Sample records for element 104 258

  1. Observation of New Spontaneous Fission Activities from Elements 100 TO 105.

    NASA Astrophysics Data System (ADS)

    Somerville, Lawrence Patrick

    Several new Spontaneous Fission (SF) activities have been found. Their half-lives and production cross sections in several reactions have been measured by collecting and transporting recoils at known speed past mica track detectors. No definite identification could be made for any of the new SF activities; however, half-lives and possible assignments to element-104 isotopes consistent with several cross bombardments include ('257)Rf(3.8 s, 14% SF), ('258)Rf(13 ms), ('259)Rf((TURN)3 s, 8% SF), ('260)Rf((TURN)20 ms), and ('262)Rf((TURN)50ms). The 80-ms SF activity claimed by the Dubna group for the discovery of element 104 (('260)104) was not observed. A difficulty exists in the interpretation that ('260)Rf is a (TURN)20-ms SF activity: in order to be correct, for example, the SF activities with half-lives between 14 and 24 ms produced in the reactions 109- to 119-MeV ('18)O + ('248)Cm, 88- to 100-MeV ('15)N + ('249)Bk, and 96-MeV ('18)O + ('249)Cf must be other nuclides due to their large production cross sections, or the cross sections for production of ('260)Rf must be enhanced by unknown mechanisms. Based on calculated total production cross sections a possible (TURN)1% electron-capture branch in ('258)Lr(4.5 s) to the SF emitter ('258)No(1.2 ms) and an upper limit of 0.05% for SF branching in ('254)No(55 s) were determined. Other measured half-lives from unknown nuclides produced in respective reactions include (TURN)1.6 s (('18)O + ('248)Cm), indications of a (TURN)47-s SF activity (75-MeV ('12)C + ('249)Cf), and two or more SF activities with 3 s (LESSTHEQ) T(, 1/2) (LESSTHEQ) 60 s (('18)O + ('249)Bk). The most exciting conclusion of this work is that if the tentative assignments to even-even element -104 isotopes are correct, there would be a sudden change in the SF half-life systematics at element 104 which has been predicted theoretically by Randrup et al. and Baran et al. and attributed to the disappearance of the second hump of the double-humped fission barrier. This disappearance of the second barrier also explains the tentative low hindrance factors compared to lighter elements for SF of the odd -mass isotopes ('257)Rf((TURN)4 x 10('3)) and ('259)Rf((TURN)2 x 10('3)). On the basis of recent odd-mass alpha-decay energy data, the 152-neutron sub-shell effect is probably weaker for element 104 than for element 102, confirming predictions of Randrup et al., and not strong enough to significantly alter the SF half-life predictions. This weakening sub-shell effect is in contrast to the continuing strong effect assumed in the Ghiorso half-life systematics. The possibilities of enhanced stability against SF with 157 neutrons for ('261)RF(65 s) and theoretical arguments concerning the SF-mass distributions for element-104 nuclei are discussed.

  2. 76 FR 17859 - Notice of Public Information Collection(s) Being Reviewed by the Federal Communications...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-31

    ... required by the Paperwork Reduction Act (PRA) of 1995, 44 U.S.C. 3501-3520. Comments are requested... accuracy of the Commission's burden estimate; (c) ways to enhance the quality, utility, and clarity of the... is found at Sec. 258 [47 U.S.C. 258] Illegal Changes In Subscriber Carrier Selections, Public Law 104...

  3. A Versatile Model of Open-fracture Infection: a Contaminated Segmental Rat Femur Defect

    DTIC Science & Technology

    2014-06-01

    has the effect of introducing an implant to lower the bacte- rial inoculum required for infection.5 A 6 mm critical defect is then created in the mid...103 1 × 104 1 × 105 Bone mean 0 7.21 × 104 7.83 × 105 1.11 × 105 1.51 × 105 Bone SEM 0 4.40 × 104 1.35 × 105 1.92 × 104 1.95 × 104 Implant mean 0 9.82...104 1.65 × 105 2.58 × 104 5.11 × 104 Implant SEM 0 5.10 × 104 1.61 × 105 3.16 × 103 9.11 × 103 12 8 4 4 3 6 6 0 0 Pr op or tio n of s am p le s w

  4. High Velocity Jet Noise Source Location and Reduction. Task 6. Noise Abatement Nozzle Design Guide.

    DTIC Science & Technology

    1979-04-01

    the Conical Nozzle 255 on the Bertin Aerotrain . xvi ji4 ’ . _______ p .. LIST OF ILLUSTRATIONS (Continued) Figure Page D-37. Predicted and Measured...Moving-Frame Noise from the 256 Conical Nozzle on the Bertin Aerotrain . D-38. Predicted and Measured Static Noise from the 104-Tube 257 Nozzle on the...Bertin Aerotrain . D-39. Predicted and Measured Moving-Frame Noise from the 104- 258 Tube Nozzle on the Bertin Aerotrain . D-40. Relative Velocity Index m

  5. The −258 A/G (SNP rs12885300) polymorphism of the human type-2 deiodinase gene is associated with a shift in the pattern of secretion of thyroid hormones following a TRH-induced acute rise in TSH

    PubMed Central

    Peltsverger, Maya Y.; Butler, Peter W.; Alberobello, Anna Teresa; Smith, Sheila; Guevara, Yanina; Dubaz, Ornella M.; Luzon, Javier A.; Linderman, Joyce; Celi, Francesco S.

    2012-01-01

    Objective Type-2 deiodinase gene (DIO2) polymorphisms have been associated with changes in pituitary-thyroid axis homeostasis. The −258 A/G (SNP rs12885300) polymorphism has been associated with increased enzymatic activity, but data are conflicting. To characterize the effects of the −258 A/G polymorphism on intra-thyroidal T4 to T3 conversion and thyroid hormone secretion pattern we studied the effects of acute, TRH-mediated, TSH stimulation of the thyroid gland. Design Retrospective analysis. Methods The thyroid hormone secretion in response to 500 mcg iv TRH injection was studied in 45 healthy volunteers. Results Twenty-six subjects (16 females, 10 males, 32.8±10.4 years) were homozygous for the ancestral (−258 A/A) allele, 19 (11 females, 8 males, 31.1±10.9 years) were carrier of the (−258 G/x) variant. While no differences in the peak TSH and T3 levels were observed, carriers of the −258G/x allele showed a blunted rise in free T4 (p<0.01). The −258G/x 92Thr/Thr haplotype, compared to the other groups, had lower TSH values at 60' (p<0.03). No differences were observed between genotypes in baseline thyroid hormone levels. Conclusions The −258G/x DIO2 polymorphism variant is associated with a decreased rate of acute TSH-stimulated free T4 secretion with a normal T3 release from the thyroid consistent with a shift in the reaction equilibrium toward the product. These data indicate that the −258G DIO2 polymorphism cause changes in the pattern of hormonal secretion. These findings are a proof-of-concept that common polymorphisms in the DIO2 can subtly affect the circulating levels of thyroid hormone and might modulate the thyroid hormone homeostasis. PMID:22307573

  6. Genomic Analysis of the Emergence and Rapid Global Dissemination of the Clonal Group 258 Klebsiella pneumoniae Pandemic

    PubMed Central

    Driebe, Elizabeth M.; MacCannell, Duncan R.; Roe, Chandler; Lemmer, Darrin; de Man, Tom; Rasheed, J. Kamile; Engelthaler, David M.; Keim, Paul; Limbago, Brandi M.

    2015-01-01

    Multidrug-resistant Klebsiella pneumoniae producing the KPC carbapenemase have rapidly spread throughout the world, causing severe healthcare-associated infections with limited antimicrobial treatment options. Dissemination of KPC-producing K. pneumoniae is largely attributed to expansion of a single dominant strain, ST258. In this study, we explore phylogenetic relationships and evolution within ST258 and its clonal group, CG258, using whole genome sequence analysis of 167 isolates from 20 countries collected over 17 years. Our results show a common ST258 ancestor emerged from its diverse parental clonal group around 1995 and likely acquired bla KPC prior to dissemination. Over the past two decades, ST258 has remained highly clonal despite diversity in accessory elements and divergence in the capsule polysaccharide synthesis locus. Apart from the large recombination event that gave rise to ST258, few mutations set it apart from its clonal group. However, one mutation occurs in a global transcription regulator. Characterization of outer membrane protein sequences revealed a profile in ST258 that includes a truncated OmpK35 and modified OmpK37. Our work illuminates potential genomic contributors to the pathogenic success of ST258, helps us better understand the global dissemination of this strain, and identifies genetic markers unique to ST258. PMID:26196384

  7. Surveys of current status in biomedical science grant review: funding organisations' and grant reviewers' perspectives

    PubMed Central

    2010-01-01

    Background The objectives of this research were (a) to describe the current status of grant review for biomedical projects and programmes from the perspectives of international funding organisations and grant reviewers, and (b) to explore funders' interest in developing uniform requirements for grant review aimed at making the processes and practices of grant review more consistent, transparent, and user friendly. Methods A survey to a convenience sample of 57 international public and private organisations that give grants for biomedical research was conducted. Nine participating organisations then emailed a random sample of their external reviewers an invitation to participate in a second electronic survey. Results A total of 28 of 57 (49%) organisations in 19 countries responded. Organisations reported these problems as frequent or very frequent: declined review requests (16), late reports (10), administrative burden (7), difficulty finding new reviewers (4), and reviewers not following guidelines (4). The administrative burden of the process was reported to have increased over the past 5 years. In all, 17 organisations supported the idea of uniform requirements for conducting grant review and for formatting grant proposals. A total of 258/418 (62%) reviewers responded from 22 countries. Of those, 48% (123/258) said their institutions encouraged grant review, yet only 7% (17/258) were given protected time and 74% (192/258) received no academic recognition for this. Reviewers rated these factors as extremely or very important in deciding to review proposals: 51% (131/258) desire to support external fairness, 47% (120/258) professional duty, 46% (118/258) relevance of the proposal's topic, 43% (110/258) wanting to keep up to date, 40% (104/258) desire to avoid suppression of innovation. Only 16% (42/258) reported that guidance from funders was very clear. In all, 85% (220/258) had not been trained in grant review and 64% (166/258) wanted this. Conclusions Funders reported a growing workload of biomedical proposals that is getting harder to peer review. Just under half of grant reviewers take part for the good of science and professional development, but many report lack of academic and practical support and clear guidance. Around two-thirds of funders supported the development of uniform requirements for the format and peer review of proposals to help ease the current situation. PMID:20961441

  8. Method for Estimating Pesticide Use for County Areas of the Conterminous United States

    DTIC Science & Technology

    2000-01-01

    Insecticide 42,104 290,444 MCPB 1889 Herbicide 36,328 57,324 Metsulfuron 4003 Herbicide 35,534 7,747,924 Oxytetracycline 5038 Fungicide 35,321 71,207...22,841 114,203 Olives 22,441 6,397 Onions 16,110 76,704 Plums 15,092 30,005 Walnuts 12,552 46,490 Oxytetracycline Pears 17,638 45,258 Peaches 17,292

  9. A comparative study of burnout syndrome among health professionals in a Nigerian teaching hospital.

    PubMed

    Olley, B O

    2003-09-01

    Burnout as a measure of stress has generated research interest in the past two decades. However, there is a dearth of research on this interesting and important phenomenon in Nigeria. This study compared burnout and its associated factors in various health professionals working at the University College Hospital, Ibadan, Nigeria. Two hundred and sixty health care providers were sampled from 5 main units: Theatre/Intensive Care Unit (ICU), Accident and Emergency (A & E), Oncology, Dentistry and General Outpatients Department (GOP), among others. Included were 104 nurses (40%), 83 doctors (31.9%), 21 pharmacists/pharmacy technicians (8.0%), 10 medical social workers (3.8%) and 42 nursing assistants (16.1%). Outcome measures included the Maslach Burnout Inventory (MBI), the 30-item General Health Questionnaire (GHQ) and the Spielberger State Trait Anxiety Inventory (STAI). Core findings indicated that nurses consistently reported higher scores on all measures of burnout: exhaustion (F = 3.60, df = 258, P < .05); accomplishment (F = 3.94, df = 258, P < .05) and depersonalization (F = 4.58, df 258, P < .01) when compared with other health care providers. Significant differences were also noted between nurses and all other care providers in total scores on the General Health Questionnaire (F = 6.54, df 258, P < .01) and the State Trait Anxiety Inventory (F = 1.91, df 258, P < .05), respectively. These results are discussed in relation to the existing literature on burnout in Nigeria. Further empirical study is highly suggested in view of dearth of studies on the occupational health of health care providers in Nigeria.

  10. Pretreated Starch Solutions for Low Environmental Impact Aircraft Deicing

    DTIC Science & Technology

    2010-09-01

    modified monomeric organic molecules, have been the focus of recent study. Several organic acids and keto acids, such as succinate and acetate, have...1992), but the authors noted low economic feasibility without additional recovered products. Ganjyal et al. (2007) produced levulinate, a keto acid...534 -23.4 25 -22.7 52 569 -25.3 5 -25.8 104 585 -28.0 Sigma Aldrich DARCO 12-20 Mesh 0 -28.0 143 634 -28.0 50 -26.2 100 626 -27.4 Calgon F-300 9

  11. [Analysis of surface composition of three-way catalysts of in-use vehicles].

    PubMed

    Xie, Shu-xia; Hu, Jing-nan; Bao, Xiao-feng; Zhang, Ke-song; Li, Zhen-hua; Wang, Hai-tao

    2010-07-01

    The kinds and contents of surface elements in three-way catalysts of six light-duty in-use taxi cabs, which were mainly operated in Beijing and whose driving mileages were in the range of 34 x 10(4)-59 x 10(4) km, were determined by X-ray fluorescence spectrometry (XRF), and the effect of driving mileage on element content was investigated. Results showed that nearly 30 kinds of elements were present on the catalyst surface. The main elements of different samples were similar. The common elements of the pollutant on the front and rear catalysts were P, Ca, Zn and Mn etc., most of which are from engine oil and gasoline. S was only observed on the rear catalysts, indicating that S tends to deposit on the rear catalysts. After 34 x 10(4) km run, the P content increased very slowly and 40 x 10(4) km run S content reached a saturated value. While the contents of Ca, Zn and Mn still exhibit an increase tendency after 56 x 10(4) km. That means after 40 x 10(4) km driving mileage, the effects of P and S on the catalyst activity are minor, and the continuous deposit of Ca, Zn and Mn will lead to further decrease of the activity.

  12. L X-ray fluorescence cross sections of some rare earth elements ( Z = 62, 64, 66, 68 and 70) at 17.8, 22.6 and 25.8 keV

    NASA Astrophysics Data System (ADS)

    Mandal, A. C.; Santra, S.; Mitra, D.; Sarkar, M.; Bhattacharya, D.

    2005-06-01

    L X-ray fluorescence cross sections of the elements with Z = 62, 64, 66, 68 and 70 have been measured at 17.8, 22.6 and 25.8 keV using an X-ray tube and secondary exciters of Mo, Ag and Sn. The measured cross sections have been compared with the theoretical predictions and with the data of others. Theoretical values calculated using photoionisation cross sections from Scofield [Lawrence Livermore Laboratory, UCRL-51326, 1973], fluorescence yields and Coster-Kronig transition probabilities from Puri et al. [X-ray Spectrom. 22 (1993) 358] and radiative widths from Campbell and Wang [At. Data Nucl. Data Tables 43 (1989) 281] show good agreement with our data. Except two sets of data on Lγ cross sections, all the data of other groups agree well with those of ours.

  13. The effect of telephone reminders on attendance in respiratory outpatient clinics.

    PubMed

    Roberts, Nicola; Meade, Karen; Partridge, Martyn

    2007-04-01

    Patient non-attendance is an area of concern for all health care providers. A randomized controlled trial was undertaken to investigate whether reminder telephone calls improved attendance at respiratory outpatient clinics in the English National Health Service (NHS). Patients were randomly allocated into one of two groups, either telephone reminder group or usual care. The telephone reminder group received a reminder telephone call between 9 am and 5 pm during the week prior to their appointment. Attendance and demographic information (age, sex, diagnosis and home postcode) were recorded. A total of 504 patients were recruited, 258 patients were allocated to the control group and 246 patients were allocated to the telephone reminder group. Fifty-eight percent of the patients allocated to the telephone reminder group were not contactable. Within the telephone reminder group, of the 104 patients who could be contacted, 86% attended. There was a significant 15% increase in attendance in the contacted group (n = 104) when compared both with the control group (71%, n = 258) and with the patients who could not be contacted (68%, n = 142) (P = 0.007; P = 0.004). It was estimated that the cost of telephoning 200 patients could be offset by preventing one non-attendance. Routine telephoning of outpatients should become standard practice if reducing non-attendance is thought to be desirable, but general practitioner (GP) referral letters and hospital records of current hospital outpatients need to include an up-to-date telephone number. Consideration should be given to 'out-of-hours' reminder calls to maximize the contact rate.

  14. Synthesis of rutherfordium isotopes in the 238U(26Mg, xn)264-xRf reaction and study of their decay properties

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gates, Jacklyn M; Gates, J.M.; Garcia, M.A.

    2008-01-15

    Isotopes of rutherfordium (258-261Rf) were produced in irradiations of 238U targets with 26Mg beams. Excitation functions were measured for the 4n, 5n and 6n exit channels. Production of 261Rf in the 3n exit channel with a cross section of 28+92-26 pb was observed. Alpha decay of 258Rf was observed for the first time with an alpha-particle energy of 9.05+-0.03 MeV and an alpha/total decay branching ratio of 0.31+-0.11. In 259Rf, the electron capture/total decay branching ratio was measured to be 0.15+-0.04. The measured half-lives for 258Rf, 259Rf and 260Rf were 14.7+1.2-1.0 ms, 2.5+0.4-0.3 s and 22.2+3.0-2.4 ms, respectively, in agreementmore » with literature data. The systematics of the alpha decay Q values and of the partial spontaneous fission half-lives were evaluated for even-even nuclides in the region of the N = 152, Z = 100 deformed shell. The influence of the N = 152 shell on the alpha decay Q values for rutherfordium was observed to be similar to that of the lighter elements (96<_ Z<_ 102). However, the N = 152 shell does not stabilize the rutherfordium isotopes against spontaneous fission, as it does in the lighter elements (96<_ Z<_102).« less

  15. Gastrointestinal parasites of canids, a latent risk to human health in Tunisia.

    PubMed

    Oudni-M'rad, Myriam; Chaâbane-Banaoues, Raja; M'rad, Selim; Trifa, Fatma; Mezhoud, Habib; Babba, Hamouda

    2017-06-05

    Although data on the parasite environmental contamination are crucial to implement strategies for control and treatment, information about zoonotic helminths is very limited in Tunisia. Contamination of areas with canid faeces harboring infective parasite elements represents a relevant health-risk impact for humans. The aim of this study was to assess the environmental contamination with eggs and oocysts of gastrointestinal parasites of dogs and wild canids in Tunisia with special attention to those that can be transmitted to humans. One thousand two hundred and seventy faecal samples from stray dogs and 104 from wild canids (red foxes and golden jackals) were collected from different geographical regions throughout Tunisia. The helminth eggs and protozoan oocysts were concentrated by sucrose flotation and identified by microscopic examination. The most frequently observed parasites in dog samples were Toxocara spp. (27.2%), E. granulosus (25.8%), and Coccidia (13.1%). For wild canid faeces, the most commonly encountered parasites were Toxocara spp. (16.3%) followed by Capillaria spp. (9.6%). The parasite contamination of dog faeces varied significantly from one region to another in function of the climate. To our knowledge, the study highlights for the first time in Tunisia a serious environmental contamination by numerous parasitic stages infective to humans. Efforts should be made to increase the awareness of the contamination risk of such parasites in the environment and implement a targeted educational program.

  16. 40 CFR 258.17-258.19 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false [Reserved] 258.17-258.19 Section 258.17-258.19 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions §§ 258.17-258.19 [Reserved] ...

  17. 40 CFR 258.43-258.49 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false [Reserved] 258.43-258.49 Section 258.43-258.49 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Design Criteria §§ 258.43-258.49 [Reserved] ...

  18. 40 CFR 258.5-258.9 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false [Reserved] 258.5-258.9 Section 258.5-258.9 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General §§ 258.5-258.9 [Reserved] ...

  19. 40 CFR 258.30-258.39 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false [Reserved] 258.30-258.39 Section 258.30-258.39 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria §§ 258.30-258.39 [Reserved] ...

  20. 40 CFR 258.17-258.19 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false [Reserved] 258.17-258.19 Section 258.17-258.19 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions §§ 258.17-258.19 [Reserved] ...

  1. 40 CFR 258.5-258.9 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false [Reserved] 258.5-258.9 Section 258.5-258.9 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General §§ 258.5-258.9 [Reserved] ...

  2. 40 CFR 258.43-258.49 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false [Reserved] 258.43-258.49 Section 258.43-258.49 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Design Criteria §§ 258.43-258.49 [Reserved] ...

  3. 40 CFR 258.30-258.39 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false [Reserved] 258.30-258.39 Section 258.30-258.39 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria §§ 258.30-258.39 [Reserved] ...

  4. 40 CFR 258.62-258.69 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false [Reserved] 258.62-258.69 Section 258.62-258.69 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Closure and Post-Closure Care §§ 258.62-258.69 [Reserved] ...

  5. 40 CFR 258.63-258.69 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false [Reserved] 258.63-258.69 Section 258.63-258.69 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Closure and Post-Closure Care §§ 258.63-258.69 [Reserved] ...

  6. 48 CFR 9.104-1 - General standards.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Section 9.104-1 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING... them (including, as appropriate, such elements as production control procedures, property control... necessary production, construction, and technical equipment and facilities, or the ability to obtain them...

  7. 48 CFR 9.104-1 - General standards.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Section 9.104-1 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING... them (including, as appropriate, such elements as production control procedures, property control... necessary production, construction, and technical equipment and facilities, or the ability to obtain them...

  8. 48 CFR 9.104-1 - General standards.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Section 9.104-1 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING... them (including, as appropriate, such elements as production control procedures, property control... necessary production, construction, and technical equipment and facilities, or the ability to obtain them...

  9. [An analysis on funded theses in the Chinese Journal of Parasitology and Parasitic Diseases in 2009-2012].

    PubMed

    Yi, Feng-yun; Zhang, Zheng-yan; Sheng, Hui-feng

    2014-12-01

    The published articles of the Chinese Journal of Parasitology and Parasitic Diseases in 2009-2012 (including original articles, experimental researches, field researches and clinical researches) were statistically analyzed. Together 258 research papers were published in the 4 years, and funded papers occupied 82.2% (212/258). The number of papers funded by 1, 2, 3, 4, and 5 foundations projects was 116, 58, 29, 7 and 2, respectively. 61.8% (131/212) of the foundations projects were at the national level; 28.3% (60/212) were at provincial and ministerial level. The papers supported by academy and international agencies accounted for 7.1% (15/212), 2.8% (6/212), respectively. The funded thesis mainly referred to schistosomiasis (35, 16.5%), cystic echinococcosis (29, 13.7%), malaria (24, 11.3%), toxoplasmosis (22, 10.4%), and cysticercosis (9, 4.2%). Five fields covered in these papers were as follows: epidemiology (29, 13.7%), immunology and diagnosis (53, 25%), molecular biology (75, 35.4%), etiology (28,13.2%), and pharmacology (24, 11.3%). The ratio of founded paper was 0.70, 0.67, 0.74, and 0.65 during 2009-2012, respectively. The high ratio of founded paper indicated that this journal is with domestic and abroad importance in the field of parasitology.

  10. Ultrathin thermoacoustic nanobridge loudspeakers from ALD on polyimide

    NASA Astrophysics Data System (ADS)

    Brown, J. J.; Moore, N. C.; Supekar, O. D.; Gertsch, J. C.; Bright, V. M.

    2016-11-01

    The recent development of low-temperature (<200 °C) atomic layer deposition (ALD) for fabrication of freestanding nanostructures has enabled consideration of active device design based on engineered ultrathin films. This paper explores audible sound production from thermoacoustic loudspeakers fabricated from suspended tungsten nanobridges formed by ALD. Additionally, this paper develops an approach to lumped-element modeling for design of thermoacoustic nanodevices and relates the near-field plane wave model of individual transducer beams to the far-field spherical wave sound pressure that can be measured with standard experimental techniques. Arrays of suspended nanobridges with 25.8 nm thickness and sizes as small as 17 μm × 2 μm have been fabricated and demonstrated to produce audible sound using the thermoacoustic effect. The nanobridges were fabricated by ALD of 6.5 nm Al2O3 and 19.3 nm tungsten on sacrificial polyimide, with ALD performed at 130 °C and patterned by standard photolithography. The maximum observed loudspeaker sound pressure level (SPL) is 104 dB, measured at 20 kHz, 9.71 W input power, and 1 cm measurement distance, providing a loudspeaker sensitivity value of ∼64.6 dB SPL/1 mW. Sound production efficiency was measured to vary proportional to frequency f 3 and was directly proportional to input power. The devices in this paper demonstrate industrially feasible nanofabrication of thermoacoustic transducers and a sound production mechanism pertinent to submicron-scale device engineering.

  11. 104. Photocopied August 1978. CYLINDER USED IN THE ERECTION OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    104. Photocopied August 1978. CYLINDER USED IN THE ERECTION OF THE INCLINED BUTTRESSES FOR POWER HOUSE REINFORCEMENT IN 1916. AN AIR LOCK WAS PLACED ON TOP OF THE CYLINDER: THE LOWER PORTION OF THE VERTICAL ELEMENT RESTED ON THE POWER HOUSE FOUNDATION APRON: THE INCLINED ELEMENT WAS CUT LEVEL WITH THE RIVER BED. THE INCLINED PORTION OF THE CYLINDER CONTAINED THE SHIELD USED TO BEGIN THE ERECTION OF THE SEGMENTED INCLINED CAST IRON BUTTRESSES. (764) - Michigan Lake Superior Power Company, Portage Street, Sault Ste. Marie, Chippewa County, MI

  12. Lithographic microfabrication of biocompatible polymers for tissue engineering and lab-on-a-chip applications

    NASA Astrophysics Data System (ADS)

    Balciunas, Evaldas; Jonusauskas, Linas; Valuckas, Vytautas; Baltriukiene, Daiva; Bukelskiene, Virginija; Gadonas, Roaldas; Malinauskas, Mangirdas

    2012-06-01

    In this work, a combination of Direct Laser Writing (DLW), PoliDiMethylSiloxane (PDMS) soft lithography and UV lithography was used to create cm- scale microstructured polymer scaolds for cell culture experiments out of dierent biocompatible materials: novel hybrid organic-inorganic SZ2080, PDMS elastomer, biodegradable PEG- DA-258 and SU-8. Rabbit muscle-derived stem cells were seeded on the fabricated dierent periodicity scaolds to evaluate if the relief surface had any eect on cell proliferation. An array of microlenses was fabricated using DLW out of SZ2080 and replicated in PDMS and PEG-DA-258, showing good potential applicability of the used techniques in many other elds like micro- and nano- uidics, photonics, and MicroElectroMechanical Systems (MEMS). The synergetic employment of three dierent fabrication techniques allowed to produce desired objects with low cost, high throughput and precision as well as use materials that are dicult to process by other means (PDMS and PEG-DA-258). DLW is a relatively slow fabrication method, since the object has to be written point-by-point. By applying PDMS soft lithography, we were enabled to replicate laser-fabricated scaolds for stem cell growth and micro-optical elements for lab-on-a-chip applications with high speed, low cost and good reproducible quality.

  13. HSF and Msn2/4p can exclusively or cooperatively activate the yeast HSP104 gene.

    PubMed

    Grably, Melanie R; Stanhill, Ariel; Tell, Osnat; Engelberg, David

    2002-04-01

    In an effort to understand how an accurate level of stress-specific expression is obtained, we studied the promoter of the yeast HSP104 gene. Through 5' deletions, we defined a 334 bp fragment upstream of the first coding AUG as sufficient and essential for maximal basal activity and a 260 bp fragment as sufficient and essential for heat shock responsiveness. These sequences contain heat shock elements (HSEs) and stress response elements (STREs) that cooperate to achieve maximal inducible expression. However, in the absence of one set of factors (e.g. in msn2Deltamsn4Delta cells) proper induction is obtained exclusively through HSEs. We also show that HSP104 is constitutively derepressed in ras2Delta cells. This derepression is achieved exclusively through activation of STREs, with no role for HSEs. Strikingly, in ras2Deltamsn2Deltamsn4Delta cells the HSP104 promoter is also derepressed, but in this strain derepression is mediated through HSEs, showing the flexibility and adaptation of the promoter. Thus, appropriate transcription of HSP104 is usually obtained through cooperation between the Msn2/4/STRE and the HSF/ HSE systems, but each factor could activate the promoter alone, backing up the other. Transcription control of HSP104 is adaptive and robust, ensuring proper expression under extreme conditions and in various mutants.

  14. Information Needs Assessment for a Medicine Ward-Focused Rounding Dashboard.

    PubMed

    Aakre, Christopher A; Chaudhry, Rajeev; Pickering, Brian W; Herasevich, Vitaly

    2016-08-01

    To identify the routine information needs of inpatient clinicians on the general wards for the development of an electronic dashboard. Survey of internal medicine and subspecialty clinicians from March 2014-July 2014 at Saint Marys Hospital in Rochester, Minnesota. An information needs assessment was generated from all unique data elements extracted from all handoff and rounding tools used by clinicians in our ICUs and general wards. An electronic survey was distributed to 104 inpatient medical providers. 89 unique data elements were identified from currently utilized handoff and rounding instruments. All data elements were present in our multipurpose ICU-based dashboard. 42 of 104 (40 %) surveys were returned. Data elements important (50/89, 56 %) and unimportant (24/89, 27 %) for routine use were identified. No significant differences in data element ranking were observed between supervisory and nonsupervisory roles. The routine information needs of general ward clinicians are a subset of data elements used routinely by ICU clinicians. Our findings suggest an electronic dashboard could be adapted from the critical care setting to the general wards with minimal modification.

  15. The Impact of Complexity on the Expertise Reversal Effect: Experimental Evidence from Testing Accounting Students

    ERIC Educational Resources Information Center

    Blayney, Paul; Kalyuga, Slava; Sweller, John

    2016-01-01

    Element interactivity is a central concept of cognitive load theory that defines the complexity of a learning task. The reduction of task complexity through a temporary segmentation or isolation of interacting elements was investigated with 104 students randomly assigned to an interacting elements group, where participants were required to deal…

  16. Trace Elements Contamination and Human Health Risk Assessment in Drinking Water from the Agricultural and Pastoral Areas of Bay County, Xinjiang, China.

    PubMed

    Turdi, Muyessar; Yang, Linsheng

    2016-09-23

    Tap water samples were collected from 180 families in four agricultural (KYR: Keyir, KRW: Kariwak, YTR: Yatur, DW: Dawanqi) and two pastoral areas (B: Bulong and Y: Yangchang) in Bay County, Xinjiang, China, and levels of seven trace elements (Cd, Cr, As Ni, Pb, Zn, Se) were analyzed using inductively-coupled plasma mass spectrometry (ICP-MS) to assess potential health risks. Remarkable spatial variations of contamination were observed. Overall, the health risk was more severe for carcinogenic versus non-carcinogenic pollutants due to heavy metal. The risk index was greater for children overall (Cr > As > Cd and Zn > Se for carcinogenic and non-carcinogenic elements, respectively). The total risk index was greater in agricultural areas (DW > KYR > YTR > KRW > B > Y). Total risk indices were greater where well water was the source versus fountain water; for the latter, the total health risk index was greater versus glacier water. Main health risk factors were Cr and As in DW, KYR, YTR, KRW, and B, and Zn, Cr, and As in the Y region. Overall, total trace element-induced health risk (including for DW adults) was higher than acceptable (10(-6)) and lower than priority risk levels (10(-4)) (KYR, YTR, KRW, Y, and B). For DW children, total health risk reached 1.08 × 10(-4), higher than acceptable and priority risk levels (10(-4)).

  17. K→(ππ)(I=2) decay amplitude from lattice QCD.

    PubMed

    Blum, T; Boyle, P A; Christ, N H; Garron, N; Goode, E; Izubuchi, T; Jung, C; Kelly, C; Lehner, C; Lightman, M; Liu, Q; Lytle, A T; Mawhinney, R D; Sachrajda, C T; Soni, A; Sturm, C

    2012-04-06

    We report on the first realistic ab initio calculation of a hadronic weak decay, that of the amplitude A(2) for a kaon to decay into two π mesons with isospin 2. We find ReA(2)=(1.436±0.063(stat)±0.258(syst))10(-8) GeV in good agreement with the experimental result and for the hitherto unknown imaginary part we find ImA(2)=-(6.83±0.51(stat)±1.30(syst))10(-13) GeV. Moreover combining our result for ImA(2) with experimental values of ReA(2), ReA(0), and ε'/ε, we obtain the following value for the unknown ratio ImA(0)/ReA(0) within the standard model: ImA(0)/ReA(0)=-1.63(19)(stat)(20(syst)×10(-4). One consequence of these results is that the contribution from ImA(2) to the direct CP violation parameter ε' (the so-called Electroweak Penguin contribution) is Re(ε'/ε)(EWP)=-(6.52±0.49(stat)±1.24(syst))×10(-4). We explain why this calculation of A(2) represents a major milestone for lattice QCD and discuss the exciting prospects for a full quantitative understanding of CP violation in kaon decays. © 2012 American Physical Society

  18. Biodegradation of international jet A-1 aviation fuel by microorganisms isolated from aircraft tank and joint hydrant storage systems.

    PubMed

    Itah, A Y; Brooks, A A; Ogar, B O; Okure, A B

    2009-09-01

    Microorganisms contaminating international Jet A-1 aircraft fuel and fuel preserved in Joint Hydrant Storage Tank (JHST) were isolated, characterized and identified. The isolates were Bacillus subtillis, Bacillus megaterium, Flavobacterium oderatum, Sarcina flava, Micrococcus varians, Pseudomonas aeruginosa, Bacillus licheniformis, Bacillus cereus and Bacillus brevis. Others included Candida tropicalis, Candida albicans, Saccharomyces estuari, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus, Cladosporium resinae, Penicillium citrinum and Penicillium frequentans. The viable plate count of microorganisms in the Aircraft Tank ranged from 1.3 (+/-0.01) x 104 cfu/mL to 2.2 (+/-1.6) x 104 cfu/mL for bacteria and 102 cfu/mL to 1.68 (+/-0.32) x 103 cfu/mL for fungi. Total bacterial counts of 1.79 (+/-0.2) x 104 cfu/mL to 2.58 (+/-0.04) x 104 cfu/mL and total fungal count of 2.1 (+/-0.1) x 103 cfu/mL to 2.28 (+/-0.5) x 103 cfu/mL were obtained for JHST. Selected isolates were re-inoculated into filter sterilized aircraft fuels and biodegradation studies carried out. After 14 days incubation, Cladosporium resinae exhibited the highest degradation rate with a percentage weight loss of 66 followed by Candida albicans (60.6) while Penicillium citrinum was the least degrader with a weight loss of 41.6%. The ability of the isolates to utilize the fuel as their sole source of carbon and energy was examined and found to vary in growth profile between the isolates. The results imply that aviation fuel could be biodegraded by hydrocarbonoclastic microorganisms. To avert a possible deterioration of fuel quality during storage, fuel pipe clogging and failure, engine component damage, wing tank corrosion and aircraft disaster, efficient routine monitoring of aircraft fuel systems is advocated.

  19. A method for dye extraction using an aqueous two-phase system: Effect of co-occurrence of contaminants in textile industry wastewater.

    PubMed

    Borges, Gabriella Alexandre; Silva, Luciana Pereira; Penido, Jussara Alves; de Lemos, Leandro Rodrigues; Mageste, Aparecida Barbosa; Rodrigues, Guilherme Dias

    2016-12-01

    This paper reports a green and efficient procedure for extraction of the dyes Malachite Green (MG), Methylene Blue (MB), and Reactive Red 195 (RR) using an aqueous two-phase system (ATPS). An ATPS consists mainly of water, together with polymer and salt, and does not employ any organic solvent. The extraction efficiency was evaluated by means of the partition coefficients (K) and residual percentages (%R) of the dyes, under different experimental conditions, varying the tie-line length (TLL) of the system, the pH, the type of ATPS-forming electrolyte, and the type of ATPS-forming polymer. For MG, the best removal (K = 4.10 × 10(4), %R = 0.0069%) was obtained with the ATPS: PEO 1500 + Na2C4H4O6 (TLL = 50.21% (w/w), pH = 6.00). For MB, the maximum extraction (K = 559.9, %R = 0.258%) was achieved with the ATPS: PEO 400 + Na2SO4 (TLL = 50.31% (w/w), pH = 1.00). Finally for RR, the method that presented the best results (K = 3.75 × 10(4), %R = 0.237%) was the ATPS: PEO 400 + Na2SO4 (TLL = 50.31% (w/w), pH = 6.00). The method was applied to the recovery of these dyes from a textile effluent sample, resulting in values of K of 1.17 × 10(4), 724.1, and 3.98 × 10(4) for MG, MB, and RR, respectively, while the corresponding %R values were 0.0038, 0.154, and 0.023%, respectively. In addition, the ATPS methodology provided a high degree of color removal (96.5-97.95%) from the textile effluent. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Archaeological Investigations at Sites 45-OK-287 and 45-OK-288, Chief Joseph Dam Project, Washington.

    DTIC Science & Technology

    1984-01-01

    marks and evidence of burning by element ........ ........................ . 258 xviii .. . - ... ., ." xvii LIST OF PLATES Plate 1-1. 45-OK-287: view...which the site is located, and glaclotacustrlne sediments of two ages exposed In the opposite bank. The lowest major terrace the river cut In the...the site were exposed in fewer excavation units, but they have a finer grain size and greater proportion of angular cobbles, deriving from the bedrock

  1. 40 CFR 258.59 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false [Reserved] 258.59 Section 258.59 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.59 [Reserved] ...

  2. 40 CFR 258.52 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false [Reserved] 258.52 Section 258.52 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.52 [Reserved] ...

  3. 40 CFR 258.29 - Recordkeeping requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Recordkeeping requirements. 258.29 Section 258.29 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.29 Recordkeeping requirements. (a) The...

  4. 40 CFR 258.29 - Recordkeeping requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Recordkeeping requirements. 258.29 Section 258.29 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.29 Recordkeeping requirements. (a) The...

  5. 40 CFR 258.59 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false [Reserved] 258.59 Section 258.59 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.59 [Reserved] ...

  6. 40 CFR 258.52 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false [Reserved] 258.52 Section 258.52 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.52 [Reserved] ...

  7. Chemical studies of elements with Z ⩾ 104 in gas phase

    NASA Astrophysics Data System (ADS)

    Türler, Andreas; Eichler, Robert; Yakushev, Alexander

    2015-12-01

    Chemical investigations of superheavy elements in the gas-phase, i.e. elements with Z ≥ 104, allow assessing the influence of relativistic effects on their chemical properties. Furthermore, for some superheavy elements and their compounds quite unique gas-phase chemical properties were predicted. The experimental verification of these properties yields supporting evidence for a firm assignment of the atomic number. Prominent examples are the high volatility observed for HsO4 or the very weak interaction of Cn with gold surfaces. The unique properties of HsO4 were exploited to discover the doubly-magic even-even nucleus 270Hs and the new isotope 271Hs. The combination of kinematic pre-separation and gas-phase chemistry allowed gaining access to a new class of relatively fragile compounds, the carbonyl complexes of elements Sg through Mt. A not yet resolved issue concerns the interaction of Fl with gold surfaces. While competing experiments agree on the fact that Fl is a volatile element, there are discrepancies concerning its adsorption on gold surfaces with respect to its daughter Cn. The elucidation of these and other questions amounts to the fascination that gas-phase chemical investigations exert on current research at the extreme limits of chemistry today.

  8. Antibody-Mediated Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils

    PubMed Central

    Kobayashi, Scott D.; Porter, Adeline R.; Freedman, Brett; Pandey, Ruchi; Chen, Liang; Kreiswirth, Barry N.

    2018-01-01

    ABSTRACT Carbapenem-resistant Klebsiella pneumoniae is a problem worldwide. A carbapenem-resistant K. pneumoniae lineage classified as multilocus sequence type 258 (ST258) is prominent in the health care setting in many regions of the world, including the United States. ST258 strains can be resistant to virtually all clinically useful antibiotics; treatment of infections caused by these organisms is difficult, and mortality is high. As a step toward promoting development of new therapeutics for ST258 infections, we tested the ability of rabbit antibodies specific for ST258 capsule polysaccharide to enhance human serum bactericidal activity and promote phagocytosis and killing of these bacteria by human neutrophils. We first demonstrated that an isogenic wzy deletion strain is significantly more susceptible to killing by human heparinized blood, serum, and neutrophils than a wild-type ST258 strain. Consistent with the importance of capsule as an immune evasion molecule, rabbit immune serum and purified IgG specific for ST258 capsule polysaccharide type 2 (CPS2) enhanced killing by human blood and serum in vitro. Moreover, antibodies specific for CPS2 promoted phagocytosis and killing of ST258 by human neutrophils. Collectively, our findings suggest that ST258 CPS2 is a viable target for immunoprophylactics and/or therapeutics. PMID:29535199

  9. 40 CFR 258.27 - Surface water requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Surface water requirements. 258.27 Section 258.27 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.27 Surface water requirements. MSWLF...

  10. Performance of a PCR assay for the rapid identification of the Klebsiella pneumoniae ST258 epidemic clone in Latin American clinical isolates.

    PubMed

    Gomez, S A; Rapoport, M; Piergrossi, N; Faccone, D; Pasteran, F; De Belder, D; ReLAVRA-Group; Petroni, A; Corso, A

    2016-10-01

    The worldwide dissemination of Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae ST258 demands a rapid PCR-based typing method to detect unique genes of the ST258 clone. This study evaluates a PCR developed by Adler et al. (2014) for the detection of ST258 in K. pneumoniae clinical isolates centered on the identification of the pilv-I and prp genes. We tested 143 clinical isolates from Argentina (n=109), Chile (n=1), Colombia (n=1), Costa Rica (n=2), Ecuador (n=5), El Salvador (n=2), Nicaragua (n=5), Panamá (n=2), Paraguay (n=2), Perú (n=3) and Trinidad and Tobago (n=11) recovered from 2006 to 2015. blaKPC, pilv-l and prp genes were detected by PCR and sequenced by standard procedures. ST258 and non-ST258 were defined by PFGE and/or MLST. Isolates were grouped according to PFGE patterns: 58 were compatible with ST258 (group 1) and 85 with non-ST258 (group 2). MLST study was done on an arbitrary selection of isolates. The pilv-l gene was present only in ST258 isolates, regardless of the presence of the blaKPC gene. Results for the prp gene were variable. Its presence did not define ST258. The pilv-I PCR had a sensitivity and specificity of 100%, respectively, for the detection of ST258 in the isolates under investigation. Given our findings, the pilv-I PCR could replace more time and resource consuming methods, allowing for more rapid detection of the circulating high risk K. pneumoniae clone ST258 in Latin American (LA) countries. Copyright © 2016 Elsevier B.V. All rights reserved.

  11. Repulsion between Lys258 and upstream arginines explains the missorting of the AQP2 mutant p.Glu258Lys in nephrogenic diabetes insipidus.

    PubMed

    Kamsteeg, Erik-Jan; Stoffels, Monique; Tamma, Grazia; Konings, Irene B M; Deen, Peter M T

    2009-10-01

    Regulation of body water homeostasis occurs by the vasopressin-dependent sorting of aquaporin-2 (AQP2) water channels to and from the apical membrane of renal principal cells. Mutations in AQP2 cause autosomal nephrogenic diabetes insipidus (NDI), a disease that renders the kidney unresponsive to vasopressin, resulting in polyuria and polydipsia. The AQP2 mutant c.772G>A; p.Glu258Lys (AQP2-E258K) causes dominant NDI by oligomerizing with wild-type AQP2 and missorting of this AQP2 complex to multivesicular bodies instead of the apical membrane. The motif causing this missorting of AQP2-E258K was identified here. Functional analyses and plasma membrane expression studies of truncation mutants in oocytes revealed that AQP2-E258K shortened to Leu259 is still intracellular retained. Alanine scanning and glutamic acid to arginine exchanges revealed increased function and plasma membrane expression for AQP2-E258K mutants with the following additional changes: Leu259Ala, Arg252Glu, Arg253Glu, or Arg252Ala-Arg254Ala, or for the AQP2 mutant p.Glu258Ala, indicating that the motif RRRxxxK(258)L confers AQP2-E258K retention. Fusion of this motif to aquaporin-1 also resulted in missorting of that water channel, indicating that this retention motif is transferable. In conclusion, our data reveal that the RRRxxxKL motif and repulsion between K258 and the arginine-triplet within this motif are the primary cause of missorting of AQP2-E258K in NDI.

  12. 20 CFR 632.258 - Allowable activities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false Allowable activities. 632.258 Section 632.258 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INDIAN AND NATIVE AMERICAN EMPLOYMENT AND TRAINING PROGRAMS Summer Youth Employment and Training Programs § 632.258 Allowable activities...

  13. 20 CFR 632.258 - Allowable activities.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Allowable activities. 632.258 Section 632.258 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INDIAN AND NATIVE AMERICAN EMPLOYMENT AND TRAINING PROGRAMS Summer Youth Employment and Training Programs § 632.258 Allowable activities...

  14. 20 CFR 632.258 - Allowable activities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Allowable activities. 632.258 Section 632.258 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INDIAN AND NATIVE AMERICAN EMPLOYMENT AND TRAINING PROGRAMS Summer Youth Employment and Training Programs § 632.258 Allowable activities...

  15. 40 CFR 258.50 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Applicability. 258.50 Section 258.50 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL... and environment. (c) Owners and operators of MSWLF units, except those meeting the conditions of § 258...

  16. 40 CFR 258.10 - Airport safety.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Airport safety. 258.10 Section 258.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.10 Airport safety. (a) Owners or operators of new...

  17. 40 CFR 258.15 - Unstable areas.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Unstable areas. 258.15 Section 258.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.15 Unstable areas. (a) Owners or operators of new...

  18. 40 CFR 258.10 - Airport safety.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Airport safety. 258.10 Section 258.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.10 Airport safety. (a) Owners or operators of new...

  19. 40 CFR 258.15 - Unstable areas.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Unstable areas. 258.15 Section 258.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.15 Unstable areas. (a) Owners or operators of new...

  20. 40 CFR 258.56 - Assessment of corrective measures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Assessment of corrective measures. 258.56 Section 258.56 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.56...

  1. 40 CFR 258.70 - Applicability and effective date.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Applicability and effective date. 258.70 Section 258.70 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Financial Assurance Criteria § 258.70 Applicability and...

  2. 40 CFR 258.70 - Applicability and effective date.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Applicability and effective date. 258.70 Section 258.70 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Financial Assurance Criteria § 258.70 Applicability and...

  3. 46 CFR 107.258 - Crane certification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Crane certification. 107.258 Section 107.258 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.258 Crane certification. (a) The Coast Guard may accept current...

  4. 46 CFR 107.258 - Crane certification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Crane certification. 107.258 Section 107.258 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.258 Crane certification. (a) The Coast Guard may accept current...

  5. 46 CFR 107.258 - Crane certification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Crane certification. 107.258 Section 107.258 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.258 Crane certification. (a) The Coast Guard may accept current...

  6. 46 CFR 107.258 - Crane certification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Crane certification. 107.258 Section 107.258 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.258 Crane certification. (a) The Coast Guard may accept current...

  7. 46 CFR 107.258 - Crane certification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Crane certification. 107.258 Section 107.258 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.258 Crane certification. (a) The Coast Guard may accept current...

  8. 7 CFR 917.258 - Assessment rate.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Assessment rate. 917.258 Section 917.258 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... CALIFORNIA Assessment Rates § 917.258 Assessment rate. On and after March 1, 2010, an assessment rate of $0...

  9. 7 CFR 917.258 - Assessment rate.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Assessment rate. 917.258 Section 917.258 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... CALIFORNIA Assessment Rates § 917.258 Assessment rate. On and after March 1, 2009 an assessment rate of $0...

  10. 7 CFR 868.258 - Moisture.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 7 2013-01-01 2013-01-01 false Moisture. 868.258 Section 868.258 Agriculture... Governing Application of Standards § 868.258 Moisture. Water content in brown rice for processing as... purpose of this paragraph, “approved device” shall include the Motomco Moisture Meter and any other...

  11. 7 CFR 868.258 - Moisture.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 7 2012-01-01 2012-01-01 false Moisture. 868.258 Section 868.258 Agriculture... Governing Application of Standards § 868.258 Moisture. Water content in brown rice for processing as... purpose of this paragraph, “approved device” shall include the Motomco Moisture Meter and any other...

  12. 7 CFR 868.258 - Moisture.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false Moisture. 868.258 Section 868.258 Agriculture... Governing Application of Standards § 868.258 Moisture. Water content in brown rice for processing as... purpose of this paragraph, “approved device” shall include the Motomco Moisture Meter and any other...

  13. 6 CFR 25.8 - Government contractor Defense.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 6 Domestic Security 1 2012-01-01 2012-01-01 false Government contractor Defense. 25.8 Section 25.8...-TERRORISM BY FOSTERING EFFECTIVE TECHNOLOGIES § 25.8 Government contractor Defense. (a) Criteria for... applicability of the government contractor defense. In determining whether to issue such Certification, the...

  14. 6 CFR 25.8 - Government contractor Defense.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 6 Domestic Security 1 2013-01-01 2013-01-01 false Government contractor Defense. 25.8 Section 25.8...-TERRORISM BY FOSTERING EFFECTIVE TECHNOLOGIES § 25.8 Government contractor Defense. (a) Criteria for... applicability of the government contractor defense. In determining whether to issue such Certification, the...

  15. 40 CFR 258.57 - Selection of remedy.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 258.57 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.57 Selection of remedy... environment; and (4) Comply with standards for management of wastes as specified in § 258.58(d). (c) In...

  16. 40 CFR 258.57 - Selection of remedy.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 258.57 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.57 Selection of remedy... environment; and (4) Comply with standards for management of wastes as specified in § 258.58(d). (c) In...

  17. 40 CFR 258.14 - Seismic impact zones.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Seismic impact zones. 258.14 Section 258.14 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.14 Seismic impact zones. (a) New MSWLF units and...

  18. 40 CFR 258.14 - Seismic impact zones.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Seismic impact zones. 258.14 Section 258.14 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.14 Seismic impact zones. (a) New MSWLF units and...

  19. 42 CFR 84.258 - Fees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Fees. 84.258 Section 84.258 Public Health PUBLIC... RELATED ACTIVITIES APPROVAL OF RESPIRATORY PROTECTIVE DEVICES Special Use Respirators § 84.258 Fees. The following fees shall be charged for the examination, inspection, and testing of complete assemblies and...

  20. 6 CFR 25.8 - Government contractor Defense.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Government contractor Defense. 25.8 Section 25.8...-TERRORISM BY FOSTERING EFFECTIVE TECHNOLOGIES § 25.8 Government contractor Defense. (a) Criteria for... applicability of the government contractor defense. In determining whether to issue such Certification, the...

  1. 1. Photocopy of undated wash drawing. The largest building is ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Photocopy of undated wash drawing. The largest building is the Hotel Williams. Next to it is the Williams House (Williams Hotel Annex), HABS No. MI-258 A. Below them are the log cabins built by the American Fur Company (from left to right): Log Building No. 1 (MI-258 C), Log Building No. 2 (MI-258 D), Log Building No. 3 (not documented), Log Building No. 4 (MI-258 E), and Log Building No. 5 (MI-258 F). (Williams Log House, MI-258 B, and Log Building No. 8, MI-258 G, are not shown). The drawing is in the collection of the Michigan Historical Commission. Although it does not have a date, it was painted in the early 20th century after the main building was constructed. - Hotel Williams, Murray Bay, Munising, Alger County, MI

  2. Archaeological Investigations at Sites 45-OK-287 and 45-OK-288, Chief Joseph Dam Project, Washington.

    DTIC Science & Technology

    1984-01-01

    Table C-1. Distribution of butchering marks and evidence of burning by element .. ....... ....... ....... .. 258 xvii9. LIST OF PLATES Plate 1-1. 45-OK...the vicinity of the Omak Trench. Till forms the terrace on which the site Is located, and glaclolacustrIne sediments of two ! -ages exposed In the...the site were exposed In fewer excavation units, but they have a finer grain size and greater proportion of angular cobbles, deriving from the bedrock

  3. Operation Plan 14-44 ICEBERG

    DTIC Science & Technology

    1944-12-31

    report of de struction is required. C. H. McMOHKCS Chief of Staff 258 DISTRIBUTION : Same as Op- Plan 14-44 1. E. KEETOW Asst. Flag Secretary...w by author*/ oficLIss OPERATION PLAN 14—44 ICEBERG \\. CiNcPOA BRIG GEN W.A.DUMAS FORC6S UNCLAES1FO Report Documentation Page Form ApprovedOMB No...4. TITLE AND SUBTITLE Operation Plan 14-44 Iceberg 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT

  4. 38 CFR 3.258-3.259 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2011-07-01 2011-07-01 false [Reserved] 3.258-3.259 Section 3.258-3.259 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Pension, Compensation, and Dependency and Indemnity Compensation Dependency, Income and Estate §§ 3.258-3...

  5. 38 CFR 3.258-3.259 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false [Reserved] 3.258-3.259 Section 3.258-3.259 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Pension, Compensation, and Dependency and Indemnity Compensation Dependency, Income and Estate §§ 3.258-3...

  6. 38 CFR 3.258-3.259 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2013-07-01 2013-07-01 false [Reserved] 3.258-3.259 Section 3.258-3.259 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Pension, Compensation, and Dependency and Indemnity Compensation Dependency, Income and Estate §§ 3.258-3...

  7. 38 CFR 3.258-3.259 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2012-07-01 2012-07-01 false [Reserved] 3.258-3.259 Section 3.258-3.259 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Pension, Compensation, and Dependency and Indemnity Compensation Dependency, Income and Estate §§ 3.258-3...

  8. 38 CFR 3.258-3.259 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2014-07-01 2014-07-01 false [Reserved] 3.258-3.259 Section 3.258-3.259 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Pension, Compensation, and Dependency and Indemnity Compensation Dependency, Income and Estate §§ 3.258-3...

  9. 40 CFR 258.24 - Air criteria.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Air criteria. 258.24 Section 258.24... SOLID WASTE LANDFILLS Operating Criteria § 258.24 Air criteria. (a) Owners or operators of all MSWLFs... Implementation Plan (SIP) approved or promulgated by the Administrator pursuant to section 110 of the Clean Air...

  10. 40 CFR 258.24 - Air criteria.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Air criteria. 258.24 Section 258.24... SOLID WASTE LANDFILLS Operating Criteria § 258.24 Air criteria. (a) Owners or operators of all MSWLFs... Implementation Plan (SIP) approved or promulgated by the Administrator pursuant to section 110 of the Clean Air...

  11. 40 CFR 258.24 - Air criteria.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Air criteria. 258.24 Section 258.24... SOLID WASTE LANDFILLS Operating Criteria § 258.24 Air criteria. (a) Owners or operators of all MSWLFs... Implementation Plan (SIP) approved or promulgated by the Administrator pursuant to section 110 of the Clean Air...

  12. 40 CFR 258.73 - Financial assurance for corrective action.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... action. 258.73 Section 258.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID... the cost of hiring a third party to perform the corrective action in accordance with the program required under § 258.58 of this part. The corrective action cost estimate must account for the total costs...

  13. 40 CFR 258.75 - Discounting.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL... State may allow discounting of closure cost estimates in § 258.71(a), post-closure cost estimates in § 258.72(a), and/or corrective action costs in § 258.73(a) up to the rate of return for essentially risk...

  14. 40 CFR 258.73 - Financial assurance for corrective action.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... action. 258.73 Section 258.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID... the cost of hiring a third party to perform the corrective action in accordance with the program required under § 258.58 of this part. The corrective action cost estimate must account for the total costs...

  15. 40 CFR Appendix I to Part 258 - Constituents for Detection Monitoring

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Constituents for Detection Monitoring I Appendix I to Part 258 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Pt. 258, App. I Appendix I to Part 258—Constituents...

  16. 40 CFR Appendix I to Part 258 - Constituents for Detection Monitoring

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Constituents for Detection Monitoring I Appendix I to Part 258 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Pt. 258, App. I Appendix I to Part 258—Constituents...

  17. 40 CFR 258.3 - Consideration of other Federal laws.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... 258.3 Section 258.3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General § 258.3 Consideration of other Federal laws. The owner or operator of a municipal solid waste landfill unit must comply with any other applicable Federal...

  18. 40 CFR 258.3 - Consideration of other Federal laws.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... 258.3 Section 258.3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General § 258.3 Consideration of other Federal laws. The owner or operator of a municipal solid waste landfill unit must comply with any other applicable Federal...

  19. 40 CFR 258.27 - Surface water requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Surface water requirements. 258.27 Section 258.27 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.27 Surface water requirements. MSWLF units shall not: (a) Cause a discharge of...

  20. 40 CFR 258.51 - Ground-water monitoring systems.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Ground-water monitoring systems. 258.51 Section 258.51 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.51 Ground-water monitoring systems. (a) A...

  1. 40 CFR 258.72 - Financial assurance for post-closure care.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... care. 258.72 Section 258.72 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID... dollars, of the cost of hiring a third party to conduct post-closure care for the MSWLF unit in compliance with the post-closure plan developed under § 258.61 of this part. The post-closure cost estimate used...

  2. 40 CFR 258.26 - Run-on/run-off control systems.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Run-on/run-off control systems. 258.26 Section 258.26 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.26 Run-on/run-off control systems. (a...

  3. 40 CFR 258.26 - Run-on/run-off control systems.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Run-on/run-off control systems. 258.26 Section 258.26 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.26 Run-on/run-off control systems. (a...

  4. 40 CFR 258.20 - Procedures for excluding the receipt of hazardous waste.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... of hazardous waste. 258.20 Section 258.20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.20 Procedures..., regulated hazardous waste means a solid waste that is a hazardous waste, as defined in 40 CFR 261.3, that is...

  5. 40 CFR 258.20 - Procedures for excluding the receipt of hazardous waste.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of hazardous waste. 258.20 Section 258.20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.20 Procedures..., regulated hazardous waste means a solid waste that is a hazardous waste, as defined in 40 CFR 261.3, that is...

  6. 40 CFR 258.53 - Ground-water sampling and analysis requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Ground-water sampling and analysis requirements. 258.53 Section 258.53 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.53 Ground-water sampling and analysi...

  7. 40 CFR 258.53 - Ground-water sampling and analysis requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Ground-water sampling and analysis requirements. 258.53 Section 258.53 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.53 Ground-water sampling and analysi...

  8. 40 CFR 258.10 - Airport safety.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Airport safety. 258.10 Section 258.10... SOLID WASTE LANDFILLS Location Restrictions § 258.10 Airport safety. (a) Owners or operators of new... meters) of any airport runway end used by turbojet aircraft or within 5,000 feet (1,524 meters) of any...

  9. 40 CFR 258.10 - Airport safety.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Airport safety. 258.10 Section 258.10... SOLID WASTE LANDFILLS Location Restrictions § 258.10 Airport safety. (a) Owners or operators of new... meters) of any airport runway end used by turbojet aircraft or within 5,000 feet (1,524 meters) of any...

  10. 40 CFR 258.10 - Airport safety.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Airport safety. 258.10 Section 258.10... SOLID WASTE LANDFILLS Location Restrictions § 258.10 Airport safety. (a) Owners or operators of new... meters) of any airport runway end used by turbojet aircraft or within 5,000 feet (1,524 meters) of any...

  11. 37 CFR 258.3 - Royalty fee for secondary transmission of analog signals of broadcast stations by satellite...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... transmission of analog signals of broadcast stations by satellite carriers. 258.3 Section 258.3 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES ADJUSTMENT OF ROYALTY FEE FOR SECONDARY TRANSMISSIONS BY SATELLITE CARRIERS § 258.3...

  12. 29 CFR 779.258 - Sales made or business done.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Sales made or business done. 779.258 Section 779.258 Labor... Volume of Sales Made Or Business Done § 779.258 Sales made or business done. The Senate Report on the 1966 amendments reaffirmed the intent to measure the “dollar volume of sales or business” including...

  13. 47 CFR 1.258 - Closing of the hearing.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Closing of the hearing. 1.258 Section 1.258... Intermediate Decision § 1.258 Closing of the hearing. The record of hearing shall be closed by an announcement... concluded. In the discretion of the presiding officer, the record may be closed as of a future specified...

  14. Application of screened Coulomb potential in fitting DBV star PG 0112+104

    NASA Astrophysics Data System (ADS)

    Chen, Y. H.

    2018-03-01

    With 78.7 d of observations for PG 0112+104, a pulsating DB star, from Campaign 8 of Kepler 2 mission, Hermes et al. made a detailed mode identification. A reliable mode identification, with 5 l = 1 modes, 3 l = 2 modes, and 3 l = 1 or 2 modes, was identified. Grids of DBV star models are evolved by WDEC with element diffusion effect of pure Coulomb potential and screened Coulomb potential. Fitting the identified modes of PG 0112+104 by the calculated ones, we studied the difference of element diffusion effect between adopting pure Coulomb potential and screened Coulomb potential. Our aim is to reduce the fitting error by studying new input physics. The starting models including their chemical composition profile are from white dwarf models evolved by MESA. They were calculated following the stellar evolution from the main sequence to the start of the white dwarf cooling sequences. The optimal parameters are basically consistent with that of previous spectroscopic and asteroseismological studies. The pure and screened Coulomb potential lead to different composition profiles of the C/O-He interface area. High k modes are very sensitive to the area. However, most of the observed modes for PG 0112+104 are low k modes. The σRMS taking the screened Coulomb potential is reduced by 4 per cent compared with taking the pure Coulomb potential when fitting the identified low k modes of PG 0112+104. Fitting the Kepler 2 data with our models improved the σRMS of the fit by 27 per cent.

  15. Burst Testing and Analysis of Superalloy Disks With a Dual Grain Microstructure

    NASA Technical Reports Server (NTRS)

    Gayda, John; Kantzos, Pete

    2006-01-01

    Elastic-plastic finite element analyses of room temperature burst tests on four superalloy disks were conducted and reported in this paper. Two alloys, Rene 104 (General Electric Aircraft Engines) and Alloy 10 (Honeywell Engines & Systems), were studied. For both alloys an advanced dual microstructure disk, fine grain bore and coarse grain rim, were analyzed and compared with conventional disks with uniform microstructures, coarse grain for Rene 104 and fine grain for Alloy 10. The analysis and experimental data were in good agreement up to burst. At burst, the analysis underestimated the speed and growth of the Rene 104 disks, but overestimated the speed and growth of the Alloy 10 disks. Fractography revealed that the Alloy 10 disks displayed significant surface microcracking and coalescence in comparison to Rene 104 disks. This phenomenon may help explain the differences between the Alloy 10 disks and the Rene 104 disks, as well as the observed deviations between analytical and experimental data at burst.

  16. Evaluation of ADINA. Part I. Theory and Programing Descriptions.

    DTIC Science & Technology

    1980-06-08

    Problem," Numerical and Computer Methods in Structural Mechanics, S. J. Feaves, N. Pe-rone, J. Robinson and W.C. Schnobrich, eds., Academic Press, New...connectivity array N102 ’NDM*NlJME-ITW0 YL Element nodal coordinates N103 ---- NUME IELT Element number of nodes N104 NUME IPST Stress printing flag N105 NUME

  17. Control of microstructure and mechanical properties of laser solid formed Inconel 718 superalloy by electromagnetic stirring

    NASA Astrophysics Data System (ADS)

    Liu, Fencheng; Cheng, Hongmao; Yu, Xiaobin; Yang, Guang; Huang, Chunping; Lin, Xin; Chen, Jing

    2018-02-01

    The coarse columnar grains and special interface in laser solid formed (LSFed) Inconel 718 superalloy workpieces seriously affect their mechanical properties. To improve the microstructure and mechanical properties of LSFed Inconel 718 superalloy, electromagnetic stirring (EMS) was introduced to alter the solidification process of the molten pool during LSF. The results show that EMS could not completely eliminate the epitaxially growing columnar grains, however, the strong convection of liquid metals can effectively influence the solid-liquid interface growing mode. The segregation of alloying elements on the front of solid-liquid interface is inhibited and the degree of constitutional supercooling decreases correspondingly. Comparing the microstructures of samples formed under different process parameters, the size and amount of the γ+Laves eutectic phases formed in interdendritic area decrease along with the increasing magnetic field intensity, resulting in more uniformly distributed alloying elements. The residual stress distribution is proved to be more uniform, which is beneficial to the grain refinement after recrystallilzaiton. Mechanical properties testing results show an improvement of 100 MPa in tensile strength and 22% in elongation was obtained after EMS was used. The high cycle fatigue properties at room temperature was also improved from 4.09 × 104 cycles to 8.21 × 104 cycles for the as-deposited samples, and from 5.45 × 104 cycles to 12.73 × 104 cycles for the heat treated samples respectively.

  18. Exercise Ameliorates Endocrine Pancreas Damage Induced by Chronic Cola Drinking in Rats.

    PubMed

    Otero-Losada, Matilde; González, Julián; Müller, Angélica; Ottaviano, Graciela; Cao, Gabriel; Azzato, Francisco; Ambrosio, Giuseppe; Milei, José

    2016-01-01

    This study evaluates whether the daily practice of an exercise routine might protect from endocrine pancreas damage in cola drinking rats. Forty-eight Wistar rats were randomly assigned to 4 groups depending on a) beverage consumption ad libitum, water (W) or cola beverage (C), and b) physical activity, sedentary (S) or treadmill running (R). Accordingly, 4 groups were studied: WS (water sedentary), WR (water runner), CS (cola sedentary) and CR (cola runner). Body weight, nutritional data, plasma levels of glucose, creatinine, total cholesterol and cholesterol fractions, and triglycerides (enzymocolorimetry), and systolic blood pressure (plethysmography) were measured. After 6 months, euthanasia was performed (overdose sodium thiopental). Pancreatic tissue was immediately excised and conventionally processed for morphometrical and immunohistochemical determinations. The effects of running and chronic cola drinking on pancreas morphology showed interaction (p<0.001) rather than simple summation. Cola drinking (CS vs WS) reduced median pancreatic islet area (-30%, 1.8 104 μm2 vs 2.58 104 μm2, p<0.0001) and median β-cell mass (-43%, 3.81 mg vs 6.73 mg, p<0.0001), and increased median α/β ratio (+49%, 0.64 vs 0.43, p< 0.001). In water drinking rats (WR vs WS), running reduced median α-cell mass (-48%, 1.48 mg vs 2.82 mg, p<0.001) and α/β ratio (-56%, 0.19 vs 0.43, p<0.0001). Differently, in cola drinking rats (CR vs CS), running partially restored median islet area (+15%, 2.06 104 μm2 vs 1.79 104 μm2, p<0.05), increased median β-cell mass (+47%, 5.59 mg vs 3.81 mg, p <0.0001) and reduced median α/β ratio (-6%, 0.60 vs 0.64, p<0.05). This study is likely the first reporting experimental evidence of the beneficial effect of exercise on pancreatic morphology in cola-drinking rats. Presently, the increase of nearly 50% in β cells mass by running in cola drinking rats is by far the most relevant finding. Moderate running, advisably indicated in cola consumers and patients at risk of diabetes, finds here experimental support.

  19. Exercise Ameliorates Endocrine Pancreas Damage Induced by Chronic Cola Drinking in Rats

    PubMed Central

    Otero-Losada, Matilde; González, Julián; Müller, Angélica; Ottaviano, Graciela; Cao, Gabriel; Azzato, Francisco; Ambrosio, Giuseppe; Milei, José

    2016-01-01

    Purpose This study evaluates whether the daily practice of an exercise routine might protect from endocrine pancreas damage in cola drinking rats. Methods Forty-eight Wistar rats were randomly assigned to 4 groups depending on a) beverage consumption ad libitum, water (W) or cola beverage (C), and b) physical activity, sedentary (S) or treadmill running (R). Accordingly, 4 groups were studied: WS (water sedentary), WR (water runner), CS (cola sedentary) and CR (cola runner). Body weight, nutritional data, plasma levels of glucose, creatinine, total cholesterol and cholesterol fractions, and triglycerides (enzymocolorimetry), and systolic blood pressure (plethysmography) were measured. After 6 months, euthanasia was performed (overdose sodium thiopental). Pancreatic tissue was immediately excised and conventionally processed for morphometrical and immunohistochemical determinations. Results The effects of running and chronic cola drinking on pancreas morphology showed interaction (p<0.001) rather than simple summation. Cola drinking (CS vs WS) reduced median pancreatic islet area (-30%, 1.8 104 μm2 vs 2.58 104 μm2, p<0.0001) and median β-cell mass (-43%, 3.81 mg vs 6.73 mg, p<0.0001), and increased median α/β ratio (+49%, 0.64 vs 0.43, p< 0.001). In water drinking rats (WR vs WS), running reduced median α-cell mass (-48%, 1.48 mg vs 2.82 mg, p<0.001) and α/β ratio (-56%, 0.19 vs 0.43, p<0.0001). Differently, in cola drinking rats (CR vs CS), running partially restored median islet area (+15%, 2.06 104 μm2 vs 1.79 104 μm2, p<0.05), increased median β-cell mass (+47%, 5.59 mg vs 3.81 mg, p <0.0001) and reduced median α/β ratio (-6%, 0.60 vs 0.64, p<0.05). Conclusion This study is likely the first reporting experimental evidence of the beneficial effect of exercise on pancreatic morphology in cola-drinking rats. Presently, the increase of nearly 50% in β cells mass by running in cola drinking rats is by far the most relevant finding. Moderate running, advisably indicated in cola consumers and patients at risk of diabetes, finds here experimental support. PMID:27192084

  20. 40 CFR 258.29 - Recordkeeping requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 258.29 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.29 Recordkeeping requirements. (a) The... of an approved state program may receive electronic documents only if the state program includes the...

  1. 40 CFR 258.29 - Recordkeeping requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 258.29 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.29 Recordkeeping requirements. (a) The... of an approved state program may receive electronic documents only if the state program includes the...

  2. 40 CFR 258.29 - Recordkeeping requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 258.29 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.29 Recordkeeping requirements. (a) The... of an approved state program may receive electronic documents only if the state program includes the...

  3. 40 CFR 258.11 - Floodplains.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Floodplains. 258.11 Section 258.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL...: (1) Floodplain means the lowland and relatively flat areas adjoining inland and coastal waters...

  4. 40 CFR 258.11 - Floodplains.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Floodplains. 258.11 Section 258.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL...: (1) Floodplain means the lowland and relatively flat areas adjoining inland and coastal waters...

  5. 40 CFR 258.11 - Floodplains.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Floodplains. 258.11 Section 258.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL...: (1) Floodplain means the lowland and relatively flat areas adjoining inland and coastal waters...

  6. 40 CFR 258.11 - Floodplains.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Floodplains. 258.11 Section 258.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL...: (1) Floodplain means the lowland and relatively flat areas adjoining inland and coastal waters...

  7. 40 CFR 258.11 - Floodplains.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Floodplains. 258.11 Section 258.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL...: (1) Floodplain means the lowland and relatively flat areas adjoining inland and coastal waters...

  8. 37 CFR 258.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Definitions. 258.2 Section 258.2 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES ADJUSTMENT OF ROYALTY FEE FOR SECONDARY TRANSMISSIONS BY SATELLITE...

  9. 37 CFR 258.1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES ADJUSTMENT OF ROYALTY FEE FOR SECONDARY TRANSMISSIONS BY SATELLITE CARRIERS § 258.1 General. This part 258 adjusts the rates of royalties payable under the compulsory license for...

  10. 40 CFR 258.28 - Liquids restrictions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 258.28 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.28 Liquids restrictions. (a) Bulk or... (Paint Filter Liquids Test), included in “Test Methods for Evaluating Solid Waste, Physical/Chemical...

  11. 40 CFR 258.28 - Liquids restrictions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 258.28 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.28 Liquids restrictions. (a) Bulk or... (Paint Filter Liquids Test), included in “Test Methods for Evaluating Solid Waste, Physical/Chemical...

  12. Scanning Electron Microscopy-Energy-Dispersive X-Ray (SEM/EDX): A Rapid Diagnostic Tool to Aid the Identification of Burnt Bone and Contested Cremains.

    PubMed

    Ellingham, Sarah T D; Thompson, Tim J U; Islam, Meez

    2018-03-01

    This study investigates the use of Scanning electron microscopy-energy-dispersive X-ray (SEM-EDX) as a diagnostic tool for the determination of the osseous origin of samples subjected to different temperatures. Sheep (Ovis aries) ribs of two experimental groups (fleshed and defleshed) were burned at temperatures of between 100°C and 1100°C in 100°C increments and subsequently analyzed with the SEM-EDX to determine the atomic percentage of present elements. Three-factor ANOVA analysis showed that neither the exposure temperature, nor whether the burning occurred with or without soft tissue present had any significant influence on the bone's overall elemental makeup (p > 0.05). The Ca/P ratio remained in the osseous typical range of between 1.6 and 2.58 in all analyzed samples. This demonstrates that even faced with high temperatures, the overall gross elemental content and atomic percentage of elements in bone remain stable, creating a unique "fingerprint" for osseous material, even after exposure to extreme conditions. © 2017 American Academy of Forensic Sciences.

  13. Interference removals on Pd, Ru and Au with ICP-QQQ-MS in PGE RM

    NASA Astrophysics Data System (ADS)

    Nadeem Hussain Bokhari, Syed; Meisel, Thomas; Walkner, Christoph

    2015-04-01

    Gold and platinum group elements (PGE) are essential industrial precious metals with high world demand due to their unique properties. Struggle for natural exploration of PGE is on great pace and recycling from industrial wastes, electronics and catalytic convertor is on the rise for PGE supply chain. Along with these developments it is becoming more challenging for analytical chemists to determine gold and PGE out of complex matrix which causes severe interferences. The current state of art is online analysis coupled with chromatographic separation of interferences. The ICP-QQQ-MS Agilent 8800 has the capability of using multi tunes and mass shifts. We aim to remove interferences on Pd+ (for direct and isotope dilution analysis) Au+ and Ru+ in lieu of chemical separations. YO+, SrOH+, ZnAr+, NiAr+, ZrO+, CuAr+, MoO+ , Ru+and Cd+ are expected interferences on Pd+ while Au+ is interfered by TaO+, HfOH+, GdAr+ and 102Ru+ ,104Ru+ by 102Pd+ ,104Pd+ etc. Initial test were performed on pure solutions of 1mg/l (interfering elements): 1 ng/l (Pd, Ru & Au) respectively. The outcomes of initial tests were applied on PGE reference material (RM) WMG-1 and SARM-7 (digested with Na2O2 sintering). The results obtained show that YO+, SrOH+ interfere (104Pd,105Pd), 104 Ru+ on (104Pd), ZnAr+ has slight interference on (104Pd and106Pd), ZrO+, NiAr+, CuAr+ interferences are negligible, MoO+ has severe interference on (108Pd, 110Pd) and that Cd+ has severe isobaric interference on (106Pd,108Pd, 110Pd). These interference have been removed by formation of Pd(NH3)3+complex. The TaO+, HfOH+ and GdAr+ interferences on Au+ are best removed by formation of Au(NH3)+ and Au(NH3)2+ complexes. 102Pd+,104Pd+interference on 102Ru+ ,104Ru+ can be removed by formation of Ru(NH3)4+ and RuO+ compounds. The results obtained comply with certified values of RM. The developed method is being tested on low concentration PGE reference materials. References: Sugiyama, N. " Removal of complex spectral interferences on noble metal isotopes." Agilent 8800 ICP-QQQ Application Handbook, 2014, 42-46.

  14. 40 CFR 258.56 - Assessment of corrective measures.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Assessment of corrective measures. 258.56 Section 258.56 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... implementation, and potential impacts of appropriate potential remedies, including safety impacts, cross-media...

  15. 40 CFR 258.25 - Access requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... wastes by using artificial barriers, natural barriers, or both, as appropriate to protect human health... 258.25 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.25 Access requirements. Owners or operators of all...

  16. 40 CFR 258.25 - Access requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... wastes by using artificial barriers, natural barriers, or both, as appropriate to protect human health... 258.25 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.25 Access requirements. Owners or operators of all...

  17. 40 CFR 258.25 - Access requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 258.25 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.25 Access requirements. Owners or operators of all... wastes by using artificial barriers, natural barriers, or both, as appropriate to protect human health...

  18. 40 CFR 258.25 - Access requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 258.25 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.25 Access requirements. Owners or operators of all... wastes by using artificial barriers, natural barriers, or both, as appropriate to protect human health...

  19. 14 CFR 258.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Purpose. 258.1 Section 258.1 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... consumers are adequately informed before they book air transportation or embark on travel involving change...

  20. 14 CFR 258.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Purpose. 258.1 Section 258.1 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... consumers are adequately informed before they book air transportation or embark on travel involving change...

  1. 14 CFR 258.1 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Purpose. 258.1 Section 258.1 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... consumers are adequately informed before they book air transportation or embark on travel involving change...

  2. 14 CFR 258.1 - Purpose.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Purpose. 258.1 Section 258.1 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... consumers are adequately informed before they book air transportation or embark on travel involving change...

  3. 14 CFR 258.1 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Purpose. 258.1 Section 258.1 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... consumers are adequately informed before they book air transportation or embark on travel involving change...

  4. 37 CFR 258.1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2011-07-01 2011-07-01 false General. 258.1 Section 258.1 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES ADJUSTMENT OF ROYALTY FEE FOR SECONDARY TRANSMISSIONS BY SATELLITE CARRIERS...

  5. Violence and weapon-related trauma at Puruchuco-Huaquerones, Peru.

    PubMed

    Murphy, Melissa S; Gaither, Catherine; Goycochea, Elena; Verano, John W; Cock, Guillermo

    2010-08-01

    Conquest of indigenous peoples in North America is understood primarily through ethnohistorical documents, archaeological evidence, and osteological analyses. However, in the Central Andes, the colonial enterprise and its effects are understood only from postcontact historical and ethnohistorical sources. Few archaeological and bioarchaeological studies have investigated Spanish Conquest and colonialism in the Andean region [for exceptions see Klaus and Tam: Am J Phys Anthropol 138 (2009) 356-368; Wernke, in press; and Quilter, in press]. Here we describe bioarchaeological evidence of violence from the cemeteries of Huaquerones and 57AS03 within the archaeological zone of Puruchuco-Huaquerones, Peru (circa A.D. 1470-1540). A total of 258 individuals greater than 15 years of age were analyzed for evidence of traumatic injuries. Individuals were examined macroscopically and evidence of traumatic injuries was analyzed according to the skeletal element involved, the location of the injury on the skeletal element, and any additional complications of the injury. This study examines and compares the evidence of perimortem injuries on skeletonized individuals from the two cemeteries and focuses specifically on the interpretation of weapon-related perimortem injuries. Evidence of perimortem trauma is present in both cemeteries (18.6%, 48/258); however, the frequency of injuries in 57AS03 is greater than that in Huaquerones (25.0% vs. 13.0%). Several injuries from 57AS03 are consistent with documented cases of injuries from firearms and 16th Century European weapons. We believe that the nature and high frequency of perimortem trauma at 57AS03 provide evidence of the violence that occurred with Spanish Conquest of the Inca Empire. Copyright 2010 Wiley-Liss, Inc.

  6. Antimicrobial activity and mechanism of action of a novel cationic α-helical octadecapeptide derived from heat shock protein 70 of rice.

    PubMed

    Taniguchi, Masayuki; Ikeda, Atsuo; Nakamichi, Shun-Ichi; Ishiyama, Yohei; Saitoh, Eiichi; Kato, Tetsuo; Ochiai, Akihito; Tanaka, Takaaki

    2013-10-01

    Hsp70(241-258), an octadecapeptide derived from the heat shock protein 70 (Hsp70) of rice (Oryza sativa L. japonica), is a novel cationic α-helical antimicrobial peptide (AMP) that contains four lysine, two arginine, and two histidine residues. The antimicrobial activity of Hsp70(241-258) against Porphyromonas gingivalis, a periodontal pathogen, and Candida albicans, an opportunistic fungal pathogen, was quantitatively evaluated using a chemiluminescence method that measures ATP derived from viable cells. The 50% growth-inhibitory concentrations of Hsp70(241-258) against P. gingivalis and C. albicans cells were 63 μM and 70 μM, respectively. Hsp70(241-258) had little or no hemolytic activity even at 1mM, and showed negligible cytotoxicity up to 300 μM. The degrees of calcein leakage from large unilamellar vesicles, which mimic the membranes of Gram-negative bacteria, and 3,3'-dipropylthiadicarbocyanine iodide release from P. gingivalis cells induced by the addition of Hsp70(241-258) increased in a concentration-dependent manner. When Hsp70(241-258) was added to calcein-acetoxymethyl ester-loaded C. albicans cells, calcein release from the cells increased in a concentration-dependent manner. Flow cytometric analysis also showed that the percentages of C. albicans cells stained with propidium iodide, a DNA-intercalating dye, increased as the concentration of Hsp70(241-258) added was increased. Therefore, Hsp70(241-258) appears to exhibit antimicrobial activity against P. gingivalis and C. albicans through membrane disruption. These results suggest that Hsp70(241-258) could be useful as a safe and potent AMP against P. gingivalis and C. albicans in many fields of health care, especially in the control of oral infections. Copyright © 2013 Elsevier Inc. All rights reserved.

  7. 36 CFR 13.104 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., completely enclosed, with a roof and walls which may have windows and door(s). Claimant means a person who.... Shelter means a structure designed to provide temporary relief from the elements and is characterized as a...

  8. Hsf1p and Msn2/4p cooperate in the expression of Saccharomyces cerevisiae genes HSP26 and HSP104 in a gene- and stress type-dependent manner.

    PubMed

    Amorós, M; Estruch, F

    2001-03-01

    Saccharomyces cerevisiae possesses several transcription factors involved in the transcriptional activation of stress-induced genes. Among them, the heat shock factor (Hsf1p) and the zinc finger proteins of the general stress response (Msn2p and Msn4p) have been shown to play a major role in stress protection. Some heat shock protein (HSP) genes contain both heat shock elements (HSEs) and stress response elements (STREs), suggesting the involvement of both transcription factors in their regulation. Analysis of the stress-induced expression of two of these genes, HSP26 and HSP104, reveals that the contribution of Hsf1p and Msn2/4p is different depending on the gene and the stress condition.

  9. 40 CFR 25.8 - Responsiveness summaries.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Responsiveness summaries. 25.8 Section 25.8 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL PUBLIC PARTICIPATION IN PROGRAMS UNDER THE RESOURCE CONSERVATION AND RECOVERY ACT, THE SAFE DRINKING WATER ACT, AND THE CLEAN WATER...

  10. 40 CFR 258.12 - Wetlands.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Wetlands. 258.12 Section 258.12... Act or applicable State wetlands laws, the presumption that practicable alternative to the proposed... Species Act of 1973, and (iv) Violate any requirement under the Marine Protection, Research, and...

  11. 40 CFR 258.12 - Wetlands.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Wetlands. 258.12 Section 258.12... Act or applicable State wetlands laws, the presumption that practicable alternative to the proposed... Species Act of 1973, and (iv) Violate any requirement under the Marine Protection, Research, and...

  12. 40 CFR 258.12 - Wetlands.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Wetlands. 258.12 Section 258.12... Act or applicable State wetlands laws, the presumption that practicable alternative to the proposed... Species Act of 1973, and (iv) Violate any requirement under the Marine Protection, Research, and...

  13. 47 CFR 73.258 - Indicating instruments.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Indicating instruments. 73.258 Section 73.258 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES RADIO BROADCAST SERVICES FM....3549 may be filed with the FCC, Attention: Audio Division, Media Bureau, in Washington, DC for such...

  14. 40 CFR 258.21 - Cover material requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 258.21 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.21 Cover material requirements. (a... cover disposed solid waste with six inches of earthen material at the end of each operating day, or at...

  15. 40 CFR 258.55 - Assessment monitoring program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 258.55 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.55 Assessment... be shown that the removed constituents are not reasonably expected to be in or derived from the waste...

  16. 40 CFR 258.21 - Cover material requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 258.21 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.21 Cover material requirements. (a... cover disposed solid waste with six inches of earthen material at the end of each operating day, or at...

  17. TMEM258 Is a Component of the Oligosaccharyltransferase Complex Controlling ER Stress and Intestinal Inflammation.

    PubMed

    Graham, Daniel B; Lefkovith, Ariel; Deelen, Patrick; de Klein, Niek; Varma, Mukund; Boroughs, Angela; Desch, A Nicole; Ng, Aylwin C Y; Guzman, Gaelen; Schenone, Monica; Petersen, Christine P; Bhan, Atul K; Rivas, Manuel A; Daly, Mark J; Carr, Steven A; Wijmenga, Cisca; Xavier, Ramnik J

    2016-12-13

    Significant insights into disease pathogenesis have been gleaned from population-level genetic studies; however, many loci associated with complex genetic disease contain numerous genes, and phenotypic associations cannot be assigned unequivocally. In particular, a gene-dense locus on chromosome 11 (61.5-61.65 Mb) has been associated with inflammatory bowel disease, rheumatoid arthritis, and coronary artery disease. Here, we identify TMEM258 within this locus as a central regulator of intestinal inflammation. Strikingly, Tmem258 haploinsufficient mice exhibit severe intestinal inflammation in a model of colitis. At the mechanistic level, we demonstrate that TMEM258 is a required component of the oligosaccharyltransferase complex and is essential for N-linked protein glycosylation. Consequently, homozygous deficiency of Tmem258 in colonic organoids results in unresolved endoplasmic reticulum (ER) stress culminating in apoptosis. Collectively, our results demonstrate that TMEM258 is a central mediator of ER quality control and intestinal homeostasis. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

  18. 20 CFR 258.2 - Witnesses.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Witnesses. 258.2 Section 258.2 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD RETIREMENT ACT HEARINGS BEFORE THE BOARD... a delegation of authority to such examiner to require and compel the attendance of witnesses and the...

  19. 40 CFR 258.27 - Surface water requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Surface water requirements. 258.27... FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.27 Surface water requirements. MSWLF... wetlands, that violates any requirements of the Clean Water Act, including, but not limited to, the...

  20. 40 CFR 258.27 - Surface water requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Surface water requirements. 258.27... FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.27 Surface water requirements. MSWLF... wetlands, that violates any requirements of the Clean Water Act, including, but not limited to, the...

  1. 40 CFR 258.55 - Assessment monitoring program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Assessment monitoring program. 258.55 Section 258.55 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA... days; or (2) May demonstrate that a source other than a MSWLF unit caused the contamination, or that...

  2. 40 CFR 258.1 - Purpose, scope, and applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....1 Section 258.1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General § 258.1 Purpose, scope, and applicability. (a) The... Recovery Act (RCRA or the Act), as amended, for all municipal solid waste landfill (MSWLF) units and under...

  3. 40 CFR 258.23 - Explosive gases control.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 258.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.23 Explosive gases control. (a) Owners or... of MSWLFs that dispose of 20 tons of municipal solid waste per day or less, based on an annual...

  4. 40 CFR 258.1 - Purpose, scope, and applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....1 Section 258.1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General § 258.1 Purpose, scope, and applicability. (a) The... Recovery Act (RCRA or the Act), as amended, for all municipal solid waste landfill (MSWLF) units and under...

  5. 40 CFR 258.23 - Explosive gases control.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 258.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.23 Explosive gases control. (a) Owners or... of MSWLFs that dispose of 20 tons of municipal solid waste per day or less, based on an annual...

  6. 40 CFR 258.27 - Surface water requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Surface water requirements. 258.27... FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.27 Surface water requirements. MSWLF... wetlands, that violates any requirements of the Clean Water Act, including, but not limited to, the...

  7. Evolution of developmental roles of Pax2/5/8 paralogs after independent duplication in urochordate and vertebrate lineages.

    PubMed

    Bassham, Susan; Cañestro, Cristian; Postlethwait, John H

    2008-08-22

    Gene duplication provides opportunities for lineage diversification and evolution of developmental novelties. Duplicated genes generally either disappear by accumulation of mutations (nonfunctionalization), or are preserved either by the origin of positively selected functions in one or both duplicates (neofunctionalization), or by the partitioning of original gene subfunctions between the duplicates (subfunctionalization). The Pax2/5/8 family of important developmental regulators has undergone parallel expansion among chordate groups. After the divergence of urochordate and vertebrate lineages, two rounds of independent gene duplications resulted in the Pax2, Pax5, and Pax8 genes of most vertebrates (the sister group of the urochordates), and an additional duplication provided the pax2a and pax2b duplicates in teleost fish. Separate from the vertebrate genome expansions, a duplication also created two Pax2/5/8 genes in the common ancestor of ascidian and larvacean urochordates. To better understand mechanisms underlying the evolution of duplicated genes, we investigated, in the larvacean urochordate Oikopleura dioica, the embryonic gene expression patterns of Pax2/5/8 paralogs. We compared the larvacean and ascidian expression patterns to infer modular subfunctions present in the single pre-duplication Pax2/5/8 gene of stem urochordates, and we compared vertebrate and urochordate expression to infer the suite of Pax2/5/8 gene subfunctions in the common ancestor of olfactores (vertebrates + urochordates). Expression pattern differences of larvacean and ascidian Pax2/5/8 orthologs in the endostyle, pharynx and hindgut suggest that some ancestral gene functions have been partitioned differently to the duplicates in the two urochordate lineages. Novel expression in the larvacean heart may have resulted from the neofunctionalization of a Pax2/5/8 gene in the urochordates. Expression of larvacean Pax2/5/8 in the endostyle, in sites of epithelial remodeling, and in sensory tissues evokes like functions of Pax2, Pax5 and Pax8 in vertebrate embryos, and may indicate ancient origins for these functions in the chordate common ancestor. Comparative analysis of expression patterns of chordate Pax2/5/8 duplicates, rooted on the single-copy Pax2/5/8 gene of amphioxus, whose lineage diverged basally among chordates, provides new insights into the evolution and development of the heart, thyroid, pharynx, stomodeum and placodes in chordates; supports the controversial conclusion that the atrial siphon of ascidians and the otic placode in vertebrates are homologous; and backs the notion that Pax2/5/8 functioned in ancestral chordates to engineer epithelial fusions and perforations, including gill slit openings.

  8. Phages Infecting Vibrio vulnificus Are Abundant and Diverse in Oysters (Crassostrea virginica) Collected from the Gulf of Mexico

    PubMed Central

    DePaola, Angelo; Motes, Miles L.; Chan, Amy M.; Suttle, Curtis A.

    1998-01-01

    Phages infecting Vibrio vulnificus were abundant (>104 phages g of oyster tissue−1) throughout the year in oysters (Crassostrea virginica) collected from estuaries adjacent to the Gulf of Mexico (Apalachicola Bay, Fla.; Mobile Bay, Ala.; and Black Bay, La.). Estimates of abundance ranged from 101 to 105 phages g of oyster tissue−1 and were dependent on the bacterial strain used to assay the sample. V. vulnificus was near or below detection limits (<0.3 cell g−1) from January through March and was most abundant (103 to 104 cells g−1) during the summer and fall, when phage abundances also tended to be greatest. The phages isolated were specific to strains of V. vulnificus, except for one isolate that caused lysis in a few strains of V. parahaemolyticus. Based on morphological evidence obtained by transmission electron microscopy, the isolates belonged to the Podoviridae, Styloviridae, and Myoviridae, three families of double-stranded DNA phages. One newly described morphotype belonging to the Podoviridae appears to be ubiquitous in Gulf Coast oysters. Isolates of this morphotype have an elongated capsid (mean, 258 nm; standard deviation, 4 nm; n = 35), with some isolates having a relatively broad host range among strains of V. vulnificus. Results from this study indicate that a morphologically diverse group of phages which infect V. vulnificus is abundant and widely distributed in oysters from estuaries bordering the northeastern Gulf of Mexico. PMID:9435088

  9. Characterization of Escherichia coli and other Enterobacteriaceae in producer-distributor bulk milk.

    PubMed

    Ntuli, V; Njage, P M K; Buys, E M

    2016-12-01

    The current study was undertaken to characterize Escherichia coli and other Enterobacteriaceae in raw and pasteurized producer-distributor bulk milk (PDBM). A total of 258 samples were collected from purchase points in 8 provinces in South Africa. The samples were tested for antibiotic residues, phosphatase, total aerobic bacteria, coliforms, and E. coli counts. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for identification of isolates. Escherichia coli isolates were characterized for virulence factors, antimicrobial resistance, serotypes, and presumptive E. coli O157:H7. Antibiotic residues and alkaline phosphatase were detected in 2% of both raw and pasteurized PDBM (n=258) and 21% pasteurized PDBM (n=104) samples, respectively. A total of 729 isolates belonging to 21 genera and 59 species were identified. Escherichia coli, Enterobacter cloacae, Klebsiella oxytoca, and Raoultella ornithinolytica were the most abundant species. Spoilage Enterobacteriaceae species exceeded 50% of the total isolates. Escherichia coli was detected and isolated from 36% of the milk samples. Thirty-one E. coli isolates harbored virulence genes stx1/stx2 and 38% (n=121) were presumptive O157:H7. The prevalence of samples with presumptive shigatoxin producing E. coli was 10%. Antimicrobial-resistant E. coli isolates were detected in 70% of the milk samples with 36% of stx1/stx2 positive E. coli showing multi-drug resistance. Information obtained from the study will be used for modeling the public health risk posed by milkborne pathogens in PDBM, which in many cases is consumed by poor and vulnerable members of the population. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  10. 40 CFR 258.22 - Disease vector control.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Disease vector control. 258.22 Section... MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.22 Disease vector control. (a) Owners or operators of all MSWLF units must prevent or control on-site populations of disease vectors using techniques...

  11. 40 CFR 258.22 - Disease vector control.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Disease vector control. 258.22 Section... MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.22 Disease vector control. (a) Owners or operators of all MSWLF units must prevent or control on-site populations of disease vectors using techniques...

  12. 40 CFR 258.22 - Disease vector control.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Disease vector control. 258.22 Section... MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.22 Disease vector control. (a) Owners or operators of all MSWLF units must prevent or control on-site populations of disease vectors using techniques...

  13. 40 CFR 258.22 - Disease vector control.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Disease vector control. 258.22 Section... MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.22 Disease vector control. (a) Owners or operators of all MSWLF units must prevent or control on-site populations of disease vectors using techniques...

  14. 40 CFR 258.22 - Disease vector control.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Disease vector control. 258.22 Section... MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.22 Disease vector control. (a) Owners or operators of all MSWLF units must prevent or control on-site populations of disease vectors using techniques...

  15. 40 CFR 258.23 - Explosive gases control.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Explosive gases control. 258.23... FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.23 Explosive gases control. (a) Owners or... facility does not exceed 25 percent of the lower explosive limit for methane in facility structures...

  16. 40 CFR 258.23 - Explosive gases control.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Explosive gases control. 258.23... FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.23 Explosive gases control. (a) Owners or... facility does not exceed 25 percent of the lower explosive limit for methane in facility structures...

  17. 40 CFR 258.23 - Explosive gases control.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Explosive gases control. 258.23 Section... MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.23 Explosive gases control. (a) Owners or operators... does not exceed 25 percent of the lower explosive limit for methane in facility structures (excluding...

  18. 40 CFR 258.75 - Discounting.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... § 258.72(a), and/or corrective action costs in § 258.73(a) up to the rate of return for essentially risk free investments, net of inflation, under the following conditions: (a) The State Director determines... estimate of site life; and (d) Discounted cost estimates must be adjusted annually to reflect inflation and...

  19. 40 CFR 258.51 - Ground-water monitoring systems.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Ground-water monitoring systems. 258... CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.51 Ground-water monitoring systems. (a) A ground-water monitoring system must be installed that consists of a...

  20. 40 CFR 258.51 - Ground-water monitoring systems.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Ground-water monitoring systems. 258.51... FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.51 Ground-water monitoring systems. (a) A ground-water monitoring system must be installed that consists of a...

  1. 40 CFR 258.58 - Implementation of the corrective action program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258... implement a corrective action ground-water monitoring program that: (i) At a minimum, meet the requirements of an assessment monitoring program under § 258.55; (ii) Indicate the effectiveness of the corrective...

  2. 40 CFR 258.51 - Ground-water monitoring systems.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Ground-water monitoring systems. 258... CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.51 Ground-water monitoring systems. (a) A ground-water monitoring system must be installed that consists of a...

  3. 40 CFR 258.57 - Selection of remedy.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...-water quantity and quality; (iv) The potential damage to wildlife, crops, vegetation, and physical... MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.57 Selection of remedy... environment; (2) Attain the ground-water protection standard as specified pursuant to §§ 258.55 (h) or (i); (3...

  4. 40 CFR 258.57 - Selection of remedy.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...-water quantity and quality; (iv) The potential damage to wildlife, crops, vegetation, and physical... MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.57 Selection of remedy... environment; (2) Attain the ground-water protection standard as specified pursuant to §§ 258.55 (h) or (i); (3...

  5. 40 CFR 258.57 - Selection of remedy.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...-water quantity and quality; (iv) The potential damage to wildlife, crops, vegetation, and physical... MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.57 Selection of remedy... environment; (2) Attain the ground-water protection standard as specified pursuant to §§ 258.55 (h) or (i); (3...

  6. Relationship between Organizational Culture and Workplace Bullying among Korean Nurses.

    PubMed

    An, Yuseon; Kang, Jiyeon

    2016-09-01

    To identify the relationship between organizational culture and experience of workplace bullying among Korean nurses. Participants were 298 hospital nurses in Busan, South Korea. We assessed nursing organizational culture and workplace bullying among nurses using structured questionnaires from July 1 through August 15, 2014. Most participants considered their organizational culture as hierarchy-oriented (45.5%), followed by relation-oriented (36.0%), innovation-oriented (10.4%), and task-oriented (8.1%). According to the operational bullying criteria, the prevalence of workplace bullying was 15.8%. A multivariate logistic regression analysis revealed that the odds of being a victim of bullying were 2.58 times as high among nurses in a hierarchy-oriented culture as among nurses in a relation-oriented culture [95% confidence interval (1.12, 5.94)]. The results suggest that the types of nursing organizational culture are related to workplace bullying in Korean nurses. Further research is needed to develop interventions that can foster relation-oriented cultures to prevent workplace bullying in nurses. Copyright © 2016. Published by Elsevier B.V.

  7. Fatty acid composition of Juniperus species (Juniperus section) native to Turkey.

    PubMed

    Güvenç, Aysegül; Küçükboyaci, Nurgün; Gören, Ahmet Ceyhan

    2012-07-01

    Fatty acid compositions of seeds of five taxa of the Juniperus section of the genus Juniperus L. (Cupressaceae), i. e. J. drupacea Lab., J. communis L. var. communis, J. communis var. saxatilis Pall., J. oxycedrus L. subsp. oxycedrus, and J. oxycedrus subsp. macrocarpa (Sibth. & Sm.) Ball, were investigated. Methyl ester derivatized fatty acids of the lipophylic extracts of the five species were comparatively analyzed by capillary gas chromatography-mass spectrometry (GC-MS). Juniperus taxa showed uniform fatty acid patterns, among which linoleic (25.8 - 32.5%), pinolenic (11.9 - 24.1%) and oleic acids (12.4 - 17.2%) were determined to be the main fractions in the seed oils. Juniperonic acid was found to be remarkably high in J. communis var. saxatilis (11.4%), J. oxycedrus subsp. oxycedrus (10.4%), and J. communis var. communis (10.1%). To the best of our knowledge, the present work discloses the first report on the fatty acid compositions of seeds of this Juniperus section grown in Turkey.

  8. Dramatically enhanced non-Ohmic properties and maximum stored energy density in ceramic-metal nanocomposites: CaCu3Ti4O12/Au nanoparticles

    PubMed Central

    2013-01-01

    Non-Ohmic and dielectric properties of a novel CaCu3Ti4O12/Au nanocomposite were investigated. Introduction of 2.5 vol.% Au nanoparticles in CaCu3Ti4O12 ceramics significantly reduced the loss tangent while its dielectric permittivity remained unchanged. The non-Ohmic properties of CaCu3Ti4O12/Au (2.5 vol.%) were dramatically improved. A nonlinear coefficient of ≈ 17.7 and breakdown electric field strength of 1.25 × 104 V/m were observed. The maximum stored energy density was found to be 25.8 kJ/m3, which is higher than that of pure CaCu3Ti4O12 by a factor of 8. Au addition at higher concentrations resulted in degradation of dielectric and non-Ohmic properties, which is described well by percolation theory. PMID:24257060

  9. Epidemic potential of Escherichia coli ST131 and Klebsiella pneumoniae ST258: a systematic review and meta-analysis

    PubMed Central

    Dautzenberg, M J D; Haverkate, M R; Bonten, M J M; Bootsma, M C J

    2016-01-01

    Objectives Observational studies have suggested that Escherichia coli sequence type (ST) 131 and Klebsiella pneumoniae ST258 have hyperendemic properties. This would be obvious from continuously high incidence and/or prevalence of carriage or infection with these bacteria in specific patient populations. Hyperendemicity could result from increased transmissibility, longer duration of infectiousness, and/or higher pathogenic potential as compared with other lineages of the same species. The aim of our research is to quantitatively estimate these critical parameters for E. coli ST131 and K. pneumoniae ST258, in order to investigate whether E. coli ST131 and K. pneumoniae ST258 are truly hyperendemic clones. Primary outcome measures A systematic literature search was performed to assess the evidence of transmissibility, duration of infectiousness, and pathogenicity for E. coli ST131 and K. pneumoniae ST258. Meta-regression was performed to quantify these characteristics. Results The systematic literature search yielded 639 articles, of which 19 data sources provided information on transmissibility (E. coli ST131 n=9; K. pneumoniae ST258 n=10)), 2 on duration of infectiousness (E. coli ST131 n=2), and 324 on pathogenicity (E. coli ST131 n=285; K. pneumoniae ST258 n=39). Available data on duration of carriage and on transmissibility were insufficient for quantitative assessment. In multivariable meta-regression E. coli isolates causing infection were associated with ST131, compared to isolates only causing colonisation, suggesting that E. coli ST131 can be considered more pathogenic than non-ST131 isolates. Date of isolation, location and resistance mechanism also influenced the prevalence of ST131. E. coli ST131 was 3.2 (95% CI 2.0 to 5.0) times more pathogenic than non-ST131. For K. pneumoniae ST258 there were not enough data for meta-regression assessing the influence of colonisation versus infection on ST258 prevalence. Conclusions With the currently available data, it cannot be confirmed nor rejected, that E. coli ST131 or K. pneumoniae ST258 are hyperendemic clones. PMID:26988349

  10. 42 CFR 441.258 - Consent form requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Consent form requirements. 441.258 Section 441.258... informed consent as set forth on the consent form; and (iii) To the best of his or her knowledge and belief...) To the best of his or her knowledge and belief, the individual appeared mentally competent and...

  11. 27 CFR 44.258 - To officers of the armed forces for subsequent exportation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... forces for subsequent exportation. 44.258 Section 44.258 Alcohol, Tobacco Products and Firearms ALCOHOL... forces for subsequent exportation. Where cigars are withdrawn from a customs warehouse for delivery to officers of the armed forces of the United States in this country for subsequent shipment to, and use by...

  12. 27 CFR 44.258 - To officers of the armed forces for subsequent exportation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... forces for subsequent exportation. 44.258 Section 44.258 Alcohol, Tobacco Products and Firearms ALCOHOL... forces for subsequent exportation. Where cigars are withdrawn from a customs warehouse for delivery to officers of the armed forces of the United States in this country for subsequent shipment to, and use by...

  13. 40 CFR 258.72 - Financial assurance for post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... care. 258.72 Section 258.72 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID... assurance for post-closure care. (a) The owner or operator must have a detailed written estimate, in current dollars, of the cost of hiring a third party to conduct post-closure care for the MSWLF unit in compliance...

  14. 37 CFR 258.4 - Royalty fee for secondary transmission of digital signals of broadcast stations by satellite...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... transmission of digital signals of broadcast stations by satellite carriers. 258.4 Section 258.4 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES... Royalty fee for secondary transmission of digital signals of broadcast stations by satellite carriers. (a...

  15. 37 CFR 258.4 - Royalty fee for secondary transmission of digital signals of broadcast stations by satellite...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... transmission of digital signals of broadcast stations by satellite carriers. 258.4 Section 258.4 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES... Royalty fee for secondary transmission of digital signals of broadcast stations by satellite carriers. (a...

  16. 37 CFR 258.4 - Royalty fee for secondary transmission of digital signals of broadcast stations by satellite...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... transmission of digital signals of broadcast stations by satellite carriers. 258.4 Section 258.4 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES... Royalty fee for secondary transmission of digital signals of broadcast stations by satellite carriers. (a...

  17. 37 CFR 258.4 - Royalty fee for secondary transmission of digital signals of broadcast stations by satellite...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... transmission of digital signals of broadcast stations by satellite carriers. 258.4 Section 258.4 Patents, Trademarks, and Copyrights U.S. COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL... Royalty fee for secondary transmission of digital signals of broadcast stations by satellite carriers. (a...

  18. 37 CFR 258.4 - Royalty fee for secondary transmission of digital signals of broadcast stations by satellite...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... transmission of digital signals of broadcast stations by satellite carriers. 258.4 Section 258.4 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES... Royalty fee for secondary transmission of digital signals of broadcast stations by satellite carriers. (a...

  19. Evolution of developmental roles of Pax2/5/8 paralogs after independent duplication in urochordate and vertebrate lineages

    PubMed Central

    Bassham, Susan; Cañestro, Cristian; Postlethwait, John H

    2008-01-01

    Background Gene duplication provides opportunities for lineage diversification and evolution of developmental novelties. Duplicated genes generally either disappear by accumulation of mutations (nonfunctionalization), or are preserved either by the origin of positively selected functions in one or both duplicates (neofunctionalization), or by the partitioning of original gene subfunctions between the duplicates (subfunctionalization). The Pax2/5/8 family of important developmental regulators has undergone parallel expansion among chordate groups. After the divergence of urochordate and vertebrate lineages, two rounds of independent gene duplications resulted in the Pax2, Pax5, and Pax8 genes of most vertebrates (the sister group of the urochordates), and an additional duplication provided the pax2a and pax2b duplicates in teleost fish. Separate from the vertebrate genome expansions, a duplication also created two Pax2/5/8 genes in the common ancestor of ascidian and larvacean urochordates. Results To better understand mechanisms underlying the evolution of duplicated genes, we investigated, in the larvacean urochordate Oikopleura dioica, the embryonic gene expression patterns of Pax2/5/8 paralogs. We compared the larvacean and ascidian expression patterns to infer modular subfunctions present in the single pre-duplication Pax2/5/8 gene of stem urochordates, and we compared vertebrate and urochordate expression to infer the suite of Pax2/5/8 gene subfunctions in the common ancestor of olfactores (vertebrates + urochordates). Expression pattern differences of larvacean and ascidian Pax2/5/8 orthologs in the endostyle, pharynx and hindgut suggest that some ancestral gene functions have been partitioned differently to the duplicates in the two urochordate lineages. Novel expression in the larvacean heart may have resulted from the neofunctionalization of a Pax2/5/8 gene in the urochordates. Expression of larvacean Pax2/5/8 in the endostyle, in sites of epithelial remodeling, and in sensory tissues evokes like functions of Pax2, Pax5 and Pax8 in vertebrate embryos, and may indicate ancient origins for these functions in the chordate common ancestor. Conclusion Comparative analysis of expression patterns of chordate Pax2/5/8 duplicates, rooted on the single-copy Pax2/5/8 gene of amphioxus, whose lineage diverged basally among chordates, provides new insights into the evolution and development of the heart, thyroid, pharynx, stomodeum and placodes in chordates; supports the controversial conclusion that the atrial siphon of ascidians and the otic placode in vertebrates are homologous; and backs the notion that Pax2/5/8 functioned in ancestral chordates to engineer epithelial fusions and perforations, including gill slit openings. PMID:18721460

  20. 40 CFR 258.62 - Approval of site-specific flexibility requests in Indian country.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Closure and Post-Closure Care § 258.62... waste landfill owned and operated by Lake County on the Confederated Salish and Kootenai Tribes... equivalent reduction in infiltration as the infiltration layer specified in § 258.60(a)(1) and (a)(2), and...

  1. 30 CFR 250.258 - What information on the onshore support facilities you will use must accompany the DPP or DOCD?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 2 2011-07-01 2011-07-01 false What information on the onshore support facilities you will use must accompany the DPP or DOCD? 250.258 Section 250.258 Mineral Resources BUREAU OF... AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Plans and Information Contents of Development...

  2. 30 CFR 250.258 - What information on the onshore support facilities you will use must accompany the DPP or DOCD?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What information on the onshore support facilities you will use must accompany the DPP or DOCD? 250.258 Section 250.258 Mineral Resources MINERALS... CONTINENTAL SHELF Plans and Information Contents of Development and Production Plans (dpp) and Development...

  3. Chemical experiments with superheavy elements.

    PubMed

    Türler, Andreas

    2010-01-01

    Unnoticed by many chemists, the Periodic Table of the Elements has been extended significantly in the last couple of years and the 7th period has very recently been completed with eka-Rn (element 118) currently being the heaviest element whose synthesis has been reported. These 'superheavy' elements (also called transactinides with atomic number > or = 104 (Rf)) have been artificially synthesized in fusion reactions at accelerators in minute quantities of a few single atoms. In addition, all isotopes of the transactinide elements are radioactive and decay with rather short half-lives. Nevertheless, it has been possible in some cases to investigate experimentally chemical properties of transactinide elements and even synthesize simple compounds. The experimental investigation of superheavy elements is especially intriguing, since theoretical calculations predict significant deviations from periodic trends due to the influence of strong relativistic effects. In this contribution first experiments with hassium (Hs, atomic number 108), copernicium (Cn, atomic number 112) and element 114 (eka-Pb) are reviewed.

  4. A convenient method for the quantitative determination of elemental sulfur in coal by HPLC analysis of perchloroethylene extracts

    USGS Publications Warehouse

    Buchanan, D.H.; Coombs, K.J.; Murphy, P.M.; Chaven, C.

    1993-01-01

    A convenient method for the quantitative determination of elemental sulfur in coal is described. Elemental sulfur is extracted from the coal with hot perchloroethylene (PCE) (tetrachloroethene, C2Cl4) and quantitatively determined by HPLC analysis on a C18 reverse-phase column using UV detection. Calibration solutions were prepared from sublimed sulfur. Results of quantitative HPLC analyses agreed with those of a chemical/spectroscopic analysis. The HPLC method was found to be linear over the concentration range of 6 ?? 10-4 to 2 ?? 10-2 g/L. The lower detection limit was 4 ?? 10-4 g/L, which for a coal sample of 20 g is equivalent to 0.0006% by weight of coal. Since elemental sulfur is known to react slowly with hydrocarbons at the temperature of boiling PCE, standard solutions of sulfur in PCE were heated with coals from the Argonne Premium Coal Sample program. Pseudo-first-order uptake of sulfur by the coals was observed over several weeks of heating. For the Illinois No. 6 premium coal, the rate constant for sulfur uptake was 9.7 ?? 10-7 s-1, too small for retrograde reactions between solubilized sulfur and coal to cause a significant loss in elemental sulfur isolated during the analytical extraction. No elemental sulfur was produced when the following pure compounds were heated to reflux in PCE for up to 1 week: benzyl sulfide, octyl sulfide, thiane, thiophene, benzothiophene, dibenzothiophene, sulfuric acid, or ferrous sulfate. A sluury of mineral pyrite in PCE contained elemental sulfur which increased in concentration with heating time. ?? 1993 American Chemical Society.

  5. Empirical analysis of knowledge bases to support structured output in the Arden syntax.

    PubMed

    Jenders, Robert A

    2013-01-01

    Structured output has been suggested for the Arden Syntax to facilitate interoperability. Tabulate the components of WRITE statements in a corpus of medical logic modules (MLMs)in order to validate requiring structured output. WRITE statements were tabulated in 258 MLMs from 2 organizations. In a total of 351 WRITE statements, email destinations (226) predominated, and 39 orders and 40 coded output elements also were tabulated. Free-text strings predominated as the message data. Arden WRITE statements contain considerable potentially structured data now included as free text. A future, normative structured WRITE statement must address a variety of data types and destinations.

  6. Diversity, virulence, and antimicrobial resistance of the KPC-producing Klebsiella pneumoniae ST307 clone.

    PubMed

    Villa, Laura; Feudi, Claudia; Fortini, Daniela; Brisse, Sylvain; Passet, Virginie; Bonura, Celestino; Endimiani, Andrea; Mammina, Caterina; Ocampo, Ana Maria; Jimenez, Judy Natalia; Doumith, Michel; Woodford, Neil; Hopkins, Katie; Carattoli, Alessandra

    2017-04-01

    The global spread of Klebsiella pneumoniae producing Klebsiella pneumoniae carbapenemase (KPC) has been mainly associated with the dissemination of high-risk clones. In the last decade, hospital outbreaks involving KPC-producing K. pneumoniae have been predominantly attributed to isolates belonging to clonal group (CG) 258. However, results of recent epidemiological analysis indicate that KPC-producing sequence type (ST) 307, is emerging in different parts of the world and is a candidate to become a prevalent high-risk clone in the near future. Here we show that the ST307 genome encodes genetic features that may provide an advantage in adaptation to the hospital environment and the human host. Sequence analysis revealed novel plasmid-located virulence factors, including a cluster for glycogen synthesis. Glycogen production is considered to be one of the possible adaptive responses to long-term survival and growth in environments outside the host. Chromosomally-encoded virulence traits in the clone comprised fimbriae, an integrative conjugative element carrying the yersiniabactin siderophore, and two different capsular loci. Compared with the ST258 clone, capsulated ST307 isolates showed higher resistance to complement-mediated killing. The acquired genetic features identified in the genome of this new emerging clone may contribute to increased persistence of ST307 in the hospital environment and shed light on its potential epidemiological success.

  7. Application of human reliability analysis to nursing errors in hospitals.

    PubMed

    Inoue, Kayoko; Koizumi, Akio

    2004-12-01

    Adverse events in hospitals, such as in surgery, anesthesia, radiology, intensive care, internal medicine, and pharmacy, are of worldwide concern and it is important, therefore, to learn from such incidents. There are currently no appropriate tools based on state-of-the art models available for the analysis of large bodies of medical incident reports. In this study, a new model was developed to facilitate medical error analysis in combination with quantitative risk assessment. This model enables detection of the organizational factors that underlie medical errors, and the expedition of decision making in terms of necessary action. Furthermore, it determines medical tasks as module practices and uses a unique coding system to describe incidents. This coding system has seven vectors for error classification: patient category, working shift, module practice, linkage chain (error type, direct threat, and indirect threat), medication, severity, and potential hazard. Such mathematical formulation permitted us to derive two parameters: error rates for module practices and weights for the aforementioned seven elements. The error rate of each module practice was calculated by dividing the annual number of incident reports of each module practice by the annual number of the corresponding module practice. The weight of a given element was calculated by the summation of incident report error rates for an element of interest. This model was applied specifically to nursing practices in six hospitals over a year; 5,339 incident reports with a total of 63,294,144 module practices conducted were analyzed. Quality assurance (QA) of our model was introduced by checking the records of quantities of practices and reproducibility of analysis of medical incident reports. For both items, QA guaranteed legitimacy of our model. Error rates for all module practices were approximately of the order 10(-4) in all hospitals. Three major organizational factors were found to underlie medical errors: "violation of rules" with a weight of 826 x 10(-4), "failure of labor management" with a weight of 661 x 10(-4), and "defects in the standardization of nursing practices" with a weight of 495 x 10(-4).

  8. Mutagenicity evaluation of forty-one metal salts by the umu test.

    PubMed

    Yamamoto, Akiko; Kohyama, Yuko; Hanawa, Takao

    2002-01-01

    Metallic biomaterials implanted in a human body may corrode and wear, releasing metal ions and debris which may induce adverse reactions such as inflammation, allergy, neoplastic formation, developmental malformation, etc. Mutagenicity is a very fundamental and important toxicity related to carcinogenicity and reproductive/developmental toxicity because the damages to genes or DNA can be a cause of carcinogenesis and developmental abnormalities. However, available mutagenic data on metallic ions and compounds are restricted to the number of elements. Therefore, to obtain the systematic data necessary for metal ion mutagenicity, 41 metal salts encompassing 36 metals and 5 metallic elements tested with different valences, were evaluated on their mutagenicity by a microbial test, the umu test. As a result, K(2)Cr(2)O(7), RhCl(3), IrCl(4), and MgCl(2) are positive without metabolic activation. Concentrations having the maximum mutagenic effect (C(max)) are 9.65 x 10(-5), 1.00 x 10(-4), 3.11 x 10(-3), 4.12 x 10(-3) mol. L(-1), respectively. CuCl(2), VCl(3), CuCl, RhCl(3), K(2)Cr(2)O(7), and IrCl(4) are positive with metabolic activation by S-9 mix with C(max) of 1.60 x 10(-5), 3.91 x 10(-5), 1.57 x 10(-4), 2.00 x 10(-4), 3.86 x 10(-4), 1.56 x 10(-2) mol. L(-1), respectively. Thirty-five metal salts were negative for tests performed both with and without metabolic activation, whereas it was impossible to evaluate the mutagenicity of MoCl(5) and ZrCl(4) by the umu test because of their colorimetric reaction to testing reagents. Copyright 2001 John Wiley & Sons, Inc.

  9. Echolocation-Based Foraging by Harbor Porpoises and Sperm Whales, Including Effects on Noise and Acoustic Propagation

    DTIC Science & Technology

    2008-09-01

    Behavioural Point Process Data 234 Appendix B: Matlab Code 258 Matlab Code Used in Chapter 2 (Porpoise Prey Capture Analysis) 258 Click Extraction and...Measurement of Click Properties 258 Envelope-based Click Detector 262 Matlab Code Used in Chapter 3 (Transmission Loss in Porpoise Habitats) ..267...Click Extraction from Data Wavefiles 267 Click Level Determination (Grand Manan Datasets) 270 Click Level Determination (Danish Datasets) 287 Matlab

  10. 7 CFR 1717.860 - Lien accommodations and subordinations under section 306E of the RE Act.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... accommodation and including the amount and maturity of the proposed loan, a general description of the... model codes for any buildings to be constructed, as required by 7 CFR 1792.104. All other elements of an...

  11. The effect of pasteurization on trace elements in donor breast milk.

    PubMed

    Mohd-Taufek, N; Cartwright, D; Davies, M; Hewavitharana, A K; Koorts, P; McConachy, H; Shaw, P N; Sumner, R; Whitfield, K

    2016-10-01

    Premature infants often receive pasteurized donor human milk when mothers are unable to provide their own milk. This study aims to establish the effect of the pasteurization process on a range of trace elements in donor milk. Breast milk was collected from 16 mothers donating to the milk bank at the Royal Brisbane and Women's Hospital. Samples were divided into pre- and post-pasteurization aliquots and were Holder pasteurized. Inductively coupled plasma mass spectrometry was used to analyze the trace elements zinc (Zn), copper (Cu), selenium (Se), manganese (Mn), iodine (I), iron (Fe), molybdenum (Mo) and bromine (Br). Differences in trace elements pre- and post-pasteurization were analyzed. No significant differences were found between the trace elements tested pre- and post-pasteurization, except for Fe (P<0.05). The median (interquartile range, 25 to 75%; μg l(-1)) of trace elements for pre- and post- pasteurization aliquots were-Zn: 1639 (888-4508), 1743 (878-4143), Cu: 360 (258-571), 367 (253-531), Se: 12.34 (11.73-17.60), 12.62 (11.94-16.64), Mn: (1.48 (1.01-1.75), 1.49 (1.11-1.75), I (153 (94-189), 158 (93-183), Fe (211 (171-277), 194 (153-253), Mo (1.46 (0.37-2.99), 1.42 (0.29-3.73) and Br (1066 (834-1443), 989 (902-1396). Pasteurization had minimal effect on several trace elements in donor breast milk but high levels of inter-donor variability of trace elements were observed. The observed decrease in the iron content of pasteurized donor milk is, however, unlikely to be clinically relevant.

  12. 7 CFR 868.258 - Moisture.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... FOR CERTAIN AGRICULTURAL COMMODITIES United States Standards for Brown Rice for Processing Principles Governing Application of Standards § 868.258 Moisture. Water content in brown rice for processing as...

  13. 36 CFR 6.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Waste Landfills at 40 CFR 258.60 and 258.61. Compostible materials means organic substances that decay... water, air, soils, geological features, including subsurface strata, the natural processes and...

  14. 36 CFR 6.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Waste Landfills at 40 CFR 258.60 and 258.61. Compostible materials means organic substances that decay... water, air, soils, geological features, including subsurface strata, the natural processes and...

  15. 36 CFR 6.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Waste Landfills at 40 CFR 258.60 and 258.61. Compostible materials means organic substances that decay... water, air, soils, geological features, including subsurface strata, the natural processes and...

  16. Regulation of the Incorporation of Tissue Factor into Microparticles by Serine Phosphorylation of the Cytoplasmic Domain of Tissue Factor*

    PubMed Central

    Collier, Mary E. W.; Ettelaie, Camille

    2011-01-01

    The mechanisms that regulate the incorporation and release of tissue factors (TFs) into cell-derived microparticles are as yet unidentified. In this study, we have explored the regulation of TF release into microparticles by the phosphorylation of serine residues within the cytoplasmic domain of TF. Wild-type and mutant forms of TF, containing alanine and aspartate substitutions at Ser253 and Ser258, were overexpressed in coronary artery and dermal microvascular endothelial cells and microparticle release stimulated with PAR2 agonist peptide (PAR2-AP). The release of TF antigen and activity was then monitored. In addition, the phosphorylation state of the two serine residues within the released microparticles and the cells was monitored for 150 min. The release of wild-type TF as procoagulant microparticles peaked at 90 min and declined thereafter in both cell types. The TF within these microparticles was phosphorylated at Ser253 but not at Ser258. Aspartate substitution of Ser253 resulted in rapid release of TF antigen but not activity, whereas TF release was reduced and delayed by alanine substitution of Ser253 or aspartate substitution of Ser258. Alanine substitution of Ser258 prolonged the release of TF following PAR2-AP activation. The release of TF was concurrent with phosphorylation of Ser253 and was followed by dephosphorylation at 120 min and phosphorylation of Ser258. We propose a sequential mechanism in which the phosphorylation of Ser253 through PAR2 activation results in the incorporation of TF into microparticles, simultaneously inducing Ser258 phosphorylation. Phosphorylation of Ser258 in turn promotes the dephosphorylation of Ser253 and suppresses the release of TF. PMID:21310953

  17. Vascular disease-causing mutation R258C in ACTA2 disrupts actin dynamics and interaction with myosin

    PubMed Central

    Lu, Hailong; Fagnant, Patricia M.; Bookwalter, Carol S.; Joel, Peteranne; Trybus, Kathleen M.

    2015-01-01

    Point mutations in vascular smooth muscle α-actin (SM α-actin), encoded by the gene ACTA2, are the most prevalent cause of familial thoracic aortic aneurysms and dissections (TAAD). Here, we provide the first molecular characterization, to our knowledge, of the effect of the R258C mutation in SM α-actin, expressed with the baculovirus system. Smooth muscles are unique in that force generation requires both interaction of stable actin filaments with myosin and polymerization of actin in the subcortical region. Both aspects of R258C function therefore need investigation. Total internal reflection fluorescence (TIRF) microscopy was used to quantify the growth of single actin filaments as a function of time. R258C filaments are less stable than WT and more susceptible to severing by cofilin. Smooth muscle tropomyosin offers little protection from cofilin cleavage, unlike its effect on WT actin. Unexpectedly, profilin binds tighter to the R258C monomer, which will increase the pool of globular actin (G-actin). In an in vitro motility assay, smooth muscle myosin moves R258C filaments more slowly than WT, and the slowing is exacerbated by smooth muscle tropomyosin. Under loaded conditions, small ensembles of myosin are unable to produce force on R258C actin-tropomyosin filaments, suggesting that tropomyosin occupies an inhibitory position on actin. Many of the observed defects cannot be explained by a direct interaction with the mutated residue, and thus the mutation allosterically affects multiple regions of the monomer. Our results align with the hypothesis that defective contractile function contributes to the pathogenesis of TAAD. PMID:26153420

  18. High-speed photography of plasma during excimer laser-tissue interaction.

    PubMed

    Murray, Andrea K; Dickinson, Mark R

    2004-08-07

    During high fluence laser-tissue interaction, ablation of tissue occurs, debris is removed from the ablation site and is then ejected at high velocity. This debris may be observed as a combination of luminous plasma and non-luminous plume, both of which have the potential to shield the ablation site. This study examined the role of ablation debris in shielding the tissue and determined its effects on the ablation rate over a range of laser pulse energies, pulse repetition rates and pulse numbers for dentine; the velocity differences between hard and soft tissues were also examined. High-speed photography was carried out at up to 1 x 10(8) frames per second. A maximum velocity of 2.58 +/- 0.52 x 10(4) m s(-1) was recorded for dentine debris within the first 10 ns following ejection. The maximum duration of tissue shielding due to a single pulse, determined by attenuation of a probe beam, was found to be approximately 7 ms, approximately 80 micros of which was due to luminous plasma and the remainder due to the non-luminous plume.

  19. 7 CFR 868.258 - Moisture.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Governing Application of Standards § 868.258 Moisture. Water content in brown rice for processing as... purpose of this paragraph, “approved device” shall include the Motomco Moisture Meter and any other...

  20. 40 CFR 258.2 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...; stone, glass, clay, and concrete products; textile manufacturing; transportation equipment; and water... the MSWLF unit meets the conditions of § 258.1(f)(1). Open burning means the combustion of solid waste...

  1. 40 CFR 258.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...; stone, glass, clay, and concrete products; textile manufacturing; transportation equipment; and water... the MSWLF unit meets the conditions of § 258.1(f)(1). Open burning means the combustion of solid waste...

  2. 40 CFR 258.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...; stone, glass, clay, and concrete products; textile manufacturing; transportation equipment; and water... the MSWLF unit meets the conditions of § 258.1(f)(1). Open burning means the combustion of solid waste...

  3. Comparative Genomics of Recent Shiga Toxin-Producing Escherichia coli O104:H4: Short-Term Evolution of an Emerging Pathogen

    PubMed Central

    Grad, Yonatan H.; Godfrey, Paul; Cerquiera, Gustavo C.; Mariani-Kurkdjian, Patricia; Gouali, Malika; Bingen, Edouard; Shea, Terrence P.; Haas, Brian J.; Griggs, Allison; Young, Sarah; Zeng, Qiandong; Lipsitch, Marc; Waldor, Matthew K.; Weill, François-Xavier; Wortman, Jennifer R.; Hanage, William P.

    2013-01-01

    ABSTRACT The large outbreak of diarrhea and hemolytic uremic syndrome (HUS) caused by Shiga toxin-producing Escherichia coli O104:H4 in Europe from May to July 2011 highlighted the potential of a rarely identified E. coli serogroup to cause severe disease. Prior to the outbreak, there were very few reports of disease caused by this pathogen and thus little known of its diversity and evolution. The identification of cases of HUS caused by E. coli O104:H4 in France and Turkey after the outbreak and with no clear epidemiological links raises questions about whether these sporadic cases are derived from the outbreak. Here, we report genome sequences of five independent isolates from these cases and results of a comparative analysis with historical and 2011 outbreak isolates. These analyses revealed that the five isolates are not derived from the outbreak strain; however, they are more closely related to the outbreak strain and each other than to isolates identified prior to the 2011 outbreak. Over the short time scale represented by these closely related organisms, the majority of genome variation is found within their mobile genetic elements: none of the nine O104:H4 isolates compared here contain the same set of plasmids, and their prophages and genomic islands also differ. Moreover, the presence of closely related HUS-associated E. coli O104:H4 isolates supports the contention that fully virulent O104:H4 isolates are widespread and emphasizes the possibility of future food-borne E. coli O104:H4 outbreaks. PMID:23341549

  4. E258K HCM-causing mutation in cardiac MyBP-C reduces contractile force and accelerates twitch kinetics by disrupting the cMyBP-C and myosin S2 interaction.

    PubMed

    De Lange, Willem J; Grimes, Adrian C; Hegge, Laura F; Spring, Alexander M; Brost, Taylor M; Ralphe, J Carter

    2013-09-01

    Mutations in cardiac myosin binding protein C (cMyBP-C) are prevalent causes of hypertrophic cardiomyopathy (HCM). Although HCM-causing truncation mutations in cMyBP-C are well studied, the growing number of disease-related cMyBP-C missense mutations remain poorly understood. Our objective was to define the primary contractile effect and molecular disease mechanisms of the prevalent cMyBP-C E258K HCM-causing mutation in nonremodeled murine engineered cardiac tissue (mECT). Wild-type and human E258K cMyBP-C were expressed in mECT lacking endogenous mouse cMyBP-C through adenoviral-mediated gene transfer. Expression of E258K cMyBP-C did not affect cardiac cell survival and was appropriately incorporated into the cardiac sarcomere. Functionally, expression of E258K cMyBP-C caused accelerated contractile kinetics and severely compromised twitch force amplitude in mECT. Yeast two-hybrid analysis revealed that E258K cMyBP-C abolished interaction between the N terminal of cMyBP-C and myosin heavy chain sub-fragment 2 (S2). Furthermore, this mutation increased the affinity between the N terminal of cMyBP-C and actin. Assessment of phosphorylation of three serine residues in cMyBP-C showed that aberrant phosphorylation of cMyBP-C is unlikely to be responsible for altering these interactions. We show that the E258K mutation in cMyBP-C abolishes interaction between N-terminal cMyBP-C and myosin S2 by directly disrupting the cMyBP-C-S2 interface, independent of cMyBP-C phosphorylation. Similar to cMyBP-C ablation or phosphorylation, abolition of this inhibitory interaction accelerates contractile kinetics. Additionally, the E258K mutation impaired force production of mECT, which suggests that in addition to the loss of physiological function, this mutation disrupts contractility possibly by tethering the thick and thin filament or acting as an internal load.

  5. Molecular characterization, genomic distribution and evolutionary dynamics of Short INterspersed Elements in the termite genome.

    PubMed

    Luchetti, Andrea; Mantovani, Barbara

    2011-02-01

    Short INterspersed Elements (SINEs) in invertebrates, and especially in animal inbred genomes such that of termites, are poorly known; in this paper we characterize three new SINE families (Talub, Taluc and Talud) through the analyses of 341 sequences, either isolated from the Reticulitermes lucifugus genome or drawn from EST Genbank collection. We further add new data to the only isopteran element known so far, Talua. These SINEs are tRNA-derived elements, with an average length ranging from 258 to 372 bp. The tails are made up by poly(A) or microsatellite motifs. Their copy number varies from 7.9 × 10(3) to 10(5) copies, well within the range observed for other metazoan genomes. Species distribution, age and target site duplication analysis indicate Talud as the oldest, possibly inactive SINE originated before the onset of Isoptera (~150 Myr ago). Taluc underwent to substantial sequence changes throughout the evolution of termites and data suggest it was silenced and then re-activated in the R. lucifugus lineage. Moreover, Taluc shares a conserved sequence block with other unrelated SINEs, as observed for some vertebrate and cephalopod elements. The study of genomic environment showed that insertions are mainly surrounded by microsatellites and other SINEs, indicating a biased accumulation within non-coding regions. The evolutionary dynamics of Talu~ elements is explained through selective mechanisms acting in an inbred genome; in this respect, the study of termites' SINEs activity may provide an interesting framework to address the (co)evolution of mobile elements and the host genome.

  6. RCRA, superfund and EPCRA hotline training module. Introduction to: Municipal solid waste disposal facility criteria updated July 1996

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1996-07-01

    The module provides a summary of the regulatory criteria for municipal solid waste landfills (MSWLFs) and provides the statutory authority under RCRA and the Clean Water Act (CWA) directing EPA to develop the MSWLF criteria in 40 CFR Part 258. It gives the part 258 effective date and the compliance dates for providing demonstrations to satisfy individual regulatory requirements. It identifies the types of facilities that qualify for the small landfill exemption. It explains the requirements of each subpart of part 258 as they apply to states with EPA-approved MSWLF permit programs and states without approved permit programs. It comparesmore » the MSWLF environmental performance standards described in part 258 to the corresponding requirements for hazardous waste TSDFs in part 264, which are generally more stringent.« less

  7. Lumped element kinetic inductance detectors based on two-gap MgB2 thin films

    NASA Astrophysics Data System (ADS)

    Yang, C.; Niu, R. R.; Guo, Z. S.; Cai, X. W.; Chu, H. M.; Yang, K.; Wang, Y.; Feng, Q. R.; Gan, Z. Z.

    2018-01-01

    Lumped element kinetic inductance detectors (LEKIDs) are made from a single layer superconducting thin film. Because of their low noise and highly multiplexibility, LEKIDs provide a sensitive technology for the detection of millimeter and submillimeter waves. In this work, a 5-pixel 50-nm-thick MgB2 array is made. The microwave properties of the array are measured under dark conditions. We show that the loaded quality factor Q of the resonant circuit is 30 000 at 7.5 K, which is comparable to that of lower-operating-temperature (usually several hundred mK) LEKIDs made from superconductors such as Al and Nb. Moreover, the temperature dependence of resonance frequency gives the two-gap character of MgB2, Δπ (0) = 2.58 meV and Δσ (0) = 8.26 meV. The gap frequency (f = 2Δ/h) indicates that MgB2 LEKIDs have a promising application on terahertz detection.

  8. Finite element analysis to compare complete denture and implant-retained overdentures with different attachment systems.

    PubMed

    Barão, Valentim Adelino Ricardo; Assunção, Wirley Gonçalves; Tabata, Lucas Fernando; Delben, Juliana Aparecida; Gomes, Erica Alves; de Sousa, Edson Antonio Capello; Rocha, Eduardo Passos

    2009-07-01

    This finite element analysis compared stress distribution on complete dentures and implant-retained overdentures with different attachment systems. Four models of edentulous mandible were constructed: group A (control), complete denture; group B, overdenture retained by 2 splinted implants with bar-clip system; group C, overdenture retained by 2 unsplinted implants with o'ring system; and group D, overdenture retained by 2 splinted implants with bar-clip and 2 distally placed o'ring system. Evaluation was performed on Ansys software, with 100-N vertical load applied on central incisive teeth. The lowest maximum general stress value (in megapascal) was observed in group A (64.305) followed by groups C (119.006), D (258.650), and B (349.873). The same trend occurred in supporting tissues with the highest stress value for cortical bone. Unsplinted implants associated with the o'ring attachment system showed the lowest maximum stress values among all overdenture groups. Furthermore, o'ring system also improved stress distribution when associated with bar-clip system.

  9. [Induction of free radicals after dietary exposure by mercury contaminated rice and protective effect of coexisting selenium].

    PubMed

    Ji, Xiu-ling; Cheng, Jin-ping; Wang, Wen-hua; Qu, Li-ya; Zhao, Xiao-xiang; Zhuang, Hui-sheng

    2006-10-01

    Sprague-Dawley rats were reared by environmental mercury contaminated rice to survey the potential health risk of Wanshan mercury mining area. Electron spin resonance (ESR) was introduced to detect the species and the intensities of free radicals, using spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The results showed that the mercury-contaminated rice significantly increased the levels of free radicals and MDA in rat brain at 7 days (p < 0.05). ESR spectrums showed that the principal spin adducts resulted from the trapping of alkyl free radical (alphaH = 22.7 x 10(-4)T +/- 1.6 x 10(-4)T, alphaN = 15.5 x 10(-4)T +/- 0.5 x 10(-4)T), and hydroxyl radical. Levels of free radicals and MDA increased slowly until after 90-day exposure period (83%, 100%). Element correlation analysis showed high correlations of mercury and selenium in the brain of rat fed with Wanshan rice, suggesting that the coexisting selenium in rice exhibited antagonistic effects on both mercury accumulation and toxicity. The slight increases of free radicals in rat brain at 7, 20 and 30-day exposure periods should be related with the scavenger effect of Se.

  10. Guanosine 2-NH2 groups of Escherichia coli RNase P RNA involved in intramolecular tertiary contacts and direct interactions with tRNA.

    PubMed Central

    Heide, C; Pfeiffer, T; Nolan, J M; Hartmann, R K

    1999-01-01

    We have identified by nucleotide analog interference mapping (NAIM) exocyclic NH2 groups of guanosines in RNase P RNA from Escherichia coli that are important for tRNA binding. The majority of affected guanosines represent phylogenetically conserved nucleotides. Several sites of interference could be assigned to direct contacts with the tRNA moiety, whereas others were interpreted as reflecting indirect effects on tRNA binding due to the disruption of tertiary contacts within the catalytic RNA. Our results support the involvement of the 2-NH2 groups of G292/G293 in pairing with C74 and C75 of tRNA CCA-termini, as well as formation of two consecutive base triples involving C75 and A76 of CCA-ends interacting with G292/A258 and G291/G259, respectively. Moreover, we present first biochemical evidence for two tertiary contacts (L18/P8 and L8/P4) within the catalytic RNA, whose formation has been postulated previously on the basis of phylogenetic comparative analyses. The tRNA binding interference data obtained in this and our previous studies are consistent with the formation of a consecutive nucleotide triple and quadruple between the tetraloop L18 and helix P8. Formation of the nucleotide triple (G316 and A94:U104 in wild-type E. coli RNase P RNA) is also supported by mutational analysis. For the mutant RNase P RNA carrying a G94:C104 double mutation, an additional G316-to-A mutation resulted in a restoration of binding affinity for mature and precursor tRNA. PMID:9917070

  11. Synthesis, interaction with DNA and bovine serum albumin of the transition metal complexes of demethylcantharate and 2-aminobenzothiazole

    NASA Astrophysics Data System (ADS)

    Zhang, Fan; Lin, Qiu-Yue; Li, Shi-Kun; Zhao, Yu-Ling; Wang, Peng-Peng; Chen, Miao-Miao

    2012-12-01

    Four new transition metal complexes (Habtz)2[M(DCA)2]·6H2O (M = Co(II) (1), Ni(II) (2), Cu(II) (3), Zn(II) (4); DCA = demethylcantharate, 7-oxabicyclo [2.2.1]heptane-2,3-dicarboxylate, C8H8O5; Habtz = 2-aminobenzothiazole acid, C7H7N2S) were synthesized and characterized by elemental analysis, molar conductance, infrared spectra and thermogravimetric analysis. The coordination number of complex was six. The X-ray diffraction analysis indicated that complex 3 crystallized in the triclinic crystal system with P1¯ space group. The DNA-binding properties of the complexes were investigated by electronic absorption spectra, fluorescence spectra, viscosity measurements. Title complexes could bind to DNA via partial intercalative mode. The Kb of the complexes were 5.33 × 104 (1), 7.04 × 104 (2), 9.91 × 104 (3) and 5.03 × 104 L mol-1 (4). The results of agarose gel electrophoresis showed that Cu(II) complex could cleave pBR322 plasmid DNA via radical-based mechanism. The complexes could quench the intrinsic fluorescence of bovine serum albumin (BSA) through a static quenching with the binding constants Ka of 1.11 × 104 (1), 1.24 × 106 (2), 8.42 × 105 (3) and 1.75 × 104 L mol-1 (4). The complexes had intense antiproliferative activities against human hepatoma cell lines (SMMC7721) and human gastric cancer cells (MGC80-3) lines in vitro. Cu(II) complex had the strongest activity against human gastric cancer cells.

  12. Identification of a novel AluSx-mediated deletion of exon 3 in the SBDS gene in a patient with Shwachman-Diamond syndrome.

    PubMed

    Costa, Elísio; Duque, Frederico; Oliveira, Jorge; Garcia, Paula; Gonçalves, Isabel; Diogo, Luísa; Santos, Rosário

    2007-01-01

    Shwachman-Diamond syndrome (SDS) is caused by mutations in the SBDS gene, most of which are the result of gene conversion events involving its highly homologous pseudogene SBDSP. Here we describe the molecular characterization of the first documented gross deletion in the SBDS gene, in a 4-year-old Portuguese girl with SDS. The clinical diagnosis was based on the presence of hematological symptoms (severe anemia and cyclic neutropenia), pancreatic exocrine insufficiency and skeletal abnormalities. Routine molecular screening revealed heterozygosity for the common splicing mutation c.258+2T>C, and a further step-wise approach led to the detection of a large deletion encompassing exon 3, the endpoints of which were subsequently delineated at the gDNA level. This novel mutation (c.258+374_459+250del), predictably giving rise to an internally deleted polypeptide (p.Ile87_Gln153del), appears to have arisen from an excision event mediated by AluSx elements which are present in introns 2 and 3. Our case illustrates the importance of including gross deletion screening in the SDS diagnostic setting, especially in cases where only one deleterious mutation is detected by routine screening methods. In particular, deletional rearrangements involving exon 3 should be considered, since Alu sequences are known to be an important cause of recurrent mutations.

  13. Diversity, virulence, and antimicrobial resistance of the KPC-producing Klebsiella pneumoniae ST307 clone

    PubMed Central

    Villa, Laura; Feudi, Claudia; Fortini, Daniela; Brisse, Sylvain; Passet, Virginie; Bonura, Celestino; Endimiani, Andrea; Mammina, Caterina; Ocampo, Ana Maria; Jimenez, Judy Natalia; Doumith, Michel; Woodford, Neil; Hopkins, Katie

    2017-01-01

    The global spread of Klebsiella pneumoniae producing Klebsiella pneumoniae carbapenemase (KPC) has been mainly associated with the dissemination of high-risk clones. In the last decade, hospital outbreaks involving KPC-producing K. pneumoniae have been predominantly attributed to isolates belonging to clonal group (CG) 258. However, results of recent epidemiological analysis indicate that KPC-producing sequence type (ST) 307, is emerging in different parts of the world and is a candidate to become a prevalent high-risk clone in the near future. Here we show that the ST307 genome encodes genetic features that may provide an advantage in adaptation to the hospital environment and the human host. Sequence analysis revealed novel plasmid-located virulence factors, including a cluster for glycogen synthesis. Glycogen production is considered to be one of the possible adaptive responses to long-term survival and growth in environments outside the host. Chromosomally-encoded virulence traits in the clone comprised fimbriae, an integrative conjugative element carrying the yersiniabactin siderophore, and two different capsular loci. Compared with the ST258 clone, capsulated ST307 isolates showed higher resistance to complement-mediated killing. The acquired genetic features identified in the genome of this new emerging clone may contribute to increased persistence of ST307 in the hospital environment and shed light on its potential epidemiological success. PMID:28785421

  14. Outcome, Cost, and Oversight of Iraq Reconstruction Contract W914NS-04-D-0006

    DTIC Science & Technology

    2008-01-28

    Minerals HQs Building $7,928,993 2% 03 Modernize Maternity & Pediatric Hospital – Ibn Al Baladi $8,814,353 3% 04 Ministry of Health – Healthcare...Ministerial buildings 8,257,981 8,258,377 Completed Terminated for Convenience 3 Renovate Ibn Al Baladi Hospital 9,585,023 9,365,516 4 Design and...7,331,585 237,490 688,906 8,257,981 8,258,377 8,258,377 3 Renovate Ibn Al Baladi Hospital 8,251,610 320,820 1,012,593 9,585,023 9,585,023

  15. Differential Th17 response induced by the two clades of the pandemic ST258 Klebsiella pneumoniae clonal lineages producing KPC-type carbapenemase

    PubMed Central

    Clemente, Ann Maria; Castronovo, Giuseppe; Antonelli, Alberto; D’Andrea, Marco Maria; Tanturli, Michele; Perissi, Eloisa; Paccosi, Sara; Parenti, Astrid; Cozzolino, Federico; Rossolini, Gian Maria

    2017-01-01

    The spread of KPC-type carbapenemases is mainly attributed to the global dissemination of Klebsiella pneumoniae (KP) strains belonging to the clonal group (CG) 258, including sequence type (ST) 258 and other related STs. Two distinct clades of CG258-KP have evolved, which differ mainly for the composition of their capsular polysaccharides, and recent studies indicate that clade 1 evolved from an ancestor of clade 2 by recombination of a genomic fragment carrying the capsular polysaccharide (cps) locus. In this paper, we investigated the ability of two ST258-KP strains, KKBO-1 and KK207-1, selected as representatives of ST258-KP clade 2 and clade 1, respectively, to activate an adaptive immune response using ex vivo-stimulation of PBMC from normal donors as an experimental model. Our data showed that KKBO-1 (clade 2) induces a Th17 response more efficiently than KK207-1 (clade 1): the percentage of CD4+IL17+ cells and the production of IL-17A were significantly higher in cultures with KKBO-1 compared to cultures with KK207-1. While no differences in the rate of bacterial internalization or in the bacteria-induced expression of CD86 and HLA-DR by monocytes and myeloid dendritic cells were revealed, we found that the two strains significantly differ in inducing the production of cytokines involved in the adaptive immune response, as IL-1β, IL-23 and TNF-α, by antigen-presenting cells, with KKBO-1 being a more efficient inducer than KK207-1. The immune responses elicited by KK207-1 were comparable to those elicited by CIP 52.145, a highly virulent K. pneumoniae reference strain known to escape immune-inflammatory responses. Altogether, present results suggest that CG258-KP of the two clades are capable of inducing a different response of adaptive immunity in the human host. PMID:28586386

  16. Development of Enhanced Avionics Flight Hardware Selection Process

    NASA Technical Reports Server (NTRS)

    Smith, K.; Watson, G. L.

    2003-01-01

    The primary objective of this research was to determine the processes and feasibility of using commercial off-the-shelf PC104 hardware for flight applications. This would lead to a faster, better, and cheaper approach to low-budget programs as opposed to the design, procurement. and fabrication of space flight hardware. This effort will provide experimental evaluation with results of flight environmental testing. Also, a method and/or suggestion used to bring test hardware up to flight standards will be given. Several microgravity programs, such as the Equiaxed Dendritic Solidification Experiment, Self-Diffusion in Liquid Elements, and various other programs, are interested in PC104 environmental testing to establish the limits of this technology.

  17. 40 CFR 258.28 - Liquids restrictions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... septic waste; (2) The waste is leachate or gas condensate derived from the MSWLF unit and the MSWLF unit... leachate collection system as described in § 258.40(a)(2) of this part. The owner or operator must place...

  18. 40 CFR 258.28 - Liquids restrictions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... septic waste; (2) The waste is leachate or gas condensate derived from the MSWLF unit and the MSWLF unit... leachate collection system as described in § 258.40(a)(2) of this part. The owner or operator must place...

  19. 40 CFR 258.28 - Liquids restrictions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... septic waste; (2) The waste is leachate or gas condensate derived from the MSWLF unit and the MSWLF unit... leachate collection system as described in § 258.40(a)(2) of this part. The owner or operator must place...

  20. Uncertainties in nuclear transition matrix elements for neutrinoless {beta}{beta} decay

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rath, P. K.

    Uncertainties in nuclear transition matrix elements M{sup (0{nu})} and M{sub N}{sup (0{nu})} due to the exchange of light and heavy Majorana neutrinos, respectively have been estimated by calculating sets of twelve nuclear transition matrix elements for the neutrinoless {beta}{beta} decay of {sup 94,96}Zr, {sup 98,100}Mo, {sup 104}Ru, {sup 110}Pd, {sup 128,130}Te and {sup 150}Nd isotopes in the case of 0{sup +}{yields}0{sup +} transition by considering four different parameterizations of a Hamiltonian with pairing plus multipolar effective two-body interaction and three different parameterizations of Jastrow short range correlations. Exclusion of nuclear transition matrix elements calculated with the Miller-Spencer parametrization reduces themore » uncertainties by 10%-15%.« less

  1. 258. Dennis Hill, Photographer April 1998 VIEW OF CANTILEVER TRUSS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    258. Dennis Hill, Photographer April 1998 VIEW OF CANTILEVER TRUSS ANCHOR ARM AT PIERS E- AND E-2, SOUTH SIDE, FACING NORTH. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

  2. 40 CFR 258.25 - Access requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... wastes by using artificial barriers, natural barriers, or both, as appropriate to protect human health....25 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.25 Access requirements. Owners or operators of all...

  3. 40 CFR 258.12 - Wetlands.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.12 Wetlands. (a) New MSWLF units and lateral...) Impacts on fish, wildlife, and other aquatic resources and their habitat from release of the solid waste...

  4. 40 CFR 258.12 - Wetlands.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.12 Wetlands. (a) New MSWLF units and lateral...) Impacts on fish, wildlife, and other aquatic resources and their habitat from release of the solid waste...

  5. Effect of Heat Treatment on Chemical Segregation in CMSX-4 Nickel-Base Superalloy

    NASA Astrophysics Data System (ADS)

    Szczotok, A.; Chmiela, B.

    2014-08-01

    Superalloys display a strong tendency toward chemical segregation during solidification. Therefore, it is of great importance to develop appropriate techniques for the melting and casting of superalloys. Elements partitioning between the γ and γ' phases in single crystal superalloys have been investigated by several authors using electron probe microanalysis (Hemmersmeier and Feller-Kniepmeier Mater Sci Eng A 248:87-97, 1998; Kearsey et al. Intermetallics 12:903-910, 2004; Kearsey et al. Superalloys 2004, pp 801-810, 2004; D'Souza et al. Mater Sci Eng A 490:258-265, 2008). We examined the effect of the particular stages of standard heat treatment (solution treatment and ageing) applied to CMSX-4 single crystal superalloy on chemical segregation that occurs between dendrites and interdendritic areas. Dendritic structures were observed using a scanning electron microscope. Analyses of the chemical composition were performed using energy dispersive x-ray spectroscopy. The obtained qualitative and quantitative results for the concentrations of elements enabled us to confirm the dendritic segregation in as-cast CMSX-4 superalloy. The concentrations of some refractory elements (tungsten, rhenium) were much greater in dendrites than in interdendritic areas. However, these differences in chemical composition gradually decreased during heat treatment. The results obtained in this study warrant further examination of the diffusion processes of elements during heat treatment of the investigated superalloy, and of the kinetics of diffusion.

  6. Effects of lead, molybdenum, rubidium, arsenic and organochlorines on spermatogenesis in fish: monitoring at Mekong Delta area and in vitro experiment.

    PubMed

    Yamaguchi, Sonoko; Miura, Chiemi; Ito, Aki; Agusa, Tetsuro; Iwata, Hisato; Tanabe, Shinsuke; Tuyen, Bui Cach; Miura, Takeshi

    2007-06-05

    To estimate the influence of water contaminants on fish reproduction in the Mekong Delta area, we sampled cultivated male catfish (Pangasianodon hypophthalmus), investigated testicular development, and measured persistent organic pollutants (POPs) and trace element levels in muscle and liver, respectively. Various testes sizes were observed although sampling took place during a short period. Histological analysis revealed that all developmental stages of germ cells were observed in catfish with large testis, whereas only necrotic spermatogonia but no other germ cells were observed in catfish with small testis. In small testis, furthermore, vacuolization and hypertrophy of Sertoli cells were observed. Measurement of POPs in muscle and trace elements in liver demonstrated that there were negative correlations between GSI and the concentrations of Pb, Mo, Rb and As. To clarify possible direct effects of Pb, Mo, Rb and As on spermatogenesis in fish, we investigated the effects of these trace elements on spermatogenesis using in vitro testicular organ culture of Japanese eel (Anguilla japonica). Treatment with each of the trace elements alone did not affect spermatogenesis. However, treatment with 10(-7)M of Pb, 10(-5) and 10(-4)M of Mo, 10(-5)-10(-3)M of Rb or 10(-5)M of As inhibited the spermatogenesis induced by 11-ketotestosterone (11KT). Furthermore, treatment with 10(-4)M of As in combination with 11KT caused necrosis of testicular fragments. Taken together, these results are consistent with the hypothesis that Pb, Mo, Rb and As can exert inhibitory effects on spermatogenesis in catfish inhabiting the Mekong Delta area.

  7. 40 CFR 258.24 - Air criteria.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.24 Air criteria. (a) Owners or operators of all MSWLFs... Act, as amended. (b) Open burning of solid waste, except for the infrequent burning of agricultural...

  8. 40 CFR 258.24 - Air criteria.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Operating Criteria § 258.24 Air criteria. (a) Owners or operators of all MSWLFs... Act, as amended. (b) Open burning of solid waste, except for the infrequent burning of agricultural...

  9. Interaction with biomacromolecules and antiproliferative activities of Mn(II), Ni(II), Zn(II) complexes of demethylcantharate and 2,2'-bipyridine

    NASA Astrophysics Data System (ADS)

    Zhang, Fan; Lin, Qiu-Yue; Hu, Wan-Li; Song, Wen-Ji; Shen, Shu-Ting; Gui, Pan

    2013-06-01

    Three new transition metal complexes [Mn2(DCA)2(bipy)2]·5H2O (1), [M2(DCA)2(bipy)2(H2O)]·10H2O(M = Ni(II)(2);Zn(II)(3)), (DCA = demethylcantharate, 7-oxabicyclo[2,2,1]heptane-2,3-dicarboxylate, C8H8O5) were synthesized and characterized by elemental analysis, molar conductance, infrared spectra and X-ray diffraction techniques. Each metal ion was six-coordinated in complexes. Complex 1 has a Mn2O2 center. Complexes 2 and 3 have asymmetric binuclear structure. Great amount of intermolecular hydrogen-bonding and π-π* stacking interactions were formed in these complex structures. The DNA-binding properties of complexes were investigated by electronic absorption spectra and viscosity measurements. The DNA binding constants Kb/(L mol-1) were 1.71 × 104 (1), 2.62 × 104 (2) and 1.59 × 104 (3) at 298 K. The complexes could quench the intrinsic fluorescence of bovine serum albumin (BSA) strongly through static quenching. The protein binding constants Ka/(L mol-1) were 7.27 × 104 (1), 4.55 × 104 (2) and 7.87 × 104 L mol-1 (3) and binding site was one. The complexes bind more tightly with DNA and BSA than with ligands. Complexes 1 and 3 had stronger inhibition ratios than Na2(DCA) against human hepatoma cells (SMMC-7721) lines and human gastric cancer cells (MGC80-3) lines in vitro. Complex 3 showed the strongest antiproliferative activity against SMMC-7721 (IC50 = 29.46 ± 2.12 μmol L-1) and MGC80-3 (IC50 = 27.02 ± 2.38 μmol L-1), which shows potential in anti-cancer drug development.

  10. The Joint Airlock Module is moved to the payload canister

    NASA Technical Reports Server (NTRS)

    2001-01-01

    KENNEDY SPACE CENTER, Fla. -- In the Space Station Processing Facility, the Joint Airlock Module is moved closer to the payload canister. The airlock will be installed in the payload bay of Atlantis for mission STS-104 to the International Space Station. The airlock is a pressurized flight element consisting of two cylindrical chambers attached end-to-end by a connecting bulkhead and hatch. Once installed and activated, the Airlock becomes the primary path for spacewalk entry to and departure from the Space Station for U.S. spacesuits, which are known as Extravehicular Mobility Units, or EMUs. In addition, the Joint Airlock is designed to support the Russian Orlan spacesuit for EVA activity. STS-104 is scheduled for launch June 14 from Launch Pad 39B.

  11. Research on Electromagnetic Force Distribution and Vibration Performance of A Novel 10/4 Switched Reluctance Motor

    NASA Astrophysics Data System (ADS)

    Fu, Ziyu; Wang, Xinyu; Cao, Cheng; Liu, Meng; Wang, Kangxi

    2017-06-01

    Radial electromagnetic force is one of the main reasons causing the vibration and noise of the switched reluctance motor. Based on this, the novel structure of 10/4 pole switched reluctance motor is proposed, which increases the air gap flux and electromagnetic torque by increasing the number of stator poles. In addition, the excitation current of the stator winding is reduced by early turn-off angle. Through the finite element modelling analysis, the results show the superiority of the new type of switched reluctance motor. In the end, the vibration characteristics of the conventional motor and the new motor are compared and analysed, and the effect of the structure of this new type of switched reluctance motor is verified.

  12. 50 CFR 216.258 - Renewal of Letters of Authorization.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE MAMMALS Taking Marine Mammals Incidental to Conducting Precision Strike Weapon Missions in the Gulf of Mexico § 216.258 Renewal of Letters of Authorization. (a) A Letter of Authorization...

  13. 40 CFR 258.54 - Detection monitoring program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.54 Detection... reaction products in the unsaturated zone beneath the MSWLF unit; (iii) The detectability of indicator parameters, waste constituents, and reaction products in the ground water; and (iv) The concentration or...

  14. Silica dust, diesel exhaust, and painting work are the significant occupational risk factors for lung cancer in nonsmoking Chinese men.

    PubMed

    Tse, L A; Yu, It-S; Au, J S K; Qiu, H; Wang, X-R

    2011-01-04

    Few epidemiological studies have explored the associations between occupational exposures and lung cancer in lifelong nonsmoking men. We obtained lifetime occupational history and other relevant information for 132 newly diagnosed lung cancer cases among nonsmoking Chinese men and 536 nonsmoking community referents. Unconditional multiple logistic regression analysis was performed to estimate the odds ratio (OR) of lung cancer for specific occupational exposures. Significantly increased lung cancer risk was found for nonsmoking workers occupationally exposed to silica dust (OR=2.58, 95% confidence interval (CI): 1.11, 6.01), diesel exhaust (OR=3.47, 95% CI: 1.08, 11.14), spray painting (OR=2.81, 95% CI: 1.14, 6.93), and nonspray painting work (OR=2.36, 95% CI: 1.04, 5.37). Silica dust exposure was associated with a significantly increased risk of adenocarcinoma (OR=2.91, 95% CI: 1.10, 7.68). We observed a positive gradient of all lung cancers and of adenocarcinoma with duration of employment for workers exposed to silica dust and spray painting. This study found an increased risk of lung cancer among nonsmoking Chinese men occupationally exposed to silica dust, diesel exhaust, and painting work.

  15. 75 FR 258 - Airworthiness Directives; Bombardier, Inc. (Type Certificate Previously Held by Bombardier...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-05

    ....; Canadair) Model CL-600- 2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY: Federal Aviation... the AD docket. List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety..., January 5, 2010 / Proposed Rules#0;#0; [[Page 258

  16. 40 CFR 258.4 - Research, development, and demonstration permits.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General § 258.4 Research, development, and... include such terms and conditions at least as protective as the criteria for municipal solid waste... and quantities of municipal solid waste and non-hazardous wastes which the State Director deems...

  17. 40 CFR 258.4 - Research, development, and demonstration permits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General § 258.4 Research, development, and... include such terms and conditions at least as protective as the criteria for municipal solid waste... and quantities of municipal solid waste and non-hazardous wastes which the State Director deems...

  18. 7 CFR 1924.258 - Notification of borrowers.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.258... frames for filing claims under this subpart. FmHA or its successor agency under Public Law 103-354 will also notify and advise borrowers of the construction defects procedure at any time construction defects...

  19. 7 CFR 1924.258 - Notification of borrowers.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.258... frames for filing claims under this subpart. FmHA or its successor agency under Public Law 103-354 will also notify and advise borrowers of the construction defects procedure at any time construction defects...

  20. 7 CFR 1924.258 - Notification of borrowers.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.258... frames for filing claims under this subpart. FmHA or its successor agency under Public Law 103-354 will also notify and advise borrowers of the construction defects procedure at any time construction defects...

  1. 7 CFR 1924.258 - Notification of borrowers.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.258... frames for filing claims under this subpart. FmHA or its successor agency under Public Law 103-354 will also notify and advise borrowers of the construction defects procedure at any time construction defects...

  2. 7 CFR 1924.258 - Notification of borrowers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.258... frames for filing claims under this subpart. FmHA or its successor agency under Public Law 103-354 will also notify and advise borrowers of the construction defects procedure at any time construction defects...

  3. 75 FR 12536 - City of Tenakee Springs; Notice of Declaration of Intention and Soliciting Comments, Protests...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    .... 47 S, R. 63 E, secs. 15, 21, and 22, Copper River Meridian. g. Filed Pursuant to: Section 23(b)(1) of..., Anchorage, AK 99503; telephone: (907) 258-2420; FAX: (907) 258-2419; e- mail: [email protected] . i...

  4. GRS 1758-258: Into the thermal dominant state with Swift XRT

    NASA Astrophysics Data System (ADS)

    Hirsch, M.; Pottschmidt, K.; Krauss, F.; Eikmann, W.; Kreykenbohm, I.; Wilms, J.; Kuehnel, M.; Rodrigues, B. H. G.; Soria, R.; Grinberg, V.; Smith, D. M.; Bel, M. Cadolle; Tomsick, J. A.; Bodaghee, A.; Kuulkers, E.; INTEGRAL Galactic Bulge Monitoring Team; Kalemci, E.; Miller, J. M.

    2016-12-01

    A decline of the hard flux of the Galactic black hole candidate GRS 1758-258 was observed with Swift BAT and INTEGRAL ISGRI starting in 2016 mid-August, with the source having become undetectable above 20 keV around 2016 September 30 (ATEL #9625).

  5. Trace elements in sera from patients with renal disease

    NASA Astrophysics Data System (ADS)

    Miura, Yoshinori; Nakai, Keiko; Sera, Kouichiro; Sato, Michirou

    1999-04-01

    In hemodialysis (HD) patients, an accumulation of trace elements such as aluminum, copper, silicon and vanadium has been reported. Aluminum-caused encephalopathy and aluminum-related bone diseases are important trace element-related complications. Using particle induced X-ray emission (PIXE) we determined concentrations of aluminum, silicon, copper, zinc, selenium and bromine in sera of 29 patients with HD, 14 nondialysis patients with renal disease (RD) and 27 normal controls. The concentration of serum silicon of the patients with HD was 107.4 ± 61.3 μmol/l, which is markedly higher than that of normal controls (48.3 ± 25.8 μmol/l, p < 0.0001). The serum concentrations of zinc and bromine in patients with HD were 11.9 ± 1.7 and 21.3 ± 3.0 μmol/l, respectively. Both were markedly lower than those of normal controls (15.6 ± 2.6, 69.2 ± 8.3 μmol/l, p < 0.0001). The concentrations of aluminium and bromine in the serum of patients with RD were 171.9 ± 64.3 and 81.9 ± 11.6 μmol/l, which were markedly higher than those of normal controls ( p < 0.0001, p < 0.001). No significant differences were observed in the concentration of copper and selenium among three groups.

  6. 24 CFR 92.258 - Elder cottage housing opportunity (ECHO) units.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Housing and Urban Development HOME INVESTMENT PARTNERSHIPS PROGRAM Project Requirements § 92.258 Elder cottage housing opportunity (ECHO) units. (a) General. HOME funds may be used for the initial purchase and... designed to be installed adjacent to existing single-family dwellings. (b) Eligible owners. The owner of a...

  7. 40 CFR 258.56 - Assessment of corrective measures.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Assessment of corrective measures. 258... Assessment of corrective measures. (a) Within 90 days of finding that any of the constituents listed in... assessment of corrective measures. Such an assessment must be completed within a reasonable period of time...

  8. 40 CFR 258.56 - Assessment of corrective measures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Assessment of corrective measures. 258... Assessment of corrective measures. (a) Within 90 days of finding that any of the constituents listed in... assessment of corrective measures. Such an assessment must be completed within a reasonable period of time...

  9. 40 CFR 258.56 - Assessment of corrective measures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Assessment of corrective measures. 258... Assessment of corrective measures. (a) Within 90 days of finding that any of the constituents listed in... assessment of corrective measures. Such an assessment must be completed within a reasonable period of time...

  10. 40 CFR 258.61 - Post-closure care requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the final cover; (2) Maintaining and operating the leachate collection system in accordance with the...) Maintaining and operating the gas monitoring system in accordance with the requirements of § 258.23. (b) The... stop managing leachate if the owner or operator demonstrates that leachate no longer poses a threat to...

  11. 40 CFR 258.3 - Consideration of other Federal laws.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Consideration of other Federal laws... CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General § 258.3 Consideration of other Federal laws. The owner or operator of a municipal solid waste landfill unit must comply with any other applicable Federal...

  12. 40 CFR 258.3 - Consideration of other Federal laws.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Consideration of other Federal laws... CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General § 258.3 Consideration of other Federal laws. The owner or operator of a municipal solid waste landfill unit must comply with any other applicable Federal...

  13. 40 CFR 258.3 - Consideration of other Federal laws.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Consideration of other Federal laws... CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS General § 258.3 Consideration of other Federal laws. The owner or operator of a municipal solid waste landfill unit must comply with any other applicable Federal...

  14. 8 CFR 258.3 - Action upon arrival.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS LIMITATIONS ON PERFORMANCE OF LONGSHORE WORK BY ALIEN CREWMEN § 258.3 Action upon arrival. (a) The master or agent of the vessel... the vessel will perform longshore work at any port before departing the United States; and (2) If...

  15. 40 CFR 258.71 - Financial assurance for closure.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....71 Section 258.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... closure. (a) The owner or operator must have a detailed written estimate, in current dollars, of the cost.... (1) The cost estimate must equal the cost of closing the largest area of all MSWLF unit ever...

  16. 40 CFR 258.71 - Financial assurance for closure.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....71 Section 258.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... closure. (a) The owner or operator must have a detailed written estimate, in current dollars, of the cost.... (1) The cost estimate must equal the cost of closing the largest area of all MSWLF unit ever...

  17. 40 CFR 258.16 - Closure of existing municipal solid waste landfill units.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.16 Closure of existing municipal solid waste landfill units. (a) Existing MSWLF units that cannot make the... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Closure of existing municipal solid...

  18. 40 CFR 258.16 - Closure of existing municipal solid waste landfill units.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Location Restrictions § 258.16 Closure of existing municipal solid waste landfill units. (a) Existing MSWLF units that cannot make the... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Closure of existing municipal solid...

  19. RCRA SUBTITLE D (258): SEISMIC DESIGN GUIDANCE FOR MUNICIPAL SOLID WASTE LANDFILL FACILITIES

    EPA Science Inventory

    On October 9, 1993, the new RCRA Subtitle D regulations (40 CFR Part 258) went into effect. These regulations are applicable to landfills receiving municipal solid waste (MSW) and establish minimum Federal criteria for the siting, design, operation, and closure of MSW landfills....

  20. 40 CFR 258.54 - Detection monitoring program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.54 Detection... indicator parameters for a MSWLF unit, in lieu of some or all of the heavy metals (constituents 1-15 in... parameters, waste constituents, and reaction products in the ground water; and (iv) The concentration or...

  1. 40 CFR 258.54 - Detection monitoring program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.54 Detection... indicator parameters for a MSWLF unit, in lieu of some or all of the heavy metals (constituents 1-15 in... parameters, waste constituents, and reaction products in the ground water; and (iv) The concentration or...

  2. 40 CFR 258.54 - Detection monitoring program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.54 Detection... indicator parameters for a MSWLF unit, in lieu of some or all of the heavy metals (constituents 1-15 in... parameters, waste constituents, and reaction products in the ground water; and (iv) The concentration or...

  3. 40 CFR 258.54 - Detection monitoring program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective Action § 258.54 Detection... indicator parameters for a MSWLF unit, in lieu of some or all of the heavy metals (constituents 1-15 in... parameters, waste constituents, and reaction products in the ground water; and (iv) The concentration or...

  4. 40 CFR 258.58 - Implementation of the corrective action program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective...) Establish and implement a corrective action ground-water monitoring program that: (i) At a minimum, meet the requirements of an assessment monitoring program under § 258.55; (ii) Indicate the effectiveness of the...

  5. 40 CFR 258.58 - Implementation of the corrective action program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective...) Establish and implement a corrective action ground-water monitoring program that: (i) At a minimum, meet the requirements of an assessment monitoring program under § 258.55; (ii) Indicate the effectiveness of the...

  6. 40 CFR 258.58 - Implementation of the corrective action program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Ground-Water Monitoring and Corrective...) Establish and implement a corrective action ground-water monitoring program that: (i) At a minimum, meet the requirements of an assessment monitoring program under § 258.55; (ii) Indicate the effectiveness of the...

  7. 40 CFR 239.6 - Permitting requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... CFR part 257, subpart B or 40 CFR part 258 federal revised criteria. (e) For municipal solid waste... solid waste landfill units shall have a permit incorporating the conditions identified in paragraph (e... solid waste landfill units which achieve compliance with 40 CFR part 258, subpart E; (vi) Closure and...

  8. 40 CFR 239.6 - Permitting requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... CFR part 257, subpart B or 40 CFR part 258 federal revised criteria. (e) For municipal solid waste... solid waste landfill units shall have a permit incorporating the conditions identified in paragraph (e... solid waste landfill units which achieve compliance with 40 CFR part 258, subpart E; (vi) Closure and...

  9. 40 CFR 239.6 - Permitting requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CFR part 257, subpart B or 40 CFR part 258 federal revised criteria. (e) For municipal solid waste... solid waste landfill units shall have a permit incorporating the conditions identified in paragraph (e... solid waste landfill units which achieve compliance with 40 CFR part 258, subpart E; (vi) Closure and...

  10. 40 CFR 258.50 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... in § 258.2) during the active life of the unit and the post-closure care period. This demonstration... shall be conducted throughout the active life and post-closure care period of that MSWLF unit as... scientist or engineer who has received a baccalaureate or post-graduate degree in the natural sciences or...

  11. 40 CFR 258.50 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... in § 258.2) during the active life of the unit and the post-closure care period. This demonstration... shall be conducted throughout the active life and post-closure care period of that MSWLF unit as... scientist or engineer who has received a baccalaureate or post-graduate degree in the natural sciences or...

  12. 40 CFR 258.50 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... in § 258.2) during the active life of the unit and the post-closure care period. This demonstration... shall be conducted throughout the active life and post-closure care period of that MSWLF unit as... scientist or engineer who has received a baccalaureate or post-graduate degree in the natural sciences or...

  13. 40 CFR 258.50 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... in § 258.2) during the active life of the unit and the post-closure care period. This demonstration... shall be conducted throughout the active life and post-closure care period of that MSWLF unit as... scientist or engineer who has received a baccalaureate or post-graduate degree in the natural sciences or...

  14. 8 CFR 210.2 - Application for temporary resident status.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... years or older, the application must be accompanied by a completed Form FD-258 (Fingerprint Card). (ii... applicant shall proceed to a legalization office for presentation or completion of Form FD-258 (Fingerprint... certificate accompanied by additional evidence of identity bearing a photograph and/or fingerprint of the...

  15. 8 CFR 210.2 - Application for temporary resident status.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... years or older, the application must be accompanied by a completed Form FD-258 (Fingerprint Card). (ii... applicant shall proceed to a legalization office for presentation or completion of Form FD-258 (Fingerprint... certificate accompanied by additional evidence of identity bearing a photograph and/or fingerprint of the...

  16. 8 CFR 210.2 - Application for temporary resident status.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... years or older, the application must be accompanied by a completed Form FD-258 (Fingerprint Card). (ii... applicant shall proceed to a legalization office for presentation or completion of Form FD-258 (Fingerprint... certificate accompanied by additional evidence of identity bearing a photograph and/or fingerprint of the...

  17. 40 CFR 258.51 - Ground-water monitoring systems.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the uppermost aquifer (as defined in § 258.2) that: (1) Represent the quality of background ground water that has not been affected by leakage from a unit. A determination of background quality may...; or (ii) Sampling at other wells will provide an indication of background ground-water quality that is...

  18. Ellipsoidal terrain correction based on multi-cylindrical equal-area map projection of the reference ellipsoid

    NASA Astrophysics Data System (ADS)

    Ardalan, A. A.; Safari, A.

    2004-09-01

    An operational algorithm for computation of terrain correction (or local gravity field modeling) based on application of closed-form solution of the Newton integral in terms of Cartesian coordinates in multi-cylindrical equal-area map projection of the reference ellipsoid is presented. Multi-cylindrical equal-area map projection of the reference ellipsoid has been derived and is described in detail for the first time. Ellipsoidal mass elements with various sizes on the surface of the reference ellipsoid are selected and the gravitational potential and vector of gravitational intensity (i.e. gravitational acceleration) of the mass elements are computed via numerical solution of the Newton integral in terms of geodetic coordinates {λ,ϕ,h}. Four base- edge points of the ellipsoidal mass elements are transformed into a multi-cylindrical equal-area map projection surface to build Cartesian mass elements by associating the height of the corresponding ellipsoidal mass elements to the transformed area elements. Using the closed-form solution of the Newton integral in terms of Cartesian coordinates, the gravitational potential and vector of gravitational intensity of the transformed Cartesian mass elements are computed and compared with those of the numerical solution of the Newton integral for the ellipsoidal mass elements in terms of geodetic coordinates. Numerical tests indicate that the difference between the two computations, i.e. numerical solution of the Newton integral for ellipsoidal mass elements in terms of geodetic coordinates and closed-form solution of the Newton integral in terms of Cartesian coordinates, in a multi-cylindrical equal-area map projection, is less than 1.6×10-8 m2/s2 for a mass element with a cross section area of 10×10 m and a height of 10,000 m. For a mass element with a cross section area of 1×1 km and a height of 10,000 m the difference is less than 1.5×10-4m2/s2. Since 1.5× 10-4 m2/s2 is equivalent to 1.5×10-5m in the vertical direction, it can be concluded that a method for terrain correction (or local gravity field modeling) based on closed-form solution of the Newton integral in terms of Cartesian coordinates of a multi-cylindrical equal-area map projection of the reference ellipsoid has been developed which has the accuracy of terrain correction (or local gravity field modeling) based on the Newton integral in terms of ellipsoidal coordinates.

  19. Failure Behavior Characterization of Mo-Modified Ti Surface by Impact Test and Finite Element Analysis

    NASA Astrophysics Data System (ADS)

    Ma, Yong; Qin, Jianfeng; Zhang, Xiangyu; Lin, Naiming; Huang, Xiaobo; Tang, Bin

    2015-07-01

    Using the impact test and finite element simulation, the failure behavior of the Mo-modified layer on pure Ti was investigated. In the impact test, four loads of 100, 300, 500, and 700 N and 104 impacts were adopted. The three-dimensional residual impact dents were examined using an optical microscope (Olympus-DSX500i), indicating that the impact resistance of the Ti surface was improved. Two failure modes cohesive and wearing were elucidated by electron backscatter diffraction and energy-dispersive spectrometer performed in a field-emission scanning electron microscope. Through finite element forward analysis performed at a typical impact load of 300 N, stress-strain distributions in the Mo-modified Ti were quantitatively determined. In addition, the failure behavior of the Mo-modified layer was determined and an ideal failure model was proposed for high-load impact, based on the experimental and finite element forward analysis results.

  20. Methyl 3-O-α-l-fucopyranosyl β-d-glucopyran­oside tetra­hydrate

    PubMed Central

    Eriksson, Lars; Widmalm, Göran

    2012-01-01

    The title compound, C13H24O10·4H2O, is the methyl glycoside of a disaccharide structural element present in the backbone of the capsular polysaccharide from Klebsiella K1, which contains only three sugars and a substituent in the polysaccharide repeating unit. The conformation of the title disaccharide is described by the glycosidic torsion angles ϕH = 51.1 (1)° and ψH = 25.8 (1)°. In the crystal, a number of O—H⋯O hydrogen bonds link the methyl glycoside and water mol­ecules, forming a three-dimensional network. One water mol­ecule is disordered over two positions with occupancies of 0.748 (4) and 0.252 (4). PMID:23284493

  1. 40 CFR 258.42 - Approval of site-specific flexibility requests in Indian country.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Design Criteria § 258.42 Approval of...) of this section applies to the Salt River Landfill, a municipal solid waste landfill owned and operated by the SPRMIC on the SRPMIC's reservation in Arizona, which includes waste disposal areas...

  2. 40 CFR 258.42 - Approval of site-specific flexibility requests in Indian country.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) SOLID WASTES CRITERIA FOR MUNICIPAL SOLID WASTE LANDFILLS Design Criteria § 258.42 Approval of...) of this section applies to the Salt River Landfill, a municipal solid waste landfill owned and operated by the SPRMIC on the SRPMIC's reservation in Arizona, which includes waste disposal areas...

  3. Performance Characteristics of Automotive Engines in the United States : First Series - Report No. 13 - 1975 American Motors 258 CID (4.2 Liters)

    DOT National Transportation Integrated Search

    1978-04-01

    Experimental data were obtained in dynamometer tests of a 1975 AMC 258-CID production 1V engine to determine fuel consumption and emissions (hydrocarbon, carbon monoxide, oxides of nitrogen) at steady-state engine-operating modes. The objective of th...

  4. 77 FR 2317 - Captain John Smith Chesapeake National Historic Trail Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-17

    ... 1, 2012 via email at [email protected] or telephone: (757) 258-8914; a written summary of... provide public comment should register prior to February 1, 2012 via email at [email protected] or telephone: (757) 258-8914. For those wishing to make comments, please provide a written summary of your...

  5. The Joint Airlock Module is moved to the payload canister

    NASA Technical Reports Server (NTRS)

    2001-01-01

    KENNEDY SPACE CENTER, Fla. -- In the Space Station Processing Facility, workers standing inside the payload canister help guide the Joint Airlock Module into place. The airlock will be installed in the payload bay of Atlantis for mission STS-104 to the International Space Station. The airlock is a pressurized flight element consisting of two cylindrical chambers attached end-to-end by a connecting bulkhead and hatch. Once installed and activated, the Airlock becomes the primary path for spacewalk entry to and departure from the Space Station for U.S. spacesuits, which are known as Extravehicular Mobility Units, or EMUs. In addition, the Joint Airlock is designed to support the Russian Orlan spacesuit for EVA activity. STS-104 is scheduled for launch June 14 from Launch Pad 39B.

  6. The Joint Airlock Module is moved to the payload canister

    NASA Technical Reports Server (NTRS)

    2001-01-01

    KENNEDY SPACE CENTER, Fla. -- In the Space Station Processing Facility, the Joint Airlock Module is lifted from its workstand for a transfer to the payload canister. The airlock will be installed in the payload bay of Atlantis for mission STS-104 to the International Space Station. The airlock is a pressurized flight element consisting of two cylindrical chambers attached end-to-end by a connecting bulkhead and hatch. Once installed and activated, the airlock becomes the primary path for spacewalk entry to and departure from the Space Station for U.S. spacesuits, which are known as Extravehicular Mobility Units, or EMUs. In addition, the Joint Airlock is designed to support the Russian Orlan spacesuit for EVA activity. STS-104 is scheduled for launch June 14 from Launch Pad 39B.

  7. The Role of Epidemic Resistance Plasmids and International High-Risk Clones in the Spread of Multidrug-Resistant Enterobacteriaceae

    PubMed Central

    Mathers, Amy J.; Peirano, Gisele

    2015-01-01

    SUMMARY Escherichia coli sequence type 131 (ST131) and Klebsiella pneumoniae ST258 emerged in the 2000s as important human pathogens, have spread extensively throughout the world, and are responsible for the rapid increase in antimicrobial resistance among E. coli and K. pneumoniae strains, respectively. E. coli ST131 causes extraintestinal infections and is often fluoroquinolone resistant and associated with extended-spectrum β-lactamase production, especially CTX-M-15. K. pneumoniae ST258 causes urinary and respiratory tract infections and is associated with carbapenemases, most often KPC-2 and KPC-3. The most prevalent lineage within ST131 is named fimH30 because it contains the H30 variant of the type 1 fimbrial adhesin gene, and recent molecular studies have demonstrated that this lineage emerged in the early 2000s and was then followed by the rapid expansion of its sublineages H30-R and H30-Rx. K. pneumoniae ST258 comprises 2 distinct lineages, namely clade I and clade II. Moreover, it seems that ST258 is a hybrid clone that was created by a large recombination event between ST11 and ST442. Epidemic plasmids with blaCTX-M and blaKPC belonging to incompatibility group F have contributed significantly to the success of these clones. E. coli ST131 and K. pneumoniae ST258 are the quintessential examples of international multidrug-resistant high-risk clones. PMID:25926236

  8. [Comparative study on five pretreatment methods for ICP-OES determination of mineral elements in Rosa rugosa].

    PubMed

    Zhao, Ning; Yang, Bin; Duan, Yu-Cen; Lei, Ran

    2011-08-01

    Abstract Five different pretreatment methods, including dry ashing and microwave digestion with four acid systems (HNO3 + H2O2, HNO3 + H2O2 + HF, HNO3 + HClO4 and HNO3 + HClO4 + HF), were employed for digestion of the samples of Rosa rugosa. Nine major and trace elements were determined using ICP-OES method. The addition standard recovery rates indicate that, with the system of HNO3 + HClO4, better results can be obtained for most of the determined elements. With this method, except for Fe, the recovery rates are in the range of 95.4%-104.6%. For Fe, higher recovery rate (99%) was obtained with HNO3 + H2O2 + HF system.

  9. Low completion rate of hepatitis B vaccination in female sex workers.

    PubMed

    Magalhães, Rosilane de Lima Brito; Teles, Sheila Araújo; Reis, Renata Karina; Galvão, Marli Teresinha Gimeniz; Gir, Elucir

    2017-01-01

    to assess predictive factors for noncompletion of the hepatitis B vaccination schedule in female sex workers in the city of Teresina, Northeastern Brazil. 402 women were interviewed and, for those who did not wish to visit specialized sites, or did not know their hepatitis B vaccination status, the vaccine was offered at their workplaces. Bi- and multivariate analyses were performed to identify potential predictors for noncompletion of the vaccination schedule. of the 284 women eligible for vaccination, 258 (90.8%) received the second dose, 157/258 (60.8%) and 68/258 (26.3%) received the second and third doses, respectively. Working at clubs and consuming illicit drugs were predictors for noncompletion of the vaccination schedule. the high acceptability of the vaccine's first dose, associated with low completion rates of the vaccination schedule in sex workers, shows the need for more persuasive strategies that go beyond offering the vaccine at their workplaces. avaliar fatores preditores de não completude do esquema vacinal contra hepatite B em mulheres que se prostituem em Teresina, Nordeste do Brasil. Um total de 402 mulheres foi entrevistado e, para as que se negaram a irem a lugares especializados, ou desconheciam sua situação vacinal contra hepatite B, a vacina foi oferecida no local do trabalho. Análises bi e multivariadas foram realizadas para identificar potenciais preditores de não completude do esquema vacinal. Das 284 mulheres elegíveis para vacinação, 258 (90,8%) receberam a primeira dose, 157/258 (60,8%) e 68/258 (26,3%) receberam a segunda e terceira doses. Trabalhar em boates e consumir drogas ilícitas foram preditores de não completude do esquema vacinal (p<0,05). A elevada aceitabilidade da primeira dose da vacina, associada à baixa completude do esquema vacinal em profissionais do sexo, evidencia a necessidade de estratégia mais persuasiva que vá além da oferta da vacina no local de trabalho.

  10. Factors That Affect Oxygen Activation and Coupling of the Two Redox Cycles in the Aromatization Reaction Catalyzed by NikD, an Unusual Amino Acid Oxidase

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kommoju, Phaneeswara-Rao; Bruckner, Robert C.; Ferreira, Patricia

    2009-10-21

    NikD is a flavoprotein oxidase that catalyzes the oxidation of piperideine-2-carboxylate (P2C) to picolinate in a remarkable aromatization reaction comprising two redox cycles and at least one isomerization step. Tyr258 forms part of an 'aromatic cage' that surrounds the ring in picolinate and its precursors. Mutation of Tyr258 to Phe does not perturb the structure of nikD but does affect the coupling of the two redox cycles and causes a 10-fold decrease in turnover rate. Tyr258Phe catalyzes a quantitative two-electron oxidation of P2C, but only 60% of the resulting dihydropicolinate intermediate undergoes a second redox cycle to produce picolinate. Themore » mutation does not affect product yield with an alternate substrate (3,4-dehydro-l-proline) that is aromatized in a single two-electron oxidation step. Wild-type and mutant enzymes exhibit identical rate constants for oxidation of P2C to dihydropicolinate and isomerization of a reduced enzyme-dihydropicolinate complex. The observed rates are 200- and 10-fold faster, respectively, than the mutant turnover rate. Release of picolinate from Tyr258Phe is 100-fold faster than turnover. The presence of a bound substrate or product is a key factor in oxygen activation by wild-type nikD, as judged by the 10-75-fold faster rates observed for complexes of the reduced enzyme with picolinate, benzoate, or 1-cyclohexenoate, a 1-deaza-P2C analogue. The reduced Tyr258Phe-1-cyclohexenoate complex is 25-fold less reactive with oxygen than the wild-type complex. We postulate that mutation of Tyr258 causes subtle changes in active site dynamics that promote release of the reactive dihydropicolinate intermediate and disrupt the efficient synchronization of oxygen activation observed with wild-type nikD.« less

  11. p38α phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles.

    PubMed

    Ettelaie, Camille; Elkeeb, Azza M; Maraveyas, Anthony; Collier, Mary Elizabeth W

    2013-03-01

    We previously showed that the phosphorylation of Ser253 within the cytoplasmic domain of human tissue factor (TF) initiates the incorporation and release of this protein into cell-derived microparticles. Furthermore, subsequent phosphorylation of Ser258 terminates this process. However, the identity of the kinase responsible for the phosphorylation of Ser258 and mode of action of this enzyme remain unknown. In this study, p38α was identified as the proline-directed kinase capable of phosphorylating Ser258 specifically, and without any detectable activity towards Ser253. Furthermore, using synthetic peptides, it was shown that the Km for the reaction decreased by approximately 10 fold on substitution of Ser253 with phospho-Ser253. Either inhibition of p38 using SB202190 or knockdown of p38α expression in coronary artery endothelial cells overexpressing wild-type TF, resulted in decreased phosphorylation of Ser258, following activation of cells with PAR2-agonist peptide (PAR2-AP). In agreement with our previous data, inhibition of phosphorylation of this residue maintained the release of TF. Activation of PAR2 in cells transfected to overexpress TF, resulted in two separate peaks of p38 activity at approximately 40 and 120 min post-activation. Furthermore, overexpression of Ala253-substituted TF enhanced the second p38 activation peak. However, the second peak was absent in cells devoid of TF or in cells overexpressing the Asp253-substituted TF. Our data clearly identifies p38α as a kinase capable of phosphorylating Ser258 within the cytoplasmic domain of TF. Moreover, it appears that the presence of TF within the cells regulates the late activation of p38 and consequently the termination of TF release into microparticles. Copyright © 2012 Elsevier B.V. All rights reserved.

  12. [Path analysis of lifestyle habits to the metabolic syndrome].

    PubMed

    Zhu, Zhen-xin; Zhang, Cheng-qi; Tang, Fang; Song, Xin-hong; Xue, Fu-zhong

    2013-04-01

    To evaluate the relationship between lifestyle habits and the components of metabolic syndrome (MS). Based on the routine health check-up system in a certain Center for Health Management of Shandong Province, a longitudinal surveillance health check-up cohort from 2005 to 2010 was set up. There were 13 225 urban workers in Jinan included in the analysis. The content of the survey included demographic information, medical history, lifestyle habits, body mass index (BMI) and the level of blood pressure, fasting blood-glucose, and blood lipid, etc. The distribution of BMI, blood pressure, fasting blood-glucose, blood lipid and lifestyle habits between MS patients and non-MS population was compared, latent variables were extracted by exploratory factor analysis to determine the structure model, and then a partial least squares path model was constructed between lifestyle habits and the components of MS. Participants'age was (46.62 ± 12.16) years old. The overall prevalence of the MS was 22.43% (2967/13 225), 26.49% (2535/9570) in males and 11.82% (432/3655) in females. The prevalence of the MS was statistically different between males and females (χ(2) = 327.08, P < 0.01). Between MS patients and non-MS population, the difference of dietary habits was statistically significant (χ(2) = 166.31, P < 0.01) in MS patients, the rate of vegetarian, mixed and animal food was 23.39% (694/2967), 42.50% (1261/2967) and 34.11% (1012/2967) respectively, while in non-MS population was 30.80% (3159/10 258), 46.37% (4757/10 258), 22.83% (2342/10 258) respectively. Their alcohol consumption has statistical difference (χ(2) = 374.22, P < 0.01) in MS patients, the rate of never or past, occasional and regular drinking was 27.37% (812/2967), 24.71% (733/2967), 47.93% (1422/2967) respectively, and in non-MS population was 39.60% (4062/10 258), 31.36% (3217/10 258), 29.04% (2979/10 258) respectively. The difference of their smoking status was statistically significant (χ(2) = 115.86, P < 0.01) in MS patients, the rate of never or past, occasional and regular smoking was 59.72% (1772/2967), 6.24% (185/2967), 34.04% (1010/2967) respectively, while in non-MS population was 70.03% (7184/10 258), 5.35% (549/10 258), 24.61% (2525/10 258) respectively. Both lifestyle habits and the components of MS were attributable to only one latent variable. After adjustment for age and gender, the path coefficient between the latent component of lifestyle habits and the latent component of MS was 0.22 with statistical significance (t = 6.46, P < 0.01) through bootstrap test. Reliability and validity of the model:the lifestyle latent variable: average variance extracted was 0.53, composite reliability was 0.77 and Cronbach's a was 0.57. The MS latent variable: average variance extracted was 0.45, composite reliability was 0.76 and Cronbach's a was 0.59. Unhealthy lifestyle habits are closely related to MS. Meat diet, excessive drinking and smoking are risk factors for MS.

  13. DIVWAG Model Documentation. Volume II. Programmer/Analyst Manual. Part 1.

    DTIC Science & Technology

    1976-07-01

    that portion of the Intelligence and Control Model regulating supporting aerial fire and artillery. flight corridor: a rectangular area centered along...0.0. N N,( ON ~~ *0,U0 0 ZMN .. .6 N 6’ N N~. C4NANCy 0 -0 0 4.0 4 - 񓿀.104M 2 0 .i..i.6 C4 - - 0) 14 U.U 00 cocoa eeoc a 0iW z0 7 A 0) ;C a a . z 0...modifiers and all required data elements. (1) There are two basic rules regulating the entry of data elements when required by an order or an order modifier

  14. First finding of a mammoth female ( Mammuthus primigenius Blum.) on the Taimyr Peninsula

    NASA Astrophysics Data System (ADS)

    Kirillova, I. V.; Shidlovskiy, F. K.; Khasanov, B. F.

    2011-02-01

    The first finding of a mammoth female skeleton from the Kastykhtakh River valley, Taimyr Peninsula, is described. The skeleton consists of 104 elements including the skull and lower jaw; small distal limb bones, tail vertebrae, and one tusk are absent. There are teeth of the two last changes in the jaws. The skeleton elements have individual features: sigmoid contact of the low second and third molars, crack in the neural arch of the atlas, and false joint and calluses on places of the rib fractures. The calibrated radiocarbon dating of the tubular bone is 32 070-30 565 yr (Kargin interglacial time).

  15. 77 FR 69781 - Enhanced Natural Gas Market Transparency

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-21

    ... be filed in native applications or print-to-PDF format and not in a scanned format, at http://www.... FERC, 661 F.3d 258 (5th Cir. 2011). \\17\\ Order Granting Motion to Clarify Opinion, Texas Pipelines Ass'n v. FERC, 661 F.3d 258 (Dec. 20. 2011). 8. In addition to adding the natural gas market...

  16. 40 CFR 466.13 - New source performance standards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Oil and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units....87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within...

  17. 40 CFR 466.13 - New source performance standards.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Oil and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units....87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within...

  18. 40 CFR 466.13 - New source performance standards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Oil and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units....87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within...

  19. 77 FR 58773 - Drawbridge Operation Regulations; James River, Newport News, VA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... Operation Regulations; James River, Newport News, VA AGENCY: Coast Guard, DHS. ACTION: Notice of temporary... schedule that governs the US 17/258 Bridge across the James River, mile 5.0, at Newport News, VA. The... 17/258 Bridge over the James River, mile 5.0, at Newport News, VA opens on signal as required by 33...

  20. 37 CFR 258.3 - Royalty fee for secondary transmission of analog signals of broadcast stations by satellite...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Royalty fee for secondary... AND PROCEDURES ADJUSTMENT OF ROYALTY FEE FOR SECONDARY TRANSMISSIONS BY SATELLITE CARRIERS § 258.3 Royalty fee for secondary transmission of analog signals of broadcast stations by satellite carriers. (a...

  1. 77 FR 61428 - Notice of Meeting for Star-Spangled Banner National Historic Trail Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-09

    ... preregister via email at [email protected] or telephone: (757) 258-8914; a written summary of comments... public comment session should register via email at [email protected] or telephone: (757) 258-8914. For those wishing to make comments, please provide a written summary of your comments prior to the...

  2. 77 FR 64352 - Notice of Meeting for Captain John Smith Chesapeake National Historic Trail Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-19

    ... should preregister via email at [email protected] or telephone: (757) 258-8914; a written summary... meeting and/or participate in the public comment session should register via email at [email protected] or telephone: (757) 258-8914. For those wishing to make comments, please provide a written summary...

  3. 78 FR 59368 - Notice of Joint Meeting for Captain John Smith Chesapeake National Historic Trail Advisory...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-26

    ... comment for the public comment session should register via email to [email protected] or telephone (757) 258-8914. For those wishing to make comments, please provide a written summary of your comments...) 258-8914 or email [email protected] . SUPPLEMENTARY INFORMATION: Under section 10(a)(2) of the...

  4. Three ulcerative colitis susceptibility loci are associated with primary sclerosing cholangitis and indicate a role for IL2, REL, and CARD9.

    PubMed

    Janse, Marcel; Lamberts, Laetitia E; Franke, Lude; Raychaudhuri, Soumya; Ellinghaus, Eva; Muri Boberg, Kirsten; Melum, Espen; Folseraas, Trine; Schrumpf, Erik; Bergquist, Annika; Björnsson, Einar; Fu, Jingyuan; Jan Westra, Harm; Groen, Harry J M; Fehrmann, Rudolf S N; Smolonska, Joanna; van den Berg, Leonard H; Ophoff, Roel A; Porte, Robert J; Weismüller, Tobias J; Wedemeyer, Jochen; Schramm, Christoph; Sterneck, Martina; Günther, Rainer; Braun, Felix; Vermeire, Severine; Henckaerts, Liesbet; Wijmenga, Cisca; Ponsioen, Cyriel Y; Schreiber, Stefan; Karlsen, Tom H; Franke, Andre; Weersma, Rinse K

    2011-06-01

    Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease characterized by inflammation and fibrosis of the bile ducts. Both environmental and genetic factors contribute to its pathogenesis. To further clarify its genetic background, we investigated susceptibility loci recently identified for ulcerative colitis (UC) in a large cohort of 1,186 PSC patients and 1,748 controls. Single nucleotide polymorphisms (SNPs) tagging 13 UC susceptibility loci were initially genotyped in 854 PSC patients and 1,491 controls from Benelux (331 cases, 735 controls), Germany (265 cases, 368 controls), and Scandinavia (258 cases, 388 controls). Subsequently, a joint analysis was performed with an independent second Scandinavian cohort (332 cases, 257 controls). SNPs at chromosomes 2p16 (P-value 4.12 × 10(-4) ), 4q27 (P-value 4.10 × 10(-5) ), and 9q34 (P-value 8.41 × 10(-4) ) were associated with PSC in the joint analysis after correcting for multiple testing. In PSC patients without inflammatory bowel disease (IBD), SNPs at 4q27 and 9q34 were nominally associated (P < 0.05). We applied additional in silico analyses to identify likely candidate genes at PSC susceptibility loci. To identify nonrandom, evidence-based links we used GRAIL (Gene Relationships Across Implicated Loci) analysis showing interconnectivity between genes in six out of in total nine PSC-associated regions. Expression quantitative trait analysis from 1,469 Dutch and UK individuals demonstrated that five out of nine SNPs had an effect on cis-gene expression. These analyses prioritized IL2, CARD9, and REL as novel candidates. We have identified three UC susceptibility loci to be associated with PSC, harboring the putative candidate genes REL, IL2, and CARD9. These results add to the scarce knowledge on the genetic background of PSC and imply an important role for both innate and adaptive immunological factors. Copyright © 2011 American Association for the Study of Liver Diseases.

  5. 76 FR 17861 - Notice of Public Information Collection(s) Being Reviewed by the Federal Communications...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-31

    ... required by the Paperwork Reduction Act (PRA) of 1995, 44 U.S.C. 3501-3520. Comments are requested... accuracy of the Commission's burden estimate; (c) ways to enhance the quality, utility, and clarity of the... is found at Section 258 [47 U.S.C. 258] Illegal Changes in Subscriber Carrier Selections, Public Law...

  6. The Pax gene family: Highlights from cephalopods

    PubMed Central

    Baratte, Sébastien; Andouche, Aude; Bonnaud-Ponticelli, Laure

    2017-01-01

    Pax genes play important roles in Metazoan development. Their evolution has been extensively studied but Lophotrochozoa are usually omitted. We addressed the question of Pax paralog diversity in Lophotrochozoa by a thorough review of available databases. The existence of six Pax families (Pax1/9, Pax2/5/8, Pax3/7, Pax4/6, Paxβ, PoxNeuro) was confirmed and the lophotrochozoan Paxβ subfamily was further characterized. Contrary to the pattern reported in chordates, the Pax2/5/8 family is devoid of homeodomain in Lophotrochozoa. Expression patterns of the three main pax classes (pax2/5/8, pax3/7, pax4/6) during Sepia officinalis development showed that Pax roles taken as ancestral and common in metazoans are modified in S. officinalis, most likely due to either the morphological specificities of cephalopods or to their direct development. Some expected expression patterns were missing (e.g. pax6 in the developing retina), and some expressions in unexpected tissues have been found (e.g. pax2/5/8 in dermal tissue and in gills). This study underlines the diversity and functional plasticity of Pax genes and illustrates the difficulty of using probable gene homology as strict indicator of homology between biological structures. PMID:28253300

  7. A modularized pulse programmer for NMR spectroscopy

    NASA Astrophysics Data System (ADS)

    Mao, Wenping; Bao, Qingjia; Yang, Liang; Chen, Yiqun; Liu, Chaoyang; Qiu, Jianqing; Ye, Chaohui

    2011-02-01

    A modularized pulse programmer for a NMR spectrometer is described. It consists of a networked PCI-104 single-board computer and a field programmable gate array (FPGA). The PCI-104 is dedicated to translate the pulse sequence elements from the host computer into 48-bit binary words and download these words to the FPGA, while the FPGA functions as a sequencer to execute these binary words. High-resolution NMR spectra obtained on a home-built spectrometer with four pulse programmers working concurrently demonstrate the effectiveness of the pulse programmer. Advantages of the module include (1) once designed it can be duplicated and used to construct a scalable NMR/MRI system with multiple transmitter and receiver channels, (2) it is a totally programmable system in which all specific applications are determined by software, and (3) it provides enough reserve for possible new pulse sequences.

  8. Chemistry of the superheavy elements.

    PubMed

    Schädel, Matthias

    2015-03-13

    The quest for superheavy elements (SHEs) is driven by the desire to find and explore one of the extreme limits of existence of matter. These elements exist solely due to their nuclear shell stabilization. All 15 presently 'known' SHEs (11 are officially 'discovered' and named) up to element 118 are short-lived and are man-made atom-at-a-time in heavy ion induced nuclear reactions. They are identical to the transactinide elements located in the seventh period of the periodic table beginning with rutherfordium (element 104), dubnium (element 105) and seaborgium (element 106) in groups 4, 5 and 6, respectively. Their chemical properties are often surprising and unexpected from simple extrapolations. After hassium (element 108), chemistry has now reached copernicium (element 112) and flerovium (element 114). For the later ones, the focus is on questions of their metallic or possibly noble gas-like character originating from interplay of most pronounced relativistic effects and electron-shell effects. SHEs provide unique opportunities to get insights into the influence of strong relativistic effects on the atomic electrons and to probe 'relativistically' influenced chemical properties and the architecture of the periodic table at its farthest reach. In addition, they establish a test bench to challenge the validity and predictive power of modern fully relativistic quantum chemical models. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

  9. Records of anthropogenic antimony in the glacial snow from the southeastern Tibetan Plateau

    NASA Astrophysics Data System (ADS)

    Zhang, Yulan; Kang, Shichang; Chen, Pengfei; Li, Xiaofei; Liu, Yajun; Gao, Tanguang; Guo, Junming; Sillanpää, Mika

    2016-12-01

    Antimony (Sb) is a ubiquitous element in the environment that is potentially toxic at very low concentrations. In this study, surface snow/ice and snowpit samples were collected from four glaciers in the southeastern Tibetan Plateau in June 2015. The concentrations of Sb and other elements were measured in these samples. The results showed that the average concentration of Sb was approximately 2.58 pg g-1 with a range of 1.64-9.20 pg g-1. The average Sb concentration in the study area was comparable to that recorded in a Mt. Everest ice core and higher than that in Arctic and Antarctic snow/ice but much lower than that in Tien Shan and Alps ice cores. Sb presented different variations with other toxic elements (Pb and Cr) and a crustal element (Al) in the three snowpits, which indicated the impact of a different source or post-deposition processes. The enrichment factor of Sb was larger than 10, suggesting that anthropogenic sources provided important contributions to Sb deposition in the glaciers. The Sb in the glacial snow was mainly loaded in the fourth component in principal component analysis, exhibiting discrepancies with crustal elements (Fe and Ca) and other toxic metals (Pb). Backward trajectories revealed that the air mass arriving at the southeastern Tibetan Plateau mostly originated from the Bay of Bengal and the South Asia in June. Thus, pollutants from the South Asia could play an important role in Sb deposition in the studied region. The released Sb from glacier meltwater in the Tibetan Plateau and surrounding areas might pose a risk to the livelihoods and well-being of those in downstream regions.

  10. Subject-specific finite element modelling of the human foot complex during walking: sensitivity analysis of material properties, boundary and loading conditions.

    PubMed

    Akrami, Mohammad; Qian, Zhihui; Zou, Zhemin; Howard, David; Nester, Chris J; Ren, Lei

    2018-04-01

    The objective of this study was to develop and validate a subject-specific framework for modelling the human foot. This was achieved by integrating medical image-based finite element modelling, individualised multi-body musculoskeletal modelling and 3D gait measurements. A 3D ankle-foot finite element model comprising all major foot structures was constructed based on MRI of one individual. A multi-body musculoskeletal model and 3D gait measurements for the same subject were used to define loading and boundary conditions. Sensitivity analyses were used to investigate the effects of key modelling parameters on model predictions. Prediction errors of average and peak plantar pressures were below 10% in all ten plantar regions at five key gait events with only one exception (lateral heel, in early stance, error of 14.44%). The sensitivity analyses results suggest that predictions of peak plantar pressures are moderately sensitive to material properties, ground reaction forces and muscle forces, and significantly sensitive to foot orientation. The maximum region-specific percentage change ratios (peak stress percentage change over parameter percentage change) were 1.935-2.258 for ground reaction forces, 1.528-2.727 for plantar flexor muscles and 4.84-11.37 for foot orientations. This strongly suggests that loading and boundary conditions need to be very carefully defined based on personalised measurement data.

  11. Measurements of B --> {pi,eta,eta;{'}}lnu_{l} branching fractions and determination of |V_{ub}| with semileptonically tagged B mesons.

    PubMed

    Aubert, B; Bona, M; Karyotakis, Y; Lees, J P; Poireau, V; Prencipe, E; Prudent, X; Tisserand, V; Garra Tico, J; Grauges, E; Lopez, L; Palano, A; Pappagallo, M; Eigen, G; Stugu, B; Sun, L; Abrams, G S; Battaglia, M; Brown, D N; Cahn, R N; Jacobsen, R G; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Lynch, G; Osipenkov, I L; Ronan, M T; Tackmann, K; Tanabe, T; Hawkes, C M; Soni, N; Watson, A T; Koch, H; Schroeder, T; Walker, D; Asgeirsson, D J; Cuhadar-Donszelmann, T; Fulsom, B G; Hearty, C; Mattison, T S; McKenna, J A; Barrett, M; Khan, A; Teodorescu, L; Blinov, V E; Bukin, A D; Buzykaev, A R; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M; Martin, E C; Stoker, D P; Abachi, S; Buchanan, C; Gary, J W; Liu, F; Long, O; Shen, B C; Vitug, G M; Yasin, Z; Zhang, L; Sharma, V; Campagnari, C; Hong, T M; Kovalskyi, D; Mazur, M A; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Schalk, T; Schumm, B A; Seiden, A; Wang, L; Wilson, M G; Winstrom, L O; Cheng, C H; Doll, D A; Echenard, B; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Andreassen, R; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Blanc, F; Bloom, P C; Ford, W T; Gaz, A; Hirschauer, J F; Kreisel, A; Nagel, M; Nauenberg, U; Smith, J G; Ulmer, K A; Wagner, S R; Ayad, R; Soffer, A; Toki, W H; Wilson, R J; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Karbach, M; Merkel, J; Petzold, A; Spaan, B; Wacker, K; Kobel, M J; Mader, W F; Nogowski, R; Schubert, K R; Schwierz, R; Sundermann, J E; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Thiebaux, Ch; Verderi, M; Clark, P J; Gradl, W; Playfer, S; Watson, J E; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cecchi, A; Cibinetto, G; Franchini, P; Luppi, E; Negrini, M; Petrella, A; Piemontese, L; Santoro, V; Baldini-Ferroli, R; Calcaterra, A; de Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Contri, R; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Chaisanguanthum, K S; Morii, M; Dubitzky, R S; Marks, J; Schenk, S; Uwer, U; Klose, V; Lacker, H M; De Nardo, G; Lista, L; Monorchio, D; Onorato, G; Sciacca, C; Bard, D J; Dauncey, P D; Nash, J A; Panduro Vazquez, W; Tibbetts, M; Behera, P K; Chai, X; Charles, M J; Mallik, U; Cochran, J; Crawley, H B; Dong, L; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Gao, Y Y; Gritsan, A V; Guo, Z J; Lae, C K; Denig, A G; Fritsch, M; Schott, G; Arnaud, N; Béquilleux, J; D'Orazio, A; Davier, M; Firmino da Costa, J; Grosdidier, G; Höcker, A; Lepeltier, V; Le Diberder, F; Lutz, A M; Pruvot, S; Roudeau, P; Schune, M H; Serrano, J; Sordini, V; Stocchi, A; Wormser, G; Lange, D J; Wright, D M; Bingham, I; Burke, J P; Chavez, C A; Fry, J R; Gabathuler, E; Gamet, R; Hutchcroft, D E; Payne, D J; Touramanis, C; Bevan, A J; George, K A; Di Lodovico, F; Sacco, R; Sigamani, M; Cowan, G; Flaecher, H U; Hopkins, D A; Paramesvaran, S; Salvatore, F; Wren, A C; Brown, D N; Davis, C L; Alwyn, K E; Barlow, N R; Barlow, R J; Chia, Y M; Edgar, C L; Lafferty, G D; West, T J; Yi, J I; Anderson, J; Chen, C; Jawahery, A; Roberts, D A; Simi, G; Tuggle, J M; Dallapiccola, C; Hertzbach, S S; Li, X; Salvati, E; Saremi, S; Cowan, R; Dujmic, D; Fisher, P H; Koeneke, K; Sciolla, G; Spitznagel, M; Taylor, F; Yamamoto, R K; Zhao, M; McLachlin, S E; Patel, P M; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Cremaldi, L; Eschenburg, V; Godang, R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Simard, M; Taras, P; Viaud, F B; Nicholson, H; Baak, M A; Raven, G; Snoek, H L; Jessop, C P; Knoepfel, K J; Losecco, J M; Wang, W F; Benelli, G; Corwin, L A; Honscheid, K; Kagan, H; Kass, R; Morris, J P; Rahimi, A M; Regensburger, J J; Sekula, S J; Wong, Q K; Blount, N L; Brau, J; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Castelli, G; Gagliardi, N; Margoni, M; Morandin, M; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Del Amo Sanchez, P; Ben-Haim, E; Briand, H; Calderini, G; Chauveau, J; David, P; Del Buono, L; Hamon, O; Leruste, Ph; Ocariz, J; Perez, A; Prendki, J; Gladney, L; Biasini, M; Covarelli, R; Manoni, E; Angelini, C; Batignani, G; Bettarini, S; Carpinelli, M; Cervelli, A; Forti, F; Giorgi, M A; Lusiani, A; Marchiori, G; Morganti, M; Neri, N; Paoloni, E; Rizzo, G; Walsh, J J; Biesiada, J; Lopes Pegna, D; Lu, C; Olsen, J; Smith, A J S; Telnov, A V; Anulli, F; Baracchini, E; Cavoto, G; Del Re, D; Di Marco, E; Faccini, R; Ferrarotto, F; Ferroni, F; Gaspero, M; Jackson, P D; Li Gioi, L; Mazzoni, M A; Morganti, S; Piredda, G; Polci, F; Renga, F; Voena, C; Ebert, M; Hartmann, T; Schröder, H; Waldi, R; Adye, T; Franek, B; Olaiya, E O; Roethel, W; Wilson, F F; Emery, S; Escalier, M; Esteve, L; Gaidot, A; Ganzhur, S F; Hamel de Monchenault, G; Kozanecki, W; Vasseur, G; Yèche, Ch; Zito, M; Chen, X R; Liu, H; Park, W; Purohit, M V; White, R M; Wilson, J R; Allen, M T; Aston, D; Bartoldus, R; Bechtle, P; Benitez, J F; Cenci, R; Coleman, J P; Convery, M R; Dingfelder, J C; Dorfan, J; Dubois-Felsmann, G P; Dunwoodie, W; Field, R C; Gabareen, A M; Gowdy, S J; Graham, M T; Grenier, P; Hast, C; Innes, W R; Kaminski, J; Kelsey, M H; Kim, H; Kim, P; Kocian, M L; Leith, D W G S; Li, S; Lindquist, B; Luitz, S; Luth, V; Lynch, H L; Macfarlane, D B; Marsiske, H; Messner, R; Muller, D R; Neal, H; Nelson, S; O'Grady, C P; Ofte, I; Perazzo, A; Perl, M; Ratcliff, B N; Roodman, A; Salnikov, A A; Schindler, R H; Schwiening, J; Snyder, A; Su, D; Sullivan, M K; Suzuki, K; Swain, S K; Thompson, J M; Va'vra, J; Wagner, A P; Weaver, M; West, C A; Wisniewski, W J; Wittgen, M; Wright, D H; Wulsin, H W; Yarritu, A K; Yi, K; Young, C C; Ziegler, V; Burchat, P R; Edwards, A J; Majewski, S A; Miyashita, T S; Petersen, B A; Wilden, L; Ahmed, S; Alam, M S; Bula, R; Ernst, J A; Pan, B; Saeed, M A; Zain, S B; Spanier, S M; Wogsland, B J; Eckmann, R; Ritchie, J L; Ruland, A M; Schilling, C J; Schwitters, R F; Drummond, B W; Izen, J M; Lou, X C; Bianchi, F; Gamba, D; Pelliccioni, M; Bomben, M; Bosisio, L; Cartaro, C; Della Ricca, G; Lanceri, L; Vitale, L; Azzolini, V; Lopez-March, N; Martinez-Vidal, F; Milanes, D A; Oyanguren, A; Albert, J; Banerjee, Sw; Bhuyan, B; Choi, H H F; Hamano, K; Kowalewski, R; Lewczuk, M J; Nugent, I M; Roney, J M; Sobie, R J; Gershon, T J; Harrison, P F; Ilic, J; Latham, T E; Mohanty, G B; Band, H R; Chen, X; Dasu, S; Flood, K T; Pan, Y; Pierini, M; Prepost, R; Vuosalo, C O; Wu, S L

    2008-08-22

    We report measurements of branching fractions for the decays B-->Plnu_{l}, where P are the pseudoscalar charmless mesons pi;{-}, pi;{0}, eta and eta;{'}, based on 348 fb;{-1} of data collected with the BABAR detector, using B0 and B+ mesons found in the recoil of a second B meson decaying as B-->D;{(*)}lnu_{l}. Assuming isospin symmetry, we combine pionic branching fractions to obtain B(B;{0}-->pi;{-}l;{+}nu_{l})=(1.54+/-0.17_{(stat)}+/-0.09_{(syst)})x10;{-4}; we find 3.2sigma evidence of the decay B;{+}-->etal;{+}nu_{l} and measure its branching fraction to be (0.64+/-0.20_{(stat)}+/-0.03_{(syst)})x10;{-4}, and determine B(B;{+}-->eta;{'}l;{+}nu_{l})<0.47x10;{-4} to 90% confidence level. Using partial branching fractions for the pionic decays in ranges of the momentum transfer and a variety of form factor calculation, we obtain values of the magnitude of the Cabibbo-Kobayashi-Maskawa matrix element |V_{ub}| in ranging from 3.6x10;{-3} to 4.1x10;{-3}.

  12. Propulsion Systems

    DTIC Science & Technology

    2011-03-31

    Manual 91 - 710 Vol. 2 ( AFSPCMAN 91 - 710 V2). 8. Astrium EADS. 2010. cs.astrium.eads.net. April. 9. Astrium EADS. 2010. : http...of the High Energy Density Matter (HEDM) Conference. Albuquerque, NM, February 24-27, (Phillips Lab PL-CP- 91 -3003, p. 3 ). 104. Sullivan, D. J...Chapter 3 . DATES COVERED (From - To) 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER Chapter 18 - Propulsion Systems 5b. GRANT NUMBER 5c. PROGRAM ELEMENT

  13. Examining alcohol consumption with the theory of planned behaviour: Do health and alcohol knowledge play a role?

    PubMed

    Hasking, Penelope; Schofield, Lachlan

    2015-01-01

    We used the theory of planned behaviour (TPB) to investigate factors associated with alcohol consumption among university students, and to examine whether general or alcohol-specific health knowledge acts as a moderator in the relationship between elements of the theory and drinking behaviour. Participants were 258 Australian undergraduate university students (79% female) who completed an online questionnaire, assessing the constructs of interest. The hypothesis that intentions and behaviour would be successfully predicted using the theory was generally supported. Little evidence for the moderating effect of knowledge on the TPB variables was observed, although both general and alcohol-specific health knowledge moderated the relationship between intentions and behaviours. Contrary to expectation, more accurate knowledge strengthened this relationship. Further work is necessary to investigate the role of knowledge in limiting alcohol-related harms.

  14. Silica dust, diesel exhaust, and painting work are the significant occupational risk factors for lung cancer in nonsmoking Chinese men

    PubMed Central

    Tse, L A; Yu, IT-s; Au, J S K; Qiu, H; Wang, X-r

    2011-01-01

    Background: Few epidemiological studies have explored the associations between occupational exposures and lung cancer in lifelong nonsmoking men. Methods: We obtained lifetime occupational history and other relevant information for 132 newly diagnosed lung cancer cases among nonsmoking Chinese men and 536 nonsmoking community referents. Unconditional multiple logistic regression analysis was performed to estimate the odds ratio (OR) of lung cancer for specific occupational exposures. Results: Significantly increased lung cancer risk was found for nonsmoking workers occupationally exposed to silica dust (OR=2.58, 95% confidence interval (CI): 1.11, 6.01), diesel exhaust (OR=3.47, 95% CI: 1.08, 11.14), spray painting (OR=2.81, 95% CI: 1.14, 6.93), and nonspray painting work (OR=2.36, 95% CI: 1.04, 5.37). Silica dust exposure was associated with a significantly increased risk of adenocarcinoma (OR=2.91, 95% CI: 1.10, 7.68). We observed a positive gradient of all lung cancers and of adenocarcinoma with duration of employment for workers exposed to silica dust and spray painting. Conclusion: This study found an increased risk of lung cancer among nonsmoking Chinese men occupationally exposed to silica dust, diesel exhaust, and painting work. PMID:21102581

  15. Reduced graphene oxide modified smart conducting paper for cancer biosensor.

    PubMed

    Kumar, Saurabh; Kumar, Suveen; Srivastava, Saurabh; Yadav, Birendra K; Lee, Seung H; Sharma, Jai G; Doval, Dinesh C; Malhotra, Bansi D

    2015-11-15

    We report results of the studies relating to the fabrication of a paper based sensor comprising of poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) ( PSS) and reduced graphene oxide (RGO) composite. The effect of various solvents like methanol, ethylene glycol and H2SO4 on the electrical conductivity of PSS coated Whatman paper has been investigated. The conductivity of this solution processed conducting paper significantly increases from ~1.16×10(-4) S cm(-1) up to ~3.57×10(-2) S cm(-1) (~300 times) on treatment with ethylene glycol. The observed significant increase in electrical conductivity is due to conformational rearrangement in the polymer and is due to strong non-covalent cooperative interaction between PEDOT and the cellulose molecules. Further, incorporation of RGO into the conducting paper results in improved electrochemical performance and signal stability. This paper electrode is a promising alternative over the expensive conventional electrodes (ITO, gold and glassy carbon), that are known to have limited application in smart point-of-care (POC) devices. This low cost, flexible and environment friendly conducting paper based biosensor utilized for cancer biomarker (carcinoembryonic antigen, CEA) detection reveals high sensitivity of 25.8 µA ng(-1) mL cm(-2) in the physiological range, 1-10 ng mL(-1). Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Relationship between sickness presenteeism and awareness and presence or absence of systems for return to work among workers with mental health problems in Japan: an Internet-based cross-sectional study.

    PubMed

    Doki, Shotaro; Sasahara, Shinichiro; Suzuki, Shun; Hirai, Yasuhito; Oi, Yuichi; Usami, Kazuya; Matsuzaki, Ichiyo

    2015-01-01

    The objective of this study was to clarify the relationship between sickness presenteeism and the degree of awareness of company regulations regarding financial compensation for absenteeism. An Internet-based survey of 258 full-time workers who had more than 28 days of sickness absence due to mental health problems was conducted. Workers were categorized as having either low or high sickness presenteeism, and awareness and presence or absence of systems for return to work and duration of financial compensation were compared between groups. The following factors were significantly related to high sickness presenteeism based on logistic regression analyses: working for a private company (odds ratio [OR]=2.57; 95% confidence interval [CI]=1.10-5.99); recognition of a gradual resumption system (OR=3.89, 95% CI=1.02-14.81); and awareness regarding the duration of financial compensation (OR=1.04, 95% CI=1.01-1.07). No relationship was found between mental sickness presenteeism and presence or absence of systems for return to work in our multivariate analysis; however, a relationship was apparent between sickness presenteeism and characteristics of the workers' companies. These results are expected to contribute to research involving human resources and occupational health.

  17. PCR-based detection of Toxoplasma gondii DNA in blood and ocular samples for diagnosis of ocular toxoplasmosis.

    PubMed

    Bourdin, C; Busse, A; Kouamou, E; Touafek, F; Bodaghi, B; Le Hoang, P; Mazier, D; Paris, L; Fekkar, A

    2014-11-01

    PCR detection of Toxoplasma gondii in blood has been suggested as a possibly efficient method for the diagnosis of ocular toxoplasmosis (OT) and furthermore for genotyping the strain involved in the disease. To assess this hypothesis, we performed PCR with 121 peripheral blood samples from 104 patients showing clinical and/or biological evidence of ocular toxoplasmosis and from 284 (258 patients) controls. We tested 2 different extraction protocols, using either 200 μl (small volume) or 2 ml (large volume) of whole blood. Sensitivity was poor, i.e., 4.1% and 25% for the small- and large-volume extractions, respectively. In comparison, PCR with ocular samples yielded 35.9% sensitivity, while immunoblotting and calculation of the Goldmann-Witmer coefficient yielded 47.6% and 72.3% sensitivities, respectively. Performing these three methods together provided 89.4% sensitivity. Whatever the origin of the sample (ocular or blood), PCR provided higher sensitivity for immunocompromised patients than for their immunocompetent counterparts. Consequently, PCR detection of Toxoplasma gondii in blood samples cannot currently be considered a sufficient tool for the diagnosis of OT, and ocular sampling remains necessary for the biological diagnosis of OT. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  18. Structural Impact of Three Parkinsonism-Associated Missense Mutations on Human DJ-1

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lakshminarasimhan, M.; Maldonado, M.T.; Zhou, W.

    2009-05-20

    A number of missense mutations in the oxidative stress response protein DJ-1 are implicated in rare forms of familial Parkinsonism. The best-characterized Parkinsonian DJ-1 missense mutation, L166P, disrupts homodimerization and results in a poorly folded protein. The molecular basis by which the other Parkinsonism-associated mutations disrupt the function of DJ-1, however, is incompletely understood. In this study we show that three different Parkinsonism-associated DJ-1 missense mutations (A104T, E163K, and M26I) reduce the thermal stability of DJ-1 in solution by subtly perturbing the structure of DJ-1 without causing major folding defects or loss of dimerization. Atomic resolution X-ray crystallography shows thatmore » the A104T substitution introduces water and a discretely disordered residue into the core of the protein, E163K disrupts a key salt bridge with R145, and M26I causes packing defects in the core of the dimer. The deleterious effect of each Parkinsonism-associated mutation on DJ-1 is dissected by analysis of engineered substitutions (M26L, A104V, and E163K/R145E) that partially alleviate each of the defects introduced by the A104T, E163K and M26I mutations. In total, our results suggest that the protective function of DJ-1 can be compromised by diverse perturbations in its structural integrity, particularly near the junctions of secondary structural elements.« less

  19. 40 CFR 264.258 - Closure and post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Waste Piles § 264.258 Closure and post-closure care. (a) At closure, the owner or operator must remove... that apply to landfills (§ 264.310). (c)(1) The owner or operator of a waste pile that does not comply...(c) or § 264.251(b), must: (i) Include in the closure plan for the pile under § 264.112 both a plan...

  20. 40 CFR 466.43 - New source performance standards.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....55 10.60 0.79 Oil and grease 168.23 12.60 168.23 12.60 TSS 252.35 18.91 201.88 15.12 pH (1) (1) (1....32 Iron 4.24 0.32 2.17 0.16 Oil and grease 34.46 2.58 34.46 2.58 TSS 51.69 3.87 41.35 3.10 pH (1) (1...

  1. 42 CFR 137.258 - Does the Self-Governance Tribe have a right to a hearing prior to a non-immediate reassumption...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Does the Self-Governance Tribe have a right to a... PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES INDIAN HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES TRIBAL SELF-GOVERNANCE Reassumption § 137.258 Does the Self-Governance Tribe...

  2. Examining Two Sets of Introgression Lines in Rice (Oryza sativa L.) Reveals Favorable Alleles that Improve Grain Zn and Fe Concentrations

    PubMed Central

    Hu, Xia; Cheng, Li-Rui; Xu, Jian-Long; Shi, Yu-Min; Li, Zhi-Kang

    2015-01-01

    In the modern world, the grain mineral concentration (GMC) in rice (Oryza sativa L.) not only includes important micronutrient elements such as iron (Fe) and zinc (Zn), but it also includes toxic heavy metal elements, especially cadmium (Cd) and lead (Pb). To date, the genetic mechanisms underlying the regulation of GMC, especially the genetic background and G × E effects of GMC, remain largely unknown. In this study, we adopted two sets of backcross introgression lines (BILs) derived from IR75862 (a Zn-dense rice variety) as the donor parent and two elite indica varieties, Ce258 and Zhongguangxiang1, as recurrent parents to detect QTL affecting GMC traits including Fe, Zn, Cd and Pb concentrations in two environments. We detected a total of 22 loci responsible for GMC traits, which are distributed on all 12 rice chromosomes except 5, 9 and 10. Six genetic overlap (GO) regions affecting multiple elements were found, in which most donor alleles had synergistic effects on GMC. Some toxic heavy metal-independent loci (such as qFe1, qFe2 and qZn12) and some regions that have opposite genetic effects on micronutrient (Fe and Zn) and heavy metal element (Pb) concentrations (such as GO-IV) may be useful for marker-assisted biofortification breeding in rice. We discuss three important points affecting biofortification breeding efforts in rice, including correlations between different GMC traits, the genetic background effect and the G × E effect. PMID:26161553

  3. Targeting fibroblast growth factor receptors blocks PI3K/AKT signaling, induces apoptosis, and impairs mammary tumor outgrowth and metastasis.

    PubMed

    Dey, Julien H; Bianchi, Fabrizio; Voshol, Johannes; Bonenfant, Debora; Oakeley, Edward J; Hynes, Nancy E

    2010-05-15

    Members of the fibroblast growth factor receptor (FGFR) family have essential roles in normal physiology and in cancer where they control diverse processes. FGFRs have been associated with breast cancer development. Thus, models to study the role of FGFR in breast cancer and their targeting potential are important. We present an in vitro and in vivo analysis of FGFRs in the breast cancer model cell lines 67NR and 4T1. We show that both tumor cell lines coexpress FGFRs and ligands and display autocrine FGFR signaling activity. Fibroblast growth factor receptor substrate 2 (FRS2), a downstream mediator of FGFR, is constitutively tyrosine phosphorylated and multiple signaling pathways are active. Treatment of 67NR and 4T1 cultures with TKI258, an FGFR tyrosine kinase inhibitor (TKI), caused a rapid decrease in FRS2 phosphorylation; decreased the activity of extracellular signal-regulated kinase 1/2 (ERK1/2), AKT, and phospholipase Cgamma; and blocked proliferation of both tumor lines. Furthermore, TKI258 induced 4T1 apoptotic cell death via blockade of the phosphoinositide 3-kinase/AKT pathway. In vivo, one dose of TKI258 rapidly lowered FRS2 phosphorylation and ERK1/2 and AKT activity in mammary tumors. Long-term TKI258 treatment of 4T1 tumor- and 67NR tumor-bearing mice had a significant effect on primary tumor outgrowth and 4T1 tumor-induced lung metastases. A microarray analysis was carried out to identify targets with roles in TKI258 antitumor activity and potential prognostic markers in human breast tumors. Of interest are the downregulated matrix metalloproteases (MMP), in particular MMP9, which is essential for metastatic spread of 4T1 tumors. (c)2010 AACR.

  4. RUBIDIUM ABUNDANCES IN THE GLOBULAR CLUSTERS NGC 6752, NGC 1904, AND NGC 104 (47 Tuc)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    D'Orazi, Valentina; Lugaro, Maria; Campbell, Simon W.

    2013-10-10

    Large star-to-star variations of the abundances of proton-capture elements, such as Na and O, in globular clusters (GCs) are interpreted as the effect of internal pollution resulting from the presence of multiple stellar populations. To better constrain this scenario, we investigate the abundance distribution of the heavy element rubidium (Rb) in NGC 6752, NGC 1904, and NGC 104 (47 Tuc). Combining the results from our sample with those in the literature, we found that Rb exhibits no star-to-star variations, regardless of cluster metallicity, with the possible intriguing, although very uncertain, exception of the metal-rich bulge cluster NGC 6388. If nomore » star-to-star variations can be confirmed for all GCs, this finding implies that the stellar source of the proton-capture element variations must not have produced significant amounts of Rb. This element is observed to be enhanced at extremely high levels in intermediate-mass asymptotic giant branch (IM-AGB) stars in the Magellanic Clouds (i.e., at a metallicity similar to 47 Tuc and NGC 6388). This fact may present a challenge to this popular candidate polluter, unless the mass range of the observed IM-AGB stars does not participate in the formation of the second-generation stars in GCs. A number of possible solutions are available to resolve this conundrum, including the fact that the Magellanic Cloud observations are very uncertain and may need to be revised. The fast rotating massive stars scenario would not face this potential problem as the slow mechanical winds of these stars during their main-sequence phase do not carry any Rb enhancements; however, these candidates face even bigger issues such as the production of Li and the close overlap with core-collapse supernova timescales. Observations of Sr, Rb, and Zr in metal-rich clusters such as NGC 6388 and NGC 6441 are sorely needed to clarify the situation.« less

  5. Ca2+ binding and conformational changes in a calmodulin domain.

    PubMed

    Evenäs, J; Malmendal, A; Thulin, E; Carlström, G; Forsén, S

    1998-09-29

    Calcium activation of the C-terminal domain of calmodulin was studied using 1H and 15N NMR spectroscopy. The important role played by the conserved bidentate glutamate Ca2+ ligand in the binding loops is emphasized by the striking effects resulting from a mutation of this glutamic acid to a glutamine, i.e. E104Q in loop III and E140Q in loop IV. The study involves determination of Ca2+ binding constants, assignments, and structural characterizations of the apo, (Ca2+)1, and (Ca2+)2 states of the E104Q mutant and comparisons to the wild-type protein and the E140Q mutant [Evenäs et al. (1997) Biochemistry 36, 3448-3457]. NMR titration data show sequential Ca2+ binding in the E104Q mutant. The first Ca2+ binds to loop IV and the second to loop III, which is the order reverse to that observed for the E140Q mutant. In both mutants, the major structural changes occur upon Ca2+ binding to loop IV, which implies a different response to Ca2+ binding in the N- and C-terminal EF-hands. Spectral characteristics show that the (Ca2+)1 and (Ca2+)2 states of the E104Q mutant undergo global exchange on a 10-100 micros time scale between conformations seemingly similar to the closed and open structures of this domain in wild-type calmodulin, paralleling earlier observations for the (Ca2+)2 state of the E140Q mutant, indicating that both glutamic acid residues, E104 and E140, are required for stabilization of the open conformation in the (Ca2+)2 state. To verify that the NOE constraints cannot be fulfilled in a single structure, solution structures of the (Ca2+)2 state of the E104Q mutant are calculated. Within the ensemble of structures the precision is good. However, the clearly dynamic nature of the state, a large number of violated distance restraints, ill-defined secondary structural elements, and comparisons to the structures of calmodulin indicate that the ensemble does not provide a good picture of the (Ca2+)2 state of the E104Q mutant but rather represents the distance-averaged structure of at least two distinct different conformations.

  6. Comparative analysis of 3D data accuracy of single tooth and full dental arch captured by different intraoral and laboratory digital impression systems.

    PubMed

    Ryakhovskiy, A N; Kostyukova, V V

    2016-01-01

    The aim of this study was to compare the accuracy of digital impressions taken by different intraoral and laboratory scanners. For this purpose a synthetic jaw model with prepared tooth was scanned using intraoral scanning systems: 3D Progress (MHT S.P.A., IT - MHT Optic Research AG, CH); True Definition (3M ESPE, USA); Trios (3Shape A/S, DNK); CEREC AC Bluecam, CEREC Omnicam (Sirona Dental System GmbH, DE); Planscan (Planmeca, FIN); and laboratory scanning systems: s600 ARTI (Zirkonzahn GmbH, IT); Imetric Iscan D104, CH); D900 (3Shape A/S, DNK); Zfx Evolution (Zfx GmbH, DE) (each n=10). Reference-scanning was done by ATOS Core (GOM mbH, DE). The resulting digital impressions were superimposed with the master-scan. The measured deviations by points (trueness) for intraoral scanners were: True Definition - 15.0±2.85 μm (single tooth) and 45.0±19.11 µm (full arch); Trios - 17.1±1.44 and 58.8±27.36 µm; CEREC AC Bluecam - 22.3±5.58 and 20.3±4.13 µm; CEREC Omnicam - 25.0±1.06 and 78.5±27.03 µm; 3D Progress - 26.4±5.75 and 213.5±47.44 µm; Planscan - 54.6±11.58 and 205.2±21.73 µm. For laboratory scanners: Imetric Iscan D104 - 10.2±0.87 μm (stamp) and 65.3±5.36 µm (full arch); Zfx Evolution - 12.8±0.83 and 66.4±2.80 µm; Zirkonzahn s600 ARTI - 15.1±1.36 and 65.9±1.33 µm; 3Shape D900 - 19.9±0.53 and 63.6±0.83 µm. Precision was: True Definition - 19.9±2.77 μm (single tooth) and 40.1±11.04 µm (full arch); Trios - 25.8±2.49 and 69.9±18.95 µm; CEREC AC Bluecam - 36.4±2.78 and 46.6±3.44 µm; CEREC Omnicam - 37.6±3.29 and 76.2±13.36 µm; 3D Progress - 76.9±11.04 and 102.2±8.06 µm; Planscan - 74.3±6.58 and 93.9±15.32 µm. For laboratory scanners: Imetric Iscan D104 - 11.7±4.39 μm (stamp) and 31.2±5.58 µm (full arch); Zfx Evolution - 8.4±0.49 and 24.8±3.98 µm; Zirkonzahn s600 ARTI - 13.4±6.74 and 20.7±4.34 µm; 3Shape D900 - 10.4±0.93 and 17.8±0.62 µm. Whole deviation of the dental arch was: 3D Progress - 98.0±5.70 µm; True Definition - 47.1±9.61 µm; Trios - 59.6±18.77 µm; Omnicam - 77.8±8.79 µm; Planscan - 107.9±1.58 µm; Bluecam - 46.8±1.22 µm; Imetric - 36.4±1.62 µm; Zfx Evolution - 29.5±0.58 µm; S600 ARTI - 35.0±1.04 µm; 3Shape D900 - 32.7±0.29 µm. The results indicate that digital impressions provide enough accuracy for clinical application.

  7. Magnetic field sensor based on the Ampere's force using dual-polarization DBR fiber laser

    NASA Astrophysics Data System (ADS)

    Yao, Shuang; Zhang, Yang; Guan, Baiou

    2015-08-01

    A novel magnetic field sensor using distributed Bragg reflector (DBR) fiber laser by Ampere's force effect is proposed and experimentally demonstrated. The key sensing element, that is the dual-polarization DBR fiber laser, is fixed on the middle part of two copper plates which carry the current. Ampere's force is applied onto the coppers due to an external magnetic field generated by a DC solenoid. Thus, the lateral force from the coppers is converted to a corresponding beat frequency signal shift produced by the DBR laser. The electric current sensing is also realized by the same configuration and same principle simultaneously in an intuitive manner. Good agreement between the theory calculation and the experimental results is obtained, which shows a good linearity. This sensor's sensitivity to the magnetic field and to the electric current finally reaches ~258.92 kHz/mT and ~1.08727 MHz/A, respectively.

  8. Characterization of the hupSL promoter activity in Nostoc punctiforme ATCC 29133

    PubMed Central

    2009-01-01

    Background In cyanobacteria three enzymes are directly involved in the hydrogen metabolism; a nitrogenase that produces molecular hydrogen, H2, as a by-product of nitrogen fixation, an uptake hydrogenase that recaptures H2 and oxidize it, and a bidirectional hydrogenase that can both oxidize and produce H2.Nostoc punctiforme ATCC 29133 is a filamentous dinitrogen fixing cyanobacterium containing a nitrogenase and an uptake hydrogenase but no bidirectional hydrogenase. Generally, little is known about the transcriptional regulation of the cyanobacterial uptake hydrogenases. In this study gel shift assays showed that NtcA has a specific affinity to a region of the hupSL promoter containing a predicted NtcA binding site. The predicted NtcA binding site is centred at 258.5 bp upstream the transcription start point (tsp). To further investigate the hupSL promoter, truncated versions of the hupSL promoter were fused to either gfp or luxAB, encoding the reporter proteins Green Fluorescent Protein and Luciferase, respectively. Results Interestingly, all hupsSL promoter deletion constructs showed heterocyst specific expression. Unexpectedly the shortest promoter fragment, a fragment covering 57 bp upstream and 258 bp downstream the tsp, exhibited the highest promoter activity. Deletion of the NtcA binding site neither affected the expression to any larger extent nor the heterocyst specificity. Conclusion Obtained data suggest that the hupSL promoter in N. punctiforme is not strictly dependent on the upstream NtcA cis element and that the shortest promoter fragment (-57 to tsp) is enough for a high and heterocyst specific expression of hupSL. This is highly interesting because it indicates that the information that determines heterocyst specific gene expression might be confined to this short sequence or in the downstream untranslated leader sequence. PMID:19284581

  9. Draft genome sequence of a KPC-2-producing Klebsiella pneumoniae ST340 carrying blaCTX-M-15 and blaCTX-M-59 genes: a rich genome of mobile genetic elements and genes encoding antibiotic resistance.

    PubMed

    Casella, Tiago; de Morais, Andressa Batista Zequini; de Paula Barcelos, Diego Diniz; Tolentino, Fernanda Modesto; Cerdeira, Louise Teixeira; Bueno, Maria Fernanda Campagnari; Francisco, Gabriela Rodrigues; de Andrade, Leonardo Neves; da Costa Darini, Ana Lucia; de Oliveira Garcia, Doroti; Lincopan, Nilton; Nogueira, Mara Corrêa Lelles

    2018-06-01

    Klebsiella pneumoniae is considered an opportunistic pathogen and an important agent of nosocomial and community infections. It presents the ability to capture and harbour several antimicrobial resistance genes and, in this context, the extensive use of carbapenems to treat serious infections has been responsible for the selection of several resistance genes. This study reports the draft genome sequence of a KPC-2-producing K. pneumoniae strain (Kp10) simultaneously harbouring bla CTX-M-15 and bla CTX-M-59 genes isolated from urine culture of a patient with Parkinson's disease. Classical microbiological methods were applied to isolate and identify the strain, and PCR and sequencing were used to identify and characterise the genes and the genetic environment. Whole-genome sequencing (WGS) was performed using a Nextera XT DNA library and a NextSeq platform. WGS analysis revealed the presence of 5915 coding genes, 46 RNA-encoding genes and 255 pseudogenes. Kp10 belonged to sequence type 340 (ST340) of clonal complex 258 (CC258) and carried 20 transferable genes associated with antimicrobial resistance, comprising seven drug classes. Although the simultaneous presence of different bla CTX-M genes in the same strain is rarely reported, the bla KPC-2 , bla CTX-M-15 and bla CTX-M-59 genes were not associated with the same genetic mobile structure in Kp10. These results confirm the capacity of K. pneumoniae to harbour several antimicrobial resistance genes. Thus, this draft genome could help in future epidemiological studies regarding the dissemination of clinically relevant resistance genes. Copyright © 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

  10. A Novel Cell-Based Hybrid Acoustic Wave Biosensor with Impedimetric Sensing Capabilities

    PubMed Central

    Liu, Fei; Li, Fang; Nordin, Anis Nurashikin; Voiculescu, Ioana

    2013-01-01

    A novel multiparametric biosensor system based on living cells will be presented. The biosensor system includes two biosensing techniques on a single device: resonant frequency measurements and electric cell-substrate impedance sensing (ECIS). The multiparametric sensor system is based on the innovative use of the upper electrode of a quartz crystal microbalance (QCM) resonator as working electrode for the ECIS technique. The QCM acoustic wave sensor consists of a thin AT-cut quartz substrate with two gold electrodes on opposite sides. For integration of the QCM with the ECIS technique a semicircular counter electrode was fabricated near the upper electrode on the same side of the quartz crystal. Bovine aortic endothelial live cells (BAECs) were successfully cultured on this hybrid biosensor. Finite element modeling of the bulk acoustic wave resonator using COMSOL simulations was performed. Simultaneous gravimetric and impedimetric measurements performed over a period of time on the same cell culture were conducted to validate the device's sensitivity. The time necessary for the BAEC cells to attach and form a compact monolayer on the biosensor was 35∼45 minutes for 1.5 × 104 cells/cm2 BAECs; 60 minutes for 2.0 × 104 cells/cm2 BAECs; 70 minutes for 3.0 × 104 cells/cm2 BAECs; and 100 minutes for 5.0 × 104 cells/cm2 BAECs. It was demonstrated that this time is the same for both gravimetric and impedimetric measurements. This hybrid biosensor will be employed in the future for water toxicity detection. PMID:23459387

  11. Resident Ratings of Communication Skills Using the Kalamazoo Adapted Checklist.

    PubMed

    Porcerelli, John H; Brennan, Simone; Carty, Jennifer; Ziadni, Maisa; Markova, Tsveti

    2015-09-01

    The Kalamazoo Essential Elements Communication Checklist-Adapted (KEECC-A) is a well-regarded instrument for evaluating communication and interpersonal skills. To date, little research has been conducted that assesses the accuracy of resident self-ratings of their communication skills. To assess whether residents can accurately self-rate communication skills, using the KEECC-A, during an objective structured clinical examination (OSCE). A group of 104 residents from 8 specialties completed a multistation OSCE as part of an institutional communication skills curriculum conducted at a single institution. Standardized patients (SPs) and observers were trained in rating communication skills using the KEECC-A. Standardized patient ratings and resident self-ratings were completed immediately following each OSCE encounter, and trained observers rated archived videotapes of the encounters. Resident self-ratings and SP ratings using the KEECC-A were significantly correlated (r104  = 0.238, P = .02), as were resident self-ratings and observer ratings (r104  = 0.284, P = .004). The correlation between the SP ratings and observer (r104  = 0.378, P = .001) ratings were larger in magnitude, but not significantly different (P > .05) from resident/SP or resident/observer correlations. The results suggest that residents, with a modicum of training using the KEECC-A, can accurately rate their own communication and interpersonal skills during an OSCE. Using trained observers to rate resident communication skills provides a unique opportunity for evaluating SP and resident self-ratings. Our findings also lend further support for the reliability and validity of the KEECC-A.

  12. Microfabrication of low-loss lumped-element Josephson circuits for non-reciprocal and parametric devices

    NASA Astrophysics Data System (ADS)

    Cicak, Katarina; Lecocq, Florent; Ranzani, Leonardo; Peterson, Gabriel A.; Kotler, Shlomi; Teufel, John D.; Simmonds, Raymond W.; Aumentado, Jose

    Recent developments in coupled mode theory have opened the doors to new nonreciprocal amplification techniques that can be directly leveraged to produce high quantum efficiency in current measurements in microwave quantum information. However, taking advantage of these techniques requires flexible multi-mode circuit designs comprised of low-loss materials that can be implemented using common fabrication techniques. In this talk we discuss the design and fabrication of a new class of multi-pole lumped-element superconducting parametric amplifiers based on Nb/Al-AlOx/Nb Josephson junctions on silicon or sapphire. To reduce intrinsic loss in these circuits we utilize PECVD amorphous silicon as a low-loss dielectric (tanδ 5 ×10-4), resulting in nearly quantum-limited directional amplification.

  13. Are All Heat Loads Created Equal?

    PubMed

    Meade, Robert D; Kenny, Glen P

    2017-09-01

    We evaluated physiological responses during exercise at a fixed evaporative requirement for heat balance (Ereq) but varying combinations of metabolic and environmental heat load. Nine healthy, physically active males (age: 46 ± 8 yr) performed four experimental sessions consisting of 75 min of semirecumbent cycling at various ambient temperatures. Whole-body dry heat loss (direct calorimetry) was monitored continuously as was heat production (indirect calorimetry), which was adjusted to achieve an Ereq of 400 W. The resultant metabolic heat productions and ambient temperatures for the sessions were as follows: (i) 440 W and 30°C (440 [30]), (ii) 388 W and 35°C (388 [35]), (iii) 317 W and 40°C (317 [40]), and (iv) 258 W and 45°C (258 [45]). Whole-body evaporative heat loss was determined via direct calorimetry. Esophageal (Tes) and mean skin (Tsk) temperatures as well as HR were monitored continuously. Mean body temperature (Tb) was calculated from Tes and Tsk. Physiological strain index (PSI) was determined from Tes and HR. End-exercise evaporative heat loss and Tb were similar between conditions (both P ≥ 0.48). Tes was greater in 440 [30] (37.67°C ± 0.04°C) and 388 [35] (37.58°C ± 0.07°C) relative to both 317 [40] (37.35°C ± 0.06°C) and 258 [45] (37.20°C ± 0.07°C; all P ≤ 0.05). Further, Tsk was different between each condition (440 [30], 33.85°C ± 0.16°C; 388 [35], 34.53°C ± 0.08°C; 317 [40], 35.67°C ± 0.07°C; and 258 [45], 36.54°C ± 0.08°C; all P < 0.01). In 440 [30], HR was elevated by about 13 and 18 bpm relative to 317 [40] and 258 [45], respectively (both P < 0.01). Finally, PSI was greater in both 440 [30] and 388 [35] compared with 317 [40] and 258 [45] (all P ≤ 0.04). Exercise at a fixed Ereq resulted in similar evaporative heat loss and Tb. However, the Tes, Tsk, HR, and PSI responses varied depending on the relative contribution of metabolic and environmental heat load.

  14. Measurement of the B0→π-ℓ+ν and B+→η(')ℓ+ν branching fractions, the B0→π-ℓ+ν and B+→ηℓ+ν form-factor shapes, and determination of |Vub|

    NASA Astrophysics Data System (ADS)

    Del Amo Sanchez, P.; Lees, J. P.; Poireau, V.; Prencipe, E.; Tisserand, V.; Garra Tico, J.; Grauges, E.; Martinelli, M.; Milanes, D. A.; Palano, A.; Pappagallo, M.; Eigen, G.; Stugu, B.; Sun, L.; Brown, D. N.; Kerth, L. T.; Kolomensky, Yu. G.; Lynch, G.; Osipenkov, I. L.; Koch, H.; Schroeder, T.; Asgeirsson, D. J.; Hearty, C.; Mattison, T. S.; McKenna, J. A.; Khan, A.; Randle-Conde, A.; Blinov, V. E.; Buzykaev, A. R.; Druzhinin, V. P.; Golubev, V. B.; Kravchenko, E. A.; Onuchin, A. P.; Serednyakov, S. I.; Skovpen, Yu. I.; Solodov, E. P.; Todyshev, K. Yu.; Yushkov, A. N.; Bondioli, M.; Curry, S.; Kirkby, D.; Lankford, A. J.; Mandelkern, M.; Martin, E. C.; Stoker, D. P.; Atmacan, H.; Gary, J. W.; Liu, F.; Long, O.; Vitug, G. M.; Campagnari, C.; Hong, T. M.; Kovalskyi, D.; Richman, J. D.; West, C.; Eisner, A. M.; Heusch, C. A.; Kroseberg, J.; Lockman, W. S.; Martinez, A. J.; Schalk, T.; Schumm, B. A.; Seiden, A.; Winstrom, L. O.; Cheng, C. H.; Doll, D. A.; Echenard, B.; Hitlin, D. G.; Ongmongkolkul, P.; Porter, F. C.; Rakitin, A. Y.; Andreassen, R.; Dubrovin, M. S.; Mancinelli, G.; Meadows, B. T.; Sokoloff, M. D.; Bloom, P. C.; Ford, W. T.; Gaz, A.; Nagel, M.; Nauenberg, U.; Smith, J. G.; Wagner, S. R.; Ayad, R.; Toki, W. H.; Jasper, H.; Karbach, T. M.; Petzold, A.; Spaan, B.; Kobel, M. J.; Schubert, K. R.; Schwierz, R.; Bernard, D.; Verderi, M.; Clark, P. J.; Playfer, S.; Watson, J. E.; Andreotti, M.; Bettoni, D.; Bozzi, C.; Calabrese, R.; Cecchi, A.; Cibinetto, G.; Fioravanti, E.; Franchini, P.; Luppi, E.; Munerato, M.; Negrini, M.; Petrella, A.; Piemontese, L.; Baldini-Ferroli, R.; Calcaterra, A.; de Sangro, R.; Finocchiaro, G.; Nicolaci, M.; Pacetti, S.; Patteri, P.; Peruzzi, I. M.; Piccolo, M.; Rama, M.; Zallo, A.; Contri, R.; Guido, E.; Lo Vetere, M.; Monge, M. R.; Passaggio, S.; Patrignani, C.; Robutti, E.; Tosi, S.; Bhuyan, B.; Prasad, V.; Lee, C. L.; Morii, M.; Adametz, A.; Marks, J.; Uwer, U.; Bernlochner, F. U.; Ebert, M.; Lacker, H. M.; Lueck, T.; Volk, A.; Dauncey, P. D.; Tibbetts, M.; Behera, P. K.; Mallik, U.; Chen, C.; Cochran, J.; Crawley, H. B.; Dong, L.; Meyer, W. T.; Prell, S.; Rosenberg, E. I.; Rubin, A. E.; Gritsan, A. V.; Guo, Z. J.; Arnaud, N.; Davier, M.; Derkach, D.; Firmino da Costa, J.; Grosdidier, G.; Le Diberder, F.; Lutz, A. M.; Malaescu, B.; Perez, A.; Roudeau, P.; Schune, M. H.; Serrano, J.; Sordini, V.; Stocchi, A.; Wang, L.; Wormser, G.; Lange, D. J.; Wright, D. M.; Bingham, I.; Chavez, C. A.; Coleman, J. P.; Fry, J. R.; Gabathuler, E.; Gamet, R.; Hutchcroft, D. E.; Payne, D. J.; Touramanis, C.; Bevan, A. J.; di Lodovico, F.; Sacco, R.; Sigamani, M.; Cowan, G.; Paramesvaran, S.; Wren, A. C.; Brown, D. N.; Davis, C. L.; Denig, A. G.; Fritsch, M.; Gradl, W.; Hafner, A.; Alwyn, K. E.; Bailey, D.; Barlow, R. J.; Jackson, G.; Lafferty, G. D.; Anderson, J.; Cenci, R.; Jawahery, A.; Roberts, D. A.; Simi, G.; Tuggle, J. M.; Dallapiccola, C.; Salvati, E.; Cowan, R.; Dujmic, D.; Sciolla, G.; Zhao, M.; Lindemann, D.; Patel, P. M.; Robertson, S. H.; Schram, M.; Biassoni, P.; Lazzaro, A.; Lombardo, V.; Palombo, F.; Stracka, S.; Cremaldi, L.; Godang, R.; Kroeger, R.; Sonnek, P.; Summers, D. J.; Nguyen, X.; Simard, M.; Taras, P.; de Nardo, G.; Monorchio, D.; Onorato, G.; Sciacca, C.; Raven, G.; Snoek, H. L.; Jessop, C. P.; Knoepfel, K. J.; Losecco, J. M.; Wang, W. F.; Corwin, L. A.; Honscheid, K.; Kass, R.; Morris, J. P.; Blount, N. L.; Brau, J.; Frey, R.; Igonkina, O.; Kolb, J. A.; Rahmat, R.; Sinev, N. B.; Strom, D.; Strube, J.; Torrence, E.; Castelli, G.; Feltresi, E.; Gagliardi, N.; Margoni, M.; Morandin, M.; Posocco, M.; Rotondo, M.; Simonetto, F.; Stroili, R.; Ben-Haim, E.; Bonneaud, G. R.; Briand, H.; Calderini, G.; Chauveau, J.; Hamon, O.; Leruste, Ph.; Marchiori, G.; Ocariz, J.; Prendki, J.; Sitt, S.; Biasini, M.; Manoni, E.; Rossi, A.; Angelini, C.; Batignani, G.; Bettarini, S.; Carpinelli, M.; Casarosa, G.; Cervelli, A.; Forti, F.; Giorgi, M. A.; Lusiani, A.; Neri, N.; Paoloni, E.; Rizzo, G.; Walsh, J. J.; Lopes Pegna, D.; Lu, C.; Olsen, J.; Smith, A. J. S.; Telnov, A. V.; Anulli, F.; Baracchini, E.; Cavoto, G.; Faccini, R.; Ferrarotto, F.; Ferroni, F.; Gaspero, M.; Li Gioi, L.; Mazzoni, M. A.; Piredda, G.; Renga, F.; Hartmann, T.; Leddig, T.; Schröder, H.; Waldi, R.; Adye, T.; Franek, B.; Olaiya, E. O.; Wilson, F. F.; Emery, S.; Hamel de Monchenault, G.; Vasseur, G.; Yèche, Ch.; Zito, M.; Allen, M. T.; Aston, D.; Bard, D. J.; Bartoldus, R.; Benitez, J. F.; Cartaro, C.; Convery, M. R.; Dorfan, J.; Dubois-Felsmann, G. P.; Dunwoodie, W.; Field, R. C.; Franco Sevilla, M.; Fulsom, B. G.; Gabareen, A. M.; Graham, M. T.; Grenier, P.; Hast, C.; Innes, W. R.; Kelsey, M. H.; Kim, H.; Kim, P.; Kocian, M. L.; Leith, D. W. G. S.; Li, S.; Lindquist, B.; Luitz, S.; Lynch, H. L.; Macfarlane, D. B.; Marsiske, H.; Muller, D. R.; Neal, H.; Nelson, S.; O'Grady, C. P.; Ofte, I.; Perl, M.; Pulliam, T.; Ratcliff, B. N.; Roodman, A.; Salnikov, A. A.; Santoro, V.; Schindler, R. H.; Schwiening, J.; Snyder, A.; Su, D.; Sullivan, M. K.; Sun, S.; Suzuki, K.; Thompson, J. M.; Va'Vra, J.; Wagner, A. P.; Weaver, M.; Wisniewski, W. J.; Wittgen, M.; Wright, D. H.; Wulsin, H. W.; Yarritu, A. K.; Young, C. C.; Ziegler, V.; Chen, X. R.; Park, W.; Purohit, M. V.; White, R. M.; Wilson, J. R.; Sekula, S. J.; Bellis, M.; Burchat, P. R.; Edwards, A. J.; Miyashita, T. S.; Ahmed, S.; Alam, M. S.; Ernst, J. A.; Pan, B.; Saeed, M. A.; Zain, S. B.; Guttman, N.; Soffer, A.; Lund, P.; Spanier, S. M.; Eckmann, R.; Ritchie, J. L.; Ruland, A. M.; Schilling, C. J.; Schwitters, R. F.; Wray, B. C.; Izen, J. M.; Lou, X. C.; Bianchi, F.; Gamba, D.; Pelliccioni, M.; Bomben, M.; Lanceri, L.; Vitale, L.; Lopez-March, N.; Martinez-Vidal, F.; Oyanguren, A.; Albert, J.; Banerjee, Sw.; Choi, H. H. F.; Hamano, K.; King, G. J.; Kowalewski, R.; Lewczuk, M. J.; Lindsay, C.; Nugent, I. M.; Roney, J. M.; Sobie, R. J.; Gershon, T. J.; Harrison, P. F.; Latham, T. E.; Puccio, E. M. T.; Band, H. R.; Dasu, S.; Flood, K. T.; Pan, Y.; Prepost, R.; Vuosalo, C. O.; Wu, S. L.

    2011-03-01

    We report the results of a study of the exclusive charmless semileptonic decays, B+→η(')ℓ+ν and B0→π-ℓ+ν, undertaken with approximately 464×106 BB¯ pairs collected at the Υ(4S) resonance with the BABAR detector. The analysis uses events in which the signal B decays are reconstructed with a loose neutrino reconstruction technique. We obtain partial branching fractions for B+→ηℓ+ν and B0→π-ℓ+ν decays in three and 12 bins of q2, respectively, from which we extract the f+(q2) form-factor shapes and the total branching fractions B(B+→ηℓ+ν)=(0.36±0.05stat±0.04syst)×10-4 and B(B0→π-ℓ+ν)=(1.42±0.05stat±0.07syst)×10-4. We also measure B(B+→η'ℓ+ν)=(0.24±0.08stat±0.03syst)×10-4. We obtain values for the magnitude of the CKM matrix element |Vub| using three different QCD calculations.

  15. Design and Construction of an Experimental Filament Winding Machine

    DTIC Science & Technology

    1993-05-01

    Mandrel to Heat Shield 102 H.3 Nusselt Geometric Analysis to Determine View Factor 104 H.4 Lamp Bank over Elemental Mandrel Area 105 J. 1 Mandrel inside...emissivity of the lamp bank Tj temperature of individual lamp in the lamp bank w, convective heat transfer coefficient N. Nusselt number Ro Reynolds...mandrel. The design intention was to allow cylinders to be formed by helical or hoop winding, or any variation in between. The type of winding is

  16. NASCAP-2K Version 4.2 Users Manual

    DTIC Science & Technology

    2014-10-31

    the surface element is metal or dielectric coating , the conductivity and capacitance (if a coating ), and the electron emission stimulated by incident...film covering an underlying conductor in meters. It is ignored for conductors . Bulk conductivity . This property is the bulk conductivity of the...surface material in ohms-1 m-1. A negative value indicates that the material is a conductor . If the bulk conductivity is in excess of 10-4 Ω-1 m-1, the

  17. Robust Littoral Characterization using Electro-Optical Sensors

    DTIC Science & Technology

    2011-09-30

    be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number . 1...Electro-Optical Sensors 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT... NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Orgeon State University,COAS,104 Ocean Admin Bldg,Corvallis,OR,97331-5503 8. PERFORMING

  18. Infiltration behaviour of elemental mercury DNAPL in fully and partially water saturated porous media

    NASA Astrophysics Data System (ADS)

    D'Aniello, Andrea; Hartog, Niels; Sweijen, Thomas; Pianese, Domenico

    2018-02-01

    Mercury is a contaminant of global concern due to its harmful effects on human health and for the detrimental consequences of its release in the environment. Sources of liquid elemental mercury are usually anthropogenic, such as chlor-alkali plants. To date insight into the infiltration behaviour of liquid elemental mercury in the subsurface is lacking, although this is critical for assessing both characterization and remediation approaches for mercury DNAPL contaminated sites. Therefore, in this study the infiltration behaviour of elemental mercury in fully and partially water saturated systems was investigated using column experiments. The properties affecting the constitutive relations governing the infiltration behaviour of liquid Hg0, and PCE for comparison, were determined using Pc(S) experiments with different granular porous media (glass beads and sands) for different two- and three-phase configurations. Results showed that, in water saturated porous media, elemental mercury, as PCE, acted as a non-wetting fluid. The required entry head for elemental mercury was higher (from about 5 to 7 times). However, due to the almost tenfold higher density of mercury, the required NAPL entry heads of 6.19 cm and 12.51 cm for mercury to infiltrate were 37.5% to 20.7% lower than for PCE for the same porous media. Although Leverett scaling was able to reproduce the natural tendency of Hg0 to be more prone than PCE to infiltrate in water saturated porous media, it considerably underestimated Hg0 infiltration capacity in comparison with the experimental results. In the partially water saturated system, in contrast with PCE, elemental mercury also acted as a nonwetting fluid, therefore having to overcome an entry head to infiltrate. The required Hg0 entry heads (10.45 and 15.74 cm) were considerably higher (68.9% and 25.8%) than for the water saturated porous systems. Furthermore, in the partially water saturated systems, experiments showed that elemental mercury displaced both air and water, depending on the initial water distribution within the pores. This indicates that the conventional wettability hierarchy, in which the NAPL has an intermediate wetting state between the air and the water phases, is not valid for liquid elemental mercury. Therefore, for future modelling of elemental mercury DNAPL infiltration behaviour in variably water saturated porous media, a different formulation of the governing constitutive relations will be required.

  19. Multiple animal studies for medical chemical defense program in soldier/patient decontamination and drug development on task order 86-25: Evaluation of the effectiveness of two ROHM and HAAS candidate decontamination systems against percutaneous application of undiluted TGD, Gd, VX HD, and L on the laboratory albino rabbit. Final report, 1 September 1986-1 February 1987

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Joiner, R.L.; Keys, W.B.; Harroff, H.H.

    1988-02-18

    A task was assigned to Battelle's Medical Research and Evaluation Facility(MREF) to evaluate the effectiveness of two candidate decontamination systems when compared to the standard dual component M258A1 decontamination system currently fielded by the U.S. Army. The chemical surety material (CSM) used in the evaluation were the organophosphates Soman (GD), polymer thickened GD (TGD), and VX, and the vesicants sulfur mustard (HD) and Lewisite (LS). The efficacies of the two candidate decontamination systems were evaluated in such a manner as to determine the LD50 and protective ratio (PR) for each decontaminant against each organophosphate CSM as compared to the standardmore » M258A1 decontamination system LD50. The PR constituted a comparison for each candidate system against the M258A1 standard. In the vesicant phase of the screen, the efficacies of the candidate systems were evaluated in a side-by-side comparison to the M258A1 decontamination system to determine whether the candidates were as good as or better than the standard dual component system.« less

  20. Emergence of Carbapenemase Producing Klebsiella Pneumonia and Spread of KPC-2 and KPC-17 in Taiwan: A Nationwide Study from 2011 to 2013.

    PubMed

    Tseng, I-Ling; Liu, Yu-Mei; Wang, Shiow-Jen; Yeh, Hung-Yi; Hsieh, Chia-Lun; Lu, Hsueh-Lin; Tseng, Yu-Chuan; Mu, Jung-Jung

    2015-01-01

    The emergence of carbapenemase-producing Klebsiella pneumoniae (CPKP) has become a great concern worldwide. In this study, 994 non-duplicate, carbapenem non-susceptible Klebsiella pneumonia isolates were collected in Taiwan from 2011 to 2013 for detection of the carbapenemase genes, assessment of antimicrobial susceptibility and molecular epidemiology studies. Of these 994 isolates, 183 (18.4%) had carbapenemase genes: 157 (15.8%) KPC (145 KPC-2 and 12 KPC-17), 16 (1.6%) IMP-8, 9 (0.9%) VIM-1, and 1 (0.1%) NDM-1. KPC had the highest prevalence rate among the carbapenemases and represented a major epidemic clone circulating in Taiwan. The ST512 and ST258 KPC-2 KPs were first identified in Taiwan and were grouped into a small cluster in the PFGE profile. In addition, the genetic structure encompassing the blaKPC gene of the ST512 and ST258 isolates showed a different pattern from that of other KPC isolates. ST11 may be a major sequence type circulating in Taiwan, although a specific minor clone has begun to be observed. This is the first report of ST258 and ST512 KPC-2 KP isolates in Taiwan, whether ST258 and ST512 will become the next endemic problems in Taiwan should be closely monitored.

  1. Genetic exchange between endogenous and exogenous LINE-1 repetitive elements in mouse cells.

    PubMed Central

    Belmaaza, A; Wallenburg, J C; Brouillette, S; Gusew, N; Chartrand, P

    1990-01-01

    The repetitive LINE (L1) elements of the mouse, which are present at about 10(5) copies per genome and share over 80% of sequence homology, were examined for their ability to undergo genetic exchange with exogenous L1 sequences. The exogenous L1 sequences, carried by a shuttle vector, consisted of an internal fragment from L1Md-A2, a previously described member of the L1 family of the mouse. Using an assay that does not require the reconstitution of a selectable marker we found that this vector, in either circular or linear form, acquired DNA sequences from endogenous L1 elements at a frequency of 10(-3) to 10(-4) per rescued vector. Physical analysis of the acquired L1 sequences revealed that distinct endogenous L1 elements acted as donors and that different subfamilies participated. These results demonstrate that L1 elements are readily capable of genetic exchange. Apart from gene conversion events, the acquisition of L1 sequences outside the region of homology suggested that a second mechanism was also involved in the genetic exchange. A model which accounts for this mechanism is presented and its potential implication on the rearrangement of L1 elements is discussed. Images PMID:1978749

  2. Experimental determination of carbon partitioning between upper mantle minerals and silicate melts: initial results and comparison to trace element partitioning (Nb, Rb, Ba, U, Th, K)

    NASA Astrophysics Data System (ADS)

    Rosenthal, A.; Hauri, E. H.; Hirschmann, M. M.; Davis, F. A.; Withers, A. C.; Fogel, M. L.

    2012-12-01

    Inventories of C in the mantle and magmatic fluxes of C between the mantle and the Earth's outer envelopes are poorly constrained in part owing to challenges in determining undegassed C concentrations of pristine basalts. Saal et al. [1] proposed that the behavior of Nb could be used as a proxy for C, owing to apparently similar behavior of the two elements in Siqueiros Transform MORB, but higher C/Nb ratios in popping rocks [2] call into question the applicability of the C/Nb proxy. Here, we present experimentally determined carbon partition coefficients (D's) between nominally volatile-free mantle minerals (olivine, OL; orthopyroxene, OPX; clinopyroxene, CPX; garnet, GA) and melts at 0.8-3 GPa, and 1250-1500°C. We conducted piston-cylinder experiments using an olivine-tholeiite + 4 wt% CO2, doped with Nb, Rb, U, Th, and 13C to enhance detection limits. To promote growth of crystals big enough for SIMS analyses, experiments were either long (<6 days), or at an initial higher temperature (T) before cooling slowly to a target T. We also produced SIMS calibration glass standards with varying amounts of C, and subject to ongoing analyses. We analyzed carbon (12C, 13C), H, F, and trace elements (Nb, Rb, Ba, U, Th, K) of both mineral phases and quenched liquids in subsets of experimental runs (21 in graphite-lined Pt-capsules, 6 in Fe-doped Pt-lined capsules) using both Cameca IMS 6F and NanoSIMS instruments. D's measured for 12C and 13C are close to 5x10-4, in most cases D13C>D12C, but a few have the opposite. Continuous exchange of the liquid (initially rich in 13C) with the graphite capsules (rich in 12C) may yield D's with 13C>12C. D's with 12C>13C are likely owing to either low count rates or comparatively high analytical contamination. Concentrations in minerals vary from 0.20-3.46 ppm for C, 25-176 ppm for H2O, and 0.05-1.21 ppm for F, whereas liquids tend to much higher values (C≤0.9 wt%; H2O≤1.5 wt%; F≤34 ppm; P≤0.25 wt%; S≤43 ppm; Cl≤77 ppm). Resulting D's indicate that C is highly incompatible in all major mantle mineral phases, with D's for OL, OPX and CPX of close to 5x10-4, and for GA ~2.2x10-4. D's for H2O (2x10-4 to ~3x10-2) and F (~2.3x10-3 to ~5.8x10-2) are comparable to those found in previous studies. Trace element partition determinations are in progress, but comparison to previous studies indicates that carbon is significantly more incompatible during mantle melting than Nb, U, or Th, and has behavior approximately similar to Ba. We therefore suggest that undegassed C/Ba ratios may be useful indicators of C fluxes and concentrations in basalt source regions where very low degrees of melting might fractionate C/Nb ratios. [1] Saal, A, Hauri, EH, Langmuir, CH, Perfit, M (2002) Nature 419, 451-455. [2] Cartigny, P, Pineau, F, Aubaud, C, Javoy, M (2008) Earth Planet Sci Lett 265, 672-685.

  3. Klebsiella pneumoniae Carbapenemase (KPC)-Producing K. pneumoniae at a Single Institution: Insights into Endemicity from Whole-Genome Sequencing

    PubMed Central

    Stoesser, Nicole; Sheppard, Anna E.; Pankhurst, Louise; Giess, Adam; Yeh, Anthony J.; Didelot, Xavier; Turner, Stephen D.; Sebra, Robert; Kasarskis, Andrew; Peto, Tim; Crook, Derrick; Sifri, Costi D.

    2015-01-01

    The global emergence of Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) multilocus sequence type ST258 is widely recognized. Less is known about the molecular and epidemiological details of non-ST258 K. pneumoniae in the setting of an outbreak mediated by an endemic plasmid. We describe the interplay of blaKPC plasmids and K. pneumoniae strains and their relationship to the location of acquisition in a U.S. health care institution. Whole-genome sequencing (WGS) analysis was applied to KPC-Kp clinical isolates collected from a single institution over 5 years following the introduction of blaKPC in August 2007, as well as two plasmid transformants. KPC-Kp from 37 patients yielded 16 distinct sequence types (STs). Two novel conjugative blaKPC plasmids (pKPC_UVA01 and pKPC_UVA02), carried by the hospital index case, accounted for the presence of blaKPC in 21/37 (57%) subsequent cases. Thirteen (35%) isolates represented an emergent lineage, ST941, which contained pKPC_UVA01 in 5/13 (38%) and pKPC_UVA02 in 6/13 (46%) cases. Seven (19%) isolates were the epidemic KPC-Kp strain, ST258, mostly imported from elsewhere and not carrying pKPC_UVA01 or pKPC_UVA02. Using WGS-based analysis of clinical isolates and plasmid transformants, we demonstrate the unexpected dispersal of blaKPC to many non-ST258 lineages in a hospital through spread of at least two novel blaKPC plasmids. In contrast, ST258 KPC-Kp was imported into the institution on numerous occasions, with other blaKPC plasmid vectors and without sustained transmission. Instead, a newly recognized KPC-Kp strain, ST941, became associated with both novel blaKPC plasmids and spread locally, making it a future candidate for clinical persistence and dissemination. PMID:25561339

  4. Volatilization, transport and sublimation of metallic and non-metallic elements in high temperature gases at Merapi Volcano, Indonesia

    USGS Publications Warehouse

    Symonds, R.B.; Rose, William I.; Reed, M.H.; Lichte, F.E.; Finnegan, David L.

    1987-01-01

    Condensates, silica tube sublimates and incrustations were sampled from 500-800??C fumaroles and lava samples were collected at Merapi Volcano, Indonesia in Jan.-Feb., 1984. With respect to the magma, Merapi gases are enriched by factors greater than 105 in Se, Re, Bi and Cd; 104-105 in Au, Br, In, Pb and W; 103-104 in Mo, Cl, Cs, S, Sn and Ag; 102-103 in As, Zn, F and Rb; and 1-102 in Cu, K, Na, Sb, Ni, Ga, V, Fe, Mn and Li. The fumaroles are transporting more than 106 grams/day ( g d) of S, Cl and F; 104-106 g/d of Al, Br, Zn, Fe, K and Mg; 103-104 g d of Pb, As, Mo, Mn, V, W and Sr; and less than 103 g d of Ni, Cu, Cr, Ga, Sb, Bi, Cd, Li, Co and U. With decreasing temperature (800-500??C) there were five sublimate zones found in silica tubes: 1) cristobalite and magnetite (first deposition of Si, Fe and Al); 2) K-Ca sulfate, acmite, halite, sylvite and pyrite (maximum deposition of Cl, Na, K, Si, S, Fe, Mo, Br, Al, Rb, Cs, Mn, W, P, Ca, Re, Ag, Au and Co); 3) aphthitalite (K-Na sulfate), sphalerite, galena and Cs-K. sulfate (maximum deposition of Zn, Bi, Cd, Se and In; higher deposition of Pb and Sn); 4) Pb-K chloride and Na-K-Fe sulfate (maximum deposition of Pb, Sn and Cu); and 5) Zn, Cu and K-Pb sulfates (maximum deposition of Pb, Sn, Ti, As and Sb). The incrustations surrounding the fumaroles are also chemically zoned. Bi, Cd, Pb, W, Mo, Zn, Cu, K, Na, V, Fe and Mn are concentrated most in or very close to the vent as expected with cooling, atmospheric contamination and dispersion. The highly volatile elements Br, Cl, As and Sb are transported primarily away from high temperature vents. Ba, Si, P, Al, Ca and Cr are derived from wall rock reactions. Incomplete degassing of shallow magma at 915??C is the origin of most of the elements in the Merapi volcanic gas, although it is partly contaminated by particles or wall rock reactions. The metals are transported predominantly as chloride species. As the gas cools in the fumarolic environment, it becomes saturated with sublimate phases that fractionate from the gas in the order of their equilibrium saturation temperatures. Devolatilization of a cooling batholith could transport enough acids and metals to a hydrothermal system to play a significant role in forming an ore deposit. However, sublimation from a high temperature, high velocity carrier gas is not efficient enough to form a large ore deposit. Re, Se, Cd and Bi could be used as supporting evidence for magmatic fluid transport in an ore deposit. ?? 1987.

  5. Ocean Engineering Studies Compiled 1991. Volume 11. Pressure-Resistant Glass Light Enclosures

    DTIC Science & Technology

    1991-01-01

    resting on this gasket than when they were resting on bare type 316 stainless steel end closures, aluminum gasket, or glass fiber- phenolic laminate ...316 2.58 inches thick Brass (naval) 2.58 inches thick Titanium (Ti-6AI-4V) 1.82 inches thick Phenolic resin-glass fiber laminate 4.40 inches thick...The 6061-T6 aluminum and the 94 spcimen s phenolic resin-impregnated glass fiber 18,000 imploded at -- /- laminate materials performed the best 18,000

  6. First Isolate of KPC-2-Producing Klebsiella pneumonaie Sequence Type 23 from the Americas

    PubMed Central

    Cejas, Daniela; Fernández Canigia, Liliana; Rincón Cruz, Giovanna; Elena, Alan X.; Maldonado, Ivana; Gutkind, Gabriel O.

    2014-01-01

    KPC-2-producing Klebsiella pneumoniae isolates mainly correspond to clonal complex 258 (CC258); however, we describe KPC-2-producing K. pneumoniae isolates belonging to invasive sequence type 23 (ST23). KPC-2 has scarcely been reported to occur in ST23, and this report describes the first isolation of this pathogen in the Americas. Acquisition of resistant markers in virulent clones could mark an evolutionary step toward the establishment of these clones as major nosocomial pathogens. PMID:25031447

  7. The Gd-Co-Al system at 870/1070 K as a representative of the rare earth-Co-Al family and new rare-earth cobalt aluminides: Crystal structure and magnetic properties

    NASA Astrophysics Data System (ADS)

    Morozkin, A. V.; Garshev, A. V.; Knotko, A. V.; Yapaskurt, V. O.; Mozharivskyj, Y.; Yuan, Fang; Yao, Jinlei; Nirmala, R.; Quezado, S.; Malik, S. K.

    2018-05-01

    The Gd-Co-Al system has been investigated at 870/1070 K by X-ray and elemental EDS analyses. The existence of the known compounds Gd2Co3Al9 (Y2Co3Ga9-type), Gd3Co4.5Al11.5 (Gd3Co4.6Al11) (Gd3Ru4Al12-type), Gd3Co6-7.4Al3-1.6 (CeNi3-type), GdCo1.15-0.65Al0.85-1.35 (MgZn2-type), Gd2Co2Al (Mo2NiB2-type) and Gd3Co3.5-3.25Al0.5-0.75 (W3CoB3-type) has been confirmed at 870/1070 K. Structure types have been determined for Gd2Co6Al19 (U2Co6Al19-type), Gd7Co6Al7 (Pr7Co6Al7-type), Gd6Co2-2.21Al1-0.79 (Ho6Co2Ga-type) and Gd14Co3.2Al2.8 (Gd14Co2.58Al3.42 at 970 K) (Lu14Co3In3-type). The structures of Gd6Co2Al, Gd6Co2.21Al0.79 and Gd14Co2.58Al3.42 flux-grown at 970 K have been refined from the single crystal X-ray diffraction data. Additionally, new ternary compounds Gd2Co5.7-5.3Al1.3-1.7 (Er2Co7-type) and Gd58Co20Al22 (unknown type structure) have been identified. Quasi-binary solid solutions were detected for Gd2Co17, GdCo5, Gd2Co7, GdCo3, GdCo2 and GdAl2 at 870/1070 K, while no appreciable solubility was observed for the other binary compounds in the Gd-Co-Al system. Magnetic properties of the Gd2Co3Al9, Gd3Co4.6Al11, Gd7Co6Al7, Gd6Co2.2Al0.8 and Gd14Co2.58Al3.42 compounds have been studied and are presented in this work. Gd6Co2.2Al0.8, Gd3Co4.6Al11, Gd7Co6Al7 and Gd14Co2.58Al3.42 order ferromagnetically, while Gd2Co3Al9 displays antiferromagnetic transition. Additionally, {Y, Sm, Tb - Tm}2Co6Al19 (U2Co6Al19-type), Yb2Co3Al9 (Y2Co3Ga9-type), {Y, Sm, Tm, Yb}3Co4.6Al11 (Gd3Ru4Al12-type) and Tb7Co6Al7 (Pr7Co6Al7-type) compounds have been synthesized and investigated.

  8. Finite element structural redesign by large admissible perturbations

    NASA Technical Reports Server (NTRS)

    Bernitsas, Michael M.; Beyko, E.; Rim, C. W.; Alzahabi, B.

    1991-01-01

    In structural redesign, two structural states are involved; the baseline (known) State S1 with unacceptable performance, and the objective (unknown) State S2 with given performance specifications. The difference between the two states in performance and design variables may be as high as 100 percent or more depending on the scale of the structure. A Perturbation Approach to Redesign (PAR) is presented to relate any two structural states S1 and S2 that are modeled by the same finite element model and represented by different values of the design variables. General perturbation equations are derived expressing implicitly the natural frequencies, dynamic modes, static deflections, static stresses, Euler buckling loads, and buckling modes of the objective S2 in terms of its performance specifications, and S1 data and Finite Element Analysis (FEA) results. Large Admissible Perturbation (LEAP) algorithms are implemented in code RESTRUCT to define the objective S2 incrementally without trial and error by postprocessing FEA results of S1 with no additional FEAs. Systematic numerical applications in redesign of a 10 element 48 degree of freedom (dof) beam, a 104 element 192 dof offshore tower, a 64 element 216 dof plate, and a 144 element 896 dof cylindrical shell show the accuracy, efficiency, and potential of PAR to find an objective state that may differ 100 percent from the baseline design.

  9. Perception and Practice of Road Safety among Medical Students, Mansoura, Egypt.

    PubMed

    Helal, Randah; El-Khawaga, Ghada; El-Gilany, Abdel-Hady

    2018-01-01

    To assess the knowledge and attitude of medical students towards road safety and to determine their driving behavior and its relation to different related factors. This cross-sectional study involved 480 medical students at Mansoura University, Egypt. A self-administered questionnaire was used to collect student personal data, knowledge about road safety, attitude towards road safety, and driving practices. More than 40% of students experienced an injury in the previous year, mainly as a pedestrian (56%), and 15.2% practiced driving, although only 9.6% had a driving licence. Most of the students had correct road safety knowledge except for awareness that the safe time to read maps is when your vehicle is parked (44%), one should drive in the left lane (29.6%), and one should overtake from the right-hand lane only (25.8%). The majority of the students reported that road traffic injuries can be prevented (89.2%). The mean score of the driving practices of the students ranged from 0.66±1.04 to 2.44±6.28 and rural residents showed significantly higher score regarding errors and lapses. Good road safety knowledge and a favorable, low risk attitude, did not translate into improved road traffic behavior and this highlights the importance of stricter implementation of the existing rules and including road safety in medical education programs.

  10. Norplant: users' perspective in Pakistan.

    PubMed

    Rehan, N; Inayatullah, A; Chaudhary, I

    1999-01-01

    Five hundred and eighteen Norplant acceptors (260 ever-users and 258 current users) were interviewed to assess their perceptions about Norplant. The mean age of the acceptors was 32.6+/-5.7 years (mean +/- SD). The mean parity was 4.3 and many of the users (40.2%) were illiterate. The most common reason to choose Norplant was its long duration of action (70.1%) followed by doctor's advice (10.4%) and use by other women (10.1%). Norplant was recommended by family planning workers in 35.3% cases, doctors in 29.2% cases and friends in 17.4% cases. Advertisement did not play any role in the women's choice of Norplant. In 77.3% cases, the decision to use Norplant was a joint decision. Only 15% of the users had fears/anxieties before insertion. Most of these women (44%) were concerned about possible ill-effects of Norplant on their health rather than efficacy. The social acceptance of Norplant was very high (76%) and more than half of the users (52.5%) were satisfied with the method. Among current users, 83.9% wanted to continue Norplant for 5 years. Only 39 users (15.1%) intended to discontinue. The main reason for discontinuation was menstrual disturbance (69.2%), followed by weight gain (12.7%). The study suggests that long duration of effective action and high social acceptance are likely to make Norplant a popular method among Pakistani women.

  11. [A method for identifying people with a high level of frailty by using a population database, Varese, Italy].

    PubMed

    Pisani, Salvatore; Gambino, Maria; Balconi, Lorena; Degli Stefani, Cristina; Speziali, Sabina; Bonarrigo, Domenico

    2016-01-01

    Since over 10 years, the Lombardy Region (Italy) has developed a system for classifying all persons registered with the healthcare system (database of persons registered with a general practitioner), according to their use of major healthcare services (hospitalizations, outpatient consultations, pharmaceutical) and whether they are exempt from copayment fees for disease-specific medications and healthcare services. The present study was conducted by the local health authorities of the province of Varese (Lombardy region, Italy) with 894.039 persons registered in the database of whom 258.770 (28.9%) with at least one chronic condition, 104.731 (11.7%) with multiple chronic conditions and 195.296 (21.8%) elderly persons. The aim was to evaluate death rates in different subgroups of patients entered in the database, including persons with chronic diseases and elderly persons. Standardized mortality rates were calculated for the year 2012. Compared with the general population, relative risk for mortality was 4,1 (95% confidence Intervals 4,0-4,2) in the elderly and 1,3 (95% confidence intervals 1,3-1,4) in chronic patients. This confirms that elderly persons have a higher level of frailty with respect to patients with chronic conditions. Mortality was found to be 28 times higher in elderly persons over 74 years of age, affected by high cost conditions such as cancer and cardiac disease, with respect to the general population.

  12. Teacher beliefs and cultural models: A challenge for science teacher preparation programs

    NASA Astrophysics Data System (ADS)

    Bryan, Lynn A.; Atwater, Mary M.

    2002-11-01

    The purpose of this paper is to present an argument for developing science teacher education programs that examine teachers' beliefs about multicultural issues and their impact on science teaching and learning. In the paper, we (a) delineate a rationale for the study of teacher beliefs about issues of culture and its impact on science teaching and learning; (b) assert three major categories of teacher beliefs to examine for designing teacher education programs that aim to meet the challenges of increasingly culturally diverse classrooms; and (c) discuss implications for science teacher education programs and research. Research has shown that knowing teachers' beliefs and designing instruction and experiences to explicitly confront those beliefs facilitate refinement of and/or transformation of beliefs and practices (Bryan & Abell, J Res Sci Teaching, 36, 121-140, 1999; Harrington & Hathaway, J Teacher Education, 46, 275-284, 1995; Hollingsworth, Am Educational Res J, 26(2), 160-189, 1989; Olmedo, J Teaching Teacher Education, 13, 245-258, 1997; Tobin & LaMaster, J Res Sci Teaching, 32, 225-242, 1995). Furthermore, prior to student teaching, preservice teachers need to be at least culturally sensitive teachers (Gillette, In Teacher Thinking in Cultural Contexts, F. A. Rios (Ed.); Albany, NY: State University of New York Press; 1996, pp. 104-128). Science educators need to continue to identify those beliefs and practices that undergird desirable and equitable science instruction.

  13. Possible role of Prussian blue nanoparticles in chemical evolution: interaction with ribose nucleotides

    NASA Astrophysics Data System (ADS)

    Sharma, Rachana; Iqubal, Md. Asif; Kamaluddin

    2016-01-01

    Ribonucleotides (RMPs) are the building blocks of genetic material consisting of a sugar group, a phosphate group and a nucleobase. Prussian blue (PB) is an ancient compound which is supposed to have formed under the conditions of primitive Earth. The interaction between nucleotides and mineral surfaces is of primary importance in the context of prebiotic chemistry. In the present work, the adsorption of RMPs on PB has been studied in the concentration range 0.4 × 10-4-3.0 × 10-4 M of RMPs at pH 7.5, T = 27°C and found to be 53.1, 41.7, 25.8 and 24.0% for adenosine 5'-monophosphate (5'-AMP), guanosine 5'-monophosphate, cytidine 5'-monophosphate and uridine 5'-monophosphate, respectively. Optimum conditions for the adsorption were studied as a function of concentration, time, amount of adsorbent and pH and data obtained were found to fit the Langmuir adsorption isotherm. Langmuir constants (K L and X m ) values were calculated. Fourier transform infrared spectroscopy, Raman spectroscopy, field-emission scanning electron microscopy and X-ray diffractometry techniques were used to investigate the interaction of RMPs on PB surface. Adsorption kinetics of 5'-AMP on PB has been found to be pseudo-second order. Results obtained from this study should prove valuable for a better understanding of the mechanism of RMP-PB interaction.

  14. Prevalence and Correlates of Hazardous Drinking among Female Sex Workers in 13 Mexican Cities

    PubMed Central

    Semple, Shirley J.; Pitpitan, Eileen V.; Chavarin, Claudia V.; Strathdee, Steffanie A.; Zavala, Rosa Icela; Aarons, Gregory A.; Patterson, Thomas L.

    2016-01-01

    Aims To describe the prevalence and correlates of hazardous drinking among female sex workers (FSWs) at 13 sites throughout Mexico. Methods FSWs (N = 1089) who were enrolled in a brief sexual risk reduction intervention (Mujer Segura) were queried about their sexual risk and substance use practices and their work contexts. Participants were classified as hazardous or non-hazardous drinkers based on the Alcohol Use Disorders test (AUDIT-C). Logistic regression models were used to examine individual, contextual, and community-level factors as correlates of hazardous drinking. Results Ninety-two percent of participants reported alcohol consumption in the past month. Among drinkers (N = 1001), 83% met AUDIT-C criteria for hazardous drinking. Factors that were independently associated with hazardous drinking included: drug use in the past month (adjusted odds ratio (AOR) = 3.31; 95% CI 1.29—8.45), being a cigarette smoker (AOR = 1.71; 95% CI 1.13—2.58), being a barmaid or dance hostess (AOR = 3.40; 95% CI 1.95–5.91), alcohol use before or during sex with clients (AOR = 7.78; 95% CI 4.84–12.52), and working in a city with a higher marginalization index (AOR = 1.07; 95% CI 1.04–1.11). Conclusions Findings support the high prioritization by public health authorities of alcohol prevention and treatment programs for FSWs. PMID:26546017

  15. Mammary Cancer and Activation of Transposable Elements

    DTIC Science & Technology

    2013-09-01

    formal monthly electronic lab meeting between Peaston lab and Edwards lab. And regularly hold meetings. • An informal schedule was set up with a plan...cytes and ADS-derived induced pluripotent stem cells (ADS-iPSCs) (19) and primary mouse ES cells to isolated sperm and oocytes (20). We selected an...051 59 5 92% H9-IMR90 5875 7 669 782 605 58 91% oocyte - ES cell (mouse) 4727 1 204 883 334 25 93% sperm - ES cell (mouse) 4580 4 364 748 1027 104 91

  16. Asteroid families - An initial search

    NASA Technical Reports Server (NTRS)

    Williams, James G.

    1992-01-01

    A stereo examination was conducted for clusters in three-dimensional proper element space within a sample of both numbered and faint Palomar-Leiden Survey (PLS) asteroids. The clusters were then objectively filtered for small Poisson probability of chance occurrence; 104 were accepted as families with 4- to 12-member populations, and are interpreted as impact-generated. Structure is common in the well-populated families: the better-sampled families are accordingly discussed in terms of their geometry and taxonomy. Some families are very rich in faint PLS members.

  17. Evaluating the Effectiveness of Various Blast Loading Descriptors as Occupant Injury Predictors for Underbody Blast Events

    DTIC Science & Technology

    2014-01-09

    of Hybrid III ATD LSDYNA model with FTSS v7.1.6 finite element dummy 6 Unclassified: Distribution Statement A. Approved for public release...descriptors as occupant injury predictors for underbody blast events Recording injury metrics Response from the dummy especially pelvic acceleration and...Ciip(H&ad CG,2) "’"’ "-......--------, I Max : 122.669 @59.81 7!; Time, ms Pelvic Z acceleration, g I I Clip: -4.75737 Ts:97.4138 Te: 104.414

  18. Uptake and cellular distribution, in four plant species, of fluorescently labeled mesoporous silica nanoparticles.

    PubMed

    Sun, Dequan; Hussain, Hashmath I; Yi, Zhifeng; Siegele, Rainer; Cresswell, Tom; Kong, Lingxue; Cahill, David M

    2014-08-01

    We report the uptake of MSNs into the roots and their movement to the aerial parts of four plant species and their quantification using fluorescence, TEM and proton-induced x - ray emission (micro - PIXE) elemental analysis. Monodispersed mesoporous silica nanoparticles (MSNs) of optimal size and configuration were synthesized for uptake by plant organs, tissues and cells. These monodispersed nanoparticles have a size of 20 nm with interconnected pores with an approximate diameter of 2.58 nm. There were no negative effects of MSNs on seed germination or when transported to different organs of the four plant species tested in this study. Most importantly, for the first time, a combination of confocal laser scanning microscopy, transmission electron microscopy and proton-induced X-ray emission (micro-PIXE) elemental analysis allowed the location and quantification MSNs in tissues and in cellular and sub-cellular locations. Our results show that MSNs penetrated into the roots via symplastic and apoplastic pathways and then via the conducting tissues of the xylem to the aerial parts of the plants including the stems and leaves. The translocation and widescale distribution of MSNs in plants will enable them to be used as a new delivery means for the transport of different sized biomolecules into plants.

  19. THE VARIABLE NEAR-INFRARED COUNTERPART OF THE MICROQUASAR GRS 1758–258

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Luque-Escamilla, Pedro L.; Martí, Josep; Muñoz-Arjonilla, Álvaro J., E-mail: peter@ujaen.es, E-mail: jmarti@ujaen.es, E-mail: ajmunoz@ujaen.es

    2014-12-10

    We present a new study of the microquasar system GRS 1758–258 in the near-infrared domain based on archival observations with the Hubble Space Telescope and the NICMOS camera. In addition to confirming the near-infrared counterpart pointed out by Muñoz-Arjonilla et al., we show that this object displays significant photometric variability. From its average magnitudes, we also find that GRS 1758–258 fits well within the correlation between the optical/near-infrared and X-ray luminosity known to exist for low-mass, black-hole candidate X-ray binaries in a hard state. Moreover, the spectral energy distribution built using all radio, near-infrared, and X-ray data available closest inmore » time to the NICMOS observations can be reasonably interpreted in terms of a self-absorbed radio jet and an irradiated accretion disk model around a stellar-mass black hole. All these facts match the expected behavior of a compact binary system and strengthen our confidence in the counterpart identification.« less

  20. Contamination and ecological risk assessment of toxic trace elements in the Xi River, an urban river of Shenyang city, China.

    PubMed

    Lin, Chunye; He, Mengchang; Liu, Xitao; Guo, Wei; Liu, Shaoqing

    2013-05-01

    The objectives of this study were to assess the enrichment, contamination, and ecological risk posed by toxic trace elements in the sediments of the Xi River in the industrialized city of Shenyang, China. Surface sediment and sediment core were collected; analyzed for toxic trace elements; and assessed with an index of geoaccumulation (Igeo), enrichment factor (EF) value, potential ecological risk factor (Er), ecological risk index (RI), and probable effect concentration quotient (PECQ). Elemental concentrations (milligram per kilogram) were 8.5-637.9 for As, 6.5-103.9 for Cd, 12.2-21.9 for Co, 90.6-516.0 for Cr, 258.1-1,791.5 for Cu, 2.6-19.0 for Hg, 70.5-174.5 for Ni, 126.9-1,405.8 for Pb, 3.7-260.0 for Sb, 38.4-100.4 for V, and 503-4,929 for Zn. The Igeo, EF, Er, and PECQ indices showed that the contamination of Cd and Hg was more serious than that of As, Cr, Cu, Ni, Pb, Sb, and Zn, whereas the presence of Co and V might be primarily from natural sources. The Igeo index for Cr and Ni might underestimate the degree of contamination, potentially as a result of high concentrations of these elements in the shale. The RI index was higher than 600, indicating a notably high ecological risk of sediment for the river. The average PECQ for As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn ranged from 1.4 to 4.1 for surface sediment and from 5.2 to 9.6 in the sediment cores, indicating a high potential for an adverse biological effect. It was concluded that the sediment in the Xi River was severely contaminated and should be remediated as a hazardous material.

  1. Normal modes of a superconducting transmission-line resonator with embedded lumped element circuit components

    NASA Astrophysics Data System (ADS)

    Mortensen, Henrik Lund; Mølmer, Klaus; Andersen, Christian Kraglund

    2016-11-01

    We present a method to identify the coupled, normal modes of a superconducting transmission line with an embedded lumped element circuit. We evaluate the effective transmission-line nonlinearities in the case of Kerr-like Josephson interactions in the circuit and in the case where the embedded circuit constitutes a qubit degree of freedom, which is Rabi coupled to the field in the transmission line. Our theory quantitatively accounts for the very high and positive Kerr nonlinearities observed in a recent experiment [M. Rehák, P. Neilinger, M. Grajcar, G. Oelsner, U. Hübner, E. Il'ichev, and H.-G. Meyer, Appl. Phys. Lett. 104, 162604 (2014), 10.1063/1.4873719], and we can evaluate the accomplishments of modified versions of the experimental circuit.

  2. Long-Term Multiwavelength Observations of GRS 1758-258 and the Advection-dominated Accretion Flow Model

    NASA Astrophysics Data System (ADS)

    Keck, John W.; Craig, William W.; Hailey, Charles J.; Harrison, Fiona; Hong, Jae Sub; Kahn, Steven M.; Lubin, Philip M.; McLean, Ryan; Pivovaroff, Michael J.; Seiffert, Michael; Wurtz, Ron; Ziock, Klaus P.

    2001-12-01

    We present a long-term multiwavelength light curve of Galactic black hole candidate GRS 1758-258 by combining previously published and archival data from Granat, ROSAT, the Compton Gamma Ray Observatory, the Rossi X-Ray Timing Explorer, BeppoSAX, ASCA, EXOSAT, and the Very Large Array. In addition, we include the first spectral results from the balloon-borne Gamma-Ray Arcminute Telescope Imaging System (GRATIS). In light of divergent analyses of the 1991-1993 ROSAT observations, we have reanalyzed these data; we find that the soft X-rays track the hard X-rays and that the fits require no blackbody component-indicating that GRS 1758-258 did not go to the high state in 1993. We offer an interpretation of these long-baseline data based on the advection-dominated accretion flow (ADAF) model for a system with m<~mcrit. We find that the 1990-1993 coeval hard and soft X-ray observations support the ADAF predictions. We discuss a new way to constrain black hole mass with spectral data and the ADAF theory and apply this technique to GRS 1758-258 to find M1>~8-9 Msolar at an assumed distance of 8.5 kpc. Further investigations of the ADAF model allow us to evaluate the model critically against the 1996 data and flux-flux diagram of Barret, McClintock, & Grindlay and to understand the limits of the latter's ``X-ray burster box.''

  3. Fine-scale mapping of the 4q24 locus identifies two independent loci associated with breast cancer risk.

    PubMed

    Guo, Xingyi; Long, Jirong; Zeng, Chenjie; Michailidou, Kyriaki; Ghoussaini, Maya; Bolla, Manjeet K; Wang, Qin; Milne, Roger L; Shu, Xiao-Ou; Cai, Qiuyin; Beesley, Jonathan; Kar, Siddhartha P; Andrulis, Irene L; Anton-Culver, Hoda; Arndt, Volker; Beckmann, Matthias W; Beeghly-Fadiel, Alicia; Benitez, Javier; Blot, William; Bogdanova, Natalia; Bojesen, Stig E; Brauch, Hiltrud; Brenner, Hermann; Brinton, Louise; Broeks, Annegien; Brüning, Thomas; Burwinkel, Barbara; Cai, Hui; Canisius, Sander; Chang-Claude, Jenny; Choi, Ji-Yeob; Couch, Fergus J; Cox, Angela; Cross, Simon S; Czene, Kamila; Darabi, Hatef; Devilee, Peter; Droit, Arnaud; Dörk, Thilo; Fasching, Peter A; Fletcher, Olivia; Flyger, Henrik; Fostira, Florentia; Gaborieau, Valerie; García-Closas, Montserrat; Giles, Graham G; Grip, Mervi; Guénel, Pascal; Haiman, Christopher A; Hamann, Ute; Hartman, Mikael; Hollestelle, Antoinette; Hopper, John L; Hsiung, Chia-Ni; Ito, Hidemi; Jakubowska, Anna; Johnson, Nichola; Kabisch, Maria; Kang, Daehee; Khan, Sofia; Knight, Julia A; Kosma, Veli-Matti; Lambrechts, Diether; Le Marchand, Loic; Li, Jingmei; Lindblom, Annika; Lophatananon, Artitaya; Lubinski, Jan; Mannermaa, Arto; Manoukian, Siranoush; Margolin, Sara; Marme, Frederik; Matsuo, Keitaro; McLean, Catriona A; Meindl, Alfons; Muir, Kenneth; Neuhausen, Susan L; Nevanlinna, Heli; Nord, Silje; Olson, Janet E; Orr, Nick; Peterlongo, Paolo; Putti, Thomas Choudary; Rudolph, Anja; Sangrajrang, Suleeporn; Sawyer, Elinor J; Schmidt, Marjanka K; Schmutzler, Rita K; Shen, Chen-Yang; Shi, Jiajun; Shrubsole, Martha J; Southey, Melissa C; Swerdlow, Anthony; Teo, Soo Hwang; Thienpont, Bernard; Toland, Amanda Ewart; Tollenaar, Robert A E M; Tomlinson, Ian P M; Truong, Thérèse; Tseng, Chiu-Chen; van den Ouweland, Ans; Wen, Wanqing; Winqvist, Robert; Wu, Anna; Yip, Cheng Har; Zamora, M Pilar; Zheng, Ying; Hall, Per; Pharoah, Paul D P; Simard, Jacques; Chenevix-Trench, Georgia; Dunning, Alison M; Easton, Douglas F; Zheng, Wei

    2015-11-01

    A recent association study identified a common variant (rs9790517) at 4q24 to be associated with breast cancer risk. Independent association signals and potential functional variants in this locus have not been explored. We conducted a fine-mapping analysis in 55,540 breast cancer cases and 51,168 controls from the Breast Cancer Association Consortium. Conditional analyses identified two independent association signals among women of European ancestry, represented by rs9790517 [conditional P = 2.51 × 10(-4); OR, 1.04; 95% confidence interval (CI), 1.02-1.07] and rs77928427 (P = 1.86 × 10(-4); OR, 1.04; 95% CI, 1.02-1.07). Functional annotation using data from the Encyclopedia of DNA Elements (ENCODE) project revealed two putative functional variants, rs62331150 and rs73838678 in linkage disequilibrium (LD) with rs9790517 (r(2) ≥ 0.90) residing in the active promoter or enhancer, respectively, of the nearest gene, TET2. Both variants are located in DNase I hypersensitivity and transcription factor-binding sites. Using data from both The Cancer Genome Atlas (TCGA) and Molecular Taxonomy of Breast Cancer International Consortium (METABRIC), we showed that rs62331150 was associated with level of expression of TET2 in breast normal and tumor tissue. Our study identified two independent association signals at 4q24 in relation to breast cancer risk and suggested that observed association in this locus may be mediated through the regulation of TET2. Fine-mapping study with large sample size warranted for identification of independent loci for breast cancer risk. ©2015 American Association for Cancer Research.

  4. Experimental data showing the thermal behavior of a flat roof with phase change material.

    PubMed

    Tokuç, Ayça; Başaran, Tahsin; Yesügey, S Cengiz

    2015-12-01

    The selection and configuration of building materials for optimal energy efficiency in a building require some assumptions and models for the thermal behavior of the utilized materials. Although the models for many materials can be considered acceptable for simulation and calculation purposes, the work for modeling the real time behavior of phase change materials is still under development. The data given in this article shows the thermal behavior of a flat roof element with a phase change material (PCM) layer. The temperature and energy given to and taken from the building element are reported. In addition the solid-liquid behavior of the PCM is tracked through images. The resulting thermal behavior of the phase change material is discussed and simulated in [1] A. Tokuç, T. Başaran, S.C. Yesügey, An experimental and numerical investigation on the use of phase change materials in building elements: the case of a flat roof in Istanbul, Build. Energy, vol. 102, 2015, pp. 91-104.

  5. Mineral content of Chinese medicinal herbs used as diuretic treatments for Taiwanese children.

    PubMed

    Chen, Chien-Yi

    2005-01-01

    Eighteen major, minor and trace elements in 12 Chinese medicinal herbs commonly consumed by Taiwanese Children as diuretics were determined by instrumental neutron activation analysis (INAA). Dried and powdered herb samples were irradiated in a neutron flux of ca. 2 x 10(12) n/cm2 s under separate short and long irradiation schemes. Lichen (IAEA-336) was used as the reference standard, and tomato leaves (NIST-SRM 1570a) were employed for cross-checking the accuracy of the results. INAA was shown to be a reliable multi-element analytical method for determining the content of both toxicologically and nutritionally important minerals in Chinese medicinal herbs. Determined elements were present in the dried herbs in concentrations ranging from 10(4) to 10(-3) microg/g. The mineral contents and the maximum daily intake values of the tested herbs were compared with published values and with the recommended daily intakes for Taiwanese children as specified by the World Health Organization.

  6. Pre-existing Pulmonary Diseases and Survival in Patients With Stage-dependent Lung Adenocarcinoma

    PubMed Central

    Jian, Zhi-Hong; Huang, Jing-Yang; Nfor, Oswald Ndi; Jhang, Kai-Ming; Ku, Wen-Yuan; Ho, Chien-Chang; Lung, Chia-Chi; Pan, Hui-Hsien; Liang, Yu-Chiu; Wu, Ming-Fang; Liaw, Yung-Po

    2016-01-01

    Abstract Asthma, chronic obstructive pulmonary disease (COPD), and pulmonary tuberculosis (TB) are common lung diseases associated with lung cancer mortality. This study evaluated sex disparities in pre-existing pulmonary diseases and stage-dependent lung adenocarcinoma survival. Patients newly diagnosed with lung adenocarcinoma between 2003 and 2008 were identified using the National Health Insurance Research Database and Cancer Registry. Cases with lung adenocarcinoma were followed until the end of 2010. Survival curves were estimated by the Kaplan–Meier method. Cox proportional-hazard regression was used to calculate the hazard ratio (HR) of pre-existing asthma, COPD, and/or TB, and to estimate all-cause mortality risk in patients with different stages of lung adenocarcinoma. A total of 14,518 cases were identified with lung adenocarcinoma. Specifically, among men, the HRs for TB were 1.69 (95% confidence interval [CI], 1.10–2.58), 1.48 (95% CI, 1.14–1.93), and 1.27 (95% CI, 1.08–1.49) for individuals with stage I + II, III, and IV diseases, respectively. The HRs for asthma were 1.41 (95% CI, 1.00–1.99) in women with stage I + II and 1.14 (95% CI, 1.04–1.26) in men with stage IV disease. For pulmonary disease combinations in men, the HRs were 1.45 (95% CI, 1.12–1.89) for asthma + COPD + TB, 1.35 (95% CI, 1.12–1.63) for COPD + TB, 1.28 (95% CI, 1.01–1.63) for TB, and 1.15 (95%CI, 1.04–1.27) for asthma + COPD, respectively. For women with stage I + II disease, the HR was 6.94 (95% CI, 2.72–17.71) for asthma + COPD + TB. Coexistence of pre-existing pulmonary diseases increased mortality risk in men with adenocarcinoma. TB is at elevated risk of mortality among men with different stages of adenocarcinoma. Asthmatic women with early-stage adenocarcinoma had increased risk of mortality. PMID:26962806

  7. Antenatal Iron Supplementation Regimens for Pregnant Women in Rural Vietnam and Subsequent Haemoglobin Concentration and Anaemia among Their Infants.

    PubMed

    Tran, Thach Duc; Fisher, Jane; Hanieh, Sarah; Tran, Tuan; Simpson, Julie Anne; Tran, Ha; Biggs, Beverley-Ann

    2015-01-01

    Little evidence about the effects of antenatal iron supplementation on infant anaemia is available. The aim was to compare effects on six-month-old infants' Haemoglobin (Hb) concentration and anaemia of daily iron-folic acid (IFA), twice-weekly IFA with or without other micronutrients (MMN) and usual antenatal care in rural Vietnam. Secondary data analysis from: a prospective population-based observational study (OS) which examined effects of antenatal psychosocial factors, anaemia and iron deficiency on infant development and health; and a three-arm cluster randomised trial (CRT) of different antenatal iron supplementation regimens. In the OS 497 women (<20 weeks gestation) from 50 randomly-selected communes participated, and in the CRT 1,258 pregnant women (<16 weeks gestation) in 104 communes were allocated randomly to trial arms. The main outcome was six-month-old infant Hb concentration. Baseline data included women's socio-demographic characteristics, reproductive health, Hb and serum ferritin. Mean differences in infant Hb and odds ratios of infant anaemia between CRT arms and OS were calculated by multivariable regression models, controlling for baseline differences and clustering, using robust standard errors. Infant anaemia prevalence was 68.6% in the OS, 47.2% daily IFA, 53.5% weekly IFA, and 50.3% MMN conditions. After adjustment, mean infant haemoglobin levels in daily IFA (mean difference = 0.95 g/dL; 95%CI 0.7-11.18); weekly IFA (0.91; 95%CI 0.69-1.12) and MMN (1.04; 95%CI 0.8-1.27) were higher than in the OS. After adjustment there were lower odds ratios of anaemia among infants in the daily IFA (OR = 0.31; 95% CI 0.22-0.43), weekly IFA (0.38; 95%CI 0.26-0.54) and MMN (0.33; 95%CI 0.23-0.48) groups than in the OS. Infant anaemia is a public health problem in Vietnam and other resource-constrained countries. All supplementation regimens could have clinically significant benefits for Hb and reduce anaemia risk among six-month-old infants. Universal provision of free intermittent iron supplements is warranted.

  8. Antenatal Iron Supplementation Regimens for Pregnant Women in Rural Vietnam and Subsequent Haemoglobin Concentration and Anaemia among Their Infants

    PubMed Central

    Tran, Thach Duc; Fisher, Jane; Hanieh, Sarah; Tran, Tuan; Simpson, Julie Anne; Tran, Ha; Biggs, Beverley-Ann

    2015-01-01

    Background Little evidence about the effects of antenatal iron supplementation on infant anaemia is available. The aim was to compare effects on six-month-old infants’ Haemoglobin (Hb) concentration and anaemia of daily iron–folic acid (IFA), twice-weekly IFA with or without other micronutrients (MMN) and usual antenatal care in rural Vietnam. Methods and Findings Secondary data analysis from: a prospective population-based observational study (OS) which examined effects of antenatal psychosocial factors, anaemia and iron deficiency on infant development and health; and a three-arm cluster randomised trial (CRT) of different antenatal iron supplementation regimens. In the OS 497 women (<20 weeks gestation) from 50 randomly-selected communes participated, and in the CRT 1,258 pregnant women (<16 weeks gestation) in 104 communes were allocated randomly to trial arms. The main outcome was six-month-old infant Hb concentration. Baseline data included women’s socio-demographic characteristics, reproductive health, Hb and serum ferritin. Mean differences in infant Hb and odds ratios of infant anaemia between CRT arms and OS were calculated by multivariable regression models, controlling for baseline differences and clustering, using robust standard errors. Infant anaemia prevalence was 68.6% in the OS, 47.2% daily IFA, 53.5% weekly IFA, and 50.3% MMN conditions. After adjustment, mean infant haemoglobin levels in daily IFA (mean difference = 0.95 g/dL; 95%CI 0.7-11.18); weekly IFA (0.91; 95%CI 0.69-1.12) and MMN (1.04; 95%CI 0.8-1.27) were higher than in the OS. After adjustment there were lower odds ratios of anaemia among infants in the daily IFA (OR = 0.31; 95% CI 0.22-0.43), weekly IFA (0.38; 95%CI 0.26-0.54) and MMN (0.33; 95%CI 0.23-0.48) groups than in the OS. Conclusions Infant anaemia is a public health problem in Vietnam and other resource-constrained countries. All supplementation regimens could have clinically significant benefits for Hb and reduce anaemia risk among six-month-old infants. Universal provision of free intermittent iron supplements is warranted. PMID:25928545

  9. Effect of milk thistle (Silybum marianum) on the pharmacokinetics of irinotecan.

    PubMed

    van Erp, Nielka P H; Baker, Sharyn D; Zhao, Ming; Rudek, Michelle A; Guchelaar, Henk-Jan; Nortier, Johan W R; Sparreboom, Alex; Gelderblom, Hans

    2005-11-01

    Milk thistle (Silybum marianum) is one of the most commonly used herbal therapies, and its principal constituent silybin significantly inhibits cytochrome P450 isoform 3A4 (CYP3A4) and UDP glucuronosyltransferase isoform 1A1 (UGT1A1) in vitro. Here, we investigated whether milk thistle affects the pharmacokinetics of irinotecan, a substrate for CYP3A4 and UGT1A1, in humans. Six cancer patients were treated with irinotecan (dose, 125 mg/m(2)) given as a 90-minute infusion once every week. Four days before the second dose, patients received 200 mg milk thistle, thrice a day, for 14 consecutive days. Pharmacokinetic studies of irinotecan and its metabolites 7-ethyl-10-hydroxycamptothecin (SN-38), 7-ethyl-10-[3,4,5-trihydroxy-pyran-2-carboxylic acid]-camptothecin (SN-38-glucuronide), and 7-ethyl-10-[4-N-(5-aminopentanoic acid)-1-piperidino]-carbonyloxycamptothecin were done during the first three irinotecan administrations. Short-term (4 days) or more prolonged intake of milk thistle (12 days) had no significant effect on irinotecan clearance (mean, 31.2 versus 25.4 versus 25.6 L/h; P = 0.16). The area under the curve ratio of SN-38 and irinotecan was slightly decreased by milk thistle (2.58% versus 2.23% versus 2.17%; P = 0.047), whereas the relative extent of glucuronidation of SN-38 was similar (10.8 versus 13.5 versus 13.1; P = 0.64). Likewise, the area under the curve ratio of 7-ethyl-10-[4-N-(5-aminopentanoic acid)-1-piperidino]-carbonyloxycamptothecin and irinotecan was unaffected by milk thistle (0.332 versus 0.285 versus 0.337; P = 0.53). The maximum plasma concentrations of silybin ranged between 0.0249 and 0.257 micromol/L. Silybin concentrations after intake of milk thistle are too low to significantly affect the function of CYP3A4 and UGT1A1 in vivo, indicating that milk thistle is unlikely to alter the disposition of anticancer drugs metabolized by these enzymes.

  10. Prion-specific Hsp40 function: The role of the auxilin homolog Swa2

    PubMed Central

    Oliver, Emily E.; Troisi, Elizabeth M.; Hines, Justin K.

    2017-01-01

    ABSTRACT Yeast prions are protein-based genetic elements that propagate through cell populations via cytosolic transfer from mother to daughter cell. Molecular chaperone proteins including Hsp70, the Hsp40/J-protein Sis1, and Hsp104 are required for continued prion propagation, however the specific requirements of chaperone proteins differ for various prions. We recently reported that Swa2, the yeast homolog of the mammalian protein auxilin, is specifically required for the propagation of the prion [URE3].1 [URE3] propagation requires both a functional J-domain and the tetratricopeptide repeat (TPR) domain of Swa2, but does not require Swa2 clathrin binding. We concluded that the TPR domain determines the specificity of the genetic interaction between Swa2 and [URE3], and that this domain likely interacts with one or more proteins with a C-terminal EEVD motif. Here we extend that analysis to incorporate additional data that supports this hypothesis. We also present new data eliminating Hsp104 as the relevant Swa2 binding partner and discuss our findings in the context of other recent work involving Hsp90. Based on these findings, we propose a new model for Swa2's involvement in [URE3] propagation in which Swa2 and Hsp90 mediate the formation of a multi-protein complex that increases the number of sites available for Hsp104 disaggregation. PMID:28574745

  11. Precious Metal Distributions in Direct Nickel Matte Smelting with Low-Cu Mattes

    NASA Astrophysics Data System (ADS)

    Piskunen, P.; Avarmaa, K.; O'Brien, H.; Klemettinen, L.; Johto, H.; Taskinen, P.

    2018-02-01

    Base metal (Cu, Fe, and Ni) and trace element (Ag, Au, Co, Pd, and Pt) distributions between low-iron nickel mattes with [Ni]:[Cu] = 4 (w/w) have been studied at 1623 K to 1723 K (1350 °C to 1450 °C). We equilibrated small slag-matte samples with CO-CO2-SO2-Ar atmospheres in pre-selected P_{{{S}2 }} - P_{{{O}2 }} points, maintaining silica saturation by fused silica crucibles. The slags studied contained about 0 to 8.5 wt pct MgO. The matte-slag distribution coefficients L m/s[Me] were obtained from assays by electron probe X-ray microanalysis for the matte and by laser ablation-ICP-mass spectrometry for the slag. The measured L m/s[Me] values were clearly dependent on iron concentration of the matte and on MgO concentration of the slag, with values on the order of 104, 105, and 104 for gold, platinum, and palladium, respectively, in the 5 wt pct iron in matte experiments. The obtained data for silver were scattered, due to volatilization, resulting in depletion of most silver and its escape from matte to gas phase during the 3-hour equilibration period. The matte-to-slag distribution coefficient for silver was estimated to be L m/s[Ag] = 100 to 400. We also measured the distributions of the base metals Cu and Ni in the same conditions as the trace elements.

  12. Evaluation of the attenuating properties of selected Greek clays for toxic inorganic elements in landfill sites.

    PubMed

    Mimides, T; Perraki, T

    2000-05-15

    Heavy metal attenuation properties of selected clay material collected from miscellaneous Greek sites is investigated and tested in the laboratory for their suitability, either as liners in hydrologically unsafe sites or as earth covers for sanitary landfill sites. Eleven potentially hazardous elements (As, Be, Cd, Cr, Cu, Hg, Ni, Pb, Se, V, and Zn) generated by a co-disposal landfill leachate have been considered. Experimental column and static equilibrium methods for the determination of dispersion and adsorption are described. Molecular diffusion dominates the migration phenomena with a velocity range between 1.3 x 10(-5) and 3.5 x 10(-4) cm/s throughout the experiments. A simple way to evaluate dispersion coefficients from breakthrough curves gave values of between 3.90 x 10(-6) and 3.5 x 10(-4) cm2/s, with a mean value of 1.5 x 10(-5). Static adsorption equilibrium studies supported by column runs showed that Freundlich (F = kCn) isotherms express in a better way the assimilative capacities of the tested clays, with k and n values ranging from 0.06 to 1.99 and 0.55 to 1.48 correspondingly. Mathematical models involving non-linear parabolic equations are involved. The experimental data, together with finite difference techniques and some physical clay characteristics, produced trilinear textural diagrams and predictive flow transport convection-dispersion breakthrough curves for a quick estimation of the attenuating properties of clays for heavy metals.

  13. 40 CFR 466.13 - New source performance standards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units—pounds... Nickel 2.46 0.31 1.29 0.16 Zinc 2.09 0.27 0.86 0.11 Aluminum 6.21 0.78 2.54 0.32 Iron 5.74 0.72 2.87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within the range 7...

  14. 40 CFR 466.13 - New source performance standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units—pounds... Nickel 2.46 0.31 1.29 0.16 Zinc 2.09 0.27 0.86 0.11 Aluminum 6.21 0.78 2.54 0.32 Iron 5.74 0.72 2.87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within the range 7...

  15. Transmission Line for 258 GHz Gyrotron DNP Spectrometry

    NASA Astrophysics Data System (ADS)

    Bogdashov, Alexandr A.; Belousov, Vladimir I.; Chirkov, Alexey V.; Denisov, Gregory G.; Korchagin, Vyacheslav V.; Kornishin, Sergey Yu.; Tai, Evgeny M.

    2011-06-01

    We describe the design and test results of the transmission line for liquid-state (LS) and solid-state (SS) DNP spectrometers with the second-harmonic 258.6 GHz gyrotron at the Institute of the Biophysical Chemistry Center of Goethe University (Frankfurt). The 13-meter line includes a mode converter, HE11 waveguides, 4 mitre bends, a variable polarizer-attenuator, directional couplers, a water-flow calorimeter and a mechanical switch. A microwave power of about 15 W was obtained in the pure HE11 mode at the spectrometer inputs.

  16. First isolate of KPC-2-producing Klebsiella pneumonaie sequence type 23 from the Americas.

    PubMed

    Cejas, Daniela; Fernández Canigia, Liliana; Rincón Cruz, Giovanna; Elena, Alan X; Maldonado, Ivana; Gutkind, Gabriel O; Radice, Marcela A

    2014-09-01

    KPC-2-producing Klebsiella pneumoniae isolates mainly correspond to clonal complex 258 (CC258); however, we describe KPC-2-producing K. pneumoniae isolates belonging to invasive sequence type 23 (ST23). KPC-2 has scarcely been reported to occur in ST23, and this report describes the first isolation of this pathogen in the Americas. Acquisition of resistant markers in virulent clones could mark an evolutionary step toward the establishment of these clones as major nosocomial pathogens. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  17. To amend section 2710 of title 18, United States Code, to clarify that a video tape service provider may obtain a consumer's informed, written consent on an ongoing basis and that consent may be obtained through the Internet.

    THOMAS, 112th Congress

    Rep. Goodlatte, Bob [R-VA-6

    2011-07-08

    Senate - 12/20/2012 By Senator Leahy from Committee on the Judiciary filed written report. Report No. 112-258. Additional views filed. (All Actions) Notes: For further action, see H.R.6671, which became Public Law 112-258 on 1/10/2013. Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  18. An exploratory study of the potential of LIBS for visualizing gunshot residue patterns.

    PubMed

    López-López, María; Alvarez-Llamas, César; Pisonero, Jorge; García-Ruiz, Carmen; Bordel, Nerea

    2017-04-01

    The study of gunshot residue (GSR) patterns can assist in the reconstruction of shooting incidences. Currently, there is a real need of methods capable of furnishing simultaneous elemental analysis with higher specificity for the GSR pattern visualization. Laser-Induced Breakdown Spectroscopy (LIBS) provides a multi-elemental analysis of the sample, requiring very small amounts of material and no sample preparation. Due to these advantages, this study aims at exploring the potential of LIBS imaging for the visualization of GSR patterns. After the spectral characterization of individual GSR particles, the distribution of Pb, Sb and Ba over clothing targets, shot from different distances, were measured in laser raster mode. In particular, an array of spots evenly spaced at 800μm, using a stage displacement velocity of 4mm/s and a laser frequency of 5Hz was employed (e.g. an area of 130×165mm 2 was measured in less than 3h). A LIBS set-up based on the simultaneous use of two spectrographs with iCCD cameras and a motorized stage was used. This set-up allows obtaining information from two different wavelength regions (258-289 and 446-463nm) from the same laser induced plasma, enabling the simultaneous detection of the three characteristic elements (Pb, Sb, and Ba) of GSR particles from conventional ammunitions. The ability to visualize the 2D distribution GSR pattern by LIBS may have an important application in the forensic field, especially for the ballistics area. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Aspherical-atom modeling of coordination compounds by single-crystal X-ray diffraction allows the correct metal atom to be identified.

    PubMed

    Dittrich, Birger; Wandtke, Claudia M; Meents, Alke; Pröpper, Kevin; Mondal, Kartik Chandra; Samuel, Prinson P; Amin Sk, Nurul; Singh, Amit Pratap; Roesky, Herbert W; Sidhu, Navdeep

    2015-02-02

    Single-crystal X-ray diffraction (XRD) is often considered the gold standard in analytical chemistry, as it allows element identification as well as determination of atom connectivity and the solid-state structure of completely unknown samples. Element assignment is based on the number of electrons of an atom, so that a distinction of neighboring heavier elements in the periodic table by XRD is often difficult. A computationally efficient procedure for aspherical-atom least-squares refinement of conventional diffraction data of organometallic compounds is proposed. The iterative procedure is conceptually similar to Hirshfeld-atom refinement (Acta Crystallogr. Sect. A- 2008, 64, 383-393; IUCrJ. 2014, 1,61-79), but it relies on tabulated invariom scattering factors (Acta Crystallogr. Sect. B- 2013, 69, 91-104) and the Hansen/Coppens multipole model; disordered structures can be handled as well. Five linear-coordinate 3d metal complexes, for which the wrong element is found if standard independent-atom model scattering factors are relied upon, are studied, and it is shown that only aspherical-atom scattering factors allow a reliable assignment. The influence of anomalous dispersion in identifying the correct element is investigated and discussed. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Co/Ni Ratio Between Is Approximately 0.35 - 8.0 GeV/nucleon from the TIGER-2001 Flight

    NASA Technical Reports Server (NTRS)

    deNolfo, G. A.; Barbier, L. M.; Binns, W. R.; Cummings, J. R.; Geier, S.; Israel, M. N.; Link, J. T.; Mewaldt, R. A.; Mitchell, J. W.; Rauch, B. F.; hide

    2007-01-01

    The Trans-Iron Galactic Element Recorder (TIGER) was launched in December 2001 and 2003 from McMurdo, Antarctica and was designed to observe elements ranging from 14 < Z < 40 over an extended energy range. Observations of radioactive isotopes produced during explosive nucleosynthesis such as Ni-59 that decay only through electron capture provide important constraints on the delay between nucleosynthesis and the acceleration of galactic cosmic rays (GCRs). The isotopes of Co and Ni at low energies, in particular, the observations of the Ni-59 and Co-59 from the Cosmic Ray Isotope Spectrometer (CRIS) on the Advanced Composition Explorer, indicate a significant time delay (greater than 7.6 x 10^4 yr) between GCR nucleosynthesis and acceleration. While TIGER is not able to resolve isotopes, observations of the elemental abundances of Co and Ni at high energies further constrain models for the acceleration and propagation of GCRs. The 2001 & 2003 flights of TIGER lasted a total of approx. 50 days and collected sufficient statistics to study the Co/Ni elemental ratio over a wide range in energies. We present the elemental ratio of Co/Ni in galactic cosmic rays between approx. 0.8- 5.0 GeV/nucleon and compare these results with previous measurements and models for cosmic-ray propagation.

  1. Partition Coefficients at High Pressure and Temperature

    NASA Astrophysics Data System (ADS)

    Righter, K.; Drake, M. J.

    2003-12-01

    Differentiation of terrestrial planets includes separation of a metallic core and possible later fractionation of mineral phases within either a solid or molten mantle (Figure 1). Lithophile and siderophile elements can be used to understand these two different physical processes, and ascertain whether they operated in the early Earth. The distribution of elements in planets can be understood by measuring the partition coefficient, D (ratio of concentrations of an element in different phases (minerals, metals, or melts)). (14K)Figure 1. Schematic cross-section through the Earth, showing: (a) an early magma ocean stage and (b) a later cool and differentiated stage. The siderophile elements (iron-loving) encompass over 30 elements and are defined as those elements for which D(metal/silicate)>1, and are useful for deciphering the details of core formation. This group of elements is commonly broken up into several subclasses, including the slightly siderophile elements (1104). Because these three groups encompass a wide range of partition coefficient values, they can be very useful in trying to determine the conditions under which metal may have equilibrated with the mantle (or a magma ocean). Because metal and silicate may equilibrate by several different mechanisms, such as at the base of a deep magma ocean, or as metal droplets descend through a molten mantle, partition coefficients can potentially shed light on which mechanism may be most important, thus linking the physics and chemistry of core formation. In this chapter, we summarize metal/silicate partitioning of siderophile elements and show how they may be used to understand planetary core formation.Once a planet is differentiated into core and mantle, a mantle will cool during convection, and can start in either a molten or solid state, depending upon the initial thermal conditions. If hot enough, minerals will crystallize from a molten mantle, and become entrained in the convecting melt, or eventually settle out at the bottom. The entrainment and settling process has been studied in detail (e.g., Tonks and Melosh, 1990), and is a potential mechanism for differentiation between the deep and shallow parts of Earth's mantle. The lithophile elements, those elements that have D(metal/silicate) <1, fall into many different subclasses and all hold information about the deep mineral structure of the mantle. Rare-earth elements (REEs) have proven to be useful: europium anomalies have helped elucidate the role of plagioclase in lunar crust formation (e.g., Schnetzler and Philpotts, 1971; Weill et al., 1974), and LREE/HREE depletion and enrichment are indicators of partial melting in the presence of garnet in the mantle. High-field-strength elements (HFSEs) - niobium, zirconium, tantalum, and hafnium - are all refractory and hence more resilient to fractionation processes such as volatility or condensation. They also have an affinity for ilmenite and rutile, and can explain differences between lunar and martian samples as well as features of Earth's continental crust ( Taylor and McLennan, 1985). Alkaline-earth and alkaline elements include rubidium, strontium, barium, potassium, caesium, and calcium, some of which are involved in radioactive decay couples, e.g., Rb-Sr and K-Ar. The latter is important in understanding the contribution of radioactive decay to planetary heat production, and potential deep sources of radiogenic argon (see Chapter 2.06). Rubidium and potassium are further useful as tracers of hydrous phases such as mica and amphibole. Possible fractionation of any of these elements from chondritic abundances (see Chapter 2.01) can be assessed with the knowledge of partition coefficients. In this chapter we summarize our understanding of mineral/melt fractionation of minor and trace elements at high pressures and temperatures and discuss the implications for mantle differentiation.

  2. Geochemistry of Groundwater

    NASA Astrophysics Data System (ADS)

    Chapelle, F. H.

    2003-12-01

    Differentiation of terrestrial planets includes separation of a metallic core and possible later fractionation of mineral phases within either a solid or molten mantle (Figure 1). Lithophile and siderophile elements can be used to understand these two different physical processes, and ascertain whether they operated in the early Earth. The distribution of elements in planets can be understood by measuring the partition coefficient, D (ratio of concentrations of an element in different phases (minerals, metals, or melts)). (14K)Figure 1. Schematic cross-section through the Earth, showing: (a) an early magma ocean stage and (b) a later cool and differentiated stage. The siderophile elements (iron-loving) encompass over 30 elements and are defined as those elements for which D(metal/silicate)>1, and are useful for deciphering the details of core formation. This group of elements is commonly broken up into several subclasses, including the slightly siderophile elements (1104). Because these three groups encompass a wide range of partition coefficient values, they can be very useful in trying to determine the conditions under which metal may have equilibrated with the mantle (or a magma ocean). Because metal and silicate may equilibrate by several different mechanisms, such as at the base of a deep magma ocean, or as metal droplets descend through a molten mantle, partition coefficients can potentially shed light on which mechanism may be most important, thus linking the physics and chemistry of core formation. In this chapter, we summarize metal/silicate partitioning of siderophile elements and show how they may be used to understand planetary core formation.Once a planet is differentiated into core and mantle, a mantle will cool during convection, and can start in either a molten or solid state, depending upon the initial thermal conditions. If hot enough, minerals will crystallize from a molten mantle, and become entrained in the convecting melt, or eventually settle out at the bottom. The entrainment and settling process has been studied in detail (e.g., Tonks and Melosh, 1990), and is a potential mechanism for differentiation between the deep and shallow parts of Earth's mantle. The lithophile elements, those elements that have D(metal/silicate) <1, fall into many different subclasses and all hold information about the deep mineral structure of the mantle. Rare-earth elements (REEs) have proven to be useful: europium anomalies have helped elucidate the role of plagioclase in lunar crust formation (e.g., Schnetzler and Philpotts, 1971; Weill et al., 1974), and LREE/HREE depletion and enrichment are indicators of partial melting in the presence of garnet in the mantle. High-field-strength elements (HFSEs) - niobium, zirconium, tantalum, and hafnium - are all refractory and hence more resilient to fractionation processes such as volatility or condensation. They also have an affinity for ilmenite and rutile, and can explain differences between lunar and martian samples as well as features of Earth's continental crust ( Taylor and McLennan, 1985). Alkaline-earth and alkaline elements include rubidium, strontium, barium, potassium, caesium, and calcium, some of which are involved in radioactive decay couples, e.g., Rb-Sr and K-Ar. The latter is important in understanding the contribution of radioactive decay to planetary heat production, and potential deep sources of radiogenic argon (see Chapter 2.06). Rubidium and potassium are further useful as tracers of hydrous phases such as mica and amphibole. Possible fractionation of any of these elements from chondritic abundances (see Chapter 2.01) can be assessed with the knowledge of partition coefficients. In this chapter we summarize our understanding of mineral/melt fractionation of minor and trace elements at high pressures and temperatures and discuss the implications for mantle differentiation.

  3. Nucleocapsid protein-dependent assembly of the RNA packaging signal of Middle East respiratory syndrome coronavirus.

    PubMed

    Hsin, Wei-Chen; Chang, Chan-Hua; Chang, Chi-You; Peng, Wei-Hao; Chien, Chung-Liang; Chang, Ming-Fu; Chang, Shin C

    2018-05-24

    Middle East respiratory syndrome coronavirus (MERS-CoV) consists of a positive-sense, single-stranded RNA genome and four structural proteins: the spike, envelope, membrane, and nucleocapsid protein. The assembly of the viral genome into virus particles involves viral structural proteins and is believed to be mediated through recognition of specific sequences and RNA structures of the viral genome. A culture system for the production of MERS coronavirus-like particles (MERS VLPs) was determined and established by electron microscopy and the detection of coexpressed viral structural proteins. Using the VLP system, a 258-nucleotide RNA fragment, which spans nucleotides 19,712 to 19,969 of the MERS-CoV genome (designated PS258(19712-19969) ME ), was identified to function as a packaging signal. Assembly of the RNA packaging signal into MERS VLPs is dependent on the viral nucleocapsid protein. In addition, a 45-nucleotide stable stem-loop substructure of the PS258(19712-19969) ME interacted with both the N-terminal domain and the C-terminal domain of the viral nucleocapsid protein. Furthermore, a functional SARS-CoV RNA packaging signal failed to assemble into the MERS VLPs, which indicated virus-specific assembly of the RNA genome. A MERS-oV RNA packaging signal was identified by the detection of GFP expression following an incubation of MERS VLPs carrying the heterologous mRNA GFP-PS258(19712-19969) ME with virus permissive Huh7 cells. The MERS VLP system could help us in understanding virus infection and morphogenesis.

  4. Infiltration behaviour of elemental mercury DNAPL in fully and partially water saturated porous media.

    PubMed

    D'Aniello, Andrea; Hartog, Niels; Sweijen, Thomas; Pianese, Domenico

    2018-02-01

    Mercury is a contaminant of global concern due to its harmful effects on human health and for the detrimental consequences of its release in the environment. Sources of liquid elemental mercury are usually anthropogenic, such as chlor-alkali plants. To date insight into the infiltration behaviour of liquid elemental mercury in the subsurface is lacking, although this is critical for assessing both characterization and remediation approaches for mercury DNAPL contaminated sites. Therefore, in this study the infiltration behaviour of elemental mercury in fully and partially water saturated systems was investigated using column experiments. The properties affecting the constitutive relations governing the infiltration behaviour of liquid Hg 0 , and PCE for comparison, were determined using P c (S) experiments with different granular porous media (glass beads and sands) for different two- and three-phase configurations. Results showed that, in water saturated porous media, elemental mercury, as PCE, acted as a non-wetting fluid. The required entry head for elemental mercury was higher (from about 5 to 7 times). However, due to the almost tenfold higher density of mercury, the required NAPL entry heads of 6.19cm and 12.51cm for mercury to infiltrate were 37.5% to 20.7% lower than for PCE for the same porous media. Although Leverett scaling was able to reproduce the natural tendency of Hg 0 to be more prone than PCE to infiltrate in water saturated porous media, it considerably underestimated Hg 0 infiltration capacity in comparison with the experimental results. In the partially water saturated system, in contrast with PCE, elemental mercury also acted as a nonwetting fluid, therefore having to overcome an entry head to infiltrate. The required Hg 0 entry heads (10.45 and 15.74cm) were considerably higher (68.9% and 25.8%) than for the water saturated porous systems. Furthermore, in the partially water saturated systems, experiments showed that elemental mercury displaced both air and water, depending on the initial water distribution within the pores. This indicates that the conventional wettability hierarchy, in which the NAPL has an intermediate wetting state between the air and the water phases, is not valid for liquid elemental mercury. Therefore, for future modelling of elemental mercury DNAPL infiltration behaviour in variably water saturated porous media, a different formulation of the governing constitutive relations will be required. Copyright © 2018 Elsevier B.V. All rights reserved.

  5. Health risk assessment of trace elements via dietary intake of 'non-piscine protein source' foodstuffs (meat, milk and egg) in Bangladesh.

    PubMed

    Shaheen, Nazma; Ahmed, Md Kawser; Islam, Md Saiful; Habibullah-Al-Mamun, Md; Tukun, Avonti Basak; Islam, Saiful; M A Rahim, Abu Torab

    2016-04-01

    Concentrations of six trace elements [chromium (Cr), nickel (Ni), copper (Cu), cadmium (Cd), lead (Pb) and arsenic (As)] were assessed in 'non-piscine protein source' foodstuffs (meat, milk and eggs) to evaluate contamination level and human health risks in Bangladesh. The range of Cr, Ni, Cu, Cd, Pb and As in the investigated foodstuffs was 1.24-2.17, 1.29-2.56, 0.92-2.31, 0.12-0.44, 0.15-0.48 and 0.14-0.57 mg kg fresh weight(-1), respectively. The estimated mean levels of most of the elements were higher than the maximum allowable concentration (MAC) for dietary foods. The estimated daily intakes (EDIs) of Cr and Cd were higher than the maximum tolerable daily intake (MTDI) for children, indicating that they are more susceptible to toxic elements through food consumption. The target hazard quotients (THQs) and target carcinogenic risk (TCR) of As (THQ > 1 and TCR > 10(-4)) for both the adults and children suggest that the consumers of non-piscine foodstuffs (especially cow milk and chicken meat) are exposed chronically to metal pollution with carcinogenic and non-carcinogenic health consequences.

  6. The [KIL-d] element specifically regulates viral gene expression in yeast.

    PubMed Central

    Tallóczy, Z; Mazar, R; Georgopoulos, D E; Ramos, F; Leibowitz, M J

    2000-01-01

    The cytoplasmically inherited [KIL-d] element epigenetically regulates killer virus gene expression in Saccharomyces cerevisiae. [KIL-d] results in variegated defects in expression of the M double-stranded RNA viral segment in haploid cells that are "healed" in diploids. We report that the [KIL-d] element is spontaneously lost with a frequency of 10(-4)-10(-5) and reappears with variegated phenotypic expression with a frequency of > or =10(-3). This high rate of loss and higher rate of reappearance is unlike any known nucleic acid replicon but resembles the behavior of yeast prions. However, [KIL-d] is distinct from the known yeast prions in its relative guanidinium hydrochloride incurability and independence of Hsp104 protein for its maintenance. Despite its transmissibility by successive cytoplasmic transfers, multiple cytoplasmic nucleic acids have been proven not to carry the [KIL-d] trait. [KIL-d] epigenetically regulates the expression of the M double-stranded RNA satellite virus genome, but fails to alter the expression of M cDNA. This specificity remained even after a cycle of mating and meiosis. Due to its unique genetic properties and viral RNA specificity, [KIL-d] represents a new type of genetic element that interacts with a viral RNA genome. PMID:10835384

  7. Useful ion yields for Cameca IMS 3f and 6f SIMS: Limits on quantitative analysis

    USGS Publications Warehouse

    Hervig, R.L.; Mazdab, F.K.; Williams, Pat; Guan, Y.; Huss, G.R.; Leshin, L.A.

    2006-01-01

    The useful yields (ions detected/atom sputtered) of major and trace elements in NIST 610 glass were measured by secondary ion mass spectrometry (SIMS) using Cameca IMS 3f and 6f instruments. Useful yields of positive ions at maximum transmission range from 10-4 to 0.2 and are negatively correlated with ionization potential. We quantified the decrease in useful yields when applying energy filtering or high mass resolution techniques to remove molecular interferences. The useful yields of selected negative ions (O, S, Au) in magnetite and pyrite were also determined. These data allow the analyst to determine if a particular analysis (trace element contents or isotopic ratio) can be achieved, given the amount of sample available and the conditions of the analysis. ?? 2005 Elsevier B.V. All rights reserved.

  8. Effects of a Behavioral and Exercise Program on Depression and Quality of Life in Community-Dwelling Older Adults: A Controlled, Quasi-Experimental Study.

    PubMed

    Azizan, Azilyana; Justine, Maria

    2016-02-01

    Sedentary behavior and low participation in exercise among older adults can lead to depression and low quality of life (QOL). The current study investigated the effects of behavioral and exercise programs on depression severity and QOL among Malaysian community-dwelling older adults. A controlled, quasi-experimental, pre-posttest design was used. A total of 63 participants were divided into three groups: (a) exercise and behavior group (EBG), (b) exercise only group (EG), and (c) control group (CG). Results showed a significant difference in depression among groups (F(2,58) = 33.49, p < 0.01, η(2) = 0.54; mean, EBG < EG < CG) and in physical (F(2,58) = 5.33, p < 0.01, η(2)= 0.16; mean, EBG > EG > CG) and mental (F(2,58) = 4.08, p < 0.01, η(2) = 0.12; mean, EBG > CG > EG) scores of QOL. A combination of behavioral and exercise programs has superior effects on depression and QOL of older adults. [Journal of Gerontological Nursing, 42(2), 45-54.]. Copyright 2016, SLACK Incorporated.

  9. The C-terminal domain of connexin43 modulates cartilage structure via chondrocyte phenotypic changes

    PubMed Central

    Gago-Fuentes, Raquel; Bechberger, John F.; Varela-Eirin, Marta; Varela-Vazquez, Adrian; Acea, Benigno; Fonseca, Eduardo

    2016-01-01

    Chondrocytes in cartilage and bone cells population express connexin43 (Cx43) and gap junction intercellular communication (GJIC) is essential to synchronize cells for coordinated electrical, mechanical, metabolic and chemical communication in both tissues. Reduced Cx43 connectivity decreases chondrocyte differentiation and defective Cx43 causes skeletal defects. The carboxy terminal domain (CTD) of Cx43 is located in the cytoplasmic side and is key for protein functions. Here we demonstrated that chondrocytes from the CTD-deficient mice, K258stop/Cx43KO and K258stop/K258stop, have reduced GJIC, increased rates of proliferation and reduced expression of collagen type II and proteoglycans. We observed that CTD-truncated mice were significantly smaller in size. Together these results demonstrated that the deletion of the CTD negatively impacts cartilage structure and normal chondrocyte phenotype. These findings suggest that the proteolytic cleavage of the CTD under pathological conditions, such as under the activation of metalloproteinases during tissue injury or inflammation, may account for the deleterious effects of Cx43 in cartilage and bone disorders such as osteoarthritis. PMID:27682878

  10. Origin of electrically heterogeneous microstructure in CuO from scanning tunneling spectroscopy study

    NASA Astrophysics Data System (ADS)

    Sarkar, Sudipta; Jana, Pradip Kumar; Chaudhuri, B. K.

    2008-04-01

    We report electronic structure of the grains and grain boundaries (GBs) of the high permittivity (κ˜104) ceramic CuO from scanning tunneling spectroscopy (STS) studies. The p-type semiconducting character of the CuO grains and insulating behavior of the corresponding GBs, observed from STS studies, have been explained. This type of electrically inhomogeneous microstructure leads to the formation of barrier layer capacitance elements in CuO and, hence, provides an explanation of the colossal-κ response exhibited by CuO.

  11. Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with Various Plasmids

    PubMed Central

    Serror, Pascale; Sasaki, Takashi; Ehrlich, S. Dusko; Maguin, Emmanuelle

    2002-01-01

    We describe, for the first time, a detailed electroporation procedure for Lactobacillus delbrueckii. Three L. delbrueckii strains were successfully transformed. Under optimal conditions, the transformation efficiency was 104 transformants per μg of DNA. Using this procedure, we identified several plasmids able to replicate in L. delbrueckii and integrated an integrative vector based on phage integrative elements into the L. delbrueckii subsp. bulgaricus chromosome. These vectors provide a good basis for developing molecular tools for L. delbrueckii and open the field of genetic studies in L. delbrueckii. PMID:11772607

  12. Further Insight into the Reaction FeO+ + H2 Yields Fe+ + H2O: Temperature Dependent Kinetics, Isotope Effects, and Statistical Modeling (Postprint)

    DTIC Science & Technology

    2014-07-31

    a laminar flow tube via a Venturi inlet, where ∼104 to 105 collisions with a He buffer gas act to thermalize the ions and carry them downstream...ISOTOPE EFFECTS , AND STATISTICAL MODELING (POSTPRINT) Shaun G. Ard, et al. 31 July 2014 Journal Article AIR FORCE RESEARCH LABORATORY Space Vehicles...Kinetics, Isotope Effects , and Statistical Modeling (Postprint) 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 61102F 6

  13. A novel methanol sensor based on gas-penetration through a porous polypyrrole-coated polyacrylonitrile nanofiber mat.

    PubMed

    Jun, Tae-Sun; Ho, Thi Anh; Rashid, Muhammad; Kim, Yong Shin

    2013-09-01

    In this work, we propose a novel chemoresistive gas sensor operated under a vertical analyte flow passing through a permeable sensing membrane. Such a configuration is different from the use of a planar sensor implemented under a conventional horizontal flow. A highly porous core-shell polyacrylonitrile-polypyrrole (PAN@PPy) nanofiber mat was prepared as the sensing element via electrospinning and two-step vapor-phase polymerization (VPP). Various analysis methods such as SEM, TEM, FT-IR and XPS measurements were employed in order to characterize structural features of the porous sensing mat. These analyses confirmed that very thin (ca. 10 nm) conductive PPy sheath layers were deposited by VPP on electrospun PAN nanofibers with an average diameter of 258 nm. Preliminary results revealed that the gas penetration-type PAN@PPy sensor had a higher sensor response and shorter detection and recovery times upon exposure to methanol analyte when compared with a conventional horizontal flow sensor due to efficient and fast analyte transfer into the sensing layer.

  14. A determination of the CKM-matrix element ratio | V ts |/| V cb | from the rare B-decays B→ K *+γ and B→ X s +γ

    NASA Astrophysics Data System (ADS)

    Ali, A.; Greub, C.

    1993-09-01

    Implications of the recent CLEO observation [1] of the rare decay mode B→ K *+γ having a combined branching ratio BR( B→ K *+γ)=(4.5±1.5±0.9)×10-5 and an improved upper limit on the inclusive branching ratio BR( B→ X s +γ)<5.4×10-4 (95% C.L.) [2] are discussed in the context of the Standard Model (SM). Using the unitarity of the CKM-matrix and taking into account QCD radiative corrections in the decay rate and the inclusive photon energy spectrum we obtain an improved upper limit on the inclusive branching ratio BR( B→ X s +γ)<4.8×10-4 (95% C.L.). This can be used to constrain possible non-SM contributions to the inclusive branching ratio, giving BR( B→ X s +γ) (non-SM)<3.0×10-4 for m t >-108 GeV. Within the SM, we show that the resulting experimental upper limit can be interpreted as a corresponding limit on the CKM-matrix element ratio yielding | V ts |/| V cb |<1.67, with the top quark mass assumed to weigh less than 200 GeV. We calculate the relative exclusive to inclusive branching ratio R( K */ X s )≡Γ( B→ K *+γ)/Γ( B→ X s +γ), based on the inclusive hadronic invariant mass distribution in B→ X s +γ. Estimating the K *-contribution from this distribution in the threshold region ( m K + m π)≤ m X s ≤0.97 GeV and using experimental measurements from the semileptonic D-decays D→ K+π+ℓ v ℓ in the same mass interval, we obtain R( K */ X s )=0.13±0.03. This enables us to put a lower bound on the ratio | V ts |/| V cb | from the 95% C.L. lower limit on the branching ratio BR( B→ K *+γ)>1.6×10-5 [1,2]. Combining the exclusive and inclusive decay rates, we determine 0.50≤| V ts |/| V cb |≤1.67 (at 95% C.L.).

  15. Calcium Isotopic Systematics of Peridotite Xenoliths from eastern North China Craton: Implications for Melt-rock Interaction

    NASA Astrophysics Data System (ADS)

    Zhang, Z.; Zhao, X.; Huang, S.; Xiao, Y.; Li, X.

    2017-12-01

    In order to better constrain and understand the Ca isotopic variations in the mantle, we report high-precision Ca isotopic data of orthopyroxene (Opx) and clinopyroxene (Cpx) for a set of peridotitic xenoliths from ChangLe, eastern North China craton (NCC). These xenoliths range from lherzolites, Cpx-rich lherzolites to wehrlites, and are variably metasomatised. Lherzolites (Fo≈91) are fertile in mineral composition, and have spoon-shaped to slightly LREE-enriched rare earth element (REE) patterns. They may represent fragments of newly accreted lithospheric mantle that makes up parts of the Late Mesozoic-Cenozoic lithosphere beneath the eastern NCC. Cpx-rich lherzolites and wehrlites formed by reaction of refractory residual peridotites with evolved, Fe-rich silicate melts at high melt/rock ratios, as evidenced by partial to complete replacement of Opx with Cpx, relatively lower Fo contents of Ol (<88) than that from the lherzolites and convex-upward trace element patterns. Our results show that there are large δ44/40Ca variations (1‰) in these peridotitic xenoliths. Lherzolites have δ44/40Ca similar to typical upper mantle value(1.05 ± 0.04). Specifically, lherzolites orthopyroxenes have δ44/40Ca ranging from 1.04 to 1.79, and lherzolites clinopyroxenes have δ44/40Ca from 0.80 to 1.04. In contrast, δ44/40Ca in Cpx-rich lherzolites and wehrlites tend to have lower values reltaive to those of lherzolites. Their clinopyroxenes have δ44/40Ca ranging from 0.42 to 0.92, and their orthopyroxenes have δ44/40Ca ranging from 0.80 to 1.04. Collectively, we identify a positive correlation between clinopyroxene δ44/40Ca and Mg#. Model calculations show that kinetic isotopic fractionation caused by diffusion, probably during mantle melt-rock interaction, is responsible for the positive correlation between clinopyroxene δ44/40Ca and Mg# in these NCC peridotites. Our study shows that melt-rock interaction plays an important role in producing Ca isotopic heterogeneity of the subcontinental mantle.

  16. Compensation effect of bacterium containing biofertilizer on the growth of Cucumis sativus L. under Al-stress conditions.

    PubMed

    Tóth, Brigitta; Lévai, L; Kovács, B; Varga, Mária Borbélyné; Veres, Szilvia

    2013-03-01

    Biofertilizers are used to improve soil fertility and plant production in sustainable agriculture. However, their applicability depends on several environmental parameters. The aim of our study was to evaluate the effect of free-living bacteria containing fertilizer on the growth of cucumber (Cucumis sativus L. cvs. Delicates) under aluminium (Al) stress. Different responses to Al stress of cucumber growth parameters were examined in terms of root elongation and physiological traits, such as Spad index (relative chlorophyll value), biomass accumulation of root and shoot, Al uptake and selected element contents (Fe, Mn, Zn, Mg) of leaves and root. The applied bacteria containing biofertilizer contains Azotobacter chroococcum and Bacillus megaterium. The dry weights of cucumber shoots and roots decreased in line with the increasing Al concentration. Due to different Al treatments (10-3 M, 10-4 M) higher Al concentration was observed in the leaves, while the amounts of other elements (Fe, Mn, Zn, Mg) decreased. This high Al content of the leaves decreased below the control value when biofertilizer was applied. In the case of the roots the additional biofertilizer treatments compensated the effect of Al. The relative chlorophyll content was reduced during Al-stress in older plants and the biofertilizer moderated this effect. The root/shoot ratio was decreased in all the Al-treatments in comparison to the control. The living bacteria containing fertilizer also had a modifying effect. The root/shoot ratio increased at the 10-4 M Al2(SO4)2 + biofertilizer and 10-4 M Al(NO3)3 + biofertilizer treatments compared to the control and Al-treatments. According to our results the biofertilizer is an alternative nutrient supply for replacing chemical fertilizers because it enhances dry matter production. Biofertilizer usage is also offered under Al polluted environmental conditions. Although, the nutrient solution is a clean system where we can examine the main processes without other effects of natural soils. The soil can modify the results, e.g. the soil-born microorganisms affect nutrient availability, and also can modify the harmful effects of different heavy metals. The understanding of basic processes will help us to know more about the soil behaviour.

  17. 40 CFR 466.33 - New source performance standards.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 6.13 0.79 Oil and grease 97.24 12.60 97.24 12.60 TSS 145.86 18.91 116.69 15.12 pH (1) (1) (1) (1... 0.03 0.18 0.20 Nickel 1.10 0.14 0.74 0.10 Zinc 2.03 0.27 0.84 0.11 Aluminum 6.03 0.78 2.47 0.32 Iron 2.45 0.32 1.26 0.16 Oil and grease 19.92 2.58 19.92 2.58 TSS 29.88 3.87 23.90 3.10 pH (1) (1) (1) (1...

  18. 40 CFR 466.33 - New source performance standards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 6.13 0.79 Oil and grease 97.24 12.60 97.24 12.60 TSS 145.86 18.91 116.69 15.12 pH (1) (1) (1) (1... 0.03 0.18 0.20 Nickel 1.10 0.14 0.74 0.10 Zinc 2.03 0.27 0.84 0.11 Aluminum 6.03 0.78 2.47 0.32 Iron 2.45 0.32 1.26 0.16 Oil and grease 19.92 2.58 19.92 2.58 TSS 29.88 3.87 23.90 3.10 pH (1) (1) (1) (1...

  19. 40 CFR 466.33 - New source performance standards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 6.13 0.79 Oil and grease 97.24 12.60 97.24 12.60 TSS 145.86 18.91 116.69 15.12 pH (1) (1) (1) (1... 0.03 0.18 0.20 Nickel 1.10 0.14 0.74 0.10 Zinc 2.03 0.27 0.84 0.11 Aluminum 6.03 0.78 2.47 0.32 Iron 2.45 0.32 1.26 0.16 Oil and grease 19.92 2.58 19.92 2.58 TSS 29.88 3.87 23.90 3.10 pH (1) (1) (1) (1...

  20. Children and Youth Camp Safety Act, 1978. Hearing Before the Subcommittee on Child and Human Development of the Committee on Human Resources, United States Senate, 95th Congress, 2nd Session on S. 258--To Provide for the Development and Implementation of Programs for Children and Youth Camp Safety (March 21, 1978).

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Committee on Human Resources.

    The product of some 10 years of work directed toward federal legislation addressing and defining youth camp safety, the Youth Camp Safety Act (S. 258), as presented in these hearings, calls for the federal government to assume a role in the development of state health and safety standards for children attending youth camps in any state in the…

  1. Apparatus for Study of Ion-Thruster Propellant Ionization

    DTIC Science & Technology

    2006-12-01

    extracted . Releasing these electrons can be done in several ways. Thermionic photo emission and field emissions are valid options. All the...50.9 80.7 106 159 208 503]; %% pressure in millitorr P2=P2/1000; %% pressure in torr Vb2 =[296 276 248 258 258 274 324]; %% voltage in volts pd2=d2...262 276 348]; %% voltage in volts pd3=d3*P3; figure(1) plot(pd1,Vb1,’bo-’,’linewidth’,2) hold on plot(pd2, Vb2 ,’rs:’,’linewidth’,2) hold on 42

  2. New multiplex PCR methods for rapid screening of genetically modified organisms in foods

    PubMed Central

    Datukishvili, Nelly; Kutateladze, Tamara; Gabriadze, Inga; Bitskinashvili, Kakha; Vishnepolsky, Boris

    2015-01-01

    We present novel multiplex PCR methods for rapid and reliable screening of genetically modified organisms (GMOs). New designed PCR primers targeting four frequently used GMO specific sequences permitted identification of new DNA markers, in particular 141 bp fragment of cauliflower mosaic virus (CaMV) 35S promoter, 224 bp fragment of Agrobacterium tumefaciens nopaline synthase (NOS) terminator, 256 bp fragment of 5-enolppyruvylshikimate-phosphate synthase (epsps) gene and 258 bp fragment of Cry1Ab delta-endotoxin (cry1Ab) gene for GMO screening. The certified reference materials containing Roundup Ready soybean (RRS) and maize MON 810 were applied for the development and optimization of uniplex and multiplex PCR systems. Evaluation of amplification products by agarose gel electrophoresis using negative and positive controls confirmed high specificity and sensitivity at 0.1% GMO for both RRS and MON 810. The fourplex PCR was developed and optimized that allows simultaneous detection of three common transgenic elements, such as: CaMV 35S promoter, NOS terminator, epsps gene together with soybean-specific lectin gene. The triplex PCR developed enables simultaneous identification of transgenic elements, such as: 35S promoter and cry1Ab gene together with maize zein gene. The analysis of different processed foods demonstrated that multiplex PCR methods developed in this study are useful for accurate and fast screening of GM food products. PMID:26257724

  3. New multiplex PCR methods for rapid screening of genetically modified organisms in foods.

    PubMed

    Datukishvili, Nelly; Kutateladze, Tamara; Gabriadze, Inga; Bitskinashvili, Kakha; Vishnepolsky, Boris

    2015-01-01

    We present novel multiplex PCR methods for rapid and reliable screening of genetically modified organisms (GMOs). New designed PCR primers targeting four frequently used GMO specific sequences permitted identification of new DNA markers, in particular 141 bp fragment of cauliflower mosaic virus (CaMV) 35S promoter, 224 bp fragment of Agrobacterium tumefaciens nopaline synthase (NOS) terminator, 256 bp fragment of 5-enolppyruvylshikimate-phosphate synthase (epsps) gene and 258 bp fragment of Cry1Ab delta-endotoxin (cry1Ab) gene for GMO screening. The certified reference materials containing Roundup Ready soybean (RRS) and maize MON 810 were applied for the development and optimization of uniplex and multiplex PCR systems. Evaluation of amplification products by agarose gel electrophoresis using negative and positive controls confirmed high specificity and sensitivity at 0.1% GMO for both RRS and MON 810. The fourplex PCR was developed and optimized that allows simultaneous detection of three common transgenic elements, such as: CaMV 35S promoter, NOS terminator, epsps gene together with soybean-specific lectin gene. The triplex PCR developed enables simultaneous identification of transgenic elements, such as: 35S promoter and cry1Ab gene together with maize zein gene. The analysis of different processed foods demonstrated that multiplex PCR methods developed in this study are useful for accurate and fast screening of GM food products.

  4. Early Nephrology Referral 6 Months Before Dialysis Initiation Can Reduce Early Death But Does Not Improve Long-Term Cardiovascular Outcome on Dialysis.

    PubMed

    Hayashi, Terumasa; Kimura, Tomonori; Yasuda, Keiko; Sasaki, Koichi; Obi, Yoshitsugu; Nagayama, Harumi; Ohno, Motoki; Uematsu, Kazusei; Tamai, Takehiro; Nishide, Takahiro; Rakugi, Hiromi; Isaka, Yoshitaka

    2016-01-01

    There is a paucity of studies on whether early referral (ER) to nephrologist could reduce cardiovascular mortality on dialysis, and the length of pre-dialysis nephrological care needed to reduce mortality on dialysis. A total of 604 consecutive patients who started dialysis between 2001 and 2009 in Senshu region, Osaka, Japan were analyzed. Non-linear associations between mortality and pre-dialysis duration of nephrological care were assessed using restricted cubic spline function, and predictors for death analyzed on Cox modeling. A total of 31.6%, 18.2%, 11.3% and 6.1% of patients had >12, 24, 36 and 48 months of pre-dialysis care, respectively. A total of 258 patients (42.7%) were categorized as ER (≥6 months pre-dialysis duration). During the follow-up period (median, 31.1 months), 218 patients died (cardiovascular, n=70; infection, n=69). Although patients with late referral (LR) had a proxy of inappropriate pre-dialysis care compared with the ER group, Cox multivariate analysis failed to show a favorable association between ER and cardiovascular outcome. In contrast, a deleterious effect of LR on overall survival was observed but was limited only to the first 12 months of dialysis (HR, 1.957; 95% CI: 1.104-3.469; P=0.021), but not observed thereafter. Current pre-dialysis nephrological care may reduce short-term mortality but may not improve cardiovascular mortality after dialysis initiation.

  5. Pollutant emissions and environmental assessment of ethyl 3-ethoxybutyrate, a potential renewable fuel

    DOE PAGES

    Storey, John M. E.; Bunce, Michael P.; Clarke, Edwina M.; ...

    2016-06-14

    Renewable and bio-based transportation fuel sources can lower the life-cycle greenhouse gas emissions from vehicles. Here, we present an initial assessment of ethyl 3-ethoxybutyrate (EEB) as a biofuel in terms of its performance as a fuel oxygenate and its persistence in the environment. EEB can be produced from ethanol and poly-3-hydroxybutyrate, a bacterial storage polymer that can be produced from non-food biomass and other organic feedstocks. The physicochemical properties of EEB and fuel-relevant properties of EEB-gasoline blends were measured, emissions of criteria pollutants from EEB as a gasoline additive in a production vehicle were evaluated, and fate and persistence ofmore » EEB in the environment were estimated. EEB solubility in water was 25.8 g/L, its K ow was 1.8, and its Henry's Law constant was 1.04 x 10 -5 atm-m 3/mole. The anti-knock index values for 5% and 20% v/v EEB-gasoline blends were 91.6 and 91.9, respectively. Reductions in fuel economy were consistent with the level of oxygenation, and criteria emissions were met by the vehicle operated over the urban dynamometer driving cycle (FTP 75). Predicted environmental persistence ranged from 15 d to 30 d which indicates that EEB is not likely to be a persistent organic pollutant. Combined, these results suggest a high potential for the use of EEB as a renewable fuel source.« less

  6. Pollutant emissions and environmental assessment of ethyl 3-ethoxybutyrate, a potential renewable fuel

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Storey, John M. E.; Bunce, Michael P.; Clarke, Edwina M.

    Renewable and bio-based transportation fuel sources can lower the life-cycle greenhouse gas emissions from vehicles. Here, we present an initial assessment of ethyl 3-ethoxybutyrate (EEB) as a biofuel in terms of its performance as a fuel oxygenate and its persistence in the environment. EEB can be produced from ethanol and poly-3-hydroxybutyrate, a bacterial storage polymer that can be produced from non-food biomass and other organic feedstocks. The physicochemical properties of EEB and fuel-relevant properties of EEB-gasoline blends were measured, emissions of criteria pollutants from EEB as a gasoline additive in a production vehicle were evaluated, and fate and persistence ofmore » EEB in the environment were estimated. EEB solubility in water was 25.8 g/L, its K ow was 1.8, and its Henry's Law constant was 1.04 x 10 -5 atm-m 3/mole. The anti-knock index values for 5% and 20% v/v EEB-gasoline blends were 91.6 and 91.9, respectively. Reductions in fuel economy were consistent with the level of oxygenation, and criteria emissions were met by the vehicle operated over the urban dynamometer driving cycle (FTP 75). Predicted environmental persistence ranged from 15 d to 30 d which indicates that EEB is not likely to be a persistent organic pollutant. Combined, these results suggest a high potential for the use of EEB as a renewable fuel source.« less

  7. 'Born in Michigan? You're in the biobank': engaging population biobank participants through Facebook advertisements.

    PubMed

    Platt, J E; Platt, T; Thiel, D; Kardia, S L R

    2013-01-01

    Despite a broad call for biobanks to use social media, data is lacking regarding the capacity of social media tools, especially advertising, to engage large populations on this topic. We used Facebook advertising to engage Michigan residents about the BioTrust for Health. We conducted a low-budget (

  8. Interaction model of steviol glycosides from Stevia rebaudiana (Bertoni) with sweet taste receptors: A computational approach.

    PubMed

    Mayank; Jaitak, Vikas

    2015-08-01

    Docking studies were performed on natural sweeteners from Stevia rebaudiana by constructing homology models of T1R2 and T1R3 subunits of human sweet taste receptors. Ramachandran plot, PROCHECK results and ERRAT overall quality factor were used to validate the quality of models. Furthermore, docking results of steviol glycosides (SG's) were correlated significantly with data available in the literature which enabled to predict the exact sweetness rank order of SG's. The binding pattern indicated that Asn 44, Ans 52, Ala 345, Pro 343, Ile 352, Gly 346, Gly 47, Ala 354, Ser 336, Thr 326 and Ser 329 are the main interacting amino acid residues in case of T1R2 and Arg 56, Glu 105, Asp 215, Asp 216, Glu 148, Asp 258, Lys 255, Ser 104, Glu 217, Leu 51, Arg 52 for T1R3, respectively. Amino acids interact with SG's mainly by forming hydrogen bonds with the hydroxyl group of glucose moieties. Significant variation in docked poses of all the SG's were found. In this study, we have proposed the mechanism of the sweetness of the SG's in the form of multiple point stimulation model by considering the diverse binding patterns of various SG's, as well as their structural features. It will give further insight in understanding the differences in the quality of taste and will be used to improve the taste of SG's using semi-synthetic approaches. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. ‘Born in Michigan? You’re in the Biobank’: Engaging Population Biobank Participants through Facebook Advertisements

    PubMed Central

    Platt, J.E.; Platt, T.; Thiel, D.; Kardia, S.L.R.

    2016-01-01

    Background/Aims Despite a broad call for biobanks to use social media, data is lacking regarding the capacity of social media tools, especially advertising, to engage large populations on this topic. Methods We used Facebook advertising to engage Michigan residents about the BioTrust for Health. We conducted a low-budget (

  10. Liquid-Based Medium Used to Prepare Cytological Breast Nipple Fluid Improves the Quality of Cellular Samples Automatic Collection

    PubMed Central

    Zonta, Marco Antonio; Velame, Fernanda; Gema, Samara; Filassi, Jose Roberto; Longatto-Filho, Adhemar

    2014-01-01

    Background Breast cancer is the second cause of death in women worldwide. The spontaneous breast nipple discharge may contain cells that can be analyzed for malignancy. Halo® Mamo Cyto Test (HMCT) was recently developed as an automated system indicated to aspirate cells from the breast ducts. The objective of this study was to standardize the methodology of sampling and sample preparation of nipple discharge obtained by the automated method Halo breast test and perform cytological evaluation in samples preserved in liquid medium (SurePath™). Methods We analyzed 564 nipple fluid samples, from women between 20 and 85 years old, without history of breast disease and neoplasia, no pregnancy, and without gynecologic medical history, collected by HMCT method and preserved in two different vials with solutions for transport. Results From 306 nipple fluid samples from method 1, 199 (65%) were classified as unsatisfactory (class 0), 104 (34%) samples were classified as benign findings (class II), and three (1%) were classified as undetermined to neoplastic cells (class III). From 258 samples analyzed in method 2, 127 (49%) were classified as class 0, 124 (48%) were classified as class II, and seven (2%) were classified as class III. Conclusion Our study suggests an improvement in the quality and quantity of cellular samples when the association of the two methodologies is performed, Halo breast test and the method in liquid medium. PMID:29147397

  11. The relationship between psychosocial job stress and burnout in emergency departments: an exploratory study.

    PubMed

    García-Izquierdo, Mariano; Ríos-Rísquez, María Isabel

    2012-01-01

    The purpose of this study was to examine the relationship and predictive power of various psychosocial job stressors for the 3 dimensions of burnout in emergency departments. This study was structured as a cross-sectional design, with a questionnaire as the tool. The data were gathered using an anonymous questionnaire in 3 hospitals in Spain. The sample consisted of 191 emergency departments. Burnout was evaluated by the Maslach Burnout Inventory and the job stressors by the Nursing Stress Scale. The Burnout Model in this study consisted of 3 dimensions: emotional exhaustion, cynicism, and reduced professional efficacy. The model that predicted the emotional exhaustion dimension was formed by 2 variables: Excessive workload and lack of emotional support. These 2 variables explained 19.4% of variance in emotional exhaustion. Cynicism had 4 predictors that explained 25.8% of variance: Interpersonal conflicts, lack of social support, excessive workload, and type of contract. Finally, variability in reduced professional efficacy was predicted by 3 variables: Interpersonal conflicts, lack of social support, and the type of shift worked, which explained 10.4% of variance. From the point of view of nurse leaders, organizational interventions, and the management of human resources, this analysis of the principal causes of burnout is particularly useful to select, prioritize, and implement preventive measures that will improve the quality of care offered to patients and the well-being of personnel. Copyright © 2012 Elsevier Inc. All rights reserved.

  12. [Caloric intake in parenteral nutrition of very low weight infants].

    PubMed

    Maggio, L; Gallini, F; De Carolis, M P; Frezza, S; Greco, F

    1994-10-01

    To evaluate the efficacy of a measure able to compare energy intake from parenteral and enteral nutrition we documented growth patterns in a group of VLBW infants treated with parenteral nutrition (PN). To analyze comparative energy intake from the two sources we expressed a percentage of both parenteral and enteral calories: the former (RCP%) related to an optimal value of 85 non protein calories and the latter (RCE%) to an optimal value of 150 total calories. Total energy intake was planned on the RCT% (RCP% + RCE%). We studied 75 VLBW infants with a mean BW of 1040 g and a mean GA of 29.5 weeks. The mean duration of PN was 25.8 +/- 10.4 days. The initial weight loss (10.2 +/- 5.3%), the time to regain BW (5.5 +/- 4 days) and the day of lowest weight (5.2 +/- 1.6 day of life) were in the normal range; the subsequent growth rate resulted 25.9 +/- 9.2 g/kg/die and did not change for different GA or BW. Growth pattern about head circumference and length were above the third percentile. The mean age of RCT% = 100% was 11.4 +/- 4.8 days of PN; this value was higher for the more premature infants. Severe metabolic abnormalities were not detected. Our observations show the efficacy of the RCT% as index of energy from both enteral and parenteral source during PN: the growth pattern seems to be quite satisfactory without any severe metabolic complication.

  13. Anti-Proliferative Effect and Phytochemical Analysis of Cymbopogon citratus Extract

    PubMed Central

    Halabi, Mohammed F.; Sheikh, Bassem Y.

    2014-01-01

    The antiproliferative and antioxidant potential of Cymbopogon citratus (Lemon grass) extracts were investigated. The extracts were isolated by solvent maceration method and thereafter subjected to antiproliferative activity test on five different cancer cells: human colon carcinoma (HCT-116), breast carcinoma (MCF-7 and MDA-MB 231), ovarian carcinoma (SKOV-3 and COAV), and a normal liver cell line (WRL 68). The cell viability was determined using MTT assay. The DPPH radical scavenging assay revealed a concentration dependent trend. A maximum percentage inhibition of 45% and an IC50 of 278 μg/mL were observed when aqueous extract was evaluated. In contrast, 48.3% and IC50 of 258.9 μg/mL were observed when 50% ethanolic extract was evaluated. Both extracts at concentration of 50 to 800 μg/mL showed appreciative metal chelating activity with IC50 value of 172.2 ± 31 μg/mL to 456.5 ± 30 μg/mL. Depending on extraction solvent content, extract obtained from 50% ethanolic solvent proved to be more potent on breast cancer MCF-7 cell line (IC50 = 68 μg/mL). On the other hand, 90% ethanolic extract showed a moderate potency on the ovarian cancer (COAV) and MCF-7 cells having an IC50 of 104.6 μg/mL each. These results suggested antiproliferative efficacy of C. citratus ethanolic extract against human cancer cell lines. PMID:24791006

  14. VizieR Online Data Catalog: Star formation in active and normal galaxies (Tsai+, 2015)

    NASA Astrophysics Data System (ADS)

    Tsai, M.; Hwang, C.-Y.

    2015-11-01

    We selected 104 active galaxies from the lists of Melendez et al. (2010MNRAS.406..493M), Condon et al. 1991 (cat. J/ApJ/378/65), and Ho & Ulvestad 2001 (cat. J/ApJS/133/77). Most of the sources are identified as Active Galactic Nuclei (AGNs), and a few of them are classified as Luminous InfraRed Galaxies (LIRGs). We obtained 3.6 and 8μm infrared images of these galaxies from the Spitzer Archive (http://sha.ipac.caltech.edu/applications/Spitzer/SHA/) and 8GHz images from the VLA archive (http://archive.nrao.edu/archive/archiveimage.html). We also selected a nearby AGN sub-sample containing 21 radio-selected AGNs for further spatial analysis. We selected 25 nearby AGNs exhibiting no detected radio emission in order to compare with the results of the radio-selected sources. For comparison, we also selected normal galaxies with distances less than 15Mpc from the catalog of Tully 1994 (see cat. VII/145). We only selected the galaxies that have Spitzer archive data and are not identified as AGNs in either the Veron-Cetty & Veron 2006 (see cat. VII/258) AGN catalog or in the NED database (http://ned.ipac.caltech.edu/). Our results for the radio-selected and the non-radio-selected active galaxies are listed in Table1, and those for the normal galaxies are listed in Table2. (2 data files).

  15. Pollutant emissions and environmental assessment of ethyl 3-ethoxybutyrate, a potential renewable fuel.

    PubMed

    Storey, John M E; Bunce, Michael P; Clarke, Edwina M; Edmonds, Jennifer W; Findlay, Robert H; Ritchie, Stephen M C; Eyers, Laurent; McMurry, Zackery A; Smoot, James C

    2016-09-01

    Renewable and bio-based transportation fuel sources can lower the life-cycle greenhouse gas emissions from vehicles. We present an initial assessment of ethyl 3-ethoxybutyrate (EEB) as a biofuel in terms of its performance as a fuel oxygenate and its persistence in the environment. EEB can be produced from ethanol and poly-3-hydroxybutyrate, a bacterial storage polymer that can be produced from non-food biomass and other organic feedstocks. Physicochemical properties of EEB and fuel-relevant properties of EEB-gasoline blends were measured, emissions of criteria pollutants from EEB as a gasoline additive in a production vehicle were evaluated, and fate and persistence of EEB in the environment were estimated. EEB solubility in water was 25.8 g/L, its Kow was 1.8, and its Henry's Law constant was 1.04 × 10(-5) atm-m(3)/mole. The anti-knock index values for 5 and 20 % v/v EEB-gasoline blends were 91.6 and 91.9, respectively. Reductions in fuel economy were consistent with the level of oxygenation, and criteria emissions were met by the vehicle operated over the urban dynamometer driving cycle (FTP 75). Predicted environmental persistence ranged from 15 to 30 days which indicates that EEB is not likely to be a persistent organic pollutant. In combination, these results suggest a high potential for the use of EEB as a renewable fuel source.

  16. A pragmatic approach for engineering porous mannitol and mechanistic evaluation of particle performance.

    PubMed

    Al-Khattawi, Ali; Koner, Jasdip; Rue, Peter; Kirby, Dan; Perrie, Yvonne; Rajabi-Siahboomi, Ali; Mohammed, Afzal R

    2015-08-01

    The importance of mannitol has increased recently as an emerging diluent for orodispersible dosage forms. The study aims to prepare spray dried mannitol retaining high porosity and mechanical strength for the development of orally disintegrating tablets (ODTs). Aqueous feed of d-mannitol (10% w/v) comprising ammonium bicarbonate, NH4HCO3 (5% w/v) as pore former was spray dried at inlet temperature of 110-170°C. Compacts were prepared at 151MPa and characterized for porosity, hardness and disintegration time. Particle morphology and drying mechanisms were studied using thermal (HSM, DSC and TGA) and polymorphic (XRD) methods. Tablet porosity increased from 0.20±0.002 for pure mannitol to 0.53±0.03 using fabricated porous mannitol. Disintegration time dropped by 50-77% from 135±5.29s for pure mannitol to 75.33±2.52-31.67±1.53s for mannitol 110-170°C. Hardness increased by 150% at 110°C (258.67±28.89N) and 30% at 150°C (152.70±10.58N) compared to pure mannitol tablets (104.17±1.70N). Increasing inlet temperature resulted in reducing tablet hardness due to generation of 'micro-sponge'-like particles exhibiting significant elastic recovery. Impact of mannitol polymorphism on plasticity/elasticity cannot be ruled out as a mixture of α and β polymorphs formed upon spray drying. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Perceived discrimination and self-rated health in Canada: an exploratory study.

    PubMed

    Du Mont, Janice; Forte, Tonia

    2016-08-08

    Our objective was to explore whether the link between discrimination and self-rated health status differed as a function of discrimination type, including discrimination based on ethnicity/culture, race, physical appearance (other than skin colour), religion, age, and disability. A sample of 19,422 men and women aged 15 and older was included in this study. A multivariate logistic regression analysis was used to measure the association between perceived discrimination types and self-reported health status defined as excellent/good versus fair/poor. The prevalence of experiencing any discrimination in the past five years was higher among those who rated their health as fair or poor (21.8 %) compared to those who rated their health as excellent or good (14.5 %, p < 0.0001). After controlling for all other covariates, there was a positive association between poorer self-rated health and two of the six specific discrimination variables entered into the model: perceived discrimination based on physical appearance (other than skin colour) (OR = 1.79, 95 % CI: 1.24, 2.58) and perceived discrimination based on a having a disability (OR = 1.59, 95 % CI: 1.04, 2.41). Our main findings indicate that perceived discrimination based on physical appearance and disability may have an adverse impact on health. The results highlight the need for a comprehensive approach to improving health outcomes that should include policies that are targeted against specific types of discrimination.

  18. Multiple animal studies for medical chemical defense program in soldier/patient decontamination and drug development. Task order 85-10. Final report, 1 December 1984-1 April 1987

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Joiner, R.L.; Harroff, H.H.; Snider, H.

    1987-12-04

    A rabbit model has been developed and validated for screening noninvasive candidate decontamination systems for their efficacies against topical exposure to the organophosphage chemical surety materiel (CSM), GD, polymer-thickened GD (TGD), and VX. CSM was applied to rabbits in groups of 8 on their clipped dorsa over a range of doses. Dose sites were decontaminated beginning 2 minutes after exposure with both components of the M258A1 standard field kit in the recommended sequence. Replicate LD50s were calculated for each CSM with probit analyses of the doses and lethality rates from replicate studies. A composite LD50 was calculated from the datamore » pooled across replicates for each CSM. The composite LD50 was validated for each CSM by comparing the lethality rate obtained in three replicates of 8 animals each with the population mean of 50 percent. The LD50 values obtained for the three CSM tested produced valid mortality ratios when compared to the population mean. Thus the screen is ready to test candidate decontamination systems. The screen compares the lethality rate obtained from 8 animals each dosed at the established M258A1 LD50 and decontaminated according to the manufacturer's instructions with a candidate system against the population mean of 50 percent. An M258A1-decontaminated control group of 8 animals is included to check for drift via a control chart method. Any candidate decontamination system that is as effective as or more effective than the dual-component M258A1 standard passes the screen and is a candidate for further testing.« less

  19. Prognostic value of mutational characteristics in gastrointestinal stromal tumors: a single-center experience in 275 cases.

    PubMed

    Wang, Ming; Xu, Jia; Zhao, Wenyi; Tu, Lin; Qiu, Weiqing; Wang, Chaojie; Shen, Yangyin; Liu, Qiang; Cao, Hui

    2014-01-01

    The objective of this study was to investigate the impact of KIT/PDGFRA mutations on the prognosis of gastrointestinal stromal tumors (GISTs). In the present study, genotype analyses were performed based on GIST samples from 275 patients. The relationship between mutation and clinicopathological characteristics were explored. All factors were evaluated for their impacts on relapse-free survival (RFS). Briefly, the results of genotype analyses showed that mutations were identified in 258 (93.8 %) patients, and deletion was the most frequent type of mutation accounting for 47.3 % (122/258) of all mutation cases, followed by substitution (87/258, 33.7 %) and duplication (49/258, 19.0 %). Moreover, for KIT exon 11 mutation, the most frequently involved area was from codon 557 to 560. Deep analyses showed that the mutation types were correlated with tumor location (P = 0.005), tumor size (P = 0.022), mitosis rate (P < 0.001), risk grade (P < 0.001), and relapse (P = 0.004). Furthermore, delW557-K558 correlated with mitosis rate (P = 0.042) and relapse (P = 0.036), and delTyr568/570 correlated with tumor origin (P = 0.018). Most importantly, mitotic rate [HR = 2.901 (95 % CI 1.094-7.695), P = 0.032] and risk grade [HR = 9.629 (95 % CI 1.997-46.416), P = 0.005] would be the representative traditional prognostic factors, and deletion with >3 codons would be an novel independent predictor of poor outcome for RFS in GIST patients with deletion mutation of KIT exon 11 [HR = 7.970 (95 % CI 1.774-35.803), P = 0.007]. All results indicated that mutation determined clinicopathological features and prognosis of GISTs, and more than three codons involvement may be a novel adverse indicator.

  20. Active Participation of Cellular Chaperone Hsp90 in Regulating the Function of Rotavirus Nonstructural Protein 3 (NSP3)*

    PubMed Central

    Dutta, Dipanjan; Chattopadhyay, Shiladitya; Bagchi, Parikshit; Halder, Umesh Chandra; Nandi, Satabdi; Mukherjee, Anupam; Kobayashi, Nobumichi; Taniguchi, Koki; Chawla-Sarkar, Mamta

    2011-01-01

    Heat shock protein 90 (Hsp90) has been reported to positively regulate rotavirus replication by modulating virus induced PI3K/Akt and NFκB activation. Here, we report the active association of Hsp90 in the folding and stabilization of rotavirus nonstructural protein 3 (NSP3). In pCD-NSP3-transfected cells, treatment with Hsp90 inhibitor (17-N,N-dimethylethylenediamine-geldanamycin (17DMAG)) resulted in the proteasomal degradation of NSP3. Sequence analysis and deletion mutations revealed that the region spanning amino acids 225–258 within the C-terminal eIF4G-binding domain of NSP3 is a putative Hsp90 binding region. Co-immunoprecipitation and mammalian two-hybrid experiments revealed direct interaction of the C-terminal 12-kDa domain of Hsp90 (C90) with residues 225–258 of NSP3. NSP3-Hsp90 interaction is important for the formation of functionally active mature NSP3, because full-length NSP3 in the presence of the Hsp90 inhibitor or NSP3 lacking the amino acid 225–258 region did not show NSP3 dimers following in vitro coupled transcription-translation followed by chase. Disruption of residues 225–258 within NSP3 also resulted in poor RNA binding and eIF4G binding activity. In addition, inhibition of Hsp90 by 17DMAG resulted in reduced nuclear translocation of poly(A)-binding protein and translation of viral proteins. These results highlight the crucial role of Hsp90 chaperone in the regulation of assembly and functionality of a viral protein during the virus replication and propagation in host cells. PMID:21489987

  1. Predicting the Dynamic Crushing Response of a Composite Honeycomb Energy Absorber Using Solid-Element-Based Models in LS-DYNA

    NASA Technical Reports Server (NTRS)

    Jackson, Karen E.

    2010-01-01

    This paper describes an analytical study that was performed as part of the development of an externally deployable energy absorber (DEA) concept. The concept consists of a composite honeycomb structure that can be stowed until needed to provide energy attenuation during a crash event, much like an external airbag system. One goal of the DEA development project was to generate a robust and reliable Finite Element Model (FEM) of the DEA that could be used to accurately predict its crush response under dynamic loading. The results of dynamic crush tests of 50-, 104-, and 68-cell DEA components are presented, and compared with simulation results from a solid-element FEM. Simulations of the FEM were performed in LS-DYNA(Registered TradeMark) to compare the capabilities of three different material models: MAT 63 (crushable foam), MAT 26 (honeycomb), and MAT 126 (modified honeycomb). These material models are evaluated to determine if they can be used to accurately predict both the uniform crushing and final compaction phases of the DEA for normal and off-axis loading conditions

  2. Comparative toxicities of selected rare earth elements: Sea urchin embryogenesis and fertilization damage with redox and cytogenetic effects.

    PubMed

    Pagano, Giovanni; Guida, Marco; Siciliano, Antonietta; Oral, Rahime; Koçbaş, Fatma; Palumbo, Anna; Castellano, Immacolata; Migliaccio, Oriana; Thomas, Philippe J; Trifuoggi, Marco

    2016-05-01

    Broad-ranging adverse effects are known for rare earth elements (REE), yet only a few studies tested the toxicity of several REE, prompting studies focusing on multi-parameter REE toxicity. Trichloride salts of Y, La, Ce, Nd, Sm, Eu and Gd were tested in Paracentrotus lividus sea urchin embryos and sperm for: (1) developmental defects in either REE-exposed larvae or in the offspring of REE-exposed sperm; (2) fertilization success; (3) mitotic anomalies in REE-exposed embryos and in the offspring of REE-exposed sperm, and (4) reactive oxygen species (ROS) formation, and malondialdehyde (MDA) and nitric oxide (NO) levels. REEs affected P. lividus larvae with concentration-related increase in developmental defects, 10(-6) to 10(-4)M, ranking as: Gd(III)>Y(III)>La(III)>Nd(III)≅Eu(III)>Ce(III)≅Sm(III). Nominal concentrations of REE salts were confirmed by inductively coupled plasma mass spectrometry (ICP-MS). Significant increases in MDA levels, ROS formation, and NO levels were found in REE-exposed embryos. Sperm exposure to REEs (10(-5) to 10(-4)M) resulted in concentration-related decrease in fertilization success along with increase in offspring damage. Decreased mitotic activity and increased aberration rates were detected in REE-exposed embryos and in the offspring of REE-exposed sperm. REE-associated toxicity affecting embryogenesis, fertilization, cytogenetic and redox endpoints showed different activities of tested REEs. Damage to early life stages, along with redox and cytogenetic anomalies should be the focus of future REE toxicity studies. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. Preparation and electrochemical properties of Zr-site substituted Li7La3(Zr2-xMx)O12 (M = Ta, Nb) solid electrolytes

    NASA Astrophysics Data System (ADS)

    Huang, Mian; Shoji, Mao; Shen, Yang; Nan, Ce-Wen; Munakata, Hirokazu; Kanamura, Kiyoshi

    2014-09-01

    Li7La3Zr2O12 (LLZ) solid electrolytes with Zr site partially substituted by Ta and Nb elements were prepared via the conventional solid-state reaction. All the compositions could lead to the cubic garnet-type structure after sintering at 1150 °C. The use of γ-Al2O3 as a sintering aid in the preparation of doped LLZ was studied. It was shown that Al could help to improve the micro-structure for Nb doping, but not necessary for Ta doping. The Ta and Nb doping enhanced the ionic conductivity at 25 °C to 4.09 × 10-4 S cm-1 and 4.50 × 10-4 S cm-1, respectively. A conductivity as high as 1.23 × 10-3 S cm-1 was obtained when measured at 50 °C in air for the Nb-doped LLZ. All-solid-state batteries with LLZTa and LLZNb solid electrolytes were assembled and tested. The cyclic voltammetry (CV) measurement indicated the successful working of the batteries.

  4. SU-F-T-243: Major Risks in Radiotherapy. A Review Based On Risk Analysis Literature

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    López-Tarjuelo, J; Guasp-Tortajada, M; Iglesias-Montenegro, N

    Purpose: We present a literature review of risk analyses in radiotherapy to highlight the most reported risks and facilitate the spread of this valuable information so that professionals can be aware of these major threats before performing their own studies. Methods: We considered studies with at least an estimation of the probability of occurrence of an adverse event (O) and its associated severity (S). They cover external beam radiotherapy, brachytherapy, intraoperative radiotherapy, and stereotactic techniques. We selected only the works containing a detailed ranked series of elements or failure modes and focused on the first fully reported quartile as much.more » Afterward, we sorted the risk elements according to a regular radiotherapy procedure so that the resulting groups were cited in several works and be ranked in this way. Results: 29 references published between 2007 and February 2016 were studied. Publication trend has been generally rising. The most employed analysis has been the Failure mode and effect analysis (FMEA). Among references, we selected 20 works listing 258 ranked risk elements. They were sorted into 31 groups appearing at least in two different works. 11 groups appeared in at least 5 references and 5 groups did it in 7 or more papers. These last sets of risks where choosing another set of images or plan for planning or treating, errors related with contours, errors in patient positioning for treatment, human mistakes when programming treatments, and planning errors. Conclusion: There is a sufficient amount and variety of references for identifying which failure modes or elements should be addressed in a radiotherapy department before attempting a specific analysis. FMEA prevailed, but other studies such as “risk matrix” or “occurrence × severity” analyses can also lead professionals’ efforts. Risk associated with human actions ranks very high; therefore, they should be automated or at least peer-reviewed.« less

  5. DOMAIN DECOMPOSITION METHOD APPLIED TO A FLOW PROBLEM Norberto C. Vera Guzmán Institute of Geophysics, UNAM

    NASA Astrophysics Data System (ADS)

    Vera, N. C.; GMMC

    2013-05-01

    In this paper we present the results of macrohybrid mixed Darcian flow in porous media in a general three-dimensional domain. The global problem is solved as a set of local subproblems which are posed using a domain decomposition method. Unknown fields of local problems, velocity and pressure are approximated using mixed finite elements. For this application, a general three-dimensional domain is considered which is discretized using tetrahedra. The discrete domain is decomposed into subdomains and reformulated the original problem as a set of subproblems, communicated through their interfaces. To solve this set of subproblems, we use finite element mixed and parallel computing. The parallelization of a problem using this methodology can, in principle, to fully exploit a computer equipment and also provides results in less time, two very important elements in modeling. Referencias G.Alduncin and N.Vera-Guzmán Parallel proximal-point algorithms for mixed _nite element models of _ow in the subsurface, Commun. Numer. Meth. Engng 2004; 20:83-104 (DOI: 10.1002/cnm.647) Z. Chen, G.Huan and Y. Ma Computational Methods for Multiphase Flows in Porous Media, SIAM, Society for Industrial and Applied Mathematics, Philadelphia, 2006. A. Quarteroni and A. Valli, Numerical Approximation of Partial Differential Equations, Springer-Verlag, Berlin, 1994. Brezzi F, Fortin M. Mixed and Hybrid Finite Element Methods. Springer: New York, 1991.

  6. Understanding data requirements of retrospective studies.

    PubMed

    Shenvi, Edna C; Meeker, Daniella; Boxwala, Aziz A

    2015-01-01

    Usage of data from electronic health records (EHRs) in clinical research is increasing, but there is little empirical knowledge of the data needed to support multiple types of research these sources support. This study seeks to characterize the types and patterns of data usage from EHRs for clinical research. We analyzed the data requirements of over 100 retrospective studies by mapping the selection criteria and study variables to data elements of two standard data dictionaries, one from the healthcare domain and the other from the clinical research domain. We also contacted study authors to validate our results. The majority of variables mapped to one or to both of the two dictionaries. Studies used an average of 4.46 (range 1-12) data element types in the selection criteria and 6.44 (range 1-15) in the study variables. The most frequently used items (e.g., procedure, condition, medication) are often available in coded form in EHRs. Study criteria were frequently complex, with 49 of 104 studies involving relationships between data elements and 22 of the studies using aggregate operations for data variables. Author responses supported these findings. The high proportion of mapped data elements demonstrates the significant potential for clinical data warehousing to facilitate clinical research. Unmapped data elements illustrate the difficulty in developing a complete data dictionary. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  7. Trace elements in farmed fish (Cyprinus carpio, Ctenopharyngodon idella and Oncorhynchus mykiss) from Beijing: implication from feed.

    PubMed

    Jiang, Haifeng; Qin, Dongli; Mou, Zhenbo; Zhao, Jiwei; Tang, Shizhan; Wu, Song; Gao, Lei

    2016-06-01

    Concentrations of 30 trace elements, Li, V, Cr, Mn, Fe, Ni, Cu, Mo, Zn, Se, Sr, Co, Al, Ti, As, Cs, Sc, Te, Ba, Ga, Pb, Sn, Cd, Sb, Ag, Tm, TI, Be, Hg and U in major cultured freshwater fish species (common carp-Cyprinus carpio, grass carp-Ctenopharyngodon idella and rainbow trout-Oncorhynchus mykiss) with the corresponding feed from 23 fish farms in Beijing, China, were investigated. The results revealed that Fe, Zn, Cu, Mn, Sr, Se were the major accumulated essential elements and Al, Ti were the major accumulated non-essential elements, while Mo, Co, Ga, Sn, Cd, Sb, Ag, Tm, U, TI, Be, Te, Pb and Hg were hardly detectable. Contents of investigated trace elements were close to or much lower than those in fish from other areas in China. Correlation analysis suggested that the elemental concentrations in those fish species were relatively constant and did not vary much with the fish feed. In comparison with the limits for aquafeeds and fish established by Chinese legislation, Cd in 37.5% of rainbow trout feeds and As in 20% of rainbow trout samples exceeded the maximum limit, assuming that inorganic As accounts for 10% of total As. Further health risk assessment showed that fish consumption would not pose risks to consumers as far as non-essential element contaminants are concerned. However, the carcinogenic risk of As in rainbow trout for the inhabitants in Beijing exceeded the acceptable level of 10(-)(4), to which more attention should be paid.

  8. Bottom-Water Conditions in a Marine Basin after the Cretaceous–Paleogene Impact Event: Timing the Recovery of Oxygen Levels and Productivity

    PubMed Central

    Sosa-Montes De Oca, Claudia; Martínez-Ruiz, Francisca; Rodríguez-Tovar, Francisco Javier

    2013-01-01

    An ultra-high-resolution analysis of major and trace element contents from the Cretaceous–Paleogene boundary interval in the Caravaca section, southeast Spain, reveals a quick recovery of depositional conditions after the impact event. Enrichment/depletion profiles of redox sensitive elements indicate significant geochemical anomalies just within the boundary ejecta layer, supporting an instantaneous recovery –some 102 years– of pre-impact conditions in terms of oxygenation. Geochemical redox proxies point to oxygen levels comparable to those at the end of the Cretaceous shortly after impact, which is further evidenced by the contemporary macrobenthic colonization of opportunistic tracemakers. Recovery of the oxygen conditions was therefore several orders shorter than traditional proposals (104–105 years), suggesting a probable rapid recovery of deep-sea ecosystems at bottom and in intermediate waters. PMID:24349232

  9. Decreasing handoff-related care failures in children's hospitals.

    PubMed

    Bigham, Michael T; Logsdon, Tina R; Manicone, Paul E; Landrigan, Christopher P; Hayes, Leslie W; Randall, Kelly H; Grover, Purva; Collins, Susan B; Ramirez, Dana E; O'Guin, Crystal D; Williams, Catherine I; Warnick, Robin J; Sharek, Paul J

    2014-08-01

    Patient handoffs in health care require transfer of information, responsibility, and authority between providers. Suboptimal patient handoffs pose a serious safety risk. Studies demonstrating the impact of improved patient handoffs on care failures are lacking. The primary objective of this study was to evaluate the effect of a multihospital collaborative designed to decrease handoff-related care failures. Twenty-three children's hospitals participated in a quality improvement collaborative aimed at reducing handoff-related care failures. The improvement was guided by evidence-based recommendations regarding handoff intent and content, standardized handoff tools/methods, and clear transition of responsibility. Hospitals tailored handoff elements to locally important handoff types. Handoff-related care failures were compared between baseline and 3 intervention periods. Secondary outcomes measured compliance to specific change package elements and balancing measure of staff satisfaction. Twenty-three children's hospitals evaluated 7864 handoffs over the 12-month study period. Handoff-related care failures decreased from baseline (25.8%) to the final intervention period (7.9%) (P < .05). Significant improvement was observed in every handoff type studied. Compliance to change package elements improved (achieving a common understanding about the patient from 86% to 96% [P < .05]; clear transition of responsibility from 92% to 96% [P < .05]; and minimized interruptions and distractions from 84% to 90% [P < .05]) as did overall satisfaction with the handoff (from 55% to 70% [P < .05]). Implementation of a standardized evidence-based handoff process across 23 children's hospitals resulted in a significant decrease in handoff-related care failures, observed over all handoff types. Compliance to critical components of the handoff process improved, as did provider satisfaction. Copyright © 2014 by the American Academy of Pediatrics.

  10. Results of elemental and stable isotopic measurements, and dietary composition of Arctic grayling (Thymallus arcticus) collected in 2000 and 2001 from the Fortymile River Watershed, Alaska

    USGS Publications Warehouse

    Crock, J.G.; Seal, R.R.; Gough, L.P.; Weber-Scannell, P.

    2003-01-01

    We report the results of the elemental and stable isotopic analyses, as well as the composition of stomach contents, of Arctic grayling (Thymallus arcticus), an ecologically important resident freshwater sport and subsistence fish in the Fortymile River Mining District of the Interior Highlands Ecoregion in eastern Alaska. These data are presented here as a data compilation with minimal interpretation or discussion. Further analyses of the data will be presented elsewhere. The study area has been mined for placer gold for over a century and is currently experiencing renewed mineral exploration activity. The results for the analysis of 40 inorganic elements are reported for grayling muscle (fillet) tissue, liver tissue, and stomach contents from 34 individuals caught at 11 sites within the watershed. The 11 sites were classified as occurring within the following lithologies: metavolcanic (7 sites), metasedimentary (3 sites), and granitic intrusion (1 site). This information (along with fish tissue stable isotope data) is critical in the assessment of the influence of regional lithology on the fish chemical composition, especially the trace metal content. We report the nitrogen, carbon, and sulfur stable isotope composition of muscle samples. Nitrogen isotopes appear homogeneous (d15N = 7.6 to 9.7 permil) whereas carbon and sulfur isotope compositions of the same samples span a range from d 13C = ?33.1 to ?25.8 permil, and d 34S = ?8.4 to 8.2 permil. Stomach content material was examined for the occurrence and frequency of macroinvertebrate composition and diversity in three individual fish. Results showed a high degree of diversity with 9 to 15 invertebrate taxa; both aquatic and terrestrial forms were represented.

  11. Uncertainty analysis of depth predictions from seismic reflection data using Bayesian statistics

    NASA Astrophysics Data System (ADS)

    Michelioudakis, Dimitrios G.; Hobbs, Richard W.; Caiado, Camila C. S.

    2018-06-01

    Estimating the depths of target horizons from seismic reflection data is an important task in exploration geophysics. To constrain these depths we need a reliable and accurate velocity model. Here, we build an optimum 2-D seismic reflection data processing flow focused on pre-stack deghosting filters and velocity model building and apply Bayesian methods, including Gaussian process emulation and Bayesian History Matching, to estimate the uncertainties of the depths of key horizons near the Deep Sea Drilling Project (DSDP) borehole 258 (DSDP-258) located in the Mentelle Basin, southwest of Australia, and compare the results with the drilled core from that well. Following this strategy, the tie between the modelled and observed depths from DSDP-258 core was in accordance with the ±2σ posterior credibility intervals and predictions for depths to key horizons were made for the two new drill sites, adjacent to the existing borehole of the area. The probabilistic analysis allowed us to generate multiple realizations of pre-stack depth migrated images, these can be directly used to better constrain interpretation and identify potential risk at drill sites. The method will be applied to constrain the drilling targets for the upcoming International Ocean Discovery Program, leg 369.

  12. Radio observations of a few selected blazars

    NASA Technical Reports Server (NTRS)

    Saikia, D. J.; Salter, C. J.; Neff, S. G.; Gower, A. C.; Sinha, R. P.

    1987-01-01

    The paper presents total-intensity and linear-polarization observations of four selected blazars, 0716+714, 0752+258, 1156+295 and 1400+162, with the VLA A-array, and MERLIN and EVN observations of 1400+162. The sources 0752+258 and 1400+162 which have nearly constant optical polarization, have well-defined double-lobed radio structure, with relatively weak radio cores, and are likely to be at large viewing angles. In addition, 0752+258 appears to be a twin-jet blazar. The position angle (PA) of the VLBI jet in 1400+162 is close to that of the arcsec-scale jet near the nucleus, as well as the optical and 2-cm core polarization PAs. The blazars 0716+714 and 1156+295, which exhibit strongly variable optical polarization, have a core-dominated radio structure and perhaps have their jet axes close to the line-of-sight. From polarization observations at 20, 18, 6, and 2 cm, it is found that the rotation measure of the radio core in 0716+714 is about -20 rad/sq m. It is suggested that low values of core rotation measure in core-dominated sources could be consistent with the relativistic beaming models.

  13. The arsenic hyperaccumulating Pteris vittata expresses two arsenate reductases

    NASA Astrophysics Data System (ADS)

    Cesaro, Patrizia; Cattaneo, Chiara; Bona, Elisa; Berta, Graziella; Cavaletto, Maria

    2015-09-01

    Enzymatic reduction of arsenate to arsenite is the first known step in arsenate metabolism in all organisms. Although the presence of one mRNA arsenate reductase (PvACR2) has been characterized in gametophytes of P. vittata, no arsenate reductase protein has been directly observed in this arsenic hyperaccumulating fern, yet. In order to assess the possible presence of arsenate reductase in P. vittata, two recombinant proteins, ACR2-His6 and Trx-His6-S-Pv2.5-8 were prepared in Escherichia coli, purified and used to produce polyclonal antibodies. The presence of these two enzymes was evaluated by qRT-PCR, immunoblotting and direct MS analysis. Enzymatic activity was detected in crude extracts. For the first time we detected and identified two arsenate reductase proteins (PvACR2 and Pv2.5-8) in sporophytes and gametophytes of P. vittata. Despite an increase of the mRNA levels for both proteins in roots, no difference was observed at the protein level after arsenic treatment. Overall, our data demonstrate the constitutive protein expression of PvACR2 and Pv2.5-8 in P. vittata tissues and propose their specific role in the complex metabolic network of arsenic reduction.

  14. A role for Tn6029 in the evolution of the complex antibiotic resistance gene loci in genomic island 3 in enteroaggregative hemorrhagic Escherichia coli O104:H4.

    PubMed

    Roy Chowdhury, Piklu; Charles, Ian G; Djordjevic, Steven P

    2015-01-01

    In enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104 the complex antibiotic resistance gene loci (CRL) found in the region of divergence 1 (RD1) within E. coli genomic island 3 (GI3) contains blaTEM-1, strAB, sul2, tet(A)A, and dfrA7 genes encoding resistance to ampicillin, streptomycin, sulfamethoxazole, tetracycline and trimethoprim respectively. The precise arrangement of antibiotic resistance genes and the role of mobile elements that drove the evolutionary events and created the CRL have not been investigated. We used a combination of bioinformatics and iterative BLASTn searches to determine the micro-evolutionary events that likely led to the formation of the CRL in GI3 using the closed genome sequences of EAHEC O104:H4 strains 2011C-3493 and 2009EL-2050 and high quality draft genomes of EAHEC E. coli O104:H4 isolates from sporadic cases not associated with the initial outbreak. Our analyses indicate that the CRL in GI3 evolved from a progenitor structure that contained an In2-derived class 1 integron in a Tn21/Tn1721 hybrid backbone. Within the hybrid backbone, a Tn6029-family transposon, identified here as Tn6029C abuts the sul1 gene in the 3'-Conserved Segment (-CS) of a class 1 integron generating a unique molecular signature that has only previously been observed in pASL01a, a small plasmid found in commensal E. coli in West Africa. From this common progenitor, independent IS26-mediated events created two novel transposons identified here as Tn6029D and Tn6222 in 2011C-3493 and 2009EL-2050 respectively. Analysis of RD1 within GI3 reveals IS26 has played a crucial role in the assembly of regions within the CRL.

  15. Mutant Analysis Reveals Allosteric Regulation of ClpB Disaggregase

    PubMed Central

    Franke, Kamila B.; Bukau, Bernd; Mogk, Axel

    2017-01-01

    The members of the hexameric AAA+ disaggregase of E. coli and S. cerevisiae, ClpB, and Hsp104, cooperate with the Hsp70 chaperone system in the solubilization of aggregated proteins. Aggregate solubilization relies on a substrate threading activity of ClpB/Hsp104 fueled by ATP hydrolysis in both ATPase rings (AAA-1, AAA-2). ClpB/Hsp104 ATPase activity is controlled by the M-domains, which associate to the AAA-1 ring to downregulate ATP hydrolysis. Keeping M-domains displaced from the AAA-1 ring by association with Hsp70 increases ATPase activity due to enhanced communication between protomers. This communication involves conserved arginine fingers. The control of ClpB/Hsp104 activity is crucial, as hyperactive mutants with permanently dissociated M-domains exhibit cellular toxicity. Here, we analyzed AAA-1 inter-ring communication in relation to the M-domain mediated ATPase regulation, by subjecting a conserved residue of the AAA-1 domain subunit interface of ClpB (A328) to mutational analysis. While all A328X mutants have reduced disaggregation activities, their ATPase activities strongly differed. ClpB-A328I/L mutants have reduced ATPase activity and when combined with the hyperactive ClpB-K476C M-domain mutation, suppress cellular toxicity. This underlines that ClpB ATPase activation by M-domain dissociation relies on increased subunit communication. The ClpB-A328V mutant in contrast has very high ATPase activity and exhibits cellular toxicity on its own, qualifying it as novel hyperactive ClpB mutant. ClpB-A328V hyperactivity is however, different from that of M-domain mutants as M-domains stay associated with the AAA-1 ring. The high ATPase activity of ClpB-A328V primarily relies on the AAA-2 ring and correlates with distinct conformational changes in the AAA-2 catalytic site. These findings characterize the subunit interface residue A328 as crucial regulatory element to control ATP hydrolysis in both AAA rings. PMID:28275610

  16. Elemental calcium intake associated with calcium acetate/calcium carbonate in the treatment of hyperphosphatemia

    PubMed Central

    Wilson, Rosamund J; Copley, J Brian

    2017-01-01

    Background Calcium-based and non-calcium-based phosphate binders have similar efficacy in the treatment of hyperphosphatemia; however, calcium-based binders may be associated with hypercalcemia, vascular calcification, and adynamic bone disease. Scope A post hoc analysis was carried out of data from a 16-week, Phase IV study of patients with end-stage renal disease (ESRD) who switched to lanthanum carbonate monotherapy from baseline calcium acetate/calcium carbonate monotherapy. Of the intent-to-treat population (N=2520), 752 patients with recorded dose data for calcium acetate (n=551)/calcium carbonate (n=201) at baseline and lanthanum carbonate at week 16 were studied. Elemental calcium intake, serum phosphate, corrected serum calcium, and serum intact parathyroid hormone levels were analyzed. Findings Of the 551 patients with calcium acetate dose data, 271 (49.2%) had an elemental calcium intake of at least 1.5 g/day at baseline, and 142 (25.8%) had an intake of at least 2.0 g/day. Mean (95% confidence interval [CI]) serum phosphate levels were 6.1 (5.89, 6.21) mg/dL at baseline and 6.2 (6.04, 6.38) mg/dL at 16 weeks; mean (95% CI) corrected serum calcium levels were 9.3 (9.16, 9.44) mg/dL and 9.2 (9.06, 9.34) mg/dL, respectively. Of the 201 patients with calcium carbonate dose data, 117 (58.2%) had an elemental calcium intake of at least 1.5 g/day, and 76 (37.8%) had an intake of at least 2.0 g/day. Mean (95% CI) serum phosphate levels were 5.8 (5.52, 6.06) mg/dL at baseline and 5.8 (5.53, 6.05) mg/dL at week 16; mean (95% CI) corrected serum calcium levels were 9.7 (9.15, 10.25) mg/dL and 9.2 (9.06, 9.34) mg/dL, respectively. Conclusion Calcium acetate/calcium carbonate phosphate binders, taken to control serum phosphate levels, may result in high levels of elemental calcium intake. This may lead to complications related to calcium balance. PMID:28182142

  17. Miniature Transposable Sequences Are Frequently Mobilized in the Bacterial Plant Pathogen Pseudomonas syringae pv. phaseolicola

    PubMed Central

    Bardaji, Leire; Añorga, Maite; Jackson, Robert W.; Martínez-Bilbao, Alejandro; Yanguas-Casás, Natalia; Murillo, Jesús

    2011-01-01

    Mobile genetic elements are widespread in Pseudomonas syringae, and often associate with virulence genes. Genome reannotation of the model bean pathogen P. syringae pv. phaseolicola 1448A identified seventeen types of insertion sequences and two miniature inverted-repeat transposable elements (MITEs) with a biased distribution, representing 2.8% of the chromosome, 25.8% of the 132-kb virulence plasmid and 2.7% of the 52-kb plasmid. Employing an entrapment vector containing sacB, we estimated that transposition frequency oscillated between 2.6×10−5 and 1.1×10−6, depending on the clone, although it was stable for each clone after consecutive transfers in culture media. Transposition frequency was similar for bacteria grown in rich or minimal media, and from cells recovered from compatible and incompatible plant hosts, indicating that growth conditions do not influence transposition in strain 1448A. Most of the entrapped insertions contained a full-length IS801 element, with the remaining insertions corresponding to sequences smaller than any transposable element identified in strain 1448A, and collectively identified as miniature sequences. From these, fragments of 229, 360 and 679-nt of the right end of IS801 ended in a consensus tetranucleotide and likely resulted from one-ended transposition of IS801. An average 0.7% of the insertions analyzed consisted of IS801 carrying a fragment of variable size from gene PSPPH_0008/PSPPH_0017, showing that IS801 can mobilize DNA in vivo. Retrospective analysis of complete plasmids and genomes of P. syringae suggests, however, that most fragments of IS801 are likely the result of reorganizations rather than one-ended transpositions, and that this element might preferentially contribute to genome flexibility by generating homologous regions of recombination. A further miniature sequence previously found to affect host range specificity and virulence, designated MITEPsy1 (100-nt), represented an average 2.4% of the total number of insertions entrapped in sacB, demonstrating for the first time the mobilization of a MITE in bacteria. PMID:22016774

  18. Miniature transposable sequences are frequently mobilized in the bacterial plant pathogen Pseudomonas syringae pv. phaseolicola.

    PubMed

    Bardaji, Leire; Añorga, Maite; Jackson, Robert W; Martínez-Bilbao, Alejandro; Yanguas-Casás, Natalia; Murillo, Jesús

    2011-01-01

    Mobile genetic elements are widespread in Pseudomonas syringae, and often associate with virulence genes. Genome reannotation of the model bean pathogen P. syringae pv. phaseolicola 1448A identified seventeen types of insertion sequences and two miniature inverted-repeat transposable elements (MITEs) with a biased distribution, representing 2.8% of the chromosome, 25.8% of the 132-kb virulence plasmid and 2.7% of the 52-kb plasmid. Employing an entrapment vector containing sacB, we estimated that transposition frequency oscillated between 2.6×10(-5) and 1.1×10(-6), depending on the clone, although it was stable for each clone after consecutive transfers in culture media. Transposition frequency was similar for bacteria grown in rich or minimal media, and from cells recovered from compatible and incompatible plant hosts, indicating that growth conditions do not influence transposition in strain 1448A. Most of the entrapped insertions contained a full-length IS801 element, with the remaining insertions corresponding to sequences smaller than any transposable element identified in strain 1448A, and collectively identified as miniature sequences. From these, fragments of 229, 360 and 679-nt of the right end of IS801 ended in a consensus tetranucleotide and likely resulted from one-ended transposition of IS801. An average 0.7% of the insertions analyzed consisted of IS801 carrying a fragment of variable size from gene PSPPH_0008/PSPPH_0017, showing that IS801 can mobilize DNA in vivo. Retrospective analysis of complete plasmids and genomes of P. syringae suggests, however, that most fragments of IS801 are likely the result of reorganizations rather than one-ended transpositions, and that this element might preferentially contribute to genome flexibility by generating homologous regions of recombination. A further miniature sequence previously found to affect host range specificity and virulence, designated MITEPsy1 (100-nt), represented an average 2.4% of the total number of insertions entrapped in sacB, demonstrating for the first time the mobilization of a MITE in bacteria.

  19. F-104 in flight

    NASA Technical Reports Server (NTRS)

    1988-01-01

    F-104G (N826NA) in flight over the Mojave Desert in January 1988. This aircraft was the last of eleven F-104s delivered to the Dryden Flight Research Center over a period of four decades. The initial group of four (a YF-104A, two F-104As and a two-seat F-104B) arrived between August 1956 and December 1959. One of the F-104As was returned to the Air Force in 1961, and the other was lost in a non-fatal accident in 1962. To support X-15 activities, three special F-104Ns went to NASA in 1963. One crashed in the XB-70 midair collision, and it was replaced by an F-104A/G. (This was an F-104A modified to a G configuration.) As the initial F-104 fleet aged, a pair of two-seat TF-104Gs and a single-seat F-104G joined the Dryden inventory in June 1975. F-104G N826NA, shown in the photo, was one of these. Between 1975 and 1990, the older F-104s were retired - the YF-104A in November 1975, the F-104A/G in June 1977, the F-104B in June 1983, and the two F-104Ns in January 1987 and October 1990. As the F-104s phased out, the replacement F-18s started arriving at Dryden in 1984. F-104s N826NA made its 1,415th and last flight on February 3, 1994. The last two TF-104s ended service in September 1995, ending a 39 year involvement with the aircraft by the NACA and NASA.

  20. Evaluation of Trace Elements and Their Relationship with Growth and Development of Young Children.

    PubMed

    Cao, Jia; Gao, Zhenyan; Yan, Jin; Li, Minming; Su, Jia; Xu, Jian; Yan, Chong-Huai

    2016-06-01

    This study was conducted to assess the levels of trace elements and their relationship with growth and development of children in Shanghai, China, to offer scientific evidence for supplementing trace elements in children. A stratified, clustered, random sampling method was used in the study. Blood samples were taken from 2141 Shanghai children from 0 to 6.0 years old, and the concentrations of zinc (Zn), calcium (Ca), iron (Fe), copper (Cu), and magnesium (Mg) were measured using inductively coupled plasma mass spectrometry (ICP-MS). Nutritional status was determined and Z-scores of anthropometric parameters, such as height for age (HFA), weight for age (WFA), and body mass index (BMI) were calculated, indicated by HAZ, WAZ, and BMIZ, respectively. The overall median blood levels of Zn, Ca, Fe, Cu, and Mg were 8.83, 79.02, 9.49, 1.04, and 15.45 mg/L, respectively. Fe, Cu, and Mg increased with age and Zn, Fe, and Cu differed by sex. HAZ and WAZ were positively correlated with Zn (r (2) = 0.072 and 0.053, respectively; P < 0.05). Trace elements were significantly related to children's growth and development. Dietary supplementation and screening of nutritional states are potential solutions to improve children's growth and development.

  1. The Effect of Rapid Liquid-Phase Reactions on Injector Design and Combustion in Rocket Motors

    NASA Technical Reports Server (NTRS)

    Elverum, Gerard W., Jr.; Staudhammer, Peter

    1959-01-01

    Data are presented indicating the rates and magnitudes of energy released by the liquid-phase reactions of various propellant combinations. The data show that this energy release can contribute significantly to the rate of vaporization of the incoming propellants and thus aid the combustion process. Nevertheless, very low performances were obtained in rocket motors with conventional impinging-jet injectors when highly reactive systems such as N104-N2H4, were employed. A possible explanation for this low performance is that the initial reactions of such systems are so rapid that liquid-phase mixing is inhibited. Evidence for such an effect is presented in a series of color photographs of open flames using various injector elements. Based on these studies, some requirements are suggested for injector elements using highly reactive propellants. Experimental results are presented of motor tests using injector elements in which some of these requirements are met through the use of a set of concentric tubes. These tests, carried out at thrust levels of 40 to 800 lb per element, demonstrated combustion efficiencies of up to 98% based on equilibrium characteristic velocity values. Results are also presented for tests made with impinging-jet and splash-plate injectors for comparison.

  2. [Patient satisfaction in a laboratory test collection unit].

    PubMed

    de Moura, Gisela Maria Schebella Souto; Hilleshein, Eunice Fabiani; Schardosim, Juliana Machado; Delgado, Kátia Simone

    2008-06-01

    This exploratory descriptive study aimed at identifying customer satisfaction attributes in the field of laboratory tests. Data were collected in 2006, using 104 interviews in a laboratorial unit inside a teaching hospital, using the critical incident technique, and submitted to content analysis. Three attribute categories were identified: time spent in waiting for care, interpersonal contact, and technical skills. These results subsidize the assessment of the current satisfaction survey tool, and point to its reformulation. They also allow the identification of improvement needs in customer attention, and provide elements to be taken into account in personnel selection, training programs, personnel performance assessment.

  3. What were they thinking? Men who murder an intimate partner.

    PubMed

    Dobash, R Emerson; Dobash, Russell P

    2011-01-01

    The focus is on cognitions of men who murder an intimate partner and includes thinking prior to and after the murder. Based on the Murder in Britain Study, the qualitative accounts of various professionals included in the case-files of 104 men convicted of murdering a woman partner are used to examine beliefs about intimate relationships, orientations toward violence and previous violence to the victim, as well as subsequent denials, rationalizations, and justifications. We conclude that these and other cognitions are important elements of intimate partner murder and must be challenged and changed in efforts to eliminate nonlethal abuse and murder.

  4. Computer-Aided Structural Engineering (CASE) Project. Finite Element Studies of a Horizontally Framed Miter Gate. Report 7. Application and Summary.

    DTIC Science & Technology

    1987-08-01

    gate torsion is included in Part IV. The complete list of reports involved in the miter gate study can be found on page I of the preface. 17 . Before...Fig- ure 10. SM and CM results do not match. However, the pattern of distribution is similar; lower girders 17 and 18 carry high compression loads...118 (0.87) 142 (1.04) Girder 15 78 (0.57) 33 (0.24) Girder 16 -44 (-0.32) -30 (-0.22) Girder 17 -106 (-0.78) -147 (-1.08) Girder 18 -182 (-1.34) -134

  5. Elemental composition and sources of fine and ultrafine ambient particles in Erfurt, Germany.

    PubMed

    Cyrys, J; Stölzel, M; Heinrich, J; Kreyling, W G; Menzel, N; Wittmaack, K; Tuch, T; Wichmann, H-Erich

    2003-04-15

    We present the first results of a source apportionment for the urban aerosol in Erfurt, Germany, for the period 1995-1998. The analysis is based on data of particle number concentrations (0.01-2.5 microm; mean 1.8 x 10(4) cm(-3), continuous), the concentration of the ambient gases SO(2), NO, NO(2) and CO (continuous), particle mass less than 2.5 microm (PM(2.5)) and less than 10 microm (PM(10)) (Harvard Impactor sampling, mean PM(2.5) 26.3 micro/m(3), mean PM(10) 38.2 microg/m(3)) and the size fractionated concentrations of 19 elements (impactor sampling 0.05-1.62 microm, PIXE analysis). We determined: (a) the correlations between (i) the 1- and 24-h average concentrations of the gaseous pollutants and the particle number as well as the particle mass concentration and (ii) between the 24-h elemental concentrations; (b) Crustal Enrichment Factors for the PIXE elements using Si as reference element; and (c) the diurnal pattern of the measured pollutants on weekdays and on weekends. The highly correlated PIXE elements Si, Al, Ti and Ca having low enrichment factors were identified as soil elements. The strong correlation of particle number concentrations with NO, which is considered to be typically emitted by traffic, and the striking similarity of their diurnal variation suggest that a sizable fraction of the particle number concentration is associated with emission from vehicles. Besides NO and particle number concentrations other pollutants such as NO(2), CO as well as the elements Zn and Cu were strongly correlated and appear to reflect motor vehicle traffic. Sulfur could be a tracer for coal combustion, however, it was not correlated with any of the quoted elements. Highly correlated elements V and Ni have similar enrichment factors and are considered as tracers for oil combustion.

  6. Crystal structures of Hsp104 N-terminal domains from Saccharomyces cerevisiae and Candida albicans suggest the mechanism for the function of Hsp104 in dissolving prions

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wang, Peng; Li, Jingzhi; Weaver, Clarissa

    Hsp104 is a yeast member of the Hsp100 family which functions as a molecular chaperone to disaggregate misfolded polypeptides. To understand the mechanism by which the Hsp104 N-terminal domain (NTD) interacts with its peptide substrates, crystal structures of the Hsp104 NTDs fromSaccharomyces cerevisiae(ScHsp104NTD) andCandida albicans(CaHsp104NTD) have been determined at high resolution. The structures of ScHsp104NTD and CaHsp104NTD reveal that the yeast Hsp104 NTD may utilize a conserved putative peptide-binding groove to interact with misfolded polypeptides. In the crystal structures ScHsp104NTD forms a homodimer, while CaHsp104NTD exists as a monomer. The consecutive residues Gln105, Gln106 and Lys107, and Lys141 around themore » putative peptide-binding groove mediate the monomer–monomer interactions within the ScHsp104NTD homodimer. Dimer formation by ScHsp104NTD suggests that the Hsp104 NTD may specifically interact with polyQ regions of prion-prone proteins. The data may reveal the mechanism by which Hsp104 NTD functions to suppress and/or dissolve prions.« less

  7. Vertical distribution of potentially toxic elements in sediments impacted by intertidal geothermal hot springs (Bahia Concepcion, Gulf of California)

    NASA Astrophysics Data System (ADS)

    Leal-Acosta, M. L.; Shumilin, E.

    2016-12-01

    The intertidal geothermal hot springs (GHS) in Bahia Concepcion, Gulf of California are the source of potentially toxic elements to the adjacent marine environment surrounded by mangroves trees. The anoxic sediments enriched in organic carbon accumulate As, Hg and other heavy metals that can be bioavailable for the biota. To know the vertical distribution of these elements the geochemistry of a short sediment core was carried out. It was collected in June, 2010 in the mangrove area near to GHS (1 m) during a low tide, pushing manually a polypropylene tube into the sediments. The extracted sediment core was cut with plastic knife on 1 cm thick sub-samples, stored in plastic bags and transported on ice to the laboratory. The major and trace elements contents were determinate by ICP-MS after total digestion with stronger acids (HClO4-HNO3-HCl-HF). Certificate reference materials were used for the quality control of the method obtaining good recoveries for most of the elements (80-105%). The sediment core had high maximum contents of CaCO3 (70%) and total organic carbon (12%). The concentration of Hg along the core ranges from 650 to 74300 mg kg-1 and had more than three orders of magnitude above the reference values of 40 mg kg-1 for the Upper Continental Crust (UCC)1. In contrast, As ranges from 12 to 258 mg kg-1 resulting in more than one order of magnitude respect to UCC1 (1.7 mg kg-1). Similar pattern result for Mn, Cu, Pb, and Zn with the maximum values of 3200 mg kg-1, 42 mg kg-1, 12.4 mg kg-1, 71 mg kg-1 respectively that coincide with the maximum for As at the same core depth (4 cm). The Ca, Li, Co, Sb, U, and Mg also show high contents in comparison with the UCC1reference values. The maximum contents of Mo and Cd coincide with maximum concentration of sulfur (2%) at 6 to 8 cm. The enrichment factor calculated using Al as normalizing element showed Cd (7-280), As (26-329) and Hg (23-1196) as highly enriched mainly in the first centimeters of the sediment core. 1 Wedephol (1995)

  8. 18 CFR 385.104 - Rule of construction (Rule 104).

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Rule of construction (Rule 104). 385.104 Section 385.104 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY... Definitions § 385.104 Rule of construction (Rule 104). To the extent that the text of a rule is inconsistent...

  9. 18 CFR 385.104 - Rule of construction (Rule 104).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Rule of construction (Rule 104). 385.104 Section 385.104 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY... Definitions § 385.104 Rule of construction (Rule 104). To the extent that the text of a rule is inconsistent...

  10. Metals exposure and risk of small-for-gestational age birth in a Canadian birth cohort: The MIREC study

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Thomas, Shari; Arbuckle, Tye E., E-mail: Tye.Arbuckle@hc-sc.gc.ca; Fisher, Mandy

    Background: Lead, mercury, cadmium and arsenic are some of the most common toxic metals to which Canadians are exposed. The effect of exposure to current low levels of toxic metals on fetal growth restriction is unknown. Objective: The aim of this study was to examine relationships between exposure to lead, mercury, cadmium and arsenic during pregnancy, and risk of small for gestational age (SGA) birth. Methods: Lead, mercury, cadmium and arsenic levels were measured in blood samples from the first and third trimesters in 1835 pregnant women from across Canada. Arsenic species in first trimester urine were also assessed. Relativemore » risks and 95% confidence intervals were estimated using log binomial multivariate regression. Important covariates including maternal age, parity, pre-pregnancy BMI, and smoking, were considered in the analysis. An exploratory analysis was performed to examine potential effect modification of these relationships by single nucleotide polymorphisms (SNPs) in GSTP1 and GSTO1 genes. Results: No association was found between blood lead, cadmium or arsenic and risk for SGA. We observed an increased risk for SGA for the highest compared to the lowest tertile of exposure for mercury (>1.6 µg/L, RR=1.56.; 95% CI=1.04–2.58) and arsenobetaine (>2.25 µg/L, RR=1.65; 95% CI=1.10–2.47) after adjustment for the effects of parity and smoking. A statistically significant interaction was observed in the relationship between dimethylarsinic acid (DMA) levels in urinary arsenic and SGA between strata of GSTO1 A104A (p for interaction=0.02). A marginally significant interaction was observed in the relationship between blood lead and SGA between strata of GSTP1 A114V (p for interaction=0.06). Conclusions: These results suggest a small increase in risk for SGA in infants born to women exposed to mercury and arsenic. Given the conflicting evidence in the literature this warrants further investigation in other pregnant populations. - Highlights: • Metals measured in blood or urine of pregnant women. • No association between blood lead, cadmium or arsenic and SGA. • Small increased risk for highest tertile of blood mercury. • Small increased risk for highest tertile of urinary arsenobetaine. • Further research needed to confirm observed associations.« less

  11. Energy, nutrient and food content of snacks in French adults.

    PubMed

    Si Hassen, Wendy; Castetbon, Katia; Tichit, Christine; Péneau, Sandrine; Nechba, Anouar; Ducrot, Pauline; Lampuré, Aurélie; Bellisle, France; Hercberg, Serge; Méjean, Caroline

    2018-02-27

    Snacking raises concern since it may lead to an additional energy intake and poor nutrient quality. A snacking occasion can be defined as any eating occasion apart from main meals, regardless of the amount or type of foods consumed. We described the frequency of snacking occasions according to daily timing in French adults, and compared them between each other, and with the main meals, in terms of energy intake, energy and nutrient density, and food content. This cross-sectional analysis included 104,265 adults from the NutriNet-Santé cohort. Food intake was estimated using 24-h records of weekdays. For each eating occasion, nutrient density and energy content and density were computed. After weighting, 47.6% of our sample were men and mean age was 45.6 (15.3). Overall, 68% of participants ate at least one snack during the reported record, mainly in the morning or afternoon. Overall snack had a lower nutrient density [22.8 (SD = 278.3)] than main meals [25.8 (36.9) to 30.0 (30.4)]; but higher energy density [222.2 (163.3) kcal/100 g] than meals [133.9 (57.3) to 175.9 (99.6) kcal/100 g]. Morning snack was the snacking occasion with the lowest energy density [211 kcal/100 g], the lowest energy intake [104.1 kcal] and the highest nutrient density [60.1]. Afternoon and evening snacks had the highest energy loads [192.4 kcal and 207.6 kcal], but low nutrient scores [16 and 13, respectively]. The main food groups contributing to energy intake from snacks were fatty-sweet and sugary foods, fruit, hot beverages, and bread. Our findings highlight the frequency of snacking and the varying nutritional quality of snacks over the day. The morning snack was shown to be healthier than afternoon and evening snacks. This study was conducted according to guidelines laid down in the Declaration of Helsinki, and all procedures were approved by the Institutional Review Board of the French Institute for Health and Medical Research (IRB Inserm No. 0000388FWA00005831) and the French Data Protection Authority (Commission Nationale Informatique et Libertés No. 908450 and No. 909216). Electronic informed consent was obtained from all participants (Clinical Trial no. NCT03335644 ).

  12. Effect of urea and urea-gamma treatments on cellulose degradation of Thai rice straw and corn stalk

    NASA Astrophysics Data System (ADS)

    Banchorndhevakul, Siriwattana

    2002-08-01

    Cellulose degradation of 20% urea treated and 20% urea-10 kGy gamma treated Thai rice straw and corn stalk showed that combination effect of urea and gamma radiation gave a higher % decrease in neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), cellulose, hemicellulose, and lignin and cutin in comparison with urea effect only for both room temperature storage and room temperature +258 K storage. The results also indicated that cellulose degradation proceeded with time, even at 258 K. A drastic drop to less than half of the original contents in NDF, ADF, and ADL could not be obtained in this study.

  13. Synthesis, Characterization, and BSA-Binding Studies of Novel Sulfonated Zinc-Triazine Complexes

    PubMed Central

    Abeydeera, Nalin; Perera, Inoka C.

    2018-01-01

    Four Zn(II) complexes containing a pyridyl triazine core (L1 = 3-(2-pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5″-disulfonic acid disodium salt and L2 = 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4″-disulfonic acid sodium salt) were synthesized, and their chemical formulas were finalized as [Zn(L1)Cl2]·5H2O·ZnCl2 (1), [Zn(L1)2Cl2]·4H2O·2CH3OH (2), [Zn(L2)Cl2]·3H2O·CH3OH (3), and [Zn(L2)2Cl2] (4). The synthesized complexes are water soluble, making them good candidates for biological applications. All four complexes have been characterized by elemental analysis and 1H NMR, IR, and UV-Vis spectroscopy. The IR stretching frequency of N=N and C=N bonds of complexes 1–4 have shifted to lower frequencies in comparison with free ligands, and a bathochromic shift was observed in UV-Vis spectra of all four complexes. The binding studies of ligands and complexes 1–4 with bovine serum albumin (BSA) resulted binding constants (K b) of 3.09 × 104 M−1, 12.30 × 104 M−1, and 16.84 × 104 M−1 for ferene, complex 1, and complex 2, respectively, indicating potent serum distribution via albumins. PMID:29670646

  14. Specific Detection of Enteroaggregative Hemorrhagic Escherichia coli O104:H4 Strains by Use of the CRISPR Locus as a Target for a Diagnostic Real-Time PCR

    PubMed Central

    Delannoy, Sabine; Beutin, Lothar; Burgos, Ylanna

    2012-01-01

    In 2011, a large outbreak of an unusual bacterial strain occurred in Europe. This strain was characterized as a hybrid of an enteroaggregative Escherichia coli (EAEC) and a Shiga toxin-producing E. coli (STEC) strain of the serotype O104:H4. Here, we present a single PCR targeting the clustered regularly interspaced short palindromic repeats locus of E. coli O104:H4 (CRISPRO104:H4) for specific detection of EAEC STEC O104:H4 strains from different geographical locations and time periods. The specificity of the CRISPRO104:H4 PCR was investigated using 1,321 E. coli strains, including reference strains for E. coli O serogroups O1 to O186 and flagellar (H) types H1 to H56. The assay was compared for specificity using PCR assays targeting different O104 antigen-encoding genes (wbwCO104, wzxO104, and wzyO104). The PCR assays reacted with all types of E. coli O104 strains (O104:H2, O104:H4, O104:H7, and O104:H21) and with E. coli O8 and O9 strains carrying the K9 capsular antigen and were therefore not specific for detection of the EAEC STEC O104:H4 type. A single PCR developed for the CRISPRO104:H4 target was sufficient for specific identification and detection of the 48 tested EAEC STEC O104:H4 strains. The 35 E. coli O104 strains expressing H types other than H4 as well as 8 E. coli strains carrying a K9 capsular antigen tested all negative for the CRISPRO104:H4 locus. Only 12 (0.94%) of the 1,273 non-O104:H4 E. coli strains (serotypes Ont:H2, O43:H2, O141:H2, and O174:H2) reacted positive in the CRISPRO104:H4 PCR (99.06% specificity). PMID:22895033

  15. Characterization of endogenous nanoparticles from roasted chicken breasts.

    PubMed

    Song, Xunyu; Cao, Lin; Cong, Shuang; Song, Yukun; Tan, Mingqian

    2018-06-22

    Emergence of endogenous nanoparticles in thermally processed food has aroused much attention due to their unique properties and potential biological impact. The aim of this study was to investigate the presence of fluorescence nanoparticles in roasted chicken breasts, elemental composition, physico-chemical properties and their molecular interaction with human serum albumin (HSA). Transmission electron microscopy analysis revealed that the foodborne nanoparticles from roasted chicken were nearly spherical with an average particle size of 1.7 ± 0.4 nm. The elemental analysis of X-ray photoelectron spectroscopy showed the composition of nanoparticles as 47.4% C, 25.8% O and 26.1% N. The fluorescence of HSA was quenched by the nanoparticles following a static mode, and the molecular interaction of nanoparticles with HSA was spontaneous (ΔG°＜0), where hydrogen bonding and van der Waals forces played an important role during HSA-nanoparticles complex stabilization through thermodynamic analysis by isothermal titration calorimetry. The principal location of the nanoparticles binding site on HSA was primarily in site I as determined by site-specific marker competition. The conformational of HSA was also changed and ɑ-helical structure decreased in the presence of nanoparticles. Our studies revealed that fluorescent nanoparticles were produced after roasting of chicken breast at 230 °C for 30 min for the first time. The obtained nanoparticles can interact with HSA in a spontaneous manner, thus providing valuable insight into foodborne NPs as well as their effects to human albumin protein.

  16. L x-ray production cross sections in Th and U at 17.8, 25.8 and 46.9 keV photon energies

    NASA Astrophysics Data System (ADS)

    Kumar, Ajay; Puri, Sanjiv; Shahi, J. S.; Garg, M. L.; Mehta, D.; Singh, Nirmal

    2001-02-01

    The L x-ray production (XRP) differential cross sections in Th and U have been measured at the 17.8 keV incident photon energy (E_L3

  17. Simultaneous Evaporation of Cu and Sn from Liquid Steel

    NASA Astrophysics Data System (ADS)

    Jung, Sung-Hoon; Kang, Youn-Bae

    2016-08-01

    In order to understand evaporation refining of tramp elements in molten ferrous scrap, Cu and Sn, a series of experiments were carried out using liquid-gas reaction in a levitation melting equipment. Effect of S and C, which are abundant in hot metal from ironmaking process, was examined and analyzed by employing a comprehensive evaporation kinetic model developed by the present authors (Jung et al. in Metall Mater Trans B 46B:250-258, 2014; Jung et al. in Metall Mater Trans B 46B:259-266, 2014; Jung et al. in Metall Mater Trans B 46B:267-277, 2014; Jung and Kang in Metall Mater Trans B 10.1007/s11663-016-0601-5, 2016). Evaporation of Cu and Sn were treated by evaporation of individual species such as Cu(g), CuS(g), Sn(g), and SnS(g), along with CS2(g). Decrease of Cu and Sn content in liquid steel was in good agreement with the model prediction. Optimum conditions of steel composition for the rapid evaporation of Cu and Sn were proposed by utilizing the model predictions.

  18. Sensitivity Monitoring of the SECCHI COR1 Telescopes on STEREO

    NASA Astrophysics Data System (ADS)

    Thompson, William T.

    2018-03-01

    Measurements of bright stars passing through the fields of view of the inner coronagraphs (COR1) on board the Solar Terrestrial Relations Observatory (STEREO) are used to monitor changes in the radiometric calibration over the course of the mission. Annual decline rates are found to be 0.648 ± 0.066%/year for COR1-A on STEREO Ahead and 0.258 ± 0.060%/year for COR1-B on STEREO Behind. These rates are consistent with decline rates found for other space-based coronagraphs in similar radiation environments. The theorized cause for the decline in sensitivity is darkening of the lenses and other optical elements due to exposure to high-energy solar particles and photons, although other causes are also possible. The total decline in the COR-B sensitivity when contact with Behind was lost on 1 October 2014 was 1.7%, while COR1-A was down by 4.4%. As of 1 November 2017, the COR1-A decline is estimated to be 6.4%. The SECCHI calibration routines will be updated to take these COR1 decline rates into account.

  19. Processing and Testing of Thermoplastic Composite Cylindrical Shells Fabricated by Automated Fiber Placement

    NASA Technical Reports Server (NTRS)

    Hulcher, Anthony Bruce; McGowan, David M.; Grimsley, Brian W.; Johnston, Norman J.; Gordon, Gail H. (Technical Monitor)

    2001-01-01

    Two 61-cm-diameter eight-ply quasi-isotropic IM7/PEEK cylindrical shells were fabricated by automated fiber placement the NASA Langley Research Center using only infrared radiant heat to preheat the substrate and incoming composite uni-tape. The shells were characterized by ultrasonic c-scans for overall consolidation quality, and by optical microscopy and acid digestion for void content. Compression tests were also performed. Although the material used in the study was of generally poor quality due to numerous splits and dry fiber regions, the process was able to achieve a net reduction in void content in the as-placed component. Microscopy of the composite shells revealed well-consolidated, void-free interfaces. The two cylinders were then tested in uni-axial compression in a 1334 kN-capacity hydraulic test machine until buckling occurred. A geometrically nonlinear finite element analysis was conducted, and the differences between the predicted and measured values were 18.0 and 25.8%, respectively. Inclusion of measured imperfections of the cylinder into the analysis is expected to reduce these differences.

  20. 40 CFR 258.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...; fertilizer/agricultural chemicals; food and related products/by-products; inorganic chemicals; iron and steel manufacturing; leather and leather products; nonferrous metals manufacturing/foundries; organic chemicals...

  1. A preoperative low cancer antigen 125 level (≤25.8 mg/dl) is a useful criterion to determine the optimal timing of interval debulking surgery following neoadjuvant chemotherapy in epithelial ovarian cancer.

    PubMed

    Morimoto, Akemi; Nagao, Shoji; Kogiku, Ai; Yamamoto, Kasumi; Miwa, Maiko; Wakahashi, Senn; Ichida, Kotaro; Sudo, Tamotsu; Yamaguchi, Satoshi; Fujiwara, Kiyoshi

    2016-06-01

    The purpose of this study is to investigate the clinical characteristics to determine the optimal timing of interval debulking surgery following neoadjuvant chemotherapy in patients with advanced epithelial ovarian cancer. We reviewed the charts of women with advanced epithelial ovarian cancer, fallopian tube cancer or primary peritoneal cancer who underwent interval debulking surgery following neoadjuvant chemotherapy at our cancer center from April 2006 to April 2014. There were 139 patients, including 91 with ovarian cancer [International Federation of Gynecology and Obstetrics (FIGO) Stage IIIc in 56 and IV in 35], two with fallopian tube cancers (FIGO Stage IV, both) and 46 with primary peritoneal cancer (FIGO Stage IIIc in 27 and IV in 19). After 3-6 cycles (median, 4 cycles) of platinum-based chemotherapy, interval debulking surgery was performed. Sixty-seven patients (48.2%) achieved complete resection of all macroscopic disease, while 72 did not. More patients with cancer antigen 125 levels ≤25.8 mg/dl at pre-interval debulking surgery achieved complete resection than those with higher cancer antigen 125 levels (84.7 vs. 21.3%; P< 0.0001). Patients with no ascites at pre-interval debulking surgery also achieved a higher complete resection rate (63.5 vs. 34.1%; P< 0.0001). Moreover, most patients (86.7%) with cancer antigen 125 levels ≤25.8 mg/dl and no ascites at pre-interval debulking surgery achieved complete resection. A low cancer antigen 125 level of ≤25.8 mg/dl and the absence of ascites at pre-interval debulking surgery are major predictive factors for complete resection during interval debulking surgery and present useful criteria to determine the optimal timing of interval debulking surgery. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  2. Report: landfill alternative daily cover: conserving air space and reducing landfill operating cost.

    PubMed

    Haughey, R D

    2001-02-01

    Title 40, Part 258 of the Code of Federal Regulations, Solid Waste Disposal Facility Criteria, commonly referred to as Subtitle D, became effective on October 9, 1993. It establishes minimum criteria for solid waste disposal facility siting, design, operations, groundwater monitoring and corrective action, and closure and postclosure maintenance, while providing EPA-approved state solid waste regulatory programs flexibility in implementing the criteria. Section 258.21(a) [40 CFR 258.21(a)] requires owners or operators of municipal solid waste landfill (MSWLF) units to cover disposed solid waste with 30cm of earthen material at the end of the operating day, or at more frequent intervals, if necessary, to control disease vectors, fires, odours, blowing litter, and scavenging. This requirement is consistent with already existing solid waste facility regulations in many states. For many MSWLFs, applying daily cover requires the importation of soil which increases landfill operating costs. Daily cover also uses valuable landfill air space, reducing potential operating revenue and the landfill's operating life. 40 CFR 258.21 (b) allows the director of an approved state to approve alternative materials of an alternative thickness if the owner or operator demonstrates that the alternative material and thickness will control disease vectors, fires, odours, blowing litter, and scavenging without presenting a threat to human health and the environment. Many different types of alternative daily cover (ADC) are currently being used, including geosynthetic tarps, foams, garden waste, and auto shredder fluff. These materials use less air space than soil and can reduce operating costs. This paper discusses the variety of ADCs currently being used around the country and their applicability to different climates and operating conditions, highlighting the more unusual types of ADC, the types of demonstrations necessary to obtain approval of ADC, and the impact on landfill air space and operating costs of ADC use.

  3. Studies of Flerovium and Element 115 Homologs with Macrocyclic Extractants

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Despotopulos, John D.

    2015-03-12

    Study of the chemistry of the heaviest elements, Z ≥ 104, poses a unique challenge due to their low production cross-sections and short half-lives. Chemistry also must be studied on the one-atom-at-a-time scale, requiring automated, fast, and very efficient chemical schemes. Recent studies of the chemical behavior of copernicium (Cn, element 112) and flerovium (Fl, element 114) together with the discovery of isotopes of these elements with half-lives suitable for chemical studies have spurred a renewed interest in the development of rapid systems designed to study the chemical properties of elements with Z ≥ 114. This dissertation explores both extractionmore » chromatography and solvent extraction as methods for development of a rapid chemical separation scheme for the homologs of flerovium (Pb, Sn, Hg) and element 115 (Bi, Sb), with the goal of developing a chemical scheme that, in the future, can be applied to on-line chemistry of both Fl and element 115. Carrier-free radionuclides, used in these studies, of the homologs of Fl and element 115 were obtained by proton activation of high-purity metal foils at the Lawrence Livermore National Laboratory (LLNL) Center for Accelerator Mass Spectrometry (CAMS): natIn(p,n) 113Sn, natSn(p,n) 124Sb, and Au(p,n) 197m,gHg. The carrier-free activity was separated from the foils by novel separation schemes based on ion exchange and extraction chromatography techniques. Carrier-free Pb and Bi isotopes were obtained from development of a novel generator based on cation exchange chromatography using the 232U parent to generate 212Pb and 212Bi. Macrocyclic extractants, specifically crown ethers and their derivatives, were chosen for these studies; crown ethers show high selectivity for metal ions. Finally. a potential chemical system for Fl was established based on the Eichrom Pb resin, and insight to an improved system based on thiacrown ethers is presented.« less

  4. Geochemical fractions of rare earth elements in soil around a mine tailing in Baotou, China

    PubMed Central

    Wang, Lingqing; Liang, Tao

    2015-01-01

    Rare earth mine tailing dumps are environmental hazards because tailing easily leaches and erodes by water and wind. To assess the influence of mine tailing on the geochemical behavior of rare earth elements (REEs) in soil, sixty-seven surface soil samples and three soil profile samples were collected from different locations near China’s largest rare earth mine tailing. The total concentration of REEs in surface soils ranged from 156 to 5.65 × 104 mg·kg−1 with an average value of 4.67 × 103 mg·kg−1, which was significantly higher than the average value in China (181 mg·kg−1). We found obvious fractionation of both light and heavy REEs, which was supported by the North American Shale Composite (NASC) and the Post-Archean Average Australian Shale (PAAS) normalized concentration ratios calculated for selected elements (LaN/YbN, LaN/SmN and GdN/YbN). A slightly positive Ce anomaly and a negative Eu anomaly were also found. For all 14 REEs in soils, enrichment was intensified by the mine tailing sources and influenced by the prevailing wind. PMID:26198417

  5. Flipping chromosomes in deep-sea archaea

    PubMed Central

    Catchpole, Ryan; Gadelle, Danièle; Marguet, Evelyne; Barbe, Valérie; Forterre, Patrick

    2017-01-01

    One of the major mechanisms driving the evolution of all organisms is genomic rearrangement. In hyperthermophilic Archaea of the order Thermococcales, large chromosomal inversions occur so frequently that even closely related genomes are difficult to align. Clearly not resulting from the native homologous recombination machinery, the causative agent of these inversions has remained elusive. We present a model in which genomic inversions are catalyzed by the integrase enzyme encoded by a family of mobile genetic elements. We characterized the integrase from Thermococcus nautili plasmid pTN3 and showed that besides canonical site-specific reactions, it catalyzes low sequence specificity recombination reactions with the same outcome as homologous recombination events on DNA segments as short as 104bp both in vitro and in vivo, in contrast to other known tyrosine recombinases. Through serial culturing, we showed that the integrase-mediated divergence of T. nautili strains occurs at an astonishing rate, with at least four large-scale genomic inversions appearing within 60 generations. Our results and the ubiquitous distribution of pTN3-like integrated elements suggest that a major mechanism of evolution of an entire order of Archaea results from the activity of a selfish mobile genetic element. PMID:28628615

  6. Geochemical fractions of rare earth elements in soil around a mine tailing in Baotou, China.

    PubMed

    Wang, Lingqing; Liang, Tao

    2015-07-22

    Rare earth mine tailing dumps are environmental hazards because tailing easily leaches and erodes by water and wind. To assess the influence of mine tailing on the geochemical behavior of rare earth elements (REEs) in soil, sixty-seven surface soil samples and three soil profile samples were collected from different locations near China's largest rare earth mine tailing. The total concentration of REEs in surface soils ranged from 156 to 5.65 × 10(4) mg·kg(-1) with an average value of 4.67 × 10(3) mg·kg(-1), which was significantly higher than the average value in China (181 mg·kg(-1)). We found obvious fractionation of both light and heavy REEs, which was supported by the North American Shale Composite (NASC) and the Post-Archean Average Australian Shale (PAAS) normalized concentration ratios calculated for selected elements (La(N)/Yb(N), La(N)/Sm(N) and Gd(N)/Yb(N)). A slightly positive Ce anomaly and a negative Eu anomaly were also found. For all 14 REEs in soils, enrichment was intensified by the mine tailing sources and influenced by the prevailing wind.

  7. The effect of smoking on periodontal health of 15- to 16-year-old adolescents.

    PubMed

    Heikkinen, Anna Maria; Pajukanta, Riitta; Pitkäniemi, Janne; Broms, Ulla; Sorsa, Timo; Koskenvuo, Markku; Meurman, Jukka H

    2008-11-01

    Smoking is a severe risk factor for periodontal health in adults, but data on the effect of smoking on periodontal health in teenage populations are sparse. The aim of this study was to investigate the effect of duration and quantity of smoking on periodontal health in teenagers and possible differences between genders. The oral health of 501 adolescents (15- to 16-year-old boys [n = 258] and girls [n = 243]) was examined. A structured questionnaire about self-reported smoking and health habits was filled out, and bitewing x-rays were taken. Clinical examinations included measuring periodontal indexes, such as visible plaque index, bleeding on probing, root calculus (RC), probing depth, and attachment loss. Results were analyzed by generalized linear logistic regression. Twenty-five percent of boys and 27% of girls were smokers. The boys and girls who smoked had higher RC values than non-smokers (P <0.001). The adjusted scores for smoking boys and girls were 17.3 (95% confidence interval [CI]: 8.6 to 31.7) and 13.6 (95% CI: 5.5 to 29.7), respectively. The adjusted scores for non-smokers were 10.4 (95% CI: 5.7 to 18.3) and 7.7 (95% CI: 3.3 to 17.3), respectively. Smoking boys and girls also had more periodontal pockets > or =4 mm than non-smokers: the score for boys was 4.6 (95% CI: 2.2 to 9.1), and the score for girls was 5.4 (95% CI: 1.1 to 23.2; P <0.001). Smoking significantly impaired periodontal health in teenagers.

  8. Transcriptome analysis of mud crab (Scylla paramamosain) gills in response to Mud crab reovirus (MCRV).

    PubMed

    Liu, Shanshan; Chen, Guanxing; Xu, Haidong; Zou, Weibin; Yan, Wenrui; Wang, Qianqian; Deng, Hengwei; Zhang, Heqian; Yu, Guojiao; He, Jianguo; Weng, Shaoping

    2017-01-01

    Mud crab (Scylla paramamosain) is an economically important marine cultured species in China's coastal area. Mud crab reovirus (MCRV) is the most important pathogen of mud crab, resulting in large economic losses in crab farming. In this paper, next-generation sequencing technology and bioinformatics analysis are used to study transcriptome differences between MCRV-infected mud crab and normal control. A total of 104.3 million clean reads were obtained, including 52.7 million and 51.6 million clean reads from MCRV-infected (CA) and controlled (HA) mud crabs respectively. 81,901, 70,059 and 67,279 unigenes were gained respectively from HA reads, CA reads and HA&CA reads. A total of 32,547 unigenes from HA&CA reads called All-Unigenes were matched to at least one database among Nr, Nt, Swiss-prot, COG, GO and KEGG databases. Among these, 13,039, 20,260 and 11,866 unigenes belonged to the 3, 258 and 25 categories of GO, KEGG pathway, and COG databases, respectively. Solexa/Illumina's DGE platform was also used, and about 13,856 differentially expressed genes (DEGs), including 4444 significantly upregulated and 9412 downregulated DEGs were detected in diseased crabs compared with the control. KEGG pathway analysis revealed that DEGs were obviously enriched in the pathways related to different diseases or infections. This transcriptome analysis provided valuable information on gene functions associated with the response to MCRV in mud crab, as well as detail information for identifying novel genes in the absence of the mud crab genome database. Copyright © 2016. Published by Elsevier Ltd.

  9. Kerteszia Theobald (Diptera: Culicidae) mosquitoes and bromeliads: A landscape ecology approach regarding two species in the Atlantic rainforest.

    PubMed

    Chaves, Leonardo Suveges Moreira; Rodrigues de Sá, Ivy Luizi; Bergamaschi, Denise Pimentel; Sallum, Maria Anice Mureb

    2016-12-01

    On the ecological scale of an organism, a homogeneous geographical landscape can represent a mosaic of heterogeneous landscapes. The bionomy of Kerteszia mosquitoes can contribute to foundation landscape ecology by virtue of in the role of the configuration and composition of the habitat played in the distribution of mosquito species. Thus, this study aimed: to compare the abundance of Kerteszia in dense tropical rainforest, restinga and rural area, to assess the bioecological characteristics of the main bromeliads hosting Kerteszia, and to associate the bioecological arrangement of the bromeliads with Kerteszia distribution. Field collections were conducted in a monthly schedule from December of 2010 to November 2011. The vegetation of landscapes was characterized on the basis of a digital cartographic database, the manual of the Brazilian vegetation, environmental atlas information, satellite images and visits to the sites. Multivariate generalized linear models were employed using the R-project statistical program. The results were: Anopheles cruzii was the most frequent species in dense tropical rainforest (67.42%), with a positive association (deviance=25.8; P=0.002). Anopheles bellator was more abundant in the Restinga area (78.97%), with a positive association (deviance=10.4, P=0.018). There was a positive aggregation of Restinga with An. bellator (RR=2.42) but a lower level with An. cruzii (RR=0.31). Thus we can conclude that landscape characteristics influence the distribution of Kerteszia mosquitoes. An. bellator has a higher prevalence in Restinga areas, whereas An. cruzii was the most prevalent in the dense tropical rainforest. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. Chemical Composition and Biological Studies of the Essential Oil from Aerial Parts of Beta vulgaris subsp. maritima (L.) Arcang. Growing in Tunisia.

    PubMed

    Zardi-Bergaoui, Afifa; Ben Nejma, Aymen; Harzallah-Skhiri, Fethia; Flamini, Guido; Ascrizzi, Roberta; Ben Jannet, Hichem

    2017-10-01

    The chemical composition, antioxidant, cytotoxic, anticholinesterase and anti-tyrosinase activities of the hydrodistilled essential oil of the aerial parts of Beta vulgaris subsp. maritime (L.) Arcang. from Tunisia have been evaluated. The chemical composition of the oil (yield 0.037% [w/w]), determined by GC-FID and GC/MS is reported for the first time. Twenty five components, accounting for 98.1% of the total oil have been identified. The oil was characterized by a high proportion of oxygenated sesquiterpenes (39.2%), followed by sesquiterpene hydrocarbons (30.3%) and one apocarotenoids (26.3%). The main compounds were γ-irone (26.3%), α-cadinol (12.1%), T-cadinol (10.6%), bicyclogermacrene (10.4%) and δ-cadinene (6.0%). The isolated oil was tested for its antioxidant activity using the DPPH · , ABTS +· , catalase, and paraoxonase assays and also for its cytotoxic, anticholinesterase, and anti-tyrosinase activities. The essential oil exhibited high antioxidant activity (IC 50  = 0.055 ± 0.006 mg/ml) and important result oncatalase (524.447 ± 2.58 Units/mg protein). Furthermore, it exerted a significant cytotoxic effect against A549 cell line, with IC 50  = 42.44 ± 1.40 μg/ml. The results indicate that the essential oil of B. vulgaris subsp. maritima (L.) Arcang. aerial parts may be used in future as an alternative to synthetic antioxidant agents, with potential application in the food and pharmaceutical industries. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  11. Somatic mutation frequencies in the stamen hairs of Tradescantia grown in soil samples from the Bikini Island.

    PubMed

    Ichikawa, S; Ishii, C

    1991-02-01

    Somatic pink mutation frequencies in the stamen hairs of Tradescantia BNL 02 clone grown for 76 days in two soil samples taken from the Bikini Island (where a hydrogen bomb explosion test had been conducted in 1954) were investigated. A significantly high mutation frequency (2.58 +/- 0.17 pink mutant events per 10(3) hairs or 1.34 +/- 0.09 pink mutant events per 10(4) hair-cell divisions) was observed for the plant grown in one of the two Bikini soil samples, as compared to the control plants (1.70 +/- 0.14 or 0.88 +/- 0.07, respectively) grown in the field soil of Saitama University. The soil sample which caused the significant increase in mutation frequency contained 6,880 +/- 330 mBq/g 137Cs, 62.5 +/- 4.4 mBq/g 60Co, and some other nuclides; a 150 microR/hr exposure rate being measured on the surface of the soil sample. The effective cumulative external exposures measured for the inflorescences of the plant grown in this soil sample averaged at most 60.8 mR, being too small to explain the significant elevation in mutation frequency observed. On the other hand, internal exposure due to uptake of radioactive nuclides was estimated to be 125 mrad (1.25 mGy) as an accumulated effective dose, mainly based on a gamma-spectrometrical analysis. However, it seemed highly likely that this value of internal exposure was a considerable underestimate, and the internal exposure was considered to be more significant than the external exposure.

  12. Orthorexic and restrained eating behaviour in vegans, vegetarians, and individuals on a diet.

    PubMed

    Barthels, Friederike; Meyer, Frank; Pietrowsky, Reinhard

    2018-04-01

    Orthorexic eating behaviour, restrained eating, and veganism/vegetarianism are food selection strategies sharing several characteristics. Since there are no studies investigating their interrelationships, aim of the present study was to analyse orthorexic and restrained eating behaviour in (1) a sample of vegans and vegetarians and (2) a sample of individuals on a diet to lose weight. Division of samples according to pre-defined criteria in (1) vegans (n = 114), vegetarians (n = 63), individuals with rare meat consumption (n = 83) and individuals with frequent meat consumption (n = 91) and in (2) participants on a diet with dietary change (n = 104), without dietary change (n = 37) and a control group of individuals not on a diet (n = 258). Orthorexic eating behaviour was assessed with the Düsseldorfer Orthorexie Skala and restrained eating was assessed with the Restraint Eating Scale. Vegans and vegetarians do not differ in orthorexic eating behaviour, but both groups score higher in orthorexic eating behaviour than individuals consuming red meat. There are no differences regarding restrained eating. Individuals on a diet with dietary change score higher in both orthorexic and restrained eating, than individuals without dietary change and individuals not on a diet. Individuals who restrict their eating behaviour, either predominantly due to ethical reasons or with the intention to lose weight, display more orthorexic eating behaviour than individuals not limiting their food consumption. Further research is needed to investigate whether veganism, vegetarianism, or frequent dieting behaviour serve as risk factors for orthorexia. Level V, cross-sectional descriptive study.

  13. Modeling of optical mirror and electromechanical behavior

    NASA Astrophysics Data System (ADS)

    Wang, Fang; Lu, Chao; Liu, Zishun; Liu, Ai Q.; Zhang, Xu M.

    2001-10-01

    This paper presents finite element (FE) simulation and theoretical analysis of novel MEMS fiber-optical switches actuated by electrostatic attraction. FE simulation for the switches under static and dynamic loading are first carried out to reveal the mechanical characteristics of the minimum or critical switching voltages, the natural frequencies, mode shapes and response under different levels of electrostatic attraction load. To validate the FE simulation results, a theoretical (or analytical) model is then developed for one specific switch, i.e., Plate_40_104. Good agreement is found between the FE simulation and the analytical results. From both FE simulation and theoretical analysis, the critical switching voltage for Plate_40_104 is derived to be 238 V for the switching angel of 12 degree(s). The critical switching on and off times are 431 microsecond(s) and 67 microsecond(s) , respectively. The present study not only develops good FE and analytical models, but also demonstrates step by step a method to simplify a real optical switch structure with reference to the FE simulation results for analytical purpose. With the FE and analytical models, it is easy to obtain any information about the mechanical behaviors of the optical switches, which are helpful in yielding optimized design.

  14. Growth of Cu2ZnSnS4(CZTS) by Pulsed Laser Deposition for Thin film Photovoltaic Absorber Material

    NASA Astrophysics Data System (ADS)

    Nandur, Abhishek; White, Bruce

    2014-03-01

    CZTS (Cu2ZnSnS4) has become the subject of intense interest because it is an ideal candidate absorber material for thin-film solar cells with an optimal band gap (1.5 eV), high absorption coefficient (104 cm-1) and abundant elemental components. Pulsed Laser Deposition (PLD) provides excellent control over film composition since thin films are deposited under high vacuum with excellent stoichiometry transfer from the target. CZTS thin films were deposited using PLD from a stoichiometrically close CZTS target (Cu2.6Zn1.1Sn0.7S3.44). The effects of laser energy fluence and substrate temperature and post-deposition sulfur annealing on the surface morphology, composition and optical absorption have been investigated. Optimal CZTS thin films exhibited a band gap of 1.54 eV with an absorption coefficient of 4x104cm-1. A solar cell utilizing PLD grown CZTS with the structure SLG/Mo/CZTS/CdS/ZnO/ITO showed a conversion efficiency of 5.85% with Voc = 376 mV, Jsc = 38.9 mA/cm2 and Fill Factor, FF = 0.40.

  15. τ → f1(1285) π-ν_{τ} decay in the extended Nambu-Jona-Lasinio model

    NASA Astrophysics Data System (ADS)

    Volkov, M. K.; Pivovarov, A. A.; Osipov, A. A.

    2018-04-01

    Within the framework of the extended Nambu-Jona-Lasinio model, we calculate the matrix element of the τ → f1(1285) π- ν_{τ} decay, obtain the invariant mass distribution of the f1π-system and estimate the branching ratio Br(τ → f1 π-ν_{τ}) = 4.0× 10^{-4}. The two types of contributions are considered: the contact interaction, and the axial-vector IG(J^{PC})=1-(1^{++}) resonance exchange. The latter includes the ground a1(1260) state, and its first radially excited state, a1(1640). The corrections caused by the π-a1 transitions are taken into account. Our estimate is in a good agreement with the latest empirical result Br(τ → f1 π- ν_{τ})=(3.9± 0.5)× 10^{-4}. The distribution function obtained for the decay τ → f1(1285) π- ν_{τ} shows a clear signal of a1(1640) resonance which should be compared with future experimental data including our estimate of the decay width Γ (a1(1640) → f1 π)=14.1 MeV.

  16. Functionalisation of mesoporous silica gel with 2-[(phosphonomethyl)-amino]acetic acid functional groups. Characterisation and application

    NASA Astrophysics Data System (ADS)

    Caldarola, Dario; Mitev, Dimitar P.; Marlin, Lucile; Nesterenko, Ekaterina P.; Paull, Brett; Onida, Barbara; Bruzzoniti, Maria Concetta; Carlo, Rosa Maria De; Sarzanini, Corrado; Nesterenko, Pavel N.

    2014-01-01

    A new complexing adsorbent was prepared by chemical modification of mesoporous silica Kieselgel 60 (dp = 37-63 μm, average pore size 6 nm, specific surface area 425 m2 g-1) with 3-glycidoxypropyltrimethoxysilane and 2-[(phosphonomethyl)amino]acetic acid (PMA), commonly known as glyphosate. The prepared adsorbent was fully characterised using elemental analysis, thermal gravimetric analysis (TGA), acid-base potentiometric titration, Fourier Transform Infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), nitrogen adsorption isotherms at 77 K (BET), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDS). The concentration of bonded PMA groups calculated from the nitrogen content was 0.38 mmol per gram. The adsorption of transition metal ions on PMA functionalised silica (HEPMAS) was studied from aqueous solutions having different pH and the following selectivity was established, Zn(II) < Co(II) < Cd(II) < Mn(II) < Ni(II) < Cu(II). The calculated values of distribution coefficients D for the adsorption of ecotoxic metal ions on HEPMAS are 5.0 × 104, 4.9 × 105 and 2.6 × 104 for Zn(II), Pb(II) and Cd(II), respectively.

  17. Preliminary X-Ray Crystallographic Studies of the N-Terminal Domains of Hsp104 from Yeast Candida albicans and Saccharomyces cerevisiae

    NASA Astrophysics Data System (ADS)

    Wang, P.; Li, J.; Sha, B.

    2017-12-01

    Yeast Hsp104 is an ATP-dependent molecular chaperone, which can solublize and rescue denatured proteins from aggregates into active form by cooperating with Hsp70 and Hsp40 chaperones. Moreover, overexpression of Hsp104 of Saccharomyces cerevisiae (ScHsp104) cures the yeast [ PSI +] prion due to the completely dissolution of the prion seeds, demonstrating ScHsp104's potential to clear amyloid-like protein aggregates, thus making ScHsp104 a promising medication approach for human amyloidogenic neurodegenerative diseases. Because the working mechanisms for ScHsp104's activities have not been clearly elucidated yet, crystallographic determination of ScHsp104 stands for great significance. Here, the expression, purification and crystallization of the N-terminal domains of Hsp104 from yeast Candida albicans (CaHsp104N) and S. cerevisiae (ScHsp104N) are described. The CaHsp104N crystals diffracted to 1.54 Å and belonged to the sp. gr. P3221 or P3121, with unit cell parameters of a = 55.213 Å, c = 109.451 Å. The data of the ScHsp104N crystals were collected to the resolution of 2.53 Å in the sp. gr. C2, with unit cell parameters a = 148.587 Å, b = 66.255 Å, c = 74.577 Å, β = 107.369°. The phase of ScHsp104N is determined by the molecular replacement method using CaHsp104N as the search model.

  18. Reconfigurable ultra-wideband waveform generation with simple photonic devices

    NASA Astrophysics Data System (ADS)

    Dastmalchi, Mansour; Abtahi, Mohammad; Lemus, David; Rusch, Leslie A.; LaRochelle, Sophie

    2012-08-01

    We propose and experimentally demonstrate a low cost, low power consumption technique for ultra-wideband pulse shaping. Our approach is based on thermal apodization of two identical linearly chirped fiber Bragg gratings (LCFBG) placed in both arms of a balanced photodetector. Resistive heating elements with low electrical power consumption are used to tune the LCFBG spectral responses. Using a standard gain switched distributed feedback laser as a pulsed optical source and a simple energy detector receiver, we measured a bit error rate of 1.5×10-4 at a data rate of 1 Gb/s after RF transmission over a 1-m link.

  19. Reliability assessment of MVP-BURN and JENDL-4.0 related to nuclear transmutation of light platinum group elements

    NASA Astrophysics Data System (ADS)

    Terashima, Atsunori; Nilsson, Mikael; Ozawa, Masaki; Chiba, Satoshi

    2017-09-01

    The Aprés ORIENT research program, as a concept of advanced nuclear fuel cycle, was initiated in FY2011 aiming at creating stable, highly-valuable elements by nuclear transmutation from ↓ssion products. In order to simulate creation of such elements by (n, γ) reaction succeeded by β- decay in reactors, a continuous-energy Monte Carlo burnup calculation code MVP-BURN was employed. Then, it is one of the most important tasks to con↓rm the reliability of MVP-BURN code and evaluated neutron cross section library. In this study, both an experiment of neutron activation analysis in TRIGA Mark I reactor at University of California, Irvine and the corresponding burnup calculation using MVP-BURN code were performed for validation of the simulation on transmutation of light platinum group elements. Especially, some neutron capture reactions such as 102Ru(n, γ)103Ru, 104Ru(n, γ)105Ru, and 108Pd(n, γ)109Pd were dealt with in this study. From a comparison between the calculation (C) and the experiment (E) about 102Ru(n, γ)103Ru, the deviation (C/E-1) was signi↓cantly large. Then, it is strongly suspected that not MVP-BURN code but the neutron capture cross section of 102Ru belonging to JENDL-4.0 used in this simulation have made the big di↑erence as (C/E-1) >20%.

  20. Genotypes and virulence characteristics of Shiga toxin-producing Escherichia coli O104 strains from different origins and sources.

    PubMed

    Miko, Angelika; Delannoy, Sabine; Fach, Patrick; Strockbine, Nancy A; Lindstedt, Björn Arne; Mariani-Kurkdjian, Patricia; Reetz, Jochen; Beutin, Lothar

    2013-12-01

    Sixty-two Escherichia coli strains carrying the wzxO104-gene from different sources, origins and time periods were analyzed for their serotypes, virulence genes and compared for genomic similarity by pulsed-field gel-electrophoresis (PFGE). The O104 antigen was present in 55 strains and the structurally and genetically related capsular antigen K9 in five strains. The presence of 49 genes associated with enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC) and enterohemorrhagic E. coli (EHEC) was investigated. Fifty-four strains of serotypes O104:H2 (n=1), O104:H4 (n=37), O104:H7 (n=5) and O104:H21 (n=11) produced Shiga-toxins (Stx). Among STEC O104, a close association between serotype, virulence gene profile and genomic similarity was found. EAEC virulence genes were only present in STEC O104:H4 strains. EHEC-O157 plasmid-encoded genes were only found in STEC O104:H2, O104:H7 and O104:H21 strains. None of the 62 O104 or K9 strains carried an eae-gene involved in the attaching and effacing phenotype. The 38 O104:H4 strains formed a single PFGE-cluster (>83.7% similarity). Thirty-one of these strains were from the European O104:H4 outbreak in 2011. The outbreak strains and older O104:H4 strains from Germany (2001), Georgia and France (2009) clustered together at>86.2% similarity. O104:H4 strains isolated between 2001 and 2009 differed for some plasmid-encoded virulence genes compared to the outbreak strains from 2011. STEC O104:H21 and STEC O104:H7 strains isolated in the U.S. and in Europe showed characteristic differences in their Stx-types, virulence gene and PFGE profiles indicating that these have evolved separately. E. coli K9 strains were not associated with virulence and were heterogeneous for their serotypes and PFGE profiles. Copyright © 2013 Elsevier GmbH. All rights reserved.

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