

Non-traditional Aharonov-Bohm effects in condensed matter

DOE Office of Scientific and Technical Information (OSTI.GOV)

Krive, I.V.; Rozhavsky, A.S.

1992-05-10

In 1959, Aharonov and Bohm proposed an elegant experiment demonstrating observability of electromagnetic potentials (or, which is the same, the non-locality of the wave function of charged particles) in quantum mechanics. This paper discusses the Aharonov-Bohm effect, based on the fundamental principles of quantum theory, as the superposition principles, the quantum character of motion of particles and locality of the interaction of a charge with an electromagnetic potential L{sub int} = j{sub {mu}}A{sup {mu}}. It is thus no wonder that the Aharonov-Bohm's paper aroused much dispute which is still ongoing. Originally, the Aharonov-Bohm effect (ABE) means the dependence of themore » interference pattern on the magnetic fluid flux {phi} in a Gendaken experiment on a coherent electron beam in the field of an infinitely thin solenoid. Later, however, it became common to refer to the Aharonov-Bohm phenomenon wherever the characteristics of systems under study appear to depend on the flux {phi} in the absence of electric and magnetic fields. In this sense, it was highly interesting to analyze the ABE in condensed media (the many-particle Aharonov-Bohm effect), in particular to study the dependence of the thermodynamic and kinetic characteristics, e.g., of metal on the flux. Such a problem was first discussed by Byers and Yang who formulated the general theorems related to the ABE in conducting condensed media. The next important step was the work of Kulik who formulated a concrete model and calculated the flux-dependent contribution to the metal free energy and provided a first clear formulation of the requirements to reveal.« less

First Discovery and Investigation of a High-Temperature Hydrothermal Vent Field on the Ultra- Slow Spreading Southwest Indian Ridge

NASA Astrophysics Data System (ADS)

Tao, C.; Lin, J.; Guo, S.; Chen, Y. J.; Wu, G.; Han, X.; German, C. R.; Yoerger, D. R.; Zhu, J.; Zhou, N.; Su, X.; Baker, E. T.; Party, S.

2007-12-01

Two recent cruises on board the Chinese research vessel Dayang Yihao have successfully investigated the first active hydrothermal vent field to be located along the ultraslow spreading Southwest Indian Ridge (SWIR) and collected hydrothermal sulfide deposit samples. The newly discovered hydrothermal vent field is located on the western end of a magmatically robust spreading segment immediately west of the Gallieni transform fault. Preliminary evidence of strong turbidity anomalies was first measured during a Nov. 2005 cruise on board Dayang Yihao (InterRidge News, vol. 15, pp. 33-34, 2006). Color video footages of the seafloor in the vent-field area were first obtained by a deep-towed video camera in February 2007 during DY115-19 Leg 1, when significant water column turbidity anomalies, noticeable temperature anomalies and methane anomalies were also measured. The vent field was then precisely located, mapped, and photographed in great detail in February- March 2007 during the DY115-19 Leg 2, using the autonomous underwater vehicle ABE of the Woods Hole Oceanographic Institution. A high-resolution bathymetric map, more than 5,000 near-bottom color photos, and several types of water column data were all obtained during three phases of ABE dives. Within the approximately 120-m-long by 100-m-wide hydrothermal field, three groups of active high-temperature vents were identified and color images of black smokers and associated biological communities were obtained from ABE, flying 5 m above the seafloor. Hydrothermal sulfide deposits were then successfully obtained using a TV-guided grab.

First Discovery and Investigation of a High-Temperature Hydrothermal Vent Field on the Ultra- Slow Spreading Southwest Indian Ridge

NASA Astrophysics Data System (ADS)

Tao, C.; Lin, J.; Guo, S.; Chen, Y. J.; Wu, G.; Han, X.; German, C. R.; Yoerger, D. R.; Zhu, J.; Zhou, N.; Su, X.; Baker, E. T.; Party, S.

2004-12-01

Two recent cruises on board the Chinese research vessel Dayang Yihao have successfully investigated the first active hydrothermal vent field to be located along the ultraslow spreading Southwest Indian Ridge (SWIR) and collected hydrothermal sulfide deposit samples. The newly discovered hydrothermal vent field is located on the western end of a magmatically robust spreading segment immediately west of the Gallieni transform fault. Preliminary evidence of strong turbidity anomalies was first measured during a Nov. 2005 cruise on board Dayang Yihao (InterRidge News, vol. 15, pp. 33-34, 2006). Color video footages of the seafloor in the vent-field area were first obtained by a deep-towed video camera in February 2007 during DY115-19 Leg 1, when significant water column turbidity anomalies, noticeable temperature anomalies and methane anomalies were also measured. The vent field was then precisely located, mapped, and photographed in great detail in February- March 2007 during the DY115-19 Leg 2, using the autonomous underwater vehicle ABE of the Woods Hole Oceanographic Institution. A high-resolution bathymetric map, more than 5,000 near-bottom color photos, and several types of water column data were all obtained during three phases of ABE dives. Within the approximately 120-m-long by 100-m-wide hydrothermal field, three groups of active high-temperature vents were identified and color images of black smokers and associated biological communities were obtained from ABE, flying 5 m above the seafloor. Hydrothermal sulfide deposits were then successfully obtained using a TV-guided grab.

Binge eating, trauma, and suicide attempt in community adults with major depressive disorder.

PubMed

Baek, Ji Hyun; Kim, Kiwon; Hong, Jin Pyo; Cho, Maeng Je; Fava, Maurizio; Mischoulon, David; Chang, Sung Man; Kim, Ji Yeon; Cho, Hana; Jeon, Hong Jin

2018-01-01

Eating disorders comorbid with depression are an established risk factor for suicide. In this study, we aimed to determine the effects of binge eating (BE) symptoms on suicidality and related clinical characteristics in major depressive disorder (MDD). A total of 817 community participants with MDD were included. We compared two groups (with and without lifetime BE symptoms). The MDD with BE group was subdivided into a frequent BE (FBE) subgroup (BE symptoms greater than twice weekly) and any BE (ABE) subgroup (BE symptoms greater than twice weekly). The MDD with BE group comprised 142 (17.38%) patients. The FBE and ABE subgroups comprised 75 (9.18%) and 67 (8.20%) patients, respectively. Comorbid alcohol use disorder, anxiety disorder, post-traumatic stress disorder (PTSD) and history of suicide attempt were significantly more frequent in the MDD with BE group than MDD without BE group. Sexual trauma was also reported more frequently in MDD with BE group. No significant differences were observed between the ABE and FBE subgroups. Multivariate logistic regression revealed an association of suicide attempt with BE symptoms and sexual trauma. Structural equation modeling showed that sexual trauma increased BE (β = 0.337, P <0.001) together with alcohol use (β = 0.185, P <0.001) and anxiety (β = 0.299, p<0.001), which in turn increased suicide attempt (β = 0.087, p = 0.011). BE symptoms were associated with suicide attempt in MDD after adjusting for other factors associated with suicidality. BE symptoms also moderated an association between suicide attempt and sexual trauma.

Cholesterol, inflammasomes, and atherogenesis

USDA-ARS?s Scientific Manuscript database

Plasma cholesterol levels have been strongly associated with atherogenesis, underscoring the role of lipid metabolism in defining cardiovascular disease risk. However, atherosclerotic plaque is highly dynamic and contains elements of both the innate and adaptive immune system that respond to the abe...

Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4.

PubMed

Herman, Nicolaus A; Li, Jeffrey; Bedi, Ripika; Turchi, Barbara; Liu, Xiaoji; Miller, Michael J; Zhang, Wenjun

2017-01-15

While a majority of academic studies concerning acetone, butanol, and ethanol (ABE) production by Clostridium have focused on Clostridium acetobutylicum, other members of this genus have proven to be effective industrial workhorses despite the inability to perform genetic manipulations on many of these strains. To further improve the industrial performance of these strains in areas such as substrate usage, solvent production, and end product versatility, transformation methods and genetic tools are needed to overcome the genetic intractability displayed by these species. In this study, we present the development of a high-efficiency transformation method for the industrial butanol hyperproducer Clostridium saccharoperbutylacetonicum strain N1-4 (HMT) ATCC 27021. Following initial failures, we found that the key to creating a successful transformation method was the identification of three distinct colony morphologies (types S, R, and I), which displayed significant differences in transformability. Working with the readily transformable type I cells (transformation efficiency, 1.1 × 10 6 CFU/μg DNA), we performed targeted gene deletions in C. saccharoperbutylacetonicum N1-4 using a homologous recombination-mediated allelic exchange method. Using plasmid-based gene overexpression and targeted knockouts of key genes in the native acetone-butanol-ethanol (ABE) metabolic pathway, we successfully implemented rational metabolic engineering strategies, yielding in the best case an engineered strain (Clostridium saccharoperbutylacetonicum strain N1-4/pWIS13) displaying an 18% increase in butanol titers and 30% increase in total ABE titer (0.35 g ABE/g sucrose) in batch fermentations. Additionally, two engineered strains overexpressing aldehyde/alcohol dehydrogenases (encoded by adh11 and adh5) displayed 8.5- and 11.8-fold increases (respectively) in batch ethanol production. This paper presents the first steps toward advanced genetic engineering of the industrial butanol producer Clostridium saccharoperbutylacetonicum strain N1-4 (HMT). In addition to providing an efficient method for introducing foreign DNA into this species, we demonstrate successful rational engineering for increasing solvent production. Examples of future applications of this work include metabolic engineering for improving desirable industrial traits of this species and heterologous gene expression for expanding the end product profile to include high-value fuels and chemicals. Copyright © 2016 American Society for Microbiology.

Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4

PubMed Central

Herman, Nicolaus A.; Li, Jeffrey; Bedi, Ripika; Turchi, Barbara; Liu, Xiaoji

2016-01-01

ABSTRACT While a majority of academic studies concerning acetone, butanol, and ethanol (ABE) production by Clostridium have focused on Clostridium acetobutylicum, other members of this genus have proven to be effective industrial workhorses despite the inability to perform genetic manipulations on many of these strains. To further improve the industrial performance of these strains in areas such as substrate usage, solvent production, and end product versatility, transformation methods and genetic tools are needed to overcome the genetic intractability displayed by these species. In this study, we present the development of a high-efficiency transformation method for the industrial butanol hyperproducer Clostridium saccharoperbutylacetonicum strain N1-4 (HMT) ATCC 27021. Following initial failures, we found that the key to creating a successful transformation method was the identification of three distinct colony morphologies (types S, R, and I), which displayed significant differences in transformability. Working with the readily transformable type I cells (transformation efficiency, 1.1 × 106 CFU/μg DNA), we performed targeted gene deletions in C. saccharoperbutylacetonicum N1-4 using a homologous recombination-mediated allelic exchange method. Using plasmid-based gene overexpression and targeted knockouts of key genes in the native acetone-butanol-ethanol (ABE) metabolic pathway, we successfully implemented rational metabolic engineering strategies, yielding in the best case an engineered strain (Clostridium saccharoperbutylacetonicum strain N1-4/pWIS13) displaying an 18% increase in butanol titers and 30% increase in total ABE titer (0.35 g ABE/g sucrose) in batch fermentations. Additionally, two engineered strains overexpressing aldehyde/alcohol dehydrogenases (encoded by adh11 and adh5) displayed 8.5- and 11.8-fold increases (respectively) in batch ethanol production. IMPORTANCE This paper presents the first steps toward advanced genetic engineering of the industrial butanol producer Clostridium saccharoperbutylacetonicum strain N1-4 (HMT). In addition to providing an efficient method for introducing foreign DNA into this species, we demonstrate successful rational engineering for increasing solvent production. Examples of future applications of this work include metabolic engineering for improving desirable industrial traits of this species and heterologous gene expression for expanding the end product profile to include high-value fuels and chemicals. PMID:27836845

Butanol production by fermentation: efficient bioreactors

USDA-ARS?s Scientific Manuscript database

Energy security, environmental concerns, and business opportunities in the emerging bio-economy have generated strong interest in the production of n-butanol by fermentation. Acetone butanol ethanol (ABE or solvent) batch fermentation process is product limiting because butanol even at low concentra...

76 FR 55388 - Radio Broadcasting Services; AM or FM Proposals To Change the Community of License.

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-07

... Broadcasters, Inc., Station NEW, Facility ID 183343, BMPH-20110804ABE, from Big Sur, CA, to Molus, CA; Winton...., Washington, DC 20554 or electronically via the Media Bureau's Consolidated Data Base System, http...

77 FR 58624 - ABE Fairmont, LLC-Acquisition and Operation Exemption-Fillmore Western Railway Company

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-21

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Contribution of efflux to colistin heteroresistance in a multidrug resistant Acinetobacter baumannii clinical isolate.

PubMed

Machado, Diana; Antunes, Jéssica; Simões, Ana; Perdigão, João; Couto, Isabel; McCusker, Matthew; Martins, Marta; Portugal, Isabel; Pacheco, Teresa; Batista, Judite; Toscano, Cristina; Viveiros, Miguel

2018-06-01

The mechanisms underlying colistin heteroresistance in Acinetobacter baumannii are not fully understood. Here, we investigated the role of efflux in colistin-heteroresistant populations of a multidrug-resistant (MDR) A. baumannii clinical isolate. Three colistin-resistant A. baumannii strain variants isolated from the same clinical sample were studied for the presence of heteroresistance to colistin by drug susceptibility testing, genotyping and drug resistance target mutation analysis. The existence of active efflux was studied by synergism assays with efflux inhibitors, real-time efflux activity measurements and analysis of the mRNA transcriptional levels of selected efflux pump genes in response to colistin. All of the strain variants belong to the ST218, clonal complex 92, international clonal lineage II. Different colistin susceptibility levels were observed among the three strain variants, indicating that colistin-heteroresistant subpopulations were being selected upon exposure to colistin. No mutations were found in the genes lpxACD and pmrAB, which are associated with colistin resistance. The results showed the existence of synergistic interactions between efflux inhibitors and colistin and ethidium bromide. Real-time efflux assays demonstrated that the three strain variants had increased efflux activity that could be inhibited in the presence of the inhibitors. The efflux pump genes adeB, adeJ, adeG, craA, amvA, abeS and abeM were found to be overexpressed in the strain variants in response to colistin exposure. This study shows that efflux activity contributes to colistin heteroresistance in an MDR A. baumannii clinical isolate. The use of efflux inhibitors as adjuvants of the therapy can resensitize A. baumannii to colistin and prevent the emergence of drug resistance.

Cognitive and affective benefits of combination therapy with galantamine plus cognitive rehabilitation for Alzheimer's disease.

PubMed

Tokuchi, Ryo; Hishikawa, Nozomi; Matsuzono, Kosuke; Takao, Yoshiki; Wakutani, Yosuke; Sato, Kota; Kono, Syoichiro; Ohta, Yasuyuki; Deguchi, Kentaro; Yamashita, Toru; Abe, Koji

2016-04-01

The aim of the present study was to compare the effects of a galantamine only therapy and a combination therapy with galantamine plus ambulatory cognitive rehabilitation for Alzheimer's disease patients. For this retrospective cohort study, we enrolled 86 patients with Alzheimer's disease, dividing them into two groups - a galantamine only group (group G, n = 45) and a combination with galantamine plus ambulatory rehabilitation group (group G + R, n = 41). The present cognitive rehabilitation included a set of physical therapy, occupational therapy and speech therapy for 1-2 h once or twice a week. We compared the Mini-Mental State Examination and Frontal Assessment Battery for cognitive assessment, and Geriatric Depression Scale, Apathy Scale, and Abe's Behavioral and Psychological Symptoms of Dementia score for affective assessment in two groups over 6 months. The baseline Mini-Mental State Examination score was 20.2 and 18.7 in groups G and G + R, respectively. Other baseline data (Frontal Assessment Battery, Geriatric Depression Scale, Apathy Scale, and Abe's Behavioral and Psychological Symptoms of Dementia) were not different between the two groups. Although group G kept all the scores stable until 6 months of the treatment, the Apathy Scale score showed a significant improvement in group G + R as early as 3 months, followed by the Mini-Mental State Examination and Frontal Assessment Battery improvements at 6 months (*P = 0.04 and *P = 0.02, respectively). The Geriatric Depression Scale and Abe's Behavioral and Psychological Symptoms of Dementia did not show any changes. The combination therapy of galantamine plus ambulatory cognitive rehabilitation showed a superior benefit both on cognitive and affective functions than galantamine only therapy in Alzheimer's disease patients. © 2015 Japan Geriatrics Society.

Life-cycle assessment of corn-based butanol as a potential transportation fuel.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu, M.; Wang, M.; Liu, J.

2007-12-31

Butanol produced from bio-sources (such as corn) could have attractive properties as a transportation fuel. Production of butanol through a fermentation process called acetone-butanol-ethanol (ABE) has been the focus of increasing research and development efforts. Advances in ABE process development in recent years have led to drastic increases in ABE productivity and yields, making butanol production worthy of evaluation for use in motor vehicles. Consequently, chemical/fuel industries have announced their intention to produce butanol from bio-based materials. The purpose of this study is to estimate the potential life-cycle energy and emission effects associated with using bio-butanol as a transportation fuel.more » The study employs a well-to-wheels analysis tool--the Greenhouse Gases, Regulated Emissions and Energy Use in Transportation (GREET) model developed at Argonne National Laboratory--and the Aspen Plus{reg_sign} model developed by AspenTech. The study describes the butanol production from corn, including grain processing, fermentation, gas stripping, distillation, and adsorption for products separation. The Aspen{reg_sign} results that we obtained for the corn-to-butanol production process provide the basis for GREET modeling to estimate life-cycle energy use and greenhouse gas emissions. The GREET model was expanded to simulate the bio-butanol life cycle, from agricultural chemical production to butanol use in motor vehicles. We then compared the results for bio-butanol with those of conventional gasoline. We also analyzed the bio-acetone that is coproduced with bio-butanol as an alternative to petroleum-based acetone. Our study shows that, while the use of corn-based butanol achieves energy benefits and reduces greenhouse gas emissions, the results are affected by the methods used to treat the acetone that is co-produced in butanol plants.« less

Design and characterization of hirulogs: A novel class of bivalent peptide inhibitors of thrombin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Maraganore, J.M.; Bourdon, P.; Jablonski, J.

1990-07-31

A novel class of synthetic peptides has been designed that inhibit the thrombin catalytic site and exhibit specificity for the anion-binding exosite (ABE) of {alpha}-thrombin. These peptides, called hirulogs, consist of (i) an active-site specificity sequence with a restricted Arg-Pro scissile bond, (ii) a polymeric linker of glycyl residues from 6 to 18 {angstrom} in length, and (iii) an ABE recognition sequence such as that in the hirudin C-terminus. Hirulog-1 ((D-Phe)-Pro-Arg-Pro-(Gly){sub 4}-Asn-Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Tyr-Leu) inhibits the thrombin-catalyzed hydrolysis of a tripeptide p-nitroanilide substrate with K{sub i} = 2.3 nM. In contrast, the synthetic C-terminal hirudin peptide S-Hir{sub 53-64}, which binds to themore » thrombin ABE, blocked the fibrinogen clotting activity of the enzyme with K{sub i} = 144 nM but failed to inhibit the hydrolysis of p-nitroanilide substrates at concentrations as high as 1 mM. Hirulog-1, but not S-Hir{sub 53-64}, was found to inhibit the incorporation of ({sup 14}C)diisopropyl fluorophosphate in thrombin. Hirulog-1 appears specific for thrombin as it lacks inhibitory activities toward human factor Xa, human plasmin, and bovine trypsin at inhibitor:enzyme concentrations 3 orders of magnitude higher than those required to inhibit thrombin. The optimal inhibitory activity of hirulog-1 depends upon all three components of its structure. Comparison of anticoagulant activities of hirulog-1, hirudin, and S-Hir{sub 53-64} showed that the synthetic hirulog-1 is 2-fold more potent than hirudin and 100-fold more active than S-Hir{sub 53-64} in increasing the activated partial thromboplastin time of normal human plasma.« less

Divided attention can enhance early-phase memory encoding: the attentional boost effect and study trial duration.

PubMed

Mulligan, Neil W; Spataro, Pietro

2015-07-01

Divided attention during encoding typically produces marked reductions in later memory. The attentional boost effect (ABE) is a surprising variation on this phenomenon. In this paradigm, each study stimulus (e.g., a word) is presented along with a target or a distractor (e.g., different colored circles) in a detection task. Later memory is better for stimuli co-occurring with targets. The present experiments indicate that the ABE arises during an early phase of memory encoding that involves initial stimulus perception and comprehension rather than at a later phase entailing controlled, elaborative rehearsal. Experiment 1 demonstrated that the ABE was robust at a short study duration (700 ms) and did not increase with increasing study trial durations (1,500 ms and 4,000 ms). Furthermore, the target condition is boosted to the level of memory performance in a full-attention condition for the short duration but not the long duration. Both results followed from the early-phase account. This account also predicts that for very short study times (limiting the influence of late-phase controlled encoding and thus minimizing the usual negative effect of divided attention), the target condition will produce better memory than will the full-attention condition. Experiment 2 used a study time of 400 ms and found that words presented with targets lead to greater recognition accuracy than do either words presented with distractors or words in the full-attention condition. Consistent with the early-phase account, a divided attention condition actually produced superior memory than did the full-attention condition, a very unusual but theoretically predicted result. (c) 2015 APA, all rights reserved.

Metabolic engineering of Clostridium acetobutylicum for the enhanced production of isopropanol-butanol-ethanol fuel mixture.

PubMed

Jang, Yu-Sin; Malaviya, Alok; Lee, Joungmin; Im, Jung Ae; Lee, Sang Yup; Lee, Julia; Eom, Moon-Ho; Cho, Jung-Hee; Seung, Do Young

2013-01-01

Butanol is considered as a superior biofuel, which is conventionally produced by clostridial acetone-butanol-ethanol (ABE) fermentation. Among ABE, only butanol and ethanol can be used as fuel alternatives. Coproduction of acetone thus causes lower yield of fuel alcohols. Thus, this study aimed at developing an improved Clostridium acetobutylicum strain possessing enhanced fuel alcohol production capability. For this, we previously developed a hyper ABE producing BKM19 strain was further engineered to convert acetone into isopropanol. The BKM19 strain was transformed with the plasmid pIPA100 containing the sadh (primary/secondary alcohol dehydrogenase) and hydG (putative electron transfer protein) genes from the Clostridium beijerinckii NRRL B593 cloned under the control of the thiolase promoter. The resulting BKM19 (pIPA100) strain produced 27.9 g/l isopropanol-butanol-ethanol (IBE) as a fuel alcohols with negligible amount of acetone (0.4 g/l) from 97.8 g/l glucose in lab-scale (2 l) batch fermentation. Thus, this metabolically engineered strain was able to produce 99% of total solvent produced as fuel alcohols. The scalability and stability of BKM19 (pIPA100) were evaluated at 200 l pilot-scale fermentation, which showed that the fuel alcohol yield could be improved to 0.37 g/g as compared to 0.29 g/g obtained at lab-scale fermentation, while attaining a similar titer. To the best of our knowledge, this is the highest titer of IBE achieved and the first report on the large scale fermentation of C. acetobutylicum for IBE production. © 2013 American Institute of Chemical Engineers.

Measurements of I-129 in meteorites and lunar rock by tandem accelerator mass spectrometry

NASA Technical Reports Server (NTRS)

Nizhiizumi, K.; Arnold, J. R.; Elmore, D.; Gove, H. E.; Honda, M.

1983-01-01

Precise measurements of the half-life of I-129 in three different meteorites and one lunar surface rock are reported. The meteorite source of I-129 was produced by cosmic ray secondary neutron reactions on Te, while the source in lunar materials in spallation on barium and rare earth elements. The Abee, Allende, and Dhajala meteorites were examined, together with the lunar rock 14310. Details of the process used to extract the iodine are provided. The Abee and Allende samples exhibited a production of 0.5 atom/min per gm of Te from the (n,2n) reaction and 0.05 atom/min/gm for the (n,gamma) reaction. The I-129 is concluded to be a viable tool for long-lived cosmogenic nuclide studies. Further work to extend the data to include the constancy of the cosmic ray flux, the meteorite bombardment history, and the cosmic exposure age dating by means of the I-129 and Xe-129 method is indicated.

The Attentional Boost Effect with Verbal Materials

ERIC Educational Resources Information Center

Mulligan, Neil W.; Spataro, Pietro; Picklesimer, Milton

2014-01-01

Study stimuli presented at the same time as unrelated targets in a detection task are better remembered than stimuli presented with distractors. This attentional boost effect (ABE) has been found with pictorial (Swallow & Jiang, 2010) and more recently verbal materials (Spataro, Mulligan, & Rossi-Arnaud, 2013). The present experiments…

Far-field tsunami magnitude determined from ocean-bottom pressure gauge data around Japan

NASA Astrophysics Data System (ADS)

Baba, T.; Hirata, K.; Kaneda, Y.

2003-12-01

\\hspace*{3mm}Tsunami magnitude is the most fundamental parameter to scale tsunamigenic earthquakes. According to Abe (1979), the tsunami magnitude, Mt, is empirically related to the crest to trough amplitude, H, of the far-field tsunami wave in meters (Mt = logH + 9.1). Here we investigate the far-field tsunami magnitude using ocean-bottom pressure gauge data. The recent ocean-bottom pressure measurements provide more precise tsunami data with a high signal-to-noise ratio. \\hspace*{3mm}Japan Marine Science and Technology Center is monitoring ocean bottom pressure fluctuations using two submarine cables of depths of 1500 - 2400 m. These geophysical observatory systems are located off Cape Muroto, Southwest Japan, and off Hokkaido, Northern Japan. The ocean-bottom pressure data recorded with the Muroto and Hokkaido systems have been collected continuously since March, 1997 and October, 1999, respectively. \\hspace*{3mm}Over the period from March 1997 to June 2003, we have observed four far-field tsunami signals, generated by earthquakes, on ocean-bottom pressure records. These far-field tsunamis were generated by the 1998 Papua New Guinea eq. (Mw 7.0), 1999 Vanuatu eq. (Mw 7.2), 2001 Peru eq. (Mw 8.4) and 2002 Papua New Guinea eq. (Mw 7.6). Maximum amplitude of about 30 mm was recorded by the tsunami from the 2001 Peru earthquake. \\hspace*{3mm}Direct application of the Abe's empirical relation to ocean-bottom pressure gauge data underestimates tsunami magnitudes by about an order of magnitude. This is because the Abe's empirical relation was derived only from tsunami amplitudes with coastal tide gauges where tsunami is amplified by the shoaling of topography and the reflection at the coastline. However, these effects do not work for offshore tsunami in deep oceans. In general, amplification due to shoaling near the coastline is governed by the Green's Law, in which the tsunami amplitude is proportional to h-1/4, where h is the water depth. Wave amplitude also is doubled by reflection at the fixed edge (coastline). Hence, we introduce a water-depth term and a reflection coefficient of 2 in the original Abe_fs empirical relation to correct tsunami amplitude for open oceans and obtain Mt = log(2H/h-1/4) + 9.1, where h is the depth of the ocean bottom pressure gage. The modified empirical relation produces tsunami magnitudes close to those determined using tide gauges.

Melanges Pedagogiques (Pedagogical Mixture), 1983.

ERIC Educational Resources Information Center

Melanges Pedagogiques, 1983

1983-01-01

The 1983 issue of the journal on second language teaching and learning contains six articles in French. These include the following: "E.A.O.: Expression avec ordinateur (E.A.O.: Computer-Aided Expression)" (Daniele Abe, Michele Cembalo); "Ou suis-je? De la relation apprenant/environnement (Where Am I? On the Learner/Environment…

Using Queer Theory to Explore Lesbian College Students' Multiple Dimensions of Identity

ERIC Educational Resources Information Center

Abes, Elisa S.; Kasch, David

2007-01-01

By introducing queer theory to Abes and Jones's (2004) constructivist narrative inquiry of lesbian college students' perceptions of their multiple identities, we retell the developmental narrative of one participant's negotiation of her sexuality, religion, gender, and social class. This queer retelling of a developmental story shows how…

Radical Conversations: Part Two--Cultivating Social-Constructivist Learning Methods in ABE Classrooms

ERIC Educational Resources Information Center

Muth, Bill; Kiser, Madeline

2008-01-01

In many U.S. prisons an overuse of individualized instruction silences literacy learners and reinforces oppressive notions about what knowledge is and whose knowledge counts. In these classrooms, methods that invite learners to tap their background knowledge, reflect on their worlds, and dialogue with others to construct meaning--commonplace in…

Butanol production from concentrated lactose/whey permeate: Use of pervaporation membrane to recover and concentrate product

USDA-ARS?s Scientific Manuscript database

In these studies butanol (acetone butanol ethanol, or ABE) was produced from concentrated lactose/whey permeate containing 211 gL-1 lactose. Fermentation of such a highly concentrated lactose solution was possible due to simultaneous product removal using a pervaporation membrane. In this system a p...

Baseline Experiment for Active Control of Structural Vibrations

DTIC Science & Technology

1989-12-01

like to thank iy wife , Lynette, for marrying an engineer. Although she didn’t always understand what ! was U ii Avallab1tY CodeA . Dlst Special doing...actuators. The structural dynamics , haker 0 used for the modified ABE provided sufficient and predictable control force, and there are "off the shelf

Butanol productivity enhancers in wheat straw hydrolyzate: employing potential of enhanced reaction rate

USDA-ARS?s Scientific Manuscript database

Butanol production by fermentation is gaining momentum due to increased prices of fossil fuels. This biofuel is a major product of acetone-butanol-ethanol (ABE) fermentation that can be produced from hydrolyzed agricultural residues and/or corn. A control glucose (60 g/L) based batch fermentation us...

Novel developments in butanol fermentation: Microbial genetics to agricultural substrates, process technology, and downstream processing

USDA-ARS?s Scientific Manuscript database

Butanol is the major product of acetone-butanol-ethanol (ABE; ratio 3:6:1) fermentation. It can be produced from various carbohydrates such as glucose, corn, molasses, and whey permeate (a by-product of the dairy industry) using microbial strains such as Clostridium beijerinckii and/or C. acetobuty...

Agricultural residues and energy crops as potentially economical and novel substrates for microbial production of butanol (a biofuel)

USDA-ARS?s Scientific Manuscript database

This review describes production of acetone butanol ethanol (ABE) from a variety of agricultural residues and energy crops employing biochemical or fermentation processes. A number of organisms are available for this bioconversion including Clostridium beijerinckii P260, C. beijerinckii BA101, C. a...

Perceived Experiences of Anti-Bisexual Prejudice: Instrument Development and Evaluation

ERIC Educational Resources Information Center

Brewster, Melanie E.; Moradi, Bonnie

2010-01-01

This research describes the development and psychometric evaluation of the Anti-Bisexual Experiences Scale (ABES). Items were developed on the basis of prior literature, revised on the basis of expert feedback, and submitted to psychometric evaluation. Exploratory factor analysis of data from 350 bisexual participants yielded 3 factors of reported…

Global Journal of Computer Science and Technology. Volume 9, Issue 5 (Ver. 2.0)

ERIC Educational Resources Information Center

Dixit, R. K.

2010-01-01

This is a special issue published in version 1.0 of "Global Journal of Computer Science and Technology." Articles in this issue include: (1) [Theta] Scheme (Orthogonal Milstein Scheme), a Better Numerical Approximation for Multi-dimensional SDEs (Klaus Schmitz Abe); (2) Input Data Processing Techniques in Intrusion Detection…

Mutant strain of C. acetobutylicum and process for making butanol

DOEpatents

Jain, Mahendra K.; Beacom, Daniel; Datta, Rathin

1993-01-01

A biologically pure asporogenic mutant of Clostridium acetobutylicum is produced by growing sporogenic C. acetobutylicum ATCC 4259 and treating the parent strain with ethane methane sulfonate. The mutant which as been designated C. acetobutylicum ATCC 55025 is useful in an improved ABE fermentation process, and produces high concentrations of butanol and total solvents.

Improving Butanol Fermentation To Enter the Advanced Biofuel Market

PubMed Central

Tracy, Bryan P.

2012-01-01

ABSTRACT 1-Butanol is a large-volume, intermediate chemical with favorable physical and chemical properties for blending with or directly substituting for gasoline. The per-volume value of butanol, as a chemical, is sufficient for investing into the recommercialization of the classical acetone-butanol-ethanol (ABE) (E. M. Green, Curr. Opin. Biotechnol. 22:337–343, 2011) fermentation process. Furthermore, with modest improvements in three areas of the ABE process, operating costs can be sufficiently decreased to make butanol an economically viable advanced biofuel. The three areas of greatest interest are (i) maximizing yields of butanol on any particular substrate, (ii) expanding substrate utilization capabilities of the host microorganism, and (iii) reducing the energy consumption of the overall production process, in particular the separation and purification operations. In their study in the September/October 2012 issue of mBio, Jang et al. [mBio 3(5):e00314-12, 2012] describe a comprehensive study on driving glucose metabolism in Clostridium acetobutylicum to the production of butanol. Moreover, they execute a metabolic engineering strategy to achieve the highest yet reported yields of butanol on glucose. PMID:23232720

Genome Editing Tools in Plants

PubMed Central

Mohanta, Tapan Kumar; Bashir, Tufail; Hashem, Abeer; Bae, Hanhong

2017-01-01

Genome editing tools have the potential to change the genomic architecture of a genome at precise locations, with desired accuracy. These tools have been efficiently used for trait discovery and for the generation of plants with high crop yields and resistance to biotic and abiotic stresses. Due to complex genomic architecture, it is challenging to edit all of the genes/genomes using a particular genome editing tool. Therefore, to overcome this challenging task, several genome editing tools have been developed to facilitate efficient genome editing. Some of the major genome editing tools used to edit plant genomes are: Homologous recombination (HR), zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), pentatricopeptide repeat proteins (PPRs), the CRISPR/Cas9 system, RNA interference (RNAi), cisgenesis, and intragenesis. In addition, site-directed sequence editing and oligonucleotide-directed mutagenesis have the potential to edit the genome at the single-nucleotide level. Recently, adenine base editors (ABEs) have been developed to mutate A-T base pairs to G-C base pairs. ABEs use deoxyadeninedeaminase (TadA) with catalytically impaired Cas9 nickase to mutate A-T base pairs to G-C base pairs. PMID:29257124

Simultaneous glucose and xylose uptake by an acetone/butanol/ethanol producing laboratory Clostridium beijerinckii strain SE-2.

PubMed

Zhang, Jie; Zhu, Wen; Xu, Haipeng; Li, Yan; Hua, Dongliang; Jin, Fuqiang; Gao, Mintian; Zhang, Xiaodong

2016-04-01

Most butanol-producing strains of Clostridium prefer glucose over xylose, leading to a slower butanol production from lignocellulose hydrolysates. It is therefore beneficial to find and use a strain that can simultaneously use both glucose and xylose. Clostridium beijerinckii SE-2 strain assimilated glucose and xylose simultaneously and produced ABE (acetone/butanol/ethanol). The classic diauxic growth behavior was not seen. Similar rates of sugar consumption (4.44 mM glucose h(-1) and 6.66 mM xylose h(-1)) were observed suggesting this strain could use either glucose or xylose as the substrate and it has a similar capability to degrade these two sugars. With different initial glucose:xylose ratios, glucose and xylose were consumed simultaneously at rates roughly proportional to their individual concentrations in the medium, leading to complete utilization of both sugars at the same time. ABE production profiles were similar on different substrates. Transcriptional studies on the effect of glucose and xylose supplementation, however, suggests a clear glucose inhibition on xylose metabolism-related genes is still present.

Effect of dilute alkaline pretreatment on the conversion of different parts of corn stalk to fermentable sugars and its application in acetone-butanol-ethanol fermentation.

PubMed

Cai, Di; Li, Ping; Luo, Zhangfeng; Qin, Peiyong; Chen, Changjing; Wang, Yong; Wang, Zheng; Tan, Tianwei

2016-07-01

To investigate the effect of dilute alkaline pretreatment on different parts of biomass, corn stalk was separated into flower, leaf, cob, husk and stem, which were treated by NaOH in range of temperature and chemical loading. The NaOH-pretreated solid was then enzymatic hydrolysis and used as the substrate for batch acetone-butanol-ethanol (ABE) fermentation. The results demonstrated the five parts of corn stalk could be used as potential feedstock separately, with vivid performances in solvents production. Under the optimized conditions towards high product titer, 7.5g/L, 7.6g/L, 9.4g/L, 7g/L and 7.6g/L of butanol was obtained in the fermentation broth of flower, leaf, cob, husk and stem hydrolysate, respectively. Under the optimized conditions towards high product yield, 143.7g/kg, 126.3g/kg, 169.1g/kg, 107.7g/kg and 116.4g/kg of ABE solvent were generated, respectively. Copyright © 2016 Elsevier Ltd. All rights reserved.

Flow cytometry analysis of Clostridium beijerinckii NRRL B-598 populations exhibiting different phenotypes induced by changes in cultivation conditions.

PubMed

Branska, Barbora; Pechacova, Zora; Kolek, Jan; Vasylkivska, Maryna; Patakova, Petra

2018-01-01

Biobutanol production by clostridia via the acetone-butanol-ethanol (ABE) pathway is a promising future technology in bioenergetics , but identifying key regulatory mechanisms for this pathway is essential in order to construct industrially relevant strains with high tolerance and productivity. We have applied flow cytometric analysis to C. beijerinckii NRRL B-598 and carried out comparative screening of physiological changes in terms of viability under different cultivation conditions to determine its dependence on particular stages of the life cycle and the concentration of butanol. Dual staining by propidium iodide (PI) and carboxyfluorescein diacetate (CFDA) provided separation of cells into four subpopulations with different abilities to take up PI and cleave CFDA, reflecting different physiological states. The development of a staining pattern during ABE fermentation showed an apparent decline in viability, starting at the pH shift and onset of solventogenesis, although an appreciable proportion of cells continued to proliferate. This was observed for sporulating as well as non-sporulating phenotypes at low solvent concentrations, suggesting that the increase in percentage of inactive cells was not a result of solvent toxicity or a transition from vegetative to sporulating stages. Additionally, the sporulating phenotype was challenged with butanol and cultivation with a lower starting pH was performed; in both these experiments similar trends were obtained-viability declined after the pH breakpoint, independent of the actual butanol concentration in the medium. Production characteristics of both sporulating and non-sporulating phenotypes were comparable, showing that in C. beijerinckii NRRL B-598, solventogenesis was not conditional on sporulation. We have shown that the decline in C. beijerinckii NRRL B-598 culture viability during ABE fermentation was not only the result of accumulated toxic metabolites, but might also be associated with a special survival strategy triggered by pH change.

In situ hydrogen, acetone, butanol, ethanol and microdiesel production by Clostridium acetobutylicum ATCC 824 from oleaginous fungal biomass.

PubMed

Hassan, Elhagag Ahmed; Abd-Alla, Mohamed Hemida; Bagy, Magdy Mohamed Khalil; Morsy, Fatthy Mohamed

2015-08-01

An in situ batch fermentation technique was employed for biohydrogen, acetone, butanol, ethanol and microdiesel production from oleaginous fungal biomass using the anaerobic fermentative bacterium Clostridium acetobutylicum ATCC 824. Oleaginous fungal Cunninghamella echinulata biomass which has ability to accumulate up to 71% cellular lipid was used as the substrate carbon source. The maximum cumulative hydrogen by C. acetobutylicum ATCC 824 from crude C. echinulata biomass was 260 ml H2 l(-1), hydrogen production efficiency was 0.32 mol H2 mole(-1) glucose and the hydrogen production rate was 5.2 ml H2 h(-1). Subsequently, the produced acids (acetic and butyric acids) during acidogenesis phase are re-utilized by ABE-producing clostridia and converted into acetone, butanol, and ethanol. The total ABE produced by C. acetobutylicum ATCC 824 during batch fermentation was 3.6 g l(-1) from crude fungal biomass including acetone (1.05 g l(-1)), butanol (2.19 g l(-1)) and ethanol (0.36 g l(-1)). C. acetobutylicum ATCC 824 has ability to produce lipolytic enzymes with a specific activity 5.59 U/mg protein to hydrolyze ester containing substrates. The lipolytic potential of C. acetobutylicum ATCC 824 was used as a biocatalyst for a lipase transesterification process using the produced ethanol from ABE fermentation for microdiesel production. The fatty acid ethyl esters (microdiesel) generated from the lipase transesterification of crude C. echinulata dry mass was analyzed by GC/MS as 15.4% of total FAEEs. The gross energy content of biohydrogen, acetone, butanol, ethanol and biodiesel generated through C. acetobutylicum fermentation from crude C. echinulata dry mass was 3113.14 kJ mol(-1). These results suggest a possibility of integrating biohydrogen, acetone, butanol and ethanol production technology by C. acetobutylicum with microdiesel production from crude C. echinulata dry mass and therefore improve the feasibility and commercialization of bioenergy production. Copyright © 2015 Elsevier Ltd. All rights reserved.

ERG/AKR1C3/AR Constitutes a Feed-Forward Loop for AR Signaling in Prostate Cancer Cells.

PubMed

Powell, Katelyn; Semaan, Louie; Conley-LaComb, M Katie; Asangani, Irfan; Wu, Yi-Mi; Ginsburg, Kevin B; Williams, Julia; Squire, Jeremy A; Maddipati, Krishna R; Cher, Michael L; Chinni, Sreenivasa R

2015-06-01

Intratumoral androgen synthesis in prostate cancer contributes to the development of castration-resistant prostate cancer (CRPC). Several enzymes responsible for androgen biosynthesis have been shown to be overexpressed in CRPC, thus contributing to CRPC in a castrated environment. The TMPRSS2-ERG transcription factor has been shown to be present in primary prostate cancer tumors as well as CRPC tumors. We hypothesize that TMPRSS2-ERG fusions regulate androgen biosynthetic enzyme (ABE) gene expression and the production of androgens, which contributes to the development of CRPC. We used a panel of assays, including lentivirus transduction, gene expression, chromatin immunoprecipitation and sequencing, liquid chromatography-mass spectrometric quantitation, immunocytochemistry, immunohistochemistry, and bioinformatics analysis of gene microarray databases, to determine ERG regulation of androgen synthesis. We found that ERG regulated the expression of the ABE AKR1C3 in prostate cancer cells via direct binding to the AKR1C3 gene. Knockdown of ERG resulted in reduced AKR1C3 expression, which caused a reduction in both DHT synthesis and PSA expression in VCaP prostate cancer cells treated with 5α-androstanedione (5α-Adione), a DHT precursor metabolite. Immunohistochemical staining revealed that ERG was coexpressed with AKR1C3 in prostate cancer tissue samples. These data suggest that AKR1C3 catalyzes the biochemical reduction of 5α-Adione to DHT in prostate cancer cells, and that ERG regulates this step through upregulation of AKR1C3 expression. Elucidation of ERG regulation of ABEs in CRPC may help to stratify TMPRSS2-ERG fusion-positive prostate cancer patients in the clinic for anti-androgen receptor-driven therapies; and AKR1C3 may serve as a valuable therapeutic target in the treatment of CRPC. ©2015 American Association for Cancer Research.

Diverse Narrative Structures in Contemporary Picturebooks: Opportunities for Children's Meaning-Making

ERIC Educational Resources Information Center

Pantaleo, Sylvia; Sipe, Lawrence R.

2012-01-01

In this article, the authors situate their discussion of diverse narrative structures in the context of oral storytelling practices and postmodern picturebooks. They focus on three contemporary picturebooks: "Don't Read This Book!" (Lewis, 2009), "Abe Lincoln Crosses a Creek: A Tall Thin Tale" (Hopkins, 2008), and "The Stinky Cheese Man and Other…

Cellulosic butanol biofuel production from sweet sorghum bagasse (SSB): Impact of hot water pretreatment and solid loadings on fermentation employing Clostridium beijerinckii P260

USDA-ARS?s Scientific Manuscript database

A novel butanol fermentation process was developed in which sweet sorghum bagasse (SSB) was pretreated using liquid hot water (LHW) pretreatment technique followed by enzymatic hydrolysis and butanol (acetone butanol ethanol; ABE) fermentation. A pretreatment temperature of 200 deg C resulted in the...

Analysis of a Digital Technique for Frequency Transposition of Speech.

DTIC Science & Technology

1985-09-01

scaled excitation function drives the vocal tract model. In a phone interview with James Kaiser of Bell Laboratories, he mentioned that current thinking...is processed using the Fast Fourier Transform (FFT) and then low pass filtered if desired. mAbe (Pb) FFT LPF- nih ~a s5ee.. S. 4Nrf#Nr Flow Chart for

Effect of Fuel Properties on Injection Characteristics of Four Different Diesel Injection Systems.

DTIC Science & Technology

1985-12-01

Effects on Combustion in Direct Injection Diesel Engines," SAE Technical Paper Series, No. 850108, International Congress and Exposi - tion, Detroit, MI...NIEMEYER) I AMXSY-CR 1 DEFENSE STANDARDIZATION OFFICE ABE SYNR R ATTN DR MILER 1 ABERDEEN PROVING GROUND MDATTN: DR S ILLE I 10-06 . 2 1005-5006 5203

Literacy Skill Differences between Adult Native English and Native Spanish Speakers

ERIC Educational Resources Information Center

Herman, Julia; Cote, Nicole Gilbert; Reilly, Lenore; Binder, Katherine S.

2013-01-01

The goal of this study was to compare the literacy skills of adult native English and native Spanish ABE speakers. Participants were 169 native English speakers and 124 native Spanish speakers recruited from five prior research projects. The results showed that the native Spanish speakers were less skilled on morphology and passage comprehension…

Reconceptualizing the Model of Multiple Dimensions of Identity: The Role of Meaning-Making Capacity in the Construction of Multiple Identities

ERIC Educational Resources Information Center

Abes, Elisa S.; Jones, Susan R.; McEwen, Marylu K.

2007-01-01

We reconceptualize Jones and McEwen's (2000) model of multiple dimensions of identity by incorporating meaning making, based on the results of Abes and Jones's (2004) study of lesbian college students. Narratives of three students who utilize different orders of Kegan's (1994) meaning making (formulaic, transitional, and foundational, as described…

Process integration for simultaneous saccharification, fermentation, and recovery (SSFR): Production of butanol from corn stover using Clostridium beijerinckii P260

USDA-ARS?s Scientific Manuscript database

A simultaneous saccharification, fermentation, and recovery (SSFR) process was developed for production of acetone butanol ethanol (AB or ABE), of which butanol is the main product, from corn stover employing Clostridium beijerinckii P260. Of the 86 gL^-1^ corn stover, over 97% of the sugars were r...

In Defense of Japan

DTIC Science & Technology

2018-01-03

national elections. The party majority gives Abe the potential to formally amend Article 9 of the Japanese constitution to authorize the Japanese Self ...Pacific. Japanese Constitution; Article 9; The United States and Japan Bi-lateral defense treaty; Collective Self Defense; Pacifism; Japanese Self ...involvement in collective self -defense (CSD). Factors leading up to this decision are a rapidly evolving strategic and political environment in

Divided Attention Can Enhance Early-Phase Memory Encoding: The Attentional Boost Effect and Study Trial Duration

ERIC Educational Resources Information Center

Mulligan, Neil W.; Spataro, Pietro

2015-01-01

Divided attention during encoding typically produces marked reductions in later memory. The attentional boost effect (ABE) is a surprising variation on this phenomenon. In this paradigm, each study stimulus (e.g., a word) is presented along with a target or a distractor (e.g., different colored circles) in a detection task. Later memory is better…

The Planning of In-Service Workshops: A Seminar (Atlanta, Feb. 14-17, 1971).

ERIC Educational Resources Information Center

Southern Regional Education Board, Atlanta, GA.

This document is a summation of a workshop designed to be a planning experience for ABE training personnel. It also contains a sampling of model training designs developed by the participants for inservice work. The object of the seminar was to provide an overview of relevant techniques and procedures for developing and implementing short-term…

Mitotic Mysteries: The Case of HP1.

PubMed

Higgins, Jonathan M G; Prendergast, Lisa

2016-03-07

The role of Heterochromatin Protein-1 (HP1) during mitosis has been controversial. Two recent studies in Science and Developmental Cell, from Tanno et al. (2015) and Abe et al. (2016), suggest that the means of HP1 localization and its function at inner centromeres are altered in cancer cells with chromosomal instability. Copyright © 2016 Elsevier Inc. All rights reserved.

More Misbehavior of Organisms: A Psi Chi Lecture by Marian and Robert Bailey

ERIC Educational Resources Information Center

Bihm, Elson M.; Gillaspy, J. Arthur, Jr.; Abbott, Hannah J.; Lammers, William J.

2010-01-01

In 1992, Dr. Marian Breland Bailey, assisted by her husband Robert E. Bailey, gave the following presentation at the Psi Chi Banquet of the University of Central Arkansas. She and her first husband, Keller Breland, were students of B. F. Skinner and established Animal Behavior Enterprises (ABE) in 1947 and the IQ Zoo in 1955. Unknown to many…

On JALT 95: Curriculum and Evaluation. Proceedings of the JALT International Conference on Language Teaching/Learning (22nd, Nagoya, Japan, November 1995). Section Two: Curriculum Design.

ERIC Educational Resources Information Center

Harrison, Ian; And Others

Texts of conference papers and summaries of colloquia on second language curriculum design are presented, including: "Competency Assessment in Curriculum Renewal" (summary of session with Ian Harrison, Francis Johnson, Christopher Candlin, Anthony Green, David Nunan, Charles Smith); "The Evolving of a Curriculum" (Hiroshi Abe,…

On the putative essential discreteness of q-generalized entropies

NASA Astrophysics Data System (ADS)

Plastino, A.; Rocca, M. C.

2017-12-01

It has been argued in Abe (2010), entitled Essential discreteness in generalized thermostatistics with non-logarithmic entropy, that ;continuous Hamiltonian systems with long-range interactions and the so-called q-Gaussian momentum distributions are seen to be outside the scope of non-extensive statistical mechanics;. The arguments are clever and appealing. We show here that, however, some mathematical subtleties render them unconvincing.

Development of an audio-based virtual gaming environment to assist with navigation skills in the blind.

PubMed

Connors, Erin C; Yazzolino, Lindsay A; Sánchez, Jaime; Merabet, Lotfi B

2013-03-27

Audio-based Environment Simulator (AbES) is virtual environment software designed to improve real world navigation skills in the blind. Using only audio based cues and set within the context of a video game metaphor, users gather relevant spatial information regarding a building's layout. This allows the user to develop an accurate spatial cognitive map of a large-scale three-dimensional space that can be manipulated for the purposes of a real indoor navigation task. After game play, participants are then assessed on their ability to navigate within the target physical building represented in the game. Preliminary results suggest that early blind users were able to acquire relevant information regarding the spatial layout of a previously unfamiliar building as indexed by their performance on a series of navigation tasks. These tasks included path finding through the virtual and physical building, as well as a series of drop off tasks. We find that the immersive and highly interactive nature of the AbES software appears to greatly engage the blind user to actively explore the virtual environment. Applications of this approach may extend to larger populations of visually impaired individuals.

Structures of asparagine-linked oligosaccharides from hen egg-yolk antibody (IgY). Occurrence of unusual glucosylated oligo-mannose type oligosaccharides in a mature glycoprotein.

PubMed

Ohta, M; Hamako, J; Yamamoto, S; Hatta, H; Kim, M; Yamamoto, T; Oka, S; Mizuochi, T; Matsuura, F

1991-10-01

Asparagine-linked oligosaccharides present on hen egg-yolk immunoglobulin, termed IgY, were liberated from the protein by hydrazinolysis. After N-acetylation, the oligosaccharides were labelled with a UV-absorbing compound, p-aminobenzoic acid ethyl ester (ABEE). The ABEE-derivatized oligosaccharides were fractionated by anion exchange, normal phase and reversed phase HPLC, and their structures were determined by a combination of sugar composition analysis, methylation analysis, negative ion FAB-MS, 500 MHz 1H-NMR and sequential exoglycosidase digestions. IgY contained monoglucosylated oligomannose type oligosaccharides with structures of Glc alpha 1-3Man7-9-GlcNAc-GlcNAc, oligomannose type oligosaccharides with the size range of Man5-9GlcNAc-GlcNAc, and biantennary complex type oligosaccharides with core region structure of Man alpha 1-6(+/- GlcNAc beta 1-4)(Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(+/- Fuc alpha 1-6)GlcNAc. The glucosylated oligosaccharides, Glc1Man8GlcNAc2 and Glc1Man7GlcNAc2, have not previously been reported in mature glycoproteins from any source.

Effect of pretreatment on simultaneous saccharification and fermentation of hardwood into acetone/butanol

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shah, M.M.; Song, S.K.; Lee, Y.Y.

1991-12-31

The effectiveness of pretreatments on hardwood substrate was investigated in connection with its subsequent conversion by simultaneous saccharification and fermentation (SSF), using Clostridium acetobutylicum. The main objectives of the pretreatment were to achieve efficient separation of lignin from carbohydrates, and to obtain maximum sugar yield on enzymatic hydrolysis of pretreated wood. Two methods have given promising results: (1) supercritical CO{sub 2}-SO{sub 2} treatment, and (2) monoethanolamine (MEA) treatment. The MEA pretreatment removed above 90% of hardwood lignin while retaining 83% of carbohydrates. With CO{sub 2}-SO{sub 2} pretreatment, the degree of lignin separation was lower. Under the scheme of SSF, themore » pretreated hardwood was converted to acetone, butanol, and ethanol (ABE) via single stage processing by cellulose enzyme system and C. acetobutylicum cells. The product yield in the process was such that 15 g of ABE/100 g of dry aspen wood was produced. In the overall process of SSF, the enzymatic hydrolysis was found to be the rate-limiting step. The ability of C. acetobutylicum to metabolize various 6-carbon and 5-carbon sugars resulted in efficient utilization of all available sugars from hardwood.« less

Industrial production of acetone and butanol by fermentation-100 years later.

PubMed

Sauer, Michael

2016-07-01

Microbial production of acetone and butanol was one of the first large-scale industrial fermentation processes of global importance. During the first part of the 20th century, it was indeed the second largest fermentation process, superseded in importance only by the ethanol fermentation. After a rapid decline after the 1950s, acetone-butanol-ethanol (ABE) fermentation has recently gained renewed interest in the context of biorefinery approaches for the production of fuels and chemicals from renewable resources. The availability of new methods and knowledge opens many new doors for industrial microbiology, and a comprehensive view on this process is worthwhile due to the new interest. This thematic issue of FEMS Microbiology Letters, dedicated to the 100th anniversary of the first industrial exploitation of Chaim Weizmann's ABE fermentation process, covers the main aspects of old and new developments, thereby outlining a model development in biotechnology. All major aspects of industrial microbiology are exemplified by this single process. This includes new technologies, such as the latest developments in metabolic engineering, the exploitation of biodiversity and discoveries of new regulatory systems such as for microbial stress tolerance, as well as technological aspects, such as bio- and down-stream processing. © FEMS 2016.

EPPS: Efficient and Privacy-Preserving Personal Health Information Sharing in Mobile Healthcare Social Networks

PubMed Central

Jiang, Shunrong; Zhu, Xiaoyan; Wang, Liangmin

2015-01-01

Mobile healthcare social networks (MHSNs) have emerged as a promising next-generation healthcare system, which will significantly improve the quality of life. However, there are many security and privacy concerns before personal health information (PHI) is shared with other parities. To ensure patients’ full control over their PHI, we propose a fine-grained and scalable data access control scheme based on attribute-based encryption (ABE). Besides, policies themselves for PHI sharing may be sensitive and may reveal information about underlying PHI or about data owners or recipients. In our scheme, we let each attribute contain an attribute name and its value and adopt the Bloom filter to efficiently check attributes before decryption. Thus, the data privacy and policy privacy can be preserved in our proposed scheme. Moreover, considering the fact that the computational cost grows with the complexity of the access policy and the limitation of the resource and energy in a smart phone, we outsource ABE decryption to the cloud while preventing the cloud from learning anything about the content and access policy. The security and performance analysis is carried out to demonstrate that our proposed scheme can achieve fine-grained access policies for PHI sharing in MHSNs. PMID:26404300

EPPS: Efficient and Privacy-Preserving Personal Health Information Sharing in Mobile Healthcare Social Networks.

PubMed

Jiang, Shunrong; Zhu, Xiaoyan; Wang, Liangmin

2015-09-03

Mobile healthcare social networks (MHSNs) have emerged as a promising next-generation healthcare system, which will significantly improve the quality of life. However, there are many security and privacy concerns before personal health information (PHI) is shared with other parities. To ensure patients' full control over their PHI, we propose a fine-grained and scalable data access control scheme based on attribute-based encryption (ABE). Besides, policies themselves for PHI sharing may be sensitive and may reveal information about underlying PHI or about data owners or recipients. In our scheme, we let each attribute contain an attribute name and its value and adopt the Bloom filter to efficiently check attributes before decryption. Thus, the data privacy and policy privacy can be preserved in our proposed scheme. Moreover, considering the fact that the computational cost grows with the complexity of the access policy and the limitation of the resource and energy in a smart phone, we outsource ABE decryption to the cloud while preventing the cloud from learning anything about the content and access policy. The security and performance analysis is carried out to demonstrate that our proposed scheme can achieve fine-grained access policies for PHI sharing in MHSNs.

Enhanced mannan-derived fermentable sugars of palm kernel cake by mannanase-catalyzed hydrolysis for production of biobutanol.

PubMed

Shukor, Hafiza; Abdeshahian, Peyman; Al-Shorgani, Najeeb Kaid Nasser; Hamid, Aidil Abdul; Rahman, Norliza A; Kalil, Mohd Sahaid

2016-10-01

Catalytic depolymerization of mannan composition of palm kernel cake (PKC) by mannanase was optimized to enhance the release of mannan-derived monomeric sugars for further application in acetone-butanol-ethanol (ABE) fermentation. Efficiency of enzymatic hydrolysis of PKC was studied by evaluating effects of PKC concentration, mannanase loading, hydrolysis pH value, reaction temperature and hydrolysis time on production of fermentable sugars using one-way analysis of variance (ANOVA). The ANOVA results revealed that all factors studied had highly significant effects on total sugar liberated (P<0.01). The optimum conditions for PKC hydrolysis were 20% (w/v) PKC concentration, 5% (w/w) mannanase loading, hydrolysis pH 4.5, 45°C temperature and 72h hydrolysis time. Enzymatic experiments in optimum conditions revealed total fermentable sugars of 71.54±2.54g/L were produced including 67.47±2.51g/L mannose and 2.94±0.03g/L glucose. ABE fermentation of sugar hydrolysate by Clostridium saccharoperbutylacetonicum N1-4 resulted in 3.27±1.003g/L biobutanol. Copyright © 2016 Elsevier Ltd. All rights reserved.

Modeling of brittle-viscous flow using discrete particles

NASA Astrophysics Data System (ADS)

Thordén Haug, Øystein; Barabasch, Jessica; Virgo, Simon; Souche, Alban; Galland, Olivier; Mair, Karen; Abe, Steffen; Urai, Janos L.

2017-04-01

Many geological processes involve both viscous flow and brittle fractures, e.g. boudinage, folding and magmatic intrusions. Numerical modeling of such viscous-brittle materials poses challenges: one has to account for the discrete fracturing, the continuous viscous flow, the coupling between them, and potential pressure dependence of the flow. The Discrete Element Method (DEM) is a numerical technique, widely used for studying fracture of geomaterials. However, the implementation of viscous fluid flow in discrete element models is not trivial. In this study, we model quasi-viscous fluid flow behavior using Esys-Particle software (Abe et al., 2004). We build on the methodology of Abe and Urai (2012) where a combination of elastic repulsion and dashpot interactions between the discrete particles is implemented. Several benchmarks are presented to illustrate the material properties. Here, we present extensive, systematic material tests to characterize the rheology of quasi-viscous DEM particle packing. We present two tests: a simple shear test and a channel flow test, both in 2D and 3D. In the simple shear tests, simulations were performed in a box, where the upper wall is moved with a constant velocity in the x-direction, causing shear deformation of the particle assemblage. Here, the boundary conditions are periodic on the sides, with constant forces on the upper and lower walls. In the channel flow tests, a piston pushes a sample through a channel by Poisseuille flow. For both setups, we present the resulting stress-strain relationships over a range of material parameters, confining stress and strain rate. Results show power-law dependence between stress and strain rate, with a non-linear dependence on confining force. The material is strain softening under some conditions (which). Additionally, volumetric strain can be dilatant or compactant, depending on porosity, confining pressure and strain rate. Constitutive relations are implemented in a way that limits the range of viscosities. For identical pressure and strain rate, an order of magnitude range in viscosity can be investigated. The extensive material testing indicates that DEM particles interacting by a combination of elastic repulsion and dashpots can be used to model viscous flows. This allows us to exploit the fracturing capabilities of the discrete element methods and study systems that involve both viscous flow and brittle fracturing. However, the small viscosity range achievable using this approach does constraint the applicability for systems where larger viscosity ranges are required, such as folding of viscous layers of contrasting viscosities. References: Abe, S., Place, D., & Mora, P. (2004). A parallel implementation of the lattice solid model for the simulation of rock mechanics and earthquake dynamics. PAGEOPH, 161(11-12), 2265-2277. http://doi.org/10.1007/s00024-004-2562-x Abe, S., and J. L. Urai (2012), Discrete element modeling of boudinage: Insights on rock rheology, matrix flow, and evolution of geometry, JGR., 117, B01407, doi:10.1029/2011JB00855

Japanese Prime Minister Shinzo Abe’s Two Administrations: Successes and Failures of Domestic and Security Policies

DTIC Science & Technology

2016-12-01

administrations, Abe’s unrivaled political performance within the Liberal Democratic Party (LDP), the weakness of opposition parties, and an economic...political success and failure, domestic policy, security policy, pragmatism, political environment, normalization, Liberal Democratic Party (LDP) 15...level, political influence from former administrations, Abe’s unrivaled political performance within the Liberal Democratic Party (LDP), the weakness

M1 Abrams Tank Procedure Guides

DTIC Science & Technology

1982-07-01

Vemp =. ... . Mheek mustlam- sitlee no"Ageper- 2. Cal.5 . . swetgh: (PAge LI) 3. CaLS . ... Zen (pag 13) BOUzSzMT M CAL. SO I.. Teak peatima. .. lafel...Cleee ,-. 13.~~ .eds a." . .ege ..... abeW "d us (P P U) :. -z KINK CAL. 50 3. vempe " .. ... .led (pg ) 2. o . .. .ad tio"ge mgety .. 1 4. IC omee .C.1

New Approaches to Attenuated Hepatitis A Vaccine Development: Cloning and Sequencing of Cell-Culture Adapted Viral cDNA

DTIC Science & Technology

1989-10-01

FRhK-4 cells by BS-C-l-passaged hepatitis A virus. Appl.Environ.Microbiol. 53, 2967- 2971. NOMOTO, A., KOHARA , M., KUGE, S., KAWAMURA, N., ARITA, M...MA, Rueckert RR, eds. Positive Strand RNA Viruses. New York City:Alan R. Liss, pp 437-452. OMATA, T., KOHARA , M., KUGE, S., KOMATSU, T., ABE, S

Orbit Determination Using Vinti’s Solution

DTIC Science & Technology

2016-09-15

Surveillance Network STK Systems Tool Kit TBP Two Body Problem TLE Two-line Element Set xv Acronym Definition UKF Unscented Kalman Filter WPAFB Wright...simplicity, stability, and speed. On the other hand, Kalman filters would be best suited for sequential estimation of stochastic or random components of a...be likened to how an Unscented Kalman Filter samples a system’s nonlinearities directly, avoiding linearizing the dynamics in the partials matrices

Issues and Strategies for Improving Constructibility.

DTIC Science & Technology

1988-09-01

materials. First, the roof design called for the use of an asphalt coated roof felt layer below an EPDM membrane. The asphalt coated felt is not needed when a...being prepared by people trained in subjects foreign to construction. As designers, we were in fact contractually and professionally isolated from...specially constructed for sound isolation . The architect* correctly specified special sound seals around the doors between the rooms in this area, but

The Islamic State We Knew: Insights Before the Resurgence and Their Implications

DTIC Science & Technology

2015-01-01

smuggling process, such as the physical distribution process, and it can also broaden the fight against both oil and antiquities smuggling by...extortion, and create weapon shops . Observing a pattern of intimidation and assassinations against established government authorities can provide a...be targeted. This would degrade the Islamic State’s ability to sell oil and refined products. To broaden the fight against both oil and antiquities

A carbon nanotube filled polydimethylsiloxane hybrid membrane for enhanced butanol recovery

PubMed Central

Xue, Chuang; Du, Guang-Qing; Chen, Li-Jie; Ren, Jian-Gang; Sun, Jian-Xin; Bai, Feng-Wu; Yang, Shang-Tian

2014-01-01

The carbon nanotubes (CNTs) filled polydimethylsiloxane (PDMS) hybrid membrane was fabricated to evaluate its potential for butanol recovery from acetone-butanol-ethanol (ABE) fermentation broth. Compared with the homogeneous PDMS membrane, the CNTs filled into the PDMS membrane were beneficial for the improvement of butanol recovery in butanol flux and separation factor. The CNTs acting as sorption-active sites with super hydrophobicity could give an alternative route for mass transport through the inner tubes or along the smooth surface. The maximum total flux and butanol separation factor reached up to 244.3 g/m2·h and 32.9, respectively, when the PDMS membrane filled with 10 wt% CNTs was used to separate butanol from the butanol/water solution at 80°C. In addition, the butanol flux and separation factor increased dramatically as temperature increased from 30°C to 80°C in feed solution since the higher temperature produced more free volumes in polymer chains to facilitate butanol permeation. A similar increase was also observed when butanol titer in solution increased from 10 g/L to 25 g/L. Overall, the CNTs/PDMS hybrid membrane with higher butanol flux and selectivity should have good potential for pervaporation separation of butanol from ABE fermentation broth. PMID:25081019

Improving the performance of solventogenic clostridia by reinforcing the biotin synthetic pathway.

PubMed

Yang, Yunpeng; Lang, Nannan; Yang, Gaohua; Yang, Sheng; Jiang, Weihong; Gu, Yang

2016-05-01

An efficient production process is important for industrial microorganisms. The cellular efficiency of solventogenic clostridia, a group of anaerobes capable of producing a wealth of bulk chemicals and biofuels, must be improved for competitive commercialization. Here, using Clostridium acetobutylicum, a species of solventogenic clostridia, we revealed that the insufficient biosynthesis of biotin, a pivotal coenzyme for many important biological processes, is a major limiting bottleneck in this anaerobe's performance. To address this problem, we strengthened the biotin synthesis of C. acetobutylicum by overexpressing four relevant genes involved in biotin transport and biosynthesis. This strategy led to faster growth and improved the titer and productivity of acetone, butanol and ethanol (ABE solvents) of C. acetobutylicum in both biotin-containing and biotin-free media. Expressionally modulating these four genes by modifying the ribosome binding site further promoted cellular performance, achieving ABE solvent titer and productivity as high as 21.9g/L and 0.30g/L/h, respectively, in biotin-free medium; these values exceeded those of the wild-type strain by over 30%. More importantly, biotin synthesis reinforcement also conferred improved ability of C. acetobutylicum to use hexose and pentose sugars, further demonstrating the potential of this metabolic-engineering strategy in solventogenic clostridia. Copyright © 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

Detoxification of Organosolv-Pretreated Pine Prehydrolysates with Anion Resin and Cysteine for Butanol Fermentation.

PubMed

Li, Jing; Shi, Suan; Tu, Maobing; Via, Brain; Sun, Fubao Fuelbio; Adhikari, Sushil

2018-05-02

Bioconversion of lignocellulose to biofuels suffers from the degradation compounds formed during pretreatment and acid hydrolysis. In order to achieve an efficient biomass to biofuel conversion, detoxification is often required before enzymatic hydrolysis and microbial fermentation. Prehydrolysates from ethanol organosolv-pretreated pine wood were used as substrates in butanol fermentation in this study. Six detoxification approaches were studied and compared, including overliming, anion exchange resin, nonionic resin, laccase, activated carbon, and cysteine. It was observed that detoxification by anion exchange resin was the most effective method. The final butanol yield after anion exchange resin treatment was comparable to the control group, but the fermentation was delayed for 72 h. The addition of Ca(OH) 2 was found to alleviate this delay and improve the fermentation efficiency. The combination of Ca(OH) 2 and anion exchange resin resulted in completion of fermentation within 72 h and acetone-butanol-ethanol (ABE) production of 11.11 g/L, corresponding to a yield of 0.21 g/g sugar. The cysteine detoxification also resulted in good detoxification performance, but promoted fermentation towards acid production (8.90 g/L). The effect of salt on ABE fermentation was assessed and the possible role of Ca(OH) 2 was to remove the salts in the prehydrolysates by precipitation.

New Insight into Sugarcane Industry Waste Utilization (Press Mud) for Cleaner Biobutanol Production by Using C. acetobutylicum NRRL B-527.

PubMed

Nimbalkar, Pranhita R; Khedkar, Manisha A; Gaikwad, Shashank G; Chavan, Prakash V; Bankar, Sandip B

2017-11-01

In the present study, press mud, a sugar industry waste, was explored for biobutanol production to strengthen agricultural economy. The fermentative production of biobutanol was investigated via series of steps, viz. characterization, drying, acid hydrolysis, detoxification, and fermentation. Press mud contains an adequate amount of cellulose (22.3%) and hemicellulose (21.67%) on dry basis, and hence, it can be utilized for further acetone-butanol-ethanol (ABE) production. Drying experiments were conducted in the temperature range of 60-120 °C to circumvent microbial spoilage and enhance storability of press mud. Furthermore, acidic pretreatment variables, viz. sulfuric acid concentration, solid to liquid ratio, and time, were optimized using response surface methodology. The corresponding values were found to be 1.5% (v/v), 1:5 g/mL, and 15 min, respectively. In addition, detoxification studies were also conducted using activated charcoal, which removed almost 93-97% phenolics and around 98% furans, which are toxic to microorganisms during fermentation. Finally, the batch fermentation of detoxified press mud slurry (the sample dried at 100 °C and pretreated) using Clostridium acetobutylicum NRRL B-527 resulted in a higher butanol production of 4.43 g/L with a total ABE of 6.69 g/L.

High-resolution H -band Spectroscopy of Be Stars with SDSS-III/APOGEE. II. Line Profile and Radial Velocity Variability

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chojnowski, S. Drew; Holtzman, Jon A.; Wisniewski, John P.

2017-04-01

We report on the H -band spectral variability of classical Be stars observed over the course of the Apache Point Galactic Evolution Experiment (APOGEE), one of four subsurveys comprising SDSS-III. As described in the first paper of this series, the APOGEE B-type emission-line (ABE) star sample was culled from the large number of blue stars observed as telluric standards during APOGEE observations. In this paper, we explore the multi-epoch ABE sample, consisting of 1100 spectra for 213 stars. These “snapshots” of the circumstellar disk activity have revealed a wealth of temporal variability including, but not limited to, gradual disappearance ofmore » the line emission and vice versa over both short and long timescales. Other forms of variability include variation in emission strength, emission peak intensity ratios, and emission peak separations. We also analyze radial velocities (RVs) of the emission lines for a subsample of 162 stars with sufficiently strong features, and we discuss on a case-by-case basis whether the RV variability exhibited by some stars is caused by binary motion versus dynamical processes in the circumstellar disks. Ten systems are identified as convincing candidates for binary Be stars with as of yet undetected companions.« less

Development of a high-throughput assay for rapid screening of butanologenic strains.

PubMed

Agu, Chidozie Victor; Lai, Stella M; Ujor, Victor; Biswas, Pradip K; Jones, Andy; Gopalan, Venkat; Ezeji, Thaddeus Chukwuemeka

2018-02-21

We report a Thermotoga hypogea (Th) alcohol dehydrogenase (ADH)-dependent spectrophotometric assay for quantifying the amount of butanol in growth media, an advance that will facilitate rapid high-throughput screening of hypo- and hyper-butanol-producing strains of solventogenic Clostridium species. While a colorimetric nitroblue tetrazolium chloride-based assay for quantitating butanol in acetone-butanol-ethanol (ABE) fermentation broth has been described previously, we determined that Saccharomyces cerevisiae (Sc) ADH used in this earlier study exhibits approximately 13-fold lower catalytic efficiency towards butanol than ethanol. Any Sc ADH-dependent assay for primary quantitation of butanol in an ethanol-butanol mixture is therefore subject to "ethanol interference". To circumvent this limitation and better facilitate identification of hyper-butanol-producing Clostridia, we searched the literature for native ADHs that preferentially utilize butanol over ethanol and identified Th ADH as a candidate. Indeed, recombinant Th ADH exhibited a 6-fold higher catalytic efficiency with butanol than ethanol, as measured using the reduction of NADP + to NADPH that accompanies alcohol oxidation. Moreover, the assay sensitivity was not affected by the presence of acetone, acetic acid or butyric acid (typical ABE fermentation products). We broadened the utility of our assay by adapting it to a high-throughput microtiter plate-based format, and piloted it successfully in an ongoing metabolic engineering initiative.

Secure, Policy-Based, Multi-Recipient Data Sharing

DTIC Science & Technology

2009-01-01

effects of a new drug to all patients at participating hospitals, who have a certain medical condition and have indicated a willingness to partici...pate in clinical trials at their discretion. The drug company considers the potential side effects of the new drug private data and thus would like to...health care example discussed above. Attribute-Based Encryption (ABE) [63, 37, 10, 23, 56, 24, 55, 36, 43, 49] is a new public-key encryption paradigm

Honoring Navy SEALs Petty Officer 2nd Class Matthew McCabe, Petty Officer 2nd Class Jonathan Keefe, and Petty Officer 1st Class Julio Huertas for their heroic actions in the capture of Ahmed Hashim Abed, the mastermind behind of one of the most notorious crimes against Americans in Iraq.

THOMAS, 111th Congress

Rep. Poe, Ted [R-TX-2

2009-12-16

House - 01/21/2010 Referred to the Subcommittee on Terrorism, Unconventional Threats and Capabilities. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Distinct Circulating Recombinant HIV-1 Strains Among Injecting Drug Users and Sex Workers in Afghanistan

DTIC Science & Technology

2010-05-01

Kabul, and Mazar-i-Sharif, Afghanistan. This cross-sectional study conducted between September 2006 and January 2008 surveyed SWs from Kabul...nonmonetary gift of hy- giene and grooming supplies (e.g., shampoo , toothpaste), whereas IDU participants received a small nonmonetary gift of hygiene...Nations Office on Drugs and Crime (UNODC): Afghanistan Drug Use Survey 2005. Kabul, Afghanistan. 2005. 10. Sanders-Buell E, Saad MD, Abed AS, et al

Controlling the type I error rate in two-stage sequential adaptive designs when testing for average bioequivalence.

PubMed

Maurer, Willi; Jones, Byron; Chen, Ying

2018-05-10

In a 2×2 crossover trial for establishing average bioequivalence (ABE) of a generic agent and a currently marketed drug, the recommended approach to hypothesis testing is the two one-sided test (TOST) procedure, which depends, among other things, on the estimated within-subject variability. The power of this procedure, and therefore the sample size required to achieve a minimum power, depends on having a good estimate of this variability. When there is uncertainty, it is advisable to plan the design in two stages, with an interim sample size reestimation after the first stage, using an interim estimate of the within-subject variability. One method and 3 variations of doing this were proposed by Potvin et al. Using simulation, the operating characteristics, including the empirical type I error rate, of the 4 variations (called Methods A, B, C, and D) were assessed by Potvin et al and Methods B and C were recommended. However, none of these 4 variations formally controls the type I error rate of falsely claiming ABE, even though the amount of inflation produced by Method C was considered acceptable. A major disadvantage of assessing type I error rate inflation using simulation is that unless all possible scenarios for the intended design and analysis are investigated, it is impossible to be sure that the type I error rate is controlled. Here, we propose an alternative, principled method of sample size reestimation that is guaranteed to control the type I error rate at any given significance level. This method uses a new version of the inverse-normal combination of p-values test, in conjunction with standard group sequential techniques, that is more robust to large deviations in initial assumptions regarding the variability of the pharmacokinetic endpoints. The sample size reestimation step is based on significance levels and power requirements that are conditional on the first-stage results. This necessitates a discussion and exploitation of the peculiar properties of the power curve of the TOST testing procedure. We illustrate our approach with an example based on a real ABE study and compare the operating characteristics of our proposed method with those of Method B of Povin et al. Copyright © 2018 John Wiley & Sons, Ltd.

Normal Fault Type Earthquakes Off Fukushima Region - Comparison of the 1938 Events and Recent Earthquakes -

NASA Astrophysics Data System (ADS)

Murotani, S.; Satake, K.

2017-12-01

Off Fukushima region, Mjma 7.4 (event A) and 6.9 (event B) events occurred on November 6, 1938, following the thrust fault type earthquakes of Mjma 7.5 and 7.3 on the previous day. These earthquakes were estimated as normal fault earthquakes by Abe (1977, Tectonophysics). An Mjma 7.0 earthquake occurred on July 12, 2014 near event B and an Mjma 7.4 earthquake occurred on November 22, 2016 near event A. These recent events are the only M 7 class earthquakes occurred off Fukushima since 1938. Except for the two 1938 events, normal fault earthquakes have not occurred until many aftershocks of the 2011 Tohoku earthquake. We compared the observed tsunami and seismic waveforms of the 1938, 2014, and 2016 earthquakes to examine the normal fault earthquakes occurred off Fukushima region. It is difficult to compare the tsunami waveforms of the 1938, 2014 and 2016 events because there were only a few observations at the same station. The teleseismic body wave inversion of the 2016 earthquake yielded with the focal mechanism of strike 42°, dip 35°, and rake -94°. Other source parameters were as follows: source area 70 km x 40 km, average slip 0.2 m, maximum slip 1.2 m, seismic moment 2.2 x 1019 Nm, and Mw 6.8. A large slip area is located near the hypocenter, and it is compatible with the tsunami source area estimated from tsunami travel times. The 2016 tsunami source area is smaller than that of the 1938 event, consistent with the difference in Mw: 7.7 for event A estimated by Abe (1977) and 6.8 for the 2016 event. Although the 2014 epicenter is very close to that of event B, the teleseismic waveforms of the 2014 event are similar to those of event A and the 2016 event. While Abe (1977) assumed that the mechanism of event B was the same as event A, the initial motions at some stations are opposite, indicating that the focal mechanisms of events A and B are different and more detailed examination is needed. The normal fault type earthquake seems to occur following the occurrence of M7 9 class thrust type earthquake at the plate boundary off Fukushima region.

Butanol production under microaerobic conditions with a symbiotic system of Clostridium acetobutylicum and Bacillus cereus.

PubMed

Wu, Pengfei; Wang, Genyu; Wang, Gehua; Børresen, Børre Tore; Liu, Hongjuan; Zhang, Jianan

2016-01-14

One major problem of ABE (acetone, butanol and ethanol) fermentation is high oxygen sensitivity of Clostridium acetobutylicum. Currently, no single strain has been isolated or genetically engineered to produce butanol effectively under aerobic conditions. In our previous work, a symbiotic system TSH06 has been developed successfully by our group, and two strains, C. acetobutylicum TSH1 and Bacillus cereus TSH2, were isolated from TSH06. Compared with single culture, TSH06 showed promotion on cell growth and solvent accumulation under microaerobic conditions. To simulate TSH06, a new symbiotic system was successfully re-constructed by adding living cells of B. cereus TSH2 into C. acetobutylicum TSH1 cultures. During the fermentation process, the function of B. cereus TSH2 was found to deplete oxygen and provide anaerobic environment for C. acetobutylicum TSH1. Furthermore, inoculation ratio of C. acetobutylicum TSH1 and B. cereus TSH2 affected butanol production. In a batch fermentation with optimized inoculation ratio of 5 % C. acetobutylicum TSH1 and 0.5 % B. cereus TSH2, 11.0 g/L butanol and 18.1 g/L ABE were produced under microaerobic static condition. In contrast to the single culture of C. acetobutylicum TSH1, the symbiotic system became more aerotolerant and was able to produce 11.2 g/L butanol in a 5 L bioreactor even with continuous 0.15 L/min air sparging. In addition, qPCR assay demonstrated that the abundance of B. cereus TSH2 increased quickly at first and then decreased sharply to lower than 1 %, whereas C. acetobutylicum TSH1 accounted for more than 99 % of the whole population in solventogenic phase. The characterization of a novel symbiotic system on butanol fermentation was studied. The new symbiotic system re-constructed by co-culture of C. acetobutylicum TSH1 and B. cereus TSH2 showed excellent performance on butanol production under microaerobic conditions. B. cereus TSH2 was a good partner for C. acetobutylicum TSH1 by providing an anaerobic environment. During fermentation process, the high ratio of Clostridium and low ratio of Bacillus composition indicated that this symbiotic system was an effective and easily controlled cultivation model for ABE fermentation under microaerobic conditions.

Air & Space Power Journal. Volume 29, Number 5, September-October 2015

DTIC Science & Technology

2015-10-01

Views Changing the Tooth-to-Tail Ratio Using Robotics and Automation to Beat Sequestration ❙ 75 Capt Rachael L. Nussbaum, USAF Twenty-First-Century Air...technophobes who see this aircraft as some sort of advanced war-fighting robot . As with any other aircraft, the heart of the system remains the aircrew...technology -quarterly/21567205-abe-karem-created- robotic -plane-transformed-way-modern-warfare. 36. Blair and Helms, “Swarm, the Cloud,” 29–33. 37

Investigation of New Subsystem Concepts to Improve the Operational and Hydrodynamic Capabilities of Advanced Amphibian Vehicles

DTIC Science & Technology

1980-06-01

Appendix C), only the following three offered even a slight hope that they might be useful to amphibian developments: the Amphibious Air Track, page A...be associated with future amphibians. Within the past year, the University of Michigan College of Engineering has established the Office for the...Land Interface: Claude A. Blackmon, Beryle G. Stinson Jack K. Stoll AD 881 357 Engineering Design Handbook; Wheeled Amphbians: No author listed AD

Action video game play and transfer of navigation and spatial cognition skills in adolescents who are blind.

PubMed

Connors, Erin C; Chrastil, Elizabeth R; Sánchez, Jaime; Merabet, Lotfi B

2014-01-01

For individuals who are blind, navigating independently in an unfamiliar environment represents a considerable challenge. Inspired by the rising popularity of video games, we have developed a novel approach to train navigation and spatial cognition skills in adolescents who are blind. Audio-based Environment Simulator (AbES) is a software application that allows for the virtual exploration of an existing building set in an action video game metaphor. Using this ludic-based approach to learning, we investigated the ability and efficacy of adolescents with early onset blindness to acquire spatial information gained from the exploration of a target virtual indoor environment. Following game play, participants were assessed on their ability to transfer and mentally manipulate acquired spatial information on a set of navigation tasks carried out in the real environment. Success in transfer of navigation skill performance was markedly high suggesting that interacting with AbES leads to the generation of an accurate spatial mental representation. Furthermore, there was a positive correlation between success in game play and navigation task performance. The role of virtual environments and gaming in the development of mental spatial representations is also discussed. We conclude that this game based learning approach can facilitate the transfer of spatial knowledge and further, can be used by individuals who are blind for the purposes of navigation in real-world environments.

Action video game play and transfer of navigation and spatial cognition skills in adolescents who are blind

PubMed Central

Connors, Erin C.; Chrastil, Elizabeth R.; Sánchez, Jaime; Merabet, Lotfi B.

2014-01-01

For individuals who are blind, navigating independently in an unfamiliar environment represents a considerable challenge. Inspired by the rising popularity of video games, we have developed a novel approach to train navigation and spatial cognition skills in adolescents who are blind. Audio-based Environment Simulator (AbES) is a software application that allows for the virtual exploration of an existing building set in an action video game metaphor. Using this ludic-based approach to learning, we investigated the ability and efficacy of adolescents with early onset blindness to acquire spatial information gained from the exploration of a target virtual indoor environment. Following game play, participants were assessed on their ability to transfer and mentally manipulate acquired spatial information on a set of navigation tasks carried out in the real environment. Success in transfer of navigation skill performance was markedly high suggesting that interacting with AbES leads to the generation of an accurate spatial mental representation. Furthermore, there was a positive correlation between success in game play and navigation task performance. The role of virtual environments and gaming in the development of mental spatial representations is also discussed. We conclude that this game based learning approach can facilitate the transfer of spatial knowledge and further, can be used by individuals who are blind for the purposes of navigation in real-world environments. PMID:24653690

Nesterenkonia sp. strain F, a halophilic bacterium producing acetone, butanol, and ethanol under aerobic conditions.

PubMed

Amiri, Hamid; Azarbaijani, Reza; Parsa Yeganeh, Laleh; Shahzadeh Fazeli, Abolhassan; Tabatabaei, Meisam; Salekdeh, Ghasem Hosseini; Karimi, Keikhosro

2016-01-04

The moderately halophilic bacterium Nesterenkonia sp. strain F, which was isolated from Aran-Bidgol Lake (Iran), has the ability to produce acetone, butanol, and ethanol (ABE) as well as acetic and butyric acids under aerobic and anaerobic conditions. This result is the first report of ABE production with a wild microorganism from a family other than Clostridia and also the first halophilic species shown to produce butanol under aerobic cultivation. The cultivation of Nesterenkonia sp. strain F under anaerobic conditions with 50 g/l of glucose for 72 h resulted in the production of 105 mg/l of butanol, 122 mg/l of acetone, 0.2 g/l of acetic acid, and 2.5 g/l of butyric acid. Furthermore, the strain was cultivated on media with different glucose concentrations (20, 50, and 80 g/l) under aerobic and anaerobic conditions. Through fermentation with a 50 g/l initial glucose concentration under aerobic conditions, 66 mg/l of butanol, 125 mg/l of acetone, 291 mg/l of ethanol, 5.9 g/l of acetic acid, and 1.2 g/l of butyric acid were produced. The enzymes pertaining to the fermentation pathway in the strain were compared with the enzymes of Clostridium spp., and the metabolic pathway of fermentation used by Nesterenkonia sp. strain F was investigated.

Efficacy of testicular sperm chromatin condensation assay using aniline blue-eosin staining in the IVF-ET cycle.

PubMed

Park, Yong-Seog; Kim, Myo Kyung; Lee, Sun-Hee; Cho, Jae Won; Song, In Ok; Seo, Ju Tae

2011-09-01

This study was performed to evaluate testicular sperm chromatin condensation using aniline blue-eosin (AB-E) staining and its effects on IVF-ET. Chromatin condensation was analyzed using AB-E staining in 27 cases of testicular sperm extraction. There were 19 cases of obstructive azoospermia (OA) and 8 cases of non-obstructive azoospermia (NOA) in IVF-ET. Mature sperm heads were stained red-pink whereas immature sperm heads were stained dark blue. The percentage of sperm chromatin condensation was calculated from the ratio of the number of red-pink sperm to the total number of sperm analyzed. The overall percentages of chromatin condensation in OA and NOA were 31.1±11.2% and 26.3±14.4%, respectively. The fertilization rate was significant higher in OA than NOA (p<0.05); however, the rates of good embryos and clinical pregnancy did not show statistical differences. In OA and NOA, statistical differences were not observed in the rate of chromatin condensation, fertilization, good embryos, and clinical pregnancy between the pregnant group and non-pregnant group. Chromatin condensation is less stable than OA and showed a low fertilization rate in NOA. While there were no significant differences in chromatin condensation results between NOA and OA, we propose that a pattern of decreased chromatin condensation in NOA is one of the factors of low fertilization results requiring further study.

Enhancing Butanol Production under the Stress Environments of Co-Culturing Clostridium acetobutylicum/Saccharomyces cerevisiae Integrated with Exogenous Butyrate Addition

PubMed Central

Luo, Hongzhen; Ge, Laibing; Zhang, Jingshu; Zhao, Yanli; Ding, Jian; Li, Zhigang; He, Zhenni; Chen, Rui; Shi, Zhongping

2015-01-01

In this study, an efficient acetone-butanol-ethanol (ABE) fermentation strategy integrating Clostridium acetobutylicum/Saccharomyces cerevisiae co-culturing system with exogenous butyrate addition, was proposed and experimentally conducted. In solventogenic phase, by adding 0.2 g-DCW/L-broth viable S. cerevisiae cells and 4.0 g/L-broth concentrated butyrate solution into C. acetobutylicum culture broth, final butanol concentration and butanol/acetone ratio in a 7 L anaerobic fermentor reached the highest levels of 15.74 g/L and 2.83 respectively, with the increments of 35% and 43% as compared with those of control. Theoretical and experimental analysis revealed that, the proposed strategy could, 1) extensively induce secretion of amino acids particularly lysine, which are favorable for both C. acetobutylicum survival and butanol synthesis under high butanol concentration environment; 2) enhance the utilization ability of C. acetobutylicum on glucose and over-produce intracellular NADH for butanol synthesis in C. acetobutylicum metabolism simultaneously; 3) direct most of extra consumed glucose into butanol synthesis route. The synergetic actions of effective amino acids assimilation, high rates of substrate consumption and NADH regeneration yielded highest butanol concentration and butanol ratio in C. acetobutylicum under this stress environment. The proposed method supplies an alternative way to improve ABE fermentation performance by traditional fermentation technology. PMID:26489085

Continuous bio-catalytic conversion of sugar mixture to acetone-butanol-ethanol by immobilized Clostridium acetobutylicum DSM 792.

PubMed

Survase, Shrikant A; van Heiningen, Adriaan; Granström, Tom

2012-03-01

Continuous production of acetone, n-butanol, and ethanol (ABE) was carried out using immobilized cells of Clostridium acetobutylicum DSM 792 using glucose and sugar mixture as a substrate. Among various lignocellulosic materials screened as a support matrix, coconut fibers and wood pulp fibers were found to be promising in batch experiments. With a motive of promoting wood-based bio-refinery concept, wood pulp was used as a cell holding material. Glucose and sugar mixture (glucose, mannose, galactose, arabinose, and xylose) comparable to lignocellulose hydrolysate was used as a substrate for continuous production of ABE. We report the best solvent productivity among wild-type strains using column reactor. The maximum total solvent concentration of 14.32 g L(-1) was obtained at a dilution rate of 0.22 h(-1) with glucose as a substrate compared to 12.64 g L(-1) at 0.5 h(-1) dilution rate with sugar mixture. The maximum solvent productivity (13.66 g L(-1) h(-1)) was obtained at a dilution rate of 1.9 h(-1) with glucose as a substrate whereas solvent productivity (12.14 g L(-1) h(-1)) was obtained at a dilution rate of 1.5 h(-1) with sugar mixture. The immobilized column reactor with wood pulp can become an efficient technology to be integrated with existing pulp mills to convert them into wood-based bio-refineries.

High-Average Power Broadband 18-Beam Klystron Circuit and Collector Designs

DTIC Science & Technology

2008-04-01

high -average power S - band multiple-beam klystron are presented. The klystron will be powered by the recently completed 41.6 A, 42...al., “ High - power Four-cavity S - band multiple-beam klystron design,” IEEE Trans. Plasma Science, vol. 33, pp. 1119-1135, April 2005. [3] D.K Abe, et...APR 2008 2. REPORT TYPE 3. DATES COVERED 00-00-2008 to 00-00-2008 4. TITLE AND SUBTITLE High -average Power Broadband 18-beam

Low-Pressure Capability of NASA Glenn's 10- by 10-Foot Supersonic Wind Tunnel Expanded

NASA Technical Reports Server (NTRS)

Roeder, James W.

2004-01-01

Extremely low dynamic pressure Q conditions are desired for space-related research including the testing of parachute designs and other decelerator concepts for future vehicles landing on Mars. Therefore, the low-pressure operating capability of the Abe Silverstein 10- by 10-foot Supersonic Wind Tunnel (10 10 SWT) at NASA Glenn Research Center was recently increased. Successful checkout tests performed in the fall of 2002 showed significantly reduced minimum operating pressures in the wind tunnel.

Description of the hemipenial morphology of Tupinambis quadrilineatus Manzani and Abe, 1997 (Squamata, Teiidae) and new records from Piauí, Brazil

PubMed Central

da Silva, Marcélia Basto; de Lima-Filho, Geraldo Rodrigues; Cronemberger, Áurea Aguiar; Carvalho, Leonardo Sousa; Manzani, Paulo Roberto; Vieira, Jânia Brito

2013-01-01

Abstract Few data are available on the morphology of the hemipenis of teiid lizards, especially those of the recently-defined genus Tupinambis, a widely-distributed group of large-bodied lizards. This study provides an illustrated description of the hemipenis of Tupinambis quadrilineatus, which is similar to that of other representatives of the Tupinambinae subfamily. New records of the species from the state of Piauí, in northeastern Brazil, are also presented. PMID:24363597

Recognizing the exemplarily service, devotion to country, and selfless sacrifice of Special Warfare Operators 2nd Class Matthew McCabe and Jonathan Keefe and Special Warfare Operator 1st Class Julio Huertas in capturing Ahmed Hashim Abed, one of the most-wanted terrorists in Iraq, and pledging to continue to support members of the United States Armed Forces serving in harm's way.

THOMAS, 111th Congress

Rep. Hunter, Duncan D. [R-CA-52

2009-12-16

House - 01/21/2010 Referred to the Subcommittee on Terrorism, Unconventional Threats and Capabilities. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

The Flux OSKit: A Substrate for Kernel and Language Research

DTIC Science & Technology

1997-10-01

unclassified Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18 tions. Our own microkernel -based OS, Fluke [17], puts almost all of the OSKit to use...kernels distance the language from the hardware; even microkernels and other extensible kernels enforce some default policy which often conflicts with a...be particu- larly useful in these research projects. 6.1.1 The Fluke OS In 1996 we developed an entirely new microkernel - based system called Fluke

Germanium-Source Tunnel Field Effect Transistors for Ultra-Low Power Digital Logic

DTIC Science & Technology

2012-05-10

carrier injection via band-to-band tunneling (BTBT) and the absence of thermal (kT) dependence allows for the subthreshold swing to be steeper than... tunneling probability was derived by Kane using time- dependent perturbation theory and Fermi’s Golden Rule [8-9]. This section will instead employ a...be based on tunneling across a reverse- biased p-n junction as shown in Fig. 2.2. In order to obtain a closed form solution of the BTBT

Vasopressin Receptor Signaling and Cycling of Water Channels in Renal Epithelia.

DTIC Science & Technology

1994-08-31

bladder (Bentley, 1958; DiBona et al., 1969; Hays, 1983; Mia et al., 1983, 1987, 1991a and others). ADH stimulates water reabsorption in this tissue...out of a continuous phase of microridges (Davis et al., 1974; DiBona , 1978, 1981; Dratwa et al., 1979; Mills and Malick, 1978; Mia et al., 1983, 1988a...be directed by dynamic actions of the microtubules and microfilaments (Hays et al., 1982; Taylor et al., 1973; Hardy and DiBona , 1982; Pearl and Taylor

Improving Fructose Utilization and Butanol Production by Clostridium acetobutylicum via Extracellular Redox Potential Regulation and Intracellular Metabolite Analysis.

PubMed

Chen, Li-Jie; Wu, You-Duo; Xue, Chuang; Bai, Feng-Wu

2017-10-01

Jerusalem artichoke (JA) can grow well in marginal lands with high biomass yield, and thus is a potential energy crop for biorefinery. The major biomass of JA is from tubers, which contain inulin that can be easily hydrolyzed into a mixture of fructose and glucose, but fructose utilization for producing butanol as an advanced biofuel is poor compared to glucose-based ABE fermentation by Clostridium acetobutylicum. In this article, the impact of extracellular redox potential (ORP) on the process is studied using a mixture of fructose and glucose to simulate the hydrolysate of JA tubers. When the extracellular ORP is controlled above -460 mV, 13.2 g L -1 butanol is produced from 51.0 g L -1 total sugars (40.1 g L -1 fructose and 10.9 g L -1 glucose), leading to dramatically increased butanol yield and butanol/ABE ratio of 0.26 g g -1 and 0.67, respectively. Intracellular metabolite and q-PCR analysis further indicate that intracellular ATP and NADH availabilities are significantly improved together with the fructose-specific PTS expression at the lag phase, which consequently facilitate fructose transport, metabolic shift toward solventogenesis and carbon flux redistribution for butanol biosynthesis. Therefore, the extracellular ORP control can be an effective strategy to improve butanol production from fructose-based feedstock. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A New Contribution for WYP 2005: The Golden Ratio, Bohr Radius, Planck's Constant, Fine-Structure Constant and g-Factors

NASA Astrophysics Data System (ADS)

Heyrovska, R.; Narayan, S.

2005-10-01

Recently, the ground state Bohr radius (aB) of hydrogen was shown to be divided into two Golden sections, aB,p = aB/ø2 and aB,e = aB/ø at the point of electrical neutrality, where ø = 1.618 is the Golden ratio (R. Heyrovska, Molecular Physics 103: 877-882, and the literature cited therein). The origin of the difference of two energy terms in the Rydberg equation was thus shown to be in the ground state energy itself, as shown below: EH = (1/2)e2/(κaB) = (1/2)(e2/κ) [(1/aB,p - (1/aB,e)] (1). This work brings some new results that 1) a unit charge in vacuum has a magnetic moment, 2) (e2/2κ) in eq. (1) is an electromagnetic condenser constant, 3) the de Broglie wavelengths of the proton and electron correspond to the Golden arcs of a circle with the Bohr radius, 4) the fine structure constant (α) is the ratio of the Planck's constants without and with the interaction of light with matter, 5) the g-factors of the electron and proton, ge/2 and gp/2 divide the Bohr radius at the magnetic center and 6) the ``mysterious'' value (137.036) of α -1 = (360/ø2) - (2/ø3), where (2/ø3) arises from the difference, (gp - ge).

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium.

PubMed

Wen, Zhiqiang; Minton, Nigel P; Zhang, Ying; Li, Qi; Liu, Jinle; Jiang, Yu; Yang, Sheng

2017-01-01

The efficient fermentative production of solvents (acetone, n-butanol, and ethanol) from a lignocellulosic feedstock using a single process microorganism has yet to be demonstrated. Herein, we developed a consolidated bioprocessing (CBP) based on a twin-clostridial consortium composed of Clostridium cellulovorans and Clostridium beijerinckii capable of producing cellulosic butanol from alkali-extracted, deshelled corn cobs (AECC). To accomplish this a genetic system was developed for C. cellulovorans and used to knock out the genes encoding acetate kinase (Clocel_1892) and lactate dehydrogenase (Clocel_1533), and to overexpress the gene encoding butyrate kinase (Clocel_3674), thereby pulling carbon flux towards butyrate production. In parallel, to enhance ethanol production, the expression of a putative hydrogenase gene (Clocel_2243) was down-regulated using CRISPR interference (CRISPRi). Simultaneously, genes involved in organic acids reassimilation (ctfAB, cbei_3833/3834) and pentose utilization (xylR, cbei_2385 and xylT, cbei_0109) were engineered in C. beijerinckii to enhance solvent production. The engineered twin-clostridia consortium was shown to decompose 83.2g/L of AECC and produce 22.1g/L of solvents (4.25g/L acetone, 11.5g/L butanol and 6.37g/L ethanol). This titer of acetone-butanol-ethanol (ABE) approximates to that achieved from a starchy feedstock. The developed twin-clostridial consortium serves as a promising platform for ABE fermentation from lignocellulose by CBP. Copyright © 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

Improvement of the butanol production selectivity and butanol to acetone ratio (B:A) by addition of electron carriers in the batch culture of a new local isolate of Clostridium acetobutylicum YM1.

PubMed

Nasser Al-Shorgani, Najeeb Kaid; Kalil, Mohd Sahaid; Wan Yusoff, Wan Mohtar; Shukor, Hafiza; Hamid, Aidil Abdul

2015-12-01

Improvement in the butanol production selectivity or enhanced butanol:acetone ratio (B:A) is desirable in acetone-butanol-ethanol (ABE) fermentation by Clostridium strains. In this study, artificial electron carriers were added to the fermentation medium of a new isolate of Clostridium acetobutylicum YM1 in order to improve the butanol yield and B:A ratio. The results revealed that medium supplementation with electron carriers changed the metabolism flux of electron and carbon in ABE fermentation by YM1. A decrease in acetone production, which subsequently improved the B:A ratio, was observed. Further improvement in the butanol production and B:A ratios were obtained when the fermentation medium was supplemented with butyric acid. The maximum butanol production (18.20 ± 1.38 g/L) was gained when a combination of methyl red and butyric acid was added. Although the addition of benzyl viologen (0.1 mM) and butyric acid resulted in high a B:A ratio of 16:1 (800% increment compared with the conventional 2:1 ratio), the addition of benzyl viologen to the culture after 4 h resulted in the production of 18.05 g/L butanol. Manipulating the metabolic flux to butanol through the addition of electron carriers could become an alternative strategy to achieve higher butanol productivity and improve the B:A ratio. Copyright © 2015 Elsevier Ltd. All rights reserved.

Recent advances and state-of-the-art strategies in strain and process engineering for biobutanol production by Clostridium acetobutylicum.

PubMed

Xue, Chuang; Zhao, Jingbo; Chen, Lijie; Yang, Shang-Tian; Bai, Fengwu

Butanol as an advanced biofuel has gained great attention due to its environmental benefits and superior properties compared to ethanol. However, the cost of biobutanol production via conventional acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum is not economically competitive, which has hampered its industrial application. The strain performance and downstream process greatly impact the economics of biobutanol production. Although various engineered strains with carefully orchestrated metabolic and sporulation-specific pathways have been developed, none of them is ideal for industrial biobutanol production. For further strain improvement, it is necessary to develop advanced genome editing tools and a deep understanding of cellular functioning of genes in metabolic and regulatory pathways. Processes with integrated product recovery can increase fermentation productivity by continuously removing inhibitory products while generating butanol (ABE) in a concentrated solution. In this review, we provide an overview of recent advances in C. acetobutylicum strain engineering and process development focusing on in situ product recovery. With deep understanding of systematic cellular bioinformatics, the exploration of state-of-the-art genome editing tools such as CRISPR-Cas for targeted gene knock-out and knock-in would play a vital role in Clostridium cell engineering for biobutanol production. Developing advanced hybrid separation processes for in situ butanol recovery, which will be discussed with a detailed comparison of advantages and disadvantages of various recovery techniques, is also imperative to the economical development of biobutanol. Copyright © 2017 Elsevier Inc. All rights reserved.

Synergistic dark and photo-fermentation continuous system for hydrogen production from molasses by Clostridium acetobutylicum ATCC 824 and Rhodobacter capsulatus DSM 1710.

PubMed

Morsy, Fatthy Mohamed

2017-04-01

This study investigated synergistic dark and photo-fermentation using continuous fermentation system (CFS). The system relies on connecting several fermenters from bottom of one to top culture level of the next in a manner that allows for delaying movement of the substrate and thus for its full consumption. While H 2 was collected, CFS allowed for moving liquid byproducts toward the outlet and hence continuous productivity. CFS could be efficiently used for: (1) Continuous dark and photo-fermentation H 2 production by Clostridium acetobutylicum and Rhodobacter capsulatus producing 5.65moleH 2 mole -1 hexose; (2) Continuous dark-fermentation synergistic H 2 , acetone, butanol and ethanol (ABE) production by C. acetobutylicum which produced per mole hexose, 2.43mol H 2 along with 73.08g ABE (3) Continuous H 2 and methane production by C. acetobutylicum and bacterial sludge producing, per mole hexose, 1.64mol pure H 2 and 2.56mol CH 4 mixed with 0.37mol H 2 ·The hydraulic retention time (HRT) for whole system was short where organic acids produced in dark-fermentation in first fermenter were synergistically utilized for H 2 production by R. capsulatus in subsequent fermenters. CFS is suitable for fast-digestible sugars but not lignocelluloses or other hard-digestible organics, requiring prolonged HRT, unless such polymeric organics were hydrolyzed prior to fermentation. Copyright © 2017 Elsevier B.V. All rights reserved.

Privacy-Preserving and Secure Sharing of PHR in the Cloud.

PubMed

Zhang, Leyou; Wu, Qing; Mu, Yi; Zhang, Jingxia

2016-12-01

As a new summarized record of an individual's medical data and information, Personal Health Record (PHR) can be accessible online. The owner can control fully his/her PHR files to be shared with different users such as doctors, clinic agents, and friends. However, in an open network environment like in the Cloud, these sensitive privacy information may be gotten by those unauthorized parties and users. In this paper, we consider how to achieve PHR data confidentiality and provide fine-grained access control of PHR files in the public Cloud based on Attribute Based Encryption(ABE). Differing from previous works, we also consider the privacy preserving of the receivers since the attributes of the receivers relate to their identity or medical information, which would make some sensitive data exposed to third services. Anonymous ABE(AABE) not only enforces the security of PHR of the owners but also preserves the privacy of the receivers. But a normal AABE with a single private key generation(PKG) center may not match a PHR system in the hierarchical architecture. Therefore, we discuss not only the construction of the PHR sharing system base on AABE but also how to construct the PHR sharing system based on the hierarchical AABE. The proposed schemes(especially based on hierarchical AABE) have many advantages over the available such as short public keys, constant-size private keys, which overcome the weaknesses in the existing works. In the standard model, the introduced schemes achieve compact security in the prime order groups.

Enhancing Navigation Skills through Audio Gaming.

PubMed

Sánchez, Jaime; Sáenz, Mauricio; Pascual-Leone, Alvaro; Merabet, Lotfi

2010-01-01

We present the design, development and initial cognitive evaluation of an Audio-based Environment Simulator (AbES). This software allows a blind user to navigate through a virtual representation of a real space for the purposes of training orientation and mobility skills. Our findings indicate that users feel satisfied and self-confident when interacting with the audio-based interface, and the embedded sounds allow them to correctly orient themselves and navigate within the virtual world. Furthermore, users are able to transfer spatial information acquired through virtual interactions into real world navigation and problem solving tasks.

Cultural Resources Sample Survey of the Bayou Cocodrie and Tributaries Project, St. Landry, Evangeline and Avoyelles Parishes, Louisiana.

DTIC Science & Technology

1987-02-06

noweinv m idensitir 6F block ntiAbe) - This report presents the results of a probabilistic survey T of five per cent f -the wooded areas within the...bottle necks, and one piece of slag . The lack of structural remains, such as bricks or nails, 105 THIS PAGE BLANK 106 0 loom IWO I > - MIRE SITE .q...melted 1 Slag 1 Total 26 146 CHAPTER VII A COMPARISON OF SITE DISTRIBUTIONS IN THE PROJECT AREA Using the data gathered during this survey, comparisons

Crystal Chemistry, Magnetic and Electrical Properties of La(2-X)BaXNiO4

DTIC Science & Technology

1989-02-01

alternating rock-salt, AO, and perovskite , ABe 3 , layers, with the separation between layers being almost twice the intraplanar distance between two B...t c 1.02, and it is most stable for t - 1.0. The 2 factor arises because the {110 planes of the perovskite layers are stacked alternately with the...a result of Ba 2 + substitution for La3 +.° This is easily understood with respect to the K2 NiF4 structure. The alternating perovskite and rock-salt

Entropic characterization of separability in Gaussian states

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sudha; Devi, A. R. Usha; Inspire Institute Inc., McLean, Virginia 22101

2010-02-15

We explore separability of bipartite divisions of mixed Gaussian states based on the positivity of the Abe-Rajagopal (AR) q-conditional entropy. The AR q-conditional entropic characterization provide more stringent restrictions on separability (in the limit q{yields}{infinity}) than that obtained from the corresponding von Neumann conditional entropy (q=1 case)--similar to the situation in finite dimensional states. Effectiveness of this approach, in relation to the results obtained by partial transpose criterion, is explicitly analyzed in three illustrative examples of two-mode Gaussian states of physical significance.

Molecular Cloning and Function of FAS/APO1 Associated Protein in Breast Cancer.

DTIC Science & Technology

1996-06-01

Ariyama T, Abe T, Druck T, Ohta M, Huebner K, Yanagisawa J, Reed JC, Sato T: PTPN13, a Fas-associated protein tyrosine phosphatase, is located on...20. Yang, Q., and Tonks, N. K. (1991). Isolation of a cDNA clone encoding a human protein-tyrosine phosphatase with homology 7. Huebner, K., Druck , T...Acad. Sci. U.S.A. 91, 7477 (1994). Res. 53, 1945 (1993).(Fig. 3D ). In contrast to Jurkat cells which 13. The original description of PTP-BAS (12

Enhancing Navigation Skills through Audio Gaming

PubMed Central

Sánchez, Jaime; Sáenz, Mauricio; Pascual-Leone, Alvaro; Merabet, Lotfi

2014-01-01

We present the design, development and initial cognitive evaluation of an Audio-based Environment Simulator (AbES). This software allows a blind user to navigate through a virtual representation of a real space for the purposes of training orientation and mobility skills. Our findings indicate that users feel satisfied and self-confident when interacting with the audio-based interface, and the embedded sounds allow them to correctly orient themselves and navigate within the virtual world. Furthermore, users are able to transfer spatial information acquired through virtual interactions into real world navigation and problem solving tasks. PMID:25505796

FT-IR spectroscopic analysis for studying Clostridium cell response to conversion of enzymatically hydrolyzed hay

NASA Astrophysics Data System (ADS)

Grube, Mara; Gavare, Marita; Nescerecka, Alina; Tihomirova, Kristina; Mezule, Linda; Juhna, Talis

2013-07-01

Grass hay is one of assailable cellulose containing non-food agricultural wastes that can be used as a carbohydrate source by microorganisms producing biofuels. In this study three Clostridium strains Clostridium acetobutylicum, Clostridium beijerinckii and Clostridium tetanomorphum, capable of producing acetone, butanol and ethanol (ABE) were adapted to convert enzymatically hydrolyzed hay used as a growth media additive. The results of growth curves, substrate degradation kinetics and FT-IR analyses of bacterial biomass macromolecular composition showed diverse strain-specific cell response to the growth medium composition.

Novel clostridial fusants in comparison with co-cultured counterpart species for enhanced production of biobutanol using green renewable and sustainable feedstock.

PubMed

Syed, Kashif; Dahman, Yaser

2015-11-01

In this work, biobutanol was produced through simultaneous saccharification and fermentation (SSF) of wheat straw (WS) that traditionally produces acetone, butanol and ethanol solvents (ABE). Thermal stability was imparted to two mesophilic clostridial wild strains (Clostridium beijerinckii and Clostridium acetobutylicum) through protoplast fusion with that of a corresponding thermophilic clostridial species (Clostridium thermocellum). Production was pursued by the fused strains at 45 °C compared to that of the corresponding co-cultures at 35 °C. Results showed that the fused strains generally achieved higher production at 45 °C than that of the corresponding co-cultures at 35 °C. Highest butanol production of 13.82 g/L was recorded with C. beijerinckii fusant, with ABE solvents production of 23 g/L (yields of 0.17 and 0.57, respectively). Total sugar consumption of this strain was the highest among all strains and was 84%. Fused strains also showed immense level of tolerance towards butanol toxicity compared to the wild strains. Filter paper enzyme assay demonstrated that fused strains were able to produce cellulolytic enzymes in the range of 58.73-68.52 FPU/ml. Cellulosome producing C. thermocellum and its ability to ferment sugars offers a promising future in biofuels through eliminating the need to add external enzymes. Generally, productions reported in the present study were higher than literature where biobutanol stripping systems were employed to eliminate toxicity during production. This demonstrates a clear potential for improving productivity and yield at a larger-scale facility.

The Draft Genome Sequence of Clostridium sp. Strain NJ4, a Bacterium Capable of Producing Butanol from Inulin Through Consolidated Bioprocessing.

PubMed

Jiang, Yujia; Lu, Jiasheng; Chen, Tianpeng; Yan, Wei; Dong, Weiliang; Zhou, Jie; Zhang, Wenming; Ma, Jiangfeng; Jiang, Min; Xin, Fengxue

2018-05-23

A novel butanogenic Clostridium sp. NJ4 was successfully isolated and characterized, which could directly produce relatively high titer of butanol from inulin through consolidated bioprocessing (CBP). The assembled draft genome of strain NJ4 is 4.09 Mp, containing 3891 encoded protein sequences with G+C content of 30.73%. Among these annotated genes, a levanase, a hypothetical inulinase, and two bifunctional alcohol/aldehyde dehydrogenases (AdhE) were found to play key roles in the achievement of ABE production from inulin through CBP.

Separability of a family of one-parameter W and Greenberger-Horne-Zeilinger multiqubit states using the Abe-Rajagopal q-conditional-entropy approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prabhu, R.; Usha Devi, A. R.; Inspire Institute Inc., McLean, Virginia 22101

2007-10-15

We employ conditional Tsallis q entropies to study the separability of symmetric one parameter W and GHZ multiqubit mixed states. The strongest limitation on separability is realized in the limit q{yields}{infinity}, and is found to be much superior to the condition obtained using the von Neumann conditional entropy (q=1 case). Except for the example of two qubit and three qubit symmetric states of GHZ family, the q-conditional entropy method leads to sufficient--but not necessary--conditions on separability.

Capturing the response of Clostridium acetobutylicum to chemical stressors using a regulated genome-scale metabolic model

DOE PAGES

Dash, Satyakam; Mueller, Thomas J.; Venkataramanan, Keerthi P.; ...

2014-10-14

Clostridia are anaerobic Gram-positive Firmicutes containing broad and flexible systems for substrate utilization, which have been used successfully to produce a range of industrial compounds. Clostridium acetobutylicum has been used to produce butanol on an industrial scale through acetone-butanol-ethanol (ABE) fermentation. A genome-scale metabolic (GSM) model is a powerful tool for understanding the metabolic capacities of an organism and developing metabolic engineering strategies for strain development. The integration of stress related specific transcriptomics information with the GSM model provides opportunities for elucidating the focal points of regulation.