Sample records for einsteinium 246

  1. Einsteinium

    NASA Astrophysics Data System (ADS)

    Haire, Richard G.

    The discovery of einsteinium, element 99, came about during the analyses of nuclear products produced in and then recovered from test debris following a thermonuclear explosion (weapon test device, ‘Mike', November 1952) at Eniwetok Atoll in the Pacific Ocean. The uranium present in this device was subjected to a very intense neutron flux (integrated fluence of about 1024neutrons) in an extremely short time frame (few nanoseconds), which allowed a large number of multiple neutron captures with a minimal degree of decay of the products formed. Nuclei were formed with usually high neutron/proton ratios (very ‘heavy' uranium isotopes), which then rapidly beta-decayed into new, transuranium isotopes through element 100. Scientists from several U.S. Government laboratories separated and analyzed extensively the debris samplings in the following weeks. From these investigations came the discovery and identification of einsteinium and fermium. The first element was named in honor of Albert Einstein, and assigned the symbol, E (later changed to the current symbol, Es). Additional details and discussions about the discovery of this element and the scientists involved are given in several references (Thompson et al., 1954; Ghiorso et al., 1955; Fields et al., 1956; Hyde et al., 1964; Seaborg and Loveland, 1990).

  2. Chemical Properties of Elements 99 and 100 [Einsteinium and Fermium

    DOE R&D Accomplishments Database

    Seaborg, G. T.; Thompson, S. G.; Harvey, B. G.; Choppin, G. R.

    1954-07-23

    A description of some of the chemical properties and of the methods used in the separations of elements 99 [Einsteinium] and 100 [Fermium] are given. The new elements exhibit the properties expected for the tenth and eleventh actinide elements. Attempts to produce an oxidation state greater than III of element 99 have been unsuccessful. In normal aqueous media only the III state of element 100 appears to exist. The relative spacings of the elution peaks of the new elements in some separations with ion exchange resin columns are the same as the relative spacings of the homologous lanthanide elements. The results of experiments involving cation exchange resins with very concentrated hydrochloric acid eluant show that the new elements, like the earlier actinides, are more strongly complexed than the lanthanides. The new elements also exist partially as anions in concentrated hydrochloric acid, as do earlier actinide elements, and they may be partially separated from each other by means of ion exchange resins. With some eluants interesting reversals of elution positions are observed in the region Bk-Cf-99-100, indicating complex ion formation involving unusual factors.

  3. BAG PASSOUT SEALER FOR WATER-SHIELDED CAVE FACILITY (Engineering Materials)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    1963-10-31

    The water-shielded cave facility is used in processing irradiated slugs for recovery of americium, curium, berkelium, californium, einsteinium, and fermium. The remotely operated, plastic-bag passout sealer is used in removing isotopic fractions for storage in the rear or for removing radioactive waste for placement in the waste storage containers. The unit is accessible by both the primary inclosure master-slaves and the service area master-slaves. (F.L.S.)

  4. Handbook of Basic Atomic Spectroscopic Data

    National Institute of Standards and Technology Data Gateway

    SRD 108 Handbook of Basic Atomic Spectroscopic Data (Web, free access)   This handbook provides a selection of the most important and frequently used atomic spectroscopic data. The compilation includes data for the neutral and singly-ionized atoms of all elements hydrogen through einsteinium (Z = 1-99). The wavelengths, intensities, and spectrum assignments are given for each element, and the data for the approximately 12,000 lines of all elements are also collected into a single table.

  5. Systematization of actinides using cluster analysis

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kopyrin, A.A.; Terent`eva, T.N.; Khramov, N.N.

    1994-11-01

    A representation of the actinides in multidimensional property space is proposed for systematization of these elements using cluster analysis. Literature data for their atomic properties are used. Owing to the wide variation of published ionization potentials, medians are used to estimate them. Vertical dendograms are used for classification on the basis of distances between the actinides in atomic-property space. The properties of actinium and lawrencium are furthest removed from the main group. Thorium and mendelevium exhibit individualized properties. A cluster based on the einsteinium-fermium pair is joined by californium.

  6. Contribution of filamentous fungi to the musty odorant 2,4,6-trichloroanisole in water supply reservoirs and associated drinking water treatment plants.

    PubMed

    Bai, Xiuzhi; Zhang, Ting; Qu, Zhipeng; Li, Haipu; Yang, Zhaoguang

    2017-09-01

    In this study, the distribution of 2,4,6-trichloroanisole (2,4,6-TCA) in two water supply reservoirs and four associated drinking water treatment plants (DWTPs) were investigated. The 2,4,6-TCA concentrations were in the range of 1.53-2.36 ng L -1 in water supply reservoirs and 0.76-6.58 ng L -1 at DWTPs. To determine the contribution of filamentous fungi to 2,4,6-TCA in a full-scale treatment process, the concentrations of 2,4,6-TCA in raw water, settled water, post-filtration water, and finished water were measured. The results showed that 2,4,6-TCA levels continuously increased until chlorination, suggesting that 2,4,6-TCA could form without a chlorination reaction and fungi might be the major contributor to the 2,4,6-TCA formation. Meanwhile, twenty-nine fungal strains were isolated and identified by morphological and molecular biological methods. Of the seventeen isolated fungal species, eleven showed the capability to convert 2,4,6-trichlorophenol (2,4,6-TCP) to 2,4,6-TCA. The highest level of 2,4,6-TCA formation was carried out by Aspergillus versicolor voucher BJ1-3: 40.5% of the original 2,4,6-TCP was converted to 2,4,6-TCA. There was a significant variation in the capability of different species to generate 2,4,6-TCA. The results from the proportions of cell-free, cell-attached, and cell-bound 2,4,6-TCA suggested that 2,4,6-TCA generated by fungi was mainly distributed in their extracellular environment. In addition to 2,4,6-TCA, five putative volatile by-products were also identified by gas chromatography and mass spectrometry. These findings increase our understanding on the mechanisms involved in the formation of 2,4,6-TCA and provide insights into managing and controlling 2,4,6-TCA-related problems in drinking water. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. 48 CFR 246.371 - Notification of potential safety issues.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... safety issues. 246.371 Section 246.371 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 246.371 Notification of potential safety issues. (a) Use the clause at 252.246-7003, Notification of Potential Safety...

  8. 48 CFR 252.246-7002 - Warranty of construction (Germany).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... (Germany). 252.246-7002 Section 252.246-7002 Federal Acquisition Regulations System DEFENSE ACQUISITION... of Provisions And Clauses 252.246-7002 Warranty of construction (Germany). As prescribed in 246.710(4), use the following clause: Warranty of Construction (Germany) (JUN 1997) (a) In addition to any other...

  9. 48 CFR 252.246-7002 - Warranty of construction (Germany).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... (Germany). 252.246-7002 Section 252.246-7002 Federal Acquisition Regulations System DEFENSE ACQUISITION... of Provisions And Clauses 252.246-7002 Warranty of construction (Germany). As prescribed in 246.710(4), use the following clause: Warranty of Construction (Germany) (JUN 1997) (a) In addition to any other...

  10. 48 CFR 252.246-7002 - Warranty of construction (Germany).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... (Germany). 252.246-7002 Section 252.246-7002 Federal Acquisition Regulations System DEFENSE ACQUISITION... of Provisions And Clauses 252.246-7002 Warranty of construction (Germany). As prescribed in 246.710(4), use the following clause: Warranty of Construction (Germany) (JUN 1997) (a) In addition to any other...

  11. 48 CFR 252.246-7002 - Warranty of construction (Germany).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... (Germany). 252.246-7002 Section 252.246-7002 Federal Acquisition Regulations System DEFENSE ACQUISITION... of Provisions And Clauses 252.246-7002 Warranty of construction (Germany). As prescribed in 246.710(2), use the following clause: Warranty of Construction (Germany) (JUN 1997) (a) In addition to any other...

  12. 48 CFR 252.246-7002 - Warranty of construction (Germany).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... (Germany). 252.246-7002 Section 252.246-7002 Federal Acquisition Regulations System DEFENSE ACQUISITION... of Provisions And Clauses 252.246-7002 Warranty of construction (Germany). As prescribed in 246.710(4), use the following clause: Warranty of Construction (Germany) (JUN 1997) (a) In addition to any other...

  13. 48 CFR 252.246-7005 - Notice of Warranty Tracking of Serialized Items.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Tracking of Serialized Items. 252.246-7005 Section 252.246-7005 Federal Acquisition Regulations System... AND CONTRACT CLAUSES Text of Provisions And Clauses 252.246-7005 Notice of Warranty Tracking of Serialized Items. As prescribed in 246.710(5)(i)(A), use the following provision: Notice of Warranty Tracking...

  14. Cork Taint of Wines: Role of the Filamentous Fungi Isolated from Cork in the Formation of 2,4,6-Trichloroanisole by O Methylation of 2,4,6-Trichlorophenol

    PubMed Central

    Álvarez-Rodríguez, María Luisa; López-Ocaña, Laura; López-Coronado, José Miguel; Rodríguez, Enrique; Martínez, María Jesús; Larriba, Germán; Coque, Juan-José R.

    2002-01-01

    Cork taint is a musty or moldy off-odor in wine mainly caused by 2,4,6-trichloroanisole (2,4,6-TCA). We examined the role of 14 fungal strains isolated from cork samples in the production of 2,4,6-TCA by O methylation of 2,4,6-trichlorophenol (2,4,6-TCP). The fungal strains isolated belong to the genera Penicillium (four isolates); Trichoderma (two isolates); and Acremonium, Chrysonilia, Cladosporium, Fusarium, Mortierella, Mucor, Paecilomyces, and Verticillium (one isolate each). Eleven of these strains could produce 2,4,6-TCA when they were grown directly on cork in the presence of 2,4,6-TCP. The highest levels of bioconversion were carried out by the Trichoderma and Fusarium strains. One strain of Trichoderma longibrachiatum could also efficiently produce 2,4,6-TCA in liquid medium. However, no detectable levels of 2,4,6-TCA production by this strain could be detected on cork when putative precursors other than 2,4,6-TCP, including several anisoles, dichlorophenols, trichlorophenols, or other highly chlorinated compounds, were tested. Time course expression studies with liquid cultures showed that the formation of 2,4,6-TCA was not affected by a high concentration of glucose (2% or 111 mM) or by ammonium salts at concentrations up to 60 mM. In T. longibrachiatum the O methylation of 2,4,6-TCP was catalyzed by a mycelium-associated S-adenosyl-l-methionine (SAM)-dependent methyltransferase that was strongly induced by 2,4,6-TCP. The reaction was inhibited by S-adenosyl-l-homocysteine, an inhibitor of SAM-dependent methylation, suggesting that SAM is the natural methyl donor. These findings increase our understanding of the mechanism underlying the origin of 2,4,6-TCA on cork, which is poorly understood despite its great economic importance for the wine industry, and they could also help us improve our knowledge about the biodegradation and detoxification processes associated with chlorinated phenols. PMID:12450804

  15. Degradation of 2,4,6-Trichlorophenol and hydrogen production simultaneously by TiO2 nanotubes/graphene composite

    NASA Astrophysics Data System (ADS)

    Slamet, Raudina

    2017-11-01

    Industrial waters in coal pyrolysis process, synthetic chemicals and oil and gas process contain phenol derivatives that are dangerous to the environment and needs to be removed, one of them is 2,4,6-Trichlorophenol. Degradation of 2,4,6-Trichlorophenol and hydrogen production simultaneously have been investigated using TiNT/Graphene composite at various graphene loading and initial concentration of 2,4,6-Trichlorophenol. Optimal graphene loading of 0.6 wt% was obtained in the simultaneous system with 89% elimination of 2,4,6-Trichlorophenol and 986 µmol of hydrogen production. Test results showed that addition of 2,4,6-Trichlorophenol would subsequently increased 2,4,6-Trichlorophenol conversion and enhanced hydrogen production linearly. 2.7 times greater hydrogen production was found in addition of 50 ppm 2,4,6-Trichlorophenol.

  16. 48 CFR 246.470 - Government contract quality assurance actions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... quality assurance actions. 246.470 Section 246.470 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.470 Government contract quality assurance actions. ...

  17. 48 CFR 246.705 - Limitations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Limitations. 246.705 Section 246.705 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Warranties 246.705 Limitations. (a) In addition to...

  18. 48 CFR 2453.246 - Quality Assurance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Quality Assurance. 2453.246 Section 2453.246 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT CLAUSES AND FORMS FORMS Prescription of Forms 2453.246 Quality Assurance. ...

  19. 48 CFR 246.408 - Single-agency assignments of Government contract quality assurance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of Government contract quality assurance. 246.408 Section 246.408 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.408 Single-agency assignments of Government contract...

  20. 48 CFR 246.504 - Certificate of conformance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Certificate of conformance. 246.504 Section 246.504 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Acceptance 246.504 Certificate of conformance...

  1. 48 CFR 246.701 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....246-19, Warranty of Systems and Equipment Under Performance Specifications or Design Criteria; and FAR... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Definitions. 246.701 Section 246.701 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT...

  2. 48 CFR 246.710-70 - Warranty attachment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Warranty attachment. 246.710-70 Section 246.710-70 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Warranties 246.710-70 Warranty...

  3. 48 CFR 246.710-70 - Warranty attachment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Warranty attachment. 246.710-70 Section 246.710-70 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Warranties 246.710-70 Warranty...

  4. 48 CFR 246.710-70 - Warranty attachment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Warranty attachment. 246.710-70 Section 246.710-70 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Warranties 246.710-70 Warranty...

  5. 40 CFR 246.202-1 - Requirement.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Requirement. 246.202-1 Section 246.202-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR MATERIALS RECOVERY GUIDELINES Requirements and Recommended Procedures § 246.202-1 Requirement...

  6. 40 CFR 246.200-1 - Requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Requirements. 246.200-1 Section 246.200-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR MATERIALS RECOVERY GUIDELINES Requirements and Recommended Procedures § 246.200-1 Requirements...

  7. 40 CFR 246.201-1 - Requirement.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Requirement. 246.201-1 Section 246.201-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR MATERIALS RECOVERY GUIDELINES Requirements and Recommended Procedures § 246.201-1 Requirement...

  8. 40 CFR 246.202-1 - Requirement.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Requirement. 246.202-1 Section 246.202-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR MATERIALS RECOVERY GUIDELINES Requirements and Recommended Procedures § 246.202-1 Requirement...

  9. 40 CFR 246.201-1 - Requirement.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Requirement. 246.201-1 Section 246.201-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR MATERIALS RECOVERY GUIDELINES Requirements and Recommended Procedures § 246.201-1 Requirement...

  10. 40 CFR 246.202-1 - Requirement.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Requirement. 246.202-1 Section 246.202-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR MATERIALS RECOVERY GUIDELINES Requirements and Recommended Procedures § 246.202-1 Requirement...

  11. 40 CFR 246.200-1 - Requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Requirements. 246.200-1 Section 246.200-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR MATERIALS RECOVERY GUIDELINES Requirements and Recommended Procedures § 246.200-1 Requirements...

  12. 40 CFR 246.200-1 - Requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Requirements. 246.200-1 Section 246.200-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR MATERIALS RECOVERY GUIDELINES Requirements and Recommended Procedures § 246.200-1 Requirements...

  13. 40 CFR 246.201-1 - Requirement.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Requirement. 246.201-1 Section 246.201-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR MATERIALS RECOVERY GUIDELINES Requirements and Recommended Procedures § 246.201-1 Requirement...

  14. 48 CFR 246.706 - Warranty terms and conditions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Warranty terms and conditions. 246.706 Section 246.706 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Warranties 246.706 Warranty terms and...

  15. 48 CFR 246.202-4 - Higher-level contract quality requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... quality requirements. 246.202-4 Section 246.202-4 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 246.202-4 Higher-level contract quality requirements. (1) Higher-level contract quality...

  16. 48 CFR 52.246-14 - Inspection of Transportation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Transportation. 52.246-14 Section 52.246-14 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION....246-14 Inspection of Transportation. As prescribed in 46.314, insert the following clause in solicitations and contracts for freight transportation services (including local drayage) by rail, motor...

  17. 48 CFR 52.246-14 - Inspection of Transportation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Transportation. 52.246-14 Section 52.246-14 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION....246-14 Inspection of Transportation. As prescribed in 46.314, insert the following clause in solicitations and contracts for freight transportation services (including local drayage) by rail, motor...

  18. 48 CFR 52.246-14 - Inspection of Transportation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Transportation. 52.246-14 Section 52.246-14 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION....246-14 Inspection of Transportation. As prescribed in 46.314, insert the following clause in solicitations and contracts for freight transportation services (including local drayage) by rail, motor...

  19. 48 CFR 52.246-14 - Inspection of Transportation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Transportation. 52.246-14 Section 52.246-14 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION....246-14 Inspection of Transportation. As prescribed in 46.314, insert the following clause in solicitations and contracts for freight transportation services (including local drayage) by rail, motor...

  20. 32 CFR 246.2 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Applicability. 246.2 Section 246.2 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.2 Applicability. This part applies to...

  1. 32 CFR 246.7 - Information requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Information requirements. 246.7 Section 246.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.7 Information requirements. The...

  2. 32 CFR 246.7 - Information requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Information requirements. 246.7 Section 246.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.7 Information requirements. The...

  3. 32 CFR 246.2 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Applicability. 246.2 Section 246.2 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.2 Applicability. This part applies to...

  4. 32 CFR 246.2 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 2 2011-07-01 2011-07-01 false Applicability. 246.2 Section 246.2 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.2 Applicability. This part applies to...

  5. 32 CFR 246.2 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Applicability. 246.2 Section 246.2 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.2 Applicability. This part applies to...

  6. 32 CFR 246.7 - Information requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Information requirements. 246.7 Section 246.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.7 Information requirements. The...

  7. 32 CFR 246.7 - Information requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 2 2011-07-01 2011-07-01 false Information requirements. 246.7 Section 246.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.7 Information requirements. The...

  8. 32 CFR 246.7 - Information requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Information requirements. 246.7 Section 246.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.7 Information requirements. The...

  9. 32 CFR 246.2 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Applicability. 246.2 Section 246.2 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.2 Applicability. This part applies to...

  10. 48 CFR 246.370 - Material inspection and receiving report.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... deliverable is a scientific or technical report; (5) Research and development contracts not requiring the... receiving report. 246.370 Section 246.370 Federal Acquisition Regulations System DEFENSE ACQUISITION... Material inspection and receiving report. (a) Use the clause at 252.246-7000, Material Inspection and...

  11. 48 CFR 246.370 - Material inspection and receiving report.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... deliverable is a scientific or technical report; (5) Research and development contracts not requiring the... receiving report. 246.370 Section 246.370 Federal Acquisition Regulations System DEFENSE ACQUISITION... Material inspection and receiving report. (a) Use the clause at 252.246-7000, Material Inspection and...

  12. 40 CFR 246.202-5 - Recommended procedures: Transportation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Recommended procedures: Transportation. 246.202-5 Section 246.202-5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.202-5 Recommended procedures: Transportation. Transportation to market may be supplied by either...

  13. 40 CFR 246.202-5 - Recommended procedures: Transportation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Transportation. 246.202-5 Section 246.202-5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.202-5 Recommended procedures: Transportation. Transportation to market may be supplied by either...

  14. 40 CFR 246.202-5 - Recommended procedures: Transportation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Recommended procedures: Transportation. 246.202-5 Section 246.202-5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.202-5 Recommended procedures: Transportation. Transportation to market may be supplied by either...

  15. 40 CFR 246.200-7 - Recommended procedures: Transportation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Recommended procedures: Transportation. 246.200-7 Section 246.200-7 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.200-7 Recommended procedures: Transportation. Transportation to market may be supplied by the...

  16. 40 CFR 246.202-5 - Recommended procedures: Transportation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Recommended procedures: Transportation. 246.202-5 Section 246.202-5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.202-5 Recommended procedures: Transportation. Transportation to market may be supplied by either...

  17. 40 CFR 246.200-7 - Recommended procedures: Transportation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Recommended procedures: Transportation. 246.200-7 Section 246.200-7 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.200-7 Recommended procedures: Transportation. Transportation to market may be supplied by the...

  18. 40 CFR 246.200-7 - Recommended procedures: Transportation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Transportation. 246.200-7 Section 246.200-7 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.200-7 Recommended procedures: Transportation. Transportation to market may be supplied by the...

  19. 40 CFR 246.200-7 - Recommended procedures: Transportation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Recommended procedures: Transportation. 246.200-7 Section 246.200-7 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.200-7 Recommended procedures: Transportation. Transportation to market may be supplied by the...

  20. 7 CFR 246.13 - Financial management system.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 4 2014-01-01 2014-01-01 false Financial management system. 246.13 Section 246.13... State Agency Provisions § 246.13 Financial management system. (a) Disclosure of expenditures. The State agency shall maintain a financial management system which provides accurate, current and complete...

  1. 7 CFR 246.13 - Financial management system.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 4 2013-01-01 2013-01-01 false Financial management system. 246.13 Section 246.13... State Agency Provisions § 246.13 Financial management system. (a) Disclosure of expenditures. The State agency shall maintain a financial management system which provides accurate, current and complete...

  2. 7 CFR 246.13 - Financial management system.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 4 2012-01-01 2012-01-01 false Financial management system. 246.13 Section 246.13... State Agency Provisions § 246.13 Financial management system. (a) Disclosure of expenditures. The State agency shall maintain a financial management system which provides accurate, current and complete...

  3. 7 CFR 246.13 - Financial management system.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Financial management system. 246.13 Section 246.13... State Agency Provisions § 246.13 Financial management system. (a) Disclosure of expenditures. The State agency shall maintain a financial management system which provides accurate, current and complete...

  4. Aerobic secondary utilization of a non-growth and inhibitory substrate 2,4,6-trichlorophenol by Sphingopyxis chilensis S37 and sphingopyxis-like strain S32.

    PubMed

    Aranda, Carlos; Godoy, Félix; Becerra, José; Barra, Ricardo; Martínez, Miguel

    2003-08-01

    This paper reports 2,4,6-trichlorophenol (246TCP) degradation by Sphingopyxis chilensis S37 and Sphingopyxis chilensis-like strain S32, which were unable to use 246TCP as the sole carbon and energy source. In R2A broth, the strains degraded 246TCP up to 0.5 mM. Results with mixtures of different 246TCP and glucose concentrations in mineral salt media demonstrated dependence on glucose to allow bacterial growth and degradation of 246TCP. Strain S32 degraded halophenol up to 0.2 mM when 5.33 mM glucose was simultaneously added, while strain S37 degraded the compound up to 0.1 mM when 1.33 mM glucose was added. These 246TCP concentrations were lethal for inocula in absence of glucose. Stoichiometric releases of chloride and analysis by HPLC, GC-ECD and GC-MS indicated 246TCP mineralisation by both strains. To our knowledge, this is the first report of bacteria able to mineralize a chlorophenol as a non-growth and inhibitory substrate. The concept of secondary utilization instead of cometabolism is proposed for this activity.

  5. 37 CFR 2.46 - Principal Register.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Principal Register. 2.46 Section 2.46 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES The Written Application § 2.46 Principal Register. All...

  6. 48 CFR 552.246-71 - Source Inspection by Government.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Government. 552.246-71 Section 552.246-71 Federal Acquisition Regulations System GENERAL SERVICES....246-71 Source Inspection by Government. As prescribed in 546.302-71, insert the following clause: Source Inspection by Government (JUNE 1, 2009) (a) Inspection by Government personnel. (1) Supplies to be...

  7. 48 CFR 246.701 - Definitions as used in this subpart.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Nature; FAR 52.246-19, Warranty of Systems and Equipment Under Performance Specifications or Design... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Definitions as used in this subpart. 246.701 Section 246.701 Federal Acquisition Regulations System DEFENSE ACQUISITION...

  8. 48 CFR 52.246-3 - Inspection of Supplies-Cost-Reimbursement.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-Cost-Reimbursement. 52.246-3 Section 52.246-3 Federal Acquisition Regulations System FEDERAL... Provisions and Clauses 52.246-3 Inspection of Supplies—Cost-Reimbursement. As prescribed in 46.303, insert... furnishing of supplies, when a cost-reimbursement contract is contemplated: Inspection of Supplies—Cost...

  9. 40 CFR 246.201-6 - Recommended procedures: Transportation to market.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recommended procedures: Transportation to market. 246.201-6 Section 246.201-6 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Procedures § 246.201-6 Recommended procedures: Transportation to market. Transportation to market may be...

  10. 42 CFR 431.246 - Corrective action.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 4 2011-10-01 2011-10-01 false Corrective action. 431.246 Section 431.246 Public... Recipients Procedures § 431.246 Corrective action. The agency must promptly make corrective payments, retroactive to the date an incorrect action was taken, and, if appropriate, provide for admission or...

  11. 42 CFR 431.246 - Corrective action.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Corrective action. 431.246 Section 431.246 Public... Recipients Procedures § 431.246 Corrective action. The agency must promptly make corrective payments, retroactive to the date an incorrect action was taken, and, if appropriate, provide for admission or...

  12. 2,4,6-Trichlorophenol cytotoxicity involves oxidative stress, endoplasmic reticulum stress, and apoptosis.

    PubMed

    Zhang, Xiaoning; Zhang, Xiaona; Niu, Zhidan; Qi, Yongmei; Huang, Dejun; Zhang, Yingmei

    2014-01-01

    This study aims to evaluate the cytotoxicity and potential mechanisms of 2,4,6-trichlorophenol (2,4,6-TCP) in mouse embryonic fibroblasts. Our results show that 2,4,6-TCP causes morphological changes and reduces cell viability. The overproduction of reactive oxygen species, the upregulation of nuclear factor-E2-related factor 2 (Nrf2) and heme oxygenase 1 (HMOX1) messenger RNA (mRNA) expressions, and the nuclear translocation of Nrf2 protein demonstrate that 2,4,6-TCP induces oxidative stress, and the Nrf2/HMOX1 pathway might be involved in 2,4,6-TCP-induced antioxidative response. Simultaneously, our data also demonstrate that 2,4,6-TCP upregulates the expressions of binding immunoglobulin protein, inositol-requiring enzyme/endonuclease 1α, and C/EBP homologous protein; stimulates α subunit of eukaryotic translation initiation factor 2 phosphorylation; and induces the splicing of Xbp1 mRNA, suggesting that endoplasmic reticulum (ER) stress is triggered. Moreover, 2,4,6-TCP alters the mitochondrial membrane potential and increases the apoptosis rate, the caspase 3 activity, and the Bax/Bcl-2 ratio, demonstrating that the mitochondrial pathway is involved in the 2,4,6-TCP-induced apoptosis. Thus, these results show that 2,4,6-TCP induces oxidative stress, ER stress, and apoptosis, which together contribute to its cytotoxicity in vitro. © The Author(s) 2014.

  13. Role of UV photolysis in accelerating the biodegradation of 2,4,6-TCP.

    PubMed

    Wang, Wenbing; Kirumba, George; Zhang, Yongming; Wu, Yanqing; Rittmann, Bruce E

    2015-09-18

    2,4,6-TCP, a kind of chlorinated aromatic and aliphatic compound, is difficult to be biodegraded by ordinary microorganisms. UV photolysis and biodegradation of 2,4,6-TCP by Bacillus amyloliquefaciens intimate coupling is a potential means to accelerate its biotransformation. The initial steps of 2,4,6-TCP biodegradation involve mono-oxygenation reactions that have molecular oxygen and an intracellular electron carrier as cosubstrates. It was demonstrated that B. amyloliquefaciens has the 2,4,6-TCP monooxygenase gene tcpA which could encode 2,4,6-TCP monooxygenase (TCP-MO). TCP-MO would catalytically decompose 2,4,6-TCP into 2,6-DCHQ. We employed an internal loop photolytic biofilm reactor for 2,4,6-TCP degradation. Sequentially coupled photolysis and biodegradation experimental results suggested that 2,4,6-TCP removal rate in P + B (TCP(UV) + phenol) protocol was higher by 77 and 103 % when compared to B (TCP + phenol) and B (TCP-only) protocols respectively. The corresponding loss rate coefficient (k) values were 0.069, 0.039, 0.034 mg/L·min -1 respectively. This is because UV photolysis converted 2,4,6-TCP into its intermediates: 2,4-dichlorophenol (2,4-DCP), 4-monochlorophenol (4-MCP), phenol, 2,6-dichloro-p-hydroquinone (2,6-DCHQ), with all displaying less inhibition to bacterial action. In addition, phenol was the crucial UV-photolysis product from 2,4,6-TCP, its catabolic oxidation generating internal electron carriers that may accelerate the initial steps of 2,4,6-TCP biodegradation. Intimately coupled photolysis and biodegradation experimental results suggested that 2,4,6-TCP removal rate in P&B (TCP + phenol) protocol was higher by 166 and 681 % when compared to P&B (TCP-only) and P + B protocols respectively. The corresponding loss rate coefficient (k) values were 0.539, 0.203, 0.069 mg/L·min -1 respectively. It provided sufficient evidence to demonstrate that intimately coupled photolysis and biodegradation accelerated 2,4,6-TCP removal much faster than sequentially coupled photolysis and biodegradation. In addition, oxidation of phenol was the mechanism by which intimately coupled photolysis and biodegradation accelerated rapid 2,4,6-TCP removal producing electron equivalents that stimulated the initial mono-oxygenation reactions for 2,4,6-TCP biodegradation. It is important to note that 2,6-DCHQ (produced from UV-photolysis products or initial mono-oxygenation reactions) would be catalytically decomposed into 6-chlorohydroxyquinol (6-CHQ). Based on this, a tentative reaction mechanism for the photo-biodegradation 2,4,6-TCP was proposed.

  14. 48 CFR 246.270 - Safety of facilities, infrastructure, and equipment for military operations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ASSURANCE Contract Quality Requirements 246.270 Safety of facilities, infrastructure, and equipment for... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Safety of facilities, infrastructure, and equipment for military operations. 246.270 Section 246.270 Federal Acquisition Regulations...

  15. 48 CFR 552.246-70 - Source Inspection by Quality Approved Manufacturer.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Quality Approved Manufacturer. 552.246-70 Section 552.246-70 Federal Acquisition Regulations System... Provisions and Clauses 552.246-70 Source Inspection by Quality Approved Manufacturer. As prescribed in 546.302-70, insert the following clause: Source Inspection by Quality Approved Manufacturer (JUL 09) (a...

  16. 48 CFR 552.246-70 - Source Inspection by Quality Approved Manufacturer.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Quality Approved Manufacturer. 552.246-70 Section 552.246-70 Federal Acquisition Regulations System... Provisions and Clauses 552.246-70 Source Inspection by Quality Approved Manufacturer. As prescribed in 546.302-70, insert the following clause: Source Inspection by Quality Approved Manufacturer (JUL 09) (a...

  17. 40 CFR 246.201-3 - Recommended procedures: Glass, can, and mixed paper separation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., and mixed paper separation. 246.201-3 Section 246.201-3 Protection of Environment ENVIRONMENTAL... and Recommended Procedures § 246.201-3 Recommended procedures: Glass, can, and mixed paper separation. In areas where markets are available, it is recommended that glass, cans, and mixed paper be...

  18. 40 CFR 246.201-3 - Recommended procedures: Glass, can, and mixed paper separation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., and mixed paper separation. 246.201-3 Section 246.201-3 Protection of Environment ENVIRONMENTAL... and Recommended Procedures § 246.201-3 Recommended procedures: Glass, can, and mixed paper separation. In areas where markets are available, it is recommended that glass, cans, and mixed paper be...

  19. 7 CFR 246.16a - Infant formula cost containment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN State Agency Provisions § 246.16a Infant formula cost containment. (a) Who must use cost... 7 Agriculture 4 2011-01-01 2011-01-01 false Infant formula cost containment. 246.16a Section 246...

  20. 7 CFR 246.16a - Infant formula cost containment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN State Agency Provisions § 246.16a Infant formula cost containment. (a) Who must use cost... 7 Agriculture 4 2010-01-01 2010-01-01 false Infant formula cost containment. 246.16a Section 246...

  1. 9 CFR 354.246 - Exclusion of diseased persons.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Exclusion of diseased persons. 354.246 Section 354.246 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... Sanitary Conditions and Precautions Against Contamination of Products § 354.246 Exclusion of diseased...

  2. 48 CFR 52.246-4 - Inspection of Services-Fixed-Price.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-Fixed-Price. 52.246-4 Section 52.246-4 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-4 Inspection of Services—Fixed-Price. As prescribed in 46.304, insert the following clause: Inspection of Services—Fixed-Price (AUG 1996) (a) Definitions. Services, as used in this clause, includes...

  3. 42 CFR 24.6 - Pay and compensation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Pay and compensation. 24.6 Section 24.6 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES PERSONNEL SENIOR BIOMEDICAL RESEARCH SERVICE § 24.6 Pay and compensation. The SBRS is an ungraded system, with a single, flexible pay...

  4. 42 CFR 24.6 - Pay and compensation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Pay and compensation. 24.6 Section 24.6 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES PERSONNEL SENIOR BIOMEDICAL RESEARCH SERVICE § 24.6 Pay and compensation. The SBRS is an ungraded system, with a single, flexible pay...

  5. 42 CFR 24.6 - Pay and compensation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Pay and compensation. 24.6 Section 24.6 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES PERSONNEL SENIOR BIOMEDICAL RESEARCH SERVICE § 24.6 Pay and compensation. The SBRS is an ungraded system, with a single, flexible pay...

  6. 40 CFR 246.202-3 - Recommended procedures: Market study.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Market study. 246.202-3 Section 246.202-3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.202-3 Recommended procedures: Market study. An investigation of markets should be made by the...

  7. 40 CFR 246.201-4 - Recommended procedures: Market study.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Market study. 246.201-4 Section 246.201-4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.201-4 Recommended procedures: Market study. An investigation of markets should be made for each...

  8. 40 CFR 246.201-4 - Recommended procedures: Market study.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recommended procedures: Market study. 246.201-4 Section 246.201-4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.201-4 Recommended procedures: Market study. An investigation of markets should be made for each...

  9. 40 CFR 246.202-3 - Recommended procedures: Market study.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recommended procedures: Market study. 246.202-3 Section 246.202-3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.202-3 Recommended procedures: Market study. An investigation of markets should be made by the...

  10. 40 CFR 246.200-3 - Recommended procedures: Market study.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recommended procedures: Market study. 246.200-3 Section 246.200-3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.200-3 Recommended procedures: Market study. An investigation of markets should be made by the...

  11. 40 CFR 246.200-3 - Recommended procedures: Market study.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Market study. 246.200-3 Section 246.200-3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... § 246.200-3 Recommended procedures: Market study. An investigation of markets should be made by the...

  12. 12 CFR 19.246 - Petition for reinstatement.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Petition for reinstatement. 19.246 Section 19.246 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY RULES OF PRACTICE AND PROCEDURE Removal, Suspension, and Debarment of Accountants From Performing Audit Services § 19.246 Petition...

  13. 43 CFR 24.6 - Cooperative agreements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false Cooperative agreements. 24.6 Section 24.6... WILDLIFE POLICY: STATE-FEDERAL RELATIONSHIPS § 24.6 Cooperative agreements. (a) By reason of the... cooperative agreements for a variety of fish and wildlife programs on Federal lands. This practice shall be...

  14. 43 CFR 24.6 - Cooperative agreements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false Cooperative agreements. 24.6 Section 24.6... WILDLIFE POLICY: STATE-FEDERAL RELATIONSHIPS § 24.6 Cooperative agreements. (a) By reason of the... cooperative agreements for a variety of fish and wildlife programs on Federal lands. This practice shall be...

  15. 43 CFR 24.6 - Cooperative agreements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true Cooperative agreements. 24.6 Section 24.6... WILDLIFE POLICY: STATE-FEDERAL RELATIONSHIPS § 24.6 Cooperative agreements. (a) By reason of the... cooperative agreements for a variety of fish and wildlife programs on Federal lands. This practice shall be...

  16. 43 CFR 2.46 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Department or a bureau thereof, including, but not limited to, education, financial transactions, medical... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false Definitions. 2.46 Section 2.46 Public... ACT Privacy Act § 2.46 Definitions. (a) Act. As used in this subpart, “Act” means section 3 of the...

  17. 48 CFR 1852.246-73 - Human space flight item.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Human space flight item. 1852.246-73 Section 1852.246-73 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE... 1852.246-73 Human space flight item. As prescribed in 1845.370(b), insert the following clause: Human...

  18. 48 CFR 1852.246-73 - Human space flight item.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Human space flight item. 1852.246-73 Section 1852.246-73 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE... 1852.246-73 Human space flight item. As prescribed in 1845.370(b), insert the following clause: Human...

  19. 48 CFR 1852.246-73 - Human space flight item.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Human space flight item. 1852.246-73 Section 1852.246-73 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE... 1852.246-73 Human space flight item. As prescribed in 1845.370(b), insert the following clause: Human...

  20. 48 CFR 1852.246-73 - Human space flight item.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Human space flight item. 1852.246-73 Section 1852.246-73 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE... 1852.246-73 Human space flight item. As prescribed in 1845.370(b), insert the following clause: Human...

  1. 48 CFR 1852.246-73 - Human space flight item.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Human space flight item. 1852.246-73 Section 1852.246-73 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE... 1852.246-73 Human space flight item. As prescribed in 1845.370(b), insert the following clause: Human...

  2. 48 CFR 52.246-8 - Inspection of Research and Development-Cost-Reimbursement.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Development-Cost-Reimbursement. 52.246-8 Section 52.246-8 Federal Acquisition Regulations System FEDERAL... Provisions and Clauses 52.246-8 Inspection of Research and Development—Cost-Reimbursement. As prescribed in... (b) a cost-reimbursement contract is contemplated; unless use of the clause is impractical and the...

  3. 48 CFR 246.370 - Material inspection and receiving report.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Material inspection and receiving report. 246.370 Section 246.370 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 246.370 Material inspection and receiving report. (a)...

  4. 48 CFR 246.370 - Material inspection and receiving report.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Material inspection and receiving report. 246.370 Section 246.370 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 246.370 Material inspection and receiving report. (a)...

  5. 10 CFR 431.246 - Energy conservation standards and their effective dates.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 3 2014-01-01 2014-01-01 false Energy conservation standards and their effective dates. 431.246 Section 431.246 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ENERGY EFFICIENCY PROGRAM FOR CERTAIN COMMERCIAL AND INDUSTRIAL EQUIPMENT Unit Heaters Energy Conservation Standards § 431.246 Energy...

  6. 10 CFR 431.246 - Energy conservation standards and their effective dates.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false Energy conservation standards and their effective dates. 431.246 Section 431.246 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ENERGY EFFICIENCY PROGRAM FOR CERTAIN COMMERCIAL AND INDUSTRIAL EQUIPMENT Unit Heaters Energy Conservation Standards § 431.246 Energy...

  7. 10 CFR 431.246 - Energy conservation standards and their effective dates.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 3 2012-01-01 2012-01-01 false Energy conservation standards and their effective dates. 431.246 Section 431.246 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ENERGY EFFICIENCY PROGRAM FOR CERTAIN COMMERCIAL AND INDUSTRIAL EQUIPMENT Unit Heaters Energy Conservation Standards § 431.246 Energy...

  8. 10 CFR 431.246 - Energy conservation standards and their effective dates.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 3 2011-01-01 2011-01-01 false Energy conservation standards and their effective dates. 431.246 Section 431.246 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ENERGY EFFICIENCY PROGRAM FOR CERTAIN COMMERCIAL AND INDUSTRIAL EQUIPMENT Unit Heaters Energy Conservation Standards § 431.246 Energy...

  9. 25 CFR 900.246 - What does reassumption mean?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What does reassumption mean? 900.246 Section 900.246 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR, AND INDIAN HEALTH SERVICE, DEPARTMENT OF... Retrocession and Reassumption Procedures § 900.246 What does reassumption mean? Reassumption means rescission...

  10. 48 CFR 52.246-6 - Inspection-Time-and-Material and Labor-Hour.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...-Material and Labor-Hour. 52.246-6 Section 52.246-6 Federal Acquisition Regulations System FEDERAL... Provisions and Clauses 52.246-6 Inspection—Time-and-Material and Labor-Hour. As prescribed in 46.306, insert the following clause: Inspection—Time-and-Material and Labor-Hour (MAY 2001) (a) Definitions. As used...

  11. 48 CFR 52.246-6 - Inspection-Time-and-Material and Labor-Hour.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-Material and Labor-Hour. 52.246-6 Section 52.246-6 Federal Acquisition Regulations System FEDERAL... Provisions and Clauses 52.246-6 Inspection—Time-and-Material and Labor-Hour. As prescribed in 46.306, insert the following clause: Inspection—Time-and-Material and Labor-Hour (MAY 2001) (a) Definitions. As used...

  12. 40 CFR 246.201-3 - Recommended procedures: Glass, can, and mixed paper separation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... mixed paper separation. 246.201-3 Section 246.201-3 Protection of Environment ENVIRONMENTAL PROTECTION... Recommended Procedures § 246.201-3 Recommended procedures: Glass, can, and mixed paper separation. In areas where markets are available, it is recommended that glass, cans, and mixed paper be separated at the...

  13. 48 CFR 52.246-17 - Warranty of Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Noncomplex Nature. 52.246-17 Section 52.246-17 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-17 Warranty of Supplies of a Noncomplex Nature. As prescribed in 46.710(a)(1), insert a clause substantially as follows: Warranty of Supplies of a Noncomplex Nature (JUN 2003) (a) Definitions...

  14. 48 CFR 52.246-17 - Warranty of Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Noncomplex Nature. 52.246-17 Section 52.246-17 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-17 Warranty of Supplies of a Noncomplex Nature. As prescribed in 46.710(a)(1), insert a clause substantially as follows: Warranty of Supplies of a Noncomplex Nature (JUN 2003) (a) Definitions...

  15. 7 CFR 246.5 - Selection of local agencies.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 4 2011-01-01 2011-01-01 false Selection of local agencies. 246.5 Section 246.5... State and Local Agency Eligibility § 246.5 Selection of local agencies. (a) General. This section sets forth the procedures the State agency shall perform in the selection of local agencies and the expansion...

  16. 48 CFR 52.246-2 - Inspection of Supplies-Fixed-Price.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-Fixed-Price. 52.246-2 Section 52.246-2 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-2 Inspection of Supplies—Fixed-Price. As prescribed in 46.302, insert the following clause: Inspection of Supplies—Fixed-Price (AUG 1996) (a) Definition. Supplies, as used in this clause, includes but...

  17. 48 CFR 52.246-7 - Inspection of Research and Development-Fixed-Price.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Development-Fixed-Price. 52.246-7 Section 52.246-7 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-7 Inspection of Research and Development—Fixed-Price. As prescribed in 46.307(a), insert the following clause: Inspection of Research and Development—Fixed-Price (AUG 1996) (a) The Contractor...

  18. 48 CFR 552.246-72 - Final Inspection and Tests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Final Inspection and Tests. 552.246-72 Section 552.246-72 Federal Acquisition Regulations System GENERAL SERVICES ADMINISTRATION CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 552.246-72 Final Inspection and Tests. As prescribed...

  19. 48 CFR 52.246-17 - Warranty of Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Noncomplex Nature. 52.246-17 Section 52.246-17 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-17 Warranty of Supplies of a Noncomplex Nature. As prescribed in 46.710(a)(1), insert a clause substantially as follows: Warranty of Supplies of a Noncomplex Nature (JUN 2003) (a) Definitions...

  20. 48 CFR 52.246-17 - Warranty of Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Noncomplex Nature. 52.246-17 Section 52.246-17 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-17 Warranty of Supplies of a Noncomplex Nature. As prescribed in 46.710(a)(1), insert a clause substantially as follows: Warranty of Supplies of a Noncomplex Nature (JUN 2003) (a) Definitions...

  1. 48 CFR 52.246-17 - Warranty of Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Noncomplex Nature. 52.246-17 Section 52.246-17 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-17 Warranty of Supplies of a Noncomplex Nature. As prescribed in 46.710(a)(1), insert a clause substantially as follows: Warranty of Supplies of a Noncomplex Nature (JUN 2003) (a) Definitions...

  2. Effects of Postchallenge Administration of ST-246 on Dissemination of IHD-J-Luc Vaccinia Virus in Normal Mice and in Immune-Deficient Mice Reconstituted with T Cells

    PubMed Central

    Shotwell, Elisabeth; Scott, John; Cruz, Stephanie; King, Lisa R.; Manischewitz, Jody; Diaz, Claudia G.; Jordan, Robert A.; Grosenbach, Douglas W.; Golding, Hana

    2013-01-01

    Whole-body bioimaging was used to study dissemination of vaccinia virus (VACV) in normal and in immune deficient (nu−/nu−) mice protected from lethality by postchallenge administration of ST-246. Total fluxes were recorded in the liver, spleen, lungs, and nasal cavities of live mice after intranasal infection with a recombinant IHD-J-Luc VACV expressing luciferase. Areas under the flux curve were calculated for individual mice to assess viral loads. Treatment for 2 to 5 days of normal BALB/c mice with ST-246 at 100 mg/kg starting 24 h postchallenge conferred 100% protection and reduced viral loads in four organs compared to control mice. Mice also survived after 5 days of treatment with ST-246 at 30 mg/kg, and yet the viral loads and poxes were higher in these mice compared to 100-mg/kg treatment group. Nude mice were not protected by ST-246 alone or by 10 million adoptively transferred T cells. In contrast, nude mice that received T cells and 7-day treatment with ST-246 survived infection and exhibited reduced viral loads compared to nonreconstituted and ST-246-treated mice after ST-246 was stopped. Similar protection of nude mice was achieved using adoptively transferred 1.0 and 0.1 million, but not 0.01 million, purified T cells or CD4+ or CD8+ T cells in conjunction with ST-246 treatment. These data suggest that ST-246 protects immunocompetent mice from lethality and reduces viral dissemination in internal organs and poxvirus lesions. Furthermore, immune-deficient animals with partial T cell reconstitution can control virus replication after a course of ST-246 and survive lethal vaccinia virus challenge. PMID:23468500

  3. 42 CFR 137.246 - How does a Self-Governance Tribe retrocede a PSFA?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false How does a Self-Governance Tribe retrocede a PSFA? 137.246 Section 137.246 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES....246 How does a Self-Governance Tribe retrocede a PSFA? The Self-Governance Tribe submits a written...

  4. 42 CFR 137.246 - How does a Self-Governance Tribe retrocede a PSFA?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false How does a Self-Governance Tribe retrocede a PSFA? 137.246 Section 137.246 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES....246 How does a Self-Governance Tribe retrocede a PSFA? The Self-Governance Tribe submits a written...

  5. 42 CFR 137.246 - How does a Self-Governance Tribe retrocede a PSFA?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false How does a Self-Governance Tribe retrocede a PSFA? 137.246 Section 137.246 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES....246 How does a Self-Governance Tribe retrocede a PSFA? The Self-Governance Tribe submits a written...

  6. 42 CFR 137.246 - How does a Self-Governance Tribe retrocede a PSFA?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false How does a Self-Governance Tribe retrocede a PSFA? 137.246 Section 137.246 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES....246 How does a Self-Governance Tribe retrocede a PSFA? The Self-Governance Tribe submits a written...

  7. 48 CFR 52.246-18 - Warranty of Supplies of a Complex Nature.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Complex Nature. 52.246-18 Section 52.246-18 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-18 Warranty of Supplies of a Complex Nature. As prescribed in 46.710(b)(1), insert a clause substantially as follows: Warranty of Supplies of a Complex Nature (MAY 2001) (a) Definitions. As used in this...

  8. 48 CFR 552.246-77 - Additional Contract Warranty Provisions for Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Warranty Provisions for Supplies of a Noncomplex Nature. 552.246-77 Section 552.246-77 Federal Acquisition... a Noncomplex Nature. As prescribed in 546.710(a), insert the following clause in solicitations and contracts that include FAR 52.246-17, Warranty of Supplies of a Noncomplex Nature. Additional Contract...

  9. 48 CFR 52.246-18 - Warranty of Supplies of a Complex Nature.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Complex Nature. 52.246-18 Section 52.246-18 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-18 Warranty of Supplies of a Complex Nature. As prescribed in 46.710(b)(1), insert a clause substantially as follows: Warranty of Supplies of a Complex Nature (MAY 2001) (a) Definitions. As used in this...

  10. 48 CFR 52.246-18 - Warranty of Supplies of a Complex Nature.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Complex Nature. 52.246-18 Section 52.246-18 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-18 Warranty of Supplies of a Complex Nature. As prescribed in 46.710(b)(1), insert a clause substantially as follows: Warranty of Supplies of a Complex Nature (MAY 2001) (a) Definitions. As used in this...

  11. 48 CFR 552.246-77 - Additional Contract Warranty Provisions for Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Warranty Provisions for Supplies of a Noncomplex Nature. 552.246-77 Section 552.246-77 Federal Acquisition... a Noncomplex Nature. As prescribed in 546.710(a), insert the following clause in solicitations and contracts that include FAR 52.246-17, Warranty of Supplies of a Noncomplex Nature. Additional Contract...

  12. 48 CFR 552.246-77 - Additional Contract Warranty Provisions for Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Warranty Provisions for Supplies of a Noncomplex Nature. 552.246-77 Section 552.246-77 Federal Acquisition... a Noncomplex Nature. As prescribed in 546.710(a), insert the following clause in solicitations and contracts that include FAR 52.246-17, Warranty of Supplies of a Noncomplex Nature. Additional Contract...

  13. 48 CFR 552.246-77 - Additional Contract Warranty Provisions for Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Warranty Provisions for Supplies of a Noncomplex Nature. 552.246-77 Section 552.246-77 Federal Acquisition... a Noncomplex Nature. As prescribed in 546.710(a), insert the following clause in solicitations and contracts that include FAR 52.246-17, Warranty of Supplies of a Noncomplex Nature. Additional Contract...

  14. 48 CFR 552.246-77 - Additional Contract Warranty Provisions for Supplies of a Noncomplex Nature.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Warranty Provisions for Supplies of a Noncomplex Nature. 552.246-77 Section 552.246-77 Federal Acquisition... a Noncomplex Nature. As prescribed in 546.710(a), insert the following clause in solicitations and contracts that include FAR 52.246-17, Warranty of Supplies of a Noncomplex Nature. Additional Contract...

  15. 48 CFR 52.246-18 - Warranty of Supplies of a Complex Nature.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Complex Nature. 52.246-18 Section 52.246-18 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-18 Warranty of Supplies of a Complex Nature. As prescribed in 46.710(b)(1), insert a clause substantially as follows: Warranty of Supplies of a Complex Nature (MAY 2001) (a) Definitions. As used in this...

  16. 48 CFR 52.246-18 - Warranty of Supplies of a Complex Nature.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Complex Nature. 52.246-18 Section 52.246-18 Federal Acquisition Regulations System FEDERAL ACQUISITION... Clauses 52.246-18 Warranty of Supplies of a Complex Nature. As prescribed in 46.710(b)(1), insert a clause substantially as follows: Warranty of Supplies of a Complex Nature (MAY 2001) (a) Definitions. As used in this...

  17. Preparation of 1,3,5-triamino-2,4,6-trinitrobenzene from 3,5-dichloranisole

    DOEpatents

    Ott, Donald G.; Benziger, Theodore M.

    1990-01-01

    Preparation of 1,3,5-triamino-2,4,6-trinitrobenzene (TATB) from 3,5-dichloroanisole. Nitration of 3,5-dichloroanisole under relatively mild conditions gave 3,5-dichloro-2,4,6-trinitroanisole in high yield and purity. Ammonolysis of this latter compound gave the desired TATB. Another route to TATB was through the treatment of the 3,5-dichloro-2,4,6-trinitroanisole with thionyl chloride and dimethylformamide to yield 1,3,5-trichloro-2,4,6-trinitrobenzene. Ammonolysis of this product produced TATB.

  18. Preparation of 1,3,5-triamino-2,4,6-trinitrobenzene from 3,5-dichloroanisole

    DOEpatents

    Ott, Donald G.; Benziger, Theodore M.

    1991-01-01

    Preparation of 1,3,5-triamino-2,4,6-trinitrobenzene (TATB) from 3,5-dichloroanisole. Nitration of 3,5-dichloroanisole under relatively mild conditions gave 3,5-dichloro-2,4,6-trinitroanisole in high yield and purity. Ammonolysis of this latter compound gave the desired TATB. Another route to TATB was through the treatment of the 3,5-dichloro-2,4,6-trinitroanisole with thionyl chloride and dimethylformamide to yield 1,3,5-trichloro-2,4,6-trinitrobenzene. Ammonolysis of this product produced TATB.

  19. Preparation of 1,3,5-triamino-2,4,6-trinitrobenzene from 3,5-dichloroanisole

    DOEpatents

    Ott, D.G.; Benziger, T.M.

    1991-03-05

    Preparation of 1,3,5-triamino-2,4,6-trinitrobenzene (TATB) from 3,5-dichloroanisole is described. Nitration of 3,5-dichloroanisole under relatively mild conditions gave 3,5-dichloro-2,4,6-trinitroanisole in high yield and purity. Ammonolysis of this latter compound gave the desired TATB. Another route to TATB was through the treatment of the 3,5-dichloro-2,4,6-trinitroanisole with thionyl chloride and dimethylformamide to yield 1,3,5-trichloro-2,4,6-trinitrobenzene. Ammonolysis of this product produced TATB. 8 figures.

  20. 48 CFR 246.704 - Authority for use of warranties.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Warranties 246.704 Authority for use... quality assurance provisions that reference higher-level contract quality requirements (see 246.202-4); or...

  1. 33 CFR 148.246 - When is a document considered filed and where should I file it?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... filed and where should I file it? 148.246 Section 148.246 Navigation and Navigable Waters COAST GUARD... Formal Hearings § 148.246 When is a document considered filed and where should I file it? (a) If a document to be filed is submitted by mail, it is considered filed on the date it is postmarked. If a...

  2. Anti-leukaemic effects induced by APR-246 are dependent on induction of oxidative stress and the NFE2L2/HMOX1 axis that can be targeted by PI3K and mTOR inhibitors in acute myeloid leukaemia cells.

    PubMed

    Ali, Dina; Mohammad, Dara K; Mujahed, Huthayfa; Jonson-Videsäter, Kerstin; Nore, Beston; Paul, Christer; Lehmann, Sören

    2016-07-01

    The small molecule APR-246 (PRIMA-1(MET) ) is a novel drug that restores the activity of mutated and unfolded TP53 protein. However, the mechanisms of action and potential off-target effects are not fully understood. Gene expression profiling in TP53 mutant KMB3 acute myeloid leukaemia (AML) cells showed that genes which protected cells from oxidative stress to be the most up-regulated. APR-246 exposure also induced reactive oxygen species (ROS) formation and depleted glutathione in AML cells. The genes most up-regulated by APR-246, confirmed by quantitative real time polymerase chain reaction, were heme oxygenase-1 (HMOX1, also termed HO-1), SLC7A11 and RIT1. Up-regulation of HMOX1, a key regulator of cellular response to ROS, was independent of TP53 mutational status. NFE2L2 (also termed Nrf2), a master regulator of HMOX1 expression, showed transcriptional up-regulation and nuclear translocation by APR-246. Down-regulation of NFE2L2 by siRNA in AML cells significantly increased the antitumoural effects of APR-246. The PI3K inhibitor wortmannin and the mTOR inhibitor rapamycin inhibited APR-246-induced nuclear translocation of NFE2L2 and counteracted the protective cellular responses to APR-246, resulting in synergistic cell killing together with APR-246. In conclusion, ROS induction is important for antileukaemic activities of APR-246 and inhibiting the protective response of the Nrf-2/HMOX1 axis using PI3K inhibitors, enhances the antileukaemic effects. © 2016 John Wiley & Sons Ltd.

  3. 40 CFR 246.200 - High-grade paper recovery.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false High-grade paper recovery. 246.200 Section 246.200 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE... paper recovery. ...

  4. 40 CFR 246.200 - High-grade paper recovery.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false High-grade paper recovery. 246.200 Section 246.200 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE... paper recovery. ...

  5. Approximating the r-Process on Earth with Thermonuclear Explosions. Lessons Learned and Unanswered Questions

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Becker, Stephen Allan

    2016-01-28

    During the astrophysical r-process, multiple neutron captures occur so rapidly on target nuclei that their daughter nuclei generally do not have time to undergo radioactive decay before another neutron is captured. The r-process can be approximately simulated on Earth in certain types of thermonuclear explosions through an analogous process of rapid neutron captures known as the "prompt capture" process. Between 1952 and 1969, 23 nuclear tests were fielded by the US which were involved (at least partially) with the "prompt capture" process. Of these tests, 15 were at least partially successful. Some of these tests were conducted under the Plowsharemore » Peaceful Nuclear Explosion Program as scientific research experiments. It is now known that the USSR conducted similar nuclear tests during 1966 to 1979. The elements einsteinium and fermium were first discovered by this process. The most successful tests achieved 19 successive neutron captures on the initial target nuclei. A review of the US program, target nuclei used, heavy element yields, scientific achievements of the program, and how some of the results have been used by the astrophysical community is given. Finally, some unanswered questions concerning very neutron-rich nuclei that could potentially have been answered with additional nuclear experiments is presented.« less

  6. 48 CFR 246.601 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 246.601 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Material Inspection and Receiving Reports 246.601 General. See Appendix F, Material Inspection and Receiving Report, for procedures and instructions for the use...

  7. 48 CFR 246.601 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 246.601 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Material Inspection and Receiving Reports 246.601 General. See Appendix F, Material Inspection and Receiving Report, for procedures and instructions for the use...

  8. 48 CFR 246.601 - General.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 246.601 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Material Inspection and Receiving Reports 246.601 General. See Appendix F, Material Inspection and Receiving Report, for procedures and instructions for the use...

  9. 48 CFR 246.601 - General.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 246.601 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Material Inspection and Receiving Reports 246.601 General. See Appendix F, Material Inspection and Receiving Report, for procedures and instructions for the use...

  10. 48 CFR 246.601 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 246.601 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Material Inspection and Receiving Reports 246.601 General. See Appendix F, Material Inspection and Receiving Report, for procedures and instructions for the use...

  11. 48 CFR 246.471 - Authorizing shipment of supplies.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... supplies. 246.471 Section 246.471 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance... contract administration office signs or stamps the shipping papers that accompany Government source...

  12. 48 CFR 246.471 - Authorizing shipment of supplies.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... supplies. 246.471 Section 246.471 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance... contract administration office signs or stamps the shipping papers that accompany Government source...

  13. 48 CFR 246.471 - Authorizing shipment of supplies.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... supplies. 246.471 Section 246.471 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance... contract administration office signs or stamps the shipping papers that accompany Government source...

  14. 48 CFR 246.471 - Authorizing shipment of supplies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... supplies. 246.471 Section 246.471 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance... contract administration office signs or stamps the shipping papers that accompany Government source...

  15. 48 CFR 246.471 - Authorizing shipment of supplies.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... supplies. 246.471 Section 246.471 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance... contract administration office signs or stamps the shipping papers that accompany Government source...

  16. 40 CFR 246.100 - Scope.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Scope. 246.100 Section 246.100 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR... source separation of residential, commercial, and institutional solid wastes. Explicitly excluded are...

  17. 40 CFR 246.100 - Scope.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Scope. 246.100 Section 246.100 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR... source separation of residential, commercial, and institutional solid wastes. Explicitly excluded are...

  18. 40 CFR 246.100 - Scope.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Scope. 246.100 Section 246.100 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR... source separation of residential, commercial, and institutional solid wastes. Explicitly excluded are...

  19. 40 CFR 246.100 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Scope. 246.100 Section 246.100 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR... source separation of residential, commercial, and institutional solid wastes. Explicitly excluded are...

  20. 40 CFR 246.100 - Scope.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Scope. 246.100 Section 246.100 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES SOURCE SEPARATION FOR... source separation of residential, commercial, and institutional solid wastes. Explicitly excluded are...

  1. 48 CFR 246.406 - Foreign governments.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Foreign governments. 246..., DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.406 Foreign governments. (1) Quality assurance among North Atlantic Treaty Organization (NATO) countries. (i...

  2. 48 CFR 246.406 - Foreign governments.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Foreign governments. 246..., DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.406 Foreign governments. (1) Quality assurance among North Atlantic Treaty Organization (NATO) countries. (i...

  3. 48 CFR 246.406 - Foreign governments.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Foreign governments. 246..., DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.406 Foreign governments. (1) Quality assurance among North Atlantic Treaty Organization (NATO) countries. (i...

  4. 48 CFR 246.406 - Foreign governments.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Foreign governments. 246..., DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.406 Foreign governments. (1) Quality assurance among North Atlantic Treaty Organization (NATO) countries. (i...

  5. 48 CFR 246.406 - Foreign governments.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Foreign governments. 246..., DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.406 Foreign governments. (1) Quality assurance among North Atlantic Treaty Organization (NATO) countries. (i...

  6. Brominated dioxins/furans and hydroxylated polybrominated diphenyl ethers: Occurrences in commercial 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE) and 2,4,6-tribromophenol, and formation during synthesis of BTBPE.

    PubMed

    Ren, Man; Zeng, Hao; Peng, Ping-An; Li, Hui-Ru; Tang, Cai-Ming; Hu, Jian-Fang

    2017-07-01

    Polybrominated dibenzo-p-dioxins (PBDDs) and hydroxylated polybrominated diphenyl ethers (OH-PBDEs) can be formed from bromophenols (BPs) by thermal degradation, biosynthesis or phototransformation. However, it is unknown whether PBDDs and OH-PBDEs can be formed during the chemical production processes that utilize BPs as raw materials. 2,4,6-tribromophenol (2,4,6-TBP) is an important raw material for the synthesis of 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE), a novel brominated flame retardant. In this study, PBDDs, polybrominated dibenzofurans (PBDFs) and OH-PBDEs have been identified and quantified in commercially available BTBPE and 2,4,6-TBP. Furthermore, their formation as unintentional by-products during the laboratory synthesis of BTBPE from 2,4,6-TBP and 1,2-dibromoethane in the presence of sodium carbonate has also been investigated. 2,3,7,8-substituted PBDDs and PBDFs (2,3,7,8-PBDD/Fs) were undetectable in commercial samples of BTBPE and present in low levels (nanogram per gram) in 2,4,6-TBP. Two tetrabrominated dibenzo-p-dioxins (TeBDDs), namely 1,3,6,8- and 1,3,7,9-TeBDD, and three hydroxylated pentabrominated diphenyl ethers (OH-pentaBDEs), namely 4'-OH-BDE121, 2'-OH-BDE121, and 6'-OH-BDE100, were identified or tentatively identified, and quantitatively estimated to be at concentrations in the range of undetectable to several thousands of nanograms per gram in commercial BTBPE and 2,4,6-TBP. TeBDDs and OH-pentaBDEs were formed as by-products from 2,4,6-TBP during BTBPE synthesis. Further studies need to be conducted in order to determine whether PBDD/Fs and OH-PBDEs are also formed during the industrial synthesis of other chemical compounds that utilize BPs as raw materials or intermediates. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. 8 CFR 246.5 - Hearing.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 8 Aliens and Nationality 1 2012-01-01 2012-01-01 false Hearing. 246.5 Section 246.5 Aliens and... Hearing. (a) Service counsel. The Government shall be represented at the hearing by a Service counsel who... attend or remain in attendance at the hearing. ...

  8. 7 CFR 246.11 - Nutrition education.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 4 2012-01-01 2012-01-01 false Nutrition education. 246.11 Section 246.11 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN...

  9. 7 CFR 246.11 - Nutrition education.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 4 2013-01-01 2013-01-01 false Nutrition education. 246.11 Section 246.11 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN...

  10. 7 CFR 246.11 - Nutrition education.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 4 2011-01-01 2011-01-01 false Nutrition education. 246.11 Section 246.11 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN...

  11. 7 CFR 246.11 - Nutrition education.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Nutrition education. 246.11 Section 246.11 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN...

  12. 7 CFR 246.11 - Nutrition education.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 4 2014-01-01 2014-01-01 false Nutrition education. 246.11 Section 246.11 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN...

  13. 24 CFR 246.20 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Applicability. 246.20 Section 246.20 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  14. 24 CFR 246.20 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Applicability. 246.20 Section 246.20 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  15. 24 CFR 246.31 - Procedures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Procedures. 246.31 Section 246.31 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  16. 24 CFR 246.20 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Applicability. 246.20 Section 246.20 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  17. 24 CFR 246.31 - Procedures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Procedures. 246.31 Section 246.31 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  18. 24 CFR 246.4 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Applicability. 246.4 Section 246.4 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  19. 24 CFR 246.6 - Initiation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Initiation. 246.6 Section 246.6 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  20. 24 CFR 246.4 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Applicability. 246.4 Section 246.4 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  1. 24 CFR 246.4 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Applicability. 246.4 Section 246.4 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  2. 24 CFR 246.6 - Initiation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Initiation. 246.6 Section 246.6 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  3. 24 CFR 246.20 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Applicability. 246.20 Section 246.20 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  4. 24 CFR 246.22 - Procedures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Procedures. 246.22 Section 246.22 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  5. 24 CFR 246.31 - Procedures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Procedures. 246.31 Section 246.31 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  6. 24 CFR 246.22 - Procedures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Procedures. 246.22 Section 246.22 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  7. 24 CFR 246.22 - Procedures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Procedures. 246.22 Section 246.22 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  8. 24 CFR 246.6 - Initiation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Initiation. 246.6 Section 246.6 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  9. 24 CFR 246.20 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Applicability. 246.20 Section 246.20 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  10. 24 CFR 246.31 - Procedures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Procedures. 246.31 Section 246.31 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  11. 24 CFR 246.22 - Procedures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Procedures. 246.22 Section 246.22 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  12. 24 CFR 246.6 - Initiation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Initiation. 246.6 Section 246.6 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  13. 24 CFR 246.22 - Procedures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Procedures. 246.22 Section 246.22 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  14. 24 CFR 246.4 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Applicability. 246.4 Section 246.4 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  15. 24 CFR 246.4 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Applicability. 246.4 Section 246.4 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  16. 24 CFR 246.31 - Procedures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Procedures. 246.31 Section 246.31 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  17. 24 CFR 246.6 - Initiation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Initiation. 246.6 Section 246.6 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  18. 48 CFR 1652.246-70 - FEHB Inspection.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false FEHB Inspection. 1652.246-70 Section 1652.246-70 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT FEDERAL EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION CLAUSES AND FORMS CONTRACT CLAUSES Texts of FEHBP Clauses...

  19. 48 CFR 252.246-7000 - Material inspection and receiving report.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... receiving report. 252.246-7000 Section 252.246-7000 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... extent required by Appendix F, Material Inspection and Receiving Report, of the Defense FAR Supplement...

  20. 48 CFR 252.246-7000 - Material inspection and receiving report.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... receiving report. 252.246-7000 Section 252.246-7000 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... extent required by Appendix F, Material Inspection and Receiving Report, of the Defense FAR Supplement...

  1. 48 CFR 252.246-7000 - Material inspection and receiving report.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... receiving report. 252.246-7000 Section 252.246-7000 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... extent required by Appendix F, Material Inspection and Receiving Report, of the Defense FAR Supplement...

  2. 48 CFR 252.246-7000 - Material inspection and receiving report.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... receiving report. 252.246-7000 Section 252.246-7000 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... extent required by Appendix F, Material Inspection and Receiving Report, of the Defense FAR Supplement...

  3. 48 CFR 252.246-7000 - Material inspection and receiving report.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... receiving report. 252.246-7000 Section 252.246-7000 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... extent required by Appendix F, Material Inspection and Receiving Report, of the Defense FAR Supplement...

  4. 50 CFR 665.246 - Gear identification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Gear identification. 665.246 Section 665.246 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC... Gear identification. In Permit Area 1, the vessel's official number must be marked legibly on all traps...

  5. 24 CFR 246.30 - Rental charges.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Rental charges. 246.30 Section 246.30 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  6. 24 CFR 246.9 - Request for preemption.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Request for preemption. 246.9 Section 246.9 Housing and Urban Development Regulations Relating to Housing and Urban Development... URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES...

  7. 24 CFR 246.10 - HUD procedures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false HUD procedures. 246.10 Section 246.10 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  8. 24 CFR 246.9 - Request for preemption.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Request for preemption. 246.9 Section 246.9 Housing and Urban Development Regulations Relating to Housing and Urban Development... URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES...

  9. 24 CFR 246.10 - HUD procedures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false HUD procedures. 246.10 Section 246.10 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  10. 24 CFR 246.5 - Rental charges.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Rental charges. 246.5 Section 246.5 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  11. 24 CFR 246.9 - Request for preemption.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Request for preemption. 246.9 Section 246.9 Housing and Urban Development Regulations Relating to Housing and Urban Development... URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES...

  12. 24 CFR 246.5 - Rental charges.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Rental charges. 246.5 Section 246.5 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  13. 24 CFR 246.10 - HUD procedures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false HUD procedures. 246.10 Section 246.10 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  14. 24 CFR 246.5 - Rental charges.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Rental charges. 246.5 Section 246.5 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  15. 24 CFR 246.5 - Rental charges.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Rental charges. 246.5 Section 246.5 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  16. 24 CFR 246.10 - HUD procedures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false HUD procedures. 246.10 Section 246.10 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  17. 24 CFR 246.5 - Rental charges.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Rental charges. 246.5 Section 246.5 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

  18. 24 CFR 246.30 - Rental charges.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Rental charges. 246.30 Section 246.30 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  19. 24 CFR 246.10 - HUD procedures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false HUD procedures. 246.10 Section 246.10 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  20. 24 CFR 246.30 - Rental charges.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Rental charges. 246.30 Section 246.30 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  1. 24 CFR 246.9 - Request for preemption.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Request for preemption. 246.9 Section 246.9 Housing and Urban Development Regulations Relating to Housing and Urban Development... URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES...

  2. 24 CFR 246.30 - Rental charges.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Rental charges. 246.30 Section 246.30 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  3. 24 CFR 246.9 - Request for preemption.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Request for preemption. 246.9 Section 246.9 Housing and Urban Development Regulations Relating to Housing and Urban Development... URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES...

  4. 24 CFR 246.30 - Rental charges.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Rental charges. 246.30 Section 246.30 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  5. 48 CFR 252.246-7006 - Warranty Tracking of Serialized Items.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Warranty Tracking of Serialized Items. 252.246-7006 Section 252.246-7006 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT...

  6. 48 CFR 252.246-7006 - Warranty Tracking of Serialized Items.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Warranty Tracking of Serialized Items. 252.246-7006 Section 252.246-7006 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT...

  7. 48 CFR 252.246-7006 - Warranty Tracking of Serialized Items.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Warranty Tracking of Serialized Items. 252.246-7006 Section 252.246-7006 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT...

  8. 48 CFR 252.246-7006 - Warranty Tracking of Serialized Items.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Warranty Tracking of Serialized Items. 252.246-7006 Section 252.246-7006 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT...

  9. 48 CFR 852.246-72 - Frozen processed foods.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

  10. 48 CFR 852.246-72 - Frozen processed foods.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

  11. 48 CFR 852.246-72 - Frozen processed foods.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

  12. 48 CFR 852.246-72 - Frozen processed foods.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

  13. 48 CFR 852.246-72 - Frozen processed foods.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

  14. 21 CFR 520.246 - Butorphanol tartrate tablets.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ....246 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS ORAL DOSAGE FORM NEW ANIMAL DRUGS § 520.246 Butorphanol... associated with inflammatory conditions of the upper respiratory tract. (3) Limitations. For oral use in dogs...

  15. 21 CFR 520.246 - Butorphanol tartrate tablets.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ....246 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS ORAL DOSAGE FORM NEW ANIMAL DRUGS § 520.246 Butorphanol... associated with inflammatory conditions of the upper respiratory tract. (3) Limitations. For oral use in dogs...

  16. 21 CFR 520.246 - Butorphanol tartrate tablets.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ....246 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS ORAL DOSAGE FORM NEW ANIMAL DRUGS § 520.246 Butorphanol... associated with inflammatory conditions of the upper respiratory tract. (3) Limitations. For oral use in dogs...

  17. 24 CFR 246.11 - Notification of action on preemption request.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Notification of action on preemption request. 246.11 Section 246.11 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER...

  18. 24 CFR 246.7 - Notice to tenants.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Notice to tenants. 246.7 Section 246.7 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  19. 24 CFR 246.1 - Scope and effect of regulations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Scope and effect of regulations. 246.1 Section 246.1 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  20. 24 CFR 246.11 - Notification of action on preemption request.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Notification of action on preemption request. 246.11 Section 246.11 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER...

  1. 24 CFR 246.7 - Notice to tenants.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Notice to tenants. 246.7 Section 246.7 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  2. 24 CFR 246.11 - Notification of action on preemption request.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Notification of action on preemption request. 246.11 Section 246.11 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER...

  3. 24 CFR 246.1 - Scope and effect of regulations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Scope and effect of regulations. 246.1 Section 246.1 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  4. 24 CFR 246.7 - Notice to tenants.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Notice to tenants. 246.7 Section 246.7 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  5. 24 CFR 246.11 - Notification of action on preemption request.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Notification of action on preemption request. 246.11 Section 246.11 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER...

  6. 24 CFR 246.1 - Scope and effect of regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Scope and effect of regulations. 246.1 Section 246.1 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  7. 24 CFR 246.1 - Scope and effect of regulations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Scope and effect of regulations. 246.1 Section 246.1 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  8. 24 CFR 246.11 - Notification of action on preemption request.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Notification of action on preemption request. 246.11 Section 246.11 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER...

  9. 24 CFR 246.7 - Notice to tenants.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Notice to tenants. 246.7 Section 246.7 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  10. 24 CFR 246.7 - Notice to tenants.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOCAL RENT... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Notice to tenants. 246.7 Section 246.7 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  11. 24 CFR 246.1 - Scope and effect of regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Scope and effect of regulations. 246.1 Section 246.1 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  12. 32 CFR 246.6 - Procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Procedures. 246.6 Section 246.6 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND...) The Stars and Stripes and the S&S business operations shall conform to applicable regulations and laws...

  13. 32 CFR 246.1 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Purpose. 246.1 Section 246.1 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND... Stars and Stripes, including the resale of commercial publications necessary to support the overall S&S...

  14. 32 CFR 246.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 2 2011-07-01 2011-07-01 false Purpose. 246.1 Section 246.1 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND... Stars and Stripes, including the resale of commercial publications necessary to support the overall S&S...

  15. 32 CFR 246.6 - Procedures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Procedures. 246.6 Section 246.6 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND...) The Stars and Stripes and the S&S business operations shall conform to applicable regulations and laws...

  16. 32 CFR 246.1 - Purpose.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Purpose. 246.1 Section 246.1 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND... Stars and Stripes, including the resale of commercial publications necessary to support the overall S&S...

  17. 32 CFR 246.6 - Procedures.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Procedures. 246.6 Section 246.6 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND...) The Stars and Stripes and the S&S business operations shall conform to applicable regulations and laws...

  18. 32 CFR 246.1 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Purpose. 246.1 Section 246.1 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS STARS AND... Stars and Stripes, including the resale of commercial publications necessary to support the overall S&S...

  19. 48 CFR 246.470-2 - Quality evaluation data.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 246.470-2 Quality evaluation data. The contract administration office shall establish a system for the collection, evaluation, and use of the types of quality evaluation data specified in PGI 246.470-2. [71 FR... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Quality evaluation data...

  20. 48 CFR 246.470-2 - Quality evaluation data.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 246.470-2 Quality evaluation data. The contract administration office shall establish a system for the collection, evaluation, and use of the types of quality evaluation data specified in PGI 246.470-2. [71 FR... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Quality evaluation data...

  1. 48 CFR 246.470-2 - Quality evaluation data.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 246.470-2 Quality evaluation data. The contract administration office shall establish a system for the collection, evaluation, and use of the types of quality evaluation data specified in PGI 246.470-2. [71 FR... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Quality evaluation data...

  2. 48 CFR 246.470-2 - Quality evaluation data.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 246.470-2 Quality evaluation data. The contract administration office shall establish a system for the collection, evaluation, and use of the types of quality evaluation data specified in PGI 246.470-2. [71 FR... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Quality evaluation data...

  3. 28 CFR 2.46 - Execution of warrant and service of summons.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 1 2012-07-01 2012-07-01 false Execution of warrant and service of summons. 2.46 Section 2.46 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND... procedural rights under the Commission's regulations and the possible actions which may be taken by the...

  4. 28 CFR 2.46 - Execution of warrant and service of summons.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Execution of warrant and service of summons. 2.46 Section 2.46 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND... procedural rights under the Commission's regulations and the possible actions which may be taken by the...

  5. 28 CFR 2.46 - Execution of warrant and service of summons.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Execution of warrant and service of summons. 2.46 Section 2.46 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND... procedural rights under the Commission's regulations and the possible actions which may be taken by the...

  6. 28 CFR 2.46 - Execution of warrant and service of summons.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 1 2014-07-01 2014-07-01 false Execution of warrant and service of summons. 2.46 Section 2.46 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND... procedural rights under the Commission's regulations and the possible actions which may be taken by the...

  7. 28 CFR 2.46 - Execution of warrant and service of summons.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 1 2013-07-01 2013-07-01 false Execution of warrant and service of summons. 2.46 Section 2.46 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND... procedural rights under the Commission's regulations and the possible actions which may be taken by the...

  8. 48 CFR 252.246-7004 - Safety of Facilities, Infrastructure, and Equipment for Military Operations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...: SAFETY OF FACILITIES, INFRASTRUCTURE, AND EQUIPMENT FOR MILITARY OPERATIONS (OCT 2010) (a) Definition... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Safety of Facilities, Infrastructure, and Equipment for Military Operations. 252.246-7004 Section 252.246-7004 Federal Acquisition...

  9. SEDIMENT-MEDIATED REDUCTION OF 2,4,6-TRINITROTOLUENE AND FATE OF THE RESULTING AROMATIC (POLY)AMINES

    EPA Science Inventory

    2,4,6-Trinitrotoluene is a major surface and subsurface contaminant found at numerous munitions production and storage facilities. The reductive transformation of 2,4,6-trinitrotoluene (TNT) to aromatic (poly)amines and the consequent fate of these products were studied in anaer...

  10. 10 CFR 72.246 - Issuance of amendment to a certificate of compliance.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Issuance of amendment to a certificate of compliance. 72.246 Section 72.246 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN...

  11. 24 CFR 246.12 - Preemption of prospective term of lease.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Preemption of prospective term of lease. 246.12 Section 246.12 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  12. 24 CFR 246.12 - Preemption of prospective term of lease.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Preemption of prospective term of lease. 246.12 Section 246.12 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  13. 24 CFR 246.12 - Preemption of prospective term of lease.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Preemption of prospective term of lease. 246.12 Section 246.12 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  14. 24 CFR 246.12 - Preemption of prospective term of lease.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Preemption of prospective term of lease. 246.12 Section 246.12 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  15. 24 CFR 246.12 - Preemption of prospective term of lease.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Preemption of prospective term of lease. 246.12 Section 246.12 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF...

  16. 12 CFR 24.6 - Examples of qualifying public welfare investments.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Examples of qualifying public welfare investments. 24.6 Section 24.6 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY COMMUNITY AND ECONOMIC DEVELOPMENT ENTITIES, COMMUNITY DEVELOPMENT PROJECTS, AND OTHER PUBLIC WELFARE...

  17. 12 CFR 303.246 - Conversion with diminution of capital.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 4 2011-01-01 2011-01-01 false Conversion with diminution of capital. 303.246... FILING PROCEDURES Other Filings § 303.246 Conversion with diminution of capital. (a) Scope. This section... such conversion. (b) Where to file. Applicants shall submit a letter application to the appropriate...

  18. 12 CFR 303.246 - Conversion with diminution of capital.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Conversion with diminution of capital. 303.246... FILING PROCEDURES Other Filings § 303.246 Conversion with diminution of capital. (a) Scope. This section... such conversion. (b) Where to file. Applicants shall submit a letter application to the appropriate...

  19. 21 CFR 520.246 - Butorphanol tartrate tablets.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Butorphanol tartrate tablets. 520.246 Section 520.246 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... tartrate tablets. (a) Specifications. Each tablet contains 1, 5, or 10 milligrams of butorphanol base...

  20. ST-246 inhibits in vivo poxvirus dissemination, virus shedding, and systemic disease manifestation.

    PubMed

    Berhanu, Aklile; King, David S; Mosier, Stacie; Jordan, Robert; Jones, Kevin F; Hruby, Dennis E; Grosenbach, Douglas W

    2009-12-01

    Orthopoxvirus infections, such as smallpox, can lead to severe systemic disease and result in considerable morbidity and mortality in immunologically naïve individuals. Treatment with ST-246, a small-molecule inhibitor of virus egress, has been shown to provide protection against severe disease and death induced by several members of the poxvirus family, including vaccinia, variola, and monkeypox viruses. Here, we show that ST-246 treatment not only results in the significant inhibition of vaccinia virus dissemination from the site of inoculation to distal organs, such as the spleen and liver, but also reduces the viral load in organs targeted by the dissemination. In mice intranasally infected with vaccinia virus, virus shedding from the nasal and lung mucosa was significantly lower (approximately 22- and 528-fold, respectively) upon ST-246 treatment. Consequently, virus dissemination from the nasal site of replication to the lung also was dramatically reduced, as evidenced by a 179-fold difference in virus levels in nasal versus bronchoalveolar lavage. Furthermore, in ACAM2000-immunized mice, vaccination site swabs showed that ST-246 treatment results in a major (approximately 3,900-fold by day 21) reduction in virus detected at the outside surfaces of lesions. Taken together, these data suggest that ST-246 would play a dual protective role if used during a smallpox bioterrorist attack. First, ST-246 would provide therapeutic benefit by reducing the disease burden and lethality in infected individuals. Second, by reducing virus shedding from those prophylactically immunized with a smallpox vaccine or harboring variola virus infection, ST-246 could reduce the risk of virus transmission to susceptible contacts.

  1. 32 CFR Appendix C to Part 246 - Personnel Policies and Procedures

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Personnel Policies and Procedures C Appendix C... (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS Pt. 246, App. C Appendix C to Part 246—Personnel Policies and Procedures A. General Nonappropriated Fund (NAF) Employment...

  2. 7 CFR 246.5 - Selection of local agencies.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... consider how much of the current need is being met at each priority level. The selection criteria cited in... 7 Agriculture 4 2010-01-01 2010-01-01 false Selection of local agencies. 246.5 Section 246.5 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF...

  3. 48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

  4. 48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

  5. 48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

  6. 48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

  7. 48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

  8. In vitro susceptibility to ST-246 and Cidofovir corroborates the phylogenetic separation of Brazilian Vaccinia virus into two clades.

    PubMed

    Pires, Mariana A; Rodrigues, Nathália F S; de Oliveira, Danilo B; de Assis, Felipe L; Costa, Galileu B; Kroon, Erna G; Mota, Bruno E F

    2018-04-01

    The Orthopoxvirus (OPV) genus of the Poxviridae family contains several human pathogens, including Vaccinia virus (VACV), which have been implicating in outbreaks of a zoonotic disease called Bovine Vaccinia in Brazil. So far, no approved treatment exists for OPV infections, but ST-246 and Cidofovir (CDV) are now in clinical development. Therefore, the objective of this work was to evaluate the susceptibility of five strains of Brazilian VACV (Br-VACV) to ST-246 and Cidofovir. The susceptibility of these strains to both drugs was evaluated by plaque reduction assay, extracellular virus's quantification in the presence of ST-246 and one-step growth curve in cells treated with CDV. Besides that, the ORFs F13L and E9L were sequenced for searching of polymorphisms associated with drug resistance. The effective concentration of 50% (EC 50 ) from both drugs varies significantly for different strains (from 0.0054 to 0.051 μM for ST-246 and from 27.14 to 61.23 μM for CDV). ST-246 strongly inhibits the production of extracellular virus for all isolates in concentrations as low as 0.1 μM and it was observed a relevant decrease of progeny production for all Br-VACV after CDV treatment. Sequencing of the F13L and E9L ORFs showed that Br-VACV do not present the polymorphism(s) associated with resistance to ST-246 and CDV. Taken together, our results showed that ST-246 and CDV are effective against diverse, wild VACV strains and that the susceptibility of Br-VACV to these drugs mirrored the phylogenetic split of these isolates into two groups. Thus, both ST-246 and CDV are of great interest as compounds to treat individuals during Bovine Vaccinia outbreaks in Brazil. Copyright © 2018 Elsevier B.V. All rights reserved.

  9. Biodegradation of 2,4,6-trichlorophenol in a packed-bed biofilm reactor equipped with an internal net draft tube riser for aeration and liquid circulation.

    PubMed

    Jesús, A Gómez-De; Romano-Baez, F J; Leyva-Amezcua, L; Juárez-Ramírez, C; Ruiz-Ordaz, N; Galíndez-Mayer, J

    2009-01-30

    For the aerobic biodegradation of the fungicide and defoliant 2,4,6-trichlorophenol (2,4,6-TCP), a bench-scale packed-bed bioreactor equipped with a net draft tube riser for liquid circulation and oxygenation (PB-ALR) was constructed. To obtain a high packed-bed volume relative to the whole bioreactor volume, a high A(D)/A(R) ratio was used. Reactor's downcomer was packed with a porous support of volcanic stone fragments. PB-ALR hydrodynamics and oxygen mass transfer behavior was evaluated and compared to the observed behavior of the unpacked reactor operating as an internal airlift reactor (ALR). Overall gas holdup values epsilon(G), and zonal oxygen mass transfer coefficients determined at various airflow rates in the PB-ALR, were higher than those obtained with the ALR. When comparing mixing time values obtained in both cases, a slight increment in mixing time was observed when reactor was operated as a PB-ALR. By using a mixed microbial community, the biofilm reactor was used to evaluate the aerobic biodegradation of 2,4,6-TCP. Three bacterial strains identified as Burkholderia sp., Burkholderia kururiensis and Stenotrophomonas sp. constituted the microbial consortium able to cometabolically degrade the 2,4,6-TCP, using phenol as primary substrate. This consortium removed 100% of phenol and near 99% of 2,4,6-TCP. Mineralization and dehalogenation of 2,4,6-TCP was evidenced by high COD removal efficiencies ( approximately 95%), and by the stoichiometric release of chloride ions from the halogenated compound ( approximately 80%). Finally, it was observed that the microbial consortium was also capable to metabolize 2,4,6-TCP without phenol as primary substrate, with high removal efficiencies (near 100% for 2,4,6-TCP, 92% for COD and 88% for chloride ions).

  10. 24 CFR 246.8 - Materials to be submitted to HUD in support of preemption request.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Materials to be submitted to HUD in support of preemption request. 246.8 Section 246.8 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING...

  11. 24 CFR 246.8 - Materials to be submitted to HUD in support of preemption request.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Materials to be submitted to HUD in support of preemption request. 246.8 Section 246.8 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING...

  12. 24 CFR 246.8 - Materials to be submitted to HUD in support of preemption request.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Materials to be submitted to HUD in support of preemption request. 246.8 Section 246.8 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING...

  13. 48 CFR 52.246-19 - Warranty of Systems and Equipment under Performance Specifications or Design Criteria.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 2 2014-10-01 2014-10-01 false Warranty of Systems and Equipment under Performance Specifications or Design Criteria. 52.246-19 Section 52.246-19 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of...

  14. 32 CFR Appendix E to Part 246 - Stars and Stripes (S&S) Board of Directors

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Stars and Stripes (S&S) Board of Directors E... DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS Pt. 246, App. E Appendix E to Part 246—Stars and Stripes (S&S) Board of Directors A. Organization and Management...

  15. 32 CFR Appendix E to Part 246 - Stars and Stripes (S&S) Board of Directors

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Stars and Stripes (S&S) Board of Directors E... DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS Pt. 246, App. E Appendix E to Part 246—Stars and Stripes (S&S) Board of Directors A. Organization and Management...

  16. 32 CFR Appendix E to Part 246 - Stars and Stripes (S&S) Board of Directors

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Stars and Stripes (S&S) Board of Directors E... DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS Pt. 246, App. E Appendix E to Part 246—Stars and Stripes (S&S) Board of Directors A. Organization and Management...

  17. Bioactive Formylated Flavonoids from Eugenia rigida: Isolation, Synthesis, and X-ray Crystallography.

    PubMed

    Zaki, Mohamed A; Nanayakkara, N P Dhammika; Hetta, Mona H; Jacob, Melissa R; Khan, Shabana I; Mohammed, Rabab; Ibrahim, Mohamed A; Samoylenko, Volodymyr; Coleman, Christina; Fronczek, Frank R; Ferreira, Daneel; Muhammad, Ilias

    2016-09-23

    Two new flavonoids, rac-6-formyl-5,7-dihydroxyflavanone (1) and 2',6'-dihydroxy-4'-methoxy-3'-methylchalcone (2), together with five known derivatives, rac-8-formyl-5,7-dihydroxyflavanone (3), 4',6'-dihydroxy-2'-methoxy-3'-methyldihydrochalcone (4), rac-7-hydroxy-5-methoxy-6-methylflavanone (5), 3'-formyl-2',4',6'-trihydroxy-5'-methyldihydrochalcone (6), and 3'-formyl-2',4',6'-trihydroxydihydrochalcone (7), were isolated from the leaves of Eugenia rigida. The individual (S)- and (R)-enantiomers of 1 and 3, together with the corresponding formylated flavones 8 (6-formyl-5,7-dihydroxyflavone) and 9 (8-formyl-5,7-dihydroxyflavone), as well as 2',4',6'-trihydroxychalcone (10), 3'-formyl-2',4',6'-trihydroxychalcone (11), and the corresponding 3'-formyl-2',4',6'-trihydroxydihydrochalcone (7) and 2',4',6'-trihydroxydihydrochalcone (12), were synthesized. The structures of the isolated and synthetic compounds were established via NMR, HRESIMS, and electronic circular dichroism data. In addition, the structures of 3, 5, and 8 were confirmed by single-crystal X-ray diffraction crystallography. The isolated and synthetic flavonoids were evaluated for their antimicrobial and cytotoxic activities against a panel of microorganisms and solid tumor cell lines.

  18. Ultrasound-assisted emulsification microextraction for determination of 2,4,6-trichloroanisole in wine samples by gas chromatography tandem mass spectrometry.

    PubMed

    Fontana, Ariel R; Patil, Sangram H; Banerjee, Kaushik; Altamirano, Jorgelina C

    2010-04-28

    A fast and effective microextraction technique is proposed for preconcentration of 2,4,6-trichloroanisole (2,4,6-TCA) from wine samples prior gas chromatography tandem mass spectrometric (GC-MS/MS) analysis. The proposed technique is based on ultrasonication (US) for favoring the emulsification phenomenon during the extraction stage. Several variables influencing the relative response of the target analyte were studied and optimized. Under optimal experimental conditions, 2,4,6-TCA was quantitatively extracted achieving enhancement factors (EF) > or = 400 and limits of detection (LODs) 0.6-0.7 ng L(-1) with relative standard deviations (RSDs) < or = 11.3%, when 10 ng L(-1) 2,4,6-TCA standard-wine sample blend was analyzed. The calibration graphs for white and red wine were linear within the range of 5-1000 ng L(-1), and estimation coefficients (r(2)) were > or = 0.9995. Validation of the methodology was carried out by standard addition method at two concentrations (10 and 50 ng L(-1)) achieving recoveries >80% indicating satisfactory robustness of the method. The methodology was successfully applied for determination of 2,4,6-TCA in different wine samples.

  19. Gas-liquid reactive crystallization kinetics of 2,4,6-triamino-1,3,5-trinitrobenzene in the semi-batch procedure

    NASA Astrophysics Data System (ADS)

    Liu, Ruqin; Huang, Ming; Yao, Xiaolu; Chen, Shuang; Wang, Shucun; Suo, Zhirong

    2018-06-01

    2,4,6-Triamino-1,3,5-trinitrobenzene is the attractive insensitive high energetic material used extensively in the military and civil fields. Combined with the double-films theory, the global gas-liquid chemical reaction kinetics of 2,4,6-triamino-1,3,5-trinitrobenzene was developed by means of the infinitesimal material balance calculation. The raw material concentration and reactive temperature effects on the crystallization of 2,4,6-triamino-1,3,5-trinitrobenzene were investigated by the batch experiments. The reactive crystallization kinetics associated ammonia feeding rate of 2,4,6-triamino-1,3,5-trinitrobenzene, including nucleation as well as crystal growth, was systematically investigated in the heterogonous semi-batch procedure. The nucleation and crystal growth kinetic exponents were estimated by the linear least-squares method. The crystallization kinetic results indicated that nucleation rate strongly increased but liner growth rate decreased with the increasing of ammonia feeding rate. In terms of manufacturing coarse 2,4,6-triamino-1,3,5-trinitrobenzene, it was found that a slow ammonia feeding rate and a low raw material concentration were feasible under the present experimental conditions.

  20. Simultaneous removal of 2,4,6-tribromophenol from water and bromate ion minimization by ozonation.

    PubMed

    Gounden, Asogan N; Singh, Sooboo; Jonnalagadda, Sreekantha B

    2018-06-02

    The study investigates the degradation of 2,4,6-tribromophenol (2,4,6-TBP) and the influence of solution pH, alkalinity, H 2 O 2 and O 3 dosage. Debromination efficiency of 2,4,6-TBP was the highest in basic water (pH = 10.61). The extent of TOC removal compared favourably with the amount of substrate converted, suggesting favourable mineralization of oxygenated by-products (OBPs). Ozonation in basic water favoured the formation of toxicBrO 3 - , while in acidic water (pH = 2.27) BrO 3 - yield was lowest. In acidic water the presence of CO 3 2- showed negligible effect on conversion, TOC and  BrO 3 - yield compared to ozonation alone. In basic water both 2,4,6-TBP conversion and TOC removal decreased with an increase in CO 3 2- , hence minimizing BrO 3 - formation. The O 3 /H 2 O 2 process showed an improvement in the debromination efficiency and TOC data revealed that total mineralization of OBP's was achieved. However, only 10% H 2 O 2 was able to effectively decrease BrO 3 - formation. Increasing the ozone concentration from 20 to 100 ppm enhanced the conversion of 2,4,6-TBP and TOC removal. At low ozone concentrations poor mineralization of OBP's occurred, while complete mineralization was achieved at higher ozone dose. The reaction pathways for ozone degradation of 2,4,6-TBP in acidic and basic waters is proposed. Copyright © 2018 Elsevier B.V. All rights reserved.

  1. Evaluating nurse prescribers' education and continuing professional development for independent prescribing practice: findings from a national survey in England.

    PubMed

    Latter, Sue; Maben, Jill; Myall, Michelle; Young, Amanda

    2007-10-01

    The number of nurses able to independently prescribe medicines in England has risen steadily in recent years. To evaluate the adequacy of nurses' educational preparation for independent prescribing and to describe nurses' experiences of their continuing professional development as prescribers in practice. Postal questionnaire survey. Random sample of 246 nurses registered as nurse independent prescribers with the Nursing and Midwifery Council. The majority of nurses considered that the initial taught course element of their education programme met their needs, either to some extent (61% 151/246), or completely (22% 54/246). Most nurses (77% 190/246) received the specified 12 days support from their supervising medical practitioner and most were satisfied and positive about this experience. Nearly all of the nurses (>95%) reported that they were able to maintain a range of specified prescribing competencies in practice. Two thirds (62% 152/246) of the sample reported that they were receiving support/supervision for prescribing. Ninety five per cent (233/246) of the sample also reported that they engaged in self-directed informal continuing professional development, but only half of the sample had experience of formally provided professional development opportunities. Approximately half (52% 127/246) of the sample identified needs for continuing professional development. This first national survey of the education and professional development experiences of nurse independent prescribers in England provides evidence which highlights areas in which national policy is working well, and also points up issues which may need addressing as the roll out of nurse prescribing continues. The study also highlights characteristics and issues that health care policy makers and nurse educationalists internationally may wish to consider in developing and refining their own nurse prescriber education programmes.

  2. APR-246/PRIMA-1Met Inhibits and Reverses Squamous Metaplasia in Human Conjunctival Epithelium.

    PubMed

    Li, Jing; Li, Cheng; Wang, Guoliang; Liu, Zhen; Chen, Pei; Yang, Qichen; Dong, Nuo; Wu, Huping; Liu, Zuguo; Li, Wei

    2016-02-01

    Squamous metaplasia is a common pathologic condition in ocular surface diseases for which there is no therapeutic medication in clinic. In this study, we investigated the effect of a small molecule, APR-246/PRIMA-1(Met), on squamous metaplasia in human conjunctival epithelium. Human conjunctival explants were cultured for up to 12 days under airlifting conditions. Epithelial cell differentiation and proliferation were assessed by Cytokeratin 10 (K10), K14, K19, Pax6, MUC5AC, and p63 immunostaining patterns. β-catenin and TCF-4 immunofluorescent staining and real-time PCR characterized Wnt signaling pathway involvement. Pterygium clinical samples were cultured under airlifting conditions with or without APR-246 for 4 days. p63, K10, β-catenin, and TCF-4 expression in pterygial epithelium was determined by immunofluorescent staining and real-time PCR. Airlift conjunctival explants resulted in increased stratification and intrastromal epithelial invagination. Such pathology was accompanied by increases in K10, K14, and p63 expression, whereas K19 and Pax6 levels declined when compared to those in freshly isolated tissue. On the other hand, APR-246 reversed all of these declines in K10, K14, and p63 expression. Furthermore, K19 and Pax6 increased along with rises in goblet cell density. These effects of APR-246 were accompanied by near restoration of normal conjunctival epithelial histology. APR-246 also reversed squamous metaplasia in pterygial epithelium that had developed after 4 days in ex vivo culture. Reductions in squamous metaplasia induced by APR-246 suggest it may provide a novel therapeutic approach in different squamous metaplasia-associated ocular surface diseases.

  3. Synthesis of Heteroaryl Sulfonamides from Organozinc Reagents and 2,4,6-Trichlorophenyl Chlorosulfate

    PubMed Central

    2016-01-01

    A method for the preparation of aryl and heteroaryl sulfonamides using 2,4,6-trichlorophenyl chlorosulfate (TCPC) is described. The reaction of 2-pyridylzinc reagents with TCPC resulted in 2,4,6-trichlorophenyl (TCP) pyridine-2-sulfonates, and the parent pyridine-2-sulfonate was shown to react with amines. Less electron-rich aryl- and heteroarylzinc reagents reacted with TCPC to afford sulfonyl chlorides that were converted in situ to sulfonamides. PMID:26065317

  4. 40 CFR 721.7280 - 1,3-Propanediamine, N,N′-1,2-ethanediylbis-, polymer with 2,4,6-trichloro-1,3,5-triazine...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...-ethanediylbis-, polymer with 2,4,6-trichloro-1,3,5-triazine, reaction products with N-butyl-2,2,6,6-tetramethyl...-, polymer with 2,4,6-trichloro-1,3,5-triazine, reaction products with N-butyl-2,2,6,6-tetramethyl-4..., reaction products with N-butyl-2,2,6,6-tetramethyl-4-piperidinamine (PMN P-89-632) is subject to reporting...

  5. 40 CFR 721.7280 - 1,3-Propanediamine, N,N′-1,2-ethanediylbis-, polymer with 2,4,6-trichloro-1,3,5-triazine...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...-ethanediylbis-, polymer with 2,4,6-trichloro-1,3,5-triazine, reaction products with N-butyl-2,2,6,6-tetramethyl...-, polymer with 2,4,6-trichloro-1,3,5-triazine, reaction products with N-butyl-2,2,6,6-tetramethyl-4..., reaction products with N-butyl-2,2,6,6-tetramethyl-4-piperidinamine (PMN P-89-632) is subject to reporting...

  6. Toward Serotonin Fluorescent False Neurotransmitters: Development of Fluorescent Dual Serotonin and Vesicular Monoamine Transporter Substrates for Visualizing Serotonin Neurons.

    PubMed

    Henke, Adam; Kovalyova, Yekaterina; Dunn, Matthew; Dreier, Dominik; Gubernator, Niko G; Dincheva, Iva; Hwu, Christopher; Šebej, Peter; Ansorge, Mark S; Sulzer, David; Sames, Dalibor

    2018-05-16

    Ongoing efforts in our laboratories focus on design of optical reporters known as fluorescent false neurotransmitters (FFNs) that enable the visualization of uptake into, packaging within, and release from individual monoaminergic neurons and presynaptic sites in the brain. Here, we introduce the molecular probe FFN246 as an expansion of the FFN platform to the serotonergic system. Combining the acridone fluorophore with the ethylamine recognition element of serotonin, we identified FFN54 and FFN246 as substrates for both the serotonin transporter and the vesicular monoamine transporter 2 (VMAT2). A systematic structure-activity study revealed the basic structural chemotype of aminoalkyl acridones required for serotonin transporter (SERT) activity and enabled lowering the background labeling of these probes while maintaining SERT activity, which proved essential for obtaining sufficient signal in the brain tissue (FFN246). We demonstrate the utility of FFN246 for direct examination of SERT activity and SERT inhibitors in 96-well cell culture assays, as well as specific labeling of serotonergic neurons of the dorsal raphe nucleus in the living tissue of acute mouse brain slices. While we found only minor FFN246 accumulation in serotonergic axons in murine brain tissue, FFN246 effectively traces serotonin uptake and packaging in the soma of serotonergic neurons with improved photophysical properties and loading parameters compared to known serotonin-based fluorescent tracers.

  7. Photochemical transformation of five novel brominated flame retardants: Kinetics and photoproducts.

    PubMed

    Zhang, Ya-Nan; Chen, Jingwen; Xie, Qing; Li, Yingjie; Zhou, Chengzhi

    2016-05-01

    Many novel brominated flame retardants (NBFRs) are used as substitutes of polybrominated diphenyl ethers (PBDEs) in recent years. However, little is known about their phototransformation behavior, which may influence the environmental fate of these chemicals. In this study, photochemical behavior of five NBFRs, allyl-2,4,6-tribromophenyl ether (ATE), 2-bromoallyl-2,4,6-tribromophenyl ether (BATE), 2,3-dibromopropyl-2,4,6-tribromophenyl ether (DPTE), 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE), and 2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine (TTBP-TAZ) was investigated. Results show all the five NBFRs can undergo photochemical transformation under simulated sunlight irradiation. Quantum yields (Φ) of the five NBFRs varied from 0.012 of TTBP-TAZ in hexane to 0.091 of BTBPE in methanol. Half-lives (t1/2) relevant with solar irradiation of these NBFRs were estimated using the determined Φ, and the values are 1.5-12.0 d in summer and 17.1-165.0 d in winter. Debrominated and ether bond cleavage products were identified in the phototransformation of DPTE and BTBPE. Debromination on the phenyl is a main phototransformation pathway for DPTE, and both debromination and ether bond cleavage are main phototransformation pathways for BTBPE. This study is helpful to better understand the phototransformation behavior of the NBFRs. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Degradation of Chlorophenols by Alcaligenes eutrophus JMP134(pJP4) in Bleached Kraft Mill Effluent

    PubMed Central

    Valenzuela, J.; Bumann, U.; Cespedes, R.; Padilla, L.; Gonzalez, B.

    1997-01-01

    The ability of Alcaligenes eutrophus JMP134(pJP4) to degrade 2,4-dichlorophenoxyacetic acid, 2,4,6-trichlorophenol, and other chlorophenols in a bleached kraft mill effluent was studied. The efficiency of degradation and the survival of strain JMP134 and indigenous microorganisms in short-term batch or long-term semicontinuous incubations performed in microcosms were assessed. After 6 days of incubation, 2,4-dichlorophenoxyacetate (400 ppm) or 2,4,6-trichlorophenol (40 to 100 ppm) were extensively degraded (70 to 100%). In short-term batch incubations, indigenous microorganisms were unable to degrade such of compounds. Degradation of 2,4,6-trichlorophenol by strain JMP134 was significantly lower at 200 to 400 ppm of compound. This strain was also able to degrade 2,4-dichlorophenoxyacetate, 2,4,6-trichlorophenol, 4-chlorophenol, and 2,4,5-trichlorophenol when bleached Kraft mill effluent was amended with mixtures of these compounds. On the other hand, the chlorophenol concentration and the indigenous microorganisms inhibited the growth and survival of the strain in short-term incubations. In long-term (>1-month) incubations, strain JMP134 was unable to maintain a large, stable population, although extensive 2,4,6-trichlorophenol degradation was still observed. The latter is probably due to acclimation of the indigenous microorganisms to degrade 2,4,6-trichlorophenol. Acclimation was observed only in long-term, semicontinuous microcosms. PMID:16535488

  9. Growth and characterization of a new nonlinear optical organic crystal: 2,4,6-Trimethylacetanilide

    NASA Astrophysics Data System (ADS)

    Upadhyaya, V.; Prabhu, Sharada G.

    2015-09-01

    A new nonlinear optical organic material, 2,4,6-trimethylacetanilide (246TMAA), also known as N-[2,4,6- trimethylphenyl]acetamide, has been synthesized and grown as a single crystal by the slow evaporation technique by organic solvents. The grown crystals have been characterized by morphology study. The crystals are prismatic. Surface examination shows granular dendritic pattern in optical micrograph. The Scanning Electron Micrograph shows the layered growth of the crystal. The Differential Scanning Calorimeter plot shows no phase change until melting point (219°C). The density of the crystals is 1.1g/cc and the crystals are soft. The crystals are transparent in the visible region and in the ultra-violet region till 280 nm. 246TMAA crystallizes with 2 molecules in a monoclinic unit cell in the noncentrosymmetric point group m, space group Pn. Refractive indices of this optically biaxial crystal along the three crystallophysical axes have been measured at 633 nm. The optical second harmonic generation efficiency of the crystal at 1064 nm is about half that of the urea crystal, measured by powder method using Nd:YAG laser. The results show that the 246TMAA crystal can efficiently be used for up-conversion of infrared radiation into visible green light. The powder X-ray diffraction spectrum of the crystal has been obtained.

  10. Biosynthesis of poly-beta-hydroxyalkanoates by Sphingopyxis chilensis S37 and Wautersia sp. PZK cultured in cellulose pulp mill effluents containing 2,4,6-trichlorophenol.

    PubMed

    Tobella, Lorena M; Bunster, Marta; Pooley, Amalia; Becerra, José; Godoy, Felix; Martínez, Miguel A

    2005-09-01

    Poly-beta-hydroxyalkanoates (PHA) polymer is synthesized by different bacterial species. There has been considerable interest in the development and production of biodegradable polymers; however, the high cost of PHA production has restricted its applications. Kraft cellulose industry effluents containing 2,4,6-trichlorophenol (10 or 20 microg ml(-1)) were used by the bacteria Sphingopyxis chilensis S37 and Wautersia sp. PZK to synthesize PHA. In this condition, S. chilensis S37 was able to grow and degrade 2,4,6-trichlorophenol (ca. 60%) and 80% of these cells accumulated PHA. Wautersia PZK completely degraded 2,4,6-TCP and more than 90% of the cells accumulated PHA in 72 h. The PHA detection was performed by flow cytometry and polyester composition was characterized by gas chromatography-mass spectroscopy (GC-MS), indicating that these polymers are made by 3-hydroxybutyric acid and 3-hydroxyhexadecanoic acid for S37 and PZK strains, respectively. Results demonstrated that strains' growth and PHA production and composition are not modified in cellulose effluents with or without 2,4,6-TCP (10-20 microg ml(-1)). Therefore, our results indicate that S. chilensis S37 and Wautersia sp. PZK are able to degrade a toxic compound such as a 2,4,6-TCP and simultaneously produce a valuable biopolymer using low-value substrates.

  11. 48 CFR 246.408-70 - Subsistence.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.408-70... quality in food, to perform quality assurance for subsistence contract items. The designation may— (1...

  12. Transformation of the Ionic X-Ray Contrast Agent Diatrizoate and Related Triiodinated Benzoates by Trametes versicolor

    PubMed Central

    Rode, Ulrike; Müller, Rudolf

    1998-01-01

    Iodinated X-ray contrast agents are considered to be nondegradable by microorganisms. The decomposition of the ionic X-ray contrast agents Diatrizoate (3,5-di(acetamido)-2,4,6-triiodobenzoic acid) and Iodipamide (3,3′-adipoyl-diimino-di(2,4,6-triiodobenzoic acid) and related triiodinated benzoates (Acetrizoate [3-acetylamino-2,4,6-triiodobenzoic acid] and Aminotrizoate [3-amino-2,4,6-triiodobenzoic acid]) by Trametes versicolor has been investigated. The fungus was able to transform all tested triiodinated benzoates cometabolically. During transformation of these compounds, iodide was released, but deiodination was not complete. T. versicolor liberated traces of 14CO2 from uniformly ring-14C-labeled Diatrizoate (3,5-di(acetamido)-2,4,6-triiodobenzoate). Various extracellular metabolites were detected during transformation of the different substances. In the transformation of Diatrizoate, the three main metabolites were identified as 3,5-di(acetamido)-2,6-diiodobenzoic acid, 3,5-di(acetamido)-2,4-diiodobenzoic acid, and 3,5-di(acetamido)-2-iodobenzoic acid, suggesting reductive deiodinations in steps as initial transformation steps. PMID:9687487

  13. Characterization of Coconut cadang-cadang viroid variants from oil palm affected by orange spotting disease in Malaysia.

    PubMed

    Wu, Y H; Cheong, L C; Meon, S; Lau, W H; Kong, L L; Joseph, H; Vadamalai, G

    2013-06-01

    A 246-nt variant of Coconut cadang-cadang viroid (CCCVd) has been identified and described from oil palms with orange spotting symptoms in Malaysia. Compared with the 246-nt form of CCCVd from coconut, the oil palm variant substituted C(31)→U in the pathogenicity domain and G(70)→C in the central conserved domain. This is the first sequence reported for a 246-nt variant of CCCVd in oil palms expressing orange spotting symptoms.

  14. 7 CFR 246.18 - Administrative review of State agency actions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) The State agency's determination to include or exclude an infant formula manufacturer, wholesaler... WOMEN, INFANTS AND CHILDREN State Agency Provisions § 246.18 Administrative review of State agency...

  15. 48 CFR 246.370 - Material inspection and receiving report.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 246.370... to issue orders. [56 FR 36460, July 31, 1991, as amended at 64 FR 2598, Jan. 15, 1999] ...

  16. 40 CFR 52.246 - Control of dry cleaning solvent vapor losses.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.246 Control of dry... cleaning establishment that uses solvents containing 4 percent or more by volume of any reactive organic...

  17. 48 CFR 246.710 - Solicitation provision and contract clauses.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of Construction (Germany), instead of the clause at FAR 52.246-21, Warranty of Construction, in... performance will be in Germany. (3)(i) In addition to 252.211-7003, Item Unique Identification and Valuation...

  18. 40 CFR 180.430 - Fenoxaprop-ethyl; tolerances for residues.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... combined residues of the herbicide fenoxaprop-ethyl [(±)-ethyl 2-[4-[(6-chloro-2-benzoxazolyl)oxy]phenoxy... herbicide fenoxaprop-ethyl, [(±)-ethyl 2-[4-[(6-chloro-2-benzoxazolyl)oxy]phenoxy]propanoic acid], and its...

  19. 48 CFR 246.102 - Policy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE General 246.102 Policy. Departments and agencies shall also— (1) Develop and manage a systematic, cost-effective Government contract quality assurance program to ensure that contract performance conforms to specified requirements. Apply Government quality assurance...

  20. 48 CFR 246.710 - Solicitation provision and contract clauses.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... contemplated. (4) Use the clause at 252.246-7002, Warranty of Construction (Germany), instead of the clause at...-price contract will be awarded and contract performance will be in Germany. (5)(i) In addition to 252...

  1. 48 CFR 246.710 - Solicitation provision and contract clauses.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... contemplated. (4) Use the clause at 252.246-7002, Warranty of Construction (Germany), instead of the clause at...-price contract will be awarded and contract performance will be in Germany. (5)(i) In addition to 252...

  2. 48 CFR 246.710 - Solicitation provision and contract clauses.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... contemplated. (4) Use the clause at 252.246-7002, Warranty of Construction (Germany), instead of the clause at...-price contract will be awarded and contract performance will be in Germany. (5)(i) In addition to 252...

  3. 48 CFR 246.202 - Types of contract quality requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 246.202 Types of contract quality requirements. ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Types of contract quality...

  4. Thioredoxin Activates MKK4-NFκB Pathway in a Redox-dependent Manner to Control Manganese Superoxide Dismutase Gene Expression in Endothelial Cells*

    PubMed Central

    Kundumani-Sridharan, Venkatesh; Subramani, Jaganathan; Das, Kumuda C.

    2015-01-01

    The mitogen-activated protein kinase kinase 4 (MKK4) is activated via phosphorylation of Ser-257 and Thr-261 by upstream MAP3Ks and activates JNK and p38 MAPKs in response to cellular stress. We show that thioredoxin (Trx), a cellular redox protein, activates MKK4 via Cys-246 and Cys-266 residues as mutation of these residues renders MKK4 insensitive to phosphorylation by MAP3Ks, TNFα, or Trx. MKK4 is activated in vitro by reduced Trx but not oxidized Trx in the absence of an upstream kinase, suggesting that autophosphorylation of this protein occurs due to reduction of Cys-246 and Cys-266 by Trx. Additionally, mutation of Cys-246 and Cys-266 resulted in loss of kinase activity suggesting that the redox state of Cys-246 and Cys-266 is a critical determinant of MKK4 activation. Trx induces manganese superoxide dismutase (MnSOD) gene transcription by activating MKK4 via redox control of Cys-246 and Cys-266, as mutation of these residues abrogates MKK4 activation and MnSOD expression. We further show that MKK4 activates NFκB for its binding to the MnSOD promoter, which leads to AP-1 dissociation followed by MnSOD transcription. Taken together, our studies show that the redox status of Cys-246 and Cys-266 in MKK4 controls its activities independent of MAP3K, demonstrating integration of the endothelial redox environment to MAPK signaling. PMID:26028649

  5. 48 CFR 246.401 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.401 General. The requirement for a quality assurance surveillance plan shall be addressed and documented in the contract file... services, the contracting officer should prepare a quality assurance surveillance plan to facilitate...

  6. 32 CFR 246.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.1 Purpose. This part: (a) Establishes policy... the S&S newspapers. (c) Authorizes the establishment, management, operation, and oversight of the... mission, production, distribution authority, and business operations as mission-essential activities of...

  7. 48 CFR 252.246-7004 - Safety of Facilities, Infrastructure, and Equipment for Military Operations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., Infrastructure, and Equipment for Military Operations. As prescribed in 246.270-4, use the following clause... contract comply with Unified Facilities Criteria (UFC) 1-200-01 for— (1) Fire protection; (2) Structural...

  8. 48 CFR 252.246-7004 - Safety of Facilities, Infrastructure, and Equipment for Military Operations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., Infrastructure, and Equipment for Military Operations. As prescribed in 246.270-4, use the following clause... contract comply with Unified Facilities Criteria (UFC) 1-200-01 for— (1) Fire protection; (2) Structural...

  9. 48 CFR 252.246-7004 - Safety of Facilities, Infrastructure, and Equipment for Military Operations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., Infrastructure, and Equipment for Military Operations. As prescribed in 246.270-4, use the following clause... contract comply with Unified Facilities Criteria (UFC) 1-200-01 for— (1) Fire protection; (2) Structural...

  10. 2,4,6-Trichlorophenol

    Integrated Risk Information System (IRIS)

    2,4,6 - Trichlorophenol ; CASRN 88 - 06 - 2 Human health assessment information on a chemical substance is included in the IRIS database only after a comprehensive review of toxicity data , as outlined in the IRIS assessment development process . Sections I ( Health Hazard Assessments for Noncarcino

  11. 48 CFR 246.103 - Contracting office responsibilities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Contracting office... SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE General 246.103 Contracting office responsibilities. (1) The contracting office must coordinate with the quality assurance activity before changing...

  12. COMPARISON OF BIOMARKERS IN WORKERS EXPOSED TO 2,4,6-TRINITROTOLUENE

    EPA Science Inventory

    2,4,6-Trinitrotoluene (TNT) is an important occupational and environmental pollutant. In TNT-exposed humans, the notable toxic manifestations have included aplastic anemia, toxic hepatitis, cataracts, hepatomegaly, and liver cancer. Therefore, we developed methods to biomonitor w...

  13. Solidification rate influence on orientation and mechanical properties of MAR-M-246+Hf

    NASA Technical Reports Server (NTRS)

    Hamilton, D.

    1983-01-01

    The influence of solidification rates on the orientation and mechanical properties of MAR-M-246+Hf was studied. The preferred orientation was found to be (001) for single crystals, with all samples with 45 degrees of (001). Tensile tests were performed at room temperature. The anisotropy of directionally solidified MAR-M-246+Hf was demonstrated by gage section deformation. Dendrite arm spacing and crystal growth were found to depend on solidification rates and source material conditions. The greatest strength occurred at lower solidification rates. Some single crystals were grown by control of growth rates without seeding.

  14. Metabolism of difebarbamate in man.

    PubMed

    Vachta, J; Valter, K; Siegfried, B

    1990-01-01

    The metabolism of 1,3-bis(3-butoxy-2-carbamoyloxypropyl)-5-ethyl-5-phenyl- (1H,3H,5H)-pyrimidine-2,4,6-trione (difebarbamate) in man was studied. Human volunteers received a single oral dose of 25 mg/kg difebarbamate. Urine was extracted with Amberlite XAD-2 resin and the extracts were separated by preparative HPLC after enzymatic hydrolysis. Four major metabolites were isolated and their structures were determined using NMR and mass spectrometry. The oxygen dealkylation led to the formation of two metabolites: 1-(3-butoxy-2-carbamoyloxypropyl)-3-(2-carbamoyloxy-3-hydrox ypropyl)-5-ethyl-5- phenyl-(1H, 3H, 5H)-pyrimidine-2,4,6,-trione and 1,3-bis(2-carbamoyloxy-3-hydroxypropyl)-5-ethyl-5-phenyl-(1H,3H,5H )- pyrimidine-2,4,6,-trione. The hydrolysis of the carbamoyloxy group with the oxygen dealkylation led to the formation of 1-(2-carbamoyloxy-3-hydroxypropyl)-3-(2,3-dihydroxypropyl)-5-ethyl - 5-phenyl-(1H,3H,5H)-pyrimidine-2,4,6,-tione, whereas the 4-hydroxylation of the benzene ring together with the oxygen dealkylation led to the formation of 1,3-bis(2-carbamoyloxy-3-hydroxypropyl)-5-ethyl-5-(4-hydroxyphenyl )-(1H,3H,5H)- pyrimidine-2,4,6,-trione. No traces of the parent drug were found.

  15. Monoterpene glycoside ESK246 from Pittosporum targets LAT3 amino acid transport and prostate cancer cell growth.

    PubMed

    Wang, Qian; Grkovic, Tanja; Font, Josep; Bonham, Sarah; Pouwer, Rebecca H; Bailey, Charles G; Moran, Anne M; Ryan, Renae M; Rasko, John E J; Jormakka, Mika; Quinn, Ronald J; Holst, Jeff

    2014-06-20

    The L-type amino acid transporter (LAT) family consists of four members (LAT1-4) that mediate uptake of neutral amino acids including leucine. Leucine is not only important as a building block for proteins, but plays a critical role in mTORC1 signaling leading to protein translation. As such, LAT family members are commonly upregulated in cancer in order to fuel increased protein translation and cell growth. To identify potential LAT-specific inhibitors, we established a function-based high-throughput screen using a prefractionated natural product library. We identified and purified two novel monoterpene glycosides, ESK242 and ESK246, sourced from a Queensland collection of the plant Pittosporum venulosum. Using Xenopus laevis oocytes expressing individual LAT family members, we demonstrated that ESK246 preferentially inhibits leucine transport via LAT3, while ESK242 inhibits both LAT1 and LAT3. We further show in LNCaP prostate cancer cells that ESK246 is a potent (IC50 = 8.12 μM) inhibitor of leucine uptake, leading to reduced mTORC1 signaling, cell cycle protein expression and cell proliferation. Our study suggests that ESK246 is a LAT3 inhibitor that can be used to study LAT3 function and upon which new antiprostate cancer therapies may be based.

  16. 48 CFR 246.402 - Government contract quality assurance at source.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Government contract... ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.402 Government contract quality assurance at source. Do not require...

  17. 48 CFR 2152.246-70 - Quality assurance requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Quality assurance... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CLAUSES AND FORMS PRECONTRACT PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2152.246-70 Quality assurance...

  18. 48 CFR 2152.246-70 - Quality assurance requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Quality assurance... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CLAUSES AND FORMS PRECONTRACT PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2152.246-70 Quality assurance...

  19. 48 CFR 2152.246-70 - Quality assurance requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Quality assurance... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CLAUSES AND FORMS PRECONTRACT PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2152.246-70 Quality assurance...

  20. 48 CFR 2152.246-70 - Quality assurance requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Quality assurance... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CLAUSES AND FORMS PRECONTRACT PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2152.246-70 Quality assurance...

  1. 48 CFR 2152.246-70 - Quality assurance requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Quality assurance... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CLAUSES AND FORMS PRECONTRACT PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2152.246-70 Quality assurance...

  2. 2,4,6-Trinitrotoluene (TNT)

    Integrated Risk Information System (IRIS)

    2,4,6 - Trinitrotoluene ( TNT ) ; CASRN 118 - 96 - 7 Human health assessment information on a chemical substance is included in the IRIS database only after a comprehensive review of toxicity data , as outlined in the IRIS assessment development process . Sections I ( Health Hazard Assessments for N

  3. 48 CFR 246.472 - Inspection stamping.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.472 Inspection stamping. (a) DoD quality inspection approval marking designs (stamps) may be used for both prime...) Policies and procedures regarding the use of National Aeronautics and Space Administration (NASA) quality...

  4. 7 CFR 246.19 - Management evaluation and monitoring reviews.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN Monitoring and Review § 246.19 Management evaluation and monitoring reviews... reports, the development of corrective action plans to resolve Program deficiencies, the monitoring of the...

  5. 7 CFR 246.19 - Management evaluation and monitoring reviews.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN Monitoring and Review § 246.19 Management evaluation and monitoring reviews... reports, the development of corrective action plans to resolve Program deficiencies, the monitoring of the...

  6. 7 CFR 246.19 - Management evaluation and monitoring reviews.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN Monitoring and Review § 246.19 Management evaluation and monitoring reviews... reports, the development of corrective action plans to resolve Program deficiencies, the monitoring of the...

  7. 7 CFR 246.19 - Management evaluation and monitoring reviews.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN Monitoring and Review § 246.19 Management evaluation and monitoring reviews... reports, the development of corrective action plans to resolve Program deficiencies, the monitoring of the...

  8. 7 CFR 246.19 - Management evaluation and monitoring reviews.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN Monitoring and Review § 246.19 Management evaluation and monitoring reviews... reports, the development of corrective action plans to resolve Program deficiencies, the monitoring of the...

  9. 7 CFR 246.28 - OMB control numbers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN... information collection requirements in 7 CFR part 246 by the Office of Management and Budget pursuant to the...

  10. 7 CFR 246.28 - OMB control numbers.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN... information collection requirements in 7 CFR part 246 by the Office of Management and Budget pursuant to the...

  11. 7 CFR 246.28 - OMB control numbers.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN... information collection requirements in 7 CFR part 246 by the Office of Management and Budget pursuant to the...

  12. 7 CFR 246.28 - OMB control numbers.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN... information collection requirements in 7 CFR part 246 by the Office of Management and Budget pursuant to the...

  13. 7 CFR 246.28 - OMB control numbers.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN... information collection requirements in 7 CFR part 246 by the Office of Management and Budget pursuant to the...

  14. 48 CFR 52.246-1 - Contractor Inspection Requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Contractor Inspection....246-1 Contractor Inspection Requirements. As prescribed in 46.301, insert the following clause: Contractor Inspection Requirements (APR 1984) The Contractor is responsible for performing or having...

  15. Who Guards the Guards: Drug Use Pattern Among Medical Students in a Nigerian University

    PubMed Central

    Babalola, EO; Akinhanmi, A; Ogunwale, A

    2014-01-01

    Background: Several studies have examined the prevalence and pattern of substance use among medical students in Nigeria. Few of these studies have specifically examined the relationship between the psychological distress and psychoactive substance use among these students. Yet, evidence world-wide suggests that substance use among medical students might be on the rise and may be related to the level of stress among them. Aim: The present study is the first study aimed to determine the prevalence, pattern and factors associated with psychoactive substance use among medical students of Olabisi Onabanjo University, Ogun State, Nigeria. Subjects and Methods: The World Health Organization student drug use questionnaire was used to evaluate for substance use among 246 clinical medical students between September and October 2011. General health questionnaire (GHQ) 12 was used to assess for psychological distress among these students. Statistical analysis was performed using the SPSS version 16. (Chicago, USA). Proportions were compared using the Chi-square test while a value of P < 0.05 was considered statistically significant. Fisher exact test was used instead of Chi-square when the number in the cell is less than 5. Results: Lifetime prevalence of substance use among medical students was 65% (165/246). It was found that the most commonly used substances were alcohol 63.4% (156/246), mild stimulants 15.6% (38/246), tobacco 15% (37/246) and sedatives 6.1% (15/246). Substance use was associated with gender, frequency of participation in religious activities and GHQ scores. Conclusion: Psychoactive substance use is a major problem among medical students. Psychological well-being plays a significant role in substance use among these students. There is a need for adequate screening and assessment for substance use disorders among these students and incorporating stress management strategies in their curriculum. PMID:24971216

  16. Characterization of a Highly Enriched Microbial Consortium Reductively Dechlorinating 2,3-Dichlorophenol and 2,4,6-Trichlorophenol and the Corresponding cprA Genes from River Sediment.

    PubMed

    El-Sayed, Wael S

    2016-08-26

    Anaerobic reductive dechlorination of 2,3-dichlorophenol (2,3DCP) and 2,4,6-trichlorophenol (2,4,6TCP) was investigated in microcosms from River Nile sediment. A stable sediment-free anaerobic microbial consortium reductively dechlorinating 2,3DCP and 2,4,6TCP was established. Defined sediment-free cultures showing stable dechlorination were restricted to ortho chlorine when enriched with hydrogen as the electron donor, acetate as the carbon source, and either 2,3-DCP or 2,4,6-TCP as electron acceptors. When acetate, formate, or pyruvate were used as electron donors, dechlorination activity was lost. Only lactate can replace dihydrogen as an electron donor. However, the dechlorination potential was decreased after successive transfers. To reveal chlororespiring species, the microbial community structure of chlorophenol-reductive dechlorinating enrichment cultures was analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. Eight dominant bacteria were detected in the dechlorinating microcosms including members of the genera Citrobacter, Geobacter, Pseudomonas, Desulfitobacterium, Desulfovibrio and Clostridium. Highly enriched dechlorinating cultures were dominated by four bacterial species belonging to the genera Pseudomonas, Desulfitobacterium, and Clostridium. Desulfitobacterium represented the major fraction in DGGE profiles indicating its importance in dechlorination activity, which was further confirmed by its absence resulting in complete loss of dechlorination. Reductive dechlorination was confirmed by the stoichiometric dechlorination of 2,3DCP and 2,4,6TCP to metabolites with less chloride groups and by the detection of chlorophenol RD cprA gene fragments in dechlorinating cultures. PCR amplified cprA gene fragments were cloned and sequenced and found to cluster with the cprA/pceA type genes of Dehalobacter restrictus.

  17. 7 CFR 246.22 - Administrative appeal of FNS decisions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 246.22 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS... Agriculture, Food and Nutrition Service, 3101 Park Center Drive, Alexandria, Va. 22302, for a hearing or a...

  18. 48 CFR 246.470-2 - Quality evaluation data.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Quality evaluation data... SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.470-2 Quality evaluation data. The contract administration office shall establish a system for the...

  19. 7 CFR 246.22 - Administrative appeal of FNS decisions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 246.22 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS... Agriculture, Food and Nutrition Service, 3101 Park Center Drive, Alexandria, Va. 22302, for a hearing or a...

  20. 40 CFR 86.246-94 - Intermediate temperature testing.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 19 2013-07-01 2013-07-01 false Intermediate temperature testing. 86... New Medium-Duty Passenger Vehicles; Cold Temperature Test Procedures § 86.246-94 Intermediate temperature testing. (a) This section is applicable to tests which are conducted at an intermediate...

  1. 40 CFR 86.246-94 - Intermediate temperature testing.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 19 2012-07-01 2012-07-01 false Intermediate temperature testing. 86... New Medium-Duty Passenger Vehicles; Cold Temperature Test Procedures § 86.246-94 Intermediate temperature testing. (a) This section is applicable to tests which are conducted at an intermediate...

  2. 40 CFR 86.246-94 - Intermediate temperature testing.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 18 2011-07-01 2011-07-01 false Intermediate temperature testing. 86... New Medium-Duty Passenger Vehicles; Cold Temperature Test Procedures § 86.246-94 Intermediate temperature testing. (a) This section is applicable to tests which are conducted at an intermediate...

  3. 42 CFR 24.6 - Pay and compensation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... United States under title 5, United States Code. ... RESEARCH SERVICE § 24.6 Pay and compensation. The SBRS is an ungraded system, with a single, flexible pay... appointees to the Service, who are not covered by the Civil Service Retirement System, will be covered by the...

  4. SOIL REDUCES 2,4,6-TRINITROTOLUENE BIOAVAILABILITY AND GENOTOXICITY IN VIVO

    EPA Science Inventory

    Soil Reduces 2,4,6- Trinitrotoluene Bioavailability and Genotoxicity In Vivo

    As chemicals age in soil, irreversible binding of chemical contaminants to organic components in the soil may occur, impacting the amount of chemical that is biologically available upon exp...

  5. Peptides Labeled with Pyridinium Salts for Sensitive Detection and Sequencing by Electrospray Tandem Mass Spectrometry

    NASA Astrophysics Data System (ADS)

    Waliczek, Mateusz; Kijewska, Monika; Rudowska, Magdalena; Setner, Bartosz; Stefanowicz, Piotr; Szewczuk, Zbigniew

    2016-11-01

    Mass spectrometric analysis of trace amounts of peptides may be problematic due to the insufficient ionization efficiency resulting in limited sensitivity. One of the possible ways to overcome this problem is the application of ionization enhancers. Herein we developed new ionization markers based on 2,4,6-triphenylpyridinium and 2,4,6-trimethylpyridinium salts. Using of inexpensive and commercially available pyrylium salt allows selective derivatization of primary amino groups, especially those sterically unhindered, such as ɛ-amino group of lysine. The 2,4,6-triphenylpyridinium modified peptides generate in MS/MS experiments an abundant protonated 2,4,6-triphenylpyridinium ion. This fragment is a promising reporter ion for the multiple reactions monitoring (MRM) analysis. In addition, the fixed positive charge of the pyridinium group enhances the ionization efficiency. Other advantages of the proposed ionization enhancers are the simplicity of derivatization of peptides and the possibility of convenient incorporation of isotopic labels into derivatized peptides.

  6. Hybrid treatment strategies for 2,4,6-trichlorophenol degradation based on combination of hydrodynamic cavitation and AOPs.

    PubMed

    Barik, Arati J; Gogate, Parag R

    2018-01-01

    Utilization of hybrid treatment schemes involving advanced oxidation processes and hydrodynamic cavitation in the wastewater treatment forms the prime focus of the present work. The initial phase of the work includes analysis of recent literature relating to the performance of combined approach based on hydrodynamic cavitation (HC) for degradation of different pollutants followed by a detailed investigation into degradation of 2,4,6-trichlorophenol (2,4,6-TCP). The degradation of the priority pollutant, 2,4,6-TCP, using combination of HC based on slit-venturi used as the cavitating device, ozone and H 2 O 2 has been investigated. The effect of operating pressure (2-5bar) and initial pH (3-11) have been investigated for the degradation using only HC. The degradation using only ozone (100-400mg/h) and only H 2 O 2 has also been studied. The efficacy of the combined operation of HC+O 3 at different ozone flow rates (100-400mg/h) and the combined operation of HC+H 2 O 2 at different loadings of H 2 O 2 (2,4,6-TCP:H 2 O 2 as 1:1-1:7) have been subsequently investigated. The degradation efficacy has also been established for the combined treatment strategies of O 3 +H 2 O 2 and HC+O 3 +H 2 O 2 at the optimum conditions of temperature as 30°C, inlet pressure of 4bar and initial pH of 7. Extent of 2,4,6-TCP degradation, TOC and COD removal obtained for HC+O 3 process were 97.1%, 94.4% and 78.5% respectively whereas for O 3 +H 2 O 2 process, the values were 95.5%, 94.8% and 76.2% and for HC+O 3 +H 2 O 2 process the extent of reduction were 100%, 95.6% and 80.9% in the same order. The combined treatment approach as HC+O 3 +H 2 O 2 was established as the most efficient approach for complete removal of 2,4,6-TCP with near complete TOC removal. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Cyclic voltammetry: a tool to quantify 2,4,6-trichloroanisole in aqueous samples from cork planks boiling industrial process.

    PubMed

    Peres, António M; Freitas, Patrícia; Dias, Luís G; Sousa, Mara E B C; Castro, Luís M; Veloso, Ana C A

    2013-12-15

    Chloroanisoles, namely 2,4,6-trichloroanisole, are pointed out as the primary responsible of the development of musty off-flavours in bottled wine, due to their migration from cork stoppers, which results in huge economical losses for wine industry. A prevention step is the detection of these compounds in cork planks before stoppers are produced. Mass spectrometry gas chromatography is the reference method used although it is far beyond economical possibilities of the majority of cork stoppers producers. In this work, a portable cyclic voltammetry approach was used to detect 2,4,6-trichloroanisole extracted from natural cork planks to the aqueous phase during the cork boiling industrial treatment process. Analyses were carried out under ambient conditions, in less than 15 min with a low use of solvent and without any sample pre-treatment. The proposed technique had detection (0.31±0.01 ng/L) and quantification (0.95±0.05 ng/L) limits lower than the human threshold detection level. For blank solutions, without 2,4,6-trichloroanisole addition, a concentration in the order of the quantification limit was estimated (1.0±0.2 ng/L), which confirms the satisfactory performance of the proposed methodology. For aqueous samples from the industrial cork planks boiling procedure, intra-day repeatabilities were lower than 3%, respectively. Also, 2,4,6-trichloroanisole contents in the aqueous samples determined by this novel approach were in good agreement with those obtained by GC-MS (correlation coefficient equal to 0.98), confirming the satisfactory accuracy of the proposed methodology. So, since this novel approach is a fast, low-cost, portable and user-friendly method, it can be an alternative and helpful tool for in-situ industrial applications, allowing accurate detection of releasable 2,4,6-trichloroanisole in an earlier phase of cork stoppers production, which may allow implementing more effective cork treatments to reduce or avoid future 2,4,6-trichloroanisole contaminations of wine. © 2013 Elsevier B.V. All rights reserved.

  8. 12 CFR 24.6 - Examples of qualifying public welfare investments.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 1 2012-01-01 2012-01-01 false Examples of qualifying public welfare... COMMUNITY AND ECONOMIC DEVELOPMENT ENTITIES, COMMUNITY DEVELOPMENT PROJECTS, AND OTHER PUBLIC WELFARE INVESTMENTS § 24.6 Examples of qualifying public welfare investments. Investments that primarily support the...

  9. 12 CFR 24.6 - Examples of qualifying public welfare investments.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 1 2014-01-01 2014-01-01 false Examples of qualifying public welfare... COMMUNITY AND ECONOMIC DEVELOPMENT ENTITIES, COMMUNITY DEVELOPMENT PROJECTS, AND OTHER PUBLIC WELFARE INVESTMENTS § 24.6 Examples of qualifying public welfare investments. Investments that primarily support the...

  10. 32 CFR 246.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.3 Definitions. (a) Adverse Conditions... publisher of the Stars and Stripes produced by that organization. (c) S&S Management Action Group (MAG) and..., involve themselves in Stars and Stripes editorial policies. (d) S&S Ombudsman. A highly qualified...

  11. 32 CFR 246.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.3 Definitions. (a) Adverse Conditions... publisher of the Stars and Stripes produced by that organization. (c) S&S Management Action Group (MAG) and..., involve themselves in Stars and Stripes editorial policies. (d) S&S Ombudsman. A highly qualified...

  12. 32 CFR 246.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.3 Definitions. (a) Adverse Conditions... publisher of the Stars and Stripes produced by that organization. (c) S&S Management Action Group (MAG) and..., involve themselves in Stars and Stripes editorial policies. (d) S&S Ombudsman. A highly qualified...

  13. 7 CFR 246.22 - Administrative appeal of FNS decisions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN Miscellaneous Provisions § 246.22 Administrative appeal of FNS decisions. (a) Right to... position when a hearing is involved; and (vii) A list of prospective State agency witnesses when a hearing...

  14. 2,4,6-TRINITROTOLUENE TRANSFORMATION IN PLANTED, SOIL COLUMNS

    EPA Science Inventory

    2,4,6-Trinitrotoluene (TNT), has been used extensively by the United States military to manufacture explosive devices. Since the conclusion of World War 11, many of these military installations have also been involved in the disposal of surplus and outdated explosives. Both the c...

  15. SAGE ANALYSIS OF TRANSCRIPTOME RESPONSES IN ARABIDOPSIS ROOTS EXPOSED TO 2,4,6-TRINITROTOLUENE

    EPA Science Inventory

    Serial Analysis of Gene Expression (SAGE) was used to profile transcript levels in Arabidopsis thaliana roots and assess their responses to 2,4,6-trinitrotoluene (TNT) exposure. SAGE libraries representing control and TNT-exposed seedling root transcripts were constructed, and ea...

  16. 40 CFR 86.246-94 - Intermediate temperature testing.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM NEW AND IN-USE HIGHWAY VEHICLES AND ENGINES Emission Regulations for 1994 and Later Model Year Gasoline-Fueled New Light-Duty Vehicles, New Light-Duty Trucks and New Medium-Duty Passenger Vehicles; Cold Temperature Test Procedures § 86.246-94 Intermediate...

  17. 40 CFR 246.200-6 - Recommended procedures: Storage.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Storage. 246....200-6 Recommended procedures: Storage. Among the alternatives for paper storage are on-site bailing, the use of stationary compactors, or storage in corrugated boxes or normal waste containers. Stored...

  18. 40 CFR 246.200-6 - Recommended procedures: Storage.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Recommended procedures: Storage. 246....200-6 Recommended procedures: Storage. Among the alternatives for paper storage are on-site bailing, the use of stationary compactors, or storage in corrugated boxes or normal waste containers. Stored...

  19. 40 CFR 246.200-6 - Recommended procedures: Storage.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Recommended procedures: Storage. 246....200-6 Recommended procedures: Storage. Among the alternatives for paper storage are on-site bailing, the use of stationary compactors, or storage in corrugated boxes or normal waste containers. Stored...

  20. 40 CFR 246.200-6 - Recommended procedures: Storage.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Recommended procedures: Storage. 246....200-6 Recommended procedures: Storage. Among the alternatives for paper storage are on-site bailing, the use of stationary compactors, or storage in corrugated boxes or normal waste containers. Stored...

  1. 40 CFR 246.200-6 - Recommended procedures: Storage.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recommended procedures: Storage. 246....200-6 Recommended procedures: Storage. Among the alternatives for paper storage are on-site bailing, the use of stationary compactors, or storage in corrugated boxes or normal waste containers. Stored...

  2. 48 CFR 246.407 - Nonconforming supplies or services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 246.407 Nonconforming supplies or services. (f) If nonconforming material or services are discovered... writing of the nonconforming material or service; (ii) Shall request that the contractor repair or replace the material, or perform the service, at no cost to the Government; and (iii) May accept consideration...

  3. 48 CFR 246.407 - Nonconforming supplies or services.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 246.407 Nonconforming supplies or services. (f) If nonconforming material or services are discovered... writing of the nonconforming material or service; (ii) Shall request that the contractor repair or replace the material, or perform the service, at no cost to the Government; and (iii) May accept consideration...

  4. 48 CFR 246.407 - Nonconforming supplies or services.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 246.407 Nonconforming supplies or services. (f) If nonconforming material or services are discovered... writing of the nonconforming material or service; (ii) Shall request that the contractor repair or replace the material, or perform the service, at no cost to the Government; and (iii) May accept consideration...

  5. 48 CFR 246.407 - Nonconforming supplies or services.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 246.407 Nonconforming supplies or services. (f) If nonconforming material or services are discovered... writing of the nonconforming material or service; (ii) Shall request that the contractor repair or replace the material, or perform the service, at no cost to the Government; and (iii) May accept consideration...

  6. 8 CFR 246.4 - Immigration judge's authority; withdrawal and substitution.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Immigration judge's authority; withdrawal... IMMIGRATION REGULATIONS RESCISSION OF ADJUSTMENT OF STATUS § 246.4 Immigration judge's authority; withdrawal and substitution. In any proceeding conducted under this part, the immigration judge shall have...

  7. Monoterpene Glycoside ESK246 from Pittosporum Targets LAT3 Amino Acid Transport and Prostate Cancer Cell Growth

    PubMed Central

    2014-01-01

    The l-type amino acid transporter (LAT) family consists of four members (LAT1–4) that mediate uptake of neutral amino acids including leucine. Leucine is not only important as a building block for proteins, but plays a critical role in mTORC1 signaling leading to protein translation. As such, LAT family members are commonly upregulated in cancer in order to fuel increased protein translation and cell growth. To identify potential LAT-specific inhibitors, we established a function-based high-throughput screen using a prefractionated natural product library. We identified and purified two novel monoterpene glycosides, ESK242 and ESK246, sourced from a Queensland collection of the plant Pittosporum venulosum. Using Xenopus laevis oocytes expressing individual LAT family members, we demonstrated that ESK246 preferentially inhibits leucine transport via LAT3, while ESK242 inhibits both LAT1 and LAT3. We further show in LNCaP prostate cancer cells that ESK246 is a potent (IC50 = 8.12 μM) inhibitor of leucine uptake, leading to reduced mTORC1 signaling, cell cycle protein expression and cell proliferation. Our study suggests that ESK246 is a LAT3 inhibitor that can be used to study LAT3 function and upon which new antiprostate cancer therapies may be based. PMID:24762008

  8. Florida School Laws. Chapters 228-246 Florida Statutes. 1998 Edition.

    ERIC Educational Resources Information Center

    Florida State Dept. of Education, Tallahassee.

    This volume of Florida School Laws contains chapters 228 through 246 of the Florida Statutes, which comprise "The Florida School Code." The laws contain those statutes specifically applicable to public schools, community colleges, postsecondary institutions, all other institutions and agencies included as a part of the state system of…

  9. 30 CFR 550.246 - What mineral resource conservation information must accompany the DPP or DOCD?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... SHELF Plans and Information Contents of Development and Production Plans (dpp) and Development Operations Coordination Documents (docd) § 550.246 What mineral resource conservation information must... 30 Mineral Resources 2 2013-07-01 2013-07-01 false What mineral resource conservation information...

  10. 30 CFR 550.246 - What mineral resource conservation information must accompany the DPP or DOCD?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... SHELF Plans and Information Contents of Development and Production Plans (dpp) and Development Operations Coordination Documents (docd) § 550.246 What mineral resource conservation information must... 30 Mineral Resources 2 2014-07-01 2014-07-01 false What mineral resource conservation information...

  11. 30 CFR 550.246 - What mineral resource conservation information must accompany the DPP or DOCD?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... SHELF Plans and Information Contents of Development and Production Plans (dpp) and Development Operations Coordination Documents (docd) § 550.246 What mineral resource conservation information must... 30 Mineral Resources 2 2012-07-01 2012-07-01 false What mineral resource conservation information...

  12. 48 CFR 1852.246-70 - Mission Critical Space System Personnel Reliability Program.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Mission Critical Space... CONTRACT CLAUSES Texts of Provisions and Clauses 1852.246-70 Mission Critical Space System Personnel Reliability Program. As prescribed in 1846.370(a), insert the following clause: Mission Critical Space System...

  13. 48 CFR 1852.246-70 - Mission Critical Space System Personnel Reliability Program.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Mission Critical Space... CONTRACT CLAUSES Texts of Provisions and Clauses 1852.246-70 Mission Critical Space System Personnel Reliability Program. As prescribed in 1846.370(a), insert the following clause: Mission Critical Space System...

  14. 48 CFR 1852.246-70 - Mission Critical Space System Personnel Reliability Program.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Mission Critical Space... CONTRACT CLAUSES Texts of Provisions and Clauses 1852.246-70 Mission Critical Space System Personnel Reliability Program. As prescribed in 1846.370(a), insert the following clause: Mission Critical Space System...

  15. 48 CFR 1852.246-70 - Mission Critical Space System Personnel Reliability Program.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Mission Critical Space... CONTRACT CLAUSES Texts of Provisions and Clauses 1852.246-70 Mission Critical Space System Personnel Reliability Program. As prescribed in 1846.370(a), insert the following clause: Mission Critical Space System...

  16. 48 CFR 1852.246-70 - Mission Critical Space System Personnel Reliability Program.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Mission Critical Space... CONTRACT CLAUSES Texts of Provisions and Clauses 1852.246-70 Mission Critical Space System Personnel Reliability Program. As prescribed in 1846.370(a), insert the following clause: Mission Critical Space System...

  17. The Preparation and Reaction of Phenyl-Substituted Pyrylium and Pyridinium Salts.

    ERIC Educational Resources Information Center

    Awartani, Radi; And Others

    1986-01-01

    Describes this reaction sequence involving reactivity and synthesis of heterocycles: (1) synthesis of 2,4,6-triphenylpyrylium tetrafluoroborate, II; (2) its reaction with nucleophiles; (3) reaction of pyrylium salt II with a primary amine (benzylamine, p-methoxybenzylamine, and furfurylamine) to form the N-substituted-2,4,6-triphenylpyridinium…

  18. 7 CFR 246.16a - Infant formula and authorized foods cost containment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN State Agency Provisions § 246.16a Infant formula and authorized foods... 7 Agriculture 4 2014-01-01 2014-01-01 false Infant formula and authorized foods cost containment...

  19. 7 CFR 246.16a - Infant formula and authorized foods cost containment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN State Agency Provisions § 246.16a Infant formula and authorized foods... 7 Agriculture 4 2013-01-01 2013-01-01 false Infant formula and authorized foods cost containment...

  20. 7 CFR 246.16a - Infant formula and authorized foods cost containment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL SUPPLEMENTAL NUTRITION PROGRAM FOR WOMEN, INFANTS AND CHILDREN State Agency Provisions § 246.16a Infant formula and authorized foods... 7 Agriculture 4 2012-01-01 2012-01-01 false Infant formula and authorized foods cost containment...

  1. 32 CFR 246.5 - Responsibilities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.5 Responsibilities. (a) The Assistant to... Command, shall: (1) Authorize a Stars and Stripes newspaper, provide operational direction to the S&S... published in the Stars and Stripes. (4) Select the S&S commander/publisher and other military officers in S...

  2. 32 CFR 246.5 - Responsibilities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.5 Responsibilities. (a) The Assistant to... Command, shall: (1) Authorize a Stars and Stripes newspaper, provide operational direction to the S&S... published in the Stars and Stripes. (4) Select the S&S commander/publisher and other military officers in S...

  3. 32 CFR 246.5 - Responsibilities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS § 246.5 Responsibilities. (a) The Assistant to... Command, shall: (1) Authorize a Stars and Stripes newspaper, provide operational direction to the S&S... published in the Stars and Stripes. (4) Select the S&S commander/publisher and other military officers in S...

  4. 48 CFR 246.710 - Contract clauses.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... contracts that include the clause at 252.227-7013, Rights in Technical Data and Computer Software, when... Alternate I when extended liability is desired and a fixed price incentive contract is contemplated. (3) Use... and a firm fixed price contract is contemplated. (4) Use the clause at 252.246-7002, Warranty of...

  5. 12. Photographic copy of construction drawing, dated September 29, 1932, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Photographic copy of construction drawing, dated September 29, 1932, Construction Division Office of the Quartermaster General, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. PLAN 625-25?? SDO 163 (ELEVATIONS) - Selfridge Field, Building Nos. 246 & 253, 246 Birch Street & 253 Wagner Street, Mount Clemens, Macomb County, MI

  6. USING ROTIFER POPULATION DEMOGRAPHIC PARAMETERS TO ASSESS IMPACTS OF THE DEGRADATION PRODUCTS FROM TRINITROTOLUENE PHYTOREMEDIATION

    EPA Science Inventory

    The objective of this study was to evaluate the aquatic chronic lethal and sublethal toxicity effects from the phytoremediation of water contaminated with 2,4,6-trinitrotoluene (2,4,6-TNT) by the wetland plant species Myriophyllum aquaticum (parrot feather). Rotifers (Brachionus...

  7. 40 CFR 180.441 - Quizalofop ethyl; tolerances for residues.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... combined residues of the herbicide quizalofop (2-[4-(6-chloroquinoxalin-2-yl oxy)phenoxy]propanoic acid... combined residues of the herbicide quizalofop (2-[4-(6-chloroquinoxalin-2-yl oxy)phenoxy]propanoic acid... byproducts 0.05 (3) Tolerances are established for the combined residues of the herbicide quizalofop-p ethyl...

  8. 40 CFR 180.441 - Quizalofop ethyl; tolerances for residues.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... combined residues of the herbicide quizalofop (2-[4-(6-chloroquinoxalin-2-yl oxy)phenoxy]propanoic acid... combined residues of the herbicide quizalofop (2-[4-(6-chloroquinoxalin-2-yl oxy)phenoxy]propanoic acid... byproducts 0.05 (3) Tolerances are established for the combined residues of the herbicide quizalofop-p ethyl...

  9. 40 CFR 721.10279 - Multi-walled carbon nanotubes (generic) (P-10-246).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) TOXIC SUBSTANCES CONTROL ACT SIGNIFICANT NEW USES OF CHEMICAL SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.10279 Multi-walled carbon nanotubes (generic) (P-10-246). (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically...

  10. 40 CFR 246.200-4 - Recommended procedures: Levels of separation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recommended procedures: Levels of... § 246.200-4 Recommended procedures: Levels of separation. A two-level separation is recommended for most... separate category cost effective may choose to implement three levels of separation: (1) Computer papers...

  11. 7 CFR 246.23 - Claims and penalties.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... through a review of the State agency's reports, program or financial analysis, monitoring, audit, or... benefit analysis, for determining when collection actions are no longer cost-effective. At the time the... required by § 246.12(u)(2). (ii) Types of restitution. In lieu of financial restitution, the State agency...

  12. 7 CFR 246.23 - Claims and penalties.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... through a review of the State agency's reports, program or financial analysis, monitoring, audit, or... benefit analysis, for determining when collection actions are no longer cost-effective. At the time the... required by § 246.12(u)(2). (ii) Types of restitution. In lieu of financial restitution, the State agency...

  13. 30 CFR 250.246 - What mineral resource conservation information must accompany the DPP or DOCD?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... OPERATIONS IN THE OUTER CONTINENTAL SHELF Plans and Information Contents of Development and Production Plans (dpp) and Development Operations Coordination Documents (docd) § 250.246 What mineral resource... information, as applicable, must accompany your DPP or DOCD: (a) Technology and reservoir engineering...

  14. 40 CFR 246.201-7 - Recommended procedures: Cost analysis.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Cost analysis... § 246.201-7 Recommended procedures: Cost analysis. After potential markets have been located (but prior... residual solid waste have been established, an analysis should be conducted which compares the costs of the...

  15. 40 CFR 246.202-6 - Recommended procedures: Cost analysis.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Cost analysis... § 246.202-6 Recommended procedures: Cost analysis. After potential markets have been identified (but... residual solid waste have been established, an analysis should be conducted which compares the costs of the...

  16. 40 CFR 246.200-8 - Recommended procedures: Cost analysis.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Cost analysis... § 246.200-8 Recommended procedures: Cost analysis. After potential markets have been located (but prior... paper and residual solid waste have been established, an analysis should be conducted which compares the...

  17. 12 CFR 19.246 - Petition for reinstatement.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... PROCEDURE Removal, Suspension, and Debarment of Accountants From Performing Audit Services § 19.246 Petition... reinstatement by an independent public accountant, an accounting firm, or an office of a firm that was removed... the Comptroller, be afforded a hearing. The accountant or firm shall bear the burden of going forward...

  18. 12 CFR 19.246 - Petition for reinstatement.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... PROCEDURE Removal, Suspension, and Debarment of Accountants From Performing Audit Services § 19.246 Petition... reinstatement by an independent public accountant, an accounting firm, or an office of a firm that was removed... the Comptroller, be afforded a hearing. The accountant or firm shall bear the burden of going forward...

  19. 12 CFR 19.246 - Petition for reinstatement.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... PROCEDURE Removal, Suspension, and Debarment of Accountants From Performing Audit Services § 19.246 Petition... reinstatement by an independent public accountant, an accounting firm, or an office of a firm that was removed... the Comptroller, be afforded a hearing. The accountant or firm shall bear the burden of going forward...

  20. 12 CFR 19.246 - Petition for reinstatement.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... PROCEDURE Removal, Suspension, and Debarment of Accountants From Performing Audit Services § 19.246 Petition... reinstatement by an independent public accountant, an accounting firm, or an office of a firm that was removed... the Comptroller, be afforded a hearing. The accountant or firm shall bear the burden of going forward...

  1. Combined Therapy with Rheum tanguticum Polysaccharide and Low-dose 5-ASA Ameliorates TNBS-Induced Colitis in Rats by Suppression of NF-κB.

    PubMed

    Liu, Linna; Liu, Zhenxiong; Zhang, Tian; Shi, Lei; Zhang, Wenjuan; Zhang, Yan

    2015-06-01

    The most common conventional therapy for inflammatory bowel disease in clinical practice involves the use of nonsteroidal anti-inflammatory drugs, such as 5-amino salicylic acid. However, a high dose of 5-amino salicylic acid may bring about severe side effects. Chinese people have used Rheum tanguticum as a folk remedy for gastrointestinal disease for two thousand years. Our group has isolated R. tanguticum polysaccharide 1 from R. tanguticum and verified that it can attenuate 2,4,6-trinitrobenzene sulfonic acid-induced colitis in murines/rats. The present study aims to evaluate whether the addition of R. tanguticum polysaccharide 1 can improve efficacy and limit subsequent side effects of conventional treatment (5-amino salicylic acid) in rats with 2,4,6-trinitrobenzene sulfonic acid-induced colitis. Sixty Sprague-Dawley male rats were randomized into five groups and treated with (1) saline (saline, 0.2 mL/day × 5, p. o.), (2) 2,4,6-trinitrobenzene sulfonic acid alone (saline, 0.2 mL/day × 5, p. o.), (3) 2,4,6-trinitrobenzene sulfonic acid + 5-amino salicylic acid (5-amino salicylic acid, 75 mg/kg/day × 5, p.o), (4) 2,4,6-trinitrobenzene sulfonic acid + R. tanguticum polysaccharide 1 (R. tanguticum polysaccharide 1, 200 mg/kg/day × 5, p. o.), and (5) 2,4,6-trinitrobenzene sulfonic acid + 5-amino salicylic acid + R. tanguticum polysaccharide 1 (5-amino salicylic acid, 25 mg/kg/day × 5, p.o; R. tanguticum polysaccharide 1, 200 mg/kg/day × 5, p. o.). All the rats were sacrificed on the 6th day after treatment using an overdose of anesthesia. A histological assessment was performed using semiquantitative scores; nuclear factor-kappa B and tumor necrosis factor-α were measured with Western blot, cyclooxygenase 1 and cyclooxygenase 2 protein expressions were investigated by RT-polymerase chain reaction, and prostoglandin E2 and inducible nitric oxide synthase productions were investigated by ELISA. The extent and severity of histological signs were attenuated significantly in the 2,4,6-trinitrobenzene sulfonic acid + 5-amino salicylic acid + R. tanguticum polysaccharide 1 group. Treatment with R. tanguticum polysaccharide 1 plus 5-amino salicylic acid markedly decreased nuclear factor-kappa Bp65 and tumor necrosis factor-α protein expressions. R. tanguticum polysaccharide 1 and 5-amino salicylic acid had no effect on cyclooxygenase 1 protein expression, but inhibited the overexpression of the cyclooxygenase 2 protein. After treatment with 5-amino salicylic acid and R. tanguticum polysaccharide 1, the prostoglandin E2 level increased significantly and the inducible nitric oxide synthase level decreased considerably in the 2,4,6-trinitrobenzene sulfonic acid + 5-amino salicylic acid + R. tanguticum polysaccharide 1 group compared with the 2,4,6-trinitrobenzene sulfonic acid alone group. These results demonstrate that combined therapy with R. tanguticum polysaccharide 1 and low-dose 5-amino salicylic acid had more favorable effects on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats, and its effects may be associated with inhibiting nuclear factor-kappa Bp65 protein expression and tumor necrosis factor-α production, resulting in a decrease of cyclooxygenase 2 and inducible nitric oxide synthase protein expressions. Georg Thieme Verlag KG Stuttgart · New York.

  2. Amination of electrophilic aromatic compounds by vicarious nucleophilic substitution

    DOEpatents

    Mitchell, Alexander R.; Pagoria, Philip F.; Schmidt, Robert D.

    2000-01-01

    The present invention relates to a process to aminate electrophilic aromatic compounds by vicarious nucleophilic substitution of hydrogen using quaternary hydrazinium salts. The use of trialkylhydrazinium halide, e.g., trimethylhydrazinium iodide, as well as hydroxylamine, alkoxylamines, and 4-amino-1,2,4-triazole to produce aminated aromatic structures, such as 1,3-diamino-2,4,6-trinitrobenzene (DATB), 1,3,5-triamino-2,4,6-trinitrobenzene (TATB) and 3,5-diamino-2,4,6-trinitrotoluene (DATNT), is described. DATB and TATB are useful insensitive high explosives. TATB is also used for the preparation of benzenehexamine, a starting material for the synthesis of novel materials (optical imaging devices, liquid crystals, ferromagnetic compounds).

  3. 32 CFR Appendix C to Part 246 - Personnel Policies and Procedures

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS Pt. 246, App. C Appendix... Policies. 1. The Stars and Stripes (S&S) shall have a personnel system that is business oriented in terms... coordination with the Military Services and the Unified Commands. D. Enlisted Members of the Stars and Stripes...

  4. 32 CFR Appendix C to Part 246 - Personnel Policies and Procedures

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS Pt. 246, App. C Appendix... Policies. 1. The Stars and Stripes (S&S) shall have a personnel system that is business oriented in terms... coordination with the Military Services and the Unified Commands. D. Enlisted Members of the Stars and Stripes...

  5. 40 CFR 246.201-3 - Recommended procedures: Glass, can, and mixed paper separation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recommended procedures: Glass, can... and Recommended Procedures § 246.201-3 Recommended procedures: Glass, can, and mixed paper separation. In areas where markets are available, it is recommended that glass, cans, and mixed paper be...

  6. 40 CFR 246.201-3 - Recommended procedures: Glass, can, and mixed paper separation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recommended procedures: Glass, can... and Recommended Procedures § 246.201-3 Recommended procedures: Glass, can, and mixed paper separation. In areas where markets are available, it is recommended that glass, cans, and mixed paper be...

  7. 11. Photographic copy of construction drawing, dated July 29, 1932, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Photographic copy of construction drawing, dated July 29, 1932, Construction Division Office of the Quartermaster General, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. PLAN 625-2511 SDO 139 (FLOOR PLANS) - Selfridge Field, Building Nos. 246 & 253, 246 Birch Street & 253 Wagner Street, Mount Clemens, Macomb County, MI

  8. 32 CFR 246.5 - Responsibilities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...: (1) Provide policy and broad operational guidance to the Director of the AFIS. (2) Monitor and... the Unified Commands and the Director of the AFIS. 5 See footnote 1 to § 246.4(c). (3) Designate the... AFIS. (e) The Commander in Chief, U.S. European Command, and the Commander in Chief, U.S. Pacific...

  9. 32 CFR 246.5 - Responsibilities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...: (1) Provide policy and broad operational guidance to the Director of the AFIS. (2) Monitor and... the Unified Commands and the Director of the AFIS. 5 See footnote 1 to § 246.4(c). (3) Designate the... AFIS. (e) The Commander in Chief, U.S. European Command, and the Commander in Chief, U.S. Pacific...

  10. 48 CFR 52.246-9 - Inspection of Research and Development (Short Form).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Clauses 52.246-9 Inspection of Research and Development (Short Form). As prescribed in 46.309, insert the following clause: Inspection of Research and Development (Short Form) (APR 1984) The Government has the... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Inspection of Research and...

  11. Organobromine compound profiling in human adipose: Assessment of sources of bromophenol.

    PubMed

    Gao, Shixiong; Wan, Yi; Zheng, Guomao; Luo, Kai; Kannan, Kurunthachalam; Giesy, John P; Lam, Michael H W; Hu, Jianying

    2015-09-01

    Bromophenols (BRPs) have been widely detected in human tissues, however, relative proportions from natural products and/or anthropogenic flame retardants are not clear. 21 polybrominated diphenyl ethers (PBDEs), 15 MeO/OH-PBDEs, and 10 BRPs were simultaneously quantified in adipose collected from people from New York City, USA. An in vitro assay utilizing human liver microsomes was performed for detected predominant organobromine. High concentrations of 2,4,6-triBRP and PBDEs were observed, and extremely low concentrations of naturally occurring MeO/OH-PBDEs were detected. Similar biotransformatioin rates of BRPs and MeO/OH-PBDEs indicated that the relative high concentration of 2,4,6-triBRP in humans was not of natural origin. Significant correlation observed between concentrations of 2,4,6-triBRP and BDE-209 suggested that the two chemicals may share a common source. Both 2,4,6-triBRP and BDE-209 were detected in commercial ABS resins, suggesting that plastic products made from ABS resins could be potential sources of co-exposure of the two compounds for humans. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. Development of the small-molecule antiviral ST-246® as a smallpox therapeutic

    PubMed Central

    Grosenbach, Douglas W; Jordan, Robert; Hruby, Dennis E

    2011-01-01

    Naturally occurring smallpox has been eradicated, yet it remains as one of the highest priority pathogens due to its potential as a biological weapon. The majority of the US population would be vulnerable in a smallpox outbreak. SIGA Technologies, Inc. has responded to the call of the US government to develop and supply to the Strategic National Stockpile a smallpox antiviral to be deployed in the event of a smallpox outbreak. ST-246® (tecovirimat) was initially identified via a high-throughput screen in 2002, and in the ensuing years, our drug-development activities have spanned in vitro analysis, preclinical safety, pharmacokinetics and efficacy testing (all according to the ‘animal rule’). Additionally, SIGA has conducted Phase I and II clinical trials to evaluate the safety, tolerability and pharmacokinetics of ST-246, bringing us to our current late stage of clinical development. This article reviews the need for a smallpox therapeutic and our experience in developing ST-246, and provides perspective on the role of a smallpox antiviral during a smallpox public health emergency. PMID:21837250

  13. Tribromophenoxy flame retardants in the Great Lakes atmosphere.

    PubMed

    Ma, Yuning; Venier, Marta; Hites, Ronald A

    2012-12-18

    The 2,4,6-tribromophenoxy moiety is a common structural feature of several brominated flame retardants, and we have previously reported on the environmental concentrations of one such compound, 1,2-bis(2,4,6-tribromophenoxy) ethane (TBE). Here we report the atmospheric concentrations of TBE and three other tribromophenoxy compounds: allyl 2,4,6-tribromophenyl ether (ATE), 2-bromoallyl 2,4,6-tribromophenyl ether (BATE), and 2,3-dibromopropyl 2,4,6-tribromophenyl ether (DPTE). The samples were collected at five sites near the shores of the Great Lakes during the period 2008-2009, inclusive. Of these four compounds, TBE and ATE are currently used as flame retardants, and DPTE was formerly used as a flame retardant until its production ceased in the mid-1980s. The total concentrations of ATE, BATE, and DPTE were ∼2 pg/m³ in the cities of Chicago and Cleveland and 0.1-0.4 pg/m³ at the rural and remote sites. The concentrations of TBE were ∼1 pg/m³ in these cities and 0.2-0.8 pg/m³ at the rural and remote sites. In both cases, this was a very significant urban effect. The concentrations of ATE, BATE, and DPTE did not change significantly over the two-year study, but the concentrations of TBE decreased by about a factor of 2 during this time. This temporal change was statistically significant but not strong compared to the urban effect.

  14. In vitro efficacy of ST246 against smallpox and monkeypox.

    PubMed

    Smith, Scott K; Olson, Victoria A; Karem, Kevin L; Jordan, Robert; Hruby, Dennis E; Damon, Inger K

    2009-03-01

    Since the eradication of smallpox and the cessation of routine childhood vaccination for smallpox, the proportion of the world's population susceptible to infection with orthopoxviruses, such as variola virus (the causative agent of smallpox) and monkeypox virus, has grown substantially. In the United States, the only vaccines for smallpox licensed by the Food and Drug Administration (FDA) have been live virus vaccines. Unfortunately, a substantial number of people cannot receive live virus vaccines due to contraindications. Furthermore, no antiviral drugs have been fully approved by the FDA for the prevention or treatment of orthopoxvirus infection. Here, we show the inhibitory effect of one new antiviral compound, ST-246, on the in vitro growth properties of six variola virus strains and seven monkeypox virus strains. We performed multiple assays to monitor the cytopathic effect and to evaluate the reduction of viral progeny production and release in the presence of the compound. ST-246 had 50% effective concentrations of

  15. Lr41, Lr39, and a leaf rust resistance gene from Aegilops cylindrica may be allelic and are located on wheat chromosome 2DS.

    PubMed

    Singh, Sukhwinder; Franks, C D; Huang, L; Brown-Guedira, G L; Marshall, D S; Gill, B S; Fritz, A

    2004-02-01

    The leaf rust resistance gene Lr41 in wheat germplasm KS90WGRC10 and a resistance gene in wheat breeding line WX93D246-R-1 were transferred to Triticum aestivum from Aegilops tauschii and Ae. cylindrica, respectively. The leaf rust resistance gene in WX93D246-R-1 was located on wheat chromosome 2D by monosomic analysis. Molecular marker analysis of F(2) plants from non-critical crosses determined that this gene is 11.2 cM distal to marker Xgwm210 on the short arm of 2D. No susceptible plants were detected in a population of 300 F(2) plants from a cross between WX93D246-R-1 and TA 4186 ( Lr39), suggesting that the gene in WX93D246-R-1 is the same as, or closely linked to, Lr39. In addition, no susceptible plants were detected in a population of 180 F(2) plants from the cross between KS90WGRC10 and WX93D246-R-1. The resistance gene in KS90WGRC10, Lr41, was previously reported to be located on wheat chromosome 1D. In this study, no genetic association was found between Lr41 and 51 markers located on chromosome 1D. A population of 110 F(3 )lines from a cross between KS90WGRC10 and TAM 107 was evaluated with polymorphic SSR markers from chromosome 2D and marker Xgdm35 was found to be 1.9 cM proximal to Lr41. When evaluated with diverse isolates of Puccinia triticina, similar reactions were observed on WX93D246-R-1, KS90WGRC10, and TA 4186. The results of mapping, allelism, and race specificity test indicate that these germplasms likely have the same gene for resistance to leaf rust.

  16. Isolation from Estuarine Sediments of a Desulfovibrio Strain Which Can Grow on Lactate Coupled to the Reductive Dehalogenation of 2,4,6-Tribromophenol

    PubMed Central

    Boyle, Alfred W.; Phelps, Craig D.; Young, L. Y.

    1999-01-01

    Strain TBP-1, an anaerobic bacterium capable of reductively dehalogenating 2,4,6-tribromophenol to phenol, was isolated from estuarine sediments of the Arthur Kill in the New York/New Jersey harbor. It is a gram-negative, motile, vibrio-shaped, obligate anaerobe which grows on lactate, pyruvate, hydrogen, and fumarate when provided sulfate as an electron acceptor. The organism accumulates acetate when grown on lactate and sulfate, contains desulfoviridin, and will not grow in the absence of NaCl. It will not utilize acetate, succinate, propionate, or butyrate for growth via sulfate reduction. When supplied with lactate as an electron donor, strain TBP-1 will utilize sulfate, sulfite, sulfur, and thiosulfate for growth but not nitrate, fumarate, or acrylate. This organism debrominates 2-, 4-, 2,4-, 2,6-, and 2,4,6-bromophenol but not 3- or 2,3-bromophenol or monobrominated benzoates. It will not dehalogenate monochlorinated, fluorinated, or iodinated phenols or chlorinated benzoates. Together with its physiological characteristics, its 16S rRNA gene sequence places it in the genus Desulfovibrio. The average growth yield of strain TBP-1 grown on a defined medium supplemented with lactate and 2,4,6-bromophenol is 3.71 mg of protein/mmol of phenol produced, and the yield was 1.42 mg of protein/mmol of phenol produced when 4-bromophenol was the electron acceptor. Average growth yields (milligrams of protein per millimole of electrons utilized) for Desulfovibrio sp. strain TBP-1 grown with 2,4,6-bromophenol, 4-bromophenol, or sulfate are 0.62, 0.71, and 1.07, respectively. Growth did not occur when either lactate or 2,4,6-bromophenol was omitted from the growth medium. These results indicate that Desulfovibrio sp. strain TBP-1 is capable of growth via halorespiration. PMID:10049873

  17. Synthesis of fisetin and 2',4',6'-trihydroxydihyrochalcone 4'-O-β-neohesperidoside based on site-selective deacetylation and deoxygenation.

    PubMed

    Tsunekawa, Ryuji; Hanaya, Kengo; Higashibayashi, Shuhei; Sugai, Takeshi

    2018-04-26

    Fisetin and 2',4',6'-trihydroxydihyrochalcone 4'-O-β-neohesperidoside were synthesized from commercially available quercetin and naringin in five steps. The key steps are site-selective deacetylation and subsequent deoxygenation. The target molecules were obtained in 37% and 23% yields from the starting materials, respectively.

  18. TOXICITY OF TETRYL (N-METHYL-N,2,4,6-TETRANITROANILINE) IN F344 RATS

    EPA Science Inventory

    The toxicity of tetryl (N-methyl-N,2,4,6-tetranitroaniline) in male and female F344 rats was evaluated after adminstration in the diet for 14 or 90 days. The 14-day study diet concentrations used were 0, 500, 1250, 2000, 2500, and 5000 ppm; the 90-day study diet concentrations we...

  19. Web Page Development and Management. SPEC Kit 246 and SPEC Flyer 246.

    ERIC Educational Resources Information Center

    Liu, Yaping Peter, Comp.

    This SPEC (Systems and Procedures Exchange Center) Kit and Flyer reports results of two surveys conducted in 1996 and 1998 that examined ARL (Association of Research Libraries) member libraries' World Wide Web history, development, use, and activities. Fifty-six out of the then 119 ARL member institutions responded to the 1996 survey, and 68 out…

  20. New effective chemically synthesized anti-smallpox compound NIOCH-14.

    PubMed

    Mazurkov, Oleg Yu; Kabanov, Alexey S; Shishkina, Larisa N; Sergeev, Alexander A; Skarnovich, Maksim O; Bormotov, Nikolay I; Skarnovich, Maria A; Ovchinnikova, Alena S; Titova, Ksenya A; Galahova, Darya O; Bulychev, Leonid E; Sergeev, Artemiy A; Taranov, Oleg S; Selivanov, Boris A; Tikhonov, Alexey Ya; Zavjalov, Evgenii L; Agafonov, Alexander P; Sergeev, Alexander N

    2016-05-01

    Antiviral activity of the new chemically synthesized compound NIOCH-14 (a derivative of tricyclodicarboxylic acid) in comparison with ST-246 (the condensed derivative of pyrroledione) was observed in experiments in vitro and in vivo using orthopoxviruses including highly pathogenic ones. After oral administration of NIOCH-14 to outbred ICR mice infected intranasally with 100 % lethal dose of ectromelia virus, it was shown that 50 % effective doses of NIOCH-14 and ST-246 did not significantly differ. The 'therapeutic window' varied from 1 day before infection to 6 days post-infection (p.i.) to achieve 100-60 % survival rate. The administration of NIOCH-14 and ST-246 to mice resulted in a significant reduction of ectromelia virus titres in organs examined as compared with the control and also reduced pathological changes in the lungs 6 days p.i. Oral administration of NIOCH-14 and ST-246 to ICR mice and marmots challenged with monkeypox virus as compared with the control resulted in a significant reduction of virus production in the lungs and the proportion of infected mice 7 days p.i. as well as the absence of disease in marmots. Significantly lower proportions of infected mice and virus production levels in the lungs as compared with the control were demonstrated in experiments after oral administration of NIOCH-14 and ST-246 to ICR mice and immunodeficient SCID mice challenged with variola virus 3 and 4 days p.i., respectively. The results obtained suggest good prospects for further study of the chemical compound NIOCH-14 to create a new smallpox drug on its basis.

  1. Disulfide isomerase-like protein AtPDIL1–2 is a good candidate for trichlorophenol phytodetoxification

    PubMed Central

    Peng, Ri-He; Qiu, Jin; Tian, Yong-Sheng; Gao, Jian-jie; Han, Hong-juan; Fu, Xiao-Yan; Zhu, Bo; Xu, Jing; Wang, Bo; Li, Zhen-jun; Wang, Li-juan; Yao, Quan-Hong

    2017-01-01

    Trichlorophenol (TCP) is a widely used and persistent environmentally toxic compound that poses a carcinogenic risk to humans. Phytoremediation is a proficient cleanup technology for organic pollutants. In this study, we found that the disulfide isomerase-like protein AtPDIL1–2 in plants is a good candidate for enhancing 2,4,6-TCP phytoremediation. The expression of AtPDIL1-2 in Arabidopsis was induced by 2,4,6-TCP. The heterologously expressed AtPDIL1-2 in Escherichia coli exhibited both oxidase and isomerase activities as protein disulfide isomerase and improved bacteria tolerance to 2,4,6-TCP. Further research revealed that transgenic tobacco overexpressing AtPDIL1-2 was more tolerant to high concentrations of 2,4,6-TCP and removed the toxic compound at far greater rates than the control plants. To elucidate the mechanism of action of AtPDIL1-2, we investigated the chemical interaction of AtPDIL1-2 with 2,4,6-TCP for the first time. HPLC analysis implied that AtPDIL1-2 exerts a TCP-binding activity. A suitable configuration of AtPDIL1-2-TCP binding was obtained by molecular docking studies using the AutoDock program. It predicted that the TCP binding site is located in the b-b′ domain of AtPDIL1-2 and that His254 of the protein is critical for the binding interaction. These findings imply that AtPDIL1-2 can be used for TCP detoxification by the way of overexpression in plants. PMID:28059139

  2. 78 FR 13549 - Special Supplemental Nutrition Program for Women, Infants and Children (WIC): Implementation of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... FI must usually be purchased in totality. In addition, FIs do not yield data on the type, amount, and... amend Sec. 246.2 to add the definition of EBT. Cash-Value Voucher/Cash-Value Benefit. Federal WIC regulations (Sec. 246.2) provide definitions for cash-value vouchers (CVV) and food instruments, and within...

  3. Preparation of 1,3,5-triamo-2,4,6-trinitrobenzene of submicron particle size

    DOEpatents

    Rigdon, Lester P [Livermore, CA; Moody, Gordon L [Tracy, CA; McGuire, Raymond R [Brentwood, CA

    2001-05-01

    A method is disclosed for the preparation of very small particle size, relatively pure 1,3,5-triamino-2,4,6-trinitrobenzene (TATB). Particles of TATB prepared according to the disclosed method are of submicron size and have a surface area in the range from about 3.8 to 27 square meters per gram.

  4. BIOTRANSFORMATION OF 2,4,6-TRINITROTOLUENE IN A CONTINUOUS-FLOW ANABAENA SP. SYSTEM. (R825513C013)

    EPA Science Inventory

    Reductive transformation of 2,4,6-trinitrotoluene (TNT) was observed in a continuous-flow system of Anabaena sp. operated for 33 d with a 5.7 d hydraulic retention time and a range of influent TNT concentrations of 1–58 mg/l. The TNT removal effici...

  5. Watermelon germplasm lines USVL246-FR2 and USVL252-FR2 tolerant to fusarium oxysporum f. sp. niveum race 2

    USDA-ARS?s Scientific Manuscript database

    Two improved germplasm lines of wild watermelon (Citrullus lanatus var. citroides) designated USVL246-FR2 and USVL252-FR2 were released in 2012 by the Agricultural Research Service of the U.S. Department of Agriculture (Wechter et al. 2012). These lines are each highly uniform for growth characteri...

  6. Preparation of 1,3,5-triamino-2,4,6-trinitrobenzene of submicron particle size

    DOEpatents

    Rigdon, Lester P.; Moody, Gordon L.; McGuire, Raymond R.

    2001-01-01

    A method is disclosed for the preparation of very small particle size, relatively pure 1,3,5-triamino-2,4,6-trinitrobenzene (TATB). Particles of TATB prepared according to the disclosed method are of submicron size and have a surface area in the range from about 3.8 to 27 square meters per gram.

  7. Photolysis of 2,4,6-trinitrotoluene in seawater and estuary water: Impact of pH, temperature, salinity, and dissolved organic matter.

    PubMed

    Luning Prak, Dianne J; Breuer, James E T; Rios, Evelyn A; Jedlicka, Erin E; O'Sullivan, Daniel W

    2017-01-30

    The influence of salinity, pH, temperature, and dissolved organic matter on the photolysis rate of 2,4,6-trinitrotoluene (TNT) in marine, estuary, and laboratory-prepared waters was studied using a Suntest CPS+® solar simulator equipped with optical filters. TNT degradation rates were determined using HPLC analysis, and products were identified using LC/MS. Minimal or no TNT photolysis occurred under a 395-nm long pass filter, but under a 295-nm filter, first-order TNT degradation rate constants and apparent quantum yields increased with increasing salinity in both natural and artificial seawater. TNT rate constants increased slightly with increasing temperature (10 to 32°C) but did not change significantly with pH (6.4 to 8.1). The addition of dissolved organic matter (up to 5mg/L) to ultrapure water, artificial seawater, and natural seawater increased the TNT photolysis rate constant. Products formed by TNT photolysis in natural seawater were determined to be 2,4,6-trinitrobenzaldehyde, 1,3,5-trinitrobenzene, 2,4,6-trinitrobenzoic acid, and 2-amino-4,6-dinitrobenzoic acid. Published by Elsevier Ltd.

  8. Structural properties, vibrational spectra and surface-enhanced Raman scattering of 2,4,6-trichloro- and tribromoanilines: A comparative study

    NASA Astrophysics Data System (ADS)

    Haruna, Kabiru; Saleh, Tawfik A.; Al Thagfi, Jameel; Al-Saadi, Abdulaziz A.

    2016-10-01

    A comparative electronic and spectroscopic analysis of 2,4,6-trichloroaniline (TCA) and 2,4,6-tribromoaniline (TBA) was carried out by theoretical and experimental techniques. The NH2 inversion barrier in TCA and TBA molecules was predicted to be three times less than that in aniline and 2,4,6-trifluoroaniline. The size of the halogen substituents in the ortho positions is shown by density functional theory to play an important role in determining the electronic and structural properties of the amino group in the investigated haloaniline derivatives. A thorough interpretation of the infrared and Raman spectra has been performed on the basis of the observed and calculated infrared and Raman spectra as well as calculated potential energy distribution values. In addition, the SERS spectra for both trihaloanilines were successfully collected up to a concentration of 10-6 M using aged hydroxylamine-reduced silver colloid as an active substrate for TCA and TBA. SERS intensities of several peaks were found to linearly change with concentration allowing quantitative analyses of TCA and TBA. A relatively stronger interaction in the case of TBA-silver colloids is predicted compared to the TCA analogue.

  9. Preparation of a N-Heterocyclic Carbene Nickel(II) Complex: Synthetic Experiments in Current Organic and Organometallic Chemistry

    ERIC Educational Resources Information Center

    Ritleng, Vincent; Brenner, Eric; Chetcuti, Michael J.

    2008-01-01

    A four-part experiment that leads to the synthesis of a cyclopentadienyl chloro-nickel(II) complex bearing a N-heterocyclic carbene (NHC) ligand is presented. In the first part, the preparation of 1,3-bis-(2,4,6-trimethylphenyl)imidazolium chloride (IMes[middle dot]HCl) in a one-pot procedure by reaction of 2,4,6-trimethylaniline with…

  10. Highly crystalline covalent organic frameworks from flexible building blocks.

    PubMed

    Xu, Liqian; Ding, San-Yuan; Liu, Junmin; Sun, Junliang; Wang, Wei; Zheng, Qi-Yu

    2016-03-28

    Two novel 2D covalent organic frameworks (TPT-COF-1 and TPT-COF-2) were synthesized from the flexible 2,4,6-triaryloxy-1,3,5-triazine building blocks on a gram scale, which show high crystallinity and large surface area. The controllable formation of highly ordered frameworks is mainly attributed to the self-assembly Piedfort unit of 2,4,6-triaryloxy-1,3,5-triazine.

  11. Nonhuman Primates are Protected from Smallpox Virus or Monkeypox Virus Challenges by the Antiviral Drug ST-246

    DTIC Science & Technology

    2009-06-01

    Drug ST-246 John Huggins,1 Arthur Goff,1 Lisa Hensley,1 Eric Mucker,1 Josh Shamblin,1 Carly Wlazlowski,1 Wendy Johnson,1 Jennifer Chapman,1 Tom Larsen...Hauer, M. Layton , J. McDade, M. T. Osterholm, T. O’Toole, G. Parker, T. Perl, P. K. Russell, K. Tonat, and the Working Group on Civilian Biodefense

  12. A combination of p53-activating APR-246 and phosphatidylserine-targeting antibody potently inhibits tumor development in hormone-dependent mutant p53-expressing breast cancer xenografts

    PubMed Central

    Liang, Yayun; Mafuvadze, Benford; Besch-Williford, Cynthia; Hyder, Salman M

    2018-01-01

    Background Between 30 and 40% of human breast cancers express a defective tumor suppressor p53 gene. Wild-type p53 tumor suppressor protein promotes cell-cycle arrest and apoptosis and inhibits vascular endothelial growth factor–dependent angiogenesis, whereas mutant p53 protein (mtp53) lacks these functions, resulting in tumor cell survival and metastasis. Restoration of p53 function is therefore a promising drug-targeted strategy for combating mtp53-expressing breast cancer. Methods In this study, we sought to determine whether administration of APR-246, a small-molecule drug that restores p53 function, in combination with 2aG4, an antibody that targets phosphatidylserine residues on tumor blood vessels and disrupts tumor vasculature, effectively inhibits advanced hormone-dependent breast cancer tumor growth. Results APR-246 reduced cell viability in mtp53-expressing BT-474 and T47-D human breast cancer cells in vitro, and significantly induced apoptosis in a dose-dependent manner. However, APR-246 did not reduce cell viability in MCF-7 breast cancer cells, which express wild-type p53. We next examined APR-246’s anti-tumor effects in vivo using BT-474 and T47-D tumor xenografts established in female nude mice. Tumor-bearing mice were treated with APR-246 and/or 2aG4 and tumor volume followed over time. Tumor growth was more effectively suppressed by combination treatment than by either agent alone, and combination therapy completely eradicated some tumors. Immunohistochemistry analysis of tumor tissue sections demonstrated that combination therapy more effectively induced apoptosis and reduced cell proliferation in tumor xenografts than either agent alone. Importantly, combination therapy dramatically reduced the density of blood vessels, which serve as the major route for tumor metastasis, in tumor xenografts compared with either agent alone. Conclusion Based on our findings, we contend that breast tumor growth might effectively be controlled by simultaneous targeting of mtp53 protein and tumor blood vessels in mtp53-expressing cancers. PMID:29606888

  13. [Evaluation of therapeutic-prophylactic effectiveness of chemical compound NIOC-14 against ectromelia virus in vivo].

    PubMed

    Kabanov, A S; Shishkina, L N; Mazurkov, O Iu; Skarnovich, M O; Bormotov, N I; Serova, O A; Sergeev, Al A; Sergeev, Ar A; Selivanov, B A; Tikhonov, A Iu; Agafonov, A P; Sergeev, A N

    2015-01-01

    Study pharmacodynamic parameters of anti-viral effectiveness of a chemical compound NIOC-14 in experiments in mice infected with ectromelia virus (EV). EV (K-1 strain) was obtained from the State Collection of Viral Infections and Rickettsioses Causative Agents of the State Scientific Centre of Virology and Biotechnology "Vector". Outbred ICR mice were intranasally infected with EV at a dose of 10 LD50 per animal (10 x 50% lethal doses/animal) and per orally received NIOC-14 or ST-246 as a positive control. Chemical compound NIOC-14 (7-[N'-(4-trifluoromethylbenzoyl)-hidrazincarbonyl]-tricyclo[3.2.2.0(2,4)]non-8-en-6-carbonic acid) was synthesized in Novosibirsk Institute of Organic Chemistry (NIOC). Anti-pox preparation ST-246, developed by SIGA Technologies Inc. (USA), was synthesized in NIOC using the technique described by the authors. 50% effective doses against EV in vivo were shown not to differ significantly between the preparations NIOC-14 (3.59 μg/g mouse mass) and ST-246 (5.08 μg/g mouse mass). During determination of therapeutic window, administration of NIOC-14 to mice 1 day or 1 hour before EV infection, as well as 1, 2 and 4 days after EV infection and then for 9 days was found to ensure 100% animal survival. Administration of NIOC-14 as well as ST-246 resulted in the decrease relative to control of EV titers in lungs, nasal cavity, brains, liver, spleen, kidneys and pancreas. Anti-viral effectiveness of NIOC-14 against EV in vivo was thus comparable by all the studied pharmacodynamic parameters with anti-viral activity of anti-pox-virus preparation ST-246.

  14. Application of Novel Amino-Functionalized NZVI@SiO2 Nanoparticles to Enhance Anaerobic Granular Sludge Removal of 2,4,6-Trichlorophenol.

    PubMed

    Guan, Zeyu; Wan, Jinquan; Ma, Yongwen; Wang, Yan; Shu, Yajie

    2015-01-01

    A novel amino-functionalized silica-coated nanoscale zerovalent iron (NZVI@SiO2-NH2) was successfully synthesized by using one-step liquid-phase method with the surface functionalization of nanoscale zerovalent iron (NZVI) to enhance degradation of chlorinated organic contaminants from anaerobic microbial system. NZVI@SiO2-NH2 nanoparticles were synthesized under optimal conditions with the uniform core-shell structure (80-100 nm), high loading of amino functionality (~0.9 wt%), and relatively large specific surface area (126.3 m(2)/g). The result demonstrated that well-dispersed NZVI@SiO2-NH2 nanoparticle with nFe(0)-core and amino-functional silicon shell can effectively remove 2,4,6-trichlorophenol (2,4,6-TCP) in the neutral condition, much higher than that of NZVI. Besides, the surface-modified nanoparticles (NZVI@SiO2-NH2) in anaerobic granule sludge system also showed a positive effect to promote anaerobic biodechlorination system. More than 94.6% of 2,4,6-TCP was removed from the combined NZVI@SiO2-NH2-anaerobic granular sludge system during the anaerobic dechlorination processes. Moreover, adding the appropriate concentration of NZVI@SiO2-NH2 in anaerobic granular sludge treatment system can decrease the toxicity of 2,4,6-TCP to anaerobic microorganisms and improved the cumulative amount of methane production and electron transport system activity. The results from this study clearly demonstrated that the NZVI@SiO2-NH2/anaerobic granular sludge system could become an effective and promising technology for the removal of chlorophenols in industrial wastewater.

  15. Alternative Flame Retardant, 2,4,6-Tris(2,4,6-tribromophenoxy)-1,3,5-triazine, in an E-waste Recycling Facility and House Dust in North America.

    PubMed

    Guo, Jiehong; Stubbings, William A; Romanak, Kevin; Nguyen, Linh V; Jantunen, Liisa; Melymuk, Lisa; Arrandale, Victoria; Diamond, Miriam L; Venier, Marta

    2018-03-20

    A high molecular weight compound, 2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine (TTBP-TAZ), was detected during the analysis of brominated flame retardants in dust samples collected from an electrical and electronic waste (e-waste) recycling facility in Ontario, Canada. Gas chromatography coupled with both high-resolution and low-resolution mass spectrometry (MS) was used to determine TTBP-TAZ's chemical structure and concentrations. To date, TTBP-TAZ has only been detected in plastic casings of electrical and electronic equipment and house dust from The Netherlands. Here we report on the concentrations of TTBP-TAZ in selected samples from North America: e-waste dust ( n = 7) and air ( n = 4), residential dust ( n = 30), and selected outdoor air ( n = 146), precipitation ( n = 19), sediment ( n = 11) and water ( n = 2) samples from the Great Lakes environment. TTBP-TAZ was detected in all the e-waste dust and air samples, and in 70% of residential dust samples. The median concentrations of TTBP-TAZ in these three types of samples were 5540 ng/g, 5.75 ng/m 3 and 6.76 ng/g, respectively. The flame retardants 2,4,6-tribromophenol, tris(2,3-dibromopropyl) isocyanurate, and 3,3',5,5'-tetrabromobisphenol A bis(2,3-dibromopropyl) ether, BDE-47 and BDE-209 were also measured for comparison. None of these other flame retardants concentrations was significantly correlated with those of TTBP-TAZ in any of the sample types suggesting different sources. TTBP-TAZ was not detected in any of the outdoor environmental samples, which may relate to its application history and physicochemical properties. This is the first report of TTBP-TAZ in North America.

  16. Single turnover studies of oxidative halophenol dehalogenation by horseradish peroxidase reveal a mechanism involving two consecutive one electron steps: toward a functional halophenol bioremediation catalyst.

    PubMed

    Sumithran, Suganya; Sono, Masanori; Raner, Gregory M; Dawson, John H

    2012-12-01

    Horseradish peroxidase (HRP) catalyzes the oxidative para-dechlorination of the environmental pollutant/carcinogen 2,4,6-trichlorophenol (2,4,6-TCP). A possible mechanism for this reaction is a direct oxygen atom transfer from HRP compound I (HRP I) to trichlorophenol to generate 2,6-dichloro 1,4-benzoquinone, a two-electron transfer process. An alternative mechanism involves two consecutive one-electron transfer steps in which HRP I is reduced to compound II (HRP II) and then to the ferric enzyme as first proposed by Wiese et al. [F.W. Wiese, H.C. Chang, R.V. Lloyd, J.P. Freeman, V.M. Samokyszyn, Arch. Environ. Contam. Toxicol. 34 (1998) 217-222]. To probe the mechanism of oxidative halophenol dehalogenation, the reactions between 2,4,6-TCP and HRP compounds I or II have been investigated under single turnover conditions (i.e., without excess H(2)O(2)) using rapid scan stopped-flow spectroscopy. Addition of 2,4,6-TCP to HRP I leads rapidly to HRP II and then more slowly to the ferric resting state, consistent with a mechanism involving two consecutive one-electron oxidations of the substrate via a phenoxy radical intermediate. HRP II can also directly dechlorinate 2,4,6-TCP as judged by rapid scan stopped-flow and mass spectrometry. This observation is particularly significant since HRP II can only carry out one-electron oxidations. A more detailed understanding of the mechanism of oxidative halophenol dehalogenation will facilitate the use of HRP as a halophenol bioremediation catalyst. Copyright © 2012 Elsevier Inc. All rights reserved.

  17. Application of Novel Amino-Functionalized NZVI@SiO2 Nanoparticles to Enhance Anaerobic Granular Sludge Removal of 2,4,6-Trichlorophenol

    PubMed Central

    Guan, Zeyu; Wan, Jinquan; Ma, Yongwen; Wang, Yan; Shu, Yajie

    2015-01-01

    A novel amino-functionalized silica-coated nanoscale zerovalent iron (NZVI@SiO2-NH2) was successfully synthesized by using one-step liquid-phase method with the surface functionalization of nanoscale zerovalent iron (NZVI) to enhance degradation of chlorinated organic contaminants from anaerobic microbial system. NZVI@SiO2-NH2 nanoparticles were synthesized under optimal conditions with the uniform core-shell structure (80–100 nm), high loading of amino functionality (~0.9 wt%), and relatively large specific surface area (126.3 m2/g). The result demonstrated that well-dispersed NZVI@SiO2-NH2 nanoparticle with nFe0-core and amino-functional silicon shell can effectively remove 2,4,6-trichlorophenol (2,4,6-TCP) in the neutral condition, much higher than that of NZVI. Besides, the surface-modified nanoparticles (NZVI@SiO2-NH2) in anaerobic granule sludge system also showed a positive effect to promote anaerobic biodechlorination system. More than 94.6% of 2,4,6-TCP was removed from the combined NZVI@SiO2-NH2-anaerobic granular sludge system during the anaerobic dechlorination processes. Moreover, adding the appropriate concentration of NZVI@SiO2-NH2 in anaerobic granular sludge treatment system can decrease the toxicity of 2,4,6-TCP to anaerobic microorganisms and improved the cumulative amount of methane production and electron transport system activity. The results from this study clearly demonstrated that the NZVI@SiO2-NH2/anaerobic granular sludge system could become an effective and promising technology for the removal of chlorophenols in industrial wastewater. PMID:26060427

  18. Protein-protein interactions and substrate channeling in orthologous and chimeric aldolase-dehydrogenase complexes.

    PubMed

    Baker, Perrin; Hillis, Colleen; Carere, Jason; Seah, Stephen Y K

    2012-03-06

    Bacterial aldolase-dehydrogenase complexes catalyze the last steps in the meta cleavage pathway of aromatic hydrocarbon degradation. The aldolase (TTHB246) and dehydrogenase (TTHB247) from Thermus thermophilus were separately expressed and purified from recombinant Escherichia coli. The aldolase forms a dimer, while the dehydrogenase is a monomer; these enzymes can form a stable tetrameric complex in vitro, consisting of two aldolase and two dehydrogenase subunits. Upon complex formation, the K(m) value of 4-hydroxy-2-oxopentanoate, the substrate of TTHB246, is decreased 4-fold while the K(m) of acetaldehyde, the substrate of TTHB247, is increased 3-fold. The k(cat) values of each enzyme were reduced by ~2-fold when they were in a complex. The half-life of TTHB247 at 50 °C increased by ~4-fold when it was in a complex with TTHB246. The acetaldehyde product from TTHB246 could be efficiently channelled directly to TTHB247, but the channeling efficiency for the larger propionaldehyde was ~40% lower. A single A324G substitution in TTHB246 increased the channeling efficiency of propionaldehyde to a value comparable to that of acetaldehyde. Stable and catalytically competent chimeric complexes could be formed between the T. thermophilus enzymes and the orthologous aldolase (BphI) and dehydrogenase (BphJ) from the biphenyl degradation pathway of Burkholderia xenovorans LB400. However, channeling efficiencies for acetaldehyde in these chimeric complexes were ~10%. Structural and sequence analysis suggests that interacting residues in the interface of the aldolase-dehydrogenase complex are highly conserved among homologues, but coevolution of partner enzymes is required to fine-tune this interaction to allow for efficient substrate channeling.

  19. Methylation of bacterial release factors RF1 and RF2 is required for normal translation termination in vivo.

    PubMed

    Mora, Liliana; Heurgué-Hamard, Valérie; de Zamaroczy, Miklos; Kervestin, Stephanie; Buckingham, Richard H

    2007-12-07

    Bacterial release factors RF1 and RF2 are methylated on the Gln residue of a universally conserved tripeptide motif GGQ, which interacts with the peptidyl transferase center of the large ribosomal subunit, triggering hydrolysis of the ester bond in peptidyl-tRNA and releasing the newly synthesized polypeptide from the ribosome. In vitro experiments have shown that the activity of RF2 is stimulated by Gln methylation. The viability of Escherichia coli K12 strains depends on the integrity of the release factor methyltransferase PrmC, because K12 strains are partially deficient in RF2 activity due to the presence of a Thr residue at position 246 instead of Ala. Here, we study in vivo RF1 and RF2 activity at termination codons in competition with programmed frameshifting and the effect of the Ala-246 --> Thr mutation. PrmC inactivation reduces the specific termination activity of RF1 and RF2(Ala-246) by approximately 3- to 4-fold. The mutation Ala-246 --> Thr in RF2 reduces the termination activity in cells approximately 5-fold. After correction for the decrease in level of RF2 due to the autocontrol of RF2 synthesis, the mutation Ala-246 --> Thr reduced RF2 termination activity by approximately 10-fold at UGA codons and UAA codons. PrmC inactivation had no effect on cell growth in rich media but reduced growth considerably on poor carbon sources. This suggests that the expression of some genes needed for optimal growth under such conditions can become growth limiting as a result of inefficient translation termination.

  20. Contamination trends and factors affecting the transfer of hexabromocyclododecane diastereomers, tetrabromobisphenol A, and 2,4,6-tribromophenol to breast milk in Japan.

    PubMed

    Fujii, Yukiko; Kato, Yoshihisa; Masuda, Nanako; Harada, Kouji H; Koizumi, Akio; Haraguchi, Koichi

    2018-06-01

    This study investigated contamination trends and factors affecting the levels of brominated flame retardants (BFRs), including hexabromocyclododecane (HBCD) diastereomers, tetrabromobisphenol A (TBBP-A), and 2,4,6-tribromophenol (2,4,6-TBP), in breast milk in Japan. Breast milk samples (n = 64) were collected from mothers living in six prefectures in Japan. The mean concentrations were 2.2, 0.19, 0.29, 3.0, and 0.59 ng/g lipid weight for α-HBCD, β-HBCD, γ-HBCD, TBBP-A, and 2,4,6-TBP, respectively. Based on the previous studies, the levels of ΣHBCD in Japanese women's milk appear to be increasing, and the levels of TBBP-A are higher than those in other Asian countries. Although ΣHBCD were not correlated to phenolic BFRs, the concentration of β-HBCD was significantly correlated to the concentrations of TBBP-A (r = 0.440, p < 0.01) and 2,4,6-TBP (r = 0.320, p < 0.01). The concentration of γ-HBCD increased significantly with maternal age (r = 0.378, p < 0.01), but the concentrations of the other analytes were not dependent on age. The concentration of α-HBCD was higher in primiparae than in multiparae (p < 0.05), while TBBP-A was higher in multiparae. No significant correlation was found between the phenolic BFR levels in milk and mothers' age, working place, and drinking/smoking habits. These results suggest that exposure to α- and γ-HBCD diastereomers could be affected by maternal age and parity, respectively, because of their different kinetics and sources. Therefore, these factors should be considered when conducting infant risk assessments. Copyright © 2018. Published by Elsevier Ltd.

  1. Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from Arabidopsis

    PubMed Central

    Guo, Yongxia; Zheng, Zuyu; La Clair, James J.; Chory, Joanne; Noel, Joseph P.

    2013-01-01

    Genetic studies in Arabidopsis implicate an α/β-hydrolase, KARRIKIN-INSENSITIVE 2 (KAI2) as a receptor for karrikins, germination-promoting butenolide small molecules found in the smoke of burned plants. However, direct biochemical evidence for the interaction between KAI2 and karrikin and for the mechanism of downstream signaling by a KAI2–karrikin complex remain elusive. We report crystallographic analyses and ligand-binding experiments for KAI2 recognition of karrikins. The karrikin-1 (KAR1) ligand sits in the opening to the active site abutting a helical domain insert but distal from the canonical catalytic triad (Ser95-His246-Asp217) of α/β-hydrolases, consistent with the lack of detectable hydrolytic activity by purified KAI2. The closest approach of KAR1 to Ser95-His246-Asp217 is 3.8 Å from His246. Six aromatic side chains, including His246, encapsulate KAR1 through geometrically defined aromatic–aromatic interactions. KAR1 binding induces a conformational change in KAI2 at the active site entrance. A crevice of hydrophobic residues linking the polar edge of KAR1 and the helical domain insert suggests that KAI2–KAR1 creates a contiguous interface for binding signaling partners in a ligand-dependent manner. PMID:23613584

  2. Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from Arabidopsis.

    PubMed

    Guo, Yongxia; Zheng, Zuyu; La Clair, James J; Chory, Joanne; Noel, Joseph P

    2013-05-14

    Genetic studies in Arabidopsis implicate an α/β-hydrolase, KARRIKIN-INSENSITIVE 2 (KAI2) as a receptor for karrikins, germination-promoting butenolide small molecules found in the smoke of burned plants. However, direct biochemical evidence for the interaction between KAI2 and karrikin and for the mechanism of downstream signaling by a KAI2-karrikin complex remain elusive. We report crystallographic analyses and ligand-binding experiments for KAI2 recognition of karrikins. The karrikin-1 (KAR1) ligand sits in the opening to the active site abutting a helical domain insert but distal from the canonical catalytic triad (Ser95-His246-Asp217) of α/β-hydrolases, consistent with the lack of detectable hydrolytic activity by purified KAI2. The closest approach of KAR1 to Ser95-His246-Asp217 is 3.8 Å from His246. Six aromatic side chains, including His246, encapsulate KAR1 through geometrically defined aromatic-aromatic interactions. KAR1 binding induces a conformational change in KAI2 at the active site entrance. A crevice of hydrophobic residues linking the polar edge of KAR1 and the helical domain insert suggests that KAI2-KAR1 creates a contiguous interface for binding signaling partners in a ligand-dependent manner.

  3. Hemin immobilized into metal-organic frameworks as an electrochemical biosensor for 2,4,6-trichlorophenol

    NASA Astrophysics Data System (ADS)

    Zhang, Ting; Wang, Lu; Gao, Congwei; Zhao, Chaoyue; Wang, Yang; Wang, Jianmin

    2018-02-01

    Hemin immobilized into copper-based metal-organic frameworks was successfully prepared and used as a new electrode material for sensitive electrochemical biosensing. X-ray diffraction patterns, Fourier transform infrared spectra, scanning electron microscopy, UV-vis absorption spectroscopy, and cyclic voltammetry were used to characterize the resultant composites. Due to the interaction between the copper atom groups and hemin, the constrained environment in Cu-MOF-74 acts as a matrix to avoid the dimerization of enzyme molecules and retain its biological activity. The hemin/Cu-MOF composites demonstrated enhanced electrocatalytical activity and high stability towards the oxidation of 2,4,6-trichlorophenol. Under optimum experimental conditions, the sensor showed a wide linear relationship over the range of 0.01-9 μmol L-1 with a detection limit (3σ) of 0.005 μmol L-1. The relative standard deviations were 4.6% and 3.5% for five repeated measurements of 0.5 and 5 μmol L-1 2,4,6-trichlorophenol, respectively. The detection platforms for 2,4,6-trichlorophenol developed here not only indicate that hemin/Cu-MOF-74 possesses intrinsic biological reactivity, but also enable further work to be conducted towards the application of enzyme-containing metal-organic frameworks in electrochemical biosensors.

  4. Stress responses to trichlorophenol in Arabidopsis and integrative analysis of alteration in transcriptional profiling from microarray.

    PubMed

    Li, Zhenjun; Zhu, Bo; Wang, Bo; Gao, Jianjie; Fu, Xiaoyan; Yao, Quanhong

    2015-01-25

    Trichlorophenols, also known as TCPs, are one of the most persistent environmental pollutants. It is a matter of concern as they are toxic and cumulative in soil and water bodies which could lead to serious consequences to the biosphere. How plants respond to this compound has rarely been examined previously. In our study, detailed morphological and physiological responses of Arabidopsis to 2,4,6-trichlorophenol, a representative TCP, were investigated. Seed germination and seedling growth were markedly inhibited by 2,4,6-TCP. Furthermore, we performed gene expression profiling analysis upon 2,4,6-TCP treatment in Arabidopsis and identified 34 transcripts induced and 212 repressed more than four folds. Gene Ontology (GO) analysis showed that these TCP-responsive genes are involved in various biological processes, such as secondary metabolism, biological regulation, response to stimulus and other processes related to growth and development. The activities of two reactive oxygen species-related enzymes (peroxidase and superoxide dismutase) and the content of malondialdehyde were increased significantly after 2,4,6-TCP treatment. Our findings have the potential to provide valuable gene resources and theoretical information for more in-depth analyses of TCPs' response in Arabidopsis thaliana and even other organic pollutants. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Hemin immobilized into metal-organic frameworks as an electrochemical biosensor for 2,4,6-trichlorophenol.

    PubMed

    Zhang, Ting; Wang, Lu; Gao, Congwei; Zhao, Chaoyue; Wang, Yang; Wang, Jianmin

    2018-02-16

    Hemin immobilized into copper-based metal-organic frameworks was successfully prepared and used as a new electrode material for sensitive electrochemical biosensing. X-ray diffraction patterns, Fourier transform infrared spectra, scanning electron microscopy, UV-vis absorption spectroscopy, and cyclic voltammetry were used to characterize the resultant composites. Due to the interaction between the copper atom groups and hemin, the constrained environment in Cu-MOF-74 acts as a matrix to avoid the dimerization of enzyme molecules and retain its biological activity. The hemin/Cu-MOF composites demonstrated enhanced electrocatalytical activity and high stability towards the oxidation of 2,4,6-trichlorophenol. Under optimum experimental conditions, the sensor showed a wide linear relationship over the range of 0.01-9 μmol L -1 with a detection limit (3σ) of 0.005 μmol L -1 . The relative standard deviations were 4.6% and 3.5% for five repeated measurements of 0.5 and 5 μmol L -1 2,4,6-trichlorophenol, respectively. The detection platforms for 2,4,6-trichlorophenol developed here not only indicate that hemin/Cu-MOF-74 possesses intrinsic biological reactivity, but also enable further work to be conducted towards the application of enzyme-containing metal-organic frameworks in electrochemical biosensors.

  6. 40 CFR 721.5356 - Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl]-omega...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Ethanol, 2,2â²2â³-nitrilotris... Substances § 721.5356 Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl... subject to reporting. (1) The chemical substance identified as ethanol, 2,2′2″-nitrilotris-, compound with...

  7. 40 CFR 721.5356 - Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl]-omega...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Ethanol, 2,2â²2â³-nitrilotris... Substances § 721.5356 Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl... subject to reporting. (1) The chemical substance identified as ethanol, 2,2′2″-nitrilotris-, compound with...

  8. 40 CFR 721.5356 - Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl]-omega...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Ethanol, 2,2â²2â³-nitrilotris... Substances § 721.5356 Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl... subject to reporting. (1) The chemical substance identified as ethanol, 2,2′2″-nitrilotris-, compound with...

  9. 40 CFR 721.5356 - Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl]-omega...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Ethanol, 2,2â²2â³-nitrilotris... Substances § 721.5356 Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl... subject to reporting. (1) The chemical substance identified as ethanol, 2,2′2″-nitrilotris-, compound with...

  10. 40 CFR 721.5356 - Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl]-omega...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Ethanol, 2,2â²2â³-nitrilotris... Substances § 721.5356 Ethanol, 2,2′2″-nitrilotris-, compound with alpha-2,4,6-tris (1-phenylethyl)phenyl... subject to reporting. (1) The chemical substance identified as ethanol, 2,2′2″-nitrilotris-, compound with...

  11. A POC Clinical Trial for PTSD with a First-In-Class Vasopressin 1a Receptor Antagonist

    DTIC Science & Technology

    2016-10-01

    benefit of SRX246 for the treatment of anger , irritability, and aggression; major depression ; disturbed sleep; and quality of life that frequently...treatment of major depression , anger , irritability, and aggression, disturbed sleep, and quality of life that frequently accompany PTSD. In this...BID) and ii) clinical benefit of SRX246 for the treatment of major depression ; anger , irritability, and aggression; disturbed sleep; and quality of

  12. Metabolic Engineering of Plants to Produce Precursors (Phloroglucinol and 1,2,4-butanetriol) of Energetic Materials

    DTIC Science & Technology

    2015-01-02

    phloroglucinol, which are precursors of energetic materials butanetriol trinitrate (BTTN) and l ,3,5-triamino-2,4,6 trinitrobenzene (TATB), respectively, in...of energetic materials butanetriol trinitrate (BTTN) and l ,3,5-triamino-2,4,6 trinitrobenzene (TATB), respectively, in plants. The strategy was to... phenylalanine , valine and hexose sugars. On the other hand the metabolites that are completely depleted in the chloroplastic lines and partially in

  13. Biological treatment of TNT-contaminated soil. 1: Anaerobic cometabolic reduction and interaction of TNT and metabolites with soil components

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Daun, G.; Lenke, H.; Knackmuss, H.J.

    1998-07-01

    The explosive 2,4,6-trinitrotoluene (TNT), found as a major contaminant at armament plants from the two world wars, is reduced by a variety of microorganisms when electron donors such as glucose are added. This study shows that the cometabolic reduction of TNT to 2,4,6-triaminotoluene by an undefined anaerobic consortium increased considerably with increasing TNT concentrations and decreased with decreasing concentrations and feeding rates of glucose. The interactions of TNT and its reduction products with montmorillonitic clay and humic acids were investigated in abiotic adsorption experiments and during the microbial reduction of TNT. The results indicate that reduction products of TNT particularlymore » hydroxylaminodinitrotoluenes and 2,4,6-triaminotoluene bind irreversibly to soil components, which would prevent or prolong mineralization of the contaminants. Irreversible binding also hinders a further spread of the contaminants through soil or leaching into the groundwater.« less

  14. Degradation of 2,4,6-trinitrotoluene by immobilized horseradish peroxidase and electrogenerated peroxide.

    PubMed

    Beom Lee, Ki; Bock Gu, Man; Moon, Seung Hyeon

    2003-03-01

    This paper presents horseradish peroxidase (HRP)-catalyzed removal of 2,4,6-trinitrotoluene (TNT) by an electrochemical packed-bed flow reactor operated in a circulating batch mode with the help of in situ generated hydrogen peroxide. HRP immobilized on the reticulated vitreous carbon electrode was prepared for the cyclic voltammetry of 2,4,6-TNT. Effects of pH and temperature on the TNT electroreduction in 0.2M phosphate buffer saturated with oxygen were examined. HRP immobilized carbon electrode was capable of catalyzing the oxidation and detoxification of 44 microM TNT in aqueous solution under optimized conditions. The removal rate of TNT for the electroenzymatic method was much greater than for electrochemical and biochemical methods. Stoichiometric and kinetic studies indicated that the hydrogen peroxide was utilized more effectively in the electroenzymatic method. Denitrification as intermediate reaction was also investigated.

  15. Information and Communication Technology Use Among Low-Income Pregnant and Postpartum Women by Race and Ethnicity: A Cross-Sectional Study.

    PubMed

    Chilukuri, Nymisha; West, Meredith; Henderson, Janice Lynn; Lawson, Shari; Ehsanipoor, Robert; Costigan, Kathleen; Polk, Sarah; Bennett, Wendy

    2015-07-03

    Pregnancy and the postpartum period provide windows of opportunity to impact perinatal and lifelong preventive health behavior for women and their families, but these opportunities are often missed. Understanding racial/ethnic differences in information and communication technology (ICT) use could inform technology-based interventions in diverse populations. The objective of the study was to evaluate differences in the use of ICT between racial and ethnic groups as well as by English language proficiency. We conducted a cross-sectional study of 246 women who were aged 18 years or older and pregnant or within 1 year of delivery. They were recruited from 4 hospital-based outpatient clinics and completed a self-administered survey. We used multivariate regression analysis to evaluate the association between race/ethnicity and ICT (mobile phone/short message service [SMS] text message, Internet, and social network) usage by race/ethnicity and perceived English language proficiency after adjusting for age, income, marital status, and insurance status. In all, 28% (69/246) of participants were Latina, 40% (98/246) were African American, 23% (56/246) were white, and 9% (23/246) from other racial/ethnic groups. Of the Latinas, 84% (58/69) reported limited English language proficiency and 59% (41/69) were uninsured. More than 90% of all participants reported mobile phone use, but more than 25% (65/246) had changed phone numbers 2 or more times in the past year. Compared to white women, African American women were less likely to SMS text message (OR 0.07, 95% CI 0.01-0.63) and Latinas were less likely to use the Internet to find others with similar concerns (OR 0.23, 95% CI 0.08-0.73). Women with limited English language proficiency were less likely to use the Internet overall (OR 0.30, 95% CI 0.09-0.99) or use email (OR 0.22, 95% CI 0.08-0.63) compared to women with adequate English language proficiency. Mobile phones are widely available for the delivery of health interventions to low-income, racially diverse pregnant and postpartum women, but disparities in Internet use and SMS text messaging exist. Interventions or programs requiring Web-based apps may have lower uptake unless alternatives are available, such as those adapted for limited English proficiency populations.

  16. Draft Genome Sequence of Bacillus sp. GZT, a 2,4,6-Tribromophenol-Degrading Strain Isolated from the River Sludge of an Electronic Waste-Dismantling Region

    PubMed Central

    Liang, Zhishu; Li, Guiying; Das, Ranjit

    2016-01-01

    Here, we report the draft genome sequence of Bacillus sp. strain GZT, a 2,4,6-tribromophenol (TBP)-degrading bacterium previously isolated from an electronic waste-dismantling region. The draft genome sequence is 5.18 Mb and has a G+C content of 35.1%. This is the first genome report of a brominated flame retardant-degrading strain. PMID:27257197

  17. Machine Learning Intermolecular Potentials for 1,3,5-Triamino-2,4,6-trinitrobenzene (TATB) Using Symmetry-Adapted Perturbation Theory

    DTIC Science & Technology

    2018-04-25

    unlimited. NOTICES Disclaimers The findings in this report are not to be construed as an official Department of the Army position unless so...this report, intermolecular potentials for 1,3,5-triamino-2,4,6-trinitrobenzene (TATB) are developed using machine learning techniques. Three...potentials based on support vector regression, kernel ridge regression, and a neural network are fit using symmetry-adapted perturbation theory. The

  18. Degradation of TATP, TNT, and RDX using mechanically alloyed metals

    NASA Technical Reports Server (NTRS)

    Geiger, Cherie (Inventor); Sigman, Michael (Inventor); Fidler, Rebecca (Inventor); Clausen, Christian (Inventor)

    2012-01-01

    Bimetallic alloys prepared in a ball milling process, such as iron nickel (FeNi), iron palladium (FePd), and magnesium palladium (MgPd) provide in situ catalyst system for remediating and degrading nitro explosive compounds. Specifically, munitions, such as, 2,4,6-trinitrotoluene (TNT), cyclo-1,3,5-trimethylene-2,4,6-trinitramine (RDX), nitrocellulose and nitroglycerine that have become contaminants in groundwater, soil, and other structures are treated on site to remediate explosive contamination.

  19. Navy Nuclear-Powered Surface Ships: Background, Issues, and Options for Congress

    DTIC Science & Technology

    2010-09-29

    to design a smaller scale version of a naval pressurized water reactor , or to design a new reactor type potentially using a thorium liquid salt...integrated nuclear power system capable of use on destroyer- sized vessels either using a pressurized water reactor or a thorium liquid salt reactor ...nuclear reactors for Navy surface ships. The text of Section 246 is as follows: SEC. 246. STUDY ON THORIUM -LIQUID FUELED REACTORS FOR NAVAL FORCES

  20. Navy Nuclear-Powered Surface Ships: Background, Issues, and Options for Congress

    DTIC Science & Technology

    2010-03-29

    246 of H.R. 2647 would require DOD to submit to the congressional defense committees a study on the use of thorium -liquid fueled nuclear reactors ...Congressional Research Service 19 SEC. 246. STUDY ON THORIUM -LIQUID FUELED REACTORS FOR NAVAL FORCES. (a) Study Required- The Secretary of Defense and...the Chairman of the Joint Chiefs of Staff shall jointly carry out a study on the use of thorium -liquid fueled nuclear reactors for naval power

  1. Analysis of blaCTX-M-Carrying Plasmids from Escherichia coli Isolates Collected in the BfT-GermVet Study ▿

    PubMed Central

    Schink, Anne-Kathrin; Kadlec, Kristina; Schwarz, Stefan

    2011-01-01

    In this study, 417 Escherichia coli isolates from defined disease conditions of companion and farm animals collected in the BfT-GermVet study were investigated for the presence of extended-spectrum β-lactamase (ESBL) genes. Three ESBL-producing E. coli isolates were identified among the 100 ampicillin-resistant isolates. The E. coli isolates 168 and 246, of canine and porcine origins, respectively, harbored blaCTX-M-1, and the canine isolate 913 harbored blaCTX-M-15, as confirmed by PCR and sequence analysis. The isolates 168 and 246 belonged to the novel multilocus sequence typing (MLST) types ST1576 and ST1153, respectively, while isolate 913 had the MLST type ST410. The ESBL genes were located on structurally related IncN plasmids in isolates 168 and 246 and on an IncF plasmid in isolate 913. The blaCTX-M-1 upstream regions of plasmids pCTX168 and pCTX246 were similar, whereas the downstream regions showed structural differences. The genetic environment of the blaCTX-M-15 gene on plasmid pCTX913 differed distinctly from that of both blaCTX-M-1 genes. Detailed sequence analysis showed that the integration of insertion sequences, as well as interplasmid recombination events, accounted for the structural variability in the blaCTX-M gene regions. PMID:21685166

  2. Vicarious nucleophilic substitution to prepare 1,3-diamino-2,4,6-trinitrobenzene or 1,3,5-triamino-2,4,6-trinitrobenzene

    DOEpatents

    Mitchell, A.R.; Pagoria, P.F.; Schmidt, R.D.

    1996-10-29

    The present invention relates to a process to produce 1,3-diamino-2,4,6-trinitrobenzene (DATB) or 1,3,5-triamino-2,4,6,trinitrobenzene (TATB) by: (a) reacting at ambient pressure and a temperature of between about 0 and 50 C for between about 0.1 and 24 hr, a trinitroaromatic compound of the structure shown within where X, Y, and Z are each independently selected from --H, or --NH{sub 2}, with the proviso that at least 1 or 2 of X, Y, and Z are hydrogen, with an amount effective to produce DATB or TATB, or 1,1,1-trialkylhydrazinium halide wherein alkyl is selected from methyl, ethyl, propyl or butyl and halide is selected from chloride, bromide or iodide, in the presence of a strong base selected from sodium butoxide, potassium butoxide, potassium propoxide, sodium propoxide, sodium ethoxide, potassium ethoxide, sodium methoxide, potassium methoxide, and combinations thereof; in a solvent selected from the group consisting of methanol, ethanol, propanol, butanol, dimethylsulfoxide, N-methylpyrrolidone, hexamethylphosphoramide, dimethylformide, dimethylacetamide and mixtures thereof, provided that when alcohols are present primarily DATB and picramide is formed; and (b) isolating the DATB or TATB produced. DATB and TATB are useful specialty explosives. TATB is also used for the preparation of benzenehexamine, a starting material for the synthesis of novel materials (optical imaging devices, liquid crystals, ferromagnetic compounds).

  3. Vicarious nucleophilic substitution using 4-amino-1,2,4-triazole, hydroxylamine or O-alkylhydroxylamine to prepare 1,3-diamino-2,4,6-trinitrobenzene or 1,3,5-triamino-2,4,6-trinitrobenzene

    DOEpatents

    Mitchell, A.R.; Pagoria, P.F.; Schmidt, R.D.

    1997-05-27

    The present invention relates to a process to produce 1,3-diamino-2,4,6-trinitrobenzene (DATB) or 1,3,5-triamino-2,4,6,trinitrobenzene (TATB) by: (a) reacting at ambient pressure and a temperature of between about 0 and 50 C for between about 0.1 and 24 hr, a trinitroaromatic compound of the structure shown where X, Y, and Z are each independently selected from the group consisting of -H and -NH{sub 2}, with the proviso that at least 1 or 2 of X, Y, and Z are hydrogen; with an effective amount of 1-amino-1,2,4-triazole, hydroxylamine or O-alkylhydroxamine to produce DATB or TATB; in the presence of a strong base selected from sodium butoxide, potassium butoxide, potassium propoxide, sodium propoxide, sodium ethoxide, potassium ethoxide, sodium methoxide, potassium methoxide, and combinations thereof; in a solvent selected from the group consisting of methanol, ethanol, propanol, butanol, dimethylsulphoxide, N-methylpyrrolidone, hexamethylphosphoramide, dimethylformide, dimethylacetamide and mixtures thereof, provided that when alcohols are present or when hydroxylamine or its O-alkyl derivatives replace ATA primarily DATB is formed; and (b) isolating the DATB or TATB produced. DATB and TATB are important and useful specialty explosives and intermediates for other materials.

  4. Vicarious nucleophilic substitution using 4-amino-1,2,4-triazole, hydroxylamine or O-alkylhydroxylamine to prepare 1,3-diamino-2,4,6-trinitrobenzene or 1,3,5-triamino-2,4,6-trinitrobenzene

    DOEpatents

    Mitchell, Alexander R.; Pagoria, Philip F.; Schmidt, Robert D.

    1997-01-01

    The present invention relates to a process to produce 1,3-diamino-2,4,6-trinitrobenzene (DATB) or 1,3,5-triamino-2,4,6,-trinitrobenzene (TATB) by: (a) reacting at ambient pressure and a temperature of between about 0.degree. and 50.degree. C. for between about 0.1 and 24 hr, a trinitroaromatic compound of structure V: ##STR1## wherein X, Y, and Z are each independently selected from the group consisting of --H and --NH.sub.2, with the proviso that at least 1 or 2 of X, Y, and Z are hydrogen; with an effective amount of 1-amino-1,2,4-triazole, hydroxylamine or O-alkylhydroxamine to produce DATB or TATB; in the presence of a strong base selected from sodium butoxide, potassium butoxide, potassium propoxide, sodium propoxide, sodium ethoxide, potassium ethoxide, sodium methoxide, potassium methoxide, and combinations thereof; in a solvent selected from the group consisting of methanol, ethanol, propanol, butanol, dimethylsulphoxide, N-methylpyrrolidone, hexamethylphosphoramide, dimethylformide, dimethylacetamide and mixtures thereof, provided that when alcohols are present or when hydroxylamine or its O-alkyl derivatives replace ATA primarily DATB is formed; and (b) isolating the DATB or TATB produced. DATB and TATB are important and useful specialty explosives and intermediates for other materials.

  5. Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Finlay, C.A.; Hinds, P.W.; Tan, T.H.

    1988-02-01

    The 11-4 p53 cDNA clone failed to transform primary rat fibroblasts when cotransfected with the ras oncogene. Two linker insertion mutations at amino acid 158 or 215 (of 390 amino acids) activated this p53 cDNA for transformation with ras. These mutant cDNAs produced a p53 protein that lacked an epitope, recognized by monoclonal antibody PAb246 (localized at amino acids 88 to 110 in the protein) and preferentially bound to a heat shock protein, hsc70. In rat cells transformed by a genomic p53 clone plus ras, two populations of p53 proteins were detected, PAb246/sup +/ and PAb246/sup -/, which did ormore » did not bind to this monoclonal antibody, respectively. The PAb246/sup -/ p53 preferentially associated with hsc70, and this protein has a half-life 4- to 20-fold longer than free p53 (PAb246/sup +/). These data suggest a possible functional role for hsc70 in the transformation process. cDNAs for p53 derived from methylcholanthrene-transformed cells transform rat cells in cooperation with the ras oncogene and produce a protein that bound with the heat shock proteins. Recombinant clones produced between a Meth A cDNA and 11-4 were tested for the ability to transform rat cells. A single amino acid substitution at residue 132 was sufficient to activate the 11-4 p53 cDNA for transformation. These studies have identified a region between amino acids 132 and 215 in the p53 protein which, when mutated, can activate the p53 cDNA. These results also call into question what the correct p53 wild-type sequence is and whether a wild-type p53 gene can transform cells in culture.« less

  6. a Computational Tddft Study on Intramolecular Charge Transfer in Di-Tert and 2,4,6-TRICYANOANILINES.

    NASA Astrophysics Data System (ADS)

    Fujiwara, Takashige; Zgierski, Marek Z.

    2014-06-01

    We have carried out TDDFT computational studies on the low-lying excited states of di-tert-butylaminobenzonitrile and 2,4,6-tricyanoaniline compounds that exhibit unusual photophysical behaviors associated with the intramolecular charge transfer (ICT). For both 3- and 4-di-tert-butylamino)benzonitriles (m-DTBABN and p-DTBABN, respectively) show the ICT formation, and p-DTBABN appears to be the only meta-substituted aminobenzonitrile that exhibits the ICT formation. The TDDFT calculations indicate evidence that the ultrafast ICT formation in p-DTBABN and m-DTBABN is due to the sequential state switches: ππ*(La)→ πσ*→ ICT in the presence of conical intersections among the three closely-lying excited-states. On the other hand, 2,4,6-tricyanoaniline does not show clear evidence for the LE (locally excited) state → ICT state formation from steady-state fluorescence studies, despite the greater electron acceptor strength of tricycanobenzene as compared to monocyanobenzene, which is part of a 4-(dimethylamino)benzonitrile (p-DMABN) compound. However, it is predicted that 2,4,6-tricyano-N,N-dimethylaniline (TCDMA), but not 2,4,6-tricyanoaniline (TCA), possesses two ICT states, which show the ICT-characterized quinoidal structures and lie below the initially photo-excited S1(ππ*) state. The CC2 calculations further predict two conformers as labeled with quinoidal (ICT--Q) and anti-quinoidal (ICT--AQ) structures are rapidly interconnecting with each other. The lower energy ICT--Q structure tends to be populated from the unstable ICT--AQ structure, which is responsible for the observed time-resolved fluorescence as well as the excited-state absorption from the mixed S1(ππ*)/ICT state of TCDMA. In both cases for TCDMA and TCA, the πσ* state locates significantly higher in energy than the S1(ππ*) state (and the ICT state for TCA), thus precluding the πσ*→ ICT formation, which is believed to occur in a p-DMABN in polar environments.

  7. Aerobic Growth of Escherichia coli with 2,4,6-Trinitrotoluene (TNT) as the Sole Nitrogen Source and Evidence of TNT Denitration by Whole Cells and Cell-Free Extracts▿ †

    PubMed Central

    Stenuit, Ben; Eyers, Laurent; Rozenberg, Raoul; Habib-Jiwan, Jean-Louis; Agathos, Spiros N.

    2006-01-01

    Escherichia coli grew aerobically with 2,4,6-trinitrotoluene (TNT) as sole nitrogen source and caused TNT's partial denitration. This reaction was enhanced in nongrowing cell suspensions with 0.516 mol nitrite released per mol TNT. Cell extracts denitrated TNT in the presence of NAD(P)H. Isomers of amino-dimethyl-tetranitrobiphenyl were detected and confirmed with U-15N-labeled TNT. PMID:17012591

  8. Proceedings of the Annual Meeting of the American Society of Tropical Medicine and Hygiene (37th) Held in Washington, D.C. on December 4-8, 1988

    DTIC Science & Technology

    1988-12-30

    Diseases, Fort Detrick, Frederick, MD. 246 PREDICTION OF MALARIA TRANSMISSION POTENTIAL IN CHIAPAS , MEXICO THROUGH USE OF NASA REMOTE-SENSING...field surveys. PREDICTION OF MALARIA TRANSMISSION POTENTIAL IN CHIAPAS , MEXICO 246 THROUGH USE OF NASA REMOTE-SENSING TECHNOLOGY D. Strickman, D... Tuberculosis . F. Gordin, P. Hopewell, F. Luelmo, H. Rieder* and G. Slutkin WEDNESDAY, DECEMBER 7 9:00 AM - 11:30 AM PLENARY SESSION ON PRIORITIES IN

  9. Spot test for 1,3,5-triamino-2,4,6-trinitrobenzene, TATB

    DOEpatents

    Harris, Betty W.

    1986-01-01

    A simple, sensitive and specific spot test for 1,3,5-triamino-2,4,6-trinitrobenzene, TATB, is described. Upon the application of the composition of matter of the present invention to samples containing in excess of 0.1 mg of this explosive, a bright orange color results. Interfering species such as TNT and Tetryl can be removed by first treating the sample with a solvent which does not dissolve much of the TATB, but readily dissolves these explosives.

  10. Spot test for 1,3,5-triamino-2,4,6-trinitrobenzene, TATB

    DOEpatents

    Harris, B.W.

    1984-11-29

    A simple, sensitive and specific spot test for 1,3,5-triamino-2,4,6-trinitrobenzene, TATB, is described. Upon the application of the composition of matter of the subject invention to samples containing in excess of 0.1 mg of this explosive, a bright orange color results. Interfering species such as TNT and Tetryl can be removed by first treating the sample with a solvent which does not dissolve the TATB, but readily dissolves these interfering explosives.

  11. Fiber-Reinforced Superalloys For Rocket Engines

    NASA Technical Reports Server (NTRS)

    Lewis, Jack R.; Yuen, Jim L.; Petrasek, Donald W.; Stephens, Joseph R.

    1990-01-01

    Report discusses experimental studies of fiber-reinforced superalloy (FRS) composite materials for use in turbine blades in rocket engines. Intended to withstand extreme conditions of high temperature, thermal shock, atmospheres containing hydrogen, high cycle fatigue loading, and thermal fatigue, which tax capabilities of even most-advanced current blade material - directionally-solidified, hafnium-modified MAR M-246 {MAR M-246 (Hf) (DS)}. FRS composites attractive combination of properties for use in turbopump blades of advanced rocket engines at temperatures from 870 to 1,100 degrees C.

  12. Isomer effects on polyimide properties

    NASA Technical Reports Server (NTRS)

    Stump, B. L.

    1975-01-01

    Polyimide polymers which are thermally stable and processable are developed. The addition of alkyl substituents to an aromatic ring in the polymer backbone is examined along with polyimide precursor amines containing functional groups that allow for post-cure crosslinking. The synthesis of key monomers is reported, including 2,4,6-tris (m-aminobenzyl) 1,3,5-trimethyl benzene and 2,4,6-tris (p-aminobenzyl) 1,3,5-trimethyl benzene. The preparation of a key monomer, 2,5,3-triamino benzophenone, is reported.

  13. On the Non-Metallicity of 2.2 nm Au 246 (SR) 80 Nanoclusters

    DOE PAGES

    Zhou, Meng; Zeng, Chenjie; Song, Yongbo; ...

    2017-11-22

    The transition from molecular to plasmonic behaviour in metal nanoparticles with increasing size remains a central question in nanoscience. Here, we report that the giant 246-gold-atom nanocluster (2.2 nm in gold core diameter) protected by 80 thiolate ligands is surprisingly non-metallic based on UV/Vis and femtosecond transient absorption spectroscopy as well as electrochemical measurements. Specifically, the Au246 nanocluster exhibits multiple excitonic peaks in transient absorption spectra and electron dynamics independent of the pump power, which are in contrast to the behaviour of metallic gold nanoparticles. Moreover, a prominent oscillatory feature with frequency of 0.5 THz can be observed in almostmore » all the probe wavelengths. The phase and amplitude analysis of the oscillation suggests that it arises from the wavepacket motion on the ground state potential energy surface, which also indicates the presence of a small band-gap and thus non-metallic or molecular-like behaviour.« less

  14. Identification of hydrophobic amino acids required for lipid activation of C. elegans CTP:phosphocholine cytidylyltransferase.

    PubMed

    Braker, Jay D; Hodel, Kevin J; Mullins, David R; Friesen, Jon A

    2009-12-01

    CTP:phosphocholine cytidylyltransferase (CCT), critical for phosphatidylcholine biosynthesis, is activated by translocation to the membrane surface. The lipid activation region of Caenorhabditis elegans CCT is between residues 246 and 266 of the 347 amino acid polypeptide, a region proposed to form an amphipathic alpha helix. When leucine 246, tryptophan 249, isoleucine 256, isoleucine 257, or phenylalanine 260, on the hydrophobic face of the helix, were changed individually to serine low activity was observed in the absence of lipid vesicles, similar to wild-type CCT, while lipid stimulated activity was reduced compared to wild-type CCT. Mutational analysis of phenylalanine 260 implicated this residue as a contributor to auto-inhibition of CCT while mutation of L246, W249, I256, and I257 simultaneously to serine resulted in significantly higher activity in the absence of lipid vesicles and an enzyme that was not lipid activated. These results support a concerted mechanism of lipid activation that requires multiple residues on the hydrophobic face of the putative amphipathic alpha helix.

  15. Pharmacokinetic and pharmacodynamic modeling to determine the dose of ST-246 to protect against smallpox in humans.

    PubMed

    Leeds, Janet M; Fenneteau, Frederique; Gosselin, Nathalie H; Mouksassi, Mohamad-Samer; Kassir, Nastya; Marier, J F; Chen, Yali; Grosenbach, Doug; Frimm, Annie E; Honeychurch, Kady M; Chinsangaram, Jarasvech; Tyavanagimatt, Shanthakumar R; Hruby, Dennis E; Jordan, Robert

    2013-03-01

    Although smallpox has been eradicated, the United States government considers it a "material threat" and has funded the discovery and development of potential therapeutic compounds. As reported here, the human efficacious dose for one of these compounds, ST-246, was determined using efficacy studies in nonhuman primates (NHPs), together with pharmacokinetic and pharmacodynamic analysis that predicted the appropriate dose and exposure levels to provide therapeutic benefit in humans. The efficacy analysis combined the data from studies conducted at three separate facilities that evaluated treatment following infection with a closely related virus, monkeypox virus (MPXV), in a total of 96 NHPs. The effect of infection on ST-246 pharmacokinetics in NHPs was applied to humans using population pharmacokinetic models. Exposure at the selected human dose of 600 mg is more than 4-fold higher than the lowest efficacious dose in NHPs and is predicted to provide protection to more than 95% of the population.

  16. Transformation among Aromatic Iodinated Disinfection Byproducts in the Presence of Monochloramine: From Monoiodophenol to Triiodophenol and Diiodonitrophenol.

    PubMed

    Gong, Tingting; Tao, Yuxian; Zhang, Xiangru; Hu, Shaoyang; Yin, Jinbao; Xian, Qiming; Ma, Jian; Xu, Bin

    2017-09-19

    Aromatic iodinated disinfection byproducts (DBPs) are a newly identified category of highly toxic DBPs. Among the identified aromatic iodinated DBPs, 2,4,6-triiodophenol and 2,6-diiodo-4-nitrophenol have shown relatively widespread occurrence and high toxicity. In this study, we found that 4-iodophenol underwent transformation to form 2,4,6-triiodophenol and 2,6-diiodo-4-nitrophenol in the presence of monochloramine. The transformation pathways were investigated, the decomposition kinetics of 4-iodophenol and the formation of 2,4,6-triiodophenol and 2,6-diiodo-4-nitrophenol were studied, the factors affecting the transformation were examined, the toxicity change during the transformation was evaluated, and the occurrence of the proposed transformation pathways during chloramination of source water was verified. The results revealed that 2,4,6-triiodophenol and 2,6-diiodo-4-nitrophenol, which could account for 71.0% of iodine in the transformed 4-iodophenol, were important iodinated transformation products of 4-iodophenol in the presence of monochloramine. The transformation pathways of 4-iodophenol in the presence of monochloramine were proposed and verified. The decomposition of 4-iodophenol in the presence of monochloramine followed a pseudo-second-order decay. Various factors including monochloramine dose, pH, temperature, nitrite concentration, and free chlorine contact time (before chloramination) affected the transformation. The cytotoxicity of the chloraminated 4-iodophenol samples increased continuously with contact time. The proposed transformation pathways occurred during chloramination of source water.

  17. Pharmacokinetic Comparison of a Single Oral Dose of Polymorph Form I versus Form V Capsules of the Antiorthopoxvirus Compound ST-246 in Human Volunteers

    PubMed Central

    Chinsangaram, Jarasvech; Honeychurch, Kady M.; Tyavanagimatt, Shanthakumar R.; Bolken, Tove' C.; Jordan, Robert; Jones, Kevin F.; Marbury, Thomas; Lichtenstein, Israel; Pickens, Margaret; Corrado, Michael; Landis, Patrick; Clarke, Jean M.; Frimm, Annie M.

    2012-01-01

    ST-246, a novel compound that inhibits egress of orthopoxvirus from mammalian cells, is being tested as a treatment for pathogenic orthopoxvirus infections in humans. This phase I, double-blind, randomized, crossover, exploratory study was conducted to compare the pharmacokinetics (PK) of a single daily 400-mg oral dose of ST-246 polymorph form I versus polymorph form V administered to fed, healthy human volunteers. Both forms appeared to be well tolerated, with no serious adverse events. The order of administration of the two forms had no effect on the results of the PK analyses. Form I and form V both exhibited comparable plasma concentration versus time profiles, but complete bioequivalence between the two forms was not found. Maximum drug concentration (Cmax) met the bioequivalence criteria, as the 90% confidence interval (CI) was 80.6 to 96.9%. However, the area under the concentration-time curve from time zero to time t (AUC0-t) and AUC0-∞ did not meet the bioequivalence criteria (CIs of 67.8 to 91.0% and 73.9 to 104.7%, respectively). The extent of absorption of form I, as defined by AUC0-∞, was 11.7% lower than that of form V. Since ST-246 form I is more thermostable than form V, form I was selected for further development and use in all future studies. PMID:22526314

  18. Vicarious nucleophilic substitution to prepare 1,3-diamino-2,4,6-trinitrobenzene or 1,3,5-triamino-2,4,6-trinitrobenzene

    DOEpatents

    Mitchell, Alexander R.; Pagoria, Philip F.; Schmidt, Robert D.

    1996-01-01

    The present invention relates to a process to produce 1,3-diamino-2,4,6-trinitrobenzene (DATB) or 1,3,5-triamino-2,4,6,-trinitrobenzene (TATB) by: (a) reacting at ambient pressure and a temperature of between about 0.degree. and 50.degree. C. for between about 0.1 and 24 hr, a trinitroaromatic compound of structure V: ##STR1## wherein X, Y, and Z are each independently selected from --H, or --NH.sub.2, with the proviso that at least 1 or 2 of X, Y, and Z are hydrogen, with an amount effective to produce DATB or TATB of 1,1,1-trialkylhydrazinium halide wherein alkyl is selected from methyl, ethyl, propyl or butyl and halide is selected from chloride, bromide or iodide. in the presence of a strong base selected from sodium butoxide, potassium butoxide, potassium propoxide, sodium propoxide, sodium ethoxide, potassium ethoxide, sodium methoxide, potassium methoxide, and combinations thereof; in a solvent selected from the group consisting of methanol, ethanol, propanol, butanol, dimethylsulphoxide, N-methylpyrrolidone, hexamethylphosphoramide, dimethylformide, dimethylacetamide and mixtures thereof, provided that when alcohols are present primarily DATB and picramide is formed; and (b) isolating the DATB or TATB produced. DATB and TATB are useful specialty explosives. TATB is also used for the preparation of benzenehexamine, a starting material for the synthesis of novel materials (optical imaging devices, liquid crystals, ferromagnetic compounds).

  19. Transformation of 2,4,6-trinitrotoluene (TNT) by actinomycetes isolated from TNT-contaminated and uncontaminated environments.

    PubMed Central

    Pasti-Grigsby, M B; Lewis, T A; Crawford, D L; Crawford, R L

    1996-01-01

    Actinomycete strains isolated from 2,4,6-trinitrotoluene (TNT)-contaminated and uncontaminated environments were compared for TNT tolerance and abilities to transform TNT. Regardless of previous TNT exposure history, no significant differences in TNT tolerance were seen among strains. Selected strains did not significantly mineralize [14C]TNT. The actinomycetes did, however, transform TNT into reduced intermediates. The data indicate that, in actinomycete-rich aerobic environments like composts, actinomycetes will transform TNT into intermediates which are known to form recalcitrant polymers. PMID:8975606

  20. Human Cell Line Activation Test of the Novel Energetics 2,6-pyrazinediamine 3,5-dinitro 1 -oxide (LLM-105) and 2,4,6-trinitro-3-bromoanisole (TNBA)

    DTIC Science & Technology

    2017-11-06

    substances early in the RDT&E process to avoid unnecessary costs, conserve physical resources, and sustain the health of those potentially exposed. The SERDP...pyrazinediamine 3,5-dinitro 1 –oxide (LLM-105) and 2,4,6-trinitro-3-bromoanisole (TNBA) Prepared by: Emily Reinke, Ph.D. Health Effects Division...Toxicology Directorate Army Public Health Center ARIMS designation: 500c Use of trademarked name(s) does not imply endorsement by the U.S

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