46 CFR 52.01-2 - Adoption of section I of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Adoption of section I of the ASME Boiler and Pressure Vessel Code. 52.01-2 Section 52.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General Requirements § 52.01-2 Adoption of section I of the ASME Boiler and Pressure Vessel Code. (a) Main power...

46 CFR 53.01-3 - Adoption of section IV of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Adoption of section IV of the ASME Boiler and Pressure Vessel Code. 53.01-3 Section 53.01-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING HEATING BOILERS General Requirements § 53.01-3 Adoption of section IV of the ASME Boiler and Pressure Vessel Code. (a) Heating...

Engineering and Design: Adsorption Design Guide

DTIC Science & Technology

2001-03-01

tested, and marked (or stamped) in accordance with the standards of the applicable Boiler and Pressure Vessel Code (ASME, 1992), and must incorporate...Boiler and Pressure Vessel Committee, Subcommittee on Pressure Vessels, 1992. ASME Boiler and Pressure Vessel Code , Section VIII, Rules for

A Comparison of Fatigue Design Methods

DTIC Science & Technology

2001-04-05

Boiler and Pressure Vessel Code does not...Engineers, "ASME Boiler and Pressure Vessel Code ," ASME, 3 Park Ave., New York, NY 10016-5990. [4] Langer, B. F., "Design of Pressure Vessels Involving... and Pressure Vessel Code [3] presents these methods and has expanded the procedures to other pressure vessels besides nuclear pressure vessels. B.

Effective Use of Weld Metal Yield Strength for HY-Steels

DTIC Science & Technology

1983-01-01

Boiler and Pressure Vessel Code The ASME Boiler and Pressure Vessel Code (B&PV Code) is divided...As noted earlier, the ASME Boiler and Pressure Vessel Code makes only one exception to its overall philosophy of matching weld-metal strength and...material where toughness is of primary importance. REFERENCES American Society of Mechanical Engineers, Boiler and Pressure Vessel

Preparing Technical Requirements for Third Party Contracting of Army Facilities

DTIC Science & Technology

1993-06-01

Boiler and Pressure Vessel Code Sec 9 Welding and Brazing Qualifications B 16.1 Cast Iron Pipe Flanges and Flanged...Control Terminology for Heating, Ventilating, Air Conditioning American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code B40.1...American National Standards Institute (ANSI) Boiler and Pressure Vessel Code (ASME) 125 Boilers and Pressure Vessels Code (ASTM) B31 Power

46 CFR 54.01-1 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

...://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. The material is also...) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) ASME Boiler and Pressure Vessel Code, Section VIII, Division 1, Rules for Construction of...

46 CFR 54.01-1 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

...://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. The material is also...) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) ASME Boiler and Pressure Vessel Code, Section VIII, Division 1, Rules for Construction of...

46 CFR 54.01-1 - Incorporation by reference.

Code of Federal Regulations, 2013 CFR

2013-10-01

...://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. The material is also... of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) ASME Boiler and Pressure Vessel Code, Section VIII, Division 1, Rules for Construction of Pressure Vessels...

46 CFR 54.01-1 - Incorporation by reference.

Code of Federal Regulations, 2014 CFR

2014-10-01

...://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. The material is also... of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) ASME Boiler and Pressure Vessel Code, Section VIII, Division 1, Rules for Construction of Pressure Vessels...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

Development of a Pebble-Bed Liquid-Nitrogen Evaporator and Superheater for the Scaled Large Blast/Thermal Simulator Facility

DTIC Science & Technology

1991-04-01

Boiler and Pressure Vessel Code . Other design requirements are developed from standard safe... Boiler and Pressure Vessel Code . The following three condi- tions constitute the primary design parameters for pressure vessels: (a) Design Working...rules and practices of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code . Section VIII, Division 1 of the ASME

ASME Code Efforts Supporting HTGRs

DOE Office of Scientific and Technical Information (OSTI.GOV)

D.K. Morton

2010-09-01

In 1999, an international collaborative initiative for the development of advanced (Generation IV) reactors was started. The idea behind this effort was to bring nuclear energy closer to the needs of sustainability, to increase proliferation resistance, and to support concepts able to produce energy (both electricity and process heat) at competitive costs. The U.S. Department of Energy has supported this effort by pursuing the development of the Next Generation Nuclear Plant, a high temperature gas-cooled reactor. This support has included research and development of pertinent data, initial regulatory discussions, and engineering support of various codes and standards development. This reportmore » discusses the various applicable American Society of Mechanical Engineers (ASME) codes and standards that are being developed to support these high temperature gascooled reactors during construction and operation. ASME is aggressively pursuing these codes and standards to support an international effort to build the next generation of advanced reactors so that all can benefit.« less

ASME Code Efforts Supporting HTGRs

DOE Office of Scientific and Technical Information (OSTI.GOV)

D.K. Morton

2011-09-01

In 1999, an international collaborative initiative for the development of advanced (Generation IV) reactors was started. The idea behind this effort was to bring nuclear energy closer to the needs of sustainability, to increase proliferation resistance, and to support concepts able to produce energy (both electricity and process heat) at competitive costs. The U.S. Department of Energy has supported this effort by pursuing the development of the Next Generation Nuclear Plant, a high temperature gas-cooled reactor. This support has included research and development of pertinent data, initial regulatory discussions, and engineering support of various codes and standards development. This reportmore » discusses the various applicable American Society of Mechanical Engineers (ASME) codes and standards that are being developed to support these high temperature gascooled reactors during construction and operation. ASME is aggressively pursuing these codes and standards to support an international effort to build the next generation of advanced reactors so that all can benefit.« less

ASME Code Efforts Supporting HTGRs

DOE Office of Scientific and Technical Information (OSTI.GOV)

D.K. Morton

2012-09-01

In 1999, an international collaborative initiative for the development of advanced (Generation IV) reactors was started. The idea behind this effort was to bring nuclear energy closer to the needs of sustainability, to increase proliferation resistance, and to support concepts able to produce energy (both electricity and process heat) at competitive costs. The U.S. Department of Energy has supported this effort by pursuing the development of the Next Generation Nuclear Plant, a high temperature gas-cooled reactor. This support has included research and development of pertinent data, initial regulatory discussions, and engineering support of various codes and standards development. This reportmore » discusses the various applicable American Society of Mechanical Engineers (ASME) codes and standards that are being developed to support these high temperature gascooled reactors during construction and operation. ASME is aggressively pursuing these codes and standards to support an international effort to build the next generation of advanced reactors so that all can benefit.« less

78 FR 37721 - Approval of American Society of Mechanical Engineers' Code Cases

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-24

...-0359] RIN 3150-AI72 Approval of American Society of Mechanical Engineers' Code Cases AGENCY: Nuclear... mandatory American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel (BPV) Code and... Guide'' series. In a notice of proposed rulemaking, ``Approval of American Society of Mechanical...

76 FR 36231 - American Society of Mechanical Engineers (ASME) Codes and New and Revised ASME Code Cases

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-21

... exceed the test facility limits and reduces the number of functional tests for specific valve designs... addresses reducing the number of functional tests for specific valve designs. The NRC has identified no... the required test pressure for the new Class 1 incompressible-fluid, pressure-relief valve designs...

77 FR 46385 - Certain Small Diameter Seamless Carbon and Alloy Standard, Line, and Pressure Pipe From Germany...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-03

...: Seamless pressure pipes are intended for the conveyance of water, steam, petrochemicals, chemicals, oil... Mechanical Engineers (ASME) code stress levels. Alloy pipes made to ASTM standard A-335 must be used if temperatures and stress levels exceed those allowed for A-106 and the ASME codes. Seamless pressure pipes sold...

Computer-Aided Thermohydraulic Design of TEMA Type E Shell and Tube Heat Exchangers for Use in Low Pressure, Liquid-to-Liquid, Single Phase Applications.

DTIC Science & Technology

1985-04-01

and Standards .. ... ....... ....... 9 A. General . ... .. .. ... ..... .. .. ... 9 B. ASME Boiler and Pressure Vessel Code .. .. ......9 C. Foreign...several different sources. B. American Society of Mechanial Engineers (ASME) Boiler and Pressure Vessel Code A shell and tube heat exchanger is indeed a

Globalization of ASME Nuclear Codes and Standards

DOE Office of Scientific and Technical Information (OSTI.GOV)

Swayne, Rick; Erler, Bryan A.

2006-07-01

With the globalization of the nuclear industry, it is clear that the reactor suppliers are based in many countries around the world (such as United States, France, Japan, Canada, South Korea, South Africa) and they will be marketing their reactors to many countries around the world (such as US, China, South Korea, France, Canada, Finland, Taiwan). They will also be fabricating their components in many different countries around the world. With this situation, it is clear that the requirements of ASME Nuclear Codes and Standards need to be adjusted to accommodate the regulations, fabricating processes, and technology of various countriesmore » around the world. It is also very important for the American Society of Mechanical Engineers (ASME) to be able to assure that products meeting the applicable ASME Code requirements will provide the same level of safety and quality assurance as those products currently fabricated under the ASME accreditation process. To do this, many countries are in the process of establishing or changing their regulations, and it is important for ASME to interface with the appropriate organizations in those countries, in order to ensure there is effective use of ASME Codes and standards around the world. (authors)« less

Development of a Pebble-Bed Liquid-Nitrogen Evaporator/Superheater for the BRL 1/6th Scale Large Blast/Thermal Simulator Test Bed. Phase 1. Prototype Design and Analysis

DTIC Science & Technology

1991-08-01

specifications are taken primarily from the 1983 version of the ASME Boiler and Pressure Vessel Code . Other design requirements were developea from standard safe...rules and practices of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code to provide a safe and reliable system

Fracture Analysis of Welded Type 304 Stainless Steel Pipe

DTIC Science & Technology

1986-11-01

American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code . In order to accomplish these objectives, a series of seven full...Mechanical Engineers Boiler and Pressure Vessel Code , Section XI IWB-3640 (Winter Addenda 1983). 5. Ranganath, S., and U.S. Mehta, "Engineering Methods for

A Generic Structural Integrity Assurance Technology Program for the Army

DTIC Science & Technology

1989-11-01

and Pressure Vessel Code , American Society of Mechanical Engineers, 1986. DEFINITIONS AND ACRONYMS Definitions A-Basis: At least 99 percent of the...Aluminum Bridge and Other Highway Structures, 1976. Aluminum Association Specifications for Aluminum Structures, Third Edition, 1976. ASME ASME Boiler

Accelerator System Model (ASM) user manual with physics and engineering model documentation. ASM version 1.0

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1993-07-01

The Accelerator System Model (ASM) is a computer program developed to model proton radiofrequency accelerators and to carry out system level trade studies. The ASM FORTRAN subroutines are incorporated into an intuitive graphical user interface which provides for the {open_quotes}construction{close_quotes} of the accelerator in a window on the computer screen. The interface is based on the Shell for Particle Accelerator Related Codes (SPARC) software technology written for the Macintosh operating system in the C programming language. This User Manual describes the operation and use of the ASM application within the SPARC interface. The Appendix provides a detailed description of themore » physics and engineering models used in ASM. ASM Version 1.0 is joint project of G. H. Gillespie Associates, Inc. and the Accelerator Technology (AT) Division of the Los Alamos National Laboratory. Neither the ASM Version 1.0 software nor this ASM Documentation may be reproduced without the expressed written consent of both the Los Alamos National Laboratory and G. H. Gillespie Associates, Inc.« less

40 CFR 62.15265 - How do I monitor the load of my municipal waste combustion unit?

Code of Federal Regulations, 2013 CFR

2013-07-01

... Mechanical Engineers (ASME PTC 4.1—1964): Test Code for Steam Generating Units, Power Test Code 4.1-1964... of Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You....archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. (4) Design, construct...

40 CFR 62.15265 - How do I monitor the load of my municipal waste combustion unit?

Code of Federal Regulations, 2011 CFR

2011-07-01

... Mechanical Engineers (ASME PTC 4.1—1964): Test Code for Steam Generating Units, Power Test Code 4.1-1964... of Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You....archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. (4) Design, construct...

40 CFR 62.15265 - How do I monitor the load of my municipal waste combustion unit?

Code of Federal Regulations, 2010 CFR

2010-07-01

... Mechanical Engineers (ASME PTC 4.1—1964): Test Code for Steam Generating Units, Power Test Code 4.1-1964... of Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You....archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. (4) Design, construct...

40 CFR 62.15265 - How do I monitor the load of my municipal waste combustion unit?

Code of Federal Regulations, 2012 CFR

2012-07-01

... Mechanical Engineers (ASME PTC 4.1—1964): Test Code for Steam Generating Units, Power Test Code 4.1-1964... of Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You....archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. (4) Design, construct...

40 CFR 62.15265 - How do I monitor the load of my municipal waste combustion unit?

Code of Federal Regulations, 2014 CFR

2014-07-01

... Mechanical Engineers (ASME PTC 4.1—1964): Test Code for Steam Generating Units, Power Test Code 4.1-1964... of Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You....archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html. (4) Design, construct...

The Effect of Weld Metal Strength Mismatch on the Deformation and Fracture Behavior of Steel Butt Weldments

DTIC Science & Technology

1991-01-01

Society 6 of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code [ 1980]. Their results are similar to those of Satoh and Toyoda, and are...E813-89. American Society of Mechanical Engineers, Boiler and Pressure Vessel Code , Section III, Nuclear Power Plant Components, 1980. American

46 CFR 162.018-1 - Applicable specifications, and referenced material.

Code of Federal Regulations, 2012 CFR

2012-10-01

...) The ASME Code may be obtained from the American Society of Mechanical Engineers, United Engineering..., CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL ENGINEERING EQUIPMENT Safety Relief Valves, Liquefied... (Procedures Applicable to the Public) and Subpart 50.15 of Subchapter F (Marine Engineering) of this chapter...

46 CFR 162.018-1 - Applicable specifications, and referenced material.

Code of Federal Regulations, 2013 CFR

2013-10-01

...) The ASME Code may be obtained from the American Society of Mechanical Engineers, United Engineering..., CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL ENGINEERING EQUIPMENT Safety Relief Valves, Liquefied... (Procedures Applicable to the Public) and Subpart 50.15 of Subchapter F (Marine Engineering) of this chapter...

46 CFR 162.018-1 - Applicable specifications, and referenced material.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) The ASME Code may be obtained from the American Society of Mechanical Engineers, United Engineering..., CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL ENGINEERING EQUIPMENT Safety Relief Valves, Liquefied... (Procedures Applicable to the Public) and Subpart 50.15 of Subchapter F (Marine Engineering) of this chapter...

46 CFR 162.018-1 - Applicable specifications, and referenced material.

Code of Federal Regulations, 2014 CFR

2014-10-01

...) The ASME Code may be obtained from the American Society of Mechanical Engineers, United Engineering..., CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL ENGINEERING EQUIPMENT Safety Relief Valves, Liquefied... (Procedures Applicable to the Public) and Subpart 50.15 of Subchapter F (Marine Engineering) of this chapter...

46 CFR 162.018-1 - Applicable specifications, and referenced material.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) The ASME Code may be obtained from the American Society of Mechanical Engineers, United Engineering..., CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL ENGINEERING EQUIPMENT Safety Relief Valves, Liquefied... (Procedures Applicable to the Public) and Subpart 50.15 of Subchapter F (Marine Engineering) of this chapter...

29 CFR 1910.402 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Code or equivalent: ASME (American Society of Mechanical Engineers) Boiler and Pressure Vessel Code... pressure: The pressure at which a pressure containment device would fail structurally. Cylinder: A pressure vessel for the storage of gases. Decompression chamber: A pressure vessel for human occupancy such as a...

29 CFR 1910.402 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Code or equivalent: ASME (American Society of Mechanical Engineers) Boiler and Pressure Vessel Code... pressure: The pressure at which a pressure containment device would fail structurally. Cylinder: A pressure vessel for the storage of gases. Decompression chamber: A pressure vessel for human occupancy such as a...

29 CFR 1910.402 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Code or equivalent: ASME (American Society of Mechanical Engineers) Boiler and Pressure Vessel Code... pressure: The pressure at which a pressure containment device would fail structurally. Cylinder: A pressure vessel for the storage of gases. Decompression chamber: A pressure vessel for human occupancy such as a...

Potential advantages associated with implementing a risk-based inspection program by a nuclear facility

NASA Astrophysics Data System (ADS)

McNeill, Alexander, III; Balkey, Kenneth R.

1995-05-01

The current inservice inspection activities at a U.S. nuclear facility are based upon the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code, Section XI. The Code selects examination locations based upon a sampling criteria which includes component geometry, stress, and usage among other criteria. This can result in a significant number of required examinations. As a result of regulatory action each nuclear facility has conducted probabilistic risk assessments (PRA) or individual plant examinations (IPE), producing plant specific risk-based information. Several initiatives have been introduced to apply this new plant risk information. Among these initiatives is risk-based inservice inspection. A code case has been introduced for piping inspections based upon this new risk- based technology. This effort brought forward to the ASME Section XI Code committee, has been initiated and championed by the ASME Research Task Force on Risk-Based Inspection Guidelines -- LWR Nuclear Power Plant Application. Preliminary assessments associated with the code case have revealed that potential advantages exist in a risk-based inservice inspection program with regard to a number of exams, risk, personnel exposure, and cost.

Assuring Structural Integrity in Army Systems

DTIC Science & Technology

1985-02-28

power plants are* I. American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code , Section III - Rules for Construction of Nuclear...Power Plant Components; 2. ASNE Boiler and Pressure Vessel Code , Section XI, Rules for In-Service Inspection of Nuclear Power Plant Components; and 3

ASTM and ASME-BPE Standards--Complying with the Needs of the Pharmaceutical Industry.

PubMed

Huitt, William M

2011-01-01

Designing and building a pharmaceutical facility requires the owner, engineer of record, and constructor to be knowledgeable with regard to the industry codes and standards that apply to this effort. Up until 1997 there were no industry standards directed at the needs and requirements of the pharmaceutical industry. Prior to that time it was a patchwork effort at resourcing and adopting nonpharmaceutical-related codes and standards and then modifying them in order to meet the more stringent requirements of the Food and Drug Administration (FDA). In 1997 the American Society of Mechanical Engineers (ASME) published the first Bioprocessing Equipment (BPE) Standard. Through harmonization efforts this relatively new standard has brought together, scrutinized, and refined industry accepted methodologies together with FDA compliance requirements, and has established an American National Standard that provides a comprehensive set of standards that are integral to the pharmaceutical industry. This article describes various American National Standards, including those developed and published by the American Society for Testing and Materials (ASTM), and how they apply to the pharmaceutical industry. It goes on to discuss the harmonization effort that takes place between the various standards developers in an attempt to prevent conflicts and omissions between the many standards. Also included are examples of tables and figures taken from the ASME-BPE Standard. These examples provide the reader with insight to the relevant content of the ASME-BPE Standard. Designing and building a pharmaceutical facility requires the owner, engineer of record, and constructor to be knowledgeable with regard to the industry codes and standards that apply to this effort. Up until 1997 there were no industry standards directed at the needs and requirements of the pharmaceutical industry. Prior to that time it was a patchwork effort at resourcing and adopting nonpharmaceutical-related codes and standards and then modifying them in order to meet the more stringent requirements of the Food and Drug Administration (FDA). In 1997 the American Society of Mechanical Engineers (ASME) published the first Bioprocessing Equipment (BPE) Standard. In its initial development and ongoing maintenance it works with other American National Standards developers to harmonize the many standards associated with the design, engineering, and construction of bioprocessing facilities. This harmonization effort has established a comprehensive set of standards for the betterment of the pharmaceutical industry at large. This effort is, and will remain, very important as technology, along with new and improved product and processes, evolve into the future.

Development of Yield and Tensile Strength Design Curves for Alloy 617

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nancy Lybeck; T. -L. Sham

2013-10-01

The U.S. Department of Energy Very High Temperature Reactor Program is acquiring data in preparation for developing an Alloy 617 Code Case for inclusion in the nuclear section of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel (B&PV) Code. A draft code case was previously developed, but effort was suspended before acceptance by ASME. As part of the draft code case effort, a database was compiled of yield and tensile strength data from tests performed in air. Yield strength and tensile strength at temperature are used to set time independent allowable stress for construction materials in B&PVmore » Code, Section III, Subsection NH. The yield and tensile strength data used for the draft code case has been augmented with additional data generated by Idaho National Laboratory and Oak Ridge National Laboratory in the U.S. and CEA in France. The standard ASME Section II procedure for generating yield and tensile strength at temperature is presented, along with alternate methods that accommodate the change in temperature trends seen at high temperatures, resulting in a more consistent design margin over the temperature range of interest.« less

Gap Analysis of Material Properties Data for Ferritic/Martensitic HT-9 Steel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brown, Neil R.; Serrano De Caro, Magdalena; Rodriguez, Edward A.

2012-08-28

The US Department of Energy (DOE), Office of Nuclear Energy (NE), is supporting the development of an ASME Code Case for adoption of 12Cr-1Mo-VW ferritic/martensitic (F/M) steel, commonly known as HT-9, primarily for use in elevated temperature design of liquid-metal fast reactors (LMFR) and components. In 2011, Los Alamos National Laboratory (LANL) nuclear engineering staff began assisting in the development of a small modular reactor (SMR) design concept, previously known as the Hyperion Module, now called the Gen4 Module. LANL staff immediately proposed HT-9 for the reactor vessel and components, as well as fuel clad and ducting, due to itsmore » superior thermal qualities. Although the ASME material Code Case, for adoption of HT-9 as an approved elevated temperature material for LMFR service, is the ultimate goal of this project, there are several key deliverables that must first be successfully accomplished. The most important key deliverable is the research, accumulation, and documentation of specific material parameters; physical, mechanical, and environmental, which becomes the basis for an ASME Code Case. Time-independent tensile and ductility data and time-dependent creep and creep-rupture behavior are some of the material properties required for a successful ASME Code case. Although this report provides a cursory review of the available data, a much more comprehensive study of open-source data would be necessary. This report serves three purposes: (a) provides a list of already existing material data information that could ultimately be made available to the ASME Code, (b) determines the HT-9 material properties data missing from available sources that would be required and (c) estimates the necessary material testing required to close the gap. Ultimately, the gap analysis demonstrates that certain material properties testing will be required to fulfill the necessary information package for an ASME Code Case.« less

78 FR 37885 - Approval of American Society of Mechanical Engineers' Code Cases

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-24

...), standard design certifications, standard design approvals and manufacturing licenses, to use the Code Cases... by the ASME. The three RGs that would be incorporated by reference are RG 1.84, ``Design, Fabrication... nuclear power plant licensees, and applicants for CPs, OLs, COLs, standard design certifications, standard...

40 CFR 62.14104 - Requirements for municipal waste combustor operating practices.

Code of Federal Regulations, 2013 CFR

2013-07-01

...), proceed in accordance with ASME PTC 4.1-1964 (Reaffirmed 1991), Power Test Codes: Test Code for Steam Generating Units (with 1968 and 1969 Addenda). For design, construction, installation, calibration, and use... Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You may...

40 CFR 62.14104 - Requirements for municipal waste combustor operating practices.

Code of Federal Regulations, 2014 CFR

2014-07-01

...), proceed in accordance with ASME PTC 4.1-1964 (Reaffirmed 1991), Power Test Codes: Test Code for Steam Generating Units (with 1968 and 1969 Addenda). For design, construction, installation, calibration, and use... Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You may...

40 CFR 62.14104 - Requirements for municipal waste combustor operating practices.

Code of Federal Regulations, 2010 CFR

2010-07-01

...), proceed in accordance with ASME PTC 4.1-1964 (Reaffirmed 1991), Power Test Codes: Test Code for Steam Generating Units (with 1968 and 1969 Addenda). For design, construction, installation, calibration, and use... Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You may...

40 CFR 62.14104 - Requirements for municipal waste combustor operating practices.

Code of Federal Regulations, 2012 CFR

2012-07-01

...), proceed in accordance with ASME PTC 4.1-1964 (Reaffirmed 1991), Power Test Codes: Test Code for Steam Generating Units (with 1968 and 1969 Addenda). For design, construction, installation, calibration, and use... Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You may...

40 CFR 62.14104 - Requirements for municipal waste combustor operating practices.

Code of Federal Regulations, 2011 CFR

2011-07-01

...), proceed in accordance with ASME PTC 4.1-1964 (Reaffirmed 1991), Power Test Codes: Test Code for Steam Generating Units (with 1968 and 1969 Addenda). For design, construction, installation, calibration, and use... Mechanical Engineers, Service Center, 22 Law Drive, Post Office Box 2900, Fairfield, NJ 07007. You may...

Guidelines for the Design, Fabrication, Testing, Installation and Operation of Srf Cavities

NASA Astrophysics Data System (ADS)

Theilacker, J.; Carter, H.; Foley, M.; Hurh, P.; Klebaner, A.; Krempetz, K.; Nicol, T.; Olis, D.; Page, T.; Peterson, T.; Pfund, P.; Pushka, D.; Schmitt, R.; Wands, R.

2010-04-01

Superconducting Radio-Frequency (SRF) cavities containing cryogens under pressure pose a potential rupture hazard to equipment and personnel. Generally, pressure vessels fall within the scope of the ASME Boiler and Pressure Vessel Code however, the use of niobium as a material for the SRF cavities is beyond the applicability of the Code. Fermilab developed a guideline to ensure sound engineering practices governing the design, fabrication, testing, installation and operation of SRF cavities. The objective of the guideline is to reduce hazards and to achieve an equivalent level of safety afforded by the ASME Code. The guideline addresses concerns specific to SRF cavities in the areas of materials, design and analysis, welding and brazing, pressure relieving requirements, pressure testing and quality control.

Design and Analysis of Boiler Pressure Vessels based on IBR codes

NASA Astrophysics Data System (ADS)

Balakrishnan, B.; Kanimozhi, B.

2017-05-01

Pressure vessels components are widely used in the thermal and nuclear power plants for generating steam using the philosophy of heat transfer. In Thermal power plant, Coal is burnt inside the boiler furnace for generating the heat. The amount of heat produced through the combustion of pulverized coal is used in changing the phase transfer (i.e. Water into Super-Heated Steam) in the Pressure Parts Component. Pressure vessels are designed as per the Standards and Codes of the country, where the boiler is to be installed. One of the Standards followed in designing Pressure Parts is ASME (American Society of Mechanical Engineers). The mandatory requirements of ASME code must be satisfied by the manufacturer. In our project case, A Shell/pipe which has been manufactured using ASME code has an issue during the drilling of hole. The Actual Size of the drilled holes must be, as per the drawing, but due to error, the size has been differentiate from approved design calculation (i.e. the diameter size has been exceeded). In order to rectify this error, we have included an additional reinforcement pad to the drilled and modified the design of header in accordance with the code requirements.

75 FR 80765 - Hazardous Materials: Adoption of ASME Code Section XII and the National Board Inspection Code

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-23

... requirement for allowable peak secondary stresses for MC 331 cargo tanks. 4. Rational Design of Non-circular... the design, construction, and certification of cargo tank motor vehicles, cryogenic portable tanks and... CTMV: Cargo Tank Motor Vehicle DCE: Design Certifying Engineer FMCSA: Federal Motor Carrier Safety...

Central Heat Plant Modernization: FY98 Update and Recommendations.

DTIC Science & Technology

1999-12-01

Boiler and Pressure Vessel Code suggests an inspection frequency of 12 months for...28 April 1997). ASME International, Boiler and Pressure Vessel Code (ASME International, New York, NY, 1995). Bloomquist, R.G., J.D. Nimmons, and K...Services (HQDA, 28 April 1997). ASME International, Boiler and Pressure Vessel Code (ASME International, New York, NY, 1995). Bloomquist, R.G.,

76 FR 53497 - Florida Power and Light Company; St. Lucie Plant, Units 1 and 2; Environmental Assessment and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-26

... Appendix G to the Code for calculating K IM factors, and instead applies FEM [finite element modeling..., Units 1 and 2 are calculated using the CE NSSS finite element modeling methods. The Need for the... Society of Mechanical Engineers (ASME) Code, Section XI, Appendix G) or determined by applying finite...

49 CFR Appendix B to Part 222 - Alternative Safety Measures

Code of Federal Regulations, 2010 CFR

2010-10-01

...-Engineering ASMs, and Engineering ASMs. Modified SSMs are SSMs that do not fully comply with the provisions... reduction credit for pre-existing modified SSMs under the final rule. Non-engineering ASMs consist of... reduce risk within a quiet zone. Engineering ASMs consist of engineering improvements that address...

49 CFR Appendix B to Part 222 - Alternative Safety Measures

Code of Federal Regulations, 2011 CFR

2011-10-01

...-Engineering ASMs, and Engineering ASMs. Modified SSMs are SSMs that do not fully comply with the provisions... reduction credit for pre-existing modified SSMs under the final rule. Non-engineering ASMs consist of... reduce risk within a quiet zone. Engineering ASMs consist of engineering improvements that address...

PRA and Risk Informed Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bernsen, Sidney A.; Simonen, Fredric A.; Balkey, Kenneth R.

2006-01-01

The Boiler and Pressure Vessel Code (BPVC) of the American Society of Mechanical Engineers (ASME) has introduced a risk based approach into Section XI that covers Rules for Inservice Inspection of Nuclear Power Plant Components. The risk based approach requires application of the probabilistic risk assessments (PRA). Because no industry consensus standard existed for PRAs, ASME has developed a standard to evaluate the quality level of an available PRA needed to support a given risk based application. The paper describes the PRA standard, Section XI application of PRAs, and plans for broader applications of PRAs to other ASME nuclear codesmore » and standards. The paper addresses several specific topics of interest to Section XI. Important consideration are special methods (surrogate components) used to overcome the lack of PRA treatments of passive components in PRAs. The approach allows calculations of conditional core damage probabilities both for component failures that cause initiating events and failures in standby systems that decrease the availability of these systems. The paper relates the explicit risk based methods of the new Section XI code cases to the implicit consideration of risk used in the development of Section XI. Other topics include the needed interactions of ISI engineers, plant operating staff, PRA specialists, and members of expert panels that review the risk based programs.« less

Requirements for construction of nuclear system components at elevated temperatures (supplement to ASME Code Cases 1592, 1593, 1594, 1595, and 1596)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This standard provides rules for the construction of Class 1 nuclear components, parts, and appurtenances for use at elevated temperatures. This standard is a complete set of requirements only when used in conjunction with Section III of the ASME Boiler and Pressure Vessel Code (ASME Code) and addenda, ASME Code Cases 1592, 1593, 1594, 1595, and 1596, and RDT E 15-2NB. Unmodified paragraphs of the referenced Code Cases are not repeated in this standard but are a part of the requirements of this standard.

The Bauschinger Effect in Autofrettaged Tubes- A Comparison of Models Including the ASME Code

DTIC Science & Technology

1998-06-01

possible error in Division 3 of Section Vm of the ASME Boiler and Pressure Vessel Code . They show that the empirical method used in the code to...Discussion presented by DP Kendall We appreciate the acknowledgement in the Kendall discussion that Division 3 of Section VIII of the ASME Boiler and Pressure Vessel Code may

Pressure Safety Program Implementation at ORNL

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lower, Mark; Etheridge, Tom; Oland, C. Barry

2013-01-01

The Oak Ridge National Laboratory (ORNL) is a US Department of Energy (DOE) facility that is managed by UT-Battelle, LLC. In February 2006, DOE promulgated worker safety and health regulations to govern contractor activities at DOE sites. These regulations, which are provided in 10 CFR 851, Worker Safety and Health Program, establish requirements for worker safety and health program that reduce or prevent occupational injuries, illnesses, and accidental losses by providing DOE contractors and their workers with safe and healthful workplaces at DOE sites. The regulations state that contractors must achieve compliance no later than May 25, 2007. According tomore » 10 CFR 851, Subpart C, Specific Program Requirements, contractors must have a structured approach to their worker safety and health programs that at a minimum includes provisions for pressure safety. In implementing the structured approach for pressure safety, contractors must establish safety policies and procedures to ensure that pressure systems are designed, fabricated, tested, inspected, maintained, repaired, and operated by trained, qualified personnel in accordance with applicable sound engineering principles. In addition, contractors must ensure that all pressure vessels, boilers, air receivers, and supporting piping systems conform to (1) applicable American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code (2004) Sections I through XII, including applicable code cases; (2) applicable ASME B31 piping codes; and (3) the strictest applicable state and local codes. When national consensus codes are not applicable because of pressure range, vessel geometry, use of special materials, etc., contractors must implement measures to provide equivalent protection and ensure a level of safety greater than or equal to the level of protection afforded by the ASME or applicable state or local codes. This report documents the work performed to address legacy pressure vessel deficiencies and comply with pressure safety requirements in 10 CFR 851. It also describes actions taken to develop and implement ORNL’s Pressure Safety Program.« less

76 FR 62762 - Certain Large Diameter Carbon and Alloy Seamless Standard, Line and Pressure Pipe From Japan...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-11

... line applications such as oil, gas, or water pipeline, or utility distribution systems. Seamless pressure pipes are intended for the conveyance of water, steam, petrochemicals, chemicals, oil products... Fahrenheit, at various American Society of Mechanical Engineers (``ASME'') code stress levels. Alloy pipes...

The First ASME Code Stamped Cryomodule at SNS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Howell, M P; Crofford, M T; Douglas, D L

The first spare cryomodule for the Spallation Neutron Source (SNS) has been designed, fabricated, and tested by SNS personnel. The approach to design for this cryomodule was to hold critical design features identical to the original design such as bayonet positions, coupler positions, cold mass assembly, and overall footprint. However, this is the first SNS cryomodule that meets the pressure requirements put forth in the 10 CFR 851: Worker Safety and Health Program. The most significant difference is that Section VIII of the ASME Boiler and Pressure Vessel Code was applied to the vacuum vessel of this cryomodule. Applying themore » pressure code to the helium vessels within the cryomodule was considered. However, it was determined to be schedule prohibitive because it required a code case for materials that are not currently covered by the code. Good engineering practice was applied to the internal components to verify the quality and integrity of the entire cryomodule. The design of the cryomodule, fabrication effort, and cryogenic test results will be reported in this paper.« less

Frame Synchronization Without Attached Sync Markers

NASA Technical Reports Server (NTRS)

Hamkins, Jon

2011-01-01

We describe a method to synchronize codeword frames without making use of attached synchronization markers (ASMs). Instead, the synchronizer identifies the code structure present in the received symbols, by operating the decoder for a handful of iterations at each possible symbol offset and forming an appropriate metric. This method is computationally more complex and doesn't perform as well as frame synchronizers that utilize an ASM; nevertheless, the new synchronizer acquires frame synchronization in about two seconds when using a 600 kbps software decoder, and would take about 15 milliseconds on prototype hardware. It also eliminates the need for the ASMs, which is an attractive feature for short uplink codes whose coding gain would be diminished by the overheard of ASM bits. The lack of ASMs also would simplify clock distribution for the AR4JA low-density parity-check (LDPC) codes and adds a small amount to the coding gain as well (up to 0.2 dB).

46 CFR 57.02-2 - Adoption of section IX of the ASME Code.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 57.02-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING... qualifications for all types of welders and brazers, the qualification of welding procedures, and the production tests for all types of manual and machine arc and gas welding and brazing processes shall be in...

75 FR 1655 - Biweekly Notice Applications and Amendments to Facility Operating Licenses Involving No...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-12

... environmental impact statement or environmental assessment need be prepared for these amendments. If the... (ADAMS) Public Electronic Reading Room on the internet at the NRC Web site, http://www.nrc.gov/reading-rm... Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code, Section XI as the source of...

46 CFR 57.02-2 - Adoption of section IX of the ASME Code.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 57.02-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING... qualifications for all types of welders and brazers, the qualification of welding procedures, and the production tests for all types of manual and machine arc and gas welding and brazing processes shall be in...

46 CFR 57.02-2 - Adoption of section IX of the ASME Code.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 57.02-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING... qualifications for all types of welders and brazers, the qualification of welding procedures, and the production tests for all types of manual and machine arc and gas welding and brazing processes shall be in...

46 CFR 57.02-2 - Adoption of section IX of the ASME Code.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 57.02-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING... qualifications for all types of welders and brazers, the qualification of welding procedures, and the production tests for all types of manual and machine arc and gas welding and brazing processes shall be in...

46 CFR 57.02-2 - Adoption of section IX of the ASME Code.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 57.02-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING WELDING... qualifications for all types of welders and brazers, the qualification of welding procedures, and the production tests for all types of manual and machine arc and gas welding and brazing processes shall be in...

PHASE I MATERIALS PROPERTY DATABASE DEVELOPMENT FOR ASME CODES AND STANDARDS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ren, Weiju; Lin, Lianshan

2013-01-01

To support the ASME Boiler and Pressure Vessel Codes and Standard (BPVC) in modern information era, development of a web-based materials property database is initiated under the supervision of ASME Committee on Materials. To achieve efficiency, the project heavily draws upon experience from development of the Gen IV Materials Handbook and the Nuclear System Materials Handbook. The effort is divided into two phases. Phase I is planned to deliver a materials data file warehouse that offers a depository for various files containing raw data and background information, and Phase II will provide a relational digital database that provides advanced featuresmore » facilitating digital data processing and management. Population of the database will start with materials property data for nuclear applications and expand to data covering the entire ASME Code and Standards including the piping codes as the database structure is continuously optimized. The ultimate goal of the effort is to establish a sound cyber infrastructure that support ASME Codes and Standards development and maintenance.« less

Implementation of algebraic stress models in a general 3-D Navier-Stokes method (PAB3D)

NASA Technical Reports Server (NTRS)

Abdol-Hamid, Khaled S.

1995-01-01

A three-dimensional multiblock Navier-Stokes code, PAB3D, which was developed for propulsion integration and general aerodynamic analysis, has been used extensively by NASA Langley and other organizations to perform both internal (exhaust) and external flow analysis of complex aircraft configurations. This code was designed to solve the simplified Reynolds Averaged Navier-Stokes equations. A two-equation k-epsilon turbulence model has been used with considerable success, especially for attached flows. Accurate predicting of transonic shock wave location and pressure recovery in separated flow regions has been more difficult. Two algebraic Reynolds stress models (ASM) have been recently implemented in the code that greatly improved the code's ability to predict these difficult flow conditions. Good agreement with Direct Numerical Simulation (DNS) for a subsonic flat plate was achieved with ASM's developed by Shih, Zhu, and Lumley and Gatski and Speziale. Good predictions were also achieved at subsonic and transonic Mach numbers for shock location and trailing edge boattail pressure recovery on a single-engine afterbody/nozzle model.

76 FR 47555 - Certain Large Diameter Carbon and Alloy Seamless Standard, Line and Pressure Pipe From Japan...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-05

... diameter seamless pipe is used primarily for line applications such as oil, gas, or water pipeline, or utility distribution systems. Seamless pressure pipes are intended for the conveyance of water, steam... Engineers (``ASME'') code stress levels. Alloy pipes made to ASTM A-335 standard must be used if...

ITER's Tokamak Cooling Water System and the the Use of ASME Codes to Comply with French Regulations of Nuclear Pressure Equipment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berry, Jan; Ferrada, Juan J; Curd, Warren

During inductive plasma operation of ITER, fusion power will reach 500 MW with an energy multiplication factor of 10. The heat will be transferred by the Tokamak Cooling Water System (TCWS) to the environment using the secondary cooling system. Plasma operations are inherently safe even under the most severe postulated accident condition a large, in-vessel break that results in a loss-of-coolant accident. A functioning cooling water system is not required to ensure safe shutdown. Even though ITER is inherently safe, TCWS equipment (e.g., heat exchangers, piping, pressurizers) are classified as safety important components. This is because the water is predictedmore » to contain low-levels of radionuclides (e.g., activated corrosion products, tritium) with activity levels high enough to require the design of components to be in accordance with French regulations for nuclear pressure equipment, i.e., the French Order dated 12 December 2005 (ESPN). ESPN has extended the practical application of the methodology established by the Pressure Equipment Directive (97/23/EC) to nuclear pressure equipment, under French Decree 99-1046 dated 13 December 1999, and Order dated 21 December 1999 (ESP). ASME codes and supplementary analyses (e.g., Failure Modes and Effects Analysis) will be used to demonstrate that the TCWS equipment meets these essential safety requirements. TCWS is being designed to provide not only cooling, with a capacity of approximately 1 GW energy removal, but also elevated temperature baking of first-wall/blanket, vacuum vessel, and divertor. Additional TCWS functions include chemical control of water, draining and drying for maintenance, and facilitation of leak detection/localization. The TCWS interfaces with the majority of ITER systems, including the secondary cooling system. U.S. ITER is responsible for design, engineering, and procurement of the TCWS with industry support from an Engineering Services Organization (ESO) (AREVA Federal Services, with support from Northrop Grumman, and OneCIS). ITER International Organization (ITER-IO) is responsible for design oversight and equipment installation in Cadarache, France. TCWS equipment will be fabricated using ASME design codes with quality assurance and oversight by an Agreed Notified Body (approved by the French regulator) that will ensure regulatory compliance. This paper describes the TCWS design and how U.S. ITER and fabricators will use ASME codes to comply with EU Directives and French Orders and Decrees.« less

Turbulent Transport at High Reynolds Numbers in an Inertial Confinement Fusion Context

DTIC Science & Technology

2014-09-01

Department of Applied Mathematics and Statistics, Stony Brook University, Stony Brook, NY 11794 P . Rao1 Department of Applied Mathematics and Statistics...scales, 1Corresponding author. Contributed by the Fluids Engineering Division of ASME for publication in the JOURNAL OF FLUIDS ENGINEERING...Engineering SEPTEMBER 2014, Vol. 136 / 091206-1Copyright VC 2014 by ASME Downloaded From: http://fluidsengineering.asmedigitalcollection.asme.org/ on

Ultrasonic Inspection and Fatigue Evaluation of Critical Pore Size in Welds.

DTIC Science & Technology

1981-09-01

Boiler and Pressure Vessel Code ) 20...Five porosity levels were produced that parallelled ASME boiler and pressure vessel code specification (Section VIII). Appendix IV of the pressure...Figure 2 shows porosity charts (ASME Boiler and Pressure Vessel Code ) which classify and designate the number and size of pores in any six inch length

Status of Metric Conversion A Survey of U.S. Standards Writing Organizations.

DTIC Science & Technology

1982-05-01

Boiler and Pressure Vessel Code . 7...to and consistent with metrication of the ASME Boiler and Pressure Vessel Code . The Electrical Apparatus Service Association is a trade asso- ciation...metrication of TEMA Standards will be compatible to and consistent with metrication of the ASME Boiler and Pressure Vessel Code . TEMA’s metrication

Supercritical and Transcritical Shear Flows in Microgravity: Experiments and Direct Numerical Simulations

DTIC Science & Technology

2006-08-01

Boiler and Pressure Vessel Code were con...GRC, and to specifically state a general operating requirement. 1.1. The entire apparatus will be designed to ASME Boiler and Pressure Vessel Code , whenever...calculations, including a finite element analysis (FEA) will be inspected to verify the ASME Boiler and Pressure Vessel Code has been me, whenever

Review and Application of ASME NOG-1 and ASME NUM-1-2000

NASA Technical Reports Server (NTRS)

Lytle, Bradford P.; Delgado, H. (Technical Monitor)

2002-01-01

The intent of the workshop is to review the application of the ASME Nuclear Crane Standards ASME NOG-1 and ASME NUM-1-2000. The ASME Nuclear Crane standards provide a basis for purchasing overhead handling equipment with enhanced safety features, based upon accepted engineering principles, and including performance and environmental parameters specific to nuclear facilities.

75 FR 61530 - Issuance of Regulatory Guides

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-05

... Materials Code Case Acceptability, ASME Section III,'' and RG 1.147, Rev. 16, ``Inservice Inspection Code Case Acceptability, ASME Section XI, Division 1.'' FOR FURTHER INFORMATION CONTACT: Wallace E. Norris... specific problems or postulated accidents, and data the staff needs in its review of applications for...

DOD Residential Proton Exchange Membrane (PEM) Fuel Cell Demonstration Program. Volume 1. Summary of the Fiscal Year 2001 Program

DTIC Science & Technology

2004-02-01

Potential new stan- dard ASME Boiler and Pressure Vessel Code, Section VIII ( BPVC -VIII), Division 1 Rules for Construction of Pressure Vessels...Published and avail- able for sale. ASME BPVC -VIII Division 2 Rules for Construction of Pressure Vessels, Division 2, Gerry Eisenberg, ASME ...Vessels, Division 3, Alternate ASME BPVC -VIII Division 3 Gerry Eisenberg, ASME Published and avail- able for sale. Rules High

USAF Hyperbaric Animal Transfer Chamber System.

DTIC Science & Technology

1988-01-01

in full accordance with the requirements of the ASME Boiler and Pressure Vessel Code , Section VIII, Division 2, including provisions for lethal and...possible application to military and aviation medicine. REFERENCES 1. ASME Boiler and Pressure Vessel Code , Sec III, Div 2, para AD-160, AF-402, . and

Alloy-steel nuts for bolting for high-pressure and high-temperature service (ASME SA-194 with additional requirements)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This standard covers alloy steel nuts for bolting for high-pressure and high-temperature service in nuclear and associated applications. This standard does not cover bar or other starting materials. The only implied special considerations for starting materials are that they be capable of passing the required tests when processed into finished products in accordance with this standard. Material shall conform to the requirements of ASME SA-194; to the requirements of the ASME Boiler and Pressure Vessel Code (ASME Code), Section III, Article NB-2000; to the requirements of NE E 8-18; and to the additional requirements of this standard.

Structural Integrity of Water Reactor Pressure Boundary Components.

DTIC Science & Technology

1980-08-01

Boiler and Pressure Vessel Code , Sec. Ill). Estimates of the upper shelf K level from small-specimen...from Appendix A of Section XI of the ASME Boiler and Pressure Vessel Code [11. Figure 9 shows this same data set, together with earlier data for...0969, NRL Memo- randum Report 4063, Sep. 1979. 11. Section XI - ASME Boiler and Pressure Vessel Code , Rules for Inservice Inspection of Nuclear

Improving Shipbuilding Productivity Through Use of Standards

DTIC Science & Technology

1978-06-01

ship- building industry. In addition to the more familiar standards (e.g. ASME Boiler and Pressure Vessel Code , IEEE-45, etc.) this will include an...will simply refer- ence valid standards as appropriate (e.g. ASME Boiler and Pressure Vessel Code ), and will hopefully work hand in hand with the

49 CFR 178.337-3 - Structural integrity.

Code of Federal Regulations, 2011 CFR

2011-10-01

... stress at any point in the cargo tank may not exceed the maximum allowable stress value prescribed in... ASME Code or the ASTM standard to which the material is manufactured. (3) The maximum design stress at... ASME Code. The cargo tank design must include calculation of stresses generated by design pressure, the...

Implementation of ASME Code, Section XI, Code Case N-770, on Alternative Examination Requirements for Class 1 Butt Welds Fabricated with Alloy 82/182

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sullivan, Edmund J.; Anderson, Michael T.

In May 2010, the NRC issued a proposed notice of rulemaking that includes a provision to add a new section to its rules to require licensees to implement ASME Code Case N-770, ‘‘Alternative Examination Requirements and Acceptance Standards for Class 1 PWR Piping and Vessel Nozzle Butt Welds Fabricated with UNS N06082 or UNS W86182 Weld Filler Material With or Without the Application of Listed Mitigation Activities, Section XI, Division 1,’’ with 15 conditions. Code Case N-770 contains baseline and inservice inspection (ISI) requirements for unmitigated butt welds fabricated with Alloy 82/182 material and preservice and ISI requirements for mitigatedmore » butt welds. The NRC stated that application of ASME Code Case N-770 is necessary because the inspections currently required by the ASME Code, Section XI, were not written to address stress corrosion cracking Alloy 82/182 butt welds, and the safety consequences of inadequate inspections can be significant. The NRC expects to issue the final rule incorporating this code case into its regulations in the spring 2011 time frame. This paper discusses the new examination requirements, the conditions that NRC is imposing , and the major concerns with implementation of the new Code Case.« less

Ocean Engineering Studies Compiled 1991. Volume 6. Acrylic Windows - Typical Applications in Pressure Housings

DTIC Science & Technology

1991-01-01

either the metallic or plastic composite pressure envelope. The ASME Boiler and Pressure Vessel Code Section 8 provides such design criteria, and the...fabricated of metallic or piastic composite materials. To preclude potential catastrophic failures of windows designed on the basis of inadequate data, in...pressure-resistant acrylic windows (reference 12). Acrylic windows are usually machined from Plexiglas G plate, which is limited in thickness to 4 inches

The multiaxial fatigue response of cylindrical geometry under proportional loading subject to fluctuating tractions

NASA Astrophysics Data System (ADS)

Martinez, Rudy D.

A multiaxial fatigue model is proposed, as it would apply to cylindrical geometry in the form of industrial sized pressure vessels. The main focus of the multiaxial fatigue model will be based on using energy methods with the loading states confined to fluctuating tractions under proportional loading. The proposed fatigue model is an effort to support and enhance existing fatigue life predicting methods for pressure vessel design, beyond the ASME Boiler and Pressure Vessel codes, ASME Section VIII Division 2 and 3, which is currently used in industrial engineering practice for pressure vessel design. Both uniaxial and biaxial low alloy pearlittic-ferritic steel cylindrical cyclic test data are utilized to substantiate the proposed fatigue model. Approximate material hardening and softening aspects from applied load cycling states and the Bauschinger effect are accounted for by adjusting strain control generated hysteresis loops and the cyclic stress strain curve. The proposed fatigue energy model and the current ASME fatigue model are then compared with regards to the accuracy of predicting fatigue life cycle consistencies.

A beamline systems model for Accelerator-Driven Transmutation Technology (ADTT) facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Todd, A.M.M.; Paulson, C.C.; Peacock, M.A.

1995-10-01

A beamline systems code, that is being developed for Accelerator-Driven Transmutation Technology (ADTT) facility trade studies, is described. The overall program is a joint Grumman, G.H. Gillespie Associates (GHGA) and Los Alamos National Laboratory effort. The GHGA Accelerator Systems Model (ASM) has been adopted as the framework on which this effort is based. Relevant accelerator and beam transport models from earlier Grumman systems codes are being adapted to this framework. Preliminary physics and engineering models for each ADTT beamline component have been constructed. Examples noted include a Bridge Coupled Drift Tube Linac (BCDTL) and the accelerator thermal system. A decisionmore » has been made to confine the ASM framework principally to beamline modeling, while detailed target/blanket, balance-of-plant and facility costing analysis will be performed externally. An interfacing external balance-of-plant and facility costing model, which will permit the performance of iterative facility trade studies, is under separate development. An ABC (Accelerator Based Conversion) example is used to highlight the present models and capabilities.« less

A beamline systems model for Accelerator-Driven Transmutation Technology (ADTT) facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Todd, Alan M. M.; Paulson, C. C.; Peacock, M. A.

1995-09-15

A beamline systems code, that is being developed for Accelerator-Driven Transmutation Technology (ADTT) facility trade studies, is described. The overall program is a joint Grumman, G. H. Gillespie Associates (GHGA) and Los Alamos National Laboratory effort. The GHGA Accelerator Systems Model (ASM) has been adopted as the framework on which this effort is based. Relevant accelerator and beam transport models from earlier Grumman systems codes are being adapted to this framework. Preliminary physics and engineering models for each ADTT beamline component have been constructed. Examples noted include a Bridge Coupled Drift Tube Linac (BCDTL) and the accelerator thermal system. Amore » decision has been made to confine the ASM framework principally to beamline modeling, while detailed target/blanket, balance-of-plant and facility costing analysis will be performed externally. An interfacing external balance-of-plant and facility costing model, which will permit the performance of iterative facility trade studies, is under separate development. An ABC (Accelerator Based Conversion) example is used to highlight the present models and capabilities.« less

46 CFR 52.01-135 - Inspection and tests (modifies PG-90 through PG-100).

Code of Federal Regulations, 2010 CFR

2010-10-01

...). (a) Requirements. Inspection and test of boilers and boiler pressure parts shall be as indicated in PG-90 through PG-100 of section I of the ASME Boiler and Pressure Vessel Code (incorporated by...-91 of section I of the ASME Boiler and Pressure Vessel Code (incorporated by reference; see 46 CFR 52...

Feasibility Study of Coal Gasification/Fuel Cell/Cogeneration Project, Fort Greely, Alaska Site. Preliminary Survey,

DTIC Science & Technology

1985-04-02

sothat oilconsumptior ASME Boiler and Pressure Vessel Code . can be measured. Hot water boiler plants with out- U1I Shell-and-tube type exchangers are...slopes possible to VIII of the ASME Boiler and Pressure Vessel Code . prevent rain or melting snow from penetrating into (2? Water will flow through the

49 CFR Appendix C to Part 222 - Guide to Establishing Quiet Zones

Code of Federal Regulations, 2011 CFR

2011-10-01

... Horns will not be subject to annual reviews. (5) The use of FRA's web-based Quiet Zone Calculator is... appendix A (e.g., shorter than required traffic channelization devices), non-engineering ASMs (e.g., programmed law enforcement), and engineering ASMs (i.e., engineering improvements other than modified SSMs...

Alternative splicing of SMPD1 coding for acid sphingomyelinase in major depression.

PubMed

Rhein, Cosima; Reichel, Martin; Kramer, Marcel; Rotter, Andrea; Lenz, Bernd; Mühle, Christiane; Gulbins, Erich; Kornhuber, Johannes

2017-02-01

Major depressive disorder (MDD) is a psychiatric disorder characterized by key symptoms that include depressed mood and a loss of interest and pleasure. A recently developed pathogenic model of MDD involves disturbed neurogenesis in the hippocampus, where the acid sphingomyelinase (ASM)/ceramide system plays an important role and is proposed as a molecular target for antidepressant action. Because alternative splicing of SMPD1 mRNA, coding for ASM, is relevant for the regulation of ASM enzymatic activity, we investigated the frequency of alternatively spliced ASM isoforms in peripheral blood cells of MDD patients versus healthy controls. Because the full-length transcript variant 1 of SMPD1 (termed ASM-1) is the only known form within the splicing pattern that encodes an enzymatically fully active ASM, we determined a fraction of splice isoforms deviating from ASM-1 using PCR amplification and capillary electrophoresis with laser-induced fluorescence analysis. ASM alternative splicing events occurred significantly less frequently in MDD patients compared to healthy subjects. After 5 days of antidepressant treatment, the frequency of alternatively spliced ASM isoforms decreased in those patients who were treated with a functional inhibitor of ASM activity (FIASMA) but remained constant in MDD patients treated with other antidepressant drugs. This effect was more pronounced when healthy male volunteers were treated with the FIASMAs fluoxetine or paroxetine, in contrast to a placebo group. Patients were treated with different antidepressant drugs, depending on individual parameters and disease courses. This study shows that the ASM alternative splicing pattern could be a biological target with diagnostic relevance and could serve as a novel biomarker for MDD. Copyright © 2016 Elsevier B.V. All rights reserved.

Report on FY15 alloy 617 code rules development

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sham, Sam; Jetter, Robert I; Hollinger, Greg

2015-09-01

Due to its strength at very high temperatures, up to 950°C (1742°F), Alloy 617 is the reference construction material for structural components that operate at or near the outlet temperature of the very high temperature gas-cooled reactors. However, the current rules in the ASME Section III, Division 5 Subsection HB, Subpart B for the evaluation of strain limits and creep-fatigue damage using simplified methods based on elastic analysis have been deemed inappropriate for Alloy 617 at temperatures above 650°C (1200°F) (Corum and Brass, Proceedings of ASME 1991 Pressure Vessels and Piping Conference, PVP-Vol. 215, p.147, ASME, NY, 1991). The rationalemore » for this exclusion is that at higher temperatures it is not feasible to decouple plasticity and creep, which is the basis for the current simplified rules. This temperature, 650°C (1200°F), is well below the temperature range of interest for this material for the high temperature gas-cooled reactors and the very high temperature gas-cooled reactors. The only current alternative is, thus, a full inelastic analysis requiring sophisticated material models that have not yet been formulated and verified. To address these issues, proposed code rules have been developed which are based on the use of elastic-perfectly plastic (EPP) analysis methods applicable to very high temperatures. The proposed rules for strain limits and creep-fatigue evaluation were initially documented in the technical literature (Carter, Jetter and Sham, Proceedings of ASME 2012 Pressure Vessels and Piping Conference, papers PVP 2012 28082 and PVP 2012 28083, ASME, NY, 2012), and have been recently revised to incorporate comments and simplify their application. Background documents have been developed for these two code cases to support the ASME Code committee approval process. These background documents for the EPP strain limits and creep-fatigue code cases are documented in this report.« less

High Temperature Gas Reactors: Assessment of Applicable Codes and Standards

DOE Office of Scientific and Technical Information (OSTI.GOV)

McDowell, Bruce K.; Nickolaus, James R.; Mitchell, Mark R.

2011-10-31

Current interest expressed by industry in HTGR plants, particularly modular plants with power up to about 600 MW(e) per unit, has prompted NRC to task PNNL with assessing the currently available literature related to codes and standards applicable to HTGR plants, the operating history of past and present HTGR plants, and with evaluating the proposed designs of RPV and associated piping for future plants. Considering these topics in the order they are arranged in the text, first the operational histories of five shut-down and two currently operating HTGR plants are reviewed, leading the authors to conclude that while small, simplemore » prototype HTGR plants operated reliably, some of the larger plants, particularly Fort St. Vrain, had poor availability. Safety and radiological performance of these plants has been considerably better than LWR plants. Petroleum processing plants provide some applicable experience with materials similar to those proposed for HTGR piping and vessels. At least one currently operating plant - HTR-10 - has performed and documented a leak before break analysis that appears to be applicable to proposed future US HTGR designs. Current codes and standards cover some HTGR materials, but not all materials are covered to the high temperatures envisioned for HTGR use. Codes and standards, particularly ASME Codes, are under development for proposed future US HTGR designs. A 'roadmap' document has been prepared for ASME Code development; a new subsection to section III of the ASME Code, ASME BPVC III-5, is scheduled to be published in October 2011. The question of terminology for the cross-duct structure between the RPV and power conversion vessel is discussed, considering the differences in regulatory requirements that apply depending on whether this structure is designated as a 'vessel' or as a 'pipe'. We conclude that designing this component as a 'pipe' is the more appropriate choice, but that the ASME BPVC allows the owner of the facility to select the preferred designation, and that either designation can be acceptable.« less

46 CFR 64.2 - Incorporation by reference.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Incorporation by reference. 64.2 Section 64.2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING MARINE PORTABLE TANKS AND... Mechanical Engineers (ASME) International Three Park Avenue, New York, NY 10016-5990. ASME Boiler and...

46 CFR 64.2 - Incorporation by reference.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Incorporation by reference. 64.2 Section 64.2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING MARINE PORTABLE TANKS AND... Mechanical Engineers (ASME) International Three Park Avenue, New York, NY 10016-5990. ASME Boiler and...

46 CFR 56.60-1 - Acceptable materials and specifications (replaces 123 and Table 126.1 in ASME B31.1).

Code of Federal Regulations, 2010 CFR

2010-10-01

... Low temperature steel pipe Sec. VIII of the ASME Boiler and Pressure Vessel Code (5). Pipe, welded: A... only, fusion welded steel pipe ASME B31.1 (8). A 358 Electric fusion welded pipe, high temperature... Seamless and welded (no added filler metal) carbon and low alloy tubing for low temperature UCS23, Sec...

A Proposal for the Maximum KIC for Use in ASME Code Flaw and Fracture Toughness Evaluations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kirk, Mark; Stevens, Gary; Erickson, Marjorie A

2011-01-01

Nonmandatory Appendices A [1] and G [2] of Section XI of the ASME Code use the KIc curve (indexed to the material reference transition temperature, RTNDT) in reactor pressure vessel (RPV) flaw evaluations, and for the purpose of establishing RPV pressure-temperature (P-T) limits. Neither of these appendices places an upper-limit on the KIc value that may be used in these assessments. Over the years, it has often been suggested by some of the members of the ASME Section XI Code committees that are responsible for maintaining Appendices A and G that there is a practical upper limit of 200 ksimore » in (220 MPa m) [4]. This upper limit is not well recognized by all users of the ASME Code, is not explicitly documented within the Code itself, and the one source known to the authors where it is defended [4] relies on data that is either in error, or is less than 220 MPa m. However, as part of the NRC/industry pressurized thermal shock (PTS) re-evaluation effort, empirical models were developed that propose common temperature dependencies for all ferritic steels operating on the upper shelf. These models relate the fracture toughness properties in the transition regime to those on the upper shelf and, combined with data for a wide variety of RPV steels and welds on which they are based, suggest that the practical upper limit of 220 MPa m exceeds the upper shelf fracture toughness of most RPV steels by a considerable amount, especially for irradiated steels. In this paper, available models and data are used to propose upper bound limits of applicability on the KIc curve for use in ASME Code, Section XI, Nonmandatory Appendices A and G evaluations that are consistent with available data for RPV steels.« less

ASME Nuclear Crane Standards for Enhanced Crane Safety and Increased Profit

NASA Astrophysics Data System (ADS)

Parkhurst, Stephen N.

2000-01-01

The ASME NOG-1 standard, 'Rules for Construction of Overhead and Gantry Cranes', covers top running cranes for nuclear facilities; with the ASME NUM-1 standard, 'Rules for Construction of Cranes, Monorails, and Hoists', covering the single girder, underhung, wall and jib cranes, as well as the monorails and hoists. These two ASME nuclear crane standards provide criteria for designing, inspecting and testing overhead handling equipment with enhanced safety to meet the 'defense-in-depth' approach of the United States Nuclear Regulatory Commission (USNRC) documents NUREG 0554 and NUREG 0612. In addition to providing designs for enhanced safety, the ASME nuclear crane standards provide a basis for purchasing overhead handling equipment with standard safety features, based upon accepted engineering principles, and including performance and environmental parameters specific to nuclear facilities. The ASME NOG-1 and ASME NUM-1 standards not only provide enhanced safety for handling a critical load, but also increase profit by minimizing the possibility of load drops, by reducing cumbersome operating restrictions, and by providing the foundation for a sound licensing position. The ASME nuclear crane standards can also increase profit by providing the designs and information to help ensure that the right standard equipment is purchased. Additionally, the ASME nuclear crane standards can increase profit by providing designs and information to help address current issues, such as the qualification of nuclear plant cranes for making 'planned engineered lifts' for steam generator replacement and decommissioning.

TOWARD THE DEVELOPMENT OF A CONSENSUS MATERIALS DATABASE FOR PRESSURE TECHNOLGY APPLICATIONS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Swindeman, Robert W; Ren, Weiju

The ASME construction code books specify materials and fabrication procedures that are acceptable for pressure technology applications. However, with few exceptions, the materials properties provided in the ASME code books provide no statistics or other information pertaining to material variability. Such information is central to the prediction and prevention of failure events. Many sources of materials data exist that provide variability information but such sources do not necessarily represent a consensus of experts with respect to the reported trends that are represented. Such a need has been identified by the ASME Standards Technology, LLC and initial steps have been takenmore » to address these needs: however, these steps are limited to project-specific applications only, such as the joint DOE-ASME project on materials for Generation IV nuclear reactors. In contrast to light-water reactor technology, the experience base for the Generation IV nuclear reactors is somewhat lacking and heavy reliance must be placed on model development and predictive capability. The database for model development is being assembled and includes existing code alloys such as alloy 800H and 9Cr-1Mo-V steel. Ownership and use rights are potential barriers that must be addressed.« less

EVALUATION OF ULTRASONIC PHASED-ARRAY FOR DETECTION OF PLANAR FLAWS IN HIGH-DENSITY POLYETHYLENE (HDPE) BUTT-FUSION JOINTS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prowant, Matthew S.; Denslow, Kayte M.; Moran, Traci L.

2016-09-21

The desire to use high-density polyethylene (HDPE) piping in buried Class 3 service and cooling water systems in nuclear power plants is primarily motivated by the material’s high resistance to corrosion relative to that of steel and metal alloys. The rules for construction of Class 3 HDPE pressure piping systems were originally published in Code Case N-755 and were recently incorporated into the American Society of Mechanical Engineers Boiler and Pressure Vessel Code (ASME BPVC) Section III as Mandatory Appendix XXVI (2015 Edition). The requirements for HDPE examination are guided by criteria developed for metal pipe and are based onmore » industry-led HDPE research or conservative calculations.« less

GTA Welding Research and Development for Plutonium Containment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sessions, C.E.

2002-02-21

This paper discusses the development of two welding systems that are used to contain actinide metals and oxides for long term storage. The systems are termed the bagless transfer system (BTS) and the outer container welder (OCW) system. The BTS is so named because it permits the containment of actinides without a polymeric package (i.e., bag). The development of these two systems was directed by Department of Energy Standard 3013, hereafter referred to as DOE 3013. This document defines the product and container requirements. In addition, it references national codes and standards for leak rates, ANSI N14.5, and design, Americanmore » Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code Section VIII (BandPVC).« less

Deterministic Local Sensitivity Analysis of Augmented Systems - II: Applications to the QUENCH-04 Experiment Using the RELAP5/MOD3.2 Code System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ionescu-Bujor, Mihaela; Jin Xuezhou; Cacuci, Dan G.

2005-09-15

The adjoint sensitivity analysis procedure for augmented systems for application to the RELAP5/MOD3.2 code system is illustrated. Specifically, the adjoint sensitivity model corresponding to the heat structure models in RELAP5/MOD3.2 is derived and subsequently augmented to the two-fluid adjoint sensitivity model (ASM-REL/TF). The end product, called ASM-REL/TFH, comprises the complete adjoint sensitivity model for the coupled fluid dynamics/heat structure packages of the large-scale simulation code RELAP5/MOD3.2. The ASM-REL/TFH model is validated by computing sensitivities to the initial conditions for various time-dependent temperatures in the test bundle of the Quench-04 reactor safety experiment. This experiment simulates the reflooding with water ofmore » uncovered, degraded fuel rods, clad with material (Zircaloy-4) that has the same composition and size as that used in typical pressurized water reactors. The most important response for the Quench-04 experiment is the time evolution of the cladding temperature of heated fuel rods. The ASM-REL/TFH model is subsequently used to perform an illustrative sensitivity analysis of this and other time-dependent temperatures within the bundle. The results computed by using the augmented adjoint sensitivity system, ASM-REL/TFH, highlight the reliability, efficiency, and usefulness of the adjoint sensitivity analysis procedure for computing time-dependent sensitivities.« less

Langley Research Center Standard for the Evaluation of Socket Welds

NASA Technical Reports Server (NTRS)

Berry, R. F., Jr.

1985-01-01

A specification utilized for the nondestructive evaluation of socket type pipe joints at Langley Research Center (LaRC) is discussed. The scope of hardware shall include, but is not limited to, all common pipe fittings: tees, elbows, couplings, caps, and so forth, socket type flanges, unions, and valves. In addition, the exterior weld of slip on flanges shall be inspected using this specification. At the discretion of the design engineer, standard practice engineer, Fracture Mechanics Engineering Section, Pressure Systems Committee, or other authority, four nondestructive evaluation techniques may be utilized exclusively, or in combination, to inspect socket type welds. These techniques are visual, radiographic, magnetic particle, and dye penetrant. Under special circumstances, other techniques (such as eddy current or ultrasonics) may be required and their application shall be guided by the appropriate sections of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code (B&PVC).

Creep-Rupture Behavior of Ni-Based Alloy Tube Bends for A-USC Boilers

NASA Astrophysics Data System (ADS)

Shingledecker, John

Advanced ultrasupercritical (A-USC) boiler designs will require the use of nickel-based alloys for superheaters and reheaters and thus tube bending will be required. The American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code Section II PG-19 limits the amount of cold-strain for boiler tube bends for austenitic materials. In this summary and analysis of research conducted to date, a number of candidate nickel-based A-USC alloys were evaluated. These alloys include alloy 230, alloy 617, and Inconel 740/740H. Uniaxial creep and novel structural tests and corresponding post-test analysis, which included physical measurements, simplified analytical analysis, and detailed microscopy, showed that different damage mechanisms may operate based on test conditions, alloy, and cold-strain levels. Overall, creep strength and ductility were reduced in all the alloys, but the degree of degradation varied substantially. The results support the current cold-strain limits now incorporated in ASME for these alloys for long-term A-USC boiler service.

REVIEW OF PROPOSED METHODOLOGY FOR A RISK- INFORMED RELAXATION TO ASME SECTION XI APPENDIX G

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dickson, Terry L; Kirk, Mark

2010-01-01

The current regulations, as set forth by the United States Nuclear Regulatory Commission (NRC), to insure that light-water nuclear reactor pressure vessels (RPVs) maintain their structural integrity when subjected to planned normal reactor startup (heat-up) and shut-down (cool-down) transients are specified in Appendix G to 10 CFR Part 50, which incorporates by reference Appendix G to Section XI of the American Society of Mechanical Engineers (ASME) Code. The technical basis for these regulations are now recognized by the technical community as being conservative and some plants are finding it increasingly difficult to comply with the current regulations. Consequently, the nuclearmore » industry has developed, and submitted to the ASME Code for approval, an alternative risk-informed methodology that reduces the conservatism and is consistent with the methods previously used to develop a risk-informed revision to the regulations for accidental transients such as pressurized thermal shock (PTS). The objective of the alternative methodology is to provide a relaxation to the current regulations which will provide more operational flexibility, particularly for reactor pressure vessels with relatively high irradiation levels and radiation sensitive materials, while continuing to provide reasonable assurance of adequate protection to public health and safety. The NRC and its contractor at Oak Ridge National Laboratory (ORNL) have recently performed an independent review of the industry proposed methodology. The NRC / ORNL review consisted of performing probabilistic fracture mechanics (PFM) analyses for a matrix of cool-down and heat-up rates, permutated over various reactor geometries and characteristics, each at multiple levels of embrittlement, including 60 effective full power years (EFPY) and beyond, for various postulated flaw characterizations. The objective of this review is to quantify the risk of a reactor vessel experiencing non-ductile fracture, and possible subsequent failure, over a wide range of normal transient conditions, when the maximum allowable thermal-hydraulic boundary conditions, derived from both the current ASME code and the industry proposed methodology, are imposed on the inner surface of the reactor vessel. This paper discusses the results of the NRC/ORNL review of the industry proposal including the matrices of PFM analyses, results, insights, and conclusions derived from these analyses.« less

Dynamic fracture toughness of ASME SA508 Class 2a ASME SA533 grade A Class 2 base and heat affected zone material and applicable weld metals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Logsdon, W.A.; Begley, J.A.; Gottshall, C.L.

1978-03-01

The ASME Boiler and Pressure Vessel Code, Section III, Article G-2000, requires that dynamic fracture toughness data be developed for materials with specified minimum yield strengths greater than 50 ksi to provide verification and utilization of the ASME specified minimum reference toughness K/sub IR/ curve. In order to qualify ASME SA508 Class 2a and ASME SA533 Grade A Class 2 pressure vessel steels (minimum yield strengths equal 65 kip/in./sup 2/ and 70 kip/in./sup 2/, respectively) per this requirement, dynamic fracture toughness tests were performed on these materials. All dynamic fracture toughness values of SA508 Class 2a base and HAZ material,more » SA533 Grade A Class 2 base and HAZ material, and applicable weld metals exceeded the ASME specified minimum reference toughness K/sub IR/ curve.« less

Performance, Life, and Operability Trade-Offs in VCE Control Logic Design.

DTIC Science & Technology

1981-08-01

primarily USAF Actuarial Reports), including all engine returns to the intermediate or depot shop - scheduled and unscheduled - engine and non-engine...Length (Min) Training * Air to Air (ATA) 30 576 87 e Air to Ground (ATG) 30 363 128 Combat e Air Superiority ( ASM ) 13 75 228 e Intercept 2 31 94... ASME , 16th Joint Propulsion Conference, Paper No. ALAA-80-1115, June 30 - July 2, 1980. 2. Akimov, V.M., Starik, D.E., et al., The Economic Efficiency

76 FR 11191 - Hazardous Materials: Adoption of ASME Code Section XII and the National Board Inspection Code

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-01

... parts of the National Board Inspection Code at http://www.nationalboard.org . DATES: The comment period... edition of the National Board Inspection Code for public review at www.nationalboard.org . Both documents...

Characterization and machine learning prediction of allele-specific DNA methylation.

PubMed

He, Jianlin; Sun, Ming-an; Wang, Zhong; Wang, Qianfei; Li, Qing; Xie, Hehuang

2015-12-01

A large collection of Single Nucleotide Polymorphisms (SNPs) has been identified in the human genome. Currently, the epigenetic influences of SNPs on their neighboring CpG sites remain elusive. A growing body of evidence suggests that locus-specific information, including genomic features and local epigenetic state, may play important roles in the epigenetic readout of SNPs. In this study, we made use of mouse methylomes with known SNPs to develop statistical models for the prediction of SNP associated allele-specific DNA methylation (ASM). ASM has been classified into parent-of-origin dependent ASM (P-ASM) and sequence-dependent ASM (S-ASM), which comprises scattered-S-ASM (sS-ASM) and clustered-S-ASM (cS-ASM). We found that P-ASM and cS-ASM CpG sites are both enriched in CpG rich regions, promoters and exons, while sS-ASM CpG sites are enriched in simple repeat and regions with high frequent SNP occurrence. Using Lasso-grouped Logistic Regression (LGLR), we selected 21 out of 282 genomic and methylation related features that are powerful in distinguishing cS-ASM CpG sites and trained the classifiers with machine learning techniques. Based on 5-fold cross-validation, the logistic regression classifier was found to be the best for cS-ASM prediction with an ACC of 0.77, an AUC of 0.84 and an MCC of 0.54. Lastly, we applied the logistic regression classifier on human brain methylome and predicted 608 genes associated with cS-ASM. Gene ontology term enrichment analysis indicated that these cS-ASM associated genes are significantly enriched in the category coding for transcripts with alternative splicing forms. In summary, this study provided an analytical procedure for cS-ASM prediction and shed new light on the understanding of different types of ASM events. Published by Elsevier Inc.

46 CFR 56.60-2 - Limitations on materials.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Boiler and Pressure Vessel Code * ASTM specifications Source of allowable stress Notes Ferrous Materials...-5. 2 Allowable stresses shall be the same as those listed in UCS23 of section VIII of the ASME.... 4 Allowable stresses shall be the same as those listed in UCS23 of section VIII of the ASME Boiler...

46 CFR 56.60-2 - Limitations on materials.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Boiler and Pressure Vessel Code * ASTM specifications Source of allowable stress Notes Ferrous Materials...-5. 2 Allowable stresses shall be the same as those listed in UCS23 of section VIII of the ASME.... 4 Allowable stresses shall be the same as those listed in UCS23 of section VIII of the ASME Boiler...

29 CFR 1926.6 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-07-01

...-mail: [email protected]; Web site: http://www.asme.org/: (1) ASME B30.2-2005, Overhead and Gantry....1501(a). (2) ANSI B30.2.0-1967, Safety Code for Overhead and Gantry Cranes, approved May 4, 1967, IBR..., Washington, DC 20210. (2) The Regional and Field Offices of the Occupational Safety and Health Administration...

History of the Fluids Engineering Division

DOE PAGES

Cooper, Paul; Martin, C. Samuel; O'Hern, Timothy J.

2016-08-03

The 90th Anniversary of the Fluids Engineering Division (FED) of ASME will be celebrated on July 10–14, 2016 in Washington, DC. The venue is ASME's Summer Heat Transfer Conference (SHTC), Fluids Engineering Division Summer Meeting (FEDSM), and International Conference on Nanochannels and Microchannels (ICNMM). The occasion is an opportune time to celebrate and reflect on the origin of FED and its predecessor—the Hydraulic Division (HYD), which existed from 1926–1963. Furthermore, the FED Executive Committee decided that it would be appropriate to publish concurrently a history of the HYD/FED.

History of the Fluids Engineering Division

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cooper, Paul; Martin, C. Samuel; O'Hern, Timothy J.

The 90th Anniversary of the Fluids Engineering Division (FED) of ASME will be celebrated on July 10–14, 2016 in Washington, DC. The venue is ASME's Summer Heat Transfer Conference (SHTC), Fluids Engineering Division Summer Meeting (FEDSM), and International Conference on Nanochannels and Microchannels (ICNMM). The occasion is an opportune time to celebrate and reflect on the origin of FED and its predecessor—the Hydraulic Division (HYD), which existed from 1926–1963. Furthermore, the FED Executive Committee decided that it would be appropriate to publish concurrently a history of the HYD/FED.

ASME AG-1 Section FC Qualified HEPA Filters; a Particle Loading Comparison - 13435

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stillo, Andrew; Ricketts, Craig I.

High Efficiency Particulate Air (HEPA) Filters used to protect personnel, the public and the environment from airborne radioactive materials are designed, manufactured and qualified in accordance with ASME AG-1 Code section FC (HEPA Filters) [1]. The qualification process requires that filters manufactured in accordance with this ASME AG-1 code section must meet several performance requirements. These requirements include performance specifications for resistance to airflow, aerosol penetration, resistance to rough handling, resistance to pressure (includes high humidity and water droplet exposure), resistance to heated air, spot flame resistance and a visual/dimensional inspection. None of these requirements evaluate the particle loading capacitymore » of a HEPA filter design. Concerns, over the particle loading capacity, of the different designs included within the ASME AG-1 section FC code[1], have been voiced in the recent past. Additionally, the ability of a filter to maintain its integrity, if subjected to severe operating conditions such as elevated relative humidity, fog conditions or elevated temperature, after loading in use over long service intervals is also a major concern. Although currently qualified HEPA filter media are likely to have similar loading characteristics when evaluated independently, filter pleat geometry can have a significant impact on the in-situ particle loading capacity of filter packs. Aerosol particle characteristics, such as size and composition, may also have a significant impact on filter loading capacity. Test results comparing filter loading capacities for three different aerosol particles and three different filter pack configurations are reviewed. The information presented represents an empirical performance comparison among the filter designs tested. The results may serve as a basis for further discussion toward the possible development of a particle loading test to be included in the qualification requirements of ASME AG-1 Code sections FC and FK[1]. (authors)« less

Effect of Light Water Reactor Water Environments on the Fatigue Life of Reactor Materials

DOE PAGES

Chopra, O. K.; Stevens, G. L.; Tregoning, R.; ...

2017-10-06

The American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code (Code) provides rules for the design of Class 1 components of nuclear power plants. Figures I-9.1 through I-9.6 of Appendix I to Section III of the Code specify fatigue design curves for applicable structural materials. However, the Code design curves do not explicitly address the effects of light water reactor (LWR) water environments. Existing fatigue strain-vs.-life (ε-N) laboratory data illustrate potentially significant effects of LWR water environments on the fatigue resistance of pressure vessel and piping steels. Extensive studies have been conducted at Argonne National Laboratory and elsewheremore » since 1990 to investigate the effects of LWR environments on the fatigue life of piping and pressure vessel steels. This article summarizes the results of these studies. Existing fatigue ε-N data were evaluated to identify the various material, environmental, and loading conditions that influence fatigue crack initiation; a methodology for estimating fatigue lives as a function of these parameters was developed. The effects were incorporated into the ASME Code Section III fatigue evaluations in terms of an environmental correction factor, F en, which is defined as the ratio of fatigue life in air at room temperature to the fatigue life in the LWR water environment at reactor operating temperatures. Available fatigue data were used to develop fatigue design curves for carbon and low-alloy steels, austenitic stainless steels, and nickel-chromium-iron (NiCr-Fe) alloys and their weld metals in air at room temperature. A review of the Code Section III fatigue adjustment factors of 2 on strain and 20 on life is also presented and the possible conservatism inherent in the choice of these adjustment factors is evaluated. A brief description of potential effects of neutron irradiation on fatigue crack initiation for these structural materials is also presented.« less

Effect of Light Water Reactor Water Environments on the Fatigue Life of Reactor Materials

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chopra, O. K.; Stevens, G. L.; Tregoning, R.

The American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code (Code) provides rules for the design of Class 1 components of nuclear power plants. Figures I-9.1 through I-9.6 of Appendix I to Section III of the Code specify fatigue design curves for applicable structural materials. However, the Code design curves do not explicitly address the effects of light water reactor (LWR) water environments. Existing fatigue strain-vs.-life (ε-N) laboratory data illustrate potentially significant effects of LWR water environments on the fatigue resistance of pressure vessel and piping steels. Extensive studies have been conducted at Argonne National Laboratory and elsewheremore » since 1990 to investigate the effects of LWR environments on the fatigue life of piping and pressure vessel steels. This article summarizes the results of these studies. Existing fatigue ε-N data were evaluated to identify the various material, environmental, and loading conditions that influence fatigue crack initiation; a methodology for estimating fatigue lives as a function of these parameters was developed. The effects were incorporated into the ASME Code Section III fatigue evaluations in terms of an environmental correction factor, F en, which is defined as the ratio of fatigue life in air at room temperature to the fatigue life in the LWR water environment at reactor operating temperatures. Available fatigue data were used to develop fatigue design curves for carbon and low-alloy steels, austenitic stainless steels, and nickel-chromium-iron (NiCr-Fe) alloys and their weld metals in air at room temperature. A review of the Code Section III fatigue adjustment factors of 2 on strain and 20 on life is also presented and the possible conservatism inherent in the choice of these adjustment factors is evaluated. A brief description of potential effects of neutron irradiation on fatigue crack initiation for these structural materials is also presented.« less

Hydrogen/Oxygen Propellant Densifier Thermoacoustic Stirling Heat Engine

NASA Astrophysics Data System (ADS)

Nguyen, C. T.; Yeckley, A. J.; Schieb, D. J.; Haberbusch, M. S.

2004-06-01

A unique, patent pending, thermoacoustic propellant densifier for the simultaneous densification of hydrogen and oxygen propellants for aerospace vehicles is introduced. The densifier uses a high-pressure amplitude, low-frequency Thermoacoustic Stirling Heat Engine (TASHE) coupled with a uniquely designed half-wave-length resonator to drive a pulse tube cryocooler using a Gas Helium (GHe) working fluid. The extremely reliable TASHE has no moving parts, is water cooled, and is electrically powered. The helium-filled TASHE is designed to ASME piping codes, which enables the safe inspection of the system while in operation. The resonator is designed to eliminate higher-order harmonics with minimal acoustic losses. A system description will be presented, and experimental data on both the TASHE and the resonator will be compared with analytical results.

49 CFR 230.8 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-10-01

....) NBIC. National Board Inspection Code published by the National Board of Boiler and Pressure Vessel.... American National Standards Institute. API. American Petroleum Institute. ASME. American Society of... separation into parts. Code of original construction. The manufacturer's or industry code in effect when the...

49 CFR 230.8 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-10-01

....) NBIC. National Board Inspection Code published by the National Board of Boiler and Pressure Vessel.... American National Standards Institute. API. American Petroleum Institute. ASME. American Society of... separation into parts. Code of original construction. The manufacturer's or industry code in effect when the...

49 CFR 230.8 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-10-01

....) NBIC. National Board Inspection Code published by the National Board of Boiler and Pressure Vessel.... American National Standards Institute. API. American Petroleum Institute. ASME. American Society of... separation into parts. Code of original construction. The manufacturer's or industry code in effect when the...

Lawriter - OAC

Science.gov Websites

authorized by section 3709.05 of the Revised Code. (C) "Circulation system" means an arrangement of transferred and does not mean the person who formerly operated or maintained the public swimming pool. (J ://catalog.asme.org/. ASME documents are also generally available at the state library of Ohio. (c) American national

FY17 Status Report on the Initial Development of a Constitutive Model for Grade 91 Steel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Messner, M. C.; Phan, V. -T.; Sham, T. -L.

Grade 91 is a candidate structural material for high temperature advanced reactor applications. Existing ASME Section III, Subsection HB, Subpart B simplified design rules based on elastic analysis are setup as conservative screening tools with the intent to supplement these screening rules with full inelastic analysis when required. The Code provides general guidelines for suitable inelastic models but does not provide constitutive model implementations. This report describes the development of an inelastic constitutive model for Gr. 91 steel aimed at fulfilling the ASME Code requirements and being included into a new Section III Code appendix, HBB-Z. A large database ofmore » over 300 experiments on Gr. 91 was collected and converted to a standard XML form. Five families of Gr. 91 material models were identified in the literature. Of these five, two are potentially suitable for use in the ASME code. These two models were implemented and evaluated against the experimental database. Both models have deficiencies so the report develops a framework for developing and calibrating an improved model. This required creating a new modeling method for representing changes in material rate sensitivity across the full ASME allowable temperature range for Gr. 91 structural components: room temperature to 650° C. On top of this framework for rate sensitivity the report describes calibrating a model for work hardening and softening in the material using genetic algorithm optimization. Future work will focus on improving this trial model by including tension/compression asymmetry observed in experiments and necessary to capture material ratcheting under zero mean stress and by improving the optimization and analysis framework.« less

46 CFR 56.01-2 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990... (“ASME SA-675”), 56.60-2. (e) ASTM International (formerly American Society for Testing and Materials... 15540 Ships and Marine Technology-Fire Resistance of Hose Assemblies-Test Methods, First Edition (Aug. 1...

Altitude Ignition/Lean Decel Study.

DTIC Science & Technology

1985-11-01

Pittsburgh 1977. 26. Moses C. A. and Naegeli D. W., "Fuel Property Effects on Combustor Performance," ASME 79-GT-178, Presented at the Gas Turbine...29. Naegeli , D. W., Moses, C. A. and Mellor, A. M., "Preliminary Correlation of Fuel Effects on Ignitability for Gas Turbine Engines," ASME Paper No

Biomass Economy

DTIC Science & Technology

1985-11-01

Boiler and Pressure Vessel Code HEI Heat Exchanger Institute Heat and Material Balance c. System Description (1) Condenser... Boiler and Pressure Vessel Code "AN(SI B31.1 Power Piping d. System Description (1) Deaerator The deaerator will be d direct contact feedwater heater, and...vent, and drain piping. "b . Applicable Codes ASME Boiler and Pressure Vessel Code "ANSI B31.1 - Power Piping Code

Interpreting ASME limits and philosophy in FEA of pressure vessel parts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bezerra, L.M.; Cruz, J.R.B.; Miranda, C.A.J.

1995-12-01

In recent years there has been an effort to interpret finite element (FE) stress results on the light of the ASME B and PV rules and philosophy. Many task groups have issued guidelines on stress linearization and classifications. All those attempts have come up trying to cope modern FE techniques with the rules imposed by the ASME Code. This paper is an independent contribution to the Pressure Vessel Research Council (PVRC) groups which are studying the stress classification and the failure mechanism in a FE framework. This work tries to complement the interesting work by Hollinger and Hechmer presented inmore » the PVP-94 in Minneapolis. In that paper, the authors examined a typical support skirt and showed relations between the skirt collapse load obtained by finite element analysis and the loads allowed from the ASME stress limits. To complement such paper, in the present article, different skirt geometry configurations are analyzed. The configurations here investigated consist of similar support skirts but with different angles of attachments between cylinder and cone parts. It will be possible to observe the influence of the bending stress in the collapse load and its relation to the allowable loads inferred from the ASME limits. A pressure vessel with torispherical head under internal pressure is also examined. Using elastic and limit load FEA, the present paper determines the collapse loads of the configurations. It sets up the relations between these collapse loads, stress categories, and limits dictated by the ASME Code Subsection NB. On the light of NB rules and philosophy, this paper shows how different methods of stress assessment, classification, and limits may influence in the design of a pressure vessel.« less

Design and fabrication considerations for stainless steel liquid helium jackets surrounding SCRF cavities

NASA Astrophysics Data System (ADS)

Bonnema, E. C.; Cunningham, E. K.; Rumel, J. D.

2014-01-01

The Department of Energy requires its subcontractors to meet 10 CFR 851 Appendix A Part 4 for all new pressure vessels and pressure piping. The stainless steel pressure vessel boundaries surrounding SCRF cavities fall under this requirement. Methods for meeting this requirement include design and fabrication of the pressure vessels to meet the requirements of the ASME Boiler & Pressure Vessel Code Section VIII Division 1 or Division 2. Design considerations include determining whether the configuration of the SCRF cavity can be accommodated under the rules of Division 1 or must be analyzed under Division 2 Part 4 Design by Rule Requirements or Part 5 Design by Analysis Requirements. Regardless of the Division or Part choice, designers will find the rules of the ASME Code require thicker pressure boundary members, larger welds, and additional non-destructive testing and quality assurance requirements. These challenges must be met and overcome by the fabricator through the development of robust, detailed, and repeatable manufacturing processes. In this paper we discuss the considerations for stainless steel pressure vessels that must meet the ASME Code and illustrate the discussion with examples from direct experience fabricating such vessels.

Technical Letter Report Development of Flaw Size Distribution Tables Including Effects of Flaw Depth Sizing Errors for Draft 10CFR 50.61a (Alternate PTS Rule) JCN-N6398, Task 4

DOE Office of Scientific and Technical Information (OSTI.GOV)

Simonen, Fredric A.; Gosselin, Stephen R.; Doctor, Steven R.

2013-04-22

This document describes a new method to determine whether the flaws in a particular reactor pressure vessel are consistent with the assumptions regarding the number and sizes of flaws used in the analyses that formed the technical justification basis for the new voluntary alternative Pressurized Thermal Shock (PTS) rule (Draft 10 CFR 50.61a). The new methodology addresses concerns regarding prior methodology because ASME Code Section XI examinations do not detect all fabrication flaws, they have higher detection performance for some flaw types, and there are flaw sizing errors always present (e.g., significant oversizing of small flaws and systematic under sizingmore » of larger flaws). The new methodology allows direct comparison of ASME Code Section XI examination results with values in the PTS draft rule Tables 2 and 3 in order to determine if the number and sizes of flaws detected by an ASME Code Section XI examination are consistent with those assumed in the probabilistic fracture mechanics calculations performed in support of the development of 10 CFR 50.61a.« less

Structural design, analysis, and code evaluation of an odd-shaped pressure vessel

NASA Astrophysics Data System (ADS)

Rezvani, M. A.; Ziada, H. H.

1992-12-01

An effort to design, analyze, and evaluate a rectangular pressure vessel is described. Normally pressure vessels are designed in circular or spherical shapes to prevent stress concentrations. In this case, because of operational limitations, the choice of vessels was limited to a rectangular pressure box with a removable cover plate. The American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code is used as a guideline for pressure containments whose width or depth exceeds 15.24 cm (6.0 in.) and where pressures will exceed 103.4 KPa (15.0 lbf/in(sup 2)). This evaluation used Section 8 of this Code, hereafter referred to as the Code. The dimensions and working pressure of the subject vessel fall within the pressure vessel category of the Code. The Code design guidelines and rules do not directly apply to this vessel. Therefore, finite-element methodology was used to analyze the pressure vessel, and the Code then was used in qualifying the vessel to be stamped to the Code. Section 8, Division 1 of the Code was used for evaluation. This action was justified by selecting a material for which fatigue damage would not be a concern. The stress analysis results were then checked against the Code, and the thicknesses adjusted to satisfy Code requirements. Although not directly applicable, the Code design formulas for rectangular vessels were also considered and presented.

Review Of Piping And Pressure Vessel Code Design Criteria. Technical Report 217.

DOE Office of Scientific and Technical Information (OSTI.GOV)

None, None

1969-04-18

This Technical Report summarizes a review of the design philosophies and criteria of the ASME Boiler and Pressure Vessel Code and the USASI Code for Pressure Piping. It traces the history of the Codes since their inception and critically reviews their present status. Recommendations are made concerning the applicability of the Codes to the special needs of LMFBR liquid sodium piping.

Analysis and Elimination of High Temperature Notch Induced Microcrack Initiation in Inconel 718 Nickel-Based Alloy

DTIC Science & Technology

1989-01-01

Compressor Rear Frame (ClF) which exhibits extensive cract:ing of the forward flange. ThL 1988 Actuarial Function data shows CRF crackiing As the number 2...Creep-Rupture properties of Waspaloy sheet to Sharp-Edged Notches in the Temperature Range of 1O000F-14O0OF. Journal of Basle Engineering, Trans ASME ...Dependence of the Notch Sensitivity of Waspaloy at 10000F-1400F on the Gamma Prime Phase, Journal of Basic Engineering, Trans ASME (in print at time of

D0 Silicon Upgrade: ASME Code and Pressure Calculations for Liquid Nitrogen Subcooler

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kuwazaki, Andrew; Leicht, Todd; /Fermilab

1995-10-04

Included in this engineering note are three separate calculation divisions. The first calculations are the determination of the required thickness of the LN{sub 2} subcooler flat head according to ASME code. This section includes Appendix A-C. The minimum plate thickness determined was 0.563 in. The actual thickness chosen in fabrication was a 3/4-inch plate milled to 0.594-inch at the bolt circle. Along with the plate thickness, this section calculates the required reinforcement area at the top plate penetrations. It was found that a 1/4-inch fillet weld at each penetration was adequate. The next set of calculations were done to provemore » that the subcooler internal pressure will always be less than 15 psig and therefore will not be classified as a pressure vessel. The subcooler is always open to a vent pipe. Appendix D calculations show that the vent pipe has a capacity of 1042 lbs/hr if 15 psig is present at the subcooler. It goes on to show that the inlet piping would at that flow rate, see a pressure drop of 104 psig. The maximum supply pressure of the LN{sub 2} storage dewar is 50 psig. Appendix E addresses required flow rates for steady state, loss of vacuum, or fire conditions. Page E9 shows a summary which states the maximum pressure would be 1.50 psig at fire conditions and internal pressure.« less

Alloy 740H Component Manufacturing Development

NASA Astrophysics Data System (ADS)

de Barbadillo, J. J.; Baker, B. A.; Gollihue, R. D.; Patel, S. J.

Alloy 740H was developed specifically for use in A-USC power plants. This alloy has been intensively evaluated in collaborative programs throughout the world, and the key properties have been verified and documented. In 2011 the alloy was approved for use in welded construction under ASME Code Case 2702. At present, alloy 740H is the only age-hardened nickel-base alloy that is ASME code approved. The emphasis for A-USC materials development is now on verification of the metalworking industry's capability to make the full range of mill product forms and sizes and to produce fittings and fabrications required for construction of a power plant. This paper presents the results of recent developments in component manufacture and evaluation.

40 CFR 89.3 - Acronyms and abbreviations.

Code of Federal Regulations, 2011 CFR

2011-07-01

... ASME American Society of Mechanical Engineers ASTM American Society for Testing and Materials CAA Clean... Oxygen OEM Original equipment manufacturer PM Particulate matter SAE Society of Automotive Engineers SEA...

40 CFR 89.3 - Acronyms and abbreviations.

Code of Federal Regulations, 2010 CFR

2010-07-01

... ASME American Society of Mechanical Engineers ASTM American Society for Testing and Materials CAA Clean... Oxygen OEM Original equipment manufacturer PM Particulate matter SAE Society of Automotive Engineers SEA...

Model building techniques for analysis.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walther, Howard P.; McDaniel, Karen Lynn; Keener, Donald

2009-09-01

The practice of mechanical engineering for product development has evolved into a complex activity that requires a team of specialists for success. Sandia National Laboratories (SNL) has product engineers, mechanical designers, design engineers, manufacturing engineers, mechanical analysts and experimentalists, qualification engineers, and others that contribute through product realization teams to develop new mechanical hardware. The goal of SNL's Design Group is to change product development by enabling design teams to collaborate within a virtual model-based environment whereby analysis is used to guide design decisions. Computer-aided design (CAD) models using PTC's Pro/ENGINEER software tools are heavily relied upon in the productmore » definition stage of parts and assemblies at SNL. The three-dimensional CAD solid model acts as the design solid model that is filled with all of the detailed design definition needed to manufacture the parts. Analysis is an important part of the product development process. The CAD design solid model (DSM) is the foundation for the creation of the analysis solid model (ASM). Creating an ASM from the DSM currently is a time-consuming effort; the turnaround time for results of a design needs to be decreased to have an impact on the overall product development. This effort can be decreased immensely through simple Pro/ENGINEER modeling techniques that summarize to the method features are created in a part model. This document contains recommended modeling techniques that increase the efficiency of the creation of the ASM from the DSM.« less

Acid Sphingomyelinase Gene Knockout Ameliorates Hyperhomocysteinemic Glomerular Injury in Mice Lacking Cystathionine-β-Synthase

PubMed Central

Boini, Krishna M.; Xia, Min; Abais, Justine M.; Xu, Ming; Li, Cai-xia; Li, Pin-Lan

2012-01-01

Acid sphingomyelinase (ASM) has been implicated in the development of hyperhomocysteinemia (hHcys)-induced glomerular oxidative stress and injury. However, it remains unknown whether genetically engineering of ASM gene produces beneficial or detrimental action on hHcys-induced glomerular injury. The present study generated and characterized the mice lacking cystathionine β-synthase (Cbs) and Asm mouse gene by cross breeding Cbs+/− and Asm+/− mice. Given that the homozygotes of Cbs−/−/Asm−/− mice could not survive for 3 weeks. Cbs+/−/Asm+/+, Cbs+/−/Asm+/− and Cbs+/−/Asm−/− as well as their Cbs wild type littermates were used to study the role of Asm−/− under a background of Cbs+/− with hHcys. HPLC analysis revealed that plasma Hcys level was significantly elevated in Cbs heterozygous (Cbs+/−) mice with different copies of Asm gene compared to Cbs+/+ mice with different Asm gene copies. Cbs+/−/Asm+/+ mice had significantly increased renal Asm activity, ceramide production and O2.− level compared to Cbs+/+/Asm+/+, while Cbs+/−/Asm−/− mice showed significantly reduced renal Asm activity, ceramide production and O2.− level due to increased plasma Hcys levels. Confocal microscopy demonstrated that colocalization of podocin with ceramide was much lower in Cbs+/−/Asm−/− mice compared to Cbs+/−/Asm+/+ mice, which was accompanied by a reduced glomerular damage index, albuminuria and proteinuria in Cbs+/−/Asm−/− mice. Immunofluorescent analyses of the podocin, nephrin and desmin expression also illustrated less podocyte damages in the glomeruli from Cbs+/−/Asm−/− mice compared to Cbs+/−/Asm+/+ mice. In in vitro studies of podocytes, hHcys-enhanced O2.− production, desmin expression, and ceramide production as well as decreases in VEGF level and podocin expression in podocytes were substantially attenuated by prior treatment with amitriptyline, an Asm inhibitor. In conclusion, Asm gene knockout or corresponding enzyme inhibition protects the podocytes and glomeruli from hHcys-induced oxidative stress and injury. PMID:23024785

A Survey of Variable Extragalactic Sources with XTE's All Sky Monitor (ASM)

NASA Technical Reports Server (NTRS)

Jernigan, Garrett

1998-01-01

The original goal of the project was the near real-time detection of AGN utilizing the SSC 3 of the ASM on XTE which does a deep integration on one 100 square degree region of the sky. While the SSC never performed sufficiently well to allow the success of this goal, the work on the project has led to the development of a new analysis method for coded aperture systems which has now been applied to ASM data for mapping regions near clusters of galaxies such as the Perseus Cluster and the Coma Cluster. Publications are in preparation that describe both the new method and the results from mapping clusters of galaxies.

Status and Outlook of Metric Conversion of Standards: The Views of Nine Selected Major Standards Development Bodies.

DTIC Science & Technology

1982-05-01

insufficient need for a hard metric version of the ASME Boiler and Pressure Vessel Code and industry would not support the metric version. The Code Is not...aircraft industry is concerned with certification requirements in metric units. The inch-pound Boiler and Pressure Vessel Code is the current standard

Effects of Cluster Porosity on the Tensile Properties of Butt-Weldments in T-1 Steel

DTIC Science & Technology

1974-11-01

i 12 Boiler and Pressure Vessel Code .19 In this code, the algebraic difference between the largest and smallest principal stresses is defined...Report U1LU- HN(J 7l-2()24 (University ot Illinois. 1971). "Nuclear Power Components.’* ASME Boiler and Pressure Vessel Code . Section HI. Subsections

10 CFR 50.55a - Codes and standards.

Code of Federal Regulations, 2010 CFR

2010-01-01

..., standard design approval, and standard design certification application under part 52 of this chapter is... section. (a)(1) Structures, systems, and components must be designed, fabricated, erected, constructed... Guide 1.84, Revision 34, “Design, Fabrication, and Materials Code Case Acceptability, ASME Section III...

Synchronizing Defense Transportation System Reference Files

DTIC Science & Technology

1998-02-01

and Pressure Vessel Code Federal Specification Military Specification BS AWS DIN JIS Food and Drug Administration Therapeutic Equivalence...269 270 276 278 279 280 282 287 288 289 292 293 294 296 299 300 301 302 307 308 309 311 312 314 Code source title ASME Boiler

Long term integrity for space station power systems

NASA Technical Reports Server (NTRS)

Leckie, F. A.; Marriott, D. L.

1991-01-01

A study was made of the High Temperature Design Codes ASME N47, British R5, and the French RCC-MR Rules. It is concluded that all these codes provide a good basis of design for space application. The new British R5 is the most complete since it deals with the problem of defects. The ASME N47 was subjected longer to practical application and scrutiny. A draft code is introduced, and a proposed draft for high temperature design in which attempts were made to identify gaps and improvements is suggested. The design is limited by creep characteristics. In these circumstances, life is strongly affected by the selected value of the factor of safety. The factor of safety of primary loads adopted in the codes is 1.5. Maybe a lower value of 1.25 is permissible for use in space. Long term creep rupture data for HAYNES 188 is deficient and it is suggested that extrapolation methods be investigated.

Pure Niobium as a Pressure Vessel Material

NASA Astrophysics Data System (ADS)

Peterson, T. J.; Carter, H. F.; Foley, M. H.; Klebaner, A. L.; Nicol, T. H.; Page, T. M.; Theilacker, J. C.; Wands, R. H.; Wong-Squires, M. L.; Wu, G.

2010-04-01

Physics laboratories around the world are developing niobium superconducting radio frequency (SRF) cavities for use in particle accelerators. These SRF cavities are typically cooled to low temperatures by direct contact with a liquid helium bath, resulting in at least part of the helium container being made from pure niobium. In the U.S., the Code of Federal Regulations allows national laboratories to follow national consensus pressure vessel rules or use of alternative rules which provide a level of safety greater than or equal to that afforded by ASME Boiler and Pressure Vessel Code. Thus, while used for its superconducting properties, niobium ends up also being treated as a material for pressure vessels. This report summarizes what we have learned about the use of niobium as a pressure vessel material, with a focus on issues for compliance with pressure vessel codes. We present results of a literature search for mechanical properties and tests results, as well as a review of ASME pressure vessel code requirements and issues.

The Effect of Cold Work on Properties of Alloy 617

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wright, Richard

2014-08-01

Alloy 617 is approved for non-nuclear construction in the ASME Boiler and Pressure Vessel Code Section I and Section VIII, but is not currently qualified for nuclear use in ASME Code Section III. A draft Code Case was submitted in 1992 to qualify the alloy for nuclear service but efforts were stopped before the approval process was completed.1 Renewed interest in high temperature nuclear reactors has resulted in a new effort to qualify Alloy 617 for use in nuclear pressure vessels. The mechanical and physical properties of Alloy 617 were extensively characterized for the VHTR programs in the 1980’s andmore » incorporated into the 1992 draft Code Case. Recently, the properties of modern heats of the alloy that incorporate an additional processing step, electro-slag re-melting, have been characterized both to confirm that the properties of contemporary material are consistent with those in the historical record and to increase the available database. A number of potential issues that were identified as requiring further consideration prior to the withdrawal of the 1992 Code Case are also being re-examined in the current R&D program. Code Cases are again being developed to allow use of Alloy 617 for nuclear design within the rules of the ASME Boiler and Pressure Vessel Code. In general the Code defines two temperature ranges for nuclear design with austenitic and nickel based alloys. Below 427°C (800°F) time dependent behavior is not considered, while above this temperature creep and creep-fatigue are considered to be the dominant life-limiting deformation modes. There is a corresponding differentiation in the treatment of the potential for effects associated with cold work. Below 427°C the principal issue is the relationship between the level of cold work and the propensity for stress corrosion cracking and above that temperature the primary concern is the impact of cold work on creep-rupture behavior.« less

Rotating Detonation Engine Research at NRL

DTIC Science & Technology

2013-07-01

Bykovskii, Wolanski, Falempin, Hayashi, Schauer, Yi, Wang, Brophy, Wu, Clafin, Smith, Tsuboi, Frolov, et al.) Recant RDE Studies at NRL Flow-field...Symposium) Injection/inflow effects (JPC 2011~044; ASM 2012-0617, ASM 2013-1178) the expansion region change RDE performance. Can this model be...Efficient Complex Configuration Simulation Capability 158 BASELINE SOLUTION • Basetine configUration Stoichiometric hydrogen-air RDE of Wolanski

46 CFR 56.01-5 - Adoption of ASME B31.1 for power piping, and other standards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... ENGINEERING PIPING SYSTEMS AND APPURTENANCES General § 56.01-5 Adoption of ASME B31.1 for power piping, and other standards. (a) Piping systems for ships and barges must be designed, constructed, and inspected in... subchapter. See 46 CFR 56.60-1(b) for the other adopted commercial standards applicable to piping systems...

DEVELOPMENT OF ASME SECTION X CODE RULES FOR HIGH PRESSURE COMPOSITE HYDROGEN PRESSURE VESSELS WITH NON-LOAD SHARING LINERS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rawls, G.; Newhouse, N.; Rana, M.

2010-04-13

The Boiler and Pressure Vessel Project Team on Hydrogen Tanks was formed in 2004 to develop Code rules to address the various needs that had been identified for the design and construction of up to 15000 psi hydrogen storage vessel. One of these needs was the development of Code rules for high pressure composite vessels with non-load sharing liners for stationary applications. In 2009, ASME approved new Appendix 8, for Section X Code which contains the rules for these vessels. These vessels are designated as Class III vessels with design pressure ranging from 20.7 MPa (3,000 ps)i to 103.4 MPamore » (15,000 psi) and maximum allowable outside liner diameter of 2.54 m (100 inches). The maximum design life of these vessels is limited to 20 years. Design, fabrication, and examination requirements have been specified, included Acoustic Emission testing at time of manufacture. The Code rules include the design qualification testing of prototype vessels. Qualification includes proof, expansion, burst, cyclic fatigue, creep, flaw, permeability, torque, penetration, and environmental testing.« less

Fatigue Behavior of HY-130 Steel Weldments Containing Fabrication Discontinuities.

DTIC Science & Technology

1985-04-18

discontinuities to solutions for elliptical discontinuities. One such approach has been formalized in the ASME Section XI Boiler and Pressure Vessel Code [1... Boiler and Pressure Vessel Code , Section XI, "Rules for Inservice Inspection of Nuclear Reactor Coolant Systems," American Society of Mechanical

Cryogenic distribution box for Fermi National Accelerator Laboratory

NASA Astrophysics Data System (ADS)

Svehla, M. R.; Bonnema, E. C.; Cunningham, E. K.

2017-12-01

Meyer Tool & Mfg., Inc (Meyer Tool) of Oak Lawn, Illinois is manufacturing a cryogenic distribution box for Fermi National Accelerator Laboratory (FNAL). The distribution box will be used for the Muon-to-electron conversion (Mu2e) experiment. The box includes twenty-seven cryogenic valves, two heat exchangers, a thermal shield, and an internal nitrogen separator vessel, all contained within a six-foot diameter ASME coded vacuum vessel. This paper discusses the design and manufacturing processes that were implemented to meet the unique fabrication requirements of this distribution box. Design and manufacturing features discussed include: 1) Thermal strap design and fabrication, 2) Evolution of piping connections to heat exchangers, 3) Nitrogen phase separator design, 4) ASME code design of vacuum vessel, and 5) Cryogenic valve installation.

46 CFR 59.01-2 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS... Engineering Standards (CG-521), 2100 2nd St., SW., Stop 7126, Washington, DC 20593-7126, and is available from the sources listed below. (b) American Society of Mechanical Engineers (ASME) International, Three...

46 CFR 59.01-2 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS... Engineering Standards (CG-ENG), 2100 2nd St., SW., Stop 7126, Washington, DC 20593-7126, and is available from the sources listed below. (b) American Society of Mechanical Engineers (ASME) International, Three...

46 CFR 59.01-2 - Incorporation by reference.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS...-ENG), Attn: Office of Design and Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther... below. (b) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York...

46 CFR 59.01-2 - Incorporation by reference.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS...-ENG), Attn: Office of Design and Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther... below. (b) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York...

46 CFR 59.01-2 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS... Engineering Standards (CG-521), 2100 2nd St., SW., Stop 7126, Washington, DC 20593-7126, and is available from the sources listed below. (b) American Society of Mechanical Engineers (ASME) International, Three...

Enhancing Decision Topology Assessment in Engineering Design

DTIC Science & Technology

2014-04-10

in engineering design decision making. References 1. Clemen , R. T., 1997, Making Hard Decisions, Second Edition, Duxbury Press. 2. Hazelrigg, G. A...United Kingdom. 6. Lewis, K., Chen, W. and L. C. Schmidt , Editors, 2006, Decision Making in Engineering Design, ASME Press, New York. 7. Myers

Mechanical Design and Analysis of LCLS II 2 K Cold Box

NASA Astrophysics Data System (ADS)

Yang, S.; Dixon, K.; Laverdure, N.; Rath, D.; Bevins, M.; Bai, H.; Kaminski, S.; Ravindranath, V.

2017-12-01

The mechanical design and analysis of the LCLS II 2 K cold box are presented. Its feature and functionality are discussed. ASME B31.3 was used to design its internal piping, and compliance of the piping code was ensured through flexibility analysis. The 2 K cold box was analyzed using ANSYS 17.2; the requirements of the applicable codes—ASME Section VIII Division 2 and ASCE 7-10—were satisfied. Seismic load was explicitly considered in both analyses.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arndt, T.E., Fluor Daniel Hanford

A previous evaluation documented in report WHC-SD-GN-RPT-30005, Rev. 0, titled ``Evaluation on Self-Contained High Efficiency Particulate Filters,`` revealed that the SCHEPA filters do not have required documentation to be in compliance with the design, testing, and fabrication standards required in ASME N-509, ASME N-510, and MIL-F-51068. These standards are required by DOE Order 6430.IA. Without this documentation, filter adequacy cannot be verified. The existing SCHEPA filters can be removed and replaced with new filters and filter housing which meet current codes and standards.

The Engineering Societies & Continuing Education.

ERIC Educational Resources Information Center

Professional Engineer, 1979

1979-01-01

Gives a description of what the major engineering societies (ASCE, ASME, AICHE, and IEEE) are doing in the area of continuing education. The description includes the short courses, their costs, duration, type and scope of the content. (GA)

ASME Material Challenges for Advanced Reactor Concepts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Piyush Sabharwall; Ali Siahpush

2013-07-01

This study presents the material Challenges associated with Advanced Reactor Concept (ARC) such as the Advanced High Temperature Reactor (AHTR). ACR are the next generation concepts focusing on power production and providing thermal energy for industrial applications. The efficient transfer of energy for industrial applications depends on the ability to incorporate cost-effective heat exchangers between the nuclear heat transport system and industrial process heat transport system. The heat exchanger required for AHTR is subjected to a unique set of conditions that bring with them several design challenges not encountered in standard heat exchangers. The corrosive molten salts, especially at highermore » temperatures, require materials throughout the system to avoid corrosion, and adverse high-temperature effects such as creep. Given the very high steam generator pressure of the supercritical steam cycle, it is anticipated that water tube and molten salt shell steam generators heat exchanger will be used. In this paper, the ASME Section III and the American Society of Mechanical Engineers (ASME) Section VIII requirements (acceptance criteria) are discussed. Also, the ASME material acceptance criteria (ASME Section II, Part D) for high temperature environment are presented. Finally, lack of ASME acceptance criteria for thermal design and analysis are discussed.« less

Potential Effects of Leak-Before-Break on Light Water Reactor Design.

DTIC Science & Technology

1985-08-26

Boiler and Pressure Vessel Code . In fact, section 3 of that code was created for nuclear applications. This... Boiler and Pressure Vessel Code . The only major change which leak-before-break would require in these analyses would be that all piping to be considered...XI of the ASME Boiler and Pressure Vessel Code , and is already required for all Class I piping systems in the plant. Class I systems are those

46 CFR 52.01-1 - Incorporation by reference.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther King Jr. Avenue SE., Washington, DC 20593...) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990...

46 CFR 52.01-1 - Incorporation by reference.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther King Jr. Avenue SE., Washington, DC 20593...) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990...

46 CFR 63.05-1 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY... is also available for inspection at the U.S. Coast Guard, Office of Design and Engineering Standards.../AGA Z21.22”), 63.25-3; and (2) [Reserved] (c) American Society of Mechanical Engineers (ASME...

49 CFR 178.338-3 - Structural integrity.

Code of Federal Regulations, 2010 CFR

2010-10-01

... calculated design stress at any point in the tank may not exceed the lesser of the maximum allowable stress... Code or the ASTM standard to which the material is manufactured. (3) The maximum design stress at any... ASME Code (IBR, see § 171.7 of this subchapter). The tank design must include calculation of stress due...

49 CFR 178.345-3 - Structural integrity.

Code of Federal Regulations, 2010 CFR

2010-10-01

... requirements and acceptance criteria. (1) The maximum calculated design stress at any point in the cargo tank wall may not exceed the maximum allowable stress value prescribed in Section VIII of the ASME Code (IBR... Code or the ASTM standard to which the material is manufactured. (3) The maximum design stress at any...

Analysis of On-Board Oxygen and Nitrogen Generation Systems for Surface Vessels.

DTIC Science & Technology

1983-06-01

and Pressure Vessel Code SAE AIR 822 Oxygen for General Aviation Aircraft SAE AIR 825 Oxygen for Aircrafts SAE AIR 1059 Transportation and Maintenance...OF THE TITLE MIL-T-27730 Threaded Components MIL-P-27401 A 40 Micron Filter For Nitrogen MIL-V-33650 Internal Straight Threads ASME Code VIII Boiler

49 CFR 178.345-3 - Structural integrity.

Code of Federal Regulations, 2011 CFR

2011-10-01

... acceptance criteria. (1) The maximum calculated design stress at any point in the cargo tank wall may not exceed the maximum allowable stress value prescribed in Section VIII of the ASME Code (IBR, see § 171.7... Code or the ASTM standard to which the material is manufactured. (3) The maximum design stress at any...

Allele-Specific Methylation Occurs at Genetic Variants Associated with Complex Disease

PubMed Central

Hutchinson, John N.; Raj, Towfique; Fagerness, Jes; Stahl, Eli; Viloria, Fernando T.; Gimelbrant, Alexander; Seddon, Johanna; Daly, Mark; Chess, Andrew; Plenge, Robert

2014-01-01

We hypothesize that the phenomenon of allele-specific methylation (ASM) may underlie the phenotypic effects of multiple variants identified by Genome-Wide Association studies (GWAS). We evaluate ASM in a human population and document its genome-wide patterns in an initial screen at up to 380,678 sites within the genome, or up to 5% of the total genomic CpGs. We show that while substantial inter-individual variation exists, 5% of assessed sites show evidence of ASM in at least six samples; the majority of these events (81%) are under genetic influence. Many of these cis-regulated ASM variants are also eQTLs in peripheral blood mononuclear cells and monocytes and/or in high linkage-disequilibrium with variants linked to complex disease. Finally, focusing on autoimmune phenotypes, we extend this initial screen to confirm the association of cis-regulated ASM with multiple complex disease-associated variants in an independent population using next-generation bisulfite sequencing. These four variants are implicated in complex phenotypes such as ulcerative colitis and AIDS progression disease (rs10491434), Celiac disease (rs2762051), Crohn's disease, IgA nephropathy and early-onset inflammatory bowel disease (rs713875) and height (rs6569648). Our results suggest cis-regulated ASM may provide a mechanistic link between the non-coding genetic changes and phenotypic variation observed in these diseases and further suggests a route to integrating DNA methylation status with GWAS results. PMID:24911414

46 CFR 52.01-1 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... inspection at the U.S. Coast Guard, Office of Design and Engineering Standards (CG-ENG), 2100 2nd St., SW... Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) 2001...

46 CFR 63.05-1 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY... is also available for inspection at the U.S. Coast Guard, Office of Design and Engineering Standards... (“ANSI/AGA Z21.22”), 63.25-3; and (2) [Reserved] (c) American Society of Mechanical Engineers (ASME...

46 CFR 52.01-1 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... inspection at the U.S. Coast Guard, Office of Design and Engineering Standards (CG-521), 2100 2nd St., SW... Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) 2001...

46 CFR 52.01-1 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... inspection at the U.S. Coast Guard, Office of Design and Engineering Standards (CG-521), 2100 2nd St., SW... Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) 2001...

46 CFR 63.05-1 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY... is also available for inspection at the U.S. Coast Guard, Office of Design and Engineering Standards... (“ANSI/AGA Z21.22”), 63.25-3; and (2) [Reserved] (c) American Society of Mechanical Engineers (ASME...

Active State Model for Autonomous Systems

NASA Technical Reports Server (NTRS)

Park, Han; Chien, Steve; Zak, Michail; James, Mark; Mackey, Ryan; Fisher, Forest

2003-01-01

The concept of the active state model (ASM) is an architecture for the development of advanced integrated fault-detection-and-isolation (FDI) systems for robotic land vehicles, pilotless aircraft, exploratory spacecraft, or other complex engineering systems that will be capable of autonomous operation. An FDI system based on the ASM concept would not only provide traditional diagnostic capabilities, but also integrate the FDI system under a unified framework and provide mechanism for sharing of information between FDI subsystems to fully assess the overall health of the system. The ASM concept begins with definitions borrowed from psychology, wherein a system is regarded as active when it possesses self-image, self-awareness, and an ability to make decisions itself, such that it is able to perform purposeful motions and other transitions with some degree of autonomy from the environment. For an engineering system, self-image would manifest itself as the ability to determine nominal values of sensor data by use of a mathematical model of itself, and selfawareness would manifest itself as the ability to relate sensor data to their nominal values. The ASM for such a system may start with the closed-loop control dynamics that describe the evolution of state variables. As soon as this model was supplemented with nominal values of sensor data, it would possess self-image. The ability to process the current sensor data and compare them with the nominal values would represent self-awareness. On the basis of self-image and self-awareness, the ASM provides the capability for self-identification, detection of abnormalities, and self-diagnosis.

ASME Section VIII Recertification of a 33,000 Gallon Vacuum-jacketed LH2 Storage Vessel for Densified Hydrogen Testing at NASA Kennedy Space Center

NASA Technical Reports Server (NTRS)

Swanger, Adam M.; Notardonato, William U.; Jumper, Kevin M.

2015-01-01

The Ground Operations Demonstration Unit for Liquid Hydrogen (GODU-LH2) has been developed at NASA Kennedy Space Center in Florida. GODU-LH2 has three main objectives: zero-loss storage and transfer, liquefaction, and densification of liquid hydrogen. A cryogenic refrigerator has been integrated into an existing, previously certified, 33,000 gallon vacuum-jacketed storage vessel built by Minnesota Valley Engineering in 1991 for the Titan program. The dewar has an inner diameter of 9.5 and a length of 71.5; original design temperature and pressure ranges are -423 F to 100 F and 0 to 95 psig respectively. During densification operations the liquid temperature will be decreased below the normal boiling point by the refrigerator, and consequently the pressure inside the inner vessel will be sub-atmospheric. These new operational conditions rendered the original certification invalid, so an effort was undertaken to recertify the tank to the new pressure and temperature requirements (-12.7 to 95 psig and -433 F to 100 F respectively) per ASME Boiler and Pressure Vessel Code, Section VIII, Division 1. This paper will discuss the unique design, analysis and implementation issues encountered during the vessel recertification process.

Methodology for the structural design of single spoke accelerating cavities at Fermilab

DOE PAGES

Passarelli, Donato; Wands, Robert H.; Merio, Margherita; ...

2016-10-01

Fermilab is planning to upgrade its accelerator complex to deliver a more powerful and intense proton-beam for neutrino experiments. In the framework of the so-called Proton Improvement Plan-II (PIP-II), we are designing and developing a cryomodule containing superconducting accelerating cavities, the Single Spoke Resonators of type 1 (SSR1). In this paper, we present the sequence of analysis and calculations performed for the structural de- sign of these cavities, using the rules of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code (BPVC). The lack of an accepted procedure for addressing the design, fabrication, and inspection of suchmore » unique pressure vessels makes the task demanding and challenging every time. Several factors such as exotic materials, unqualified brazing procedures, limited nondestructive examination, and the general R&D nature of these early generations of cavity design, conspire to make it impractical to obtain full compliance with all ASME BPVC requirements. However, the presented approach allowed us to validate the design of these new generation of single spoke cavities with values of maximum allowable working pressure that exceed the safety requirements. This set of rules could be used as a starting point for the structural design and development of similar objects.« less

Methodology for the structural design of single spoke accelerating cavities at Fermilab

NASA Astrophysics Data System (ADS)

Passarelli, Donato; Wands, Robert H.; Merio, Margherita; Ristori, Leonardo

2016-10-01

Fermilab is planning to upgrade its accelerator complex to deliver a more powerful and intense proton-beam for neutrino experiments. In the framework of the so-called Proton Improvement Plan-II (PIP-II), we are designing and developing a cryomodule containing superconducting accelerating cavities, the Single Spoke Resonators of type 1 (SSR1). In this paper, we present the sequence of analysis and calculations performed for the structural design of these cavities, using the rules of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code (BPVC). The lack of an accepted procedure for addressing the design, fabrication, and inspection of such unique pressure vessels makes the task demanding and challenging every time. Several factors such as exotic materials, unqualified brazing procedures, limited nondestructive examination, and the general R&D nature of these early generations of cavity design, conspire to make it impractical to obtain full compliance with all ASME BPVC requirements. However, the presented approach allowed us to validate the design of this new generation of single spoke cavities with values of maximum allowable working pressure that exceeds the safety requirements. This set of rules could be used as a starting point for the structural design and development of similar objects.

Fluid Dynamic - Structural Interactions of Labyrinth Seals.

DTIC Science & Technology

1986-08-01

A., "The Leakage of Steam Through Labyrinth Seals ", Trans. ASME, Vol. 57, 1935, pp 115-122. 21. Komotori, K., "A Consideration on the Labyrinth ...October 1980. 17. Vermes, G., "A fluid-Mechanics Approach to the Labyrinth Seal Leakage Problem", Journal of Basic Engineering, Tr. ASME, Series D...INTERACTIONS OF LABYRINTH SEALS Manuel Martinez-Sanchez John Dugundji Gas Turbine and Plasma Dynamics Laboratory D T IC Department of Aeronautics and

Comparative anatomy of chromosomal domains with imprinted and non-imprinted allele-specific DNA methylation.

PubMed

Paliwal, Anupam; Temkin, Alexis M; Kerkel, Kristi; Yale, Alexander; Yotova, Iveta; Drost, Natalia; Lax, Simon; Nhan-Chang, Chia-Ling; Powell, Charles; Borczuk, Alain; Aviv, Abraham; Wapner, Ronald; Chen, Xiaowei; Nagy, Peter L; Schork, Nicholas; Do, Catherine; Torkamani, Ali; Tycko, Benjamin

2013-08-01

Allele-specific DNA methylation (ASM) is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons), one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault RNA in band 5q31, proved to be new examples of imprinted DMRs (maternal alleles methylated) while a third, between STEAP3 and C2orf76 in chromosome band 2q14, showed non-imprinted haplotype-dependent ASM. Using long-read bisulfite sequencing (bis-seq) in 8 human tissues we found that in all 3 domains the ASM is restricted to single differentially methylated regions (DMRs), each less than 2kb. The ASM in the C3orf27-RPN1 intergenic region was placenta-specific and associated with allele-specific expression of a long non-coding RNA. Strikingly, the discrete DMRs in all 3 regions overlap with binding sites for the insulator protein CTCF, which we found selectively bound to the unmethylated allele of the STEAP3-C2orf76 DMR. Methylation mapping in two additional genes with non-imprinted haplotype-dependent ASM, ELK3 and CYP2A7, showed that the CYP2A7 DMR also overlaps a CTCF site. Thus, two features of imprinted domains, highly localized DMRs and allele-specific insulator occupancy by CTCF, can also be found in chromosomal domains with non-imprinted ASM. Arguing for biological importance, our analysis of published whole genome bis-seq data from hES cells revealed multiple genome-wide association study (GWAS) peaks near CTCF binding sites with ASM.

Comparative Anatomy of Chromosomal Domains with Imprinted and Non-Imprinted Allele-Specific DNA Methylation

PubMed Central

Kerkel, Kristi; Yale, Alexander; Yotova, Iveta; Drost, Natalia; Lax, Simon; Nhan-Chang, Chia-Ling; Powell, Charles; Borczuk, Alain; Aviv, Abraham; Wapner, Ronald; Chen, Xiaowei; Nagy, Peter L.; Schork, Nicholas; Do, Catherine; Torkamani, Ali; Tycko, Benjamin

2013-01-01

Allele-specific DNA methylation (ASM) is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons), one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault RNA in band 5q31, proved to be new examples of imprinted DMRs (maternal alleles methylated) while a third, between STEAP3 and C2orf76 in chromosome band 2q14, showed non-imprinted haplotype-dependent ASM. Using long-read bisulfite sequencing (bis-seq) in 8 human tissues we found that in all 3 domains the ASM is restricted to single differentially methylated regions (DMRs), each less than 2kb. The ASM in the C3orf27-RPN1 intergenic region was placenta-specific and associated with allele-specific expression of a long non-coding RNA. Strikingly, the discrete DMRs in all 3 regions overlap with binding sites for the insulator protein CTCF, which we found selectively bound to the unmethylated allele of the STEAP3-C2orf76 DMR. Methylation mapping in two additional genes with non-imprinted haplotype-dependent ASM, ELK3 and CYP2A7, showed that the CYP2A7 DMR also overlaps a CTCF site. Thus, two features of imprinted domains, highly localized DMRs and allele-specific insulator occupancy by CTCF, can also be found in chromosomal domains with non-imprinted ASM. Arguing for biological importance, our analysis of published whole genome bis-seq data from hES cells revealed multiple genome-wide association study (GWAS) peaks near CTCF binding sites with ASM. PMID:24009515

Creep and Creep-Fatigue Crack Growth at Structural Discontinuities and Welds

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dr. F. W. Brust; Dr. G. M. Wilkowski; Dr. P. Krishnaswamy

2010-01-27

The subsection ASME NH high temperature design procedure does not admit crack-like defects into the structural components. The US NRC identified the lack of treatment of crack growth within NH as a limitation of the code and thus this effort was undertaken. This effort is broken into two parts. Part 1, summarized here, involved examining all high temperature creep-fatigue crack growth codes being used today and from these, the task objective was to choose a methodology that is appropriate for possible implementation within NH. The second part of this task, which has just started, is to develop design rules formore » possible implementation within NH. This second part is a challenge since all codes require step-by-step analysis procedures to be undertaken in order to assess the crack growth and life of the component. Simple rules for design do not exist in any code at present. The codes examined in this effort included R5, RCC-MR (A16), BS 7910, API 579, and ATK (and some lesser known codes). There are several reasons that the capability for assessing cracks in high temperature nuclear components is desirable. These include: (1) Some components that are part of GEN IV reactors may have geometries that have sharp corners - which are essentially cracks. Design of these components within the traditional ASME NH procedure is quite challenging. It is natural to ensure adequate life design by modeling these features as cracks within a creep-fatigue crack growth procedure. (2) Workmanship flaws in welds sometimes occur and are accepted in some ASME code sections. It can be convenient to consider these as flaws when making a design life assessment. (3) Non-destructive Evaluation (NDE) and inspection methods after fabrication are limited in the size of the crack or flaw that can be detected. It is often convenient to perform a life assessment using a flaw of a size that represents the maximum size that can elude detection. (4) Flaws that are observed using in-service detection methods often need to be addressed as plants age. Shutdown inspection intervals can only be designed using creep and creep-fatigue crack growth techniques. (5) The use of crack growth procedures can aid in examining the seriousness of creep damage in structural components. How cracks grow can be used to assess margins on components and lead to further safe operation. After examining the pros and cons of all these methods, the R5 code was chosen as the most up-to-date and validated high temperature creep and creep fatigue code currently used in the world at present. R5 is considered the leader because the code: (1) has well established and validated rules, (2) has a team of experts continually improving and updating it, (3) has software that can be used by designers, (4) extensive validation in many parts with available data from BE resources as well as input from Imperial college's database, and (5) was specifically developed for use in nuclear plants. R5 was specifically developed for use in gas cooled nuclear reactors which operate in the UK and much of the experience is based on materials and temperatures which are experienced in these reactors. If the next generation advanced reactors to be built in the US used these same materials within the same temperature ranges as these reactors, then R5 may be appropriate for consideration of direct implementation within ASME code NH or Section XI. However, until more verification and validation of these creep/fatigue crack growth rules for the specific materials and temperatures to be used in the GEN IV reactors is complete, ASME should consider delaying this implementation. With this in mind, it is this authors opinion that R5 methods are the best available for code use today. The focus of this work was to examine the literature for creep and creep-fatigue crack growth procedures that are well established in codes in other countries and choose a procedure to consider implementation into ASME NH. It is very important to recognize that all creep and creep fatigue crack growth procedures that are part of high temperature design codes are related and very similar. This effort made no attempt to develop a new creep-fatigue crack growth predictive methodology. Rather examination of current procedures was the only goal. The uncertainties in the R5 crack growth methods and recommendations for more work are summarized here also.« less

46 CFR 64.2 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Incorporation by reference. 64.2 Section 64.2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING MARINE PORTABLE TANKS AND... sections affected, are: American Society of Mechanical Engineers (ASME) International Three Park Avenue...

Development and characterization of a 3D multicell microtissue culture model of airway smooth muscle.

PubMed

West, Adrian R; Zaman, Nishat; Cole, Darren J; Walker, Matthew J; Legant, Wesley R; Boudou, Thomas; Chen, Christopher S; Favreau, John T; Gaudette, Glenn R; Cowley, Elizabeth A; Maksym, Geoffrey N

2013-01-01

Airway smooth muscle (ASM) cellular and molecular biology is typically studied with single-cell cultures grown on flat 2D substrates. However, cells in vivo exist as part of complex 3D structures, and it is well established in other cell types that altering substrate geometry exerts potent effects on phenotype and function. These factors may be especially relevant to asthma, a disease characterized by structural remodeling of the airway wall, and highlights a need for more physiologically relevant models of ASM function. We utilized a tissue engineering platform known as microfabricated tissue gauges to develop a 3D culture model of ASM featuring arrays of ∼0.4 mm long, ∼350 cell "microtissues" capable of simultaneous contractile force measurement and cell-level microscopy. ASM-only microtissues generated baseline tension, exhibited strong cellular organization, and developed actin stress fibers, but lost structural integrity and dissociated from the cantilevers within 3 days. Addition of 3T3-fibroblasts dramatically improved survival times without affecting tension development or morphology. ASM-3T3 microtissues contracted similarly to ex vivo ASM, exhibiting reproducible responses to a range of contractile and relaxant agents. Compared with 2D cultures, microtissues demonstrated identical responses to acetylcholine and KCl, but not histamine, forskolin, or cytochalasin D, suggesting that contractility is regulated by substrate geometry. Microtissues represent a novel model for studying ASM, incorporating a physiological 3D structure, realistic mechanical environment, coculture of multiple cells types, and comparable contractile properties to existing models. This new model allows for rapid screening of biochemical and mechanical factors to provide insight into ASM dysfunction in asthma.

Ocean Engineering Studies Compiled 1991. Volume 7. Acrylic Windows- Diverse Design Features and Types of Service

DTIC Science & Technology

1991-01-01

acrylic plastic windows in chambers for human occupancy, the design stress should not exceed 800 psi (i.e., conversion factor of 20). 3. installed...pressure vessels for human occupancy Is 10 yr based on the conserva- tive assumption that in that length of time even stressed acrylic in a tropioal...the Safety Standard for Pressure Ves- sels for Human Oc-upancy (ASME PVHO-1 Safety Standard). Since that time, this ASME Safety Standard has

46 CFR 56.95-1 - General (replaces 136).

Code of Federal Regulations, 2013 CFR

2013-10-01

... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

46 CFR 56.95-1 - General (replaces 136).

Code of Federal Regulations, 2010 CFR

2010-10-01

... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

46 CFR 56.95-1 - General (replaces 136).

Code of Federal Regulations, 2014 CFR

2014-10-01

... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

46 CFR 56.95-1 - General (replaces 136).

Code of Federal Regulations, 2012 CFR

2012-10-01

... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

46 CFR 56.95-1 - General (replaces 136).

Code of Federal Regulations, 2011 CFR

2011-10-01

... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

Traditional engineering in the biological century: the biotraditional engineer.

PubMed

Friedman, M H

2001-12-01

The increasing importance of life science in all engineering is prompting departments in the traditional engineering disciplines to offer life science as part of their curricula. Students who take advantage of this opportunity--"biotraditional engineers"--will be well positioned for careers in their discipline and in related areas of bioengineering. The founder engineering societies, such as the Bioengineering Division of ASME, are responding to this trend by broadening their scope and working increasingly across interdisciplinary borders.

46 CFR 54.10-5 - Maximum allowable working pressure (reproduces UG-98).

Code of Federal Regulations, 2010 CFR

2010-10-01

... section VIII of the ASME Boiler and Pressure Vessel Code, together with the effect of any combination of... operating temperature, using for each temperature the applicable allowable stress value. Note: Table 54.10-5...

WaterSense Specification for Showerheads Supporting Statement

EPA Pesticide Factsheets

WaterSense collaborated with the American Society of Mechanical Engineers (ASME)/Canadian Standards Association (CSA) Joint Harmonization Task Force to develop the specification criteria for high-efficiency showerheads.

40 CFR 98.444 - Monitoring and QA/QC requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

...), the American Gas Association (AGA), the American Society of Mechanical Engineers (ASME), the American... any flow meter calibrations performed are National Institute of Standards and Technology (NIST...

Application of materials database (MAT.DB.) to materials education

NASA Technical Reports Server (NTRS)

Liu, Ping; Waskom, Tommy L.

1994-01-01

Finding the right material for the job is an important aspect of engineering. Sometimes the choice is as fundamental as selecting between steel and aluminum. Other times, the choice may be between different compositions in an alloy. Discovering and compiling materials data is a demanding task, but it leads to accurate models for analysis and successful materials application. Mat. DB. is a database management system designed for maintaining information on the properties and processing of engineered materials, including metals, plastics, composites, and ceramics. It was developed by the Center for Materials Data of American Society for Metals (ASM) International. The ASM Center for Materials Data collects and reviews material property data for publication in books, reports, and electronic database. Mat. DB was developed to aid the data management and material applications.

Management Sciences Division Annual Report (9th)

DTIC Science & Technology

1992-01-01

41 Actuarial Process Consolidation and Review ....................................... 43 How M alfunction Code Reduction...47 Sun W ork Stations ............................................................................... 48 Actuarial Process Consolidation and...Information System (WSMIS). Dyna-METRIC is used for wartime supply support capability assessments. The Aircraft Sustainability Model ( ASM ) is the

Detailed Drawings for the Force Balance Test Apparatus

EPA Pesticide Factsheets

The American Society of Mechanical Engineers (ASME)/Canadian Standards Association (CSA) Joint Harmonization Task Force on water-efficient showerheads used the force balance test apparatus shown in these drawings.

Viscosity of diesel engine fuel oil under pressure

NASA Technical Reports Server (NTRS)

Hersey, Mayo D

1929-01-01

In the development of Diesel engine fuel injection systems it is necessary to have an approximate knowledge of the absolute viscosity of the fuel oil under high hydrostatic pressures. This report presents the results of experimental tests conducted by Mr. Jackson Newton Shore, utilizing the A.S.M.E. high pressure equipment.

78 FR 20141 - Notice Pursuant to the National Cooperative Research and Production Act of 1993 -- American...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-03

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993 -- American Society of Mechanical Engineers Notice is hereby given that, on March 5....C. 4301 et seq. (``the Act''), the American Society of Mechanical Engineers (``ASME'') has filed...

78 FR 58558 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-24

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993--American Society of Mechanical Engineers Notice is hereby given that, on August 20....C. 4301 et seq. (``the Act''), the American Society of Mechanical Engineers (``ASME'') has filed...

76 FR 80406 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-23

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993--American Society of Mechanical Engineers Notice is hereby given that, on December 6....C. 4301 et seq. (``the Act''), American Society of Mechanical Engineers (``ASME'') has filed written...

76 FR 27351 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-11

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993--American Society of Mechanical Engineers Notice is hereby given that, on April 12....C. 4301 et seq. (``the Act''), American Society Of Mechanical Engineers (``ASME'') has filed written...

75 FR 70031 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-16

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993--American Society of Mechanical Engineers Notice is hereby given that, on October 14....C. 4301 et seq. (``the Act''), American Society of Mechanical Engineers (``ASME'') has filed written...

76 FR 52014 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-19

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993--American Society of Mechanical Engineers Notice is hereby given that, on July 25....C. 4301 et seq. (``the Act''), American Society of Mechanical Engineers (``ASME'') has filed written...

76 FR 6497 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-04

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993--American Society of Mechanical Engineers Notice is hereby given that, on January 10....C. 4301 et seq. (``the Act''), American Society of Mechanical Engineers (``ASME'') has filed written...

75 FR 45156 - Notice Pursuant to the National Cooperative Research and Production Act of 1993; American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-02

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993; American Society of Mechanical Engineers Notice is hereby given that, on June 28....C. 4301 et seq. (``the Act''), the American Society of Mechanical Engineers (``ASME'') has filed...

77 FR 31041 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-24

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993--American Society of Mechanical Engineers Notice is hereby given that, on April 27....C. 4301 et seq. (``the Act''), the American Society of Mechanical Engineers (``ASME'') has filed...

75 FR 14191 - Notice Pursuant to The National Cooperative Research and Production Act of 1993-American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-24

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to The National Cooperative Research and Production Act of 1993--American Society of Mechanical Engineers Notice is hereby given that, on February 25....C. 4301 et seq.. (``the Act''), the American Society of Mechanical Engineers (``ASME'') has filed...

77 FR 58412 - Notice Pursuant to the National Cooperative Research and Production Act of 1993; American Society...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-20

... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and Production Act of 1993; American Society of Mechanical Engineers Notice is hereby given that, on August 27....C. 4301 et seq. (``the Act''), the American Society of Mechanical Engineers (``ASME'') has filed...

The role of the AWS CWI in a major power plant outage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walsh, T.W.

In March of 1988, an eight-week outage began at the Keystone Electric Generating Station, 35 miles northwest of Johnstown, Pa. This outage encompassed the complete replacement of the reheat section of a 1,7000,000 kW boiler unit, as well as major repairs to the boiler itself. The author discusses how AWS (Certified Welding Inspectors) (CWl's) played a major part in the successful completion of over 7600 ASME butt joint welds during the outage. The welding on these outages is performed in strict accordance with the Pennsylvania Electric Company Quanity Assurance Specification and the applicable codes (ASME, NBIC, ANSI and AWS).

The Engaged Microbiologist: Bringing the Microbiological Sciences to the K-12 Community.

PubMed

Westenberg, David J

2016-03-01

Exposing K-12 students to cutting edge science that impacts their daily lives can bring classroom lessons to life. Citizen-science projects are an excellent way to bring high-level science to the classroom and help satisfy one of the cornerstone concepts of the Next Generation Science Standards (NGSS), "engaging in practices that scientists and engineers actually use." This can be a daunting task for teachers who may lack the background or resources to integrate these projects into the classroom. This is where scientific societies such as the American Society for Microbiology (ASM) can play a critical role. ASM encourages its members to engage with the K-12 community by providing networking opportunities and resources for ASM members and K-12 teachers to work together to bring microbiology into the classroom. Journal of Microbiology & Biology Education.

Report on FY15 Alloy 617 SMT Creep-Fatigue Test Results

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Yanli; Jetter, Robert I.; Baird, Seth T.

For the temperature range of 990-950C, Alloy 617 is a candidate IHX structural material for high temperature gas reactors (HTGRs) because of its high temperature creep properties. Also, its superior strength over a broad temperature range also offers advantages for certain component applications. In order for the designers to be able to use Alloy 617 for these high temperature components, Alloy 617 has to be approved for use in Section III (the nuclear section) of the ASME (American Society of Mechanical Engineers) Boiler and Pressure Vessel Code. A plan has been developed to propose a Code Case for use ofmore » Alloy 617 at elevated temperature in Section III of the ASME Code by September 2015. There has not been a new high temperature material approved for use in Section III for almost 20 years. The Alloy 617 Code Case effort would lead the way to establish a path for Code qualification of new high temperature materials of interest to other advanced SMRs. Creep-fatigue at elevated temperatures is the most damaging structural failure mode. In the past 40 years significant efforts have been devoted to the elevated temperature Code rule development in Section III, Subsection NH* of the ASME Boiler and Pressure Vessel Code, to ascertain conservative structural designs to prevent creep-fatigue failure. The current Subsection NH creep-fatigue procedure was established by the steps of (1) analytically obtaining a detailed stress-strain history, (2) comparing the stress and strain components to cyclic test results deconstructed into stress and strain quantities, and (3) recombining the results to obtain a damage function in the form of the so-called creep-fatigue damage-diagram. The deconstruction and recombination present difficulties in evaluation of test data and determination of cyclic damage in design. The uncertainties in these steps lead to the use of overly conservative design factors in the current creep-fatigue procedure. In addition, and of major significance to the viability of the Alloy 617 Code Case, the use of the current elastic analysis based rules in Subsection NH for the evaluation of strain limits (a precursor for the creep-fatigue rules) and the creep-fatigue rules themselves have been deemed inappropriate for Alloy 617 at temperatures above 650C (Corum and Brass, 1991). The rationale for this exclusion is that at higher temperatures it is not feasible to decouple plasticity and creep, which is the basis for the current simplified rules. This temperature, 650C, is well below the temperature range of interest for this material for the High Temperature Gas Cooled Reactor (HTGR) as well as the VHTR. The only current alternative is, thus, a full inelastic analysis which requires sophisticated material models which have not yet been formulated and verified. To address the prohibition on the use of current methods at very high temperatures, proposed Code rules have been developed which are based on the use of elastic-perfectly plastic (E-PP) analysis methods and which are expected to be applicable to very high temperatures. To provide data to implement the proposed rules and to verify their application, a series of tests have been initiated. One test concept, the Simplified Model Test (SMT), takes into account the stress and strain redistribution in real structures by including representative follow-up characteristics in the test specimen. The correlation parameter between test and design is the elastically calculated strain, and the dependent test variable is the observed cycles to failure. Although the initial priority for the SMT approach is to generate data to support validation of the E-PP Code Case for evaluation of creep-fatigue damage, the broader goal of the SMT approach is to develop a methodology for evaluation of creep fatigue damage which is simpler to implement than the current complex rules and applicable to the full temperature range from ambient conditions to the very high temperature creep regime of 900-950C. Also, guidance has been received from ASME Code committees that the proposed EPP methodology for evaluation of creep-fatigue damage should be extended to the other Subsection NH materials to the extent feasible. Thus, the scope of testing has been expanded to include SS304H and SS316H. This report describes the SMT approach and the development of testing capability to conduct SMT experiments on Alloy 617 and 304H and 316H and stainless steels. These SMT specimen data are also representative of component loading conditions and have been used as part of the verification of the proposed elastic-perfectly plastic Code Cases. Results from the SMT tests on both Alloy 617 and SS316H were compared to the predictions from the EPP Creep-Fatigue Code Case. Two different comparisons were made; one based on design life equal to the test duration and the other with an acceptable design life determined from the EPP Code Case procedure. The latter approach permits the determination of...« less

Progressive Failure And Life Prediction of Ceramic and Textile Composites

NASA Technical Reports Server (NTRS)

Xue, David Y.; Shi, Yucheng; Katikala, Madhu; Johnston, William M., Jr.; Card, Michael F.

1998-01-01

An engineering approach to predict the fatigue life and progressive failure of multilayered composite and textile laminates is presented. Analytical models which account for matrix cracking, statistical fiber failures and nonlinear stress-strain behavior have been developed for both composites and textiles. The analysis method is based on a combined micromechanics, fracture mechanics and failure statistics analysis. Experimentally derived empirical coefficients are used to account for the interface of fiber and matrix, fiber strength, and fiber-matrix stiffness reductions. Similar approaches were applied to textiles using Repeating Unit Cells. In composite fatigue analysis, Walker's equation is applied for matrix fatigue cracking and Heywood's formulation is used for fiber strength fatigue degradation. The analysis has been compared with experiment with good agreement. Comparisons were made with Graphite-Epoxy, C/SiC and Nicalon/CAS composite materials. For textile materials, comparisons were made with triaxial braided and plain weave materials under biaxial or uniaxial tension. Fatigue predictions were compared with test data obtained from plain weave C/SiC materials tested at AS&M. Computer codes were developed to perform the analysis. Composite Progressive Failure Analysis for Laminates is contained in the code CPFail. Micromechanics Analysis for Textile Composites is contained in the code MicroTex. Both codes were adapted to run as subroutines for the finite element code ABAQUS and CPFail-ABAQUS and MicroTex-ABAQUS. Graphic user interface (GUI) was developed to connect CPFail and MicroTex with ABAQUS.

Shuttle Systems 3-D Applications: Application of 3-D Graphics in Engineering Training for Shuttle Ground Processing

NASA Technical Reports Server (NTRS)

Godfrey, Gary S.

2003-01-01

This project illustrates an animation of the orbiter mate to the external tank, an animation of the OMS POD installation to the orbiter, and a simulation of the landing gear mechanism at the Kennedy Space Center. A detailed storyboard was created to reflect each animation or simulation. Solid models were collected and translated into Pro/Engineer's prt and asm formats. These solid models included computer files of the: orbiter, external tank, solid rocket booster, mobile launch platform, transporter, vehicle assembly building, OMS POD fixture, and landing gear. A depository of the above solid models was established. These solid models were translated into several formats. This depository contained the following files: stl for sterolithography, stp for neutral file work, shrinkwrap for compression, tiff for photoshop work, jpeg for Internet use, and prt and asm for Pro/Engineer use. Solid models were created of the material handling sling, bay 3 platforms, and orbiter contact points. Animations were developed using mechanisms to reflect each storyboard. Every effort was made to build all models technically correct for engineering use. The result was an animated routine that could be used by NASA for training material handlers and uncovering engineering safety issues.

ADDITIONAL STRESS AND FRACTURE MECHANICS ANALYSES OF PRESSURIZED WATER REACTOR PRESSURE VESSEL NOZZLES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walter, Matthew; Yin, Shengjun; Stevens, Gary

2012-01-01

In past years, the authors have undertaken various studies of nozzles in both boiling water reactors (BWRs) and pressurized water reactors (PWRs) located in the reactor pressure vessel (RPV) adjacent to the core beltline region. Those studies described stress and fracture mechanics analyses performed to assess various RPV nozzle geometries, which were selected based on their proximity to the core beltline region, i.e., those nozzle configurations that are located close enough to the core region such that they may receive sufficient fluence prior to end-of-life (EOL) to require evaluation of embrittlement as part of the RPV analyses associated with pressure-temperaturemore » (P-T) limits. In this paper, additional stress and fracture analyses are summarized that were performed for additional PWR nozzles with the following objectives: To expand the population of PWR nozzle configurations evaluated, which was limited in the previous work to just two nozzles (one inlet and one outlet nozzle). To model and understand differences in stress results obtained for an internal pressure load case using a two-dimensional (2-D) axi-symmetric finite element model (FEM) vs. a three-dimensional (3-D) FEM for these PWR nozzles. In particular, the ovalization (stress concentration) effect of two intersecting cylinders, which is typical of RPV nozzle configurations, was investigated. To investigate the applicability of previously recommended linear elastic fracture mechanics (LEFM) hand solutions for calculating the Mode I stress intensity factor for a postulated nozzle corner crack for pressure loading for these PWR nozzles. These analyses were performed to further expand earlier work completed to support potential revision and refinement of Title 10 to the U.S. Code of Federal Regulations (CFR), Part 50, Appendix G, Fracture Toughness Requirements, and are intended to supplement similar evaluation of nozzles presented at the 2008, 2009, and 2011 Pressure Vessels and Piping (PVP) Conferences. This work is also relevant to the ongoing efforts of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel (B&PV) Code, Section XI, Working Group on Operating Plant Criteria (WGOPC) efforts to incorporate nozzle fracture mechanics solutions into a revision to ASME B&PV Code, Section XI, Nonmandatory Appendix G.« less

ARC Software and Models

Science.gov Websites

produce software code and methodologies that are transferred to TARDEC and industry partners. These constraints", ASME Dynamic Systems and Control Conference, 2013, DOI:10.1115/DSCC2013-3935 Software Monitoring",IEEE Transactions on Control Systems Technology, DOI:10.1109/TCST.2012.2217143 Fast

FY16 ASME High Temperature Code Activities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Swindeman, M. J.; Jetter, R. I.; Sham, T. -L.

2016-09-01

One of the objectives of the ASME high temperature Code activities is to develop and validate both improvements and the basic features of Section III, Division 5, Subsection HB, Subpart B (HBB). The overall scope of this task is to develop a computer program to be used to assess whether or not a specific component under specified loading conditions will satisfy the elevated temperature design requirements for Class A components in Section III, Division 5, Subsection HB, Subpart B (HBB). There are many features and alternative paths of varying complexity in HBB. The initial focus of this task is amore » basic path through the various options for a single reference material, 316H stainless steel. However, the program will be structured for eventual incorporation all the features and permitted materials of HBB. Since this task has recently been initiated, this report focuses on the description of the initial path forward and an overall description of the approach to computer program development.« less

Creep-fatigue interaction at high temperature; Proceedings of the Symposium, 112th ASME Winter Annual Meeting, Atlanta, GA, Dec. 1-6, 1991

NASA Astrophysics Data System (ADS)

Haritos, George K.; Ochoa, O. O.

Various papers on creep-fatigue interaction at high temperature are presented. Individual topics addressed include: analysis of elevated temperature fatigue crack growth mechanisms in Alloy 718, physically based microcrack propagation laws for creep-fatigue-environment interaction, in situ SEM observation of short fatigue crack growth in Waspaloy at 700 C under cyclic and dwell conditions, evolution of creep-fatigue life prediction models, TMF design considerations in turbine airfoils of advanced turbine engines. Also discussed are: high temperature fatigue life prediction computer code based on the total strain version of strainrange partitioning, atomic theory of thermodynamics of internal variables, geometrically nonlinear analysis of interlaminar stresses in unsymmetrically laminated plates subjected to uniform thermal loading, experimental investigation of creep crack tip deformation using moire interferometry. (For individual items see A93-31336 to A93-31344)

Proceedings of the Third NRC/ASME Symposium on Valve and Pump Testing. Session 1A--Session 2C: Volume 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1994-07-01

The 1994 Symposium on Valve and Pump Testing, jointly sponsored by the Board of Nuclear Codes and Standards of the American Society of Mechanical Engineers and by the Nuclear Regulatory Commission, provides a forum for the discussion of current programs and methods for inservice testing and motor-operated valve testing at nuclear power plants. The symposium also provides an opportunity to discuss the need to improve that testing in order to help ensure the reliable performance of pumps and valves. The participation of industry representatives, regulators, and consultants results in the discussion of a broad spectrum of ideas and perspectives regardingmore » the improvement of inservice testing of pumps and valves at nuclear power plants. This document, Volume 1, covers sessions 1A through session 2C. The individual papers have been cataloged separately.« less

The Engaged Microbiologist: Bringing the Microbiological Sciences to the K–12 Community

PubMed Central

Westenberg, David J.

2016-01-01

Exposing K–12 students to cutting edge science that impacts their daily lives can bring classroom lessons to life. Citizen-science projects are an excellent way to bring high-level science to the classroom and help satisfy one of the cornerstone concepts of the Next Generation Science Standards (NGSS), “engaging in practices that scientists and engineers actually use.” This can be a daunting task for teachers who may lack the background or resources to integrate these projects into the classroom. This is where scientific societies such as the American Society for Microbiology (ASM) can play a critical role. ASM encourages its members to engage with the K–12 community by providing networking opportunities and resources for ASM members and K–12 teachers to work together to bring microbiology into the classroom. Journal of Microbiology & Biology Education PMID:27047585

Non Destructive Test Dye Penetrant and Ultrasonic on Welding SMAW Butt Joint with Acceptance Criteria ASME Standard

NASA Astrophysics Data System (ADS)

Endramawan, T.; Sifa, A.

2018-02-01

The purpose of this research is to know the type of discontinuity of SMAW welding result and to determine acceptance criteria based on American Society of Mechanical Engineer (ASME) standard. Material used is mild steel 98,71% Fe and 0,212% C with hardness 230 VHN with specimen diameter 20 cm and thickness 1.2 cm which is welded use SMAW butt joint with electrode for rooting LB 52U diameter 2.6 mm, current 70 Ampere and voltage 380 volt, filler used LB 5218 electrode diameter 3.2 mm with current 80 Ampere and 380 volt. The method used to analyze the welded with non destructive test dye penetrant (PT) method to see indication on the surface of the object and Ultrasonic (UT) to see indication on the sub and inner the surface of the object, the result is discontinuity recorded and analyzed and then the discontinuity is determine acceptance criteria based on the American Society of Mechanical Engineer (ASME) standards. The result show the discontinuity of porosity on the surface of the welded and inclusion on sub material used ultrasonic test, all indication on dye penetrant or ultrasonic test if there were rejected of result of welded that there must be gouging on part which rejected and then re-welding.

49 CFR 178.345-14 - Marking.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Specifications for Containers for Motor Vehicle Transportation § 178.345-14 Marking. (a) General. The manufacturer shall certify that each cargo tank motor vehicle has been designed, constructed and tested in....348) cargo tank requirements and, when applicable, with Section VIII of the ASME Code (IBR, see § 171...

Multidisciplinary Computational Research

DTIC Science & Technology

2006-07-01

Aeronautical Society Silver Award and Busk Prize (2006);USAF Basic Research Award (2004); AFRL Fellow (1995); Outstanding Scientist of Dayton (2003...Speaker, ASME 2006 Fluids Engineering Conference; Royal Aeronautical Society Silver Award and Busk Prize (2006). Rizzetta, D., Gen. B. Foulois

MIDWEST STRUCTURAL SCIENCES CENTER 2011 ANNUAL REPORT

DTIC Science & Technology

2011-10-01

S. MICHAEL SPOTTSWOOD MICHAEL J. SHEPARD , Chief Senior Aerospace Engineer Analytical Mechanics Branch Analytical...49th AIAA/ASME/ASCE/AHS/ASC Structures, Structural Dynamics & Materials Confe- rence, Chicago , IL, Apr. 7-10, 2008. AIAA 2008-2077. Efstathiou C

ARC Researchers at ASME 2015 Internal Combustion Engine Division Fall

Science.gov Websites

-sense. Therefore, the focus of this paper is on the various methods of computing CA50 for analysing and classifying cycle-to-cycle variability. The assumptions made to establish fast and possibly on-line methods SI engine. Then the various fast methods for computing CA50 feed the two statistical methods

Light Weight Portable Plasma Medical Device - Plasma Engineering Research Laboratory

DTIC Science & Technology

2011-10-01

Millennial Student. 15. Thiyagarajan, M. (2011). Portable Plasma Biomedical Device for Cancer Treatment. Irvine, California: ASME Emerging...American Society of Mechanical Engineers Sigma Xi Toastmasters International Club MIT Entrepreneur Club Eta Kappa Nu Tau Beta Pi Institute of...Learning Environment. Corpus Christi, TX: TAMUCC 1st Faculty Symposium: Course Design for the Millennial Student. Thiyagarajan, M. (2011). Portable

Nonlinear Analysis of Mechanical Systems Under Combined Harmonic and Stochastic Excitation

DTIC Science & Technology

1993-05-27

Namachchivaya and Naresh Malhotra Department of Aeronautical and Astronautical Engineering University of Illinois, Urbana-Champaign Urbana, Illinois...Aeronauticai and Astronautical Engineering, University of Illinois, 1991. 2. N. Sri Namachchivaya and N. Malhotra , Parametrically Excited Hopf Bifurcation...Namachchivaya and N. Malhotra , Parametrically Excited Hopf Bifurcation with Non-semisimple 1:1 Resonance, Nonlinear Vibrations, ASME-AMD, Vol. 114, 1992. 3

49 CFR 178.337-3 - Structural integrity.

Code of Federal Regulations, 2010 CFR

2010-10-01

... calculated design stress at any point in the cargo tank may not exceed the maximum allowable stress value... maximum design stress at any point in the cargo tank must be calculated separately for the loading... with Section VIII of the ASME Code. The cargo tank design must include calculation of stresses...

46 CFR 64.5 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., excluding the allowance for corrosion and the thickness for loadings other than pressure, as provided for in the ASME Code. (e) Test pressure means a hydrostatic pressure of at least one and one-half times the maximum allowable working pressure. (f) Dynamic loading conditions means the following: (1) A loading in...

46 CFR 64.5 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., excluding the allowance for corrosion and the thickness for loadings other than pressure, as provided for in the ASME Code. (e) Test pressure means a hydrostatic pressure of at least one and one-half times the maximum allowable working pressure. (f) Dynamic loading conditions means the following: (1) A loading in...

46 CFR 64.5 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., excluding the allowance for corrosion and the thickness for loadings other than pressure, as provided for in the ASME Code. (e) Test pressure means a hydrostatic pressure of at least one and one-half times the maximum allowable working pressure. (f) Dynamic loading conditions means the following: (1) A loading in...

46 CFR 64.5 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., excluding the allowance for corrosion and the thickness for loadings other than pressure, as provided for in the ASME Code. (e) Test pressure means a hydrostatic pressure of at least one and one-half times the maximum allowable working pressure. (f) Dynamic loading conditions means the following: (1) A loading in...

49 CFR 178.345-10 - Pressure relief.

Code of Federal Regulations, 2010 CFR

2010-10-01

... applicable individual specification. The pressure and vacuum relief system must be designed to operate and... resulting from loading, unloading, or from heating and cooling of lading. Pressure relief systems are not required to conform to the ASME Code. (b) Type and construction of relief systems and devices. (1) Each...

FY 2017-Progress Report on the Design and Construction of the Sodium Loop SMT-3

DOE Office of Scientific and Technical Information (OSTI.GOV)

Natesan, K.; Momozaki, Y.

This report provides an update on the design of a forced-convection sodium loop to be used for the evaluation of sodium compatibility of advanced Alloy 709 with emphasis on long term exposures of tensile, creep, fatigue, creep fatigue, and fracture toughness ASTM-size specimens in support of ASME Code qualification and NRC licensing. The report is a deliverable (Level 4) in FY17 (M4AT-17AN1602094), under the Work Package AT-17AN160209, “Sodium Compatibility” performed by Argonne National Laboratory (ANL), as part of the Advanced Materials Program for the Advanced Reactor Technology. This work package enables the development of advanced structural materials by providing corrosion,more » microstructure, and mechanical property data from the standpoint of sodium compatibility of advanced structural alloys. The first sodium loop (SMT-1) with a single tank was constructed in 2011 at ANL and has been in operation for exposure of subsize sheet specimens of advanced alloys at a single temperature. The second sodium loop with dual tanks (SMT-2) was constructed in 2013 and has been in operation for the exposure of subsize sheet specimens of advanced alloys at two different temperatures. The current loop (SMT-3) has been designed to incorporate sufficient chamber capacity to expose a large number of ASTM-size specimens to evaluate the sodium effects on tensile, creep, fatigue, creep-fatigue, and fracture toughness properties, in support of ASME Code Qualification and USNRC Licensing. The design of individual components for the third sodium loop SMT-3 is almost complete. The design also has been sent to an outside vendor for piping analysis to be in compliance with ASME Code. A purchase order has been placed with an outside vendor for the fabrication of major components such as the specimen exposure tanks. However, we have contracted with another vendor to establish the piping design in compliance with ASME design codes. The piping design was completed in FY2017 and the information is being transmitted to the tank fabricator. The SMT-3 loop will be located in Building 206 adjacent to the currently operating SMT-2 loop. In addition, we have demolished the aged power supply system in Building 206 and installed a new transformer, wiring, and power panels for the new loop. Procurement of some of the long lead items such as valves, EM pumps, EM flowmeters, etc. is in progress and will continue in FY 2018. The construction of components such as cold trap, economizers, piping arrangement etc. will be performed in the central shops at ANL. About 150 liters of sodium for the loop will be procured in early FY2018. The loop system is designed to circulate sodium through the sample tanks and the associated loop without an operator for an extended period of time. With the three sodium loops (with single-tank, dual-tank and four–tanks), materials can be tested at different sodium temperatures, and large tensile, creep, fatigue, creep-fatigue, and fracture toughness specimens can be exposed to sodium for extended periods of time and generate data on mechanical properties in support of ASME Code Qualification and USNRC Licensing of advanced Alloy 709 for use as a structural material in SFRs.« less

Ratchetting in pressurized pipes

NASA Astrophysics Data System (ADS)

Rider, R. J.; Harvey, S. J.; Charles, I. D.

1994-04-01

The plastic deformation of thin-walled cylinders has been experimentally examined for the loading conditions of +/- 1% axial strain with hoop stresses of approximately 0, 1/4, 1/2 and 3/4 of the initial uniaxial yield stress. Two materials similar to those used in the pipework of PWR nuclear plant in the U.K. have been tested, namely 304S11 stainless steel and En6 low-carbon steel. The results of the tests were to be compared with the allowable stresses and deformations specified in the ASME Boiler and Pressure Vessel Code, Section III. The code specifies that a prescribed combination of primary stresses must not exceed 1.5S(sub m), where S(sub m) is a stress value defined for each material. The results indicate that the limit of 1.5S(sub m) is excessively low for both materials and that in particular, the stainless steel could tolerate 5S(sub m). Although the En6 steel is more prone to ratchetting than the stainless steel, the results suggest that it too could tolerate a higher primary stress than the code allows. Both materials are shown to satisfy the proposed ASME ratchet strain limit of 5% hoop strain after 10 cycles of +/- 1% axial strain range, for any value of internal pressure.

Advancing Aeronautics: A Decision Framework for Selecting Research Agendas

DTIC Science & Technology

2011-01-01

Engineering Board ASME American Society of Mechanical Engineers ATA Air Transport Association ATM air traffic management ATP Aeronautics Test Program...not provide a competitive advantage for a sufficient period to justify the R&D outlay. Such projects can include technolo- gies that address...fuel efficiency improvements multiplied by the cost of fuel would provide an estimate of their value to society. Likewise, technolo- gies for

Status of nuclear Class 1 component requalification: Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cooper, W.E.

1986-12-01

Qualification relates to assurance of acceptability of the component with respect to structural integrity, operability and functional capability. Requalification is required if existing qualification is lost because of: expiration of the qualified service life (life extension); reactivation of a cancelled or suspended plant; failure to conform with certain requirements of the Technical Specifications, or revision to the applicable Regulatory requirements. The alternatives to requalification are replacement or removal from service. The choice between requalification, replacement and removal from service is governed by economics. The purpose of requalification standards is to ensure the acceptability of the requalification process. A previous EPRImore » Report prepared by Teledyne Engineering Services (TES) (NP-1921) developed a rationale for, and a draft of, a generic requalification standard for Class 1 Pressure Boundary Components presently considered by the Boiler and Pressure Vessel Code published by The American Society of Mechanical Engineers (ASME/BPVC). International Energy Associates Limited (IEA) prepared another report for EPRI shortly thereafter (NP-2418), which reviewed the economic and technologies factors of nuclear plant life extension, and concluded that NP-1921 makes a strong case that the nuclear industry will benefit from the development of the proposed standard.« less

Fabrication development for high-level nuclear waste containers for the tuff repository; Phase 1 final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Domian, H.A.; Holbrook, R.L.; LaCount, D.F.

1990-09-01

This final report completes Phase 1 of an engineering study of potential manufacturing processes for the fabrication of containers for the long-term storage of nuclear waste. An extensive literature and industry review was conducted to identify and characterize various processes. A technical specification was prepared using the American Society of Mechanical Engineers Boiler & Pressure Vessel Code (ASME BPVC) to develop the requirements. A complex weighting and evaluation system was devised as a preliminary method to assess the processes. The system takes into account the likelihood and severity of each possible failure mechanism in service and the effects of variousmore » processes on the microstructural features. It is concluded that an integral, seamless lower unit of the container made by back extrusion has potential performance advantages but is also very high in cost. A welded construction offers lower cost and may be adequate for the application. Recommendations are made for the processes to be further evaluated in the next phase when mock-up trials will be conducted to address key concerns with various processes and materials before selecting a primary manufacturing process. 43 refs., 26 figs., 34 tabs.« less

10 CFR 851.27 - Reference sources.

Code of Federal Regulations, 2011 CFR

2011-01-01

....nfpa.org. (v) American Conference of Governmental Industrial Hygienist (ACGIH), 1330 Kemper Meadow...) American Society of Mechanical Engineers (ASME), P.O. Box 2300 Fairfield, NJ 07007. Telephone: 800-843-2763...) American Conference of Governmental Industrial Hygienists, “Threshold Limit Values for Chemical Substances...

U.S. Naval Research Laboratory Final Analysis Report to NATO Above Water Warfare Capabilities Group 2016 Naval Electromagnetic Operations Trials

DTIC Science & Technology

2017-05-23

Systems and the NRL Code 5763 Radio Frequency (RF) Stimulator. It includes and covers system descriptions , setup, data collection, and test goals that...6 4. Test Asset Descriptions ...7 4.1. Description of FOXTROT Anti-ship Missile (ASM) Simulator ......................................... 7

49 CFR 179.400-3 - Type.

Code of Federal Regulations, 2010 CFR

2010-10-01

... essentially concentric within an outer jacket of circular cross section, with the out of roundness of both the inner tank and outer jacket limited in accordance with Paragraph UG-80 in Section VIII of the ASME Code (IBR, see § 171.7 of this subchapter); (2) Have the annular space evacuated after filling the annular...

49 CFR 179.400-3 - Type.

Code of Federal Regulations, 2011 CFR

2011-10-01

... essentially concentric within an outer jacket of circular cross section, with the out of roundness of both the inner tank and outer jacket limited in accordance with Paragraph UG-80 in Section VIII of the ASME Code (IBR, see § 171.7 of this subchapter); (2) Have the annular space evacuated after filling the annular...

49 CFR 179.400-3 - Type.

Code of Federal Regulations, 2012 CFR

2012-10-01

... essentially concentric within an outer jacket of circular cross section, with the out of roundness of both the inner tank and outer jacket limited in accordance with Paragraph UG-80 in Section VIII of the ASME Code (IBR, see § 171.7 of this subchapter); (2) Have the annular space evacuated after filling the annular...

49 CFR 179.400-3 - Type.

Code of Federal Regulations, 2014 CFR

2014-10-01

... essentially concentric within an outer jacket of circular cross section, with the out of roundness of both the inner tank and outer jacket limited in accordance with Paragraph UG-80 in Section VIII of the ASME Code (IBR, see § 171.7 of this subchapter); (2) Have the annular space evacuated after filling the annular...

46 CFR 64.13 - Allowable stress; tank.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Allowable stress; tank. 64.13 Section 64.13 Shipping... CARGO HANDLING SYSTEMS Standards for an MPT § 64.13 Allowable stress; tank. (a) The calculated stress in... not exceed the allowable stress listed in Division 1 of section VIII of the ASME Code, for a design...

49 CFR 195.3 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Fittings Industry, Inc. (MSS), 127 Park Street, NE., Vienna, VA 22180. 5. American Society for Testing and...)) §§ 195.205(b)(1); 195.432(b). (12) API Standard 1104, “Welding of Pipelines and Related Facilities” (20th....307(e). (7) 2007 ASME Boiler & Pressure Vessel Code, Section IX: “Qualification Standard for Welding...

49 CFR 178.338-16 - Inspection and testing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Inspection and testing. 178.338-16 Section 178.338... PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.338-16 Inspection and testing... Section VIII of the ASME Code. The welder and the welding procedure must be qualified in accordance with...

46 CFR 56.70-5 - Material.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Material. 56.70-5 Section 56.70-5 Shipping COAST GUARD..., Assembly and Erection § 56.70-5 Material. (a) Filler metal. All filler metal, including consumable insert material, must comply with the requirements of section IX of the ASME Boiler and Pressure Vessel Code...

46 CFR 56.70-5 - Material.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Material. 56.70-5 Section 56.70-5 Shipping COAST GUARD..., Assembly and Erection § 56.70-5 Material. (a) Filler metal. All filler metal, including consumable insert material, must comply with the requirements of section IX of the ASME Boiler and Pressure Vessel Code...

46 CFR 64.13 - Allowable stress; tank.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Allowable stress; tank. 64.13 Section 64.13 Shipping... CARGO HANDLING SYSTEMS Standards for an MPT § 64.13 Allowable stress; tank. (a) The calculated stress in... not exceed the allowable stress listed in Division 1 of section VIII of the ASME Code, for a design...

46 CFR 64.13 - Allowable stress; tank.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Allowable stress; tank. 64.13 Section 64.13 Shipping... CARGO HANDLING SYSTEMS Standards for an MPT § 64.13 Allowable stress; tank. (a) The calculated stress in... not exceed the allowable stress listed in Division 1 of section VIII of the ASME Code, for a design...

46 CFR 64.13 - Allowable stress; tank.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Allowable stress; tank. 64.13 Section 64.13 Shipping... CARGO HANDLING SYSTEMS Standards for an MPT § 64.13 Allowable stress; tank. (a) The calculated stress in... not exceed the allowable stress listed in Division 1 of section VIII of the ASME Code, for a design...

USSR Report, Cybernetics, Computers and Automation Technology

DTIC Science & Technology

1987-03-31

version of the system was tested by adapting PAL-11 and MACRO-11 assembly code for the "Elektronika=60" and "Elektronika-60M" computers; ASM -86 for the...GS, "On the Results of Evaluation of Insurance Payments in Collective and State Farms and Private Households," the actuarial analysis tables based

46 CFR 64.13 - Allowable stress; tank.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Allowable stress; tank. 64.13 Section 64.13 Shipping... CARGO HANDLING SYSTEMS Standards for an MPT § 64.13 Allowable stress; tank. (a) The calculated stress in... not exceed the allowable stress listed in Division 1 of section VIII of the ASME Code, for a design...

78 FR 48727 - Proposed Revisions to Design of Structures, Components, Equipment and Systems

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-09

... Analysis Reports for Nuclear Power Plants: LWR Edition,'' Section 3.9.3 ``ASME Code Class 1, 2, and 3...'s Agencywide Documents Access and Management System (ADAMS): You may access publicly available... operational readiness of snubbers (ADAMS Accession No. ML070720041), and review interfaces have been updated...

Structural integrity of a confinement vessel for testing nuclear fuels for space propulsion

NASA Astrophysics Data System (ADS)

Bergmann, V. L.

Nuclear propulsion systems for rockets could significantly reduce the travel time to distant destinations in space. However, long before such a concept can become reality, a significant effort must be invested in analysis and ground testing to guide the development of nuclear fuels. Any testing in support of development of nuclear fuels for space propulsion must be safely contained to prevent the release of radioactive materials. This paper describes analyses performed to assess the structural integrity of a test confinement vessel. The confinement structure, a stainless steel pressure vessel with bolted flanges, was designed for operating static pressures in accordance with the ASME Boiler and Pressure Vessel Code. In addition to the static operating pressures, the confinement barrier must withstand static overpressures from off-normal conditions without releasing radioactive material. Results from axisymmetric finite element analyses are used to evaluate the response of the confinement structure under design and accident conditions. For the static design conditions, the stresses computed from the ASME code are compared with the stresses computed by the finite element method.

Role of non-coding RNAs in maintaining primary airway smooth muscle cells

PubMed Central

2014-01-01

Background The airway smooth muscle (ASM) cell maintains its own proliferative rate and contributes to the inflammatory response in the airways, effects that are inhibited by corticosteroids, used in the treatment of airways diseases. Objective We determined the differential expression of mRNAs, microRNAs (miRNAs) and long noncoding RNA species (lncRNAs) in primary ASM cells following treatment with a corticosteroid, dexamethasone, and fetal calf serum (FCS). Methods mRNA, miRNA and lncRNA expression was measured by microarray and quantitative real-time PCR. Results A small number of miRNAs (including miR-150, −371-5p, −718, −940, −1181, −1207-5p, −1915, and −3663-3p) were decreased following exposure to dexamethasone and FCS. The mRNA targets of these miRNAs were increased in expression. The changes in mRNA expression were associated with regulation of ASM actin cytoskeleton. We also observed changes in expression of lncRNAs, including natural antisense, pseudogenes, intronic lncRNAs, and intergenic lncRNAs following dexamethasone and FCS. We confirmed the change in expression of three of these, LINC00882, LINC00883, PVT1, and its transcriptional activator, c-MYC. We propose that four of these lincRNAs (RP11-46A10.4, LINC00883, BCYRN1, and LINC00882) act as miRNA ‘sponges’ for 4 miRNAs (miR-150, −371-5p, −940, −1207-5p). Conclusion This in-vitro model of primary ASM cell phenotype was associated with the regulation of several ncRNAs. Their identification allows for in-vitro functional experimentation to establish causality with the primary ASM phenotype, and in airway diseases such as asthma and chronic obstructive pulmonary disease (COPD). PMID:24886442

Heidi Pilath | NREL

Science.gov Websites

; Journal of Solar Energy Engineering-Transactions of the Asme 128(1): 54-57. Clark, J., et al. (2015 inorganic anions by ion chromatography with suppressed conductivity detection." Journal of vapors produced from Nordic feedstocks in cyclone pyrolysis." Journal of Analytical and Applied

Autoignition and Burning Speeds of JP-8 Fuel at High Temperatures and Pressures

DTIC Science & Technology

2004-08-25

Editorial Board of the International Journal of Exergy . He is also a member of the Scientific Council of International Center for Applied Thermodynamics...for Schlieren and Shadowgraph Images of Transient Expanding Spherical Thin Flames, ASME International Journal of Engineering for Gas Turbines and...Measurements of Methane-Oxygen-Argon Mixtures and Its Application to Extend the Methane-Air Burning Velocity Measurements”, International Journal of Engine

Comprehensive Report For Proposed Elevated Temperature Elastic Perfectly Plastic (EPP) Code Cases Representative Example Problems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hollinger, Greg L.

Background: The current rules in the nuclear section of the ASME Boiler and Pressure Vessel (B&PV) Code , Section III, Subsection NH for the evaluation of strain limits and creep-fatigue damage using simplified methods based on elastic analysis have been deemed inappropriate for Alloy 617 at temperatures above 1200F (650C)1. To address this issue, proposed code rules have been developed which are based on the use of elastic-perfectly plastic (E-PP) analysis methods and which are expected to be applicable to very high temperatures. The proposed rules for strain limits and creep-fatigue evaluation were initially documented in the technical literature 2,more » 3, and have been recently revised to incorporate comments and simplify their application. The revised code cases have been developed. Task Objectives: The goal of the Sample Problem task is to exercise these code cases through example problems to demonstrate their feasibility and, also, to identify potential corrections and improvements should problems be encountered. This will provide input to the development of technical background documents for consideration by the applicable B&PV committees considering these code cases for approval. This task has been performed by Hollinger and Pease of Becht Engineering Co., Inc., Nuclear Services Division and a report detailing the results of the E-PP analyses conducted on example problems per the procedures of the E-PP strain limits and creep-fatigue draft code cases is enclosed as Enclosure 1. Conclusions: The feasibility of the application of the E-PP code cases has been demonstrated through example problems that consist of realistic geometry (a nozzle attached to a semi-hemispheric shell with a circumferential weld) and load (pressure; pipe reaction load applied at the end of the nozzle, including axial and shear forces, bending and torsional moments; through-wall transient temperature gradient) and design and operating conditions (Levels A, B and C).« less

16 CFR 1450.2 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Definitions. 1450.2 Section 1450.2... GRAEME BAKER POOL AND SPA SAFETY ACT REGULATIONS § 1450.2 Definitions. (a) [Reserved] (b) Unblockable... American Society of Mechanical Engineers (ASME), ATTN: Secretary, A112 Standards Committee, Three Park...

Vehicle Exhaust Waste Heat Recovery Model with Integrated Thermal Load Leveling

DTIC Science & Technology

2015-08-01

recovery from automobile engine. Energy. 2010;35:1447–1454. 14 12. Ceraianu MO, Gontean A. Parasitic elements modelling in thermoelectric...JP, Sampath S. Heat transfer modeling and geometry optimization of TEG for automobile applications. Proceedings of the ASME Summer Heat Transfer

46 CFR 151.01-2 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., are: American Society for Nondestructive Testing (ASNT) 4153 Arlingate Road, Caller #28518, Columbus... Certification in Nondestructive Testing” 151.04-7(c)(2) American Society of Mechanical Engineers (ASME..., Nondestructive Examination (1986) 151.04-7(a)(1) American Society for Testing and Materials (ASTM) 100 Barr...

Tribological Limitations in Gas Turbine Engines: A Workshop to Identify the Challenges and Set Future Directions

NASA Technical Reports Server (NTRS)

DellaCorte, Chris; Pinkus, Oscar

2000-01-01

The following report represents a compendium of selected speaker presentation materials and observations made by Prof O. Pinkus at the NASA/ASME/Industry sponsored workshop entitled "Tribological Limitations in Gas Turbine Engines" held on September 15-17, 1999 in Albany, New York. The impetus for the workshop came from the ASME's Research Committee on Tribology whose goal is to explore new tribological research topics which may become future research opportunities. Since this subject is of current interest to other industrial and government entities the conference received cosponsorship as noted above. The conference was well attended by government, industrial and academic participants. Topics discussed included current tribological issues in gas turbines as well as the potential impact (drawbacks and advantages) of future tribological technologies especially foil air bearings and magnetic beatings. It is hoped that this workshop report may serve as a starting point for continued discussions and activities in oil-free turbomachinery systems.

Tribological Limitations in Gas Turbine Engines: A Workshop to Identify the Challenges and Set Future Directions. Revised

NASA Technical Reports Server (NTRS)

DellaCorte, Chris; Pinkus, Oscar

2002-01-01

The following report represents a compendium of selected speaker presentation materials and observations made by Prof. O. Pinkus at the NASA/ASME/Industry sponsored workshop entitled "Tribological Limitations in Gas Turbine Engines" held on September 15-17, 1999 in Albany, New York. The impetus for the workshop came from the ASME's Research Committee on tribology whose goal is to explore new tribological research topics which may become future research opportunities. Since this subject is of current interest to other industrial and government entities the conference received cosponsorship as noted above. The conference was well attended by government, industrial, and academic participants. Topics discussed included current tribological issues in gas turbines as well as the potential impact (drawbacks and advantages) of future tribological technologies especially foil air bearings and magnetic bearings. It is hoped that this workshop report may serve as a starting point for continued discussions and activities in oil-free turbomachinery systems.

Calibration of the NASA GRC 16 In. Mass-Flow Plug

NASA Technical Reports Server (NTRS)

Davis, David O.; Friedlander, David J.; Saunders, J. David; Frate, Franco C.; Foster, Lancert E.

2012-01-01

The results of an experimental calibration of the NASA Glenn Research Center 16 in. Mass-Flow Plug (MFP) are presented and compared to a previously obtained calibration of a 15 in. Mass-Flow Plug. An ASME low-beta, long-radius nozzle was used as the calibration reference. The discharge coefficient for the ASME nozzle was obtained by numerically simulating the flow through the nozzle from the WIND-US code. The results showed agreement between the 15 in. and 16 in. MFPs for area ratios (MFP to pipe area ratio) greater than 0.6 but deviate at area ratios below this value for reasons that are not fully understood. A general uncertainty analysis was also performed and indicates that large uncertainties in the calibration are present for low MFP area ratios.

Calibration of the NASA Glenn Research Center 16 in. Mass-Flow Plug

NASA Technical Reports Server (NTRS)

Davis, David O.; Friedlander, David J.; Saunders, J. David; Frate, Franco C.; Foster, Lancert E.

2014-01-01

The results of an experimental calibration of the NASA Glenn Research Center 16 in. Mass-Flow Plug (MFP) are presented and compared to a previously obtained calibration of a 15 in. Mass-Flow Plug. An ASME low-beta, long-radius nozzle was used as the calibration reference. The discharge coefficient for the ASME nozzle was obtained by numerically simulating the flow through the nozzle from the WIND-US code. The results showed agreement between the 15 and 16 in. MFPs for area ratios (MFP to pipe area ratio) greater than 0.6 but deviate at area ratios below this value for reasons that are not fully understood. A general uncertainty analysis was also performed and indicates that large uncertainties in the calibration are present for low MFP area ratios.

Weld Repair of a Stamped Pressure Vessel in a Radiologically Controlled Zone

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cannell, Gary L.; Huth, Ralph J.; Hallum, Randall T.

2013-08-26

In September 2012 an ASME B&PVC Section VIII stamped pressure vessel located at the DOE Hanford Site Effluent Treatment Facility (ETF) developed a through-wall leak. The vessel, a steam/brine heat exchanger, operated in a radiologically controlled zone (by the CH2MHill PRC or CHPRC), had been in service for approximately 17 years. The heat exchanger is part of a single train evaporator process and its failure caused the entire system to be shut down, significantly impacting facility operations. This paper describes the activities associated with failure characterization, technical decision making/planning for repair by welding, logistical challenges associated with performing work inmore » a radiologically controlled zone, performing the repair, and administrative considerations related to ASME code requirements.« less

49 CFR 178.277 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2011 CFR

2011-10-01

... design pressure in the ASME Code, Section VIII (IBR, see § 171.7 of this subchapter). Holding time is the... 49 Transportation 3 2011-10-01 2011-10-01 false Requirements for the design, construction... FOR PACKAGINGS Specifications for Portable Tanks § 178.277 Requirements for the design, construction...

49 CFR 178.277 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2012 CFR

2012-10-01

... design pressure in the ASME Code, Section VIII (IBR, see § 171.7 of this subchapter). Holding time is the... 49 Transportation 3 2012-10-01 2012-10-01 false Requirements for the design, construction... FOR PACKAGINGS Specifications for Portable Tanks § 178.277 Requirements for the design, construction...

49 CFR 178.277 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2014 CFR

2014-10-01

... design pressure in the ASME Code, Section VIII (IBR, see § 171.7 of this subchapter). Holding time is the... 49 Transportation 3 2014-10-01 2014-10-01 false Requirements for the design, construction... FOR PACKAGINGS Specifications for Portable Tanks § 178.277 Requirements for the design, construction...

49 CFR 178.277 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2010 CFR

2010-10-01

... design pressure in the ASME Code, Section VIII (IBR, see § 171.7 of this subchapter). Holding time is the... 49 Transportation 2 2010-10-01 2010-10-01 false Requirements for the design, construction... FOR PACKAGINGS Specifications for Portable Tanks § 178.277 Requirements for the design, construction...

49 CFR 178.277 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2013 CFR

2013-10-01

... design pressure in the ASME Code, Section VIII (IBR, see § 171.7 of this subchapter). Holding time is the... 49 Transportation 3 2013-10-01 2013-10-01 false Requirements for the design, construction... FOR PACKAGINGS Specifications for Portable Tanks § 178.277 Requirements for the design, construction...

77 FR 27428 - Certain Large Diameter Carbon and Alloy Seamless Standard, Line, and Pressure Pipe (Over 41/2

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-10

... diameter seamless pipe is used primarily for line applications such as oil, gas, or water pipeline, or utility distribution systems. Seamless pressure pipes are intended for the conveyance of water, steam... (``ASME'') code stress levels. Alloy pipes made to ASTM A-335 standard must be used if temperatures and...

76 FR 66688 - Certain Large Diameter Carbon and Alloy Seamless Standard, Line, and Pressure Pipe (Over 41/2

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-27

... diameter seamless pipe is used primarily for line applications such as oil, gas, or water pipeline, or utility distribution systems. Seamless pressure pipes are intended for the conveyance of water, steam... (``ASME'') code stress levels. Alloy pipes made to ASTM A-335 standard must be used if temperatures and...

Gas tungsten arc welding of aluminum alloys 6XXX. Welding procedure specification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wodtke, C.H.; Frizzell, D.R.; Plunkett, W.A.

1985-08-01

Procedure WPS-1003 is qualified under Section IX of the ASME Boiler and Pressure Vessel Code for gas tungsten arc welding of aluminum alloys 6061 and 6063 (P-23), in thickness range 0.035 to 0.516 in.; filler metal is ER4043 (F-23) or ER5356 (F-22); shielding gas is argon.

PWSCC Assessment by Using Extended Finite Element Method

NASA Astrophysics Data System (ADS)

Lee, Sung-Jun; Lee, Sang-Hwan; Chang, Yoon-Suk

2015-12-01

The head penetration nozzle of control rod driving mechanism (CRDM) is known to be susceptible to primary water stress corrosion cracking (PWSCC) due to the welding-induced residual stress. Especially, the J-groove dissimilar metal weld regions have received many attentions in the previous studies. However, even though several advanced techniques such as weight function and finite element alternating methods have been introduced to predict the occurrence of PWSCC, there are still difficulties in respect of applicability and efficiency. In this study, the extended finite element method (XFEM), which allows convenient crack element modeling by enriching degree of freedom (DOF) with special displacement function, was employed to evaluate structural integrity of the CRDM head penetration nozzle. The resulting stress intensity factors of surface cracks were verified for the reliability of proposed method through the comparison with those suggested in the American Society of Mechanical Engineering (ASME) code. The detailed results from the FE analyses are fully discussed in the manuscript.

Design and Evaluation of a Chamber Capable of Containing the Detonation Effects of 40 Pounds of TNT

DTIC Science & Technology

1975-11-01

Boiler and Pressure Vessel Code , 197A. For the girth welds, short lengths of 26-in.-dia. pipe were tacked to the vessel around the...and tensile tests meet the requirements of Section IX, ASHE Boiler and Pressure Vessel Code , 1974. Weld made by W. H. Stefanov ■.^^ w-^iiMaLwärt...Macro Results Kos, No Remarl.s The results of the bond and it^nsile tests met the requirements of Section IX, ASME Boiler and Pressure

Code qualification of structural materials for AFCI advanced recycling reactors.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Natesan, K.; Li, M.; Majumdar, S.

2012-05-31

This report summarizes the further findings from the assessments of current status and future needs in code qualification and licensing of reference structural materials and new advanced alloys for advanced recycling reactors (ARRs) in support of Advanced Fuel Cycle Initiative (AFCI). The work is a combined effort between Argonne National Laboratory (ANL) and Oak Ridge National Laboratory (ORNL) with ANL as the technical lead, as part of Advanced Structural Materials Program for AFCI Reactor Campaign. The report is the second deliverable in FY08 (M505011401) under the work package 'Advanced Materials Code Qualification'. The overall objective of the Advanced Materials Codemore » Qualification project is to evaluate key requirements for the ASME Code qualification and the Nuclear Regulatory Commission (NRC) approval of structural materials in support of the design and licensing of the ARR. Advanced materials are a critical element in the development of sodium reactor technologies. Enhanced materials performance not only improves safety margins and provides design flexibility, but also is essential for the economics of future advanced sodium reactors. Code qualification and licensing of advanced materials are prominent needs for developing and implementing advanced sodium reactor technologies. Nuclear structural component design in the U.S. must comply with the ASME Boiler and Pressure Vessel Code Section III (Rules for Construction of Nuclear Facility Components) and the NRC grants the operational license. As the ARR will operate at higher temperatures than the current light water reactors (LWRs), the design of elevated-temperature components must comply with ASME Subsection NH (Class 1 Components in Elevated Temperature Service). However, the NRC has not approved the use of Subsection NH for reactor components, and this puts additional burdens on materials qualification of the ARR. In the past licensing review for the Clinch River Breeder Reactor Project (CRBRP) and the Power Reactor Innovative Small Module (PRISM), the NRC/Advisory Committee on Reactor Safeguards (ACRS) raised numerous safety-related issues regarding elevated-temperature structural integrity criteria. Most of these issues remained unresolved today. These critical licensing reviews provide a basis for the evaluation of underlying technical issues for future advanced sodium-cooled reactors. Major materials performance issues and high temperature design methodology issues pertinent to the ARR are addressed in the report. The report is organized as follows: the ARR reference design concepts proposed by the Argonne National Laboratory and four industrial consortia were reviewed first, followed by a summary of the major code qualification and licensing issues for the ARR structural materials. The available database is presented for the ASME Code-qualified structural alloys (e.g. 304, 316 stainless steels, 2.25Cr-1Mo, and mod.9Cr-1Mo), including physical properties, tensile properties, impact properties and fracture toughness, creep, fatigue, creep-fatigue interaction, microstructural stability during long-term thermal aging, material degradation in sodium environments and effects of neutron irradiation for both base metals and weld metals. An assessment of modified versions of Type 316 SS, i.e. Type 316LN and its Japanese version, 316FR, was conducted to provide a perspective for codification of 316LN or 316FR in Subsection NH. Current status and data availability of four new advanced alloys, i.e. NF616, NF616+TMT, NF709, and HT-UPS, are also addressed to identify the R&D needs for their code qualification for ARR applications. For both conventional and new alloys, issues related to high temperature design methodology are described to address the needs for improvements for the ARR design and licensing. Assessments have shown that there are significant data gaps for the full qualification and licensing of the ARR structural materials. Development and evaluation of structural materials require a variety of experimental facilities that have been seriously degraded in the past. The availability and additional needs for the key experimental facilities are summarized at the end of the report. Detailed information covered in each Chapter is given.« less

Finite Strain Behavior of Polyurea for a Wide Range of Strain Rates

DTIC Science & Technology

2010-02-01

dimensional dynamic compressive behavior of EPDM rubber ," Journal of Engineering Materials and Technology, Transaction of the ASME, 125:294-301. [97] Song, B...and Chen, W. (2004) "Dynamic compressive behavior of EPDM rubber un- der nearly uniaxial strain conditions," Journal of Engineering Materials and... rubber elastic springs to describe the steep initial stiffness of virgin butadiene rubber under tensile and compressive loading at intermediate strain

FASTSim Publications | Transportation Research | NREL

Science.gov Websites

Applications Authors: K. Genikomsakis and G. Mitrentsis Journal: Transportation Research Part D: Transport and Vehicles for Better Greenhouse Gas Reduction Authors: K. Hamza and K. Laberteaux Conference: ASME Design Engineering Technical Conference (August 2016) Optimal Design and Techno-Economic Analysis of a Hybrid Solar

46 CFR 53.01-1 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

.... (b) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY...., 333 Pfingston Road, Northbrook, IL 60062-2096: (1) UL 174, Standard for Household Electric Storage... 174”), 53.01-10. (2) UL 1453, Standard for Electric Booster and Commercial Storage Tank Water Heaters...

46 CFR 53.01-1 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

.... (b) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY...., 333 Pfingston Road, Northbrook, IL 60062-2096: (1) UL 174, Standard for Household Electric Storage... 174”), 53.01-10. (2) UL 1453, Standard for Electric Booster and Commercial Storage Tank Water Heaters...

46 CFR 53.01-1 - Incorporation by reference.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) 2001... Road, Northbrook, IL 60062-2096: (1) UL 174, Standard for Household Electric Storage Tank Water Heaters...) UL 1453, Standard for Electric Booster and Commercial Storage Tank Water Heaters, Fourth Edition, Sep...

46 CFR 53.01-1 - Incorporation by reference.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) 2001... Road, Northbrook, IL 60062-2096: (1) UL 174, Standard for Household Electric Storage Tank Water Heaters...) UL 1453, Standard for Electric Booster and Commercial Storage Tank Water Heaters, Fourth Edition, Sep...

46 CFR 111.91-1 - Power, control, and interlock circuits.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 111.91-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Elevators and Dumbwaiters § 111.91-1 Power, control, and interlock circuits. Each electric power, control, and interlock circuit of an elevator or dumbwaiter must meet ASME...

46 CFR 111.91-1 - Power, control, and interlock circuits.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 111.91-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Elevators and Dumbwaiters § 111.91-1 Power, control, and interlock circuits. Each electric power, control, and interlock circuit of an elevator or dumbwaiter must meet ASME...

46 CFR 111.91-1 - Power, control, and interlock circuits.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 111.91-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Elevators and Dumbwaiters § 111.91-1 Power, control, and interlock circuits. Each electric power, control, and interlock circuit of an elevator or dumbwaiter must meet ASME...

46 CFR 111.91-1 - Power, control, and interlock circuits.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 111.91-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Elevators and Dumbwaiters § 111.91-1 Power, control, and interlock circuits. Each electric power, control, and interlock circuit of an elevator or dumbwaiter must meet ASME...

46 CFR 111.91-1 - Power, control, and interlock circuits.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 111.91-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Elevators and Dumbwaiters § 111.91-1 Power, control, and interlock circuits. Each electric power, control, and interlock circuit of an elevator or dumbwaiter must meet ASME...

Role of Friction in Materials Selection for Automotive Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Blau, Peter Julian

This is an invited article for a special issue of the ASM International monthly magazine that concerns "Automotive Materials and Applications." The article itself overviews frictional considerations in material selection for automobiles. It discusses implications for energy efficiency (engine friction) and safety (brakes) among other topics.

46 CFR 38.01-3 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

... incorporation by reference in this part, and the sections affected, are: American Society for Nondestructive...) American Society of Mechanical Engineers (ASME) International Three Park Avenue, New York, NY 10016-5990...) American Society for Testing and Materials (ASTM) 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959...

46 CFR 38.01-3 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

... incorporation by reference in this part, and the sections affected, are: American Society for Nondestructive...) American Society of Mechanical Engineers (ASME) International Three Park Avenue, New York, NY 10016-5990...) American Society for Testing and Materials (ASTM) 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959...

78 FR 23574 - Public Workshop on Marine Technology and Standards

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-19

... Technology and Standards AGENCY: Coast Guard, DHS. ACTION: Notice. SUMMARY: The American Society of... workshop on marine technology and standards in Arlington, VA. We originally published a notice regarding... Engineers/United States Coast Guard (ASME/USCG) Workshop on Marine Technology and Standards provides a...

Association of an SNP in a novel DREB2-like gene SiDREB2 with stress tolerance in foxtail millet [Setaria italica (L.)].

PubMed

Lata, Charu; Bhutty, Sarita; Bahadur, Ranjit Prasad; Majee, Manoj; Prasad, Manoj

2011-06-01

The DREB genes code for important plant transcription factors involved in the abiotic stress response and signal transduction. Characterization of DREB genes and development of functional markers for effective alleles is important for marker-assisted selection in foxtail millet. Here the characterization of a cDNA (SiDREB2) encoding a putative dehydration-responsive element-binding protein 2 from foxtail millet and the development of an allele-specific marker (ASM) for dehydration tolerance is reported. A cDNA clone (GenBank accession no. GT090998) coding for a putative DREB2 protein was isolated as a differentially expressed gene from a 6 h dehydration stress SSH library. A 5' RACE (rapid amplification of cDNA ends) was carried out to obtain the full-length cDNA, and sequence analysis showed that SiDREB2 encoded a polypeptide of 234 amino acids with a predicted mol. wt of 25.72 kDa and a theoretical pI of 5.14. A theoretical model of the tertiary structure shows that it has a highly conserved GCC-box-binding N-terminal domain, and an acidic C-terminus that acts as an activation domain for transcription. Based on its similarity to AP2 domains, SiDREB2 was classified into the A-2 subgroup of the DREB subfamily. Quantitative real-time PCR analysis showed significant up-regulation of SiDREB2 by dehydration (polyethylene glycol) and salinity (NaCl), while its expression was less affected by other stresses. A synonymous single nucleotide polymorphism (SNP) associated with dehydration tolerance was detected at the 558th base pair (an A/G transition) in the SiDREB2 gene in a core set of 45 foxtail millet accessions used. Based on the identified SNP, three primers were designed to develop an ASM for dehydration tolerance. The ASM produced a 261 bp fragment in all the tolerant accessions and produced no amplification in the sensitive accessions. The use of this ASM might be faster, cheaper, and more reproducible than other SNP genotyping methods, and thus will enable marker-aided breeding of foxtail millet for dehydration tolerance.

Air Separation Using Hollow Fiber Membranes

NASA Technical Reports Server (NTRS)

Huang, Stephen E.

2004-01-01

The NASA Glenn Research Center in partnership with the Ohio Aerospace Institute provides internship programs for high school and college students in the areas of science, engineering, professional administrative, and other technical areas. During the summer of 2004, I worked with Dr. Clarence T. Chang at NASA Glenn Research Center s combustion branch on air separation using hollow fiber membrane technology. . In light of the accident of Trans World Airline s flight 800, FAA has mandated that a suitable solution be created to prevent the ignition of fuel tanks in aircrafts. In order for any type of fuel to ignite, three important things are needed: fuel vapor, oxygen, and an energy source. Two different ways to make fuel tanks less likely to ignite are reformulating the fuel to obtain a lower vapor pressure for the fuel and or using an On Board Inert Gas Generating System (OBIGGS) to inert the Central Wing Tank. goal is to accomplish the mission, which means that the Air Separation Module (ASM) tends to be bulky and heavy. The primary goal for commercial aviation companies is to transport as much as they can with the least amount of cost and fuel per person, therefore the ASM must be compact and light as possible. The plan is to take bleed air from the aircraft s engines to pass air through a filter first to remove particulates and then pass the air through the ASM containing hollow fiber membranes. In the lab, there will be a heating element provided to simulate the temperature of the bleed air that will be entering the ASM and analysis of the separated air will be analyzed by a Gas Chromatograph/Mass Spectrometer (GC/MS). The GUMS will separate the different compounds in the exit streams of the ASM and provide information on the performance of hollow fiber membranes. Hopefully I can develop ways to improve efficiency of the ASM. different types of jet fuel were analyzed and data was well represented on SAE Paper 982485. Data consisted of the concentrations of over 300 different hydrocarbons commonly found in JP- 8, Jet A, and JP-5 fuels. I researched the major hydrocarbons that has a concentration of greater than 50 parts per million and found the vapor pressure data coefficients for a specific temperature range. The coefficients were applied to Antoine s Equation and Riedel's Equation to calculate the vapor pressures for that specific hydrocarbon in the specific temperature range. With the vapor pressure data scientists can formulate a fuel composition that has a lower vapor pressure profile, therefore making jet fuels less flammable. work, learn how to operate and examine the data from Gas Chromatograph and Mass Spectrometer, and develop new ways in applying hollow fiber membrane technology to other areas of environmental engineering. The United States military currently uses air separation technology and their primary The other side of making air travel safer is to reformulate the fuel. Analyses of three My goal this summer is to learn about hollow fiber membrane technologies and how they

46 CFR 53.01-1 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

...) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990...., 333 Pfingston Road, Northbrook, IL 60062-2096: (1) UL 174, Standard for Household Electric Storage... 174”), 53.01-10. (2) UL 1453, Standard for Electric Booster and Commercial Storage Tank Water Heaters...

46 CFR 54.01-5 - Scope (modifies U-1 and U-2).

Code of Federal Regulations, 2014 CFR

2014-10-01

... vessels must also comply with the requirements that are listed or prescribed in paragraphs (d) through (g... selected is Class I-L. (g) The design pressure for each interface between two chambers in a multichambered... BCategories C and D in accordance with UW-16 of section VIII of the ASME Boiler and Pressure Vessel Code Spot...

Advanced systems demonstration for utilization of biomass as an energy source. Volume 3: Equipment specifications

NASA Astrophysics Data System (ADS)

1980-10-01

Specifications are given for the shipping, marking, inspection, testing, and start up of equipment to be used in a proposed wood fuel cogeneration system in Maine. Couplings, mechanical drives, electric motors, spare parts, coatings, assembling, and materials handling and packaging are covered. Both OSHA and noise control regulations are included along with the ASME code.

FY17 Status Report on the Initial EPP Finite Element Analysis of Grade 91 Steel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Messner, M. C.; Sham, T. -L.

This report describes a modification to the elastic-perfectly plastic (EPP) strain limits design method to account for cyclic softening in Gr. 91 steel. The report demonstrates that the unmodified EPP strain limits method described in current ASME code case is not conservative for materials with substantial cyclic softening behavior like Gr. 91 steel. However, the EPP strain limits method can be modified to be conservative for softening materials by using softened isochronous stress-strain curves in place of the standard curves developed from unsoftened creep experiments. The report provides softened curves derived from inelastic material simulations and factors describing the transformationmore » of unsoftened curves to a softened state. Furthermore, the report outlines a method for deriving these factors directly from creep/fatigue tests. If the material softening saturates the proposed EPP strain limits method can be further simplified, providing a methodology based on temperature-dependent softening factors that could be implemented in an ASME code case allowing the use of the EPP strain limits method with Gr. 91. Finally, the report demonstrates the conservatism of the modified method when applied to inelastic simulation results and two bar experiments.« less

Activated sludge model (ASM) based modelling of membrane bioreactor (MBR) processes: a critical review with special regard to MBR specificities.

PubMed

Fenu, A; Guglielmi, G; Jimenez, J; Spèrandio, M; Saroj, D; Lesjean, B; Brepols, C; Thoeye, C; Nopens, I

2010-08-01

Membrane bioreactors (MBRs) have been increasingly employed for municipal and industrial wastewater treatment in the last decade. The efforts for modelling of such wastewater treatment systems have always targeted either the biological processes (treatment quality target) as well as the various aspects of engineering (cost effective design and operation). The development of Activated Sludge Models (ASM) was an important evolution in the modelling of Conventional Activated Sludge (CAS) processes and their use is now very well established. However, although they were initially developed to describe CAS processes, they have simply been transferred and applied to MBR processes. Recent studies on MBR biological processes have reported several crucial specificities: medium to very high sludge retention times, high mixed liquor concentration, accumulation of soluble microbial products (SMP) rejected by the membrane filtration step, and high aeration rates for scouring purposes. These aspects raise the question as to what extent the ASM framework is applicable to MBR processes. Several studies highlighting some of the aforementioned issues are scattered through the literature. Hence, through a concise and structured overview of the past developments and current state-of-the-art in biological modelling of MBR, this review explores ASM-based modelling applied to MBR processes. The work aims to synthesize previous studies and differentiates between unmodified and modified applications of ASM to MBR. Particular emphasis is placed on influent fractionation, biokinetics, and soluble microbial products (SMPs)/exo-polymeric substances (EPS) modelling, and suggestions are put forward as to good modelling practice with regard to MBR modelling both for end-users and academia. A last section highlights shortcomings and future needs for improved biological modelling of MBR processes. (c) 2010 Elsevier Ltd. All rights reserved.

Exploiting graph kernels for high performance biomedical relation extraction.

PubMed

Panyam, Nagesh C; Verspoor, Karin; Cohn, Trevor; Ramamohanarao, Kotagiri

2018-01-30

Relation extraction from biomedical publications is an important task in the area of semantic mining of text. Kernel methods for supervised relation extraction are often preferred over manual feature engineering methods, when classifying highly ordered structures such as trees and graphs obtained from syntactic parsing of a sentence. Tree kernels such as the Subset Tree Kernel and Partial Tree Kernel have been shown to be effective for classifying constituency parse trees and basic dependency parse graphs of a sentence. Graph kernels such as the All Path Graph kernel (APG) and Approximate Subgraph Matching (ASM) kernel have been shown to be suitable for classifying general graphs with cycles, such as the enhanced dependency parse graph of a sentence. In this work, we present a high performance Chemical-Induced Disease (CID) relation extraction system. We present a comparative study of kernel methods for the CID task and also extend our study to the Protein-Protein Interaction (PPI) extraction task, an important biomedical relation extraction task. We discuss novel modifications to the ASM kernel to boost its performance and a method to apply graph kernels for extracting relations expressed in multiple sentences. Our system for CID relation extraction attains an F-score of 60%, without using external knowledge sources or task specific heuristic or rules. In comparison, the state of the art Chemical-Disease Relation Extraction system achieves an F-score of 56% using an ensemble of multiple machine learning methods, which is then boosted to 61% with a rule based system employing task specific post processing rules. For the CID task, graph kernels outperform tree kernels substantially, and the best performance is obtained with APG kernel that attains an F-score of 60%, followed by the ASM kernel at 57%. The performance difference between the ASM and APG kernels for CID sentence level relation extraction is not significant. In our evaluation of ASM for the PPI task, ASM performed better than APG kernel for the BioInfer dataset, in the Area Under Curve (AUC) measure (74% vs 69%). However, for all the other PPI datasets, namely AIMed, HPRD50, IEPA and LLL, ASM is substantially outperformed by the APG kernel in F-score and AUC measures. We demonstrate a high performance Chemical Induced Disease relation extraction, without employing external knowledge sources or task specific heuristics. Our work shows that graph kernels are effective in extracting relations that are expressed in multiple sentences. We also show that the graph kernels, namely the ASM and APG kernels, substantially outperform the tree kernels. Among the graph kernels, we showed the ASM kernel as effective for biomedical relation extraction, with comparable performance to the APG kernel for datasets such as the CID-sentence level relation extraction and BioInfer in PPI. Overall, the APG kernel is shown to be significantly more accurate than the ASM kernel, achieving better performance on most datasets.

77 FR 26318 - Duke Energy Carolinas, LLC., Oconee Nuclear Station, Units 1, 2, and 3 Exemption

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-03

... the Transition Range,'' and American Society of Mechanical Engineers (ASME), Boiler and Pressure... heat numbers; (2) applied chemistry factors greater than 167 [deg]F (the weld wire heat- specific chemical composition, via the methodology of RG 1.99, Revision 2, indicated that higher chemistry factors...

Progress Report on Alloy 617 Time Dependent Allowables

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wright, Julie Knibloe

2015-06-01

Time dependent allowable stresses are required in the ASME Boiler and Pressure Vessel Code for design of components in the temperature range where time dependent deformation (i.e., creep) is expected to become significant. There are time dependent allowable stresses in Section IID of the Code for use in the non-nuclear construction codes, however, there are additional criteria that must be considered in developing time dependent allowables for nuclear components. These criteria are specified in Section III NH. St is defined as the lesser of three quantities: 100% of the average stress required to obtain a total (elastic, plastic, primary andmore » secondary creep) strain of 1%; 67% of the minimum stress to cause rupture; and 80% of the minimum stress to cause the initiation of tertiary creep. The values are reported for a range of temperatures and for time increments up to 100,000 hours. These values are determined from uniaxial creep tests, which involve the elevated temperature application of a constant load which is relatively small, resulting in deformation over a long time period prior to rupture. The stress which is the minimum resulting from these criteria is the time dependent allowable stress St. In this report data from a large number of creep and creep-rupture tests on Alloy 617 are analyzed using the ASME Section III NH criteria. Data which are used in the analysis are from the ongoing DOE sponsored high temperature materials program, form Korea Atomic Energy Institute through the Generation IV VHTR Materials Program and historical data from previous HTR research and vendor data generated in developing the alloy. It is found that the tertiary creep criterion determines St at highest temperatures, while the stress to cause 1% total strain controls at low temperatures. The ASME Section III Working Group on Allowable Stress Criteria has recommended that the uncertainties associated with determining the onset of tertiary creep and the lack of significant cavitation associated with early tertiary creep strain suggest that the tertiary creep criteria is not appropriate for this material. If the tertiary creep criterion is dropped from consideration, the stress to rupture criteria determines St at all but the lowest temperatures.« less

Highly Loaded Low-Pressure Turbine: Design, Numerical and Experimental Analysis (Preprint)

DTIC Science & Technology

2010-06-01

can be up 30 percent of the total weight of an aircraft engine [2], and may contain as many as 1 Copyright c⃝ 2010 by ASME 2000 individual airfoils ...reduction in stage count in a gas turbine engine and a de- crease in the part count of an individual airfoil row. The test data presented here provide...the vane row having modest design goals. So, while the Zweifel coefficient of the blade row was set Figure 1. Turbine design loop used to define the

Stress analysis and evaluation of a rectangular pressure vessel

NASA Astrophysics Data System (ADS)

Rezvani, M. A.; Ziada, H. H.; Shurrab, M. S.

1992-10-01

This study addresses structural analysis and evaluation of an abnormal rectangular pressure vessel, designed to house equipment for drilling and collecting samples from Hanford radioactive waste storage tanks. It had to be qualified according to ASME boiler and pressure vessel code, section 8; however, it had the cover plate bolted along the long face, a configuration not addressed by the code. Finite element method was used to calculate stresses resulting from internal pressure; these stresses were then used to evaluate and qualify the vessel. Fatigue is not a concern; thus, it can be built according to section 8, division 1 instead of division 2. Stress analysis was checked against the code. A stayed plate was added to stiffen the long side of the vessel.

Roles of the outer membrane protein AsmA of Salmonella enterica in the control of marRAB expression and invasion of epithelial cells.

PubMed

Prieto, Ana I; Hernández, Sara B; Cota, Ignacio; Pucciarelli, M Graciela; Orlov, Yuri; Ramos-Morales, Francisco; García-del Portillo, Francisco; Casadesús, Josep

2009-06-01

A genetic screen for suppressors of bile sensitivity in DNA adenine methylase (dam) mutants of Salmonella enterica serovar Typhimurium yielded insertions in an uncharacterized locus homologous to the Escherichia coli asmA gene. Disruption of asmA suppressed bile sensitivity also in phoP and wec mutants of S. enterica and increased the MIC of sodium deoxycholate for the parental strain ATCC 14028. Increased levels of marA mRNA were found in asmA, asmA dam, asmA phoP, and asmA wec strains of S. enterica, suggesting that lack of AsmA activates expression of the marRAB operon. Hence, asmA mutations may enhance bile resistance by inducing gene expression changes in the marRAB-controlled Mar regulon. In silico analysis of AsmA structure predicted the existence of one transmembrane domain. Biochemical analysis of subcellular fractions revealed that the asmA gene of S. enterica encodes a protein of approximately 70 kDa located in the outer membrane. Because AsmA is unrelated to known transport and/or efflux systems, we propose that activation of marRAB in asmA mutants may be a consequence of envelope reorganization. Competitive infection of BALB/c mice with asmA(+) and asmA isogenic strains indicated that lack of AsmA attenuates Salmonella virulence by the oral route but not by the intraperitoneal route. Furthermore, asmA mutants showed a reduced ability to invade epithelial cells in vitro.

Comparisons of ANS, ASME, AWS, and NFPA standards cited in the NRC standard review plan, NUREG-0800, and related documents

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ankrum, A.R.; Bohlander, K.L.; Gilbert, E.R.

This report provides the results of comparisons of the cited and latest versions of ANS, ASME, AWS and NFPA standards cited in the NRC Standard Review Plan for the Review of Safety Analysis Reports for Nuclear Power Plants (NUREG 0800) and related documents. The comparisons were performed by Battelle Pacific Northwest Laboratories in support of the NRC`s Standard Review Plan Update and Development Program. Significant changes to the standards, from the cited version to the latest version, are described and discussed in a tabular format for each standard. Recommendations for updating each citation in the Standard Review Plan are presented.more » Technical considerations and suggested changes are included for related regulatory documents (i.e., Regulatory Guides and the Code of Federal Regulations) citing the standard. The results and recommendations presented in this document have not been subjected to NRC staff review.« less

Multiaxial Creep-Fatigue and Creep-Ratcheting Failures of Grade 91 and Haynes 230 Alloys Toward Addressing Design Issues of Gen IV Nuclear Power Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hassan, Tasnim; Lissenden, Cliff; Carroll, Laura

The proposed research will develop systematic sets of uniaxial and multiaxial experimental data at a very high temperature (850-950°C) for Alloy 617. The loading histories to be prescribed in the experiments will induce creep-fatigue and creep-ratcheting failure mechanisms. These experimental responses will be scrutinized in order to quantify the influences of temperature and creep on fatigue and ratcheting failures. A unified constitutive model (UCM) will be developed and validated against these experimental responses. The improved UCM will be incorporated into the widely used finite element commercial software packages ANSYS. The modified ANSYS will be validated so that it can bemore » used for evaluating the very high temperature ASME-NH design-by-analysis methodology for Alloy 617 and thereby addressing the ASME-NH design code issues.« less

Progress Report on Alloy 617 Notched Specimen Testing

DOE Office of Scientific and Technical Information (OSTI.GOV)

McMurtrey, Michael David; Wright, Richard Neil; Lillo, Thomas Martin

Creep behavior of Alloy 617 has been extensively characterized to support the development of a draft Code Case to qualify Alloy 617 in Section III division 5 of the ASME Boiler and Pressure Vessel Code. This will allow use of Alloy 617 in construction of nuclear reactor components at elevated temperatures and longer periods of time (up to 950°C and 100,000 hours). Prior to actual use, additional concerns not considered in the ASME code need to be addressed. Code Cases are based largely on uniaxial testing of smooth gage specimens. In service conditions, components will generally be under multi axialmore » loading. There is also the concern of the behavior at discontinuities, such as threaded components. To address the concerns of multi axial creep behavior and at geometric discontinuities, notched specimens have been designed to create conditions representative of the states that service components experience. Two general notch geometries have been used for these series of tests: U notch and V notch specimens. The notches produce a tri axial stress state, though not uniform across the specimen. Characterization of the creep behavior of the U notch specimens and the creep rupture behavior of the V notch specimens provides a good approximation of the behavior expected of actual components. Preliminary testing and analysis have been completed and are reported in this document. This includes results from V notch specimens tested at 900°C and 800°C. Failure occurred in the smooth gage section of the specimen rather than at the root of the notch, though some damage was present at the root of the notch, where initial stress was highest. This indicates notch strengthening behavior in this material at these temperatures.« less

Functional Implications of Novel Human Acid Sphingomyelinase Splice Variants

PubMed Central

Rhein, Cosima; Tripal, Philipp; Seebahn, Angela; Konrad, Alice; Kramer, Marcel; Nagel, Christine; Kemper, Jonas; Bode, Jens; Mühle, Christiane; Gulbins, Erich; Reichel, Martin; Becker, Cord-Michael; Kornhuber, Johannes

2012-01-01

Background Acid sphingomyelinase (ASM) hydrolyses sphingomyelin and generates the lipid messenger ceramide, which mediates a variety of stress-related cellular processes. The pathological effects of dysregulated ASM activity are evident in several human diseases and indicate an important functional role for ASM regulation. We investigated alternative splicing as a possible mechanism for regulating cellular ASM activity. Methodology/Principal Findings We identified three novel ASM splice variants in human cells, termed ASM-5, -6 and -7, which lack portions of the catalytic- and/or carboxy-terminal domains in comparison to full-length ASM-1. Differential expression patterns in primary blood cells indicated that ASM splicing might be subject to regulatory processes. The newly identified ASM splice variants were catalytically inactive in biochemical in vitro assays, but they decreased the relative cellular ceramide content in overexpression studies and exerted a dominant-negative effect on ASM activity in physiological cell models. Conclusions/Significance These findings indicate that alternative splicing of ASM is of functional significance for the cellular stress response, possibly representing a mechanism for maintaining constant levels of cellular ASM enzyme activity. PMID:22558155

49 CFR 178.276 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2014 CFR

2014-10-01

....): (A) Without insulation or sun shield: 60 °C (140 °F); (B) With sun shield: 55 °C (131 °F); and (C) With insulation: 50 °C (122 °F). (3) Filling density means the average mass of liquefied compressed gas... stamped in accordance with the ASME Code, Section VIII. (2) Portable tanks must be postweld heat-treated...

49 CFR 178.276 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2012 CFR

2012-10-01

....): (A) Without insulation or sun shield: 60 °C (140 °F); (B) With sun shield: 55 °C (131 °F); and (C) With insulation: 50 °C (122 °F). (3) Filling density means the average mass of liquefied compressed gas... stamped in accordance with the ASME Code, Section VIII. (2) Portable tanks must be postweld heat-treated...

49 CFR 178.276 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2013 CFR

2013-10-01

....): (A) Without insulation or sun shield: 60 °C (140 °F); (B) With sun shield: 55 °C (131 °F); and (C) With insulation: 50 °C (122 °F). (3) Filling density means the average mass of liquefied compressed gas... stamped in accordance with the ASME Code, Section VIII. (2) Portable tanks must be postweld heat-treated...

49 CFR 178.276 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2010 CFR

2010-10-01

....): (A) Without insulation or sun shield: 60 °C (140 °F); (B) With sun shield: 55 °C (131 °F); and (C) With insulation: 50 °C (122 °F). (3) Filling density means the average mass of liquefied compressed gas... stamped in accordance with the ASME Code, Section VIII. (2) Portable tanks must be postweld heat-treated...

49 CFR 178.276 - Requirements for the design, construction, inspection and testing of portable tanks intended for...

Code of Federal Regulations, 2011 CFR

2011-10-01

....): (A) Without insulation or sun shield: 60 °C (140 °F); (B) With sun shield: 55 °C (131 °F); and (C) With insulation: 50 °C (122 °F). (3) Filling density means the average mass of liquefied compressed gas... stamped in accordance with the ASME Code, Section VIII. (2) Portable tanks must be postweld heat-treated...

Gas tungsten arc welding of aluminum alloys 6XXX. Welding procedure specification. Supplement 1. Records of procedure qualification tests. [6061 and 6063

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wodtke, C.H.; Frizzell, D.R.; Plunkett, W.A.

1986-06-01

Procedure WPS-1003 is qualified under Section IX of the ASME Boiler and Pressure Vessel Code for gas tungsten arc welding of aluminum alloys 6061 and 6063 (P-23), in thickness range 0.035 to 0.516 inch; filler metal is ER4043 (F-23) or ER5356 (F-22); shielding gas is argon.

Gas tungsten arc welding of aluminum alloys 3004, 5052, and 5X54. Welding procedure specification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wodtke, C.H.; Frizzell, D.R.; Plunkett, W.A.

1985-08-01

Procedure WPS-1002 is qualified under Section IX of the ASME Boiler and Pressure Vessel Code for gas tungsten arc welding of aluminum alloys 3004, 5052, 5154, and 5454 (P-22), in thickness range 0.062 to 0.5 in.; filler metal is ER4043 (F-23) for 3004, and ER5356 (F-22) for other alloys; shielding gas is argon.

Summary of Research 2000, Department of Aeronautics and Astronautics

DTIC Science & Technology

2001-12-01

swept transonic blading, and to facilitate design optimization; (iii) to install and test an advanced transonic axial stage, and thereby establish the...limited) rotor design optimization can now be attempted. (iii) The Sanger (code-validation) compressor stage was rebuilt, re- instrumented and retested...34Investigation of the Performance of a CFD Designed Compressor Stage," Paper AIAA 2000-3205, presented at the 36th AIAA/ASME/SAE/ASEE Joint Propulsion

Surface Analysis and Tools

NASA Technical Reports Server (NTRS)

Miyoshi, Kazuhisa

2002-01-01

This article is a chapter of the book entitled, "Tribology of Mechanical Systems," to be published by ASME Press, New York, NY. It describes selected analytical techniques, which are being used in understanding phenomena and mechanisms of oxidation, adhesion, bonding, friction, erosion, abrasion, and wear, and in defining the problems. The primary emphasis is on microanalytical approaches to engineering surfaces.

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

Effects of Fuel Specification and Additives on Soot Formation.

DTIC Science & Technology

1983-12-01

Engine Combustor Emissions," ASME 80-GT-70, 1980. 39. Naegeli , D.W. and Moses, C.A., "Effect of Fuel Molecular Structure on Soot Formation in Gas...Symposium on Combustion, pp. 1175-1183, The Combustion Institute, 1981. 108 41. Naegeli , D.W., Dodge, L.G. and Moses, C.A., "Effect of Flame Temperature

Closeout of IE Bulletin 83-05: ASME nuclear code pumps and spare parts manufactured by the Hayward Tyler Pump Company

DOE Office of Scientific and Technical Information (OSTI.GOV)

Foley, W.J.; Dean, R.S.; Hennick, A.

Documentation is provided in this report to close IE Bulletin 83-05 regarding ASME nuclear code pumps and spare parts manufactured by the Hayward Tyler Pump Company (HTPC). The bulletin was issued (1) to alert holders of operating licenses and construction permits of nuclear power plants that HTPC failed to implement effectively their quality assurance (QA) program from 1977 to 1981 and (2) to require affected utilities to take action to resolve the potential for failure of the subject pumps and their spare parts. Evaluation of utility responses and NRC/Region inspection reports shows that reliability of the affected pumps was ensuredmore » by means of procedures and performance testing of the pumps as required by the bulletin. Based on the evaluation, in accordance with specific criteria, the bulletin is closed for 116 (98%) of the 118 facilities to which it was issued for action and which were not shut down indefinitely or permanently at the time of issuance of this report. A follow-up item is proposed for the two facilities with open bulletin status. Based on favorable results, a conclusion is presented to indicate that the bulletin concerns have been resolved.« less

Mast cell migration to Th2 stimulated airway smooth muscle from asthmatics

PubMed Central

Sutcliffe, A; Kaur, D; Page, S; Woodman, L; Armour, C L; Baraket, M; Bradding, P; Hughes, J M; Brightling, C E

2006-01-01

Background Mast cell microlocalisation within the airway smooth muscle (ASM) bundle is an important determinant of the asthmatic phenotype. We hypothesised that mast cells migrate towards ASM in response to ASM derived chemokines. Methods Primary ASM cultures from subjects with and without asthma were stimulated with interleukin (IL)‐1β, IL‐4, and IL‐13 alone and in combination. Mast cell chemotaxis towards these ASM supernatants was investigated, and the chemotaxins mediating migration by using specific blocking antibodies for stem cell factor (SCF) and the chemokine receptors CCR3, CXCR1, 3 and 4 as well as the Gi inhibitor pertussis toxin and the tyrosine kinase inhibitor genistein were defined. The concentrations of CCL11, CXCL8, CXCL10, TGF‐β, and SCF in the supernatants were measured and the effect of non‐asthmatic ASM supernatants on the mast cell chemotactic activity of asthmatic ASM was examined. Results Human lung mast cells and HMC‐1 cells migrated towards Th2 stimulated ASM from asthmatics but not non‐asthmatics. Mast cell migration was mediated through the combined activation of CCR3 and CXCR1. CCL11 and CXCL8 expression by ASM increased markedly after stimulation, but was similar in those with and without asthma. ASM supernatants from non‐asthmatics inhibited mast cell migration towards the asthmatic ASM supernatant. Conclusion Th2 stimulated ASM from asthmatics is chemotactic for mast cells. Non‐asthmatic ASM releases a mediator or mediators that inhibit mast cell migration towards stimulated asthmatic ASM. Specifically targeting mast cell migration into the ASM bundle may provide a novel treatment for asthma. PMID:16601090

Orosomucoid-like 3 (ORMDL3) upregulates airway smooth muscle proliferation, contraction, and Ca2+ oscillations in asthma.

PubMed

Chen, Jun; Miller, Marina; Unno, Hirotoshi; Rosenthal, Peter; Sanderson, Michael J; Broide, David H

2017-09-07

Airway hyperresponsiveness is a major feature of asthma attributed predominantly to an extrinsic immune/inflammatory response increasing airway smooth muscle (ASM) contractility. We investigated whether increased ASM expression of orosomucoid-like 3 (ORMDL3), a gene on chromosome 17q21 highly linked to asthma, induced increased ASM proliferation and contractility in vitro and influenced airway contractility and calcium flux in ASM in precision-cut lung slices (PCLSs) from wild-type and hORMDL3 Zp3-Cre mice (which express increased levels of human ORMDL3 [hORMDL3]). Levels of ASM proliferation and contraction were assessed in ASM cells transfected with ORMDL3 in vitro. In addition, airway contractility and calcium oscillations were quantitated in ASM cells in PCLSs derived from naive wild-type and naive hORMDL3 Zp3-Cre mice, which do not have a blood supply. Increased ASM expression of ORMDL3 in vitro resulted in increased ASM proliferation and contractility. PCLSs derived from naive hORMDL3 Zp3-Cre mice, which do not have airway inflammation, exhibit increased airway contractility with increased calcium oscillations in ASM cells. Increased ASM ORMDL3 expression increases levels of ASM sarcoplasmic reticulum Ca 2+ ATPase 2b (SERCA2b), which increases ASM proliferation and contractility. Overall, these studies provide evidence that an intrinsic increase in ORMDL3 expression in ASM can induce increased ASM proliferation and contractility, which might contribute to increased airway hyperresponsiveness in the absence of airway inflammation in asthmatic patients. Copyright © 2017 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

β2-Agonist Induced cAMP Is Decreased in Asthmatic Airway Smooth Muscle Due to Increased PDE4D

PubMed Central

Trian, Thomas; Burgess, Janette K.; Niimi, Kyoko; Moir, Lyn M.; Ge, Qi; Berger, Patrick; Liggett, Stephen B.; Black, Judith L.; Oliver, Brian G.

2011-01-01

Background and Objective Asthma is associated with airway narrowing in response to bronchoconstricting stimuli and increased airway smooth muscle (ASM) mass. In addition, some studies have suggested impaired β-agonist induced ASM relaxation in asthmatics, but the mechanism is not known. Objective To characterize the potential defect in β-agonist induced cAMP in ASM derived from asthmatic in comparison to non-asthmatic subjects and to investigate its mechanism. Methods We examined β2-adrenergic (β2AR) receptor expression and basal β-agonist and forskolin (direct activator of adenylyl cyclase) stimulated cAMP production in asthmatic cultured ASM (n = 15) and non-asthmatic ASM (n = 22). Based on these results, PDE activity, PDE4D expression and cell proliferation were determined. Results In the presence of IBMX, a pan PDE inhibitor, asthmatic ASM had ∼50% lower cAMP production in response to isoproterenol, albuterol, formoterol, and forskolin compared to non-asthmatic ASM. However when PDE4 was specifically inhibited, cAMP production by the agonists and forskolin was normalized in asthmatic ASM. We then measured the amount and activity of PDE4, and found ∼2-fold greater expression and activity in asthmatic ASM compared to non-asthmatic ASM. Furthermore, inhibition of PDE4 reduced asthmatic ASM proliferation but not that of non-asthmatic ASM. Conclusion Decreased β-agonist induced cAMP in ASM from asthmatics results from enhanced degradation due to increased PDE4D expression. Clinical manifestations of this dysregulation would be suboptimal β-agonist-mediated bronchodilation and possibly reduced control over increasing ASM mass. These phenotypes appear to be “hard-wired” into ASM from asthmatics, as they do not require an inflammatory environment in culture to be observed. PMID:21611147

14 CFR 330.45 - What is the basis on which air carriers will be compensated under the set-aside?

Code of Federal Regulations, 2010 CFR

2010-01-01

... be calculated using a fixed ASM rate equivalent to the mean losses per ASM for all Class I carriers... graduated ASM rate equivalent to— (i) The mean loss per ASM for all Class I carriers applying for compensation, for each of the first 75,000 ASMs reported; and (ii) The mean remaining loss per ASM for all...

Phenotype and Functional Features of Human Telomerase Reverse Transcriptase Immortalized Human Airway Smooth Muscle Cells from Asthmatic and Non-Asthmatic Donors.

PubMed

Burgess, J K; Ketheson, A; Faiz, A; Limbert Rempel, K A; Oliver, B G; Ward, J P T; Halayko, A J

2018-01-16

Asthma is an obstructive respiratory disease characterised by chronic inflammation with airway hyperresponsiveness. In asthmatic airways, there is an increase in airway smooth muscle (ASM) cell bulk, which differs from non-asthmatic ASM in characteristics. This study aimed to assess the usefulness of hTERT immortalisation of human ASM cells as a research tool. Specifically we compared proliferative capacity, inflammatory mediator release and extracellular matrix (ECM) production in hTERT immortalised and parent primary ASM cells from asthmatic and non-asthmatic donors. Our studies revealed no significant differences in proliferation, IL-6 and eotaxin-1 production, or CTGF synthesis between donor-matched parent and hTERT immortalised ASM cell lines. However, deposition of ECM proteins fibronectin and fibulin-1 was significantly lower in immortalised ASM cells compared to corresponding primary cells. Notably, previously reported differences in proliferation and inflammatory mediator release between asthmatic and non-asthmatic ASM cells were retained, but excessive ECM protein deposition in asthmatic ASM cells was lost in hTERT ASM cells. This study shows that hTERT immortalised ASM cells mirror primary ASM cells in proliferation and inflammatory profile characteristics. Moreover, we demonstrate both strengths and weaknesses of this immortalised cell model as a representation of primary ASM cells for future asthma pathophysiological research.

Allele-Skewed DNA Modification in the Brain: Relevance to a Schizophrenia GWAS

PubMed Central

Gagliano, Sarah A.; Ptak, Carolyn; Mak, Denise Y.F.; Shamsi, Mehrdad; Oh, Gabriel; Knight, Joanne; Boutros, Paul C.; Petronis, Arturas

2016-01-01

Numerous recent studies have suggested that phenotypic effects of DNA sequence variants can be mediated or modulated by their epigenetic marks, such as allele-skewed DNA modification (ASM). Using Affymetrix SNP microarrays, we performed a comprehensive search of ASM effects in human post-mortem brain and sperm samples (total n = 256) from individuals with major psychosis and control individuals. Depending on the phenotypic category of the brain samples, 1.4%–7.5% of interrogated SNPs exhibited ASM effects. Next, we investigated ASM in the context of genetic studies of schizophrenia and detected that brain ASM SNPs were significantly overrepresented among sub-threshold SNPs from a schizophrenia genome-wide association study (GWAS). Brain ASM SNPs showed a much stronger enrichment in a schizophrenia GWAS than in 17 large GWASs of non-psychiatric diseases and traits, arguing that ASM effects are at least partially tissue specific. Studies of germline and control brain ASM SNPs supported a causal association between ASM and schizophrenia. Finally, significantly higher proportions of ASM SNPs than of non-ASM SNPs were detected at loci exhibiting epigenetic signatures of enhancers and promoters, and they were overrepresented within transcription factor binding regions and DNase I hypersensitive sites. All of these findings collectively indicate that ASM SNPs should be prioritized in follow-up GWASs. PMID:27087318

The DNA Methylome of Human Peripheral Blood Mononuclear Cells

PubMed Central

Ye, Mingzhi; Zheng, Hancheng; Yu, Jian; Wu, Honglong; Sun, Jihua; Zhang, Hongyu; Chen, Quan; Luo, Ruibang; Chen, Minfeng; He, Yinghua; Jin, Xin; Zhang, Qinghui; Yu, Chang; Zhou, Guangyu; Sun, Jinfeng; Huang, Yebo; Zheng, Huisong; Cao, Hongzhi; Zhou, Xiaoyu; Guo, Shicheng; Hu, Xueda; Li, Xin; Kristiansen, Karsten; Bolund, Lars; Xu, Jiujin; Wang, Wen; Yang, Huanming; Wang, Jian; Li, Ruiqiang; Beck, Stephan; Wang, Jun; Zhang, Xiuqing

2010-01-01

DNA methylation plays an important role in biological processes in human health and disease. Recent technological advances allow unbiased whole-genome DNA methylation (methylome) analysis to be carried out on human cells. Using whole-genome bisulfite sequencing at 24.7-fold coverage (12.3-fold per strand), we report a comprehensive (92.62%) methylome and analysis of the unique sequences in human peripheral blood mononuclear cells (PBMC) from the same Asian individual whose genome was deciphered in the YH project. PBMC constitute an important source for clinical blood tests world-wide. We found that 68.4% of CpG sites and <0.2% of non-CpG sites were methylated, demonstrating that non-CpG cytosine methylation is minor in human PBMC. Analysis of the PBMC methylome revealed a rich epigenomic landscape for 20 distinct genomic features, including regulatory, protein-coding, non-coding, RNA-coding, and repeat sequences. Integration of our methylome data with the YH genome sequence enabled a first comprehensive assessment of allele-specific methylation (ASM) between the two haploid methylomes of any individual and allowed the identification of 599 haploid differentially methylated regions (hDMRs) covering 287 genes. Of these, 76 genes had hDMRs within 2 kb of their transcriptional start sites of which >80% displayed allele-specific expression (ASE). These data demonstrate that ASM is a recurrent phenomenon and is highly correlated with ASE in human PBMCs. Together with recently reported similar studies, our study provides a comprehensive resource for future epigenomic research and confirms new sequencing technology as a paradigm for large-scale epigenomics studies. PMID:21085693

Characterization of Acid Sphingomyelinase Activity in Human Cerebrospinal Fluid

PubMed Central

Mühle, Christiane; Huttner, Hagen B.; Walter, Silke; Reichel, Martin; Canneva, Fabio; Lewczuk, Piotr; Gulbins, Erich; Kornhuber, Johannes

2013-01-01

Background As a key enzyme in sphingolipid metabolism, acid sphingomyelinase (ASM) is involved in the regulation of cell fate and signaling via hydrolysis of sphingomyelin to form ceramide. While increased activity of the lysosomal form has been associated with various pathological conditions, there are few studies on secretory ASM limited only to cell models, plasma or serum. Methods An optimized assay based on a fluorescent substrate was applied to measure the ASM activity in cerebrospinal fluid (CSF) collected from mice and from 42 patients who were classified as controls based on normal routine CSF values. Results We have detected ASM activity in human CSF, established a sensitive quantitative assay and characterized the enzyme’s properties. The enzyme resembles plasmatic ASM including protein stability and Zn2+-dependence but the assays differ considerably in the optimal detergent concentration. Significantly increased activities in the CSF of ASM transgenic mice and undetectable levels in ASM knock-out mice prove that the measured ASM activity originates from the ASM-encoding gene SMPD1. CSF localized ASM activities were comparable to corresponding serum ASM levels at their respective optimal reaction conditions, but no correlation was observed. The large variance in ASM activity was independent of sex, age or analyzed routine CSF parameters. Conclusions Human and mouse CSF contain detectable levels of secretory ASM, which are unrelated to serum ASM activities. Further investigations in humans and in animal models will help to elucidate the role of this enzyme in human disease and to assess its value as a potential biomarker for disease type, severity, progress or therapeutic success. PMID:23658784

Using SA508/533 for the HTGR Vessel Material

DOE Office of Scientific and Technical Information (OSTI.GOV)

Larry Demick

2012-06-01

This paper examines the influence of High Temperature Gas-cooled Reactor (HTGR) module power rating and normal operating temperatures on the use of SA508/533 material for the HTGR vessel system with emphasis on the calculated times at elevated temperatures approaching or exceeding ASME Code Service Limits (Levels B&C) to which the reactor pressure vessel could be exposed during postulated pressurized and depressurized conduction cooldown events over its design lifetime.

Military Families In Transition: Stress, Resilience, And Well-Being

DTIC Science & Technology

2014-01-01

Smith College of Engineering and Computer Science Professor, Department of Physics , College of Arts and Sciences Fellow: AIAA, ASME, APS, Institute...of Physics (UK) Syracuse University Richard E. Heyman, PhD Professor Family Translational Research Group Department of Cariology and Comprehensive...Pasquina, MD COL(R), USA Residency Director and Chair, Physical Medicine & Rehabilitation Uniformed Services University Walter Reed National

40 CFR 60.4415 - How do I conduct the initial and subsequent performance tests for sulfur?

Code of Federal Regulations, 2013 CFR

2013-07-01

..., 8, or 20 in appendix A of this part. In addition, the American Society of Mechanical Engineers (ASME... appendix A of this part, and measure and record the electrical and thermal output from the unit. Then use... the run, in ppm Qstd = stack gas volumetric flow rate, in dscf/hr P = gross electrical and mechanical...

40 CFR 60.4415 - How do I conduct the initial and subsequent performance tests for sulfur?

Code of Federal Regulations, 2014 CFR

2014-07-01

..., 8, or 20 in appendix A of this part. In addition, the American Society of Mechanical Engineers (ASME... appendix A of this part, and measure and record the electrical and thermal output from the unit. Then use... the run, in ppm Qstd = stack gas volumetric flow rate, in dscf/hr P = gross electrical and mechanical...

40 CFR 60.4415 - How do I conduct the initial and subsequent performance tests for sulfur?

Code of Federal Regulations, 2012 CFR

2012-07-01

..., 8, or 20 in appendix A of this part. In addition, the American Society of Mechanical Engineers (ASME... appendix A of this part, and measure and record the electrical and thermal output from the unit. Then use... the run, in ppm Qstd = stack gas volumetric flow rate, in dscf/hr P = gross electrical and mechanical...

40 CFR 60.4415 - How do I conduct the initial and subsequent performance tests for sulfur?

Code of Federal Regulations, 2010 CFR

2010-07-01

..., 8, or 20 in appendix A of this part. In addition, the American Society of Mechanical Engineers (ASME... appendix A of this part, and measure and record the electrical and thermal output from the unit. Then use... the run, in ppm Qstd = stack gas volumetric flow rate, in dscf/hr P = gross electrical and mechanical...

40 CFR 60.4415 - How do I conduct the initial and subsequent performance tests for sulfur?

Code of Federal Regulations, 2011 CFR

2011-07-01

..., 8, or 20 in appendix A of this part. In addition, the American Society of Mechanical Engineers (ASME... appendix A of this part, and measure and record the electrical and thermal output from the unit. Then use... the run, in ppm Qstd = stack gas volumetric flow rate, in dscf/hr P = gross electrical and mechanical...

46 CFR 56.07-10 - Design conditions and criteria (modifies 101-104.7).

Code of Federal Regulations, 2010 CFR

2010-10-01

... ENGINEERING PIPING SYSTEMS AND APPURTENANCES Design § 56.07-10 Design conditions and criteria (modifies 101... system must not be greater than the internal design pressure defined in 104.1.2 of ASME B31.1 (incorporated by reference; see 46 CFR 56.01-2). (2) Where the maximum allowable working pressure of a system...

NREL's National Wind Technology Center Director Named ASME Fellow

Science.gov Websites

Division before becoming founding director of the NWTC. He joined NREL in 1984 following a 14-year stint as members with at least 10 years of engineering practice who have made "significant contributions" Monrad, (303) 275-4096 Golden, Colo., January 25, 1996 -- Dr. Robert W. Thresher, director of the

An adaptive sampling method for variable-fidelity surrogate models using improved hierarchical kriging

NASA Astrophysics Data System (ADS)

Hu, Jiexiang; Zhou, Qi; Jiang, Ping; Shao, Xinyu; Xie, Tingli

2018-01-01

Variable-fidelity (VF) modelling methods have been widely used in complex engineering system design to mitigate the computational burden. Building a VF model generally includes two parts: design of experiments and metamodel construction. In this article, an adaptive sampling method based on improved hierarchical kriging (ASM-IHK) is proposed to refine the improved VF model. First, an improved hierarchical kriging model is developed as the metamodel, in which the low-fidelity model is varied through a polynomial response surface function to capture the characteristics of a high-fidelity model. Secondly, to reduce local approximation errors, an active learning strategy based on a sequential sampling method is introduced to make full use of the already required information on the current sampling points and to guide the sampling process of the high-fidelity model. Finally, two numerical examples and the modelling of the aerodynamic coefficient for an aircraft are provided to demonstrate the approximation capability of the proposed approach, as well as three other metamodelling methods and two sequential sampling methods. The results show that ASM-IHK provides a more accurate metamodel at the same simulation cost, which is very important in metamodel-based engineering design problems.

ADAPTION OF NONSTANDARD PIPING COMPONENTS INTO PRESENT DAY SEISMIC CODES

DOE Office of Scientific and Technical Information (OSTI.GOV)

D. T. Clark; M. J. Russell; R. E. Spears

2009-07-01

With spiraling energy demand and flat energy supply, there is a need to extend the life of older nuclear reactors. This sometimes requires that existing systems be evaluated to present day seismic codes. Older reactors built in the 1960s and early 1970s often used fabricated piping components that were code compliant during their initial construction time period, but are outside the standard parameters of present-day piping codes. There are several approaches available to the analyst in evaluating these non-standard components to modern codes. The simplest approach is to use the flexibility factors and stress indices for similar standard components withmore » the assumption that the non-standard component’s flexibility factors and stress indices will be very similar. This approach can require significant engineering judgment. A more rational approach available in Section III of the ASME Boiler and Pressure Vessel Code, which is the subject of this paper, involves calculation of flexibility factors using finite element analysis of the non-standard component. Such analysis allows modeling of geometric and material nonlinearities. Flexibility factors based on these analyses are sensitive to the load magnitudes used in their calculation, load magnitudes that need to be consistent with those produced by the linear system analyses where the flexibility factors are applied. This can lead to iteration, since the magnitude of the loads produced by the linear system analysis depend on the magnitude of the flexibility factors. After the loading applied to the nonstandard component finite element model has been matched to loads produced by the associated linear system model, the component finite element model can then be used to evaluate the performance of the component under the loads with the nonlinear analysis provisions of the Code, should the load levels lead to calculated stresses in excess of Allowable stresses. This paper details the application of component-level finite element modeling to account for geometric and material nonlinear component behavior in a linear elastic piping system model. Note that this technique can be applied to the analysis of B31 piping systems.« less

Creep of A508/533 Pressure Vessel Steel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Richard Wright

2014-08-01

ABSTRACT Evaluation of potential Reactor Pressure Vessel (RPV) steels has been carried out as part of the pre-conceptual Very High Temperature Reactor (VHTR) design studies. These design studies have generally focused on American Society of Mechanical Engineers (ASME) Code status of the steels, temperature limits, and allowable stresses. Initially, three candidate materials were identified by this process: conventional light water reactor (LWR) RPV steels A508 and A533, 2¼Cr-1Mo in the annealed condition, and Grade 91 steel. The low strength of 2¼Cr-1Mo at elevated temperature has eliminated this steel from serious consideration as the VHTR RPV candidate material. Discussions with themore » very few vendors that can potentially produce large forgings for nuclear pressure vessels indicate a strong preference for conventional LWR steels. This preference is based in part on extensive experience with forging these steels for nuclear components. It is also based on the inability to cast large ingots of the Grade 91 steel due to segregation during ingot solidification, thus restricting the possible mass of forging components and increasing the amount of welding required for completion of the RPV. Grade 91 steel is also prone to weld cracking and must be post-weld heat treated to ensure adequate high-temperature strength. There are also questions about the ability to produce, and very importantly, verify the through thickness properties of thick sections of Grade 91 material. The availability of large components, ease of fabrication, and nuclear service experience with the A508 and A533 steels strongly favor their use in the RPV for the VHTR. Lowering the gas outlet temperature for the VHTR to 750°C from 950 to 1000°C, proposed in early concept studies, further strengthens the justification for this material selection. This steel is allowed in the ASME Boiler and Pressure Vessel Code for nuclear service up to 371°C (700°F); certain excursions above that temperature are allowed by Code Case N-499-2 (now incorporated as an appendix to Section III Division 5 of the Code). This Code Case was developed with a rather sparse data set and focused primarily on rolled plate material (A533 specification). Confirmatory tests of creep behavior of both A508 and A533 are described here that are designed to extend the database in order to build higher confidence in ensuring the structural integrity of the VHTR RPV during off-normal conditions. A number of creep-rupture tests were carried out at temperatures above the 371°C (700°F) Code limit; longer term tests designed to evaluate minimum creep behavior are ongoing. A limited amount of rupture testing was also carried out on welded material. All of the rupture data from the current experiments is compared to historical values from the testing carried out to develop Code Case N-499-2. It is shown that the A508/533 basemetal tested here fits well with the rupture behavior reported from the historical testing. The presence of weldments significantly reduces the time to rupture. The primary purpose of this report is to summarize and record the experimental results in a single document.« less

Exposure of a liquefied gas container to an external fire.

PubMed

Raj, Phani K

2005-06-30

In liquefied gas, bulk-storage facilities and plants, the separation distances between storage tanks and between a tank and a line of adjoining property that can be built are governed by local regulations and/or codes (e.g. National Fire Protection Association (NFPA) 58, 2004). Separation distance requirements have been in the NFPA 58 Code for over 60 years; however, no scientific foundations (either theoretical or experimental) are available for the specified distances. Even though the liquefied petroleum gas (LPG) industry has operated safely over the years, there is a question as to whether the code-specified distances provide sufficient safety to LPG-storage tanks, when they are exposed to large external fires. A radiation heat-transfer-based model is presented in this paper. The temporal variation of the vapor-wetted tank-wall temperature is calculated when exposed to thermal radiation from an external, non-impinging, large, 30.5 m (100 ft) diameter, highly radiative, hydrocarbon fuel (pool) fire located at a specified distance. Structural steel wall of a pressurized, liquefied gas container (such as the ASME LP-Gas tank) begins to lose its strength, when the wall temperature approaches a critical temperature, 810 K (1000 degrees F). LP-Gas tank walls reaching close to this temperature will be a cause for major concern because of increased potential for tank failure, which could result in catastrophic consequences. Results from the model for exposure of different size ASME (LP-Gas) containers to a hydrocarbon pool fire of 30.5 m (100 ft) in diameter, located with its base edge at the separation distances specified by NFPA 58 [NFPA 58, Liquefied Petroleum Gas Code, Table 6.3.1, 2004 ed., National Fire Protection Association, Quincy, MA, 2004] indicate that the vapor-wetted wall temperature of the containers never reach the critical temperature under common wind conditions (0, 5 and 10 m/s), with the flame tilting towards the tank. This indicates that the separation distances specified in the code are adequate for non-impingement type of fires. The model can be used to test the efficacy of other similar codes and regulations for other materials.

Knee cartilage segmentation using active shape models and local binary patterns

NASA Astrophysics Data System (ADS)

González, Germán.; Escalante-Ramírez, Boris

2014-05-01

Segmentation of knee cartilage has been useful for opportune diagnosis and treatment of osteoarthritis (OA). This paper presents a semiautomatic segmentation technique based on Active Shape Models (ASM) combined with Local Binary Patterns (LBP) and its approaches to describe the surrounding texture of femoral cartilage. The proposed technique is tested on a 16-image database of different patients and it is validated through Leave- One-Out method. We compare different segmentation techniques: ASM-LBP, ASM-medianLBP, and ASM proposed by Cootes. The ASM-LBP approaches are tested with different ratios to decide which of them describes the cartilage texture better. The results show that ASM-medianLBP has better performance than ASM-LBP and ASM. Furthermore, we add a routine which improves the robustness versus two principal problems: oversegmentation and initialization.

Design and evaluation of a high temperature/pressure supercritical carbon dioxide direct tubular receiver for concentrating solar power applications

NASA Astrophysics Data System (ADS)

Ortega, Jesus Daniel

This work focuses on the development of a solar power thermal receiver for a supercritical-carbon dioxide (sCO2), Brayton power-cycle to produce ~1 MWe. Closed-loop sCO2 Brayton cycles are being evaluated in combination with concentrating solar power to provide higher thermal-to-electric conversion efficiencies relative to conventional steam Rankine cycles. High temperatures (923--973 K) and pressures (20--25 MPa) are required in the solar receiver to achieve thermal efficiencies of ~50%, making concentrating solar power (CSP) technologies a competitive alternative to current power generation methods. In this study, the CSP receiver is required to achieve an outlet temperature of 923 K at 25 MPa or 973 K at 20 MPa to meet the operating needs. To obtain compatible receiver tube material, an extensive material review was performed based the ASME Boiler and Pressure Vessel Code, ASME B31.1 and ASME B313.3 codes respectively. Subsequently, a thermal-structural model was developed using a commercial computational fluid (CFD) dynamics and structural mechanics software for designing and analyzing the tubular receiver that could provide the heat input for a ~2 MWth plant. These results were used to perform an analytical cumulative damage creep-fatigue analysis to estimate the work-life of the tubes. In sequence, an optical-thermal-fluid model was developed to evaluate the resulting thermal efficiency of the tubular receiver from the NSTTF heliostat field. The ray-tracing tool SolTrace was used to obtain the heat-flux distribution on the surfaces of the receiver. The K-ω SST turbulence model and P-1 radiation model used in Fluent were coupled with SolTrace to provide the heat flux distribution on the receiver surface. The creep-fatigue analysis displays the damage accumulated due to the cycling and the permanent deformation of the tubes. Nonetheless, they are able to support the required lifetime. The receiver surface temperatures were found to be within the safe operational limit while exhibiting a receiver thermal efficiency of ~85%. Future work includes the completion of a cyclic loading analysis to be performed using the Larson-Miller creep model in nCode Design Life to corroborate the structural integrity of the receiver over the desired lifetime of ~10,000 cycles.

Corrosion fatigue of alloys 600 and 690 in simulated LWR environments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ruther, W.E.; Soppett, W.K.; Kassner, T.F.

1996-04-01

Crack growth data were obtained on fracture-mechanics specimens of Alloys 600 and 690 to investigate environmentally assisted cracking (EAC) in simulated boiling water reactor and pressurized water reactor environments at 289 and 320 C. Preliminary information was obtained on the effect of temperature, load ratio, stress intensity (K), and the dissolved-oxygen and -hydrogen concentrations of the water on EAC. Specimens of Type 316NG and sensitized Type 304 stainless steel (SS) were included in several of the experiments to assess the behavior of these materials and Alloy 600 under the same water chemistry and loading conditions. The experimental data are comparedmore » with predictions from an Argonne National Laboratory (ANL) model for crack growth rates (CGRs) of SSs in water and the ASME Code Section 11 correlation for CGRs in air at the K{sub max} and load-ratio values in the various tests. The data for all of the materials were bounded by ANL model predictions and the ASME Section 11 ``air line.``« less

Creep-Rupture Behavior and Recrystallization in HR6W and Haynes Alloy 230 Cold-Bent Boiler Tubing for Ultrasupercritical (USC) Steam Boiler Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shingledecker, John P

2007-01-01

Creep-rupture experiments were conducted on HR6W and Haynes 230, candidate Ultrasupercritical (USC) alloys, tubes to evaluate the effects of cold-work and recrystallization during high-temperature service. These creep tests were performed by internally pressurizing cold-bent boiler tubes at 775 C for times up to 8000 hours. The bends were fabricated with cold-work levels beyond the current ASME Boiler and Pressure Vessel (ASME B&PV) Code Section I limits for austenitic stainless steels. Destructive metallographic evaluation of the crept tube bends was used to determine the effects of cold-work and the degree of recrystallization. The metallographic analysis combined with an evaluation of themore » creep and rupture data suggest that solid-solution strengthened nickel-based alloys can be fabricated for high-temperature service at USC conditions utilizing levels of cold-work higher than the current allowed levels for austenitic stainless steels.« less

Engineering studies of vectorcardiographs in blood pressure measuring systems, appendix 3

NASA Technical Reports Server (NTRS)

Mark, R. G.

1975-01-01

The following subjects were covered: (1) ASM80 manual, (2) signal preprocessing as an aid to on-line EKG analysis, and (3) high speed evaluation of magnetic tape recordings of electrocardiograms. A description of the ASM80 symbolic assembly program for the INTEL 8080 microprocessor and a user's manual were presented. The capability of three redundancy reduction algorithms to produce adequate representations of electrocardiographic data was examined. A hardware device was constructed which carried out zero order interpolation on a signal. Examination of the zero order interpolators reconstructed signal indicated that this representation was adequate for analysis of rhythm. A system to analyze magnetic tapes of electrocardiograms recorded over 24 hour intervals was designed. The recordings are sampled 200 times per second using a Nova computer and a special interface system. This system was tested on several recordings of clinical data, containing over 75 premature ventricular contractions, each one of which was flagged.

Welding procedure specification. Supplement 1. Records of procedure qualification tests. Gas tungsten arc welding of aluminum alloys 3004, 5052, and 5X54

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wodtke, C.H.; Frizzell, D.R.; Plunkett, W.A.

1986-06-01

Procedure WPS-1002 is qualified under Section IX of the ASME Boiler and Pressure Vessel Code for gas tungsten arc welding of aluminum alloys 3004, 5052, 5154, and 5454 (P-22), in thickness range 0.062 to 0.5 inches; filler metal is ER4043 (F-23) for 3004, and ER5356 (F-22) for other alloys; shielding gas is argon.

The Shock and Vibration Digest. Volume 12, Number 10,

DTIC Science & Technology

1980-10-01

according to the various behavior stress limits of the ASME code. 80-2289 Tranmition of an Oscillating Incompressible Flow in a Pipe or in a Boundary...a Normal Shock Wave Contained limits are also presented, in an Aerodynamic Inlet ( Pulsation d’un Choc Droit en Aerodynamique Interne) A. Agnes, E...Department of Defense applauded this effort and called for lAG operations that are strongly oriented toward user needs. This is very encouraging, but

Evaluation on the Feasibility of Using Ultrasonic Testing of Reactor Pressure Vessel Welds for Assessing Flaw Density/Distribution per 10 CFR 50.61a, Alternate Fracture Toughness Requirements for Protection Against Pressurized Thermal Shock

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sullivan, Edmund J.; Anderson, Michael T.

2014-06-10

This technical letter report provides the status of an assessment undertaken by PNNL at the request of the NRC to verify the capability of periodic ASME-required volumetric examinations of reactor vessels to characterize the density and distribution of flaws of interest for applying §50.61a on a plant-by-plant basis. The PTS rule, described in the Code of Federal Regulations, Title 10, Section 50.61 (§50.61), "Fracture Toughness Requirements for Protection against Pressurized Thermal Shock Events," establishes screening criteria to ensure that the potential for a reactor vessel to fail due to a PTS event is deemed to be acceptably low. Recently, themore » NRC completed a research program that concluded that the risk of through-wall cracking due to a PTS event is much lower than previously estimated. The NRC subsequently developed and promulgated an alternate PTS rule, §50.61a, that can be implemented by PWR licensees. The §50.61a rule differs from §50.61 in that it requires licensees who choose to follow this alternate method to analyze the results from periodic volumetric examinations required by the ASME Code, Section XI, Rules for Inservice Inspection (ISI) of Nuclear Power Plants.« less

Measurement and Analysis of the Periodic Variation of Total Pressure in an Axial-Flow Compressor Stage.

DTIC Science & Technology

1980-11-01

u r N. rCOL.16 JA Eld Ps of@c 1394 Approved for publto release . p, - _ , r| .. .... . " iinnmrib ....on un.l i td %"n I - _LCLAILIU SECURITY...34Wake Dispersion in Turbomachines." Transac- tions of the ASME, Journal of Basic Engineering, BBD : 688-690. 87 Wagner, J. H., and Okiishi, T. H. 1977

46 CFR 54.20-3 - Design (modifies UW-9, UW-11(a), UW-13, and UW-16).

Code of Federal Regulations, 2010 CFR

2010-10-01

... ENGINEERING PRESSURE VESSELS Fabrication by Welding § 54.20-3 Design (modifies UW-9, UW-11(a), UW-13, and UW-16). (a) Fabrication by welding shall be in accordance with the provisions of this part and with part 57 of this subchapter. (b) Welding subject to UW-11(a) of section VIII of the ASME Boiler and...

Computer Aided Process Planning of Machined Metal Parts

DTIC Science & Technology

1984-09-01

the manufac- turer to accentuate the positive to assist marketing . Machine usage costs and facility loadings are frequently critical. For example...Variant systems currently on the market include Multiplan (TM of OIR, Inc.), CY-Miplan (TM of Computervision), PICAPP (TM of PICAPP, Inc.) and CSD...Multiproduct, Multistage Manufacturing Systems, Journal of Engineering for Industry, ASME, August 1977. Hitomi, K. and I. Ham, Product Mix and Machine Loading

46 CFR 52.25-3 - Feedwater heaters (modifies PFH-1).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Feedwater heaters (modifies PFH-1). 52.25-3 Section 52.25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS Other Boiler Types § 52.25-3 Feedwater heaters (modifies PFH-1). In addition to the requirements in PFH-1 of section I of the ASME Boiler and...

46 CFR 52.25-3 - Feedwater heaters (modifies PFH-1).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Feedwater heaters (modifies PFH-1). 52.25-3 Section 52.25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS Other Boiler Types § 52.25-3 Feedwater heaters (modifies PFH-1). In addition to the requirements in PFH-1 of section I of the ASME Boiler and...

46 CFR 52.25-3 - Feedwater heaters (modifies PFH-1).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Feedwater heaters (modifies PFH-1). 52.25-3 Section 52.25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS Other Boiler Types § 52.25-3 Feedwater heaters (modifies PFH-1). In addition to the requirements in PFH-1 of section I of the ASME Boiler and...

46 CFR 52.25-3 - Feedwater heaters (modifies PFH-1).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Feedwater heaters (modifies PFH-1). 52.25-3 Section 52.25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS Other Boiler Types § 52.25-3 Feedwater heaters (modifies PFH-1). In addition to the requirements in PFH-1 of section I of the ASME Boiler and...

46 CFR 52.25-3 - Feedwater heaters (modifies PFH-1).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Feedwater heaters (modifies PFH-1). 52.25-3 Section 52.25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS Other Boiler Types § 52.25-3 Feedwater heaters (modifies PFH-1). In addition to the requirements in PFH-1 of section I of the ASME Boiler and...

46 CFR 54.20-3 - Design (modifies UW-9, UW-11(a), UW-13, and UW-16).

Code of Federal Regulations, 2012 CFR

2012-10-01

... ENGINEERING PRESSURE VESSELS Fabrication by Welding § 54.20-3 Design (modifies UW-9, UW-11(a), UW-13, and UW-16). (a) Fabrication by welding shall be in accordance with the provisions of this part and with part 57 of this subchapter. (b) Welding subject to UW-11(a) of section VIII of the ASME Boiler and...

46 CFR 54.20-3 - Design (modifies UW-9, UW-11(a), UW-13, and UW-16).

Code of Federal Regulations, 2011 CFR

2011-10-01

... ENGINEERING PRESSURE VESSELS Fabrication by Welding § 54.20-3 Design (modifies UW-9, UW-11(a), UW-13, and UW-16). (a) Fabrication by welding shall be in accordance with the provisions of this part and with part 57 of this subchapter. (b) Welding subject to UW-11(a) of section VIII of the ASME Boiler and...

46 CFR 54.20-3 - Design (modifies UW-9, UW-11(a), UW-13, and UW-16).

Code of Federal Regulations, 2014 CFR

2014-10-01

... ENGINEERING PRESSURE VESSELS Fabrication by Welding § 54.20-3 Design (modifies UW-9, UW-11(a), UW-13, and UW-16). (a) Fabrication by welding shall be in accordance with the provisions of this part and with part 57 of this subchapter. (b) Welding subject to UW-11(a) of section VIII of the ASME Boiler and...

46 CFR 54.20-3 - Design (modifies UW-9, UW-11(a), UW-13, and UW-16).

Code of Federal Regulations, 2013 CFR

2013-10-01

... ENGINEERING PRESSURE VESSELS Fabrication by Welding § 54.20-3 Design (modifies UW-9, UW-11(a), UW-13, and UW-16). (a) Fabrication by welding shall be in accordance with the provisions of this part and with part 57 of this subchapter. (b) Welding subject to UW-11(a) of section VIII of the ASME Boiler and...

University NanoSat Program: AggieSat3

DTIC Science & Technology

2009-06-01

commercially available product for stereo machine vision developed by Point Grey Research. The current binocular BumbleBee2® system incorporates two...and Fellow of the American Society of Mechanical Engineers (ASME) in 1997. She was awarded the 2007 J. Leland "Lee" Atwood Award from the ASEE...AggieSat2 satellite programs. Additional experience gained in the area of drawing standards, machining capabilities, solid modeling, safety

Elevation of Serum Acid Sphingomyelinase Activity in Children with Acute Respiratory Syncytial Virus Bronchiolitis.

PubMed

Yoshida, Shuichiro; Noguchi, Atsuko; Kikuchi, Wataru; Fukaya, Hiroshi; Igarashi, Kiyoshi; Takahashi, Tsutomu

2017-12-01

Acid sphingomyelinase (ASM) is a lysosomal enzyme that hydrolyzes sphingomyelin into ceramide, a bioactive lipid to regulate cellular physiological functions. Thus, ASM activation has been reported as a key event in pathophysiological reactions including inflammation, cytokine release, oxidative stress, and endothelial damage in human diseases. Since ASM activation is associated with extracellular ASM secretion through unknown mechanisms, it can be detected by recognizing the elevation of secretory ASM (S-ASM) activity. Serum S-ASM activity has been reported to increase in chronic diseases, acute cardiac diseases, and systemic inflammatory diseases. However, the serum S-ASM has not been investigated in common acute illness. This study was designed to evaluate serum S-ASM activity in children with common acute illness. Fifty children with common acute illness and five healthy children were included in this study. The patients were categorized into five groups based on clinical diagnoses: acute respiratory syncytial virus (RSV) bronchiolitis, adenovirus infection, streptococcal infection, asthma, and other infections due to unknown origin. The serum S-ASM activity was significantly elevated at 6.9 ± 1.6 nmol/0.1 mL/6 h in the group of acute RSV bronchiolitis patients compared with healthy children who had a mean level of 1.8 ± 0.8 nmol/0.1 mL/6 h (p < 0.05). In the other illness groups, the serum S-ASM activity was not significantly elevated. The results suggest an association of ASM activation with RSV infection, a cause for common acute illness. This is the first report to describe the elevation of serum S-ASM activity in respiratory tract infection.

The contribution of airway smooth muscle to airway narrowing and airway hyperresponsiveness in disease.

PubMed

Martin, J G; Duguet, A; Eidelman, D H

2000-08-01

Airway hyperresponsiveness (AHR), the exaggerated response to constrictor agonists in asthmatic subjects, is incompletely understood. Changes in either the quantity or properties of airway smooth muscle (ASM) are possible explanations for AHR. Morphometric analyses demonstrate structural changes in asthmatic airways, including subepithelial fibrosis, gland hyperplasia/hypertrophy, neovascularization and an increase in ASM mass. Mathematical modelling of airway narrowing suggests that, of all the changes in structure, the increase in ASM mass is the most probable cause of AHR. An increase in ASM mass in the large airways is more closely associated with a greater likelihood of dying from asthma than increases in ASM mass in other locations within the airway tree. ASM contraction is opposed by the elastic recoil of the lungs and airways, which appears to limit the degree of bronchoconstriction in vivo. The cyclical nature of tidal breathing applies stresses to the airway wall that enhance the bronchodilating influence of the lung tissues on the contracting ASM, in all probability by disrupting cross-bridges. However, the increase in ASM mass in asthma may overcome the limitation resulting from the impedances to ASM shortening imposed by the lung parenchyma and airway wall tissues. Additionally, ASM with the capacity to shorten rapidly may achieve shorter lengths and cause a greater degree of bronchoconstriction when stimulated to contract than slower ASM. Changes in ASM properties are induced by the process of sensitization and allergen-exposure such as enhancement of phospholipase C activity and inositol phosphate turnover, and increases in myosin light chain kinase activity. Whether changes in ASM mass or biochemical/biomechanical properties form the basis for asthma remains to be determined.

Light Water Reactor Sustainability Program Grizzly Year-End Progress Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Benjamin Spencer; Yongfeng Zhang; Pritam Chakraborty

2013-09-01

The Grizzly software application is being developed under the Light Water Reactor Sustainability (LWRS) program to address aging and material degradation issues that could potentially become an obstacle to life extension of nuclear power plants beyond 60 years of operation. Grizzly is based on INL’s MOOSE multiphysics simulation environment, and can simultaneously solve a variety of tightly coupled physics equations, and is thus a very powerful and flexible tool with a wide range of potential applications. Grizzly, the development of which was begun during fiscal year (FY) 2012, is intended to address degradation in a variety of critical structures. Themore » reactor pressure vessel (RPV) was chosen for an initial application of this software. Because it fulfills the critical roles of housing the reactor core and providing a barrier to the release of coolant, the RPV is clearly one of the most safety-critical components of a nuclear power plant. In addition, because of its cost, size and location in the plant, replacement of this component would be prohibitively expensive, so failure of the RPV to meet acceptance criteria would likely result in the shutting down of a nuclear power plant. The current practice used to perform engineering evaluations of the susceptibility of RPVs to fracture is to use the ASME Master Fracture Toughness Curve (ASME Code Case N-631 Section III). This is used in conjunction with empirically based models that describe the evolution of this curve due to embrittlement in terms of a transition temperature shift. These models are based on an extensive database of surveillance coupons that have been irradiated in operating nuclear power plants, but this data is limited to the lifetime of the current reactor fleet. This is an important limitation when considering life extension beyond 60 years. The currently available data cannot be extrapolated with confidence further out in time because there is a potential for additional damage mechanisms (i.e. late blooming phases) to become active later in life beyond the current operational experience. To develop a tool that can eventually serve a role in decision-making, it is clear that research and development must be perfomed at multiple scales. At the engineering scale, a multiphysics analysis code that can capture the thermomechanical response of the RPV under accident conditions, including detailed fracture mechanics evaluations of flaws with arbitrary geometry and orientation, is needed to assess whether the fracture toughness, as defined by the master curve, including the effects of embrittlement, is exceeded. At the atomistic scale, the fundamental mechanisms of degradation need to be understood, including the effects of that degradation on the relevant material properties. In addition, there is a need to better understand the mechanisms leading to the transition from ductile to brittle fracture through improved continuum mechanics modeling at the fracture coupon scale. Work is currently being conducted at all of these levels with the goal of creating a usable engineering tool informed by lower length-scale modeling. This report summarizes progress made in these efforts during FY 2013.« less

Predictors and Long-Term Clinical Impact of Acute Stent Malapposition: An Assessment of Dual Antiplatelet Therapy With Drug-Eluting Stents (ADAPT-DES) Intravascular Ultrasound Substudy.

PubMed

Wang, Bin; Mintz, Gary S; Witzenbichler, Bernhard; Souza, Cristiano F; Metzger, D Christopher; Rinaldi, Michael J; Duffy, Peter L; Weisz, Giora; Stuckey, Thomas D; Brodie, Bruce R; Matsumura, Mitsuaki; Yamamoto, Myong-Hwa; Parvataneni, Rupa; Kirtane, Ajay J; Stone, Gregg W; Maehara, Akiko

2016-12-22

The impact of acute stent malapposition (ASM) on long-term clinical outcomes in patients undergoing percutaneous coronary intervention is still controversial. We sought to evaluate predictors and long-term clinical outcomes of ASM. ADAPT-DES (Assessment of Dual Antiplatelet Therapy With Drug-Eluting Stents) was a prospective multicenter study of 8663 patients undergoing percutaneous coronary intervention using drug-eluting stents. In a prespecified intravascular ultrasound-guided substudy, 2072 patients with 2446 culprit lesions had post-percutaneous coronary intervention intravascular ultrasound and were classified according to the presence or absence of ASM. After intravascular ultrasound-guided percutaneous coronary intervention, the overall prevalence of ASM after successful drug-eluting stents implantation was 14.4% per patient and 12.6% per lesion. Compared to lesions without ASM, lesions with ASM had larger in-stent lumen areas, larger stent areas, and larger in-stent vessel areas. A larger mean plaque area along with more attenuated plaque was observed in lesions with ASM versus lesions without ASM. Lesions with ASM had greater proximal and distal reference lumen areas and more distal, but not proximal, reference calcium compared to lesions without ASM. At 2-year follow-up, there was no significant difference in the incidence of cardiac death; myocardial infarction; early, late, or very late stent thrombosis; or clinically driven target lesion revascularization in patients with ASM versus those without ASM. Furthermore, ASM was not an independent predictor of 2-year major adverse cardiac events or target lesion revascularization even when forced into the multivariate model. In patients treated with intravascular ultrasound-guided drug-eluting stents implantation, ASM was not associated with adverse clinical events during long-term follow-up including, but not limited to, stent thrombosis. URL: https://www.clinicaltrials.gov. Unique identifier: NCT00638794. © 2016 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

ASM-3 Acid Sphingomyelinase Functions as a Positive Regulator of the DAF-2/AGE-1 Signaling Pathway and Serves as a Novel Anti-Aging Target

PubMed Central

Kim, Yongsoon; Sun, Hong

2012-01-01

In C. elegans, the highly conserved DAF-2/insulin/insulin-like growth factor 1 receptor signaling (IIS) pathway regulates longevity, metabolism, reproduction and development. In mammals, acid sphingomyelinase (ASM) is an enzyme that hydrolyzes sphingomyelin to produce ceramide. ASM has been implicated in CD95 death receptor signaling under certain stress conditions. However, the involvement of ASM in growth factor receptor signaling under physiological conditions is not known. Here, we report that in vivo ASM functions as a positive regulator of the DAF-2/IIS pathway in C. elegans. We have shown that inactivation of asm-3 extends animal lifespan and promotes dauer arrest, an alternative developmental process. A significant cooperative effect on lifespan is observed between asm-3 deficiency and loss-of-function alleles of the age-1/PI 3-kinase, with the asm-3; age-1 double mutant animals having a mean lifespan 259% greater than that of the wild-type animals. The lifespan extension phenotypes caused by the loss of asm-3 are dependent on the functions of daf-16/FOXO and daf-18/PTEN. We have demonstrated that inactivation of asm-3 causes nuclear translocation of DAF-16::GFP protein, up-regulates endogenous DAF-16 protein levels and activates the downstream targeting genes of DAF-16. Together, our findings reveal a novel role of asm-3 in regulation of lifespan and diapause by modulating IIS pathway. Importantly, we have found that two drugs known to inhibit mammalian ASM activities, desipramine and clomipramine, markedly extend the lifespan of wild-type animals, in a manner similar to that achieved by genetic inactivation of the asm genes. Our studies illustrate a novel strategy of anti-aging by targeting ASM, which may potentially be extended to mammals. PMID:23049887

GC-ASM: Synergistic Integration of Graph-Cut and Active Shape Model Strategies for Medical Image Segmentation

PubMed Central

Chen, Xinjian; Udupa, Jayaram K.; Alavi, Abass; Torigian, Drew A.

2013-01-01

Image segmentation methods may be classified into two categories: purely image based and model based. Each of these two classes has its own advantages and disadvantages. In this paper, we propose a novel synergistic combination of the image based graph-cut (GC) method with the model based ASM method to arrive at the GC-ASM method for medical image segmentation. A multi-object GC cost function is proposed which effectively integrates the ASM shape information into the GC framework. The proposed method consists of two phases: model building and segmentation. In the model building phase, the ASM model is built and the parameters of the GC are estimated. The segmentation phase consists of two main steps: initialization (recognition) and delineation. For initialization, an automatic method is proposed which estimates the pose (translation, orientation, and scale) of the model, and obtains a rough segmentation result which also provides the shape information for the GC method. For delineation, an iterative GC-ASM algorithm is proposed which performs finer delineation based on the initialization results. The proposed methods are implemented to operate on 2D images and evaluated on clinical chest CT, abdominal CT, and foot MRI data sets. The results show the following: (a) An overall delineation accuracy of TPVF > 96%, FPVF < 0.6% can be achieved via GC-ASM for different objects, modalities, and body regions. (b) GC-ASM improves over ASM in its accuracy and precision to search region. (c) GC-ASM requires far fewer landmarks (about 1/3 of ASM) than ASM. (d) GC-ASM achieves full automation in the segmentation step compared to GC which requires seed specification and improves on the accuracy of GC. (e) One disadvantage of GC-ASM is its increased computational expense owing to the iterative nature of the algorithm. PMID:23585712

ASM-3 acid sphingomyelinase functions as a positive regulator of the DAF-2/AGE-1 signaling pathway and serves as a novel anti-aging target.

PubMed

Kim, Yongsoon; Sun, Hong

2012-01-01

In C. elegans, the highly conserved DAF-2/insulin/insulin-like growth factor 1 receptor signaling (IIS) pathway regulates longevity, metabolism, reproduction and development. In mammals, acid sphingomyelinase (ASM) is an enzyme that hydrolyzes sphingomyelin to produce ceramide. ASM has been implicated in CD95 death receptor signaling under certain stress conditions. However, the involvement of ASM in growth factor receptor signaling under physiological conditions is not known. Here, we report that in vivo ASM functions as a positive regulator of the DAF-2/IIS pathway in C. elegans. We have shown that inactivation of asm-3 extends animal lifespan and promotes dauer arrest, an alternative developmental process. A significant cooperative effect on lifespan is observed between asm-3 deficiency and loss-of-function alleles of the age-1/PI 3-kinase, with the asm-3; age-1 double mutant animals having a mean lifespan 259% greater than that of the wild-type animals. The lifespan extension phenotypes caused by the loss of asm-3 are dependent on the functions of daf-16/FOXO and daf-18/PTEN. We have demonstrated that inactivation of asm-3 causes nuclear translocation of DAF-16::GFP protein, up-regulates endogenous DAF-16 protein levels and activates the downstream targeting genes of DAF-16. Together, our findings reveal a novel role of asm-3 in regulation of lifespan and diapause by modulating IIS pathway. Importantly, we have found that two drugs known to inhibit mammalian ASM activities, desipramine and clomipramine, markedly extend the lifespan of wild-type animals, in a manner similar to that achieved by genetic inactivation of the asm genes. Our studies illustrate a novel strategy of anti-aging by targeting ASM, which may potentially be extended to mammals.

Suppression of Eosinophil Integrins Prevents Remodeling of Airway Smooth Muscle in Asthma

PubMed Central

Januskevicius, Andrius; Gosens, Reinoud; Sakalauskas, Raimundas; Vaitkiene, Simona; Janulaityte, Ieva; Halayko, Andrew J.; Hoppenot, Deimante; Malakauskas, Kestutis

2017-01-01

Background: Airway smooth muscle (ASM) remodeling is an important component of the structural changes to airways seen in asthma. Eosinophils are the prominent inflammatory cells in asthma, and there is some evidence that they contribute to ASM remodeling via released mediators and direct contact through integrin–ligand interactions. Eosinophils express several types of outer membrane integrin, which are responsible for cell–cell and cell–extracellular matrix interactions. In our previous study we demonstrated that asthmatic eosinophils show increased adhesion to ASM cells and it may be important factor contributing to ASM remodeling in asthma. According to these findings, in the present study we investigated the effects of suppression of eosinophil integrin on eosinophil-induced ASM remodeling in asthma. Materials and Methods: Individual combined cell cultures of immortalized human ASM cells and eosinophils from peripheral blood of 22 asthmatic patients and 17 healthy controls were prepared. Eosinophil adhesion was evaluated using eosinophil peroxidase activity assay. Genes expression levels in ASM cells and eosinophils were measured using quantitative real-time PCR. ASM cell proliferation was measured using alamarBlue® solution. Eosinophil integrins were blocked by incubating with Arg-Gly-Asp-Ser peptide. Results: Eosinophils from the asthma group showed increased outer membrane α4β1 and αMβ2 integrin expression, increased adhesion to ASM cells, and overexpression of TGF-β1 compared with eosinophils from the healthy control group. Blockade of eosinophil RGD-binding integrins by Arg-Gly-Asp-Ser peptide significantly reduced adhesion of eosinophils to ASM cells in both groups. Integrin-blocking decreased the effects of eosinophils on TGF-β1, WNT-5a, and extracellular matrix protein gene expression in ASM cells and ASM cell proliferation in both groups. These effects were more pronounced in the asthma group compared with the control group. Conclusion: Suppression of eosinophil-ASM interaction via RGD-binding integrins attenuates eosinophil-induced ASM remodeling in asthma. Trial Registration: ClinicalTrials.gov Identifier: NCT02648074. PMID:28119625

GC-ASM: Synergistic Integration of Graph-Cut and Active Shape Model Strategies for Medical Image Segmentation.

PubMed

Chen, Xinjian; Udupa, Jayaram K; Alavi, Abass; Torigian, Drew A

2013-05-01

Image segmentation methods may be classified into two categories: purely image based and model based. Each of these two classes has its own advantages and disadvantages. In this paper, we propose a novel synergistic combination of the image based graph-cut (GC) method with the model based ASM method to arrive at the GC-ASM method for medical image segmentation. A multi-object GC cost function is proposed which effectively integrates the ASM shape information into the GC framework. The proposed method consists of two phases: model building and segmentation. In the model building phase, the ASM model is built and the parameters of the GC are estimated. The segmentation phase consists of two main steps: initialization (recognition) and delineation. For initialization, an automatic method is proposed which estimates the pose (translation, orientation, and scale) of the model, and obtains a rough segmentation result which also provides the shape information for the GC method. For delineation, an iterative GC-ASM algorithm is proposed which performs finer delineation based on the initialization results. The proposed methods are implemented to operate on 2D images and evaluated on clinical chest CT, abdominal CT, and foot MRI data sets. The results show the following: (a) An overall delineation accuracy of TPVF > 96%, FPVF < 0.6% can be achieved via GC-ASM for different objects, modalities, and body regions. (b) GC-ASM improves over ASM in its accuracy and precision to search region. (c) GC-ASM requires far fewer landmarks (about 1/3 of ASM) than ASM. (d) GC-ASM achieves full automation in the segmentation step compared to GC which requires seed specification and improves on the accuracy of GC. (e) One disadvantage of GC-ASM is its increased computational expense owing to the iterative nature of the algorithm.

Risk of cardiac events in Long QT syndrome patients when taking antiseizure medications.

PubMed

Auerbach, David S; Biton, Yitschak; Polonsky, Bronislava; McNitt, Scott; Gross, Robert A; Dirksen, Robert T; Moss, Arthur J

2018-01-01

Many antiseizure medications (ASMs) affect ion channel function. We investigated whether ASMs alter the risk of cardiac events in patients with corrected QT (QT c ) prolongation. The study included people from the Rochester-based Long QT syndrome (LQTS) Registry with baseline QT c prolongation and history of ASM therapy (n = 296). Using multivariate Anderson-Gill models, we assessed the risk of recurrent cardiac events associated with ASM therapy. We stratified by LQTS genotype and predominant mechanism of ASM action (Na + channel blocker and gamma-aminobutyric acid modifier.) There was an increased risk of cardiac events when participants with QT c prolongation were taking vs off ASMs (HR 1.65, 95% confidence interval [CI] 1.36-2.00, P < 0.001). There was an increased risk of cardiac events when LQTS2 (HR 1.49, 95% CI 1.03-2.15, P = 0.036) but not LQTS1 participants were taking ASMs (interaction, P = 0.016). Na + channel blocker ASMs were associated with an increased risk of cardiac events in participants with QT c prolongation, specifically LQTS2, but decreased risk in LQTS1. The increased risk when taking all ASMs and Na + channel blocker ASMs was attenuated by concurrent beta-adrenergic blocker therapy (interaction, P < 0.001). Gamma-aminobutyric acid modifier ASMs were associated with an increased risk of events in patients not concurrently treated with beta-adrenergic blockers. Female participants were at an increased risk of cardiac events while taking all ASMs and each class of ASMs. Despite no change in overall QT c duration, pharmacogenomic analyses set the stage for future prospective clinical and mechanistic studies to validate that ASMs with predominantly Na + channel blocking actions are deleterious in LQTS2, but protective in LQTS1. Copyright © 2017 Elsevier Inc. All rights reserved.

The ASM-NSF Biology Scholars Program: An Evidence-Based Model for Faculty Development.

PubMed

Chang, Amy L; Pribbenow, Christine M

2016-05-01

The American Society for Microbiology (ASM) established its ASM-NSF (National Science Foundation) Biology Scholars Program (BSP) to promote undergraduate education reform by 1) supporting biologists to implement evidence-based teaching practices, 2) engaging life science professional societies to facilitate biologists' leadership in scholarly teaching within the discipline, and 3) participating in a teaching community that fosters disciplinary-level science, technology, engineering, and mathematics (STEM) reform. Since 2005, the program has utilized year-long residency training to provide a continuum of learning and practice centered on principles from the scholarship of teaching and learning (SoTL) to more than 270 participants ("scholars") from biology and multiple other disciplines. Additionally, the program has recruited 11 life science professional societies to support faculty development in SoTL and discipline-based education research (DBER). To identify the BSP's long-term outcomes and impacts, ASM engaged an external evaluator to conduct a study of the program's 2010-2014 scholars (n = 127) and society partners. The study methods included online surveys, focus groups, participant observation, and analysis of various documents. Study participants indicate that the program achieved its proposed goals relative to scholarship, professional society impact, leadership, community, and faculty professional development. Although participants also identified barriers that hindered elements of their BSP participation, findings suggest that the program was essential to their development as faculty and provides evidence of the BSP as a model for other societies seeking to advance undergraduate science education reform. The BSP is the longest-standing faculty development program sponsored by a collective group of life science societies. This collaboration promotes success across a fragmented system of more than 80 societies representing the life sciences and helps catalyze biology education reform efforts.

The ASM-NSF Biology Scholars Program: An Evidence-Based Model for Faculty Development

PubMed Central

Chang, Amy L.; Pribbenow, Christine M.

2016-01-01

The American Society for Microbiology (ASM) established its ASM-NSF (National Science Foundation) Biology Scholars Program (BSP) to promote undergraduate education reform by 1) supporting biologists to implement evidence-based teaching practices, 2) engaging life science professional societies to facilitate biologists’ leadership in scholarly teaching within the discipline, and 3) participating in a teaching community that fosters disciplinary-level science, technology, engineering, and mathematics (STEM) reform. Since 2005, the program has utilized year-long residency training to provide a continuum of learning and practice centered on principles from the scholarship of teaching and learning (SoTL) to more than 270 participants (“scholars”) from biology and multiple other disciplines. Additionally, the program has recruited 11 life science professional societies to support faculty development in SoTL and discipline-based education research (DBER). To identify the BSP’s long-term outcomes and impacts, ASM engaged an external evaluator to conduct a study of the program’s 2010–2014 scholars (n = 127) and society partners. The study methods included online surveys, focus groups, participant observation, and analysis of various documents. Study participants indicate that the program achieved its proposed goals relative to scholarship, professional society impact, leadership, community, and faculty professional development. Although participants also identified barriers that hindered elements of their BSP participation, findings suggest that the program was essential to their development as faculty and provides evidence of the BSP as a model for other societies seeking to advance undergraduate science education reform. The BSP is the longest-standing faculty development program sponsored by a collective group of life science societies. This collaboration promotes success across a fragmented system of more than 80 societies representing the life sciences and helps catalyze biology education reform efforts. PMID:27158300

A Macro Analysis of DoD Logistics Systems. Volume 2. Structure and Analysis of the Air Force Logistics System

DTIC Science & Technology

1977-09-01

performance measures discussed earlier is the "Engine Actuarial Data Summary" (EADS) (AFLC Form 992), compiled from D024F actuarial data. EADS is...3.2,452.3 PlyLWn M"Is, 404.670 432.603 408,553. 413.940 PMiSMA-00023 AF asm -haiw pua-flywa hma tread =100% PTtU94411 "Weul ’A101) 16,839.6 1.6,308.0...Engine Actuarial Data Summary. ENORS - 2ngine Not Operationally Ready, Supply. EOQ Items - Economic Order Quantity Items; i.e., expense-type items, not

Correlation Study of Laboratory Physical and Chemical Data with Dynamometer Engine Sequence Performance Testing of Engine Lubricating Oils.

DTIC Science & Technology

1978-12-01

and chemical data and dynamomet er and field perfo rmance. The initial stud y produced internal and group correlations among the data and suggests a...sm all (a corre lation of I .0 i~ ~~r kct cor rela t ion ’, Prin cip al comp onent .iii,il ~ 515 Was enmp lo~ ed to stud ~ lhe iner al l re hi t i...Additive ) K = Potassium (Addit ive) V N = Nitr ogen (Additive ) . V Mg = Magnesium (Additive ) B = Boron (Additive ) Other = To include wear and

The ASM Curriculum Guidelines for Undergraduate Microbiology: A Case Study of the Advocacy Role of Societies in Reform Efforts

PubMed Central

Horak, Rachel E. A.; Merkel, Susan; Chang, Amy

2015-01-01

A number of national reports, including Vision and Change in Undergraduate Biology Education: A Call to Action, have called for drastic changes in how undergraduate biology is taught. To that end, the American Society for Microbiology (ASM) has developed new Curriculum Guidelines for undergraduate microbiology that outline a comprehensive curriculum for any undergraduate introductory microbiology course or program of study. Designed to foster enduring understanding of core microbiology concepts, the Guidelines work synergistically with backwards course design to focus teaching on student-centered goals and priorities. In order to qualitatively assess how the ASM Curriculum Guidelines are used by educators and learn more about the needs of microbiology educators, the ASM Education Board distributed two surveys to the ASM education community. In this report, we discuss the results of these surveys (353 responses). We found that the ASM Curriculum Guidelines are being implemented in many different types of courses at all undergraduate levels. Educators indicated that the ASM Curriculum Guidelines were very helpful when planning courses and assessments. We discuss some specific ways in which the ASM Curriculum Guidelines have been used in undergraduate classrooms. The survey identified some barriers that microbiology educators faced when trying to adopt the ASM Curriculum Guidelines, including lack of time, lack of financial resources, and lack of supporting resources. Given the self-reported challenges to implementing the ASM Curriculum Guidelines in undergraduate classrooms, we identify here some activities related to the ASM Curriculum Guidelines that the ASM Education Board has initiated to assist educators in the implementation process. PMID:25949769

The ASM Curriculum Guidelines for Undergraduate Microbiology: A Case Study of the Advocacy Role of Societies in Reform Efforts.

PubMed

Horak, Rachel E A; Merkel, Susan; Chang, Amy

2015-05-01

A number of national reports, including Vision and Change in Undergraduate Biology Education: A Call to Action, have called for drastic changes in how undergraduate biology is taught. To that end, the American Society for Microbiology (ASM) has developed new Curriculum Guidelines for undergraduate microbiology that outline a comprehensive curriculum for any undergraduate introductory microbiology course or program of study. Designed to foster enduring understanding of core microbiology concepts, the Guidelines work synergistically with backwards course design to focus teaching on student-centered goals and priorities. In order to qualitatively assess how the ASM Curriculum Guidelines are used by educators and learn more about the needs of microbiology educators, the ASM Education Board distributed two surveys to the ASM education community. In this report, we discuss the results of these surveys (353 responses). We found that the ASM Curriculum Guidelines are being implemented in many different types of courses at all undergraduate levels. Educators indicated that the ASM Curriculum Guidelines were very helpful when planning courses and assessments. We discuss some specific ways in which the ASM Curriculum Guidelines have been used in undergraduate classrooms. The survey identified some barriers that microbiology educators faced when trying to adopt the ASM Curriculum Guidelines, including lack of time, lack of financial resources, and lack of supporting resources. Given the self-reported challenges to implementing the ASM Curriculum Guidelines in undergraduate classrooms, we identify here some activities related to the ASM Curriculum Guidelines that the ASM Education Board has initiated to assist educators in the implementation process.

46 CFR 56.60-1 - Acceptable materials and specifications (replaces 123 and Table 126.1 in ASME B31.1).

Code of Federal Regulations, 2014 CFR

2014-10-01

... standards ASME standards Notes Pipe, seamless: A 106 Carbon steel ASME B31.1 A 335 Ferritic alloys ASME B31.1 A 376 Austenitic alloys ASME B31.1 (1). Pipe, seamless and welded: A 53 Types S, F, and E steel... cast: (None applicable) (1 9) Tube, seamless: A 179 Carbon steel heat exchanger and condenser tubes...

46 CFR 56.60-1 - Acceptable materials and specifications (replaces 123 and Table 126.1 in ASME B31.1).

Code of Federal Regulations, 2013 CFR

2013-10-01

... standards ASME standards Notes Pipe, seamless: A 106 Carbon steel ASME B31.1 A 335 Ferritic alloys ASME B31.1 A 376 Austenitic alloys ASME B31.1 (1). Pipe, seamless and welded: A 53 Types S, F, and E steel... cast: (None applicable) (1 9) Tube, seamless: A 179 Carbon steel heat exchanger and condenser tubes...

46 CFR 56.60-1 - Acceptable materials and specifications (replaces 123 and Table 126.1 in ASME B31.1).

Code of Federal Regulations, 2012 CFR

2012-10-01

... standards ASME standards Notes Pipe, seamless: A 106 Carbon steel ASME B31.1 A 335 Ferritic alloys ASME B31.1 A 376 Austenitic alloys ASME B31.1 (1). Pipe, seamless and welded: A 53 Types S, F, and E steel... cast: (None applicable) (1,9) Tube, seamless: A 179 Carbon steel heat exchanger and condenser tubes...

Airway smooth muscle contraction - perspectives on past, present and future.

PubMed

Mitchell, H W

2009-10-01

Past and contemporary views of airway smooth muscle (ASM) have led to a high level of understanding of the control and intracellular regulation of force or shortening of ASM and of its possible role in airway disease. As well as the multitude of cellular mechanisms that regulate ASM contraction, a number of structural and mechanical factors, which are only present at the airway and lung level, provide overriding control over ASM. With new knowledge about the cellular physiology and biology of ASM, there is increasing need to understand how ASM contraction is regulated and expressed at these airway and system levels.

Nonlinear Dynamic Analysis of Disordered Bladed-Disk Assemblies

NASA Technical Reports Server (NTRS)

McGee, Oliver G., III

1997-01-01

In a effort to address current needs for efficient, air propulsion systems, we have developed some new analytical predictive tools for understanding and alleviating aircraft engine instabilities which have led to accelerated high cycle fatigue and catastrophic failures of these machines during flight. A frequent cause of failure in Jets engines is excessive resonant vibrations and stall flutter instabilities. The likelihood of these phenomena is reduced when designers employ the analytical models we have developed. These prediction models will ultimately increase the nation's competitiveness in producing high performance Jets engines with enhanced operability, energy economy, and safety. The objectives of our current threads of research in the final year are directed along two lines. First, we want to improve the current state of blade stress and aeromechanical reduced-ordered modeling of high bypass engine fans, Specifically, a new reduced-order iterative redesign tool for passively controlling the mechanical authority of shroudless, wide chord, laminated composite transonic bypass engine fans has been developed. Second, we aim to advance current understanding of aeromechanical feedback control of dynamic flow instabilities in axial flow compressors. A systematic theoretical evaluation of several approaches to aeromechanical feedback control of rotating stall in axial compressors has been conducted. Attached are abstracts of two .papers under preparation for the 1998 ASME Turbo Expo in Stockholm, Sweden sponsored under Grant No. NAG3-1571. Our goals during the final year under Grant No. NAG3-1571 is to enhance NASA's capabilities of forced response of turbomachines (such as NASA FREPS). We with continue our development of the reduced-ordered, three-dimensional component synthesis models for aeromechanical evaluation of integrated bladeddisk assemblies (i.e., the disk, non-identical bladeing etc.). We will complete our development of component systems design optimization strategies for specified vibratory stresses and increased fatigue life prediction of assembly components, and for specified frequency margins on the Campbell diagrams of turbomachines. Finally, we will integrate the developed codes with NASA's turbomachinery aeromechanics prediction capability (such as NASA FREPS).

The Unconventional Role of Acid Sphingomyelinase in Regulation of Retinal Microangiopathy in Diabetic Human and Animal Models

PubMed Central

Opreanu, Madalina; Tikhonenko, Maria; Bozack, Svetlana; Lydic, Todd A.; Reid, Gavin E.; McSorley, Kelly M.; Sochacki, Andrew; Perez, Gloria I.; Esselman, Walter J.; Kern, Timothy; Kolesnick, Richard; Grant, Maria B.; Busik, Julia V.

2011-01-01

OBJECTIVE Acid sphingomyelinase (ASM) is an important early responder in inflammatory cytokine signaling. The role of ASM in retinal vascular inflammation and vessel loss associated with diabetic retinopathy is not known and represents the goal of this study. RESEARCH DESIGN AND METHODS Protein and gene expression profiles were determined by quantitative RT-PCR and Western blot. ASM activity was determined using Amplex Red sphingomyelinase assay. Caveolar lipid composition was analyzed by nano-electrospray ionization tandem mass spectrometry. Streptozotocin-induced diabetes and retinal ischemia-reperfusion models were used in in vivo studies. RESULTS We identify endothelial caveolae-associated ASM as an essential component in mediating inflammation and vascular pathology in in vivo and in vitro models of diabetic retinopathy. Human retinal endothelial cells (HREC), in contrast with glial and epithelial cells, express the plasma membrane form of ASM that overlaps with caveolin-1. Treatment of HREC with docosahexaenoic acid (DHA) specifically reduces expression of the caveolae-associated ASM, prevents a tumor necrosis factor-α–induced increase in the ceramide-to-sphingomyelin ratio in the caveolae, and inhibits cytokine-induced inflammatory signaling. ASM is expressed in both vascular and neuroretina; however, only vascular ASM is specifically increased in the retinas of animal models at the vasodegenerative phase of diabetic retinopathy. The absence of ASM in ASM−/− mice or inhibition of ASM activity by DHA prevents acellular capillary formation. CONCLUSIONS This is the first study demonstrating activation of ASM in the retinal vasculature of diabetic retinopathy animal models. Inhibition of ASM could be further explored as a potential therapeutic strategy in treating diabetic retinopathy. PMID:21771974

Results from Evaluation of Representative ASME AG-1 Section FK Radial Flow Dimple Pleated HEPA Filters Under Elevated Conditions - 12002

DOE Office of Scientific and Technical Information (OSTI.GOV)

Giffin, Paxton K.; Parsons, Michael S.; Rickert, Jaime G.

The American Society of Mechanical Engineers (ASME) has recently added Section FK establishing requirements for radial flow HEPA filters to the Code on Nuclear Air and Gas Treatment (AG-1). Section FK filters are expected to be a major element in the HEPA filtration systems across the US Department of Energy (DOE) complex. Radial flow filters have been used in Europe for some time, however a limited amount of performance evaluation data exists with respect to these new AG-1 Section FK units. In consultation with a technical working group, the Institute for Clean Energy Technology (ICET) at Mississippi State University (MSU)hasmore » evaluated a series of representative AG-1 Section FK dimple pleated radial flow HEPA filters. The effects of elevated relative humidity and temperature conditions on these filters are particularly concerning. Results from the evaluation of Section FK filters under ambient conditions have been presented at the 2011 waste management conference. Additions to the previous test stand to enable high temperature and high humidity testing, a review of the equipment used, the steps taken to characterize the new additions, and the filter test results are presented in this study. Test filters were evaluated at a volumetric flow rate of 56.6 m{sup 3}/min (2000 cfm) and were challenged under ambient conditions with Alumina, Al(OH){sub 3}, until reaching a differential pressure of 1 kPa (4 in. w.c.), at which time the filters were tested, unchallenged with aerosol, at 54 deg. C (130 deg. F) for approximately 1 hour. At the end of that hour water was sprayed near the heat source to maximize vaporization exposing the filter to an elevated relative humidity up to 95%. Collected data include differential pressure, temperature, relative humidity, and volumetric flow rate versus time. (authors)« less

ASM-024, a Piperazinium Compound, Promotes the In Vitro Relaxation of β2-Adrenoreceptor Desensitized Tracheas

PubMed Central

Israël-Assayag, Evelyne; Beaulieu, Marie-Josée; Cormier, Yvon

2015-01-01

Inhaled β2-adrenoreceptor agonists are widely used in asthma and chronic obstructive pulmonary disease (COPD) for bronchoconstriction relief. β2-adrenoreceptor agonists relax airway smooth muscle cells via cyclic adenosine monophosphate (cAMP) mediated pathways. However, prolonged stimulation induces functional desensitization of the β2-adrenoreceptors (β2-AR), potentially leading to reduced clinical efficacy with chronic or prolonged administration. ASM-024, a small synthetic molecule in clinical stage development, has shown activity at the level of nicotinic receptors and possibly at the muscarinic level and presents anti-inflammatory and bronchodilator properties. Aerosolized ASM-024 reduces airway resistance in mice and promotes in-vitro relaxation of tracheal and bronchial preparations from animal and human tissues. ASM-024 increased in vitro relaxation response to maximally effective concentration of short—acting beta-2 agonists in dog and human bronchi. Although the precise mechanisms by which ASM-024 promotes airway smooth muscle (ASM) relaxation remain unclear, we hypothesized that ASM-024 will attenuate and/or abrogate agonist-induced contraction and remain effective despite β2-AR tachyphylaxis. β2-AR tachyphylaxis was induced with salbutamol, salmeterol and formoterol on guinea pig tracheas. The addition of ASM-024 relaxed concentration-dependently intact or β2-AR desensitized tracheal rings precontracted with methacholine. ASM-024 did not induce any elevation of intracellular cAMP in isolated smooth muscle cells; moreover, blockade of the cAMP pathway with an adenylate cyclase inhibitor had no significant effect on ASM-024-induced guinea pig trachea relaxation. Collectively, these findings show that ASM-024 elicits relaxation of β2-AR desensitized tracheal preparations and suggest that ASM-024 mediates smooth muscle relaxation through a different target and signaling pathway than β2-adrenergic receptor agonists. These findings suggest ASM-024 could potentially provide clinical benefit when used adjunctively with inhaled β2-adrenoreceptor agonists in those patients exhibiting a reduced response to their chronic use. PMID:25799096

ASM-024, a piperazinium compound, promotes the in vitro relaxation of β2-adrenoreceptor desensitized tracheas.

PubMed

Israël-Assayag, Evelyne; Beaulieu, Marie-Josée; Cormier, Yvon

2015-01-01

Inhaled β2-adrenoreceptor agonists are widely used in asthma and chronic obstructive pulmonary disease (COPD) for bronchoconstriction relief. β2-Adrenoreceptor agonists relax airway smooth muscle cells via cyclic adenosine monophosphate (cAMP) mediated pathways. However, prolonged stimulation induces functional desensitization of the β2-adrenoreceptors (β2-AR), potentially leading to reduced clinical efficacy with chronic or prolonged administration. ASM-024, a small synthetic molecule in clinical stage development, has shown activity at the level of nicotinic receptors and possibly at the muscarinic level and presents anti-inflammatory and bronchodilator properties. Aerosolized ASM-024 reduces airway resistance in mice and promotes in-vitro relaxation of tracheal and bronchial preparations from animal and human tissues. ASM-024 increased in vitro relaxation response to maximally effective concentration of short-acting beta-2 agonists in dog and human bronchi. Although the precise mechanisms by which ASM-024 promotes airway smooth muscle (ASM) relaxation remain unclear, we hypothesized that ASM-024 will attenuate and/or abrogate agonist-induced contraction and remain effective despite β2-AR tachyphylaxis. β2-AR tachyphylaxis was induced with salbutamol, salmeterol and formoterol on guinea pig tracheas. The addition of ASM-024 relaxed concentration-dependently intact or β2-AR desensitized tracheal rings precontracted with methacholine. ASM-024 did not induce any elevation of intracellular cAMP in isolated smooth muscle cells; moreover, blockade of the cAMP pathway with an adenylate cyclase inhibitor had no significant effect on ASM-024-induced guinea pig trachea relaxation. Collectively, these findings show that ASM-024 elicits relaxation of β2-AR desensitized tracheal preparations and suggest that ASM-024 mediates smooth muscle relaxation through a different target and signaling pathway than β2-adrenergic receptor agonists. These findings suggest ASM-024 could potentially provide clinical benefit when used adjunctively with inhaled β2-adrenoreceptor agonists in those patients exhibiting a reduced response to their chronic use.

Carbon Nanotubes: Miracle of Materials Science?

NASA Technical Reports Server (NTRS)

Files, Bradley S.; Mayeaux, Brian M.

1999-01-01

Article to be sent to Advanced Materials and Processes, journal of ASM International, as attached. This is a news-type technical journal for a large organization of scientists, engineers, salesmen, and managers. The article is quite general, meant to be an introduction to the properties of nanotubes. This is a materials science organization, therefore the article is geared toward using nanotubes for materials uses. Pictures have not been included in this version.

Mechanisms of Smoke Reduction in the High Pressure Combustion of Emulsified Fuels. Volumn I. Construction of Apparatus and Preliminary Experiments.

DTIC Science & Technology

1981-10-01

Naegeli and Thomas Ryan have made a number of helpful suggestions. Ms. D. Miller, Ms. E. Gonzales, and Mr. J. Pryor are thanked for helping prepare this...October 1976. 2. Naegeli , D.W., Fodor, G.E., and Moses, C.A., "Fuel Microemulsions for Jet Engine Smoke Reduction," to be presented at the ASME Gas

MODEL 9977 B(M)F-96 SAFETY ANALYSIS REPORT FOR PACKAGING

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abramczyk, G; Paul Blanton, P; Kurt Eberl, K

2006-05-18

This Safety Analysis Report for Packaging (SARP) documents the analysis and testing performed on and for the 9977 Shipping Package, referred to as the General Purpose Fissile Package (GPFP). The performance evaluation presented in this SARP documents the compliance of the 9977 package with the regulatory safety requirements for Type B packages. Per 10 CFR 71.59, for the 9977 packages evaluated in this SARP, the value of ''N'' is 50, and the Transport Index based on nuclear criticality control is 1.0. The 9977 package is designed with a high degree of single containment. The 9977 complies with 10 CFR 71more » (2002), Department of Energy (DOE) Order 460.1B, DOE Order 460.2, and 10 CFR 20 (2003) for As Low As Reasonably Achievable (ALARA) principles. The 9977 also satisfies the requirements of the Regulations for the Safe Transport of Radioactive Material--1996 Edition (Revised)--Requirements. IAEA Safety Standards, Safety Series No. TS-R-1 (ST-1, Rev.), International Atomic Energy Agency, Vienna, Austria (2000). The 9977 package is designed, analyzed and fabricated in accordance with Section III of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel (B&PV) Code, 1992 edition.« less

The anti-proliferative and anti-inflammatory response of COPD airway smooth muscle cells to hydrogen sulfide.

PubMed

Perry, Mark M; Tildy, Bernadett; Papi, Alberto; Casolari, Paolo; Caramori, Gaetano; Rempel, Karen Limbert; Halayko, Andrew J; Adcock, Ian; Chung, Kian Fan

2018-05-09

COPD is a common, highly debilitating disease of the airways, primarily caused by smoking. Chronic inflammation and structural remodelling are key pathological features of this disease caused, in part, by the aberrant function of airway smooth muscle (ASM). We have previously demonstrated that hydrogen sulfide (H 2 S) can inhibit ASM cell proliferation and CXCL8 release, from cells isolated from non-smokers. We examined the effect of H 2 S upon ASM cells from COPD patients. ASM cells were isolated from non-smokers, smokers and patients with COPD (n = 9). Proliferation and cytokine release (IL-6 and CXCL8) of ASM was induced by FCS, and measured by bromodeoxyuridine incorporation and ELISA, respectively. Exposure of ASM to H 2 S donors inhibited FCS-induced proliferation and cytokine release, but was less effective upon COPD ASM cells compared to the non-smokers and smokers. The mRNA and protein expression of the enzymes responsible for endogenous H 2 S production (cystathionine-β-synthase [CBS] and 3-mercaptopyruvate sulphur transferase [MPST]) were inhibited by H 2 S donors. Finally, we report that exogenous H 2 S inhibited FCS-stimulated phosphorylation of ERK-1/2 and p38 mitogen activated protein kinases (MAPKs), in the non-smoker and smoker ASM cells, with little effect in COPD cells. H 2 S production provides a novel mechanism for the repression of ASM proliferation and cytokine release. The ability of COPD ASM cells to respond to H 2 S is attenuated in COPD ASM cells despite the presence of the enzymes responsible for H 2 S production.

Elevation of Serum Acid Sphingomyelinase Activity in Acute Kawasaki Disease.

PubMed

Konno, Yuuki; Takahashi, Ikuko; Narita, Ayuko; Takeda, Osamu; Koizumi, Hiromi; Tamura, Masamichi; Kikuchi, Wataru; Komatsu, Akira; Tamura, Hiroaki; Tsuchida, Satoko; Noguchi, Atsuko; Takahashi, Tsutomu

2015-10-01

Kawasaki disease (KD) is an acute systemic vasculitis that affects both small and medium-sized vessels including the coronary arteries in infants and children. Acid sphingomyelinase (ASM) is a lysosomal glycoprotein that hydrolyzes sphingomyelin to ceramide, a lipid, that functions as a second messenger in the regulation of cell functions. ASM activation has been implicated in numerous cellular stress responses and is associated with cellular ASM secretion, either through alternative trafficking of the ASM precursor protein or by means of an unidentified mechanism. Elevation of serum ASM activity has been described in several human diseases, suggesting that patients with diseases involving vascular endothelial cells may exhibit a preferential elevation of serum ASM activity. As acute KD is characterized by systemic vasculitis that could affect vascular endothelial cells, the elevation of serum ASM activity should be considered in these patients. In the present study, serum ASM activity in the sera of 15 patients with acute KD was determined both before and after treatment with infusion of high-dose intravenous immunoglobulin (IVIG), a first-line treatment for acute KD. Serum ASM activity before IVIG was significantly elevated in KD patients when compared to the control group (3.85 ± 1.46 nmol/0.1 ml/6 h vs. 1.15 ± 0.10 nmol/0.1 ml/6 h, p < 0.001), suggesting that ASM activation may be involved in the pathophysiology of this condition. Serum ASM activity before IVIG was significantly correlated with levels of C-reactive protein (p < 0.05). These results suggest the involvement of sphingolipid metabolism in the pathophysiology of KD.

EDS V25 containment vessel explosive qualification test report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rudolphi, John Joseph

2012-04-01

The V25 containment vessel was procured by the Project Manager, Non-Stockpile Chemical Materiel (PMNSCM) as a replacement vessel for use on the P2 Explosive Destruction Systems. It is the first EDS vessel to be fabricated under Code Case 2564 of the ASME Boiler and Pressure Vessel Code, which provides rules for the design of impulsively loaded vessels. The explosive rating for the vessel based on the Code Case is nine (9) pounds TNT-equivalent for up to 637 detonations. This limit is an increase from the 4.8 pounds TNT-equivalency rating for previous vessels. This report describes the explosive qualification tests thatmore » were performed in the vessel as part of the process for qualifying the vessel for explosive use. The tests consisted of a 11.25 pound TNT equivalent bare charge detonation followed by a 9 pound TNT equivalent detonation.« less

High Efficiency and Low Cost Thermal Energy Storage System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sienicki, James J.; Lv, Qiuping; Moisseytsev, Anton

BgtL, LLC (BgtL) is focused on developing and commercializing its proprietary compact technology for processes in the energy sector. One such application is a compact high efficiency Thermal Energy Storage (TES) system that utilizes the heat of fusion through phase change between solid and liquid to store and release energy at high temperatures and incorporate state-of-the-art insulation to minimize heat dissipation. BgtL’s TES system would greatly improve the economics of existing nuclear and coal-fired power plants by allowing the power plant to store energy when power prices are low and sell power into the grid when prices are high. Comparedmore » to existing battery storage technology, BgtL’s novel thermal energy storage solution can be significantly less costly to acquire and maintain, does not have any waste or environmental emissions, and does not deteriorate over time; it can keep constant efficiency and operates cleanly and safely. BgtL’s engineers are experienced in this field and are able to design and engineer such a system to a specific power plant’s requirements. BgtL also has a strong manufacturing partner to fabricate the system such that it qualifies for an ASME code stamp. BgtL’s vision is to be the leading provider of compact systems for various applications including energy storage. BgtL requests that all technical information about the TES designs be protected as proprietary information. To honor that request, only non-proprietay summaries are included in this report.« less

The Pivotal Role of Airway Smooth Muscle in Asthma Pathophysiology

PubMed Central

Ozier, Annaïg; Allard, Benoit; Bara, Imane; Girodet, Pierre-Olivier; Trian, Thomas; Marthan, Roger; Berger, Patrick

2011-01-01

Asthma is characterized by the association of airway hyperresponsiveness (AHR), inflammation, and remodelling. The aim of the present article is to review the pivotal role of airway smooth muscle (ASM) in the pathophysiology of asthma. ASM is the main effector of AHR. The mechanisms of AHR in asthma may involve a larger release of contractile mediators and/or a lower release of relaxant mediators, an improved ASM cell excitation/contraction coupling, and/or an alteration in the contraction/load coupling. Beyond its contractile function, ASM is also involved in bronchial inflammation and remodelling. Whereas ASM is a target of the inflammatory process, it can also display proinflammatory and immunomodulatory functions, through its synthetic properties and the expression of a wide range of cell surface molecules. ASM remodelling represents a key feature of asthmatic bronchial remodelling. ASM also plays a role in promoting complementary airway structural alterations, in particular by its synthetic function. PMID:22220184

Chloride channel blockers promote relaxation of TEA-induced contraction in airway smooth muscle.

PubMed

Yim, Peter D; Gallos, George; Perez-Zoghbi, Jose F; Trice, Jacquelyn; Zhang, Yi; Siviski, Matthew; Sonett, Joshua; Emala, Charles W

2013-01-01

Enhanced airway smooth muscle (ASM) contraction is an important component in the pathophysiology of asthma. We have shown that ligand gated chloride channels modulate ASM contractile tone during the maintenance phase of an induced contraction, however the role of chloride flux in depolarization-induced contraction remains incompletely understood. To better understand the role of chloride flux under these conditions, muscle force (human ASM, guinea pig ASM), peripheral small airway luminal area (rat ASM) and airway smooth muscle plasma membrane electrical potentials (human cultured ASM) were measured. We found ex vivo guinea pig airway rings, human ASM strips and small peripheral airways in rat lungs slices relaxed in response to niflumic acid following depolarization-induced contraction induced by K(+) channel blockade with tetraethylammonium chloride (TEA). In isolated human airway smooth muscle cells TEA induce depolarization as measured by a fluorescent indicator or whole cell patch clamp and this depolarization was reversed by niflumic acid. These findings demonstrate that ASM depolarization induced contraction is dependent on chloride channel activity. Targeting of chloride channels may be a novel approach to relax hypercontractile airway smooth muscle in bronchoconstrictive disorders.

Acid sphingomyelinase mediates human CD4+ T-cell signaling: potential roles in T-cell responses and diseases

PubMed Central

Bai, Aiping; Guo, Yuan

2017-01-01

Acid sphingomyelinase (ASM) is a lipid hydrolase. By generating ceramide, ASM had been reported to have an important role in regulating immune cell functions inclusive of macrophages, NK cells, and CD8+ T cells, whereas the role of ASM bioactivity in regulation of human CD4+ T-cell functions remained uncertain. Recent studies have provided novel findings in this field. Upon stimulation of CD3 and/or CD28, ASM-dependent ceramide signaling mediates intracellular downstream signal cascades of CD3 and CD28, and regulates CD4+ T-cell activation and proliferation. Meanwhile, CD39 and CD161 have direct interactions with ASM, which mediates downstream signals inclusive of STAT3 and mTOR and thus defines human Th17 cells. Intriguingly, ASM mediates Th1 responses, but negatively regulates Treg functions. In this review, we summarized the pivotal roles of ASM in regulation of human CD4+ T-cell activation and responses. ASM/sphingolipid signaling may be a novel target for the therapy of human autoimmune diseases. PMID:28749465

Acid Sphingomyelinase Gene Deficiency Ameliorates the Hyperhomocysteinemia-Induced Glomerular Injury in Mice

PubMed Central

Boini, Krishna M.; Xia, Min; Li, Caixia; Zhang, Chun; Payne, Lori P.; Abais, Justine M.; Poklis, Justin L.; Hylemon, Philip B.; Li, Pin-Lan

2011-01-01

Hyperhomocysteinemia (hHcys) enhances ceramide production, leading to the activation of NADPH oxidase and consequent glomerular oxidative stress and sclerosis. The present study was performed to determine whether acid sphingomyelinase (Asm), a ceramide-producing enzyme, is implicated in the development of hHcys-induced glomerular oxidative stress and injury. Uninephrectomized Asm-knockout (Asm−/−) and wild-type (Asm+/+) mice, with or without Asm short hairpin RNA (shRNA) transfection, were fed a folate-free (FF) diet for 8 weeks, which significantly elevated the plasma Hcys level compared with mice fed normal chow. By using in vivo molecular imaging, we found that transfected shRNAs were expressed in the renal cortex starting on day 3 and continued for 24 days. The FF diet significantly increased renal ceramide production, Asm mRNA and activity, urinary total protein and albumin excretion, glomerular damage index, and NADPH-dependent superoxide production in the renal cortex from Asm+/+ mice compared with that from Asm−/− or Asm shRNA-transfected wild-type mice. Immunofluorescence analysis showed that the FF diet decreased the expression of podocin but increased desmin and ceramide levels in glomeruli from Asm+/+ mice but not in those from Asm−/− and Asm shRNA-transfected wild-type mice. In conclusion, our observations reveal that Asm plays a pivotal role in mediating podocyte injury and glomerular sclerosis associated with NADPH oxidase–associated local oxidative stress during hHcys. PMID:21893018

Instigation of NLRP3 inflammasome activation and glomerular injury in mice on the high fat diet: role of acid sphingomyelinase gene

PubMed Central

Boini, Krishna M.; Xia, Min; Koka, Saisudha; Gehr, Todd W.; Li, Pin-Lan

2016-01-01

Ceramide has been reported to initiate inflammasome formation and activation in obesity and different pathological conditions. The present study was performed to explore the role of acid sphingomyelinase (Asm) in the development of high fat diet (HFD)-induced inflammasome and activation and consequent glomerular injury. Asm knockout (Asm−/−) and wild type (Asm+/+) mice with or without Asm short hairpin RNA (shRNA) transfection were fed a HFD or normal chow for 12 weeks to produce obesity and associated glomerular injury. HFD significantly enhanced the Asm activity, ceramide production, colocalization of Nlrp3 (Nod-like receptor protein 3) with ASC (apoptosis-associated speck-like protein) or Caspase-1, NADPH-dependent superoxide (O2•−) production in glomeruli of Asm+/+mice than in control diet-fed mice. However, such HFD-induced increases in Asm activity, ceramide production, colocalization of Nlrp3 with ASC or Caspase-1, superoxide (O2•−) production was attenuated in Asm−/− or Asm shRNA-transfected wild-type mice. In consistency with decreased inflammasome formation, the caspase-1 activity and IL-1β production was significantly attenuated in Asm−/− or Asm shRNA-transfected wild-type mice fed a HFD. Morphological examinations showed that HFD-induced profound injury in glomeruli of Asm+/+ mice which was markedly attenuated in Asm−/− mice. The decreased glomerular damage index in Asm−/− mice was accompanied by attenuated proteinuria. Fluorescent immunohistochemical examinations using podocin as a podocyte marker showed that inflammasome formation induced by the HFD were mostly located in podocytes as demonstrated by co-localization of podocin with Nlrp3. In conclusion, these observations disclose a pivotal role of Asm in the HFD-induced inflammasome formation and consequent glomerular inflammation and injury. PMID:26980705

Decreased muscle mass is not an independent risk factor for metabolic syndrome in Korean population aged 70 or older.

PubMed

Koo, Hyung Suk; Kim, Moon Jong; Kim, Kwang-Min; Kim, Young-Sang

2015-04-01

The association of low muscle mass with cardiometabolic risks is still controversial. The aim of this study was to investigate the relationship between low muscle mass and metabolic syndrome (MetS) according to the various muscle mass indices and to evaluate the influence of muscle mass on MetS independent of fat mass. Cross-sectional study About 841 men and 1106 women aged 70 or older from Korea National Health and Nutrition Examination Survey 2008-2010 MEASUREMENTS: We used various muscle mass indices: appendicular skeletal muscle mass (ASM) divided by height squared (ASM/Ht(2) ), ASM divided by body weight (ASM/Wt) and ASM adjusted for height and fat mass (residual). Low muscle mass is defined as ASM/Ht(2) and ASM/Wt below 2 SD of the sex-specific mean for healthy young adults. The sex-specific lowest quintile of the distribution of the residual was regarded as low muscle mass. The prevalence of MetS was higher in the population with low muscle mass defined by ASM/Wt, but lower in those defined by ASM/Ht(2) . However, after stratification according to the central obesity, low muscle mass was barely related with MetS. Meanwhile, when both ASM and fat mass were included in a logistic regression model, the odds ratios of 1 SD change of ASM for MetS were 1·07 (0·85-1·34) for men and 1·24 (1·04-1·47) for women, respectively. The relationship between low muscle mass and MetS was different according to the various muscle mass indices. After controlling the influence of fat mass, decreased muscle mass was not an independent risk factor for MetS. © 2014 John Wiley & Sons Ltd.

A Compliant Mechanism Synthesis Theory for Fostering Innovation of Micro Air Vehicles

DTIC Science & Technology

2016-04-01

ASME 2015 International Design Engineering Technical Conferences. Boston, MA. (2015): DETC201547271. 9. She, Y., Su, H.- J ., and Hurd, C ., " Shape ...Distribution approved for public release. [7] Klaptocz, A., Nicoud, J .-D., Floreano, D., Zufferey, J .- C ., Srinivasan, M., and Ellington, C ., 2010...inspired by insect flight”. In Flying Insects and Robots, D. Floreano, J .- C . Zufferey, M. V. Srinivasan, and C . Ellington, eds. Springer Berlin Heidelberg

Multi-angular Flame Measurements and Analysis in a Supersonic Wind Tunnel Using Fiber-Based Endoscopes

DTIC Science & Technology

2016-09-14

angular Flame Measurements and Analysis in a Supersonic Wind Tunnel Using Fiber-Based Endoscopes This paper reports new measurements and analysis made in...the Research Cell 19 super- sonic wind -tunnel facility housed at the Air Force Research Laboratory. The measure- ments include planar chemiluminescence...ASME for publication in the JOURNAL OF ENGINEERING FOR GAS TURBINES AND POWER. Manuscript received July 14, 2015; final manuscript received July 30

Turbulent Dispersion of Film Coolant and Hot Streaks in a Turbine Vane Cascade

DTIC Science & Technology

2015-01-18

of Film Coolant in a Turbine Vane Cascade, ASME 2014 International Gas Turbine Institute (10 2013) TOTAL: 1 Books Number of Manuscripts: Patents...Stanford, CA 94305 eatonj@stanford.edu (650) 723-1971 Overview High pressure turbine blades in gas turbine engines are exposed to extremely harsh...results are applicable to real gas turbines . The latter goal led to the development of a second experimental configuration that was not in the

Improved Problem Formulation in Engineering Systems Design

DTIC Science & Technology

1990-05-01

Journal model is ASME Journal of Mechanical Design. 2 1.1 Research Objective and Methods The objective of this research project was to improve...Methods and Theories, Journal of Design Methods Group, Vol. 20, No. 2, 1986, p. 410. 19 Nadler, G., Smith, J. M. and Frey, C. E., "Problem...sources do you routinely use to gather the information you need to develop a packaging system? 26 Trade journals 6 Consultants 21 Reference books 26

75 FR 40026 - Petition for Waiver of Compliance

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-13

...) and Alternative Safety Measures (ASM) as follows: 1 crossing closure (SSM), 1 crossing with gates and... channelization (ASM). The 2 SSMs will be completed by June 24, 2010, and the ASM will be completed within 45 working days of receipt of the ASM approval from FRA. In the future, 2 other crossings will be modified as...

Airway mechanics and methods used to visualize smooth muscle dynamics in vitro.

PubMed

Cooper, P R; McParland, B E; Mitchell, H W; Noble, P B; Politi, A Z; Ressmeyer, A R; West, A R

2009-10-01

Contraction of airway smooth muscle (ASM) is regulated by the physiological, structural and mechanical environment in the lung. We review two in vitro techniques, lung slices and airway segment preparations, that enable in situ ASM contraction and airway narrowing to be visualized. Lung slices and airway segment approaches bridge a gap between cell culture and isolated ASM, and whole animal studies. Imaging techniques enable key upstream events involved in airway narrowing, such as ASM cell signalling and structural and mechanical events impinging on ASM, to be investigated.

A review of biotransport education in the 21st century: lessons learned from experts.

PubMed

Banerjee, Rupak K; D'Souza, Gavin A; Rylander, Christopher; Devireddy, Ram

2014-11-01

The field of bioengineering is relatively new and complex including multiple disciplines encompassing areas in science and engineering. Efforts including the National Science Foundation (NSF) sponsored Integrative Graduate Education and Research Traineeship (IGERT) and VaNTH Engineering Research Center in Bioengineering Educational Technologies have been made to establish and disseminate knowledge and proven methods for teaching bioengineering concepts. Further, the summer bioengineering conference (SBC), sponsored by the American Society of Mechanical Engineers' (ASME) Bioengineering Division, was established to provide a meeting place for engineering educators and students having common interests in biological systems. Of the many subdisciplines of bioengineering, biotransport is a key subject that has wide applicability to many issues in engineering, biology, medicine, pharmacology, and environmental science, among others. The absence of standard content, guidelines, and texts needed for teaching biotransport courses to students motivated the Biotransport committee of ASME's Bioengineering Division to establish a biotransport education initiative. Biotransport education workshop sessions were conducted during the SBC 2011, 2012, and 2013 as part of this initiative. The workshop sessions included presentations from experienced faculty covering a spectrum of information from general descriptions of undergraduate biotransport courses to very detailed outlines of graduate courses to successful teaching techniques. A list of texts and references available for teaching biotransport courses at undergraduate and graduate levels has been collated and documented based on the workshop presentations. Further, based on individual teaching experiences and methodologies shared by the presenters, it was noted that active learning techniques, including cooperative and collaborative learning, can be useful for teaching undergraduate courses while problem based learning (PBL) can be a beneficial method for graduate courses. The outcomes of the education initiative will help produce students who are knowledgeable in the subject of biotransport, facile in applying biotransport concepts for solving problems in various application areas, and comfortable with their own abilities as life-long learners.

PAB3D Simulations for the CAWAPI F-16XL

NASA Technical Reports Server (NTRS)

Elmiligui, Alaa; Abdol-Hamid, K. S.; Massey, Steven J.

2007-01-01

Numerical simulations of the flow around F-16XL are performed as a contribution to the Cranked Arrow Wing Aerodynamic Project International (CAWAPI) using the PAB3D CFD code. Two turbulence models are used in the calculations: a standard k-! model, and the Shih-Zhu-Lumley (SZL) algebraic stress model. Seven flight conditions are simulated for the flow around the F-16XL where the free stream Mach number varies from 0.242 to 0.97. The range of angles of attack varies from 0deg to 20deg. Computational results, surface static pressure, boundary layer velocity profiles, and skin friction are presented and compared with flight data. Numerical results are generally in good agreement with flight data, considering that only one grid resolution is utilized for the different flight conditions simulated in this study. The ASM results are closer to the flight data than the k-! model results. The ASM predicted a stronger primary vortex, however, the origin of the vortex and footprint is approximately the same as in the k-! predictions.

Phosphodiesterases regulate airway smooth muscle function in health and disease.

PubMed

Krymskaya, Vera P; Panettieri, Reynold A

2007-01-01

On the basis of structure, regulation, and kinetic properties, phosphodiesterases (PDEs) represent a superfamily of enzymes divided into 11 subfamilies that catalyze cytosolic levels of 3',5'-cyclic adenosine monophosphate (cAMP) or 3',5'-cyclic guanosine monophosphate (cGMP) to 5'-AMP or 5'-GMP, respectively. PDE4 represents the major PDE expressed in inflammatory cells as well as airway smooth muscle (ASM), and selective PDE4 inhibitors provide a broad spectrum of anti-inflammatory effects such as abrogating cytokine and chemokine release from inflammatory cells and inhibiting inflammatory cell trafficking. Due to cell- and tissue-specific gene expression and regulation, PDEs modulate unique organ-based functions. New tools or compounds that selectively inhibit PDE subfamilies and genetically engineered mice deficient in selective isoforms have greatly enhanced our understanding of PDE function in airway inflammation and resident cell function. This chapter will focus on recent advances in our understanding of the role of PDE in regulating ASM function.

The Expression of NOX4 in Smooth Muscles of Small Airway Correlates with the Disease Severity of COPD

PubMed Central

2016-01-01

Airway smooth muscle (ASM) remodeling is a hallmark in chronic obstructive pulmonary disease (COPD), and nicotinamide-adenine dinucleotide phosphate (NADPH) oxidases (NOXs) produced reactive oxygen species (ROS) play a crucial role in COPD pathogenesis. In the present study, the expression of NOX4 and its correlation with the ASM hypertrophy/hyperplasia, clinical pulmonary functions, and the expression of transforming growth factor β (TGF-β) in the ASM of COPD small airways were investigated by semiquantitative morphological and/or immunohistochemistry staining methods. The results showed that an elevated expression of NOX4 and TGF-β, along with an increased volume of ASM mass, was found in the ASM of small airways in COPD patients. The abundance of NOX4 protein in the ASM was increased with disease severity and inversely correlated with the pulmonary functions in COPD patients. In addition, the expression of NOX4 and ASM marker α-SMA was colocalized, and the increased NOX4 expression was found to accompany an upregulated expression of TGF-β in the ASM of small airways of COPD lung. These results indicate that NOX4 may be a key regulator in ASM remodeling of small airway, in part through a mechanism interacting with TGF-β signaling in the pathogenesis of COPD, which warrants further investigation. PMID:27656649

Interaction between endoplasmic/sarcoplasmic reticulum stress (ER/SR stress), mitochondrial signaling and Ca(2+) regulation in airway smooth muscle (ASM).

PubMed

Delmotte, Philippe; Sieck, Gary C

2015-02-01

Airway inflammation is a key aspect of diseases such as asthma. Several inflammatory cytokines (e.g., TNFα and IL-13) increase cytosolic Ca(2+) ([Ca(2+)]cyt) responses to agonist stimulation and Ca(2+) sensitivity of force generation, thereby enhancing airway smooth muscle (ASM) contractility (hyper-reactive state). Inflammation also induces ASM proliferation and remodeling (synthetic state). In normal ASM, the transient elevation of [Ca(2+)]cyt induced by agonists leads to a transient increase in mitochondrial Ca(2+) ([Ca(2+)]mito) that may be important in matching ATP production with ATP consumption. In human ASM (hASM) exposed to TNFα and IL-13, the transient increase in [Ca(2+)]mito is blunted despite enhanced [Ca(2+)]cyt responses. We also found that TNFα and IL-13 induce reactive oxidant species (ROS) formation and endoplasmic/sarcoplasmic reticulum (ER/SR) stress (unfolded protein response) in hASM. ER/SR stress in hASM is associated with disruption of mitochondrial coupling with the ER/SR membrane, which relates to reduced mitofusin 2 (Mfn2) expression. Thus, in hASM it appears that TNFα and IL-13 result in ROS formation leading to ER/SR stress, reduced Mfn2 expression, disruption of mitochondrion-ER/SR coupling, decreased mitochondrial Ca(2+) buffering, mitochondrial fragmentation, and increased cell proliferation.

Smooth muscle in human bronchi is disposed to resist airway distension.

PubMed

Gazzola, Morgan; Henry, Cyndi; Couture, Christian; Marsolais, David; King, Gregory G; Fredberg, Jeffrey J; Bossé, Ynuk

2016-07-15

Studying airway smooth muscle (ASM) in conditions that emulate the in vivo environment within which the bronchi normally operate may provide important clues regarding its elusive physiological function. The present study examines the effect of lengthening and shortening of ASM on tension development in human bronchial segments. ASM from each bronchial segment was set at a length approximating in situ length (Linsitu). Bronchial tension was then measured during a slow cyclical strain (0.004Hz, from 0.7Linsitu to 1.3Linsitu) in the relaxed state and at graded levels of activation by methacholine. In all cases, tension was greater at longer ASM lengths, and greater during lengthening than shortening. The threshold of methacholine concentration that was required for ASM to account for bronchial tension across the entire range of ASM lengths tested was on average smaller by 2.8 logs during lengthening than during shortening. The length-dependency of ASM tension, together with this lower threshold of methacholine concentration during lengthening versus shortening, suggest that ASM has a greater ability to resist airway dilation during lung inflation than to narrow the airways during lung deflation. More than serving to narrow the airway, as has long been thought, these data suggest that the main function of ASM contraction is to limit airway wall distension during lung inflation. Copyright © 2016 Elsevier B.V. All rights reserved.

Interaction between endoplasmic/sarcoplasmic reticulum stress (ER/SR stress), mitochondrial signaling and Ca2+ regulation in airway smooth muscle (ASM)1

PubMed Central

Delmotte, Philippe; Sieck, Gary C.

2015-01-01

Airway inflammation is a key aspect of diseases such as asthma. Several inflammatory cytokines (e.g., TNFα and IL-13) increase cytosolic Ca2+ ([Ca2+]cyt) responses to agonist stimulation and Ca2+ sensitivity of force generation, thereby enhancing airway smooth muscle (ASM) contractility (hyper-reactive state). Inflammation also induces ASM proliferation and remodeling (synthetic state). In normal ASM, the transient elevation of [Ca2+]cyt induced by agonists leads to a transient increase in mitochondrial Ca2+ ([Ca2+]mito) that may be important in matching ATP production with ATP consumption. In human ASM (hASM) exposed to TNFα and IL-13, the transient increase in [Ca2+]mito is blunted despite enhanced [Ca2+]cyt responses. We also found that TNFα and IL-13 induce reactive oxidant species (ROS) formation and endoplasmic/sarcoplasmic reticulum (ER/SR) stress (unfolded protein response) in hASM. ER/SR stress in hASM is associated with disruption of mitochondrial coupling with the ER/SR membrane, which relates to reduced mitofusin 2 (Mfn2) expression. Thus, in hASM it appears that TNFα and IL-13 result in ROS formation leading to ER/SR stress, reduced Mfn2 expression, disruption of mitochondrion–ER/SR coupling, decreased mitochondrial Ca2+ buffering, mitochondrial fragmentation, and increased cell proliferation. PMID:25506723

Pesticides

MedlinePlus

... Satellite Session 2018 ASM Agenda 2018 ASM Faculty Bios 2018 ASM Events for Residents and Students 2018 ... Purchase CME Board Review Course Overview Agenda Faculty Bios Fees and Registration Course Materials Continuing Ed Hotel ...

Application of the International Water Association activated sludge models to describe aerobic sludge digestion.

PubMed

Ghorbani, M; Eskicioglu, C

2011-12-01

Batch and semi-continuous flow aerobic digesters were used to stabilize thickened waste-activated sludge at different initial conditions and mean solids retention times. Under dynamic conditions, total suspended solids, volatile suspended solids (VSS) and total and particulate chemical oxygen demand (COD and PCOD) were monitored in the batch reactors and effluent from the semi-continuous flow reactors. Activated Sludge Model (ASM) no. 1 and ASM no. 3 were applied to measured data (calibration data set) to evaluate the consistency and performances of models at different flow regimes for digester COD and VSS modelling. The results indicated that both ASM1 and ASM3 predicted digester COD, VSS and PCOD concentrations well (R2, Ra2 > or = 0.93). Parameter estimation concluded that compared to ASM1, ASM3 parameters were more consistent across different batch and semi-continuous flow runs with different operating conditions. Model validation on a data set independent from the calibration data successfully predicted digester COD (R2 = 0.88) and VSS (R2 = 0.94) concentrations by ASM3, while ASM1 overestimated both reactor COD (R2 = 0.74) and VSS concentrations (R2 = 0.79) after 15 days of aerobic batch digestion.

Influences of volcano eruptions on Asian Summer Monsoon over the last 110 years.

PubMed

Ning, Liang; Liu, Jian; Sun, Weiyi

2017-02-16

Asian summer monsoon (ASM) precipitation is the primary water resource for agriculture in many Asian countries that have experienced rapid economic growth in recent decades, thus implying the necessity for further investigations on both the internal variability of the ASM and the influence of external factors on the ASM. Using long-term high-resolution (0.5° × 0.5°) observed precipitation data, contrary to previous studies on inter-annual timescale, we showed that over the last 110 years, volcanic eruptions have influenced ASM variations on an inter-decadal timescale via teleconnections with the Atlantic Multi-decadal Oscillation (AMO). This relationship was also confirmed by Coupled Model Intercomparison Program Phase 5 (CMIP5) model simulations. During the active volcanic eruption periods (1901-1935 and 1963-1993), significantly lower ASM precipitation was observed compared with that during the inactive volcanic eruption period (1936-1962). We found that during active volcanic eruption periods, which correspond to a negative AMO state, there is an anomalously weakened Walker circulation over the tropical Pacific that transports less moisture to the ASM region and subsequently reduces ASM precipitation. This new finding may help improve decadal predictions of future changes in the ASM.

Chloride channel blockers promote relaxation of TEA-induced contraction in airway smooth muscle

PubMed Central

Yim, Peter D.; Gallos, George; Perez-zoghbi, Jose F.; Trice, Jacquelyn; Zhang, Yi; Siviski, Matthew; Sonett, Joshua; Emala, Charles W.

2014-01-01

Enhanced airway smooth muscle (ASM) contraction is an important component in the pathophysiology of asthma. We have shown that ligand gated chloride channels modulate ASM contractile tone during the maintenance phase of an induced contraction, however the role of chloride flux in depolarization-induced contraction remains incompletely understood. To better understand the role of chloride flux under these conditions, muscle force (human ASM, guinea pig ASM), peripheral small airway luminal area (rat ASM) and airway smooth muscle plasma membrane electrical potentials (human cultured ASM) were measured. We found ex vivo guinea pig airway rings, human ASM strips and small peripheral airways in rat lungs slices relaxed in response to niflumic acid following depolarization-induced contraction induced by K+ channel blockade with tetraethylammonium chloride (TEA). In isolated human airway smooth muscle cells TEA induce depolarization as measured by a fluorescent indicator or whole cell patch clamp and this depolarization was reversed by niflumic acid. These findings demonstrate that ASM depolarization induced contraction is dependent on chloride channel activity. Targeting of chloride channels may be a novel approach to relax hypercontractile airway smooth muscle in bronchoconstrictive disorders. PMID:24662476

Stalagmite-inferred centennial variability of the Asian summer monsoon in southwest China between 58 and 79 ka BP

NASA Astrophysics Data System (ADS)

Zhang, Tao-Tao; Li, Ting-Yong; Cheng, Hai; Edwards, R. Lawrence; Shen, Chuan-Chou; Spötl, Christoph; Li, Hong-Chun; Han, Li-Yin; Li, Jun-Yun; Huang, Chun-Xia; Zhao, Xin

2017-03-01

We use a new spliced stalagmite oxygen isotope record from Yangkou Cave and Xinya Cave, Chongqing, southwest China, to reconstruct the centennial-millennial-scale changes in Asian Summer Monsoon (ASM) intensity between 58.0 and 79.3 thousand years before present (ka BP, before AD 1950). This multidecadally resolved record shows four strong ASM periods, corresponding to Greenland Interstadials (GIS) 17-20, and three weak ASM episodes, among which, the one starting at 61.5 ± 0.2 ka BP and ending at 59.4 ± 0.2 ka BP that may correlate with Heinrich Event 6. The close agreement of climate events between China and Greenland supports the notion that the ASM is dominantly governed by high-latitude forcings in the Northern Hemisphere. The short-lived interstadial GIS 18, however, lasted for over 3 kyr in the records derived from ASM region, reflecting a gradual decline of ASM intensity, which coincides with a millennial-scale warming trend in Antarctica. This suggests an additional forcing of the ASM by the Southern Hemisphere, which also affected GIS 8-12, H4 and H5, as shown by previous speleothem studies from the ASM region.

Influences of volcano eruptions on Asian Summer Monsoon over the last 110 years

NASA Astrophysics Data System (ADS)

Ning, Liang; Liu, Jian; Sun, Weiyi

2017-02-01

Asian summer monsoon (ASM) precipitation is the primary water resource for agriculture in many Asian countries that have experienced rapid economic growth in recent decades, thus implying the necessity for further investigations on both the internal variability of the ASM and the influence of external factors on the ASM. Using long-term high-resolution (0.5° × 0.5°) observed precipitation data, contrary to previous studies on inter-annual timescale, we showed that over the last 110 years, volcanic eruptions have influenced ASM variations on an inter-decadal timescale via teleconnections with the Atlantic Multi-decadal Oscillation (AMO). This relationship was also confirmed by Coupled Model Intercomparison Program Phase 5 (CMIP5) model simulations. During the active volcanic eruption periods (1901-1935 and 1963-1993), significantly lower ASM precipitation was observed compared with that during the inactive volcanic eruption period (1936-1962). We found that during active volcanic eruption periods, which correspond to a negative AMO state, there is an anomalously weakened Walker circulation over the tropical Pacific that transports less moisture to the ASM region and subsequently reduces ASM precipitation. This new finding may help improve decadal predictions of future changes in the ASM.

Regulation of dynein-mediated autophagosomes trafficking by ASM in CASMCs.

PubMed

Xu, Ming; Zhang, Qiufang; Li, Pin-Lan; Nguyen, Thaison; Li, Xiang; Zhang, Yang

2016-01-01

Acid sphingomyelinase (ASM; gene symbol Smpd1) has been shown to play a crucial role in autophagy maturation by controlling lysosomal fusion with autophagosomes in coronary arterial smooth muscle cells (CASMCs). However, the underlying molecular mechanism by which ASM controls autophagolysosomal fusion remains unknown. In primary cultured CASMCs, lysosomal Ca2+ induced by 7-ketocholesterol (7-Ket, an atherogenic stimulus and autophagy inducer) was markedly attenuated by ASM deficiency or TRPML1 gene silencing suggesting that ASM signaling is required for TRPML1 channel activity and subsequent lysosomal Ca(2+) release. In these CASMCs, ASM deficiency or TRPML1 gene silencing markedly inhibited 7-Ket-induced dynein activation. In addition, 7-Ket-induced autophagosome trafficking, an event associated with lysosomal Ca(2+) release and dynein activity, was significantly inhibited in ASM-deficient (Smpd1(-/-)) CASMCs compared to that in Smpd1(+/+) CASMCs. Finally, overexpression of TRPML1 proteins restored 7-Ket-induced lysosomal Ca(2+) release and autophagosome trafficking in Smpd1-/- CASMCs. Collectively, these results suggest that ASM plays a critical role in regulating lysosomal TRPML1-Ca(2+) signaling and subsequent dynein-mediated autophagosome trafficking, which leads its role in controlling autophagy maturation in CASMCs under atherogenic stimulation.

Discriminating sarcopenia in community-dwelling older women with high frequency of overweight/obesity: the São Paulo Ageing & Health Study (SPAH).

PubMed

Domiciano, D S; Figueiredo, C P; Lopes, J B; Caparbo, V F; Takayama, L; Menezes, P R; Bonfa, E; Pereira, R M R

2013-02-01

The criteria most used for the definition of sarcopenia, those based on the ratio between the appendicular skeletal muscle mass (ASM) and the square of the height (h(2)) underestimate prevalence in overweight/obese people whereas another criteria consider ASM adjusted for total fat mass. We have shown that ASM adjusted for fat seems to be more appropriate for sarcopenia diagnosis. Since the prevalence of overweight and obesity is a growing public health issue, the aim of this study was to evaluate the prevalence and risk factors associated with sarcopenia, based on these two criteria, among older women. Six hundred eleven community-dwelling women were evaluated by specific questionnaire including clinical data. Body composition and bone mineral density were evaluated by dual X-ray absorptiometry. Logistic regression models were used to identify factors independently related to sarcopenia by ASM/h(2) and ASM adjusted for total fat mass criteria. The prevalence of overweight/obesity was high (74.3 %). The frequency of sarcopenia was lower using the criteria of ASM/h(2) (3.7 %) than ASM adjusted for fat (19.9 %) (P < 0.0001). We also note that less than 5 %(1/23) of sarcopenic women, according to ASM/h(2), had overweight/obesity, whereas 60 % (74/122) of sarcopenic women by ASM adjusted for fat had this complication. Using ASM/h(2), the associated factors observed in regression models were femoral neck T-score (OR = 1.90; 95 % CI 1.06-3.41; P = 0.03) and current alcohol intake (OR = 4.13, 95 % CI 1.18-14.45, P = 0.03). In contrast, we have identified that creatinine (OR = 0.21; 95 % CI 0.07-0.63; P = 0.005) and the White race (OR = 1.81; 95 % CI 1.15-2.84; P = 0.01) showed a significant association with sarcopenia using ASM adjusted for fat. In women with overweight/obesity, ASM adjusted for fat seems to be the more appropriate criteria for sarcopenia diagnosis. This finding has relevant public health implications, considering the high prevalence of overweight/obesity in older women.

Cracking the Sugar Code by Mass Spectrometry - An Invited Perspective in Honor of Dr. Catherine E. Costello, Recipient of the 2017 ASMS Distinguished Contribution Award

NASA Astrophysics Data System (ADS)

Mirgorodskaya, Ekaterina; Karlsson, Niclas G.; Sihlbom, Carina; Larson, Göran; Nilsson, Carol L.

2018-04-01

The structural study of glycans and glycoconjugates is essential to assign their roles in homeostasis, health, and disease. Once dominated by nuclear magnetic resonance spectroscopy, mass spectrometric methods have become the preferred toolbox for the determination of glycan structures at high sensitivity. The patterns of such structures in different cellular states now allow us to interpret the sugar codes in health and disease, based on structure-function relationships. Dr. Catherine E. Costello was the 2017 recipient of the American Society for Mass Spectrometry's Distinguished Contribution Award. In this Perspective article, we describe her seminal work in a historical and geographical context and review the impact of her research accomplishments in the field. 8[Figure not available: see fulltext.

Welding procedure specification. Suppliment 1. Records of procedure qualification tests. Gas tungsten arc welding of aluminum alloys 1XXX and 3003 to 3004, 5052 and 5X54. [1060, 1100, and 3003 to 3004, 5052, 5154, and 5454

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wodtke, C.H.; Frizzell, D.R.; Plunkett, W.A.

1986-06-01

Procedure WPS-2202 is qualified under Section IX of the ASME Boiler and Pressure Vessel Code for gas tungsten arc welding of aluminum alloys 1060, 1100, and 3003 (P-21) to 3004, 5052, 5154, and 5454 (P-22), in thickness range 0.062 to 0.062 to 0.5 inch; filler metal is ER5356 (F-22); shielding gas is argon.

Cognitive and biochemical effects of monosodium glutamate and aspartame, administered individually and in combination in male albino mice.

PubMed

Abu-Taweel, Gasem M; A, Zyadah M; Ajarem, Jamaan S; Ahmad, Mohammad

2014-01-01

The present study was designed to investigate the in vivo effects of monosodium glutamate (MSG) and aspartame (ASM) individually and in combination on the cognitive behavior and biochemical parameters like neurotransmitters and oxidative stress indices in the brain tissue of mice. Forty male Swiss albino mice were randomly divided into four groups of ten each and were exposed to MSG and ASM through drinking water for one month. Group I was the control and was given normal tap water. Groups II and III received MSG (8 mg/kg) and ASM (32 mg/kg) respectively dissolved in tap water. Group IV received MSG and ASM together in the same doses. After the exposure period, the animals were subjected to cognitive behavioral tests in a shuttle box and a water maze. Thereafter, the animals were sacrificed and the neurotransmitters and oxidative stress indices were estimated in their forebrain tissue. Both MSG and ASM individually as well as in combination had significant disruptive effects on the cognitive responses, memory retention and learning capabilities of the mice in the order (MSG+ASM)>ASM>MSG. Furthermore, while MSG and ASM individually were unable to alter the brain neurotransmitters and the oxidative stress indices, their combination dose (MSG+ASM) decreased significantly the levels of neurotransmitters (dopamine and serotonin) and it also caused oxidative stress by increasing the lipid peroxides measured in the form of thiobarbituric acid-reactive substances (TBARS) and decreasing the level of total glutathione (GSH). Further studies are required to evaluate the synergistic effects of MSG and ASM on the neurotransmitters and oxidative stress indices and their involvement in cognitive dysfunctions. Copyright © 2014 Elsevier Inc. All rights reserved.

Defining a Role for Acid Sphingomyelinase in the p38/Interleukin-6 Pathway*

PubMed Central

Perry, David M.; Newcomb, Benjamin; Adada, Mohamad; Wu, Bill X.; Roddy, Patrick; Kitatani, Kazuyuki; Siskind, Leah; Obeid, Lina M.; Hannun, Yusuf A.

2014-01-01

Acid sphingomyelinase (ASM) is one of the key enzymes involved in regulating the metabolism of the bioactive sphingolipid ceramide in the sphingolipid salvage pathway, yet defining signaling pathways by which ASM exerts its effects has proven difficult. Previous literature has implicated sphingolipids in the regulation of cytokines such as interleukin-6 (IL-6), but the specific sphingolipid pathways and mechanisms involved in inflammatory signaling need to be further elucidated. In this work, we sought to define the role of ASM in IL-6 production because our previous work showed that a parallel pathway of ceramide metabolism, acid β-glucosidase 1, negatively regulates IL-6. First, silencing ASM with siRNA abrogated IL-6 production in response to the tumor promoter, 4β-phorbol 12-myristate 13-acetate (PMA), in MCF-7 cells, in distinction to acid β-glucosidase 1 and acid ceramidase, suggesting specialization of the pathways. Moreover, treating cells with siRNA to ASM or with the indirect pharmacologic inhibitor desipramine resulted in significant inhibition of TNFα- and PMA-induced IL-6 production in MDA-MB-231 and HeLa cells. Knockdown of ASM was found to significantly inhibit PMA-dependent IL-6 induction at the mRNA level, probably ruling out mechanisms of translation or secretion of IL-6. Further, ASM knockdown or desipramine blunted p38 MAPK activation in response to TNFα, revealing a key role for ASM in activating p38, a signaling pathway known to regulate IL-6 induction. Last, knockdown of ASM dramatically blunted invasion of HeLa and MDA-MB-231 cells through Matrigel. Taken together, these results demonstrate that ASM plays a critical role in p38 signaling and IL-6 synthesis with implications for tumor pathobiology. PMID:24951586

Overexpression of microRNA-375 impedes platelet-derived growth factor-induced proliferation and migration of human fetal airway smooth muscle cells by targeting Janus kinase 2.

PubMed

Ji, Yamei; Yang, Xin; Su, Huixia

2018-02-01

The abnormal proliferation and migration of airway smooth muscle (ASM) cells play a critical role in airway remodeling during the development of asthma. MicroRNAs (miRNAs) have emerged as critical regulators of ASM cell proliferation and migration in airway remodeling. In this study, we aimed to investigate the potential role of miR-375 in the regulation of platelet-derived growth factor (PDGF)-induced fetal ASM cell proliferation and migration. Our results showed that miR-375 expression was significantly decreased in fetal ASM cells that were treated with PDGF. Functional data showed that overexpression of miR-375 inhibited the proliferation and migration of fetal ASM cells, whereas inhibition of miR-375 enhanced the proliferation and migration of fetal ASM cells. The results of bioinformatics analysis and a dual-luciferase reporter assay showed that miR-375 binds directly to the 3'-untranslated region of Janus kinase 2 (JAK2). Further data confirmed that miR-375 negatively regulates the expression of JAK2 in fetal ASM cells. Moreover, miR-375 also impeded the PDGF-induced activation of signal transducer and activator of transcription 3 (STAT3) in fetal ASM cells. However, restoration of JAK2 expression partially reversed the inhibitory effect of miR-375 on fetal ASM cell proliferation and migration. Overall, our results demonstrate that miR-375 inhibits fetal ASM cell proliferation and migration by targeting JAK2/STAT3 signaling. Our study provides a potential therapeutic target for the development of novel treatment strategies for pediatric asthma. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

c-Abl Is an Upstream Regulator of Acid Sphingomyelinase in Apoptosis Induced by Inhibition of Integrins αvβ3 and αvβ5

PubMed Central

Cooper, Jason P.; Kang, Min H.; Erdreich-Epstein, Anat

2012-01-01

Inhibition of integrins αvβ3/αvβ5 by the cyclic function-blocking peptide, RGDfV (Arg-Gly-Asp-Phe-Val) can induce apoptosis in both normal cells and tumor cells. We show that RGDfV induced apoptosis in ECV-304 carcinoma cells, increased activity and mRNA expression of acid sphingomyelinase (ASM), and increased ceramides C16, C18∶0, C24∶0 and C24∶1 while decreasing the corresponding sphingomyelins. siRNA to ASM decreased RGDfV-induced apoptosis as measured by TUNEL, PARP cleavage, mitochondrial depolarization, and caspase-3 and caspase-8 activities, as well as by annexinV in a 3D collagen model. These findings indicate a causal role for ASM in RGDfV-induced apoptosis in ECV-304. We have shown that c-Abl, a non-receptor tyrosine kinase, also mediates RGDfV-induced apoptosis. However, c-Abl, has not been previously linked to ASM in any system. Here we show that STI-571 (imatinib, inhibitor of c-Abl) inhibited RGDfV-induced ASM activity. Furthermore, STI-571 and c-Abl-siRNA both inhibited RGDfV-induced increase in ASM mRNA, but ASM-siRNA did not affect c-Abl phosphorylation or expression, supporting that c-Abl regulates the RGDfV-induced increase in ASM expression. These studies implicate ASM as a mediator of apoptosis induced by inhibition of integrins αvβ3/αvβ5, and for the first time place c-Abl as an upstream regulator of ASM expression and activity. PMID:22879933

An RGS4-mediated phenotypic switch of bronchial smooth muscle cells promotes fixed airway obstruction in asthma.

PubMed

Damera, Gautam; Druey, Kirk M; Cooper, Philip R; Krymskaya, Vera P; Soberman, Roy J; Amrani, Yassine; Hoshi, Toshinori; Brightling, Christopher E; Panettieri, Reynold A

2012-01-01

In severe asthma, bronchodilator- and steroid-insensitive airflow obstruction develops through unknown mechanisms characterized by increased lung airway smooth muscle (ASM) mass and stiffness. We explored the role of a Regulator of G-protein Signaling protein (RGS4) in the ASM hyperplasia and reduced contractile capacity characteristic of advanced asthma. Using immunocytochemical staining, ASM expression of RGS4 was determined in endobronchial biopsies from healthy subjects and those from subjects with mild, moderate and severe asthma. Cell proliferation assays, agonist-induced calcium mobilization and bronchoconstriction were determined in cultured human ASM cells and in human precision cut lung slices. Using gain- and loss-of-function approaches, the precise role of RGS proteins was determined in stimulating human ASM proliferation and inhibiting bronchoconstriction. RGS4 expression was restricted to a subpopulation of ASM and was specifically upregulated by mitogens, which induced a hyperproliferative and hypocontractile ASM phenotype similar to that observed in recalcitrant asthma. RGS4 expression was markedly increased in bronchial smooth muscle of patients with severe asthma, and expression correlated significantly with reduced pulmonary function. Whereas RGS4 inhibited G protein-coupled receptor (GPCR)-mediated bronchoconstriction, unexpectedly RGS4 was required for PDGF-induced proliferation and sustained activation of PI3K, a mitogenic signaling molecule that regulates ASM proliferation. These studies indicate that increased RGS4 expression promotes a phenotypic switch of ASM, evoking irreversible airway obstruction in subjects with severe asthma.

Structural and functional analysis of the ASM p.Ala359Asp mutant that causes acid sphingomyelinase deficiency.

PubMed

Acuña, Mariana; Castro-Fernández, Víctor; Latorre, Mauricio; Castro, Juan; Schuchman, Edward H; Guixé, Victoria; González, Mauricio; Zanlungo, Silvana

2016-10-21

Niemann-Pick disease (NPD) type A and B are recessive hereditary disorders caused by deficiency in acid sphingomyelinase (ASM). The p.Ala359Asp mutation has been described in several patients but its functional and structural effects in the protein are unknown. In order to characterize this mutation, we modeled the three-dimensional ASM structure using the recent available crystal of the mammalian ASM as a template. We found that the p.Ala359Asp mutation is localized in the hydrophobic core and far from the sphingomyelin binding site. However, energy function calculations using statistical potentials indicate that the mutation causes a decrease in ASM stability. Therefore, we investigated the functional effect of the p.Ala359Asp mutation in ASM expression, secretion, localization and activity in human fibroblasts. We found a 3.8% residual ASM activity compared to the wild-type enzyme, without changes in the other parameters evaluated. These results support the hypothesis that the p.Ala359Asp mutation causes structural alterations in the hydrophobic environment where ASM is located, decreasing its enzymatic activity. A similar effect was observed in other previously described NPDB mutations located outside the active site of the enzyme. This work shows the first full size ASM mutant model describe at date, providing a complete analysis of the structural and functional effects of the p.Ala359Asp mutation over the stability and activity of the enzyme. Copyright © 2016 Elsevier Inc. All rights reserved.

3D automatic anatomy recognition based on iterative graph-cut-ASM

NASA Astrophysics Data System (ADS)

Chen, Xinjian; Udupa, Jayaram K.; Bagci, Ulas; Alavi, Abass; Torigian, Drew A.

2010-02-01

We call the computerized assistive process of recognizing, delineating, and quantifying organs and tissue regions in medical imaging, occurring automatically during clinical image interpretation, automatic anatomy recognition (AAR). The AAR system we are developing includes five main parts: model building, object recognition, object delineation, pathology detection, and organ system quantification. In this paper, we focus on the delineation part. For the modeling part, we employ the active shape model (ASM) strategy. For recognition and delineation, we integrate several hybrid strategies of combining purely image based methods with ASM. In this paper, an iterative Graph-Cut ASM (IGCASM) method is proposed for object delineation. An algorithm called GC-ASM was presented at this symposium last year for object delineation in 2D images which attempted to combine synergistically ASM and GC. Here, we extend this method to 3D medical image delineation. The IGCASM method effectively combines the rich statistical shape information embodied in ASM with the globally optimal delineation capability of the GC method. We propose a new GC cost function, which effectively integrates the specific image information with the ASM shape model information. The proposed methods are tested on a clinical abdominal CT data set. The preliminary results show that: (a) it is feasible to explicitly bring prior 3D statistical shape information into the GC framework; (b) the 3D IGCASM delineation method improves on ASM and GC and can provide practical operational time on clinical images.

Life Prediction of High Temperature Polymer Matrix Composites for Aircraft Engine and Airframe Applications

DTIC Science & Technology

2008-09-01

R.d6 *RVE V 0 , aVfd(t) or< d22(t)= ir =S(t) (18) RLR Similarly, it can be shown that the component of the damage in the X3-direction...at time t is. d33(t) = 2]aSm 20.d(t).R.dd V YRVE V 0 oVfdlt) (19) RLR It is evident from equations (18) and (19) that d22 =dyi =S(t) and

The Slotted Blade Axial-Flow Blower

DTIC Science & Technology

1955-09-01

YORK 18, NEW YORK w is|’ .THE SLOTTED BLADE AXIAL-FLOW BLOVER AUG 0 1 13941J F Dr. H. E. Sheets, Member ASME Chief Research and Development Engineer ... blades of an axial flow blower. The subject of boundary-layer control has attracted considerable attention in respect to the isolated airfoil (1)1 but... blades . Flow through airfoils displays a region of laminar flow beginning at the leading edge. Further downstream, at approximately the location of the

Model of Transition from Laminar to Turbulent Flow

NASA Astrophysics Data System (ADS)

Kanda, Hidesada

2001-11-01

For circular pipe flows, a model of transition from laminar to turbulent flow has already been proposed and the minimum critical Reynolds number of approximately 2040 was obtained (Kanda, 1999). In order to prove the validity of the model, another verification is required. Thus, for plane Poiseuille flow, results of previous investigations were studied, focusing on experimental data on the critical Reynolds number Rc, the entrance length, and the transition length. Consequently, concerning the natural transition, it was confirmed from the experimental data that (i) the transition occurs in the entrance region, (ii) Rc increases as the contraction ratio in the inlet section increases, and (iii) the minimum Rc is obtained when the contraction ratio is the smallest or one, and there is no-bellshaped entrance or straight parallel plates. Its value exists in the neighborhood of 1300, based on the channel height and the average velocity. Although, for Hagen-Poiseuille flow, the minimum Rc is approximately 2000, based on the pipe diameter and the average velocity, there seems to be no significant difference in the transition from laminar to turbulent flow between Hagen-Poiseuille flow and plane Poiseuille flow (Kanda, 2001). Rc is determined by the shape of the inlet. Kanda, H., 1999, Proc. of ASME Fluids Engineering Division - 1999, FED-Vol. 250, pp. 197-204. Kanda, H., 2001, Proc. of ASME Fluids Engineering Division - 2001.

Management Sciences Division Annual Report (10th)

DTIC Science & Technology

1993-01-01

of the Weapon System Management Information System (WSMIS). TheI Aircraft Sustainability Model ( ASM ) is the computational technique employed by...provisioning. We enhanced the capabilities of RBIRD by using the Aircraft Sustainability Model ( ASM ) for the spares calculation. ASM offers many... ASM for several years to 3 compute spares for war. It is also fully compatible with the Air Force’s peacetime spares computation system (D041). This

Regulation of hematogenous tumor metastasis by acid sphingomyelinase

PubMed Central

Carpinteiro, Alexander; Becker, Katrin Anne; Japtok, Lukasz; Hessler, Gabriele; Keitsch, Simone; Požgajovà, Miroslava; Schmid, Kurt W; Adams, Constantin; Müller, Stefan; Kleuser, Burkhard; Edwards, Michael J; Grassmé, Heike; Helfrich, Iris; Gulbins, Erich

2015-01-01

Metastatic dissemination of cancer cells is the ultimate hallmark of malignancy and accounts for approximately 90% of human cancer deaths. We investigated the role of acid sphingomyelinase (Asm) in the hematogenous metastasis of melanoma cells. Intravenous injection of B16F10 melanoma cells into wild-type mice resulted in multiple lung metastases, while Asm-deficient mice (Smpd1−/− mice) were protected from pulmonary tumor spread. Transplanting wild-type platelets into Asm-deficient mice reinstated tumor metastasis. Likewise, Asm-deficient mice were protected from hematogenous MT/ret melanoma metastasis to the spleen in a mouse model of spontaneous tumor metastasis. Human and mouse melanoma cells triggered activation and release of platelet secretory Asm, in turn leading to ceramide formation, clustering, and activation of α5β1 integrins on melanoma cells finally leading to adhesion of the tumor cells. Clustering of integrins by applying purified Asm or C16 ceramide to B16F10 melanoma cells before intravenous injection restored trapping of tumor cells in the lung in Asm-deficient mice. This effect was revertable by arginine-glycine-aspartic acid peptides, which are known inhibitors of integrins, and by antibodies neutralizing β1 integrins. These findings indicate that melanoma cells employ platelet-derived Asm for adhesion and metastasis. PMID:25851537

Influences of volcano eruptions on Asian Summer Monsoon over the last 110 years

PubMed Central

Ning, Liang; Liu, Jian; Sun, Weiyi

2017-01-01

Asian summer monsoon (ASM) precipitation is the primary water resource for agriculture in many Asian countries that have experienced rapid economic growth in recent decades, thus implying the necessity for further investigations on both the internal variability of the ASM and the influence of external factors on the ASM. Using long-term high-resolution (0.5° × 0.5°) observed precipitation data, contrary to previous studies on inter-annual timescale, we showed that over the last 110 years, volcanic eruptions have influenced ASM variations on an inter-decadal timescale via teleconnections with the Atlantic Multi-decadal Oscillation (AMO). This relationship was also confirmed by Coupled Model Intercomparison Program Phase 5 (CMIP5) model simulations. During the active volcanic eruption periods (1901–1935 and 1963–1993), significantly lower ASM precipitation was observed compared with that during the inactive volcanic eruption period (1936–1962). We found that during active volcanic eruption periods, which correspond to a negative AMO state, there is an anomalously weakened Walker circulation over the tropical Pacific that transports less moisture to the ASM region and subsequently reduces ASM precipitation. This new finding may help improve decadal predictions of future changes in the ASM. PMID:28205603

Molecular mechanisms underlying airway smooth muscle contraction and proliferation: implications for asthma.

PubMed

Pelaia, Girolamo; Renda, Teresa; Gallelli, Luca; Vatrella, Alessandro; Busceti, Maria Teresa; Agati, Sergio; Caputi, Mario; Cazzola, Mario; Maselli, Rosario; Marsico, Serafino A

2008-08-01

Airway smooth muscle (ASM) plays a key role in bronchomotor tone, as well as in structural remodeling of the bronchial wall. Therefore, ASM contraction and proliferation significantly participate in the development and progression of asthma. Many contractile agonists also behave as mitogenic stimuli, thus contributing to frame a hyperresponsive and hyperplastic ASM phenotype. In this review, the molecular mechanisms and signaling pathways involved in excitation-contraction coupling and ASM cell growth will be outlined. Indeed, the recent advances in understanding the basic aspects of ASM biology are disclosing important cellular targets, currently explored for the implementation of new, more effective anti-asthma therapies.

Acid sphingomyelinase modulates the autophagic process by controlling lysosomal biogenesis in Alzheimer's disease.

PubMed

Lee, Jong Kil; Jin, Hee Kyung; Park, Min Hee; Kim, Bo-ra; Lee, Phil Hyu; Nakauchi, Hiromitsu; Carter, Janet E; He, Xingxuan; Schuchman, Edward H; Bae, Jae-sung

2014-07-28

In Alzheimer's disease (AD), abnormal sphingolipid metabolism has been reported, although the pathogenic consequences of these changes have not been fully characterized. We show that acid sphingomyelinase (ASM) is increased in fibroblasts, brain, and/or plasma from patients with AD and in AD mice, leading to defective autophagic degradation due to lysosomal depletion. Partial genetic inhibition of ASM (ASM(+/-)) in a mouse model of familial AD (FAD; amyloid precursor protein [APP]/presenilin 1 [PS1]) ameliorated the autophagocytic defect by restoring lysosomal biogenesis, resulting in improved AD clinical and pathological findings, including reduction of amyloid-β (Aβ) deposition and improvement of memory impairment. Similar effects were noted after pharmacologic restoration of ASM to the normal range in APP/PS1 mice. Autophagic dysfunction in neurons derived from FAD patient induced pluripotent stem cells (iPSCs) was restored by partial ASM inhibition. Overall, these results reveal a novel mechanism of ASM pathogenesis in AD that leads to defective autophagy due to impaired lysosomal biogenesis and suggests that partial ASM inhibition is a potential new therapeutic intervention for the disease. © 2014 Lee et al.

S100A8 protein attenuates airway hyperresponsiveness by suppressing the contraction of airway smooth muscle.

PubMed

Xu, Yu-Dong; Wang, Yu; Yin, Lei-Miao; Park, Gyoung-Hee; Ulloa, Luis; Yang, Yong-Qing

2017-02-26

Airway hyperresponsiveness (AHR) is a major clinical problem in allergic asthma mainly caused by the hypercontractility of airway smooth muscles (ASM). S100A8 is an important member of the S100 calcium-binding protein family with a potential to regulate cell contractility. Here, we analyze the potential of S100A8 to regulate allergen-induced AHR and ASM contraction. Treatment with recombinant S100A8 (rS100A8) diminished airway hyperresponsiveness in OVA-sensitized rats. ASM contraction assays showed that rS100A8 reduced hypercontractility in both isolated tracheal rings and primary ASM cells treated by acetylcholine. rS100A8 markedly rescued the phosphorylation level of myosin light chain induced by acetylcholine in ASM cells. These results show that rS100A8 plays a protective role in regulating AHR in asthma by inhibiting ASM contraction. These results support S100A8 as a novel therapeutic target to control ASM contraction in asthma. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

A Minimal Path Searching Approach for Active Shape Model (ASM)-based Segmentation of the Lung.

PubMed

Guo, Shengwen; Fei, Baowei

2009-03-27

We are developing a minimal path searching method for active shape model (ASM)-based segmentation for detection of lung boundaries on digital radiographs. With the conventional ASM method, the position and shape parameters of the model points are iteratively refined and the target points are updated by the least Mahalanobis distance criterion. We propose an improved searching strategy that extends the searching points in a fan-shape region instead of along the normal direction. A minimal path (MP) deformable model is applied to drive the searching procedure. A statistical shape prior model is incorporated into the segmentation. In order to keep the smoothness of the shape, a smooth constraint is employed to the deformable model. To quantitatively assess the ASM-MP segmentation, we compare the automatic segmentation with manual segmentation for 72 lung digitized radiographs. The distance error between the ASM-MP and manual segmentation is 1.75 ± 0.33 pixels, while the error is 1.99 ± 0.45 pixels for the ASM. Our results demonstrate that our ASM-MP method can accurately segment the lung on digital radiographs.

Acid sphingomyelinase modulates the autophagic process by controlling lysosomal biogenesis in Alzheimer’s disease

PubMed Central

Lee, Jong Kil; Jin, Hee Kyung; Park, Min Hee; Kim, Bo-ra; Lee, Phil Hyu; Nakauchi, Hiromitsu; Carter, Janet E.; He, Xingxuan; Schuchman, Edward H.

2014-01-01

In Alzheimer’s disease (AD), abnormal sphingolipid metabolism has been reported, although the pathogenic consequences of these changes have not been fully characterized. We show that acid sphingomyelinase (ASM) is increased in fibroblasts, brain, and/or plasma from patients with AD and in AD mice, leading to defective autophagic degradation due to lysosomal depletion. Partial genetic inhibition of ASM (ASM+/−) in a mouse model of familial AD (FAD; amyloid precursor protein [APP]/presenilin 1 [PS1]) ameliorated the autophagocytic defect by restoring lysosomal biogenesis, resulting in improved AD clinical and pathological findings, including reduction of amyloid-β (Aβ) deposition and improvement of memory impairment. Similar effects were noted after pharmacologic restoration of ASM to the normal range in APP/PS1 mice. Autophagic dysfunction in neurons derived from FAD patient induced pluripotent stem cells (iPSCs) was restored by partial ASM inhibition. Overall, these results reveal a novel mechanism of ASM pathogenesis in AD that leads to defective autophagy due to impaired lysosomal biogenesis and suggests that partial ASM inhibition is a potential new therapeutic intervention for the disease. PMID:25049335

A minimal path searching approach for active shape model (ASM)-based segmentation of the lung

NASA Astrophysics Data System (ADS)

Guo, Shengwen; Fei, Baowei

2009-02-01

We are developing a minimal path searching method for active shape model (ASM)-based segmentation for detection of lung boundaries on digital radiographs. With the conventional ASM method, the position and shape parameters of the model points are iteratively refined and the target points are updated by the least Mahalanobis distance criterion. We propose an improved searching strategy that extends the searching points in a fan-shape region instead of along the normal direction. A minimal path (MP) deformable model is applied to drive the searching procedure. A statistical shape prior model is incorporated into the segmentation. In order to keep the smoothness of the shape, a smooth constraint is employed to the deformable model. To quantitatively assess the ASM-MP segmentation, we compare the automatic segmentation with manual segmentation for 72 lung digitized radiographs. The distance error between the ASM-MP and manual segmentation is 1.75 +/- 0.33 pixels, while the error is 1.99 +/- 0.45 pixels for the ASM. Our results demonstrate that our ASM-MP method can accurately segment the lung on digital radiographs.

Modelling High-temperature EBPR by Incorporating Glycogen and GAOs: Challenges from a Preliminary Study.

PubMed

Liau, Kee Fui; Yeoh, Hak Koon; Shoji, Tadashi; Chua, Adeline Seak May; Ho, Pei Yee

2017-01-01

  Recently reported kinetic and stoichiometric parameters of the Activated Sludge Model no. 2d (ASM2d) for high-temperature EBPR processes suggested that the absence of glycogen in the model contributed to underestimation of PHA accumulation at 32 °C. Here, two modified ASM2d models were used to further explore the contribution of glycogen in the process. The ASM2d-1G model incorporated glycogen metabolism by PAOs (polyphosphate-accumulating organisms), while the ASM2d-2G model further included processes by GAOs (glycogen-accumulating organisms). These models were calibrated and validated using experimental data at 32 °C. The ASM2d-1G model supported the hypothesis that the excess PHA was attributed to glycogen, but remained inadequate to capture the dynamics of glycogen without considering GAOs activities. The ASM2d-2G model performed better, but it was challenging to calibrate as it often led to wash-out of either PAOs or GAOs. Associated hurdles are highlighted and additional efforts in calibrating ASM2d-2G more effectively are proposed.

A Minimal Path Searching Approach for Active Shape Model (ASM)-based Segmentation of the Lung

PubMed Central

Guo, Shengwen; Fei, Baowei

2013-01-01

We are developing a minimal path searching method for active shape model (ASM)-based segmentation for detection of lung boundaries on digital radiographs. With the conventional ASM method, the position and shape parameters of the model points are iteratively refined and the target points are updated by the least Mahalanobis distance criterion. We propose an improved searching strategy that extends the searching points in a fan-shape region instead of along the normal direction. A minimal path (MP) deformable model is applied to drive the searching procedure. A statistical shape prior model is incorporated into the segmentation. In order to keep the smoothness of the shape, a smooth constraint is employed to the deformable model. To quantitatively assess the ASM-MP segmentation, we compare the automatic segmentation with manual segmentation for 72 lung digitized radiographs. The distance error between the ASM-MP and manual segmentation is 1.75 ± 0.33 pixels, while the error is 1.99 ± 0.45 pixels for the ASM. Our results demonstrate that our ASM-MP method can accurately segment the lung on digital radiographs. PMID:24386531

Experimental uncertainty and drag measurements in the national transonic facility

NASA Technical Reports Server (NTRS)

Batill, Stephen M.

1994-01-01

This report documents the results of a study which was conducted in order to establish a framework for the quantitative description of the uncertainty in measurements conducted in the National Transonic Facility (NTF). The importance of uncertainty analysis in both experiment planning and reporting results has grown significantly in the past few years. Various methodologies have been proposed and the engineering community appears to be 'converging' on certain accepted practices. The practical application of these methods to the complex wind tunnel testing environment at the NASA Langley Research Center was based upon terminology and methods established in the American National Standards Institute (ANSI) and the American Society of Mechanical Engineers (ASME) standards. The report overviews this methodology.

A Review & Assessment of Current Operating Conditions Allowable Stresses in ASME Section III Subsection NH

DOE Office of Scientific and Technical Information (OSTI.GOV)

R. W. Swindeman

2009-12-14

The current operating condition allowable stresses provided in ASME Section III, Subsection NH were reviewed for consistency with the criteria used to establish the stress allowables and with the allowable stresses provided in ASME Section II, Part D. It was found that the S{sub o} values in ASME III-NH were consistent with the S values in ASME IID for the five materials of interest. However, it was found that 0.80 S{sub r} was less than S{sub o} for some temperatures for four of the materials. Only values for alloy 800H appeared to be consistent with the criteria on which S{submore » o} values are established. With the intent of undertaking a more detailed evaluation of issues related to the allowable stresses in ASME III-NH, the availabilities of databases for the five materials were reviewed and augmented databases were assembled.« less

Integrin and GPCR Crosstalk in the Regulation of ASM Contraction Signaling in Asthma.

PubMed

Teoh, Chun Ming; Tam, John Kit Chung; Tran, Thai

2012-01-01

Airway hyperresponsiveness (AHR) is one of the cardinal features of asthma. Contraction of airway smooth muscle (ASM) cells that line the airway wall is thought to influence aspects of AHR, resulting in excessive narrowing or occlusion of the airway. ASM contraction is primarily controlled by agonists that bind G protein-coupled receptor (GPCR), which are expressed on ASM. Integrins also play a role in regulating ASM contraction signaling. As therapies for asthma are based on symptom relief, better understanding of the crosstalk between GPCRs and integrins holds good promise for the design of more effective therapies that target the underlying cellular and molecular mechanism that governs AHR. In this paper, we will review current knowledge about integrins and GPCRs in their regulation of ASM contraction signaling and discuss the emerging concept of crosstalk between the two and the implication of this crosstalk on the development of agents that target AHR.

Attenuation of airway smooth muscle contractility via flavonol-mediated inhibition of phospholipase-Cβ

PubMed Central

Brown, Amy; Danielsson, Jennifer; Townsend, Elizabeth A.; Zhang, Yi; Perez-Zoghbi, Jose F.; Emala, Charles W.

2016-01-01

Enhanced contractility of airway smooth muscle (ASM) is a major pathophysiological characteristic of asthma. Expanding the therapeutic armamentarium beyond β-agonists that target ASM hypercontractility would substantially improve treatment options. Recent studies have identified naturally occurring phytochemicals as candidates for acute ASM relaxation. Several flavonoids were evaluated for their ability to acutely relax human and murine ASM ex vivo and murine airways in vivo and were evaluated for their ability to inhibit procontractile signaling pathways in human ASM (hASM) cells. Two members of the flavonol subfamily, galangin and fisetin, significantly relaxed acetylcholine-precontracted murine tracheal rings ex vivo (n = 4 and n = 5, respectively, P < 0.001). Galangin and fisetin also relaxed acetylcholine-precontracted hASM strips ex vivo (n = 6–8, P < 0.001). Functional respiratory in vivo murine studies demonstrated that inhaled galangin attenuated the increase in lung resistance induced by inhaled methacholine (n = 6, P < 0.01). Both flavonols, galangin and fisetin, significantly inhibited purified phosphodiesterase-4 (PDE4) (n = 7, P < 0.05; n = 7, P < 0.05, respectively), and PLCβ enzymes (n = 6, P < 0.001 and n = 6, P < 0.001, respectively) attenuated procontractile Gq agonists' increase in intracellular calcium (n = 11, P < 0.001), acetylcholine-induced increases in inositol phosphates, and CPI-17 phosphorylation (n = 9, P < 0.01) in hASM cells. The prorelaxant effect retained in these structurally similar flavonols provides a novel pharmacological method for dual inhibition of PLCβ and PDE4 and therefore may serve as a potential treatment option for acute ASM constriction. PMID:26773068

Is the apical soft tissue margin a better predictor of biochemical recurrence than the surgical specimen?

PubMed

Godoy, Guilherme; Tareen, Basir U; Lepor, Herbert

2011-01-01

To identify predictors of apical surgical margin (ASM) and apical soft tissue margin (ASTM), determine if the ASTM is a better predictor of biochemical recurrence (BR) than the ASM, and ascertain the impact of apical biopsies on BR rates. One thousand three hundred eight consecutive men underwent open radical retropubic prostatectomy (RP) between October 2000 and December 2006. Circumferential biopsies of the ASTM were obtained intraoperatively and submitted for frozen section analysis. Logistic regression models were utilized to identify the factors associated with the presence of positive ASMs and ASTMs. The estimated 5-year risk of BR was calculated by the Kaplan-Meier method. Overall, 43 (3.3%) and 86 (6.6%) of cases exhibited positive ASM and ASTM, respectively. ASM was significantly associated with higher mean serum prostate-specific antigen levels, presence of perineural invasion, and greater volume of tumor in the biopsy specimen. None of these factors were observed to be associated with the presence of cancer in the ASTMs. In the multivariate analysis, only the presence of perineural invasion was a significant independent predictor of ASMs. The estimated 5-year BR rates in the positive ASMs only, ASTMs only, and both positive ASMs and ASTMs groups were 48.6%, 4.7%, and 38.8%, respectively. A positive ASM was associated with a significantly greater risk of BR compared with a positive ASTM. The very low estimated risk of BR at 5 years in cases with ASTM suggests that performing the ASTM biopsies may increase the cure rates achieved with RP. Copyright © 2011 Elsevier Inc. All rights reserved.

Attenuation of airway smooth muscle contractility via flavonol-mediated inhibition of phospholipase-Cβ.

PubMed

Brown, Amy; Danielsson, Jennifer; Townsend, Elizabeth A; Zhang, Yi; Perez-Zoghbi, Jose F; Emala, Charles W; Gallos, George

2016-04-15

Enhanced contractility of airway smooth muscle (ASM) is a major pathophysiological characteristic of asthma. Expanding the therapeutic armamentarium beyond β-agonists that target ASM hypercontractility would substantially improve treatment options. Recent studies have identified naturally occurring phytochemicals as candidates for acute ASM relaxation. Several flavonoids were evaluated for their ability to acutely relax human and murine ASM ex vivo and murine airways in vivo and were evaluated for their ability to inhibit procontractile signaling pathways in human ASM (hASM) cells. Two members of the flavonol subfamily, galangin and fisetin, significantly relaxed acetylcholine-precontracted murine tracheal rings ex vivo (n = 4 and n = 5, respectively, P < 0.001). Galangin and fisetin also relaxed acetylcholine-precontracted hASM strips ex vivo (n = 6-8, P < 0.001). Functional respiratory in vivo murine studies demonstrated that inhaled galangin attenuated the increase in lung resistance induced by inhaled methacholine (n = 6, P < 0.01). Both flavonols, galangin and fisetin, significantly inhibited purified phosphodiesterase-4 (PDE4) (n = 7, P < 0.05; n = 7, P < 0.05, respectively), and PLCβ enzymes (n = 6, P < 0.001 and n = 6, P < 0.001, respectively) attenuated procontractile Gq agonists' increase in intracellular calcium (n = 11, P < 0.001), acetylcholine-induced increases in inositol phosphates, and CPI-17 phosphorylation (n = 9, P < 0.01) in hASM cells. The prorelaxant effect retained in these structurally similar flavonols provides a novel pharmacological method for dual inhibition of PLCβ and PDE4 and therefore may serve as a potential treatment option for acute ASM constriction. Copyright © 2016 the American Physiological Society.

Establishment and characterization of an angiosarcoma-derived cell line, AS-M.

PubMed

Krump-Konvalinkova, Vera; Bittinger, Fernando; Olert, Jürgen; Bräuninger, Wolfgang; Brunner, Joachim; Kirkpatrick, C James

2003-01-01

A novel human endothelial cell line, AS-M, has been established from a cutaneous angiosarcoma on the scalp. The cells expressing platelet endothelial cell adhesion molecule-1 (CD31) were isolated using magnetic beads and subsequently cultured for a year. To date, the cells have undergone more than 100 population doublings (PDs). The AS-M cells manifested endothelial characteristics, such as active uptake of acetylated low-density lipoprotein labeled with 1,1'-dioctadecyl 3,3,3',3'-tetramethylindocarbocyanine perchlorate (Dil-Ac-LDL), capacity to bind the Ulex europeaus agglutin-I (UEA-I), and expression of von Willebrand factor (vWF) and CD31. The single cell-derived clone, AS-M.5, showed a constitutive expression of CD31, vWF, angiotensin-converting enzyme (ACE), endoglin (CD105), and the endothelial cell receptor tyrosine kinases KDR and Tie-1. Similarly to freshly isolated endothelial cells, the AS-M.5 responded to induction by bacterial lipopolysaccharide (LPS) by increased transcription of cell adhesion molecules and cytokines. The AS-M.5 cultures required endothelial growth supplements for optimal growth and long-term propagation in vitro. However, in contrast to normal endothelial cells, p53 gene products were detected in nuclei of AS-M.5 cells. Cytogenetic analyses consistently revealed a hypodiploid karyotype with complete loss of one homologue of several chromosomes and a homogeneous pattern of distinct karyotypic changes. Although the AS-M.5 presented characteristics suggestive of tumor cells, they did not develop into tumors when inoculated subcutaneously into nude mice. The cell line AS-M.5 could be a useful model system to study endothelial pathobiology in vitro.

Airway hyperresponsiveness; smooth muscle as the principal actor

PubMed Central

Lauzon, Anne-Marie; Martin, James G.

2016-01-01

Airway hyperresponsiveness (AHR) is a defining characteristic of asthma that refers to the capacity of the airways to undergo exaggerated narrowing in response to stimuli that do not result in comparable degrees of airway narrowing in healthy subjects. Airway smooth muscle (ASM) contraction mediates airway narrowing, but it remains uncertain as to whether the smooth muscle is intrinsically altered in asthmatic subjects or is responding abnormally as a result of the milieu in which it sits. ASM in the trachea or major bronchi does not differ in its contractile characteristics in asthmatics, but the more pertinent peripheral airways await complete exploration. The mass of ASM is increased in many but not all asthmatics and therefore cannot be a unifying hypothesis for AHR, although when increased in mass it may contribute to AHR. The inability of a deep breath to reverse or prevent bronchial narrowing in asthma may reflect an intrinsic difference in the mechanisms that lead to softening of contracted ASM when subjected to stretch. Cytokines such as interleukin-13 and tumor necrosis factor-α promote a more contractile ASM phenotype. The composition and increased stiffness of the matrix in which ASM is embedded promotes a more proliferative and pro-inflammatory ASM phenotype, but the expected dedifferentiation and loss of contractility have not been shown. Airway epithelium may drive ASM proliferation and/or molecular remodeling in ways that may lead to AHR. In conclusion, AHR is likely multifactorial in origin, reflecting the plasticity of ASM properties in the inflammatory environment of the asthmatic airway. PMID:26998246

Clinical predictors of advanced sellar masses.

PubMed

Rambaldini, Gloria M; Butalia, Sonia; Ezzat, Shereen; Kucharczyk, Walter; Sawka, Anna M

2007-10-01

To identify clinical variables associated with the presence of a structurally advanced sellar mass (ASM). We performed a retrospective study of patients referred for evaluation of suspected new pituitary disease or sellar mass to the Endocrine Oncology Unit of Mount Sinai Hospital in Toronto, Ontario, Canada. By multivariate analysis, we examined predictors of a structurally ASM (a sellar lesion with any of the following characteristics: diameter of >or=1 cm on magnetic resonance imaging [MRI], optic chiasmal compression on MRI, or clinical or biochemical evidence of hypopituitarism). Data from 152 patients were analyzed. Of the 152 sellar masses, 142 (93%) were pituitary adenomas. An ASM was noted in 85 of the 152 patients (56%). In the final multivariate model, male sex (odds ratio [OR], 6.23; 95% confidence interval [CI], 2.84 to 13.56; P<0.001) and self-reported visual field defect (OR, 3.62; 95% CI, 1.07 to 12.25; P = 0.039) were significantly independently associated with the presence of an ASM. The presence of new or changed headaches also tended to be associated with an ASM (OR, 2.11; 95% CI, 0.96 to 4.64; P = 0.063). Age and self-reported galactorrhea were not independently associated with the presence of an ASM and were conditionally removed from the final model. In patients with suspected sellar or pituitary disease, male sex and self-reported visual field defects independently predict the presence of an ASM. New or changed headaches also tend to be related to the presence of an ASM. The presence of predictors of an ASM should prompt expedited sellar MRI and biochemical evaluation.

Recent antiseizure medications in the Intensive Care Unit.

PubMed

Orinx, Cindy; Legros, Benjamin; Gaspard, Nicolas

2017-08-01

Seizures and status epilepticus (SE), both clinical and subclinical, are frequent in critically ill patients. The list of available antiseizure medications (ASMs) is expanding and now includes older and widely used drugs as well as more recent medications with a better safety and pharmacokinetics profile. We review a selection of recent publications about the indications and administration of ASMs in critical care for the prophylaxis and treatment of seizures and SE, focusing on recent ASMs available as intravenous formulation and emphasizing pharmacokinetics and safety issues in relation to several aspects of critical illness. Levetiracetam, lacosamide and more recently brivaracetam, represent interesting alternatives to older ASMs, mostly due to a more favorable safety and pharmacokinetic profile. Low-quality studies suggest that this profile results in better tolerability in treated patients. Ketamine might represent a useful addition in our anesthetic armamentarium for refractory SE, due to its different mechanism of action and cardiovascular properties. Little evidence is available however to support the prophylactic use of ASMs in critically ill patients, except in specific settings (traumatic brain injury and subarachnoid hemorrhage). Head-to-head studies comparing recent and older ASMs in the treatment of acute seizures and SE are ongoing or awaiting publication. Administration of ASMs to critically ill patients needs to be adapted to organ dysfunction, and especially to renal dysfunction for recent drugs. Recent ASMs and could represent better treatment choices in critically ill patients than older ones but this needs to be confirmed in randomized controlled studies. In general, further studies are required to clarify the indications and optimal use of ASMs in the critical care setting.

SDZ ASM 981: an emerging safe and effective treatment for atopic dermatitis.

PubMed

Luger, T; Van Leent, E J; Graeber, M; Hedgecock, S; Thurston, M; Kandra, A; Berth-Jones, J; Bjerke, J; Christophers, E; Knop, J; Knulst, A C; Morren, M; Morris, A; Reitamo, S; Roed-Petersen, J; Schoepf, E; Thestrup-Pedersen, K; Van Der Valk, P G; Bos, J D

2001-04-01

SDZ ASM 981 is a selective inhibitor of the production of pro-inflammatory cytokines from T cells and mast cells in vitro. It is the first ascomycin macrolactam derivative under development for the treatment of inflammatory skin diseases. This study was designed to determine the safety and efficacy of SDZ ASM 981 cream at concentrations of 0.05%, 0.2%, 0.6% and 1.0% in the treatment of patients with atopic dermatitis and to select the concentration to be used in phase III studies. This was a double-blind, randomized, parallel-group, multicentre dose-finding study. A total of 260 patients were randomly assigned to treatment with SDZ ASM 981 cream at concentrations of 0.05%, 0.2%, 0.6%, or 1.0%, matching vehicle cream, or the internal control 0.1% betamethasone-17-valerate cream (BMV). Treatment was given twice daily for up to 3 weeks. A clear dose-response relationship for SDZ ASM 981 was evident, with 0.2%, 0.6% and 1.0% SDZ ASM 981 creams all being significantly more effective than vehicle (P = 0.041, 0.001 and 0.008, respectively) in terms of baseline to end-point changes in the Eczema Area Severity Index (EASI) and pruritus score. The 1.0% cream was the most effective SDZ ASM 981 concentration. BMV was more effective than the SDZ ASM 981 creams tested in this study. It appears that the efficacy plateau was not reached with the SDZ ASM 981 creams within 3 weeks treatment. SDZ ASM 981 was well tolerated. Burning or a feeling of warmth were the only adverse events reported more frequently in the 0.6% and 1.0% SDZ ASM 981 treatment groups than in the vehicle treatment group (42.9%, 48.9% and 34.9%, respectively). Few systemic adverse events were reported during the study (headache was the most frequent systemic event reported by 15 of 252 patients) and none was considered to be related to treatment. The local tolerability profile of the 1.0% cream was similar to that of the lower concentrations. 1.0% SDZ ASM 981 cream, which was shown to be safe, well tolerated and the most effective concentration in this study, was selected as the concentration to be further developed in phase III studies.

Fetal human airway smooth muscle cell production of leukocyte chemoattractants is differentially regulated by fluticasone.

PubMed

Pearson, Helen; Britt, Rodney D; Pabelick, Christine M; Prakash, Y S; Amrani, Yassine; Pandya, Hitesh C

2015-12-01

Adult human airway smooth muscle (ASM) produce cytokines involved in recruitment and survival of leukocytes within airway walls. Cytokine generation by adult ASM is glucocorticoid-sensitive. Whether developing lung ASM produces cytokines in a glucocorticoid-sensitive fashion is unknown. Cultured fetal human ASM cells stimulated with TNF-α (0-20 ng/ml) were incubated with TNF-α receptor-blocking antibodies, fluticasone (1 and 100 nm), or vehicle. Supernatants and cells were assayed for the production of CCL5, CXCL10, and CXCL8 mRNA and protein and glucocorticoid receptor phosphorylation. CCL5, CXCL10, and CXCL8 mRNA and protein production by fetal ASM cell was significantly and dose-dependently following TNF-α treatment. Cytokine mRNA and protein production were effectively blocked by TNF-α R1 and R2 receptor neutralizing antibodies but variably inhibited by fluticasone. TNF-α-induced TNF-R1 and R2 receptor mRNA expression was only partially attenuated by fluticasone. Glucocorticoid receptor phosphorylation at serine (Ser) 211 but not at Ser 226 was enhanced by fluticasone. Production of CCL5, CXCL10, and CXCL8 by fetal ASM appears to involve pathways that are both qualitatively and mechanistically distinct to those described for adult ASM. The findings imply developing ASM has potential to recruit leukocyte into airways and, therefore, of relevance to childhood airway diseases.

Endocrine regulation of airway contractility is overlooked.

PubMed

Bossé, Ynuk

2014-08-01

Asthma is a prevalent respiratory disorder triggered by a variety of inhaled environmental factors, such as allergens, viruses, and pollutants. Asthma is characterized by an elevated activation of the smooth muscle surrounding the airways, as well as a propensity of the airways to narrow excessively in response to a spasmogen (i.e. contractile agonist), a feature called airway hyperresponsiveness. The level of airway smooth muscle (ASM) activation is putatively controlled by mediators released in its vicinity. In asthma, many mediators that affect ASM contractility originate from inflammatory cells that are mobilized into the airways, such as eosinophils. However, mounting evidence indicates that mediators released by remote organs can also influence the level of activation of ASM, as well as its level of responsiveness to spasmogens and relaxant agonists. These remote mediators are transported through circulating blood to act either directly on ASM or indirectly via the nervous system by tuning the level of cholinergic activation of ASM. Indeed, mediators generated from diverse organs, including the adrenals, pancreas, adipose tissue, gonads, heart, intestines, and stomach, affect the contractility of ASM. Together, these results suggest that, apart from a paracrine mode of regulation, ASM is subjected to an endocrine mode of regulation. The results also imply that defects in organs other than the lungs can contribute to asthma symptoms and severity. In this review, I suggest that the endocrine mode of regulation of ASM contractility is overlooked. © 2014 Society for Endocrinology.

Fetal human airway smooth muscle cell production of leukocyte chemoattractants is differentially regulated by fluticasone

PubMed Central

Pearson, Helen; Britt, Rodney D.; Pabelick, Christine M.; Prakash, Y.S.; Amrani, Yassine; Pandya, Hitesh C.

2016-01-01

Background Adult human airway smooth muscle (ASM) produce cytokines involved in recruitment and survival of leukocytes within airway walls. Cytokine generation by adult ASM is glucocorticoid-sensitive. Whether developing lung ASM produces cytokines in a glucocorticoid-sensitive fashion is unknown. Methods Cultured fetal human ASM cells stimulated with TNF-α (0–20 ng/ml) were incubated with TNF-α receptor-blocking antibodies, fluticasone (1 and 100 nm), or vehicle. Supernatants and cells were assayed for the production of CCL5, CXCL10, and CXCL8 mRNA and protein and glucocorticoid receptor phosphorylation. Results CCL5, CXCL10, and CXCL8 mRNA and protein production by fetal ASM cell was significantly and dose-dependently following TNF-α treatment. Cytokine mRNA and protein production were effectively blocked by TNF-α R1 and R2 receptor neutralizing antibodies but variably inhibited by fluticasone. TNF-α-induced TNF-R1 and R2 receptor mRNA expression was only partially attenuated by fluticasone. Glucocorticoid receptor phosphorylation at serine (Ser) 211 but not at Ser 226 was enhanced by fluticasone. Conclusion Production of CCL5, CXCL10, and CXCL8 by fetal ASM appears to involve pathways that are both qualitatively and mechanistically distinct to those described for adult ASM. The findings imply developing ASM has potential to recruit leukocyte into airways and, therefore, of relevance to childhood airway diseases. PMID:26331770

V27 Test Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stofleth, Jerome H.; Tribble, Megan Kimberly; Crocker, Robert W.

2017-05-01

The V27 containment vessel was procured by the US Army Recovered Chemical Material Directorate ( RCMD ) as a replacement vessel for use on the P2 Explosive Destruction Systems. It is the third EDS vessel to be fabricated under Code Case 2564 of the ASME Boiler and Pressure Vessel Code, which provides rules for the design of impulsively loaded vessels. The explosive rating for the vessel, based on the Code Case, is nine (9) pounds TNT - equivalent for up to 637 detonations . This report documents the results of explosive tests that were done on the vessel at Sandiamore » National Laboratories in Albuquerque New Mexico to qualify the vessel for explosive use . The primary qualification test consisted of si x 1.5 pound charges of Composition C - 4 (equivalent to 11.25 pounds TNT) distributed around the vessel in accordance with the User Design Specification. Four subsequent tests using less explosive evaluated the effects of slight variations in orientation of the charges . All vessel acceptance criteria were met.« less

A Crack Growth Evaluation Method for Interacting Multiple Cracks

NASA Astrophysics Data System (ADS)

Kamaya, Masayuki

When stress corrosion cracking or corrosion fatigue occurs, multiple cracks are frequently initiated in the same area. According to section XI of the ASME Boiler and Pressure Vessel Code, multiple cracks are considered as a single combined crack in crack growth analysis, if the specified conditions are satisfied. In crack growth processes, however, no prescription for the interference between multiple cracks is given in this code. The JSME Post-Construction Code, issued in May 2000, prescribes the conditions of crack coalescence in the crack growth process. This study aimed to extend this prescription to more general cases. A simulation model was applied, to simulate the crack growth process, taking into account the interference between two cracks. This model made it possible to analyze multiple crack growth behaviors for many cases (e. g. different relative position and length) that could not be studied by experiment only. Based on these analyses, a new crack growth analysis method was suggested for taking into account the interference between multiple cracks.

MicroRNA-20b-5p inhibits platelet-derived growth factor-induced proliferation of human fetal airway smooth muscle cells by targeting signal transducer and activator of transcription 3.

PubMed

Tang, Jin; Luo, Lingying

2018-06-01

Pediatric asthma is still a health threat to the pediatric population in recent years. The airway remodeling induced by abnormal airway smooth muscle (ASM) cell proliferation is an important cause of asthma. MicroRNAs (miRNAs) are important regulators of ASM cell proliferation. Numerous studies have reported that miR-20b-5p is a critical regulator for cell proliferation. However, whether miR-20b-5p is involved in regulating ASM cell proliferation remains unknown. In this study, we aimed to investigate the potential role of miR-20b-5p in regulating the proliferation of fetal ASM cell induced by platelet-derived growth factor (PDGF). Here, we showed that miR-20b-5p was significantly decreased in fetal ASM cells treated with PDGF. Biological experiments showed that the overexpression of miR-20b-5p inhibited the proliferation while miR-20b-5p inhibition markedly promoted the proliferation of fetal ASM cells. Bioinformatics analysis and luciferase reporter assay showed that miR-20b-5p directly targeted the 3'-UTR of signal transducer and activator of transcription 3 (STAT3). Further data showed that miR-20b-5p negatively regulated the expression of STAT3 in fetal ASM cells. Moreover, miR-20b-5p regulates the transcriptional activity of STAT3 in fetal ASM cells. Overexpression of STAT3 reversed the inhibitory effect of miR-20b-5p overexpression on fetal ASM cell proliferation while the knockdown of STAT3 abrogated the promoted effect of miR-20b-5p inhibition on fetal ASM cell proliferation. Overall, our results show that miR-20b-5p impedes PDGF-induced proliferation of fetal ASM cells through targeting STAT3. Our study suggests that miR-20b-5p may play an important role in airway remodeling during asthma and suggests that miR-20b-5p may serve as a potential therapeutic target for pediatric asthma. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

14 CFR 330.31 - What data must air carriers submit concerning ASMs or RTMs?

Code of Federal Regulations, 2010 CFR

2010-01-01

... combination passenger/cargo carrier, you must have submitted your August 2001 total completed ASM report to... correct an error that you document to the Department, you must not alter the ASM or RTM reports you...

Processing and Dynamic Failure Characterization of Novel Impact Absorbing Transparent Interpenetrating Polymer Networks (t-IPN)

DTIC Science & Technology

2014-02-01

samples were placed into the oven for the same curing treatment as before. The scanning electron microscope (SEM) photo in Figure 19 shows a typical...Interpenetrating Polymer Networks with Polyurethane and Methacrylate-based Polymers,’ S. A . Bird , PhD Dissertation, Department of Polymer and Fiber Engineering...Jajam, H. V. Tippur, S. A . Bird , and M. L. Auad, Proceedings of the 50th SES Annual Technical Meeting and ASME-AMD Summer Meeting, Providence, RI

Modeling and Characterization of Phonon Transmission and Generation across Engineered and Strained Interfaces for Developing Structure-Property Relations of Functional Nanostructures

DTIC Science & Technology

2015-05-21

translation of; report supersedes; old edition number, etc. 14. ABSTRACT. A brief (approximately 200 words) factual summary of the most significant... Chemistry C, Vol. 117, pp. 24716-24725, 2013. 21. Jose Ordonez-Miranda, Ronggui Yang, and J.J. Alvarado-Gil, A New Constitutive Equation for Nano-to...for attending ASME/IMECE 2012 in Houston, TX. Jun Liu, March 2012, “Most Excited Molecules” for his presentation at 12th GEAR2S Conference

A non-isotropic multiple-scale turbulence model

NASA Technical Reports Server (NTRS)

Chen, C. P.

1990-01-01

A newly developed non-isotropic multiple scale turbulence model (MS/ASM) is described for complex flow calculations. This model focuses on the direct modeling of Reynolds stresses and utilizes split-spectrum concepts for modeling multiple scale effects in turbulence. Validation studies on free shear flows, rotating flows and recirculating flows show that the current model perform significantly better than the single scale k-epsilon model. The present model is relatively inexpensive in terms of CPU time which makes it suitable for broad engineering flow applications.