The Soil Moisture Active Passive (SMAP): Radar Measurements at High Latitudes and of Freeze/Thaw State

NASA Technical Reports Server (NTRS)

Spencer, Michael; Dunbar, Scott; Chen, Curtis

2013-01-01

The Soil Moisture Active/Passive (SMAP) mission is scheduled for a late 2014 launch date. The mission will use both active radar and passive radiometer instruments at L-Band. In order to achieve a wide swath at sufficiently high resolution for both active and passive channels, an instrument architecture that uses a large rotating reflector is employed. In this paper, a focus will be places on the radar design and associated data products at high latitudes. The radar will employ synthetic-aperture processing to achieve a "moderate" resolution dual-pol product over a 1000 km swath. Because the radar is operating continuously, very frequent temporal coverage will be achieved at high latitudes. This data will be used, among other things, to produce a surface freeze/thaw state data product.

Comparison of High-Order and Low-Order Methods for Large-Eddy Simulation of a Compressible Shear Layer

NASA Technical Reports Server (NTRS)

Mankbadi, Mina R.; Georgiadis, Nicholas J.; DeBonis, James R.

2015-01-01

The objective of this work is to compare a high-order solver with a low-order solver for performing Large-Eddy Simulations (LES) of a compressible mixing layer. The high-order method is the Wave-Resolving LES (WRLES) solver employing a Dispersion Relation Preserving (DRP) scheme. The low-order solver is the Wind-US code, which employs the second-order Roe Physical scheme. Both solvers are used to perform LES of the turbulent mixing between two supersonic streams at a convective Mach number of 0.46. The high-order and low-order methods are evaluated at two different levels of grid resolution. For a fine grid resolution, the low-order method produces a very similar solution to the highorder method. At this fine resolution the effects of numerical scheme, subgrid scale modeling, and filtering were found to be negligible. Both methods predict turbulent stresses that are in reasonable agreement with experimental data. However, when the grid resolution is coarsened, the difference between the two solvers becomes apparent. The low-order method deviates from experimental results when the resolution is no longer adequate. The high-order DRP solution shows minimal grid dependence. The effects of subgrid scale modeling and spatial filtering were found to be negligible at both resolutions. For the high-order solver on the fine mesh, a parametric study of the spanwise width was conducted to determine its effect on solution accuracy. An insufficient spanwise width was found to impose an artificial spanwise mode and limit the resolved spanwise modes. We estimate that the spanwise depth needs to be 2.5 times larger than the largest coherent structures to capture the largest spanwise mode and accurately predict turbulent mixing.

Megahertz-resolution programmable microwave shaper.

PubMed

Li, Jilong; Dai, Yitang; Yin, Feifei; Li, Wei; Li, Ming; Chen, Hongwei; Xu, Kun

2018-04-15

A novel microwave shaper is proposed and demonstrated, of which the microwave spectral transfer function could be fully programmable with high resolution. We achieve this by bandwidth-compressed mapping a programmable optical wave-shaper, which has a lower frequency resolution of tens of gigahertz, to a microwave one with resolution of tens of megahertz. This is based on a novel technology of "bandwidth scaling," which employs bandwidth-stretched electronic-to-optical conversion and bandwidth-compressed optical-to-electronic conversion. We demonstrate the high resolution and full reconfigurability experimentally. Furthermore, we show the group delay variation could be greatly enlarged after mapping; this is then verified by the experiment with an enlargement of 194 times. The resolution improvement and group delay magnification significantly distinguish our proposal from previous optics-to-microwave spectrum mapping.

Single-shot and single-sensor high/super-resolution microwave imaging based on metasurface.

PubMed

Wang, Libo; Li, Lianlin; Li, Yunbo; Zhang, Hao Chi; Cui, Tie Jun

2016-06-01

Real-time high-resolution (including super-resolution) imaging with low-cost hardware is a long sought-after goal in various imaging applications. Here, we propose broadband single-shot and single-sensor high-/super-resolution imaging by using a spatio-temporal dispersive metasurface and an imaging reconstruction algorithm. The metasurface with spatio-temporal dispersive property ensures the feasibility of the single-shot and single-sensor imager for super- and high-resolution imaging, since it can convert efficiently the detailed spatial information of the probed object into one-dimensional time- or frequency-dependent signal acquired by a single sensor fixed in the far-field region. The imaging quality can be improved by applying a feature-enhanced reconstruction algorithm in post-processing, and the desired imaging resolution is related to the distance between the object and metasurface. When the object is placed in the vicinity of the metasurface, the super-resolution imaging can be realized. The proposed imaging methodology provides a unique means to perform real-time data acquisition, high-/super-resolution images without employing expensive hardware (e.g. mechanical scanner, antenna array, etc.). We expect that this methodology could make potential breakthroughs in the areas of microwave, terahertz, optical, and even ultrasound imaging.

Computer programs to assist in high resolution thermal denaturation and circular dichroism studies on nucleic acids

PubMed Central

Goodman, Thomas C.; Hardies, Stephen C.; Cortez, Carlos; Hillen, Wolfgang

1981-01-01

Computer programs are described that direct the collection, processing, and graphical display of numerical data obtained from high resolution thermal denaturation (1-3) and circular dichroism (4) studies. Besides these specific applications, the programs may also be useful, either directly or as programming models, in other types of spectrophotometric studies employing computers, programming languages, or instruments similar to those described here (see Materials and Methods). PMID:7335498

Localization-based super-resolution imaging of cellular structures.

PubMed

Kanchanawong, Pakorn; Waterman, Clare M

2013-01-01

Fluorescence microscopy allows direct visualization of fluorescently tagged proteins within cells. However, the spatial resolution of conventional fluorescence microscopes is limited by diffraction to ~250 nm, prompting the development of super-resolution microscopy which offers resolution approaching the scale of single proteins, i.e., ~20 nm. Here, we describe protocols for single molecule localization-based super-resolution imaging, using focal adhesion proteins as an example and employing either photoswitchable fluorophores or photoactivatable fluorescent proteins. These protocols should also be easily adaptable to imaging a broad array of macromolecular assemblies in cells whose components can be fluorescently tagged and assemble into high density structures.

Time-domain multiplexed high resolution fiber optics strain sensor system based on temporal response of fiber Fabry-Perot interferometers.

PubMed

Chen, Jiageng; Liu, Qingwen; He, Zuyuan

2017-09-04

We developed a multiplexed strain sensor system with high resolution using fiber Fabry-Perot interferometers (FFPI) as sensing elements. The temporal responses of the FFPIs excited by rectangular laser pulses are used to obtain the strain applied on each FFPI. The FFPIs are connected by cascaded couplers and delay fiber rolls for the time-domain multiplexing. A compact optoelectronic system performing closed-loop cyclic interrogation is employed to improve the sensing resolution and the frequency response. In the demonstration experiment, 3-channel strain sensing with resolutions better than 0.1 nε and frequency response higher than 100 Hz is realized.

A high-resolution time-to-digital converter using a three-level resolution

NASA Astrophysics Data System (ADS)

Dehghani, Asma; Saneei, Mohsen; Mahani, Ali

2016-08-01

In this article, a three-level resolution Vernier delay line time-to-digital converter (TDC) was proposed. The proposed TDC core was based on the pseudo-differential digital architecture that made it insensitive to nMOS and pMOS transistor mismatches. It also employed a Vernier delay line (VDL) in conjunction with an asynchronous read-out circuitry. The time interval resolution was equal to the difference of delay between buffers of upper and lower chains. Then, via the extra chain included in the lower delay line, resolution was controlled and power consumption was reduced. This method led to high resolution and low power consumption. The measurement results of TDC showed a resolution of 4.5 ps, 12-bit output dynamic range, and integral nonlinearity of 1.5 least significant bits. This TDC achieved the consumption of 68.43 µW from 1.1-V supply.

Collaborative classification of hyperspectral and visible images with convolutional neural network

NASA Astrophysics Data System (ADS)

Zhang, Mengmeng; Li, Wei; Du, Qian

2017-10-01

Recent advances in remote sensing technology have made multisensor data available for the same area, and it is well-known that remote sensing data processing and analysis often benefit from multisource data fusion. Specifically, low spatial resolution of hyperspectral imagery (HSI) degrades the quality of the subsequent classification task while using visible (VIS) images with high spatial resolution enables high-fidelity spatial analysis. A collaborative classification framework is proposed to fuse HSI and VIS images for finer classification. First, the convolutional neural network model is employed to extract deep spectral features for HSI classification. Second, effective binarized statistical image features are learned as contextual basis vectors for the high-resolution VIS image, followed by a classifier. The proposed approach employs diversified data in a decision fusion, leading to an integration of the rich spectral information, spatial information, and statistical representation information. In particular, the proposed approach eliminates the potential problems of the curse of dimensionality and excessive computation time. The experiments evaluated on two standard data sets demonstrate better classification performance offered by this framework.

High-resolution confocal Raman microscopy using pixel reassignment.

PubMed

Roider, Clemens; Ritsch-Marte, Monika; Jesacher, Alexander

2016-08-15

We present a practical modification of fiber-coupled confocal Raman scanning microscopes that is able to provide high confocal resolution in conjunction with high light collection efficiency. For this purpose, the single detection fiber is replaced by a hexagonal lenslet array in combination with a hexagonally packed round-to-linear multimode fiber bundle. A multiline detector is used to collect individual Raman spectra for each fiber. Data post-processing based on pixel reassignment allows one to improve the lateral resolution by up to 41% compared to a single fiber of equal light collection efficiency. We present results from an experimental implementation featuring seven collection fibers, yielding a resolution improvement of about 30%. We believe that our implementation represents an attractive upgrade for existing confocal Raman microscopes that employ multi-line detectors.

Three dimensional imaging detector employing wavelength-shifting optical fibers

DOEpatents

Worstell, William A.

1997-01-01

A novel detector element structure and method for its use is provided. In a preferred embodiment, one or more inorganic scintillating crystals are coupled through wavelength shifting optical fibers (WLSFs) to position sensitive photomultipliers (PS-PMTs). The superior detector configuration in accordance with this invention is designed for an array of applications in high spatial resolution gamma ray sensing with particular application to SPECT, PET and PVI imaging systems. The design provides better position resolution than prior art devices at a lower total cost. By employing wavelength shifting fibers (WLSFs), the sensor configuration of this invention can operate with a significant reduction in the number of photomultipliers and electronics channels, while potentially improving the resolution of the system by allowing three dimensional reconstruction of energy deposition positions.

Three dimensional imaging detector employing wavelength-shifting optical fibers

DOEpatents

Worstell, W.A.

1997-02-04

A novel detector element structure and method for its use is provided. In a preferred embodiment, one or more inorganic scintillating crystals are coupled through wavelength shifting optical fibers (WLSFs) to position sensitive photomultipliers (PS-PMTs). The superior detector configuration in accordance with this invention is designed for an array of applications in high spatial resolution gamma ray sensing with particular application to SPECT, PET and PVI imaging systems. The design provides better position resolution than prior art devices at a lower total cost. By employing wavelength shifting fibers (WLSFs), the sensor configuration of this invention can operate with a significant reduction in the number of photomultipliers and electronics channels, while potentially improving the resolution of the system by allowing three dimensional reconstruction of energy deposition positions. 11 figs.

Wide-aperture aspherical lens for high-resolution terahertz imaging

NASA Astrophysics Data System (ADS)

Chernomyrdin, Nikita V.; Frolov, Maxim E.; Lebedev, Sergey P.; Reshetov, Igor V.; Spektor, Igor E.; Tolstoguzov, Viktor L.; Karasik, Valeriy E.; Khorokhorov, Alexei M.; Koshelev, Kirill I.; Schadko, Aleksander O.; Yurchenko, Stanislav O.; Zaytsev, Kirill I.

2017-01-01

In this paper, we introduce wide-aperture aspherical lens for high-resolution terahertz (THz) imaging. The lens has been designed and analyzed by numerical methods of geometrical optics and electrodynamics. It has been made of high-density polyethylene by shaping at computer-controlled lathe and characterized using a continuous-wave THz imaging setup based on a backward-wave oscillator and Golay detector. The concept of image contrast has been implemented to estimate image quality. According to the experimental data, the lens allows resolving two points spaced at 0.95λ distance with a contrast of 15%. To highlight high resolution in the THz images, the wide-aperture lens has been employed for studying printed electronic circuit board containing sub-wavelength-scale elements. The observed results justify the high efficiency of the proposed lens design.

The Soil Moisture Active Passive (SMAP) Radar: Measurements at High Latitudes and of Surface Freeze/Thaw State

NASA Technical Reports Server (NTRS)

Spencer, Michael; Dunbar, Scott; Chen, Curtis

2013-01-01

The Soil Moisture Active/Passive (SMAP) mission is scheduled for a late 2014 launch date. The mission will use both active radar and passive radiometer instruments at L-Band in order to achieve the science objectives of measuring soil moisture and land surface freeze-thaw state. To achieve requirements for a wide swath at sufficiently high resolution for both active and passive channels, an instrument architecture that uses a large rotating reflector is employed. In this paper, focus will be placed on the radar design. The radar will employ synthetic-aperture processing to achieve a "moderate" resolution dual-pol product over a 1000 km swath. Because the radar is operating continuously, very frequent temporal coverage will be achieved at high latitudes. This data will be used to produce a surface freeze/thaw state data product.

Single-shot and single-sensor high/super-resolution microwave imaging based on metasurface

PubMed Central

Wang, Libo; Li, Lianlin; Li, Yunbo; Zhang, Hao Chi; Cui, Tie Jun

2016-01-01

Real-time high-resolution (including super-resolution) imaging with low-cost hardware is a long sought-after goal in various imaging applications. Here, we propose broadband single-shot and single-sensor high-/super-resolution imaging by using a spatio-temporal dispersive metasurface and an imaging reconstruction algorithm. The metasurface with spatio-temporal dispersive property ensures the feasibility of the single-shot and single-sensor imager for super- and high-resolution imaging, since it can convert efficiently the detailed spatial information of the probed object into one-dimensional time- or frequency-dependent signal acquired by a single sensor fixed in the far-field region. The imaging quality can be improved by applying a feature-enhanced reconstruction algorithm in post-processing, and the desired imaging resolution is related to the distance between the object and metasurface. When the object is placed in the vicinity of the metasurface, the super-resolution imaging can be realized. The proposed imaging methodology provides a unique means to perform real-time data acquisition, high-/super-resolution images without employing expensive hardware (e.g. mechanical scanner, antenna array, etc.). We expect that this methodology could make potential breakthroughs in the areas of microwave, terahertz, optical, and even ultrasound imaging. PMID:27246668

High-Resolution Large Field-of-View FUV Compact Camera

NASA Technical Reports Server (NTRS)

Spann, James F.

2006-01-01

The need for a high resolution camera with a large field of view and capable to image dim emissions in the far-ultraviolet is driven by the widely varying intensities of FUV emissions and spatial/temporal scales of phenomena of interest in the Earth% ionosphere. In this paper, the concept of a camera is presented that is designed to achieve these goals in a lightweight package with sufficient visible light rejection to be useful for dayside and nightside emissions. The camera employs the concept of self-filtering to achieve good spectral resolution tuned to specific wavelengths. The large field of view is sufficient to image the Earth's disk at Geosynchronous altitudes and capable of a spatial resolution of >20 km. The optics and filters are emphasized.

High-resolution Bent-crystal Spectrometer for the Ultra-soft X-ray Region

DOE R&D Accomplishments Database

Beiersdorfer, P.; von Goeler, S.; Bitter, M.; Hill, K. W.; Hulse, R. A.; Walling, R. S.

1988-10-01

A multichannel vacuum Brag-crystal spectrometer has been developed for high-resolution measurements of the line emission from tokamak plasmas in the wavelength region between 4 and 25 angstrom. The spectrometer employs a bent crystal in Johann geometry and a microchannel-plate intensified photodiode array. The instrument is capable of measuring high-resolution spectra (lambda/..delta..lambda approx. 3000) with fast time resolution (4 msec per spectrum) and good spatial resolution (3 cm). The spectral bandwidth is ..delta..lambda/lambda{sub 0} = 8 angstrom. A simple tilt mechanism allows access to different wavelength intervals. In order to illustrate the utility of the new spectrometer, time- and space-resolved measurements of the n = 3 to n = 2 spectrum of selenium from the Princeton Large Torus tokamak plasmas are presented. The data are used to determine the plasma transport parameters and to infer the radial distribution of fluorinelike, neonlike, and sodiumlike ions of selenium in the plasma. The new ultra-soft x-ray spectrometer has thus enabled us to demonstrate the utility of high-resolution L-shell spectroscopy of neonlike ions as a fusion diagnostic.

Hyperlens-array-implemented optical microscopy

NASA Astrophysics Data System (ADS)

Iwanaga, Masanobu

2014-08-01

Limit of resolution of conventional optical microscopes has never reached below 100 nm under visible light illumination. We show that numerically designed high-transmittance hyperlens array (HLA) is implemented in an optical microscope and works in practice for achieving one-shot-recording optical images of in-situ placed objects with sub 50 nm resolution in lateral direction. Direct resolution test employing well-defined nanopatterns proves that the HLA-implemented imaging is super-resolution optical microscopy, which works even under nW/mm2 visible illumination for objects. The HLA implementation makes the resolution of conventional microscopes one-scale higher, leading to the 1/10 illumination wavelength range, that is, mesoscopic range.

Monitoring wound healing by multiphoton tomography/endoscopy

NASA Astrophysics Data System (ADS)

König, Karsten; Weinigel, Martin; Bückle, Rainer; Kaatz, Martin; Hipler, Christina; Zens, Katharina; Schneider, Stefan W.; Huck, Volker

2015-02-01

Certified clinical multiphoton tomographs are employed to perform rapid label-free high-resolution in vivo histology. Novel tomographs include a flexible 360° scan head attached to a mechano-optical arm for autofluorescence and SHG imaging as well as rigid two-photon GRIN microendoscope. Mitochondrial fluorescent NAD(P)H, fluorescent elastin, keratin, and melanin as well as SHG-active collagen can be imaged with submicron resolution in human skin. The system was employed to study the healing of chronic wounds (venous leg ulcer) and acute wounds (curettage of actinic or seborrheic keratosis) on a subcellular level. Furthermore, a flexible sterile foil as interface between wound and focusing optic was tested.

High resolution time-to-space conversion of sub-picosecond pulses at 1.55µm by non-degenerate SFG in PPLN crystal.

PubMed

Shayovitz, Dror; Herrmann, Harald; Sohler, Wolfgang; Ricken, Raimund; Silberhorn, Christine; Marom, Dan M

2012-11-19

We demonstrate high resolution and increased efficiency background-free time-to-space conversion using spectrally resolved non-degenerate and collinear SFG in a bulk PPLN crystal. A serial-to-parallel resolution factor of 95 and a time window of 42 ps were achieved. A 60-fold increase in conversion efficiency slope compared with our previous work using a BBO crystal [D. Shayovitz and D. M. Marom, Opt. Lett. 36, 1957 (2011)] was recorded. Finally the measured 40 GHz narrow linewidth of the output SFG signal implies the possibility to extract phase information by employing coherent detection techniques.

The STIS MAMA status: Current detector performance

NASA Technical Reports Server (NTRS)

Danks, A. C.; Joseph, C.; Bybee, R.; Argebright, V.; Abraham, J.; Kimble, R.; Woodgate, B.

1992-01-01

The STIS (Space Telescope Imaging Spectrograph) is a second generation Hubble instrument scheduled to fly in 1997. Through a variety of modes, the instrument will provide spectral resolutions from R approximately 50 in the objective spectroscopy mode to 100,000 in the high resolution echelle mode in the wavelength region from 115 to 1000 nm. In the UV the instrument employs two MAMA (Multimode Anode Microchannel plate Arrays) 1024 by 1024 pixel detectors, which provide high DQE (Detective Quantum Efficiency), and good dynamic range and resolution. The current progress and performance of these detectors are reported, illustrating that the technology is mature and that the performance is very close to flight requirements.

Interior tomography in microscopic CT with image reconstruction constrained by full field of view scan at low spatial resolution

NASA Astrophysics Data System (ADS)

Luo, Shouhua; Shen, Tao; Sun, Yi; Li, Jing; Li, Guang; Tang, Xiangyang

2018-04-01

In high resolution (microscopic) CT applications, the scan field of view should cover the entire specimen or sample to allow complete data acquisition and image reconstruction. However, truncation may occur in projection data and results in artifacts in reconstructed images. In this study, we propose a low resolution image constrained reconstruction algorithm (LRICR) for interior tomography in microscopic CT at high resolution. In general, the multi-resolution acquisition based methods can be employed to solve the data truncation problem if the project data acquired at low resolution are utilized to fill up the truncated projection data acquired at high resolution. However, most existing methods place quite strict restrictions on the data acquisition geometry, which greatly limits their utility in practice. In the proposed LRICR algorithm, full and partial data acquisition (scan) at low and high resolutions, respectively, are carried out. Using the image reconstructed from sparse projection data acquired at low resolution as the prior, a microscopic image at high resolution is reconstructed from the truncated projection data acquired at high resolution. Two synthesized digital phantoms, a raw bamboo culm and a specimen of mouse femur, were utilized to evaluate and verify performance of the proposed LRICR algorithm. Compared with the conventional TV minimization based algorithm and the multi-resolution scout-reconstruction algorithm, the proposed LRICR algorithm shows significant improvement in reduction of the artifacts caused by data truncation, providing a practical solution for high quality and reliable interior tomography in microscopic CT applications. The proposed LRICR algorithm outperforms the multi-resolution scout-reconstruction method and the TV minimization based reconstruction for interior tomography in microscopic CT.

Solid state high resolution multi-spectral imager CCD test phase

NASA Technical Reports Server (NTRS)

1973-01-01

The program consisted of measuring the performance characteristics of charge coupled linear imaging devices, and a study defining a multispectral imaging system employing advanced solid state photodetection techniques.

Solid immersion terahertz imaging with sub-wavelength resolution

NASA Astrophysics Data System (ADS)

Chernomyrdin, Nikita V.; Schadko, Aleksander O.; Lebedev, Sergey P.; Tolstoguzov, Viktor L.; Kurlov, Vladimir N.; Reshetov, Igor V.; Spektor, Igor E.; Skorobogatiy, Maksim; Yurchenko, Stanislav O.; Zaytsev, Kirill I.

2017-05-01

We have developed a method of solid immersion THz imaging—a non-contact technique employing the THz beam focused into evanescent-field volume and allowing strong reduction in the dimensions of THz caustic. We have combined numerical simulations and experimental studies to demonstrate a sub-wavelength 0.35λ0-resolution of the solid immersion THz imaging system compared to 0.85λ0-resolution of a standard imaging system, employing only an aspherical singlet. We have discussed the prospective of using the developed technique in various branches of THz science and technology, namely, for THz measurements of solid-state materials featuring sub-wavelength variations of physical properties, for highly accurate mapping of healthy and pathological tissues in THz medical diagnosis, for detection of sub-wavelength defects in THz non-destructive sensing, and for enhancement of THz nonlinear effects.

Report on the ESO Workshop ''Astronomy at High Angular Resolution''

NASA Astrophysics Data System (ADS)

Boffin, H.; Schmidtobreick, L.; Hussain, G.; Berger, J.-Ph.

2015-03-01

A workshop took place in Brussels in 2000 on astrotomography, a generic term for indirect mapping techniques that can be applied to a huge variety of astrophysical systems, ranging from planets, single stars and binaries to active galactic nuclei. It appeared to be timely to revisit the topic given the many past, recent and forthcoming improvements in telescopes and instrumentation. We therefore decided to repeat the astrotomography workshop, but to put it into the much broader context of high angular resolution astronomy. Many techniques, from lucky and speckle imaging, adaptive optics to interferometry, are now widely employed to achieve high angular resolution and they have led to an amazing number of new discoveries. A summary of the workshop themes is presented.

High Efficiency Multi-shot Interleaved Spiral-In/Out Acquisition for High Resolution BOLD fMRI

PubMed Central

Jung, Youngkyoo; Samsonov, Alexey A.; Liu, Thomas T.; Buracas, Giedrius T.

2012-01-01

Growing demand for high spatial resolution BOLD functional MRI faces a challenge of the spatial resolution vs. coverage or temporal resolution tradeoff, which can be addressed by methods that afford increased acquisition efficiency. Spiral acquisition trajectories have been shown to be superior to currently prevalent echo-planar imaging in terms of acquisition efficiency, and high spatial resolution can be achieved by employing multiple-shot spiral acquisition. The interleaved spiral in-out trajectory is preferred over spiral-in due to increased BOLD signal CNR and higher acquisition efficiency than that of spiral-out or non-interleaved spiral in/out trajectories (1), but to date applicability of the multi-shot interleaved spiral in-out for high spatial resolution imaging has not been studied. Herein we propose multi-shot interleaved spiral in-out acquisition and investigate its applicability for high spatial resolution BOLD fMRI. Images reconstructed from interleaved spiral-in and -out trajectories possess artifacts caused by differences in T2* decay, off-resonance and k-space errors associated with the two trajectories. We analyze the associated errors and demonstrate that application of conjugate phase reconstruction and spectral filtering can substantially mitigate these image artifacts. After applying these processing steps, the multishot interleaved spiral in-out pulse sequence yields high BOLD CNR images at in-plane resolution below 1x1 mm while preserving acceptable temporal resolution (4 s) and brain coverage (15 slices of 2 mm thickness). Moreover, this method yields sufficient BOLD CNR at 1.5 mm isotropic resolution for detection of activation in hippocampus associated with cognitive tasks (Stern memory task). The multi-shot interleaved spiral in-out acquisition is a promising technique for high spatial resolution BOLD fMRI applications. PMID:23023395

High density FTA plates serve as efficient long-term sample storage for HLA genotyping.

PubMed

Lange, V; Arndt, K; Schwarzelt, C; Boehme, I; Giani, A S; Schmidt, A H; Ehninger, G; Wassmuth, R

2014-02-01

Storage of dried blood spots (DBS) on high-density FTA(®) plates could constitute an appealing alternative to frozen storage. However, it remains controversial whether DBS are suitable for high-resolution sequencing of human leukocyte antigen (HLA) alleles. Therefore, we extracted DNA from DBS that had been stored for up to 4 years, using six different methods. We identified those extraction methods that recovered sufficient high-quality DNA for reliable high-resolution HLA sequencing. Further, we confirmed that frozen whole blood samples that had been stored for several years can be transferred to filter paper without compromising HLA genotyping upon extraction. Concluding, DNA derived from high-density FTA(®) plates is suitable for high-resolution HLA sequencing, provided that appropriate extraction protocols are employed. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Study on a High Compression Processing for Video-on-Demand e-learning System

NASA Astrophysics Data System (ADS)

Nomura, Yoshihiko; Matsuda, Ryutaro; Sakamoto, Ryota; Sugiura, Tokuhiro; Matsui, Hirokazu; Kato, Norihiko

The authors proposed a high-quality and small-capacity lecture-video-file creating system for distance e-learning system. Examining the feature of the lecturing scene, the authors ingeniously employ two kinds of image-capturing equipment having complementary characteristics : one is a digital video camera with a low resolution and a high frame rate, and the other is a digital still camera with a high resolution and a very low frame rate. By managing the two kinds of image-capturing equipment, and by integrating them with image processing, we can produce course materials with the greatly reduced file capacity : the course materials satisfy the requirements both for the temporal resolution to see the lecturer's point-indicating actions and for the high spatial resolution to read the small written letters. As a result of a comparative experiment, the e-lecture using the proposed system was confirmed to be more effective than an ordinary lecture from the viewpoint of educational effect.

Damage extraction of buildings in the 2015 Gorkha, Nepal earthquake from high-resolution SAR data

NASA Astrophysics Data System (ADS)

Yamazaki, Fumio; Bahri, Rendy; Liu, Wen; Sasagawa, Tadashi

2016-05-01

Satellite remote sensing is recognized as one of the effective tools for detecting and monitoring affected areas due to natural disasters. Since SAR sensors can capture images not only at daytime but also at nighttime and under cloud-cover conditions, they are especially useful at an emergency response period. In this study, multi-temporal high-resolution TerraSAR-X images were used for damage inspection of the Kathmandu area, which was severely affected by the April 25, 2015 Gorkha Earthquake. The SAR images obtained before and after the earthquake were utilized for calculating the difference and correlation coefficient of backscatter. The affected areas were identified by high values of the absolute difference and low values of the correlation coefficient. The post-event high-resolution optical satellite images were employed as ground truth data to verify our results. Although it was difficult to estimate the damage levels for individual buildings, the high resolution SAR images could illustrate their capability in detecting collapsed buildings at emergency response times.

Study of a high-resolution, 3D positioning cadmium zinc telluride detector for PET.

PubMed

Gu, Y; Matteson, J L; Skelton, R T; Deal, A C; Stephan, E A; Duttweiler, F; Gasaway, T M; Levin, C S

2011-03-21

This paper investigates the performance of 1 mm resolution cadmium zinc telluride (CZT) detectors for positron emission tomography (PET) capable of positioning the 3D coordinates of individual 511 keV photon interactions. The detectors comprise 40 mm × 40 mm × 5 mm monolithic CZT crystals that employ a novel cross-strip readout with interspersed steering electrodes to obtain high spatial and energy resolution. The study found a single anode FWHM energy resolution of 3.06 ± 0.39% at 511 keV throughout most of the detector volume. Improved resolution is expected with properly shielded front-end electronics. Measurements made using a collimated beam established the efficacy of the steering electrodes in facilitating enhanced charge collection across anodes, as well as a spatial resolution of 0.44 ± 0.07 mm in the direction orthogonal to the electrode planes. Finally, measurements based on coincidence electronic collimation yielded a point spread function with 0.78 ± 0.10 mm FWHM, demonstrating 1 mm spatial resolution capability transverse to the anodes-as expected from the 1 mm anode pitch. These findings indicate that the CZT-based detector concept has excellent performance and shows great promise for a high-resolution PET system.

Specialized ADR To Settle Faculty Employment Disputes.

ERIC Educational Resources Information Center

DiNardo, Lawrence C.; Sherrill, John A.; Palmer, Anna R.

2001-01-01

Presents an innovative approach to resolution of faculty employment disputes at institutions of higher education. Discusses the framework in which faculty employment issues arise, the current state of alternative dispute resolution (ADR) as it is relevant to employment disputes, and the substantial benefits which could be achieved by developing a…

Background contamination by coplanar polychlorinated biphenyls (PCBs) in trace level high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS) analytical procedures.

PubMed

Ferrario, J; Byrne, C; Dupuy, A E

1997-06-01

The addition of the "dioxin-like" polychlorinated biphenyl (PCB) congeners to the assessment of risk associated with the 2,3,7,8-chlorine substituted dioxins and furans has dramatically increased the number of laboratories worldwide that are developing analytical procedures for their detection and quantitation. Most of these procedures are based on established sample preparation and analytical techniques employing high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS), which are used for the analyses of dioxin/furans at low parts-per-trillion (ppt) levels. A significant and widespread problem that arises when using these sample preparation procedures for the analysis of coplanar PCBs is the presence of background levels of these congeners. Industrial processes, urban incineration, leaking electrical transformers, hazardous waste accidents, and improper waste disposal practices have released appreciable quantities of PCBs into the environment. This contamination has resulted in the global distribution of these compounds via the atmosphere and their ubiquitous presence in ambient air. The background presence of these compounds in method blanks must be addressed when determining the exact concentrations of these and other congeners in environmental samples. In this study reliable procedures were developed to accurately define these background levels and assess their variability over the course of the study. The background subtraction procedures developed and employed increase the probability that the values reported accurately represent the concentrations found in the samples and were not biased due to this background contamination.

Background contamination by coplanar polychlorinated biphenyls (PCBs) in trace level high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS) analytical procedures

NASA Technical Reports Server (NTRS)

Ferrario, J.; Byrne, C.; Dupuy, A. E. Jr

1997-01-01

The addition of the "dioxin-like" polychlorinated biphenyl (PCB) congeners to the assessment of risk associated with the 2,3,7,8-chlorine substituted dioxins and furans has dramatically increased the number of laboratories worldwide that are developing analytical procedures for their detection and quantitation. Most of these procedures are based on established sample preparation and analytical techniques employing high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS), which are used for the analyses of dioxin/furans at low parts-per-trillion (ppt) levels. A significant and widespread problem that arises when using these sample preparation procedures for the analysis of coplanar PCBs is the presence of background levels of these congeners. Industrial processes, urban incineration, leaking electrical transformers, hazardous waste accidents, and improper waste disposal practices have released appreciable quantities of PCBs into the environment. This contamination has resulted in the global distribution of these compounds via the atmosphere and their ubiquitous presence in ambient air. The background presence of these compounds in method blanks must be addressed when determining the exact concentrations of these and other congeners in environmental samples. In this study reliable procedures were developed to accurately define these background levels and assess their variability over the course of the study. The background subtraction procedures developed and employed increase the probability that the values reported accurately represent the concentrations found in the samples and were not biased due to this background contamination.

The coupling of high-speed high resolution experimental data and LES through data assimilation techniques

NASA Astrophysics Data System (ADS)

Harris, S.; Labahn, J. W.; Frank, J. H.; Ihme, M.

2017-11-01

Data assimilation techniques can be integrated with time-resolved numerical simulations to improve predictions of transient phenomena. In this study, optimal interpolation and nudging are employed for assimilating high-speed high-resolution measurements obtained for an inert jet into high-fidelity large-eddy simulations. This experimental data set was chosen as it provides both high spacial and temporal resolution for the three-component velocity field in the shear layer of the jet. Our first objective is to investigate the impact that data assimilation has on the resulting flow field for this inert jet. This is accomplished by determining the region influenced by the data assimilation and corresponding effect on the instantaneous flow structures. The second objective is to determine optimal weightings for two data assimilation techniques. The third objective is to investigate how the frequency at which the data is assimilated affects the overall predictions. Graduate Research Assistant, Department of Mechanical Engineering.

Cross-correlation photothermal optical coherence tomography with high effective resolution.

PubMed

Tang, Peijun; Liu, Shaojie; Chen, Junbo; Yuan, Zhiling; Xie, Bingkai; Zhou, Jianhua; Tang, Zhilie

2017-12-01

We developed a cross-correlation photothermal optical coherence tomography (CC-PTOCT) system for photothermal imaging with high lateral and axial resolution. The CC-PTOCT system consists of a phase-sensitive OCT system, a modulated pumping laser, and a digital cross-correlator. The pumping laser was used to induce the photothermal effect in the sample, causing a slight phase modulation of the OCT signals. A spatial phase differentiation method was employed to reduce phase accumulation. The noise brought by the phase differentiation method and the strong background noise were suppressed efficiently by the cross-correlator, which was utilized to extract the photothermal signals from the modulated signals. Combining the cross-correlation technique with spatial phase differentiation can improve both lateral and axial resolution of the PTOCT imaging system. Clear photothermal images of blood capillaries of a mouse ear in vivo were successfully obtained with high lateral and axial resolution. The experimental results demonstrated that this system can enhance the effective transverse resolution, effective depth resolution, and contrast of the PTOCT image effectively, aiding the ongoing development of the accurate 3D functional imaging.

Ab initio simulations of subatomic resolution images in noncontact atomic force microscopy

NASA Astrophysics Data System (ADS)

Kim, Minjung; Chelikowsky, James R.

2015-03-01

Direct imaging of polycyclic aromatic molecules with a subatomic resolution has recently been achieved with noncontact atomic force microscopy (nc-AFM). Specifically, nc-AFM employing a CO functionalized tip has provided details of the chemical bond in aromatic molecules, including the discrimination of bond order. However, the underlying physics of such high resolution imaging remains problematic. By employing new, efficient algorithms based on real space pseudopotentials, we calculate the forces between the nc-AFM tip and specimen. We simulate images of planar organic molecules with two different approaches: 1) with a chemically inert tip and 2) with a CO functionalized tip. We find dramatic differences in the resulting images, which are consistent with recent experimental work. Our work is supported by the DOE under DOE/DE-FG02-06ER46286 and by the Welch Foundation under Grant F-1837. Computational resources were provided by NERSC and XSEDE.

En-face Flying Spot OCT/Ophthalmoscope

NASA Astrophysics Data System (ADS)

Rosen, Richard B.; Garcia, Patricia; Podoleanu, Adrian Gh.; Cucu, Radu; Dobre, George; Trifanov, Irina; van Velthoven, Mirjam E. J.; de Smet, Marc D.; Rogers, John A.; Hathaway, Mark; Pedro, Justin; Weitz, Rishard

This is a review of a technique for high-resolution imaging of the eye that allows multiple sample sectioning perspectives with different axial resolutions. The technique involves the flying spot approach employed in confocal scanning laser ophthalmoscopy which is extended to OCT imaging via time domain en face fast lateral scanning. The ability of imaging with multiple axial resolutions stimulated the development of the dual en face OCT-confocal imaging technology. Dual imaging also allows various other imaging combinations, such as OCT with confocal microscopy for imaging the eye anterior segment and OCT with fluorescence angiography imaging.

A Virtual Study of Grid Resolution on Experiments of a Highly-Resolved Turbulent Plume

NASA Astrophysics Data System (ADS)

Maisto, Pietro M. F.; Marshall, Andre W.; Gollner, Michael J.; Fire Protection Engineering Department Collaboration

2017-11-01

An accurate representation of sub-grid scale turbulent mixing is critical for modeling fire plumes and smoke transport. In this study, PLIF and PIV diagnostics are used with the saltwater modeling technique to provide highly-resolved instantaneous field measurements in unconfined turbulent plumes useful for statistical analysis, physical insight, and model validation. The effect of resolution was investigated employing a virtual interrogation window (of varying size) applied to the high-resolution field measurements. Motivated by LES low-pass filtering concepts, the high-resolution experimental data in this study can be analyzed within the interrogation windows (i.e. statistics at the sub-grid scale) and on interrogation windows (i.e. statistics at the resolved scale). A dimensionless resolution threshold (L/D*) criterion was determined to achieve converged statistics on the filtered measurements. Such a criterion was then used to establish the relative importance between large and small-scale turbulence phenomena while investigating specific scales for the turbulent flow. First order data sets start to collapse at a resolution of 0.3D*, while for second and higher order statistical moments the interrogation window size drops down to 0.2D*.

Innovative Techniques for High-Resolution Imaging and Precision Tracking

DTIC Science & Technology

1990-04-20

field-of-view ladar. 6 𔄁 The bipath method employs two separate single-ended ladar systems to measure both the backscattering and extinction coefficients... Transmissometer measurements are very important not only for determining the overall accuracy of the proposed system but also for assessing its performance under...the maximum path length difference between the target elements. The necessary laser power can be deduced from the system resolution requirement. The

High-Resolution Light Transmission Spectroscopy of Nanoparticles in Real Time

NASA Astrophysics Data System (ADS)

Tanner, Carol; Sun, Nan; Deatsch, Alison; Li, Frank; Ruggiero, Steven

2017-04-01

As implemented here, Light Transmission Spectroscopy (LTS) is a high-resolution real-time technique for eliminating spectral noise and systematic effects in wide band spectroscopic measurements of nanoparticles. In this work, we combine LTS with spectral inversion for the purpose of characterizing the size, shape, and number of nanoparticles in solution. The apparatus employs a wide-band multi-wavelength light source and grating spectrometers coupled to CCD detectors. The light source ranges from 210 to 2000 nm, and the wavelength dependent light detection system ranges from 200 to 1100 nm with <=1 nm resolution. With this system, nanoparticles ranging from 1 to 3000 nm diameters can be studied. The nanoparticles are typically suspended in pure water or water-based buffer solutions. For testing and calibration purposes, results are presented for nanoparticles composed of polystyrene and gold. Mie theory is used to model the total extinction cross-section, and spectral inversion is employed to obtain quantitative particle size distributions. Discussed are the precision, accuracy, resolution, and sensitivity of our results. The technique is quite versatile and can be applied to spectroscopic investigations where wideband, accurate, low-noise, real-time spectra are desired. University of Notre Dame Office of Research, College of Science, Department of Physics, and USDA.

Absolute spectrophotometry of Titan, Uranus, and Neptune 3500-10,500 A

NASA Technical Reports Server (NTRS)

Neff, J. S.; Humm, D. C.; Bergstralh, J. T.; Cochran, A. L.; Cochran, W. D.; Barker, E. S.; Tull, R. G.

1984-01-01

The present absolute measurements of Titan, Uranus and Neptune geometric albedo spectra in the 3500-10,500 A range have a resolution of about 7 A, together with high SNR, in virtue of the exceptional effeciency of the spectrograph and Reticon detector employed. The high precision and spectral resolution of the data, which are in excellent agreement with the Uranus albedo measurements of Lockwood et al. (1983), make possible quantitative measurements of the effects of Raman scattering by H2 in the Uranus and Neptune atmospheres.

Large depth high-precision FMCW tomography using a distributed feedback laser array

NASA Astrophysics Data System (ADS)

DiLazaro, Thomas; Nehmetallah, George

2018-02-01

Swept-source optical coherence tomography (SS-OCT) has been widely employed in the medical industry for the high resolution imaging of subsurface biological structures. SS-OCT typically exhibits axial resolutions on the order of tens of microns at speeds of hundreds of kilohertz. Using the same coherent heterodyne detection technique, frequency modulated continuous wave (FMCW) ladar has been used for highly precise ranging for distances up to kilometers. Distributed feedback lasers (DFBs) have been used as a simple and inexpensive source for FMCW ranging. Here, we use a bandwidth-combined DFB array for sub-surface volume imaging at a 27 μm axial resolution over meters of distance. 2D and 3D tomographic images of several semi-transparent and diffuse objects at distances up to 10 m will be presented.

A method of extending the depth of focus of the high-resolution X-ray imaging system employing optical lens and scintillator: a phantom study.

PubMed

Li, Guang; Luo, Shouhua; Yan, Yuling; Gu, Ning

2015-01-01

The high-resolution X-ray imaging system employing synchrotron radiation source, thin scintillator, optical lens and advanced CCD camera can achieve a resolution in the range of tens of nanometers to sub-micrometer. Based on this advantage, it can effectively image tissues, cells and many other small samples, especially the calcification in the vascular or in the glomerulus. In general, the thickness of the scintillator should be several micrometers or even within nanometers because it has a big relationship with the resolution. However, it is difficult to make the scintillator so thin, and additionally thin scintillator may greatly reduce the efficiency of collecting photons. In this paper, we propose an approach to extend the depth of focus (DOF) to solve these problems. We develop equation sets by deducing the relationship between the high-resolution image generated by the scintillator and the degraded blur image due to defect of focus first, and then we adopt projection onto convex sets (POCS) and total variation algorithm to get the solution of the equation sets and to recover the blur image. By using a 20 μm thick unmatching scintillator to replace the 1 μm thick matching one, we simulated a high-resolution X-ray imaging system and got a degraded blur image. Based on the algorithm proposed, we recovered the blur image and the result in the experiment showed that the proposed algorithm has good performance on the recovery of image blur caused by unmatching thickness of scintillator. The method proposed is testified to be able to efficiently recover the degraded image due to defect of focus. But, the quality of the recovery image especially of the low contrast image depends on the noise level of the degraded blur image, so there is room for improving and the corresponding denoising algorithm is worthy for further study and discussion.

A method of extending the depth of focus of the high-resolution X-ray imaging system employing optical lens and scintillator: a phantom study

PubMed Central

2015-01-01

Background The high-resolution X-ray imaging system employing synchrotron radiation source, thin scintillator, optical lens and advanced CCD camera can achieve a resolution in the range of tens of nanometers to sub-micrometer. Based on this advantage, it can effectively image tissues, cells and many other small samples, especially the calcification in the vascular or in the glomerulus. In general, the thickness of the scintillator should be several micrometers or even within nanometers because it has a big relationship with the resolution. However, it is difficult to make the scintillator so thin, and additionally thin scintillator may greatly reduce the efficiency of collecting photons. Methods In this paper, we propose an approach to extend the depth of focus (DOF) to solve these problems. We develop equation sets by deducing the relationship between the high-resolution image generated by the scintillator and the degraded blur image due to defect of focus first, and then we adopt projection onto convex sets (POCS) and total variation algorithm to get the solution of the equation sets and to recover the blur image. Results By using a 20 μm thick unmatching scintillator to replace the 1 μm thick matching one, we simulated a high-resolution X-ray imaging system and got a degraded blur image. Based on the algorithm proposed, we recovered the blur image and the result in the experiment showed that the proposed algorithm has good performance on the recovery of image blur caused by unmatching thickness of scintillator. Conclusions The method proposed is testified to be able to efficiently recover the degraded image due to defect of focus. But, the quality of the recovery image especially of the low contrast image depends on the noise level of the degraded blur image, so there is room for improving and the corresponding denoising algorithm is worthy for further study and discussion. PMID:25602532

Median filters as a tool to determine dark noise thresholds in high resolution smartphone image sensors for scientific imaging

NASA Astrophysics Data System (ADS)

Igoe, Damien P.; Parisi, Alfio V.; Amar, Abdurazaq; Rummenie, Katherine J.

2018-01-01

An evaluation of the use of median filters in the reduction of dark noise in smartphone high resolution image sensors is presented. The Sony Xperia Z1 employed has a maximum image sensor resolution of 20.7 Mpixels, with each pixel having a side length of just over 1 μm. Due to the large number of photosites, this provides an image sensor with very high sensitivity but also makes them prone to noise effects such as hot-pixels. Similar to earlier research with older models of smartphone, no appreciable temperature effects were observed in the overall average pixel values for images taken in ambient temperatures between 5 °C and 25 °C. In this research, hot-pixels are defined as pixels with intensities above a specific threshold. The threshold is determined using the distribution of pixel values of a set of images with uniform statistical properties associated with the application of median-filters of increasing size. An image with uniform statistics was employed as a training set from 124 dark images, and the threshold was determined to be 9 digital numbers (DN). The threshold remained constant for multiple resolutions and did not appreciably change even after a year of extensive field use and exposure to solar ultraviolet radiation. Although the temperature effects' uniformity masked an increase in hot-pixel occurrences, the total number of occurrences represented less than 0.1% of the total image. Hot-pixels were removed by applying a median filter, with an optimum filter size of 7 × 7; similar trends were observed for four additional smartphone image sensors used for validation. Hot-pixels were also reduced by decreasing image resolution. The method outlined in this research provides a methodology to characterise the dark noise behavior of high resolution image sensors for use in scientific investigations, especially as pixel sizes decrease.

A 4.5 km resolution Arctic Ocean simulation with the global multi-resolution model FESOM 1.4

NASA Astrophysics Data System (ADS)

Wang, Qiang; Wekerle, Claudia; Danilov, Sergey; Wang, Xuezhu; Jung, Thomas

2018-04-01

In the framework of developing a global modeling system which can facilitate modeling studies on Arctic Ocean and high- to midlatitude linkage, we evaluate the Arctic Ocean simulated by the multi-resolution Finite Element Sea ice-Ocean Model (FESOM). To explore the value of using high horizontal resolution for Arctic Ocean modeling, we use two global meshes differing in the horizontal resolution only in the Arctic Ocean (24 km vs. 4.5 km). The high resolution significantly improves the model's representation of the Arctic Ocean. The most pronounced improvement is in the Arctic intermediate layer, in terms of both Atlantic Water (AW) mean state and variability. The deepening and thickening bias of the AW layer, a common issue found in coarse-resolution simulations, is significantly alleviated by using higher resolution. The topographic steering of the AW is stronger and the seasonal and interannual temperature variability along the ocean bottom topography is enhanced in the high-resolution simulation. The high resolution also improves the ocean surface circulation, mainly through a better representation of the narrow straits in the Canadian Arctic Archipelago (CAA). The representation of CAA throughflow not only influences the release of water masses through the other gateways but also the circulation pathways inside the Arctic Ocean. However, the mean state and variability of Arctic freshwater content and the variability of freshwater transport through the Arctic gateways appear not to be very sensitive to the increase in resolution employed here. By highlighting the issues that are independent of model resolution, we address that other efforts including the improvement of parameterizations are still required.

Bayesian Peptide Peak Detection for High Resolution TOF Mass Spectrometry.

PubMed

Zhang, Jianqiu; Zhou, Xiaobo; Wang, Honghui; Suffredini, Anthony; Zhang, Lin; Huang, Yufei; Wong, Stephen

2010-11-01

In this paper, we address the issue of peptide ion peak detection for high resolution time-of-flight (TOF) mass spectrometry (MS) data. A novel Bayesian peptide ion peak detection method is proposed for TOF data with resolution of 10 000-15 000 full width at half-maximum (FWHW). MS spectra exhibit distinct characteristics at this resolution, which are captured in a novel parametric model. Based on the proposed parametric model, a Bayesian peak detection algorithm based on Markov chain Monte Carlo (MCMC) sampling is developed. The proposed algorithm is tested on both simulated and real datasets. The results show a significant improvement in detection performance over a commonly employed method. The results also agree with expert's visual inspection. Moreover, better detection consistency is achieved across MS datasets from patients with identical pathological condition.

Bayesian Peptide Peak Detection for High Resolution TOF Mass Spectrometry

PubMed Central

Zhang, Jianqiu; Zhou, Xiaobo; Wang, Honghui; Suffredini, Anthony; Zhang, Lin; Huang, Yufei; Wong, Stephen

2011-01-01

In this paper, we address the issue of peptide ion peak detection for high resolution time-of-flight (TOF) mass spectrometry (MS) data. A novel Bayesian peptide ion peak detection method is proposed for TOF data with resolution of 10 000–15 000 full width at half-maximum (FWHW). MS spectra exhibit distinct characteristics at this resolution, which are captured in a novel parametric model. Based on the proposed parametric model, a Bayesian peak detection algorithm based on Markov chain Monte Carlo (MCMC) sampling is developed. The proposed algorithm is tested on both simulated and real datasets. The results show a significant improvement in detection performance over a commonly employed method. The results also agree with expert’s visual inspection. Moreover, better detection consistency is achieved across MS datasets from patients with identical pathological condition. PMID:21544266

Study of a high-resolution, 3-D positioning cadmium zinc telluride detector for PET

PubMed Central

Gu, Y; Matteson, J L; Skelton, R T; Deal, A C; Stephan, E A; Duttweiler, F; Gasaway, T M; Levin, C S

2011-01-01

This paper investigates the performance of 1 mm resolution Cadmium Zinc Telluride (CZT) detectors for positron emission tomography (PET) capable of positioning the 3-D coordinates of individual 511 keV photon interactions. The detectors comprise 40 mm × 40 mm × 5 mm monolithic CZT crystals that employ a novel cross-strip readout with interspersed steering electrodes to obtain high spatial and energy resolution. The study found a single anode FWHM energy resolution of 3.06±0.39% at 511 keV throughout most the detector volume. Improved resolution is expected with properly shielded front-end electronics. Measurements made using a collimated beam established the efficacy of the steering electrodes in facilitating enhanced charge collection across anodes, as well as a spatial resolution of 0.44±0.07 mm in the direction orthogonal to the electrode planes. Finally, measurements based on coincidence electronic collimation yielded a point spread function with 0.78±0.10 mm FWHM, demonstrating 1 mm spatial resolution capability transverse to the anodes – as expected from the 1 mm anode pitch. These findings indicate that the CZT-based detector concept has excellent performance and shows great promise for a high-resolution PET system. PMID:21335649

Obtaining high-resolution velocity spectra using weighted semblance

NASA Astrophysics Data System (ADS)

Ebrahimi, Saleh; Kahoo, Amin Roshandel; Porsani, Milton J.; Kalateh, Ali Nejati

2017-02-01

Velocity analysis employs coherency measurement along a hyperbolic or non-hyperbolic trajectory time window to build velocity spectra. Accuracy and resolution are strictly related to the method of coherency measurements. Semblance, the most common coherence measure, has poor resolution velocity which affects one's ability to distinguish and pick distinct peaks. Increase the resolution of the semblance velocity spectra causes the accuracy of estimated velocity for normal moveout correction and stacking is improved. The low resolution of semblance spectra depends on its low sensitivity to velocity changes. In this paper, we present a new weighted semblance method that ensures high-resolution velocity spectra. To increase the resolution of semblance spectra, we introduce two weighting functions based on the first to second singular values ratio of the time window and the position of the seismic wavelet in the time window to the semblance equation. We test the method on both synthetic and real field data to compare the resolution of weighted and conventional semblance methods. Numerical examples with synthetic and real seismic data indicate that the new proposed weighted semblance method provides higher resolution than conventional semblance and can separate the reflectors which are mixed in the semblance spectrum.

Fusion of shallow and deep features for classification of high-resolution remote sensing images

NASA Astrophysics Data System (ADS)

Gao, Lang; Tian, Tian; Sun, Xiao; Li, Hang

2018-02-01

Effective spectral and spatial pixel description plays a significant role for the classification of high resolution remote sensing images. Current approaches of pixel-based feature extraction are of two main kinds: one includes the widelyused principal component analysis (PCA) and gray level co-occurrence matrix (GLCM) as the representative of the shallow spectral and shape features, and the other refers to the deep learning-based methods which employ deep neural networks and have made great promotion on classification accuracy. However, the former traditional features are insufficient to depict complex distribution of high resolution images, while the deep features demand plenty of samples to train the network otherwise over fitting easily occurs if only limited samples are involved in the training. In view of the above, we propose a GLCM-based convolution neural network (CNN) approach to extract features and implement classification for high resolution remote sensing images. The employment of GLCM is able to represent the original images and eliminate redundant information and undesired noises. Meanwhile, taking shallow features as the input of deep network will contribute to a better guidance and interpretability. In consideration of the amount of samples, some strategies such as L2 regularization and dropout methods are used to prevent over-fitting. The fine-tuning strategy is also used in our study to reduce training time and further enhance the generalization performance of the network. Experiments with popular data sets such as PaviaU data validate that our proposed method leads to a performance improvement compared to individual involved approaches.

Correction of image drift and distortion in a scanning electron microscopy.

PubMed

Jin, P; Li, X

2015-12-01

Continuous research on small-scale mechanical structures and systems has attracted strong demand for ultrafine deformation and strain measurements. Conventional optical microscope cannot meet such requirements owing to its lower spatial resolution. Therefore, high-resolution scanning electron microscope has become the preferred system for high spatial resolution imaging and measurements. However, scanning electron microscope usually is contaminated by distortion and drift aberrations which cause serious errors to precise imaging and measurements of tiny structures. This paper develops a new method to correct drift and distortion aberrations of scanning electron microscope images, and evaluates the effect of correction by comparing corrected images with scanning electron microscope image of a standard sample. The drift correction is based on the interpolation scheme, where a series of images are captured at one location of the sample and perform image correlation between the first image and the consequent images to interpolate the drift-time relationship of scanning electron microscope images. The distortion correction employs the axial symmetry model of charged particle imaging theory to two images sharing with the same location of one object under different imaging fields of view. The difference apart from rigid displacement between the mentioned two images will give distortion parameters. Three-order precision is considered in the model and experiment shows that one pixel maximum correction is obtained for the employed high-resolution electron microscopic system. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

A Parallel Icosahedral, Higher Order Discontinuous Galerkin, Global Shallow Water Model: Global Ocean Tides and Aquaplanet Benchmarks

NASA Astrophysics Data System (ADS)

Salehipour, H.; Stuhne, G.; Peltier, W. R.

2012-12-01

The development of models of the ocean tides with higher resolution near the coastlines and courser mesh offshore, has been required due to the significant impacts of coastline configuration and bathymetry (associated with sea level rise) on the amplitude and phase of tidal constituents, not only under present conditions but also in the deep past [Griffiths and Peltier GRL 2008, Griffiths and Peltier AMS 2009, Hill et al. JGR 2011]. A global tidal model with enhanced resolution at the poles has been developed by Griffiths and Peltier [2008, 2009], which, although capable of highly resolving polar ocean tides , is based upon a standard structured Arakawa C grid and hence is not capable of resolving coastlines locally. Furthermore the use of a nested modelling approach, although it may enable local spatial refinement [Hill et al. 2011], nevertheless suffers from its inherent dependence on the availability of a global tidal model with necessarily low spatial resolution to provide the open boundary conditions required for the local high resolution model. On the other hand, an unstructured triangulation of the global domain provides a standalone framework that may be employed to study highly resolved regions without relying on secondary models. The first step in the development of the structure we are employing was described in Stuhne and Peltier [Ocean Modeling, 2009]. In further extending this modelling structure we are employing a new discontinuous Galerkin (DG) discretization of the governing equations in order to provide very high order of accuracy while also ensuring that momentum transport is locally conserved [Giraldo et al. JCP 2002]. After validating the 2D shallow water model with several test suites appropriate to aquaplanets [Williamson et al. JCP 1992, Galewsky et al. Tellus 2004, Nair and Lauritzen JCP 2010], the governing equations are extended to include the influence of internal tide drag in the deep ocean as well as the drag in shallow marginal seas together with the influence of gravitational self-attraction and loading. In this paper, we will explain the mathematical and numerical framework employed in the development of the DG global tidal model and present the validation results obtained using the present-day satellite altimetry data-constrained TPXO 6.2 global tidal solutions of Egbert et al. [JGR 1994].igure 1. Barotropic Instability Test of Galewsky et al. (Tellus 2004), with 2nd order DG

High-resolution angle-resolved photoemission study of electronic structure and charge-density wave formation in HoTe3

NASA Astrophysics Data System (ADS)

Liu, Guodong; Wang, Chenlu; Zhang, Yan; Hu, Bingfeng; Mou, Daixiang; Yu, Li; Zhao, Lin; Zhou, Xingjiang; Wang, Nanlin; Chen, Chuangtian; Xu, Zuyan

We performed high-resolution angle-resolved photoemission spectroscopy (ARPES) measurement on high quality crystal of HoTe3, an intriguing quasi-two-dimensional rare-earth-element tritelluride charge-density-wave (CDW) compound. The main features of the electronic structure in this compound are established by employing a quasi-CW laser (7eV) and a helium discharging lamp (21.22 eV) as excitation light sources. It reveals many bands back folded according to the CDW periodicity and two incommensurate CDW gaps created by perpendicular Fermi surface (FS) nesting vectors. A large gap is found to open in well nested regions of the Fermi surface sheets, whereas other Fermi surface sections with poor nesting remain ungapped. In particular, some peculiar features are identified by using our ultra-high resolution and bulk sensitive laser-ARPES.

Development of ultrahigh resolution alpha particle imaging detector using 1 mm channel size Si-PM array

NASA Astrophysics Data System (ADS)

Yamamoto, Seiichi; Kawaguchi, Wataru

2018-06-01

For precise distribution measurements of alpha particles, a high-resolution alpha particle imaging detector is required. Although combining a thin scintillator with a silicon photomultiplier (Si-PM) array is a promising method for achieving high resolution, the spatial resolution is limited. Reducing the size of the Si-PM array is a possible approach to improving the spatial resolution of the alpha particle imaging detector. Consequently, we employed a 1 mm channel size Si-PM array combined with a thin ZnS(Ag) sheet to form an alpha particle imaging detector and evaluated the performance. For the developed alpha particle imaging detector, an Si-PM array with 1 mm x 1 mm channel size arranged 8 x 8 was optically coupled to a ZnS(Ag) sheet with a 1-mm-thick light guide between them. The size of the alpha particle imaging detector was 9.5 mm x 9.5 mm. The spatial resolution of the developed alpha particle imaging detector was 0.14 mm FWHM, and the energy resolution was 74% FWHM for 5.5 MeV alpha particles. The uniformity of the imaging detector at the central part of the field of view (FOV) was ±4.7%. The background count rate was 0.06 counts/min. We obtained various high-resolution phantom images for alpha particles with the developed system. We conclude that the developed imaging detector is promising for high-resolution distribution measurements of alpha particles.

High efficiency multishot interleaved spiral-in/out: acquisition for high-resolution BOLD fMRI.

PubMed

Jung, Youngkyoo; Samsonov, Alexey A; Liu, Thomas T; Buracas, Giedrius T

2013-08-01

Growing demand for high spatial resolution blood oxygenation level dependent (BOLD) functional magnetic resonance imaging faces a challenge of the spatial resolution versus coverage or temporal resolution tradeoff, which can be addressed by methods that afford increased acquisition efficiency. Spiral acquisition trajectories have been shown to be superior to currently prevalent echo-planar imaging in terms of acquisition efficiency, and high spatial resolution can be achieved by employing multiple-shot spiral acquisition. The interleaved spiral in/out trajectory is preferred over spiral-in due to increased BOLD signal contrast-to-noise ratio (CNR) and higher acquisition efficiency than that of spiral-out or noninterleaved spiral in/out trajectories (Law & Glover. Magn Reson Med 2009; 62:829-834.), but to date applicability of the multishot interleaved spiral in/out for high spatial resolution imaging has not been studied. Herein we propose multishot interleaved spiral in/out acquisition and investigate its applicability for high spatial resolution BOLD functional magnetic resonance imaging. Images reconstructed from interleaved spiral-in and -out trajectories possess artifacts caused by differences in T2 decay, off-resonance, and k-space errors associated with the two trajectories. We analyze the associated errors and demonstrate that application of conjugate phase reconstruction and spectral filtering can substantially mitigate these image artifacts. After applying these processing steps, the multishot interleaved spiral in/out pulse sequence yields high BOLD CNR images at in-plane resolution below 1 × 1 mm while preserving acceptable temporal resolution (4 s) and brain coverage (15 slices of 2 mm thickness). Moreover, this method yields sufficient BOLD CNR at 1.5 mm isotropic resolution for detection of activation in hippocampus associated with cognitive tasks (Stern memory task). The multishot interleaved spiral in/out acquisition is a promising technique for high spatial resolution BOLD functional magnetic resonance imaging applications. © 2012 Wiley Periodicals, Inc.

Single-image super-resolution based on Markov random field and contourlet transform

NASA Astrophysics Data System (ADS)

Wu, Wei; Liu, Zheng; Gueaieb, Wail; He, Xiaohai

2011-04-01

Learning-based methods are well adopted in image super-resolution. In this paper, we propose a new learning-based approach using contourlet transform and Markov random field. The proposed algorithm employs contourlet transform rather than the conventional wavelet to represent image features and takes into account the correlation between adjacent pixels or image patches through the Markov random field (MRF) model. The input low-resolution (LR) image is decomposed with the contourlet transform and fed to the MRF model together with the contourlet transform coefficients from the low- and high-resolution image pairs in the training set. The unknown high-frequency components/coefficients for the input low-resolution image are inferred by a belief propagation algorithm. Finally, the inverse contourlet transform converts the LR input and the inferred high-frequency coefficients into the super-resolved image. The effectiveness of the proposed method is demonstrated with the experiments on facial, vehicle plate, and real scene images. A better visual quality is achieved in terms of peak signal to noise ratio and the image structural similarity measurement.

High resolution atomic force microscopy of double-stranded RNA.

PubMed

Ares, Pablo; Fuentes-Perez, Maria Eugenia; Herrero-Galán, Elías; Valpuesta, José M; Gil, Adriana; Gomez-Herrero, Julio; Moreno-Herrero, Fernando

2016-06-09

Double-stranded (ds) RNA mediates the suppression of specific gene expression, it is the genetic material of a number of viruses, and a key activator of the innate immune response against viral infections. The ever increasing list of roles played by dsRNA in the cell and its potential biotechnological applications over the last decade has raised an interest for the characterization of its mechanical properties and structure, and that includes approaches using Atomic Force Microscopy (AFM) and other single-molecule techniques. Recent reports have resolved the structure of dsDNA with AFM at unprecedented resolution. However, an equivalent study with dsRNA is still lacking. Here, we have visualized the double helix of dsRNA under near-physiological conditions and at sufficient resolution to resolve the A-form sub-helical pitch periodicity. We have employed different high-sensitive force-detection methods and obtained images with similar spatial resolution. Therefore, we show here that the limiting factors for high-resolution AFM imaging of soft materials in liquid medium are, rather than the imaging mode, the force between the tip and the sample and the sharpness of the tip apex.

High-resolution mass spectrometric analysis of biomass pyrolysis vapors

DOE PAGES

Christensen, Earl; Evans, Robert J.; Carpenter, Daniel

2017-01-19

Vapors generated from the pyrolysis of lignocellulosic biomass are made up of a complex mixture of oxygenated compounds. Direct analysis of these vapors provides insight into the mechanisms of depolymerization of cellulose, hemicellulose, and lignin as well as insight into reactions that may occur during condensation of pyrolysis vapors into bio-oil. Studies utilizing pyrolysis molecular beam mass spectrometry have provided valuable information regarding the chemical composition of pyrolysis vapors. Mass spectrometers generally employed with these instruments have low mass resolution of approximately a mass unit. The presence of chemical species with identical unit mass but differing elemental formulas cannot bemore » resolved with these instruments and are therefore detected as a single ion. In this study we analyzed the pyrolysis vapors of several biomass sources using a high-resolution double focusing mass spectrometer. High-resolution analysis of pyrolysis vapors allowed for speciation of several compounds that would be detected as a single ion with unit mass resolution. Lastly, these data not only provide greater detail into the composition of pyrolysis vapors but also highlight differences between vapors generated from multiple biomass feedstocks.« less

Rapid analysis of caffeine in various coffee samples employing direct analysis in real-time ionization-high-resolution mass spectrometry.

PubMed

Danhelova, Hana; Hradecky, Jaromir; Prinosilova, Sarka; Cajka, Tomas; Riddellova, Katerina; Vaclavik, Lukas; Hajslova, Jana

2012-07-01

The development and use of a fast method employing a direct analysis in real time (DART) ion source coupled to high-resolution time-of-flight mass spectrometry (TOFMS) for the quantitative analysis of caffeine in various coffee samples has been demonstrated in this study. A simple sample extraction procedure employing hot water was followed by direct, high-throughput (<1 min per run) examination of the extracts spread on a glass rod under optimized conditions of ambient mass spectrometry, without any prior chromatographic separation. For quantification of caffeine using DART-TOFMS, an external calibration was used. Isotopically labeled caffeine was used to compensate for the variations of the ion intensities of caffeine signal. Recoveries of the DART-TOFMS method were 97% for instant coffee at the spiking levels of 20 and 60 mg/g, respectively, while for roasted ground coffee, the obtained values were 106% and 107% at the spiking levels of 10 and 30 mg/g, respectively. The repeatability of the whole analytical procedure (expressed as relative standard deviation, RSD, %) was <5% for all tested spiking levels and matrices. Since the linearity range of the method was relatively narrow (two orders of magnitude), an optimization of sample dilution prior the DART-TOFMS measurement to avoid saturation of the detector was needed.

BLIPPED (BLIpped Pure Phase EncoDing) high resolution MRI with low amplitude gradients

NASA Astrophysics Data System (ADS)

Xiao, Dan; Balcom, Bruce J.

2017-12-01

MRI image resolution is proportional to the maximum k-space value, i.e. the temporal integral of the magnetic field gradient. High resolution imaging usually requires high gradient amplitudes and/or long spatial encoding times. Special gradient hardware is often required for high amplitudes and fast switching. We propose a high resolution imaging sequence that employs low amplitude gradients. This method was inspired by the previously proposed PEPI (π Echo Planar Imaging) sequence, which replaced EPI gradient reversals with multiple RF refocusing pulses. It has been shown that when the refocusing RF pulse is of high quality, i.e. sufficiently close to 180°, the magnetization phase introduced by the spatial encoding magnetic field gradient can be preserved and transferred to the following echo signal without phase rewinding. This phase encoding scheme requires blipped gradients that are identical for each echo, with low and constant amplitude, providing opportunities for high resolution imaging. We now extend the sequence to 3D pure phase encoding with low amplitude gradients. The method is compared with the Hybrid-SESPI (Spin Echo Single Point Imaging) technique to demonstrate the advantages in terms of low gradient duty cycle, compensation of concomitant magnetic field effects and minimal echo spacing, which lead to superior image quality and high resolution. The 3D imaging method was then applied with a parallel plate resonator RF probe, achieving a nominal spatial resolution of 17 μm in one dimension in the 3D image, requiring a maximum gradient amplitude of only 5.8 Gauss/cm.

High-magnification super-resolution FINCH microscopy using birefringent crystal lens interferometers

NASA Astrophysics Data System (ADS)

Siegel, Nisan; Lupashin, Vladimir; Storrie, Brian; Brooker, Gary

2016-12-01

Fresnel incoherent correlation holography (FINCH) microscopy is a promising approach for high-resolution biological imaging but has so far been limited to use with low-magnification, low-numerical-aperture configurations. We report the use of in-line incoherent interferometers made from uniaxial birefringent α-barium borate (α-BBO) or calcite crystals that overcome the aberrations and distortions present with previous implementations that employed spatial light modulators or gradient refractive index lenses. FINCH microscopy incorporating these birefringent elements and high-numerical-aperture oil immersion objectives could outperform standard wide-field fluorescence microscopy, with, for example, a 149 nm lateral point spread function at a wavelength of 590 nm. Enhanced resolution was confirmed with sub-resolution fluorescent beads. Taking the Golgi apparatus as a biological example, three different proteins labelled with GFP and two other fluorescent dyes in HeLa cells were resolved with an image quality that is comparable to similar samples captured by structured illumination microscopy.

Employing unmanned aerial vehicle to monitor the health condition of wind turbines

NASA Astrophysics Data System (ADS)

Huang, Yishuo; Chiang, Chih-Hung; Hsu, Keng-Tsang; Cheng, Chia-Chi

2018-04-01

Unmanned aerial vehicle (UAV) can gather the spatial information of huge structures, such as wind turbines, that can be difficult to obtain with traditional approaches. In this paper, the UAV used in the experiments is equipped with high resolution camera and thermal infrared camera. The high resolution camera can provide a series of images with resolution up to 10 Megapixels. Those images can be used to form the 3D model using the digital photogrammetry technique. By comparing the 3D scenes of the same wind turbine at different times, possible displacement of the supporting tower of the wind turbine, caused by ground movement or foundation deterioration may be determined. The recorded thermal images are analyzed by applying the image segmentation methods to the surface temperature distribution. A series of sub-regions are separated by the differences of the surface temperature. The high-resolution optical image and the segmented thermal image are fused such that the surface anomalies are more easily identified for wind turbines.

High-resolution multimodal clinical multiphoton tomography of skin

NASA Astrophysics Data System (ADS)

König, Karsten

2011-03-01

This review focuses on multimodal multiphoton tomography based on near infrared femtosecond lasers. Clinical multiphoton tomographs for 3D high-resolution in vivo imaging have been placed into the market several years ago. The second generation of this Prism-Award winning High-Tech skin imaging tool (MPTflex) was introduced in 2010. The same year, the world's first clinical CARS studies have been performed with a hybrid multimodal multiphoton tomograph. In particular, non-fluorescent lipids and water as well as mitochondrial fluorescent NAD(P)H, fluorescent elastin, keratin, and melanin as well as SHG-active collagen has been imaged with submicron resolution in patients suffering from psoriasis. Further multimodal approaches include the combination of multiphoton tomographs with low-resolution wide-field systems such as ultrasound, optoacoustical, OCT, and dermoscopy systems. Multiphoton tomographs are currently employed in Australia, Japan, the US, and in several European countries for early diagnosis of skin cancer, optimization of treatment strategies, and cosmetic research including long-term testing of sunscreen nanoparticles as well as anti-aging products.

A new synoptic scale resolving global climate simulation using the Community Earth System Model

NASA Astrophysics Data System (ADS)

Small, R. Justin; Bacmeister, Julio; Bailey, David; Baker, Allison; Bishop, Stuart; Bryan, Frank; Caron, Julie; Dennis, John; Gent, Peter; Hsu, Hsiao-ming; Jochum, Markus; Lawrence, David; Muñoz, Ernesto; diNezio, Pedro; Scheitlin, Tim; Tomas, Robert; Tribbia, Joseph; Tseng, Yu-heng; Vertenstein, Mariana

2014-12-01

High-resolution global climate modeling holds the promise of capturing planetary-scale climate modes and small-scale (regional and sometimes extreme) features simultaneously, including their mutual interaction. This paper discusses a new state-of-the-art high-resolution Community Earth System Model (CESM) simulation that was performed with these goals in mind. The atmospheric component was at 0.25° grid spacing, and ocean component at 0.1°. One hundred years of "present-day" simulation were completed. Major results were that annual mean sea surface temperature (SST) in the equatorial Pacific and El-Niño Southern Oscillation variability were well simulated compared to standard resolution models. Tropical and southern Atlantic SST also had much reduced bias compared to previous versions of the model. In addition, the high resolution of the model enabled small-scale features of the climate system to be represented, such as air-sea interaction over ocean frontal zones, mesoscale systems generated by the Rockies, and Tropical Cyclones. Associated single component runs and standard resolution coupled runs are used to help attribute the strengths and weaknesses of the fully coupled run. The high-resolution run employed 23,404 cores, costing 250 thousand processor-hours per simulated year and made about two simulated years per day on the NCAR-Wyoming supercomputer "Yellowstone."

High resolution T2(*)-weighted Magnetic Resonance Imaging at 3 Tesla using PROPELLER-EPI.

PubMed

Krämer, Martin; Reichenbach, Jürgen R

2014-05-01

We report the application of PROPELLER-EPI for high resolution T2(*)-weighted imaging with sub-millimeter in-plane resolution on a clinical 3 Tesla scanner. Periodically rotated blades of a long-axis PROPELLER-EPI sequence were acquired with fast gradient echo readout and acquisition matrix of 320 × 50 per blade. Images were reconstructed by using 2D-gridding, phase and geometric distortion correction and compensation of resonance frequency drifts that occurred during extended measurements. To characterize these resonance frequency offsets, short FID calibration measurements were added to the PROPELLER-EPI sequence. Functional PROPELLER-EPI was performed with volunteers using a simple block design of right handed finger tapping. Results indicate that PROPELLER-EPI can be employed for fast, high resolution T2(*)-weighted imaging provided geometric distortions and possible resonance frequency drifts are properly corrected. Even small resonance frequency drifts below 10 Hz as well as non-corrected geometric distortions degraded image quality substantially. In the initial fMRI experiment image quality and signal-to-noise ratio was sufficient for obtaining high resolution functional activation maps. Copyright © 2014. Published by Elsevier GmbH.

High-resolution surface analysis for extended-range downscaling with limited-area atmospheric models

NASA Astrophysics Data System (ADS)

Separovic, Leo; Husain, Syed Zahid; Yu, Wei; Fernig, David

2014-12-01

High-resolution limited-area model (LAM) simulations are frequently employed to downscale coarse-resolution objective analyses over a specified area of the globe using high-resolution computational grids. When LAMs are integrated over extended time frames, from months to years, they are prone to deviations in land surface variables that can be harmful to the quality of the simulated near-surface fields. Nudging of the prognostic surface fields toward a reference-gridded data set is therefore devised in order to prevent the atmospheric model from diverging from the expected values. This paper presents a method to generate high-resolution analyses of land-surface variables, such as surface canopy temperature, soil moisture, and snow conditions, to be used for the relaxation of lower boundary conditions in extended-range LAM simulations. The proposed method is based on performing offline simulations with an external surface model, forced with the near-surface meteorological fields derived from short-range forecast, operational analyses, and observed temperatures and humidity. Results show that the outputs of the surface model obtained in the present study have potential to improve the near-surface atmospheric fields in extended-range LAM integrations.

Robust mosiacs of close-range high-resolution images

NASA Astrophysics Data System (ADS)

Song, Ran; Szymanski, John E.

2008-03-01

This paper presents a robust algorithm which relies only on the information contained within the captured images for the construction of massive composite mosaic images from close-range and high-resolution originals, such as those obtained when imaging architectural and heritage structures. We first apply Harris algorithm to extract a selection of corners and, then, employ both the intensity correlation and the spatial correlation between the corresponding corners for matching them. Then we estimate the eight-parameter projective transformation matrix by the genetic algorithm. Lastly, image fusion using a weighted blending function together with intensity compensation produces an effective seamless mosaic image.

Achieving high spatial resolution using a microchannel plate detector with an economic and scalable approach

NASA Astrophysics Data System (ADS)

Wiggins, B. B.; deSouza, Z. O.; Vadas, J.; Alexander, A.; Hudan, S.; deSouza, R. T.

2017-11-01

A second generation position-sensitive microchannel plate detector using the induced signal approach has been realized. This detector is presently capable of measuring the incident position of electrons, photons, or ions. To assess the spatial resolution, the masked detector was illuminated by electrons. The initial, measured spatial resolution of 276 μm FWHM was improved by requiring a minimum signal amplitude on the anode and by employing digital signal processing techniques. The resulting measured spatial resolution of 119 μm FWHM corresponds to an intrinsic resolution of 98 μm FWHM when the effect of the finite slit width is de-convoluted. This measurement is a substantial improvement from the last reported spatial resolution of 466 μm FWHM using the induced signal approach. To understand the factors that limit the measured resolution, the performance of the detector is simulated.

High frequency, high time resolution time-to-digital converter employing passive resonating circuits.

PubMed

Ripamonti, Giancarlo; Abba, Andrea; Geraci, Angelo

2010-05-01

A method for measuring time intervals accurate to the picosecond range is based on phase measurements of oscillating waveforms synchronous with their beginning and/or end. The oscillation is generated by triggering an LC resonant circuit, whose capacitance is precharged. By using high Q resonators and a final active quenching of the oscillation, it is possible to conjugate high time resolution and a small measurement time, which allows a high measurement rate. Methods for fast analysis of the data are considered and discussed with reference to computing resource requirements, speed, and accuracy. Experimental tests show the feasibility of the method and a time accuracy better than 4 ps rms. Methods aimed at further reducing hardware resources are finally discussed.

Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles.

PubMed

Zhan, Qiuqiang; Liu, Haichun; Wang, Baoju; Wu, Qiusheng; Pu, Rui; Zhou, Chao; Huang, Bingru; Peng, Xingyun; Ågren, Hans; He, Sailing

2017-10-20

Stimulated emission depletion microscopy provides a powerful sub-diffraction imaging modality for life science studies. Conventionally, stimulated emission depletion requires a relatively high light intensity to obtain an adequate depletion efficiency through only light-matter interaction. Here we show efficient emission depletion for a class of lanthanide-doped upconversion nanoparticles with the assistance of interionic cross relaxation, which significantly lowers the laser intensity requirements of optical depletion. We demonstrate two-color super-resolution imaging using upconversion nanoparticles (resolution ~ 66 nm) with a single pair of excitation/depletion beams. In addition, we show super-resolution imaging of immunostained cytoskeleton structures of fixed cells (resolution ~ 82 nm) using upconversion nanoparticles. These achievements provide a new perspective for the development of photoswitchable luminescent probes and will broaden the applications of lanthanide-doped nanoparticles for sub-diffraction microscopic imaging.

Module for multiphoton high-resolution hyperspectral imaging and spectroscopy

NASA Astrophysics Data System (ADS)

Zeytunyan, Aram; Baldacchini, Tommaso; Zadoyan, Ruben

2018-02-01

We developed a module for dual-output, dual-wavelength lasers that facilitates multiphoton imaging and spectroscopy experiments and enables hyperspectral imaging with spectral resolution up to 5 cm-1. High spectral resolution is achieved by employing spectral focusing. Specifically, two sets of grating pairs are used to control the chirps in each laser beam. In contrast with the approach that uses fixed-length glass rods, grating pairs allow matching the spectral resolution and the linewidths of the Raman lines of interest. To demonstrate the performance of the module, we report the results of spectral focusing CARS and SRS microscopy experiments for various test samples and Raman shifts. The developed module can be used for a variety of multimodal imaging and spectroscopy applications, such as single- and multi-color two-photon fluorescence, second harmonic generation, third harmonic generation, pump-probe, transient absorption, and others.

Building Change Detection in Very High Resolution Satellite Stereo Image Time Series

NASA Astrophysics Data System (ADS)

Tian, J.; Qin, R.; Cerra, D.; Reinartz, P.

2016-06-01

There is an increasing demand for robust methods on urban sprawl monitoring. The steadily increasing number of high resolution and multi-view sensors allows producing datasets with high temporal and spatial resolution; however, less effort has been dedicated to employ very high resolution (VHR) satellite image time series (SITS) to monitor the changes in buildings with higher accuracy. In addition, these VHR data are often acquired from different sensors. The objective of this research is to propose a robust time-series data analysis method for VHR stereo imagery. Firstly, the spatial-temporal information of the stereo imagery and the Digital Surface Models (DSMs) generated from them are combined, and building probability maps (BPM) are calculated for all acquisition dates. In the second step, an object-based change analysis is performed based on the derivative features of the BPM sets. The change consistence between object-level and pixel-level are checked to remove any outlier pixels. Results are assessed on six pairs of VHR satellite images acquired within a time span of 7 years. The evaluation results have proved the efficiency of the proposed method.

Accelerated High-Resolution Differential Ion Mobility Separations Using Hydrogen

PubMed Central

Shvartsburg, Alexandre A.; Smith, Richard D.

2011-01-01

The resolving power of differential ion mobility spectrometry (FAIMS) was dramatically increased recently by carrier gases comprising up to 75% He or various vapors, enabling many new applications. However, the need for resolution of complex mixtures is virtually open-ended and many topical analyses demand yet finer separations. Also, the resolving power gains are often at the expense of speed, in particular making high-resolution FAIMS incompatible with online liquid-phase separations. Here, we report FAIMS employing hydrogen, specifically in mixtures with N2 containing up to 90% H2. Such compositions raise the mobilities of all ions and thus the resolving power beyond that previously feasible, while avoiding the electrical breakdown inevitable in He-rich mixtures. The increases in resolving power and ensuing peak resolution are especially significant at H2 fractions above ~50%. Higher resolution can be exchanged for acceleration of the analyses by up to ~4 times, at least. For more mobile species such as multiply-charged peptides, this exchange is presently forced by the constraints of existing FAIMS devices, but future designs optimized for H2 should consistently improve resolution for all analytes. PMID:22074292

First results of high-resolution modeling of Cenozoic subduction orogeny in Andes

NASA Astrophysics Data System (ADS)

Liu, S.; Sobolev, S. V.; Babeyko, A. Y.; Krueger, F.; Quinteros, J.; Popov, A.

2016-12-01

The Andean Orogeny is the result of the upper-plate crustal shortening during the Cenozoic Nazca plate subduction beneath South America plate. With up to 300 km shortening, the Earth's second highest Altiplano-Puna Plateau was formed with a pronounced N-S oriented deformation diversity. Furthermore, the tectonic shortening in the Southern Andes was much less intensive and started much later. The mechanism of the shortening and the nature of N-S variation of its magnitude remain controversial. The previous studies of the Central Andes suggested that they might be related to the N-S variation in the strength of the lithosphere, friction coupling at slab interface, and are probably influenced by the interaction of the climate and tectonic systems. However, the exact nature of the strength variation was not explored due to the lack of high numerical resolution and 3D numerical models at that time. Here we will employ large-scale subduction models with a high resolution to reveal and quantify the factors controlling the strength of lithospheric structures and their effect on the magnitude of tectonic shortening in the South America plate between 18°-35°S. These high-resolution models are performed by using the highly scalable parallel 3D code LaMEM (Lithosphere and Mantle Evolution Model). This code is based on finite difference staggered grid approach and employs massive linear and non-linear solvers within the PETSc library to complete high-performance MPI-based parallelization in geodynamic modeling. Currently, in addition to benchmark-models we are developing high-resolution (< 1km) 2D subduction models with application to Nazca-South America convergence. In particular, we will present the models focusing on the effect of friction reduction in the Paleozoic-Cenozoic sediments above the uppermost crust in the Subandean Ranges. Future work will be focused on the origin of different styles of deformation and topography evolution in Altiplano-Puna Plateau and Central-Southern Andes through 3D modeling of large-scale interaction of subducting and overriding plates.

Texture analysis of high-resolution FLAIR images for TLE

NASA Astrophysics Data System (ADS)

Jafari-Khouzani, Kourosh; Soltanian-Zadeh, Hamid; Elisevich, Kost

2005-04-01

This paper presents a study of the texture information of high-resolution FLAIR images of the brain with the aim of determining the abnormality and consequently the candidacy of the hippocampus for temporal lobe epilepsy (TLE) surgery. Intensity and volume features of the hippocampus from FLAIR images of the brain have been previously shown to be useful in detecting the abnormal hippocampus in TLE. However, the small size of the hippocampus may limit the texture information. High-resolution FLAIR images show more details of the abnormal intensity variations of the hippocampi and therefore are more suitable for texture analysis. We study and compare the low and high-resolution FLAIR images of six epileptic patients. The hippocampi are segmented manually by an expert from T1-weighted MR images. Then the segmented regions are mapped on the corresponding FLAIR images for texture analysis. The 2-D wavelet transforms of the hippocampi are employed for feature extraction. We compare the ability of the texture features from regular and high-resolution FLAIR images to distinguish normal and abnormal hippocampi. Intracranial EEG results as well as surgery outcome are used as gold standard. The results show that the intensity variations of the hippocampus are related to the abnormalities in the TLE.

The Advanced Telescope for High Energy Astrophysics

NASA Astrophysics Data System (ADS)

Guainazzi, Matteo

2017-08-01

Athena (the Advanced Telescope for High Energy Astrophysics) is a next generation X-ray observatory currently under study by ESA for launch in 2028. Athena is designed to address the Hot and Energetic Universe science theme, which addresses two key questions: 1) How did ordinary matter evolve into the large scale structures we see today? 2) How do black holes grow and shape the Universe. To address these topics Athena employs an innovative X-ray telescope based on Silicon Pore Optics technology to deliver extremely light weight and high throughput, while retaining excellent angular resolution. The mirror can be adjusted to focus onto one of two focal place instruments: the X-ray Integral Field Unit (X-IFU) which provides spatially-resolved, high resolution spectroscopy, and the Wide Field Imager (WFI) which provides spectral imaging over a large field of view, as well as high time resolution and count rate tolerance. Athena is currently in Phase A and the study status will be reviewed, along with the scientific motivations behind the mission.

A consensus embedding approach for segmentation of high resolution in vivo prostate magnetic resonance imagery

NASA Astrophysics Data System (ADS)

Viswanath, Satish; Rosen, Mark; Madabhushi, Anant

2008-03-01

Current techniques for localization of prostatic adenocarcinoma (CaP) via blinded trans-rectal ultrasound biopsy are associated with a high false negative detection rate. While high resolution endorectal in vivo Magnetic Resonance (MR) prostate imaging has been shown to have improved contrast and resolution for CaP detection over ultrasound, similarity in intensity characteristics between benign and cancerous regions on MR images contribute to a high false positive detection rate. In this paper, we present a novel unsupervised segmentation method that employs manifold learning via consensus schemes for detection of cancerous regions from high resolution 1.5 Tesla (T) endorectal in vivo prostate MRI. A significant contribution of this paper is a method to combine multiple weak, lower-dimensional representations of high dimensional feature data in a way analogous to classifier ensemble schemes, and hence create a stable and accurate reduced dimensional representation. After correcting for MR image intensity artifacts, such as bias field inhomogeneity and intensity non-standardness, our algorithm extracts over 350 3D texture features at every spatial location in the MR scene at multiple scales and orientations. Non-linear dimensionality reduction schemes such as Locally Linear Embedding (LLE) and Graph Embedding (GE) are employed to create multiple low dimensional data representations of this high dimensional texture feature space. Our novel consensus embedding method is used to average object adjacencies from within the multiple low dimensional projections so that class relationships are preserved. Unsupervised consensus clustering is then used to partition the objects in this consensus embedding space into distinct classes. Quantitative evaluation on 18 1.5 T prostate MR data against corresponding histology obtained from the multi-site ACRIN trials show a sensitivity of 92.65% and a specificity of 82.06%, which suggests that our method is successfully able to detect suspicious regions in the prostate.

In-line FINCH super resolution digital holographic fluorescence microscopy using a high efficiency transmission liquid crystal GRIN lens.

PubMed

Brooker, Gary; Siegel, Nisan; Rosen, Joseph; Hashimoto, Nobuyuki; Kurihara, Makoto; Tanabe, Ayano

2013-12-15

We report a new optical arrangement that creates high-efficiency, high-quality Fresnel incoherent correlation holography (FINCH) holograms using polarization sensitive transmission liquid crystal gradient index (TLCGRIN) diffractive lenses. In contrast, current universal practice in the field employs a reflective spatial light modulator (SLM) to separate sample and reference beams. Polarization sensitive TLCGRIN lenses enable a straight optical path, have >90% transmission efficiency, are not pixilated, and are free of many limitations of reflective SLM devices. For each sample point, two spherical beams created by a glass lens in combination with a polarization sensitive TLCGRIN lens interfere and create a hologram and resultant super resolution image.

Geographically explicit urban land use change scenarios for Mega cities: a case study in Tokyo

NASA Astrophysics Data System (ADS)

Yamagata, Y.; Bagan, H.; Seya, H.; Nakamichi, K.

2010-12-01

In preparation for the IPCC 5th assessment report, the international modeling community is developing four Representative Concentration Paths employing the scenarios developed by four different Integrated Assessment Models. These RCPs will be employed as an input to climate models, such as Earth System Models. In these days, the importance of assessment of not only global but also local (city/zone level) impacts of global change has gradually been recognized, thereby downscaling climate models are one of the urgent problems to be solved. Needless to say, reliable downscaling requires spatially high resolution land use change scenarios. So far, there has been proposed a lot of methods for constructing land use change scenarios with considering economic behavior of human, such as agent-based model (e.g., Parker et al., 2001), and land use transport (LUT) model (e.g., Anas and Liu, 2007). The latter approach in particular has widely been applied to actual urban/transport policy; hence modeling the interaction between them is very important for creating reliable land use change scenarios. However, the LUT models are usually built based on the zones of cities/municipalities whose spatial resolutions are too low to derive sensible parameters of the climate models. Moreover, almost all of the works which attempt to build spatially high resolution LUT model employs very small regions as the study area. The objective of this research is deriving various input parameters to climate models such as population density, fractional green vegetation cover, and anthropogenic heat emission with spatially high resolution land use change scenarios constructed with LUT model. The study area of this research is Tokyo metropolitan area, which is the largest urban area in the world (United Nations., 2010). Firstly, this study employs very high ground resolution zones composed of micro districts around 1km2. Secondly, the research attempt to combine remote sensing techniques and LUT models to derive future distribution of fractional green vegetation cover. The study has created two extreme land-use scenarios: urban concentration (compact city) and dispersion scenarios in order to show possible range of future land use change, and derives the input parameters for the climate models. The authors are planning to open the scenarios and derived parameters to relate researches. Anas, A. and Y. Liu. (2007). A Regional Economy, Land Use, and Transportation Model (REULU-TRAN): Formulation, Algorithm Design, and Testing. Journal of Regional Science, 47, 415-455. Parker, D.C., T. Berger, S.M. Manson, Editors (2001). Agent-Based Models of Land-Use and Land-Cover Change. LUCC Report Series No. 6, (Accessed: 27 AUG. 2009; http://www.globallandproject.org/Documents/LUCC_No_6.pdf) United Nations. (2010). World urbanization prospects: City population.

HPT: A High Spatial Resolution Multispectral Sensor for Microsatellite Remote Sensing

PubMed Central

Takahashi, Yukihiro; Sakamoto, Yuji; Kuwahara, Toshinori

2018-01-01

Although nano/microsatellites have great potential as remote sensing platforms, the spatial and spectral resolutions of an optical payload instrument are limited. In this study, a high spatial resolution multispectral sensor, the High-Precision Telescope (HPT), was developed for the RISING-2 microsatellite. The HPT has four image sensors: three in the visible region of the spectrum used for the composition of true color images, and a fourth in the near-infrared region, which employs liquid crystal tunable filter (LCTF) technology for wavelength scanning. Band-to-band image registration methods have also been developed for the HPT and implemented in the image processing procedure. The processed images were compared with other satellite images, and proven to be useful in various remote sensing applications. Thus, LCTF technology can be considered an innovative tool that is suitable for future multi/hyperspectral remote sensing by nano/microsatellites. PMID:29463022

Monolithic focused reference beam X-ray holography

PubMed Central

Geilhufe, J.; Pfau, B.; Schneider, M.; Büttner, F.; Günther, C. M.; Werner, S.; Schaffert, S.; Guehrs, E.; Frömmel, S.; Kläui, M.; Eisebitt, S.

2014-01-01

Fourier transform holography is a highly efficient and robust imaging method, suitable for single-shot imaging at coherent X-ray sources. In its common implementation, the image contrast is limited by the reference signal generated by a small pinhole aperture. Increased pinhole diameters improve the signal, whereas the resolution is diminished. Here we report a new concept to decouple the spatial resolution from the image contrast by employing a Fresnel zone plate to provide the reference beam. Superimposed on-axis images of distinct foci are separated with a novel algorithm. Our method is insensitive to mechanical drift or vibrations and allows for long integration times common at low-flux facilities like high harmonic generation sources. The application of monolithic focused reference beams improves the efficiency of high-resolution X-ray Fourier transform holography beyond all present approaches and paves the path towards sub-10 nm single-shot X-ray imaging. PMID:24394675

High Resolution Three-Dimensional MR Imaging of the Skull Base: Compartments, Boundaries, and Critical Structures.

PubMed

Blitz, Ari Meir; Aygun, Nafi; Herzka, Daniel A; Ishii, Masaru; Gallia, Gary L

2017-01-01

High-resolution 3D MRI of the skull base allows for a more detailed and accurate assessment of normal anatomic structures as well as the location and extent of skull base pathologies than has previously been possible. This article describes the techniques employed for high-resolution skull base MRI including pre- and post-contrast constructive interference in the steady state (CISS) imaging and their utility for evaluation of the many small structures of the skull base, focusing on those regions and concepts most pertinent to localization of cranial nerve palsies and in providing pre-operative guidance and post-operative assessment. The concept of skull base compartments as a means of conceptualizing the various layers of the skull base and their importance in assessment of masses of the skull base is discussed. Copyright © 2016 Elsevier Inc. All rights reserved.

Combining High Spatial Resolution Optical and LIDAR Data for Object-Based Image Classification

NASA Astrophysics Data System (ADS)

Li, R.; Zhang, T.; Geng, R.; Wang, L.

2018-04-01

In order to classify high spatial resolution images more accurately, in this research, a hierarchical rule-based object-based classification framework was developed based on a high-resolution image with airborne Light Detection and Ranging (LiDAR) data. The eCognition software is employed to conduct the whole process. In detail, firstly, the FBSP optimizer (Fuzzy-based Segmentation Parameter) is used to obtain the optimal scale parameters for different land cover types. Then, using the segmented regions as basic units, the classification rules for various land cover types are established according to the spectral, morphological and texture features extracted from the optical images, and the height feature from LiDAR respectively. Thirdly, the object classification results are evaluated by using the confusion matrix, overall accuracy and Kappa coefficients. As a result, a method using the combination of an aerial image and the airborne Lidar data shows higher accuracy.

Matrix Sublimation/Recrystallization for Imaging Proteins by Mass Spectrometry at High Spatial Resolution

PubMed Central

Yang, Junhai; Caprioli, Richard M.

2011-01-01

We have employed matrix deposition by sublimation for protein image analysis on tissue sections using a hydration/recrystallization process that produces high quality MALDI mass spectra and high spatial resolution ion images. We systematically investigated different washing protocols, the effect of tissue section thickness, the amount of sublimated matrix per unit area and different recrystallization conditions. The results show that an organic solvent rinse followed by ethanol/water rinses substantially increased sensitivity for the detection of proteins. Both the thickness of tissue section and amount of sinapinic acid sublimated per unit area have optimal ranges for maximal protein signal intensity. Ion images of mouse and rat brain sections at 50, 20 and 10 µm spatial resolution are presented and are correlated with H&E stained optical images. For targeted analysis, histology directed imaging can be performed using this protocol where MS analysis and H&E staining are performed on the same section. PMID:21639088

Analytical Applications Of High-Resolution Molecular Fluorescence Spectroscopy In Low Temperature Solid Matrices

NASA Astrophysics Data System (ADS)

Hofstraat, Johannes W.; van Zeijl, W. J.; Smedes, F.; Ariese, Freek; Gooijer, Cees; Velthorst, Nel H.; Locher, R.; Renn, Alois; Wild, Urs P.

1989-05-01

High-resolution fluorescence spectroscopy may be used to obtain highly specific, vibrationally resolved spectral signatures of molecules. Two techniques are presented that both make use of low temperature, solid matrices. In Shpol'skii spectroscopy highly resolved spectra are obtained by employing n-alkanes as solvents that form neat crystalline matrices at low temperatures in which the guest molecules occupy well defined substitutional sites. Fluorescence line-narrowing spectroscopy is based on the application of selective (mostly laser-) excitation of the guest molecules. Principles and analytical applications of both techniques will be discussed. Specific attention will be paid to the determination of pyrene in bird meat by means of Shpol'skii spectroscopy and to the possibilities of applying two-dimensional fluorescence line-narrowing spectroscopy.

Analysis and High-Resolution Modeling of Tropical Cyclogenesis During the TCS-08 and TPARC Field Campaign

DTIC Science & Technology

2014-10-13

synoptic and dynamic aspects of cyclogenesis, a multi-nested WRF model (with 2 km resolution in the innermost mesh) will be used to simulate both...intraseasonal and interannual variability of TC activity in the WNP. For the data assimilation task, WRF 3DVar assimilation system will be employed...simulated using WRF . This genesis is associated with Rossby wave energy dispersion of a pre- existing TC Bills (2000). Using the reanalysis data as an

A Terahertz VRT spectrometer employing quantum cascade lasers

NASA Astrophysics Data System (ADS)

Cole, William T. S.; Hlavacek, Nik C.; Lee, Alan W. M.; Kao, Tsung-Yu; Hu, Qing; Reno, John L.; Saykally, Richard J.

2015-10-01

The first application of a commercial Terahertz quantum cascade laser (QCL) system for high resolution spectroscopy of supersonic beams is presented. The QCLs exhibited continuous linear voltage tuning over a 2 GHz range about a center frequency of 3.762 THz with ∼1 ppm resolution. A sensitivity of ∼1 ppm fractional absorption was measured with a single pass optical system. Multipass operation at the quantum noise limit of the stressed photoconductor detector would produce a 100-fold improvement.

Improving depth resolutions in positron beam spectroscopy by concurrent ion-beam sputtering

NASA Astrophysics Data System (ADS)

John, Marco; Dalla, Ayham; Ibrahim, Alaa M.; Anwand, Wolfgang; Wagner, Andreas; Böttger, Roman; Krause-Rehberg, Reinhard

2018-05-01

The depth resolution of mono-energetic positron annihilation spectroscopy using a positron beam is shown to improve by concurrently removing the sample surface layer during positron beam spectroscopy. During ion-beam sputtering with argon ions, Doppler-broadening spectroscopy is performed with energies ranging from 3 keV to 5 keV allowing for high-resolution defect studies just below the sputtered surface. With this technique, significantly improved depth resolutions could be obtained even at larger depths when compared to standard positron beam experiments which suffer from extended positron implantation profiles at higher positron energies. Our results show that it is possible to investigate layered structures with a thickness of about 4 microns with significantly improved depth resolution. We demonstrated that a purposely generated ion-beam induced defect profile in a silicon sample could be resolved employing the new technique. A depth resolution of less than 100 nm could be reached.

Astronomy Simulation with Computer Graphics.

ERIC Educational Resources Information Center

Thomas, William E.

1982-01-01

"Planetary Motion Simulations" is a system of programs designed for students to observe motions of a superior planet (one whose orbit lies outside the orbit of the earth). Programs run on the Apple II microcomputer and employ high-resolution graphics to present the motions of Saturn. (Author/JN)

Simultaneous interrogation of interferometric and Bragg grating sensors

NASA Astrophysics Data System (ADS)

Brady, G.; Kalli, K.; Webb, D. J.; Jackson, D. A.; Reekie, L.; Archambault, J. L.

1995-06-01

We propose a new method for the simultaneous interrogation of conventional two-beam interferometers and Bragg grating sensors. The technique employs an unbalanced Mach-Zehnder interferometer illuminated by a single low-coherence source, which acts as a wavelength-tunable source for the grating and as a path-matched filter for the Fizeau interferometer, thus providing a high phase resolution output for each sensor. The grating sensor demonstrates a dynamic strain resolution of \\similar 0.05 mu 3 / \\radical Hz \\end-radical at 20 Hz, while the interferometric phase resolution is better than 1mrad/ \\radical Hz \\end-radical at 20 Hz, corresponding to an rms mirror displacement of 0.08 nm.

High-accuracy self-mixing interferometer based on multiple reflections using a simple external reflecting mirror

NASA Astrophysics Data System (ADS)

Wang, Xiu-lin; Wei, Zheng; Wang, Rui; Huang, Wen-cai

2018-05-01

A self-mixing interferometer (SMI) with resolution twenty times higher than that of a conventional interferometer is developed by multiple reflections. Only by employing a simple external reflecting mirror, the multiple-pass optical configuration can be constructed. The advantage of the configuration is simple and easy to make the light re-injected back into the laser cavity. Theoretical analysis shows that the resolution of measurement is scalable by adjusting the number of reflections. The experiment shows that the proposed method has the optical resolution of approximate λ/40. The influence of displacement sensitivity gain ( G) is further analyzed and discussed in practical experiments.

High quality high spatial resolution functional classification in low dose dynamic CT perfusion using singular value decomposition (SVD) and k-means clustering

NASA Astrophysics Data System (ADS)

Pisana, Francesco; Henzler, Thomas; Schönberg, Stefan; Klotz, Ernst; Schmidt, Bernhard; Kachelrieß, Marc

2017-03-01

Dynamic CT perfusion acquisitions are intrinsically high-dose examinations, due to repeated scanning. To keep radiation dose under control, relatively noisy images are acquired. Noise is then further enhanced during the extraction of functional parameters from the post-processing of the time attenuation curves of the voxels (TACs) and normally some smoothing filter needs to be employed to better visualize any perfusion abnormality, but sacrificing spatial resolution. In this study we propose a new method to detect perfusion abnormalities keeping both high spatial resolution and high CNR. To do this we first perform the singular value decomposition (SVD) of the original noisy spatial temporal data matrix to extract basis functions of the TACs. Then we iteratively cluster the voxels based on a smoothed version of the three most significant singular vectors. Finally, we create high spatial resolution 3D volumes where to each voxel is assigned a distance from the centroid of each cluster, showing how functionally similar each voxel is compared to the others. The method was tested on three noisy clinical datasets: one brain perfusion case with an occlusion in the left internal carotid, one healthy brain perfusion case, and one liver case with an enhancing lesion. Our method successfully detected all perfusion abnormalities with higher spatial precision when compared to the functional maps obtained with a commercially available software. We conclude this method might be employed to have a rapid qualitative indication of functional abnormalities in low dose dynamic CT perfusion datasets. The method seems to be very robust with respect to both spatial and temporal noise and does not require any special a priori assumption. While being more robust respect to noise and with higher spatial resolution and CNR when compared to the functional maps, our method is not quantitative and a potential usage in clinical routine could be as a second reader to assist in the maps evaluation, or to guide a dataset smoothing before the modeling part.

Simultaneous quantification of neuroactive dopamine serotonin and kynurenine pathway metabolites in gender-specific youth urine by ultra performance liquid chromatography tandem high resolution mass spectrometry.

PubMed

Lu, Haihua; Yu, Jing; Wang, Jun; Wu, Linlin; Xiao, Hang; Gao, Rong

2016-04-15

Neuroactive metabolites in dopamine, serotonin and kynurenine metabolic pathways play key roles in several physiological processes and their imbalances have been implicated in the pathophysiology of a wide range of disorders. The association of these metabolites' alterations with various pathologies has raised interest in analytical methods for accurate quantification in biological fluids. However, simultaneous measurement of various neuroactive metabolites represents great challenges due to their trace level, high polarity and instability. In this study, an analytical method was developed and validated for accurately quantifying 12 neuroactive metabolites covering three metabolic pathways in youth urine by ultra performance liquid chromatography coupled to electrospray tandem high resolution mass spectrometry (UPLC-ESI-HRMS/MS). The strategy of dansyl chloride derivatization followed by solid phase extraction on C18 cartridges were employed to reduce matrix interference and improve the extraction efficiency. The reverse phase chromatographic separation was achieved with a gradient elution program in 20 min. The high resolution mass spectrometer (Q Exactive) was employed, with confirmation and quantification by Target-MS/MS scan mode. Youth urine samples collected from 100 healthy volunteers (Female:Male=1:1) were analyzed to explore the differences in metabolite profile and their turnover between genders. The results demonstrated that the UPLC-ESI-HRMS/MS method is sensitive and robust, suitable for monitoring a large panel of metabolites and for discovering new biomarkers in the medical fields. Copyright © 2016 Elsevier B.V. All rights reserved.

High-Resolution Surface Reconstruction from Imagery for Close Range Cultural Heritage Applications

NASA Astrophysics Data System (ADS)

Wenzel, K.; Abdel-Wahab, M.; Cefalu, A.; Fritsch, D.

2012-07-01

The recording of high resolution point clouds with sub-mm resolution is a demanding and cost intensive task, especially with current equipment like handheld laser scanners. We present an image based approached, where techniques of image matching and dense surface reconstruction are combined with a compact and affordable rig of off-the-shelf industry cameras. Such cameras provide high spatial resolution with low radiometric noise, which enables a one-shot solution and thus an efficient data acquisition while satisfying high accuracy requirements. However, the largest drawback of image based solutions is often the acquisition of surfaces with low texture where the image matching process might fail. Thus, an additional structured light projector is employed, represented here by the pseudo-random pattern projector of the Microsoft Kinect. Its strong infrared-laser projects speckles of different sizes. By using dense image matching techniques on the acquired images, a 3D point can be derived for almost each pixel. The use of multiple cameras enables the acquisition of a high resolution point cloud with high accuracy for each shot. For the proposed system up to 3.5 Mio. 3D points with sub-mm accuracy can be derived per shot. The registration of multiple shots is performed by Structure and Motion reconstruction techniques, where feature points are used to derive the camera positions and rotations automatically without initial information.

SiC detectors to monitor ionizing radiations emitted from nuclear events and plasmas

NASA Astrophysics Data System (ADS)

Torrisi, L.; Cannavò, A.

2016-09-01

Silicon Carbide (SiC) semiconductor detectors are increasingly employed in Nuclear Physics for their advantages with respect to traditional silicon (Si). Such detectors show an energy resolution, charge mobility, response velocity and detection efficiency similar to Si detectors. However, the higher band gap (3.26 eV), the lower leakage current (∼10 pA) maintained also at room temperature, the higher radiation hardness and the higher density with respect to Si represent some indisputable advantages characterizing such detectors. The devices can be employed at high temperatures, at high absorbed doses and in the case of high visible light intensities, for example, in plasma, for limited exposition times without damage. Generally SiC Schottky diodes are employed in reverse polarization with an active region depth of the order of 100 µm, purity below 1014 cm-3 and an active area lower than 1 cm2. Measurements in the regime of proportionality with the radiation energy released in the active region and measurements in time-of-flight configuration are employed for nuclear emission events produced at both low and high fluences. Alpha spectra demonstrated an energy resolution of about 1.3% at 5.8 MeV. Radiation emission from laser-generated plasma can be monitored in terms of detected photons, electrons and ions, using the laser pulse as a start signal and the radiation detection as a stop signal, enabling to measure the ion velocity by knowing the target-detector flight distance. SiC spectra acquired in the Messina University laboratories using radioactive ion sources and at the PALS laboratory facility in Prague (Czech Republic) are presented. A preliminary study of the use of SiC detectors, embedded in a water equivalent polymer, as a dosimeter is presented and discussed.

KMC-1: a high resolution and high flux soft x-ray beamline at BESSY.

PubMed

Schaefers, F; Mertin, M; Gorgoi, M

2007-12-01

The crystal monochromator beamline KMC-1 at a BESSY II bending magnet covers the energy range from soft (1.7 keV) to hard x-rays (12 keV) employing the (n,-n) double crystal arrangement with constant beam offset. The monochromator is equipped with three sets of crystals, InSb, Si (111), and Si (422) which are exchangeable in situ within a few minutes. Beamline and monochromator have been optimized for high flux and high resolution. This could be achieved by (1) a windowless setup under ultrahigh-vacuum conditions up to the experiment, (2) by the use of only three optical elements to minimize reflection losses, (3) by collecting an unusually large horizontal radiation fan (6 mrad) with the toroidal premirror, and (4) the optimization of the crystal optics to the soft x-ray range necessitating quasibackscattering crystal geometry (theta(Bragg,max)=82 degrees) delivering crystal limited resolution. The multipurpose beamline is in use for a variety of user facilities such as extended x-ray absorption fine structure, ((Bio-)EXAFS) near-edge x-ray absorption fine structure (NEXAFS), absorption and fluorescence spectroscopy. Due to the windowless UHV setup the k edges of the technologically and biologically important elements such as Si, P, and S are accessible. In addition to these experiments this beamline is now extensively used for photoelectron spectroscopy at high kinetic energies. Photon flux in the 10(11)-10(12) photons/s range and beamline resolving powers of more than E/DeltaE approximately 100.000 have been measured at selected energies employing Si (nnn) high order radiation in quasibackscattering geometry, thus photoelectron spectroscopy with a total instrumental resolution of about 150 meV is possible. This article describes the design features of the beamline and reports some experimental results in the above mentioned fields.

KMC-1: A high resolution and high flux soft x-ray beamline at BESSY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schaefers, F.; Mertin, M.; Gorgoi, M.

2007-12-15

The crystal monochromator beamline KMC-1 at a BESSY II bending magnet covers the energy range from soft (1.7 keV) to hard x-rays (12 keV) employing the (n,-n) double crystal arrangement with constant beam offset. The monochromator is equipped with three sets of crystals, InSb, Si (111), and Si (422) which are exchangeable in situ within a few minutes. Beamline and monochromator have been optimized for high flux and high resolution. This could be achieved by (1) a windowless setup under ultrahigh-vacuum conditions up to the experiment, (2) by the use of only three optical elements to minimize reflection losses, (3)more » by collecting an unusually large horizontal radiation fan (6 mrad) with the toroidal premirror, and (4) the optimization of the crystal optics to the soft x-ray range necessitating quasibackscattering crystal geometry ({theta}{sub Bragg,max}=82 deg.) delivering crystal limited resolution. The multipurpose beamline is in use for a variety of user facilities such as extended x-ray absorption fine structure, ((Bio-)EXAFS) near-edge x-ray absorption fine structure (NEXAFS), absorption and fluorescence spectroscopy. Due to the windowless UHV setup the k edges of the technologically and biologically important elements such as Si, P, and S are accessible. In addition to these experiments this beamline is now extensively used for photoelectron spectroscopy at high kinetic energies. Photon flux in the 10{sup 11}-10{sup 12} photons/s range and beamline resolving powers of more than E/{delta}E{approx_equal}100.000 have been measured at selected energies employing Si (nnn) high order radiation in quasibackscattering geometry, thus photoelectron spectroscopy with a total instrumental resolution of about 150 meV is possible. This article describes the design features of the beamline and reports some experimental results in the above mentioned fields.« less

Occurrence of weak, sub-micron, tropospheric aerosol events at high Arctic latitudes

NASA Astrophysics Data System (ADS)

O'Neill, N. T.; Pancrati, O.; Baibakov, K.; Eloranta, E.; Batchelor, R. L.; Freemantle, J.; McArthur, L. J. B.; Strong, K.; Lindenmaier, R.

2008-07-01

Numerous fine mode (sub-micron) aerosol optical events were observed during the summer of 2007 at the High Arctic atmospheric observatory (PEARL) located at Eureka, Nunavut, Canada. Half of these events could be traced to forest fires in southern and eastern Russia and the Northwest Territories of Canada. The most notable findings were that (a) a combination of ground-based measurements (passive sunphotometry, high spectral resolution lidar) could be employed to determine that weak (near sub-visual) fine mode events had occurred, and (b) this data combined with remote sensing imagery products (MODIS, OMI-AI, FLAMBE fire sources), Fourier transform spectroscopy and back trajectories could be employed to identify the smoke events.

Spatially detailed retrievals of spring phenology from single-season high-resolution image time series

NASA Astrophysics Data System (ADS)

Vrieling, Anton; Skidmore, Andrew K.; Wang, Tiejun; Meroni, Michele; Ens, Bruno J.; Oosterbeek, Kees; O'Connor, Brian; Darvishzadeh, Roshanak; Heurich, Marco; Shepherd, Anita; Paganini, Marc

2017-07-01

Vegetation indices derived from satellite image time series have been extensively used to estimate the timing of phenological events like season onset. Medium spatial resolution (≥250 m) satellite sensors with daily revisit capability are typically employed for this purpose. In recent years, phenology is being retrieved at higher resolution (≤30 m) in response to increasing availability of high-resolution satellite data. To overcome the reduced acquisition frequency of such data, previous attempts involved fusion between high- and medium-resolution data, or combinations of multi-year acquisitions in a single phenological reconstruction. The objectives of this study are to demonstrate that phenological parameters can now be retrieved from single-season high-resolution time series, and to compare these retrievals against those derived from multi-year high-resolution and single-season medium-resolution satellite data. The study focuses on the island of Schiermonnikoog, the Netherlands, which comprises a highly-dynamic saltmarsh, dune vegetation, and agricultural land. Combining NDVI series derived from atmospherically-corrected images from RapidEye (5 m-resolution) and the SPOT5 Take5 experiment (10m-resolution) acquired between March and August 2015, phenological parameters were estimated using a function fitting approach. We then compared results with phenology retrieved from four years of 30 m Landsat 8 OLI data, and single-year 100 m Proba-V and 250 m MODIS temporal composites of the same period. Retrieved phenological parameters from combined RapidEye/SPOT5 displayed spatially consistent results and a large spatial variability, providing complementary information to existing vegetation community maps. Retrievals that combined four years of Landsat observations into a single synthetic year were affected by the inclusion of years with warmer spring temperatures, whereas adjustment of the average phenology to 2015 observations was only feasible for a few pixels due to cloud cover around phenological transition dates. The Proba-V and MODIS phenology retrievals scaled poorly relative to their high-resolution equivalents, indicating that medium-resolution phenology retrievals need to be interpreted with care, particularly in landscapes with fine-scale land cover variability.

Impact of patient navigation in eliminating economic disparities in cancer care.

PubMed

Rodday, Angie Mae; Parsons, Susan K; Snyder, Frederick; Simon, Melissa A; Llanos, Adana A M; Warren-Mears, Victoria; Dudley, Donald; Lee, Ji-Hyun; Patierno, Steven R; Markossian, Talar W; Sanders, Mechelle; Whitley, Elizabeth M; Freund, Karen M

2015-11-15

Patient navigation may reduce cancer disparities associated with socioeconomic status (SES) and household factors. This study examined whether these factors were associated with delays in diagnostic resolution among patients with cancer screening abnormalities and whether patient navigation ameliorated these delays. This study analyzed data from 5 of 10 centers of the National Cancer Institute's Patient Navigation Research Program, which collected SES and household data on employment, income, education, housing, marital status, and household composition. The primary outcome was the time to diagnostic resolution after a cancer screening abnormality. Separate adjusted Cox proportional hazard models were fit for each SES and household factor, and an interaction between that factor and the intervention status was included. Among the 3777 participants (1968 in the control arm and 1809 in the navigation intervention arm), 91% were women, and the mean age was 44 years; 43% were Hispanic, 28% were white, and 27% were African American. Within the control arm, the unemployed experienced a longer time to resolution than those employed full-time (hazard ratio [HR], 0.85; P = .02). Renters (HR, 0.81; P = .02) and those with other (ie, unstable) housing (HR, 0.60; P < .001) had delays in comparison with homeowners. Never married (HR, 0.70; P < .001) and previously married participants (HR, 0.85; P = .03) had a longer time to care than married participants. There were no differences in the time to diagnostic resolution with any of these variables within the navigation intervention arm. Delays in diagnostic resolution exist by employment, housing type, and marital status. Patient navigation eliminated these disparities in the study sample. These findings demonstrate the value of providing patient navigation to patients at high risk for delays in cancer care. © 2015 American Cancer Society.

The impact of patient navigation in eliminating economic disparities in cancer care

PubMed Central

Rodday, Angie Mae; Parsons, Susan K.; Snyder, Frederick; Simon, Melissa A.; Llanos, Adana A.M.; Warren-Mears, Victoria; Dudley, Donald; Lee, Ji-Hyun; Patierno, Steve R.; Markossian, Talar W.; Sanders, Mechelle; Whitley, Elizabeth; Freund, Karen M.

2015-01-01

Background Patient navigation may reduce cancer disparities associated with socioeconomic status (SES) and household factors. We examined whether these factors were associated with delays in diagnostic resolution among patients with cancer screening abnormalities and whether patient navigation ameliorated these delays. Methods We analyzed data from five of ten centers from the NCI Patient Navigation Research Program that collected SES and household data on employment, income, education, housing, marital status, and household composition. The primary outcome was time to diagnostic resolution following a cancer screening abnormality. We fit separate adjusted Cox proportional hazard models for each SES and household factor and included an interaction between that factor and intervention status. Results Among 3777 participants (n=1968 control, n=1809 navigation intervention), 91% were women, with a mean age of 44 years, and 43% were Hispanic, 28% White, and 27% African American. Within the control arm, the unemployed experienced longer time to resolution than the full-time employed (HR=0.85, p=0.02). Renters (HR=0.81, p=0.02) and those with other (i.e., unstable) housing (HR=0.60, p<0.001) had delays compared to homeowners. Never married (HR=0.70, p<0.001) and previously married participants (HR=0.85, p=0.03) had longer time to care than married participants. There were no differences in time to diagnostic resolution by any of these variables within the navigation intervention arm. Conclusions Delays in diagnostic resolution exist by employment, housing type, and marital status. Patient navigation eliminated these disparities in our study sample. Our findings demonstrate the value of providing patient navigation to patients at high risk for delays in cancer care. PMID:26348120

Single image super-resolution using self-optimizing mask via fractional-order gradient interpolation and reconstruction.

PubMed

Yang, Qi; Zhang, Yanzhu; Zhao, Tiebiao; Chen, YangQuan

2017-04-04

Image super-resolution using self-optimizing mask via fractional-order gradient interpolation and reconstruction aims to recover detailed information from low-resolution images and reconstruct them into high-resolution images. Due to the limited amount of data and information retrieved from low-resolution images, it is difficult to restore clear, artifact-free images, while still preserving enough structure of the image such as the texture. This paper presents a new single image super-resolution method which is based on adaptive fractional-order gradient interpolation and reconstruction. The interpolated image gradient via optimal fractional-order gradient is first constructed according to the image similarity and afterwards the minimum energy function is employed to reconstruct the final high-resolution image. Fractional-order gradient based interpolation methods provide an additional degree of freedom which helps optimize the implementation quality due to the fact that an extra free parameter α-order is being used. The proposed method is able to produce a rich texture detail while still being able to maintain structural similarity even under large zoom conditions. Experimental results show that the proposed method performs better than current single image super-resolution techniques. Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.

Application of CARS to scramjet combustion

NASA Technical Reports Server (NTRS)

Antcliff, R. R.

1987-01-01

A coherent anti-Stokes Raman spectroscopic (CARS) instrument has been developed for measuring simultaneously temperature and N2 - O2 species concentration in hostile flame environments. A folded BOXCARS arrangement was employed to obtain high spatial resolution. Polarization discrimination against the nonresonant background decreased the lower limits of O2 detectivity. The instrument has been primarily employed for validation of computational fluid-dynamics computer-model codes. Comparisons have been made to both the CHARNAL and TEACH codes on a hydrogen diffusion flame with good results.

Structural studies of RNA-protein complexes: A hybrid approach involving hydrodynamics, scattering, and computational methods.

PubMed

Patel, Trushar R; Chojnowski, Grzegorz; Astha; Koul, Amit; McKenna, Sean A; Bujnicki, Janusz M

2017-04-15

The diverse functional cellular roles played by ribonucleic acids (RNA) have emphasized the need to develop rapid and accurate methodologies to elucidate the relationship between the structure and function of RNA. Structural biology tools such as X-ray crystallography and Nuclear Magnetic Resonance are highly useful methods to obtain atomic-level resolution models of macromolecules. However, both methods have sample, time, and technical limitations that prevent their application to a number of macromolecules of interest. An emerging alternative to high-resolution structural techniques is to employ a hybrid approach that combines low-resolution shape information about macromolecules and their complexes from experimental hydrodynamic (e.g. analytical ultracentrifugation) and solution scattering measurements (e.g., solution X-ray or neutron scattering), with computational modeling to obtain atomic-level models. While promising, scattering methods rely on aggregation-free, monodispersed preparations and therefore the careful development of a quality control pipeline is fundamental to an unbiased and reliable structural determination. This review article describes hydrodynamic techniques that are highly valuable for homogeneity studies, scattering techniques useful to study the low-resolution shape, and strategies for computational modeling to obtain high-resolution 3D structural models of RNAs, proteins, and RNA-protein complexes. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

Non-Destructive High-Resolution Organic Matter Record on Lake Sediment using Steady-State Solid Phase Fluorescence: Organic Matter Quality and Quantity Assessment.

NASA Astrophysics Data System (ADS)

Quiers, M.; Perrette, Y.; Etienne, D.; Develle, A. L.; Jacq, K.

2017-12-01

The use of organic proxies increases in paleoenvironmental reconstructions from natural archives. Major advances have been achieved by the development of new highly informative molecular proxies usually linked to specific compounds. While studies focused on targeted compounds, offering a high information degree, advances on bulk organic matter are limited. However, this bulk is the main contributor to carbon cycle and has been shown to be a driver of many mineral or organic compounds transfer and record. Development of target proxies need complementary information on bulk organic matter to understand biases link to controlling factors or analytical methods, and provide a robust interpretation. Fluorescence methods have often been employed to characterize and quantify organic matter. However, these technics are mainly developed for liquid samples, inducing material and resolution loss when working on natural archives (either stalagmite or sediments). High-resolution solid phase fluorescence (SPF) was developed on speleothems. This method allows now to analyse organic matter quality and quantity if procedure to constrain the optical density are adopted. In fact, a calibration method using liquid phase fluorescence (LPF) was developed for speleothem, allowing to quantify organic carbon at high-resolution. We report here an application of such a procedure SPF/LPF measurements on lake sediments. In order to avoid sediment matrix effects on the fluorescence signal, a calibration using LPF measurements was realised. First results using this method provided organic matter quality record of different organic matter compounds (humic-like, protein-like and chlorophylle-like compounds) at high resolution for the sediment core. High resolution organic matter fluxes are obtained in a second time, applying pragmatic chemometrics model (non linear models, partial least square models) on high resolution fluorescence data. SPF method can be considered as a promising tool for high resolution record on organic matter quality and quantity. Potential application of this method will be evocated (lake ecosystem dynamic, changes in trophic levels)

Non-invasive measurement of frog skin reflectivity in high spatial resolution using a dual hyperspectral approach.

PubMed

Pinto, Francisco; Mielewczik, Michael; Liebisch, Frank; Walter, Achim; Greven, Hartmut; Rascher, Uwe

2013-01-01

Most spectral data for the amphibian integument are limited to the visible spectrum of light and have been collected using point measurements with low spatial resolution. In the present study a dual camera setup consisting of two push broom hyperspectral imaging systems was employed, which produces reflectance images between 400 and 2500 nm with high spectral and spatial resolution and a high dynamic range. We briefly introduce the system and document the high efficiency of this technique analyzing exemplarily the spectral reflectivity of the integument of three arboreal anuran species (Litoria caerulea, Agalychnis callidryas and Hyla arborea), all of which appear green to the human eye. The imaging setup generates a high number of spectral bands within seconds and allows non-invasive characterization of spectral characteristics with relatively high working distance. Despite the comparatively uniform coloration, spectral reflectivity between 700 and 1100 nm differed markedly among the species. In contrast to H. arborea, L. caerulea and A. callidryas showed reflection in this range. For all three species, reflectivity above 1100 nm is primarily defined by water absorption. Furthermore, the high resolution allowed examining even small structures such as fingers and toes, which in A. callidryas showed an increased reflectivity in the near infrared part of the spectrum. Hyperspectral imaging was found to be a very useful alternative technique combining the spectral resolution of spectrometric measurements with a higher spatial resolution. In addition, we used Digital Infrared/Red-Edge Photography as new simple method to roughly determine the near infrared reflectivity of frog specimens in field, where hyperspectral imaging is typically difficult.

Comparing High Definition Live Interactive and Store-and-Forward Consultations to In-Person Examinations.

PubMed

Marchell, Richard; Locatis, Craig; Burges, Gene; Maisiak, Richard; Liu, Wei-Li; Ackerman, Michael

2017-03-01

There is little teledermatology research directly comparing remote methods, even less research with two in-person dermatologist agreement providing a baseline for comparing remote methods, and no research using high definition video as a live interactive method. To compare in-person consultations with store-and-forward and live interactive methods, the latter having two levels of image quality. A controlled study was conducted where patients were examined in-person, by high definition video, and by store-and-forward methods. The order patients experienced methods and residents assigned methods rotated, although an attending always saw patients in-person. The type of high definition video employed, lower resolution compressed or higher resolution uncompressed, was alternated between clinics. Primary and differential diagnoses, biopsy recommendations, and diagnostic and biopsy confidence ratings were recorded. Concordance and confidence were significantly better for in-person versus remote methods and biopsy recommendations were lower. Store-and-forward and higher resolution uncompressed video results were similar and better than those for lower resolution compressed video. Dermatology residents took store-and-forward photos and their quality was likely superior to those normally taken in practice. There were variations in expertise between the attending and second and third year residents. The superiority of in-person consultations suggests the tendencies to order more biopsies or still see patients in-person are often justified in teledermatology and that high resolution uncompressed video can close the resolution gap between store-and-forward and live interactive methods.

High resolution simulations on the North Aegean Sea seasonal circulation

NASA Astrophysics Data System (ADS)

Kourafalou, V. H.; Barbopoulos, K.

2003-01-01

The seasonal characteristics of the circulation in the North Aegean Sea are examined with the aid of a climatological type simulation (three-year run with perpetual year forcing) on a fine resolution grid (2.5 km by 2.5 km). The model is based on the Princeton Ocean Model with a parameterisation of plume dynamics that is employed for the input of waters with hydrographic properties that are different than the properties of basin waters, as the Black Sea Water (BSW) outflow through the Dardanelles Strait and riverine sources. The model is nested with a sequence of coarser regional and basin-wide models that provide for the long-term interaction between the study area and the Eastern Mediterranean at large. The results are employed to discuss the response of the North Aegean to the important circulation forcing mechanisms in the region, namely wind stress, heat and salt fluxes, buoyancy due to rivers and the BSW outflow (which is low in salinity and occasionally low in temperature) and the interaction with the Southern Aegean. The high resolution allows for the detailed representation of the complicated topography that presides in the region. This helps produce a rich eddy field and it allows for variability in the pathways of BSW that has implications in the basin hydrography and circulation.

Rapid quantitative chemical mapping of surfaces with sub-2 nm resolution

NASA Astrophysics Data System (ADS)

Lai, Chia-Yun; Perri, Saverio; Santos, Sergio; Garcia, Ricardo; Chiesa, Matteo

2016-05-01

We present a theory that exploits four observables in bimodal atomic force microscopy to produce maps of the Hamaker constant H. The quantitative H maps may be employed by the broader community to directly interpret the high resolution of standard bimodal AFM images as chemical maps while simultaneously quantifying chemistry in the non-contact regime. We further provide a simple methodology to optimize a range of operational parameters for which H is in the closest agreement with the Lifshitz theory in order to (1) simplify data acquisition and (2) generalize the methodology to any set of cantilever-sample systems.We present a theory that exploits four observables in bimodal atomic force microscopy to produce maps of the Hamaker constant H. The quantitative H maps may be employed by the broader community to directly interpret the high resolution of standard bimodal AFM images as chemical maps while simultaneously quantifying chemistry in the non-contact regime. We further provide a simple methodology to optimize a range of operational parameters for which H is in the closest agreement with the Lifshitz theory in order to (1) simplify data acquisition and (2) generalize the methodology to any set of cantilever-sample systems. Electronic supplementary information (ESI) available. See DOI: 10.1039/c6nr00496b

The planetary hydraulics analysis based on a multi-resolution stereo DTMs and LISFLOOD-FP model: Case study in Mars

NASA Astrophysics Data System (ADS)

Kim, J.; Schumann, G.; Neal, J. C.; Lin, S.

2013-12-01

Earth is the only planet possessing an active hydrological system based on H2O circulation. However, after Mariner 9 discovered fluvial channels on Mars with similar features to Earth, it became clear that some solid planets and satellites once had water flows or pseudo hydrological systems of other liquids. After liquid water was identified as the agent of ancient martian fluvial activities, the valley and channels on the martian surface were investigated by a number of remote sensing and in-suit measurements. Among all available data sets, the stereo DTM and ortho from various successful orbital sensor, such as High Resolution Stereo Camera (HRSC), Context Camera (CTX), and High Resolution Imaging Science Experiment (HiRISE), are being most widely used to trace the origin and consequences of martian hydrological channels. However, geomorphological analysis, with stereo DTM and ortho images over fluvial areas, has some limitations, and so a quantitative modeling method utilizing various spatial resolution DTMs is required. Thus in this study we tested the application of hydraulics analysis with multi-resolution martian DTMs, constructed in line with Kim and Muller's (2009) approach. An advanced LISFLOOD-FP model (Bates et al., 2010), which simulates in-channel dynamic wave behavior by solving 2D shallow water equations without advection, was introduced to conduct a high accuracy simulation together with 150-1.2m DTMs over test sites including Athabasca and Bahram valles. For application to a martian surface, technically the acceleration of gravity in LISFLOOD-FP was reduced to the martian value of 3.71 m s-2 and the Manning's n value (friction), the only free parameter in the model, was adjusted for martian gravity by scaling it. The approach employing multi-resolution stereo DTMs and LISFLOOD-FP was superior compared with the other research cases using a single DTM source for hydraulics analysis. HRSC DTMs, covering 50-150m resolutions was used to trace rough routes of water flows for extensive target areas. After then, refinements through hydraulics simulations with CTX DTMs (~12-18m resolution) and HiRISE DTMs (~1- 4m resolution) were conducted by employing the output of HRSC simulations as the initial conditions. Thus even a few high and very high resolution stereo DTMs coverage enabled the performance of a high precision hydraulics analysis for reconstructing a whole fluvial event. In this manner, useful information to identify the characteristics of martian fluvial activities, such as water depth along the time line, flow direction, and travel time, were successfully retrieved with each target tributary. Together with all above useful outputs of hydraulics analysis, the local roughness and photogrammetric control of the stereo DTMs appeared to be crucial elements for accurate fluvial simulation. The potential of this study should be further explored for its application to the other extraterrestrial bodies where fluvial activity once existed, as well as the major martian channel and valleys.

Estimating Soil Moisture at High Spatial Resolution with Three Radiometric Satellite Products: A Study from a South-Eastern Australian Catchment

NASA Astrophysics Data System (ADS)

Senanayake, I. P.; Yeo, I. Y.; Tangdamrongsub, N.; Willgoose, G. R.; Hancock, G. R.; Wells, T.; Fang, B.; Lakshmi, V.

2017-12-01

Long-term soil moisture datasets at high spatial resolution are important in agricultural, hydrological, and climatic applications. The soil moisture estimates can be achieved using satellite remote sensing observations. However, the satellite soil moisture data are typically available at coarse spatial resolutions ( several tens of km), therefore require further downscaling. Different satellite soil moisture products have to be conjointly employed in developing a consistent time-series of high resolution soil moisture, while the discrepancies amongst different satellite retrievals need to be resolved. This study aims to downscale three different satellite soil moisture products, the Soil Moisture and Ocean Salinity (SMOS, 25 km), the Soil Moisture Active Passive (SMAP, 36 km) and the SMAP-Enhanced (9 km), and to conduct an inter-comparison of the downscaled results. The downscaling approach is developed based on the relationship between the diurnal temperature difference and the daily mean soil moisture content. The approach is applied to two sub-catchments (Krui and Merriwa River) of the Goulburn River catchment in the Upper Hunter region (NSW, Australia) to estimate soil moisture at 1 km resolution for 2015. The three coarse spatial resolution soil moisture products and their downscaled results will be validated with the in-situ observations obtained from the Scaling and Assimilation of Soil Moisture and Streamflow (SASMAS) network. The spatial and temporal patterns of the downscaled results will also be analysed. This study will provide the necessary insights for data selection and bias corrections to maintain the consistency of a long-term high resolution soil moisture dataset. The results will assist in developing a time-series of high resolution soil moisture data over the south-eastern Australia.

Effects of Drake Passage on a strongly eddying global ocean

NASA Astrophysics Data System (ADS)

Viebahn, Jan; von der Heydt, Anna S.; Dijkstra, Henk A.

2015-04-01

During the past 65 Million (Ma) years, Earth's climate has undergone a major change from warm 'greenhouse' to colder 'icehouse' conditions with extensive ice sheets in the polar regions of both hemispheres. The Eocene-Oligocene (~34 Ma) and Oligocene-Miocene (~23 Ma) boundaries reflect major transitions in Cenozoic global climate change. Proposed mechanisms of these transitions include reorganization of ocean circulation due to critical gateway opening/deepening, changes in atmospheric CO2-concentration, and feedback mechanisms related to land-ice formation. Drake Passage (DP) is an intensively studied gateway because it plays a central role in closing the transport pathways of heat and chemicals in the ocean. The climate response to a closed DP has been explored with a variety of general circulation models, however, all of these models employ low model-grid resolutions such that the effects of subgrid-scale fluctuations ('eddies') are parameterized. We present results of the first high-resolution (0.1° horizontally) realistic global ocean model simulation with a closed DP in which the eddy field is largely resolved. The simulation extends over more than 200 years such that the strong transient adjustment process is passed and a near-equilibrium ocean state is reached. The effects of DP are diagnosed by comparing with both an open DP high-resolution control simulation (of same length) and corresponding low-resolution simulations. By focussing on the heat/tracer transports we demonstrate that the results are twofold: Considering spatially integrated transports the overall response to a closed DP is well captured by low-resolution simulations. However, looking at the actual spatial distributions drastic differences appear between far-scattered high-resolution and laminar-uniform low-resolution fields. We conclude that sparse and highly localized tracer proxy observations have to be interpreted carefully with the help of high-resolution model simulations.

Example-based super-resolution for single-image analysis from the Chang'e-1 Mission

NASA Astrophysics Data System (ADS)

Wu, Fan-Lu; Wang, Xiang-Jun

2016-11-01

Due to the low spatial resolution of images taken from the Chang'e-1 (CE-1) orbiter, the details of the lunar surface are blurred and lost. Considering the limited spatial resolution of image data obtained by a CCD camera on CE-1, an example-based super-resolution (SR) algorithm is employed to obtain high-resolution (HR) images. SR reconstruction is important for the application of image data to increase the resolution of images. In this article, a novel example-based algorithm is proposed to implement SR reconstruction by single-image analysis, and the computational cost is reduced compared to other example-based SR methods. The results show that this method can enhance the resolution of images using SR and recover detailed information about the lunar surface. Thus it can be used for surveying HR terrain and geological features. Moreover, the algorithm is significant for the HR processing of remotely sensed images obtained by other imaging systems.

High Frequency High Spectral Resolution Focal Plane Arrays for AtLAST

NASA Astrophysics Data System (ADS)

Baryshev, Andrey

2018-01-01

Large collecting area single dish telescope such as ATLAST will be especially effective for medium (R 1000) and high (R 50000) spectral resolution observations. Large focal plane array is a natural solution to increase mapping speed. For medium resolution direct detectors with filter banks (KIDs) and or heterodyne technology can be employed. We will analyze performance limits of comparable KID and SIS focal plane array taking into account quantum limit and high background condition of terrestrial observing site. For large heterodyne focal plane arrays, a high current density AlN junctions open possibility of large instantaneous bandwidth >40%. This and possible multi frequency band FPSs presents a practical challenge for spatial sampling and scanning strategies. We will discuss phase array feeds as a possible solution, including a modular back-end system, which can be shared between KID and SIS based FPA. Finally we will discuss achievable sensitivities and pixel co unts for a high frequency (>500 GHz) FPAs and address main technical challenges: LO distribution, wire counts, bias line multiplexing, and monolithic vs. discrete mixer component integration.

Multimodal hard x-ray imaging with resolution approaching 10 nm for studies in material science

NASA Astrophysics Data System (ADS)

Yan, Hanfei; Bouet, Nathalie; Zhou, Juan; Huang, Xiaojing; Nazaretski, Evgeny; Xu, Weihe; Cocco, Alex P.; Chiu, Wilson K. S.; Brinkman, Kyle S.; Chu, Yong S.

2018-03-01

We report multimodal scanning hard x-ray imaging with spatial resolution approaching 10 nm and its application to contemporary studies in the field of material science. The high spatial resolution is achieved by focusing hard x-rays with two crossed multilayer Laue lenses and raster-scanning a sample with respect to the nanofocusing optics. Various techniques are used to characterize and verify the achieved focus size and imaging resolution. The multimodal imaging is realized by utilizing simultaneously absorption-, phase-, and fluorescence-contrast mechanisms. The combination of high spatial resolution and multimodal imaging enables a comprehensive study of a sample on a very fine length scale. In this work, the unique multimodal imaging capability was used to investigate a mixed ionic-electronic conducting ceramic-based membrane material employed in solid oxide fuel cells and membrane separations (compound of Ce0.8Gd0.2O2‑x and CoFe2O4) which revealed the existence of an emergent material phase and quantified the chemical complexity at the nanoscale.

Polymerization shrinkage of a dental resin composite determined by a fiber optic Fizeau interferometer

NASA Astrophysics Data System (ADS)

Arenas, Gustavo; Noriega, Sergio; Vallo, Claudia; Duchowicz, Ricardo

2007-03-01

A fiber optic sensing method based on a Fizeau-type interferometric scheme was employed for monitoring linear polymerization shrinkage in dental restoratives. This technique offers several advantages over the conventional methods of measuring polymerization contraction. This simple, compact, non-invasive and self-calibrating system competes with both conventional and other high-resolution bulk interferometric techniques. In this work, an analysis of the quality of interference signal and fringes visibility was performed in order to characterize their resolution and application range. The measurements of percent linear contraction as a function of the sample thickness were carried out in this study on two dental composites: Filtek P60 (3M ESPE) Posterior Restorer and Filtek Z250 (3M ESPE) Universal Restorer. The results were discussed with respect to others obtained employing alternative techniques.

Application of the high resolution return beam vidicon

NASA Technical Reports Server (NTRS)

Cantella, M. J.

1977-01-01

The Return Beam Vidicon (RBV) is a high-performance electronic image sensor and electrical storage component. It can accept continuous or discrete exposures. Information can be read out with a single scan or with many repetitive scans for either signal processing or display. Resolution capability is 10,000 TV lines/height, and at 100 lp/mm, performance matches or exceeds that of film, particularly with low-contrast imagery. Electronic zoom can be employed effectively for image magnification and data compression. The high performance and flexibility of the RBV permit wide application in systems for reconnaissance, scan conversion, information storage and retrieval, and automatic inspection and test. This paper summarizes the characteristics and performance parameters of the RBV and cites examples of feasible applications.

VizieR Online Data Catalog: Abundance ratio for 5 local stellar associations (Reddy+, 2015)

NASA Astrophysics Data System (ADS)

Reddy, A. B. S.; Lambert, D. L.

2018-01-01

In this paper, we have performed a homogeneous and a comprehensive abundance analysis using high-resolution spectroscopy. High-resolution and high signal-to-noise (S/N) spectra of the program stars were obtained during the nights of 2015 February 10-11 with the Robert G. Tull coude cross-dispersed echelle spectrograph (Tull et al. 1995PASP..107..251T) of the 2.7 m Harlan J. Smith reflector at the McDonald Observatory. We employed a Tektronix 2048x2048 24 μm pixel, backside illuminated and anti-reflection coated CCD as a detector and an R2 echelle grating with 52.67 grooves/mm with exposures centred at 5060 Å. (7 data files).

Subtle trisomy 12q24.3 and subtle monosomy 22q13.3: three new cases and review.

PubMed

Rodríguez, Laura; Martínez Guardia, Nieves; Herens, Christian; Jamar, Mauricette; Verloes, Alain; López, Fermina; Santos Muñoz, José; Martínez-Frías, María Luisa

2003-10-01

The high resolution G-bands (850 bands) karyotype have made it possible to identify small chromosome anomalies (5 megabases) which are now microscopically visible. New techniques have been improved, such as the Fluorescent in situ hybridization (FISH) with subtelomeric probes, which can be employed to detect cryptic chromosome alterations not visible microscopically. We present three cases which had been remitted for a high resolution karyotype. The high resolution G-band karyotype and the FISH techniques led us to conclude that the three cases were carriers of a similar subtle chromosomal alteration. Case I is a new born female with developmental and psychomotor delay, hypotonia, and long limbs with arachnodactily. A high resolution G-band karyotype showed an abnormal chromosome 22. FISH techniques confirmed a der(22)t(12;22)(q24.31;q13.3). Case II is a 12-year-old girl, with growth retardation, long shaped face with thick eyebrows, smooth philtrum, and thin upper lip with severe mental retardation (still no language), with a phenotype very similar to that of his sister: long shaped face, thick eyebrows, smooth philtrum, and thin upper lip. A high resolution G-band karyotype also showed in Case II and III an abnormal chromosome 22, studied by FISH techniques which confirmed a der(22)t(12;22)(q24.3;q13.3) in both cases. Copyright 2003 Wiley-Liss, Inc.

A dynamic aerodynamic resistance approach to calculate high resolution sensible heat fluxes in urban areas

NASA Astrophysics Data System (ADS)

Crawford, Ben; Grimmond, Sue; Kent, Christoph; Gabey, Andrew; Ward, Helen; Sun, Ting; Morrison, William

2017-04-01

Remotely sensed data from satellites have potential to enable high-resolution, automated calculation of urban surface energy balance terms and inform decisions about urban adaptations to environmental change. However, aerodynamic resistance methods to estimate sensible heat flux (QH) in cities using satellite-derived observations of surface temperature are difficult in part due to spatial and temporal variability of the thermal aerodynamic resistance term (rah). In this work, we extend an empirical function to estimate rah using observational data from several cities with a broad range of surface vegetation land cover properties. We then use this function to calculate spatially and temporally variable rah in London based on high-resolution (100 m) land cover datasets and in situ meteorological observations. In order to calculate high-resolution QH based on satellite-observed land surface temperatures, we also develop and employ novel methods to i) apply source area-weighted averaging of surface and meteorological variables across the study spatial domain, ii) calculate spatially variable, high-resolution meteorological variables (wind speed, friction velocity, and Obukhov length), iii) incorporate spatially interpolated urban air temperatures from a distributed sensor network, and iv) apply a modified Monte Carlo approach to assess uncertainties with our results, methods, and input variables. Modeled QH using the aerodynamic resistance method is then compared to in situ observations in central London from a unique network of scintillometers and eddy-covariance measurements.

Classification of Microcalcifications for the Diagnosis of Breast Cancer Using Artificial Neural Networks.

DTIC Science & Technology

1997-09-01

employed to classify benign and malignant microcalcifications in the radiographs of pathological specimen that were digitized at a high resolution of...21 microns x 21 microns. The CNN achieved an Az value of 0.90 in classifying clusters of microcalcifications associated with benign and malignant processes

Edge-following algorithm for tracking geological features

NASA Technical Reports Server (NTRS)

Tietz, J. C.

1977-01-01

Sequential edge-tracking algorithm employs circular scanning to point permit effective real-time tracking of coastlines and rivers from earth resources satellites. Technique eliminates expensive high-resolution cameras. System might also be adaptable for application in monitoring automated assembly lines, inspecting conveyor belts, or analyzing thermographs, or x ray images.

Tablet disintegration studied by high-resolution real-time magnetic resonance imaging.

PubMed

Quodbach, Julian; Moussavi, Amir; Tammer, Roland; Frahm, Jens; Kleinebudde, Peter

2014-01-01

The present work employs recent advances in high-resolution real-time magnetic resonance imaging (MRI) to investigate the disintegration process of tablets containing disintegrants. A temporal resolution of 75 ms and a spatial resolution of 80 × 80 µm with a section thickness of only 600 µm were achieved. The histograms of MRI videos were quantitatively analyzed with MATLAB. The mechanisms of action of six commercially available disintegrants, the influence of relative tablet density, and the impact of disintegrant concentration were examined. Crospovidone seems to be the only disintegrant acting by a shape memory effect, whereas the others mainly swell. A higher relative density of tablets containing croscarmellose sodium leads to a more even distribution of water within the tablet matrix but hardly impacts the disintegration kinetics. Increasing the polacrilin potassium disintegrant concentration leads to a quicker and more thorough disintegration process. Real-time MRI emerges as valuable tool to visualize and investigate the process of tablet disintegration.

High-resolution mapping of forest carbon stocks in the Colombian Amazon

NASA Astrophysics Data System (ADS)

Asner, G. P.; Clark, J. K.; Mascaro, J.; Galindo García, G. A.; Chadwick, K. D.; Navarrete Encinales, D. A.; Paez-Acosta, G.; Cabrera Montenegro, E.; Kennedy-Bowdoin, T.; Duque, Á.; Balaji, A.; von Hildebrand, P.; Maatoug, L.; Bernal, J. F. Phillips; Yepes Quintero, A. P.; Knapp, D. E.; García Dávila, M. C.; Jacobson, J.; Ordóñez, M. F.

2012-07-01

High-resolution mapping of tropical forest carbon stocks can assist forest management and improve implementation of large-scale carbon retention and enhancement programs. Previous high-resolution approaches have relied on field plot and/or light detection and ranging (LiDAR) samples of aboveground carbon density, which are typically upscaled to larger geographic areas using stratification maps. Such efforts often rely on detailed vegetation maps to stratify the region for sampling, but existing tropical forest maps are often too coarse and field plots too sparse for high-resolution carbon assessments. We developed a top-down approach for high-resolution carbon mapping in a 16.5 million ha region (> 40%) of the Colombian Amazon - a remote landscape seldom documented. We report on three advances for large-scale carbon mapping: (i) employing a universal approach to airborne LiDAR-calibration with limited field data; (ii) quantifying environmental controls over carbon densities; and (iii) developing stratification- and regression-based approaches for scaling up to regions outside of LiDAR coverage. We found that carbon stocks are predicted by a combination of satellite-derived elevation, fractional canopy cover and terrain ruggedness, allowing upscaling of the LiDAR samples to the full 16.5 million ha region. LiDAR-derived carbon maps have 14% uncertainty at 1 ha resolution, and the regional map based on stratification has 28% uncertainty in any given hectare. High-resolution approaches with quantifiable pixel-scale uncertainties will provide the most confidence for monitoring changes in tropical forest carbon stocks. Improved confidence will allow resource managers and decision makers to more rapidly and effectively implement actions that better conserve and utilize forests in tropical regions.

High-resolution Mapping of Forest Carbon Stocks in the Colombian Amazon

NASA Astrophysics Data System (ADS)

Asner, G. P.; Clark, J. K.; Mascaro, J.; Galindo García, G. A.; Chadwick, K. D.; Navarrete Encinales, D. A.; Paez-Acosta, G.; Cabrera Montenegro, E.; Kennedy-Bowdoin, T.; Duque, Á.; Balaji, A.; von Hildebrand, P.; Maatoug, L.; Bernal, J. F. Phillips; Knapp, D. E.; García Dávila, M. C.; Jacobson, J.; Ordóñez, M. F.

2012-03-01

High-resolution mapping of tropical forest carbon stocks can assist forest management and improve implementation of large-scale carbon retention and enhancement programs. Previous high-resolution approaches have relied on field plot and/or Light Detection and Ranging (LiDAR) samples of aboveground carbon density, which are typically upscaled to larger geographic areas using stratification maps. Such efforts often rely on detailed vegetation maps to stratify the region for sampling, but existing tropical forest maps are often too coarse and field plots too sparse for high resolution carbon assessments. We developed a top-down approach for high-resolution carbon mapping in a 16.5 million ha region (>40 %) of the Colombian Amazon - a remote landscape seldom documented. We report on three advances for large-scale carbon mapping: (i) employing a universal approach to airborne LiDAR-calibration with limited field data; (ii) quantifying environmental controls over carbon densities; and (iii) developing stratification- and regression-based approaches for scaling up to regions outside of LiDAR coverage. We found that carbon stocks are predicted by a combination of satellite-derived elevation, fractional canopy cover and terrain ruggedness, allowing upscaling of the LiDAR samples to the full 16.5 million ha region. LiDAR-derived carbon mapping samples had 14.6 % uncertainty at 1 ha resolution, and regional maps based on stratification and regression approaches had 25.6 % and 29.6 % uncertainty, respectively, in any given hectare. High-resolution approaches with reported local-scale uncertainties will provide the most confidence for monitoring changes in tropical forest carbon stocks. Improved confidence will allow resource managers and decision-makers to more rapidly and effectively implement actions that better conserve and utilize forests in tropical regions.

Fusion and quality analysis for remote sensing images using contourlet transform

NASA Astrophysics Data System (ADS)

Choi, Yoonsuk; Sharifahmadian, Ershad; Latifi, Shahram

2013-05-01

Recent developments in remote sensing technologies have provided various images with high spatial and spectral resolutions. However, multispectral images have low spatial resolution and panchromatic images have low spectral resolution. Therefore, image fusion techniques are necessary to improve the spatial resolution of spectral images by injecting spatial details of high-resolution panchromatic images. The objective of image fusion is to provide useful information by improving the spatial resolution and the spectral information of the original images. The fusion results can be utilized in various applications, such as military, medical imaging, and remote sensing. This paper addresses two issues in image fusion: i) image fusion method and ii) quality analysis of fusion results. First, a new contourlet-based image fusion method is presented, which is an improvement over the wavelet-based fusion. This fusion method is then applied to a case study to demonstrate its fusion performance. Fusion framework and scheme used in the study are discussed in detail. Second, quality analysis for the fusion results is discussed. We employed various quality metrics in order to analyze the fusion results both spatially and spectrally. Our results indicate that the proposed contourlet-based fusion method performs better than the conventional wavelet-based fusion methods.

Oxygen Measurements in Liposome Encapsulated Hemoglobin

NASA Astrophysics Data System (ADS)

Phiri, Joshua Benjamin

Liposome encapsulated hemoglobins (LEH's) are of current interest as blood substitutes. An analytical methodology for rapid non-invasive measurements of oxygen in artificial oxygen carriers is examined. High resolution optical absorption spectra are calculated by means of a one dimensional diffusion approximation. The encapsulated hemoglobin is prepared from fresh defibrinated bovine blood. Liposomes are prepared from hydrogenated soy phosphatidylcholine (HSPC), cholesterol and dicetylphosphate using a bath sonication method. An integrating sphere spectrophotometer is employed for diffuse optics measurements. Data is collected using an automated data acquisition system employing lock-in -amplifiers. The concentrations of hemoglobin derivatives are evaluated from the corresponding extinction coefficients using a numerical technique of singular value decomposition, and verification of the results is done using Monte Carlo simulations. In situ measurements are required for the determination of hemoglobin derivatives because most encapsulation methods invariably lead to the formation of methemoglobin, a nonfunctional form of hemoglobin. The methods employed in this work lead to high resolution absorption spectra of oxyhemoglobin and other derivatives in red blood cells and liposome encapsulated hemoglobin (LEH). The analysis using singular value decomposition method offers a quantitative means of calculating the fractions of oxyhemoglobin and other hemoglobin derivatives in LEH samples. The analytical methods developed in this work will become even more useful when production of LEH as a blood substitute is scaled up to large volumes.

29 CFR 1603.108 - Settlement and alternative dispute resolution.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 4 2013-07-01 2013-07-01 false Settlement and alternative dispute resolution. 1603.108 Section 1603.108 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION...

29 CFR 1603.108 - Settlement and alternative dispute resolution.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 4 2014-07-01 2014-07-01 false Settlement and alternative dispute resolution. 1603.108 Section 1603.108 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION...

29 CFR 1603.108 - Settlement and alternative dispute resolution.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 4 2012-07-01 2012-07-01 false Settlement and alternative dispute resolution. 1603.108 Section 1603.108 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION...

29 CFR 1603.108 - Settlement and alternative dispute resolution.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 4 2010-07-01 2010-07-01 false Settlement and alternative dispute resolution. 1603.108 Section 1603.108 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION...

29 CFR 1603.108 - Settlement and alternative dispute resolution.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 4 2011-07-01 2011-07-01 false Settlement and alternative dispute resolution. 1603.108 Section 1603.108 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION...

Determination of the Isotopic Enrichment of 13C- and 2H-Labeled Tracers of Glucose Using High-Resolution Mass Spectrometry: Application to Dual- and Triple-Tracer Studies.

PubMed

Trötzmüller, Martin; Triebl, Alexander; Ajsic, Amra; Hartler, Jürgen; Köfeler, Harald; Regittnig, Werner

2017-11-21

Multiple-tracer approaches for investigating glucose metabolism in humans usually involve the administration of stable and radioactive glucose tracers and the subsequent determination of tracer enrichments in sampled blood. When using conventional, low-resolution mass spectrometry (LRMS), the number of spectral interferences rises rapidly with the number of stable tracers employed. Thus, in LRMS, both computational effort and statistical uncertainties associated with the correction for spectral interferences limit the number of stable tracers that can be simultaneously employed (usually two). Here we show that these limitations can be overcome by applying high-resolution mass spectrometry (HRMS). The HRMS method presented is based on the use of an Orbitrap mass spectrometer operated at a mass resolution of 100 000 to allow electrospray-generated ions of the deprotonated glucose molecules to be monitored at their exact masses. The tracer enrichment determination in blood plasma is demonstrated for several triple combinations of 13 C- and 2 H-labeled glucose tracers (e.g., [1- 2 H 1 ]-, [6,6- 2 H 2 ]-, [1,6- 13 C 2 ]glucose). For each combination it is shown that ions arising from 2 H-labeled tracers are completely differentiated from those arising from 13 C-labeled tracers, thereby allowing the enrichment of a tracer to be simply calculated from the observed ion intensities using a standard curve with curve parameters unaffected by the presence of other tracers. For each tracer, the HRMS method exhibits low limits of detection and good repeatability in the tested 0.1-15.0% enrichment range. Additionally, due to short sample preparation and analysis times, the method is well-suited for high-throughput determination of multiple glucose tracer enrichments in plasma samples.

Numerical simulation of immiscible viscous fingering using adaptive unstructured meshes

NASA Astrophysics Data System (ADS)

Adam, A.; Salinas, P.; Percival, J. R.; Pavlidis, D.; Pain, C.; Muggeridge, A. H.; Jackson, M.

2015-12-01

Displacement of one fluid by another in porous media occurs in various settings including hydrocarbon recovery, CO2 storage and water purification. When the invading fluid is of lower viscosity than the resident fluid, the displacement front is subject to a Saffman-Taylor instability and is unstable to transverse perturbations. These instabilities can grow, leading to fingering of the invading fluid. Numerical simulation of viscous fingering is challenging. The physics is controlled by a complex interplay of viscous and diffusive forces and it is necessary to ensure physical diffusion dominates numerical diffusion to obtain converged solutions. This typically requires the use of high mesh resolution and high order numerical methods. This is computationally expensive. We demonstrate here the use of a novel control volume - finite element (CVFE) method along with dynamic unstructured mesh adaptivity to simulate viscous fingering with higher accuracy and lower computational cost than conventional methods. Our CVFE method employs a discontinuous representation for both pressure and velocity, allowing the use of smaller control volumes (CVs). This yields higher resolution of the saturation field which is represented CV-wise. Moreover, dynamic mesh adaptivity allows high mesh resolution to be employed where it is required to resolve the fingers and lower resolution elsewhere. We use our results to re-examine the existing criteria that have been proposed to govern the onset of instability.Mesh adaptivity requires the mapping of data from one mesh to another. Conventional methods such as consistent interpolation do not readily generalise to discontinuous fields and are non-conservative. We further contribute a general framework for interpolation of CV fields by Galerkin projection. The method is conservative, higher order and yields improved results, particularly with higher order or discontinuous elements where existing approaches are often excessively diffusive.

Advances of a Brillouin Scattering Lidar System for the Detection of Temperature Profiles in the Ocean: Laboratory Measurements and Field Test

NASA Astrophysics Data System (ADS)

Walther, T.; Rupp, D.; Friman, S.; Trees, C.; Fournier, G.

2016-02-01

Recently we have demonstrated the feasibility of remotely measuring temperature profiles in water under a laboratory environment employing our real-time Brillouin Scattering LIDAR (BSL) system. The working principle is based on the frequency and time resolved detection of the backscattered spontaneous Brillouin signal of a short light pulse fired into the ocean. The light source consists of a frequency-doubled fiber-amplified External Cavity Diode Laser (ECDL) providing high-energy, Fourier transform-limited laser pulses in the green spectral range. The Brillouin shift is detected with high accuracy (low uncertainty) by employing an edge filter based on an Excited State Faraday Anomalous Dispersion Optical Filter (ESFADOF). Time-resolution allows for the depth resolution and the frequency resolved shift is proportional to the speed of sound. Thus, the temperature profile can be extracted from the measurements. In our laboratory setup we were able to resolve water temperatures with a mean accuracy of up to 0.07 oC and a spatial resolution of 1 m depending on the amount of averaging. In order to prepare the system for a first field test under realistic conditions on the coast of the Mediterranean at CMRE in La Spezia, almost all of the components have been upgraded. This first test is planned for November 2015. We will present the above mentioned measurements, details about the upgrades and report on our experiences during this maritime field test.Ultimately, the plan is to operate the system from a mobile platform, e.g., a helicopter or vessel, in order to precisely determine the temperature of the surface mixed layer of the ocean with high spatial resolution.

TH-AB-BRA-01: A Novel Doubly-Focused Multileaf Collimator Design for MR-Guided Radiation Therapy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, H; Mutic, S; Green, O

2016-06-15

Purpose: To describe the physical and dosimetric properties of a novel double-stack multileaf collimator (MLC). Methods: One of the compromises made in the MLC design has been to employ linear-motion singly-divergent shapes. Because the MLC leading edge moves linearly, it is rounded to provide a consistent, albeit compromised penumbra. The MLC employed in the new linac-based MR-IGRT unit is designed to be doubly focused in that each leaf moves in an arc centered at the source, and the sides of the leaves are machined such that they lie parallel to a line between the leaf edge and the source. Themore » curvature of the MLC keeps motors and encoders in lower magnetic field. However, high spatial-resolution leaves are difficult to manufacture to sufficiently tight tolerances and difficult to move due to restricted space on the gantry. Wider leaves alleviate this problem with less moving parts but the coarse resolution disallows treating very small lesions. This compromise has been overcome by splitting the MLC leaf bank into two sets, stacked one upon the other and offset half of a leaf width. The dosimetry has been simulated using Monte-Carlo and a 6 MV linac in a 0.35 T magnetic field. Results: The combined MLC leaf set has a spatial resolution of effectively half of the leaf width, 4mm here. The dosimetry resolution and conformality are consistent with 4mm wide MLC assisted by inverse fluence modulation. Also, because each leaf junction is backed up by the stacked leaf that lies over the junction, the problem of tongue-and-groove dosimetry has been greatly reduced. The novel MLC design allows the use of more powerful leaf motors than would be otherwise possible if a single MLC bank is employed. Conclusions: The stacked MLC will provide highly conformal dose distributions suitable for stereotactic radiation therapy of small lesions. The research was funded by ViewRay, Inc.« less

Data compression techniques applied to high resolution high frame rate video technology

NASA Technical Reports Server (NTRS)

Hartz, William G.; Alexovich, Robert E.; Neustadter, Marc S.

1989-01-01

An investigation is presented of video data compression applied to microgravity space experiments using High Resolution High Frame Rate Video Technology (HHVT). An extensive survey of methods of video data compression, described in the open literature, was conducted. The survey examines compression methods employing digital computing. The results of the survey are presented. They include a description of each method and assessment of image degradation and video data parameters. An assessment is made of present and near term future technology for implementation of video data compression in high speed imaging system. Results of the assessment are discussed and summarized. The results of a study of a baseline HHVT video system, and approaches for implementation of video data compression, are presented. Case studies of three microgravity experiments are presented and specific compression techniques and implementations are recommended.

Plans for a new rio-imager experiment in Northern Scandinavia

NASA Astrophysics Data System (ADS)

Nielsen, E.; Hagfors, T.

1997-05-01

To observe the spatial variations and dynamics of charged particle precipitation in the high latitude ionosphere, a riometer experiment is planned, which from the ground will image the precipitation regions over an area of 300 × 300 km with a spatial resolution of 6 km in the zenith, increasing to 12 km at 60° zenith angle. The time resolution is one second. The spatial resolution represents a considerable improvement over existing imaging systems. The experiment employs a Mill's Cross technique not used before in riometer work: two 32 element rows of antennas form the antenna array, two 32 element Butler Matrices achieve directionality, and cross-correlation yield the directional intensities.

Subranging scheme for SQUID sensors

NASA Technical Reports Server (NTRS)

Penanen, Konstantin I. (Inventor)

2008-01-01

A readout scheme for measuring the output from a SQUID-based sensor-array using an improved subranging architecture that includes multiple resolution channels (such as a coarse resolution channel and a fine resolution channel). The scheme employs a flux sensing circuit with a sensing coil connected in series to multiple input coils, each input coil being coupled to a corresponding SQUID detection circuit having a high-resolution SQUID device with independent linearizing feedback. A two-resolution configuration (course and fine) is illustrated with a primary SQUID detection circuit for generating a fine readout, and a secondary SQUID detection circuit for generating a course readout, both having feedback current coupled to the respective SQUID devices via feedback/modulation coils. The primary and secondary SQUID detection circuits function and derive independent feedback. Thus, the SQUID devices may be monitored independently of each other (and read simultaneously) to dramatically increase slew rates and dynamic range.

Mass Spectrometric Imaging Using Laser Ablation and Solvent Capture by Aspiration (LASCA)

NASA Astrophysics Data System (ADS)

Brauer, Jonathan I.; Beech, Iwona B.; Sunner, Jan

2015-09-01

A novel interface for ambient, laser ablation-based mass spectrometric imaging (MSI) referred to as laser ablation and solvent capture by aspiration (LASCA) is presented and its performance demonstrated using selected, unaltered biological materials. LASCA employs a pulsed 2.94 μm laser beam for specimen ablation. Ablated materials in the laser plumes are collected on a hanging solvent droplet with electric field-enhanced trapping, followed by aspiration of droplets and remaining plume material in the form of a coarse aerosol into a collection capillary. The gas and liquid phases are subsequently separated in a 10 μL-volume separatory funnel, and the solution is analyzed with electrospray ionization in a high mass resolution Q-ToF mass spectrometer. The LASCA system separates the sampling and ionization steps in MSI and combines high efficiencies of laser plume sampling and of electrospray ionization (ESI) with high mass resolution MS. Up to 2000 different compounds are detected from a single ablation spot (pixel). Using the LASCA platform, rapid (6 s per pixel), high sensitivity, high mass-resolution ambient imaging of "as-received" biological material is achieved routinely and reproducibly.

A high-resolution Fourier Transform Spectrometer for planetary spectroscopy

NASA Technical Reports Server (NTRS)

Cruikshank, D. P.; Sinton, W. M.

1973-01-01

The employment of a high-resolution Fourier Transform Spectrometer (FTS) is described for planetary and other astronomical spectroscopy in conjunction with the 88-inch telescope at Mauna Kea Observatory. The FTS system is designed for a broad range of uses, including double-beam laboratory spectroscopy, infrared gas chromatography, and nuclear magnetic resonance spectroscopy. The data system is well-suited to astronomical applications because of its great speed in acquiring and transforming data, and because of the enormous storage capability of the magnetic tape unit supplied with the system. The basic instrument is outlined 2nd some of the initial results from the first attempted use on the Mauna Kea 88-inch telescope are reported.

High resolution projection X-ray microscope equipped with fluorescent X-ray analyzer and its applications

NASA Astrophysics Data System (ADS)

Minami, K.; Saito, Y.; Kai, H.; Shirota, K.; Yada, K.

2009-09-01

We have newly developed an open type fine-focus X-ray tube "TX-510" to realize a spatial resolution of 50nm and to radiate low energy characteristic X-rays for giving high absorption contrast to images of microscopic organisms. The "TX-510" employs a ZrO/W(100) Schottky emitter and an "In-Lens Field Emission Gun". The key points of the improvements are (1) reduced spherical aberration coefficient of magnetic objective lens, (2) easy and accurate focusing, (3) newly designed astigmatism compensator, (4) segmented thin film target for interchanging the target materials by electron beam shift and (5) fluorescent X-ray analysis system.

Impact of four-dimensional data assimilation (FDDA) on urban climate analysis

NASA Astrophysics Data System (ADS)

Pan, Linlin; Liu, Yubao; Liu, Yuewei; Li, Lei; Jiang, Yin; Cheng, Will; Roux, Gregory

2015-12-01

This study investigates the impact of four-dimensional data assimilation (FDDA) on urban climate analysis, which employs the NCAR (National Center for Atmospheric Research) WRF (the weather research and forecasting model) based on climate FDDA (CFDDA) technology to develop an urban-scale microclimatology database for the Shenzhen area, a rapidly developing metropolitan located along the southern coast of China, where uniquely high-density observations, including ultrahigh-resolution surface AWS (automatic weather station) network, radio sounding, wind profilers, radiometers, and other weather observation platforms, have been installed. CFDDA is an innovative dynamical downscaling regional climate analysis system that assimilates diverse regional observations; and has been employed to produce a 5 year multiscale high-resolution microclimate analysis by assimilating high-density observations at Shenzhen area. The CFDDA system was configured with four nested-grid domains at grid sizes of 27, 9, 3, and 1 km, respectively. This research evaluates the impact of assimilating high-resolution observation data on reproducing the refining features of urban-scale circulations. Two experiments were conducted with a 5 year run using CFSR (climate forecast system reanalysis) as boundary and initial conditions: one with CFDDA and the other without. The comparisons of these two experiments with observations indicate that CFDDA greatly reduces the model analysis error and is able to realistically analyze the microscale features such as urban-rural-coastal circulation, land/sea breezes, and local-hilly terrain thermal circulations. It is demonstrated that the urbanization can produce 2.5 k differences in 2 m temperatures, delays/speeds up the land/sea breeze development, and interacts with local mountain-valley circulations.

Land surface sensitivity of monsoon depressions formed over Bay of Bengal using improved high-resolution land state

NASA Astrophysics Data System (ADS)

Rajesh, P. V.; Pattnaik, S.; Mohanty, U. C.; Rai, D.; Baisya, H.; Pandey, P. C.

2017-12-01

Monsoon depressions (MDs) constitute a large fraction of the total rainfall during the Indian summer monsoon season. In this study, the impact of high-resolution land state is addressed by assessing the evolution of inland moving depressions formed over the Bay of Bengal using a mesoscale modeling system. Improved land state is generated using High Resolution Land Data Assimilation System employing Noah-MP land-surface model. Verification of soil moisture using Soil Moisture and Ocean Salinity (SMOS) and soil temperature using tower observations demonstrate promising results. Incorporating high-resolution land state yielded least root mean squared errors with higher correlation coefficient in the surface and mid tropospheric parameters. Rainfall forecasts reveal that simulations are spatially and quantitatively in accordance with observations and provide better skill scores. The improved land surface characteristics have brought about the realistic evolution of surface, mid-tropospheric parameters, vorticity and moist static energy that facilitates the accurate MDs dynamics in the model. Composite moisture budget analysis reveals that the surface evaporation is negligible compared to moisture flux convergence of water vapor, which supplies moisture into the MDs over land. The temporal relationship between rainfall and moisture convergence show high correlation, suggesting a realistic representation of land state help restructure the moisture inflow into the system through rainfall-moisture convergence feedback.

High resolution telescope

DOEpatents

Massie, Norbert A.; Oster, Yale

1992-01-01

A large effective-aperture, low-cost optical telescope with diffraction-limited resolution enables ground-based observation of near-earth space objects. The telescope has a non-redundant, thinned-aperture array in a center-mount, single-structure space frame. It employs speckle interferometric imaging to achieve diffraction-limited resolution. The signal-to-noise ratio problem is mitigated by moving the wavelength of operation to the near-IR, and the image is sensed by a Silicon CCD. The steerable, single-structure array presents a constant pupil. The center-mount, radar-like mount enables low-earth orbit space objects to be tracked as well as increases stiffness of the space frame. In the preferred embodiment, the array has elemental telescopes with subaperture of 2.1 m in a circle-of-nine configuration. The telescope array has an effective aperture of 12 m which provides a diffraction-limited resolution of 0.02 arc seconds. Pathlength matching of the telescope array is maintained by an electro-optical system employing laser metrology. Speckle imaging relaxes pathlength matching tolerance by one order of magnitude as compared to phased arrays. Many features of the telescope contribute to substantial reduction in costs. These include eliminating the conventional protective dome and reducing on-site construction activites. The cost of the telescope scales with the first power of the aperture rather than its third power as in conventional telescopes.

Estimation of Subpixel Snow-Covered Area by Nonparametric Regression Splines

NASA Astrophysics Data System (ADS)

Kuter, S.; Akyürek, Z.; Weber, G.-W.

2016-10-01

Measurement of the areal extent of snow cover with high accuracy plays an important role in hydrological and climate modeling. Remotely-sensed data acquired by earth-observing satellites offer great advantages for timely monitoring of snow cover. However, the main obstacle is the tradeoff between temporal and spatial resolution of satellite imageries. Soft or subpixel classification of low or moderate resolution satellite images is a preferred technique to overcome this problem. The most frequently employed snow cover fraction methods applied on Moderate Resolution Imaging Spectroradiometer (MODIS) data have evolved from spectral unmixing and empirical Normalized Difference Snow Index (NDSI) methods to latest machine learning-based artificial neural networks (ANNs). This study demonstrates the implementation of subpixel snow-covered area estimation based on the state-of-the-art nonparametric spline regression method, namely, Multivariate Adaptive Regression Splines (MARS). MARS models were trained by using MODIS top of atmospheric reflectance values of bands 1-7 as predictor variables. Reference percentage snow cover maps were generated from higher spatial resolution Landsat ETM+ binary snow cover maps. A multilayer feed-forward ANN with one hidden layer trained with backpropagation was also employed to estimate the percentage snow-covered area on the same data set. The results indicated that the developed MARS model performed better than th

High-resolution quantization based on soliton self-frequency shift and spectral compression in a bi-directional comb-fiber architecture

NASA Astrophysics Data System (ADS)

Zhang, Xuyan; Zhang, Zhiyao; Wang, Shubing; Liang, Dong; Li, Heping; Liu, Yong

2018-03-01

We propose and demonstrate an approach that can achieve high-resolution quantization by employing soliton self-frequency shift and spectral compression. Our approach is based on a bi-directional comb-fiber architecture which is composed of a Sagnac-loop-based mirror and a comb-like combination of N sections of interleaved single-mode fibers and high nonlinear fibers. The Sagnac-loop-based mirror placed at the terminal of a bus line reflects the optical pulses back to the bus line to achieve additional N-stage spectral compression, thus single-stage soliton self-frequency shift (SSFS) and (2 N - 1)-stage spectral compression are realized in the bi-directional scheme. The fiber length in the architecture is numerically optimized, and the proposed quantization scheme is evaluated by both simulation and experiment in the case of N = 2. In the experiment, a quantization resolution of 6.2 bits is obtained, which is 1.2-bit higher than that of its uni-directional counterpart.

Non-Invasive Measurement of Frog Skin Reflectivity in High Spatial Resolution Using a Dual Hyperspectral Approach

PubMed Central

Liebisch, Frank; Walter, Achim; Greven, Hartmut; Rascher, Uwe

2013-01-01

Background Most spectral data for the amphibian integument are limited to the visible spectrum of light and have been collected using point measurements with low spatial resolution. In the present study a dual camera setup consisting of two push broom hyperspectral imaging systems was employed, which produces reflectance images between 400 and 2500 nm with high spectral and spatial resolution and a high dynamic range. Methodology/Principal Findings We briefly introduce the system and document the high efficiency of this technique analyzing exemplarily the spectral reflectivity of the integument of three arboreal anuran species (Litoria caerulea, Agalychnis callidryas and Hyla arborea), all of which appear green to the human eye. The imaging setup generates a high number of spectral bands within seconds and allows non-invasive characterization of spectral characteristics with relatively high working distance. Despite the comparatively uniform coloration, spectral reflectivity between 700 and 1100 nm differed markedly among the species. In contrast to H. arborea, L. caerulea and A. callidryas showed reflection in this range. For all three species, reflectivity above 1100 nm is primarily defined by water absorption. Furthermore, the high resolution allowed examining even small structures such as fingers and toes, which in A. callidryas showed an increased reflectivity in the near infrared part of the spectrum. Conclusion/Significance Hyperspectral imaging was found to be a very useful alternative technique combining the spectral resolution of spectrometric measurements with a higher spatial resolution. In addition, we used Digital Infrared/Red-Edge Photography as new simple method to roughly determine the near infrared reflectivity of frog specimens in field, where hyperspectral imaging is typically difficult. PMID:24058464

A novel capacitive absolute positioning sensor based on time grating with nanometer resolution

NASA Astrophysics Data System (ADS)

Pu, Hongji; Liu, Hongzhong; Liu, Xiaokang; Peng, Kai; Yu, Zhicheng

2018-05-01

The present work proposes a novel capacitive absolute positioning sensor based on time grating. The sensor includes a fine incremental-displacement measurement component combined with a coarse absolute-position measurement component to obtain high-resolution absolute positioning measurements. A single row type sensor was proposed to achieve fine displacement measurement, which combines the two electrode rows of a previously proposed double-row type capacitive displacement sensor based on time grating into a single row. To achieve absolute positioning measurement, the coarse measurement component is designed as a single-row type displacement sensor employing a single spatial period over the entire measurement range. In addition, this component employs a rectangular induction electrode and four groups of orthogonal discrete excitation electrodes with half-sinusoidal envelope shapes, which were formed by alternately extending the rectangular electrodes of the fine measurement component. The fine and coarse measurement components are tightly integrated to form a compact absolute positioning sensor. A prototype sensor was manufactured using printed circuit board technology for testing and optimization of the design in conjunction with simulations. Experimental results show that the prototype sensor achieves a ±300 nm measurement accuracy with a 1 nm resolution over a displacement range of 200 mm when employing error compensation. The proposed sensor is an excellent alternative to presently available long-range absolute nanometrology sensors owing to its low cost, simple structure, and ease of manufacturing.

Droplet Image Super Resolution Based on Sparse Representation and Kernel Regression

NASA Astrophysics Data System (ADS)

Zou, Zhenzhen; Luo, Xinghong; Yu, Qiang

2018-02-01

Microgravity and containerless conditions, which are produced via electrostatic levitation combined with a drop tube, are important when studying the intrinsic properties of new metastable materials. Generally, temperature and image sensors can be used to measure the changes of sample temperature, morphology and volume. Then, the specific heat, surface tension, viscosity changes and sample density can be obtained. Considering that the falling speed of the material sample droplet is approximately 31.3 m/s when it reaches the bottom of a 50-meter-high drop tube, a high-speed camera with a collection rate of up to 106 frames/s is required to image the falling droplet. However, at the high-speed mode, very few pixels, approximately 48-120, will be obtained in each exposure time, which results in low image quality. Super-resolution image reconstruction is an algorithm that provides finer details than the sampling grid of a given imaging device by increasing the number of pixels per unit area in the image. In this work, we demonstrate the application of single image-resolution reconstruction in the microgravity and electrostatic levitation for the first time. Here, using the image super-resolution method based on sparse representation, a low-resolution droplet image can be reconstructed. Employed Yang's related dictionary model, high- and low-resolution image patches were combined with dictionary training, and high- and low-resolution-related dictionaries were obtained. The online double-sparse dictionary training algorithm was used in the study of related dictionaries and overcome the shortcomings of the traditional training algorithm with small image patch. During the stage of image reconstruction, the algorithm of kernel regression is added, which effectively overcomes the shortcomings of the Yang image's edge blurs.

Droplet Image Super Resolution Based on Sparse Representation and Kernel Regression

NASA Astrophysics Data System (ADS)

Zou, Zhenzhen; Luo, Xinghong; Yu, Qiang

2018-05-01

Microgravity and containerless conditions, which are produced via electrostatic levitation combined with a drop tube, are important when studying the intrinsic properties of new metastable materials. Generally, temperature and image sensors can be used to measure the changes of sample temperature, morphology and volume. Then, the specific heat, surface tension, viscosity changes and sample density can be obtained. Considering that the falling speed of the material sample droplet is approximately 31.3 m/s when it reaches the bottom of a 50-meter-high drop tube, a high-speed camera with a collection rate of up to 106 frames/s is required to image the falling droplet. However, at the high-speed mode, very few pixels, approximately 48-120, will be obtained in each exposure time, which results in low image quality. Super-resolution image reconstruction is an algorithm that provides finer details than the sampling grid of a given imaging device by increasing the number of pixels per unit area in the image. In this work, we demonstrate the application of single image-resolution reconstruction in the microgravity and electrostatic levitation for the first time. Here, using the image super-resolution method based on sparse representation, a low-resolution droplet image can be reconstructed. Employed Yang's related dictionary model, high- and low-resolution image patches were combined with dictionary training, and high- and low-resolution-related dictionaries were obtained. The online double-sparse dictionary training algorithm was used in the study of related dictionaries and overcome the shortcomings of the traditional training algorithm with small image patch. During the stage of image reconstruction, the algorithm of kernel regression is added, which effectively overcomes the shortcomings of the Yang image's edge blurs.

Optimized multiple linear mappings for single image super-resolution

NASA Astrophysics Data System (ADS)

Zhang, Kaibing; Li, Jie; Xiong, Zenggang; Liu, Xiuping; Gao, Xinbo

2017-12-01

Learning piecewise linear regression has been recognized as an effective way for example learning-based single image super-resolution (SR) in literature. In this paper, we employ an expectation-maximization (EM) algorithm to further improve the SR performance of our previous multiple linear mappings (MLM) based SR method. In the training stage, the proposed method starts with a set of linear regressors obtained by the MLM-based method, and then jointly optimizes the clustering results and the low- and high-resolution subdictionary pairs for regression functions by using the metric of the reconstruction errors. In the test stage, we select the optimal regressor for SR reconstruction by accumulating the reconstruction errors of m-nearest neighbors in the training set. Thorough experimental results carried on six publicly available datasets demonstrate that the proposed SR method can yield high-quality images with finer details and sharper edges in terms of both quantitative and perceptual image quality assessments.

Atomic resolution ADF-STEM imaging of organic molecular crystal of halogenated copper phthalocyanine.

PubMed

Haruta, Mitsutaka; Yoshida, Kaname; Kurata, Hiroki; Isoda, Seiji

2008-05-01

Annular dark-field (ADF) scanning transmission electron microscopy (STEM) measurements are demonstrated for the first time to be applicable for acquiring Z-contrast images of organic molecules at atomic resolution. High-angle ADF imaging by STEM is a new technique that provides incoherent high-resolution Z-contrast images for organic molecules. In the present study, low-angle ADF-STEM is successfully employed to image the molecular crystal structure of hexadecachloro-Cu-phthalocyanine (Cl16-CuPc), an organic molecule. The structures of CuPc derivatives (polyhalogenated CuPc with Br and Cl) are determined quantitatively using the same technique to determine the occupancy of halogens at each chemical site. By comparing the image contrasts of atomic columns, the occupancy of Br is found to be ca. 56% at the inner position, slightly higher than that for random substitution and in good agreement with previous TEM results.

Short range, ultra-wideband radar with high resolution swept range gate

DOEpatents

McEwan, T.E.

1998-05-26

A radar range finder and hidden object locator is based on ultra-wide band radar with a high resolution swept range gate. The device generates an equivalent time amplitude scan with a typical range of 4 inches to 20 feet, and an analog range resolution as limited by a jitter of on the order of 0.01 inches. A differential sampling receiver is employed to effectively eliminate ringing and other aberrations induced in the receiver by the near proximity of the transmit antenna, so a background subtraction is not needed, simplifying the circuitry while improving performance. Uses of the invention include a replacement of ultrasound devices for fluid level sensing, automotive radar, such as cruise control and parking assistance, hidden object location, such as stud and rebar finding. Also, this technology can be used when positioned over a highway lane to collect vehicle count and speed data for traffic control. 14 figs.

Short range, ultra-wideband radar with high resolution swept range gate

DOEpatents

McEwan, Thomas E.

1998-05-26

A radar range finder and hidden object locator is based on ultra-wide band radar with a high resolution swept range gate. The device generates an equivalent time amplitude scan with a typical range of 4 inches to 20 feet, and an analog range resolution as limited by a jitter of on the order of 0.01 inches. A differential sampling receiver is employed to effectively eliminate ringing and other aberrations induced in the receiver by the near proximity of the transmit antenna, so a background subtraction is not needed, simplifying the circuitry while improving performance. Uses of the invention include a replacement of ultrasound devices for fluid level sensing, automotive radar, such as cruise control and parking assistance, hidden object location, such as stud and rebar finding. Also, this technology can be used when positioned over a highway lane to collect vehicle count and speed data for traffic control.

A metrology system for a high resolution cavity beam position monitor system

NASA Astrophysics Data System (ADS)

Walston, Sean; Boogert, Stewart; Chung, Carl; Fitsos, Pete; Frisch, Joe; Gronberg, Jeff; Hayano, Hitoshi; Hinton, Shantell; Honda, Yosuke; Khainovski, Oleg; Kolomensky, Yury; Loscutoff, Peter; Lyapin, Alexey; Malton, Stephen; May, Justin; McCormick, Douglas; Meller, Robert; Miller, David; Orimoto, Toyoko; Ross, Marc; Slater, Mark; Smith, Steve; Smith, Tonee; Terunuma, Nobuhiro; Thomson, Mark; Urakawa, Junji; Vogel, Vladimir; Ward, David; White, Glen

2013-11-01

International Linear Collider (ILC) interaction region beam sizes and component position stability requirements will likely be as small as a few nanometers. It is important to the ILC design effort to demonstrate that these tolerances can be achieved-ideally using a beam-based stability measurement. We developed a high resolution RF cavity Beam Position Monitor (BPM) system. A triplet of these BPMs, installed in the extraction line of the KEK Accelerator Test Facility (ATF) and tested with its ultra-low emittance beam, achieved a position measurement resolution of 15 nm. A metrology system for the three BPMs was subsequently installed. This system employed optical encoders to measure each BPM's position and orientation relative to a zero-coefficient of thermal expansion carbon fiber frame. We have demonstrated that the three BPMs behave as a rigid-body at the level of less than 5 nm.

Novel strategy for the determination of illegal adulterants in health foods and herbal medicines using high-performance liquid chromatography with high-resolution mass spectrometry.

PubMed

Wang, Zhe; Wu, Caisheng; Wang, Gangli; Zhang, Qingsheng; Zhang, Jinlan

2015-03-01

The detection, confirmation, and quantification of multiple illegal adulterants in health foods and herbal medicines by using a single analytical method are a challenge. This paper reports on a new strategy to meet this challenge by employing high-performance liquid chromatography coupled with high-resolution mass spectrometry and a mass spectral tree similarity filter technique. This analytical method can rapidly collect high-resolution, high-accuracy, optionally multistage mass data for compounds in samples. After a preliminary screening by retention time and high-resolution mass spectral data, known illegal adulterants can be detected. The mass spectral tree similarity filter technique has been applied to rapidly confirm these adulterants and simultaneously discover unknown ones. By using full-scan mass spectra as stem and data-dependent subsequent stage mass spectra to form branches, mass spectrometry data from detected compounds are converted into mass spectral trees. The known or unknown illegal adulterants in the samples are confirmed or discovered based on the similarity between their mass spectral trees and those of the references in a library, and they are finally quantified against standard curves. This new strategy has been tested by using 50 samples, and the illegal adulterants were rapidly and effectively detected, confirmed and quantified. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Comparing High Definition Live Interactive and Store-and-Forward Consultations to In-Person Examinations

PubMed Central

Locatis, Craig; Burges, Gene; Maisiak, Richard; Liu, Wei-Li; Ackerman, Michael

2017-01-01

Abstract Background: There is little teledermatology research directly comparing remote methods, even less research with two in-person dermatologist agreement providing a baseline for comparing remote methods, and no research using high definition video as a live interactive method. Objective: To compare in-person consultations with store-and-forward and live interactive methods, the latter having two levels of image quality. Methods: A controlled study was conducted where patients were examined in-person, by high definition video, and by store-and-forward methods. The order patients experienced methods and residents assigned methods rotated, although an attending always saw patients in-person. The type of high definition video employed, lower resolution compressed or higher resolution uncompressed, was alternated between clinics. Primary and differential diagnoses, biopsy recommendations, and diagnostic and biopsy confidence ratings were recorded. Results: Concordance and confidence were significantly better for in-person versus remote methods and biopsy recommendations were lower. Store-and-forward and higher resolution uncompressed video results were similar and better than those for lower resolution compressed video. Limitations: Dermatology residents took store-and-forward photos and their quality was likely superior to those normally taken in practice. There were variations in expertise between the attending and second and third year residents. Conclusion: The superiority of in-person consultations suggests the tendencies to order more biopsies or still see patients in-person are often justified in teledermatology and that high resolution uncompressed video can close the resolution gap between store-and-forward and live interactive methods. PMID:27705083

High-Resolution Topography and its Implications for the Formation of Europa's Ridged Plains

NASA Astrophysics Data System (ADS)

Leonard, E. J.; Pappalardo, R. T.; Yin, A.; Patthoff, D. A.; Schenk, P.

2015-12-01

The Galileo Solid State Imager (SSI) recorded nine very high-resolution frames—eight at 12 m/pixel and one at 6 m/pixel—during the E12 flyby of Europa in Dec. 1997. To understand the implications for the small-scale structure and evolution of Europa, we mosaicked these frames (observations 12ESMOTTLE01 and 02, incidence ≈18°, emission ≈77°) into their regional context (part of observation 11ESREGMAP01, 220 m/pixel, incidence ≈74°, emission ≈23°). The topography data, which was created from the image mosaic overlaps, is sparse and segmented over the high-resolution images but connected by the underlying regional resolution topography. The high-resolution topography (24 m/pixel) is among the best for the current Europan dataset. From this dataset we ascertain the root mean square, or RMS, slope for some of the most common Europan surface features in a new region. We also employ a Fourier Transform method previously used on Ganymede and on other areas of Europa (Patel et al., 1999 JGR), to derive common wavelengths for the subunits of the ubiquitous ridged plains terrain. These results have important implications for differentiating between possible formation mechanisms—extensional tilt blocks (Pappalardo et al., 1995 JGR) or folds (Leonard et al., 2015 LPSC Abstract)—and for potential future missions. We continue this method for another high-resolution region taken in the E12 orbit, WEDGES01 and 02, with the specific goal of investigating how the variations in ridged plains morphologies relate across the surface of Europa.

A New High Resolution Climate Dataset for Climate Change Impacts Assessments in New England

NASA Astrophysics Data System (ADS)

Komurcu, M.; Huber, M.

2016-12-01

Assessing regional impacts of climate change (such as changes in extreme events, land surface hydrology, water resources, energy, ecosystems and economy) requires much higher resolution climate variables than those available from global model projections. While it is possible to run global models in higher resolution, the high computational cost associated with these simulations prevent their use in such manner. To alleviate this problem, dynamical downscaling offers a method to deliver higher resolution climate variables. As part of an NSF EPSCoR funded interdisciplinary effort to assess climate change impacts on New Hampshire ecosystems, hydrology and economy (the New Hampshire Ecosystems and Society project), we create a unique high-resolution climate dataset for New England. We dynamically downscale global model projections under a high impact emissions scenario using the Weather Research and Forecasting model (WRF) with three nested grids of 27, 9 and 3 km horizontal resolution with the highest resolution innermost grid focusing over New England. We prefer dynamical downscaling over other methods such as statistical downscaling because it employs physical equations to progressively simulate climate variables as atmospheric processes interact with surface processes, emissions, radiation, clouds, precipitation and other model components, hence eliminates fix relationships between variables. In addition to simulating mean changes in regional climate, dynamical downscaling also allows for the simulation of climate extremes that significantly alter climate change impacts. We simulate three time slices: 2006-2015, 2040-2060 and 2080-2100. This new high-resolution climate dataset (with more than 200 variables saved in hourly (six hourly) intervals for the highest resolution domain (outer two domains)) along with model input and restart files used in our WRF simulations will be publicly available for use to the broader scientific community to support in-depth climate change impacts assessments for New England. We present results focusing on future changes in New England extreme events.

Optical system for high resolution spectrometer/monochromator

DOEpatents

Hettrick, Michael C.; Underwood, James H.

1988-01-01

An optical system for use in a spectrometer or monochromator employing a mirror which reflects electromagnetic radiation from a source to converge with same in a plane. A straight grooved, varied-spaced diffraction grating receives the converging electromagnetic radiation from the mirror and produces a spectral image for capture by a detector, target or like receiver.

Near-electrode imager

DOEpatents

Rathke, Jerome W.; Klingler, Robert J.; Woelk, Klaus; Gerald, II, Rex E.

2000-01-01

An apparatus, near-electrode imager, for employing nuclear magnetic resonance imaging to provide in situ measurements of electrochemical properties of a sample as a function of distance from a working electrode. The near-electrode imager uses the radio frequency field gradient within a cylindrical toroid cavity resonator to provide high-resolution nuclear magnetic resonance spectral information on electrolyte materials.

Cr{sub 2}O{sub 5} as new cathode for rechargeable sodium ion batteries

DOE Office of Scientific and Technical Information (OSTI.GOV)

Feng, Xu-Yong; Chien, Po-Hsiu; Rose, Alyssa M.

2016-10-15

Chromium oxide, Cr{sub 2}O{sub 5}, was synthesized by pyrolyzing CrO{sub 3} at 350 °C and employed as a new cathode in rechargeable sodium ion batteries. Cr{sub 2}O{sub 5}/Na rechargeable batteries delivered high specific capacities up to 310 mAh/g at a current density of C/16 (or 20 mA/g). High-resolution solid-state {sup 23}Na NMR both qualitatively and quantitatively revealed the reversible intercalation of Na ions into the bulk electrode and participation of Na ions in the formation of the solid-electrolyte interphase largely at low potentials. Amorphization of the electrode structure occurred during the first discharge revealed by both NMR and X-ray diffractionmore » data. CrO{sub 3}-catalyzed electrolyte degradation and loss in electronic conductivity led to gradual capacity fading. The specific capacity stabilized at >120 mAh/g after 50 charge-discharge cycles. Further improvement in electrochemical performance is possible via electrode surface modification, polymer binder incorporation, or designs of new morphologies. - Graphical abstract: Electrochemical profile of a Cr{sub 2}O{sub 5}/Na battery cell and high-resolution solid-state {sup 23}Na MAS NMR spectrum of a Cr{sub 2}O{sub 5} electrode discharged to 2 V. - Highlights: • Cr{sub 2}O{sub 5} was synthesized and used as a new cathode in rechargeable Na ion batteries. • A high capacity of 310 mAh/g and an energy density of 564 Wh/kg were achieved. • High-resolution solid-state {sup 23}Na NMR was employed to follow the reaction mechanisms.« less

Effect of Te inclusions in CdZnTe crystals at different temperatures

NASA Astrophysics Data System (ADS)

Hossain, A.; Bolotnikov, A. E.; Camarda, G. S.; Gul, R.; Kim, K.-H.; Cui, Y.; Yang, G.; Xu, L.; James, R. B.

2011-02-01

CdZnTe crystals often exhibit nonuniformities due to the presence of Te inclusions and dislocations. High concentrations of such defects in these crystals generally entail severe charge-trapping, a major problem in ensuring the device's satisfactory performance. In this study, we employed a high-intensity, high-spatial-resolution synchrotron x-ray beam as the ideal tool to generate charges by focusing it over the large Te inclusions, and then observing the carrier's response at room- and at low-temperatures. A high spatial 5-μm resolution raster scan revealed the fine details of the presence of extended defects, like Te inclusions and dislocations in the CdZnTe crystals. A noticeable change was observed in the efficiency of electron charge collection at low temperature (1 °C), but it was hardly altered at room-temperature.

An integrated signal conditioner for high-frequency inductive position sensors

NASA Astrophysics Data System (ADS)

Rahal, Mohamad; Demosthenous, Andreas

2010-01-01

This paper describes the design, implementation and evaluation of a signal conditioner application-specific integrated circuit (ASIC) for high-frequency inductive non-contact position sensors. These sensors employ a radio frequency technology based on an antenna planar arrangement and a resonant target, have a high inherent resolution (0.1% of antenna length) and can measure target position over a wide distance range (<0.1 mm to >10 m). However, due to the relatively high-frequency excitation (1 MHz typically) and to the specific layouts of these sensors, there is unwanted capacitive coupling between the transmitter and receiver coils; this type of distortion reduces linearity and resolution. The ASIC, which is the first generation of its kind for this type of sensor, employs a differential mixer topology which suppresses the capacitive coupling offsets. The system architecture and circuit details are presented. The ASIC was fabricated in a 0.6 µm high-voltage CMOS technology occupying an area of 8 mm2. It dissipates about 30 mA from a 24 V power supply. The ASIC was tested with a high-frequency inductive position sensor (with an antenna length of 10.8 cm). The measured input-referred offset due to transmitter crosstalk is on average about 22 µV over a wide phase difference variation (-99° to +117°) between the transmitter and demodulating signals.

WINERED: a warm near-infrared high-resolution spectrograph

NASA Astrophysics Data System (ADS)

Ikeda, Yuji; Kobayashi, Naoto; Kondo, Sohei; Yasui, Chikako; Motohara, Kentaro; Minami, Atsushi

2006-06-01

We are developing a new near-infrared high-resolution (R max = 100,000) and high-sensitive spectrograph WINERED, which is specifically customized for short NIR bands at 0.9-1.35 μm. WINERED employs the following two novel approaches in the optical system: (1) portable design with a ZnSe immersion grating and (2) warm optics without any cold stops. These concepts result in several essential advantages as follows: easy to build, align, and maintain; these result in a short development time and low cost. WINERED employs a VIRGO HgCdTe 2k × 2k array by Raytheon as the detector. We are developing our own array control system that aims at a low readout noise (< 10 e -) with a readout time of about 3 sec. Our goal is to achieve a high sensitivity of R = 100,000 for a NIR spectroscopy of 15 mag and 17 mag point sources with 4 m and 10 m telescopes, respectively. We have just finalized the optical design and produced a prototype electronics, which are described in the companion papers by Yasui et al. and Kondo et al., respectively. We plan to complete this instrument by the end of 2008 and hope to attach it to various 4 to 10 m telescopes as a PI-type instrument.

Revealing fine microstructural morphology in the living human retina using Optical Coherence Tomography with pancorrection

NASA Astrophysics Data System (ADS)

Torti, C.; Považay, B.; Hofer, B.; Unterhuber, A.; Hermann, B.; Drexler, W.

2008-09-01

Ultra-high speed optical coherence tomography employing an ultra-broadband light source has been combined with adaptive optics utilizing a single high stroke deformable mirror and chromatic aberration compensation. The reduction of motion artefacts, geometric and chromatic aberrations (pancorrection) permits to achieve an isotropic resolution of 2-3 μm in the human eye. The performance of this non-invasive imaging modality enables to resolve cellular structures including cone photoreceptors, nerve fibre bundles and collagenous plates of the lamina cribrosa, and retinal pigment epithelial (RPE) cells in the human retina in vivo with superior detail. Alterations of cellular morphology due to cone degeneration in a colour-blind subject are investigated in ultra-high resolution with selective depth sectioning for the first time.

Fluid Lensing based Machine Learning for Augmenting Earth Science Coral Datasets

NASA Astrophysics Data System (ADS)

Li, A.; Instrella, R.; Chirayath, V.

2016-12-01

Recently, there has been increased interest in monitoring the effects of climate change upon the world's marine ecosystems, particularly coral reefs. These delicate ecosystems are especially threatened due to their sensitivity to ocean warming and acidification, leading to unprecedented levels of coral bleaching and die-off in recent years. However, current global aquatic remote sensing datasets are unable to quantify changes in marine ecosystems at spatial and temporal scales relevant to their growth. In this project, we employ various supervised and unsupervised machine learning algorithms to augment existing datasets from NASA's Earth Observing System (EOS), using high resolution airborne imagery. This method utilizes NASA's ongoing airborne campaigns as well as its spaceborne assets to collect remote sensing data over these afflicted regions, and employs Fluid Lensing algorithms to resolve optical distortions caused by the fluid surface, producing cm-scale resolution imagery of these diverse ecosystems from airborne platforms. Support Vector Machines (SVMs) and K-mean clustering methods were applied to satellite imagery at 0.5m resolution, producing segmented maps classifying coral based on percent cover and morphology. Compared to a previous study using multidimensional maximum a posteriori (MAP) estimation to separate these features in high resolution airborne datasets, SVMs are able to achieve above 75% accuracy when augmented with existing MAP estimates, while unsupervised methods such as K-means achieve roughly 68% accuracy, verified by manually segmented reference data provided by a marine biologist. This effort thus has broad applications for coastal remote sensing, by helping marine biologists quantify behavioral trends spanning large areas and over longer timescales, and to assess the health of coral reefs worldwide.

Los Angeles megacity: a high-resolution land–atmosphere modelling system for urban CO 2 emissions

DOE PAGES

Feng, Sha; Lauvaux, Thomas; Newman, Sally; ...

2016-07-22

Megacities are major sources of anthropogenic fossil fuel CO 2 (FFCO 2) emissions. The spatial extents of these large urban systems cover areas of 10 000 km 2 or more with complex topography and changing landscapes. We present a high-resolution land–atmosphere modelling system for urban CO 2 emissions over the Los Angeles (LA) megacity area. The Weather Research and Forecasting (WRF)-Chem model was coupled to a very high-resolution FFCO 2 emission product, Hestia-LA, to simulate atmospheric CO 2 concentrations across the LA megacity at spatial resolutions as fine as ~1 km. We evaluated multiple WRF configurations, selecting one that minimizedmore » errors in wind speed, wind direction, and boundary layer height as evaluated by its performance against meteorological data collected during the CalNex-LA campaign (May–June 2010). Our results show no significant difference between moderate-resolution (4 km) and high-resolution (1.3 km) simulations when evaluated against surface meteorological data, but the high-resolution configurations better resolved planetary boundary layer heights and vertical gradients in the horizontal mean winds. We coupled our WRF configuration with the Vulcan 2.2 (10 km resolution) and Hestia-LA (1.3 km resolution) fossil fuel CO 2 emission products to evaluate the impact of the spatial resolution of the CO 2 emission products and the meteorological transport model on the representation of spatiotemporal variability in simulated atmospheric CO 2 concentrations. We find that high spatial resolution in the fossil fuel CO 2 emissions is more important than in the atmospheric model to capture CO 2 concentration variability across the LA megacity. Finally, we present a novel approach that employs simultaneous correlations of the simulated atmospheric CO 2 fields to qualitatively evaluate the greenhouse gas measurement network over the LA megacity. Spatial correlations in the atmospheric CO 2 fields reflect the coverage of individual measurement sites when a statistically significant number of sites observe emissions from a specific source or location. We conclude that elevated atmospheric CO 2 concentrations over the LA megacity are composed of multiple fine-scale plumes rather than a single homogenous urban dome. Furthermore, we conclude that FFCO 2 emissions monitoring in the LA megacity requires FFCO 2 emissions modelling with ~1 km resolution because coarser-resolution emissions modelling tends to overestimate the observational constraints on the emissions estimates.« less

Los Angeles megacity: a high-resolution land–atmosphere modelling system for urban CO 2 emissions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Feng, Sha; Lauvaux, Thomas; Newman, Sally

Megacities are major sources of anthropogenic fossil fuel CO 2 (FFCO 2) emissions. The spatial extents of these large urban systems cover areas of 10 000 km 2 or more with complex topography and changing landscapes. We present a high-resolution land–atmosphere modelling system for urban CO 2 emissions over the Los Angeles (LA) megacity area. The Weather Research and Forecasting (WRF)-Chem model was coupled to a very high-resolution FFCO 2 emission product, Hestia-LA, to simulate atmospheric CO 2 concentrations across the LA megacity at spatial resolutions as fine as ~1 km. We evaluated multiple WRF configurations, selecting one that minimizedmore » errors in wind speed, wind direction, and boundary layer height as evaluated by its performance against meteorological data collected during the CalNex-LA campaign (May–June 2010). Our results show no significant difference between moderate-resolution (4 km) and high-resolution (1.3 km) simulations when evaluated against surface meteorological data, but the high-resolution configurations better resolved planetary boundary layer heights and vertical gradients in the horizontal mean winds. We coupled our WRF configuration with the Vulcan 2.2 (10 km resolution) and Hestia-LA (1.3 km resolution) fossil fuel CO 2 emission products to evaluate the impact of the spatial resolution of the CO 2 emission products and the meteorological transport model on the representation of spatiotemporal variability in simulated atmospheric CO 2 concentrations. We find that high spatial resolution in the fossil fuel CO 2 emissions is more important than in the atmospheric model to capture CO 2 concentration variability across the LA megacity. Finally, we present a novel approach that employs simultaneous correlations of the simulated atmospheric CO 2 fields to qualitatively evaluate the greenhouse gas measurement network over the LA megacity. Spatial correlations in the atmospheric CO 2 fields reflect the coverage of individual measurement sites when a statistically significant number of sites observe emissions from a specific source or location. We conclude that elevated atmospheric CO 2 concentrations over the LA megacity are composed of multiple fine-scale plumes rather than a single homogenous urban dome. Furthermore, we conclude that FFCO 2 emissions monitoring in the LA megacity requires FFCO 2 emissions modelling with ~1 km resolution because coarser-resolution emissions modelling tends to overestimate the observational constraints on the emissions estimates.« less

Digital micromirror device-based laser-illumination Fourier ptychographic microscopy

PubMed Central

Kuang, Cuifang; Ma, Ye; Zhou, Renjie; Lee, Justin; Barbastathis, George; Dasari, Ramachandra R.; Yaqoob, Zahid; So, Peter T. C.

2015-01-01

We report a novel approach to Fourier ptychographic microscopy (FPM) by using a digital micromirror device (DMD) and a coherent laser source (532 nm) for generating spatially modulated sample illumination. Previously demonstrated FPM systems are all based on partially-coherent illumination, which offers limited throughput due to insufficient brightness. Our FPM employs a high power coherent laser source to enable shot-noise limited high-speed imaging. For the first time, a digital micromirror device (DMD), imaged onto the back focal plane of the illumination objective, is used to generate spatially modulated sample illumination field for ptychography. By coding the on/off states of the micromirrors, the illumination plane wave angle can be varied at speeds more than 4 kHz. A set of intensity images, resulting from different oblique illuminations, are used to numerically reconstruct one high-resolution image without obvious laser speckle. Experiments were conducted using a USAF resolution target and a fiber sample, demonstrating high-resolution imaging capability of our system. We envision that our approach, if combined with a coded-aperture compressive-sensing algorithm, will further improve the imaging speed in DMD-based FPM systems. PMID:26480361

Digital micromirror device-based laser-illumination Fourier ptychographic microscopy.

PubMed

Kuang, Cuifang; Ma, Ye; Zhou, Renjie; Lee, Justin; Barbastathis, George; Dasari, Ramachandra R; Yaqoob, Zahid; So, Peter T C

2015-10-19

We report a novel approach to Fourier ptychographic microscopy (FPM) by using a digital micromirror device (DMD) and a coherent laser source (532 nm) for generating spatially modulated sample illumination. Previously demonstrated FPM systems are all based on partially-coherent illumination, which offers limited throughput due to insufficient brightness. Our FPM employs a high power coherent laser source to enable shot-noise limited high-speed imaging. For the first time, a digital micromirror device (DMD), imaged onto the back focal plane of the illumination objective, is used to generate spatially modulated sample illumination field for ptychography. By coding the on/off states of the micromirrors, the illumination plane wave angle can be varied at speeds more than 4 kHz. A set of intensity images, resulting from different oblique illuminations, are used to numerically reconstruct one high-resolution image without obvious laser speckle. Experiments were conducted using a USAF resolution target and a fiber sample, demonstrating high-resolution imaging capability of our system. We envision that our approach, if combined with a coded-aperture compressive-sensing algorithm, will further improve the imaging speed in DMD-based FPM systems.

Conflict Resolution in Marriage: Toward a Theory of Spousal Strategies and Marital Dissolution Rates.

ERIC Educational Resources Information Center

Chafetz, Janet Saltzman

1980-01-01

There are four strategies spouses may attempt to employ in cases of conflict: authority, control, influence, and manipulation. Rates of marital dissolution are a function of the relative equality between spouses in terms of the types of conflict-resolution strategies they are able to employ. (Author)

Application of high-resolution, two-dimensional 1H and 13C nuclear magnetic resonance techniques to the characterization of lipid oxidation products in autoxidized linoleoyl/linolenoylglycerols.

PubMed

Silwood, C J; Grootveld, M

1999-07-01

Subjection of polyunsaturated fatty acid (PUFA)-rich culinary oils to standard frying episodes generates a range of lipid oxidation products (LOP), including saturated and alpha,beta-unsaturated aldehydes which arise from the thermally induced fragmentation of conjugated hydroperoxydiene precursors. Since such LOP are damaging to human health, we have employed high-resolution, two-dimensional 1H-1H relayed coherence transfer, 1H-1H total correlation, 1H-13C heteronuclear multiple quantum correlation, and 1H-1H J-resolved nuclear magnetic resonance (NMR) spectroscopic techniques to further elucidate the molecular structures of these components present in (i) a model linoleoylglycerol compound (1,3-dilinolein) allowed to autoxidize at ambient temperature and (ii) PUFA-rich culinary oils subjected to repeated frying episodes. The above techniques readily facilitate the resolution of selected vinylic and aldehydic resonances of LOP which appear as complex overlapping patterns in conventional one-dimensional spectra, particularly when employed in combination with solvent-induced spectral shift modifications. Hence, much useful multi-component information regarding the identity and/or classification of glycerol-bound conjugated hydroperoxydiene and hydroxydiene adducts, and saturated and alpha,beta-unsaturated aldehydes, present in autoxidized PUFA matrices is provided by these NMR methods. Such molecular information is of much value to researchers investigating the deleterious health effects of LOP available in the diet.

High-resolution measurements of the spatial and temporal evolution of megagauss magnetic fields created in intense short-pulse laser-plasma interactions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chatterjee, Gourab, E-mail: gourab@tifr.res.in; Singh, Prashant Kumar; Adak, Amitava

A pump-probe polarimetric technique is demonstrated, which provides a complete, temporally and spatially resolved mapping of the megagauss magnetic fields generated in intense short-pulse laser-plasma interactions. A normally incident time-delayed probe pulse reflected from its critical surface undergoes a change in its ellipticity according to the magneto-optic Cotton-Mouton effect due to the azimuthal nature of the ambient self-generated megagauss magnetic fields. The temporal resolution of the magnetic field mapping is typically of the order of the pulsewidth, limited by the laser intensity contrast, whereas a spatial resolution of a few μm is achieved by this optical technique. High-harmonics of themore » probe can be employed to penetrate deeper into the plasma to even near-solid densities. The spatial and temporal evolution of the megagauss magnetic fields at the target front as well as at the target rear are presented. The μm-scale resolution of the magnetic field mapping provides valuable information on the filamentary instabilities at the target front, whereas probing the target rear mirrors the highly complex fast electron transport in intense laser-plasma interactions.« less

Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field

NASA Astrophysics Data System (ADS)

Curatolo, Andrea; Villiger, Martin; Lorenser, Dirk; Wijesinghe, Philip; Fritz, Alexander; Kennedy, Brendan F.; Sampson, David D.

2016-03-01

Visualizing stiffness within the local tissue environment at the cellular and sub-cellular level promises to provide insight into the genesis and progression of disease. In this paper, we propose ultrahigh-resolution optical coherence elastography, and demonstrate three-dimensional imaging of local axial strain of tissues undergoing compressive loading. The technique employs a dual-arm extended focus optical coherence microscope to measure tissue displacement under compression. The system uses a broad bandwidth supercontinuum source for ultrahigh axial resolution, Bessel beam illumination and Gaussian beam detection, maintaining sub-2 μm transverse resolution over nearly 100 μm depth of field, and spectral-domain detection allowing high displacement sensitivity. The system produces strain elastograms with a record resolution (x,y,z) of 2×2×15 μm. We benchmark the advances in terms of resolution and strain sensitivity by imaging a suitable inclusion phantom. We also demonstrate this performance on freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography system.

Calculation of the spatial resolution in two-photon absorption spectroscopy applied to plasma diagnosis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Garcia-Lechuga, M.; Laser Processing Group, Instituto de Óptica “Daza de Valdés,” CSIC, 28006-Madrid; Fuentes, L. M.

2014-10-07

We report a detailed characterization of the spatial resolution provided by two-photon absorption spectroscopy suited for plasma diagnosis via the 1S-2S transition of atomic hydrogen for optogalvanic detection and laser induced fluorescence (LIF). A precise knowledge of the spatial resolution is crucial for a correct interpretation of measurements, if the plasma parameters to be analysed undergo strong spatial variations. The present study is based on a novel approach which provides a reliable and realistic determination of the spatial resolution. Measured irradiance distribution of laser beam waists in the overlap volume, provided by a high resolution UV camera, are employed tomore » resolve coupled rate equations accounting for two-photon excitation, fluorescence decay and ionization. The resulting three-dimensional yield distributions reveal in detail the spatial resolution for optogalvanic and LIF detection and related saturation due to depletion. Two-photon absorption profiles broader than the Fourier transform-limited laser bandwidth are also incorporated in the calculations. The approach allows an accurate analysis of the spatial resolution present in recent and future measurements.« less

Application of high-speed photography to chip refining

NASA Astrophysics Data System (ADS)

Stationwala, Mustafa I.; Miller, Charles E.; Atack, Douglas; Karnis, A.

1991-04-01

Several high speed photographic methods have been employed to elucidate the mechanistic aspects of producing mechanical pulp in a disc refiner. Material flow patterns of pulp in a refmer were previously recorded by means of a HYCAM camera and continuous lighting system which provided cine pictures at up to 10,000 pps. In the present work an IMACON camera was used to obtain several series of high resolution, high speed photographs, each photograph containing an eight-frame sequence obtained at a framing rate of 100,000 pps. These high-resolution photographs made it possible to identify the nature of the fibrous material trapped on the bars of the stationary disc. Tangential movement of fibre floes, during the passage of bars on the rotating disc over bars on the stationary disc, was also observed on the stator bars. In addition, using a cinestroboscopic technique a large number of high resolution pictures were taken at three different positions of the rotating disc relative to the stationary disc. These pictures were computer analyzed, statistically, to determine the fractional coverage of the bars of the stationary disc with pulp. Information obtained from these studies provides new insights into the mechanism of the refining process.

Sparsity-Based Super Resolution for SEM Images.

PubMed

Tsiper, Shahar; Dicker, Or; Kaizerman, Idan; Zohar, Zeev; Segev, Mordechai; Eldar, Yonina C

2017-09-13

The scanning electron microscope (SEM) is an electron microscope that produces an image of a sample by scanning it with a focused beam of electrons. The electrons interact with the atoms in the sample, which emit secondary electrons that contain information about the surface topography and composition. The sample is scanned by the electron beam point by point, until an image of the surface is formed. Since its invention in 1942, the capabilities of SEMs have become paramount in the discovery and understanding of the nanometer world, and today it is extensively used for both research and in industry. In principle, SEMs can achieve resolution better than one nanometer. However, for many applications, working at subnanometer resolution implies an exceedingly large number of scanning points. For exactly this reason, the SEM diagnostics of microelectronic chips is performed either at high resolution (HR) over a small area or at low resolution (LR) while capturing a larger portion of the chip. Here, we employ sparse coding and dictionary learning to algorithmically enhance low-resolution SEM images of microelectronic chips-up to the level of the HR images acquired by slow SEM scans, while considerably reducing the noise. Our methodology consists of two steps: an offline stage of learning a joint dictionary from a sequence of LR and HR images of the same region in the chip, followed by a fast-online super-resolution step where the resolution of a new LR image is enhanced. We provide several examples with typical chips used in the microelectronics industry, as well as a statistical study on arbitrary images with characteristic structural features. Conceptually, our method works well when the images have similar characteristics, as microelectronics chips do. This work demonstrates that employing sparsity concepts can greatly improve the performance of SEM, thereby considerably increasing the scanning throughput without compromising on analysis quality and resolution.

A comparison of EEOC closures involving hiring versus other prevalent discrimination issues under the Americans with Disabilities Act.

PubMed

McMahon, Brian T; Hurley, Jessica E; West, Steven L; Chan, Fong; Roessler, Richard; Rumrill, Phillip D

2008-06-01

This article describes findings from a causal comparative study of the Merit Resolution rate for allegations of Hiring discrimination that were filed with the U.S. Equal Employment Opportunity Commission (EEOC) under Title I of the Americans with Disabilities Act (ADA) between 1992 and 2005. An allegation is the Charging Party's perception of discrimination, but a Merit Resolution is one in which the EEOC has determined that a discriminatory event did indeed occur. A Non-Merit Resolution is an allegation that is closed due to a technicality or lacks sufficient evidence to conclude that discrimination occurred. Merit favors the Charging Party; Non-Merit favors the Employer. The Merit Resolution rate of 19,527 closed Hiring allegations is compared and contrasted to that of 259,680 allegations aggregated from six other prevalent forms of discrimination including Discharge and Constructive Discharge, Reasonable Accommodation, Disability Harassment and Intimidation, and Terms and Conditions of Employment. Tests of Proportion distributed as chi-square are used to form comparisons along a variety of subcategories of Merit and Non-Merit outcomes. The overall Merit Resolution rate for Hiring is 26% compared to Non-Hiring at 20.6%. Employers are less likely to settle claims of hiring discrimination without mediation, and less likely to accept the remedies recommended by the EEOC when hiring discrimination has been determined. Hiring is not an unusual discrimination issue in that the overwhelming majority of allegations are still closed in favor of the Employer. However, it is counterintuitive that hiring has a higher merit resolution rate than other prevalent issues. This finding contradicts the assumption that hiring is an "invisible process." Considering that the EEOC makes merit determinations at a competitive rate, it is clear that hiring is sufficiently transparent.

A versatile indirect detector design for hard X-ray microimaging

NASA Astrophysics Data System (ADS)

Douissard, P.-A.; Cecilia, A.; Rochet, X.; Chapel, X.; Martin, T.; van de Kamp, T.; Helfen, L.; Baumbach, T.; Luquot, L.; Xiao, X.; Meinhardt, J.; Rack, A.

2012-09-01

Indirect X-ray detectors are of outstanding importance for high resolution imaging, especially at synchrotron light sources: while consisting mostly of components which are widely commercially available, they allow for a broad range of applications in terms of the X-ray energy employed, radiation dose to the detector, data acquisition rate and spatial resolving power. Frequently, an indirect detector consists of a thin-film single crystal scintillator and a high-resolution visible light microscope as well as a camera. In this article, a novel modular-based indirect design is introduced, which offers several advantages: it can be adapted for different cameras, i.e. different sensor sizes, and can be trimmed to work either with (quasi-)monochromatic illumination and the correspondingly lower absorbed dose or with intense white beam irradiation. In addition, it allows for a motorized quick exchange between different magnifications / spatial resolutions. Developed within the European project SCINTAX, it is now commercially available. The characteristics of the detector in its different configurations (i.e. for low dose or for high dose irradiation) as measured within the SCINTAX project will be outlined. Together with selected applications from materials research, non-destructive evaluation and life sciences they underline the potential of this design to make high resolution X-ray imaging widely available.

Low-temperature high-Z gamma-detectors with very high energy resolution

NASA Astrophysics Data System (ADS)

Pobes, Carlos; Brofferio, Chiara; Bucci, Carlo; Cremonesi, Oliviero; Fiorini, Ettore; Giuliani, Andrea; Nucciotti, Angelo; Pavan, Maura; Pedretti, Marisa; Pessina, Gianluigi; Pirro, Stefano; Previtali, Ezio; Sisti, Monica; Vanzini, Marco; Zanotti, Luigi

2001-12-01

High-Z low-temperature calorimeters are developed by an Italian collaboration (Milano-Como-Gran Sasso Underground Laboratories) in order to search for rare nuclear events and Dark Matter massive candidates. They exhibit an excellent energy resolution, close to that of Ge-diodes, but a much higher efficiency. Different high-Z materials were initially employed . A many-years optimisation work on tellurium oxide (TeO2) lead to impressive results: devices with total masses around 750 g present FWHM energy resolutions on gamma-ray peaks ranging from 1 KeV (close to the 5 KeV energy threshold) to 2.6 KeV at 2615 KeV (208Tl gamma line). A 3.2 KeV FWHM energy resolution was obtained at 5.4 MeV (210Po alpha line), which is by far the best one ever achieved with any alpha detector. These devices, operated at about 10 mK, consist of a TeO2 single crystal thermally coupled to a 50 mg Neutron Transmutation Doped (NTD) Ge crystal working as a temperature sensor. Special care was devoted to methods for response linearization and temporal stabilisation. Devices based on the same principle and specifically optimised could find applications in several fields like gamma-ray astrophysics, nuclear physics searches, environmental monitoring and radiation metrology.

High Sensitivity MEMS Strain Sensor: Design and Simulation

PubMed Central

Mohammed, Ahmed A. S.; Moussa, Walied A.; Lou, Edmond

2008-01-01

In this article, we report on the new design of a miniaturized strain microsensor. The proposed sensor utilizes the piezoresistive properties of doped single crystal silicon. Employing the Micro Electro Mechanical Systems (MEMS) technology, high sensor sensitivities and resolutions have been achieved. The current sensor design employs different levels of signal amplifications. These amplifications include geometric, material and electronic levels. The sensor and the electronic circuits can be integrated on a single chip, and packaged as a small functional unit. The sensor converts input strain to resistance change, which can be transformed to bridge imbalance voltage. An analog output that demonstrates high sensitivity (0.03mV/με), high absolute resolution (1με) and low power consumption (100μA) with a maximum range of ±4000με has been reported. These performance characteristics have been achieved with high signal stability over a wide temperature range (±50°C), which introduces the proposed MEMS strain sensor as a strong candidate for wireless strain sensing applications under harsh environmental conditions. Moreover, this sensor has been designed, verified and can be easily modified to measure other values such as force, torque…etc. In this work, the sensor design is achieved using Finite Element Method (FEM) with the application of the piezoresistivity theory. This design process and the microfabrication process flow to prototype the design have been presented. PMID:27879841

Tailoring fiber grating sensors for assessment of highly refractive fuels.

PubMed

Kawano, Marianne Sumie; Heidemann, Bárbara Rutyna; Cardoso, Tárik Kaiel Machado; Possetti, Gustavo Rafael Collere; Kamikawachi, Ricardo Canute; Muller, Marcia; Fabris, José Luís

2012-04-20

Three approaches that allow the tailoring of long period gratings based refractometric sensors for concentration measurement in fuel blends are employed to assess the fuel quality in biodiesel and biodiesel-petrodiesel blend. To allow the analysis of fuel samples with refractive index higher than fiber cladding one, the samples refractive indices were changed by thermo-optic effect and by dilution in a standard substance with low refractive index. The obtained results show the sensor can detect oil concentration in biodiesel samples with resolution as better as 0.07% and biodiesel concentration in biodiesel-petrodiesel samples with average resolution of 0.09%.

Rise time measurement for ultrafast X-ray pulses

DOEpatents

Celliers, Peter M [Berkeley, CA; Weber, Franz A [Oakland, CA; Moon, Stephen J [Tracy, CA

2005-04-05

A pump-probe scheme measures the rise time of ultrafast x-ray pulses. Conventional high speed x-ray diagnostics (x-ray streak cameras, PIN diodes, diamond PCD devices) do not provide sufficient time resolution to resolve rise times of x-ray pulses on the order of 50 fs or less as they are being produced by modern fast x-ray sources. Here, we are describing a pump-probe technique that can be employed to measure events where detector resolution is insufficient to resolve the event. The scheme utilizes a diamond plate as an x-ray transducer and a p-polarized probe beam.

Rise Time Measurement for Ultrafast X-Ray Pulses

DOEpatents

Celliers, Peter M.; Weber, Franz A.; Moon, Stephen J.

2005-04-05

A pump-probe scheme measures the rise time of ultrafast x-ray pulses. Conventional high speed x-ray diagnostics (x-ray streak cameras, PIN diodes, diamond PCD devices) do not provide sufficient time resolution to resolve rise times of x-ray pulses on the order of 50 fs or less as they are being produced by modern fast x-ray sources. Here, we are describing a pump-probe technique that can be employed to measure events where detector resolution is insufficient to resolve the event. The scheme utilizes a diamond plate as an x-ray transducer and a p-polarized probe beam.

Print-to-pattern dry film photoresist lithography

NASA Astrophysics Data System (ADS)

Garland, Shaun P.; Murphy, Terrence M., Jr.; Pan, Tingrui

2014-05-01

Here we present facile microfabrication processes, referred to as print-to-pattern dry film photoresist (DFP) lithography, that utilize the combined advantages of wax printing and DFP to produce micropatterned substrates with high resolution over a large surface area in a non-cleanroom setting. The print-to-pattern methods can be performed in an out-of-cleanroom environment making microfabrication much more accessible to minimally equipped laboratories. Two different approaches employing either wax photomasks or wax etchmasks from a solid ink desktop printer have been demonstrated that allow the DFP to be processed in a negative tone or positive tone fashion, respectively, with resolutions of 100 µm. The effect of wax melting on resolution and as a bonding material was also characterized. In addition, solid ink printers have the capacity to pattern large areas with high resolution, which was demonstrated by stacking DFP layers in a 50 mm × 50 mm woven pattern with 1 mm features. By using an office printer to generate the masking patterns, the mask designs can be easily altered in a graphic user interface to enable rapid prototyping.

Direct Observation of Individual Charges and Their Dynamics on Graphene by Low-Energy Electron Holography.

PubMed

Latychevskaia, Tatiana; Wicki, Flavio; Longchamp, Jean-Nicolas; Escher, Conrad; Fink, Hans-Werner

2016-09-14

Visualizing individual charges confined to molecules and observing their dynamics with high spatial resolution is a challenge for advancing various fields in science, ranging from mesoscopic physics to electron transfer events in biological molecules. We show here that the high sensitivity of low-energy electrons to local electric fields can be employed to directly visualize individual charged adsorbates and to study their behavior in a quantitative way. This makes electron holography a unique probing tool for directly visualizing charge distributions with a sensitivity of a fraction of an elementary charge. Moreover, spatial resolution in the nanometer range and fast data acquisition inherent to lens-less low-energy electron holography allows for direct visual inspection of charge transfer processes.

Astronomical near-infrared echelle gratings

NASA Astrophysics Data System (ADS)

Hinkle, Kenneth H.; Joyce, Richard R.; Liang, Ming

2014-07-01

High-resolution near-infrared echelle spectrographs require coarse rulings in order to match the free spectral range to the detector size. Standard near-IR detector arrays typically are 2 K x 2 K or 4 K x 4 K. Detectors of this size combined with resolutions in the range 30000 to 100000 require grating groove spacings in the range 5 to 20 lines/mm. Moderately high blaze angles are desirable to reduce instrument size. Echelle gratings with these characteristics have potential wide application in both ambient temperature and cryogenic astronomical echelle spectrographs. We discuss optical designs for spectrographs employing immersed and reflective echelle gratings. The optical designs set constraints on grating characteristics. We report on market choices for obtaining these gratings and review our experiments with custom diamond turned rulings.

a New Object-Based Framework to Detect Shodows in High-Resolution Satellite Imagery Over Urban Areas

NASA Astrophysics Data System (ADS)

Tatar, N.; Saadatseresht, M.; Arefi, H.; Hadavand, A.

2015-12-01

In this paper a new object-based framework to detect shadow areas in high resolution satellite images is proposed. To produce shadow map in pixel level state of the art supervised machine learning algorithms are employed. Automatic ground truth generation based on Otsu thresholding on shadow and non-shadow indices is used to train the classifiers. It is followed by segmenting the image scene and create image objects. To detect shadow objects, a majority voting on pixel-based shadow detection result is designed. GeoEye-1 multi-spectral image over an urban area in Qom city of Iran is used in the experiments. Results shows the superiority of our proposed method over traditional pixel-based, visually and quantitatively.

High spatial resolution grain orientation and strain mapping in thin films using polychromatic submicron x-ray diffraction

NASA Astrophysics Data System (ADS)

Tamura, N.; MacDowell, A. A.; Celestre, R. S.; Padmore, H. A.; Valek, B.; Bravman, J. C.; Spolenak, R.; Brown, W. L.; Marieb, T.; Fujimoto, H.; Batterman, B. W.; Patel, J. R.

2002-05-01

The availability of high brilliance synchrotron sources, coupled with recent progress in achromatic focusing optics and large area two-dimensional detector technology, has allowed us to develop an x-ray synchrotron technique that is capable of mapping orientation and strain/stress in polycrystalline thin films with submicron spatial resolution. To demonstrate the capabilities of this instrument, we have employed it to study the microstructure of aluminum thin film structures at the granular and subgranular levels. Due to the relatively low absorption of x-rays in materials, this technique can be used to study passivated samples, an important advantage over most electron probes given the very different mechanical behavior of buried and unpassivated materials.

High-spin structures in 132Xe and 133Xe and evidence for isomers along the N =79 isotones

NASA Astrophysics Data System (ADS)

Vogt, A.; Siciliano, M.; Birkenbach, B.; Reiter, P.; Hadyńska-Klek, K.; Wheldon, C.; Valiente-Dobón, J. J.; Teruya, E.; Yoshinaga, N.; Arnswald, K.; Bazzacco, D.; Blazhev, A.; Bracco, A.; Bruyneel, B.; Chakrawarthy, R. S.; Chapman, R.; Cline, D.; Corradi, L.; Crespi, F. C. L.; Cromaz, M.; de Angelis, G.; Eberth, J.; Fallon, P.; Farnea, E.; Fioretto, E.; Fransen, C.; Freeman, S. J.; Fu, B.; Gadea, A.; Gelletly, W.; Giaz, A.; Görgen, A.; Gottardo, A.; Hayes, A. B.; Hess, H.; Hetzenegger, R.; Hirsch, R.; Hua, H.; John, P. R.; Jolie, J.; Jungclaus, A.; Karayonchev, V.; Kaya, L.; Korten, W.; Lee, I. Y.; Leoni, S.; Liang, X.; Lunardi, S.; Macchiavelli, A. O.; Menegazzo, R.; Mengoni, D.; Michelagnoli, C.; Mijatović, T.; Montagnoli, G.; Montanari, D.; Müller-Gatermann, C.; Napoli, D.; Pearson, C. J.; Podolyák, Zs.; Pollarolo, G.; Pullia, A.; Queiser, M.; Recchia, F.; Regan, P. H.; Régis, J.-M.; Saed-Samii, N.; Şahin, E.; Scarlassara, F.; Seidlitz, M.; Siebeck, B.; Sletten, G.; Smith, J. F.; Söderström, P.-A.; Stefanini, A. M.; Stezowski, O.; Szilner, S.; Szpak, B.; Teng, R.; Ur, C.; Warner, D. D.; Wolf, K.; Wu, C. Y.; Zell, K. O.

2017-08-01

The transitional nuclei 132Xe and 133Xe are investigated after multinucleon-transfer (MNT) and fusion-evaporation reactions. Both nuclei are populated (i) in 136Xe+208Pb MNT reactions employing the high-resolution Advanced GAmma Tracking Array (AGATA) coupled to the magnetic spectrometer PRISMA, (ii) in the 136Xe+198Pt MNT reaction employing the GAMMASPHERE spectrometer in combination with the gas-detector array CHICO, and (iii) as an evaporation residue after a 130Te(α ,x n )134 -x nXe fusion-evaporation reaction employing the HORUS γ -ray array at the University of Cologne. The high-spin level schemes are considerably extended above the Jπ=(7-) and (10+) isomers in 132Xe and above the 11 /2- isomer in 133Xe. The results are compared to the high-spin systematics of the Z =54 as well as the N =78 and N =79 chains. Furthermore, evidence is found for a long-lived (T1 /2≫1 μ s ) isomer in 133Xe which closes a gap along the N =79 isotones. Shell-model calculations employing the SN100PN and PQM130 effective interactions reproduce the experimental findings and provide guidance to the interpretation of the observed high-spin features.

High-spin structures in Xe 132 and Xe 133 and evidence for isomers along the N = 79 isotones

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vogt, A.; Siciliano, M.; Birkenbach, B.

In this study, the transitional nuclei 132Xe and 133Xe are investigated after multinucleon-transfer (MNT) and fusion-evaporation reactions. Both nuclei are populated (i) in 136Xe + 208Pb MNT reactions employing the high-resolution Advanced GAmma Tracking Array (AGATA) coupled to the magnetic spectrometer PRISMA, (ii) in the 136Xe + 198Pt MNT reaction employing the GAMMASPHERE spectrometer in combination with the gas-detector array CHICO, and (iii) as an evaporation residue after a 130Te (α,xn) 134-xnXe fusion-evaporation reaction employing the HORUS γ -ray array at the University of Cologne. The high-spin level schemes are considerably extended above the J π = (7 -) andmore » (10 +) isomers in 132Xe and above the 11/2 - isomer in 133Xe. The results are compared to the high-spin systematics of the Z = 54 as well as the N = 78 and N = 79 chains. Furthermore, evidence is found for a long-lived (T 1/2 » 1 μs) isomer in 133Xe which closes a gap along the N = 79 isotones. Finally, shell-model calculations employing the SN100PN and PQM130 effective interactions reproduce the experimental findings and provide guidance to the interpretation of the observed high-spin features.« less

High-spin structures in Xe 132 and Xe 133 and evidence for isomers along the N = 79 isotones

DOE PAGES

Vogt, A.; Siciliano, M.; Birkenbach, B.; ...

2017-08-24

In this study, the transitional nuclei 132Xe and 133Xe are investigated after multinucleon-transfer (MNT) and fusion-evaporation reactions. Both nuclei are populated (i) in 136Xe + 208Pb MNT reactions employing the high-resolution Advanced GAmma Tracking Array (AGATA) coupled to the magnetic spectrometer PRISMA, (ii) in the 136Xe + 198Pt MNT reaction employing the GAMMASPHERE spectrometer in combination with the gas-detector array CHICO, and (iii) as an evaporation residue after a 130Te (α,xn) 134-xnXe fusion-evaporation reaction employing the HORUS γ -ray array at the University of Cologne. The high-spin level schemes are considerably extended above the J π = (7 -) andmore » (10 +) isomers in 132Xe and above the 11/2 - isomer in 133Xe. The results are compared to the high-spin systematics of the Z = 54 as well as the N = 78 and N = 79 chains. Furthermore, evidence is found for a long-lived (T 1/2 » 1 μs) isomer in 133Xe which closes a gap along the N = 79 isotones. Finally, shell-model calculations employing the SN100PN and PQM130 effective interactions reproduce the experimental findings and provide guidance to the interpretation of the observed high-spin features.« less

Tree growth prediction using size and exposed crown area

Treesearch

Peter H. Wyckoff; James S. Clark

2005-01-01

We address the relationships between tree growth rate and growing environment for 21 co-occurring species. Tree growth rates are obtained from mapped plots at the Coweeta Long-Term Ecological Research site in the southern Appalachian Mountains. We employ high-resolution aerial photography to assess the light environment for trees growing in these plots, using exposed...

Optical system for high resolution spectrometer/monochromator

DOEpatents

Hettrick, M.C.; Underwood, J.H.

1988-10-11

An optical system for use in a spectrometer or monochromator employing a mirror which reflects electromagnetic radiation from a source to converge with same in a plane is disclosed. A straight grooved, varied-spaced diffraction grating receives the converging electromagnetic radiation from the mirror and produces a spectral image for capture by a detector, target or like receiver. 11 figs.

Genome Comparisons Reveal a Dominant Mechanism of Chromosome Number Reduction in Grasses and Accelerated Genome Evolution in Triticeae

USDA-ARS?s Scientific Manuscript database

Single nucleotide polymorphism was employed in the construction of a high-resolution, expressed sequence tag (EST) map of Aegilops tauschii, the diploid source of the wheat D genome. Comparison of the map with the rice and sorghum genome sequences revealed 50 inversions and translocations; 2, 8, and...

Bondi or not Bondi: the impact of resolution on accretion and drag force modelling for supermassive black holes

NASA Astrophysics Data System (ADS)

Beckmann, R. S.; Slyz, A.; Devriendt, J.

2018-07-01

Whilst in galaxy-size simulations, supermassive black holes (SMBHs) are entirely handled by sub-grid algorithms, computational power now allows the accretion radius of such objects to be resolved in smaller scale simulations. In this paper, we investigate the impact of resolution on two commonly used SMBH sub-grid algorithms; the Bondi-Hoyle-Lyttleton (BHL) formula for accretion on to a point mass, and the related estimate of the drag force exerted on to a point mass by a gaseous medium. We find that when the accretion region around the black hole scales with resolution, and the BHL formula is evaluated using local mass-averaged quantities, the accretion algorithm smoothly transitions from the analytic BHL formula (at low resolution) to a supply-limited accretion scheme (at high resolution). However, when a similar procedure is employed to estimate the drag force, it can lead to significant errors in its magnitude, and/or apply this force in the wrong direction in highly resolved simulations. At high Mach numbers and for small accretors, we also find evidence of the advective-acoustic instability operating in the adiabatic case, and of an instability developing around the wake's stagnation point in the quasi-isothermal case. Moreover, at very high resolution, and Mach numbers above M_∞ ≥ 3, the flow behind the accretion bow shock becomes entirely dominated by these instabilities. As a result, accretion rates on to the black hole drop by about an order of magnitude in the adiabatic case, compared to the analytic BHL formula.

High-Resolution Mesoscale Simulations of the 6-7 May 2000 Missouri Flash Flood: Impact of Model Initialization and Land Surface Treatment

NASA Technical Reports Server (NTRS)

Baker, R. David; Wang, Yansen; Tao, Wei-Kuo; Wetzel, Peter; Belcher, Larry R.

2004-01-01

High-resolution mesoscale model simulations of the 6-7 May 2000 Missouri flash flood event were performed to test the impact of model initialization and land surface treatment on timing, intensity, and location of extreme precipitation. In this flash flood event, a mesoscale convective system (MCS) produced over 340 mm of rain in roughly 9 hours in some locations. Two different types of model initialization were employed: 1) NCEP global reanalysis with 2.5-degree grid spacing and 12-hour temporal resolution, and 2) Eta reanalysis with 40- km grid spacing and $hour temporal resolution. In addition, two different land surface treatments were considered. A simple land scheme. (SLAB) keeps soil moisture fixed at initial values throughout the simulation, while a more sophisticated land model (PLACE) allows for r interactive feedback. Simulations with high-resolution Eta model initialization show considerable improvement in the intensity of precipitation due to the presence in the initialization of a residual mesoscale convective vortex (hlCV) from a previous MCS. Simulations with the PLACE land model show improved location of heavy precipitation. Since soil moisture can vary over time in the PLACE model, surface energy fluxes exhibit strong spatial gradients. These surface energy flux gradients help produce a strong low-level jet (LLJ) in the correct location. The LLJ then interacts with the cold outflow boundary of the MCS to produce new convective cells. The simulation with both high-resolution model initialization and time-varying soil moisture test reproduces the intensity and location of observed rainfall.

Bondi or not Bondi: the impact of resolution on accretion and drag force modelling for Supermassive Black Holes

NASA Astrophysics Data System (ADS)

Beckmann, R. S.; Slyz, A.; Devriendt, J.

2018-04-01

Whilst in galaxy-size simulations, supermassive black holes (SMBH) are entirely handled by sub-grid algorithms, computational power now allows the accretion radius of such objects to be resolved in smaller scale simulations. In this paper, we investigate the impact of resolution on two commonly used SMBH sub-grid algorithms; the Bondi-Hoyle-Lyttleton (BHL) formula for accretion onto a point mass, and the related estimate of the drag force exerted onto a point mass by a gaseous medium. We find that when the accretion region around the black hole scales with resolution, and the BHL formula is evaluated using local mass-averaged quantities, the accretion algorithm smoothly transitions from the analytic BHL formula (at low resolution) to a supply limited accretion (SLA) scheme (at high resolution). However, when a similar procedure is employed to estimate the drag force it can lead to significant errors in its magnitude, and/or apply this force in the wrong direction in highly resolved simulations. At high Mach numbers and for small accretors, we also find evidence of the advective-acoustic instability operating in the adiabatic case, and of an instability developing around the wake's stagnation point in the quasi-isothermal case. Moreover, at very high resolution, and Mach numbers above M_∞ ≥ 3, the flow behind the accretion bow shock becomes entirely dominated by these instabilities. As a result, accretion rates onto the black hole drop by about an order of magnitude in the adiabatic case, compared to the analytic BHL formula.

Detailed climate-change projections for urban land-use change and green-house gas increases for Belgium with COSMO-CLM coupled to TERRA_URB

NASA Astrophysics Data System (ADS)

Wouters, Hendrik; Vanden Broucke, Sam; van Lipzig, Nicole; Demuzere, Matthias

2016-04-01

Recent research clearly show that climate modelling at high resolution - which resolve the deep convection, the detailed orography and land-use including urbanization - leads to better modelling performance with respect to temperatures, the boundary-layer, clouds and precipitation. The increasing computational power enables the climate research community to address climate-change projections with higher accuracy and much more detail. In the framework of the CORDEX.be project aiming for coherent high-resolution micro-ensemble projections for Belgium employing different GCMs and RCMs, the KU Leuven contributes by means of the downscaling of EC-EARTH global climate model projections (provided by the Royal Meteorological Institute of the Netherlands) to the Belgian domain. The downscaling is obtained with regional climate simulations at 12.5km resolution over Europe (CORDEX-EU domain) and at 2.8km resolution over Belgium (CORDEX.be domain) using COSMO-CLM coupled to urban land-surface parametrization TERRA_URB. This is done for the present-day (1975-2005) and future (2040 → 2070 and 2070 → 2100). In these high-resolution runs, both GHG changes (in accordance to RCP8.5) and urban land-use changes (in accordance to a business-as-usual urban expansion scenario) are taken into account. Based on these simulations, it is shown how climate-change statistics are modified when going from coarse resolution modelling to high-resolution modelling. The climate-change statistics of particular interest are the changes in number of extreme precipitation events and extreme heat waves in cities. Hereby, it is futher investigated for the robustness of the signal change between the course and high-resolution and whether a (statistical) translation is possible. The different simulations also allow to address the relative impact and synergy between the urban expansion and increased GHG on the climate-change statistics. Hereby, it is investigated for which climate-change statistics the urban heat island and urban expansion is relevant, and to what extent the urban expansion can be included in the coarse-to-high resolution translation.

Towards high-resolution neutron imaging on IMAT

NASA Astrophysics Data System (ADS)

Minniti, T.; Tremsin, A. S.; Vitucci, G.; Kockelmann, W.

2018-01-01

IMAT is a new cold-neutron imaging facility at the neutron spallation source ISIS at the Rutherford Appleton Laboratory, U.K.. The ISIS pulsed source enables energy-selective and energy-resolved neutron imaging via time-of-flight (TOF) techniques, which are available in addition to the white-beam neutron radiography and tomography options. A spatial resolution of about 50 μm for white-beam neutron radiography was achieved early in the IMAT commissioning phase. In this work we have made the first steps towards achieving higher spatial resolution. A white-beam radiography with 18 μm spatial resolution was achieved in this experiment. This result was possible by using the event counting neutron pixel detector based on micro-channel plates (MCP) coupled with a Timepix readout chip with 55 μm sized pixels, and by employing an event centroiding technique. The prospects for energy-selective neutron radiography for this centroiding mode are discussed.

Multiband super-resolution imaging of graded-index photonic crystal flat lens

NASA Astrophysics Data System (ADS)

Xie, Jianlan; Wang, Junzhong; Ge, Rui; Yan, Bei; Liu, Exian; Tan, Wei; Liu, Jianjun

2018-05-01

Multiband super-resolution imaging of point source is achieved by a graded-index photonic crystal flat lens. With the calculations of six bands in common photonic crystal (CPC) constructed with scatterers of different refractive indices, it can be found that the super-resolution imaging of point source can be realized by different physical mechanisms in three different bands. In the first band, the imaging of point source is based on far-field condition of spherical wave while in the second band, it is based on the negative effective refractive index and exhibiting higher imaging quality than that of the CPC. However, in the fifth band, the imaging of point source is mainly based on negative refraction of anisotropic equi-frequency surfaces. The novel method of employing different physical mechanisms to achieve multiband super-resolution imaging of point source is highly meaningful for the field of imaging.

High Efficiency, Low Distortion 3D Diffusion Tensor Imaging with Variable Density Spiral Fast Spin Echoes (3D DW VDS RARE)

PubMed Central

Frank, Lawrence R.; Jung, Youngkyoo; Inati, Souheil; Tyszka, J. Michael; Wong, Eric C.

2009-01-01

We present an acquisition and reconstruction method designed to acquire high resolution 3D fast spin echo diffusion tensor images while mitigating the major sources of artifacts in DTI - field distortions, eddy currents and motion. The resulting images, being 3D, are of high SNR, and being fast spin echoes, exhibit greatly reduced field distortions. This sequence utilizes variable density spiral acquisition gradients, which allow for the implementation of a self-navigation scheme by which both eddy current and motion artifacts are removed. The result is that high resolution 3D DTI images are produced without the need for eddy current compensating gradients or B0 field correction. In addition, a novel method for fast and accurate reconstruction of the non-Cartesian data is employed. Results are demonstrated in the brains of normal human volunteers. PMID:19778618

Evolution of Orbitrap Mass Spectrometry Instrumentation

NASA Astrophysics Data System (ADS)

Eliuk, Shannon; Makarov, Alexander

2015-07-01

We discuss the evolution of OrbitrapTM mass spectrometry (MS) from its birth in the late 1990s to its current role as one of the most prominent techniques for MS. The Orbitrap mass analyzer is the first high-performance mass analyzer that employs trapping of ions in electrostatic fields. Tight integration with the ion injection process enables the high-resolution, mass accuracy, and sensitivity that have become essential for addressing analytical needs in numerous areas of research, as well as in routine analysis. We examine three major families of instruments (related to the LTQ Orbitrap, Q Exactive, and Orbitrap Fusion mass spectrometers) in the context of their historical development over the past ten eventful years. We discuss as well future trends and perspectives of Orbitrap MS. We illustrate the compelling potential of Orbitrap-based mass spectrometers as (ultra) high-resolution platforms, not only for high-end proteomic applications, but also for routine targeted analysis.

Potential applications of a high altitude powered platform in the ocean/coastal zone community

NASA Technical Reports Server (NTRS)

Escoe, D.; Rigterink, P.; Oberholtzer, J. D.

1979-01-01

The results of a survey of the ocean/coastal zone community conducted for the NASA Wallops Flight Center to identify potential applications of a high altitude powered platform (HAPP) are presented. Such a platform would stationkeep at 70,000 feet for up to a year over a given location and make frequent high resolution observations, or serve as a regional communications link. The survey results indicate user interest among scientific researchers, operational agencies and private industry. It is felt that such a platform would combine the desirable characteristics of both geostationary satellites (wide area, frequent observation) and aircraft (high resolution). As a result a concept for an operational HAPP system in the form of a 'mesoscale geostationary satellite' system evolved. Such a system could employ many of the same technologies used in current NASA and NOAA geostationary satellite programs. A set of generalized instrument requirements for HAPP borne sensors is also presented.

Thermal degradation of cloudy apple juice phenolic constituents.

PubMed

De Paepe, D; Valkenborg, D; Coudijzer, K; Noten, B; Servaes, K; De Loose, M; Voorspoels, S; Diels, L; Van Droogenbroeck, B

2014-11-01

Although conventional thermal processing is still the most commonly used preservation technique in cloudy apple juice production, detailed knowledge on phenolic compound degradation during thermal treatment is still limited. To evaluate the extent of thermal degradation as a function of time and temperature, apple juice samples were isothermally treated during 7,200s over a temperature range of 80-145 °C. An untargeted metabolomics approach based on liquid chromatography-high resolution mass spectrometry was developed and applied with the aim to find out the most heat labile phenolic constituents in cloudy apple juice. By the use of a high resolution mass spectrometer, the high degree of in-source fragmentation, the quality of deconvolution and the employed custom-made database, it was possible to achieve a high degree of structural elucidation for the thermolabile phenolic constituents. Procyanidin subclass representatives were discovered as the most heat labile phenolic compounds of cloudy apple juice. Copyright © 2014. Published by Elsevier Ltd.

Volumetric HiLo microscopy employing an electrically tunable lens.

PubMed

Philipp, Katrin; Smolarski, André; Koukourakis, Nektarios; Fischer, Andreas; Stürmer, Moritz; Wallrabe, Ulrike; Czarske, Jürgen W

2016-06-27

Electrically tunable lenses exhibit strong potential for fast motion-free axial scanning in a variety of microscopes. However, they also lead to a degradation of the achievable resolution because of aberrations and misalignment between illumination and detection optics that are induced by the scan itself. Additionally, the typically nonlinear relation between actuation voltage and axial displacement leads to over- or under-sampled frame acquisition in most microscopic techniques because of their static depth-of-field. To overcome these limitations, we present an Adaptive-Lens-High-and-Low-frequency (AL-HiLo) microscope that enables volumetric measurements employing an electrically tunable lens. By using speckle-patterned illumination, we ensure stability against aberrations of the electrically tunable lens. Its depth-of-field can be adjusted a-posteriori and hence enables to create flexible scans, which compensates for irregular axial measurement positions. The adaptive HiLo microscope provides an axial scanning range of 1 mm with an axial resolution of about 4 μm and sub-micron lateral resolution over the full scanning range. Proof of concept measurements at home-built specimens as well as zebrafish embryos with reporter gene-driven fluorescence in the thyroid gland are shown.

High resolution in-operando microimaging of solar cells with pulsed electrically-detected magnetic resonance

NASA Astrophysics Data System (ADS)

Katz, Itai; Fehr, Matthias; Schnegg, Alexander; Lips, Klaus; Blank, Aharon

2015-02-01

The in-operando detection and high resolution spatial imaging of paramagnetic defects, impurities, and states becomes increasingly important for understanding loss mechanisms in solid-state electronic devices. Electron spin resonance (ESR), commonly employed for observing these species, cannot meet this challenge since it suffers from limited sensitivity and spatial resolution. An alternative and much more sensitive method, called electrically-detected magnetic resonance (EDMR), detects the species through their magnetic fingerprint, which can be traced in the device's electrical current. However, until now it could not obtain high resolution images in operating electronic devices. In this work, the first spatially-resolved electrically-detected magnetic resonance images (EDMRI) of paramagnetic states in an operating real-world electronic device are provided. The presented method is based on a novel microwave pulse sequence allowing for the coherent electrical detection of spin echoes in combination with powerful pulsed magnetic-field gradients. The applicability of the method is demonstrated on a device-grade 1-μm-thick amorphous silicon (a-Si:H) solar cell and an identical device that was degraded locally by an electron beam. The degraded areas with increased concentrations of paramagnetic defects lead to a local increase in recombination that is mapped by EDMRI with ∼20-μm-scale pixel resolution. The novel approach presented here can be widely used in the nondestructive in-operando three-dimensional characterization of solid-state electronic devices with a resolution potential of less than 100 nm.

High Resolution Land Surface Modeling with the next generation Land Data Assimilation Systems

NASA Astrophysics Data System (ADS)

Kumar, S. V.; Eylander, J.; Peters-Lidard, C.

2005-12-01

Knowledge of land surface processes is important to many real-world applications such as agricultural production, water resources management, and flood predication. The Air Force Weather Agency (AFWA) has provided the USDA and other customers global soil moisture and temperature data for the past 30 years using the agrometeorological data assimilation model (now called AGRMET), merging atmospheric data. Further, accurate initialization of land surface conditions has been shown to greatly influence and improve weather forecast model and seasonal-to-interannual climate predictions. The AFWA AGRMET model exploits real time precipitation observations and analyses, global forecast model and satellite data to generate global estimates of soil moisture, soil temperature and other land surface states at 48km spatial resolution. However, to truly address the land surface initialization and climate prediction problem, and to mitigate the errors introduced by the differences in spatial scales of models, representations of land surface conditions need to be developed at the same fine scales such as that of cloud resolving models. NASA's Goddard Space Flight Center has developed an offline land data assimilation system known as the Land Information System (LIS) capable of modeling land atmosphere interactions at spatial resolutions as fine as 1km. LIS provides a software architecture that integrates the use of the state of the art land surface models, data assimilation techniques, and high performance computing and data management tools. LIS also employs many high resolution surface parameters such as the NASA Earth Observing System (EOS)-era products. In this study we describe the development of a next generation high resolution land surface modeling and data assimilation system, combining the capabilities of LIS and AGRMET. We investigate the influence of high resolution land surface data and observations on the land surface conditions by comparing with the operational AGRMET outputs.

A CMOS-based high-resolution fluoroscope (HRF) detector prototype with 49.5μm pixels for use in endovascular image guided interventions (EIGI)

NASA Astrophysics Data System (ADS)

Russ, M.; Shankar, A.; Setlur Nagesh, S. V.; Ionita, C. N.; Bednarek, D. R.; Rudin, S.

2017-03-01

X-ray detectors to meet the high-resolution requirements for endovascular image-guided interventions (EIGIs) are being developed and evaluated. A new 49.5-micron pixel prototype detector is being investigated and compared to the current suite of high-resolution fluoroscopic (HRF) detectors. This detector featuring a 300-micron thick CsI(Tl) scintillator, and low electronic noise CMOS readout is designated the HRF- CMOS50. To compare the abilities of this detector with other existing high resolution detectors, a standard performance metric analysis was applied, including the determination of the modulation transfer function (MTF), noise power spectra (NPS), noise equivalent quanta (NEQ), and detective quantum efficiency (DQE) for a range of energies and exposure levels. The advantage of the smaller pixel size and reduced blurring due to the thin phosphor was exemplified when the MTF of the HRF-CMOS50 was compared to the other high resolution detectors, which utilize larger pixels, other optical designs or thicker scintillators. However, the thinner scintillator has the disadvantage of a lower quantum detective efficiency (QDE) for higher diagnostic x-ray energies. The performance of the detector as part of an imaging chain was examined by employing the generalized metrics GMTF, GNEQ, and GDQE, taking standard focal spot size and clinical imaging parameters into consideration. As expected, the disparaging effects of focal spot unsharpness, exacerbated by increasing magnification, degraded the higher-frequency performance of the HRF-CMOS50, while increasing scatter fraction diminished low-frequency performance. Nevertheless, the HRF-CMOS50 brings improved resolution capabilities for EIGIs, but would require increased sensitivity and dynamic range for future clinical application.

High-speed adaptive optics line scan confocal retinal imaging for human eye

PubMed Central

Wang, Xiaolin; Zhang, Yuhua

2017-01-01

Purpose Continuous and rapid eye movement causes significant intraframe distortion in adaptive optics high resolution retinal imaging. To minimize this artifact, we developed a high speed adaptive optics line scan confocal retinal imaging system. Methods A high speed line camera was employed to acquire retinal image and custom adaptive optics was developed to compensate the wave aberration of the human eye’s optics. The spatial resolution and signal to noise ratio were assessed in model eye and in living human eye. The improvement of imaging fidelity was estimated by reduction of intra-frame distortion of retinal images acquired in the living human eyes with frame rates at 30 frames/second (FPS), 100 FPS, and 200 FPS. Results The device produced retinal image with cellular level resolution at 200 FPS with a digitization of 512×512 pixels/frame in the living human eye. Cone photoreceptors in the central fovea and rod photoreceptors near the fovea were resolved in three human subjects in normal chorioretinal health. Compared with retinal images acquired at 30 FPS, the intra-frame distortion in images taken at 200 FPS was reduced by 50.9% to 79.7%. Conclusions We demonstrated the feasibility of acquiring high resolution retinal images in the living human eye at a speed that minimizes retinal motion artifact. This device may facilitate research involving subjects with nystagmus or unsteady fixation due to central vision loss. PMID:28257458

High-speed adaptive optics line scan confocal retinal imaging for human eye.

PubMed

Lu, Jing; Gu, Boyu; Wang, Xiaolin; Zhang, Yuhua

2017-01-01

Continuous and rapid eye movement causes significant intraframe distortion in adaptive optics high resolution retinal imaging. To minimize this artifact, we developed a high speed adaptive optics line scan confocal retinal imaging system. A high speed line camera was employed to acquire retinal image and custom adaptive optics was developed to compensate the wave aberration of the human eye's optics. The spatial resolution and signal to noise ratio were assessed in model eye and in living human eye. The improvement of imaging fidelity was estimated by reduction of intra-frame distortion of retinal images acquired in the living human eyes with frame rates at 30 frames/second (FPS), 100 FPS, and 200 FPS. The device produced retinal image with cellular level resolution at 200 FPS with a digitization of 512×512 pixels/frame in the living human eye. Cone photoreceptors in the central fovea and rod photoreceptors near the fovea were resolved in three human subjects in normal chorioretinal health. Compared with retinal images acquired at 30 FPS, the intra-frame distortion in images taken at 200 FPS was reduced by 50.9% to 79.7%. We demonstrated the feasibility of acquiring high resolution retinal images in the living human eye at a speed that minimizes retinal motion artifact. This device may facilitate research involving subjects with nystagmus or unsteady fixation due to central vision loss.

High-Resolution Characterizations of Grain Boundary Damage and Stress Corrosion Cracks in Cold-Rolled Alloy 690

NASA Astrophysics Data System (ADS)

Bruemmer, S. M.; Olszta, M. J.; Toloczko, M. B.; Thomas, L. E.

Unidirectional cold rolling has been shown to promote intergranular stress corrosion cracking (IGSCC) in alloy 690 tested in PWR primary water. High-resolution scanning (SEM) and transmission electron microscopy (TEM) have been employed to investigate the microstructural reasons for this enhanced susceptibility in two stages, first examining grain boundary damage produced by cold rolling and second by characterization of stress corrosion crack tips. The degree of permanent grain boundary damage from cold rolling was found to depend directly on the initial IG precipitate distribution. Cold rolling to high levels of reduction was discovered to produce small IG voids and cracked carbides in alloys with a high density of grain boundary carbides. For the same degree of cold rolling, alloys with few IG carbides exhibited much less permanent damage. Although this difference in grain boundary damage appears to correlate with measured SCC growth rates, crack tip examinations reveal no interaction between the preexisting voids and cracked carbides with the propagation. In many cases, these features appeared to blunt propagation of IGSCC cracks. High-resolution characterizations are described for cold-rolled alloy 690 CRDM tubing and plate materials to gain insights into IGSCC mechanisms.

Preprocessing Significantly Improves the Peptide/Protein Identification Sensitivity of High-resolution Isobarically Labeled Tandem Mass Spectrometry Data*

PubMed Central

Sheng, Quanhu; Li, Rongxia; Dai, Jie; Li, Qingrun; Su, Zhiduan; Guo, Yan; Li, Chen; Shyr, Yu; Zeng, Rong

2015-01-01

Isobaric labeling techniques coupled with high-resolution mass spectrometry have been widely employed in proteomic workflows requiring relative quantification. For each high-resolution tandem mass spectrum (MS/MS), isobaric labeling techniques can be used not only to quantify the peptide from different samples by reporter ions, but also to identify the peptide it is derived from. Because the ions related to isobaric labeling may act as noise in database searching, the MS/MS spectrum should be preprocessed before peptide or protein identification. In this article, we demonstrate that there are a lot of high-frequency, high-abundance isobaric related ions in the MS/MS spectrum, and removing isobaric related ions combined with deisotoping and deconvolution in MS/MS preprocessing procedures significantly improves the peptide/protein identification sensitivity. The user-friendly software package TurboRaw2MGF (v2.0) has been implemented for converting raw TIC data files to mascot generic format files and can be downloaded for free from https://github.com/shengqh/RCPA.Tools/releases as part of the software suite ProteomicsTools. The data have been deposited to the ProteomeXchange with identifier PXD000994. PMID:25435543

Concept of dual-resolution light field imaging using an organic photoelectric conversion film for high-resolution light field photography.

PubMed

Sugimura, Daisuke; Kobayashi, Suguru; Hamamoto, Takayuki

2017-11-01

Light field imaging is an emerging technique that is employed to realize various applications such as multi-viewpoint imaging, focal-point changing, and depth estimation. In this paper, we propose a concept of a dual-resolution light field imaging system to synthesize super-resolved multi-viewpoint images. The key novelty of this study is the use of an organic photoelectric conversion film (OPCF), which is a device that converts spectra information of incoming light within a certain wavelength range into an electrical signal (pixel value), for light field imaging. In our imaging system, we place the OPCF having the green spectral sensitivity onto the micro-lens array of the conventional light field camera. The OPCF allows us to acquire the green spectra information only at the center viewpoint with the full resolution of the image sensor. In contrast, the optical system of the light field camera in our imaging system captures the other spectra information (red and blue) at multiple viewpoints (sub-aperture images) but with low resolution. Thus, our dual-resolution light field imaging system enables us to simultaneously capture information about the target scene at a high spatial resolution as well as the direction information of the incoming light. By exploiting these advantages of our imaging system, our proposed method enables the synthesis of full-resolution multi-viewpoint images. We perform experiments using synthetic images, and the results demonstrate that our method outperforms other previous methods.

UWB Tracking Algorithms: AOA and TDOA

NASA Technical Reports Server (NTRS)

Ni, Jianjun David; Arndt, D.; Ngo, P.; Gross, J.; Refford, Melinda

2006-01-01

Ultra-Wideband (UWB) tracking prototype systems are currently under development at NASA Johnson Space Center for various applications on space exploration. For long range applications, a two-cluster Angle of Arrival (AOA) tracking method is employed for implementation of the tracking system; for close-in applications, a Time Difference of Arrival (TDOA) positioning methodology is exploited. Both AOA and TDOA are chosen to utilize the achievable fine time resolution of UWB signals. This talk presents a brief introduction to AOA and TDOA methodologies. The theoretical analysis of these two algorithms reveal the affecting parameters impact on the tracking resolution. For the AOA algorithm, simulations show that a tracking resolution less than 0.5% of the range can be achieved with the current achievable time resolution of UWB signals. For the TDOA algorithm used in close-in applications, simulations show that the (sub-inch) high tracking resolution is achieved with a chosen tracking baseline configuration. The analytical and simulated results provide insightful guidance for the UWB tracking system design.

Localization microscopy of DNA in situ using Vybrant{sup ®} DyeCycle™ Violet fluorescent probe: A new approach to study nuclear nanostructure at single molecule resolution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Żurek-Biesiada, Dominika; Szczurek, Aleksander T.; Prakash, Kirti

Higher order chromatin structure is not only required to compact and spatially arrange long chromatids within a nucleus, but have also important functional roles, including control of gene expression and DNA processing. However, studies of chromatin nanostructures cannot be performed using conventional widefield and confocal microscopy because of the limited optical resolution. Various methods of superresolution microscopy have been described to overcome this difficulty, like structured illumination and single molecule localization microscopy. We report here that the standard DNA dye Vybrant{sup ®} DyeCycle™ Violet can be used to provide single molecule localization microscopy (SMLM) images of DNA in nuclei ofmore » fixed mammalian cells. This SMLM method enabled optical isolation and localization of large numbers of DNA-bound molecules, usually in excess of 10{sup 6} signals in one cell nucleus. The technique yielded high-quality images of nuclear DNA density, revealing subdiffraction chromatin structures of the size in the order of 100 nm; the interchromatin compartment was visualized at unprecedented optical resolution. The approach offers several advantages over previously described high resolution DNA imaging methods, including high specificity, an ability to record images using a single wavelength excitation, and a higher density of single molecule signals than reported in previous SMLM studies. The method is compatible with DNA/multicolor SMLM imaging which employs simple staining methods suited also for conventional optical microscopy. - Highlights: • Super-resolution imaging of nuclear DNA with Vybrant Violet and blue excitation. • 90nm resolution images of DNA structures in optically thick eukaryotic nuclei. • Enhanced resolution confirms the existence of DNA-free regions inside the nucleus. • Optimized imaging conditions enable multicolor super-resolution imaging.« less

An approach for retrieval of atmospheric trace gases CO II, CH 4 and CO from the future Canadian micro earth observation satellite (MEOS)

NASA Astrophysics Data System (ADS)

Trishchenko, Alexander P.; Khlopenkov, Konstantin V.; Wang, Shusen; Luo, Yi; Kruzelecky, Roman V.; Jamroz, Wes; Kroupnik, Guennadi

2007-10-01

Among all trace gases, the carbon dioxide and methane provide the largest contribution to the climate radiative forcing and together with carbon monoxide also to the global atmospheric carbon budget. New Micro Earth Observation Satellite (MEOS) mission is proposed to obtain information about these gases along with some other mission's objectives related to studying cloud and aerosol interactions. The miniature suit of instruments is proposed to make measurements with reduced spectral resolution (1.2nm) over wide NIR range 0.9μm to 2.45μm and with high spectral resolution (0.03nm) for three selected regions: oxygen A-band, 1.5μm-1.7μm band and 2.2μm-2.4μm band. It is also planned to supplement the spectrometer measurements with high spatial resolution imager for detailed characterization of cloud and surface albedo distribution within spectrometer field of view. The approaches for cloud/clear-sky identification and column retrievals of above trace gases are based on differential absorption technique and employ the combination of coarse and high-resolution spectral data. The combination of high and coarse resolution spectral data is beneficial for better characterization of surface spectral albedo and aerosol effects. An additional capability for retrieval of the vertical distribution amounts is obtained from the combination of nadir and limb measurements. Oxygen A-band path length will be used for normalization of trace gas retrievals.

Fracture behavior of W based materials. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hack, J.E.

This report describes the results of a program to investigate the fracture properties of tungsten based materials. In particular, the role of crack velocity on crack instability was determined in a W-Fe-Ni-Co ``heavy alloy`` and pure polycrystalline tungsten. A considerable effort was expended on the development of an appropriate crack velocity gage for use on these materials. Having succeeded in that, the gage technology was employed to determine the crack velocity response to the applied level of stress intensity factor at the onset of crack instability in pre-cracked specimens. The results were also correlated to the failure mode observed inmore » two material systems of interest. Major results include: (1) unstable crack velocity measurements on metallic specimens which require high spatial resolution require the use of brittle, insulating substrates, as opposed to the ductile, polymer based substrates employed in low spatial resolution measurements; and (2) brittle failure modes, such as cleavage, are characterized by relatively slow unstable crack velocities while evidence of high degrees of deformation are associated with failures which proceed at high unstable crack velocities. This latter behavior is consistent with the predictions of the modeling of Hack et al and may have a significant impact on the interpretation of fractographs in general.« less

Knowledge-based nonuniform sampling in multidimensional NMR.

PubMed

Schuyler, Adam D; Maciejewski, Mark W; Arthanari, Haribabu; Hoch, Jeffrey C

2011-07-01

The full resolution afforded by high-field magnets is rarely realized in the indirect dimensions of multidimensional NMR experiments because of the time cost of uniformly sampling to long evolution times. Emerging methods utilizing nonuniform sampling (NUS) enable high resolution along indirect dimensions by sampling long evolution times without sampling at every multiple of the Nyquist sampling interval. While the earliest NUS approaches matched the decay of sampling density to the decay of the signal envelope, recent approaches based on coupled evolution times attempt to optimize sampling by choosing projection angles that increase the likelihood of resolving closely-spaced resonances. These approaches employ knowledge about chemical shifts to predict optimal projection angles, whereas prior applications of tailored sampling employed only knowledge of the decay rate. In this work we adapt the matched filter approach as a general strategy for knowledge-based nonuniform sampling that can exploit prior knowledge about chemical shifts and is not restricted to sampling projections. Based on several measures of performance, we find that exponentially weighted random sampling (envelope matched sampling) performs better than shift-based sampling (beat matched sampling). While shift-based sampling can yield small advantages in sensitivity, the gains are generally outweighed by diminished robustness. Our observation that more robust sampling schemes are only slightly less sensitive than schemes highly optimized using prior knowledge about chemical shifts has broad implications for any multidimensional NMR study employing NUS. The results derived from simulated data are demonstrated with a sample application to PfPMT, the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum.

Image Quality in High-resolution and High-cadence Solar Imaging

NASA Astrophysics Data System (ADS)

Denker, C.; Dineva, E.; Balthasar, H.; Verma, M.; Kuckein, C.; Diercke, A.; González Manrique, S. J.

2018-03-01

Broad-band imaging and even imaging with a moderate bandpass (about 1 nm) provides a photon-rich environment, where frame selection (lucky imaging) becomes a helpful tool in image restoration, allowing us to perform a cost-benefit analysis on how to design observing sequences for imaging with high spatial resolution in combination with real-time correction provided by an adaptive optics (AO) system. This study presents high-cadence (160 Hz) G-band and blue continuum image sequences obtained with the High-resolution Fast Imager (HiFI) at the 1.5-meter GREGOR solar telescope, where the speckle-masking technique is used to restore images with nearly diffraction-limited resolution. The HiFI employs two synchronized large-format and high-cadence sCMOS detectors. The median filter gradient similarity (MFGS) image-quality metric is applied, among others, to AO-corrected image sequences of a pore and a small sunspot observed on 2017 June 4 and 5. A small region of interest, which was selected for fast-imaging performance, covered these contrast-rich features and their neighborhood, which were part of Active Region NOAA 12661. Modifications of the MFGS algorithm uncover the field- and structure-dependency of this image-quality metric. However, MFGS still remains a good choice for determining image quality without a priori knowledge, which is an important characteristic when classifying the huge number of high-resolution images contained in data archives. In addition, this investigation demonstrates that a fast cadence and millisecond exposure times are still insufficient to reach the coherence time of daytime seeing. Nonetheless, the analysis shows that data acquisition rates exceeding 50 Hz are required to capture a substantial fraction of the best seeing moments, significantly boosting the performance of post-facto image restoration.

Accelerated high-resolution photoacoustic tomography via compressed sensing

NASA Astrophysics Data System (ADS)

Arridge, Simon; Beard, Paul; Betcke, Marta; Cox, Ben; Huynh, Nam; Lucka, Felix; Ogunlade, Olumide; Zhang, Edward

2016-12-01

Current 3D photoacoustic tomography (PAT) systems offer either high image quality or high frame rates but are not able to deliver high spatial and temporal resolution simultaneously, which limits their ability to image dynamic processes in living tissue (4D PAT). A particular example is the planar Fabry-Pérot (FP) photoacoustic scanner, which yields high-resolution 3D images but takes several minutes to sequentially map the incident photoacoustic field on the 2D sensor plane, point-by-point. However, as the spatio-temporal complexity of many absorbing tissue structures is rather low, the data recorded in such a conventional, regularly sampled fashion is often highly redundant. We demonstrate that combining model-based, variational image reconstruction methods using spatial sparsity constraints with the development of novel PAT acquisition systems capable of sub-sampling the acoustic wave field can dramatically increase the acquisition speed while maintaining a good spatial resolution: first, we describe and model two general spatial sub-sampling schemes. Then, we discuss how to implement them using the FP interferometer and demonstrate the potential of these novel compressed sensing PAT devices through simulated data from a realistic numerical phantom and through measured data from a dynamic experimental phantom as well as from in vivo experiments. Our results show that images with good spatial resolution and contrast can be obtained from highly sub-sampled PAT data if variational image reconstruction techniques that describe the tissues structures with suitable sparsity-constraints are used. In particular, we examine the use of total variation (TV) regularization enhanced by Bregman iterations. These novel reconstruction strategies offer new opportunities to dramatically increase the acquisition speed of photoacoustic scanners that employ point-by-point sequential scanning as well as reducing the channel count of parallelized schemes that use detector arrays.

Test target for characterizing 3D resolution of optical coherence tomography

NASA Astrophysics Data System (ADS)

Hu, Zhixiong; Hao, Bingtao; Liu, Wenli; Hong, Baoyu; Li, Jiao

2014-12-01

Optical coherence tomography (OCT) is a non-invasive 3D imaging technology which has been applied or investigated in many diagnostic fields including ophthalmology, dermatology, dentistry, cardiovasology, endoscopy, brain imaging and so on. Optical resolution is an important characteristic that can describe the quality and utility of an image acquiring system. We employ 3D printing technology to design and fabricate a test target for characterizing 3D resolution of optical coherence tomography. The test target which mimics USAF 1951 test chart was produced with photopolymer. By measuring the 3D test target, axial resolution as well as lateral resolution of a spectral domain OCT system was evaluated. For comparison, conventional microscope and surface profiler were employed to characterize the 3D test targets. The results demonstrate that the 3D resolution test targets have the potential of qualitatively and quantitatively validating the performance of OCT systems.

Stochastic Downscaling of Digital Elevation Models

NASA Astrophysics Data System (ADS)

Rasera, Luiz Gustavo; Mariethoz, Gregoire; Lane, Stuart N.

2016-04-01

High-resolution digital elevation models (HR-DEMs) are extremely important for the understanding of small-scale geomorphic processes in Alpine environments. In the last decade, remote sensing techniques have experienced a major technological evolution, enabling fast and precise acquisition of HR-DEMs. However, sensors designed to measure elevation data still feature different spatial resolution and coverage capabilities. Terrestrial altimetry allows the acquisition of HR-DEMs with centimeter to millimeter-level precision, but only within small spatial extents and often with dead ground problems. Conversely, satellite radiometric sensors are able to gather elevation measurements over large areas but with limited spatial resolution. In the present study, we propose an algorithm to downscale low-resolution satellite-based DEMs using topographic patterns extracted from HR-DEMs derived for example from ground-based and airborne altimetry. The method consists of a multiple-point geostatistical simulation technique able to generate high-resolution elevation data from low-resolution digital elevation models (LR-DEMs). Initially, two collocated DEMs with different spatial resolutions serve as an input to construct a database of topographic patterns, which is also used to infer the statistical relationships between the two scales. High-resolution elevation patterns are then retrieved from the database to downscale a LR-DEM through a stochastic simulation process. The output of the simulations are multiple equally probable DEMs with higher spatial resolution that also depict the large-scale geomorphic structures present in the original LR-DEM. As these multiple models reflect the uncertainty related to the downscaling, they can be employed to quantify the uncertainty of phenomena that are dependent on fine topography, such as catchment hydrological processes. The proposed methodology is illustrated for a case study in the Swiss Alps. A swissALTI3D HR-DEM (with 5 m resolution) and a SRTM-derived LR-DEM from the Western Alps are used to downscale a SRTM-based LR-DEM from the eastern part of the Alps. The results show that the method is capable of generating multiple high-resolution synthetic DEMs that reproduce the spatial structure and statistics of the original DEM.

20 CFR 627.481 - Audit resolution.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Audit resolution. 627.481 Section 627.481... PROGRAMS UNDER TITLES I, II, AND III OF THE ACT Administrative Standards § 627.481 Audit resolution. (a) Federal audit resolution. When the OIG issues an audit report to the Employment and Training...

Guiding Biogeochemical Campaigns with High Resolution Altimetry: Waiting for the SWOT Mission

NASA Astrophysics Data System (ADS)

d'Ovidio, Francesco; Zhou, Meng; Park, Young Hyang; Nencioli, Francesco; Resplandy, Laure; Doglioli, Andrea; Petrenko, Anne; Blain, Stephane; Queguiner, Bernard

2013-09-01

Biogeochemical processes in the ocean are strongly affected by the horizontal mesoscale ( 10-100 km) and submesoscale (1-10 km) circulation. Eddies and filaments can create strong dishomogeneity, either amplifying small-scale diffusion processes (mixing) or creating tracer reservoirs. This variability has a direct effect on the biogeochemical budgets - controlling for instances tracer fluxes across climatological fronts, or part of the vertical exchanges. This variability also provides a challenge to in situ studies, because sites few tens of kms or few weeks apart may be representative of very different situations. Here we discuss how altimetry observation can be exploited in order to track in near- real-time transport barriers and mixing regions and guide a biogeochemical adaptative sampling strategy. As a case study, we focus on the recent KEOPS2 campaign (Kerguelen region, October-November 2012) which employed Lagrangian diagnostics of a specifically designed high resolution, regional altimetric product produced by CLS (with support from CNES) analyzed with several Lagrangian diagnostics. Such approach anticipates possible uses of incoming high resolution altimetry data for biogeochemical studies.

A New Pansharpening Method Based on Spatial and Spectral Sparsity Priors.

PubMed

He, Xiyan; Condat, Laurent; Bioucas-Diaz, Jose; Chanussot, Jocelyn; Xia, Junshi

2014-06-27

The development of multisensor systems in recent years has led to great increase in the amount of available remote sensing data. Image fusion techniques aim at inferring high quality images of a given area from degraded versions of the same area obtained by multiple sensors. This paper focuses on pansharpening, which is the inference of a high spatial resolution multispectral image from two degraded versions with complementary spectral and spatial resolution characteristics: a) a low spatial resolution multispectral image; and b) a high spatial resolution panchromatic image. We introduce a new variational model based on spatial and spectral sparsity priors for the fusion. In the spectral domain we encourage low-rank structure, whereas in the spatial domain we promote sparsity on the local differences. Given the fact that both panchromatic and multispectral images are integrations of the underlying continuous spectra using different channel responses, we propose to exploit appropriate regularizations based on both spatial and spectral links between panchromatic and the fused multispectral images. A weighted version of the vector Total Variation (TV) norm of the data matrix is employed to align the spatial information of the fused image with that of the panchromatic image. With regard to spectral information, two different types of regularization are proposed to promote a soft constraint on the linear dependence between the panchromatic and the fused multispectral images. The first one estimates directly the linear coefficients from the observed panchromatic and low resolution multispectral images by Linear Regression (LR) while the second one employs the Principal Component Pursuit (PCP) to obtain a robust recovery of the underlying low-rank structure. We also show that the two regularizers are strongly related. The basic idea of both regularizers is that the fused image should have low-rank and preserve edge locations. We use a variation of the recently proposed Split Augmented Lagrangian Shrinkage (SALSA) algorithm to effectively solve the proposed variational formulations. Experimental results on simulated and real remote sensing images show the effectiveness of the proposed pansharpening method compared to the state-of-the-art.

AVAL - The ASTER Volcanic Ash Library

NASA Astrophysics Data System (ADS)

Williams, D.; Ramsey, M. S.

2016-12-01

Volcanic ash is a rich data source for understanding the causal mechanisms behind volcanic eruptions. Petrologic and morphometric information can provide direct information on the characteristics of the parent magma. Understanding how erupted ash interacts with the atmosphere can help quantify the effect that explosive volcanism has on the local to regional climate, whereas a measure of the particle size distribution enables more accurate modeling of plume propagation. Remote sensing is regularly employed to monitor volcanic plumes using a suite of high temporal/low spatial resolution sensors. These methods employ radiative transfer modeling with assumptions of the transmissive properties of infrared energy through the plume to determine ash density, particle size and sulfur dioxide content. However, such approaches are limited to the optically-transparent regions, and the low spatial resolution data are only useful for large-scale trends. In a new approach, we are treating the infrared-opaque regions of the plume in a similar way to a solid emitting surface. This allows high spatial resolution orbital thermal infrared data from the dense proximal plume to be modeled using a linear deconvolution approach coupled with a spectral library to extract the particle size and petrology. The newly created ASTER Volcanic Ash Library (AVAL) provides the end member spectral suite, and is comprised of laboratory emission measurements of volcanic ash taken from a variety of different volcanic settings, to obtain a wide range of petrologies. These samples have been further subdivided into particle size fractions to account for spectral changes due to diffraction effects. Once mapped to the ASTER sensor's spectral resolution, this library is applied to image data and the plume deconvolved to estimate composition and particle size. We have analyzed eruptions at the Soufrière Hills Volcano, Montserrat, Chaitén and Puyehue-Cordón Caulle, both Chile, and Eyjafjallajökull, Iceland. These results provide particle size distributions within actively-erupting volcanic plumes for the first time in high resolution, and the petrologic information is being studied to understand the underlying eruptive processes observed.

Multi-MHz laser-scanning single-cell fluorescence microscopy by spatiotemporally encoded virtual source array

PubMed Central

Wu, Jianglai; Tang, Anson H. L.; Mok, Aaron T. Y.; Yan, Wenwei; Chan, Godfrey C. F.; Wong, Kenneth K. Y.; Tsia, Kevin K.

2017-01-01

Apart from the spatial resolution enhancement, scaling of temporal resolution, equivalently the imaging throughput, of fluorescence microscopy is of equal importance in advancing cell biology and clinical diagnostics. Yet, this attribute has mostly been overlooked because of the inherent speed limitation of existing imaging strategies. To address the challenge, we employ an all-optical laser-scanning mechanism, enabled by an array of reconfigurable spatiotemporally-encoded virtual sources, to demonstrate ultrafast fluorescence microscopy at line-scan rate as high as 8 MHz. We show that this technique enables high-throughput single-cell microfluidic fluorescence imaging at 75,000 cells/second and high-speed cellular 2D dynamical imaging at 3,000 frames per second, outperforming the state-of-the-art high-speed cameras and the gold-standard laser scanning strategies. Together with its wide compatibility to the existing imaging modalities, this technology could empower new forms of high-throughput and high-speed biological fluorescence microscopy that was once challenged. PMID:28966855

Spatial resolution study and power calibration of the high-k scattering system on NSTX.

PubMed

Lee, W; Park, H K; Cho, M H; Namkung, W; Smith, D R; Domier, C W; Luhmann, N C

2008-10-01

NSTX high-k scattering system has been extensively utilized in studying the microturbulence and coherent waves. An absolute calibration of the scattering system was performed employing a new millimeter-wave source and calibrated attenuators. One of the key parameters essential for the calibration of the multichannel scattering system is the interaction length. This interaction length is significantly different from the conventional one due to the curvature and magnetic shear effect.

Object oriented classification of high resolution data for inventory of horticultural crops

NASA Astrophysics Data System (ADS)

Hebbar, R.; Ravishankar, H. M.; Trivedi, S.; Subramoniam, S. R.; Uday, R.; Dadhwal, V. K.

2014-11-01

High resolution satellite images are associated with large variance and thus, per pixel classifiers often result in poor accuracy especially in delineation of horticultural crops. In this context, object oriented techniques are powerful and promising methods for classification. In the present study, a semi-automatic object oriented feature extraction model has been used for delineation of horticultural fruit and plantation crops using Erdas Objective Imagine. Multi-resolution data from Resourcesat LISS-IV and Cartosat-1 have been used as source data in the feature extraction model. Spectral and textural information along with NDVI were used as inputs for generation of Spectral Feature Probability (SFP) layers using sample training pixels. The SFP layers were then converted into raster objects using threshold and clump function resulting in pixel probability layer. A set of raster and vector operators was employed in the subsequent steps for generating thematic layer in the vector format. This semi-automatic feature extraction model was employed for classification of major fruit and plantations crops viz., mango, banana, citrus, coffee and coconut grown under different agro-climatic conditions. In general, the classification accuracy of about 75-80 per cent was achieved for these crops using object based classification alone and the same was further improved using minimal visual editing of misclassified areas. A comparison of on-screen visual interpretation with object oriented approach showed good agreement. It was observed that old and mature plantations were classified more accurately while young and recently planted ones (3 years or less) showed poor classification accuracy due to mixed spectral signature, wider spacing and poor stands of plantations. The results indicated the potential use of object oriented approach for classification of high resolution data for delineation of horticultural fruit and plantation crops. The present methodology is applicable at local levels and future development is focused on up-scaling the methodology for generation of fruit and plantation crop maps at regional and national level which is important for creation of database for overall horticultural crop development.

Analysis of embolic signals with directional dual tree rational dilation wavelet transform.

PubMed

Serbes, Gorkem; Aydin, Nizamettin

2016-08-01

The dyadic discrete wavelet transform (dyadic-DWT), which is based on fixed integer sampling factor, has been used before for processing piecewise smooth biomedical signals. However, the dyadic-DWT has poor frequency resolution due to the low-oscillatory nature of its wavelet bases and therefore, it is less effective in processing embolic signals (ESs). To process ESs more effectively, a wavelet transform having better frequency resolution than the dyadic-DWT is needed. Therefore, in this study two ESs, containing micro-emboli and artifact waveforms, are analyzed with the Directional Dual Tree Rational-Dilation Wavelet Transform (DDT-RADWT). The DDT-RADWT, which can be directly applied to quadrature signals, is based on rational dilation factors and has adjustable frequency resolution. The analyses are done for both low and high Q-factors. It is proved that, when high Q-factor filters are employed in the DDT-RADWT, clearer representations of ESs can be attained in decomposed sub-bands and artifacts can be successfully separated.

Dynamic-Receive Focusing with High-Frequency Annular Arrays

NASA Astrophysics Data System (ADS)

Ketterling, J. A.; Mamou, J.; Silverman, R. H.

High-frequency ultrasound is commonly employed for ophthalmic and small-animal imaging because of the fine-resolution images it affords. Annular arrays allow improved depth of field and lateral resolution versus commonly used single-element, focused transducers. The best image quality from an annular array is achieved by using synthetic transmit-to-receive focusing while utilizing data from all transmit-to-receive element combinations. However, annular arrays must be laterally scanned to form an image and this requires one pass for each of the array elements when implementing full synthetic transmit-to-receive focusing. A dynamic-receive focusing approach permits a single pass, although at a sacrifice of depth of field and lateral resolution. A five-element, 20-MHz annular array is examined to determine the acoustic beam properties for synthetic and dynamic-receive focusing. A spatial impulse response model is used to simulate the acoustic beam properties for each focusing case and then data acquired from a human eye-bank eye are processed to demonstrate the effect of each approach on image quality.

Monitoring tumor growth and treatment in small animals with magnetic resonance and optical tomographic imaging

NASA Astrophysics Data System (ADS)

Masciotti, J.; Provenzano, F.; Papa, J.; Klose, A.; Hur, J.; Gu, X.; Yamashiro, D.; Kandel, J.; Hielscher, A. H.

2006-02-01

Small animal models are employed to simulate disease in humans and to study its progression, what factors are important to the disease process, and to study the disease treatment. Biomedical imaging modalities such as magnetic resonance imaging (MRI) and Optical Tomography make it possible to non-invasively monitor the progression of diseases in living small animals and study the efficacy of drugs and treatment protocols. MRI is an established imaging modality capable of obtaining high resolution anatomical images and along with contrast agents allow the studying of blood volume. Optical tomography, on the other hand, is an emerging imaging modality, which, while much lower in spatial resolution, can separate the effects of oxyhemoglobin, deoxyhemoglobin, and blood volume with high temporal resolution. In this study we apply these modalities to imaging the growth of kidney tumors and then there treatment by an anti-VEGF agent. We illustrate how these imaging modalities have their individual uses, but can still supplement each other and cross validation can be performed.

A YBCO RF-squid variable temperature susceptometer and its applications

NASA Technical Reports Server (NTRS)

Zhou, Luwei; Qiu, Jinwu; Zhang, Xianfeng; Tang, Zhimin; Cai, Yimin; Qian, Yongjia

1991-01-01

The Superconducting QUantum Interference Device (SQUID) susceptibility using a high-temperature radio-frequency (rf) SQUID and a normal metal pick-up coil is employed in testing weak magnetization of the sample. The magnetic moment resolution of the device is 1 x 10(exp -6) emu, and that of the susceptibility is 5 x 10(exp -6) emu/cu cm.

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

DOE PAGES

Carlton, Holly D.; Elmer, John W.; Li, Yan; ...

2016-04-13

For this study synchrotron radiation micro-­tomography, a non-destructive three-dimensional imaging technique, is employed to investigate an entire microelectronic package with a cross-sectional area of 16 x 16 mm. Due to the synchrotron’s high flux and brightness the sample was imaged in just 3 minutes with an 8.7 μm spatial resolution.

Utility of high-resolution accurate MS to eliminate interferences in the bioanalysis of ribavirin and its phosphate metabolites.

PubMed

Wei, Cong; Grace, James E; Zvyaga, Tatyana A; Drexler, Dieter M

2012-08-01

The polar nucleoside drug ribavirin (RBV) combined with IFN-α is a front-line treatment for chronic hepatitis C virus infection. RBV acts as a prodrug and exerts its broad antiviral activity primarily through its active phosphorylated metabolite ribavirin 5´-triphosphate (RTP), and also possibly through ribavirin 5´-monophosphate (RMP). To study RBV transport, diffusion, metabolic clearance and its impact on drug-metabolizing enzymes, a LC-MS method is needed to simultaneously quantify RBV and its phosphorylated metabolites (RTP, ribavirin 5´-diphosphate and RMP). In a recombinant human UGT1A1 assay, the assay buffer components uridine and its phosphorylated derivatives are isobaric with RBV and its phosphorylated metabolites, leading to significant interference when analyzed by LC-MS with the nominal mass resolution mode. Presented here is a LC-MS method employing LC coupled with full-scan high-resolution accurate MS analysis for the simultaneous quantitative determination of RBV, RMP, ribavirin 5´-diphosphate and RTP by differentiating RBV and its phosphorylated metabolites from uridine and its phosphorylated derivatives by accurate mass, thus avoiding interference. The developed LC-high-resolution accurate MS method allows for quantitation of RBV and its phosphorylated metabolites, eliminating the interferences from uridine and its phosphorylated derivatives in recombinant human UGT1A1 assays.

Zero-crossing approach to high-resolution reconstruction in frequency-domain optical-coherence tomography.

PubMed

Krishnan, Sunder Ram; Seelamantula, Chandra Sekhar; Bouwens, Arno; Leutenegger, Marcel; Lasser, Theo

2012-10-01

We address the problem of high-resolution reconstruction in frequency-domain optical-coherence tomography (FDOCT). The traditional method employed uses the inverse discrete Fourier transform, which is limited in resolution due to the Heisenberg uncertainty principle. We propose a reconstruction technique based on zero-crossing (ZC) interval analysis. The motivation for our approach lies in the observation that, for a multilayered specimen, the backscattered signal may be expressed as a sum of sinusoids, and each sinusoid manifests as a peak in the FDOCT reconstruction. The successive ZC intervals of a sinusoid exhibit high consistency, with the intervals being inversely related to the frequency of the sinusoid. The statistics of the ZC intervals are used for detecting the frequencies present in the input signal. The noise robustness of the proposed technique is improved by using a cosine-modulated filter bank for separating the input into different frequency bands, and the ZC analysis is carried out on each band separately. The design of the filter bank requires the design of a prototype, which we accomplish using a Kaiser window approach. We show that the proposed method gives good results on synthesized and experimental data. The resolution is enhanced, and noise robustness is higher compared with the standard Fourier reconstruction.

Employing X-ray absorption technique for better detector resolution and measurement of low cross-section events

NASA Astrophysics Data System (ADS)

Sharma, Gaurav; Puri, Nitin K.; Kumar, Pravin; Nandi, T.

2018-03-01

The versatility of X-ray absorption technique is experimentally employed for enhancing the detector resolution and to rejuvenate the low probable transitions obscured in the pile-up region, during a beam-foil spectroscopy experiment. The multiple aluminum absorber layers (10 μm each) are used to suppress the pile-up contribution drastically and to restore a weak transition which is about 1.38 × 104 times weaker than a one-electron-one-photon transitions viz. Kα and Khα. The weak line is possibly originating from a two-electron-one-photon transition in He-like Ti. Further, the transitions, which were obscured in the spectra due to high intensity ratio, are revived by dissimilar line intensity attenuation using this technique. The measured lifetimes of Kα line with and without intensity attenuation match well within error bar. The present technique finds potential implications in understanding the structure of multiple-core-vacant ions and other low cross section processes in ion-solid collisions.

The 2010 Eyjafjallajokull Eruptions: The NASA Applied Sciences Perspective for Aviation

NASA Astrophysics Data System (ADS)

Murray, J. J.; Haynes, J. A.; Trepte, C. R.; Krotkov, N. A.; Krueger, A. J.

2010-12-01

The volcanic ash from the eruption of the Eyjafjallajokull volcano in Iceland which began on March 17, 2010 was closely monitored by NASA Earth Observing System satellites. A wide variety of applications and techniques developed by the NASA Science Mission Directorate’s Applied Science Program were employed. These included information from imager data obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS) onboard the Aqua and Terra spacecraft. Horizontal distribution of the ash cloud and column amount of volcanic sufur dioxide gas was accurately mapped by the Ozone Monitoring Instrument (OMI) onboard the Aura satellite. Highly precise retrievals of the vertical distribution of volcanic aerosols were obtained by the Caliop instrument onboard the Calipso satellite. The Multi-angle Imaging SpectroRadiometer (MISR) satellite also provided stereo-derived plume heights at 1km horizontal and ~0.5km vertical resolutions. All of this information was employed to assist in airspace management during the eruptive period. It will continue to be used to improve dispersion models and procedures for dealing with volcanic ash.

Table-top phase-contrast imaging employing photon-counting detectors towards mammographic applications

NASA Astrophysics Data System (ADS)

Palma, K. D.; Pichotka, M.; Hasn, S.; Granja, C.

2017-02-01

In mammography the difficult task to detect microcalcifications (≈ 100 μm) and low contrast structures in the breast has been a topic of interest from its beginnings. The possibility to improve the image quality requires the effort to employ novel X-ray imaging techniques, such as phase-contrast, and high resolution detectors. Phase-contrast techniques are promising tools for medical diagnosis because they provide additional and complementary information to traditional absorption-based X-ray imaging methods. In this work a Hamamatsu microfocus X-ray source with tungsten anode and a photon counting detector (Timepix operated in Medipix mode) was used. A significant improvement in the detection of phase-effects using Medipix detector was observed in comparison to an standard flat-panel detector. An optimization of geometrical parameters reveals the dependency on the X-ray propagation path and the small angle deviation. The quantification of these effects was achieved taking into account the image noise, contrast, spatial resolution of the phase-enhancement, absorbed dose, and energy dependence.

Apparatus and method to achieve high-resolution microscopy with non-diffracting or refracting radiation

DOEpatents

Tobin, Jr., Kenneth W.; Bingham, Philip R.; Hawari, Ayman I.

2012-11-06

An imaging system employing a coded aperture mask having multiple pinholes is provided. The coded aperture mask is placed at a radiation source to pass the radiation through. The radiation impinges on, and passes through an object, which alters the radiation by absorption and/or scattering. Upon passing through the object, the radiation is detected at a detector plane to form an encoded image, which includes information on the absorption and/or scattering caused by the material and structural attributes of the object. The encoded image is decoded to provide a reconstructed image of the object. Because the coded aperture mask includes multiple pinholes, the radiation intensity is greater than a comparable system employing a single pinhole, thereby enabling a higher resolution. Further, the decoding of the encoded image can be performed to generate multiple images of the object at different distances from the detector plane. Methods and programs for operating the imaging system are also disclosed.

High resolution SAW elastography for ex-vivo porcine skin specimen

NASA Astrophysics Data System (ADS)

Zhou, Kanheng; Feng, Kairui; Wang, Mingkai; Jamera, Tanatswa; Li, Chunhui; Huang, Zhihong

2018-02-01

Surface acoustic wave (SAW) elastography has been proven to be a non-invasive, non-destructive method for accurately characterizing tissue elastic properties. Current SAW elastography technique tracks generated surface acoustic wave impulse point by point which are a few millimeters away. Thus, reconstructed elastography has low lateral resolution. To improve the lateral resolution of current SAW elastography, a new method was proposed in this research. A M-B scan mode, high spatial resolution phase sensitive optical coherence tomography (PhS-OCT) system was employed to track the ultrasonically induced SAW impulse. Ex-vivo porcine skin specimen was tested using this proposed method. A 2D fast Fourier transform based algorithm was applied to process the acquired data for estimating the surface acoustic wave dispersion curve and its corresponding penetration depth. Then, the ex-vivo porcine skin elastogram was established by relating the surface acoustic wave dispersion curve and its corresponding penetration depth. The result from the proposed method shows higher lateral resolution than that from current SAW elastography technique, and the approximated skin elastogram could also distinguish the different layers in the skin specimen, i.e. epidermis, dermis and fat layer. This proposed SAW elastography technique may have a large potential to be widely applied in clinical use for skin disease diagnosis and treatment monitoring.

Superresolution parallel magnetic resonance imaging: Application to functional and spectroscopic imaging

PubMed Central

Otazo, Ricardo; Lin, Fa-Hsuan; Wiggins, Graham; Jordan, Ramiro; Sodickson, Daniel; Posse, Stefan

2009-01-01

Standard parallel magnetic resonance imaging (MRI) techniques suffer from residual aliasing artifacts when the coil sensitivities vary within the image voxel. In this work, a parallel MRI approach known as Superresolution SENSE (SURE-SENSE) is presented in which acceleration is performed by acquiring only the central region of k-space instead of increasing the sampling distance over the complete k-space matrix and reconstruction is explicitly based on intra-voxel coil sensitivity variation. In SURE-SENSE, parallel MRI reconstruction is formulated as a superresolution imaging problem where a collection of low resolution images acquired with multiple receiver coils are combined into a single image with higher spatial resolution using coil sensitivities acquired with high spatial resolution. The effective acceleration of conventional gradient encoding is given by the gain in spatial resolution, which is dictated by the degree of variation of the different coil sensitivity profiles within the low resolution image voxel. Since SURE-SENSE is an ill-posed inverse problem, Tikhonov regularization is employed to control noise amplification. Unlike standard SENSE, for which acceleration is constrained to the phase-encoding dimension/s, SURE-SENSE allows acceleration along all encoding directions — for example, two-dimensional acceleration of a 2D echo-planar acquisition. SURE-SENSE is particularly suitable for low spatial resolution imaging modalities such as spectroscopic imaging and functional imaging with high temporal resolution. Application to echo-planar functional and spectroscopic imaging in human brain is presented using two-dimensional acceleration with a 32-channel receiver coil. PMID:19341804

10 CFR 1023.8 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Alternative dispute resolution (ADR). 1023.8 Section 1023..., Functions and Authorities § 1023.8 Alternative dispute resolution (ADR). (a) Statement of Policy. It is the policy of the DOE and of the Board to facilitate consensual resolution of disputes and to employ ADR in...

10 CFR 1023.8 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Alternative dispute resolution (ADR). 1023.8 Section 1023..., Functions and Authorities § 1023.8 Alternative dispute resolution (ADR). (a) Statement of Policy. It is the policy of the DOE and of the Board to facilitate consensual resolution of disputes and to employ ADR in...

Band selective small flip angle COSY: a simple experiment for the analyses of 1H NMR spectra of small chiral molecules.

PubMed

Prabhu, Uday Ramesh; Suryaprakash, N

2008-12-01

The NMR spectroscopic discrimination of enantiomers in the chiral liquid crystalline solvent is more often carried out using (2)H detection in its natural abundance. The employment of (1)H detection for such a purpose is severely hampered due to significant loss of resolution in addition to indistinguishable overlap of the spectra from the two enantiomers. This study demonstrates that the band selected small flip angle homonuclear correlation experiment is a simple and robust technique that provides unambiguous discrimination, very high spectral resolution, reduced multiplicity of transitions, relative signs of the couplings and enormous saving of instrument time.

Flexible Twist for Pitch Control in a High Altitude Long Endurance Aircraft with Nonlinear Response

DTIC Science & Technology

2008-12-01

Information dominance is the key motivator for employing high-altitude long-endurance (HALE) aircraft to provide continuous coverage in the theaters of operation A joined-wing configuration of such a craft gives the advantage of a platform for higher resolution sensors. Design challenges emerge with structural flexibility that arise from a long-endurance aircraft design. The goal was to demonstrate that scaling the nonlinear response of a full-scale finite element model of a high-altitude long-endurance (HALE) aircraft was possible if the model was aeroelastically and

HPC AND GRID COMPUTING FOR INTEGRATIVE BIOMEDICAL RESEARCH

PubMed Central

Kurc, Tahsin; Hastings, Shannon; Kumar, Vijay; Langella, Stephen; Sharma, Ashish; Pan, Tony; Oster, Scott; Ervin, David; Permar, Justin; Narayanan, Sivaramakrishnan; Gil, Yolanda; Deelman, Ewa; Hall, Mary; Saltz, Joel

2010-01-01

Integrative biomedical research projects query, analyze, and integrate many different data types and make use of datasets obtained from measurements or simulations of structure and function at multiple biological scales. With the increasing availability of high-throughput and high-resolution instruments, the integrative biomedical research imposes many challenging requirements on software middleware systems. In this paper, we look at some of these requirements using example research pattern templates. We then discuss how middleware systems, which incorporate Grid and high-performance computing, could be employed to address the requirements. PMID:20107625

A highly versatile automatized setup for quantitative measurements of PHIP enhancements

NASA Astrophysics Data System (ADS)

Kiryutin, Alexey S.; Sauer, Grit; Hadjiali, Sara; Yurkovskaya, Alexandra V.; Breitzke, Hergen; Buntkowsky, Gerd

2017-12-01

The design and application of a versatile and inexpensive experimental extension to NMR spectrometers is described that allows to carry out highly reproducible PHIP experiments directly in the NMR sample tube, i.e. under PASADENA condition, followed by the detection of the NMR spectra of hyperpolarized products with high spectral resolution. Employing this high resolution it is feasible to study kinetic processes in the solution with high accuracy. As a practical example the dissolution of hydrogen gas in the liquid and the PHIP kinetics during the hydrogenation reaction of Fmoc-O-propargyl-L-tyrosine in acetone-d6 are monitored. The timing of the setup is fully controlled by the pulse-programmer of the NMR spectrometer. By flushing with an inert gas it is possible to efficiently quench the hydrogenation reaction in a controlled fashion and to detect the relaxation of hyperpolarization without a background reaction. The proposed design makes it possible to carry out PHIP experiments in an automatic mode and reliably determine the enhancement of polarized signals.

Multiplexed, High Density Electrophysiology with Nanofabricated Neural Probes

PubMed Central

Du, Jiangang; Blanche, Timothy J.; Harrison, Reid R.; Lester, Henry A.; Masmanidis, Sotiris C.

2011-01-01

Extracellular electrode arrays can reveal the neuronal network correlates of behavior with single-cell, single-spike, and sub-millisecond resolution. However, implantable electrodes are inherently invasive, and efforts to scale up the number and density of recording sites must compromise on device size in order to connect the electrodes. Here, we report on silicon-based neural probes employing nanofabricated, high-density electrical leads. Furthermore, we address the challenge of reading out multichannel data with an application-specific integrated circuit (ASIC) performing signal amplification, band-pass filtering, and multiplexing functions. We demonstrate high spatial resolution extracellular measurements with a fully integrated, low noise 64-channel system weighing just 330 mg. The on-chip multiplexers make possible recordings with substantially fewer external wires than the number of input channels. By combining nanofabricated probes with ASICs we have implemented a system for performing large-scale, high-density electrophysiology in small, freely behaving animals that is both minimally invasive and highly scalable. PMID:22022568

Imaging characterization of a new gamma ray detector based on CRY019 scintillation crystal for PET and SPECT applications

NASA Astrophysics Data System (ADS)

Polito, C.; Pani, R.; Trigila, C.; Cinti, M. N.; Fabbri, A.; Frantellizzi, V.; De Vincentis, G.; Pellegrini, R.; Pani, R.

2017-02-01

In the last 40 years, in the field of Molecular Medicine imaging there has been a huge growth in the employment and in the improvement of detectors for PET and SPECT applications in order to reach accurate diagnosis of the diseases. The most important feature required to these detectors is an high quality of images that is usually obtained benefitting from the development of a wide number of new scintillation crystals with high imaging performances. In this contest, features like high detection efficiency, short decay time, great spectral match with photodetectors, absence of afterglow and low costs are surely attractive. However, there are other factors playing an important role in the realization of high quality images such as energy and spatial resolutions, position linearity and contrast resolution. With the aim to realize an high performace gamma ray detector for PET and SPECT applications, this work is focused on the evaluation of the imaging characteristics of a recently developed scintillation crystal, CRY019.

Targeted ENO schemes with tailored resolution property for hyperbolic conservation laws

NASA Astrophysics Data System (ADS)

Fu, Lin; Hu, Xiangyu Y.; Adams, Nikolaus A.

2017-11-01

In this paper, we extend the range of targeted ENO (TENO) schemes (Fu et al. (2016) [18]) by proposing an eighth-order TENO8 scheme. A general formulation to construct the high-order undivided difference τK within the weighting strategy is proposed. With the underlying scale-separation strategy, sixth-order accuracy for τK in the smooth solution regions is designed for good performance and robustness. Furthermore, a unified framework to optimize independently the dispersion and dissipation properties of high-order finite-difference schemes is proposed. The new framework enables tailoring of dispersion and dissipation as function of wavenumber. The optimal linear scheme has minimum dispersion error and a dissipation error that satisfies a dispersion-dissipation relation. Employing the optimal linear scheme, a sixth-order TENO8-opt scheme is constructed. A set of benchmark cases involving strong discontinuities and broadband fluctuations is computed to demonstrate the high-resolution properties of the new schemes.

Development of a near-infrared high-resolution spectrograph (WINERED) for a survey of bulge stars

NASA Astrophysics Data System (ADS)

Tsujimoto, T.; Kobayashi, N.; Yasui, C.; Kondo, S.; Minami, A.; Motohara, K.; Ikeda, Y.; Gouda, N.

2008-07-01

We are developing a new near-infrared high-resolution (R[max] = 100,000) and high-sensitive spectrograph WINERED, which is specifically customized for short NIR bands at 0.9 1.35 μm. WINERED employs an innovative optical system; a portable design and a warm optics without any cold stops. The planned astrometric space mission JASMINE will provide precise positions, distances, and proper motions of the bulge stars. The missing components, the radial velocity and chemical composition will be measured by WINERED. These combined data brought by JASMINE and WINERED will certainly reveal the nature of the Galactic bulge. We plan to complete this instrument for observations of single objects by the end of 2008 and to attach it to various 4 10m telescopes as a PI-type instrument. We hope to upgrade WINERED with a multi-object feed in the future for efficient survey of the JASMINE bulge stars.

High-resolution method for evolving complex interface networks

NASA Astrophysics Data System (ADS)

Pan, Shucheng; Hu, Xiangyu Y.; Adams, Nikolaus A.

2018-04-01

In this paper we describe a high-resolution transport formulation of the regional level-set approach for an improved prediction of the evolution of complex interface networks. The novelty of this method is twofold: (i) construction of local level sets and reconstruction of a global level set, (ii) local transport of the interface network by employing high-order spatial discretization schemes for improved representation of complex topologies. Various numerical test cases of multi-region flow problems, including triple-point advection, single vortex flow, mean curvature flow, normal driven flow, dry foam dynamics and shock-bubble interaction show that the method is accurate and suitable for a wide range of complex interface-network evolutions. Its overall computational cost is comparable to the Semi-Lagrangian regional level-set method while the prediction accuracy is significantly improved. The approach thus offers a viable alternative to previous interface-network level-set method.

X ray imaging microscope for cancer research

NASA Technical Reports Server (NTRS)

Hoover, Richard B.; Shealy, David L.; Brinkley, B. R.; Baker, Phillip C.; Barbee, Troy W., Jr.; Walker, Arthur B. C., Jr.

1991-01-01

The NASA technology employed during the Stanford MSFC LLNL Rocket X Ray Spectroheliograph flight established that doubly reflecting, normal incidence multilayer optics can be designed, fabricated, and used for high resolution x ray imaging of the Sun. Technology developed as part of the MSFC X Ray Microscope program, showed that high quality, high resolution multilayer x ray imaging microscopes are feasible. Using technology developed at Stanford University and at the DOE Lawrence Livermore National Laboratory (LLNL), Troy W. Barbee, Jr. has fabricated multilayer coatings with near theoretical reflectivities and perfect bandpass matching for a new rocket borne solar observatory, the Multi-Spectral Solar Telescope Array (MSSTA). Advanced Flow Polishing has provided multilayer mirror substrates with sub-angstrom (rms) smoothnesss for the astronomical x ray telescopes and x ray microscopes. The combination of these important technological advancements has paved the way for the development of a Water Window Imaging X Ray Microscope for cancer research.

Fiber-Optic Magnetometry and Thermometry Using Optically Detected Magnetic Resonance With Nitrogen-Vacancy Centers in Diamond

NASA Astrophysics Data System (ADS)

Blakley, Sean Michael

Nitrogen--vacancy diamond (NVD) quantum sensors are an emerging technology that has shown great promise in areas like high-resolution thermometry and magnetometry. Optical fibers provide attractive new application paradigms for NVD technology. A detailed description of the fabrication processes associated with the development of novel fiber-optic NVD probes are presented in this work. The demonstrated probes are tested on paradigmatic model systems designed to ascertain their suitability for use in challenging biological environments. Methods employing optically detected magnetic resonance (ODMR) are used to accurately measure and map temperature distributions of small objects and to demonstrate emergent temperature-dependent phenomena in genetically modified living organisms. These methods are also used to create detailed high resolution spatial maps of both magnetic scalar and magnetic vector field distributions of spatially localized weak field features in the presence of a noisy, high-field background.

High-strength bioresorbable Fe-Ag nanocomposite scaffolds: Processing and properties

NASA Astrophysics Data System (ADS)

Sharipova, Aliya; Psakhie, Sergey G.; Swain, Sanjaya K.; Gutmanas, Elazar Y.; Gotman, Irena

2015-10-01

High strength ductile iron-silver nanocomposite scaffolds were fabricated employing high energy attrition milling of micron-submicron powders, followed by cold sintering/high pressure consolidation. Particulate leaching method with soluble Na2SO4 and K2CO3 salts as porogens was used to create scaffolds with 50, 55, 60 and 73% volume fraction of pores. Part of specimens was annealed at 600, 800 and 900°C. Specimens were characterized employing X-ray diffraction, scanning electron microscopy (SEM) with electron probe microanalysis (EDS) and high resolution SEM. Mechanical properties were measured in compression and permeability was measured in permeameter based on Darcy's law. Scaffolds with 50% and 55% porosity exhibited high compressive strength (18-22 MPa), compressive strength of 8-12 MPa was observed for scaffolds with 73% porosity. Treatments at 800 and 900°C result in increase of strength and ductility with some coarsening of microstructure. Best combination of compressive strength (15 MPa) and permeability (0.6-6 cm2) is close to the range of trabecular bone.

Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser

PubMed Central

Han, Kyu Young; Ha, Taekjip

2016-01-01

We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral purity. Selective excitation at different wavelengths permits simultaneous imaging of two fluorescent markers at a nanoscale resolution in three dimensions. PMID:26030581

20 CFR 627.606 - Grant Officer resolution.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Grant Officer resolution. 627.606 Section 627.606 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR GENERAL PROVISIONS... agreement and those where there is disagreement with the recipient's resolution, including the allowability...

Addressing model uncertainty through stochastic parameter perturbations within the High Resolution Rapid Refresh (HRRR) ensemble

NASA Astrophysics Data System (ADS)

Wolff, J.; Jankov, I.; Beck, J.; Carson, L.; Frimel, J.; Harrold, M.; Jiang, H.

2016-12-01

It is well known that global and regional numerical weather prediction ensemble systems are under-dispersive, producing unreliable and overconfident ensemble forecasts. Typical approaches to alleviate this problem include the use of multiple dynamic cores, multiple physics suite configurations, or a combination of the two. While these approaches may produce desirable results, they have practical and theoretical deficiencies and are more difficult and costly to maintain. An active area of research that promotes a more unified and sustainable system for addressing the deficiencies in ensemble modeling is the use of stochastic physics to represent model-related uncertainty. Stochastic approaches include Stochastic Parameter Perturbations (SPP), Stochastic Kinetic Energy Backscatter (SKEB), Stochastic Perturbation of Physics Tendencies (SPPT), or some combination of all three. The focus of this study is to assess the model performance within a convection-permitting ensemble at 3-km grid spacing across the Contiguous United States (CONUS) when using stochastic approaches. For this purpose, the test utilized a single physics suite configuration based on the operational High-Resolution Rapid Refresh (HRRR) model, with ensemble members produced by employing stochastic methods. Parameter perturbations were employed in the Rapid Update Cycle (RUC) land surface model and Mellor-Yamada-Nakanishi-Niino (MYNN) planetary boundary layer scheme. Results will be presented in terms of bias, error, spread, skill, accuracy, reliability, and sharpness using the Model Evaluation Tools (MET) verification package. Due to the high level of complexity of running a frequently updating (hourly), high spatial resolution (3 km), large domain (CONUS) ensemble system, extensive high performance computing (HPC) resources were needed to meet this objective. Supercomputing resources were provided through the National Center for Atmospheric Research (NCAR) Strategic Capability (NSC) project support, allowing for a more extensive set of tests over multiple seasons, consequently leading to more robust results. Through the use of these stochastic innovations and powerful supercomputing at NCAR, further insights and advancements in ensemble forecasting at convection-permitting scales will be possible.

Efficient Probabilistic Forecasting for High-Resolution Models through Clustered-State Data Assimilation

NASA Astrophysics Data System (ADS)

Hamidi, A.; Grossberg, M.; Khanbilvardi, R.

2016-12-01

Flood response in an urban area is the product of interactions of spatially and temporally varying rainfall and infrastructures. In urban areas, however, the complex sub-surface networks of tunnels, waste and storm water drainage systems are often inaccessible, pose challenges for modeling and prediction of the drainage infrastructure performance. The increased availability of open data in cities is an emerging information asset for a better understanding of the dynamics of urban water drainage infrastructure. This includes crowd sourced data and community reporting. A well-known source of this type of data is the non-emergency hotline "311" which is available in many US cities, and may contain information pertaining to the performance of physical facilities, condition of the environment, or residents' experience, comfort and well-being. In this study, seven years of New York City 311 (NYC311) call during 2010-2016 is employed, as an alternative approach for identifying the areas of the city most prone to sewer back up flooding. These zones are compared with the hydrologic analysis of runoff flooding zones to provide a predictive model for the City. The proposed methodology is an example of urban system phenomenology using crowd sourced, open data. A novel algorithm for calculating the spatial distribution of flooding complaints across NYC's five boroughs is presented in this study. In this approach, the features that represent reporting bias are separated from those that relate to actual infrastructure system performance. The sewer backup results are assessed with the spatial distribution of runoff in NYC during 2010-2016. With advances in radar technologies, a high spatial-temporal resolution data set for precipitation is available for most of the United States that can be implemented in hydrologic analysis of dense urban environments. High resolution gridded Stage IV radar rainfall data along with the high resolution spatially distributed land cover data are employed to investigate the urban pluvial flooding. The monthly results of excess runoff are compared with the sewer backup in NYC to build a predictive model of flood zones according to the 311 phone calls.

Two-Photon Polymerization of Defects in Photonic Crystals

DTIC Science & Technology

2006-01-01

technique employs two-photon polymerization (TPP) (for description, see Section 2.2) to fabricate high-resolution 3D embedded polymer features within... polymer , and therefore does not influence the polymerization . The image contrast is from the different reflectivities of the interfaces in the system due...Spectroscopy also confirmed for the first time the successful polymerization of a uniform, dense polymer feature throughout the thickness of the

Recent advances in biotechnological applications of alcohol dehydrogenases.

PubMed

Zheng, Yu-Guo; Yin, Huan-Huan; Yu, Dao-Fu; Chen, Xiang; Tang, Xiao-Ling; Zhang, Xiao-Jian; Xue, Ya-Ping; Wang, Ya-Jun; Liu, Zhi-Qiang

2017-02-01

Alcohol dehydrogenases (ADHs), which belong to the oxidoreductase superfamily, catalyze the interconversion between alcohols and aldehydes or ketones with high stereoselectivity under mild conditions. ADHs are widely employed as biocatalysts for the dynamic kinetic resolution of racemic substrates and for the preparation of enantiomerically pure chemicals. This review provides an overview of biotechnological applications for ADHs in the production of chiral pharmaceuticals and fine chemicals.

The Subaru Coronagraphic Extreme AO project: an XAO4ELT precursor

NASA Astrophysics Data System (ADS)

Martinache, F.

2011-09-01

A diffraction-limited 30-meter telescope theoretically provides a 10 mas resolution limit in the near infrared. Modern coronagraphs like the Vortex, the 8OPM and the PIAA offer the means to take full advantage of this angular resolution allowing to explore at high contrast, the innermost parts of nearby planetary systems to within a fraction of an astronomical unit: an unprecedented capability that will revolutionize our understanding of planet formation across the habitable zone. A precursor of such a system is the Subaru Coronagraphic Extreme AO project. SCExAO combines a high performance PIAA-based coronagraph downstream Subaru's AO188 AO system and a 1024-actuator MEMS DM. SCExAO employs advanced wavefront control schemes that make high contrast detection possible at 1 λ/D, providing for a few cases, the possibility to detect the light reflected by exoplanets. Moderate-high contrast detection in the super-resolution regime (<λ/D) is also possible using well calibrated closure quantities like closure-phase for a non-redundant (masked) aperture and its extension for to arbitrary apertures (Kernel-phase). Lessons learned from SCExAO's incremental deployment plan during its first 2011 engineering campaign provides insights that will guide future development of high contrast instrumentation on an ELT.

Applications of antibodies in microfluidics-based analytical systems: challenges and strategies for success

NASA Astrophysics Data System (ADS)

O’Kennedy, Richard; Fitzgerald, Jenny; Cassedy, Arabelle; Crawley, Aoife; Zhang, Xin; Carrera, Sandro

2018-06-01

This review is designed to focus on antibodies and the attributes that make them ideal for applications in microfluidics-based diagnostic/separation platforms. The structures of different antibody formats and how they can be engineered to be highly effective in microfluidics-based environments will be highlighted. Suggested novel stratagems on the ideal way in which they can be employed in microfluidics systems, based on an informed knowledge of their structures and properties rather than random choice selection, as is often currently employed, will be provided. Finally, a critical assessment of current shortcomings in the approaches used along with possible ways for their resolution will be given.

High resolution telescope including an array of elemental telescopes aligned along a common axis and supported on a space frame with a pivot at its geometric center

DOEpatents

Norbert, M.A.; Yale, O.

1992-04-28

A large effective-aperture, low-cost optical telescope with diffraction-limited resolution enables ground-based observation of near-earth space objects. The telescope has a non-redundant, thinned-aperture array in a center-mount, single-structure space frame. It employes speckle interferometric imaging to achieve diffraction-limited resolution. The signal-to-noise ratio problem is mitigated by moving the wavelength of operation to the near-IR, and the image is sensed by a Silicon CCD. The steerable, single-structure array presents a constant pupil. The center-mount, radar-like mount enables low-earth orbit space objects to be tracked as well as increases stiffness of the space frame. In the preferred embodiment, the array has elemental telescopes with subaperture of 2.1 m in a circle-of-nine configuration. The telescope array has an effective aperture of 12 m which provides a diffraction-limited resolution of 0.02 arc seconds. Pathlength matching of the telescope array is maintained by a electro-optical system employing laser metrology. Speckle imaging relaxes pathlength matching tolerance by one order of magnitude as compared to phased arrays. Many features of the telescope contribute to substantial reduction in costs. These include eliminating the conventional protective dome and reducing on-site construction activities. The cost of the telescope scales with the first power of the aperture rather than its third power as in conventional telescopes. 15 figs.

High resolution telescope including an array of elemental telescopes aligned along a common axis and supported on a space frame with a pivot at its geometric center

DOEpatents

Norbert, Massie A.; Yale, Oster

1992-01-01

A large effective-aperture, low-cost optical telescope with diffraction-limited resolution enables ground-based observation of near-earth space objects. The telescope has a non-redundant, thinned-aperture array in a center-mount, single-structure space frame. It employes speckle interferometric imaging to achieve diffraction-limited resolution. The signal-to-noise ratio problem is mitigated by moving the wavelength of operation to the near-IR, and the image is sensed by a Silicon CCD. The steerable, single-structure array presents a constant pupil. The center-mount, radar-like mount enables low-earth orbit space objects to be tracked as well as increases stiffness of the space frame. In the preferred embodiment, the array has elemental telescopes with subaperture of 2.1 m in a circle-of-nine configuration. The telescope array has an effective aperture of 12 m which provides a diffraction-limited resolution of 0.02 arc seconds. Pathlength matching of the telescope array is maintained by a electro-optical system employing laser metrology. Speckle imaging relaxes pathlength matching tolerance by one order of magnitude as compared to phased arrays. Many features of the telescope contribute to substantial reduction in costs. These include eliminating the conventional protective dome and reducing on-site construction activities. The cost of the telescope scales with the first power of the aperture rather than its third power as in conventional telescopes.

77 FR 13075 - Sunshine Act Meeting Notice

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-05

... resolutions honoring employees for their service, a resolution honoring the 10th anniversary of Radio Sawa, and a resolution for 2012 policy statements on sexual harassment and equal employment opportunity. The.... international broadcasting. The BBG will recognize the anniversaries of Agency language services and receive a...

Cloud-Based Tools to Support High-Resolution Modeling (Invited)

NASA Astrophysics Data System (ADS)

Jones, N.; Nelson, J.; Swain, N.; Christensen, S.

2013-12-01

The majority of watershed models developed to support decision-making by water management agencies are simple, lumped-parameter models. Maturity in research codes and advances in the computational power from multi-core processors on desktop machines, commercial cloud-computing resources, and supercomputers with thousands of cores have created new opportunities for employing more accurate, high-resolution distributed models for routine use in decision support. The barriers for using such models on a more routine basis include massive amounts of spatial data that must be processed for each new scenario and lack of efficient visualization tools. In this presentation we will review a current NSF-funded project called CI-WATER that is intended to overcome many of these roadblocks associated with high-resolution modeling. We are developing a suite of tools that will make it possible to deploy customized web-based apps for running custom scenarios for high-resolution models with minimal effort. These tools are based on a software stack that includes 52 North, MapServer, PostGIS, HT Condor, CKAN, and Python. This open source stack provides a simple scripting environment for quickly configuring new custom applications for running high-resolution models as geoprocessing workflows. The HT Condor component facilitates simple access to local distributed computers or commercial cloud resources when necessary for stochastic simulations. The CKAN framework provides a powerful suite of tools for hosting such workflows in a web-based environment that includes visualization tools and storage of model simulations in a database to archival, querying, and sharing of model results. Prototype applications including land use change, snow melt, and burned area analysis will be presented. This material is based upon work supported by the National Science Foundation under Grant No. 1135482

High-Resolution Graphene Films for Electrochemical Sensing via Inkjet Maskless Lithography.

PubMed

Hondred, John A; Stromberg, Loreen R; Mosher, Curtis L; Claussen, Jonathan C

2017-10-24

Solution-phase printing of nanomaterial-based graphene inks are rapidly gaining interest for fabrication of flexible electronics. However, scalable manufacturing techniques for high-resolution printed graphene circuits are still lacking. Here, we report a patterning technique [i.e., inkjet maskless lithography (IML)] to form high-resolution, flexible, graphene films (line widths down to 20 μm) that significantly exceed the current inkjet printing resolution of graphene (line widths ∼60 μm). IML uses an inkjet printed polymer lacquer as a sacrificial pattern, viscous spin-coated graphene, and a subsequent graphene lift-off to pattern films without the need for prefabricated stencils, templates, or cleanroom technology (e.g., photolithography). Laser annealing is employed to increase conductivity on thermally sensitive, flexible substrates [polyethylene terephthalate (PET)]. Laser annealing and subsequent platinum nanoparticle deposition substantially increases the electroactive nature of graphene as illustrated by electrochemical hydrogen peroxide (H 2 O 2 ) sensing [rapid response (5 s), broad linear sensing range (0.1-550 μm), high sensitivity (0.21 μM/μA), and low detection limit (0.21 μM)]. Moreover, high-resolution, complex graphene circuits [i.e., interdigitated electrodes (IDE) with varying finger width and spacing] were created with IML and characterized via potassium chloride (KCl) electrochemical impedance spectroscopy (EIS). Results indicated that sensitivity directly correlates to electrode feature size as the IDE with the smallest finger width and spacing (50 and 50 μm) displayed the largest response to changes in KCl concentration (∼21 kΩ). These results indicate that the developed IML patterning technique is well-suited for rapid, solution-phase graphene film prototyping on flexible substrates for numerous applications including electrochemical sensing.

Compensation of high order harmonic long quantum-path attosecond chirp

NASA Astrophysics Data System (ADS)

Guichard, R.; Caillat, J.; Lévêque, C.; Risoud, F.; Maquet, A.; Taïeb, R.; Zaïr, A.

2017-12-01

We propose a method to compensate for the extreme ultra violet (XUV) attosecond chirp associated with the long quantum-path in the high harmonic generation process. Our method employs an isolated attosecond pulse (IAP) issued from the short trajectory contribution in a primary target to assist the infrared driving field to produce high harmonics from the long trajectory in a secondary target. In our simulations based on the resolution of the time-dependent Schrödinger equation, the resulting high harmornics present a clear phase compensation of the long quantum-path contribution, near to Fourier transform limited attosecond XUV pulse. Employing time-frequency analysis of the high harmonic dipole, we found that the compensation is not a simple far-field photonic interference between the IAP and the long-path harmonic emission, but a coherent phase transfer from the weak IAP to the long quantum-path electronic wavepacket. Our approach opens the route to utilizing the long quantum-path for the production and applications of attosecond pulses.

Study of cellular oncometabolism via multidimensional protein identification technology.

PubMed

Aukim-Hastie, Claire; Garbis, Spiros D

2014-01-01

Cellular proteomics is becoming a widespread clinical application, matching the definition of bench-to-bedside translation. Among various fields of investigation, this approach can be applied to the study of the metabolic alterations that accompany oncogenesis and tumor progression, which are globally referred to as oncometabolism. Here, we describe a multidimensional protein identification technology (MuDPIT)-based strategy that can be employed to study the cellular proteome of malignant cells and tissues. This method has previously been shown to be compatible with the reproducible, in-depth analysis of up to a thousand proteins in clinical samples. The possibility to employ this technique to study clinical specimens demonstrates its robustness. MuDPIT is advantageous as compared to other approaches because it is direct, highly sensitive, and reproducible, it provides high resolution with ultra-high mass accuracy, it allows for relative quantifications, and it is compatible with multiplexing (thus limiting costs).This method enables the direct assessment of the proteomic profile of neoplastic cells and tissues and could be employed in the near future as a high-throughput, rapid, quantitative, and cost-effective screening platform for clinical samples. © 2014 Elsevier Inc. All rights reserved.

A 5mm catheter for constant resolution probing in Fourier domain optical coherence endoscopy

NASA Astrophysics Data System (ADS)

Lee, Kye-Sung; Wu, Lei; Xie, Huikai; Ilegbusi, Olusegun; Costa, Marco; Rolland, Jannick P.

2007-02-01

A 5mm biophotonic catheter was conceived for optical coherence tomography (OCT) with collimation optics, an axicon lens, and custom design imaging optics, yielding a 360 degree scan aimed at imaging within concave structures such as lung lobes. In OCT a large depth of focus is necessary to image a thick sample with a constant high transverse resolution. There are two approaches to achieving constant lateral resolution in OCT: Dynamic focusing or Bessel beam forming. This paper focuses on imaging with Bessel beams. A Bessel beam can be generated in the sample arm of the OCT interferometer when axicon optics is employed instead of a conventional focusing lens. We present a design for a 5mm catheter that combines an axicon lens with imaging optics and the coupling of a MEMS mirror attached to a micromotor that allow 360 degree scanning with a resolution of about 5 microns across a depth of focus of about 1.2mm.

Pansharpening on the Narrow Vnir and SWIR Spectral Bands of SENTINEL-2

NASA Astrophysics Data System (ADS)

Vaiopoulos, A. D.; Karantzalos, K.

2016-06-01

In this paper results from the evaluation of several state-of-the-art pansharpening techniques are presented for the VNIR and SWIR bands of Sentinel-2. A procedure for the pansharpening is also proposed which aims at respecting the closest spectral similarities between the higher and lower resolution bands. The evaluation included 21 different fusion algorithms and three evaluation frameworks based both on standard quantitative image similarity indexes and qualitative evaluation from remote sensing experts. The overall analysis of the evaluation results indicated that remote sensing experts disagreed with the outcomes and method ranking from the quantitative assessment. The employed image quality similarity indexes and quantitative evaluation framework based on both high and reduced resolution data from the literature didn't manage to highlight/evaluate mainly the spatial information that was injected to the lower resolution images. Regarding the SWIR bands none of the methods managed to deliver significantly better results than a standard bicubic interpolation on the original low resolution bands.

Improved Precision and Accuracy of Quantification of Rare Earth Element Abundances via Medium-Resolution LA-ICP-MS.

PubMed

Funderburg, Rebecca; Arevalo, Ricardo; Locmelis, Marek; Adachi, Tomoko

2017-11-01

Laser ablation ICP-MS enables streamlined, high-sensitivity measurements of rare earth element (REE) abundances in geological materials. However, many REE isotope mass stations are plagued by isobaric interferences, particularly from diatomic oxides and argides. In this study, we compare REE abundances quantitated from mass spectra collected with low-resolution (m/Δm = 300 at 5% peak height) and medium-resolution (m/Δm = 2500) mass discrimination. A wide array of geological samples was analyzed, including USGS and NIST glasses ranging from mafic to felsic in composition, with NIST 610 employed as the bracketing calibrating reference material. The medium-resolution REE analyses are shown to be significantly more accurate and precise (at the 95% confidence level) than low-resolution analyses, particularly in samples characterized by low (<μg/g levels) REE abundances. A list of preferred mass stations that are least susceptible to isobaric interferences is reported. These findings impact the reliability of REE abundances derived from LA-ICP-MS methods, particularly those relying on mass analyzers that do not offer tuneable mass-resolution and/or collision cell technologies that can reduce oxide and/or argide formation. Graphical Abstract ᅟ.

Improved Precision and Accuracy of Quantification of Rare Earth Element Abundances via Medium-Resolution LA-ICP-MS

NASA Astrophysics Data System (ADS)

Funderburg, Rebecca; Arevalo, Ricardo; Locmelis, Marek; Adachi, Tomoko

2017-07-01

Laser ablation ICP-MS enables streamlined, high-sensitivity measurements of rare earth element (REE) abundances in geological materials. However, many REE isotope mass stations are plagued by isobaric interferences, particularly from diatomic oxides and argides. In this study, we compare REE abundances quantitated from mass spectra collected with low-resolution (m/Δm = 300 at 5% peak height) and medium-resolution (m/Δm = 2500) mass discrimination. A wide array of geological samples was analyzed, including USGS and NIST glasses ranging from mafic to felsic in composition, with NIST 610 employed as the bracketing calibrating reference material. The medium-resolution REE analyses are shown to be significantly more accurate and precise (at the 95% confidence level) than low-resolution analyses, particularly in samples characterized by low (<μg/g levels) REE abundances. A list of preferred mass stations that are least susceptible to isobaric interferences is reported. These findings impact the reliability of REE abundances derived from LA-ICP-MS methods, particularly those relying on mass analyzers that do not offer tuneable mass-resolution and/or collision cell technologies that can reduce oxide and/or argide formation.

Analytical strategies for controlling polysorbate-based nanomicelles in fruit juice.

PubMed

Krtkova, Veronika; Schulzova, Vera; Lacina, Ondrej; Hrbek, Vojtech; Tomaniova, Monika; Hajslova, Jana

2014-06-01

This study focused on the detection and quantification of organic micelle-type nanoparticles (NPs) with polysorbate components (polysorbate 20 and polysorbate 80) in their micelle shells that could be used to load biologically active compounds into fruit juice. Several advanced analytical techniques were applied in the stepwise method development strategy used. In the first phase, a system consisting of ultrahigh-performance liquid chromatography employing a size exclusion column coupled with an evaporative light scattering detector (UHPLC-SEC-ELSD) was used for the fractionation of micelle assemblies from other, lower molecular weight sample components. The limit of detection (LoD) of these polysorbate micelles in spiked apple juice was 500 μg mL(-1). After this screening step, mass spectrometric (MS) detection was utilized to confirm the presence of polysorbates in the detected micelles. Two alternative MS techniques were tested: (i) ambient high-resolution mass spectrometry employing a direct analysis in real time ion source coupled with an Orbitrap MS analyzer (DART-Orbitrap MS) enabled fast and simple detection of the polysorbates present in the samples, with a lowest calibration level (LCL) of 1000 μg mL(-1); (ii) ultrahigh-performance reversed-phase liquid chromatography coupled with high-resolution time-of-flight mass spectrometry (UHPLC-HRTOF-MS) provided highly selective and sensitive detection and quantification of polysorbates with an LCL of 0.5 μg mL(-1).

20 CFR 627.805 - Alternative dispute resolution.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Alternative dispute resolution. 627.805 Section 627.805 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR GENERAL... Law Judges § 627.805 Alternative dispute resolution. (a) Parties to a complaint under § 627.801 of...

20 CFR 616.4 - Rules, regulations, procedures, forms-resolution of disagreements.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Rules, regulations, procedures, forms-resolution of disagreements. 616.4 Section 616.4 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION..., procedures, forms—resolution of disagreements. All State agencies shall operate in accordance with such rules...

The Effect of Shadow Area on Sgm Algorithm and Disparity Map Refinement from High Resolution Satellite Stereo Images

NASA Astrophysics Data System (ADS)

Tatar, N.; Saadatseresht, M.; Arefi, H.

2017-09-01

Semi Global Matching (SGM) algorithm is known as a high performance and reliable stereo matching algorithm in photogrammetry community. However, there are some challenges using this algorithm especially for high resolution satellite stereo images over urban areas and images with shadow areas. As it can be seen, unfortunately the SGM algorithm computes highly noisy disparity values for shadow areas around the tall neighborhood buildings due to mismatching in these lower entropy areas. In this paper, a new method is developed to refine the disparity map in shadow areas. The method is based on the integration of potential of panchromatic and multispectral image data to detect shadow areas in object level. In addition, a RANSAC plane fitting and morphological filtering are employed to refine the disparity map. The results on a stereo pair of GeoEye-1 captured over Qom city in Iran, shows a significant increase in the rate of matched pixels compared to standard SGM algorithm.

A near-infrared high-resolution spectroscopic survey of bulge stars - JASMINE prestudy

NASA Astrophysics Data System (ADS)

Tsujimoto, T.; Gouda, N.; Kobayashi, N.; Yasui, C.; Kondo, S.; Minami, A.; Motohara, K.; Ikeda, Y.

2006-08-01

We are developing a new near-infrared high-resolution (R[max]= 100,000) and high-sensitive spectrograph WINERED, which is specifically customized for short NIR bands at 0.9-1.35 μm. WINERED employs the novelty in the optical system; a potable design and a warm optics without any cold stops. The planned astrometric space mission JASMINE will provide the exact positions, distances, and proper motions of the bulge stars. The missing components, the radial velocity and chemical compositions will be measured by WINERED with high accuracies (δV< 1km/s). These combined data brought by JASMINE and WINERED will certainly reveal the nature of the Galactic bulge. We plan to complete this instrument for the observation of a single object by the end of 2008 and hope to attach it to various 4-10m telescopes as a PI-type instrument. In succession, we will develop it to the design for a simultaneous multi-object spectroscopy.

Efficient generation and characterization of spectrally factorable biphotons.

PubMed

Chen, Changchen; Bo, Cao; Niu, Murphy Yuezhen; Xu, Feihu; Zhang, Zheshen; Shapiro, Jeffrey H; Wong, Franco N C

2017-04-03

Spectrally unentangled biphotons with high single-spatiotemporal-mode purity are highly desirable for many quantum information processing tasks. We generate biphotons with an inferred heralded-state spectral purity of 99%, the highest to date without any spectral filtering, by pulsed spontaneous parametric downconversion in a custom-fabricated periodically-poled KTiOPO₄ crystal under extended Gaussian phase-matching conditions. To efficiently characterize the joint spectral intensity of the generated biphotons at high spectral resolution, we employ a commercially available dispersion compensation module (DCM) with a dispersion equivalent to 100 km of standard optical fiber and with an insertion loss of only 2.8 dB. Compared with the typical method of using two temperature-stabilized equal-length fibers that incurs an insertion loss of 20 dB per fiber, the DCM approach achieves high spectral resolution in a much shorter measurement time. Because the dispersion amount and center wavelengths of DCMs can be easily customized, spectral characterization in a wide range of quantum photonic applications should benefit significantly from this technique.

Plasmodium vivax merozoite surface protein-3 alpha: a high-resolution marker for genetic diversity studies.

PubMed

Prajapati, Surendra Kumar; Joshi, Hema; Valecha, Neena

2010-06-01

Malaria, an ancient human infectious disease caused by five species of Plasmodium, among them Plasmodium vivax is the most widespread human malaria species and causes huge morbidity to its host. Identification of genetic marker to resolve higher genetic diversity for an ancient origin organism is a crucial task. We have analyzed genetic diversity of P. vivax field isolates using highly polymorphic antigen gene merozoite surface protein-3 alpha (msp-3 alpha) and assessed its suitability as high-resolution genetic marker for population genetic studies. 27 P. vivax field isolates collected during chloroquine therapeutic efficacy study at Chennai were analyzed for genetic diversity. PCR-RFLP was employed to assess the genetic variations using highly polymorphic antigen gene msp-3 alpha. We observed three distinct PCR alleles at msp-3 alpha, and among them allele A showed significantly high frequency (53%, chi2 = 8.22, p = 0.001). PCR-RFLP analysis revealed 14 and 17 distinct RFLP patterns for Hha1 and Alu1 enzymes respectively. Further, RFLP analysis revealed that allele A at msp-3 alpha is more diverse in the population compared with allele B and C. Combining Hha1 and Alu1 RFLP patterns revealed 21 distinct genotypes among 22 isolates reflects higher diversity resolution power of msp-3 alpha in the field isolates. P. vivax isolates from Chennai region revealed substantial amount of genetic diversity and comparison of allelic diversity with other antigen genes and microsatellites suggesting that msp-3 alpha could be a high-resolution marker for genetic diversity studies among P. vivax field isolates.

Disentangling the effects of novelty, valence and trait anxiety in the bed nucleus of the stria terminalis, amygdala and hippocampus with high resolution 7T fMRI.

PubMed

Pedersen, Walker S; Muftuler, L Tugan; Larson, Christine L

2017-08-01

The hippocampus and amygdala exhibit sensitivity to stimulus novelty that is reduced in participants with inhibited temperament, which is related to trait anxiety. Although the bed nucleus of the stria terminalis (BNST) is highly connected to the amygdala and is implicated in anxiety, whether the BNST responds to novelty remains unstudied, as well as how trait anxiety may modulate this response. Additionally how novelty, stimulus negativity and trait anxiety interact to affect activity in these areas is also unclear. To address these questions, we presented participants with novel and repeated, fearful and neutral faces, while measuring brain activity via fMRI, and also assessed participants' self-reported trait anxiety. As the small size of the BNST makes assessing its activity at typical fMRI resolution difficult, we employed high resolution 7 Tesla scanning. Our results replicate findings of novelty sensitivity that is independent of valence in the hippocampus. Our results also provide novel evidence for a BNST novelty response toward neutral, but not fearful faces. We also found that the novelty response in the hippocampus and BNST was blunted in participants with high trait anxiety. Additionally, we found left amygdala sensitivity to stimulus negativity that was blunted for high trait anxiety participants. These findings extend past research on the response to novel stimuli in the hippocampus and amygdala at high resolution, and are the first to demonstrate trait anxiety modulated novelty sensitivity in the BNST that is dependent on stimulus valence. Copyright © 2017 Elsevier Inc. All rights reserved.

CO tip functionalization in subatomic resolution atomic force microscopy

NASA Astrophysics Data System (ADS)

Kim, Minjung; Chelikowsky, James R.

2015-10-01

Noncontact atomic force microscopy (nc-AFM) employing a CO-functionalized tip displays dramatically enhanced resolution wherein covalent bonds of polycyclic aromatic hydrocarbon can be imaged. Employing real-space pseudopotential first-principles calculations, we examine the role of CO in functionalizing the nc-AFM tip. Our calculations allow us to simulate full AFM images and ascertain the enhancement mechanism of the CO molecule. We consider two approaches: one with an explicit inclusion of the CO molecule and one without. By comparing our simulations to existing experimental images, we ascribe the enhanced resolution of the CO functionalized tip to the special orbital characteristics of the CO molecule.

CO tip functionalization in subatomic resolution atomic force microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Minjung; Chelikowsky, James R.

2015-10-19

Noncontact atomic force microscopy (nc-AFM) employing a CO-functionalized tip displays dramatically enhanced resolution wherein covalent bonds of polycyclic aromatic hydrocarbon can be imaged. Employing real-space pseudopotential first-principles calculations, we examine the role of CO in functionalizing the nc-AFM tip. Our calculations allow us to simulate full AFM images and ascertain the enhancement mechanism of the CO molecule. We consider two approaches: one with an explicit inclusion of the CO molecule and one without. By comparing our simulations to existing experimental images, we ascribe the enhanced resolution of the CO functionalized tip to the special orbital characteristics of the CO molecule.

Automatic Mrf-Based Registration of High Resolution Satellite Video Data

NASA Astrophysics Data System (ADS)

Platias, C.; Vakalopoulou, M.; Karantzalos, K.

2016-06-01

In this paper we propose a deformable registration framework for high resolution satellite video data able to automatically and accurately co-register satellite video frames and/or register them to a reference map/image. The proposed approach performs non-rigid registration, formulates a Markov Random Fields (MRF) model, while efficient linear programming is employed for reaching the lowest potential of the cost function. The developed approach has been applied and validated on satellite video sequences from Skybox Imaging and compared with a rigid, descriptor-based registration method. Regarding the computational performance, both the MRF-based and the descriptor-based methods were quite efficient, with the first one converging in some minutes and the second in some seconds. Regarding the registration accuracy the proposed MRF-based method significantly outperformed the descriptor-based one in all the performing experiments.

High-resolution observations of the QSO 3C 345 at 1.3 centimeters

NASA Technical Reports Server (NTRS)

Baath, L. B.; Ronnang, B. O.; Pauliny-Toth, I. I. K.; Preuss, E.; Witzel, A.; Matveenko, L. I.; Kogan, L. R.; Kostenko, V. I.; Shaffer, D. B.

1981-01-01

High-resolution VLBI observations made at a frequency of 22.235 GHz of the quasar 3C 345 are discussed. Antennas located at the Crimean Astrophysical Observatory, USSR; Onsala, Sweden; Effelsberg, West Germany; and the Haystack Observatory, Massachusetts were employed at 4-min integration times to provide baselines ranging up to 5.5 x 10 to the 8th wavelengths. About 40% of the total flux density of 7.85 Jy, observed in November 1977, and 8.05 Jy, observed in October 1978, is found to originate in an unresolved component of the quasar core in a region less than 0.1 milliarcsec in diameter. The elongated jet-like component of the quasar is observed to contain several peaks of emission extending up to 6 milliarsec from the core which decreased in extent between the two observations.

Applying LED in full-field optical coherence tomography for gastrointestinal endoscopy

NASA Astrophysics Data System (ADS)

Yang, Bor-Wen; Wang, Yu-Yen; Juan, Yu-Shan; Hsu, Sheng-Jie

2015-08-01

Optical coherence tomography (OCT) has become an important medical imaging technology due to its non-invasiveness and high resolution. Full-field optical coherence tomography (FF-OCT) is a scanning scheme especially suitable for en face imaging as it employs a CMOS/CCD device for parallel pixels processing. FF-OCT can also be applied to high-speed endoscopic imaging. Applying cylindrical scanning and a right-angle prism, we successfully obtained a 360° tomography of the inner wall of an intestinal cavity through an FF-OCT system with an LED source. The 10-μm scale resolution enables the early detection of gastrointestinal lesions, which can increase detection rates for esophageal, stomach, or vaginal cancer. All devices used in this system can be integrated by MOEMS technology to contribute to the studies of gastrointestinal medicine and advanced endoscopy technology.

Superior spatial resolution in confocal X-ray techniques using collimating channel array optics: elemental mapping and speciation in archaeological human bone

DOE Office of Scientific and Technical Information (OSTI.GOV)

Choudhury, S.; Agyeman-Budu, D. N.; Woll, A. R.

Confocal X-ray fluorescence imaging (CXFI) and confocal X-ray absorption spectroscopy (CXAS) respectively enable the study of three dimensionally resolved localization and speciation of elements. Applied to a thick sample, essentially any volume element of interest within the X-ray fluorescence escape depth can be examined without the need for physical thin sectioning. To date, X-ray confocal detection generally has employed a polycapillary optic in front of the detector to collect fluorescence from the probe volume formed at the intersection of its focus with the incident microfocus beam. This work demonstrates the capability of a novel Collimating Channel Array (CCA) optic inmore » providing an improved and essentially energy independent depth resolution approaching 2 μm. By presenting a comparison of elemental maps of archaeological bone collected without confocal detection, and with polycapillary- and CCA-based confocal detection, this study highlights the strengths and limitations of each mode. Unlike the polycapillary, the CCA shows similar spatial resolution in maps for both low (Ca) and high (Pb and Sr) energy X-ray fluorescence, thus illustrating the energy independent nature of the CCA optic resolution. While superior spatial resolution is demonstrated for all of these elements, the most significant improvement is observed for Ca, demonstrating the advantage of employing the CCA optic in examining light elements. In addition to CXFI, this configuration also enables the collection of Pb L3 CXAS data from micro-volumes with dimensions comparable to bone microstructures of interest. Our CXAS result, which represents the first CCA-based biological CXAS, demonstrates the ability of CCA optics to collect site specific spectroscopic information. The demonstrated combination of site-specific elemental localization and speciation data will be useful in diverse fields.« less

SOMAR-LES: A framework for multi-scale modeling of turbulent stratified oceanic flows

NASA Astrophysics Data System (ADS)

Chalamalla, Vamsi K.; Santilli, Edward; Scotti, Alberto; Jalali, Masoud; Sarkar, Sutanu

2017-12-01

A new multi-scale modeling technique, SOMAR-LES, is presented in this paper. Localized grid refinement gives SOMAR (the Stratified Ocean Model with Adaptive Resolution) access to small scales of the flow which are normally inaccessible to general circulation models (GCMs). SOMAR-LES drives a LES (Large Eddy Simulation) on SOMAR's finest grids, forced with large scale forcing from the coarser grids. Three-dimensional simulations of internal tide generation, propagation and scattering are performed to demonstrate this multi-scale modeling technique. In the case of internal tide generation at a two-dimensional bathymetry, SOMAR-LES is able to balance the baroclinic energy budget and accurately model turbulence losses at only 10% of the computational cost required by a non-adaptive solver running at SOMAR-LES's fine grid resolution. This relative cost is significantly reduced in situations with intermittent turbulence or where the location of the turbulence is not known a priori because SOMAR-LES does not require persistent, global, high resolution. To illustrate this point, we consider a three-dimensional bathymetry with grids adaptively refined along the tidally generated internal waves to capture remote mixing in regions of wave focusing. The computational cost in this case is found to be nearly 25 times smaller than that of a non-adaptive solver at comparable resolution. In the final test case, we consider the scattering of a mode-1 internal wave at an isolated two-dimensional and three-dimensional topography, and we compare the results with Legg (2014) numerical experiments. We find good agreement with theoretical estimates. SOMAR-LES is less dissipative than the closure scheme employed by Legg (2014) near the bathymetry. Depending on the flow configuration and resolution employed, a reduction of more than an order of magnitude in computational costs is expected, relative to traditional existing solvers.

Root causes investigation of catastrophic optical bulk damage in high-power InGaAs-AlGaAs strained QW lasers

NASA Astrophysics Data System (ADS)

Sin, Yongkun; Lingley, Zachary; Ayvazian, Talin; Brodie, Miles; Ives, Neil

2018-02-01

High-power single-mode (SM) and multi-mode (MM) InGaAs-AlGaAs strained quantum well (QW) lasers are critical components for both terrestrial and space satellite communications systems. Since these lasers predominantly fail by catastrophic and sudden degradation due to COD, it is especially crucial for space satellite applications to investigate reliability, failure modes, and degradation mechanisms of these lasers. Our group reported a new failure mode in MM and SM InGaAs-AlGaAs strained QW lasers in 2009 and 2016, respectively. Our group also reported in 2017 that bulk failure due to catastrophic optical bulk damage (COBD) is the dominant failure mode of both SM and MM lasers that were subject to long-term life-tests. For the present study, we report root causes investigation of COBD by performing long-term lifetests followed by failure mode analysis (FMA) using various micro-analytical techniques including electron beam induced current (EBIC), time-resolved electroluminescence (EL), focused ion beam (FIB), high-resolution transmission electron microscopy (TEM), and deep level transient spectroscopy (DLTS). Our life-tests with accumulated test hours of over 25,000 hours for SM lasers and over 35,000 hours for MM lasers generated a number of COBD failures with various failure times. EBIC techniques were employed to study dark line defects (DLDs) generated in SM COBD failures stressed under different test conditions. FIB and high-resolution TEM were employed to prepare cross sectional and plan view TEM specimens to study DLD areas (dislocations) in post-aged SM lasers. Time-resolved EL techniques were employed to study initiation and progressions of dark spots and dark lines in real time as MM lasers were aged. Lastly, to investigate precursor signatures of failure and degradation mechanisms responsible for COBD in both SM and MM lasers, we employed DLTS techniques to study a role that electron traps (non-radiative recombination centers) play in degradation of these lasers. Our in-depth root causes investigation results are reported.

MRI uncovers disrupted hippocampal microstructure that underlies memory impairments after early-life adversity.

PubMed

Molet, Jenny; Maras, Pamela M; Kinney-Lang, Eli; Harris, Neil G; Rashid, Faisal; Ivy, Autumn S; Solodkin, Ana; Obenaus, Andre; Baram, Tallie Z

2016-12-01

Memory and related cognitive functions are progressively impaired in a subgroup of individuals experiencing childhood adversity and stress. However, it is not possible to identify vulnerable individuals early, a crucial step for intervention. In this study, high-resolution magnetic resonance imaging (MRI) and intra-hippocampal diffusion tensor imaging (DTI) were employed to examine for structural signatures of cognitive adolescent vulnerabilities in a rodent model of early-life adversity. These methods were complemented by neuroanatomical and functional assessments of hippocampal network integrity during adolescence, adulthood and middle-age. The high-resolution MRI identified selective loss of dorsal hippocampal volume, and intra-hippocampal DTI uncovered disruption of dendritic structure, consistent with disrupted local connectivity, already during late adolescence in adversity-experiencing rats. Memory deteriorated over time, and stunting of hippocampal dendritic trees was apparent on neuroanatomical analyses. Thus, disrupted hippocampal neuronal structure and connectivity, associated with cognitive impairments, are detectable via non-invasive imaging modalities in rats experiencing early-life adversity. These high-resolution imaging approaches may constitute promising tools for prediction and assessment of at-risk individuals in the clinic. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Semantic labeling of high-resolution aerial images using an ensemble of fully convolutional networks

NASA Astrophysics Data System (ADS)

Sun, Xiaofeng; Shen, Shuhan; Lin, Xiangguo; Hu, Zhanyi

2017-10-01

High-resolution remote sensing data classification has been a challenging and promising research topic in the community of remote sensing. In recent years, with the rapid advances of deep learning, remarkable progress has been made in this field, which facilitates a transition from hand-crafted features designing to an automatic end-to-end learning. A deep fully convolutional networks (FCNs) based ensemble learning method is proposed to label the high-resolution aerial images. To fully tap the potentials of FCNs, both the Visual Geometry Group network and a deeper residual network, ResNet, are employed. Furthermore, to enlarge training samples with diversity and gain better generalization, in addition to the commonly used data augmentation methods (e.g., rotation, multiscale, and aspect ratio) in the literature, aerial images from other datasets are also collected for cross-scene learning. Finally, we combine these learned models to form an effective FCN ensemble and refine the results using a fully connected conditional random field graph model. Experiments on the ISPRS 2-D Semantic Labeling Contest dataset show that our proposed end-to-end classification method achieves an overall accuracy of 90.7%, a state-of-the-art in the field.

Registration performance on EUV masks using high-resolution registration metrology

NASA Astrophysics Data System (ADS)

Steinert, Steffen; Solowan, Hans-Michael; Park, Jinback; Han, Hakseung; Beyer, Dirk; Scherübl, Thomas

2016-10-01

Next-generation lithography based on EUV continues to move forward to high-volume manufacturing. Given the technical challenges and the throughput concerns a hybrid approach with 193 nm immersion lithography is expected, at least in the initial state. Due to the increasing complexity at smaller nodes a multitude of different masks, both DUV (193 nm) and EUV (13.5 nm) reticles, will then be required in the lithography process-flow. The individual registration of each mask and the resulting overlay error are of crucial importance in order to ensure proper functionality of the chips. While registration and overlay metrology on DUV masks has been the standard for decades, this has yet to be demonstrated on EUV masks. Past generations of mask registration tools were not necessarily limited in their tool stability, but in their resolution capabilities. The scope of this work is an image placement investigation of high-end EUV masks together with a registration and resolution performance qualification. For this we employ a new generation registration metrology system embedded in a production environment for full-spec EUV masks. This paper presents excellent registration performance not only on standard overlay markers but also on more sophisticated e-beam calibration patterns.

Synthetic aperture ultrasound imaging with a ring transducer array: preliminary ex vivo results.

PubMed

Qu, Xiaolei; Azuma, Takashi; Yogi, Takeshi; Azuma, Shiho; Takeuchi, Hideki; Tamano, Satoshi; Takagi, Shu

2016-10-01

The conventional medical ultrasound imaging has a low lateral spatial resolution, and the image quality depends on the depth of the imaging location. To overcome these problems, this study presents a synthetic aperture (SA) ultrasound imaging method using a ring transducer array. An experimental ring transducer array imaging system was constructed. The array was composed of 2048 transducer elements, and had a diameter of 200 mm and an inter-element pitch of 0.325 mm. The imaging object was placed in the center of the ring transducer array, which was immersed in water. SA ultrasound imaging was then employed to scan the object and reconstruct the reflection image. Both wire phantom and ex vivo experiments were conducted. The proposed method was found to be capable of producing isotropic high-resolution images of the wire phantom. In addition, preliminary ex vivo experiments using porcine organs demonstrated the ability of the method to reconstruct high-quality images without any depth dependence. The proposed ring transducer array and SA ultrasound imaging method were shown to be capable of producing isotropic high-resolution images whose quality was independent of depth.

20 CFR 667.510 - What is the Grant Officer resolution process?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false What is the Grant Officer resolution process? 667.510 Section 667.510 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF... From Monitoring and Oversight Reviews § 667.510 What is the Grant Officer resolution process? (a...

20 CFR 667.510 - What is the Grant Officer resolution process?

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 4 2014-04-01 2014-04-01 false What is the Grant Officer resolution process? 667.510 Section 667.510 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF... Findings From Monitoring and Oversight Reviews § 667.510 What is the Grant Officer resolution process? (a...

Photo-Induced Click Chemistry for DNA Surface Structuring by Direct Laser Writing.

PubMed

Kerbs, Antonina; Mueller, Patrick; Kaupp, Michael; Ahmed, Ishtiaq; Quick, Alexander S; Abt, Doris; Wegener, Martin; Niemeyer, Christof M; Barner-Kowollik, Christopher; Fruk, Ljiljana

2017-04-11

Oligonucleotides containing photo-caged dienes were prepared and shown to react quantitatively in a light-induced Diels-Alder cycloaddition with functional maleimides in aqueous solution within minutes. Due to its high yield and fast rate, the reaction was exploited for DNA surface patterning with sub-micrometer resolution employing direct laser writing (DLW). Functional DNA arrays were written by direct laser writing (DLW) in variable patterns, which were further encoded with fluorophores and proteins through DNA directed immobilization. This mild and efficient light-driven platform technology holds promise for the fabrication of complex bioarrays with sub-micron resolution. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Theory and design of interferometric synthetic aperture radars

NASA Technical Reports Server (NTRS)

Rodriguez, E.; Martin, J. M.

1992-01-01

A derivation of the signal statistics, an optimal estimator of the interferometric phase, and the expression necessary to calculate the height-error budget are presented. These expressions are used to derive methods of optimizing the parameters of the interferometric synthetic aperture radar system (InSAR), and are then employed in a specific design example for a system to perform high-resolution global topographic mapping with a one-year mission lifetime, subject to current technological constraints. A Monte Carlo simulation of this InSAR system is performed to evaluate its performance for realistic topography. The results indicate that this system has the potential to satisfy the stringent accuracy and resolution requirements for geophysical use of global topographic data.

Modified Fabry-Perot interferometer for displacement measurement in ultra large measuring range

NASA Astrophysics Data System (ADS)

Chang, Chung-Ping; Tung, Pi-Cheng; Shyu, Lih-Horng; Wang, Yung-Cheng; Manske, Eberhard

2013-05-01

Laser interferometers have demonstrated outstanding measuring performances for high precision positioning or dimensional measurements in the precision industry, especially in the length measurement. Due to the non-common-optical-path structure, appreciable measurement errors can be easily induced under ordinary measurement conditions. That will lead to the limitation and inconvenience for in situ industrial applications. To minimize the environmental and mechanical effects, a new interferometric displacement measuring system with the common-optical-path structure and the resistance to tilt-angle is proposed. With the integration of optomechatronic modules in the novel interferometric system, the resolution up to picometer order, high precision, and ultra large measuring range have been realized. For the signal stabilization of displacement measurement, an automatic gain control module has been proposed. A self-developed interpolation model has been employed for enhancing the resolution. The novel interferometer can hold the advantage of high resolution and large measuring range simultaneously. By the experimental verifications, it has been proven that the actual resolution of 2.5 nm can be achieved in the measuring range of 500 mm. According to the comparison experiments, the maximal standard deviation of the difference between the self-developed Fabry-Perot interferometer and the reference commercial Michelson interferometer is 0.146 μm in the traveling range of 500 mm. With the prominent measuring characteristics, this should be the largest dynamic measurement range of a Fabry-Perot interferometer up till now.

A high-resolution 3D ultrasonic system for rapid evaluation of the anterior and posterior segment.

PubMed

Peyman, Gholam A; Ingram, Charles P; Montilla, Leonardo G; Witte, Russell S

2012-01-01

Traditional ultrasound imaging systems for ophthalmology employ slow, mechanical scanning of a single-element ultrasound transducer. The goal was to demonstrate rapid examination of the anterior and posterior segment with a three-dimensional (3D) commercial ultrasound system incorporating high-resolution linear probe arrays. The 3D images of the porcine eye were generated in approximately 10 seconds by scanning one of two commercial linear arrays (25- and 50-MHz). Healthy enucleated pig eyes were compared with those with induced injury or placement of a foreign material (eg, metal). Rapid, volumetric imaging was also demonstrated in one human eye in vivo. The 50-MHz probe provided exquisite volumetric images of the anterior segment at a depth up to 15 mm and axial resolution of 30 μm. The 25-MHz probe provided a larger field of view (lateral X depth: 20 × 30 mm), sufficient for capturing the entire anterior and posterior segments of the pig eye, at a resolution of 60 μm. A 50-MHz scan through the human eyelid illustrated detailed structures of the Meibomian glands, cilia, cornea, and anterior segment back to the posterior capsule. The 3D system with its high-frequency ultrasound arrays, fast data acquisition, and volume rendering capability shows promise for investigating anterior and posterior structures of the eye. Copyright 2012, SLACK Incorporated.

Multi-GPU Accelerated Admittance Method for High-Resolution Human Exposure Evaluation.

PubMed

Xiong, Zubiao; Feng, Shi; Kautz, Richard; Chandra, Sandeep; Altunyurt, Nevin; Chen, Ji

2015-12-01

A multi-graphics processing unit (GPU) accelerated admittance method solver is presented for solving the induced electric field in high-resolution anatomical models of human body when exposed to external low-frequency magnetic fields. In the solver, the anatomical model is discretized as a three-dimensional network of admittances. The conjugate orthogonal conjugate gradient (COCG) iterative algorithm is employed to take advantage of the symmetric property of the complex-valued linear system of equations. Compared against the widely used biconjugate gradient stabilized method, the COCG algorithm can reduce the solving time by 3.5 times and reduce the storage requirement by about 40%. The iterative algorithm is then accelerated further by using multiple NVIDIA GPUs. The computations and data transfers between GPUs are overlapped in time by using asynchronous concurrent execution design. The communication overhead is well hidden so that the acceleration is nearly linear with the number of GPU cards. Numerical examples show that our GPU implementation running on four NVIDIA Tesla K20c cards can reach 90 times faster than the CPU implementation running on eight CPU cores (two Intel Xeon E5-2603 processors). The implemented solver is able to solve large dimensional problems efficiently. A whole adult body discretized in 1-mm resolution can be solved in just several minutes. The high efficiency achieved makes it practical to investigate human exposure involving a large number of cases with a high resolution that meets the requirements of international dosimetry guidelines.

Electron precipitation response to geomagnetic pulsations: Riometer revelation

NASA Astrophysics Data System (ADS)

Honary, Farideh; Kavanagh, Andrew

Electron precipitation modulations by geomagnetic pulsation have been observed in cosmic noise absorption (CNA) as early as 1965 by widebeam riometers (Barcus and Rosenberg, 1965). The first observation of pulsation with high m-number was reported by Kikuchi et al.(1988) em-ploying a scanning narrow-beam riometer to investigate the spatial structure in one dimension with a high resolution. However, the advances in high spatial resolution imaging riometers has provided the ability to observe pulsating cosmic noise absorption with azimuthal wave numbers as high as 380 as well as providing the capability of mapping their structures. These waves are commonly observed during the morning and early afternoon and exhibit eastward propagation. In this presentation a complete generating mechanism for these high m-number waves is dis-cussed as a five step process, beginning with the solar wind as a source for the excitation of dayside magnetospheric cavity modes, mode conversion, energisation of drift-bounce protons by Landau damping, followed by inverse Landau damping as a driving mechanism for the high m number secondary waves that ultimately modulate the electron precipitation. This modulation is observed as pulsations in cosmic noise absorption.

Experimental flat-panel high-spatial-resolution volume CT of the temporal bone.

PubMed

Gupta, Rajiv; Bartling, Soenke H; Basu, Samit K; Ross, William R; Becker, Hartmut; Pfoh, Armin; Brady, Thomas; Curtin, Hugh D

2004-09-01

A CT scanner employing a digital flat-panel detector is capable of very high spatial resolution as compared with a multi-section CT (MSCT) scanner. Our purpose was to determine how well a prototypical volume CT (VCT) scanner with a flat-panel detector system defines fine structures in temporal bone. Four partially manipulated temporal-bone specimens were imaged by use of a prototypical cone-beam VCT scanner with a flat-panel detector system at an isometric resolution of 150 microm at the isocenter. These specimens were also depicted by state-of-the-art multisection CT (MSCT). Forty-two structures imaged by both scanners were qualitatively assessed and rated, and scores assigned to VCT findings were compared with those of MSCT. Qualitative assessment of anatomic structures, lesions, cochlear implants, and middle-ear hearing aids indicated that image quality was significantly better with VCT (P < .001). Structures near the spatial-resolution limit of MSCT (e.g., bony covering of the tympanic segment of the facial canal, the incudo-stapedial joint, the proximal vestibular aqueduct, the interscalar septum, and the modiolus) had higher contrast and less partial-volume effect with VCT. The flat-panel prototype provides better definition of fine osseous structures of temporal bone than that of currently available MSCT scanners. This study provides impetus for further research in increasing spatial resolution beyond that offered by the current state-of-the-art scanners.

SWICS/Ulysses and MASS/wind observations of solar wind sulfur charge states

NASA Technical Reports Server (NTRS)

Cohen, C. M. S.; Galvin, A. B.; Hamilton, D. C.; Gloeckler, G.; Geiss, J.; Bochsler, P.

1995-01-01

As Ulysses journeys from the southern to the northern solar pole, the newly launched Wind spacecraft is monitoring the solar wind near 1 AU, slightly upstream of the Earth. Different solar wind structures pass over both spacecraft as coronal holes and other features rotate in and out of view. Ulysses and Wind are presently on opposing sides of the sun allowing us to monitor these streams for extended periods of time. Composition measurements made by instruments on both spacecraft provide information concerning the evolution and properties of these structures. We have combined data from the Solar Wind Ion Composition Spectrometer (SWICS) on Ulysses and the high mass resolution spectrometer (MASS) on Wind to determine the charge state distribution of sulfur in the solar wind. Both instruments employ electrostatic deflection with time-of-flight measurement. The high mass resolution of the MASS instrument (M/Delta-M approximately 100) allows sulfur to be isolated easily while the stepping energy/charge selection provides charge state information. SWICS measurements allow the unique identification of heavy ions by their mass and mass/charge with resolutions of M/Delta-M approximately 3 and M/q/Delta(M/q) approximately 20. The two instruments complement each other nicely in that MASS has the greater mass resolution while SWICS has the better mass/charge resolution and better statistics.

A compact high resolution ion mobility spectrometer for fast trace gas analysis.

PubMed

Kirk, Ansgar T; Allers, Maria; Cochems, Philipp; Langejuergen, Jens; Zimmermann, Stefan

2013-09-21

Drift tube ion mobility spectrometers (IMS) are widely used for fast trace gas detection in air, but portable compact systems are typically very limited in their resolving power. Decreasing the initial ion packet width improves the resolution, but is generally associated with a reduced signal-to-noise-ratio (SNR) due to the lower number of ions injected into the drift region. In this paper, we present a refined theory of IMS operation which employs a combined approach for the analysis of the ion drift and the subsequent amplification to predict both the resolution and the SNR of the measured ion current peak. This theoretical analysis shows that the SNR is not a function of the initial ion packet width, meaning that compact drift tube IMS with both very high resolution and extremely low limits of detection can be designed. Based on these implications, an optimized combination of a compact drift tube with a length of just 10 cm and a transimpedance amplifier has been constructed with a resolution of 183 measured for the positive reactant ion peak (RIP(+)), which is sufficient to e.g. separate the RIP(+) from the protonated acetone monomer, even though their drift times only differ by a factor of 1.007. Furthermore, the limits of detection (LODs) for acetone are 180 pptv within 1 s of averaging time and 580 pptv within only 100 ms.

Analysis of the precipitation and streamflow extremes in Northern Italy using high resolution reanalysis dataset Express-Hydro

NASA Astrophysics Data System (ADS)

Silvestro, Francesco; Parodi, Antonio; Campo, Lorenzo

2017-04-01

The characterization of the hydrometeorological extremes, both in terms of rainfall and streamflow, in a given region plays a key role in the environmental monitoring provided by the flood alert services. In last years meteorological simulations (both near real-time and historical reanalysis) were available at increasing spatial and temporal resolutions, making possible long-period hydrological reanalysis in which the meteo dataset is used as input in distributed hydrological models. In this work, a very high resolution meteorological reanalysis dataset, namely Express-Hydro (CIMA, ISAC-CNR, GAUSS Special Project PR45DE), was employed as input in the hydrological model Continuum in order to produce long time series of streamflows in the Liguria territory, located in the Northern part of Italy. The original dataset covers the whole Europe territory in the 1979-2008 period, at 4 km of spatial resolution and 3 hours of time resolution. Analyses in terms of comparison between the rainfall estimated by the dataset and the observations (available from the local raingauges network) were carried out, and a bias correction was also performed in order to better match the observed climatology. An extreme analysis was eventually carried on the streamflows time series obtained by the simulations, by comparing them with the results of the same hydrological model fed with the observed time series of rainfall. The results of the analysis are shown and discussed.

Middle atmosphere simulated with high vertical and horizontal resolution versions of a GCM: Improvements in the cold pole bias and generation of a QBO-like oscillation in the tropics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hamilton, K.; Wilson, R.J.; Hemler, R.S.

1999-11-15

The large-scale circulation in the Geophysical Fluid Dynamics Laboratory SKYHI troposphere-stratosphere-mesosphere finite-difference general circulation model is examined as a function of vertical and horizontal resolution. The experiments examined include one with horizontal grid spacing of {approximately}35 km and another with {approximately}100 km horizontal grid spacing but very high vertical resolution (160 levels between the ground and about 85 km). The simulation of the middle-atmospheric zonal-mean winds and temperatures in the extratropics is found to be very sensitive to horizontal resolution. For example, in the early Southern Hemisphere winter the South Pole near 1 mb in the model is colder thanmore » observed, but the bias is reduced with improved horizontal resolution (from {approximately}70 C in a version with {approximately}300 km grid spacing to less than 10 C in the {approximately}35 km version). The extratropical simulation is found to be only slightly affected by enhancements of the vertical resolution. By contrast, the tropical middle-atmospheric simulation is extremely dependent on the vertical resolution employed. With level spacing in the lower stratosphere {approximately}1.5 km, the lower stratospheric zonal-mean zonal winds in the equatorial region are nearly constant in time. When the vertical resolution is doubled, the simulated stratospheric zonal winds exhibit a strong equatorially centered oscillation with downward propagation of the wind reversals and with formation of strong vertical shear layers. This appears to be a spontaneous internally generated oscillation and closely resembles the observed QBO in many respects, although the simulated oscillation has a period less than half that of the real QBO.« less

SP_Ace: Stellar Parameters And Chemical abundances Estimator

NASA Astrophysics Data System (ADS)

Boeche, C.; Grebel, E. K.

2018-05-01

SP_Ace (Stellar Parameters And Chemical abundances Estimator) estimates the stellar parameters Teff, log g, [M/H], and elemental abundances. It employs 1D stellar atmosphere models in Local Thermodynamic Equilibrium (LTE). The code is highly automated and suitable for analyzing the spectra of large spectroscopic surveys with low or medium spectral resolution (R = 2000-20 000). A web service for calculating these values with the software is also available.

Tutorial on photoacoustic tomography

NASA Astrophysics Data System (ADS)

Zhou, Yong; Yao, Junjie; Wang, Lihong V.

2016-06-01

Photoacoustic tomography (PAT) has become one of the fastest growing fields in biomedical optics. Unlike pure optical imaging, such as confocal microscopy and two-photon microscopy, PAT employs acoustic detection to image optical absorption contrast with high-resolution deep into scattering tissue. So far, PAT has been widely used for multiscale anatomical, functional, and molecular imaging of biological tissues. We focus on PAT's basic principles, major implementations, imaging contrasts, and recent applications.

Forest fuel treatment detection using multi-temporal airborne lidar data and high-resolution aerial imagery: a case study in the Sierra Nevada Mountains, California

Treesearch

Yanjun Su; Qinghua Guo; Brandon M. Collins; Danny L. Fry; Tianyu Hu; Maggi Kelly

2016-01-01

Treatments to reduce forest fuels are often performed in forests to enhance forest health, regulate stand density, and reduce the risk of wildfires. Although commonly employed, there are concerns that these forest fuel treatments (FTs) may have negative impacts on certain wildlife species. Often FTs are planned across large landscapes, but the actual treatment extents...

Temporal variation and scale in movement-based resource selection functions

USGS Publications Warehouse

Hooten, M.B.; Hanks, E.M.; Johnson, D.S.; Alldredge, M.W.

2013-01-01

A common population characteristic of interest in animal ecology studies pertains to the selection of resources. That is, given the resources available to animals, what do they ultimately choose to use? A variety of statistical approaches have been employed to examine this question and each has advantages and disadvantages with respect to the form of available data and the properties of estimators given model assumptions. A wealth of high resolution telemetry data are now being collected to study animal population movement and space use and these data present both challenges and opportunities for statistical inference. We summarize traditional methods for resource selection and then describe several extensions to deal with measurement uncertainty and an explicit movement process that exists in studies involving high-resolution telemetry data. Our approach uses a correlated random walk movement model to obtain temporally varying use and availability distributions that are employed in a weighted distribution context to estimate selection coefficients. The temporally varying coefficients are then weighted by their contribution to selection and combined to provide inference at the population level. The result is an intuitive and accessible statistical procedure that uses readily available software and is computationally feasible for large datasets. These methods are demonstrated using data collected as part of a large-scale mountain lion monitoring study in Colorado, USA.

Ultrafast High-Resolution Mass Spectrometric Finger Pore Imaging in Latent Finger Prints

NASA Astrophysics Data System (ADS)

Elsner, Christian; Abel, Bernd

2014-11-01

Latent finger prints (LFPs) are deposits of sweat components in ridge and groove patterns, left after human fingers contact with a surface. Being important targets in biometry and forensic investigations they contain more information than topological patterns. With laser desorption mass spectrometry imaging (LD-MSI) we record `three-dimensional' finger prints with additional chemical information as the third dimension. Here we show the potential of fast finger pore imaging (FPI) in latent finger prints employing LD-MSI without a classical matrix in a high- spatial resolution mode. Thin films of gold rapidly sputtered on top of the sample are used for desorption. FPI employing an optical image for rapid spatial orientation and guiding of the desorption laser enables the rapid analysis of individual finger pores, and the chemical composition of their excretions. With this approach we rapidly detect metabolites, drugs, and characteristic excretions from the inside of the human organism by a minimally-invasive strategy, and distinguish them from chemicals in contact with fingers without any labeling. The fast finger pore imaging, analysis, and screening approach opens the door for a vast number of novel applications in such different fields as forensics, doping and medication control, therapy, as well as rapid profiling of individuals.

Miniature all-optical probe for photoacoustic and ultrasound dual-modality imaging

NASA Astrophysics Data System (ADS)

Li, Guangyao; Guo, Zhendong; Chen, Sung-Liang

2018-02-01

Photoacoustic (PA) imaging forms an image based on optical absorption contrasts with ultrasound (US) resolution. In contrast, US imaging is based on acoustic backscattering to provide structural information. In this study, we develop a miniature all-optical probe for high-resolution PA-US dual-modality imaging over a large imaging depth range. The probe employs three individual optical fibers (F1-F3) to achieve optical generation and detection of acoustic waves for both PA and US modalities. To offer wide-angle laser illumination, fiber F1 with a large numerical aperture (NA) is used for PA excitation. On the other hand, wide-angle US waves are generated by laser illumination on an optically absorbing composite film which is coated on the end face of fiber F2. Both the excited PA and backscattered US waves are detected by a Fabry-Pérot cavity on the tip of fiber F3 for wide-angle acoustic detection. The wide angular features of the three optical fibers make large-NA synthetic aperture focusing technique possible and thus high-resolution PA and US imaging. The probe diameter is less than 2 mm. Over a depth range of 4 mm, lateral resolutions of PA and US imaging are 104-154 μm and 64-112 μm, respectively, and axial resolutions of PA and US imaging are 72-117 μm and 31-67 μm, respectively. To show the imaging capability of the probe, phantom imaging with both PA and US contrasts is demonstrated. The results show that the probe has potential for endoscopic and intravascular imaging applications that require PA and US contrast with high resolution.

High-resolution MALDI mass spectrometry imaging of gallotannins and monoterpene glucosides in the root of Paeonia lactiflora

NASA Astrophysics Data System (ADS)

Li, Bin; Bhandari, Dhaka Ram; Römpp, Andreas; Spengler, Bernhard

2016-10-01

High-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI) at 10 μm pixel size was performed to unravel the spatio-chemical distribution of major secondary metabolites in the root of Paeonia lactiflora. The spatial distributions of two major classes of bioactive components, gallotannins and monoterpene glucosides, were investigated and visualized at the cellular level in tissue sections of P. lactiflora roots. Accordingly, other primary and secondary metabolites were imaged, including amino acids, carbohydrates, lipids and monoterpenes, indicating the capability of untargeted localization of metabolites by using high-resolution MSI platform. The employed AP-SMALDI MSI system provides significant technological advancement in the visualization of individual molecular species at the cellular level. In contrast to previous histochemical studies of tannins using unspecific staining reagents, individual gallotannin species were accurately localized and unequivocally discriminated from other phenolic components in the root tissues. High-quality ion images were obtained, providing significant clues for understanding the biosynthetic pathway of gallotannins and monoterpene glucosides and possibly helping to decipher the role of tannins in xylem cells differentiation and in the defence mechanisms of plants, as well as to investigate the interrelationship between tannins and lignins.

High-resolution MALDI mass spectrometry imaging of gallotannins and monoterpene glucosides in the root of Paeonia lactiflora.

PubMed

Li, Bin; Bhandari, Dhaka Ram; Römpp, Andreas; Spengler, Bernhard

2016-10-31

High-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI) at 10 μm pixel size was performed to unravel the spatio-chemical distribution of major secondary metabolites in the root of Paeonia lactiflora. The spatial distributions of two major classes of bioactive components, gallotannins and monoterpene glucosides, were investigated and visualized at the cellular level in tissue sections of P. lactiflora roots. Accordingly, other primary and secondary metabolites were imaged, including amino acids, carbohydrates, lipids and monoterpenes, indicating the capability of untargeted localization of metabolites by using high-resolution MSI platform. The employed AP-SMALDI MSI system provides significant technological advancement in the visualization of individual molecular species at the cellular level. In contrast to previous histochemical studies of tannins using unspecific staining reagents, individual gallotannin species were accurately localized and unequivocally discriminated from other phenolic components in the root tissues. High-quality ion images were obtained, providing significant clues for understanding the biosynthetic pathway of gallotannins and monoterpene glucosides and possibly helping to decipher the role of tannins in xylem cells differentiation and in the defence mechanisms of plants, as well as to investigate the interrelationship between tannins and lignins.

Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing.

PubMed

Yang, Qingbo; Wang, Hanzheng; Lan, Xinwei; Cheng, Baokai; Chen, Sisi; Shi, Honglan; Xiao, Hai; Ma, Yinfa

2015-02-01

pH sensing at the single-cell level without negatively affecting living cells is very important but still a remaining issue in the biomedical studies. A 70 μm reflection-mode fiber-optic micro-pH sensor was designed and fabricated by dip-coating thin layer of organically modified aerogel onto a tapered spherical probe head. A pH sensitive fluorescent dye 2', 7'-Bis (2-carbonylethyl)-5(6)-carboxyfluorescein (BCECF) was employed and covalently bonded within the aerogel networks. By tuning the alkoxide mixing ratio and adjusting hexamethyldisilazane (HMDS) priming procedure, the sensor can be optimized to have high stability and pH sensing ability. The in vitro real-time sensing capability was then demonstrated in a simple spectroscopic way, and showed linear measurement responses with a pH resolution up to an average of 0.049 pH unit within a narrow, but biological meaningful pH range of 6.12-7.81. Its novel characterizations of high spatial resolution, reflection mode operation, fast response and high stability, great linear response within biological meaningful pH range and high pH resolutions, make this novel pH probe a very cost-effective tool for chemical/biological sensing, especially within the single cell level research field.

Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing

PubMed Central

Yang, Qingbo; Wang, Hanzheng; Lan, Xinwei; Cheng, Baokai; Chen, Sisi; Shi, Honglan; Xiao, Hai; Ma, Yinfa

2014-01-01

pH sensing at the single-cell level without negatively affecting living cells is very important but still a remaining issue in the biomedical studies. A 70 μm reflection-mode fiber-optic micro-pH sensor was designed and fabricated by dip-coating thin layer of organically modified aerogel onto a tapered spherical probe head. A pH sensitive fluorescent dye 2′, 7′-Bis (2-carbonylethyl)-5(6)-carboxyfluorescein (BCECF) was employed and covalently bonded within the aerogel networks. By tuning the alkoxide mixing ratio and adjusting hexamethyldisilazane (HMDS) priming procedure, the sensor can be optimized to have high stability and pH sensing ability. The in vitro real-time sensing capability was then demonstrated in a simple spectroscopic way, and showed linear measurement responses with a pH resolution up to an average of 0.049 pH unit within a narrow, but biological meaningful pH range of 6.12–7.81. Its novel characterizations of high spatial resolution, reflection mode operation, fast response and high stability, great linear response within biological meaningful pH range and high pH resolutions, make this novel pH probe a very cost-effective tool for chemical/biological sensing, especially within the single cell level research field. PMID:25530670

Development of a High-Sensitivity Wireless Accelerometer for Structural Health Monitoring

PubMed Central

Zhu, Li; Fu, Yuguang; Chow, Raymond; Spencer, Billie F.; Park, Jong Woong; Mechitov, Kirill

2018-01-01

Structural health monitoring (SHM) is playing an increasingly important role in ensuring the safety of structures. A shift of SHM research away from traditional wired methods toward the use of wireless smart sensors (WSS) has been motivated by the attractive features of wireless smart sensor networks (WSSN). The progress achieved in Micro Electro-Mechanical System (MEMS) technologies and wireless data transmission, has extended the effectiveness and range of applicability of WSSNs. One of the most common sensors employed in SHM strategies is the accelerometer; however, most accelerometers in WSS nodes have inadequate resolution for measurement of the typical accelerations found in many SHM applications. In this study, a high-resolution and low-noise tri-axial digital MEMS accelerometer is incorporated in a next-generation WSS platform, the Xnode. In addition to meeting the acceleration sensing demands of large-scale civil infrastructure applications, this new WSS node provides powerful hardware and a robust software framework to enable edge computing that can deliver actionable information. Hardware and software integration challenges are presented, and the associate resolutions are discussed. The performance of the wireless accelerometer is demonstrated experimentally through comparison with high-sensitivity wired accelerometers. This new high-sensitivity wireless accelerometer will extend the use of WSSN to a broader class of SHM applications. PMID:29342102

High-Resolution Infrared and Electron-Diffraction Studies of Trimethylenecyclopropane ([3]-Radialene)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wright, Corey; Holmes, Joshua; Nibler, Joseph W.

2013-05-16

Combined high-resolution spectroscopic, electron-diffraction, and quantum theoretical methods are particularly advantageous for small molecules of high symmetry and can yield accurate structures that reveal subtle effects of electron delocalization on molecular bonds. The smallest of the radialene compounds, trimethylenecyclopropane, [3]-radialene, has been synthesized and examined in the gas phase by these methods. The first high-resolution infrared spectra have been obtained for this molecule of D3h symmetry, leading to an accurate B0 rotational constant value of 0.1378629(8) cm-1, within 0.5% of the value obtained from electronic structure calculations (density functional theory (DFT) B3LYP/cc-pVTZ). This result is employed in an analysis ofmore » electron-diffraction data to obtain the rz bond lengths (in Å): C-H = 1.072 (17), C-C = 1.437 (4), and C=C = 1.330 (4). The analysis does not lead to an accurate value of the HCH angle; however, from comparisons of theoretical and experimental angles for similar compounds, the theoretical prediction of 117.5° is believed to be reliable to within 2°. The effect of electron delocalization in radialene is to reduce the single C-C bond length by 0.07 Å compared to that in cyclopropane.« less

Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering

NASA Astrophysics Data System (ADS)

Lienemann, Philipp S.; Metzger, Stéphanie; Kiveliö, Anna-Sofia; Blanc, Alain; Papageorgiou, Panagiota; Astolfo, Alberto; Pinzer, Bernd R.; Cinelli, Paolo; Weber, Franz E.; Schibli, Roger; Béhé, Martin; Ehrbar, Martin

2015-05-01

Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical models. Since its discovery, micro-computed tomography (micro-CT) has excelled as powerful high-resolution technique for non-invasive assessment of newly formed bone tissue. However, micro-CT fails to provide spatiotemporal information on biological processes ongoing during bone regeneration. Conversely, due to the versatile applicability and cost-effectiveness, single photon emission computed tomography (SPECT) would be an ideal technique for assessing such biological processes with high sensitivity and for nuclear imaging comparably high resolution (<1 mm). Herein, we employ modular designed poly(ethylene glycol)-based hydrogels that release bone morphogenetic protein to guide the healing of critical sized calvarial bone defects. By combined in vivo longitudinal multi-pinhole SPECT and micro-CT evaluations we determine the spatiotemporal course of bone formation and remodeling within this synthetic hydrogel implant. End point evaluations by high resolution micro-CT and histological evaluation confirm the value of this approach to follow and optimize bone-inducing biomaterials.

Development of a High-Sensitivity Wireless Accelerometer for Structural Health Monitoring.

PubMed

Zhu, Li; Fu, Yuguang; Chow, Raymond; Spencer, Billie F; Park, Jong Woong; Mechitov, Kirill

2018-01-17

Structural health monitoring (SHM) is playing an increasingly important role in ensuring the safety of structures. A shift of SHM research away from traditional wired methods toward the use of wireless smart sensors (WSS) has been motivated by the attractive features of wireless smart sensor networks (WSSN). The progress achieved in Micro Electro-Mechanical System (MEMS) technologies and wireless data transmission, has extended the effectiveness and range of applicability of WSSNs. One of the most common sensors employed in SHM strategies is the accelerometer; however, most accelerometers in WSS nodes have inadequate resolution for measurement of the typical accelerations found in many SHM applications. In this study, a high-resolution and low-noise tri-axial digital MEMS accelerometer is incorporated in a next-generation WSS platform, the Xnode. In addition to meeting the acceleration sensing demands of large-scale civil infrastructure applications, this new WSS node provides powerful hardware and a robust software framework to enable edge computing that can deliver actionable information. Hardware and software integration challenges are presented, and the associate resolutions are discussed. The performance of the wireless accelerometer is demonstrated experimentally through comparison with high-sensitivity wired accelerometers. This new high-sensitivity wireless accelerometer will extend the use of WSSN to a broader class of SHM applications.

Serum metabolomics of slow vs. rapid motor progression Parkinson's disease: a pilot study.

PubMed

Roede, James R; Uppal, Karan; Park, Youngja; Lee, Kichun; Tran, Vilinh; Walker, Douglas; Strobel, Frederick H; Rhodes, Shannon L; Ritz, Beate; Jones, Dean P

2013-01-01

Progression of Parkinson's disease (PD) is highly variable, indicating that differences between slow and rapid progression forms could provide valuable information for improved early detection and management. Unfortunately, this represents a complex problem due to the heterogeneous nature of humans in regards to demographic characteristics, genetics, diet, environmental exposures and health behaviors. In this pilot study, we employed high resolution mass spectrometry-based metabolic profiling to investigate the metabolic signatures of slow versus rapidly progressing PD present in human serum. Archival serum samples from PD patients obtained within 3 years of disease onset were analyzed via dual chromatography-high resolution mass spectrometry, with data extraction by xMSanalyzer and used to predict rapid or slow motor progression of these patients during follow-up. Statistical analyses, such as false discovery rate analysis and partial least squares discriminant analysis, yielded a list of statistically significant metabolic features and further investigation revealed potential biomarkers. In particular, N8-acetyl spermidine was found to be significantly elevated in the rapid progressors compared to both control subjects and slow progressors. Our exploratory data indicate that a fast motor progression disease phenotype can be distinguished early in disease using high resolution mass spectrometry-based metabolic profiling and that altered polyamine metabolism may be a predictive marker of rapidly progressing PD.

Laboratory test results of the high speed optical tracking system for the Spaceborne Geodynamic Ranging System

NASA Technical Reports Server (NTRS)

Zagwodzki, Thomas W.; White, David L.

1987-01-01

The high speed, high resolution optical tracking system for the Spaceborne Geodynamic Ranging System employs a two-axis gimbaled pointing device that can operate from a Space Shuttle platform and can track multiple retroreflector ground targets with arcsec accuracy. Laboratory tests of the stepping characteristics of the pointing system for various step sizes and directions has shown arcsec repeatability with little wasted motion, overshoot, or ringing. The worst rms tracking jitter was 1 and 2 arcsec in the roll and pitch axes, respectively, at the maximum tracking rate of 2 deg/sec.

High temporal resolution fluorescence measurements of a mitochondrial dye for detection of early stage apoptosis

PubMed Central

Iyer, Divya; Ray, Rachel D.; Pappas, Dimitri

2013-01-01

In the present study, early stage apoptosis is explored with high temporal resolution. In addition to monitoring early apoptosis induction in single cells by ultrasensitive confocal fluorescence microscopy (UCFM), the mitochondrial proteins release kinetics was explored. The current study shows development and optimization of a novel, rapid apoptosis assay to explore the earliest changes in cells by the intrinsic apoptosis pathway. We show that early apoptotic changes in the mitochondria begin nearly simultaneously with the addition of an apoptosis-inducing drug, such as staurosporine. With a temporal resolution of five minutes, this non-invasive analytical technique can elucidate the earliest apoptotic events in living cells. Moreover, our results show that the mitochondrial inter-membrane proteins are not involved in the extrinsic pathway of Ramos cells mediated by an anti-CD95 antibody. Additional techniques such as light microscopy and flow cytometry were employed to confirm the results obtained by ultrasensitive confocal fluorescence microscopy. The results of this study help to understand the earliest mechanisms of apoptosis induction in cells, enabling new methods of drug testing and dose-response analyses. PMID:23831722

Multi-resolution Gabor wavelet feature extraction for needle detection in 3D ultrasound

NASA Astrophysics Data System (ADS)

Pourtaherian, Arash; Zinger, Svitlana; Mihajlovic, Nenad; de With, Peter H. N.; Huang, Jinfeng; Ng, Gary C.; Korsten, Hendrikus H. M.

2015-12-01

Ultrasound imaging is employed for needle guidance in various minimally invasive procedures such as biopsy guidance, regional anesthesia and brachytherapy. Unfortunately, a needle guidance using 2D ultrasound is very challenging, due to a poor needle visibility and a limited field of view. Nowadays, 3D ultrasound systems are available and more widely used. Consequently, with an appropriate 3D image-based needle detection technique, needle guidance and interventions may significantly be improved and simplified. In this paper, we present a multi-resolution Gabor transformation for an automated and reliable extraction of the needle-like structures in a 3D ultrasound volume. We study and identify the best combination of the Gabor wavelet frequencies. High precision in detecting the needle voxels leads to a robust and accurate localization of the needle for the intervention support. Evaluation in several ex-vivo cases shows that the multi-resolution analysis significantly improves the precision of the needle voxel detection from 0.23 to 0.32 at a high recall rate of 0.75 (gain 40%), where a better robustness and confidence were confirmed in the practical experiments.

Stennis Space Center Verification & Validation Capabilities

NASA Technical Reports Server (NTRS)

Pagnutti, Mary; Ryan, Robert E.; Holekamp, Kara; ONeal, Duane; Knowlton, Kelly; Ross, Kenton; Blonski, Slawomir

2005-01-01

Scientists within NASA s Applied Sciences Directorate have developed a well-characterized remote sensing Verification & Validation (V&V) site at the John C. Stennis Space Center (SSC). This site enables the in-flight characterization of satellite and airborne high spatial and moderate resolution remote sensing systems and their products. The smaller scale of the newer high resolution remote sensing systems allows scientists to characterize geometric, spatial, and radiometric data properties using a single V&V site. The targets and techniques used to characterize data from these newer systems can differ significantly from the techniques used to characterize data from the earlier, coarser spatial resolution systems. Scientists are also using the SSC V&V site to characterize thermal infrared systems and active lidar systems. SSC employs geodetic targets, edge targets, radiometric tarps, atmospheric monitoring equipment, and thermal calibration ponds to characterize remote sensing data products. The SSC Instrument Validation Lab is a key component of the V&V capability and is used to calibrate field instrumentation and to provide National Institute of Standards and Technology traceability. This poster presents a description of the SSC characterization capabilities and examples of calibration data.

Determining the maximum charging currents of lithium-ion cells for small charge quantities

NASA Astrophysics Data System (ADS)

Grimsmann, F.; Gerbert, T.; Brauchle, F.; Gruhle, A.; Parisi, J.; Knipper, M.

2017-10-01

In order to optimize the operating parameters of battery management systems for electric and hybrid vehicles, great interest has been shown in achieving the maximum permissible charging currents during recuperation, without causing a cell damage due to lithium plating, in relation to the temperature, charge quantity and state of charge. One method for determining these recuperation currents is measuring the cell thickness, where excessively high charging currents can be detected by an irreversible increase in thickness. It is not possible to measure particularly small charge quantities by employing mechanic dial indicators, which have a limited resolution of 1 μm. This is why we developed a measuring setup that has a resolution limit of less than 10 nm using a high-resolution contactless inductance sensor. Our results show that the permissible charging current I can be approximated in relation to the charge quantity x by a correlating function I =a /√{(x) } which is compliant with the Arrhenius law. Small charge quantities therefore have an optimization potential for energy recovery during recuperation.

Detection and signal amplification in zebrafish RNA FISH.

PubMed

Hauptmann, Giselbert; Lauter, Gilbert; Söll, Iris

2016-04-01

In situ hybridization (ISH) has become an invaluable tool for the detection of RNA in cells, tissues and organisms. Due to improvements in target and signal amplification and in probe design remarkable progress has been made concerning sensitivity, specificity and resolution of chromogenic and fluorescent ISH (FISH). These advancements allow for exquisite cellular and sub-cellular resolution and for detecting multiple RNA species at a time by multiplexing. In zebrafish (F)ISH non-enzymatic and enzymatic amplification systems have been employed to obtain enhanced signal intensities and signal-to-noise ratios. These amplification strategies include branched DNA-based RNAscope and in situ hybridization chain reaction (HCR) techniques, as well as alkaline phosphatase (AP)- and horseradish peroxidase (PO)-based immunoassays. For practical application, we provide proven multiplex FISH protocols for AP- and PO-based visualization of mRNAs at high resolution. The protocols take advantage of optimized tyramide signal amplification (TSA) conditions of the PO assay and long-lasting high signal-to-noise ratio of the AP reaction, thereby enabling detection of less abundant transcripts. Copyright © 2016 Elsevier Inc. All rights reserved.

Spirally-patterned pinhole arrays for long-term fluorescence cell imaging.

PubMed

Koo, Bon Ung; Kang, YooNa; Moon, SangJun; Lee, Won Gu

2015-11-07

Fluorescence cell imaging using a fluorescence microscope is an extensively used technique to examine the cell nucleus, internal structures, and other cellular molecules with fluorescence response time and intensity. However, it is difficult to perform high resolution cell imaging for a long period of time with this technique due to necrosis and apoptosis depending on the type and subcellular location of the damage caused by phototoxicity. A large number of studies have been performed to resolve this problem, but researchers have struggled to meet the challenge between cellular viability and image resolution. In this study, we employ a specially designed disc to reduce cell damage by controlling total fluorescence exposure time without deterioration of the image resolution. This approach has many advantages such as, the apparatus is simple, cost-effective, and easily integrated into the optical pathway through a conventional fluorescence microscope.

Ultra-narrow pulse generator with precision-adjustable pulse width

NASA Astrophysics Data System (ADS)

Fu, Zaiming; Liu, Hanglin

2018-05-01

In this paper, a novel ultra-narrow pulse generation approach is proposed. It is based on the decomposition and synthesis of pulse edges. Through controlling their relative delay, an ultra-narrow pulse could be generated. By employing field programmable gate array digital synthesis technology, the implemented pulse generator is with programmable ability. The amplitude of pulse signals is controlled by the radio frequency amplifiers and bias tees, and high precision can be achieved. More importantly, the proposed approach can break through the limitation of device's propagation delay and optimize the resolution and the accuracy of the pulse width significantly. The implemented pulse generator has two channels, whose minimum pulse width, frequency range, and amplitude range are 100 ps, 15 MHz-1.5 GHz, and 0.1 Vpp-1.8 Vpp, respectively. Both resolution of pulse width and channel delay are 1 ps, and amplitude resolution is 10 mVpp.

TES-Based X-Ray Microcalorimeter Performances Under AC Bias and FDM for Athena

NASA Technical Reports Server (NTRS)

Akamatsu, H.; Gottardi, L.; de Vries, C. P.; Adams, J. S.; Bandler, S. R.; Bruijn, M. P.; Chervenak, J. A.; Eckart, M. E.; Finkbeiner, F. M.; Gao, J. R.;

HS-SPM Mapping of Ferroelectric Domain Dynamics with Combined Nanoscale and Nanosecond Resolution

NASA Astrophysics Data System (ADS)

Polomoff, Nicholas Alexander

The unique properties of ferroelectric materials have been applied for a wide variety of device applications. In particular, properties such as spontaneous polarization and domain structure hysteresis at room temperature have rendered its application in nonvolatile memory devices such as FeRAMs. Along with the ever-present drive for smaller memory devices is the demand that they have increased operating speeds, longer retention times, lower power requirements and better overall reliability. It is therefore pertinent that further investigation of the dynamics, kinetics and mechanisms involved with ferroelectric domain polarization reversal at nanoscale lengths and temporal durations be conducted to optimize future ferroelectric based nonvolatile memory devices. Accordingly High Speed Piezoforce Microscopy (HSPFM) will be employed to directly investigate and observe the dynamic nucleation and growth progression of ferroelectric domain polarization reversal processes in thin epitaxial deposited PZT films. The capabilities of HSPFM will allow for in-situ direct observation of nascent dynamic domain polarization reversal events with nanoscale resolution. Correlations and characterization of the thin ferroelectric film samples will be made based on the observed polarization reversal dynamics and switching mechanism with respect to their varying strain states, compositions, and/or orientations. Electrical pulsing schemes will also be employed to enhance the HSPFM procedure to achieve nanoscale temporal resolution of nascent domain nucleation and growth events. A unique pulsing approach is also proposed, and tested, to improve power consumption during switching. Finally, artificial defects will be introduced into the PZT thin film by fabricating arrays of indentations with different shapes and loads. These controlled indents will result in the introduction of different stress states of compression and tension into the ferroelectric thin film. It is hypothesized that these different stress states will have a dramatic effect upon the polarization reversal process, domain nucleation and growth dynamics, as well as the device's overall performance. It is the aim of the research presented in this dissertation to leverage the superior lateral and temporal resolution of the HSPFM technique to observe the influence that a variety of different variables have upon polarization reversal and dynamic ferroelectric domain behavior in attempt to propose conventions in which such variables can be employed for the development of high functioning and overall better operating ferroelectric based devices.

Application of Radioxenon Stack Emission Data in High-Resolution Atmospheric Transport Modelling

NASA Astrophysics Data System (ADS)

Kusmierczyk-Michulec, J.; Schoeppner, M.; Kalinowski, M.; Bourgouin, P.; Kushida, N.; Barè, J.

2017-12-01

The Comprehensive Nuclear-Test-Ban Treaty Organisation (CTBTO) has developed the capability to run high-resolution atmospheric transport modelling by employing WRF and Flexpart-WRF. This new capability is applied to simulate the impact of stack emission data on simulated concentrations and how the availability of such data improves the overall accuracy of atmospheric transport modelling. The presented case study focuses on xenon-133 emissions from IRE, a medical isotope production facility in Belgium, and air concentrations detected at DEX33, a monitoring station close to Freiburg, Germany. The CTBTO is currently monitoring the atmospheric concentration of xenon-133 at 25 stations and will further expand the monitoring efforts to 40 stations worldwide. The incentive is the ability to detect xenon-133 that has been produced and released from a nuclear explosion. A successful detection can be used to prove the nuclear nature of an explosion and even support localization efforts. However, xenon-133 is also released from nuclear power plants and to a larger degree from medical isotope production facilities. The availability of stack emission data in combination with atmospheric transport modelling can greatly facilitate the understanding of xenon-133 concentrations detected at monitoring stations to distinguish between xenon-133 that has been emitted from a nuclear explosion and from civilian sources. Newly available stack emission data is used with a high-resolution version of the Flexpart atmospheric transport model, namely Flexpart-WRF, to assess the impact of the emissions on the detected concentrations and the advantage gained from the availability of such stack emission data. The results are analyzed with regard to spatial and time resolution of the high-resolution model and in comparison to conventional atmospheric transport models with and without stack emission data.

High Resolution PTR-TOFMS: A New Instrument for Organic Compound Measurements

NASA Astrophysics Data System (ADS)

Hansel, A.; Graus, M.; Mueller, M.; Wisthaler, A.

2007-12-01

Over the last decade proton transfer reaction mass spectrometry (PTR-MS) has become very popular in many scientific fields. PTR-MS allows for the quantitative detection of volatile organic compounds (VOCs) at pptv-level virtually in real time. Monitoring of VOCs with a time resolution of typically a second per compound has, for instance, enabled the tracking of pollution plumes by air-borne measurements, thus revealing the photo- chemical fate of pollutants. It has also been employed in direct eddy covariant flux measurements. This rapidity, however, has been achieved at the cost of the number of compounds to be analyzed and compound selectivity. Conventional PTR-MS can, for example, not distinguish between hydrocarbons and their oxygenated isobaric species, e.g. between naphthalene and octanal or between isoprene and furan. In a mass range up to 200 Dalton, such a task would require a mass resolving power of 5500. The use of a time of flight (TOF) instead of a quadrupole mass analyzer in PTR-MS provides a sufficient high mass resolution to identify the atomic composition of product ions by their exact mass and their characteristic isotope patterns. In addition PTR-TOF-MS can record full mass spectra within a fraction of a second which is a dramatically increase in duty cycle. At the University of Innsbruck a high resolution PTR-TOFMS has recently been developed, coupling a PTR-ion source and a high resolution TOFMS. We achieved a mass resolving power of 6000 (FWHM), and a detection limit of tens to a few hundreds of pptv if integrating mass spectra for one minute. First results and future directions will be discussed in this paper.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bolotnikov, A. E.; Camarda, G. S.; Cui, Y.

Following our successful demonstration of the position-sensitive virtual Frisch-grid detectors, we investigated the feasibility of using high-granularity position sensing to correct response non-uniformities caused by the crystal defects in CdZnTe (CZT) pixelated detectors. The development of high-granularity detectors able to correct response non-uniformities on a scale comparable to the size of electron clouds opens the opportunity of using unselected off-the-shelf CZT material, whilst still assuring high spectral resolution for the majority of the detectors fabricated from an ingot. Here, we present the results from testing 3D position-sensitive 15×15×10 mm 3 pixelated detectors, fabricated with conventional pixel patterns with progressively smallermore » pixel sizes: 1.4, 0.8, and 0.5 mm. We employed the readout system based on the H3D front-end multi-channel ASIC developed by BNL's Instrumentation Division in collaboration with the University of Michigan. We use the sharing of electron clouds among several adjacent pixels to measure locations of interaction points with sub-pixel resolution. By using the detectors with small-pixel sizes and a high probability of the charge-sharing events, we were able to improve their spectral resolutions in comparison to the baseline levels, measured for the 1.4-mm pixel size detectors with small fractions of charge-sharing events. These results demonstrate that further enhancement of the performance of CZT pixelated detectors and reduction of costs are possible by using high spatial-resolution position information of interaction points to correct the small-scale response non-uniformities caused by crystal defects present in most devices.« less

Quantifying the ultrastructure of carotid arteries using high-resolution micro-diffusion tensor imaging—comparison of intact versus open cut tissue

NASA Astrophysics Data System (ADS)

Salman Shahid, Syed; Gaul, Robert T.; Kerskens, Christian; Flamini, Vittoria; Lally, Caitríona

2017-12-01

Diffusion magnetic resonance imaging (dMRI) can provide insights into the microstructure of intact arterial tissue. The current study employed high magnetic field MRI to obtain ultra-high resolution dMRI at an isotropic voxel resolution of 117 µm3 in less than 2 h of scan time. A parameter selective single shell (128 directions) diffusion-encoding scheme based on Stejskel-Tanner sequence with echo-planar imaging (EPI) readout was used. EPI segmentation was used to reduce the echo time (TE) and to minimise the susceptibility-induced artefacts. The study utilised the dMRI analysis with diffusion tensor imaging (DTI) framework to investigate structural heterogeneity in intact arterial tissue and to quantify variations in tissue composition when the tissue is cut open and flattened. For intact arterial samples, the region of interest base comparison showed significant differences in fractional anisotropy and mean diffusivity across the media layer (p  <  0.05). For open cut flat samples, DTI based directionally invariant indices did not show significant differences across the media layer. For intact samples, fibre tractography based indices such as calculated helical angle and fibre dispersion showed near circumferential alignment and a high degree of fibre dispersion, respectively. This study demonstrates the feasibility of fast dMRI acquisition with ultra-high spatial and angular resolution at 7 T. Using the optimised sequence parameters, this study shows that DTI based markers are sensitive to local structural changes in intact arterial tissue samples and these markers may have clinical relevance in the diagnosis of atherosclerosis and aneurysm.

10 CFR 850.5 - Dispute resolution.

Code of Federal Regulations, 2010 CFR

2010-01-01

... accept a petition from a worker unless the worker requested the responsible employer to correct the violation, and the responsible employer refused or failed to take corrective action within a reasonable time...

A compact high-definition low-cost digital stereoscopic video camera for rapid robotic surgery development.

PubMed

Carlson, Jay; Kowalczuk, Jędrzej; Psota, Eric; Pérez, Lance C

2012-01-01

Robotic surgical platforms require vision feedback systems, which often consist of low-resolution, expensive, single-imager analog cameras. These systems are retooled for 3D display by simply doubling the cameras and outboard control units. Here, a fully-integrated digital stereoscopic video camera employing high-definition sensors and a class-compliant USB video interface is presented. This system can be used with low-cost PC hardware and consumer-level 3D displays for tele-medical surgical applications including military medical support, disaster relief, and space exploration.

U-Shaped and Surface Functionalized Polymer Optical Fiber Probe for Glucose Detection.

PubMed

Azkune, Mikel; Ruiz-Rubio, Leire; Aldabaldetreku, Gotzon; Arrospide, Eneko; Pérez-Álvarez, Leyre; Bikandi, Iñaki; Zubia, Joseba; Vilas-Vilela, Jose Luis

2017-12-25

In this work we show an optical fiber evanescent wave absorption probe for glucose detection in different physiological media. High selectivity is achieved by functionalizing the surface of an only-core poly(methyl methacrylate) (PMMA) polymer optical fiber with phenilboronic groups, and enhanced sensitivity by using a U-shaped geometry. Employing a supercontinuum light source and a high-resolution spectrometer, absorption measurements are performed in the broadband visible light spectrum. Experimental results suggest the feasibility of such a fiber probe as a low-cost and selective glucose detector.

An automated microfluidic platform for C. elegans embryo arraying, phenotyping, and long-term live imaging

NASA Astrophysics Data System (ADS)

Cornaglia, Matteo; Mouchiroud, Laurent; Marette, Alexis; Narasimhan, Shreya; Lehnert, Thomas; Jovaisaite, Virginija; Auwerx, Johan; Gijs, Martin A. M.

2015-05-01

Studies of the real-time dynamics of embryonic development require a gentle embryo handling method, the possibility of long-term live imaging during the complete embryogenesis, as well as of parallelization providing a population’s statistics, while keeping single embryo resolution. We describe an automated approach that fully accomplishes these requirements for embryos of Caenorhabditis elegans, one of the most employed model organisms in biomedical research. We developed a microfluidic platform which makes use of pure passive hydrodynamics to run on-chip worm cultures, from which we obtain synchronized embryo populations, and to immobilize these embryos in incubator microarrays for long-term high-resolution optical imaging. We successfully employ our platform to investigate morphogenesis and mitochondrial biogenesis during the full embryonic development and elucidate the role of the mitochondrial unfolded protein response (UPRmt) within C. elegans embryogenesis. Our method can be generally used for protein expression and developmental studies at the embryonic level, but can also provide clues to understand the aging process and age-related diseases in particular.

Large area and deep sub-wavelength interference lithography employing odd surface plasmon modes.

PubMed

Liu, Liqin; Luo, Yunfei; Zhao, Zeyu; Zhang, Wei; Gao, Guohan; Zeng, Bo; Wang, Changtao; Luo, Xiangang

2016-07-28

In this paper, large area and deep sub-wavelength interference patterns are realized experimentally by using odd surface plasmon modes in the metal/insulator/metal structure. Theoretical investigation shows that the odd modes possesses much higher transversal wave vector and great inhibition of tangential electric field components, facilitating surface plasmon interference fringes with high resolution and contrast in the measure of electric field intensity. Interference resist patterns with 45 nm (∼λ/8) half-pitch, 50 nm depth, and area size up to 20 mm × 20 mm were obtained by using 20 nm Al/50 nm photo resist/50 nm Al films with greatly reduced surface roughness and 180 nm pitch exciting grating fabricated with conventional laser interference lithography. Much deeper resolution down to 19.5 nm is also feasible by decreasing the thickness of PR. Considering that no requirement of expensive EBL or FIB tools are employed, it provides a cost-effective way for large area and nano-scale fabrication.

Information recovery through image sequence fusion under wavelet transformation

NASA Astrophysics Data System (ADS)

He, Qiang

2010-04-01

Remote sensing is widely applied to provide information of areas with limited ground access with applications such as to assess the destruction from natural disasters and to plan relief and recovery operations. However, the data collection of aerial digital images is constrained by bad weather, atmospheric conditions, and unstable camera or camcorder. Therefore, how to recover the information from the low-quality remote sensing images and how to enhance the image quality becomes very important for many visual understanding tasks, such like feature detection, object segmentation, and object recognition. The quality of remote sensing imagery can be improved through meaningful combination of the employed images captured from different sensors or from different conditions through information fusion. Here we particularly address information fusion to remote sensing images under multi-resolution analysis in the employed image sequences. The image fusion is to recover complete information by integrating multiple images captured from the same scene. Through image fusion, a new image with high-resolution or more perceptive for human and machine is created from a time series of low-quality images based on image registration between different video frames.

Simulations of Madden-Julian Oscillation in High Resolution Atmospheric General Circulation Model

NASA Astrophysics Data System (ADS)

Deng, Liping; Stenchikov, Georgiy; McCabe, Matthew; Bangalath, HamzaKunhu; Raj, Jerry; Osipov, Sergey

2014-05-01

The simulation of tropical signals, especially the Madden-Julian Oscillation (MJO), is one of the major deficiencies in current numerical models. The unrealistic features in the MJO simulations include the weak amplitude, more power at higher frequencies, displacement of the temporal and spatial distributions, eastward propagation speed being too fast, and a lack of coherent structure for the eastward propagation from the Indian Ocean to the Pacific (e.g., Slingo et al. 1996). While some improvement in simulating MJO variance and coherent eastward propagation has been attributed to model physics, model mean background state and air-sea interaction, studies have shown that the model resolution, especially for higher horizontal resolution, may play an important role in producing a more realistic simulation of MJO (e.g., Sperber et al. 2005). In this study, we employ unique high-resolution (25-km) simulations conducted using the Geophysical Fluid Dynamics Laboratory global High Resolution Atmospheric Model (HIRAM) to evaluate the MJO simulation against the European Center for Medium-range Weather Forecasts (ECMWF) Interim re-analysis (ERAI) dataset. We specifically focus on the ability of the model to represent the MJO related amplitude, spatial distribution, eastward propagation, and horizontal and vertical structures. Additionally, as the HIRAM output covers not only an historic period (1979-2012) but also future period (2012-2050), the impact of future climate change related to the MJO is illustrated. The possible changes in intensity and frequency of extreme weather and climate events (e.g., strong wind and heavy rainfall) in the western Pacific, the Indian Ocean and the Middle East North Africa (MENA) region are highlighted.

Ministerial Resolution No. 87, 1989. [Summary].

PubMed

1989-01-01

A Ministerial Resolution passed in 1989 in Kuwait amended private sector recruitment regulations to encourage the employment of Kuwaiti and other Gulf nationals. The resolution gave local labor authorities the responsibility of issuing work permits. Foreign workers can gain a work permit is 1) no Kuwaiti or Gulf nationals are available, 2) the recruitment can be justified, and 3) the foreign worker's skills conform with the business activity and needs of the enterprise seeking the permit. Workers hired without a permit will be deported at the employer's expense, and the employer will be banned from receiving any other work permits for up to 6 months. In addition, permit holders can only work for the employer who acquired the permit on their behalf. Self-employed foreign workers will receive a permit when they are associated with a local company and receive a license to work on an independent basis. Aliens who have been practicing an activity for at least 10 years, however, and are over age 30 and have recognized domicile will be issued a 3-year renewable permit. Work permits are not transferrable to another employer for 3 years from the date of issue. Government contractors, however, will receive permits for the entire duration of a project, after which time the foreign workers will be repatriated unless they are transferred to another employer working on a similar government project. The Ministry of Labour may reject an application for a work permit, its renewal, or transfer and may cancel a work permit at any time. Repatriation costs are normally paid by the employer, unless the termination was initiated on disciplinary grounds or for failure to comply with contract terms or labor regulations. In these cases, the workers will pay their own repatriation costs.

Multiresolution analysis of characteristic length scales with high-resolution topographic data

NASA Astrophysics Data System (ADS)

Sangireddy, Harish; Stark, Colin P.; Passalacqua, Paola

2017-07-01

Characteristic length scales (CLS) define landscape structure and delimit geomorphic processes. Here we use multiresolution analysis (MRA) to estimate such scales from high-resolution topographic data. MRA employs progressive terrain defocusing, via convolution of the terrain data with Gaussian kernels of increasing standard deviation, and calculation at each smoothing resolution of (i) the probability distributions of curvature and topographic index (defined as the ratio of slope to area in log scale) and (ii) characteristic spatial patterns of divergent and convergent topography identified by analyzing the curvature of the terrain. The MRA is first explored using synthetic 1-D and 2-D signals whose CLS are known. It is then validated against a set of MARSSIM (a landscape evolution model) steady state landscapes whose CLS were tuned by varying hillslope diffusivity and simulated noise amplitude. The known CLS match the scales at which the distributions of topographic index and curvature show scaling breaks, indicating that the MRA can identify CLS in landscapes based on the scaling behavior of topographic attributes. Finally, the MRA is deployed to measure the CLS of five natural landscapes using meter resolution digital terrain model data. CLS are inferred from the scaling breaks of the topographic index and curvature distributions and equated with (i) small-scale roughness features and (ii) the hillslope length scale.

REFMAC5 for the refinement of macromolecular crystal structures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Murshudov, Garib N., E-mail: garib@ysbl.york.ac.uk; Skubák, Pavol; Lebedev, Andrey A.

The general principles behind the macromolecular crystal structure refinement program REFMAC5 are described. This paper describes various components of the macromolecular crystallographic refinement program REFMAC5, which is distributed as part of the CCP4 suite. REFMAC5 utilizes different likelihood functions depending on the diffraction data employed (amplitudes or intensities), the presence of twinning and the availability of SAD/SIRAS experimental diffraction data. To ensure chemical and structural integrity of the refined model, REFMAC5 offers several classes of restraints and choices of model parameterization. Reliable models at resolutions at least as low as 4 Å can be achieved thanks to low-resolution refinement toolsmore » such as secondary-structure restraints, restraints to known homologous structures, automatic global and local NCS restraints, ‘jelly-body’ restraints and the use of novel long-range restraints on atomic displacement parameters (ADPs) based on the Kullback–Leibler divergence. REFMAC5 additionally offers TLS parameterization and, when high-resolution data are available, fast refinement of anisotropic ADPs. Refinement in the presence of twinning is performed in a fully automated fashion. REFMAC5 is a flexible and highly optimized refinement package that is ideally suited for refinement across the entire resolution spectrum encountered in macromolecular crystallography.« less

Computational analysis of high resolution unsteady airloads for rotor aeroacoustics

NASA Technical Reports Server (NTRS)

Quackenbush, Todd R.; Lam, C.-M. Gordon; Wachspress, Daniel A.; Bliss, Donald B.

1994-01-01

The study of helicopter aerodynamic loading for acoustics applications requires the application of efficient yet accurate simulations of the velocity field induced by the rotor's vortex wake. This report summarizes work to date on the development of such an analysis, which builds on the Constant Vorticity Contour (CVC) free wake model, previously implemented for the study of vibratory loading in the RotorCRAFT computer code. The present effort has focused on implementation of an airload reconstruction approach that computes high resolution airload solutions of rotor/rotor-wake interactions required for acoustics computations. Supplementary efforts on the development of improved vortex core modeling, unsteady aerodynamic effects, higher spatial resolution of rotor loading, and fast vortex wake implementations have substantially enhanced the capabilities of the resulting software, denoted RotorCRAFT/AA (AeroAcoustics). Results of validation calculations using recently acquired model rotor data show that by employing airload reconstruction it is possible to apply the CVC wake analysis with temporal and spatial resolution suitable for acoustics applications while reducing the computation time required by one to two orders of magnitude relative to that required by direct calculations. Promising correlation with this body of airload and noise data has been obtained for a variety of rotor configurations and operating conditions.

Single Anisotropic 3-D MR Image Upsampling via Overcomplete Dictionary Trained From In-Plane High Resolution Slices.

PubMed

Jia, Yuanyuan; He, Zhongshi; Gholipour, Ali; Warfield, Simon K

2016-11-01

In magnetic resonance (MR), hardware limitation, scanning time, and patient comfort often result in the acquisition of anisotropic 3-D MR images. Enhancing image resolution is desired but has been very challenging in medical image processing. Super resolution reconstruction based on sparse representation and overcomplete dictionary has been lately employed to address this problem; however, these methods require extra training sets, which may not be always available. This paper proposes a novel single anisotropic 3-D MR image upsampling method via sparse representation and overcomplete dictionary that is trained from in-plane high resolution slices to upsample in the out-of-plane dimensions. The proposed method, therefore, does not require extra training sets. Abundant experiments, conducted on simulated and clinical brain MR images, show that the proposed method is more accurate than classical interpolation. When compared to a recent upsampling method based on the nonlocal means approach, the proposed method did not show improved results at low upsampling factors with simulated images, but generated comparable results with much better computational efficiency in clinical cases. Therefore, the proposed approach can be efficiently implemented and routinely used to upsample MR images in the out-of-planes views for radiologic assessment and postacquisition processing.

Molecular understanding of mutagenicity using potential energy methods. Progress report, July 1, 1992--September 30, 1993

DOE Office of Scientific and Technical Information (OSTI.GOV)

Broyde, S.; Shapiro, R.

1993-09-01

Our objective has been to elucidate on a molecular level, at atomic resolution, the structures of DNAs modified by highly mutagenic aromatic amines and hydrocarbons. The underlying hypothesis is that DNA replicates with reduced fidelity when its normal right-handed B-structure is altered, and one result is a higher mutation rate. This change in structure may occur normally at a low incidence but it may be enhanced greatly after covalent modification by a mutagenic substance. The methods that we use to elucidate structures are computational, but we keep in close contact with experimental developments, and we incorporate data from NMR studiesmore » in our calculations when they are available. X-ray and low resolution spectroscopic studies have not succeeded in producing atomic resolution views of mutagen and carcinogen-oligonucleotide adducts. Even the high resolution NMR method cannot alone yield molecular views, though it does so in combination with our computations. The specific methods that we employ are minimized potential energy calculations using the torsion angle space molecular mechanics program DUPLEX to yield static views. Molecular dynamics simulations of static structures with solvent and salt can be carried out with the program AMBER; this yields mobile views in a medium that mimics aspects of the natural aqueous environment of the cell.« less

Stennis Space Center Verification & Validation Capabilities

NASA Technical Reports Server (NTRS)

Pagnutti, Mary; Ryan, Robert E.; Holekamp, Kara; O'Neal, Duane; Knowlton, Kelly; Ross, Kenton; Blonski, Slawomir

2007-01-01

Scientists within NASA#s Applied Research & Technology Project Office (formerly the Applied Sciences Directorate) have developed a well-characterized remote sensing Verification & Validation (V&V) site at the John C. Stennis Space Center (SSC). This site enables the in-flight characterization of satellite and airborne high spatial resolution remote sensing systems and their products. The smaller scale of the newer high resolution remote sensing systems allows scientists to characterize geometric, spatial, and radiometric data properties using a single V&V site. The targets and techniques used to characterize data from these newer systems can differ significantly from the techniques used to characterize data from the earlier, coarser spatial resolution systems. Scientists have used the SSC V&V site to characterize thermal infrared systems. Enhancements are being considered to characterize active lidar systems. SSC employs geodetic targets, edge targets, radiometric tarps, atmospheric monitoring equipment, and thermal calibration ponds to characterize remote sensing data products. Similar techniques are used to characterize moderate spatial resolution sensing systems at selected nearby locations. The SSC Instrument Validation Lab is a key component of the V&V capability and is used to calibrate field instrumentation and to provide National Institute of Standards and Technology traceability. This poster presents a description of the SSC characterization capabilities and examples of calibration data.

Effects of important parameters variations on computing eigenspace-based minimum variance weights for ultrasound tissue harmonic imaging

NASA Astrophysics Data System (ADS)

Haji Heidari, Mehdi; Mozaffarzadeh, Moein; Manwar, Rayyan; Nasiriavanaki, Mohammadreza

2018-02-01

In recent years, the minimum variance (MV) beamforming has been widely studied due to its high resolution and contrast in B-mode Ultrasound imaging (USI). However, the performance of the MV beamformer is degraded at the presence of noise, as a result of the inaccurate covariance matrix estimation which leads to a low quality image. Second harmonic imaging (SHI) provides many advantages over the conventional pulse-echo USI, such as enhanced axial and lateral resolutions. However, the low signal-to-noise ratio (SNR) is a major problem in SHI. In this paper, Eigenspace-based minimum variance (EIBMV) beamformer has been employed for second harmonic USI. The Tissue Harmonic Imaging (THI) is achieved by Pulse Inversion (PI) technique. Using the EIBMV weights, instead of the MV ones, would lead to reduced sidelobes and improved contrast, without compromising the high resolution of the MV beamformer (even at the presence of a strong noise). In addition, we have investigated the effects of variations of the important parameters in computing EIBMV weights, i.e., K, L, and δ, on the resolution and contrast obtained in SHI. The results are evaluated using numerical data (using point target and cyst phantoms), and the proper parameters of EIBMV are indicated for THI.

"No! The lambs can stay out because they got cozies": constructive and destructive sibling conflict, pretend play, and social understanding.

PubMed

Howe, Nina; Rinaldi, Christina M; Jennings, Melissa; Petrakos, Harriet

2002-01-01

Associations among constructive and destructive sibling conflict, pretend play, internal state language, and sibling relationship quality were investigated in 40 middle-class dyads with a kindergarten-age child (M age = 5.7 years). In 20 dyads the sibling was older (M age = 7.1 years) and in 20 dyads the sibling was younger (M age = 3.6 years). Dyads were videotaped playing with a farm set for 15 min; transcribed sessions were coded for (1) five types of conflict issues; (2) constructive, destructive, and passive resolution strategies; and (3) verbal and physical aggression. Measures of pretend play enactment, low- and high-level pretense negotiation strategies, and internal state language were also based on the transcripts. The Sibling Behavior and Feelings Questionnaire was used to assess both siblings' perceptions of sibling relationship quality. Findings revealed that conflict issues, aggression, and internal state language were associated with specific resolution strategies. Associations were evident between conflict issues and resolutions. Moreover, conflict issues and resolutions were associated with (1) relationship quality, (2) high-level pretense negotiation, and (3) internal state language employed in both play and conflict. Findings are discussed in light of recent theory on developmental processes operating within children's relationships.

Resolution enhancement in MR spectroscopy of red bone marrow fat via intermolecular double-quantum coherences

NASA Astrophysics Data System (ADS)

Bao, Jianfeng; Cui, Xiaohong; Huang, Yuqing; Zhong, Jianhui; Chen, Zhong

2015-08-01

High-resolution 1H magnetic resonance spectroscopy (MRS) is generally inaccessible in red bone marrow (RBM) tissues using conventional MRS techniques. This is because signal from these tissues suffers from severe inhomogeneity in the main static B0 field originated from the intrinsic honeycomb structures in trabecular bone. One way to reduce effects of B0 field inhomogeneity is by using the intermolecular double quantum coherence (iDQC) technique, which has been shown in other systems to obtain signals insensitive to B0 field inhomogeneity. In the present study, we employed an iDQC approach to enhance the spectral resolution of RBM. The feasibility and performance of this method for achieving high resolution MRS was verified by experiments on phantoms and pig vertebral bone samples. Unsaturated fatty acid peaks which overlap in the conventional MRS were well resolved and identified in the iDQC spectrum. Quantitative comparison of fractions of three types of fatty acids was performed between iDQC spectra on the in situ RMB and conventional MRS on the extracted fat from the same RBM. Observations of unsaturated fatty acids with iDQC MRS may provide valuable information and may hold potential in diagnosis of diseases such as obesity, diabetes, and leukemia.

High Resolution Investigation of the Ethane Spectrum at 7 μ

NASA Astrophysics Data System (ADS)

Brown, Linda R.; Sung, K.; Di Lauro, C.; Lattanzi, F.; Vander Auwera, J.; Mantz, A. W.; Smith, M. A. H.

2010-10-01

A new theoretical analysis of the ethane spectrum between 1330 and 1610 cm-1 has been undertaken in order to create the first line-by-line database of molecular parameters for this spectral region. For this, high resolution spectra were obtained at room and cold (130 K) temperatures with two Bruker Fourier transform spectrometers (at 0.002 cm-1 resolution in Brussels and at 0.003 cm-1 resolution in Pasadena). Over 5000 lines were assigned to five bands in the region: v6, v8, v4+v12 and 2v4+v9 cold bands, and one hot band (v4+v8-v4). This new study employed a much improved theoretical Hamiltonian to reproduce the very complicated spectral structures resulting from numerous interactions between these vibrational modes. This advancement has enabled us to provide a quantum mechanical prediction of line positions and intensities of C2H6 at 7 micron long needed for remote sensing of outer planets and Titan. Two manuscripts are in preparation. Part of the research described in this paper was performed at the Jet Propulsion Laboratory, California Institute of Technology, Connecticut College, and NASA Langley under contract with the National Aeronautics and Space Administration, and with funding from FRS-FNRS in Belgium.

Numerical study of low-current steady arcs

NASA Technical Reports Server (NTRS)

Kim, S. C.; Nagamatsu, H. T.

1992-01-01

The development of a high-efficiency CW YLF laser doped with Er,Tm,Ho: and featuring a strongly focusing resonator that collects a high density of pump power on the active crystal is described. The emission is investigated at 2.06 microns and a tuning range both at liquid-nitrogen (77 K) and at dry-ice (210 K) temperature. The noise characteristics and the long-term power stability of the laser is studied with an eye to employing this source for high-resolution spectroscopy in the 2-micron wavelength region. The detection of several absorption lines of NH3 at low pressure is described. The output power of the laser as a function of the power impinging on the crystal for different transmission of the output mirror is illustrated. The best result obtained is 1.46 W output for 3.2 W of argon pump. The minimum threshold achieved is 3.5 mW with a 1-percent transmission mirror. It is concluded that it is possible to develop a highly efficient Ho:YLF laser featuring low noise and sufficient tunability for high-resolution spectroscopy in the 2-micron region.

A high sensitivity 20Mfps CMOS image sensor with readout speed of 1Tpixel/sec for visualization of ultra-high speed phenomena

NASA Astrophysics Data System (ADS)

Kuroda, R.; Sugawa, S.

2017-02-01

Ultra-high speed (UHS) CMOS image sensors with on-chop analog memories placed on the periphery of pixel array for the visualization of UHS phenomena are overviewed in this paper. The developed UHS CMOS image sensors consist of 400H×256V pixels and 128 memories/pixel, and the readout speed of 1Tpixel/sec is obtained, leading to 10 Mfps full resolution video capturing with consecutive 128 frames, and 20 Mfps half resolution video capturing with consecutive 256 frames. The first development model has been employed in the high speed video camera and put in practical use in 2012. By the development of dedicated process technologies, photosensitivity improvement and power consumption reduction were simultaneously achieved, and the performance improved version has been utilized in the commercialized high-speed video camera since 2015 that offers 10 Mfps with ISO16,000 photosensitivity. Due to the improved photosensitivity, clear images can be captured and analyzed even under low light condition, such as under a microscope as well as capturing of UHS light emission phenomena.

The development and validation of command schedules for SeaWiFS

NASA Astrophysics Data System (ADS)

Woodward, Robert H.; Gregg, Watson W.; Patt, Frederick S.

1994-11-01

An automated method for developing and assessing spacecraft and instrument command schedules is presented for the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) project. SeaWiFS is to be carried on the polar-orbiting SeaStar satellite in 1995. The primary goal of the SeaWiFS mission is to provide global ocean chlorophyll concentrations every four days by employing onboard recorders and a twice-a-day data downlink schedule. Global Area Coverage (GAC) data with about 4.5 km resolution will be used to produce the global coverage. Higher resolution (1.1 km resolution) Local Area Coverage (LAC) data will also be recorded to calibrate the sensor. In addition, LAC will be continuously transmitted from the satellite and received by High Resolution Picture Transmission (HRPT) stations. The methods used to generate commands for SeaWiFS employ numerous hierarchical checks as a means of maximizing coverage of the Earth's surface and fulfilling the LAC data requirements. The software code is modularized and written in Fortran with constructs to mirror the pre-defined mission rules. The overall method is specifically developed for low orbit Earth-observing satellites with finite onboard recording capabilities and regularly scheduled data downlinks. Two software packages using the Interactive Data Language (IDL) for graphically displaying and verifying the resultant command decisions are presented. Displays can be generated which show portions of the Earth viewed by the sensor and spacecraft sub-orbital locations during onboard calibration activities. An IDL-based interactive method of selecting and testing LAC targets and calibration activities for command generation is also discussed.

High-resolution mapping of bifurcations in nonlinear biochemical circuits

NASA Astrophysics Data System (ADS)

Genot, A. J.; Baccouche, A.; Sieskind, R.; Aubert-Kato, N.; Bredeche, N.; Bartolo, J. F.; Taly, V.; Fujii, T.; Rondelez, Y.

2016-08-01

Analog molecular circuits can exploit the nonlinear nature of biochemical reaction networks to compute low-precision outputs with fewer resources than digital circuits. This analog computation is similar to that employed by gene-regulation networks. Although digital systems have a tractable link between structure and function, the nonlinear and continuous nature of analog circuits yields an intricate functional landscape, which makes their design counter-intuitive, their characterization laborious and their analysis delicate. Here, using droplet-based microfluidics, we map with high resolution and dimensionality the bifurcation diagrams of two synthetic, out-of-equilibrium and nonlinear programs: a bistable DNA switch and a predator-prey DNA oscillator. The diagrams delineate where function is optimal, dynamics bifurcates and models fail. Inverse problem solving on these large-scale data sets indicates interference from enzymatic coupling. Additionally, data mining exposes the presence of rare, stochastically bursting oscillators near deterministic bifurcations.

Long-term C. elegans immobilization enables high resolution developmental studies in vivo.

PubMed

Berger, Simon; Lattmann, Evelyn; Aegerter-Wilmsen, Tinri; Hengartner, Michael; Hajnal, Alex; deMello, Andrew; Casadevall I Solvas, Xavier

2018-05-01

Live-imaging of C. elegans is essential for the study of conserved cellular pathways (e.g. EGFR/Wnt signaling) and morphogenesis in vivo. However, the usefulness of live imaging as a research tool has been severely limited by the need to immobilize worms prior to and during imaging. Conventionally, immobilization is achieved by employing both physical and chemical interventions. These are known to significantly affect many physiological processes, and thus limit our understanding of dynamic developmental processes. Herein we present a novel, easy-to-use microfluidic platform for the long-term immobilization of viable, normally developing C. elegans, compatible with image acquisition at high resolution, thereby overcoming the limitations associated with conventional worm immobilization. The capabilities of the platform are demonstrated through the continuous assessment of anchor cell (AC) invasion and distal tip cell (DTC) migration in larval C. elegans and germ cell apoptosis in adult C. elegans in vivo for the first time.

Effective use of principal component analysis with high resolution remote sensing data to delineate hydrothermal alteration and carbonate rocks

NASA Technical Reports Server (NTRS)

Feldman, Sandra C.

1987-01-01

Methods of applying principal component (PC) analysis to high resolution remote sensing imagery were examined. Using Airborne Imaging Spectrometer (AIS) data, PC analysis was found to be useful for removing the effects of albedo and noise and for isolating the significant information on argillic alteration, zeolite, and carbonate minerals. An effective technique for using PC analysis using an input the first 16 AIS bands, 7 intermediate bands, and the last 16 AIS bands from the 32 flat field corrected bands between 2048 and 2337 nm. Most of the significant mineralogical information resided in the second PC. PC color composites and density sliced images provided a good mineralogical separation when applied to a AIS data set. Although computer intensive, the advantage of PC analysis is that it employs algorithms which already exist on most image processing systems.

Imaging plasmodesmata with high-resolution scanning electron microscopy.

PubMed

Barton, Deborah A; Overall, Robyn L

2015-01-01

High-resolution scanning electron microscopy (HRSEM) is an effective tool to investigate the distribution of plasmodesmata within plant cell walls as well as to probe their complex, three-dimensional architecture. It is a useful alternative to traditional transmission electron microscopy (TEM) in which plasmodesmata are sectioned to reveal their internal substructures. Benefits of adopting an HRSEM approach to studies of plasmodesmata are that the specimen preparation methods are less complex and time consuming than for TEM, many plasmodesmata within a large region of tissue can be imaged in a single session, and three-dimensional information is readily available without the need for reconstructing TEM serial sections or employing transmission electron tomography, both of which are lengthy processes. Here we describe methods to prepare plant samples for HRSEM using pre- or postfixation extraction of cellular material in order to visualize plasmodesmata embedded within plant cell walls.

Gravitational Microlensing Observations of Two New Exoplanets Using the Deep Impact High Resolution Instrument

NASA Astrophysics Data System (ADS)

Barry, Richard K.; Bennett, D. P.; Klaasen, K.; Becker, A. C.; Christiansen, J.; Albrow, M.

2014-01-01

We have worked to characterize two exoplanets newly detected from the ground: OGLE-2012-BLG-0406 and OGLE-2012-BLG-0838, using microlensing observations of the Galactic Bulge recently obtained by NASA’s Deep Impact (DI) spacecraft, in combination with ground data. These observations of the crowded Bulge fields from Earth and from an observatory at a distance of ~1 AU have permitted the extraction of a microlensing parallax signature - critical for breaking exoplanet model degeneracies. For this effort, we used DI’s High Resolution Instrument, launched with a permanent defocus aberration due to an error in cryogenic testing. We show how the effects of a very large, chromatic PSF can be reduced in differencing photometry. We also compare two approaches to differencing photometry - one of which employs the Bramich algorithm and another using the Fruchter & Hook drizzle algorithm.

Ranging performance of satellite laser altimeters

NASA Technical Reports Server (NTRS)

Gardner, Chester S.

1992-01-01

Topographic mapping of the earth, moon and planets can be accomplished with high resolution and accuracy using satellite laser altimeters. These systems employ nanosecond laser pulses and microradian beam divergences to achieve submeter vertical range resolution from orbital altitudes of several hundred kilometers. Here, we develop detailed expressions for the range and pulse width measurement accuracies and use the results to evaluate the ranging performances of several satellite laser altimeters currently under development by NASA for launch during the next decade. Our analysis includes the effects of the target surface characteristics, spacecraft pointing jitter and waveform digitizer characteristics. The results show that ranging accuracy is critically dependent on the pointing accuracy and stability of the altimeter especially over high relief terrain where surface slopes are large. At typical orbital altitudes of several hundred kilometers, single-shot accuracies of a few centimeters can be achieved only when the pointing jitter is on the order of 10 mu rad or less.

SQCRAMscope imaging of transport in an iron-pnictide superconductor

NASA Astrophysics Data System (ADS)

Yang, Fan; Kollar, Alicia; Taylor, Stephen; Palmstrom, Johanna; Chu, Jiun-Haw; Fisher, Ian; Lev, Benjamin

2017-04-01

Microscopic imaging of local magnetic fields provides a window into the organizing principles of complex and technologically relevant condensed matter materials. However, a wide variety of intriguing strongly correlated and topologically nontrivial materials exhibit poorly understood phenomena outside the detection capability of state-of-the-art high-sensitivity, high-resolution scanning probe magnetometers. We have recently introduced a quantum-noise-limited scanning probe magnetometer that can operate from room-to-cryogenic temperatures with unprecedented DC-field sensitivity and micron-scale resolution. The Scanning Quantum Cryogenic Atom Microscope (SQCRAMscope) employs a magnetically levitated atomic Bose-Einstein condensate (BEC), thereby providing immunity to conductive and blackbody radiative heating. We will report on the first use of the SQCRAMscope for imaging a strongly correlated material. Specifically, we will present measurements of electron transport in iron-pnictide superconductors across the electron nematic phase transition at T = 135 K.

Charles Hard Townes: Remarkable Scientist and Inspiring Teacher

NASA Astrophysics Data System (ADS)

Goldsmith, P. F.

2015-05-01

Charles Townes is renowned for his work elucidating the structure of molecules through microwave spectroscopy and for his invention of the maser and the laser. He also had a lifelong interest in astronomy, and in the later portion of his remarkable and long career devoted himself to astronomical research, pioneering the study of molecules in interstellar space and the development of infrared spectroscopy, first from the ground and then from airborne facilities. His interest in high angular resolution, as well as high spectral resolution observations, led to development of the first infrared spatial interferometer employing coherent signal processing techniques. In this short review I will only touch on some of Townes' many scientific contributions, concentrating on astronomy, and will also give some personal thoughts about how he inspired students in their research, helping to make the "Townes Group" at the University of California, Berkeley, an ideal environment in which to start a career in research.

Algorithms and methodology used in constructing high-resolution terrain databases

NASA Astrophysics Data System (ADS)

Williams, Bryan L.; Wilkosz, Aaron

1998-07-01

This paper presents a top-level description of methods used to generate high-resolution 3D IR digital terrain databases using soft photogrammetry. The 3D IR database is derived from aerial photography and is made up of digital ground plane elevation map, vegetation height elevation map, material classification map, object data (tanks, buildings, etc.), and temperature radiance map. Steps required to generate some of these elements are outlined. The use of metric photogrammetry is discussed in the context of elevation map development; and methods employed to generate the material classification maps are given. The developed databases are used by the US Army Aviation and Missile Command to evaluate the performance of various missile systems. A discussion is also presented on database certification which consists of validation, verification, and accreditation procedures followed to certify that the developed databases give a true representation of the area of interest, and are fully compatible with the targeted digital simulators.

Low Prevalence of Chronic Beryllium Disease Among Workers at aNuclearWeaponsResearchandDevelopmentFacility

PubMed Central

Arjomandi, Mehrdad; Seward, James; Gotway, Michael B.; Nishimura, Stephen; Fulton, George P.; Thundiyil, Josef; King, Talmadge E.; Harber, Philip; Balmes, John R.

2012-01-01

Objective To study the prevalence of beryllium sensitization (BeS) and chronic beryllium disease (CBD) in a cohort of workers from a nuclear weapons research and development facility. Methods We evaluated 50 workers with BeS with medical and occupational histories, physical examination, chest imaging with high-resolution computed tomography (N = 49), and pulmonary function testing. Forty of these workers also underwent bronchoscopy for bronchoalveolar lavage and transbronchial biopsies. Results The mean duration of employment at the facility was 18 years and the mean latency (from first possible exposure) to time of evaluation was 32 years. Five of the workers had CBD at the time of evaluation (based on histology or high-resolution computed tomography); three others had evidence of probable CBD. Conclusions These workers with BeS, characterized by a long duration of potential Be exposure and a long latency, had a low prevalence of CBD. PMID:20523233

Characterization of surface properties over permafrost soils using a high resolution mid-infrared camera as part of the Carbon in the Arctic Vulnerability Experiment (CARVE)

NASA Astrophysics Data System (ADS)

Steiner, N.; McDonald, K. C.; Podest, E.; Dinardo, S. J.; Miller, C. E.

2016-12-01

Freeze/thaw and hydrologic cycling have important influence over surface processes in Arctic ecosystems and in Arctic carbon cycling. The seasonal freezing and thawing of soils bracket negative and positive modes of CO2 and CH4 flux of the bulk landscape. Hydrologic processes, such as seasonal inundation of thawed tundra create a complex microtopography where greenhouse-gas sources and sinks occur over short distances. Because of a high spatial variability hydrologic features must be mapped at fine resolution. These mappings can then be compared to local and regional scale observations of surface conditions, such as temperature and freeze/thaw state, to create better estimates of these important surface fields. The Carbon in the Arctic Vulnerability Experiment (CARVE) monitors carbon gas cycling in Alaskan using aircraft-deployed gas sampling instruments along with remote sensing observations of the land surface condition. A nadir-pointed, forward looking infrared (FLIR) imager mounted on the CARVE air-craft is used to measure upwelling mid-infrared spectral radiance at 3-5 microns. The FLIR instrument was operated during the spring, summer and fall seasons, 2013 through 2015. The instantaneous field of view (IFOV) of the FLIR instrument allows for a sub-meter resolution from a height of 500 m. High resolution data products allows for the discrimination of individual landscape components such as soil, vegetation and surface water features in the image footprint. We assess the effectiveness of the FLIR thermal images in monitoring thawing and inundation processes at very high resolutions. Analyses of FLIR datasets over focused study areas emphasizing exploration of the FLIR dataset utility for detailed land surface characterization as related to surface moisture and temperature. Emphasis is given to the Barrow CMDL station site and employ the tram-based data collections there. We will also examine potential at other high latitude sites of interest, e.g. Atqasuk, Ivotuk Alaska and tundra polygon sites under study by collaborators at UT Austin. The combination of high resolution temperature observations with associated estimates of temperature from other instruments can be used to discriminate hydrologic from temperature features in the mid-infrared to produce a high-resolution hydrology product.

Rapid identif ication and comparative analysis of chemical constituents in herbal medicine Fufang decoction by ultra-high-pressure liquid chromatography coupled with a hybrid linear ion trap-high-resolution mass spectrometry.

PubMed

Cao, Gang; Chen, Xiaocheng; Wu, Xin; Li, Qinglin; Zhang, Hongyan

2015-05-01

This study was conducted to reveal the relation between herbal medicine Fufang decoction and a single drug in terms of material base. Da-Cheng-Qi decoction (DCQD) was used as a model. Ultrahigh-pressure liquid chromatography coupled with a hybrid linear ion trap-high-resolution mass spectrometry (UHPLC-LTQ-Orbitrap) was applied to detect and identify the main chemical compounds. This technique was also employed to determine the different chemical components. Under optimized liquid chromatography and mass spectrometry conditions, 64 components, including iridoids, flavonoids, anthraquinones and coumarins, were separated and tentatively characterized in Da-Cheng-Qi decoction. After decoction, the contents of 18 compounds were markedly changed, and two components were no longer detected in Fufang decoction compared with single-medicine decoction. The established method provided a good example for the rapid identification of complicated polar constituents in herbal medicine prescriptions. Copyright © 2014 John Wiley & Sons, Ltd.

High resolution mass approach to characterize refrigerated black truffles stored under different storage atmospheres.

PubMed

Longo, Edoardo; Morozova, Ksenia; Loizzo, Monica R; Tundis, Rosa; Savini, Sara; Foligni, Roberta; Mozzon, Massimo; Martin-Vertedor, Daniel; Scampicchio, Matteo; Boselli, Emanuele

2017-12-01

Freshly harvested Tuber melanosporum samples were packed and stored at 4°C under reduced atmospheric pressure or modified atmosphere for four weeks. Multivariate analysis was employed to correlate the antioxidant power of the ethanolic extracts of the samples with the chemical composition determined by high resolution mass spectrometry. High performance liquid chromatography coupled with a coularray detector was applied to select the chemical species associated with the antioxidant power. Four classes of chemical compounds were investigated in more detail by a targeted approach: derivatives of glutathione, adenine (such as S-adenosyl-homocysteine), oxidized linoleic acid and ergosterol. Adducts containing glutathione and adenine with oxidized linoleic acid were observed in TM for the first time and can be considered markers of freshness of the product. S-adenosyl-homocysteine, the acetyl-carnitine adduct with cysteinyl-glycine and several oxidized linoleic acid derivatives were among the markers of degradation. Copyright © 2017. Published by Elsevier Ltd.

Real-time dual-comb spectroscopy with a free-running bidirectionally mode-locked fiber laser

NASA Astrophysics Data System (ADS)

Mehravar, S.; Norwood, R. A.; Peyghambarian, N.; Kieu, K.

2016-06-01

Dual-comb technique has enabled exciting applications in high resolution spectroscopy, precision distance measurements, and 3D imaging. Major advantages over traditional methods can be achieved with dual-comb technique. For example, dual-comb spectroscopy provides orders of magnitude improvement in acquisition speed over standard Fourier-transform spectroscopy while still preserving the high resolution capability. Wider adoption of the technique has, however, been hindered by the need for complex and expensive ultrafast laser systems. Here, we present a simple and robust dual-comb system that employs a free-running bidirectionally mode-locked fiber laser operating at telecommunication wavelength. Two femtosecond frequency combs (with a small difference in repetition rates) are generated from a single laser cavity to ensure mutual coherent properties and common noise cancellation. As the result, we have achieved real-time absorption spectroscopy measurements without the need for complex servo locking with accurate frequency referencing, and relatively high signal-to-noise ratio.

A high-resolution 7-Tesla fMRI dataset from complex natural stimulation with an audio movie.

PubMed

Hanke, Michael; Baumgartner, Florian J; Ibe, Pierre; Kaule, Falko R; Pollmann, Stefan; Speck, Oliver; Zinke, Wolf; Stadler, Jörg

2014-01-01

Here we present a high-resolution functional magnetic resonance (fMRI) dataset - 20 participants recorded at high field strength (7 Tesla) during prolonged stimulation with an auditory feature film ("Forrest Gump"). In addition, a comprehensive set of auxiliary data (T1w, T2w, DTI, susceptibility-weighted image, angiography) as well as measurements to assess technical and physiological noise components have been acquired. An initial analysis confirms that these data can be used to study common and idiosyncratic brain response patterns to complex auditory stimulation. Among the potential uses of this dataset are the study of auditory attention and cognition, language and music perception, and social perception. The auxiliary measurements enable a large variety of additional analysis strategies that relate functional response patterns to structural properties of the brain. Alongside the acquired data, we provide source code and detailed information on all employed procedures - from stimulus creation to data analysis. In order to facilitate replicative and derived works, only free and open-source software was utilized.

Terahertz quantum-cascade lasers as high-power and wideband, gapless sources for spectroscopy.

PubMed

Röben, Benjamin; Lü, Xiang; Hempel, Martin; Biermann, Klaus; Schrottke, Lutz; Grahn, Holger T

2017-07-10

Terahertz (THz) quantum-cascade lasers (QCLs) are powerful radiation sources for high-resolution and high-sensitivity spectroscopy with a discrete spectrum between 2 and 5 THz as well as a continuous coverage of several GHz. However, for many applications, a radiation source with a continuous coverage of a substantially larger frequency range is required. We employed a multi-mode THz QCL operated with a fast ramped injection current, which leads to a collective tuning of equally-spaced Fabry-Pérot laser modes exceeding their separation. A continuous coverage over 72 GHz at about 4.7 THz was achieved. We demonstrate that the QCL is superior to conventional sources used in Fourier transform infrared spectroscopy in terms of the signal-to-noise ratio as well as the dynamic range by one to two orders of magnitude. Our results pave the way for versatile THz spectroscopic systems with unprecedented resolution and sensitivity across a wide frequency range.

Use of high-granularity CdZnTe pixelated detectors to correct response non-uniformities caused by defects in crystals

DOE PAGES

Bolotnikov, A. E.; Camarda, G. S.; Cui, Y.; ...

2015-09-06

Following our successful demonstration of the position-sensitive virtual Frisch-grid detectors, we investigated the feasibility of using high-granularity position sensing to correct response non-uniformities caused by the crystal defects in CdZnTe (CZT) pixelated detectors. The development of high-granularity detectors able to correct response non-uniformities on a scale comparable to the size of electron clouds opens the opportunity of using unselected off-the-shelf CZT material, whilst still assuring high spectral resolution for the majority of the detectors fabricated from an ingot. Here, we present the results from testing 3D position-sensitive 15×15×10 mm 3 pixelated detectors, fabricated with conventional pixel patterns with progressively smallermore » pixel sizes: 1.4, 0.8, and 0.5 mm. We employed the readout system based on the H3D front-end multi-channel ASIC developed by BNL's Instrumentation Division in collaboration with the University of Michigan. We use the sharing of electron clouds among several adjacent pixels to measure locations of interaction points with sub-pixel resolution. By using the detectors with small-pixel sizes and a high probability of the charge-sharing events, we were able to improve their spectral resolutions in comparison to the baseline levels, measured for the 1.4-mm pixel size detectors with small fractions of charge-sharing events. These results demonstrate that further enhancement of the performance of CZT pixelated detectors and reduction of costs are possible by using high spatial-resolution position information of interaction points to correct the small-scale response non-uniformities caused by crystal defects present in most devices.« less

Lunar Observer Laser Altimeter observations for lunar base site selection

NASA Technical Reports Server (NTRS)

Garvin, James B.; Bufton, Jack L.

1992-01-01

One of the critical datasets for optimal selection of future lunar landing sites is local- to regional-scale topography. Lunar base site selection will require such data for both engineering and scientific operations purposes. The Lunar Geoscience Orbiter or Lunar Observer is the ideal precursory science mission from which to obtain this required information. We suggest that a simple laser altimeter instrument could be employed to measure local-scale slopes, heights, and depths of lunar surface features important to lunar base planning and design. For this reason, we have designed and are currently constructing a breadboard of a Lunar Observer Laser Altimeter (LOLA) instrument capable of acquiring contiguous-footprint topographic profiles with both 30-m and 300-m along-track resolution. This instrument meets all the severe weight, power, size, and data rate limitations imposed by Observer-class spacecraft. In addition, LOLA would be capable of measuring the within-footprint vertical roughness of the lunar surface, and the 1.06-micron relative surface reflectivity at normal incidence. We have used airborne laser altimeter data for a few representative lunar analog landforms to simulate and analyze LOLA performance in a 100-km lunar orbit. We demonstrate that this system in its highest resolution mode (30-m diameter footprints) would quantify the topography of all but the very smallest lunar landforms. At its global mapping resolution (300-m diameter footprints), LOLA would establish the topographic context for lunar landing site selection by providing the basis for constructing a 1-2 km spatial resolution global, geodetic topographic grid that would contain a high density of observations (e.g., approximately 1000 observations per each 1 deg by 1 deg cell at the lunar equator). The high spatial and vertical resolution measurements made with a LOLA-class instrument on a precursory Lunar Observer would be highly synergistic with high-resolution imaging datasets, and will allow for direct quantification of critical slopes, heights, and depths of features visible in images of potential lunar base sites.

Mutual information registration of multi-spectral and multi-resolution images of DigitalGlobe's WorldView-3 imaging satellite

NASA Astrophysics Data System (ADS)

Miecznik, Grzegorz; Shafer, Jeff; Baugh, William M.; Bader, Brett; Karspeck, Milan; Pacifici, Fabio

2017-05-01

WorldView-3 (WV-3) is a DigitalGlobe commercial, high resolution, push-broom imaging satellite with three instruments: visible and near-infrared VNIR consisting of panchromatic (0.3m nadir GSD) plus multi-spectral (1.2m), short-wave infrared SWIR (3.7m), and multi-spectral CAVIS (30m). Nine VNIR bands, which are on one instrument, are nearly perfectly registered to each other, whereas eight SWIR bands, belonging to the second instrument, are misaligned with respect to VNIR and to each other. Geometric calibration and ortho-rectification results in a VNIR/SWIR alignment which is accurate to approximately 0.75 SWIR pixel at 3.7m GSD, whereas inter-SWIR, band to band registration is 0.3 SWIR pixel. Numerous high resolution, spectral applications, such as object classification and material identification, require more accurate registration, which can be achieved by utilizing image processing algorithms, for example Mutual Information (MI). Although MI-based co-registration algorithms are highly accurate, implementation details for automated processing can be challenging. One particular challenge is how to compute bin widths of intensity histograms, which are fundamental building blocks of MI. We solve this problem by making the bin widths proportional to instrument shot noise. Next, we show how to take advantage of multiple VNIR bands, and improve registration sensitivity to image alignment. To meet this goal, we employ Canonical Correlation Analysis, which maximizes VNIR/SWIR correlation through an optimal linear combination of VNIR bands. Finally we explore how to register images corresponding to different spatial resolutions. We show that MI computed at a low-resolution grid is more sensitive to alignment parameters than MI computed at a high-resolution grid. The proposed modifications allow us to improve VNIR/SWIR registration to better than ¼ of a SWIR pixel, as long as terrain elevation is properly accounted for, and clouds and water are masked out.

An iterative reconstruction method for high-pitch helical luggage CT

NASA Astrophysics Data System (ADS)

Xue, Hui; Zhang, Li; Chen, Zhiqiang; Jin, Xin

2012-10-01

X-ray luggage CT is widely used in airports and railway stations for the purpose of detecting contrabands and dangerous goods that may be potential threaten to public safety, playing an important role in homeland security. An X-ray luggage CT is usually in a helical trajectory with a high pitch for achieving a high passing speed of the luggage. The disadvantage of high pitch is that conventional filtered back-projection (FBP) requires a very large slice thickness, leading to bad axial resolution and helical artifacts. Especially when severe data inconsistencies are present in the z-direction, like the ends of a scanning object, the partial volume effect leads to inaccuracy value and may cause a wrong identification. In this paper, an iterative reconstruction method is developed to improve the image quality and accuracy for a large-spacing multi-detector high-pitch helical luggage CT system. In this method, the slice thickness is set to be much smaller than the pitch. Each slice involves projection data collected in a rather small angular range, being an ill-conditioned limited-angle problem. Firstly a low-resolution reconstruction is employed to obtain images, which are used as prior images in the following process. Then iterative reconstruction is performed to obtain high-resolution images. This method enables a high volume coverage speed and a thin reconstruction slice for the helical luggage CT. We validate this method with data collected in a commercial X-ray luggage CT.

Tutorial on photoacoustic tomography

PubMed Central

Zhou, Yong; Yao, Junjie; Wang, Lihong V.

2016-01-01

Abstract. Photoacoustic tomography (PAT) has become one of the fastest growing fields in biomedical optics. Unlike pure optical imaging, such as confocal microscopy and two-photon microscopy, PAT employs acoustic detection to image optical absorption contrast with high-resolution deep into scattering tissue. So far, PAT has been widely used for multiscale anatomical, functional, and molecular imaging of biological tissues. We focus on PAT’s basic principles, major implementations, imaging contrasts, and recent applications. PMID:27086868

Enhancing Deep-Water Low-Resolution Gridded Bathymetry Using Single Image Super-Resolution

NASA Astrophysics Data System (ADS)

Elmore, P. A.; Nock, K.; Bonanno, D.; Smith, L.; Ferrini, V. L.; Petry, F. E.

2017-12-01

We present research to employ single-image super-resolution (SISR) algorithms to enhance knowledge of the seafloor using the 1-minute GEBCO 2014 grid when 100m grids from high-resolution sonar systems are available for training. Our numerical upscaling experiments of x15 upscaling of the GEBCO grid along three areas of the Eastern Pacific Ocean along mid-ocean ridge systems where we have these 100m gridded bathymetry data sets, which we accept as ground-truth. We show that four SISR algorithms can enhance this low-resolution knowledge of bathymetry versus bicubic or Spline-In-Tension algorithms through upscaling under these conditions: 1) rough topography is present in both training and testing areas and 2) the range of depths and features in the training area contains the range of depths in the enhancement area. We quantitatively judged successful SISR enhancement versus bicubic interpolation when Student's hypothesis testing show significant improvement of the root-mean squared error (RMSE) between upscaled bathymetry and 100m gridded ground-truth bathymetry at p < 0.05. In addition, we found evidence that random forest based SISR methods may provide more robust enhancements versus non-forest based SISR algorithms.

Detecting phase-amplitude coupling with high frequency resolution using adaptive decompositions

PubMed Central

Pittman-Polletta, Benjamin; Hsieh, Wan-Hsin; Kaur, Satvinder; Lo, Men-Tzung; Hu, Kun

2014-01-01

Background Phase-amplitude coupling (PAC) – the dependence of the amplitude of one rhythm on the phase of another, lower-frequency rhythm – has recently been used to illuminate cross-frequency coordination in neurophysiological activity. An essential step in measuring PAC is decomposing data to obtain rhythmic components of interest. Current methods of PAC assessment employ narrowband Fourier-based filters, which assume that biological rhythms are stationary, harmonic oscillations. However, biological signals frequently contain irregular and nonstationary features, which may contaminate rhythms of interest and complicate comodulogram interpretation, especially when frequency resolution is limited by short data segments. New method To better account for nonstationarities while maintaining sharp frequency resolution in PAC measurement, even for short data segments, we introduce a new method of PAC assessment which utilizes adaptive and more generally broadband decomposition techniques – such as the empirical mode decomposition (EMD). To obtain high frequency resolution PAC measurements, our method distributes the PAC associated with pairs of broadband oscillations over frequency space according to the time-local frequencies of these oscillations. Comparison with existing methods We compare our novel adaptive approach to a narrowband comodulogram approach on a variety of simulated signals of short duration, studying systematically how different types of nonstationarities affect these methods, as well as on EEG data. Conclusions Our results show: (1) narrowband filtering can lead to poor PAC frequency resolution, and inaccuracy and false negatives in PAC assessment; (2) our adaptive approach attains better PAC frequency resolution and is more resistant to nonstationarities and artifacts than traditional comodulograms. PMID:24452055

The evaluation of a deformable diffraction grating for a stigmatic EUV spectroheliometer

NASA Technical Reports Server (NTRS)

Timothy, J. G.

1987-01-01

A high-efficiency, extreme ultraviolet (EUV) imaging spectrometer is constructed and tested. The spectrometer employs a concave toroidal grating illuminated at normal incidence in a Rowland circle mounting and has only one reflecting surface. The toroidal grating has been fabricated by a new technique employing an elastically-deformable sub-master grating replicated in a spherical form and then mechanically distorted to produce the desired aspect ratio of the toroidal surface for stigmatic imaging over the selected wavelength range. The fixed toroidal grating used in the spectrometer is then replicated from this surface. Photographic tests and initial photoelectric tests with a two-dimensional, pulse-counting detector system verify the image quality of the toroidal grating at wavelengths near 600 A. The results of these tests and the basic designs of two instruments which could employ the imaging spectrometer for astrophysical investigations in space are described; i.e., a high-resolution EUV spectroheliometer for studies of the solar chromosphere, transition region, and corona; and an EUV spectroscopic telescope for studies of non-solar objects.

Monitoring of Vegetation Impact Due to Trampling on Cadillac Mountain Summit Using High Spatial Resolution Remote Sensing Data Sets

NASA Astrophysics Data System (ADS)

Kim, Min-Kook; Daigle, John J.

2012-11-01

Cadillac Mountain—the highest peak along the eastern seaboard of the United States—is a major tourist destination in Acadia National Park, Maine. Managing vegetation impact due to trampling on the Cadillac Mountain summit is extremely challenging because of the large number of visitors and the general open nature of landscape in this fragile subalpine environmental setting. Since 2000, more intensive management strategies—based on placing physical barriers and educational messages for visitors—have been employed to protect threatened vegetation, decrease vegetation impact, and enhance vegetation recovery in the vicinity of the summit loop trail. The primary purpose of this study was to evaluate the effect of the management strategies employed. For this purpose, vegetation cover changes between 2001 and 2007 were detected using multispectral high spatial resolution remote sensing data sets. A normalized difference vegetation index was employed to identify the rates of increase and decrease in the vegetation areas. Three buffering distances (30, 60, and 90 m) from the edges of the trail were used to define multiple spatial extents of the site, and the same spatial extents were employed at a nearby control site that had no visitors. No significant differences were detected between the mean rates of vegetation increase and decrease at the experimental site compared with a nearby control site in the case of a small spatial scale (≤30 m) comparison (in all cases P > 0.05). However, in the medium (≤60 m) and large (≤90 m) spatial scales, the rates of increased vegetation were significantly greater and rates of decreased vegetation significantly lower at the experimental site compared with the control site (in all cases P < 0.001). Research implications are explored that relate to the spatial extent of the radial patterns of impact of trampling on vegetation at the site level. Management implications are explored in terms of the spatial strategies used to decrease the impact of trampling on vegetation.

Method to Biomonitor the Cooked Meat Carcinogen 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in Dyed Hair by Ultra-Performance Liquid Chromatography-Orbitrap High Resolution Multistage Mass Spectrometry.

PubMed

Guo, Jingshu; Yonemori, Kim; Le Marchand, Loïc; Turesky, Robert J

2015-06-16

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a carcinogenic heterocyclic aromatic amine formed in cooked meat. The use of naturally colored hair containing PhIP can serve as a long-term biomarker of exposure to this carcinogen. However, the measurement of PhIP in dyed hair, a cosmetic treatment commonly used by the adult population, is challenging because the dye process introduces into the hair matrix a complex mixture of chemicals that interferes with the measurement of PhIP. The high-resolution scanning features of the Orbitrap Fusion mass spectrometer were employed to biomonitor PhIP in dyed hair. Because of the complexity of chemicals in the hair dye, the consecutive reaction monitoring of PhIP at the MS(3) scan stage was employed to selectively remove the isobaric interferences. The limit of quantification (LOQ) of PhIP was 84 parts-per-trillion (ppt) employing 50 mg of hair. Calibration curves were generated in dyed hair matrixes and showed good linearity (40-1000 pg PhIP/g hair) with a goodness-of-fit regression value of r(2) > 0.9978. The within-day (between-day) coefficients of variation were 7.7% (17%) and 5.4% (6.1%), respectively, with dyed hair samples spiked with PhIP at 200 and 600 ppt. The levels of PhIP accrued in dyed hair from volunteers on a semicontrolled feeding study who ingested known levels of PhIP were comparable to the levels of PhIP accrued in hair of subjects with natural hair color. The method was successfully employed to measure PhIP in nondyed and dyed hair biospecimens of participants in a case-control study of colorectal adenoma on their regular diet.

Direct determination of chromium in infant formulas employing high-resolution continuum source electrothermal atomic absorption spectrometry and solid sample analysis.

PubMed

Silva, Arlene S; Brandao, Geovani C; Matos, Geraldo D; Ferreira, Sergio L C

2015-11-01

The present work proposed an analytical method for the direct determination of chromium in infant formulas employing the high-resolution continuum source electrothermal atomic absorption spectrometry combined with the solid sample analysis (SS-HR-CS ET AAS). Sample masses up to 2.0mg were directly weighted on a solid sampling platform and introduced into the graphite tube. In order to minimize the formation of carbonaceous residues and to improve the contact of the modifier solution with the solid sample, a volume of 10 µL of a solution containing 6% (v/v) H2O2, 20% (v/v) ethanol and 1% (v/v) HNO3 was added. The pyrolysis and atomization temperatures established were 1600 and 2400 °C, respectively, using magnesium as chemical modifier. The calibration technique was evaluated by comparing the slopes of calibration curves established using aqueous and solid standards. This test revealed that chromium can be determined employing the external calibration technique using aqueous standards. Under these conditions, the method developed allows the direct determination of chromium with limit of quantification of 11.5 ng g(-1), precision expressed as relative standard deviation (RSD) in the range of 4.0-17.9% (n=3) and a characteristic mass of 1.2 pg of chromium. The accuracy was confirmed by analysis of a certified reference material of tomato leaves furnished by National Institute of Standards and Technology. The method proposed was applied for the determination of chromium in five different infant formula samples. The chromium content found varied in the range of 33.9-58.1 ng g(-1) (n=3). These samples were also analyzed employing ICP-MS. A statistical test demonstrated that there is no significant difference between the results found by two methods. The chromium concentrations achieved are lower than the maximum limit permissible for chromium in foods by Brazilian Legislation. Copyright © 2015. Published by Elsevier B.V.

A Method to Biomonitor the Cooked Meat Carcinogen 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in Dyed Hair by Ultra Performance Liquid Chromatography-Orbitrap High Resolution Multistage Mass Spectrometry

PubMed Central

Guo, Jingshu; Yonemori, Kim; Le Marchand, Loïc; Turesky, Robert J.

2015-01-01

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a carcinogenic heterocyclic aromatic amine formed in cooked meat. The use of naturally colored hair containing PhIP can serve as a long-term biomarker of exposure to this carcinogen. However, the measurement of PhIP in dyed hair, a cosmetic treatment commonly used by the adult population, is challenging because the dye process introduces a complex mixture of chemicals into the hair matrix, which interfere with the measurement of PhIP. The high-resolution scanning features of the Orbitrap Fusion™ mass spectrometer were employed to biomonitor PhIP in dyed hair. Because of the complexity of chemicals in the hair dye, the consecutive reaction monitoring of PhIP at the MS3 scan stage was employed to selectively remove the isobaric interferences. The limit of quantification (LOQ) of PhIP was 84 parts-per-trillion (ppt) employing 50 mg hair. Calibration curves were generated in dyed hair matrices and showed good linearity (40 to 1000 pg PhIP/g hair) with a goodness-of-fit regression value r2 > 0.9978. The within-day (between-day) coefficients of variation were 7.7% (17%) and 5.4% (6.1%), respectively, with dyed hair samples spiked with PhIP at 200 and 600 ppt. The levels of PhIP accrued in dyed hair from volunteers on a semi-controlled feeding study who ingested known levels of PhIP were comparable to the levels of PhIP accrued in hair of subjects with natural hair color. The method was successfully employed to measure PhIP in non-dyed and dyed hair biospecimens of participants in a case-control study of colorectal adenoma on their regular diet. PMID:25969997

Monitoring of vegetation impact due to trampling on Cadillac Mountain summit using high spatial resolution remote sensing data sets.

PubMed

Kim, Min-Kook; Daigle, John J

2012-11-01

Cadillac Mountain--the highest peak along the eastern seaboard of the United States--is a major tourist destination in Acadia National Park, Maine. Managing vegetation impact due to trampling on the Cadillac Mountain summit is extremely challenging because of the large number of visitors and the general open nature of landscape in this fragile subalpine environmental setting. Since 2000, more intensive management strategies--based on placing physical barriers and educational messages for visitors--have been employed to protect threatened vegetation, decrease vegetation impact, and enhance vegetation recovery in the vicinity of the summit loop trail. The primary purpose of this study was to evaluate the effect of the management strategies employed. For this purpose, vegetation cover changes between 2001 and 2007 were detected using multispectral high spatial resolution remote sensing data sets. A normalized difference vegetation index was employed to identify the rates of increase and decrease in the vegetation areas. Three buffering distances (30, 60, and 90 m) from the edges of the trail were used to define multiple spatial extents of the site, and the same spatial extents were employed at a nearby control site that had no visitors. No significant differences were detected between the mean rates of vegetation increase and decrease at the experimental site compared with a nearby control site in the case of a small spatial scale (≤30 m) comparison (in all cases P > 0.05). However, in the medium (≤60 m) and large (≤90 m) spatial scales, the rates of increased vegetation were significantly greater and rates of decreased vegetation significantly lower at the experimental site compared with the control site (in all cases P < 0.001). Research implications are explored that relate to the spatial extent of the radial patterns of impact of trampling on vegetation at the site level. Management implications are explored in terms of the spatial strategies used to decrease the impact of trampling on vegetation.

Fusion of MultiSpectral and Panchromatic Images Based on Morphological Operators.

PubMed

Restaino, Rocco; Vivone, Gemine; Dalla Mura, Mauro; Chanussot, Jocelyn

2016-04-20

Nonlinear decomposition schemes constitute an alternative to classical approaches for facing the problem of data fusion. In this paper we discuss the application of this methodology to a popular remote sensing application called pansharpening, which consists in the fusion of a low resolution multispectral image and a high resolution panchromatic image. We design a complete pansharpening scheme based on the use of morphological half gradients operators and demonstrate the suitability of this algorithm through the comparison with state of the art approaches. Four datasets acquired by the Pleiades, Worldview-2, Ikonos and Geoeye-1 satellites are employed for the performance assessment, testifying the effectiveness of the proposed approach in producing top-class images with a setting independent of the specific sensor.

A broad look at solar physics adapted from the solar physics study of August 1975

NASA Technical Reports Server (NTRS)

Parker, E.; Timothy, A.; Beckers, J.; Hundhausen, A.; Kundu, M. R.; Leith, C. E.; Lin, R.; Linsky, J.; Macdonald, F. B.; Noyes, R.

1979-01-01

The current status of our knowledge of the basic mechanisms involved in fundamental solar phenomena is reviewed. These include mechanisms responsible for heating the corona, the generation of the solar wind, the particle acceleration in flares, and the dissipation of magnetic energy in field reversal regions, known as current sheets. The discussion covers solar flares and high-energy phenomena, solar active regions; solar interior, convection, and activity; the structure and energetics of the quiet solar atmosphere; the structure of the corona; the solar composition; and solar terrestrial interactions. It also covers a program of solar research, including the special observational requirements for spectral and angular resolution, sensitivity, time resolution, and duration of the techniques employed.

System implications of large radiometric array antennas

NASA Technical Reports Server (NTRS)

Levis, C. A.; Lin, H. C.

1976-01-01

Current radiometric earth and atmospheric sensing systems in the centimeter wavelength range generally employ a directive antenna connected through a single terminal pair to a Dicke receiver. It is shown that this approach does not lend itself to systems with greatly increased spatial resolution. Signal to noise considerations relating to antenna efficiency force the introduction of active elements at the subarray level; thus, if Dicke switching is to be used, it must be distributed throughout the system. Some possible approaches are suggested. The introduction of active elements at the subarray level is found to ease the design constraints on time delay elements, necessary for bandwidth, and on multiple beam generation, required in order to achieve sufficient integration time with high resolution.

Timing Analysis with INTEGRAL: Comparing Different Reconstruction Algorithms

NASA Technical Reports Server (NTRS)

Grinberg, V.; Kreykenboehm, I.; Fuerst, F.; Wilms, J.; Pottschmidt, K.; Bel, M. Cadolle; Rodriquez, J.; Marcu, D. M.; Suchy, S.; Markowitz, A.;

A 2D eye gaze estimation system with low-resolution webcam images

NASA Astrophysics Data System (ADS)

Ince, Ibrahim Furkan; Kim, Jin Woo

2011-12-01

In this article, a low-cost system for 2D eye gaze estimation with low-resolution webcam images is presented. Two algorithms are proposed for this purpose, one for the eye-ball detection with stable approximate pupil-center and the other one for the eye movements' direction detection. Eyeball is detected using deformable angular integral search by minimum intensity (DAISMI) algorithm. Deformable template-based 2D gaze estimation (DTBGE) algorithm is employed as a noise filter for deciding the stable movement decisions. While DTBGE employs binary images, DAISMI employs gray-scale images. Right and left eye estimates are evaluated separately. DAISMI finds the stable approximate pupil-center location by calculating the mass-center of eyeball border vertices to be employed for initial deformable template alignment. DTBGE starts running with initial alignment and updates the template alignment with resulting eye movements and eyeball size frame by frame. The horizontal and vertical deviation of eye movements through eyeball size is considered as if it is directly proportional with the deviation of cursor movements in a certain screen size and resolution. The core advantage of the system is that it does not employ the real pupil-center as a reference point for gaze estimation which is more reliable against corneal reflection. Visual angle accuracy is used for the evaluation and benchmarking of the system. Effectiveness of the proposed system is presented and experimental results are shown.

Workplace discrimination and autism spectrum disorders: The National EEOC Americans with Disabilities Act Research project.

PubMed

Van Wieren, Todd A; Reid, Christine A; McMahon, Brian T

2008-01-01

Using the Integrated Mission System of the Equal Employment Opportunity Commission (EEOC), the employment discrimination experience of Americans with autism spectrum disorders (ASDs) is documented for Title I of the Americans with Disabilities Act. The researchers examine demographic characteristics of the charging parties; the industry designation, location, and size of employers against whom complaints are filed; the nature of discrimination (i.e., type of complaint) alleged to occur; and the legal outcome or resolution of these complaints. Researchers compare and contrast these key dimensions of workplace discrimination involving individuals with ASDs and persons with other physical, sensory, and neurological impairments. Researchers also attempt to discern whether or not the resolutions of the ASD charges can be predicted using the variables available for analysis. The comparative findings of this study indicate that individuals with ASDs were more likely to make charges of discrimination against Retail industry employers. Persons with ASDs were also more likely to make charges of discrimination when they were younger, male, and/or of Native American/Alaskan Native ethnicity. The predictive findings of this study indicate that the odds of ASD charges resulting in meritorious resolution (i.e., discrimination determined by the EEOC to have occurred) increase when the discrimination was encountered in Service industries and by larger employers. Implications for policy, advocacy and further research efforts are addressed.

A high resolution small animal radiation research platform (SARRP) with x-ray tomographic guidance capabilities

PubMed Central

Wong, John; Armour, Elwood; Kazanzides, Peter; Iordachita, Iulian; Tryggestad, Erik; Deng, Hua; Matinfar, Mohammad; Kennedy, Christopher; Liu, Zejian; Chan, Timothy; Gray, Owen; Verhaegen, Frank; McNutt, Todd; Ford, Eric; DeWeese, Theodore L.

2008-01-01

Purpose To demonstrate the CT imaging, conformal irradiation and treatment planning capabilities of a small animal radiation research platform (SARRP). Methods The SARRP employs a dual-focal spot, constant voltage x-ray source mounted on a gantry with a source-to-isocenter distance of 35 cm. Gantry rotation is limited to 120° from vertical. Eighty to 100 kVp x-rays from the smaller 0.4 mm focal spot are used for imaging. Both 0.4 mm and 3.0 mm focal spots operate at 225 kVp for irradiation. Robotic translate/rotate stages are used to position the animal. Cone-beam (CB) CT imaging is achieved by rotating the horizontal animal between the stationary x-ray source and a flat-panel detector. Radiation beams range from 0.5 mm in diameter to (60 × 60) mm2. Dosimetry is measured with radio-chromic films. Monte Carlo dose calculations are employed for treatment planning. The combination of gantry and robotic stage motions facilitate conformal irradiation. Results The SARRP spans 3 ft × 4 ft × 6 ft (WxLxH). Depending on filtration, the isocenter dose outputs at 1 cm depth in water range from 22 to 375 cGy/min from the smallest to the largest radiation fields. The 20% to 80% dose fall-off spans 0.16 mm. CBCT with (0.6 × 0.6 × 0.6) mm3 voxel resolution is acquired with less than 1 cGy. Treatment planning is performed at sub-mm resolution. Conclusions The capability of the SARRP to deliver highly focal beams to multiple animal model systems provides new research opportunities that more realistically bridge laboratory research and clinical translation. PMID:18640502

Workplace discrimination and disfigurement: the national EEOC ADA research project.

PubMed

Tartaglia, Alexander; McMahon, Brian T; West, Steven L; Belongia, Lisa

2005-01-01

Using the Integrated Mission System of the Equal Employment Opportunity Commission, the employment discrimination experience of Americans with disfigurement is documented. Key dimensions of workplace discrimination involving Americans with disfigurement and persons with missing limbs are compared and contrasted. Specifically, the researchers examine demographic characteristics of Charging Parties; the industry designation, location and size of Respondents/employers; the discrimination Issue (i.e., type of adverse action) alleged to occur; and the legal outcome or Resolution of these allegations. Charging Parties with disfigurement who are female or between 30 and 39 years of age are more likely to encounter employment discrimination than their counterparts with missing limbs. Harassment and Non-wage Benefits are the Issues that emerge in higher proportion. Allegations derived from persons with disfigurement are more common in among mid-size employers, those located in the South, or those in Retail or Service industries. Following investigation, allegations derived from persons with disfigurement are less likely to have Merit Resolutions than those brought by Charging Parties with missing limbs.

Optimizing UV Index determination from broadband irradiances

NASA Astrophysics Data System (ADS)

Tereszchuk, Keith A.; Rochon, Yves J.; McLinden, Chris A.; Vaillancourt, Paul A.

2018-03-01

A study was undertaken to improve upon the prognosticative capability of Environment and Climate Change Canada's (ECCC) UV Index forecast model. An aspect of that work, and the topic of this communication, was to investigate the use of the four UV broadband surface irradiance fields generated by ECCC's Global Environmental Multiscale (GEM) numerical prediction model to determine the UV Index. The basis of the investigation involves the creation of a suite of routines which employ high-spectral-resolution radiative transfer code developed to calculate UV Index fields from GEM forecasts. These routines employ a modified version of the Cloud-J v7.4 radiative transfer model, which integrates GEM output to produce high-spectral-resolution surface irradiance fields. The output generated using the high-resolution radiative transfer code served to verify and calibrate GEM broadband surface irradiances under clear-sky conditions and their use in providing the UV Index. A subsequent comparison of irradiances and UV Index under cloudy conditions was also performed. Linear correlation agreement of surface irradiances from the two models for each of the two higher UV bands covering 310.70-330.0 and 330.03-400.00 nm is typically greater than 95 % for clear-sky conditions with associated root-mean-square relative errors of 6.4 and 4.0 %. However, underestimations of clear-sky GEM irradiances were found on the order of ˜ 30-50 % for the 294.12-310.70 nm band and by a factor of ˜ 30 for the 280.11-294.12 nm band. This underestimation can be significant for UV Index determination but would not impact weather forecasting. Corresponding empirical adjustments were applied to the broadband irradiances now giving a correlation coefficient of unity. From these, a least-squares fitting was derived for the calculation of the UV Index. The resultant differences in UV indices from the high-spectral-resolution irradiances and the resultant GEM broadband irradiances are typically within 0.2-0.3 with a root-mean-square relative error in the scatter of ˜ 6.6 % for clear-sky conditions. Similar results are reproduced under cloudy conditions with light to moderate clouds, with a relative error comparable to the clear-sky counterpart; under strong attenuation due to clouds, a substantial increase in the root-mean-square relative error of up to 35 % is observed due to differing cloud radiative transfer models.

An efficient implementation of 3D high-resolution imaging for large-scale seismic data with GPU/CPU heterogeneous parallel computing

NASA Astrophysics Data System (ADS)

Xu, Jincheng; Liu, Wei; Wang, Jin; Liu, Linong; Zhang, Jianfeng

2018-02-01

De-absorption pre-stack time migration (QPSTM) compensates for the absorption and dispersion of seismic waves by introducing an effective Q parameter, thereby making it an effective tool for 3D, high-resolution imaging of seismic data. Although the optimal aperture obtained via stationary-phase migration reduces the computational cost of 3D QPSTM and yields 3D stationary-phase QPSTM, the associated computational efficiency is still the main problem in the processing of 3D, high-resolution images for real large-scale seismic data. In the current paper, we proposed a division method for large-scale, 3D seismic data to optimize the performance of stationary-phase QPSTM on clusters of graphics processing units (GPU). Then, we designed an imaging point parallel strategy to achieve an optimal parallel computing performance. Afterward, we adopted an asynchronous double buffering scheme for multi-stream to perform the GPU/CPU parallel computing. Moreover, several key optimization strategies of computation and storage based on the compute unified device architecture (CUDA) were adopted to accelerate the 3D stationary-phase QPSTM algorithm. Compared with the initial GPU code, the implementation of the key optimization steps, including thread optimization, shared memory optimization, register optimization and special function units (SFU), greatly improved the efficiency. A numerical example employing real large-scale, 3D seismic data showed that our scheme is nearly 80 times faster than the CPU-QPSTM algorithm. Our GPU/CPU heterogeneous parallel computing framework significant reduces the computational cost and facilitates 3D high-resolution imaging for large-scale seismic data.

Fast generation of computer-generated hologram by graphics processing unit

NASA Astrophysics Data System (ADS)

Matsuda, Sho; Fujii, Tomohiko; Yamaguchi, Takeshi; Yoshikawa, Hiroshi

2009-02-01

A cylindrical hologram is well known to be viewable in 360 deg. This hologram depends high pixel resolution.Therefore, Computer-Generated Cylindrical Hologram (CGCH) requires huge calculation amount.In our previous research, we used look-up table method for fast calculation with Intel Pentium4 2.8 GHz.It took 480 hours to calculate high resolution CGCH (504,000 x 63,000 pixels and the average number of object points are 27,000).To improve quality of CGCH reconstructed image, fringe pattern requires higher spatial frequency and resolution.Therefore, to increase the calculation speed, we have to change the calculation method. In this paper, to reduce the calculation time of CGCH (912,000 x 108,000 pixels), we employ Graphics Processing Unit (GPU).It took 4,406 hours to calculate high resolution CGCH on Xeon 3.4 GHz.Since GPU has many streaming processors and a parallel processing structure, GPU works as the high performance parallel processor.In addition, GPU gives max performance to 2 dimensional data and streaming data.Recently, GPU can be utilized for the general purpose (GPGPU).For example, NVIDIA's GeForce7 series became a programmable processor with Cg programming language.Next GeForce8 series have CUDA as software development kit made by NVIDIA.Theoretically, calculation ability of GPU is announced as 500 GFLOPS. From the experimental result, we have achieved that 47 times faster calculation compared with our previous work which used CPU.Therefore, CGCH can be generated in 95 hours.So, total time is 110 hours to calculate and print the CGCH.

Radiation imaging with optically read out GEM-based detectors

NASA Astrophysics Data System (ADS)

Brunbauer, F. M.; Lupberger, M.; Oliveri, E.; Resnati, F.; Ropelewski, L.; Streli, C.; Thuiner, P.; van Stenis, M.

2018-02-01

Modern imaging sensors allow for high granularity optical readout of radiation detectors such as MicroPattern Gaseous Detectors (MPGDs). Taking advantage of the high signal amplification factors achievable by MPGD technologies such as Gaseous Electron Multipliers (GEMs), highly sensitive detectors can be realised and employing gas mixtures with strong scintillation yield in the visible wavelength regime, optical readout of such detectors can provide high-resolution event representations. Applications from X-ray imaging to fluoroscopy and tomography profit from the good spatial resolution of optical readout and the possibility to obtain images without the need for extensive reconstruction. Sensitivity to low-energy X-rays and energy resolution permit energy resolved imaging and material distinction in X-ray fluorescence measurements. Additionally, the low material budget of gaseous detectors and the possibility to couple scintillation light to imaging sensors via fibres or mirrors makes optically read out GEMs an ideal candidate for beam monitoring detectors in high energy physics as well as radiotherapy. We present applications and achievements of optically read out GEM-based detectors including high spatial resolution imaging and X-ray fluorescence measurements as an alternative readout approach for MPGDs. A detector concept for low intensity applications such as X-ray crystallography, which maximises detection efficiency with a thick conversion region but mitigates parallax-induced broadening is presented and beam monitoring capabilities of optical readout are explored. Augmenting high resolution 2D projections of particle tracks obtained with optical readout with timing information from fast photon detectors or transparent anodes for charge readout, 3D reconstruction of particle trajectories can be performed and permits the realisation of optically read out time projection chambers. Combining readily available high performance imaging sensors with compatible scintillating gases and the strong signal amplification factors achieved by MPGDs makes optical readout an attractive alternative to the common concept of electronic readout of radiation detectors. Outstanding signal-to-noise ratios and robustness against electronic noise allow unprecedented imaging capabilities for various applications in fields ranging from high energy physics to medical instrumentation.

Localization microscopy of DNA in situ using Vybrant(®) DyeCycle™ Violet fluorescent probe: A new approach to study nuclear nanostructure at single molecule resolution.

PubMed

Żurek-Biesiada, Dominika; Szczurek, Aleksander T; Prakash, Kirti; Mohana, Giriram K; Lee, Hyun-Keun; Roignant, Jean-Yves; Birk, Udo J; Dobrucki, Jurek W; Cremer, Christoph

2016-05-01

Higher order chromatin structure is not only required to compact and spatially arrange long chromatids within a nucleus, but have also important functional roles, including control of gene expression and DNA processing. However, studies of chromatin nanostructures cannot be performed using conventional widefield and confocal microscopy because of the limited optical resolution. Various methods of superresolution microscopy have been described to overcome this difficulty, like structured illumination and single molecule localization microscopy. We report here that the standard DNA dye Vybrant(®) DyeCycle™ Violet can be used to provide single molecule localization microscopy (SMLM) images of DNA in nuclei of fixed mammalian cells. This SMLM method enabled optical isolation and localization of large numbers of DNA-bound molecules, usually in excess of 10(6) signals in one cell nucleus. The technique yielded high-quality images of nuclear DNA density, revealing subdiffraction chromatin structures of the size in the order of 100nm; the interchromatin compartment was visualized at unprecedented optical resolution. The approach offers several advantages over previously described high resolution DNA imaging methods, including high specificity, an ability to record images using a single wavelength excitation, and a higher density of single molecule signals than reported in previous SMLM studies. The method is compatible with DNA/multicolor SMLM imaging which employs simple staining methods suited also for conventional optical microscopy. Copyright © 2016. Published by Elsevier Inc.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bose, Sownak; Li, Baojiu; He, Jian-hua

We describe and demonstrate the potential of a new and very efficient method for simulating certain classes of modified gravity theories, such as the widely studied f ( R ) gravity models. High resolution simulations for such models are currently very slow due to the highly nonlinear partial differential equation that needs to be solved exactly to predict the modified gravitational force. This nonlinearity is partly inherent, but is also exacerbated by the specific numerical algorithm used, which employs a variable redefinition to prevent numerical instabilities. The standard Newton-Gauss-Seidel iterative method used to tackle this problem has a poor convergencemore » rate. Our new method not only avoids this, but also allows the discretised equation to be written in a form that is analytically solvable. We show that this new method greatly improves the performance and efficiency of f ( R ) simulations. For example, a test simulation with 512{sup 3} particles in a box of size 512 Mpc/ h is now 5 times faster than before, while a Millennium-resolution simulation for f ( R ) gravity is estimated to be more than 20 times faster than with the old method. Our new implementation will be particularly useful for running very high resolution, large-sized simulations which, to date, are only possible for the standard model, and also makes it feasible to run large numbers of lower resolution simulations for covariance analyses. We hope that the method will bring us to a new era for precision cosmological tests of gravity.« less

Development of lidar sensor for cloud-based measurements during convective conditions

NASA Astrophysics Data System (ADS)

Vishnu, R.; Bhavani Kumar, Y.; Rao, T. Narayana; Nair, Anish Kumar M.; Jayaraman, A.

2016-05-01

Atmospheric convection is a natural phenomena associated with heat transport. Convection is strong during daylight periods and rigorous in summer months. Severe ground heating associated with strong winds experienced during these periods. Tropics are considered as the source regions for strong convection. Formation of thunder storm clouds is common during this period. Location of cloud base and its associated dynamics is important to understand the influence of convection on the atmosphere. Lidars are sensitive to Mie scattering and are the suitable instruments for locating clouds in the atmosphere than instruments utilizing the radio frequency spectrum. Thunder storm clouds are composed of hydrometers and strongly scatter the laser light. Recently, a lidar technique was developed at National Atmospheric Research Laboratory (NARL), a Department of Space (DOS) unit, located at Gadanki near Tirupati. The lidar technique employs slant path operation and provides high resolution measurements on cloud base location in real-time. The laser based remote sensing technique allows measurement of atmosphere for every second at 7.5 m range resolution. The high resolution data permits assessment of updrafts at the cloud base. The lidar also provides real-time convective boundary layer height using aerosols as the tracers of atmospheric dynamics. The developed lidar sensor is planned for up-gradation with scanning facility to understand the cloud dynamics in the spatial direction. In this presentation, we present the lidar sensor technology and utilization of its technology for high resolution cloud base measurements during convective conditions over lidar site, Gadanki.

Speeding up N-body simulations of modified gravity: chameleon screening models

NASA Astrophysics Data System (ADS)

Bose, Sownak; Li, Baojiu; Barreira, Alexandre; He, Jian-hua; Hellwing, Wojciech A.; Koyama, Kazuya; Llinares, Claudio; Zhao, Gong-Bo

2017-02-01

We describe and demonstrate the potential of a new and very efficient method for simulating certain classes of modified gravity theories, such as the widely studied f(R) gravity models. High resolution simulations for such models are currently very slow due to the highly nonlinear partial differential equation that needs to be solved exactly to predict the modified gravitational force. This nonlinearity is partly inherent, but is also exacerbated by the specific numerical algorithm used, which employs a variable redefinition to prevent numerical instabilities. The standard Newton-Gauss-Seidel iterative method used to tackle this problem has a poor convergence rate. Our new method not only avoids this, but also allows the discretised equation to be written in a form that is analytically solvable. We show that this new method greatly improves the performance and efficiency of f(R) simulations. For example, a test simulation with 5123 particles in a box of size 512 Mpc/h is now 5 times faster than before, while a Millennium-resolution simulation for f(R) gravity is estimated to be more than 20 times faster than with the old method. Our new implementation will be particularly useful for running very high resolution, large-sized simulations which, to date, are only possible for the standard model, and also makes it feasible to run large numbers of lower resolution simulations for covariance analyses. We hope that the method will bring us to a new era for precision cosmological tests of gravity.

The future of structural fieldwork - UAV assisted aerial photogrammetry

NASA Astrophysics Data System (ADS)

Vollgger, Stefan; Cruden, Alexander

2015-04-01

Unmanned aerial vehicles (UAVs), commonly referred to as drones, are opening new and low cost possibilities to acquire high-resolution aerial images and digital surface models (DSM) for applications in structural geology. UAVs can be programmed to fly autonomously along a user defined grid to systematically capture high-resolution photographs, even in difficult to access areas. The photographs are subsequently processed using software that employ SIFT (scale invariant feature transform) and SFM (structure from motion) algorithms. These photogrammetric routines allow the extraction of spatial information (3D point clouds, digital elevation models, 3D meshes, orthophotos) from 2D images. Depending on flight altitude and camera setup, sub-centimeter spatial resolutions can be achieved. By "digitally mapping" georeferenced 3D models and images, orientation data can be extracted directly and used to analyse the structural framework of the mapped object or area. We present UAV assisted aerial mapping results from a coastal platform near Cape Liptrap (Victoria, Australia), where deformed metasediments of the Palaeozoic Lachlan Fold Belt are exposed. We also show how orientation and spatial information of brittle and ductile structures extracted from the photogrammetric model can be linked to the progressive development of folds and faults in the region. Even though there are both technical and legislative limitations, which might prohibit the use of UAVs without prior commercial licensing and training, the benefits that arise from the resulting high-resolution, photorealistic models can substantially contribute to the collection of new data and insights for applications in structural geology.

High resolution aquifer characterization using crosshole GPR full-waveform tomography

NASA Astrophysics Data System (ADS)

Gueting, N.; Vienken, T.; Klotzsche, A.; Van Der Kruk, J.; Vanderborght, J.; Caers, J.; Vereecken, H.; Englert, A.

2016-12-01

Limited knowledge about the spatial distribution of aquifer properties typically constrains our ability to predict subsurface flow and transport. Here, we investigate the value of using high resolution full-waveform inversion of cross-borehole ground penetrating radar (GPR) data for aquifer characterization. By stitching together GPR tomograms from multiple adjacent crosshole planes, we are able to image, with a decimeter scale resolution, the dielectric permittivity and electrical conductivity of an alluvial aquifer along cross-sections of 50 m length and 10 m depth. A logistic regression model is employed to predict the spatial distribution of lithological facies on the basis of the GPR results. Vertical profiles of porosity and hydraulic conductivity from direct-push, flowmeter and grain size data suggest that the GPR predicted facies classification is meaningful with regard to porosity and hydraulic conductivity, even though the distributions of individual facies show some overlap and the absolute hydraulic conductivities from the different methods (direct-push, flowmeter, grain size) differ up to approximately one order of magnitude. Comparison of the GPR predicted facies architecture with tracer test data suggests that the plume splitting observed in a tracer experiment was caused by a hydraulically low-conductive sand layer with a thickness of only a few decimeters. Because this sand layer is identified by GPR full-waveform inversion but not by conventional GPR ray-based inversion we conclude that the improvement in spatial resolution due to full-waveform inversion is crucial to detect small-scale aquifer structures that are highly relevant for solute transport.

NEUTRINO-DRIVEN CONVECTION IN CORE-COLLAPSE SUPERNOVAE: HIGH-RESOLUTION SIMULATIONS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Radice, David; Ott, Christian D.; Abdikamalov, Ernazar

2016-03-20

We present results from high-resolution semiglobal simulations of neutrino-driven convection in core-collapse supernovae. We employ an idealized setup with parameterized neutrino heating/cooling and nuclear dissociation at the shock front. We study the internal dynamics of neutrino-driven convection and its role in redistributing energy and momentum through the gain region. We find that even if buoyant plumes are able to locally transfer heat up to the shock, convection is not able to create a net positive energy flux and overcome the downward transport of energy from the accretion flow. Turbulent convection does, however, provide a significant effective pressure support to the accretionmore » flow as it favors the accumulation of energy, mass, and momentum in the gain region. We derive an approximate equation that is able to explain and predict the shock evolution in terms of integrals of quantities such as the turbulent pressure in the gain region or the effects of nonradial motion of the fluid. We use this relation as a way to quantify the role of turbulence in the dynamics of the accretion shock. Finally, we investigate the effects of grid resolution, which we change by a factor of 20 between the lowest and highest resolution. Our results show that the shallow slopes of the turbulent kinetic energy spectra reported in previous studies are a numerical artifact. Kolmogorov scaling is progressively recovered as the resolution is increased.« less

Wave optics theory and 3-D deconvolution for the light field microscope

PubMed Central

Broxton, Michael; Grosenick, Logan; Yang, Samuel; Cohen, Noy; Andalman, Aaron; Deisseroth, Karl; Levoy, Marc

2013-01-01

Light field microscopy is a new technique for high-speed volumetric imaging of weakly scattering or fluorescent specimens. It employs an array of microlenses to trade off spatial resolution against angular resolution, thereby allowing a 4-D light field to be captured using a single photographic exposure without the need for scanning. The recorded light field can then be used to computationally reconstruct a full volume. In this paper, we present an optical model for light field microscopy based on wave optics, instead of previously reported ray optics models. We also present a 3-D deconvolution method for light field microscopy that is able to reconstruct volumes at higher spatial resolution, and with better optical sectioning, than previously reported. To accomplish this, we take advantage of the dense spatio-angular sampling provided by a microlens array at axial positions away from the native object plane. This dense sampling permits us to decode aliasing present in the light field to reconstruct high-frequency information. We formulate our method as an inverse problem for reconstructing the 3-D volume, which we solve using a GPU-accelerated iterative algorithm. Theoretical limits on the depth-dependent lateral resolution of the reconstructed volumes are derived. We show that these limits are in good agreement with experimental results on a standard USAF 1951 resolution target. Finally, we present 3-D reconstructions of pollen grains that demonstrate the improvements in fidelity made possible by our method. PMID:24150383

Passive microlesion detection and mapping for treatment of hypertrophic cardiomyopathy

NASA Astrophysics Data System (ADS)

Zhu, Yiying I.; Miller, Douglas L.; Dou, Chunyan; Kripfgans, Oliver D.

2017-03-01

Intermittent high intensity ultrasound pulses with circulating contrast agent microbubbles can induce scattered microlesions of potential value for myocardial reduction therapy. This paper presents an in vitro setup imitating the treatment for monitoring development. A preclinical imaging system with a single element transducer, synchronization and receive-only imaging transducer array has been implemented on a research platform. Contrast agent microbubbles pumped in a dialysis tubing setup were exposed to high intensity focused ultrasound at 1.0/3.5 MHz center frequencies. Polystyrene spheres were employed as linear scatterers compared to contrast agents for system transfer function equalization. A cavitation mapping technique was employed to spatially locate and depict microbubble activity during treatment. For high acoustic pressure amplitudes a 5 dB difference between contrast agent and solid spheres was observed and spatially mapped. The in-plane resolution was 4.5 mm for axial and 1.5 mm laterally. In the future, this cavitation detection scheme will be applied to monitor in vivo microlesioning in real-time.

KiwiSpec - an advanced spectrograph for high resolution spectroscopy: optical design and variations

NASA Astrophysics Data System (ADS)

Barnes, Stuart I.; Gibson, Steve; Nield, Kathryn; Cochrane, Dave

2012-09-01

The KiwiSpec R4-100 is an advanced high resolution spectrograph developed by KiwiStar Optics, Industrial Research Ltd, New Zealand. The instrument is based around an R4 echelle grating and a 100mm collimated beam diameter. The optical design employs a highly asymmetric white pupil design, whereby the transfer collimator has a focal length only 1/3 that of the primary collimator. This allows the cross-dispersers (VPH gratings) and camera optics to be small and low cost while also ensuring a very compact instrument. The KiwiSpec instrument will be bre-fed and is designed to be contained in both thermal and/or vacuum enclosures. The instrument concept is highly exible in order to ensure that the same basic design can be used for a wide variety of science cases. Options include the possibility of splitting the wavelength coverage into 2 to 4 separate channels allowing each channel to be highly optimized for maximum eciency. CCDs ranging from smaller than 2K2K to larger than 4K4K can be accommodated. This allows good (3-4 pixel) sampling of resolving powers ranging from below 50,000 to greater than 100,000. Among the specic design options presented here will be a two-channel concept optimized for precision radial velocities, and a four-channel concept developed for the Gemini High- Resolution Optical Spectrograph (GHOST). The design and performance of a single-channel prototype will be presented elsewhere in these proceedings.

Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search.

PubMed

Li, Tingting; Cao, Jingjing; Li, Zhen; Wang, Xian; He, Pingli

2016-02-01

Broad screening and identification of β-agonists in feed, serum, urine, muscle and liver samples was achieved in a quick and highly sensitive manner using ultra high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) combined with a spectra library search. Solid-phase extraction technology was employed for sample purification and enrichment. After extraction and purification, the samples were analyzed using a Q-Orbitrap high-resolution mass spectrometer under full-scan and data-dependent MS/MS mode. The acquired mass spectra were compared with an in-house library (compound library and MS/MS mass spectral library) built with TraceFinder Software which contained the M/Z of the precursor ion, chemical formula, retention time, character fragment ions and the entire MS/MS spectra of 32 β-agonist standards. Screening was achieved by comparing 5 key mass spectral results and positive matches were marked. Using the developed method, the identification results from 10 spiked samples and 238 actual samples indicated that only 2% of acquired mass spectra produced false identities. The method validation results showed that the limit of detection ranged from 0.021-3.854 μg kg(-1)and 0.015-1.198 ng mL(-1) for solid and liquid samples, respectively. Copyright © 2015 Elsevier Ltd. All rights reserved.

Fingerprints of flower absolutes using supercritical fluid chromatography hyphenated with high resolution mass spectrometry.

PubMed

Santerre, Cyrille; Vallet, Nadine; Touboul, David

2018-06-02

Supercritical fluid chromatography hyphenated with high resolution mass spectrometry (SFC-HRMS) was developed for fingerprint analysis of different flower absolutes commonly used in cosmetics field, especially in perfumes. Supercritical fluid chromatography-atmospheric pressure photoionization-high resolution mass spectrometry (SFC-APPI-HRMS) technique was employed to identify the components of the fingerprint. The samples were separated with a porous graphitic carbon (PGC) Hypercarb™ column (100 mm × 2.1 mm, 3 μm) by gradient elution using supercritical CO 2 and ethanol (0.0-20.0 min (2-30% B), 20.0-25.0 min (30% B), 25.0-26.0 min (30-2% B) and 26.0-30.0 min (2% B)) as mobile phase at a flow rate of 1.5 mL/min. In order to compare the SFC fingerprints between five different flower absolutes: Jasminum grandiflorum absolutes, Jasminum sambac absolutes, Narcissus jonquilla absolutes, Narcissus poeticus absolutes, Lavandula angustifolia absolutes from different suppliers and batches, the chemometric procedure including principal component analysis (PCA) was applied to classify the samples according to their genus and their species. Consistent results were obtained to show that samples could be successfully discriminated. Copyright © 2018 Elsevier B.V. All rights reserved.

Characterization of conductive nanobiomaterials derived from viral assemblies by low-voltage STEM imaging and Raman scattering

NASA Astrophysics Data System (ADS)

Plascencia-Villa, Germán; Carreño-Fuentes, Liliana; Bahena, Daniel; José-Yacamán, Miguel; Palomares, Laura A.; Ramírez, Octavio T.

2014-09-01

New technologies require the development of novel nanomaterials that need to be fully characterized to achieve their potential. High-resolution low-voltage scanning transmission electron microscopy (STEM) has proven to be a very powerful technique in nanotechnology, but its use for the characterization of nanobiomaterials has been limited. Rotavirus VP6 self-assembles into nanotubular assemblies that possess an intrinsic affinity for Au ions. This property was exploited to produce hybrid nanobiomaterials by the in situ functionalization of recombinant VP6 nanotubes with gold nanoparticles. In this work, Raman spectroscopy and advanced analytical electron microscopy imaging with spherical aberration-corrected (Cs) STEM and nanodiffraction at low-voltage doses were employed to characterize nanobiomaterials. STEM imaging revealed the precise structure and arrangement of the protein templates, as well as the nanostructure and atomic arrangement of gold nanoparticles with high spatial sub-Angstrom resolution and avoided radiation damage. The imaging was coupled with backscattered electron imaging, ultra-high resolution scanning electron microscopy and x-ray spectroscopy. The hybrid nanobiomaterials that were obtained showed unique properties as bioelectronic conductive devices and showed enhanced Raman scattering by their precise arrangement into superlattices, displaying the utility of viral assemblies as functional integrative self-assembled nanomaterials for novel applications.

Method of LSD profile asymmetry for estimating the center of mass velocities of pulsating stars

NASA Astrophysics Data System (ADS)

Britavskiy, Nikolay; Pancino, Elena; Romano, Donatella; Tsymbal, Vadim

2015-08-01

We present radial velocity analysis for 20 solar neighborhood RR Lyrae and 3 Population II Cepheids. High-resolution spectra were observed with either TNG/SARG or VLT/UVES over varying phases. To estimate the center of mass (barycentric) velocities of the program stars, we utilized two independent methods. First, the 'classic' method was employed, which is based on RR Lyrae radial velocity curve templates. Second, we provide the new method that used absorption line profile asymmetry to determine both the pulsation and the barycentric velocities even with a low number of high-resolution spectra and in cases where the phase of the observations is uncertain. This new method is based on a Least Squares Deconvolution (LSD) of the line profiles in order to analyze line asymmetry that occurs in the spectra of pulsating stars. By applying this method to our sample stars we attain accurate measurements (± 1 km/s) of the pulsation component of the radial velocity. This results in determination of the barycentric velocity to within 5 km/s even with a low number of high-resolution spectra. A detailed investigation of LSD profile asymmetry shows the variable nature of the project factor at different pulsation phases, which should be taken into account in the detailed spectroscopic analysis of pulsating stars.

Method of LSD profile asymmetry for estimating the center of mass velocities of pulsating stars

NASA Astrophysics Data System (ADS)

Britavskiy, N.; Pancino, E.; Tsymbal, V.; Romano, D.; Cacciari, C.; Clementini, C.

2016-05-01

We present radial velocity analysis for 20 solar neighborhood RR Lyrae and 3 Population II Cepheids. High-resolution spectra were observed with either TNG/SARG or VLT/UVES over varying phases. To estimate the center of mass (barycentric) velocities of the program stars, we utilized two independent methods. First, the 'classic' method was employed, which is based on RR Lyrae radial velocity curve templates. Second, we provide the new method that used absorption line profile asymmetry to determine both the pulsation and the barycentric velocities even with a low number of high-resolution spectra and in cases where the phase of the observations is uncertain. This new method is based on a least squares deconvolution (LSD) of the line profiles in order to an- alyze line asymmetry that occurs in the spectra of pulsating stars. By applying this method to our sample stars we attain accurate measurements (+- 2 kms^-1) of the pulsation component of the radial velocity. This results in determination of the barycentric velocity to within 5 kms^-1 even with a low number of high- resolution spectra. A detailed investigation of LSD profile asymmetry shows the variable nature of the project factor at different pulsation phases, which should be taken into account in the detailed spectroscopic analysis of pulsating stars.

Polyacrylamide medium for the electrophoretic separation of biomolecules

DOEpatents

Madabhushi, Ramakrishna S.; Gammon, Stuart A.

2003-11-11

A polyacryalmide medium for the electrophoretic separation of biomolecules. The polyacryalmide medium comprises high molecular weight polyacrylamides (PAAm) having a viscosity average molecular weight (M.sub.v) of about 675-725 kDa were synthesized by conventional red-ox polymerization technique. Using this separation medium, capillary electrophoresis of BigDye DNA sequencing standard was performed. A single base resolution of .about.725 bases was achieved in .about.60 minute in a non-covalently coated capillary of 50 .mu.m i.d., 40 cm effective length, and a filed of 160 V/cm at 40.degree. C. The resolution achieved with this formulation to separate DNA under identical conditions is much superior (725 bases vs. 625 bases) and faster (60 min. vs. 75 min.) to the commercially available PAAm, such as supplied by Amersham. The formulation method employed here to synthesize PAAm is straight-forward, simple and does not require cumbersome methods such as emulsion polymerizaiton in order to achieve very high molecular weights. Also, the formulation here does not require separation of PAAm from the reaction mixture prior to reconstituting the polymer to a final concentration. Furthermore, the formulation here is prepared from a single average mol. wt. PAAm as opposed to the mixture of two different average mo. wt. PAAm previously required to achieve high resolution.

Moist Thermodynamics of Tropical Cyclone Formation and Intensification in High-Resolution Climate Models

NASA Astrophysics Data System (ADS)

Wing, A. A.; Camargo, S. J.; Sobel, A. H.; Kim, D.; Moon, Y.; Bosilovich, M. G.; Murakami, H.; Reed, K. A.; Vecchi, G. A.; Wehner, M. F.; Zarzycki, C. M.; Zhao, M.

2017-12-01

In recent years, climate models have improved such that high-resolution simulations are able to reproduce the climatology of tropical cyclone activity with some fidelity and show some skill in seasonal forecasting. However, biases remain in many models, motivating a better understanding of what factors control the representation of tropical cyclone activity in climate models. We explore tropical cyclogenesis and intensification processes in six high-resolution climate models from NOAA/GFDL, NCAR, and NASA, including both coupled and uncoupled configurations. Our analysis framework focuses on how convection, moisture, clouds and related processes are coupled and employs budgets of column moist static energy and the spatial variance of column moist static energy. The latter allows us to quantify the different feedback processes responsible for the amplification of moist static energy anomalies associated with the organization of convection and cyclogenesis, including surface flux feedbacks and cloud-radiative feedbacks. We track the formation and evolution of tropical cyclones in the climate model simulations and apply our analysis along the individual tracks and composited over many tropical cyclones. We use two methods of compositing: a composite over all TC track points in a given intensity range, and a composite relative to the time of lifetime maximum intensity for each storm (at the same stage in the TC life cycle).

Design and image-quality performance of high resolution CMOS-based X-ray imaging detectors for digital mammography

NASA Astrophysics Data System (ADS)

Cha, B. K.; Kim, J. Y.; Kim, Y. J.; Yun, S.; Cho, G.; Kim, H. K.; Seo, C.-W.; Jeon, S.; Huh, Y.

2012-04-01

In digital X-ray imaging systems, X-ray imaging detectors based on scintillating screens with electronic devices such as charge-coupled devices (CCDs), thin-film transistors (TFT), complementary metal oxide semiconductor (CMOS) flat panel imagers have been introduced for general radiography, dental, mammography and non-destructive testing (NDT) applications. Recently, a large-area CMOS active-pixel sensor (APS) in combination with scintillation films has been widely used in a variety of digital X-ray imaging applications. We employed a scintillator-based CMOS APS image sensor for high-resolution mammography. In this work, both powder-type Gd2O2S:Tb and a columnar structured CsI:Tl scintillation screens with various thicknesses were fabricated and used as materials to convert X-ray into visible light. These scintillating screens were directly coupled to a CMOS flat panel imager with a 25 × 50 mm2 active area and a 48 μm pixel pitch for high spatial resolution acquisition. We used a W/Al mammographic X-ray source with a 30 kVp energy condition. The imaging characterization of the X-ray detector was measured and analyzed in terms of linearity in incident X-ray dose, modulation transfer function (MTF), noise-power spectrum (NPS) and detective quantum efficiency (DQE).

Ptychographic imaging with partially coherent plasma EUV sources

NASA Astrophysics Data System (ADS)

Bußmann, Jan; Odstrčil, Michal; Teramoto, Yusuke; Juschkin, Larissa

2017-12-01

We report on high-resolution lens-less imaging experiments based on ptychographic scanning coherent diffractive imaging (CDI) method employing compact plasma sources developed for extreme ultraviolet (EUV) lithography applications. Two kinds of discharge sources were used in our experiments: a hollow-cathode-triggered pinch plasma source operated with oxygen and for the first time a laser-assisted discharge EUV source with a liquid tin target. Ptychographic reconstructions of different samples were achieved by applying constraint relaxation to the algorithm. Our ptychography algorithms can handle low spatial coherence and broadband illumination as well as compensate for the residual background due to plasma radiation in the visible spectral range. Image resolution down to 100 nm is demonstrated even for sparse objects, and it is limited presently by the sample structure contrast and the available coherent photon flux. We could extract material properties by the reconstruction of the complex exit-wave field, gaining additional information compared to electron microscopy or CDI with longer-wavelength high harmonic laser sources. Our results show that compact plasma-based EUV light sources of only partial spatial and temporal coherence can be effectively used for lens-less imaging applications. The reported methods may be applied in combination with reflectometry and scatterometry for high-resolution EUV metrology.

High-resolution slab gel isoelectric focusing: methods for quantitative electrophoretic transfer and immunodetection of proteins as applied to the study of the multiple isoelectric forms of ornithine decarboxylase.

PubMed

Reddy, S G; Cochran, B J; Worth, L L; Knutson, V P; Haddox, M K

1994-04-01

A high-resolution isoelectric focusing vertical slab gel method which can resolve proteins which differ by a single charge was developed and this method was applied to the study of the multiple isoelectric forms of ornithine decarboxylase. Separation of proteins at this high level of resolution was achieved by increasing the ampholyte concentration in the gels to 6%. Various lots of ampholytes, from the same or different commercial sources, differed significantly in their protein binding capacity. Ampholytes bound to proteins interfered both with the electrophoretic transfer of proteins from the gel to immunoblotting membranes and with the ability of antibodies to interact with proteins on the immunoblotting membranes. Increasing the amount of protein loaded into a gel lane also decreased the efficiency of the electrophoretic transfer and immunodetection. To overcome these problems, both gel washing and gel electrophoretic transfer protocols for disrupting the ampholyte-protein binding and enabling a quantitative electrophoretic transfer of proteins were developed. Two gel washing procedures, with either thiocyanate or borate buffers, and a two-step electrophoretic transfer method are described. The choice of which method to use to optimally disrupt the ampholyte-protein binding was found to vary with each lot of ampholytes employed.

A double-cusp type electrostatic analyzer for high-cadence ring current ion measurements

NASA Astrophysics Data System (ADS)

Ogasawara, K.; Allegrini, F.; Burch, J. L.; Desai, M. I.; Ebert, R. W.; Goldstein, J.; John, J. M.; Livi, S. A.; McComas, D. J.

2015-12-01

Detailed observations of a variety of ion species at a sufficiently high temporal resolution are essential to understanding the loss and acceleration processes of ring current ions. For example, CRESS/MICS observations indicated that the energy density of suprathermal O+ exceeds that of H+ in large magnetic storms (Daglis et al., 1997), while the H+ energy density dominates under quiet conditions. However, the primary ion loss processes during the storm recovery phase are still incompletely understood. The mechanisms to accelerate upflowing ions, regularly observed with energies of a few keV at ~1000 km altitude, up to the 100s-keV range in the geospace are also not fully understood. Our novel electrostatic analyzer (ESA) employs a toroidal double-shell structure to cover the entire ring current ion range of ~3-250 keV/Q with high temporal resolution (<1 minute with a necessary counting statistics for the quiet time), while saving significant resources in mass and size. In this presentation, we discuss the operation principle and the proof of concept study of the ESA in terms of numerical calculations and ion beam calibration activities. This presentation comprehensively covers the expected sensor performance important for the in-flight capabilities, such as sensor parameters (G-factor, K-factor, and energy resolution), cross-shell contaminations, and UV background counts.

Development and Characterization of a Dither-Based Super-Resolution Reconstruction Method for Fiber Imaging Arrays

NASA Astrophysics Data System (ADS)

Languirand, Eric Robert

Chemical imaging is an important tool for providing insight into function, role, and spatial distribution of analytes. This thesis describes the use of imaging fiber bundles (IFB) for super-resolution reconstruction using surface enhanced Raman scattering (SERS) showing improvement in resolution with arrayed bundles for the first time. Additionally this thesis describes characteristics of the IFB with regards to cross-talk as a function of aperture size. The first part of this thesis characterizes the IFB for both tapered and untapered bundles in terms of cross-talk. Cross-talk is defined as the amount of light leaking from a central fiber element in the imaging fiber bundle to surrounding fiber elements. To make this measurement ubiquitous for all imaging bundles, quantum dots were employed. Untapered and tapered IFB possess cross-talk of 2% or less, with fiber elements down to 32nm. The second part of this thesis employs a super resolution reconstruction algorithm using projection onto convex sets for resolution improvement. When using IFB arrays, the point spread function (PSF) of the array can be known accurately if the fiber elements over fill the pixel detector array. Therefore, the use of the known PSF compared to a general blurring kernel was evaluated. Relative increases in resolution of 12% and 2% at the 95% confidence level are found, when compared to a reference image, for the general blurring kernel and PSF, respectively. The third part of this thesis shows for the first time the use of SERS with a dithered IFB array coupled with super-resolution reconstruction. The resolution improvement across a step-edge is shown to be approximately 20% when compared to a reference image. This provides an additional means of increasing the resolution of fiber bundles beyond that of just tapering. Furthermore, this provides a new avenue for nanoscale imaging using these bundles. Lastly, synthetic data with varying degrees of signal-to-noise (S/N) were employed to explore the relationship S/N has with the reconstruction process. It is generally shown that increasing the number images used in the reconstruction process and increasing the S/N will improve the reconstruction providing larger increases in resolution.

Matrix-effect free multi-residue analysis of veterinary drugs in food samples of animal origin by nanoflow liquid chromatography high resolution mass spectrometry.

PubMed

Alcántara-Durán, Jaime; Moreno-González, David; Gilbert-López, Bienvenida; Molina-Díaz, Antonio; García-Reyes, Juan F

2018-04-15

In this work, a sensitive method based on nanoflow liquid chromatography high-resolution mass spectrometry has been developed for the multiresidue determination of veterinary drugs residues in honey, veal muscle, egg and milk. Salting-out supported liquid extraction was employed as sample treatment for milk, veal muscle and egg, while a modified QuEChERS procedure was used in honey. The enhancement of sensitivity provided by the nanoflow LC system also allowed the implementation of high dilution factors as high as 100:1. For all matrices tested, matrix effects were negligible starting from a dilution factor of 100, enabling, thus, the use of external standard calibration instead of matrix-matched calibration of each sample, and the subsequent increase of laboratory throughput. At spiked levels as low as 0.1 or 1 µg kg -1 before the 1:100 dilution, the obtained signals were still significantly higher than the instrumental limit of quantitation (S/N 10). Copyright © 2017 Elsevier Ltd. All rights reserved.

High resolution identity testing of inactivated poliovirus vaccines

PubMed Central

Mee, Edward T.; Minor, Philip D.; Martin, Javier

2015-01-01

Background Definitive identification of poliovirus strains in vaccines is essential for quality control, particularly where multiple wild-type and Sabin strains are produced in the same facility. Sequence-based identification provides the ultimate in identity testing and would offer several advantages over serological methods. Methods We employed random RT-PCR and high throughput sequencing to recover full-length genome sequences from monovalent and trivalent poliovirus vaccine products at various stages of the manufacturing process. Results All expected strains were detected in previously characterised products and the method permitted identification of strains comprising as little as 0.1% of sequence reads. Highly similar Mahoney and Sabin 1 strains were readily discriminated on the basis of specific variant positions. Analysis of a product known to contain incorrect strains demonstrated that the method correctly identified the contaminants. Conclusion Random RT-PCR and shotgun sequencing provided high resolution identification of vaccine components. In addition to the recovery of full-length genome sequences, the method could also be easily adapted to the characterisation of minor variant frequencies and distinction of closely related products on the basis of distinguishing consensus and low frequency polymorphisms. PMID:26049003

Real-time monitoring of laser powder bed fusion process using high-speed X-ray imaging and diffraction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, Cang; Fezzaa, Kamel; Cunningham, Ross W.

Here, we employ the high-speed synchrotron hard X-ray imaging and diffraction techniques to monitor the laser powder bed fusion (LPBF) process of Ti-6Al-4V in situ and in real time. We demonstrate that many scientifically and technologically significant phenomena in LPBF, including melt pool dynamics, powder ejection, rapid solidification, and phase transformation, can be probed with unprecedented spatial and temporal resolutions. In particular, the keyhole pore formation is experimentally revealed with high spatial and temporal resolutions. The solidification rate is quantitatively measured, and the slowly decrease in solidification rate during the relatively steady state could be a manifestation of the recalescencemore » phenomenon. The high-speed diffraction enables a reasonable estimation of the cooling rate and phase transformation rate, and the diffusionless transformation from β to α’ phase is evident. The data present here will facilitate the understanding of dynamics and kinetics in metal LPBF process, and the experiment platform established will undoubtedly become a new paradigm for future research and development of metal additive manufacturing.« less

Annual variation of gravity-wave activity at middle and high latitudes in a high-resolution GCM

NASA Astrophysics Data System (ADS)

Becker, E.

2017-12-01

A high-resolution version of the Kuehlungsborn Mechanistic general Circulation Model (KMCM) with resolved gravity waves (GWs) is employed to analyze the annual variation of GW activity in both hemispheres at middle and high latitudes. The geographical distributions of GW hotspots in the winter stratosphere are consistent with existing satellite data. Vertical profiles up to the lower thermosphere agree with ground-based measurements for both season. The model confirms the semi-annual variation of GW energy in the upper mesosphere that was found previously in radar-measurements in the northern hemisphere Furthermore, the GW potential energy per unit mass during winter shows two maxima, one around 50-70 km and one around 80-100 km. We interpret the upper maximum as a result of secondary GWs that are generated in the stratopause region due to the intermittent body forces of primary GWs. In a recent study we showed that these secondary GWs produce a significant eastward drag in the mesopause region during austral winter. This mechanism is found to be important in the northern winter as well.

Graphene-ZIF8 composite material as stationary phase for high-resolution gas chromatographic separations of aliphatic and aromatic isomers.

PubMed

Yang, Xiaohong; Li, Changxia; Qi, Meiling; Qu, Liangti

2016-08-19

This work presents the separation performance of graphene-ZIF8 (G-Z) composite material as stationary phase for capillary gas chromatography (GC). The G-Z stationary phase achieved high column efficiency of 5000 plates/m determined by n-dodecane (k=1.22) at 120°C and showed weakly polar nature. Importantly, it exhibited high selectivity and resolving capability for branched alkane isomers and aromatic positional isomers, showing clear advantages over the reported neat graphene and ZIF8. In addition, it attained high resolution for geometric cis-/trans-isomers. The G-Z column exhibited good column thermal stability up to 300°C and column repeatability with RSD values of retention times in the range of 0.01-0.19% for intra-day, 0.05-0.88% for inter-day and 0.66-5.6% for between-column, respectively, Moreover, the G-Z column was employed for the determination of minor impurity isomers in real reagent samples, which demonstrates its promising potential in GC applications. Copyright © 2016 Elsevier B.V. All rights reserved.

Real-time monitoring of laser powder bed fusion process using high-speed X-ray imaging and diffraction

DOE PAGES

Zhao, Cang; Fezzaa, Kamel; Cunningham, Ross W.; ...

2017-06-15

Here, we employ the high-speed synchrotron hard X-ray imaging and diffraction techniques to monitor the laser powder bed fusion (LPBF) process of Ti-6Al-4V in situ and in real time. We demonstrate that many scientifically and technologically significant phenomena in LPBF, including melt pool dynamics, powder ejection, rapid solidification, and phase transformation, can be probed with unprecedented spatial and temporal resolutions. In particular, the keyhole pore formation is experimentally revealed with high spatial and temporal resolutions. The solidification rate is quantitatively measured, and the slowly decrease in solidification rate during the relatively steady state could be a manifestation of the recalescencemore » phenomenon. The high-speed diffraction enables a reasonable estimation of the cooling rate and phase transformation rate, and the diffusionless transformation from β to α’ phase is evident. The data present here will facilitate the understanding of dynamics and kinetics in metal LPBF process, and the experiment platform established will undoubtedly become a new paradigm for future research and development of metal additive manufacturing.« less

Acoustic focusing by symmetrical self-bending beams with phase modulations

NASA Astrophysics Data System (ADS)

Gao, He; Gu, Zhong-ming; Liang, Bin; Zou, Xin-ye; Yang, Jing; Yang, Jun; Cheng, Jian-chun

2016-02-01

We propose a scheme for generating high-efficient acoustic focusing capable of circumventing obstacles in the propagating medium. This distinct feature that is highly desirable for practical applications is realized by employing two symmetrical Airy beams, and a different type of acoustic lens is designed by using a zero-index medium to provide the required phase profile with extremely high resolution. Furthermore, the scheme has the flexibility of generating tunable focal length. We anticipate our design to open possibilities for the design of acoustic lens and have potential applications in various important scenarios such as biomedical imaging/therapy and non-destructive evaluation.

Polarization- and wavelength-resolved near-field imaging of complex plasmonic modes in Archimedean nanospirals

DOE PAGES

Hachtel, Jordan A.; Davidson, II, Roderick B.; Kovalik, Elena R.; ...

2018-02-15

Asymmetric nanophotonic structures enable a wide range of opportunities in optical nanotechnology because they support efficient optical nonlinearities mediated by multiple plasmon resonances over a broad spectral range. The Archimedean nanospiral is a canonical example of a chiral plasmonic structure because it supports even-order nonlinearities that are not generally accessible in locally symmetric geometries. However, the complex spiral response makes nanoscale experimental characterization of the plasmonic near-field structure highly desirable. As a result, we employ high-efficiency, high-spatial-resolution cathodoluminescence imaging in a scanning transmission electron microscope to describe the spatial, spectral, and polarization response of plasmon modes in the nanospiral geometry.

Polarization- and wavelength-resolved near-field imaging of complex plasmonic modes in Archimedean nanospirals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hachtel, Jordan A.; Davidson, II, Roderick B.; Kovalik, Elena R.

Asymmetric nanophotonic structures enable a wide range of opportunities in optical nanotechnology because they support efficient optical nonlinearities mediated by multiple plasmon resonances over a broad spectral range. The Archimedean nanospiral is a canonical example of a chiral plasmonic structure because it supports even-order nonlinearities that are not generally accessible in locally symmetric geometries. However, the complex spiral response makes nanoscale experimental characterization of the plasmonic near-field structure highly desirable. As a result, we employ high-efficiency, high-spatial-resolution cathodoluminescence imaging in a scanning transmission electron microscope to describe the spatial, spectral, and polarization response of plasmon modes in the nanospiral geometry.

Verification of High Resolution Soil Moisture and Latent Heat in Germany

NASA Astrophysics Data System (ADS)

Samaniego, L. E.; Warrach-Sagi, K.; Zink, M.; Wulfmeyer, V.

2012-12-01

Improving our understanding of soil-land-surface-atmosphere feedbacks is fundamental to make reliable predictions of water and energy fluxes on land systems influenced by anthropogenic activities. Estimating, for instance, which would be the likely consequences of changing climatic regimes on water availability and crop yield, requires of high resolution soil moisture. Modeling it at large-scales, however, is difficult and uncertain because of the interplay between state variables and fluxes and the significant parameter uncertainty of the predicting models. At larger scales, the sub-grid variability of the variables involved and the nonlinearity of the processes complicate the modeling exercise even further because parametrization schemes might be scale dependent. Two contrasting modeling paradigms (WRF/Noah-MP and mHM) were employed to quantify the effects of model and data complexity on soil moisture and latent heat over Germany. WRF/Noah-MP was forced ERA-interim on the boundaries of the rotated CORDEX-Grid (www.meteo.unican.es/wiki/cordexwrf) with a spatial resolution of 0.11o covering Europe during the period from 1989 to 2009. Land cover and soil texture were represented in WRF/Noah-MP with 1×1~km MODIS images and a single horizon, coarse resolution European-wide soil map with 16 soil texture classes, respectively. To ease comparison, the process-based hydrological model mHM was forced with daily precipitation and temperature fields generated by WRF during the same period. The spatial resolution of mHM was fixed at 4×4~km. The multiscale parameter regionalization technique (MPR, Samaniego et al. 2010) was embedded in mHM to be able to estimate effective model parameters using hyper-resolution input data (100×100~km) obtained from Corine land cover and detailed soil texture fields for various horizons comprising 72 soil texture classes for Germany, among other physiographical variables. mHM global parameters, in contrast with those of Noah-MP, were obtained by closing the water balance over major river basins in Germany. Simulated soil moisture and latent heat flux were also evaluated at several eddy covariance sites in Germany. Comparison of monthly soil moisture and latent heat fields obtained with both models over Germany exhibited significant differences, which are mainly attributed to the subgrid variability of key model parameters such as porosity and aerodynamic resistance. Comparison of soil moisture fields obtained with WRF/Noah-MP and mHM forced with grided metereological observations (German Meteorological Service) showed that the differences between both models are mainly due to a combination of precipitation bias and different soil texture resolution. However, EOF analyses indicate that CORDEX results start recovering structures due to soil and vegetation properties. This experiment clearly highlighted the importance of hyper resolution input data to address these challenge. High resolution mHM simulations also indicate that the parametric uncertainty of land surface models is significant, and should not be neglected if a model is to be employed for application at regional scales, e.g. for drought monitoring.

Close-range photogrammetric reconstruction of moraine dam failures

NASA Astrophysics Data System (ADS)

Westoby, M. J.; Brasington, J.; Glasser, N. F.; Hambrey, M. J.; Reynolds, J. M.

2012-04-01

Glacial Lake Outburst Floods (GLOFs) from moraine-dammed lakes represent a high magnitude, low frequency catastrophic glacio-fluvial phenomena, with the potential to cause significant damage to property and infrastructure in high-mountain regions. Detailed accounts of GLOF dynamics, in particular the initiation and propagation of dam breaching are extremely rare, owing to their occurrence in often remote, inaccessible areas, as well as the impracticalities associated with attempting to directly instrument such high magnitude, turbulent flows. In addition to the dearth of detailed, first-hand observations of dam failures, reconstruction of breaches and failure mechanisms derived from morphological evidence is hampered by the lack of high-quality, high-resolution DTMs of remote alpine areas. Previous studies have therefore resorted to the use of coarse resolution data products (SRTM, ASTER GDEM) to quantify characteristics of failure events, e.g. pre-flood lake volume, dam height/width, which may give rise to considerable uncertainty in related numerical simulations and assessments of downstream flood hazards. In this paper we employ a novel low-cost, close-range photogrammetric technique, termed 'Structure-from-Motion' (SfM) to provide detailed in-situ reconstructions of dam and valley topography for two moraine dam complexes which have produced historical GLOFs in the Khumbu Himal, Nepal. Requiring little more than a consumer-grade digital camera and suitable ground control for implementation, the resolution of the final data products are comparable to that obtained using ground-based or airborne LiDAR. These data facilitate the extraction of precise estimates of dam (and breach) geometry, volumes of water and sediment removed during the outburst events, and the downstream channel topography. We conclude by directly comparing such key metrics derived from low-resolution topographic datasets, with those acquired in situ using the SfM technique, and discuss the implications for the reconstruction of flood dynamics.

High-resolution myocardial T1 mapping using single-shot inversion recovery fast low-angle shot MRI with radial undersampling and iterative reconstruction

PubMed Central

Joseph, Arun A; Kalentev, Oleksandr; Merboldt, Klaus-Dietmar; Voit, Dirk; Roeloffs, Volkert B; van Zalk, Maaike; Frahm, Jens

2016-01-01

Objective: To develop a novel method for rapid myocardial T1 mapping at high spatial resolution. Methods: The proposed strategy represents a single-shot inversion recovery experiment triggered to early diastole during a brief breath-hold. The measurement combines an adiabatic inversion pulse with a real-time readout by highly undersampled radial FLASH, iterative image reconstruction and T1 fitting with automatic deletion of systolic frames. The method was implemented on a 3-T MRI system using a graphics processing unit-equipped bypass computer for online application. Validations employed a T1 reference phantom including analyses at simulated heart rates from 40 to 100 beats per minute. In vivo applications involved myocardial T1 mapping in short-axis views of healthy young volunteers. Results: At 1-mm in-plane resolution and 6-mm section thickness, the inversion recovery measurement could be shortened to 3 s without compromising T1 quantitation. Phantom studies demonstrated T1 accuracy and high precision for values ranging from 300 to 1500 ms and up to a heart rate of 100 beats per minute. Similar results were obtained in vivo yielding septal T1 values of 1246 ± 24 ms (base), 1256 ± 33 ms (mid-ventricular) and 1288 ± 30 ms (apex), respectively (mean ± standard deviation, n = 6). Conclusion: Diastolic myocardial T1 mapping with use of single-shot inversion recovery FLASH offers high spatial resolution, T1 accuracy and precision, and practical robustness and speed. Advances in knowledge: The proposed method will be beneficial for clinical applications relying on native and post-contrast T1 quantitation. PMID:27759423

High-resolution simulation of link-level vehicle emissions and concentrations for air pollutants in a traffic-populated eastern Asian city

NASA Astrophysics Data System (ADS)

Zhang, Shaojun; Wu, Ye; Huang, Ruikun; Wang, Jiandong; Yan, Han; Zheng, Yali; Hao, Jiming

2016-08-01

Vehicle emissions containing air pollutants created substantial environmental impacts on air quality for many traffic-populated cities in eastern Asia. A high-resolution emission inventory is a useful tool compared with traditional tools (e.g. registration data-based approach) to accurately evaluate real-world traffic dynamics and their environmental burden. In this study, Macau, one of the most populated cities in the world, is selected to demonstrate a high-resolution simulation of vehicular emissions and their contribution to air pollutant concentrations by coupling multimodels. First, traffic volumes by vehicle category on 47 typical roads were investigated during weekdays in 2010 and further applied in a networking demand simulation with the TransCAD model to establish hourly profiles of link-level vehicle counts. Local vehicle driving speed and vehicle age distribution data were also collected in Macau. Second, based on a localized vehicle emission model (e.g. the emission factor model for the Beijing vehicle fleet - Macau, EMBEV-Macau), this study established a link-based vehicle emission inventory in Macau with high resolution meshed in a temporal and spatial framework. Furthermore, we employed the AERMOD (AMS/EPA Regulatory Model) model to map concentrations of CO and primary PM2.5 contributed by local vehicle emissions during weekdays in November 2010. This study has discerned the strong impact of traffic flow dynamics on the temporal and spatial patterns of vehicle emissions, such as a geographic discrepancy of spatial allocation up to 26 % between THC and PM2.5 emissions owing to spatially heterogeneous vehicle-use intensity between motorcycles and diesel fleets. We also identified that the estimated CO2 emissions from gasoline vehicles agreed well with the statistical fuel consumption in Macau. Therefore, this paper provides a case study and a solid framework for developing high-resolution environment assessment tools for other vehicle-populated cities in eastern Asia.

A comparison of the IGBP DISCover and University of Maryland 1 km global land cover products

USGS Publications Warehouse

Hansen, M.C.; Reed, B.

2000-01-01

Two global 1 km land cover data sets derived from 1992-1993 Advanced Very High Resolution Radiometer (AVHRR) data are currently available, the International Geosphere-Biosphere Programme Data and Information System (IGBP-DIS) DISCover and the University of Maryland (UMd) 1 km land cover maps. This paper makes a preliminary comparison of the methodologies and results of the two products. The DISCover methodology employed an unsupervised clustering classification scheme on a per-continent basis using 12 monthly maximum NDVI composites as inputs. The UMd approach employed a supervised classification tree method in which temporal metrics derived from all AVHRR bands and the NDVI were used to predict class membership across the entire globe. The DISCover map uses the IGBP classification scheme, while the UMd map employs a modified IGBP scheme minus the classes of permanent wetlands, cropland/natural vegetation mosaic and ice and snow. Global area totals of aggregated vegetation types are very similar and have a per-pixel agreement of 74%. For tall versus short/no vegetation, the per-pixel agreement is 84%. For broad vegetation types, core areas map similarly, while transition zones around core areas differ significantly. This results in high regional variability between the maps. Individual class agreement between the two 1 km maps is 49%. Comparison of the maps at a nominal 0.5 resolution with two global ground-based maps shows an improvement of thematic concurrency of 46% when viewing average class agreement. The absence of the cropland mosaic class creates a difficulty in comparing the maps, due to its significant extent in the DISCover map. The DISCover map, in general, has more forest, while the UMd map has considerably more area in the intermediate tree cover classes of woody savanna/ woodland and savanna/wooded grassland.

Quorum quenching is an antivirulence strategy employed by endophytic bacteria.

PubMed

Kusari, Parijat; Kusari, Souvik; Lamshöft, Marc; Sezgin, Selahaddin; Spiteller, Michael; Kayser, Oliver

2014-08-01

Bacteria predominantly use quorum sensing to regulate a plethora of physiological activities such as cell-cell crosstalk, mutualism, virulence, competence, biofilm formation, and antibiotic resistance. In this study, we investigated how certain potent endophytic bacteria harbored in Cannabis sativa L. plants use quorum quenching as an antivirulence strategy to disrupt the cell-to-cell quorum sensing signals in the biosensor strain, Chromobacterium violaceum. We used a combination of high-performance liquid chromatography high-resolution mass spectrometry (HPLC-ESI-HRMS(n)) and matrix-assisted laser desorption ionization imaging high-resolution mass spectrometry (MALDI-imaging-HRMS) to first quantify and visualize the spatial distribution of the quorum sensing molecules in the biosensor strain, C. violaceum. We then showed, both quantitatively and visually in high spatial resolution, how selected endophytic bacteria of C. sativa can selectively and differentially quench the quorum sensing molecules of C. violaceum. This study provides fundamental insights into the antivirulence strategies used by endophytes in order to survive in their ecological niches. Such defense mechanisms are evolved in order to thwart the plethora of pathogens invading associated host plants in a manner that prevents the pathogens from developing resistance against the plant/endophyte bioactive secondary metabolites. This work also provides evidence towards utilizing endophytes as tools for biological control of bacterial phytopathogens. In continuation, such insights would even afford new concepts and strategies in the future for combating drug resistant bacteria by quorum-inhibiting clinical therapies.

Predicting nucleic acid binding interfaces from structural models of proteins

PubMed Central

Dror, Iris; Shazman, Shula; Mukherjee, Srayanta; Zhang, Yang; Glaser, Fabian; Mandel-Gutfreund, Yael

2011-01-01

The function of DNA- and RNA-binding proteins can be inferred from the characterization and accurate prediction of their binding interfaces. However the main pitfall of various structure-based methods for predicting nucleic acid binding function is that they are all limited to a relatively small number of proteins for which high-resolution three dimensional structures are available. In this study, we developed a pipeline for extracting functional electrostatic patches from surfaces of protein structural models, obtained using the I-TASSER protein structure predictor. The largest positive patches are extracted from the protein surface using the patchfinder algorithm. We show that functional electrostatic patches extracted from an ensemble of structural models highly overlap the patches extracted from high-resolution structures. Furthermore, by testing our pipeline on a set of 55 known nucleic acid binding proteins for which I-TASSER produces high-quality models, we show that the method accurately identifies the nucleic acids binding interface on structural models of proteins. Employing a combined patch approach we show that patches extracted from an ensemble of models better predicts the real nucleic acid binding interfaces compared to patches extracted from independent models. Overall, these results suggest that combining information from a collection of low-resolution structural models could be a valuable approach for functional annotation. We suggest that our method will be further applicable for predicting other functional surfaces of proteins with unknown structure. PMID:22086767

Purification and high-resolution top-down mass spectrometric characterization of human salivary α-amylase.

PubMed

Peng, Ying; Chen, Xin; Sato, Takuya; Rankin, Scott A; Tsuji, Ryohei F; Ge, Ying

2012-04-03

Human salivary α-amylase (HSAMY) is a major component of salivary secretions, possessing multiple important biological functions. Here we have established three methods to purify HSAMY in human saliva for comprehensive characterization of HSAMY by high-resolution top-down mass spectrometry (MS). Among the three purification methods, the affinity method based on the enzyme-substrate specific interaction between amylase and glycogen is preferred, providing the highest purity HSAMY with high reproducibility. Subsequently, we employed Fourier transform ion cyclotron resonance MS to analyze the purified HSAMY. The predominant form of α-amylase purified from saliva of various races and genders is nonglycosylated with the same molecular weight of 55,881.2, which is 1885.8 lower than the calculated value based on the DNA-predicted sequence. High-resolution MS revealed the truncation of the first 15 N-terminal amino acids (-1858.96) and the subsequent formation of pyroglutamic acid at the new N-terminus Gln (-17.03). More importantly, five disulfide bonds in HSAMY were identified (-10.08) and effectively localized by tandem MS in conjunction with complete and partial reduction by tris (2-carboxyethyl) phosphine. Overall, this study demonstrates that top-down MS combined with affinity purification and partial reduction is a powerful method for rapid purification and complete characterization of large proteins with complex and overlapping disulfide bond patterns.

Hybrid system for in vivo real-time planar fluorescence and volumetric optoacoustic imaging

NASA Astrophysics Data System (ADS)

Chen, Zhenyue; Deán-Ben, Xosé Luís.; Gottschalk, Sven; Razansky, Daniel

2018-02-01

Fluorescence imaging is widely employed in all fields of cell and molecular biology due to its high sensitivity, high contrast and ease of implementation. However, the low spatial resolution and lack of depth information, especially in strongly-scattering samples, restrict its applicability for deep-tissue imaging applications. On the other hand, optoacoustic imaging is known to deliver a unique set of capabilities such as high spatial and temporal resolution in three dimensions, deep penetration and spectrally-enriched imaging contrast. Since fluorescent substances can generate contrast in both modalities, simultaneous fluorescence and optoacoustic readings can provide new capabilities for functional and molecular imaging of living organisms. Optoacoustic images can further serve as valuable anatomical references based on endogenous hemoglobin contrast. Herein, we propose a hybrid system for in vivo real-time planar fluorescence and volumetric optoacoustic tomography, both operating in reflection mode, which synergistically combines the advantages of stand-alone systems. Validation of the spatial resolution and sensitivity of the system were first carried out in tissue mimicking phantoms while in vivo imaging was further demonstrated by tracking perfusion of an optical contrast agent in a mouse brain in the hybrid imaging mode. Experimental results show that the proposed system effectively exploits the contrast mechanisms of both imaging modalities, making it especially useful for accurate monitoring of fluorescence-based signal dynamics in highly scattering samples.

20 CFR 411.620 - How long does the PM have to recommend a resolution to the dispute?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false How long does the PM have to recommend a resolution to the dispute? 411.620 Section 411.620 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE... Beneficiaries and Employment Networks § 411.620 How long does the PM have to recommend a resolution to the...

20 CFR 411.620 - How long does the PM have to recommend a resolution to the dispute?

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false How long does the PM have to recommend a resolution to the dispute? 411.620 Section 411.620 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE... Beneficiaries and Employment Networks § 411.620 How long does the PM have to recommend a resolution to the...

20 CFR 411.620 - How long does the PM have to recommend a resolution to the dispute?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false How long does the PM have to recommend a resolution to the dispute? 411.620 Section 411.620 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE... Beneficiaries and Employment Networks § 411.620 How long does the PM have to recommend a resolution to the...

20 CFR 411.620 - How long does the PM have to recommend a resolution to the dispute?

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false How long does the PM have to recommend a resolution to the dispute? 411.620 Section 411.620 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE... Beneficiaries and Employment Networks § 411.620 How long does the PM have to recommend a resolution to the...

20 CFR 411.620 - How long does the PM have to recommend a resolution to the dispute?

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false How long does the PM have to recommend a resolution to the dispute? 411.620 Section 411.620 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE... Beneficiaries and Employment Networks § 411.620 How long does the PM have to recommend a resolution to the...

Rainfall estimation from microwave links in São Paulo, Brazil.

NASA Astrophysics Data System (ADS)

Rios Gaona, Manuel Felipe; Overeem, Aart; Leijnse, Hidde; Uijlenhoet, Remko

2017-04-01

Rainfall estimation from microwave link networks has been successfully demonstrated in countries such as the Netherlands, Israel and Germany. The path-averaged rainfall intensity can be computed from the signal attenuation between cell phone towers. Although this technique is still in development, it offers great opportunities to retrieve rainfall rates at high spatiotemporal resolutions very close to the ground surface. High spatiotemporal resolutions and closer-to-ground measurements are highly appreciated, especially in urban catchments where high-impact events such as flash-floods develop in short time scales. We evaluate here this rainfall measurement technique for a tropical climate, something that has hardly been done previously. This is highly relevant since many countries with few surface rainfall observations are located in the tropics. The test-bed is the Brazilian city of São Paulo. The performance of 16 microwave links was evaluated, from a network of 200 links, for the last 3 months of 2014. The open software package RAINLINK was employed to obtain link rainfall estimates. The evaluation was done through a dense automatic gauge network. Results are promising and encouraging, especially for short links for which a high correlation (> 0.9) and a low bias (< 5%) were obtained.

Exploring the Fundamental of Fatigue in Composites: Opportunities using X-Ray Computed Tomography Imaging

DTIC Science & Technology

2012-10-01

matrix composite materials are employed in aerospace applications [1] and increasingly in other sectors such as sustainable energy (e.g. wind turbines ... ring . The difference in terms of source has important advantages for synchrotron radiation CT. One is represented by high spatial resolution...emery cloth between the specimen faces and the grip jaws (grit side toward specimen) was placed to avoid or reduce slip of the specimen in the grips

Response to comment on "High-resolution global maps of 21st-century forest cover change".

PubMed

Hansen, M; Potapov, P; Margono, B; Stehman, S; Turubanova, S; Tyukavina, A

2014-05-30

Tropek et al. critique the Hansen et al. global forest loss paper in terms of its utility and accuracy. Both criticisms suffer from a miscomprehension of the definition of forest employed as well as the requirements of product validation. Utility of the product is enhanced through its integration with forest type, carbon stock, protected area status, and other ancillary data. Copyright © 2014, American Association for the Advancement of Science.

An improved gravity model for Mars: Goddard Mars Model 1

NASA Technical Reports Server (NTRS)

Smith, D. E.; Lerch, F. J.; Nerem, R. S.; Zuber, M. T.; Patel, G. B.; Fricke, S. K.; Lemoine, F. G.

1993-01-01

Doppler tracking data of three orbiting spacecraft have been reanalyzed to develop a new gravitational field model for the planet Mars, Goddard Mars Model 1 (GMM-1). This model employs nearly all available data, consisting of approximately 1100 days of S band tracking data collected by NASA's Deep Space Network from the Mariner 9 and Viking 1 and Viking 2 spacecraft, in seven different orbits, between 1971 and 1979. GMM-1 is complete to spherical harmonic degree and order 50, which corresponds to a half-wavelength spatial resolution of 200-300 km where the data permit. GMM-1 represents satellite orbits with considerably better accuracy than previous Mars gravity models and shows greater resolution of identifiable geological structures. The notable improvement in GMM-1 over previous models is a consequence of several factors: improved computational capabilities, the use of otpimum weighting and least squares collocation solution techniques which stabilized the behavior of the solution at high degree and order, and the use of longer satellite arcs than employed in previous solutions that were made possible by improved force and measurement models. The inclusion of X band tracking data from the 379-km altitude, nnear-polar orbiting Mars Observer spacecraft should provide a significant improvement over GMM-1, particularly at high latitudes where current data poorly resolve the gravitational signature of the planet.

Cobalt internal standard for Ni to assist the simultaneous determination of Mo and Ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry employing direct solid sample analysis.

PubMed

de Babos, Diego Victor; Bechlin, Marcos André; Barros, Ariane Isis; Ferreira, Edilene Cristina; Gomes Neto, José Anchieta; de Oliveira, Silvana Ruella

2016-05-15

A new method is proposed for the simultaneous determination of Mo and Ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GFAAS), employing direct solid sample analysis (DSS) and internal standardization (IS). Cobalt was used as internal standard to minimize matrix effects during Ni determinations, enabling the use of aqueous standards for calibration. Correlation coefficients for the calibration curves were typically better than 0.9937. The performance of the method was checked by analysis of six plant certified reference materials, and the results for Mo and Ni were in agreement with the certified values (95% confidence level, t-test). Analysis was made of different types of plant materials used as renewable sources of energy, including sugarcane leaves, banana tree fiber, soybean straw, coffee pods, orange bagasse, peanut hulls, and sugarcane bagasse. The concentrations found for Mo and Ni ranged from 0.08 to 0.63 ng mg(-1) and from 0.41 to 6.92 ng mg(-1), respectively. Precision (RSD) varied from 2.1% to 11% for Mo and from 3.7% to 10% for Ni. Limits of quantification of 0.055 and 0.074 ng were obtained for Mo and Ni, respectively. Copyright © 2016 Elsevier B.V. All rights reserved.

An improved gravity model for Mars: Goddard Mars Model-1 (GMM-1)

NASA Technical Reports Server (NTRS)

Smith, D. E.; Lerch, F. J.; Nerem, R. S.; Zuber, M. T.; Patel, G. B.; Fricke, S. K.; Lemoine, F. G.

1993-01-01

Doppler tracking data of three orbiting spacecraft have been reanalyzed to develop a new gravitational field model for the planet Mars, GMM-1 (Goddard Mars Model-1). This model employs nearly all available data, consisting of approximately 1100 days of S-bank tracking data collected by NASA's Deep Space Network from the Mariner 9, and Viking 1 and Viking 2 spacecraft, in seven different orbits, between 1971 and 1979. GMM-1 is complete to spherical harmonic degree and order 50, which corresponds to a half-wavelength spatial resolution of 200-300 km where the data permit. GMM-1 represents satellite orbits with considerably better accuracy than previous Mars gravity models and shows greater resolution of identifiable geological structures. The notable improvement in GMM-1 over previous models is a consequence of several factors: improved computational capabilities, the use of optimum weighting and least-squares collocation solution techniques which stabilized the behavior of the solution at high degree and order, and the use of longer satellite arcs than employed in previous solutions that were made possible by improved force and measurement models. The inclusion of X-band tracking data from the 379-km altitude, near-polar orbiting Mars Observer spacecraft should provide a significant improvement over GMM-1, particularly at high latitudes where current data poorly resolves the gravitational signature of the planet.

Display system employing acousto-optic tunable filter

NASA Technical Reports Server (NTRS)

Lambert, James L. (Inventor)

1995-01-01

An acousto-optic tunable filter (AOTF) is employed to generate a display by driving the AOTF with a RF electrical signal comprising modulated red, green, and blue video scan line signals and scanning the AOTF with a linearly polarized, pulsed light beam, resulting in encoding of color video columns (scan lines) of an input video image into vertical columns of the AOTF output beam. The AOTF is illuminated periodically as each acoustically-encoded scan line fills the cell aperture of the AOTF. A polarizing beam splitter removes the unused first order beam component of the AOTF output and, if desired, overlays a real world scene on the output plane. Resolutions as high as 30,000 lines are possible, providing holographic display capability.

Display system employing acousto-optic tunable filter

NASA Technical Reports Server (NTRS)

Lambert, James L. (Inventor)

1993-01-01

An acousto-optic tunable filter (AOTF) is employed to generate a display by driving the AOTF with a RF electrical signal comprising modulated red, green, and blue video scan line signals and scanning the AOTF with a linearly polarized, pulsed light beam, resulting in encoding of color video columns (scan lines) of an input video image into vertical columns of the AOTF output beam. The AOTF is illuminated periodically as each acoustically-encoded scan line fills the cell aperture of the AOTF. A polarizing beam splitter removes the unused first order beam component of the AOTF output and, if desired, overlays a real world scene on the output plane. Resolutions as high as 30,000 lines are possible, providing holographic display capability.

The Mast Cameras and Mars Descent Imager (MARDI) for the 2009 Mars Science Laboratory

NASA Technical Reports Server (NTRS)

Malin, M. C.; Bell, J. F.; Cameron, J.; Dietrich, W. E.; Edgett, K. S.; Hallet, B.; Herkenhoff, K. E.; Lemmon, M. T.; Parker, T. J.; Sullivan, R. J.

2005-01-01

Based on operational experience gained during the Mars Exploration Rover (MER) mission, we proposed and were selected to conduct two related imaging experiments: (1) an investigation of the geology and short-term atmospheric vertical wind profile local to the Mars Science Laboratory (MSL) landing site using descent imaging, and (2) a broadly-based scientific investigation of the MSL locale employing visible and very near infra-red imaging techniques from a pair of mast-mounted, high resolution cameras. Both instruments share a common electronics design, a design also employed for the MSL Mars Hand Lens Imager (MAHLI) [1]. The primary differences between the cameras are in the nature and number of mechanisms and specific optics tailored to each camera s requirements.

Relative humidity vertical profiling using lidar-based synergistic methods in the framework of the Hygra-CD campaign

NASA Astrophysics Data System (ADS)

Labzovskii, Lev D.; Papayannis, Alexandros; Binietoglou, Ioannis; Banks, Robert F.; Baldasano, Jose M.; Toanca, Florica; Tzanis, Chris G.; Christodoulakis, John

2018-02-01

Accurate continuous measurements of relative humidity (RH) vertical profiles in the lower troposphere have become a significant scientific challenge. In recent years a synergy of various ground-based remote sensing instruments have been successfully used for RH vertical profiling, which has resulted in the improvement of spatial resolution and, in some cases, of the accuracy of the measurement. Some studies have also suggested the use of high-resolution model simulations as input datasets into RH vertical profiling techniques. In this paper we apply two synergetic methods for RH profiling, including the synergy of lidar with a microwave radiometer and high-resolution atmospheric modeling. The two methods are employed for RH retrieval between 100 and 6000 m with increased spatial resolution, based on datasets from the HygrA-CD (Hygroscopic Aerosols to Cloud Droplets) campaign conducted in Athens, Greece from May to June 2014. RH profiles from synergetic methods are then compared with those retrieved using single instruments or as simulated by high-resolution models. Our proposed technique for RH profiling provides improved statistical agreement with reference to radiosoundings by 27 % when the lidar-radiometer (in comparison with radiometer measurements) approach is used and by 15 % when a lidar model is used (in comparison with WRF-model simulations). Mean uncertainty of RH due to temperature bias in RH profiling was ˜ 4.34 % for the lidar-radiometer and ˜ 1.22 % for the lidar-model methods. However, maximum uncertainty in RH retrievals due to temperature bias showed that lidar-model method is more reliable at heights greater than 2000 m. Overall, our results have demonstrated the capability of both combined methods for daytime measurements in heights between 100 and 6000 m when lidar-radiometer or lidar-WRF combined datasets are available.

Whole-brain high in-plane resolution fMRI using accelerated EPIK for enhanced characterisation of functional areas at 3T

PubMed Central

Yun, Seong Dae

2017-01-01

The relatively high imaging speed of EPI has led to its widespread use in dynamic MRI studies such as functional MRI. An approach to improve the performance of EPI, EPI with Keyhole (EPIK), has been previously presented and its use in fMRI was verified at 1.5T as well as 3T. The method has been proven to achieve a higher temporal resolution and smaller image distortions when compared to single-shot EPI. Furthermore, the performance of EPIK in the detection of functional signals was shown to be comparable to that of EPI. For these reasons, we were motivated to employ EPIK here for high-resolution imaging. The method was optimised to offer the highest possible in-plane resolution and slice coverage under the given imaging constraints: fixed TR/TE, FOV and acceleration factors for parallel imaging and partial Fourier techniques. The performance of EPIK was evaluated in direct comparison to the optimised protocol obtained from EPI. The two imaging methods were applied to visual fMRI experiments involving sixteen subjects. The results showed that enhanced spatial resolution with a whole-brain coverage was achieved by EPIK (1.00 mm × 1.00 mm; 32 slices) when compared to EPI (1.25 mm × 1.25 mm; 28 slices). As a consequence, enhanced characterisation of functional areas has been demonstrated in EPIK particularly for relatively small brain regions such as the lateral geniculate nucleus (LGN) and superior colliculus (SC); overall, a significantly increased t-value and activation area were observed from EPIK data. Lastly, the use of EPIK for fMRI was validated with the simulation of different types of data reconstruction methods. PMID:28945780

A general CFD framework for fault-resilient simulations based on multi-resolution information fusion

NASA Astrophysics Data System (ADS)

Lee, Seungjoon; Kevrekidis, Ioannis G.; Karniadakis, George Em

2017-10-01

We develop a general CFD framework for multi-resolution simulations to target multiscale problems but also resilience in exascale simulations, where faulty processors may lead to gappy, in space-time, simulated fields. We combine approximation theory and domain decomposition together with statistical learning techniques, e.g. coKriging, to estimate boundary conditions and minimize communications by performing independent parallel runs. To demonstrate this new simulation approach, we consider two benchmark problems. First, we solve the heat equation (a) on a small number of spatial "patches" distributed across the domain, simulated by finite differences at fine resolution and (b) on the entire domain simulated at very low resolution, thus fusing multi-resolution models to obtain the final answer. Second, we simulate the flow in a lid-driven cavity in an analogous fashion, by fusing finite difference solutions obtained with fine and low resolution assuming gappy data sets. We investigate the influence of various parameters for this framework, including the correlation kernel, the size of a buffer employed in estimating boundary conditions, the coarseness of the resolution of auxiliary data, and the communication frequency across different patches in fusing the information at different resolution levels. In addition to its robustness and resilience, the new framework can be employed to generalize previous multiscale approaches involving heterogeneous discretizations or even fundamentally different flow descriptions, e.g. in continuum-atomistic simulations.

Fiber Fabry-Perot interferometer sensor for measuring resonances of piezoelectric elements

NASA Astrophysics Data System (ADS)

da Silva, Ricardo E.; Oliveira, Roberson A.; Pohl, Alexandre A. P.

2011-05-01

The development of a fiber extrinsic Fabry-Perot interferometer for measuring vibration amplitude and resonances of piezoelectric elements is reported. The signal demodulation method based on the use of an optical spectrum analyzer allows the measurement of displacements and resonances with high resolution. The technique consists basically in monitoring changes in the intensity or the wavelength of a single interferometric fringe at a point of high sensitivity in the sensor response curve. For sensor calibration, three signal processing techniques were employed. Vibration amplitude measurement with 0.84 nm/V sensitivity and the characterization of the piezo resonance is demonstrated.

Multidimensional Attosecond Resonant X-Ray Spectroscopy of Molecules: Lessons from the Optical Regime

PubMed Central

Mukamel, Shaul; Healion, Daniel; Zhang, Yu; Biggs, Jason D.

2013-01-01

New free-electron laser and high-harmonic generation X-ray light sources are capable of supplying pulses short and intense enough to perform resonant nonlinear time-resolved experiments in molecules. Valence-electron motions can be triggered impulsively by core excitations and monitored with high temporal and spatial resolution. We discuss possible experiments that employ attosecond X-ray pulses to probe the quantum coherence and correlations of valence electrons and holes, rather than the charge density alone, building on the analogy with existing studies of vibrational motions using femtosecond techniques in the visible regime. PMID:23245522

Development and characterization of non-resonant multiphoton photoacoustic spectroscopy (NMPPAS) for brain tumor margining

NASA Astrophysics Data System (ADS)

Dahal, Sudhir

During tumor removal surgery, due to the problems associated with obtaining high-resolution, real-time chemical images of where exactly the tumor ends and healthy tissue begins (tumor margining), it is often necessary to remove a much larger volume of tissue than the tumor itself. In the case of brain tumor surgery, however, it is extremely unsafe to remove excess tissue. Therefore, without an accurate image of the tumor margins, some of the tumor's finger-like projections are inevitably left behind in the surrounding parenchyma to grow again. For this reason, the development of techniques capable of providing high-resolution real-time images of tumor margins up to centimeters below the surface of a tissue is ideal for the diagnosis and treatment of tumors, as well as surgical guidance during brain tumor excision. A novel spectroscopic technique, non-resonant multiphoton photoacoustic spectroscopy (NMPPAS), is being developed with the capabilities of obtaining high-resolution subsurface chemical-based images of underlying tumors. This novel technique combines the strengths of multiphoton tissue spectroscopy and photoacoustic spectroscopy into a diagnostic methodology that will, ultimately, provide unparalleled chemical information and images to provide the state of sub-surface tissues. The NMPPAS technique employs near-infrared light (in the diagnostic window) to excite ultraviolet and/or visible light absorbing species deep below the tissue's surface. Once a multiphoton absorption event occurs, non-radiative relaxation processes generates a localized thermal expansion and subsequent acoustic wave that can be detected using a piezoelectric transducer. Since NMPPAS employs an acoustic detection modality, much deeper diagnoses can be performed than that is possible using current state of the art high-resolution chemical imaging techniques such as multiphoton fluorescence spectroscopy. NMPPAS was employed to differentiate between excised brain tumors (astrocytoma III) and healthy tissue with over 99% accuracy. NMPPAS spectral features showed evident differences between tumor and healthy tissues, and ratiometric analysis ensured that only a few wavelengths could be used for excitation instead of using numerous wavelength excitations to create spectra. This process would significantly reduce the analysis time while maintaining the same degree of accuracy. Tissue phantoms were fabricated in order to characterize the properties of NMPPAS. Scattering particles were doped into the phantoms to simulate their light scattering properties to real tissues. This allowed for better control over shape, size, reproducibility and doping in the sample while maintaining the light-tissue interaction properties of real tissue. To make NMPPAS viable for clinical applications, the technique was characterized to determine the spatial (lateral and longitudinal) resolution, depth of penetration and its ability to image in three-dimension through layers of tissue. Both resolutions were determined to be near-cellular level resolution (50-70 microm), obtained initially with the aid of the technique of multiphoton fluorescence, and later verified using NMPPAS imaging. Additionally, the maximum depth of penetration and detection was determined to be about 1.4cm, making the technique extremely suitable to margin tumors from underlying tissues in the brain. The capability of NMPPAS to detect and image layers that lie beneath other structures and blood vessels was also investigated. Three-dimensional images were obtained for the first time using NMPPAS. The images were obtained from different depths and structures were imaged through other layers of existing structures in the sample. This verified that NMPPAS was capable of detecting and imaging structures that lie embedded within the tissues. NMPPAS images of embedded structures were also obtained with the presence of hemoglobin, which is potentially the largest source of background in blood-perfused tissues, thus showing that the technique is capable of detecting and differentiating in blood-perfused samples.

Fabrication of high-resolution nanostructures of complex geometry by the single-spot nanolithography method

PubMed Central

Anisimova, Margarita; Samardak, Aleksei; Ognev, Alexey

2015-01-01

Summary The paper presents a method for the high-resolution production of polymer nanopatterns with controllable geometrical parameters by means of a single-spot electron-beam lithography technique. The essence of the method entails the overexposure of a positive-tone resist, spin-coated onto a substrate where nanoscale spots are exposed to an electron beam with a dose greater than 0.1 pC per dot. A single-spot enables the fabrication of a nanoring, while a chain of spots placed at distance of 5–30 nm from each other allows the production of a polymer pattern of complex geometry of sub-10 nm resolution. We demonstrate that in addition to the naturally oxidized silicon substrates, gold-coated substrates can also successfully be used for the single-spot nanopattering technique. An explanation of the results related to the resist overexposure was demonstrated using Monte Carlo simulations. Our nanofabrication method significantly accelerates (up to 10 times) the fabrication rate as compared to conventional lithography on positive-tone resist. This technique can be potentially employed in the electronics industry for the production of nanoprinted lithography molds, etching masks, nanoelectronics, nanophotonics, NEMS and MEMS devices. PMID:25977869

Low-cost and high-resolution interrogation scheme for LPG-based temperature sensor

NASA Astrophysics Data System (ADS)

Venkata Reddy, M.; Srimannarayana, K.; Venkatappa Rao, T.; Vengal Rao, P.

2015-09-01

A low-cost and high-resolution interrogation scheme for a long-period fiber grating (LPG) temperature sensor with adjustable temperature range has been designed, developed and tested. In general LPGs are widely used as optical sensors and can be used as optical edge filters to interrogate the wavelength encoded signal from sensors such as fiber Bragg grating (FBG) by converting it into intensity modulated signal. But the interrogation of LPG sensors using FBG is a bit novel and it is to be studied experimentally. The sensor works based on measurement of shift in attenuation band of LPG corresponding to the applied temperature. The wavelength shift of LPG attenuation band is monitored using an optical spectrum analyser (OSA). Further the bulk and expensive OSA is replaced with a low-cost interrogation system that employ an FBG, photodiode and a transimpedance amplifier (TIA). The designed interrogation scheme makes the system low-cost, fast in response, and also enhances its resolution up to 0.1°C. The measurable temperature range using the proposed scheme is limited to 120 °C. However this range can be shifted within 15-450 °C by means of adjusting the Bragg wavelength of FBG.

Note: Fully integrated time-to-amplitude converter in Si-Ge technology.

PubMed

Crotti, M; Rech, I; Ghioni, M

2010-10-01

Over the past years an always growing interest has arisen about the measurement technique of time-correlated single photon counting TCSPC), since it allows the analysis of extremely fast and weak light waveforms with a picoseconds resolution. Consequently, many applications exploiting TCSPC have been developed in several fields such as medicine and chemistry. Moreover, the development of multianode PMT and of single photon avalanche diode arrays led to the realization of acquisition systems with several parallel channels to employ the TCSPC technique in even more applications. Since TCSPC basically consists of the measurement of the arrival time of a photon, the most important part of an acquisition chain is the time measurement block, which must have high resolution and low differential nonlinearity, and in order to realize multidimensional systems, it has to be integrated to reduce both cost and area. In this paper we present a fully integrated time-to-amplitude converter, built in 0.35 μm Si-Ge technology, characterized by a good time resolution (60 ps), low differential nonlinearity (better than 3% peak to peak), high counting rate (16 MHz), low and constant power dissipation (40 mW), and low area occupation (1.38×1.28 mm(2)).

Fast, deep record length, time-resolved visible spectroscopy of plasmas using fiber grids

NASA Astrophysics Data System (ADS)

Brockington, Samuel; Case, Andrew; Cruz, Edward; Witherspoon, F. Douglas; Horton, Robert; Klauser, Ruth; Hwang, D. Q.

2016-10-01

HyperV Technologies is developing a fiber-coupled, deep-record-length, low-light camera head for performing high time resolution spectroscopy on visible emission from plasma events. New solid-state Silicon Photo-Multiplier (SiPM) chips are capable of single photon event detection and high speed data acquisition. By coupling the output of a spectrometer to an imaging fiber bundle connected to a bank of amplified SiPMs, time-resolved spectroscopic imagers of 100 to 1,000 pixels can be constructed. Target pixel performance is 10 Megaframes/sec with record lengths of up to 256,000 frames yielding 25.6 milliseconds of record at10 Megasamples/sec resolution. Pixel resolutions of 8 to 12 bits are pos- sible. Pixel pitch can be refined by using grids of 100 μm to 1000 μm diameter fibers. A prototype 32-pixel spectroscopic imager employing this technique was constructed and successfully tested at the University of California at Davis Compact Toroid Injection Experiment (CTIX) as a full demonstration of the concept. Experimental results will be dis-cussed, along with future plans for the Phase 2 project, and potential applications to plasma experiments . Work supported by USDOE SBIR Grant DE-SC0013801.

Analysis of fingerprints features of infrared spectra of various processed products of Radix Aconiti kusnezoffii

NASA Astrophysics Data System (ADS)

Tu-ya; Yang, Ping; Sun, Su-qin; Zhou, Qun; Bao, Xiao-hua; Noda, Isao

2010-06-01

Fourier-transform infrared spectroscopy (FTIR) and two-dimensional correlation infrared spectroscopy (2D-IR)) are employed to analyze various processed products and ether extracts of Radix Aconiti kusnezoffii. There is a resemblance among the spectra of different processed products. The major difference lies in the absorption peak at 1641 cm -1 in the IR spectra, which reflects the transformation of raw aconite to the processed products. There are distinctive differences in the absorption peaks in the range of 1800-1500 cm -1 in the second derivative spectra, which has better resolution, of different processed products. 2D-IR spectra, which elevate the resolution further, can present even more differences among the products in the range of 1800-800 cm -1. Analysis of ether extracts of various processed products proves that there are alcohols, esters, carboxylic acids or ketones in all of them. However, their contents in different samples have obvious differences. With the advantages of high resolution, high-speed and convenience, IR can quickly and precisely distinguish various processed products of Radix A. kusnezoffii, and can be applied to predict the tendency of transformation of the complicated chemical mixture systems under heat perturbation.

On the use of EEG or MEG brain imaging tools in neuromarketing research.

PubMed

Vecchiato, Giovanni; Astolfi, Laura; De Vico Fallani, Fabrizio; Toppi, Jlenia; Aloise, Fabio; Bez, Francesco; Wei, Daming; Kong, Wanzeng; Dai, Jounging; Cincotti, Febo; Mattia, Donatella; Babiloni, Fabio

2011-01-01

Here we present an overview of some published papers of interest for the marketing research employing electroencephalogram (EEG) and magnetoencephalogram (MEG) methods. The interest for these methodologies relies in their high-temporal resolution as opposed to the investigation of such problem with the functional Magnetic Resonance Imaging (fMRI) methodology, also largely used in the marketing research. In addition, EEG and MEG technologies have greatly improved their spatial resolution in the last decades with the introduction of advanced signal processing methodologies. By presenting data gathered through MEG and high resolution EEG we will show which kind of information it is possible to gather with these methodologies while the persons are watching marketing relevant stimuli. Such information will be related to the memorization and pleasantness related to such stimuli. We noted that temporal and frequency patterns of brain signals are able to provide possible descriptors conveying information about the cognitive and emotional processes in subjects observing commercial advertisements. These information could be unobtainable through common tools used in standard marketing research. We also show an example of how an EEG methodology could be used to analyze cultural differences between fruition of video commercials of carbonated beverages in Western and Eastern countries.

On the Use of EEG or MEG Brain Imaging Tools in Neuromarketing Research

PubMed Central

Vecchiato, Giovanni; Astolfi, Laura; De Vico Fallani, Fabrizio; Toppi, Jlenia; Aloise, Fabio; Bez, Francesco; Wei, Daming; Kong, Wanzeng; Dai, Jounging; Cincotti, Febo; Mattia, Donatella; Babiloni, Fabio

2011-01-01

Here we present an overview of some published papers of interest for the marketing research employing electroencephalogram (EEG) and magnetoencephalogram (MEG) methods. The interest for these methodologies relies in their high-temporal resolution as opposed to the investigation of such problem with the functional Magnetic Resonance Imaging (fMRI) methodology, also largely used in the marketing research. In addition, EEG and MEG technologies have greatly improved their spatial resolution in the last decades with the introduction of advanced signal processing methodologies. By presenting data gathered through MEG and high resolution EEG we will show which kind of information it is possible to gather with these methodologies while the persons are watching marketing relevant stimuli. Such information will be related to the memorization and pleasantness related to such stimuli. We noted that temporal and frequency patterns of brain signals are able to provide possible descriptors conveying information about the cognitive and emotional processes in subjects observing commercial advertisements. These information could be unobtainable through common tools used in standard marketing research. We also show an example of how an EEG methodology could be used to analyze cultural differences between fruition of video commercials of carbonated beverages in Western and Eastern countries. PMID:21960996

Brillouin Optical Correlation Domain Analysis in Composite Material Beams

PubMed Central

Stern, Yonatan; London, Yosef; Preter, Eyal; Antman, Yair; Diamandi, Hilel Hagai; Silbiger, Maayan; Adler, Gadi; Shalev, Doron; Zadok, Avi

2017-01-01

Structural health monitoring is a critical requirement in many composites. Numerous monitoring strategies rely on measurements of temperature or strain (or both), however these are often restricted to point-sensing or to the coverage of small areas. Spatially-continuous data can be obtained with optical fiber sensors. In this work, we report high-resolution distributed Brillouin sensing over standard fibers that are embedded in composite structures. A phase-coded, Brillouin optical correlation domain analysis (B-OCDA) protocol was employed, with spatial resolution of 2 cm and sensitivity of 1 °K or 20 micro-strain. A portable measurement setup was designed and assembled on the premises of a composite structures manufacturer. The setup was successfully utilized in several structural health monitoring scenarios: (a) monitoring the production and curing of a composite beam over 60 h; (b) estimating the stiffness and Young’s modulus of a composite beam; and (c) distributed strain measurements across the surfaces of a model wing of an unmanned aerial vehicle. The measurements are supported by the predictions of structural analysis calculations. The results illustrate the potential added values of high-resolution, distributed Brillouin sensing in the structural health monitoring of composites. PMID:28974041

In vivo functional photoacoustic microscopy of cutaneous microvasculature in human skin.

PubMed

Favazza, Christopher P; Cornelius, Lynn A; Wang, Lihong V

2011-02-01

Microcirculation is an important component of the cardiovascular system and can be used to assess systemic cardiovascular health. Numerous studies have investigated cutaneous microcirculation as an indicator of cardiovascular related diseases. Such research has shown promising results; however, there are many limitations regarding the employed measurement techniques, such as poor depth and spatial resolution and measurement versatility. Here we show the results of functional cutaneous microvascular experiments measured with photoacoustic microscopy, which provides high spatial resolution and multiparameter measurements. In a set of experiments, microvascular networks located in the palms of volunteers were perturbed by periodic ischemic events, and the subsequent hemodynamic response to the stimulus was recorded. Results indicate that during periods of arterial occlusion, the relative oxygen saturation of the capillary vessels decreased below resting levels, and temporarily increased above resting levels immediately following the occlusion. Furthermore, a hyperemic reaction to the occlusions was measured, and the observation agreed well with similar measurements using more conventional imaging techniques. Due to its exceptional capability to functionally image vascular networks with high spatial resolution, photoacoustic microscopy could be a beneficial biomedical tool to assess microvascular functioning and applied to patients with diseases that affect cardiovascular health. © 2011 Society of Photo-Optical Instrumentation Engineers.

Short range micro-power impulse radar with high resolution swept range gate with damped transmit and receive cavities

DOEpatents

McEwan, Thomas E.

1998-01-01

A radar range finder and hidden object locator is based on ultra-wide band radar with a high resolution swept range gate. The device generates an equivalent time amplitude scan with atypical range of 4 inches to 20 feet, and an analog range resolution as limited by a jitter of on the order of 0.01 inches. A differential sampling receiver is employed to effectively eliminate ringing and other aberrations induced in the receiver by the near proximity of the transmit antenna, so a background subtraction is not needed, simplifying the circuitry while improving performance. Uses of the invention include a replacement of ultrasound devices for fluid level sensing, automotive radar, such as cruise control and parking assistance, hidden object location, such as stud and rebar finding. Also, this technology can be used when positioned over a highway lane to collect vehicle count and speed data for traffic control. Techniques are used to reduce clutter in the receive signal, such as decoupling the receive and transmit cavities by placing a space between them, using conductive or radiative damping elements on the cavities, and using terminating plates on the sides of the openings.

Short range micro-power impulse radar with high resolution swept range gate with damped transmit and receive cavities

DOEpatents

McEwan, T.E.

1998-06-30

A radar range finder and hidden object locator is based on ultra-wide band radar with a high resolution swept range gate. The device generates an equivalent time amplitude scan with atypical range of 4 inches to 20 feet, and an analog range resolution as limited by a jitter of on the order of 0.01 inches. A differential sampling receiver is employed to effectively eliminate ringing and other aberrations induced in the receiver by the near proximity of the transmit antenna, so a background subtraction is not needed, simplifying the circuitry while improving performance. Uses of the invention include a replacement of ultrasound devices for fluid level sensing, automotive radar, such as cruise control and parking assistance, hidden object location, such as stud and rebar finding. Also, this technology can be used when positioned over a highway lane to collect vehicle count and speed data for traffic control. Techniques are used to reduce clutter in the receive signal, such as decoupling the receive and transmit cavities by placing a space between them, using conductive or radiative damping elements on the cavities, and using terminating plates on the sides of the openings. 20 figs.

Brillouin Optical Correlation Domain Analysis in Composite Material Beams.

PubMed

Stern, Yonatan; London, Yosef; Preter, Eyal; Antman, Yair; Diamandi, Hilel Hagai; Silbiger, Maayan; Adler, Gadi; Levenberg, Eyal; Shalev, Doron; Zadok, Avi

2017-10-02

Structural health monitoring is a critical requirement in many composites. Numerous monitoring strategies rely on measurements of temperature or strain (or both), however these are often restricted to point-sensing or to the coverage of small areas. Spatially-continuous data can be obtained with optical fiber sensors. In this work, we report high-resolution distributed Brillouin sensing over standard fibers that are embedded in composite structures. A phase-coded, Brillouin optical correlation domain analysis (B-OCDA) protocol was employed, with spatial resolution of 2 cm and sensitivity of 1 °K or 20 micro-strain. A portable measurement setup was designed and assembled on the premises of a composite structures manufacturer. The setup was successfully utilized in several structural health monitoring scenarios: (a) monitoring the production and curing of a composite beam over 60 h; (b) estimating the stiffness and Young's modulus of a composite beam; and (c) distributed strain measurements across the surfaces of a model wing of an unmanned aerial vehicle. The measurements are supported by the predictions of structural analysis calculations. The results illustrate the potential added values of high-resolution, distributed Brillouin sensing in the structural health monitoring of composites.

Simultaneous measurement of stratospheric O3, H2O, CH4, and N2O profiles from infrared limb thermal emissions

NASA Technical Reports Server (NTRS)

Abbas, M. M.; Glenn, M. J.; Kunde, V. G.; Brasunas, J.; Conrath, B. J.; Maguire, W. C.; Herman, J. R.

1987-01-01

Thermal emission measurements of the earth's stratospheric limb were made with a cryogenically cooled high-resolution Michelson interferometer on a balloon flight launched from Palestine, TX, on Nov. 6, 1984. Infrared spectra for complete limb sequences were obtained over portions of the 700-1940/cm range with an unapodized spectral resolution of 0.03/cm for tangent heights varying from 13 to 39 km. The observed data from 1125 to 1425/cm have been analyzed for simultaneous measurement of O3, H2O, CH4, and N2O profiles. The analysis employs line-by-line and layer-by-layer radiative-transfer calculations, including curvature and refraction effects. The optimum use of geometric and spectral effects is made to obtain sharply peaked weighting functions. Contributions from stratospheric aerosol are included by measuring the light extinction within the window regions of the observed spectra. The retrieved constituent profiles are compared with measurements made with a variety of techniques by other groups. The comparison shows good agreement with the published data for all gases, indicating the capability of retrieving trace gas profiles from high-resolution thermal emission limb measurements.

On the use of high-resolution topographic data as a proxy for seismic site conditions (VS30)

USGS Publications Warehouse

Allen, T.I.; Wald, D.J.

2009-01-01

An alternative method has recently been proposed for evaluating global seismic site conditions, or the average shear velocity to 30 m depth (VS30), from the Shuttle Radar Topography Mission (SRTM) 30 arcsec digital elevation models (DEMs). The basic premise of the method is that the topographic slope can be used as a reliable proxy for VS30 in the absence of geologically and geotechnically based site-condition maps through correlations between VS30 measurements and topographic gradient. Here we evaluate the use of higher-resolution (3 and 9 arcsec) DEMs to examine whether we are able to resolve VS30 in more detail than can be achieved using the lower-resolution SRTM data. High-quality DEMs at resolutions greater than 30 arcsec are not uniformly available at the global scale. However, in many regions where such data exist, they may be employed to resolve finer-scale variations in topographic gradient, and consequently, VS30. We use the U.S. Geological Survey Earth Resources Observation and Science (EROS) Data Center's National Elevation Dataset (NED) to investigate the use of high-resolution DEMs for estimating VS30 in several regions across the United States, including the San Francisco Bay area in California, Los Angeles, California, and St. Louis, Missouri. We compare these results with an example from Taipei, Taiwan, that uses 9 arcsec SRTM data, which are globally available. The use of higher-resolution NED data recovers finer-scale variations in topographic gradient, which better correlate to geological and geomorphic features, in particular, at the transition between hills and basins, warranting their use over 30 arcsec SRTM data where available. However, statistical analyses indicate little to no improvement over lower-resolution topography when compared to VS30 measurements, suggesting that some topographic smoothing may provide more stable VS30 estimates. Furthermore, we find that elevation variability in canopy-based SRTM measurements at resolutions greater than 30 arcsec are too large to resolve reliable slopes, particularly in low-gradient sedimentary basins.

A Holistic Framework for Deriving Equitable Apportionments and Resolving Conflicts in Transboundary Watercourses

NASA Astrophysics Data System (ADS)

Tyagi, H.; Gosain, A. K.; Khosa, R.

2017-12-01

Climate uncertainty & perpetually rising freshwater demands have emerged as the biggest threat to global water security. Consequently, water disputes have become more frequent & intense. If such conflicts remain unresolved for long, eventually they may cause severe socio-political damage to the riparians. The present study develops a comprehensive framework for conflict resolution & equitable allocation in transboundary Ganges watercourse with 4 stakeholder nations: China (Tibet), Nepal, India & Bangladesh. Scientific spatio-temporal information can be of great help in transboundary dispute resolution. Hence, this study employs a GIS-based semi-distributed SWAT hydrologic model for estimating water balance at different scales within the basin for present & future climate, landuse, storage & water use efficiency scenarios. The study analyses pertinent provisions of the Indian Constitution & examines the rulings of Indian water tribunals. It also critically compares various water dispute resolution mechanisms & doctrines on the barometer of equity & fairness to arrive at a procedurally & distributionally just water apportionment policy. The study makes use of Methods of Apportionments, Operations Research & Bankruptcy Rules to operationalize the chosen doctrine by devising an objective & quantifiable formulae for water allocation amongst the co-basin states for a range of flows. Furthermore, Game Theoretic and multi-optimization techniques have been used to rank the appropriateness of the above mentioned methods according to aggregate satisfaction/resentment of the stakeholders computed by equating their respective water claims with actual water shares obtained by them under different methods. Moreover, several Social Choice Theory methods have been employed to rate the performance of water allotment methods in a socio-political setting. The developed framework can thus be of great help for decision makers in effective water conflict resolution as transboundary basins cover nearly half of world's total land surface & there are high chances of water disputes in such transnational rivers.

TH-EF-BRA-11: Feasibility of Super-Resolution Time-Resolved 4DMRI for Multi-Breath Volumetric Motion Simulation in Radiotherapy Planning

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, G; Zakian, K; Deasy, J

Purpose: To develop a novel super-resolution time-resolved 4DMRI technique to evaluate multi-breath, irregular and complex organ motion without respiratory surrogate for radiotherapy planning. Methods: The super-resolution time-resolved (TR) 4DMRI approach combines a series of low-resolution 3D cine MRI images acquired during free breathing (FB) with a high-resolution breath-hold (BH) 3DMRI via deformable image registration (DIR). Five volunteers participated in the study under an IRB-approved protocol. The 3D cine images with voxel size of 5×5×5 mm{sup 3} at two volumes per second (2Hz) were acquired coronally using a T1 fast field echo sequence, half-scan (0.8) acceleration, and SENSE (3) parallel imaging.more » Phase-encoding was set in the lateral direction to minimize motion artifacts. The BH image with voxel size of 2×2×2 mm{sup 3} was acquired using the same sequence within 10 seconds. A demons-based DIR program was employed to produce super-resolution 2Hz 4DMRI. Registration quality was visually assessed using difference images between TR 4DMRI and 3D cine and quantitatively assessed using average voxel correlation. The fidelity of the 3D cine images was assessed using a gel phantom and a 1D motion platform by comparing mobile and static images. Results: Owing to voxel intensity similarity using the same MRI scanning sequence, accurate DIR between FB and BH images is achieved. The voxel correlations between 3D cine and TR 4DMRI are greater than 0.92 in all cases and the difference images illustrate minimal residual error with little systematic patterns. The 3D cine images of the mobile gel phantom preserve object geometry with minimal scanning artifacts. Conclusion: The super-resolution time-resolved 4DMRI technique has been achieved via DIR, providing a potential solution for multi-breath motion assessment. Accurate DIR mapping has been achieved to map high-resolution BH images to low-resolution FB images, producing 2Hz volumetric high-resolution 4DMRI. Further validation and improvement are still required prior to clinical applications. This study is in part supported by the NIH (U54CA137788/U54CA132378).« less

20 CFR 411.635 - Can a beneficiary be represented in the dispute resolution process under the Ticket to Work program?

Code of Federal Regulations, 2012 CFR

2012-04-01

... SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Ticket to Work Program Dispute Resolution Disputes Between Beneficiaries and Employment Networks § 411.635 Can a beneficiary be...