75 FR 51478 - Notice of Availability of the Draft Supplemental Environmental Impact Statement for the Cortez...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-20

... amended, the Bureau of Land Management (BLM), Battle Mountain District, Mount Lewis Field Office, Battle Mountain, Nevada, has prepared a Draft Supplemental Environmental Impact Statement (EIS) for the Cortez... meetings in Crescent Valley and Battle Mountain, Nevada. The BLM will announce future meetings or hearings...

75 FR 41516 - Notice of Intent To Prepare a Supplemental Environmental Impact Statement for the Cortez Hills...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-16

..., the Bureau of Land Management (BLM), Battle Mountain District, Mount Lewis Field Office, Battle Mountain, Nevada, intends to prepare a Supplemental Environmental Impact Statement (EIS) for the Cortez... Cortez Hills Expansion Project are available at the BLM Battle Mountain District Office, 50 Bastian Road...

77 FR 55224 - Notice of Availability of the Proposed Imperial Sand Dunes Recreation Area Management Plan and...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-07

...: Regular Mail: BLM Director (210), Attention: Brenda Hudgens- Williams, P.O. Box 71383, Washington, DC 20024-1383. Overnight Mail: BLM Director (210), Attention: Brenda Hudgens- Williams, 20 M Street SE...; and visual resources. The Proposed Imperial Sand Dunes RAMP and CDCA Plan Amendment/Final EIS includes...

76 FR 44000 - Environmental Impact Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-22

..., NV, Salt Wells Energy Projects, Proposal for Three Separate Geothermal Energy and Transmission...-328-4200. EIS No. 20110233, Draft EIS, BLM, WY, Chokecherry and Sierra Madre Wind Energy Project, Proposes to Construct and Operate a Wind Energy Project, South of Rawlins, Carbon County, WY, Comment...

78 FR 40474 - Environmental Impact Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-05

..., Draft EIS, USA, TX, Implementation of Energy, Water, and Solid Waste Sustainability Initiatives at Fort... Land Management (BLM) has adopted the Federal Energy Regulatory Commission's FEIS 20100001, filed 01/07.... 20130197, Final EIS, USACE, FL, Everglades Agricultural Area A- 1 Shallow Flow Equalization Basin, Review...

76 FR 2705 - Notice of Availability of the Final Supplemental Environmental Impact Statement for the Cortez...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-14

... amended, the Bureau of Land Management (BLM), Battle Mountain District, Mount Lewis Field Office, Battle Mountain, Nevada, has prepared a Final Supplemental Environmental Impact Statement (EIS) for the Cortez... Final Supplemental EIS are available for public inspection at the Battle Mountain District Office, 50...

Evaluation of EIS alternatives by the science integration team, volume I.

Treesearch

Thomas M. Quigley; Kristine M. Lee; Sylvia J. Arbelbide

1997-01-01

The Evaluation of EIS Alternatives by the Science Integration Team describes the outcomes, interactions, effects, and consequences likely to result from implementing seven different management strategies on Forest Service (FS) and Bureau of Land Management (BLM) administered lands within the Interior Columbia Basin and portions of the Klamath and Great Basins. Two...

75 FR 13275 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

... Dimmett 208-382- 7400. EIS No. 20100083, Draft EIS, BLM, NV, Amargosa Farm Road Solar Energy Project, Construction and Operation of Two Concentrated Solar Power Plant Facilities, Right-of-Way Application on Public..., Blythe Solar Power Project (09- AFC-6), Application for Right-of Way Grant to Construct and Operate, and...

77 FR 44267 - Notice of Availability of the Final Programmatic Environmental Impact Statement for Solar Energy...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-27

... Final EIS NOA and use them to inform the Records of Decision. Those individuals wishing to submit... general information regarding the DOE NEPA process, contact Carol Borgstrom, Director, Office of NEPA... development. Through the Final Programmatic EIS, the BLM is considering replacing elements of its existing...

76 FR 7844 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-11

.... Rassbach 509-522-6290. Amended Notices EIS No. 20100444, Final EIS, BLM, NV, Tonopah Solar Energy Crescent Dunes Solar Energy Project, a 7,680-Acre Right-of-Way (ROW) on Public Lands to Construct a Concentrated Solar Thermal Power Plant Facility, Nye County, NV, Contact: Julie Ann Smith 202-586-7668. Revision to...

76 FR 21345 - Environmental Impacts Statements;

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-15

...'Driscoll 1-866- 208-3372. EIS No. 20110112, Draft EIS, BLM, NM, HB In-Situ-Solution Mining Project.../08/2011 Pursuant to 40 CFR 1506.9. Notice In accordance with Section 309(a) of the Clean Air Act, EPA... summary of EPA's comment letters, in the Federal Register. Since February 2008, EPA has included its...

77 FR 67362 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-09

.... 20120358, Draft Supplement, BOEM, 00, Gulf of Mexico Outer Continental Shelf (OCS) Oil and Gas Lease Sales...: Aaron O. Allen 805-585-2148. EIS No. 20120360, Final EIS, BLM, 00, PROGRAMMATIC--Allocation of Oil Shale and Tar Sands Resources on Lands Administered, Propose to Amend 10 Land Use Plans in Colorado, Utah...

78 FR 54893 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-06

... make public its comments on EISs issued by other Federal agencies. EPA's comment letters on EISs are... Period Ends: 10/21/2013, Contact: Ken Corey 760-322-2070. EIS No. 20130257, Final EIS, BLM, NV... and Queens Counties, NY, Contact: Gary Kassof 212-668-7021. The U.S. Coast Guard has adopted the U.S...

77 FR 64097 - Supplemental Environmental Impact Statement to the 2011 Final EIS for the Leasing and Underground...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-18

... Final EIS for the Leasing and Underground Mining of the Greens Hollow Federal Coal Lease Tract (UTU... Mining of the Greens Hollow Federal Coal Lease Tract UTU-84102. Supplemental analyses are required to... mining methods, with foreseeable access from existing adjacent leases. The Forest Service and BLM have...

75 FR 2133 - Construction and Operation of the Quartzsite Solar Energy Project, La Paz County, AZ (DOE/EIS-0440)

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-14

... transmission system. Western is issuing this notice to inform the public and interested parties about Western's... scope, proposed action, alternatives, and issues to be addressed in the EIS. Solar Reserve has applied to the Bureau of Land Management (BLM), Yuma Field Office for a right-of-way grant for the proposed...

77 FR 4360 - Notice of Availability of the Draft Environmental Impact Statement for the Hycroft Mine Expansion...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-27

... comments related to the Hycroft Mine Expansion Draft EIS by any of the following methods: Web site: www.blm..., Nevada 89445, Attn. Kathleen Rehberg. Copies of the Hycroft Mine Expansion Draft EIS are available in the... hours. The FIRS is available 24 hours a day, 7 days a week, to leave a message or question with the...

76 FR 5201 - Notice of Realty Action: Competitive Sale of Public Lands in Monterey County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-28

... sale should be sent to the Field Manager, BLM Hollister Field Office, 20 Hamilton Court, Hollister, CA... will be provided an opportunity to submit supplemental bids. The BLM Hollister Field Office Manager... Management (BLM), Hollister Field Office, proposes to sell two separate parcels of public land totaling 80...

The adsorption of preferential binding peptides to apatite-based materials

PubMed Central

Segvich, Sharon J.; Smith, Hayes C.; Kohn, David H.

2009-01-01

The objective of this work was to identify peptide sequences with high affinity to bone-like mineral (BLM) to provide alternative design methods for functional bone regeneration peptides. Adsorption of preferential binding peptide sequences on four apatite-based substrates [BLM and three sintered apatite disks pressed from powders containing 0% CO32− (HA), 5.6% CO32− (CA5), 10.5% CO32− (CA10)] with varied compositions and morphologies was investigated. A combination of phage display, ELISA, and computational modeling was used to elucidate three 12-mer peptide sequences APWHLSSQYSRT (A), STLPI-PHEFSRE (S), and VTKHLNQISQSY (V), from 243 candidates with preferential adsorption on BLM and HA. Overall, peptides S and V have a significantly higher adsorption to the apatite-based materials in comparison to peptide A (for S vs. A, BLM p = 0.001, CA5 p < 0.001, CA10 p < 0.001, HA p = 0.038; for V vs. A, BLM p = 0.006, CA5 p = 0.033, CA10 p = 0.029). FT-IR analysis displayed carbonate levels in CA5 and CA10 dropped to approximately 1.1–2.2% after sintering, whereas SEM imaging displayed CA5 and CA10 possess distinct morphologies. Adsorption results normalized to surface area indicate that small changes in carbonate percentage at a similar morphological scale did not provide enough carbonate incorporation to show statistical differences in peptide adsorption. Because the identified peptides (S and V) have preferential binding to apatite, their use can now be investigated in bone and dentin tissue engineering, tendon and ligament repair, and enamel formation. PMID:19095299

75 FR 17951 - Notice Re-opening the Comment Period for the Draft Resource Management Plan and Draft...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-08

...The Bureau of Land Management (BLM) announces a re-opening of the comment period on the Draft Resource Management Plan (RMP) and Draft Environmental Impact Statement (EIS) for the Clear Creek Management Area (CCMA). The original notice was published in the Federal Register on December 4, 2009 [74 FR 0232] and provided for a comment period to end on March 5, 2010. The BLM is re-opening the comment period to end April 19, 2010.

78 FR 43224 - Notice of Availability of the Final Environmental Impact Statement for the Proposed Sun Valley to...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-19

...In accordance with the National Environmental Policy Act of 1969, as amended (NEPA), and the Federal Land Policy and Management Act of 1976, as amended (FLPMA), the Bureau of Land Management (BLM) has prepared a Final Environmental Impact Statement (EIS) for the proposed Sun Valley to Morgan 500/230-kilovolt (kV) Transmission Line Project (Project) and Proposed Bradshaw-Harquahala Resource Management Plan (RMP) Amendment for the BLM Hassayampa Field Office, and by this notice is announcing its availability.

Na/Ca exchange in the basolateral membrane of the A6 cell monolayer: role in Cai homeostasis.

PubMed

Brochiero, E; Raschi, C; Ehrenfeld, J

1995-05-01

The presence of a Na/Ca exchanger in A6 cells was investigated by measuring intracellular calcium (Cai) fluctuations and the 45Ca fluxes through the basolateral membranes (blm) of the cell monolayer. Removal of Na+ from the medium produced a transient increase in Cai followed by a regulatory phase returning Cai to control levels in 3-4 min, this phase being greatly accelerated (< 60 s) by NaCl addition (apparent Km of approximately 5 mM Na+). The Cai increase was only found with the Na(+)-free medium on the basolateral side of the cell monolayer. A twofold increase in the 45Ca influx was observed under these conditions. In Ca(2+)- depleted cells, the initial Cai increase after Ca2+ addition to the medium was greater when the putative Na/Ca exchanger was not functioning (i.e. in a Na(+)-free medium). 45Ca effluxes through the blm of the monolayer were greatly and transiently increased by a Na(+)-free medium on the serosal side and blocked by orthovanadate (1 mM). The Cai increased induced by a hypo-osmotic shock was greater in cells bathed in a Na(+)-medium, conditions expected to block the activity of the Na/Ca exchanger. These findings support the hypothesis that a Na/Ca exchanger is present on the blm of A6 cells and affirm its role in Cai homeostasis in steady-state conditions and following osmotic shock. In addition, a Ca2+ pump also located on the blm and Ca2+ stores sensitive to inositol 1,4,5-trisphosphate were found to be implicated in Cai homeostasis.

77 FR 61023 - Notice of Realty Action: Direct Sale of Public Land in Shasta County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-05

...] Notice of Realty Action: Direct Sale of Public Land in Shasta County, CA AGENCY: Bureau of Land...: Comments regarding the proposed sale must be received by the BLM on or before November 19, 2012. ADDRESSES: Written comments concerning the proposed sale should be sent to the Field Manager, BLM, Redding Field...

76 FR 50492 - Notice of Realty Action: Direct Sale of Public Land in San Benito County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-15

...] Notice of Realty Action: Direct Sale of Public Land in San Benito County, CA AGENCY: Bureau of Land... comments regarding the proposed sale must be received by the BLM on or before September 29, 2011. ADDRESSES: Written comments concerning the proposed sale should be sent to the Field Manager, BLM Hollister Field...

76 FR 72972 - Notice of Realty Action: Competitive Sale of Public Land in Santa Clara County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-28

...; CACA 50168] Notice of Realty Action: Competitive Sale of Public Land in Santa Clara County, CA AGENCY... the proposed sale must be received by the BLM on or before January 12, 2012. ADDRESSES: Written comments concerning the proposed sale should be sent to the Field Manager, BLM, Hollister Field Office, 20...

76 FR 68784 - Notice of Realty Action: Direct Sale of Public Land in Santa Clara County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-07

...] Notice of Realty Action: Direct Sale of Public Land in Santa Clara County, CA AGENCY: Bureau of Land... regarding the proposed sale must be received by the BLM on or before December 22, 2011. ADDRESSES: Written comments concerning the proposed sale should be sent to the Field Manager, BLM Hollister Field Office, 20...

ENVIRONMENTAL ASSESSMENT FOR THE PROPOSED WITHDRAWAL OF PUBLIC LANDS WITHIN AND SURROUNDING THE CALIENTE RAIL CORRIDOR, NEVADA

DOE Office of Scientific and Technical Information (OSTI.GOV)

DOE

2005-12-01

The purpose for agency action is to preclude surface entry and the location of new mining claims, subject to valid existing rights, within and surrounding the Caliente rail corridor as described in the Yucca Mountain FEIS (DOE 2002). This protective measure is needed to enhance the safe, efficient, and uninterrupted evaluation of land areas for potential rail alignments within the Caliente rail corridor. The evaluation will assist the DOE in determining, through the Rail Alignment environmental impact statement (EIS) process, whether to construct a branch rail line, and to provide support to the BLM in deciding whether or not tomore » reserve a ROW for the rail line under the Federal Land Policy and Management Act (FLPMA). The BLM participated as a cooperating agency in preparing this EA because it is the responsible land manager and BLM staff could contribute resource specific expertise.« less

75 FR 71454 - Meeting of the California Desert District Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-23

... lands on Friday, December 10, 2010, from 8 a.m. to 5 p.m. and will meet in formal session on Saturday, December 11, 2010, from 8 a.m. to 5 p.m. at the Riverside Marriott, 3400 Market Street, Riverside, CA 92501... BLM California state Web site at http://www.blm.gov/ca/st/en/info/rac/dac.html . SUPPLEMENTARY...

77 FR 11154 - Notice of Availability for the Eastern Interior Draft Resource Management Plan/Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-24

... BLM will announce future meetings or hearings and any other public participation activities at least..., Attention--Eastern Interior Draft RMP/EIS, Bureau of Land Management, 1150 University Avenue, Fairbanks... interior Alaska and is divided into four geographic areas: The Fortymile, Steese, Upper Black River, and...

76 FR 20715 - National Environmental Policy Act; Sounding Rockets Program; Poker Flat Research Range

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-13

...), and NASA's NEPA policy and procedures (14 CFR part 1216, subpart 1216.3), NASA intends to prepare an... to apprise interested agencies, organizations, tribal governments, and individuals of NASA's intent... significant environmental issues to be evaluated in the EIS. In cooperation with BLM, UAF, and USFWS, NASA...

78 FR 79005 - Notice of Filing of Plats of Survey; North Dakota

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-27

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLMT926000-L13100000-EI0000] Notice of Filing of Plats of Survey; North Dakota AGENCY: Bureau of Land Management, Interior. ACTION: Notice of filing of plats of survey. SUMMARY: The Bureau of Land Management (BLM) will file the plat of survey of...

77 FR 5513 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-03

... EIS, BLM, NM, HB In-Situ Solution Mining Project, Proposal to Extract the Potash Remaining in Inactive... Use Plans in Colorado, Utah, and Wyoming, Comment Period Ends: 05/02/2012, Contact: Sherri Thompson... Truck Highway 14 from Front Street in New Ulm to Nicollet County Road 6 in North Mankato, Funding, USACE...

78 FR 76176 - Notice of Filing of Plats of Survey; North Dakota

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-16

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLMT926000-L13100000-EI0000] Notice of Filing of Plats of Survey; North Dakota AGENCY: Bureau of Land Management, Interior. ACTION: Notice of filing of plats of survey. SUMMARY: The Bureau of Land Management (BLM) will file the plat of survey of...

78 FR 7808 - Notice of Intent To Prepare an Environmental Impact Statement for the Proposed Buffalo Valley...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-04

... amended, the Bureau of Land Management (BLM) Mount Lewis Field Office, Battle Mountain, Nevada, intends to... Buffalo Valley Mine Project, a proposed open pit gold mine, mill, and associated facilities, located on..._mountain_field.html . In order to be considered during the preparation of the Draft EIS, all comments must...

76 FR 65532 - Notice of Availability of the Final Environmental Impact Statement for the Proposed Sonoran Solar...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-21

... consumption. Sub-alternative Al would use PV technology instead of solar thermal technology to reduce water...; AZA34187] Notice of Availability of the Final Environmental Impact Statement for the Proposed Sonoran Solar... (BLM) has prepared a Final Environmental Impact Statement (EIS) for the Sonoran Solar Energy Project...

77 FR 13141 - Notice of Intent To Prepare a Master Leasing Plan, Amendments to the Resource Management Plans...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-05

... uses, livestock grazing, potash production, and oil and gas development. Interest in oil, gas, and... currently identified by a BLM interdisciplinary team include the following: air quality and climate change... single environmental impact statement (EIS) to consider leasing for oil and gas and potash on about 783...

77 FR 61020 - Notice of Availability of Final Environmental Impact Statement for the Sigurd to Red Butte No. 2...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-05

...; UTU-83067] Notice of Availability of Final Environmental Impact Statement for the Sigurd to Red Butte... (BLM) has prepared a Final Environmental Impact Statement (EIS) for the Sigurd to Red Butte No. 2--345...; Utah School and Institutional Trust Lands Administration; Millard, Sevier, Beaver, Iron, and Washington...

75 FR 44981 - Notice of Availability of the Final Environmental Impact Statement for Vegetation Treatments...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-30

... Treatments Using Herbicides on Bureau of Land Management Lands in Oregon AGENCY: Bureau of Land Management... Impact Statement (EIS) for Vegetation Treatments Using Herbicides on Bureau of Land Management lands in... herbicides currently in use on BLM- managed lands in Oregon and increase the number of objectives for which...

78 FR 77644 - Black Hills National Forest, South Dakota; Thunder Basin National Grassland, Wyoming; Teckla...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-24

... National Grassland, Wyoming; Teckla-Osage-Rapid City Transmission 230 kV Project AGENCY: Forest Service... (BHP) to construct and operate a 230 kilovolt (kV) transmission line between the Teckla and Osage... Management (BLM) is a cooperating agency on this EIS. The Teckla-Osage-Rapid City Transmission 230 kV Project...

75 FR 51479 - Notice of Availability of the Final Environmental Impact Statement for the Chevron Energy...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-20

... (EIS) for the Chevron Energy Solutions/Solar Millennium (CESSM), LLC's Blythe Solar Power Plant (BSPP... project and amend the CDCA Plan to prohibit solar energy projects on the project site. The BLM will take..., LVRWB09B2600] Notice of Availability of the Final Environmental Impact Statement for the Chevron Energy...

78 FR 25301 - Notice of Intent To Prepare a Draft Supplemental Environmental Impact Statement for the Ruby...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-30

...; 13-08807; MO 4500049868; TAS: 14X5017] Notice of Intent To Prepare a Draft Supplemental Environmental...: Notice of Intent. SUMMARY: As directed by the United States Court of Appeals for the Ninth Circuit, the Bureau of Land Management (BLM) is preparing a Draft Supplemental Environmental Impact Statement (EIS...

77 FR 5833 - Notice of Availability of the Draft Programmatic Environmental Impact Statement for Allocation of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-06

... of the Draft Programmatic Environmental Impact Statement for Allocation of Oil Shale and Tar Sands... of Oil Shale and Tar Sands Resources on Lands Administered by the BLM in Colorado, Utah, and Wyoming... preferred method of commenting. Mail: Addressed to: Oil Shale and Tar Sands Resources Draft Programmatic EIS...

77 FR 49825 - Notice of Availability of the Final Environmental Impact Statement for the Lost Creek Uranium In...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-17

...-166318] Notice of Availability of the Final Environmental Impact Statement for the Lost Creek Uranium In Situ Recovery Project in Sweetwater County, WY AGENCY: Bureau of Land Management, Interior. ACTION... (BLM) has prepared a Final Environmental Impact Statement (EIS) for the Lost Creek Uranium In Situ...

77 FR 68814 - Notice of Availability of the Draft Environmental Impact Statement for the Gas Hills In Situ...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-16

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLWYR05000.L51100000.GN0000.LVEMK10CW370-WYW... Recovery Uranium Project, Fremont and Natrona Counties, WY AGENCY: Bureau of Land Management, Interior... (NEPA), the Bureau of Land Management (BLM) has prepared a Draft Environmental Impact Statement (EIS...

77 FR 47839 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-10

... electronic reporting site-- https://cdx.epa.gov/epa_home.asp EIS No. 20120257, Final EIS, BR, CA, San Joaquin... No. 20120262, Draft EIS, BR, CA, San Luis Reservoir State Recreation Area Resource Management Plan...

76 FR 69279 - Notice of Intent to Prepare an Environmental Impact Statement for the Quaking Aspen Wind Energy...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-08

... to 100 1.5 megawatt (MW) to 3 MW wind turbine generators with a nameplate capacity of 250 MW of power... Intent to Prepare an Environmental Impact Statement for the Quaking Aspen Wind Energy Project, Wyoming... Statement (EIS) for the Quaking Aspen Wind Energy Project (Quaking Aspen). By this notice, BLM is: (1...

77 FR 35999 - Notice of Availability of the Proposed Resource Management Plan/Final Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-15

... addressed in the Draft EIS, including Sentinel Plain. In response, the BLM completed its inventory for... 1610.5-2. Emailed and faxed protests will not be accepted as valid protests unless the protesting party.... Authority: 40 CFR 1506.6 and 1506.10, 43 CFR 1610.2 and 1610.5. Raymond Suazo, Arizona State Director. [FR...

75 FR 57286 - Notice of Availability of the Final Environmental Impact Statement for the NextLight Renewable...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-20

... Statement for the NextLight Renewable Power, LLC, Silver State Solar Project, Clark County, NV AGENCY... Statement (EIS) for the Silver State Solar Project, Clark County, Nevada, and by this notice is announcing its availability. DATES: The BLM will not issue a final decision on the Silver State Solar Project for...

78 FR 54674 - Notice of Intent To Prepare an Environmental Impact Statement for the Proposed Gold Rock Mine...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-05

... the Proposed Gold Rock Mine Project, White Pine County, NV AGENCY: Bureau of Land Management, Interior... participation upon publication of the Draft EIS. ADDRESSES: You may submit comments related to the Gold Rock Mine Project by any of the following methods: Email: BLM_NV_EYDO_Midway_Gold_Rock[email protected] Fax: 775...

Internalisation of the bleomycin molecules responsible for bleomycin toxicity: a receptor-mediated endocytosis mechanism.

PubMed

Pron, G; Mahrour, N; Orlowski, S; Tounekti, O; Poddevin, B; Belehradek, J; Mir, L M

1999-01-01

Bleomycin (BLM) does not diffuse through the plasma membrane but nevertheless displays cytotoxic activity due to DNA break generation. The aim of the study was to describe the mechanism of BLM internalisation. We previously provided evidence for the existence of BLM-binding sites at the surface of DC-3F Chinese hamster fibroblasts, as well as of their involvement in BLM cytotoxicity on DC-3F cells and related BLM-resistant sublines. Here we report that A253 human cells and their BLM-resistant subline C-10E also possessed a membrane protein of ca. 250 kDa specifically binding BLM. Part of this C-10E cell resistance could be explained by a decrease in the number of BLM-binding sites exposed at the cell surface with respect to A253 cells. The comparison between A253 and DC-3F cells exposing a similar number of BLM-binding sites revealed that the faster the fluid phase endocytosis, the greater the cell sensitivity to BLM. Moreover, the experimental modification of endocytotic vesicle size showed that BLM cytotoxicity was directly correlated with the flux of plasma membrane area engulfed during endocytosis rather than with the fluid phase volume incorporated. Thus, BLM would be internalised by a receptor-mediated endocytosis mechanism which would first require BLM binding to its membrane receptor and then the transfer of the complex into intracellular endocytotic vesicles, followed by BLM entry into the cytosol, probably from a nonacidic compartment.

75 FR 19969 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-16

.... 20100121, Draft EIS, DOI, CA, Stanford University Habitat Conservation Plan, Authorization for Incidental... Corridor, Propose to Construct Roadway Improvements from Farm-to-Market (FM) 2920 to Interstate Highway (IH... Mott, 512- 536-5964. EIS No. 20100124, Final EIS, NPS, CA, Prisoners Harbor Coastal Wetland Restoration...

77 FR 23713 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-20

...-7104. EIS No. 20120109, Draft EIS, FHWA, OR, Salem River Crossing Project, Proposes to Build a Modified.... 20120110, Final EIS, USFWS, CA, Sears Point Wetland and Watershed Restoration Project, To Restore Tidal Wetlands and Rehabilitate Diked Wetlands, Sonoma County, CA, Review Period Ends: 05/ 21/2012, Contact: Don...

78 FR 11672 - Notice of Availability of the Final Environmental Impact Statement for the Alta East Wind Project...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-19

... Bureau of Land Management (BLM) has prepared a Proposed California Desert Conservation Area (CDCA) Plan... Ridgecrest Field Office, 300 S. Richmond Road, Ridgecrest, CA 93555, and the California Desert District... CONTACT: Jeffery Childers, telephone 951-697- 5308; address BLM California Desert District Office, 22835...

77 FR 68813 - Notice of Closure of Airport Mesa/Carizzo Creek Shooting Area in Eastern San Diego County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-16

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLCAD07000 L12200000 PA0000] Notice of... Management, Interior. ACTION: Notice. SUMMARY: The Bureau of Land Management (BLM) has closed approximately... effective as of June 19, 2012, until June 19, 2014. FOR FURTHER INFORMATION CONTACT: Daniel Steward, BLM El...

75 FR 55603 - Meeting of the California Desert District Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-13

..., October 2, 2010, from 8 a.m. to 4:30 p.m. at the BLM Needles Field Office, 1303 S Highway 95, Needles, CA 92363. Agenda topics will include updates by Council members and reports from the BLM District Manager and five field office managers. Final agenda items, including details of the field tour, will be...

78 FR 57411 - Second Call for Nominations for the Rio Grande Natural Area Commission, CO

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-18

... Nominations for the Rio Grande Natural Area Commission, CO AGENCY: Bureau of Land Management, Interior. ACTION... Specialist, BLM Front Range District Office, 3028 East Main St., Ca[ntilde]on City, CO 81212. FOR FURTHER... themselves or others. The BLM will evaluate nominees based on their education, training, experience and...

Anti-genotoxic effect of naringin against bleomycin-induced genomic damage in human lymphocytes in vitro.

PubMed

Yilmaz, Dilek; Teksoy, Ozgun; Bilaloglu, Rahmi; Çinkilic, Nilufer

2016-01-01

Naringin is a flavonoid found in grapefruit and other citrus fruits that shows antioxidant activity. The aim of the present study was to determine the anti-genotoxic and protective effects of naringin on the chemotherapeutic/radiomimetic agent bleomycin (BLM) in human blood lymphocyte cultures in vitro using micronucleus test and chromosomal aberrations (CA) assay. We tested the three doses of naringin (1, 2, 3 µg/mL) and a single dose of BLM (20 µg/mL). BLM significantly increased the total CAs and micronucleus frequency at a concentration of 20 µg/mL. Naringin did not show any toxicity in doses of 1, 2, and 3 µg/mL. Combined treatments of BLM and naringin (2 and 3 µg/mL) significantly reduced micronucleus formation. Naringin dose-dependently decreased the total chromosome aberrations frequency induced by BLM. These results indicate that naringin could prevent BLM (20 µg/mL)-induced genotoxicity.

76 FR 53453 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-26

.../2011, Contact: Sharon Seim 907-456-0501. EIS No. 20110273, Final EIS, FERC, CA, Kilarc-Cow Creek... Creek and South Cow Creek, Shasta County, CA, Wait Period Ends: 09/26/2011, Contact: Leonard Tao 1-866...

77 FR 27478 - Notice of Availability of the Draft Environmental Impact Statement and Draft Proposed California...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-10

...In accordance with the National Environmental Policy Act of 1969, as amended, and the Federal Land Policy and Management Act of 1976, as amended, the Bureau of Land Management (BLM) has prepared a Draft Proposed California Desert Conservation Area (CDCA) Plan Amendment and a Draft Environmental Impact Statement (EIS) for the Haiwee Geothermal Leasing Area (HGLA), Inyo County, California, and by this notice is announcing the opening of the comment period.

Supplement Analysis for the Transmission System Vegetation Management Program FEIS (DOE/EIS-0285/SA-40) - Allston-Keeler 500 kV Transmission Line

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hermeston, Mark W.

2002-02-19

Vegetation Management along the Allston-Keeler 500 kV Transmission Line ROW exclusive to BLM lands between 8/4 through 27/4. The proposed work will be accomplished in the indicated sections of the transmission line corridor with an average corridor width of 150 feet. BPA proposes to clear unwanted vegetation in the rights-of-ways and around tower structures that may impede the operation and maintenance of the subject transmission lines and access roads, including Reclaim and Danger Trees. The lands in this SA are administered by BLM. BPA plans to conduct vegetation control with the goal of removing tall growing vegetation that is currentlymore » or will soon be a hazard to the transmission line. BPA’s overall goal is to have low-growing plant communities along the rights-of-way to control the development of potentially threatening vegetation. All work will be executed in accordance with the National Electrical Safety Code and BPA standards. Work is to begin in March 2002. In accordance with a court injunction, no chemical treatment is planned on BLM lands.« less

Wildlife guilds in Arizona desert habitats

USGS Publications Warehouse

Short, Henry L.

1983-01-01

This report summarizes information produced from Interagency Agreement No. AA-851-IA1-27 between the Bureau of Land Management (BLM), USDI, and the Fish and Wildlife Service (FWS), USDI. The contract was instrumental in the final development of wildlife guilds for the Hualapai-Aquarius planning area of the BLM in westcentral Arizona, reported herein. The Arizona study area was selected for the application of the guilding technology because a thorough assessment of the floral and faunal resources had recently occurred in conjunction with the development of a grazing Environmental Impact Statement (EIS). Thus, the association of wildlife species with habitat type was well known, which aided in the compilation of the data base necessary for the development of guilds. Some data were also available that described the vegetative structure of habitats. This was useful in the development of a model that evaluated the quality of habitat on the basis of the diversity of cover in those habitats (Short 1982).

Challenges for the development of a biotic ligand model predicting copper toxicity in estuaries and seas.

PubMed

de Polo, Anna; Scrimshaw, Mark D

2012-02-01

An effort is ongoing to develop a biotic ligand model (BLM) that predicts copper (Cu) toxicity in estuarine and marine environments. At present, the BLM accounts for the effects of water chemistry on Cu speciation, but it does not consider the influence of water chemistry on the physiology of the organisms. We discuss how chemistry affects Cu toxicity not only by controlling its speciation, but also by affecting the osmoregulatory physiology of the organism, which varies according to salinity. In an attempt to understand the mechanisms of Cu toxicity and predict its impacts, we explore the hypothesis that the common factor linking the main toxic effects of Cu is the enzyme carbonic anhydrase (CA), because it is a Cu target with multiple functions and salinity-dependent expression and activity. According to this hypothesis, the site of action of Cu in marine fish may be not only the gill, but also the intestine, because in this tissue CA plays an important role in ion transport and water adsorption. Therefore, the BLM of Cu toxicity to marine fish should also consider the intestine as a biotic ligand. Finally, we underline the need to incorporate the osmotic gradient into the BLM calculations to account for the influence of physiology on Cu toxicity. Copyright © 2011 SETAC.

76 FR 75543 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-02

... Profile Zones (DFPZs), Lassen National Forest, Almanor Ranger District, Plumas County, CA, Comment Period... Treatment Activities, Updated and New Information, Idaho Panhandle National Forests, Priest Lake Ranger... (937) 257-5899. EIS No. 20110403, Draft EIS, USFS, CA, Creeks II Forest Restoration Project, Proposal...

75 FR 68355 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-05

... District Olympic National Forest, Olympic National Park, Jefferson County, WA, Wait Period Ends: 12/06/2010...: Lynne Urquhart 334-274-6371. EIS No. 20100431, Final EIS, USFS, WA, Dosewallips Road Washout Project, To...: 12/20/2010, Contact: Amy Henry 865-632-4045. EIS No. 20100433, Final EIS, USFS, CA, Plumas National...

76 FR 20006 - Notice of Intent To Prepare an Environmental Impact Statement for the Proposed Sun Valley to...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-11

...In compliance with the National Environmental Policy Act of 1969 (NEPA), as amended, and the Federal Land Policy and Management Act of 1976, as amended, the Bureau of Land Management (BLM) Hassayampa Field Office, Phoenix, Arizona, intends to prepare an Environmental Impact Statement (EIS) which may include discussion of an amendment to the Bradshaw-Harquahala Resource Management Plan (RMP), and by this notice is announcing the beginning of the scoping process to solicit public comments and identify issues.

77 FR 42727 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

...: https://cdx.epa.gov . EIS No. 20120230, Draft EIS, USACE, CA, Sierra Vista Specific Plan Development... No. 20120232, Final EIS, RUS, MN, Hampton--Rochester--La Crosse Transmission System Improvement... Hampton, MN and La Crosse, WI, Review Period Ends: 08/20/2012, Contact: Stephanie A. Strength 202-720-0820...

76 FR 10583 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-25

... No. 20110048, Draft EIS, DOE, 00, Disposal of Greater-Than-Class C (GTCC) Low-Level Radioactive Waste and GTCC-Like Waste, Proposed Development, Operation, and Long-Term Management of a Disposal Facility... Period Ends: 03/28/2011, Contact: Cody Wheeler 816-389-3739. EIS No. 20110051, Draft EIS, USN, CA, Marine...

76 FR 47237 - Notice of Realty Action: Direct Sale of Public Land in Monterey County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-04

...] Notice of Realty Action: Direct Sale of Public Land in Monterey County, CA AGENCY: Bureau of Land... market value of $25,000. DATES: Written comments regarding the proposed sale must be received by the BLM on or before September 19, 2011. ADDRESSES: Written comments concerning the proposed sale should be...

78 FR 58555 - Notice of Segregation of Public Lands for the Proposed Stateline Solar Farm, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-24

... Solar Energy right-of-way (ROW) application and provide for the orderly administration of public lands... solar energy project on a portion of its ROW-application area. The BLM is segregating the following...; CACA-048669] Notice of Segregation of Public Lands for the Proposed Stateline Solar Farm, CA AGENCY...

78 FR 57849 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-20

... State Solar South Project Proposed Resource Management Plan Amendment, Review Period Ends: 10/21/2013, Contact: Nancy Christ 702-515-5136. Amended Notices EIS No. 20130227, Draft EIS, NASA, CA, Proposed...

77 FR 75632 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-21

..., McCoy Solar Energy Project, Proposed Plan Amendment, Riverside County, CA, Review Period Ends: 01/ 22..., Contact: Sandra Shelin 509-527-7265. EIS No. 20120393, Final EIS, WAPA, AZ, Quartzsite Solar Energy...

75 FR 34737 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-18

... 406- 296-2536. EIS No. 20100218, Final EIS, BR, CA, North Bay Water Recycling Program (NBWRP... Green River Area Rangeland Project, Updated Information to the Analyze the Effects of Domestic Livestock...

78 FR 11899 - Notice of Realty Action: Direct Sale of Public Land in San Mateo County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-20

...] Notice of Realty Action: Direct Sale of Public Land in San Mateo County, CA AGENCY: Bureau of Land... parcels is $870,000. DATES: Comments regarding the proposed sale must be received by the BLM on or before April 8, 2013. ADDRESSES: Written comments concerning the proposed sale should be sent to the Field...

75 FR 13303 - Notice of Realty Action: Direct Sale of Public Lands in Riverside County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

...; CACA 48002] Notice of Realty Action: Direct Sale of Public Lands in Riverside County, CA AGENCY: Bureau... market value of $2,102,000. DATES: Comments regarding the proposed sale must be received by the BLM on or before May 3, 2010. ADDRESSES: Written comments concerning the proposed sale should be sent to the Field...

75 FR 65320 - Environmental Impact Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-22

... Eight Allotments, Black Hills National Forest, Pennington and Custer Counties, SD, Wait Period Ends: 11... necessary under Section 1506.3(b) of the CEQ Regulations. EIS No. 20100416, Draft EIS, WAPA, CA, Rice Solar...

77 FR 17051 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-23

..., Contact: Tina Orcutt 410-962-4290. EIS No. 20120072, Final EIS, FTA, CA, Westside Subway Extension Transit Corridor Project, Extension of the Existing Metro Purple Line and Metro Red Line Heavy Rail Subway, Los...

78 FR 42543 - Call for Nominations for the Rio Grande Natural Area Commission, Colorado

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-16

...: Send completed Council nominations to Tom Heinlein, District Manager, BLM Front Range District Office, 3028 East Main St., Ca[ntilde]on City, CO 81212. FOR FURTHER INFORMATION CONTACT: Tom Heinlein...

HINODE/EIS SPECTROSCOPIC VALIDATION OF VERY HOT PLASMA IMAGED WITH THE SOLAR DYNAMICS OBSERVATORY IN NON-FLARING ACTIVE REGION CORES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Testa, Paola; Reale, Fabio, E-mail: ptesta@cfa.harvard.edu

2012-05-01

We use coronal imaging observations with the Solar Dynamics Observatory/Atmospheric Imaging Assembly (AIA), and Hinode/Extreme-ultraviolet Imaging Spectrometer (EIS) spectral data to explore the potential of narrowband EUV imaging data for diagnosing the presence of hot (T {approx}> 5 MK) coronal plasma in active regions. We analyze observations of two active regions (AR 11281, AR 11289) with simultaneous AIA imaging and EIS spectral data, including the Ca XVII line (at 192.8 A), which is one of the few lines in the EIS spectral bands sensitive to hot coronal plasma even outside flares. After careful co-alignment of the imaging and spectral data,more » we compare the morphology in a three-color image combining the 171, 335, and 94 A AIA spectral bands, with the image obtained for Ca XVII emission from the analysis of EIS spectra. We find that in the selected active regions the Ca XVII emission is strong only in very limited areas, showing striking similarities with the features bright in the 94 A (and 335 A) AIA channels and weak in the 171 A band. We conclude that AIA imaging observations of the solar corona can be used to track hot plasma (6-8 MK), and so to study its spatial variability and temporal evolution at high spatial and temporal resolution.« less

76 FR 16812 - Notice of Realty Action: Modified Competitive Bid Sale of Public Land in Santa Clara County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-25

...: Modified Competitive Bid Sale of Public Land in Santa Clara County, CA AGENCY: Bureau of Land Management... County, California, for not less than the appraised fair market value of $41,000. The sale will be... proposed sale must be received by the BLM on or before May 9, 2011. The adjoining landowners have until 3 p...

76 FR 31627 - Notice of Realty Action: Competitive Sale of Public Lands in Lake County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-01

...] Notice of Realty Action: Competitive Sale of Public Lands in Lake County, CA AGENCY: Bureau of Land... Field Office proposes to sell an 80-acre parcel of public land in Lake County, California. The sale will... proposed sale must be received by the BLM on or before July 11, 2011. Sealed bids must be received no later...

75 FR 8935 - Environmental Impact Statements and Regulations; Availability of EPA Comments

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-26

... action. Rating LO. EIS No. 20090384, ERP No. D-NOA-K39124-CA, Fruit Growers Supply Company's Multi... action. Rating LO. Final EISs EIS No. 20090016, ERP No. F-FAA-B40165-MA, New Bedford Regional Airport...

78 FR 40497 - Notice of Availability of the Final Environmental Impact Statement/Environmental Impact Report...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-05

...In accordance with the National Environmental Policy Act of 1969, as amended (NEPA); the Federal Land Policy and Management Act of 1976, as amended; and the California Environmental Quality Act of 1970 (CEQA), the Bureau of Land Management (BLM), the United States Forest Service (USFS), and the Great Basin Unified Air Pollution Control District (GBUAPCD), a California State agency, have prepared a Final Environmental Impact Statement (EIS)/Environmental Impact Report (EIR) for the proposed Casa Diablo IV Geothermal Development Project near the town of Mammoth Lakes in Mono County, California, and by this notice are announcing its availability.

75 FR 16787 - Environmental Impact Statements and Regulations; Availability of EPA Comments

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-02

... improve environmental resources in the project area. Rating LO. EIS No. 20090451, ERP No. D-FHW-F40451-FL..., WA, OR and CA. Summary: EPA does not object to the proposed action. Rating LO. EIS No. 20100027, ERP...

76 FR 78252 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-16

..., Rubicon Trail Easement and Resource Improvement Project, Construction and Operation, Right-of-Way Grant...: Andrea Catanzaro (409) 766-6346 EIS No. 20110421, Draft EIS, USFS, CA, Greys Mountain Ecological...--La Crosse Transmission System Improvement Project, Proposed Construction and Operation of a 345...

77 FR 19281 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-30

..., FL, Central and Southern Florida Project, Broward County Water Preserve Areas, Updates Resulting from Policy Changes that occurred since 2007 Civil Works Board Approval, South Florida Water Management... for this project. EIS No. 20120089, Final EIS, USFS, CA, Greys Mountain Ecological Restoration Project...

Determining the Differential Emission Measure from EIS, XRT, and AIA

NASA Technical Reports Server (NTRS)

Winebarger, Amy R.; Warren, H.P.; Schmelz, J.

2010-01-01

This viewgraph presentation determines the Differential Emission Measure (DEM) from the EUV Imaging Spectrometer (EIS), X Ray Telescope (XRT), and Atmospheric Imaging Array (AIA). Common observations with Fe, Si, and Ca EIS lines are shown along with observations with Al-mesh, Ti-poly Al-thick and Be-thick XRT filters. Results from these observations are shown to determine what lines and filters are important to better constrain the hot component.

Validation of the Nickel Biotic Ligand Model for Locally Relevant Species in Australian Freshwaters.

PubMed

Peters, Adam; Merrington, Graham; Schlekat, Christian; De Schamphelaere, Karel; Stauber, Jennifer; Batley, Graeme; Harford, Andrew; van Dam, Rick; Pease, Ceiwen; Mooney, Tom; Warne, Michael; Hickey, Chris; Glazebrook, Peter; Chapman, John; Smith, Ross; Krassoi, Rick

2018-06-20

Australian freshwaters have relatively low water hardness and different calcium to magnesium ratios compared with those in Europe. The hardness values of a substantial proportion of Australian freshwaters fall below the application boundary of the existing European nickel Biotic Ligand Models (NiBLMs) of 2 mg Ca/L. Toxicity testing was undertaken using Hydra viridissima to assess the predictive ability of the existing NiBLM for this species in extremely soft waters. This testing revealed an increased competitive effect of calcium and magnesium with nickel for binding to the biotic ligand in soft water (<10 mg CaCO 3 /L) than at higher water hardness. Modifications were made to the NiBLM by increasing the binding constants for Ca and Mg at the biotic ligand to account for softer waters encountered in Australia and the more important competitive effect of calcium and magnesium on nickel toxicity. To validate the modified NiBLM, ecotoxicity testing was performed on five Australian test species in five different natural Australian waters. Overall, no single water chemistry parameter was able to indicate the trends in toxicity to all of the test species. The modified NiBLMs were able to predict the toxicity of nickel to the test species in the validation studies in natural waters better than the existing NiBLMs. This work suggests that the overarching mechanisms defining nickel bioavailability to freshwater species are globally similar, and that NiBLMs can be used in all freshwater systems with minor modifications. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

76 FR 34072 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-10

.... 20110178, Draft EIS, NRC, FL, Generic--License Renewal of Nuclear Plants Regarding Crystal River Unit 3.... 20110182, Final EIS, WAPA, CA, Rice Solar Energy Project, Proposed 150 megawatt Solar Energy Generating..., Access, Wildlife, Fisheries, Soil and Water, Idaho Panhandle National Forest, St. Joe Ranger District...

75 FR 24965 - Mormon Island Auxiliary Dam (MIAD) Modification Project, Sacramento and El Dorado Counties, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-06

... Folsom DS/FDR EIS/EIR considered several methods to modify MIAD to achieve Reclamation's risk standards... availability of the Final Supplemental Environmental Impact Statement/Environmental Impact Report (EIS/EIR). SUMMARY: Pursuant to the National Environmental Policy Act and the California Environmental Quality Act...

77 FR 9652 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-17

... Road-Related Impacts to Wildlife, Fish, Soil, and Water Resources and Restoration of 2010 Forest Plan..., Amendment 18A to the Fishery Management Plan for the Snapper-Grouper Fishery of the South Atlantic Region...-9235. EIS No. 20120034, Draft EIS, USFS, CA, Harris Vegetation Management Project, To Improve Forest...

78 FR 22031 - California High-Speed Rail Authority-Construction Exemption-In Merced, Madera and Fresno Counties...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-12

... High-Speed Rail Authority--Construction Exemption--In Merced, Madera and Fresno Counties, CA AGENCY... High-Speed Rail Authority (Authority). This Final EIS is titled ``California High-Speed Train: Merced... Final EIS assesses the potential environmental impacts of constructing and operating a high-speed...

75 FR 80807 - Environmental Impact Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-23

..., CA, East County Substation/Tule Wind/ Energia Sierra Juarez Gen-Tie Projects, Construction and.... 20100462, Draft EIS, FWS, CA, Santa Clara Valley Habitat Plan, To Protect and Enhance Ecological Diversity...

Emotional intelligence and academic performance in first and final year medical students: a cross-sectional study.

PubMed

Chew, Boon How; Zain, Azhar Md; Hassan, Faezah

2013-03-27

Research on emotional intelligence (EI) suggests that it is associated with more pro-social behavior, better academic performance and improved empathy towards patients. In medical education and clinical practice, EI has been related to higher academic achievement and improved doctor-patient relationships. This study examined the effect of EI on academic performance in first- and final-year medical students in Malaysia. This was a cross-sectional study using an objectively-scored measure of EI, the Mayer-Salovey-Caruso Emotional Intelligence Test (MSCEIT). Academic performance of medical school students was measured using continuous assessment (CA) and final examination (FE) results. The first- and final-year students were invited to participate during their second semester. Students answered a paper-based demographic questionnaire and completed the online MSCEIT on their own. Relationships between the total MSCEIT score to academic performance were examined using multivariate analyses. A total of 163 (84 year one and 79 year five) medical students participated (response rate of 66.0%). The gender and ethnic distribution were representative of the student population. The total EI score was a predictor of good overall CA (OR 1.01), a negative predictor of poor result in overall CA (OR 0.97), a predictor of the good overall FE result (OR 1.07) and was significantly related to the final-year FE marks (adjusted R(2) = 0.43). Medical students who were more emotionally intelligent performed better in both the continuous assessments and the final professional examination. Therefore, it is possible that emotional skill development may enhance medical students' academic performance.

75 FR 19992 - Notice of Availability of the Supplemental Draft Environmental Impact Statement for the Proposed...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-16

... Statement for the Proposed Ivanpah Solar Electric Generation System Project, San Bernardino County, CA... Notice of Availability of the Draft Ivanpah Solar Electric Generation System EIS and the Draft California... prepared a Supplemental Draft Environmental Impact Statement (EIS) for the Proposed Ivanpah Solar Electric...

75 FR 6218 - New Melones Lake Area Resource Management Plan, Tuolumne and Calaveras Counties, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-08

... DEPARTMENT OF THE INTERIOR Bureau of Reclamation New Melones Lake Area Resource Management Plan...) has made available for public review a Final RMP/EIS for the New Melones Lake Area. The Final RMP/EIS... Dan Holsapple, Acting New Melones Resource Manager, Bureau of Reclamation, at 209-536- 9094...

75 FR 14594 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-26

... Forest, Mono County, CA and Lyon, Douglas, and Mineral Counties, NV, Wait Period Ends: 04/26/2010.../ 2010, Contact: Douglas Gober, 208-476-4541. EIS No. 20100089, Draft EIS, STB, AK, Port MacKenzie Rail..., Richland, Benton County, WA, Comment Period Ends: 05/03/2010, Contact: Mary Beth Burandi, 888-829- 6347...

Emotional intelligence and academic performance in first and final year medical students: a cross-sectional study

PubMed Central

2013-01-01

Background Research on emotional intelligence (EI) suggests that it is associated with more pro-social behavior, better academic performance and improved empathy towards patients. In medical education and clinical practice, EI has been related to higher academic achievement and improved doctor-patient relationships. This study examined the effect of EI on academic performance in first- and final-year medical students in Malaysia. Methods This was a cross-sectional study using an objectively-scored measure of EI, the Mayer-Salovey-Caruso Emotional Intelligence Test (MSCEIT). Academic performance of medical school students was measured using continuous assessment (CA) and final examination (FE) results. The first- and final-year students were invited to participate during their second semester. Students answered a paper-based demographic questionnaire and completed the online MSCEIT on their own. Relationships between the total MSCEIT score to academic performance were examined using multivariate analyses. Results A total of 163 (84 year one and 79 year five) medical students participated (response rate of 66.0%). The gender and ethnic distribution were representative of the student population. The total EI score was a predictor of good overall CA (OR 1.01), a negative predictor of poor result in overall CA (OR 0.97), a predictor of the good overall FE result (OR 1.07) and was significantly related to the final-year FE marks (adjusted R2 = 0.43). Conclusions Medical students who were more emotionally intelligent performed better in both the continuous assessments and the final professional examination. Therefore, it is possible that emotional skill development may enhance medical students’ academic performance. PMID:23537129

Penicillin biosensor based on a capacitive field-effect structure functionalized with a dendrimer/carbon nanotube multilayer.

PubMed

Siqueira, José R; Abouzar, Maryam H; Poghossian, Arshak; Zucolotto, Valtencir; Oliveira, Osvaldo N; Schöning, Michael J

2009-10-15

Silicon-based sensors incorporating biomolecules are advantageous for processing and possible biological recognition in a small, reliable and rugged manufactured device. In this study, we report on the functionalization of field-effect (bio-)chemical sensors with layer-by-layer (LbL) films containing single-walled carbon nanotubes (SWNTs) and polyamidoamine (PAMAM) dendrimers. A capacitive electrolyte-insulator-semiconductor (EIS) structure modified with carbon nanotubes (EIS-NT) was built, which could be used as a penicillin biosensor. From atomic force microscopy (AFM) and field-emission scanning electron microscopy (FESEM) images, the LbL films were shown to be highly porous due to interpenetration of SWNTs into the dendrimer layers. Capacitance-voltage (C/V) measurements pointed to a high pH sensitivity of ca. 55 mV/pH for the EIS-NT structures. The biosensing ability towards penicillin of an EIS-NT-penicillinase biosensor was also observed as the flat-band voltage shifted to lower potentials at different penicillin concentrations. A dynamic response of penicillin concentrations, ranging from 5.0 microM to 25 mM, was evaluated for an EIS-NT with the penicillinase enzyme immobilized onto the surfaces, via constant-capacitance (ConCap) measurements, achieving a sensitivity of ca. 116 mV/decade. The presence of the nanostructured PAMAM/SWNT LbL film led to sensors with higher sensitivity and better performance.

75 FR 7570 - Notice of Public Hearings for the Draft Environmental Impact Statement for the Silver Strand...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-22

... Impact Statement for the Silver Strand Training Complex, San Diego, CA; Correction AGENCY: Department of... Impact Statement for the Silver Strand Training Complex, San Diego, CA. The document contained incorrect.... Kent Randall, SSTC EIS Project Manager, 1220 Pacific Highway, Building 1, 5th Floor, San Diego, CA...

Nitroprusside inhibits calcium-induced impairment of red blood cell deformability.

PubMed

Barodka, Viachaslau; Mohanty, Joy G; Mustafa, Asif K; Santhanam, Lakshmi; Nyhan, Aoibhinn; Bhunia, Anil K; Sikka, Gautam; Nyhan, Daniel; Berkowitz, Dan E; Rifkind, Joseph M

2014-02-01

Red blood cell (RBC) deformation is critical for microvascular perfusion and oxygen delivery to tissues. Abnormalities in RBC deformability have been observed in aging, sickle cell disease, diabetes, and preeclampsia. Although nitric oxide (NO) prevents decreases in RBC deformability, the underlying mechanism is unknown. As an experimental model, we used ionophore A23187-mediated calcium influx in RBCs to reduce their deformability and investigated the role of NO donor sodium nitroprusside (SNP) and KCa3.1 (Gardos) channel blockers on RBC deformability (measured as elongation index [EI] by microfluidic ektacytometry). RBC intracellular Ca(2+) and extracellular K(+) were measured by inductively coupled plasma mass spectrometry and potassium ion selective electrode, respectively. SNP treatment of RBCs blocked the Ca(2+) (approx. 10 μmol/L)-induced decrease in RBC deformability (EI 0.34 ± 0.02 vs. 0.09 ± 0.01, control vs. Ca(2+) loaded, p < 0.001; and EI 0.37 ± 0.02 vs. 0.30 ± 0.01, SNP vs. SNP plus Ca(2+) loaded) as well as Ca(2+) influx and K(+) efflux. The SNP effect was similar to that observed after pharmacologic blockade of the KCa3.1 channel (with charybdotoxin or extracellular medium containing isotonic K(+) concentration). In RBCs from KCa3.1(-/-) mice, 10 μmol/L Ca(2+) loading did not decrease cellular deformability. A preliminary attempt to address the molecular mechanism of SNP protection suggests the involvement of cell surface thiols. Our results suggest that nitroprusside treatment of RBCs may protect them from intracellular calcium increase-mediated stiffness, which may occur during microvascular perfusion in diseased states, as well as during RBC storage. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.

75 FR 18235 - Paiute Cutthroat Trout Restoration Project, Alpine County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-09

...] Paiute Cutthroat Trout Restoration Project, Alpine County, CA AGENCY: Fish and Wildlife Service, Interior... availability of the Paiute Cutthroat Trout Restoration Project Final Environmental Impact Statement (EIS... cutthroat trout to the species historical range within the Silver King Creek watershed, Alpine County...

Alleviation of Cu and Pb rhizotoxicities in cowpea (Vigna unguiculata) as related to ion activities at root-cell plasma membrane surface

USDA-ARS?s Scientific Manuscript database

Cations, such as Ca and Mg, are generally thought to alleviate toxicities of trace metals through site-specific competition (as incorporated in the biotic ligand model, BLM). Short term (48 h) experiments were conducted using cowpea (Vigna unguiculata L. Walp.) seedlings in simple nutrient solution...

75 FR 62136 - Notice Extending Scoping Period for the Notice of Intent To Prepare an Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-07

... the Walker Ridge Wind Project, Lake and Colusa Counties, CA AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: The Bureau of Land Management (BLM) announces the extension of the... DEPARTMENT OF THE INTERIOR Bureau of Land Management [L51010000.FX0000 LVRWB10B4040 LLCAC05000...

78 FR 32688 - Notice of Realty Action, Segregation Terminated, Direct Sale of Public Land in San Bernardino...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-31

...] Notice of Realty Action, Segregation Terminated, Direct Sale of Public Land in San Bernardino County, CA... for the appraised fair market value of $55,000. DATES: Comments regarding the proposed sale must be received by the BLM on or before July 15, 2013. ADDRESSES: Written comments concerning the proposed sale...

77 FR 25734 - 30-Day Extension of Call for Nominations for the Bureau of Land Management's California Desert...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-01

... Extension of Call for Nominations for the Bureau of Land Management's California Desert District Advisory... Management's (BLM) California Desert District is extending the call for nominations from the public for six..., California Desert District Office, 22835 Calle San Juan De Los Lagos, Moreno Valley, CA 92553. FOR FURTHER...

75 FR 28650 - Notice of Realty Action: Proposed Direct Sale of Public Lands in Riverside County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-21

... in Riverside County, California to Cocopah Nurseries, Inc. for the appraised fair market value of $77... INFORMATION: The following described public land is being proposed for direct sale to Cocopah Nurseries, Inc... isolated parcel of land which lacks legal access. The BLM is proposing a direct sale to Cocopah Nurseries...

75 FR 32812 - Notice of Proposed Reinstatement of Terminated Oil and Gas Lease CACA 44900, California

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-09

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [CA-920-1310-FI; CACA 44900] Notice of Proposed Reinstatement of Terminated Oil and Gas Lease CACA 44900, California AGENCY: Bureau of Land... Management (BLM) received a petition for reinstatement of oil and gas lease CACA 44900 from NW. Lost Hills...

75 FR 2541 - Environmental Impact Statements and Regulations; Availability of EPA Comments

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-15

..., 158th Fighter Wing Vermont Air National Guard Project, Proposed Realignment of National Guard Avenue and... the preparing agency. EIS No. 20090401, ERP No. F-IBR-K39118-CA, Delta-Mendota Canal/ California... Delta-Mendota Water Authority Project, Central Valley Project, Alameda and San Joaquin Counties, CA...

76 FR 6154 - Notice of Realty Action: Modified Competitive Bid Sale of Public Lands in Santa Cruz County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-03

...; CACA 50168 06] Notice of Realty Action: Modified Competitive Bid Sale of Public Lands in Santa Cruz... fair market value of $53,000. The sale will be conducted as a modified competitive bid auction, whereby... public land. DATES: Written comments regarding this proposed sale must be received by the BLM on or...

An electrocatalytic oxidation and voltammetric method using a chemically reduced graphene oxide film for the determination of caffeic acid.

PubMed

Vilian, A T Ezhil; Chen, Shen-Ming; Chen, Ying-Hui; Ali, M Ajmal; Al-Hemaid, Fahad M A

2014-06-01

The present work describes the characterization of a chemically reduced graphene oxide (CRGO) modified glassy carbon electrode (GCE) for electrochemical investigation of caffeic acid (CA). Cyclic voltammetry (CV), differential pulse voltammetry (DPV), amperometry, and electrochemical impedance spectroscopy (EIS) techniques were used to characterize the properties of the electrode. There was an obvious enhancement of the current response and a decreased over potential for the oxidation of CA. The interfacial electron transfer rate of CA was studied by EIS. Under optimal conditions, the CRGO displayed a linear response range of 1×10(-8) to 8×10(-4) M and the detection limit was 2×10(-9) M (S/N=3), with a sensitivity of 192.21 μA mM(-1) cm(-2) at an applied potential of +0.2V (vs. Ag/AgCl reference), which suggests that the CRGO is a promising sensing materials for the electrochemical investigation of CA. The results showed the good sensitivity, selectivity and high reproducibility of the CRGO modified electrode. Moreover, this modified electrode was further applied to investigate the CA in real samples of wine with satisfactory results. Copyright © 2014 Elsevier Inc. All rights reserved.

Geothermal NEPA Database on OpenEI (Poster)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Young, K. R.; Levine, A.

2014-09-01

The National Renewable Energy Laboratory (NREL) developed the Geothermal National Environmental Policy Act (NEPA) Database as a platform for government agencies and industry to access and maintain information related to geothermal NEPA documents. The data were collected to inform analyses of NEPA timelines, and the collected data were made publically available via this tool in case others might find the data useful. NREL staff and contractors collected documents from agency websites, during visits to the two busiest Bureau of Land Management (BLM) field offices for geothermal development, and through email and phone call requests from other BLM field offices. Theymore » then entered the information into the database, hosted by Open Energy Information (http://en.openei.org/wiki/RAPID/NEPA). The long-term success of the project will depend on the willingness of federal agencies, industry, and others to populate the database with NEPA and related documents, and to use the data for their own analyses. As the information and capabilities of the database expand, developers and agencies can save time on new NEPA reports by accessing a single location to research related activities, their potential impacts, and previously proposed and imposed mitigation measures. NREL used a wiki platform to allow industry and agencies to maintain the content in the future so that it continues to provide relevant and accurate information to users.« less

77 FR 70182 - Impact Report for the Proposed Stateline Solar Farm, San Bernardino County, CA and Draft...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-23

... application for a ROW grant to construct, operate, maintain, and decommission a photovoltaic solar energy... Proposed Stateline Solar Farm, San Bernardino County, CA and Draft California Desert Conservation Area Land... Statement/Environmental Impact Report (EIS/EIR) for the Stateline Solar Farm Project (Stateline) and by this...

75 FR 21341 - Millerton Lake Resource Management Plan/General Plan (RMP/GP), Madera and Fresno Counties, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-23

... DEPARTMENT OF THE INTERIOR Bureau of Reclamation Millerton Lake Resource Management Plan/General Plan (RMP/GP), Madera and Fresno Counties, CA AGENCY: Bureau of Reclamation, Interior. ACTION: Notice... adoption of the RMP/GP. The Final EIS/EIR outlines the formulation and evaluation of alternatives designed...

In Situ Neutron Diffraction of Rare-Earth Phosphate Proton Conductors Sr/Ca-doped LaPO4 at Elevated Temperatures

NASA Astrophysics Data System (ADS)

Al-Wahish, Amal; Al-Binni, Usama; Bridges, C. A.; Huq, A.; Bi, Z.; Paranthaman, M. P.; Tang, S.; Kaiser, H.; Mandrus, D.

Acceptor-doped lanthanum orthophosphates are potential candidate electrolytes for proton ceramic fuel cells. We combined neutron powder diffraction (NPD) at elevated temperatures up to 800° C , X-ray powder diffraction (XRD) and scanning electron microscopy (SEM) to investigate the crystal structure, defect structure, thermal stability and surface topography. NPD shows an average bond length distortion in the hydrated samples. We employed Quasi-Elastic Neutron Scattering (QENS) and electrochemical impedance spectroscopy (EIS) to study the proton dynamics of the rare-earth phosphate proton conductors 4.2% Sr/Ca-doped LaPO4. We determined the bulk diffusion and the self-diffusion coefficients. Our results show that QENS and EIS are probing fundamentally different proton diffusion processes. Supported by the U.S. Department of Energy.

Chk1 protects against chromatin bridges by constitutively phosphorylating BLM serine 502 to inhibit BLM degradation.

PubMed

Petsalaki, Eleni; Dandoulaki, Maria; Morrice, Nick; Zachos, George

2014-09-15

Chromatin bridges represent incompletely segregated chromosomal DNA connecting the anaphase poles and can result in chromosome breakage. The Bloom's syndrome protein helicase (BLM, also known as BLMH) suppresses formation of chromatin bridges. Here, we show that cells deficient in checkpoint kinase 1 (Chk1, also known as CHEK1) exhibit higher frequency of chromatin bridges and reduced BLM protein levels compared to controls. Chk1 inhibition leads to BLM ubiquitylation and proteasomal degradation during interphase. Furthermore, Chk1 constitutively phosphorylates human BLM at serine 502 (S502) and phosphorylated BLM localises to chromatin bridges. Mutation of S502 to a non-phosphorylatable alanine residue (BLM-S502A) reduces the stability of BLM, whereas expression of a phospho-mimicking BLM-S502D, in which S502 is mutated to aspartic acid, stabilises BLM and prevents chromatin bridges in Chk1-deficient cells. In addition, wild-type but not BLM-S502D associates with cullin 3, and cullin 3 depletion rescues BLM accumulation and localisation to chromatin bridges after Chk1 inhibition. We propose that Chk1 phosphorylates BLM-S502 to inhibit cullin-3-mediated BLM degradation during interphase. These results suggest that Chk1 prevents deleterious anaphase bridges by stabilising BLM. © 2014. Published by The Company of Biologists Ltd.

Development of a Multi-Species Biotic Ligand Model Predicting the Toxicity of Trivalent Chromium to Barley Root Elongation in Solution Culture

PubMed Central

Song, Ningning; Zhong, Xu; Li, Bo; Li, Jumei; Wei, Dongpu; Ma, Yibing

2014-01-01

Little knowledge is available about the influence of cation competition and metal speciation on trivalent chromium (Cr(III)) toxicity. In the present study, the effects of pH and selected cations on the toxicity of trivalent chromium (Cr(III)) to barley (Hordeum vulgare) root elongation were investigated to develop an appropriate biotic ligand model (BLM). Results showed that the toxicity of Cr(III) decreased with increasing activity of Ca2+ and Mg2+ but not with K+ and Na+. The effect of pH on Cr(III) toxicity to barley root elongation could be explained by H+ competition with Cr3+ bound to a biotic ligand (BL) as well as by the concomitant toxicity of CrOH2+ in solution culture. Stability constants were obtained for the binding of Cr3+, CrOH2+, Ca2+, Mg2+ and H+ with binding ligand: log KCrBL 7.34, log KCrOHBL 5.35, log KCaBL 2.64, log KMgBL 2.98, and log KHBL 4.74. On the basis of those estimated parameters, a BLM was successfully developed to predict Cr(III) toxicity to barley root elongation as a function of solution characteristics. PMID:25119269

Development of a multi-species biotic ligand model predicting the toxicity of trivalent chromium to barley root elongation in solution culture.

PubMed

Song, Ningning; Zhong, Xu; Li, Bo; Li, Jumei; Wei, Dongpu; Ma, Yibing

2014-01-01

Little knowledge is available about the influence of cation competition and metal speciation on trivalent chromium (Cr(III)) toxicity. In the present study, the effects of pH and selected cations on the toxicity of trivalent chromium (Cr(III)) to barley (Hordeum vulgare) root elongation were investigated to develop an appropriate biotic ligand model (BLM). Results showed that the toxicity of Cr(III) decreased with increasing activity of Ca(2+) and Mg(2+) but not with K(+) and Na(+). The effect of pH on Cr(III) toxicity to barley root elongation could be explained by H(+) competition with Cr(3+) bound to a biotic ligand (BL) as well as by the concomitant toxicity of CrOH(2+) in solution culture. Stability constants were obtained for the binding of Cr(3+), CrOH(2+), Ca(2+), Mg(2+) and H(+) with binding ligand: log KCrBL 7.34, log KCrOHBL 5.35, log KCaBL 2.64, log KMgBL 2.98, and log KHBL 4.74. On the basis of those estimated parameters, a BLM was successfully developed to predict Cr(III) toxicity to barley root elongation as a function of solution characteristics.

The 15th Internatonal Conference Quality in Resarch (Qir) 2017 Preparation and Ionic Conductivity of Li3.9Ca0.1Ti5O12 Using Waste Chicken Eggshells as ca Source for Anode Material of Lithium-Ion Batteries

NASA Astrophysics Data System (ADS)

Subhan, Achmad; Setiawan, Dedy; Ahmiatri Saptari, Sitti

2018-03-01

Li3.9Ca0.1Ti5O12 has been synthesized as anode material for lithium-ion batteries parallel with Li4Ti5O12 anode material using solid state reaction method in an air atmosphere. LiOH.H2O, TiO2, and waste chicken eggshells in the form of CaCO3 were chosen as sources of Li, Ti, and Ca respectively and prepared using stoichiometric. The phase structure, morphology, and electrochemical impedance of as-prepared samples were characterized using XRD, SEM, and EIS. The XRD characterization revealed that in Li3.9Ca0.1Ti5O12 sample, all amount of dopant had entered the lattice structure of Li4Ti5O12. The EDX image also detect the existence of Ca in the structure of Li3.9Ca0.1Ti5O12. The EIS characterization revealed that the Li3.9Ca0.1Ti5O12 sample had lower electrochemical impedance compared to the Li4Ti5O12 sample. The diffusion coefficient were obtained by Faraday’s method, and exhibited that the Li3.9Ca0.1Ti5O12 sample (1.46986 × 10-12 cm2/s) had higher ionic conductivity than the Li4Ti5O12 sample (4.40995 × 10-16 cm2/s). According to the cycle performance test, the Li3.9Ca0.1Ti5O12 sample also had higher charge-discharge capacity and stability compared to the Li4Ti5O12 sample.

78 FR 68466 - BLM Director's Response to the Idaho Governor's Appeal of the BLM Idaho State Director's Governor...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-14

... Bureau of Land Management (BLM) is publishing this notice to explain why the BLM Director is denying the...] BLM Director's Response to the Idaho Governor's Appeal of the BLM Idaho State Director's Governor's... (Finding) to the BLM Idaho State Director (State Director). The State Director determined the Governor's...

76 FR 3157 - Joint Operations Center Relocation Project, Sacramento County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-19

... Water Resources (DWR), the lead State agency, will prepare a joint EIS/EIR for the proposed Joint... following departments: DWR's Division of Operations and Maintenance, Operations Control Office, State Water...

76 FR 18217 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-01

.... 20110089, Draft EIS, HUD, CA, West Coast Recycling Group Metal Recycling Facility Project, Proposal to Develop and Operate a Scrap Metal Shredding and Recycling Facility at the Port of West Sacramento, Yolo...

Army Force Planning Cost Handbook (AFPCH)

DTIC Science & Technology

1982-11-01

FY82 procurement costs extracted form the POM Procurement Data Base. The weapon system unit cost definition most nearly describing these prices is...Is’ 11 il 1 ~~~~l OIL ~ JII~II It I IM u 14 1W x A I- 44 I It N IIt a-lIt IIr- a00 ~ a a I . 1 I1,35O @ I1 810R II u ILI i41 Iis LL, It-I - C I Ca...NW 4 m I IV4 AI 3 0 MI ha111 IA177 00 -. 1- 1 -. l 6 A s𔃺 ca $soon~. I _ 44 gKK4 oil ’r Ei I~~ EI I ~ a BH* .0 0 ’ 4 cp. oM I’- F 111 jIr a;I HI amm

Selective tumor cell targeting by the disaccharide moiety of bleomycin.

PubMed

Yu, Zhiqiang; Schmaltz, Ryan M; Bozeman, Trevor C; Paul, Rakesh; Rishel, Michael J; Tsosie, Krystal S; Hecht, Sidney M

2013-02-27

In a recent study, the well-documented tumor targeting properties of the antitumor agent bleomycin (BLM) were studied in cell culture using microbubbles that had been derivatized with multiple copies of BLM. It was shown that BLM selectively targeted MCF-7 human breast carcinoma cells but not the "normal" breast cell line MCF-10A. Furthermore, it was found that the BLM analogue deglycobleomycin, which lacks the disaccharide moiety of BLM, did not target either cell line, indicating that the BLM disaccharide moiety is necessary for tumor selectivity. Not resolved in the earlier study were the issues of whether the BLM disaccharide moiety alone is sufficient for tumor cell targeting and the possible cellular uptake of the disaccharide. In the present study, we conjugated BLM, deglycoBLM, and BLM disaccharide to the cyanine dye Cy5**. It was found that the BLM and BLM disaccharide conjugates, but not the deglycoBLM conjugate, bound selectively to MCF-7 cells and were internalized. The same was also true for the prostate cancer cell line DU-145 (but not for normal PZ-HPV-7 prostate cells) and for the pancreatic cancer cell line BxPC-3 (but not for normal SVR A221a pancreas cells). The targeting efficiency of the disaccharide was only slightly less than that of BLM in MCF-7 and DU-145 cells and comparable to that of BLM in BxPC-3 cells. These results establish that the BLM disaccharide is both necessary and sufficient for tumor cell targeting, a finding with obvious implications for the design of novel tumor imaging and therapeutic agents.

78 FR 4435 - BLM Director's Response to the Alaska Governor's Appeal of the BLM Alaska State Director's...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-22

... Bureau of Land Management (BLM) is publishing this notice to explain why the BLM Director is rejecting... Director's Response to the Alaska Governor's Appeal of the BLM Alaska State Director's Governor's... the BLM Alaska State Director. The State Director determined the Governor's Finding was outside the...

The effects of voluntary running exercise coincidence with social isolation after early weaning on monoaminergic axonal development.

PubMed

Ishikawa, J; Ishikawa, A

2013-01-29

The axonal development of serotonin (5-HT)-, noradrenaline (NA)-, or tyrosine hydroxylase (TH)-containing monoaminergic neurons is affected by rearing conditions during the juvenile period. Impaired monoaminergic axonal development is implicated in the pathophysiology of emotional and cognitive dysfunction. On the other hand, exercise may have beneficial effects on emotional and learning performance in adults. We have examined whether voluntary running exercise during social isolation after early weaning (early weaning/social isolation; EI) from postnatal day (PD) 14-28 could prevent the impaired monoaminergic axonal development associated with EI. Compared with control animals reared with their dam and siblings until PD28, the EI animals showed lower density of 5-HT and NA axons in the dorsal-medial prefrontal cortex (mPFC) and basolateral nucleus of the amygdala and of NA- and TH-containing axons in the ventral-mPFC. These adverse effects of EI were not observed in rats taking part in voluntary running (EI+R) when these animals were compared to controls. The 5-HT axon density in the ventral-mPFC was significantly higher in the EI+R rats than that in the EI rats, although both these values were significantly lower than those in the control rats. The density of monoaminergic axons in the dentate gyrus and CA3 of the hippocampus was not affected by either EI or EI+R. These results suggest that the beneficial effects of voluntary running may be because of the modulation of monoaminergic axonal morphology. Our findings will hopefully provide the basis for future research into the beneficial effects of voluntary exercise during the juvenile period on brain development and emotional and cognitive performance. Copyright © 2012 IBRO. Published by Elsevier Ltd. All rights reserved.

Beyond excitation/inhibition imbalance in multidimensional models of neural circuit changes in brain disorders.

PubMed

O'Donnell, Cian; Gonçalves, J Tiago; Portera-Cailliau, Carlos; Sejnowski, Terrence J

2017-10-11

A leading theory holds that neurodevelopmental brain disorders arise from imbalances in excitatory and inhibitory (E/I) brain circuitry. However, it is unclear whether this one-dimensional model is rich enough to capture the multiple neural circuit alterations underlying brain disorders. Here, we combined computational simulations with analysis of in vivo two-photon Ca 2+ imaging data from somatosensory cortex of Fmr1 knock-out (KO) mice, a model of Fragile-X Syndrome, to test the E/I imbalance theory. We found that: (1) The E/I imbalance model cannot account for joint alterations in the observed neural firing rates and correlations; (2) Neural circuit function is vastly more sensitive to changes in some cellular components over others; (3) The direction of circuit alterations in Fmr1 KO mice changes across development. These findings suggest that the basic E/I imbalance model should be updated to higher dimensional models that can better capture the multidimensional computational functions of neural circuits.

Beyond excitation/inhibition imbalance in multidimensional models of neural circuit changes in brain disorders

PubMed Central

Gonçalves, J Tiago; Portera-Cailliau, Carlos

2017-01-01

A leading theory holds that neurodevelopmental brain disorders arise from imbalances in excitatory and inhibitory (E/I) brain circuitry. However, it is unclear whether this one-dimensional model is rich enough to capture the multiple neural circuit alterations underlying brain disorders. Here, we combined computational simulations with analysis of in vivo two-photon Ca2+ imaging data from somatosensory cortex of Fmr1 knock-out (KO) mice, a model of Fragile-X Syndrome, to test the E/I imbalance theory. We found that: (1) The E/I imbalance model cannot account for joint alterations in the observed neural firing rates and correlations; (2) Neural circuit function is vastly more sensitive to changes in some cellular components over others; (3) The direction of circuit alterations in Fmr1 KO mice changes across development. These findings suggest that the basic E/I imbalance model should be updated to higher dimensional models that can better capture the multidimensional computational functions of neural circuits. PMID:29019321

Ca Addition Effects on the Microstructure, Tensile and Corrosion Properties of Mg Matrix Alloy Containing 8 wt.% Mg2Si

NASA Astrophysics Data System (ADS)

Lotfpour, M.; Emamy, M.; Dehghanian, C.; Pourbahari, B.

2018-02-01

The microstructure, tensile properties and corrosion behavior of the Mg-8 wt.% Mg2Si-x%Ca alloy have been studied by the use of optical microscopy, scanning electron microscopy equipped with energy-dispersive spectroscopy, x-ray diffraction, standard tensile testing, polarization test and electrochemical impedance spectroscopy (EIS) measurements. Microstructural studies indicated that Ca modifies both primary and eutectic Mg2Si phase. It was found that the average size of primary Mg2Si particles is about 60 μm, which is dropped by about 82% in the alloy containing 0.05 wt.% Ca. By the addition of different Ca contents, Ca-rich intermetallics (i.e., CaSi2 and CaMgSi) were formed. The modification mechanism of adding Ca during solidification was found to be due to the strong effect of CaMgSi phase as a heterogonous nucleation site, apart from CaSi2 which was reported before, for Mg2Si intermetallics. Tensile testing results ascertained that Ca addition enhances both ultimate tensile strength (UTS) and elongation values. The optimum amount of Ca was found to be 0.1 wt.%, which improved UTS and elongation values from about 130 MPa and 2% to 165 MPa and 5.5%, whereas more Ca addition (i.e., 3 wt.%) reduced the tensile properties of the alloy to about 105 MPa and 1.8%, which can be due to the formation of CaMgSi intermetallics with deteriorating needle-like morphology. Polarization and EIS tests also showed that the Mg-3%Si-0.5%Ca alloy pronounces as the best anti-corrosion alloy. Nevertheless, further added Ca (up to 3 wt.%) deteriorated the corrosion resistance due to predominance of worse galvanic coupling effect stemmed from the presence of stronger CaMgSi cathode in comparison with Mg2Si. With higher Ca additions, an adverse effect was seen on corrosion resistance of the Mg-3%Si alloy, as a result of forming a weak film on the alloy specimen surface.

43 CFR 3277.12 - What will BLM do if I do not comply with all BLM requirements pertaining to utilization operations?

Code of Federal Regulations, 2011 CFR

2011-10-01

... all BLM requirements pertaining to utilization operations? 3277.12 Section 3277.12 Public Lands... § 3277.12 What will BLM do if I do not comply with all BLM requirements pertaining to utilization... corrective action within a specific time period. If the noncompliance continues or is serious in nature, BLM...

The function of Xenopus Bloom's syndrome protein homolog (xBLM) in DNA replication

PubMed Central

Liao, Shuren; Graham, Jeanine; Yan, Hong

2000-01-01

The Bloom's syndrome gene (BLM) plays a pivotal role in the maintenance of genomic stability in somatic cells. It encodes a DNA helicase (BLM) of the RecQ family, but the exact function of BLM remains elusive. To study this question, we have cloned the BLM homolog of the frog Xenopus laevis (xBLM) and have raised antibodies to it. Immunodepletion of xBLM from a Xenopus egg extract severely inhibits the replication of DNA in reconstituted nuclei. Moreover, the inhibition can be rescued by the addition of the recombinant xBLM protein. These results provide the first direct evidence that BLM plays an important role in DNA replication, suggesting that Bloom's syndrome may be the consequence of defective DNA replication. PMID:11040210

78 FR 13873 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-01

..., CA, Middle Fork American River Project, Review Period Ends: 04/01/2013, Contact: Matt Buhyoff 202-502.... 20130048, Draft EIS, BOEM, 00, Gulf of Mexico OCS Oil and Gas Lease Sales: 2014 and 2016 Eastern Planning...

THE MYSTERIOUS CASE OF THE SOLAR ARGON ABUNDANCE NEAR SUNSPOTS IN FLARES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Doschek, G. A.; Warren, H. P.

Recently we discussed an enhancement of the abundance of Ar xiv relative to Ca xiv near a sunspot during a flare, observed in spectra recorded by the Extreme-ultraviolet Imaging Spectrometer (EIS) on the Hinode spacecraft. The observed Ar xiv/Ca xiv ratio yields an argon/calcium abundance ratio seven times greater than expected from the photospheric abundance. Such a large abundance anomaly is unprecedented in the solar atmosphere. We interpreted this result as being due to an inverse first ionization potential (FIP) effect. In the published work, two lines of Ar xiv were observed, and one line was tentatively identified as anmore » Ar xi line. In this paper, we report observing a similar enhancement in a full-CCD EIS flare spectrum in 13 argon lines that lie within the EIS wavelength ranges. The observed lines include two Ar xi lines, four Ar xiii lines, six Ar xiv lines, and one Ar xv line. The enhancement is far less than reported in Doschek et al. but exhibits similar morphology. The argon abundance is close to a photospheric abundance in the enhanced area, and the abundance could be photospheric. This enhancement occurs in association with a sunspot in a small area only a few arcseconds (1″ = about 700 km) in size. There is no enhancement effect observed in the normally high-FIP sulfur and oxygen line ratios relative to lines of low-FIP elements available to EIS. Calculations of path lengths in the strongest enhanced area in Doschek et al. indicate a depletion of low-FIP elements.« less

77 FR 60168 - Environmental Impact Statement: San Joaquin and Stanislaus Counties, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-02

... (3.5 miles) in length. The project was anticipated to improve traffic safety and operations by reducing congestion and accidents. Since the NOI to prepare an EIS was published in the Federal Register on...

Study of supported bilayer lipid membranes for use in chemo-electric energy conversion via active proton transport

NASA Astrophysics Data System (ADS)

Sarles, Stephen A.; Sundaresan, Vishnu B.; Leo, Donald J.

2007-09-01

Bilayer lipid membranes (BLMs) have been studied extensively due to functional and structural similarities to cell membranes, fostering research to understand ion-channel protein functions, measure bilayer mechanical properties, and identify self-assembly mechanisms. BLMs have traditionally been formed across single pores in substrates such as PTFE (Teflon). The incorporation of ion-channel proteins into the lipid bilayer enables the selective transfer of ions and fluid through the BLM. Processes of this nature have led to the measurement of ion current flowing across the lipid membrane and have been used to develop sensors that signal the presence of a particular reactant (glucose, urea, penicillin), improve drug recognition in cells, and develop materials capable of creating chemical energy from light. Recent research at Virginia Tech has shown that the incorporation of proton transporters in a supported BLM formed across an array of pores can convert chemical energy available in the adenosine triphosphate (ATP) into electricity. Experimental results from this work show that the system-named Biocell-is capable of developing 2µW/cm2 of membrane area with 15μl of ATPase. Efforts to increase the power output and conversion efficiency of this process while moving toward a packaged device present a unique engineering problem. The bilayer, as host to the active proton transporters, must therefore be formed evenly across a porous substrate, remain stable and yet fluid-like for protein interaction, and exhibit a large seal resistance. This article presents the ongoing work to characterize the Biocell using impedance analysis. Electrical impedance spectroscopy (EIS) is used to study the effect of adding ATPase proteins to POPS:POPE bilayer lipid membranes and correlate structural changes evident in the impedance data to the energy-conversion capability of various partial and whole Biocell assemblies. The specific membrane resistance of a pure BLM drops from 40-120kΩ•cm2 to only a few hundred Ω•cm2 upon reconstitution of ATPase proteins. Power characterization indicates that ATP hydrolysis may result in charging of the silver-silver chloride electrodes.

Novel multipotent tacrine-dihydropyridine hybrids with improved acetylcholinesterase inhibitory and neuroprotective activities as potential drugs for the treatment of Alzheimer's disease.

PubMed

Marco-Contelles, José; León, Rafael; de Los Ríos, Cristóbal; Guglietta, Antonio; Terencio, José; López, Manuela G; García, Antonio G; Villarroya, Mercedes

2006-12-28

In this work we describe the synthesis and biological evaluation of the tacrine-1,4-dihydropyridine (DHP) hybrids (3-11). These multipotent molecules are the result of the juxtaposition of an acetylcholinesterase inhibitor (AChEI) such as tacrine (1) and a 1,4-DHP such as nimodipine (2). Compounds 3-11 are very selective and potent AChEIs and show an excellent neuroprotective profile and a moderate Ca2+ channel blockade effect. Consequently, these molecules are new potential drugs for the treatment of Alzheimer's disease.

The disaccharide moiety of bleomycin facilitates uptake by cancer cells.

PubMed

Schroeder, Benjamin R; Ghare, M Imran; Bhattacharya, Chandrabali; Paul, Rakesh; Yu, Zhiqiang; Zaleski, Paul A; Bozeman, Trevor C; Rishel, Michael J; Hecht, Sidney M

2014-10-01

The disaccharide moiety is responsible for the tumor cell targeting properties of bleomycin (BLM). While the aglycon (deglycobleomycin) mediates DNA cleavage in much the same fashion as bleomycin, it exhibits diminished cytotoxicity in comparison to BLM. These findings suggested that BLM might be modular in nature, composed of tumor-seeking and tumoricidal domains. To explore this possibility, BLM analogues were prepared in which the disaccharide moiety was attached to deglycobleomycin at novel positions, namely, via the threonine moiety or C-terminal substituent. The analogues were compared with BLM and deglycoBLM for DNA cleavage, cancer cell uptake, and cytotoxic activity. BLM is more potent than deglycoBLM in supercoiled plasmid DNA relaxation, while the analogue having the disaccharide on threonine was less active than deglycoBLM and the analogue containing the C-terminal disaccharide was slightly more potent. While having unexceptional DNA cleavage potencies, both glycosylated analogues were more cytotoxic to cultured DU145 prostate cancer cells than deglycoBLM. Dye-labeled conjugates of the cytotoxic BLM aglycons were used in imaging experiments to determine the extent of cell uptake. The rank order of internalization efficiencies was the same as their order of cytotoxicities toward DU145 cells. These findings establish a role for the BLM disaccharide in tumor targeting/uptake and suggest that the disaccharide moiety may be capable of delivering other cytotoxins to cancer cells. While the mechanism responsible for uptake of the BLM disaccharide selectively by tumor cells has not yet been established, data are presented which suggest that the metabolic shift to glycolysis in cancer cells may provide the vehicle for selective internalization.

43 CFR 2806.32 - How does BLM determine the population strata served?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How does BLM determine the population... does BLM determine the population strata served? (a) BLM determines the population strata served as follows: (1) If the site or facility is within a designated RMA, BLM will use the population strata of the...

43 CFR 2806.32 - How does BLM determine the population strata served?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How does BLM determine the population... does BLM determine the population strata served? (a) BLM determines the population strata served as follows: (1) If the site or facility is within a designated RMA, BLM will use the population strata of the...

43 CFR 2806.32 - How does BLM determine the population strata served?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How does BLM determine the population... does BLM determine the population strata served? (a) BLM determines the population strata served as follows: (1) If the site or facility is within a designated RMA, BLM will use the population strata of the...

43 CFR 2806.32 - How does BLM determine the population strata served?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How does BLM determine the population... does BLM determine the population strata served? (a) BLM determines the population strata served as follows: (1) If the site or facility is within a designated RMA, BLM will use the population strata of the...

77 FR 23740 - Sears Point Wetland and Watershed Restoration Project, Sonoma County, CA; Final Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-20

...We, the U.S. Fish and Wildlife Service (Service) and the California Department of Fish and Game (CDFG), in cooperation with the Sonoma Land Trust (SLT), announce that a final environmental impact report and environmental impact statement (EIR/EIS) for the Sears Point Wetland and Watershed Restoration Project is now available. The final EIR/EIS, which we prepared and now announce in accordance with the National Environmental Policy Act of 1969 (NEPA), describes the restoration of approximately 2,300 acres (ac) of former farmland located in Sonoma County, California, near the San Pablo Bay. The final EIR/EIS responds to all comments we received on the draft document. The restoration project, which would be implemented by the SLT, would restore natural estuarine ecosystems on diked baylands, while providing public access and recreational and educational opportunities compatible with ecological and cultural resources protection. The U.S. Army Corps of Engineers, San Francisco District, and the National Marine Fisheries Service of the National Oceanic and Atmospheric Administration are cooperating agencies on the final EIR/EIS.

Airway transplantation of adipose stem cells protects against bleomycin-induced pulmonary fibrosis.

PubMed

Llontop, Pedro; Lopez-Fernandez, Daniel; Clavo, Bernardino; Afonso Martín, Juan Luis; Fiuza-Pérez, María D; García Arranz, Mariano; Calatayud, Joaquín; Molins López-Rodó, Laureano; Alshehri, Khalid; Ayub, Adil; Raad, Wissam; Bhora, Faiz; Santana-Rodríguez, Norberto

2018-04-01

Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease with poor prognosis. Adipose-derived stem cells (ADSC) have demonstrated regenerative properties in several tissues. The hypothesis of this study was that airway transplantation of ADSC could protect against bleomycin (BLM)-induced pulmonary fibrosis (PF). Fifty-eight lungs from 29 male Sprague-Dawley rats were analyzed. Animals were randomly divided into five groups: a) control (n=3); b) sham (n=6); c) BLM (n=6); d) BLM+ADSC-2d (n=6); and e) BLM+ADSC-14d (n=8). Animals received 500 µL saline (sham), 2.5 UI/kg BLM in 500 µL saline (BLM), and 2×10 6  ADSC in 100 µL saline intratracheally at 2 (BLM+ADSC-2d) and 14 days (BLM+ADSC-14d) after BLM. Animals were sacrificed at 28 days. Blinded Ashcroft score was used to determine pulmonary fibrosis extent on histology. Hsp27, Vegf, Nfkβ, IL-1, IL-6, Col4, and Tgfβ1 mRNA gene expression were determined using real-time quantitative-PCR. Ashcroft index was: control=0; sham=0.37±0.07; BLM=6.55±0.34 vs sham (P=0.006). BLM vs BLM+ADSC-2d=4.63±0.38 (P=0.005) and BLM+ADSC-14d=3.77±0.46 (P=0.005). BLM vs sham significantly increased Hsp27 (P=0.018), Nfkβ (P=0.009), Col4 (P=0.004), Tgfβ1 (P=0.006) and decreased IL-1 (P=0.006). BLM+ADSC-2d vs BLM significantly decreased Hsp27 (P=0.009) and increased Vegf (P=0.006), Nfkβ (P=0.009). BLM+ADSC-14d vs BLM significantly decreased Hsp27 (P=0.028), IL-6 (P=0.013), Col4 (P=0.002), and increased Nfkβ (P=0.040) and Tgfβ1 (P=0.002). Airway transplantation of ADSC significantly decreased the fibrosis rate in both early and established pulmonary fibrosis, modulating the expression of Hsp27, Vegfa, Nfkβ, IL-6, Col4, and Tgfβ1. From a translational perspective, this technique could become a new adjuvant treatment for patients with IPF. © American Federation for Medical Research (unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

Summary and findings of the 2006 BLM Forest Lands Report

Treesearch

Tim Bottomley; Jim Menlove

2009-01-01

In 2006, the Bureau of Land Management (BLM) contracted with the Forest Service Forest Inventory and Analysis Program (FIA) to assist in the preparation of a report specific to all forest lands under the administration of the BLM. The BLM requested that the FIA provide information on the extent and general conditions of BLM- managed forests and woodlands, within...

346. Caltrans, Photographer July 8, 1935 "PIER El"; VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

346. Caltrans, Photographer July 8, 1935 "PIER E-l"; VIEW OF PIER E-I, DECK TRUSS, AND CANTILEVER TRUSS ANCHOR ARM UNDER CONSTRUCTION. 5-1583 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

The DNA Helicase Activity of BLM Is Necessary for the Correction of the Genomic Instability of Bloom Syndrome Cells

PubMed Central

Neff, Norma F.; Ellis, Nathan A.; Ye, Tian Zhang; Noonan, James; Huang, Kelly; Sanz, Maureen; Proytcheva, Maria

1999-01-01

Bloom syndrome (BS) is a rare autosomal recessive disorder characterized by growth deficiency, immunodeficiency, genomic instability, and the early development of cancers of many types. BLM, the protein encoded by BLM, the gene mutated in BS, is localized in nuclear foci and absent from BS cells. BLM encodes a DNA helicase, and proteins from three missense alleles lack displacement activity. BLM transfected into BS cells reduces the frequency of sister chromatid exchanges and restores BLM in the nucleus. Missense alleles fail to reduce the sister chromatid exchanges in transfected BS cells or restore the normal nuclear pattern. BLM complements a phenotype of a Saccharomyces cerevisiae sgs1 top3 strain, and the missense alleles do not. This work demonstrates the importance of the enzymatic activity of BLM for its function and nuclear localization pattern. PMID:10069810

BLM promotes the activation of Fanconi Anemia signaling pathway.

PubMed

Panneerselvam, Jayabal; Wang, Hong; Zhang, Jun; Che, Raymond; Yu, Herbert; Fei, Peiwen

2016-05-31

Mutations in the human RecQ helicase, BLM, causes Bloom Syndrome, which is a rare autosomal recessive disorder and characterized by genomic instability and an increased risk of cancer. Fanconi Anemia (FA), resulting from mutations in any of the 19 known FA genes and those yet to be known, is also characterized by chromosomal instability and a high incidence of cancer. BLM helicase and FA proteins, therefore, may work in a common tumor-suppressor signaling pathway. To date, it remains largely unclear as to how BLM and FA proteins work concurrently in the maintenance of genome stability. Here we report that BLM is involved in the early activation of FA group D2 protein (FANCD2). We found that FANCD2 activation is substantially delayed and attenuated in crosslinking agent-treated cells harboring deficient Blm compared to similarly treated control cells with sufficient BLM. We also identified that the domain VI of BLM plays an essential role in promoting FANCD2 activation in cells treated with DNA crosslinking agents, especially ultraviolet B. The similar biological effects performed by ΔVI-BLM and inactivated FANCD2 further confirm the relationship between BLM and FANCD2. Mutations within the domain VI of BLM detected in human cancer samples demonstrate the functional importance of this domain, suggesting human tumorigenicity resulting from mtBLM may be at least partly attributed to mitigated FANCD2 activation. Collectively, our data show a previously unknown regulatory liaison in advancing our understanding of how the cancer susceptibility gene products act in concert to maintain genome stability.

Copper toxicity and organic matter: Resiliency of watersheds in the Duluth Complex, Minnesota, USA

USGS Publications Warehouse

Piatak, Nadine; Seal, Robert; Jones, Perry M.; Woodruff, Laurel G.

2015-01-01

We estimated copper (Cu) toxicity in surface water with high dissolved organic matter (DOM) for unmined mineralized watersheds of the Duluth Complex using the Biotic Ligand Model (BLM), which evaluates the effect of DOM, cation competition for biologic binding sites, and metal speciation. A sediment-based BLM was used to estimate stream-sediment toxicity; this approach factors in the cumulative effects of multiple metals, incorporation of metals into less bioavailable sulfides, and complexation of metals with organic carbon. For surface water, the formation of Cu-DOM complexes significantly reduces the amount of Cu available to aquatic organisms. The protective effects of cations, such as calcium (Ca) and magnesium (Mg), competing with Cu to complex with the biotic ligand is likely not as important as DOM in water with high DOM and low hardness. Standard hardness-based water quality criteria (WQC) are probably inadequate for describing Cu toxicity in such waters and a BLM approach may yield more accurate results. Nevertheless, assumptions about relative proportions of humic acid (HA) and fulvic acid (FA) in DOM significantly influence BLM results; the higher the HA fraction, the higher calculated resiliency of the water to Cu toxicity. Another important factor is seasonal variation in water chemistry, with greater resiliency to Cu toxicity during low flow compared to high flow.Based on generally low total organic carbon and sulfur content, and equivalent metal ratios from total and weak partial extractions, much of the total metal concentration in clastic streambedsediments may be in bioavailable forms, sorbed on clays or hydroxide phases. However, organicrich fine-grained sediment in the numerous wetlands may sequester significant amount of metals, limiting their bioavailability. A high proportion of organic matter in waters and some sediments will play a key role in the resiliency of these watersheds to potential additional metal loads associated with future mining operations.

Blm10 facilitates nuclear import of proteasome core particles

PubMed Central

Weberruss, Marion H; Savulescu, Anca F; Jando, Julia; Bissinger, Thomas; Harel, Amnon; Glickman, Michael H; Enenkel, Cordula

2013-01-01

Short-lived proteins are degraded by proteasome complexes, which contain a proteolytic core particle (CP) but differ in the number of regulatory particles (RPs) and activators. A recently described member of conserved proteasome activators is Blm10. Blm10 contains 32 HEAT-like modules and is structurally related to the nuclear import receptor importin/karyopherin β. In proliferating yeast, RP-CP assemblies are primarily nuclear and promote cell division. During quiescence, RP-CP assemblies dissociate and CP and RP are sequestered into motile cytosolic proteasome storage granuli (PSG). Here, we show that CP sequestration into PSG depends on Blm10, whereas RP sequestration into PSG is independent of Blm10. PSG rapidly clear upon the resumption of cell proliferation and proteasomes are relocated into the nucleus. Thereby, Blm10 facilitates nuclear import of CP. Blm10-bound CP serves as an import receptor–cargo complex, as Blm10 mediates the interaction with FG-rich nucleoporins and is dissociated from the CP by Ran-GTP. Thus, Blm10 represents the first CP-dedicated nuclear import receptor in yeast. PMID:23982732

Loss of a 20S Proteasome Activator in Saccharomyces cerevisiae Downregulates Genes Important for Genomic Integrity, Increases DNA Damage, and Selectively Sensitizes Cells to Agents With Diverse Mechanisms of Action

PubMed Central

Doherty, Kevin M.; Pride, Leah D.; Lukose, James; Snydsman, Brian E.; Charles, Ronald; Pramanik, Ajay; Muller, Eric G.; Botstein, David; Moore, Carol Wood

2012-01-01

Cytoprotective functions of a 20S proteasome activator were investigated. Saccharomyces cerevisiae Blm10 and human 20S proteasome activator 200 (PA200) are homologs. Comparative genome-wide analyses of untreated diploid cells lacking Blm10 and growing at steady state at defined growth rates revealed downregulation of numerous genes required for accurate chromosome structure, assembly and repair, and upregulation of a specific subset of genes encoding protein-folding chaperones. Blm10 loss or truncation of the Ubp3/Blm3 deubiquitinating enzyme caused massive chromosomal damage and cell death in homozygous diploids after phleomycin treatments, indicating that Blm10 and Ubp3/Blm3 function to stabilize the genome and protect against cell death. Diploids lacking Blm10 also were sensitized to doxorubicin, hydroxyurea, 5-fluorouracil, rapamycin, hydrogen peroxide, methyl methanesulfonate, and calcofluor. Fluorescently tagged Blm10 localized in nuclei, with enhanced fluorescence after DNA replication. After DNA damage that caused a classic G2/M arrest, fluorescence remained diffuse, with evidence of nuclear fragmentation in some cells. Protective functions of Blm10 did not require the carboxyl-terminal region that makes close contact with 20S proteasomes, indicating that protection does not require this contact or the truncated Blm10 can interact with the proteasome apart from this region. Without its carboxyl-terminus, Blm10(−339aa) localized to nuclei in untreated, nonproliferating (G0) cells, but not during G1 S, G2, and M. The results indicate Blm10 functions in protective mechanisms that include the machinery that assures proper assembly of chromosomes. These essential guardian functions have implications for ubiquitin-independent targeting in anticancer therapy. Targeting Blm10/PA200 together with one or more of the upregulated chaperones or a conventional treatment could be efficacious. PMID:22908043

PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution

PubMed Central

Ke, Yuwen; Huh, Jae-Wan; Warrington, Ross; Li, Bing; Wu, Nan; Leng, Mei; Zhang, Junmei; Ball, Haydn L; Li, Bing; Yu, Hongtao

2011-01-01

Centromeres nucleate the formation of kinetochores and are vital for chromosome segregation during mitosis. The SNF2 family helicase PICH (Plk1-interacting checkpoint helicase) and the BLM (the Bloom's syndrome protein) helicase decorate ultrafine histone-negative DNA threads that link the segregating sister centromeres during anaphase. The functions of PICH and BLM at these threads are not understood, however. Here, we show that PICH binds to BLM and enables BLM localization to anaphase centromeric threads. PICH- or BLM-RNAi cells fail to resolve these threads in anaphase. The fragmented threads form centromeric-chromatin-containing micronuclei in daughter cells. Anaphase threads in PICH- and BLM-RNAi cells contain histones and centromere markers. Recombinant purified PICH has nucleosome remodelling activities in vitro. We propose that PICH and BLM unravel centromeric chromatin and keep anaphase DNA threads mostly free of nucleosomes, thus allowing these threads to span long distances between rapidly segregating centromeres without breakage and providing a spatiotemporal window for their resolution. PMID:21743438

DISTANT VIEW, BLM TACK SHED ON LEFT, BLM SEED SHED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DISTANT VIEW, BLM TACK SHED ON LEFT, BLM SEED SHED AT LEFT CENTER, FIRE DISPATCH OFFICES 1 AND 2 AT RIGHT CENTER, UTILITY BUILDING "B" ON RIGHT. VIEW TO SOUTHWEST. - Cedar City Automotive Repair Shop, 820 North Main Street, Cedar City, Iron County, UT

76 FR 2878 - Six Rivers National Forest, Mad River Ranger District, CA; Buck Mountain Vegetation and Fuel...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-18

...; Buck Mountain Vegetation and Fuel Management Project EIS AGENCY: Forest Service, USDA. ACTION: Notice... the Buck Mountain Vegetation and Fuel Management Project to accelerate late-successional forest... project design features have been identified. Late-Successional Reserve Management Silvicultural...

77 FR 76067 - Notice of Availability of a Proposed Land Use Plan Amendment and Final Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-26

... Statement for the Proposed McCoy Solar Energy Project, CA AGENCY: Bureau of Land Management, Interior... amendment and final environmental impact statement (EIS) for the McCoy Solar Energy Project (project)--a photovoltaic solar electricity generation [[Page 76068

Effect of DC Plasma Electrolytic Oxidation on Surface Characteristics and Corrosion Resistance of Zirconium

PubMed Central

Sowa, Maciej

2018-01-01

Zr is a valve metal, the biocompatibility of which is at least on par with Ti. Recently, numerous attempts of the formation of bioactive coatings on Zr by plasma electrolytic oxidation (PEO) in solutions that were based on calcium acetate and calcium β-glycerophosphate were made. In this study, the direct current (DC) PEO of commercially pure zirconium in the solutions that contained Ca(H2PO2)2, Ca(HCOO)2, and Mg(CH3COO)2 was investigated. The treatment was conducted at 75 mA/cm2 up to 200, 300, or 400 V. Five process stages were discerned. The treatment at higher voltages resulted in the formation of oxide layers that had Ca/P or (Mg+Ca)/P ratios that were close to that of hydroxyapatite (Ca/P = 1.67), determined by SEM/EDX. The corrosion resistance studies were performed using electrochemical impedance spectroscopy (EIS) and DC polarization methods. R(Q[R(QR)]) circuit model was used to fit the EIS data. In general, the coatings that were obtained at 200 V were the most corrosion resistant, however, they lacked the porous structure, which is typical for PEO coatings, and is sought after in the biomedical applications. The treatment at 400 V resulted in the formation of the coatings that were more corrosion resistant than those that were obtained at 300 V. This was determined mainly by the prevailing plasma regime at the given process voltage. The pitting resistance of Zr was also improved by the treatment, regardless of the applied process conditions. PMID:29751530

BLM and RMI1 alleviate RPA inhibition of TopoIIIα decatenase activity.

PubMed

Yang, Jay; Bachrati, Csanad Z; Hickson, Ian D; Brown, Grant W

2012-01-01

RPA is a single-stranded DNA binding protein that physically associates with the BLM complex. RPA stimulates BLM helicase activity as well as the double Holliday junction dissolution activity of the BLM-topoisomerase IIIα complex. We investigated the effect of RPA on the ssDNA decatenase activity of topoisomerase IIIα. We found that RPA and other ssDNA binding proteins inhibit decatenation by topoisomerase IIIα. Complex formation between BLM, TopoIIIα, and RMI1 ablates inhibition of decatenation by ssDNA binding proteins. Together, these data indicate that inhibition by RPA does not involve species-specific interactions between RPA and BLM-TopoIIIα-RMI1, which contrasts with RPA modulation of double Holliday junction dissolution. We propose that topoisomerase IIIα and RPA compete to bind to single-stranded regions of catenanes. Interactions with BLM and RMI1 enhance toposiomerase IIIα activity, promoting decatenation in the presence of RPA.

43 CFR 2804.19 - How will BLM process my Processing Category 6 application?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How will BLM process my Processing... process my Processing Category 6 application? (a) For Processing Category 6 applications, you and BLM must enter into a written agreement that describes how BLM will process your application. The final agreement...

43 CFR 2804.19 - How will BLM process my Processing Category 6 application?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How will BLM process my Processing... process my Processing Category 6 application? (a) For Processing Category 6 applications, you and BLM must enter into a written agreement that describes how BLM will process your application. The final agreement...

Up-regulation of antioxidant enzymes and coenzyme Q(10) in a human oral cancer cell line with acquired bleomycin resistance.

PubMed

Yen, Hsiu-Chuan; Li, Sin-Hua; Majima, Hideyuki J; Huang, Yu-Hsiang; Chen, Chiu-Ping; Liu, Chia-Chi; Tu, Ya-Chi; Chen, Chih-Wei

2011-06-01

Bleomycin (BLM) is an anti-cancer drug that can induce formation of reactive oxygen species (ROS). To investigate the association between up-regulation of antioxidant enzymes and coenzyme Q(10) (CoQ(10)) in acquired BLM resistance, one BLM-resistant clone, SBLM24 clone, was selected from a human oral cancer cell line, SCC61 clone. The BLM resistance of SBLM24 clone relative to a sub-clone of SCC61b cells was confirmed by analysis of clonogenic ability and cell cycle arrest. CoQ(10) levels and levels of Mn superoxide dismutase, glutathione peroxidase 1, catalase and thioredoxin reductase 1 were augmented in SBLM24 clone although there was also a mild increase in the expression of BLM hydrolase. Suppression of CoQ(10) levels by 4-aminobenzoate sensitized BLM-induced cytotoxicity. The results of suppression on enhanced ROS production by BLM and the cross-resistance to hydrogen peroxide in SBLM24 clone further demonstrated the development of adaptation to oxidative stress during the formation of acquired BLM resistance.

Interaction between the helicases genetically linked to Fanconi anemia group J and Bloom's syndrome

PubMed Central

Suhasini, Avvaru N; Rawtani, Nina A; Wu, Yuliang; Sommers, Joshua A; Sharma, Sudha; Mosedale, Georgina; North, Phillip S; Cantor, Sharon B; Hickson, Ian D; Brosh, Robert M

2011-01-01

Bloom's syndrome (BS) and Fanconi anemia (FA) are autosomal recessive disorders characterized by cancer and chromosomal instability. BS and FA group J arise from mutations in the BLM and FANCJ genes, respectively, which encode DNA helicases. In this work, FANCJ and BLM were found to interact physically and functionally in human cells and co-localize to nuclear foci in response to replication stress. The cellular level of BLM is strongly dependent upon FANCJ, and BLM is degraded by a proteasome-mediated pathway when FANCJ is depleted. FANCJ-deficient cells display increased sister chromatid exchange and sensitivity to replication stress. Expression of a FANCJ C-terminal fragment that interacts with BLM exerted a dominant negative effect on hydroxyurea resistance by interfering with the FANCJ–BLM interaction. FANCJ and BLM synergistically unwound a DNA duplex substrate with sugar phosphate backbone discontinuity, but not an ‘undamaged' duplex. Collectively, the results suggest that FANCJ catalytic activity and its effect on BLM protein stability contribute to preservation of genomic stability and a normal response to replication stress. PMID:21240188

HEALTH AND NUTRITIONAL ASSESSMENT OF FREE-RANGING EASTERN INDIGO SNAKES (DRYMARCHON COUPERI) IN GEORGIA, UNITED STATES.

PubMed

Knafo, S Emmanuelle; Norton, Terry M; Mitchell, Mark; Stevenson, Dirk J; Hyslop, Natalie; Poppenga, Robert; Oliva, Marcie; Chen, Tai; Cray, Carolyn; Gibbs, Samantha E J; Durden, Lance; Stedman, Nancy; Divers, Stephen; Dierenfeld, Ellen

2016-12-01

Clinical pathology and nutritional parameters are useful in evaluating and monitoring threatened and endangered wildlife populations, but reference ranges for most snake species are lacking. From 2001 to 2005, health assessments were performed on 58 eastern indigo snakes (EIS) (Drymarchon couperi) captured in the wild in southeastern Georgia, United States. Health and nutritional assessments performed included hematology, serum biochemistry, fat-soluble vitamins, heavy metals, pesticide contaminants, parasitology, and surveys of other pathogens. Significant differences in total solids, packed cell volume, glucose, blood urea nitrogen, albumin : globulin ratio, amylase, triglycerides, and bile acids between males and females were observed. Additionally, there was a significant difference between liver and kidney concentrations for vitamins A and E. As previously noted in captive EIS, total Ca was elevated in comparison to concentrations reported in other snake species. Parasitism was a common finding in sampled EIS, but the overall health status of this free-ranging population appeared good. A winter-time dermatitis was found in most snakes, which resolved in the summer months. This study represents the first health and nutritional assessment of free-ranging EIS, and provides needed data to guide monitoring and conservation efforts.

77 FR 68813 - Notice of Availability of the Draft Environmental Impact Statement and Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-16

... geothermal unit, which is currently providing energy sufficient to power three operating geothermal plants... the Casa Diablo IV Geothermal Development Project, CA AGENCY: Bureau of Land Management, Interior... Statement (EIS)/Environmental Impact Report (EIR) for the proposed Casa Diablo IV Geothermal Development...

76 FR 55703 - Draft Environmental Impact Statement/Environmental Impact Report and Notice of Public Meetings...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-08

... DEPARTMENT OF THE INTERIOR Bureau of Reclamation Draft Environmental Impact Statement..., Sacramento County, CA AGENCY: Bureau of Reclamation, Interior. ACTION: Notice of availability and public.... The preliminary design concepts being considered in the Draft EIS/EIR are one three-story building or...

78 FR 65643 - Environmental Impact Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-01

..., Gas Hills In-Situ Recovery Uranium Project, Review Period Ends: 12/02/2013, Contact: Tom Sunderland..., Tiering FEIS--U.S. Coast Guard Rulemaking for Dry Cargo Residue Discharges in the Great Lakes, Review... No. 20130290, Draft EIS, NPS, CA, Restoration of Native Species in High Elevation Aquatic Ecosystems...

Predicting toxic effects of copper on aquatic biota in mineralized areas by using the Biotic Ligand Model

USGS Publications Warehouse

Smith, Kathleen S.; Ranville, James F.; Adams, M.; Choate, LaDonna M.; Church, Stan E.; Fey, David L.; Wanty, Richard B.; Crock, James G.

2006-01-01

The chemical speciation of metals influences their biological effects. The Biotic Ligand Model (BLM) is a computational approach to predict chemical speciation and acute toxicological effects of metals on aquatic biota. Recently, the U.S. Environmental Protection Agency incorporated the BLM into their regulatory water-quality criteria for copper. Results from three different laboratory copper toxicity tests were compared with BLM predictions for simulated test-waters. This was done to evaluate the ability of the BLM to accurately predict the effects of hardness and concentrations of dissolved organic carbon (DOC) and iron on aquatic toxicity. In addition, we evaluated whether the BLM and the three toxicity tests provide consistent results. Comparison of BLM predictions with two types of Ceriodaphnia dubia toxicity tests shows that there is fairly good agreement between predicted LC50 values computed by the BLM and LC50 values determined from the two toxicity tests. Specifically, the effect of increasing calcium concentration (and hardness) on copper toxicity appears to be minimal. Also, there is fairly good agreement between the BLM and the two toxicity tests for test solutions containing elevated DOC, for which the LC50 is 3-to-5 times greater (less toxic) than the LC50 for the lower-DOC test water. This illustrates the protective effects of DOC on copper toxicity and demonstrates the ability of the BLM to predict these protective effects. In contrast, for test solutions with added iron there is a decrease in LC50 values (increase in toxicity) in results from the two C. dubia toxicity tests, and the agreement between BLM LC50 predictions and results from these toxicity tests is poor. The inability of the BLM to account for competitive iron binding to DOC or DOC fractionation may be a significant shortcoming of the BLM for predicting site- specific water-quality criteria in streams affected by iron-rich acidic drainage in mined and mineralized areas.

43 CFR 2885.15 - How will BLM charge me rent?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How will BLM charge me rent? 2885.15 Section 2885.15 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND... LEASING ACT Terms and Conditions of MLA Grants and TUPs § 2885.15 How will BLM charge me rent? (a) BLM...

43 CFR 2806.11 - How will BLM charge me rent?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How will BLM charge me rent? 2806.11 Section 2806.11 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND... POLICY MANAGEMENT ACT Rents General Provisions § 2806.11 How will BLM charge me rent? (a) BLM will charge...

43 CFR 2806.11 - How will BLM charge me rent?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How will BLM charge me rent? 2806.11 Section 2806.11 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND... POLICY MANAGEMENT ACT Rents General Provisions § 2806.11 How will BLM charge me rent? (a) BLM will charge...

43 CFR 2885.15 - How will BLM charge me rent?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How will BLM charge me rent? 2885.15 Section 2885.15 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND... LEASING ACT Terms and Conditions of MLA Grants and TUPs § 2885.15 How will BLM charge me rent? (a) BLM...

43 CFR 3203.14 - How will BLM provide notice of a competitive lease sale?

Code of Federal Regulations, 2011 CFR

2011-10-01

... before conducting a competitive lease sale, BLM will post the Notice in the BLM office having... having jurisdiction over any of the included lands. (c) BLM may take other measures of notification for... sale; (3) Publishing notice in the newspaper; or (4) Posting the list of parcels on the Internet. ...

43 CFR 3217.14 - When will BLM approve my drilling or development contract?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false When will BLM approve my drilling or... RESOURCE LEASING Cooperative Agreements § 3217.14 When will BLM approve my drilling or development contract? BLM may approve a drilling or development contract when: (a) One or more geothermal lessees enter into...

43 CFR 3217.14 - When will BLM approve my drilling or development contract?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false When will BLM approve my drilling or... RESOURCE LEASING Cooperative Agreements § 3217.14 When will BLM approve my drilling or development contract? BLM may approve a drilling or development contract when: (a) One or more geothermal lessees enter into...

76 FR 66958 - Notice of Availability of the Supplement to the Draft Programmatic Environmental Impact Statement...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-28

... energy development on BLM-administered lands. The elements of the BLM's proposed Solar Energy Program.... Amendment of BLM land use plans in the six-state study area to adopt those elements of the new Solar Energy... development, the BLM also proposes a process that will accommodate responsible development outside of SEZs...

43 CFR 2884.17 - How will BLM process my Processing Category 6 application?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How will BLM process my Processing...-WAY UNDER THE MINERAL LEASING ACT Applying for MLA Grants or TUPs § 2884.17 How will BLM process my... written agreement that describes how BLM will process your application. The final agreement consists of a...

43 CFR 2884.17 - How will BLM process my Processing Category 6 application?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How will BLM process my Processing...-WAY UNDER THE MINERAL LEASING ACT Applying for MLA Grants or TUPs § 2884.17 How will BLM process my... written agreement that describes how BLM will process your application. The final agreement consists of a...

Somatic frameshift mutations in the Bloom syndrome BLM gene are frequent in sporadic gastric carcinomas with microsatellite mutator phenotype

PubMed Central

Calin, George; Ranzani, Guglielmina N; Amadori, Dino; Herlea, Vlad; Matei, Irina; Barbanti-Brodano, Giuseppe; Negrini, Massimo

2001-01-01

Background Genomic instability has been reported at microsatellite tracts in few coding sequences. We have shown that the Bloom syndrome BLM gene may be a target of microsatelliteinstability (MSI) in a short poly-adenine repeat located in its coding region. To further characterize the involvement of BLM in tumorigenesis, we have investigated mutations in nine genes containing coding microsatellites in microsatellite mutator phenotype (MMP) positive and negative gastric carcinomas (GCs). Methods We analyzed 50 gastric carcinomas (GCs) for mutations in the BLM poly(A) tract aswell as in the coding microsatellites of the TGFβ1-RII, IGFIIR, hMSH3, hMSH6, BAX, WRN, RECQL and CBL genes. Results BLM mutations were found in 27% of MMP+ GCs (4/15 cases) but not in any of the MMP negative GCs (0/35 cases). The frequency of mutations in the other eight coding regions microsatellite was the following: TGFβ1-RII (60 %), BAX (27%), hMSH6 (20%),hMSH3 (13%), CBL (13%), IGFIIR (7%), RECQL (0%) and WRN (0%). Mutations in BLM appear to be more frequently associated with frameshifts in BAX and in hMSH6and/or hMSH3. Tumors with BLM alterations present a higher frequency of unstable mono- and trinucleotide repeats located in coding regions as compared with mutator phenotype tumors without BLM frameshifts. Conclusions BLM frameshifts are frequent alterations in GCs specifically associated with MMP+tumors. We suggest that BLM loss of function by MSI may increase the genetic instability of a pre-existent unstable genotype in gastric tumors. PMID:11532193

Telomere and ribosomal DNA repeats are chromosomal targets of the bloom syndrome DNA helicase

PubMed Central

Schawalder, James; Paric, Enesa; Neff, Norma F

2003-01-01

Background Bloom syndrome is one of the most cancer-predisposing disorders and is characterized by genomic instability and a high frequency of sister chromatid exchange. The disorder is caused by loss of function of a 3' to 5' RecQ DNA helicase, BLM. The exact role of BLM in maintaining genomic integrity is not known but the helicase has been found to associate with several DNA repair complexes and some DNA replication foci. Results Chromatin immunoprecipitation of BLM complexes recovered telomere and ribosomal DNA repeats. The N-terminus of BLM, required for NB localization, is the same as the telomere association domain of BLM. The C-terminus is required for ribosomal DNA localization. BLM localizes primarily to the non-transcribed spacer region of the ribosomal DNA repeat where replication forks initiate. Bloom syndrome cells expressing the deletion alleles lacking the ribosomal DNA and telomere association domains have altered cell cycle populations with increased S or G2/M cells relative to normal. Conclusion These results identify telomere and ribosomal DNA repeated sequence elements as chromosomal targets for the BLM DNA helicase during the S/G2 phase of the cell cycle. BLM is localized in nuclear bodies when it associates with telomeric repeats in both telomerase positive and negative cells. The BLM DNA helicase participates in genomic stability at ribosomal DNA repeats and telomeres. PMID:14577841

Intermittent Hypoxia Increases the Severity of Bleomycin-Induced Lung Injury in Mice.

PubMed

Gille, Thomas; Didier, Morgane; Rotenberg, Cécile; Delbrel, Eva; Marchant, Dominique; Sutton, Angela; Dard, Nicolas; Haine, Liasmine; Voituron, Nicolas; Bernaudin, Jean-François; Valeyre, Dominique; Nunes, Hilario; Besnard, Valérie; Boncoeur, Emilie; Planès, Carole

2018-01-01

Severe obstructive sleep apnea (OSA) with chronic intermittent hypoxia (IH) is common in idiopathic pulmonary fibrosis (IPF). Here, we evaluated the impact of IH on bleomycin- (BLM-) induced pulmonary fibrosis in mice. C57BL/6J mice received intratracheal BLM or saline and were exposed to IH (40 cycles/hour; FiO 2 nadir: 6%; 8 hours/day) or intermittent air (IA). In the four experimental groups, we evaluated (i) survival; (ii) alveolar inflammation, pulmonary edema, lung oxidative stress, and antioxidant enzymes; (iii) lung cell apoptosis; and (iv) pulmonary fibrosis. Survival at day 21 was lower in the BLM-IH group ( p < 0.05). Pulmonary fibrosis was more severe at day 21 in BLM-IH mice, as assessed by lung collagen content ( p = 0.02) and histology. At day 4, BLM-IH mice developed a more severe neutrophilic alveolitis, ( p < 0.001). Lung oxidative stress was observed, and superoxide dismutase and glutathione peroxidase expression was decreased in BLM-IH mice ( p < 0.05 versus BLM-IA group). At day 8, pulmonary edema was observed and lung cell apoptosis was increased in the BLM-IH group. These results show that exposure to chronic IH increases mortality, lung inflammation, and lung fibrosis in BLM-treated mice. This study raises the question of the worsening impact of severe OSA in IPF patients.

43 CFR 3602.14 - What kind of financial security does BLM require?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false What kind of financial security does BLM...? (a) For contracts of $2,000 or more, BLM will require a performance bond of an amount sufficient to... post a performance bond. (b) BLM may require a performance bond for contracts of less than $2,000. We...

43 CFR 3602.14 - What kind of financial security does BLM require?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false What kind of financial security does BLM...? (a) For contracts of $2,000 or more, BLM will require a performance bond of an amount sufficient to... post a performance bond. (b) BLM may require a performance bond for contracts of less than $2,000. We...

43 CFR 3602.14 - What kind of financial security does BLM require?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false What kind of financial security does BLM...? (a) For contracts of $2,000 or more, BLM will require a performance bond of an amount sufficient to... post a performance bond. (b) BLM may require a performance bond for contracts of less than $2,000. We...

43 CFR 3834.13 - Will BLM prorate annual maintenance or oil shale fees?

Code of Federal Regulations, 2012 CFR

2012-10-01

... FOR MINING CLAIMS OR SITES Fee Payment § 3834.13 Will BLM prorate annual maintenance or oil shale fees? BLM will not prorate annual maintenance or oil shale fees if you hold a mining claim or site for only... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Will BLM prorate annual maintenance or oil...

43 CFR 3834.13 - Will BLM prorate annual maintenance or oil shale fees?

Code of Federal Regulations, 2014 CFR

2014-10-01

... FOR MINING CLAIMS OR SITES Fee Payment § 3834.13 Will BLM prorate annual maintenance or oil shale fees? BLM will not prorate annual maintenance or oil shale fees if you hold a mining claim or site for only... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Will BLM prorate annual maintenance or oil...

43 CFR 3834.13 - Will BLM prorate annual maintenance or oil shale fees?

Code of Federal Regulations, 2013 CFR

2013-10-01

... FOR MINING CLAIMS OR SITES Fee Payment § 3834.13 Will BLM prorate annual maintenance or oil shale fees? BLM will not prorate annual maintenance or oil shale fees if you hold a mining claim or site for only... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Will BLM prorate annual maintenance or oil...

43 CFR 3834.13 - Will BLM prorate annual maintenance or oil shale fees?

Code of Federal Regulations, 2011 CFR

2011-10-01

... FOR MINING CLAIMS OR SITES Fee Payment § 3834.13 Will BLM prorate annual maintenance or oil shale fees? BLM will not prorate annual maintenance or oil shale fees if you hold a mining claim or site for only... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Will BLM prorate annual maintenance or oil...

Assessing the Potential for Renewable Energy on Public Lands

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

2003-02-01

This report represents an initial activity of the Bureau of Land Managements (BLM) proposed National Energy Policy Implementation Plan: identify and evaluate renewable energy resources on federal lands and any limitations on accessing them. Ultimately, BLM will prioritize land-use planning activities to increase industrys development of renewable energy resources. These resources include solar, biomass, geothermal, water, and wind energy. To accomplish this, BLM and the Department of Energys National Renewable Energy Laboratory (NREL) established a partnership to conduct an assessment of renewable energy resources on BLM lands in the western United States. The objective of this collaboration was to identifymore » BLM planning units in the western states with the highest potential for private-sector development of renewable resources. The assessment resulted in the following findings: (1) 63 BLM planning units in nine western states have high potential for one or more renewable energy technologies; and (2) 20 BLM planning units in seven western states have high potential for power production from three or more renewable energy sources. This assessment report provides BLM with information needed to prioritize land-use planning activities on the basis of potential for the development of energy from renewable resources.« less

Influence of inhalation anesthetics on ion transport across a planar bilayer lipid membrane.

PubMed

Hichiri, Kei; Shirai, Osamu; Kano, Kenji

2012-01-01

Ion transport from one aqueous phase (W1) to another (W2) across a planar bilayer lipid membrane (BLM) in the presence of inhalation anesthetics was electrochemically investigated. In the absence of inhalation anesthetics in the BLM system, no ion transport current flowed between W1 and W2 across the BLM. When inhalation anesthetics such as halothane, chloroform, diethyl ether and trichloroethylene were added to the two aqueous phases or the BLM, the ion transport current quite clearly appeared. When the ratio of the concentration of KCl or NaCl in W1 to that in W2 was varied, the zero current potential across the BLM was shifted. By considering the magnitude of the potential shift, we concluded that the ion transport current can be predominantly ascribed to the transport of Cl(-) across the BLM. Since the dielectric constants of these anesthetics are larger than that of the inner hydrophobic domain of the BLM, the concentration of hydrophilic electrolyte ions in the BLM increases with the increase in the dielectric constant of the inner hydrophobic domain caused by addition of these anesthetics. These situations lead to an increase in the ion permeability coefficient.

Implicit and Explicit Racial Attitudes Changed During Black Lives Matter.

PubMed

Sawyer, Jeremy; Gampa, Anup

2018-07-01

Lab-based interventions have been ineffective in changing individuals' implicit racial attitudes for more than brief durations, and exposure to high-status Black exemplars like Obama has proven ineffective in shifting societal-level racial attitudes. Antiracist social movements, however, offer a potential societal-level alternative for reducing racial bias. Racial attitudes were examined before and during Black Lives Matter (BLM) and its high points of struggle with 1,369,204 participants from 2009 to 2016. After controlling for changes in participant demographics, overall implicit attitudes were less pro-White during BLM than pre-BLM, became increasingly less pro-White across BLM, and were less pro-White during most periods of high BLM struggle. Considering changes in implicit attitudes by participant race, Whites became less implicitly pro-White during BLM, whereas Blacks showed little change. Regarding explicit attitudes, Whites became less pro-White and Blacks became less pro-Black during BLM, each moving toward an egalitarian "no preference" position.

Bleomycin in Octaarginine-modified Fusogenic Liposomes Results in Improved Tumor Growth Inhibition

PubMed Central

Koshkaryev, Alexander; Piroyan, Aleksandr; Torchilin, Vladimir P.

2012-01-01

Bleomycin (BLM) is an example of an anticancer drug that should be delivered into cytosol for its efficient therapeutic action. With this in mind, we developed octaarginine (R8)-modified fusogenic DOPE-liposomes (R8-DOPE-BLM). R8-modification dramatically increased (up to 50-fold) the cell-liposome interaction. R8-DOPE-liposomes were internalized via macropinocytosis and did not end up in the lysosomes. R8-DOPE-BLM led to a significantly stronger cell death and DNA damage in vitro relative to all controls. R8-DOPE-BLM demonstrated a prominent anticancer effect in the BALB/c mice bearing 4T1 tumors. Thus, R8-DOPE-BLM provided efficient intracellular delivery of BLM leading to strong tumor growth inhibition in vivo. PMID:22743614

A reusable device for electrochemical applications of hydrogel supported black lipid membranes.

PubMed

Mech-Dorosz, Agnieszka; Heiskanen, Arto; Bäckström, Sania; Perry, Mark; Muhammad, Haseena B; Hélix-Nielsen, Claus; Emnéus, Jenny

2015-02-01

Black lipid membranes (BLMs) are significant in studies of membrane transport, incorporated proteins/ion transporters, and hence in construction of biosensor devices. Although BLMs provide an accepted mimic of cellular membranes, they are inherently fragile. Techniques are developed to stabilize them, such as hydrogel supports. In this paper, we present a reusable device for studies on hydrogel supported (hs) BLMs. These are formed across an ethylene tetrafluoroethylene (ETFE) aperture array supported by the hydrogel, which is during in situ polymerization covalently "sandwiched" between the ETFE substrate and a gold electrode microchip, thus allowing direct electrochemical studies with the integrated working electrodes. Using electrochemical impedance spectroscopy (EIS), X-ray photoelectron spectroscopy and contact angle measurements, we demonstrate the optimized chemical modifications of the gold electrode microchips and plasma modification of the ETFE aperture arrays facilitating covalent "sandwiching" of the hydrogel. Both fluorescence microscopy and EIS were used to demonstrate the induced spontaneous thinning of a deposited lipid solution, leading to formation of stabilized hsBLMs on average in 10 min. The determined specific membrane capacitance and resistance were shown to vary in the range 0.31-0.49 μF/cm(2) and 45-65 kΩ cm(2), respectively, corresponding to partially solvent containing BLMs with an average life time of 60-80 min. The characterized hsBLM formation and devised equivalent circuit models lead to a schematic model to illustrate lipid molecule distribution in hydrogel-supported apertures. The functionality of stabilized hsBLMs and detection sensitivity of the platform were verified by monitoring the effect of the ion transporter valinomycin.

77 FR 74470 - Notice of Availability for the Draft Environmental Impact Statement for the Gregory Canyon...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-14

... DEPARTMENT OF DEFENSE Department of the Army, Corps of Engineers Notice of Availability for the Draft Environmental Impact Statement for the Gregory Canyon Landfill Project, San Diego County, CA... Draft Environmental Impact Statement (EIS) for the proposed Gregory Canyon Landfill Project in San Diego...

75 FR 77654 - Notice of Intent To Prepare a Land Use Plan Amendment and an Environmental Impact Statement for...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-13

..., maintenance, and decommissioning of wind turbine generators and associated facilities necessary to... the Pattern Energy Group Ocotillo Express Wind Energy Project, Imperial County, CA AGENCY: Bureau of... Pattern Energy Group Ocotillo Express Wind Energy Project Draft EIR/EIS by any of the following methods...

77 FR 59180 - Withdrawal of Intent To Prepare a Draft Environmental Impact Statement/Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-26

... of Ballona Creek including coastal wetlands. Santa Monica Bay Restoration Commission (SMBRC) is the... Restoration Feasibility Study, Los Angeles County, CA AGENCY: Department of the Army, U.S. Army Corps of.../Environmental Impact Report (EIS/EIR) for the Ballona Creek Ecosystem Restoration Feasibility Study in the...

75 FR 36091 - Notice of Availability of the Draft Environmental Impact Statement for Improvements to the...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-24

... for Improvements to the Calexico West Port of Entry, Calexico, CA AGENCY: Public Buildings Service... Impact Statement (EIS) for Improvements to the Calexico West Port of Entry, Calexico, California, for... and soils) or topic area (e.g., traffic, environmental justice). After the public comment period...

75 FR 13301 - Los Vaqueros Reservoir Expansion, Contra Costa and Alameda Counties, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

... DEPARTMENT OF THE INTERIOR Bureau of Reclamation Los Vaqueros Reservoir Expansion, Contra Costa... Reservoir Expansion Final EIS/EIR. Los Vaqueros Expansion is a proposed action in the August 2000 CALFED Bay... Vaqueros Reservoir from its existing capacity of 100 thousand acre-feet (TAF). A 175 TAF expansion option...

High sensitivity and label-free detection of Enterovirus 71 by nanogold modified electrochemical impedance spectroscopy

NASA Astrophysics Data System (ADS)

Wang, Fang-Yu; Li, Hsing-Yuan; Tseng, Shing-Hua; Cheng, Tsai-Mu; Chu, Hsueh-Liang; Yang, Jyh-Yuan; Chang, Chia-Ching

2013-03-01

Enterovirus 71 (EV71), which is the most fulminant and invasive species of enterovirus, can cause children neurologic complications and death within 2-3 days after fever and rash developed. Besides, EV71 has high sequence similarity with Coxsackie A 16 (CA16) that makes differential diagnosis difficult in clinic and laboratory. Since conventional viral diagnostic method cannot diagnose EV71 quickly and EV71 can transmit at low viral titer, the patients might delay in treatment. A quick, high sensitive, and high specific test for EV71 detection is pivotal. Electrochemical impedance spectroscopy (EIS) has been applied for detecting bio-molecules as biosensors recently. In this study, we try to build a detection platform for EV71 detection by nanogold modified EIS probe. The result shows that our probe can detect 3.6 VP1/50 μl (one EV71 particle has 60 VP1) in 3 minutes. The test can also distinguish EV71 from CA16 and lysozyme. Diagnosis of enterovirus 71 by electrochemical impedance spectroscopy has the potential to apply in clinic.

Operation of the PAVE PAWS Radar System at Beale Air Force Base, California. Part 1. Basic EIS & Appendices.

DTIC Science & Technology

1980-07-01

Middlefield Road Mail Stop 92 Menlo Park, CA 94025 U.S. Geological Survey 2800 Cottage Way Sacramento, CA 95825 State and Local Agencies Air Resources Board...RELATIONSHIP BETWEEN LOCAL SHORT-TERM USE OF MAN’S ENVIRONMT AND THE mAINUKANCE AND ENHANCEMNT OF LONG-TERM PRODUCTIVITY . . . . . . . . . . 6-1 7...Distances from Local Areas of Interest to Mt. lebo, Oregon AFS . . . .. ... 1-41 1-15 lousing Distribution of Personnel Affected by PAVS PAWS Mt.lRebo AlS

Protective effect of dexpanthenol on bleomycin-induced pulmonary fibrosis in rats.

PubMed

Ermis, Hilal; Parlakpinar, Hakan; Gulbas, Gazi; Vardi, Nigar; Polat, Alaadin; Cetin, Asli; Kilic, Talat; Aytemur, Zeynep Ayfer

2013-12-01

Despite extensive studies, there is no effective treatment currently available other than pirfenidone for idiopathic pulmonary fibrosis. A protective effect of pantothenic acid and its derivatives on cell damage produced by oxygen radicals has been reported, but it has not been tested in bleomycin (BLM)--induced pulmonary fibrosis in rats. Therefore, we aimed to investigate the preventive effect of dexpanthenol (Dxp) on pulmonary fibrosis. Thirty-two rats were assigned to four groups as follows: (1) control group, (2) dexpanthenol (Dxp) group; 500 mg/kg Dxp continued intraperitoneally for 14 days, (3) bleomycin (BLM) group; a single intratracheal injection of BLM (2.5 mg/kg body weight in 0.25-ml phosphate buffered saline), and (4) BLM + Dxp-treated group; 500 mg/kg Dxp was administered 1 h before the intratracheal BLM injection and continued for 14 days i.p. The histopathological grades of lung inflammation and collagen deposition, tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and myeloperoxidase (MPO) were measured. BLM provoked inflammation and collagen deposition (p < 0.0001), with a marked increase in myeloperoxidase (MPO) activity resembling increased inflammatory activity (p < 0.0001), which was prevented by Dxp (p < 0.0001, p = 0.02). BLM reduced tissue activities of SOD, GPx, and CAT compared to controls (p = 0.01, 0.03, 0.009). MDA was increased with BLM (p = 0.003). SOD (p = 0.001) and MDA (p = 0.016) levels were improved in group 4. The CAT levels in the BLM + Dxp group were close to those in the control group (p > 0.05). We showed that Dxp significantly prevents BLM-induced lung fibrosis in rats. Further studies are required to evaluate the role of Dxp in the treatment of lung fibrosis.

Protective Effect of Onion Extract on Bleomycin-Induced Cytotoxicity and Genotoxicity in Human Lymphocytes

PubMed Central

Cho, Yoon Hee; Lee, Joong Won; Woo, Hae Dong; Lee, Sunyeong; Kim, Yang Jee; Lee, Younghyun; Shin, Sangah; Joung, Hyojee; Chung, Hai Won

2016-01-01

Following one of the world’s largest nuclear accidents, occured at Fukushima, Japan in 2011, a significant scientific effort has focused on minimizing the potential adverse health effects due to radiation exposure. The use of natural dietary antioxidants to reduce the risk of radiation-induced oxidative DNA damage is a simple strategy for minimizing radiation-related cancer rates and improving overall health. The onion is among the richest sources of dietary flavonoids and is an important food for increasing their overall intake. Therefore, we examined the effect of an onion extract on cyto- and geno-toxicity in human lymphocytes treated with bleomycin (BLM), a radiomimetic agent. In addition, we measured the frequency of micronuclei (MN) and DNA damage following treatment with BLM using a cytokinesis-blocked micronucleus assay and a single cell gel electrophoresis assay. We observed a significant increase in cell viability in lymphocytes treated with onion extract then exposed to BLM compared to cells treated with BLM alone. The frequency of BLM induced MN and DNA damage increased in a dose-dependent manner; however, when lymphocytes were pretreated with onion extract (10 and 20 μL/mL), the frequency of BLM-induced MN was decreased at all doses of BLM and DNA damage was decreased at 3 μg/mL of BLM. These results suggest that onion extract may have protective effects against BLM-induced cyto- and genotoxicity in human lymphocytes. PMID:26907305

Protective Effect of Onion Extract on Bleomycin-Induced Cytotoxicity and Genotoxicity in Human Lymphocytes.

PubMed

Cho, Yoon Hee; Lee, Joong Won; Woo, Hae Dong; Lee, Sunyeong; Kim, Yang Jee; Lee, Younghyun; Shin, Sangah; Joung, Hyojee; Chung, Hai Won

2016-02-19

Following one of the world's largest nuclear accidents, occured at Fukushima, Japan in 2011, a significant scientific effort has focused on minimizing the potential adverse health effects due to radiation exposure. The use of natural dietary antioxidants to reduce the risk of radiation-induced oxidative DNA damage is a simple strategy for minimizing radiation-related cancer rates and improving overall health. The onion is among the richest sources of dietary flavonoids and is an important food for increasing their overall intake. Therefore, we examined the effect of an onion extract on cyto- and geno-toxicity in human lymphocytes treated with bleomycin (BLM), a radiomimetic agent. In addition, we measured the frequency of micronuclei (MN) and DNA damage following treatment with BLM using a cytokinesis-blocked micronucleus assay and a single cell gel electrophoresis assay. We observed a significant increase in cell viability in lymphocytes treated with onion extract then exposed to BLM compared to cells treated with BLM alone. The frequency of BLM induced MN and DNA damage increased in a dose-dependent manner; however, when lymphocytes were pretreated with onion extract (10 and 20 μL/mL), the frequency of BLM-induced MN was decreased at all doses of BLM and DNA damage was decreased at 3 μg/mL of BLM. These results suggest that onion extract may have protective effects against BLM-induced cyto- and genotoxicity in human lymphocytes.

43 CFR 2886.15 - How is grant or TUP administration affected if the BLM land my grant or TUP encumbers is...

Code of Federal Regulations, 2012 CFR

2012-10-01

... affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of... administration affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of Federal ownership? (a) If there is a proposal to transfer the BLM land your grant or TUP encumbers...

43 CFR 2886.15 - How is grant or TUP administration affected if the BLM land my grant or TUP encumbers is...

Code of Federal Regulations, 2011 CFR

2011-10-01

... affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of... administration affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of Federal ownership? (a) If there is a proposal to transfer the BLM land your grant or TUP encumbers...

43 CFR 2886.15 - How is grant or TUP administration affected if the BLM land my grant or TUP encumbers is...

Code of Federal Regulations, 2013 CFR

2013-10-01

... affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of... administration affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of Federal ownership? (a) If there is a proposal to transfer the BLM land your grant or TUP encumbers...

RECQ-like helicases Sgs1 and BLM regulate R-loop–associated genome instability

PubMed Central

Chang, Emily Yun-Chia; Novoa, Carolina A.; Aristizabal, Maria J.; Coulombe, Yan; Segovia, Romulo; Shen, Yaoqing; Keong, Christelle; Tam, Annie S.; Jones, Steven J.M.; Masson, Jean-Yves; Kobor, Michael S.

2017-01-01

Sgs1, the orthologue of human Bloom’s syndrome helicase BLM, is a yeast DNA helicase functioning in DNA replication and repair. We show that SGS1 loss increases R-loop accumulation and sensitizes cells to transcription–replication collisions. Yeast lacking SGS1 accumulate R-loops and γ-H2A at sites of Sgs1 binding, replication pausing regions, and long genes. The mutation signature of sgs1Δ reveals copy number changes flanked by repetitive regions with high R-loop–forming potential. Analysis of BLM in Bloom’s syndrome fibroblasts or by depletion of BLM from human cancer cells confirms a role for Sgs1/BLM in suppressing R-loop–associated genome instability across species. In support of a potential direct effect, BLM is found physically proximal to DNA:RNA hybrids in human cells, and can efficiently unwind R-loops in vitro. Together, our data describe a conserved role for Sgs1/BLM in R-loop suppression and support an increasingly broad view of DNA repair and replication fork stabilizing proteins as modulators of R-loop–mediated genome instability. PMID:29042409

Dynamics of the DNA repair proteins WRN and BLM in the nucleoplasm and nucleoli.

PubMed

Bendtsen, Kristian Moss; Jensen, Martin Borch; May, Alfred; Rasmussen, Lene Juel; Trusina, Ala; Bohr, Vilhelm A; Jensen, Mogens H

2014-11-01

We have investigated the mobility of two EGFP-tagged DNA repair proteins, WRN and BLM. In particular, we focused on the dynamics in two locations, the nucleoli and the nucleoplasm. We found that both WRN and BLM use a "DNA-scanning" mechanism, with rapid binding-unbinding to DNA resulting in effective diffusion. In the nucleoplasm WRN and BLM have effective diffusion coefficients of 1.62 and 1.34 μm(2)/s, respectively. Likewise, the dynamics in the nucleoli are also best described by effective diffusion, but with diffusion coefficients a factor of ten lower than in the nucleoplasm. From this large reduction in diffusion coefficient we were able to classify WRN and BLM as DNA damage scanners. In addition to WRN and BLM we also classified other DNA damage proteins and found they all fall into one of two categories. Either they are scanners, similar to WRN and BLM, with very low diffusion coefficients, suggesting a scanning mechanism, or they are almost freely diffusing, suggesting that they interact with DNA only after initiation of a DNA damage response.

Combined cytogenotoxic effects of bee venom and bleomycin on rat lymphocytes: an in vitro study.

PubMed

Abd-Elhakim, Yasmina M; Khalil, Samah R; Awad, Ashraf; Al-Ayadhi, Laila Y

2014-01-01

This study was carried out to determine the cytotoxic and genotoxic effects of bee venom (BV) and/or the chemotherapeutic agent bleomycin (BLM) on healthy isolated rat lymphocytes utilizing morphometric and molecular techniques. Using the Ficoll-Histopaque density gradient centrifugation technique, lymphocytes were isolated, divided into groups, and subjected to BV and/or BLM at incubation medium concentrations of 10 or 20 μg/mL respectively for 24 and 72 hrs. An MTT assay and fluorescent microscopy examinations were used to assess the cytotoxic effects. To determine the predominant type of BV and/or BLM-induced cell death, LDH release assay was employed beside quantitative expression analyses of the apoptosis-related genes (Caspase-3 and Bcl-2). The genotoxic effects of the tested compounds were evaluated via DNA fragmentation assay. The results of these assays demonstrated that BV potentiates BLM-induced cytotoxicity through increased LDH release and diminished cell viability. Nevertheless, BV significantly inhibited the BLM-induced DNA damage. The results verify that BV significantly attenuates the genotoxic effects of BLM on noncancerous isolated rat lymphocytes but does not diminish BLM cytotoxicity.

RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability.

PubMed

Chang, Emily Yun-Chia; Novoa, Carolina A; Aristizabal, Maria J; Coulombe, Yan; Segovia, Romulo; Chaturvedi, Richa; Shen, Yaoqing; Keong, Christelle; Tam, Annie S; Jones, Steven J M; Masson, Jean-Yves; Kobor, Michael S; Stirling, Peter C

2017-12-04

Sgs1, the orthologue of human Bloom's syndrome helicase BLM, is a yeast DNA helicase functioning in DNA replication and repair. We show that SGS1 loss increases R-loop accumulation and sensitizes cells to transcription-replication collisions. Yeast lacking SGS1 accumulate R-loops and γ-H2A at sites of Sgs1 binding, replication pausing regions, and long genes. The mutation signature of sgs1 Δ reveals copy number changes flanked by repetitive regions with high R-loop-forming potential. Analysis of BLM in Bloom's syndrome fibroblasts or by depletion of BLM from human cancer cells confirms a role for Sgs1/BLM in suppressing R-loop-associated genome instability across species. In support of a potential direct effect, BLM is found physically proximal to DNA:RNA hybrids in human cells, and can efficiently unwind R-loops in vitro. Together, our data describe a conserved role for Sgs1/BLM in R-loop suppression and support an increasingly broad view of DNA repair and replication fork stabilizing proteins as modulators of R-loop-mediated genome instability. © 2017 Chang et al.

An ultra-sensitive impedimetric immunosensor for detection of the serum oncomarker CA-125 in ovarian cancer patients

NASA Astrophysics Data System (ADS)

Johari-Ahar, M.; Rashidi, M. R.; Barar, J.; Aghaie, M.; Mohammadnejad, D.; Ramazani, A.; Karami, P.; Coukos, G.; Omidi, Y.

2015-02-01

Effective treatment of ovarian cancer depends upon the early detection of the malignancy. Here, we report on the development of a new nanostructured immunosensor for early detection of cancer antigen 125 (CA-125). A gold electrode was modified with mercaptopropionic acid (MPA), and then consecutively conjugated with silica coated gold nanoparticles (AuNP@SiO2), CdSe quantum dots (QDs) and anti-CA-125 monoclonal antibody (mAb). The engineered MPA|AuNP@SiO2|QD|mAb immunosensor was characterised using transmission electron microscopy (TEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Successive conjugation of AuNP@SiO2, CdSe QD and anti-CA-125 mAb onto the gold electrode resulted in sensitive detection of CA-125 with a limit of detection (LOD) of 0.0016 U mL-1 and a linear detection range (LDR) of 0-0.1 U mL-1. Based on the high sensitivity and specificity of the immunosensor, we propose this highly stable and reproducible biosensor for the early detection of CA-125.Effective treatment of ovarian cancer depends upon the early detection of the malignancy. Here, we report on the development of a new nanostructured immunosensor for early detection of cancer antigen 125 (CA-125). A gold electrode was modified with mercaptopropionic acid (MPA), and then consecutively conjugated with silica coated gold nanoparticles (AuNP@SiO2), CdSe quantum dots (QDs) and anti-CA-125 monoclonal antibody (mAb). The engineered MPA|AuNP@SiO2|QD|mAb immunosensor was characterised using transmission electron microscopy (TEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Successive conjugation of AuNP@SiO2, CdSe QD and anti-CA-125 mAb onto the gold electrode resulted in sensitive detection of CA-125 with a limit of detection (LOD) of 0.0016 U mL-1 and a linear detection range (LDR) of 0-0.1 U mL-1. Based on the high sensitivity and specificity of the immunosensor, we propose this highly stable and reproducible biosensor for the early detection of CA-125. Electronic supplementary information (ESI) available: Additional materials including Figures and discussion as described in the text. See DOI: 10.1039/c4nr06687a

75 FR 19422 - Notice of Closure of Airport Mesa/Carizzo Creek Shooting Area in Eastern San Diego County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-14

...The Bureau of Land Management (BLM) has closed approximately 210 acres of public land described as the Airport Mesa/Carrizo Creek shooting area located in eastern San Diego County, California. The closure order prohibits recreational shooting and target practice. The use of firearms will continue to be allowed for hunting consistent with California Department of Fish and Game regulations and seasons. This closure order is necessary in order to protect U.S. Border Patrol agents as they perform their duties along the top of Airport Mesa.

43 CFR 3715.7-1 - What types of enforcement action can BLM take if I do not meet the requirements of this subpart?

Code of Federal Regulations, 2011 CFR

2011-10-01

... Laws § 3715.7-1 What types of enforcement action can BLM take if I do not meet the requirements of this... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false What types of enforcement action can BLM... necessary to protect health, safety or the environment. (2) BLM will presume that health, safety or the...

43 CFR 3715.7-1 - What types of enforcement action can BLM take if I do not meet the requirements of this subpart?

Code of Federal Regulations, 2012 CFR

2012-10-01

... Laws § 3715.7-1 What types of enforcement action can BLM take if I do not meet the requirements of this... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false What types of enforcement action can BLM... necessary to protect health, safety or the environment. (2) BLM will presume that health, safety or the...

43 CFR 3715.7-1 - What types of enforcement action can BLM take if I do not meet the requirements of this subpart?

Code of Federal Regulations, 2014 CFR

2014-10-01

... Laws § 3715.7-1 What types of enforcement action can BLM take if I do not meet the requirements of this... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false What types of enforcement action can BLM... necessary to protect health, safety or the environment. (2) BLM will presume that health, safety or the...

43 CFR 3715.7-1 - What types of enforcement action can BLM take if I do not meet the requirements of this subpart?

Code of Federal Regulations, 2013 CFR

2013-10-01

... Laws § 3715.7-1 What types of enforcement action can BLM take if I do not meet the requirements of this... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false What types of enforcement action can BLM... necessary to protect health, safety or the environment. (2) BLM will presume that health, safety or the...

Electrochemical characterization of bilayer lipid membrane-semiconductor junctions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, Xiao Kang; Baral, S.; Fendler, J.H.

Three different systems of glyceryl monooleate (GMO), bilayer lipid membrane (BLM) supported semiconductor particles have been prepared and characterized. A single composition of particulate semiconductor deposited only on one side of the BLM constituted system A, two different compositions of particulate semiconductors sequentially deposited on the same side of the BLM represented system B, and two different compositions of particulate semiconductors deposited on the opposite sides of the BLM made up system C.

Variation of the NMVOC speciation in the solvent sector and the sensitivity of modelled tropospheric ozone

NASA Astrophysics Data System (ADS)

von Schneidemesser, E.; Coates, J.; Denier van der Gon, H. A. C.; Visschedijk, A. J. H.; Butler, T. M.

2016-06-01

Non-methane volatile organic compounds (NMVOCs) are detrimental to human health owing to the toxicity of many of the NMVOC species, as well as their role in the formation of secondary air pollutants such as tropospheric ozone (O3) and secondary organic aerosol. The speciation and amount of NMVOCs emitted into the troposphere are represented in emission inventories (EIs) for input to chemical transport models that predict air pollutant levels. Much of the information in EIs pertaining to speciation of NMVOCs is likely outdated, but before taking on the task of providing an up-to-date and highly speciated EI, a better understanding of the sensitivity of models to the change in NMVOC input would be highly beneficial. According to the EIs, the solvent sector is the most important sector for NMVOC emissions. Here, the sensitivity of modelled tropospheric O3 to NMVOC emission inventory speciation was investigated by comparing the maximum potential difference in O3 produced using a variety of reported solvent sector EI speciations in an idealized study using a box model. The sensitivity was tested using three chemical mechanisms that describe O3 production chemistry, typically employed for different types of modelling scales - point (MCM v3.2), regional (RADM2), and global (MOZART-4). In the box model simulations, a maximum difference of 15 ppbv (ca. 22% of the mean O3 mixing ratio of 69 ppbv) between the different EI speciations of the solvent sector was calculated. In comparison, for the same EI speciation, but comparing the three different mechanisms, a maximum difference of 6.7 ppbv was observed. Relationships were found between the relative contribution of NMVOC compound classes (alkanes and oxygenated species) in the speciations to the amount of Ox produced in the box model. These results indicate that modelled tropospheric O3 is sensitive to the speciation of NMVOCs as specified by emission inventories, suggesting that detailed updates to the EI speciation information would be warranted. Furthermore, modelled tropospheric O3 was also sensitive to the choice of chemical mechanism and further evaluation of both of these sensitivities in more realistic chemical-transport models is needed.

Supercritical-Carbon Dioxide Fluid Extract from Chrysanthemum indicum Enhances Anti-Tumor Effect and Reduces Toxicity of Bleomycin in Tumor-Bearing Mice

PubMed Central

Yang, Hong-Mei; Sun, Chao-Yue; Liang, Jia-Li; Xu, Lie-Qiang; Zhang, Zhen-Biao; Luo, Dan-Dan; Chen, Han-Bin; Huang, Yong-Zhong; Wang, Qi; Lee, David Yue-Wei; Yuan, Jie; Li, Yu-Cui

2017-01-01

Bleomycin (BLM), a family of anti-tumor drugs, was reported to exhibit severe side effects limiting its usage in clinical treatment. Therefore, finding adjuvants that enhance the anti-tumor effect and reduce the detrimental effect of BLM is a prerequisite. Chrysanthemum indicum, an edible flower, possesses abundant bioactivities; the supercritical-carbon dioxide fluid extract from flowers and buds of C. indicum (CISCFE) have strong anti-inflammatory, anti-oxidant, and lung protective effects. However, the role of CISCFE combined with BLM treatment on tumor-bearing mice remains unclear. The present study aimed to investigate the potential synergistic effect and the underlying mechanism of CISCFE combined with BLM in the treatment of hepatoma 22 (H22) tumor-bearing mice. The results suggested that the oral administration of CISCFE combined with BLM could markedly prolong the life span, attenuate the BLM-induced pulmonary fibrosis, suppress the production of pro-inflammatory cytokines (interleukin-6), tumor necrosis factor-α, activities of myeloperoxidase, and malondiadehyde. Moreover, CISCFE combined with BLM promoted the ascites cell apoptosis, the activities of caspases 3 and 8, and up-regulated the protein expression of p53 and down-regulated the transforming growth factor-β1 by activating the gene expression of miR-29b. Taken together, these results indicated that CISCFE could enhance the anti-cancer activity of BLM and reduce the BLM-induced pulmonary injury in H22 tumor-bearing mice, rendering it as a potential adjuvant drug with chemotherapy after further investigation in the future. PMID:28245556

Optimization of a murine and human tissue model to recapitulate dermal and pulmonary features of systemic sclerosis

PubMed Central

Watanabe, Tomoya; Mlakar, Logan; Heywood, Jonathan; Malaab, Maya; Hoffman, Stanley

2017-01-01

The murine bleomycin (BLM)-induced fibrosis model is the most widely used in systemic sclerosis (SSc) studies. It has been reported that systemic delivery of BLM via continuous diffusion from subcutaneously implanted osmotic minipumps can cause fibrosis of the skin, lungs, and other internal organs. However, the mouse strain, dosage of BLM, administration period, and additional important features differ from one report to the next. In this study, by employing the pump model in C57BL/6J mice, we show a dose-dependent increase in lung fibrosis by day 28 and a transient increase in dermal thickness. Dermal thickness and the level of collagen in skin treated with high-dose BLM was significantly higher than in skin treated with low dose BLM or vehicle. A reduction in the thickness of the adipose layer was noted in both high and low dose groups at earlier time points suggesting that the loss of the fat layer precedes the onset of fibrosis. High-dose BLM also induced dermal fibrosis and increased expression of fibrosis-associated genes ex vivo in human skin, thus confirming and extending the in vivo findings, and demonstrating that a human organ culture model can be used to assess the effect of BLM on skin. In summary, our findings suggest that the BLM pump model is an attractive model to analyze the underlying mechanisms of fibrosis and test the efficacy of potential therapies. However, the choice of mouse strain, duration of BLM administration and dose must be carefully considered when using this model. PMID:28651005

Optimization of a murine and human tissue model to recapitulate dermal and pulmonary features of systemic sclerosis.

PubMed

Watanabe, Tomoya; Nishimoto, Tetsuya; Mlakar, Logan; Heywood, Jonathan; Malaab, Maya; Hoffman, Stanley; Feghali-Bostwick, Carol

2017-01-01

The murine bleomycin (BLM)-induced fibrosis model is the most widely used in systemic sclerosis (SSc) studies. It has been reported that systemic delivery of BLM via continuous diffusion from subcutaneously implanted osmotic minipumps can cause fibrosis of the skin, lungs, and other internal organs. However, the mouse strain, dosage of BLM, administration period, and additional important features differ from one report to the next. In this study, by employing the pump model in C57BL/6J mice, we show a dose-dependent increase in lung fibrosis by day 28 and a transient increase in dermal thickness. Dermal thickness and the level of collagen in skin treated with high-dose BLM was significantly higher than in skin treated with low dose BLM or vehicle. A reduction in the thickness of the adipose layer was noted in both high and low dose groups at earlier time points suggesting that the loss of the fat layer precedes the onset of fibrosis. High-dose BLM also induced dermal fibrosis and increased expression of fibrosis-associated genes ex vivo in human skin, thus confirming and extending the in vivo findings, and demonstrating that a human organ culture model can be used to assess the effect of BLM on skin. In summary, our findings suggest that the BLM pump model is an attractive model to analyze the underlying mechanisms of fibrosis and test the efficacy of potential therapies. However, the choice of mouse strain, duration of BLM administration and dose must be carefully considered when using this model.

Supercritical-Carbon Dioxide Fluid Extract from Chrysanthemum indicum Enhances Anti-Tumor Effect and Reduces Toxicity of Bleomycin in Tumor-Bearing Mice.

PubMed

Yang, Hong-Mei; Sun, Chao-Yue; Liang, Jia-Li; Xu, Lie-Qiang; Zhang, Zhen-Biao; Luo, Dan-Dan; Chen, Han-Bin; Huang, Yong-Zhong; Wang, Qi; Lee, David Yue-Wei; Yuan, Jie; Li, Yu-Cui

2017-02-24

Bleomycin (BLM), a family of anti-tumor drugs, was reported to exhibit severe side effects limiting its usage in clinical treatment. Therefore, finding adjuvants that enhance the anti-tumor effect and reduce the detrimental effect of BLM is a prerequisite. Chrysanthemum indicum , an edible flower, possesses abundant bioactivities; the supercritical-carbon dioxide fluid extract from flowers and buds of C. indicum (CI SCFE ) have strong anti-inflammatory, anti-oxidant, and lung protective effects. However, the role of CI SCFE combined with BLM treatment on tumor-bearing mice remains unclear. The present study aimed to investigate the potential synergistic effect and the underlying mechanism of CI SCFE combined with BLM in the treatment of hepatoma 22 (H22) tumor-bearing mice. The results suggested that the oral administration of CI SCFE combined with BLM could markedly prolong the life span, attenuate the BLM-induced pulmonary fibrosis, suppress the production of pro-inflammatory cytokines (interleukin-6), tumor necrosis factor-α, activities of myeloperoxidase, and malondiadehyde. Moreover, CI SCFE combined with BLM promoted the ascites cell apoptosis, the activities of caspases 3 and 8, and up-regulated the protein expression of p53 and down-regulated the transforming growth factor-β1 by activating the gene expression of miR-29b. Taken together, these results indicated that CI SCFE could enhance the anti-cancer activity of BLM and reduce the BLM-induced pulmonary injury in H22 tumor-bearing mice, rendering it as a potential adjuvant drug with chemotherapy after further investigation in the future.

43 CFR 3836.25 - What if BLM denies my petition for deferment of assessment work?

Code of Federal Regulations, 2011 CFR

2011-10-01

...) ANNUAL ASSESSMENT WORK REQUIREMENTS FOR MINING CLAIMS Deferring Assessment Work § 3836.25 What if BLM... BLM decision denying the petition in which to pay the maintenance fee to maintain your claim. ...

43 CFR 3836.25 - What if BLM denies my petition for deferment of assessment work?

Code of Federal Regulations, 2012 CFR

2012-10-01

...) ANNUAL ASSESSMENT WORK REQUIREMENTS FOR MINING CLAIMS Deferring Assessment Work § 3836.25 What if BLM... BLM decision denying the petition in which to pay the maintenance fee to maintain your claim. ...

43 CFR 3836.25 - What if BLM denies my petition for deferment of assessment work?

Code of Federal Regulations, 2014 CFR

2014-10-01

...) ANNUAL ASSESSMENT WORK REQUIREMENTS FOR MINING CLAIMS Deferring Assessment Work § 3836.25 What if BLM... BLM decision denying the petition in which to pay the maintenance fee to maintain your claim. ...

43 CFR 3836.25 - What if BLM denies my petition for deferment of assessment work?

Code of Federal Regulations, 2013 CFR

2013-10-01

...) ANNUAL ASSESSMENT WORK REQUIREMENTS FOR MINING CLAIMS Deferring Assessment Work § 3836.25 What if BLM... BLM decision denying the petition in which to pay the maintenance fee to maintain your claim. ...

43 CFR 3261.18 - Do I need to file a bond with BLM before I build a well pad or drill a well?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Do I need to file a bond with BLM before I...) GEOTHERMAL RESOURCE LEASING Drilling Operations: Getting a Permit § 3261.18 Do I need to file a bond with BLM before I build a well pad or drill a well? Before starting any operation, you must: (a) File with BLM...

Final Base Realignment and Closure (BRAC) Environmental Assessment for Realignment of Nellis Air Force Base

DTIC Science & Technology

2007-03-01

Traffic Control Assigned Airspace ATG Adversary Tactics Group AWACS Airborne Warning and Control System BAQ Bureau of Air Quality BLM Bureau of Land...Department of Interior Actions BLM The BLM manages millions of acres of public lands in southern Nevada which include portions of NTTR and...within NTTR and would not affect BLM lands adjacent to the base. Therefore, there are no cumulative impacts. USFWS Aircraft operate within the

BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription

PubMed Central

Grierson, Patrick M.; Lillard, Kate; Behbehani, Gregory K.; Combs, Kelly A.; Bhattacharyya, Saumitri; Acharya, Samir; Groden, Joanna

2012-01-01

Bloom's syndrome (BS) is an autosomal recessive disorder that is invariably characterized by severe growth retardation and cancer predisposition. The Bloom's syndrome helicase (BLM), mutations of which lead to BS, localizes to promyelocytic leukemia protein bodies and to the nucleolus of the cell, the site of RNA polymerase I-mediated ribosomal RNA (rRNA) transcription. rRNA transcription is fundamental for ribosome biogenesis and therefore protein synthesis, cellular growth and proliferation; its inhibition limits cellular growth and proliferation as well as bodily growth. We report that nucleolar BLM facilitates RNA polymerase I-mediated rRNA transcription. Immunofluorescence studies demonstrate the dependance of BLM nucleolar localization upon ongoing RNA polymerase I-mediated rRNA transcription. In vivo protein co-immunoprecipitation demonstrates that BLM interacts with RPA194, a subunit of RNA polymerase I. 3H-uridine pulse-chase assays demonstrate that BLM expression is required for efficient rRNA transcription. In vitro helicase assays demonstrate that BLM unwinds GC-rich rDNA-like substrates that form in the nucleolus and normally inhibit progression of the RNA polymerase I transcription complex. These studies suggest that nucleolar BLM modulates rDNA structures in association with RNA polymerase I to facilitate RNA polymerase I-mediated rRNA transcription. Given the intricate relationship between rDNA metabolism and growth, our data may help in understanding the etiology of proportional dwarfism in BS. PMID:22106380

BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription.

PubMed

Grierson, Patrick M; Lillard, Kate; Behbehani, Gregory K; Combs, Kelly A; Bhattacharyya, Saumitri; Acharya, Samir; Groden, Joanna

2012-03-01

Bloom's syndrome (BS) is an autosomal recessive disorder that is invariably characterized by severe growth retardation and cancer predisposition. The Bloom's syndrome helicase (BLM), mutations of which lead to BS, localizes to promyelocytic leukemia protein bodies and to the nucleolus of the cell, the site of RNA polymerase I-mediated ribosomal RNA (rRNA) transcription. rRNA transcription is fundamental for ribosome biogenesis and therefore protein synthesis, cellular growth and proliferation; its inhibition limits cellular growth and proliferation as well as bodily growth. We report that nucleolar BLM facilitates RNA polymerase I-mediated rRNA transcription. Immunofluorescence studies demonstrate the dependance of BLM nucleolar localization upon ongoing RNA polymerase I-mediated rRNA transcription. In vivo protein co-immunoprecipitation demonstrates that BLM interacts with RPA194, a subunit of RNA polymerase I. (3)H-uridine pulse-chase assays demonstrate that BLM expression is required for efficient rRNA transcription. In vitro helicase assays demonstrate that BLM unwinds GC-rich rDNA-like substrates that form in the nucleolus and normally inhibit progression of the RNA polymerase I transcription complex. These studies suggest that nucleolar BLM modulates rDNA structures in association with RNA polymerase I to facilitate RNA polymerase I-mediated rRNA transcription. Given the intricate relationship between rDNA metabolism and growth, our data may help in understanding the etiology of proportional dwarfism in BS.

75 FR 9399 - Notice of Availability of a Final General Conformity Determination and Record of Decision for the...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-02

... DEPARTMENT OF DEFENSE Department of the Army Corps of Engineers Notice of Availability of a Final... of Long Beach, Los Angeles County, CA AGENCY: Department of the Army--U.S. Army Corps of Engineers... and published a joint Final Environmental Impact Statement/Environmental Impact Report (EIS/EIR) for...

76 FR 29261 - Notice of Intent To Prepare an Environmental Impact Statement for the Proposed Campo Wind Energy...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-20

... Impact Statement for the Proposed Campo Wind Energy Project, San Diego County, CA AGENCY: Bureau of... (EIS) for the proposed Campo Shu'luuk Wind Project, located on the Campo Indian Reservation in...'luuk Wind Project within the Campo Reservation is subject to BIA approval of a lease and sublease...

75 FR 6057 - Notice of Availability of the Draft Environmental Impact Statement for the Proposed Chevron...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-05

... right-of-way (ROW) authorization to construct and operate a 45-megawatt solar photovoltaic project and... Energy Solutions Lucerne Valley Solar Project, San Bernardino County, CA, and the Draft California Desert... Statement (EIS) for the proposed Chevron Energy Solutions Lucerne Valley Solar Project and by this notice is...

76 FR 54452 - Intent to Prepare a Draft Environmental Impact Statement for the Proposed Cordova Hills Project...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-01

... DEPARTMENT OF DEFENSE Department of the Army, Corps of Engineers Intent to Prepare a Draft Environmental Impact Statement for the Proposed Cordova Hills Project in Sacramento County, CA, Corps Permit.... Potentially significant issues to be analyzed in depth in the EIS include loss of waters of the United States...

Bleomycin-induced epithelial–mesenchymal transition in sclerotic skin of mice: Possible role of oxidative stress in the pathogenesis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Cheng-Fan, E-mail: zhouchengfan@sohu.com; Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui 230032; Zhou, Deng-Chuan

Epithelial–mesenchymal transition (EMT) derived myofibroblasts are partly responsible for the increased collagen synthesis and deposition that occur in tissue fibrosis; however EMT occurrence in skin fibrosis and its mechanism remain unknown. The aim of this study was to investigate whether epithelial cells undergo EMT and determine the role of oxidative stress in this process. BALB/c mice were subcutaneously injected with bleomycin (BLM) or phosphate buffer saline (PBS) into the shaved back daily for 2, 3, and 4 weeks. Skin collagen deposition was evaluated by histopathology and Western blotting. EMT characteristics in the skin were determined by histopathology and immunofluorescent stainingmore » for E-cadherin and vimentin, which were further evaluated by Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR). To investigate the role of oxidative stress in EMT, the antioxidant N-acetylcysteine (NAC) was intraperitoneally (100 mg/kg body weight/day) injected daily for 3 weeks. The epithelial suprabasal cells were detached from the basement membrane zone (BMZ) in the sclerotic skin treated with BLM. Immunofluorescent staining indicated vimentin-positive epithelial cells frequently occurring in the thickened epidermis of BLM-treated mice. Western blotting and RT-PCR showed that the expression of E-cadherin was significantly decreased but that of vimentin significantly increased in the skin treated with BLM. NAC attenuated BLM induced oxidative damage, changes in E-cadherin and vimentin expressions and collagen deposition in the sclerotic skin of mice. This study provides the first evidence that BLM induces the EMT of the epithelial cells superficial to the basement membrane zone in the skin fibrosis. Oxidative stress may contribute, at least in part, to BLM induced EMT and skin fibrosis in mice. - Highlights: • We provided the first evidence that EMT occurred in BLM-induced skin fibrosis. • Epithelial cells superficial to the BMZ underwent EMT in BLM-induced skin fibrosis. • NAC attenuated EMT induction in the process of BLM-induced skin fibrosis. • NAC didn't significantly inhibit TGF-β expression in BLM-induced skin fibrosis.« less

Acetylcholinesterase inhibition in cognition-relevant brain areas of mice treated with a nootropic Amazonian herbal (Marapuama).

PubMed

Figueiró, M; Ilha, J; Pochmann, D; Porciúncula, L O; Xavier, L L; Achaval, M; Nunes, D S; Elisabetsky, E

2010-10-01

The goal of acetylcholinesterase inhibitors (AChEIs) used to treat Alzheimer's patients is an improvement in cholinergic transmission. While currently available AChEIs have limited success, a huge impediment to the development of newer ones is access to the relevant brain areas. Promnesic, anti-amnesic and AChEI properties were identified in a standardized ethanol extract from Ptychopetalum olacoides (POEE), a medicinal plant favored by the elderly in Amazon communities. The purpose of this study was to provide conclusive evidence that orally given POEE induces AChE inhibition in brain areas relevant to cognition. Histochemistry experiments confirmed that the anticholinesterase compound(s) present in POEE are orally bioavailable, inducing meaningful AChE inhibition in the hippocampus CA1 (∼33%) and CA3 (∼20%), and striatum (∼17%). Ellman's colorimetric analysis revealed that G1 and G4 AChE isoforms activities were markedly inhibited (66 and 72%, respectively) in hippocampus and frontal cortex (50 and 63%, respectively), while G4 appeared to be selectively inhibited (72%) in the striatum. Western blotting showed that POEE did not induce significant changes in the AChE immunocontent suggesting that its synthesis is not extensively modified. This study provides definitive proof of meaningful anticholinesterase activity compatible with the observed promnesic and anti-amnesic effects of POEE in mice, reaffirming the potential of this extract for treating neurodegenerative conditions where a hypofunctioning cholinergic neurotransmission is prominent. Adequate assessment of the safety and efficacy of this extract and/or its isolated active compound(s) are warranted. 2010 Elsevier GmbH. All rights reserved.

Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes: An In Vitro Study

PubMed Central

Abd-Elhakim, Yasmina M.; Khalil, Samah R.; Awad, Ashraf; AL-Ayadhi, Laila Y.

2014-01-01

This study was carried out to determine the cytotoxic and genotoxic effects of bee venom (BV) and/or the chemotherapeutic agent bleomycin (BLM) on healthy isolated rat lymphocytes utilizing morphometric and molecular techniques. Using the Ficoll-Histopaque density gradient centrifugation technique, lymphocytes were isolated, divided into groups, and subjected to BV and/or BLM at incubation medium concentrations of 10 or 20 μg/mL respectively for 24 and 72 hrs. An MTT assay and fluorescent microscopy examinations were used to assess the cytotoxic effects. To determine the predominant type of BV and/or BLM-induced cell death, LDH release assay was employed beside quantitative expression analyses of the apoptosis-related genes (Caspase-3 and Bcl-2). The genotoxic effects of the tested compounds were evaluated via DNA fragmentation assay. The results of these assays demonstrated that BV potentiates BLM-induced cytotoxicity through increased LDH release and diminished cell viability. Nevertheless, BV significantly inhibited the BLM-induced DNA damage. The results verify that BV significantly attenuates the genotoxic effects of BLM on noncancerous isolated rat lymphocytes but does not diminish BLM cytotoxicity. PMID:24822179

Copper bioavailability and toxicity to Mytilus galloprovincialis in Shelter Island Yacht Basin, San Diego, CA.

PubMed

Bosse, Casey; Rosen, Gunther; Colvin, Marienne; Earley, Patrick; Santore, Robert; Rivera-Duarte, Ignacio

2014-08-15

The bioavailability and toxicity of copper (Cu) in Shelter Island Yacht Basin (SIYB), San Diego, CA, USA, was assessed with simultaneous toxicological, chemical, and modeling approaches. Toxicological measurements included laboratory toxicity testing with Mytilus galloprovincialis (Mediterranean mussel) embryos added to both site water (ambient) and site water spiked with multiple Cu concentrations. Chemical assessment of ambient samples included total and dissolved Cu concentrations, and Cu complexation capacity measurements. Modeling was based on chemical speciation and predictions of bioavailability and toxicity using a marine Biotic Ligand Model (BLM). Cumulatively, these methods assessed the natural buffering capacity of Cu in SIYB during singular wet and dry season sampling events. Overall, the three approaches suggested negligible bioavailability, and isolated observed or predicted toxicity, despite an observed gradient of increasing Cu concentration, both horizontally and vertically within the water body, exceeding current water quality criteria for saltwater. Published by Elsevier Ltd.

Bloom DNA Helicase Facilitates Homologous Recombination between Diverged Homologous Sequences*

PubMed Central

Kikuchi, Koji; Abdel-Aziz, H. Ismail; Taniguchi, Yoshihito; Yamazoe, Mitsuyoshi; Takeda, Shunichi; Hirota, Kouji

2009-01-01

Bloom syndrome caused by inactivation of the Bloom DNA helicase (Blm) is characterized by increases in the level of sister chromatid exchange, homologous recombination (HR) associated with cross-over. It is therefore believed that Blm works as an anti-recombinase. Meanwhile, in Drosophila, DmBlm is required specifically to promote the synthesis-dependent strand anneal (SDSA), a type of HR not associating with cross-over. However, conservation of Blm function in SDSA through higher eukaryotes has been a matter of debate. Here, we demonstrate the function of Blm in SDSA type HR in chicken DT40 B lymphocyte line, where Ig gene conversion diversifies the immunoglobulin V gene through intragenic HR between diverged homologous segments. This reaction is initiated by the activation-induced cytidine deaminase enzyme-mediated uracil formation at the V gene, which in turn converts into abasic site, presumably leading to a single strand gap. Ig gene conversion frequency was drastically reduced in BLM−/− cells. In addition, BLM−/− cells used limited donor segments harboring higher identity compared with other segments in Ig gene conversion event, suggesting that Blm can promote HR between diverged sequences. To further understand the role of Blm in HR between diverged homologous sequences, we measured the frequency of gene targeting induced by an I-SceI-endonuclease-mediated double-strand break. BLM−/− cells showed a severer defect in the gene targeting frequency as the number of heterologous sequences increased at the double-strand break site. Conversely, the overexpression of Blm, even an ATPase-defective mutant, strongly stimulated gene targeting. In summary, Blm promotes HR between diverged sequences through a novel ATPase-independent mechanism. PMID:19661064

The Ashkenazic Jewish Bloom syndrome mutation blmAsh is present in non-Jewish Americans of Spanish ancestry.

PubMed Central

Ellis, N A; Ciocci, S; Proytcheva, M; Lennon, D; Groden, J; German, J

1998-01-01

Bloom syndrome (BS) is more frequent in the Ashkenazic Jewish population than in any other. There the predominant mutation, referred to as "blmAsh," is a 6-bp deletion and 7-bp insertion at nucleotide position 2281 in the BLM cDNA. Using a convenient PCR assay, we have identified blmAsh on 58 of 60 chromosomes transmitted by Ashkenazic parents to persons with BS. In contrast, in 91 unrelated non-Ashkenazic persons with BS whom we examined, blmAsh was identified only in 5, these coming from Spanish-speaking Christian families from the southwestern United States, Mexico, or El Salvador. These data, along with haplotype analyses, show that blmAsh was independently established through a founder effect in Ashkenazic Jews and in immigrants to formerly Spanish colonies. This striking observation underscores the complexity of Jewish history and demonstrates the importance of migration and genetic drift in the formation of human populations. PMID:9837821

Light-Scattering Characteristics of Optical Surfaces

DTIC Science & Technology

1975-01-01

6RSTR--O _____4. TITLE (ad . TYPE OF REPORT & PERIOD COVERED 4 Light-Scattering Characteristics of tica: / __ , o -, Sufcs 6 . PERFORMING ORG. REPORT...UO(-90 JJf A0(ct,B;O) e 2r( 6 +Pdd (2) A (cc, 8;A) - JJ U(.t,9;2) e~i2w(+O) dtdg (3) = fJ A(ao;) e i21r(a+00dade. ( 4 ) -c Equations (2) and ( 4 ...Eq. ( 4 ) and requiring the individual plane wave components to satisfy the Helmholtz equation, we find A(ca,;A) - Ao(Ca,;0) e 2wy ( 6 ) where • r. The

Application of Biotic Ligand and Toxic Unit modeling approaches to predict improvements in zooplankton species richness in smelter-damaged lakes near Sudbury, Ontario.

PubMed

Khan, Farhan R; Keller, W Bill; Yan, Norman D; Welsh, Paul G; Wood, Chris M; McGeer, James C

2012-02-07

Using a 30-year record of biological and water chemistry data collected from seven lakes near smelters in Sudbury (Ontario, Canada) we examined the link between reductions of Cu, Ni, and Zn concentrations and zooplankton species richness. The toxicity of the metal mixtures was assessed using an additive Toxic Unit (TU) approach. Four TU models were developed based on total metal concentrations (TM-TU); free ion concentrations (FI-TU); acute LC50s calculated from the Biotic Ligand Model (BLM-TU); and chronic LC50s (acute LC50s adjusted by metal-specific acute-to-chronic ratios, cBLM-TU). All models significantly correlated reductions in metal concentrations to increased zooplankton species richness over time (p < 0.01) with a rank based on r(2) values of cBLM-TU > BLM-TU = FI-TU > TM-TU. Lake-wise comparisons within each model showed that the BLM-TU and cBLM-TU models provided the best description of recovery across all seven lakes. These two models were used to calculate thresholds for chemical and biological recovery using data from reference lakes in the same region. A threshold value of TU = 1 derived from the cBLM-TU provided the most accurate description of recovery. Overall, BLM-based TU models that integrate site-specific water chemistry-derived estimates of toxicity offer a useful predictor of biological recovery.

Stream and River Condition Across the BLM's National System of Public Lands

EPA Science Inventory

Meeting Abstract: The Bureau of Land Management (BLM), in collaboration with the U.S. Environmental Protection Agency, conducted the first ever Western Rivers and Streams Assessment (WRSA), a survey of the condition of BLM streams and rivers throughout the contiguous western U.S...

43 CFR 2802.11 - How does BLM designate corridors?

Code of Federal Regulations, 2013 CFR

2013-10-01

... air, water, soil, fish, wildlife, and vegetation; (3) Physical effects and constraints on corridor... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How does BLM designate corridors? 2802.11... POLICY MANAGEMENT ACT Lands Available for FLPMA Grants § 2802.11 How does BLM designate corridors? (a...

43 CFR 2802.11 - How does BLM designate corridors?

Code of Federal Regulations, 2012 CFR

2012-10-01

... air, water, soil, fish, wildlife, and vegetation; (3) Physical effects and constraints on corridor... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How does BLM designate corridors? 2802.11... POLICY MANAGEMENT ACT Lands Available for FLPMA Grants § 2802.11 How does BLM designate corridors? (a...

43 CFR 2802.11 - How does BLM designate corridors?

Code of Federal Regulations, 2011 CFR

2011-10-01

... air, water, soil, fish, wildlife, and vegetation; (3) Physical effects and constraints on corridor... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How does BLM designate corridors? 2802.11... POLICY MANAGEMENT ACT Lands Available for FLPMA Grants § 2802.11 How does BLM designate corridors? (a...

76 FR 30739 - Notice of Public Meeting, Salem District Resource Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-26

.... ADDRESSES: BLM Salem District Office, 1717 Fabry Road, SE., Salem, OR 97306. FOR FURTHER INFORMATION CONTACT... Hatfield, BLM Salem District Designated Official, 1717 Fabry Road, SE., Salem, OR 97306--(503) 375-5682... quality, land health, forest ecosystems, and infrastructure. Miles R. Brown, BLM Salem District Manager...

78 FR 33103 - Call For Nominations and Comments for the 2013 National Petroleum Reserve in Alaska Oil and Gas...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-03

... available areas is online at http://www.blm.gov/ak . DATES: BLM-Alaska must receive all nominations and...-Alaska Web site at http://www.blm.gov/ak . Authority: 43 CFR 3131.2. Bud Cribley, State Director. [FR Doc...

Stream and River Condition Across the BLM's National System of Public Lands.

EPA Science Inventory

The Bureau of Land Management (BLM), in collaboration with the U.S. Environmental Protection Agency, conducted the first ever Western Rivers and Streams Assessment (WRSA), a survey of the condition of BLM streams and rivers throughout the contiguous western U.S. The WRSA was desi...

78 FR 35956 - Utah Resource Advisory Council Subgroup Conference Call

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-14

... BLM-Utah's draft three-year National Conservation Lands Strategy. In May 2013, the RAC provided the BLM-Utah State Director with recommended changes to the draft strategy and this meeting was held to discuss how BLM-Utah has incorporated their recommendations into a revised draft strategy. A public...

43 CFR 3602.42 - How does BLM publicize competitive mineral materials sales?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How does BLM publicize competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.42 How does BLM publicize competitive...

43 CFR 3602.42 - How does BLM publicize competitive mineral materials sales?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How does BLM publicize competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.42 How does BLM publicize competitive...

43 CFR 3602.43 - How does BLM conduct competitive mineral materials sales?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How does BLM conduct competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.43 How does BLM conduct competitive...

43 CFR 3602.42 - How does BLM publicize competitive mineral materials sales?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How does BLM publicize competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.42 How does BLM publicize competitive...

43 CFR 3602.43 - How does BLM conduct competitive mineral materials sales?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How does BLM conduct competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.43 How does BLM conduct competitive...

43 CFR 3602.43 - How does BLM conduct competitive mineral materials sales?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How does BLM conduct competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.43 How does BLM conduct competitive...

43 CFR 3602.43 - How does BLM conduct competitive mineral materials sales?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How does BLM conduct competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.43 How does BLM conduct competitive...

78 FR 32240 - Notice of Inent (NOI) To Prepare an Environmental Impact Statement (EIS) for the Oro Verde Solar...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-29

... additional alternatives. Alternative A includes full-scale project development of a 450 Megawatt solar PV... the Oro Verde Solar Project at Edwards Air Force Base and County of Kern, CA AGENCY: Department of the... with the development of the Oro Verde Solar Project (OVSP) on Edwards AFB. The OVSP is a solar...

76 FR 66079 - Notice of Intent To Prepare a Supplemental Draft Environmental Impact Statement for the K Road...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-25

.../barstow/K Road_ Calico_Solar.html. E-mail: CalicoPV[email protected] . Fax: (760) 252-6098. Mail: Bureau of... Calico Solar Project, San Bernardino County, CA AGENCY: Bureau of Land Management, Interior. ACTION... Statement (EIS) for an amendment to the right-of- way (ROW) grant for the K Road Calico Solar Project...

SELF-BLM: Prediction of drug-target interactions via self-training SVM.

PubMed

Keum, Jongsoo; Nam, Hojung

2017-01-01

Predicting drug-target interactions is important for the development of novel drugs and the repositioning of drugs. To predict such interactions, there are a number of methods based on drug and target protein similarity. Although these methods, such as the bipartite local model (BLM), show promise, they often categorize unknown interactions as negative interaction. Therefore, these methods are not ideal for finding potential drug-target interactions that have not yet been validated as positive interactions. Thus, here we propose a method that integrates machine learning techniques, such as self-training support vector machine (SVM) and BLM, to develop a self-training bipartite local model (SELF-BLM) that facilitates the identification of potential interactions. The method first categorizes unlabeled interactions and negative interactions among unknown interactions using a clustering method. Then, using the BLM method and self-training SVM, the unlabeled interactions are self-trained and final local classification models are constructed. When applied to four classes of proteins that include enzymes, G-protein coupled receptors (GPCRs), ion channels, and nuclear receptors, SELF-BLM showed the best performance for predicting not only known interactions but also potential interactions in three protein classes compare to other related studies. The implemented software and supporting data are available at https://github.com/GIST-CSBL/SELF-BLM.

Characterization of Bleomycin-Mediated Cleavage of a Hairpin DNA Library

PubMed Central

Segerman, Zachary J.; Roy, Basab; Hecht, Sidney M.

2013-01-01

A study of BLM A5 was conducted using a previously isolated library of hairpin DNAs found to bind strongly to metal free BLM. The ability of Fe(II)•BLM to effect cleavage on both the 3' and 5'-arms of the hairpin DNAs was characterized. The strongly bound DNAs were found to be efficient substrates for Fe•BLM A5-mediated hairpin DNA cleavage. Surprisingly, the most prevalent site of BLM-mediated cleavage was found to be the 5′-AT-3′ dinucleotide sequence. This dinucleotide sequence, and other sequences generally not cleaved well by BLM when examined using arbitrarily chosen DNA substrates, were apparent when examining the library of ten hairpin DNAs. In total, 132 sites of DNA cleavage were produced by exposure of the hairpin DNA library to Fe•BLM A5. The existence of multiple sites of cleavage on both the 3′- and 5′-arms of the hairpin DNAs suggested that some of these might be double-strand cleavage events. Accordingly, an assay was developed with which to test the propensity of the hairpin DNAs to undergo double-strand DNA damage. One hairpin DNA was characterized using this method, and gave results consistent with earlier reports of double-strand DNA cleavage, but with a sequence selectivity different from those reported previously. PMID:23834496

Protectiveness of water quality criteria for copper in western United States waters relative to predicted olfactory responses in juvenile Pacific salmon.

PubMed

DeForest, David K; Gensemer, Robert W; Van Genderen, Eric J; Gorsuch, Joseph W

2011-07-01

Copper (Cu) can impair olfaction in juvenile Pacific salmon (as well as other fishes), thus potentially inhibiting the ability of juveniles to avoid predators or to find food. Because Cu is commonly elevated in stormwater runoff in urban environments, storm events may result in elevated Cu concentrations in salmon-bearing streams. Accordingly, there is concern that existing Cu criteria, which were not derived using data for olfactory-related endpoints, may not be adequately protective of juvenile salmon. However, a modification of the US Environmental Protection Agency (USEPA) biotic ligand model (BLM) for deriving site-specific Cu criteria was recently proposed, which accounted for the sensitivity of olfactory endpoints. The modification was based on olfactory inhibition in juvenile coho salmon (Oncorhynchus kisutch) exposed to Cu in various combinations of pH, hardness, alkalinity, and dissolved organic carbon (DOC) concentrations. We used that olfactory-based BLM to derive 20% inhibition concentrations (IC20) values for Cu for 133 stream locations in the western United States. The olfactory BLM-based IC20 values were compared to the existing hardness-based Cu criteria and the USEPA's BLM-based Cu criteria for these representative natural waters of the western United States. Of the 133 sampling locations, mean hardness-dependent acute and chronic Cu criteria were below the mean olfactory-based BLM IC20 value in 122 (92%) and 129 (97%) of the waters, respectively (i.e., <20% olfactory impairment would have been predicted at the mean hardness-based Cu criteria concentrations). Waters characterized by a combination of high hardness and very low DOC were most likely to have hardness-based Cu criteria that were higher than the olfactory-based BLM IC20 values, because DOC strongly influences Cu bioavailability in the BLM. In all waters, the USEPA's current BLM-based criteria were below the mean olfactory-based BLM IC20 values, indicating that the USEPA's BLM-based criteria are protective of olfactory impairment in juvenile salmon. Copyright © 2011 SETAC.

Development of breast tumors in CHEK2, NBN/NBS1 and BLM mutation carriers does not commonly involve somatic inactivation of the wild-type allele.

PubMed

Suspitsin, Evgeny N; Yanus, Grigory A; Sokolenko, Anna P; Yatsuk, Olga S; Zaitseva, Olga A; Bessonov, Alexandr A; Ivantsov, Alexandr O; Heinstein, Valeria A; Klimashevskiy, Valery F; Togo, Alexandr V; Imyanitov, Evgeny N

2014-02-01

Somatic inactivation of the remaining allele is a characteristic feature of cancers arising in BRCA1 and BRCA2 mutation carriers, which determines their unprecedented sensitivity to some DNA-damaging agents. Data on tumor-specific status of the involved gene in novel varieties of hereditary breast cancer (BC) remain incomplete. We analyzed 32 tumors obtained from 30 patients with non-BRCA1/2 BC-associated germ-line mutations: 25 women were single mutation carriers (7 BLM, 15 CHEK2 and 3 NBN/NBS1) and 5 were double mutation carriers (2 BLM/BRCA1, 1 CHEK2/BLM, 1 CHEK2/BRCA1 and 1 NBN/BLM). Losses of heterozygosity affecting the wild-type allele were detected in none of the tumors from BLM mutation carriers, 3/18 (17 %) CHEK2-associated BC and 1/4 (25 %) NBN/NBS1-driven tumors. The remaining 28 BC were subjected to the sequence analysis of entire coding region of the involved gene; no somatic mutations were identified. We conclude that the tumor-specific loss of the wild-type allele is not characteristic for BC arising in CHEK2, NBN/NBS1 and BLM mutation carriers. Rarity of "second-hit" inactivation of the involved gene in CHEK2-, NBN/NBS1- and BLM-associated BC demonstrates their substantial biological difference from BRCA1/2-driven cancers and makes them poorly suitable for the clinical trials with cisplatin and PARP inhibitors.

43 CFR 2804.25 - How will BLM process my application?

Code of Federal Regulations, 2013 CFR

2013-10-01

... cultural resource surveys or inventories for threatened or endangered species. If BLM needs more... FEDERAL LAND POLICY MANAGEMENT ACT Applying for FLPMA Grants § 2804.25 How will BLM process my application... applicable Federal and state laws; (3) If your application is for a road, determine whether it is in the...

43 CFR 2804.25 - How will BLM process my application?

Code of Federal Regulations, 2014 CFR

2014-10-01

... cultural resource surveys or inventories for threatened or endangered species. If BLM needs more... FEDERAL LAND POLICY MANAGEMENT ACT Applying for FLPMA Grants § 2804.25 How will BLM process my application... applicable Federal and state laws; (3) If your application is for a road, determine whether it is in the...

ACHP | News

Science.gov Websites

the agreement will be signed in February 2012. This final draft of the PA enhances the role of tribal governments, consulting parties, and the public in the BLM project review process. It discusses the role of jcordova@blm.gov. For all other questions regarding the PA, BLM's Preservation Officer Robin Hawks should

Facilitating Backcountry Use of Bureau of Land Management Wildlands

Treesearch

R. Steve Smith

1992-01-01

BLM wildlands are extensive areas which offer opportunities for increased backcountry use. Many BLM wildland areas are not currently receiving much backcountry use due to their unfamiliarity by the public and lack of facilities. Increased urban/BLM wildland interfacing can produce important benefits for both individuals and our society. Using various informational...

43 CFR 3503.41 - Will BLM disclose information I submit under these regulations?

Code of Federal Regulations, 2012 CFR

2012-10-01

... Department of the Interior covering public disclosure of data and information contained in Department of the Interior records. BLM may make certain mineral information not protected from disclosure under part 2 of... § 3503.41 Will BLM disclose information I submit under these regulations? All Federal and Indian data and...

43 CFR 3503.41 - Will BLM disclose information I submit under these regulations?

Code of Federal Regulations, 2013 CFR

2013-10-01

... Department of the Interior covering public disclosure of data and information contained in Department of the Interior records. BLM may make certain mineral information not protected from disclosure under part 2 of... § 3503.41 Will BLM disclose information I submit under these regulations? All Federal and Indian data and...

43 CFR 3505.61 - May BLM extend the term of my prospecting permit?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false May BLM extend the term of my prospecting....61 May BLM extend the term of my prospecting permit? We may extend prospecting permits for phosphate... additional 2 years. We cannot extend sodium and sulphur prospecting permits. ...

43 CFR 3505.61 - May BLM extend the term of my prospecting permit?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false May BLM extend the term of my prospecting....61 May BLM extend the term of my prospecting permit? We may extend prospecting permits for phosphate... additional 2 years. We cannot extend sodium and sulphur prospecting permits. ...

43 CFR 3505.61 - May BLM extend the term of my prospecting permit?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false May BLM extend the term of my prospecting....61 May BLM extend the term of my prospecting permit? We may extend prospecting permits for phosphate... additional 2 years. We cannot extend sodium and sulphur prospecting permits. ...

ACHP | News | BLM Nationwide Programmatic Agreement Addendum Signed

Science.gov Websites

, determined it should be updated to incorporate further the role of Indian tribes. Through this addendum BLM committed to developing revisions to the PA, in consultation with the signatories and Indian tribes, within developing subsequent implementing actions. The addendum also recognizes that the BLM initiated an outreach

43 CFR 1822.11 - What must I do to make an official filing with BLM?

Code of Federal Regulations, 2011 CFR

2011-10-01

... file your application and any other required documents during regular office hours at the appropriate BLM office having jurisdiction over the lands or records involved. You must file any document with BLM through personal delivery or by mailing via the United States Postal Service or other delivery service...

43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral materials...

43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral materials...

43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral materials...

43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral materials...

Bloom syndrome helicase in meiosis: Pro-crossover functions of an anti-crossover protein.

PubMed

Hatkevich, Talia; Sekelsky, Jeff

2017-09-01

The functions of the Bloom syndrome helicase (BLM) and its orthologs are well characterized in mitotic DNA damage repair, but their roles within the context of meiotic recombination are less clear. In meiotic recombination, multiple repair pathways are used to repair meiotic DSBs, and current studies suggest that BLM may regulate the use of these pathways. Based on literature from Saccharomyces cerevisiae, Arabidopsis thaliana, Mus musculus, Drosophila melanogaster, and Caenorhabditis elegans, we present a unified model for a critical meiotic role of BLM and its orthologs. In this model, BLM and its orthologs utilize helicase activity to regulate the use of various pathways in meiotic recombination by continuously disassembling recombination intermediates. This unwinding activity provides the meiotic program with a steady pool of early recombination substrates, increasing the probability for a DSB to be processed by the appropriate pathway. As a result of BLM activity, crossovers are properly placed throughout the genome, promoting proper chromosomal disjunction at the end of meiosis. This unified model can be used to further refine the complex role of BLM and its orthologs in meiotic recombination. © 2017 WILEY Periodicals, Inc.

BLM helicase facilitates telomere replication during leading strand synthesis of telomeres

PubMed Central

Kosiyatrakul, Settapong T.

2015-01-01

Based on its in vitro unwinding activity on G-quadruplex (G4) DNA, the Bloom syndrome–associated helicase BLM is proposed to participate in telomere replication by aiding fork progression through G-rich telomeric DNA. Single molecule analysis of replicated DNA (SMARD) was used to determine the contribution of BLM helicase to telomere replication. In BLM-deficient cells, replication forks initiating from origins within the telomere, which copy the G-rich strand by leading strand synthesis, moved slower through the telomere compared with the adjacent subtelomere. Fork progression through the telomere was further slowed in the presence of a G4 stabilizer. Using a G4-specific antibody, we found that deficiency of BLM, or another G4-unwinding helicase, the Werner syndrome-associated helicase WRN, resulted in increased G4 structures in cells. Importantly, deficiency of either helicase led to greater increases in G4 DNA detected in the telomere compared with G4 seen genome-wide. Collectively, our findings are consistent with BLM helicase facilitating telomere replication by resolving G4 structures formed during copying of the G-rich strand by leading strand synthesis. PMID:26195664

BLM and the FANC proteins collaborate in a common pathway in response to stalled replication forks

PubMed Central

Pichierri, Pietro; Franchitto, Annapaola; Rosselli, Filippo

2004-01-01

Fanconi anaemia (FA) and Bloom syndrome (BS) are autosomal recessive diseases characterised by chromosome fragility and cancer proneness. Here, we report that BLM and the FA pathway are activated in response to both crosslinked DNA and replication fork stall. We provide evidence that BLM and FANCD2 colocalise and co-immunoprecipitate following treatment with either DNA crosslinkers or agents inducing replication arrest. We also find that the FA core complex is necessary for BLM phosphorylation and assembly in nuclear foci in response to crosslinked DNA. Moreover, we show that knock-down of the MRE11 complex, whose function is also under the control of the FA core complex, enhances cellular and chromosomal sensitivity to DNA interstrand crosslinks in BS cells. These findings suggest the existence of a functional link between BLM and the FA pathway and that BLM and the MRE11 complex are in two separated branches of a pathway resulting in S-phase checkpoint activation, chromosome integrity and cell survival in response to crosslinked DNA. PMID:15257300

Improving the corrosion resistance of Mg-4.0Zn-0.2Ca alloy by micro-arc oxidation.

PubMed

Xia, Y H; Zhang, B P; Lu, C X; Geng, L

2013-12-01

In this paper, corrosion resistance of the Mg-4.0Zn-0.2Ca alloy was modified by micro-arc oxidation (MAO) process. The microstructure and phase constituents of MAO layer were characterized by SEM, XRD and X-ray photoelectron spectroscopy (XPS). The corrosion resistance of MAO treated Mg-4.0Zn-0.2Ca alloy in the simulated body fluid were characterized by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) techniques. The microstructure results indicated that a kind of ceramic film was composed by MgO and MgF2 was formed on the surface of Mg-4.0Zn-0.2Ca alloy after MAO treatment. The electrochemical test reveals that the corrosion resistance of MAO treated samples increase 1 order of magnitude. The mechanical intensity test showed that the MAO treated samples has suitable mechanical properties. © 2013.

75 FR 73123 - Notice of Correction to Notice of Availability of the Environmental Assessment and Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-29

...The Bureau of Land Management (BLM) is correcting the Notice of Availability of the Environmental Assessment (EA) and Notice of Public Hearing for the Sage Creek Holdings, LLC, Federal Coal Lease Application, COC-74219 published in the Federal Register on August 13, 2010 [75 FR 49512]. The BLM incorrectly stated that the EA was complete and available for release. The BLM subsequently determined that additional information should have been included in the cumulative impact section of the EA. The BLM will be issuing a revised EA, which will be available for a 30-day comment period upon completion. After the end of the comment period, the BLM will hold a public hearing on the EA, the fair market value and the maximum economic recovery of the proposed leased tract.

Black Lipid Membranes at Bifaces

PubMed Central

Ti Tien, H.

1968-01-01

Black lipid membranes (BLM) less than 90 A thick have been shown to be the most realistic approach to biological membrane models. This paper describes the formation characteristics, optical properties, and thermodynamics of BLM at water/oil/water bifaces. In particular, the nature of the Plateau-Gibbs border which supports the black membrane is analyzed in some detail. The formation of BLM at the biface involves a spontaneous reduction of the free energy of the system. As long as the integrity of the membrane is maintained, the limiting structure of the BLM represents the lowest free energy configuration. PMID:19873618

[Effect of CsA bleomycin-induced interstitial pulmonary disease in mice].

PubMed

Ren, Ying; Yang, Hui; Zhu, Ping; Fan, Chun-mei; Wang, Yan-hong; Li, Jia; Liu, Hui

2012-03-01

To observe the therapeutic effect of cyclosporine A (CsA) on bleomycin (BLM) induced pulmonary fibrosis and to investigate its mechanism. One hundred and twenty C57BL/6 female mice were divided randomly into five groups: BLM model group, control saline group, CsA30 mg treatment group, CsA50 mg treatment group and control treatment group. Treatment groups and model groups were administrated BLM intratracheally to induce interstitial pulmonary disease model, with control saline group administrated with equal volume of normal saline instead. Mice in treatment groups were intraperitoneal injected with CsA, while control treatment group were injected with equal volume of normal saline instead. On the 4th, 7th and 14th day after administration, 8 mice of each group were sacrificed, and the peripheral blood was obtained to count total leucocytes with counting chamber and quantify CD4(+); T cells, CD14(+); monocytes and CD19(+); B cells by flow cytometry (FCM). Bronchoalveolar levage fluid was harvested for cell counting and Giemsa staining. Lung tissues were harvested for immunohistochemical staining and pathological examination. The quantity of total leucocyte was higher in BLM model group than those in control saline group.The proportion of CD14(+); T cells and CD19(+);B cells in BLM model group were increased markedly than those in control saline group on the 4th, 7th and 14th day post BLM. With CsA treatment, The proportion of CD14(+); T cells was lower than BLM model group at the same time point, especially on the 4th day. The proportion of CD19(+); B cells were significantly lower than those of BLM model group at the same time point(7 d, 14 d). The total and classification of cells of BLM model group were increased markedly than those in control saline group, and decreased obviously in the treatment groups at the same time point. Examination of lung tissues: With the prolonged time of BLM administration, it showed wider alveolar septum, more collagen deposition, as well as more infiltrating inflammatory cells which consisted of generous lymphocyte and few mononuclear macrophages than those in saline control group. With the prolonged time of CsA injection, the interstitial pulmonary inflammation was remissive, and there was less fibroblast infiltration and collagen deposition in pulmonary interstitium and periphery of bronchiole. Alveolar epithelial cells, bronchiolar epithelial cells, mononuclear macrophages, neutrophils and lymphocytes were demonstrated to express CD147, there was higher CD147 expression in BLM model group than those in CsA treatment groups. CsA may heal BLM induced interstitial pulmonary disease by blocking CD147-CypA interaction, then decreasing chemotaxis for the immunocyte, and reducing migration of immunocytes to the lung and collagen deposition in the lung.

Accurate and rapid modeling of iron-bleomycin-induced DNA damage using tethered duplex oligonucleotides and electrospray ionization ion trap mass spectrometric analysis.

PubMed

Harsch, A; Marzilli, L A; Bunt, R C; Stubbe, J; Vouros, P

2000-05-01

Bleomycin B(2)(BLM) in the presence of iron [Fe(II)] and O(2)catalyzes single-stranded (ss) and double-stranded (ds) cleavage of DNA. Electrospray ionization ion trap mass spectrometry was used to monitor these cleavage processes. Two duplex oligonucleotides containing an ethylene oxide tether between both strands were used in this investigation, allowing facile monitoring of all ss and ds cleavage events. A sequence for site-specific binding and cleavage by Fe-BLM was incorporated into each analyte. One of these core sequences, GTAC, is a known hot-spot for ds cleavage, while the other sequence, GGCC, is a hot-spot for ss cleavage. Incubation of each oligo-nucleotide under anaerobic conditions with Fe(II)-BLM allowed detection of the non-covalent ternary Fe-BLM/oligonucleotide complex in the gas phase. Cleavage studies were then performed utilizing O(2)-activated Fe(II)-BLM. No work-up or separation steps were required and direct MS and MS/MS analyses of the crude reaction mixtures confirmed sequence-specific Fe-BLM-induced cleavage. Comparison of the cleavage patterns for both oligonucleotides revealed sequence-dependent preferences for ss and ds cleavages in accordance with previously established gel electrophoresis analysis of hairpin oligonucleotides. This novel methodology allowed direct, rapid and accurate determination of cleavage profiles of model duplex oligonucleotides after exposure to activated Fe-BLM.

43 CFR 2804.25 - How will BLM process my application?

Code of Federal Regulations, 2012 CFR

2012-10-01

... cultural resource surveys or inventories for threatened or endangered species. If BLM needs more... the General Mining Law, but not from the Mineral Leasing Act of 1920 (30 U.S.C. 181 et seq.) or the... FEDERAL LAND POLICY MANAGEMENT ACT Applying for FLPMA Grants § 2804.25 How will BLM process my application...

43 CFR 2804.25 - How will BLM process my application?

Code of Federal Regulations, 2011 CFR

2011-10-01

... cultural resource surveys or inventories for threatened or endangered species. If BLM needs more... the General Mining Law, but not from the Mineral Leasing Act of 1920 (30 U.S.C. 181 et seq.) or the... FEDERAL LAND POLICY MANAGEMENT ACT Applying for FLPMA Grants § 2804.25 How will BLM process my application...

43 CFR 3602.14 - What kind of financial security does BLM require?

Code of Federal Regulations, 2011 CFR

2011-10-01

... DISPOSAL Mineral Materials Sales Applications § 3602.14 What kind of financial security does BLM require? (a) For contracts of $2,000 or more, BLM will require a performance bond of an amount sufficient to meet the reclamation standards provided for in the contract, but at least $500. If you have a sales...

75 FR 26986 - Notice of Intent To Prepare an Amendment to the Kobuk-Seward Peninsula Resource Management Plan...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-13

... Conservation Act of 1980 (ANILCA), and the BLM policies. The BLM will work collaboratively with interested...: The BLM-administered Squirrel River SRMA is located in western Alaska, approximately 30 miles... amendment and associated EA will meet the requirements of the National Environmental Policy Act of 1969...

43 CFR 2884.22 - Can BLM ask me for additional information?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Can BLM ask me for additional information? 2884.22 Section 2884.22 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU... THE MINERAL LEASING ACT Applying for MLA Grants or TUPs § 2884.22 Can BLM ask me for additional...

43 CFR 2884.22 - Can BLM ask me for additional information?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Can BLM ask me for additional information? 2884.22 Section 2884.22 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU... THE MINERAL LEASING ACT Applying for MLA Grants or TUPs § 2884.22 Can BLM ask me for additional...

43 CFR 3809.202 - Under what conditions will BLM defer to State regulation of operations?

Code of Federal Regulations, 2013 CFR

2013-10-01

... standards on a provision-by-provision basis to determine— (i) Whether non-numerical State standards are functionally equivalent to BLM counterparts; and (ii) Whether numerical State standards are the same as corresponding numerical BLM standards, except that State review and approval time frames do not have to be the...

43 CFR 3809.202 - Under what conditions will BLM defer to State regulation of operations?

Code of Federal Regulations, 2012 CFR

2012-10-01

... standards on a provision-by-provision basis to determine— (i) Whether non-numerical State standards are functionally equivalent to BLM counterparts; and (ii) Whether numerical State standards are the same as corresponding numerical BLM standards, except that State review and approval time frames do not have to be the...

43 CFR 3809.202 - Under what conditions will BLM defer to State regulation of operations?

Code of Federal Regulations, 2011 CFR

2011-10-01

... standards on a provision-by-provision basis to determine— (i) Whether non-numerical State standards are functionally equivalent to BLM counterparts; and (ii) Whether numerical State standards are the same as corresponding numerical BLM standards, except that State review and approval time frames do not have to be the...

43 CFR 3809.202 - Under what conditions will BLM defer to State regulation of operations?

Code of Federal Regulations, 2014 CFR

2014-10-01

... standards on a provision-by-provision basis to determine— (i) Whether non-numerical State standards are functionally equivalent to BLM counterparts; and (ii) Whether numerical State standards are the same as corresponding numerical BLM standards, except that State review and approval time frames do not have to be the...

43 CFR 2806.43 - How does BLM calculate rent for passive reflectors and local exchange networks?

Code of Federal Regulations, 2011 CFR

2011-10-01

... reflectors and local exchange networks? 2806.43 Section 2806.43 Public Lands: Interior Regulations Relating...-Of-Way § 2806.43 How does BLM calculate rent for passive reflectors and local exchange networks? (a) BLM calculates rent for passive reflectors and local exchange networks by using the same rent...

43 CFR 3602.27 - When will BLM extend the term of a contract?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false When will BLM extend the term of a contract? 3602.27 Section 3602.27 Public Lands: Interior Regulations Relating to Public Lands (Continued... DISPOSAL Mineral Materials Sales Administration of Sales § 3602.27 When will BLM extend the term of a...

43 CFR 3505.62 - Under what conditions will BLM extend my prospecting permit?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Under what conditions will BLM extend my prospecting permit? 3505.62 Section 3505.62 Public Lands: Interior Regulations Relating to Public Lands....62 Under what conditions will BLM extend my prospecting permit? You must prove that: (a) You explored...

43 CFR 3602.27 - When will BLM extend the term of a contract?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false When will BLM extend the term of a contract? 3602.27 Section 3602.27 Public Lands: Interior Regulations Relating to Public Lands (Continued... DISPOSAL Mineral Materials Sales Administration of Sales § 3602.27 When will BLM extend the term of a...

43 CFR 3505.62 - Under what conditions will BLM extend my prospecting permit?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Under what conditions will BLM extend my prospecting permit? 3505.62 Section 3505.62 Public Lands: Interior Regulations Relating to Public Lands....62 Under what conditions will BLM extend my prospecting permit? You must prove that: (a) You explored...

43 CFR 2884.11 - What information must I submit in my application?

Code of Federal Regulations, 2012 CFR

2012-10-01

... application? (a) File your application on Form SF-299 or as part of an Application for Permit to Drill or Reenter (BLM Form 3160-3) or Sundry Notice and Report on Wells (BLM Form 3160-5), available from any BLM... agencies, such as the Federal Energy Regulatory Commission (see 18 CFR chapter I), for licenses...

43 CFR 2884.11 - What information must I submit in my application?

Code of Federal Regulations, 2013 CFR

2013-10-01

... application? (a) File your application on Form SF-299 or as part of an Application for Permit to Drill or Reenter (BLM Form 3160-3) or Sundry Notice and Report on Wells (BLM Form 3160-5), available from any BLM... agencies, such as the Federal Energy Regulatory Commission (see 18 CFR chapter I), for licenses...

43 CFR 2884.11 - What information must I submit in my application?

Code of Federal Regulations, 2011 CFR

2011-10-01

... application? (a) File your application on Form SF-299 or as part of an Application for Permit to Drill or Reenter (BLM Form 3160-3) or Sundry Notice and Report on Wells (BLM Form 3160-5), available from any BLM... agencies, such as the Federal Energy Regulatory Commission (see 18 CFR chapter I), for licenses...

43 CFR 2884.11 - What information must I submit in my application?

Code of Federal Regulations, 2014 CFR

2014-10-01

... application? (a) File your application on Form SF-299 or as part of an Application for Permit to Drill or Reenter (BLM Form 3160-3) or Sundry Notice and Report on Wells (BLM Form 3160-5), available from any BLM... agencies, such as the Federal Energy Regulatory Commission (see 18 CFR chapter I), for licenses...

43 CFR 3264.11 - What must I submit to BLM after I finish subsequent well operations?

Code of Federal Regulations, 2011 CFR

2011-10-01

... subsequent well operations? 3264.11 Section 3264.11 Public Lands: Interior Regulations Relating to Public...) GEOTHERMAL RESOURCE LEASING Reports-Drilling Operations § 3264.11 What must I submit to BLM after I finish subsequent well operations? (a) Submit to BLM a subsequent well operations report within 30 days after...

78 FR 29379 - BLM Director's Response to the Appeal by the Governors of Utah and Wyoming of the BLM Assistant...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-20

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLWO320000 L13100000 DT0000 LXSIOSHL0000... Director's Governor's Consistency Review Determination AGENCY: Bureau of Land Management, Interior. ACTION... Shale and Tar Sands Resources on Lands Administered by the Bureau of Land Management (BLM) in Colorado...

43 CFR 3931.100 - Boundary pillars and buffer zones.

Code of Federal Regulations, 2013 CFR

2013-10-01

... otherwise specified in writing by the BLM. Boundary and other main pillars may be mined only with the BLM's prior written consent or on the BLM's order. For in-situ operations, a 50-foot buffer zone from the..., mine out and remove all available oil shale in such boundary pillar, both in the lands covered by the...

43 CFR 3931.100 - Boundary pillars and buffer zones.

Code of Federal Regulations, 2012 CFR

2012-10-01

... otherwise specified in writing by the BLM. Boundary and other main pillars may be mined only with the BLM's prior written consent or on the BLM's order. For in-situ operations, a 50-foot buffer zone from the..., mine out and remove all available oil shale in such boundary pillar, both in the lands covered by the...

43 CFR 3931.100 - Boundary pillars and buffer zones.

Code of Federal Regulations, 2014 CFR

2014-10-01

... otherwise specified in writing by the BLM. Boundary and other main pillars may be mined only with the BLM's prior written consent or on the BLM's order. For in-situ operations, a 50-foot buffer zone from the..., mine out and remove all available oil shale in such boundary pillar, both in the lands covered by the...

43 CFR 3931.100 - Boundary pillars and buffer zones.

Code of Federal Regulations, 2011 CFR

2011-10-01

... otherwise specified in writing by the BLM. Boundary and other main pillars may be mined only with the BLM's prior written consent or on the BLM's order. For in-situ operations, a 50-foot buffer zone from the..., mine out and remove all available oil shale in such boundary pillar, both in the lands covered by the...

Biotic ligand modeling approach: Synthesis of the effect of major cations on the toxicity of metals to soil and aquatic organisms.

PubMed

Ardestani, Masoud M; van Straalen, Nico M; van Gestel, Cornelis A M

2015-10-01

The biotic ligand model (BLM) approach is used to assess metal toxicity, taking into account the competition of other cations with the free metal ions for binding to the biotic ligand sites of aquatic and soil organisms. The bioavailable fraction of metals, represented by the free metal ion, is a better measure than the total concentration for assessing their potential risk to the environment. Because BLMs are relating toxicity to the fraction of biotic ligands occupied by the metal, they can be useful for investigating factors affecting metal bioaccumulation and toxicity. In the present review, the effects of major cations on the toxicity of metals to soil and aquatic organisms were comprehensively studied by performing a meta-analysis of BLM literature data. Interactions at the binding sites were shown to be species- and metal-specific. The main factors affecting the relationships between toxicity and conditional binding constants for metal binding at the biotic ligand appeared to be Ca(2+) , Mg(2+) , and protons. Other important characteristics of the exposure medium, such as levels of dissolved organic carbon and concentrations of other cations, should also be considered to obtain a proper assessment of metal toxicity to soil and aquatic organisms. © 2015 SETAC.

Changes in expression of cytokines in polyhexamethylene guanidine-induced lung fibrosis in mice: Comparison of bleomycin-induced lung fibrosis.

PubMed

Kim, Min-Seok; Kim, Sung-Hwan; Jeon, Doin; Kim, Hyeon-Young; Lee, Kyuhong

2018-01-15

Inhalation of polyhexamethylene guanidine (PHMG) causes irreversible pulmonary injury, such as pulmonary fibrosis. However, the mechanism underlying PHMG-induced lung injury is unclear. In this study, we compared the difference in time-dependent lung injury between PHMG- and bleomycin (BLM)-treated mice and determined cytokines involved in inducing lung injury by performing cytokine antibody array analysis. Mice were treated once with 1.8mg/kg BLM or 1.2mg/kg PHMG through intratracheal instillation and were sacrificed on days 7 and 28. Bronchoalveolar lavage fluid (BALF) analysis showed that the number of neutrophils was significantly higher in PHMG-treated mice than in BLM-treated mice on day 7. Histopathological analysis showed inflammatory cell infiltration and fibrosis mainly in the terminal bronchioles and alveoli in the lungs of PHMG- and BLM-treated mice. However, continuous macrophage infiltration in the alveolar space and bronchioloalveolar epithelial hyperplasia (BEH) were only observed in PHMG-treated mice. Cytokine antibody array analysis showed that 15 and eight cytokines were upregulated in PHMG- and BLM-treated mice, respectively, on day 7. On day 28, 13 and five cytokines were upregulated in PHMG and BLM-treated mice, respectively. In addition, the expressed cytokines between days 7 and 28 in BLM-treated mice were clearly different, but were similar in PHMG-treated mice. Consequently, between PHMG- and BLM-treated mice, we observed differences in the expression patterns and types of cytokines. These differences are considered to be a result of the inflammatory processes induced by both substances, which may mainly involve macrophage infiltration. Therefore, continuous induction of the inflammatory response by PHMG may play an important role in the development of pulmonary fibrosis. Copyright © 2017 Elsevier B.V. All rights reserved.

Structural basis of Bloom syndrome (BS) causing mutations in the BLM helicase domain.

PubMed Central

Rong, S. B.; Väliaho, J.; Vihinen, M.

2000-01-01

BACKGROUND: Bloom syndrome (BS) is characterized by mutations within the BLM gene. The Bloom syndrome protein (BLM) has similarity to the RecQ subfamily of DNA helicases, which contain seven conserved helicase domains and share significant sequence and structural similarity with the Rep and PcrA DNA helicases. We modeled the three-dimensional structure of the BLM helicase domain to analyze the structural basis of BS-causing mutations. MATERIALS AND METHODS: The sequence alignment was performed for RecQ DNA helicases and Rep and PcrA helicases. The crystal structure of PcrA helicase (PDB entry 3PJR) was used as the template for modeling the BLM helicase domain. The model was used to infer the function of BLM and to analyze the effect of the mutations. RESULTS: The structural model with good stereochemistry of the BLM helicase domain contains two subdomains, 1A and 2A. The electrostatic potential of the model is highly negative over most of the surface, except for the cleft between subdomains 1A and 2A which is similar to the template protein. The ATP-binding site is located inside the model between subdomains 1A and 2A; whereas, the DNA-binding region is situated at the surface cleft, with positive potential between 1A and 2A. CONCLUSIONS: The three-dimensional structure of the BLM helicase domain was modeled and applied to interpret BS-causing mutations. The mutation I841T is likely to weaken DNA binding, while the mutations C891R, C901Y, and Q672R presumably disturb the ATP binding. In addition, other critical positions are discussed. PMID:10965492

77 FR 71822 - Notice of Availability of the BLM's Responses to Public Comments and of the BLM's Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-04

... century. In 1925, potash (potassium-bearing salts primarily used for fertilizer) was discovered in this... is found in formations below the potash-bearing formations, so oil and gas wells must extend through... industries. This conflict has resulted in a great deal of litigation regarding decisions made by the BLM on a...

43 CFR 3508.14 - How will BLM publish the notice of lease sale?

Code of Federal Regulations, 2011 CFR

2011-10-01

... notice of lease sale at least once a week for three consecutive weeks in a newspaper of general circulation in the area where the lands are situated. We will also post the notice of lease sale for 30 days...; (3) A description of the tract BLM is offering; (4) A description of the mineral deposit BLM is...

43 CFR 2808.11 - What will BLM do if it determines that I am in trespass?

Code of Federal Regulations, 2012 CFR

2012-10-01

... am in trespass? 2808.11 Section 2808.11 Public Lands: Interior Regulations Relating to Public Lands...-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Trespass § 2808.11 What will BLM do if it determines that I am in trespass? (a) BLM will notify you in writing of the trespass and explain your liability...

43 CFR 2808.11 - What will BLM do if it determines that I am in trespass?

Code of Federal Regulations, 2014 CFR

2014-10-01

... am in trespass? 2808.11 Section 2808.11 Public Lands: Interior Regulations Relating to Public Lands...-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Trespass § 2808.11 What will BLM do if it determines that I am in trespass? (a) BLM will notify you in writing of the trespass and explain your liability...

43 CFR 2808.11 - What will BLM do if it determines that I am in trespass?

Code of Federal Regulations, 2011 CFR

2011-10-01

... am in trespass? 2808.11 Section 2808.11 Public Lands: Interior Regulations Relating to Public Lands...-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Trespass § 2808.11 What will BLM do if it determines that I am in trespass? (a) BLM will notify you in writing of the trespass and explain your liability...

43 CFR 2808.11 - What will BLM do if it determines that I am in trespass?

Code of Federal Regulations, 2013 CFR

2013-10-01

... am in trespass? 2808.11 Section 2808.11 Public Lands: Interior Regulations Relating to Public Lands...-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Trespass § 2808.11 What will BLM do if it determines that I am in trespass? (a) BLM will notify you in writing of the trespass and explain your liability...

78 FR 8188 - Notice of Realty Action: Proposed (Non-Competitive) Direct Sale of Public Land in Carbon County, UT

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-05

... by March 22, 2013. ADDRESSES: Address all written comments concerning this notice to the BLM, Price Field Office, Attn: Connie Leschin, 125 S. 600 W., Price, Utah, 84501. FOR FURTHER INFORMATION CONTACT... survey of the said land, on file with the BLM. The proposed sale is in conformance with the BLM Price...

A GIS-based method to evaluate undeveloped BLM lands in Alaska

Treesearch

Jason Geck

2007-01-01

As Alaska’s largest land management agency, the Bureau of Land Management (BLM) has responsibility for over 87 million acres (35 million ha) of public lands throughout the state. By using datasets and Landsat scenes within a Geographical Information System (GIS), this study prioritizes wilderness protection through the ranking of BLM blocks (contiguous land parcels),...

43 CFR 3280.5 - May BLM require the modification of lease requirements in connection with the creation and...

Code of Federal Regulations, 2012 CFR

2012-10-01

... requirements in connection with the creation and operation of a unit agreement? 3280.5 Section 3280.5 Public... with the creation and operation of a unit agreement? (a) BLM may, with the consent of the lessees... connection with the creation and operation of any such unit agreement as BLM may consider necessary or...

43 CFR 3280.5 - May BLM require the modification of lease requirements in connection with the creation and...

Code of Federal Regulations, 2013 CFR

2013-10-01

... requirements in connection with the creation and operation of a unit agreement? 3280.5 Section 3280.5 Public... with the creation and operation of a unit agreement? (a) BLM may, with the consent of the lessees... connection with the creation and operation of any such unit agreement as BLM may consider necessary or...

43 CFR 3280.5 - May BLM require the modification of lease requirements in connection with the creation and...

Code of Federal Regulations, 2011 CFR

2011-10-01

... requirements in connection with the creation and operation of a unit agreement? 3280.5 Section 3280.5 Public... with the creation and operation of a unit agreement? (a) BLM may, with the consent of the lessees... connection with the creation and operation of any such unit agreement as BLM may consider necessary or...

43 CFR 3280.5 - May BLM require the modification of lease requirements in connection with the creation and...

Code of Federal Regulations, 2014 CFR

2014-10-01

... requirements in connection with the creation and operation of a unit agreement? 3280.5 Section 3280.5 Public... with the creation and operation of a unit agreement? (a) BLM may, with the consent of the lessees... connection with the creation and operation of any such unit agreement as BLM may consider necessary or...

43 CFR 2806.15 - Under what circumstances may BLM waive or reduce my rent?

Code of Federal Regulations, 2011 CFR

2011-10-01

... qualifies for a waiver or a reduction of rent. (b) BLM may waive or reduce your rent if you show BLM that: (1) You are a non-profit organization, corporation, or association which is not controlled by, or is not a subsidiary of, a profit making corporation or business enterprise and the facility or project...

Bleomycin enhances the efficacy of sonodynamic therapy using aluminum phthalocyanine disulfonate.

PubMed

Osaki, Tomohiro; Yokoe, Inoru; Uto, Yoshihiro; Ishizuka, Masahiro; Tanaka, Toru; Yamanaka, Nobuyasu; Kurahashi, Tsukasa; Azuma, Kazuo; Murahata, Yusuke; Tsuka, Takeshi; Ito, Norihiko; Imagawa, Tomohiro; Okamoto, Yoshiharu

2016-01-01

Sonodynamic therapy (SDT), or ultrasound combined with sonosensitization, is a promising approach because it is noninvasive and penetrates deeper than light does in photodynamic therapy. We examined whether bleomycin (BLM) could improve the efficacy of SDT. We performed an in vitro study using Colon-26 cells, which are derived from mouse colon cancer. SDT with BLM was significantly more cytotoxic than SDT alone both in vitro and in vivo. We also observed an ultrasound intensity-dependent cytotoxic effect of SDT with BLM. These findings suggest that SDT with BLM might provide a novel noninvasive treatment for deep-seated tumors. Copyright © 2015 Elsevier B.V. All rights reserved.

Strain improvement by combined UV mutagenesis and ribosome engineering and subsequent fermentation optimization for enhanced 6'-deoxy-bleomycin Z production.

PubMed

Zhu, Xiangcheng; Kong, Jieqian; Yang, Hu; Huang, Rong; Huang, Yong; Yang, Dong; Shen, Ben; Duan, Yanwen

2018-02-01

The bleomycins (BLMs) are important clinical drugs extensively used in combination chemotherapy for the treatment of various cancers. Dose-dependent lung toxicity and the development of drug resistance have restricted their wide applications. 6'-Deoxy-BLM Z, a recently engineered BLM analogue with improved antitumor activity, has the potential to be developed into the next-generation BLM anticancer drug. However, its low titer in the recombinant strain Streptomyces flavoviridis SB9026 has hampered current efforts, which require sufficient compound, to pursue preclinical studies and subsequent clinical development. Here, we report the strain improvement by combined UV mutagenesis and ribosome engineering, as well as the fermentation optimization, for enhanced 6'-deoxy-BLM production. A high producer, named S. flavoviridis G-4F12, was successfully isolated, producing 6'-deoxy-BLM at above 70 mg/L under the optimized fermentation conditions, representing a sevenfold increase in comparison with that of the original producer. These findings demonstrated the effectiveness of combined empirical breeding methods in strain improvement and set the stage for sustainable production of 6'-deoxy-BLM via pilot-scale microbial fermentation.

Crystal Structures of RMI1 and RMI2, Two OB-Fold Regulatory Subunits of the BLM Complex

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Feng; Yang, Yuting; Singh, Thiyam Ramsing

Mutations in BLM, a RecQ-like helicase, are linked to the autosomal recessive cancer-prone disorder Bloom's syndrome. BLM associates with topoisomerase (Topo) III{alpha}, RMI1, and RMI2 to form the BLM complex that is essential for genome stability. The RMI1-RMI2 heterodimer stimulates the dissolution of double Holliday junction into non-crossover recombinants mediated by BLM-Topo III{alpha} and is essential for stabilizing the BLM complex. However, the molecular basis of these functions of RMI1 and RMI2 remains unclear. Here we report the crystal structures of multiple domains of RMI1-RMI2, providing direct confirmation of the existence of three oligonucleotide/oligosaccharide binding (OB)-folds in RMI1-RMI2. Our structuralmore » and biochemical analyses revealed an unexpected insertion motif in RMI1N-OB, which is important for stimulating the dHJ dissolution. We also revealed the structural basis of the interaction between RMI1C-OB and RMI2-OB and demonstrated the functional importance of the RMI1-RMI2 interaction in genome stability maintenance.« less

Histopathological Correlations between Mediastinal Fat-Associated Lymphoid Clusters and the Development of Lung Inflammation and Fibrosis following Bleomycin Administration in Mice.

PubMed

Elewa, Yaser Hosny Ali; Ichii, Osamu; Takada, Kensuke; Nakamura, Teppei; Masum, Md Abdul; Kon, Yasuhiro

2018-01-01

Bleomycin (BLM) has been reported to induce lung inflammation and fibrosis in human and mice and showed genetic susceptibility. Interestingly, the C57BL/6 (B6) mice had prominent mediastinal fat-associated lymphoid cluster (MFALCs) under healthy condition, and showed susceptibility to development of lung fibrosis following BLM administration. However, the pathogenesis of lung lesion progression, and their correlation with MFALC morphologies, remain to be clarified. To investigate the correlations between MFALC structures and lung injuries in B6 mice, histopathological examination of mediastinal fat tissues and lungs was examined at 7 and 21 days (d) following a single 50 μL intranasal (i.n.) instillation of either BLM sulfate (5 mg/kg) (BLM group) or phosphate-buffered saline (control group). The lung fibrosis was examined by Masson's trichrome (MT) stain of paraffin sections and mRNA expression levels of Col1a1, Col3a1, and Acta2 in different frozen lung samples. Furthermore, immunohistochemistry for CD3, B220, Iba1, Gr1, BrdU, LYVE-1, and peripheral node addressin (PNAd) was performed to detect T- and B-cells, macrophages, granulocytes, proliferating cells, lymph vessels (LVs), and high endothelial venules (HEVs). We found that MFALCs were more abundant in the BLM group as compared to the control group. The lung of BLM group developed pneumonitis with severe cellular infiltrations at 7 days and significant collagen deposition (MT) and higher expression of Col1a1, and Col3a1 at 21 days post-administration. Numerous immune cells, proliferating cells, HEVs, and LVs were observed in both MFALCs and lungs of the BLM group. Interestingly, PNAd + HEVs were observed in the lungs of the BLM group, but not the control group. Moreover, numerous Gr1 + polymorphonuclear and mononuclear-like ring cells were found in the MFALCs and lungs of the BLM group. Interestingly, flow cytometric analysis revealed a significant increase of B-cell populations within the MFALCs of BLM group suggesting a potential proliferative induction of B-cells following inflammation. Furthermore, significant positive correlations were observed between quantitative parameters of these immune cells in both the lungs and MFALCs. Thus, we suggest a potentially important role for MFALCs and HEVs in the progression of lung disease, especially in inflammatory lung disease.

Insitu grown superhydrophobic Zn-Al layered double hydroxides films on magnesium alloy to improve corrosion properties

NASA Astrophysics Data System (ADS)

Zhou, Meng; Pang, Xiaolu; Wei, Liang; Gao, Kewei

2015-05-01

A hierarchical superhydrophobic zinc-aluminum layered double hydroxides (Zn-Al LDHs) film has been fabricated on a magnesium alloy substrate via a facile hydrothermal crystallization method following chemical modification. The characteristics of the films were investigated by X-ray diffraction (XRD), scanning electronic microscope (SEM), and energy dispersive spectroscopy (EDS). XRD patterns and SEM images showed that the micro/nanoscale hierarchical LDHs film surfaces composed of ZnO nanorods and Zn-Al LDHs nanowalls structures. The static contact angle (CA) for the prepared surfaces was observed at around 165.6°. The corrosion resistance of the superhydrophobic films was estimated by electrochemical impedance spectroscopy (EIS) and potentiondynamic polarization measurement. EIS and polarization measurements revealed that the superhydrophobic Zn-Al LDHs coated magnesium alloy had better corrosion resistance in neutral 3.5 wt.% NaCl solution.

Use of the Biotic Ligand Model to predict metal toxicity to aquatic biota in areas of differing geology

USGS Publications Warehouse

Smith, Kathleen S.

2005-01-01

This work evaluates the use of the biotic ligand model (BLM), an aquatic toxicity model, to predict toxic effects of metals on aquatic biota in areas underlain by different rock types. The chemical composition of water, soil, and sediment is largely derived from the composition of the underlying rock. Geologic source materials control key attributes of water chemistry that affect metal toxicity to aquatic biota, including: 1) potentially toxic elements, 2) alkalinity, 3) total dissolved solids, and 4) soluble major elements, such as Ca and Mg, which contribute to water hardness. Miller (2002) compiled chemical data for water samples collected in watersheds underlain by ten different rock types, and in a mineralized area in western Colorado. He found that each rock type has a unique range of water chemistry. In this study, the ten rock types were grouped into two general categories, igneous and sedimentary. Water collected in watersheds underlain by sedimentary rock has higher mean pH, alkalinity, and calcium concentrations than water collected in watersheds underlain by igneous rock. Water collected in the mineralized area had elevated concentrations of calcium and sulfate in addition to other chemical constituents. Miller's water-chemistry data were used in the BLM (computer program) to determine copper and zinc toxicity to Daphnia magna. Modeling results show that waters from watersheds underlain by different rock types have characteristic ranges of predicted LC 50 values (a measurement of aquatic toxicity) for copper and zinc, with watersheds underlain by igneous rock having lower predicted LC 50 values than watersheds underlain by sedimentary rock. Lower predicted LC 50 values suggest that aquatic biota in watersheds underlain by igneous rock may be more vulnerable to copper and zinc inputs than aquatic biota in watersheds underlain by sedimentary rock. For both copper and zinc, there is a trend of increasing predicted LC 50 values with increasing dissolved organic carbon (DOC) concentrations. Predicted copper LC 50 values are extremely sensitive to DOC concentrations, whereas alkalinity appears to have an influence on zinc toxicity at alkalinities in excess of about 100 mg/L CaCO 3 . These findings show promise for coupling the BLM (computer program) with measured water-chemistry data to predict metal toxicity to aquatic biota in different geologic settings and under different scenarios. This approach may ultimately be a useful tool for mine-site planning, mitigation and remediation strategies, and ecological risk assessment.

75 FR 20379 - Notice of Intent To Prepare an Environmental Impact Statement for the Proposed Hollister...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-19

..., the Bureau of Land Management (BLM) Tuscarora Field Office, Elko, Nevada, intends to prepare an..., newspapers and the BLM Web site at: http://www.blm.gov/nv/st/en/fo/elko_field_office.html . In order to be....gov/nv/st/en/fo/elko_field_office.html . E-mail: [email protected] . Fax: (775) 753-0255. Mail...

30 CFR 218.305 - How do I pay advanced royalties I owe under BLM regulations?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 2 2010-07-01 2010-07-01 false How do I pay advanced royalties I owe under BLM... Geothermal Resources § 218.305 How do I pay advanced royalties I owe under BLM regulations? If you pay advanced royalties under 43 CFR 3212.15(a)(1) to retain your lease: (a) You must pay an advanced royalty...

30 CFR 1218.305 - How do I pay advanced royalties I owe under BLM regulations?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 3 2011-07-01 2011-07-01 false How do I pay advanced royalties I owe under BLM... CREDITS AND INCENTIVES Geothermal Resources § 1218.305 How do I pay advanced royalties I owe under BLM regulations? If you pay advanced royalties under 43 CFR 3212.15(a)(1) to retain your lease: (a) You must pay...

75 FR 26786 - Notice of Public Meeting: Sierra Front-Northwestern Great Basin Resource Advisory Council, NV

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-12

...), will meet in Winnemucca, Nevada. The meeting is open to the public. Dates and Times: July 13-14, 2010, at the BLM Winnemucca District Office, 5100 E. Winnemucca Blvd., Winnemucca, Nevada. A field trip to..., locations, field trips and meeting times, will be posted on the BLM Web site at: http://www.blm.gov/nv/st/en...

[Mechanism involving blm gene underlies repair of DNA damage of Jurkat cells induced by mitomycin C].

PubMed

Yi, Xue; Cheng, Hui; Zou, Ping; Liu, Ling-Bo; Zhang, Ting; Yu, Dan; Zhu, Xiao-Ming; Zou, Liang

2010-10-01

The defect or block of apoptosis is an important factor involved in the drug resistance of tumor cells. Blm gene plays a great role in DNA damage and repair. This study was aimed to explore the relationship of blm gene expression with cell cycle and apoptosis after Jurkat DNA damage. The apoptosis rate and change of cell cycle were detected by flow cytometry, the expression level of blm mRNA in Jurkat cells was determined by semi-quantitative RT-PCR. The results indicated that after induction with 0.4 g/L of mitomycin C (MMC) for 24 hours the apoptosis rate of Jurkat cells were (11.42±0.013)%, and (66.08±1.60)% Jurkat cells were arrested in G2/M phase. After induction for 48 hours, the apoptosis rate of Jurkat cells declined from (11.42±0.013)% to (8.08±0.27)%, and cell count of Jurkat cells arrested in G2/M phase decreased from (66.08±1.60)% to (33.96±1.05)%. When induced with 0.4 g/L of MMC for 24 hours, the apoptosis rate of fibroblasts and the percentage of fibroblasts in G2/M, G0-G1 and S phase all showed no significant change until 48 hours. The range of apoptosis rate and the change of cell percentage in three phases were significantly different between Jurkat cells and fibroblasts (p<0.01). Expression level of blm mRNA in Jurkat cells was remarkably higher than that in normal fibroblasts (p<0.01), at 48 hours expression level of blm mRNA was remarkably higher than that at 24 hours. The 2 groups showed clear difference of blm mRNA expression after treated by MMC (p<0.01). It is concluded that the blm gene may play a significant role in repair of DNA damage of Jurkat cells after MMC induction. Abnormal expression of blm is correlated to the drug resistance of leukemia cells.

Influence of Ca and pH on the uptake and effects of Cd in Folsomia candida exposed to simplified soil solutions.

PubMed

Ardestani, Masoud M; Ortiz, Maria Diez; van Gestel, Cornelis A M

2013-08-01

The present study sought to quantify the components of a biotic ligand model (BLM) for the effects of Cd on Folsomia candida (Collembola). Assuming that soil porewater is the main route of exposure and to exclude the effects of soil particles on metal availability, animals were exposed for 7 d to different Cd concentrations between 0.1 mM and 100 mM in simplified soil solutions at different Ca concentrations (0.2 mM, 0.8 mM, 3.2 mM, and 12.8 mM) or at different pH (5.0, 6.0, and 7.0). Higher Ca concentrations decreased the toxicity of Cd (adult survival) in test solutions, whereas toxicity was slightly lower at pH 7 and 6 than at pH 5, suggesting a mitigating effect of Ca and to a lesser extent pH on Cd toxicity to F. candida. Internal Cd concentrations in the animals increased with increasing exposure level but were significantly reduced by increasing Ca concentrations and were not significantly affected by pH. By using Langmuir isotherms, binding constants for Cd, Ca, and protons and the fraction of binding sites occupied by Cd were calculated and used to predict effects of Cd on survival. Predicted toxicity showed a good agreement with measured responses when Ca and pH were used as separate factors or combined together. The present study shows indications of protective effects of Ca but less of protons on the toxicity and uptake of Cd in F. candida on exposure to simplified soil solutions, which can be described using the principles of a biotic ligand model. Copyright © 2013 SETAC.

No short-term cytogenetic consequences of Hungarian red mud catastrophe.

PubMed

Gundy, Sarolta; Farkas, Gyöngyi; Székely, Gábor; Kásler, Miklós

2013-01-01

Red mud is an industrial waste produced in the process of alumina extraction from bauxite with concentrated NaOH. When the red mud-containing reservoir collapsed in Ajka Alumina Plant Hungary in October 2010, the most serious immediate effects were caused by the high alkalinity (pH ≥ 13) of the flood. Many persons suffered burn-like damage to tissues and contact with caustic desiccated ultra-fine dust with traces of toxic metals also caused irritation of upper respiratory tract and eyes. This catastrophe was unique from the point of view of genotoxic effects as well. Therefore cytogenetic examinations were carried out on inhabitants, either with burns (17 persons) or on those inhaling desiccated caustic dust (42 persons). Chromosomal aberration (CA) analysis and bleomycin (BLM)-sensitivity assays, as possible markers of effects, were studied in peripheral blood lymphocytes of persons within 4-6 weeks following the catastrophe. Controls were matched for age, sex and smoking habits, and also places of residence with different constituents of air pollution either from rural (59 persons), or from urban environments (59 persons). Neither spontaneous rate of CAs (1.47% vs. 1.69%) nor BLM-induced in vitro chromosomal breakage (0.79 vs. 0.83 break/cell) showed elevated rates when cytogenetic biomarkers of genotoxicity were compared between controls and exposed persons. Time spent in cleaning did not affect cytogenetic changes either (R(2) = 0.04). BLM-induced mutagen sensitivity was similar in exposed and control persons (27.1% vs. 30.5%). It seems that the red mud exposure does not appear to pose an immediate genotoxic hazard on residents when measured with cytogenetic methods. We recommend, however, that those involved in clean-up activities should be followed closely not only for overall health, but also for further genotoxic risk assessment, because the long-term hazards of ultra-fine fugitive dust particles with alkalinity of residual NaOH in red mud are still unknown.

The risk for developing cancer in Israeli ATM, BLM, and FANCC heterozygous mutation carriers.

PubMed

Laitman, Yael; Boker-Keinan, Lital; Berkenstadt, Michal; Liphsitz, Irena; Weissglas-Volkov, Daphna; Ries-Levavi, Liat; Sarouk, Ifat; Pras, Elon; Friedman, Eitan

2016-03-01

Cancer risks in heterozygous mutation carriers of the ATM, BLM, and FANCC genes are controversial. To shed light on this issue, cancer rates were evaluated by cross referencing asymptomatic Israeli heterozygous mutation carriers in the ATM, BLM, and FANCC genes with cancer diagnoses registered at the Israeli National Cancer Registry (INCR). Comparison of observed to expected Standardized Incidence Rates (SIR) was performed. Overall, 474 individuals participated in the study: 378 females; 25 Arab and 31 Jewish ATM carriers, 152 BLM carriers, and 170 FANCC carriers (all Ashkenazim). Age range at genotyping was 19-53 years (mean + SD 30.6 + 5 years). In addition, 96 males were included; 5, 34, and 57 ATM, BLM, and FANCC mutation carriers, respectively. Over 5-16 years from genotyping (4721 person/years), 15 new cancers were diagnosed in mutation carriers: 5 breast, 4 cervical, 3 melanomas, and one each bone sarcoma, pancreatic, and colorectal cancer. No single cancer diagnosis was more prevalent then expected in all groups combined or per gene analyzed. Specifically breast cancer SIR was 0.02-0.77. We conclude that Israeli ATM, BLM, and FANCC heterozygous mutation carriers are not at an increased risk for developing cancer. Copyright © 2015 Elsevier Inc. All rights reserved.

Double heterozygotes among breast cancer patients analyzed for BRCA1, CHEK2, ATM, NBN/NBS1, and BLM germ-line mutations.

PubMed

Sokolenko, Anna P; Bogdanova, Natalia; Kluzniak, Wojciech; Preobrazhenskaya, Elena V; Kuligina, Ekatherina S; Iyevleva, Aglaya G; Aleksakhina, Svetlana N; Mitiushkina, Natalia V; Gorodnova, Tatiana V; Bessonov, Alexandr A; Togo, Alexandr V; Lubiński, Jan; Cybulski, Cezary; Jakubowska, Anna; Dörk, Thilo; Imyanitov, Evgeny N

2014-06-01

17 double heterozygous (DH) breast cancer (BC) patients were identified upon the analysis of 5,391 affected women for recurrent Slavic mutations in BRCA1, CHEK2, NBN/NBS1, ATM, and BLM genes. Double heterozygosity was found for BRCA1 and BLM (4 patients), BRCA1 and CHEK2 (4 patients), CHEK2 and NBS1 (3 patients), BRCA1 and ATM (2 patients), CHEK2 and BLM (2 patients), CHEK2 and ATM (1 patient), and NBS1 and BLM (1 patient). DH BC patients were on average not younger than single mutation carriers and did not have an excess of bilateral BC; an additional non-breast tumor was documented in two BRCA1/BLM DH patients (ovarian cancer and lymphoplasmacytic lymphoma). Loss-of-heterozygosity (LOH) analysis of involved genes was performed in 5 tumors, and revealed a single instance of somatic loss of the wild-type allele (LOH at CHEK2 locus in BRCA1/CHEK2 double heterozygote). Distribution of mutations in patients and controls favors the hypothesis on multiplicative interaction between at least some of the analyzed genes. Other studies on double heterozygosity for BC-predisposing germ-line mutations are reviewed.

Structural features facilitating tumor cell targeting and internalization by bleomycin and its disaccharide.

PubMed

Yu, Zhiqiang; Paul, Rakesh; Bhattacharya, Chandrabali; Bozeman, Trevor C; Rishel, Michael J; Hecht, Sidney M

2015-05-19

We have shown previously that the bleomycin (BLM) carbohydrate moiety can recapitulate the tumor cell targeting effects of the entire BLM molecule, that BLM itself is modular in nature consisting of a DNA-cleaving aglycone which is delivered selectively to the interior of tumor cells by its carbohydrate moiety, and that there are disaccharides structurally related to the BLM disaccharide which are more efficient than the natural disaccharide at tumor cell targeting/uptake. Because BLM sugars can deliver molecular cargoes selectively to tumor cells, and thus potentially form the basis for a novel antitumor strategy, it seemed important to consider additional structural features capable of affecting the efficiency of tumor cell recognition and delivery. These included the effects of sugar polyvalency and net charge (at physiological pH) on tumor cell recognition, internalization, and trafficking. Since these parameters have been shown to affect cell surface recognition, internalization, and distribution in other contexts, this study has sought to define the effects of these structural features on tumor cell recognition by bleomycin and its disaccharide. We demonstrate that both can have a significant effect on tumor cell binding/internalization, and present data which suggests that the metal ions normally bound by bleomycin following clinical administration may significantly contribute to the efficiency of tumor cell uptake, in addition to their characterized function in DNA cleavage. A BLM disaccharide-Cy5** conjugate incorporating the positively charged dipeptide d-Lys-d-Lys was found to associate with both the mitochondria and the nuclear envelope of DU145 cells, suggesting possible cellular targets for BLM disaccharide-cytotoxin conjugates.

Structural Features Facilitating Tumor Cell Targeting and Internalization by Bleomycin and Its Disaccharide

PubMed Central

2016-01-01

We have shown previously that the bleomycin (BLM) carbohydrate moiety can recapitulate the tumor cell targeting effects of the entire BLM molecule, that BLM itself is modular in nature consisting of a DNA-cleaving aglycone which is delivered selectively to the interior of tumor cells by its carbohydrate moiety, and that there are disaccharides structurally related to the BLM disaccharide which are more efficient than the natural disaccharide at tumor cell targeting/uptake. Because BLM sugars can deliver molecular cargoes selectively to tumor cells, and thus potentially form the basis for a novel antitumor strategy, it seemed important to consider additional structural features capable of affecting the efficiency of tumor cell recognition and delivery. These included the effects of sugar polyvalency and net charge (at physiological pH) on tumor cell recognition, internalization, and trafficking. Since these parameters have been shown to affect cell surface recognition, internalization, and distribution in other contexts, this study has sought to define the effects of these structural features on tumor cell recognition by bleomycin and its disaccharide. We demonstrate that both can have a significant effect on tumor cell binding/internalization, and present data which suggests that the metal ions normally bound by bleomycin following clinical administration may significantly contribute to the efficiency of tumor cell uptake, in addition to their characterized function in DNA cleavage. A BLM disaccharide-Cy5** conjugate incorporating the positively charged dipeptide d-Lys-d-Lys was found to associate with both the mitochondria and the nuclear envelope of DU145 cells, suggesting possible cellular targets for BLM disaccharide–cytotoxin conjugates. PMID:25905565

Biomembrane disruption by silica-core nanoparticles: effect of surface functional group measured using a tethered bilayer lipid membrane

PubMed Central

Liu, Ying; Zhang, Zhen; Zhang, Quanxuan; Baker, Gregory L.; Worden, R. Mark

2013-01-01

Engineered nanomaterials (ENM) have desirable properties that make them well suited for many commercial applications. However, a limited understanding of how ENM’s properties influence their molecular interactions with biomembranes hampers efforts to design ENM that are both safe and effective. This paper describes the use of a tethered bilayer lipid membrane (tBLM) to characterize biomembrane disruption by functionalized silica-core nanoparticles. Electrochemical impedance spectroscopy was used to measure the time trajectory of tBLM resistance following nanoparticle exposure. Statistical analysis of parameters from an exponential resistance decay model was then used to quantify and analyze differences between the impedance profiles of nanoparticles that were unfunctionalized, amine-functionalized, or carboxyl-functionalized. All of the nanoparticles triggered a decrease in membrane resistance, indicating nanoparticle-induced disruption of the tBLM. Hierarchical clustering allowed the potency of nanoparticles for reducing tBLM resistance to be ranked in the order amine > carboxyl ~ bare silica. Dynamic light scattering analysis revealed that tBLM exposure triggered minor coalescence for bare and amine-functionalized silica nanoparticles but not for carboxyl-functionalized silica nanoparticles. These results indicate that the tBLM method can reproducibly characterize ENM-induced biomembrane disruption and can distinguish the BLM-disruption patterns of nanoparticles that are identical except for their surface functional groups. The method provides insight into mechanisms of molecular interaction involving biomembranes and is suitable for miniaturization and automation for high-throughput applications to help assess the health risk of nanomaterial exposure or identify ENM having a desired mode of interaction with biomembranes. PMID:24060565

Basolateral membrane chloride permeability of A6 cells: implication in cell volume regulation.

PubMed

Brochiero, E; Banderali, U; Lindenthal, S; Raschi, C; Ehrenfeld, J

1995-11-01

The permeability to Cl- of the basolateral membrane (blm) was investigated in renal (A6) epithelial cells, assessing their role in transepithelial ion transport under steady-state conditions (isoosmotic) and following a hypoosmotic shock (i.e. in a regulatory volume decrease, RVD). Three different complementary studies were made by measuring: (1) the Cl- transport rates (delta F/Fo s-1 (x10(-3))), where F is the fluorescence of N-(6-methoxyquinoyl) acetoethyl ester, MQAE, and Fo the maximal fluorescence (x10(-3)) of both membranes by following the intracellular Cl- activities (ai Cl-, measured with MQAE) after extracellular Cl- substitution (2) the blm 86Rb and 36Cl uptakes and (3) the cellular potential and Cl- current using the whole-cell patch-clamp technique to differentiate between the different Cl- transport mechanisms. The permeability of the blm to Cl- was found to be much greater than that of the apical membranes under resting conditions: aiCl- changes were 5.3 +/- 0.7 mM and 25.5 +/- 1.05 mM (n = 79) when Cl- was substituted by NO3(-) in the media bathing apical and basolateral membranes. The Cl- transport rate of the blm was blocked by bumetanide (100 microM) and 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, 50 microM) but not by N-phenylanthranilic acid (DPC, 100 microM). 86Rb and 36Cl uptake experiments confirmed the presence of a bumetanide- and a NPPB-sensitive Cl- pathway, the latter being approximately three times more important than the former (Na/K/2Cl cotransporter). Appli-cation of a hypoosmotic medium to the serosal side of the cell increased delta F/Fo s-1 (x10(-3)) after extracellular Cl- substitution (1.03 +/- 0.10 and 2.45 +/- 0.17 arbitrary fluorescent units s-1 for isoosmotic and hypoosmotic conditions respectively, n = 11); this delta F/Fo s-1 (x10(-3)) increase was totally blocked by serosal NPPB application; on the other hand, cotransporter activity was decreased by the hypoosmotic shock. Cellular Ca2+ depletion had no effect on delta F/Fo s-1 (x10(-3)) under isoosmotic conditions, but blocked the delta F/Fo s-1 (x10(-3)) increase induced by a hypoosmotic stress. Under isotonic conditions the measured cellular potential at rest was -37.2 +/- 4.0 mV but reached a maximal and transient depolarization of -25.1 +/- 3.7 mV (n = 9) under hypoosmotic conditions. The cellular current at a patch-clamping cellular potential of -85 mV (close to the Nernst equilibrium potential for K+) was blocked by NPPB and transiently increased by hypoosmotic shock (≈50% maximum increase). This study demonstrates that the major component of Cl- transport through the blm of the A6 monolayer is a conductive pathway (NPPB-sensitive Cl- channels) and not a Na/K/2Cl cotransporter. These channels could play a role in transepithelial Cl- absorption and cell volume regulation. The increase in the blm Cl- conductance, inducing a depolarization of these membranes, is proposed as one of the early events responsible for the stimulation of the 86Rb efflux involved in cell volume regulation.

Evaluation and Development of Navigation Positioning and Monitoring Protocols for Dredged Material Disposal in Puget Sound.

DTIC Science & Technology

1986-02-01

Orlando. FL 3000 (305)281-S000 3S00( 6 ) eodmeter. Inc. Geodimeter 14-A 6000 t(S mm # 3 ppm) 511.300 Novato, CA 8000 (41S)677.12S6 15000 Ghodimeter 112...1.2 2.2 3.0 1 t(5mm + 5 ppm) t2" $19.950 Novato. CA 1.8 3.0 4.0 3 (415)883-2367 2.4 3.8 5.5 6 3.6 4.8 6.0 8 i-r Instrumnts EI/DM503 Automatic 1.5 2.5...POSITIONING LIMITATIONS FOR DREDGED MATERIAL DISPOSAL 4 BARGE MANEUVERABILITY 4 LIMITATIONS OF POSITIONING METHODS 5 Accuracy and Error 6 Site-Related

43 CFR 2806.30 - What are the rents for communication site rights-of-way?

Code of Federal Regulations, 2012 CFR

2012-10-01

... communication use rent schedule on the BLM Home Page on the Internet at http://www.blm.gov. (2) BLM will revise...: Communication Use Rent Schedule Annual Fees [Calendar year 2005] Population Television broadcast Am/FM radio...,000 1,489.63 1,117.22 744.81 124.14 744.81 3,103.39 434.47 1,862.03 93.10 1 Rent for AM Radio is 70...

43 CFR 2806.30 - What are the rents for communication site rights-of-way?

Code of Federal Regulations, 2013 CFR

2013-10-01

... communication use rent schedule on the BLM Home Page on the Internet at http://www.blm.gov. (2) BLM will revise...: Communication Use Rent Schedule Annual Fees [Calendar year 2005] Population Television broadcast Am/FM radio...,000 1,489.63 1,117.22 744.81 124.14 744.81 3,103.39 434.47 1,862.03 93.10 1 Rent for AM Radio is 70...

43 CFR 2806.30 - What are the rents for communication site rights-of-way?

Code of Federal Regulations, 2014 CFR

2014-10-01

... communication use rent schedule on the BLM Home Page on the Internet at http://www.blm.gov. (2) BLM will revise...: Communication Use Rent Schedule Annual Fees [Calendar year 2005] Population Television broadcast Am/FM radio...,000 1,489.63 1,117.22 744.81 124.14 744.81 3,103.39 434.47 1,862.03 93.10 1 Rent for AM Radio is 70...

Effects of CaO on the compaction and sintering by plasma of Powder-metallurgical iron

NASA Astrophysics Data System (ADS)

Ariza Suarez, H. G.; Sarmiento Santos, A.; Ortiz Otálora, C. A.

2017-12-01

This work the effect of the addition of Calcium Oxide (CaO) in the compaction and sintering of powder metallurgical iron Ancoor Steel 1000® is studied. Iron samples were made with proportions of: 0.5%, 1%, 1.5% and 2% by weight of CaO. The samples were sintered in a luminescent discharge furnace, in an atmosphere of H2+Ar at a temperature of 1150°C. XRD analysis was used to determine the formation of compounds, this analysis evidenced the formation of: hematite and magnetite, which were found both on the surface and in the volume. A characterization of the ability to protect against corrosive effects was carried out using the EIS electrochemical impedance spectroscopy method on the samples, in a solution of 1000ppm of chloride, with this procedure it was found that at a concentration of 0.5% and 1% CaO, the electrochemical impedance value is increased with values of 11.7MΩ, 2.2MΩ respectively.

Thermophysical Properties and Spectral Characterization of EA 6043

DTIC Science & Technology

2014-10-01

TGS) detector. The vapor-phase FTIR spectrum was measured using a Nicolet (Thermo Scientific Dionex ; Sunnyvale, CA) 6700 FTIR system equipped with a...3), 195 (1). A small feature near 267 m/z is probably associated with the parent ion . Figure 9. EI-MS of EA 6043. m/z 15 3.6 Nuclear...photometric detector with a phosphorus filter GC gas chromatography MCT mercury–cadmium telluride m/z mass-to-charge ratio NIR near infrared

Drill baby drill: An analysis of how energy development displaced ranching's dominance over the BLM's subgovernment policymaking environment

NASA Astrophysics Data System (ADS)

Forbis, Robert Earl, Jr.

Academic literature analyzing the Bureau of Land Management (BLM) land-use subgovernment stops at the Taylor Grazing Act and concludes that the historical development of administering grazing on public lands led to the capture of the BLM by ranching interests. Using a two-pronged methodological approach of process tracing and elite interviews this dissertation seeks to advance our collective knowledge of subgovernment theory by (a) clarifying the impact executive decision-making has on subgovernments and (b) identifying the conditions under which strategically competitive behavior between two competing subgovernment actors occurs. The dissertation seeks to update the literature by explaining what has caused the BLM to shift from a rancher-dominated agency to an energy dominated agency by identifying conditions under which subgovernment actors strategically respond to a political conflict. The research poses two questions: (1) how have executive actions disrupted an existing balance of power in a so-called "strong corner" of an entrenched subgovernment system and (2) what happens when conflict and competition break out between allied members of the system? Analysis indicates that as the BLM responded to Executive actions emphasizing domestic energy production, a conflict emerged between traditional allies: ranching and energy. Triggered by the unintended consequence of awakening long-dormant legislation, split-estate energy development---where property rights are severed between private surface and federal mineral estates---expanded across the West. In turn, this expansion helped establish the conditions for conflict and in doing so disrupted the balance of power between large public resource use interests in the relatively stable land-use subgovernment of the BLM. Indicative of energy's emerging dominance of the BLM's subgovernment, split-estate energy development led ranching interests to seek the protection of their Western state legislatures. This shift in domination led to a series of fiercely competitive political responses between the formerly allied interest groups. I argue that as political conflict intensified it is clear that the BLM's land-use policies are no longer dominated by ranching interests, but are now dominated by energy development interests. The analysis concludes that this shift in domination disproves the long accepted conclusion that the BLM is forever an agency captured by ranching interests.

Evaluation of acute copper toxicity to juvenile freshwater mussels (fatmucket, lampsilis siliquoidea) in natural and reconstituted waters

USGS Publications Warehouse

Wang, N.; Mebane, C.A.; Kunz, J.L.; Ingersoll, C.G.; May, T.W.; Arnold, W.R.; Santore, R.C.; Augspurger, T.; Dwyer, F.J.; Barniiart, M.C.

2009-01-01

The influence of dissolved organic carbon (DOC) and water composition on the toxicity of copper to juvenile freshwater mussels (fatmucket, Lampsilis siliquoidea) were evaluated in natural and reconstituted waters. Acute 96-h copper toxicity tests were conducted at four nominal DOC concentrations (0, 2.5, 5, and 10 mg/L as carbon [C]) in dilutions of natural waters and in American Society for Testing and Materials (ASTM) reconstituted hard water. Toxicity tests also were conducted in ASTM soft, moderately hard, hard, and very hard reconstituted waters (nominal hardness 45-300 mg/L as CaCO3). Three natural surface waters (9.5-11 mg/L DOC) were diluted to obtain a series of DOC concentrations with diluted well water, and an extract of natural organic matter and commercial humic acid was mixed with ASTM hard water to prepare a series of DOC concentrations for toxicity testing. Median effective concentrations (EC50s) for dissolved copper varied >40-fold (9.9 to >396 ??g Cu/L) over all 21 treatments in various DOC waters. Within a particular type of DOC water, EC50s increased 5- to 12-fold across DOC concentrations of 0.3 to up to 11 mg C/L. However, EC50s increased by only a factor of 1.4 (21 30 ??g Cu/L) in the four ASTM waters with wide range of water hardness (52-300 mg CaCO 3/L). Predictions from the biotic ligand model (BLM) for copper explained nearly 90% of the variability in EC50s. Nearly 70% of BLM-normalized EC50s for fatmucket tested in natural waters were below the final acute value used to derive the U.S. Environmental Protection Agency acute water quality criterion for copper, indicating that the criterion might not be protective of fatmucket and perhaps other mussel species. ?? 2009 SETAC.

Original Research: ACE2 activator associated with physical exercise potentiates the reduction of pulmonary fibrosis

PubMed Central

Prata, Luana O; Rodrigues, Carolina R; Martins, Jéssica M; Vasconcelos, Paula C; Oliveira, Fabrício Marcus S; Ferreira, Anderson J; Rodrigues-Machado, Maria da Glória

2016-01-01

The interstitial lung diseases are poorly understood and there are currently no studies evaluating the association of physical exercise with an ACE2 activator (DIZE) as a possible treatment for this group of diseases. We evaluate the effects of pharmacological treatment with an angiotensin-converting enzyme 2 activator drug, associated with exercise, on the pulmonary lesions induced by bleomycin. From the 96 male Balb/c mice used in the experiment, only 49 received 8 U/kg of bleomycin (BLM, intratracheally). The mice were divided into control (C) and bleomycin (BLM) groups, sedentary and trained (C-SED, C-EXE, BLM-SED, BLM-EXE), control and bleomycin and also sedentary and trained treated with diminazene (C-SED/E, C-EXE/E, BLM-SED/E, BLM-EXE/E). The animals were trained five days/week, 1 h/day with 60% of the maximum load obtained in a functional capacity test, for four weeks. Diminazene groups were treated (1 mg/kg, by gavage) daily until the end of the experiment. The lungs were collected 48 h after the training program, set in buffered formalin and investigated by Gomori’s trichrome, immunohistochemistry of collagen type I, TGF-β1, beta-prolyl-4-hydroxylase, MMP-1 and -2. The BLM-EXE/E group obtained a significant increase in functional capacity, reduced amount of fibrosis and type I collagen, decreased expression of TGF-β1 and beta-prolyl-4-hydroxylase and an increase of metalloproteinase −1, −2 when compared with the other groups. The present research shows, for the first time, that exercise training associated with the activation of ACE2 potentially reduces pulmonary fibrosis. PMID:27550926

Inhibition of bleomycin-induced pulmonary fibrosis by bone marrow-derived mesenchymal stem cells might be mediated by decreasing MMP9, TIMP-1, INF-γ and TGF-β.

PubMed

Yu, Shi-huan; Liu, Li-jie; Lv, Bin; Che, Chun-li; Fan, Da-ping; Wang, Li-feng; Zhang, Yi-mei

2015-08-01

The study was aimed to investigate the mechanism and administration timing of bone marrow-derived mesenchymal stem cells (BMSCs) in bleomycin (BLM)-induced pulmonary fibrosis mice. Thirty-six mice were divided into six groups: control group (saline), model group (intratracheal administration of BLM), day 1, day 3 and day 6 BMSCs treatment groups and hormone group (hydrocortisone after BLM treatment). BMSCs treatment groups received BMSCs at day 1, 3 or 6 following BLM treatment, respectively. Haematoxylin and eosin and Masson staining were conducted to measure lung injury and fibrosis, respectively. Matrix metalloproteinase (MMP9), tissue inhibitor of metalloproteinase-1 (TIMP-1), γ-interferon (INF-γ) and transforming growth factor β1 (TGF-β) were detected in both lung tissue and serum. Histologically, the model group had pronounced lung injury, increased inflammatory cells and collagenous fibres and up-regulated MMP9, TIMP-1, INF-γ and TGF-β compared with control group. The histological appearance of lung inflammation and fibrosis and elevation of these parameters were inhibited in BMSCs treatment groups, among which, day 3 and day 6 treatment groups had less inflammatory cells and collagenous fibres than day 1 treatment group. BMSCs might suppress lung fibrosis and inflammation through down-regulating MMP9, TIMP-1, INF-γ and TGF-β. Delayed BMSCs treatment might exhibit a better therapeutic effect. Highlights are as follows: 1. BMSCs repair lung injury induced by BLM. 2. BMSCs attenuate pulmonary fibrosis induced by BLM. 3. BMSCs transplantation down-regulates MMP9 and TIMP-1. 4. BMSCs transplantation down-regulates INF-γ and TGF-β. 5. Delayed transplantation timing of BMSCs might exhibit a better effect against BLM. Copyright © 2015 John Wiley & Sons, Ltd.

Bloom syndrome ortholog HIM-6 maintains genomic stability in C. elegans.

PubMed

Grabowski, Melissa M; Svrzikapa, Nenad; Tissenbaum, Heidi A

2005-12-01

Bloom syndrome is caused by mutation of the Bloom helicase (BLM), a member of the RecQ helicase family. Loss of BLM function results in genomic instability that causes a high incidence of cancer. It has been demonstrated that BLM is important for maintaining genomic stability by playing a role in DNA recombination and repair; however, the exact function of BLM is not clearly understood. To determine the mechanism by which BLM controls genomic stability in vivo, we examined the phenotypes caused by mutation of the C. elegans BLM helicase ortholog, HIM-6. We find that the loss of HIM-6 leads to genomic instability as evidenced by an increased number of genomic insertions and deletions, which results in visible random mutant phenotypes. In addition to the mutator phenotype, him-6 mutants have a low brood size, a high incidence of males, a shortened life span, and an increased amount of germ line apoptosis. Upon exposure to high temperature, him-6 mutants that are serially passed become sterile demonstrating a mortal germ line phenotype. Our data suggest a model in which loss of HIM-6 results in genomic instability due to an increased number of DNA lesions, which either cannot be repaired and/or are introduced by low fidelity recombination events. The increased level of genomic instability that leads to him-6(ok412) mutants having a shortened life span.

Regulation of gene expression by the BLM helicase correlates with the presence of G-quadruplex DNA motifs

PubMed Central

Nguyen, Giang Huong; Tang, Weiliang; Robles, Ana I.; Beyer, Richard P.; Gray, Lucas T.; Welsh, Judith A.; Schetter, Aaron J.; Kumamoto, Kensuke; Wang, Xin Wei; Hickson, Ian D.; Maizels, Nancy; Monnat, Raymond J.; Harris, Curtis C.

2014-01-01

Bloom syndrome is a rare autosomal recessive disorder characterized by genetic instability and cancer predisposition, and caused by mutations in the gene encoding the Bloom syndrome, RecQ helicase-like (BLM) protein. To determine whether altered gene expression might be responsible for pathological features of Bloom syndrome, we analyzed mRNA and microRNA (miRNA) expression in fibroblasts from individuals with Bloom syndrome and in BLM-depleted control fibroblasts. We identified mRNA and miRNA expression differences in Bloom syndrome patient and BLM-depleted cells. Differentially expressed mRNAs are connected with cell proliferation, survival, and molecular mechanisms of cancer, and differentially expressed miRNAs target genes involved in cancer and in immune function. These and additional altered functions or pathways may contribute to the proportional dwarfism, elevated cancer risk, immune dysfunction, and other features observed in Bloom syndrome individuals. BLM binds to G-quadruplex (G4) DNA, and G4 motifs were enriched at transcription start sites (TSS) and especially within first introns (false discovery rate ≤ 0.001) of differentially expressed mRNAs in Bloom syndrome compared with normal cells, suggesting that G-quadruplex structures formed at these motifs are physiologic targets for BLM. These results identify a network of mRNAs and miRNAs that may drive the pathogenesis of Bloom syndrome. PMID:24958861

Regulation of gene expression by the BLM helicase correlates with the presence of G-quadruplex DNA motifs.

PubMed

Nguyen, Giang Huong; Tang, Weiliang; Robles, Ana I; Beyer, Richard P; Gray, Lucas T; Welsh, Judith A; Schetter, Aaron J; Kumamoto, Kensuke; Wang, Xin Wei; Hickson, Ian D; Maizels, Nancy; Monnat, Raymond J; Harris, Curtis C

2014-07-08

Bloom syndrome is a rare autosomal recessive disorder characterized by genetic instability and cancer predisposition, and caused by mutations in the gene encoding the Bloom syndrome, RecQ helicase-like (BLM) protein. To determine whether altered gene expression might be responsible for pathological features of Bloom syndrome, we analyzed mRNA and microRNA (miRNA) expression in fibroblasts from individuals with Bloom syndrome and in BLM-depleted control fibroblasts. We identified mRNA and miRNA expression differences in Bloom syndrome patient and BLM-depleted cells. Differentially expressed mRNAs are connected with cell proliferation, survival, and molecular mechanisms of cancer, and differentially expressed miRNAs target genes involved in cancer and in immune function. These and additional altered functions or pathways may contribute to the proportional dwarfism, elevated cancer risk, immune dysfunction, and other features observed in Bloom syndrome individuals. BLM binds to G-quadruplex (G4) DNA, and G4 motifs were enriched at transcription start sites (TSS) and especially within first introns (false discovery rate ≤ 0.001) of differentially expressed mRNAs in Bloom syndrome compared with normal cells, suggesting that G-quadruplex structures formed at these motifs are physiologic targets for BLM. These results identify a network of mRNAs and miRNAs that may drive the pathogenesis of Bloom syndrome.

Framework for Derivation of Water Quality Criteria Using the Biotic Ligand Model: Copper as a Case Study.

PubMed

Gondek, John C; Gensemer, Robert W; Claytor, Carrie A; Canton, Steven P; Gorsuch, Joseph W

2018-06-01

Acceptance of the Biotic Ligand Model (BLM) to derive aquatic life criteria, for metals in general and copper in particular, is growing amongst regulatory agencies worldwide. Thus, it is important to ensure that water quality data are used appropriately and consistently in deriving such criteria. Here we present a suggested BLM implementation framework (hereafter referred to as "the Framework") to help guide the decision-making process when designing sampling and analysis programs for use of the BLM to derive water quality criteria applied on a site-specific basis. Such a framework will help inform stakeholders on the requirements needed to derive BLM-based criteria, and thus, ensure the appropriate types and amount of data are being collected and interpreted. The Framework was developed for calculating BLM-based criteria when data are available from multiple sampling locations on a stream. The Framework aspires to promote consistency when applying the BLM across datasets of disparate water quality, data quantity, and spatial and temporal representativeness, and is meant to be flexible to maximize applicability over a wide range of scenarios. Therefore, the Framework allows for a certain level of interpretation and adjustment to address the issues unique to each dataset. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Bent Bragg–Laue monochromator for high-energy X-rays

DOE PAGES

Shi, Xianbo; Xu, Wenqian; Yakovenko, Andrey; ...

2017-07-26

A bent Bragg–Laue monochromator (BLM) is proposed for high-energy X-ray (~25–60 keV) beamlines. The BLM has the unique feature of bi-directional focusing. A sagittally bent Laue crystal can focus the large horizontal fan of a bending magnet or wiggler source. A meridionally bent Bragg crystal focuses the beam vertically and corrects for the anticlastic bending effects of the Laue crystal. This monochromator geometry relies on the crystal orientations being optimized. We show that the focusing condition and Rowland condition can be simultaneously satisfied at a given energy. A detailed ray tracings indicate that a BLM can provide similar energy resolutionmore » and higher flux density compared to a sagittally bent double-Laue monochromator configuration. A prototype BLM with a symmetric Bragg crystal and an asymmetric Laue crystal was tested. Matching of the bend radii of the two crystals in the meridional direction was demonstrated. Generally, the horizontal acceptance of the sagittally bent Laue crystal is limited by the large curvature. This horizontal BLM acceptance could be increased by translating the Laue crystal along its sagittal bending axis.« less

Bent Bragg–Laue monochromator for high-energy X-rays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shi, Xianbo; Xu, Wenqian; Yakovenko, Andrey

A bent Bragg–Laue monochromator (BLM) is proposed for high-energy X-ray (~25–60 keV) beamlines. The BLM has the unique feature of bi-directional focusing. A sagittally bent Laue crystal can focus the large horizontal fan of a bending magnet or wiggler source. A meridionally bent Bragg crystal focuses the beam vertically and corrects for the anticlastic bending effects of the Laue crystal. This monochromator geometry relies on the crystal orientations being optimized. We show that the focusing condition and Rowland condition can be simultaneously satisfied at a given energy. A detailed ray tracings indicate that a BLM can provide similar energy resolutionmore » and higher flux density compared to a sagittally bent double-Laue monochromator configuration. A prototype BLM with a symmetric Bragg crystal and an asymmetric Laue crystal was tested. Matching of the bend radii of the two crystals in the meridional direction was demonstrated. Generally, the horizontal acceptance of the sagittally bent Laue crystal is limited by the large curvature. This horizontal BLM acceptance could be increased by translating the Laue crystal along its sagittal bending axis.« less

Structural and Electrochemical Characteristics of Ca-Doped “Flower-like” Li 4Ti 5O 12 Motifs as High-Rate Anode Materials for Lithium-Ion Batteries

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Lei; Zhang, Yiman; Guo, Haoyue

Doped motifs offer an intriguing structural pathway toward improving conductivity for battery applications. Specifically, Ca-doped, three-dimensional “flower-like” Li 4–xCa xTi 5O 12 (“x” = 0, 0.1, 0.15, and 0.2) micrometer-scale spheres have been successfully prepared for the first time using a simple and reproducible hydrothermal reaction followed by a short calcination process. The products were experimentally characterized by means of X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) mapping, inductively coupled plasma optical emission spectrometry (ICP-OES), X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and galvanostatic charge–discharge testing. Calcium dopantmore » ions were shown to be uniformly distributed within the LTO structure without altering the underlying “flower-like” morphology. The largest lattice expansion and the highest Ti 3+ ratios were noted with XRD and XPS, respectively, whereas increased charge transfer conductivity and decreased Li +-ion diffusion coefficients were displayed in EIS for the Li 4–xCa xTi 5O 12 (“x” = 0.2) sample. The “x” = 0.2 sample yielded a higher rate capability, an excellent reversibility, and a superior cycling stability, delivering 151 and 143 mAh/g under discharge rates of 20C and 40C at cycles 60 and 70, respectively. In addition, a high cycling stability was demonstrated with a capacity retention of 92% after 300 cycles at a very high discharge rate of 20C. In addition, first-principles calculations based on density functional theory (DFT) were conducted with the goal of further elucidating and understanding the nature of the doping mechanism in this study. The DFT calculations not only determined the structure of the Ca-doped Li 4Ti 5O 12, which was found to be in accordance with the experimentally measured XPD pattern, but also yielded valuable insights into the doping-induced effect on both the atomic and electronic structures of Li 4Ti 5O 12.« less

Structural and Electrochemical Characteristics of Ca-Doped “Flower-like” Li 4Ti 5O 12 Motifs as High-Rate Anode Materials for Lithium-Ion Batteries

DOE PAGES

Wang, Lei; Zhang, Yiman; Guo, Haoyue; ...

2018-01-22

Doped motifs offer an intriguing structural pathway toward improving conductivity for battery applications. Specifically, Ca-doped, three-dimensional “flower-like” Li 4–xCa xTi 5O 12 (“x” = 0, 0.1, 0.15, and 0.2) micrometer-scale spheres have been successfully prepared for the first time using a simple and reproducible hydrothermal reaction followed by a short calcination process. The products were experimentally characterized by means of X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) mapping, inductively coupled plasma optical emission spectrometry (ICP-OES), X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and galvanostatic charge–discharge testing. Calcium dopantmore » ions were shown to be uniformly distributed within the LTO structure without altering the underlying “flower-like” morphology. The largest lattice expansion and the highest Ti 3+ ratios were noted with XRD and XPS, respectively, whereas increased charge transfer conductivity and decreased Li +-ion diffusion coefficients were displayed in EIS for the Li 4–xCa xTi 5O 12 (“x” = 0.2) sample. The “x” = 0.2 sample yielded a higher rate capability, an excellent reversibility, and a superior cycling stability, delivering 151 and 143 mAh/g under discharge rates of 20C and 40C at cycles 60 and 70, respectively. In addition, a high cycling stability was demonstrated with a capacity retention of 92% after 300 cycles at a very high discharge rate of 20C. In addition, first-principles calculations based on density functional theory (DFT) were conducted with the goal of further elucidating and understanding the nature of the doping mechanism in this study. The DFT calculations not only determined the structure of the Ca-doped Li 4Ti 5O 12, which was found to be in accordance with the experimentally measured XPD pattern, but also yielded valuable insights into the doping-induced effect on both the atomic and electronic structures of Li 4Ti 5O 12.« less

Influence of dissolved organic carbon on toxicity of copper to a unionid mussel (Villosa iris) and a cladoceran (Ceriodaphnia dubia) in acute and chronic water exposures

USGS Publications Warehouse

Wang, Ning; Mebane, Christopher A.; Kunz, James L.; Ingersoll, Christopher G.; Brumbaugh, William G.; Santore, Robert C.; Gorsuch, Joseph W.; Arnold, W. Ray

2011-01-01

Acute and chronic toxicity of copper (Cu) to a unionid mussel (Villosa iris) and a cladoceran (Ceriodaphnia dubia) were determined in water exposures at four concentrations of dissolved organic carbon (DOC; nominally 0.5, 2.5, 5, and 10 mg/L as carbon [C]). Test waters with DOC concentrations of 2.5 to 10 mg C/L were prepared by mixing a concentrate of natural organic matter (Suwannee River, GA, USA) in diluted well water (hardness 100 mg/L as CaCO3, pH 8.3, DOC 0.5 mg C/L). Acute median effect concentrations (EC50s) for dissolved Cu increased approximately fivefold (15–72 μg Cu/L) for mussel survival in 4-d exposures and increased about 11-fold (25–267 μg Cu/L) for cladoceran survival in 2-d exposures across DOC concentrations from 0.5 to 10 mg C/L. Similarly, chronic 20% effect concentrations (EC20s) for the mussel in 28-d exposures increased about fivefold (13–61 μg Cu/L for survival; 8.8–38 μg Cu/L for biomass), and the EC20s for the cladoceran in 7-d exposures increased approximately 17-fold (13–215 μg Cu/L) for survival or approximately fourfold (12–42 μg Cu/L) for reproduction across DOC concentrations from 0.5 to 10 mg C/L. The acute and chronic values for the mussel were less than or approximately equal to the values for the cladoceran. Predictions from the biotic ligand model (BLM) used to derive the U.S. Environmental Protection Agency's ambient water quality criteria (AWQC) for Cu explained more than 90% of the variation in the acute and chronic endpoints for the two species, with the exception of the EC20 for cladoceran reproduction (only 46% of variation explained). The BLM-normalized acute EC50s and chronic EC20s for the mussel and BLM-normalized chronic EC20s for the cladoceran in waters with DOC concentrations of 2.5 to 10 mg C/L were equal to or less than the final acute value and final chronic value in the BLM-based AWQC for Cu, respectively, indicating that the Cu AWQC might not adequately protect the mussel from acute and chronic exposure, and the cladoceran from chronic exposure.

Setting the renormalization scale in pQCD: Comparisons of the principle of maximum conformality with the sequential extended Brodsky-Lepage-Mackenzie approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ma, Hong -Hao; Wu, Xing -Gang; Ma, Yang

A key problem in making precise perturbative QCD (pQCD) predictions is how to set the renormalization scale of the running coupling unambiguously at each finite order. The elimination of the uncertainty in setting the renormalization scale in pQCD will greatly increase the precision of collider tests of the Standard Model and the sensitivity to new phenomena. Renormalization group invariance requires that predictions for observables must also be independent on the choice of the renormalization scheme. The well-known Brodsky-Lepage-Mackenzie (BLM) approach cannot be easily extended beyond next-to-next-to-leading order of pQCD. Several suggestions have been proposed to extend the BLM approach tomore » all orders. In this paper we discuss two distinct methods. One is based on the “Principle of Maximum Conformality” (PMC), which provides a systematic all-orders method to eliminate the scale and scheme ambiguities of pQCD. The PMC extends the BLM procedure to all orders using renormalization group methods; as an outcome, it significantly improves the pQCD convergence by eliminating renormalon divergences. An alternative method is the “sequential extended BLM” (seBLM) approach, which has been primarily designed to improve the convergence of pQCD series. The seBLM, as originally proposed, introduces auxiliary fields and follows the pattern of the β0-expansion to fix the renormalization scale. However, the seBLM requires a recomputation of pQCD amplitudes including the auxiliary fields; due to the limited availability of calculations using these auxiliary fields, the seBLM has only been applied to a few processes at low orders. In order to avoid the complications of adding extra fields, we propose a modified version of seBLM which allows us to apply this method to higher orders. As a result, we then perform detailed numerical comparisons of the two alternative scale-setting approaches by investigating their predictions for the annihilation cross section ratio R e+e– at four-loop order in pQCD.« less

Data resources for range-wide assessment of livestock grazing across the sagebrush biome

USGS Publications Warehouse

Assal, T.J.; Veblen, K.E.; Farinha, M.A.; Aldridge, Cameron L.; Casazza, Michael L.; Pyke, D.A.

2012-01-01

The data contained in this series were compiled, modified, and analyzed for the U.S. Geological Survey (USGS) report "Range-Wide Assessment of Livestock Grazing Across the Sagebrush Biome." This report can be accessed through the USGS Publications Warehouse (online linkage: http://pubs.usgs.gov/of/2011/1263/). The dataset contains spatial and tabular data related to Bureau of Land Management (BLM) Grazing Allotments. We reviewed the BLM national grazing allotment spatial dataset available from the GeoCommunicator National Integrated Land System (NILS) website in 2007 (http://www.geocommunicator.gov). We identified several limitations in those data and learned that some BLM State and/or field offices had updated their spatial data to rectify these limitations, but maintained the data outside of NILS. We contacted appropriate BLM offices (State or field, 25 in all) to obtain the most recent data, assessed the data, established a data development protocol, and compiled data into a topologically enforced dataset throughout the area of interest for this project (that is, the pre-settlement distribution of Greater Sage-Grouse in the Western United States). The final database includes three spatial datasets: Allotments (BLM Grazing Allotments), OUT_Polygons (nonallotment polygons used to ensure topology), and Duplicate_Polygon_Allotments. See Appendix 1 of the aforementioned report for complete methods. The tabular data presented here consists of information synthesized by the Land Health Standard (LHS) analysis (Appendix 2), and data obtained from the BLM Rangeland Administration System (http://www.blm.gov/ras/). In 2008, available LHS data for all allotments in all regions were compiled by BLM in response to a Freedom of Information Act (FOIA) request made by a private organization. The BLM provided us with a copy of these data. These data provided three major types of information that were of interest: (1) date(s) (if any) of the most recent LHS evaluation for each allotment; (2) whether if evaluated, each region-specific standard (3–8 LHS depending on region) had been met on a given allotment; and (3) whether livestock contributed to any of these standards not being met. A description of how we processed the original data to prepare for analysis is described in Appendix 2, and the synthesized dataset can be found in the table "lhs_x_walk." Permitted use dates, livestock type (horse, sheep or cattle), number of livestock, and Animal Unit Months [the number of animal units (1,000-pound animal equivalents) that can be grazed for 31 days with the available forage in a sustainable manner] are the legal maximum grazing amounts for a given allotment, and legal adjustments to these numbers occur infrequently. We summarized permitted use by BLM allotment in the table "Permitted_Use." Billed use records are used for calculations of permittees' annual grazing bills. We summarized billed use by allotment for BLM grazing year in the table "Billed_Use." All three tables can be joined with the allotment spatial data in a geographic information system (GIS) environment, using the IDENT attribute as the primary key.

Conversion of radioactive ferrocyanide compounds to immobile glasses

DOEpatents

Schulz, W.W.; Dressen, A.L.

1975-11-21

A method is described for converting complex radioactive ferrocyanide compounds of /sup 134/Cs and /sup 137/Cs to immobile glass that is resistant to leaching by water. The /sup 134/Cs and /sup 137/Cs are separated from nuclear waste solutions by precipitation from alkaline solutions by the addition of a soluble Ni/sup 2 +/, Zn/sup 2 +/, Cu/sup 2 +/, Co/sup 2 +/, UO/sub 2//sup 2 +/, or Mn/sup 2 +/ and K/sub 4/Fe(CN)/sub 6/. The dried, finely ground precipitate is mixed with Na/sub 2/CO/sub 3/ and a mixture of (a) basalt and B/sub 2/O/sub 3/ or (b) SiO/sub 2/ and CaO, melted, and allowed to solidify. (BLM)

Electrochemical and Morphological Investigations of Ga Addition to Pt Electrocatalyst Supported on Carbon

PubMed Central

Paganoto, Giordano T.; Santos, Deise M.; Guimarães, Marco C. C.; Carneiro, Maria Tereza W. D.

2017-01-01

This paper is consisted in the synthesis of platinum-based electrocatalysts supported on carbon (Vulcan XC-72) and investigation of the addition of gallium in their physicochemical and electrochemical properties toward ethanol oxidation reaction (EOR). PtGa/C electrocatalysts were prepared through thermal decomposition of polymeric precursor method at a temperature of 350°C. Six different compositions were homemade: Pt50Ga50/C, Pt60Ga40/C, Pt70Ga30/C, Pt80Ga20/C, Pt90Ga10/C, and Pt100/C. These electrocatalysts were electrochemically characterized by cyclic voltammetry (CV), chronoamperometry (CA), chronopotentiometry (CP), and electrochemical impedance spectroscopy (EIS) in the presence and absence of ethanol 1.0 mol L−1. Thermogravimetric analysis (TGA), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and transmission electron microscopy (TEM) were also carried out for a physicochemical characterization of those materials. XRD results showed the main peaks of face-centered cubic Pt. The particle sizes obtained from XRD and TEM analysis range from 7.2 nm to 12.9 nm. The CV results indicate behavior typical of Pt-based electrocatalysts in acid medium. The CV, EIS, and CA data reveal that the addition of up to 31% of gallium to the Pt highly improves catalytic activity on EOR response when compared to Pt100/C. PMID:28466065

Transportation and the Bureau of Land Management

DOT National Transportation Integrated Search

2008-01-01

The Volpe Center prepared a promotional booklet featuring an overview of the BLM's transportation infrastructure. The booklet describes the BLM's roads, bridges, and trails; highlights their economic and recreational importance; and lists key transpo...

Nonlinear Aeroelastic Study for Folding Wing Structures

DTIC Science & Technology

2010-03-29

Blm +B nl m ) ζ (14) δǫm = ( Blm +B nl m ) δζ (15) χ = Bbζ (16) δχ = Bbδζ . (17) Substitution of Eqs. 13-17 into Eq. 11 gives: δζT ( h...S ΨTFdS + ∫ S ΨTΦtrdS − h ∫ S ΨTΨρdSζ̈ − h ∫ S ΨT cΨdSζ̇ − ∫ S ( Blm ) TNdS − ∫ S (Bnlm) TNdS − ∫ S BTb MdS ) = 0 . (18) Considering the arbitrariness...of the virtual generalized displacements, Eq. 18 can be written 6 of 24 as: h ∫ S ΨTΨρdSζ̈ + h ∫ S ΨT cΨdSζ̇ + ∫ S ( Blm ) TNdS + ∫ S (Bnlm) TNdS+ ∫

Nonlinear High Fidelity Aeroelastic Analysis for Novel Configurations

DTIC Science & Technology

2010-03-29

Blm +B nl m ) ζ (14) δǫm = ( Blm +B nl m ) δζ (15) χ = Bbζ (16) δχ = Bbδζ . (17) Substitution of Eqs. 13-17 into Eq. 11 gives: δζT ( h...S ΨTFdS + ∫ S ΨTΦtrdS − h ∫ S ΨTΨρdSζ̈ − h ∫ S ΨT cΨdSζ̇ − ∫ S ( Blm ) TNdS − ∫ S (Bnlm) TNdS − ∫ S BTb MdS ) = 0 . (18) Considering the arbitrariness...of the virtual generalized displacements, Eq. 18 can be written 6 of 24 as: h ∫ S ΨTΨρdSζ̈ + h ∫ S ΨT cΨdSζ̇ + ∫ S ( Blm ) TNdS + ∫ S (Bnlm) TNdS+ ∫

Gas desorption and adsorption isotherm studies of coals in the Powder River basin, Wyoming and adjacent basins in Wyoming and North Dakota

USGS Publications Warehouse

Stricker, Gary D.; Flores, Romeo M.; McGarry, Dwain E.; Stillwell, Dean P.; Hoppe, Daniel J.; Stillwell, Cathy R.; Ochs, Alan M.; Ellis, Margaret S.; Osvald, Karl S.; Taylor, Sharon L.; Thorvaldson, Marjorie C.; Trippi, Michael H.; Grose, Sherry D.; Crockett, Fred J.; Shariff, Asghar J.

2006-01-01

The U.S. Geological Survey (USGS), in cooperation with the State Office, Reservoir Management Group (RMG), of the Bureau of Land Management (BLM) in Casper (Wyoming), investigated the coalbed methane resources (CBM) in the Powder River Basin, Wyoming and Montana, from 1999 to the present. Beginning in late 1999, the study also included the Williston Basin in Montana and North and South Dakota and Green River Basin and Big Horn Basin in Wyoming. The rapid development of CBM (referred to as coalbed natural gas by the BLM) during the early 1990s, and the lack of sufficient data for the BLM to fully assess and manage the resource in the Powder River Basin, in particular, gave impetus to the cooperative program. An integral part of the joint USGS-BLM project was the participation of 25 gas operators that entered individually into confidential agreements with the USGS, and whose cooperation was essential to the study. The arrangements were for the gas operators to drill and core coal-bed reservoirs at their cost, and for the USGS and BLM personnel to then desorb, analyze, and interpret the coal data with joint funding by the two agencies. Upon completion of analyses by the USGS, the data were to be shared with both the BLM and the gas operator that supplied the core, and then to be released or published 1 yr after the report was submitted to the operator.

Stage-specific apoptosis, developmental delay, and embryonic lethality in mice homozygous for a targeted disruption in the murine Bloom's syndrome gene.

PubMed

Chester, N; Kuo, F; Kozak, C; O'Hara, C D; Leder, P

1998-11-01

Bloom's syndrome is a human autosomal genetic disorder characterized at the cellular level by genome instability and increased sister chomatid exchanges (SCEs). Clinical features of the disease include proportional dwarfism and a predisposition to develop a wide variety of malignancies. The human BLM gene has been cloned recently and encodes a DNA helicase. Mouse embryos homozygous for a targeted mutation in the murine Bloom's syndrome gene (Blm) are developmentally delayed and die by embryonic day 13.5. The fact that the interrupted gene is the homolog of the human BLM gene was confirmed by its homologous sequence, its chromosomal location, and by demonstrating high numbers of SCEs in cultured murine Blm-/- fibroblasts. The proportional dwarfism seen in the human is consistent with the small size and developmental delay (12-24 hr) seen during mid-gestation in murine Blm-/- embryos. Interestingly, the growth retardation in mutant embryos can be accounted for by a wave of increased apoptosis in the epiblast restricted to early post-implantation embryogenesis. Mutant embryos do not survive past day 13.5, and at this time exhibit severe anemia. Red blood cells and their precursors from Blm-/- embryos are heterogeneous in appearance and have increased numbers of macrocytes and micronuclei. Both the apoptotic wave and the appearance of micronuclei in red blood cells are likely cellular consequences of damaged DNA caused by effects on replicating or segregating chromosomes.

The C-terminal domain of the Bloom syndrome DNA helicase is essential for genomic stability

PubMed Central

Yankiwski, Victor; Noonan, James P; Neff, Norma F

2001-01-01

Background Bloom syndrome is a rare cancer-prone disorder in which the cells of affected persons have a high frequency of somatic mutation and genomic instability. Bloom syndrome cells have a distinctive high frequency of sister chromatid exchange and quadriradial formation. BLM, the protein altered in BS, is a member of the RecQ DNA helicase family, whose members share an average of 40% identity in the helicase domain and have divergent N-terminal and C-terminal flanking regions of variable lengths. The BLM DNA helicase has been shown to localize to the ND10 (nuclear domain 10) or PML (promyelocytic leukemia) nuclear bodies, where it associates with TOPIIIα, and to the nucleolus. Results This report demonstrates that the N-terminal domain of BLM is responsible for localization of the protein to the nuclear bodies, while the C-terminal domain directs the protein to the nucleolus. Deletions of the N-terminal domain of BLM have little effect on sister chromatid exchange frequency and chromosome stability as compared to helicase and C-terminal mutations which can increase SCE frequency and chromosome abnormalities. Conclusion The helicase activity and the C-terminal domain of BLM are critical for maintaining genomic stability as measured by the sister chromatid exchange assay. The localization of BLM into the nucleolus by the C-terminal domain appears to be more important to genomic stability than localization in the nuclear bodies. PMID:11472631

The FANC pathway and BLM collaborate during mitosis to prevent micro-nucleation and chromosome abnormalities.

PubMed

Naim, Valeria; Rosselli, Filippo

2009-06-01

Loss-of-function of caretaker genes characterizes a group of cancer predisposition diseases that feature cellular hypersensitivity to DNA damage and chromosome fragility; this group includes Fanconi anaemia and Bloom syndrome. The products of the 13 FANC genes (mutated in Fanconi anaemia), which constitute the 'FANC' pathway, and BLM (the RecQ helicase mutated in Bloom syndrome) are thought to collaborate during the S phase of the cell cycle, preventing chromosome instability. Recently, BLM has been implicated in the completion of sister chromatid separation during mitosis, a complex process in which precise regulation and execution is crucial to preserve genomic stability. Here we show for the first time a role for the FANC pathway in chromosome segregation during mitotic cell division. FANCD2, a key component of the pathway, localizes to discrete spots on mitotic chromosomes. FANCD2 chromosomal localization is responsive to replicative stress and specifically targets aphidicolin (APH)-induced chromatid gaps and breaks. Our data indicate that the FANC pathway is involved in rescuing abnormal anaphase and telophase (ana-telophase) cells, limiting aneuploidy and reducing chromosome instability in daughter cells. We further address a cooperative role for the FANC pathway and BLM in preventing micronucleation, through FANC-dependent targeting of BLM to non-centromeric abnormal structures induced by replicative stress. We reveal new crosstalk between FANC and BLM proteins, extending their interaction beyond the S-phase rescue of damaged DNA to the safeguarding of chromosome stability during mitosis.

Dynamics of bleomycin interaction with a strongly bound hairpin DNA substrate, and implications for cleavage of the bound DNA.

PubMed

Bozeman, Trevor C; Nanjunda, Rupesh; Tang, Chenhong; Liu, Yang; Segerman, Zachary J; Zaleski, Paul A; Wilson, W David; Hecht, Sidney M

2012-10-31

Recent studies involving DNAs bound strongly by bleomycins have documented that such DNAs are degraded by the antitumor antibiotic with characteristics different from those observed when studying the cleavage of randomly chosen DNAs in the presence of excess Fe·BLM. In the present study, surface plasmon resonance has been used to characterize the dynamics of BLM B(2) binding to a strongly bound hairpin DNA, to define the effects of Fe(3+), salt, and temperature on BLM-DNA interaction. One strong primary DNA binding site, and at least one much weaker site, were documented. In contrast, more than one strong cleavage site was found, an observation also made for two other hairpin DNAs. Evidence is presented for BLM equilibration between the stronger and weaker binding sites in a way that renders BLM unavailable to other, less strongly bound DNAs. Thus, enhanced binding to a given site does not necessarily result in increased DNA degradation at that site; i.e., for strongly bound DNAs, the facility of DNA cleavage must involve other parameters in addition to the intrinsic rate of C-4' H atom abstraction from DNA sugars.

[Study on the hepatocytic cell targetability of liposomes].

PubMed

Hou, Xin-pu; Wang, Li; Wang, Xiang-tao; Li, Sha

2003-02-01

To target for hepatocytic cell, liposomes was modified by special ligand. Sterically stabilized liposomes (SSL) was conjugated with asialofeticin (AF), the ligand of asialoglycoprotein receptor (ASGP-R) of hepatocyte. ASGP-R-BLM is the ASGP-R reconstructed on bilayer lipid membrane (BLM). The recognition reaction between AF-SSL and ASGP-R-BLM can be monitored by the varieties of membrane electrical parameters. The targetability of AF-SSL mediated to hepatocyte was detected by radioisotopic labeled in vitro and in vivo. The therapeutic effect of antihepatocarcinoma was observed also. The lifetime of ASGP-R-BLM decreased with the added amount of AF-SSL. It was demonstrated that there was recognition reaction between AF-SSL and ASGP-R-BLM. The combination of AF-SSL with hepatocyte was significantly higher than that of SSL without AF-modified in vitro and in vivo. The survival time of rat for AF-SSL carriered ADM (adriamycin) group was much longer and the toxicities on heart, kidney and lung were lower than those SSL carried ADM group. It is possible to actively target the cell with specific receptor by ligand modified liposomes. The result prvide scientific basis of hepatocyte targeted liposomes.

75 FR 3914 - Notice of Proposed Information Collection for 1004-0019

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-25

... handling and caring for domestic livestock authorized by the BLM to graze on public lands. This information... for the management and care of permitted livestock on grazing allotments. The BLM implements this...

43 CFR 3262.13 - May BLM require me to follow a well spacing program?

Code of Federal Regulations, 2011 CFR

2011-10-01

... RESOURCE LEASING Conducting Drilling Operations § 3262.13 May BLM require me to follow a well spacing... spacing: (a) Hydrologic, geologic, and reservoir characteristics of the field, minimizing well...

Inorganic/organic doped carbon aerogels as biosensing materials for the detection of hydrogen peroxide.

PubMed

Dong, Sheying; Li, Nan; Suo, Gaochao; Huang, Tinglin

2013-12-17

In this article, three different inorganic/organic doped carbon aerogel (CA) materials (Ni-CA, Pd-CA, and Ppy-CA) were, respectively, mixed with ionic liquid (IL) to form three stable composite films, which were used as enhanced elements for an integrated sensing platform to increase the surface area and to improve the electronic transmission rate. Subsequently, the effect of the materials performances such as adsorption, specific surface area and conductivity on electrochemistry for myoglobin (Mb) was discussed using N2 adsorption-desorption isotherm measurements, scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS). Moreover, they could act as sensors toward the detection of hydrogen peroxide (H2O2) with lower detection limits (1.68 μM, 1.02 μM, and 0.85 μM, for Ni-CA/IL/Mb-CPE, Pd-CA/IL/Mb-CPE, and Ppy-CA/IL/Mb-CPE, respectively) and smaller apparent Michaelis-Menten constants KM. The results indicated that the electroconductibility of the doped CA materials would become dominant, thus playing an important role in facilitating the electron transfer. Meanwhile, the synergetic effect with [BMIm]BF4 IL improved the capability of the composite inorganic/organic doped CA/IL matrix for protein immobilization. This work demonstrates the feasibility and the potential of a series of CA-based hybrid materials as biosensors, and further research and development are required to prepare other functional CAs and make them valuable for more extensive application in biosensing.

ANALOG I/O MODULE TEST SYSTEM BASED ON EPICS CA PROTOCOL AND ACTIVEX CA INTERFACE

DOE Office of Scientific and Technical Information (OSTI.GOV)

YENG,YHOFF,L.

2003-10-13

Analog input (ADC) and output (DAC) modules play a substantial role in device level control of accelerator and large experiment physics control system. In order to get the best performance some features of analog modules including linearity, accuracy, crosstalk, thermal drift and so on have to be evaluated during the preliminary design phase. Gain and offset error calibration and thermal drift compensation (if needed) may have to be done in the implementation phase as well. A natural technique for performing these tasks is to interface the analog VO modules and GPIB interface programmable test instruments with a computer, which canmore » complete measurements or calibration automatically. A difficulty is that drivers of analog modules and test instruments usually work on totally different platforms (vxworks VS Windows). Developing new test routines and drivers for testing instruments under VxWorks (or any other RTOS) platform is not a good solution because such systems have relatively poor user interface and developing such software requires substantial effort. EPICS CA protocol and ActiveX CA interface provide another choice, a PC and LabVIEW based test system. Analog 110 module can be interfaced from LabVIEW test routines via ActiveX CA interface. Test instruments can be controlled via LabVIEW drivers, most of which are provided by instrument vendors or by National Instruments. Labview also provides extensive data analysis and process functions. Using these functions, users can generate powerful test routines very easily. Several applications built for Spallation Neutron Source (SNS) Beam Loss Monitor (BLM) system are described in this paper.« less

Facilitating the inclusion of nonmarket values in Bureau of Land Management planning and project assessments—Final report

USGS Publications Warehouse

Huber, Chris; Richardson, Leslie

2016-11-23

Executive SummaryThis report summarizes the results of a series of field-based case studies conducted by the U.S. Geological Survey (USGS) to (1) evaluate the use of nonmarket values in Bureau of Land Management (BLM) planning and project assessments, (2) update existing technical resources for measuring those values, and (3) provide guidance to field staff on the use of nonmarket values. Four BLM pilot sites participated in this effort: Canyons of the Ancients National Monument in Colorado, Red Cliffs and Beaver Dam Wash National Conservation Areas in Utah, BLM’s Taos Field Office in New Mexico, and BLM's Tuscarora Field Office in Nevada. The focus of the case studies was on practical applications of nonmarket valuation. USGS worked directly with BLM field staff at the pilot sites to demonstrate the process of considering nonmarket values in BLM decisionmaking and document the questions, challenges, and opportunities that arise when tying economic language to projects.As part of this effort, a Web-based toolkit, available at https://my.usgs.gov/benefit-transfer/, was updated and expanded to help facilitate benefit transfers (that is, the use of existing economic data to quantify nonmarket values) and qualitative discussions of nonmarket values. A total of 53 new or overlooked nonmarket valuation studies comprising 494 nonmarket value estimates for various recreational activities and the preservation of threatened, endangered, and rare species were added to existing databases within this Benefit Transfer Toolkit. In addition, four meta-regression functions focused on hunting, wildlife viewing, fishing, and trail use recreation were developed and added to the Benefit Transfer Toolkit.Results of this effort demonstrate that there are two main roles for nonmarket valuation in BLM planning. The first is to improve the decisionmaking process by contributing to a more comprehensive comparison of economic benefits and cost when evaluating resource tradeoffs for National Environmental Policy Act analyses. The second is to use economic language and information on economic values, either qualitative or quantitative, to improve the ability to communicate the economic significance of the resources provided by BLM-managed lands. Findings also indicate that the use of existing economic data to quantify nonmarket values (that is, benefit transfer) poses unique challenges because of the scarcity of both resource data and existing valuation studies focused on resources and sites managed by BLM. This highlights the need for improvements in the collection of resource data at BLM sites, especially visitor use data, as well as an opportunity for BLM’s Socioeconomics Program to strategically identify priority areas, in terms of both resources and geographic locations, where primary valuation studies could be conducted and the results used for future benefit transfers. Finally, whereas qualitative discussions of nonmarket values do not facilitate the comparison of monetized values, they can provide a manageable next step forward in providing more comprehensive information on nonmarket values for BLM plans and project assessments. 

75 FR 14622 - Call for Nominations for Resource Advisory Councils

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-26

... expiring this year. The RACs provide advice and recommendations to the BLM on land use planning and management of the National System of Public Lands within their geographic areas. The BLM will accept public...

43 CFR 3602.49 - When will BLM issue a non-renewable contract?

Code of Federal Regulations, 2011 CFR

2011-10-01

... conflicting, purpose after mineral materials are removed; (2) The deposit of mineral materials may be... will include this information. (c) If your contract is in existence on December 24, 2001, BLM will...

Use of Multiple Linear Regression Models for Setting Water Quality Criteria for Copper: A Complementary Approach to the Biotic Ligand Model.

PubMed

Brix, Kevin V; DeForest, David K; Tear, Lucinda; Grosell, Martin; Adams, William J

2017-05-02

Biotic Ligand Models (BLMs) for metals are widely applied in ecological risk assessments and in the development of regulatory water quality guidelines in Europe, and in 2007 the United States Environmental Protection Agency (USEPA) recommended BLM-based water quality criteria (WQC) for Cu in freshwater. However, to-date, few states have adopted BLM-based Cu criteria into their water quality standards on a state-wide basis, which appears to be due to the perception that the BLM is too complicated or requires too many input variables. Using the mechanistic BLM framework to first identify key water chemistry parameters that influence Cu bioavailability, namely dissolved organic carbon (DOC), pH, and hardness, we developed Cu criteria using the same basic methodology used by the USEPA to derive hardness-based criteria but with the addition of DOC and pH. As an initial proof of concept, we developed stepwise multiple linear regression (MLR) models for species that have been tested over wide ranges of DOC, pH, and hardness conditions. These models predicted acute Cu toxicity values that were within a factor of ±2 in 77% to 97% of tests (5 species had adequate data) and chronic Cu toxicity values that were within a factor of ±2 in 92% of tests (1 species had adequate data). This level of accuracy is comparable to the BLM. Following USEPA guidelines for WQC development, the species data were then combined to develop a linear model with pooled slopes for each independent parameter (i.e., DOC, pH, and hardness) and species-specific intercepts using Analysis of Covariance. The pooled MLR and BLM models predicted species-specific toxicity with similar precision; adjusted R 2 and R 2 values ranged from 0.56 to 0.86 and 0.66-0.85, respectively. Graphical exploration of relationships between predicted and observed toxicity, residuals and observed toxicity, and residuals and concentrations of key input parameters revealed many similarities and a few key distinctions between the performances of the two models. The pooled MLR model was then applied to the species sensitivity distribution to derive acute and chronic criteria equations similar in form to the USEPA's current hardness-based criteria equations but with DOC, pH, and hardness as the independent variables. Overall, the MLR is less responsive to DOC than the BLM across a range of hardness and pH conditions but more responsive to hardness than the BLM. Additionally, at low and intermediate hardness, the MLR model is less responsive than the BLM to pH, but the two models respond comparably at high hardness. The net effect of these different response profiles is that under many typical water quality conditions, MLR- and BLM-based criteria are quite comparable. Indeed, conditions where the two models differ most (high pH/low hardness and low pH/high hardness) are relatively rare in natural aquatic systems. We suggest that this MLR-based approach, which includes the mechanistic foundation of the BLM but is also consistent with widely accepted hardness-dependent WQC in terms of development and form, may facilitate adoption of updated state-wide Cu criteria that more accurately account for the parameters influencing Cu bioavailability than current hardness-based criteria.

Psychophysiological Effects of Aging: Developing a Functional Age Index for Pilots: I. A Survey of the Pertinent Literature

DTIC Science & Technology

1977-04-01

wi ielt 1 t pait f tlieob cr iou It(’l ei(-t t lie t i(tio~logo~iCa I ag of sal tjeCts all to It I ns beenl litpothlesi zed that older. S11ii ject S...Talland, G.A. (Erd. ), H/uman .-Irilly-’MAN DEELOMENl’, :7781,19r. ed Bermri ~or. New York, Academic Pres,’ 196S. S. Bergnranl, M. : Clianrges of I

Bubble Memory Module.

DTIC Science & Technology

1980-12-01

I AD-A093 642 ROCKWELL INTERNATIONAL ANAHEIM CA AUOEISSTAE-T F/S V/2 I BU13LE MEMORY MODULE. (U) DEC 80 0 0 BOHNING. F J BECKER NASI -14174...Cde under Contract NASI -14174 Dist’m/o National Aeronautics and Space Administration Scientific and Technical Information BranchA 1980 J Approvod ior...9/ BUBBLE M MORY MODULE.(U) DEC 80 0 BHNING, F J BECKER NASI -14174 NCLASSIFIED CB-569/201 NASA-CR-3380 ML22-fllfllf ll l ff mmlmmmmm.l®fmmM EEmmEI

Targeting SOD1 induces synthetic lethal killing in BLM- and CHEK2-deficient colorectal cancer cells

PubMed Central

Sajesh, Babu V.; McManus, Kirk J.

2015-01-01

Cancer is a major cause of death throughout the world, and there is a large need for better and more personalized approaches to combat the disease. Over the past decade, synthetic lethal approaches have been developed that are designed to exploit the aberrant molecular origins (i.e. defective genes) that underlie tumorigenesis. BLM and CHEK2 are two evolutionarily conserved genes that are somatically altered in a number of tumor types. Both proteins normally function in preserving genome stability through facilitating the accurate repair of DNA double strand breaks. Thus, uncovering synthetic lethal interactors of BLM and CHEK2 will identify novel candidate drug targets and lead chemical compounds. Here we identify an evolutionarily conserved synthetic lethal interaction between SOD1 and both BLM and CHEK2 in two distinct cell models. Using quantitative imaging microscopy, real-time cellular analyses, colony formation and tumor spheroid models we show that SOD1 silencing and inhibition (ATTM and LCS-1 treatments), or the induction of reactive oxygen species (2ME2 treatment) induces selective killing within BLM- and CHEK2-deficient cells relative to controls. We further show that increases in reactive oxygen species follow SOD1 silencing and inhibition that are associated with the persistence of DNA double strand breaks, and increases in apoptosis. Collectively, these data identify SOD1 as a novel candidate drug target in BLM and CHEK2 cancer contexts, and further suggest that 2ME2, ATTM and LCS-1 are lead therapeutic compounds warranting further pre-clinical study. PMID:26318585

Targeting SOD1 induces synthetic lethal killing in BLM- and CHEK2-deficient colorectal cancer cells.

PubMed

Sajesh, Babu V; McManus, Kirk J

2015-09-29

Cancer is a major cause of death throughout the world, and there is a large need for better and more personalized approaches to combat the disease. Over the past decade, synthetic lethal approaches have been developed that are designed to exploit the aberrant molecular origins (i.e. defective genes) that underlie tumorigenesis. BLM and CHEK2 are two evolutionarily conserved genes that are somatically altered in a number of tumor types. Both proteins normally function in preserving genome stability through facilitating the accurate repair of DNA double strand breaks. Thus, uncovering synthetic lethal interactors of BLM and CHEK2 will identify novel candidate drug targets and lead chemical compounds. Here we identify an evolutionarily conserved synthetic lethal interaction between SOD1 and both BLM and CHEK2 in two distinct cell models. Using quantitative imaging microscopy, real-time cellular analyses, colony formation and tumor spheroid models we show that SOD1 silencing and inhibition (ATTM and LCS-1 treatments), or the induction of reactive oxygen species (2ME2 treatment) induces selective killing within BLM- and CHEK2-deficient cells relative to controls. We further show that increases in reactive oxygen species follow SOD1 silencing and inhibition that are associated with the persistence of DNA double strand breaks, and increases in apoptosis. Collectively, these data identify SOD1 as a novel candidate drug target in BLM and CHEK2 cancer contexts, and further suggest that 2ME2, ATTM and LCS-1 are lead therapeutic compounds warranting further pre-clinical study.

Influence of Li3BO3 additives on the Li+ conductivity and stability of Ca/Ta-substituted Li6.55(La2.95Ca0.05)(Zr1.5Ta0.5)O12 electrolytes

NASA Astrophysics Data System (ADS)

Zhang, L. C.; Yang, J. F.; Gao, Y. X.; Wang, X. P.; Fang, Q. F.; Chen, C. H.

2017-07-01

The cubic Ca/Ta-substituted Li6.55(La2.95Ca0.05)(Zr1.5Ta0.5)O12 (LLCZTO) electrolytes were synthesized at 800 °C with Li3BO3 as additives. The optimal amount of Li3BO3 and its influences on the microstructure, crystal structures, Li+ conductivity and the stability of the Li6.55(La2.95Ca0.05)(Zr1.5Ta0.5)O12 were studied by SEM, XRD and EIS. Among all the samples, when the molar ratio of Li3BO3 to the Li6.55(La2.95Ca0.05)(Zr1.5Ta0.5)O12 is 4:5, the highest Li+ conductivity of 1.33 × 10-4 S cm-1 at 30 °C is obtained. When the LLCZTO samples are exposed in air, the Li+ conductivity is deteriorated possibly owing to the side reactions between the LLCZTO and the H2O or CO2 in the air. The Li3BO3 addition can alleviate such deterioration of the Li+ conductivity.

43 CFR 3830.23 - What types of payment will BLM accept?

Code of Federal Regulations, 2014 CFR

2014-10-01

... Interior—Bureau of Land Management; (4) Valid credit card that is acceptable to the BLM; or (5) An... issuing institution of your check, negotiable instrument, or credit card refuses to pay and it is not...

43 CFR 3830.23 - What types of payment will BLM accept?

Code of Federal Regulations, 2012 CFR

2012-10-01

... Interior—Bureau of Land Management; (4) Valid credit card that is acceptable to the BLM; or (5) An... issuing institution of your check, negotiable instrument, or credit card refuses to pay and it is not...

43 CFR 3830.23 - What types of payment will BLM accept?

Code of Federal Regulations, 2013 CFR

2013-10-01

... Interior—Bureau of Land Management; (4) Valid credit card that is acceptable to the BLM; or (5) An... issuing institution of your check, negotiable instrument, or credit card refuses to pay and it is not...

43 CFR 3830.23 - What types of payment will BLM accept?

Code of Federal Regulations, 2011 CFR

2011-10-01

... Interior—Bureau of Land Management; (4) Valid credit card that is acceptable to the BLM; or (5) An... issuing institution of your check, negotiable instrument, or credit card refuses to pay and it is not...

76 FR 77477 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-13

... Receipt to collect information. Need and Use of the Information: Using forms FS-2400-1/BLM-5450-24, FS-2400-4 and FS-2400-8, FS and BLM will collect the name, address and tax identification number from...

78 FR 28240 - Call for Nominations for the Utah Resource Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-14

... expiring on January 12, 2014. The RAC provides advice and recommendations to the BLM on land use planning and management of the National System of Public Lands within Utah. The BLM will accept public...

78 FR 50088 - Notice of Availability of the Northwest Colorado Greater Sage-Grouse Draft Resource Management...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-16

... identified as having the highest conservation value to maintaining sustainable GRSG populations; include..., 2012. The BLM held four scoping open houses in January and February 2012. The BLM used public scoping...

Genetics Home Reference: Bloom syndrome

MedlinePlus

... are some genetic conditions more common in particular ethnic groups? Genetic Changes Mutations in the BLM gene cause Bloom syndrome . The BLM gene provides instructions for making a member of a protein family called RecQ helicases. Helicases are enzymes that attach ( ...

BLM germline and somatic PKMYT1 and AHCY mutations: Genetic variations beyond MYCN and prognosis in neuroblastoma.

PubMed

Novak, E M; Halley, N S; Gimenez, T M; Rangel-Santos, A; Azambuja, A M P; Brumatti, M; Pereira, P L; Vince, C S C; Giorgi, R R; Bendit, I; Cristofani, L M; Odone-Filho, V

2016-12-01

Neuroblastoma (NB) is the most common extra cranial solid tumor of childhood and often lethal in childhood. Clinical and biologic characteristics that are independently prognostic of outcome in NB are currently used for risk stratification to optimally the therapy. It includes age at diagnosis, International Neuroblastoma Staging System tumor histopathology and MYCN amplification. However, even in patients with theoretically good prognosis, such as localized tumor and non-amplified MYCN, either disease progress or recurrence may occur. Potential genetic determinants of this unfavorable behavior are not yet fully clarified. The presence of elevated expression of AHCY, PKMYT1, and BLM has accompanied poor prognosis MYCN-amplified neuroblastoma patients. Considering the potential implication of these genes on the clinical management of NB, we hypothesize that the identification of genetic variations may have significant impact during development of the recurrent or progressive disease. Using targeted DNA sequencing, we analyzed the mutation profiles of the genes PKMYT1, AHCY, and BLM in tumor samples of five patients with MYCN amplified and 15 MYCN non-amplified NB. In our study, BLM germline variants were detected in two patients with MYCN-non-amplified neuroblastoma. Our data allow us to hypothesize that, regardless of MYCN status, these mutations partially abolish BLM protein activity by impairing its ATPase and helicase activities. BLM mutations are also clinically relevant because BLM plays an important role in DNA damage repair and the maintenance of genomic integrity. We also found a novel variant in our cohort, PKMYT1 mutation localized in the C-terminal domain with effect unknown on NB. We hypothesize that this variant may affect the catalytic activity of PKMYT1 in NB, specifically when CDK1 is complexed to cyclins. The prognostic value of this mutation must be further investigated. Another mutation identified was a nonsynonymous variant in AHCY. This variant may be related to the slow progression of the disease, even in more aggressive cases. It affects the maintenance of the catalytic capacity of AHCY, leading to the consequent functional effects observed in the NB patients studied. In conclusion, our hypothesis may provide that mutations in BLM, AHCY and PKMYT1 genes found in children with MYCN-amplified or MYCN-non amplified neuroblastomas, may be associated with the prognosis of the disease. Copyright © 2016 Elsevier Ltd. All rights reserved.

Chromospherically active stars. III - HD 26337 = EI Eri: An RS CVn candidate for the Doppler-imaging technique

NASA Technical Reports Server (NTRS)

Fekel, Francis C.; Quigley, Robert; Gillies, Kim; Africano, John L.

1987-01-01

Spectroscopic observations of the chromospherically active G5 IV single-lined binary HD 26337 = EI Eri are presented. An orbital period of 1.94722 days is found for the star. It has moderately strong Ca II H and K emission and strong ultraviolet emission features, while H-alpha is a weak absorption feature that is variable in strength. The inclination of the system is 46 + or - 12 deg, and the unseen secondary is probably a late K or early M dwarf. The v sin i of the primary is 50 + or - 3 km/s, resulting in a minimum radius of 1.9 + or - 0.1 solar radius. The star is within the required limits for Doppler imaging. The primary is close to filling its Roche lobe, resulting in a strong constraint that the mass ratio is 2.6 or greater, with a primary mass of at least 1.4 solar mass. The distance to the system is estimated at 75 pc.

Range-wide assessment of livestock grazing across the sagebrush biome

USGS Publications Warehouse

Veblen, Kari E.; Pyke, David A.; Jones, Christopher A.; Casazza, Michael L.; Assal, Timothy J.; Farinha, Melissa A.

2011-01-01

Domestic livestock grazing occurs in virtually all sagebrush habitats and is a prominent disturbance factor. By affecting habitat condition and trend, grazing influences the resources required by, and thus, the distribution and abundance of sagebrush-obligate wildlife species (for example, sage-grouse Centrocercus spp.). Yet, the risks that livestock grazing may pose to these species and their habitats are not always clear. Although livestock grazing intensity and associated habitat condition may be known in many places at the local level, we have not yet been able to answer questions about use, condition, and trend at the landscape scale or at the range-wide scale for wildlife species. A great deal of information about grazing use, management regimes, and ecological condition exists at the local level (for individual livestock management units) under the oversight of organizations such as the Bureau of Land Management (BLM). However, the extent, quality, and types of existing data are unknown, which hinders the compilation, mapping, or analysis of these data. Once compiled, these data may be helpful for drawing conclusions about rangeland status, and we may be able to identify relationships between those data and wildlife habitat at the landscape scale. The overall objective of our study was to perform a range-wide assessment of livestock grazing effects (and the relevant supporting data) in sagebrush ecosystems managed by the BLM. Our assessments and analyses focused primarily on local-level management and data collected at the scale of BLM grazing allotments (that is, individual livestock management units). Specific objectives included the following: 1. Identify and refine existing range-wide datasets to be used for analyses of livestock grazing effects on sagebrush ecosystems. 2. Assess the extent, quality, and types of livestock grazing-related natural resource data collected by BLM range-wide (i.e., across allotments, districts and regions). 3. Compile and synthesize recommendations from federal and university rangeland science experts about how BLM might prioritize collection of different types of livestock grazing-related natural resource data. 4. Investigate whether range-wide datasets (Objective 1) could be used in conjunction with remotely sensed imagery to identify across broad scales (a) allotments potentially not meeting BLM Land Health Standards (LHS) and (b) allotments in which unmet standards might be attributable to livestock grazing. Objective 1: We identified four datasets that potentially could be used for analyses of livestock grazing effects on sagebrush ecosystems. First, we obtained the most current spatial data (typically up to 2007, 2008, or 2009) for all BLM allotments and compiled data into a coarse, topologically enforced dataset that delineated grazing allotment boundaries. Second, we obtained LHS evaluation data (as of 2007) for all allotments across all districts and regions; these data included date of most recent evaluation, BLM determinations of whether region-specific standards were met, and whether BLM deemed livestock to have contributed to any unmet standards. Third, we examined grazing records of three types: Actual Use (permittee-reported), Billed Use (BLM-reported), and Permitted Use (legally authorized). Finally, we explored the possibility of using existing Natural Resources Conservation Service (NRCS) Ecological Site Description (ESD) data to make up-to-date estimates of production and forage availability on BLM allotments. Objective 2: We investigated the availability of BLM livestock grazing-related monitoring data and the status of LHS across 310 randomly selected allotments in 13 BLM field offices. We found that, relative to other data types, the most commonly available monitoring data were Actual Use numbers (permittee-reported livestock numbers and season-of-use), followed by Photo Point, forage Utilization, and finally, Vegetation Trend measurement data. Data availability and frequency of data collection varied across allotments and field offices. Analysis of the BLM's LHS data indicated 67 percent of allotments analyzed were meeting standards. For those not meeting standards, livestock were considered the causal factor in 45 percent of cases (about 15 percent of all allotments). Objective 3: We sought input from 42 university and federal rangeland science experts about how best to prioritize rangeland monitoring activities associated with ascertaining livestock impacts on vegetation resources. When we presented a hypothetical scenario to these scientists and asked them to prioritize monitoring activities, the most common response was to measure ground and vegetation cover, a variable that in many cases (10 of 13 field offices sampled) BLM had already identified as a monitoring priority. Experts identified several other traditional (for example, photo points) and emerging approaches (for example, high-resolution aerial photography) to monitoring. Objective 4: We used spatial allotment data (described in Objective 1) and remotely sensed vegetation data (sagebrush cover, herbaceous vegetation cover, litter and bare soil) to assess differences in allotment LHS status ("Not met" vs. "Met"; if "Not met" - livestock-caused vs. not). We then developed logistic regression models, using vegetation variables to predict LHS status of BLM allotments in sagebrush steppe habitats in Wyoming and portions of Montana and Colorado. In general, we found that more consistent data collection at the local level might improve suitability of data for broad-scale analyses of livestock impacts. As is, data collection methodologies varied across field offices and States, and we did not find any local-level monitoring data (Actual Use, Utilization, Vegetation Trend) that had been collected consistently enough over time or space for range-wide analyses. Moreover, continued and improved emphasis on monitoring also may aid local management decisions, particularly with respect to effects of livestock grazing. Rangeland science experts identified ground cover as a high monitoring priority for assessing range condition and emphasized the importance of tracking livestock numbers and grazing dates. Ultimately, the most effective monitoring program may entail both increased data collection effort and the integration of alternative monitoring approaches (for example, remote sensing or monitoring teams). In the course of our study, we identified three additional datasets that could potentially be used for range-wide analyses: spatial allotment boundary data for all BLM allotments range-wide, LHS evaluations of BLM allotments, and livestock use data (livestock numbers and grazing dates). It may be possible to use these spatial datasets to help prioritize monitoring activities over the extensive land areas managed by BLM. We present an example of how we used spatial allotment boundary data and LHS data to test whether remotely sensed vegetation characteristics could be used to predict which allotments met or did not meet LHS. This approach may be further improved by the results of current efforts by BLM to test whether more intensive (higher resolution) LHS assessments more accurately describe land health status. Standardized data collection in more ecologically meaningful land units may improve our ability to use local-level data for broad-scale analyses.

43 CFR 3204.15 - May I withdraw my application for a noncompetitive lease?

Code of Federal Regulations, 2013 CFR

2013-10-01

... application for a noncompetitive lease? During the 30-day period after the competitive lease sale, BLM will... noncompetitive lease application in whole or in part at any time before BLM issues the lease. If a partial...

43 CFR 3204.15 - May I withdraw my application for a noncompetitive lease?

Code of Federal Regulations, 2011 CFR

2011-10-01

... application for a noncompetitive lease? During the 30-day period after the competitive lease sale, BLM will... noncompetitive lease application in whole or in part at any time before BLM issues the lease. If a partial...

43 CFR 3204.15 - May I withdraw my application for a noncompetitive lease?

Code of Federal Regulations, 2014 CFR

2014-10-01

... application for a noncompetitive lease? During the 30-day period after the competitive lease sale, BLM will... noncompetitive lease application in whole or in part at any time before BLM issues the lease. If a partial...

43 CFR 3204.15 - May I withdraw my application for a noncompetitive lease?

Code of Federal Regulations, 2012 CFR

2012-10-01

... application for a noncompetitive lease? During the 30-day period after the competitive lease sale, BLM will... noncompetitive lease application in whole or in part at any time before BLM issues the lease. If a partial...

78 FR 53477 - Second Call for Nominations to the Utah Resource Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-29

... positions with terms expiring on January 12, 2014. The RAC provides advice and recommendations to the BLM on land use planning and management of the National System of Public Lands within Utah. The BLM will...

76 FR 65530 - Alaska Native Claims Selection

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-21

... 26; Secs. 33, 35, and 36. Containing 3,689 acres. T. 21 N., R. 47 W., Sec. 34. Containing 457.50.... FOR FURTHER INFORMATION CONTACT: The BLM by phone at 907-271-5960 or by e-mail at ak.blm.conveyance...

77 FR 17091 - Notice of Availability of the Draft Environmental Impact Statement/Draft Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-23

... Availability in the Federal Register. The BLM will announce future meetings or hearings and any other public... Revised Franchise Agreement from the County of San Bernardino. The BLM and San Bernardino County are...

Long-term treatment with royal jelly improves bleomycin-induced pulmonary fibrosis in rats.

PubMed

Zargar, Hamid Reza; Hemmati, Ali Asghar; Ghafourian, Mehri; Arzi, Ardeshir; Rezaie, Anahita; Javad-Moosavi, Seyed Ali

2017-01-01

This study investigated the anti-fibrotic potential of royal jelly (RJ) powder against bleomycin-induced pulmonary fibrosis in rats. The rats were given RJ orally (25, 50, and 100 mg/kg per day) for 7 consecutive days before the administration of single intratracheal instillation of bleomycin (BLM) at 7.5 IU/kg. RJ doses were continued for 21 days after BLM exposure. Fibrotic changes in the lungs were studied by cell count and analysis of cytokine levels in the bronchoalveolar lavage fluid (BALF), histopathological examination, and assaying oxidative stress biomarkers in lung tissue. The results showed that BLM administration significantly increased the fibrotic changes, collagen content, and levels of malondialdehyde and decreased total thiol and glutathione peroxidase antioxidant contents in the rats' lung tissue. An increase in the level of cell counts and pro-inflammatory and pro-fibrotic cytokines such as TNF-α and TGF-β in BALF was observed. Also, it significantly decreased IFN-γ, an anti-fibrotic cytokine, in BALF. However, RJ (50 and 100 mg/kg) reversed all of these biochemical indices as well as histopathological alterations induced by BLM. The present study demonstrates that RJ, by its antioxidant and anti-inflammatory properties, attenuates oxidative damage and fibrosis induced by BLM.

The roles of WRN and BLM RecQ helicases in the Alternative Lengthening of Telomeres

PubMed Central

Mendez-Bermudez, Aaron; Hidalgo-Bravo, Alberto; Cotton, Victoria E.; Gravani, Athanasia; Jeyapalan, Jennie N.; Royle, Nicola J.

2012-01-01

Approximately 10% of all cancers, but a higher proportion of sarcomas, use the recombination-based alternative lengthening of telomeres (ALT) to maintain telomeres. Two RecQ helicase genes, BLM and WRN, play important roles in homologous recombination repair and they have been implicated in telomeric recombination activity, but their precise roles in ALT are unclear. Using analysis of sequence variation present in human telomeres, we found that a WRN– ALT+ cell line lacks the class of complex telomere mutations attributed to inter-telomeric recombination in other ALT+ cell lines. This suggests that WRN facilitates inter-telomeric recombination when there are sequence differences between the donor and recipient molecules or that sister-telomere interactions are suppressed in the presence of WRN and this promotes inter-telomeric recombination. Depleting BLM in the WRN– ALT+ cell line increased the mutation frequency at telomeres and at the MS32 minisatellite, which is a marker of ALT. The absence of complex telomere mutations persisted in BLM-depleted clones, and there was a clear increase in sequence homogenization across the telomere and MS32 repeat arrays. These data indicate that BLM suppresses unequal sister chromatid interactions that result in excessive homogenization at MS32 and at telomeres in ALT+ cells. PMID:22989712

The roles of WRN and BLM RecQ helicases in the Alternative Lengthening of Telomeres.

PubMed

Mendez-Bermudez, Aaron; Hidalgo-Bravo, Alberto; Cotton, Victoria E; Gravani, Athanasia; Jeyapalan, Jennie N; Royle, Nicola J

2012-11-01

Approximately 10% of all cancers, but a higher proportion of sarcomas, use the recombination-based alternative lengthening of telomeres (ALT) to maintain telomeres. Two RecQ helicase genes, BLM and WRN, play important roles in homologous recombination repair and they have been implicated in telomeric recombination activity, but their precise roles in ALT are unclear. Using analysis of sequence variation present in human telomeres, we found that a WRN- ALT+ cell line lacks the class of complex telomere mutations attributed to inter-telomeric recombination in other ALT+ cell lines. This suggests that WRN facilitates inter-telomeric recombination when there are sequence differences between the donor and recipient molecules or that sister-telomere interactions are suppressed in the presence of WRN and this promotes inter-telomeric recombination. Depleting BLM in the WRN- ALT+ cell line increased the mutation frequency at telomeres and at the MS32 minisatellite, which is a marker of ALT. The absence of complex telomere mutations persisted in BLM-depleted clones, and there was a clear increase in sequence homogenization across the telomere and MS32 repeat arrays. These data indicate that BLM suppresses unequal sister chromatid interactions that result in excessive homogenization at MS32 and at telomeres in ALT+ cells.

78 FR 19522 - Notice of Public Meeting: Resource Advisory Council to the Boise District, Bureau of Land...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-01

...-member Council advises the Secretary of the Interior, through the BLM, on a variety of planning and... contact the BLM Coordinator as provided above. Persons who use a telecommunications device for the deaf...

78 FR 5832 - Call for Nominations for Resource Advisory Councils

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-28

... Land Management, Correspondence, International, and Advisory Committee Office, 1849 C Street NW., MS.... The BLM will evaluate nominees based on their education, training, experience, and knowledge of the... nominee's qualifications. Simultaneous with this notice, BLM state offices will issue press releases...

Potential climate change impacts and the BLM Rio Puerco field office's transportation system : a technical report

DOT National Transportation Integrated Search

2015-03-01

This report provides information about potential climate change impacts in central New Mexico and their possible implications for the Bureau of Land Management (BLM) Rio Puerco Field Office (RPFO) transportation network. The report considers existing...

43 CFR 3264.10 - What must I submit to BLM after I complete a well?

Code of Federal Regulations, 2011 CFR

2011-10-01

... RESOURCE LEASING Reports-Drilling Operations § 3264.10 What must I submit to BLM after I complete a well... all logs; (c) Copies of all directional surveys; and (d) Copies of all mechanical, flow, reservoir...

78 FR 65703 - Notice of Availability of the Idaho and Southwestern Montana Greater Sage-Grouse Draft Land Use...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-01

... sustainable GRSG populations; include breeding, late brood-rearing and winter concentration areas. Preliminary... ended on March 23, 2012. The BLM held six scoping open houses in January and February 2012. The BLM used...

14-3-3ε Boosts Bleomycin-induced DNA Damage Response by Inhibiting the Drug-Resistant Activity of MVP

PubMed Central

Tang, Siwei; Bai, Chen; Yang, Pengyuan; Chen, Xian

2013-01-01

Major vault protein (MVP) is the predominant constituent of the vault particle, the largest known ribonuclear protein complex. Although emerging evidences have been establishing the links between MVP (vault) and multidrug resistance (MDR), little is known regarding exactly how the MDR activity of MVP is modulated during cellular response to drug-induced DNA damage (DDR). Bleomycin (BLM), an anti-cancer drug, induces DNA double-stranded breaks (DSBs) and consequently triggers the cellular DDR. Due to its physiological implications in hepatocellular carcinoma (HCC) and cell fate decision, 14-3-3ε was chosen as the pathway-specific bait protein to identify the critical target(s) responsible for HCC MDR. By using LC-MS/MS-based proteomic approach, MVP was first identified in the BLM-induced 14-3-3ε interactome formed in HCC cells. Biological characterization revealed that MVP possesses specific activity to promote the resistance to the BLM-induced DDR. On the other hand, 14-3-3ε enhances BLM-induced DDR by interacting with MVP. Mechanistic investigation further revealed that 14-3-3ε, in a phosphorylation-dependent manner, binds to the phosphorylated sites at both Thr52 and Ser864 of the monomer of MVP. Consequently, the phosphorylation-dependent binding between 14-3-3ε and MVP inhibits the drug-resistant activity of MVP for an enhanced DDR to BLM treatment. Our findings provide an insight into the mechanism underlying how the BLM-induced interaction between 14-3-3ε and MVP modulates MDR, implicating novel strategy to overcome the chemotherapeutic resistance through interfering specific protein-protein interactions. PMID:23590642

Semiconductor particles in bilayer lipid membranes. Formation, characterization, and photoelectrochemistry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, X.K.; Baral, S.B.; Rolandi, R.

Bilayer lipid membranes (BLMs) have been formed from bovine brain phosphatidylserine (PS), glyceryl monooleate (GMO), and a ploymerizable surfactant, (n-C/sub 15/H/sub 31/CO/sub 2/(CH/sub 2/))/sub 2/N/sup +/(CH/sub 3/)CH/sub 2/C/sub 6/H/sub 4/CH==CH/sub 2/Cl/sup -/(STYRS). These BLMs were then used to provide matrices for the in situ generation of microcrystalline CdS, CuS, Cu/sub 2/S, PbS, ZnS, HgS, and In/sub 2/S/sub 3/. Semiconductors were formed by injecting appropriate metal ion precursors and H/sub 2/S into the bathing solutions on opposite sides of the BLM. Their presence was established by voltage-dependent capacitance measurements, absorption spectroscopy, and optical microscopy. Subsequent to the injection of H/sub 2/S,more » the first observable change was the appearance of fairly uniform white dots on the black film. These dots rapidly moved around and grew in size, forming islands that then merged with themselves and with a second generation of dots, which ultimately led to a continuous film that continued to grow in thickness. Film formation and growth were monitored by simultaneous optical thickness and capacitance measurements. These data were treated in terms of an equivalent R-C circuit and allowed for the assessment of the semiconductor penetration depth into the BLM. This value for a GMO-BLM-supported In/sub 2/S/sub 3/ film was determined to be 24 A. Bandgap excitation, by nanosecond-pulsed or continuous illumination of the BLM-supported semiconductor film, led to observable photoelectric effects. Visible light (lambda > 350 nm) excitation into STYRS-BLM-supported CdS led to polymerization of the styrene moiety of STYRS. BLM-supported semiconductors remained stable for days.« less

Comparative study of two models of combined pulmonary fibrosis and emphysema in mice.

PubMed

Zhang, Wan-Guang; Wu, Si-Si; He, Li; Yang, Qun; Feng, Yi-Kuan; Chen, Yue-Tao; Zhen, Guo-Hua; Xu, Yong-Jian; Zhang, Zhen-Xiang; Zhao, Jian-Ping; Zhang, Hui-Lan

2017-04-01

Combined pulmonary fibrosis and emphysema (CPFE) is an "umbrella term" encompassing emphysema and pulmonary fibrosis, but its pathogenesis is not known. We established two models of CPFE in mice using tracheal instillation with bleomycin (BLM) or murine gammaherpesvirus 68 (MHV-68). Experimental mice were divided randomly into four groups: A (normal control, n=6), B (emphysema, n=6), C (emphysema+MHV-68, n=24), D (emphysema+BLM, n=6). Group C was subdivided into four groups: C1 (sacrificed on day 367, 7 days after tracheal instillation of MHV-68); C2 (day 374; 14days); C3 (day 381; 21days); C4 (day 388; 28days). Conspicuous emphysema and interstitial fibrosis were observed in BLM and MHV-68 CPFE mouse models. However, BLM induced diffuse pulmonary interstitial fibrosis with severely diffuse pulmonary inflammation; MHV-68 induced relatively modest inflammation and fibrosis, and the inflammation and fibrosis were not diffuse, but instead around bronchioles. Inflammation and fibrosis were detectable in the day-7 subgroup and reached a peak in the day-28 subgroup in the emphysema + MHV-68 group. Levels of macrophage chemoattractant protein-1, macrophage inflammatory protein-1α, interleukin-13, and transforming growth factor-β1 in bronchoalveolar lavage fluid were increased significantly in both models. Percentage of apoptotic type-2 lung epithelial cells was significantly higher; however, all four types of cytokine and number of macrophages were significantly lower in the emphysema+MHV-68 group compared with the emphysema +BLM group. The different changes in pathology between BLM and MHV-68 mice models demonstrated different pathology subtypes of CPFE: macrophage infiltration and apoptosis of type-II lung epithelial cells increased with increasing pathology score for pulmonary fibrosis. Copyright © 2017 Elsevier GmbH. All rights reserved.

Ferrocenyl-doped silica nanoparticles as an immobilized affinity support for electrochemical immunoassay of cancer antigen 15-3.

PubMed

Hong, Chenglin; Yuan, Ruo; Chai, Yaqin; Zhuo, Ying

2009-02-09

The aim of this study is to elaborate a simple and sensitive electrochemical immunoassay using ferrocenecarboxylic (Fc-COOH)-doped silica nanoparticles (SNPs) as an immobilized affinity support for cancer antigen 15-3 (CA 15-3) detection. The Fc-COOH-doped SNPs with redox-active were prepared by using a water-in-oil microemulsion method. The use of colloidal silica could prevent the leakage of Fc-COOH and were easily modified with trialkoxysilane reagents for covalent conjugation of CA 15-3 antibodies (anti-CA 15-3). The Fc-COOH-doped SNPs were characterized by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The fabrication process of the electrochemical immunosensor was demonstrated by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Under optimal conditions, the developed immunosensor showed good linearity at the studied concentration range of 2.0-240 UmL(-1) with a coefficient 0.9986 and a detection limit of 0.64 UmL(-1) at S/N=3.

77 FR 3789 - Call for Nominations for Resource Advisory Councils

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-25

..., Correspondence, International, and Advisory Committee Office, 1849 C Street NW., MS-MIB 5070, Washington, DC.... The BLM will evaluate nominees based on their education, training, experience, and knowledge of the... nominee's qualifications. Simultaneous with this notice, BLM state offices will issue press releases...

ACHP | News

Science.gov Websites

Working with Section 106 Federal, State, & Tribal Programs Training & Education Publications Search skip specific nav links Home arrow News arrow October 21, 2013 ACHP Provides 106 Training to the BLM-ACHP partnership, the ACHP liaison to the BLM, Nancy Brown, provided the training free of

78 FR 45266 - Notice of Intent To Prepare a Resource Management Plan for the Oklahoma, Kansas, and Texas...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-26

... decisions in the RMP will apply to the surface and subsurface estate managed by the BLM and the BIA. The BLM... realty, hydrology, soils, livestock grazing, recreation, sociology, and economics. Authority: 40 CFR 1501...

BLM forest lands report -- 2006 status and condition

Treesearch

Tim Bottomley; Jim Menlove

2006-01-01

The Bureau of Land Management (BLM), an agency within the U.S. Department of the Interior (DOI), administers over 261 million surface acres of public land in the western United States, including Alaska. Approximately 69 million acres, or 26 percent, are classified as forested.

75 FR 80839 - Notice of Realty Action: Direct Sale of Public Lands in Lane County, OR

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-23

... Environmental Policy Act Adequacy (DOI-BLM-OR-E050-2010-0006- DNA) that was approved on April 14, 2010. There... documentation, is available for review at the BLM Eugene District Office, 3106 Pierce Parkway, Suite E...

78 FR 34611 - Oil and Gas; Hydraulic Fracturing on Federal and Indian Lands

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-10

...On May 11, 2012, the Bureau of Land Management (BLM) published in the Federal Register a proposed rule to regulate hydraulic fracturing on Federal and Indian land. Due to the complexity of the rule and the issues surrounding it, the BLM extended the comment period for 60 days beyond the end of the initial comment period. On May 24, 2013, the BLM published a supplemental notice of proposed rulemaking and request for comment. Key issues in the revised proposed rule include: the use of an expanded set of cement evaluation tools to help ensure that usable water zones have been isolated and protected from contamination and more detailed guidance on how trade secrets claims will be handled. The revised proposed rule would also provide opportunities for the BLM to coordinate standards and processes with individual States and tribes to reduce administrative costs and improve efficiency. This notice extends the public comment period on the revised proposed rule for 60 days beyond the initial comment period.

Characterization of the Caenorhabditis elegans HIM-6/BLM helicase: unwinding recombination intermediates.

PubMed

Jung, Hana; Lee, Jin A; Choi, Seoyoon; Lee, Hyunwoo; Ahn, Byungchan

2014-01-01

Mutations in three human RecQ genes are implicated in heritable human syndromes. Mutations in BLM, a RecQ gene, cause Bloom syndrome (BS), which is characterized by short stature, cancer predisposition, and sensitivity to sunlight. BLM is a RecQ DNA helicase that, with interacting proteins, is able to dissolve various DNA structures including double Holliday junctions. A BLM ortholog, him-6, has been identified in Caenorhabditis elegans, but little is known about its enzymatic activities or its in vivo roles. By purifying recombinant HIM-6 and performing biochemical assays, we determined that the HIM-6 has DNA-dependent ATPase activity HIM-6 and helicase activity that proceeds in the 3'-5' direction and needs at least five 3' overhanging nucleotides. HIM-6 is also able to unwind DNA structures including D-loops and Holliday junctions. Worms with him-6 mutations were defective in recovering the cell cycle arrest after HU treatment. These activities strongly support in vivo roles for HIM-6 in processing recombination intermediates.

Characterization of the Caenorhabditis elegans HIM-6/BLM Helicase: Unwinding Recombination Intermediates

PubMed Central

Choi, Seoyoon; Lee, Hyunwoo; Ahn, Byungchan

2014-01-01

Mutations in three human RecQ genes are implicated in heritable human syndromes. Mutations in BLM, a RecQ gene, cause Bloom syndrome (BS), which is characterized by short stature, cancer predisposition, and sensitivity to sunlight. BLM is a RecQ DNA helicase that, with interacting proteins, is able to dissolve various DNA structures including double Holliday junctions. A BLM ortholog, him-6, has been identified in Caenorhabditis elegans, but little is known about its enzymatic activities or its in vivo roles. By purifying recombinant HIM-6 and performing biochemical assays, we determined that the HIM-6 has DNA-dependent ATPase activity HIM-6 and helicase activity that proceeds in the 3'-5' direction and needs at least five 3' overhanging nucleotides. HIM-6 is also able to unwind DNA structures including D-loops and Holliday junctions. Worms with him-6 mutations were defective in recovering the cell cycle arrest after HU treatment. These activities strongly support in vivo roles for HIM-6 in processing recombination intermediates. PMID:25036527

Preliminary cellular-automata forecast of permit activity from 1998 to 2010, Idaho and Western Montana

USGS Publications Warehouse

Raines, G.L.; Zientek, M.L.; Causey, J.D.; Boleneus, D.E.

2002-01-01

For public land management in Idaho and western Montana, the U.S. Forest Service (USFS) has requested that the U.S. Geological Survey (USGS) predict where mineral-related activity will occur in the next decade. Cellular automata provide an approach to simulation of this human activity. Cellular automata (CA) are defined by an array of cells, which evolve by a simple transition rule, the automaton. Based on exploration trends, we assume that future exploration will focus in areas of past exploration. Spatial-temporal information about mineral-related activity, that is permits issued by USFS and Bureau of Land Management (BLM) in the last decade, and spatial information about undiscovered resources, provide a basis to calibrate a CA. The CA implemented is a modified annealed voting rule that simulates mineral-related activity with spatial and temporal resolution of 1 mi2 and 1 year based on activity from 1989 to 1998. For this CA, the state of the economy and exploration technology is assumed constant for the next decade. The calibrated CA reproduces the 1989-1998-permit activity with an agreement of 94%, which increases to 98% within one year. Analysis of the confusion matrix and kappa correlation statistics indicates that the CA underestimates high activity and overestimates low activity. Spatially, the major differences between the actual and calculated activity are that the calculated activity occurs in a slightly larger number of small patches and is slightly more uneven than the actual activity. Using the calibrated CA in a Monte Carlo simulation projecting from 1998 to 2010, an estimate of the probability of mineral activity shows high levels of activity in Boise, Caribou, Elmore, Lincoln, and western Valley counties in Idaho and Beaverhead, Madison, and Stillwater counties in Montana, and generally low activity elsewhere. ?? 2002 International Association for Mathematical Geology.

Acute myeloid leukaemia after treatment for acute lymphoblastic leukaemia in girl with Bloom syndrome

PubMed Central

Adams, Madeleine; Jenney, Meriel; Lazarou, Laz; White, Rhian; Birdsall, Sanda; Staab, Timo; Schindler, Detlev; Meyer, Stefan

2014-01-01

Bloom syndrome (BS) is an inherited genomic instability disorder caused by disruption of the BLM helicase and confers an extreme cancer predisposition. Here we report on a girl with BS who developed acute lymphoblastic leukaemia (ALL) at age nine, and treatment-related acute myeloid leukaemia (t-AML) aged 12. She was compound heterozygous for the novel BLM frameshift deletion c.1624delG and the previously described c.3415C>T nonsense mutation. Two haematological malignancies in a child with BS imply a fundamental role for BLM for normal haematopoiesis, in particular in the presence of genotoxic stress. PMID:24932421

43 CFR 3900.20 - Appealing the BLM's decision.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Appealing the BLM's decision. 3900.20 Section 3900.20 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) OIL SHALE MANAGEMENT-GENERAL Oil Shale...

43 CFR 3900.20 - Appealing the BLM's decision.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Appealing the BLM's decision. 3900.20 Section 3900.20 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR RANGE MANAGEMENT (4000) OIL SHALE MANAGEMENT-GENERAL Oil Shale...

43 CFR 3501.20 - If BLM approves my application for a use authorization under this part, when does it become...

Code of Federal Regulations, 2013 CFR

2013-10-01

... MINERALS MANAGEMENT (3000) LEASING OF SOLID MINERALS OTHER THAN COAL AND OIL SHALE Leasing of Solid Minerals Other Than Coal and Oil Shale-General § 3501.20 If BLM approves my application for a use...

43 CFR 3501.20 - If BLM approves my application for a use authorization under this part, when does it become...

Code of Federal Regulations, 2012 CFR

2012-10-01

... MINERALS MANAGEMENT (3000) LEASING OF SOLID MINERALS OTHER THAN COAL AND OIL SHALE Leasing of Solid Minerals Other Than Coal and Oil Shale-General § 3501.20 If BLM approves my application for a use...

43 CFR 3501.20 - If BLM approves my application for a use authorization under this part, when does it become...

Code of Federal Regulations, 2011 CFR

2011-10-01

... MINERALS MANAGEMENT (3000) LEASING OF SOLID MINERALS OTHER THAN COAL AND OIL SHALE Leasing of Solid Minerals Other Than Coal and Oil Shale-General § 3501.20 If BLM approves my application for a use...

43 CFR 3900.20 - Appealing the BLM's decision.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Appealing the BLM's decision. 3900.20 Section 3900.20 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) OIL SHALE MANAGEMENT-GENERAL Oil Shale...

43 CFR 3900.20 - Appealing the BLM's decision.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Appealing the BLM's decision. 3900.20 Section 3900.20 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) OIL SHALE MANAGEMENT-GENERAL Oil Shale...

43 CFR 3501.20 - If BLM approves my application for a use authorization under this part, when does it become...

Code of Federal Regulations, 2014 CFR

2014-10-01

... MINERALS MANAGEMENT (3000) LEASING OF SOLID MINERALS OTHER THAN COAL AND OIL SHALE Leasing of Solid Minerals Other Than Coal and Oil Shale-General § 3501.20 If BLM approves my application for a use...

43 CFR 3910.32 - Environmental analysis.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Environmental analysis. 3910.32 Section 3910.32 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND... Licenses § 3910.32 Environmental analysis. (a) Before the BLM will issue an exploration license, the BLM...

78 FR 39008 - Renewal of Approved Information Collection

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-28

... enables the BLM to manage Federal coal resources in accordance with applicable statutes. The Office of...: This collection enables the BLM to learn the extent and qualities of Federal coal resources; evaluate... lessees to acquire and hold Federal coal leases; and ensure lessee compliance with applicable statutes...

77 FR 20409 - Notice of Amendment to the Notice of Intent To Prepare an Environmental Impact Statement for the...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-04

.... Although the plan amendments will only apply to BLM- managed lands, the BLM will develop and analyze in its... and realty, hydrology, soils, sociology and economics. Authority: 40 CFR 1501.7 and 43 CFR 1610.2...

78 FR 40494 - Renewal of Approved Information Collection

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-05

... that assists the BLM in managing operations authorized by the mining laws, in preventing unnecessary or... Mining Law (43 CFR subpart 3809). Forms: Form 3809-1, Surface Management Surety Bond; Form 3809-2... enables the BLM to determine whether operators and mining claimants are meeting their responsibility...

Using biotic ligand models to predict metal toxicity in mineralized systems

USGS Publications Warehouse

Smith, Kathleen S.; Balistrieri, Laurie S.; Todd, Andrew S.

2015-01-01

The biotic ligand model (BLM) is a numerical approach that couples chemical speciation calculations with toxicological information to predict the toxicity of aquatic metals. This approach was proposed as an alternative to expensive toxicological testing, and the U.S. Environmental Protection Agency incorporated the BLM into the 2007 revised aquatic life ambient freshwater quality criteria for Cu. Research BLMs for Ag, Ni, Pb, and Zn are also available, and many other BLMs are under development. Current BLMs are limited to ‘one metal, one organism’ considerations. Although the BLM generally is an improvement over previous approaches to determining water quality criteria, there are several challenges in implementing the BLM, particularly at mined and mineralized sites. These challenges include: (1) historically incomplete datasets for BLM input parameters, especially dissolved organic carbon (DOC), (2) several concerns about DOC, such as DOC fractionation in Fe- and Al-rich systems and differences in DOC quality that result in variations in metal-binding affinities, (3) water-quality parameters and resulting metal-toxicity predictions that are temporally and spatially dependent, (4) additional influences on metal bioavailability, such as multiple metal toxicity, dietary metal toxicity, and competition among organisms or metals, (5) potential importance of metal interactions with solid or gas phases and/or kinetically controlled reactions, and (6) tolerance to metal toxicity observed for aquatic organisms living in areas with elevated metal concentrations.

Modulation of cytokine and nitric oxide by mesenchymal stem cell transfer in lung injury/fibrosis

PubMed Central

2010-01-01

Background No effective treatment for acute lung injury and fibrosis currently exists. Aim of this study was to investigate the time-dependent effect of bone marrow-derived mesenchymal stem cells (BMDMSCs) on bleomycin (BLM)-induced acute lung injury and fibrosis and nitric oxide metabolites and inflammatory cytokine production. Methods BMDMSCs were transferred 4 days after BLM inhalation. Wet/dry ratio, bronchoalveolar lavage cell profiles, histologic changes and deposition of collagen were analyzed. Results Nitrite, nitrate and cytokines were measured weekly through day 28. At day 7, the wet/dry ratio, neutrophilic inflammation, and amount of collagen were elevated in BLM-treated rats compared to sham rats (p = 0.05-0.002). Levels nitrite, nitrate, IL-1β, IL-6, TNF-α, TGF-β and VEGF were also higher at day 7 (p < 0.05). Degree of lymphocyte and macrophage infiltration increased steadily over time. BMDMSC transfer significantly reduced the BLM-induced increase in wet/dry ratio, degree of neutrophilic infiltration, collagen deposition, and levels of the cytokines, nitrite, and nitrate to those in sham-treated rats (p < 0.05). Fluorescence in situ hybridization localized the engrafted cells to areas of lung injury. Conclusion Systemic transfer of BMDMSCs effectively reduced the BLM-induced lung injury and fibrosis through the down-regulation of nitric oxide metabolites, and proinflammatory and angiogenic cytokines. PMID:20137099

Water Quality Criteria for Copper Based on the BLM Approach in the Freshwater in China

PubMed Central

Zhang, Yahui; Zang, Wenchao; Qin, Lumei; Zheng, Lei; Cao, Ying; Yan, Zhenguang; Yi, Xianliang; Zeng, Honghu; Liu, Zhengtao

2017-01-01

The bioavailability and toxicity of metals to aquatic organisms are highly dependent on water quality parameters in freshwaters. The biotic ligand model (BLM) for copper is an approach to generate the water quality criteria (WQC) with water chemistry in the ambient environment. However, few studies were carried out on the WQCs for copper based on the BLM approach in China. In the present study, the toxicity for copper to native Chinese aquatic organisms was conducted and the published toxicity data with water quality parameters to Chinese aquatic species were collected to derive the WQCs for copper by the BLM approach. The BLM-based WQCs (the criterion maximum criteria (CMC) and the criterion continuous concentration (CCC)) for copper in the freshwater for the nation and in the Taihu Lake were obtained. The CMC and CCC values for copper in China were derived to be 1.391 μg/L and 0.495 μg/L, respectively, and the CMC and CCC in the Taihu Lake were 32.194 μg/L and 9.697 μg/L. The high concentration of dissolved organic carbon might be a main reason which resulted in the higher WQC values in the Taihu Lake. The WQC of copper in the freshwater would provide a scientific foundation for water quality standards and the environment risk assessment in China. PMID:28166229

75 FR 72837 - Eastern States: Filing of Plats of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-26

...] Eastern States: Filing of Plats of Survey AGENCY: Bureau of Land Management, Interior. ACTION: Notice of... (BLM) will file the plats of survey of the lands described below in the BLM-Eastern States office in... INFORMATION CONTACT: Bureau of Land Management-Eastern States, 7450 Boston Boulevard, Springfield, Virginia...

75 FR 4104 - Notice of Reestablishment of the National Historic Oregon Trail Interpretive Center Advisory Board

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-26

... Reestablishment of the National Historic Oregon Trail Interpretive Center Advisory Board AGENCY: Bureau of Land... has reestablished the Bureau of Land Management's (BLM) National Historic Oregon Trail Interpretive... participation between the BLM and the Oregon Trail Preservation Trust, ensuring a financially secure, world...

Building Partnerships to Monitor the Conditions of Streams and Rivers on Public Lands

EPA Science Inventory

The Bureau of Land Management (BLM), in collaboration with the U.S. Environmental Protection Agency (EPA), will conduct its first Western Rivers and Streams Assessment (WRSA), a survey of the condition of BLM streams and rivers throughout the contiguous western U.S. The objective...

Emerging ecological datasets with application for modeling North American dust emissions

USDA-ARS?s Scientific Manuscript database

In 2011 the US Bureau of Land Management (BLM) established the Assessment, Inventory and Monitoring (AIM) program to monitor the condition of BLM land and to provide data to support evidence-based management of multi-use public lands. The monitoring program shares core data collection methods with t...

78 FR 25204 - Segregation of Lands-Renewable Energy

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-30

....L13400000] RIN 1004-AE19 Segregation of Lands--Renewable Energy AGENCY: Bureau of Land Management, Interior... pending solar or wind renewable energy generation project, or for public lands identified by the BLM under... consideration of renewable energy ROWs. As explained below, the BLM seeks to avoid the delays and uncertainty...

43 CFR 3266.10 - Will BLM disclose information I submit under these regulations?

Code of Federal Regulations, 2012 CFR

2012-10-01

... public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 of this title may be made available for inspection without... submit under these regulations? All Federal and Indian data and information submitted to the BLM are...

43 CFR 3255.10 - Will BLM disclose information I submit under these regulations?

Code of Federal Regulations, 2012 CFR

2012-10-01

... covering public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 may be made available for inspection without a Freedom... submit under these regulations? All Federal and Indian data and information submitted to the BLM are...

43 CFR 3255.10 - Will BLM disclose information I submit under these regulations?

Code of Federal Regulations, 2013 CFR

2013-10-01

... covering public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 may be made available for inspection without a Freedom... submit under these regulations? All Federal and Indian data and information submitted to the BLM are...

43 CFR 3266.10 - Will BLM disclose information I submit under these regulations?

Code of Federal Regulations, 2013 CFR

2013-10-01

... public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 of this title may be made available for inspection without... submit under these regulations? All Federal and Indian data and information submitted to the BLM are...

43 CFR 2806.23 - How will the BLM calculate my rent for linear rights-of-way the Per Acre Rent Schedule covers?

Code of Federal Regulations, 2011 CFR

2011-10-01

... multiplying the rent per acre for the appropriate county (or other geographical area) zone from the current... (the length of time for which the holder is paying rent). (b) The BLM will phase-in the initial...

77 FR 64350 - State of Arizona Resource Advisory Council Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-19

.... ACTION: Notice of Public Meetings. SUMMARY: In accordance with the Federal Land Policy and Management Act..., through the BLM, on a variety of planning and management issues associated with public land management in Arizona. Planned agenda items include: a welcome and introduction of Council members; BLM State Director's...

Preliminary Results from the BLM’s Western Rivers and Streams Assessment

EPA Science Inventory

The Bureau of Land Management (BLM), in collaboration with the U.S. Environmental Protection Agency, is conducting its first Western Rivers and Streams Assessment (WRSA), a survey of the condition of BLM streams and rivers throughout the contiguous western U.S. The WRSA will answ...

75 FR 21345 - Notice of Meeting, Southeast Oregon Resource Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-23

... District; information below. FOR FURTHER INFORMATION CONTACT: Mark Wilkening, Public Affairs Officer, BLM... members please contact Mark Wilkening, Public Affairs Officer, BLM Vale District Office, 100 Oregon Street.... ACTION: Notice of Public Meeting. SUMMARY: Pursuant to the Federal Land Policy and Management Act and the...

75 FR 10813 - Notice of Public Meeting, Southeast Oregon Resource Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-09

... update, litigation updates, update on the BLM sagebrush/sage-grouse teams, Phase II Blue Mountain Forest...] Notice of Public Meeting, Southeast Oregon Resource Advisory Council AGENCY: Bureau of Land Management... (BLM) Southeast Oregon Resource Advisory Council (SEORAC) will meet as indicated below: DATES: The...

43 CFR 2568.40 - Does BLM have the authority to ask me for the information required in these regulations?

Code of Federal Regulations, 2011 CFR

2011-10-01

... AK-2561-10. BLM uses this information to determine if using the public lands is appropriate. You must... follows: 28 hours per response to fill out form AK-2561-10. These estimates include the time for reviewing...

43 CFR 2568.40 - Does BLM have the authority to ask me for the information required in these regulations?

Code of Federal Regulations, 2013 CFR

2013-10-01

... AK-2561-10. BLM uses this information to determine if using the public lands is appropriate. You must... follows: 28 hours per response to fill out form AK-2561-10. These estimates include the time for reviewing...

43 CFR 2568.40 - Does BLM have the authority to ask me for the information required in these regulations?

Code of Federal Regulations, 2014 CFR

2014-10-01

... AK-2561-10. BLM uses this information to determine if using the public lands is appropriate. You must... follows: 28 hours per response to fill out form AK-2561-10. These estimates include the time for reviewing...

43 CFR 2568.40 - Does BLM have the authority to ask me for the information required in these regulations?

Code of Federal Regulations, 2012 CFR

2012-10-01

... AK-2561-10. BLM uses this information to determine if using the public lands is appropriate. You must... follows: 28 hours per response to fill out form AK-2561-10. These estimates include the time for reviewing...

43 CFR 3137.23 - What must I include in my NPR-A unitization application?

Code of Federal Regulations, 2011 CFR

2011-10-01

... operations may reduce impacts compared to individual lease operations; (g) A discussion of the proposed... mineral estate, you must explain how the methodology takes into account reservoir heterogeneity and area variation in reservoir producibility; and (h) Other documentation BLM may request. BLM may require...

AIM-Monitoring: a component of the BLM assessment, inventory, and monitoring strategy

USDA-ARS?s Scientific Manuscript database

The “BLM Assessment, Inventory, and Monitoring Strategy for Integrated Renewable Resources Management” (AIM Strategy) was completed in 2011 in response to a request from the Office of Management and Budget. The strategy describes an approach for integrated, cross-program assessment, inventory, and m...

77 FR 35422 - Filing of Plats of Survey, Wyoming

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-13

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLWY-957400-12-L14200000-BJ0000] Filing of Plats of Survey, Wyoming AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: The Bureau of Land Management (BLM) has filed the plats of survey of the lands described below in the BLM...

77 FR 46112 - Call for Nominations for Advisory Groups, Oregon/Washington

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-02

... Steens Mountain Advisory Council. Citizens who serve on these groups provide advice and recommendations to the BLM on land use planning and management of the National System of Public Lands within their geographic areas and management options for a specific National Landscape Conservation System site. The BLM...

43 CFR 3262.13 - May BLM require me to follow a well spacing program?

Code of Federal Regulations, 2013 CFR

2013-10-01

... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Conducting Drilling Operations § 3262.13 May BLM require me to follow a well spacing... spacing: (a) Hydrologic, geologic, and reservoir characteristics of the field, minimizing well...

43 CFR 3262.13 - May BLM require me to follow a well spacing program?

Code of Federal Regulations, 2014 CFR

2014-10-01

... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Conducting Drilling Operations § 3262.13 May BLM require me to follow a well spacing... spacing: (a) Hydrologic, geologic, and reservoir characteristics of the field, minimizing well...

43 CFR 3262.13 - May BLM require me to follow a well spacing program?

Code of Federal Regulations, 2012 CFR

2012-10-01

... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Conducting Drilling Operations § 3262.13 May BLM require me to follow a well spacing... spacing: (a) Hydrologic, geologic, and reservoir characteristics of the field, minimizing well...

Support Equipment Selection Analysis (SESA) for the Navy Standard Airborne Computer Set (AN/AYK-14(V)).

DTIC Science & Technology

1980-11-17

111 - rb’" /JI- D.1 F. 1A.’ E I IJ~Ei]JF J I -126- HAEC-92-138 0 cz-: 00 CA a +. 9 11 11 118 0 rA. a , +, +.~ ’ q y 1 wI ~ 3- An~ii .03 -. VDI . 𔃾’ -127...32 - 2048 10 MHz +5 v - - 32 1024 10 MUz +5 v - lb - 1024 25 MHz +.76 v 224 - - 256 500 kHz +5 v (TTL) - 32 32 256 250 kHz +28 v - 32 32 286 250

Certification Study of a Derivative Model of a Small Jet Transport Airplane Using a Piloted Research Simulator

DTIC Science & Technology

1977-06-01

RESEARCH SIMULATOR • RAYMOND 0. FORREST SYSTEMS RESEARCH AND DEVELOPMENT SERVICE FEDERAL AVIATION ADMINISTRATION AMES RESEARCH CENTER MOFFE1T FIELD ...25 M o f f e t t Field , CA 94035 13. T ype of Repor t and P.r.od Co o er ed 12 . Sponsorrng Ar en cy Na me and Add eis ___________ U . S...dynamic stability derivatives of a complete airplane . The method utilizes potential flow theory to compute the surface flow fields and pressures on any

Final Environmental Impact Statement (EIS) for the Space Nuclear Thermal Propulsion (SNTP) Program. Sanitized Version

DTIC Science & Technology

1991-09-19

Company) in Torrance, CA; (8) Gnmman Space Electronics Division in Bethpage, NY; (9) Raytheon Services Nevada (RSN) in Las Vegas, NV; (10) Reynolds...10 to 20 minutes long, and spaced 1 or more weeks apart. (S) The accident scenario would result in a dose to a mximally exposed individual and to the...3.1-14 3.1.7 Grumman Corporation, Space and Electronics Division (U) 3.1-17 3.2 Ground Test Sites (U) 3.2-1 3.2.1 Nevada Test Site and Saddle Mountain

Structure and Dynamics Investigations of Sr/Ca-Doped LaPO 4 Proton Conductors

DOE Office of Scientific and Technical Information (OSTI.GOV)

al-Wahish, Amal; al-Binni, U.; Tetard, L.

Proton conductors loom out of the pool of candidate materials with great potential to boost hydrogen alternatives to fossil-based resources for energy. Acceptor doped lanthanum orthophosphates are considered for solid oxide fuel cells (SOFCs) for their potential stability and conductivity at high temperature. By exploring the crystal and defect structure of x% Sr/Ca-doped LaPO 4 with different nominal Sr/Ca concentrations (x = 0 – 10) with Neutron powder diffraction (NPD) and X-ray powder diffraction (XRD), we confirm that Sr/Ca-doped LaPO 4 can exist as self-supported structures at high temperatures during solid oxide fuel cell operation. Thermal stability, surface topography, sizemore » distribution are also studied to better understand the proton conductivity for dry and wet compounds obtained at sintering temperatures ranging from 1200 to 1400 °C using a combination of scanning electron microscopy (SEM), Atomic Force Microscopy (AFM), Fourier transform infrared spectroscopy (FTIR) and electrochemical impedance spectroscopy (EIS). In conclusion, the results suggest that Sr doped samples exhibit the highest proton conductivity of our samples and illustrate the impact of material design and versatile characterization schemes on the development of proton conductors with superior functionality.« less

76 FR 16444 - Eastern States: Filing of Plat of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-23

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLES956000-L14200000-BJ0000] Eastern States... described below in the BLM-Eastern States office in Springfield, Virginia. DATES: BLM will file the plat of survey on April 22, 2011. FOR FURTHER INFORMATION CONTACT: Bureau of Land Management-Eastern States, 7450...

75 FR 51840 - State of Arizona Resource Advisory Council Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-23

... Policy and Management Act of 1976 and the Federal Advisory Committee Act of 1972, the U.S. Department of... implemented; RAC questions on BLM District Managers' Reports; and reports by RAC working groups. A public...) Working Group Report, REA Working Group meeting schedule and future BLM/FS recreation fee proposals. The...

76 FR 55700 - Eastern States: Filing of Plat of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-08

...] Eastern States: Filing of Plat of Survey AGENCY: Bureau of Land Management, Interior. ACTION: Notice of Filing of Plat of Survey; Louisiana. SUMMARY: The Bureau of Land Management (BLM) will file the plat of survey of the lands described below in the BLM--Eastern States office in Springfield, Virginia, 30...

76 FR 45293 - Eastern States: Filing of Plat of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-28

...] Eastern States: Filing of Plat of Survey AGENCY: Bureau of Land Management, Interior. ACTION: Notice of Filing of Plat of Survey; Wisconsin. SUMMARY: The Bureau of Land Management (BLM) will file the plat of survey of the lands described below in the BLM-Eastern States office in Springfield, Virginia, 30...

76 FR 65533 - Eastern States: Filing of Plat of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-21

...] Eastern States: Filing of Plat of Survey AGENCY: Bureau of Land Management, Interior. ACTION: Notice of filing of plat of survey; North Carolina. SUMMARY: The Bureau of Land Management (BLM) will file the plat of survey of the land described below in the BLM-Eastern States office in Springfield, Virginia, 30...

76 FR 50492 - Idaho: Filing of Plats of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-15

... of Plats of Survey AGENCY: Bureau of Land Management, Interior. ACTION: Notice of filing of plats of surveys. SUMMARY: The Bureau of Land Management (BLM) has officially accepted the plat of survey of the... 83709-1657. SUPPLEMENTARY INFORMATION: The BLM will file the plat of survey of the lands described below...

75 FR 65028 - Eastern States: Filing of Plat of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-21

...] Eastern States: Filing of Plat of Survey AGENCY: Bureau of Land Management, Interior. ACTION: Notice of Filing of Plat of Survey; North Carolina. SUMMARY: The Bureau of Land Management (BLM) will file the plat of survey of the lands described below in the BLM-Eastern States office in Springfield, Virginia, 30...

75 FR 13302 - Eastern States: Filing of Plat of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

...] Eastern States: Filing of Plat of Survey AGENCY: Bureau of Land Management, Interior. ACTION: Notice of filing of plats of survey; North Carolina and Wisconsin. SUMMARY: The Bureau of Land Management (BLM) will file the plat of survey of the lands described below in the BLM-Eastern States office in...

77 FR 37919 - Eastern States: Filing of Plat of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-25

...: Filing of Plat of Survey AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: The Bureau of Land Management (BLM) will file the plat of survey of the lands described below in the BLM-Eastern..., Springfield, Virginia 22153. Attn: Cadastral Survey. Persons who use a telecommunications device for the deaf...

78 FR 16294 - Eastern States: Filing of Plat of Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-14

...: Filing of Plat of Survey AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: The Bureau of Land Management (BLM) will file the plat of survey of the lands described below in the BLM-Eastern..., Springfield, Virginia 22153. Attn: Cadastral Survey. Persons who use a telecommunications device for the deaf...